LOCUS       DAOQIE010000001        35293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_489_length_35293_cov_7.261820, whole genome shotgun sequence.
ACCESSION   DAOQIE010000001 DAOQIE010000000
VERSION     DAOQIE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 35293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35293
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_35293_cov_7.261820"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(579..1475)
                     /locus_tag="PK144_00005"
     CDS             complement(579..1475)
                     /locus_tag="PK144_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF64134.1"
                     /translation="MNALEDMQLFATTVEAGSFTAAAERLGLSKQFVSRRLAALEARL
                     GVRLLNRTTRRLSVTELGREYHARACAILDAVAETEDMIGSGSRQPRGTLRLAAPMSF
                     GTLHLSPLLPRFLARYPDLRLEIDLNDRTVDLIGEGYDMAIRIGALPDSALVARRLAG
                     MRLVTCCSPGYLHGRGAPPEPEALSAHDCLPYGHTRATEWLYRRAGERIAVPVNGRLR
                     VNNGELARDAALAGLGLTQLPTFIVGAALARGELVTVLDDFAPNPGGIFAVYPQHRQA
                     FLAIRALADFLKEAFGEETDWA"
     gene            1618..2103
                     /locus_tag="PK144_00010"
     CDS             1618..2103
                     /locus_tag="PK144_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF64135.1"
                     /translation="MSTNTYAAASVTTAATTVTTAATGAVNRYAPAAGRVLLGGLFLV
                     SGLGKLAAPAATIGYITAAGLPAPELAYAGALAVEIGLAGALIAGYRTQLVALLMAVF
                     TLVTAFAFHFQPADQGQVIHFLKNLAITGGLLQVAAFGAGAFSLDALRSRRLRPAIAA
                     A"
     gene            2301..3017
                     /locus_tag="PK144_00015"
     CDS             2301..3017
                     /locus_tag="PK144_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007947050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF64136.1"
                     /translation="MTAILRKAGDRGVAEHGWLSSRHTFSFGHYDDPRHRGFSDLLVI
                     NDDRVAPGMGFGQHPHRDMEIFSYVLDGALEHRDTLGNGSVIRPGDVQMISAGRGIAH
                     SEFNHSRSDEVHFLQIWIVPEVRGIAPQYQQQHFDAASKRGRLRVILSPDGREGSLKL
                     HQDAVVHAGLFADDETASLAIAGGRVAYLHVARGSVQVNGVALQAGDGLKLIDAGAVN
                     ISDGQDAEVLVFDLRPETRH"
     gene            3140..3562
                     /locus_tag="PK144_00020"
     CDS             3140..3562
                     /locus_tag="PK144_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HNF64137.1"
                     /translation="MNVLYRTEATATGGRTGTARTADGAFEVRLVTPKELGGPGGSGN
                     NPEQLFAAGYSACFLGAIKFVAGQKKVKIAEDSTVTAQVGIGPRDDGQGFGLDVALSI
                     DLPGLDRETAEAIVQQAHIVCPYSHATRGSLDVRLSLK"
     gene            3642..4772
                     /locus_tag="PK144_00025"
     CDS             3642..4772
                     /locus_tag="PK144_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNF64138.1"
                     /translation="MRRNPDAARYRANLQGEIDSAALYRAMADSAGDAAQAELYRRLA
                     AVEDAHAAFWQRRLKAVGVDTKRERPGWRTRVLIGLARRFGADFVLPAARSIEAMDQT
                     GYDHQPESEHTVLPAQERSHVRLLGQLARRPRPGWTGPTYARLEGRHGAGGGNALRAA
                     VLGANDGLVSTLSLVMGVAGAQAESHTILLTGLAGLLAGACSMAMGEWISVKSANELY
                     ERQIAAEAEELREVPNEERAELELIYRSKGFTEDEARTIAERVMADPDTALSTLAREE
                     LGIDPEERSGSPWAAATASFAIFVVGATLPVLPFVWLTGERAVQASAAAGGAGLFLIG
                     AAITLFTGRNAAWSGLRQLLIGLAAAGVTWGAGRLFGVALGG"
     gene            complement(4800..4987)
                     /gene="ssrS"
                     /locus_tag="PK144_00030"
     ncRNA           complement(4800..4987)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK144_00030"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5000..5314)
                     /locus_tag="PK144_00035"
     CDS             complement(5000..5314)
                     /locus_tag="PK144_00035"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNF64139.1"
                     /translation="MSENAQLVSVHIMDADYKVSCPPADLPELQQAAAFLNARIAEIR
                     ASGRVSGSERIIAIASLNLAYDYLQLLRETSADRDEVRQRAEKLLSQVESALGKITET
                     PL"
     gene            complement(5372..5860)
                     /locus_tag="PK144_00040"
     CDS             complement(5372..5860)
                     /locus_tag="PK144_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64140.1"
                     /translation="MSTSLSPAAAPAVAALEHRLEELLRACKRLAAENRSLHLRAGQL
                     ENELATARSLHASDTDTLRRQCETIASERDQRHMTAIAELDALHEARLDAVRESLEAE
                     LAEACAAAAHERSELEAAHAQALAAAHAAADTEIAELHEQLAAARQRIGLMVARLRAL
                     EV"
     gene            5987..6562
                     /locus_tag="PK144_00045"
     CDS             5987..6562
                     /locus_tag="PK144_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNF64141.1"
                     /translation="MTTRLPNPDHDALQSALARAGSLSLAAEAHGLLCGLLCADAGLP
                     ARSWREVLLTELGDIAAVDAEGSRLLDELYAATRAGFADEELGFEPLLPEDDAPLAAR
                     AEALGQWCQGFNYGLARAGERLATLGTEAREVLDDLREFAQADYETDDADEEGEAAWV
                     ELVEYLRIGVLLLYATLAAPAPAAESGRRLH"
     gene            6633..7952
                     /gene="pepP"
                     /locus_tag="PK144_00050"
     CDS             6633..7952
                     /gene="pepP"
                     /locus_tag="PK144_00050"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNF64142.1"
                     /translation="MASFRIPAEEYAARRARLMAAMGEGSIALVAAATERVRNHDVHY
                     PFRQDSDFRYLTGFPEPDALAVLRPGHAEPYVLFCRPRDPLMEIWHGRRAGPAGAIER
                     FGADAAYPLDELDQRLPELLEGVERVHYRYGADPALDTRVSGWISALRAKLRSGVHVP
                     RELVNLDALLHAHRRVKSPAELEVMRSAGRISAAGHRRAMQVCRPGLNERRLEAEILH
                     EFALAGAGWSYPAIVGGGDNACILHYTENDAELRDGDLVLIDAGCELDSYAGDITRTF
                     PVNGRYSGPQRALYEVVLAAQHAAMAVVRPGMAWPDYHDAAVRVLTEGLISLGLLEGG
                     VDERIADESYRRFYMHRTGHWLGMDVHDPCEYKDGADWTRLEAGMVLTVEPGLYVAPD
                     AEGVDARFRGIGIRIEDDVAVTADGCEVLSAGVPTAIDDIEALMRDA"
     gene            8071..9396
                     /gene="ubiH"
                     /locus_tag="PK144_00055"
                     /gene_synonym="visB"
     CDS             8071..9396
                     /gene="ubiH"
                     /locus_tag="PK144_00055"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNF64143.1"
                     /translation="MGSKRSEDTAVTGSGPVVREDMAPAAAGASAAGAAGDYDVIVVG
                     GGMAGAALAIALDGLGLKLALVEASPWHADAHPGYDERTLALSWGSRRIFDGLGVWTA
                     IAAQATPIRQIDISDRGHIGHARLDARDQGTPALGYVAAARTIGAALIGRLERLAHVT
                     LLCPAELEHLSPGAQHCEVTVRVGEQLHSLRARLVVAADGARSRIRTQLGVPTLEWSY
                     GQTAVTANVTPEHDHGNVAYERFTEDGPLALLPMEGGRCALVLTVPDAEVEPVLAMPD
                     AQFQYLVQRRFGLRLGRFVRAGRRSAHPLKLVKAREHRHGRVAFIGNAAHTLHPIAGQ
                     GFNLGLRDVAVLAEVIADALAADEDPGSSAVLTRYADWRRWDQRATIGFTDALVRVFA
                     NPLPPVRLARNLGLIGFDLLPPVKRLLVRQLMGVAGRQPRLGLGRALAG"
     gene            9445..10755
                     /locus_tag="PK144_00060"
     CDS             9445..10755
                     /locus_tag="PK144_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HNF64144.1"
                     /translation="MGNTAASPVSTASPASTAAAAAAPGTVYDVIVVGGGMVGSALAC
                     ALGEHGLDVALIEAEPVPPLMAGEPAAARVSAISAVSQRILARLGAWPAIEQLPGAGG
                     GAPEPRVCAYTDMEVWDASGPGLIHFDAAGIGEPALGWIIENRVIQSALLARAATIDA
                     LDVYPGVAVTGFEEGRNALGVQLADGRRLSARLLVGADGAQSPLRAWAGIDTMGWGYE
                     QQAVVCRVRCEQPHHDTCWQRFLPGGPLAFLPMYDGSCSIVWSCSRAQARQVLALDDA
                     GFAHALGEAFSHRLGPVLWSGPRAAFPLRLQHAQHYVQPRVALVGDAAHVVHPLAGQG
                     VNLGLLDAAALAELLIERHGAGQDIGAIAGLRRYERARKGDNLLMQAAMDGFKRLFGS
                     PLAPVVFARNAGLLATNALGPLKHLFARRALGFGRELPALARPG"
     gene            10897..12300
                     /locus_tag="PK144_00065"
     CDS             10897..12300
                     /locus_tag="PK144_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNF64145.1"
                     /translation="MLSSLPLLAALLTAAPAAAEVASSPATGQDVSLTIYHADLALVR
                     DVRRVTLPTGDSRLAITGVSTQIQPETALLASDRPLLVLSQHYRNDALSGDTLLERFV
                     GREIEAVRVNPASGEEQREKAVLLSVREGQAVFRIGERIESDGALSPWRLVVERGQDV
                     REEPTLLADLNSSVGGEHLIELSYLSRGFSWDADYVLRLNPGNDGMDLLAWATLHNAS
                     GTTFTNARLSLVSGEVNRIAGMRGEAAADLRSAPDLPGGMPGGAAADDRLFEYQLYTI
                     ERPVTLPARQSVHVPLFSTSRAPVRKLYRTTAPLALFGAVIGNQDLRIATALSFANVS
                     PGLGRPLPSGAVRVYQREPSGTGQFLGEARINPVAEGDDAIIEVGATLDVSARRRQID
                     FKRLPRDEQEAAWVIDFRNTKTEAVTVDVTQPFGGQWRIVEESLPHRRDAADTASWSV
                     PVPAGGKVTLSYRARSR"
     gene            12356..12715
                     /locus_tag="PK144_00070"
     CDS             12356..12715
                     /locus_tag="PK144_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNF64146.1"
                     /translation="MERELCALTDIPDGGTRGIRLQPGARYADLVLARRGAQVYAYRN
                     SCPHTGAPMERESGDDDFLDYQGLHIQCGIHAAIFRFEDGFCLWGPCARRRLEAVAVR
                     IDDGRVIALDVRPWLRD"
     gene            12767..13234
                     /locus_tag="PK144_00075"
     CDS             12767..13234
                     /locus_tag="PK144_00075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNF64147.1"
                     /translation="MSTGPEASPHCSRIKVRGYHLDLYGHVNNARYLEFLEEARWAWF
                     ESGGELDRLAQRGLGFAIVRIDISYRRPAFMGEELEIRTALARIGRASGTLRQEVVLA
                     PEPAVRIAGADVTFCITDIAGGRALAFDAGLLALLGLPPAADGRRSDADDAGA"
     gene            13221..14096
                     /locus_tag="PK144_00080"
     CDS             13221..14096
                     /locus_tag="PK144_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF64148.1"
                     /translation="MPGPEAARSLVPVLAGGGTRLPAHVGVLAALADFGISVPSYVGV
                     SGGSIVAALAAAGWPPPRLLRLALDTDFRRFRGRSLWQLARRGGLSSGEAFERWLDEL
                     LEGRRFRDLPKDLAVVATDVHTSQPVVFSRKTTPDERIARAVRCSMGIPLLFAFQPYR
                     DHLLVDGSILSEDALQRDWSGDGTPVCFFRLRGEPDTRPLSTAGLFPLPAYVSMLIRT
                     FMTTLSREFVDARHWPRTVVVEVGSYSPIDFALREADKHELYACGYETVRNILPFKFP
                     TLCPPPADAVLPDAS"
     gene            14219..14611
                     /locus_tag="PK144_00085"
     CDS             14219..14611
                     /locus_tag="PK144_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNF64149.1"
                     /translation="MNRSSVTLHHARLWQGLGWALVAIVVWLSLDPSPPAPPDFLAWD
                     KAQHALAYGTLAWWFRQSFAPHRRWPLFLVGLGIGLEFLQGLGGVRMFDVDDMLANTV
                     GVGLGTALAVWTPAGRLLGWADRRLAPA"
     gene            14649..14969
                     /gene="sugE"
                     /locus_tag="PK144_00090"
     CDS             14649..14969
                     /gene="sugE"
                     /locus_tag="PK144_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNF64150.1"
                     /translation="MNWLILLVAGLCEVGWAIGLKYTEGYTRLWPSVFTIAGMVVSVW
                     LLGIAMRTLPVGTAYAVWVGIGAVGTAILGIVLFDEPADAARLFSLTLIVAGIVGLKL
                     ASAH"
     gene            15126..19253
                     /locus_tag="PK144_00095"
     CDS             15126..19253
                     /locus_tag="PK144_00095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF64151.1"
                     /translation="MTLSTDPLAAWQALPEPARVFADALAVLYEPLAFAHWPELYAGL
                     GFAKKGAAAQKAVQSLPDTLRPLLRPKAGRDPGPPVLDAAVSERLMRRLGREPEALRR
                     FADQVLRAVPAASLGAALAGRVDPAVFQQLLATSLIVNHSRRDLRLAVHANDFDTALR
                     IAREMQNGDPDRWFMERPLTQLVRCAYDADWLFQREPRLRWMMIEDWLGMLVGDLEDP
                     REARAMLDRCLSVPQVLPADQHLAQELALWCGEPLSAPMPDVAACRSFIEAGAAAALP
                     LFESAQEAWRQATGNPQQHLYGLPAFLHVCALLAVHGIGAIRDISKHLGYCSMSAVPA
                     ADVILGTALAALADEGAYPPASLPEEPDLTCLFDLLMRRWLGQPPVPGETAMLETIIA
                     RASAAGYGWIAAEARAVLARCRGEPAQSALLDVVPPKPAWQRALNRLIALGDGPAARV
                     RLIWGIDRQGGRLRLELREQKPAARGGWTPGKQVELYRYETRSSQEALLPEDRAALHS
                     LGGIHDLWHDDHVVRLLGALTGHPRVYWIGKPGEWLELVEVPPRLSLRRDKSAEGGWL
                     LELLPAPKKPGAVWQLARVKNTSRVEYTVYTHEQQRLGAVLDNGRLHVPEAGRAELAR
                     TLQALDRVLPAGAALDAVLGDDDVQPGDPTPCLLLAPAGDGLRAQLLVEPLAGLARQR
                     CGEGATVLLGERDGRPLRVRRDPAAERAARAQLLEACPTLAGLEEDDTLVLPEPQAAL
                     GLLAELRTLVTPASTEQAAAGDALPATTPGEVPAVRVVWPHGESLRLRKVLHVRDLSL
                     SLSGEGAWLQVGGSVAVDEQRVLALSALAAAALQGRYVALGNGEYVQLDAALRRRLAD
                     LGALAESGRGAGLRLPVAGLPLVEEIAAEAGAVEAKAWWQQRAGRLAEARALQAPVPS
                     TLQAQLRDYQIEGYRWLRRLAHWGAGACLADDMGLGKTVQTLALLLARAEGGPALVVA
                     PTSVCANWIAEAAHFAPSLALHDYAGSGRAGRLDGLGAGDVLVCSWALLARDAGALAA
                     VSWHTAVLDEAQAMKNAQTQRARAACALNADFRVALSGTPVENHAGEIWSLMRFLNPG
                     LLGSRERYAERYANPIERSSDPEARARLRRLLAPFLLRRLKSQVLDELPPRIEIVRSV
                     ELSDGERAFYEALRREALARASGDGGGENERRMRLFADLMRLRRACCHPQLVVPDSDL
                     PSSKLAAFGEIVGELRENRHRALVFSQFVDHLAHVRAWLDAEGIRYCYLDGSTPAAER
                     SRQVAAFQSGHGELFLISLRAGGTGLNLTAADYVIHLDPWWNPAVEDQASDRAHRLGQ
                     TRPVTVYRLVAQDTLEERIVELHRRKRDLAESLLADAGDSRLLSAEDLLALLREP"
     gene            19531..21024
                     /locus_tag="PK144_00100"
     CDS             19531..21024
                     /locus_tag="PK144_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64152.1"
                     /translation="MTLDDEPGLGAGFDVLAGLIDAVPVGALLADVRGTIRLVNRELA
                     DTLGYDATELVGRPVEDLLPETLRAAHAGWRTGFLQQPQRRPMGQGRALHARMSDGSE
                     LPVEVGLSPLVLGSERFVLATLTDLTANRQAELLFRQVVDGAPYGVVVVDGSGCIVLA
                     NPQADALFGYATHELLGRPIESLLPERYRSAHAGLREQYGRDRQMRRMGAGRDLTGLH
                     RDGSEFPVEVGLSPITLHGETCMLAAITDITERKKMELDLRKTNANLEEFTYVASHDL
                     RSPLRGISDLLSWVREDLGEAPAPAVVKNLDRITLRVQRMEQLIDNLLSYARAGRAAT
                     DFRELELDPLLDRVCELVQLPPGFELRRELVEIRLFSPPTPLETVLRNLIANAIKHHD
                     REHGLVRIGCTAEGSYCHLTVTDDGPGIPKPAQERIFKLFQTLTAAERGGSGIGLAVA
                     RRLAETHGGRLELTSDPELRQTTFHVWWPRFQRRDLHDDKNESAARR"
     gene            20993..21415
                     /locus_tag="PK144_00105"
     CDS             20993..21415
                     /locus_tag="PK144_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64153.1"
                     /translation="MTRMNLLLVDDDDVATEAVLRSLAKCDLKPVVVTAEDGLEALHV
                     LRGTHAGKRIDRPYLVLLDLNMPRMNGFEFLENLRADERLRDTVVFVLTTSDADTDRT
                     RAYHENIAGYMVKSCIGPQFRHLVSLLDVYQQTVRLPG"
     gene            21422..23185
                     /locus_tag="PK144_00110"
     CDS             21422..23185
                     /locus_tag="PK144_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNF64154.1"
                     /translation="MPDFRIPLPWPETTDKAQLRLLVVDDDDVDRERVRRMLLRTAPD
                     ATVVEACDPEETLALLQEQAFDCMIVDYRLGRLTGLDLMDLIREVVPQRCATIMVTGL
                     GDEEIAGAAVRSGINDYLTKNQLDPVGLMRSVTGAMRRTALERRLHELAYYDALTGLA
                     SRTLLTDRLQQLIARRAREPGMLAALAYIDLDHFKPINDRHGHHAGDQVLGVIAQRLK
                     GAIRASDTAARLGGDEFALLLTEVESAEDCEAVLERISRLLREPIRLDGRSDGQTVQV
                     TASIGVVLIQDDSLEADSVLRHADHMMYEVKNAGREGVRFFDPQVERRLKAAHDELEA
                     VAAGLRNGEFELFYQPQIDLATRAPTGLEALIRWRHPQRGLLAPDAFWNALQHKKLSA
                     TLGEWVIASACRQIDAWLAAGLRLKVSVNVSVHHLLHPSFAARLGAILAATPSVPPGY
                     LELEILETVAIDDFESTVAVIEACKALGVSVALDDFGTGYSSLTHLRSLPLDALKIDR
                     SFIASMLDSPSDQAIVLGILGLCRAFNRRAIAEGVESEAHIRRLVELGCHCGQGYGIA
                     RPMPAAEVAGWIERQRACVAA"
     gene            complement(23201..23872)
                     /locus_tag="PK144_00115"
     CDS             complement(23201..23872)
                     /locus_tag="PK144_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64155.1"
                     /translation="MHRRPPVASLLAPLAATLLAALSAAPAHAVPVTDPAGDILSTYA
                     GVPAQGDVDVLFAEVIFNGSGFHLSATLADTLGQTPGALYVWGFDRGAGTERFATLPG
                     GVGVLFDSVVVIDALGTATVRTLAPGVAVTATLSASLVGDTLSVDVPLSALPSAGFAP
                     AAYTWNLWPRVGSGNNNQITDFAPDGNADPALANALVTVPEPASLALAAGGLGLIGRR
                     RLRHR"
     gene            24052..>26146
                     /locus_tag="PK144_00120"
     CDS             24052..>26146
                     /locus_tag="PK144_00120"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNF64156.1"
                     /translation="MAEAASAPSASAAGARRAGWLRQSRFWLLLAAALLLAGSTGLAL
                     HQPPHPDAWRPPPGWLDPAFWRWPFERNAFMRLPLLAAPINALHVADDGTTWLLTGAG
                     EILHMSGDGLRWQRQWPVATQAPDARAAGSAIPGIAVAHAQAMPPLDGAIGKGSEVPN
                     QAAPQSPAAPAYPKPGPTAPVQQTPMTGEAPQPRAPALNALHFLSDGRHGWAVGDAGT
                     LLVTDDGGEHWQRIELAEAGEKVLRSIWFVDAQYGWIVGDDGLRLRTIDGGRSWQPPG
                     PTRALPLPRLNAVRFIDPLHGWAAGEQGTLLASVDGGASWLTLTTGTQASLTAIDVVA
                     GGDRISVVGLEGTLLQLSLAKPFAPQPVKLGGMNALDVRFAADGRRGWIGAQDGWLLV
                     TDDGGQRWQRQRLGEDNLTALAVSADGRQQRVGALSGVLQARLDDARFVALTRGGERQ
                     ARRLAGSRDGRLFLLGPYGDVLVTDATSGNWQPLVTGSGHTLLDVCLAADGLRGWLVG
                     TSGTLLATADGGRHWQPLASGTGQNLLALDCSADGRRVVAAGQYGVLLASADGGVNWQ
                     VQAQGMSDLVAVRLTADKLQATATSSDGRTLTMTDGGADWTAGGRAHEGLMLHTVRTA
                     ADVRRRWAIDLGGRLWSTADAGADWQRVAGLPEGTPALYDVEVLPDGRRLFVVGVDGT
                     VLRSLDGGTTWQKPDV"
     assembly_gap    26147..26246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26791..28215
                     /locus_tag="PK144_00125"
     CDS             26791..28215
                     /locus_tag="PK144_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNF64157.1"
                     /translation="MRPAAFAQGLAALIAALTALTAAVRAVRAFGVRPEALLASLATK
                     TRVSDLGAQTSFRYRYRQEFAEVTQALAPRTMLILIDDLDRCQPTQVLEMLEVVNFLV
                     SAGDCFIVLGIDKEKVQGALGLAFRELAQEMVDQGSDAADTRSDEARARDKRQRYARD
                     YLEKLINLEVPVPLLDGTDPARGRGMLAGESAAREQAGLRLHRWRERLGSGLRVLAGL
                     FLFIAAAAGGLYLAGLTPALPPPATVGPATTTPAGSSGAAGGDTRPVPAGSSAPMPAA
                     QGAASVIGQQAGLPWPLLVAVPALALLLGGLVAALRRPDIVVRDSPRFGEALDIWYPY
                     LLSHHRPTPRTLKRYQNRIRYVAMRQRPAEPATDPLERLLRRWLGVPAEPDSADDALP
                     EPLLVALGALQLVDPQALVAEAGALTPADATKGAAAAGSLAQARALAEAVERHRAHFG
                     DASWPPSPAAVRRFLKLSEGVAIR"
     gene            28326..28565
                     /gene="vapB"
                     /locus_tag="PK144_00130"
     CDS             28326..28565
                     /gene="vapB"
                     /locus_tag="PK144_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNF64158.1"
                     /translation="MATTTRVFRNGNAQAIRIPAELAYERTDLELEIERIGDELRIRP
                     ARRRLDGVLARFAAFSPDFMADGRGENEEAERDGW"
     gene            28567..28974
                     /locus_tag="PK144_00135"
     CDS             28567..28974
                     /locus_tag="PK144_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF64159.1"
                     /translation="MPRFMLDTNICIYLMKHHPPEVAARFAECYVGDVVMSAITLAEL
                     QYGVVCSGECRDQNAAALAALLGDIPVAAFDAGAAEAYGAIRLATRERKRDALDKLIA
                     AHALALAVTLVTNNERDFADYPGLQIENWVGSR"
     gene            complement(28997..29284)
                     /locus_tag="PK144_00140"
     CDS             complement(28997..29284)
                     /locus_tag="PK144_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNF64160.1"
                     /translation="MDRHPLRGRQAPRPEQPHAPHDAILDEQLALLLKQMAEHRQRPV
                     PELIREALGEYAERRNPGTVRKLPGVGAYRSGRSDVSGRAEEWLGEAPERA"
     gene            complement(29297..30367)
                     /gene="rlmM"
                     /locus_tag="PK144_00145"
     CDS             complement(29297..30367)
                     /gene="rlmM"
                     /locus_tag="PK144_00145"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNF64161.1"
                     /translation="MTDTTSATTLLLCCRAGFEGECAAEIQTRAAEAGFAGFAKAKPD
                     SGYVAFQPYGTDARAFAAALDFSALIFARQWIACGELLRELPVDDRVTPLLEAARGLA
                     DGPFGALRVETPDTNEAKALSAFCRPFEKAFAAAARKAGLLVDDARAPRLHLFFLSSA
                     AGWAGVSQPGNSSDWPMGIPRLRFPSGAPSRSTLKLEEAFHVFLPPDERERRLRPGMT
                     AVDLGAAPGGWTWQLVRRGLRVAAIDNGPMDETLMASGLVEHRREDGFRFRPREPVDW
                     LVCDMVEQPARIAQLVAGWIADGAAKQAIFNLKLPMKKRWAELQRCEALIAETLAGSP
                     YRLALKQLYHDREEVTGWLRRG"
     gene            complement(30376..30945)
                     /locus_tag="PK144_00150"
     CDS             complement(30376..30945)
                     /locus_tag="PK144_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64162.1"
                     /translation="MPDRVRHLVAPELPAALGADRAAAVLARLQAGGILAGVESACLP
                     EAAAGYAPGPHCAQALAEPRDARAVERLQRLAVNGVLVRSGRMVYHGSALGSAGCPHC
                     GKPLAIAGDPRWFDAVEQWLLIGEDADEGLLLCTGCTLLAPVTSWPCEPAVGFGCLGL
                     SFWNWPPLAPEFVAGLEALIGAPLIEVGD"
     gene            complement(30959..31606)
                     /locus_tag="PK144_00155"
     CDS             complement(30959..31606)
                     /locus_tag="PK144_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNF64163.1"
                     /translation="MELLAQFLDIVLHLDKHLAILAADYGPWLYAILFAIIFCETGLV
                     VTPFLPGDSLLFATGALAAGGALDVHAVFLLLMLASFLGDNTNYWIGHFLGEKVFRPD
                     ARVLKTAYLDKTHAFYEKHGGKTVLLARFAPIVRTFAPFVAGAGSMEYRRFTGFCALG
                     ALCWVGSFVYAGYLFGNIPVVKNNFTLVILGIIVVSLLPGVIEYVKHRRATAAGT"
     gene            complement(31720..32295)
                     /locus_tag="PK144_00160"
     CDS             complement(31720..32295)
                     /locus_tag="PK144_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF64164.1"
                     /translation="MQRTDFGAMSCPIARGLERVGEWWSMLILRDAFLGLSRFDEFQK
                     SLGIAPNMLARRLAHLVEAGLLEKHRYCQRPPRHEYLLTRRGEDFRPVLLTLLDFGNR
                     HYAPDGCRVQLVDTDTGEPVELALIDRRTGAEISRDRHRASPGPVDDADIGQRLAFSE
                     ALRSDPDNRPPYPGLPEPSPAAVPGPPETAS"
     gene            32426..32510
                     /locus_tag="PK144_00165"
     tRNA            32426..32510
                     /locus_tag="PK144_00165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32460..32462,aa:Leu,seq:tag)
     gene            32597..33925
                     /gene="tig"
                     /locus_tag="PK144_00170"
     CDS             32597..33925
                     /gene="tig"
                     /locus_tag="PK144_00170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNF64165.1"
                     /translation="MQVSVEELGGLERRLTVQLPAARIEQEIEQRLQDLSRRVKLDGF
                     RPGKVPLKVVKRMYGGQVRQEVLGELMQSSFQEAVSGQNLRPAAGPQISPSAAAEGDF
                     AYAATFEVLPEFELQGFEGIAVERPVAEVSDADIDSMLETLRKQRTVWTPVERPAQTG
                     DRVTVSFEGTIDGVAFPGGKGDDVPVVLGAGGMLPDFENGLIGIAAGEAREIAVSFPE
                     GYHAQELAGRTARFALNAGVVAEPSLPPVDEEFAKAFGVEDGSVESLRAALRDNMTRE
                     LGQGIKTRVKKQVMDALLAANPIELPKVLVTEEIDRIAQQAGFPAASAGNAEADKIKE
                     GVFAEEARRRVALGLLISKLVVQEKLTADPARVVEQLTTMASTYQDPNEVMQWYLKNP
                     QAMEGLRALALEDTVVDWVLARASVTEQPSTFDAVMRPAPAPAATADNAA"
     gene            33948..34583
                     /gene="clpP"
                     /locus_tag="PK144_00175"
     CDS             33948..34583
                     /gene="clpP"
                     /locus_tag="PK144_00175"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNF64166.1"
                     /translation="MYPSKGGSDIQALNLVPMVVEQSARGERAYDIYSRLLKERVIFL
                     VGPVEDYMANLVVAQLLFLEAENPDKDIHLYINSPGGSVSAGMAIYDTMQFIRPQVST
                     MCVGQAASMGALLLAGGAKGKRYCLPHSRVMIHQPLGGFSGQASDIDIHAREILLIRD
                     RLNRVLAKHTGQPLERIAQDTDRDNFMGGEAAVEYGLIDSVLSQRSAPATA"
     gene            34814..>35293
                     /locus_tag="PK144_00180"
     CDS             34814..>35293
                     /locus_tag="PK144_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNF64167.1"
                     /translation="MSKDRNDKGDDGKLLYCSFCGKSQHEVRKLIAGPNVFICDECVE
                     LCNDIIREEMEEKTPSAGSKLPKPHEIKKVLDEYVIGQDQAKKVLSVAVYNHYKRLEL
                     RRGKDEVEVSKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVE
                     "
BASE COUNT         4911 a        12514 c        12701 g         5067 t
ORIGIN      
        1 tggcaggcac gggattgcgg ttcggggcgg gcatggggac cggggcaggc acgaggcctg
       61 cccctacggg ggcatcgccg ggaaccgtcc agcctgcgaa gcatgcgcac ctggtcgtcg
      121 cagccatcga ccggccccga tcacgcatcg acctatccga aaggcttgcg gaccttgccg
      181 ccgcaggcat cgacctatcc gaaacgcttg cggaccttgc cgcgcggctt gcaggtccct
      241 gaaaaaacct tgcggatgcg tgcgcgaggc ttgcacctgc attcgcaggc aagcccggtc
      301 gatgaagttc aggcagccat cgatccggcg caggcatcga tcgctgcgcg gcaggcaggc
      361 atccctccgg cgcaggctcc gatcgctgcg cgccatcgag gcccgcatcg cccgcgcccc
      421 cgcaggccgt gcagctttgc ggcgccccgt gctgcgcatg ggtcggcggc cggcgctgac
      481 gggctggcgc ttgcgcgtgt cctgacggtc cgggcggatc gcccgcggtt ccggcgtctg
      541 ccgccgtgga ccggtgccgc gcgaggcccg cgcggcgctc aggcccagtc cgtctcctcc
      601 ccgaacgcct ccttcagaaa atccgccagc gcccggatcg ccaggaaggc ctgccggtgc
      661 tgcgggtaca ccgcgaagat gccgccgggg ttcggggcga agtcgtcgag caccgtcacc
      721 agctcgccgc gggcgagggc tgcgccgacg atgaaggtcg gcagctgcgt caggccgagg
      781 ccggcgaggg cggcgtcgcg ggccagttcg ccgttgttga cgcgcaggcg cccgttcacc
      841 ggcaccgcga tgcgctcgcc ggcgcgccgg tacagccatt cggtcgcgcg cgtgtggccg
      901 tagggcaggc agtcgtgcgc ggacagggct tcgggttcgg gcggggcgcc gcggccgtgc
      961 aggtaaccgg ggctgcagca ggtgacgagg cgcatgccgg cgaggcggcg tgcgaccagc
     1021 gccgagtcgg gcagggcgcc gatgcggatg gccatgtcat agccctcgcc gatcaggtcg
     1081 accgtgcggt cgttgaggtc gatctccagc cgcaggtccg ggtagcgggc gaggaagcgc
     1141 ggcagcagcg gcgacaggtg cagcgtgccg aagctcatcg gcgcggccag cctgagcgtg
     1201 ccgcgcggct ggcggctgcc gctgccgatc atgtcctcgg tctcggcgac cgcatcgagg
     1261 atggcgcagg cgcgggcgtg gtactcgcgg cccagctcgg tgacgctcag gcgccgggtc
     1321 gtgcggttca aaagccgcac gccgaggcgc gcctccagcg ccgcgagcct gcggctgacg
     1381 aactgcttcg acagcccgag gcgttcggcc gcggccgtga agctgccggc ctcgaccgtg
     1441 gtggcgaaca actgcatgtc ttcgagcgcg ttcattgtcc ttctccagtg gacgatgtga
     1501 cgcattctgg ctgatttatg gcgtgatcgt ggctgcgtag agtgcgctcc atcggcaagg
     1561 caccggccag accggcagcc accgaaaccg gaaacccgca ctcaagaggt catcgccatg
     1621 agcaccaata cctacgccgc cgcttccgtc accactgctg ccaccacggt caccaccgct
     1681 gcgaccggcg ccgtcaaccg ctacgccccg gccgccggcc gcgtgctgct cggcggactg
     1741 ttcctggtca gcggcctcgg caagctcgcc gcgccggcgg ccacgatcgg ctacatcacc
     1801 gccgccggcc tgccggcgcc ggaactggcc tacgccggtg cgctggcggt cgagatcggg
     1861 ctggcgggtg cgctgatcgc cggttaccgc acgcaactgg tcgcgctgct gatggcggtg
     1921 ttcacgctgg tcacggcctt cgccttccac ttccagcccg ccgaccaggg ccaggtgatc
     1981 cacttcctga agaatctggc gatcaccggc ggcctgctgc aggtcgcggc cttcggcgcc
     2041 ggcgccttca gcctcgatgc actgcgcagc cgtcgcctgc gcccggccat cgccgcggcc
     2101 tgaaacacct gatccggctg agcggatcgc aggtgcctgc cttcaagccc ctccccctcg
     2161 cgggggaggg gttgggggag agggggaaac ccccggcacc ccggccgcat ccgcccgcca
     2221 tggacgacgc ctgaatcctg aacccccggc cccggcgccc ccccgcgccg gtccgcctcc
     2281 gaagcaacgg gagcaagacc atgactgcaa tcctgcgcaa ggccggtgac cgcggcgtcg
     2341 ccgagcacgg ctggctcagc tcgcgccaca ccttctcgtt cggccactac gacgacccgc
     2401 gccatcgcgg cttctcggac ctgctggtca tcaacgacga ccgcgttgcg ccgggcatgg
     2461 gcttcggcca gcacccgcac cgcgacatgg agatcttttc ctacgtgctc gacggcgcgc
     2521 tcgaacaccg cgacacgctc ggcaacggct cggtgatccg ccccggcgac gtgcagatga
     2581 tcagcgccgg gcgcggcatc gcgcacagcg agttcaacca ctcgcgcagc gacgaggtgc
     2641 acttcctgca gatctggatc gtgcccgagg tgcgcggcat cgcgccgcag taccagcagc
     2701 agcacttcga tgcggcgagc aagcgcggaa ggctgcgggt gatcctgtcg ccggacgggc
     2761 gcgagggttc gctgaagctg catcaggacg cggtcgtcca cgccgggctg ttcgcggatg
     2821 acgaaacggc aagcctggcg attgccggcg gtcgcgtcgc ctaccttcac gtcgcccgcg
     2881 gcagcgtgca ggtcaacggc gtcgcgctgc aggccggcga cggcctgaag ctcatcgatg
     2941 ccggggccgt gaacatcagc gacgggcagg acgccgaggt gctggtcttc gacctgcgcc
     3001 ccgaaacccg gcactgaagg ccgtgaccgg tgcgccgtcc gcgagcggcg caccggccga
     3061 ttccctccac ccccgacccc cggatgcccg cctgcgtggc gggcatcgcc cacccgcgaa
     3121 gtctgcgagg agcaccgcca tgaacgtcct gtaccgcacc gaagccaccg ccaccggcgg
     3181 tcgtaccggc accgcccgca ccgctgacgg cgccttcgaa gtccggctgg tcacgccgaa
     3241 ggaactcggc ggccccggcg gcagcggcaa caaccccgag cagctgttcg ctgccggcta
     3301 ctcggcctgc ttcctcggcg cgatcaagtt cgtcgccggc cagaagaagg tgaagatcgc
     3361 cgaggacagc accgtcaccg cccaggtcgg catcggcccg cgcgacgacg gccagggctt
     3421 cggcctcgac gtggcgctga gcatcgacct gcccggcctc gatcgcgaga ccgccgaggc
     3481 catcgtgcag caggcgcaca tcgtctgccc gtactcgcac gccacccgcg gcagccttga
     3541 cgtgcgcctg agcctcaagt gagtgatccg gccgcccgca ccttgcccgc gtgcgggcgg
     3601 cctctgtcat ccgtgatccg cccgctgcga ggacgcccgc catgcgtcgc aaccccgatg
     3661 ccgcccgcta ccgcgccaac ctgcagggcg agatcgacag cgccgcgctg taccgggcga
     3721 tggccgacag cgccggcgat gccgcgcagg ccgagctgta ccggcgcctc gcggcggtcg
     3781 aggacgcgca cgcggcgttc tggcagcggc ggctgaaggc ggtcggcgtc gatacgaagc
     3841 gcgagcgccc gggctggcgc acgcgcgtgc tgatcgggct cgcgcgccgc ttcggggcgg
     3901 atttcgtgct gccggcggcg cgctcgatcg aggcgatgga ccagaccggc tacgaccacc
     3961 agcccgagag cgagcacacc gtgctgccgg cgcaggagcg atcccatgtg cgcctgctcg
     4021 gccagctcgc gcgccgcccg cgtccgggct ggaccggtcc gacctacgcc cgcctcgaag
     4081 gccgccacgg tgccggcggc ggcaacgcgc tgcgcgccgc ggtgctcggc gccaacgacg
     4141 ggctggtgtc gacgctgagc ctggtcatgg gcgtggccgg cgcgcaggcc gaatcgcaca
     4201 ccatcctgct gaccggcctc gccggcctgc tggccggcgc ctgctcgatg gcgatgggcg
     4261 agtggatctc ggtgaagagc gccaacgaac tctacgaacg ccagatcgcc gccgaggccg
     4321 aggagctgcg cgaggtgccg aacgaggagc gcgccgagct ggagctgatc taccgcagca
     4381 agggcttcac cgaggacgag gcgcgcacga ttgccgagcg cgtgatggcc gacccggata
     4441 cggcgctgtc gacgctggcg cgcgaggaac tcggcatcga ccccgaggag cgcagcggct
     4501 cgccgtgggc ggcggcgacg gcgtccttcg cgatcttcgt cgtcggtgcg acgctgccgg
     4561 tgctgccgtt cgtgtggctg acaggcgagc gcgcagtgca ggcaagcgcg gcggccggcg
     4621 gtgcggggct cttcctgatc ggtgccgcga tcacgctgtt caccggccgc aacgccgcgt
     4681 ggtcgggcct gcggcagctg ctgatcgggc tggccgcggc cggcgtgacc tggggcgcgg
     4741 ggcggctgtt cggcgtggcg ctcgggggct gagcgcggcc ggcgcaaaaa aagaaagagg
     4801 catcccccgc ctgtgccgtc ggagccttgg cccttgaacc agatggttca agtggggatc
     4861 ggcgcggtgc ctcaggcttc ccgctgctag gcggacttgc acaccggctc cggccaaccg
     4921 accccctggc agatcaaact atggatcaag gggatgcggc tctgtgacac gaacaccgca
     4981 ggggatgccc gtggccggac tataacgggg tttcggtgat tttcccaagc gccgattcga
     5041 cctgtgacag cagcttctcg gcacgctggc gcacctcgtc gcggtcggcc gaagtttcgc
     5101 gcagcagctg caggtagtcg taggcgaggt tcaggctggc gatggcgatg atgcgctcgg
     5161 agccggacac gcggccgctg gcgcggatct cggcgatgcg cgcgttcagg aaggcggcgg
     5221 cctgctgcag ttcgggcagg tcggcgggcg ggcaggacac cttgtagtcg gcgtccatga
     5281 tgtgcaccga caccagctgg gcgttctcgg acacggtgcg ggcctcggtt tcaggcggcc
     5341 ggcgggtttc cggcgggttg gacgggctgg atcagacttc gagcgcgcgc aggcgcgcga
     5401 ccatcagccc gatgcgctgg cgggcggcgg cgagctgctc gtgcagttcg gcgatctcgg
     5461 tgtcggccgc agcgtgggcg gcggcgaggg cctgcgcatg cgcggcttcg agctcgctgc
     5521 gttcgtgcgc ggccgcggcg caggcttcgg cgagttcggc ttcgagcgac tcgcgcaccg
     5581 cgtcgagccg ggcctcgtgg agagcgtcga gttcggcgat cgcggtcata tggcgctggt
     5641 cgcgctcgct ggcgatggtc tcgcactggc ggcgcagcgt gtcggtgtcg ctggcgtgca
     5701 ggctgcgcgc cgtggcgagt tcgttttcga gctggccggc gcgcaggtgc aggctgcggt
     5761 tctcggcggc gagccgcttg caggcgcgca gcagctcttc gagccggtgt tcgagcgccg
     5821 cgacggccgg ggcggcggcg ggggacaggg acgtggacat ggcgccggac tataggggcc
     5881 gctctgtgac agggtcaatc gcgggccgcc gtaccgcgtc tttacgggcc ggcgccggcc
     5941 ggggttacga taaccgtcct ttccggatgc cgtgtgcccc ccaccgatga ctacgcgcct
     6001 gccgaatccg gaccacgatg cgctgcagtc ggcgctcgcg cgtgccggca gcctgagcct
     6061 cgccgccgag gcgcacggcc tgctgtgcgg actgctctgt gccgacgccg gcctgccggc
     6121 ccgcagctgg cgcgaggtgc tgctgaccga actcggcgac atcgccgccg tcgatgccga
     6181 aggcagccgg ctgctcgatg agctctacgc ggcgacgcgc gccggctttg ccgatgagga
     6241 actcggcttc gaaccgctgc tgcccgagga cgacgcgccg ctcgccgcgc gcgccgaagc
     6301 cctcgggcag tggtgccagg gcttcaacta cggtctggcg cgtgccggcg agcggctggc
     6361 gacgctcggg accgaggcgc gcgaggtgct cgatgacctg cgcgagttcg cgcaggccga
     6421 ctacgaaacc gacgacgccg acgaggaagg cgaggcggcg tgggtcgagc tggtcgagta
     6481 cctgcgcatc ggcgtgctgc tgctgtacgc gacgctcgcc gcgccggccc cggcggccga
     6541 atccggcagg cgtctgcact gattcgccgg caccgacgct gccggtgtcc ggcattccgt
     6601 ttcgtttccc acctgcactc cgaggtcagc cgatggccag cttccgcatt ccggccgagg
     6661 aatatgccgc ccgtcgcgcc cggctgatgg cggcgatggg cgagggttcg atcgcgctgg
     6721 tcgccgcggc gaccgagcgc gtgcgcaacc acgacgtgca ctatccgttt cgccaggaca
     6781 gcgacttccg ctacctgacc ggcttcccgg agcccgacgc gctggcggtg ctgcgccccg
     6841 gccacgccga gccctacgtg ctgttctgcc ggccgcgcga tccgctgatg gagatctggc
     6901 acggccgccg cgccgggccg gccggcgcga tcgagcgctt cggggccgat gccgcgtatc
     6961 cgctcgacga gctcgaccag cgcctgccgg agctgctcga aggtgtcgag cgcgtgcatt
     7021 accgctacgg cgccgatccg gcgctcgaca cccgcgtcag cggctggatc agcgcgctgc
     7081 gcgcgaagct caggagcggc gtgcacgtgc cgcgcgaact cgtgaacctc gatgcgctgc
     7141 tgcacgcgca ccggcgcgtg aagtcgccgg ccgagctgga ggtcatgcgc tcggccgggc
     7201 gcatctcggc ggccgggcac cggcgcgcga tgcaggtctg ccggccgggc ctgaacgagc
     7261 ggcggctgga ggccgagatc ctgcacgagt tcgcgctcgc cggcgccggc tggtcgtatc
     7321 cggcgatcgt cggcggcggc gacaacgcct gcatcctgca ttacaccgag aacgacgccg
     7381 agctgcgcga cggcgatctg gtgctgatcg acgccggctg cgagctggac tcctacgccg
     7441 gcgacatcac ccgcaccttc ccggtcaacg gccgctacag cgggccgcag cgcgcgctgt
     7501 acgaggtcgt gctggccgcg cagcatgcgg cgatggccgt cgtgcgcccc ggcatggcct
     7561 ggcccgacta ccatgacgcc gccgtgcgcg tgctgaccga gggcctgatc agcctcggcc
     7621 tgctcgaagg cggggtcgac gagcgcatcg ccgatgagtc ctaccggcgc ttctacatgc
     7681 accgcaccgg ccactggctc ggcatggacg tgcacgatcc ctgcgaatac aaggacggcg
     7741 ccgactggac gcggctcgaa gccggcatgg tgctgaccgt cgagccgggc ctgtacgtcg
     7801 cccccgatgc cgaaggcgtc gatgcgcgct ttcgcggcat cggcatccgc atcgaggatg
     7861 acgtggcggt cacggccgac ggctgcgagg tgctgagcgc cggcgtgccg accgcgatcg
     7921 acgacatcga ggcgctgatg cgcgacgcct gagcacggcg tgcgcagcgc agcgcccgga
     7981 cctgacaacg acaagcgccg ccggccggtt tttccggtgc gagcggaccc atcaccggcg
     8041 ccggacggcg gcggacccga ggagcgaggc gtgggcagca agcgcagcga agacacggcg
     8101 gtgaccggca gcgggccggt cgtccgcgag gacatggcgc cggcagcggc cggcgcgtct
     8161 gccgccggag ccgccgggga ttacgacgtc atcgtcgtcg gtggcggcat ggccggtgcg
     8221 gcgctggcga tcgcgctcga cgggctgggc ctgaagctcg cactggtcga ggccagtccc
     8281 tggcacgccg atgctcaccc cggctacgac gagcgcacgc tggcgctgtc ctggggctcg
     8341 cggcgcatct tcgacgggct cggcgtctgg acggcaatcg cggcgcaggc gacgccgatc
     8401 cggcagatcg acatctccga ccgcggccac atcggccacg cccggctcga tgcccgcgac
     8461 cagggcacgc cggcgctcgg ctacgtggcc gcggcgcgca cgatcggggc ggcgctgatc
     8521 gggcggctgg agcggcttgc acacgtgacg ctgctctgcc cggccgagct cgaacatctg
     8581 agccccggcg cgcagcactg cgaggtgacg gtgcgcgtcg gcgagcagtt gcactcgctg
     8641 cgcgcgcggc tggtggtcgc ggccgacggg gcgcgctcgc gcatccgcac gcagctcggc
     8701 gtgccgacgc tggagtggag ctacggccag acggcggtga ccgccaacgt cacgcccgag
     8761 cacgaccacg gcaacgtcgc ctacgagcgc ttcaccgagg acgggccgct tgcgctgctg
     8821 ccgatggaag gcgggcgctg cgcgctggtg ctgaccgtgc ccgatgccga ggtcgagccg
     8881 gtgctggcga tgccggatgc gcagttccag tacctggtgc agcggcgctt cggcctccgg
     8941 ctcgggcgct tcgtgcgcgc cggccggcgc agcgcccatc cgctgaagct cgtcaaggcg
     9001 cgcgagcacc ggcacgggcg cgtcgccttc atcggcaatg ccgcacacac gctgcacccg
     9061 atcgccgggc agggcttcaa cctcggcctg cgcgatgtcg cggtgctggc cgaggtcatc
     9121 gccgatgcgc tggccgccga cgaggacccc ggcagcagcg ccgtgctgac gcgctacgcc
     9181 gactggcggc gctgggacca gcgcgcgacg atcggcttca ccgatgcgct ggtgcgcgtg
     9241 ttcgcgaacc cgctgccgcc ggtgcgcctg gcgcgcaatc tcggcctgat cggcttcgac
     9301 ctgctgccgc cggtcaagcg cctgctggtg cgccagctga tgggcgtcgc cggtcgccag
     9361 ccgcggctcg gcctcggccg ggccctggcc ggctgaacgt cccgctcccc gattccgcct
     9421 gactgcacgg aacctccgca tccgatgggc aacaccgctg cttcgcctgt ttccactgct
     9481 tcccctgcat ccacggccgc tgccgcagcc gcccccggga ccgtctacga cgtcatcgtc
     9541 gtcggcggcg gcatggtcgg ctcggcgctg gcctgcgcgc tcggcgagca cgggctggac
     9601 gtggcactga tcgaggccga accggtgccg ccgctcatgg ccggcgagcc ggccgcggcg
     9661 cgcgtctcgg cgatcagtgc cgtctcgcag cgcatcctcg ccagactcgg cgcctggccg
     9721 gcgatcgagc agctgccggg ggccgggggc ggcgcacccg agccgcgggt ctgcgcctat
     9781 accgacatgg aggtctggga cgcgagcggc ccgggcctga tccacttcga tgccgccggc
     9841 atcggcgagc cggccctcgg ctggatcatc gagaaccgcg tgatccagag cgcgctgctc
     9901 gcgcgcgccg ccacgatcga cgcgctcgat gtctaccccg gcgtcgccgt caccggcttc
     9961 gaggaagggc gcaacgccct cggcgtgcaa ctggccgacg gccgccggct gtcggcgcgc
    10021 ctgctggtcg gggccgacgg cgcgcagtcg ccgctgcgcg cctgggccgg catcgacacg
    10081 atgggctggg gctacgagca gcaggccgtg gtctgccgcg tgcgctgcga gcagccgcac
    10141 catgacacct gctggcagcg cttcctgccc ggcgggccgc tggccttcct gccgatgtac
    10201 gacggcagct gctcgatcgt ctggagctgc agccgcgcgc aggcgcgtca ggtgctggcg
    10261 ctcgacgatg ccggcttcgc gcacgcgctc ggcgaggcct tcagccaccg gctcgggccg
    10321 gtgctctgga gcgggccgcg cgccgccttc ccgctgcggc tgcagcacgc gcagcactac
    10381 gtgcagccgc gcgtggcgct ggtcggcgat gccgcgcacg tcgtgcatcc gctcgccggc
    10441 cagggggtca acctcggcct gctcgatgcc gccgcgctcg ccgagctgct catcgaacgt
    10501 cacggcgccg ggcaggacat cggcgcgatc gccggcctgc gccgctacga gcgcgcgcgc
    10561 aagggcgaca acctgctgat gcaggcggcg atggacggct tcaagcgcct gttcggcagc
    10621 ccgcttgccc cggtcgtgtt tgcacgcaac gccggcctgc tcgcgaccaa cgcgctcggg
    10681 ccgctgaagc atctgttcgc gcgccgcgcg ctcggcttcg gccgcgaact gccggcactc
    10741 gcccgccccg gctgagcggc ggcgcagccc gcttgcgaga ttcgactaca actcctgcgg
    10801 tatatgcgcc acaagctgtc gtaattggga tacgcttgca gtcctgccct tgccggaacg
    10861 aacggacgcc ggccatcgct gcgagcaccc gacaccatgc tttccagcct gcccctgctg
    10921 gccgccctgc tgaccgccgc tccggctgca gccgaagtcg ccagttcccc ggcgaccggt
    10981 caggacgtga gcctgaccat ctatcacgcc gacctcgcgc tggtgcgcga cgtgcggcgc
    11041 gtgacgctgc cgaccggtga cagccggctg gcgatcaccg gcgtcagcac gcagatccag
    11101 cccgaaaccg cgctgctcgc cagcgaccgg ccgctgctgg tgctgtcgca gcactaccgc
    11161 aacgatgcgc tgtccggcga cacgctgctc gaacgcttcg tcgggcgcga gatcgaggcc
    11221 gtgcgcgtca atccggccag cggcgaggag caacgcgaga aggccgtgct gctgtcggtg
    11281 cgcgaggggc aggcggtgtt ccgcatcggc gagcgcatcg aatccgacgg cgcgctgtcg
    11341 ccgtggcggc tggtcgtcga gcgcggtcag gacgtgcgcg aggagccgac gctgctcgcc
    11401 gacctcaaca gcagcgtcgg cggcgagcac ctgatcgagc tgtcctacct gtcgcgcggc
    11461 ttctcgtggg atgccgatta cgtgctgcgc ctgaaccccg gcaacgacgg catggacctg
    11521 ctcgcctggg cgacgctgca caacgccagc ggcacgacct tcaccaatgc ccggctgtcg
    11581 ctggtctccg gcgaggtcaa ccgcatcgcc ggcatgcgcg gcgaagctgc cgccgacctg
    11641 cgctcggcgc ccgacctgcc cggcggcatg ccgggagggg cagccgccga tgaccggctg
    11701 ttcgagtacc agctctatac gatcgaacgg ccggtgacgc tgccggcgcg ccagtcggtg
    11761 cacgtgccgc tgttctcgac ctcgcgcgcc ccggtgcgca agctctaccg cacgaccgcg
    11821 ccgctcgcgc tgttcggtgc ggtgatcggc aatcaggacc tgcgcatcgc cacggcgctg
    11881 tcgttcgcga acgtgtcgcc ggggctcggc cggccgctgc cttcgggggc cgtgcgcgtc
    11941 taccagcgcg agccgagcgg caccggccag ttcctcggcg aggcgcgcat caatccggtc
    12001 gccgagggcg atgacgcgat catcgaggtc ggcgccacgc tcgatgtcag cgcccgccgc
    12061 cgccagatcg acttcaagcg cctgccgcgc gacgagcagg aagccgcgtg ggtgatcgac
    12121 ttccgcaaca ccaagaccga ggcggtcacc gtcgatgtca cgcagccctt cggcggccag
    12181 tggcgtatcg tcgaggaaag cctgccgcac cggcgcgatg ccgccgacac cgcgagctgg
    12241 agcgtgccgg ttccggccgg cggcaaggtg acgctgagtt accgcgcccg cagccgctga
    12301 gcgccgtcgc gggcggcgca cgtaccgccc gtgcagaggc gggggaggag gatcgatgga
    12361 acgcgaactc tgtgcgctga ccgacatccc cgatggcggc acccgcggca tccgcctgca
    12421 gcccggcgcg cgctacgccg atctggtgct cgcgcgccgc ggcgcgcagg tctacgccta
    12481 ccgcaactcc tgtccgcaca ccggcgcgcc gatggagcgc gagtccggcg acgacgattt
    12541 cctcgactat cagggcctgc acatccagtg cggcatccac gcggcgatct tccgcttcga
    12601 ggacggcttc tgcctgtggg gcccgtgtgc gcgccggcgg ctcgaagccg tggcggtgcg
    12661 catcgacgac gggcgggtca tcgcgctcga cgtgcggccg tggctgcgcg actgaggccg
    12721 cgcgtgccaa gctgtcggtt ccgccgccga tcaggaaggg cctgccttga gcaccggacc
    12781 cgaagccagc ccgcattgca gccgcatcaa ggtgcgcggc tatcacctcg acctgtacgg
    12841 gcacgtcaac aacgcccgct acctcgaatt cctcgaagaa gcccgctggg cctggttcga
    12901 gagcggcggt gagctcgacc ggctcgcgca gcgcggcctc ggcttcgcga tcgtgcgcat
    12961 cgacatcagc taccgccggc cggccttcat gggcgaggag ctcgaaatcc gcactgcgct
    13021 cgcgcgcatc ggccgcgcca gcggcacgct gcgtcaggag gtcgtgctcg caccggagcc
    13081 ggccgtgcgc atcgccgggg ccgacgtgac cttctgcatc accgacattg ccggcggccg
    13141 ggcgctggcc ttcgatgccg ggctgctcgc gctcctcggc ctgccgccgg cggcggacgg
    13201 ccgccgcagc gatgccgacg atgccggggc ctgaggcggc gcgcagtctg gtgccggtgc
    13261 tggccggcgg cggcacgcgg ctgccggcgc acgtcggcgt gctcgccgcg ctcgccgatt
    13321 tcggcatcag cgtgccgagc tacgtcgggg tgtccggcgg cagcatcgtc gcggcgctgg
    13381 ccgccgccgg ctggccgccg ccgcggctgc tgcgtctggc gctcgacacc gacttccggc
    13441 gcttccgcgg ccgctcgctg tggcagctcg cgcgccgcgg cgggctgtcc tcgggcgagg
    13501 ccttcgagcg ctggctcgac gaactgctcg aaggccggcg cttccgcgat ctgccgaagg
    13561 acctggccgt ggtcgccacc gacgtgcaca cctcgcagcc ggtggtgttc agccgcaaga
    13621 ccacgccgga tgagcgcatc gcgcgcgcgg tgcgctgctc gatgggcatc ccgctgctgt
    13681 tcgcgttcca gccgtatcgc gaccatctgc tggtcgacgg cagcatcctg tccgaggatg
    13741 cgctgcagcg cgactggagc ggcgacggca cgccggtgtg cttcttccgg ctgcgcggcg
    13801 agcccgacac ccggccgctg tccacggccg gactgtttcc gctgccggcc tacgtgtcga
    13861 tgctgatccg caccttcatg accacgctgt cgcgcgagtt cgtcgatgcc cgccactggc
    13921 cgcgcaccgt cgtcgtcgag gtcggcagct actcgccgat cgacttcgcg ctgcgcgagg
    13981 ccgacaagca cgagctgtat gcctgcggct acgagacggt gcgcaacatc ctgccgttca
    14041 agttcccgac gctgtgcccg ccgcccgcgg acgccgtcct gcccgatgcc tcctgaagcg
    14101 gcgggcgcga cgcgcggccg gccgcggcgg ccggcaggcc ggcctgatcc ggctcgccgg
    14161 caatgccact cacaacgcat tcacgaacgc cttcacgcat gaccggtaga gactccgcat
    14221 gaaccgaagc tccgtgacgc tgcaccacgc ccgcctgtgg cagggcctcg gctgggcgct
    14281 ggtcgcgatc gtcgtctggc tgtcgctcga tcccagtccg ccggcaccgc cggatttcct
    14341 cgcctgggac aaggcccagc acgccctcgc ctacggcacg ctggcctggt ggttccgcca
    14401 gagcttcgcg ccgcaccggc gctggccgct gttcctggtc gggctcggca tcgggctgga
    14461 gttcctgcag gggctcggcg gcgtgcgcat gttcgatgtc gacgacatgc tcgcgaacac
    14521 cgtcggcgtc ggtctcggca cggcgctcgc cgtgtggacg ccggccggcc ggctgctcgg
    14581 ctgggccgac cggcggctgg cgccggcatg aggccgggct tcggagcgcc gtctgcggag
    14641 ggctggcgat gaactggctg atcctgctgg ttgccggtct ctgcgaggtc ggctgggcga
    14701 tcgggctgaa gtacaccgag ggctacaccc ggctgtggcc gagcgtgttc acgatcgccg
    14761 gcatggtcgt cagcgtctgg ctgctcggca tcgccatgcg cacgctgccg gtcggcaccg
    14821 cctacgcggt ctgggtcggc atcggcgcgg tcggcaccgc gatcctcggc atcgtgctgt
    14881 tcgacgaacc ggccgatgcc gcgcgcctgt tcagcctgac gctgatcgtc gccggcatcg
    14941 tcgggctgaa gctcgccagc gcgcactgag cgcaccggcc cggtggaagc agcgcggctt
    15001 gcgcgtggcc ggcgcttccg gcgcccgccc tgagcggatt cggaaccttc gccgccccgg
    15061 gcccggcgat gaatctgcct gtccgggagc ctgcccggac gcctgcatga ccggatgaac
    15121 cgtcgatgac cctcagcacc gatccgctcg ccgcctggca ggcgctgccc gaacccgccc
    15181 gcgtcttcgc cgatgcgctc gccgtgctgt acgagccgct cgccttcgca cactggccgg
    15241 agctgtacgc cgggctcggc ttcgcgaaga agggagcggc cgcgcagaag gccgtgcaat
    15301 ccctgccgga caccctgcgc ccgctgctgc gcccgaaggc cggacgcgat ccgggtccgc
    15361 cggtgctcga tgcagccgtg tccgaacgcc tgatgcgccg gctcggccgg gagccggagg
    15421 cgctgcggcg ctttgccgat caggtgctgc gcgcggtgcc ggccgcaagt ctgggcgcgg
    15481 cgctggccgg ccgcgtcgat cccgccgtgt tccagcagtt acttgcgacg agcctgatcg
    15541 tcaatcacag ccgccgcgac ctgcgcctgg ccgtgcatgc caacgatttc gatacggccc
    15601 tgcggattgc gcgggagatg cagaatggcg atccggatcg ctggttcatg gagcgcccgc
    15661 tgacgcagct cgtgcgctgc gcgtacgacg ccgactggct gttccagcgc gagccgcgcc
    15721 tgcgctggat gatgatcgag gactggctcg gcatgctggt cggcgatctg gaagacccgc
    15781 gcgaagcgcg ggccatgctc gatcgctgcc tgagcgtgcc gcaggttctg cccgccgatc
    15841 agcacctcgc ccaggagctg gcgctctggt gtggcgaacc gctttccgca ccgatgcccg
    15901 acgtggcggc gtgccgctcg ttcatcgagg caggtgccgc ggcggcgctg cccttgttcg
    15961 agtcggcgca ggaggcgtgg cggcaggcca ccggcaatcc gcaacagcat ctgtacggct
    16021 tgccggcctt tctgcacgtc tgcgcgctgc tggccgtgca cgggatcggc gcgatccggg
    16081 acatcagcaa gcacctgggc tactgcagca tgagtgcggt accggcggcc gacgtgatcc
    16141 tcggcaccgc gctcgctgcc ctggccgatg agggggctta tccgccggcg tccctgcccg
    16201 aagagcccga tctcacctgc ctgttcgact tgctgatgcg ccgctggctg ggccagccgc
    16261 cggtgccggg cgagaccgcc atgctcgaaa cgatcattgc gcgcgcctcg gccgccggct
    16321 acggctggat cgctgccgag gcacgggccg tgctcgcgcg ctgtcgtggt gagccggcgc
    16381 agtccgcgct gctcgatgtc gtgccgccga agccggcctg gcagcgggcg ctgaaccggc
    16441 tgatcgcact cggggacgga ccggcggccc gcgtgcggtt gatctggggc atcgaccgtc
    16501 agggcggccg gttgcggctc gaactgcgcg agcagaagcc ggcggcgcgc ggcggctgga
    16561 cgccgggcaa gcaggtggaa ctctaccgct acgaaacccg cagcagtcag gaggccctgc
    16621 tgcccgagga tcgcgccgcg ctgcattccc tcggtggcat ccatgatctg tggcacgacg
    16681 atcacgtcgt gcgcctgctc ggggcgctga ccggacatcc gcgcgtgtac tggatcggca
    16741 agcccggcga atggctggaa ctggtcgagg tgccgccgcg cctgtcgctg cgccgggaca
    16801 agtcggccga gggcggctgg ctgctcgaac tgctgccggc gccgaagaag ccgggcgcgg
    16861 tctggcagct cgcgcgcgtg aagaacacca gccgcgtcga gtacaccgtc tacacgcatg
    16921 agcagcagcg gctcggggcc gtgctcgaca acggccggct gcacgtgccc gaggccggcc
    16981 gggccgaact cgcacgcacg ctgcaggcgc tcgatcgcgt gctgccggcc ggtgccgcgc
    17041 tcgatgccgt gctcggcgat gacgacgtgc agcccggcga tccgaccccg tgcctgctgc
    17101 tggcgccggc cggcgacggc ctgcgcgcgc agctgctggt cgagccgctc gccgggctgg
    17161 cgcgccagcg ctgcggcgag ggagcgaccg tgctgctcgg cgagcgcgat ggccggccgc
    17221 tgcgcgtccg ccgcgatccg gccgccgagc gcgcggcgcg ggcgcagctc ctcgaagcct
    17281 gcccgacgct cgccggcctt gaggaggacg acacgctggt gctgcccgag ccgcaggccg
    17341 cgctcggcct gctcgccgag ctgcgcacgc tggtgacccc cgccagcacg gagcaggcgg
    17401 ccgcgggcga tgcgctgccg gcaaccacgc ccggcgaggt gccggccgtg cgcgttgtct
    17461 ggccgcacgg cgaatcgctg cgcctgcgca aggtgctgca cgtgcgcgac ctgtcgctgt
    17521 cgctgtccgg cgagggcgcg tggctgcagg tcggcggttc ggtcgcggtc gatgagcagc
    17581 gcgtgctggc gctgtcggcg ctcgcggccg ccgcgctgca gggccgctac gtcgcgctcg
    17641 gcaacggcga atacgtgcag ctcgatgccg cgctgcgccg ccggctcgcc gatctcggcg
    17701 cgctcgccga gtccggccgc ggcgccgggc tgcgcctgcc ggtcgccggc ctgccgctgg
    17761 tcgaggaaat cgccgccgaa gccggcgcgg tcgaggcgaa ggcctggtgg cagcagcgtg
    17821 ccgggcggct tgccgaggca cgtgcgctgc aggcaccggt gccctcgacg ctgcaggcgc
    17881 agctacgcga ctaccagatc gagggctacc gctggctgag gcggctcgcg cactgggggg
    17941 ccggggcctg tctggccgac gacatggggc tcggcaagac cgtgcagacg ctggcgctgc
    18001 tgctcgcgcg cgccgagggc gggccggcgc tggtggtggc gccgacttcg gtctgtgcga
    18061 actggatcgc cgaggccgcg cacttcgcgc cctcgctcgc gctgcacgac tacgccggca
    18121 gcgggcgtgc cggccggctc gacgggctcg gggccggcga cgtgctggtc tgctcgtggg
    18181 cactgctcgc gcgcgatgcc ggggcgctgg cggcggtgag ctggcatacc gccgtgctcg
    18241 acgaggcgca ggcgatgaag aacgcacaga cgcagcgcgc ccgcgccgcc tgcgcgctga
    18301 atgcggattt tcgcgtcgcg ctgtccggca cgccggtcga gaaccacgcc ggcgagatct
    18361 ggagcctgat gcgcttcctg aaccccggcc tgctcggcag ccgcgaacgc tacgccgagc
    18421 gctacgccaa cccgatcgag cgcagcagcg accccgaggc acgcgcacgg ctgcgccggc
    18481 tgctcgcgcc cttcctgctg cggcggctga agtcgcaggt gctcgatgaa ctgccgccgc
    18541 gcatcgagat cgtgcgcagc gtcgagcttt ccgacggcga gcgtgccttc tacgaggcgc
    18601 tgcggcgcga agccctcgcg cgcgcgagcg gtgacggcgg tggcgagaac gagcggcgca
    18661 tgcggctgtt cgccgacctg atgcgcctgc gccgcgcctg ctgccatccg cagctggtcg
    18721 tgccggacag cgatctgccg tcctcgaagc tcgcggcctt cggcgagatc gtcggcgagc
    18781 tgcgcgaaaa ccgtcatcgc gcgctggtgt tcagccagtt cgtcgatcac ctcgcgcacg
    18841 tgcgcgcctg gctcgacgcc gaaggcatcc gctattgcta tctcgacggc agcacgccgg
    18901 ccgccgagcg cagccgccag gtcgccgcct tccagagcgg gcacggcgag ctgttcctga
    18961 tcagcctgcg cgccggcggc accggcctga acctgaccgc cgccgactac gtgatccacc
    19021 tcgatccgtg gtggaacccg gcggtcgagg atcaggcctc ggaccgcgcc catcgcctcg
    19081 gccagacccg gccggtcact gtctaccggc tggtcgcgca ggacacgctc gaagagcgca
    19141 tcgtcgagct gcaccggcgc aagcgcgatc tcgccgagag cctgctcgcc gatgccggcg
    19201 acagccgcct gctgtcggcc gaggacctac tggcgctgct gcgcgagccg tgagtcgtga
    19261 gtcgccgcgg ccggaattcc cttgccggat ggctcaagcc gtgtcgccgg cggccgattc
    19321 cttccccctc acggctgccg gcacggctgg cgatgctgcg gggcaacacg taaacgactg
    19381 aaacgcaacg cggatcgaca cgctggcggc tgatgtgcgt gtttacgctc ctccgacacg
    19441 ccggcacgga cccggaacac ggggaccgtg cggatggctt gacggacgag tccggctgcg
    19501 cgggccggaa cgggtttcaa gggggcaggc atgacgctgg atgatgaacc gggcctcggg
    19561 gccggatttg acgtgctggc cgggctgatc gacgcggtgc cggtcggcgc gctgctggcc
    19621 gatgtccggg gcacgatccg gctcgtcaac cgcgagctgg ccgacacgct cggctacgac
    19681 gccacggagc tggtcggccg tccggtcgag gacctgctgc ccgagacgct gcgtgctgcg
    19741 cacgcgggct ggcgcaccgg cttcctgcag cagccgcagc gccggccgat ggggcagggg
    19801 cgcgcgctgc atgcgcggat gagcgatggc agcgaactgc cggtcgaggt cggcctcagc
    19861 ccgctcgtgc tcggcagcga gcgcttcgtg ctggcgacgc tgaccgatct gaccgcgaac
    19921 cggcaggccg agctgctgtt ccgtcaggtc gtcgacggcg cgccctacgg cgtcgtcgtc
    19981 gtcgacggca gcggctgcat cgtgctcgcc aatccgcagg ccgacgccct gttcggctat
    20041 gccacgcacg aactgctcgg ccgcccgatc gaatccctgc tgcccgagcg ctaccgcagt
    20101 gcccatgccg gcctgcgcga gcagtacggc cgtgaccggc agatgcggcg catgggcgcg
    20161 ggccgggacc tgaccggcct gcaccgcgac ggcagcgagt tcccggtcga ggtcggcctg
    20221 agcccgatca cgctgcacgg cgagacctgc atgctggcgg cgatcaccga catcaccgag
    20281 cgcaagaaga tggaactcga cctgcgcaag accaacgcca atctggagga attcacctac
    20341 gtcgcctcgc acgacctgcg ctcgccgctg cgcggcatca gcgacctgct gtcctgggtg
    20401 cgcgaggacc tcggcgaggc cccggcgccg gcggtcgtga agaacctcga tcgcatcacg
    20461 ctgcgcgtgc agcgcatgga gcagctgatc gacaacctgc tgagctatgc ccgcgccggc
    20521 cgcgccgcca ccgatttccg cgagcttgaa ctcgacccgc tgctcgatcg cgtctgcgag
    20581 ctcgtgcagc tgccgccggg gttcgagctg cgccgcgagc ttgtcgagat ccggctgttc
    20641 agcccgccga cgccgctgga gacggtgctg cgcaacctca tcgccaacgc catcaagcac
    20701 cacgaccgcg agcacggcct ggtccgcatc ggctgcacgg ccgagggcag ctactgccac
    20761 ctgacggtca cggacgacgg gccgggcatc ccgaagccgg cgcaggagcg catcttcaag
    20821 ctgttccaga cgctgaccgc cgccgagcgc ggcggcagcg gcatcgggct ggccgttgcg
    20881 cggcgactgg ccgagaccca cgggggccgg ctggaactga cttccgaccc cgaactgcgc
    20941 cagaccacgt tccacgtctg gtggccccgc ttccagcgcc gagacctgca cgatgacaag
    21001 aatgaatctg ctgctcgtcg atgacgacga tgtcgccacc gaggccgtgc tgcgcagcct
    21061 cgcgaagtgc gacctgaaac cggtcgtggt caccgccgag gatggcctcg aagccctgca
    21121 cgtgctgcgc ggcacgcacg cgggcaagcg catcgaccgg ccgtatctgg tgctgctcga
    21181 cctgaacatg ccgcgcatga acggtttcga gttcctcgaa aacctgcgcg ccgacgagcg
    21241 gttgcgcgac accgtcgtgt tcgtgctgac gacctccgat gccgacaccg accgcacgcg
    21301 cgcctaccac gagaacatcg ccggctacat ggtgaagtcc tgcatcggcc cgcagttccg
    21361 gcatctggtc agcctgctcg acgtctacca gcagaccgtg cgcctgccgg gataagccgc
    21421 catgcccgat ttccggattc cgctcccctg gccggagacc actgacaagg cacagctgcg
    21481 gctgctggtg gtcgatgacg acgatgtcga tcgcgagcgc gtgcgccgca tgctgctgcg
    21541 caccgcaccc gatgccaccg tcgtcgaggc ctgcgatccg gaggagacgc tggcgctgct
    21601 gcaggagcag gcgttcgact gcatgatcgt cgactaccgg ctcggccggc tcaccgggct
    21661 cgacctgatg gacctgatcc gcgaggtcgt gccgcagcgc tgcgcgacga tcatggtcac
    21721 cggcctcggc gacgaggaga tcgccggcgc cgccgtgcgc tcgggcatca acgattacct
    21781 gaccaagaac cagctcgatc cggtcgggct gatgcgctcg gtcaccgggg cgatgcgccg
    21841 caccgcgctc gaacgccggc tgcacgaact cgcgtactac gacgcgctga ccgggcttgc
    21901 cagccgcacg ctgctgaccg accgcctgca gcagctgatc gcgcggcgcg cgcgcgagcc
    21961 gggtatgctg gccgcgctcg cctacatcga tctcgatcac ttcaagccga tcaacgaccg
    22021 ccacggccac cacgccggtg atcaggtgct cggcgtcatc gcccagcgcc tgaagggcgc
    22081 gatccgcgcc agcgacaccg ctgcccggct cggcggcgac gagttcgcgc tgctgctgac
    22141 cgaggtcgag tcggccgagg actgcgaggc cgtgctcgag cgcatcagcc ggctgctgcg
    22201 cgagccgatc cggctcgatg gccgcagcga tggccagacc gtgcaggtca ccgccagcat
    22261 cggggtcgtg ctgatccagg acgacagcct cgaggccgac agcgtgctgc gccacgccga
    22321 tcacatgatg tacgaggtca agaacgccgg gcgcgagggc gtgcgcttct tcgatccgca
    22381 ggtcgaacgg cgcctgaagg ccgcgcacga cgagctcgaa gcggtcgccg ccggcctgcg
    22441 caacggcgag ttcgagctgt tctaccagcc gcagatcgac ctcgccacgc gcgcaccgac
    22501 cgggctcgaa gccctgatcc gctggcgcca tccgcagcgc ggcctgctcg cgcccgatgc
    22561 cttctggaac gcgctgcagc acaagaagct gtcggcgacg ctcggcgaat gggtgatcgc
    22621 cagcgcctgc cggcagatcg acgcctggct ggccgccggc ctgcggctga aggtcagcgt
    22681 caacgtctcg gtgcatcacc tgctgcaccc gtcgttcgcg gcccggctcg gcgcgatcct
    22741 cgcggcgacg ccatcggtgc cgcccggcta tctggagctc gaaatcctcg agaccgtcgc
    22801 gatcgatgac ttcgaatcga cggtcgccgt gatcgaggcc tgcaaggcgc tcggcgtcag
    22861 cgtcgcgctc gatgacttcg gcaccggcta ctcgtcactg acgcacctgc gcagcctgcc
    22921 gctcgacgcg ctgaagatcg accgcagctt catcgcctcg atgctcgaca gtccgagcga
    22981 tcaggccatc gtgctcggca tcctcggtct ctgccgcgcc ttcaaccggc gcgcgatcgc
    23041 cgagggcgtc gaatccgagg cgcacatccg ccgcctcgtc gaactcggct gccactgcgg
    23101 ccagggctac ggcatcgccc ggccgatgcc ggcggccgag gtcgccggct ggatcgagcg
    23161 ccagcgcgcc tgcgtcgcgg cctgagcgta cgcggccgct tcagcggtgc cggaggcgcc
    23221 ggcggccgat caggccgagc ccgccggccg ccagcgccag actggccggc tccggcaccg
    23281 tcaccagcgc gttggcaagc gccggatcgg cgttgccgtc gggcgcgaag tcggtgatct
    23341 ggttgttgtt gccgctgccg acgcgcggcc acaggttcca cgtgtaggcc gcgggcgcga
    23401 agccggccga gggcagcgcc gacagcggca cgtcgacgct cagcgtgtca ccgacgaggc
    23461 tcgcgctcag cgtcgcggtg acggccacac ccggcgccag cgtgcgcacg gtggcggtgc
    23521 cgagcgcatc gatgaccacg accgagtcga acagcacgcc gacaccgccc ggcagcgtcg
    23581 cgaagcgctc ggtgccggcg ccgcgatcga agccccagac gtacagcgcg cccggcgtct
    23641 gcccgagcgt gtcggccagc gtcgcgctca ggtggaaacc gctgccgttg aagatgacct
    23701 cggcgaacag cacatcgaca tcgccctgcg ccggcacgcc ggcataggtc gacaggatgt
    23761 cgccggccgg gtcggtgacc ggcacggcat gggcgggagc ggcggacagc gcggcgagca
    23821 gcgtggcggc gagcggcgcg agcagcgagg cgacgggcgg acggcggtgc atggggtgtt
    23881 ctcccttcgg tcaggaaccg cgaggctgcg gctcgccggg ggctacaccg gcaccggtgc
    23941 cgggcttcca ttgccattcg ttcagcttgc ccggtcgcgc ggatggcagc ggccagactg
    24001 cagcgaccgg ccggcccgat ccggggccgg ccgcagtacg gagtcccgcc ggtggccgaa
    24061 gccgcgtccg ccccgtccgc atctgccgcc ggtgcccgcc gcgccggctg gttgcgccag
    24121 tcgcgcttct ggctgctgct ggccgctgcg ctgctcctcg ccggctcgac cgggcttgcg
    24181 ctgcatcagc cgccgcaccc ggatgcctgg cgcccgccac cgggctggct cgatccggcg
    24241 ttctggcgct ggccgttcga gcgcaacgcc ttcatgcgtc tgccgctgct cgcggccccg
    24301 atcaacgcgc tgcatgtcgc cgacgatggc acgacctggc tgctgaccgg ggctggcgag
    24361 atcctgcaca tgtccggcga cggcctgcgc tggcagcggc agtggccggt ggcgacgcaa
    24421 gcgccggacg cccgcgcggc aggcagcgcc atcccgggca tcgccgttgc gcacgcccag
    24481 gccatgccac cgctggacgg cgcgatcggc aagggcagcg aggtgccgaa ccaggctgcg
    24541 ccgcaatccc cggccgctcc ggcctatccg aagcccgggc cgaccgcgcc ggtgcagcag
    24601 acaccgatga cgggcgaagc cccgcagccg cgcgcaccgg cattgaacgc cctgcacttc
    24661 ctgagcgacg ggcgccacgg ctgggcggtc ggtgacgccg gcacgctgct cgtgaccgac
    24721 gatggcggcg agcactggca gcggatcgaa ctcgccgagg ccggcgaaaa ggtgctgcgc
    24781 agcatctggt tcgtcgatgc gcaatacggc tggatcgtcg gcgacgacgg cctgcgcctg
    24841 cgcacgatcg acggcggcag gagctggcag ccgcccggcc cgacgcgcgc gctgccgctg
    24901 ccgagactga atgccgtgcg cttcatcgat cccctgcacg gctgggcggc cggtgagcag
    24961 ggcacgctgc tcgcgagcgt cgacggtggg gcgagctggc tcacgctgac gaccggcacg
    25021 caggcgagcc tgaccgcgat cgacgtggtg gctggtggcg accggatcag cgtcgtcggg
    25081 ctcgaaggta cgctgctgca gttgagcctg gccaaaccgt tcgcgccgca gccggtgaag
    25141 ctcggcggca tgaatgcact cgatgtgcgc ttcgccgccg acggccgtcg cggctggatc
    25201 ggcgctcagg acggctggct gctggtgacc gacgacggcg gccagcgctg gcagcggcag
    25261 cggctcggtg aggacaacct gactgcgctc gccgtgtccg ccgacggccg gcagcagcgg
    25321 gtcggcgctc taagcggcgt gctgcaggcc cggctcgacg atgcccgctt cgtggcgctg
    25381 acccgcggcg gcgaacggca ggcgcgccgg ctggcgggca gccgtgacgg ccggctgttc
    25441 ctgctcgggc cgtacggcga tgtgctcgtc accgatgcca ccagcggcaa ctggcagcca
    25501 ctcgtgaccg gcagcggaca caccctgctc gatgtgtgcc ttgccgccga cggcttgcgt
    25561 ggctggctgg tcggcacgtc cggcaccttg ctcgcgaccg ccgacggggg gcgccactgg
    25621 cagccgctgg caagcggcac cgggcagaat ttgctggcgc tcgactgcag tgccgatggc
    25681 cggcgggtcg tggcggccgg ccagtatggc gttcttcttg ccagtgcgga cggaggcgtg
    25741 aactggcagg tgcaggcgca ggggatgtcc gatctggtcg cggtgcgcct gacagcggac
    25801 aagctgcagg ctacggcgac cagctcggac ggccgcacgc tgacaatgac cgatggcggc
    25861 gcggactgga cggccggggg ccgcgcacat gaaggtctga tgctgcacac cgtgcgcacg
    25921 gcggccgatg tccgccgccg ctgggcgatc gacctcggcg ggcggttgtg gagcactgcg
    25981 gacgccggtg cagactggca gagggttgcc ggcctgcccg agggcacgcc ggccctgtac
    26041 gacgtggaag tgctgcccga tggccggcgc ctgtttgtcg tcggggtgga cggcacggtc
    26101 ctgcgcagcc tcgacggcgg cacgacctgg cagaagcccg acgtcgnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnntcgc tgatgaacct gctgcgcggc gaactggtgc
    26281 gctacggcgt gtgcgcggtc ttggcaccac cagaaggaag aacagctgct cgcggcgctg
    26341 gtcgacggca tccgccggca ggcgatcccg tcgccgctcg gcttcgacgg cctgcgcttt
    26401 cgcgcacggc tgctgtggct gcgctctggt cagcactggc cgtgggcgct gctgttcgtg
    26461 ctgctcgcct gctggtccgg ggcctacttc ctgcaggact tcccgctgcg cttcgagcgg
    26521 gcctgggcga tcacgcagca ggtcgttgcc ggcatccggg cccgggagtc gcaggccgat
    26581 ccgccgaagt ccggaccggc gaaatcatcg gaagacccct ccgggcttgc cgctgcgaag
    26641 gccgccggca acccgccgga ctccggggcc gggacgacct cgacaaatgc gtctgccggt
    26701 tcggggcagg atgcaggtgc cgggcaggga agggcggttg caggcgttgc gacagcctct
    26761 gttgcgcctg ctccggaggc ggagcctgcc gtgcggccgg ccgcgttcgc tcaggggctg
    26821 gcggcgctga tcgctgcgct gacggcgctg acggcggcgg tcagggccgt gcgcgccttc
    26881 ggcgtcaggc ccgaagcctt gcttgcgagc ctcgcgacca agacccgcgt cagcgatctc
    26941 ggcgcgcaga cgagcttccg ctaccgctac cggcaggagt tcgccgaggt cacgcaggcg
    27001 ctcgcgccgc gcacgatgct gatcctgatc gacgacctcg atcgctgcca gccgacgcag
    27061 gtgctggaga tgctggaggt cgtcaacttc ctcgtctcgg ccggcgactg cttcatcgtg
    27121 ctcggcatcg acaaggaaaa ggtgcagggc gccctcgggc tggcgttccg ggagctggcg
    27181 caggagatgg tcgatcaggg cagcgatgcg gcggataccc gcagcgacga ggcccgcgcc
    27241 cgcgacaagc gccagcgcta cgcgcgcgat tacctcgaaa aactcatcaa cctcgaggtg
    27301 ccggtgccgc tgctcgacgg caccgatccg gcgcgtggcc gcggcatgct cgccggcgag
    27361 tcggctgcgc gggaacaggc cggactgcgc ctgcaccgct ggcgcgaacg gctgggcagc
    27421 ggcctgcgcg tgcttgccgg cctcttcctg ttcatcgcgg cggcagccgg cggactgtat
    27481 ctggccggcc tgacaccggc gctgccgccg ccggcgacgg tcggcccggc gacgacgaca
    27541 ccggccggca gctccggtgc ggccggcggc gatacccgcc cggtcccggc cggatcgtct
    27601 gcgccgatgc ctgcggcgca aggcgctgcc agcgtgatcg gccagcaggc cggcttgccg
    27661 tggccgctgc tggtcgcggt gccggcgctg gcgctgctgc tcggggggct ggtagccgcc
    27721 ctgcgccggc cggacatcgt cgtgcgcgat tcaccgcgtt tcggcgaggc gctcgacatc
    27781 tggtacccgt atctgctcag ccaccaccgg ccgacgcccc ggacgctgaa gcgctaccag
    27841 aaccgcatcc gctacgtcgc gatgcgccag cgcccggccg agcctgcgac cgatccgctg
    27901 gagcgcctgc tgcgccgctg gctgggggtg ccggccgagc cggactcggc ggatgacgcc
    27961 ctgccggagc cgctgctggt ggcgctcggg gcgctgcagc tggtcgatcc gcaggctctg
    28021 gtcgcggagg caggggcgct cacgccggcg gacgcgacga agggggccgc tgcggccgga
    28081 agcctggcgc aggcccgtgc cctcgccgaa gccgtcgaac gccaccgcgc gcacttcggc
    28141 gacgcgagct ggccgccgtc gcccgcggcc gtgcgccgct tcctgaagct cagcgaaggt
    28201 gtggcgatcc gttgatgcgg gccggccgtc gcccgccggt tgcgttccgg ccgccggctt
    28261 gacgtgtccc cggggtgtcg ccatggtctg cacccgtgtc gacacgtaca tccacggagg
    28321 cgaccatggc gaccacgacc cgcgtttttc gcaacggcaa tgcgcaggcc atccgcattc
    28381 cagccgagct ggcctacgag cgcactgacc tcgaactgga aatcgagcgg atcggggacg
    28441 agttgcgcat ccgtcccgcc cgtcgccggc tcgacggcgt gctcgcccgc ttcgctgcct
    28501 tctcgcccga tttcatggct gacgggcggg gcgagaacga ggaagccgaa cgggacggct
    28561 ggtgatatgc cgcgcttcat gctcgatacg aacatctgca tctatctgat gaagcaccat
    28621 ccgcccgaag tcgcggcgcg ttttgccgag tgttacgtcg gcgacgtggt gatgtcggcg
    28681 atcaccctgg ccgaactgca atacggtgtc gtctgctccg gcgagtgtcg cgatcagaac
    28741 gccgcggcgc tggccgcgct gctgggcgac attccggtcg ccgcgttcga tgccggggca
    28801 gccgaggctt acggtgccat ccgtctcgcc acccgcgagc gcaagcgcga tgcgctcgac
    28861 aagctcatcg ccgcccatgc gcttgcgctt gcagtcacgc tcgtcaccaa caacgagcgc
    28921 gatttcgccg attacccggg attgcagatc gaaaattggg ttggaagccg ttgagtcgcg
    28981 tagggcgtgc gacttgctac gcccgctccg gcgcttcgcc gagccattcc tctgcgcgtc
    29041 cggatacgtc actgcgtccg ctgcggtagg cgccgacgcc gggcagcttg cggacggtac
    29101 ccgggtttcg gcgttcggca tattcgccca gcgcttcgcg gatcagttcc ggcaccggtc
    29161 gctgccggtg ctcggccatc tgcttcagca acagggcgag ctgttcgtcg aggatagcgt
    29221 cgtgcggtgc atggggttgc tccggtcgcg gtgcctgccg accgcgcagg ggatgacgat
    29281 ccatgcagcc gcaggctcac ccccgtcgca accagcccgt gacctcctcg cggtcgtgat
    29341 agagctgctt cagcgccagc cggtacgggc tgccggccag ggtttcggcg atcagcgcct
    29401 cgcagcgctg cagttcggcc cagcgcttct tcatcggcag cttcaggttg aagatcgcct
    29461 gcttcgcggc gccatcggcg atccagccgg cgacgagctg cgcgatgcgc gccggctgct
    29521 cgaccatgtc gcagaccagc cagtcgaccg gctcgcgcgg gcggaagcgg aagccgtcct
    29581 cgcgccggtg ctcgacgagg cccgaggcca tcagggtttc gtccatcggg ccgttgtcga
    29641 tcgccgcgac gcgcaggccg cggcgcacga gctgccaggt ccagccgccc ggcgcggcgc
    29701 cgagatcgac cgccgtcatg ccgggacgca gccggcgttc gcgttcatcg ggcggcagga
    29761 agacgtggaa ggcttcttcg agcttcagcg tcgagcggct cggcgcgccg ctcgggaagc
    29821 gcagccgcgg gatgcccatc ggccagtccg agctgttgcc gggctgcgag accccggccc
    29881 agccggccgc cgatgacagg aagaacaggt gcaggcgcgg cgcgcgcgcg tcgtcgacca
    29941 gcagcccggc cttgcgcgcg gcggcggcga aggccttctc gaacggtcgg cagaaggccg
    30001 acagcgcctt ggcctcgttg gtgtccggcg tctcgacgcg cagcgccccg aacgggccgt
    30061 cggcgagtcc gcgcgcggcc tccaggagcg gcgtgacgcg atcatcgacc ggcagctcgc
    30121 gcagcagctc gccacaggcg atccactggc gcgcgaagat cagcgcgctg aaatcgaggg
    30181 cggcggcgaa cgcgcgggcg tcggtgccgt agggctggaa cgcgacatag ccggaatcgg
    30241 gcttcgcctt cgcgaaaccg gcgaagccgg cttcggccgc gcgggtctgg atctcggctg
    30301 cgcattcgcc ctcgaagccg gcgcggcagc acagcagcag cgtcgtggcg ctggtcgtgt
    30361 cggtcatggc ggcgctcagt cgccgacttc gatcagcggc gcgccgatca gcgcttccag
    30421 gccggcgacg aactccggcg cgagcggcgg ccagttccag aagctcaggc ccaggcagcc
    30481 gaagccgacc gcgggctcgc acggccagct cgtgaccggc gcgagcagcg tgcagccggt
    30541 gcagagcagc aggccctcgt cggcgtcctc gccgatcagc agccactgtt cgacggcatc
    30601 gaaccagcgc gggtcgccgg cgatcgccag cggcttgccg cagtgcggac agccggcgct
    30661 gccgagcgcg ctgccgtggt agaccatgcg cccgctgcgc acgagcacgc cgttgaccgc
    30721 cagccgctgc aggcgctcga cggcgcgcgc atcccgcggc tcggccaggg cctgcgcgca
    30781 gtgcgggccg ggcgcgtagc cggcggcggc ctccggcagg caggcggatt cgacgccggc
    30841 caggatgccg ccggcctgca agcgtgcgag cacggcggca gcacggtcgg cgccgagggc
    30901 cgccggcagc tccggggcga cgagatggcg gacgcgatcg ggcatgggcg gacgggcctc
    30961 aggtgccggc ggccgtggcg cggcgatgct tcacgtactc gatgacgccc ggcagcagcg
    31021 acacgacgat gatgccgagg atcaccagcg tgaagttgtt cttgaccacc gggatgttgc
    31081 cgaacaggta gccggcgtag acgaagctgc cgacccagca cagcgcgccg agcgcgcaga
    31141 agccggtgaa gcgccggtac tccatgctgc cggccccggc cacgaacggc gcgaaggtgc
    31201 gcacgatcgg tgcgaagcgc gcgagcagca cggtcttgcc gccgtgcttc tcgtagaagg
    31261 cgtgggtctt gtcgaggtag gcggtcttca gcacgcgtgc atcgggccgg aagaccttct
    31321 cgccgaggaa gtggccgatc cagtagttgg tgttgtcgcc gaggaaggac gcgagcatca
    31381 gcagcaggaa caccgcatgc acgtcgagcg cgccgcccgc cgccagcgcg ccggttgcga
    31441 acagcagcga atcaccgggc aggaacgggg tcacgaccag ccccgtctcg cagaagatga
    31501 tcgcgaacag gatcgcgtac agccacgggc cgtagtcggc ggcgaggatc gcgaggtgct
    31561 tgtcgaggtg caggacgatg tcgagaaact gggcgagtaa ttccatgcgc gggtccggag
    31621 cggaagcgga acatccatgc tacctgccgt gtgcgctgca gcacaggcac cggccgggcc
    31681 gcgggcgtgc ggccggggtc tggcggtgcc ggcggcggct tacgatgcgg tttcaggcgg
    31741 acccggcacg gctgccggtg acggttcggg gaggccgggg tagggcgggc ggttgtccgg
    31801 gtcgctgcgc agggcttcgg agaaggccag ccgctggccg atgtcggcat cgtccaccgg
    31861 cccggggctg gcgcggtggc ggtcgcggct gatctcggcg ccggtgcggc ggtcgatcag
    31921 ggccagctcc acgggctcgc cggtgtcggt gtcgaccagc tgcacccggc agccgtccgg
    31981 cgcgtagtgc cggttgccga aatcgagcag cgtcagcagc accggccgga aatcctcgcc
    32041 gcggcgggtc agcaggtatt cgtggcgggg cgggcgctgg cagtagcggt gcttttcgag
    32101 caggccggcc tcgaccagat gcgccagccg ccgcgccagc atgttcggag cgatgccgag
    32161 gctcttctgg aactcgtcga agcggctcag gcccaggaat gcgtcgcgca ggatcagcat
    32221 gctccaccat tcgccgaccc gttcgaggcc gcgggcgatc gggcaggaca tggcgccgaa
    32281 atcggtgcgc tgcatgggaa gtgggctcgg gcggcggcgg tcggccggtg actttatggc
    32341 cgctgccgcc cggcgggcaa ggctgcggcc gggatgtttt tgtgtatgat ccaccggctt
    32401 tcgccgcacc cggcggtgaa ccgacgcgaa agtggcgaaa ctggtagacg cgctggattt
    32461 aggttccagt ggggcaaccc gtgagagttc gagtctctcc tttcgcacca gtgtccagcg
    32521 aggtccgcgc gcgcccgtgc acaggcgatg cgacgcgggc cttttcgctt cgttccggat
    32581 gatcaggggt acccagatgc aggtttcagt tgaggagctc ggcggtctcg agcgtcggct
    32641 cacggtgcag cttcccgcgg ccaggatcga gcaggagatc gaacagcgcc tgcaggatct
    32701 ctcacgccgc gtgaagctcg acggcttccg tcccggcaag gtgccgctga aggtcgtcaa
    32761 gcgcatgtac ggcggccagg tccggcagga agtcctgggt gagctgatgc agtcgagctt
    32821 ccaggaagcc gtcagcggcc agaacctgcg cccggccgcc ggcccgcaga tttcgccgtc
    32881 cgcagccgcc gaaggtgact tcgcctatgc ggcgaccttc gaggtcctgc ccgaattcga
    32941 gctgcagggc ttcgaaggca ttgccgtcga gcgcccggtc gccgaggtca gcgacgccga
    33001 catcgactcg atgctcgaaa ccctgcgcaa gcagcgcacc gtgtggacac cggtcgagcg
    33061 cccggcgcag accggcgatc gcgtcaccgt cagcttcgag ggcacgatcg acggcgtcgc
    33121 cttcccaggc ggcaagggcg atgacgtgcc ggtcgtgctc ggggccggcg gcatgctgcc
    33181 ggacttcgag aacggcctga tcggcattgc ggccggcgag gcgcgcgaga tcgccgtgag
    33241 ctttcccgag ggctatcacg cgcaggaact cgccggcagg acggcgcgct tcgcgctgaa
    33301 tgccggtgtc gtcgccgagc cgagcctgcc gccggtcgac gaggagttcg cgaaggcctt
    33361 cggcgtcgag gacggcagcg tcgagtcgct gcgtgcggcg ctgcgcgaca acatgacgcg
    33421 cgaactgggc cagggcatca agacccgcgt caagaagcag gtcatggacg cactgctggc
    33481 cgcgaacccg atcgaactgc cgaaggtgct ggtcaccgag gagatcgacc gcatcgcgca
    33541 gcaggccggc ttcccggcgg ccagtgccgg caacgccgag gccgacaaga tcaaggaggg
    33601 ggtgttcgcc gaggaggccc gccggcgcgt cgcgctcggc ctgctgatct cgaagctggt
    33661 cgtgcaggag aagctgaccg ccgatccggc gcgcgtcgtc gaacagctga cgacgatggc
    33721 ctcgacctat caggacccga acgaggtcat gcagtggtac cttaagaatc cgcaggcgat
    33781 ggaaggcctg cgtgcgctgg cgctggagga caccgtggtc gactgggtgc tcgccagggc
    33841 cagcgtcacc gagcagccca gcactttcga tgcggtgatg cgcccggccc cggcaccggc
    33901 cgccaccgcc gacaacgccg cctgacccgc ggcaggagac aggtgacatg tacccgagca
    33961 agggcggttc cgacatccag gcactgaatc tggtgccgat ggtggtcgag cagtcggcac
    34021 gcggcgaacg cgcgtacgac atctactcgc gcctgctgaa ggagcgcgtg atcttcctgg
    34081 tcgggccggt cgaggattac atggccaacc tcgtggtggc gcagctgctg ttcctcgaag
    34141 ccgagaatcc ggacaaggac atccacctgt acatcaactc gcccggcggc tcggtcagtg
    34201 ccggcatggc gatctacgac accatgcagt tcatccggcc ccaggtcagc acgatgtgcg
    34261 tcggccaggc ggccagcatg ggggcgctgc tgctcgccgg cggcgccaag ggcaagcgtt
    34321 actgcctgcc gcactcgcgg gtcatgatcc accagccgct cggcggcttc agcggccagg
    34381 cctcggacat cgacatccac gcccgcgaga tcctgctgat ccgtgaccgc ctgaaccgcg
    34441 tgctcgccaa gcacaccggc cagccgctcg aacgcatcgc ccaggacacc gaccgtgaca
    34501 atttcatggg cggtgaggca gcggtcgaat acgggctgat cgattcggtg cttagccagc
    34561 gcagcgctcc ggccaccgcc tgactcctgc accgacagcc cgcccccggt ccggctccct
    34621 ccggccgggg gcggtgcgtt tccttgccat gaccggacgg gcgtagttga atacgaccga
    34681 acgggctggt ccccttcagg tcacattcct gctgtttcca tccgcacggc gcactgtctg
    34741 cgccgggcct ccgtaccggc acggggccga tcgcggcgca catcagggcc gcccaccgcg
    34801 tgaggatcgc accatgagca aagaccgaaa cgacaagggc gatgacggca agctcttgta
    34861 ctgctcgttc tgtggcaaga gccagcacga agtccgcaag ctgatcgccg gtcccaacgt
    34921 cttcatctgc gacgaatgcg tcgagctctg caacgacatc atccgcgagg agatggagga
    34981 gaagacgccg agcgccggca gcaagctgcc gaagccgcac gagatcaaga aggtcctcga
    35041 cgagtacgtg atcggccagg accaggccaa gaaggtcctg tcggtcgccg tctacaacca
    35101 ctacaagcgc ctcgaactgc gccgtggcaa ggacgaggtc gaggtctcca agtccaacat
    35161 cctgctgatc ggcccgaccg gttccggcaa gacgctgctc gccgagacgc tggcgcggct
    35221 gctgaacgtg ccgttcacga tcgccgatgc gacgacgctg accgaagccg gctacgtcgg
    35281 cgaggacgtc gag
//
LOCUS       DAOQIE010000002        34592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_503_length_34592_cov_7.379622, whole genome shotgun sequence.
ACCESSION   DAOQIE010000002 DAOQIE010000000
VERSION     DAOQIE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 34592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34592
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_503_length_34592_cov_7.379622"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..2859)
                     /locus_tag="PK144_00185"
     CDS             complement(40..2859)
                     /locus_tag="PK144_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNF64168.1"
                     /translation="MIFTRLFRRAPSWRHRDPRERLRALQSLADGDPALLELAGDSDP
                     GVRRAALDRLHDPLRLLEHLHAEDDAVVREAATGRLRTLLAGHLAGGPAVTVRLAVLA
                     DPRLPPALLVHLAAEAAESALRLAALAGVLDQRQLAVIAVQDAVAEVRLAATERIHDP
                     ELLAGLARSFRARDKRLYRLVSERLETVRRRETERLRAAALLDELDALAARERLEAED
                     VVRAAALEREFGQLPAEADDPARRQAAQARLAPLLAAHQAHGVAVARCLRALDAITAG
                     FAAHGDATACATALAGLEAEAPADPRIVVRMQALRERLAERGRDTAHAEARERWLNAL
                     ETLASIDAATRARLEAEWQALPAPADATLRRRQDERARARLAQLLAAQPAPAPTQSSA
                     PKPAAPRRAPAEPDAQLLARLETLEAALEAGELHTAQPLYEQIQTEARGLRALDARLH
                     AAAARLAELRDWARWGGRQAREQLCLAAEGLVPDAWAPAELARRVQQLRAQWKQLDGG
                     ELPATRGQWERFNSACERAYAPAQAAFAQAAAEREQHIGARRALIERLQQLDAGTDWS
                     APDWRTLDQAWRELQRSWREAGTVPRNAYRTLEQDWKAAAEAVGGRIEAERQRERARR
                     EALIVQLQSLADEDLRTAIAAARQAQADWSPTLQLRRDAEQALWLRFRAVCDAIFGRR
                     EQVRSAGQAQRQATLDAAAALCAELETLAATPLDADNAGAARAAAARIAEDWAGLGEL
                     PRAAQHAIEQRYAAALDAWHERLTGLERVQRRKAVHALAEKATLCARLERHVLDGPPA
                     TAGDPGADGADDEPAALREEWQRLARLPEPLERRIRSRLDAAQRALADPVEAHRLAMR
                     MTEGASIRHRLCLELEILAGIEPPPEDAQELLEHRIARLSAALSGEAPPDADSVIHDW
                     YCTPAAADPALDTRFATALVALGQA"
     gene            complement(2968..3756)
                     /locus_tag="PK144_00190"
     CDS             complement(2968..3756)
                     /locus_tag="PK144_00190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF64169.1"
                     /translation="MDGGWLRPLLFVGVLLALLALESRAPRRAWTQPRWPRVARHLAL
                     VGLDAVLLRLTLPLLATGAAALAEARGWGLLRALAVPAPLAGMLSLLLLDLAIWAQHV
                     AMHRVPLLWRLHRVHHTDTDFDTSTALRFHPLEIGLSMLWKIVLVLALGAPVAAVAGF
                     ELLLSACALFNHANLALPAPVDAWLRRLLITPDVHRIHHSIHRDETDSNFGFSVVWWD
                     HLFGTWRSAPRDGHATMTIGLDGFRAPQAQRLGALLLQPLRRDG"
     gene            complement(3760..6534)
                     /locus_tag="PK144_00195"
     CDS             complement(3760..6534)
                     /locus_tag="PK144_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNF64170.1"
                     /translation="MPLLLSLFLIILGAITGAEATRDDAAGAVLGATLGLTLGLLLAL
                     RRRVQRLEARLDAPGTGQPAPAPAEAVRPAAVPARETVWTRAAVEPAAVAQAAAPPPD
                     GWQPGFPQAAIPPGWDTPQSGAAGASLRSRLQDWLAGGNALVRTGIVVLFFGAAFLVR
                     YVAEHSQLSIELRLAALAATGIAGVALGWRLRERRRGYALSLQGGGLGLVYLVLYGAM
                     QFYGLIPPLAGFALLFACTAAGVALAVAQAAEVLGLFAVLGGFLAPVLVSTGSNDHVA
                     LFGYYLVLDLGILALLLREPWRRLAVLGFVCTYVIGTLWGGLRYRPELLASTEPFLLA
                     FFGLFSLLPVLAAHRLPPDPKRPLDAGLVFGVPVLTLALQAALLEGVEHGLALSALGC
                     AAWYAALWRFCRQRPALALLGECQAAIALVSATLAVPLYFEAGTSSPLWALEGAGLVW
                     VGARQDRRGLTGFGALLLFAAGALWLDAHLPDMPVSRPFLRADALDTPLLALAALVAA
                     AALGRLRPAPQPLRLETVLAGWGAFWWLLWCLRETAMSTLAVDAASGVWLLFLAASSA
                     LAFLAWRRLALGWLRVPLYGLPLLLAPLAMHDFLHRDHPLAGLGAAGWLAAFAVQFGL
                     LRVESSAADAGDRGQRWRYAAAVWLLLALALQEVAWLIGDVVHLAFVWTRAANGVLIA
                     ATLALLLDGERPAWPLAAYGTAGRRLAGAPVALLLLAWFPAAGLHGPALPAPLPFVPL
                     LNPVELAAVAALLVLARAGGRPWFVPPAQRPAARTVLAALGFATLNLAVLRACHHLAG
                     VPWSAAALWDSRLVQTALALVWATAAVVLMLTGTRRGQRGIWLAGAGLMALVVLKLFI
                     VDLDGRDTLARILAFLGTGGLLLLVGWLAPVPPRAAPGPSVDTDTDDDAESGPSPTPA
                     AAPVDAPPAS"
     gene            6696..7706
                     /gene="hemB"
                     /locus_tag="PK144_00200"
     CDS             6696..7706
                     /gene="hemB"
                     /locus_tag="PK144_00200"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNF64171.1"
                     /translation="MPQTFPSRHPAARPRRLRRDEFSRRLVRETVLTTDDLIQPLFVI
                     EGHGRREAVASMPGVERVTIDELLREAEQLVALGVPAVALFPVTAPEAKSLDAASAYD
                     PAGLAQRAVRALKERFPELGVITDIALDPYTSHGQDGLVDADGYVMNDETIEVLVKQA
                     LSHAEAGADVVAPSDMMDGRIGAIRNALEAAGHIHTRILAYSAKYASSFYGPFRDAVG
                     SAGALGKGNKYTYQMDPANSDEALREVALDLDEGADMVMVKPGMPYLDIVRRVKSEFG
                     VPTFVYQVSGEYAMQMAAIQNGWLAEAVIAESLLCIKRAGADAVLTYFAKRVAADLRE
                     RG"
     gene            7706..8374
                     /locus_tag="PK144_00205"
     CDS             7706..8374
                     /locus_tag="PK144_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64172.1"
                     /translation="MRRPGLWIGAAALVLGVLTVPLFAPGSKPSVPGTDLPWQVETDA
                     HGVPAVFGIRLGETRLQDVLPRLGRSVELGLFRQADGRSVLEAYYREIELGGLTTKLI
                     LRLGAGQEALDAMIARAGKGDPTPSGSMQYIPASTDDHAIIALPIIGLTYSPLVRLDE
                     DVIRSRFGEPAEKLAEKDGVHWLYPARSLDLFRQDNGKSILTYLLPADYAQWRAQYRP
                     PSGQ"
     gene            9424..9993
                     /locus_tag="PK144_00210"
     CDS             9424..9993
                     /locus_tag="PK144_00210"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNF64173.1"
                     /translation="MNATHCPRIRQDGFTLVELMVVLSVAAILTTIGVPSFLSTIRDN
                     GVSTRTNELITDLALARTEAIRRGQTVTVCPSTDGTSCAGSGGWEQGWISFRDNDASG
                     VLDSSDCSTPADCVLRRSPALASGYTLRANNVTLAAHLSFDGIGMATASFGQLRLCDE
                     RGISHGRIIDVAAAGRAVAKQPSGSDSCE"
     gene            10075..10530
                     /gene="pilV"
                     /locus_tag="PK144_00215"
     CDS             10075..10530
                     /gene="pilV"
                     /locus_tag="PK144_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNF64174.1"
                     /translation="MDGRRQVQGFTLIEVLVALLIAAIGLLGMAALHLSGLRSVETAY
                     QRSLATLAANDLLERMRANADAVRAGNYASAYGAAATASTDCEATSCSGAQMAAFDLA
                     RWKASLGALPAGDGKVEMSGTRVTVTVRWDHSRSGDGTQWASFLMTTDF"
     gene            10497..11552
                     /locus_tag="PK144_00220"
     CDS             10497..11552
                     /locus_tag="PK144_00220"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNF64175.1"
                     /translation="MGELPDDHRLLNTRAPRCAAGGFTLVEMMLALALGLLIVAGALA
                     VYAANRAVYQHQEAWARLQENARLAFELMARDGRQAGFMACLPVAAPLTNVLNSATTY
                     EWLFEQPVDGFEASGVSWSPTLPSGFPAPYRGTDVLTLRGAQSSPQARVSAQASTSAD
                     VTLDNGSGFASGDFVLLAAADCSEGAIFQVSGVTAGAAPVASLAHAAGAGSPGNASAD
                     LGARYVGGLALKLTTYSYYVAAGSDGRPALYRRQGSATPVELIPGVDDLQVLYGLADG
                     SNGDYASRYATAAVISADADNWQKIVGLRVTMTLESPDDGITDSAQAVTINGVSGSDR
                     RLRRTFTATIAIRNRLP"
     gene            11549..12088
                     /locus_tag="PK144_00225"
     CDS             11549..12088
                     /locus_tag="PK144_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HNF64176.1"
                     /translation="MSATPFCRPPASLRAPRRERGAVLIMTLILLVVATLIALSAQRN
                     TALEERMAGNLLDRNLSFQAAEAALRGGESALANAVAAGSVPVLCSADAGNCAAGWYT
                     AASGAQTPVWKRVGWGDGNSVPIAVAPPSGSTSPLAANPRYIVEQLDASTSTWRVTAR
                     ATGGSGAGVVVLQSIYITE"
     gene            12136..>12572
                     /locus_tag="PK144_00230"
     CDS             12136..>12572
                     /locus_tag="PK144_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64177.1"
                     /translation="MSVRCIRPPKPSSLCVLLLLFAGLLLPGLAAALNLAQAPIYQAP
                     PRKPLVLLSMSKDHTLFYKAYDDYSDLDSDGVADTTYRNAITYYGYFDSLKCYSYDTT
                     NQRFEPAAASSDHYCSGTTWSGNFLNWVSMARIDVVRKVLYGGR"
     assembly_gap    12573..12672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12673..16485
                     /locus_tag="PK144_00235"
     CDS             <12673..16485
                     /locus_tag="PK144_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNF64178.1"
                     /translation="TYVTTATTTSTVTPGIVVLERSYLPNDAHSWSKVYAPSGTSDIG
                     RLTPLGARASISICNTTYAASGSYSQRVDTAAYPPVMRFAAGEFPDWAAQEVRQCQTG
                     VSGSPGSSALIGDYAARVRVCVPGSGTGGLGGEKCKQYPDGSYRPVGVLQDYGDDDQM
                     YFGLMLGTYGGNKSGGALRKNIASWRGDEINLQTGQFLATPSTGSIRAALDALRLFGY
                     KHGGSYTDSYNSGNGGDACGLASNTDPLNSFTEGNCSTWGNPIAELTLEAMRYFAGLR
                     YSTSSTANGLSPTTAFNKDDSARFADLKLVSWQNPASSDQYKCSPASLIAINSGVSSY
                     DSDQLGGFSDLGISGTLSGYTDAIGAAEGISSSGTPLFVGEFGSSTDRRCTAKTVTAL
                     SGVKGPCPEAPRLSGSYQIAGLANAAHTAGVGGRKVDFFGVSMAPPVPKITIPVPAST
                     RTVTILPACKDSNNDSACTIVDFRILSQTATAGQYAGRVSVVWEDGEQGNDYDEDMKG
                     TIDYVVTSNTVTVTTWIDYANAGHTLGFGYIISGTSQDGFHAHSGHNTFRYTDATGVR
                     DCSAGCSVSSPAVQTAQTYTISGTAAGTLRAPLYYAAKWGGFRDRNSNGRPDDGEWED
                     HFFDVKNPGELSPALSRLFASVAAGDPSSAALSANVRTLQTSALLFQASYNPADWSGE
                     VKAYGIDAATGALSGLIWKASDHLPATASRNIVTWRPGSGGIAFTWTNLDSTQKAALG
                     NDSTLLDWLRGDQSREGTASNQLRVRTTRLGDVVNSDPVFAGVSDAGYGRLPGSEGAS
                     YAAWVTAKESLAQMVYVGANDGMLHGFDATTGSERLAYVPNAVFGRLAGLTARSYSHV
                     YSVDGAPAVGDVHDGSRWRTLLAGTLGAGGKAVFGLDVSDPATFGPGNVLWEFTDADL
                     GEGVRQALIVRTHDSAHPWVVLFGNGYNGTSGNAVLYALDALSGVQLAKIVLPVSGAS
                     GLATPVAWDADGDLVTDAVYAGDLLGNLWKFDLSGSAGGWRVALGGQPLFTARGPTGL
                     AQPITVQPTVGKGKDGVVRVFFGTGKYLEASDGQIETSPAVQSFYGLVDAGSSITYTS
                     RSSVLTAQTLIAEFPAGTNGPYPLRVSSDNTPADGAKGWYLDLVPPNGVAAGERVVER
                     AVIRYDRIVFPTMLPLASAGCSGGGDGWLMEVLSASGGRPKTASFDLNNDGRFDGSDG
                     VTINGELVVVTGINPQVGVISGAPAVVVGAKSETKWVTGSAGGTPFSVGERIDARSTG
                     RQSWIQLQ"
     gene            16611..17429
                     /gene="aroE"
                     /locus_tag="PK144_00240"
     CDS             16611..17429
                     /gene="aroE"
                     /locus_tag="PK144_00240"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNF64179.1"
                     /translation="MTDRYAVIGNPIAHSKSPRIHTLFAARTGQDLVYEAILGPQGGF
                     ADCATAFRAAGGRGLNVTVPFKQDAWVWADRLSTRAERAGAVNTLKLDEDGVLGDNTD
                     GAGLVRDLETNLGCTLAGRRVLLLGAGGAARGVLAPLLAAGPAAVTIANRTPDKAAEL
                     ALRFGDLGLCYGRSYDELAGERFDLLINATAAGLSGEVPPLPEGLLADAAWCYDMMYG
                     DEPTAFVRWARAHGAAHAADGLGMLVEQAAEAFLIWRGVRPDTAPVLAALRGEG"
     gene            complement(17537..17857)
                     /locus_tag="PK144_00245"
     CDS             complement(17537..17857)
                     /locus_tag="PK144_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64180.1"
                     /translation="MNRPNRMLALFAAAALLSGIGLAQAKTETIVTTITNPDGTVTRQ
                     VEQREVPNSVPDQSFEWADRDGNGCIDPREAKDMGILNFAKADTARRGCLGRAEYEHA
                     LISQ"
     gene            18134..18640
                     /locus_tag="PK144_00250"
     CDS             18134..18640
                     /locus_tag="PK144_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF64181.1"
                     /translation="MLADAMTAEIPRLRRYARALTGDPERADDLVQDCLERAWTRQHH
                     YDGSSELRPWLFTILHNLHANAARSYHRRPAFVDVDALAEAAPVAPNHDGELALRDLA
                     QAMDRLSDEQRAVLLLIGVEELTYPQAAAVLGVPVGTVMSRLSRARERLRQLLAGADD
                     GPRLRRVK"
     gene            18640..19455
                     /locus_tag="PK144_00255"
     CDS             18640..19455
                     /locus_tag="PK144_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNF64182.1"
                     /translation="MNGESGRRIAEAELHAWLDGRLEPERRAAVEDWLAANPGEAERL
                     ASYHTAEAGLRELYAPVLEEPLPARLLDVLAQPAPPQPPQAVQRPWLRAAMVAGCLLG
                     AGIAGAFGGWFGHARFGGDWPVAAGPDAAFVERAVYAHAVYVPEVRHPVEVAAAESQH
                     LFGWLSKRMGHTIRAPGLEALGFELLGGRLLPGEEGRPAAQFMYQNADGRRLTLYVNR
                     CDARGETSFRFHHEDSLNAFYWLDGELGYALVGDVSREELSAAAHQVYRQLAL"
     gene            19701..20558
                     /locus_tag="PK144_00260"
     CDS             19701..20558
                     /locus_tag="PK144_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF64183.1"
                     /translation="MLRFEGFAASRAAGGPQRLPALLLLGLLALARALPAAELEVGGP
                     AGSRRLDSAVLLATPATQDIDVPDDVAYGRPMRFRAVPLAPLLAAAGFTAADTVEAVA
                     LDGYVAQLPAAALLSDAADGAQAWLAIEPPDAPWPKLPRGEGTAGPFYLVWQEPQAGG
                     ITPEQWPFQLAVLRRVAAPAERWPALNPPAGAGSDAQRGLAVFVQHCLPCHRLNGAGE
                     ATIGPDLNRPMSPVEYFRPDLLRRYLREPAALRTWPGQGMPGFAESVLPAADLEALLA
                     YLAAMAAAR"
     gene            complement(20620..22002)
                     /locus_tag="PK144_00265"
     CDS             complement(20620..22002)
                     /locus_tag="PK144_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNF64184.1"
                     /translation="MPYAHAFGATRHRFADLKDLLAKATPLRSGDVLAGLAAETAEQR
                     VAAQYALAELPLATFLNEAVIPYEDDEVTRLILDSHDAAAFAPVAHLTVGDFRDWLLS
                     AQADAGTLAALAPGLTPEMVAAVSKLMRVQDLIVVARKCRVVTRFRNTIGLPGRLSTR
                     LQPNHPTDDAAGIAATLLDGLLYGSGDAVIGINPATDNLTQVTGLLHLLDAVIHEYRI
                     PTQACVLTHVTTTLAAIGRGVPVDLVFQSIAGTEAANAGFGVTLALLDEAHQAALALQ
                     RGPLGDHVMYFETGQGSALSAGAHHGVDQQTCEVRAYAVARRFRPLLVNTVVGFIGPE
                     YLYDGKQILRAGLEDHCCGKLLGLPMGCDVCYTNHAEADQDDMDALLTLLGTAGVTYV
                     MGIPGADDIMLNYQTTSFHDALALRATLGLRPAPEFEDWLYASGVFGADGRPAEALPA
                     AFAAALARIG"
     gene            complement(22382..23266)
                     /locus_tag="PK144_00270"
     CDS             complement(22382..23266)
                     /locus_tag="PK144_00270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF64185.1"
                     /translation="MTTTVRHAPALLALALALAVAGNATAQQPAPAPVAPPAAPAATP
                     APKPLPDPVAVVNGEPIAKSTFLTYQQQRARQMAAQGMPLDPDGSRAQIVDELVMQEL
                     LVQQAVKDKTAEDPEVAAQLDLLRRNMLATAVVRKYLKTAQPSEADIKAEYDKAVANM
                     KTKEYKARHILVDSEEKAKQMVAELKKGAKFEDLAKANSSDGSKDQGGDLGWFTPDMM
                     VEPFSQAVVGLDKGKYTETPVKTQFGWHVIQLEDVRDQTPPPLDELRPQLTQMLQGKL
                     VNDYLEKLKAGAKVEVKE"
     gene            complement(23333..23605)
                     /locus_tag="PK144_00275"
     CDS             complement(23333..23605)
                     /locus_tag="PK144_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNF64186.1"
                     /translation="MSAGRMQRIEACLRAAFDPVSDFAIFDDSAQHAGHAGAREGGHF
                     RVRIVSPAFAGLNAVKRHRAVYAALGALMQTDIHALGIDARTPDEP"
     gene            complement(23602..23904)
                     /locus_tag="PK144_00280"
     CDS             complement(23602..23904)
                     /locus_tag="PK144_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNF64187.1"
                     /translation="MFYALIGRDHPGTLEARLAARPAHLERLHALQAEGRLLLAGPLP
                     AIDSHDPGPAGFVGSLVVAEFESLEAARAWADADPYVAAGVYAGVEIHPFRKVLPA"
     gene            complement(23911..24468)
                     /locus_tag="PK144_00285"
     CDS             complement(23911..24468)
                     /locus_tag="PK144_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNF64188.1"
                     /translation="MKLFLDFLPVVAFFVAYKLADIYTATAAAIAASLAVVAYTWWRE
                     HRVEKMALVTAGLMVVLGGATLWLHDDIFVLWKPTAVNWLFAVVFLGSTFVGDRKPMV
                     RRALGAQIELPERQWRQLNLAWVTFFAVCGALNLVVAFHFDRDTWVNFKLFGLLGLTV
                     LFVFAQGWWLMRHLPEGRDSVARKE"
     gene            complement(24572..29191)
                     /locus_tag="PK144_00290"
     CDS             complement(24572..29191)
                     /locus_tag="PK144_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64189.1"
                     /translation="MQESEDDPKAGADDPAAHVERDAERDETEDGADSGPRPPLIVGI
                     AASAGGLEALGGLLRPLPATGRLAYVVVQHLSPSHPSLLVQLLQRECALRVVDVSDGQ
                     EIVADTVYVTPPRTNVSIHDGRLVLTEASDPGVPKPSADHFLHALAEDQGENAIAVIL
                     SGTGTGGAEGVRAVKAAGGFTFAQDDASAKYSGMPAAAIATGCVDLVLPPERIAEELM
                     RLAQLGVQGGLPVGSPHVPASVFERMLRLVQRHTGTDFLNYKPNTLRRRVARRMLATH
                     TATLEDYEMLLATQSGEIERLYQDILISVTAFFRDVPAFEALAGVLREMVAEKSPDDD
                     LRVWVPGCATGEEAYTIAILLAEALGTRLGERKVHIFATDLDTNALAIARRGVYHHSV
                     ASVVPPPLLERYFTTAGESYQVGKLLRDLVIFARHDLAQDPPFLRLDLISCRNLLIYF
                     KSPLQERVLDMFHYALRPGGWLLLGKSENPHTADLFAPSAGKVKLFRRRDAARARSLQ
                     MPRVPVRENDRATRPPARAGMTLEERVRLVALAAYAPAGIVVDEAMEVRYTLGDVSPY
                     IRLAEGKANLSITTLIRRELHVDLRGLILKCRSSNALTTSRRITMDGGPAVRLAVHPL
                     AGDFPEDPLFLVCFEHPRAEIETGRAGAESEQMQRTLELEQELAASREHLQTVIEELE
                     TSNEELQSLNEEMLSANEELQSTNEELETANEELQSTNEELITVNEELQSRSAQLMAA
                     NADLENLKNTLPCPVLVVDARLRLMFANPAADRLFGLDPDDSVGPLHALRANLDFSGL
                     REVVERVIDAGNEMTHQFAGDRDHLLRARPYRDAQGRIRGAVLVFWDNTDIRRAEERI
                     REQVRQMALQSRALAAADNGIVICDARAPDRPISYVNAGFTRITGYSAEEVMGRNCRF
                     LQGRETDRGIITEIARALADQRQISVLLRNYRKDGEPFWNELNISPMRDEDGVCTHFI
                     GVQNDVTERVQAAERARLALAVFENTSEGIMITDAHLHLVAVNPAFTAITGYRSEDAI
                     GQRPTLIASGRHGQDFYRQMWRELQAHGEWHGELWNRRRSGEVYPELLHIHVVQDDAG
                     HVTHYVGIFSDITELKAAERRMHEIAYFDSLTGLPNRSLFSDRLARALLRSRRDNTRC
                     AVLFLDVDRFKNVNDTWGHETGDKLLQEIGTRISGCIRGSDTLARYGGDEFVLLAEDV
                     GDGRRLSELAERIHDALTGPVSIDGQTIDTGVSIGIAICPQDGTEAEVLLASADAAMY
                     QAKHAGRHTHRFHDPQMNALWIERMALEHELRAALGGSGLELHFLPLVRLADRSIVGC
                     EALLRWHHGQRGLLAPAEFLVSVADVKLLGDIDRWVLRSAAAQHHAWAGTPAGALPMA
                     VNLSGALLRDGERLPGLLAEIAGSWLSFEFTETTLTEQGAPSPQLWAALHAAGLRLVL
                     DDFGTGRASLVQLRTLPLAVLKLHRSVYLDAGGERIDPAIVEAFVALARALGLELVAE
                     GVETEAEAVLLRDHGCPLAQGWLFGQPLSAGELPGRLGAAPPAAG"
     gene            29327..29962
                     /locus_tag="PK144_00295"
     CDS             29327..29962
                     /locus_tag="PK144_00295"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF64190.1"
                     /translation="MGERIEIHPVNPQPRLIRRAVEVLREGGVIAYPTDSCYALGCRI
                     GDKDAQTRIQRIRQIDDRRHNFTLICRDLSELSLYARVDNAQFRLLKALTPGPFTFIL
                     RATHEVPRRLQNEKRKTIGLRVPQHVIVAALLAELGEPIMSATLIPPGEDRAVSDPED
                     IETGLGRELDLIVDGGNCGIEPTTVLDLVEGLPPVLIRQGRGDASAFLAEA"
     gene            30040..30702
                     /locus_tag="PK144_00300"
     CDS             30040..30702
                     /locus_tag="PK144_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNF64191.1"
                     /translation="MPELNDIQRLAIMALPILFAITLHEVAHGWAALRLGDTTAWQQG
                     RLTLNPLAHIDPIGTIIVPLVLYFTAGFIFGWAKPVPVSMHRLPNPRRDMAFVALAGP
                     LANFVMATGWALVMALGAWLSHVLPELGQPLFFMGSAGIGVNVVLAVLNLVPLPPLDG
                     SRVLAAVLPARVGVQLYRFEQAGLILLVVLMATGVLGRVIGPIVEAVQQFYLWIVVSL
                     LG"
     gene            31005..32018
                     /locus_tag="PK144_00305"
     CDS             31005..32018
                     /locus_tag="PK144_00305"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNF64192.1"
                     /translation="MSKTRILTGITTTGTPHLGNFAGAIGPAIEATRRNPEAEFFFFL
                     ADYHALIKCQEPERVQRSRLEVAATWLALGLDPERVCFYRQSDIPEIPELTWLLTCVT
                     AKGLMNRAHAYKAAVQENLDSGDADPDKGVTMGLFSYPILMAADILMFNARKVPVGKD
                     QTQHLEMARDIAQRFNHIYGEHFVLPEAVVEEDAAVLAGLDGRKMSKSYNNTIPLFVS
                     AKELRKLVMRIVTNSQLPGEPKDPDGSTLFQIYQAFASADETAAIRVRYAEGIAWGEM
                     KQVLFEKLDSVLAGPREHYAALISDPAALERILLAGADKARAIASPFLAGLRAATGLR
                     KLG"
     gene            32043..32870
                     /locus_tag="PK144_00310"
     CDS             32043..32870
                     /locus_tag="PK144_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNF64193.1"
                     /translation="MTEGLSGTAGDATDAPGPAAAARIRGVALERLPEDLYIPPDALE
                     VLLDAFEGPLDLLLYLIRRQNLDLLDIPVAEITRQYLAYLELLHELRFELAAEYLAMA
                     AWLAEIKSRLLLPRPPRSDEDSEPEDPRAELVRRLQEYERYRRAAEDLDALPRLERDV
                     FAASAALPRHERVREPPHVELGELLGALREVLARAELHASHRVGREPLSIRARMSEIL
                     VRVDAVQFTRFTELFHAGEGRLGVVVTFIAVLELVREALLELVQSEAFAPIHVRRRA"
     assembly_gap    32872..32971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32972..33592
                     /locus_tag="PK144_00315"
     CDS             <32972..33592
                     /locus_tag="PK144_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64194.1"
                     /translation="SHPERIPSHIWCIPSHIEHIPSHIERIPSYIEHIPGCTECIPSH
                     IERIPSYIERIPSCTGRIPSYIECIPGCTGRIPGCTERIPSHIEHIPGCTGPIPGGDE
                     RIPAFVVPAPQACVPITMPIGRSARSSGSRRQRPQPCLPVAHQHEEKIIFRLAESGMT
                     VFHTAATRLAAFRPEGAMPAGRHLPFRRGSQGSQGVRNGQKCLCRP"
     gene            33564..33941
                     /locus_tag="PK144_00320"
     CDS             33564..33941
                     /locus_tag="PK144_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64195.1"
                     /translation="MARNAFADREAAFVRASQAWKRDDPEKLFAGLTQQAFADQVAEA
                     QRTSRQLEALLSQVDHLRNQRSAQFKKIAALNLRLKSAIVADPDHGSDSTLFEAFGGI
                     RVSERRSGLTRKRNDSTDDKTGT"
BASE COUNT         5092 a        12041 c        12329 g         4930 t
ORIGIN      
        1 ccgggggaag ggccggggag gagggggccg gcccgggctt caggcctggc cgagcgcgac
       61 cagcgcggtc gcaaagcgcg tgtcgagggc cggatcggcg gcggccgggg tgcagtacca
      121 gtcgtggatg acgctgtcgg catccggcgg cgcttcgccg gacagtgccg ccgacagccg
      181 ggcgatgcgg tgttcgagca gttcctgcgc gtcctcgggc ggcggttcga tgccggcgag
      241 gatctccagc tccaggcaca gccggtggcg gatcgacgcg ccctcggtca tccgcatcgc
      301 cagccggtgg gcctcgaccg gatcggccag cgcgcgctgc gcggcgtcga gccggctgcg
      361 gatgcgccgt tcgagcggtt cgggcagccg cgccagccgc tgccactcct cgcgcagcgc
      421 cgccggctcg tcgtcggcgc cgtcggcacc ggggtccccg gccgtggcgg gcgggccgtc
      481 gagcacgtgc cgttccagcc gcgcacagag cgtcgccttc tcggcgaggg catgcacggc
      541 cttgcgccgc tgcacgcgtt caagcccggt cagccgctcg tgccaggcat cgagggcggc
      601 cgcataacgc tgttcgatgg cgtgctgcgc cgcgcgcggc agctcgccga gcccggccca
      661 gtcctcggcg atgcgtgccg ccgcggcgcg ggcggcgccg gcgttgtcgg catcgagggg
      721 cgtggctgcc agcgtttcca gttcggcgca cagcgcggcg gcggcatcga gcgtcgcctg
      781 ccgctgcgcc tgaccggccg agcgcacctg ctcgcggcgc ccgaagatcg catcgcagac
      841 tgcccggaag cgcagccaca gcgcctgctc ggcatcgcgg cgcaactgca gcgtcggtga
      901 ccagtcggcc tgggcctggc gggcggcggc gatcgcggtg cgcaggtctt catcggccag
      961 gctctggagc tgcacgatca gcgcctcgcg ccgggcgcgc tcgcgctggc gctcggcctc
     1021 gatgcgtccg ccgacggcct cggcggcggc cttccagtcc tgctccagcg tccggtaggc
     1081 gttgcgcggc acggtgccgg cctcgcgcca gctgcgctgc agttcacgcc aggcctgatc
     1141 cagcgtccgc cagtccgggg ccgaccagtc ggtgccggca tcgagctgct gcaggcgttc
     1201 gatcagcgcg cggcgtgcgc cgatgtgctg ctcgcgctcg gccgcggcct gcgcgaaagc
     1261 ggcctgcgcc ggggcatagg cgcgttcgca ggcgctgttg aagcgttccc actgtccgcg
     1321 cgtcgccggc agctcgccgc cgtcgagctg cttccactgc gcgcgcagct gctgcacgcg
     1381 gcgcgcgagt tcggccggcg cccaggcgtc cggcaccagc ccttcggcgg cgaggcagag
     1441 ctgttcgcgc gcctgccggc cgccccagcg cgcccagtca cgcagttcgg cgagccgggc
     1501 ggcggcggcg tgcagccgcg catcgagggc gcgcaggccg cgggcctcgg tctggatctg
     1561 ctcgtacagc ggctgcgccg tatgcagttc gccggcttcg agggcggctt cgagggtttc
     1621 gagccgcgcg agcaattgcg catccggttc ggccggggca cggcgcggcg cggccggttt
     1681 cggtgcggag gactgcgttg gtgccggtgc cggctgtgcg gccagcagct gcgcgagccg
     1741 ggcgcgggcg cgttcgtcct gacggcggcg cagcgtggca tcggccggtg ccgggagcgc
     1801 ctgccattcg gcttcgagcc gggcccgggt cgcggcgtcg atcgacgcca gcgtttcgag
     1861 tgcgttcagc cagcgttcgc gggcttcggc atgggcggtg tcgcgcccgc gctcggccag
     1921 ccgctcgcgc agcgcctgca tgcgcacgac gatgcgcgga tcggccggag cctcggcttc
     1981 aagcccggcg agcgccgtgg cgcaggccgt ggcgtcgccg tgcgcggcga agccggcggt
     2041 gatggcatcg agggcgcgca ggcagcgggc gaccgcgacg ccgtgggcct gatgcgcggc
     2101 gagcagcggc gcgagccggg cctgcgcggc ctgacggcgg gccgggtcat cggcttcggc
     2161 cggcagctgc ccgaattcgc gttcgagcgc ggcggcgcgg accacgtcct cggcttccag
     2221 gcgctcgcgg gcggccagcg cgtcgagctc atcgagcagc gccgcggcgc gcaggcgctc
     2281 ggtctcgcgc cggcgcacgg tttccagccg ctcgctgacg agccggtaca ggcgcttgtc
     2341 gcgagcgcgg aagctgcgcg ccagcccggc gagcagttcg ggatcgtgga tgcgttcggt
     2401 ggccgcgagg cgcacctcgg caaccgcgtc ctgcacggcg atgacggcga gctgccgctg
     2461 atcgaggacg ccggcgagcg cggccagccg cagcgccgat tcggccgcct cggcggcgag
     2521 gtggacgagc agcgccggcg gcaggcgcgg atcggcaagc acggcgaggc gcaccgtcac
     2581 cgccggcccg ccggcgaggt gcccggcgag cagcgtgcgc agccggccgg tggcggcctc
     2641 gcgcacgacg gcgtcgtcct cggcgtgcag atgttcgagc agccgcagcg gatcgtgcag
     2701 ccggtcgagc gcggcgcggc gcacgcccgg atcgctgtca ccggccagtt cgagcagtgc
     2761 cggatcgccg tcggcgaggc tttgcagcgc gcgcaggcgc tcgcggggat cacgatggcg
     2821 ccacgacggc gcacggcgga acaggcgggt gaagatcatc ggtgactcgg caggtctcgg
     2881 gcgggggcga ttgaagcaca aagcggcggg cgcgtccgga gccggccggt tacgggacgt
     2941 ggcggggggc agcgggtcag cgcaggctca gccgtcgcgg cgcagcggct gcagcagcag
     3001 cgcgccgagg cgctgggctt gcggggcgcg gaagccgtcg aggccgatcg tcatcgtcgc
     3061 gtgtccgtcg cgcggtgccg agcgccaggt gccgaacagg tggtcccacc agacgacgct
     3121 gaagccgaag ttgctgtcgg tctcgtcgcg gtggatcgag tggtggatgc ggtgcacgtc
     3181 cggcgtgatc agcaggcggc gcagccacgc atcgaccggc gccggcagcg cgaggttggc
     3241 gtggttgaac agcgcacagg ccgacagcag cagctcgaag cccgcaaccg ccgcgaccgg
     3301 cgcaccgagc gcgagcacca gcacgatctt ccagagcatc gacagcccga tctccagcgg
     3361 gtgaaagcgc agcgccgtgc tggtgtcgaa gtcggtgtcg gtgtgatgca cgcggtgcag
     3421 gcgccagagc agcggcacgc ggtgcatcgc gacatgctgc gcccagatcg ccagatcgag
     3481 cagcagcagg ctcagcatcc cggcgagcgg cgccggcacg gcgagcgccc gcagcaggcc
     3541 ccagccgcgt gcctcggcga gcgccgcggc cccggtcgcg agcagcggca gcgtgaggcg
     3601 cagcagcacg gcatcgagcc cgaccagcgc caggtggcgt gcgacgcgcg gccagcgcgg
     3661 ctgcgtccag gcgcggcgcg gcgcgcgcga ttcgagcgcc agcagcgcga gcagcacgcc
     3721 gacgaacagc agcggacgca gccagccacc gtccatcgct cagctcgccg gcggcgcgtc
     3781 gaccggcgcc gcggcagggg tgggggatgg cccgctttcg gcgtcgtcgt cggtgtcggt
     3841 gtcgacggac ggcccgggcg cggcgcgcgg cggcaccggg gccagccagc cgaccagcag
     3901 cagcaggccg ccagtgccga ggaaggcgag gatgcgcgcc agcgtgtcgc ggccgtccag
     3961 atcgacgatg aacagcttca gcaccaccag cgccatcagc ccggcgccgg cgagccagat
     4021 gccgcgctgc ccgcggcgcg tgccggtgag catcagcacg accgccgccg tcgcccagac
     4081 cagcgccagc gcggtctgca cgaggcgcga atcccacagc gcagcggccg accacggcac
     4141 gccggcgagg tgatggcagg cgcgcagcac ggcgaggttc agcgtcgcga agccgagcgc
     4201 cgccagcacg gtgcgcgcgg ccggccgctg cgccggcggc acgaaccacg gtcggccgcc
     4261 ggcgcgcgcg agcaccagca gtgcggcgac agcagccagt tcgaccggat tcagcagcgg
     4321 cacgaacggc agcggcgccg gcagcgccgg gccgtgcagg ccggcggccg ggaaccacgc
     4381 gagcagcagc agcgccaccg gcgcgccggc gagccggcga ccggccgtgc cgtacgcggc
     4441 gagcggccac gccgggcgct cgccatcgag cagcagcgcc agcgtcgccg cgatcagcac
     4501 gccgttggcg gcgcgcgtcc acacgaaagc gagatgcacg acatcgccga tcagccacgc
     4561 cacctcctgc agcgccagcg ccagcagcag ccagacggcg gccgcgtagc gccagcgctg
     4621 gccgcgatca ccggcatcgg ccgcggagga ttcgacgcgc agcagtccga actgcacggc
     4681 gaacgccgcc agccagccgg cagcgccgag gccggcgagc gggtggtcgc ggtgcaggaa
     4741 gtcgtgcatg gccagcggcg cgagcagcag cggcagcccg tacagcggca cgcgcagcca
     4801 gccgagcgcc agccgccgcc aggcgaggaa ggcgagcgcg ctgctcgcgg caaggaacag
     4861 cagccagacg ccgctcgcgg catcgaccgc cagcgtcgac atcgccgtct cgcgcaggca
     4921 ccacagcagc caccagaacg ccccccagcc ggcgagcacg gtttcgaggc gcagcggctg
     4981 cggtgccggg cgcaggcgtc cgagcgcggc ggcggcgacc agcgccgcga gcgcgagcag
     5041 cggcgtgtcg agcgcgtcgg cgcgcaggaa cggccgcgac accggcatat cgggcaggtg
     5101 cgcgtccagc cacagcgccc cggcggcgaa cagcagcagc gcgccgaagc cggtcagccc
     5161 gcgccggtcc tgccgggcgc cgacccagac cagcccggcg ccttcgagcg cccacagcgg
     5221 gctcgacgtg ccggcctcga agtacagcgg cacggcgagc gtcgccgaca ccagcgcgat
     5281 cgctgcctga cattcgccga gcagcgcgag cgccggccgc tgccggcaga agcgccagag
     5341 cgccgcatac caggccgcgc agccgagtgc gctcagcgcc agtccgtgtt cgacgccttc
     5401 gagcagcgcc gcctgcagcg ccagcgtcag caccggcacg ccgaacacca gcccggcatc
     5461 gagcgggcgc ttcgggtccg gcggcagccg gtgcgcggcc agcaccggca gcagcgagaa
     5521 cagcccgaag aacgcgagca ggaagggctc ggtgctcgcc agcagctccg gccggtagcg
     5581 cagcccgccc cacagcgtgc cgatcacgta ggtgcagacg aagccgagca cggccagccg
     5641 ccgccacggc tcgcgcagca gcagcgccag gatgccgaga tcgagcacga ggtagtagcc
     5701 gaacagcgcg acgtgatcgt tgctgccggt cgagaccagc accggcgcga gaaagccgcc
     5761 gagcaccgcg aacagcccga gcacctcggc ggcctgcgcg accgcgagcg cgacgccggc
     5821 tgccgtgcac gcgaacagca gcgcgaaacc ggcgagcggc gggatcagcc cgtagaactg
     5881 catcgcgccg tacagcacca gatagaccag gccgaggccg ccgccctgca ggctcagcgc
     5941 gtaaccgcgc cggcgctcgc gcagccgcca gccgagcgcg acgccggcga tgccggtcgc
     6001 cgccagggct gcgaggcgca gctcgatcga cagctgcgaa tgctcggcga cgtagcgcac
     6061 cagaaaggct gcgccgaaga acagcacgac gatgccggta cgcaccagcg cgttgccacc
     6121 ggcgagccag tcctgcagcc gcgagcgcag cgaagccccg gccgcaccgg actgcggggt
     6181 gtcccagccg ggcgggatcg cggcctgcgg aaagccgggc tgccagccat cggggggcgg
     6241 cgccgctgcc tgcgcgacag ccgcgggctc gacggctgcg cgggtccaga cggtttcgcg
     6301 ggccgggacg gcggcggggc gtaccgcttc agcgggcgcc ggggccggct ggccggtgcc
     6361 gggcgcatcg agccgggctt cgagccgctg cacgcgccgg cgcagggcga gcagcaggcc
     6421 gagcgtcagc ccgagcgtgg cgccgagcac ggcaccggcg gcgtcatcgc gggtggcctc
     6481 ggcgccggtg atggccccga ggatgatcag gaacagcgac agcagcagtg gcatcggcag
     6541 cggcgatccg gcggtgcggg gcaggcatgc acccgtcggc atcactatat gcaccggccg
     6601 ggcgggctgg tgctgcgccg cgtcacgccg ccggccggcg tgcgtggcaa actccgcgcc
     6661 cttgtgcgcc gttgccgccc cggagtgttg ccgacatgcc gcagaccttc ccgtcccgcc
     6721 accccgccgc ccgtccgcgc cgcctgcgcc gcgacgagtt ctcgcgccgg ctggtgcgcg
     6781 agaccgtgct gacgaccgat gacctgatcc agccgctgtt cgtgatcgag ggtcacggcc
     6841 ggcgcgaggc ggtggcgtcg atgcccggcg tcgagcgcgt gaccatcgac gaactgctgc
     6901 gcgaggccga acagctggtc gcgctcggcg tgccggcggt ggcgctgttc ccggtcaccg
     6961 cgcccgaggc caagagcctc gatgccgcga gcgcgtacga cccggcgggg ctcgcgcagc
     7021 gtgcggtgcg ggcgctgaag gagcgcttcc cggagcttgg cgtcatcacc gacatcgcgc
     7081 tcgatccgta caccagccac ggccaggacg gtctggtcga tgccgatggc tacgtgatga
     7141 acgacgagac catcgaggtg ctggtgaagc aggcgctgtc gcacgccgag gccggtgccg
     7201 atgtcgtggc accgagcgac atgatggacg ggcgcatcgg cgcaatacgc aatgcgctcg
     7261 aagccgccgg ccacatccat acgcgcatcc tcgcgtattc ggccaagtac gcatcgagtt
     7321 tctacgggcc gtttcgcgat gcggtcggtt cggccggcgc gctcggcaaa ggcaacaaat
     7381 acacctacca gatggacccg gcgaacagcg acgaggcgct gcgcgaagtc gcgctcgatc
     7441 tcgacgaggg cgccgacatg gtcatggtca agccgggcat gccgtatctc gatatcgtgc
     7501 ggcgcgtgaa gagcgaattc ggcgtgccga ccttcgtgta tcaggtcagc ggcgaatacg
     7561 cgatgcagat ggcggcgatc cagaacggct ggctcgccga agcggtgatc gccgaatccc
     7621 tgctttgcat caagcgcgcc ggggccgatg ccgtattgac gtatttcgcg aaacgcgtcg
     7681 ccgccgatct gcgggaacgc ggctgatgcg ccggccgggg ctgtggatcg gcgcggcggc
     7741 actcgtgctc ggcgtgctga ccgtgccgct gttcgcaccg ggcagcaagc cgtcggtgcc
     7801 cggcaccgac ctgccctggc aggtcgaaac cgacgcgcac ggcgtgccgg cggtgttcgg
     7861 catccggctc ggcgagacgc gcctgcagga cgtgctgccg cggctcgggc gcagcgtcga
     7921 actcggcctg ttccggcagg ccgacggccg cagcgtgctc gaagcctatt accgcgagat
     7981 cgagctcggc ggcctgacga ccaagctgat cctgcgtctc ggcgccggac aggaggcgct
     8041 cgatgcgatg atcgcgcgtg ccggcaaggg cgatccgacg ccgtcgggct cgatgcagta
     8101 catcccggcg agcaccgacg accacgccat catcgcgctg ccgatcatcg gtctcaccta
     8161 ttcgccgctg gtgcggctcg acgaggacgt gatccggagc cgtttcggcg agccggccga
     8221 aaagctggcc gagaaggacg gcgtgcactg gctgtatccg gcgcgttcgc tcgatctgtt
     8281 ccggcaggac aacggcaagt cgatcctgac ctacctgctg ccggccgatt acgcgcaatg
     8341 gcgcgcgcag taccggccgc cgtccgggca atagctctgc tgccggcaca aaaggccggc
     8401 agcatccgtc cggcctgcct cgacctgcga ccggcagcca tccggctgcc ggtttcgttt
     8461 gccggtgcga tcacgccgat cccggctgaa cgtccggtct tttcagccct tccctcctcg
     8521 cgggggatgg gttggggaga gggggatcaa ccgcgcattc acgtgtgatc ggcatcattc
     8581 ctctgtcccg atttctccgt gcgagcggga tggtttggaa aggacgacgt gctgatctgt
     8641 ggccggcatt ccgggcgtcg catgccggaa gtccggcatc gccggaaatg cagaggattg
     8701 cattacggtg cggatgcgga attttccggt cctggtgaaa ggcaggggat tgcaggaatc
     8761 cgcgccggat cgtaatgcgc tcgaatgcca tccggcttgc ggccatggat ggattccgtc
     8821 cgtgtgcgcc gtccgtgacg gaggaatccg gtgccgggag cgggccgggg cgacagggcg
     8881 gtgaccggtg gccggcgcac tgctgccggc cgtcacggct ttggccggac ccgatggcgg
     8941 ctgctttcgg aggatggcgg cctgttttgt aaaacactgt tttttatggg aatgtctgga
     9001 tgtgccggcc gcttgtccgg gttgcgggtg gtctgccatg ggtgccgtgc ggggagcaag
     9061 ggtctgcggg ccgggcacga cctgccatgc attcctggaa gcccgtgcgc cacattccgt
     9121 taccgagttg acgattggtg attggctgaa gatcgtttcg ttacgcatgc tgacagggtc
     9181 ggaaagccga tggatgccag cgggaagcgc gcatcggctg cagcggctgt aaaacgctgt
     9241 gacactggat tccgccaggg agatcaggca gattatcctt tagttaatgt ttcgggtgac
     9301 ggactttcga cgattcgacg cccggatttg atgctcagta tcgggtgtca agatttcgac
     9361 gaaccggaac ggcacctgac ccataacgag gatcgcggcg gcaaacccgc ccgaaacaat
     9421 gaaatgaacg ccacgcactg cccccgcatc cgccaggacg gcttcacgct ggtcgaactg
     9481 atggtcgtgc tgtccgtggc ggcgatcctg accacgatcg gtgttccttc cttcctgtcg
     9541 acgatccgcg acaacggcgt cagcacccgg accaacgagc tgatcaccga tctggcgctg
     9601 gcacgcaccg aggcgatccg ccgcggccag accgtcaccg tctgcccgag caccgacggc
     9661 acgagctgcg ccggcagcgg cggctgggag cagggctgga tcagcttccg ggacaacgac
     9721 gcttccggcg tgctcgacag cagcgactgc tcgacgccgg ccgactgcgt gctgcgcagg
     9781 agcccggcgc tcgccagcgg ctacacgctg cgcgccaaca acgtcacgct cgctgcgcac
     9841 ctgagcttcg acggcatcgg catggcgacc gcgagcttcg gccagctgcg gctctgcgac
     9901 gagcgcggca tcagccacgg ccgcatcatc gacgtggctg ccgccggccg ggcggtggcg
     9961 aagcagcctt ccggcagcga cagctgcgag tgatgagccg gtgccggcga tgacggcagg
    10021 cgcgtcgccg ggccggcagg tccggcgccc gcatcgggga cgggggacgc gggcgtggac
    10081 ggcaggcggc aggtgcaggg attcacgctg atcgaggtgc tggtcgcgct gctgatcgcg
    10141 gcgatcggcc tgctcggcat ggcggcgctg catctttcgg ggctgcgcag cgtcgagacc
    10201 gcctaccagc gcagcctggc gacgctcgcc gccaacgacc tgctggagcg catgcgcgcc
    10261 aacgccgatg cggtgcgcgc cggcaactac gcaagcgctt acggcgcggc cgccaccgcc
    10321 tcgaccgact gcgaggcgac gagctgctcc ggcgcacaga tggcggcctt cgacctggcg
    10381 cgctggaagg cttcgctcgg cgcgctgccg gccggcgacg gcaaggtcga gatgagcggc
    10441 acgcgcgtca ccgtcacggt gcgctgggac cattcgcgat cgggggacgg gacgcaatgg
    10501 gcgagcttcc tgatgaccac cgacttctga acacgcgcgc cccacgctgc gccgccggcg
    10561 gtttcacgct ggtcgagatg atgctcgcgc tggcactcgg gctgctgatc gtcgccgggg
    10621 cgctcgccgt ctacgccgcc aaccgcgccg tctaccagca tcaggaagcc tgggcgcggc
    10681 tgcaggagaa cgcgcggctg gccttcgagc tgatggcccg cgacggccgc caggccggct
    10741 tcatggcctg cctgccggtg gccgccccgc tcaccaacgt gctgaacagc gcgacgacct
    10801 acgaatggct gttcgaacag ccggtcgacg gcttcgaggc gagcggcgtg tcgtggtcac
    10861 cgacgctgcc ttcaggcttt ccggcgccgt accgcggcac cgacgtgctg acgctgcgcg
    10921 gtgcgcagtc ctcgccgcag gcgcgcgtca gcgcccaggc cagcacctcc gccgacgtga
    10981 cgctcgacaa cggcagcggt ttcgcgagcg gcgacttcgt gctgctggcg gcggccgact
    11041 gctcggaagg cgcgatcttc caggtgagcg gcgtcacggc cggggccgcg ccggtcgcct
    11101 cgctcgcgca cgccgccggc gccggttcgc ccggcaacgc cagtgccgat ctcggcgcgc
    11161 gctacgtcgg cggactggcg ctgaagctga ccacgtattc gtactacgtc gccgccggca
    11221 gcgacggccg cccggcgctg taccgccgcc agggcagcgc gacgccggtc gagctgatcc
    11281 cgggcgtcga tgacctgcag gtgctctacg gcctcgccga cggcagcaac ggcgactatg
    11341 ccagccgcta cgcgacggct gcagtgatca gcgccgatgc cgataactgg cagaagatcg
    11401 tcggcctgcg cgtgacgatg acgctggaaa gcccggacga cggcatcacc gacagcgcgc
    11461 aggccgtgac catcaacggt gtcagcggca gcgaccgccg cctgcgccgg accttcacgg
    11521 ccacgatcgc gatccgcaac cgcctgccat gagcgccacc ccgttttgcc gccctccggc
    11581 ttcgctgcgc gcgccgcgcc gcgagcgcgg tgccgtgctg atcatgacgc tgatcctgct
    11641 ggtggtcgcg acgctgatcg cgctgtcggc gcagcgcaac accgcgctcg aagagcgcat
    11701 ggccggcaac ctgctcgacc gcaacctgtc cttccaggcc gccgaggcgg cgctgcgcgg
    11761 cggcgagtcg gcgctcgcca atgccgtggc cgcgggcagc gtgccggtgc tctgcagcgc
    11821 cgatgccggc aactgcgcgg ccggctggta cacggcggcg agcggcgccc agacgccggt
    11881 ctggaagcgc gtcggctggg gcgacggcaa ctcggtgccg atcgccgtgg cgccgccctc
    11941 gggcagcacg agtccgctcg ccgcgaaccc gcgctacatc gtcgagcagc tcgatgcctc
    12001 gacctcgacc tggcgggtca cggcgcgggc gaccggtggc tcgggcgccg gcgtcgtcgt
    12061 gctgcagtcg atctacatca ccgaataaac caaccagaaa cgaccggccg ggccggcccg
    12121 gcgggaaggt atccgatgtc cgtccgctgc atccgcccgc cgaaaccgtc gtccctgtgc
    12181 gtcctgctgc tgctgttcgc cggcctgctg ctgccggggc tggctgcggc gctgaatctc
    12241 gcgcaggccc cgatctatca ggcaccgccg cgcaagccgc tggtgctgct gtcgatgtcg
    12301 aaggaccaca cgctgttcta caaggcctac gacgactact ccgacctcga cagcgacggg
    12361 gtggccgaca ccacgtacag aaacgcgatc acctattacg gctatttcga cagcctgaag
    12421 tgctactcct acgacacgac gaaccagcgc ttcgagccgg ccgcagcgag cagcgaccac
    12481 tactgcagcg gcacgacctg gagcggcaac ttcctgaact gggtgtcgat ggcgcgcatc
    12541 gacgtggtgc gcaaggtgct ctacggcggc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnacctacgt gaccacggcg acgacgacga gcaccgtgac gccgggcatc
    12721 gtcgtgctcg aacgcagcta cctgccgaac gacgcgcaca gctggagcaa ggtctacgcg
    12781 ccttcgggga ccagcgacat cggccggctg acgccgctcg gcgcgcgcgc gtcgatctcg
    12841 atctgcaaca ccacgtatgc cgcgagcggc agctactcgc agcgcgtcga caccgccgcc
    12901 tatccgccgg tcatgcgctt cgcggccggc gagtttccgg actgggcggc gcaggaggtg
    12961 cgccagtgcc agaccggcgt gagcggcagc cccggcagct cggcgctgat cggtgactac
    13021 gcggcgcgcg tgcgcgtctg cgtgcccggc agcggcaccg gcggcctcgg cggcgaaaaa
    13081 tgcaagcagt atccggacgg cagctaccgg ccggtcggcg tgctgcagga ctacggcgac
    13141 gacgaccaga tgtacttcgg cctgatgctc ggcacctatg gcggcaacaa gtcgggcggg
    13201 gcgctcagga agaacatcgc ctcgtggcgc ggtgacgaga tcaacctgca gaccggccag
    13261 ttcctggcaa ccccgtcgac cggcagcatc cgcgccgcgc tcgatgcgct gcgcctgttc
    13321 ggctacaagc acggcggctc gtataccgac agctacaaca gcggcaacgg cggtgatgcc
    13381 tgcggcctgg cctcgaacac cgacccgctg aactcgttca ccgagggcaa ctgttcgacc
    13441 tggggcaacc cgatcgccga gctgacgctg gaggcgatgc gctacttcgc cggcctgcgc
    13501 tattcgacca gctcgaccgc caacggcctg agtccgacca cggccttcaa caaggacgac
    13561 agcgcgcgct tcgccgacct gaagctggtc agctggcaga acccggcgag cagcgaccag
    13621 tacaagtgct cgccggcgag cctgatcgcg atcaactccg gcgtcagttc ctacgattcc
    13681 gaccagctcg gcggcttttc ggacctcggc atcagcggca cgctgtccgg ctacaccgac
    13741 gcgatcgggg cggccgaagg catcagcagc agcggcaccc cgctgttcgt cggcgagttc
    13801 ggcagcagca ccgaccgccg ctgcacggcc aagaccgtca cggcgctctc cggcgtcaag
    13861 gggccgtgcc cggaggcacc gcgcctgtcc ggcagctacc agatcgccgg gcttgccaac
    13921 gccgcgcata ccgccggcgt cggcgggcgc aaggtcgatt tcttcggcgt gtcgatggcc
    13981 ccgccggtgc cgaagatcac gatcccggtg ccggcctcga cgcgcaccgt gacgatcctg
    14041 ccggcgtgca aggacagcaa caacgactcg gcctgcacga tcgtcgactt ccgcatcctc
    14101 agccagacgg ccaccgccgg ccagtacgcc gggcgcgtgt cggtggtctg ggaggacggc
    14161 gagcagggca acgactacga cgaggacatg aagggcacga tcgactacgt ggtcaccagc
    14221 aacaccgtga cggtcaccac ctggatcgac tacgccaacg ccggccacac gctcggcttc
    14281 ggctacatca tcagcggcac ctcgcaggac ggctttcacg cgcactcggg ccacaacacc
    14341 ttccgctaca ccgatgcgac cggcgtgcgc gactgcagcg ccggctgctc ggtcagcagt
    14401 ccggcggtgc agaccgcgca gacctacacc atctccggca ccgctgccgg cacgctgcgt
    14461 gcgccgctgt actacgcggc gaagtggggc ggtttccgcg accgcaacag caacggccgg
    14521 cccgacgacg gcgagtggga ggaccacttc ttcgacgtga agaaccccgg cgagctgtcg
    14581 ccggcgctga gccggctgtt cgcgtcggtc gccgccggcg acccgtcctc ggcggccctg
    14641 agcgccaacg tgcgcacgct gcagacctcg gcgctgctgt tccaggcttc ctataacccc
    14701 gccgactgga gcggcgaggt caaggcctac ggcatcgacg ccgccaccgg cgcgctgtcc
    14761 gggctgatct ggaaggcgag cgatcacctg ccggcgaccg cgagccgcaa catcgtcacc
    14821 tggcgcccgg gcagcggcgg catcgccttc acgtggacga atctcgacag cacgcagaag
    14881 gcggcgctgg gcaacgacag cacgctgctc gactggctgc gcggcgacca gagccgcgag
    14941 ggcacggcca gcaaccagct gcgcgtgcgc acgacccgcc tcggcgatgt cgtcaacagc
    15001 gatccggtgt tcgccggcgt cagcgacgcc ggatacggca ggctgccggg cagcgagggc
    15061 gcgagctacg cagcctgggt cacggccaag gaaagcctcg cgcagatggt ctacgtcggc
    15121 gccaacgacg gcatgctgca cggcttcgat gcgaccaccg gcagcgagcg cctcgcctac
    15181 gtgccgaacg ccgtgttcgg ccgcctcgcc ggcctcacgg cgcgcagcta cagccacgtc
    15241 tacagcgtcg acggcgcgcc ggcggtcggt gatgtccatg acggcagccg ctggcgcacg
    15301 ctgctggccg gcacgctcgg cgcgggcggc aaggcggtgt tcgggctcga tgtcagcgat
    15361 ccggccacgt tcggccccgg caacgtgctg tgggagttca ccgacgccga tctcggcgag
    15421 ggcgtgcgtc aggcgctgat cgtgcgcacg cacgacagcg cgcacccgtg ggtcgtgctg
    15481 ttcggcaacg gctacaacgg cacgagcggc aacgccgtgc tgtacgcgct cgatgcgctg
    15541 agcggcgtgc agctcgcgaa gatcgtgctg ccggtcagcg gcgccagcgg tctcgcgacc
    15601 ccggtcgcct gggatgccga cggcgatctg gtcaccgatg ccgtctacgc cggcgacctg
    15661 ctcggcaacc tgtggaagtt cgacctcagt ggcagcgccg gcggctggcg ggtggcgctc
    15721 ggcggccagc cgctgttcac ggcgcgcggc ccgaccgggc tcgcgcagcc gatcaccgtg
    15781 cagccgaccg tcggcaaggg caaggacggc gtcgtgcgcg tgttcttcgg caccggcaag
    15841 tatctcgaag ccagcgacgg ccagatcgag acctcgccgg cggtgcagag cttctacggc
    15901 ctcgtcgatg ccggcagcag catcacctac accagccgca gcagcgtgct gaccgcgcag
    15961 accctcatcg ccgagttccc ggccggcacc aacggcccgt atccgctgcg ggtcagctcc
    16021 gacaacacgc cggccgacgg ggcgaagggc tggtatctgg acctggtgcc gccgaacggt
    16081 gtcgcggccg gcgagcgtgt cgtcgagcgc gccgtgatcc gttacgaccg catcgtgttc
    16141 ccgaccatgc tgccgcttgc gagcgccggc tgctcgggtg gcggcgacgg ctggctgatg
    16201 gaggtgctgt ccgccagcgg cggccggccg aagaccgcct cgttcgacct gaacaacgac
    16261 gggcgcttcg atggcagcga cggcgtgacg atcaacggcg agctcgtcgt cgtcaccggc
    16321 atcaatccgc aggtcggcgt catcagcggc gcgccggcgg tggtcgtcgg cgccaagtcc
    16381 gaaaccaaat gggtgaccgg ctccgccggt ggcacgccgt tcagcgtcgg cgagcgcatc
    16441 gatgcgcgca gcaccgggcg gcagtcgtgg atccagctgc agtgagggcg tgacggcggg
    16501 acggcgggaa ggacagggcg gcagcgcggg cgacggcgtt ttttctcctc ccgccgcccg
    16561 ccgtccgctc ctacactgcc ggcccccgac ctcacccgga agccgccgtc atgaccgacc
    16621 gctacgccgt gatcggcaac ccgatcgccc acagcaagtc gccgcgcatc cacacgctgt
    16681 tcgccgcccg gaccggacag gatctcgtct acgaggcgat cctcggtccg cagggcggtt
    16741 tcgccgactg cgccacggcg tttcgcgcag ccggcgggcg cggcctgaac gtcaccgtgc
    16801 cgttcaagca ggacgcctgg gtctgggccg atcgcctgag cacgcgcgcc gagcgcgccg
    16861 gcgcggtcaa cacgctgaag ctcgacgagg acggcgtgct cggggacaac accgacggcg
    16921 ccgggctggt gcgcgacctc gaaaccaacc tcggctgcac gctggccggg cggcgcgtgc
    16981 tgctgctcgg ggccggcggc gcggcgcgcg gcgtgctggc accgctgctg gcggcaggac
    17041 cggcggcggt gacgattgcc aaccgcacgc cggacaaggc cgcggaactc gcgctgcgct
    17101 tcggcgacct cggcctctgc tacggccgca gctacgacga gctggccggc gaacgcttcg
    17161 acctgctcat caacgccacc gccgccgggc tttcgggcga ggtgccgccg ctgcccgaag
    17221 ggctgctcgc cgacgctgcc tggtgctacg acatgatgta cggcgacgaa ccgaccgcct
    17281 tcgtgcgctg ggcgcgcgcg cacggcgccg cgcatgccgc tgacggtctc ggcatgctgg
    17341 tcgagcaggc ggccgaggcc ttcctgatct ggcgcggcgt gcgtccggat acggcaccgg
    17401 tgctggcggc cctgcgcggg gaaggctgaa gcggtgcggc agggcagggg ccggaacgac
    17461 gacgggcggt ccgcagaccg cccgccggcg tggctcgtga agcaggtcgt gaaaccggct
    17521 tcgtgcgctg actgccttac tgcgagatca gcgcgtgctc gtactcggcg cgccccaggc
    17581 agccgcggcg ggcggtgtcg gccttcgcga agttcaggat gcccatgtcc ttggcttccc
    17641 tcgggtcgat gcagccgttg ccgtcccggt cggcccactc gaagctctgg tcgggaaccg
    17701 agttcggcac ttcgcgctgc tcgacctggc gggtgacggt gccgtcggga ttcgtgatgg
    17761 tcgtgacgat ggtctcggtc ttggcctgcg cgaggccgat gccggaaagc agcgcggccg
    17821 cggcgaacag ggcgagcatg cggttcgggc gattcatggt gaaggtctcc ttctggatct
    17881 ggcctgatct ggcctggtct ggatcggcac ggaagcggcc ggtgggcgcc tgcggcagca
    17941 ggccatccgt ccccgctgcg ggcaggggcg gaccgtaacg caaggtggga tggcggatcg
    18001 ccggcgaagt tcgcggatcc cccgggaagg gctgcaacct ttggggtgta gacgtggtgt
    18061 acgtgcaggg tagaacacga actccggagg tccagacctt gaaccgtcgc aggaagcccc
    18121 gtccgtgagc cgcatgctcg ccgacgccat gaccgccgag attccgcgcc tgcgccgcta
    18181 cgcgcgcgcg ctgaccggcg acccggagcg tgccgacgat ctggtgcagg actgcctgga
    18241 gcgcgcctgg acccgtcagc accactacga cggcagcagt gagctgcggc cgtggctgtt
    18301 cacgatcctg cacaacctgc acgccaacgc cgcgcgcagc tatcaccgcc ggccggcctt
    18361 cgtcgatgtc gacgcgctgg ccgaggccgc accggtggcc ccgaaccacg acggcgaact
    18421 ggcactgcgc gatctggcac aggccatgga ccggctctcc gacgagcagc gcgcggtgct
    18481 gctgctgatc ggcgtcgagg agctgaccta tccgcaggcc gccgccgtgc tcggcgtgcc
    18541 ggtcggcacc gtgatgtcgc gcctgtcgcg ggcgcgcgag cgcctgcggc aactgctggc
    18601 cggggccgat gacggcccgc ggctgaggag ggtcaagtga tgaacggcga gtccggcagg
    18661 cgcatcgccg aggccgagct gcacgcatgg ctcgacggtc gcctcgaacc cgaacggcgc
    18721 gcggcggtcg aggactggct ggccgccaac cccggcgagg ccgagcgcct cgcgagctat
    18781 cacacggccg aggccggcct gcgggaactg tacgcaccgg tgctcgaaga accgctgccg
    18841 gcgcgactgc tcgacgtgct cgcgcagccg gcacccccgc agcctccgca agccgtgcag
    18901 cggccgtggc tgcgggcggc gatggtcgcc ggctgcctgc tgggcgctgg catcgccggc
    18961 gcattcggcg gctggttcgg acacgcgcgc ttcggcgggg actggccggt ggcggccggc
    19021 cccgacgccg ccttcgtcga gcgcgcggtc tacgcgcacg ccgtctacgt gcccgaggtc
    19081 cgccatccgg tcgaggtcgc cgccgccgag agccagcacc tgttcggctg gctgtcgaag
    19141 cgcatgggcc acacgatccg cgcgccgggt ctggaggcgc tcggcttcga gctgctcggc
    19201 ggccggctgc tgccgggcga ggagggccgt ccggccgcac agttcatgta ccagaatgcc
    19261 gacggccggc ggctgacgct gtacgtgaac cgctgcgatg cccgcggcga gacctccttc
    19321 cgcttccacc acgaggacag cctgaatgcg ttctactggc tcgacggcga actcggctac
    19381 gcgctggtcg gcgacgtgag ccgcgaggaa ctctcggccg cggcgcatca ggtgtaccgg
    19441 cagctggcgc tgtgagcggc ggccggcggc ccggggcctc ttgccggacc ctgccgtgcc
    19501 tgccgctcag ccgggaaggc agggtctttc ttccgctctc cccgatcccg cttcccccgc
    19561 cgggcggcgg gttttttgtc gatacttgcc gtggtgtgtc gtcgtcctga tccggcagtg
    19621 gttcccggac tcgtccggga gccgctgcgt ccgggagtgg tgcagatacc gatgccaggg
    19681 gaacaggggg agggatggtc atgctgcgct tcgaagggtt cgcggcgagc cgtgccgcgg
    19741 gaggcccgca gcggctgccg gcgctgctgc tgctcggcct gctggcgctg gcgcgagcgc
    19801 tgccggcggc cgaactcgaa gtcggcggcc cggccggcag ccggcggctc gacagcgccg
    19861 tgctgctcgc cacgccggcg acgcaggaca tcgacgtgcc cgacgatgtc gcctacggcc
    19921 ggccgatgcg ctttcgcgcc gtgccgctcg cgccgctgct ggcggcggcc ggcttcacgg
    19981 ccgcggacac ggtcgaggcg gtcgcgctcg acggctacgt cgcgcagctg ccggccgcgg
    20041 ccctgctcag cgacgccgcc gacggcgcgc aggcctggct cgcgatcgag ccgccggatg
    20101 caccgtggcc gaagctgccg aggggcgagg gcacggccgg gccgttctat ctggtctggc
    20161 aggagccgca ggccggcggc atcacgcccg agcagtggcc gttccagctc gccgtgctgc
    20221 gccgggtcgc ggccccggcc gaacgctggc cggcgctgaa tccgcccgcc ggtgcgggca
    20281 gtgacgcgca gcgcggactc gcggtgttcg tgcagcactg cctgccctgc caccgcctga
    20341 acggtgccgg cgaggcgacg atcggcccgg acctgaaccg gccgatgagt ccggtcgaat
    20401 acttccgccc cgatctgctg cgccgttacc tgcgcgagcc ggcggcgctg cgcacctggc
    20461 cggggcaggg catgccgggc ttcgccgaga gcgtgctgcc ggcggccgat ctcgaagcgc
    20521 tgctcgcgta tctcgcggcg atggcggcgg cacgctgagc gccgccggcg atgcggtcgc
    20581 ggccggacgc catgcggctg gccggccgtc gccgggcgtt cagccgatcc gcgccagcgc
    20641 cgcggcgaag gccgccggca gcgcctcggc cgggcggccg tcggcgccga acacgccgct
    20701 tgcgtacagc cagtcctcga actccggcgc cgggcgcagg ccgagcgtcg cgcgcagtgc
    20761 cagcgcgtcg tgaaacgagg tggtctggta gttcagcatg atgtcgtcgg cgccggggat
    20821 gcccatcacg taggtgacgc cggcggtgcc gagcagcgtc agcagcgcgt ccatgtcgtc
    20881 ctggtcggct tcggcgtggt tcgtgtagca gacatcgcag cccatcggca ggccgagcag
    20941 cttgccgcag cagtgatcct ccagcccggc gcggaggatc tgcttgccgt cgtagaggta
    21001 ctccggaccg atgaagccga cgacggtgtt gaccagcagc ggccggaagc ggcgggcgac
    21061 cgcgtaggcg cgcacctcgc aggtctgctg gtcgacgccg tggtgcgcgc cggccgacag
    21121 cgccgagccc tggccggtct cgaagtacat gacgtggtcg ccgagcgggc cgcgctgcag
    21181 cgccagcgcc gcctgatgcg cctcgtcgag cagcgcgagc gtcacgccga agccggcgtt
    21241 ggcggcctcg gtgccggcga tcgactggaa gaccagatcg accggcacgc cgcgcccgat
    21301 cgccgcgagc gtcgtcgtca cgtgcgtcag cacgcaggcc tgggtcggga tgcggtattc
    21361 gtggatcacc gcatcgagca gatgcagcag cccggtgacc tgcgtcaggt tgtcggtggc
    21421 cgggttgatg ccgatcaccg cgtcgccgct gccgtagagc aggccgtcga gcagggtggc
    21481 ggcgatgccg gcggcatcgt cggtcgggtg gttcggctgc agccgcgtcg acagccgccc
    21541 gggcaggccg atggtgttgc gaaagcgcgt gaccacgcgg cacttgcgcg cgacgacgat
    21601 cagatcctgc acgcgcatca gcttgctgac cgcggcgacc atctccggcg tcagccccgg
    21661 cgcgagcgcg gcgagcgtgc cggcatcggc ctgcgccgac agcagccagt cgcggaaatc
    21721 accgaccgtc aggtgcgcga ccggcgcgaa ggccgcggca tcgtggctgt cgaggatcag
    21781 gcgcgtgacc tcgtcatcct cgtacgggat cacggcctcg ttcaggaagg tcgcgagcgg
    21841 cagctcggcg agcgcgtact gcgcggcgac gcgctgctcg gccgtttccg cggcgaggcc
    21901 ggcgagcacg tcgccggagc gcagcggcgt ggccttggcg agcaggtcct tcaggtccgc
    21961 gaagcggtgg cgcgtggcgc cgaaggcgtg ggcgtagggc atcgggagac tcctcgcggc
    22021 ggctgcaccg tggctcacgc ccgggtgctg ttgccgccag tctcgccgca tcaggcgacg
    22081 ccatccaccc gtttcatgcc cgccggaaca aggcgccggc ggatggagca gggcaatggc
    22141 aaggcaggag cgaacaagcc cctcccccct tgcgggggag gggttgggga gagggggcaa
    22201 ggtccggacg cagtgccggc gtctccccct ctcccccggc ccctctcccg caaggggaga
    22261 gggggagatc gacgcccggg cgttgggggt cgatccatcc ggcctgcaca gtcgctgact
    22321 cgtgtagatg ccggcgaggg ggaacgggct gaaaagaccg ggtgttcagc cgtgagcggt
    22381 attactcctt gacctcgacc ttggcgccgg ccttgagctt ttccagatag tcgttgacca
    22441 gcttgccctg cagcatctgc gtcagctgcg ggcgcagttc gtcgagcggc ggcggggtct
    22501 ggtcgcgcac gtcttcgagc tggatcacgt gccagccgaa ctgggtcttg accggggtct
    22561 cggtgtactt gcccttgtcg aggccgacca cggcctgcga gaacggctcg accatcatgt
    22621 ccggcgtgaa ccagccgaga tcgccgccct ggtccttgga gccgtccgag gaattggcct
    22681 tggccaggtc ctcgaacttc gcgcccttct tcagctcggc gaccatctgc ttcgccttct
    22741 cctcgctgtc gacgaggatg tggcgcgcct tgtattcctt ggtcttcatg ttcgcgaccg
    22801 ccttgtcgta ctcggccttg atgtcggcct cggacggctg ggcggtcttc aggtatttgc
    22861 gcacgaccgc ggtcgccagc atgttgcggc gcaggagatc gagctgggcc gcgacctccg
    22921 gatcctcggc ggtcttgtcc ttcacggcct gctgcaccag cagctcctgc atgaccagct
    22981 catcgacgat ctgcgcgcgg ctgccgtcgg gatcgagcgg catgccctgc gcggccatct
    23041 gccgggcgcg ctgctgctgg taggtcagga aggtgctctt cgcgatcggc tcgccgttga
    23101 cgacggcgac cggatcgggc agcggcttcg gcgccggggt ggcggccggt gccgccggcg
    23161 gtgcgaccgg agcaggggcc ggctgctggg ccgtggcgtt gccggcgacg gcgagcgcga
    23221 gggccagggc gagcagggcg ggggcgtggc ggacggtggt cgtcatgttg tcagtggggt
    23281 cctgtccgat cggaagcatc tggaaaagtc cggaaagaaa cagacggccg gatcacggct
    23341 cgtccggggt gcgggcatcg atgcccaggg cgtggatgtc ggtctgcatc agcgcgccga
    23401 gcgcggcata gaccgcgcga tggcgtttga cggcattcag accggcgaag gcggggctga
    23461 cgatgcgcac gcggaagtgc ccgccttccc gcgcaccggc gtggccggcg tgctgggcgc
    23521 tgtcgtcgaa gatggcgaag tcgctgaccg gatcaaaggc cgcacgcagg caggcctcga
    23581 tgcgctgcat gcgcccggcg ctcatgccgg cagcaccttg cggaacggat ggatctcgac
    23641 gcccgcgtag acgccggccg cgacgtaggg gtcggcatcg gcccaggcgc gcgcggcttc
    23701 gagcgattcg aactcggcga ccacgaggct gccgacgaag ccggccggac cggggtcgtg
    23761 gctgtcgatc gccggcagcg gaccggcgag cagcagccgg ccttcggcct gcagcgcgtg
    23821 caggcgttcg aggtgggccg gacgcgcggc gaggcgtgcc tccagcgtgc cgggatggtc
    23881 ccggccgatc agggcgtaga acatgcggga tcactccttt cgggcgacgg aatcgcgacc
    23941 ttcgggcagg tggcgcatca gccaccagcc ctgcgcgaac acgaacagca cggtcagtcc
    24001 gagcaggccg aacagcttga agttgaccca ggtgtcgcgg tcgaagtgga acgcgacgac
    24061 gaggttcagc gcgccgcaga cggcgaagaa cgtcacccag gcgagattga gctgacgcca
    24121 ctggcgctcg ggcagttcga tctgggcgcc gagcgcgcgg cgcaccatcg gcttgcgatc
    24181 cccgacgaag gtgctgccga ggaacaccac ggcgaacagc cagttgacgg cggtcggctt
    24241 ccagagcacg aagatgtcgt cgtgcagcca gagcgtcgcg ccgccgagca cgaccatcag
    24301 cccggccgtg accagcgcca tcttctcgac ccggtgctcg cgccaccagg tgtacgcgac
    24361 caccgcgagg ctggcggcga tggcggcggc ggtcgcggtg tagatgtcgg cgagcttgta
    24421 ggcgacgaag aacgcaacga ccggcaggaa atcgaggaac agcttcatcg gggcggcgga
    24481 aggggtgcga cgggccggga acggcgggcg gccagtctat accgaatcgc cgccgccacc
    24541 gtcagcgtgc ccgtgcgggt taagccggcg ttcagccggc ggccggcggt gccgcgccga
    24601 gccgcccggg cagctcgccg gcgctcagcg gctggccgaa cagccagccc tgcgcgagcg
    24661 ggcagccgtg atcgcgcagc agcacggcct cggcctcggt ctcgacgccc tcggcgacca
    24721 gctccagccc gagtgcgcgc gcgagcgcga cgaaggcctc gacgatggcc gggtcgatgc
    24781 gctcgccgcc ggcgtcgaga tagacgctgc ggtgcagctt cagcaccgcc agcggcagcg
    24841 tgcgcagctg cacgagactc gcgcgcccgg tgccgaagtc atcgagcacg agccgcaggc
    24901 ccgccgcgtg cagtgccgcc cacagctgcg gcgacggcgc gccctgctcg gtcagcgtcg
    24961 tctcggtgaa ctcgaaggac agccacgaac cggcgatctc ggcgagcagg cccggcagcc
    25021 gctcgccgtc gcgcagcagc gcccccgaga ggttcacggc catcggcagc gcgccggccg
    25081 gcgtgccggc ccaggcgtgg tgctgcgcgg cggcgctgcg cagcacccag cggtcgatgt
    25141 cgccgagcag cttcacgtcg gcgaccgaga cgaggaactc cgccggtgcc agcaggccgc
    25201 gctgcccgtg gtgccagcgc agcagcgcct cgcagccgac gatgctgcga tcggccaggc
    25261 gcaccagcgg caggaagtgc agctccagcc cgctgccgcc gagtgccgcg cgcagctcgt
    25321 gttcgagcgc catgcgctcg atccacagcg cgttcatctg cggatcgtgg aagcggtgcg
    25381 tgtgccggcc ggcgtgcttg gcctgataca tcgcggcatc ggcgctggcg agcaggacct
    25441 cggcttccgt gccgtcctgc ggacagatgg cgatgccgat cgacacgccg gtgtcgatcg
    25501 tctggccgtc gatcgacacc gggccggtga gcgcatcgtg gatgcgctcg gccagttcgc
    25561 tgagccggcg gccgtcgccg acatcctcgg cgagcagcac gaactcgtcg ccgccgtagc
    25621 gcgccagcgt gtcgctgccg cggatgcagc cggagatgcg cgtgccgatc tcttgcagca
    25681 gcttgtcgcc ggtctcgtgg ccccaggtgt cgttgacgtt cttgaagcgg tcgacatcga
    25741 ggaacagcac cgcgcagcgc gtgttgtccc gccggctgcg cagcagcgcc cgcgccagcc
    25801 ggtcgctgaa caggctgcgg ttcggcagcc cggtcagcga gtcgaagtag gcgatctcgt
    25861 gcatgcgccg ctcggcggcc ttcagctcgg tgatgtcgct gaagatgccg acgtaatgcg
    25921 tgacgtggcc ggcatcgtcc tgcacgacgt ggatgtgcag cagctccggg tacacctcgc
    25981 cgctgcgccg gcggttccag agctcgccgt gccactcgcc gtgcgcctgc agctcgcgcc
    26041 acatctgccg gtagaagtcc tggccgtgac ggccggaggc gatcagcgtc gggcgctggc
    26101 cgatggcgtc ctcgctgcgg tagccggtga tcgccgtgaa cgccgggttg accgccacga
    26161 ggtgcaggtg cgcgtcggtg atcatgatgc cttcgctggt gttctcgaac accgccagcg
    26221 ccagacgcgc gcgctcggcc gcctgcacgc gctcggtcac gtcgttctgc acgccgatga
    26281 agtgcgtgca gacgccgtcc tcgtcgcgca tcggcgagat gttcagctcg ttccagaacg
    26341 gctcgccgtc cttgcggtag ttgcgcagca gcacgctgat ctggcgctgg tcggcgagcg
    26401 cgcgcgcgat ctcggtgatg atgccgcgat cggtctcgcg cccctgcagg aagcggcagt
    26461 tgcggcccat gacctcttcg gccgagtagc cggtgatgcg cgtgaagccg gcgttgacgt
    26521 agctgatcgg gcggtcgggt gcgcgcgcat cacagatgac gatgccgttg tcggccgccg
    26581 ccagcgcccg cgactgcagc gccatctgcc gcacctgctc gcggatgcgc tcctcggcgc
    26641 ggcggatgtc ggtgttgtcc cagaacacca gcaccgcgcc gcggatgcgg ccctgcgcgt
    26701 cgcggtaggg gcgggcgcgc agcaggtgat cgcggtcgcc ggcgaactgg tgcgtcatct
    26761 cgttgccggc atcgatgacg cgctcgacca cctcgcgcag gcccgagaag tcgaggttcg
    26821 cgcgcagtgc gtgcaacggc ccgaccgagt catccgggtc gaggccgaac agccggtcgg
    26881 cggcggggtt ggcgaacatc agccgcaggc gcgcatcgac gaccagcacc gggcagggca
    26941 gcgtgttctt gaggttttcg agatcggcgt tggccgccat cagctgggcg ctgcggctct
    27001 gcagctcctc gttgacggtg atcagctcct cgttggtcga ctgcagctcc tcgttggcgg
    27061 tctccagttc ctcgttggtc gactgcagtt cctcgttggc cgagagcatc tcctcgttga
    27121 gcgactgcag ctcctcgttc gaggtttcga gctcctcgat gacggtctgc aggtgctcgc
    27181 gcgaggcggc cagctcctgt tcgagttcga gcgtgcgctg catctgctcg gactcggcgc
    27241 cggcccggcc ggtctcgatc tcggcgcgcg gatgctcgaa gcagaccagg aacagcgggt
    27301 cctcggggaa gtcgccggcc agcggatgca cggccagacg cacggccggg ccgccgtcca
    27361 tcgtgatgcg gcggctggtg gtcagcgcgt tgctgctgcg gcacttcagg atcaggccgc
    27421 gcaggtcgac gtgcagctcg cgccggatca gcgtcgtgat cgacaggttg gccttgcctt
    27481 cggcgaggcg gatgtacggc gacacgtcac cgagcgtgta gcgcacctcc atggcctcgt
    27541 cgacgacgat gccggccggc gcgtaggcgg cgagcgcgac cagccgcacg cgctcttcga
    27601 gcgtcatgcc ggcgcgcgcc ggcgggcgtg tggcgcggtc gttctcgcgc accggcacgc
    27661 gcggcatctg caggctgcgc gcgcgggcgg cgtcgcggcg gcggaacagc ttgaccttgc
    27721 cggccgaggg cgcgaacaga tcggcggtat gcgggttctc ggacttgccg agcagcagcc
    27781 agccgccggg gcgcagcgcg tagtggaaca tgtcgagcac gcgctcctgc agcgggctct
    27841 tgaagtagat cagcaggttg cggcagctga tcaggtcgag acgcaggaac ggcgggtcct
    27901 gcgcgaggtc gtggcgcgcg aagatcacca gatcgcgcag cagcttgccg acctggtagc
    27961 tctcgccggc ggtcgtgaag tagcgctcca gcagcggcgg cggcacgacg ctcgcgacgc
    28021 tgtggtggta gacgccgcgg cgcgcgatcg cgagcgcatt ggtgtcgagg tcggtcgcga
    28081 agatgtgcac cttgcgctcg ccgaggcgcg tgccgagggc ctcggcgagc aggatggcga
    28141 tcgtgtacgc ctcctcgccg gtcgcgcagc ccggcaccca gacgcgcagg tcatcgtcgg
    28201 gggacttctc ggcgaccatc tcgcgcagca cgccggccag cgcctcgaag gccggcacat
    28261 cgcggaagaa cgccgtcacc gagatcagga tgtcctgata cagccgctcg atctcgcccg
    28321 actgggtggc aagcagcatc tcgtagtctt cgagcgtcgc cgtgtgcgtc gcgagcatgc
    28381 gtcgcgccac gcgccggcgc agcgtgttcg gcttgtagtt caggaaatcg gtgccggtgt
    28441 ggcgctgcac cagcctgagc atgcgctcga acaccgaggc cggcacgtgc ggcgagccga
    28501 ccggcagacc accctggacg ccgagctgcg ccaggcgcat cagctcctcg gcgatgcgct
    28561 cgggcggcag caccagatcg acgcagccgg tggcgatcgc cgccgccggc atgcccgagt
    28621 acttggcgct ggcatcgtcc tgcgcgaacg tgaagccgcc ggcggccttg accgcgcgca
    28681 cgccctcggc gccgccggtg ccggtgccgg agaggatcac ggcgatggcg ttctcgccct
    28741 ggtcctcggc gagcgcgtgc aggaagtgat cggccgaggg cttgggcacg cccgggtcgc
    28801 tcgcctcggt cagcaccagc cggccgtcat ggatcgagac gttcgtgcgc ggcggcgtca
    28861 cgtagacggt atcggcgacg atctcctgcc cgtcgctgac atcgacgacg cgcagcgcgc
    28921 attcgcgctg cagcagctgc accagcaggc tcgggtgcga cggcgacagg tgctgcacga
    28981 ccacatacgc cagccgcccg gtcgccggca gcggccgcag cagcccgccg agggcttcga
    29041 gcccgccggc cgaggccgcg atgccgacga tcagcggcgg acgcggcccg gaatcggcgc
    29101 cgtcttcggt ctcgtcccgt tcggcgtccc gttccacgtg cgcagccgga tcatcggccc
    29161 cggccttcgg gtcgtcttcg gattcctgca tggctgaatt ccggttgttc ttcgctggat
    29221 tccgggcgct gacgggctgc tgacagaaac cctgcggccg gtcatttgac cgcccgcgcg
    29281 tggcggtggc aaggtgcgcg ccgcttctta cagggtcggt ccgtccatgg gcgagcgcat
    29341 cgagatacat ccggtcaatc cgcagccgcg cctgatccgg cgggcggtcg aggtgttgcg
    29401 cgagggcggc gtcatcgcct acccgaccga ttcctgctat gcgctcggct gtcgcatcgg
    29461 tgacaaggat gcgcagaccc gcatccagcg tatccgccag atcgatgatc gtcgtcataa
    29521 tttcacgctg atctgccggg acctgtcgga gctgagcctg tacgcgcgcg tcgacaacgc
    29581 ccagttccgg ctgctgaagg cgctgacgcc ggggccgttc acgttcatcc tgcgcgccac
    29641 gcacgaggtg ccgcggcgcc tgcagaacga gaagcgcaag accatcggcc tgcgcgtgcc
    29701 gcagcacgtg atcgtcgcgg cgctgctcgc cgaactcggc gaaccgatca tgagcgcgac
    29761 gctgatcccg cccggcgagg accgcgcggt cagcgatccc gaggacatcg agaccggcct
    29821 cgggcgcgag ctcgacctga tcgtcgatgg cggcaactgc ggcatcgaac cgacgacggt
    29881 gctcgatctg gtcgagggcc tgccgccggt gctgatccgt cagggccgcg gcgacgccag
    29941 cgccttcctc gccgaagcct gaagcggctg cgcgggacgg catctgctgc agcgcaacgc
    30001 cgttgtcagc gtccggcctg cgccccgtat actcgaccca tgcccgaact caacgacatc
    30061 cagcgcctgg ccatcatggc cctgccgatc ctgttcgcga tcaccctgca cgaggtcgcc
    30121 cacggctggg cagcgctcag gctcggcgac accaccgcct ggcagcaggg ccggctgacg
    30181 ctgaacccgc tcgcgcacat cgatccgatc ggcaccatca tcgtgccgct ggtgctgtac
    30241 ttcacggccg gattcatctt cggctgggcc aagcccgtgc cggtcagcat gcaccggctg
    30301 ccgaatccgc gccgcgacat ggccttcgtg gcgctggccg ggccgctggc gaacttcgtg
    30361 atggccaccg gctgggcgct ggtcatggcg ctcggcgcat ggctttcgca cgtgctgccg
    30421 gaactcggcc agccgctgtt cttcatgggc tcggccggca tcggcgtcaa cgtcgtgctc
    30481 gcagtgctga acctcgtgcc gctgccgccg ctcgacggct cgcgcgtgct cgccgccgtg
    30541 ctgccggcgc gcgtcggcgt gcagctctac cgcttcgaac aggccggcct gatcctgctg
    30601 gtcgtgctga tggccaccgg cgtgctcggg cgcgtcatcg gcccgatcgt cgaggccgtg
    30661 cagcagttct atctctggat cgtcgtgagc ctgctcggct gatccctgcc gcctgccgcc
    30721 gctgcagccg gtcaccgccg gatgcccgct ccggacccgg ccttgctccc ttcacagggc
    30781 cggggccggc gagaggggga tgagaaaacc ctgcagtcct gccggtcacg gcggaacgcc
    30841 cggacttttt cagcccctcc ccctttgcgg gggaggggtt ggggagaggg ggatgcaccc
    30901 cgcattcatg cgtgatcggc atcccgtgtg atcggcctga ccggcctgac ccgaaatccc
    30961 gcgccgctgc cttgtccgcg gccgcagccc tggaccgcct cccgatgagc aagacccgca
    31021 tcctgaccgg catcaccacc accggcaccc cgcacctcgg caacttcgcc ggggcgatcg
    31081 gcccggcgat cgaggccacg cgccgcaatc ccgaggccga attcttcttc ttcctcgccg
    31141 actaccacgc gctgatcaag tgtcaggagc ccgagcgcgt gcagcgctcg cgcctcgaag
    31201 tcgccgccac ctggctcgcg ctcggcctcg accccgagcg cgtctgcttc taccgccagt
    31261 ccgacatccc cgagattccc gaactgacct ggctgctgac ctgcgtcacc gccaagggcc
    31321 tgatgaaccg cgcccacgcc tacaaggccg cggtgcagga gaacctcgat tccggcgacg
    31381 ccgaccccga caagggcgtc acgatggggc tgttcagcta cccgatcctg atggccgccg
    31441 acatcctgat gttcaacgcc cgcaaggtgc cggtcggcaa ggaccagacc cagcacctgg
    31501 agatggcgcg cgacatcgcc cagcgcttca accacatcta cggcgagcac ttcgtgctgc
    31561 ccgaggccgt ggtcgaggag gatgccgccg tgctcgccgg gctcgacggc cgcaagatga
    31621 gcaagtccta caacaacacc atcccgctgt tcgtgagcgc gaaggagctg cgcaagctgg
    31681 tcatgcgcat cgtcaccaac tcgcagctgc ccggcgaacc caaggacccg gacggctcga
    31741 cgctgttcca gatctatcag gccttcgcga gcgccgacga aaccgcggcc atccgcgtgc
    31801 gctacgccga aggcatcgcc tggggcgaga tgaagcaggt gctgttcgag aagctcgact
    31861 ccgtgctcgc cggcccgcgc gagcactacg cggcgctgat cagcgacccg gcggcgctcg
    31921 aacgcatcct gctcgccggt gccgacaagg cccgcgccat cgccagcccc ttcctcgccg
    31981 gacttcgcgc cgccaccggc ctgcgcaagc tcggctgaac ccttgacccg gccgggcggc
    32041 gcatgacgga aggactctcc ggcaccgccg gcgacgccac ggatgcgccc ggcccggctg
    32101 cggctgcgcg catccgcggc gtcgcgctcg aacgcctgcc cgaagacctc tacatcccgc
    32161 ccgatgcgct cgaagtcctg ctcgatgcct tcgaaggccc gctcgacctg ctgctgtacc
    32221 tgatccgccg ccagaacctc gacctgctcg acatcccggt cgccgagatc acccgccagt
    32281 acctcgccta cctcgaactg ctgcacgaac tgcgcttcga actcgccgcc gaatatctgg
    32341 cgatggccgc atggctcgcc gagatcaaat cccgtttgct gctgccgcgc ccgccacgca
    32401 gcgacgagga cagcgaaccc gaagacccgc gcgccgaact cgtgcgccgc ctgcaggaat
    32461 acgaacgcta ccggcgcgct gccgaggacc tcgacgcgct gccgcgcctg gagcgcgatg
    32521 tcttcgccgc gagtgctgcg ctgccgcgcc acgagcgcgt gcgcgaaccg ccgcacgtcg
    32581 aactcggcga actgctcggc gccctgcgcg aggtgctcgc ccgcgccgag ctgcacgcca
    32641 gccaccgcgt cggccgcgaa ccgctgtcga tccgcgcgcg catgagcgaa atcctcgtgc
    32701 gcgtcgatgc cgtgcagttc acgcgcttca ccgaactgtt ccacgccggg gaaggccgcc
    32761 tcggcgtggt cgtcaccttc atcgccgtgc tcgaactcgt gcgcgaggcg ctgctggagc
    32821 tggtgcagag cgaagcgttt gcaccgatcc acgtgcgccg gcgggcctga gnnnnnnnnn
    32881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtcatccc gagcgcatcc ccagtcatat
    33001 ctggtgcatc cccagtcata tcgagcacat ccccagtcat atcgagcgca tccccagtta
    33061 catcgagcac atccccggtt gtaccgagtg catccccagt catatcgagc gcatccccag
    33121 ttacatcgag cgcatcccca gttgtaccgg gcgcatcccc agttacatcg agtgcatccc
    33181 cggttgtacc gggcgcatcc ccggttgcac cgaacgcatc cccagtcata tcgagcacat
    33241 ccccggctgc accgggccca tccccggtgg ggacgagcgc atccccgcct ttgtcgtacc
    33301 tgcgccgcaa gcatgcgtgc cgatcacgat gcccatcggc cgctcagccc gatcttccgg
    33361 cagtcgccgg cagcggccgc agccctgctt gcccgtcgcg caccagcatg aagaaaaaat
    33421 catcttccgc cttgcggaaa gcggcatgac cgtattccac actgccgcga caaggctcgc
    33481 agccttccgc cccgaaggcg cgatgcccgc aggtcgccac ctgcctttcc gtcggggcag
    33541 ccagggatca caaggagtcc gaaatggcca gaaatgcctt tgccgaccgt gaagccgcct
    33601 tcgtccgcgc cagccaggcc tggaagcgcg acgatcccga aaagctgttt gccggcctga
    33661 cccagcaggc ctttgccgat caggtcgccg aagcccagcg caccagtcgc cagctcgaag
    33721 ccctgctgag ccaggtcgat cacctgcgca accagcgctc cgcccagttc aagaaaatcg
    33781 ccgccctgaa cctgcgcctg aaaagcgcca tcgtcgccga ccccgatcac ggttccgaca
    33841 gcaccttgtt cgaagccttc ggcggcatcc gcgtcagtga gcggcgcagc ggcttgacgc
    33901 gcaagcgcaa tgattcgacg gatgacaaga cggggacgtg ataaggggga ggtggcagat
    33961 cgcaggagat atcggggatt gtgcatgcat agtgttgcaa gactggttat ggcgcgcggt
    34021 caggatgagg agttttatta cgtcattaga ttgcgcgcct gatcagtcct atgatctcgc
    34081 gtccagtgat gtgggagagt tgggtggcgc agtccggtca gattcaccta gttagggctt
    34141 gtagtacaaa atgctttaat gtttaggaag agtgtgcgct ggagtggcgt ataaagccaa
    34201 ggtgttattc agtacgaggt ggatgattat gggaactcaa agttgttcta gctgtaatgg
    34261 taggggtgta cgtgaagaac tggtatcaaa accatgttca tcgtgcggtg gaagcggtag
    34321 caaatatacg agttatggct ctcaagcttg tatggcttgt atggggtcag gtacaaaata
    34381 tgtctatatg tgtgttgatt gctcaagttg tggtggttct ggtttagtct gggtttcgga
    34441 ctactaaatt atctagagcc gtagggcgga ttaagcaagc gtagggcgga tcaggcgcgc
    34501 attgcggggt atatagaatc atgatccttg ccgcgcgccg taatccgcct ttcgcgttta
    34561 ctgcaagccc tcggagagcg ttgccaaacc at
//
LOCUS       DAOQIE010000003        33024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_556_length_33024_cov_7.517729, whole genome shotgun sequence.
ACCESSION   DAOQIE010000003 DAOQIE010000000
VERSION     DAOQIE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 33024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33024
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_556_length_33024_cov_7.517729"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1886
                     /locus_tag="PK144_00325"
     CDS             93..1886
                     /locus_tag="PK144_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNF64196.1"
                     /translation="MSDLAEFVVILTAIVLAVPLARRCRLGAVIGYLIAGVVIGPSGL
                     GLIRDHEAILHIGELGVVLLLFLIGLELKPSRLWALRRPVFGLGGVQVLATGAVATLA
                     LRLLDYPPAGALVAGLALSLSSTAFVLQLLAEQGELGSRHGRDAFAVLLLQDVAVIPM
                     LALLPLLATEARPGDEGDGLRQAAVAVAAITVAVLASRYLVPRLFRLIAASRSHEVAT
                     AGALLVVAAAALGCAEIGLSMSLGAFLIGLLLADSEFRHELEANIEPFKGVLLGLFFI
                     AVGMSANLGLLAEAPLRLLGLTLGLMALKAGLMFGVACAWGHAAASAGRVAVLLAQGG
                     EFAFVLFANAAGLGLLDAARVEELSVVVTLSMALTPLLLAALQAGEHWLAARRPRPPG
                     PPLPPISPAPVLIAGYGRFGQTLARFLRVRHVPYTLFDTDVARIELGRQLGETVHYGD
                     ASRVELLRAAHAAHAAVLVIGIDDVETSLRAAATAHRHFPQLRVLSRACNARHTLRLM
                     DLGVQAVASDTYLSALEMAECVGVALGDAPADIRAEVARFREHDQRNLRRLHGLHHDE
                     RRMLAAARAAVLEMEAVFGGRERRPPAGRDD"
     gene            1999..3237
                     /gene="ispG"
                     /locus_tag="PK144_00330"
                     /gene_synonym="gcpE"
     CDS             1999..3237
                     /gene="ispG"
                     /locus_tag="PK144_00330"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNF64197.1"
                     /translation="MSQSSSASALPRRRAVRVQVGSVAIGGTAPVVVQSMTNTDTADA
                     VRTAIQVAQLARAGSELVRITVNTEEAAAAVPEIRARLDDMGLAVPLVGDFHFNGDRL
                     LAKYPACGHALGKWRINPGNVGRGSRRDEKFAAIVRLAVEMEKPVRIGVNWGSLDPAV
                     LARLMDENHRRPEPWDAATVMHEALVASALESAGAAEALGLAHERIVLSAKVSGVQDL
                     IAVYRKLAARCDYPLHLGLTEAGMGSKGIVASTAGLAVLLQEGIGDTIRISLTPEPGA
                     AREQEVIVAQEILQCMGLRSFTPMVISCPGCGRTTSDYFQRLAQQIQSYLRAQMPLWR
                     AEHPGVEAMTVAVMGCVVNGPGESKMANIGISLPGADEDPVAPVYVDGKKTVTLKGDT
                     IAADFQAIVDDYVKSRFTRT"
     gene            3326..4114
                     /locus_tag="PK144_00335"
     CDS             3326..4114
                     /locus_tag="PK144_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64198.1"
                     /translation="MKIQDLTVLLVEPSATQAKIVRQQLQALGVTRIETVTSGAAALP
                     VVQARDLHLVVSRLYLPDMSGADLSKTINALDRPFRPAFMLISSETNEAYLEPLRQAG
                     AAAIVRKPFDASFMQEALHAVLDHLEPGTLHLGERDIEEIRVLLADDSTAVRRFLVQL
                     LRGLGIDRIDEADDGATAMPFIDQHYYDLLITDLHMPLLNGIELTRYVRCESQQRSLP
                     IVMITSEADGGRLDMARAAGVSAFLHKPFEAKALKAHIERLLAG"
     gene            4284..5159
                     /locus_tag="PK144_00340"
     CDS             4284..5159
                     /locus_tag="PK144_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNF64199.1"
                     /translation="MNDRRPPDDDPLLQRLGEQLERRGVHAGTETARALLERASAELR
                     RGADLDQLAARLAEQACAAARRVSSSAAQPVERLIAVGASTGGPDAIRTFLRGLNRRS
                     PGVVIAQHMPAGFTRLFAERLTQTTGLEVIEAAGNESILPGHVYIAPGHAHLTVRRAG
                     SHFLTRLSDAPPVNDVRPSVDVLFESLAGCAGEHVTGVLLTGMGRDGAQGLLAMRQAG
                     ARTFAQNEATCTVYGMPRAAVRIGAAETVLPIERLAAAVLGEGAGGISPFEASPRSAV
                     ATGSFRAAVPAFGCV"
     gene            complement(5077..5889)
                     /gene="nudC"
                     /locus_tag="PK144_00345"
     CDS             complement(5077..5889)
                     /gene="nudC"
                     /locus_tag="PK144_00345"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNF64200.1"
                     /translation="MLQLPDRYVAAVPAADPAAPGWWFALQDGRLLVLRAAAGASLPF
                     GRDAPLPGSVLGLGHFDGVPCRALAVPPEAGPLPGAAFVSLRALWGQLDPELLGIAAQ
                     AAQLLTWDATHRFCGVCGTPTESRTDERARACPACGHMSYPRVSPAVMVRVTRGEEIL
                     LARSPRFAPGVWSILAGFVEAGEALEQTVTREVREEVGLEISGLRYFASQPWPFPHSL
                     MIGFTAEWSGGEIAIDGVEIIEAAWFTRSRMPGLPHEMSLSRRLIGDWLQKG"
     gene            complement(5954..7720)
                     /gene="dauA"
                     /locus_tag="PK144_00350"
     CDS             complement(5954..7720)
                     /gene="dauA"
                     /locus_tag="PK144_00350"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HNF64201.1"
                     /translation="MKPTEEHSGGPRFAVALRETLRAGYGVSDLRADLLAGITVGIVA
                     VPLAMALAIGSGVPPQYGLYTAVIAGLLIALTGGARFSVSGPTAAFIVILHPIAEQYG
                     MGGLVLASLMAGVMLLGMGIGRMGKLIQFIPYPVTTGFTAGIGVVIASLQFKDFFGLS
                     FAHAPESFFERMHQVGLALPGLNPADLLVGAVTLAVLILWPKLRTPIPQHLVALAVGT
                     LLAYGLERLFHVEVATIASRFSYTLDGETLHGIPALLPQFVLPWNLPGADGAPLGLTL
                     ATVKSLVGPAMAIALLGAIESLLCAVVADGMAGTRHDPDAELIGQGLGNLIAPFFGGI
                     AATGALARTATNIRAGARSPLAAAIHALFILLVLIALAPLLGYLPMSALAALLLLVAW
                     NMSEMKHFLHITRVAPKSDIIVLLVCFGLTVVFDMVVAVGVGVVLAALLFMNRMAEFA
                     GMRLQGEETHAHAGALPAHTRLYEVAGPLFFGAAEKAMSALHRIDDHCEVVILDMESV
                     PVMDISGVVALDSAIRRLHADGICVVLAGVRGQPAKVLARAGIEEVPGRLLIEGKVST
                     AIWRVRRLLGLEDTASVALTEL"
     gene            8122..9147
                     /locus_tag="PK144_00355"
     CDS             8122..9147
                     /locus_tag="PK144_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF64202.1"
                     /translation="MRKSSHSAAVVTAALLAFAGTAHAGATLDAVKKKGFVQCGVSDG
                     LPGFSYTDDKGNVSGLDADLCRAVAAAVFGDATKVKFTPLTAKERFTALQSGEIDILS
                     RNTTWTSSRDTALGLNFTGVTYYDGQGFLVNKKLGVKSAKELDGASICIQAGTTTELN
                     LADYFRANNMKFTPISYDKSDESAKAMEAGRCDGLTSDQSQLYAQRIKLAAPDDYIVL
                     PEVISKEPLGPVVRHGDDEWFDIVKWTLYVQVAAEEYGLTSKNVAEAAKTSTNPDVKR
                     LLGAEGEKGKDFGLANDWVVNVVRQVGNYGEMFNRNVGDGSPLKIKRGLNALWNQGGL
                     QYAPPFR"
     gene            9294..10481
                     /locus_tag="PK144_00360"
     CDS             9294..10481
                     /locus_tag="PK144_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNF64203.1"
                     /translation="MTTPLAGAAPARPPFWNDPAVRALVFQALALIAVIALGWYLVHN
                     TQQNLAQRGIASGFGFLNNAAGFGIVQHLIDYSPADSYGRVFWIGLLNTLLVSVLGIV
                     FATVLGFVVGIARLSRNWLVAKIAMVYVEIFRNIPLLLQIFFWYFAVLRTVPNPRNSL
                     AFGDAFFLNIRGLYVPRPVFGDDFVWVLGALALAVVLAVLLGRWARQRQATTGQPFPV
                     FLVSLAILVVLPGVTYFAAGQPLTWDIPKLAGFNFRGGWVVIPELAALLVALTLYTAT
                     FIAETVRAGILSVSHGQTEAAGALGLPNGLTLRLIVIPQAMRVIIPPLTSQYLNLAKN
                     STLAAAIGYPDLVATFAGTVLNQTGQAIETIAITMSVYLALSLGISMLMNWYNKRKAL
                     VER"
     gene            10484..11602
                     /locus_tag="PK144_00365"
     CDS             10484..11602
                     /locus_tag="PK144_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003216325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNF64204.1"
                     /translation="MTEPNPPAATFTPHPDLPPPAATTGVIGWLRANLFSSPLNALMT
                     LLVAAALLRWIPPILDWLIFSADWSGSSRQDCTGEGACWVFIKQRLGQFIYGFYPEDQ
                     RWRVNLVWVIAVVASWPLFLKRNRLQVPAGLFMLFVFPVIAYGLYTGPLFGLSHVETS
                     QWGGLMLTLTIAVVGIAGSLPGGILLALGRRSKMPVIRTLCVTIIEFWRGVPLITVLF
                     MSSVMLPLFLPEGMSFDKLLRALIGVVLFESAYIAEVVRGGLQAIPKGQYEAADALGL
                     NYPKAMGLIILPQALKLVIPGIVNTMIALFKDTSLVIIIGLYDLLNSIQQAAVDPNWL
                     GFATEGYVFAALLYWVFCFSMSRYSQHLERRLHTGHKR"
     gene            11631..12407
                     /locus_tag="PK144_00370"
     CDS             11631..12407
                     /locus_tag="PK144_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNF64205.1"
                     /translation="MSDTASSPKLVIGDKVMIEMKGVHKWYGQFHVLKNINLNVRQGE
                     RIVLCGPSGSGKSTTIRCINRLEEHQQGQIIVDGTELTSDVKHIEAIRREVGMVFQHF
                     NLFPHLTVLENCVLAPIWVRKMPRREAEEVAMQYLRRVKIPDQAKKYPGQLSGGQQQR
                     VAIARSLCMKPKIMLFDEPTSALDPEMVKEVLDTMVSLAEDGMTMLCVTHEMGFAKQV
                     SNRVIFMDRGEIIEQNDPHEFFNNPQNERTRLFLSQILHH"
     gene            complement(12482..13558)
                     /locus_tag="PK144_00375"
     CDS             complement(12482..13558)
                     /locus_tag="PK144_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF64206.1"
                     /translation="MSPGQIWQMGRQAVSAWSDDYAASMGAALAYYTTFSIAPLLIIV
                     IAIAGAVFGHDAAQGHILAQLRDLLGKDGALAIEWLLKSASDPQQGMVATVISVVTLV
                     IGATTVFGELQTSLDRIWQAPAATRPEGLWALLRTRLLAFGMVLGIAFLLLVSLVLSA
                     VIAALGEWWSPWFGERELLAQAANFIAGFVLITAAFALIYKLLPRARIAWRDVWTGAA
                     ITALLFSIGKLLIGLYLGKSSTASAFGAAGSFVVLLVWVYYSAQIFFLGAEFTWVYAH
                     AHGSRRGEPRSRPAPAPGGRELPQRNRPAGPAVRAPAGAGAQALGPLPDTRFSPLLRT
                     AAVVGAAWAVLTVTAGGRGAGPRR"
     assembly_gap    13997..14096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14437..14658)
                     /locus_tag="PK144_00380"
     CDS             complement(14437..14658)
                     /locus_tag="PK144_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64207.1"
                     /translation="MHPARRLAFITLNGLLAGLGLFLALNLLPELRGGDALAWLASGT
                     AKVAFVRELCFGFVMAWALRPRATTEADA"
     gene            complement(14836..15459)
                     /gene="rhtB"
                     /locus_tag="PK144_00385"
     CDS             complement(14836..15459)
                     /gene="rhtB"
                     /locus_tag="PK144_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009848221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HNF64208.1"
                     /translation="MDLHVWLAWLAACVLISISPGAGAVNTMSNALSYGRRRTFIGIL
                     GLQGGLIVNTLLVGVGLGALIAASATAFTAIKWAGVAYLLWLGWQKWHAPTHFDVSEK
                     SVALPGPARLFWQAVIVNVTNPKAIVFLVALFPQFLDPARPQGMQIALLGGTLVVVDV
                     IVMCGYATLAGVLRRHVQNARRMRLLNRVFGGMFAGAGALLASVRHA"
     gene            complement(15595..16182)
                     /locus_tag="PK144_00390"
     CDS             complement(15595..16182)
                     /locus_tag="PK144_00390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64209.1"
                     /translation="MSALNFSLRPARVEEAAIVAGAIAEASGGYAPLAWSALAGDGDL
                     HATGARCCAADDGPHSWRNALVLETQDGAVAGVLLGCAAAQVPPVAAVLGADGSDLCA
                     PVRMQVPDTWLLVGMTIFSPWRNYGLGHRLLVQACSQARAAGFDRVSLIACEHNPATR
                     LYRREGFTVVERRPILPHPLLAYAGDVLLMVRALP"
     gene            complement(16202..17044)
                     /gene="queF"
                     /locus_tag="PK144_00395"
     CDS             complement(16202..17044)
                     /gene="queF"
                     /locus_tag="PK144_00395"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831211.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNF64210.1"
                     /translation="MSDLSHLPLGRFVAGSEHYDPALLCTVPRAGQRAQLGFSDNTLP
                     FVGVDLWNAYELSWLTPAGKPQVAMASFAVPAESPNLVESKSLKLYLNSFTQTRLRDR
                     TDLLRRLSADLGAAFGTLIGVHLTGPEAFAAQRSEELAGQCIDELDVSPESYGPPDPA
                     LLMLATGVPRKPVEETLVSHLLKTNCLVTGQPDWASVQIRYTGQPIDRAGLLKYLITF
                     RNHHEFHEHCVERIWRDIHERCQPESLAVYARYTRRGGLDINPFRASHSARPWPENLR
                     TARQ"
     gene            complement(17206..17772)
                     /locus_tag="PK144_00400"
     CDS             complement(17206..17772)
                     /locus_tag="PK144_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNF64211.1"
                     /translation="MGERSGLLRADLRIDDLDRGHHGVYAVRLARHPSETAERLMIRL
                     LAFIGHADTALNFGRDLCVAGEPALLQTAADGSPALWVEVGLPGARRLLQAAGRAPRV
                     SVWAFGGRAAEAWWQRLPARVQALPGLEVRLLAAAETAALAARMARTMRFDCRLQHGR
                     CQLTDAAGTLTLQLQPRPAGGTRPAPSA"
     gene            17959..21615
                     /locus_tag="PK144_00405"
     CDS             17959..21615
                     /locus_tag="PK144_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64212.1"
                     /translation="MLHASSLLPPLTPALRGRLALLAAGYGVFAVVAILLSRQPGSLA
                     TVWFANAWALVFWLRSRQVLPRRLQALAVLVAAWIANYVCNTPATLSLAFAGVNLAEI
                     VFGGLLIERHARERPESAGAMLRLLVFGVFVPPLLGATLGALLLQIAGIAEAVPVWPL
                     WYCGSVIGALALLPLGVTATRAWGDARGRLETLAGVLGAALVAGLVLPNLAFPYVYVT
                     LPLVLAAMRLPVAGTALAVCVTTAVWALLLAFERIPASEGGPVWTLLTPMIVTLLPAL
                     LLAAAVAETRMQTRRLANRERFVRLVTDALPGMVGYWDSGLRCRYANRAYREWFGREP
                     ESLYGETMQSLLGPALFAKNEPYVRAVLRGEPQRFERTLVKADGSTGHTWAHYIPDCP
                     GPDRPGSGRPDGVNGPVAGFFVLVTDVTELKAAELALARLNAELAERSVQAEAANRAK
                     SEFIANVSHEIRTPMNGILGLLQVLEDTALDSRQRRYLRTIRQSARALLDILNNILDH
                     SRIEAGRLDLHREDFDLPELLEGVVDLFAPAAHQKRLELLLDAPAGLPARLTGDALRL
                     HQVLVNLLGNALKFTERGEICLGVERLPGAAPGCARLRLQVRDTGIGMNEAQLQRLFT
                     PYVQADGSTARRYGGTGLGLVISQQLVRLMGGEITVSSAPGAGSTFGFELELPLAGPP
                     VRSLAPVPGRLAGVRVLLADAGATARGIVGRALEHWGCDCVEAHDADALLDTALSAAG
                     SGRPFRFCLIDFRLPPQGALKAQQRLLAERNAGRLPASTTILLLTEGERLELDGEPLA
                     AGLPVLMKPVTASRLHDVLIEHLDHELGIDNTSVPAPGEDWHRLGAVLRGAQVLVVED
                     NAVNRMVAEELLERLGIRVTAAADGGSGVELALAGRYDAVLMDLHMPGMDGFEATARI
                     RADGRHDALPIIAMTAAVFDEDRRATAAAGMNDHLAKPVERDELIRVLRRWIAPRIST
                     APVPFPPAATEVDAMSGARPVVPGAAVPADLPAEAGDEALPVLPGFDLDSACLRLGGD
                     RALLRRLMQTFASGYADAGVRLRAAFAQPARSELVRLLHTLKGASGSIGATGLQAESA
                     RLETLLRGGGEPGDIEALSTLLDATLATLARYLDETATPAAAAAVPVPPPASATLSAS
                     LRELQGLLESHRLVPDALVARLRSDCGGHEVAIKLLTAVDNFDYEQASRYLELLITEY
                     EHDGLGTSGPSRPS"
     gene            21575..22558
                     /locus_tag="PK144_00410"
     CDS             21575..22558
                     /locus_tag="PK144_00410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF64213.1"
                     /translation="MTAWGPPDHHDLHEQPPRVLLVDDAPINLQLLASALGRGYELHV
                     ARDGAAALDCIRREPDLDLVLLDIHMPGLDGFEVLTWLREEFPERRLPVIFVTAAGDE
                     QAQTDGFALGAVDYIVRPFSLPVVRARVQTHVALKRQTEHLERLAWLDGLTGAANRRS
                     FDQRLAAEWRRALRNRTPLGLVLFDLDHFKAYNDRHGHPGGDDCLRRIAALGQQSIRR
                     PGDLIARYGGEEFALLLPDTDLAGVHGLAERLRRRVETCFACSGGDCNVRGAPGPMTL
                     SAGCASRVPGFNEDPMTLILEADRRLYAAKVAGRNRVVATDDALPAAGGVA"
     gene            22555..24723
                     /locus_tag="PK144_00415"
     CDS             22555..24723
                     /locus_tag="PK144_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64214.1"
                     /translation="MTAQVDVTRHHRPRVLVVDDAPVNRLVLSEALGEDYEVLLADDG
                     PGAVRLAGGEARPDLILLDVNMPGMDGYAVCEQLKESTRTRDIPVIFVTARNEPDDEV
                     RGFTAGAADYITWPLQLPVVQARVRTQLSCSGLIQRLDAQNRVLAERLAALADVGGEV
                     PARLYLRALEHTRDAVMITRADSTIVAVNHAFAALTGYPESEALGRTPRLLRSGRHPP
                     AFYKELYATLAARGRWSGELINRRRDGSLFPAVMTIAAVHDRDGELGHYVSAFSDVTM
                     IRQVQERMDYLTWHDALTGLPNRLLLTDRLRQTLKLGQCDTRVAVAMVLDIERFGALN
                     EALGAQVADAVLGVVASRLRRELCTGDTLARLDGDEFAVVLAGVYDSRESAAHHARLV
                     AGQLGAAVAEPLEIAGSVLRLGLGIGITLFPKYENDEAGEVLRRAQTARHRAGDPETG
                     GQSIVFFDERMGESVEHRFRLEQELHHAINAGELRVYLQSQVDQDGRITGAEALLRWQ
                     HPQRGLVPPGEFIPIAECTELIVQLDRWVLGEVVALLARSAGCRPAPTLSVNVSPRMF
                     ASPGFVDELRTLLAPHPDSARRLVIELTEGVLVHDPEVVAERMAALGALGVRFSIDDF
                     GTGYSSLTYLKRLPLHELKIDRSFILEAPHDADDAALVGLIVSVAGHLRLEVVAEGVE
                     TAVHADWLRSHPGVRRQGYLYGRPVPAGDWFGDYASGCDA"
     gene            complement(24813..25028)
                     /locus_tag="PK144_00420"
     CDS             complement(24813..25028)
                     /locus_tag="PK144_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HNF64215.1"
                     /translation="MKNLIKLSALAGLSVLAVGCASTSDLEAVRAEAQSAKATANQAL
                     ATANEAKSIAMDTNEKLSRMFKKSMYK"
     gene            complement(25188..25538)
                     /locus_tag="PK144_00425"
     CDS             complement(25188..25538)
                     /locus_tag="PK144_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 pili tip component"
                     /protein_id="HNF64216.1"
                     /translation="MHIRNLIREWERAGVRTLTVESCCVQLPLHDAARLAALAEMYPL
                     KSRAELITELLSAALDQVEAAFPYVQGSRVIAEDEQGDPMYEDAGLTPLFLRLTKRHA
                     ARLAEESAQQKDGA"
     gene            25880..26191
                     /locus_tag="PK144_00430"
     CDS             25880..26191
                     /locus_tag="PK144_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64217.1"
                     /translation="MLPLWFDMNARVWLHAQHVLLDNVVAMMRLVSGARCPAPVRLAD
                     RCDGCGGALQWRGPQAAPYQRCPACGLIRFHAPLPQLPAGSAISGETHRVLSFRPRRG
                     A"
     gene            complement(26210..26662)
                     /locus_tag="PK144_00435"
     CDS             complement(26210..26662)
                     /locus_tag="PK144_00435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF64218.1"
                     /translation="MTDAHRAVVRWQRATADFDAKTFERTHTIVYDGGEQVRASTAPE
                     YGGDASAVDPEQGLVGALASCHMLTFLTIAALKRIVVDAYEDEASAETGRNADGRMMV
                     TRVTLRPRVRFAPGHEQDASALDALHARAHGNCFIANSLRSVVSIEPR"
     gene            complement(26698..27318)
                     /locus_tag="PK144_00440"
     CDS             complement(26698..27318)
                     /locus_tag="PK144_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNF64219.1"
                     /translation="MYGLDQPVLRTDISGMPLEWIDYQEAVRLHCAGHVAFTAGTLLY
                     TLRGGCNAVTGRRSLIPVHAVLATFGGRYASLRLRPGYVPPLSNPALFRRDFNVCLYC
                     GDSFPVRELSRDHVTPLVQGGRDRWSNVVTACRRCNHLKGGRTPEQAHMPLLAVPFAP
                     THAEYVYLQGRRILADQMEFLRAHFPRSSPLHDRLARAGASVPPVC"
     gene            27656..28780
                     /locus_tag="PK144_00445"
     CDS             27656..28780
                     /locus_tag="PK144_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF64220.1"
                     /translation="MTLRLVVPKETWTGERRVALDPSVAERFQRLGAEVQIERGAGCG
                     SHFGDAQYEKGAKLVDDIVAALAAADVTVRVQPPSLEEVEQLRDGSVLIGFLAPHRNA
                     EVIRRLRDKKITSFAFELLPRISRAQSMDALSSQAAIAGYKVALMAASLASFFFPMLT
                     TAAGTIRPAKVVVVGAGVAGLQAIATCKRLGAMVEAYDIRAAAKEQVESLGAKLIDTG
                     VDASGQGGYARELTKEEIARQAEVLARHIAAASAVITTAAIPGRPAPKIVTKAMVEAM
                     KPGAVIVDLAAETGGNCELTEPGQTIEHNDVTIHGPLNVPSLIPVHASETYAKNVFNF
                     LSPFVKNGELALDWNDEVIAKSCLTHDGQIRHEPTRKLVG"
     gene            28786..29070
                     /locus_tag="PK144_00450"
     CDS             28786..29070
                     /locus_tag="PK144_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF64221.1"
                     /translation="MIEGFAALYIFMLAAFTGYEIIGRVPVILHTPLMSGSNFVHGIV
                     VVGAMVALGHAQTGLEQLLGFIGVVLAAGNAVGGYVVTERMLEMFKSSKE"
     gene            29077..30447
                     /locus_tag="PK144_00455"
     CDS             29077..30447
                     /locus_tag="PK144_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNF64222.1"
                     /translation="MAGLIQIVYFAAAALFILGLKRMSSPKTARSGIVWAGAGMALAT
                     LVTFLYPGLSNYLLILVAIGVGSYAAWVSGKKVAMTDMPQMIAIYNGMGGGAAACIAA
                     VELLKGGSHGAVHALLGVLGALIGAVSFSGSVVAWAKLDGRMKKAIRFPRQKLVNLGV
                     FVLALLFGLIVMGASQPGAFSIIAFFLLALAFGAMMALPIGGADMPVVISLFNAFTGL
                     AVAFEGYVMQNAAMIIAGTVVGAAGSLLTQLMAKAMNRPISNVLFSNFGGGSGATAEE
                     EVKGSMKASEPQDAAVMMAYAAKVIIVPGYGMAVAQAQHKVWELAELLEENSVEVKFA
                     IHPVAGRMPGHMNVLLAEAGVPYDKIFDLDEINNEFASTDVVLVIGANDVVNPVAKSD
                     PNSPIYGMPVLNADQARNVLVIKRGQGAGFSGIENHLFYLDNTRMVYGDAQKVLAELI
                     GFVKTL"
     gene            complement(30554..31012)
                     /gene="dtd"
                     /locus_tag="PK144_00460"
     CDS             complement(30554..31012)
                     /gene="dtd"
                     /locus_tag="PK144_00460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNF64223.1"
                     /translation="MIGLLQRVTRAQVTVAGEPVGAIAAGLLVLVGVERGDGEAQAAR
                     LLERLLGYRVFADADGRMNRSLADTGGGLLLVPQFTLPADTQKGTRPSFTPAAAPADG
                     ERLFRHLLGLAEAAHAPVASGRFGADMQVELVNDGPVTFWLRVPPPGPAD"
     gene            complement(31009..31959)
                     /gene="pip"
                     /locus_tag="PK144_00465"
     CDS             complement(31009..31959)
                     /gene="pip"
                     /locus_tag="PK144_00465"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNF64224.1"
                     /translation="MRELYPDIRPYATRRLPVSPLHTLYVEETGTPGGLPVLFLHGGP
                     GAGCQPVHRRFFDPLRYRIVLFDQRGAGQSTPHAELTDNTTTALIADIELLRKTLGIE
                     RWVVFGGSWGSTLALAYAEAHPERVLGLVLRGIFLCRPQDIRWFYQSGAGRLFPEAWE
                     DYLAPIPETERGDLVSAYYRRLTGDDEVERMRCAKAWSVWEGTTLTLRPSPHTVDHFA
                     DPHMALSLARIECHYFMHDSFLAPDQLVRDVSRLAGIPGYIVHGRYDVICPLDGAWAL
                     HRAWPGSQLDIIADAGHAATEPGIIDALVRATDALADHYR"
     gene            complement(32447..>33024)
                     /gene="hemH"
                     /locus_tag="PK144_00470"
     CDS             complement(32447..>33024)
                     /gene="hemH"
                     /locus_tag="PK144_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNF64225.1"
                     /translation="HDEPAWVDAVAASLDRHAAAHGGLAERVLFSFHGLPKRNLALGD
                     PYHCQCLKSARLIAGRLGLADDRWAVAFQSRFGRAEWLRPYTSETLQQWARTGVRSVD
                     VICPGFAADCLETLEEIAIENRLLFEQAGGERLRYVPALNDAPAHLDALAQLVRRHCS
                     GWPGFAGGPAPHAAAELAASRERALACGADD"
BASE COUNT         4862 a        11543 c        11595 g         4924 t
ORIGIN      
        1 cagggcggcg gcacgggctg agcgcgcgcc ccggccgcgg tgcgcgatcg tgcgacactg
       61 ggcggccgca tcctgtctgt cccgctgccc tcatgtccga tctggccgaa ttcgtcgtca
      121 tcctcaccgc catcgtgctg gccgtgccgc tcgcacgccg ctgccggctc ggggccgtga
      181 tcggctatct gatcgccggc gtcgtcatcg ggccgtccgg actcggcctg atccgcgatc
      241 acgaggcgat cctgcacatc ggcgagctcg gcgtcgtgct gctgctgttc ctgatcggcc
      301 tggagctgaa gccctcgcgg ctgtgggcgc tgcgccggcc ggtgttcggg ctcggcggcg
      361 tgcaggtgct cgcgaccggc gccgtcgcga cgctggcgct gcggctgctc gactacccgc
      421 cggccggtgc gctggtcgcg gggctggcgc tgtcgctgtc ctcgacggcc ttcgtgctgc
      481 agctgctcgc cgagcagggc gaactcggca gccgccacgg ccgcgatgcc ttcgcggtgc
      541 tgctgctgca ggacgtggcg gtgatcccga tgctggcgct gctgccgctg ctcgccaccg
      601 aggcccggcc cggcgacgag ggcgacgggc tgcggcaggc ggcggtcgcg gtcgcggcga
      661 tcacggtcgc ggtgctcgcg agccgttacc tggtgccgcg gctgttccgg ctgatcgccg
      721 ccagccgctc gcacgaggtc gccaccgccg gcgcgctgct ggtggtggcc gccgccgcgc
      781 tcggctgcgc cgagatcggc ctcagcatgt cgctcggcgc cttcctgatc ggcctgctgc
      841 tcgctgattc cgagttccgc cacgagctcg aagccaacat cgagccgttc aagggcgtgc
      901 tgctcgggct gttcttcatc gcggtcggca tgtcggccaa cctcggcctg ctcgccgagg
      961 cgccgctgcg cctgctcggc ctgaccctcg ggctgatggc cctgaaggcg gggctgatgt
     1021 tcggggtcgc gtgcgcctgg ggtcatgccg cggccagcgc cggccgggtc gcggtactgc
     1081 tcgcgcaggg cggcgagttc gcgttcgtgc tgttcgccaa tgccgccggt ctcggcctgc
     1141 tcgatgcggc gcgcgtcgaa gagctgagcg tggtcgtgac gctgtcgatg gcgctgacgc
     1201 cgctgctgct cgccgccctg caggccggcg agcactggct ggccgcgcgc cggccgcggc
     1261 cgccgggtcc gccgctgccg ccgatctcgc cggcgccggt gctgatcgcc ggctacggcc
     1321 gcttcggcca gacgctggcg cgcttcctgc gcgtgcgcca cgtgccgtac acgctgttcg
     1381 acaccgatgt cgcgcgcatc gagctcggcc gccagctcgg cgagaccgtg cactacggcg
     1441 acgcctcgcg cgtcgagctg ctgcgcgccg cgcacgccgc gcacgccgcg gtgctggtga
     1501 tcggcatcga cgatgtcgag acctcgctgc gcgcggcggc gaccgcgcac cggcacttcc
     1561 cgcagctgcg cgtgctgtcg cgcgcctgca acgcccggca cacgctgcgg ctgatggacc
     1621 tcggcgtgca ggcggtggcg agcgacacct acctgtcggc gctggagatg gccgagtgcg
     1681 tcggcgtcgc gctcggcgat gcgccggctg atatccgtgc cgaggtcgcg cgcttccgcg
     1741 agcacgacca gcgcaacctg cgccggctgc atgggctgca ccacgacgag cggcgcatgc
     1801 tggccgcggc gcgcgcggcg gtgctggaga tggaggcggt gttcggcggg cgcgagcgcc
     1861 gtccgccggc cggccgggac gattgaaggt cgcccggacg tgccgccagt gcagccgtcc
     1921 ggacgccgct acgccgccgg gcgccgcggc tgcgataatc gccggcctcg ccgcgcttcc
     1981 caccccggta tgcccgccat gtcccagtcc agttccgctt ccgcgcttcc ccgccgccgc
     2041 gccgtccgcg tgcaggtcgg ctccgtcgcc atcggcggca cggcgccggt cgtcgtgcag
     2101 tcgatgacca acaccgacac tgccgatgcc gtgcgcaccg cgatccaggt cgcgcagctc
     2161 gcccgtgccg gctccgagct ggtgcgcatc accgtcaaca ccgaggaagc cgccgccgcc
     2221 gtgcccgaga tccgcgcgcg gctcgatgac atggggctcg ccgtgccgct ggtcggggat
     2281 ttccacttca acggcgaccg gctgctcgcg aagtacccgg cctgcggcca cgcactcggc
     2341 aagtggcgca tcaaccccgg caacgtcggg cgcggcagca ggcgcgacga gaagttcgcc
     2401 gcgatcgtgc gtctggccgt cgagatggag aaaccggtgc gcatcggcgt caactggggt
     2461 tcgctcgacc cggccgtgct cgcgcggctg atggacgaga accaccgccg gcccgagccc
     2521 tgggacgccg cgaccgtcat gcacgaggcg ctggtcgcct ccgcgctcga aagcgccggg
     2581 gccgccgagg cgctggggct cgcgcacgag cgcatcgtgc tgtcggccaa ggtctcgggc
     2641 gtgcaggacc tgatcgccgt ctaccgcaag ctcgccgcgc gctgcgacta cccgctgcac
     2701 ctcggcctga ccgaggccgg catgggctcg aagggcatcg tcgcctcgac cgccggcctc
     2761 gccgtgctgc tgcaggaagg catcggcgac acgatccgca tctcgctgac gccggagccc
     2821 ggcgcggcgc gcgagcagga ggtcatcgtc gcgcaggaga tcctgcagtg catgggcctg
     2881 cgctcgttca cgccgatggt gatctcctgc cccggctgcg ggcgcacgac ctcggactac
     2941 ttccagcgcc tcgcgcagca gatccagtcc tacctgcgcg cgcagatgcc gctctggcgc
     3001 gccgagcacc cgggcgtcga ggccatgacg gtcgcggtga tgggctgcgt cgtcaacggc
     3061 cccggcgaaa gcaagatggc caacatcggc atctcgctgc ccggcgccga cgaggatccg
     3121 gtcgcgccgg tctacgtcga cggaaaaaag accgtgaccc taaagggcga cacgattgcg
     3181 gccgatttcc aggccatcgt cgatgactac gtgaagtcgc gtttcacccg gacgtaggcc
     3241 ggacacaggc ccggggcagc gcgcctagac tggattcccc atgcccgcac agacgggccg
     3301 gtacaccaga ggagagcgtc gggccatgaa gattcaggat ctcacggtgc tgctggtcga
     3361 gccctcggcc acccaggcaa agatcgtgcg ccagcagctg caggccctcg gtgtcacgcg
     3421 catcgagacg gtcacgagcg gcgccgcggc gctgccggtc gtgcaggcgc gcgacctgca
     3481 tctggtcgtc agccgcctgt acctgcccga catgtccgga gccgatctct ccaagaccat
     3541 caacgccctc gaccggccgt tccgcccggc cttcatgctg atctccagcg agaccaacga
     3601 ggcctatctc gaaccgctgc gccaggccgg cgccgcggcg atcgtgcgca agcccttcga
     3661 cgcgagcttc atgcaggagg cgctgcacgc ggtgctcgat cacctcgaac cgggcacgct
     3721 gcacctcggc gagcgcgaca tcgaggagat ccgcgtgctg ctcgccgacg attcgacggc
     3781 cgtgcgccgc ttcctcgtgc agctgctgcg cggcctcggc atcgatcgca tcgacgaggc
     3841 cgacgacggc gcgacggcga tgccgttcat cgaccagcac tactacgacc tgctgatcac
     3901 cgacctgcac atgccgctgc tgaacggcat cgagctgacg cgctacgtgc gctgcgagag
     3961 ccagcagcgc tcgctgccga tcgtgatgat caccagcgag gccgacggcg ggcggctgga
     4021 catggcgcgt gccgccgggg tctcggcctt cctgcacaag cccttcgagg ccaaggcgct
     4081 gaaggcgcac atcgagcggc tgctggccgg ctgactgtga agatgcgtgg ccgcagggag
     4141 tacctattcc gatcgtaggg tttgccgggc gatggttcgg ggcacaatcg ccggcatgca
     4201 gatgctggcc gggcatgaac ccggcggctc accggcgcgg tgcatgaagc tgcgtcttac
     4261 aggactccgg gagaaccctg ccgatgaacg accgccgccc gccggatgac gatcccctgc
     4321 tgcaacgcct gggcgaacag ctcgaacgcc gcggcgtgca tgccgggacg gagacggcgc
     4381 gggcgctgct ggagcgcgcc tcggcggaac tgcggcgcgg cgccgatctc gaccagctcg
     4441 ccgcacgcct ggccgagcag gcctgtgcgg cggcgcggcg cgtcagcagc agcgccgccc
     4501 agccggtcga gcggctgatc gcggtcgggg cctcgaccgg cggtccggat gcgatccgca
     4561 ccttcctgcg cggcctgaac cggcgctcgc cgggcgtcgt gatcgcgcag cacatgccgg
     4621 ccggcttcac gcgcctgttc gccgagcgcc tgacgcagac caccggcctc gaagtgatcg
     4681 aggccgccgg caacgagtcg atcctgcccg gtcacgtcta catcgcgccg ggtcacgcgc
     4741 acctgaccgt gcgccgcgcc ggcagccact tcctgacgcg gctgtcggac gccccgccgg
     4801 tcaacgacgt gcggccctcg gtcgacgtgc tgttcgaatc gctcgccggc tgcgccggcg
     4861 agcacgtcac cggcgtgctg ctgaccggca tgggccgcga cggtgcgcag gggctgctgg
     4921 cgatgcgcca ggccggcgcg cgcaccttcg cgcagaacga ggccacctgc accgtctacg
     4981 gcatgccgcg cgcggccgtg cgcatcggtg ccgccgaaac cgtgctgccg atcgagcggc
     5041 tggctgcggc cgtgctcggt gagggggccg ggggcatcag cccttttgaa gccagtcccc
     5101 gatcagccgt cgcgacaggc tcatttcgtg cggcagtccc ggcattcggc tgcgtgtgaa
     5161 ccacgcggcc tcgatgatct cgacgccgtc gatcgcgatc tcgccaccgc tccattccgc
     5221 ggtgaagccg atcatcagcg aatgcggaaa cggccacggc tggctcgcga agtagcgcag
     5281 tccgctgatt tccagcccga cctcctcgcg cacctcgcgc gtcacggtct gttcgagcgc
     5341 ctcgccggcc tcgacgaagc cggcgaggat cgaccagacg ccgggcgcga agcgcggcga
     5401 gcgcgcaagc aggatttcct caccgcgcgt cacgcgcacc atcaccgccg gcgacacgcg
     5461 cggataactc atgtggccgc aggccggaca ggcgcgggcg cgctcgtcgg tgcgtgattc
     5521 ggtcggcgtg ccgcagaccc cgcagaagcg gtgcgtggcg tcccaggtca gcagctgcgc
     5581 ggcctgcgcg gcgatgccga gcagctccgg atcgagctgg ccccacagcg cgcgcaggct
     5641 gacgaaggct gcgccgggca gcggcccggc ttcgggcggc acggcgagcg cgcggcaggg
     5701 cacgccgtcg aagtggccga ggccgagcac gctgccgggc agcggcgcgt cgcggccgaa
     5761 cggcaggctg gcgccggcgg cggcgcgcag caccagcagg cggccatcct gcagcgcgaa
     5821 ccaccagccc ggtgccgccg ggtcggcggc gggcacggcg gcgacgtagc gatcgggcag
     5881 ttgcagcatc gggcggctcc tcggcgacgg tgacgcggcg catcatcgca cggccgcggc
     5941 cgctggccgg ggctcagagc tcggtcagcg ccacgctggc ggtgtcctcc agtccgagca
     6001 gccggcgcac gcgccagatc gccgtcgaca ccttgccctc gatcagcagc cggcccggga
     6061 cttcctcgat gccggcacgt gccagcacct tcgccggctg gccgcgcacg ccggcgagca
     6121 cgacgcagat gccgtcggca tgcaggcggc ggatcgccga atcgagcgcg acgacgccgg
     6181 agatgtccat gaccggcacc gattccatgt cgaggatgac gacctcgcag tggtcatcga
     6241 tgcggtgcag cgcgctcatg gccttctcgg cggcgccgaa gaacagcggg ccggcgacct
     6301 cgtacagccg cgtgtgcgcc ggcagggccc cggcatgggc gtgggtttcc tcgccctgca
     6361 ggcgcatgcc ggcgaactcg gccatgcggt tcatgaacag cagcgccgcg agcacgacgc
     6421 cgacgccgac cgcgaccacc atgtcgaaca ccacggtcag cccgaagcag accagcagca
     6481 cgatgatgtc gctcttcggc gccacccgcg tgatgtgcag gaagtgcttc atctcgctca
     6541 tgttccacgc caccagcagc agcagcgccg ccagcgccga catcggcagg taaccgagca
     6601 gcggcgccag cgcgatcagc accagcagga tgaacagcgc gtggatcgcc gcggccagcg
     6661 gcgagcgcgc gccggcgcgg atgttggtcg cggtgcgcgc cagggcgccg gtcgcggcga
     6721 tgccgccgaa gaacggtgcg atcaggttgc cgaggccctg accgatcagc tcggcatccg
     6781 ggtcgtggcg ggtgccggcc atgccgtcgg cgaccaccgc gcagagcagc gattcgatcg
     6841 ccccgagcag cgcgatcgcc atcgccggcc cgaccagcga cttgaccgtc gccagcgtca
     6901 gtccgagcgg cgccccgtcc gcgcccggca ggttccacgg cagcacgaac tgcggcagca
     6961 gcgccggaat gccgtgcagg gtttcgccat cgagcgtgta cgagaagcgc gaggcgatgg
     7021 tcgcgacctc gacgtggaac agccgttcga gtccgtacgc gagcagcgtg ccgacggcga
     7081 gcgcgacgag gtgctgcggg atcggtgtgc gcagcttcgg ccacaggatc agcaccgcga
     7141 gcgtcaccgc gccgaccagc aggtcggccg gattgaggcc gggcagcgcc agccccacct
     7201 gatgcatgcg ctcgaagaag ctctcgggcg cgtgcgcgaa gctcaggccg aagaaatcct
     7261 tgaactgcag cgacgcgatc acgacgccga tgccggccgt gaagccggtc gtgaccgggt
     7321 aggggatgaa ctggatcagc ttgcccatgc gcccgatgcc catgccgagc agcatcacgc
     7381 cggccatcag gctcgcgagc acgagcccgc ccatgccgta ctgctcggcg atcggatgca
     7441 ggatgacgat gaaggccgcg gtcgggccgg agacgctgaa gcgggcgccg ccggtcagcg
     7501 cgatcagcag gccggcgatc accgccgtgt acagaccgta ctgcggcggc acgccgctgc
     7561 cgatcgccag cgccatcgcc agcggtacgg cgacgatgcc gaccgtgatg ccggccagca
     7621 ggtcggcgcg caggtcgctc acgccgtagc cggcacgcag cgtctcgcgc agcgccacgg
     7681 caaagcgcgg ccctcccgag tgttcttccg tcggtttcat gttctgcctt gtcggtgtgc
     7741 cgctcgcccg cggccgatct ggtgcagccg cccacccccg gggcggcgat gaccgggcga
     7801 tgctagcacg caccatgccc cggccgacgc gggaaaggac cggctgatcc acggtttggc
     7861 ggccgttttc cggttctgcc gcgcttctgg cgcggtgatt gctttggcgg caggcaagcg
     7921 gccactgtca cgaacccttg ccgaaaccgg caatgctgcg ctgctgatga cgcagggcgt
     7981 ggctagactc cagaccggct cgccgcccga tgctgcgacg agttgcacac gaagcagcgc
     8041 gcacgattgg tccttccctg tcaatcgatc ttgacgcgga ccgaccgcgg cagcgactcc
     8101 catcaccccc ggaggtaatt catgcgcaag tccagccaca gtgccgccgt cgtcacggcc
     8161 gccctgctcg cctttgccgg caccgcccac gccggtgcca cgctcgatgc ggtcaagaag
     8221 aaaggcttcg tgcagtgcgg cgtcagcgac ggcctgccgg gcttctcgta taccgacgac
     8281 aagggcaacg tcagcggtct cgatgccgac ctctgccgcg cggtggcggc ggcggtgttc
     8341 ggcgatgcga ccaaggtcaa gttcacgccg ctgacggcca aggagcgctt cacggcgctg
     8401 cagtcgggcg agatcgacat cctgtcgcgc aacacgacct ggacttcctc gcgcgacacc
     8461 gcgctcggcc tgaacttcac cggcgtcacg tattacgacg gccagggctt cctcgtgaac
     8521 aagaagctcg gcgtcaagag cgcgaaggag ctcgacggcg cctcgatctg catccaggcc
     8581 ggaacgacga cggagctgaa cctcgccgac tacttccgcg ccaacaacat gaagttcacg
     8641 ccgatcagct acgacaagtc cgacgagagc gcgaaggcga tggaagccgg ccgctgcgac
     8701 ggcctgacct ccgaccagtc gcagctttac gcgcagcgca tcaagctcgc cgcgcccgat
     8761 gactacatcg tgctgcccga ggtcatctcc aaggaaccgc tcggcccggt cgtgcgccac
     8821 ggcgacgacg agtggttcga catcgtcaag tggacgctgt acgtgcaggt cgccgccgag
     8881 gaatacggcc tgacctccaa gaacgtcgcc gaggccgcca agacctcgac caaccccgac
     8941 gtgaagcgcc tgctcggcgc cgagggcgag aagggcaagg acttcgggct ggccaacgac
     9001 tgggtggtca acgtcgtcag gcaggtcggc aactacggcg agatgttcaa ccgcaacgtc
     9061 ggtgacggca gcccgctgaa gatcaagcgc ggcctgaacg ccctgtggaa ccagggcggc
     9121 ctgcagtacg caccgccctt ccgctgagcc gcgccggccg catgacctca ccggcggcgc
     9181 cggactcgcg ccgccgcacc gacccgagcc cgcggcggcg ccggatgcgc cggaccccgg
     9241 gccgtgcgcg gtgacgcgat cccccgatcg cgccgccgtc cggagcgcct cccatgacta
     9301 cgcccctcgc aggcgcagca cctgcccggc cgccgttctg gaacgatccc gcggtccggg
     9361 ccctggtctt ccaggcgctc gcgctcatcg ccgtcatcgc cctcggctgg tatctggtcc
     9421 acaacacgca gcagaacctc gcccagcgcg gcatcgcctc gggcttcggc ttcctgaaca
     9481 acgctgccgg cttcggcatc gtccagcacc tgatcgacta ctcgccggcc gacagctacg
     9541 gccgcgtgtt ctggatcgga ctgctgaaca cgctgctggt ctcggtgctc ggcatcgtgt
     9601 tcgcgaccgt gctcggcttc gtggtcggca tcgcgcggct gtcgcgcaac tggctggtcg
     9661 cgaagatcgc gatggtctac gtcgagatct tccgcaacat cccgctgctg ctgcagatct
     9721 tcttctggta cttcgcggtg ctgcgcacgg tgccgaaccc gcgcaactcg ctggcgttcg
     9781 gggatgcctt cttcctgaac atccgcggcc tgtacgtgcc gcgcccggtg ttcggtgacg
     9841 atttcgtctg ggtgctcggc gcgctggcgc tggccgtcgt gctggccgtg ctgctggggc
     9901 gctgggcgcg gcagcgccag gcaacgaccg gccagccctt cccggtgttc ctggtgtcgc
     9961 tcgcgatcct cgtcgtgctg cccggcgtga cgtacttcgc agccggccag ccgctcacct
    10021 gggacatccc caagctcgcc ggcttcaact tccgcggcgg ctgggtggtg attccggaac
    10081 tcgcggcgct gctggtggcg ctgacgctgt atacggcgac cttcatcgcc gagaccgtgc
    10141 gcgccggcat cctgtcggtc agccacggcc agaccgaagc cgccggcgcg ctcgggctgc
    10201 cgaacggcct gacgctgcgc ctgatcgtga tcccgcaggc gatgcgcgtg atcatcccgc
    10261 cgctgaccag ccagtacctg aaccttgcca agaactcgac gctggccgcg gcgatcggct
    10321 accccgacct ggtcgcgacc ttcgccggca cggtgctgaa ccagaccggt caggcgatcg
    10381 agaccatcgc gatcacgatg tcggtgtatc tggcgctgtc gctgggcatt tcgatgctga
    10441 tgaactggta caacaagcgc aaggcgctgg tggagcgttg atcatgaccg agccgaatcc
    10501 acccgcggcc accttcacgc cgcatcccga cctgccgccg ccggccgcca ccaccggcgt
    10561 gatcggctgg ctgcgcgcca acctgttctc gtcgccgctg aacgcgctga tgacgctgct
    10621 ggtcgcggcg gcgctgctgc gctggatccc gccgatcctc gactggctga tcttcagcgc
    10681 cgactggagc ggcagcagcc gtcaggactg cacgggcgag ggcgcctgct gggtgttcat
    10741 caagcagcgc ctcggccagt tcatctacgg cttctacccc gaggaccagc gctggcgcgt
    10801 gaacctggtc tgggtcatcg ccgtggtcgc ctcgtggccg ctgttcctga agcgcaaccg
    10861 cctgcaggtg ccggccgggc tgttcatgct gttcgtgttc ccggtgatcg cctacggcct
    10921 gtataccggt ccgctgttcg gcctgtcgca cgtcgagacc tcgcagtggg gcggcctgat
    10981 gctgacgctg accatcgccg tggtcggcat cgccggctcg ctgccgggcg gcatcctgct
    11041 cgcgctcgga cggcgctcga agatgccggt gatccgcacg ctgtgcgtga ccatcatcga
    11101 gttctggcgc ggcgtgccgc tgatcaccgt gctgttcatg tcctcggtga tgctgccgct
    11161 gttcctgccc gagggcatga gcttcgacaa gctgctgcgc gcgctgatcg gcgtcgtgct
    11221 gttcgagtcc gcctacatcg ccgaggtcgt gcgcggcggc ctgcaggcga tccccaaggg
    11281 ccagtacgag gccgccgatg cgctcgggct caactatccg aaggcgatgg gcctgatcat
    11341 cctgccgcag gcgctgaagc tggtgatccc cggcatcgtc aacacgatga tcgcgctgtt
    11401 caaggacacc tcgctggtca tcatcatcgg gctctacgac ctgctcaaca gcatccagca
    11461 ggccgcggtc gacccgaact ggctcggctt cgcgaccgaa ggctacgtgt tcgccgcgct
    11521 gctgtactgg gtcttctgct tcagcatgtc gcgctacagc cagcatctgg agcgtcgcct
    11581 gcacaccggc cacaagcgct gagcgcgcct ccacttcgag gaaattcccg atgagcgata
    11641 ccgcctccag cccgaagctc gtgatcggcg acaaggtcat gatcgagatg aagggcgtgc
    11701 acaagtggta cggccagttc cacgtgctga agaacatcaa cctgaacgtg cgccagggcg
    11761 agcgcatcgt gctctgcggc ccctccggtt ccggcaagtc gacgacgatc cgctgcatca
    11821 accggctgga ggagcaccag caggggcaga tcatcgtcga tggcacggag ctgacctccg
    11881 acgtcaagca catcgaggcc atccgccgcg aggtcggcat ggtgttccag cacttcaacc
    11941 tgttcccgca cctgacggtg ctggagaact gcgtgctggc gccgatctgg gtgcgcaaga
    12001 tgccgcggcg cgaggccgag gaagtcgcga tgcagtacct caggcgcgtc aagattcccg
    12061 atcaggcgaa gaaatacccg ggccagctct ccggcggcca gcagcagcgc gtcgccatcg
    12121 cccgctcgct gtgcatgaag ccgaagatca tgctgttcga cgagccgacc tcggccctcg
    12181 acccggagat ggtcaaggaa gtgctcgaca ccatggtcag cctcgccgag gacggcatga
    12241 ccatgctgtg cgtgacccac gagatgggct tcgccaagca ggtctcgaac cgcgtgatct
    12301 tcatggaccg cggcgagatc atcgagcaga acgacccgca cgagttcttc aacaacccgc
    12361 agaacgagcg cacgcggctg ttcctgagcc agatcctgca tcactgagtc agggcagcag
    12421 agcgtcatcc ggggccggcc atgccggccc cgtgcatttc cggggaacgg gaagatccgg
    12481 ctcagcggcg cgggcccgcg ccccgccccc cggccgtcac cgtcagcacc gcccaggccg
    12541 cgccgaccac ggccgcggtg cgcagcagcg ggctgaagcg ggtatccggc agcggcccca
    12601 gcgcctgcgc gccggcgccc gccggggctc gcaccgcagg cccggccggg cggttccgct
    12661 gcggcagttc gcgcccgccc ggcgccgggg ccgggcggct gcggggctcg ccgcgacgcg
    12721 agccgtgagc gtgcgcgtac acccaggtga attcggcgcc gaggaagaag atctgcgccg
    12781 agtagtacac ccagaccagc agcacgacga aggacccggc cgcgccgaag gccgaggccg
    12841 tgctgctctt gccgagatac aggccgatca gcagcttgcc gatcgagaac agcagcgccg
    12901 tgatcgccgc cccggtccac acgtcgcgcc aggcgatgcg cgcgcgcggc agcagcttgt
    12961 agatcagcgc gaacgccgcc gtgatcagca cgaaaccggc gatgaagttc gccgcctgcg
    13021 cgagcagctc ccgttcgccg aaccacggcg accaccattc gccgagcgcc gcgatcaccg
    13081 cgctcagcac cagcgacacc agcagcagga aggcgatgcc gagcaccatg ccgaaggcga
    13141 gcagccgcgt gcgcagcagt gcccacagcc cttccgggcg ggtggcggcc ggggcctgcc
    13201 agatgcggtc gagcgaggtc tgcagctcgc cgaacacggt cgtggcgccg atcaccagcg
    13261 tcacgacgct gatgacggtc gcgaccatgc cctgctgcgg atcgctcgcg cttttcagca
    13321 gccactcgat cgccagtgcc ccgtccttgc cgagcaggtc gcgcagctgc gcgaggatgt
    13381 gaccctgcgc ggcatcgtgg ccgaacacgg cgccggcgat cgcgatgacg atgatcagca
    13441 gcggcgcgat cgagaaggtc gtgtagtacg caagcgccgc gcccatgctc gccgcgtagt
    13501 catcgctcca ggccgacacc gcctgccggc ccatctgcca gatctgtccg ggactcacga
    13561 ttgcgctctc ctcggggtcg cgacagcgcg cagggctgcc gatgactggg agggcggcgc
    13621 ggggcggagg ttgcagccgg ccggttcatg ccgagccttc cgccgcaaca ctccgcctcg
    13681 cagttcctcg ccccgagtgg acgagggctt gcagggatgg gcggcgggat catgcacttg
    13741 cgggtgatcg gcctgcggca tcggccagcg tgaatcctgc cctgaccgac cgcgacgcac
    13801 atgcgccggc ccgctgcaga gtcccgatgt cgccccagat tgagtcgacg ccgcctcaaa
    13861 atgagtcggc gtcgacactt caagtatcgg cgtcgatggt ttgagtgccg gcgacgctgc
    13921 cctgaccctc cggaacagcc ttccggatgc cggcgccgct gtttgaagtg ccgattccgg
    13981 agttcaaaac cccggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntccg
    14101 gatgccggcg ccgctgtttg aagtgccgat tccggagttc aaaaccccgg catcggggtg
    14161 cggaacaccg tcatcgacac ttcaagtctc gacgccgact cattctgggg cggcgttgac
    14221 tcaaaaagag tcggcgtcga ttgttgaagt gtcggcgccg actgttcagg agtcggcgtc
    14281 gactgttcag gagtcgacgc cgatagttca ggggtcggca tcgactgttc ggcacgcgca
    14341 ggctgggttg acgaaggaag cccggcaacc atcacgcgtc atggcttccg atgccgggtt
    14401 tcgttcctca acccagcctg cgagctgtct tgtctttcag gcatcggcct ctgtcgtcgc
    14461 ccgcggccgc agcgcccacg ccatcacgaa cccgaagcaa agctcgcgca cgaacgccac
    14521 cttggcggtg ccgctcgcga gccaggccag cgcgtcgccg ccccgcagtt ccggcagcag
    14581 gttcaaagcg aggaacaggc cgaggccggc gagcaggccg ttgagggtga tgaaggcgag
    14641 gcggcgggcg gggtgcatcg ggcgctggtt cctgttggcg gacgatggaa acgcgacagc
    14701 cgcccgcggc ggggccgggg cggctgtctg agagaggggg acggcatcct gcgtcatggc
    14761 ggcattcctt gcggcggtgc tccttacgat atcggcatcg gtgccgattg caaagatgcg
    14821 tcacgcagcc tgtgttcagg cgtggcgcac gctcgcgagc agcgcgccgg ctccggcgaa
    14881 catgccgccg aacacgcggt tcagcaggcg catgcgccgt gcgttctgca cgtgccggcg
    14941 cagcacgccg gcgagcgtcg catagccgca catgacgatc acgtcgacga ccaccagcgt
    15001 gccaccgagc agcgcgatct gcatgccctg cgggcgcgcc gggtcgagga actgcgggaa
    15061 cagcgcgacg aggaacacga tcgccttcgg gttggtgacg ttgacgatca ccgcctgcca
    15121 gaacagccgc gccggaccgg gcagtgccac ggacttctcg ctcacgtcga agtgcgtcgg
    15181 ggcgtgccac ttctgccagc cgagccagag gagataggcg acgccggccc acttgatcgc
    15241 cgtgaacgcc gtcgccgagg ccgcgatcag cgcgccgagg ccgacgccga ccagcagcgt
    15301 gttgacgatc agcccgccct gcaggccgag gatgccgatg aaggtgcgcc ggcggccgta
    15361 gctgagcgcg ttgctcatgg tgttcacggc gcccgcgccc ggtgaaatgc tgatcagcac
    15421 acaggcggcc agccaggcca gccagacgtg caggtccatg atgtcgcccc ctcgtgaaaa
    15481 cgcgccggat cggcaggccg gcaagtctgc gccgcgccgc gcagcggcca tagcggcgca
    15541 tggctcgcat gcgcggccgt caggagcggg gcagggggcg gcgggcgggc cggctcaggg
    15601 cagcgcgcgc accatcagca gcacgtcgcc ggcgtaggcg agcagcgggt gcggcaggat
    15661 cggccggcgc tcgaccaccg tgaagccctc gcgccggtac aggcgcgtgg ccgggttgtg
    15721 ctcgcaggcg atcaggctga cgcgatcgaa gccggcggcg cgtgcctgcg agcaggcctg
    15781 caccagcagc cggtggccga ggccgtagtt gcgccacggc gagaagatgg tcatgccgac
    15841 cagcagccag gtgtccggca cctgcatgcg caccggcgcg cagagatcgc tgccatcggc
    15901 cccgagcacc gccgcgaccg gcggcacctg cgcggcggcg cagccgagca gcacgccggc
    15961 gacggcgccg tcctgggttt cgagcaccag cgcattgcgc caggaatgcg ggccgtcatc
    16021 ggcggcgcag cagcgggcgc cggtggcgtg caggtcgccg tcgccggcga gggccgacca
    16081 ggcgagcggc gcgtagccgc ccgaggcctc ggcgatggca ccggcgacga tcgcggcttc
    16141 ctcgacgcgg gccgggcgca ggctgaagtt cagcgcggac atggcggcgg ctcccggcgg
    16201 gtcattgccg cgccgtgcgc aggttctcgg gccagggccg ggcgctgtgg ctggcccgga
    16261 acgggttgat gtcgagtccg ccgcggcgcg tgtagcgcgc gtagacggcg agcgattccg
    16321 gctggcagcg ttcgtggatg tcgcgccaga tgcgctcgac gcagtgctcg tggaattcgt
    16381 ggtggttgcg gaacgtgatc aggtacttca gcagcccggc ccggtcgatc ggctggccgg
    16441 tgtagcggat ctgcacgctc gcccagtccg gctggccggt gacgaggcag ttggtcttca
    16501 gcaggtgcga gaccagggtt tcctcgaccg gcttgcgcgg gacgccggtg gccagcatca
    16561 gcagtgccgg atcgggcggg ccgtaggact cggggctcac gtcgagctcg tcgatgcact
    16621 ggccggcgag ttcctcgctg cgctgcgcgg cgaaggcttc ggggccggtc aggtgtacgc
    16681 cgatcagcgt gccgaaggcc gcgccgagat cggccgacag gcgccgcagc agatcggtgc
    16741 gatcgcgcag ccgggtctgg gtgaaggagt tcaggtacag cttcagcgac ttggattcga
    16801 ccaggttcgg cgattcggcc ggcacggcga aactcgccat cgccacctgc ggcttgccgg
    16861 ccggcgtcag ccatgacagc tcgtaggcgt tccacagatc gacgccgacg aatggcagcg
    16921 tgttatcgga gaagccgagt tgcgcgcgct gcccggcgcg cggcaccgtg cacagcaggg
    16981 cgggatcgta atgttcggag ccggcgacga agcggccgag cggcaggtgt gacagatcac
    17041 tcatgcggga aatccggggg aggcggcggg gggcgcggcg cgtccgggat tccggcggag
    17101 cgccggcctc ccggcggcac cccgttgcgc aggtgatcgg gccgcagcgt aacaggctgc
    17161 ccgcaagctg tgccacgccg tcgcccccga ggaggtagcg gcgcatcagg ccgacggggc
    17221 agggcgcgtg ccgccggccg ggcggggctg cagctgcagc gtcagcgtgc cggcggcatc
    17281 ggtgagctgg cagcggccgt gctgcagccg gcagtcgaag cgcatcgtgc gcgccatccg
    17341 ggcggcgagg gcggcggttt cggctgcggc cagcagccgc acctccagcc ccggcagggc
    17401 ctgcacccgc gccggcagcc gctgccacca ggcttcggcc gcgcgcccgc caaaggccca
    17461 gaccgacacc cgcggtgccc ggcccgccgc ctgcagcagc cggcgggcgc cgggcaggcc
    17521 gacctcgacc cacagcgccg gcgagccgtc ggcggccgtc tgcagcagcg ccggttcgcc
    17581 ggccacacag agatcgcgcc cgaagttaag agctgtatcg gcgtggccga tgaaggccag
    17641 caggcggatc atcagtcgct cggcggtttc ggacggatgg cgcgcgaggc gcacggcata
    17701 gacaccgtga tgcccgcggt cgaggtcgtc gatgcgcaga tcggcgcgca gcaggccgct
    17761 tcgctcgccc atgttcgtat cgctgcctcc gggggctgcc gcctgcgccg ggcaacaggc
    17821 atagtgcgcg gtccgcggcg cgtcagggat gcgatgtttt gctcaggagt ggccgggcgg
    17881 cctgcgccgg gacccccttc atgaccgcgg caccagccgg gaggccgggc cgccctcgac
    17941 cggatggtgc gcgggatcat gctgcacgct tcgtcattgc tgccgccgct gacgccggcc
    18001 ctgcgggggc gactggcctt gctggcggcc ggctacggcg tgttcgccgt cgtcgccatc
    18061 ctgctgtcgc gccagcccgg cagcctcgcg accgtctggt tcgcgaatgc ctgggcgctg
    18121 gtgttctggc tgcgcagccg tcaggtgctg ccgcggcggc tgcaggcact cgccgtgctg
    18181 gtggcggcct ggatcgccaa ctacgtctgc aacacgccgg cgacgctgtc gcttgccttc
    18241 gccggcgtca atctcgccga gatcgtgttc ggcggcctgc tgatcgagcg tcatgcccgc
    18301 gagcggcccg agagcgccgg cgcgatgctg cgcctgctcg tcttcggcgt gttcgtgccg
    18361 ccgctgctcg gcgcgacgct cggggcgctg ctgctgcaga tcgccggcat cgccgaagcc
    18421 gtgccggtgt ggccgctgtg gtactgcggg tcggtgatcg gcgcgctggc gctgctgccg
    18481 ctcggcgtga cggcgacgcg ggcctggggc gatgcccgcg ggcggctcga aaccctggcc
    18541 ggagtcctgg gggcggcgct ggtcgccggg ctggtgctgc cgaatctggc gtttccgtat
    18601 gtctacgtga cgctgccgct ggtgctcgcg gccatgcgcc tgccggtggc cggcacggcg
    18661 ctggctgtct gcgtcaccac ggcggtctgg gcgctgctgc tcgccttcga gcgcattccg
    18721 gcgtcggagg gggggccggt ctggacgctg ctgacgccga tgatcgtgac gctgctgccg
    18781 gcgctgctgc tggccgccgc ggtcgccgag acccggatgc agacccggcg cctggccaat
    18841 cgcgaacgct tcgtgcgcct cgtcaccgat gcgctgccgg gcatggtcgg ctactgggac
    18901 tcgggtctgc gctgccgcta cgcgaaccgt gcctaccgcg aatggttcgg ccgcgagccg
    18961 gaatccctgt acggcgagac gatgcagtcg ctgctcgggc cggcgctgtt cgcgaagaac
    19021 gaaccctacg tgcgtgcggt gctgcgcggc gagccgcagc gcttcgagcg cacgctggtc
    19081 aaggccgatg gcagcaccgg ccacacctgg gcgcactaca ttcctgactg tccgggccct
    19141 gaccgtccgg gctccggccg tccggacggt gtgaacgggc cggtcgccgg cttcttcgtg
    19201 ctggtcaccg atgtcaccga gctgaaggcg gccgagcttg cgctcgccag gctcaacgcc
    19261 gaactggccg agcgcagcgt gcaggccgag gccgccaacc gcgccaagag cgagttcatc
    19321 gccaacgtca gccacgagat ccgcacgccg atgaacggca tcctcggcct gctgcaggtg
    19381 ctggaggaca ccgcgctcga cagccgccag cgccgctacc tgcgcacgat ccgccagtcg
    19441 gcgcgcgcgc tgctcgacat cctgaacaac atcctcgatc actcgcgcat cgaggccggc
    19501 cgcctcgacc tgcatcgcga ggacttcgac ctgcccgaac tgctcgaagg cgtcgtcgac
    19561 ctgttcgcac cggctgcgca ccagaagcgg ctggagctgc tgctcgatgc cccggccggg
    19621 ctgccggcgc gcctcacggg cgatgcgctg cgcctgcatc aggtgctggt gaacctgctc
    19681 ggcaacgcgc tgaagttcac cgagcgcggc gagatatgcc tcggcgtcga gcgcctgccc
    19741 ggtgccgctc ccggctgcgc gcggctgcgc ctgcaggtgc gcgacaccgg catcggcatg
    19801 aacgaggccc agctgcagcg gctgttcacg ccctacgtgc aggccgacgg ctcgaccgcg
    19861 cgccgctacg gcggcaccgg tctcggcctc gtcatcagcc agcagctcgt gcgcctgatg
    19921 ggcggcgaga tcacggtgag cagcgcgccg ggtgccggtt cgaccttcgg tttcgaactc
    19981 gaactgccgc tcgccggccc gccggtgcgc agcctcgcac cggtgcccgg gcggctcgcc
    20041 ggcgtgcgcg tgctgctcgc cgatgccggc gccaccgccc gcggcatcgt cggccgggcg
    20101 ctggagcact ggggctgcga ctgcgtcgag gcccatgacg ccgatgcgct gctcgacacc
    20161 gcgctctcgg ccgccgggtc cggccggccg ttccgcttct gcctgatcga tttccggctg
    20221 ccgccgcagg gcgcgctgaa ggcgcagcag cggctgctgg ccgagcgcaa cgccggccgg
    20281 ctgccggcct cgacgacgat cctgctgctg accgagggcg agcggctcga actcgacggc
    20341 gagccgctcg ccgccgggct gccggtgctg atgaagccgg tcaccgcctc gcgcctgcac
    20401 gacgtgctga tcgaacacct ggaccatgaa ctcgggatcg acaacaccag cgtgccggct
    20461 ccgggcgagg actggcaccg gctcggtgcg gtgctgcgcg gcgcgcaggt gctcgtggtc
    20521 gaggacaacg ccgtcaaccg catggtcgcc gaggaactgc ttgaacggct cggcatccgc
    20581 gtcaccgctg ccgccgacgg cggcagcggc gtcgagctgg cgctggccgg gcgctatgac
    20641 gcggtgctga tggacctgca catgccgggc atggacggct tcgaggccac agcgcgcatc
    20701 cgcgccgatg gccggcacga tgcgctgccg atcatcgcga tgaccgccgc cgtgttcgac
    20761 gaggaccgcc gcgccaccgc cgccgccggc atgaacgatc acctcgccaa gcctgtcgag
    20821 cgcgacgagc tgatccgcgt gctgcgccgc tggatcgcgc cgcgcatcag cacggccccc
    20881 gtgccgttcc cccccgccgc caccgaggtc gatgccatgt ccggagcccg tcctgtcgtg
    20941 cccggtgccg ccgtgcctgc cgatctgccc gccgaagccg gcgatgaggc gctgccggtg
    21001 ctgcccggct ttgatctgga cagcgcctgc ctgcgcctcg gcggcgatcg cgcgctgctg
    21061 cgccggctga tgcagacctt cgcgagcggt tacgccgatg ccggggtccg gctgcgcgcc
    21121 gcgttcgcgc agccggcgcg cagcgaactc gtgcgcctgc tgcacacgct caagggtgcc
    21181 agcggcagta tcggcgccac cggcctgcag gccgaatcgg cacggctgga aaccctgctg
    21241 cgcggcggcg gcgagcccgg ggacatcgag gcgctgagca cgctgctcga tgcgacgctg
    21301 gcgacgctgg cccggtatct cgatgagacc gcgacgccgg ctgccgctgc ggccgtgccg
    21361 gttccgccac ctgcctccgc aaccctgtcg gcctcgctgc gcgaactgca gggcttgctc
    21421 gaatcacacc ggctggtgcc ggatgcgctg gtcgcgcgcc tgcgcagcga ctgcggggga
    21481 cacgaagttg caatcaagct gctcacggcc gtggataatt ttgattatga acaggcctcc
    21541 agatacctgg agttgctgat aacggagtac gagcatgacg gcctggggac ctccggacca
    21601 tcacgacctt catgagcagc cgccacgcgt gctgctcgtc gatgacgcac cgatcaacct
    21661 ccagttgctg gcctccgcgc tcggccgcgg gtatgagctc cacgtcgccc gcgacggcgc
    21721 ggcggcgctc gactgcatcc gtcgcgaacc cgatctcgat ctggtcctgc tcgacattca
    21781 catgcccggg ctcgacggct tcgaggtgct gacctggctg cgcgaggaat tccccgaacg
    21841 ccggctgccg gtgatcttcg tcaccgcggc cggagacgaa caggcgcaga ccgacggctt
    21901 cgcgctcggc gccgtcgact acatcgtccg cccgttctcg ctgccggtcg tgcgcgcccg
    21961 cgtgcagacg cacgtcgcgc tgaaacgcca gaccgaacat ctggagcgtc tggcgtggct
    22021 cgacggcctg accggcgccg ccaaccgtcg cagcttcgac cagcgcctcg ccgccgaatg
    22081 gcgccgtgcg ctgcgcaacc gcacgccgct cgggctggtg ctgttcgatc tcgatcactt
    22141 caaggcctac aacgaccgcc acgggcatcc gggcggcgac gactgcctgc gccgcatcgc
    22201 agccctcggc cagcagagca tccgccgccc cggcgacctg atcgcgcgct acggcggcga
    22261 ggaattcgcg ctgctgctgc cggacaccga tctcgccggt gtgcacgggc tggccgagcg
    22321 cctgcgccgg cgtgtcgaga cctgcttcgc ctgcagtggc ggcgactgca atgtccgggg
    22381 tgcccccggg ccgatgacgc tgagcgccgg ctgcgcgagc cgggtgccgg gattcaacga
    22441 agatccgatg acgctgatcc ttgaggccga ccggcgcctg tacgccgcca aggttgccgg
    22501 gcgcaaccgc gtcgtcgcca ccgacgacgc gctgccggcg gcaggcggtg tcgcatgacg
    22561 gcgcaggtcg atgtgacccg tcatcaccgg ccgcgcgtgc tggtcgtcga cgatgcgccg
    22621 gtcaaccggc tggtgctgag cgaggcgctc ggcgaggact acgaggtgct gctcgccgac
    22681 gacggaccgg gggcggtgcg cctcgccggc ggcgaggcgc gcccggacct gatcctgctc
    22741 gatgtcaaca tgccgggcat ggacggctac gcggtctgcg agcagctcaa ggaatcgacc
    22801 cgcacccgtg acatcccggt gatcttcgtc accgcccgca acgaacccga cgacgaggtg
    22861 cgcggtttca cggccggtgc ggccgactac atcacctggc cgctgcagct gccggtcgtg
    22921 caggcgcggg tgcgtacgca gctgtcctgc agcggcctga tccagcgcct cgacgcccag
    22981 aaccgcgtgc tcgccgaacg gctggccgca ctcgccgatg ttggcggcga ggtgccggcg
    23041 cggctgtatc tgcgcgcgct cgaacacacg cgcgacgcgg tgatgatcac gcgcgccgac
    23101 agcacgatcg tcgccgtcaa ccatgcgttc gcggcgctga ccggctatcc ggagtccgaa
    23161 gccctgggcc ggacgccgcg gctgctgcgc tcgggccggc atccgccggc gttttacaaa
    23221 gaactgtatg cgacgctcgc cgcgcgcggc cgctggagcg gcgagctgat caaccgccgc
    23281 cgtgacggct cgctgttccc ggcggtcatg accatcgccg ccgtccacga ccgcgacggc
    23341 gaactcggcc actatgtgtc ggcattcagc gatgtgacga tgatccgcca ggtgcaggag
    23401 cgcatggatt acctgacctg gcacgacgcg ctgaccggcc tgccgaaccg cctgctgctg
    23461 accgaccgcc tgcgccagac gctgaagctc ggccagtgcg acacgcgcgt ggccgtggcg
    23521 atggtgctcg acatcgaacg cttcggggcg ctgaacgagg cgctgggcgc gcaggttgcc
    23581 gatgccgtgc tcggcgtggt cgccagccgc ctgcgacggg agttgtgcac cggcgacacg
    23641 ctggcacgcc tggacggcga cgagttcgcg gtcgtgctcg caggggtgta cgactcgcgc
    23701 gagagtgccg cccatcacgc gcggctggtc gccgggcagc tgggcgctgc agtggccgaa
    23761 ccgctggaaa tcgccggctc ggtgctgcgc ctgggcctcg gcatcggcat cacgctgttt
    23821 ccgaagtacg agaacgacga ggccggcgag gtcctgcgcc gggcgcagac cgcacgtcac
    23881 cgcgccggcg atcccgagac cggcggccag tcgatcgtgt tcttcgacga gcgcatggga
    23941 gagagcgtcg agcatcgctt ccggctcgaa caggaactgc atcatgcgat caatgccggc
    24001 gagctgcgtg tgtacctgca gtcgcaggtc gatcaagacg gccgcatcac cggcgccgag
    24061 gcgctgctgc gctggcagca cccgcagcgc gggctggtgc cgccgggcga gttcatcccg
    24121 atcgccgagt gcacggagct gatcgtgcag ctcgaccgct gggtgctcgg cgaggtcgtc
    24181 gcgctgcttg cccgcagcgc cggctgccgg ccggcaccga cgctgtcggt caacgtcagc
    24241 ccgcgcatgt tcgcaagccc cggcttcgtc gacgaactgc gcacgctgct cgctccgcac
    24301 ccggacagcg cccggcggct ggtcatcgag ctgaccgaag gcgtgctggt gcatgatccc
    24361 gaagtcgtcg ccgagcgcat ggccgcgctc ggggcgcttg gcgtgcgctt ctcgatcgat
    24421 gatttcggca ccggctattc gagcctcacc tacctgaagc gcctgccgct gcacgagctg
    24481 aagatcgacc gcagcttcat cctcgaagca ccgcacgacg ccgacgatgc ggcgctggtc
    24541 gggctgatcg tgtcggtggc cggccacctg cgtctggagg tggtcgccga gggcgtcgag
    24601 accgccgtgc acgccgactg gctgcgcagt catcccggcg tgcgccggca gggctatctg
    24661 tacggccgcc cggtgccggc cggcgactgg ttcggcgact acgcgagcgg ctgcgacgcc
    24721 tgagagaccc tgagagccag ggtgccgggc agcaaaaagg ccggggggaa cccggccttt
    24781 ttttgaagca ggacgttcgg tcggcgggcg acttacttgt acatgctctt cttgaacatg
    24841 cggctcagct tctcgttggt gtccatcgcg atcgacttgg cctcgttggc ggtcgccagc
    24901 gcctggttgg cggtggcctt ggccgactgg gcctcggcgc gcacggcttc gaggtcgctg
    24961 gtgctggcgc agccgacagc gaggacggac aggccggcga gggcggacag cttgatcagg
    25021 ttcttcatgt tcggctccta aacagtcagg caaaaaaaca acgcggacag acatgcccgc
    25081 aacaaagccc cgctagtgta tcgctccggt ttgccttgcc aagccggcga tccgcgccga
    25141 cgacaaataa tgttgcgttg cgcaagcgtc gccggccgca gggccgttca cgcgccgtcc
    25201 ttctgctgcg ccgattcctc ggccagccgc gcggcgtgac gcttggtcag gcgcaggaac
    25261 agcggcgtca ggccggcatc ctcgtacatc ggatcgccct gttcgtcctc ggcgatcacc
    25321 cgcgagccct gcacgtacgg gaacgccgcc tcgacctgat cgagcgcggc cgacagcagt
    25381 tcggtgatga gttcggcacg gctcttcagc ggatacatct cggcgagcgc cgccagccgc
    25441 gcggcatcgt gcagcggcag ctgcacgcag caggattcga ccgtcagcgt gcggacaccg
    25501 gcgcgctccc attcgcggat cagattccgg atgtgcatgg catacctcgg gcgggaaggg
    25561 tcggcgtcag tatggtcgtg gtacggcgcc gctgcatccg gtgacggcag cagcagggat
    25621 gtcacgattt tgacgcacca ctgtcacccg ggccggaata ccgatcggca gtcgccttat
    25681 tgacaaatcc agagcctgtg ccagttttcg accgggcgcg cgaccttaaa ataccgtaat
    25741 gaacaaacgg taatatttgg cgcgtcaatt gcttaatggg gattgtggtc aggacctgtg
    25801 gcgtaaggct gcaggtccgg tccgcaaggc gctcaaaccc ggctacagac caggcccgcc
    25861 gctcaggaga aaacgctcaa tgttaccgct gtggttcgac atgaatgccc gcgtctggct
    25921 gcatgcccag catgtgctgc tcgacaacgt ggtggcgatg atgcggctgg tgtccggtgc
    25981 gcgatgtccg gcgccggtgc gcctcgccga tcgttgcgac ggctgcggtg gtgccctgca
    26041 atggcgtggc ccgcaggcgg cgccgtacca gcgctgtccg gcctgcggcc tgatccgctt
    26101 ccatgcgccg ctgccgcagc ttccggccgg tagcgctatc agcggcgaga cgcatcgcgt
    26161 cctgtccttc cggccccgac gcggcgcctg agccgcagcc ggccgaaaat cagcgtggtt
    26221 cgatcgagac cacgctgcgc agcgaattgg cgatgaagca gttgccgtgt gcgcgcgcgt
    26281 gcagcgcatc gagcgcggag gcgtcctgct cgtggccggg cgcgaagcgc acgcgcggac
    26341 gcagcgtcac gcgcgtgacc atcatgcggc cgtcggcatt gcggccggtc tcggcgctgg
    26401 cctcgtcctc gtaggcatcg acgacgatgc gcttcagggc ggcgatggtc aggaaggtca
    26461 gcatgtgaca gctcgccagc gcaccgacca gcccctgctc gggatcgacc gcgctggcat
    26521 cgccgccgta ttccggcgcc gtcgaggcgc gcacctgttc gccgccgtcg tagacgatgg
    26581 tgtgggtgcg ctcgaaggtc ttcgcatcga agtcggccgt cgcacgctgc cagcgcacga
    26641 cggcccggtg ggcgtcggtc atcgggaggc ttccgtatcg attacttggg gaatacttca
    26701 gcagaccggc ggcacgctgg cgccggcgcg ggccaggcgg tcatgcagcg ggctgctgcg
    26761 cgggaagtgc gcgcgcagga attccatctg gtcggcgagg atgcgccggc cctgcagata
    26821 gacgtattcg gcgtgcgtcg gcgcgaacgg cacggcgagc agcggcatgt gcgcctgctc
    26881 gggcgtgcgc ccgcccttca ggtgattgca gcgccggcag gcggtgacga cgttgctcca
    26941 gcgatcgcgc ccgccctgca ccagcggcgt cacgtggtcg cgcgacagtt cacgcaccgg
    27001 aaagctgtcg ccgcagtaca ggcagacgtt gaagtcgcgc cggaacagcg ccgggttcga
    27061 cagcggcggc acgtaaccgg ggcgcaggcg caggctggcg tagcgcccgc cgaaggtcgc
    27121 aagcacggcg tggaccggga tcaggctgcg ccggccggtg acggcgttgc agccgccgcg
    27181 cagcgtgtac agcagcgtgc cggccgtgaa ggcgacatgc ccggcgcagt gcaggcgcac
    27241 ggcttcctgg tagtcgatcc actccagcgg catgccggag atgtcggtgc gcagcaccgg
    27301 ctgatccaga ccgtacatca tccctcccgt cgtgcagacg tggccgcccg tgtcgcggcg
    27361 gcggatgaag gcacggtgac ggcggcgatc tgcggatgcg cacgcacggc accgtccggc
    27421 cttttgggcc agccgcctag atacaccagt cgacgacgca ggggtagtgc gggcacgact
    27481 ttcgcagtgc aaaagccatc ggataccata tccggaccga ttgcagacgt tttagagcaa
    27541 agactctagt ctgccgcccc tccggcacgc ccggagcggg ccatccgcct tgaacccccg
    27601 gcactcgtcc gtcccgctct gcgggccggt gtgttgccgt ctggagaacg cttacatgac
    27661 cttacgtctg gtggtcccga aagagacctg gacgggcgag cgccgcgttg cgctcgaccc
    27721 cagcgtcgcc gagcgcttcc agcgcctcgg cgccgaagtg cagatcgagc gcggcgccgg
    27781 ctgcggctcg cattttggcg atgcgcaata cgaaaagggc gcgaagctcg tcgacgacat
    27841 cgtcgcggcg ctggccgcgg ccgatgtcac cgtgcgcgtg cagccgccga gcctcgaaga
    27901 ggtcgagcag ctgcgcgacg gctcggtgct gatcggcttc ctcgccccgc accgcaacgc
    27961 cgaggtcatc cggcgcctgc gcgacaagaa gatcacgagc ttcgcgttcg agctgctgcc
    28021 gcgcatctcg cgcgcgcagt cgatggatgc gctgtcctcg caggcggcga tcgccggcta
    28081 caaggtcgcg ctgatggccg cgagcctcgc gagcttcttc ttcccgatgc tgaccacggc
    28141 cgccggcacc atccgcccgg ccaaggtcgt cgtcgtcggt gccggcgtcg ccggcctgca
    28201 ggcgatcgcg acctgcaagc gcctcggcgc gatggtcgag gcctacgaca tccgcgctgc
    28261 ggccaaggag caggtcgagt cgctcggtgc caagctgatc gacaccggcg tcgatgcctc
    28321 cggtcagggc ggctatgccc gcgagctgac caaggaggaa atcgcccgcc aggccgaggt
    28381 gctcgcgcgc cacatcgccg ctgcctcagc ggtgatcacc accgccgcga tcccgggccg
    28441 tccggcgccg aagatcgtca cgaaggcgat ggtcgaggcg atgaagcccg gggccgtcat
    28501 cgtcgatctc gccgccgaaa ccggcggcaa ctgcgaactg accgagcccg gccagaccat
    28561 cgagcacaac gacgtgacca tccacggtcc gctgaacgtg ccgagcctga tcccggtgca
    28621 cgcgagcgag acctacgcga agaacgtctt caatttcctg tccccgttcg tgaagaacgg
    28681 cgagctcgcg ctcgactgga acgacgaggt catcgcgaag agctgcctca cgcacgacgg
    28741 ccagatccgt cacgaaccga cccgcaagct ggtggggtga gcgtcatgat cgaaggtttc
    28801 gccgcgctgt acatcttcat gctcgccgcc ttcaccggct acgagatcat cggccgcgtg
    28861 ccggtcatcc tgcacacgcc gctgatgtca ggctcgaact tcgtgcacgg catcgtcgtc
    28921 gtcggcgcga tggtcgcgct cggccacgcg cagaccggcc tggagcagct gctcggcttc
    28981 atcggcgtcg tgctcgccgc cggcaatgcg gtcggcggct acgtggtcac cgagcgcatg
    29041 ctcgaaatgt tcaagtcgag caaggagtaa gcgaacgtgg ccggactcat ccagatcgtt
    29101 tacttcgcgg ccgcggcgct gttcatcctc ggcctcaagc gcatgtcctc gccgaagacg
    29161 gcgcgcagcg gcatcgtctg ggccggtgcc ggcatggcgc tcgccacgct ggtcaccttc
    29221 ctgtacccgg gcctgtcgaa ctacctgctg atcctggtcg cgatcggcgt cggttcctat
    29281 gcggcctggg tcagcggcaa gaaggtcgcg atgaccgaca tgccgcagat gatcgcgatc
    29341 tacaacggca tgggcggcgg tgccgcggcc tgcatcgccg cggtcgagct gctcaagggt
    29401 ggcagccacg gtgccgtgca tgcgctgctc ggcgtgctcg gtgcgctgat cggcgccgtg
    29461 tcgttctcgg gctcggtggt cgcgtgggcc aagctcgacg gacgcatgaa gaaggcgatc
    29521 cgcttcccga ggcagaagct cgtcaacctc ggcgtgttcg tgctggcgct gctgttcggg
    29581 ctgatcgtca tgggcgcgag ccagcccggt gcgttcagca tcatcgcctt cttcctgctg
    29641 gcgctcgcct tcggcgcgat gatggcgctg ccgatcggcg gtgccgacat gccggtggtc
    29701 atctcgctgt tcaacgcctt caccggtctt gcggtcgcgt ttgaaggcta cgtgatgcag
    29761 aacgcggcga tgatcatcgc cggaaccgtc gtcggtgcgg ccggttcgct gctgacgcag
    29821 ctgatggcga aggccatgaa ccgcccgatc agcaacgtgc tgttctcgaa cttcggcggc
    29881 ggcagcggtg cgacggccga ggaagaggtc aagggctcga tgaaggccag cgagccgcag
    29941 gatgcggcgg tgatgatggc ctacgcggcc aaggtcatca tcgtgcccgg ctacggcatg
    30001 gcggtcgcgc aggcccagca caaggtctgg gagctcgccg agctgctcga agagaacagc
    30061 gtcgaggtca agttcgcgat ccatccggtc gccggccgca tgcccggcca catgaacgtg
    30121 ctgctcgccg aggccggcgt gccgtacgac aagatcttcg acctcgacga gatcaacaac
    30181 gagttcgcgt cgaccgacgt ggtgctggtc atcggcgcca atgacgtcgt caacccggtc
    30241 gccaagtccg atccgaacag cccgatctac ggcatgccgg tgctgaacgc cgaccaggcc
    30301 aggaacgtgc tggtgatcaa gcgcggtcag ggcgcaggct tctcgggcat cgagaaccac
    30361 ctgttctacc tcgacaacac gcgcatggtg tacggcgacg cgcagaaggt gctcgccgag
    30421 ctgatcggct tcgtcaagac gctctgatcg cggcgcggcc cgcagccgga ttcgtcgtgc
    30481 gcgacggccg gccgcggatg ccctccgcac gaggcggcgc tccctgcggg gcgccgcctt
    30541 tttcctgtgc gcgtcagtcc gccggcccgg gcggcggcac gcgcagccag aaggtcaccg
    30601 gtccgtcgtt gaccagttcg acctgcatgt cggcgccgaa gcgcccgctc gcgaccgggg
    30661 cgtgcgcggc ttcggcgagc ccgagcagat gccggaacag ccgctcgccg tcggccggtg
    30721 ccgccgccgg cgtgaagctc gggcgcgtgc ccttctgcgt atcggccggc agcgtgaact
    30781 gcggcaccag cagcaggccg ccgccggtat cggcgaggct gcggttcatg cgcccgtcgg
    30841 catcggcgaa gacgcggtag ccgagcaggc gttcgagcag gcgcgcggcc tgcgcctcgc
    30901 cgtcgccgcg ctcgacgccg accagcacca gcagcccggc cgcgatcgca ccgaccggct
    30961 cgccggcgac cgtcacctgc gcgcgcgtga cgcgctgcag cagcccgatc accggtagtg
    31021 gtcggcgagc gcgtcggtgg cgcgcaccag tgcgtcgatg atgccgggct cggtcgccgc
    31081 gtggccggca tcggcgatga tgtcgagctg cgagcccggc caggcgcggt gcagcgccca
    31141 ggcgccgtcg agcgggcaga tcacgtcgta gcggccgtgg acgatgtagc cggggatgcc
    31201 ggcgaggcgg ctgacatcgc gcaccagctg gtcgggcgcg aggaagctgt cgtgcatgaa
    31261 gtagtggcac tcgatgcgcg ccagcgacag cgccatgtgc ggatcggcga agtgatcgac
    31321 cgtgtgcgga ctcgggcgca gcgtcagcgt cgtgccctcc cagaccgacc aggccttggc
    31381 gcagcgcatg cgctcgacct cgtcatcgcc ggtcaggcgc cggtagtagg ccgagaccag
    31441 atcgccgcgc tcggtctccg ggatcggtgc cagatagtcc tcccaggcct ccgggaacag
    31501 ccgcccggcc ccggactgat agaaccagcg gatgtcctgc ggccggcaca ggaagatgcc
    31561 gcgcagcacc agcccgagca cgcgctccgg gtgggcctcg gcgtaggcga gcgccagcgt
    31621 cgagccccag gagccgccga acaccaccca gcgctcgatg ccgagcgtct tgcgcagcag
    31681 ttcgatatcg gcgatcagcg ccgtcgtggt gttgtcggtc agctcggcgt gcggcgtgct
    31741 ctgaccggcg ccgcgctggt cgaacagcac gatgcgatag cgcagcggat cgaaaaaccg
    31801 ccggtgcacc ggctggcagc cggccccggg gccgccgtgc aggaacagca ccggcaggcc
    31861 gccgggcgtg ccggtttcct cgacgtacag cgtgtgcagc gggctgaccg gcaggcggcg
    31921 cgtcgcatac ggacggatgt cgggataaag ctcgcgcatg tcgcaggctc cgccggaagc
    31981 ggtgccggcc acgggtgaac cggtcgtttc cgggataaga aatttctgat ggtcgattca
    32041 gcggatggcg cagccgaaac cgcctgatgg cgcatggctg tcatgaaaca gctgtataca
    32101 ctgtgcgccc atgcaggagg gaacgggaag cggcagacag gccgcgccgc aggaagggcg
    32161 gcagcgcagg aggcgcggca cggaagcccg aggcaggtcc ggggcatgaa acggtgaggg
    32221 gaacaccgct ttcgggcccg gatcgaggcg gggctgggac acggtgcgga acgcaccggg
    32281 cggtgcacgg ggcaccacgt cagggaagac ggctcaggga gagcgacgtg gaaggcgcgg
    32341 gtcgggatgg cccgtaccgg aagcggtgta tcaccttctc aatgtttggc tgctacgttt
    32401 ttcagccgcc gtcgcgcatg cgatggcggc atttttcttt tggcggtcag tcatccgcgc
    32461 cgcaggcgag cgcccgctcg cgcgaggcgg cgagctcggc cgccgcgtgc ggcgccggtc
    32521 cgccggcaaa gcccggccag ccgctgcaat ggcggcggac cagctgcgcg agcgcgtcga
    32581 ggtgcgccgg cgcgtcgttc agcgccggca cgtagcgcag ccgctcgccg ccggcctgct
    32641 cgaacagcag gcggttctcg atcgcgatct cttcgagcgt ctccagacag tcggccgcga
    32701 agcccgggca gatcacgtcg acgctgcgca cgccggtgcg cgcccactgc tgcagggtct
    32761 cgctggtgta gggcctgagc cactcggcgc gcccgaagcg cgactggaag gccacggccc
    32821 agcggtcgtc ggcgagcccg agccgcccgg cgatcaggcg cgcgctcttc aggcactggc
    32881 agtggtaggg atcgccgagc gcgaggttgc gcttcggcag gccgtggaac gagaacagca
    32941 cgcgctcggc caggccgccg tgggcggcgg cgtgccgatc gaggctcgcg gcgacggcgt
    33001 ccacccaggc cggctcgtcg tggt
//
LOCUS       DAOQIE010000004        32462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_573_length_32462_cov_7.218440, whole genome shotgun sequence.
ACCESSION   DAOQIE010000004 DAOQIE010000000
VERSION     DAOQIE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 32462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32462
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_32462_cov_7.218440"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PK144_00475"
     CDS             complement(<1..1137)
                     /locus_tag="PK144_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNF64226.1"
                     /translation="MSSAALLARRLHGSAAAATAAKPARATPAARAALPGYLQARLRL
                     SQPGDALEQEAERAAAAVARGEAPGVGPTASTTLARSGAGESALPPALEQRIEARRGH
                     GEPLAPAVRTDMEARFGSDFGSVRTHTGPEAADLSRALDAQAFTSGGDVFFDSGRYAP
                     AAPEGRALLAHELTHVVQQGASPAAARQVQRAKGDGGAGTKTKGTKPKSEVRLDGQRV
                     IIPELKISAKKAGRRKPAYAANTELRKGPRKTSQRAVWGQSLKPGVVAGLEKDVKSGK
                     GNFSALNADGSLCGAKLPGGRLLIGSQAAVADELTLPKWTARGASLPMDVDHFLEWQL
                     NGNDALDNYVLGDAALNQAAGSITAAEIDRAVESALNELASDAAN"
     gene            complement(1134..2483)
                     /gene="astB"
                     /locus_tag="PK144_00480"
     CDS             complement(1134..2483)
                     /gene="astB"
                     /locus_tag="PK144_00480"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HNF64227.1"
                     /translation="MSVCEVNFDGLVGPTHHYAGLSWGNVASAANAASRSNPRAAALQ
                     GLAKMRRLTQLGLVQAVLPPQERPDLALLRRLGWRGSDAQVLAAVAREAPRLLAASSS
                     AAAMWTANAATVAPSTDTADGRVHLTPANLIDRFHRALEAATTTRVLRAIFRDPARFV
                     VHDALPAHPWFGDEGAANHTRLAPAHAAAGLHLFVYGRSALGGGPAPQRYPARQTLEA
                     SQAVARLHGLDPAQTLFVQQNPAVIDAGVFHHDVIGVGNETVLLVHEDAFLDRAGTLA
                     ALRARLPALVPVEIARDAVPLAEAVRTYLFNSQLLTLPDGRMHLLLPSECAESPQVSA
                     ALDAILADPANPIATREHLDLHESMRNGGGPACLRLRVVLNEAERAACAPGIFLDDAL
                     YARLCAWVQQHYRDRLHEADLADPALLDDNRRALDELTRLLGLGAVYPFQLDAGAGA"
     gene            complement(2560..3084)
                     /locus_tag="PK144_00485"
     CDS             complement(2560..3084)
                     /locus_tag="PK144_00485"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF64228.1"
                     /translation="MYAGPPEDDAADRARAFRLRSRWRLAAPVEAVWAVLTDVPAWPQ
                     WWPNLCAVEPLAAGAADGTGAVYRYLWRARLPYRLQFTLQVTRVEPPQRLEGRACGQL
                     DGRGVWTLADHGGVTGVEYRWAVDLRHPLLGRLAPLLGPVYAWNHHQVMAAGARGLAQ
                     RLGVRLLGCEGEAL"
     gene            3252..4247
                     /locus_tag="PK144_00490"
     CDS             3252..4247
                     /locus_tag="PK144_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64229.1"
                     /translation="MVAVTAVTVRRTLRALLCCLVLAPVAPLRADGGANLYAALERLP
                     ATPVEVRGATLRVAFADDGFDLPRARLLDWVRRGAEAVAVYYDGFPVRDYRLLIEPVD
                     GRRVAGGTTWAWRGPASRVSVGRMADEAVLAHDWVLVHEMVHTALPLLDRRHHWLEEG
                     IASYVEPLARAQAGQLSEAQVWAQFVGGMPLGLPQDGDEGLDRTHTWARTYWGGALFC
                     LLADVAIREESGGRAGLREALRAIAKDSRGMADEWPIERVLAAGDAATGTGVLTRLYA
                     THASAAVSPDLPALWRALGVVAQENAEPRFDDSAPLAAIRRSLTAPAPAGVLSRP"
     gene            complement(4234..6882)
                     /locus_tag="PK144_00495"
     CDS             complement(4234..6882)
                     /locus_tag="PK144_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNF64230.1"
                     /translation="MTIRNLDAVFKPRSVAVIGRGSKDDAPDAQLVQNLIRQGFNGPI
                     MPVNPDRKALRGILCYRDLASLPLAPDLAVITTPMAGAPEQIRTLGELGTRAVLLLNE
                     EMLKSWDDGGTAIRQALLDAAKPHVLRIAGPDSFGLAATRGGLNVTRGHTPILPGSLA
                     LISQSCAITRSVLDWAHGHNIGFSHVLSLGAKIDVDFGDMLDYLARDGNVKAILLYLE
                     SINDARKFMSAARAAARIKPTIVLRPGRYRANPNEDAVYDSAFRRAGILRVRTIEQLF
                     NSVEALGTAKPVYNNRLAVIGNSRAISLLATETLEAAGGQLAVFNEDTATALGKVVPR
                     GTRIENPLDLGDAANPERYIAVLDALLEAPGVDCILVLHSPLSPELDLASAKAVADRA
                     AKSRRLVITSWLGAASAEPARAVFKQAHVATYDNPGDAVSAFTRIAQHRHNLELLMET
                     PPSIPEAFKPDAERARALLQQALAAGRMRLDAYESMQVLDAYGIPTVPTRVARTPEEA
                     AAIYPEFGHVVVLKLLSPDIASKTEVGALALWLDSAEAVRSEAEAMIARVRTHVPEAR
                     IEGFVVQPMLRRGGAYELAIGMRTGDTFGPLIRFAHGGTEADVIDDKAWALPPLNMAL
                     AQEMIGRTRISQRLKSGLARAADLDALALALIKISQMAIDLGELWELDINPLRVSGKG
                     IIALDATIQVRAFTGKRPRDRLAIRPYPSELEQDFTLPDGRGLRLRPIVPEDEPALQE
                     LVRRMPPDELRLRFFQPIRELSHAMAARLTQLDYYREMALIVAARDVPGRAEIYGVVR
                     ITCDPDMDRAEYAILVDHGMTGLGLGPMLMRRIIDYARRRGIQEIYGEVLWENEGMLK
                     LCRALGFAVKPSADDPGIMHVSIKVD"
     gene            7223..8917
                     /gene="acsA"
                     /locus_tag="PK144_00500"
     CDS             7223..8917
                     /gene="acsA"
                     /locus_tag="PK144_00500"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNF64231.1"
                     /translation="MPDHARARAHFDWAEVRAGLAGLPGGGLNIGWEALDRHVADGNG
                     ARLALRWLGKDGAVRDYTYAQLLAEAQRFACVLERLGVGPGECVAVLAGRIPELYIAA
                     LGALRAGIVLCPLFSAFGPEPVHTRLEKSHARVLVTTVTLYQRKIAALRDTLPALDTV
                     LTVGGDCPGTFRFEALMAQAETGCPVAATGPDSPALLHYTSGTTGSPKGALHVHGAVA
                     AHAWSGRAVLDLRPGDVYWCTADPGWVTGISYGLIAPLVNGVTMIIDEAEFDAQRWYR
                     ILDEQQVSIWYTAPTAIRMLMKLGDAPMAGRTLPALRLMASVGEPLNPEAVLWGQRVF
                     GQPFHDTWWQTETGAIMVANYGSEPVAPGSMGRPLPGIDAAIVHRRRDGGLDFLPDGE
                     AGELAIRAGWPSMFRAYLDEPERYASCFADGWYLSGDRAQRDADGRYWFVGRADDVIK
                     TSGHLIGPFEVESVLLEHPAVAEAGVIGKPDPWAGELIKAFVALKPGHTGDDALRKSL
                     IAHARTRLGPAVAPRELGFVDSLPKTRSGKILRRLLKARELGLPEGDLSTLEGHES"
     gene            9131..9379
                     /locus_tag="PK144_00505"
     CDS             9131..9379
                     /locus_tag="PK144_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF64232.1"
                     /translation="MTVEQIRATILRVLSEVAPNADVSDVNPNVSFHDQFEIDSIDFL
                     RLMTSLEKELQVTILDFDYPKLSTLQGCEDYLRPQLAA"
     gene            complement(9440..10063)
                     /locus_tag="PK144_00510"
     CDS             complement(9440..10063)
                     /locus_tag="PK144_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNF64233.1"
                     /translation="MTSFARVFTDRVRTSELLEGYAVHLRGERVALGTLVALLGKRSF
                     GLLLLLFALPNLVPLPPGSSTVFGLPLLLLCAQMMYGRSLPWLPGWLLKKSIPRSSFD
                     SMLARTLPWLRRIERWLQPRLTQCTGRCAERLLGGWCAVQAIVLALPIPLANFPPALC
                     IVLIALGMLERDGVLVIAGSLIGLISLGIALTVGYGLVQGLLLIGQA"
     gene            10248..10829
                     /locus_tag="PK144_00515"
     CDS             10248..10829
                     /locus_tag="PK144_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNF64234.1"
                     /translation="MQSLRVILVDPSSTRSAILEQALRDAGLQVIGRFAECDMLVDRI
                     SELQPDIVLVDIESPDRDTLESLQTVGRDNPRPIVVFAEDDSPDAIAAAVRAGVSAYV
                     VDGLQPHRVRPIVEVAIARFREFQALRRELEATRSQLAERKLIDKAKGLLMEHRNQTE
                     AEAYASLRKLAMDRNQRIAEVARNVIDVFELLG"
     gene            11049..11576
                     /locus_tag="PK144_00520"
     CDS             11049..11576
                     /locus_tag="PK144_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64235.1"
                     /translation="MAVSLRLLETADAPRIQHLVSDPRLASWANLPRPYPADGAERFL
                     QLADCQQRRGTGRHFAIERFGLLVGVISVGCGTDPGLAMLGYWIGVPWQGLGVASRAL
                     DQLLNLDPLAGPGLRTWGSVCLERNLASRRVLEKNGFTAVAARPYEGPYPHRFAGQTC
                     LTYLRMPNPNAVLIA"
     gene            11733..13745
                     /locus_tag="PK144_00525"
     CDS             11733..13745
                     /locus_tag="PK144_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64236.1"
                     /translation="MEPPLPPQSATVPADDDLPPLLLLVDDDALARLALEAMLAGDGY
                     RLETAADGSEALVLAARLLPDLILLDVLMPGMDGYEVCRRLRADPALAEVPVLMLTAL
                     DDAASRLLGLDAGADDFIAKPADRAELRARVRSITRLNRYRRARERERRLAESQRQQL
                     HSLALLQCVIDNSPDAFLALDENGEIVEWSAQAETMFGWSRAEVLGLDLNDLIVPSHL
                     RVQQRRLHEAVRQTGRFDGFGRVEERVSVRRDGSEFPCEVLLTALPTDRGWRLAGFVR
                     DIGERRDAEIRLRQAEKLEAIGLLTGGLAHDFNNLLNMILGGLELLAPRLPAGDARAL
                     RHHDLALRAARRAAEVTRALLQVARRQPLDAREVDVAAHCIELLPLIRQSAGSGVRIV
                     LHGFEPAPQPPLRACVDAGGLGNALLNLVINARDAMPGGGTLTLEVQLQTLPAGAVPE
                     LAAGTWIVIAVTDTGCGMSPAVRARAFDPFFTTKHRGQGTGLGLAMVLGYARQSGGNV
                     TLAGAAGGGTTVRLYLPAVAPMSPRPEAALPVPGVAAPAGQGERVLLVDDESGLLELG
                     REWLEADGYRVDTCSDPQQALARLQAGGHALLVTDVTMPGPLDGCALAAHACRQQPGL
                     RVLLISGYADDDALAELSRWPLLLKPFTAVELLQAVRRRLDQPGGG"
     gene            13841..15043
                     /locus_tag="PK144_00530"
     CDS             13841..15043
                     /locus_tag="PK144_00530"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNF64237.1"
                     /translation="MNPDHARRPRLLVVDDEADNREFFAETGDLAGYAVASLGDVREL
                     ESALQPAPDVILLDLIMPHLDGIEVIRELARAGCRSRLILVSGCERRVLQAALRLAQG
                     FGLDVLGELQKPVSVAALTALLLQPGMPPARVQPLQPVTLDELHAALAENRFTVHYQP
                     QVALHDGRWCGVEGLVRWCHPQHGLLLPDSFVPLVERGGLGLELTRCVLECALADLAH
                     LHGLLPGGRLPGLSVNLPGEALFDVSLPEQLLALVDAAAWQHDAITFEITETSLAREP
                     VRALDILTRLRLKGVQLSIDDFGTGYSSFGQLHHLPFSELKIDLGFVRVADRDPVARA
                     IVENSIRLGRQLGLTVVAEGVESATLWHWLREQGCDLAQGYLVSRPMPAEQLPAWYAA
                     WGAPACPA"
     gene            15438..15659
                     /locus_tag="PK144_00535"
     CDS             15438..15659
                     /locus_tag="PK144_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64238.1"
                     /translation="MFPADMPDAVCACSRPFLQGHASAAEPVFPVDRDLEHLYVQAAH
                     RARLLGQAQFEAIARHAHPDPLRSHRHAD"
     gene            complement(15689..16267)
                     /locus_tag="PK144_00540"
     CDS             complement(15689..16267)
                     /locus_tag="PK144_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64239.1"
                     /translation="MTEPRPDTPDCASPPCLAHELDPLWSGRAGYAVPLRAPVRAEHS
                     YVQRLCATPAQVLPLLCPVRETDWIADWRPQYVISASGLAEPDCVFATPAPAGGSALW
                     YVTRHDAVQGYVEMVRFVPEHTACRLEIWLQADGDSACLATVRYRLTSLGSVAGDAAV
                     AAYTAEHYLVFMRDWERRLNHWLRTGRCLPAD"
     gene            complement(16296..16676)
                     /locus_tag="PK144_00545"
     CDS             complement(16296..16676)
                     /locus_tag="PK144_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNF64240.1"
                     /translation="MSRTDRTLPLVYSCSGCSSAAQLANHVALTLDRRGLAEMSCIAG
                     VGGGVPALVRVAQGTRPVIALDGCALACVRHCLARAGVSAGTYHLLSDHGVRKRHGAD
                     FDAADAARVLEIVIAGLPQAATAA"
     gene            17049..19211
                     /locus_tag="PK144_00550"
     CDS             17049..19211
                     /locus_tag="PK144_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF64241.1"
                     /translation="MSELSQTPAGARRLPLARYLWQSYLRAAIIPLLVIELGFIGVYW
                     VSSFITYERNAEALGAISREDLARTAQREADAIAEKLGAVGDLTRVFAAETARALRTP
                     APAAATDDDARHAYGADGVYYTTREGVSAGFYSGIVPVGPAERDKVRRTAAIDPLMQA
                     IKAANPLVAQLYLNTHDSYNRIHPYFEVLTQYPAKMDIPSYNFYYEADAAHDPERRAV
                     WTDAYVDPAGSGWMVSSIAPVYGGADGSFLEGVVGIDVTVDTIVRRVLNLQLPWQGYA
                     LLVGRDGTILALPPRGERDFGLKELTAHSYDEAIRADTFKPDDFNVHKRADLAVLGRA
                     LKAGGDGVVELALGSGRTLAGWSTVAGPGWQLVVLAPEHEILAGATALRERLSLVGWL
                     MIASLMLFYVGFLIYLRSRARAMTARVAAPLGDIEALIRRIGGGDYEQSEPDYRIAEL
                     QTVGTHLVDMGRRLGAAYAAMVEAERSVRAALDKERELGEVQRQFINVVSHEFRTPLT
                     IIDGVAQTFERRADRLGPDKIIERARSLRASVRRLVDVLESALSFSRLESSQLTHAFG
                     PVAFAAVLQEVVQTETVAYPQHRFEIDGALPATVHGDAGLLRVMLSAVLDNAARYSPQ
                     AGTVTIRASTGTDDCVHLDIHDQGPGIPADELPRVRERFYRGSAGLASHGAGLGLYLV
                     EQFVQLSGGRLDIDSPPGQGTVVRLVLPVHGPRAAAAA"
     gene            19229..20917
                     /locus_tag="PK144_00555"
     CDS             19229..20917
                     /locus_tag="PK144_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64242.1"
                     /translation="MDTNGAVILCVEDEAEIRRDLVDELLDAGYEVHEAGSGSEAIGR
                     LAGLQPDLILCDVNMPGLSGLQLLEQVRARGDALGEVPFVLLTAYGDREHQIRGRALG
                     ADDYLIKPVDYDLLLATLASRLRNARRVHARVQSRIGDLESQLAGVERRTATLPDRMA
                     LQAALADGRGRRRRGLLVASFDELSRHVQQFGSSFQDEAEQALIGQVQLLAPAQAYRL
                     DSETFGLLFADADALDVSALGRLVEIECRSPGGAAPVRLSTTLCYVHCEPEAGCDVAD
                     RLEDACLAVRFGRRDGGHTLLRLDAAAVQRLRMAGHVEDHLLRALRDGEFTLHFQPKL
                     QLADDRLVGAEALVRWHSRDLGPISPGVFIPIAERCNLIEALSVWVLDEAARAAAVLA
                     REGLDLAIAVNASGVELHAGFPARVREVMAAHGLGAGQLDIELTETSVIADIRHAAGI
                     VAELRGLGVGVAIDDFGTGYGSLSYLRALPVDTVKIDQSFVRGLPGNAIDRQIIESVL
                     TLAHALGIGTVAEGVETAEQEREMRRLGCGQIQGYHLARPMPLTELIRFVRERR"
     gene            20952..21326
                     /locus_tag="PK144_00560"
     CDS             20952..21326
                     /locus_tag="PK144_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64243.1"
                     /translation="MTPAGTTLLVVEDEQELREYLVEELEDLGYQVCEAGNGALALAL
                     LRERPVDLVLTDIMMPQLGGAGLYHATRCDPALAQLPFLFLTALSSAEMLATLPESEQ
                     PHILKKPVDYDELADAIRARLA"
     gene            complement(21341..22627)
                     /locus_tag="PK144_00565"
     CDS             complement(21341..22627)
                     /locus_tag="PK144_00565"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNF64244.1"
                     /translation="MSDFAPRRVAVVDGLRTPFCRAGAEYAELSNLDLLSAVLGALGK
                     RHDLRRDGIDEVWAGAVNSHAKDWNLAREAVLSAGLAPDTPAVTLTQACATSLQAAGV
                     LAAKIATGEIDSGIAAGSDTASDLPIVFDRRFAQRLIRTQRARSLRERLAQWRGFRLR
                     ELLPVAPSPAEPRTGLSMGQHTERMAREWGITRAEQDAFAADSHRKALAARAAGWFDA
                     ELVPCAGVVSDRLPRAGSDAEQLARLRPAFAPDGTLTAGNASALTDGAAAVLLCEART
                     AARRGLPVLAHWVAQQSAAVDFVAGEGLLLAPALAVGRLLKRLGLGFADFDVIELHEA
                     FAAQVLCTLAAWRSADWCRARLGLEAALGEPDPARLNACGSSLALGHPFAATGARVLL
                     SAARRLAAGGGRRALLAVCTAGGMGVAVVLERPHAG"
     gene            complement(22667..24616)
                     /locus_tag="PK144_00570"
     CDS             complement(22667..24616)
                     /locus_tag="PK144_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF64245.1"
                     /translation="MTSDAVWQHWQLRHDPDGIAWLTLDCAGDGPNTLGRAVLEEFGQ
                     VLDALDAAPPRGLVLRSGKPAGFAAGADLREFDGYRDADEVASLIRHVHGLFARLEAA
                     PWPVACVIHGHCLGGGLELALACHWRVAHDLPSTRLGFPEVRLGIYPGFGGTLRATRR
                     LGGPAALGLMLTSRTLAARAASAAGLVDAVAGTHESPDWLARRAVLAGRRSRPGWLAQ
                     LSNSAAARAVIAPLIRRQTAQKVRADHYPAPFRLIEHWRLDGGDAARMLEGEAREVAG
                     LLTGEVSRNLRRVYFATEQLKSLGRQNAAPVAHRLHVEGAGVMGGDIAAVAALAGLEV
                     TLHDLDAARIDAALARARGLFARRLKTPAERLAAAGRLIADPAGDGVARADVLIEAVI
                     EDAGVKRTLFAALEARLPAHALLATNTSALPLETLASALTDPGRLIGLHFFNPVPRMP
                     LVEVVHGEASTPAAVARGCALAVQLGKYPLPCRSQPGFLVNRVLAPYLLAALRLLLAG
                     TPASTLDAAAVGFGMPVGPIELADTVGLDVCRHVLVTLGPAEPAAAAALDARIAAGRL
                     GRKSGQGFYVWKDGKVRRPAAATGTPAELAALADAMLAPLLDACERALADGVVASAEQ
                     LDAGVILGTGFAPFRGGPLRYRGLA"
     gene            complement(24613..27249)
                     /locus_tag="PK144_00575"
     CDS             complement(24613..27249)
                     /locus_tag="PK144_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNF64246.1"
                     /translation="MSTAIAGLILLLLAGLALAAARVSLPVATAVLGAGLLLLDALAD
                     GVRGLWLAWLPLLAWNLTPLRLRLLAPLPDHVRRAQPPMSDTEREALAAGTVWWEAEL
                     FAGDPHWQRLLSLPAPALSAEEQAFLDGPTTALCAQIDDWRITHVDRDLPPALWQTIR
                     TQGFLGMIVPRAYGGLGFSAQAHSAVVTRIASRSVSVAVTVMVPNSLGPAELLLAYGT
                     QAQKDHWLPRLARGEDIPCFALTGPEAGSDAASIPDRGVVCRGLHAGRETLGIRVSWD
                     KRYITLAPVATVLGLAFRLHDPDGLLGDRADIGITLALIPTTHPGVEIGRRHFPGNQA
                     FMNGPTRGRDVFIPLDWIIGGPACAGHGWTMLMECLAAGRGISLPANGTAGALYAARV
                     SGAYARVRRQFKLPIGRFEGVAEALARIGGHAYVLEAARRLSTLALDLGEKPAVVSAI
                     VKYQSTERMRAAVNDAMDIHGGKGICHGPSNWLAGVYQALPIGITVEGANILTRTLIV
                     FGQGALRCHPWLRSELAAAGNPDRRAALAAFDQAFRGHLGHLLGTLARAGGRALSAAA
                     FAPAPAGADAATRHWYRELSRLSAAFALVADVALLTLGGELKRRERLCGRLADALAEL
                     YLASAALKRYEDDGRPEADLACLDYVLAEACYAIEKRLSDALANFPRPLLGSLLRRLV
                     FPWGRRMRGARDAQSARVAELMMSPGPARERLSGPIHVSRDPAEAVGALELAFEAVVA
                     AEAIEARLQAAKRRGRLQAPDPAAALAAGELTAGEFAQLEQAAALVRRVIAVDDFDPA
                     ALAGHGVATVATGPELPPPPSGPEIPESTPPERPAEPSPPEAPEPPATPEVPPPPSGP
                     EIPPSAPEPAGLRPAAFAGARA"
     gene            complement(27556..27927)
                     /locus_tag="PK144_00580"
     CDS             complement(27556..27927)
                     /locus_tag="PK144_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64247.1"
                     /translation="MRALRVVLLYAVLAAGCGNPAVESYVDVAPGELAAVLAAHAERR
                     ERIGYRVRMRSGVLPANETELEDLCADWQLYRRRIKRLIRTGDENGLLTAQSRLQQTE
                     HWLDAYDPRDVEAMKNWIRRR"
     gene            28447..29589
                     /locus_tag="PK144_00585"
     CDS             28447..29589
                     /locus_tag="PK144_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64248.1"
                     /translation="MVASEAGAGWFLEGVSDQGGHWLVRITGTPFIVGRVESCDLYLN
                     DTGVSRQHARLEFDGAGRPLLSDEGSTNGTFVNYQRLEARAPQSLNDGDIVHFAKMAF
                     RLVHREQSKAPAPVVEGIEGGTMFQQVAALPETFTAAAHEKLFFDLLAKGAVSPWFQP
                     IISLRHGKQVVAYELLGRGAQPGLSSSPAELLKIAHATGRTVELSDLFRKTGIRRAQE
                     LGLSRVFFNTMPAEMSIPLLRRLLGELRVMAPVLPMVMEVHEQAIADIELMRDVRALL
                     GELNMKLAYDDFGAGQARLVEIMEVPPDVLKFDIGLVRGISSKPQRVQTMVADLVRMA
                     AGMGVRTLAEGIEAQEDSDFCEAAGFDLIQGYLYGRPTPDPLPVAG"
     assembly_gap    29757..29856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29857..30287)
                     /locus_tag="PK144_00590"
     CDS             complement(<29857..30287)
                     /locus_tag="PK144_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64249.1"
                     /translation="MRRPIVLLRSACLSLGLCLSPPVWAAPPPALLEQVQRLSELLRD
                     GYASWYREATETQTLQPDAGQTLLLVLFTVEGFGGGNRHTQYLAAFEAGEGAGGKPYY
                     TLLDVIAIGGKGWRAIEKLQARAARGRTPGEIVLSLPAKVVG"
     gene            30468..31898
                     /locus_tag="PK144_00595"
     CDS             30468..31898
                     /locus_tag="PK144_00595"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNF64250.1"
                     /translation="MQSNLILDTDSYKASHWLQYPPGTTQVYAYIEARASHVDGVDAT
                     LFFGLQAFLKTYLSRPVGADDLAEARELFAAHGEPFNLDGWQHILDRHGGWLPVEIRA
                     VPEGSRVPLRQALVTIENTDPAVPWLTSYLETALLRAIWYPTTVATISRTAKETLRAA
                     LERTSDDPAASLAFKLHDFGARGVSSAESARLGGMAHLVNFMGTDTVEAIRAIRTCYG
                     PADGMPAFSVPAAEHSTMTMWGEAGELDAYRNMVRRFGRPGAIYSVVSDSWSLARAVD
                     EHWGRTLRDEVLAAGGTLVVRLDSGDPIDSIRYALESLGRSFGFSVNRKGYRVLDGVR
                     LLQGDGIDLSSLAHLCTAIADAGWSLDCFGTFGMGGALLQKVNRDTFSFAQKASWGVV
                     DGEARAICKRPQGDPGKASKAGRIASHRHRGDGRWCTAIEGTLDAGEWQPMLEPVWRD
                     GKLLREQDFADVRARAEAGFGRSAFP"
     gene            32023..32310
                     /locus_tag="PK144_00600"
     CDS             32023..32310
                     /locus_tag="PK144_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64251.1"
                     /translation="MTDMDPAIVERFVYDWCTAEARGTPSARALAAIYASTYMADARL
                     LGFVDLRALDETRLNWALNLIRAYVEGDFTAPWPRAVALCALYDLIPAEAA"
BASE COUNT         4536 a        11372 c        11812 g         4642 t
ORIGIN      
        1 gttggctgcg tcgctggcga gttcgttcag cgccgattcg acggcccggt cgatttcggc
       61 ggcggtgatg ctgccggcgg cctggttcag cgccgcatcg ccgagcacgt agttgtcgag
      121 cgcgtcgttg ccgttcagtt gccattcgag gaagtgatcg acatccatcg gcagcgaagc
      181 gcctctggct gtccacttcg gcagcgtcag ttcgtcggcg accgcggcct gcgaaccgat
      241 cagcagccgg ccgccgggca gcttcgcgcc gcacagcgag ccatcggcgt tcagcgccga
      301 gaagttgccc ttgccggact tcacgtcctt ttcgagtccg gcgacgacgc cgggcttcag
      361 cgactggccc cataccgcgc gctggctggt cttgcgcgga cccttgcgca gttcggtgtt
      421 ggccgcatac gccggcttgc gccggccggc cttcttcgcg ctgatcttca gctccgggat
      481 gatcacgcgc tggccgtcga gccggacctc ggacttcggc ttcgtgccct tggtcttggt
      541 gccggcaccc ccatcgccct tggcgcgctg tacctgccgc gccgcggccg gcgaggcacc
      601 ctgctgcacg acgtgcgtga gttcgtgcgc aagcagcgcc cggccttcgg gtgcggccgg
      661 ggcgtagcgg ccgctgtcga agaacacgtc gccgccgctc gtgaacgcct gcgcatcgag
      721 cgcgcggctc aggtccgctg cctcggggcc ggtgtgcgtg cgcacgctgc cgaagtcgct
      781 gccgaagcgc gcttccatgt ccgtgcgcac ggccggggct agcggctcgc cgtggccgcg
      841 gcgggcctcg atgcgctgtt cgagcgccgg gggcagtgcg ctttcgcctg cgccgctgcg
      901 cgcgagcgtc gtcgaggcgg tcggcccgac gccgggcgct tcgccgcggg cgacggctgc
      961 ggcggcgcgt tcggcctcct gctccagcgc atcgccgggc tggctcaggc gcagccgcgc
     1021 ctgcagatag ccgggcagcg cggcgcgggc cgccggtgtg gcgcgtgcgg gctttgcggc
     1081 ggtggccgcg gcagcgctgc cgtgcagccg gcgggcgagc agggcggcgc tgctcatgcg
     1141 ccggcacccg cgtcgagctg gaacggatac acggcgccga gcccgagcag gcgcgtcagc
     1201 tcgtcgagtg cgcggcggtt gtcgtcgagc agcgccggat cggcgagatc ggcctcgtgc
     1261 aggcggtcgc ggtagtgctg ctgcacccac gcgcagagtc gtgcgtacag cgcgtcgtcg
     1321 aggaagatgc cgggcgcgca ggccgcgcgc tcggcctcgt tcagcaccac gcgcaggcgc
     1381 aggcaggccg ggccgccgcc gttgcgcatg ctctcgtgca ggtcgaggtg ctcgcgggtc
     1441 gcaatcgggt tggccggatc ggcgaggatc gcatcgagcg cggcgctgac ctgcgggctc
     1501 tcggcgcatt cggacggcag cagcaggtgc atgcggccat cgggcagcgt cagcagctgc
     1561 gagttgaaca gatacgtgcg cacggcctcg gcgagcggca ccgcgtcgcg ggcgatctcg
     1621 accggcacga gcgccggcag ccgtgcccgg agcgcggcca gcgtgccggc gcgatcgagg
     1681 aaggcgtcct cgtgcaccag cagcaccgtt tcgttgccga cgccgatcac gtcgtggtgg
     1741 aacacgccgg catcgatgac cgccgggttc tgctgcacga acagcgtctg cgccggatcg
     1801 aggccgtgca ggcgcgcgac cgcctggctg gcctccagcg tctggcgggc cggatagcgc
     1861 tgcggggccg gaccgccgcc gagggcgctg cgcccgtaca cgaacagatg caggccggcg
     1921 gcggcgtgcg ccggcgcgag ccgggtgtgg ttggcggccc cctcgtcccc gaaccacgga
     1981 tgcgccggca gcgcgtcatg cacgacgaag cgcgccggat cgcggaagat cgcgcgcagc
     2041 acgcgcgtcg tggtcgcggc ctccagcgcg cggtggaagc ggtcgatgag gttggccggc
     2101 gtcaggtgca cacggccgtc ggcggtgtcg gtcgagggcg cgacggtcgc ggcgttggcc
     2161 gtccacatcg ccgcggccga gctgctggcg gcgagcagcc gtggtgcctc gcgcgcgacc
     2221 gcggccagca cctgcgcatc gctgccgcgc cagccgaggc ggcgcagcag cgccaggtcc
     2281 ggccgctcct gcggcggcag caccgcctgc acgaggccga gctgcgtgag ccggcgcatc
     2341 ttcgcgagtc cctgcagcgc cgcggcgcgc gggttcgacc ggctcgcggc gttggccgcc
     2401 gaggcgacgt tgccccagga caggccggcg tagtggtggg tggggccgac gagaccgtcg
     2461 aagttgactt cgcacacgga catggcggac ctcggcaggc gcggcggcaa ccgcgcaagt
     2521 ctggctggcc ggcgggcgct ggcaagagcg ggcgccgcac tacagggctt cgccctcgca
     2581 gccgagcagc cgcaccccga gccgctgcgc caggccgcgg gcaccggcgg ccatgacctg
     2641 atggtgattc catgcgtaga ccggaccgag cagcggcgcg agccgcccga gcagcggatg
     2701 gcgcagatcg accgcccagc ggtactcgac cccggtcacg ccgccgtggt ccgcgagcgt
     2761 ccagacgccg cggccgtcga gctggccgca ggcgcggcct tcgagccgct gcggcggctc
     2821 cacccgcgtg acctgcagcg tgaactgcag ccggtagggc aggcgggcgc gccagaggta
     2881 gcggtacacg gcgccggtgc catcagcggc accggccgcg agtggctcga ctgcgcagag
     2941 gttcggccac cactgcggcc aggccggcac gtcggtcagc accgcccaga ccgcttcgac
     3001 cggagccgcg agccgccagc gcgaacgcag ccggaaggcc cgggcccggt ccgcggcatc
     3061 gtcttcaggc gggcctgcgt acatcgccgc tgcggtctcc ggtctcccgg tcgtgccggg
     3121 ctttcagtat cgtgccgcgc cgcgggcgtc cgcagccgtt tcgccgcgcg ggcaccgggc
     3181 atcgcccggc cccgtgggcc agccgggcgt gtcgcccgtt ccgaacaccg tagtccccgg
     3241 gagtcaccgc gatggttgcc gtcaccgccg tcaccgtgcg ccgcactctg cgcgcgctgc
     3301 tctgctgcct cgtgctcgcg ccggtcgcgc cgctgcgtgc cgacggcggt gcgaacctct
     3361 atgcggcact ggagcggctg ccggcgacgc cggtcgaggt gcggggcgcg acgctgcgcg
     3421 tggccttcgc cgatgacggc ttcgacctgc cccgggcccg gctgctcgac tgggtgcgcc
     3481 gcggggccga ggcggtcgcg gtctattacg acggctttcc ggtgcgcgac taccggctgc
     3541 tgatcgagcc ggtcgacggg cgccgggtcg ccggtggcac gacctgggcc tggcgcgggc
     3601 cggcgagccg tgtctcggtc gggcgcatgg ccgacgaggc cgtgctcgcg cacgactggg
     3661 tgctggtgca cgagatggtg cacacggcac tgccgctgct cgaccggcgc catcactggc
     3721 tggaggaagg catcgccagc tacgtcgagc cgctggcgcg cgcgcaggcc gggcagctca
     3781 gcgaggcgca ggtctgggcg cagttcgtcg gcggcatgcc gctcgggctg ccgcaggacg
     3841 gcgacgaggg gctggatcgc acgcacacct gggcgcgcac ctactggggc ggggcgctgt
     3901 tctgcctgct cgccgacgtg gcgatccgcg aggaatcggg cggccgcgcc gggctgcgcg
     3961 aggcgctgcg ggcgatcgcg aaggacagcc ggggcatggc cgacgaatgg ccgatcgagc
     4021 gggtgctcgc ggccggcgat gccgcgaccg gcaccggcgt gctcacgcgc ctgtacgcca
     4081 cacacgcgag cgcggcggtc agccccgacc tgccggcgct ctggcgcgcg ctcggcgtcg
     4141 tggcgcagga gaacgccgag ccgcgcttcg acgactcggc gccgcttgcg gccatccggc
     4201 gcagcctgac cgcgccggca ccggccgggg tgctcagtcg accttgatgc tgacgtgcat
     4261 gatgccggga tcatcggcgc tcggcttgac cgcgaagccg agcgcacggc agagcttcag
     4321 catgccctcg ttctcccaca gcacctcgcc gtagatctcc tggatgccgc gccggcgtgc
     4381 gtagtcgatg atccggcgca tcagcatcgg gccgaggccg aggccggtca tgccgtgatc
     4441 gacgaggatc gcgtattcgg cgcggtccat gtccggatca caggtgatgc gcacgacgcc
     4501 gtagatctcg gcgcggcccg gcacgtcgcg cgcggcgacg atcagcgcca tctcgcggta
     4561 gtagtcgagc tgggtcaggc gcgcggccat cgcgtgcgac agctcgcgga tcggctggaa
     4621 gaagcgcagg cgcagttcat cgggcggcat gcgcctgacc agctcctgca gcgccggctc
     4681 gtcctcgggc acgatcgggc gcaggcgcag gccgcggccg tcgggcagcg tgaagtcctg
     4741 ctccagctcg ctcggatagg ggcggatcgc cagccggtcg cgcgggcgct tgccggtgaa
     4801 ggcgcgcacc tggatggtcg catcgagcgc gatgatgccc ttgccggaga cgcgcagcgg
     4861 attgatgtcg agctcccaga gctcgccgag atcgatcgcc atctgcgaga tcttgatcag
     4921 cgccagcgcc agcgcatcca ggtcggcggc gcgggcgagg cccgatttca ggcgctggct
     4981 gatgcgcgtg cggccgatca tctcctgcgc gagcgccatg ttcagcggcg gcagcgccca
     5041 ggccttgtcg tcgatgacgt cggcctcggt gccgccgtgc gcgaagcgga tcagcggccc
     5101 gaaggtgtcg ccggtgcgca tgccgatggc gagttcatag gcgccgccac ggcgcagcat
     5161 cggctgcacg acgaagcctt cgatgcgggc ctccggcacg tgcgtgcgca cgcgcgcgat
     5221 catcgcctcg gcttcgctgc gcacggcctc ggccgaatcg agccacagtg ccagcgcgcc
     5281 gacctcggtc ttgctggcga tgtcgggcga cagcagcttc agcacgacga cgtggccgaa
     5341 ctccggatag atcgccgcgg cttcctcggg cgtgcgcgcg acgcgcgtcg gcacggtcgg
     5401 gatgccgtag gcatcgagca cctgcatcga ctcgtaggca tcgaggcgca tgcgcccggc
     5461 ggcgagggcc tgctgcagca gcgcgcgcgc acgttcggcg tcgggcttga aggcctcggg
     5521 gatcgacggc ggcgtctcca tcagcagttc gaggttgtgg cggtgctggg cgatgcgcgt
     5581 gaaggcgctg accgcgtcgc cggggttgtc gtaggttgcg acgtgcgcct gcttgaacac
     5641 cgcgcgtgcg ggctcggccg aagccgcacc gagccagctg gtgatcacca gccgccggct
     5701 cttcgcggcg cgatcggcga cggccttcgc gctcgccaga tcgagctccg gcgacagcgg
     5761 cgagtgcagc accaggatgc agtcgacgcc cggggcttcg agcagcgcat cgagcacggc
     5821 gatgtagcgc tcggggttgg ccgcatcgcc gagatcgagc gggttctcga tgcgcgtgcc
     5881 gcgcggcacg accttgccga gcgcggtggc ggtgtcctcg ttgaagaccg cgagctggcc
     5941 gccggcggcc tccagggttt cggtcgcgag caggctgatc gcgcggctgt tgccgatcac
     6001 cgcgaggcgg ttgttgtaga ccggcttggc ggtgccgagc gcctcgaccg agttgaacag
     6061 ctgctcgatc gtgcgcacgc gcaggatgcc ggcgcgccgg aacgccgagt cgtagacggc
     6121 gtcctcgttc ggattggcgc ggtagcgccc ggggcgcagc acgatggtcg gcttgatgcg
     6181 cgccgcggcg cgggcggccg acatgaactt gcgcgcgtcg ttgatgcttt ccagatacag
     6241 caggatcgcc ttgacgttgc cgtcgcgcgc caggtaatcg agcatgtcgc cgaaatcgac
     6301 gtcgatcttc gcgccgagtg acagcacgtg cgagaagccg atgttgtggc cgtgcgccca
     6361 gtcgagcacc gagcgcgtga tcgcgcagga ctgcgagatc agcgccaggc tgccgggcag
     6421 gatcggcgtg tggccgcgcg tgacgttgag gccgccgcgg gtcgcggcca gcccgaagga
     6481 gtccggaccg gcgatgcgca gcacgtgcgg cttggccgca tcgagcagcg cctggcggat
     6541 cgccgtgccg ccgtcgtccc agctcttcag catctcctcg ttcagcagca gcacggcgcg
     6601 tgtgccgagt tcgccgagcg tgcggatctg ctcgggcgcg ccggccatcg gcgtcgtgat
     6661 cacggcgaga tccggcgcga gcggcaggct ggccagatcg cggtagcaga ggatgccgcg
     6721 cagcgccttg cggtccgggt tcaccggcat gatcggcccg ttgaaaccct gacggatcag
     6781 gttctgcacg agctgcgcat cgggcgcatc gtccttgctg ccgcgaccga tcacggcgac
     6841 ggaacggggc ttgaatacgg cgtcgaggtt gcggatcgtc atcgcgggat tccggagcgg
     6901 caggaaacgg acagggagcg gatcgctgcg cagcgccacc cggtcgggcc ggggggcggg
     6961 cagcggtggc cggccagtgt agtcgagccg tttgccgggc cggttgacag gtatcaacat
     7021 gcacccgccc ggccggcggc atgcggcggg gccacgccgg gggcttgcgg gctgcatggc
     7081 gtgaaggccg ccttcccatt ttcgatgctg ccggtggcgc agccgcaagg tgcgtcaccg
     7141 gtcgaggatt tcgagcgtga acgatgaaac ccgaaacccc cggacggtcc tgatcgagaa
     7201 gccggccgac cggcactgga atctgcccga tcacgcccgg gcgcgcgcgc actttgactg
     7261 ggcagaggtg cgcgccggcc tcgccggcct gcccggaggc gggctcaaca tcggctggga
     7321 ggcgctggac cggcacgtgg ccgacggaaa cggcgcgcgt ctggcgctgc gctggctcgg
     7381 caaggacggc gccgtgcgcg actacaccta tgcgcagctg ctcgccgagg cgcagcgctt
     7441 cgcctgcgtg ctggagcggc tcggcgtcgg gccgggcgag tgtgtcgcgg tgctggccgg
     7501 gcgcattccg gagctctaca tcgccgcgct cggggcgctg cgtgccggca tcgtgctctg
     7561 cccgctgttc tcggccttcg ggccggagcc ggtgcatacc cggctcgaaa agagccacgc
     7621 gcgcgtgctg gtgacgacgg tgacgctgta ccagcgcaag atcgcggcgc tgcgcgacac
     7681 gctgcccgcg ctcgacaccg tgctgaccgt cggcggcgac tgccccggca ccttccgctt
     7741 cgaggcgctg atggcgcagg ccgaaaccgg ctgcccggtc gccgcgaccg gtcccgacag
     7801 cccggcgctg ctgcattaca ccagcggcac gaccggttcg ccgaagggcg cgctgcacgt
     7861 gcacggtgca gtggccgccc atgcgtggag cggccgggcc gtgctcgacc tgcgccccgg
     7921 cgacgtctac tggtgcacgg ccgaccccgg ctgggtcacc ggcatctcgt acggcctgat
     7981 cgcgccgctg gtcaacggcg tgacgatgat catcgacgag gccgaattcg acgcgcagcg
     8041 ctggtatcgc atcctcgacg agcagcaggt cagcatctgg tacaccgcgc cgaccgcgat
     8101 ccgcatgctg atgaagctcg gcgacgcgcc gatggccggg cgcacgctgc cggcgctgcg
     8161 gctgatggcg agcgtcggcg aaccgctgaa ccccgaggcc gtgctgtggg gccagcgcgt
     8221 gttcggccag cccttccacg acacctggtg gcagaccgag accggcgcga tcatggtggc
     8281 caactacggc agcgagccgg tggctccggg ctcgatgggc cggccgctgc cgggcatcga
     8341 cgccgcgatc gtgcaccgcc ggcgcgacgg cggcctcgat ttcctgcccg atggcgaggc
     8401 cggcgaactg gcgatccgcg ccggctggcc gtcgatgttc cgcgcctatc tcgacgagcc
     8461 cgagcgttat gccagctgct tcgcagacgg ctggtatctg tccggggacc gcgcgcagcg
     8521 cgatgccgac ggccgctact ggttcgtcgg ccgcgccgat gacgtgatca agacttcagg
     8581 ccacctgatc gggccgttcg aggtcgaaag cgtgctgctc gaacatccgg ccgtcgccga
     8641 ggccggcgtc atcggcaagc ccgatccgtg ggccggcgag ctgatcaagg ccttcgtggc
     8701 attgaagccc ggccataccg gtgacgatgc gctgcgcaag tcgctgatcg cacatgcccg
     8761 cacccggctc gggccggcgg tggcgccgcg cgagctcggc ttcgtcgaca gcctgccgaa
     8821 gacgcgcagc ggcaagatcc tgcgccggct gctgaaggcg cgcgaactcg ggctgcccga
     8881 gggcgacctg tcgacgctcg aaggtcacga gtcttgacaa aaaatcgcac ttccgggcgg
     8941 ccggaactgg tatgaccaat gtttgcacca gattcgtgcg aatgatttgt tttataaaca
     9001 atggcttgca cgatatggtg caagcaggcg cggcagatat tgcgttgcag cataaccggg
     9061 ttccggggtt ataatgtgaa gcagaattaa tcaaacctgc ttcttgcgct tccccccgag
     9121 gtataccgcc atgaccgtcg aacagatccg agccaccatc ctgcgcgtcc tcagcgaggt
     9181 cgccccgaac gccgacgtca gcgacgtcaa tccgaacgtc agcttccatg accagttcga
     9241 gatcgactcg atcgacttcc tgcggctgat gaccagcctc gaaaaggaac tgcaggtcac
     9301 gatcctcgac ttcgactacc cgaagctgtc gacgctgcag ggctgcgagg actacctgcg
     9361 gccgcagctc gcggcctgag tcgggccggc gatgccttcc ggcaccggcg gtccgcgagc
     9421 cgccggtgcc gctgcggcct caggcctggc cgatcagcag cagaccctgc acgaggccgt
     9481 agccgaccgt cagcgcgatg ccgaggctga tcagcccgat caggctgccg gcgatcacca
     9541 gcacgccgtc gcgttccagc atgccgagcg cgatcagcac gatgcagagc gccggcggaa
     9601 agttggcgag cgggatcggc agcgccagca cgatggcctg gaccgcacac cagccgccga
     9661 gcagccgttc ggcgcagcgc ccggtgcatt gcgtcagtcg cggctgcagc cagcgttcga
     9721 tgcggcgcag ccacggcagc gtgcgtgcca gcatcgaatc gaagctgctg cgcgggatcg
     9781 acttcttcag cagccagccc ggcagccacg gcagcgagcg gccgtacatc atctgcgcgc
     9841 agagcagcag cagcggcagg ccgaacaccg tcgacgagcc cggcggcagc ggcaccaggt
     9901 tcggcagggc aaacagcagc agcagcagcc cgaacgagcg cttgccgagc agcgcgacca
     9961 gcgtgccgag cgcgacgcgc tcgccgcgca ggtgtacggc atagccttcg agcagttcgg
    10021 aggtgcggac ccggtcggtg aagaccctgg cgaacgatgt catgcgtctg gttcccggtg
    10081 gccggaggaa tgcgcgattc aggactgacc gcagccggag gccggcgtgc ggaagcaggg
    10141 cgatcggagc gtggctgcgt gcatcggcac ggcattcgat ccgaaatctt gtaccgtaat
    10201 ggcatgacta atgcattaat ttttcgggac agccctggag ttctgccatg cagtcgctgc
    10261 gcgtcatcct cgtcgatccc agttccaccc gttccgccat tctggagcag gccctgcgcg
    10321 atgccggcct gcaggtgatc ggccgcttcg ccgagtgcga catgctggtc gaccggatct
    10381 ccgaactgca gcccgacatc gtgctggtcg acatcgaatc gccggaccgc gacacgctgg
    10441 agagcctgca gacggtcggg cgtgacaacc cgcggccgat cgtcgtgttc gccgaggatg
    10501 actccccgga tgccatcgcg gccgccgtgc gcgccggggt cagtgcctac gtcgtcgacg
    10561 gtctgcagcc gcaccgcgtg cggccgatcg tcgaggtcgc gatcgcgcgc ttccgggagt
    10621 tccaggcgct gcgccgcgaa ctcgaagcca cgcgcagcca gctcgccgag cgcaagctga
    10681 tcgacaaggc caagggcctg ctgatggaac accgcaacca gaccgaggcc gaagcctatg
    10741 cgtcgctgcg caagctcgcg atggaccgca accagcgcat cgccgaggtc gcacgcaacg
    10801 tcatcgatgt gttcgaactg ctgggctgag cggctgcgcc cggcactgcg cgcatggcgg
    10861 cgcggtgtct ccacggaatc tccacggatg acggaaacag gcaggaagct tcagggatcg
    10921 atcatgaaaa atcgtttacc ggactgattt ccggaaactt ctgcatttgg ccggtcgtcg
    10981 gctggctatg ttggcggggt gggggaagaa gggggaatgc cacattctgc ctgtgggaga
    11041 ccttcgccat ggccgtcagt ctgcgactgc ttgaaaccgc cgatgcgccg cgcatccagc
    11101 atctcgtctc cgatccgcgc ctggcctcct gggccaacct gccgcggccg tatccggccg
    11161 atggcgccga gcgcttcctg cagctcgccg actgccagca gcgccgtggc accggccggc
    11221 acttcgcgat cgagcgcttc gggctgctgg tcggcgtgat ctcggtcggc tgcggtaccg
    11281 atccggggct ggcgatgctc ggctactgga tcggggtgcc gtggcaggga ctcggcgttg
    11341 ccagccgtgc gctcgatcaa ctgctgaatc tcgatccgct ggccggaccg gggctgcgca
    11401 cctggggatc ggtctgtctg gagcgcaacc tcgccagtcg ccgcgtgctc gagaagaacg
    11461 gcttcaccgc cgtcgccgcc cgtccctacg aaggcccgta cccgcaccgc ttcgccggcc
    11521 agacctgcct gacctacctg cgcatgccga acccgaacgc ggtgctgatc gcctgaaccg
    11581 gcagccgcca tgccggcagc catggcagat cccacccggg ccggaactga cccggatcat
    11641 ggacaggcag gtccggattg tccggaatcg gcaggcatgc agcggctggt caggccggtg
    11701 ctgcccttgc gctgcctact gccggattct cgatggaacc gccgctgccg ccacagtccg
    11761 ccaccgtgcc ggccgacgat gatctgccgc cgctgctgct gctcgtcgat gacgacgccc
    11821 ttgcccgcct ggcgctcgaa gccatgctcg ccggggacgg ctaccggctg gaaaccgccg
    11881 cggatggcag cgaggcgctg gtgctcgccg cgcgcctgct gccggatctg atcctgctcg
    11941 acgtgctgat gccgggaatg gacggctacg aggtctgccg gcgcctgcgc gccgatccgg
    12001 cgctcgccga ggtgccggtg ctgatgctga ccgcgctcga cgatgccgcc tcgcgcctgc
    12061 tcgggctcga cgccggtgcc gacgacttca tcgccaaacc cgccgaccgc gccgagctgc
    12121 gcgcgcgcgt gcgctcgatc acgcggctga accgctaccg ccgtgcccgc gagcgcgagc
    12181 gccggctcgc cgagagccag cgtcagcagc tgcattcgct ggcgctgctg cagtgcgtga
    12241 tcgacaactc gccggatgcc ttcctcgcgc tcgacgagaa cggcgagatc gtcgaatgga
    12301 gcgcgcaggc cgagaccatg ttcggctggt cgcgcgccga ggtgctgggg ctggacctga
    12361 acgatctgat cgtgccgtcg cacctgcgcg tgcagcagcg ccgcctgcac gaggcggtgc
    12421 gccagaccgg ccgcttcgac ggtttcggac gggtcgagga gcgcgtgtcc gtgcggcgcg
    12481 acggcagcga gttcccctgc gaggtgctgc tgacggcgct gccgaccgat cgcggctggc
    12541 ggctggccgg cttcgtgcgc gacatcggcg agcggcgcga tgccgagatc cggctgcgcc
    12601 aggccgagaa gctcgaagcc atcggcctgc tgaccggcgg actcgcgcac gacttcaaca
    12661 acctgctgaa catgatcctc ggcgggctcg aactgctggc cccccggctg ccggccgggg
    12721 atgcgcgggc gctgcgtcac cacgatctgg ccctgcgcgc tgcgcgtcgc gccgccgagg
    12781 tcacgcgggc gctgctgcag gtcgcgcgcc gccagccgct cgatgcgcgc gaggtcgacg
    12841 tggccgcgca ctgcatcgag ctgctgccgc tgatccgcca gtcggccggc tcgggcgtgc
    12901 gcatcgtgct gcatggcttc gaacccgcgc cgcagccgcc gctgcgggcc tgcgtcgatg
    12961 ccggcggcct cggcaacgca ttgctgaacc tcgtgatcaa cgcccgcgat gcgatgccgg
    13021 gcggcggcac gctgaccctg gaggtgcagc tgcagaccct gcccgccggc gccgtgcccg
    13081 aactggcggc cggcacctgg atcgtgatcg ccgtcaccga taccggctgc ggcatgtcgc
    13141 cggcggtgcg ggcgcgggcc ttcgatccgt tcttcacgac caagcaccgc ggccagggca
    13201 ccgggctcgg gctggcgatg gtgctcggtt acgcccgcca gtccggcggc aatgtcacgc
    13261 tcgccggcgc tgcgggcggc ggcacgacgg tccggctgta tctgccggcc gtggcaccga
    13321 tgagtccgcg cccggaggca gcgctgccgg tgccgggggt ggccgccccg gccggccagg
    13381 gcgagcgcgt gctgctggtc gatgatgaat ccggcctgct cgaactcggg cgcgaatggc
    13441 tggaggccga tggctaccgg gtcgatacct gcagcgatcc gcagcaggcc ctggcacggc
    13501 tgcaggccgg cgggcacgcg ctgctcgtca ccgacgtgac gatgccgggt ccgctcgatg
    13561 gctgcgcact ggccgcgcat gcctgccggc agcagcccgg gctgcgggtg ctgctgatct
    13621 cgggctacgc cgatgacgac gcgctggccg agctgtcgcg ctggccgctg ctgctcaagc
    13681 cgttcactgc ggtcgaactg ctgcaggcgg tgcggcgcag gctggatcag cccgggggcg
    13741 gctgaggccg ggattcgtgc atgaagattt cgtgacagcc gccgaacccg ctccgggata
    13801 cggggcgggg aatcccgatc gccaacagga tggaaaaccg atgaaccctg accacgcccg
    13861 ccgcccgcgc ctgctggtcg tcgatgacga agccgacaac cgcgagttct tcgccgagac
    13921 cggcgatctc gccggctacg cggtcgcaag cctcggcgat gtccgcgagc tggaatccgc
    13981 gctgcagccg gcgccggacg tgatcctgct cgacctgatc atgccgcacc tcgacggcat
    14041 cgaggtcatc cgcgaactcg cacgtgccgg ctgtcgctcc aggctgatcc tggtcagcgg
    14101 ctgcgagcgc agggtgctgc aggcggcgct gcggctcgcg caggggttcg gactcgatgt
    14161 gctcggcgaa ctgcagaagc cggtgtccgt cgccgcgctg accgcgctgc tgctgcagcc
    14221 gggcatgccg ccggcccggg tgcagccgct gcagccggtg acgctggacg aactgcacgc
    14281 ggcgctcgcc gagaaccgct tcaccgtgca ttaccagccg caggtggcgc tgcacgacgg
    14341 gcgctggtgc ggggtcgagg gactggtgcg ctggtgccac ccgcagcacg gcctgctgct
    14401 gcccgacagc ttcgtgccgc tcgtcgaacg gggcggactc gggctggaac tcacccgctg
    14461 cgtgctcgaa tgcgccctcg ccgatcttgc gcacctgcac ggcctgctgc cgggcggccg
    14521 cctgccggga ctgtcggtca acctgccggg cgaggccttg ttcgacgtgt cgctgcccga
    14581 gcagctgctc gcgctggtcg atgcggcggc ctggcagcac gacgcgatca ccttcgagat
    14641 caccgagacc tcgctggccc gcgagccggt ccgggccctc gacatcctga cgcgcctgcg
    14701 gctgaagggc gtgcagctgt cgatcgacga cttcggcacc ggttactcgt cgttcgggca
    14761 gctgcatcac ctgccgttct cggagctgaa gatcgatctc ggcttcgtgc gcgtcgccga
    14821 tcgcgatccg gtggcgcggg cgatcgtgga aaactcgatc cggctcgggc gccagctcgg
    14881 gctgaccgtg gtcgccgaag gcgtcgagag cgcgacgctg tggcactggc tgcgcgagca
    14941 gggctgcgat ctcgcgcagg gctacctggt ctcgcggccg atgccggccg agcagctgcc
    15001 ggcctggtac gcggcctggg gggcaccggc ctgtccggcc tgatcagcgg cggcgcaaca
    15061 agccgatttt gttgttgtaa atacaaaacg gcttttcttt cttgcaacaa tgccgttgcc
    15121 tgctacaaca gggaccgtcc gcatgcgtcg gcaagaacgc ggacggccga agcctcgccg
    15181 tgcgtcagcc cctgcacgtc ccgcgcttcc tgcaatcttg catttgttgt gcattcatcc
    15241 gcatttgccc tgcatctgcc ggattgctgc acgcgcaagt cgggaacagg atcggccgat
    15301 atccccgtca gcagcgcccg ccggctggtc cgtcgcagcg tctgcatgcc ggttcgtggc
    15361 ggccgaacga gcgccaccgg ccggtgtccg ttcttacgga gtgacccctg gaggcttgca
    15421 cgatggatgc tcagccgatg ttccccgccg acatgcccga tgcggtctgc gcctgttccc
    15481 gcccgttcct gcaggggcac gccagcgctg ccgagccggt gttcccggtc gatcgcgacc
    15541 tcgaacacct gtacgtgcag gccgcgcatc gcgcccgcct gctcggtcag gcgcagttcg
    15601 aggcgatcgc gcgtcatgcg catcccgatc cgctgcgcag ccaccggcat gcggactgag
    15661 ccggctgccg gcagcggggc gcggggcgtc agtccgcggg caggcagcgg ccggtccgca
    15721 gccagtggtt cagccgtcgc tcccagtcgc gcatgaacac caggtagtgc tccgcggtat
    15781 aggccgcgac cgcagcatcg ccggcaacgc tgccgaggct ggtcagccgg tagcggaccg
    15841 tcgcgagaca ggccgaatcg ccatcggcct gcagccagat ctccagccgg caggccgtgt
    15901 gctccggcac gaagcgcacc atctccacgt aaccctgcac tgcatcgtgg cgggtcacgt
    15961 accacagcgc gctgcccccg gccggggccg gtgtcgcgaa cacgcagtcg ggctcggcga
    16021 ggcccgaggc actgatcacg tactgcggac gccagtccgc gatccagtcc gtctcccgta
    16081 ccggacagag cagcggcagc acctgcgccg gtgtggcgca gagccgctgc acgtagctgt
    16141 gctcggcgcg caccggcgcg cgcaggggca cggcatagcc ggcccggccg ctccagagcg
    16201 ggtcgagttc gtgcgccagg cacggcggcg aggcgcagtc gggggtgtcg gggcgcggtt
    16261 cggtcatggg ctggcacctg gctggcacgg ccggttcagg ctgcggtggc agcctgcggc
    16321 aggccggcaa tgacgatttc gagcacgcgt gcggcgtcgg cggcatcgaa gtctgcaccg
    16381 tggcgcttgc gcacgccgtg atcggacagc agatgatagg tgccggcgct gacgccggcc
    16441 cgggcgaggc aatggcgcac gcaggcgagc gcgcagccat cgagcgcgat gaccggtcgc
    16501 gtgccctgcg cgacgcgcac cagcgccggc acgccgccgc cgactccggc gatgcacgac
    16561 atctcggcga gcccgcggcg atcgagcgtc agcgcgacgt ggttggcgag ttgcgcagca
    16621 ctcgaacagc ccgagcagct gtagaccagg ggcagggtgc ggtcggtacg ggacatcggt
    16681 aacgcctcat cgtgatgtga aatatccgca aggctatttt ttgcatcgcg tcccgccctt
    16741 gagatgcatc aagccggcac cgctgcctgt ccgcccccgg cacccgcttc acccgccgga
    16801 tgcgcccggc cggccgcgga cggggcggtc aatcgtcaca agttgcggga atccggccgg
    16861 attcccgggg ttcgccttgt ccgtcgtccg gtcacgtggc accttcccgc gcctgaaata
    16921 ccggccggtc cgcagccggc gctgccggca gcggcagcgc ggccccaggc cggaggaacc
    16981 atccggcgct gcgcaccagg cgcagcgccg gattcatacc cgcacgggca ggacgggctg
    17041 taccgaccat gagtgaactt tcccagaccc ccgccggtgc ccggcgtctg ccgctggcgc
    17101 gctacctgtg gcagtcctac ctgcgcgccg cgatcatccc gctgctggtg atcgaactcg
    17161 gcttcatcgg cgtgtactgg gtgtcgagct tcatcaccta cgagcgcaac gccgaggcac
    17221 tcggggcgat ctcgcgcgag gatctcgccc gcaccgcgca gcgcgaggcc gatgcgatcg
    17281 ccgaaaagct cggcgcggtc ggggacctga cgcgcgtgtt cgcggccgaa accgcgcgtg
    17341 ccctgcgcac gccggccccg gcggcggcga ccgatgacga tgcccgccac gcctacggcg
    17401 cggatggcgt ctactacacg acgcgcgagg gcgtctcggc cggcttctac agcggcatcg
    17461 tgccggtcgg cccggccgag cgcgacaagg tgcggcgcac ggcggcgatc gatccgctga
    17521 tgcaggcgat caaggcggcc aacccgctgg tcgcgcagct ctacctgaac acgcacgaca
    17581 gctacaaccg catccatccg tacttcgagg tgctgacgca gtacccggcg aagatggaca
    17641 tccccagtta caacttctac tacgaggccg acgccgcgca cgaccccgaa cggcgcgcgg
    17701 tctggaccga cgcctacgtc gacccggccg gcagcggctg gatggtgtcg tcgatcgcgc
    17761 cggtctacgg cggcgccgac ggcagcttcc tcgaaggcgt ggtcggcatc gacgtgaccg
    17821 tcgacaccat cgtcaggcgc gtgctgaacc tgcagctgcc ctggcagggc tacgcgctgc
    17881 tggtcgggcg cgacggcacg atcctggcgc tgccgccgcg cggcgaacgg gatttcggcc
    17941 tgaaggagct gaccgcgcac agctacgacg aggcgatccg cgccgacacc ttcaagcccg
    18001 atgacttcaa cgtccacaag cgcgccgacc tcgccgtgct cggccgggcg ctgaaggccg
    18061 gcggggacgg tgtcgtcgaa ctcgcgctcg gcagcggccg cacgctggcc ggctggtcga
    18121 cggtggccgg gcccggctgg cagctggtgg tgctcgcacc ggagcacgag atcctcgccg
    18181 gggccacggc gctgcgcgag cggctgtcgc tggtcggctg gctgatgatc gcgagcctga
    18241 tgctgttcta cgtcggcttc ctgatctacc tgcgcagccg cgcccgggcg atgaccgcgc
    18301 gcgtggccgc gccgctcggc gacatcgagg cgctgatccg gcgcatcggc ggcggcgact
    18361 acgagcagtc cgagcccgac taccgcatcg ccgagctgca gaccgtcggc acgcacctgg
    18421 tcgacatggg ccggcgtctc ggtgccgcct acgcggcgat ggtcgaggcc gaacgcagcg
    18481 tgcgcgccgc gctcgacaag gagcgcgaac tcggcgaggt gcagcgtcag ttcatcaacg
    18541 tcgtctcgca cgagttccgc acgccgctga ccatcatcga cggcgtcgcg cagaccttcg
    18601 agcggcgtgc cgaccgcctc ggccccgaca agatcatcga acgcgcgcgc agcctgcgcg
    18661 cctcggtgcg ccggctggtc gacgtgctcg aaagcgcgct gtcgttctcg cgcctggaga
    18721 gcagccagct cacgcatgcc ttcgggccgg tggccttcgc ggccgtgctg caggaggtcg
    18781 tgcagaccga gaccgtcgcc tatccgcagc accgcttcga gatcgacggc gcgctgccgg
    18841 cgacggtgca cggcgatgcc gggctgctgc gcgtcatgct gtcggccgtg ctcgacaacg
    18901 ccgcgcgcta ctcgccgcaa gcgggcacgg tcacgatccg cgccagcacc ggcaccgatg
    18961 actgcgtgca tctcgacatc cacgaccagg gaccgggcat tccggccgat gagctgccgc
    19021 gcgtgcgcga gcgtttctac cgcggcagtg ccgggcttgc gagtcacggg gccgggctcg
    19081 gcctgtatct ggtcgagcag ttcgtgcagc tgagcggcgg gcggctggac atcgacagcc
    19141 cgccggggca gggcacggtc gtgcgtctgg tgctgccggt gcatggtccg cgcgccgcgg
    19201 cagcggcctg aacggaggag ggcccgtcat ggacacgaac ggtgcagtga tcctgtgcgt
    19261 cgaggatgaa gccgaaatcc gccgcgatct cgtcgacgaa ctgctcgatg ccggctacga
    19321 ggtgcacgag gccggcagcg gcagcgaggc gatcgggcgg ctcgccggcc tgcagcccga
    19381 cctgatcctc tgcgatgtca acatgcccgg cctgagcggc ctgcagctgc tcgaacaggt
    19441 gcgtgcgcgc ggcgatgcgc tcggcgaggt gccgttcgtg ctgctgaccg cctacggcga
    19501 ccgcgaacac cagatccgcg gccgcgcgct cggggccgat gactacctga tcaagccggt
    19561 cgactacgac ctgctgctcg ccacgctcgc gagccgcctg cgcaacgccc ggcgcgtgca
    19621 tgcgcgcgtg cagtcgcgca tcggcgatct cgaatcgcag ctggccggcg tggagcgcag
    19681 gacggccacg ctgccggacc ggatggcctt gcaggcagcc cttgccgacg ggcgcggccg
    19741 gcgccggcgc ggcctgctgg ttgcgtcctt cgatgagctc agccgccacg tccagcaatt
    19801 cggcagcagc ttccaggacg aggccgagca ggcgctgatc gggcaggtgc agctgctggc
    19861 tccggcgcag gcctaccgcc tcgattccga gaccttcggc ctgctgttcg ccgatgccga
    19921 tgcgctcgat gtgtcggcac tcgggcggct ggtcgagatc gaatgccgca gcccgggggg
    19981 cgccgcgccg gtgcgcctga gcacgacgct ctgctacgtg cactgcgagc ccgaggccgg
    20041 ctgcgacgtg gccgaccggc tggaggatgc gtgcctcgcc gtgcgcttcg gccggcgcga
    20101 cggcggccat acgctgctgc gcctcgacgc ggcggccgtg cagcggctgc gcatggccgg
    20161 ccacgtcgag gatcacctgc tgcgggcgct gcgcgacggc gagttcacgc tgcacttcca
    20221 gcccaagctg cagctcgccg atgatcgtct ggtcggtgcc gaggcgctgg tgcgctggca
    20281 cagccgggat ctcggaccga tctcgcccgg cgtgttcatc ccgatcgccg agcgctgcaa
    20341 cctgatcgag gcgctgagcg tctgggtgct cgacgaggcc gcgcgcgccg cggcggtgct
    20401 cgcgcgcgag gggctggatc ttgcgatcgc cgtcaatgcc tcgggtgtcg aactgcacgc
    20461 cggcttcccg gcgcgcgtgc gcgaggtcat ggccgcgcat ggtctcggtg ccggccagct
    20521 cgacatcgag ctgaccgaaa cctcggtgat cgccgacatc cgccacgccg ccggcatcgt
    20581 cgccgagctg cgcgggctcg gcgtcggcgt cgcgatcgac gatttcggca ccggctacgg
    20641 ctcgctttcg tacctgcgcg cgctgccggt cgataccgtc aagatcgacc agagcttcgt
    20701 gcgcggcctg cccggcaacg ccatcgaccg tcagatcatc gagagcgtgc tgacgctcgc
    20761 gcacgcgctc ggcatcggca cggtcgccga aggcgtcgag accgccgagc aggagcgcga
    20821 gatgcgccgc ctcggctgcg gccagatcca gggctatcac ctggcgcggc cgatgccgct
    20881 caccgaactg atccgcttcg tgcgcgagcg gcgctgagcc cgccacagac aagaaacggg
    20941 acgggacgac gatgacaccg gccggcacga cgctgctcgt ggtcgaggac gagcaggagc
    21001 tgcgcgagta cctggtcgag gaactcgaag acctcggcta tcaggtctgc gaggccggca
    21061 acggcgcgct ggccctggcg ctgctgcgcg agcggccggt cgatctggtg ctgaccgaca
    21121 tcatgatgcc gcagctcggc ggtgccgggc tctaccacgc cacgcgctgc gatccggcac
    21181 tcgcgcagct gccgttcctg ttcctgaccg cgctgtcctc ggccgagatg ctggcgacgc
    21241 tgccggagag cgagcagccg cacatcctga agaagccggt cgactacgac gagcttgccg
    21301 acgcgatccg ggcgcggctg gcgtagccag acggacgccg ctagccggcg tgcgggcgtt
    21361 cgagcacgac cgcgaccccc atgccgccgg ccgtgcagac ggcaagcagc gcccgccggc
    21421 cgccaccggc cgcgagccgg cgggcggcgc tcagcagcac gcgggcaccg gtggccgcga
    21481 acgggtggcc gagcgcgagg ctggagccgc aggcgttgag ccgcgccgga tcgggctcgc
    21541 cgagcgccgc ctccagcccg agccgcgcgc ggcaccagtc ggccgagcgc cacgccgcca
    21601 gcgtgcagag cacctgcgcg gcgaaggcct cgtgcagctc gatcacgtcg aagtccgcga
    21661 acccgagccc gaggcgtttg agcagccggc cgaccgcgag cgccggtgcg agcagcaggc
    21721 cctcgccggc gacgaaatcg acggccgccg actgctgcgc gacccagtgc gcgagcaccg
    21781 gcaggccgcg ccgggccgcg gtccgggcct cgcagagcag cacggccgcg gcgccgtcgg
    21841 tcagcgccga ggcattgccg gcggtcagcg tgccgtcggg ggcgaaggcc gggcgcagcc
    21901 gggcgagctg ctcggcatcg ctgccggcgc gcggcaggcg gtcggagacc acgccggcgc
    21961 agggcacgag ttcggcgtcg aaccagccgg cggcgcgcgc cgccagcgcc ttgcggtggc
    22021 tgtcggcagc gaaggcatcc tgttcggcgc gggtgatgcc ccattcgcgc gccatgcgct
    22081 cggtgtgctg acccatcgac agcccggtgc gcggctcggc cgggctcggt gccaccggca
    22141 gcagctcgcg cagcctgaag ccgcgccact gcgccagccg ctcccgcagg ctgcgcgcgc
    22201 gctgcgtccg gatcaggcgc tgcgcgaaac gccggtcgaa cacgatcggc aggtccgagg
    22261 ccgtgtcgct gccggcggcg atgccgctgt cgatctcgcc ggtggcgatc ttcgcggcca
    22321 gcacgccggc cgcctgcagg ctggtcgcgc aggcctgcgt cagcgtcacc gccggcgtgt
    22381 ccggcgcgag cccggcggac agcacggcct cgcgtgccag gttccagtcc ttcgcgtgcg
    22441 agttgacggc gccggcccag acctcgtcga tgccgtcgcg gcgcaggtcg tgacgcttgc
    22501 ccagcgcgcc gagcaccgcc gacagcaggt cgaggttcga aagctccgcg tattcggcac
    22561 cggcgcggca gaacggcgtg cgcaggccgt cgacgacggc gacccggcgc ggggcgaagt
    22621 cactcatccg cggtctcccc gtcgatgcag gtgcaggcgg gggcgttcat gccagccccc
    22681 ggtagcgcag cggtccgccg cgaaacggcg cgaagccggt gccgaggatg acgccggcat
    22741 caagctgctc ggcgctggcg acgacgccgt cggcgagcgc gcgttcgcag gcatcgagca
    22801 gcggtgccag catcgcatcg gcgagcgccg caagttcggc cggcgtgccg gtggcggccg
    22861 ccgggcgccg gaccttgccg tccttccaga cgtaaaagcc ctgcccggac ttgcgcccga
    22921 gccggccggc ggcgatgcgg gcatcgaggg ctgcggcggc ggccggttcg gcggggccga
    22981 gcgtgaccag cacgtgccgg cagacatcga gcccgaccgt gtcggcgagt tcgatcgggc
    23041 ccaccggcat gccgaagccg acggccgcgg catcgagcgt cgaggccggc gtgccggcga
    23101 gcagcaggcg cagtgccgcg agcaggtagg gtgcgagcac gcgattgacc agaaagcccg
    23161 gctggctgcg gcacggcagc gggtacttgc cgagctgcac cgccagtgcg cagccgcgtg
    23221 cgaccgcggc cggcgtgctg gcctcgccgt gcacgacctc gaccagcggc atgcgcggca
    23281 ccggattgaa gaagtgcagg ccgatcagcc gcccgggatc ggtgagggcc gaagccagcg
    23341 tttcgagcgg cagcgccgag gtgttggtcg cgagcagcgc atgcgccggc agccgcgctt
    23401 cgagcgcggc gaacagcgtg cgcttcacgc cggcatcctc gatgacggcc tcgatcagca
    23461 cgtcggcgcg cgcgacgccg tcgccggccg gatcggcgat cagccggccg gcggcggcga
    23521 ggcgttcggc cggcgtcttc agccggcgcg cgaacagccc gcgggcgcgt gcgagcgcgg
    23581 cgtcgatgcg cgcggcatcg aggtcgtgca gcgtgacttc gagcccggcc agtgccgcaa
    23641 ccgcggcgat gtcgccgccc atgacgccgg cgccctcgac gtgcaggcgg tgcgccaccg
    23701 gggcggcatt ctggcgcccg aggctcttca gctgctcggt cgcgaagtac acgcggcgca
    23761 ggttgcgcga cacctcgccg gtcagcagcc cggcgacctc gcgggcctcg ccttcgagca
    23821 tgcgtgccgc atcgccgccg tcgagccgcc agtgctcgat cagccggaag ggcgccgggt
    23881 aatgatcggc gcgcaccttc tgcgccgtct gccggcggat caacggcgcg atcacggccc
    23941 gggccgcggc gctgttcgac agttgcgcga gccagcccgg ccggctgcgc cgcccggcga
    24001 gcacggcgcg ccgcgccagc cagtccggcg attcgtgcgt gccggcgacg gcatcgacca
    24061 gcccggcggc gctggcggcg cgcgccgcca gcgtgcggga agtcagcatc agcccgagtg
    24121 ccgccggtcc gccgagccgg cgcgtcgcgc gcagcgtgcc gccgaagccg ggatagatgc
    24181 cgaggcgcac ctccgggaaa ccgagccggg tcgagggcag gtcgtgcgcg acgcgccagt
    24241 ggcaggcgag cgcgagttcc agaccgccgc cgagacagtg gccgtggatc acgcaggcca
    24301 ccggccacgg cgcggcttcg aggcgcgcga acagcccgtg cacgtgccgg atcaggctgg
    24361 cgacctcgtc tgcatcgcga tagccgtcga actcgcgcag gtcggcgccg gccgcgaagc
    24421 ccgcgggctt gcccgagcgc agcacgaggc cgcgcggcgg ggcggcgtcg agcgcatcga
    24481 gcacctgccc gaattcttcg agcaccgcgc gcccgagcgt gttcggcccg tcaccggcgc
    24541 aatcgagcgt cagccaggcg atgccgtcgg gatcgtgcct cagctgccag tgctgccaga
    24601 cggcatcgct cgtcatgccc gtgccccggc gaaggccgcg gggcgcaggc cggccggttc
    24661 cggggccgag ggcgggattt cgggcccgga cggcggcggc gggacttcgg gcgtggccgg
    24721 cggttcgggc gcttcgggcg gcgacggttc ggccgggcgt tcgggtggcg tggactccgg
    24781 aatctcgggg ccggagggcg gcggcggaag ctcggggccg gtggcgacgg tcgcgacgcc
    24841 gtggccggcg agggctgccg ggtcgaagtc atcgacggcg atcacgcgcc gcaccagcgc
    24901 cgcggcctgt tcgagctgcg cgaactctcc ggccgtcagt tcgccggccg caagtgctgc
    24961 cgccgggtcc ggggcctgca gccggcccct gcgcttcgcg gcctgcagcc gggcttcgat
    25021 cgcttcggcg gcgaccacgg cctcgaaggc cagttccagc gcgccgaccg cctcggccgg
    25081 atcgcggctg acgtggatcg ggccgctcag gcgctcgcgc gccggtcccg gcgacatcat
    25141 cagttcggcg acgcgcgccg actgcgcatc gcgggcgccg cgcatgcgcc ggccccacgg
    25201 gaacacgagg cggcgcagca ggctgccgag cagcggccgc gggaagtttg cgagcgcatc
    25261 ggacaggcgc ttctcgatcg cgtagcaggc ttcggccagc acgtagtcga gacaggcgag
    25321 atcggcctcg gggcggccgt cgtcctcgta gcgcttcagc gccgccgagg ccaggtacag
    25381 ctcggcgagc gcatcggcga gccgcccgca caggcgctcg cggcgcttca gctcgccgcc
    25441 gagcgtgagc agcgccacgt cggcgaccag cgcgaacgcc gccgacaggc gcgacagctc
    25501 gcgataccag tggcgggtcg cggcgtcggc gccggcaggg gccggggcga aggcggcggc
    25561 gctcagcgcc cggccgccgg cgcgcgccag cgtgccgagc aggtgaccga ggtggccgcg
    25621 gaaggcctgg tcgaacgcag cgagcgccgc gcgccggtcg gggtttccgg cggccgccag
    25681 ttcgctgcgc agccacggat gacagcgcag ggccccctgg ccgaacacga tcagcgtgcg
    25741 cgtcaggatg ttcgcgccct cgacggtgat gccgatcggc agcgcctgat agacgccggc
    25801 cagccagttc gacggcccgt gacagatgcc cttgccgccg tggatgtcca tcgcgtcgtt
    25861 gaccgccgcg cgcatgcgct cggtcgactg gtacttgacg atcgccgaga ccacggccgg
    25921 cttctcgccg agatcgagtg ccagcgtgct cagccggcgt gcggcttcga gcacgtaggc
    25981 gtggccgccg atgcgcgcca gcgcctcggc aacgccttcg aagcggccga tcggcagctt
    26041 gaactgccgg cgcacgcgcg cgtaggcacc gctgacgcgc gctgcgtaca gcgctccggc
    26101 cgtgccgttg gccggcagcg agatgccgcg gccggccgcg aggcattcca tcagcatcgt
    26161 ccagccgtgc ccggcgcagg ccgggccgcc gatgatccag tcgagcggga tgaacacgtc
    26221 gcgcccgcgc gtcgggccgt tcatgaaggc ctggttgccg gggaagtgcc ggcggccgat
    26281 ctcgacgccg gggtgcgtgg tcgggatcag cgccagcgtg atgccgatgt cggcgcgatc
    26341 gccgagcagg ccgtcggggt cgtgcagccg gaaggcgagg ccgagcacgg tcgcgaccgg
    26401 tgccagcgtg atgtagcgct tgtcccagct cacgcggatg ccgagcgtct cgcggcccgc
    26461 gtgcaggccg cggcagacga cgccgcgatc cgggatcgac gcggcatcgg agccggcctc
    26521 ggggccggtc agcgcgaagc agggaatgtc ctcgccgcgc gcgagccgcg gcagccagtg
    26581 gtccttctgg gcctgcgtgc cgtaggcgag cagcagttcg gccggcccga gcgagttcgg
    26641 caccatgacc gtgaccgcga cgctgaccga gcgcgaggcg atgcgcgtga cgaccgccga
    26701 gtgcgcctgc gccgagaagc cgagcccgcc gtatgcgcgc ggcacgatca tgccgaggaa
    26761 gccctgcgtg cggatcgtct gccagagcgc cggcggcagg tcgcgatcga cgtgcgtgat
    26821 gcgccagtca tcgatctgcg cacagagcgc cgtggtcggg ccgtcgagga aggcctgttc
    26881 ttcggcactg agggccgggg ccggcagcga cagcagccgc tgccagtgcg gatcgccggc
    26941 gaacagttcc gcctcccacc agaccgtgcc ggcggcgagc gcctcgcgct cggtgtccga
    27001 catcggcggc tgcgcccggc gcacgtgatc gggcagcggc gcgagcaggc gcaggcgcag
    27061 cggcgtcagg ttccaggcga gcagcggcag ccaggccagc cagagcccgc gcacgccgtc
    27121 ggccagggca tcgaggagca gcaggccggc gccgaggacg gccgtggcca ccggcaggga
    27181 cacgcgggcg gcggccaggg ccaggccggc gagcagcagc aggatcagtc cggcgatggc
    27241 ggtgctcatg atcgggttct ccggcacggg ggcggatggc atgcgccggg caggaggggc
    27301 agccacgcct gtgctgacaa catccgtagc acagccgctg cccggaatca ggcgcttgcg
    27361 ctgcgctccg tcccggctgc acccggcttt ccagcggaag ccgcggccga tccggagggc
    27421 gacctgatga tggcagcccc tcgccgcagc gtcctgaaac gacctgtcac ggcgtgctgc
    27481 cgcatccggg gctggctgcg ccatgccggg gcagcaggac atcgggcagc ggtgaggggc
    27541 tgcgggttcc gtgtctcagc ggcgccggat ccagttcttc atggcttcca cgtcccgtgg
    27601 atcgtaggca tcgagccagt gttcggtctg ctgcaggcgc gactgcgcgg tcagcaggcc
    27661 gttctcgtcg ccggtgcgga tcaggcgctt gatgcgccgg cggtacagct gccagtcggc
    27721 gcagaggtct tcgagttcgg tctcgttggc cggcagcacg cccgagcgca tgcgcacccg
    27781 gtagccgatg cgctcgcgcc gctcggcatg cgcggcgagc acggctgcga gttcgccggg
    27841 cgccacgtcc acgtaggact ccaccgccgg gttgccgcag ccggcggcca gcaccgcgta
    27901 cagcagcacg accctcaggg cacgcatggc gagcggtccg gattcaggat cggcgctccg
    27961 catccatacc cgtggccgtg gccgtcaccg gctgcgggcg gcaggacggc atcgcgaggc
    28021 gcgagggctg tattccttgc aacgcagggc catgaggtgt atggcgtatc gttcatgatg
    28081 taaatttagt tgaacattaa atttcgactg atgatggttc aggaattgat tgacgctatg
    28141 cgaaattaaa atacaagttt attctgtatc acggcatctg tcggatgtcg cccccctcga
    28201 tctcggccgg cgctccccct gcgccggccg ctttttttcg ggctttcccc aacagcatgc
    28261 gggcagcacg aagacagcag gaagcatccg gcgctgcagt cgcgccaccc cctgcaccgg
    28321 ccggcggcag gaaagtgcat cgcagaccct gcgggatacg tcaattgccg gtggccggga
    28381 acgtctgctg gcctatgatc ggccgggtgc gcgatccgta tcggggccgg gtcgccgggg
    28441 ggcgaaatgg ttgcaagcga agcaggtgca ggctggtttc tcgaaggcgt cagcgatcag
    28501 ggcggccact ggctggtgcg catcaccggc acaccgttca tcgtcgggcg cgtcgagagc
    28561 tgcgacctgt acctgaatga caccggggtg tcgcgccagc atgcgcgact cgaattcgac
    28621 ggggccggcc ggccgctgct cagtgacgag ggcagcacca acggcacctt cgtcaactac
    28681 cagcgcctgg aggcccgcgc gccgcagtcg ctcaacgatg gcgacatcgt gcatttcgcc
    28741 aagatggcct tccggctggt gcatcgcgag cagtcgaagg ctccggcgcc ggtggtcgag
    28801 ggcatcgagg gcggcacgat gttccagcag gtggccgcgt tgccggaaac cttcacggct
    28861 gcggcccacg agaagctgtt cttcgatctg ctggcgaagg gcgcggtgtc gccgtggttc
    28921 cagccgatca tcagcctgcg ccacggcaag caggtcgttg cctacgaact gctcgggcgc
    28981 ggtgcgcagc cggggctgtc gagctcgccg gcggaactgc tgaagatcgc gcacgcgacc
    29041 ggccgcacgg tcgaactgag cgacctgttc cgcaagaccg gcatccgccg cgcgcaggag
    29101 ctggggctgt cgcgcgtgtt cttcaacacg atgccggccg agatgtcgat cccgctgctg
    29161 cgccggctgc tcggcgagct gcgcgtgatg gcgccggtgc tgccgatggt catggaggtc
    29221 cacgagcagg cgatcgccga catcgagctg atgcgcgatg tgcgcgcgct gctcggcgag
    29281 ctgaacatga agctcgccta cgacgacttc ggtgccggtc aggcgcggct ggtcgagatc
    29341 atggaagtgc cgcccgacgt gctgaagttc gacatcggcc tggtgcgcgg catcagctcc
    29401 aagccgcagc gcgtgcagac catggtcgcc gacctcgtgc gcatggccgc cggcatgggc
    29461 gtgcgcacgc tcgccgaggg catcgaggcg caggaggact ccgacttctg cgaggcggcc
    29521 ggcttcgacc tgatccaggg ctatctgtac ggccggccga cgccggaccc gctgcccgtc
    29581 gccggctgaa accgcgcccg ctctctttca gcgcgcaccg gcctgctcgc tgaagcgccc
    29641 gtcgcgcagg ctcagccgca cgctggccgg gcggcccggg aagttcggcg catcgtcggg
    29701 accgaccacc ttcgccggca ggctcagcac gatctcgccg ggcgtgcggc cacgggnnnn
    29761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccga ccaccttcgc cggcaggctc
    29881 agcacgatct cgccgggcgt gcggccacgg gcggcccgcg cctgcagttt ttcgatcgcc
    29941 cgccagccct tgccgccgat cgcgatcacg tcgagcagcg tgtagtacgg cttgccgccg
    30001 gcgccttcgc ccgcttcgaa ggctgcgagg tactgcgtat gacggttgcc gccgccgaaa
    30061 ccctcgaccg tgaacagcac cagcagcagc gtctgcccgg catcgggctg cagcgtctgc
    30121 gtttcggttg cctcgcgata ccagcttgcg tagccgtcac gcagcagctc gctcagccgc
    30181 tgcacctgtt cgagcagcgc cggcgggggt gcggcccaaa ccggcgggct caggcacagg
    30241 cccaggctca ggcaggcact ccgcagcaat acgatcgggc ggcgcatcgg caggaggact
    30301 ccagggctgg cggacggacg ccgccagcat aggcggccgg cgcaggacga cgggcttgcc
    30361 agccgcgggg cggcggtgtc agggtcaacg gaccggtctc cggcgagccg gcagcagccc
    30421 cggcccgtcg cggccggccc ggttccgttt gcctggagcc tgcgcgcatg cagagcaacc
    30481 tgattctcga caccgattcc tacaaggctt cgcactggct gcagtacccg ccgggcacga
    30541 cgcaggtcta cgcctacatc gaggcgcgtg cgagccacgt cgatggcgtc gacgcgacgc
    30601 tgttcttcgg cctgcaggcc ttcctgaaga cgtatctgtc gcggccggtc ggcgccgatg
    30661 acctcgccga agcgcgcgag ctgttcgcgg cgcacggcga gccgttcaac ctcgacggct
    30721 ggcagcacat cctcgatcgc cacggcggct ggctgccggt cgagatccgc gccgtgcccg
    30781 agggcagccg cgtgccgctg cgccaggcgc tggtgacgat cgagaacacc gatccggccg
    30841 tgccctggct gacgagctac ctcgaaaccg cgctgctgcg cgcgatctgg tatccgacca
    30901 cggtcgcgac catcagccgc acggccaagg aaaccctgcg cgccgcgctc gaacgcactt
    30961 ccgacgatcc ggccgcatcg ctggcgttca agctgcacga cttcggcgcc cgcggcgtgt
    31021 cctcggccga atcggcgcgt ctcggcggca tggcgcatct ggtcaacttc atgggcaccg
    31081 acacggtcga ggcgatccgc gcgatccgca cctgctacgg cccggccgac ggcatgccgg
    31141 cgttctcggt gccggccgcc gagcattcga cgatgacgat gtggggcgag gccggcgaac
    31201 tcgacgccta ccgcaacatg gtgcgccgct tcggccggcc cggcgcgatc tactcggtgg
    31261 tcagcgactc ctggagcctc gcgcgcgccg tcgacgagca ctggggccgg acgctgcgcg
    31321 acgaggtgct tgccgccggc ggcacgctgg tcgtgcggct cgattccggc gacccgatcg
    31381 acagcatccg ctacgcgctc gaatcgctcg ggcgcagctt cggtttcagc gtcaaccgca
    31441 agggttaccg cgtgctcgac ggcgtgcgcc tgctgcaggg cgacggcatc gatctttcga
    31501 gcctcgcgca tctctgcacg gccatcgccg atgccggctg gagcctcgac tgcttcggca
    31561 ccttcggcat gggtggggcg ctgctgcaga aggtcaaccg cgacaccttc tcgttcgcgc
    31621 agaaggcctc gtggggcgtt gtcgacggcg aggcgcgggc catctgcaag cgtccgcagg
    31681 gcgatccggg caaggcctcg aaggccgggc gcatcgccag ccaccgccac cgcggcgacg
    31741 gacgctggtg cacggcgatc gagggcacgc tcgatgccgg ggaatggcag ccgatgctgg
    31801 agccggtctg gcgcgacggc aagctgttgc gcgagcagga ttttgccgac gtgcgcgccc
    31861 gcgccgaagc cgggttcggc cgaagcgcgt ttccgtaggg agtatcgggg atcggcggcc
    31921 ggcgcgcgcc ggatgttcga ctatcctgac cggggtccgg tcgcctgacc cgtggccggc
    31981 atgcaccttc cctgtcggcc agacgtatcg aggagagcag gcatgaccga tatggacccc
    32041 gccatcgtcg agcgctttgt ctatgactgg tgtacggccg aagcgcgcgg cacgccgtcg
    32101 gcgcgcgcgc tcgctgcgat ctatgccagc acctacatgg cggacgcccg cctgctggga
    32161 ttcgtcgatc tgcgcgccct cgacgaaacc cgcctgaact gggcactgaa cctgatccgc
    32221 gcctacgtcg aaggcgactt cacggcgccg tggccgcgcg cggtcgcgct gtgcgcgctg
    32281 tacgacctga ttccggccga agcggcctga agcaccgtcc gcctttgcct tgcctgcagc
    32341 acgcctgtcg ccgcctcagc gcggcggcag gaagtcgcgc agcgcctcct gcacgtcacg
    32401 cagcgcatag cgcgactgca ccggcacgtg cagcaccgga atgccggccc cggtcagcgc
    32461 ct
//
LOCUS       DAOQIE010000005        32130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_587_length_32130_cov_7.941980, whole genome shotgun sequence.
ACCESSION   DAOQIE010000005 DAOQIE010000000
VERSION     DAOQIE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 32130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32130
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_587_length_32130_cov_7.941980"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PK144_00605"
     CDS             <1..326
                     /locus_tag="PK144_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF64252.1"
                     /translation="LASGDTIEVEDLGLMPVSPGVALSPVTGLAAQPVPMDAAAAARP
                     LPMAPAAEEGANLEAVERRHIEQVLAGCNGVIEGPKGAAAALGLHPSTLRSRMQKLGM
                     RGRRE"
     gene            509..967
                     /locus_tag="PK144_00610"
     CDS             509..967
                     /locus_tag="PK144_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64253.1"
                     /translation="MKIYQQLMASLFAMIFQGLAFALILVAGWQFAGAWREGSEEVLS
                     IVVSGINTLVISLAMYELGVGVGKEYDPDNSPDNAYQTVRRTISRFVGTVCSALVLEG
                     LIMAIKYSQLDLAGNLVYPVALLISAAVLLIALGVFLRLTAPPAVQSEPR"
     gene            967..1464
                     /locus_tag="PK144_00615"
     CDS             967..1464
                     /locus_tag="PK144_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNF64254.1"
                     /translation="MPASAHDRGLVRIGVISDTHGLLRPAALHALRDSRLILHAGDIG
                     DPEILERLAQIAPVLAVRGNNDSGDWAARLPETLITDCVPGLRALIVHDLKTLACDAR
                     AAGCAVVVSGHSHRPLIERRDGVLYLNPGSAGPRRFRLPLSCGWLGLDADGRLHAGIE
                     SLEPD"
     gene            complement(1483..2574)
                     /gene="mnmA"
                     /locus_tag="PK144_00620"
     CDS             complement(1483..2574)
                     /gene="mnmA"
                     /locus_tag="PK144_00620"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNF64255.1"
                     /translation="MKPQRIVVGLSGGVDSAVSAWLLREQGHEVHAVFMKNWDDALDT
                     GWCSAAEDLRDCEDICATLDIPLHRVNFAREYRERVFAAFLSELAAGRTPNPDVLCNR
                     EIKFRAFLDHALALGADALATGHYARTARTADGRVQLLKGLDPGKDQSYFLHALEQRQ
                     LAPACFPVGALPKREVRAIAARLGLVVHDKKDSTGICFIGERDFTQFLAQYLPAEPGP
                     MRTPDGECVGTHRGLAFYTLGQRQGLEIGGRRGGSGAPWFVAGKDRETNTLIVVQGSD
                     HPALLAAGLRASKLSWIGGEAPALPYRCSAKIRYRQADQPCTLLAIDGDTARVRFDVP
                     QRAVTPGQSVVFYAGEVCLGGGIIDAAGG"
     gene            2904..5825
                     /locus_tag="PK144_00625"
     CDS             2904..5825
                     /locus_tag="PK144_00625"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64256.1"
                     /translation="MALQNMEPASPVPGQGHFISLKWRALLLTSIVLVVMIFGGAWLN
                     YDNLSRQLRTQLAQDNAARLRELHALMGQSVERLRQLAGMIPTFPGMQTALRGNDGLA
                     VASVLDAQWPALQFDLGLDRLVVYGSDDASALAQWSVSSGTEVEARLVGLVAMARAQE
                     TPQSLLECSPECMHYVAAPMLAEGRNAGVVLLGVGLADTLRSFAAVSGAEVGLLRIGA
                     RGSRDPALGRLDAWNADLSALTRPEQSLPVLRLAAGRQALEDAAGGLLVEAGERSYDL
                     RVSTLPGLARDHPIRLLVIADITAPLSDIRAAAQTAFGIGAIGWLLAECALLAILWRP
                     MSRLRKTAQTLPLLAQSEFAAVRTTLAGVITPDGRGLRFGHDEIDWLDAITIALADRL
                     EILEAVVRERTEGLARQRDELAAERDFVNSLLDTAQVAILTLDAQLRIVRANSFASAI
                     SGAAREELGGQPFLQLLAPESAGELTMALRGLLAGNEHLRHESLVVHRNGTLRTLTWH
                     HSRLGRSVDGGGPLIVSVGLDNTERRGAEMRLAWLADHDQLTGLPNRPRLQEELELMI
                     AAAARQNRSGALLMVDLDQFRYINEADTHQSGDRLLKAVAVALQRGLPEADFIARLGG
                     DEFAVLLRETDAERACRIGADINQQIANIRCEVAGRPFRLSASIGIVLFPGQGGAEEV
                     LAHGDFAMYQAKEAGRGHWHLFSEADRARERLQDRMLWKDRVASALDEDRIELYFQPI
                     MHIGEGTISHHEVLVRLRDPDGRIHAAGQFIEAAERTGMINALDRRVLTKAIRHLGVI
                     TRSGHDASFALNLSGHTLGDPELLALLRRALDEHDVDPARLIFEITETAAVADFEQAL
                     AVIVAIKSLGCRFALDDFGIGFSSFTYLRHFPVDYLKLDGSFIRQLPDRPEDQAIVRA
                     LHQIAIGFGKRTIAEYVETEATLKLLGEIGIDYAQGYYISEPRPLEVVFGISGPQPES
                     A"
     gene            complement(5756..7987)
                     /locus_tag="PK144_00630"
     CDS             complement(5756..7987)
                     /locus_tag="PK144_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64257.1"
                     /translation="MNDPAAAGTLRLLYIDDDPEQRLLVRLALERLGGWQITLAGSAD
                     AQLPALAQRTGAGCVLLATAPDPAATEAVLALLRADERSAALPVVILAVLAPPAAAAR
                     WRSLGALEAITGPLVPSALPARLRALRTVRRTEPGAAAPAAPEADADPIADLRRSFLA
                     QLPERRQALRSHWQSAAGGDPAAADEVRLIAHRLAGTGATFDQPAVGTQARRVETALL
                     AWRARPGETDLRAAVDTALADFEAAVSAALGPSAAADAAPASTDAPPPTPTSAPTAPP
                     ASPRPARSEASTDPQPGTAEAGTGAGPEVKAEPSATATLWAVGIAPMLLTTLSPLELQ
                     LRTFADLDAALSALEAGVTAPAATLIGTAMLGRPEAIARLRARAPLALYSGRGSLADR
                     IAGVHAGAETCFAATVDQALLGEWLEMRCVPRVGDALRVLLIDDDPIFIALCSNLLSG
                     NGFVVRAMEHPYALCEELAEFEPDVLLLDLYLRDCTGLEVATALRQFPGWSNLPIVFL
                     SADHELAHQAAALGAGLDDWLSKPFEAVQLRQVVQTRALRGRRLRARVDTDPLTGVLS
                     ASSFENRLGIEVGRAQRYGRALSLVLIDPDELGALADAQGRGAADRLLCLLAQQLGQR
                     VRRSDLLGRGHGAAFALALTETGGDVAGCVIETLRSTWQRSAADAAQPLALAGFSAGL
                     AALRPGENAPALHERALRALRAARRAGGNRLLRMTELGDQADSGCGPEMPKTTSSGRG
                     SLM"
     gene            complement(7980..10991)
                     /locus_tag="PK144_00635"
     CDS             complement(7980..10991)
                     /locus_tag="PK144_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64258.1"
                     /translation="MLLTGLGCFAAAVICILLSRGSSPLATIWLPNAILLAVALRAPY
                     RQIWPVVITGAAAGLCANLATDVSPGLAAGYAFANLVSVLVPLLALRPADSQPFLLTE
                     RPTSAVRLILVVATGTMLSALVGGALLATVHARPLPASIGNWLTSDLTGMVAVLPLGL
                     RASLRESHYWFAAGRRTEILLLYGLTIAGTFASLQLSPFPFVTIELPLLLASFRLGLG
                     GTALLCACESLLFAALLSVLPPVPMQMALQTYGPGINALAQFAPLAVAWLLECRSADM
                     GRLRASEERLRSAMRDSAIGMALIDLEGRFVAVNPALCALGGHEAQALLGHHFADFIH
                     GPDRYVAQELARQLAEGTIGSTRYELRGLRADGSLHWLRVVASCMRDGQQRPTGFIAQ
                     IEDIDAQRQAERARTEQAALIERAHDAIIGRDAEGRVTHWNRGAERLYGWSAAEMTGQ
                     PLHERLDTHNAVSGAADEPERTALDYWEGTLLQRHRDGHALTIESRRVLLRGPDGRLS
                     ATLEINRDVSTLREAVHLHAQFVALVARELRAPLTTIGGTLDLIASGALGTVSGEAQD
                     LIDIARRHDARLMRIVADIQDAERIAAGDVPLAPQTIALAALLGQLRDEYAAAAAAAG
                     IRIDIDAAGELSVNADPERLTQVLGHLLDNALRHAPAGSAVTLRALKLPGRRAGIEVC
                     DRGAGVPAELRPRLFHRFLQGPVETGRPAGGAGLGLYIARELTERQGGTINYETGPEG
                     TCLHLELPLAVVTSTHSERPHVLVVEDDALAASAFVAELSRDGYIAEVAGSVRAAREK
                     LAVGRYALVTVDMLLPDGMGEEVIRAMREDPRHVAVPVMVVSALPQGSLEAVALPVAD
                     WLPKPVRPERLQAAIAALGVHAERAQVLHVENDPGMREYVRRLLGPRVKLFDAETLSE
                     AEHLAATLPFDLAILDLLLPDGYGLAVAPKLHTAGGEPTPVLVFSAGEPDPAQWQAIA
                     GTLSKASATGKDLLRTVGSLLGRDREGPPHE"
     gene            complement(11244..11663)
                     /locus_tag="PK144_00640"
     CDS             complement(11244..11663)
                     /locus_tag="PK144_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF64259.1"
                     /translation="MTETASTSAGDRAAVVTVRPEAEVLTRQRLPYFVGICAATAGAR
                     GLAMHLVVIPPGAASEPHLHRGYETAIYILEGRVETRYGRRLEHRVVNVAGDFVYIPA
                     DLPHQAVNLSATEPARALVARNDAADREHVDPWQAPV"
     gene            complement(11727..12632)
                     /locus_tag="PK144_00645"
     CDS             complement(11727..12632)
                     /locus_tag="PK144_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF64260.1"
                     /translation="MELAWLEDFLAVIDCGSFSRAADSRHVTQPAFSRRIRSLEDWIG
                     TPLFERSSHGAALTAAGERFRPSAEAALRKLLHGREDAIAAADSGLPLLRFASTHALA
                     LDFFPQWLRKVEARSPVDASIRLIADTMHACEQLLLRGEAQFLLCHGHAGVSGRLDNG
                     DYRSITVGNDVLLPVSAPRAANDPRPRFELPGSPDEPLPLLAPTEASGLSRALAAAQA
                     QLAPAQVRPAFTAHLATVLAAMARDGRGLTWVPQRLVADDLAAGRLVHAGDASWTIPM
                     SIRLYRPRNLTGAAATRLWAAAGGD"
     gene            12866..13636
                     /locus_tag="PK144_00650"
     CDS             12866..13636
                     /locus_tag="PK144_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF64261.1"
                     /translation="MPILHTDFLLPALALGVALGWLGGLFGIGGGLIAIPVLGLACGL
                     DQLHAQGTALVMVLPNVVIGFWRYRARHGIAWRPALLLGLTAIAGSEFAAHLATGLPA
                     VTLRLMFAGFLIVLAGWLLRGLRRAPPATVAPAPAVLSARWLPAVGLAGGLMSGLFSV
                     GGGIAAVPLLTGLFGTRQAVAQGLALALVTPGSAAALFAYARAGAVDWPLGLALAFGG
                     VLSVSAGVAFAHRLPERRLRALFALGIVATAGAMLASA"
     gene            complement(13646..14617)
                     /locus_tag="PK144_00655"
     CDS             complement(13646..14617)
                     /locus_tag="PK144_00655"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNF64262.1"
                     /translation="MSSSASDRTPDLIADIGGTNARFALSDGPGSTPRAVQVLATSAY
                     ADLAGAADAYLRHAGGPRPQRAAVAIANPVTGDRVEMTNNAWRFSREATRTALGLERL
                     ELLNDFKALALSLPLLAGEALQQVGGTVPVAGTAIGLIGPGTGLGVSGLLPDGHGGWR
                     AIEGEGGHVSFSPADALELEILRWALARHPHVSVERLVSGALGLPNLHQALAEIDGLP
                     AEAMAAPEIVQRGTSGDCALCRRVLTVFCAMLGTAAGNLALTLGARGGVYIGGGIVPR
                     FPEFFAASPFRARFEHRGRFTAYLQPIPCFVIHAPDAALRGAAAALD"
     gene            complement(14720..15346)
                     /locus_tag="PK144_00660"
     CDS             complement(14720..15346)
                     /locus_tag="PK144_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HNF64263.1"
                     /translation="MFTGIVQAAVPVVALVERPQLVTLTLELPERLCTGLAIGSSVAV
                     NGTCLTITGFEGRLVHFDAMLETLRVTNLGRLAVGDRVNVERAARHGDEIGGHVLSGH
                     VHALAEIIRIERPENNHEVVFRAPAALMKYLLPKGFVALNGCSLTIGRVEGDTFDVWL
                     IPETLRATTYGELQVGALVNLEIDAQTQAIVDTVERVLAERGLLAAAG"
     gene            complement(15499..17367)
                     /locus_tag="PK144_00665"
     CDS             complement(15499..17367)
                     /locus_tag="PK144_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF64264.1"
                     /translation="MGDNNNEPVFDIALTMAGAVSAGAYTAGVLDFLVEALTAFEAEK
                     ARQPGRYPWRVRIRVISGASAGSIVGAIFASTLRPGVTPVRTQHPAAGASNPLFDAWV
                     ERVDISGLLGTADLRQGPLSSLLDSTALDRIAAQALAVDWAAGAASLPAFVSQPLDLY
                     FTLNNQRGVPYGIDFRGDAAQAVYGMTLHADSIRFRLDPGSTASEDGETIVLGRQNGT
                     GGGWGQLGQAALASGAFPVGLAPRALKRTQAEIYNQRLWTLPEFHAARPDVIARCTVQ
                     ARIPTALPATVCTRAYDYVNVDGGTIDNEPFEIGRRTLAEDHGREHNPRSPHEADCAV
                     LMIDPFPCPPVFTEDYQVPEGLKLIPAIKHLLSAAIQQMRFKLPELMLADHPDVYSRF
                     LITPVRYAADGRRVQLPGTPIACGTLGGFGGFLNRAFRAHDYLLGRLNCQQFLRRHFV
                     LAGDNPLFGGTAGPDSDYDKRFGRKPADPDLYDGAPVQLVPILPLFGSAAGAAIDGFQ
                     PQAPFGLPWPQIRESELQPVQGGLKTRADAVVGALLKENVRSVLGRLALNSAWSVFGR
                     RRVLDKAMDMIRDALAVAGLLAGVAPPPVPTGGPDRTLPPVRSLDAGAPGGKVSNG"
     gene            complement(17555..17797)
                     /locus_tag="PK144_00670"
     CDS             complement(17555..17797)
                     /locus_tag="PK144_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HNF64265.1"
                     /translation="MSSYDEQEDTTLYAVVVNHEEQYSIWPADRELPLGWTHEGTQGN
                     KQTCLAHIEQVWTDMRPLSLRRKMEEDARRAAESGD"
     gene            complement(17907..18209)
                     /locus_tag="PK144_00675"
     CDS             complement(17907..18209)
                     /locus_tag="PK144_00675"
                     /inference="COORDINATES: protein motif:HMM:NF015578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HNF64266.1"
                     /translation="MGVVVDTAIRYAVMIGSNGEYSLCRAERCPPPGWRLEGTEGTRS
                     ECMACVQAHAAPAVFAAAPDAGGDDVPAADDVQPPALPAGRRRSRPAHTGRDSLTN"
     gene            complement(18367..19257)
                     /gene="draG"
                     /locus_tag="PK144_00680"
     CDS             complement(18367..19257)
                     /gene="draG"
                     /locus_tag="PK144_00680"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HNF64267.1"
                     /translation="MYDSDVRDRALGAYLGLALGDALGATVEFLTAREIAAQHGRHDE
                     IKGGGWLKLRAGAVTDDTQMSMALGLALIESGGWQLTAIAEAWAEWLRSKPADVGNTC
                     RRGIRRYLKDGSLLTPESADAGNGAAMRNVPVTIVTLGHPRALVKRSLEQARFTHHNP
                     LSDAATVTLGQMTQTLMLGGTTADCRLQADTLIASHRQFRFEPYPGQAGGYIVDTVQT
                     VFDAFFAEEDFEACLIRTVNRGGDADTTGAIVGQLAGARCGASRLPRRWLKKLDPSVH
                     AAIVAQTDGLLALAAAAAAC"
     gene            complement(19281..20141)
                     /locus_tag="PK144_00685"
     CDS             complement(19281..20141)
                     /locus_tag="PK144_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HNF64268.1"
                     /translation="MSLTRTGTENTPHTPEPTPLSRIGHSTPLVGVPAAVIASLAYND
                     APVPLVIPAVRETNRALYSKLAECPDARSAGQIFQDYMDVVFGLARPDEPACGACRRF
                     RASYLRLLKGWAFDASNPEGAVLKGWVESRFGLFPTWHKAALTRFSSPQWIAYVEEKM
                     APRFHNNAVHAQLDLLYSFCQWRLEHFPLLPPPARHLRLYRGVNDLTEHPLVQRIDKR
                     TAQLRLNNLSSFSADRDRAGEFGDCILEAEVPLTKILFFNDLLPKHMLKGEGEYLVIG
                     GDYRVRTSWL"
     gene            20743..21618
                     /gene="nifH"
                     /locus_tag="PK144_00690"
     CDS             20743..21618
                     /gene="nifH"
                     /locus_tag="PK144_00690"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HNF64269.1"
                     /translation="MSTLRQIAFYGKGGIGKSTTSQNTLAALAEMGQKILIVGCDPKA
                     DSTRLILHAKAQDTVLSLAAEAGSVEDLEIEDVMKIGFRDIRCVESGGPEPGVGCAGR
                     GVITSINFLEENGAYDGVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
                     AANNISKGILKYASSGGVRLGGLICNERKTDKELELAEALAGKLGTKLIHFVPRDNVV
                     QHAELRRMTVIEYDPNCKQAGEYRQLAEKVHNNAGKGVIPTPITMDELEDMLMEFGIM
                     TKEDESIVGKAASAA"
     gene            21763..23217
                     /gene="nifD"
                     /locus_tag="PK144_00695"
     CDS             21763..23217
                     /gene="nifD"
                     /locus_tag="PK144_00695"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HNF64270.1"
                     /translation="MSSTAEDIKARNKALIAEVLEVYPEKAKKKRQKHLNAYEGGKPD
                     CGVKSNIKSLPGVMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWASRRNYFI
                     GTTGIDSFGTMQFTSDFQEKDIVFGGDKKLEKIIDEIQVLFPLNKGISIQSECPIGLI
                     GDDIEAVAKKKSKEYEGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKSTGEIEG
                     FVPTPYDVTIIGDYNIGGDAWSSRILLEEMGLRVIAQWSGDGTLAELEKTPKAKLNLI
                     HCYRSMNYISRHMEEKYNIPWLEYNFFGPTKIEESLRAIAAHFDDTIKANAERVIAKY
                     KALTDAVIAKYRPRLEGKKVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQR
                     TTHYVKDGTLIYDDVTGFEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYS
                     GPYHGYDGFAIFARDMDMAINSPVWGLTKAPWKK"
     gene            23289..24827
                     /gene="nifK"
                     /locus_tag="PK144_00700"
     CDS             23289..24827
                     /gene="nifK"
                     /locus_tag="PK144_00700"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HNF64271.1"
                     /translation="MSQNAEKVLDHFKLFREPEYVEMFESKKKNFEDAVPAEELERVR
                     EWAKSWEYREKNFAREALTVNPAKACQPLGAVFAAVGFESTLPFVHGSQGCVAYYRSH
                     FSRHFKEPTSCVSSSMTEDAAVFGGLQNMIDGLANSNAMYKPKMIAVSTTCMAEVIGD
                     DLNAFIKTAKEKGSVPMDMDVPYAHTPAFVGSHVTGYDNSMKGVLTHFWDNAERTPNE
                     SINLINGFDGYVVGNIRELKRVLGLMGVDYTLIGDQSDVWDTPTDGEFRMYDGGTKIE
                     DVKAAVNAKATLSMQEYCTEKTLAYCADKGQQTVALNHPVGIAGTDRLLMALSELTGK
                     EIPAELLKERGRLVDAIADSQAHIHGKSFAIYGDPDLCYGLTEFLLELGAEPKHVLST
                     NGNKAWAEKMQALFNSSPFGKGCVAYAGKDLWHMRSLLATEPVDFLIGNTYGKYLERD
                     IGTPLIRIGFPVFDRHHHHRSPIWGYQGGMNLLVKLLDKIFDEMDKDTNIPSKTDYSF
                     DIIR"
     gene            25041..25388
                     /locus_tag="PK144_00705"
     CDS             25041..25388
                     /locus_tag="PK144_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF64272.1"
                     /translation="MANVTFSSPSMKKDVTVYATAGDTQTLLAVAKQHKIPVHFECEN
                     GECGSCVVQVTVLTANAPYAIALTEKEKAVLKLAGKITPEQIDNAEVNDVPPPYRLAC
                     QFIVRNEDILVKF"
     gene            25599..27446
                     /locus_tag="PK144_00710"
     CDS             25599..27446
                     /locus_tag="PK144_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF64273.1"
                     /translation="MSAADTATELSLALWALKRALARQDAHAQPDIDRLRVDAAYRDT
                     IVAEAGQSPHEAVREAAARVRALISAAVQEARQDKAARRGARTALVAGMVLVLAAAGV
                     LGWQWQTLQPRQGRTPVVASVAPQPAAVVTPAAIPAAVTVPKPVTQAAAPLAAEAANT
                     PVQLLSRPEDIAPAAAGSVPAASAALPVVPVAALPAAAPVKLRIHGSNTIGAELAPAL
                     VERFLARRGASDVRRVPGARAEEMRIEATLPGSEGRIAIELAAHGSGTAFTDLADGRA
                     DVGAASRPVRDEEIRKLAALGDFSSPAAEHVIGLDGVAIIVHPANPLRSLSREQVAKL
                     FAGEIRDWAELGGRPGPVHIYARDERSGTWDTFQSLVLGKAYPLSPEAQRFESSPELS
                     DRVATDAGGIGFIGLPYVRMARALAIGDGNAVPSAPSPFTVATEDYPLSRRLFFYLAP
                     TGDNRMARELVEFALENDGQQVVEAHGFISQRVAPQRVALSPNAPKEYESLTRDALRL
                     SVNFRFRPGSGLLDGKAQRDLRRVTDYLAQAENRSRQVLLFGFTDARGSPEQNLRLSQ
                     QRAQEIARELQARGIQTRVVRGFGPANPVAADDSPSGQERNRRVEIWVM"
     gene            complement(27450..28238)
                     /locus_tag="PK144_00715"
     CDS             complement(27450..28238)
                     /locus_tag="PK144_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64274.1"
                     /translation="MSYRSRHCLALLVLVSGTALPAFAGPWLALCAKCLNPTVFRTQG
                     LDTAHAVAEARITREAAAGWCENWEPGRPVAACVAEQMAGDDAKRIWRAEADCTAGRI
                     TPVDGETYTFAGVWRSDVGRGRTRWRDAQGRIVGQDNASGGLGIAQQWEVLCPRTGTR
                     PGSAGGRAPVPAPQPGAGTGEPPPANGGGHGSGGFDAFFPAPAPAPEYRVGQAVEAKY
                     LSDWLPARIEAVRPARRGGFEYDVRLANGKRGAGVPPRLLRPAP"
     gene            complement(28291..29028)
                     /locus_tag="PK144_00720"
     CDS             complement(28291..29028)
                     /locus_tag="PK144_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF64275.1"
                     /translation="MPALHHIEAGSGEALVLLHSGGMCHEEWKPQLPAFAKHHRTIAP
                     DLPGHGASPMRAAHLSIGDCGRGVLDTLDALGIERAHLVGSSMGGATALWLAVHHPAR
                     VAKLVLYRVSYHKNEHTHGGTREMADAAYWRGVGLAGWLSRIHQPQGGPDAWEQVITR
                     VSEALDPATSDHAHTIETLRALRCPTLVVCGDRDPLVPMDDVLAMYDHIPDCGLWLLP
                     HATHVTATNTWRAEAFATEILRFLQRR"
     gene            29111..29965
                     /locus_tag="PK144_00725"
     CDS             29111..29965
                     /locus_tag="PK144_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF64276.1"
                     /translation="MRTGSIRALSPRGFHTVRYVEWGEADNPEVLFCVHGLTRNGRDF
                     DRLARALAADYRVICPDVVGRGASDWLANKLDYGYPQYLTDLVALIARSGAEQVDWLG
                     TSMGGLIGLMLAAQPNHPIRRLVLNDVGPLIPKESLERIAAYVGAPQVFDTREDFELY
                     ARAIWAPFGLTSDGEWQDLLAHSGRRTGDGRWAFNYDPGIAEPFRQMAMADIDLWPLW
                     DRIRCPTLLLRGETSDVLPRSVAREMTTRGPKARLVEFPNCGHAPALMNAEQIATVRD
                     WLTTAETA"
     gene            30006..30893
                     /locus_tag="PK144_00730"
     CDS             30006..30893
                     /locus_tag="PK144_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNF64277.1"
                     /translation="MIELLLRIGGIVFPVFAIAAVGALWGRRHRPDMSVVNRLNVEVF
                     APALVFWALADKPLDFDLYRDLAIGGIAVVLGSGLLLWPFVRLAGIDARTFIPPMMFN
                     NSGNMGIPLALFAFGETALQAAVVLFIIEMVLHFTVGLYILDHRTRPWRLLKMPMIQA
                     TIAGLACSAFGLTLPAPLANTIKLMGQVSIPLLLFALGVRLLDVNLRDWKLGTAGAFA
                     GPLAGVASLWLVLPWLALDEAQFPQLLIFAALPPAVLNVLVAEQYRQEPERVASIVLI
                     GNLGSLVVLPAALWFALPH"
     gene            30954..31328
                     /locus_tag="PK144_00735"
     CDS             30954..31328
                     /locus_tag="PK144_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNF64278.1"
                     /translation="MTQRQHNVLGEPLAVCSVLPMTGFTRNGCCDAHSGDTGSHTVCV
                     RVTAEFLRFSRACGNDLSTPLPAWGFPGLRPGDRWCLCAARWQEALEAGVAPPVLLAA
                     THARALEHVRLDDLRRHALDPA"
     gene            31485..32012
                     /locus_tag="PK144_00740"
     CDS             31485..32012
                     /locus_tag="PK144_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNF64279.1"
                     /translation="MHTSLVLTVIGPDRPGLVEAIAAVVARHGANWEESRMAQLGGQF
                     AGMLRVTAPEETQDALEDALAGLGSVGLKVTVARAAGAQAQVPKLLHLNLVGQDRPGI
                     VREIAEALARRGVSIESLETRCVSASMSGEMLFEAEAVLAVPPALAADELRTALEALA
                     NELMVDLDLAEPAGA"
BASE COUNT         4780 a        11004 c        11577 g         4769 t
ORIGIN      
        1 tcctcgccag cggcgacacc atcgaggtcg aggatctcgg gctgatgccg gtatcacccg
       61 gcgtcgcgct cagcccggtg acggggctgg cggcgcagcc ggtgccgatg gatgccgccg
      121 ccgcggcgcg cccgctgccg atggcgccgg cggcggagga aggcgccaac ctcgaagccg
      181 tcgagcgccg ccacatcgag caggtgctcg ccggctgcaa cggcgtcatc gaaggcccga
      241 agggcgcggc ggcggcactg gggctgcacc cgagcaccct gcggtcgcgg atgcagaagc
      301 tcggcatgcg cggcaggcgc gagtgatgcc cgctgccgcg ctgtcctgcc catcggcccg
      361 gccggcgcgc cggtggcatc cgcctgcggg cggggagcat gcggatgttc cgccggccat
      421 cctgccggct cgcgggcggt ggaatctttc gtgtccgttg cggccccagt tcaccgaccc
      481 gcctgtgcag cctgaggatt ccgcaaccgt gaagatttac cagcaactga tggccagcct
      541 gttcgcgatg atcttccagg ggctggcgtt cgcgctgatc ctggtcgccg gctggcagtt
      601 cgccggcgcc tggcgcgagg gcagcgagga ggtgctgagc atcgtcgtca gcggcatcaa
      661 cacgctggtg atctcgctgg cgatgtacga actcggcgtc ggcgtcggca aggagtacga
      721 ccccgacaac tcgccggaca acgcctacca gaccgtgcgc cgcacgatca gccgcttcgt
      781 cggcacggtc tgcagcgcgc tggtgctcga aggcctgatc atggcgatca agtacagcca
      841 gctcgatctg gccggcaacc tcgtctatcc ggtcgcgctg ctgatctcgg ccgcggtgct
      901 gctgatcgcg ctcggcgtgt tcctgcgcct gaccgcgccg ccggcggtgc agtcggagcc
      961 gcgctgatgc cggcgtccgc gcacgaccgg gggcttgtgc gcatcggcgt catttccgac
     1021 acgcacgggc tgctcaggcc ggcggcgctg catgcgctgc gcgacagccg gctgatcctg
     1081 cacgccggcg acatcggcga tccggaaatc ctcgaacggc tcgcgcagat cgccccggtg
     1141 ctcgccgtgc gcggcaacaa cgactccggc gactgggccg cacgcctgcc cgaaaccctg
     1201 atcaccgact gcgtgcccgg cctgcgtgcg ctgatcgtgc acgacctgaa aacgctcgcg
     1261 tgcgatgcgc gggccgccgg ctgcgcggtc gtggtcagcg gtcacagcca ccggccgctg
     1321 atcgagcggc gcgacggcgt gctgtacctg aaccccggtt cggccggtcc gcgccgcttc
     1381 cggctgccgc tcagctgcgg ctggctcggg ctcgatgctg acggccggct gcacgccggc
     1441 atcgagtcgc tggagccgga ctgacgcgcg cgccgcgcag gctcagccgc cggccgcgtc
     1501 gatgatgccg ccgccgaggc agacctcgcc ggcgtagaac accaccgact ggcccggcgt
     1561 cacggcgcgc tgcggcacat cgaagcgcac ccgggccgtg tcgccgtcga tcgcgagcag
     1621 cgtgcagggc tggtcggcct gccggtagcg gatcttcgcg ctgcagcgat acggcagcgc
     1681 cggcgcctcg ccgccgatcc acgagagctt cgaggcgcgc aggcccgcgg cgagcagcgc
     1741 cggatgatcg ctcccctgca cgacgatcag cgtgttggtc tcccggtcct tgccggcgac
     1801 gaaccacggt gcgccgctgc cgccgcgccg gccgccgatc tccaggccct gacgctggcc
     1861 gagcgtgtag aaggccaggc cgcggtgcgt gccgacgcac tcgccgtcgg gcgtgcgcat
     1921 cgggccgggc tcggccggca ggtactgcgc gaggaactgc gtgaagtcgc gctcgccgat
     1981 gaagcagatg ccggtcgaat ccttcttgtc gtgcacgacc aggccgaggc gcgcggcgat
     2041 ggcgcgcacc tcgcgcttgg gcagcgcgcc gaccggaaag caggccgggg cgagctggcg
     2101 ctgttcgagc gcgtgcagga agtagctctg gtccttgccc gggtcgaggc ccttcagcag
     2161 ctgcacgcga ccatcggcgg tgcgggccgt gcgcgcgtag tggccggtgg cgagcgcatc
     2221 ggcaccgagg gccagcgcat ggtcgaggaa ggcgcggaac ttgatctcgc ggttgcagag
     2281 cacgtcgggg ttcggcgtgc gtccggcggc gagttcggac aggaaggccg cgaacacgcg
     2341 ctcgcggtat tcgcgcgcga agttcacgcg gtgcagcggg atgtcgagcg tcgcgcagat
     2401 gtcctcgcag tcgcgcagat cctcggcggc gctgcaccag ccggtgtcga gcgcatcgtc
     2461 ccagttcttc atgaacacgg cgtggacctc gtggccctgc tcgcgcagca gccacgcgct
     2521 gacggcggaa tcgacgccgc cggacaggcc gacgacgatg cgctggggtt tcatgcgaag
     2581 gaaaatccgg ttcgggcgga aaggcggccg gcgggccggg gagggatgca gcggccatga
     2641 taccggcacg ccgtgcggat gccgtactga agcccggcgc cgcgcagccg atgcccgggc
     2701 gatgggctgg gcgctgccgt ttccgccggc cggcggcttg ccgggtcacc ggcgtccggg
     2761 gcagcctgtg cagtctgcaa ggcatgctgc actgcgacat gcgatttgac ggttgcaagg
     2821 ccaccggatg gcataccctg tccacaccgg tccgaccaca cggacatcgc cttttcagca
     2881 gtcgggcatc ggcccaccgg cgcatggcat tgcagaatat ggaacccgca tcgcccgtcc
     2941 ccggccaggg tcacttcatc agcctcaaat ggcgcgcgct gctgctgacc agcatcgtgc
     3001 tggtcgtgat gatcttcggc ggggcctggc tgaactacga caacctgagc aggcagctgc
     3061 gcacgcagct cgcgcaggac aacgccgcac gcctgcgcga actgcatgcg ctgatgggcc
     3121 agtcggtcga gcgcctgcgt cagctcgcgg gcatgatccc gacctttccg ggcatgcaga
     3181 cggcgctgcg cggcaacgac gggctggcgg tcgccagcgt gctcgatgcg cagtggccgg
     3241 ccctgcagtt cgatctcggc ctcgaccggc tcgtggtcta cggcagcgac gacgccagcg
     3301 cgctcgcgca gtggtcggtg agttcgggca ccgaggtcga ggcgcggctg gtcgggctgg
     3361 tggcgatggc gcgcgcgcag gagacgccgc agagcctgct cgaatgcagc cccgagtgca
     3421 tgcattacgt cgccgcaccg atgctcgccg agggccgcaa cgccggcgtc gtgctgctcg
     3481 gcgtcggact ggccgacacg ctgcgcagct tcgccgccgt ctccggcgcc gaggtcgggc
     3541 tgctgcgcat cggcgcccgc ggcagccgcg atccggcgct cggccggctc gatgcctgga
     3601 acgccgacct ctcggcgctg acccgtcccg agcagtcgct gccggtgctg aggctcgcgg
     3661 ccggccggca ggcgctcgag gatgccgccg gcggcctgct ggtcgaggcc ggcgagcgca
     3721 gctacgacct gcgggtgtcg acgctgccgg gcctggcccg cgaccatccg atccggctgc
     3781 tggtgatcgc cgacatcacg gcgccgctct ccgacatccg cgccgccgca cagacggcct
     3841 tcggcatcgg cgcgatcggc tggctgctcg ccgaatgcgc gctgctcgcg atcctgtggc
     3901 ggccgatgtc gcggctgcgc aagacggcgc agacgctgcc gctgctcgcg cagagcgagt
     3961 tcgcggcggt gcgcacgacg ctggccggcg tcatcacgcc ggacggccgc ggcctgcgct
     4021 tcgggcacga cgagatcgac tggctggatg cgatcacgat cgcgctggcc gaccggctgg
     4081 agatcctcga agccgtggtg cgcgaacgca ccgagggtct ggcccgccag cgcgacgagc
     4141 tcgccgccga gcgcgatttc gtcaacagcc tgctcgatac cgcgcaggtc gcgatcctga
     4201 cgctcgatgc gcagcttcgc atcgtgcgcg ccaacagctt cgcctcggcg atcagcgggg
     4261 ccgcgcgcga ggaactcggc ggccagccct tcctgcagct gctggcgccg gagtcggccg
     4321 gcgagctgac gatggcgctg cgcgggctgc tcgccggcaa cgagcacctg cgccacgaat
     4381 cgctggtggt gcatcgcaat ggcacgctgc gcacgctgac ctggcatcac tcgcggctcg
     4441 ggcgcagcgt cgacggcggc gggccgctga tcgtctcggt cggtctcgac aacaccgagc
     4501 ggcgcggtgc cgagatgcgg ctcgcctggc tggccgatca cgaccagctc accggcctgc
     4561 cgaaccgtcc gcgcctgcag gaggaactgg agctgatgat cgcggccgca gcgcgccaga
     4621 accgcagcgg cgcgctgctg atggtcgatc tcgaccagtt ccgctacatc aacgaagccg
     4681 acacccacca gagcggcgac cggctgctga aggccgtggc ggtggcgctg cagcgcgggc
     4741 tgccggaggc cgacttcatc gcccgcctgg gcggtgacga gttcgcggtg ctgctgcgcg
     4801 agaccgacgc cgagcgcgcc tgccgcatcg gcgccgacat caaccagcag atcgccaaca
     4861 tccgctgcga ggtcgccggc cggccgttcc ggctgtcggc gagcatcggc atcgtgctgt
     4921 tccccgggca gggcggggcc gaggaagtgc tcgcgcacgg ggatttcgcg atgtaccagg
     4981 ccaaggaggc cggtcgcggc cactggcatc tgttctcgga ggccgaccgg gcgcgcgagc
     5041 gcctgcagga ccgcatgctg tggaaggacc gcgtcgcctc ggcgctcgac gaggaccgca
     5101 tcgagctgta cttccagccg atcatgcaca tcggcgaggg cacgatctcg caccacgagg
     5161 tgctggtgcg cctgcgcgac ccggacgggc gcatccacgc cgccgggcag ttcatcgagg
     5221 cggccgagcg caccggcatg atcaacgcgc tggaccggcg cgtgctgacc aaggcgatcc
     5281 gccatctggg tgtgatcacg cgcagcggcc acgacgcgag cttcgcgctg aacctgtccg
     5341 gccacacgct cggcgatccg gagctgctgg cactgctgcg ccgtgcgctc gatgagcacg
     5401 acgtcgatcc ggcgcggctg atcttcgaga tcaccgagac cgcggcggtc gccgacttcg
     5461 agcaggcgct ggcagtcatc gtcgcgatca agagcctcgg ctgccgcttc gcgctcgatg
     5521 acttcggcat cggcttctcg tcgttcacgt acctgcgcca cttcccggtc gattacctga
     5581 agctcgacgg gtcgttcatc cgccagctcc ccgaccgccc cgaggatcag gccatcgtca
     5641 gggcgctgca ccagatcgcg atcggcttcg gcaagcgcac gatcgccgaa tacgtcgaga
     5701 cggaagcgac gctgaagctg ctcggcgaga tcggcatcga ctatgcgcag ggctactaca
     5761 tcagcgagcc gcggccgctg gaggtcgtct tcggcatttc cggcccgcag ccggaatcgg
     5821 cctgatcgcc gagttcggtc atgcgcagca ggcggttgcc gccggcacgg cgtgcagccc
     5881 gcagcgcccg cagcgcccgc tcgtgcaggg caggggcgtt ttcgccggga cgcagtgcgg
     5941 ccagtccggc gctgaaaccc gccagggcga ggggctgggc cgcgtcggcg gcgctgcgct
     6001 gccaggtgct gcgcagcgtc tcgatcacgc agccggccac gtcgccgccg gtctcggtca
     6061 gggccagcgc gaaggcggcc ccgtggccgc gtccgagcag gtcgctgcgg cgcacgcgct
     6121 ggccgagctg ctgcgcgagc aggcacagca ggcggtcggc ggcgccgcgg ccctgggcat
     6181 cggcgagcgc gccgagttcg tccggatcga tcagcaccag cgacagggcc cggccgtagc
     6241 gctgggcacg gccgacctcg atgccgaggc ggttctcgaa gctgcttgcc gacagcacgc
     6301 cggtcagcgg atcggtgtcg acccgggcgc gcaggcggcg gccgcgcagc gcgcgcgtct
     6361 gcacgacctg acgcagctgc acggcctcga acggcttcga cagccagtca tcgagcccgg
     6421 ccccgagggc agcggcctga tgcgccagct cgtgatcggc cgacaggaac acgatcggca
     6481 ggttcgacca gcccgggaac tggcgcaggg cggtggcgac ctccaggccg gtgcagtcgc
     6541 gcaggtacag atcgagcagc agcacatccg gctcgaactc ggccagctcc tcgcagagcg
     6601 cgtacgggtg ttccatcgcc cgcaccacga aaccgttgcc ggacagcagg ttgctgcaca
     6661 gtgcgatgaa tatcgggtcg tcgtcgatga gcagcacgcg cagcgcatcg ccgacgcgcg
     6721 gcacgcagcg catctccagc cattcaccga gcagggcctg atcgacggtc gcggcgaaac
     6781 aggtctcggc cccggcatgc acgccggcga tgcggtcggc gaggctgccg cgaccgctgt
     6841 acagcgcaag cggtgcgcgt gcgcgcagcc gggcgatggc ctcgggccgc ccgagcatcg
     6901 cggtgccgat cagcgtcgcg gccggggccg tgacgccggc ctccagcgcc gacagcgcgg
     6961 catcgaggtc ggcgaaggtc cgcaactgca gttcgagcgg cgacagggtc gtgagcagca
     7021 tcggtgcgat gccgacagcc cacagcgtgg ccgtggcact gggctcggct ttgacttcgg
     7081 gcccggcccc ggtcccggcc tcagctgtgc cgggctgcgg gtcggtggag gcttcactgc
     7141 gcgccggtcg gggagatgcc ggaggcgcgg tcggcgcaga ggtcggcgtt ggcggcggcg
     7201 cgtcggtgct cgccggcgcg gcatccgccg ctgcagacgg cccgagggct gccgacaccg
     7261 cggcctcgaa gtccgccagc gccgtgtcga ccgcggcgcg cagatcggtc tcgccgggcc
     7321 gggcacgcca ggcgagcagc gcggtttcga cgcggcgggc ctgggtgccg accgccggtt
     7381 ggtcgaaggt tgcgccggtg ccggcaaggc ggtgggcgat caggcgcacc tcgtcggccg
     7441 ccgccggatc gccgccggcc gccgactgcc agtggctgcg cagggcctgg cgccgttcgg
     7501 gcagctgcgc aagaaagctc cggcgcaggt cggcgatcgg gtcggcatcg gcttcgggcg
     7561 cagccggcgc cgcggcgccg ggctcggtgc gccggaccgt gcgcagcgcc cgcagcctag
     7621 ccggcagcgc gctcggcacc agcggtccgg tgatcgcctc cagcgcaccg agcgagcgcc
     7681 agcgcgcggc ggccgccggc ggtgccagca cggcgaggat caccaccggc agcgccgcgc
     7741 tgcgctcatc ggcccgcagc agggccagca cggcttcggt cgctgccggg tcgggtgccg
     7801 tcgcgagcag cacgcagccg gcgccggtgc gctgcgcgag cgccggcagt tgcgcatcgg
     7861 cgctgccggc cagcgtgatc tgccagccgc cgagccgttc gagcgcgagg cgcacgagca
     7921 ggcgctgttc ggggtcgtcg tcgatgtaca gcaggcgcag cgtgccggcg gcggcaggat
     7981 cattcatggg gcggtccctc gcggtcgcgc ccgagcagcg agccgaccgt gcgcagcagg
     8041 tccttgccgg tggcgctcgc cttgctcagg gtgccggcga tcgcctgcca ctgggcgggg
     8101 tcgggttcgc cggccgagaa caccagcacc ggcgtcggct cgccgccggc ggtgtgcagc
     8161 ttcggcgcca cggcgaggcc gtagccatcg ggcagcagca ggtcgaggat cgccagatcg
     8221 aacggcagcg tcgcggccag gtgctcggct tcggataggg tttcggcatc gaagagcttc
     8281 acgcgcgggc cgagcaggcg gcgcacgtat tcgcgcatgc cgggatcgtt ctcgacgtgc
     8341 agcacctgcg cgcgctcggc gtgcacgccg agcgcggcga tcgctgcctg caggcgctcg
     8401 ggccgcaccg gcttcggcag ccagtcggcg accggcagcg ccacggcttc cagcgagccc
     8461 tgcggcagcg ccgagacgac catgaccggc acggcgacgt ggcgcgggtc ctcgcgcatc
     8521 gcgcggatga cttcctcgcc catgccgtcg ggcagcagca tgtcgacggt gaccagcgcg
     8581 tagcggccga cggcgagttt ctcgcgcgcg gcgcgcacgc tgccggcgac ctcggcgatg
     8641 tagccgtcgc ggctcagctc ggcgacgaag gccgaggccg cgagcgcgtc atcctcgacc
     8701 acgagcacgt gcgggcgctc gctgtgcgtg ctcgtgacca cggccagcgg cagttcgagg
     8761 tgcaggcagg tgccctcggg gccggtctcg tagttgatcg tgccgccctg gcgctcggtc
     8821 agctcgcggg cgatgtacag gccgagaccg gcgccgccgg cgggccggcc ggtctcgacc
     8881 ggtccctgca ggaagcggtg gaacagccgc gggcgcagct cggccgggac gccggcaccg
     8941 cggtcgcaga cttcgatgcc ggcgcgccgg cccggcagtt tcagcgcgcg cagcgtcact
     9001 gcgctgccgg ccggggcatg gcgcagcgcg ttgtcgagca gatggccgag cacctgcgtc
     9061 aggcgctcgg ggtcggcgtt gacgctgagt tcgccggcgg cgtcgatgtc gatcctgatc
     9121 ccggccgcgg ccgcggccgc ggcgtattcg tcgcggagct gtccgagcag cgcagcgagc
     9181 gcgatcgtct gcggggccag cggcacgtcg ccggcggcga tgcgttcggc gtcctggatg
     9241 tcggcgacga tgcgcatcag ccgggcatcg tggcggcggg cgatgtcgat gagatcctgc
     9301 gcctcgccgg agaccgtgcc gagcgcgccg ctggcgatca ggtcgagcgt gccgccgatc
     9361 gtggtcagcg gcgcgcgcag ctcgcgcgcc accagcgcca cgaactgcgc gtgcagatgc
     9421 acggcctcgc gcagcgtgct cacgtcgcgg ttgatctcca gcgtcgccga cagccggccg
     9481 tcggggccgc gcagcagcac gcggcggctc tcgatcgtca gcgcgtgacc gtcgcggtga
     9541 cgctgcagca gcgtgccttc ccagtaatcg agcgcggtgc gctccggctc atcggccgcg
     9601 ccgctgacgg cgttgtgcgt gtccaggcgc tcgtgcagcg gctggccggt catctcggcc
     9661 gcgctccatc catacaggcg ctcggcgccg cggttccagt gcgtgacccg gccctcggca
     9721 tcgcggccga tgatcgcgtc gtgcgcgcgc tcgatcagcg cggcctgctc ggtgcgtgcg
     9781 cgctcggcct gacgctgggc gtcgatgtcc tcgatctggg cgatgaagcc ggtcgggcgc
     9841 tgctggccgt cgcgcatgca cgaggcaacc acgcgcagcc agtgcagcga gccgtcggcg
     9901 cgcaggccgc gcagttcgta gcgggtcgaa ccgatcgtgc cctcggcgag ctgccgcgcg
     9961 agctcctgcg ccacgtagcg gtccgggccg tggatgaagt cggcgaagtg atggccgagc
    10021 agggcctgcg cctcgtggcc gccgagcgcg cagagcgccg ggttcacggc gacgaagcgg
    10081 ccttcgagat cgatcagcgc catgccgatc gccgaatcgc gcatcgcgct gcgcaggcgc
    10141 tcctcgctgg cgcgcagccg gcccatgtcg gcagagcggc attccagcag ccaggcgacg
    10201 gcgagcggtg caaactgcgc cagcgcattg atgcccggcc cgtaggtctg cagggccatc
    10261 tgcatcggga ccggaggcag taccgacagc agggcggcga acagcaggct ctcgcaggca
    10321 cacagcagcg ccgtgccgcc gaggccgagc cggaacgagg cgagcagcag cggaagttcg
    10381 atggtcacga acgggaacgg gctgagctgc aggctggcga aggtgccggc gatggtcagc
    10441 ccgtacagca ggaggatctc ggtgcgccgc ccggccgcga accagtagtg cgactcgcgc
    10501 aggctggcgc gcagcccgag cggcagcacc gcgaccatgc cggtcaggtc actggtcagc
    10561 cagttgccga tcgaggccgg cagcggccgt gcgtgcacgg tcgccagcag cgcgccgccg
    10621 acgagcgccg acagcatcgt gccggtggcg accacgagga tcagccgcac ggcgcttgtc
    10681 gggcgctcgg tcagcaggaa gggctgcgaa tcggccgggc gcagcgcgag cagcggcacc
    10741 agcacgctga ccagattcgc gaacgcatag ccggcggcca gaccgggcga gacgtcggtg
    10801 gccaggttcg cacacaggcc ggcggcggct ccggtgatca ccaccggcca gatctgccgg
    10861 tacggtgccc gcagcgcgac ggcgagcagg atggcgttcg gcagccagat cgtggcgagc
    10921 gggctgctgc cgcgcgacag caggatgcag atcacggccg ccgcgaaaca gccgagaccg
    10981 gtcagcagca tccgcaaccc cgtcggcatc ggtgacgggg gagcgtcagc cggtacggac
    11041 gtgaggggat cggtcatgag ggccacggat tctcggggcg gcggcgaggc cggtcgggag
    11101 ctggcacggt cggggtgcgg aagcgtctgc agcagcggac cgggccggaa tgcggagctg
    11161 gcatgaggcg acgcagccag ggcgggaagc ccgtttacca cagtccggcg cagcgaggga
    11221 acgccgtgaa catgacgcct tggtcagaca ggcgcttgcc agggatccac gtgttcgcgg
    11281 tcggctgcgt cgttgcgggc caccagtgcc cgggcgggtt cggtggcgct cagattgacg
    11341 gcctggtgcg gcaggtcggc ggggatgtag acgaagtcgc cggcgacgtt gacgacccgg
    11401 tgttcgagcc gccggccgta gcgggtctcg acgcggcctt cgagaatgta gatcgccgtc
    11461 tcgtagccgc gatgcaggtg cggctcggaa gccgcgcccg gcgggatcac caccagatgc
    11521 atcgccaggc cgcgggcgcc ggcggtggcc gcgcagatgc cgacgaaata cggcaggcgc
    11581 tggcgggtca ggacttcggc ctcgggccgg acggtgacga cggcggcacg gtcgccggcg
    11641 ctcgtggatg cggtttcggt catggtggcg gcctccggcg cggacatcga ccgcgagcgt
    11701 agccgctgcc ggcgcgcgcg gctgcctcag tcgccgccgg cggcggccca cagccgcgtg
    11761 gcggcggcac cggtgaggtt gcgcggccgg tagagccgga tcgacatcgg gatggtccag
    11821 gacgcatcgc cggcgtgcac cagccggccg gccgcgagat cgtcggcgac caggcgctgc
    11881 ggcacccagg tcaggccgcg cccgtcgcgc gccattgccg ccagcaccgt cgccagatgc
    11941 gcggtgaagg ccgggcgcac ctgcgccggc gcgagctgcg cctgcgcggc ggcgagcgcc
    12001 cggctcaggc ccgaggcttc ggtcggtgcc agcagcggca gcggttcgtc cggcgagccg
    12061 ggcagctcga agcgcgggcg cggatcgttg gctgcacgcg gagcgctcac cggcagcagc
    12121 acatcgttac cgaccgtgat cgagcggtaa tcgccgttgt cgaggcggcc gctgaccccg
    12181 gcgtggccgt ggcagagcag gaactgcgcc tcgccgcgca gcagcaactg ctcgcaggcg
    12241 tgcatcgtgt cggcgatcag ccggatcgag gcatcgacgg ggctgcgggc ttcgaccttg
    12301 cgcagccact gcgggaagaa gtccagcgcc agcgcatgcg tgctcgcgaa gcgcagcagc
    12361 ggcaggccgc tgtcggcggc agcgatggca tcctcgcgcc cgtgcagcag cttgcgcagc
    12421 gcagcctcgg ccgagggccg gaagcgttcg ccggctgcgg tcagcgccgc gccgtggctg
    12481 ctgcgctcga acagcggcgt gccgatccag tcctccagcg agcggatgcg gcggctgaag
    12541 gccggctgcg tgacgtgccg gctgtcggcg gcacgcgaga agctgccgca gtcgatgaca
    12601 gcgagaaaat cttcaagcca ggcgagttcc atgggcttat gtactcctga cgcaccagtg
    12661 aattcctgcg tgcagcctgc cttattgcag tgcgacattg gcatacggca aacgtcgtag
    12721 tgacaaggcc atgttttttc ggcatggcca cctgcgcgat cggcattggc tgcaaatccg
    12781 cgtatccagc gagaattcag gggtcaggcc cggtagcgct gcttgcgctg gcttgcactc
    12841 ctcgcttgcc cgtccggaga cgaacatgcc gattctgcat acggactttc tgctgcccgc
    12901 gctcgcactc ggtgtcgcac tcggctggct cggcggtctg ttcggtatcg gcggcggcct
    12961 gatcgcgatc ccggtgctcg gactcgcctg cgggctcgac cagttgcacg cgcagggcac
    13021 ggcgctggtg atggtgctgc cgaatgtcgt gatcggcttc tggcgctacc gggcgcgaca
    13081 cggcatcgcc tggcgaccgg cgctgctgct cgggctgacg gcgatcgccg gcagcgagtt
    13141 cgccgcacac ctcgccaccg gcctgccggc ggtgacgctg cgcctgatgt tcgcaggctt
    13201 cctgatcgtg ctggccggct ggctgctgcg cgggctgcgc cgcgcgccgc cggccacggt
    13261 ggcaccggca ccggccgtgc tgtcggcgcg ctggctgccg gcggtcggac tggccggcgg
    13321 gctgatgtcg ggactgttca gcgtcggcgg cggcatcgcc gcggtgccgc tgctgaccgg
    13381 gctgttcggc acgcgtcagg cggtcgcgca aggcctggcg ctggcgctgg tcacgcccgg
    13441 ctcggccgcg gcgctgttcg cctatgcccg tgccggcgcc gtcgactggc cgctcgggct
    13501 ggcgctggcc ttcggcggtg tgctgagcgt gtcggccggg gttgcattcg cgcaccggct
    13561 gccggagcgg cggctgcgtg cgctgttcgc gctcggcatc gtcgcgaccg ccggcgcgat
    13621 gctcgcatcg gcctgaagcg gtcgttcagt cgagtgctgc cgctgcgccg cgcagcgccg
    13681 catcgggggc atggatcacg aagcacggga tcggctgcag ataggccgtg aaccggccgc
    13741 gatgctcgaa gcgcgcccgg aacggcgagg cggcgaagaa ctccgggaag cgcggcacga
    13801 tgccgccgcc gatgtagacg ccgccgcgcg cgccgagcgt cagcgccaga ttgccggccg
    13861 cggtgccgag catggcgcag aacaccgtca gcacccgccg gcacagcgcg cagtcgccgc
    13921 tggtgccgcg ctgcacgatc tcgggcgccg ccatcgcctc ggccggcagg ccgtcgatct
    13981 cggcgagcgc ctgatgcagg ttcggcaggc cgagcgcgcc ggagaccagc cgctcgaccg
    14041 atacgtgcgg gtggcgggcg agcgcccagc gcaggatttc aagctccagc gcatcggccg
    14101 ggctgaagct cacgtgcccg ccctcgccct cgatcgcgcg ccagccgccg tgcccgtccg
    14161 gcagcagtcc ggacaccccg agcccggtgc cggggccgat cagcccgatc gccgtgcccg
    14221 cgaccggcac cgtgccgccg acctgctgca gcgcctcacc ggcgagcagc ggcagcgaca
    14281 gcgccagcgc cttgaagtcg ttcagcagtt cgagccgctc aagaccgagc gccgtgcgcg
    14341 tcgcctcgcg cgagaagcgc caggcattgt tggtcatctc gacgcgatcg ccggtgaccg
    14401 gattggcgat cgccaccgcc gcccgctgcg gtcgcggccc gccggcgtgc cgcagatagg
    14461 cgtcggccgc cccggcgaga tcggcatagg ccgaggtcgc gagcacctgc acggcgcgcg
    14521 gcgtgctgcc cgggccgtcg ctgagcgcga aacgggcatt ggtgccgccg atgtcggcga
    14581 tcaggtcggg cgtgcggtcg gaagcgctcg aagacatggg caacggactc ggcagcgggg
    14641 agggaagaaa ggaaggggag gagcgaaggg attgccggat accgacgccc tgcgggcgcc
    14701 ggtgttccgc gtccggcgtt cagccggccg ctgccagcag cccgcgctcg gcgagcacgc
    14761 gctcgacggt atcgacgatg gcctgggtct gggcgtcgat ctcgaggttc acgagtgcgc
    14821 cgacctgcag ttcgccgtag gtggtggcgc gcagggtttc cggaatcagc cagacatcga
    14881 aggtgtcgcc ctcgacgcgg ccgatggtca ggctgcagcc gttcagcgcc acgaagccct
    14941 tcggcagcag gtacttcatc agcgccgccg gcgcccggaa cacgacttcg tgattgttct
    15001 cgggccgctc gatgcgaatg atttcggcga gtgcatgcac atggcccgac agcacgtggc
    15061 cgccgatctc gtcgccgtgg cgcgccgcgc gctcgacgtt gacgcgatcc ccgaccgcga
    15121 gccggccgag gttggtcacg cgcagcgtct ccagcatcgc atcgaaatgc acgagccggc
    15181 cctcgaagcc ggtgatggtc aggcaggtgc cgttgaccgc gacgctggag ccgatcgcaa
    15241 gcccggtgca cagccgctcg ggcagctcca gcgtcagggt gacgagttgc ggacgctcga
    15301 cgagcgcgac gacgggcacg gcggcctgga cgatgccggt gaacatggcg atacctccgg
    15361 aggacggggc ggacggcgct gcggacccgt gcgtgccggt cgcacggggg cgctgcaccg
    15421 caacaggaag cgggtcctgc ggatggtaac gcgggcgggg tgtcggcagc cgctgccgat
    15481 gaggcccgga gcagacgttc agccgttgct gaccttcccg cccggcgccc cggcatccag
    15541 gctgcgcacc ggcggcagcg tgcggtccgg accaccggtc ggcaccggtg gcggcgcaac
    15601 gccggcgagc aggccggcga cggcgagggc gtcgcggatc atgtccatgg ccttgtcgag
    15661 cacgcggcga cggccgaaca cgctccaggc gctgttcagc gccaggcgcc cgaggacgga
    15721 tctgacgttc tccttcagca gcgccccgac cacggcatcg gcgcgggtct tcaggccgcc
    15781 ctgcaccggt tgcagctcgg attcgcggat ctgcggccac ggcaggccga acggagcctg
    15841 cggctggaag ccgtcgatcg ccgctccggc ggcgctgccg aacagcggca ggatcgggac
    15901 cagctgcacc ggggcaccgt cgtagaggtc gggatcggcg ggtttgcggc cgaaccgctt
    15961 gtcgtagtcc gaatccggac cggccgtgcc gccgaacagc gggttgtcgc cggcgagcac
    16021 gaagtgccgg cgcaggaact gctggcagtt caggcgcccg agcaggtagt cgtgggcgcg
    16081 gaaggcgcgg ttcaggaagc cgccgaagcc gccgagcgtg ccgcacgcga tcggcgtgcc
    16141 gggcagctgc acgcgcctgc cgtcggctgc gtagcgcacc ggcgtgatca ggaagcggct
    16201 gtagacatcc ggatgatcgg cgagcatcag ctcgggcagc ttgaagcgca tctgctggat
    16261 cgcggcgctg agcaggtgct tgatcgccgg gatcagcttc aggccctcgg gcacctggta
    16321 gtcctcggtg aacaccggcg ggcacgggaa cgggtcgatc atcagcaccg cgcagtcggc
    16381 ctcgtgcggg ctgcgcgggt tgtgttcgcg gccatggtct tcggcgagcg tgcgccggcc
    16441 gatctcgaag ggctcgttgt cgatggtgcc gccatcgacg ttgacgtagt cgtaggcgcg
    16501 cgtgcagacc gtggccggca gcgcggtcgg gattctggcc tgtacggtgc agcgggcaat
    16561 gacatcgggt cgggccgcat ggaactcggg cagcgtccac agccgctggt tgtagatctc
    16621 ggcctgcgtg cgcttgagcg cccgcggcgc gaggccgacc gggaaggcgc cgctcgcgag
    16681 cgcggcctgg ccgagctggc cccagccgcc gccggtgccg ttctggcggc cgagcacgat
    16741 ggtctcgccg tcctcgctgg ccgtgctgcc ggggtcgagc cggaagcgga tgctgtcggc
    16801 gtgcagcgtc atgccgtaga ccgcctgcgc ggcgtcgccg cggaaatcga tgccgtaggg
    16861 cacgccgcgc tggttgttga gcgtgaagta cagatcgagc ggctggctga cgaaggccgg
    16921 cagcgacgca gcccctgcgg cccagtcgac ggcgagcgcc tgcgcggcga tgcgatcgag
    16981 cgcggtcgaa tcgagcagcg aactgagcgg cccctgcctg aggtcggcgg tgccgagcag
    17041 gccggaaatg tcgacgcgct cgacccaggc gtcgaacagc gggttgctgg cgccggctgc
    17101 cgggtgctgc gtgcgcaccg gcgtcacgcc cgggcgcagc gtcgaggcga agatcgcgcc
    17161 gacgatggag ccggccgaag cgccgctgat cacgcggatg cgcacgcgcc agggatacct
    17221 gcccggctgc ctggcctttt cggcctcgaa ggcggtcagc gcctcgacca ggaaatccag
    17281 caccccggcc gtgtaggcgc cggccgacac cgcgccggcc atggtcagcg cgatgtcgaa
    17341 aaccggctca ttgttgttgt cgcccatgtt ccgtcctccc gcggtgcgtg cccccgtggc
    17401 acgtgcggtc aggacatagc agaggcgtgc aacacctggc gcaatgcgca ggtgaacatc
    17461 cggaaaggat gccggcgggc gcccgggcgg atggcggcgg gctgaaacgg caccggcccg
    17521 tccggggagg ggccggtgag ggcgccgcgg cgcatcagtc gccggattcg gcggcgcgcc
    17581 gggcgtcttc ctccatcttc ctgcgcaggc tcagcgggcg catgtcggtc cagacctgct
    17641 cgatgtgggc aaggcaggtc tgcttgttgc cctgcgtgcc ttcatgcgtc cagcccaggg
    17701 gcagctcccg gtcggcgggc cagatcgagt actgctcctc gtggttgacg accacggcat
    17761 agagggtggt gtcttcctgt tcatcgtaac tgctcatcgt cggactcccg tgccggttca
    17821 ccgccgggtt tgcggtgctc cgggagtctg tcggcaagca gccgccgctg ccggcaagcg
    17881 cggctttcgt gacggagtca ggattttcag ttcgtcaggg agtcgcggcc ggtgtgggca
    17941 gggcgggaac gacgccggcc ggccggcagt gcagggggct gtacgtcatc ggctgccggt
    18001 acgtcatccc cgcctgcatc cggggcggct gcgaaaaccg cgggagcggc gtgcgcctgc
    18061 acgcaggcca tgcattccga gcgcgtgcct tcggtgcctt cgagccgcca gcccggcggc
    18121 ggacagcgtt cggcgcgaca gagtgaatat tcgccgttgc tgccgatcat cacggcatag
    18181 cggatcgcgg tatcgacgac gacgcccatg gctgtcctcc tgcaggccgg gtccggatgc
    18241 gcggggttgc gaccggtacc tgctggcctt ttgccagcaa atgatgcact gcggcaagcc
    18301 cggattttgt gcgggaatcc gggcaggagt tccccggaaa ttccacgcag gccgtgcttg
    18361 ccggcctcag caggccgcgg cggcggcggc cagtgccagc agcccgtcgg tctgcgcgac
    18421 gatcgccgcg tgcacggacg gatcgagctt cttcagccag cgccgcggca ggcggctggc
    18481 gccgcagcgg gcgccggcga gctggccgac gatcgcgccg gtggtgtcgg catcgccgcc
    18541 gcggttgacg gtgcggatca ggcaggcctc gaagtcctcc tcggcgaaga aggcatcgaa
    18601 caccgtctgc acggtatcga cgatgtagcc gccggcctgg cccggatagg gctcgaagcg
    18661 gaactgccgg tgcgacgcga tcagcgtgtc ggcctggagg cggcagtcgg cggtcgtgcc
    18721 gccgagcatc agcgtctgtg tcatctgccc gagcgtgacg gtggcggcgt cggagagcgg
    18781 gttgtgatgc gtgaagcgcg cctgttccag cgaacgcttg accagcgccc tcgggtggcc
    18841 gagcgtgacg atggtcaccg gcacgttgcg catcgccgca ccgttgccgg cgtcggcgct
    18901 ctccggagtc agcagcgagc cgtccttcag gtagcgccgg atgccgcgcc ggcaggtgtt
    18961 gccgacatcg gccggcttgc tgcgcagcca ctcggcccag gcttcggcga tcgccgtcag
    19021 ctgccagccg ccggactcga tcagcgccag cccgagcgcc atcgacatct gcgtgtcgtc
    19081 ggtgacggcg ccggccctga gcttcagcca gccgccgccc ttgatctcgt cgtgccggcc
    19141 gtgctgcgcg gcgatttcgc gcgcggtcag gaattccacg gttgcaccga gcgcatcgcc
    19201 gagcgccagg ccgaggtagg cgcccagggc gcggtcgcga acgtcactgt cgtacatcgc
    19261 agggctccgg gcatgcggca tcagagccag ctggtgcgca cgcggtagtc gccgccgatg
    19321 acgaggtact cgccctcgcc cttgagcatg tgcttcggca gcaggtcgtt gaagaacagg
    19381 atcttggtca gcggcacctc ggcttcgagg atgcagtcgc cgaactcgcc ggcgcggtcg
    19441 cgatcggcgc tgaaggaact gaggttgttc aggcgcagct gcgccgtgcg cttgtcgatg
    19501 cgctgcacca gcgggtgctc ggtgagatcg ttgacgccgc ggtacagccg caggtgccgg
    19561 gccgggggcg gcagcagcgg gaaatgttcg agccgccact ggcagaagct gtacagcagg
    19621 tcgagctgcg cgtgcaccgc gttgttgtgg aagcgcggcg ccatcttctc ctcgacgtag
    19681 gcgatccact gcgggctgga gaagcgcgtc agcgccgcct tgtgccaggt cgggaacagg
    19741 ccgaagcggc tctcgaccca gcccttgagc accgcgccct cggggttgct ggcgtcgaag
    19801 gcccagccct tgagcaggcg caggtagctc gcgcgaaagc gccggcaggc gccgcaggcc
    19861 ggttcgtccg ggcgggcgag accgaacacc acgtccatgt agtcctggaa gatctgcccg
    19921 gccgagcgcg cgtccgggca ttcggcgagc ttcgagtaca gcgcccggtt ggtctcgcgc
    19981 acggccggga tcaccagcgg caccggggcg tcgttgtacg caaggctcgc gatcaccgcc
    20041 gccggcacgc cgaccagcgg cgtgctgtgg ccgatgcgcg acaacggtgt cggttcgggg
    20101 gtgtgcggcg tgttttcggt gccggtccgg gtcaggctca tcaggcaact cctcctcgtt
    20161 cctcgttcct gctcgttctt gtcgtggtcc cggcgcctgc gctgcggccg gcatcgccgg
    20221 tcctgtcacg gacccggccg ctgccgggct gcgccctgcc ggttttcgtg tcggctgtct
    20281 gtcggctgtc gtgtcggtgt tcatgtcggc attcatgtcg gctttgtcgt gccggctgcc
    20341 gccggctgca gcccaaagcc gacaaatcgc cgatccggga aaccgtgagc aatccacatg
    20401 ccggagcacg cgcgcgcgta cgcgcgtgcg atcggctaat tcagggcatc ggccgggatt
    20461 gatccagatc aattcattgc cggtcattca ggaaacggga cgggtagccg gcggcctgca
    20521 cgcggtctgt cggcattcgg ccgaatgtcc ggatttgtcg gagaggacac acggtgcgac
    20581 agtcatgcag ccttgtgtcc ggagtctttt tatccatttt caatcaatga cttgtattat
    20641 tttatcgagt ttggcacgcc ggttgctcat tagctcgtgg ggagcagcaa ggtcccgatg
    20701 gttaacaaaa tcctgaaacc tgatctcgaa ggaagacggc aaatgagcac gcttaggcag
    20761 attgccttct acggcaaggg tggaatcggc aagtccacca cctcccagaa caccctcgcc
    20821 gcgctcgcgg aaatgggtca gaagatcctg atcgtcggct gcgacccgaa ggctgactcc
    20881 acccgcctga tcctgcacgc aaaggcccag gacacggtgc tctcgctcgc cgccgaagcc
    20941 ggcagcgtcg aggatctgga aatcgaagac gtcatgaaga tcggtttccg cgacatccgc
    21001 tgcgtcgagt cgggtggtcc ggagccgggc gtcggctgcg ccggtcgcgg tgtcatcacc
    21061 tcgatcaact tcctggaaga gaacggcgcg tatgacggcg ttgattacgt ctcctacgac
    21121 gtgctcggcg acgtggtctg cggcggtttc gcgatgccca tccgcgagaa caaggcccag
    21181 gaaatctaca tcgtcatgtc cggcgaaatg atggccatgt acgcggccaa caacatttcc
    21241 aagggcatcc tgaagtacgc gtcctcgggc ggcgtgcgcc tcggcggcct gatctgcaac
    21301 gagcgcaaga ccgacaagga actggaactg gccgaagcgc tggccggcaa gctcggcacc
    21361 aagctcattc acttcgtgcc gcgtgacaac gtcgtgcagc acgccgagct gcgtcgcatg
    21421 accgtcatcg aatacgaccc gaactgcaag caggccggcg agtatcgtca gctggcggaa
    21481 aaggttcaca acaacgccgg caagggcgtc attccgaccc cgatcacgat ggacgaactc
    21541 gaagacatgc tcatggagtt cggcatcatg acgaaggaag acgagtccat cgtcggcaag
    21601 gcggcgtcgg cggcctgatc ggcgccgcga gccctccctc cgaaaatgcg ggggagggcc
    21661 gggacggggg taaaccacac gcggcggcat cccgttccgt cccgcccatc ggcgaatggg
    21721 tttgccgcat cgtaaacagg attccgattc tgggagggcc agatgagctc gacggctgaa
    21781 gacatcaagg cgcgcaacaa ggcgctgatc gcggaagttc tcgaagttta tcccgagaag
    21841 gcgaagaaga agcgccagaa gcatctgaac gcgtatgaag gcggcaagcc ggattgcggc
    21901 gtcaagtcca acatcaagtc gctgcccggt gtcatgacca tccgtggttg cgcgtacgcc
    21961 ggttccaagg gcgtggtgtg gggtccgatc aaggacatgg tccacatctc gcacggtccg
    22021 gtcggctgcg gccagtactc ctgggcctcg cgccgtaact acttcatcgg cacgaccggc
    22081 atcgattcct tcggcacgat gcagttcacg tccgatttcc aggaaaagga catcgtcttc
    22141 ggcggtgaca agaagctcga aaagatcatc gacgagattc aggtcctgtt cccgctgaac
    22201 aagggcatct cgatccagtc cgaatgcccg atcggcctga tcggcgacga catcgaagcc
    22261 gtcgcgaaga agaagtccaa ggaatacgaa ggcaagacca tcgttccggt gcgttgcgag
    22321 ggcttccgcg gcgtttcgca gtcgctcggc caccacatcg ccaacgacgc gatccgtgac
    22381 tgggtgttcg acaagtccac cggcgagatc gaaggcttcg tgccgacgcc gtacgacgtg
    22441 acgatcatcg gcgactacaa catcggcggc gacgcctggt cctcgcgcat cctgctcgaa
    22501 gagatgggcc tgcgcgtgat cgcccagtgg tccggtgacg gcacgctggc cgagctcgag
    22561 aagaccccga aggcgaagct gaacctcatc cactgctacc gctcgatgaa ttacatctcg
    22621 cggcacatgg aagagaagta caacattccc tggctcgaat acaacttctt cggcccgacc
    22681 aagatcgagg aatcgctgcg tgccatcgcc gcgcatttcg acgacacgat caaggccaat
    22741 gccgagcgcg tgatcgccaa gtacaaggcc ctgacggatg ccgtcatcgc caagtaccgt
    22801 ccgcgcctcg aaggcaagaa ggtcatgctg ttcgtcggcg gcctgcgccc gcgtcacgtg
    22861 atcggtgcct acgaagatct gggcatggaa gtggtcggca cgggttatga gttcgcccac
    22921 aacgacgact accagcgcac cacgcattac gtgaaggacg gcacgctgat ttacgacgac
    22981 gtcaccggct tcgagttcga gaagttcgtc gagaaggtcc agcccgacct ggtcggttcc
    23041 ggcatcaagg aaaagtacgt gttccagaag atgggcgtgc cgttccgtca gatgcactcc
    23101 tgggactact ccggtccgta tcacggctac gacggcttcg ccatcttcgc ccgtgacatg
    23161 gacatggcga tcaactcgcc ggtctggggc ctgaccaagg cgccgtggaa gaagtgatta
    23221 gcgtgcgggg cggcaagccg acatacgccc cgcctttacc cgaattcatg tgattttgga
    23281 gacaggccat gagccagaac gctgaaaagg tgctggacca cttcaagctg ttccgcgagc
    23341 cggaatatgt ggagatgttc gagtccaaga agaagaactt cgaggacgcg gtgccggcgg
    23401 aagaacttga gcgcgtccgc gagtgggcga agagctggga ataccgcgaa aagaatttcg
    23461 cccgcgaagc cctgacggtg aatccggcca aggcctgtca gccgctcggt gcggtgttcg
    23521 ccgcggtcgg cttcgagagc acgctgccgt ttgtgcacgg ctcgcagggt tgcgtcgcgt
    23581 actaccgctc gcacttcagc cgtcacttca aggagccgac cagctgcgtc tcctcgtcga
    23641 tgaccgaaga cgccgcggtg ttcggcggcc tgcagaacat gatcgacggc ctcgcgaaca
    23701 gcaacgcgat gtacaagccg aagatgatcg ccgtgtcgac cacctgcatg gcggaagtca
    23761 tcggtgacga cctcaacgcc ttcatcaaga ccgccaagga aaagggctcg gtgccgatgg
    23821 acatggatgt gccgtatgcg cacaccccgg ccttcgtcgg cagccacgtg accggctacg
    23881 acaactcgat gaagggcgtg ctgacgcact tctgggataa cgccgagcgc accccgaacg
    23941 agtcgatcaa cctcatcaac ggtttcgacg gctacgtcgt cggcaacatc cgcgagctca
    24001 agcgcgtgct cggcctgatg ggcgtggact acacgctgat cggtgaccag agcgacgtgt
    24061 gggatacgcc gaccgatggc gaattccgca tgtacgacgg cggcaccaag atcgaggacg
    24121 tgaaggctgc ggtcaacgcc aaggccacgc tgtcgatgca ggaatactgc accgagaaga
    24181 cgctcgccta ctgcgccgac aagggccagc agacggtcgc gctgaatcac ccggtcggca
    24241 tcgccggcac cgatcgcctg ctgatggcgc tctccgagct gaccggcaag gagatcccgg
    24301 cggagctgct gaaggagcgc ggccgtctgg tcgacgcgat cgccgactcg caggcgcaca
    24361 tccacggcaa gagcttcgcg atctacggcg atccggacct ctgctacggc ctgaccgagt
    24421 tcctgctcga actcggcgcc gagccgaagc acgtgctctc gaccaacggc aacaaggcct
    24481 gggccgagaa gatgcaggcg ctgttcaaca gctcgccgtt cggcaagggc tgcgtcgcct
    24541 acgccggcaa ggacctctgg cacatgcgct cgctgctggc gacggagccg gtcgacttcc
    24601 tgatcggcaa cacctacggc aagtacctgg agcgtgacat cggcaccccg ctgatccgca
    24661 tcggcttccc ggtgttcgac cgtcaccacc accaccgctc gccgatctgg ggctatcagg
    24721 gtggcatgaa cctgctcgtc aagctgctcg acaagatctt cgacgagatg gacaaggaca
    24781 ccaacatccc gtccaagacc gattacagct tcgacatcat ccgctgaggc tgcccggacg
    24841 caccgcgcgt gcgtgaaacc cgccccgagc ccggggcggg cgggggaacc ggaggcgccg
    24901 gctccgaccc ccactcccgg cggccccctg agtgcgcttt ctgcggagag ccggggccgc
    24961 ctctctattt cgggcgggtg ccgttgctga aggccggcga ccgtgaaggc ttccgtcgtt
    25021 acatctggag aatcaggatc atggccaacg ttacgttcag ttcgccgagc atgaagaagg
    25081 atgtcaccgt ctacgcgacg gccggcgaca cgcagacgct gctggcggtc gccaagcagc
    25141 acaagatccc ggtgcacttc gagtgcgaga acggcgagtg cggttcctgc gtcgtgcagg
    25201 tcacggtgct gaccgccaac gccccgtacg cgatcgcgct gacggagaag gaaaaggcgg
    25261 tcctgaagct cgccggcaag atcacgccgg agcagatcga caacgccgag gtcaacgacg
    25321 tgccgccgcc ctaccgcctg gcctgccagt tcatcgtgcg caacgaggac atcctcgtga
    25381 agttctgatt gcggccggca cccggtgacg ggtgctgcca gggaagcgcc tcgacccgcc
    25441 gcctgcacgg cgggtcggtt tttttcggtt ccgtgaccgc caggcgacgg tcgtccgtgc
    25501 gggcagcgcc aaccttaccg ttctgtcatc agcgtttcat gctgcgttgc cacactgcgc
    25561 cgccttgttt cgaacggcag agcaggagga cagccgccgt gagcgcagcc gacaccgcaa
    25621 ccgaactatc gctggcgctc tgggcgctga agcgggcact cgcgcgccag gatgcccatg
    25681 cccagcccga catcgaccgg ctgcgtgtcg atgccgccta ccgcgacacc atcgtcgccg
    25741 aagccggaca aagcccgcac gaggccgtgc gcgaggctgc ggcgcgcgtg cgcgccctga
    25801 tcagcgccgc cgtgcaggaa gcgaggcagg acaaggccgc gcgccgcggt gcgcgcacgg
    25861 cgctggtcgc cggcatggtg ctggtgctcg cggccgccgg cgtgctcggc tggcagtggc
    25921 agacgcttca gccccggcag gggcgcacac cggttgtggc gagtgtcgcg ccgcagccgg
    25981 ctgcagtcgt gactcccgca gcgattccgg cagccgtgac ggtgccgaag ccggtgacgc
    26041 aggcggctgc gccgctcgca gcggaagcgg cgaacacccc ggtccagctc ctgtcccggc
    26101 cggaggacat cgcgccggcc gcggccggga gcgtaccggc agccagcgcc gcgctcccgg
    26161 tggtgccggt cgcagcgctg ccggcagcgg ctcccgtgaa gctgcgcatc catggctcga
    26221 acacgatcgg ggccgaactc gcgccggcgc tggtcgagcg tttcctcgcc cggcgcggcg
    26281 cgagcgacgt gcgccgcgtg ccgggcgccc gggccgagga aatgcgcatc gaggcgacgc
    26341 tgcccggcag cgaggggcgg atcgcgatcg aactggccgc gcacggcagc ggcacggcct
    26401 tcaccgatct cgccgatggc cgggccgacg tcggtgccgc atcgcggccg gtccgggatg
    26461 aggaaatccg gaagctcgcc gcgctcgggg atttctcgtc gccggcggcc gagcacgtca
    26521 tcggcctcga cggcgtcgcg atcatcgtcc atccggccaa tccgctgcgc tcgctgagcc
    26581 gcgagcaggt cgcgaagctc ttcgccggcg agatccgcga ctgggccgaa ctcggcggca
    26641 ggccgggtcc ggtgcacatc tatgcgcggg acgagcgttc cggcacctgg gacaccttcc
    26701 agtcgctggt gctcggcaag gcctacccgc tcagtcccga ggcacagcgc ttcgaatcca
    26761 gccccgagct gtcggatcgg gtcgcgaccg acgccggcgg catcggcttc atcggtctgc
    26821 cctacgtgcg catggcgcgg gcgctggcga tcggcgatgg caatgccgtg ccgagcgcgc
    26881 cgagtccgtt cacggtcgcg accgaggatt acccgctgtc ccgccgcctg ttcttctatc
    26941 tggcgccgac cggcgacaac cgcatggccc gcgaactggt cgagttcgcg ctcgagaacg
    27001 acggtcagca ggtggtcgag gcccacggct tcatctcgca gcgcgtcgcc ccgcagcgcg
    27061 tcgcgctgtc gccgaacgcc ccgaaggagt acgagtcact gacgcgcgat gcgctgcgcc
    27121 tgtcggtgaa cttccgcttc cgccccggca gcggactgct cgacggcaag gcgcagcgcg
    27181 acctgcgccg ggtcacggac tacctcgcgc aggccgagaa ccgcagccgc caggtgctgc
    27241 tgttcggctt caccgatgcc cgtggcagtc cggagcagaa cctcaggctg tcgcagcagc
    27301 gcgcgcagga gattgcccgc gaactgcagg cccgcggcat ccagacccgg gtcgtgcgcg
    27361 gcttcggacc ggccaacccg gtcgcggccg acgattcgcc gagcggacag gagcgcaacc
    27421 gccgcgtcga gatctgggtg atgtagtgct cagggcgccg ggcgcagcag gcgcggcggc
    27481 acgccggcgc cgcgcttgcc gttggcgagg cgcacgtcgt attcgaagcc gccgcgccgg
    27541 gccggacgca cggcctcgat gcgcgccggc agccagtcgc tgaggtattt cgcttcgacc
    27601 gcctgaccga cgcggtactc gggcgccggt gccggcgccg ggaaaaacgc atcgaagccg
    27661 ccgcttccgt ggccgccgcc gttggccggc ggcggttcgc cggttccggc cccgggctgc
    27721 ggtgccggaa ccggcgcacg gccgccggcc gaacccggcc tggtgccggt gcgcgggcag
    27781 agcacctccc actgctgggc aatgccgagt ccgccgctgg cgttgtcctg accgacgatg
    27841 cggccctgcg cgtcgcgcca gcgcgtgcgg ccgcgcccga catcgctgcg ccagacgccg
    27901 gcgaaggtgt aggtctcgcc atcgaccggc gtgatgcgcc cggccgtgca gtcggcctcg
    27961 gcacgccaga tgcgcttcgc atcgtcgccg gccatctgtt cggccacgca ggcggcgacc
    28021 ggccggccgg gctcccagtt ttcgcaccag ccggcggcgg cctcgcgcgt gatgcgcgcc
    28081 tcggcgacgg cgtgtgcggt atcgagcccc tgcgtgcgga acacggtcgg attcaggcac
    28141 ttcgcgcaga gcgccagcca tggtccggcg aaggccggca gggccgtgcc ggaaacgagg
    28201 acgagcaggg cgaggcaatg gcggcttcgg tatgacatgc ggcagtggcc ctcccgggga
    28261 gcggtggcgc atccctgccg gatcggacga tcagcgccgc tgcaggaagc gcaggatctc
    28321 ggtcgcgaag gcctcggcgc gccaggtgtt ggtcgcggtc acgtgcgtcg cgtgcggcag
    28381 cagccacagc ccgcagtccg ggatgtggtc gtacatcgcc agcacgtcgt ccatcggcac
    28441 cagcggatcg cggtcgccgc agaccaccag cgtcgggcag cgcagcgcgc gcagcgtctc
    28501 gatcgtgtgc gcgtgatcgg aggtggccgg gtcgagggcc tccgagacgc gcgtgatcac
    28561 ctgctcccag gcatccgggc cgccctgcgg ctggtggatg cgcgacagcc agccggcgag
    28621 gccgacgccg cgccagtagg cggcatcggc catctcgcgc gtgccgccgt gcgtgtgctc
    28681 gttcttgtgg tagctgacgc ggtacagcac cagcttcgcg acccgcgccg ggtgatgcac
    28741 ggccagccag agcgcggtcg caccgcccat cgacgagccg accagatgcg cgcgctcgat
    28801 gccgagcgca tcgagcgtgt cgagcacgcc gcggccgcag tcgccgatcg acaggtgcgc
    28861 ggcgcgcatc gggctcgcgc cgtgaccggg caggtcgggg gcgatggtgc ggtggtgctt
    28921 cgcgaaggcc ggcagctgcg gcttccattc ctcgtggcac atgccgccgc tgtgcagcag
    28981 cacgagtgcc tcgccgctgc cggcttcgat gtgatgcagg gctggcatcg ttagcgtctc
    29041 cgcgtcgccg caaggacggg cattgtatgc tctgcaggca gacgactttc catcctgagg
    29101 atcacccgcc atgcgtaccg gatcgatccg ggccctgagc ccgcgcggct tccataccgt
    29161 gcgttacgtc gagtggggcg aggccgacaa ccccgaagtg ctgttctgcg tgcacggcct
    29221 gacgcgcaac ggccgcgatt tcgaccgtct ggcgcgtgcg ctcgcggccg attaccgcgt
    29281 gatctgtccg gacgtggtcg ggcgcggtgc cagcgactgg ctcgccaaca agctcgacta
    29341 cggctacccg cagtacctga ccgatctggt cgcgctgatc gcgcgcagcg gcgccgaaca
    29401 ggtcgactgg ctcggcacct cgatgggcgg gctgatcggc ctgatgctgg ccgcgcagcc
    29461 gaatcacccg atccgccggc tggtgctgaa cgacgtcggt ccgctgatcc cgaaggaatc
    29521 gctcgaacgc atcgccgcct acgtcggcgc gccgcaggtg ttcgacacgc gcgaggactt
    29581 cgaattgtac gcgcgcgcga tctgggcacc gttcggactg accagcgacg gcgaatggca
    29641 ggacctgctc gcgcactccg gccggcgcac cggggacggg cgctgggcct tcaactacga
    29701 ccccggcatc gccgagccct tccggcagat ggcgatggcc gacatcgacc tctggccgct
    29761 gtgggaccgc atccgctgcc cgacgctgct gctgcgcggc gagacctccg acgtgctgcc
    29821 gcgctcggtc gcgcgcgaga tgacgacgcg cggcccgaag gcgcggctgg tcgagttccc
    29881 gaactgcggc cacgcgccgg cgctgatgaa tgccgagcag atcgccaccg tgcgcgactg
    29941 gctcacgacg gcggagacgg cctgatgccc ggcagcccgg ccccggggcc cgcgaagggg
    30001 cgcgcttgat cgagctgctg ctgcgcatcg gcggcatcgt ctttccggtg ttcgcgatcg
    30061 cggcggtcgg cgcgctgtgg gggcgccggc accggccgga catgagcgtc gtcaaccggc
    30121 tcaacgtcga ggtgttcgcg ccggcgctgg tgttctgggc gctggccgac aagccgctcg
    30181 actttgatct gtatcgcgac ctcgccatcg gcggcatcgc ggtcgtgctc ggttccgggc
    30241 tgctgctgtg gccgttcgtg cgcctggccg gcatcgacgc gcgtaccttc attccgccga
    30301 tgatgttcaa caacagcggc aacatgggca tcccgctggc gctgttcgcg ttcggcgaga
    30361 cggcgctgca ggcggcggtc gtgctgttca tcatcgaaat ggtgctgcac ttcacggtcg
    30421 gcctgtacat cctcgatcac cgcacgcgac cgtggcggct gctgaagatg ccgatgatcc
    30481 aggcgacgat cgccgggctc gcctgcagcg ccttcggcct gacgctgccg gcgccgctcg
    30541 ccaacaccat caagctgatg ggccaggtgt cgatcccgct gctgctgttc gcactcggcg
    30601 tgcgcctgct cgacgtgaac ctgcgcgact ggaagctcgg cacggccggg gccttcgccg
    30661 gaccgctggc cggggtcgcg agcctgtggc tggtgctgcc gtggctggcg ctcgacgagg
    30721 cgcagttccc gcagctgctg atcttcgcgg cgctgccgcc ggcggtgctg aacgtgctgg
    30781 tggccgagca gtaccgccag gagcccgagc gcgtcgcctc gatcgtgctg atcggcaacc
    30841 tcggcagcct cgtggtgctg ccggccgcgc tatggtttgc attgccacac tgaaacgcag
    30901 tccggcggcg agtcctgccg tccggccccg tccccccgca ggaggcttcc gccatgaccc
    30961 agcgacagca caacgtgctc ggcgaaccgc tggccgtctg ttcggtgctg ccgatgaccg
    31021 gcttcacgcg caacggctgc tgcgatgccc attccggtga caccggcagc cacaccgtct
    31081 gcgtgcgcgt gaccgccgag ttcctgcgct tctcgcgcgc atgcggcaac gacctgtcga
    31141 cgccgctgcc ggcctggggt tttccgggcc tcaggcccgg cgaccgctgg tgtctgtgcg
    31201 ccgcccgctg gcaggaggcg ctcgaagccg gcgtcgcgcc gccggtgctg ctcgccgcca
    31261 cgcatgcgcg cgcgctcgaa cacgtgcgcc tcgacgacct gcgccggcac gcactcgatc
    31321 cggcctgagc cgccggcagg catcatcctg cacggccagc acgccgcccc gtgcggggtc
    31381 tcgccggcgt tctgccgatc ccgcagaaga tccttccggt tctttttgcc agcccggcca
    31441 gttctcacaa cattgccaat tttgccaaca cggagtcgca gcgcatgcac acgtccctgg
    31501 tgctcacggt gatcggcccg gaccggcccg gtctggtcga ggcgatcgcc gccgtcgtcg
    31561 cccgccacgg cgcgaactgg gaagaaagcc gcatggcgca gctcggcggc cagttcgccg
    31621 gcatgctgcg cgtgaccgcg cccgaggaaa cgcaggatgc cctcgaggat gcgctcgccg
    31681 gactcggcag cgtcggcctc aaggtcaccg tcgcgcgcgc cgccggcgcc caggcgcagg
    31741 tgccgaagct gctgcacctg aacctggtcg gtcaggaccg ccccggcatc gtgcgcgaga
    31801 tcgccgaggc gctggcccgg cgcggcgtgt cgatcgagag tctcgaaacc cgctgcgtca
    31861 gtgcctcgat gtccggcgag atgctgttcg aggccgaggc cgtgctcgcc gtgccgccgg
    31921 cgctcgccgc cgacgaactg cgcacggcgc tcgaagccct ggccaacgaa ctgatggtcg
    31981 atctcgatct cgccgaaccg gccggtgcct gagcccggat tgccggactg cgcaagtttc
    32041 tgaatcgccg atcgattcat gtcaggccgc tctggaggca gggtcgtgca acgctacctg
    32101 attcctttgt aattcagagc attgtcaaga
//
LOCUS       DAOQIE010000006        31408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_611_length_31408_cov_7.964820, whole genome shotgun sequence.
ACCESSION   DAOQIE010000006 DAOQIE010000000
VERSION     DAOQIE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 31408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31408
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_31408_cov_7.964820"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="thiE"
                     /locus_tag="PK144_00745"
     CDS             complement(<1..572)
                     /gene="thiE"
                     /locus_tag="PK144_00745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNF64280.1"
                     /translation="MTERPRPHGLYAITDPGLIPDGQLLERVSAAIDGGARTIQYRDK
                     RADAALRRQLAAALAGLCRARAVTLIINDDVALAAETGADGVHLGRDDAAFAAARRRL
                     GARALIGVSCYDSLERARAACAAGADYVAFGAFYPSAIKPDAVRAPLSLLGEARHALA
                     RRIPIVAIGGIDAGNGAGLVEAGADALAVN"
     gene            complement(565..1374)
                     /locus_tag="PK144_00750"
     CDS             complement(565..1374)
                     /locus_tag="PK144_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNF64281.1"
                     /translation="MSQSYTVPVVMTLAGNDPSGGAGLQADIEAIASMGCHAAPVVTA
                     LTVQDTCDVLAVVPIDPDLVYAQARTVLNDAPVAAIKIGLLGSPANAEIIAELLEDYP
                     HIPLILDPVLAAGGGRDFDGDALADAIRRHLLARTTVLTPNSVEARRLAPAADTLDAC
                     AQALLDTGCAHVLITGTHETTDAVVNRLYGDGRLLDSWTWERLPHDFHGSGCTLAAAL
                     AGLLAQGNELHAAVAQAQEYTWEALRGGYRIGAGQRIPNRLFWADEDGADD"
     gene            1495..1665
                     /locus_tag="PK144_00755"
     CDS             1495..1665
                     /locus_tag="PK144_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNF64282.1"
                     /translation="MKQYMCLICGWIYDEALGWPEEGIPPGTAWADVPEDWKCPDCGA
                     GKSEFEMIEIGD"
     gene            complement(1706..3424)
                     /gene="leuA"
                     /locus_tag="PK144_00760"
     CDS             complement(1706..3424)
                     /gene="leuA"
                     /locus_tag="PK144_00760"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNF64283.1"
                     /translation="MTPPAGKYRPFRPLTLPDRQWPSRSIERAPRWCSSDLRDGNQAL
                     IEPMDGARKQRYFEMLVRMGFKEIEVAFPSASQTDFDFVRSLIEGGHVPDDVAIQVLT
                     QAREDLIRRTFASLKGVKRAIPHFYNATSPIFRETVFGLDRAGTIALAVRGARLFAEC
                     AAEQPETEWTFEYSPETFSFTEPGFALEICEAVLEVWQPTPQRPVILNLPATVEMATP
                     NVYADQIEWFCRHVSRRDSVIISVHPHNDRGTAVAAAELALMAGADRVEGCLFGNGER
                     TGNVDLVTLALNLYSQGIDPGLDFSQLREIVRTAEYCNQIPVHPRHPYAGDLVFTAFS
                     GSHQDAIRKGFAAQEQRVEQGDRHWEVPYLPVDPADLGASYEAVIRVNSQSGKGGIAW
                     LLEKDYGLQLPRRLQMAFSQVVQRQSDETGREQTAAAIWQAFRAEYRLDAPAPLELVE
                     HGDAPVTSGSGLDELQATLRVHGSERRVRGQGNGPIAAFVDALARQAGIGLRIADYHE
                     HATGRGADANAVAYVEAQFGEATVFGVGIDANIVTASLQAVVSAVNGAAARGLIELRR
                     PPAAAA"
     gene            complement(3534..3851)
                     /locus_tag="PK144_00765"
     CDS             complement(3534..3851)
                     /locus_tag="PK144_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64284.1"
                     /translation="MGAALVDDQGTDGGRLDKAVDNRRRCMVFCGHHADANHCHEPAT
                     ALPDPLRRIGQRLFLQVTRPGNPVRIMCCNIRNTFLRVSPPFPVCHRFVPGLLRLRSG
                     RRA"
     gene            3850..4473
                     /locus_tag="PK144_00770"
     CDS             3850..4473
                     /locus_tag="PK144_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64285.1"
                     /translation="MSASSRPPVLALAVLLLAAGSSAAAQMPLPRVDYSAVLRVSGAP
                     APVRVAHHAGVLRSEGQVGGRAVVGLLDLASGRVTLLPAGGGGPAVQLPPGTPVPGLA
                     DFDLRAFALGPARGRSQVAGHACTLHPVSERGQPVGTACVHVQDIVLAFDGTVRGHAV
                     KMETLSLSIAAQSPAAFTVPAGAGSITDWLQRLPPIPGLTAPRQPVP"
     gene            complement(4519..4594)
                     /locus_tag="PK144_00775"
     tRNA            complement(4519..4594)
                     /locus_tag="PK144_00775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4559..4561),aa:Ala,seq:ggc)
     gene            complement(4655..4730)
                     /locus_tag="PK144_00780"
     tRNA            complement(4655..4730)
                     /locus_tag="PK144_00780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4694..4696),aa:Glu,seq:ttc)
     gene            complement(4803..4878)
                     /locus_tag="PK144_00785"
     tRNA            complement(4803..4878)
                     /locus_tag="PK144_00785"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4842..4844),aa:Glu,seq:ttc)
     gene            complement(4939..5014)
                     /locus_tag="PK144_00790"
     tRNA            complement(4939..5014)
                     /locus_tag="PK144_00790"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4979..4981),aa:Ala,seq:ggc)
     gene            complement(5100..6761)
                     /locus_tag="PK144_00795"
     CDS             complement(5100..6761)
                     /locus_tag="PK144_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNF64286.1"
                     /translation="MSTPPNHFIRQIIDEDLAAGRLPAAGLRTRFPPEPNGYLHIGHA
                     KSICLNFGLARDYGGQCNLRFDDTNPCKEEVEFVEAIARDVRWLGFDWDSRQFYASDY
                     FERLYACAEALIGKGLAYVDDLTAEQIREYRGTLTEPGRDSPYRTRSAAENLDLFRRM
                     RAGEFADGTRVLRAKIDMASPNINLRDPALYRIRHEHHHQTGDAWCIYPMYDFAHALS
                     DAFEGITHSICTLEFEDHRPLYDWCVANCPVEATPHQYEFSRLTLQYAVTSKRRLTQL
                     VADGTVSGWDDPRMPTIAGLRRRGVPPAAIREFCERIGVTKSDNAVEMEMFEACVREE
                     LDRTAPRRMAVLRPLKLTLANWPAGQVEWLTAASHPKDDTFGTRELPFTGELYIDRED
                     FLESADKKWKRLVSGDSVRLRNAYVIRCDEVLKAPDGTITELRCHCFPDSLGVNPDGV
                     KPRGVIHWVSASHGVPAEVRVYERLFTTAFPDREDGDFRQFVNSDSLHVFAAVVEPAL
                     AQAGAEARFQFEREGYYCRDSREPGLVFNRTVGLRDTWQRKAGSE"
     gene            complement(6814..8250)
                     /gene="gltX"
                     /locus_tag="PK144_00800"
     CDS             complement(6814..8250)
                     /gene="gltX"
                     /locus_tag="PK144_00800"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNF64287.1"
                     /translation="MQRFPAAGPIATMVKTRFAPSPTGFLHIGGARTALYSWLHARRH
                     GGQFVLRIEDTDLERSTPEAVAAILDGMAWLGLDHDEGPYFQTQRFGRYREVIAQMLA
                     AGTAYRCYCTKEELDALRAEQMAQKIKPRYDGRYRDYDGPPRPGVEPVIRFKNPLDGE
                     VVIDDLVHGPITIRNDELDDLVIARADGTPTYNFCVVVDDMDMGITHVIRGDDHINNT
                     PRQINILRALGATVPSYAHVAMILGDDGQKLSKRHGAVSVLQYRDEGFLPGAVLNYLV
                     RLGWSHGDQEVFTREEMIRLFDIGDVNKAASAFNTSKLLWLNQQYLMSTPPEQIAWLL
                     RPFLSALGIDPDSGPDPVEVVREMACRAQTLQELAAQSAFFWQDFEAYDEKSARQHLK
                     PEAAQPLACLRERLAALPAWEPAALHETVQAVATELELKMGKVAQPLRVAVVGRAASP
                     GIDVTLALTGREAVLRRLDRALAHLAAA"
     gene            complement(8336..8755)
                     /locus_tag="PK144_00805"
     CDS             complement(8336..8755)
                     /locus_tag="PK144_00805"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNF64288.1"
                     /translation="MTATWILVADASRARLFSVSKSIGPLQEIGSFDHPGARAKTRDL
                     LADGQCSGNGSGDRECAEPRRNEALNFARELSEHLRLSRVQGLFDRLYIAAAPAFLGL
                     LRGRLDSPTAQLVIDEVSKDLTQLAPDAIRRHLPERL"
     gene            complement(8926..9648)
                     /gene="phoU"
                     /locus_tag="PK144_00810"
     CDS             complement(8926..9648)
                     /gene="phoU"
                     /locus_tag="PK144_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNF64289.1"
                     /translation="MSVSTDGHTVKRFDQQLASLRSLVLEMGGLVEDQINRAVQALQD
                     EDIAAAREVVARDHVVNGFDVRADEEVVNLLALRQPVGSDLRMVLSMAKTVTDLERIG
                     DEAQKIARMVTQIYDGNSAPNARLLRDVANMARLAANMLHGALDALARMDVDKAVEIA
                     CGDEELDSEFQSALRRLVTFMMEDPRTIGHAIDVLFMIKALERIGDHSKNIAEYVVYL
                     VKGKDVRHVTIETLAAQGRNGS"
     gene            complement(9685..10527)
                     /gene="pstB"
                     /locus_tag="PK144_00815"
     CDS             complement(9685..10527)
                     /gene="pstB"
                     /locus_tag="PK144_00815"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNF64290.1"
                     /translation="MPELHPQPEADHTAPAPVGHRTVGTVTVDNPKIAARDVNVFYGE
                     KHAIKHVDIDIGINEVIAFIGPSGCGKSTFLRCLNRMNDTIDGCRVEGAITLDGQDIY
                     DRELDVVQLRARVGMVFQKPNPFPKSIYDNVAYGPRIHGMASSRSELDDLVQWSLEKA
                     GLWNEVKDRLQQPGTGLSGGQQQRLCIARAIAVSPEVILMDEPCSALDPIATAKIEEL
                     IDELRENYSIVIVTHSMQQAARVSQRTAYFHLGDLIEVGETEQIFTNPVHKLTEGYIT
                     GRFG"
     gene            complement(10612..11871)
                     /gene="pstA"
                     /locus_tag="PK144_00820"
     CDS             complement(10612..11871)
                     /gene="pstA"
                     /locus_tag="PK144_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNF64291.1"
                     /translation="MHTALVRRRKAAERRFRAYGAGALILSLGFLVLLFTNIIGTGAS
                     AFRQAYVRLDVDFAAAELSPDGRRDPETLAAANYPGLVKAAVRAMFPEVTSRGEQRDL
                     QELVSSGAAFQLRDQVLADPSVIGTRASVWVPASALLDGLLKGSYDRSAPEAERRIND
                     RQIAWADRLAADHRLELRFNRILFTNGDSREPEQAGILGAVMGSLFTMLVTLALSFPI
                     GVAAAIYLEEFAPRNRWTDLIEVNINNLAAVPSIVYGLLGLAVFINFLGLPRSAPLVG
                     GLVLTLMTLPVIIIASRAALTSVPPSIREAALGMGASRMQTVLHHVLPLALPGMLTGA
                     IIGMARALGETAPLLMIGMVAFIVDVPGSLTAPATALPVQIYLWADSPERAFVERTSA
                     AIIVLLAFMVLMNLLAVILRRRFERRW"
     gene            complement(11937..13319)
                     /gene="pstC"
                     /locus_tag="PK144_00825"
     CDS             complement(11937..13319)
                     /gene="pstC"
                     /locus_tag="PK144_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNF64292.1"
                     /translation="MTPSLTLALLLGLTALAYHLGRRRAFTVAGGRVNRLHSLPGYYG
                     LHAALWCALPALALFLIWISLEGGLLTRLVIAGLPEALREQPPAQLALLLNDIRNVVA
                     GSVEPAAARPEVAAAAARYLDLRAGAGYGLAVLVTLLGLAGLHHAWRRVTPGFRARNH
                     VEQAGMWFLIACASVAILTTVGIVLSVLFESLLFFRKVPVTDFLFGLDWSPQTAMRAD
                     QAGASGSFGMVPLFTGTLLISAIALLVAVPLGLMSALYLSEYAPPRVRTWGKPLLEVL
                     AGIPTVVYGFFAALTVAPFIRELGTAIGLEVASESALAAGLVMGIMIIPFVSSLSDDV
                     INAVPQAMRDGSYALGATRSETIRQVVLPAALPGIVGSVLLAASRAIGETMIVVMAAG
                     LSANLTANPLDSVTTVTVQIVTLLVGDQEFDSPKTLASFALGLVLFVVTLLLNIVALH
                     VVRKYREQYE"
     gene            complement(13456..14505)
                     /locus_tag="PK144_00830"
     CDS             complement(13456..14505)
                     /locus_tag="PK144_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNF64293.1"
                     /translation="MNKPLLALAVTASLAAAAVVQAQSARDYISVVGSSTVYPFTTVV
                     AEQFGKSTKFKTPKIESTGTGGGFKLFCAGVGPEHPDVSNASRAIKTSEIESCAKNGV
                     TDIVEVKIGYDGIVFANAKGAPWVKLTRAQIYLALAKTVPDPKDPTKLVANPYQNWSQ
                     IDAGLPNAKIEVLGPPPTSGTRDAFVELVMDHGCETFPAIKALKDGDPKQFKAVCQTM
                     REDGSFIEAGENDNLIVQKLVANKSAFGIFGYSFMEENLDKVQGSIVEGHSPTFEEIA
                     AGKYPIARPLFFYVKKAHVGVIPGLKEFVNEFVSEKSFGTDGYLADKGLIPLPDAERK
                     SVTAAARELKTIGSK"
     gene            complement(14729..17419)
                     /gene="glnD"
                     /locus_tag="PK144_00835"
     CDS             complement(14729..17419)
                     /gene="glnD"
                     /locus_tag="PK144_00835"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNF64294.1"
                     /translation="MPASPDSVAAAAAGTELLDLAALGAALAAAGPPPVRAFREALRA
                     ADTRLRERFRQGVHARVLVPLRARLVDRLLALLWRHFIGEARTDLALLAVGGYGRGEL
                     HPGSDVDIMLLLAAPEDAGTGAAIEQLLTFLWDIGIEVGQSVRTVEDCVRECTSDITV
                     ATNLMEARLITGPEALYADMRTRIAPGRVWPPAAFFEAKRAEQQQRYRKFHDTEYNLE
                     PNVKEGPGGLRDLQMIGWVAKRHFGARTLHGLIAHGFLTDAEFTELSEYQDFLWQVRF
                     GLHAITGRREDRLLFDHQRTLAKLFGYADHGGQLAVERFMQDYYRTVMALSQLNEMLL
                     QFFEEAILHEDAEPVPLNRRFQVRNGYLEVTRPNVFLRYPVALLEVFLLQQQHAEIRG
                     VRAETIRLIRAHRPLIDDRFRSDLRARALFMEIMRQPKRLTRQLRRMNKYGILAAYLP
                     EFGSITGRMQHDLFHTYTVDQHTLFVIRNLRRFYLPQFENEFPHCTAVCRRLPKPELL
                     YIAGLYHDIAKGRGGDHSVLGADDAEAFCERHGLSRYDTNLVAWLVRNHLIMSLTAQK
                     KDIQDPDVIHAFAQRVGDQTHLDYLYLLTVADIRATNPSLWNHWRASLLRDLYESTRQ
                     ALRRGLVTPLDKEEHIRDVQAEARRILGRTITDDSRIDQVWAQFGDDYFLRYAPDEIA
                     WHTRAVLKKRDDGRPLVLVLARKDERRGGTEIFIYARDQKDLFARVTAALDALNLNVL
                     DARIITSLGDYTLDSFTVLEDTGEPISGRTRMREIMQRLRWQLQHPEAPLAPPSRRAP
                     RALRHFRTPTTVYFAEDAAQRHTVLTLITTDRPGLLCRIGHAFMHCGIRVHNARIATI
                     GARAEDIFLITDDAQRPLADPLSQTRLREALERQLDGDPI"
     gene            complement(17437..18204)
                     /gene="map"
                     /locus_tag="PK144_00840"
     CDS             complement(17437..18204)
                     /gene="map"
                     /locus_tag="PK144_00840"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNF64295.1"
                     /translation="MAISIKTPEEIEQMRVAGRLAAELLAMIEPHVREGVTTEELDRL
                     CHEYTVNVQQAIPAPLNYRGFPKSICTSVNHVVCHGIPGPKKLKRGDIVNVDVTVIKD
                     GWHGDTSRMFEIGEVPVIAKRVCRISKECMLIGIEMVRPGVRLGDIGHAIQRHAEANG
                     CSIVQEYCGHGIGRVFHEDPQVLHYGKPGSGPELVPGMTFTIEPMVNAGKRHVKLLPD
                     GWTVVTKDHSLSAQWEHTVAVTEHGVDVLTVRPGDPL"
     gene            18485..19231
                     /gene="rpsB"
                     /locus_tag="PK144_00845"
     CDS             18485..19231
                     /gene="rpsB"
                     /locus_tag="PK144_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNF64296.1"
                     /translation="MSTVSMRDMLEAGVHFGHQTRYWNPKMGAYIFGARSKIHIVNLE
                     KTLPLFNDAMNFVGKLAGNGGRILFVGTKRSARTAVAEAAARCGMPYVNHRWLGGMLT
                     NFKTVRQSIKRLKDLEQMSADGSFERLSKKEVILLRREMEKLERSLGGIKDMPALPDA
                     LFVIDVGHEKIAIQEAGKLGIPVVGIVDTNNSPDGVDYVIPGNDDAIRAITLYADGAA
                     AAVMTARGALPVAARADEDFVEVPAERTAG"
     gene            19344..20225
                     /gene="tsf"
                     /locus_tag="PK144_00850"
     CDS             19344..20225
                     /gene="tsf"
                     /locus_tag="PK144_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNF64297.1"
                     /translation="MSISAAMVKELRERTGSGMMECKKALTETGGDIEAAVELMRKQG
                     LAKADKKAGRTAAEGTLVVAAGDKAVALVEVNCETDFVAKGDEFQGFAAAVAASALTA
                     SPADLDALLAAPLADGKSVDEIRREKVAKIGENIAVRRFVRYESAGGVLASYLHGTRI
                     GVLVELAGGDEELAKDVAMHIAASRPLCVSPEQVPAEVVEKEREIALANAVNEGKSPE
                     IAAKMVEGRVRKFLNENTLLGQVFIKDPEGKQTVAALLKGRGASVVAFTRYELGEGIE
                     KKQENFADEVMAQVRGS"
     gene            20319..21059
                     /gene="pyrH"
                     /locus_tag="PK144_00855"
     CDS             20319..21059
                     /gene="pyrH"
                     /locus_tag="PK144_00855"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNF64298.1"
                     /translation="MDFPMPDARPAYKRILLKLSGEALMGEGDFGIDPGVIHRIANEV
                     GQVNRMGVEVGMVIGGGNLFRGAGLAEAGMDRVTGDHMGMLATVMNSLAMQDALERIG
                     VFARVMSAVRINQVCEDYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDSAASLRAIEIN
                     AEVMLKATKVDGIYTADPKKDPTATRYDTITYDEAITRKLQVMDTTAIVMCREHSMPL
                     KVFDLNRPDEIVRVVCGVPGIATTVVRG"
     gene            21069..21626
                     /gene="frr"
                     /locus_tag="PK144_00860"
     CDS             21069..21626
                     /gene="frr"
                     /locus_tag="PK144_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNF64299.1"
                     /translation="MINDIKKDAASRMEKSVDALRHELAKLRTGRAHVSLLDHVHVEY
                     YGNSVPLSQAASINVSDARTLVVQPWEKTMVGPIEKAIMTSDLGLNPVTVGTVIRVPL
                     PALTEERRREMGKVVRHEGESAKVAVRNIRRDAIADLKMLQKEKEISEDDERRGTDDV
                     QKITDRFVEEIDKLVAAKEAELLEI"
     gene            21665..22414
                     /gene="uppS"
                     /locus_tag="PK144_00865"
     CDS             21665..22414
                     /gene="uppS"
                     /locus_tag="PK144_00865"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNF64300.1"
                     /translation="MSSSDSTPGTLPRHVAIIMDGNGRWAKNRKLPRPFGHREGVKAL
                     RRVVQHGIDRGLDAMTLFAFSSENWRRPALEVKLLMELFSVVLRSEMKRLMDNNARLR
                     FVGDRTPFSPRLREAMIQAEETTGRNTGMTVVIAANYGGRWDIANAARELAVEVAAGR
                     LQPEDIDAGLIQRHISLADLPEPDLFIRTGGEERISNFLLWQMAYTEFYFTDCLWPDF
                     DEAALDAAFDSFGRRQRRFGRTGDQVEQRGA"
     gene            22407..23231
                     /locus_tag="PK144_00870"
     CDS             22407..23231
                     /locus_tag="PK144_00870"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNF64301.1"
                     /translation="MLRQRILTALVLAAVAILGVLWLPSGVLMLLVAGIVALGAREWL
                     QLIGLPGHGARIAFHGAWLVLLLLGWLGGETGFGLLLVLLAALFWLLPVPLWLRRYAA
                     EPALAQPREWWVISGLVVLAGGGVAFTVLHDAPGYGPGWVLLLMFLVAGADIGAFFAG
                     RRFGRVKLAPQISPGKTREGAWGALAATALVALAGAAALGVPAARWPAFLLVCALTVI
                     WSILGDLFESMLKRQHGAKDSGTLLPGHGGVLDRIDSLTAAAPVFLLGLTVFGPWR"
     gene            23228..24436
                     /gene="ispC"
                     /locus_tag="PK144_00875"
     CDS             23228..24436
                     /gene="ispC"
                     /locus_tag="PK144_00875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNF64302.1"
                     /translation="MSARPLAVTVLGATGSIGLSTLDVLARHPDRYRVHALSAHRNVD
                     ALAGLCERHRPEYAVLPESSAASRLQARLHAAGCRTEVLAGAEALAAVAAAPETDAVM
                     AAIVGAAGLLPALAAARAGKRVLLANKEALVMSGALFMEAVRAHGAQLLPIDSEHNAI
                     FQCLPPAAQAGGRPEGVTRLLLTASGGPFRDTPVDEIPHKTPAEACAHPNWSMGRKIS
                     VDSATMMNKGLELIEAFWLFGLPRERIEVVIHPQSVIHSMVEYADGSVLAQLGNPDMR
                     TPIAHALAWPERIASGVAPLDLFRIGRLDFRAPDLLRYPCLGLAQAALAAGGTAPAIL
                     NAANEVAVAAFLDGRLGFGGIAQLNAAVLDAVPAAAADTLDAVLAADAAARRAAEARI
                     DGDKLAGGVR"
     gene            24433..25791
                     /gene="rseP"
                     /locus_tag="PK144_00880"
     CDS             24433..25791
                     /gene="rseP"
                     /locus_tag="PK144_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019022793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNF64303.1"
                     /translation="MNGPLLSFLALVVTLGVLITVHEFGHFWVARRCGVRVLRFAIGF
                     GRPLWRRTARDGVEYVIGAVPLGGYVRMLDEREGEVPPEQLDQAFNRQTVGRRAAIVA
                     AGPAFNFAFAILAYLILYLSGVSQVRPVLAEAAAGTPAAIAGIRDGDEVLAVGDVTVQ
                     GLDELLMRVAEQMEPGEALKLRVRDADGRVLERRLPLPEDLDTDGNLLARLGLQPWRP
                     SLPAVIGSIKPDSPAAAAGLQDGDRIVSVDDQRVADWQDFATRVRAKPEADLRLQIER
                     DGSPLSVRLRPVRIETAQGAVGQIGAFAAVPQSLADSLRVVVRYGPIDAAVAAVERTW
                     EMSAFTLRMLGRMLTGRASLDNLSGPLTIAQMAGQSASIGLSAFLSFLALVSISLGVL
                     NLLPVPVLDGGHLAWYAIETIKGSPVSEAVQSAGQRVGLAVLLVMMGLAFYNDLARLF
                     GP"
     gene            25840..28140
                     /gene="bamA"
                     /locus_tag="PK144_00885"
     CDS             25840..28140
                     /gene="bamA"
                     /locus_tag="PK144_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNF64304.1"
                     /translation="MTPILRNFLLSASLAAPACIVQAAEFIVRDIRVEGLQRISAGTV
                     FSYLPVQTGQRVRQEDFPDLIRTLFRTGFFTDVSLQRDGDVLVVNVVERPAIAEVKVT
                     GNKDISTEDLEKSLKTVGLAEGRTFDRALLDKVEQELNRQYYARGKYAVKVDSQVRPL
                     ERNRVAITIDISEGIAARIKQINIVGAKAFDEDDLLDLFQSSTSNWTSLLSKDDQYSK
                     QKLGGDLETLRSYYLDRGYLRFNIDSTQVTITPDKKDIYITINIAEGDRYTVKDVRLA
                     GESPVPLDQLKPLVKLQPGQTFSRATLNESTKAITDRLGDDGYAFANVSAVPQIDDAG
                     KTVSLALNVDAGRRVYVRRINFKGNIKTQDEVLRREMRQTEGSWFATRDVNRSKERLE
                     RLAFVQEVTVETPAVPGTPDQVDLNYSVTERPAGSLSFGIGYGQDAGVLLSASVSQQN
                     FLGTGNALSFTFNNSQINTIYSFSYTNPYFTPDGVSAGIRASYRETDASEANLASYLT
                     NEFETMATFGFPLNETDTVNLAIGISGIKLKTNGDNTPQGIYDYIDEHGERHVGPRFE
                     LSWARDTRNKAVFADRGSYNRLSIESMLGGDDSWYKLGYRHSTYLPLTDSLTLLGHFD
                     FGYGEGFGNTDTLPFYENFYAGGLRTVRGYKTNTLGPRWDDPNPDLRDPKGGALRTAG
                     GVEVLFPAPFFKDNKNLRFSTFFDIGNVYAEVNDFDAGELRYSAGLSMQWITPLGPLV
                     MSVAQPLNKKDGDKTEPFQFTFGVPF"
     gene            28172..28687
                     /locus_tag="PK144_00890"
     CDS             28172..28687
                     /locus_tag="PK144_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF64305.1"
                     /translation="MKALLRVLLGALALTFALPAVAAELKLGFVNAARVLKSAPQAEA
                     ALNRLEKEFSPRDKALVEAQKALRKLEDRLSKDGATLSDAERRDLERDIISQRRDVKR
                     AQDEFREDFNIRRNEEMAKLQKLIQDAITALAQEERYDLVLNDGAVIFASEAVDITDK
                     VLKRLSAVHGN"
     gene            28708..29736
                     /gene="lpxD"
                     /locus_tag="PK144_00895"
     CDS             28708..29736
                     /gene="lpxD"
                     /locus_tag="PK144_00895"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF64306.1"
                     /translation="MHTLAELAQVAGAQLRGDAATPIDGVGTLAAARPGQIGFLANPK
                     YRQLLSGTQASAVILGAEDAGACPVACLIADNPHLAYARVAALFDPAAGRQQGVHARA
                     CVDAAAEVADDAWIGPMAVIEAGAVIGPRVQVGPGCVVGARTSIGADSRLVANVTVLH
                     DCVLGERVLVHPGAVIGADGFGLANDRGCWVKVPQLGRVVIGNDVEIGVNTAVDRGAL
                     EDTVLADGVKLDNLIQIAHNVRVGAHTAMAGCVGIAGSTTVGAHCTLAGGVGLAGHLT
                     LGDHVHVTGMSLVTKSLTEPGVYASGLAVEPARLWNKVSARLRRLDELFRRLAALEKR
                     VGDDAGHP"
     gene            29758..30198
                     /gene="fabZ"
                     /locus_tag="PK144_00900"
     CDS             29758..30198
                     /gene="fabZ"
                     /locus_tag="PK144_00900"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011717683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNF64307.1"
                     /translation="MDITQVLRYLPHRYPFLLIDRVLELEPGERLLGLKNVSFNEPFF
                     QGHFPQQPVMPGVLILEALAQATGILAFKTSGQLPEDDSTYYFVGIDNARFKQPVCPG
                     DRLLLDVRLLKTRRGIWVFSAEARVDDKVVCSAELMSTKREHGQ"
     gene            30195..30965
                     /gene="lpxA"
                     /locus_tag="PK144_00905"
     CDS             30195..30965
                     /gene="lpxA"
                     /locus_tag="PK144_00905"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNF64308.1"
                     /translation="MIDPRAIVDPGARIAEGVQIGPFSIIGAGVEIETGTVIGPHVVI
                     KGPTRIGRDNRIYQFASIGDDPQDKKYAGEPTRLEIGDGNTIREYVTINRGTVQDAGV
                     TRVGHDNWIMAYVHIAHDCVVGDHTIFANNASLAGHVHVGDWAILGGYTLVHQFCRIG
                     AHSFCGFATGVQRDVPPYVTVAGYRAEPYGINSEGLRRRGFEAETIAVIKRAYKLLYM
                     SDLKLAEAVERMRELVATHPPVALLADFVADSPRGIVR"
     gene            30971..>31408
                     /locus_tag="PK144_00910"
     CDS             30971..>31408
                     /locus_tag="PK144_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF64309.1"
                     /translation="MRIAVVAGELSGDALGGALLPALQQRFPQARFEGIGGPRMQAAG
                     LESLFPLEKLSVMGLVEVLKHLPELLSIRRALVRRWLAEPPALYLGIDAPDFTLPLAK
                     RLRAHGIRTAHYVSPSVWAWRQGRVRGIAQSVDLMLTLFPFEAT"
BASE COUNT         4816 a        10506 c        11173 g         4913 t
ORIGIN      
        1 ctgttcacgg ccagcgcatc ggcgccggcc tcgaccaggc cggcgccgtt gccggcgtcg
       61 atgccgccga tcgcgacgat cgggatgcgg cgcgcgagcg catggcgcgc ctcgccgagc
      121 agcgacagcg gcgcgcgcac ggcgtcgggc ttgatcgcgg acggataaaa ggcgccgaag
      181 gccacgtagt cggcgccggc tgcgcaggct gcgcgggcac gctcgaggct gtcgtagcag
      241 gacacgccga tcagcgcccg cgctccgagc cggcgccgtg ccgcggcgaa ggcggcgtcg
      301 tcacggccga ggtgcacgcc gtcggcgccg gtctcggcgg cgagggccac gtcgtcgttg
      361 atgatcagcg tcacggcgcg ggcgcggcag agtccggcga gtgccgcggc gagctgccgg
      421 cgcagcgcgg catcggcgcg cttgtcgcgg tactggatcg tgcgcgcgcc gccgtcgatg
      481 gccgccgaga cccgttcgag gagctgcccg tccgggatca ggccgggatc ggtgatcgcg
      541 tacaggccgt gcgggcgcgg ccgttcagtc atcggcgccg tcctcgtcgg cccagaacag
      601 ccggttcggg atgcgctggc cggcgccgat gcggtagccg ccgcgcagcg cctcccaggt
      661 gtattcctgc gcctgcgcga ccgcggcgtg cagctcgttg ccctgcgcga gcaggccggc
      721 gagcgcggcg gccagcgtgc agccggagcc gtggaagtcg tgcggcaggc gctcccaggt
      781 ccagctgtcg agcaggcggc cgtcgccgta gaggcggttg acgacggcat cggtggtctc
      841 gtgcgtgccg gtgatcagca cgtgcgcaca gccggtgtcg agcagcgcct gcgcgcaggc
      901 atcgagggtg tcggcggccg gggcgagccg gcgggcctcg acgctgttcg gggtcagcac
      961 cgtcgtgcgc gccagcagat gccggcggat cgcatcggcg agcgcatcgc cgtcgaaatc
     1021 gcgcccgccg ccggcggcga gcaccgggtc gaggatcagc gggatgtgcg gatagtcttc
     1081 gagcagctcg gcgatgatct cggcgttggc cggcgagccg agcaggccga tcttgatcgc
     1141 tgcgaccggc gcgtcattca gcaccgtgcg cgcctgcgcg tagacgaggt cgggatcgat
     1201 cggcaccacg gcgagcacgt cgcaggtgtc ctggacggtc agcgccgtca ccaccggcgc
     1261 tgcgtggcag cccatgctgg cgatggcctc gatgtcggcc tgcaggccgg cgccaccgct
     1321 cgggtcgttg ccggccagcg tcatgaccac cggtaccgtg tagctctggc tcatcgcggg
     1381 cgtcctgaag gcttgaagca ggggagctgg ccatgtcgaa gtgcgcggcg tccccgcacc
     1441 cggacgtttt gcggcacgat aaccgatcct taccgccctc gcgaggtagt gcccgtgaag
     1501 cagtacatgt gcctgatctg cggctggatt tacgacgagg cgctgggctg gcccgaggaa
     1561 ggcatcccgc cgggcacggc ctgggccgac gtgcccgagg actggaagtg tccggactgc
     1621 ggcgcgggca agtccgaatt cgagatgatc gagatcggcg actgaaggca ggccggggcg
     1681 gtgtgcgcac cgccccggcg caggttcagg ccgcggccgc cggcggccgg cgcagctcga
     1741 tcagcccgcg tgccgccgcg ccgttgacgg ccgagaccac cgcctgcagc gaggcggtga
     1801 cgatgttggc gtcgatgccg acgccgaaca cggtggcctc cccgaactgc gcctcgacgt
     1861 aggcgacggc gttggcatcg gcgccgcgcc cggtggcgtg ctcgtggtag tcggcgatgc
     1921 gcagcccgat gccggcctga cgcgcgagcg catcgacgaa ggccgcgatc ggaccgttgc
     1981 cctgaccgcg cacgcgccgc tcgctgccat gcacgcgcag cgtcgcctgc agctcatcga
     2041 ggcccgagcc gctggtgacc ggcgcatcgc cgtgctcgac cagttcgagc ggggccggcg
     2101 catcgagccg gtattcggcg cggaaggcct gccagatcgc cgcggcggtc tgctcgcggc
     2161 cggtctcgtc gctctggcgc tgcacgacct ggctgaaggc catctgcagc cggcgcggca
     2221 gctgcaggcc gtagtccttt tcgagcagcc aggcgatgcc gcccttgccg ctttgcgagt
     2281 tgacgcggat caccgcctcg tagctcgcgc cgagatcggc cggatcgacc ggcaggtacg
     2341 gcacctccca gtgccggtcg ccttgctcaa cgcgctgctc ctgcgccgcg aagcccttgc
     2401 ggatcgcatc ctgatgcgaa cccgagaagg ccgtgaacac gagatcgccg gcgtacggat
     2461 ggcgcgggtg taccggaatc tggttgcagt actcggccgt gcgcacgatc tcgcgcagct
     2521 gcgagaagtc cagacccgga tcgatgccct ggctgtagag gttcagcgcc agcgtcacga
     2581 gatcgacgtt gccggtgcgc tcgccgttgc cgaacaggca gccctcgacg cgatcggctc
     2641 cggccatcag cgccagctcg gccgcggcga ctgcggtgcc gcggtcgttg tgcggatgca
     2701 cgctgatgat gacgctgtcg cggcgcgaga cgtgccggca gaaccattcg atctggtcgg
     2761 cgtagacgtt cggcgtcgcc atctcgacgg tcgccggcag gttcaggatc accggccgct
     2821 gcggcgtcgg ctgccagact tcgagcaccg cctcgcagat ctcgagcgcg aagccgggct
     2881 cggtgaacga gaaggtttcc ggggaatact cgaaggtcca ctcggtttcg ggctgctcgg
     2941 ccgcgcattc ggcgaacagc cgcgcaccgc gcaccgcgag cgcgatcgtg ccggcgcgat
     3001 cgagtccgaa cacggtctcg cggaagatcg gcgaggtggc gttgtagaag tgcgggatcg
     3061 cacgcttcac gcccttcagg ctcgcgaagg tgcggcggat cagatcctcg cgcgcctgcg
     3121 tcagcacctg gatcgcgacg tcatcgggca cgtggccgcc ctcgatcagg ctgcgcacga
     3181 agtcgaaatc ggtctgcgag gccgacggga aggcgacctc gatctccttg aagcccatgc
     3241 gcaccagcat ctcgaagtag cgctgcttgc gcgcgccgtc catcggctcg atcagcgcct
     3301 ggttgccgtc gcgcaggtcg gacgagcacc agcgcggcgc gcgctcgatg ctgcgcgacg
     3361 gccactggcg atcgggcagc gtcagcggcc ggaacggccg gtacttgccg gcgggcgggg
     3421 tcatcgcgga aacgttcggg ttcgtgtggt tcatcggatt cgtctcgggc ggcgtgttcg
     3481 gttcggtatg catgcgggac acggcaggtg cacggccggc cgccgggcag ccgtcaggcc
     3541 cggcgaccgc tgcgcagtcg cagcaggccc ggaacgaacc gatggcaaac agggaaaggg
     3601 ggcgaaacgc gaaggaaggt gttacggatg ttgcagcaca tgatgcgtac cggattgcct
     3661 gggcgggtaa cctgcaggaa cagccgctgc ccgatcctcc tcagaggatc gggcagggca
     3721 gtcgcaggtt cgtggcaatg gttcgcatct gcatgatgcc cgcagaaaac catgcaccgg
     3781 cggcgattgt caacggcttt gtccagccgt ccgccatccg ttccttgatc gtccaccagg
     3841 gccgcaccca tgtccgcaag ctcccgtccg cccgtgctgg cgctcgccgt gttgctgctt
     3901 gccgccggca gcagcgccgc agcgcagatg ccgctgccgc gcgtcgatta cagcgccgtg
     3961 ctgcgcgtct ccggcgcgcc cgcgccggtg cgcgtcgccc atcacgccgg cgtgctgcgc
     4021 agcgaaggcc aggtcggcgg gcgcgccgtg gtcggcctgc tcgatctcgc cagcgggcgc
     4081 gtgacgctgc tgccggccgg cggcggcggt ccggccgtgc agctgccgcc gggcacaccg
     4141 gtgcccggcc tcgccgattt cgacctgcgc gcctttgcgc tcggtccggc gcgcggccgc
     4201 agccaggtcg ccggccatgc ctgcacgctg catccggtca gcgagcgcgg ccagccggtc
     4261 ggcaccgcct gcgtgcatgt gcaggacatc gtgctcgcct tcgacggcac ggtgcgcggc
     4321 catgccgtga agatggagac gctgtcgctg tcgatcgccg cgcagtcgcc ggcggccttc
     4381 acggtcccgg ccggggccgg ctcgatcacg gactggctgc agcgcctgcc gccgattccg
     4441 ggcctgaccg cgccgcgcca gccggtgccg tgaacgacgg ccaaagaaaa agccgcccct
     4501 cggggcggct ttggagtttg gtggagctaa tcgggatcga accgatgacc tcctgcatgc
     4561 catgcaggcg ctctcccagc tgagctatag ccccggattc cgtgaatacc gggtcgcctc
     4621 ctggcgtctt cgggctgtcc gtgccggtgt ccgatggcgt ccccaagggg attcgaaccc
     4681 ctgtcgccgc cgtgaaaggg cggtgtcctg ggcctctaga cgatggggac acaggaaact
     4741 gctgcagacg ctcctgtctg cggcccgcct gccggcagcg gctggcagcg gggtgatcaa
     4801 gatggcgtcc ccaaggggat tcgaacccct gtcgccgccg tgaaagggcg atgtcctggg
     4861 cctctagacg atggggacgc aggaaacagc ttcaaaaaac aggaaaccgt cggtaagcga
     4921 cggtttccct tggcattctg gtggagctaa tcgggatcga accgatgacc tcctgcatgc
     4981 catgcaggcg ctctcccagc tgagctatag ccccacggtg ctgaggagcc gccattctat
     5041 gaatccgcgg accgaagtca accgctccgg tgaaactccg gcaccgaaag ggccggtcct
     5101 cattcgctgc cggccttgcg ctgccaggtg tcgcgcaggc cgacggtgcg gttgaacacc
     5161 agcccgggct cgcggctgtc gcggcagtaa taaccttcgc gctcgaactg gaagcgcgcc
     5221 tcggccccgg cctgcgcgag cgccggttcg acgacggccg cgaacacgtg cagcgagtcg
     5281 ctgttgacga actggcggaa gtcgccgtcc tcgcggtccg ggaaggcggt cgtgaacagg
     5341 cgctcgtaga cgcgcacctc ggccggcacg ccgtggctcg ccgacaccca gtggatgacg
     5401 ccgcgcggct tgacgccgtc cgggttgacg ccgagcgagt ccgggaagca gtggcagcgc
     5461 agttcggtga tcgtgccgtc cggggccttg agcacctcgt cgcagcggat cacgtaggcg
     5521 ttgcgcaggc gcacgctgtc gccgctgacc aggcgcttcc atttcttgtc ggcggactcc
     5581 aggaagtcct cgcggtcgat gtacagctcg ccggtgaacg gcagctcgcg cgtgccgaag
     5641 gtatcgtcct tcgggtggct cgcggccgtg agccattcga cctgtccggc cggccagttg
     5701 gcgagcgtca gcttcagcgg ccgcagcacg gccatgcgcc gcggcgcggt gcgatcgagt
     5761 tcctcgcgca cgcaggcctc gaacatctcc atctcgacgg cgttgtcgct cttggtgacg
     5821 ccgatgcgct cgcagaactc gcggatcgcc gccggcggca cgccgcggcg gcgcaggccg
     5881 gcgatggtcg gcatgcgcgg atcatcccag ccgctgaccg tgccgtcggc gacgagctgc
     5941 gtcagccgcc gcttgctggt caccgcgtac tgcagcgtca ggcgcgagaa ctcgtactgg
     6001 tgcggcgtcg cctcgaccgg gcagttggcg acgcaccagt cgtacagcgg ccggtggtcc
     6061 tcgaactcca gcgtgcagat cgagtgcgtg atgccctcga aggcgtccga cagcgcgtgc
     6121 gcgaagtcgt acatcgggta gatgcaccag gcatcgccgg tctggtggtg gtgctcgtgg
     6181 cggatgcggt acagcgccgg gtcgcgcagg ttgatgttcg gcgaggccat gtcgatcttc
     6241 gcgcgcagca cgcgcgtgcc gtcggcgaac tcgccggcgc gcatgcgccg gaacaggtcg
     6301 aggttctccg cggcgctgcg cgtgcggtac gggctgtcgc ggccgggttc ggtcagcgtg
     6361 ccgcggtatt cgcggatctg ctcggccgtg aggtcatcga cgtaggcgag ccccttgccg
     6421 atcagcgcct cggcgcaggc gtacagccgc tcgaagtagt ccgaggcgta gaactggcgg
     6481 ctgtcccagt cgaagccgag ccagcgcacg tcgcgcgcga tcgcctcgac gaactcgacc
     6541 tcctccttgc acggattggt gtcgtcgaag cgcaggttgc actggccgcc gtagtcgcgc
     6601 gccagcccga agttcaggca gatcgacttg gcgtggccga tgtgcaggta gccgttcggc
     6661 tcgggcggaa agcgcgtgcg caggccggcg gccggcagcc ggccggcggc gaggtcctcg
     6721 tcgatgatct gacggatgaa atggttcggc ggggtgctca tggcggcgtc cagcgactga
     6781 tcagacaggt tccgcggcgg ggcggcggaa ggttcaggcg gcggcaagat gcgcgagtgc
     6841 gcggtcgagg cggcgcagca cggcctcgcg gccggtcagc gccagcgtca cgtcgatgcc
     6901 gggcgaggcg gcgcgtccga cgacggcgac gcgcagcggc tgcgcgacct tgcccatctt
     6961 gagttccagt tcggtcgcga ccgcctgcac ggtctcgtgc agcgcggccg gctcccaggc
     7021 cggcagcgcg gccaggcgct cgcgcaggca ggcgagcggc tgcgcagctt cgggcttcag
     7081 gtgctgtctt gcggatttct cgtcgtaagc ctcgaaatcc tgccagaaga aggcgctctg
     7141 cgcggcgagt tcctgcagcg tctgcgcgcg gcaggccatc tcgcgcacga cctcgaccgg
     7201 gtcgggaccg ctgtccgggt cgatgccgag cgccgagagg aagggccgca gcagccaggc
     7261 gatctgctcg ggcggcgtgc tcatcaggta ctgctggttc agccacagca gcttcgaggt
     7321 gttgaacgcc gaggcggcct tgttgacatc gccgatgtcg aacaggcgga tcatctcctc
     7381 gcgcgtgaag acttcctgat cgccgtgcga ccagccgagg cgcacgaggt agttgagcac
     7441 tgcaccgggc aggaagccct cgtcgcggta ctgcagcacg ctgaccgcgc cgtggcgctt
     7501 cgagagcttc tggccgtcgt cgccgaggat catcgccacg tgcgcgtagc tcggcaccgt
     7561 cgcgccgagt gcgcgcagga tgttgatctg gcgcggcgtg ttgttgatgt ggtcgtcgcc
     7621 gcggatcacg tgcgtgatgc ccatgtccat gtcgtcgacg acgacgcaga agttgtaggt
     7681 cggcgtgccg tcggcgcggg cgatgaccag gtcgtcgagt tcgtcgttgc ggatcgtgat
     7741 cggcccgtgc acgagatcgt cgatgacgac ctcgccatcg agcgggtttt tgaagcggat
     7801 caccggctcg acgccggggc gcggcgggcc gtcgtagtcg cggtagcggc cgtcgtagcg
     7861 cggcttgatc ttctgcgcca tctgctcggc gcgcagcgcg tcgagttctt ccttcgtgca
     7921 gtagcagcgg taggcggtgc cggccgcgag catctgcgcg atgacctcgc ggtagcgccc
     7981 gaagcgctgc gtctggaagt acgggccttc gtcgtgatcg aggccgagcc aggccatgcc
     8041 gtcgaggatc gcggcgacgg cctcgggcgt cgagcgttcg agatcggtgt cctcgatgcg
     8101 cagcacgaac tggccgccgt ggcggcgggc atgcagccac gagtacagcg cggtacgcgc
     8161 accgccgatg tgcaggaagc cggtggggct gggggcgaag cgggtcttga ccatcgtcgc
     8221 gatcggtccg gcagcgggaa agcgctgcat tctagcgatc cgggcggccg gcgggggggc
     8281 cggaaacgct gcgggggaga ccgtccggtc tcccccgcag cgcaggcagg aaagctcaga
     8341 gccgttcggg caggtgccgg cggatcgcgt cgggcgcgag ctgggtgagg tccttgctga
     8401 cctcgtcgat caccagctgc gcggtcgggc tgtcgagccg gccgcgcagc aggccgagga
     8461 aggccggggc cgcggcgatg tagagccggt cgaacagacc ctgcacgcga ctgaggcgca
     8521 ggtgctcgga cagctcgcgc gcgaagttca gcgcctcgtt gcgccgcggc tcggcacact
     8581 cgcgatcccc gctgccgttg ccggaacact ggccgtcggc gagcaggtca cgggtcttgg
     8641 cgcgcgcgcc ggggtggtcg aagctgccga tttcctgcag cgggccgatg gacttgctga
     8701 cgctgaacag ccgggcgcgg cttgcgtccg ccaccagtat ccacgttgcg gtcatgctca
     8761 cctccacaaa cccggaccgg agggcccggg atgccgctgg ccttcgcacg cgggggaagt
     8821 gaggcttgtc cggccatccg ccgcgcgcgg gcacgtcgga tcaacgtaga cggacccgcc
     8881 ggtgcctgcc agcggtgcgc cggagccgcc ctgggcggcg tgcgttcagc tgccgttgcg
     8941 gccctgggcg gcgagcgtct cgatcgtgac gtggcgcacg tccttgccct tgaccagata
     9001 cacgacgtat tcggcgatgt tcttggagtg atcgccgatg cgctccagcg ccttgatcat
     9061 gaacagcacg tcgatcgcat gcccgatcgt gcgcgggtcc tccatcatga aggtcaccag
     9121 ccggcgcagc gccgactgga actcgctgtc gagctcctcg tcgccgcagg cgatctcgac
     9181 cgccttgtcg acatccatgc gcgccagcgc atcgagcgca ccgtgcagca tgttggcggc
     9241 gaggcgcgcc atgttggcga catcgcgcag cagccgggca ttcggcgcgc tgttgccgtc
     9301 gtagatctgc gtgaccatgc gcgcgatctt ctgcgcctcg tcgccgatgc gttcgaggtc
     9361 ggtgaccgtc ttggccatcg acagcaccat gcgcaggtcc gagccgaccg gctggcgcag
     9421 cgccagcagg ttgacgacct cctcgtcggc gcgcacgtcg aagccgttga cgacgtgatc
     9481 gcgcgcgacc acctcgcgcg ccgcggcgat gtcctcgtcc tgcagcgcct gcacggcacg
     9541 gttgatctgg tcctcgacga ggccgcccat ttcgagcacg agactgcgca ggctcgcgag
     9601 ctgctggtcg aagcgcttga cggtgtggcc gtcggtactc acggacatgg cggatactcc
     9661 ctgggggcgg gcgggcaggg cggctcagcc gaagcggccg gtgatgtagc cttcggtcag
     9721 cttgtgcacg gggttcgtga agatctgctc ggtctcgccg acctcgatca ggtcaccgag
     9781 gtggaagtac gcggtgcgct gcgacacgcg tgcggcctgc tgcatcgagt gcgtgacgat
     9841 gacgatcgag tagttctcgc gcagctcgtc gatcagttcc tcgatcttcg cggtcgcgat
     9901 cgggtcgagc gccgagcagg gctcgtccat caggatcacc tcggggctga cggcgatcgc
     9961 gcgcgcgatg cagaggcgct gctgctggcc gccggaaagt ccggtgccgg gctgctgcag
    10021 gcggtccttg acctcgttcc agaggccggc cttctccagg ctccactgca cgaggtcatc
    10081 gagctccgaa cgcgagctgg ccatgccgtg gatgcgcggg ccgtaggcga cgttgtcgta
    10141 gatcgacttc ggaaacgggt tcggcttctg gaacaccatg ccgacgcgcg cgcgcagctg
    10201 cacgacatcg agctcgcggt cgtagatgtc ctgaccgtcg agcgtgatcg caccctcgac
    10261 gcggcagccg tcgatggtgt cgttcatgcg gttgaggcag cgcaggaacg tcgacttgcc
    10321 gcagcccgag gggccgatga aggcgatgac ttcattgatg ccgatgtcga tgtcgacgtg
    10381 cttgatcgcg tgcttctcgc cgtagaacac gttcacgtca cgcgcggcga tcttcgggtt
    10441 gtcgacggtc accgtaccga cggtgcggtg gccgacgggg gccggggcgg tgtggtccgc
    10501 ctcgggctgc gggtgaagct ccggcatcgg ggtttcctcg attcggttca gcggttccgg
    10561 tcgggtcatc gtagtgtctt ccggcatgca gatgcggtgg gtgtgctgcg cttaccagcg
    10621 gcgctcgaag cgccggcgca ggatcacggc gagcaggttc atcagcacca tgaatgccag
    10681 cagcacgatg atcgcggccg aggtgcgctc gacgaaggcc cgctcggggc tgtcggccca
    10741 gaggtagatc tgcacgggca gggccgtggc cggagcggtc aggctgcccg gcacgtcgac
    10801 gatgaacgcg accatgccga tcatcagcag cggcgcggtc tcgccgagtg cgcgcgccat
    10861 gccgatgatc gcgccggtca gcatgccggg cagcgcgagc ggcagcacgt gatgcagcac
    10921 ggtctgcatg cgcgaggcac ccatgccgag cgcggcctcg cggatcgacg gcggcaccga
    10981 ggtcagcgct gcgcgcgagg cgatgatgat caccggcagc gtcatcagcg tcagcaccag
    11041 tccgccgacc agcggcgccg agcgcggcag gccgaggaag ttgatgaaca ccgccaggcc
    11101 gagcaggccg tagacgatcg acggcaccgc ggcgaggttg ttgatgttga cctcgatcag
    11161 gtcggtccag cggttgcgcg gcgcgaattc ctccagatag atcgccgcgg cgacgccgat
    11221 cgggaacgac agcgccagcg tcaccagcat cgtgaacagc gagcccatga ccgcgccgag
    11281 gatgccggcc tgctcgggct cgcgcgagtc gccgttcgtg aacaggatgc ggttgaagcg
    11341 cagttcgagc cggtggtcgg cggccagccg gtcggcccag gcgatctggc ggtcgttgat
    11401 ccggcgctcg gcctcggggg cgctgcggtc gtagctgccc ttgagcaggc catcgagcag
    11461 cgcgctcgcc ggcacccaga cgctggcgcg cgtgccgatc accgacggat cggccagcac
    11521 ctggtcgcgc agctggaagg ccgcgccgga gctgaccagc tcctgcaggt cgcgttgctc
    11581 gccccgggac gtgacctccg ggaacattgc gcgcacggcg gccttcacga ggccgggata
    11641 gttggcggcg gcgagcgtct cggggtcgcg ccggccgtcc gggctcagct cggcggcagc
    11701 gaaatcgaca tcgaggcgca cgtaggcctg gcggaacgcg ctggcaccgg tgccgatgat
    11761 gttcgtgaac agcagcacca gaaagccgag gctcaggatc agcgcgccgg cgccgtaggc
    11821 gcggaagcgc cgctcggcgg ccttgcgccg gcgcacgagg gcagtgtgca tgctgccgcc
    11881 gagccggcgc tcgggcgcga tggcctggat gccgcgcggg gaagtggaag cctggttcat
    11941 tcgtattgct cccggtactt gcgcacgacg tgcagcgcga cgatgttcag cagcagcgtc
    12001 acgacgaaca gcacgaggcc gagcgcgaac gaggccagcg tcttcgggct gtcgaactcc
    12061 tggtcgccga ccagcagcgt cacgatctgc accgtgaccg tggtcaccga atcgagcggg
    12121 ttcgcggtca ggttcgccga caggccggcg gccatcacga cgatcatcgt ctcgccgatc
    12181 gcccgcgagg cggcgagcag caccgagccg acgatgccgg gcagcgccgc cggcagcacg
    12241 acctggcgga tcgtttcgga gcgcgtcgcc ccgagcgcgt aggagccgtc gcgcatcgcc
    12301 tgcggcacgg cgttgatcac gtcatcggac agcgaggaca cgaacgggat gatcatgatg
    12361 cccatgacca gaccagcggc cagcgcgctc tccgaggcca cttcgaggcc gatcgcggtg
    12421 ccgagctcgc ggatgaacgg cgcgaccgtc agcgccgcga agaagccgta gaccaccgtc
    12481 gggatgccgg cgagcacctc cagcagcggc ttgccccagg tgcgcacgcg cggcggtgcg
    12541 tattcggaca ggtacagcgc cgacatcagt ccgagcggca cggccaccag cagggcgatc
    12601 gccgagatca gcagcgtgcc ggtgaacagc ggcaccatgc cgaagctgcc cgaggcgccg
    12661 gcctgatcgg cgcgcatcgc cgtctgcggg ctccagtcca ggccgaacag gaagtccgtg
    12721 accgggacct tgcgaaagaa cagcagcgat tcgaacagca ccgacagcac gatgccgacc
    12781 gtggtcagga tcgcgacgct cgcgcaggcg atcaggaacc acatgccggc ctgctcgaca
    12841 tggttgcggg cgcggaaacc cggggtgacg cggcgccagg cgtggtggag gccggcgagg
    12901 ccgagcaggg tgaccagcac ggcgagcccg tagccggcgc cggcacgcag gtccagatag
    12961 cgcgcggcgg cggcggcgac ctcggggcgg gccgcggcgg gctcgacgct gccggcgacg
    13021 acgttgcgga tgtcgttgag cagcagcgcg agctgggccg gcggctgttc gcgcagggct
    13081 tcgggcagcc cggcgatcac gagccgggtc agcagcccgc cttcgagcga gatccagatc
    13141 aggaacaggg ccagtgccgg cagcgcgcac cagagggcgg catgcaggcc gtagtagccg
    13201 ggcagcgaat gcagccggtt cacgcggccg ccggcgacgg tgaacgcgcg ccgccggccg
    13261 aggtgatagg cgagggccgt gaggccgagc aggagcgcca gagtcaggga tggtgtcatc
    13321 gtcggaaatc cgcggcgggc tggggaatgc cgtgtacggg ccgcactgcc gggcggggac
    13381 ggcgcaaggc ggccgggcgc gccatccgca ccccgccgcc tgcggcggac ctcacggcgc
    13441 tcgccggccg gcagcttact tgctgccgat cgtcttcagt tcgcgcgccg cggcagtgac
    13501 cgacttgcgc tcggcgtcgg gcagcgggat caggcccttg tcggcgagat agccgtcggt
    13561 gccgaaggac ttctcgctga cgaactcgtt gacgaactcc ttcaggccgg ggatcacgcc
    13621 gacgtgtgcc ttcttcacgt agaagaacag cgggcgcgcg atcggatact tgccggcggc
    13681 gatttcctcg aaggtcggcg agtggccctc gacgatcgag ccctgcacct tgtcgaggtt
    13741 ctcctccatg aagctgtagc cgaagatgcc gaaggcgctc ttgttggcga cgagcttctg
    13801 cacgatcagg ttgtcgttct cgccggcctc gatgaagctg ccgtcctcgc gcatcgtctg
    13861 gcagacggcc ttgaactgct tcggatcgcc gtccttcagc gccttgatcg ccgggaaggt
    13921 ttcgcagccg tggtccatga ccagctcgac gaacgcatcg cgcgtgcccg aggtcggcgg
    13981 cgggccgagc acctcgatct tggcgttcgg cagaccggcg tcgatctggc tccagttctg
    14041 gtacggattg gcgacgagct tcgtcgggtc cttcgggtcc ggcacggtct tggcgagcgc
    14101 gaggtagatc tgcgcacgcg tcagcttgac ccacggcgcg cccttggcgt tggcgaacac
    14161 gatgccgtcg tagccgatct tgacctcgac gatgtcggtg acgccgttct tcgcgcagct
    14221 ctcgatctcc gaggtcttga tcgcgcgcga ggcattggaa acgtccggat gctcggggcc
    14281 gacgccggcg cagaacagct tgaagccgcc gccggtgccg gtcgattcga ttttcggcgt
    14341 cttgaacttc gtgctcttgc cgaactgttc ggccacgacg gtcgtgaacg gatacacggt
    14401 cgaggaaccg acgacgctga tgtagtcgcg ggccgactgg gcctgcacga cggcagcggc
    14461 agcgaggctt gccgtcacgg cgagcgcgag aagcggcttg ttcacgaaaa cctccggtga
    14521 gacatgaatt tgtcagatcc gcgcgctggc ggagcaggag cttgatctga gggcgcggcg
    14581 cattctggcc gggcattgct acggtttcat gacagcctgc agggttttgt tgcgctgtga
    14641 tgacagcggg ctgccgtgcc ggcgcagccg gtcgggaccg gcggaaacag tgtgcggacc
    14701 gtggattgca gcgtgatgtc gatgcgcgtc agatcggatc gccgtcaagc tggcgttcca
    14761 gggcctcgcg caggcgcgtc tgggagagcg ggtcggcgag cggacgctgg gcgtcgtccg
    14821 tgatcaggaa gatgtcctcg gcgcgcgcgc cgatggttgc gatgcgggcg ttgtgcacgc
    14881 ggatgccgca gtgcatgaag gcgtggccga tgcggcagag caggccgggc cggtcggtgg
    14941 tgatcagcgt cagcaccgtg tgccgctgcg cggcgtcctc ggcgaagtag acggtggtcg
    15001 gcgtgcggaa atgccgaagc gcgcgcggcg cgcggcgcga gggcggcgcg agcggcgctt
    15061 ccgggtgctg cagctgccag cgcaggcgct gcatgatctc gcgcatgcgc gtccggccgc
    15121 tgatcggctc gccggtgtcc tcgagcaccg tgaagctgtc gagcgtgtag tcgccgaggc
    15181 tggtgatgat gcgcgcatcg agcacgttca ggttcagcgc atcgagcgcc gcggtcacac
    15241 gcgcgaacag gtccttctgg tcacgggcgt agatgaagat ctcggtgccg ccgcgccgct
    15301 cgtccttgcg cgcgagcacc agcaccagcg gccggccgtc gtcgcgcttc ttcagcaccg
    15361 cgcgcgtgtg ccaggcgatc tcgtcgggtg cgtagcgcag gaagtagtca tcgccgaact
    15421 gcgcccagac ctggtcgatg cgcgagtcgt cggtgatcgt gcgcccgagg atgcggcggg
    15481 cctcggcctg cacgtcgcgg atgtgttcct ccttgtcgag cggcgtgacc agtccgcggc
    15541 gcagcgcctg gcgcgtcgat tcgtagagat cgcgcagcag cgacgcgcgc cagtggttcc
    15601 acaggctcgg attggtggcg cggatgtcgg cgacggtcag caggtacagg taatcgaggt
    15661 gggtctggtc gccgacgcgc tgcgcgaagg cgtggatcac gtccgggtcc tggatgtcct
    15721 tcttctgcgc ggtcagcgac atgatcaggt gattgcgcac cagccacgcg accagattgg
    15781 tgtcgtagcg cgacaggccg tggcgctcgc agaaggcttc ggcatcgtcg gcgccgagca
    15841 ccgagtgatc gcccccgcga cccttggcga tgtcgtggta cagcccggcg atgtacagca
    15901 gctccggctt cggcagccgc cggcagaccg ccgtgcagtg cgggaattcg ttctcgaact
    15961 gcggcaggta gaagcggcgc aggttgcgga tcacgaacag cgtgtgctga tcgaccgtgt
    16021 aggtgtggaa caggtcgtgc tgcatgcgtc cggtgatcga accgaactcc ggcaggtagg
    16081 cggcgaggat gccgtacttg ttcatgcgcc gcagctgccg ggtcaggcgc ttgggctggc
    16141 gcatgatctc catgaacagc gcccgcgcgc gcaggtcgct gcggaagcgg tcatcgatca
    16201 gcgggcggtg cgcgcggatc agccggatgg tctcggcgcg cacgccgcgg atctcggcgt
    16261 gctgctgctg cagcaggaag acctccagca gcgccaccgg atagcgcagg aacacgttcg
    16321 ggcgcgtgac ctccagatag ccgttgcgca cctggaagcg gcggttcagc ggcaccggct
    16381 cggcgtcctc gtgcaggatc gcctcctcga agaactgcag cagcatctcg ttgagctgcg
    16441 acagcgccat caccgtgcgg tagtagtcct gcatgaagcg ctcgaccgcc agctggccgc
    16501 cgtgatcggc atagccgaac agcttggcga gcgtgcgctg gtggtcgaac agcagccggt
    16561 cctcgcgccg gccggtgatc gcgtgcaggc cgaagcgcac ctgccacagg aagtcctggt
    16621 attcgctcag ctcggtgaat tcggcgtcgg tcaggaagcc gtgggcgatc aggccgtgca
    16681 gggtccgggc accgaaatgg cgcttggcga cccagccgat catctgcagg tcgcgcaggc
    16741 ccccggggcc ttccttgacg ttcggttcga ggttgtactc ggtgtcgtgg aacttgcggt
    16801 agcgctgctg ctgctcggcg cgcttggcct cgaagaacgc cgccggcggc cagacgcggc
    16861 ccggggcgat gcgcgtgcgc atgtcggcgt acagcgcctc ggggccggtg atcagccgcg
    16921 cttccatcag gttggtcgcg accgtgatgt cggacgtgca ttcgcgcacg cagtcctcga
    16981 ccgtgcgcac gctctggccg acctcgatgc cgatgtccca caggaaggtc agcagctgct
    17041 cgatcgcggc gccggtgccg gcatcctcgg gcgcggcgag cagcagcatg atgtcgacat
    17101 cggagcccgg atgcagctcg ccacggccat agccgccgac ggcgagcagc gcgagatcgg
    17161 tgcgcgcctc gccgatgaaa tggcgccaca gcagcgccag cagccggtcg acgaggcgcg
    17221 cgcgcagcgg caccagcacg cgtgcgtgca cgccctggcg gaagcgttcg cgcagccggg
    17281 tgtcggcggc gcgcagcgcc tcgcggaagg cgcgcaccgg cggcggcccg gcggcggcga
    17341 gggctgcgcc aagtgctgcc agatcgagca gttcggtgcc agccgccgcg gcggcaacgg
    17401 aatcggggct ggcgggcatg gcgcctccgg aagcgttcag agcggatcgc cggggcgcac
    17461 ggtcagcacg tcgacaccgt gctcggtcac ggccaccgtg tgttcccact gcgccgacag
    17521 gctgtgatcc ttggtgacca cggtccagcc gtcgggcagc agcttcacgt ggcgcttgcc
    17581 ggcgttgacc atcggttcga tcgtgaaggt catgccgggc acgagttcgg ggccgctgcc
    17641 gggcttgccg tagtgcagca cctgcgggtc ctcgtggaac acgcggccga tgccgtggcc
    17701 gcagtattcc tgcacgatcg aacagccgtt ggcctcggcg tggcgctgga tcgcatggcc
    17761 gatgtcaccg aggcgcacgc ccgggcgcac catctcgatg ccgatcagca tgcattcctt
    17821 ggaaatgcgg cagacgcgct tcgcgatcac cggcacctcg ccgatctcga acatgcggct
    17881 ggtatcgccg tgccagccgt ccttgatgac ggtgacatcg acattgacga tgtcgccgcg
    17941 cttgagtttc ttcgggcccg ggatgccgtg gcagacgacg tgattgacgg atgtgcagat
    18001 cgacttcgga aagccgcggt aattcagcgg cgccgggatc gcctgctgca cattgaccgt
    18061 gtactcgtgg cagaggcggt cgagctcctc ggtcgtgacg ccctcgcgca cgtgcggttc
    18121 gatcatcgcc agcagttcgg ccgccagacg gccggcgacg cgcatctgtt cgatctcttc
    18181 gggcgtcttg atggaaatgg ccatgcaggt ctcgacggcg ggagggcggg gcggcagcag
    18241 cggcgcgagg cggtgcgggc cggcggacgg cgaccgggcg ctgttgggtg aaaagaccgc
    18301 gtatggtata aagccgccgc cccgcgggca attgcatgcg gggccaacct tccgaagtac
    18361 aacccgagac tactcacacg tgaccggcac gtccggcggg gtgccctttt cgtcgtccgg
    18421 acgaacgggt cgccgggcgg ggcgcgtgga ggctcaaccc cgaacttcgt tccaaggagt
    18481 cccgatgtcc accgtttcga tgcgcgacat gctggaggcc ggcgtccact tcggccacca
    18541 gacccgttac tggaacccga agatgggtgc ctacatcttc ggcgcgcgca gcaagatcca
    18601 catcgtcaac ctcgaaaaga cgctgccgct gttcaacgac gcgatgaact tcgtcggcaa
    18661 gctcgccggc aacggcggcc gcatcctgtt cgtcggcacc aagcgctcgg cgcgcacggc
    18721 cgtggccgaa gccgcggcgc gctgcggcat gccgtacgtc aaccaccgct ggctcggcgg
    18781 catgctgacc aacttcaaga ccgtgcgcca gtcgatcaag cgcctgaagg atctcgagca
    18841 gatgagcgcc gacggctcct tcgagcgcct gtcgaagaag gaagtcatcc tgctgcgccg
    18901 cgagatggaa aagctcgagc gcagcctcgg cggcatcaag gacatgccgg cgctgccgga
    18961 tgcgctgttc gtcatcgacg tcggtcacga gaagatcgcg atccaggaag ccggcaagct
    19021 cggcatcccg gtcgtcggca tcgtcgacac caacaattcg cccgatggcg tcgattacgt
    19081 gatccccggc aacgatgacg cgatccgcgc gatcacgctg tacgccgacg gcgccgccgc
    19141 cgccgtgatg accgcccgcg gcgcgctgcc ggttgccgcc cgcgccgacg aggacttcgt
    19201 cgaggtcccg gccgagcgga ccgccggctg atccgtcccg ttccggccgc aggcccgcga
    19261 gcccaaggca agcgggcctt tttgcgcgtg ccgtccgggc atgcgctgca tctttcagat
    19321 gaagcaaaga ggaatccact gacatgtcca tttccgcagc catggtcaag gaactgcgcg
    19381 agcgtaccgg ctcgggcatg atggaatgca agaaggcgct gaccgagacc ggcggcgaca
    19441 tcgaggcggc ggtcgagctg atgcgcaagc aggggctcgc caaggccgac aagaaggccg
    19501 ggcgcaccgc tgccgagggc acgctggtgg tcgccgccgg tgacaaggcc gtcgcgctgg
    19561 tcgaggtcaa ctgcgagacc gacttcgtcg ccaagggcga cgagttccag ggcttcgccg
    19621 ctgcggtcgc cgcgagcgcg ctcaccgcca gcccggccga tctcgacgca ctgctggccg
    19681 cgccgctcgc cgatggcaag tcggtcgacg agatccgccg cgagaaggtc gcgaagatcg
    19741 gcgagaacat cgccgtgcgc cgcttcgtgc gctacgagag cgccggcggc gtgctcgcct
    19801 cctacctgca cggcacgcgc atcggcgtgc tggtcgaact cgccggtggc gacgaggaac
    19861 tggcgaagga cgtggcgatg cacatcgccg cgagccgccc gctgtgcgtg tcgcccgagc
    19921 aggtcccggc cgaggtcgtc gagaaggagc gcgagatcgc gctcgccaac gccgtcaacg
    19981 aaggcaagag ccccgagatc gccgcgaaga tggtcgaggg tcgcgtgcgc aagttcctga
    20041 acgagaacac gctgctcggc caggtgttca tcaaggatcc ggaaggcaag cagaccgtcg
    20101 ccgcgctgct caagggccgc ggtgccagcg tcgtcgcgtt cacgcgctat gaactcggcg
    20161 aaggcatcga gaagaagcag gagaacttcg ccgacgaggt catggcccag gtgcgcggca
    20221 gctgagggac cgtgcgggag cccgctgttg tcgcagtgca ccacaaacgc gcgggcacgg
    20281 cgggctcctg atacattaac ggtcccccga atccaacgat ggactttccc atgccggacg
    20341 ccagacccgc ctacaagcgc atcctcctga aactcagcgg cgaagccctg atgggcgagg
    20401 gcgatttcgg catcgatccg ggcgtcatcc accgcatcgc caacgaggtc ggccaggtca
    20461 accgcatggg cgtcgaggtc ggcatggtga tcgggggcgg caacctgttc cgcggtgccg
    20521 gcctcgccga ggccggcatg gatcgcgtca ccggtgacca catgggcatg ctggcgacgg
    20581 tcatgaactc gctggcgatg caggatgcgc tggagcgcat cggcgtgttc gcgcgcgtga
    20641 tgtcggccgt gcgcatcaac caggtgtgcg aggactacat ccgccgccgc gcgatccgcc
    20701 atctcgagaa gggtcgggtc gtgatcttcg ccgccggcac cggcaacccg ttcttcacga
    20761 ccgattcggc cgcgagcctg cgcgcgatcg agatcaacgc cgaggtgatg ctgaaggcga
    20821 ccaaggtcga cggcatctac acggccgatc cgaagaagga ccccaccgcg acgcgctatg
    20881 acacgatcac ctatgacgag gccatcaccc gcaagctgca ggtcatggac acgaccgcga
    20941 tcgtcatgtg ccgcgagcat tcgatgccgc tgaaggtgtt cgacctcaac cgccccgacg
    21001 agatcgtgcg cgtggtctgc ggcgtgccgg gcatcgccac caccgtggtc cgaggctgag
    21061 actgacagat gatcaacgac atcaagaagg acgccgcatc gcgcatggag aaatccgtcg
    21121 acgcgctgcg ccatgaactc gccaagctgc gcaccgggcg tgcgcacgtg agcctgctcg
    21181 atcacgtgca cgtcgagtac tacggtaaca gcgtgcccct gtcgcaggcg gcgtcgatca
    21241 acgtctccga tgcgcgcacg ctggtcgtgc agccctggga gaagaccatg gtcggcccga
    21301 tcgagaaggc gatcatgacc tccgacctcg gcctgaatcc ggtcacggtc ggcaccgtga
    21361 tccgcgtgcc gctgccggcc ctgaccgagg agcgccggcg cgagatgggc aaggtcgtgc
    21421 ggcacgaagg cgaaagcgcc aaggtcgccg tgcgcaacat ccgccgcgac gccatcgccg
    21481 atctcaagat gctgcagaag gaaaaggaga tcagcgagga cgacgagcgt cgcggcaccg
    21541 acgacgtgca gaagatcacc gaccgcttcg tcgaggagat cgacaagctc gtcgccgcca
    21601 aggaagcgga gcttctggaa atctgaacgg tttttctccc ggccctcgac gcaggacatc
    21661 ctccttgagc agctccgaca gcacacccgg caccctgccc cggcacgtgg ccatcatcat
    21721 ggacggcaac ggccgctggg cgaagaaccg caagctgccg cgaccgttcg gccatcgcga
    21781 gggcgtcaag gcgctgcgtc gcgtcgtgca gcacggcatc gaccgcgggc tggatgccat
    21841 gacgctgttc gcgttctcca gcgagaactg gcgacgcccg gcgctggagg tcaagctgct
    21901 gatggagctg ttctcggtgg tgctgcgctc cgagatgaag cggctgatgg acaacaacgc
    21961 ccggctgcgc ttcgtcggcg accgcacgcc gttctcgccg cggctgcgcg aggccatgat
    22021 ccaggccgag gaaaccaccg gccgcaacac cggcatgacc gtcgtcatcg ccgccaacta
    22081 cggcgggcgc tgggacatcg ccaacgccgc gcgcgaactc gcggtcgagg tggccgccgg
    22141 acggctgcag cccgaggaca tcgatgccgg gctgatccag cgccacatca gcctcgccga
    22201 cctgccggag ccggatctct tcatccgcac cggcggcgag gagcgcatca gcaacttcct
    22261 gctctggcag atggcctaca ccgagttcta cttcaccgac tgcctgtggc ccgatttcga
    22321 cgaggccgcc ctcgatgccg ccttcgactc cttcggccgc cgccagcgcc gcttcgggcg
    22381 caccggcgac caggtggagc agcggggtgc ttagacagcg catcctgacc gcgctggtgc
    22441 tggccgcggt cgcgatcctc ggcgtgctgt ggctgccgag cggcgtgctg atgctgctgg
    22501 tcgccggcat cgtcgcgctc ggggcacgcg aatggctgca gctgatcggc ctgcccggtc
    22561 acggcgcgcg catcgccttc catggcgcgt ggctcgtgct gctgctgctc ggctggctcg
    22621 gcggcgagac cggcttcggt ctgctgctgg tgctgctcgc cgcgctgttc tggctgctgc
    22681 cggtgccgct gtggctgcgc cgctatgccg ccgaaccggc gctggcgcag ccgcgcgaat
    22741 ggtgggtgat cagcggcctc gtcgtgctcg ccggcggcgg cgtcgcgttc acggtgctgc
    22801 acgatgcgcc gggctacggg ccgggctggg tgctgctgct gatgttcctc gtcgccgggg
    22861 ccgacatcgg cgccttcttc gccggccggc gcttcggtcg cgtcaagctc gcgccgcaga
    22921 tcagtccggg caagacccgc gagggcgcct ggggcgcgct ggcggcgacg gcgctggtgg
    22981 cgctggccgg tgccgcggcg ctcggcgtgc cggccgcgcg ctggccggcc ttcctgctgg
    23041 tctgcgcgct gaccgtgatc tggtcgatcc tcggggacct gttcgaatcc atgctcaagc
    23101 gccagcacgg cgccaaggac agcggcacgc tgctgcccgg tcacggcggc gtgctcgatc
    23161 gcatcgacag cctgaccgcc gccgcgccgg tgttcctgct cggcctcacc gtgttcggac
    23221 cctggcgatg agcgcgcgcc cgctggcggt gacggtgctc ggcgccaccg gctcgatcgg
    23281 cctgagcacg ctcgacgtgc tggcccggca tccggatcgc taccgcgtgc atgcgctgag
    23341 cgcgcaccgc aacgtcgacg cgctcgccgg gctctgcgag cggcaccggc cggagtacgc
    23401 cgtgctgccg gaatcgtccg cggcatcccg gctgcaggcg cggctgcacg ccgccggctg
    23461 ccgcaccgag gtgctggccg gggccgaggc gctcgccgcg gttgccgcgg cgccggaaac
    23521 cgatgcggtg atggccgcga tcgtcggggc cgccggcctg ctgccggcgc tcgccgccgc
    23581 gcgtgccggc aagcgcgtgc tgctcgccaa caaggaagca ctggtgatgt ccggcgcgct
    23641 gttcatggag gcggtgcggg cacacggtgc gcaactgctg ccgatcgaca gcgagcacaa
    23701 cgccatcttc cagtgcctgc cgccggccgc gcaggcaggg gggcggcccg aaggcgtcac
    23761 gcgcctgctg ctgaccgctt ccggcggacc gtttcgcgac acgccggtcg acgagatccc
    23821 gcacaagacc ccggccgagg cctgcgccca tccgaactgg tcgatgggcc gcaagatctc
    23881 ggtcgattcg gcgacgatga tgaacaaggg cctcgaactg atcgaggcct tctggctgtt
    23941 cggcctgccg cgcgaacgca tcgaggtggt gatccatccg cagagcgtga tccattcgat
    24001 ggtcgaatac gccgatggct cggtgcttgc gcagctcggc aaccccgaca tgcgcacgcc
    24061 gatcgcccac gcgctggcct ggcccgagcg catcgcctcg ggggttgcgc cgctcgacct
    24121 gttccggatc ggccggctcg acttccgcgc gccggacctg ctgcgctatc cctgtctcgg
    24181 gctcgcgcag gccgcgctcg cggctggcgg cacggcgccg gcgatcctga acgccgccaa
    24241 cgaggttgcg gtcgcagcct tcctcgacgg ccggctcggc ttcggcggca tcgcgcagct
    24301 gaacgctgcc gtgctcgatg cggtgccggc cgcggccgcc gacacgctcg atgccgtgct
    24361 cgccgccgac gcggcggcgc ggcgtgcggc cgaggcgcga atcgacggcg acaagctcgc
    24421 aggaggtgtc cgatgaacgg tccgctgctg tcgttcctgg cgctggtggt cacgctcggc
    24481 gtgctgatca ccgtgcatga gttcggtcat ttctgggtcg cccgccgctg cggcgtgcgc
    24541 gtgctgcgct tcgcgatcgg cttcggccgg ccgctgtggc gacgcaccgc ccgcgacggc
    24601 gtcgaatacg tgatcggtgc cgtgccgctc ggcggctacg tgcgcatgct cgacgagcgc
    24661 gagggcgagg tgccgccgga gcagctcgac caggccttca accgccagac ggtcggccgg
    24721 cgcgcggcga tcgtcgccgc cggcccggcc ttcaacttcg cgttcgcgat cctcgcctac
    24781 ctgatcctgt acctcagcgg ggtcagccag gtgcgcccgg tgctcgccga ggccgcagcc
    24841 ggcacgccgg ccgcgatcgc cggcatccgc gacggcgacg aggtgctggc ggtcggggac
    24901 gtgacggtgc agggcctcga tgaactgctg atgcgcgtgg ccgagcagat ggagcccgga
    24961 gaggcgctga agctgcgcgt gcgcgatgcc gacggtcgtg tgctcgaacg ccgcctgccg
    25021 ctgccggagg acctcgacac cgatggcaac ctgctggccc ggctcgggct gcagccgtgg
    25081 cgaccgtcgc tgccggcggt gatcggcagc atcaagcccg acagcccggc ggcagcggcg
    25141 gggctgcagg acggcgatcg catcgtcagc gtcgatgacc agcgcgtcgc ggactggcag
    25201 gacttcgcca cgcgcgtgcg cgcgaagccg gaagccgacc tgcgcctgca gatcgagcgc
    25261 gacggcagtc cgctcagcgt gcgcctgcgc ccggtccgca tcgagaccgc gcagggggcc
    25321 gtcggccaga tcggcgcctt tgcggcagtg ccgcagtcgc tcgccgactc cctgcgcgtc
    25381 gtggtacggt atggcccgat cgatgcagcg gttgcagccg tcgagcgcac ctgggaaatg
    25441 tcggcgttca cgctgcgcat gctcgggcgg atgctgacgg ggcgtgcctc gctggacaac
    25501 ctcagcgggc cgctgaccat cgcgcagatg gccgggcagt cggcatcgat cggtctgtcc
    25561 gccttcctgt cgttcctggc cctggtcagc atcagcctcg gtgtgctgaa tctgctgccg
    25621 gtgccggtgc tcgatggcgg gcatctggcg tggtatgcca tcgaaacgat caagggcagc
    25681 ccggtttccg aggctgtcca gagcgccggg cagcgggtcg ggctggccgt gctgctggtg
    25741 atgatgggcc tggctttcta caacgatctg gcgcggttgt tcgggccctg agcaaagggc
    25801 gccggacggc tgcggtcggg tctgccgtcc gtgcgatcca tgactccaat cctgcgtaac
    25861 ttcctgctgt ccgccagtct cgccgcgccc gcctgcatcg tgcaggctgc ggaattcatc
    25921 gtgcgcgaca tccgtgtcga gggcctgcag cgcatctcgg cgggcacggt gttcagctac
    25981 ctgccggtgc agaccggcca gcgcgtgcgt caggaggatt tcccggatct gatccgcacg
    26041 ctgttccgca ccggcttctt cacggacgtc tcgctgcagc gcgacggcga cgtgctggtg
    26101 gtcaacgtcg tcgagcgccc ggcgatcgcc gaggtcaagg tcaccggcaa caaggacatc
    26161 tcgaccgagg atctcgagaa gtccctgaag acggtcggtc tcgccgaagg tcgcaccttc
    26221 gaccgggcgc tgctcgacaa ggtcgagcag gaactcaacc gccagtacta cgcgcgcggc
    26281 aagtacgcgg tgaaggtcga cagccaggtg cgaccgctcg aacgcaaccg cgtggcgatc
    26341 acgatcgaca tctccgaagg catcgcggcg cgcatcaagc agatcaacat cgtcggcgcc
    26401 aaggcctttg atgaggacga cctgctcgac ctgttccaga gttcgaccag caactggacc
    26461 tcgctgctgt cgaaggacga ccagtactcg aagcagaagc tcggcggcga tctcgaaacc
    26521 ctgcgctcgt attacctcga ccgcggctac ctgcgcttca acatcgattc gacgcaggtc
    26581 acgatcacgc cggacaagaa ggacatctac atcacgatca acatcgccga gggcgatcgc
    26641 tacacggtca aggacgtgcg cctcgccggt gaaagcccgg tgccgctcga tcagctgaaa
    26701 ccactggtca agctgcagcc cggccagacc ttctcgcgcg cgacgctcaa cgagagcacc
    26761 aaggcgatca ccgaccggct cggcgatgac ggctatgcct tcgccaacgt cagtgccgtg
    26821 ccgcagatcg acgatgccgg caagaccgtg tcgctggccc tgaacgtcga tgccggccgg
    26881 cgcgtgtacg tgcgccgcat caacttcaag ggcaacatca agacccagga cgaggtgctg
    26941 cgccgcgaga tgcgccagac cgagggctcg tggtttgcga cccgcgatgt caatcgatcc
    27001 aaggagcgtc tggagcgtct ggcctttgtg caggaggtca cggtcgaaac tccggccgta
    27061 cccggcacgc cggatcaggt cgatctgaat tacagcgtca ccgagcgccc ggccggcagc
    27121 ctgagcttcg gcatcggcta cggccaggat gccggcgtgc tgctcagtgc cagtgtctcg
    27181 cagcagaact tcctcggcac cggcaacgcg ctgagcttca cgttcaacaa cagccagatc
    27241 aacacgatct acagcttcag ctacacgaat ccgtacttca cgcccgatgg cgtcagtgcc
    27301 ggcatccggg ccagctaccg tgagaccgac gcttccgaag ccaacctcgc gagctacctg
    27361 accaacgaat tcgagacgat ggcgaccttc ggcttcccgc tgaacgagac cgacaccgtc
    27421 aacctcgcga tcggcatctc cggcatcaag ctcaagacca acggcgacaa cacgccgcag
    27481 ggcatctacg actacatcga cgagcatggc gagcgccacg tcggtccgcg cttcgaactg
    27541 agctgggcac gcgacacgcg caacaaggcc gtcttcgccg accgcggctc gtacaaccgc
    27601 ctgtcgatcg aatcgatgct cggtggcgac gacagctggt acaagctcgg ttaccggcac
    27661 agcacctatc tgccgctgac cgattcgctg acgctgctcg gccatttcga cttcggctac
    27721 ggtgagggtt tcggcaatac cgacacgctg ccgttctacg agaacttcta cgccggcggc
    27781 ctgcgcaccg tgcgcggcta caagaccaac acgctcggcc cgcgctggga cgatccgaat
    27841 ccggacctgc gtgacccgaa gggtggtgcg ctgcgcacgg ccggcggggt cgaggtgctg
    27901 ttcccggcgc cgttcttcaa ggacaacaag aacctgcgct tctcgacctt cttcgacatc
    27961 ggcaacgtgt atgcggaagt gaacgacttc gatgccggcg aactgcgcta ttccgccggc
    28021 ttgtcgatgc agtggatcac gccgctcgga ccgctggtga tgtcggtcgc ccagccgctc
    28081 aacaagaagg acggcgacaa gaccgagccg ttccagttca ccttcggcgt gccgttctga
    28141 cggcccgcgt cgacccgagg ggaagatcgt cttgaaggcc ttgctgcgtg tccttctggg
    28201 ggctctcgcg ctgacgttcg cgctgccggc agtggctgcg gagctgaagc tcggtttcgt
    28261 caacgccgcg cgtgtgctga agagcgcacc gcaggccgag gcggcgctga accggctcga
    28321 aaaggagttc tcgccgcgcg acaaggcgct ggtcgaggcg cagaaggcgc tgcgcaagct
    28381 ggaggacagg ctctccaagg acggcgccac gctcagcgac gccgaacggc gcgacctcga
    28441 acgcgacatc atctcgcagc ggcgggatgt gaagcgcgcg caggacgagt tccgcgagga
    28501 cttcaacatc cggcgcaacg aggaaatggc caagctgcag aagctgatcc aggacgcgat
    28561 caccgcgctc gcccaggagg aacgctacga cctggtgctc aacgacggcg cggtgatctt
    28621 cgcgagcgag gctgtcgaca tcaccgacaa ggtgctgaag cgcctgtcgg ccgtgcacgg
    28681 caactgagcc ggtccggggt cgacggggtg catacgctcg cggaactggc gcaggtcgcc
    28741 ggcgcgcaac tgcgcggtga cgcggcgacg ccgatcgatg gcgtcggcac gctcgccgcg
    28801 gcccggcccg ggcagatcgg cttcctcgcc aatccgaagt accggcagct cctgtccggc
    28861 acgcaggcga gcgcggtgat cctgggggct gaggatgccg gggcctgtcc ggtcgcctgc
    28921 ctgatcgccg acaacccgca tctcgcctat gcccgggtcg cggcgctgtt cgatccggcc
    28981 gccggcaggc agcagggcgt gcatgcgcgg gcctgcgtcg atgcggccgc cgaagtcgcc
    29041 gacgatgcct ggatcggccc gatggccgtc atcgaggccg gggccgtgat cgggccgcgc
    29101 gtgcaggtcg ggccgggctg cgtggtcggg gcgcgcacga gcatcggtgc cgacagccgg
    29161 ctggtcgcca acgtcacggt gctgcacgac tgcgtgctcg gcgagcgcgt gctcgtgcat
    29221 ccgggcgcgg tgatcggcgc cgacggtttc gggctcgcga atgaccgtgg ctgctgggtc
    29281 aaggtgccgc agctcgggcg cgtcgtgatc ggcaacgacg tcgagatcgg cgtcaacacc
    29341 gccgtcgacc gtggcgcgct cgaggacacc gtgctcgccg acggcgtcaa gctcgacaac
    29401 ctgatccaga tcgcccacaa cgtccgcgtc ggcgcacaca cggcgatggc cggctgcgtc
    29461 ggcatcgccg gcagcacgac ggtcggcgcg cactgcacgc tcgccggagg cgtcggtctg
    29521 gccgggcatc tgacgctcgg cgaccacgtc cacgtcaccg gcatgtcgct ggtcaccaaa
    29581 tccctgaccg agcccggcgt ctatgcgtcc ggactcgcgg tcgagccggc ccggctgtgg
    29641 aacaaggttt ccgcgcgcct gcgccggctc gacgaactgt tccgccggct cgccgcgctc
    29701 gaaaaacgtg tcggcgacga tgccggtcac ccctagagga ccccgccctt ggaatcgatg
    29761 gatatcacgc aggtgctgcg ttatctgccg caccgctatc cgtttctgct gatcgaccgc
    29821 gtgctcgaac tcgagcccgg cgaacgcctg ctcggcctga agaacgtctc gttcaacgaa
    29881 cccttcttcc agggccattt cccgcagcag ccggtgatgc cgggcgtgct gatcctggaa
    29941 gcactcgcgc aggccaccgg catcctcgcc ttcaagacct ccggccagct gcccgaggac
    30001 gattcgacct attacttcgt cggcatcgac aatgcccgct tcaagcagcc ggtctgtccg
    30061 ggcgaccggc tgctgctcga cgtgcgcctg ctcaagaccc gccgcggcat ctgggtgttc
    30121 tcggccgagg cccgcgtcga tgacaaggtg gtgtgctcgg ccgaactgat gtccacgaag
    30181 cgggagcacg gccagtgatc gacccgcgcg ccatcgtcga tccgggggcc cgcatcgccg
    30241 aaggcgtgca gatcgggccg ttcagcatca tcggtgccgg tgtcgagatc gagaccggca
    30301 ccgtgatcgg cccgcacgtc gtgatcaagg gaccgacacg catcgggcgc gacaaccgca
    30361 tctaccagtt cgcgtcgatc ggcgacgatc cgcaggacaa gaaatacgcc ggtgagccga
    30421 cgcggctgga aatcggcgac ggcaacacga tccgcgaata cgtgacgatc aaccgcggca
    30481 cggtgcagga cgccggggtc acgcgcgtcg gccatgacaa ctggatcatg gcctacgtgc
    30541 acatcgcgca cgactgcgtg gtcggggacc acacgatctt cgcgaacaac gcctcgctcg
    30601 ccggacacgt gcacgtcggc gactgggcga tcctcggcgg ctacacgctg gtgcaccagt
    30661 tctgccgcat cggtgcgcac agcttctgcg gcttcgcgac cggcgtgcag cgcgacgtgc
    30721 cgccgtacgt gaccgtcgcc ggctatcgcg ccgagcctta cggcatcaac agcgagggcc
    30781 tgcgccgccg cggtttcgag gccgagacca tcgccgtgat caagcgcgcc tacaagctgc
    30841 tgtacatgtc ggatctgaag ctcgccgagg ccgtcgagcg catgcgcgag ctggtcgcca
    30901 cgcatccgcc ggtggcgctg ctcgccgatt tcgtcgccga ttcgccgcgc ggcatcgtgc
    30961 gctgacgcgc atgcgcatcg cagtcgtcgc cggggagctc tcgggcgatg cgcttggcgg
    31021 cgcgctgctg ccggcgttgc agcagcgttt tccgcaggcg cgcttcgagg gcatcggcgg
    31081 gccgcgcatg caggcggccg gcctcgaatc gctgttcccg ctcgaaaaac tgtcggtgat
    31141 ggggctggtc gaggtgctga agcacctgcc ggagctgctg tcgatccgcc gcgcgctcgt
    31201 gcgccgctgg ctcgccgagc cgcccgccct gtacctcggc atcgacgcac ccgatttcac
    31261 gctgccgctc gcgaagcgtc tgcgcgcgca cggcatccgc actgcgcatt acgtcagccc
    31321 ctcggtctgg gcctggcggc aggggcgggt gcgcggcatc gcgcagagcg tcgatctgat
    31381 gctgacgctg tttccgttcg aggcgacg
//
LOCUS       DAOQIE010000007        30247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_642_length_30247_cov_8.004074, whole genome shotgun sequence.
ACCESSION   DAOQIE010000007 DAOQIE010000000
VERSION     DAOQIE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 30247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30247
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_642_length_30247_cov_8.004074"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PK144_00915"
     CDS             complement(<1..1471)
                     /locus_tag="PK144_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF64310.1"
                     /translation="MNRSRLQPLALALVFALGGPPAVAGTATEELHAGLQSLDRNDYA
                     GAEIHLKNALQQRPDLAPAQVALGHTYLRQGRFELAESTLQGALRLGAERADIDPLRM
                     YLKLRQGEFQAVLDGFDPYRHTGAARARMLRLRGEARLELGLMEEAALSFTAAREAAP
                     QAPDGDIGLSMLALQQQDAALARSAAQRATGLDPRSTEAWTALAAAELAAGHATEALA
                     AADTALKLDELCLPARAARVSALIALGRADEAQPVLDRLRRQNPADVRTLLLAAELAR
                     ARQDPETARARLTDASLLLKAIPRETLLRSPSLLKLSGTVHEALGLSEKARGDLTQYL
                     ERMPGDETARRLLAAVLLRLGDPRQALELLQPLPDDPHAVSDVRLLTLLADAHTQLGQ
                     HATAAALLERLIELGGQQPALFAQLGQARLQSGQGDAALAALSAAWAQSGEARDGLLL
                     ASLQLGAGRPAEALRTLDAVAVRAPDHAVVLHLRGGARLA"
     gene            complement(1562..2647)
                     /locus_tag="PK144_00920"
     CDS             complement(1562..2647)
                     /locus_tag="PK144_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF64311.1"
                     /translation="MNQRMLAAAIGAACLIGGVPTTQAAFFLPTPQFNAFQYGDFYSY
                     SLPILAYRYAREFGGGVGPGNPFYIASGPGQISDLTVIGTGANGQPVNTNYSGVDNAY
                     QTPNNTGVDQFQTTTSAPGGVDDPGDNGNPANHTTEFVGDGENTWDARVSALTSFLNG
                     GDLLFFFNNNQVNSGAATNQNLMAWGQFTLKDDAGVAPDLVFDLTNNRGAGGNPSLTG
                     AETLGYTHGAGDTPALNAAPASITTTDFYYILSGGQVTIPSSDTCRSIFGYQIPGCVP
                     DADQTFNHNLGANQAAYMLYSPELNTAIANGLYDVMQFDFRMSALNNGYEQIFIASSS
                     SVTSVPEPASLALLGLTLPWLMRRRRR"
     gene            2977..3993
                     /locus_tag="PK144_00925"
     CDS             2977..3993
                     /locus_tag="PK144_00925"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNF64312.1"
                     /translation="MPYRLLRPLLFRLDAERAHDLTLAALGRIGPAAGLMTRAVPRRP
                     RVVMGIEFPNPVGLAAGLDKDGSAIRGLAALGFGCLEIGTVTPRPQAGNPRPRLFRLP
                     EAQALINRYGFNNGGVTALVNRVMAARAAGFRGVLGINIGKNKDTPNERASDDYLLCL
                     QQVYAHADYVAVNISSPNTPGLRDLQYGEALDRLLDELKEAQTRLADHHGRYVPLALK
                     IAPDVADADLPVIAAALVRARLDAVIATNTTFSRAGVEHLRHAGETGGLSGAPLRERS
                     TAVIAGLAKALDGALPVIGVGGILSGDDARAKIEAGASLVQLYSGFIYRGPALIREAV
                     EAIG"
     gene            complement(4025..4669)
                     /gene="rnt"
                     /locus_tag="PK144_00930"
     CDS             complement(4025..4669)
                     /gene="rnt"
                     /locus_tag="PK144_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNF64313.1"
                     /translation="MNDSVRKSPIAFRFRGFLPVVVDIETGGFNARTDALLEIAAVLL
                     RLGSDGRLYPARTIAHHVEPFAGANLDPASLKVNGIDPFHPFRLALPEAEALGNVLRE
                     VRREVRETGCTRAIMVAHNPAFDLGFLNAAAERCDVKRNPFHPFSTFDTVTLAGLAYG
                     QTVLARAAQAAGIAWDAREAHSAVYDAERTAELFCNIVNRWKDLGGWVPSADDP"
     gene            4871..9727
                     /locus_tag="PK144_00935"
     CDS             4871..9727
                     /locus_tag="PK144_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF64314.1"
                     /translation="MLRRRFFRNPVFANLALLLALLAGVLSWWQMPREQTARPSAAAV
                     EIVTAAPGLATAEIETRVTAVLEAAIKPIADLDFIASTSRVGESRLTVRLRTVSEATF
                     ERRLNELRWIVQHVERQLPSLAGRPEIASVHGAGVLPSADVLVLARAPDAPAPYAAAE
                     RLAQSFERLMDVERVERYGTRATRVEIRFDPERVARHGLTPARLAELVGAQLREQAVG
                     RVDFDERPMQLLVEGADLQPERLAQLPVPGVRGLQPLAALADIERGPEPATRAVLADG
                     RPAVLLSVYRKSDANALDLSAALGEQAARFADSDAEAAAIELRVLDARSTDTRRALQA
                     YERGALPALAAALLVFAIAFGWRLAMLVACVVPAAVGLALAPWGFSGGVLTPVMLTGV
                     LAGFVLLIDAAVAVGERLQHRLLRGAAALDAAYGAWREAGLPLMVATLVFLLAMLLPM
                     WLPEGSGPAGALAARVMTGMLVAGIAYAVLLLPSHAVVTRIRLPRRHPVQARREWLAW
                     RLRHAYRRLLNWTLRRPLVPGLVLVLLLLGALVALGSGRVGVEFAERGQLRSFYVNLE
                     LSPGAKLEDTLAAGREVERIVREQLPADALAEVVISAGLRRSERELLTGERHGQVLVG
                     LRSGDRIRDALTVEQAITRVKPRLAFVGGVDNLSVQAVDDGRPVARPLSVTVRGDDPA
                     EVRRAADRVVDILRSLIGVTDVVDDEDQAEAAELRLVLKPGALLRAGLSAPQLLRELR
                     LLGEGETIAQLREQGRNVDVLLRGKAAPEPFFDIEEFLARPVRLPDGRSAPLGEFASA
                     EPASGRSVVRHQNLRRAISIEADVDQARVNTFALNRVIATEWERTAAAGLPGVELVIG
                     GAFGSVAGHLGALVSLFALGSGLGYLVLATRAHSYRQPLVIFAAVPFAALAFVVGLAV
                     SGQPVGPYTGFAGTALLGLSVQSTLLLVSAVQRLLPIEGSVARAVALAAADRALPVLV
                     SALASVAGLAVFALGVPVAAPAWMALAGALIGGIPVIALCVLLVLPAICLYVLRAPVS
                     AVTAINVVDAEPGGGSWRERMRRGRRHAAIVALNESELNSAEPELRARHEAGLDALKV
                     GEWLQALRLFEDAAKRHPEAVQLQFGATQALLQYLFENEWDEGYHARAGRHLARARQL
                     ASGDPRLRPLERALAELEARHVPVAGEGTSSQPRVGMLRLSGTVGRPWRPASVARGQR
                     APDRGTDLPAGLHDLALALRNLVRQRKRTAAALGAIGFGAVALILAGGFIEWVLWATR
                     ESAVYGQLGHVQVVRQDFFVRGTADPRGFLIPGDAPPAGLRELPHVRAVTPRLSFSGL
                     ASTGETTVPYLGQGVVPEDEAPISIDYRIVSGKGLTTSDAREVIVGTGLAATLGVKPG
                     QKLILMSNSGSGAFTPVELKVRGLFSTSNKALNDVALRVPLKTAQDLLQVDGVHIWVV
                     LLDRTESTAAVLPQLRALLPAGLQAVPWSDLADFYNKTSELFQRQMFVLRLMIAVIIL
                     LSISNSLMMNVMERTGEIGTLCALGTRRRSILKIFSLEALALGLLGAATGTALGVTIA
                     SVASAVGIPMPPAPGMDKGFVAEILVTPALAGSTFGLAVIATFVSGLYPAWKASRLVI
                     VDALRHNR"
     gene            9741..10952
                     /locus_tag="PK144_00940"
     CDS             9741..10952
                     /locus_tag="PK144_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64315.1"
                     /translation="MLKFAYRNLTRHRGRTLLTLGAIAFGVVSLVLSAGFVDDIFVRL
                     RENTVQSGLGHLQVAREHYFDLRNRQPYAYMIENPGAVVAAAKKLPTVREAMMRVNLT
                     GTLSNGSGELPVVAEGGEPEKEVAVGGFVTLVDGRRLTGGDQNVVELGEGLARALGVA
                     PGAFVTLLTATPEGAINTVELKVVGIFRSVSIDYDARAARLPLATAQTLLNTKSVHTV
                     VVALENTAFTQTAAGQLRKALDGQPLAVYTWDELADFYRKAVDLYRTQFGVLQAIILI
                     MVALSVSNSINISIFERTAEVGTLLALGNRRRQVLQLLVMESAISGLVGGLAGAALGI
                     GLALGISSIGIPMPPPPNSSQGYLAVVRIVPEQVAVALVIGVVATVLASVLPAWRVSR
                     LPVVEALRRAQ"
     gene            complement(10988..11761)
                     /locus_tag="PK144_00945"
     CDS             complement(10988..11761)
                     /locus_tag="PK144_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNF64316.1"
                     /translation="MRATVLRLPSLLLLCAATLATPATRAQEVDLEALAMLEAADQVR
                     FPRSDFEVNVRISSTTPGGETEVRDYQILSKGNENTLVITTAPASERGQVLLMRGRDL
                     WVYSPRLSQPVRLPLSQRLTGQVANGDLARANFSGDYNARVLRDETIQKRKYAVLELT
                     AIDGSVTYNRVLLWVERSGNQPLKAEFYALSGRLLKTAQYLNYAKLDNTVRPTQLVME
                     DAVKRGDRSVLDYSAMKPRELPNKLFNKDYMKRVTQGID"
     gene            11856..12542
                     /locus_tag="PK144_00950"
     CDS             11856..12542
                     /locus_tag="PK144_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64317.1"
                     /translation="MSIVRIENVSKRYRLGEQDVTALHGVSLRVEPGEFVALAGPSGS
                     GKTTLLNMIGCIDTPSSGRVIVDGHDVTGRDPDQLAELRARTLGFIFQTFNLLPVLSA
                     EENVEYPLINIQHIPRAERRQRVAYFLDMVGLARFARHRPNQLSGGQRQRVAIARALA
                     TKPKIVLADEPTANLDHQSGDGILQLMKHINEQSGTTFIFSTHDARVMAMASRLVRIS
                     DGAIVGEETR"
     gene            12539..13840
                     /locus_tag="PK144_00955"
     CDS             12539..13840
                     /locus_tag="PK144_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64318.1"
                     /translation="MTNHPNRSGRIALGIALLAVTPAAAAQQDVKLSGYLSNETAYTF
                     GGENHWSKSRNTLNLAATGSFSESISWKASGWLRWDPVVQHSDFYPDKVQDDRGFEGL
                     VRETFLDVSLGDWDLRLGRQNIVWGEMVGLFFADVVSARDMREFVLADFEQLRIPQWA
                     ARAEYFKDDFHAELVWIPYLTYDNIGEPGDDFYPYPPAFPGLGYRILDEKKPRGGDSD
                     DAAGVRLGWIVDGWDINGYYYRSTDVQPAFQRSFGLVGGVPTLTYTPTHNRIQQWGLT
                     VGKDIGDGLVFKAEAIYTMGRRYDVLRLSDADGLVRKDTLDYVLGLDWTPDASNRLNL
                     QFFQRFYQDHDPDMVPESVESGVSLFYSTKVWGDRLEPEITLIESLNRGDWLLRPRLT
                     WNVSADLKASFGADILNGPPDALFGRFTNGDRVYAEVRYDF"
     gene            complement(13828..15861)
                     /locus_tag="PK144_00960"
     CDS             complement(13828..15861)
                     /locus_tag="PK144_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNF64319.1"
                     /translation="MSPQHVSQSFDYATAFSRNIGWLTPTEQAWLRGRRLAVAGLGGV
                     GGVHLLTLARLGVGAFHLADFDRFELHNFNRQAGATMATLDRPKLDVMAEAALAINPE
                     LDIRRFADGVTDDNLDAFFDGVDLYVDALDFFVLPVRRKVFAAAHARGIPAITAAPLG
                     MGTAVLTFLPGRMSFEDYFQVEGQPEEEQYARFLVGLSPTMLQQGYLVDRSTINFVEK
                     RGPSTPMACELAAGVLVTESLKLLLGRGPVRAAPWGLHFDAYRNRLSRTWRPGGNRHP
                     LNRLTLAIMRTLLRRARSLPAPARAAPAAPAGLDPRVARVLELASWAPSGDNTQPWRF
                     EAVDPLTVIVHGCDTRDHVIYDLRGRPSQLALGALLETARIAAPGAGLALDIETLPGV
                     GDTTPAFAPMFRLQFTPAAAAVRHPLETAIRARCTQRRPLSRRPLTDAVKAAFLQALG
                     PGYSLRWLEGDQRLDVAKLLFRNAHIRLTTPEAYPTHRDIIEFGVQYSEDRLPGAAVG
                     LDPVGLALMRWAMKSWERVDFLNRWFAGTWLPRLQLDFAPALGCAAHFLIIAEQAPQT
                     LADYLAAGAAMQRFWLTATRLGLQFQPEMTPLIFSGYIRDGIEFTTKQASVDNARTLA
                     RQLDALVGADTAARAVYFGRLGYGPVPVSRSTRLPLARLMTGGGSPDASPQKS"
     gene            complement(15930..16376)
                     /locus_tag="PK144_00965"
     CDS             complement(15930..16376)
                     /locus_tag="PK144_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNF64320.1"
                     /translation="MAVRIEIREHGFEPLAELAAHQQALDARLAGRYGATASFIGTMR
                     DHNEGDDVRGMRLEHYPGMTERELARLAEEAVERWSLLDALVIHRVGELLPNDPIVLV
                     AVWSAHRGAAFDACRWIMEALKSRAPFWKHETLPDGSRWVAKNTTG"
     gene            complement(16379..16615)
                     /locus_tag="PK144_00970"
     CDS             complement(16379..16615)
                     /locus_tag="PK144_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNF64321.1"
                     /translation="MPAIRILYFASLRERVGRASAELPGDDPLTVAEAWSRLNPALPL
                     PPNTLAAINHEYVDLGAPVRGGDELAFFPPVTGG"
     gene            complement(16636..17217)
                     /locus_tag="PK144_00975"
     CDS             complement(16636..17217)
                     /locus_tag="PK144_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="3'-5' exonuclease of DNA polymerase III; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF64322.1"
                     /translation="MLAALARPFRHAAPPEAFDFMQEAGGTAGELVCLDCAATSLDLR
                     SARLLSLAAVRIRGNRVLTSSALQLDIAGPEHGDGQGMSALEAVQKLLRFIGPRPVVG
                     YWLDFDLELLEPQIERLLGCPLPNATVEVSALYHARIRRQPRAPHRPLDLRFETLRRD
                     LELPALESGSPLAHAVLTALMYLKLSAAPDSQP"
     gene            complement(17217..19040)
                     /locus_tag="PK144_00980"
     CDS             complement(17217..19040)
                     /locus_tag="PK144_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNF64323.1"
                     /translation="MEPRPQLSRIPPFDRLPAETLARVAEAVDVLFFRRDQTVLTEQE
                     APDSLYVLTQGSVQVLAGGEVIARYGPEDCFDLEALLRRRSPGRIVAREDTVCLALPR
                     AEFLYLLAECPEFADPLFAELAGRTAGSRPVDTHGELAGFMIAKIGDAYVHPPLQVTA
                     ASSIHEAARAMRDNRATSLLVVREDGEIGICSGTDLRNAVVIERRSPDAPVGDIASYG
                     LVSLEEDDFLFNALLTMTRHSISRVVIRHDGAIRGVLEQVDLLSYLSNHSHLIAVQIE
                     RAGSREELAKASHELTRVIEALHGKGVKLRYIGQLLSELNKKLLGKLFTLIAPPELID
                     NACLVVMGSEGREEQILKTDQDNALLLRDGYTHPGLDAITQAFTEALVAFGYPPCPGN
                     IMVSNPYWTKPLGAFKDEIFNWIKRPSEDGFMHFAIFFDAMPVAGDTALLDEAKRYLH
                     QQLQSNSGFFAHFARATVSFETPLGFFQNFVTGKDGQHRDELDLKKGGIFPLTHGVRS
                     LALEHRLEPTNTLERIEALEAKGVLTPARAAELTEALTMMSTLRLTTGLRKLKAGLPQ
                     DNFVNPNELNKLERELLRDSFKIVNEFKKFITFHFKLNLVT"
     gene            complement(19144..20841)
                     /locus_tag="PK144_00985"
     CDS             complement(19144..20841)
                     /locus_tag="PK144_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNF64324.1"
                     /translation="MKKLLPASLFASLFLALPAFADAVKGPVEKQAINVTAIVMFLAF
                     VAATMGITYWAASRTKTAKDFYTGGGGITGFQNGLAIAGDYMSAASFLGISGLVFSSG
                     YDGLIYSIGFLVGWPIIMFVMAEQLRNLGKFTFADVASYRFGQTEIRTMAAFSSLVVV
                     AFYLIAQMVGAGKLIQILFGLDYNLAVVGVGVLMLIYVVFGGMTATTWVQIIKAVLLL
                     SGATFMALAALSHFGFSPEEMFKQAVAGHPKGDKIMGPGGLVTDPINAISLGLALMFG
                     TAGLPHILMRFFTVPNAKEARKSVFYATGFIGYFYILTFIIGFSAIVLLVQHPEFYKA
                     DALVNGAVDKAKLISGLNGGTNMVAIHLANAVGGNIFLGFISAVAFATILAVVSGLTL
                     AGASAISHDLYASVIAKGRSDEVTEMKVSRGATITLGVIAVVLGIIFEKQNVAFMVGL
                     AFAIAASANFPVLIMSVFWSKMTTRGAMIGGWIGLTVAVVLTVISKAIWVDVIGNAAP
                     LITLNNPAIISIPSAFIAIWLFSVTDKSKRAEQDLLGFEAQDVRCQTGIGAEGASGH"
     gene            complement(20841..21149)
                     /locus_tag="PK144_00990"
     CDS             complement(20841..21149)
                     /locus_tag="PK144_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNF64325.1"
                     /translation="MQPDVVRQVKSNPKYHDLVAKRSAFGWTLAVVMLVIYYGFILVI
                     AYSPTSLAAKVGAGATMSIGMPLGVAIIVSAFLLTGIYVARANSEFDRLTHEIVEAVK
                     "
     gene            complement(21368..24781)
                     /locus_tag="PK144_00995"
     CDS             complement(21368..24781)
                     /locus_tag="PK144_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64326.1"
                     /translation="MLSPLFPRLRRLALRLPLIAAAVLVATLTVYALLGAREQAVFYE
                     ETVARQNATLARQTASQAALALLRSSRETLERLALDTARDGDVDSVQILDTAGTLLAR
                     ADATPEGPRLRPSGSAILPPGDGLPHFDWLADTADGQRRAAAWAPVGNAARPLGWVRL
                     EVHMPPAAAIGNWLLTRALPLGLFAAALGALLLAVALRRPLSALNEATDFARELDSRR
                     GEQLDLRCGSIEVSELGEVLNQVSARLAASEAALAASGERLATLLRQAVDGIMTLDAE
                     GRIESFNTTAEHLFGYPARQAIGCHIGLLLPEVRAGETFRHRELQGRRRDGALIPIEV
                     SVGSAHAEGSPQAMYIAIVRDISERRLAEQALGESEARKSAILQAALDAVITIDRRGR
                     VLEFNPAAERLFGFRRGSVLGRDLAELIVPPRLRERHRQGLERYLGTGVSDMLGRRFE
                     VDGLRADGSEIPVEIAVTPIHLEAGTIFTAYLRDISERRAAERALRDSEARYRRVVDS
                     VHEVIFQTDAEGRWAFLNRAWTEITGRNLDESLGRTVSECLDAEDRRSFRAQFALATP
                     DQPQGRSELRLLGRHGEPRWVLMSWELSHGPDGRPLGATGTISDIGERKAAEAELHRA
                     VEAAEAASRAKSEFLATMSHELRTPLNAVIGMTDLALDAVAEAEVRDYLVTARGAAGN
                     LLAIIDDLLDFSKLEAGRLVTRTEPFLLRDTLRTTITNHARAAAGRGLELLWEVDADV
                     PDAVTGDGERLAQIVSHLLSNAVKFTEQGEIVVRVRAQEVSEQSVVLHCSVRDTGIGI
                     APDKIARIFQPFVQGDTSSTRRFGGTGLGLSISQRLAEMMGGRLWVESSPERGSDFQF
                     TLRLERQPARPDVRRGPEWTGMRALVLAASQSARAVLERQLRYWGFQVAAAGVADEAE
                     RLLQERAGFGQHFELALFDETQPGTVELLRRIAPAERRPRVLLLRSPLTPPSASLPAG
                     ADASLLKPVDPARLAEALTRALSPAAERTPLVRFGDDATVTPAPRLDREAIDGRLAPF
                     DPAALLAQCHGNAALAGRLLDLLRRQIPEHLATLRRAVDSEDLVLIRREVRTLAGPLG
                     SVGAQAALSRLRSLEAADAAHAPACFQLLETELQRVLEFAALPLPAEAG"
     gene            complement(24906..27731)
                     /gene="rapA"
                     /locus_tag="PK144_01000"
     CDS             complement(24906..27731)
                     /gene="rapA"
                     /locus_tag="PK144_01000"
                     /inference="COORDINATES: protein motif:HMM:NF003426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HNF64327.1"
                     /translation="MNDFVGGSRWISETEPELGLGMVLSGDARRVTLLFHGSGETRVY
                     ARQGAPLTRVRFQPGDTVQSHEGWTLQVEEVLEERGLFAYVGRDGRVLPETGLSNFIS
                     FSKPQERLFANQTDAQRLFTLRCDALAWAAQARASELRGLAGARIDLLAHQLWIAHEV
                     AGRYAPRVLLADEVGLGKTVEAGLILHRQLVTGRAGRALIVVPEPLIHQWFLELRRRF
                     QLGCAVYDEERCLELEREGLDNPFLAEQLVLCGRDWLVGRERRQTQALEAGWDLLIVD
                     EAHHLAWEPGAPSPEYTAIERLAAHAQGLLLLTATPEQLGPNGHYARLRLLDPERFPD
                     LDRFRHEALAYRKVARIVDDLLAYRPLDQADLDELGACLGPETGDLMLERLSLPGADG
                     DDARARLIVELLDRHGTGRVLFRNTRAAMPNFPRRVPLPLALDCPPAYRDGDGDGVGL
                     APERAYRPGADAPAWWRFDPRVDALIELLAAGDKVLLICAHKATVLELDEALRVKSGV
                     PAALFHEDMPLLARDRAAAWFADPAGARVLLCSEIGSEGRNFQFAHHLFLFDLPENPD
                     LLEQRIGRLDRIGQTEDIRIHLPWLRATAQEILYRWYAEGLDAFARHARAAEAVYRRV
                     RPELEAALAGHGDAEALIAHSARLNAELGSELERGRDRLLELSSFREGPALELVERIR
                     AQDADPALPALLERLFDHYGVDAEAHEDGTLVLRTGEQMLTAQFPALPEEGLAVTFER
                     GLALAREDVDFLSPDHPLVVGALDLLAGAVDGNCALVAWREPPDEAPELLLEAVYVLE
                     CVAPPALQADRFLPPLPLRVVIDPHLRDAGEHWPAAVIGSGCTDVPAAAVRERLADRR
                     LRRMLDAAAVAVGRLAEQTIKAAQARMQATLTPEVERLRALAAVNPSVRAAEIEFAET
                     RVAELYTHLAAARPRFDALRVVLRPGA"
     gene            27881..28789
                     /locus_tag="PK144_01005"
     CDS             27881..28789
                     /locus_tag="PK144_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF64328.1"
                     /translation="MRLTLDALAVLDAIARRGSFAAAADELHRVPSAITYTVHKLEQD
                     LDVQVFDRSGHRARLTPVGETLLAEGRHLLRAAGELECLVRRVATGWEAELSIALSAL
                     LPLTPLLPLVEAFDRETPGTRLRLGTEVLGGVWDALVTGRADLAIGASGDGPVGGGYA
                     SRALGTIGFVFVCAPQHPLARSEVPLGEETILASRAIAVADSSRVLAPRTAALLSGQP
                     VLTVPSMEAKIDAHRAGLGVGYVPRAWVREDLAAGRLCECAVEHPLPPAPLYLAWRTG
                     NGGRGLKWFLRALEELAPWRDWLDAE"
     gene            28877..>30247
                     /locus_tag="PK144_01010"
     CDS             28877..>30247
                     /locus_tag="PK144_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF64329.1"
                     /translation="MASVQRLPAFFDHAERIRREAARYLFDYFEACCEGAMAVDREAR
                     IVWINEPYAQALGLADAAAALGKPVEDVIPHSLMREVVQSGRPILLDIMQHGARSLVV
                     TRLPLTDEHGAVIGAVGFVLYDDPRSLQPLMTKFSRLRAELDAARRELAASRRARYRL
                     DDYVGSSPAALEVKRQAARAAASDSTVLLLGETGTGKELIAQAIHAASARAARPFVGI
                     NVAAVPDGLLEAEFFGSAPGAYTGADRRGRDGKFKLADGGTLFLDEIGDMPAHLQAKL
                     LRVLQEQEFEPLGSNRVERIDVRVIAATSRDLPAQVAGGRFRADLWFRLNVLPIRLPP
                     LRERRDDLPLLAGALLDRLSARAGGVRRVLGASALAELAGYDWPGNTRELGNVLERAV
                     MLSDRRCYEAADLAGLLRPPAAAAASAPAAPAVQRLADAVAEAERTAIRSALAVSGGR
                     KVDAAKL"
BASE COUNT         4344 a        10262 c        11096 g         4545 t
ORIGIN      
        1 gcgcgagtct tgcgccgccg cgcaggtgca gcacgacggc atgatccggg gcacgcaccg
       61 cgacggcatc gagcgtgcgc agcgcctcgg ccggccggcc ggcaccgagc tgcaggctgg
      121 cgagcagcag gccgtcgcgg gcttcgccgg attgcgccca ggctgcggac agcgcggcga
      181 gtgccgcatc gccctgaccg ctctgcaggc gggcctggcc gagctgcgcg aacagggccg
      241 gctgctggcc gccgagttcg atcagccgct ccagcagcgc ggccgctgtc gcatgctgtc
      301 cgagctgggt atgggcatcg gcgagcaggg tcagcaggcg tacgtcactg acggcatgcg
      361 gatcgtccgg cagcggctgc agcagttcca gcgcctggcg gggatcgccg aggcgcagca
      421 gcaccgcggc gagcagccgg cgtgcagtct cgtcacccgg catgcgctcc agatactgcg
      481 tcaggtcgcc gcgggccttt tcggacaggc cgagcgcctc gtgcacggtg ccggacaatt
      541 tcagcagcga gggcgagcgc agcagcgtct cgcgcgggat ggccttcagc agcagcgagg
      601 catcggtcag gcgtgcgcgt gccgtttccg ggtcctggcg cgcgcgggcc agttcggcgg
      661 cgagcagcag cgtgcgcaca tcggccggat tctgccggcg caggcgatcg agcaccggct
      721 gcgcctcgtc ggcacgcccg agcgcgatca gggcgctgac gcgcgcggcg cgcgccggca
      781 ggcagagttc atccagtttc agggcggtgt cggcggcggc gagggcctcg gtggcatgcc
      841 cggcagcgag ttcggcggcg gcgagcgcgg tccaggcttc ggtcgagcgc ggatcgaggc
      901 cggtggcgcg ctgggcggcg ctgcgcgcga gcgcggcatc ctgctgctgc agggccagca
      961 tcgacaggcc gatgtcaccg tccggcgcct gcggtgcggc ctcgcgggca gcggtgaacg
     1021 acagggcagc ctcttccatc agaccgagtt cgagccgcgc ctcaccgcgc aggcgcagca
     1081 tgcgcgcgcg cgcggcgccg gtatggcggt acggatcgaa gccgtcgagg acggcctgga
     1141 actctccctg gcgcagcttc aggtacatgc gcagcggatc gatgtcggcg cgctcggctc
     1201 cgaggcgcag cgccccctgc agggtgcttt ccgcgagttc gaaccgcccc tggcgcagat
     1261 aggtgtgacc gagcgcgacc tgggccggag cgaggtcggg gcgctgctgc aaggcattct
     1321 tcagatggat ttcggccccg gcgtaatcgt tgcggtcgag gctctgcagg ccggcgtgca
     1381 gttcttcggt ggcggtgccg gcgacggccg gcggaccgcc cagggcgaag accagcgcca
     1441 gcgccagcgg ctggaggcgg gagcggttca tcggcagttt cctggcacgg atggcagcgg
     1501 acaccccctg gcccgcaagg cgggcgaggg gggcgggcag cgacggggag cgcgcaggcg
     1561 ctcagcgacg ccggcggcgc atcagccagg gcagcgtcag tccgagcagc gcgaggctgg
     1621 ccggttccgg gacggaggtc acgctgctgg agctggcgat gaagatctgc tcgtaaccgt
     1681 tgttcagggc cgacatgcgg aagtcgaact gcatcacgtc gtagagacca ttggcgatcg
     1741 cggtgttgag ttcgggcgaa tacagcatgt aggcggcctg gttggcgccg aggttgtggt
     1801 tgaaggtctg gtcggcatcg ggcacgcagc ccgggatctg gtagccgaag atgctgcggc
     1861 aggtgtcgga actcgggatc gtgacctgac cgccggagag gatgtagtag aagtcggtcg
     1921 tggtgatgct ggccggagcg gcgttgaggg ccggggtgtc accggcgccg tgcgtgtagc
     1981 ccagcgtctc ggcgccggtc agcgaaggat tgccgccggc accgcggttg ttggtcaggt
     2041 cgaagaccag atccggcgcg acgccggcat cgtccttcag cgtgaactga ccccaggcca
     2101 tcaggttctg gttggtggcc gcgccggaat tgacctggtt gttgttgaag aagaacagca
     2161 ggtcaccgcc gttcaggaag ctggtcagcg ccgagacgcg cgcatcccag gtgttttcgc
     2221 cgtcaccgac gaactcggtg gtgtgattcg ccggattgcc gttgtcaccc gggtcatcga
     2281 caccgcccgg agccgaggtc gtggtctgga actggtcaac gccggtgttg ttcggcgtct
     2341 ggtaggcatt gtcgacgccg ctgtaattcg tattgaccgg ctggccgttc gcgccggtgc
     2401 cgatgacggt cagatcggaa atctgtccgg ggccggaggc gatgtagaac ggattgccgg
     2461 ggccgacgcc acctccgaat tcgcgcgcat aacgatatgc gaggatcggc agcgagtacg
     2521 aatagaagtc gccgtactgg aaggcgttga actgcggggt cggcaggaag aaggcagcct
     2581 gggtcgtcgg aacgccgccg atcagacagg ccgctccgat tgcggctgcc agcatgcgct
     2641 ggttcatgtt gctcacctca tgtgcatggg ttttgcgcgg atttcttatg tgctgcgata
     2701 catggctttc acctccgctg tgtgaatcgg ctggccggcc ctgacctggc gcacgcgggt
     2761 tctcgtgctg cgccagtgat cccgaatgtt aaatacagca agttacgtgc cgtaagcttg
     2821 cttacatgaa aaaacaaggg gtttggtctg accggcgggc aaattaggtc gcacagctga
     2881 taaaaatcct gacagacccc ggcccacgac cacggtcggg ggcggcggca ccttgcttat
     2941 actgatcggc cccgagctgc cgatgtgccg accgccatgc cctatcgcct gctgcgcccg
     3001 ctgctgttcc ggctcgatgc cgaacgtgcc cacgacctga cactggctgc gctcgggcgc
     3061 atcgggccgg cggcgggact gatgacacgc gcggtgccgc gccggccgcg cgtggtcatg
     3121 ggcatcgagt ttccgaatcc ggtcggtctg gctgccggcc tcgacaagga cggctcggcg
     3181 atccgcgggc tggcggccct cgggttcggc tgcctggaga tcggcacggt cacgccgcgc
     3241 ccgcaggccg gcaatccgcg cccgcggctg ttccggctgc ccgaggcgca ggcgctgatc
     3301 aaccgctacg gcttcaacaa cggcggcgtc acggcgctgg tcaaccgggt catggcggca
     3361 cgcgcggccg gctttcgcgg cgtgctcggc atcaacatcg gcaagaacaa ggacacgccg
     3421 aacgagcgcg ccagcgacga ctacctgctc tgcctgcagc aggtctacgc gcacgccgat
     3481 tacgtggccg tcaacatttc ctcgccgaac acgccgggcc tgcgcgacct gcagtacggc
     3541 gaggcgctcg accggctgct cgacgaactc aaggaggcgc agacccgcct cgccgaccat
     3601 cacggccgct atgtgccgct ggcgctgaag atcgcgccgg atgtggcgga tgccgacctg
     3661 ccggtgatcg ctgcggcgct ggtgcgggca cgtctggatg cggtgatcgc gaccaacacg
     3721 accttctcgc gtgccggcgt cgagcacctg cgtcacgccg gcgagaccgg cgggctgtca
     3781 ggggcgccgc tgcgcgagcg ttcgacggcg gtgatcgccg gactggcgaa ggcgcttgat
     3841 ggtgcgctgc cggtcatcgg cgtcggcggc atcctgagcg gcgatgacgc ccgggccaag
     3901 atcgaggccg gggccagtct ggtgcagctg tactcgggct tcatctaccg cggaccggcg
     3961 ctgatccgcg aggcggtcga ggcgatcggc tgacgcccgc gcctgagcct ggggctgccc
     4021 gggctcaggg atcgtcggcg ctcggcaccc agccgccgag atccttccag cggttgacga
     4081 tgttgcagaa gagttccgcc gtgcgctcgg cgtcgtagac ggccgagtgc gcctcgcgcg
     4141 catcccaggc gatgccggcg gcctgcgcgg cacgggcgag cacggtctgg ccgtaggcga
     4201 gaccggcgag cgtcacggtg tcgaaggtcg agaacgggtg gaagggattg cgcttcacgt
     4261 cgcagcgctc ggccgccgcg ttcaggaagc cgagatcgaa cgcggggttg tgcgcgacca
     4321 tgatcgcgcg cgtgcagccg gtctcgcgca cctcgcggcg cacctcgcgc agcacgttgc
     4381 cgagtgcttc ggcctcgggc agcgccagcc ggaacgggtg gaaggggtcg atgccgttga
     4441 ccttcagcga ggccggatcg aggttggcgc ccgcaaacgg ctcgacgtga tgcgcgatcg
     4501 tgcgtgccgg atacaggcgg ccgtcgctgc cgaggcgcag cagcaccgcg gcgatttcga
     4561 gcagcgcatc ggtgcgggca ttgaagccgc cggtctcgat atcgacgacg acgggcagaa
     4621 aaccacggaa gcggaaggcg atcggggact tgcggacgga atcgttcatg gcccgagcat
     4681 acccgaggcg cctgcgcttg ggcatcgcgc ccgccgcggt gcgccgccgg tcacaaggcg
     4741 ctacagtcgc gcctgccttc ccgtcccaga ccccgcaccg aacggagtgt tgccgatgcc
     4801 gtacgcgtcc gccgaacagg atgtcctgcc gctgcgtccg gccgacggca gcggcagcgg
     4861 ggcctgagcg atgctgcgcc gccgcttttt ccgcaatccc gtcttcgcga atctggccct
     4921 gctgctcgcg ctgctcgccg gtgtgctgtc ctggtggcag atgccgcgcg agcagaccgc
     4981 acgaccgtcc gcggcggccg tcgagatcgt cacggccgca ccgggactcg cgaccgccga
     5041 gatcgagacc cgcgtcaccg cggtgctcga agccgcgatc aaaccgatcg ccgatctcga
     5101 cttcatcgcc agcaccagcc gcgtcggcga atcgcgcctg accgtgcgcc tgcgcacggt
     5161 cagcgaggcg accttcgagc gccggctgaa cgagctgcgc tggatcgtgc agcacgtcga
     5221 gcgacagctg ccgtcgctgg ccggccgtcc ggagatcgcg agcgtgcatg gcgccggcgt
     5281 gctgccgagc gccgacgtgc tggtgctggc ccgggccccc gatgcgccgg cgccgtacgc
     5341 ggcggccgag cggctggcgc agtccttcga gcggctgatg gatgtcgagc gcgtcgagcg
     5401 ctacggcacg cgcgccacgc gcgtcgagat ccgcttcgat ccggagcgtg tcgcccgtca
     5461 cggcctgacg ccggcgcggc tggccgaact ggtcggggcg cagctgcgcg agcaggcggt
     5521 cgggcgcgtc gacttcgacg agcggccgat gcagctgctg gtcgagggtg ccgacctgca
     5581 gcccgagcgc ctcgcgcagt tgccggttcc gggtgtccgc ggcctgcagc cgctcgcggc
     5641 gctggccgac atcgagcgcg gccccgaacc ggcgacgcgc gcggtgctgg ccgacggccg
     5701 gccggcggtg ctgctgtcgg tgtaccgcaa gtccgacgcc aacgcgctcg acttgtcggc
     5761 ggcactcggc gagcaggcgg cgcgcttcgc ggacagcgat gccgaggccg ccgcgatcga
     5821 gctgcgcgtg ctcgatgcgc gcagcaccga cacccgtcgc gcgctgcagg cttacgagcg
     5881 cggcgcgctg ccggcgctcg ccgccgcgct gctggtgttc gcgatcgcct tcggctggcg
     5941 cctggcgatg ctcgttgcct gcgtggtgcc ggcggccgtc ggtctggcgc tggcgccgtg
     6001 gggattctcg ggcggcgtgc tgacgccggt gatgctgacc ggcgtgctcg ccggtttcgt
     6061 gctgctgatc gatgcggcag tcgcagtcgg cgagcgcctg cagcaccggc tgctgcgcgg
     6121 tgccgcggcg ctcgatgccg cctacggtgc ctggcgcgag gccggactgc cgctgatggt
     6181 cgcgacgctg gtgttcctgc tggcgatgct gctgccgatg tggctgccgg aaggcagcgg
     6241 gccggccggc gcgctggcgg cgcgggtcat gaccggcatg ctggtcgccg gcatcgccta
     6301 cgcggtgctg ctgctgccct cgcacgcggt ggtcacgcgc atccgcctgc cgcgccgcca
     6361 tccggtgcag gcccggcgcg aatggctggc ctggcggctg cgccacgcct accggcggct
     6421 gctgaactgg acgctgcgcc ggccgctggt gccggggctg gtgctggtgc tgctgctgct
     6481 cggcgcgctg gtcgccctcg gcagcggccg cgtcggcgtc gagttcgccg aacgcgggca
     6541 gctgcgctcc ttctacgtga acctcgaact gtcgccgggt gcgaagctgg aggacaccct
     6601 cgccgccggg cgtgaggtcg agcgcatcgt gcgcgagcag ctgccggccg atgcgctggc
     6661 cgaggtcgtg atcagcgccg gcctgcgccg cagcgagcgc gaactgctga ccggcgagcg
     6721 ccacggccag gtgctggtcg gcctgcgcag cggcgaccgc atccgcgacg cgctgaccgt
     6781 cgagcaggcg atcacgcgcg tcaagccgcg gctcgccttc gtcggtggcg tcgacaacct
     6841 gagcgtgcag gccgtcgacg acggccggcc ggtggcgcgg ccgctcagcg tcaccgtgcg
     6901 cggcgatgat ccggccgagg tgcgccgcgc cgccgaccgc gtcgtcgaca tcctgcgcag
     6961 cctgatcggc gtcaccgatg tggtcgacga cgaggatcag gccgaggccg cggagctgcg
     7021 cctggtgctg aaaccggggg cgctgctgcg cgccgggctg tcggcgccgc agctgctgcg
     7081 cgagctgcgc ctgctcggcg agggcgagac catcgcgcag ctgcgcgagc agggccgcaa
     7141 cgtcgacgtg ctgctgcgcg gcaaggctgc gcccgaaccg ttcttcgaca tcgaggaatt
     7201 cctggcgcgg ccggtgcgcc tgcccgatgg ccgcagcgcc ccgctcggcg agttcgccag
     7261 cgccgagccg gccagcggcc gcagcgtcgt gcgccaccag aacctgcgcc gtgcgatcag
     7321 catcgaggcc gacgtcgatc aggcgcgcgt caacaccttc gcgctgaacc gcgtgatcgc
     7381 caccgaatgg gagcgcacgg ccgccgccgg cctgcccggc gtcgaactgg tcatcggcgg
     7441 cgccttcggc agcgtcgccg gtcacctcgg tgcgctggtc agcctgttcg cgctcggcag
     7501 cggactcggt tatctggtgc tcgcgacgcg cgcgcacagc taccgccagc cgctggtgat
     7561 cttcgcggcc gtgccgttcg cggcgctggc cttcgtcgtc gggctggccg tcagcggcca
     7621 gccggtcggt ccgtacaccg gctttgccgg cacggcgctg ctcggtctgt cggtgcagtc
     7681 gacgctgctg ctggtgtcgg ccgtgcagcg gctgctgccg atcgagggct cggtcgccag
     7741 agccgtggcg ctcgccgcgg ccgaccgggc gctgccggtg ctggtctcgg ccctggcaag
     7801 cgtcgccggc ctcgcggtat tcgcgctcgg cgtgccggtg gcggcgccgg cgtggatggc
     7861 gctcgccggg gcgctgatcg gcggcattcc ggtcatcgcg ctgtgcgtgc tgctggtgct
     7921 gccggcgatc tgcctgtacg tgctgcgcgc gccggtgtcg gcggtcacgg cgatcaacgt
     7981 cgtcgacgcc gaaccgggcg gcggcagctg gcgcgagcgc atgcgccgcg gccggcggca
     8041 tgcggcgatc gtcgcgctga acgaatccga gctgaacagc gccgagcccg agctgcgcgc
     8101 ccgccacgag gccggtctcg acgcgctgaa ggtcggcgag tggctgcagg cgctgcgcct
     8161 gttcgaggac gccgccaagc gtcaccccga ggccgtgcag ctgcagttcg gcgccacgca
     8221 ggcgctgctg cagtatctgt tcgagaacga atgggacgag ggctatcacg cccgtgccgg
     8281 ccggcatctg gcgcgcgcgc gccagctcgc ttccggcgat ccgcgcctgc gtccgctgga
     8341 acgggcgctg gccgaactcg aagcccggca tgtgccggtg gccggggagg gcacaagcag
     8401 ccagcccagg gtcggcatgc tgcggctgtc gggcacggtc ggccggccgt ggcggccggc
     8461 gagcgtggcg cgcgggcagc gggcgcccga tcgcggcacc gatctgccgg ccggcctgca
     8521 tgatctggcg ctggcgctgc gcaacctcgt gcgccagcgc aagcgcacgg ccgccgcgct
     8581 cggcgcgatc ggcttcggcg ccgtggcgct gatcctcgcc ggcggcttca tcgaatgggt
     8641 gctgtgggcg acgcgcgaat cggccgtcta cggccagctc ggccatgtgc aggtcgtgcg
     8701 ccaggatttc ttcgtgcgcg gcacggccga tccgcgcggc ttcctgatcc cgggcgatgc
     8761 gccgccggcc ggcctgcgcg agctgccgca cgtgcgcgcc gtgacgccgc ggctgagctt
     8821 cagcggtctg gcgagcaccg gcgagaccac ggtgccgtac ctcggtcagg gcgtggtgcc
     8881 cgaggacgag gcgccgatca gcatcgatta ccgcatcgtc agcggcaagg gcctgaccac
     8941 cagcgacgcg cgcgaggtca tcgtcggcac cgggctcgcg gcgacgctcg gcgtcaagcc
     9001 cggccagaag ctgatcctga tgagcaactc cggcagcggc gcgttcacgc cggtcgagct
     9061 gaaggtgcgc gggctgttct cgacctcgaa caaggcgctg aacgatgtcg ccctgcgcgt
     9121 gccgctgaag accgcacagg acctgctgca ggtcgatggc gtgcacatct gggtggtgct
     9181 gctcgatcgc accgaatcga cggccgccgt gctgccgcag ctgcgcgccc tgctgccggc
     9241 tggcctgcag gcggtgccgt ggtcggacct cgccgacttc tacaacaaga ccagcgagct
     9301 gttccagcgc cagatgttcg tgctgcggct gatgatcgcg gtgatcatcc tgctcagcat
     9361 ctcgaactcg ctgatgatga acgtcatgga gcgcaccggc gagatcggca cgctgtgcgc
     9421 gctcggcacg cgccggcgct cgatcctgaa gatcttcagc ctcgaagccc tcgcgctcgg
     9481 cctgctcggc gcggcgaccg gtacggcgct cggcgtgacc atcgcctcgg tggcctcggc
     9541 ggtcggcatc ccgatgccgc cggccccggg catggacaag ggcttcgtcg ccgagatcct
     9601 ggtcacgccg gcgctggcgg gctcgacctt cgggctggcc gtcatcgcga ccttcgtctc
     9661 cggcctgtat ccggcctgga aggcctcgcg gctcgtcatc gtcgacgcgc tgcggcacaa
     9721 ccgctgaagc ggagcgcgcc atgctcaagt tcgcctaccg caacctgacc cgccaccgcg
     9781 gccgcacgct gctgacgctc ggggcgatcg ccttcggggt cgtgagcctg gtgctgtcgg
     9841 ccggcttcgt cgacgacatc ttcgtgcgcc tgcgcgagaa caccgtgcag agcggcctcg
     9901 gccacctgca ggtcgcgcgc gagcactact tcgacctgcg caaccgccag ccctatgcct
     9961 acatgatcga aaaccccgga gccgtcgtcg ccgccgcgaa gaagctgccg acggtgcgcg
    10021 aggcgatgat gcgcgtgaac ctcaccggca cgctcagcaa cggcagcggc gagctgccgg
    10081 tggtcgccga gggcggcgag ccggaaaagg aggtggcggt cggcggcttc gtgacgctgg
    10141 tcgacggccg gcgcctcacc ggcggcgatc agaacgtcgt cgagctcggc gaggggctgg
    10201 cgcgggcgct cggcgttgcg ccgggggcgt tcgtgacgct gctgaccgcc acgcccgagg
    10261 gcgcgatcaa caccgtcgag ctgaaggtgg tcggcatctt ccgcagcgtg tcgatcgact
    10321 acgacgcgcg tgccgcacgc ctgccgctcg cgacggcgca gacgctgctg aacacgaagt
    10381 ccgtgcatac cgtcgtcgtc gcgctcgaga acaccgcctt cacgcagacc gctgccgggc
    10441 agctgcgcaa ggccctcgat ggccagccgc tcgccgtgta cacctgggac gaactcgccg
    10501 atttctaccg caaggcggtg gatctgtacc gcacgcagtt cggcgtgctg caggcgatca
    10561 tcctgatcat ggtggcgctg tcggtgtcga acagcatcaa catctcgatc ttcgagcgca
    10621 cggccgaggt cggcacgctg ctcgcgctcg gcaaccgccg ccggcaggtg ctgcagctgc
    10681 tggtgatgga gagcgcgatc tcggggctgg tcggggggct ggccggcgcg gcgctcggca
    10741 tcgggctggc gctcggcatc agctcgatcg gcattccgat gccaccgcca ccgaactcca
    10801 gccagggcta tctcgcggtc gtgcgcatcg tgccggagca ggtggcggtg gcgctggtca
    10861 tcggcgtcgt tgcgaccgtg ctggcctcgg tgctgccggc gtggcgggtg tcgcggctgc
    10921 cggtcgtcga agcgctgcgc cgggcgcaat gagcccggtg cggccgcagg gtccggaccc
    10981 gcgggcttca gtcgatgccc tgcgtgacgc gcttcatgta gtccttgttg aacagcttgt
    11041 tcggcagctc gcgcggcttc atcgcggagt aatcgagcac ggagcggtca ccgcgcttga
    11101 ccgcatcctc catgaccagc tgggtcggac gcacggtgtt gtcgagcttt gcgtagttca
    11161 ggtactgggc ggtcttcagc agccggccgg acagcgcata gaactcggcc ttcagcggct
    11221 ggttgccgct gcgctcgacc cacagcagca cgcggttgta ggtgacgctg ccgtcgatgg
    11281 cggtcagctc cagcactgcg tacttgcgct tctggatggt ctcgtcgcgc agcacgcggg
    11341 cgttgtagtc gcccgagaag ttcgcgcgtg ccagatcgcc gttggcgacc tggccggtca
    11401 ggcgctgcga cagcggcagg cgcaccggct gcgacaggcg cggcgagtac acccacaggt
    11461 cgcgcccgcg catcagcagc acctggccgc gctccgaagc cggggcggta gtgatgacga
    11521 gggtgttttc gttacccttc gacaggatct ggtaatcgcg gacctcggtt tcgcctccgg
    11581 gcgtggtcga gctgatccgc acgttcacct cgaagtcgct gcgcggaaac cggacctgat
    11641 cggcggcctc cagcatcgcg agggcttcga ggtccacttc ctgtgcccgc gtcgccggcg
    11701 tcgccagggt ggcggcgcac agcagcagca gggaaggcag acgcagtacg gtcgcacgca
    11761 tcggcatact ctccaggcat cgggtacaca acgggtacgc ccggtcgcag ggccgggcag
    11821 ggacacggcg cgagccgcgg gatgaagaac gggtcatgag catagtacgc atcgaaaacg
    11881 tctcgaaacg ctaccgcctc ggcgagcagg acgtgacggc gctgcacggc gtcagcctgc
    11941 gcgtcgagcc gggcgagttc gtggcgctgg ccggaccctc cggcagcggc aagacgacgc
    12001 tgctgaacat gatcggctgc atcgacacgc cgagttccgg gcgcgtcatc gtcgatggcc
    12061 acgacgtgac cggccgcgat ccggaccagc tcgccgaact gcgcgcccgc acgctcggct
    12121 tcatcttcca gaccttcaac ctgctgccgg tgctgtcggc cgaggaaaac gtcgagtacc
    12181 cgctgatcaa catccagcac atcccccggg ccgagcgccg ccagcgcgtc gcgtacttcc
    12241 tcgacatggt cggactcgcg cggttcgcac gccatcgccc gaaccagctt tcgggcggcc
    12301 agcgccagcg cgtcgcgatc gcccgggcgc tggcgaccaa gccgaagatc gtgcttgccg
    12361 acgagccgac cgcgaacctc gatcaccaga gcggcgacgg tatcctgcag ctgatgaagc
    12421 acatcaacga gcagtccggc accaccttca tcttttcgac gcacgacgcc cgcgtgatgg
    12481 cgatggccag ccggctcgtg cgcatcagtg acggcgccat cgtcggcgag gagacccgat
    12541 gacgaaccat ccgaaccggt ccggccgcat cgccctgggc atcgcactgc tggcggtcac
    12601 gccggcggct gccgcgcagc aggacgtgaa gctcagcggc tacctgtcga acgagaccgc
    12661 gtacaccttc ggcggcgaga atcactggtc gaagtcccgc aacacgctga acctggccgc
    12721 caccggcagc ttcagcgagt cgatctcgtg gaaggcgagc ggctggctgc gctgggaccc
    12781 ggttgtccag cactcggatt tctatccgga caaggtgcag gacgaccgcg gtttcgaggg
    12841 gctggtgcgc gagaccttcc tcgatgtgag cctcggggac tgggacctgc gcctcgggcg
    12901 ccagaacatc gtctggggcg agatggtcgg cctgttcttc gccgacgtgg tctcggcgcg
    12961 cgacatgcgc gagttcgtgc tcgccgactt cgagcagctg cgcatcccgc agtgggcggc
    13021 gcgcgccgag tacttcaagg acgacttcca cgccgagctc gtgtggatcc cgtacctgac
    13081 ctacgacaac atcggcgagc ccggcgacga cttctacccg tatccgccgg ccttcccggg
    13141 gctgggctac cgcatcctcg atgaaaagaa accgcgcggc ggtgacagcg acgatgccgc
    13201 cggcgtgcgc ctcggctgga tcgtcgatgg ctgggacatc aacggttact actaccgcag
    13261 caccgacgtg cagccggcct tccagcgcag cttcggcctg gtcggcggcg tgccgacgct
    13321 gacctacacg ccgacgcaca accgcatcca gcagtggggc ctgaccgtcg gcaaggacat
    13381 cggcgacggg ctggtgttca aggccgaggc gatctacacg atgggccggc gctacgacgt
    13441 gctgcgcctg agcgatgccg acggtctggt gcgcaaggac acgctcgatt acgtgctcgg
    13501 tctcgactgg acgccggacg ccagcaaccg cctgaacctg cagttcttcc agcgcttcta
    13561 tcaggaccac gacccggaca tggtgccgga gagcgtcgag agcggtgtca gcctgttcta
    13621 ttcgaccaag gtctggggcg accggctgga gccggaaatc accttgatcg agagcctcaa
    13681 ccgcggcgac tggctgctgc ggccgcggct gacctggaac gtcagcgccg acctgaaggc
    13741 cagcttcggc gccgacatcc tgaacggccc gccggatgcg ctgttcggcc gcttcaccaa
    13801 cggcgaccgc gtctacgccg aggtccgcta cgacttctga gggctggcgt ccgggctgcc
    13861 gccgccggtc atcagccgcg cgagcggcag gcgcgtcgag cgcgataccg gcaccgggcc
    13921 gtagccgagg cggccgaagt acacggcgcg cgccgcggtg tcggccccga ccagtgcatc
    13981 gagctggcgg gcgagcgtgc gggcgttgtc gaccgaagcc tgctttgtcg tgaattcgat
    14041 gccgtcgcgg atgtagccgg agaagatcag cggcgtcatc tccggctgga actgcaggcc
    14101 gaggcgggtc gcggtcagcc agaagcgctg cattgcggcg ccggcggcga gatagtcggc
    14161 gagcgtctgc ggtgcctgtt cggcaatgat caggaaatgc gcggcgcagc cgagcgccgg
    14221 tgcgaagtcg agctgcagcc gcggcagcca ggtgccggcg aaccagcggt tcaggaagtc
    14281 gacgcgctcc cagctcttca tcgcccagcg catcagcgcg agcccgaccg gatcgagccc
    14341 gaccgcggcc ccgggcagcc ggtcctcgct gtactgcacg ccgaactcga tgatgtcgcg
    14401 gtgcgtcggg taggcctcgg gcgtcgtcag ccggatgtgg gcgttgcgaa acagcagctt
    14461 cgcgacatcg aggcgctgat ccccttccag ccagcgcagc gagtagccgg gaccgagcgc
    14521 ctgcaggaag gccgccttca cggcgtccgt gagcggccgg cgcgacagcg gccggcgctg
    14581 cgtgcagcgt gcgcggatcg cggtttccag cggatggcgc acggccgccg ctgccggcgt
    14641 gaactgcagg cggaacatcg gcgcaaacgc cggcgtcgtg tccccgacgc cgggcagcgt
    14701 ttcgatgtcg agcgcaagcc ccgcgcccgg ggcggcgatg cgcgcggttt ccagcagcgc
    14761 gccgagggcg agctggctcg gccggccgcg caggtcgtag atcacgtgat cgcgcgtgtc
    14821 gcagccgtgc acgatcacgg tcagcgggtc gacggcctcg aagcgccacg gctgggtgtt
    14881 gtcgccgctc ggggcccagc tcgccagttc gagcacgcgt gcgacgcgcg ggtccagccc
    14941 ggccggtgcc gcaggcgcgg cacgcgccgg ggccggcagg ctgcgggcgc ggcgcagcag
    15001 cgtgcgcatg atcgccagcg tcaggcggtt cagcgggtgg cggttgccgc ccgggcgcca
    15061 ggtgcgcgac agccggttgc ggtaggcatc gaagtgcagg ccccacggcg cggcgcgcac
    15121 cgggccgcgg ccgagcagca gcttcagcga ttcggtcacg agcacgccgg cggcgagttc
    15181 gcaggccatc ggcgtcgacg gtccgcgctt ctcgacgaag ttgatcgtcg agcgatcgac
    15241 cagatagccc tgctgcagca tcgtcggcga cagcccgacc aggaagcgcg cgtactgttc
    15301 ctcctcgggt tggccctcga cctggaagta gtcctcgaag ctcatgcgcc cgggcaggaa
    15361 ggtcagcacc gccgtgccca tgccgagcgg cgcggccgtg atcgccggga tgccgcgggc
    15421 gtgggcggcc gcgaacacct tgcgccgcac cggcagcacg aagaaatcca gcgcatcgac
    15481 gtacaggtcg acgccgtcga agaaggcatc gaggttgtcg tcggtgacgc cgtccgcaaa
    15541 gcgccggata tcgagttccg gattgatcgc gagggcggcc tcggccatca cgtcgagctt
    15601 cggccgatcg agcgtcgcca tcgtcgcgcc ggcctgacgg ttgaagttgt gcagctcgaa
    15661 gcggtcgaag tcggcgaggt ggaaggcgcc gacgccgagc cgcgccagcg tcagcagatg
    15721 cacgccgccg acgccgccga gtccggcgac agccagccgc cggccgcgca gccaggcctg
    15781 ctcggtcggt gtcagccagc cgatgttgcg gctgaaggca gtggcgtaat cgaaggactg
    15841 cgagacgtgc tgcggggaca tcggctctcc ttgcggcggc acgggggtgg ccgccggcgg
    15901 tgtccgggcg gggggaaggc cctgcaggct cagccggtcg tgttcttcgc gacccagcgg
    15961 ctgccgtcgg gcagggtttc gtgcttccag aacggcgcgc gcgacttcag cgcctccatg
    16021 atccagcgac aggcgtcgaa ggccgcgccg cggtgggcgc tccagaccgc gaccagcacg
    16081 atcggatcat tcggcagcag ctcgccgacg cggtggatca ccagcgcatc gagcagcgac
    16141 cagcgctcga ccgcctcttc ggccagacgc gcgagttccc gctcggtcat gcccggatag
    16201 tgctccagac gcatgccgcg cacgtcatcg ccttcgttgt gatcgcgcat cgtgccgatg
    16261 aagctcgccg tcgcgccgta gcgaccggcg agccgcgcat cgagcgcctg ctggtgggcg
    16321 gcgagttcgg ccagcggttc gaagccgtgt tcgcggatct cgatgcgcac ggccatgctc
    16381 agcctccggt caccggcgga aagaacgcca gttcgtcgcc gccgcgcacc ggcgcgccga
    16441 ggtcgacgta ctcgtggttg atcgccgcca gcgtgttcgg cggcagcggc agcgccgggt
    16501 tcaggcgcga ccaggcttcg gcgacggtca gcggatcgtc cccgggcagc tcggcactgg
    16561 cacgtccgac ccgctcgcgc aggctggcga aatacaggat gcggatcgca ggcatcggca
    16621 gcacctcgac ggggctcagg gctgactgtc cggggcggcg ctgagcttca ggtacatcag
    16681 cgccgtcagc accgcgtgcg caagcggcga gccgctttcg agcgccggca gttcgaggtc
    16741 gcgacggagg gtctcgaagc gcaggtccag cggccggtgc ggtgctcgcg gctggcggcg
    16801 gatgcgcgca tggtacagcg ccgagacctc gacggtcgca ttcggcagcg ggcagccgag
    16861 caggcgctcg atctgcggct ccagcagttc gaggtcgaaa tcgagccagt agccgaccac
    16921 cggccgcggg ccgatgaagc gcagcagctt ctgcacggct tcgagcgcac tcatgccctg
    16981 gccgtcgccg tgctcggggc cggcgatatc gagttgcagc gccgaggagg tcagcacgcg
    17041 gttgccgcgg atgcgcacgg ccgcgagcga cagcagcctt gcactgcgca ggtcgagcga
    17101 ggtcgccgcg cagtcgaggc agaccagctc gccggcggtc ccgccggctt cctgcatgaa
    17161 atcgaacgcc tcgggcggcg cggcgtgacg gaacggacgg gcgagggcgg cgagcatcag
    17221 gtgaccagat tcagcttgaa gtggaaggtg atgaatttct tgaattcgtt gacgatcttg
    17281 aacgaatcgc gcagcagctc gcgttcgagc ttgttcagct cgttcgggtt cacgaagttg
    17341 tcctgcggca ggccggcctt cagcttgcgc aggcccgtgg tcaggcgcag cgtcgacatc
    17401 atcgtcagcg cctcggtcag ctcggccgcc cgcgccggtg tcagcacgcc cttggcttcc
    17461 agcgcctcga tgcgctccag cgtgttggtc ggctccaggc ggtgttcgag cgcgaggctg
    17521 cgcacgccgt gcgtcagcgg gaagatgccg cccttcttga ggtcgagctc gtcgcggtgc
    17581 tggccgtcct tgccggtcac gaagttctgg aagaagccga gcggggtctc gaagctgacg
    17641 gtcgcgcgcg cgaagtgcgc gaagaagccg gagttgctct gcagttgctg gtgcaggtag
    17701 cgcttggcct cgtcgagcag ggcggtgtcg ccggcgaccg gcatcgcgtc gaagaagatc
    17761 gcgaagtgca tgaagccgtc ttcgctcggg cgcttgatcc agttgaagat ctcgtccttg
    17821 aacgcgccga gcggcttcgt ccagtacggg ttcgagacca tgatgttgcc ggggcagggc
    17881 ggatagccga aggccacgag ggcctcggtg aaggcctgcg tgatcgcatc gagcccggga
    17941 tgcgtgtagc cgtcgcgcag cagcagcgcg ttgtcctgat cggtcttgag gatctgctcc
    18001 tcgcggccct cgctgcccat gaccaccaga caggcgttgt cgatcagctc gggcggcgcg
    18061 atcagcgtga acagcttgcc gagcagcttc ttgttcagct ccgacagcag ctggccgatg
    18121 tagcgcagct tcacgccctt gccgtgcagc gcctcgatca cgcgcgtcag ctcgtggctg
    18181 gccttggcca gttcctcgcg gctgccggcg cgctcgatct gcacggcgat caggtgcgag
    18241 tggttcgaga gataagacag caggtcgacc tgttcgagca cgccgcggat cgcgccgtcg
    18301 tggcggatca cgacgcggct gatgctgtgg cgggtcatgg tcagcagcgc gttgaacagg
    18361 aagtcgtctt cttccagcga caccagaccg taggaggcga tgtcgccgac cggcgcatcc
    18421 ggcgagcggc gttcgatgac gacggcgttg cgcaggtccg tgcccgagca gatgccgatc
    18481 tcgccgtcct cgcgcacgac cagcagcgag gtcgcgcggt tgtcgcgcat cgcgcgcgca
    18541 gcctcgtgga tcgagcttgc ggccgtcacc tgcagcggcg ggtggacgta ggcatcgccg
    18601 atcttggcga tcatgaagcc ggccagctcg ccgtgcgtgt cgaccgggcg gctgccggcg
    18661 gtgcgtccgg cgagttccgc gaacagcgga tcggcgaatt ccgggcattc ggcgagcagg
    18721 tacaggaatt cggcgcgcgg cagcgccagg cagaccgtgt cctcgcgcgc gacgatgcgc
    18781 ccggggctgc gccggcgcag cagcgcttcg agatcgaagc agtcctcggg gccgtagcgc
    18841 gcgatgacct cgccaccggc gagcacctgc accgagccct gggtcagcac atacagcgaa
    18901 tcgggcgctt cctgctcggt cagcaccgtc tggtcgcggc ggaagaacag cacatcgacc
    18961 gcttcggcga cgcgtgcgag cgtctcggcg ggcagacgat cgaagggcgg gatgcggctc
    19021 agctgcggac gcggttccat gacatttccg taatgaaaaa ccccgccagt ctaaaagagc
    19081 cggcggggtc ctggggagcg ttccggagcc tttcggcccc ggatggcttg acttggatca
    19141 ggctcagtgg ccggaagcgc cttcggcacc gatgccggtc tggcagcgca cgtcctgggc
    19201 ctcgaagccg agcaggtcct gttcggcgcg cttgctcttg tcggtgaccg agaacagcca
    19261 gatcgcgatg aacgccgacg ggatcgagat gatcgccggg ttgttcagcg tgatcagcgg
    19321 tgcggcattg ccgatgacat cgacccagat ggccttggag atgacggtca gcaccaccgc
    19381 gacggtcagg ccgatccagc cgccgatcat cgcgccgcgc gtcgtcatct tcgaccagaa
    19441 caccgacatg atcagcaccg ggaagttggc gctggccgcg atcgcgaagg ccagaccgac
    19501 catgaacgcg acgttctgct tctcgaagat gatgccgagg acgacggcga tgacgccgag
    19561 cgtgatggtc gcgccacgcg agaccttcat ttcggtgact tcgtcggagc ggcccttggc
    19621 gatgacgctc gcgtacaggt cgtgcgagat cgccgaggca ccggccagcg tcaggcccga
    19681 gaccaccgcg aggatggtcg cgaacgcgac ggccgagatg aagccgagga agatgttgcc
    19741 gccgacggcg ttggcgaggt ggatcgcgac catgttggtg ccgccgttga ggccgctgat
    19801 cagcttggcc ttgtcgacgg cgccgttgac cagcgcgtca gccttgtaga actcggggtg
    19861 ctggacgagc agcacgatcg ccgagaagcc gatgatgaag gtcaggatgt agaagtagcc
    19921 gatgaagccg gtcgcgtaga acaccgactt gcgggcttcc ttggcgttcg gcaccgtgaa
    19981 gaagcgcatc aggatgtgcg gcaggccggc ggtgccgaac atcagcgcga gacccagcga
    20041 gatcgcgttg atcggatcgg tgaccagacc gcccgggccc atgatcttgt cgcccttcgg
    20101 gtgaccggcg accgcctgct tgaacatttc ttccggcgag aagccgaagt gcgacagggc
    20161 tgccagcgcc atgaacgtgg caccggagag cagcagcacg gccttgatga tctgcaccca
    20221 ggtggttgcg gtcatgccgc cgaacaccac gtagatcagc atcagcacgc cgacgccgac
    20281 gacggcgagg ttgtaatcga gaccgaacag gatctggatc agcttgccgg caccgaccat
    20341 ctgcgcgatc aggtagaacg cgacgacgac cagcgacgag aaagcggcca tcgtgcggat
    20401 ttcggtctgg ccgaaacggt acgaggccac gtccgcgaac gtgaacttgc cgagattgcg
    20461 cagctgttcg gccatcacga acatgatgat cggccagccg acgaggaagc cgatcgaata
    20521 gatcaggccg tcatagccgg acgagaacac cagacccgaa ataccgagga acgaggccgc
    20581 cgacatgtaa tcgccggcaa tcgcgaggcc gttctggaaa ccggtgatgc cgccgccgcc
    20641 ggtgtagaag tccttggcgg tcttggtgcg cgaggccgcc cagtaggtga tgcccatcgt
    20701 cgcggccacg aaggccagga acatcacgat ggcggtgacg ttgatcgcct gtttctcgac
    20761 cgggcccttg acggcatcgg caaaggcggg cagtgcgagg aacagcgagg cgaacaggct
    20821 ggcagggagg agcttcttca tcacttgacc gcctcgacga tttcgtgggt cagacggtcg
    20881 aactcggagt tcgcgcgcgc gacgtagata ccggtcagca ggaacgccga gacgatgatc
    20941 gcaacgccga gcggcatgcc gatgctcatc gtggcaccgg caccgacttt cgcagcgagc
    21001 gaggtcggtg aataggcgat cacgagaatg aagccgtagt agatgaccag catcacgacc
    21061 gcaagcgtcc agccaaatgc ggagcgcttg gcgacgagat cgtgatattt cggattgctc
    21121 ttaacctgtc ggacgacatc gggttgcatg gcgcattcct tatttatggc tctgattcag
    21181 aaagtcgctc tccggcccgt ccgcaccgtt tcgcatccga cctcctggat gcggcggcac
    21241 tcatcttcct gattttcgtt gcagatcagg atagtgagat gacggtctgg gccgccgcgg
    21301 agtttgaccg aacgcagggg gggaagtaaa ggccggtgac gtttacgtca acttgcggtc
    21361 gggccgccta accggcctcg gccggcagcg gcagcgcggc gaattcgagc acgcgctgca
    21421 gttcggtctc cagcagctgg aaacaggccg gtgcatgcgc ggcgtcggcg gcttcgaggc
    21481 tgcgcaggcg cgagagcgcg gcctgcgccc cgaccgagcc gagcggtccg gccagcgtgc
    21541 gcacctcgcg ccggatcagc accagatcct cgctgtcgac ggcgcggcgc agcgtcgcca
    21601 gatgctcggg aatctggcgg cgcagcaggt cgagtagccg cccggcgagg gcggcattgc
    21661 cgtggcactg cgcgagcagc gctgcgggat cgaagggggc gagccgcccg tcgatcgcct
    21721 cgcggtccag gcgcggggcc ggcgtgaccg ttgcgtcgtc gccgaagcgc accagcggcg
    21781 tgcgctcggc cgccgggctg agcgcccggg tgagggcttc ggcgaggcgc gcggggtcga
    21841 ccggcttcag cagcgacgca tcggctccgg ccggcaggct ggcggagggc ggtgtcagcg
    21901 gcgagcgcag cagcagcacg cgcggccggc gttcggcggg cgcgatccgg cgcagcagtt
    21961 cgacggtgcc gggctgggtt tcgtcgaaca gtgcgagttc gaaatgctgg ccgaagccgg
    22021 cgcgctcctg cagcagccgc tcggcctcgt cggcaacgcc ggctgctgcc acctggaagc
    22081 cccagtagcg cagctggcgt tcgagcacgg cgcgtgcgct ctggctcgcg gccagcacca
    22141 gcgcgcgcat gccggtccat tcggggccgc ggcgcacgtc gggccgggcg ggctggcgtt
    22201 cgagccgcag cgtgaactgg aagtcgctgc cgcgctcggg gctgctctcg acccagagcc
    22261 ggccgcccat catctcggcg agccgctgcg agatgctcag gccgagcccg gtgccgccga
    22321 agcggcgcgt gctggaggtg tcgccctgca cgaagggctg gaagatgcgc gcgatcttgt
    22381 ccggcgcgat gccgatgccg gtgtcccgca ccgagcagtg cagcacgacc gactgttcgg
    22441 agacttcctg cgcgcgcacg cgcacgacga tctcgccctg ttcggtgaac ttgaccgcat
    22501 tcgacagcag gtgcgagacg atctgtgcca gccgttcgcc gtcgccggtg acggcatccg
    22561 gcacgtcggc atcgacctcc cacagcagtt cgaggccgcg cccggcggcg gcgcgcgcgt
    22621 ggttggtgat ggtcgtgcgc agcgtgtcgc gcagcaggaa gggttcggtg cgcgtgacga
    22681 ggcgcccggc ttcgagcttc gagaagtcca gcaggtcgtc gatgatcgcg agcaggttgc
    22741 cggccgctcc gcgggcggtg accagataat cgcgcacctc ggcttcggcg acggcatcga
    22801 gggccagatc ggtcatgccg atcacggcgt tgagcggcgt gcgcagctcg tggctcatgg
    22861 tcgcgaggaa ttcgctcttg gcgcggctgg cggcctcggc ggcctcgacc gcccggtgca
    22921 gttcggcctc ggcggccttg cgttcgccga tgtcggagat cgtgccggtg gcgccgagcg
    22981 gccggccgtc ggggccgtgc gagagctccc agctcatcag cacccagcgc ggctcgccgt
    23041 ggcgtccgag caggcgcagc tcgctgcggc cctgcggctg atcgggggtc gccagcgcga
    23101 actgcgcgcg gaagctgcgc cggtcctcgg catcgaggca ttcgctgacg gtgcgcccga
    23161 ggctctcgtc gaggttgcgg ccggtgatct cggtccaggc gcggttcagg aaggcccagc
    23221 gaccttcggc atcggtctgg aagatgacct cgtgcacgct gtcgaccacg cgccggtagc
    23281 gggcctcgct gtcgcgcagc gcgcgttcgg cggcgcgccg ttcactgatg tcgcgcaggt
    23341 aggcggtgaa gatcgtgccg gcttcgaggt ggatcggcgt cacggcgatc tcgaccggaa
    23401 tctcgctgcc gtcggcgcgc aggccgtcga cctcgaagcg gcgcccgagc atgtcgctga
    23461 cgccggtgcc gagatagcgc tccagcccct ggcggtgacg ctcgcgcagc cgcggcggca
    23521 cgatcagctc ggccagatcg cgcccgagca cgctgccgcg gcggaaaccg aacaggcgtt
    23581 cggcggccgg gttgaactcc agcacgcggc cgcggcggtc gatcgtgatc acggcatcga
    23641 gcgcggcctg caggatcgcg ctcttgcgcg cctcgctctc gccgagtgcc tgttcggcga
    23701 gccgccgctc gctgatgtcg cgcacgatcg cgatgtacat cgcctgcggg ctgccttcgg
    23761 catgcgccga gccgaccgag acctcgatcg ggatcagcgc gccatcgcgc cggcggccct
    23821 gcagttcgcg gtggcggaag gtctcgccgg cgcgcacttc gggcagcagc aggccgatgt
    23881 gacagccgat cgcctggcgt gccgggtagc cgaacagatg ctcggccgtg gtgttgaagc
    23941 tctcgatgcg gccttcggcg tcgagcgtca tgatgccgtc gaccgcctgg cgcagcagcg
    24001 tcgccagccg ctcgccggag gcggccagtg ccgcctcgct ggcggcgagc cgcgccgaaa
    24061 cctggttcag cacctcgccg agttcgctga cctcgatcga gccgcagcgc aggtcgagct
    24121 gctcaccgcg gcgcgagtcg agttcgcggg cgaagtcggt ggcttcgttc agcgcgctga
    24181 gcggccggcg cagcgcgacc gcgagcagca gcgccccgag cgcggcggcg aacagtccga
    24241 gcggcagtgc ccgcgtcagc agccagttgc cgatcgccgc ggccggcggc atgtggactt
    24301 cgagccgcac ccagccgagc ggacgtgcgg cgttgccgac cggtgcccag gcggcggcgc
    24361 ggcgctggcc gtcggcggtg tcggccagcc agtcgaaatg cggcaggccg tccccgggcg
    24421 gcaggatcgc gctgccgctc gggcgcaggc gcgggccttc gggggtggcg tcggcgcggg
    24481 cgagcagcgt gccggcggtg tcgaggatct gcacgctgtc gacatcgccg tcgcgcgcgg
    24541 tgtcgagcgc cagccgttcg agcgtctcgc ggctcgaacg cagcagcgcc agggccgcct
    24601 ggctggcggt ctggcgtgcg agcgtggcgt tctggcgggc gacggtttcc tcgtagaaca
    24661 ccgcctgttc gcgggcaccg agcagcgcat agacggtcag cgtcgcgacc agcacggcgg
    24721 cggcgatcag cggcaggcgc agcgccagac ggcgcaggcg cggaaagagc gggctcagca
    24781 tgttcgtccg tgtccccggg cagtcgggcg cggctgcgtc cggccccgtg gccggcaccg
    24841 cgcgcccggc ggtcgatgca tcttagcgtc gccgcccgcg gcctgcccgc gggcggaccg
    24901 gcacttcagg cgcccgggcg cagcacgacg cgcagtgcgt cgaagcgcgg gcgggcggcg
    24961 gcgaggtgcg tgtacagctc ggcgacgcgc gtttcggcga attcgatctc ggcggcgcgc
    25021 acgctcgggt tgacggcggc gagcgcgcgc aggcgctcga cctcgggcgt cagcgtcgcc
    25081 tgcatgcggg cctgcgcggc cttgatcgtc tgctcggcga gccggccgac cgcgacggcg
    25141 gcggcatcga gcatgcgccg cagccgccgg tcggcgaggc gctcgcgcac ggccgcggcc
    25201 ggcacatcgg tacagccgct gccgatgacg gcggccggcc agtgctcgcc cgcgtcgcgc
    25261 aggtgcggat cgatgaccac gcgcagcggc agcggcggca ggaagcggtc ggcctgcagc
    25321 gccggcggcg ccacgcattc gagcacgtac acggcttcga gcagcagctc cggggcttcg
    25381 tccggcggct cgcgccaggc gaccagcgcg cagttgccgt cgacggcgcc ggcgagcagg
    25441 tcgagcgcgc cgacgaccag cggatgatcc gggctcagga agtcgacatc ctcgcgcgcg
    25501 agcgccagcc cccgctcgaa ggtgaccgcc aggccttcct cgggcagggc cgggaactgc
    25561 gcggtcagca tctgctcgcc ggtgcgcagc accagcgtgc cgtcctcgtg cgcctcggca
    25621 tcgacgccgt agtgatcgaa cagccgttcg agcagcgccg gcagcgccgg gtcggcatcc
    25681 tgggcgcgga tgcgttcgac cagttcgagt gccgggccct cgcgaaacga cgacagctcc
    25741 agcagccggt cgcgaccgcg ttcgagttcg ctgccgagtt cggcattgag ccgggcgctg
    25801 tgcgcgatca gcgcttcggc atcgccgtgg ccggcgagcg cggcctcgag ttcggggcgc
    25861 acgcgccggt acacggcctc ggcggcgcgg gcgtggcggg cgaaggcatc gaggccttcg
    25921 gcgtaccagc ggtacaggat ttcctgcgcc gtggcgcgca gccagggcag gtggatgcgg
    25981 atgtcctcgg tctggccgat gcggtcgagc cggccgatgc gctgttcgag cagatcgggg
    26041 ttctcgggca ggtcgaacag gaacaggtgg tgcgcgaact ggaagttgcg gccttcgctg
    26101 ccgatctccg agcagagcag cacgcgcgca ccggccggat cggcgaacca ggcggcggcg
    26161 cgatcgcgcg cgagcagcgg catgtcctcg tgaaacagcg ccgccggcac gccgctcttc
    26221 acgcgcagcg cctcgtcgag ttcgagcacg gtcgccttgt gcgcgcagat cagcagcacc
    26281 ttgtcgccgg ctgcgagcag ctcgatcagc gcatcgacgc gcggatcgaa acgccaccag
    26341 gccggcgcat cggccccggg tcggtacgcc cgctcggggg caagcccgac tccgtcaccg
    26401 tcaccgtcgc ggtaggcggg cgggcagtcg agcgcgagcg gcagcggcac gcggcgcggg
    26461 aagttcggca tcgccgcgcg cgtgttgcga aacagcacgc ggccggtgcc gtggcgatcg
    26521 agcagctcga cgatcagccg cgcgcgtgcg tcgtccccgt cggctccggg cagcgacagg
    26581 cgttcgagca tcaggtcgcc ggtctcgggc ccgagacagg cgccgagttc atcgagatcg
    26641 gcctggtcga gcggccggta ggcgagcagg tcatcgacga tgcgcgcgac cttgcggtag
    26701 gcgagtgcct cgtgccggaa gcggtcgagg tccgggaagc gctcggggtc gagcaggcgc
    26761 aggcgcgcgt agtggccgtt cggaccgagc tgctcggggg tggcggtcag cagcagcagg
    26821 ccttgtgcat gtgcggcgag gcgttcgatc gccgtgtact cggggctcgg ggcgcccggt
    26881 tcccaggcga ggtgatgcgc ctcgtcgacg atcagcaggt cccagccggc ttcgagtgcc
    26941 tgcgtctgac ggcgctcgcg cccgaccagc cagtcgcggc cgcagagcac cagctgttcg
    27001 gccaggaagg gattgtcgag gccctcgcgt tcgagttcga ggcagcgttc ctcgtcgtag
    27061 acggcgcagc cgagctggaa gcgccggcgc agttcgagga accactggtg gatcagcggt
    27121 tcgggcacga cgatcagcgc ccggccggcg cgcccggtca cgagctggcg gtgcaggatc
    27181 agaccggcct cgacggtctt gccgaggccg acctcgtcgg cgagcagcac gcgcggtgcg
    27241 tagcggccgg cgacctcgtg cgcgatccag agctggtgcg cgagcaggtc gatgcgcgca
    27301 ccggcgaggc cgcgcagttc cgaggcgcgc gcctgcgcgg cccaggccag cgcgtcgcag
    27361 cgcagcgtga acaggcgctg ggcgtcggtc tggttggcga acagccgctc ctgcggcttg
    27421 ctgaagctga tgaagttcga cagcccggtc tcgggcagca cgcggccgtc gcgcccgacg
    27481 taggcgaaca gcccgcgctc ttcgagcacc tcctcgacct gcagcgtcca gccctcgtgg
    27541 ctctgcaccg tatccccggg ctggaagcgc acgcgcgtca gcggcgcgcc ctggcgcgcg
    27601 tagacgcggg tttcgccgct gccgtggaac agcagcgtca cgcgccgggc atcgcccgac
    27661 agcaccatgc ccaggcccag ttccggttcg gtttcgctga tccagcggct gccgccgacg
    27721 aagtcgttca agtccgctca ctcctggcgc atgaaaaggc ggcggatgat aacgccgtgc
    27781 tgcccgtgac tcgaccgccg gccggttagc gactacatca gagggcttgc ccagccgcag
    27841 cattgcgcca ccccgcctgc cgcatgccgg agtccgtccg atgcgcctga ccctcgatgc
    27901 cctcgccgtg ctcgatgcca tcgcccgccg cggcagcttc gcggccgctg ccgatgagct
    27961 gcaccgcgtg ccctcggcga tcacctacac cgtgcacaag ctcgaacagg atctcgacgt
    28021 gcaggtgttc gaccgcagcg gccaccgcgc gcgcctgacc ccggtcggcg agaccctgct
    28081 cgccgagggg cgtcacctgc tgcgcgccgc cggcgagctc gaatgcctgg tgcggcgcgt
    28141 cgccaccggc tgggaggcgg agctgtcgat cgcgctcagc gcgctgctgc cgctcacgcc
    28201 gctgctgccg ctggtcgagg ccttcgatcg cgagactccc ggcacgcgcc tgcgtctggg
    28261 caccgaggtg ctcggcggcg tctgggatgc gctggtcacc ggccgcgccg atctggcgat
    28321 cggcgcgagc ggcgacgggc cggtcggcgg cggctacgcg agccgtgccc tcggcacgat
    28381 cggcttcgtg ttcgtgtgcg cgccgcagca tccgctggcc cgcagcgagg tgccgctcgg
    28441 cgaggagacc atcctggcca gccgcgcgat cgccgtcgcc gacagctcgc gggtgctggc
    28501 cccgcgcacg gcggcgctgc tcagcggcca gccggtgctg accgtgccga gcatggaggc
    28561 caagatcgac gcgcatcgcg ccgggctcgg cgtcggctac gtgccgcgcg cctgggtgcg
    28621 cgaggacctc gccgccggcc ggctgtgcga gtgtgcggtc gagcatccgc tgccgccggc
    28681 accgctgtat ctcgcctggc gcaccggtaa cggcggacgc ggcctgaagt ggttcctgcg
    28741 ggcgctggag gaactcgcgc cgtggcgcga ctggctcgat gccgaatgat ggtcgtggcc
    28801 ggacgcgcta acctcaagct cccgtccgcc ccttcatgct gtggtggaat ctgccgtcct
    28861 gccgggagcc acggtcttgg ccagcgtcca gcgcctgccc gccttcttcg atcacgccga
    28921 gcgcatccgc cgcgaggccg cgcgttacct gttcgattac ttcgaggctt gctgcgaagg
    28981 cgcgatggcg gtcgatcgcg aggcgcgcat cgtctggatc aatgagccct acgcgcaggc
    29041 gctcgggctc gcggacgcgg ccgccgcgct cggcaagccg gtcgaggatg tcatcccgca
    29101 cagcctgatg cgcgaggtcg tgcagagcgg ccggccgatc ctgctcgaca tcatgcagca
    29161 cggtgcgcgc tcgctggtgg tcacgcgcct gccgctgacc gacgagcacg gcgccgtcat
    29221 cggcgcggtc ggcttcgtgc tctacgacga tccgcgctcg ctgcagccgc tgatgaccaa
    29281 gttctcgcgc ctgcgcgccg agctcgacgc cgcccggcgc gagctcgccg ccagccgccg
    29341 cgcccgctac cggctcgatg actacgtcgg cagcagcccg gcggcgctgg aggtcaagcg
    29401 ccaggcggcg cgcgccgccg ccagcgattc gaccgtgctg ctgctcggcg agaccggcac
    29461 cggcaaggaa ctgatcgcgc aggcgatcca tgccgcctcc gcgcgcgccg cgcggccgtt
    29521 cgtcggcatc aacgtcgcgg ccgtgcccga tggcctgctc gaagccgagt tcttcggcag
    29581 cgcgcccggc gcctataccg gcgccgatcg ccgcgggcgg gacggcaagt tcaagctcgc
    29641 cgacggcggc acgctgttcc tcgacgagat cggcgacatg cctgcgcacc tgcaggcgaa
    29701 gctgctgcgc gtgctgcagg agcaggagtt cgagccgctc ggctcgaacc gcgtcgagcg
    29761 catcgacgtg cgcgtgatcg ccgccaccag ccgtgatctg ccggcgcagg tcgccggggg
    29821 gcgctttcgc gccgatctgt ggttccggct gaacgtgctg ccgatccggc tgccgccgct
    29881 gcgcgagcgc cgtgacgatc tgccgctgct cgccggggcg ctgctcgacc ggctctcggc
    29941 gcgcgccggc ggcgtgcgcc gcgtgctcgg cgcctcggcg ctcgcggagc tggcgggcta
    30001 cgactggccc ggcaacacgc gcgagctcgg caacgtgctc gaacgcgcgg tgatgctgag
    30061 cgaccggcgc tgctacgagg ccgcggatct ggccggcctg ctgcgtccgc ccgcggctgc
    30121 ggccgcatcg gcgccggccg cccccgccgt gcagcgcctc gccgatgccg tcgccgaggc
    30181 cgagcgcacg gcgatccgtt cggcgctcgc cgtcagcggc ggacgcaagg tcgatgcggc
    30241 gaagctg
//
LOCUS       DAOQIE010000008        29181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_677_length_29181_cov_7.860331, whole genome shotgun sequence.
ACCESSION   DAOQIE010000008 DAOQIE010000000
VERSION     DAOQIE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 29181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29181
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_29181_cov_7.860331"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1548)
                     /locus_tag="PK144_01015"
     CDS             complement(121..1548)
                     /locus_tag="PK144_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF64330.1"
                     /translation="MARSKKAESSKRLFVLDTNVLMHDPTALFRFKEHDIHLPMVVLE
                     ELDRAKKGMSEVAHNVRQVSRFLDELIAGANREEIDRGLSLPSPVNLGRTCAPSGQLY
                     FQTRPTGQHLPATLPGNVPDNDILSFAIALSHEHPSRPVTLVSKDINLRIKATILGIH
                     SEDYRNDQALDDIDLLYSGSRELPADFWDTHSKAMDSWKEAGRTFYRVQGPELDEWHA
                     NQLLYLPGELQPSFLVRRLDAQGAIIELTRDFTTANHAVWGVTARNREQNFALNLLMD
                     PEIDFVTLLGAAGTGKTLLALAAGLAQTLDQNRFREIIMTRVTVPVGEDIGFLPGTEE
                     EKMTPWMGALMDNLEVLAATRDAPGGSEWGRQATADLLSSRIKIRSLNFMRGRTFQQK
                     YLIIDEAQNLTSKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKG
                     WSHSGHVTLTRGERSRLADRAAEIL"
     gene            complement(1632..3953)
                     /locus_tag="PK144_01020"
     CDS             complement(1632..3953)
                     /locus_tag="PK144_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64331.1"
                     /translation="MLTHEREHVLAGHRWRFQRAGGYDHVCLDTGADLVALPTLDQKL
                     WAALACPTRGVEFDARTLDLIDTDADGRIRAPEVLAAVDWALARLRDPDTLLAGGEAL
                     ALGAIDTRHEDGPDSGRLIHACACEVLRQLGRAEAGTIELDDVADTHRLYATIPFNGD
                     GVITAAAAGDDEALRAALLDVLQVCGGVPDRSGVDGIDRERLQGCFAALDALAAWRDD
                     GGSGPVRPLGADTAAAAEALAAVQAKIDDYFLRCRLAAWDAALLDALQQDAGERTLAE
                     ALKPVRDAEALAALPLDRAAPGRPLDFGAALNPAWLGRLQALRAQVLQPLLGDDRSLD
                     ESHWEALKARFAPWFAWQAAQPHDGPATRLAALPAEALARLRDPDLRARLQALVERDE
                     ALSGQFAALAALERLIRYRRDLHRLLDNFVSLREFYLRRHRAIFQIGTLYLDGRSCEL
                     CIRVGDIARHALLAAAGNVCLVYCDCRRQEPGSGETRQQAIAAALTDGEVDELAEGRN
                     GVFYDRAGLDWDASVVKIVRNPVSLRQAFWSPYRRFGQFLSTQIEKFANARAQDIDRQ
                     LAGSIGPAAAPAAANAAAPAPAAPAPARIGGFDIARFAGIFAALGLALGAIGTAIASV
                     VTGLLALSPWQLPLVFAGVVLAISGPSLLMAWLKLRQRSLGPLLDATGWAINARARLG
                     PRFGRSLTALPKLPAHAERLRSDPFAERTPVLRIGLLLLLLGVLAVGGWRAGWYDAAL
                     LDLRRWVLAHTPKPAAATVPAPAPPAAPAPAPR"
     gene            complement(3997..4479)
                     /locus_tag="PK144_01025"
     CDS             complement(3997..4479)
                     /locus_tag="PK144_01025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNF64332.1"
                     /translation="MERTGIGQTVAPFTAAATGGETVDSAALRGRWLLLYFYPKDATP
                     GCTSEGRDFRELHPEFLAAGAVLYGVSRDSLASHEKFRAKEGFPFALISDPDETLCRL
                     FDVIRPKKLYGKDVIGIERSSFLIDPDGVLRREWRKLRVEGHARAVLAALREELAATG
                     "
     gene            complement(4485..5015)
                     /locus_tag="PK144_01030"
     CDS             complement(4485..5015)
                     /locus_tag="PK144_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HNF64333.1"
                     /translation="MKNYLVINVLGEDRPGLLLDLSRIILDCGCSIQDSYITVFGLEC
                     VAMLLVHGNWNTLAKLETQLKRAEEEHGLTITIKRTGPRKISQDALPYSVEVIALEAP
                     GIVNHLAAFFASRSINIEELSTRSYSAPHTGSPMYSVNMAIGIPASLHVALLREEFLD
                     YCDELNLDAVMEPVKG"
     gene            5178..6053
                     /gene="dapA"
                     /locus_tag="PK144_01035"
     CDS             5178..6053
                     /gene="dapA"
                     /locus_tag="PK144_01035"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNF64334.1"
                     /translation="MFHGSMVALVTPMSADGALDFDALARLVEFHIENGTDALVAVGT
                     TGESATLDVPEHIEVVRRVVKQVAGRVPVIAGTGANSTREAIELTRLAMEAGADACLL
                     VTPYYNKPTQEGLYRHYRAIAEAVPIPQILYNVPGRTACDMKPETVARLADIPNIVGL
                     KEASGSLERNRELIARCAGRIDLYSGDDDLGCASILAGFRGVISVTANVAPRAMHEMC
                     KAALAGDAASAEAINADLTGLHKQLFIESNPIPAKWALQRLGLIPSGIRLPLTPLSES
                     CHEAVLDAMRQAHVL"
     gene            6087..7286
                     /gene="bamC"
                     /locus_tag="PK144_01040"
     CDS             6087..7286
                     /gene="bamC"
                     /locus_tag="PK144_01040"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNF64335.1"
                     /translation="MPKLHTPLRVAAAGTTLALLAACSSPSGSLIPDRRPDYRQAREA
                     PTLELPPDLTSSTIDDTLKVPELNPGRQANLSDYNRERGVAGGAVASTAAGTAPRLVK
                     TESVLLQPEGMRIERQGEQRWLVVDAPADKVWPKVREFWTSNGFLLKRDDPRIGLLET
                     DWAENRADIPQDAIRALISKALDFAYSAPTRDKFRVRLERTGDGSGTAVYLTHYGAKE
                     VSRGRDNESTVWEPRPEDPELEAELLSRLMVFLGASEERARAQAGVGQKAAPVQKALT
                     RPGELGGVPGLFVTQDYSRAWRSVGAALDGGDYGIEEQDRARGLYIVEYVDRAGENRK
                     ADDNKGWFSGWFGSKSAPQPRGTRYQVRLADQGGETFVIVQNASGTPDNSAGARTLLE
                     TIARGVR"
     gene            7369..9213
                     /gene="mutL"
                     /locus_tag="PK144_01045"
     CDS             7369..9213
                     /gene="mutL"
                     /locus_tag="PK144_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNF64336.1"
                     /translation="MPIRRLPPELVNQIAAGEVVERPASVVKELLENSLDAGATRIDI
                     DIEQGGVRLIRVRDDGCGITREELAAALASHSTSKISTAADLDAIASLGFRGEALPSI
                     AAVSRLTLASRTDGAASGWQLAGDGREQPGEPLPAALAARGTAIEVRDLFHNVPARRK
                     FLKAERTEFGHIDELVRRLAASRPAVAFTLSHNGRALLRLPAAADRAGEERRIAELAG
                     SDFLGAALHVDHTAAGLRLHGWVGLPTAARAQADRQFFYVNGRMVRDRLVAHALRQAY
                     ADVLFHGRHPAFVLQLELDPQLVDVNAHPQKFEVRFREARLVHDFLFRTLHEVLADTR
                     AGAGAGAPAAVGVAAGALPDPEMPVPPDAGRWPPPAAPAPQQNRLALGAHESFAVYAR
                     LLDGLAPAPAQAGLAAVEPDATAAPPPAVPPLGYALAQLHGIWILAENAAGLVLVDMH
                     AAHERVTYERLKRAHAAEGLRTQPLLVPFGFAVGAREAAAIEAHAEVLAGLGLGIVLA
                     GPDRALLRELPALLADGDIEALVRGVLADLAEHGSSSRVGERHNELLATMACHGSVRA
                     RRRLTLAEMNALLRAMEATERSDQCNHGRPTWVQLGLGELDRLFLRGR"
     gene            9216..10166
                     /gene="miaA"
                     /locus_tag="PK144_01050"
     CDS             9216..10166
                     /gene="miaA"
                     /locus_tag="PK144_01050"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNF64337.1"
                     /translation="MTADPRPLAVCLMGPTASGKTDLAIELLERAPGGFEIVSVDSAM
                     IYRGMDIGTAKPDAGTLARAPHRLIDLVDPAESYSAGRFRRDALAAMDEIRAAGRVPL
                     LVGGTMFYFRALAQGLAELPEADAGVRAALDAELAAHGAAALHAQLAAIDPVAAARIH
                     PNDPQRVQRALEVWRLSGRTLSDWHACQHLAGCPYRLLNFVLLPADRGLLHARIEQRF
                     DRMLACGFIEEVARLRARADLHPGLPAMRAVGYRQVWEHLDGLTDRTELRQRGIQATR
                     QYARRQLTWLRGETGAVPLAPATAVNTVLRTLGALRDHPG"
     gene            10246..10500
                     /gene="hfq"
                     /locus_tag="PK144_01055"
     CDS             10246..10500
                     /gene="hfq"
                     /locus_tag="PK144_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNF64338.1"
                     /translation="MAKGHSLQEPFLNALRKERIPVSIYLVNGIKLQGQIESFDQFVV
                     LLKNSVSQMIYKHAISTVVPARNVRLHLAEDAGADGHDDA"
     gene            10542..11834
                     /gene="hflX"
                     /locus_tag="PK144_01060"
     CDS             10542..11834
                     /gene="hflX"
                     /locus_tag="PK144_01060"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNF64339.1"
                     /translation="MFERPRAGERAVLVHLALNAQIDPREQAEFEELAKSAGADIVGQ
                     VSGTRRAPDARYFVGSGKAEEIRDRVAASGAELAIFDHELSPSQERNLEALLQCRVID
                     RTGLILDIFATRARSFEGKLQVELAQLEHLATRLVRGWTHLERQRGGIGLRGPGETQL
                     ETDRRLLAGRIRQIKSRLARVDRTREQGRAARRKADMPTVSLVGYTNAGKSTLFNALT
                     GAGVYAADQLFATLDPTLRRLDLPNVEPVVLADTVGFISRLPHELVAAFRSTLVETCE
                     ASLLLHVIDAADPERAELEKAVIEVLDEIGAAGVARLPVYNKIDLLDHGEPRIERDDA
                     GLPVAVWLSAAGGGGLQELRQAIAELLRADTLRGTLELGAGQARLRARLFALGAVLSE
                     EAGADGGCRLQVSASRKHLRQLCRQEGLVEGWLEAAVH"
     gene            11929..13179
                     /gene="hflK"
                     /locus_tag="PK144_01065"
     CDS             11929..13179
                     /gene="hflK"
                     /locus_tag="PK144_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNF64340.1"
                     /translation="MAWNEPGGNNRDPWGGGGRDQGPPDLDEVVRKLTDRLSGLLSGR
                     RGGGGGGADGGDDGSGGFGGGRGKLLLGAGAAALLLIWAVAGSFYIVEEGRRGVVVRF
                     GRYVETTMPGLRWHLPYPLERVDIVDVEQVRSEEIGYRSGGRQPASRPVPKEALMLTQ
                     DENIVDVRLAVQYQVKDPRAYLFRVADPDTTLRQAVESATREVIGRSSMDFVLTEGRA
                     DIVAQIRELAQAVLDQYHTGLRINSVALQDAQPPEEVQEAFGDAIKAREDEQRQKNEA
                     EAYANEIIPKARGQAARRLQEADAYKAQVIAQAEGEAARFTQLLVQYSKAPQLTRQRL
                     YLETMETVLDASSKVFIDLDNGGSPLLYLPLDRLLQRSGQPGATGTLLPAPGHDTGNG
                     NPGGAAAGSDPARLRDTGRTREVR"
     gene            13179..14042
                     /gene="hflC"
                     /locus_tag="PK144_01070"
     CDS             13179..14042
                     /gene="hflC"
                     /locus_tag="PK144_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNF64341.1"
                     /translation="MNPRILIAVAGVLAAMLLSASLFTVGEAQKAIRFRLGEIVDTDY
                     PPGLHFKWPLINNVRKFDGRVQTLDTEPERFLTAEKKNVIVDSFVKWRIADTARFYTA
                     VAGDPLQANLRLDQITKDSMRSEFSKRTVQDVVAGDRRQIMDILSTTLRREAANLGVE
                     VVDVRIKRIDLPPDVSSSVFRRMRAERERVARDFRSRGAEAAERIRADADRQSTVLLA
                     EAYRDAERVRGEGDARAAGLYANAYGQDPDFFAFYRSLGAYRASFASKDDVIVLSPDS
                     EFFRYFKKPQP"
     gene            14147..14332
                     /locus_tag="PK144_01075"
     CDS             14147..14332
                     /locus_tag="PK144_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNF64342.1"
                     /translation="MWHDLLSAFGLMLVMEGVVPFLSPQALRTALVRLASLSDRELRV
                     GAALSMALGVAVLYWIR"
     gene            14371..15570
                     /locus_tag="PK144_01080"
     CDS             14371..15570
                     /locus_tag="PK144_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNF64343.1"
                     /translation="MTVKYRWLLPEGIEEILPAGARRVERLRRDLLDLYGRWGYELVM
                     PPFIEFLDALLTGFGRDLELETFKLTDQLSGRLLGVRADMTPQVARIDAHQLARAQPN
                     RLCYLGTVLRTRGDGFGGSRSPLQIGAELYGHAGPESDIEIIALMLETLRLARVEGVH
                     LDLGHVGLFRALARHAGLDERAEGELFDMLQRKSVPDIRAWLDETGLDAAHGEHFLRL
                     ASLNGGIEVLDAAGTALAGLGPEVGAALDALRRTAAVIARRYPGLPIHVDLAELRGYH
                     YHTGLVFAAYLPQFGSEVARGGRYDGIGSAFGRSRPATGFSADLKALLELGEGELPAV
                     AAIWAPAEDDAALEARIAALRNAGERVIHALPGAEDGGPRALGCDRVLVRRGSDWHIE
                     DIGPVAG"
     gene            15623..16915
                     /locus_tag="PK144_01085"
     CDS             15623..16915
                     /locus_tag="PK144_01085"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNF64344.1"
                     /translation="MGKNVVVIGTQWGDEGKGKIVDLLTERAGSVVRFQGGHNAGHTL
                     VVGGRKTVLHLIPSGILRAGVQCLIGNGVVLAPDALLAEIDQLEAAGIDVSGRLKLSE
                     ACTLILPYHVALDRAREQARGTRAIGTTGRGIGPAYEDKVARRALRLGDLFHRERFAA
                     KLGEILDFHNFVLANYFHAERVDFQKTLDDALTMAERLKPMVADVTQLLAEAGARGEN
                     VLFEGAQGALLDVDHGTYPFVTSSNTTAGGAATGTGMGPRYLDYVLGITKAYSTRVGG
                     GPFPTELDDEVGEHLGTKGHEFGATTGRRRRCGWFDAVALRRSIQNNSVSGLCITKLD
                     VLDGLSTLKICTGYNLDGRIVDYPPLGAEAFEVCEPVYEEMPGWSESTFGIQSLADLP
                     AAARAYLKRLEALTGAPIAIVSTGPDREETIVLQDPFA"
     gene            17001..17489
                     /gene="gpt"
                     /locus_tag="PK144_01090"
     CDS             17001..17489
                     /gene="gpt"
                     /locus_tag="PK144_01090"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNF64345.1"
                     /translation="MPEARRMLFPVSWDELHRNTRQLAWRLLERGPFTGIVAITRGGL
                     IPAGIIARELDIRLIETFCIASYDHARPDAEAGEAVLLKGFTAAAGGAGWLVIDELVD
                     TGRTMQLLRRHAPEALYAAVYAKPLGRPQVDLYVTEVSQDTWIQFPWDTEIQYAKPLV
                     ER"
     gene            17701..18753
                     /locus_tag="PK144_01095"
     CDS             17701..18753
                     /locus_tag="PK144_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNF64346.1"
                     /translation="MSVFTDPDFDGHEQVVHCHERASGLHAIIAIHSTRLGPALGGIR
                     MYPYADEAGALNDALRLARGMSYKSALAGLPLGGGKSVILGDPRRDKTPALLHAMGEA
                     IERLGGRYVGAEDSGTAVADLQLMAQTTAHVTGLQDKPGLDGGVRSGDPSPATALGVF
                     TGIRAAVRHRFGREDLAGLRVAIQGVGSVGHRLAQLLHAAGATLFVSDVWAPNLERAL
                     REFGAEPVTPDRIHAAPVDVYAPCALGAVLDDVTIPQLCAAIVAGAANNQLAQARHGE
                     ALRARGVLYAPDYAINAGGIIDVCAERLGEDAAGVRARVERIGATLTAIFEQAESEGL
                     PTQLVADRLAERRLYG"
     gene            complement(18895..19839)
                     /locus_tag="PK144_01100"
     CDS             complement(18895..19839)
                     /locus_tag="PK144_01100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF64347.1"
                     /translation="MTLPVHSEADYRIAVLIPCYNEAVTIGEVVTDFRRALPDATIYV
                     FDNNSVDDTAAIAHSVGADVRTVRQQGKGNVVRRMFADVEADLYVMVDGDGTYDADSA
                     AAMVRKLIGEHADMVVGCRHTEERSAYRAGHRFGNRLLTGCVAMLFGRSFTDILSGYR
                     VFSRRFVKSFPALAAGFEIETELTVHALELRMPIGEFDTPYRSRPAGSTSKLSTYRDG
                     WRILMTILKLMKSERPLSFFGAVCALLLLLSVALAAPIVQTWLTTGLVPRFPTAILAT
                     GLALLGFLSLACGLILDTVTRGRQELKRLAYLACAAPH"
     gene            complement(19926..21284)
                     /locus_tag="PK144_01105"
     CDS             complement(19926..21284)
                     /locus_tag="PK144_01105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF64348.1"
                     /translation="MLLLGIPTVLSHSLYTGCMLSIAVSIAGGLLVYAFMRRHASMNA
                     ALVAFGMVMLSGTSILYASVFVPDNLTAVLMPGAAMAIFHAIETPRRRGVWLIGAGVL
                     TGLAYSSKDTALLMLPPLVLWILIGMRDVGPLRRLQAAALYGLAMLAVYLAEGAAYWW
                     VADDFFYRSHALTQVHNRTIEASAGALEFAKAGYWALLTLLVDWARNGPMLVLGALGA
                     LGVLLWNRPLAGFVLTGLFVTLYLIVGSSSLTRLVHLPIQDRYLLPCIPFAAIALGLA
                     LDAVGRRWPGRLDITGAATIPLLIALLATGVKSAAAEAGSITFADYYRNAADAIRILS
                     ANGRPIHVDSVLHHHLRHMLPPELHRQLVVFDEMPSLAPGYYMIVPMLLPESHASKET
                     QMQLERMSPALPALIASIVTQPVVMRITTSHFDAGRYSATARDQPLMRAYKAAIVHAV
                     GP"
     gene            complement(21981..22511)
                     /locus_tag="PK144_01110"
     CDS             complement(21981..22511)
                     /locus_tag="PK144_01110"
                     /inference="COORDINATES: protein motif:HMM:NF023363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HNF64349.1"
                     /translation="MNRSACIVRSLALSAALAAAGCASVQPPETPDYLKPPADQSAYL
                     MTRGSGDYLYECRATPGAPKGHAWQFKATEATLSDADGKPVGRIGAGPLWTAADGSRF
                     TGVPQARKPVEQDKAVPWLLLGTKDNSGDGVFGRTKSVQRVDTEGGLFPNAPCTRRQA
                     GQIARVPYKATYYYYR"
     gene            22773..23570
                     /locus_tag="PK144_01115"
     CDS             22773..23570
                     /locus_tag="PK144_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNF64350.1"
                     /translation="MTVSLSRLLSSDDPLPVSVLNPGAHSPLLLLCDHASHAVPAALA
                     GLGLPAAELTRHIGWDPGAALVSAHLAGRFDAAAVLSGFSRLVIDCNRAPGDPTSIPE
                     ISDGTVVPGNRGLDEAAVLARLDACFWPYHQVITARIAQMRSDGRVPVVVAVHSFTPC
                     LNCAGGARPWQIGVLWNHDPRLAVPLIETLRAQGVCVGDNEPYSGRDSSYTVETHAAA
                     AGLPHVLIELRQDLLGDPAGCAHWADVLGDALAPLLARPELARAEFH"
     gene            23570..24709
                     /locus_tag="PK144_01120"
     CDS             23570..24709
                     /locus_tag="PK144_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNF64351.1"
                     /translation="MPGGPQPPFTLGIEEEYFLVERASRDLARELPPGLLDACNRRLG
                     DRVTPEFLRGQIEVGTRTCTSLAEARADLAELRTTLAEVAAQHGCAPIAASTHPFARW
                     HDQQPTDRARYRAIAEDLQGVGRRLLICGMHVHVGLPDDDLRIDLMGQFAYFLPHLLA
                     LSTSSPFWRGEDTGLCSYRVSVFNEMPRTGLPPAFASWGEYARHVRVLVGAGLIEDAT
                     KLWWDLRPSARFPTLEMRITDVCPRLDDALALAALTRCLLRRLWRLRRDNQRWRVYKR
                     MLIDENRWRAQRYGLAEGLVDFGRGCIVPFPELLAELQALVAEDAAHFGCEAEIRHLG
                     TILERGTGADRQRAVRLAALERGAAPLAAMQAVVDDLIVQTQVTG"
     gene            24788..27175
                     /locus_tag="PK144_01125"
     CDS             24788..27175
                     /locus_tag="PK144_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF64352.1"
                     /translation="MPIRSETLLPPGCLRRTTDAGTLGLSTTAGLEPLPLLFGQERAL
                     AAIDFGVRMGGDGYNLFLLGPGGIGKRRLARRVLNARADAEATPGDWVYVHNFSEPLK
                     PCALGLAPGQGPAFREALKTLVGELADLLPDLYEGEDCRARRQALKAEFVARDEERVK
                     SLRERARAQDVALTQTPDGFSLVPLADGEPMPDEDYEALAEDARAAVDAAMSEVRGAL
                     EDLLDTAPREQRAYRERQRALRQALMRREVEPRIAQLREAWSGLPAVLAHLDALAADL
                     IEHVDDFLPADDEDEERPPARQLKRYRVNVVVTRTPGAGAPVVYEDHPLHQNLLGRLE
                     YAAESGNLLTDFTLIRAGSLHTANGGYLILEARQLLSQPFAWEGLKRALLSRAIRIVS
                     PGELYGQVTTVSLDPEPIPLAVKVLLLGDAEVYELLSQLDPDFPALFKVVADLEPDVP
                     RDAASERLYARLIATIVHEAGLRPFAADALARVIDEAARQAEDAARLSLHLAGLSDLL
                     READFVAAETGTDTVTAAQVAEALARRRRRRGGIEARMLEAIRRGTVLIDTHGAAIGQ
                     VNGLAVLDLGDVRFAKPVRLSATVRLGSGEVVDIERESELGGAIHAKGVLILTSLLGA
                     RYLPDHPLSFRASLVFEQSYGPVDGDSASVAECCALLSALAKLPLAQHLAITGSLNQY
                     GRVQAIGGVNEKIEGFWAVCRDAGLTGTQGVIVPAANVQHLMLDPEVVEAVAAGRFHI
                     HAVAELDEALALLTGVAAGERDAQGRFPPASVNGRVEARLEAFALAAQRSGGARD"
     gene            27287..>29181
                     /locus_tag="PK144_01130"
     CDS             27287..>29181
                     /locus_tag="PK144_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64353.1"
                     /translation="MRPRTLIRHPALLPLLVFAALAACILGTGIGLYRWQHAQTEDEA
                     QRMLLAVADLKSRQIGDWLFERRADAASLAANRLVDTMIGDWLAGGTPQDARHAQLLA
                     RLRSFRDLMGYANVLLTDPQGRIVLAAENWPQDESGWLRERVPPLLAQGRTMVSDLHR
                     LDAADRPLLMEVVTPVLAGGGEAQRVVAGLVLLIDPRSYLYPVVQGWPTASASAEALL
                     VRVEGDDIVYLSPLRHDSVAPLGLRRPLDESGLVAAQAARGLRGITRGHDYHDAPVLA
                     AVRAIEDTPWLLIVQIGAEEVFAPLRRAAWLVGLTLGLLLAAAAAVTSVWMWWLRLRE
                     RLHAAGLEAEGMRLRSELEFLSNHANDIILLADDEGHVLRANDRAVEAYGYSHDELTG
                     LSLAALRAQEAPAEAQTLLPGSGTVYETVHRRRDGSCFAVEVSTRSFRVAATRYRQSI
                     IRDISERKVAEALLQRQTQLYALLTATNEAIVRAGDVRALYREVCAAACRSGGFVTAW
                     IGTIGSDGNRIEVAASAGSAEVVAYVDRLGLVLDASRPEGRGPTATAIREGCSMLSNT
                     IGDDSPMAFWQQAAEHFGIGSAACFPLRCAGHTVAGISLYAPVPGYFRDDLLHLLSRL
                     RVALRFL"
BASE COUNT         4162 a        10329 c        10452 g         4238 t
ORIGIN      
        1 cgacgggatg cagcgacacg cctgtgggtc gggcttcagc ccgacggggt ctgtgccgat
       61 gcgatggaaa ggcgtcggcc tgaaggccga cccgcaatcc ctccggcccg ccgcctcgcg
      121 ctagagaatc tccgcggcgc gatcggcgag tcgagagcgt tcgccgcgcg tgagcgtcac
      181 gtggcccgaa tgcgaccagc ccttgaagcg gtcgacgacg taggtgaggc cggaactccc
      241 ttcggtgagg tagggcgtgt cgatctgcgc gatgttgccg aggcagacga ccttggtgcc
      301 ggggccggcg cgcgtgatca gcgtcttcat ctgcttcgag gtcaggttct gcgcctcgtc
      361 gatgatcagg tacttctgct ggaaggtgcg cccgcgcatg aagttcagcg agcggatctt
      421 gatgcgcgag gacagcaggt cggcggtcgc ctgccggccc cattcactgc caccgggcgc
      481 atcgcgggtc gcggccagca cttcgaggtt gtccatcagc gcgcccatcc acggcgtcat
      541 cttttcctcc tcggtgccgg gcaggaagcc gatgtcctcg ccgaccggca ccgtcacgcg
      601 cgtcatgatg atctcgcgga agcggttctg gtcgagcgtc tgcgcgaggc ccgcggcgag
      661 cgccagcagc gtcttgccgg tgccggcggc gccgagcagc gtcacgaagt cgatctcggg
      721 gtccatcagc aggttcagcg cgaagttctg ctcgcggttg cgcgccgtca cgccccagac
      781 cgcatggttg gcggtcgtga agtcgcgcgt cagctcgatg atcgcgccct gcgcatcgag
      841 gcggcgcacg aggaagctcg gctgcagctc gcccggcagg tacagcagct ggttcgcgtg
      901 ccactcgtcg agttccggcc cctgcacgcg gtagaacgtg cgcccggcct ccttccacga
      961 gtccatcgcc ttgctgtgcg tgtcccagaa atccgccggc agctcgcggc tgccggagta
     1021 cagcaggtcg atgtcatcga gcgcctggtc gttgcggtag tcctcgctgt ggatgccgag
     1081 gatcgtcgcc ttgatgcgca ggttgatgtc cttggacacc agcgtcaccg gccggctcgg
     1141 atgctcgtgc gacagcgcga tcgcgaagct caggatgtcg ttgtccggca cgttgccggg
     1201 cagcgtcgcc ggcaggtgct ggccggtcgg gcgggtctgg aagtacagct ggccgctcgg
     1261 cgcgcaggtg cgcccgaggt tgaccggcga gggcagggac aggccgcggt cgatctcctc
     1321 gcggttggcc ccggcgatca gctcgtcgag gaagcggctg acctgccgca cgttgtgcgc
     1381 aacctcggac atgcccttct tggcgcggtc gagttcttcg agcaccacca tcggcaggtg
     1441 gatgtcgtgc tccttgaagc ggaacagcgc ggtcgggtcg tgcatcagga cgttggtgtc
     1501 gagcacgaac agacgcttcg aggactcggc tttcttcgat ctggccatga cggaccccgg
     1561 gcggtgatgg ttgccccgac tgtatccgcc gccgccgcgg cgcgcgagcc gggaccgcgg
     1621 ggcgggccgg atcagcgcgg cgccggggca ggggcggccg ggggcgcagg cgcgggcacg
     1681 gttgcggcgg ccggtttcgg cgtgtgcgcg agcacccagc ggcgcaggtc gagcagcgcc
     1741 gcgtcgtacc agccggcgcg ccagccgccg acggcgagca ccccgagcag caggagcagc
     1801 agcccgatgc gcagcaccgg ggtgcgctcg gcgaacggat cgctgcgcag gcgctcggcg
     1861 tgcgccggca gcttcggcag cgcggtcagc gaacggccga agcgcggccc gagccgggcg
     1921 cgggcattga tcgcccagcc ggtggcatcg agcagcggtc cgaggctgcg ctggcgcagc
     1981 ttcagccagg ccatcagcag cgacggcccg gagatcgcca gcacgacgcc ggcgaacacc
     2041 agcggcagct gccaggggct gagcgccagc aggccggtga ccaccgacgc gatcgccgtg
     2101 ccgatcgcgc cgagcgccag gccgagcgcg gcgaagatgc cggcgaagcg cgcgatgtcg
     2161 aagccgccga tgcgggccgg tgcgggcgct gcgggggcgg gcgcagccgc attcgccgct
     2221 gccggggccg cagccggccc gatgctgccg gccagctgcc ggtcgatgtc ctgcgcacgg
     2281 gcgttcgcga acttctcgat ctgcgtgctc aggaactgcc cgaagcgccg gtacggcgac
     2341 cagaacgcct gccgcaggct gaccgggttg cgcacgatct tgacgacgct cgcatcccag
     2401 tcgagcccgg cgcggtcgta gaacacgccg ttgcgaccct cggccagctc gtcgacctcg
     2461 ccgtcggtca gcgccgcggc gatggcctgc tggcgggttt cgccgctgcc gggctcttgc
     2521 cggcggcagt cgcaatagac gaggcagacg ttgccggcgg cggcgagcag cgcatggcgg
     2581 gcgatgtcgc cgacgcggat gcagagctcg cagctgcgcc cgtcgagata cagcgtgccg
     2641 atctggaaga tcgcgcggtg gcggcgcagg tagaactcgc gcagggacac gaagttgtcg
     2701 agcagccggt gcaggtcgcg ccggtagcgg atcagccgct ccagcgccgc gagcgccgcg
     2761 aactggccgg acagcgcctc gtcgcgctcg accagcgcct gcaggcgcgc gcggaggtcc
     2821 ggatcgcgca gccgcgcgag cgcctcggcc ggcagcgccg ccagccgggt ggcggggccg
     2881 tcatgcggct gcgcggcctg ccacgcgaac cacggtgcga agcgcgcctt cagggcttcc
     2941 cagtggctct cgtcgagcga gcggtcgtcg ccgagcagcg gctgcagcac ctgcgcgcgc
     3001 agcgcctgca gccgtccgag ccaggccggg ttcagcgccg ccccgaaatc gagcgggcgt
     3061 ccgggggcgg cgcgatcgag cggcagcgcc gcgagcgcct cggcatcacg caccggcttc
     3121 agcgcctcgg cgagcgtgcg ctcgccggca tcctgctgca gcgcgtcgag cagcgcggca
     3181 tcccaggcgg cgagccggca gcgcaggaag tagtcatcga tcttggcctg cacggcggcg
     3241 agcgcctcgg ccgcggcggc ggtgtcggcc ccgagcgggc gtaccggtcc gctgccgccg
     3301 tcgtcgcgcc aggcggcgag cgcatcgagg gcggcgaagc agccctgcag gcgctcgcgg
     3361 tcgatgccgt cgacgccgct gcgatccggg acgccgccgc agacctgcag cacgtcgagc
     3421 agcgcggcgc gcagggcttc gtcatcgccg gcggcggctg cggtgatcac gccgtcgccg
     3481 ttgaacggga tcgtcgcgta cagccggtgc gtgtcggcga catcgtcgag ctcgatcgtg
     3541 ccggcttcgg cgcgcccgag ctggcgcagc acctcgcacg cgcaggcgtg gatcagccgg
     3601 ccgctgtcgg ggccgtcctc gtggcgcgtg tcgatcgcgc ccagcgccag tgcctcgccg
     3661 ccggcgagca gggtgtcggg gtcgcgcagc cgtgcgagcg cccagtcgac ggcggcgagc
     3721 acttccggcg cgcggatgcg gccgtcggcg tcggtgtcga tcaggtcgag cgtgcgcgcg
     3781 tcgaactcga cgccgcgcgt cgggcaggcg agcgcggccc agagcttctg gtccagcgtc
     3841 ggcagcgcga cgaggtcggc gccggtatcg agacagacat ggtcgtaacc gccggcccgc
     3901 tggaaacgcc agcgatgacc ggccagcacg tgctcgcgct cgtgcgtcag catgggcagg
     3961 actccgcgac cggatgaagg atcgcgcggg cagacctcag ccggtggcgg cgagttcttc
     4021 gcgcaaggcg gcgagcacgg cgcgggcgtg gccctcgacc ctgagcttgc gccattcgcg
     4081 gcgcagcacg ccgtcgggat cgatcaggaa gctgctgcgc tcgatgccga tcacgtcctt
     4141 gccgtagagc ttcttcggcc ggatcacgtc gaacagccgg cagagggttt cgtcggggtc
     4201 cgagatcagc gcgaacggga agccttcctt cgcgcggaat ttctcgtgac tcgccagcga
     4261 atcgcgcgag acgccataga ggacggcgcc ggcggcgagg aattccgggt gcagctcgcg
     4321 gaagtcgcgg ccctcgctgg tgcagccggg ggtcgcgtcc ttcggataga agtacagcag
     4381 cagccagcgt ccgcgcagcg ccgccgaatc gacggtttcg ccgccggtcg cggcggcggt
     4441 gaacggtgcc acggtctggc cgatgccggt gcgttccatc gccttcagcc cttgaccggt
     4501 tccatgaccg cgtcgaggtt cagctcgtcg cagtagtcga ggaattcctc gcgcagcagc
     4561 gccacgtgca ggctggccgg gatgccgatc gccatgttca ccgagtacat cgggctgccg
     4621 gtgtgcgggg ccgagtagct gcgcgtcgag agctcctcga tgttgatgct gcgcgaggcg
     4681 aagaaggccg cgaggtgatt gacgatgccc ggcgcctcca gcgcgatgac ctcgacgctg
     4741 tacggcaggg cgtcctgcga gatcttgcgc gggccggtgc gcttgatcgt gatcgtcagg
     4801 ccatgttcct cctcggcgcg cttgagctgg gtttcgagct tcgcgagcgt gttccagttg
     4861 ccgtgcacca gcagcatcgc cacgcattcg aggccgaaca ccgtgatgta gctgtcctgg
     4921 atgctgcagc cgcagtcgag gatgatgcgc gacaggtcga gcagcagacc cgggcgatcc
     4981 tcaccgagca cgttgatgac gagatagttc ttcacggggg cgcgcatgcg tcgggggcag
     5041 tggactgggg tgcgatgagg cggacggggc agtgtagcgg ctcgccgggg gtgtgcaagc
     5101 cgcgttccgg ccggcgcttg tgaccggcac cggcggcacg taccatgccg cacattttcc
     5161 ttgcggggtt cccggacatg ttccacggca gcatggtcgc gctggtcacg ccgatgtcgg
     5221 cggacggcgc cctcgacttc gatgcgctcg cacgcctcgt cgagttccac atcgagaacg
     5281 gcaccgacgc gctggtcgcg gtcggcacca ccggtgaatc ggcgacgctc gacgtgcccg
     5341 agcacatcga ggtcgtgcgc cgcgtcgtga agcaggtggc cgggcgcgtg ccggtgatcg
     5401 ccggcaccgg cgccaactcg acgcgcgagg cgatcgagct gacccggctg gccatggagg
     5461 ccggtgccga cgcctgcctg ctggtgaccc cgtactacaa caagccgacc caggaaggcc
     5521 tgtaccggca ctaccgcgcg atcgccgagg ccgtgccgat cccgcagatc ctctacaacg
     5581 tgcccgggcg caccgcctgc gacatgaagc ccgagaccgt cgcgcgtctg gccgacatcc
     5641 cgaacatcgt cggcctcaag gaggcgagcg gctcgctgga gcgcaaccgc gagctgatcg
     5701 cgcgctgcgc cgggcgcatc gacctctata gcggtgacga tgacctcggc tgcgcgagca
     5761 tcctcgccgg tttccgcggc gtgatctcgg tgaccgccaa cgtcgcgccg cgcgcgatgc
     5821 acgagatgtg caaggctgcg ctcgccggcg acgccgccag cgccgaggcg atcaacgccg
     5881 acctgaccgg cctgcacaag cagttgttca tcgaatccaa tccgattccg gccaagtggg
     5941 cgctgcagcg cctggggctg atcccgtccg gcatccgcct gccgctgacg ccgctgtcgg
     6001 agtcctgtca cgaagccgtg ctcgacgcga tgcgtcaggc ccacgtgctc tgaacatccc
     6061 gtccgccgcc gacgaaccct cgccccatgc ccaagctcca taccccgctg cgcgtcgctg
     6121 ccgccggcac gacgctggcc ctgctcgccg cctgctcctc cccgagcggc agcctgattc
     6181 ccgaccgtcg ccccgattac cgccaggccc gcgaggcgcc gacgctggaa ctgccgccgg
     6241 acctgaccag ctcgacgatc gatgacacgc tgaaggtgcc ggagctgaac ccgggccggc
     6301 aggcgaacct ctccgactac aaccgcgaac gcggcgtggc cggcggagcc gttgcgagca
     6361 ctgccgccgg cacggcgccg cggctggtca agaccgaatc ggtgctgctg cagcccgagg
     6421 gcatgcgcat cgagcgtcag ggcgagcagc gctggctggt ggtcgacgcg ccggccgaca
     6481 aggtctggcc gaaggtgcgc gagttctgga ccagcaacgg cttcctgctc aagcgtgacg
     6541 atccgcgcat cggcctgctg gagaccgact gggccgagaa ccgcgccgac atcccgcagg
     6601 atgccatccg cgcgctgatc tcgaaggcgc tcgacttcgc gtactcggct ccgacccgcg
     6661 acaagttccg cgtgcgcctc gaacgcaccg gcgacggcag cggcacggcc gtctacctga
     6721 cccactacgg tgccaaggaa gtcagccgcg gtcgcgacaa cgaatcgacg gtctgggaac
     6781 cgcgcccgga agacccggaa ctcgaagccg aactgctgtc gcggctgatg gtgttcctcg
     6841 gggcttccga ggagcgcgcc cgcgcccagg ccggcgtcgg ccagaaggcc gcgccggtgc
     6901 agaaggcgct gacccgtccg ggcgagctcg gcggtgtgcc gggcctgttc gtcacgcagg
     6961 actactcgcg cgcctggcgc agcgtcggtg cggcgctcga tggcggtgac tacggcatcg
     7021 aggagcagga tcgcgcccgc ggcctgtaca tcgtcgagta cgtcgatcgc gccggcgaga
     7081 accgcaaggc cgacgacaac aagggctggt tctcgggctg gttcggctcg aagtcggcgc
     7141 cgcagccgcg cggcacgcgc tatcaggtgc gcctcgccga tcagggtggc gagaccttcg
     7201 tcatcgtgca gaacgccagc ggcacgccgg acaactccgc cggggcccgc acgctgctcg
     7261 aaaccatcgc caggggtgtg cgctgagccg ccgcctgccc gcatcgtccc ctgcacgggc
     7321 cggccatccg ccggcccgtg gcatttccgg agctgcccgg agaccgccat gccgatccgt
     7381 cgcctgccgc ccgaactcgt caaccagatc gccgccggcg aggtcgtcga gcgcccggcc
     7441 tcggtggtca aggagctgct ggagaacagc ctcgatgccg gcgccacgcg catcgacatc
     7501 gacatcgagc agggcggcgt gcggctgatc cgcgtgcgcg acgacggctg cggcatcacc
     7561 cgcgaggagc tcgccgcggc gctggcgagc cactcgacca gcaagatcag cacggcggcc
     7621 gatctcgacg cgatcgcgag cctcggcttt cgcggcgagg cgctgccgag catcgctgcg
     7681 gtatcgaggc tgacgctcgc ctcgcgcacc gacggtgccg ccagcggctg gcagctcgcc
     7741 ggcgacggcc gcgagcagcc cggcgaaccg ctgccggcgg cgctggcggc gcgcggcacg
     7801 gcgatcgagg tgcgcgacct gttccacaac gtgccggcgc ggcgcaagtt cctgaaggcc
     7861 gaacgcaccg agttcggtca tatcgacgag ctggtgcgcc ggctcgccgc ctcgcggccc
     7921 gcggtcgcgt tcacgctgag ccacaacggc cgggcgctgc tgcgcctgcc ggcggcggcg
     7981 gatcgcgccg gcgaggagcg gcgcatcgcc gagctggccg gcagcgattt cctcggcgcg
     8041 gcgctgcacg tcgatcacac ggccgccggc ctgcgcctgc acggctgggt cgggctgccg
     8101 acggcggcgc gcgcgcaggc cgaccggcag ttcttctatg tcaacggccg catggtccgc
     8161 gaccggctgg tcgcgcacgc gctgcgccag gcctacgccg acgtgctgtt ccacggccgg
     8221 catccggcct tcgtgctgca gctcgaactc gacccgcagc tggtcgatgt caacgcccat
     8281 ccgcagaagt tcgaggtgcg cttccgcgag gcgcggctgg tgcatgactt cctgttccgc
     8341 acgctgcacg aggtgctggc cgacacgcgc gccggcgccg gcgccggcgc tccggctgct
     8401 gtgggtgttg cggccggcgc tctgccggat cctgaaatgc cggtcccgcc tgatgcgggt
     8461 cgctggccgc cgccggccgc gcccgcgccg cagcagaacc gcctcgccct cggtgcgcac
     8521 gagtcgttcg cggtctatgc gcgcctgctc gacgggcttg caccggcacc ggcgcaagcc
     8581 gggcttgccg cggtggagcc ggacgcgaca gccgcgccgc cgcctgcggt gccgccgctc
     8641 ggctatgcgc tcgcgcagct gcacggcatc tggatcctcg ccgagaatgc cgccgggctg
     8701 gtgctggtcg acatgcacgc cgcgcacgag cgcgtcacct acgagcgcct gaagcgcgcg
     8761 cacgcggccg aaggcctgcg cacgcagccg ctgctggtgc cgttcggctt cgcggtcggg
     8821 gcgcgcgaag cggcggcgat cgaggctcac gccgaagtgc tcgccggcct cggcctcggc
     8881 atcgtgctgg ccggcccgga ccgcgcgctg ctgcgtgagc tgccagcact gcttgccgat
     8941 ggcgacatcg aggcgctggt gcgcggcgtg ctcgccgatc tggccgaaca cggcagcagt
     9001 tcgcgcgtcg gcgagcgcca caacgagctg ctggcgacca tggcctgcca cggttcggtg
     9061 cgcgcccggc ggcgcctcac gctggccgag atgaatgcgc tgctgcgcgc gatggaggcc
     9121 accgagcgca gcgaccagtg caaccacggc cggccgacct gggtgcagct cggtctcggc
     9181 gagctcgacc ggctgttcct gcgcgggcgc tgagcatgac ggccgacccg cgtccgctcg
     9241 ccgtctgcct gatggggccg accgcctccg gcaagaccga tctcgcgatc gagctgctcg
     9301 aacgggcgcc gggcgggttc gagatcgtca gcgtcgattc ggcgatgatc taccgcggca
     9361 tggacatcgg caccgccaag cccgatgccg gaaccctcgc ccgcgcgccg caccggctga
     9421 tcgatctcgt cgatccggcc gaaagctact cggccggccg cttccggcgc gatgcgctgg
     9481 cggcgatgga cgagatccgc gccgccgggc gcgtgccgct gctggtcggc ggcacgatgt
     9541 tctatttccg ggcgctggcg caggggctcg ccgaactgcc cgaggccgac gccggcgtgc
     9601 gtgccgcgct cgatgccgag ctggcagcgc acggcgccgc ggcgctgcac gcgcagctcg
     9661 cggcgatcga tccggtggcg gcggcgcgca tccacccgaa cgatccgcag cgcgtgcagc
     9721 gtgcgctcga ggtctggcgg ctgagcggac gcacgctcag cgactggcac gcctgccagc
     9781 acctcgccgg ctgcccgtac cggctcctga acttcgtgct gctgcccgcg gatcggggcc
     9841 tgctgcatgc gcgcatcgaa cagcgcttcg atcgcatgct ggcctgcggt ttcatcgagg
     9901 aggtggcgcg gctgcgggcg cgcgccgacc tgcaccccgg cctgccggcc atgcgtgcgg
     9961 tcggctatcg tcaggtctgg gaacatctgg atggcttgac ggaccgtacc gagttgcggc
    10021 agcgtggaat ccaggcaacc cgccagtacg cccgaaggca gctgacgtgg ttgcgcggag
    10081 agaccggcgc cgttcccctg gcgccggcca cggccgtgaa cacggtgctc cgcactttgg
    10141 gtgcgttgcg ggaccacccg ggttgagcag gaagaatcac cggtcagcct gcgtcctgcc
    10201 cgggcaacag acagccagac ttcgataaca acaaggagcc atgacatggc taagggccat
    10261 tcgcttcagg aaccgttctt gaacgccttg cgcaaggagc gcatccccgt ctcgatctat
    10321 ctggtcaacg gcatcaagct gcagggccag atcgaatcct tcgaccagtt cgtcgtgctg
    10381 ctgaagaatt ccgtcagcca gatgatctac aagcacgcga tctcgaccgt cgtgccggcg
    10441 cgcaacgtgc gcctgcacct cgccgaggac gccggcgccg atggccacga cgacgcctga
    10501 acccgtcgcc ccgcacccct gcgtgaccga ggaccctgcc tttgttcgaa cgaccccgcg
    10561 cgggtgagcg cgcggtgctg gtgcacctcg cgctgaacgc gcagatcgat ccccgggagc
    10621 aggcggagtt cgaggagctg gcgaagtcgg caggcgccga catcgtcggc caggtgagcg
    10681 gcacacgccg tgcgccggac gcgcgctatt tcgtcggcag cggcaaggcc gaggaaatcc
    10741 gcgaccgggt cgcggcaagc ggcgccgaac tcgcgatctt cgatcacgaa ctgtcgccgt
    10801 cgcaggagcg caacctcgaa gcgctgctgc agtgccgggt catcgaccgc accgggctga
    10861 tcctcgacat cttcgcgacc cgtgcgcgca gcttcgaggg caagctgcag gtcgagctcg
    10921 cgcagctcga acacctggcc acgcgcctgg tgcgcggctg gacccacctc gaacgccagc
    10981 gcggcggcat cggcctgcgc ggtcccggcg agacccagct cgaaaccgac cgccggctgc
    11041 tggccggacg catccgccag atcaagtccc ggctcgcgcg cgtcgatcgc acgcgcgagc
    11101 aggggcgggc ggcgcggcgc aaggccgaca tgccgaccgt gtcgctggtc ggctacacca
    11161 atgccggcaa gtcgacgctg ttcaacgcgc tgaccggggc cggcgtctac gcggccgacc
    11221 agctgttcgc cacgctcgat ccgacgctgc gccggctcga cctgccgaac gtcgagccgg
    11281 tcgtgctcgc cgatacggtc ggcttcatct cgcgcctgcc gcacgagctg gtcgcggcct
    11341 tccgctcgac gctggtcgag acctgcgagg ccagcctgct gctgcacgtc atcgatgccg
    11401 ccgatcccga gcgcgccgag ctggaaaagg ccgtgatcga ggtgctcgac gagatcggtg
    11461 ccgccggcgt ggcgcggctg ccggtctaca acaagatcga cctgctcgat cacggcgagc
    11521 cgcgcatcga gcgtgatgat gccggcctgc cggtggcggt ctggctgtcg gcggccggcg
    11581 gtggcgggct gcaggagctg cgccaggcga ttgccgagct gctgcgggcc gacacgctgc
    11641 gcggcacgct cgaactcggt gccggtcagg cgcggctgcg cgcccggctg ttcgccctcg
    11701 gggcggtgct gagcgaggaa gccggcgccg atggcggctg ccggctgcag gtcagtgcct
    11761 cgcgcaagca cctgcgccag ctctgccgcc aggaaggtct ggtcgagggc tggctggaag
    11821 cggccgtgca ctagacgcac aaccccatgc gcacggagcg cctgccgggc cgctgcggcg
    11881 gccgcgagcg ctgctccgtg caaggtcccc attggctgga gcgagtacat ggcctggaac
    11941 gaaccgggtg gcaacaaccg ggacccctgg ggcggcggtg gccgcgatca gggaccgccg
    12001 gatcttgacg aagtggtgcg caagctgacc gatcgcctgt ccggactgct gtccgggcgg
    12061 cgcggcggcg gcggcggcgg cgcggacggc ggtgacgacg gcagcggcgg tttcggtggc
    12121 gggcgcggca agctgctgct cggtgccggc gcggcggcgc tgctgctgat ctgggcggtg
    12181 gccggcagtt tctacatcgt cgaggaaggc cggcgcggcg tggtcgtgcg cttcggccgc
    12241 tacgtcgaga cgacgatgcc gggcctgcgc tggcacctgc cgtatccgct ggagcgcgtc
    12301 gacatcgtcg atgtcgagca ggtgcgctcc gaggagatcg gctaccgctc cggcggccgc
    12361 cagccggcct cgcgcccggt gccgaaggaa gcgctgatgc tgacccagga cgagaacatc
    12421 gtcgacgtgc ggctggccgt gcagtaccag gtcaaggacc cgcgcgcata cctgttccgc
    12481 gtcgccgatc ccgacacgac gctgcgtcag gccgtcgaga gcgcgacgcg cgaggtcatc
    12541 ggcaggagtt cgatggattt cgtgctgacc gagggccgcg ccgacatcgt cgcgcagatc
    12601 cgcgaactcg cgcaggccgt gctcgatcag taccacacgg gcctgcgcat caacagcgtc
    12661 gcgctgcagg atgcgcagcc gcccgaggaa gtccaggagg ccttcggcga cgcgatcaag
    12721 gcgcgcgagg acgagcagcg ccagaagaac gaggccgagg cctacgccaa cgagatcatc
    12781 ccgaaggccc gcggccaggc ggcgcgccgg ctgcaggagg ccgacgccta caaggcgcag
    12841 gtgatcgcac aggccgaggg cgaggcagcg cgcttcacgc agctgctcgt gcagtactcg
    12901 aaggcgccgc agctcacgcg ccagcgcctg tacctcgaaa ccatggaaac cgtgctcgac
    12961 gcgagcagca aggtgttcat cgacctcgac aacggcggca gcccgctgct gtacctgccg
    13021 ctcgatcgcc tgctccagcg ctccggccag cccggcgcca ccggcacgct gctgccggcc
    13081 ccgggtcacg ataccggcaa cggcaatccg ggcggcgcgg cagccggcag cgatccggca
    13141 cgccttcgcg ataccggccg tacccgggag gtccgctgat gaatccccgc atcctgatcg
    13201 ccgtcgccgg cgtgctcgcg gcgatgctgc tgtcggcatc gctgttcacg gtcggcgagg
    13261 cccagaaggc catccgcttc cgcctcggcg agatcgtcga taccgactat ccgccgggcc
    13321 tgcacttcaa gtggccgctg atcaacaacg tgcgcaagtt cgacggtcgc gtgcagacgc
    13381 tcgacaccga acccgagcgc ttcctgaccg ccgagaagaa gaacgtcatc gtcgattcgt
    13441 tcgtgaagtg gcgcatcgcc gataccgccc gcttctacac cgcggtcgcc ggcgatccgc
    13501 tgcaggccaa cctgcgcctc gaccagatca ccaaggacag catgcgcagc gagttctcga
    13561 agcgcacggt gcaggatgtg gtcgccggcg atcgccgcca gatcatggac atcctgtcga
    13621 cgacgctccg gcgcgaggcg gccaatctcg gcgtcgaggt cgtcgacgtg cgcatcaagc
    13681 gcatcgacct gccgccggac gtgtcgagct cggtgttccg gcgcatgcgc gccgagcgcg
    13741 agcgcgtcgc gcgtgacttc cgctcgcgcg gcgccgaggc cgccgagcgc atccgcgccg
    13801 atgccgatcg ccagagcacg gtgctgctcg ccgaggccta ccgcgacgcc gagcgcgtgc
    13861 gcggcgaggg cgatgcgcgt gccgccgggc tgtacgcgaa cgcctacggc caggacccgg
    13921 acttcttcgc gttctaccgc agtctcggtg cctaccgcgc gagcttcgcc agcaaggacg
    13981 acgtgatcgt gctgtcgccc gacagcgagt tcttccgcta cttcaagaag ccgcagccct
    14041 gaggctgcgg gcgcgtcgcg tcgccacggg cgggccctcg caaggaggcc cgcccgttgc
    14101 attgcggccg cccgcgcgca ggggccgcgc gccagcggtg tgcatgatgt ggcatgatct
    14161 gctgtctgcc ttcggcctga tgctggtgat ggagggcgtc gtgcccttcc tgagtccgca
    14221 ggcgctgcgc acggcgctgg tgcggctcgc gagcctcagt gaccgcgagc tgcgcgtcgg
    14281 cgccgcactg agcatggcgc tcggcgtcgc cgtgctgtac tggatccgct gagcgcgcga
    14341 gcgcgcccgg cccgaccgga agtttcatgc atgaccgtca aataccgctg gctgctgccg
    14401 gagggcatcg aggaaatcct gcccgccggt gcgcgccgcg tggagcgcct gcgccgcgac
    14461 ctgctcgacc tgtacggacg ctggggctac gaactcgtga tgccgccgtt catcgagttc
    14521 ctcgatgcgc tgctgaccgg cttcggccgt gacctcgaac tcgaaacctt caagctgacc
    14581 gaccagctct ccgggcggct gctcggcgtg cgcgccgaca tgacgccgca ggtcgcgcgc
    14641 atcgacgcgc accagctcgc gcgggcgcag ccgaaccgcc tctgctatct cggcacggtg
    14701 ctgcgcacgc gcggcgacgg cttcggcggc tcgcgcagcc cgctgcagat cggcgccgag
    14761 ctttatggcc atgcggggcc ggagagcgac atcgagatca tcgcgctgat gctcgaaacc
    14821 ctgcggctgg cgcgcgtcga gggcgtgcac ctcgatctcg gccacgtcgg cctgttccgg
    14881 gcgctcgcgc gccacgccgg gctcgatgag cgcgccgaag gcgagctgtt cgacatgctg
    14941 cagcgcaagt ccgtgccgga catccgggcc tggctcgacg agaccgggct cgatgcggcg
    15001 cacggcgagc atttcctgcg gcttgcgagc ctcaacggcg gcatcgaggt gctcgatgcc
    15061 gccggcaccg cgctcgccgg cctcggcccg gaagtcggcg ccgcgctcga tgcgctgcgc
    15121 cgcaccgccg ccgtcatcgc ccgccgttac ccgggcctgc cgatccacgt cgatctcgcc
    15181 gagctgcgcg gttaccacta ccacaccggc ctggtgtttg cggcctacct gccgcagttc
    15241 gggtcggagg tggcgcgcgg cgggcgctac gacggcatcg gcagcgcctt cggccgcagc
    15301 cggccggcga ccggtttctc ggccgacctg aaggccctgc tcgaactcgg cgagggcgag
    15361 ctgccggccg ttgcggcgat ctgggcgccg gccgaggacg atgccgcact cgaagcccgc
    15421 atcgcggcac tgcgaaacgc cggggagcgc gtgatccacg cgctgcccgg agccgaggac
    15481 ggcggtccgc gcgcgctcgg ctgcgaccgc gtgctggtgc gccgcggcag cgactggcac
    15541 atcgaagaca tcggaccggt cgccggctga aggccgtcgc gtccgctcac ctccccccat
    15601 acctgcttga cggacctgaa acatgggcaa gaacgtcgtc gtgatcggca cccagtgggg
    15661 tgacgaaggc aagggcaaga tcgtcgacct gctgaccgag cgggccggca gcgtcgtgcg
    15721 cttccagggc ggccacaacg ccgggcacac gctcgtcgtc ggcggcagga agaccgtgct
    15781 gcacctgatc ccctccggca tcctgcgcgc cggcgtgcag tgcctgatcg gcaacggcgt
    15841 cgtgctcgcg cccgatgcgc tgctcgccga gatcgaccag ctcgaagccg ccggcatcga
    15901 cgtgagcggg cggctgaagc tctcggaagc ctgcacgctg atcctgccgt accacgtcgc
    15961 gctcgatcgc gcccgcgagc aggcacgcgg cacgcgcgcg atcggcacga ccgggcgcgg
    16021 catcggcccg gcctacgagg acaaggtcgc gcgccgcgcg ctgcgcctcg gcgatctctt
    16081 tcaccgcgag cgcttcgccg ccaagctcgg cgagattctc gacttccaca acttcgtgct
    16141 cgccaactac ttccacgccg agcgcgtcga cttccagaag acgctcgatg atgcgctgac
    16201 gatggccgag cgcctgaagc ccatggtcgc cgatgtcacg cagctgctcg ccgaggccgg
    16261 cgcgcgcggc gagaacgtgc tgttcgaagg tgcgcagggc gcgctgctcg atgtcgatca
    16321 cggcacctat ccgttcgtga cctcgtcgaa caccacggcc ggcggggccg cgaccggcac
    16381 cggcatgggg ccgcgctatc tcgactacgt gctcggcatc accaaggcct actcgacgcg
    16441 cgtcggcggc ggcccgttcc cgaccgaact cgatgacgag gtcggcgagc acctcggcac
    16501 caaaggccac gagttcggcg cgaccaccgg ccggcggcgg cgctgcggct ggttcgatgc
    16561 cgtggcgctg cgccgctcga tccagaacaa ctcggtctcg ggtctctgca tcaccaagct
    16621 cgacgtgctc gacgggctct cgacgctgaa gatctgcacc ggctacaacc tcgacggccg
    16681 catcgtcgac tacccgccgc tcggggccga ggccttcgag gtctgcgagc cggtctacga
    16741 ggagatgccg ggctggagcg aatcgacctt cggcatccag tcgctcgccg acctgccggc
    16801 cgcggcgcgc gcctacctga agcgcctgga ggcactcacc ggcgcgccca tcgcgatcgt
    16861 ctcgaccggg ccggaccgcg aggagaccat cgtgctgcag gaccccttcg cctgagcggg
    16921 gcctgcccgc ccgccgtgtc ttgcggagcc cggccgccgt cgccgggctc cgtcgtttcc
    16981 agcgccgtga gatcgcgccc atgcccgaag cccgccgcat gctgttcccg gtcagctggg
    17041 acgaactgca tcgcaacacc cgccagctcg cgtggcggct gctggagcgc gggccgttca
    17101 ccggcatcgt cgcgatcacg cgcggcgggc tgatcccggc cggcatcatc gcccgcgagc
    17161 tcgacatccg gctgatcgag accttctgca tcgccagcta cgaccacgcc cgccccgatg
    17221 ccgaggccgg cgaggcggtg ctgctgaagg gcttcacggc cgctgccggc ggcgccggct
    17281 ggctggtcat cgacgagctg gtcgacaccg gccgcacgat gcagctgctg cgccgccacg
    17341 ctccggaggc gctgtacgcg gcggtctacg ccaagccgct cggccggccg caggtcgacc
    17401 tgtacgtgac cgaggtctcg caggacacct ggatccagtt cccgtgggat accgagatcc
    17461 agtacgcgaa gccgctggtc gagcgctgaa ccccgttccg cccggccgcc gtcaagcccg
    17521 gctgccagcg cgcagcacgg cagcgtgact ttcgccggct gccgcgctct gatggcgccc
    17581 cctcgaccgg gccacgcacc gcaggcggtg ccggcacggt ttctgccgta cgcgcatccg
    17641 gcgggcgatc cccgcggctg cgcgtccttc cgaccccccg cacgatggag ggctgccccg
    17701 atgtccgtgt tcaccgatcc cgatttcgac ggccacgaac aggtcgtcca ctgccacgaa
    17761 cgcgcgagcg gcctgcacgc gatcatcgcc atccactcga cccggctcgg gccggcgctc
    17821 ggtggcatcc gcatgtatcc gtacgccgac gaggccgggg cgctgaacga tgcgctgcgc
    17881 ctggcgcgcg gcatgagcta caagtccgcg ctcgccggcc tgccgctcgg cggcggcaag
    17941 agcgtgatcc tcggcgaccc gcgccgcgac aagacgccgg ccctgctgca tgcgatgggc
    18001 gaggcgatcg agcgcctcgg cgggcgctac gtcggcgccg aggattccgg caccgcggtt
    18061 gccgacctgc agctcatggc gcagacgact gcgcacgtga ccggcctgca ggacaagccc
    18121 ggcctcgacg gcggcgtgcg ctccggcgat ccgtcgccgg cgaccgcgct cggcgtgttc
    18181 accggcatcc gcgccgccgt gcgccatcgc ttcgggcgcg aggatctcgc cgggctgcgc
    18241 gtcgcgatcc agggcgtcgg cagcgtcggc caccggctcg cgcagctgct gcatgccgcc
    18301 ggcgcgacgc tgttcgtcag cgatgtctgg gcgccgaacc tcgaacgcgc gctgcgcgag
    18361 ttcggcgccg agccggtcac acccgatcgc atccacgccg cgccggtcga cgtgtacgcg
    18421 ccgtgcgcgc tcggggccgt gctcgatgac gtgacgatcc cgcaactgtg cgccgcgatc
    18481 gtcgccggtg ccgccaacaa ccagctcgcg caggcgcgcc acggcgaggc gctgcgcgcg
    18541 cgcggcgtgc tgtacgcgcc ggactacgcg atcaacgccg gcggcatcat cgatgtctgc
    18601 gccgagcgcc tcggcgagga cgccgccggc gtgcgggcgc gcgtcgagcg catcggcgcg
    18661 acgctcacgg cgatcttcga gcaggccgaa agcgaagggc tgccgacgca gctcgtcgcc
    18721 gaccggctgg ccgagcggcg gctgtacggc tgatgccgct cccgcggacc ggtgtcgtgc
    18781 ttcgcactgc cccggcgcgc ctttggcggt ggcggcgggc tgacgggcga ccggtgtggc
    18841 cctcgtcgga cctggccccc gccggggccg gtccgcgcct gagcgggcgc ggcctcagtg
    18901 cggcgcggcg caggccagat aggcgaggcg cttgagctcc tgacgcccgc gcgtgacggt
    18961 gtcgaggatg aggccgcagg cgaggctcag gaagccgagc agcgcgagcc cggtcgccag
    19021 gatcgcggtc gggaagcgcg ggaccaggcc ggtcgtgagc caggtctgca cgatcggggc
    19081 ggcgagcgcc acggacagca gcagcagcag cgcgcagacc gccccgaaga agctcagcgg
    19141 ccgttccgac ttcatgagct tcaggatcgt catcaggatg cgccagccgt cccggtaggt
    19201 gctgagcttg ctcgtcgagc cggcgggccg gctgcggtac ggcgtgtcga actcgccgat
    19261 cggcatgcgc agttcgagcg cgtggacggt cagttcggtt tcgatctcga aacccgcggc
    19321 gagcgccggg aaggacttga cgaagcgtcg cgagaacacg cggtagcccg acaggatgtc
    19381 ggtgaagctg cgcccgaaca gcatggcgac gcagccggtc agcaggcggt tgccgaagcg
    19441 gtgtccggcg cggtaggccg agcgttcctc ggtgtgccgg caaccgacca ccatgtccgc
    19501 gtgctccccg atgagcttgc gcaccatcgc cgcggcgctg tcggcgtcat aggtgccgtc
    19561 gccgtcgacc atcacgtaga gatcggcttc gacgtctgca aacatgcgcc gcaccacgtt
    19621 gcccttgccc tgctggcgca ccgtgcgcac atcggcgccg accgagtgcg cgatggcggc
    19681 cgtgtcgtcg acggagttgt tgtcgaacac gtagatcgtg gcatcgggca gcgcgcggcg
    19741 aaagtcagtc acgacctcac cgatcgtgac ggcctcgttg tagcagggaa tcaggacggc
    19801 gatccggtaa tcggcttcgg aatgcacggg cagcgtcatg atcggggaaa ctctccggca
    19861 ggcgaacggt tctggggcgg tgccggcatc gcgcaattcg gcgccggcca ttggggaatc
    19921 gatgttcagg ggccgacggc atgcacgatg gcggccttgt acgcgcgcat gagcggctgg
    19981 tcgcgcgcgg tcgccgaata gcgtccggca tcgaaatggc tcgtcgtgat gcgcatgacc
    20041 accggctgcg tgacgatgct tgcgatcagg gccggcagtg cgggagacat ccgctccagt
    20101 tgcatctggg tttccttgct cgcgtggctc tcgggcagca gcatcggcac gatcatgtaa
    20161 tacccaggcg ccagcgacgg catttcgtcg aacacaacga gttgccggtg cagttcgggc
    20221 ggcagcatgt ggcgcaggtg atggtgcagc acactgtcca cgtggatcgg gcggccgttc
    20281 gcggacagga tgcggatggc gtcggccgca ttgcggtaat agtcggcgaa ggtgatcgac
    20341 ccggcctcgg ctgccgcgga cttcacgccg gtcgcaagca gcgcgatcag caggggtatg
    20401 gtcgctgcgc cggtgatgtc gagccggccc ggccagcgtc ggccgacggc atcaagcgcc
    20461 agaccgagag cgatggctgc gaacggaatg cacggcagca ggtagcggtc ctggatcggc
    20521 aggtggacca ggcgcgtgag gctcgacgag ccgacgatca ggtacagcgt cacgaataag
    20581 ccggtcagca cgaaaccggc gagaggccgg ttccagagca gcacgccgag tgcgccgagc
    20641 gcgccgagga ccagcatcgg accgttgcgc gcccagtcca cgagcagcgt cagcagtgcc
    20701 cagtatccgg ccttggcgaa ttcgagcgct ccggcggacg cctcgatggt ccggttgtgg
    20761 acctgggtca gtgcgtggct gcgatagaag aagtcgtcgg cgacccacca gtacgctgca
    20821 ccctcggcga ggtagaccgc gagcatcgcg agtccgtaca gcgcggcagc ctgcagccgg
    20881 cgcagcgggc cgacgtcacg catgccgatc aggatccaca gcacgagcgg cggcagcatc
    20941 agcagcgccg tgtccttcga ggaataggcg agtccggtca gcacgccggc gccgatcagc
    21001 cacacgccgc gtcgccgcgg ggtttcgatc gcgtggaaga tggccatcgc cgcgcccggc
    21061 atcagtacgg cggtgagatt gtccggtacg aacacgctcg catacaggat gctggtgccc
    21121 gagagcatga ccatgccgaa ggccacgagc gccgcgttca ttgaggcgtg gcggcgcatg
    21181 aatgcgtaga cgagcaggcc cccggcgatc gacaccgcaa tcgacagcat gcagccggta
    21241 tacaggctgt ggctcagcac ggtcggaatg ccgagcagca gcatgaacag catccgggaa
    21301 gcatgatggt gggcggaccc ttcgagtcgg ccgttcagca gctgttcgga atacatataa
    21361 tagttggaat cgtcagaacc gatcgggccg gcaaagaaga tcagccgcag ggccagcccg
    21421 ccgatcagca aactccagaa gattgccgcg gtccgtcgct ggctgtcctg actcgacatc
    21481 gtcgcctccg aaactgctgt tcttcggcga gtatgtcatc gggcggcctc gaacagtatg
    21541 gccttgccgc ccgctgcggc ttccgtggtc cgcggcagga ctggtgcagg acaaacacat
    21601 cttcttgcaa tgcgaaacag ccgctgagag gtaggcgttt ctggtgaagt ctggtcggaa
    21661 tccagacctc gccggaccga agttttcaga aacttacagg gaagctgcta tcacgcttct
    21721 gcttgacttt tcgcatgacg agaagatgcg tttgccttgg ggccggcgcg atttccgcaa
    21781 aaggtttccg cacaatgagg aatctccgcc gggaattcgg aaaatttttc ccggcggaga
    21841 cgattttttt caaacatttt cctccctgcc cccgcgggtc ggtgccggca atgcatgaaa
    21901 gggattttcc gctgacgaca agatgcgccg gcgcggcggt ccgcggcaca ccgccgccac
    21961 tgcgccggcc acgaggaggt tcagcggtag tagtaatacg tcgccttgta cggcacgcgc
    22021 gcgatctggc cggcctgcct gcgcgtacac ggcgcgttcg ggaacaggcc gccttcggtg
    22081 tcgacgcgct gcacgctctt cgtgcggccg aacacgccat ccccgctgtt gtccttcgtg
    22141 ccgagcagca gccacggcac ggccttgtcc tgttcgaccg gcttgcgggc ctgcggcacg
    22201 ccggtgaagc ggctgccgtc ggcggccgtc cacagcggac cggcgccgat cctgccgacc
    22261 ggcttgccgt cggcgtcgct cagcgtcgcc tcggtggcct tgaactgcca ggcgtggccc
    22321 ttcggggcgc ccggggtcgc gcggcattcg tacaggtaat cgccgctgcc gcgggtcatc
    22381 aggtaggcac tctggtcggc cggcggcttc aggtagtccg gggtttcggg cggctggacg
    22441 ctcgcgcagc cggcggcggc gagggcggcg gacagcgcga ggctgcgcac gatgcaggcg
    22501 gatcggttca tgtcgttatc tccgggtggt gatgcggcgc gacgacggcg cgcggcgaac
    22561 cggaatgcgc aggccgtcgt cgccggcaac tgtagaagcg cgcccgcacg ccggtgatta
    22621 ccggccgttg aatggcgggc aatgacgtgc aatgacgttg aatgacaccg ggcggcgggc
    22681 cagggaaccg ggcgctgcgg gcagggttca ctacgctggc tgtgaacctt gcgctgtcgc
    22741 cctgtccttc agggccttcg ggaatgtccg cgatgaccgt atccctgtcc cgcctgctga
    22801 gttccgatga tccgctgccg gtcagcgtgc tgaatcccgg cgcgcactcg ccgctgctgc
    22861 tgctctgtga tcacgccagc cacgccgtgc cggctgcgct tgccggactc ggcctgccgg
    22921 cggcggaact gacgcgccac atcggctggg accccggtgc ggcgctggtc agcgcgcatc
    22981 tggccgggcg cttcgatgcc gcggcggtgc tgtcgggctt ttcgcggctg gtcatcgact
    23041 gcaaccgcgc gccgggcgac ccgacctcga tcccggaaat cagcgacggc accgtcgtgc
    23101 ccggcaaccg ggggctggac gaggcggcgg tgctcgcgcg gctcgatgcc tgcttctggc
    23161 cgtatcacca ggtcatcacc gcgcgcatcg cgcagatgcg cagcgacggc cgcgtgccgg
    23221 tcgtcgtcgc cgtgcacagc ttcacgccct gcctgaactg cgccggcggc gcgcggccct
    23281 ggcagatcgg cgtgctgtgg aaccacgacc cgcgtctggc cgtgccgctg atcgagaccc
    23341 tgcgcgcgca gggcgtctgc gtcggtgaca acgaaccgta ttcggggcgc gattcgagct
    23401 acacggtcga gacccacgcc gccgccgccg gcctgccgca cgtgctgatc gagctgcgtc
    23461 aggacctgct cggcgatccg gccggctgtg cgcactgggc cgacgtgctc ggcgatgcgc
    23521 tggcgccgct gctcgcgcgt ccggaactcg cgcgcgcgga gttccactga tgccgggcgg
    23581 cccgcaaccg ccgttcacgc tcggcatcga ggaggaatac ttcctcgtcg agcgcgccag
    23641 ccgcgatctg gcgcgcgagc tgccgccggg cctgctcgat gcctgcaacc gccgtctcgg
    23701 cgaccgcgtg acgccggagt tcctgcgcgg ccagatcgag gtcggcacgc gcacctgcac
    23761 gagcctcgcc gaggcgcgcg ccgatctggc ggagctgcgc acgacgctcg ccgaggttgc
    23821 cgcgcagcac ggctgcgccc cgatcgcggc ttccacgcac ccgttcgcgc gctggcacga
    23881 tcagcagccg accgaccgcg cgcgctatcg cgccatcgcc gaggacctgc agggtgtcgg
    23941 ccggcggctg ctgatctgcg gcatgcacgt gcacgtcggc ctgcccgacg atgacctgcg
    24001 catcgacctg atgggccagt tcgcgtactt cctgccgcat ctgctcgcgc tcagcacctc
    24061 atcgccgttc tggcgcggcg aggacaccgg gctctgttcg taccgcgtca gcgtcttcaa
    24121 cgagatgccg cgcaccggcc tgccgccggc gttcgcgagc tggggcgagt acgcgcgcca
    24181 cgtgcgcgtg ctggtcggtg ccggcctgat cgaggatgcg accaagctct ggtgggacct
    24241 gcggccctcg gcgcgcttcc cgacgctgga gatgcgcatc accgatgtct gcccgcgcct
    24301 cgacgatgcg ctggcgctgg cggcactgac ccgctgcctg ctgcgccggc tctggcggct
    24361 gcggcgcgac aaccagcgct ggcgcgtcta caagcgcatg ctgatcgacg agaaccgctg
    24421 gcgcgcgcag cgctacggcc tcgccgaagg gctggtcgac ttcgggcgcg gctgcatcgt
    24481 gccgttcccg gagctgctcg ccgagctgca ggcgctcgtt gccgaggatg ccgcgcactt
    24541 cggctgcgag gccgagatcc gtcacctggg caccatcctc gaacgcggca ccggcgccga
    24601 ccgccagcgc gcggtgcggc tcgcggcgct ggagcgcggc gccgcgccgc tggcggcgat
    24661 gcaggccgtc gtcgatgacc tgatcgtgca gacgcaggtc accggctgat gcgcgatcgg
    24721 catggcagcg gacaaaacag gtacagtgcg cgcctttgac cgccgctgcc cgatccccgc
    24781 ctcatccatg ccgatccgct ccgaaaccct gctcccgccc ggctgcctgc gccgcaccac
    24841 cgacgccggc acgctcggcc tgtccacgac cgccggcctg gagccgctgc cgctgctgtt
    24901 cgggcaggag cgggcgctgg cggcgatcga cttcggcgtg cgcatgggcg gcgacggcta
    24961 caacctgttc ctgctcggcc ccggcggcat cggcaagcgc cggctggcgc gccgggtgct
    25021 gaacgcgcgt gccgatgccg aggccacgcc cggcgactgg gtgtacgtgc acaacttctc
    25081 cgaaccgctg aaaccctgcg ccctcggtct ggcgccgggg caggggccgg cgtttcgcga
    25141 ggcgctgaag acgctggtcg gcgagctggc cgacctgctg cccgatctct acgagggcga
    25201 ggactgccgc gcccgccgcc aggcgctgaa ggccgagttc gtcgcccgcg acgaggagcg
    25261 cgtcaagagc ctgcgcgagc gcgcgcgcgc gcaggacgtg gcgctgacgc agaccccgga
    25321 cggcttctcg ctggtgccgc tcgccgatgg cgagccgatg ccggacgagg actacgaggc
    25381 gctggccgag gacgcgcgcg ccgcggtcga tgcggcgatg tcggaggtgc gcggcgcgct
    25441 tgaggacctg ctcgacaccg cgccgcgcga gcagcgcgcc taccgcgagc gccagcgcgc
    25501 gctgcgtcag gcgctgatgc gccgcgaggt cgagccgcgc atcgcgcagc tgcgcgaggc
    25561 ctggagcgga ttgccagcgg tgctggcgca cctcgatgcg ctggctgccg acctcatcga
    25621 gcacgtcgat gacttcctgc ccgccgatga cgaggacgag gagcgtccgc cggcgcgcca
    25681 gctcaagcgc taccgcgtca acgtcgtggt cacgcgcacg cccggtgccg gcgcgccggt
    25741 cgtgtacgag gaccatccgc tgcaccagaa cctgctcggg cggctcgaat acgcagccga
    25801 gagcggcaac ctgctcaccg acttcacgct gatccgcgcc ggctcgctgc acacggccaa
    25861 cggcggctac ctgatcctcg aagcccggca gctgctgtcg cagcccttcg cgtgggaagg
    25921 gctcaagcgc gcgctgctgt cgcgggcgat ccgcatcgtc tcgcccggcg agctctacgg
    25981 gcaggtgacg acggtgtcgc tcgaccccga gccgatcccg ctcgcggtca aggtgctgct
    26041 gctcggcgat gccgaggtct acgagctgct gtcgcagctc gatccggact ttccggcgct
    26101 gttcaaggtc gtcgccgacc tcgaacccga cgtgccgcgc gatgccgcca gcgagcgcct
    26161 gtacgcgcgc ctgatcgcga ccatcgtcca cgaggccggg ctgcggccgt tcgcggccga
    26221 tgcgctcgcg cgcgtgatcg atgaggccgc acgccaggcc gaggacgccg cacgcctgtc
    26281 gctgcatctg gccgggctga gcgacctgct gcgcgaggcc gacttcgtcg ccgccgagac
    26341 cgggaccgat accgtcacgg ccgcgcaggt cgccgaggcg ctggcgcggc gccggcgccg
    26401 gcgcggcggc atcgaggcgc gcatgctgga ggcgatccgc cgcggcacgg tgctgatcga
    26461 cacgcacgga gccgcgatcg gtcaggtcaa cggtctggcc gtgctcgatc tcggcgacgt
    26521 gcgcttcgcc aagcccgtgc gcctgtcggc aaccgtgcgc ctcggcagcg gcgaggtcgt
    26581 cgacatcgag cgcgaatccg aactcggcgg cgcgatccac gccaagggtg tgctgatcct
    26641 caccagcctg ctcggcgcgc gctacctgcc cgatcacccg ctgtcgtttc gcgcgagcct
    26701 ggtgttcgaa cagtcctacg gcccggtcga cggcgacagc gcctcggtcg ccgagtgctg
    26761 cgcgctgctg tcggcgctgg cgaaactgcc gctcgcgcag cacctcgcga tcaccggctc
    26821 gctgaaccag tacggccgcg tgcaggcgat cggcggcgtc aacgagaaga tcgagggctt
    26881 ctgggccgtg tgccgcgatg ccggcctgac cggcacgcag ggcgtgatcg tgccggcggc
    26941 caacgtccag cacctgatgc tcgatccgga ggtggtcgag gcggtggcgg ccgggcgctt
    27001 ccacatccac gcggtcgccg agctcgacga ggcgctggcg ctgctgaccg gcgtggcggc
    27061 cggcgagcgc gacgcgcagg gccgctttcc gccggcctcg gtcaacggcc gcgtcgaggc
    27121 gcggctcgaa gccttcgcgc tggccgcgca gcgcagtggc ggcgcgcgtg actgagagac
    27181 cgggcctgtc cggacccctg cgccgggtcc ggacgcacgg caacgcgggc cggctccggg
    27241 ccgcaccggt cacggctggc cggggcgctg cggtccggag gcggtgatgc gcccgcgcac
    27301 cctgatccgt catccggcgc tgctgccgct gctggtgttc gcggcgctcg ccgcctgcat
    27361 cctcggcacc ggcatcggcc tgtaccgctg gcagcacgcg cagaccgagg acgaggcgca
    27421 gcgcatgctg ctcgcggtcg ccgacctgaa gagccgccag atcggcgact ggctgttcga
    27481 gcggcgcgcc gatgccgcga gcctcgccgc caaccgtctg gtcgacacca tgatcggcga
    27541 ctggctggcc gggggcacac cgcaggatgc ccggcacgcg cagctgctcg cccggctgcg
    27601 ctcgttccgc gacctgatgg gctatgccaa cgtcctgctg accgacccgc aggggcgcat
    27661 cgtgctggcc gcggaaaact ggccgcagga cgaaagcggc tggctgcgcg agcgcgtgcc
    27721 gccgctgctc gcgcagggcc gcacgatggt gtccgacctg caccggctcg acgccgccga
    27781 ccggccgctg ctgatggagg tggtgacacc ggtgctcgcc gggggcgggg aggcgcagcg
    27841 ggtcgtcgcc ggcctggtgc tgctgatcga tccgcgcagc tacctgtatc cggtcgtgca
    27901 gggctggccg accgcgagcg cgagtgccga agcgctgctg gtgcgcgtcg agggcgacga
    27961 catcgtgtat ctgtcgccgc tgcgccacga cagcgtcgca ccgctgggcc tgcgccggcc
    28021 gctggacgaa tccggcctgg ttgcagcgca ggcggcgcgg gggctgcgcg gcatcacccg
    28081 cggccacgac taccacgatg cgccggtgct cgccgcggtg cgggcgatcg aggacacgcc
    28141 gtggctgctg atcgtgcaga tcggcgccga ggaggtgttc gcgccgctgc gtcgcgccgc
    28201 ctggctggtc gggctgaccc tcgggctgct gctcgccgcc gccgccgccg tcaccagcgt
    28261 ctggatgtgg tggctgcgcc tgcgcgagcg cctgcatgcc gccgggctgg aggccgaggg
    28321 catgcgcctg cgcagcgaac tcgaattcct gtccaaccac gccaacgaca tcatcctgct
    28381 cgccgatgac gagggccatg tgctgcgcgc caacgaccgt gcggtcgagg cctacggcta
    28441 ttcgcacgac gagctgaccg gcctgagcct cgcggcgctg cgcgcgcagg aggccccggc
    28501 cgaggcccag acgctgctgc ccggcagcgg cacggtctac gagaccgtgc accggcgtcg
    28561 cgacggcagc tgctttgcag tcgaggtcag cacgcgcagc ttccgcgtcg cggccacgcg
    28621 ctaccgccag tcgatcatcc gcgacatcag cgagcgcaag gtcgccgagg cgctgctgca
    28681 gcgccagacg cagctctatg cgctgctcac cgccaccaac gaggccatcg tgcgcgccgg
    28741 cgacgtgcgc gcgctgtacc gcgaggtctg cgccgcggcc tgtcgcagcg gcggcttcgt
    28801 gaccgcgtgg atcggcacga tcggcagcga cggcaaccgc atcgaggttg cggccagcgc
    28861 cggttcggcc gaggtcgtcg cctacgtcga ccggctcggg ctggtgctcg atgccagccg
    28921 ccccgaaggt cgcggaccga ccgcgaccgc gatccgcgag gggtgttcga tgctgtcgaa
    28981 caccatcggc gacgactcgc cgatggcgtt ctggcagcag gcggccgagc acttcggcat
    29041 cggttcggcg gcctgctttc cgctgcgctg cgccggccac acggtggccg gcatcagcct
    29101 gtacgcgccg gtgccggggt atttccgcga cgatctgctg cacctgctct cgcgccttcg
    29161 cgttgcgctc cgctttctcc a
//
LOCUS       DAOQIE010000009        28963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_684_length_28963_cov_7.875294, whole genome shotgun sequence.
ACCESSION   DAOQIE010000009 DAOQIE010000000
VERSION     DAOQIE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 28963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28963
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_684_length_28963_cov_7.875294"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PK144_01135"
     CDS             complement(<1..281)
                     /locus_tag="PK144_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF64354.1"
                     /translation="MHLRELVGYCDTLLAAASFSDYCPNGLQVEGRPDVRLLVGGVTA
                     CQALLEAALDLGADAILVHHGYFWRGEDARVTGQKRRRLATLLRHDLSL"
     gene            complement(293..1351)
                     /gene="hisC"
                     /locus_tag="PK144_01140"
     CDS             complement(293..1351)
                     /gene="hisC"
                     /locus_tag="PK144_01140"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNF64355.1"
                     /translation="MSRYWSEVVGRLTPYVPGEQPQIPNLVKLNTNEHPDGPSPRVLA
                     AIRAALDEGLRLYPDPNAGRLKQALAARHGLTAAHVFVGNGSDEVLAHAFHGLLRHER
                     PLLFPDVTYSFYPVWCGLYDIGFETIPLAGDFSLRVEDYRRPNGGIVFANPNAPTGRA
                     LALADIERLLGFNRDSVVLVDEAYVDFGADSAAALVAQYPNLLVVQTLSKSRALAGLR
                     VGWAYGDPALIEALERIKNSFNSYPLDRLALAGAVAALEDDEYFDVHRRAVIDSREQL
                     AQALTDHGFEVLPSAANFVFARHPAHDAAGLAAGLRAHGVIVRHFRLPRIEQFLRITV
                     GSPAQNAALLAALAKLHG"
     gene            complement(1348..2670)
                     /gene="hisD"
                     /locus_tag="PK144_01145"
     CDS             complement(1348..2670)
                     /gene="hisD"
                     /locus_tag="PK144_01145"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNF64356.1"
                     /translation="MDIARLNYSDPDFWPRLDRLTAWEGVSDAHVAGTVAQIIADLRA
                     RGDAALLELTNRYDRTAFAAAAELEIPAERLDAALCALPPAQRTALETAAARVRAYAE
                     RQKLDSWSYTEADGTLLGQQVTALDAVGLYVPGGKAAYPSSVLMNAIPAKVAGVRQLV
                     MVVPTPDGEQNDLVLAAARLAGVDRVFAIGGAQAVAALAYGTASVPRVDKIVGPGNIW
                     VATAKRAVYGQVGIDMIAGPSEILVICDGDTDADWIAMDLFSQAEHDEDAQPILISPD
                     AAFLDRVAAAIERLLPTLARADIVRVSLASRAALIRVADLAEAAEVANYIAPEHLELS
                     VADPQALLPSIRHAGAIFLGRHTAEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQKR
                     SSLIFCSPDGAATLGRTASVLARGEGLSAHARSAEYRIPGFVPPEAAS"
     gene            complement(2745..3386)
                     /gene="hisG"
                     /locus_tag="PK144_01150"
     CDS             complement(2745..3386)
                     /gene="hisG"
                     /locus_tag="PK144_01150"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNF64357.1"
                     /translation="MSDTLTIALSKGRIFQETLPLLAAAGIRPLEDPETSRKLILDTD
                     RPDVKLVIIRATDVPTYVQYGAADLGVAGKDVLMEHGGEGLYEPLDLGIARCRLMTAG
                     RPGVPEPRGRMRVATKFANVARRFYAQQGRQVEIIKLYGSMELAPLVGLADRIVDIVD
                     TGKTLRANGLEPLELIADISSRLVVNRAAMKMKHARVRAILEHMAAATGRAVA"
     gene            complement(3413..4669)
                     /gene="murA"
                     /locus_tag="PK144_01155"
     CDS             complement(3413..4669)
                     /gene="murA"
                     /locus_tag="PK144_01155"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNF64358.1"
                     /translation="MSKLIITGGETLRGEVRACGAKNAVLPILAATLLADGPVTIGNV
                     PHLQDVTTTMELLGRLGAQLVVDEKLNIEVDPRCITSHTAPYELVRQMRASILVLGPL
                     LARFGEAEVSLPGGCAIGSRPVNLHIKGLAAMGADIRVEEGYIRARCPRLKGARIVLD
                     LVTVTGTENLMMAAALAQGTTILENAAREPEVVDLANCLNAMGAQVHGAGTDTLVIDG
                     VERLHGAHYRVLPDRIETGTYLAAAAITGGCVRVKDTRPDTLEAVLVKFEEAGAVITT
                     GPDWIELDMQGRRPKAVHIRTAPYPAFPTDMQAQFCALNAVAEGVGTITETVFENRFM
                     HINELSRMGAQFQLEGNTVISTGVAQLTAAPIMATDLRASASLVLAALVGKGDTVVDR
                     VYHIDRGYDCIEEKLGSLGALIRRVP"
     gene            complement(4683..4934)
                     /locus_tag="PK144_01160"
     CDS             complement(4683..4934)
                     /locus_tag="PK144_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNF64359.1"
                     /translation="MQAADIQRLIQEALPSAEVLVRGDDGVHFEAVVISAEFAGKSLI
                     AQHRLVNAALGDRLQREEIHALGLRTFTPEAWAAQQAQA"
     gene            complement(5029..5349)
                     /locus_tag="PK144_01165"
     CDS             complement(5029..5349)
                     /locus_tag="PK144_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF64360.1"
                     /translation="MSTGAAAVQPATLTRLGPGRLALGGDLVIDAVAALWDAAAGELA
                     GGETVTLSLDGVRRADSAGVALLVEWLRAARAGGGELRLSGLPAQMRAILAVSDLDTL
                     FPDA"
     gene            complement(5351..5986)
                     /locus_tag="PK144_01170"
     CDS             complement(5351..5986)
                     /locus_tag="PK144_01170"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF64361.1"
                     /translation="MHSILHRLLKPLCALLLGIGLFAAAGPGTAADTPAAQAVEDTAS
                     RMLVALKQQREALRADPSKIYGLVERIAVPRFDFEAISQWVLGRNWRGASPEQRQRFT
                     AEFQNLLVRIYSNALLEYSDETVRFLPGAAPNADGDMLVRSEFVRRAGPVVPINYSVH
                     QKNGQWLIYDVTVDGVSLVANYRTTFANQVRDQGMDGLIASLAKLNASGGR"
     gene            complement(6046..6555)
                     /gene="mlaD"
                     /locus_tag="PK144_01175"
     CDS             complement(6046..6555)
                     /gene="mlaD"
                     /locus_tag="PK144_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNF64362.1"
                     /translation="MNPSRTVEVLVGAFVMLGLAALVFLAMQVSGLTSLGADAAYRVH
                     ARFENVGGLKVRSPVSMAGVRIGRVTAIDIDPQTFQAVVELSISARHANLPADTSAKI
                     LTSGLLGEQFVGLEAGGADAVLKDGSTIKLTQSALVLENLISQFLFSKASEGRPETPA
                     PAPGPAGQP"
     gene            complement(6556..7335)
                     /gene="mlaE"
                     /locus_tag="PK144_01180"
     CDS             complement(6556..7335)
                     /gene="mlaE"
                     /locus_tag="PK144_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNF64363.1"
                     /translation="MIAALGRWALTVFARTGRASLMLLAILAGLPSVLPRPRLLVRQL
                     YSVGVLSLPIIVVAGLFVGMVLALQGYTTLADFGAAGSLGTVVALSLVRELGPVVAAL
                     LYAGRAGSALTAEIALMKATEQLSGMEMMAVDPVRRVLAPRFLAGILAVPLLAAIFTA
                     VGILGGYLVGVGLLGVDDGAWWAQMQAKVQPLRDVVNGVVLKSFVFGVVANWIAVFEG
                     HDAQPTSEGIGRATTRSVVNTSLAVLGLDFVLTALLFGGVR"
     gene            complement(7332..8171)
                     /locus_tag="PK144_01185"
     CDS             complement(7332..8171)
                     /locus_tag="PK144_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF64364.1"
                     /translation="MPDVPDSPAAADSLVRIRGLRFLRGTRVIFDGVDLDIPRGRVTA
                     VMGPSGTGKTTLLRLIGGQLRPAAGSVVLDGHEVPALRRRELYALRRRIGMLFQSGAL
                     FTDLSVFENVAFPLREHYPRLPARLLRTLVLMKLEAVGLRGARDLKPAELSGGMARRV
                     ALARAIALDPMLMLYDEPFTGQDPISMGVLVQLIRTLNDALGMTSVVVSHDVAEVCSI
                     ADYVYLISGGRVVASGTPAELRASGSDWVGQFMHGLPDGPVPFHYPARDYASELLDGG
                     GRS"
     gene            8381..9352
                     /locus_tag="PK144_01190"
     CDS             8381..9352
                     /locus_tag="PK144_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNF64365.1"
                     /translation="MNPDRLRDLGRTALRIEAQAIEHLIERVDAAFVAACECLLACRG
                     RIVVLGIGKSGHIGGKLAATFASTGSPAFFVHPAEASHGDLGMITAQDVVLALSNSGT
                     TDELLMLLPLIKRLGVPLVALTGNPESPLARAADVHLDVSVAQEACPLNLAPTASTTA
                     ALAMGDALAVSLLEARGFTADDFARSHPGGRLGRRLLLLIDELMHTGERLPRSRPDDP
                     LPVALLEMTRKGLGMTIATDDDDRMLGVFTDGDLRRALDRRIDLHAATLAEVMTRPGF
                     SVRTGLLAAEALNLMERHRINALPVLDRDDRLAGALNMHDLLRAGVL"
     gene            9369..9947
                     /gene="lptC"
                     /locus_tag="PK144_01195"
     CDS             9369..9947
                     /gene="lptC"
                     /locus_tag="PK144_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNF64366.1"
                     /translation="MRTLLVLVALALLAGLSGWFLNDLERDLREVRARDDRLPLTIAE
                     NFVATVMNARGERLYVLTGPHLEQFPAPRGTFVTRPLAHTYENLAPVWEIRAEQGWIA
                     PGTETIELRGDVHAERPASTGYVPVIMTGSNLRLIPDKNYAESAEPVRVQTPDGVIDA
                     VGFHAWLDEDRIELLADVRGHYAPPAQKPDRP"
     gene            9913..10500
                     /gene="lptA"
                     /locus_tag="PK144_01200"
     CDS             9913..10500
                     /gene="lptA"
                     /locus_tag="PK144_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNF64367.1"
                     /translation="MRRLPRNLIAPSRITRGRITSGLIAPGLLACALSAQALPEDRQQ
                     PVNLVADRAEMNQQTGVATYEGNVIVTQGTMRLTGDKVVVYTQEGELQRMESFGKPTT
                     FKQKPAADKEEILGESLKLEYDARTGIVIATGKAKITQVKDTFTGDRIEYDVNKDLVK
                     ARGGESAGRVQIILQPKPEGGTPVPGARPAQPKKP"
     gene            10504..11229
                     /gene="lptB"
                     /locus_tag="PK144_01205"
     CDS             10504..11229
                     /gene="lptB"
                     /locus_tag="PK144_01205"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005281703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNF64368.1"
                     /translation="MARLEAREIAKRYKGRRVVDAASVHVDSGEVVGLLGPNGAGKTT
                     SFYMIVGLIGCDEGHVLLDSREITQLPMHARARLGVGYLPQEASVFRKMTVLDNVLAI
                     LETRGDLDRSARTERAETLLHELNVGHLRDNLGISLSGGERRRVEIARALAAEPRFML
                     LDEPFAGVDPLSVHDIQRIVRHLCDRDIGVLITDHNVRETLGICDRAYILAEGRVIAT
                     GTPAEILADRRVREVYLGEHFRL"
     gene            11321..12784
                     /locus_tag="PK144_01210"
     CDS             11321..12784
                     /locus_tag="PK144_01210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNF64369.1"
                     /translation="MRQSLQLRLGQHLTMTPQLQQAIRLLQLSSLDLQAEIQTVLDSN
                     LMLERSDDTPDPEVPTAEAALSETVPPAQDRTETPTDTIPNELPVDTEWGDVYDSYDG
                     STSYSRGDDDDNRDLFENHGNDQPSLQEHLLWQMRLTPFSALDAEIAEALIDATDASG
                     YLTLTLDDILEGLRRTHPDLERDEVEAVLHRVQHFDPPGICARNPSECLMLQLDTLPG
                     DTPWRREAIELVRQYLELLAGRDFNLLMRRMKLTREQLQEVIALIQTLNPHPGTALSA
                     ETAAYVVPDVFVFRGRDGWRVDLNPDSAPKIRINAEYARLVRRADSSADNQYLKNHLQ
                     EARWFLKSLRNRHDTLLKVARCIVDRQISFFEKGPEHMRPLVLRDIAEAVEMHESTIS
                     RVTTQKYMHTPRGIFEFKYFFSSHVATSDGGEASSIAIQAMIRKLIAAENTQKPHSDS
                     RLAELLAAEGIQVARRTVAKYREAMSIPSSTDRKRIG"
     gene            12885..13205
                     /gene="raiA"
                     /locus_tag="PK144_01215"
     CDS             12885..13205
                     /gene="raiA"
                     /locus_tag="PK144_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNF64370.1"
                     /translation="MQINLTGHHVEISQPLRDYVQDKIGKIERHCDSITSAYVILSVE
                     KLRQKAEATLHVAGNDIHADAIEEDMYAAIDVLADKIDRQIKKHKEKLTDHHRSDKAT
                     GTPS"
     gene            13244..13717
                     /gene="ptsN"
                     /locus_tag="PK144_01220"
     CDS             13244..13717
                     /gene="ptsN"
                     /locus_tag="PK144_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HNF64371.1"
                     /translation="MNISDLLTAGRVACRSDVVSKKRVLELVSELIAAGASGVDARAI
                     FDSLIGRERLGSTGLGNGVALPHGRLAGTEQALGAFVRLGKGVEFDAIDGQPVDLVFA
                     LLVPEHFTDEHLKILAHLAEMFSDRAFCERLRTTSADADLHALLTGWSPSADAAL"
     gene            13720..14667
                     /gene="hprK"
                     /locus_tag="PK144_01225"
     CDS             13720..14667
                     /gene="hprK"
                     /locus_tag="PK144_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNF64372.1"
                     /translation="MIAALPADQAFAALRAALALQWIGGRSAGARLICREGESLPPRS
                     LGGLLNLVTAPRVQVLGREELAFFASLDAGARASLLDRLLATPVGATVLCGDSPLIEE
                     FSSYADRHESPLWQTSSTPGEVLDRLSEYQHELDASSTVLHGVFLEVYGLGVMLSGES
                     STGKSELALELLSRGHRLIADDAPKLTRVAPQVIEGTCPDALRNFLEVRGLGILNVRR
                     MFGDSAIKRNKRLRLIVHLVRLEETSLPAEVRLSGARATRYILDVAIPEITLPIAPGR
                     NLAVLVECAVRDHILRLGGYNAERDLMERLQDEMTGGAA"
     gene            14664..15524
                     /gene="rapZ"
                     /locus_tag="PK144_01230"
     CDS             14664..15524
                     /gene="rapZ"
                     /locus_tag="PK144_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNF64373.1"
                     /translation="MRLIVVSGLSGSGKSVALATLEDCNDYCVDNLPLGLLPAFAEEL
                     LTHQRPSLETAAVGIDARNFRDQLERFPDIVAQLRAARFEVDIVFLQADAETLLKRFS
                     DTRRPHPLSVEHGTPLLEAIEAERRLLAPIAAHATLVIDTSHTNLHELRELLRTRLHG
                     GEIGQLSLVFESFGFKNGLPDDADYVFDVRFLPNPHWQSALRPLTGRDEAVARFLEAH
                     EVVESAVADLIRFLETWLPAFETGSRSYLTVAVGCTGGQHRSVYVTERLARHFGATRR
                     AVSVRHRELS"
     gene            15521..15925
                     /locus_tag="PK144_01235"
     CDS             15521..15925
                     /locus_tag="PK144_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNF64374.1"
                     /translation="MSVGLLLITHNRLGDELLATATTTFAHCPMRAATVRVGERCDPE
                     ALVAEARGKRAEVDDGSGVLVLTDIYGSTPSNVAHRLVDDGGVRVIAGVSLPMVIRVM
                     NYCRLDLLALAHKAVSAGRDGIVLTPEDDAAV"
     gene            15963..16232
                     /locus_tag="PK144_01240"
     CDS             15963..16232
                     /locus_tag="PK144_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNF64375.1"
                     /translation="MIQRDLTIVNKLGLHARAAAKFVTTASSYQAAIQVTKGGRIVNG
                     KSIMGVMMLAAGKGSVITLSADGPDAEAALDGLATLVADRFGEGE"
     gene            16243..17976
                     /gene="ptsP"
                     /locus_tag="PK144_01245"
     CDS             16243..17976
                     /gene="ptsP"
                     /locus_tag="PK144_01245"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNF64376.1"
                     /translation="MPLALHGIGVSRGCAIGETYHLLRDQPEVLEYAIPQDMVDDEVR
                     RFEEAITEARRELEDIRSRVAPNVPAEVTSFIDAHLLMLEDPALADAPVRLIRQHSCN
                     AEWALKMQRDLLVQVFDDMDDAYLRARRDDVDHVVRRVQGILLAEEPAPDLLASRLEG
                     RIIVADDLTPADTILMQHQGVAAFVTEYGGPLSHTAILARNLGIPAVVGARHARRLLG
                     DEETIIVDGQNGVVLAELDEPVLEHYRERQRREQRGRRELVKLRRLPAISRDQVTIGL
                     QANIELPEDALAVREIGADGIGLYRTEFLYMNRPDTPDEEEHLASYLHVIRTLGPEQP
                     ITIRTADLGADKQVDGGRRGPVATNPALGLRGIRLCLKDHEMFRAQLRAILRASAHGK
                     VRMMIPMLSNAYEITQVLHLLDDCRQELRARGQAFDEQLPVGGMVEVPAAAVCARLYA
                     HRLDFLSIGTNDLIQYTLAIDRIDDEVNYLYDPLHPAVLALIASTITAGQAAGIPVSM
                     CGEMAGDARYTRLLLGLGLTQFSMHPNNLLEVKRVIREAEVGALARETAAILAETSPE
                     VLSARLAAFVA"
     gene            17973..18749
                     /locus_tag="PK144_01250"
     CDS             17973..18749
                     /locus_tag="PK144_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF64377.1"
                     /translation="MNAVPESGRLDRGDGHQLAWVRTPGTTPGVMFLGGFQSDMTGTK
                     ALALEAHCRAAGRAFLRFDYYGHGASSGRFEDGTIGRWRADALAAFDALTAGPQLLVG
                     SSMGGWLATLLALDRAQRVAGLLTIAAAADFVIDLLPARLSPDQQQQLARDGRIELPS
                     MYSDTPYPICARLIEEGRDHRLLHAPVPYHGPVRLLHGSADADVPWQTSLRLLERLES
                     HDARLVLVRGADHRFSDAAGIALMLATLEELIGAAAAGPG"
     gene            complement(18802..21234)
                     /locus_tag="PK144_01255"
     CDS             complement(18802..21234)
                     /locus_tag="PK144_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64378.1"
                     /translation="MGVERAARDRTAVSAGVERERIQLVALARELLLRWGQAAAQACT
                     AEALNAVSEITGRLVEECLSLDERGLAVRVRTVDTLLRELVRSGERPDSIEQVRLADA
                     IRRLHDAIVGPEAEPVPVARKAGATGPARDETGDHRPRVYVFGDAPERMAGYTTRLGE
                     HGYKAETFDDLQALHLALSLQLPQAVLLLGDAHDPRAGARLVSELRLAGLHEDLPVVL
                     VAPQTDSRCRLQALAAGADACLVEPLGGARLARRLHALIGNRQPQPHRVLLLDADPVR
                     LERLAGMLRGSGCHVRTLDDPGLLLETAAEEQPDVLVFDAGIPEADAPTLMRLIRDEP
                     ALAQLPVLLLAAEEDRLPVLTGGGVEVLPKPTAPGLLVGAVRAHAAGQRRRHGSAIRD
                     FTHRDASSGLATVEAFRKLVETALARNDAGIGALLYVAVDNLLQGDMSELRRARWRAR
                     FADALDDQLLGSDIAGMNADGAFLVFARRVDLPKLESFARDVAYALGCVTLDELGHGN
                     RPSVYVGVARAESGMLEQSVEAGKAVCVALRREGGDRVRLHPVLAGGPGGEEQRDWMG
                     RISGAIKEGRLFLMYQPITSLAGDHGERYEVLLRMRDTEGRTLLPEQFLGAATRLGLD
                     RLLDRWVVSRALRGLEGRLKEQGDTCFFIKLSGASVADPGFAEWLEAMLAKVSVQPRS
                     CVFEISEATLLVVPRRTEQLLRRVRAAGYGTALEHFAAAEESLALLPNLPLDYVKLDR
                     SLTANLATNPGRQNWMRELVHRIRSTGPRIIAGYVEDAQSLGLLWQAEIELVQGNFVQ
                     QPDEWLHHGSMI"
     gene            21352..21846
                     /locus_tag="PK144_01260"
     CDS             21352..21846
                     /locus_tag="PK144_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNF64379.1"
                     /translation="MRVLTRLSLLCLAAVLARGAHAEEIGSVDTAFKLLGANHKIVVE
                     AFDDPKIDGIACHVAKAQTGGIKGSLGLAEDSSDASIACRQVGPVVFRESFRDGEEVF
                     TERRSILFKKLRVVRFLDRKRSTLVYLVYSDKLIDGSPKNSLSTVPVMPWPTGSVALP
                     PPAR"
     gene            complement(21859..24312)
                     /locus_tag="PK144_01265"
     CDS             complement(21859..24312)
                     /locus_tag="PK144_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64380.1"
                     /translation="MPLEPLPASREPGLVLRDEDRTLLSTLASDLRRHWQRARSSHFE
                     AAEMVDLRMFVRGLADTCAQAAGFALAGQAQALDTALREAGWSGKRPDPARAQALGER
                     VEALAAALSQSASTGRTPESASMTVAASAPASAPRVFLVDSEPAQAKFLSLQLAAQGY
                     AAESFVDWDALVHAMASRQPAALIADIDAPHGVFGGADQVSELRRAGKLLCPVLFLSA
                     RADSRTKLIALRAGGVGYFVKPVAAPELATRLGELISHQEPAPYRVVMVDDDRAFLEL
                     QSSRLQAAGFEVKALSNPLGVLEAAQGFEPDVFVLDLYMPEADGCELTRLIHEEPQFA
                     NTPVMLLSGESDPTAHARVIVAGAVDVIVKPADPVRFIERVRARAMQYRQNFSRARRP
                     DRADTVTGLLTMAQFTEALEARLAHPREAEGALSALLYVSLGGYLDWSKRLDAARLEQ
                     VRTRVARVISERQQGNDIVGWDNAGRVIISTRQPDLPNLQSYARALIDAIGGVELSDL
                     GPGPRLVPSIGVCRADAGPVHGLIGDAAQLAAQQAREAGAGVRLQPLLVSSSAPDLEE
                     QRRWHSIINDAIREHRLFLVYQPITNLLVADRSEWNEVLLRMRDASGQTLLPGQFLPV
                     AAKFGLDRLLDRWVVSRALRGLDARQQDHPGTVFFVKLGTASVADPSFPEWLRQMLGK
                     VTIRPRSCVFQFSQATVLAQFERCSTLAQAVREIGQAVAIEHFDGQSDALLRVSALLP
                     NFVKLDRSITQHLAAEPARQTQIRELVRKLKEGGAAVIAAYIEDAQSLGVLWQAQVEL
                     VQGNFVQQPDEVIQHQVTS"
     gene            complement(24476..25183)
                     /gene="bioD"
                     /locus_tag="PK144_01270"
     CDS             complement(24476..25183)
                     /gene="bioD"
                     /locus_tag="PK144_01270"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNF64381.1"
                     /translation="MNAPLSAGCFVTGTDTGIGKTRAAVALLQALAARGLRAVGMKPV
                     ASGCEATPAGLRNDDALALIAASAGAPDYALVNPYAFAPPVAPHLAARAAGCGIDFAH
                     IEDCARRLAAGADWLVVEGVGGWRVPLGAGGELATLAQRLGLPVVLVVGLRLGCLNHA
                     LLSADAILAAGLPLAGWIANRIDPDMALADDNLATLTALLPAPCLGTLAWCPLPASPA
                     GGLDLRPLLAAAAAQRS"
     gene            complement(25180..26082)
                     /gene="bioC"
                     /locus_tag="PK144_01275"
     CDS             complement(25180..26082)
                     /gene="bioC"
                     /locus_tag="PK144_01275"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNF64382.1"
                     /translation="MNDPLPPATRPDARLVRAAFDAAAARYDDCAVLQREVGTRLLER
                     LDLVKLVPARVLDLGCGTGLHTRALLDRYPQALVCGADLAPAMCARTRARSGWFRRLR
                     AVCADAARLPFADDSLDLVWSNFTLQWCGAPDAVFAEVRRVLRPGGLFLFTTLGPDTL
                     RELRAAWAAVDDRPHVNDFTDMHDVGDALVRARLADPVLDVERFTLTYPDVLALMREL
                     KAIGAHNVLDTRARGLTGRGRLAAMSAAYERERRDGVLPASYEVVYGHAWAPAQKLSV
                     DGPGGVATFPLSGLRRRPPAGPAA"
     gene            complement(26112..26870)
                     /gene="bioH"
                     /locus_tag="PK144_01280"
     CDS             complement(26112..26870)
                     /gene="bioH"
                     /locus_tag="PK144_01280"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HNF64383.1"
                     /translation="MLYCQSWGSGPDLVLLHGWGMHGGVWDDTVEAFIDDWRVTVIDL
                     PGHGRSGGRPGTLAGLAAAVAAVAPARAAWLGWSMGGLVAQRIALDAPARVDRLLLTG
                     SNACFVQRPDWPCAMTTAALAKFASGLADDPKATLERFIALETLNLPNARAHLRVLRE
                     IVFRHGLPATVALEDGLSILEAADLRAEWPRLGCPVRLLLGQRDALVPVAAGEALTAL
                     AADVRVQVFRHATHAPFLSHPDEFHAALATALHD"
     gene            complement(26890..28053)
                     /gene="bioF"
                     /locus_tag="PK144_01285"
     CDS             complement(26890..28053)
                     /gene="bioF"
                     /locus_tag="PK144_01285"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNF64384.1"
                     /translation="MRDLAAELAALRAAGLYRAPRTVDSAQGPVIAAGGREWLNFCSN
                     DYLGLAADARIAQALATAAARRGCGSGAAHLVCGHSAEHAALEEELAAFTGRPRALLF
                     STGYLANLGVLAALAGRGDSVLEDRLNHASLLDGGRLSGARLSRYAHADAEALEAKLA
                     VAGGERIVVTDGVFSMDGDVAPLPALADACRRHAAWLVVDDAHGFGALGPQGRGSLAL
                     HGLDAEAVPVVIGTLGKAFGTSGAFVAGSAELIEYLIQKARPYIYTTASSPALAAATR
                     ASLRIVQAEDWRRERLTALIARFRAGAAQLGFGARLLPSTTPIQPIVIGAAEDALAAA
                     QRLADRGIWITAIRPPTVPAGSARLRVTLSAAHTDAQLDRLLDALAAALPAPA"
     gene            complement(28070..>28963)
                     /gene="bioB"
                     /locus_tag="PK144_01290"
     CDS             complement(28070..>28963)
                     /gene="bioB"
                     /locus_tag="PK144_01290"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNF64385.1"
                     /translation="LSIKTGACPEDCAYCPQSARHEGEVEREKLLAVEEVLSAARTAK
                     ASGASRFCMGAAWRNPKDRDLDAVVAMVEGVKALGLETCVTLGMLRADQAQRLKDAGL
                     DYYNHNLDTSPEFYGSIITTRTYQDRLDTLGHVRSAGIKVCSGGILGMGESLRDRAGM
                     LVQLANLPEHPESVPINLLVQVEGTPLYGRDRLDGIDFVRTIAVARLLMPQSFVRLSA
                     GRTDMSDELQALCFSAGANSIFYGEKLLTTANPDTDRDRALFAKLGVRALEIQETLPE
                     AGDFVQEAPCGCGAAKLRAGA"
BASE COUNT         4499 a        10485 c        10061 g         3918 t
ORIGIN      
        1 atcagcgaga ggtcgtggcg cagcagcgtc gccaggcgcc ggcgcttctg gccggtgacg
       61 cgcgcatcct cgccgcgcca gaagtaacca tgatgcacga ggatcgcatc ggcaccgaga
      121 tcgagcgccg cttcgagcag cgcctgacag gcggtgacgc cgccgaccag cagccgcacg
      181 tcgggccggc cctcgacctg caggccgttc gggcagtagt cgctgaagct cgccgcggcg
      241 agcagcgtgt cgcagtagcc gaccagctcg cgcaggtgca tggcgtgccg cctcagccgt
      301 gcagcttcgc gagcgccgcg agcagcgcgg cgttctgcgc ggggctgccg accgtgatgc
      361 gcaggaactg ctcgatgcgc ggcagccgga agtggcggac gatgacgccg tgcgcgcgca
      421 ggccggcggc gaggccggcg gcatcgtgcg ccggatggcg tgcgaacacg aagttcgcag
      481 ccgagggcag cacctcgaag ccgtgatccg tcagcgcctg cgcgagctgt tcgcggctgt
      541 cgatgaccgc acgccggtgc acgtcgaagt attcgtcgtc ctcgagcgcg gccacggcgc
      601 cggcgagcgc cagccggtcg agcgggtagg agttgaagct gttcttgatg cgctccagcg
      661 cctcgatcag cgcgggatcg ccatacgccc agccgacgcg caggccagcg agcgcgcgcg
      721 acttcgacag cgtctgcacg accagcaggt tcgggtactg cgcgaccagc gcagcggcgc
      781 tgtcggcgcc gaaatcgacg taggcctcat cgaccagcac gaccgaatcg cggttgaagc
      841 ctagcaggcg ctcgatgtcg gcgagcgcca gcgcccggcc ggtcggcgcg ttcgggttcg
      901 cgaacacgat gccgccgttc ggccggcggt agtcctcgac gcgcagcgag aagtcgccgg
      961 cgagcgggat ggtctcgaag ccgatgtcgt acagcccgca ccagaccggg tagaagctgt
     1021 aggtcacgtc cgggaacagc agcggccgtt cgtgacgcag caggccgtgg aaggcatgcg
     1081 cgagcacctc gtcggaaccg ttgccgacga acacgtgcgc ggccgtgagt ccgtggcgtg
     1141 cggccagcgc ctgcttcagc cggcccgcgt tcgggtccgg atacaggcgc aggccctcgt
     1201 cgagggcggc gcggatcgct gccagcacgc gcggcgaggg tccgtccggg tgctcgttgg
     1261 tgttgagctt gaccaggttc ggtatctgcg gctgctcgcc gggcacgtac ggcgtcagcc
     1321 ggccgacgac ctcgctccag tagcggctca cgacgcggcc tccggcggca cgaagccggg
     1381 gatgcggtac tcggccgagc gcgcgtgcgc actcagcccc tcgccgcgcg ccagcaccga
     1441 ggccgtgcgt ccgagcgtgg cggccccgtc cggcgagcag aagatcaggc tcgaacgctt
     1501 ctggaagtca tagacaccga gcggcgacga gaagcgcgcg gtgcgcgagg tcggcagcac
     1561 gtggttcggc ccggcgcagt agtcgccgag cgcctcggcc gtgtgccggc cgaggaagat
     1621 cgcgccggcg tggcggatgc tcggcagcaa tgcctgcgga tcggcgaccg acagctccag
     1681 gtgctcgggc gcgatgtagt tggcgacctc ggccgcctcg gcgaggtcgg cgacccggat
     1741 cagcgccgcg cggctggcca gcgacacgcg cacgatgtcg gcgcgcgcca gcgtcggcag
     1801 caggcgctcg atggcggcgg cgacgcgatc gaggaaggcc gcatccggcg agatcaggat
     1861 cggctgcgcg tcctcgtcgt gctcggcctg cgagaacaga tccatcgcga tccagtcggc
     1921 atcggtgtcg ccgtcgcaga tcacgaggat ctcggacggt ccggcgatca tgtcgatgcc
     1981 gacctgcccg tacacggcgc gcttggcggt cgcgacccag atgttgccgg ggccgacgat
     2041 cttgtcgacg cgcggcacgc tcgccgtgcc gtaggcgagc gcggcgaccg cctgcgcacc
     2101 gccgatcgcg aacacgcgat cgacgccggc gaggcgcgcc gcggcgagca ccagatcgtt
     2161 ctgctcgccg tccggcgtcg gcacgaccat caccagctgc ctgacgccgg cgaccttggc
     2221 cgggatcgcg ttcatcagca ccgaggacgg ataggcggcc ttgccgcccg gcacgtacag
     2281 gccgaccgca tcgagcgccg tgacctgctg gccgagcagc gtgccgtcgg cctcggtgta
     2341 gctccaggaa tcgagcttct ggcgctcggc gtaggcacgc acgcgcgccg ccgcggtttc
     2401 gagcgccgtg cgctgcgccg gcggcagcgc gcagagtgcg gcatcgaggc gctcggccgg
     2461 gatttccagc tcggcggcag cggcgaaggc cgtgcggtcg tagcggttgg tcagttcgag
     2521 cagggccgcg tcgccgcgcg cacgcaggtc ggcgatgatc tgcgcgaccg tgccggcgac
     2581 gtgggcatcg gagacgccct cccaggccgt cagccggtcg agacgcggcc agaagtccgg
     2641 atcgctgtag ttcaggcggg cgatgtccac ggcggggggc tcctgcacgg caagcgcgtg
     2701 cgcaaggacg gaggggacgg atagggccgg cagcccggcc ggcctcaggc gacggcgcgg
     2761 ccggtcgcgg cggccatgtg ttcgaggatc gcgcgcacgc gcgcgtgctt catcttcatc
     2821 gccgcgcggt tgacgaccag ccgcgagctg atgtcggcga tcagctccag cggctccaga
     2881 ccgttggcgc gcagcgtctt gccggtatcg acgatgtcga cgatgcgatc ggcaaggccg
     2941 accagcggcg cgagttccat cgagccgtac agcttgatga tctcgacctg ccggccctgc
     3001 tgcgcataga agcggcgcgc gacgttggcg aacttggtcg cgacgcgcat gcgtccgcgc
     3061 ggctccggca cgcccggccg gccggcggtc atcagccggc agcgggcgat gccgagatcg
     3121 agcggctcgt agagcccctc gccgccgtgc tccatcagca cgtccttgcc ggcgacgccg
     3181 aggtcggccg caccgtactg cacgtaggtc ggcacgtcgg tggcgcggat gatgacgagc
     3241 ttcacgtccg ggcggtcggt gtcgaggatc agcttgcggc tggtctccgg gtcttcgagc
     3301 ggccggatgc cggccgcggc gagcagcggc agcgtctcct ggaagatgcg tcccttcgag
     3361 agcgcgatgg tcagcgtgtc ggacatggcg ggaggctctg actccggggg gctcagggca
     3421 cgcggcggat cagcgcgccg agactgccga gcttctcctc gatgcagtcg tagccgcgat
     3481 cgatgtgata gacgcgatcg acgacggtat cgcccttgcc gaccagtgct gccagcacca
     3541 ggctcgccga ggcgcgcagg tcggtggcca tgatcggcgc cgcggtcagc tgcgccacgc
     3601 cggtcgagat caccgtgttg ccttcgagct ggaactgcgc acccatgcgc gacagctcgt
     3661 tgatgtgcat gaagcggttc tcgaacaccg tctcggtgat cgtgccgacg ccttcggcga
     3721 ccgcgttcag cgcgcagaac tgcgcctgca tgtcggtcgg gaaggccgga tacggcgcgg
     3781 tgcggatgtg cacggccttc ggccgccggc cctgcatgtc gagctcgatc cagtccgggc
     3841 cggtcgtgat cacggcgccg gcttcctcga acttcacgag cacggcctcc agggtgtcgg
     3901 gccgcgtgtc cttgacgcgc acgcagccgc cggtgatcgc ggcggcggcg aggtaggtgc
     3961 cggtctcgat gcgatcgggc agcacgcggt agtgggctcc gtgcaggcgc tcgacgccgt
     4021 cgatgaccag cgtgtcggtg ccggcaccgt gcacctgcgc gcccatcgcg ttcaggcagt
     4081 tggcgaggtc gacgacttcg ggctcgcgcg cggcgttctc gaggatggtc gtgccctgcg
     4141 cgagcgcggc ggccatcatc aggttctcgg tgccggtgac ggtgacgagg tcgagcacga
     4201 tgcgcgcacc cttcaggcgc gggcagcggg cgcggatgta gccttcctcg acgcggatgt
     4261 cggcgcccat cgccgcgaga cccttgatgt gcaggttcac cggccgcgag ccgatcgcgc
     4321 agccgccggg cagcgacacc tcggcctcgc cgaagcgcgc gagcagcggt ccgagcacga
     4381 ggatcgacgc acgcatctgg cgcaccagct cgtagggcgc cgtgtggctg gtgatgcagc
     4441 gcggatcgac ctcgatgttg agcttctcgt cgacgaccaa ctgcgcgccg agccggccga
     4501 gcagttccat ggtcgtggtc acgtcctgca ggtgcgggac gttgccgatc gtgaccggac
     4561 cgtcggcgag cagcgtcgcg gccaggatcg gcagcacggc gttcttggcg ccacaggcgc
     4621 gcacttcacc gcgcagcgtc tcgccgccgg tgatgatcag cttgctcatg cgcgatccct
     4681 ggtcaggcct gcgcctgctg tgccgcccag gcctcgggcg tgaaggtgcg caggccgagc
     4741 gcgtggatct cctcgcgctg caggcgatcg ccgagcgcgg cgttgaccag ccggtgctgg
     4801 gcgatcagcg acttgccggc gaactcggcg ctgatgacga cggcctcgaa gtgcacgccg
     4861 tcatcgccgc gcacgagcac ctcggcggac ggcagggctt cctggatcag acgctggatg
     4921 tcggcggcct gcatgggcgg atacctgtgg aatcgaaagg gggaaaggcg gccagcgccc
     4981 cggaaggcgt accgcaagag gcgggatgat atcggaaaac cgccggactc aggcgtccgg
     5041 aaacagcgtg tcgaggtcgg agacggcgag gatcgcgcgc atctgggccg gcagtccgct
     5101 caggcgcagt tcgccgccgc cggcacgcgc ggcacgcagc cactcgacca gcagcgcgac
     5161 gccggcactg tcggcacggc gcacgccgtc gagactcagc gtcaccgtct cgccgccggc
     5221 gagttccccg gccgcggcgt cccacagcgc agcgaccgcg tcgatgacca gatcgccgcc
     5281 gagcgccagc cggccgggcc cgaggcgggt cagcgtcgcc ggctgcacgg ccgctgcgcc
     5341 agtgctcacg tcacctgccg ccgctggcgt tgagcttcgc gagcgaggcg atcagcccgt
     5401 ccatgccctg gtcgcggacc tggttggcga aggtcgtgcg gtagttggcg accagactga
     5461 ctccgtcgac ggtcacgtcg taaatcagcc actggccgtt cttctgatgg acgctgtagt
     5521 tgatcggtac gaccgggccg gcgcggcgca cgaactccga gcgcaccagc atgtcgccgt
     5581 cggcgttcgg cgcggcgccg ggcaggaagc gcacggtctc gtccgagtat tcgagcagcg
     5641 cattcgaata gatgcgcacc agcaggttct ggaactcggc cgtgaaacgc tggcgctgct
     5701 cgggactcgc gccacgccag ttgcggccga gcacccactg cgagatcgcc tcgaagtcga
     5761 agcgcggcac ggcgatgcgc tcgaccaggc cgtagatctt cgacggatcg gcgcgcagcg
     5821 cctcgcgctg ctgcttcagt gccaccagca tgcggctggc ggtgtcctcg accgcctgtg
     5881 ccgccggggt gtcggccgcc gtgcccggcc cggcggcggc gaagaggccg atgccgagca
     5941 gcagcgcgca cagcggtttc aggagtcggt gcaggatgga atgcatggcg gggggccttt
     6001 cgtcggattt cgggtgcagg gtcagacagc gaccggccgg tgcgttcagg gctgcccggc
     6061 cggccccggc gccggagccg gcgtctccgg acggccttcg gaagccttgc tgaacaggaa
     6121 ctggctgatc aggttttcga gcacgagggc cgactgcgtc agcttgatgg tcgaaccgtc
     6181 cttcagcacc gcgtcggcgc cgccggcctc gagtccgacg aactgctcgc cgagcaggcc
     6241 gctggtcagg atcttggcgc tggtatcggc cggcaggtta gcgtggcggg cggatatcga
     6301 cagttcgacg acggcctgga aagtctgcgg gtcgatgtcg atggccgtca cgcgcccgat
     6361 gcgcaccccg gccatcgaca ccggcgagcg caccttcagg ccgccgacgt tctcgaagcg
     6421 cgcgtggacg cgatacgcgg catccgcacc gagactggtc aggccactga cctgcatcgc
     6481 caggaacacc agggccgcca ggccgagcat gacgaaggcg ccgaccagca cctcgacggt
     6541 tcgcgacgga ttcatctagc gcactcctcc gaacagcagc gcggtcagca cgaaatcgag
     6601 cccgagcacg gccagcgagg tgttgacgac cgagcgcgtc gtcgcccggc cgatgccttc
     6661 cgaggtcggc tgggcgtcgt ggccctcgaa caccgcgatc cagttggcga ccacgccgaa
     6721 cacgaagctc ttgagcacca cgccgttgac cacgtcgcgc agcggctgga ccttggcctg
     6781 catctgcgcc caccaggcac cgtcatcaac accgagcagg ccgacgccga ccagatagcc
     6841 gccgaggatg ccgaccgccg tgaagatcgc cgccagcagc ggcacggcca ggatgccggc
     6901 gagaaagcgc ggcgccagca cgcggcgcac cggatcgacg gccatcatct ccatgcccga
     6961 gagctgctcg gtggccttca tcagcgcgat ctcggcggtc agcgccgaac cggcgcgccc
     7021 ggcgtagagc agcgcggcga ccaccggccc gagttcgcgc accagcgaca gcgcaaccac
     7081 cgtgccgagc gagccggccg cgccgaaatc cgccagcgtc gtatagccct gcagcgccag
     7141 caccatgccg acgaacaggc cggcgacgac gatgatcggc aggctcagca cgccgaccga
     7201 atacagctgg cgcaccagca gccgcggccg cggcagcacc gagggcaggc cggcgaggat
     7261 cgcgagcagc atcaggctgg cacgcccggt gcgcgcgaac accgtcagcg cccagcgccc
     7321 gagcgcggcg atcaagaccg tcctccgcca tcgagcagct cgctcgcgta gtcgcgcgcc
     7381 ggataatgaa acggcaccgg cccgtcgggc aggccgtgca tgaactgccc gacccagtcc
     7441 gaaccggaag cccgcagctc ggccggcgtg ccgcttgcga ccacacgccc gcccgagatc
     7501 aggtacacgt agtcggcgat cgagcagacc tcggccacat catgcgagac gacgacgctg
     7561 gtcatgccga gcgcgtcgtt gagcgtgcgg atcagctgca ccagcacgcc catcgagatc
     7621 gggtcctgac ccgtgaacgg ttcgtcgtag agcatcagca tcgggtcgag tgcgatggca
     7681 cgcgcgagcg ccacacgccg ggccatgccg ccggagagct ccgccggctt caggtcacgc
     7741 gcgccgcgca ggccgacggc ttcgagcttc atcagcacca gcgtgcgcag cagccgcgcc
     7801 ggcagccgcg gatagtgctc gcgcagcgga aacgcgacgt tctcgaacac cgacagatcg
     7861 gtgaacagcg ccccgctctg gaacagcatg ccgatgcgcc ggcgcagcgc gtacagctcg
     7921 cgccgcctaa gcgccggcac ctcgtgaccg tcgagcacga cgctgccggc tgccggccgc
     7981 agctgaccgc cgatcaggcg cagcagcgtg gtcttgccgg tgccgctcgg ccccatgacc
     8041 gccgtgaccc tgccgcgcgg gatgtcgaga tcgacgccat cgaagatcac ccgcgtaccg
     8101 cgcaggaagc gcaggccgcg gatgcgcacg agactgtcgg cagcagccgg ggagtcggga
     8161 acgtcgggca tcgggaaaac ggaagcggga ctcggtcaaa gcgggtgaaa ccggcgagcg
     8221 ggacgatgcg ggcgcggcag acgggcatgc tgcaatacgg tagtgtcacc cgtgccgccg
     8281 agcgccgtca aaccgctgag ccccggcagc ccgtgtatga tgccccgccc gcatgccccc
     8341 ggcctgctcc tgcttcccgc ccctttccgt caccgtcccc atgaatcccg accggcttcg
     8401 cgacctcgga cgcaccgcgc tgcgcatcga agcgcaggcg atcgagcacc tgatcgagcg
     8461 cgtcgatgcg gccttcgtcg ccgcctgcga gtgcctgctc gcctgccgcg gccgcatcgt
     8521 cgtgctcggc atcggcaagt ccggccacat cggcggcaag ctggcagcga ccttcgcaag
     8581 caccggcagc ccggcgttct tcgtgcatcc ggccgaagcg agccacggtg atctcggcat
     8641 gatcaccgca caggacgtgg tgctggcgct gtcgaactcc ggcaccaccg acgagctcct
     8701 gatgctgctg ccgctgatca agcgcctcgg cgtgccgctg gtggcactga ccggcaaccc
     8761 ggagtcgccg ctggcccgcg ccgccgacgt gcacctcgac gtgagcgtcg cgcaggaggc
     8821 ctgtccgctg aacctggcac cgaccgcgag cacgacggca gcgctggcca tgggcgatgc
     8881 actggcggtg tcgctgctcg aagcccgtgg cttcacggcc gatgacttcg cgcgctcgca
     8941 tccgggcggg cggctcggcc ggcgcctgct gctgctgatc gacgaactca tgcacaccgg
     9001 cgagcgcctg ccgcgctcgc ggccggacga tccgctgccg gtggcgctgc tggagatgac
     9061 gcgcaagggc ctcggcatga ccatcgccac cgacgacgac gaccgcatgc tcggtgtgtt
     9121 caccgacggc gacctgcgcc gcgcgctcga ccggcgcatc gacctgcacg ccgccacgct
     9181 cgccgaggtc atgacccggc cgggcttcag cgtgcgcacc ggcctgctcg ccgccgaagc
     9241 cctgaacctg atggagcgtc accgcatcaa cgcgctgccg gtgctcgatc gcgacgaccg
     9301 gctggccggc gccctgaaca tgcacgacct gctgcgcgca ggcgtgctct gagggagcca
     9361 tccggcgcgt gcgcacgctg ctcgtcctcg tcgcgctggc cctgctcgcc gggctgagcg
     9421 gctggttcct gaacgatctc gaacgcgacc tgcgcgaggt gcgcgcacgc gacgaccgcc
     9481 tgccgctcac catcgccgaa aacttcgtgg ccacggtcat gaacgcccgc ggcgaacggc
     9541 tgtacgtgct caccggtccg cacctcgaac agtttccggc cccgcgcggc accttcgtga
     9601 cccgcccgct cgcgcacacc tacgagaatc tggcgccggt ctgggaaatc cgcgccgaac
     9661 agggctggat cgctcccggc accgaaacca tcgaactgcg cggcgacgtg catgccgagc
     9721 gtccggccag caccggttac gtgccggtga tcatgaccgg cagcaacctg cggctgatcc
     9781 ccgacaagaa ctacgccgaa tccgccgagc ccgtgcgtgt gcagaccccg gatggcgtca
     9841 tcgacgccgt cggcttccac gcctggctcg acgaggatcg catcgaactg cttgccgacg
     9901 tccgaggaca ctatgcgccg cctgcccaga aacctgatcg cccctagccg gatcacccgt
     9961 ggccggatca cgtccggcct gatcgccccc ggcctgctcg cctgcgccct gagcgcccag
    10021 gccctgcccg aagaccgcca gcaaccggtc aacctggtcg ccgaccgcgc cgagatgaac
    10081 cagcagaccg gcgtcgccac ctacgagggc aacgtcatcg tcacgcaggg cacgatgcgc
    10141 ctgaccggtg acaaggtcgt ggtctacacg caggagggcg aactgcagcg catggagagc
    10201 ttcggcaagc cgacgacgtt caagcagaag ccggccgccg acaaggagga aatcctcggc
    10261 gagtcgctga agctcgaata cgacgccagg accggcatcg tcatcgccac cggcaaggcg
    10321 aagatcacgc aggtcaagga caccttcacc ggcgaccgca tcgagtacga cgtcaacaag
    10381 gacctcgtga aggcccgcgg cggcgagagc gccggccgcg tgcagatcat cctgcagccc
    10441 aagcccgaag gcggcacgcc ggtcccgggc gccagacccg cgcagccgaa gaagccctga
    10501 gccatggccc gcctcgaagc gcgcgaaatc gccaagcgct acaagggccg gcgcgtcgtc
    10561 gatgccgcca gcgtgcacgt cgacagcggc gaggtcgtcg gcctgctcgg gccgaacggc
    10621 gccggcaaga ccacgagctt ctacatgatc gtcggcctga tcggctgcga cgaaggtcat
    10681 gtgctgctcg acagccgcga gatcacgcag ctgccgatgc atgcgcgcgc gcgcctcggc
    10741 gtcggctacc tgccgcagga agcctcggtg ttccgcaaga tgaccgtgct cgacaacgtg
    10801 ctcgccatcc tcgaaacccg cggcgacctc gaccgcagcg cgcgcaccga acgcgccgag
    10861 accctgctgc acgaactgaa cgtcggtcac ctgcgcgaca acctcggcat ctcgctgtcg
    10921 ggcggcgagc gccggcgcgt cgagatcgcc cgcgccctcg ccgccgagcc gcgcttcatg
    10981 ctgctcgacg agcccttcgc cggcgtcgat ccgctgtcgg tgcacgacat ccagcgcatc
    11041 gtgcgccacc tctgcgaccg cgacatcggc gtgctgatca ccgatcacaa cgtccgcgag
    11101 accctgggca tctgcgatcg cgcctacatc ctcgccgaag gccgggtgat cgccaccggc
    11161 acgccggcgg aaatcctcgc cgaccggcgc gtgcgcgagg tctacctcgg cgagcacttc
    11221 cggctctgaa gcggcctgcg tcacttgcac gccgctggca tatggcgtgc tataacccga
    11281 ccatgatcct cgtgcgccgt cagcccccgc cgtagtcccg atgagacagt cgctgcagct
    11341 ccgcctcggt cagcacctga ccatgacccc gcagctgcag caggccatcc gcctgctgca
    11401 gctgtcgagc cttgacctgc aggccgaaat ccagaccgtg ctcgactcca acctgatgct
    11461 ggagcgcagc gacgacaccc cggacccgga agtgccgacc gccgaagcgg cgctgagcga
    11521 aaccgtgccg ccggcccagg accgcaccga gaccccgacc gacacgatcc cgaacgaact
    11581 gccggtcgat accgaatggg gcgatgtcta cgacagctac gacggctcga ccagctattc
    11641 gcgcggcgat gacgacgaca accgcgacct gttcgagaac cacggcaacg accagccctc
    11701 gctgcaggaa cacctgctct ggcagatgcg cctgacgccg ttctcggcgc tcgacgccga
    11761 gattgccgaa gccctgatcg acgccaccga cgcctccggc tacctgacgc tgacgctcga
    11821 cgacatcctc gaaggcctgc gccgcacgca cccggacctg gagcgcgacg aggtcgaggc
    11881 cgtgctgcac cgcgtgcagc acttcgaccc gcccggcatc tgcgcgcgca atccgtccga
    11941 atgcctgatg ctgcagctcg acacgctgcc gggcgacacg ccgtggcggc gcgaggcgat
    12001 cgagctcgtg cgccagtacc tcgaactgct ggccggacgc gacttcaacc tgctgatgcg
    12061 gcgcatgaag ctcacgcgcg agcagctgca ggaagtcatc gcgctgatcc agaccctgaa
    12121 cccgcacccg ggcacggcgc tgtcggccga gacggccgcc tacgtcgtcc cggacgtgtt
    12181 cgtgttccgc ggccgggacg gctggcgcgt cgatctcaac cccgacagcg cgccgaagat
    12241 ccgcatcaac gccgaatacg cgcgcctcgt gcgccgggcc gactccagcg ccgacaacca
    12301 gtacctgaag aaccacctgc aggaggcgcg ctggttcctc aagagcctgc gcaaccgcca
    12361 cgacacgctg ctgaaggtcg cgcgctgcat cgtcgaccgc cagatctcgt tcttcgagaa
    12421 gggccccgag cacatgcgcc cgctggtgct gcgcgacatc gccgaggccg tcgagatgca
    12481 tgaatcgacg atctcgcgcg tgaccacgca gaaatacatg cacacgccgc gcggcatctt
    12541 cgagttcaag tacttcttct ccagccacgt cgccacgtcc gacggcggcg aggcctcctc
    12601 gatcgcgatc caggccatga tccgcaagct gatcgccgcc gagaacaccc agaaaccgca
    12661 cagcgacagc cggctcgccg aactgctggc ggccgaaggt attcaggtgg cccggcggac
    12721 ggtggcaaaa taccgggaag ccatgtcgat cccctcctcc acagaccgca agcgcatcgg
    12781 ctgacgcagt gccggcgcga cccacgcggc aaccggagcg gctgcacgga cgcctccacg
    12841 aaccacggac cggcccgctc ctccacccaa gccaggagta caccatgcaa atcaacctga
    12901 ccgggcatca cgtcgagatc tcccagccgc tgcgcgacta cgtgcaggac aagatcggca
    12961 agatcgagcg ccactgcgac agcatcacca gcgcctacgt gattctcagt gtcgaaaagc
    13021 tgcgccagaa ggccgaggcg accctgcacg tcgccggcaa cgacatccac gccgatgcca
    13081 tcgaggagga catgtacgcg gcgatcgacg tgctggccga caagatcgac cgccagatca
    13141 agaagcacaa ggaaaagctg accgaccacc accgcagcga caaggccacc ggtaccccga
    13201 gctgatccgc ctttcccgtc cctcgcgcgt cccgggctcc tgcatgaaca tctccgatct
    13261 cctgactgcc gggcgcgtcg cctgccgctc ggacgtggtc agcaagaagc gcgtgctcga
    13321 gctggtcagc gagctgatcg cggccggagc gagcggtgtg gatgcccggg cgatcttcga
    13381 cagcctgatc gggcgtgagc gcctcggcag caccggcctc ggcaacgggg tggcgctgcc
    13441 gcacgggcgc ctcgccggca ccgaacaggc cctcggagcc ttcgtccggc tcggcaaggg
    13501 cgtcgagttc gacgcgatcg acggccagcc cgtcgatctc gtgttcgcgc tgctcgtgcc
    13561 ggaacacttc accgacgaac acctgaagat cctcgcgcat ctggcggaga tgttcagtga
    13621 ccgggcgttc tgcgagcgcc tgcgcacgac ctcggccgac gccgacctgc atgcgctgct
    13681 gaccggctgg tcgccgtccg cagacgcggc gctctgagca tgatcgcagc gctgccggcg
    13741 gatcaggcgt tcgcggcgct gcgtgccgcg ctggccctgc agtggatcgg cggacgcagc
    13801 gccggcgcgc gcctgatctg ccgcgaaggc gaaagcctgc cgccgcgttc gctcggcggg
    13861 ctgctgaacc tcgtcaccgc gccgcgcgtg caggtgctcg ggcgcgagga actggcgttc
    13921 ttcgcgagcc tcgatgccgg cgcgcgtgcc tcgctgctcg accggctgct ggcgacgccg
    13981 gtcggcgcca ccgtcctctg cggcgactcg ccgctgatcg aggagttctc cagctacgcc
    14041 gaccggcacg aatccccgct ctggcagacc tcgtcgacac ccggcgaggt gctcgatcgc
    14101 ctgtccgaat accagcacga gctcgacgcc agctcgaccg tgctgcacgg cgtgttcctg
    14161 gaggtctacg gcctcggcgt gatgctgtcc ggcgaatcga gcaccggcaa gagcgaactg
    14221 gcgctcgagc tgctgtcgcg cggccaccgg ctgatcgccg acgacgcgcc gaagctcacg
    14281 cgcgtcgcgc cgcaggtcat cgagggcacc tgtccggatg cgctgcgcaa cttcctcgaa
    14341 gtgcgcggcc tcggcatcct gaacgtgcgc cggatgttcg gcgacagcgc gatcaagcgg
    14401 aacaagcgcc tgcgcctgat cgtgcatctg gtgcgcctcg aagaaacctc gctgccggcc
    14461 gaggtgcgcc tgagcggcgc gcgcgcgacg cgctacatcc tcgatgtggc gatccccgag
    14521 atcacattgc cgatcgcacc ggggcgcaac ctcgcggtgc tggtcgagtg cgcggtgcgc
    14581 gatcacatcc tgcgcctcgg cggctacaac gccgagcgcg acctcatgga gcgcctgcag
    14641 gacgagatga ccggaggcgc ggcgtgaggc tgatcgtggt cagcggcctg tccggctcgg
    14701 gcaagagcgt tgcgctcgca acgcttgagg actgcaacga ctactgcgtc gacaacctgc
    14761 cgctcggcct gctgccggcc ttcgccgagg aactgctgac gcaccagcgg ccgagcctcg
    14821 aaaccgccgc cgtcggcatc gatgcccgca acttccgcga ccagctcgaa cgcttcccgg
    14881 acatcgtcgc gcagctgcgc gccgcgcgct tcgaagtcga catcgtcttc ctgcaggccg
    14941 atgccgaaac cctgctcaag cgcttcagcg acacgcgccg cccgcacccg ctcagcgtcg
    15001 agcacggcac gccgctgctg gaggcgatcg aggccgaacg ccggctgctc gcgccgatcg
    15061 ccgcgcacgc gacgctggtc atcgacacca gccacaccaa cctgcacgag ctgcgcgagc
    15121 tgctgcgcac gcgcctgcac ggcggcgaga tcggccagct gtcgctggtg ttcgagtcct
    15181 tcggcttcaa gaacggcctg cccgacgatg ccgactacgt cttcgacgtg cgcttcctgc
    15241 cgaatccgca ctggcagagc gcgctgcggc cgctgaccgg ccgcgacgag gccgtcgccc
    15301 gcttcctcga agcccacgag gtcgtcgagt cagccgtcgc cgacctgatc cgcttcctcg
    15361 aaacctggct gccggccttc gagaccggca gccgcagcta tctcaccgtc gccgtcggct
    15421 gcaccggtgg ccagcaccgc tcggtctacg tcaccgaacg gctggcccgc catttcggcg
    15481 ccacccgccg cgccgtctcg gtgcgccaca gggaactgtc atgagcgtcg gactcctgct
    15541 gatcacgcac aaccgcctcg gcgatgaact gctcgccacc gccaccacca ccttcgcaca
    15601 ctgcccgatg cgcgccgcca ccgtgcgcgt cggcgagcgc tgcgacccgg aggcgctggt
    15661 cgccgaggcg cgcggcaagc gtgccgaggt cgacgacggc agcggcgtgc tggtgctgac
    15721 cgacatctat ggctcgaccc cgtcgaacgt cgcacaccgc ctggtcgacg acggcggcgt
    15781 gcgcgtgatc gccggcgtca gcctgccgat ggtgatccgc gtcatgaact actgccggct
    15841 cgacctgctc gccctcgcgc acaaagccgt cagtgccggt cgcgacggca tcgtgctgac
    15901 ccccgaagac gacgccgccg tctgaccctc gcccgccccg acccgtccgg aatccgaccg
    15961 ccgtgatcca gcgcgatctc accatcgtca acaaactcgg cctgcatgca cgggccgccg
    16021 ccaagttcgt gaccaccgca tcgtcgtatc aggcggcgat ccaggtcacc aagggcggac
    16081 gcatcgtcaa tggcaagagc atcatgggcg tcatgatgct cgccgccggc aagggctcgg
    16141 tgatcacgct gagcgccgac ggtcccgacg ccgaagccgc gctcgacggc ctcgcgacct
    16201 tggtcgccga ccgcttcggc gagggcgagt gagcgtacgg ccatgccgct cgccctgcac
    16261 gggatcggcg tatcgcgcgg ctgcgcgatc ggggagacct atcacctgct gcgtgaccag
    16321 ccggaagtgc tggagtacgc catcccgcag gacatggtcg atgacgaggt gcgccgcttc
    16381 gaggaggcca tcaccgaagc ccggcgcgag ctggaggaca tccgctcgcg cgtcgcgccg
    16441 aacgtgccgg ccgaggtcac gtcgttcatc gacgcccacc tgctgatgct tgaggacccg
    16501 gcgctcgccg atgcgccggt gcgcctgatc cgccagcaca gctgcaacgc cgaatgggcg
    16561 ctgaagatgc agcgcgacct gctcgtgcag gtgttcgacg acatggacga cgcctacctg
    16621 cgcgcccggc gcgacgatgt cgatcacgtc gtgcgccgcg tgcagggcat cctgctcgcc
    16681 gaggaaccgg caccggacct gctcgcgagc cgccttgaag gccgcatcat cgtcgccgat
    16741 gacctgacgc cggccgacac catcctgatg cagcaccagg gcgtggccgc cttcgtcacc
    16801 gaatacggcg gcccgctgtc gcacacggcg atcctcgcgc gcaacctcgg catcccggcc
    16861 gtggtcggtg cgcgccatgc gcggcgcctg ctcggcgacg aggaaaccat catcgtcgac
    16921 ggccagaacg gcgtcgtgct cgccgaactc gacgagccgg tactcgaaca ctatcgcgag
    16981 cgccagcgcc gcgagcagcg cggccgccgc gaactcgtca agctgcgccg gctgccggcg
    17041 atcagcaggg atcaggtcac gatcggcctg caggccaaca tcgagctgcc cgaggacgcc
    17101 ctggccgtgc gcgagatcgg cgccgacggc atcggcctgt accgcaccga attcctgtac
    17161 atgaaccggc cggacacgcc ggacgaggag gaacacctcg cctcctacct gcacgtgatc
    17221 cgcacgctcg gccccgagca gccgatcacg atccgcaccg ccgacctcgg cgccgacaag
    17281 caggtcgacg gcggccggcg cgggccggtc gccaccaacc cggcgctcgg cctgcgcggc
    17341 atccgcctgt gcctgaagga tcacgagatg ttccgcgcgc agctgcgcgc catcctgcgt
    17401 gcctcggccc acggcaaggt gcggatgatg atcccgatgc tgtcgaacgc ctacgagatc
    17461 acgcaggtgc tgcacctgct cgacgactgc cgccaggagc tgcgcgcccg cggccaggcc
    17521 ttcgacgagc agctgccggt cggcggcatg gtcgaggtgc cggccgccgc ggtctgcgca
    17581 cggctgtacg cgcaccggct cgacttcctg tcgatcggca ccaacgacct gatccagtac
    17641 acgctggcga tcgaccgcat cgacgacgag gtcaactacc tctacgaccc gctgcatccg
    17701 gcggtgctgg cgctgatcgc cagcaccatc accgccggcc aggccgccgg catcccggtg
    17761 tcgatgtgcg gcgagatggc cggcgacgcc cgctacacgc ggctgctgct cggactcggc
    17821 ctcacacagt tctcgatgca cccgaacaac ctgctggagg tcaagcgcgt gatccgcgag
    17881 gccgaagtcg gggcactggc cagggaaacc gccgcgatcc ttgccgaaac ctcgcccgag
    17941 gtgctgagcg cccggctggc ggccttcgtc gcgtgaacgc cgtccctgaa agcggccggc
    18001 tcgatcgtgg cgacggccac cagctcgcct gggtgcgcac gcccggcacg acgccgggcg
    18061 tgatgttcct cggcggcttc cagtccgaca tgaccggcac caaggcgctg gcgctcgaag
    18121 cgcactgccg tgccgccggc cgggccttcc tgcgcttcga ctattacggc cacggcgcct
    18181 ccagcggccg cttcgaggac ggcacgatcg gccgctggcg cgccgacgcg ctcgcggcct
    18241 tcgacgcgct gaccgcaggc ccgcagctgc tggtcggctc gtcgatgggc ggctggctgg
    18301 cgacgctgct ggcgctcgat cgtgcgcaac gcgtcgccgg cctgctgacc atcgccgccg
    18361 ccgcggattt cgtcatcgac ctgctgccgg cgcgcctcag tcccgatcag cagcagcaat
    18421 tggcccgtga cggccgtatc gaactgccgt cgatgtactc cgacacgccg tatccgatct
    18481 gcgcccggct gatcgaggaa ggccgcgacc accggctgct gcacgccccc gtgccgtatc
    18541 acggcccggt gcgcctgctg cacggcagcg ccgatgccga cgtgccgtgg cagaccagcc
    18601 tgcgcctgct ggagcggctg gaaagccacg atgcgcggct ggtgctggtc aggggtgccg
    18661 atcaccgctt cagcgacgcg gccggcatcg cgctgatgct ggcgacgctc gaagaactga
    18721 tcggggcggc ggcggccggg ccgggctgag gcaccggact actgccgccg gcatccggaa
    18781 ggcccggccg gcgcggcggg gtcagatcat cgagccgtgg tgcagccatt cgtccggctg
    18841 ctgcacgaag ttgccctgca cgagttcgat ttccgcctgc cacagcaggc cgagcgactg
    18901 cgcatcctcg acgtagccgg cgatgatgcg cgggccggtg ctgcggatgc ggtgcacgag
    18961 ttcgcgcatc cagttctggc ggccggggtt ggtcgcgagg ttggccgtca ggctgcggtc
    19021 gagcttgacg tagtcgagcg gcaggttcgg cagcagcgcc agcgattcct cggcggcggc
    19081 gaagtgttcg agcgccgtgc cgtagccggc ggcgcgcacg cggcgcagca gttgttcggt
    19141 gcgccgcggc acgaccagca gcgtcgcctc ggagatctcg aacacgcagc tgcgcggctg
    19201 caccgagacc ttggccagca tcgcctccag ccactcggcg aagcccggat cggcgaccga
    19261 ggcgcccgag agcttgatga agaagcaggt gtcgccctgc tccttcagcc ggccttcgag
    19321 cccgcgcagc gcgcggctga ccacccagcg atcgagcagg cgatcgaggc cgaggcgggt
    19381 cgcggcgccg aggaactgct cgggcagcag cgtgcggccc tcggtgtcac gcatgcgcag
    19441 caggacttcg tagcgctcgc cgtggtcacc ggcgaggctg gtgatcggct ggtacatcag
    19501 gaacagccgg ccttccttga tcgcgccgct gatgcgcccc atccagtcgc gctgctcctc
    19561 gccgccgggg ccgccggcca gcaccggatg caggcgcacg cgatcgccgc cctcgcggcg
    19621 cagcgccaca cagacggcct tgccggcctc gaccgactgt tcgagcatgc cggattcggc
    19681 gcgcgcgacg ccgacataga cgctcgggcg gttgccgtgg ccgagctcat cgagcgtcac
    19741 gcagccgagc gcataggcca cgtcgcgcgc gaagctttcg agcttcggca gatcgacccg
    19801 gcgcgcgaac accagaaagg caccatcggc gttcatgccg gcgatgtcgc tgccgagcag
    19861 ctggtcatcg agcgcatcgg cgaagcgcgc acgccagcgc gcgcggcgca gttcgctcat
    19921 gtcgccctgc agcaggttgt cgacggccac gtacagcagc gcgccgatgc cggcgtcgtt
    19981 gcgcgcgagc gccgtctcga ccagcttgcg gaaggcctcg accgtcgcca gcccggagct
    20041 ggcgtcgcgg tgcgtgaagt cgcggatcgc cgaaccgtgg cggcggcgct ggccggcggc
    20101 atgggcgcgc actgccccga ccagcaggcc cggtgcggtc ggcttcggca gcacctcgac
    20161 gccgccgccg gtcagcaccg gcaggcggtc ctcctcggcg gcgagcagca gcaccggcag
    20221 ctgcgcgagc gcgggctcgt cgcggatcag gcgcatcagc gtcggtgcgt cggcctcggg
    20281 gatgccggca tcgaacacca gcacgtcagg ctgttcctcg gcggcggttt ccagcagcag
    20341 gcccggatcg tcgagcgtgc gcacgtgaca gccgctgccg cgcagcatgc cggcgagccg
    20401 ctccaggcgc accggatcgg catcgagcag cagcacgcgg tgcggctgcg gctggcggtt
    20461 gccgatcagc gcgtgcagcc ggcgcgcgag ccgggcgcca ccgagcggtt cgaccagaca
    20521 ggcatcggcc ccggcggcga gcgcctgcag ccggcagcgg ctgtcggtct gcggcgccac
    20581 cagcaccacc ggcaggtcct cgtgcaggcc ggcgaggcgc agttcggaga ccagccgggc
    20641 cccggcgcgg ggatcatggg cgtcgccgag cagcagcacc gcctgcggca gctgcagcga
    20701 cagcgccaga tgcagggcct gcaggtcatc gaaggtttcg gccttgtagc cgtgttcgcc
    20761 gagccgcgtc gtgtagccgg ccatgcgttc gggcgcgtcg ccgaagacgt agacgcgcgg
    20821 gcggtggtca ccggtctcgt cgcgggccgg gccggtggca ccggccttgc gtgcgaccgg
    20881 caccggctcg gcttcggggc cgacgatcgc gtcgtgcagg cggcggattg catcggcgag
    20941 ccgcacctgc tcgatcgaat ccggacgttc gccgctgcgc acgagttcac gcagcagcgt
    21001 gtcgacggtg cgcacgcgca cggccaggcc gcgttcatcg agcgagaggc attcctcgac
    21061 caggcgtccg gtgatctcgc tgacggcgtt cagggcctcg gccgtacagg cctgggccgc
    21121 cgcctgcccc cagcgcagca gcagttcgcg ggccagcgcg acgagctgga tgcgttcgcg
    21181 ctcgacgccg gcgctgacgg ccgtgcgatc gcgtgcagca cgctcgactc ccatgtccca
    21241 cctcctgaat agacgtttgc agcggctgca acggttgccg gcagccgtcc ctggacgttc
    21301 cggccagttt gctacgatcc ctgcacccca caccagaccg gagttccgcc gatgcgtgtc
    21361 ctgacccgcc tgtcgctgct ctgcctcgcc gccgtgcttg cccggggggc gcacgccgag
    21421 gagatcggca gcgtcgatac ggccttcaag ctgctcggtg ccaaccacaa gatcgtcgtg
    21481 gaagccttcg acgatccgaa gatcgacggc atcgcctgcc acgtcgccaa ggcccagacc
    21541 ggcggcatca agggcagcct cggtctggcc gaggacagct ccgatgccag catcgcctgt
    21601 cgccaggtcg gcccggtggt gttcagggaa agcttccgcg acggcgagga ggtcttcacc
    21661 gagcgccgtt cgatcctgtt caagaagctg cgcgtcgtgc gctttctcga ccgcaagcgc
    21721 agcacgctgg tgtatctggt ctacagcgac aagctgatcg acggctcgcc gaagaattcc
    21781 ctgagcaccg tgccggtgat gccgtggccg accggcagcg tcgccctgcc gccgccggcg
    21841 cgctgaaccg cgacggcctc agctcgtgac ctggtgctgg atcacctcgt cgggctgctg
    21901 cacgaagttg ccctgcacca gttcgacctg cgcctgccac agcacgccga gcgactgcgc
    21961 atcctcgatg taggcggcga tcaccgccgc gccaccttcc ttgagcttgc gcaccagttc
    22021 gcggatctgc gtctggcgcg ccggctcggc ggcgagatgc tgcgtgatcg aacgatcgag
    22081 cttcacgaag ttcggcagca gcgccgagac gcgcagcagc gcgtcggact ggccgtcgaa
    22141 atgctcgatc gccaccgcct gcccgatctc gcgcaccgcc tgcgccagcg tcgagcagcg
    22201 ctcgaactgc gcgagcaccg tcgcctgcga gaactggaac acgcagctgc gcggccggat
    22261 cgtcaccttg ccgagcatct ggcgcagcca ctccgggaaa ctcggatcgg cgaccgaggc
    22321 cgtgccgagc ttgacgaaga acaccgtgcc ggggtgatcc tgctggcgcg catcgagtcc
    22381 gcgcagcgcc cggctgacca cccagcgatc gagcaggcgg tcgagcccga acttggcagc
    22441 aaccggcagg aactgccccg gcagcagggt ctggccggaa gcatcgcgca tgcgcagcag
    22501 gacttcgttc cactcgctgc gatcggcgac cagcaggttg gtgatcggct ggtagaccag
    22561 gaacagccgg tgctcgcgga tcgcgtcgtt gatgatcgag tgccagcgcc gctgctcctc
    22621 gagatccggc gcgctgctcg acaccagcag cggctgcagg cgcacgccgg caccggcctc
    22681 gcgcgcctgc tgcgcggcaa gctgcgcggc atcgccgatc aggccatgca ccggcccggc
    22741 gtcggcgcgg cagacgccga tgctcggcac cagccgcggc cccggaccga gatcggacag
    22801 ctcgacgccg ccgatcgcat cgatcagcgc ccgcgcgtag ctctgcaggt tcggcagatc
    22861 gggctggcgc gtgctgatga tcacgcgccc ggcgttgtcc cagccgacga tgtcgttgcc
    22921 ctgctggcgc tcgctgatca cgcgcgcgac gcgcgtgcgc acctgttcga ggcgcgccgc
    22981 gtcgaggcgt ttcgaccagt cgagatagcc gccgaggctc acgtacagca gcgccgacag
    23041 cgcgccctcg gcctcgcgcg gatgcgcgag ccgcgcttcg agcgcttcgg tgaactgcgc
    23101 catcgtcagc aggccggtga cggtgtcggc gcgatcgggc cggcgcgcgc gcgagaagtt
    23161 ctgccggtac tgcatcgccc gtgcacgcac gcgctcgatg aagcgcaccg ggtcggcggg
    23221 cttgacgatg acatcgacgg caccggcgac gatcacgcgc gcatgcgcgg tcggatcgct
    23281 ctcgccggag agcagcatca ccggcgtgtt cgcgaactgc ggttcctcgt ggatcagccg
    23341 cgtcagctcg cagccgtcgg cctcgggcat gtagagatcg agcacgaaca catcgggctc
    23401 gaagccctgt gcggcttcga gcacgccgag cggattcgac agcgccttga cctcgaagcc
    23461 ggccgcctgc aggcggctcg actgcagctc caggaaggcg cggtcgtcat cgaccatgac
    23521 gacgcgatac ggcgccggct cctggtggct gatcagctcg ccgagccggg tggccagttc
    23581 cggcgcggcg accggcttga cgaagtagcc gacgccgccg gcgcgcagcg cgatcagctt
    23641 ggtgcggctg tcggcgcgcg ccgacaggaa cagcaccggg cagagcagct tgccggcgcg
    23701 gcgcagctcg ctgacctgat cggcaccgcc gaacacgccg tgcggcgcgt cgatgtcggc
    23761 gatcagggcc gccggctggc ggctggccat cgcatgcacc agcgcatccc agtcgacgaa
    23821 ggattcggcc gcatagccct gggcggcgag ctgcagcgac aggaacttcg cctgcgccgg
    23881 ctcggaatcg accaggaaca cgcgcggcgc gctggccggt gccgaggcgg ccaccgtcat
    23941 cgacgcgctc tcgggcgtgc ggccggtgct ggccgactgc gacagcgccg ccgccagtgc
    24001 ctcgacgcgc tcgccgagcg cctgcgcgcg tgccgggtcg ggacgcttgc cgctccagcc
    24061 ggcctcgcgc agcgcggtgt cgagcgcctg cgcctgtccg gccagcgcga agccggcggc
    24121 ctgcgcgcag gtgtcggcca gcccgcgcac gaacatgcgc aggtcgacca tctcggcggc
    24181 ttcgaagtgg ctgctgcggg cgcgctgcca gtggcgacgc agatcgctgg ccagcgtcga
    24241 cagcagcgtc cggtcctcgt cgcgcaggac gagcccgggt tcacggctgg ctggcaacgg
    24301 ttcgagcggc atcactccct cctgactgga caggccggcc atcttaccga tggcggccgg
    24361 cgcccgggcg ccggcgtcac cggcctccgc tgcgggcgct gtaaacaaaa tacacggcag
    24421 ccggcagccg gctggatgat ctggcaagcc agactagcac cggcaccggc cgcaatcatg
    24481 aacgctgcgc cgcagcagcg gcgagcagcg ggcgcaggtc gaggcccccg gcggggcttg
    24541 ccggcagcgg acaccatgcc agcgtgccga gacagggcgc cggcagcagg gcggtgagcg
    24601 tggcgaggtt gtcatcggcc agcgccatgt ccggatcgat gcggttggcg atccagccgg
    24661 cgagcggcag gccggcggcg aggatggcat cggcactgag cagcgcgtgg ttcaggcagc
    24721 cgagccgcag accgacgacc agcaccaccg gcaagcccag acgctgcgcg agcgtggcga
    24781 gttcgccccc ggcaccgagc ggcacgcgcc agccgccgac gccctcgacc accagccagt
    24841 cggcaccggc cgcaagacgg cgggcgcagt cttcgatgtg cgcgaagtcg atgccgcagc
    24901 cggccgcgcg cgccgccaga tgcggcgcga ccggcggcgc gaaggcgtag ggattgacca
    24961 gcgcgtagtc cggcgccccg gccgaggccg cgatcagcgc cagcgcgtcg tcgttgcgca
    25021 ggcccgcggg cgtggcctcg cagccgcttg cgaccggctt catgccgact gcgcgcaggc
    25081 cgcgcgccgc cagtgcctgc agcagcgcca ccgccgcgcg cgtcttgccg atgccggtgt
    25141 cggtaccggt gacgaagcag ccggcggaca gcggcgcgtt cacgccgccg gcccggccgg
    25201 cggccggcgg cgcagcccgg acagcgggaa ggtcgcgacc ccgccggggc catcgaccga
    25261 cagcttctgc gccggcgccc aggcgtgacc gtagacgacc tcgtaactcg ccggcagcac
    25321 gccgtcgcgg cgctcgcgct cgtaggcggc gctcatcgcc gcaagccggc cgcgaccggt
    25381 caggccgcgc gcgcgcgtat cgagcacatt gtgcgcaccg atcgccttca gctcgcgcat
    25441 cagcgccagc acgtccggat aggtcagcgt gaagcgttcg acatcgagca ccggatcggc
    25501 gagccgggcg cgtaccagcg catcgccgac atcgtgcatg tcggtgaaat cattgacgtg
    25561 cggccggtca tcgaccgccg cccacgccgc gcgcagctcg cgcagcgtgt ccggcccgag
    25621 cgtcgtgaac aggaacagcc cgcccgggcg cagcacgcgg cgcacctcgg cgaacacggc
    25681 atcgggcgcg ccgcaccact gcagcgtgaa gttcgaccag accagatcga ggctgtcatc
    25741 ggcaaacggc aggcgcgcgg catcggcgca gaccgcgcgc aggcgccgga accagccgga
    25801 gcgggcgcgc gtgcgggcgc acatcgccgg agccagatcc gcgccgcaga ccagcgcctg
    25861 cggatagcga tcgagcagcg cacgcgtgtg caggccggtg ccgcagccga gatcgagcac
    25921 gcgcgccggc acgagcttca cgagatcgag ccgctccagc aggcgcgtgc cgacctcgcg
    25981 ctgcagcacc gcacagtcgt cgtagcgcgc ggcagcggca tcgaaggcgg cacgcaccag
    26041 acgggcgtcg gggcgggtgg cgggcggcag cggatcgttc atcggggctc gggcgccggg
    26101 caggccggct gtcagtcgtg cagcgccgtc gccagcgccg catggaattc gtcgggatgc
    26161 gacaggaagg gcgcgtgcgt cgcgtgccgg aacacctgca cgcgcacgtc cgccgccagc
    26221 gccgtcagcg cctcgccggc cgcgaccggc accagcgcgt cgcgctggcc gagcagcagg
    26281 cgcaccggac agccgagccg cggccactcg gcgcgcaggt cggcggcttc gaggatcgaa
    26341 agcccgtcct cgagcgcgac cgtcgccggc aggccgtggc ggaacacgat ctcgcgcagc
    26401 acgcgcaggt gcgcccgggc attcggcagg ttcagggttt cgagcgcgat gaagcgctcc
    26461 agcgtcgcct tcgggtcgtc ggcaagacca ctggcgaact tcgcgagcgc ggccgtcgtc
    26521 atcgcgcacg gccagtccgg gcgctgcacg aagcaggcgt tcgagccggt gagcagcagc
    26581 cggtccacgc gcgccggcgc atcgagcgcg atgcgctgcg ccaccagccc gcccatcgac
    26641 cagccgagcc acgcggcgcg ggccggcgcc accgccgcca ccgccgccgc cagaccggcg
    26701 agcgtgccgg gccggccgcc ggagcggccg tgaccgggca ggtcgatgac ggtcacgcgc
    26761 cagtcatcga tgaaggcctc gaccgtgtcg tcccagacgc cgccgtgcat gccccagccg
    26821 tgcagcagca ccagatccgg ccccgaaccc cagctttggc agtacagcat cgcctgcggc
    26881 ctccgcgact caggccggcg ccggcagcgc cgcggccagc gcgtcgagca ggcgatcgag
    26941 ctgcgcatcg gtgtgcgcgg cgctcagcgt cacgcgcagc cgcgccgagc cggccggcac
    27001 ggtcggcggc cggatcgccg tgatccagat gccgcgatcg gcgaggcgct gcgcagcggc
    27061 gagcgcatct tcggcggccc cgatgacgat cggctggatc ggggtcgtcg agggcagcag
    27121 ccgcgcgccg aaaccgagct gcgcggcgcc ggcccggaag cgcgcgatca gcgccgtcag
    27181 ccgttcgcgc cgccagtcct cggcctgcac gatcctgaga ctggcgcgcg tcgccgccgc
    27241 cagcgccggc gaggacgccg tcgtataaat gtagggccgc gccttctgga tcaggtattc
    27301 gatcagttcg gcgctgccgg cgacgaaggc gccgctggtg ccgaaagcct tgccgagcgt
    27361 gccgatcacg accggcaccg cctcggcatc cagcccgtgc agcgccagac tgccgcgccc
    27421 ctgcggcccg agcgcgccga agccgtgcgc atcatcgacc accagccagg ccgcatgccg
    27481 gcggcaggca tcggcgagcg ccggcagcgg cgcgacatcg ccatccatgc tgaacacacc
    27541 gtcggtgacg acgatgcgct cgccgccggc cacggcgagc ttcgcttcga gcgcctccgc
    27601 gtcggcgtgc gcgtagcggc tcagccgcgc accggagagc cgcccgccgt cgagcagcga
    27661 ggcgtggttc agccggtctt cgagcacgct gtcgccacgc ccggccagcg ccgcgagcac
    27721 gccgaggttg gcgagatagc cggtcgagaa cagcagcgca cgcggccggc cggtgaaggc
    27781 ggcgagttcc tcctccagcg ccgcatgctc ggccgaatgc ccgcagacca gatgcgccgc
    27841 gccactgccg cagccccggc gcgccgcggc cgtggccagc gcctgcgcga tgcgggcatc
    27901 ggcggcgagg ccgagataat cgttgctgca gaagttcagc cactcgcgcc cgccggcggc
    27961 gatcaccggc ccctgcgcgg aatcgaccgt gcgcggcgcc cggtacagcc cggcggcgcg
    28021 cagcgcggcg agttcggcgg cgaggtcacg catcggccgg cggcagggct caggccccgg
    28081 cgcgcagctt cgccgcgccg cagccgcacg gcgcttcctg cacgaagtcg ccggcctcgg
    28141 gcagggtttc ctggatttcg agcgcgcgca cgccgagctt cgcgaacagc gcgcggtcgc
    28201 ggtcggtgtc cgggttggcg gtggtcagca gcttctcgcc gtagaagatc gagttggcgc
    28261 cggccgagaa gcacagcgcc tgcagttcgt cgctcatgtc ggtgcgaccg gccgacaggc
    28321 gcacgaagga ctgcggcatc agcagccgcg cgaccgcgat cgtgcgcacg aagtcgatgc
    28381 cgtcgagcct gtcgcggccg tacagcggcg tgccctcgac ctgcacgagc aggttgatcg
    28441 gcacgctctc cgggtgctcg gggaggttgg caagctgcac cagcatgccg gcgcgatcgc
    28501 gcagcgattc gcccatgccg aggatgccgc ccgagcagac cttgatgccg gccgagcgca
    28561 cgtggccgag cgtgtcgagg cggtcctgat aggtgcgcgt cgtgatgatc gagccgtaga
    28621 actccggcga ggtgtcgagg ttgtggttgt agtagtcgag gccggcgtcc ttcaggcgct
    28681 gcgcctgatc ggcgcgcagc atgccgagcg tcacgcaggt ttcgagcccg agcgccttga
    28741 cgccctcgac catcgccacg accgcatcga gatcgcggtc cttcggattg cgccaggccg
    28801 cgcccatgca gaagcggctg gcaccgctgg ccttcgcggt gcgcgccgcc gacaggactt
    28861 cctcgaccgc cagcagcttc tcgcgctcga cctcgccctc gtggcgcgcg ctctgcgggc
    28921 agtaggcgca gtcctcgggg caggcaccgg tcttgatcga cag
//
LOCUS       DAOQIE010000010        28688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_696_length_28688_cov_7.780184, whole genome shotgun sequence.
ACCESSION   DAOQIE010000010 DAOQIE010000000
VERSION     DAOQIE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 28688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28688
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_28688_cov_7.780184"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..840)
                     /locus_tag="PK144_01295"
     CDS             complement(19..840)
                     /locus_tag="PK144_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64386.1"
                     /translation="MNPDIHEFIHPHHLAVFMAAAREFNCHILIRKTGRASIEWVGKS
                     GYTGKRGDLKAKTANLEVAGHAVAGLVCSPLLQPLAFTEDRLASARKEWMKCSHLITE
                     PANGFDDDRPPQGCPTPYILQTKRNHRHYGCVALVDMGLLTPRYVHGDYDLYAIIPAN
                     QPFRPETIQPRHLTMGSTMTPASQTLMERLRLQSPNFEGPLSFQISNYVNTRISGLGV
                     DLLCALMVNHGEQVNIGEPGCTFEPVLAIMPAPRDGSWTIILGNRAEHERFYQNA"
     gene            complement(911..4186)
                     /locus_tag="PK144_01300"
     CDS             complement(911..4186)
                     /locus_tag="PK144_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64387.1"
                     /translation="MSDPVTSAAVAAAKFVGEKAISYLAMKVYEGSPCASPEQLTAIP
                     KLFCMAVRGAAGVDEKEYRQHLTQQIDLIAECLGRVEQAVSSINQNVTRLLQEMNLVH
                     LEIAEALVGQSAFSALQQIRSLYRNYSGRIASAGSGADELTVFFKDILQTSRLHLQTA
                     VIVDALVNPFAGKPGLLQNVLGQIAIKGSGKGLAACYELYETYTRALVLDLLKGQQLV
                     EAAIGYLQILAEERKLPELKAGLPYSAAGWQAECERQRQSVADAFHLQLDGFVLKRDL
                     DKASGLNPYFLSSEARGVFMQADLFSARLCQRYGIHGRIFSMGDRFDGTLKLDADTFP
                     ARARATIPLDEPLDYWSATTPGVYDCVAFANAFVVHRYRIPLTQAGTRKITSALPYSP
                     ADIETVDLGQAATGWFEETPAAGSAPAIFGSFIEMARAGGSFAFLSGEWEQGRRDQDS
                     SNPRTDGNDSYPQGVAQVAELHRMEVTSLREDRRMLGSVRQPEVGLSKGGKVIHALSA
                     RGDPDFHPELPEGRDQSHWRGAYATWWQTRKTITLPMPPEGQTLELHAALTPQLQADS
                     ALADAATLNGWLKGSWGRPLQPSSSISDAELLGKPVIVGVNYDPSLAFPDQGCSLTAG
                     ISLYPVFAEGPASHKDSPHTGTDDHAIALGNASCLPKYRGVFREQASTQPRQLRAFLS
                     DLYQPYGTRLKFRFEAYYRFAIETSGLDDTPFAMYARGRLENACFRLAPAGNALPPGI
                     YFDLKDPADLGFAYDYDGTGRPDHLVLYRPGSGRILISRHLGSNEFRPVGPRGPEGIG
                     IGGFDLKVAGDRIFPFDHAGSGHADHLLAYRPGNGAVSILKRNGGVFEAVFSEPVGGK
                     GIGGFNLDNPADQGFAFDYDSSGKLDHLVFYRPGSGALSILKHDDKGFSPVYAQGASG
                     KGIGGFDLSGAQDKVFAYDHDGRGRMDHLVLYRPGAGRISILKRQGSSFVAVYQRDSG
                     GIGGISGYDLANPADLGFAFDFDGKGSNDHLVFYRPGKGAIFILKPSDGDFKAVYRQG
                     DGGGGGGIGQYDLRHPADRVFPFDYRGNGLADHLVLYRPGTGVLQIIRNNSGAFVRIY
                     AKP"
     gene            complement(4677..5096)
                     /locus_tag="PK144_01305"
     CDS             complement(4677..5096)
                     /locus_tag="PK144_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNF64388.1"
                     /translation="MADDVVLNKAASIERCVARAREEYAANPAGFAHDYTRQDAAILN
                     IQRACEAALDMGQHVIRRERLGIPQSARDVFTLLARAGWIDAGLAESLRRMVGFRNIA
                     VHEYQTLQMPVTIAIITQHLDDFLDYSRRMLQRDANG"
     gene            complement(5086..5481)
                     /locus_tag="PK144_01310"
     CDS             complement(5086..5481)
                     /locus_tag="PK144_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF64389.1"
                     /translation="MDPDTLLHRVRERLPALLAFYAFGSRIDGTAGPDSDLDLAVLVA
                     GYADPLELWTLSGELADLAGCPVDLLDLRAASTVMQYRILTTGRCCWARDASVAIYEA
                     GILSDKTELDTARAGLLADIVERGSIHGR"
     gene            complement(5515..6375)
                     /gene="speE"
                     /locus_tag="PK144_01315"
     CDS             complement(5515..6375)
                     /gene="speE"
                     /locus_tag="PK144_01315"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNF64390.1"
                     /translation="MTLDTSWFSDVFADAPSAFSLKVTRKLHEEQSPFQKVEVYETEG
                     WGRMLAIDGIIMLTERENFLYHEMLAHPALYAHPAPERVLIIGGGDCGTLREVLKHPG
                     IERVVQCDIDEAVTRAAVQFFPRLTEANDDPRAELVFEDGVKYVADSADGAWDLILVD
                     STDPFGPSTGLFSEPFLREVVRALAADGLYVQQSESPIFQLDECILPMHRNLRAAGFA
                     EATELHFPQPVYQSGWWSAAFAARQPGALARRDDARAAVACDYYNADMHRAALAQPEF
                     VRRALATVWG"
     gene            6527..7216
                     /locus_tag="PK144_01320"
     CDS             6527..7216
                     /locus_tag="PK144_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64391.1"
                     /translation="MNLRVRALALPSLALLLSACTTYVPVPYRPMADAGVSPGACGNF
                     EADSRYAPLRGRVALDNPAQQTETMLRDPGRPSRAERAALGEWRRASDQCRRTTIAAQ
                     RDAGVPEAQLALDTRLADDLDALSGQLAAGRISYGEFARQRQSLADGYNSASARVAGS
                     GEGYAPRYYAAPPVYYSAPAPVYVAPPAYYYPPPYYYRPWYPAIGGSVWYGSGWHGRH
                     GHGGWGVGIGF"
     gene            complement(7246..7704)
                     /locus_tag="PK144_01325"
     CDS             complement(7246..7704)
                     /locus_tag="PK144_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNF64392.1"
                     /translation="MPDRELRCLLIGFAGGRLLLPGSMVVEVLPYATPLRVAEVPRWV
                     LGSLLWRARTVPLLAIEPLVTGQPAAPGPRSRIVVLTTPSERVQHLALVSLEVPQLSL
                     LKREMLKASDEVDCPPGMLRAVRIGGDQDAFIPDFDLLCEELAPLGIYRA"
     gene            complement(7877..13423)
                     /locus_tag="PK144_01330"
     CDS             complement(7877..13423)
                     /locus_tag="PK144_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64393.1"
                     /translation="MTARYADTPPDSVTAELEATLERVKTALEIHCEDPDAPGPLDDI
                     VRGLRDVRGACVLLEQHEAAQQAGELLGWAERLMHGQTEDAEGLRARLLESVLRLARY
                     LAWRHRSRHRAEAAPPAPVIDLTAFALDLNTLRSRFQRTLLGLLRGDKLDHHLDQLAT
                     VLMDIKALCPAGADYDLWWHAQGLAESLRSDGRAPSRTQNLLLRELDQRLRLLAQHGP
                     GALPPLVLAELLGRLELEVSASPVGKAWLRNRIDYTPGASGTEPGARADVLADPDTLS
                     TIGLQLAEQLALAKGALETRTRTGVADAPSLQEELSGLRSVANLLWLIDMPAPAALLR
                     RWLERLDELAARNASLDDDDLFGLAHDVVLVENSVEGITEFAYAPPPAVGDDPLAAIA
                     ALEVRRARAATANAAGELLQDARTQIDARLRGEGSDELWPAIARLIDHVASVLRMLEA
                     EPAVQLVKQIRYAIGRVEADPHRLDAPAFAAAVAEAIVVAEYFVDRLAQGRPTDEDVL
                     ERARVDLIGACGGPVPDWDLPERWQAAGNEAPADTTTPDFELTLPALELPPASAAGDT
                     GKADQPDRTGETARPTVVFLGDDALPPLDLDSLQLEMPGPATGRPTPDAIELIEPVEP
                     ARPAATPAGLAATDAPAGETALPSVEFTDFDDLGDLAAFGLKPDGSSVHAPADDSDTF
                     APPQLPADLWPQTPPAPARHAAGERALSWPDDLPELAIELPAAAPPAPVADEAAADEP
                     ADAALPLVSFTIPEPVPAPEPAGAATPAVPTPPPESALPLPAAALDIDPDLLDVFLEE
                     AAGEFESIRAHLAQTEGAAPGELEGLRSIRRSFHTLKGSGRMVGERVVGEFAWGLENL
                     FNQILAGRRPFNAEVQALTAEGTDALEHWLASRDAAAYGRLTVLELRTHALLEGRAPS
                     EADVDLEAETPAAAETLTPAPAPEPAPAPAVTETVAPAPLPPPLPDWVEALLLVLDDC
                     RAGSTTARDALIERLAVIVAPLASAEAVRVARALEDYLQALCRHGRGLDAADILLCED
                     CASALQAYALGETIDPGALGELLNALARRAAALPSFAAPLPAEPQPVLPAIDPELAAV
                     FLAEAADILDAADVTLSRWRQDRHSAELLNDLRREMHTLKGSSRMAGFPQIGDLAHAT
                     ESLLDACAGGKSEPTDALADGMQRALDHFSTMLTEVEANRAPAPAAELIHDLADLLEH
                     GAPVPAPAPAAATAATATTAKPASPAGTAESVRVSSTLLDNLVNQMGESSIYRARIEQ
                     GVSAVRFNLGELDQIANRLRAQLRRLEIETEAQILFRYERGGVDPNADFDPLELDRFS
                     ELQQLSRQLLEAAEDLANIQSSLVDQTGEIGGLLEQQGKVNKELQQGLMQTRVVRFET
                     VAARLKRVVRQAADDAGKSAELHIDGGDAEIERNLLEGLVAPLEHLLRNAVSHGIESP
                     ERRHAAGKDATGAIRLALRREGAEILLSVADDGAGLNYAAIRASAEKRGLLAANAQVS
                     DAQLADLLLLPGFSTAQALTQLSGRGVGLDVVASAVSSMRGALSIQSQPGKGVTFQLR
                     LPFSLSLTQALLVRAGEDIYAVPLLSVEAVSRLSPREFRDYLAGHTAEHDYAGSGFAV
                     HSLAAVFGGALPAAGSSEARPPVLLFRSADMRAAIQVDAVLGRQEIIVKPVGPQLSAV
                     PGVSGATVLADGRVVIILDMPALVRGLASAARRAQEAAALRAARQADERAPLSILVVD
                     DSITMRKVTARVLERHGVVVELAKDGVDAVEVLDQLVPDLVILDIEMPRMDGFELVGH
                     IRNQARLRHLPVIMVTSRTGEKHRDRAARLGVNAYLGKPYQEEELLGSIRDVLGSEGA
                     RRLRSL"
     gene            complement(13459..15528)
                     /locus_tag="PK144_01335"
     CDS             complement(13459..15528)
                     /locus_tag="PK144_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF64394.1"
                     /translation="MSEPRNTTARPGGAPRFALYGVALVVSVLLMAGTFFLVQRHETG
                     ITADLALVNEQRLLARTLTALAVEAVQGQDDAFDRLGQARTRFDSSIAALRNGDATTA
                     PVPTEAGDALAQLEKRWQETRGAVSAVLESREPVKAVRALLPVFEETLPALHDYADDL
                     SGGLADGGAPGAQVRAAARLSTLAQRMQYDLGLLVRGDANSALAADRLLRDVSQFGRL
                     AKALREGDPRQGLTPMNAPALLPKLNDITAGAAQLEKSIGQMLAYAPELFQAQDATQR
                     LVNGSAPLAQAAAALGEALTSRADSVHIAGIPVGFGFVYFFAALSLMLLMLLLRKQVV
                     EARVRLEESEQRKQQTEDQNRRNQEAILRLLDEMGDLAEGDLTVQASVTEDITGAIAD
                     SINYAIEALRDLVTTINDTSGRVAAAAEETRSIAERLASASQEQASQIETSGNTIAQM
                     ARSMDEVSSKTASSSEVAQQAAALAREGGERVRRTIQGMDAIREHIQETSKRIKRLGE
                     SSQEIGDIVELINDIADQTNILALNAAIQASAAGEAGRGFAVVADEVQRLAERSGNAT
                     KRIESLVKTIQADTNEAIISMEKSTTEVVNGAGLAETAGVSLNEIESVSSRLAELIGD
                     ISSAATEVSTMAARVAGMMGSINAITAETAEGSADTARAIGDLTSLAQELRQSVAGFR
                     LPEQDAA"
     gene            complement(15610..16143)
                     /locus_tag="PK144_01340"
     CDS             complement(15610..16143)
                     /locus_tag="PK144_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNF64395.1"
                     /translation="MAAAQHPFDQLLELAGRSGQGSAAQAASPTSSGALGLRVGAHAL
                     LFALAETGEIVPVPRLTRVPGVQPWLLGIVSLRGRIVPVIDLGDFLEGRHTSIGPRTR
                     IVIASGGDWSYGLVVDEIIGMRPIGPANRLPELPAGTAPTLKPYARAAYAIDNQVWSE
                     FDIGRLLADPRFMAAAL"
     gene            complement(16156..16527)
                     /locus_tag="PK144_01345"
     CDS             complement(16156..16527)
                     /locus_tag="PK144_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64396.1"
                     /translation="MARILIIDDSPTEIFALTRILESRGHQVASAGNAEEGIARAQAD
                     HPDLILMDVVMPGMNGFQATRKLAKTPSTAHIPIIMVTTKDQETDRIWGLRQGAQEYV
                     VKPPVEAELIAKIDTLLGKRT"
     gene            complement(16593..17021)
                     /locus_tag="PK144_01350"
     CDS             complement(16593..17021)
                     /locus_tag="PK144_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64397.1"
                     /translation="MRASRNGGDSSVEAGGANSAIKVMVVDDSRTIRRTAETLLEREG
                     FTVITADDGFEALSRMVESEPDIVFLDIMMPRLDGYQTCSVIKSNGRFRATPVIMLSS
                     KDGIFDKARGKLAGSEQFITKPFTREDLLEAIQQHVHVSK"
     gene            17224..18171
                     /gene="gshB"
                     /locus_tag="PK144_01355"
     CDS             17224..18171
                     /gene="gshB"
                     /locus_tag="PK144_01355"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNF64398.1"
                     /translation="MNKRLGVVMDPIATITIKKDTTFAILLAAQARGWSIRYFEQSDL
                     YLRDGRACGRARALTVRDDRQDWYTLGEPQDEPLAGLDVLLMRKDPPFDLEYIYTTYL
                     LERAEAEGVRVVNRPQSLRDANEKLFTAWFPQCCPPTLATRDMARLRGFVAEHGDAIL
                     KPLDGMGGAGIFRVRAGDANLNVIVETLTERGRRTALAQRFIPEISAGDKRILMIDGE
                     PVPYALARIPAEGETRGNLAAGGRGVGQPLSERDRWICAQVGPELRRRGLVFVGLDVI
                     GDWLTEINVTSPTCARELDAQFGLDIGADLMRALEATAA"
     gene            18168..19232
                     /locus_tag="PK144_01360"
     CDS             18168..19232
                     /locus_tag="PK144_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNF64399.1"
                     /translation="MRLFSFAGRRRVAALLGCVFALLLAACEKPDPLQATRLLVFGTV
                     VDVATWGVTPKPAQAAFDELRTAFESWHREWHAWEDSPLSRANRAFAAGAGAALTPDL
                     LRLVSDARAWSQASNGLFDPAIGRLVALWGFNQGERPDAPPPPPQAIAELVNAHPSMD
                     DLTVDGEQLRSRNPQLWLDFGGIAKGYAVDRAIERLRARGVQNAIVNAGGNLRAIGSK
                     DGQPWRIGVRDPRGPGVLASLTITGDESVSTSGDYERHFEWQGVRYHHVLDPRSGYPA
                     IGAVAVTVVAESAEKADVASTVLMIAGPTEWRALARALGVTQVLRIDESGQVELTPAL
                     AARLHWEVSPPPTLTVVPEP"
     gene            19229..19633
                     /locus_tag="PK144_01365"
     CDS             19229..19633
                     /locus_tag="PK144_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNF64400.1"
                     /translation="MSPPAPALHPALRGRLTAGDAVVLALSLAGLVALAVQLWGADAR
                     PDTALVRIDGREVLRLSLAEAGQYAVEGPAGTTRVEVRDGRIRCVSSPGPQHLCERAG
                     WLSHAGEVAVGLPNRVSIEVLGRNRRFDSISY"
     gene            19657..20199
                     /locus_tag="PK144_01370"
     CDS             19657..20199
                     /locus_tag="PK144_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNF64401.1"
                     /translation="MPAARPPLEPLTLAVDAADRRIAWYAALALALHVLEAALPSPLP
                     GLKPGLANVLVLIVLVRHGLAAAAWVAGLRVLVGSLLVGTFLSPAFLLSAGGALAALL
                     VLALAHASRCFGPVGLSLLAAEAHMAMQFALARLLLVPHAALWQLLPLLMTAAVATGL
                     AGGIIARALLRTDAAAGSRP"
     gene            20196..21113
                     /locus_tag="PK144_01375"
     CDS             20196..21113
                     /locus_tag="PK144_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF64402.1"
                     /translation="MNAILYAHLLQERPSRRLPLALLFALGVHVALLAGIGLGRPAVP
                     FDPVLSFKVSLVERAGHAERDAPLRAPEPQPLPPVPAEPPPAVMAEPEPEPEPQASPV
                     PAPPVISTQAPPAPARPVVAAPRQVEPKPVEPKPVEPRPVEPKPVPAPRPVAPPAPRR
                     SGPLSAAELMGAGLDSIRQEAPLPAASSRGRSKRADPTDSSTLEGYYAAAWVAKVERI
                     GALNFPEEARRRNLTGSLRLSVVIRADGTILETTVVRSSGQSALDEGARRIVELGAPY
                     APFPDELRRRYDTLVIVRDWQFRQGAQFQ"
     gene            21184..21744
                     /locus_tag="PK144_01380"
     CDS             21184..21744
                     /locus_tag="PK144_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNF64403.1"
                     /translation="MLEPSYLSHQFLIAMPALADPNFARTVTYLCEHGPQGALGLIVN
                     RPLDLTTADLLENLGIEAPPGTGALPVYFGGPVHPEQGFVLHRPLGRWQSTLATGDGL
                     AITTSRDILEAMARGQGPAEALIALGYAGWGPGQLERELAENAWLSCPADADVIFRLP
                     AAERWAAAAALLGVDLRLLCGDAGHA"
     gene            21746..22201
                     /gene="ruvX"
                     /locus_tag="PK144_01385"
     CDS             21746..22201
                     /gene="ruvX"
                     /locus_tag="PK144_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNF64404.1"
                     /translation="MPASAPRPRTVLAFDYGSVRIGVAVGSELTGSARPLATLAGRDW
                     TAIGRLIDEWRPDACVVGVPRHADGSESETTRGALRFARQLDGRYGLPVATHDERLSS
                     YAAAGRLAAAPASRRRPGPGLDAAAAALILESWFATVRNDPPCNSTPNA"
     gene            22177..22650
                     /gene="pyrR"
                     /locus_tag="PK144_01390"
     CDS             22177..22650
                     /gene="pyrR"
                     /locus_tag="PK144_01390"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNF64405.1"
                     /translation="MQLDAERLIDTLASGLRAHLARRGIGTPLLFGIRTGGVGLAERL
                     RVALGVAEPIGTLDISFHRDDDVRDGLDPQVRPSRIPVDPEGRDLVLVDDVLYTGRTV
                     RAALNELFDYGRPRSVTLAVLVDRGGRELPIQADVVAELVTAAPGERVELRWPGG"
     gene            22803..23789
                     /locus_tag="PK144_01395"
     CDS             22803..23789
                     /locus_tag="PK144_01395"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNF64406.1"
                     /translation="MAMTPSSIQLDADGRLRHFLTVEGLKRRHLVEILDRAEALSGVA
                     ERRMTKSPTLHGKTVVNLFFENSTRTRTTFELAAKRLSADVLNVNISTSSTHKGETLL
                     DTIRNIEAMQVDMFVVRHAVSGAAEFIARHVAPHIAVLNAGDGRHAHPTQAMLDMMTI
                     RRHKGEFANLRVAIVGDVLHSRVARSDIHALNILGTGEVRVVAPKTLLPADVGTLGVH
                     VFHDLEHGLRGVDVVIMLRLQRERMSGALLPSASEYFQQYGLTAERLALAQPDAIVMH
                     PGPINRGVEMDSAVVDGPRSVILEQVTHGIAVRMAVMAIVLGNRHPDITEET"
     gene            23794..25074
                     /locus_tag="PK144_01400"
     CDS             23794..25074
                     /locus_tag="PK144_01400"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF64407.1"
                     /translation="MRITLQGGRVIDPAHGLDAVTDLHIADGRIAGLGAAPAGFIAER
                     SCDVSGRIVCPGLVDLCARLREPGAEHKATIASETHAAAASGITTLVMPPDTDPVIDE
                     TAVVESIRRRAQAAGFARVLVLGALTRGLGGEVLAEMAALKEAGCVGVGNAGVPVPNS
                     MVMKRAMEYASSHGLTVFLTPMDPWLSAGGQAHDGQVAARLGLAGIPVAAETAAVGRE
                     LALVEETGARAHFGRLSSARAVAMIARARDRGLPVSCDVAAHQLHLTEVDLGRFDADA
                     HVLPPLRTARDRDALRRGVAAGVIEAVCSDHQPHEPDAKLVPFGDTEPGLSALDTLLG
                     LMLKLVDEGVLDLPGAIERVTAGPARLLGIEAGTLAVGTPADVCVFAPGAHRLLDART
                     LASRGHNTPFLGWELRGVVTLTLLDGRPVYQREV"
     gene            25080..25769
                     /locus_tag="PK144_01405"
     CDS             25080..25769
                     /locus_tag="PK144_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64408.1"
                     /translation="MDTSRSVQRSGCTELPGGWRVERWQAPALVDHARPGQRLAAGSA
                     ELPVMRTRAATGELECLARGPAGAAVAAADADLRGEPFELSAPSPRALLIGDLSGLAP
                     LVFLADRLRSAAVRVKTFAILGLDGEAPFRPVPSRLIVPGVPAWVTGTLPLFEDWGIA
                     ARLASAGEDRPGCFEGTPVQLARGWLAAQQGVRDVCVYACAGPALLEDTRALAAAFGL
                     GYQGRAAGSAC"
     gene            25780..>28688
                     /locus_tag="PK144_01410"
     CDS             25780..>28688
                     /locus_tag="PK144_01410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF64409.1"
                     /translation="MSAPSGDDGAGRRHPAQLAALLQRQAPTLLLAVLTFVAIGLLSG
                     ATLWQIDNDRVRTLEDARRANVQLADALAEHGSRLIESVDVLLRDFSESPPAPGTGAL
                     GGDFREAYAQSLLFLRNLALLDADGRMYATLYYSEMRGRSFAGRDYYLLHRAQADAGL
                     VVSTPKVSPADGRSRILTLSRRLNDAGGAFAGVVVASVGLDTLERYYRSIERAPGQGI
                     ELRLRDGEAMLSVGVEHNAGPDALEALRWLAGTPYGIVVSRLPVRVLADWRERTGSML
                     VAVAAADTLIAMLALLLGGRLHIGARLLGRLDDAERWQRTLLRTLPDPLVVLDRRLRV
                     REVCSAQPQPFGADIELRPGSALFEVLPPVVATRVGPAAAEALGDDRIRSVDYPLHID
                     GELHHYAARILRLAAEDGTVEGVLWLARDITDRKRIEQALFQEKERAQVTLAAIGDGV
                     LVTDEEARLEYLNPVAERLCDCRLAEVRGWPLAAVLRIEAEAGSELGSDPATQCLADN
                     RPVGPALHARLMAADGRERHVEISCAPIHDPAGRPTGTVTVMHDVSERHRLTQQIRHQ
                     AAHDPLTGLINRREFELRLDAACRIADDQNRTHALLCIDLDRFKIVNDSCGHLAGDEL
                     LRQVARLMTEHTRQRDVLARMGGDEFALLLEDCPLPKAAEIAGALVSAVGEFRFVHGT
                     QAFSIGASIGVAPVPPGNGGPQVVLSRADRACYAAKDAGRNCVSLANEDGDAASSGTR
                     HWHERLEALLDGGSQDYELHVQPASALYAGLPPYREVLLRLRDDDGRLVPPGAFLPVA
                     DRYRLAAGLDRFVLDALCRRLAEGAGGIDGLHAMNLSVATLEDGGFAGHVRTALQRHG
                     LAPARLGFEIEEAAVHGRLAQAGEQIGALRALGCRVTLDRCGSGLAAFDCLKVLRVDA
                     LKIDGSRLEAEGSHPVDTVSAEAIVRLARLLGLHTIASRIETTGQHSGAVVLGID"
BASE COUNT         3966 a        10042 c        10389 g         4291 t
ORIGIN      
        1 tcatccggcg ggcgctaatc aggcattctg gtaaaaccgc tcgtgctctg cccggttgcc
       61 gagaatgatc gtccaggagc catcccgtgg tgcaggcatg atggccagca ccggctcgaa
      121 agtgcatccc ggttcgccga tgttgacctg ctcgccatga ttgaccatca gggcacacag
      181 caaatccacg ccaaggccgc tgatgcgggt gttgacatag tttgaaatct ggaacgacaa
      241 cggaccttcg aagttgggtg actgcaggcg caggcgttcc atcagcgtct gactggccgg
      301 tgtcatggtc gagcccatcg tgaggtgacg gggctggatg gtttccggtc ggaaaggttg
      361 attggcggga atgatggcgt acagatcgta atccccgtga acataccggg gggtcagcag
      421 ccccatgtcc acgagggcaa cgcagccgta gtgacggtga ttgcgtttgg tctgcaggat
      481 gtaaggggtc gggcagccct gcggcggtcg atcgtcatcg aagccgttgg cgggttcggt
      541 gatcaggtgg ctgcacttca tccattcctt gcgcgccgac gccagacgat cttcggtgaa
      601 cgccaggggt tgcagcaggg gggaacacac gagcccggcc actgcatgac cggcgacctc
      661 cagattggcc gtcttggcct tcagatcgcc gcgcttgccg gtgtagccgg acttgccgac
      721 ccattcgatg ctggcccgcc cggtcttgcg gatcaggatg tggcaattga actcccgcgc
      781 ggcggccatg aacaccgcga ggtggtgcgg atggatgaat tcatggatgt cgggattcat
      841 gaaaggctcc gcgataaggg ttcgtaaccg gaatccgccg ccggcgtcct ccctgacgcc
      901 ggcggcatgt tcagggcttg gcgtaaatcc gcacgaaggc accgctgttg ttgcggatga
      961 tctgcagcac gccggtgccg gggcggtaca gcaccagatg atcggccaga ccgttgccgc
     1021 ggtagtcgaa cggaaacacc cggtcggccg gatggcggag atcgtattgc ccgatgccgc
     1081 cgccaccacc accatcgccc tgccggtaga ccgccttgaa gtcaccgtcc gacggtttca
     1141 ggatgaagat ggcgcctttt ccggggcgat agaacaccag gtgatcgttg ctgcccttgc
     1201 cgtcgaaatc gaaggcaaag cccagatcgg ccgggttggc gaggtcgtaa ccactgatgc
     1261 cgccgatgcc gccgctgtcg cgctggtaga cagcgacgaa actgctgccc tgacgtttca
     1321 ggatgctgat cctgccggca cccgggcgat acagcaccag atgatccatc cggcccctgc
     1381 cgtcgtggtc atacgcaaac accttgtcct gcgcgccgga aagatcaaaa ccgccgatgc
     1441 ctttcccgga cgccccctgc gcatataccg ggctgaaacc cttgtcatcg tgcttgagga
     1501 tggacagcgc accgctgccg ggccggtaga acaccagatg gtcgagcttg ccgctgctgt
     1561 cgtaatcgaa ggcaaaaccc tgatcggcgg gattgtcgag attgaagccg ccgatgccct
     1621 tgccgccgac gggttcggaa aacaccgcct cgaacacgcc gccgttgcgc ttgaggatgc
     1681 tgacggcgcc attgccgggc cggtaggcga gcaggtgatc ggcatggccg ctgccggcat
     1741 gatcgaacgg gaagatccgg tcgcccgcca ccttcagatc gaagccgccg atgccgatcc
     1801 cttcggggcc gcgcggaccg acgggccgga attcgtttga accgagatgg cggctgatca
     1861 ggatcctgcc gctgccgggg cggtacagga ccagatgatc cggccggccg gtgccgtcgt
     1921 agtcgtaggc aaagccgaga tcggcagggt ctttcaggtc gaaataaatg ccgggcggca
     1981 gcgcattgcc ggcgggggcc agccggaagc aggcattttc cagccggccg cgcgcataca
     2041 tcgcaaacgg cgtgtcatcg agaccggagg tttcgatcgc gaaacggtaa taagcttcga
     2101 agcggaattt cagccgcgtg ccgtatggct ggtacagatc gctcaggaag gcacgcaact
     2161 gccggggctg cgtcgatgcc tgttcgcgga acacgccccg gtacttcggc aggcaactgg
     2221 cattgccgag cgcaatcgca tggtcgtccg tgccggtgtg cggggaatcc ttgtggctgg
     2281 ccgggccttc ggcgaatacc ggatacaggc tgatgccggc agtcagcgag cagccctgat
     2341 ccggaaacgc cagcgacgga tcgtaattga cgccgacgat gaccggcttg ccgagcagtt
     2401 ccgcatcgct gatcgaagag gaaggctgca gcggccggcc ccagctgccc ttgagccagc
     2461 cgttgagcgt cgcggcatcg gcgagggcgc tgtcggcctg caattgcggg gtcagcgcgg
     2521 cgtgcagttc gagggtctgg ccctcgggcg gcatcggcag cgtgatggtc ttgcgcgtct
     2581 gccaccaggt cgcatacgcc ccgcgccagt ggctctgatc gcggccctcg ggcagctccg
     2641 ggtggaaatc cggatcgccg cgggcactga gggcgtgaat caccttgccg cccttggaaa
     2701 gccccacttc cggctgacgc acgctgccga gcatgcgccg gtcctcgcgc aggctcgtga
     2761 cttccatgcg gtgcagctcg gccacctggg cgacgccctg cggatagctg tcattgccgt
     2821 cggtgcgcgg gttgctgctg tcctgatcgc ggcggccctg ctcccactcg ccgctcagga
     2881 acgcgaagct gccgccggcg cgggccatct cgatgaagct gccgaagatc gccggcgcgg
     2941 aaccggccgc gggggtttcc tcgaaccagc cggtggcggc ctgcccgaga tcgacggttt
     3001 cgatgtcggc cggggaatag ggcagcgcgc tggtgatctt gcgcgtgccg gcctgcgtga
     3061 gcgggatgcg gtagcggtgg acgacgaagg cattggcaaa cgccacgcag tcgtagacgc
     3121 cgggcgtcgt ggcgctccag taatccagcg gttcatcgag cgggatcgtg gcgcgggcgc
     3181 gggcggggaa ggtatcggcg tcaagcttca gcgtgccgtc gaaccgatcg cccatgctga
     3241 agatgcgccc gtgaatgccg tagcgctggc agaggcgggc actgaagaga tcggcctgca
     3301 tgaacacgcc gcgggcttcg gacgacagga agtacggatt gagcccgctg gccttgtcca
     3361 gatcgcgttt cagcacgaaa ccgtcgagtt gcaggtgaaa ggcatcggcc acggactggc
     3421 gctggcgctc gcattccgcc tgccagccgg cggcgctgta cggcaggccg gccttgagtt
     3481 cgggcagctt tctttcctcg gcgaggatct gcagataacc gatggccgcc tccaccagtt
     3541 gctgaccctt gagcagatcg agcaccagcg cgcgggtata ggtttcgtac agctcataac
     3601 aggccgccag tcccttgccg ctgcccttga tggcaatctg cccgagcacg ttctgcagca
     3661 gcccgggctt gccggcgaac ggattgacca gcgcatcgac gatgacggcg gtctgcagat
     3721 gcaggcggct ggtctgcagg atgtccttga agaacaccgt cagttcgtcc gcgccgctgc
     3781 cggcactggc gatgcgtccg ctgtaattgc ggtacaggct gcgaatctgc tgcagcgccg
     3841 aaaaggccga ttgcccgacc agcgcctcgg caatctccag atgtacgagg ttcatttcct
     3901 gcagcagccg cgtgacgttc tggttgatgc tgctcacggc ctgttcgacg cgcccgaggc
     3961 actcggcaat cagatcgatc tgctgcgtca ggtgctggcg gtattccttc tcgtcgacgc
     4021 cggcggcgcc gcggaccgcc atgcagaaca gtttgggaat cgcggtcagc tgttcggggc
     4081 tggcgcaggg cgagccctcg tagaccttca tggccaggta actgatggct ttctcgccaa
     4141 cgaacttggc agcggcaacg gccgccgaag tgaccggatc ggacatgggc ggatccctct
     4201 gggcagccgt ggaaggatgg gtctggacag tcatgagcgg gcgactcggt tccggatcgg
     4261 aaaggaacgt gaccagccgg atcgccgggg cggcagaccg caccggaatc cgcaaagccg
     4321 ttcgcccggc tgcccgtcgc agacgcacac ctgcgcctgt ccatgcaccg cgggcgctgg
     4381 cggtgcaccg gaatccggtg ggcatgaaaa aggctgggga gaaacccggg tgtcatccgg
     4441 aatcggcagg gccgatggct ggggaccggc acggggggat tgtccggtcg ccagcgaact
     4501 gcggtgtgca ggaggaaggg gaggaaggca ggggatgacg gtctgccctg acaaaccggg
     4561 caatcacgga ccgcccggtt catgccgcag caagctagta gacggggact gactgtgcaa
     4621 tgcagcgggc gggcgcgaga gggcaatcag gtggcatcgg tcagtccgtg caccttttat
     4681 ccattcgcat cgcgttgcag catccggcgg ctgtaatcga gaaagtcatc gagatgctgc
     4741 gtgatgatgg caatcgtcac cggcatctgc agcgtctggt attcatgcac ggcgatgttg
     4801 cgaaagccga ccatgcgcct gagcgattcc gccagccccg catcgatcca gccggcacgg
     4861 gccagcagcg tgaacacgtc gcgggcgctt tgcggaatgc cgagtcgttc gcggcggatc
     4921 acatgctggc ccatgtccag cgccgcctcg caggctcgct ggatgttcag gatcgcagcg
     4981 tcctggcgcg tgtagtcatg ggcgaagccg gccgggttgg cggcgtattc ctcgcgggcg
     5041 cgcgccacgc agcgctcgat gctggcggcc ttgttcagaa ccacgtcatc ggccatggat
     5101 gctgccccgt tcgacgatat cggccagcag cccggcgcgg gccgtgtcga gttcggtctt
     5161 gtcactgagg atgccggcct cgtagatcgc caccgaggcg tcgcgcgccc agcagcagcg
     5221 gccggtggtg aggatgcggt actgcatcac cgtcgaggcg gcgcgcaggt cgagcagatc
     5281 gaccgggcag ccggccaggt cggccagctc gccggacaag gtccacagtt cgagcggatc
     5341 ggcgtaaccg gcgaccagca cggccagatc cagatcgctg tccgggccgg ccgtgccgtc
     5401 gatgcggctg ccgaaggcat agaacgccag cagcgccggc agacgctcgc gcacgcggtg
     5461 cagcagcgtg tccgggtcca tgcgcaggag gtccatgcgg gggccggcgc cgcttcagcc
     5521 ccagaccgtc gccagcgccc ggcgcacgaa ctccggctgc gccagcgccg cgcggtgcat
     5581 gtcggcgttg tagtagtcgc aggcgaccgc ggcgcgggcg tcgtcgcggc gggcgagggc
     5641 gccgggctgg cgggcggcga aggcggccga ccaccagccg gactggtaga ccggctgcgg
     5701 gaagtgcagc tcggtggcct cggcgaagcc ggcggcgcgc aggttgcggt gcatcggcag
     5761 gatgcattcg tcgagctgga agatcggcga ttccgactgc tgcacgtaca ggccgtcggc
     5821 ggcgagcgcg cgcacgacct cacgcaggaa gggttccgag aacaggccgg tcgaggggcc
     5881 gaacggatcg gtcgaatcga cgaggatcag gtcccaggcg ccgtcggccg aatcggcgac
     5941 gtacttcacg ccgtcctcga acaccagctc ggcgcgcgga tcgtcgttgg cctcggtcag
     6001 ccgcgggaag aactgcacgg cggcgcgcgt gacggcctcg tcgatgtcgc actgcacgac
     6061 gcgctcgatg ccggggtgct tcagcacctc gcgcagcgtg ccgcagtcgc cgccgccgat
     6121 gatcagcacg cgctccggcg ccgggtgcgc gtacagcgcc gggtgcgcga gcatctcgtg
     6181 gtacaggaag ttctcgcgct cggtcagcat gatgatgccg tcgatcgcga gcatccggcc
     6241 ccagccctcg gtctcgtaca cctcgacctt ctggaacggg ctctgctcct cgtgcagctt
     6301 gcgggtgacc ttcagcgaga acgccgacgg ggcatcggcg aagacgtccg agaaccagct
     6361 cgtgtcgagc gtcatgcagt cttaactcct gcccggcgag gccggcgcac tatcggggat
     6421 caggatgagg atcaggatca ggatgaggat cgggggcgcg cattctagcc tccgtgcatg
     6481 accatccgcc gcgggaccgg tccacccgtc gaagaggaat gccgccatga accttcgcgt
     6541 tcgcgcgctc gcgctgccgt cgctggcgct gctgctgtcc gcctgcacca cctacgtgcc
     6601 ggtgccgtac cggccgatgg ccgatgccgg ggtttcgccc ggcgcctgtg gcaacttcga
     6661 ggccgacagc cgctacgcgc cgctgcgcgg ccgggtcgca ctcgacaatc cggcgcagca
     6721 gaccgagacg atgctgcgcg atccgggccg gccgagccgc gcggagcgtg ccgcgctcgg
     6781 cgagtggcga cgcgcgagcg atcagtgccg gcgcacgacg atcgccgcgc agcgcgatgc
     6841 cggtgtgccc gaggcgcagc tcgcgctcga cacccggctc gccgatgacc tcgacgcgct
     6901 gagcggccag ctcgccgccg ggcgcatcag ctacggcgag ttcgcgcgcc agcgccagtc
     6961 gctcgccgat ggctacaaca gcgccagtgc gcgcgtcgcc ggcagcggcg aaggctatgc
     7021 gccgcgctac tacgcggccc cgccggtcta ttacagcgcg ccggcgccgg tctacgtggc
     7081 gccgccggcg tattactacc cgccgccgta ctactaccgg ccctggtatc cggcgatcgg
     7141 cggcagcgtc tggtacggca gcggctggca cggccgccac ggccacggcg gctggggcgt
     7201 cgggatcggc ttctgagggc cgggtcccgg tgccggaggc tccgctcagg cgcggtagat
     7261 gccgagcggc gcgagttcct cgcacagcag gtcgaagtcg gggatgaagg catcctgatc
     7321 gccgccgatg cgcacggcac gcagcatgcc gggcgggcag tcgacctcgt cgctggcctt
     7381 cagcatctcg cgcttgagca gcgacagctg cggcacttcg agcgacacca gcgcgaggtg
     7441 ctgcacgcgc tcgctcggcg tggtcagcac gacgatgcgc gagcgcgggc cgggcgcggc
     7501 cggctggccg gtgaccagcg gctcgatcgc gagcagcggc acggtgcgcg cgcgccagag
     7561 cagcgagccg agcacccagc gcggcacttc ggcaacgcgc agcggggtcg cgtacggcag
     7621 tacctcgacg accatcgagc cgggcagcag caggcgtccg ccggcgaagc cgatgagcag
     7681 gcagcgcagt tcgcgatcgg gcatcacgca gtctccgtgc aggcgccgcc ggcgcccgga
     7741 tcagggctca cagggggccg ggttcttcgc tcatgccggt cacagccgaa cgcccggtct
     7801 tttcagcccc ggtcccctcg tggggacggg gccggggaga gagggggatc aacaacgcat
     7861 tcactcgtga gcggcatcac aggctgcgca gccggcgcgc gccctcgctg ccgagcacgt
     7921 cgcggatcga gccgagcagt tcctcttcct gatagggctt gccgaggtag gcgttgacgc
     7981 cgagccgcgc ggcgcggtcg cgatgcttct cgccggtgcg cgaggtgacc atgatcaccg
     8041 gcaggtggcg cagccgcgcc tggttgcgga tgtggccgac cagctcgaag ccgtccatgc
     8101 gcggcatctc gatgtcgagg atgaccagat ccggcaccag ctgatcgagc acctcgaccg
     8161 catcgacgcc gtccttcgcc agttcgacga cgacgccgtg gcgctccagc acgcgggccg
     8221 tgaccttgcg catcgtgatc gagtcatcga cgaccaggat cgacagcggc gcacgctcgt
     8281 cggcctgacg cgccgcgcgc agtgccgcgg cttcctgcgc ccggcgggcg gccgaggcga
     8341 gaccgcgcac cagcgccggc atgtcgagga tgatgacgac gcggccgtcg gcgagcacgg
     8401 tcgcgcccga gacgccgggc accgccgaca gctgcgggcc gaccggcttg acgatgattt
     8461 cctggcggcc cagcaccgca tcgacctgga tcgccgcgcg catgtccgcc gagcggaaca
     8521 gcagcaccgg cgggcgcgcc tcgctgctgc cggccgccgg cagggcgccg ccgaacacgg
     8581 ccgcgaggct gtgcaccgcg aagccggaac cggcgtagtc gtgctcggcg gtgtgcccgg
     8641 cgaggtagtc gcggaactcg cgcggactga ggcggctcac cgcctcgacc gacagcagcg
     8701 gcaccgcgta gatgtcctcg ccggcgcgca ccagcagcgc ctgcgtcagc gacagcgaga
     8761 acggcaggcg cagctggaag gtgacgccct tgcccggctg cgactggatc gacagcgcgc
     8821 cgcgcatcga cgacaccgcg ctcgcgacca cgtcgaggcc gacgccgcgg cccgagagct
     8881 gggtcagcgc ctgcgcggtc gagaagcccg gcagcagcag caggtcggcg agctgcgcat
     8941 cggacacctg cgcattggcg gcgagcaggc cgcgcttctc ggccgaggcg cggatggccg
     9001 cgtagttgag gccggcgccg tcgtcggcca ccgacagcag gatctcggcg ccctcgcggc
     9061 gcagcgccag ccggatggca ccggtcgcgt ccttgccggc cgcgtgccgg cgctcgggcg
     9121 actcgatgcc gtgcgacacg gcgttgcgca gcaggtgttc gagcggcgcg accagacctt
     9181 cgagcaggtt gcgctcgatt tcggcatcgc cgccgtcgat gtgcagctcg gcggacttgc
     9241 cggcatcgtc ggccgcctgg cgcacgacgc gcttgaggcg cgccgcgacc gtctcgaagc
     9301 gcacgacgcg ggtctgcatc agaccctgct gcagctcctt gttgaccttg ccctgctgtt
     9361 cgagcaggcc gccgatctcg ccggtctggt cgaccagcga cgactggatg ttggcgaggt
     9421 cctcggccgc ctccagcagc tggcgcgaca gctgctgcag ttccgagaag cggtcgagtt
     9481 cgagcggatc gaagtcggcg ttcggatcga cgccgccgcg ctcgtagcgg aacaggatct
     9541 gcgcctcggt ctcgatctcc agccggcgca gctgcgcgcg caggcggttg gcgatctggt
     9601 cgagctcgcc gaggttgaag cgcacggccg agacgccctg ctcgatgcgc gcacggtaga
     9661 tgctgctctc gcccatctgg ttgacgaggt tgtcgagcag cgtgctggag acgcgcaccg
     9721 attcggcggt gccggccggg gacgccggtt tggcggtcgt ggcggttgcg gccgtcgccg
     9781 cggcgggcgc cggtgccggc accggggcgc cgtgttcgag caggtcggcc agatcgtgga
     9841 tcagctcggc cgccggggcc ggggcgcgat tggcctcgac ctcggtcagc atcgtgctga
     9901 agtgatcgag cgcgcgctgc atgccgtcgg cgagtgcatc ggtcggttcg gacttgccgc
     9961 cggcgcaggc atcgagcagc gattcggtcg catgcgcgag gtcgccgatc tgcgggaagc
    10021 cggccatgcg cgagctgccc ttgagcgtgt gcatctcgcg gcgcaggtcg ttcagcagct
    10081 cggcgctgtg acgatcctgc cgccagcgcg acagcgtcac gtcggcggca tcgaggatgt
    10141 cggccgcctc ggcgaggaat accgcggcca gctccggatc gatggccggc agcaccggct
    10201 gcggctcggc cggcagcggt gcggcgaacg acggcagcgc cgccgcccgg cgcgccagcg
    10261 cgttcagcag ctcgccgagc gcaccggggt cgatggtctc gccgagcgcg taggcctgca
    10321 gcgccgaggc gcagtcctcg cagagcagga tgtcggcggc atcgaggccg cgaccgtggc
    10381 ggcagagcgc ctgcagatag tcctcgagcg cgcgggcgac gcgcacggcc tcggccgagg
    10441 cgagcggcgc cacgatgacg gccagacgtt cgatcagcgc atcgcgggcg gtcgtgctgc
    10501 cggcacggca gtcgtcgagc accagcagca gcgcctcgac ccagtccggc agcggcggcg
    10561 gcagcggtgc gggtgcgacg gtttcggtga cggccggtgc cggtgccggt tcaggtgcgg
    10621 gcgcgggcgt gagggtttcg gctgcggccg gggtctcggc ttcgaggtcg acatcggcct
    10681 cggacggcgc gcggccttcg agcagcgcgt gcgtgcgcag ttcgagcacg gtcaaacggc
    10741 cgtaggcggc ggcatcgcgg ctggcgagcc agtgttcgag ggcgtcggtg ccctcggcgg
    10801 tcagcgcctg cacctcggcg ttgaacggcc ggcggccggc caggatctgg ttgaacaggt
    10861 tttcgagtcc ccatgcgaac tcgccgacca cgcgctcgcc gaccatgcgg ccgctgccct
    10921 tgagcgtgtg gaaggaacgg cggatcgagc gcaggccttc gagctcgccg ggcgcggcgc
    10981 cttcggtctg cgccagatgc gcgcggatcg actcgaactc gccggcggct tcctccagga
    11041 acacatcgag cagatccgga tcgatgtcga gcgccgccgc cggcagcggc agcgccgatt
    11101 cggggggcgg ggtcgggacg gcgggcgttg cagcgccggc cggttcgggg gcagggacgg
    11161 gctccgggat cgtgaagctg acgagcggca gcgcggcatc ggccggttca tccgctgcgg
    11221 cttcgtccgc gaccggcgcc ggcggcgcgg ctgccggcag ctcgatcgcg agttccggca
    11281 gatcatccgg ccaggacagc gcgcgttcgc cggcggcgtg ccgggccggg gccggcggtg
    11341 tctgcggcca cagatcggcc ggcagctgcg gcggcgcgaa ggtgtcgctg tcgtcggcgg
    11401 gcgcatgcac gctgctgccg tccggcttca ggccgaaggc cgcgagatcg ccgagatcgt
    11461 cgaagtccgt gaattcgacg ctgggcaggg cggtttcgcc cgccggtgcg tcggtcgccg
    11521 ccaggccggc gggggtggcg gcggggcgcg ccggttcgac gggctcgatg agttcgatgg
    11581 catccggcgt cggccggccg gtggccgggc cgggcatttc cagctgcagg ctgtcgagat
    11641 cgagcggcgg cagcgcgtcg tcgccgagga agaccaccgt cgggcgggcg gtttcgccgg
    11701 tccgatcagg ctgatcggct ttgccggtgt cgccggcggc gctcgccggc ggcagttcga
    11761 gggcgggcag cgtcagttcg aagtcgggcg tcgtcgtgtc cgccggcgcc tcgttcccgg
    11821 cggcctgcca gcgttcgggc agatcccagt ccggcaccgg accgccgcag gcgccgatca
    11881 gatcgacgcg ggcgcgctcc agcacgtcct cgtcggtcgg gcggccctgc gccagccggt
    11941 cgacgaaata ctcggcgacg acgatggcct cggcgacggc cgccgcgaat gcgggtgcgt
    12001 cgagccggtg cggatcggcc tcgacgcggc cgatcgcgta gcggatctgc ttgaccagct
    12061 gcacggccgg ctcggcctcc agcatgcgca gcacgctggc gacgtggtcg atcagccggg
    12121 cgatggccgg ccagagttcg tcgctgccct cgccgcgcag gcgggcgtcg atctgcgtgc
    12181 gcgcatcctg cagcagctcc ccggccgcat tggcggtggc ggcacgggcg cgacgcactt
    12241 cgagcgcggc gatcgccgcc agcggatcgt cgccgaccgc cggaggcggg gcgtaggcga
    12301 actcggtgat gccctcgacg ctgttctcga ccagcacgac atcgtgggcg aggccgaaca
    12361 ggtcgtcgtc atcgagcgag gcgttgcgcg cggcaagctc gtcgagccgt tcgagccagc
    12421 ggcgcagcag cgcggccggg gccggcatgt cgatcagcca cagcaggttg gcgacgctgc
    12481 gcaggccgga gagttcttcc tgcaggctcg gggcatcggc gacgccggtg cgcgtgcggg
    12541 tttcgagcgc gcccttggcg agtgcgagct gttcggcgag ctgcaggccg atcgtgctca
    12601 gcgtgtccgg atcggcgagc acgtcggcgc gcgcgccggg ttcggtgccg ctcgcgccgg
    12661 gggtgtagtc gatgcggttg cgcagccagg ccttgccgac cggcgaggcg ctgacctcca
    12721 gttcgagccg cccgagcagc tcggccagca ccagcggcgg cagggcgccg gggccgtgct
    12781 gcgcgagcag gcgcaggcgc tggtcgagtt cgcgcagcag caggttctgc gtgcggctcg
    12841 gggcgcggcc gtcgctgcgc agcgactcgg cgaggccttg cgcgtgccac cacaggtcgt
    12901 agtcggcgcc ggccgggcac agggccttga tgtccatcag cacggtggcg agctgatcga
    12961 ggtggtgatc gagcttgtcg ccgcgcagca ggccgagcag cgtgcgctgg aagcgcgaac
    13021 gcagcgtgtt caggtcgagc gcgaaggccg tgaggtcgat caccggcgcc ggcggcgcgg
    13081 cttcggcacg gtggcggctg cggtggcgcc aggcgaggta gcgcgccagg cgcagcaccg
    13141 attcgagcag ccgggcgcgc aggccctcgg catcctcggt ctggccgtgc atcaggcgct
    13201 cggcccagcc gagcagctcg ccggcctgct gggcggcctc gtgctgttcg agcagcacgc
    13261 aggccccgcg cacgtcgcgc aggccgcgga cgatgtcgtc gagcggcccg ggggcgtcgg
    13321 ggtcctcgca atggatttcg agcgcggtct tgacgcgttc gagggtcgct tccaactcgg
    13381 cggtgacgga gtcgggcggc gtgtcggcgt agcgggcagt catgcctgtc tccgtccggc
    13441 gtcgtcaggc ggcgcggctc aggccgcgtc ctgctcgggc aggcggaagc ccgccacgga
    13501 ctgacgcagc tcctgggcga gcgaggtcag gtcgccgatg gcgcgcgcgg tgtcggcgct
    13561 gccttcggcg gtctcggcag tgatggcgtt gatcgagccc atcatgccgg cgacgcgcgc
    13621 ggccatcgtc gagacctcgg tcgcggccga ggagatgtcg ccgatcagct cggcgaggcg
    13681 cgaggacacc gactcgatct cgttcagcga cacgccggcg gtctcggcga gaccggcgcc
    13741 gttgaccact tcggtcgtgc tcttctccat cgagatgatg gcttcgttgg tgtcggcctg
    13801 aatggtcttg accagcgatt cgatgcgctt cgtggcgttg ccggagcgtt cggcgaggcg
    13861 ctggacctcg tcggcgacca ccgcgaagcc gcgcccggcc tcaccggcgg cgctggcctg
    13921 gatcgcggcg ttcagcgcga ggatgttggt ctggtcggcg atgtcgttga tcagctcgac
    13981 gatgtcgccg atctcctgcg aggactcgcc gaggcgcttg atgcgcttgg aggtctcctg
    14041 gatgtgctca cggatcgcgt ccatgccctg gatcgtgcgg cgcacgcgct cgccgccctc
    14101 gcgcgccagc gccgcggcct gctgcgcgac ctcggaggaa ctggccgtct tcgaggacac
    14161 ctcgtccatc gagcgcgcca tctgcgcgat ggtgttgccg gaggtctcga tctggctcgc
    14221 ctgttcctgc gaggccgagg ccaggcgctc ggcgatcgag cgggtttcct cggcagcggc
    14281 ggcgacgcgg ccggaggtgt cgttgatggt cgtgacgaga tcgcgcagcg cctcgatcgc
    14341 gtagttgatc gagtcggcga tcgcgccggt gatgtcctcg gtgacgctcg cctgcacggt
    14401 caggtcgccc tcggcgaggt cgcccatctc gtcgagcagc cgcaggatgg cctcctggtt
    14461 gcggcggttc tggtcttcgg tctgctgctt gcgctgctcg gattcctcca ggcgcacgcg
    14521 cgcctcgacg acctgcttgc gcagcagcag catcagcagc atcagcgaca gcgccgcgaa
    14581 gaagtacacg aagccgaagc cgaccgggat gccggcgatg tgcacgctgt cggcgcggct
    14641 cgtgagcgcc tcgccgagcg cggcggcggc ctgcgcgagc ggtgcgctgc cgttgaccag
    14701 ccgctgcgtg gcgtcctgcg cctggaacag ctcgggcgcg taggcgagca tctggccgat
    14761 cgatttttcg agctgcgcgg caccggcggt gatgtcgttg agcttcggca gcagcgccgg
    14821 ggcgttcatc ggcgtcaggc cctggcgcgg atcgccttcg cgcagcgcct tcgccagccg
    14881 gccgaactgg ctgacatcgc gcagcagccg gtcggcggcg agcgccgagt tggcatcgcc
    14941 gcgcaccagc agcccgaggt cgtactgcat gcgctgcgcg agcgtcgaca gccgggccgc
    15001 ggcgcgcacc tgcgcgcccg gcgcgccgcc atcggcgagg ccgccggaga ggtcatcggc
    15061 gtagtcgtgc agggccggca gggtttcctc gaataccggc agcagcgcgc gcacggcctt
    15121 gaccggctcg cgcgattcga gcacggcgct gacggcgccg cgcgtctcct gccagcgctt
    15181 ttcgagctgc gcgagcgcgt caccggcttc ggtcggcacc ggcgccgtgg tggcgtcgcc
    15241 gttgcgcagc gccgcgatcg agctgtcgaa gcgcgtgcgt gcctggccga ggcggtcgaa
    15301 ggcgtcgtcc tgaccctgca ccgcctcgac cgccagcgcc gtcagcgtgc gggccagcag
    15361 gcgctgttcg ttgaccaggg ccaggtcggc ggtgatgccg gtctcgtgcc gctgcaccag
    15421 aaagaatgtg cccgccatca gcagcaccga cacgaccagt gccacgccgt acagcgcgaa
    15481 gcggggtgcg ccgccggggc gcgcggtggt gttgcggggt tcgctcattg tgcttgtgct
    15541 ccctggtgta agcgcccggc gtgtggcgcc gggcgcggcg ctgccggctt ccgggctgcc
    15601 gctggggggt taaagggccg ccgccatgaa tcgcggatcc gcgagcaggc gaccgatgtc
    15661 gaattcactc cagacctggt tgtcgatcgc gtaggccgcc cgggcatagg gcttgagcgt
    15721 cggggcggtc ccggccggca gttccggcag gcggttggcc ggaccgatcg ggcgcatgcc
    15781 gatgatctcg tcgaccacga ggccgtagga ccagtcgccg ccgctggcga tgacgatgcg
    15841 cgtgcgcgga ccgatgctgg tgtgccgtcc ttcgaggaag tcgccgaggt cgatgaccgg
    15901 cacgatgcgt ccgcgcaggc tgacgatgcc gagcagccag ggctgcacgc ccggcacgcg
    15961 cgtcaggcgc ggcaccggca cgatctcccc ggtttcggcc agcgcgaaca gcagcgcgtg
    16021 ggcgccgacg cgcaggccga gcgcaccgct gctggtcggg gacgcggcct gggccgcgct
    16081 gccctgtccg ctgcggcccg ccagttcgag gagctgatcg aacggatgct gggcggcggc
    16141 catggccatg tcggctcagg tccgcttgcc gagcagggtg tcgatcttgg cgatcagctc
    16201 cgcctcgacc ggcggcttga ccacgtattc ctgcgccccc tggcgcaggc cccagatgcg
    16261 gtcggtctcc tgatccttgg tcgtcaccat gatgatcggg atgtgggcgg tcgagggggt
    16321 cttggcgagc ttgcgggtgg cctggaagcc gttcatgccc ggcatcacga cgtccatcag
    16381 gatcaggtcg ggatgatcgg cctgggcccg ggcgatgcct tcctcggcgt tgccggcact
    16441 ggcgacctgg tggccgcgcg actcgagaat ccgcgtcagc gcgaagatct cggtcggaga
    16501 gtcgtcgatg atcaggatgc gggccatcgt gtgatttccg tgtgcgtacc cgtgaggggc
    16561 gggccggccg tcgttcgacg tcttccctga gttcacttcg acacgtggac gtgctgctgg
    16621 atggcctcca gcaggtcttc gcgtgtgaac ggcttggtga tgaactgttc ggaaccggcg
    16681 agcttgccgc gcgccttgtc gaagatgccg tccttgctcg acagcatgat gaccggcgtg
    16741 gcgcggaagc ggccgttgct cttgatcacg gagcaggtct gatagccgtc gagacgcggc
    16801 atcatgatgt ccaggaacac gatgtccggt tcggactcga ccatgcgcga cagtgcctcg
    16861 aaaccgtcgt cggcggtgat gaccgtgaag ccctcgcgtt cgagcagggt ctcggccgtg
    16921 cgccggatgg tccggctgtc atcgaccacc atcaccttga tcgcggagtt cgcgccgcct
    16981 gcttccactg aagaatctcc cccgttgcgc gacgcgcgca tcgggtgcgc gtctgaccgg
    17041 ccgggccggt ctcccctcgg accggaccag cgtcactccg gttatcatgg tcggccttgc
    17101 cgcacgctgc cagcttcgag cctgtctcca ccacggtcgc agaccataac cgaattttct
    17161 caggactgga aagcccgcgc ccccggcgcg cggcggccgg tccgcagccc cccgggagac
    17221 ccgatgaaca agcgcctcgg cgtggtcatg gaccccatcg ccacgatcac catcaagaag
    17281 gacacgacct tcgcgatcct gctcgccgcg caggctcgcg gctggtcgat ccggtacttc
    17341 gagcagtccg acctctacct gcgcgacggc cgcgcctgcg gccgcgcgcg cgcactgacg
    17401 gtgcgcgacg acaggcagga ctggtacacg ctcggcgaac cgcaggatga gccgctcgcc
    17461 ggcctcgacg tgctgctgat gcgcaaggac ccgccgttcg acctcgaata catctatacg
    17521 acctacctgc tcgaacgcgc cgaggccgag ggcgtgcgcg tcgtcaaccg gccgcagtcg
    17581 ctgcgcgatg ccaacgagaa actgttcacg gcctggttcc cgcagtgctg cccgccgacg
    17641 ctggcgacgc gcgacatggc gcgcctgcgc ggcttcgtcg ccgagcacgg cgatgcgatc
    17701 ctcaagccgc tcgacggcat gggcggggcc ggcatcttcc gcgtgcgcgc cggggacgcc
    17761 aacctcaacg tcatcgtcga gacgctgacc gagcgcggcc ggcgcaccgc actggcgcag
    17821 cgcttcatcc ccgagatcag cgccggcgac aagcgcatcc tgatgatcga cggcgagccg
    17881 gtgccgtatg cgctggcgcg catcccggcc gaaggcgaga cgcgcggcaa cctcgccgcc
    17941 ggcgggcgcg gcgtcggcca gccgctcagc gagcgcgacc gctggatctg cgcgcaggtc
    18001 ggccccgagc tgcgccgccg cggtctggtg ttcgtcggcc tcgacgtgat cggcgactgg
    18061 ctcaccgaga tcaacgtcac cagcccgacc tgcgcgcgcg agctggacgc gcagttcggc
    18121 ctcgacatcg gtgccgacct gatgcgcgcg ctggaggcga cggcggcatg aggctgttca
    18181 gcttcgccgg ccgccggcgg gtggccgcgc tgctcggctg cgtgttcgca ctgctgctcg
    18241 ccgcctgcga aaagcccgac ccgctgcagg cgacgcgcct gctggtgttc ggcaccgtcg
    18301 tcgatgtcgc cacctggggc gtgacgccga agccggcgca ggcggccttc gacgagctgc
    18361 gcacggcctt cgagtcctgg caccgcgagt ggcacgcatg ggaggactcg ccgctgtcgc
    18421 gcgccaaccg ggccttcgcg gccggggccg gggctgcgct gactcccgac ctgctgcggc
    18481 tcgtgagcga tgcgcgtgcc tggtcgcagg cgagcaacgg cctgttcgat ccggcgatcg
    18541 gccggctcgt ggcgctgtgg ggcttcaatc agggcgagcg gcccgacgcg ccgccgccgc
    18601 cgccgcaggc gatcgccgaa ctcgtcaacg cgcatccgtc gatggacgac ctgacggtcg
    18661 acggcgagca gctgcgcagc cgcaatccgc agctctggct cgacttcggc ggcatcgcca
    18721 agggctacgc ggtcgatcgc gcgatcgagc ggctgcgcgc gcgcggcgtg cagaacgcca
    18781 tcgtcaacgc cggcggcaac ctgcgtgcga tcggcagcaa ggacggccag ccctggcgca
    18841 tcggcgtgcg cgatccgcgc ggcccgggcg tgctcgcctc gctgaccatc accggcgacg
    18901 aatcggtgtc gacctccggc gactacgagc ggcacttcga atggcagggc gtgcgctatc
    18961 accacgtgct cgatccgcgc agcggctatc cggccatcgg cgcggtggcg gtgaccgtcg
    19021 tcgccgagag cgccgagaag gccgatgtcg cctcgacggt gctgatgatc gccgggccga
    19081 ccgaatggcg ggcgctggcg cgcgcgctcg gcgtcacgca ggtgctgcgc atcgacgagt
    19141 ccggccaggt cgagctgacg ccggcgctgg cggcgcggct gcactgggag gtgtcgccac
    19201 cgccgacgct cacggtcgtg cccgagccat gagccctccg gcccccgccc tgcacccggc
    19261 gcttcgcggc cggctgaccg ccggcgacgc cgtggtgctc gcgctgtcgc tcgccgggct
    19321 ggtcgcgctc gccgtgcagc tctggggcgc cgatgcgcgc ccggacacgg cgctggtgcg
    19381 catcgacggg cgcgaggtgc tgcgcctgtc gctcgccgag gccggtcagt acgcggtcga
    19441 gggaccggcc ggcacgacgc gggtcgaggt gcgcgacggg cgcatccgct gcgtgtcctc
    19501 gccggggccg cagcatctct gcgagcgcgc cggctggctc tcgcacgccg gcgaggtcgc
    19561 cgtcggcctg ccgaatcgcg tcagcatcga ggtgctcggc cgcaaccggc gcttcgacag
    19621 catcagctac taggcgcgga cggccgccgc ttcacgatgc ctgccgcccg tccgccgctg
    19681 gagccgctca cgctcgccgt cgatgccgca gaccggcgca tcgcctggta cgcggcgctg
    19741 gcgctggcgc tgcacgtgct cgaagcggcg ctgccgagcc cgttgcccgg tctcaagccg
    19801 gggcttgcca atgtgctggt gctgatcgtg ctggtgcgcc acggcctcgc cgccgccgcc
    19861 tgggtcgccg gcctgcgcgt gctggtcggc agcctgctgg tcggcacctt cctgtcgccg
    19921 gccttcctgc tgtcggcggg cggcgcgctg gcggcgctgc tcgtgctcgc gctcgcgcac
    19981 gccagccgct gcttcgggcc ggtcggcctg tcgctgctcg cggccgaggc gcacatggcg
    20041 atgcagttcg cgctcgcacg cctgctgctg gtgccgcacg cggcgctctg gcagctgctg
    20101 ccgctgctga tgacggcggc ggtcgcgacc gggctggccg gcggtatcat cgcccgcgcg
    20161 ctgctgcgca ccgatgccgc cgccggaagc cgcccatgaa cgccatcctt tatgcgcacc
    20221 tgctgcagga gcgcccgtcg cgccgcctgc cgctggcgct gctgttcgcg ctcggcgtgc
    20281 acgtggcgct gctcgccggc atcggcctcg gccggccggc cgtgcccttc gatccggtgc
    20341 tgtcgttcaa ggtgtcgctg gtcgagcgcg ccggccacgc cgagcgcgat gcgccgctgc
    20401 gcgcacccga gccgcagccg ctgccgccgg tgccggccga gccgccgccg gcggtgatgg
    20461 ccgagcccga acccgagccc gagccgcagg cgtcgccggt ccctgcgccg cccgtgatca
    20521 gcacgcaggc accgccggcc ccggcgcggc cggtggtcgc cgcgccgcga caggtcgaac
    20581 cgaaaccggt ggagccgaag ccggtcgagc cgagaccggt ggaaccgaaa cccgtgccgg
    20641 ccccgcgccc ggtggcgccg ccggcgccgc ggcgcagcgg accgctgagc gccgccgagc
    20701 tgatgggcgc cggtctcgac tcgatccgtc aggaggcccc gctgccggcg gcgagcagcc
    20761 gcgggcgcag caagcgcgcc gatccgaccg acagctcgac gctggaaggc tattacgccg
    20821 cagcctgggt cgcgaaggtc gagcgcatcg gcgcgctgaa cttccccgag gaagcccgcc
    20881 gacgcaacct gaccggttcg ctgcgcctgt cggtggtgat ccgcgccgat ggcacgatcc
    20941 tcgaaaccac cgtcgtgcgc tcctcgggcc agtcggcgct cgacgagggc gcgcggcgca
    21001 tcgtcgagct cggcgcgccg tacgcgccgt ttcccgatga gctgcgccgg cgctacgaca
    21061 cgctggtgat cgtgcgcgac tggcagttcc gccagggtgc gcagttccag tgacgggcgc
    21121 ttgcgcccgc gtgccgcacc gtcgtgtctt gtggaagtcc cggacgctca cgatactgcc
    21181 gccatgctcg aaccctcgta tctcagccat cagttcctga tcgcgatgcc ggcgctcgcg
    21241 gatccgaact tcgcgcgcac cgtgacctac ctctgcgagc acggaccgca gggggcgctc
    21301 gggctgatcg tgaaccggcc gctcgatctc accaccgccg acctgctcga aaacctcggc
    21361 atcgaggcgc cgcccggcac cggggcgctg ccggtctact tcggcgggcc ggtccatccg
    21421 gagcagggct tcgtgctgca ccggccgctc gggcgctggc agtcgacgct cgcgaccggc
    21481 gacggactcg cgatcaccac ctcgcgcgac atcctcgaag ccatggcccg tggtcaggga
    21541 ccggccgagg cgctgatcgc gctcggctac gccggctggg ggccgggcca gctcgaacgc
    21601 gaacttgccg agaacgcctg gctgtcctgc ccggccgatg ccgacgtgat cttccggctg
    21661 ccggcggccg agcgctgggc ggcggccgcg gcgctgctcg gcgtcgatct gcgcctgctc
    21721 tgcggcgacg ccggtcacgc ctgacgtgcc cgcgagcgcg ccccggccgc ggaccgtgct
    21781 ggccttcgac tacggcagcg tgcgcatcgg cgtcgcggtc gggtcggagc tgaccggcag
    21841 cgcccggccg ctcgcgacgc tcgccggccg cgactggacg gccatcggcc ggctgatcga
    21901 cgaatggcgg cccgatgcct gcgtcgtcgg cgtgccgcgc cacgccgacg gcagcgaatc
    21961 cgaaaccacg cgcggggcgc tgcgcttcgc gcgccagctc gacggccgct acggcctgcc
    22021 ggtcgcgacc cacgacgagc gcctgtcctc gtacgccgcc gccggacgtc tggcagccgc
    22081 cccggcctcg cgccgccggc ccggtccggg gctcgatgcc gccgccgccg ccctgatcct
    22141 cgaaagctgg tttgccaccg tcaggaacga tccgccatgc aactcgacgc cgaacgcctg
    22201 atcgacaccc tggccagcgg gctgcgcgcg catctggccc ggcgcggcat cgggacgccg
    22261 ctgctgttcg gcatccgcac cggcggggtc ggactcgccg agcgcctgcg cgtcgcgctc
    22321 ggcgtcgccg agccgatcgg cacgctcgac atctcgtttc accgcgacga cgacgtgcgc
    22381 gacggcctcg acccgcaggt gcggccctcg cgcatcccgg tcgatcccga aggccgcgat
    22441 ctggtgctgg tcgatgacgt gctctacacc ggtcgcaccg tgcgcgcggc gctcaatgaa
    22501 ctgttcgact acggccggcc gcgctcggtg acgctggcag tgctggtcga tcgcggcgga
    22561 cgcgagctgc cgatccaggc cgacgtggtt gccgagctcg tcacggcggc acccggcgag
    22621 cgcgtcgagc tgcgctggcc gggcggctga gcggccggct gcggcggcgt gcatcgtcgc
    22681 gatggtatgg cacggctttc cgcagtaatt ttgttgtttc ccgctgtatc ccgtatcgaa
    22741 gcttccgagc cgtccgctgt cgcccgacga cggcgccggc gcacgcaccg ggtcgaccag
    22801 gcatcgccat gactccatcg tccatccagc tcgacgccga cgggcgtctg cgccacttcc
    22861 tgaccgtcga gggcctgaag cgccggcatc tggtcgagat cctcgaccgc gccgaggcgc
    22921 tctcgggcgt cgccgagcgg cgcatgacca agtccccgac gctgcacggc aagaccgtcg
    22981 tcaacctgtt cttcgagaac tcgacgcgca cgcgcacgac cttcgaactc gccgccaagc
    23041 gcctgtcggc cgacgtgctg aacgtcaaca tcagcacctc gtcgacgcac aagggcgaga
    23101 cgctgctcga caccatccgc aacatcgagg cgatgcaggt cgacatgttc gtcgtgcgcc
    23161 acgcggtttc gggcgcggcc gagttcatcg cccgccacgt cgcgccgcac atcgcggtgc
    23221 tcaacgccgg cgacggccgc catgcgcatc cgacgcaggc gatgctcgac atgatgacca
    23281 tccgccgcca caagggtgaa ttcgcgaacc tgcgcgtcgc catcgtcggc gacgtgctgc
    23341 actcgcgcgt ggcgcgctcc gacatccatg cgctgaacat cctcggcacc ggcgaggtgc
    23401 gcgtggtcgc gccgaagacg ctgctgccgg ccgatgtcgg cacgctcggc gtgcacgtgt
    23461 tccacgacct cgaacacggg ctgcgcgggg tcgatgtggt catcatgctg cgcctgcagc
    23521 gcgagcgcat gagcggcgcg ctgctgccct cggcttccga atacttccag cagtacggcc
    23581 tgaccgccga acggctcgcg ctcgcacagc ccgatgccat cgtcatgcac ccgggtccga
    23641 tcaaccgcgg cgtcgagatg gactcggccg tcgtcgacgg cccgcgctcg gtgatcctcg
    23701 aacaggtcac ccacggcatc gccgtgcgca tggcggtcat ggcgatcgtg ctcggcaacc
    23761 gccatccgga catcaccgag gagacctgag gccatgcgca tcacgctgca aggcggtcgc
    23821 gtgatcgatc ccgcccacgg cctcgacgcc gtcaccgatc tgcacatcgc cgacgggcgc
    23881 atcgccggtc tcggcgccgc gccggccggc ttcatcgccg agcgcagctg cgacgtgtcc
    23941 gggcgcatcg tctgccccgg cctcgtcgat ctctgcgcgc ggctgcgcga gccgggcgcc
    24001 gagcacaagg cgaccattgc ttccgaaacc cacgccgccg ctgccagcgg catcacgacg
    24061 ctggtgatgc cgcccgacac cgatccggtc atcgacgaga ccgccgtggt cgaatcgatc
    24121 cgccgtcgcg cgcaggccgc gggcttcgcg cgcgtgctgg tgctcggcgc gctgacccgc
    24181 ggcctcggcg gcgaggtgct ggccgagatg gcggcgctga aggaggccgg ctgcgtcggc
    24241 gtcggcaacg ccggcgtgcc ggtgccgaac tcgatggtca tgaagcgcgc gatggaatac
    24301 gcctcttcgc acggcctgac cgtgttcctg acgccgatgg acccgtggct gtcggccggt
    24361 ggccaggccc atgacggcca ggtcgccgcc cgcctcggtc tggccggcat cccggtcgcg
    24421 gccgagaccg ccgcggtcgg gcgcgagctg gcgctggtcg aggaaaccgg cgcgcgagcg
    24481 cacttcgggc ggctgtcgag tgcgcgcgcc gtcgcgatga tcgcgcgtgc gcgcgatcgc
    24541 ggcctgccgg tgagctgcga cgttgccgcg caccagctgc acctgaccga ggtcgacctc
    24601 ggccgcttcg acgccgatgc ccacgtgctg ccgccgctgc gcaccgcgcg tgatcgcgat
    24661 gcgctgcgcc gcggcgtcgc tgccggcgtc atcgaggccg tgtgttccga tcaccagccg
    24721 cacgaacccg acgccaagct cgtgcccttc ggcgacaccg agccggggct gtcggcgctc
    24781 gacaccctgc tcggcctgat gctgaagctc gtcgatgaag gggtgctcga cctgccgggc
    24841 gcgatcgagc gcgtcaccgc cgggccggcg cgcctgctcg gcatcgaggc cggcacgctc
    24901 gccgtcggca cgcccgccga tgtctgcgtg ttcgcacccg gggcccaccg cctgctcgat
    24961 gcgcgcacgc tcgcgagccg cggccacaac accccgttcc tcggctggga actgcgcggc
    25021 gtcgtgacgc tgacgctgct cgacggccgg ccggtctacc agcgcgaggt ctgacgacca
    25081 tggacacttc ccgcagcgtc cagcgttccg gatgtaccga actgcccggt ggctggcgcg
    25141 tcgagcgctg gcaggccccg gcgctggtcg atcatgcccg tcccggccag cggctggccg
    25201 ccggcagcgc cgagctgccg gtcatgcgca cgcgcgcggc gaccggcgaa ctcgaatgcc
    25261 tcgcacgagg cccggccggc gccgcggtcg ccgcggccga tgccgacctg cgcggcgagc
    25321 ccttcgagct gtcggcgccg agcccgcgcg cgctgctgat cggcgatctg tccgggctcg
    25381 cgccgctggt gttcctcgcc gaccggctgc gcagcgccgc ggtgcgcgtc aagaccttcg
    25441 cgatcctcgg gctcgacggc gaggcaccgt tccgcccggt gccctcgcgc ctgatcgtgc
    25501 ccggcgtgcc ggcctgggtg accggcacgc tgccgctgtt cgaggactgg ggcatcgccg
    25561 cgcgcctcgc cagcgccggc gaggaccggc ccggctgctt cgagggcacg ccggtgcagc
    25621 tcgcccgcgg ctggctcgca gcgcagcagg gcgtgcgcga tgtctgcgtg tatgcctgcg
    25681 ccggcccggc gctgctcgaa gacacccgcg cgctggcagc cgcgttcggt ctcggttatc
    25741 agggacgcgc tgccggcagc gcgtgctgac cggcatccga tgagcgcccc ctccggcgac
    25801 gacggggcag ggcggcgcca cccggcgcaa ctcgccgcgc tgctgcagcg ccaggcaccg
    25861 acgctgctgc tggcggtgct gaccttcgtc gcgatcggcc tgctgtcggg ggcgacgctc
    25921 tggcagatcg acaatgaccg ggtgcgcacg ctcgaggacg cgcgccgcgc caacgtccag
    25981 ctcgccgatg cgctcgccga gcacggttcg cggctgatcg agtccgtcga cgtgctgctg
    26041 cgcgatttct ccgaatcccc gccagcgccc ggcaccggcg cgcttggcgg cgatttccgc
    26101 gaggcctacg cccagagcct gctgttcctg cgcaacctcg ccctgctcga cgccgacggc
    26161 cgcatgtacg cgacgctgta ctacagcgag atgcgcgggc gcagcttcgc cgggcgcgac
    26221 tactacctgc tgcaccgcgc ccaggccgat gccgggctgg tggtgtcgac gccgaaggtg
    26281 tcgccggccg acggccgctc gcgcatcctg acgctgtcgc gccggctcaa cgatgccggc
    26341 ggggccttcg ccggcgtcgt ggtcgcctcg gtcgggctcg acacgctgga gcgctattac
    26401 cggtcgatcg agcgcgcgcc cggccagggc atcgaactgc gcctgcgcga cggcgaggcg
    26461 atgctgagcg tcggcgtcga gcacaatgcc ggccccgacg cgctggaagc cttgcgctgg
    26521 ctcgccggca cgccgtacgg gatcgtggtc agccggctgc cggtgcgggt gctcgccgac
    26581 tggcgcgagc gcaccggcag catgctggtc gccgtggccg ccgccgacac gctgatcgcg
    26641 atgctggcgc tgctgctcgg cgggcgcctg cacatcggcg cccgcctgct cgggcggctc
    26701 gacgacgccg agcgctggca gcgcacgctg ctgcgcacgc tgcccgaccc gctggtcgtg
    26761 ctcgatcggc gcctgcgcgt gcgcgaggtc tgcagcgccc agccgcagcc cttcggcgcc
    26821 gacatcgagc tgcgccccgg cagcgcgctg ttcgaggtgc tgccgcctgt cgtcgccacc
    26881 cgcgtcggac cggccgccgc cgaagccctc ggtgatgacc gcatccgctc ggtcgactac
    26941 ccgctgcaca tcgacggcga actgcatcat tacgcggcgc gcatcctgcg gctggcggcc
    27001 gaggacggca ccgtcgaggg cgtgctgtgg ctcgcgcggg acatcaccga ccgcaagcgc
    27061 atcgagcagg cgctgttcca ggaaaaggaa cgcgcgcagg tgacgctggc ggcgatcggc
    27121 gacggcgtgc tggtcaccga tgaggaagcc cgcctcgaat acctgaatcc ggtcgccgaa
    27181 cggctgtgcg actgccggct ggccgaggtg cgcggctggc cgctcgccgc ggtgctgcgc
    27241 atcgaggccg aagccggcag cgaactcggc agcgatccgg ccacgcagtg tctggccgac
    27301 aaccgcccgg tcggccccgc cctgcatgcc cggctcatgg ccgcggacgg gcgcgagcgc
    27361 cacgtcgaga tcagctgcgc accgatccac gacccggccg gccgcccgac cggcaccgtg
    27421 acggtcatgc acgacgtgag cgaacgccac cgcctgaccc agcagatccg ccaccaggcc
    27481 gcccacgatc cgctgaccgg cctgatcaac cgccgcgagt tcgagctgcg cctcgacgcc
    27541 gcctgccgca tcgccgatga ccagaaccgc acgcacgcgc tgctgtgcat cgacctcgac
    27601 cgcttcaaga tcgtcaacga cagctgcggg catctggccg gcgacgaact gctgcgccag
    27661 gtcgcgcggc tgatgaccga gcacacccgc cagcgcgacg tgctcgcgcg catgggcggc
    27721 gacgaattcg cactgctgct cgaagactgt ccgctgccga aggccgccga gattgccggg
    27781 gcgctggtca gcgcggtcgg cgagttccgc ttcgtgcatg gcacgcaggc gttctcgatc
    27841 ggcgccagca tcggtgtcgc cccggtgccg cccggcaacg gcgggccgca ggtggtgctg
    27901 agccgcgccg accgcgcctg ctacgcggcc aaggacgccg ggcgcaactg cgtgtcgctg
    27961 gccaatgagg acggcgatgc cgcgagcagc ggcacccgcc actggcacga gcgcctcgaa
    28021 gcgctcctcg acggcggctc acaggactac gaactgcacg tgcagccggc ctcggcgctg
    28081 tacgccggcc tgccgccgta ccgggaggtg ctgctgcgcc tgcgcgacga cgacggccgg
    28141 ctggtgccgc cgggcgcctt cctgccggtc gccgaccgct accggctcgc agccggcctc
    28201 gaccgcttcg tgctcgatgc gctgtgccgc cggctcgcgg agggcgccgg cggcatcgac
    28261 ggcctgcacg ccatgaacct gagcgtcgcc acgctggagg acggcggctt tgccgggcac
    28321 gtgcgcacgg cgctgcagcg ccacggcctg gcgccggccc ggctcggctt cgagatcgag
    28381 gaagccgccg tgcacggccg gcttgcgcag gccggcgagc agatcggcgc gctgcgcgcc
    28441 ctcggctgcc gggtcaccct cgatcgctgc ggcagcggcc tcgcggcctt cgactgcctg
    28501 aaggtgctgc gggtcgatgc gctgaagatc gacggcagcc gcctggaggc cgaaggcagt
    28561 cacccggtcg acaccgtctc cgccgaagcc atcgtccgcc tggcccgcct gctcggcctg
    28621 cacacgatcg ccagccgcat cgaaaccacc ggccagcaca gcggcgcggt cgtgctcggc
    28681 atcgatta
//
LOCUS       DAOQIE010000011        27108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_770_length_27108_cov_7.207482, whole genome shotgun sequence.
ACCESSION   DAOQIE010000011 DAOQIE010000000
VERSION     DAOQIE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_770_length_27108_cov_7.207482"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1944)
                     /locus_tag="PK144_01415"
     CDS             complement(211..1944)
                     /locus_tag="PK144_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64410.1"
                     /translation="MSDHEQDWKRALAAISKDLRRQTAPPPGTSRRIRSSLPAQRTSA
                     RPDATAAAAGPAAAAKSPSPPAPALTPASPARSASSRPAPASPAPARPESPPSGKPPL
                     RGYGRGLSVYATTRKATAPASVPRTAEPITDPLAPSSLAIVPRRLPRQPDTALWRTGG
                     RLLQLDAGPRAARQRMTIGLDFGTAFTKVVVADGANRRYVVNFEAAVRARGAAACFLP
                     GFLAIDSGGLGWLGCDRNNRLLADLKFRLLEETLDAQTRAAIVCYLALVLRYTRHWLL
                     TQHRSVYANRRLEWFVNVGVPAARWRQAGIDDEYRQLVEAAWKLSVAEDEPTLAAAQS
                     VQQERPCLLGERLATVPEFIAQIAMYVNSPQRRPDLHLLVDVGAGTLDVSTFNVHESD
                     GEHVFPIFASRVERLGCHYAIRRALAGTSARVIEDAGFDRQKLAGIKGVTEAELQQRL
                     RTVENDVCRVISAVLTQTKGRRYRNSRAWSDGLPVLLCGGGREVALYRSAIERLIADR
                     YPLRLLHLNRPSNLVAPELPETALQRLSVAYGLSFAFENLGLIRTADTVEDDLPSADA
                     AHTYRDRYIEK"
     gene            complement(2026..5127)
                     /locus_tag="PK144_01420"
     CDS             complement(2026..5127)
                     /locus_tag="PK144_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNF64411.1"
                     /translation="MSRWQAGMPVRLLDDPGQTGVVTGLTRERGEVQWVQVRFPAGCQ
                     YYADYELETLDAAPADPYDRVLAGRFGRTEDLRRQLTRIQLSGRLANLIYSLDTTATD
                     FYAHQFKPVLSFLESPCNGLLIADEVGLGKTIEAGLIWTELRARLDARRLLVVCPAML
                     REKWRDELRRRFGIRAELVGAGELLAALREPKDTWPAERALVGSLQGLRPSGDWQSTD
                     EQGERAQLARLLDEQADAEPLFDLVVIDEAHYLRNPQSLSAELGRLLREVSAHLVLLS
                     ATPVNTRADDLFELLRLVDPENFRYGEQFQQVLLANRPLLRARACALDLRASLAQVVD
                     ELRKAQAHPLLAGSRQLQDLIAACTDGPAPDTRATRIELANRIERVNLLAHAVTRTRK
                     AEVQERRVVRRVRVEAVEPTDAERALYESVTDAVRGYAWQRDVSDGFLLAMPQRQVSS
                     SMAAACTSWLGREDALDAESLFECLTLDIDRPQGSPLVRRIAEELAGTVDTAELARHD
                     SKYQRLHEVLIEYLREHPDEKVVVFSYFRPTLHYLARRLEHDGLSACVLMGGMRESKQ
                     DVIDRFRDTPQTRVLLSSEVASEGVDLQFCRVLVNYDLPWNPMRIEQRIGRIDRLGQR
                     AESIFVWNLCYAGTIDERIVRRLYERLNLFETALGSLEDVLGDEIARLTQDLLCQKLT
                     SAEEELRIDQTAQAIEQVRQQQDELERQASTMIAHGGYILEQVNAAHTFRKTITAGDL
                     AAYVRDYLERHAPGHTLYQPGADPFEFDLRLPPAVAVRYANYLQVRHLLGQSRLGTGD
                     EVRCRFENRVGASSHSRIETISQFHPLPRFIGEELERLETSVWPVVALRLDRQRSGLP
                     SLPAPGIYAFALLLTSFSGMRTEEELLALAWPVDADAPLATDLSMDLVNAAKHHGADW
                     PGVAADVDLAGLAERTQDCFERLLQTYAGKRRAMEAANHDRIRFQLDALENHRRRKLD
                     QIEERLAKLRAAGRTRRVINATEGQRRKLEERIAIQRERLNERNGLKSDRLEVCRGLI
                     RIE"
     gene            5551..7335
                     /locus_tag="PK144_01425"
     CDS             5551..7335
                     /locus_tag="PK144_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF64412.1"
                     /translation="MPSLRRPPSAFHRARRAAPATALALGIALSLTFPVAADEVSRPP
                     AAPDPLYDPKPLADDIELPLPCDGRLLLRTVYVLAKSALDDREVNLGYPFTQDEPGYK
                     QGFVAGYRLGHVNGQFEPADLPTAWRTRLDGKLPAAGEGGLKPMLYFIGKYEITQRQY
                     RLVMALEPWLHGAAPEPPACPAPAATDRLPMVDLSWFDALNFTALYTEWLMRKHREAL
                     PALGGGAGSAAGGASAYVRLPTETEWEYAARGAQVVDKQALEARLFPRREGNASEDGP
                     LRDWAVYTQVAGGSGQTARLAPIGTKRPNPVGLFDVIGNAAEMVLDPFLLVRGGGRPG
                     GGTGGFVVKGGNYLEGEQTLFTGMRREYPLFDDKGQPSRNTTTGFRIALGTLAAPRAR
                     YDELFAGWQAEGRLSNLTSELPPDADAGVKLDRIVADLQRDMPTSTIGRELGTVSEEL
                     KRSVALIAAQRRDAAANLIQSSALVAETIGNYNIRLTNLNKQLEEQKAKRNAEGVQFF
                     TNVIADGTSAVQAALSIYLDNVNSGAAYAETLFAEQFQRVQDELGKKPVLGRSLSARA
                     TLFMKHVEQYRETRRLDRDRVLNEVLGR"
     gene            7350..7877
                     /locus_tag="PK144_01430"
     CDS             7350..7877
                     /locus_tag="PK144_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64413.1"
                     /translation="MRFLLAPLAVATLAGCCVCAPSPAPVAAGDVPVAPGTPPLTDFY
                     ADCGRGLLDAARLELELERDAGRAAVWNGYIAATQRTQDCYWAAYQPLLAQHRSGSLN
                     RVEGRARLRELGDGIAATRVALARNLRRPEAVARAADMQRQIAQADNRLREFCTNPDV
                     GDWLPPGFDCTRLSV"
     gene            7971..8873
                     /gene="tagQ"
                     /locus_tag="PK144_01435"
     CDS             7971..8873
                     /gene="tagQ"
                     /locus_tag="PK144_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated lipoprotein
                     TagQ"
                     /protein_id="HNF64414.1"
                     /translation="MHALPPQTGARRLAAALSLTLALGGCAGVQLGGPADTVAQTTSV
                     NYYPACYEPVRYLRESDDKMKSAVATGAVGGALAGGLLGALTGGDNAGRNALIGAAAG
                     ALIGGASSYYLEKQKQNAEDSARFAAYGTDIDRATGEYNRTTSAARNAQGCYQKEFQT
                     LVQLRKTRRISDTEGRARFNEIVSGISEANALMAAVDQRIGESLNTYTQAYEEDLKKT
                     GVERQAVTRAARSSKPVAVAGSAKPASTEAAVNTERKVQQASTARDESKAVSSGLTAQ
                     LKSFCSNPDVGDWGSGASACGRGV"
     gene            8947..9828
                     /locus_tag="PK144_01440"
     CDS             8947..9828
                     /locus_tag="PK144_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNF64415.1"
                     /translation="MSLSEPKPGQRPLAAALSLLLALSGCAQMNSTMGVVQQTTVNYY
                     PKCYEPVGYLRESDQRMQQAVGTGVALGALGGAAIGAIATGDSRGAIAGAVLGGLAGG
                     ATAYYQQKQQQLAEDSARFAAYGTDIDRTTGEFNRTTSAARNAQRCYQKEFKALVQLR
                     KRGKVSDAEGRARFAEIVSGMSEANALMAAVDGRIGESLDTYTKAYESDLQKRGATRS
                     TVAQSANRGGGRGSSKSSGVPTEAVNVERKVQKATAAREEVKTVTASIAADTRSFCSS
                     PDIGDWGKGNATCAKSA"
     gene            complement(10069..13647)
                     /locus_tag="PK144_01445"
     CDS             complement(10069..13647)
                     /locus_tag="PK144_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64416.1"
                     /translation="MNDDAALLQALFRAIPDMVWLKDIDGRYLACNPAFERYYGQMAE
                     AILGHTDFDFVDADTAAFYRRKDAEAAAAGVPRRNEEWIVYPDGARRLIETTKTPLHT
                     GNGQLLGVLGIARDITERKQAEEAALDQQARLRALLDSFPFPVWMKDADCRFVAVNRA
                     VALLTGHADPAALIGHEDLDFFPPDLCARYRADDLAVLRDGLPRQVEEPIEARGERRW
                     FETLKFPAFGTDDRVIGTVGYARDVTVRRDAEERLRAYERDYRHFFEDGRVAMLLVDP
                     ADGSIVDANHAAAIFYGWSRPQLLGLNLSGLSMLPAGALASRIEEAVAAAQTHFLCRH
                     RRADGSEPDVEVHAAPVRARGRSLLYLIVHDVTERVALQSALADSEARYRLLFENMHE
                     LLFDIAPDATVRLLNPAWTTITGHPLSATLGTDFRTWLHPDDRAPASLLFFELAAGRR
                     ERATIELRVRTADGGWRWLQTEGNPTRDAAGHITGIGGVAVDVSERREAEQRLRLAAS
                     VFDHAHEGIIITDAAGTILEVNRAFTELTGWTRDEILGENPRVFRSGRQNAAFYDRMW
                     STLAERGWWRGEVWNRRKDGSLYAELLDISAVHDSAGCLTHYVGVFTDITLLKEHQQR
                     LEDLVHHDPLTGLPNRVLLADRLAHAMAQADRAGTRLAVCYLDLDGFKPVNDTWGHET
                     GDRLLIEIAQRLLGCVRAGDTVARLGGDEFVLLLGGLPERDDCTGALPRLQAAVCTPC
                     VLDGRVLAVSASIGVTVYPDDASDADTLLRHADQAMYSAKQAGRNGFHLFDADQERRA
                     RDRSELIGRVHAGIPAGEFELHYQPEVDMHNGGVAGVEALLRWRPAGQPLLSPAAFLP
                     AIEASPAEIALGEWVLDSAIAQLERWHGAGLALTVAVNVSAHHLQHPGFVARLAQRLR
                     AQPHVPGRLLRIEVLENAALGDVARVRQIIADCRALGVGCALDDFGTGYSSLTHLRRL
                     PADVVKIDQSFIRDMLDDSDDLAIVEGVIGLARAFDRRVVAEGVETAEHGAVLLAFGC
                     THAQGYGIARPMPADALPDWLARWQPPALWQHHAARWTRAELPLLGARIELQRWIERL
                     GTCLDLPPGEIGNTPAFGTHDCRFGRWYHGPGRQRYGTHPVWAKIDAGHEEFHALGAE
                     LLAMHLAGEAEAARARLGELRTQCDALLGLLDALLGSPEREHTDSP"
     gene            complement(13785..14183)
                     /gene="pcaC"
                     /locus_tag="PK144_01450"
     CDS             complement(13785..14183)
                     /gene="pcaC"
                     /locus_tag="PK144_01450"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HNF64417.1"
                     /translation="MSPEQFEKGLEVRRAVMGDAFVERALANLDDFSRPLQELVTGYA
                     WGEIWTRPGLDRRTRSLINLAMLTALNRGTEFKGHVRGAINNGCTREEIVEVLLQAAV
                     YCGMPAGVEAFRNAREVLDQIDAERAGGSQ"
     gene            14348..14548
                     /locus_tag="PK144_01455"
     CDS             14348..14548
                     /locus_tag="PK144_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNF64418.1"
                     /translation="MTEAEFADWSLRLLLPALIGWMGFIVWGLARESKAGKFGTFVMF
                     GVLGLGVLGFIAKNVITFMLGL"
     gene            complement(14571..15365)
                     /locus_tag="PK144_01460"
     CDS             complement(14571..15365)
                     /locus_tag="PK144_01460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF64419.1"
                     /translation="MHIDLSGRTALVTGSTAGIGHAAALGLAGCGAHVIVNGRHADAV
                     EQACAAIRAAVAGAEVSGISADVGSAEGCAQLLAALPAVDVLVNNVGIYGPQDFFELS
                     DETWQRFFEVNVLSAVRLARAYVPGMATRGWGRVVFLSSESGLNIPADMIHYGFTKTA
                     LLSVSRGLAKRMAGSGVTVNAVLPGPTLTEGVQAMLQAEVEKTGQPIEAVGAAFVAAH
                     RPSSILRRLASAEEVANLIVYACSPQASATTGAALRVDGGCVDTIA"
     gene            complement(15736..16212)
                     /locus_tag="PK144_01465"
     CDS             complement(15736..16212)
                     /locus_tag="PK144_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNF64420.1"
                     /translation="MAHIIWSNDLSVGIEIIDSQHKRIVDYLNELDDARLKHSRDVVG
                     GVIEDLIDYTQSHFGFEETLMEEAGYPYVNAHKKIHELFIRRINEYHDRFKLGDDVTE
                     ELHRTLTNWLVNHIKREDNDYSSVVQVHTARGKVDLEPETSGWLGGALKRFFGKRK"
     gene            complement(16367..18034)
                     /gene="ubiB"
                     /locus_tag="PK144_01470"
     CDS             complement(16367..18034)
                     /gene="ubiB"
                     /locus_tag="PK144_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNF64421.1"
                     /translation="MLRPAQALRLLHINRVLVRHGLDDIILATHLFRPIGFVRHLLPW
                     NWLRGEDHRSRGERIRLTLEDLGPIFVKFGQMLSTRRDLLPDDIADELAKLQDRVPPF
                     APEQSRQIIEQAYGRPLAETFAEFGEQPLASASIAQVHLARLFDGREVVVKVVRPGIA
                     RTIRRDIELLYIVAGLAQRYWREGRRLRPVEVVAEYEKTIFDELDLIREAANGAQLRR
                     NFEHSPILYVPEIHWPYCRREVLVMERVRGIPVGQIEELRRLGVDMKKLSERGVAIFF
                     TQVFRDSFFHADMHPGNIFVDASDPADPRYIAIDFGIIGTLSPVDQHYLAENFLAFFR
                     RDYRRVAELHIESGWVPRDTRVDEFESAIRTVCEPIFDRPLREISFGGFLLTLFQTAR
                     RFNMEVQPQLVLLQKTLLNIEGLGRQLDPELDLWKTAKPFLEDWMNERIGPKSLLNKL
                     KANLPKLAEHLPDLPVQIGELLHALKSGELRMQVHARSLDALREDIRRQGARSRQTTV
                     GAALLIGAAVVYGLNSLNPAVVAAAPFVTWALGLSGGALVLGAALRD"
     gene            complement(18094..18699)
                     /locus_tag="PK144_01475"
     CDS             complement(18094..18699)
                     /locus_tag="PK144_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNF64422.1"
                     /translation="MPPMLLIAAAIETALNRLLAMDPEAPARLAGLRGATIAFEVHGV
                     NLLVYLVPVPHGFQVVHSHHGVPEVLVRGSPAAFLLLARGAPVQEAGVEIVGDPLAGQ
                     ALQKLLRELDIDWEEQASKVFGDVLAHRLGRFARGALGWLTQARETLFYDGAQFIQQE
                     AGALPVADRVHELLDAIDTLRADADRLDARLARLEARLASR"
     gene            complement(18762..19535)
                     /gene="ubiE"
                     /locus_tag="PK144_01480"
     CDS             complement(18762..19535)
                     /gene="ubiE"
                     /locus_tag="PK144_01480"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNF64423.1"
                     /translation="MASPETPGNDDTATTHFGYQQVPVEDKARRVAGVFHSVAARYDL
                     MNDLMSFGVHRLWKRYAIALAGLHRGDCVLDLAGGTGDLTRRMVSVVGARGRVVLSDI
                     NASMLDEGRKRLLDEGITGNVEYVLADAEALPFPDAYFDVVTMAFGLRNVTHKERALA
                     SIHRVLKPGGRLVVLEFSKPVVPGLAPVYDLYSFRMLPLMGRLVANDAESYRYLAESI
                     RMHPDQQTLRDLMQAAGFESVEYYNLTGGIVAVHRGYKY"
     gene            complement(19685..20047)
                     /locus_tag="PK144_01485"
     CDS             complement(19685..20047)
                     /locus_tag="PK144_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNF64424.1"
                     /translation="MSIRPTDLCLHQQTRILEVRFEDGAHFEMSCEYLRVFSPSAEVR
                     GHGPGQETLQVGKEEVEIVDIVPVGNYAVKLVFSDGHDSGLYSWNVLYELGRDQAANW
                     QQYLARLAAAGHVRRAGP"
     gene            20139..20663
                     /locus_tag="PK144_01490"
     CDS             20139..20663
                     /locus_tag="PK144_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64425.1"
                     /translation="MSLQGDNLHLQHAGGAECRPRPVVVAAKDRCITRMQAESDRYVA
                     FLRRYVIDLTCHCRKSVRGIDSTSEQPSDDILKKQPLAVRMPRGTKVAFHPVERSWRR
                     SADPKRGSAGPVVAPGAILPDPDVQHPEVLVMTNYISLRAVWHALKERGYWRMCAAMS
                     TDHPVPVSPNRRCL"
     gene            complement(20953..22332)
                     /locus_tag="PK144_01495"
     CDS             complement(20953..22332)
                     /locus_tag="PK144_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF64426.1"
                     /translation="MEVEMADEDEEEFERNLRLAEQGDADAQFEVGWMYAHGTGVSRN
                     DGEAARWFRMAADQGDADAQRNLGWMYAHGLGVDRDDREAVYWYRQAAEQEHPDAQCD
                     LGWMYEHGRGVIHDDKEAVRWYRKAAEQGQAFAQCNLGRMCEEGQGVEQDDRQARHWY
                     RKAAEQGHPGAIMELERHEIMVGPLEDDPPAEADASAPDDLSDLIGIASVKAQVESLT
                     QFIRVQNSRRAVGLKTPALSLHLIFTGNPGTGKTTVARRIGSIYRRLGLLQKGHVIEC
                     QRQDLVGEYIGHTAPKVRRKCDAAMDGVLFIDEAYSLMQPNSDKDFGSEAIAALVAEM
                     ENRRDRLVVIVAGHTQEMKAFLDANPGLRSRFWRTLHFEDYTAGELLEIFERFAIDGD
                     YRLDAQARSVLLGRFEKSLAGRTRTFGNARHARNLFEQALEKHAARVGRLADPDCAAL
                     QTITAFDLP"
     gene            22466..23569
                     /gene="dinB"
                     /locus_tag="PK144_01500"
     CDS             22466..23569
                     /gene="dinB"
                     /locus_tag="PK144_01500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNF64427.1"
                     /translation="MSRKIIHVDMDAFYAAVEQRDDPRLRGRPVVVGGDPRGRGVVAT
                     ASYEARRFGIRSAMPCAHAWRLCPQALFVRPRFEVYQSVSQQIRAVFARYTELIEPVG
                     LDEAYLDVSGVGVCRGSATLIARDLKIAVRAATGLVASAGVSYNKFLAKIASDLDKPD
                     GLAVITPEQGPAFIAKLPIGRFHGIGRATEQKMLALGIATGADLGAWSEARLVEHFGK
                     AGHWYAQLARGEDDRPVCAQRVRKSLGAETTYARDLGAAGEIEAALGALADEVATELG
                     RRGLAGRTLTLKVRYADFETVTRAHTLAPPARYAQAAELRTALPALLARTEACSRPVR
                     LLGLTVSGFPEEDGGEAESRGAAIGRMVQLPLF"
     gene            23712..24668
                     /locus_tag="PK144_01505"
     CDS             23712..24668
                     /locus_tag="PK144_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNF64428.1"
                     /translation="MPSIRPWRAVALAAALLAVAAVAPAAQFINVLTGGTSGVYYPLG
                     VALAKIFETAVPDSKPSVQATKASVENLNLLQAGRGELAFTLGDSLSQAWNGNEEAGF
                     RAPLKKLRTVAAIYPNYIQIVAAKESGIRTLADLKGRRISVGAPKSGTELNARAVLGA
                     AGLKYEDFDKVEYLPFGESVELMKNRQLDATLQSAGLGVASIRDLAQAVPIVVVAVPA
                     ETVGKIGDAAYRAAVIPANTYEGQTEDVPAVAVVNFLVTHEDVPEEVVYAMTKGMFEH
                     LDALAAAHAAGKSIKAETAASGSPVPLHPGAERYYREAGLLK"
     gene            24762..26783
                     /locus_tag="PK144_01510"
     CDS             24762..26783
                     /locus_tag="PK144_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNF64429.1"
                     /translation="MSAPPAPAPDRAARAIFAICIMFSVFQLWSAAFSPLPSQVVRSV
                     HVGFLLLLVYGLSAGRSGRTGAVLAWALGVAGFGLGLYHWVFLAELIQRAGEPSMTDL
                     VVGCAITVLVFDAARRQMGWALPLICAAFLAYALFGQYLPGHLAHRGYAFEQVIEQLV
                     LGTEGIYGIPTYVSSSYIFLFILFGAFLERAGMIDLFTDLAMGTVGHYRGGPAKVAVV
                     SSGLMGTINGSGVANVVTTGQFTIPLMKRFGYRADFAGGVEATASMGGQIMPPVMGAV
                     AFIMAETLNLPYVEICRAALIPALLYFATAFWMVHLEAGRAGLHGLAKADCPDAARAL
                     RERWYLILPLAALVWLLFAGYTPLFAGTLGLALTALLILGTPIALRLPGRALRIGFWL
                     ALGLAASAFLKFGITAVLALIAVLVAILAGLRGGRTTLALCVQALADGARHAIPVGIA
                     CALVGVIIGVTTLTGVASQFAGLIIDLGRDSLFLSLLLTMLACLVLGMGIPTIPNYII
                     TSSLAAPALLELGVPLIVSHMFVFYFGIMADLTPPVALAAFAAAPIAGASGLRIGLQC
                     MRIAIAGFVVPFMAVYTPALMLQGDPSVVAVLYIVVKALVSIGLWGAAAIGYLFAPLG
                     AGERGLAMAAAFLLVAALPVTDEAGFVLSAVFLLWHWRRTRQLPQMPPR"
     gene            26783..>27108
                     /locus_tag="PK144_01515"
     CDS             26783..>27108
                     /locus_tag="PK144_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HNF64430.1"
                     /translation="MPALCFGALPVAAGVFTLVWTHSVEHVRWAEDWRIDAGGRLQLV
                     EARISGSAAGMEPPAEARLAGGLWHYRPSLPPQPELRLTRSPFVADYTLCDATGCTAL
                     GARLPAG"
BASE COUNT         4009 a         9399 c         9422 g         4278 t
ORIGIN      
        1 cattcaaggc tttcggcgca ctggccgatc tgctgaagaa gcaggacggc tgaggaaagc
       61 gaacgggtcc ggggttgccg cgcttgccct tcaccccacg gctccgcgtc cagaaaccct
      121 gcgggagccg gtgaaaatcc ctcgttcagg tgcgatccgt gccagtcggg gtccggcctg
      181 ccggtgccgg ccgccgggtt cccgcggccg tcatttctcg atgtaccgat cccggtaggt
      241 gtgtgctgcg tcggcggacg gaagatcgtc ctccacggta tccgcggtcc tgatcaggcc
      301 cagattctcg aaggcgaacg acagcccgta ggctaccgac aggcgttgca gcgcagtttc
      361 cggcaactcg ggcgccacca ggttcgacgg acggttcagg tgcagcagcc tgagcgggta
      421 tcgatccgcg atcagccgtt cgatggcact gcggtacagg gcgacctcgc ggccgccgcc
      481 gcagagcagc acgggcaggc cgtcactcca ggcgcgcgaa ttgcggtaac ggcggccttt
      541 cgtctgcgtc agcacggcgc tgatcacccg gcagacatcg ttctcgaccg tgcggagtcg
      601 ctgctgcagt tccgcttccg tcacgccctt gattccggcc agcttctgcc ggtcaaagcc
      661 ggcatcctcg atcacacgtg ccgacgtgcc ggccagtgcc cggcggatcg cgtaatgaca
      721 gccgaggcgt tcaacgcggc tggcgaagat cgggaagacg tgctcgccgt cgctctcgtg
      781 gacgttgaag gtggatacgt cgagcgtgcc ggccccgaca tcgaccagca ggtgcaggtc
      841 gggccggcgc tgcggcgagt tcacgtacat cgcgatctgg gcgatgaatt ccggcacggt
      901 ggcgaggcgc tcaccgagca ggcacgggcg ctcctgctgc acggactgcg cggcggcgag
      961 tgtcggttcg tcctcggcga cgctgagctt ccaggctgcc tcgaccagtt gccggtattc
     1021 gtcgtcgatg ccggcctgtc gccagcgcgc cgcgggcacc ccgacgttca cgaaccactc
     1081 cagcctccgg ttcgcgtaga cgctgcgatg ctgcgtcagc agccagtggc gcgtatagcg
     1141 caggaccagg gcgagataac agacgattgc cgcacgggtc tgcgcatcca gcgtttcttc
     1201 gagcagccgg aacttcaggt cggcgagcag gcgattgttc cgatcgcaac cgagccagcc
     1261 gaggccgccg gagtcgatgg cgaggaagcc gggcaggaag caggcagctg caccgcgcgc
     1321 ccggaccgcg gcctcgaagt tcacgacgta gcggcgattc gcgccgtcgg caacgacgac
     1381 cttggtgaag gcggtgccga aatcgagtcc gatggtcatg cgctgccgcg cagcgcgggg
     1441 gccggcatcg agctgcagca gccggccgcc ggtgcgccag agtgccgtgt ccggctgccg
     1501 gggcaggcgc cgcggaacga tggccaggct ggagggcgcg agcgggtcgg tgatgggctc
     1561 tgctgtccgg gggacggacg cgggcgcggt ggctttgcgg gtcgtggcgt agaccgagag
     1621 gccacggccg tagccccgca gcggcggctt gccggagggc ggtgactcgg gtctggcggg
     1681 cgccggggat gctggagccg gcctggagga tgcggagcgg gctggcgacg ccggagtcag
     1741 ggccggcgcg ggcggggatg gtgacttcgc ggctgcggcc ggtcctgccg cggcagccgt
     1801 tgcgtctggc cgcgccgacg ttcgctgcgc cggcagggag ctgcgaatcc ggcggctcgt
     1861 tcccgggggc ggtgctgtct gccggcgcag gtctttgctg atggctgcca gcgcgcgctt
     1921 ccagtcctgc tcgtgatcgc tcatgcgtct tctcctgact ctttctccag atccctgatg
     1981 tctgtgtcca tcggtgttcg tccgtgtccg tgacgttcgt gcagcctatt cgatgcggat
     2041 cagtccccgg cacacctcca gccggtcgct cttgaggccg ttgcgctcgt tcaggcgctc
     2101 gcgctggatc gcaatgcgct cttcgagctt gcgccgctgt ccctcggtgg cgttgatgac
     2161 ccggcgcgtg cggccggcgg cccgcagctt tgccagacgt tcctcgatct ggtcgagctt
     2221 gcggcggcgg tggttttcca gcgcatcgag ctggaagcgg atacggtcat gattggccgc
     2281 ttccatggcg cgacgcttgc cggcataggt ctgcagcagg cgctcgaagc agtcctgcgt
     2341 acgctccgcc aggcccgcca ggtcgacgtc agcggccacc cccggccagt ccgcgccgtg
     2401 gtgcttggcg gcgttgacga gatccatcga cagatcggtt gccagcggcg catcggcatc
     2461 gacgggccag gcgagcgcca gcagttcctc ctcggtgcgc atcccggaga agctggtcag
     2521 cagcagcgca aacgcataga tgcccggtgc cggcagcgac ggcagcccgc tgcgctgccg
     2581 gtcgagccgc agtgccacca ccggccagac gctggtttcg aggcgctcga gttcctcgcc
     2641 gatgaaccgt ggcagcgggt ggaactggct gatcgtctcg atccggctgt ggctgctggc
     2701 gccgacccgg ttctcgaagc ggcagcggac ttcgtcgccg gtcccgagcc ggctttgccc
     2761 gagcaggtgc cggacctgca gatagttcgc atagcgcacg gcgaccgccg gcggcaggcg
     2821 caggtcgaat tcgaacggat cggcgccggg ctgatacagc gtgtggccgg gtgcgtgccg
     2881 ttcgagataa tcgcgcacat aggcggcgag gtccccggcg gtgatcgtct tgcggaaggt
     2941 gtgggcggcg ttgacctgtt cgaggatgta gccgccgtgc gcaatcatcg ttgaggcttg
     3001 gcgttcgagt tcgtcctgct gctggcgcac ctgctcgatc gcctgcgcgg tctggtcgat
     3061 gcgcagttct tcctcagcgg acgtcagctt ctgacagagc agatcctggg tcaggcgggc
     3121 gatctcgtcg cccagcacgt cctccaggct gccgagtgcg gtctcgaaca ggttcaggcg
     3181 ttcgtacagt cgccggacga tgcgctcgtc gatggtgccg gcataacaga ggttccagac
     3241 gaagatgctc tcggcgcgct ggccgaggcg gtcgatgcgg ccgatgcgct gctcgatgcg
     3301 catcgggttc cacggcaggt cgtagttgac gagcacccgg cagaactgca ggtccacgcc
     3361 ctcgcttgca acctcggagg acagcagcac gcgcgtctgc ggcgtgtcgc ggaagcggtc
     3421 gatgacatcc tgcttgctct cgcgcatgcc ccccatcagc acgcaggcgg acaggccgtc
     3481 atgctccagc cgtcgggcca gatagtgcag cgtcggacgg aagtatgaga acacgaccac
     3541 cttctcgtcc gggtgctccc gcagatactc gatgagcacc tcgtgcaggc gctggtactt
     3601 ggagtcgtgc cgggcaagct ccgcggtatc gaccgtgccg gcgagttctt cggcgatgcg
     3661 gcgcacgagg ggcgagcctt gcggccggtc gatgtcgagg gtcaggcact cgaacaggct
     3721 ctcggcgtcg agcgcgtctt cgcgccccag ccagctcgtg caggccgccg ccatcgaact
     3781 cgatacctga cgctgcggca tggcgagcag gaagccgtcg ctcacgtcgc gttgccacgc
     3841 atagccgcgc acggcatcgg tcacgctttc gtagagcgcg cgttccgcat cggtcggttc
     3901 gaccgcttcg acgcgcacgc gccgcacgac gcgccgctcc tgcacttcgg ccttgcgggt
     3961 gcgggtcacc gcgtgcgcga gcaggttcac gcgctcgatg cgattggcca gttcgatgcg
     4021 cgtcgcccgc gtatcgggtg caggcccgtc ggtgcaggcg gcgatcagat cctgcaactg
     4081 gcggctgccg gcgagcagcg gatgcgcctg cgccttgcgc agctcgtcga cgacctgcgc
     4141 gagactcgcc cgcaggtcca gcgcgcacgc gcgcgcccgc agcagcggac gattcgcgag
     4201 cagcacctgc tggaactgct cgccgtagcg gaagttttcg ggatcgacca gtcgcagcag
     4261 ctcgaacaga tcgtcggcgc gcgtgttgac cggggtggcc gacagcagca cgagatgcgc
     4321 gctgacctcg cgcagcaggc ggccgagttc ggcgctcaga ctctgcggat tgcgcaggta
     4381 gtgcgcctcg tcgatgacga ccagatcgaa caggggttcg gcgtcggcct gttcatcgag
     4441 caggcgtgcg agttgtgccc gctcgccctg ctcgtcggtg ctttgccagt cgccggaggg
     4501 ccgcagaccc tgcaggctgc cgaccagggc gcgctcggcg ggccaggtgt ccttcggctc
     4561 gcgcagcgcg gcgagcaatt caccggcacc gaccagttcg gcgcggatgc cgaagcgccg
     4621 gcgcagttcg tcccgccatt tttcacgcag catcgccggg cagacgacga gcagccggcg
     4681 tgcgtcgagg cgtgcgcgca gctcggtcca gatcaggccg gcttcgatgg tcttgccgag
     4741 accgacctca tcggcgatca gcaggccgtt gcacggtgat tccaggaacg agagcaccgg
     4801 cttgaactgg tgcgcgtaga agtcggtggc ggtggtatcg aggctgtaga tcaggttcgc
     4861 gagccggccc gagagctgga tgcgcgtcag ctgccggcgc agatcctcag tgcggccgaa
     4921 gcgaccggcg agcacgcgat cgtacggatc ggccggcgcg gcatcgaggg tttccagttc
     4981 gtaatcggca tagtactgac agccggccgg aaaccggacc tgtacccact gcacctcgcc
     5041 gcgctcacgg gtgagtccgg tgacgacgcc ggtctgaccg ggatcgtcga gcagacgcac
     5101 cggcatgccc gcctgccagc ggctcatgat cgggcctcct gctgcgcatg gcggacaggg
     5161 ccgcaaaccg gctgccggcg atcggggtag tgcgccgctg ctggtcgtga tagggccatg
     5221 ctggctttgc atccctcgat gcacatccgg tcgactccct gagcagatgt ccggcaatcc
     5281 ggctcatgaa aacgcgatta ttcttacaag tatatgatca tgatcaatta atcggcgagc
     5341 atgagtctcg cctgaccgca gcccctgcgg gagcggtgcc gcagggtggc tgcaaatgcc
     5401 cgatcagcgg ctccggatga cggctgcagg ccatgcagcc atgatccgcc ggaagcctgc
     5461 accatcaccc gaacacgcac ccggatgcac tggcaggccg tggcgcgcta cgctcgccgg
     5521 acttccgctg cccgcccagg cttaccgcag atgccgtcgc tccgtcgtcc gccgtccgct
     5581 ttccaccgcg cgcgccgcgc cgcgccggcc accgccctcg ccctgggcat cgccttgagt
     5641 ctcaccttcc cggttgccgc cgatgaggtc agccggccgc cggccgcccc cgatccgctc
     5701 tacgacccga agccgctcgc cgacgacatc gaactgccgc tgccctgcga cggccggctg
     5761 ctgctgcgca ccgtctacgt gctcgccaag agcgcgctcg atgaccgcga ggtcaacctc
     5821 ggctatccgt tcacgcagga cgaacccggc tacaagcagg gcttcgtcgc cggttaccgg
     5881 ctcggccacg tcaacggcca gttcgaaccg gccgacctgc cgacggcgtg gcgcacgcgc
     5941 ctcgacggca agctgccggc ggccggtgaa ggcgggctga agccgatgct gtacttcatc
     6001 ggcaagtacg agatcacgca gcgccagtac cggctggtga tggcgctgga gccctggctg
     6061 cacggtgccg cgcccgagcc gccggcctgt ccggcgccgg cggcgaccga ccggctgccg
     6121 atggtcgacc tgagctggtt cgacgcgctg aacttcacgg cgctgtacac cgaatggctg
     6181 atgcgcaagc accgcgaggc gctgccggcg ctcggcggcg gcgccggttc ggcggccggc
     6241 ggggcttcgg cctacgtgcg cctgccgacc gagaccgaat gggagtacgc ggcgcgcggt
     6301 gcgcaggtgg tcgacaaaca ggcgctggaa gcgcggctgt tcccgcgccg cgaaggcaat
     6361 gccagcgagg acgggccgct gcgcgactgg gcggtataca cgcaggtcgc cggcggcagc
     6421 ggccagacgg cgcggctcgc gccgatcggc acgaagcgcc cgaacccggt cgggctgttc
     6481 gacgtgatcg gcaacgccgc cgagatggtg ctcgatccct tcctgctggt gcgcggcggc
     6541 ggccggcccg gcggcggcac cggcggcttc gtggtcaagg gcggcaacta cctcgaaggc
     6601 gagcagacgc tgttcaccgg catgcgccgc gagtacccgc tgttcgacga caagggccag
     6661 ccgagccgca acacgacgac gggcttccgc atcgcgctcg gcacgctggc cgcaccgcgc
     6721 gcgcgctatg acgaactgtt cgccggctgg caggccgagg ggcgtctgag caacctgacc
     6781 agcgagctgc cgcccgacgc cgatgccggc gtcaagctcg accgcatcgt cgccgacctg
     6841 cagcgcgaca tgccgacctc gaccatcggc cgcgagctcg gcacggtcag cgaggaactc
     6901 aagcgctcgg tggcactgat cgccgcgcag cggcgcgatg ccgcggccaa cctgatccag
     6961 tcctcggcgc tggtcgccga aaccatcggc aactacaaca tccgcctgac caacctgaac
     7021 aagcagctcg aagaacagaa ggcgaagcgc aacgccgagg gcgtgcagtt cttcaccaat
     7081 gtcattgccg acggcaccag cgcggtgcag gcggcgctgt cgatctacct cgacaacgtc
     7141 aactccggcg ccgcctacgc cgaaaccctg ttcgccgagc agttccagcg cgtgcaggac
     7201 gagctcggca agaagcccgt gctcgggcgc agcctgagcg cgcgcgcgac gctgttcatg
     7261 aagcatgtcg agcagtaccg cgagacgcgc cggctcgatc gggaccgcgt gctgaacgaa
     7321 gtgctcggac gttaggaatc ctcaccctca tgcgcttcct ccttgccccc ctcgccgtcg
     7381 ccacgctggc cggctgctgc gtctgtgccc cgtcaccggc cccggtcgcg gccggcgacg
     7441 taccggtcgc ccccggcacg ccgccgctga ccgatttcta cgccgactgc ggccgcggcc
     7501 tgctcgatgc cgcgcgtctc gaactcgaac tcgaacgcga tgccgggcgc gcggcggtct
     7561 ggaacggcta catcgccgcc acgcagcgca cgcaggactg ctactgggcc gcgtaccagc
     7621 cgctgcttgc gcagcaccgc agcggcagcc tgaaccgcgt cgagggccgt gcgcgcctgc
     7681 gcgaactcgg cgacggcatt gccgcgaccc gcgtcgcgct cgcccgcaac ctgcgccggc
     7741 ccgaggccgt ggcgcgtgcc gccgacatgc agcgccagat cgcgcaggcc gacaatcgcc
     7801 tgcgcgagtt ctgcaccaat ccggatgtcg gcgactggct gccgccgggc ttcgactgca
     7861 ctaggctcag cgtctgaatc cggctgcctt cgcgatgccg gccgtgccgt gctcccggcc
     7921 agaaccagaa caacagaaca acagaacaac ctttacccgg agtcttcccg atgcatgccc
     7981 ttcctccgca gaccggtgcc cgcaggctcg ccgcggcgct gtccctgacc ctcgcgctcg
     8041 gcggttgcgc cggcgtccag ctcggcggcc cggcggatac cgtcgcgcag acgaccagcg
     8101 tcaactacta cccggcctgc tacgagccgg tgcgctacct gcgcgaatcc gacgacaaga
     8161 tgaaatccgc cgtcgccacc ggcgcggtcg gcggtgcctt ggccggtggc ctgctcggcg
     8221 cgctgaccgg cggtgacaac gccggacgca acgccctgat cggcgcggcc gccggcgcgc
     8281 tgatcggtgg cgccagcagc tattacctcg aaaagcagaa gcagaacgcc gaggacagcg
     8341 cgcgcttcgc cgcctacggc accgacatcg accgcgccac cggcgagtac aaccgcacga
     8401 cctcggccgc gcgcaacgcc cagggctgct accagaagga attccagacc ctggtgcagc
     8461 tgcgcaagac ccgcaggatc agcgacaccg agggccgggc gcgcttcaat gagatcgtct
     8521 cgggcatcag cgaggccaac gcgctgatgg ccgccgtcga ccagcgcatc ggcgagagcc
     8581 tcaacaccta tacgcaggcc tacgaggaag acctgaagaa gaccggcgtc gagcgtcagg
     8641 cggtcacccg cgccgcccgt tcctcgaagc cggtcgccgt cgccggctcc gccaagccgg
     8701 ccagcaccga agccgccgtc aacaccgagc gcaaggtgca gcaggcctcg accgcgcgcg
     8761 acgaatcgaa ggccgtgagc agcggcctga ccgcgcagct gaagagcttc tgttccaacc
     8821 ccgatgtcgg cgactggggc agtggcgcct cagcctgcgg caggggcgtc tgaatccgcc
     8881 tggaaccctc tgctgcccgg cacggaagca gcctgagaaa cccacctgca caggagtccg
     8941 taggccatgt ccctctctga accgaaaccc ggccagcgtc cgctggccgc cgccctgtcg
     9001 ctgctgctgg cgctgagcgg ctgcgcccag atgaacagca cgatgggcgt cgtgcagcag
     9061 accaccgtca actattaccc gaagtgctac gaaccggtcg gctacctgcg cgagtccgac
     9121 cagcgcatgc agcaggcggt cggcaccggg gtggcgctcg gggccctggg aggagctgcc
     9181 attggcgcga tagcgaccgg cgattcgcgg ggcgccatcg ccggcgcggt gctcggcggt
     9241 ctcgctggcg gcgccacggc ctactatcag cagaagcagc agcagcttgc cgaggacagt
     9301 gcccgctttg ctgcctacgg caccgacatc gaccgcacga ccggcgagtt caaccgcacg
     9361 acctcggccg cgcgcaacgc gcagcgctgc tatcagaagg agttcaaggc gctcgtgcag
     9421 ctgcgcaagc gcggcaaggt cagcgacgcc gaaggccggg cccgcttcgc cgagatcgtc
     9481 tcgggcatga gcgaggccaa cgcgctgatg gcggcggtcg acggccgtat cggtgaaagc
     9541 ctcgacacct acaccaaggc ctacgagtcc gacctgcaga agcgcggcgc gacgcgcagc
     9601 acggttgcgc agtccgccaa ccgtggcggc gggcgtggtt cgtcgaagag cagcggtgtg
     9661 ccgaccgagg cggtcaacgt cgagcgcaag gtacagaaag ccacggccgc ccgcgaggag
     9721 gtcaagaccg tcaccgccag catcgctgcc gacacgcgca gcttctgttc cagccccgac
     9781 atcggcgact ggggcaaggg caatgcgacc tgcgccaaga gtgcgtgagg ccgttcatac
     9841 cggtcccggc tgagcacgcc gtcttttcag cccctctccc ggtgcggggg aggggttgcg
     9901 gagggagctc aacaacacat tcacgtgcca cgtgtgagcg gcatgagccg ctggtgcagg
     9961 ctgccggcct cgtttgccgg atacaccgat ggtgcggctg gcgggcggat cgcgaacatc
    10021 ctgagcttcc ggaagccgca tgaagccgct tgcggctgcg atgcagcgtc acggcgaatc
    10081 ggtgtgctcg cgctcgggac tgccgagcag cgcgtcgagc aggccgagca gcgcgtcgca
    10141 ctgcgtgcgc agctcgccga ggcgcgcgcg cgccgcttcg gcctcgccgg ccaggtgcat
    10201 ggccagcagt tcggcgccga gggcgtggaa ttcttcgtgg ccggcatcga tcttcgccca
    10261 gaccgggtgc gtgccgtagc gctggcggcc ggggccgtgg taccagcggc cgaagcggca
    10321 gtcgtgcgtg ccgaacgccg gcgtgttgcc gatctcgccg ggcggcaggt ccagacaggt
    10381 gccgaggcgt tcgatccagc gctgcagttc gatgcgcgcc ccgagcagcg gcagctccgc
    10441 acgggtccag cgtgcggcgt gatgctgcca gagcgccggc ggctgccagc gtgccagcca
    10501 gtccggcagc gcgtcggccg gcatcggccg ggcgatgccg tagccctgcg catgcgtgca
    10561 gccgaaggcc agcagcaccg cgccgtgctc ggcggtttcg acgccttcgg cgaccacgcg
    10621 ccggtcgaag gcgcgcgcca gaccgatcac gccctcgacg atggcgaggt cgtcgctgtc
    10681 atcgagcatg tcgcggatga aggactggtc gatcttgacc acgtccgccg gcagccggcg
    10741 caggtgcgtc agcgacgagt aaccggtgcc gaagtcatcg agcgcgcagc cgacgccgag
    10801 cgcgcggcag tcggcgatga tctggcgcac gcgtgcgaca tcgccgagcg cggcgttctc
    10861 cagcacctcg atgcgcagca gccggcccgg cacgtgcggc tgcgcgcgca ggcgctgcgc
    10921 cagacgcgcg acgaagcccg gatgctgcag gtggtgcgcg gagacgttga cggcgaccgt
    10981 cagcgccagc cccgccccat gccagcgttc gagctgcgcg atcgcgctgt cgagcaccca
    11041 ttcgccgagc gcgatctcgg ccggactggc ctcgatcgcc ggcaggaagg ccgccggcga
    11101 cagcagcggc tgtccggccg gccgccagcg cagcagcgcc tcgacgccgg cgacgccgcc
    11161 gttgtgcatg tcgacctcgg gctggtagtg cagctcgaac tcgccggccg ggatgccggc
    11221 atgcacgcgg ccgatcagct cgctgcggtc gcgggcgcgg cgctcctggt cggcatcgaa
    11281 caggtgaaag ccgttgcggc cggcctgctt ggccgaatac atggcctgat cggcgtggcg
    11341 cagcagcgta tcggcgtcgc tggcatcgtc cgggtagacg gtgacgccga tgctggccga
    11401 caccgccagc acgcggccgt cgagcacgca gggcgtgcag accgccgcct gcagccgcgg
    11461 cagtgcaccg gtgcagtcgt cgcgctccgg caggccgccg agcagcagca cgaattcgtc
    11521 gccgccgagg cgcgcgaccg tgtcgccggc gcgcacgcag ccgagcaggc gctgcgcgat
    11581 ctcgatcagc agccggtcgc cggtctcgtg gccccaggtg tcgttgaccg gcttgaagcc
    11641 gtcgaggtcg aggtagcaga ccgcaagccg ggtgccggcg cgatcggcct gcgccatcgc
    11701 gtgcgcaagg cggtcggcga gcagcacccg gttcggcagg ccggtcagcg ggtcgtggtg
    11761 cacgaggtct tcgagccgct gctggtgttc cttcagcagc gtgatgtcgg tgaacacgcc
    11821 gacgtaatgc gtcaggcagc cggcgctgtc gtgcacggcc gagatgtcga gcagttcggc
    11881 gtacagcgag ccgtccttgc gccggttcca gacctcgccg cgccaccagc cgcgctcggc
    11941 cagcgtcgac cacatgcggt cgtagaacgc ggcgttctgg cgcccggagc ggaacacgcg
    12001 cgggttctcc ccgaggatct cgtcgcgcgt ccagccggtc agctcggtga aggcgcggtt
    12061 gacttcgagg atggtgccgg cggcatcggt gatgatgatg ccttcgtgcg cgtggtcgaa
    12121 cacgctcgca gccagccgca gccgctgttc ggcctcgcgc cgctcgctca cgtcgaccgc
    12181 gacgccgccg atgccggtga tgtggccggc ggcatcgcgg gtggggttgc cttcggtctg
    12241 cagccagcgc cagccgccgt cggcggtgcg cacgcgcagc tcgatcgtcg cccgctcgcg
    12301 ccgcccggcg gccagttcga agaacagcag gctggccggc gcgcggtcat caggatgcag
    12361 ccaggtgcgg aaatccgtgc cgagcgtcgc cgacagcgga tggccggtga tcgtggtcca
    12421 ggccggattg agcaggcgca cggtcgcatc cggtgcgatg tcgaacagca gttcgtgcat
    12481 gttctcgaac agcagccggt agcgcgcctc gctgtcggcg agcgccgact gcagggcgac
    12541 gcgctcggtg acatcgtgga cgatcaggta cagcagcgag cgtccgcgtg cgcgcaccgg
    12601 tgccgcatgc acctcgacat cgggctcgct gccatcggcc cgccggtggc ggcagaggaa
    12661 gtgcgtctgc gcggcagcca ccgcctcctc gatccggcta gcgagggctc cggccggcag
    12721 catgctcagg ccgctcaggt tcagcccgag cagctgcggc cgtgaccagc cgtagaagat
    12781 cgcggcggcg tggttggcat cgacgatgct gccgtcggcc ggatcgacca gcagcatcgc
    12841 gacgcggccg tcctcgaaga aatgccggta gtcgcgctcg taggcgcgca ggcgttcctc
    12901 ggcatcgcgg cgcacggtca cgtcgcgcgc gtagccgacg gtgccgatca cgcggtcatc
    12961 ggtgccgaac gccgggaact tcagggtctc gaaccagcgc cgctcgccgc gggcctcgat
    13021 cggttcctcg acctggcgcg gcaggccgtc gcggagcacg gccagatcgt cggcgcggta
    13081 gcgggcgcac agatcgggcg ggaagaagtc caggtcctcg tgcccgatca gcgccgccgg
    13141 gtccgcgtga ccggtcagca gcgccacggc gcggttgacg gcgacgaagc ggcagtcggc
    13201 atccttcatc cacaccggga acgggaagct gtcgagcagc gcgcgcagcc gcgcctgctg
    13261 gtcgagcgcg gcctcctcgg cctgcttgcg ctcggtgatg tcgcgggcga tgccgagcac
    13321 gccgagcagc tggccgttgc cggtgtgcag cggcgtcttg gtggtctcga tcaggcggcg
    13381 cgcgccgtcg ggatagacga tccattcctc gttgcgtcgc ggcacgccgg cggcggcggc
    13441 ttcggcgtcc ttgcgccggt agaacgccgc cgtgtcggca tcgacgaaat cgaagtcggt
    13501 gtgcccgagg atggcctcgg ccatctggcc gtaatagcgc tcgaaggccg gattgcaggc
    13561 cagatagcgg ccgtcgatgt ccttcagcca gaccatgtcc gggattgccc ggaacagcgc
    13621 ctgcagcagc gcggcgtcat cgttcatgtc cgcccccctt cggacccggt ggcgcacggc
    13681 ggccgcaatg gcccggaccg gcggaccgcc agcgtaggcg gcagcggcgg ctggcgatag
    13741 ctccggtatc ggccgccgcc gtcaattcct gaaacggcgc agggtcattg tgatccgccg
    13801 gcgcgctcgg cgtcgatctg gtcgagcact tcccgggcat tgcggaaggc ttcgacgccg
    13861 gccggcatgc cgcagtagac ggctgcctgc agcagcacct cgacgatctc ctcgcgcgtg
    13921 cagccgttgt tgatcgcgcc gcgcacgtgg cccttgaact cggtgccgcg gttgagcgcc
    13981 gtcagcatcg ccaggttgat caggctgcgc gtgcgccggt cgaggccggg ccgcgtccag
    14041 atctcgcccc aggcgtagcc ggtcacgagt tcctgcagcg ggcggctgaa gtcatcgagg
    14101 ttcgcgagcg cacgctcgac gaaggcatcg cccatcacgg cgcggcggac ttcgagacct
    14161 ttttcgaact gttccgggct catggcggta ctccgggtca agggacaggg gcagcaggga
    14221 cagtctggat gcggcggtgc agcatggtct tgacctgcat caaagcacag cccggcgccg
    14281 atcggcagac tcgcgcgtcc atcacgcttt ccgaatccgc ccgcctgcca ccgtttccgg
    14341 agtccacatg accgaagccg aatttgccga ctggtcgctg cgcctgctgc tgcctgccct
    14401 gatcggctgg atgggattca tcgtctgggg gctggcgcgc gagtccaagg ccggcaagtt
    14461 cggcaccttc gtgatgttcg gcgtgctcgg cctcggcgtg ctcggcttca tcgccaagaa
    14521 cgtcatcacc ttcatgctcg gactctgagc acgcccggcc acccgctgct tcaggcgatc
    14581 gtgtcgacgc agccgccatc gacgcgcagc gccgcgccgg tggtggccga ggcctgcggc
    14641 gagcaggcgt agacgatcag gttcgcaacc tcctcggcgc tcgcgagccg gcgcaggatc
    14701 gaactcggcc ggtgtgcggc gacgaaggcc gcaccgaccg cctcgatcgg ctgaccggtc
    14761 ttctcgactt cggcctgcag catcgcctgc acgccttcgg tcagcgtcgg gccgggcagc
    14821 accgcattga cggtgacccc gctgccggcc atgcgcttgg ccagcccgcg cgacaccgac
    14881 agcagcgcgg tcttcgtgaa gccgtaatgg atcatgtcgg ccgggatgtt caggcccgat
    14941 tccgacgaca ggaacaccac cctcccccag ccgcgcgtcg ccatgcccgg cacgtaggcc
    15001 cgcgccagcc gcacggcgga cagcacgttg acctcgaaga agcgctgcca ggtctcgtcg
    15061 ctcagctcga agaaatcctg cggaccgtag atgccgacgt tgttgacgag cacgtcgacc
    15121 gccggcagtg cggccagcag ctgcgcacag ccttcggcgc tgccgacatc ggcgctgatg
    15181 ccgctgacct cggcgcctgc gactgcggcg cggatcgccg cgcaggcctg ctcgacggcg
    15241 tcggcgtgcc ggccgttgac gatgacatgc gcgccgcagc cggcgagtcc gagggcggcg
    15301 gcatggccga tgccggcagt cgagccggtg acgagggcgg tacgtccgga cagatcgatg
    15361 tgcatggcag gaaactccgg tgcgggtcgc gaggacgacg ccggtccgac gcgcgatggc
    15421 ggggacggtt ctgcgcactt gcggactggc agcaaaaccg cagcggcata ccggccggcc
    15481 ggctgcgagc gtcgtcgtcg cagcctttcg agttatacac aaaccggacg ccggcggccg
    15541 gtttgtgcct gcagccgtgc atcgccgcag gcatggaaga caggtgtctg attgtcaagg
    15601 taatccggca cgggcgggcg aggccggcac gcggtccggc tgcggcggga cgggcggggc
    15661 tgacccgggg ttctccacaa aattatccac agggcggctg ccgcgcctgc cgctccggac
    15721 cgggcgcggg cgcggctatt tcctcttgcc gaagaagcgc ttgagggcac cgccgagcca
    15781 gcccgaggtt tcgggctcca gatccacctt gcccctggcc gtatgcactt gcacgacgct
    15841 ggaatagtcg ttgtcctcgc gcttgatgtg gttcacgagc cagttggtca gcgtgcggtg
    15901 cagttcttcg gtcacgtcat cgccgagctt gaagcggtcg tggtactcgt tgatgcggcg
    15961 gatgaacagt tcgtggatct tcttgtgcgc gttgacgtag ggatagcccg cctcctccat
    16021 cagcgtctcc tcgaaaccga agtggctctg cgtgtagtcg atcagatcct cgatgacccc
    16081 gccgaccaca tcgcggctgt gcttcaggcg cgcatcgtcg agctcgttca ggtaatcgac
    16141 gatgcgcttg tgctggctgt cgatgatctc gatgccgacg ctgagatcgt tgctccagat
    16201 gatatgggcc atctttccac gctccggttg tgtgacggtg aggccgggag gctccggcat
    16261 gagctgcgac ggtccgcgcc ggtatcccga ccggctgtgg ccgtcgcatg caatcgatgc
    16321 ggttttacgg ttcgtacgct tgttcgtacg cgggtgacgg gccggttcag tcgcgcagcg
    16381 cggccccgag caccagcgcg ccgccgctca ggccgagcgc ccaggtcacg aacggtgcag
    16441 cggcgaccac cgccgggttg aggctgttca gaccgtagac gacagccgcg ccgatcagca
    16501 gcgcggcgcc gaccgtggtc tggcggctgc gtgcgccctg gcggcggatg tcctcgcgca
    16561 gcgcatcgag cgagcgcgcg tgcacctgca tgcgcagttc gccgcttttc agcgcgtgca
    16621 gcagctcacc gatctgcacc ggcaggtccg gcaggtgctc ggcaagcttt ggcagattgg
    16681 ccttcagctt gttcagcagc gacttcgggc cgatgcgctc gttcatccag tcttcgagga
    16741 agggcttggc ggtcttccag aggtcgagtt ccggatcgag ctggcggccg aggccctcga
    16801 tgttcagcag cgtcttctgc agcagcacca gttgcggctg cacctccatg ttgaagcgcc
    16861 gcgcggtctg gaacagcgtc agcaggaagc cgccgaagga aatctcgcgc agcggccggt
    16921 cgaagatcgg ctcgcagacg gtgcggatcg ccgattcgaa ctcatcgacg cgcgtgtcgc
    16981 gcggcaccca gcccgattcg atgtgcagct cggcgacgcg ccggtagtcg cggcggaaga
    17041 aggcgaggaa gttctcggcc agatagtgct gatcgaccgg cgacagcgtg ccgatgatgc
    17101 cgaagtcgat ggcgatgtag cgcggatcgg ccggatcgct ggcatcgacg aagatgttgc
    17161 cggggtgcat gtcggcgtgg aagaagctgt cgcggaacac ctgcgtgaag aagatcgcga
    17221 cgccgcgctc ggagagcttc ttcatgtcga cgccgaggcg gcgcagctcc tcgatctgcc
    17281 cgaccgggat gccgcgcacc cgctccatga ccagcacctc gcgccggcag tacggccagt
    17341 ggatctcggg cacgtacagg atcggcgaat gctcgaagtt gcgccgcagc tgcgcgccgt
    17401 tggcggcctc gcggatcagg tccagctcgt cgaagatggt cttctcgtac tcggcgacca
    17461 cctcgaccgg ccgcagccgc cggccctcgc gccagtaccg ctgcgcgagc ccggcgacga
    17521 tgtacagcag ctcgatgtcg cggcggatcg tccgggcgat gccggggcgc acgaccttga
    17581 cgacgacttc gcggccgtcg aacagccgcg ccagatgcac ctgcgcgatc gaggccgagg
    17641 cgagcggctg ctcgccgaac tcggcgaagg tctcggcgag cggccggccg taggcctgct
    17701 cgatgatctg ccgggactgc tcgggcgcga acggcggcac gcggtcctgc agcttcgcga
    17761 gttcgtcggc gatgtcatcg ggcagcaggt cgcggcgcgt cgacagcatc tgcccgaact
    17821 tcacgaagat cggtccgaga tcctcgagcg tcaggcggat gcgctcgccg cgcgagcggt
    17881 gatcctcgcc gcgcagccag ttccacggca gcagatggcg cacgaagccg atcgggcgga
    17941 acagatgggt ggcgaggatg atgtcgtcga ggccgtggcg caccagcacc cggttgatgt
    18001 gcagcaggcg cagggcctgg gcgggacgca gcatgcgggg aaacccggtc agggaagcga
    18061 ggggggaagg tgaggccgcg gcgcaggcgt ggctcagcgg ctggcgaggc gggcttcgag
    18121 ccgcgcgagc cgcgcatcga gccggtcggc gtcggcgcgc agcgtgtcga tggcgtcgag
    18181 cagctcgtgc acgcgatcgg cgaccggcag cgcgccggcc tcctgctgga tgaactgcgc
    18241 gccgtcgtag aacagggttt cgcgtgcctg cgtcagccag ccgagcgcgc cgcgggcgaa
    18301 gcggccgagg cggtgcgcga gcacgtcgcc gaacaccttc gaggcctgct cctcccagtc
    18361 gatgtcgagc tcgcgcagca gcttctgcag cgcctggccg gcaagcggat caccgacgat
    18421 ctcgacgccg gcctcctgca ccggggcccc gcgcgcgagc agcaggaagg ccgccggcga
    18481 gccgcgcacc aggacctccg gcacgccgtg atggctgtgc acgacctgaa agccgtgcgg
    18541 caccggcacc agatacacca gcaggttcac gccatgcacc tcgaaggcga tggtcgcgcc
    18601 gcgcaggccg gcgagccgtg ccggtgcctc ggggtccatc gccagcaggc ggttcagcgc
    18661 ggtctcgatc gcggcggcga tcagcagcat cgggggcatc ggcgcgatcc ttcggaaacc
    18721 agtcatggcc gccgcgctcg gcacgggcgg cgcctgccgc gtcagtactt gtagccgcgg
    18781 tgcacggcga cgatgccgcc ggtcaggttg tagtactcga ccgactcgaa accggcggcc
    18841 tgcatcaggt cgcgcagcgt ctgctggtcg gggtgcatgc ggatcgattc ggccagatag
    18901 cggtagcttt cggcatcgtt ggcgaccagc cggcccatca gcggcagcat ccggaacgaa
    18961 tacaggtcgt agaccggcgc cagtccgggc acgaccggct tcgagaattc cagcaccacc
    19021 agccgcccgc ccggcttcag cacgcggtgg atcgaggcca gcgcccgttc cttgtgcgtg
    19081 acgttgcgca ggccgaaggc catggtcacg acatcgaagt acgcatccgg gaacggcagc
    19141 gcctcggcat cggcgagcac gtattcgacg ttgccggtga tgccctcgtc gagcaggcgc
    19201 ttgcgacctt cgtcgagcat cgaggcgttg atgtcggaca gcacgacccg gccgcgcgcg
    19261 ccgaccaccg acaccatgcg ccgggtcagg tcgccggtgc cgccggccag atcgagcacg
    19321 cagtcgccgc ggtgcaggcc ggcgagcgcg atcgcgtagc gcttccagag ccggtgcacg
    19381 ccgaaggaca tcaggtcgtt catcaggtcg tagcgggcgg cgaccgaatg gaacacgccg
    19441 gcgacacggc gtgccttgtc ctcgaccggc acctgctggt agccgaaatg agtggtggcg
    19501 gtgtcgtcgt taccgggcgt ttccgggctg gccatctcgc gtgctccgtc gggccggccg
    19561 ggcgaccggg cctgcgacct ggaaggattg ggaatgcggg tcctgatgcg gggctgccgc
    19621 ttttgcgctg cagcaggcac ggccctgcag catagccacg cggccccgcc ggctcaaacc
    19681 gccgtcaggg cccggcgcgg cgcacgtgac cggcggcagc gaggcgtgcg aggtactgct
    19741 gccagttcgc ggcctggtcg cgaccgagtt cgtacagcac gttccacgaa tacaggcccg
    19801 aatcgtggcc gtcggaaaac accagcttca ccgcatagtt gccgaccggc acgatgtcga
    19861 cgatctcgac ctcctccttg ccgacctgca gggtttcctg ccccggcccg tggccgcgca
    19921 cctcggccga cgggctgaac acccgcaggt attcgcagct catctcgaaa tgcgcgccgt
    19981 cctcgaagcg gacttcgagg atgcgcgtct gctggtgcag gcacagatcg gtcgggcgga
    20041 tgctcatggc ggcgctcccg gcggaggcgg gtgaagacgg aggcgcccac tgtgccacgg
    20101 caggcgccga tcggggcagg cacggattcg taacagaggt gtcgctgcaa ggcgacaatt
    20161 tgcacctgca gcatgcagga ggtgcagaat gccggccacg gccggtcgtg gtggccgcga
    20221 aggaccgatg catcacgcgg atgcaggcag aatcagacag atacgtcgct tttttacgtc
    20281 gctatgtcat cgacttgacg tgtcactgtc gcaaaagcgt caggggaatt gacagtacat
    20341 cagaacagcc atcggacgac attttgaaaa aacaaccact tgccgtgcga atgcctcgtg
    20401 gcaccaaagt tgcttttcat ccagtcgagc gttcctggcg tcgttccgcc gacccgaaac
    20461 gcggttccgc aggccccgtg gtcgcccctg gcgccatcct gccggatccg gatgttcagc
    20521 accctgaggt gctcgtcatg accaactaca tctcgttacg cgccgtctgg catgccctga
    20581 aagagcgcgg ctactggcgc atgtgcgccg cgatgtccac cgaccatccg gtcccggtca
    20641 gcccgaaccg ccgctgcctg tagcggcagc cccgtcccga tcccgcagtt cctcaccctc
    20701 cggtcgaccg gccgccgccc gctgcggcaa cccggcccga cctgccgctc cggcccgatc
    20761 tgctggccgg gcaacgcgcc gccggcggcg cgtgcgaggc ggcaccccgg cgcccgcagt
    20821 cgcgccggat ttccagcacg tccggtgaag cgccgatcgg cagcatcctc gcatccctgc
    20881 tgaagaccac ggctgtcgct ggtgtcggtg cgcaagcgtc cgcgaagggc ggtgcgtctc
    20941 cgcaggcgcg gttcagggca gatcgaaggc cgtgatcgtc tgcagggctg cgcagtcggg
    21001 atcggcgagg cgtcccaccc gcgccgcgtg tttttccagc gcctgctcga acaggttgcg
    21061 ggcgtgacgg gcgttgccga aggtgcgcgt cctgccggcc agcgattttt cgaacctgcc
    21121 gagcagcacc gaccgtgcct gcgcgtcgag gcggtaatcc ccgtcgatgg cgaagcgctc
    21181 gaagatttcg agcagctcgc cggccgtata gtcctcgaag tgcagcgtgc gccagaagcg
    21241 ggagcgcagg ccgggattgg catcgaggaa tgctttcatc tcctgcgtgt ggccggccac
    21301 gatcacgacc agccggtccc ggcggttttc catttcggca acgagcgccg caatcgcttc
    21361 gctgccgaag tccttgtcac tgttcggctg catcagcgaa tacgcttcgt cgatgaacag
    21421 cacgccgtcc atggccgcat cgcacttgcg tctgaccttc ggggcggtat ggccgatgta
    21481 ttcaccgacc agatcctggc gctggcattc gatgacgtgg cctttctgca gcagacccag
    21541 tcgccggtaa atgctgccga tccgccgggc gaccgtggtc ttgccggtgc cgggattgcc
    21601 ggtgaagatc agatgcagcg acaaagccgg cgttttcagg ccgacggcgc gccgggagtt
    21661 ctggacgcgg atgaactggg tcagcgattc cacctgtgcc ttgacgctgg cgatgccgat
    21721 gagatcggaa agatcatccg gtgccgatgc gtccgcttcg gcaggggggt catcctccag
    21781 ggggccgacc atgatctcgt ggcgctccag ttccatgatc gccccgggat gtccctgctc
    21841 ggccgccttg cgataccagt ggcgcgcctg ccggtcgtcc tgctcgactc cctggccctc
    21901 ctcgcacatc ctgcccagat tgcactgggc aaaggcctgt ccctgttcgg ccgccttgcg
    21961 ataccagcgc accgcctcct tgtcgtcatg aatgacgccg cggccatgct catacatcca
    22021 gcccagatcg cactgggcat cgggatgttc ctgctcggcc gcctggcgat accagtacac
    22081 cgcttcccga tcgtcccggt ccacgccaag accgtgtgca tacatccagc ccagattgcg
    22141 ctgggcatcg gcatcgccct gatcggccgc catgcgaaac cagcgcgcgg cttcgccatc
    22201 gttgcggctg acgccggtgc cgtgcgcata catccagccc acctcgaact gggcgtcggc
    22261 atcgccctgc tcggccaggc gcagattccg ctcgaactcc tcttcatctt catccgccat
    22321 ttcgacctcc atgctccggt ctgcgccgat gttttccggt cagccgatat tttatcgcct
    22381 gctgcgaacc tgcctgcccc ggatcgcagg ccggtgattt cattcccgtg ccccgcacgc
    22441 cctgagctgc cggagtgact gccgcttgtc gcgcaagatc atccacgtcg acatggatgc
    22501 cttctacgcc gccgtcgaac agcgcgacga tccgcgcctg cgcggccggc cggtcgtcgt
    22561 cggcggcgat ccgcgcgggc gcggcgtggt tgcgaccgcg agttacgagg cgcgccgctt
    22621 cggcatccgc tcggcgatgc cgtgcgcgca cgcgtggcgg ctctgtccgc aggcgctgtt
    22681 cgtgcgtccg cgcttcgagg tctaccagag cgtctcgcag cagatccgtg cggtgttcgc
    22741 gcgctatacg gagctgatcg agccggtcgg gctcgacgag gcctatctcg atgtcagcgg
    22801 cgtcggtgtc tgtcgtggat cggcgacgct gatcgcgcgg gacctgaaga tcgccgtgcg
    22861 tgccgcgacc gggctggtgg cctcggccgg cgtctcgtac aacaagttcc tcgccaagat
    22921 cgcctccgat ctcgacaagc ccgacggcct ggcagtgatc acgccggagc aggggccggc
    22981 gttcatcgcg aagctgccga tcgggcgctt tcacggcatc ggccgcgcca ccgagcagaa
    23041 gatgctcgcg ctcggcatcg ccaccggcgc cgatctcggg gcgtggtccg aggcgcgcct
    23101 cgtcgagcac ttcggcaagg ccgggcactg gtacgcgcag ctcgcgcgcg gcgaggacga
    23161 ccggccggtg tgtgcgcagc gcgtgcgcaa gtcgctcggg gccgagacca cctatgcgcg
    23221 cgatctcggc gccgccggcg agatcgaggc cgcgctcggc gccctcgccg atgaggtcgc
    23281 gaccgaactc ggccgccgcg gtctcgccgg gcgcacgctg acgctgaagg tgcgctacgc
    23341 cgacttcgag accgtcaccc gcgcccacac gctggcgccg ccggcgcgct atgcgcaggc
    23401 cgccgagctg cgcacggcgc tgccggccct cttggcgcgc accgaggcgt gctcgcggcc
    23461 ggtgcgcctg ctcgggctga ccgtctcggg cttcccggaa gaggacggcg gcgaagcgga
    23521 atcccgcggt gccgcgatcg ggcgcatggt gcagctgccg ctgttctgaa gcgcaccggt
    23581 gcggaccggc tgtggatcgg ccggccccgg cgcggcgggc tgggctatat ctgccggggc
    23641 aagcatcaat gacaagttgc ccggcccgcg cctgccgcag gccagaggca cccgacccga
    23701 ggagaatccc catgccgtcg atccgtccgt ggcgcgccgt cgcgctcgcc gccgccctgc
    23761 tcgccgtcgc cgccgtcgcg ccggccgcgc agttcatcaa cgtgctgacc ggtggcacca
    23821 gcggcgtcta ttacccgctc ggcgtcgcac tcgcgaagat cttcgagacc gccgtgcccg
    23881 attcgaaacc ctcggtgcag gcgaccaagg cttcggtcga gaacctgaac ctgctgcagg
    23941 ccgggcgcgg cgagctggcg ttcacgctcg gcgattcgct gagccaggcc tggaacggca
    24001 acgaggaggc gggctttcgc gcgccgctga agaagctgcg cacggtcgcg gcgatttacc
    24061 cgaactacat ccagatcgtc gccgcgaagg aatccggcat ccgcacgctc gccgatctca
    24121 agggcaggcg catctcggtc ggcgcgccga agtccggcac cgaactcaac gcccgcgccg
    24181 tgctcggcgc cgccggcctg aagtacgagg acttcgacaa ggtcgagtac ctgcccttcg
    24241 gcgagtcggt cgagctgatg aagaaccgcc agctcgacgc gacgctgcag tcagccggcc
    24301 tcggcgtcgc gtcgatccgt gacctcgcgc aggccgtgcc gatcgtcgtc gtcgcggtgc
    24361 cggccgaaac ggtcggcaag atcggcgatg ccgcctaccg cgccgccgtg atcccggcca
    24421 acacctacga gggccagacc gaagacgtgc cggcggtggc ggtcgtgaac ttcctggtca
    24481 cgcatgagga cgtgccggag gaagtcgtct acgcgatgac caaaggcatg ttcgagcacc
    24541 tcgacgcgct cgccgccgcg cacgccgccg gcaaaagcat caaggccgaa accgccgcca
    24601 gcggctcgcc ggtgccgctg catccgggcg ccgagcgcta ttaccgcgag gccgggctgc
    24661 tgaagtaaac cttgccgaag cgggcgccgc cggtgcttgc ggcagcgcca gtccccgtcg
    24721 ccgctgccgt ccggattccg cccttcatgt ccgcgccccc catgtccgcg cctcccgctc
    24781 ccgcgccgga tcgcgccgcg cgcgcgatct tcgcgatctg catcatgttc tcggtgttcc
    24841 agctctggag cgccgcgttc tcgccgctgc cgagccaggt cgtgcgctcg gtgcacgtcg
    24901 gcttcctgct gctgctggtc tacggcctgt cggccggccg cagcgggcgc accggcgcgg
    24961 tgctggcgtg ggcgctcggc gtcgccggct tcgggctcgg gctgtatcac tgggtgttcc
    25021 ttgccgagct gatccagcgc gccggcgagc cgtcgatgac cgatctcgtc gtcggctgcg
    25081 cgatcaccgt gctggtgttc gacgccgcgc gccggcagat gggctgggcg ctgccgctga
    25141 tctgcgcggc cttcctcgcc tatgcgctgt tcggccagta cctgcccggc catctcgcgc
    25201 accgcggcta cgccttcgag caggtcatcg agcagctcgt gctcggcacc gagggcatct
    25261 acggcatccc gacctacgtg tcctcgtcgt acatcttcct gttcatcctg ttcggcgcct
    25321 ttctggagcg cgccggcatg atcgacctgt tcaccgatct ggcgatgggc acggtcggcc
    25381 actaccgcgg cgggccggcc aaggtcgcgg tggtgtcctc ggggctgatg ggcacgatca
    25441 acggttcggg cgtggcgaac gtcgtcacca ccggccagtt cacgatcccg ctgatgaagc
    25501 gcttcggcta tcgcgccgac ttcgccggcg gcgtcgaggc cacggccagc atgggcgggc
    25561 agatcatgcc gccggtgatg ggcgcggtcg ccttcatcat ggccgagacg ctgaacctgc
    25621 cttacgtcga gatctgccgc gccgcgctga tcccggcgct gctgtacttc gcgaccgcgt
    25681 tctggatggt gcacctcgaa gccggccgcg ccggcctgca cggtctggcg aaggccgact
    25741 gccccgatgc cgcccgtgcg ctgcgcgagc gctggtacct gatcctgccg ctggcggcgc
    25801 tggtctggct gctgttcgcc ggctacacgc cgctgtttgc cggcacgctc gggctggcgc
    25861 tgaccgcact gctgatcctc ggcacgccga tcgcgctccg gctgcccggg cgggcgctgc
    25921 gcatcggctt ctggctcgcg ctcgggctgg ccgcctcggc cttcctgaag ttcgggatca
    25981 cggcggtgct cgcgctgatc gccgtgctgg tcgcgatcct cgccgggctg cgtggcgggc
    26041 gcacgacgct cgcgctgtgc gtgcaggcgc ttgccgacgg cgcgcgccac gcgatcccgg
    26101 tcggcatcgc ctgcgcgctg gtcggcgtca tcatcggcgt gaccacgctg accggcgtcg
    26161 cctcgcagtt cgccggcctg atcatcgatc tcggccgcga cagcctgttc ctgtcgctgc
    26221 tgctgaccat gctcgcctgc ctcgtgctcg gcatgggcat cccgaccatc ccgaactaca
    26281 tcatcacctc ctcgctcgcg gccccggcgc tgctcgaact cggcgtgccg ctgatcgtct
    26341 cgcacatgtt cgtgttctat ttcgggatca tggccgacct gacgccgccg gtggccctcg
    26401 ccgccttcgc ggcggcgccg atcgccggcg cctcgggcct gcgcatcggc ctgcagtgca
    26461 tgcgcatcgc catcgccggc ttcgtcgtgc cgttcatggc cgtctacacc ccggcgctga
    26521 tgctgcaggg cgatccgtcc gtcgtcgccg tgctctacat cgtcgtcaag gcgctggtgt
    26581 cgatcggcct gtggggcgcg gcggcgatcg gctacctgtt cgcaccgctc ggcgccggcg
    26641 agcgcgggct ggcgatggcg gcagcctttc tgctggtcgc ggcgctgccg gtcaccgacg
    26701 aggccggctt cgtgctgtcg gccgtgttcc tgctctggca ctggcggcgc acccgccagc
    26761 tcccgcagat gcccccccgc tgatgccggc gctctgcttc ggcgcgctgc cggtcgccgc
    26821 cggcgtgttc acgctggtct ggacgcattc ggtcgagcac gtgcgctggg ccgaggactg
    26881 gcgcatcgac gccggcgggc ggctgcaact ggtcgaggcg cgcatcagcg gcagcgccgc
    26941 cggcatggag ccgccggcgg aggcgcggct cgcaggcggc ctctggcact accgcccgtc
    27001 gctgccgccg cagccggaac tgcgcctgac gcgctcgccc ttcgtcgccg actacacgct
    27061 gtgcgacgcc accggctgca cggcactcgg ggcgcgacta ccggccgg
//
LOCUS       DAOQIE010000012        26053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_823_length_26053_cov_6.752096, whole genome shotgun sequence.
ACCESSION   DAOQIE010000012 DAOQIE010000000
VERSION     DAOQIE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_26053_cov_6.752096"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK144_01520"
     CDS             <1..793
                     /locus_tag="PK144_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HNF64431.1"
                     /translation="NFVTSHDGFTLADLTCYRERHNHANGEDNRDGHGDNHSDNHGVE
                     GDTADGALLAARRRTRLNLLASLLLAQGVPMLLAGDEFGQSQAGNNNGYCQDNALCWL
                     DWSRLETEAGFTAAVRRLTALRREFAVLRSRCWLHGVHGDDARPAPDELRWLGPHGGD
                     MSIPAWEDPHGRCLGLWLHGTDGRELIVLLNAGPAMDFRLPANAGDGWQVAFDSSAFE
                     AAPGPAAVVHALPARSLRVLSRVPAGTAPASSDSPPVPGRARKPG"
     gene            828..2096
                     /gene="glgC"
                     /locus_tag="PK144_01525"
     CDS             828..2096
                     /gene="glgC"
                     /locus_tag="PK144_01525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNF64432.1"
                     /translation="MENQTATPRYVSRLTRETMALVLAGGRGTRLYELTQWRAKPAVY
                     FGGKFRIIDFPLSNCVNSGIQRIGVLTQYKAHSLIRHLIAGWGSVLSRELGGGIEILP
                     ASQRTSDDWYAGTADAIYQNLDILRVRDPSYILVLSGDHIYKMDYGPMIAAHVESGAA
                     MTVSCIEIPLAEASSFGIISVDAASRVTAFNEKPQQADPMPGRTDVALGSMGNYVFNT
                     EFLFEKLIEDAKDPNSSHDFGKDVIPAIIRDHHVHAYLFRDPATGRLGYWRDVGTVDA
                     FWEANIELAAIQPELNLYDHHWPILTYQSQLPPAKFVFEEPGRTGSAPNSLVSGGCLI
                     SGATVRHSVLFSNVRVMDHALVEDAVILPDVVISPGARIRRAVIDRGCTIPEGMVIGE
                     DLTQDAKQFRVTQRGIVLVTKEMLGQGRVP"
     gene            complement(2078..3079)
                     /locus_tag="PK144_01530"
     CDS             complement(2078..3079)
                     /locus_tag="PK144_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNF64433.1"
                     /translation="MPVLPFTASADVKPRLIRFGYGLAEDSNQGRAARFFAEEVGRLS
                     GGRLRVRTFGGASLGTDIQMQNALIGGAQEMMVGSTATLVGIVRDFGVFDLPFLFDDE
                     RIADAVLDGPFGTRLLDTLPAQGLIGLVYWENGFRSVTNTRRPIERLEDLHGLKLRVM
                     QNPVYLDFFGRTGANPVPLPFSELFTALETRAVDGQENPVNTIRSSRFYEVQKYLSLT
                     RHVYSPWILLVSRLWWDKLSGDERALLREAAVAARDFERRDSRAEAGQALAQLQAQGM
                     LVNTPSTAELERLRAAVAPVVEAWAAQASPGLVAQLRQAIDAARAAQEPQAAQGTRP"
     gene            complement(3252..4529)
                     /locus_tag="PK144_01535"
     CDS             complement(3252..4529)
                     /locus_tag="PK144_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNF64434.1"
                     /translation="MTLLLFLAVLFGALALGMPIAFALILGGAALMLQLEVFDAQLVA
                     QNLIAGVDSYPLMAVPFFILAGELMNAGGLSRRIVALAIAGVGHVRGGLGYVVIATAM
                     LLASLSGSAIADTAALTTLLLPMMRAHGYPLAGSAGLIAAGGIIAPIIPPSLPFILYG
                     VSTSTSISSLFLAGIVPGLLMGVVLVLVWRRRLAGFGCIPQPPAPARERRRALLEAGW
                     ALLLPVLILGGLRGGWFTPTEAAVVAAVYALVAGCVLYRELDRGALYSAFVRSARTTG
                     VVMFLCAAATVSSYMITLAELPGQLAGPLAPLLAHPRLLMAVIVGLLLLVGMVMDLTP
                     SILILAPVLLPLARSAGIDPVYFGVMFVLVGSIGLITPPVGTVLNVVGGVGGVPMLQT
                     VRGVLPYVGAYCALLGLFVLFPGLITAPLQWLR"
     gene            complement(4526..5092)
                     /locus_tag="PK144_01540"
     CDS             complement(4526..5092)
                     /locus_tag="PK144_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNF64435.1"
                     /translation="MKPLCSQRPGGATEALMVVALALMVALVFGNVVARHVFSTGFAA
                     AEELARLLFVWLVFLGAIVALREQRHLGLDLLQARLPGPLRRLCAVISHALMLWALAL
                     FAEGSWQQSVIGLRTYSTVLGFPQLLVAGSGLVSALAMAGLVARNLWWILAHDPRACV
                     PGQPAVADDSDSDSDSDSDAHGRTGTGQ"
     gene            complement(5128..5640)
                     /locus_tag="PK144_01545"
     CDS             complement(5128..5640)
                     /locus_tag="PK144_01545"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNF64436.1"
                     /translation="MATNIVIMGVCGAGKSLIAGRLAAALGLPHIEGDAFHTCANIAR
                     MSAGVPLTDAERVEWLDQLAGQLGAAALAGSGAVLSCSALKQCYRERLRRGCEGYGLQ
                     FVYLRGSREQLAERLAHRHGHFMPVTLLDSQLADLEEPLPDERAIVCDITATPAQIVA
                     GIVDRLSAQA"
     gene            5885..6910
                     /locus_tag="PK144_01550"
     CDS             5885..6910
                     /locus_tag="PK144_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNF64437.1"
                     /translation="MPPRKPHSRASGRVTLADVAARAGVSAISVSRTLQSPAQVSAAL
                     RARIQAAIAELGYVPDHAARSLASARSGTVVVLVPSLGNAVFVETLTAIQAVLQPRGW
                     QMLIGNTRYSPAEEEALLLAWLGHRPDGILLSGLDQTGRTRALLAGSGAPVVHMMEID
                     ATGACCSVGSGQEAAGAAMAGHLLARGYRRIAFIAVQLDPRTRQRREGWRQVMRDAGC
                     YEPARDLESPQPSSVGLGVEMLERLLAQAPDSDAVFCCNDDLAHGVLFECQRRGIDVP
                     GRLAVAGFNDLPASAWTHPRLTTIAIPRTGIGQEAARLLTELMAGRTPAQRTIDLGFE
                     LKARDST"
     gene            complement(6944..7750)
                     /locus_tag="PK144_01555"
     CDS             complement(6944..7750)
                     /locus_tag="PK144_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF64438.1"
                     /translation="MRLLILSDLHVDAHRAAARHRGCEASPWPPLPLEGVDAVLCAGD
                     VANGPEGLAELAPRCRGVPLIAVAGNHEYYAGPADTGAVQARLQALSDAGVLHWLERR
                     SLLLDGLRILGTTLWTDFALYGDAGAGMAACAVVMQDFRGAIRQAGRRLEPADTLAWH
                     RRDRDWLAAELARPFPGPTVVLTHHAPHPDCIEPFYAGDPVSTGFASDLSALFDGRIA
                     LWVHGHCHRATDFRIGGTRIVSNPRGYPDEPAAQTGFEPGRIIDLPAAAG"
     gene            complement(7758..9128)
                     /locus_tag="PK144_01560"
     CDS             complement(7758..9128)
                     /locus_tag="PK144_01560"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HNF64439.1"
                     /translation="MNRYLAPAALLPEGWARRVLLEVDTHGCLGRVEPDCPPAQAEGA
                     EVLAGPVLPGLANLHSHAFQRALAGLAQRAERRDESFWSWRERMYALVAHLSPEDIQT
                     LARRVYIELLKAGWTAVAEFHYLHRDARGRAYADPAELALRIVAAARETGIGLTLLPA
                     LYRLGGFGERPVAGAQKRFAIGVDDFLHLLERVREHGAREGFVTGTALHSLRAVAAAD
                     MRTVLQTLPRASVIHIHIAEQRREVDECLAWSGRRPLDWLFAHAPVDARWCLVHATHV
                     DATELARLAGSHAVAGLCPSTEADLGDGLFPATEYGALNGSFGIGTDSHVRVDPAGEL
                     RLLEWGQRLRLERRACLASPGQPSVGGFLLRRALAGGAQACGQPTGALAPGRRADWVV
                     LDGAQPLLAGAHGDAITERWIFGGDSRFVRDVMVGGRWCVREGHHPLEAQADAAFASV
                     IARVTA"
     gene            9262..10941
                     /locus_tag="PK144_01565"
     CDS             9262..10941
                     /locus_tag="PK144_01565"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNF64440.1"
                     /translation="MQTRHDPDRLIRAPRGPGLRCRNWQAEAALRMLMNNLDPEVAER
                     PSELVVYGGIGRAARDWSCYDRIVATLERLTDEETLLIQSGKPVGVFRTHAEAPRVLL
                     ANSNLVPHWANWEHFNALDRAGLMMYGQMTAGSWIYIGSQGIVQGTYETFAAMADAHF
                     GGEARGRWILTAGLGGMGGAQPLAATMAGYSLLAVECDPARIEARLRTGYLDRAAGTL
                     DEALALLDAARAAGTPVSVGLPGNAAEVFPALLARGVQPDIVTDQTSAHDPLHGYLPA
                     GWTLAEWQARQQSGPEAVIAAACASMAVQVRAMLGFRARGAAVVDYGNNLRQRALDAG
                     VEDAFAYPGFVPAYLRPLFCRGIGPFRWAALSGDPADIARTDALVRELIPGDAHLHRW
                     LDLAAARIRYQGLPARICWVGLGDRHRLGLAFNALVRSGELKAPIVIGRDHLDSGSVA
                     SPNRETEAMRDGSDAVSDWPLLNALLNCASGASWVSLHHGGGVGMGYSQHAGVVIVCD
                     GSAAADRRIARVLWNDPATGVMRHADAGYPEAIACARAHRLDLPMLGGDEA"
     gene            10938..12209
                     /gene="hutI"
                     /locus_tag="PK144_01570"
     CDS             10938..12209
                     /gene="hutI"
                     /locus_tag="PK144_01570"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNF64441.1"
                     /translation="MSALPYAEALWLGGPIATLAPAGDDPWGLLPDGAIAVSDGRIVW
                     VGAASELPGGFTAAEVHDLGGRWLLPGLIDCHTHLVWAGSRVEEYERRLAGASYEDIA
                     RAGGGILATVQATRAADAGALYAAAAARLADLCADGVTTVEIKSGYGLDVGTERRQLE
                     VARRLGQEQPVNVYTSFLGAHVVPPEYSGRAGLWIAFLADELLPMLAADGLVDAVDAY
                     CEPFAYTPSHCETLFAAARALGLPVRVHAEQRSRSGGARVAARFAALSADHLEYADES
                     DIAALAAAGTVAVLLPGAFLTLGERQVPPLAALRRHGVPIALASDLNPGTSPLRSPRL
                     AAALGCQLWGLTPAEAIAGLTRHAARALGLAATHGTLEPGKIADFSIWKVEHPAELAY
                     WLGGQICTGRVLAGRPVAAEADRCLSIPSTT"
     gene            12182..13003
                     /gene="hutG"
                     /locus_tag="PK144_01575"
     CDS             12182..13003
                     /gene="hutG"
                     /locus_tag="PK144_01575"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HNF64442.1"
                     /translation="MSFDPVYDLIPGDQPLLISIPHGGTRIPDALRPKLAPLAQALPD
                     TDHHVPRLYAFAPELGIGVLAARWSRYAVDLNRPADDAPLYESNTTTTGLFTDECFDG
                     SPLLLPGERPDPWLRADILDGIWRPYHACLAHELARLKDRFGYALLWDAHSVRSVLPR
                     LFEGRLPDLCLGTNDGQSCDPELTARLVGICAAAHRYTQVLDGRFKGGWITRHYGQPH
                     AHVHAVQMELSQATYMDEDAPWAFREDLAADLVPVLRTLLETMLVWGRERYGASS"
     gene            complement(13144..13524)
                     /locus_tag="PK144_01580"
     CDS             complement(13144..13524)
                     /locus_tag="PK144_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF64443.1"
                     /translation="MEIRPVRTESDYRATLAEIETLMNAGAGTPEGDRLDVLVTLVEA
                     YERQHYPLDFPDPVEAIKFRMEQQGLTVDDLVPVIGRKNRVYEILARKRPLTLRMIEG
                     LHRVFSIPAESLLKRSDRRHQPVA"
     gene            complement(13549..13851)
                     /locus_tag="PK144_01585"
     CDS             complement(13549..13851)
                     /locus_tag="PK144_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNF64444.1"
                     /translation="MRVIALSTLKAFWSASPTHADAIGPGLAWYRETLRADWRTPNDV
                     KAQFASASILRDGRVVFNIAGNKYRLVVWINYAHRVVYIRFIGTHAQYDGIDAQTI"
     gene            13984..15558
                     /locus_tag="PK144_01590"
     CDS             13984..15558
                     /locus_tag="PK144_01590"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF64445.1"
                     /translation="MTPTPLHILMVAAENDALPGAKVGGIGDVVRDLPPALAVLGCTV
                     TVVLPGYGFLAHRPGAHTLTRLPVGFGGTRTEVELFEVPARHAQAGVRHIVIEHPAFA
                     HGGRIYCDDPPDRPFATDAGKYALLCAAVAEATVNGQFGHVDVLHLHDWHAAFLTILR
                     RFHPGFAPLRALRCVVTLHNLALQGVRPLRGHASSLEAWYPGLPWEPWAVLDPRWLDC
                     VNPMAAAIRLADAVHAVSPSYAQEILLPSDVAGAGRYGGEGLEVDLQRARDEGRLWGI
                     LNGCEYPAPMPPPPEWPALLKLARGEVLRWAGAAGGPAVASAHFLAHARLGDWLERPR
                     PATLLTSVGRMTDQKAALLLERSSDGRSALDAMLDALGPDGAWISLGSGDPAYERALV
                     AASARHPNFVFLQGYSEPLSSALYAAGDLFVMPSSFEPCGISQMLAMRAGQPCLVHAV
                     GGLRDTVEDGVTGFAFAGDGRSAQADALVLALYRALDARREPGRWQAMSTAAREARFL
                     WEDSARACLVHLYHAG"
     gene            15598..16533
                     /locus_tag="PK144_01595"
     CDS             15598..16533
                     /locus_tag="PK144_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF64446.1"
                     /translation="MEDPSAVPRSHADRPLAAGTGKLGGAPVRKRRLRRGLLLALALY
                     ALYAAGFWHWQRALLYPGTARAADPEAARRFPDAQVEWIPLGEARIETWYLPPLPKPG
                     HDPAARAPALIVAHGNGELIEDWPRRFEPFRRLGLGLLLVEYPGYGRSGGTPDEAGIR
                     RLFAAAWDRLAARPDIDPARIGGLGISLGGGAIGTLIPQRPLAAVILLSTFTDIGAFA
                     GRYGLPRDLVLDPYDTRAALAGYDAPVLVIHGRRDGVIPYAHAQALAAAGRHATLHLH
                     DCAHVCWEPERLPLWAELEAFLRRTPLLPASVHQD"
     gene            16791..17780
                     /locus_tag="PK144_01600"
     CDS             16791..17780
                     /locus_tag="PK144_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNF64447.1"
                     /translation="MPSLATQYLGLKLANPLILGASPLGHEPLLAQRVADAGIGAIVM
                     HSLFEEDLLEDVTQRQLLGLPTDPDSQRALFRGSLDRYLKQLEKLKKSVGVPVIASLN
                     GVSASGWMEQAKALVDAGADALELNVYYLAASPAESSTAVEARYIELVRQLRATLRIP
                     FAVKLSSQFTALPHFLRRLEAEGASGFVLFNRFYQPDIDLNGVRLIHRPIRSRSEDVL
                     MALHWIAMLRGRINGGLSASGGVQHADDVLKLLLAGADTVQIVGAVLNEGPGLVSDIL
                     AGVGEWLDAHGYDSVADLRGRFSENRISDPGAYERIHYRGVLDNWRAPGEAWR"
     gene            complement(17818..18807)
                     /locus_tag="PK144_01605"
     CDS             complement(17818..18807)
                     /locus_tag="PK144_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNF64448.1"
                     /translation="MQSLDLDVLASAAGWRRSGEAVALATVLATYGSAPRPPGAIAAI
                     RVRDGAIAGSVSGGCVEEELVARLARTGPPALPQRIVFGATREEAVRLQLPCGGTLHL
                     LLETLTDPAWIDAVLATTAAGGHLLRELKLASGAQRLVPADATTAFSGDGPLLRQPFG
                     PRWRLLLIGATDVAALLAPMAQALGYAVAVCDPRPGHAGQPLAEGVTRLGGSPDDAVL
                     AFAPDAHSAILTLAHDPRLDDLALMEALASPAFYVGALGSQRTSQARRARLAEVGVEA
                     AAIERLHAPVGLAIGSRTPAEIAVSILAELVAVRARLGQGGAQPQAAAPELAA"
     gene            complement(18822..20993)
                     /locus_tag="PK144_01610"
     CDS             complement(18822..20993)
                     /locus_tag="PK144_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNF64449.1"
                     /translation="MSLDVSISRRGFLKGGAMLGAGLVLAFHLPATLKRLQAAQAADA
                     PAKEYPPDAFIRIAPDGTTTILCNKAEMGQGVYTSLPMLICEELEADWSQVRVEPAPA
                     AIVYAHPVFGIQMTGGSTSVASSWEQMRRIGAVARTMLVETAAARWNVPAVDCRAENG
                     FVLHGDRKLSFGELAEEAGRRTPPASVTLKNPKDFKLIGKATPRLEGPDKVSGEGQFG
                     IDVRVPGMLFAVLARPPVFGGKPKQWDSAAAQAVPGVLAVKAVAGGVAVYASNTWAAL
                     KGREALKVQWDDGPNAGFSTAAQRAGFQKLAREPGTVAVAQGDAPGALKTAAKTLDAE
                     YEVPYLAHACMEPLNCTVRVDADAVEIWAGTQFQTVDQGNAAKIAGVKPEQVTIHTTL
                     LGGGFGRRANPASDWSNEAVEVAKAMPGKPVMTVWSREDDTRGGWYRPHWVSRLRAGL
                     GADGLPVAWLHTIVGQSILAGTPFEAFMVRGGIDATSVEGASDLPYAVANLQVDLHSP
                     KSPVPVQWWRSVGHSHTGFVVESFVDECAHAAGIDPLEYRRRLLAKHQDPRFLKCLNL
                     AAAKAGWSRPPEKGSGRGIAIHESFESVVCEIAEVTQKDGRVQVTRVVAAVDCGTAVN
                     PDMVRAQIESGIVYGLSAALYGQITLKDGRVEQGNFDDYPAVRLSESPQIEVHIVPSE
                     APPTGVGEPGTPPIAAAVGNALFALTGERHRRLPLMPVAKS"
     gene            complement(20997..21461)
                     /locus_tag="PK144_01615"
     CDS             complement(20997..21461)
                     /locus_tag="PK144_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNF64450.1"
                     /translation="MTTVEVNGQSREIDAPGDMPLLWALRDVLGLTGTKFGCGRGLCG
                     ACTVHLDGAPVRACLTPLSAAAGRKLTTIEGLAPAGGLHPVQQAWLDEGVVQCGYCQS
                     GQIMSAAALLASNPKPSDADIDNAMGGNLCRCGTYGRIRAAIHRAAGAERSA"
     gene            21648..22268
                     /locus_tag="PK144_01620"
     CDS             21648..22268
                     /locus_tag="PK144_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF64451.1"
                     /translation="MNPCPPADRRSRRRERTAEDILAAAVALFAEQGWAATTMEQIAA
                     RADIAKATLYSHFPVKEAILVAQMQQELRMQWPAFEQRIHETTGTRAQLLALFELAAP
                     WLEAHRDVMAPYVAYRLSRPRGDGSQRSGMDKLFTAIVERGRARGELRDDLPVAVLVM
                     HLQFAYLAELLRWLEAPEAGHELGAALAAMAGLFLDGAAQRPEQPS"
     gene            22265..25075
                     /locus_tag="PK144_01625"
     CDS             22265..25075
                     /locus_tag="PK144_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNF64452.1"
                     /translation="MSASDRTETRLEAAPRITLLDPAAARAQGMDSAGGKGWQLARMA
                     HYALPVPDFRVLPAAAALAAVEATGLDADAADAGDDPAALAAVAAALSSGEPPAWLQA
                     VVDAVLALPGFDRPLAVRSSARCEDSATASFAGIHDSKLNVPPDAPAVCAAVRAVWAS
                     AWSAHAQAYRRRLGLDWRAAALAVVVMPLVPARAAGVAFSCDPQTGATDVIVIEAVTG
                     LGEALVGGQVEPDRWRLRLPAPGHAALIADTRIGRKERRVLAAPEGGTLHEAVADGAA
                     RPALAAADVLALAALVQRSAQALAWEGPPLDIEWALDADGRFRLLQARPVTALPRELP
                     PALAGLPAFWSNGNFRDAIPMVQTPLDWSIAAPLIEATLGTALPIAGWRPPVPQPRAR
                     LYQGRAYMAVTRMAWEFFEALGFPPARFDALTGGHQPVWQPPARVGLMTKLARGWRLF
                     RLMRALLAIRRRADVLFARSARQVARWRSHDWANADDAAIAGALQTIADEVMGSGETG
                     LRLLLTSGGGSLEQLGRWLEPCVPGRGDALAQALVAGLGAGTQGNAITSAGHAEAIEH
                     LATLAALETPVRTLLGLEGRDGRAARRDWRRKLPPNSPFALAFDDFLARWGHRAVYEV
                     SVRTPRWREDPEYLFGLIRDRVQATDAGTPMPDTAARRRALAERAGRELCAAVPAWKR
                     GLMRATAAAALQEAAQREQAKSALTAWLEPLRAALAVIGPRLVERGVLRAADDPHFLT
                     LDELRAVLDGRSDGRGLAALVADRRATHEACEALAAVDLLVAEGATLRPATDADLAAA
                     RSRPAQTASAAAATGDGTQLIGLGVAAGRAQGRARHVRHPDEGGRLAPGEVLLAPSTD
                     PAWTPLFPRAAAIVLETGGFLSHGAIVAREFGVPAVVNVAQALARIADGCPVDVDGDR
                     GRVTLSGPPAAAPEPAECPGTD"
     gene            25405..>26053
                     /locus_tag="PK144_01630"
     CDS             25405..>26053
                     /locus_tag="PK144_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64453.1"
                     /translation="MLTPRLLNAQTEREALIEHLLKLEPEDRHARFGTYVDTAAITAY
                     VDRILERNDPVLIVEDRREPWGIVAALHIAPLGDGSAELGLSVDPAWRRRGLGRTLLR
                     AGGVLAYCVGLRRVRLEIAPDNRPMRILAEQCRYQMNRSTDGELSGQRELHFDETLTQ
                     AWLWWLATLAPAAMLLLWRGAPTPREGALLGLWFGLGLYSTGTWWLYISIRVFGQA"
BASE COUNT         3634 a         9683 c         9188 g         3548 t
ORIGIN      
        1 caacttcgtc accagccacg acggcttcac gctcgccgat ctgacctgct accgcgagcg
       61 ccacaaccac gccaacggcg aggacaaccg cgacggccac ggcgacaacc attccgacaa
      121 ccacggcgtc gagggcgaca ccgcggatgg cgccctcctc gccgcccgcc ggcgcacgcg
      181 cctgaacctg ctcgcgagcc tgctgctcgc gcagggcgtg ccgatgctgc tcgccggcga
      241 cgagttcggc cagagccagg ccggcaacaa caacggctac tgccaggaca atgccctctg
      301 ctggctggac tggagccgcc tcgaaaccga agccggcttc acggccgccg tgcgccggct
      361 gacggcgctg cggcgcgaat tcgccgtgct gcgcagccgc tgctggctgc acggcgtgca
      421 tggcgacgat gcgcgccccg ctccggatga actgcgctgg ctcgggccgc acggcggcga
      481 catgagcatt ccggcctggg aagacccgca cggccgctgc ctcggtctct ggctgcacgg
      541 caccgacggc cgcgagctga tcgtgctgct caacgccggc ccggccatgg atttccggct
      601 gccggcgaac gccggcgacg gctggcaggt cgcattcgac agcagtgcct tcgaggcggc
      661 gccggggccg gccgccgtcg tgcatgccct gccggcgcgc tcgctgcgcg tgctgagccg
      721 cgtgccggcg ggcaccgcac ctgcctccag cgattccccg ccggtaccgg gccgtgcccg
      781 caagcccggc tgaccgtcat ccgcttccac gcaatcgagg gcccgtcatg gaaaaccaga
      841 ctgccacccc gcgctacgtc agccggctca cgcgcgaaac catggcgctc gtgctcgccg
      901 gcggccgcgg gacacgcctg tacgagctga cccagtggcg cgccaagccg gccgtgtact
      961 tcggcggcaa gttccggatc atcgacttcc cgctgtcgaa ctgcgtgaac tccggcatcc
     1021 agcgcatcgg cgtgctgacc cagtacaagg cgcattcgct gatccgccac ctgatcgccg
     1081 gctggggcag cgtgctgtcg cgcgagctcg gcggcggcat cgagatcctg ccggcctcgc
     1141 agcgcacctc ggacgactgg tacgccggca ccgccgatgc gatctaccag aacctcgaca
     1201 tcctgcgcgt gcgcgatccg agctacatcc tcgtgctctc gggcgatcac atctacaaga
     1261 tggactacgg cccgatgatc gccgcgcacg tcgaatccgg cgcggcgatg accgtctcgt
     1321 gcatcgagat tccgctggcg gaggcatcga gcttcggcat catcagcgtc gatgccgcga
     1381 gccgcgtcac cgccttcaac gagaagccgc aacaggccga cccgatgccg ggacgcaccg
     1441 atgtcgcgct cggctcgatg ggcaactacg tgttcaacac cgagttcctg ttcgagaagc
     1501 tgatcgagga cgccaaggac ccgaactcct cgcacgactt cggcaaggac gtgatcccgg
     1561 cgatcatccg cgaccaccac gtgcacgcct acctgttccg cgacccggcc accggccgcc
     1621 tcggctactg gcgcgatgtc ggcaccgtcg atgcgttctg ggaggcgaac atcgagcttg
     1681 ccgcgatcca gccggagctg aacctctacg accatcactg gccgatcctg acctaccagt
     1741 cgcagctgcc gccggcgaag ttcgtgttcg aggagcccgg gcgcaccggc agcgcgccga
     1801 actcgctggt ctcgggcggc tgcctgatct ccggggccac cgtgcgccat tcggtgctgt
     1861 tctcgaacgt gcgcgtcatg gatcacgcgc tggtcgagga cgcggtgatc ctgcccgatg
     1921 tcgtgatctc gccgggcgcg cgcatccgcc gcgccgtcat cgaccgcggc tgcacgattc
     1981 cggaaggcat ggtgatcggc gaggacctga cgcaggacgc gaaacagttc cgcgtcacgc
     2041 agcgcggcat cgtgctggtg accaaggaga tgctcggtca gggccgggtg ccctgagcgg
     2101 cctgcggctc ctgcgccgcg cgggccgcgt cgatcgcctg gcgaagctgc gcgaccagcc
     2161 ccggactcgc ctgcgccgcc caggcctcga cgaccggcgc gaccgcggcg cgcaggcgtt
     2221 cgagctcggc ggtcgatggc gtgttgacga gcatgccctg cgcctgcagc tgcgcgagcg
     2281 cctgcccggc ctcggcgcgg ctgtcgcgcc gctcgaagtc gcgcgccgcc accgccgcct
     2341 cgcgcagcag cgcccgctcg tccccggaca gcttgtccca ccacaggcgg ctgaccagca
     2401 ggatccacgg gctgtacacg tgccgggtca ggctcaggta cttctgcacc tcgtagaagc
     2461 gcgaggagcg gatggtgttg accgggttct cctgcccgtc gacggcgcgc gtctcaagcg
     2521 ccgtgaacag ctccgagaac ggcagcggca cggggttggc gccggtgcgg ccgaagaagt
     2581 cgaggtagac ggggttctgc atcacgcgca gcttcaggcc gtgcaggtct tcgaggcgct
     2641 cgatcggccg gcgggtgttg gtgacgctgc gaaagccgtt ctcccagtac acgagcccga
     2701 tcagcccctg cgccggcagc gtgtcgagca ggcgcgtgcc gaacggcccg tcgagcacgg
     2761 catcggcgat gcgctcgtcg tcgaacagga agggcaggtc gaagacgccg aagtcgcgca
     2821 cgatgccgac cagcgtcgcg gtcgagccga ccatcatctc ctgcgcgccg ccgatcagcg
     2881 cgttctgcat ctggatgtcg gtgccgaggc tcgcgccacc gaaggtccgc acccgcagcc
     2941 ggccgccgga cagccggccg acttcctcag cgaagaagcg ggccgcacgg ccctggttgc
     3001 tgtcctcggc cagaccgtag ccgaagcgga tcagccgcgg cttcacgtcg gccgaggccg
     3061 tgaacggcag gaccggcatg gctgcgagcc aggcgatcgc cagcagtcgc gcaatccggg
     3121 gaatgttcat gtgctgcgcc tctcgtctcc agcgggatgg ccggcgatgc atgccgccgg
     3181 ctctgtcggg aggacgaatc aggccacaga gccgatgtcg cgagtgttac ggcttgtcca
     3241 gccgtgcgcg ttcagcgcag ccactgcagc ggtgccgtga tcagccccgg aaacagcacg
     3301 aacagcccga gcagcgcgca gtaggcgccg acgtagggca gcacgccgcg cacggtctgc
     3361 agcatcggca cgccgccgac accgccgacg acgttcagca ccgtgccgac cggcggcgtg
     3421 atcaggccga tcgagccgac cagcacgaac atcacgccga agtacaccgg gtcgatgccg
     3481 gcgctgcgcg ccagcggcag cagcaccggc gcgaggatca ggatgctcgg cgtcaggtcc
     3541 atgaccatgc cgaccagcag cagcagcccg acgatcaccg ccatcagcag ccgcggatgc
     3601 gcgagcagcg gcgccagcgg cccggcgagc tgcccgggca gttcggcgag cgtgatcatg
     3661 tacgaggaca cggtcgccgc cgcgcacagg aacatcacga cgccggtcgt gcgggcgctg
     3721 cgcacgaagg ccgagtacag cgccccccgg tcgagctcgc ggtacagcac gcagccggcc
     3781 acgagcgcgt acaccgccgc gaccaccgcc gcttcggtcg gcgtgaacca gccgccgcgc
     3841 aggccgccga ggatcagcac cggcagcagc agcgcccagc cggcctccag cagcgcccgg
     3901 cgccgctcgc gcgccggcgc cggcggctgc ggaatacacc cgaagccggc gagccgccgg
     3961 cgccagacca gcaccagcac cacgcccatc agcagccccg gcacgatgcc ggcgaggaac
     4021 aggctgctga tcgaggtgct ggtgctgacg ccgtacagga tgaacggcag gctcggcggg
     4081 atgatcggcg cgatgatgcc gccggctgcg atcagcccgg ccgagccggc gagcggatag
     4141 ccgtgcgcgc gcatcatcgg cagcagcagc gtcgtgagcg ccgcggtatc ggcgatcgcc
     4201 gagcccgaca ggctcgcgag cagcatggcg gtggcgatca cgacgtagcc gaggccgccg
     4261 cgcacgtggc cgacgccggc gatcgccagc gcgacgatgc gccgcgacag gcccccggca
     4321 ttcatcagct cgccggcgag gatgaagaac ggcacggcca tcagcggata gctgtcgacg
     4381 ccggcaatca ggttctgcgc gacgagctgc gcatcgaaga cttcgagctg cagcatcagc
     4441 gcggcaccgc cgaggatcag cgcgaacgcg atcggcatgc cgagggccag cgcgccgaac
     4501 agcacggcaa ggaacagcag cagcgtcatt gcccggtccc ggtgcgtccg tgtgcgtcgc
     4561 tgtcgctgtc gctgtcgctg tcgctgtcat cggctaccgc cggctgaccc ggcacgcagg
     4621 cgcgcggatc gtgcgcgagg atccaccaca gattgcgcgc gaccagcccg gccatcgcca
     4681 gcgccgacac cagcccggac ccggcgacca gcagctgcgg aaagccgagc acggtcgagt
     4741 acgtgcgcag gccgatcacc gactgctgcc agctgccttc ggcgaacagc gccagcgccc
     4801 agagcatcag cgcatggctg atcaccgcac agagccgccg cagcggcccc ggcagccgcg
     4861 cctgcagcag gtcgaggccg aggtggcgct gctcgcgcag cgcgacgatc gcgccgagga
     4921 acacgagcca cacgaacagc agccgtgcca gttcctcggc ggccgcgaag ccggtgctga
     4981 acacgtggcg tgcgacgacg ttgccgaaca ccagcgccac catcagcgcc agcgccacga
     5041 ccatcagcgc ctcggtggcg ccaccggggc gctggctgca caggggtttc atgagtcggg
     5101 aagaatccgt ccggcagggc cgtcgcatca ggcctgcgcc gagagccggt cgacgattcc
     5161 ggcgacgatc tgcgcgggcg tggcggtgat gtcgcagacg atcgcccgct cgtccggcag
     5221 gggttcctcc agatcggcga gctggctgtc gagcagcgtg accggcatga agtgcccgtg
     5281 ccggtgcgcg aggcgctcgg cgagctgctc gcggctgccg cgcaggtaca cgaactgcag
     5341 gccgtagcct tcgcagccgc ggcgcaggcg ttcccggtag cactgcttca gcgccgagca
     5401 gctgagcacg gccccgctgc cggcgagtgc cgcggccccg agctggccgg cgagctggtc
     5461 gagccactcg acccgctcgg catcggtcag cggcacgccg gcggacatgc gcgcgatgtt
     5521 ggcgcaggta tggaaggcat cgccttcgat atggggcaga ccgagggctg cggccagacg
     5581 tccggcgatc aggctcttgc cggcgccaca gacgcccatg atcacgatgt tggtcgccat
     5641 gccggtcacg ccaccttcag aaaggcaaaa aaaacggacc gccccgctgg gggaaacggg
     5701 cggtccgcag gcgccgtcgg ggggaggggg aacggatatc acgacgcttg ccgctatgtt
     5761 agcgctatca tcccgtggcc ggtcaaggcc cggatggttg cggactgcaa aatgcttcgc
     5821 agggccacgg ggccgccctg ccggcttccg tcccgtgcac gccggcgttt cccggagaca
     5881 ccccatgccc ccccgcaagc cccatagccg cgccagcggc cgcgtcaccc tcgccgatgt
     5941 cgccgcccgc gccggggtca gcgccatttc ggtctcgcgc acgctgcagt ccccggcgca
     6001 ggtgtcggcg gcgctgcgtg cccgcatcca ggcggcgatc gccgaactcg gctacgtgcc
     6061 ggatcacgcc gcccgctcgc tggcctcggc gcgctccggc accgtcgtcg tgctcgtgcc
     6121 ctcgctcggc aacgcggtgt tcgtcgagac gctgaccgcc atccaggccg tgctgcagcc
     6181 ccgcggctgg cagatgctga tcggcaacac gcgctattca ccggccgagg aggaggccct
     6241 gctgctcgcc tggctcggtc accggcccga cggcatcctg ctcagcgggc tcgatcagac
     6301 cggacggacc cgggcgctgc tggccggcag cggcgcgccc gtcgtgcaca tgatggaaat
     6361 cgatgccacc ggcgcctgct gcagcgtcgg cagcgggcag gaggccgccg gcgcagcgat
     6421 ggccgggcat ctgctggcgc gcggctaccg gcgcatcgcc ttcatcgccg tgcagctcga
     6481 cccgcgcacc cggcagcggc gcgagggctg gcggcaggtc atgcgggatg ccggctgcta
     6541 cgagccggcg cgcgacctcg aatcgccgca gccctccagc gtcggcctcg gcgtggaaat
     6601 gctggagcgg ctgctcgcgc aggcgccgga cagcgatgcg gtgttctgct gcaacgacga
     6661 tctcgcgcac ggcgtgctgt tcgaatgcca gcgccgcggc atcgacgtgc ccggccggct
     6721 ggccgtggcc ggcttcaacg acctgccggc ctcggcgtgg acgcatccgc gcctgaccac
     6781 gatcgccatc ccgcgcaccg ggatcggcca ggaagcggcg cggctgctga ccgaactgat
     6841 ggccgggcgc acgccggcgc agcggacgat cgatctcggc ttcgagctga aggcccgcga
     6901 cagcacctga agcgccgcgc ccgctgccgg ggcacgcgct gcctcagccg gccgcggccg
     6961 gcaggtcgat gatccggccc ggctcgaagc ccgtctgcgc ggccggctcg tccggatagc
     7021 cgcgcggatt cgagacgatg cgcgtgccac cgatgcggaa gtccgtcgcc cggtggcagt
     7081 gcccgtgcac ccacagcgcg atgcgcccgt cgaacagcgc actcaggtcc gacgcaaagc
     7141 ccgtcgatac cggatcgccg gcatagaagg gctcgatgca gtccgggtgc ggcgcgtggt
     7201 gcgtgagcac gacggtcggg ccgggaaacg gccgggcgag ttcggcggcg agccagtcgc
     7261 ggtcgcgccg gtgccaggcc agcgtgtcgg ccggctccag ccggcggccg gcctgacgga
     7321 tcgcgccgcg gaaatcctgc atgaccaccg cgcaggcggc catgccggcc ccggcatcgc
     7381 cgtacagcgc gaagtcggtc cagagcgtcg tgccgaggat gcgcaggccg tcgagcagca
     7441 ggctgcgccg ctccagccag tgcagcacgc ccgcgtcgct cagcgcctgc agccgggcct
     7501 gcaccgcgcc ggtgtcggcc gggcccgcgt agtactcgtg gttgccggcg accgcgatca
     7561 gcggcacgcc cctgcagcgc ggcgccagct ccgccagccc ttcggggccg ttggcgacat
     7621 cgccggcgca gagcacggca tcgacgcctt cgagcggcag cggcggccag ggactggcct
     7681 cgcagccgcg gtggcgcgcg gcggcgcggt gcgcatcgac gtgcaggtcg ctgaggatca
     7741 gcaggcgcat ggaggcttca ggccgtcacg cgcgcgatca ccgaggcgaa cgccgcatcg
     7801 gcctgcgctt cgagcgggtg atggccttcg cgcacgcacc agcggccgcc gaccatcacg
     7861 tcgcgcacga agcggctgtc gccgccgaag atccagcgct cggtgatcgc atcgccgtgg
     7921 gcgccggcga gcagcggctg cgcgccgtcg agcacgaccc agtcggcgcg ccggcccggc
     7981 gcgagggcac cggtcggctg gccgcaggcc tgcgcgccgc cggccagcgc ccggcgcagc
     8041 aggaaaccgc cgaccgaggg ctgcccgggg ctcgcgaggc aggcgcgccg ctccagacgc
     8101 aggcgctggc cccattcgag caggcgcagc tcgccggccg gatcgacgcg cacgtggctg
     8161 tcggtgccga tgccgaagct gccgttcaaa gcgccatatt cggtggccgg gaacaggccg
     8221 tcgccgagat cggcctcggt gctcgggcag agcccggcga ccgcatggct gccggcgagg
     8281 cgcgccagct ccgtcgcatc gacgtgcgtg gcatgcacga ggcaccagcg cgcatcgacc
     8341 ggcgcgtgcg cgaacagcca gtcgagcggc cggcgcccgg accaggccag gcattcgtcg
     8401 acctcgcgcc gctgctcggc gatgtggatg tggatgacgc tggcgcgcgg cagcgtctgg
     8461 agcaccgtgc gcatgtcggc cgccgcgacc gcgcgcagcg aatgcagcgc ggtgccggtc
     8521 acgaagccct cgcgcgcgcc gtgctcgcgc acgcgttcga gcaggtgcag gaagtcatcg
     8581 acgccgatcg cgaagcgctt ctgcgcgccg gcaaccgggc gctcgccgaa gccgccgagc
     8641 cggtacagcg ccggcagcag cgtcaggccg atgccggtct cgcgcgccgc cgcgacgatg
     8701 cgcagcgcca gctccgccgg atcggcgtag gcccggcccc gggcgtcgcg gtgcaggtag
     8761 tggaactcgg cgaccgcggt ccagccggcc ttcagcagct cgatgtagac gcgccgcgcc
     8821 agcgtctgga tgtcctcggg gctcaggtgc gcgaccagcg cgtacatgcg ctcgcgccag
     8881 ctccagaagc tctcgtcgcg gcgctcggca cgctgggcca gcccggccag cgcgcgctgg
     8941 aaggcgtgcg agtgcaggtt ggcgagcccc ggcagcaccg gaccggcgag gacttcggcg
     9001 ccttcggcct gcgccggcgg acaatccggc tcgacccggc cgaggcagcc gtgcgtgtcg
     9061 acttcgagca gcacgcgccg ggcccagccc tcgggcagca gggcggcggg agcgagatag
     9121 cggttcatgg cgattctcct tgcggctggc agccggaccc ggtccggaat gcacgggcgg
     9181 gggtgcgggg gcgcgccgcg ggtgccaagc tgcctgtacc tgcatggcat agcccatcct
     9241 ggcactgccg gaggtcgccg aatgcaaacc cgtcacgatc cggaccgact gatccgcgcc
     9301 ccgcgcggcc ccgggctgcg ctgccgcaac tggcaggccg aagccgcgct gcgcatgctg
     9361 atgaacaacc tcgaccccga ggtcgccgag cgcccgtccg agctggtcgt ctacggcggc
     9421 atcggccgcg ccgcgcgcga ctggagctgt tacgaccgca tcgtcgcgac gctcgaacgc
     9481 ctcaccgacg aggaaaccct gctgatccag tccggcaagc cggtcggcgt gttccgcacc
     9541 cacgccgagg cgccgcgcgt gctgctcgcc aacagcaacc tcgtgccgca ctgggcgaac
     9601 tgggagcact tcaacgcgct cgatcgcgcc gggctgatga tgtacgggca gatgaccgcc
     9661 ggctcgtgga tctacatcgg ctcgcagggc atcgtgcagg gcacctacga gaccttcgcg
     9721 gcgatggccg atgcgcactt cggcggcgag gcgcgcgggc gctggatcct gaccgccggc
     9781 ctcggcggca tgggcggcgc gcagccgctg gcggcgacca tggccggcta cagcctgctc
     9841 gcggtcgaat gcgacccggc ccgcatcgag gcacgcctgc gcacgggcta cctcgaccgc
     9901 gccgcgggca cgctcgacga ggcgctggcg ctgctcgatg ccgcacgcgc cgccggcacg
     9961 ccggtctcgg tcggcctgcc cggcaacgcc gccgaggtgt ttccggcgct gctcgcgcgc
    10021 ggcgtgcagc ccgacatcgt caccgaccag acctcggcgc acgacccgct gcacggctat
    10081 ctgccggccg gctggacgct cgccgaatgg caggcacgcc agcaatccgg ccccgaagcc
    10141 gtcatcgccg ccgcctgcgc ctcgatggcc gtgcaggtgc gcgcgatgct cggtttccgg
    10201 gcgcgcgggg cggcggtcgt cgattacggc aacaacctgc gccagcgcgc gctcgatgcc
    10261 ggcgtcgagg acgccttcgc ctatccgggc ttcgtgccgg cctatctgcg gccgctgttc
    10321 tgccgcggca tcgggccgtt ccgctgggcg gcgctgtcgg gtgatccggc cgacatcgcg
    10381 cgcaccgacg cgctcgtgcg cgagctgatc cccggggatg cgcacctgca ccgctggctc
    10441 gatctcgccg ccgcgcgcat ccgctaccag ggcctgccgg cgcgcatctg ctgggtcgga
    10501 ctcggcgacc gccaccggct cgggctcgcg ttcaacgcgc tggtgcgcag cggcgagctg
    10561 aaggcgccga tcgtcatcgg ccgcgatcac ctcgattccg gctcggtcgc cagccccaac
    10621 cgcgagaccg aagcgatgcg cgacggctcc gatgccgtct ccgactggcc gctgctgaac
    10681 gcgctgctca actgcgcgag cggcgcgagc tgggtcagcc tgcaccacgg cggcggtgtc
    10741 ggcatgggct actcgcagca cgccggcgtc gtcatcgtct gcgacggcag cgcggccgcc
    10801 gaccgcagga tcgcgcgcgt gctgtggaac gacccggcca ccggcgtcat gcgccacgcc
    10861 gatgccggct accccgaggc catcgcctgc gcccgcgccc accgcctcga cctgccgatg
    10921 ctcggcgggg acgaagcatg agcgccctgc cctatgccga ggcgctgtgg ctcggcggcc
    10981 cgatcgcgac gctcgcgccc gccggcgacg atccctgggg gctgctgccg gacggcgcga
    11041 tcgcggtcag cgacgggcgc atcgtctggg tcggcgccgc cagcgagctg ccgggcggct
    11101 tcacggccgc cgaggtccac gacctcggcg ggcgctggct gctgccgggg ctgatcgact
    11161 gccacacgca cctggtctgg gccggctcgc gcgtcgagga atatgagcgg cgcctcgccg
    11221 gtgcgagcta cgaggacatt gcccgggccg gcggcggcat cctcgccacc gtgcaggcga
    11281 cgcgcgccgc cgatgccgga gcgctgtacg ccgccgccgc cgcccggctc gccgatctct
    11341 gcgccgacgg cgtgaccacg gtcgagatca agtccggcta cggcctcgat gtcgggaccg
    11401 agcgccggca gctcgaagtc gcgcgccggc tcggacagga acagccggtc aacgtgtaca
    11461 cgagcttcct cggcgcgcac gtcgtgccgc ccgagtacag cggtcgcgcc gggctgtgga
    11521 tcgccttcct cgccgatgag ctgctgccca tgctcgccgc cgacggcctg gtcgatgcgg
    11581 tcgatgccta ctgcgaaccg ttcgcgtaca cgccctcgca ctgcgagacc ctgttcgccg
    11641 cggcgcgcgc gctcggtctg ccggtgcgcg tgcacgccga gcagcgctcg cgctcgggcg
    11701 gcgcgcgcgt cgcggcacgc tttgcggcgc tgtcggccga tcacctcgaa tacgccgatg
    11761 aatcggacat cgccgcgctc gccgccgcag gcacggtcgc ggtgctgctg cccggagcct
    11821 tcctgacgct cggcgagcgt caggtgccgc cgctcgcggc gctgcgccgc cacggcgtgc
    11881 cgatcgcgct cgcgagcgac ctcaatcccg gcacctcgcc gctgcgctcg ccgcggctgg
    11941 ccgccgcgct cggctgccag ctctggggcc tgacgccggc cgaggcgatc gccggcctga
    12001 cccgccacgc cgcgcgcgcg ctcgggctcg ccgcgacgca cggcacgctg gaacccggca
    12061 agatcgccga cttctcgatc tggaaggtcg aacaccccgc cgaactcgcc tactggctcg
    12121 gcggacagat ctgtaccggg cgcgtgctcg ccggccgccc cgtagccgca gaggccgacc
    12181 gatgtctttc gatcccgtct acgacctgat ccccggcgat cagccgctgc tgatcagcat
    12241 cccgcacggc ggcacccgga tccccgacgc gctgcgcccg aagctcgcgc cgctcgcgca
    12301 ggcgctgccc gacaccgatc accacgtgcc gcggctgtat gcgttcgcgc cggaactcgg
    12361 catcggcgtg ctggcggcgc gctggtcgcg ctacgcggtc gacctgaacc gcccggccga
    12421 cgatgcgccg ctgtacgaat ccaacaccac gaccacggga ctgttcaccg acgaatgctt
    12481 cgacggcagc ccgctgctgc tgcccggcga acggcccgat ccgtggctgc gtgccgacat
    12541 cctcgacggc atctggcgtc cgtatcacgc ctgcctcgcg cacgagctgg cacggctgaa
    12601 ggaccgcttc ggctacgcgc tgctctggga cgcgcattcg gtgcgcagcg tgctgccgcg
    12661 cctgttcgag ggccggctgc ccgatctctg cctcggcacc aacgacggcc agagctgcga
    12721 cccggagctc accgcccggc tggtcggcat ctgcgccgcc gcgcaccgct acacgcaggt
    12781 gctcgacggc cgcttcaagg gcggctggat cacccgccac tacggccagc cgcacgcgca
    12841 cgtgcatgcc gtgcagatgg aactgtcgca ggccacgtac atggacgagg acgcgccctg
    12901 ggccttccgc gaggacctcg ccgctgacct ggtgccggtg ctgcgcacgc tgctggagac
    12961 gatgctggtc tggggacggg agcgctacgg ggcttcgtca tgaggcagcc ccgatccgtc
    13021 cgggctgcgg atgctcgaac ggcaggggca agcatccggg tgccggcgac aggccggggc
    13081 gcccgggatt cactacacag tgacgcgttt gcagcgatga ttccgaatcc ggaatccgcg
    13141 caatcatgcg accggctgat gcctgcggtc gctgcgcttc agcaggcttt cggccggaat
    13201 cgaaaagacc cgatgcagtc cttcgatcat gcgcagcgtc aacggacgct tgcgcgcaag
    13261 gatttcgtag acccggttct tgcggccgat caccggcacg aggtcgtcca ccgtcaggcc
    13321 ctgctgttcc atccggaatt tgatggcctc gaccgggtcc gggaaatcga gcggataatg
    13381 ctgccgctcg taagcctcga ccagcgtgac cagcacatcc agccgatcgc cttccggcgt
    13441 accggctccg gcgttcatca gcgtttcgat ttccgcaagc gtggcccgat aatcggattc
    13501 cgtgcggacg gggcgaattt ccatgagtgc accttgaaag ccgcggcttc agatggtctg
    13561 ggcatcgatg ccatcgtatt gcgcatgcgt gccgataaaa cggatgtaga cgacgcgatg
    13621 ggcgtaattg atccagacca cgagccggta cttgttgccg gcgatgttga acaccacccg
    13681 cccgtccctg aggatgctgg cactggcaaa ttgcgccttg acatcattcg gcgtgcgcca
    13741 gtcggccctg agggtttccc gataccaggc cagcccgggc ccgatggcat cggcatgggt
    13801 cggtgaagcc gaccagaacg ccttcagagt cgacagggca atcacgcgca tgcgttcacc
    13861 ttagtcccgc cgcgggactg ccacaagccc ggcgtgcagg tctgcccccg gtccgcgagt
    13921 tccggctgac cggccagccg acgcaatgcc tgcctgattt cccccatcca cccggaggcg
    13981 cccatgacgc ccaccccgct gcacatcctg atggtcgccg ccgagaacga cgccctgccc
    14041 ggcgccaagg tcggcggcat cggtgatgtg gtccgcgatc tgccgccggc gctggccgtg
    14101 ctcggctgca ccgtcaccgt cgtgctgccc ggttatggtt tcctcgcgca ccggcccggc
    14161 gcgcacacgc tgacgcggct gccggtcggc ttcggcggca cgcgcaccga ggtcgagctg
    14221 ttcgaggtgc cggcgcgcca tgcgcaggcc ggcgtgcgcc acatcgtcat cgagcacccg
    14281 gccttcgcgc acggcgggcg catctactgc gacgatccgc cggatcggcc gttcgcgacc
    14341 gatgccggca agtacgcgct gctgtgcgcc gcggtcgccg aagcgacggt caacggccag
    14401 ttcggccacg tcgacgtgct gcacctgcat gactggcacg ccgccttcct cacgatcctg
    14461 cgccgcttcc atccgggctt cgcgccgctt cgggcgctgc gctgcgtggt cacgctgcac
    14521 aacctcgcgc tgcagggcgt gcggccgctg cgcgggcatg cgtcctcgct ggaggcctgg
    14581 tatccgggcc tgccgtggga gccgtgggcc gtgctcgatc cgcgctggct cgactgcgtg
    14641 aacccgatgg ccgcagcgat ccgcctcgcc gatgccgtgc acgcggtgtc cccgagctat
    14701 gcgcaggaga tcctgctgcc gagcgacgtg gccggggccg gtcgctacgg cggcgagggg
    14761 ctggaggtcg acctgcagcg cgcgcgcgac gagggccgcc tctggggcat cctgaacggc
    14821 tgcgaatacc cggccccgat gccgcccccg ccggagtggc cggcgctgct gaagctcgcg
    14881 cgcggcgagg tgctgcgctg ggccggcgcc gccggcgggc cggcggtggc gagcgcgcat
    14941 ttcctcgcgc atgcgcgcct cggcgactgg ctcgaacggc cgcgcccggc aacgctgctg
    15001 accagcgtcg ggcgcatgac cgaccagaag gcggcgctgc tgctcgaacg cagcagcgac
    15061 ggtcgcagcg ccctcgatgc gatgctcgat gcgctcggtc cggacggcgc gtggatctcg
    15121 ctcggcagcg gtgatccggc ctacgagcgc gcgctggtcg ccgccagcgc ccggcatccg
    15181 aacttcgtgt tcctgcaggg ctattccgaa ccgctgtcct cggcgctgta cgccgccggt
    15241 gacctgttcg tgatgccgag ttccttcgag ccctgcggca tcagccagat gctggcgatg
    15301 cgcgccggcc agccctgcct cgtgcacgcg gtcggcggcc tgcgcgacac ggtcgaggac
    15361 ggcgtcaccg gcttcgcctt cgccggcgac ggccgcagcg cccaggccga tgcgctggtg
    15421 ctggcgctgt accgcgccct cgacgcccgc cgcgaacccg ggcgctggca ggcgatgagc
    15481 acggcggcgc gcgaggcgcg cttcctgtgg gaggacagcg cccgcgcctg tctcgtacac
    15541 ctgtaccatg ccggctgacc ggcagcggcc ggtcgtccgt cccgggaggt cctgctcatg
    15601 gaagacccct ccgccgtccc ccgcagccac gcggatcgtc cgctcgccgc cgggaccggg
    15661 aagctcggcg gcgccccggt acgcaagcgg cgcctgcggc gcggcctgct gctcgcgctg
    15721 gcgctgtatg cgctgtacgc ggccggcttc tggcactggc agcgcgcgct gctgtatccg
    15781 ggcaccgcgc gcgcggccga tcccgaggcc gcccgccgct ttccggacgc gcaggtcgag
    15841 tggatcccgc tcggcgaggc gcgcatcgag acctggtatc tgccgcccct gccgaaaccc
    15901 ggccacgatc ccgcggcccg ggcgccggca ctgatcgtcg cgcacggcaa cggcgagctg
    15961 atcgaggact ggccgcggcg cttcgagccg ttccgccggc tcggcctcgg gctgctgctg
    16021 gtcgaatacc ccggctacgg ccgctccggc ggcacgccgg acgaggccgg catccgccgg
    16081 ctgttcgccg cggcctggga ccggctcgcc gcgcgcccgg acatcgaccc ggcgcgcatc
    16141 ggcggactcg gcatctcgct cggcggcggc gcgatcggca cgctgatccc gcagcgaccg
    16201 ctggcggcgg tgatcctgct gtcgaccttc accgacatcg gcgccttcgc cggccgctac
    16261 ggcctgccgc gcgatctggt gctcgacccc tacgacaccc gcgccgcgct cgccggctac
    16321 gacgcgccgg tgctggtgat ccacggccgc cgggatggcg tgatcccgta cgcacacgcg
    16381 caggcgctcg cggcggccgg ccgtcacgcg acgctgcacc tgcatgactg cgcgcacgtg
    16441 tgctgggaac ccgaacgcct gccgctgtgg gcggagcttg aagccttcct gcggcgtacc
    16501 ccgttgctgc cagccagtgt tcaccaggat tgacgcctgc agggattccg ccgtatgtcc
    16561 ctgtccgcac acgcgggcgg atcggcctgc aactgattca ggtcagcctg ccgggcaggg
    16621 ccgcaacggt aatgtaaagc gttcaccggc cggccctgcc tgcccccgcg gctccccgcg
    16681 ccgcggaggc tgcaactgcg tcgcgcagac ggcgcagcgg cggcttgccc ggtcttgtcc
    16741 cccagaaccg gccccgatgc cggcccgcgc ccccaaggag agacaccgac atgccctcgc
    16801 tcgcgacgca gtacctcggc ctgaagctgg ccaatccgct gatcctcggc gcctcgccgc
    16861 tcggccatga acccctgctc gcgcagcgcg tcgccgatgc cggcatcggc gcgatcgtca
    16921 tgcattcgct gttcgaggag gacctgctcg aagacgtgac ccagcgccag ctgctcggcc
    16981 tgccgaccga cccggacagc cagcgcgcgc tgttccgcgg ctcgctcgat cgttacctga
    17041 agcagctcga aaagctgaag aagagcgtcg gcgtgccggt catcgccagc ctcaacggcg
    17101 tctcggccag cggctggatg gagcaggcga aagcgctggt cgatgccggc gccgatgcgc
    17161 tggagctgaa cgtctattac ctcgccgcct cgccggccga atcgtcgacg gcggtcgagg
    17221 cgcgctacat cgagctggtg cgccagctgc gcgcgacgct gcggattccg ttcgcggtca
    17281 agctgtcctc gcagttcacg gcgctgccgc acttcctgcg ccggctcgaa gccgagggcg
    17341 ccagcggctt cgtgctgttc aaccgcttct accagccgga catcgacctc aacggcgtgc
    17401 gcctgatcca ccgcccgatc cgctcgcgct ccgaggacgt gctgatggcg ctgcactgga
    17461 tcgcgatgct gcgcgggcgc atcaacggcg gtctgtcggc cagcggcggc gtgcagcacg
    17521 ccgatgacgt gctgaaactc ctgctcgccg gcgccgacac cgtgcagatc gtcggcgcgg
    17581 tgctcaacga aggtccgggc ctggtctccg acatcctcgc cggcgtcggc gaatggctgg
    17641 acgcgcacgg ctacgactcg gtcgccgacc tgcgcggacg cttcagcgag aaccgcatct
    17701 ccgaccccgg cgcctacgag cgcatccact accgcggcgt gctcgacaac tggcgcgcgc
    17761 cgggcgaagc ctggcgctga acgacgcggc cgggcggcac cgccgcccgg catgccgtca
    17821 ggccgccagc tccggcgcag ccgcctgcgg ctgcgcgccg ccctgaccga gccgggcgcg
    17881 cacggcgacc agctcggcga ggatggacac ggcgatctcg gccggcgtgc ggctgccgat
    17941 cgccagcccg accggcgcgt gcagccgctc gatcgccgcg gcttcgacgc cgacctcggc
    18001 gagccgcgcg cggcgcgcct gcgaagtccg ctgcgagccg agcgcaccga cgtagaaggc
    18061 cggcgaggcg agcgcttcca tcagcgccag atcatcgagc cgcgggtcgt gcgcgagcgt
    18121 gaggatcgcg ctgtgcgcat ccggcgcgaa ggcgagcacg gcgtcatccg gcgaaccgcc
    18181 gaggcgcgtg acaccttcgg cgagcggctg tccggcatga ccggggcgcg gatcgcagac
    18241 cgcgaccgcg tagccgagcg cctgggccat cggcgcgagc agcgcggcga cgtcggtcgc
    18301 gccgatcagc agcagccgcc agcgcggtcc gaagggctgg cgcagcagcg ggccgtcgcc
    18361 gctgaaggcc gtcgtcgcat cggccggcac cagccgctgc gcaccgctcg cgagcttcag
    18421 ctcgcgcagc agatgcccgc cggcggcagt cgtggccagc accgcgtcga tccaggccgg
    18481 atcggtcagc gtctccagca gcaggtgcag cgtgccgccg cagggcagct gcaggcgcac
    18541 ggcctcctcg cgcgtcgcgc cgaacacgat gcgctgcggc agcgccggtg gtccggtgcg
    18601 cgccagccgc gcgaccagtt cctcctcgac gcagccgccg gacaccgagc cggcgatcgc
    18661 gccgtcgcgc acgcggatcg ccgcgatcgc cccgggcggg cgcggagccg agccgtaggt
    18721 ggcaagcacg gtcgcgagcg cgaccgcctc gccgctgcgc cgccagccgg cggcgcttgc
    18781 gagcacatcc agatcgagcg actgcatgct cacgcgctcc gtcagctctt ggcgaccggc
    18841 atcagcggca gccggcggtg gcgttcgccg gtcagcgcga acagcgcgtt gccgacggct
    18901 gcggcgatcg gcggcgtgcc cggctcgccg acgccggtcg gcggcgcctc gctcggcacg
    18961 atgtgcacct cgatctgcgg ggactcggac aggcgcacgg ccgggtagtc gtcgaagttg
    19021 ccctgctcga cgcgcccgtc cttcagcgtg atctgcccgt acagcgccgc gctcaggccg
    19081 tagacgatgc cgctctcgat ctgcgcgcgc accatgtccg gattgaccgc cgtgccgcag
    19141 tcgacggccg cgaccacgcg cgtcacctgc acccggccgt ccttctgcgt gacctcggcg
    19201 atctcgcaga ccacgctctc gaaggattcg tggatggcga tgccacggcc gctgcccttt
    19261 tccggcggcc ggctccagcc ggccttggcg gcggcgaggt tcaggcactt caggaagcgc
    19321 ggatcctggt gctttgcgag cagcctgcgc cggtattcga gcgggtcgat gccggcggcg
    19381 tgtgcgcatt cgtcgacgaa gctctcgacg acgaagccgg tatgcgaatg cccgaccgag
    19441 cgccaccact gcaccggcac cggcgacttc gggctgtgca ggtcgacctg caggttcgcg
    19501 accgcatacg gcaggtccga ggcaccctcg accgaggtcg catcgatgcc gccgcgcacc
    19561 atgaaggcct cgaacggcgt gccggcgagg atgctctggc cgacgatggt gtgcagccac
    19621 gccaccggca gaccatcggc tccgagcccg gcgcgcagcc gcgagaccca gtgcggccgg
    19681 taccagccgc cgcgcgtgtc gtcctcgcgc gaccagacgg tcatgaccgg cttgccgggc
    19741 atggccttcg cgacctcgac cgcctcgttg ctccagtccg aggccggatt cgcgcgccgg
    19801 ccgaagccgc cgccgagcag cgtcgtgtgg atcgtcacct gctcgggctt cacgccggcg
    19861 atcttcgcgg cattgccctg atcgacggtc tggaactgcg tgccggccca gatctcgacg
    19921 gcatcggcat cgacgcgcac cgtgcagttc agcggttcca tgcaggcgtg cgcgaggtaa
    19981 ggcacctcgt attcggcatc gagcgtcttc gcggccgttt tcaatgcacc gggggcatca
    20041 ccctgggcga cggcgaccgt gccgggctcg cgcgcgagct tctggaatcc ggcccgctgc
    20101 gcggccgtgg aaaagccggc gttcggacca tcgtcccact gcaccttcag cgcctcgcgg
    20161 cccttcagcg ccgcccaggt gttgctggca tagacggcga caccgccggc gacggccttg
    20221 accgcgagca cgccgggcac ggcctgcgcg gccgccgagt cccactgctt cggcttgccg
    20281 ccgaacaccg gcggccgcgc gagcaccgca aacagcatgc ccggcacgcg cacgtcgatg
    20341 ccgaactgcc cctcgccgct gaccttgtcc ggaccttcga ggcggggcgt ggccttgccg
    20401 atcagcttga agtccttcgg attcttcagc gtcacgctcg ccggcggcgt gcgtctgccg
    20461 gcttcttcgg ccagttcgcc gaacgacagc ttgcggtcac cgtgcagcac gaagccgttc
    20521 tcggcccggc agtcgacggc cggcacgttc cagcgcgccg cggcggtctc gaccagcatc
    20581 gtgcgcgcga cggcgccgat gcggcgcatc tgctcccagc tcgatgcgac gctggtgctg
    20641 ccgccggtca tctggatgcc gaacaccgga tgcgcgtaga cgatcgccgc cggcgccggt
    20701 tcgacgcgca cctggctcca gtcggcttcg agctcctcgc agatcagcat cggcagcgag
    20761 gtgtagacgc cctgccccat ctcggccttg ttgcagagga tggtcgtcgt gccatccggc
    20821 gcgatgcgga tgaaggcatc cggcgggtat tccttcgccg gcgcatcggc ggcctgcgcc
    20881 gcctgcaggc gcttcagcgt cgccggcaga tgaaaggcca gcaccaggcc ggcgccgagc
    20941 atcgcgccgc ccttcaggaa accgcggcgg ctgatcgaga catcgaggct catggttcag
    21001 gcactccgct cggcgccggc ggcgcggtgg atggcggcgc ggatgcgccc ataggtgccg
    21061 cagcggcaca ggttgccgcc catcgcgttg tcgatgtcgg catcgctcgg cttcggattc
    21121 gaggcgagca gtgccgcagc gctcatgatc tggccggact ggcagtagcc gcactgcacg
    21181 acgccttcgt cgagccaggc ctgctgcacc gggtgcaggc cgccggccgg ggcgaggccc
    21241 tcgatcgtcg tcagcttgcg accggccgcc gccgacagcg gcgtcaggca ggcgcgcacc
    21301 ggtgcgccgt cgaggtgcac ggtgcaggcg ccgcacaggc cgcgaccgca gccgaacttg
    21361 gtgccggtca gcccgagcac atcgcgcagc gcccacagca gcggcatgtc gccgggcgca
    21421 tcgatctcac ggctctggcc gttgacttcc acggtggtca tcgggcgttc ctcggaaggg
    21481 ggggtggtga cggaaatgcg ggggaaatgc cgacacgacg ggcagaccat agacgccggc
    21541 ttgcatgcag ctactgacgc gtgatgaatg gcagcttgac ggctttatct gaacgacgtt
    21601 caattttgaa catcgttcag atacgaacgc cgttccggtc cctgccgatg aacccctgcc
    21661 cgcccgccga ccgccgcagc cgccggcgcg aacgcaccgc cgaagacatc ctcgccgccg
    21721 ccgtcgcgct gttcgccgaa cagggctggg cagcgacgac gatggagcag atcgcggcgc
    21781 gcgccgacat cgccaaggcg acgctgtaca gccactttcc ggtcaaggag gcgatcctgg
    21841 tcgcgcagat gcagcaggag ctgcgcatgc agtggccggc cttcgagcag cgcatccacg
    21901 agaccaccgg cacgcgcgcg cagctgctgg cgctgttcga gctggccgcg ccctggctcg
    21961 aagcgcatcg cgacgtgatg gcgccgtacg tcgcctaccg gctgtcgcgc ccgcgcggcg
    22021 acggcagcca gcgcagcggc atggacaagc tgttcacggc catcgtcgag cgcggtcgcg
    22081 cgcgcggcga attgcgcgac gacctgccgg tggcggtgct ggtcatgcac ctgcagtttg
    22141 cgtacctcgc cgagcttctg cgctggctcg aagccccgga agccggccac gaactcggcg
    22201 cggcgctcgc ggcgatggcc ggcctgttcc tcgatggcgc cgcgcagcgc ccggagcagc
    22261 cgtcatgagt gcgtccgacc gcacggaaac ccgcctggaa gccgccccgc ggatcacgct
    22321 gctcgacccg gccgctgccc gggcgcaggg catggacagc gccggcggca agggctggca
    22381 gctcgcacgc atggcgcact acgcgctgcc ggtgccggac ttccgcgtgc tgccggccgc
    22441 ggcggcgctg gcggcggtcg aggcgaccgg cctcgatgcc gatgccgccg acgccggcga
    22501 cgatccggca gcgctggccg ccgtggccgc ggcgctgagc agcggcgagc cgccggcctg
    22561 gctgcaggcg gtggtcgacg ccgtgctcgc gctgcccggc ttcgaccggc cgctggccgt
    22621 gcgctcgtcg gcgcgctgcg aggattcggc caccgcctcc ttcgccggca tccatgactc
    22681 gaagctcaac gtgccgccgg acgcgccggc cgtctgcgcg gccgtgcgcg cggtctgggc
    22741 ctcggcctgg agtgcgcatg cgcaggccta ccgccggcgg ctcggcctcg actggcgcgc
    22801 cgcggcgctc gccgtggtcg tgatgccgct ggtgccggcg cgcgccgccg gcgtggcgtt
    22861 ctcctgcgat ccgcagaccg gcgcgaccga tgtcatcgtc atcgaagccg tgaccggtct
    22921 cggcgaagcg ctggtcggcg gtcaggtcga gccggaccgc tggcggctgc gcctgcccgc
    22981 gcccggccac gccgcgctga tcgccgacac gcgcatcggc cgcaaggaac ggcgcgtgct
    23041 cgccgcaccc gaaggcggca ccctgcacga agcggtcgcc gacggtgccg cccggccggc
    23101 actcgcggcc gcggacgtgc tcgcactcgc cgccctcgtg cagcgcagcg cccaggcgct
    23161 cgcctgggaa gggccgccgc tcgacatcga atgggcgctc gatgccgacg gccgcttccg
    23221 gctgctgcag gcacggccgg tcacggcgct gccgcgcgag ctgccgccgg ccctggccgg
    23281 cctgccggcg ttctggtcga acggcaactt ccgcgacgcg atcccgatgg tgcagacgcc
    23341 gctcgactgg agcatcgccg cgccgctgat cgaggccacg ctcggcaccg cgctgccgat
    23401 cgccggctgg cgcccgccgg tgccgcagcc gcgcgcgcgc ctgtaccagg gccgggcgta
    23461 catggccgtg acccgcatgg cctgggaatt ctttgaggcc ctcggctttc cgccggcgcg
    23521 cttcgacgcg ctgaccggcg gccatcagcc ggtctggcag ccgccggcgc gggtcgggtt
    23581 gatgacgaaa ctcgcgcgcg gctggcgcct gttccggctg atgcgcgcac tgctcgcgat
    23641 ccgccggcgt gccgatgtgc tgttcgcgcg cagcgcccgc caggtcgcgc gctggcgcag
    23701 ccacgactgg gcgaacgccg acgatgccgc gatcgcaggc gcgctgcaga cgatcgccga
    23761 cgaggtgatg ggcagcggcg aaaccggcct gcgcctgctg ctgacctccg gcggcggctc
    23821 gctcgaacag ctcggccgct ggctggagcc ctgcgtgccc ggtcgcgggg acgcgctggc
    23881 gcaggcgctg gtcgccggcc tcggtgccgg cacgcagggc aatgccatca ccagcgccgg
    23941 tcatgccgag gcgatcgaac acctcgcgac gctcgccgcg ctggaaaccc cggtgcgcac
    24001 gctgctcggg ctcgaaggca gggacggccg ggcggcgcgc cgcgactggc ggcgcaagct
    24061 gccgccgaac tcgccgttcg cgctcgcctt cgatgacttc ctggcgcgct gggggcatcg
    24121 cgcggtctac gaggtctcgg tgcgcacgcc gcgctggcgc gaggacccgg agtacctgtt
    24181 cggcctgatc cgcgaccgcg tgcaggccac cgatgccggc acgccgatgc cggataccgc
    24241 cgcccgccgc cgggcgctgg ccgaacgcgc cgggcgcgag ctgtgtgccg ccgtgccggc
    24301 ctggaagcgc gggctgatgc gcgcgaccgc cgccgccgcg ctgcaggagg ccgcgcagcg
    24361 cgagcaggcg aaatcggcgc tcaccgcctg gctggaaccg ctgcgcgcgg ctcttgcggt
    24421 catcgggccg cggctggtcg aacgcggcgt gctgcgcgcc gcggacgacc cgcacttcct
    24481 gacgctcgat gaactgcgcg ccgtgctcga cgggcgcagc gacggtcgcg gtctcgcagc
    24541 cctggtcgcc gaccggcgcg ccacgcacga agcctgcgaa gcgctggcgg ccgtcgacct
    24601 gctggtcgcc gagggtgcca cgctgcgccc ggccaccgac gccgatctcg ccgccgcacg
    24661 cagccggccg gcgcagaccg catcggcagc ggctgcgacg ggcgacggca cgcagctcat
    24721 cggcctgggg gtcgcggccg gccgtgcgca gggtcgggcg cggcacgtgc gtcatccgga
    24781 cgaaggcggc cggctcgcac cgggcgaggt gctgctcgcg ccatcgaccg atccggcctg
    24841 gacgccgctg ttcccgcgcg ccgcggcgat cgtgctcgaa accggcggct tcctgtcgca
    24901 cggcgcgatc gtcgcacgcg agttcggcgt gccggcggtc gtcaacgtcg cgcaggcgct
    24961 cgcgcgcatc gccgatggct gcccggtcga tgtcgatggc gaccgcgggc gcgtgacgct
    25021 gtccggaccg ccggcagcgg cgcccgaacc ggccgagtgc ccgggcacgg actgaagaca
    25081 ggcgggcccg cccggccctt gcggcccggc ccgcccatcc cgttcgccgg ccggaagttt
    25141 catccgcgcc ccggatttgc tcggcaaccg tcccgggccg cgccggaacg ccggcccgga
    25201 gcgcgggtct cgtgagcgga ccgggcccgg ccgcgtcctg cggccgcagt gcgtccgctc
    25261 gccggcggcg caggactttc cttcaagagg atgaatcggc acgcattgtt gcaaagcaac
    25321 aaatgcagtg gcagacgtga ttcgcatgtg ctgcactgca gcagtagtaa tccgctattc
    25381 cggacaaccc ggaaggagtg catcatgctg accccccgac tgctgaatgc gcagaccgaa
    25441 cgtgaggccc tgatcgagca cctgctgaag ctcgaacccg aggaccggca cgcccgcttc
    25501 ggcacctacg tcgacacggc ggccatcacc gcctacgtcg atcgcatcct ggaacgcaac
    25561 gacccggtac tgatcgtcga ggaccggcgc gagccctggg gcatcgtcgc cgcgctgcac
    25621 atcgcaccgc tcggcgacgg ctcggccgag ctgggcctga gcgtcgatcc ggcctggcgc
    25681 cggcgcgggc tcggccgcac gctgctgcgc gccggcggcg tactggcgta ctgcgtgggc
    25741 ctgcgccggg tacgactgga aatcgccccg gacaaccgcc cgatgcggat actggccgaa
    25801 cagtgccgct accagatgaa ccgttcgacc gacggcgaac tgagcggcca gcgcgagctg
    25861 cacttcgacg agacgctgac ccaggcctgg ctgtggtggc tggcgacgct ggcgcccgct
    25921 gcaatgctgc tgttgtggcg cggggcgccc acaccgcgcg aaggcgcgct gctgggcctg
    25981 tggttcggac tcggcctcta ctcaaccggc acctggtggc tgtacatctc catccgcgtc
    26041 ttcggccagg cac
//
LOCUS       DAOQIE010000013        25961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_827_length_25961_cov_7.550181, whole genome shotgun sequence.
ACCESSION   DAOQIE010000013 DAOQIE010000000
VERSION     DAOQIE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 25961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25961
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_25961_cov_7.550181"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK144_01635"
     CDS             complement(<1..707)
                     /locus_tag="PK144_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF64454.1"
                     /translation="MATTDYKIIEHTYDVVVVGAGGAGLRATFGMAQKGLRTACLTKV
                     FPTRSHTVAAQGGISAALGNMGPDSWQWHMYDTVKGSDWLGDQDAIEYMCREAIPAII
                     ELEHQGVPFSRTEEGKIYQRPFGGMTTNFGEGQAQRTCAAADRTGHAILHTLYQQSLR
                     HNAEFFIEYFALDLLMEDGECRGVLAWDLAEGTLHVFRGQQTVLATGGYGRAYFSATS
                     AHPCTGDGNGMVLRAGLP"
     gene            complement(716..1105)
                     /gene="sdhD"
                     /locus_tag="PK144_01640"
     CDS             complement(716..1105)
                     /gene="sdhD"
                     /locus_tag="PK144_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNF64455.1"
                     /translation="MSKSSLRTPLAHVRGLGSSRSGVHHWWLQRLSAVILVPLVLWFA
                     VSLLSVAHADHATMKAWVSRPFNTALLVVLLTAMFYHARLGLQVVIEDYVHEELVKLG
                     ALLAMNLVVIVAGATAVISVLKIAFGG"
     gene            complement(1119..1496)
                     /gene="sdhC"
                     /locus_tag="PK144_01645"
     CDS             complement(1119..1496)
                     /gene="sdhC"
                     /locus_tag="PK144_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNF64456.1"
                     /translation="MATDKRPLSPHLQVYKPQMTSVLSILHRGTGVALSIGTLLIVYW
                     LAAAAVGPEAFERAQHVLGSMLGQLFLFCWTWALFYHLCNGIRHLLWDAGHGFDIPTV
                     YKTGKTVLGAATALTFVCWIIVT"
     gene            complement(1791..1979)
                     /locus_tag="PK144_01650"
     CDS             complement(1791..1979)
                     /locus_tag="PK144_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HNF64457.1"
                     /translation="MSVTCDEPSPAVEPVPPAAPAGHPDSLDETPPPATTTEYGGRAG
                     PEPTRYGDWEKNGRCIDF"
     gene            2192..3154
                     /locus_tag="PK144_01655"
     CDS             2192..3154
                     /locus_tag="PK144_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNF64458.1"
                     /translation="MTDDTDPTADTCLHPLPAPGLIRVAGAEASAFLQGQLTQDVRRI
                     DATTTLRAACCNPKGRVLATLRLFARDQALWLWLPAERAAALAQRLKMYVLRAKVELS
                     DVSPQFAALGVSGAGAGARLAGVLGVPVPEQADRALTHDGLTVLGLPAAPPRLAVIGD
                     PARIDALQQALLAAGARAATAEDWAALDIAAGIAEVHGPLVEAFVPQMLNLDRTDGIS
                     FSKGCYTGQEIVARVHYLGQIKQRLYRAHVDAPAAPAVAAALIADQAGPNEQAGQIVS
                     VAADPAGGWQMLAVVTTQLAARDAVRLADGTPLRLQPLHAGQDT"
     gene            3206..3442
                     /locus_tag="PK144_01660"
     CDS             3206..3442
                     /locus_tag="PK144_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64459.1"
                     /translation="MEVMEASRTSLRPHIAEILRTDDYGPAPAAWRHAHSPPLRKPEI
                     RLIPQALICRRDQTFDGIIINLQNIKLVIPPPPR"
     gene            complement(3759..4958)
                     /locus_tag="PK144_01665"
     CDS             complement(3759..4958)
                     /locus_tag="PK144_01665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNF64460.1"
                     /translation="MSSSSARSDDIVVCAPVRTAIGRFGGSLVDVPAAALGAHVIAAS
                     LRRAGLDPAQVDSVVFGNVIQAGNGMNLARQAAIGGGLPVGVPALTVNRVCGSGAQAL
                     ASAAAEILAGQAEVVIAGGTENMDRAPYLLPAMRHGARMGNAQAIDAMLRDGLNDAFS
                     GEHSGWHTEDLVAKFGLSRADQDAFAARSQQRFAAAQAAGHFVTEIEPLEIRTKKGPA
                     LFAADEGNRPETTLESLAKLRPAFRDGGTITAGNAPGVNAAAAAMVVCKRAWADAHGL
                     TPMLVLRASAVVGVEPGLFGYGPVPAIRKVLTRTGWSVGEIDRVEVNEAFAAVALVAQ
                     QQLDIDPARFNVDGGAIAHGHPIGATGAVLIVRLAHALARTGGQRGIVSLCIGGGQGI
                     ALALERP"
     gene            5472..6626
                     /gene="nifV"
                     /locus_tag="PK144_01670"
     CDS             5472..6626
                     /gene="nifV"
                     /locus_tag="PK144_01670"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HNF64461.1"
                     /translation="MDRSILIHDTTLRDGEQTAGVAFNRAEKLAIAAALDLAGVQELE
                     IGIPAMGAEEQDDMRAIVGLGLQTTLVAWCRMSRKDLDAALATGVRAVNLSTPVSDTQ
                     IQLKLGRDRRWVLANVAQMVAMARDAGFDDVRVGGEDASRADPDFVAEIAATAAAAGA
                     RRFRYADTVGLLEPFGTFERITRLRAATAIELEIHAHDDLGLATANSLAAVRAGADFV
                     STTVNGLGERAGNAPLEEVATALSVLHGRDCGVRLAQLPAISQLVAEASGRPVPVGKS
                     VVGDAVFTHESGIHVDGLLKDPRTYQGLDPARLGRHHRLVLGKHSGTASVRRAYAELG
                     LRLAEDEVQRLLGRIRVHAGSTKRAPTADELLGFWHETRAPRPYVAGTLN"
     gene            6687..7454
                     /gene="cysE"
                     /locus_tag="PK144_01675"
     CDS             6687..7454
                     /gene="cysE"
                     /locus_tag="PK144_01675"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNF64462.1"
                     /translation="MTQTVRPGLLARLREDVLAVRARDPAARSAFEVLATYPGLHALI
                     LHRIANGLWRRQWRFTARLLAGLARGLTQIDIHPAATIGRRMFIDHGCGVVIGETAEI
                     GDDVTLYQGVTLGGTSLSRGKRHPTLGDGVTVGAGAKVLGAIEIGAGARVGANSVVIR
                     DVPAAATVVGIPGRVVPRGDTARADGIDLDHHLMPDPVGRALACLIQRIDDLEARLAA
                     AGPAIPGLDHPHACTDCGDACDQARHAAAAHPAALHS"
     gene            7466..7798
                     /locus_tag="PK144_01680"
     CDS             7466..7798
                     /locus_tag="PK144_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNF64463.1"
                     /translation="MSTLSDRLQTLSAADEFFELFKIDVDPHVVHVNRLHILKRFHDY
                     LKRSPVQADLSEADEFAAYRERLVQAYLDFTVSDAVTEKVFKVFRQAQGQGFVGLDQI
                     ERIERPAT"
     gene            7848..8693
                     /locus_tag="PK144_01685"
     CDS             7848..8693
                     /locus_tag="PK144_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNF64464.1"
                     /translation="MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIINPYDLFSLEE
                     ALRLKDQFGGKVTVITMGPPQAEDALRKALSFGADEAILLTDRAFAGADTLATSCALA
                     QALRQIGEKEPVDLVFTGKQTIDGDTAQVGPGVAKRLDLQLLTYVAKIDELDLAGRTI
                     TVQRRAEGGVQVLKTRLPALITMLEGTNEMRFATMTDMFRAARSPLTVWNKDDAGITD
                     ISKIGLKGSPTVVSKVFAPKPKSVPAQMLDIGGKSQTEIADLIIDTLSAKHAPLYGEL
                     FDRAL"
     gene            8721..9815
                     /locus_tag="PK144_01690"
     CDS             8721..9815
                     /locus_tag="PK144_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNF64465.1"
                     /translation="MSEAPKPAAPAARGRKKLELDPRLAAYKGVWVFIEHERGTVHPV
                     SWELLGEGRKLADKLGVELAGVLLGGPGDPLDAFAATAHTYGADLVYITRSPVLAGYR
                     NEPYTKALTAVVNQFQPEILLLGATVQGRDLAGSVATTLATGLTADCTELAIDPDNRS
                     LAATRPTFGGSLLCTIQTLNYRPQMATVRPRVMSMPAQQPERSGRVLEAPVELVEDDI
                     IAKLLSYIPDDATDQAQLAYADVIVAGGRGLGKPENFKLLWDLAEVLGAEVGGSRPVI
                     QNGILSADRQVGQTGKTVRPKLYIAAGISGAIQHRVGMEASDVIIAINTDPKAPIFDF
                     ATYGIVGDAVQILPALTAACRARLAMKRAG"
     gene            9827..11122
                     /locus_tag="PK144_01695"
     CDS             9827..11122
                     /locus_tag="PK144_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNF64466.1"
                     /translation="MEKFDVIVVGAGAAGNAATYTLAKAGLNVLQIERGETSGTKNVQ
                     GAILYADALERIIPDFRDDAPLERHIVEQRIWIMEDKSHVGTHYRSDSFNEERPNRYT
                     ILRAQFDKWFSGKVRESGALVINETTVLELLKNDAGRIVGVRTDRVGGDIYADAVILA
                     DGVNSLLARKAGLREEIEAKNVALAVKEVHFLPQDVLESRFNIQEEEGVVIEIAGTIT
                     RGMAGTAFLYTNRESVAIGIGCMLEDFKGSGVSPYTLLEDLKNHPSIKSLIAGGEVKE
                     YAAHLIPEGGYKALPKLAGDGWLMVGDSAGFVNAVHREGSNLAMTSGRLAAETLIALK
                     TAGKPATAANLQAYVEALNDSFVMKDLRKYQHVPALLEEKQFLTTYPTLLNRAAETML
                     RVDGVDKKTKQRSALGAFVQDRSLFGLMKDTYKLWRAFR"
     gene            11119..11406
                     /locus_tag="PK144_01700"
     CDS             11119..11406
                     /locus_tag="PK144_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNF64467.1"
                     /translation="MSKVEEKLFQNRYRVDEGRPHIQIIDQEKCTECGTQDCTYLCPA
                     ACYAKAEDGRHVVLTTDGCLECGTCRVICSEFRNVAWEYPRGGYGILFKFG"
     gene            11417..11971
                     /locus_tag="PK144_01705"
     CDS             11417..11971
                     /locus_tag="PK144_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64468.1"
                     /translation="MLTPSLPLRAVPLAEYPFGLGVTDGAGFRALREAAADDAASVAG
                     AYLEQAAAGVEPARREFLLWSAHALAPNHLPVYFALYKFLFHQKRLVDAERVALMALG
                     ESARQGGFPVRWNELRHWTADWSDGPGRFYLFSLKALAFIRLRLHGLKAAGPLLEKLH
                     ELDPQDQVGGSVITDLARACSAAG"
     gene            12087..12545
                     /locus_tag="PK144_01710"
     CDS             12087..12545
                     /locus_tag="PK144_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNF64469.1"
                     /translation="MDCINRFLAHAIALEGSAAGRYEDLANSMSALGDAEVAAFFRQM
                     AEFARMHQAEAIDRAGFRDVEQPPEDGYEWGPGESPEDAAWAGVDSFLDVTAALELAL
                     EAERSSEAFYRRVARETSDAEVRGFAESFAEEEVGHVNALLARLERRASV"
     gene            12661..13188
                     /locus_tag="PK144_01715"
     CDS             12661..13188
                     /locus_tag="PK144_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64470.1"
                     /translation="MAGSGDGREPRSRRGKNGCHPLKKIHLNQQPDEINASFSPAGIT
                     PAHTGSSQPGRPKAPAADAASPPPSHPPALQSEPEMSTMNLNIRTLIATALISFAAAG
                     SVNALASEADSLPMPSWNPEQTVYGSPIGLSQAPAAMTRFEARMSATGSGHELCAARV
                     ALNPNAGRNASLQAC"
     gene            13297..13611
                     /locus_tag="PK144_01720"
     CDS             13297..13611
                     /locus_tag="PK144_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64471.1"
                     /translation="MRGFAPSLARAVLPAAFALASAIGSIALAAGPAAVPSRDAALLA
                     PQLPPALRDTDPALPTLTSPDAAPARRPAADNGRVGDDTTFCNVFLNESERERSGATG
                     FC"
     gene            13740..14009
                     /locus_tag="PK144_01725"
     CDS             13740..14009
                     /locus_tag="PK144_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64472.1"
                     /translation="MNLKTAIAASLLTLSVATIAHAGERDSYPQPSWDPDTSDHLHRP
                     AAPLGAANASIDERMRATGSSTDFCITELYPLNPRGGNNGHAGIC"
     gene            14112..14396
                     /locus_tag="PK144_01730"
     CDS             14112..14396
                     /locus_tag="PK144_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64473.1"
                     /translation="MNVKTAIAASLLTLSIAGAAHAMDRYDTGSRPGAYPEYPSETGN
                     GMHQRAPVTQSGSPGFDQRMRATGSGFDYCQQALYPLEPSSGRNVLGGTC"
     gene            14472..14741
                     /locus_tag="PK144_01735"
     CDS             14472..14741
                     /locus_tag="PK144_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64474.1"
                     /translation="MNLKTAIAASLLTLAAATAAHAGDSDAYPRPDWNPNASDYLYKQ
                     PNPYGMSTAASIDERMRATGSGTEFCVEPYALNPRDGSNSHAGVC"
     gene            complement(14973..16781)
                     /gene="phaC"
                     /locus_tag="PK144_01740"
     CDS             complement(14973..16781)
                     /gene="phaC"
                     /locus_tag="PK144_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNF64475.1"
                     /translation="MGVAEKFEIQVPDPLAITSTLHDIVASGDRLIGSLFRERIRKPV
                     RVPEWLGDELGIGRAFGEATLRLWRDPLKLLDRQTALWVDSLDLWARTCSALSGTRVS
                     PIIQPERGDRRFKDEAWTEFAVFDHIKQSYLLSARWLLETMKGVEDIDEQHARKVEFY
                     TRQLIDALAPTNFALTNPEALREIFRTGGESLLRGLRNLMRDIERGKGRLSIRMTDLD
                     AFTLGDNIATTPGSVVFQNDMMQLIQYAPLGDTVRRRPLLIVPPWINKFYILDLRDKN
                     SFIRWAVAQGNTVFVISWVNPDASYAARGFDDYLEHGITAALQAIERATGEREVNAVG
                     YCLGGTLLSASTAWYAAGDHQPIASTTLFTTMLDFARPGELEVFIDDQQLDAIERRMQ
                     KQGYLDGAEMASTFNMLRANDLIWSFAVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHS
                     FYLRNMYQHNRLREPGGITLCGRPIDLRQVRTPVYFLSAVEDHIAPWKSTYAGTRLFG
                     GPVRFVLGGSGHIAGVINPPAAKKYGYLTSPRLELDADEWLATATQTAGSWWPDWDAW
                     LDQQDATRVPARHAGDGALAVLEPAPGSYVKRRLAR"
     gene            complement(16978..17241)
                     /locus_tag="PK144_01745"
     CDS             complement(16978..17241)
                     /locus_tag="PK144_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016390382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64476.1"
                     /translation="MLSYVDCVGFAGLTEDEIEAIAEHEHVPEIVAAEFGCCLCETTE
                     GVPLMRRILLDDIRHAEAHGAVLHALHLRAVLAKFEAGHEVLH"
     gene            17460..17900
                     /locus_tag="PK144_01750"
     CDS             17460..17900
                     /locus_tag="PK144_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNF64477.1"
                     /translation="MNTGDKTAGTTYTISQLAREFDVTTRAIRFYEDQGLLIPERVGR
                     RRIYARRDRTRLKLILRGKRLGMTLGETRELFDLFDSSRDEARQLERFLRILGERRDI
                     LVQQQRDIEAVLHEINAAAAECERLLAEHAEPSPQDPAGPTVPA"
     gene            18079..19257
                     /locus_tag="PK144_01755"
     CDS             18079..19257
                     /locus_tag="PK144_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNF64478.1"
                     /translation="MELPSLNFALGDTIDALRDTVRAFAQAEIAPRAAAIDRDNRFPA
                     DLWPRLGELGLLGITAPEDFGGAGMGYLAHMVAMEEISRASAAVGLSYGAHSNLCVNQ
                     IRRNGSAAQQARYLPKLISGEHVGALAMSEPGAGSDVLAMSLRAERRGDHFVLNGAKM
                     WITNGGDADTLIVYAKTDPDAGARGLTAFILEKDMPGFRHGPHLDKLGMRGSNTYPLF
                     FNDCELPVDNVLGGVGNGVRVLMSGLDYERAVLAAGPLGIMQACMDVAVPYLHERRQF
                     GQAIGEFQLMQGKIADLYTTLSACRAYVYALGQALDRLGDQHVRSLRKDAAGAILYAA
                     EKATWMAGETIQILGGNGYVNDYPAGRLWRDAKLYEIGAGTSEIRRMLIGRELFEETA
                     "
     gene            19277..20452
                     /locus_tag="PK144_01760"
     CDS             19277..20452
                     /locus_tag="PK144_01760"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNF64479.1"
                     /translation="MKDPVVIVGQARTALGEFRGALSALGAPALGAVAIRAAVTRAGV
                     DAQAIDAVFMGCVLSAGLGQAPARQAAIAAGLPWSVPCTTVNKVCGSGMQAVMLAHDG
                     LLAGSLQRAVAGGMESMSNAPYLLPKARTGLQLGHGQLLDHLFTDGLEDPFEPGRRMG
                     EYAEDCAAAIGCPREDQDAWALESLRRARAAIDEGAFAGEISGCAVPERGGGTWVDVD
                     QHPLHARAERIARLPPVFRADGSVTAGNASAIADGAAALVLMRASAAANLGLPVLARI
                     RGHATQAQRGWTDAPAAAVRKLCAAIGWRIADVDLWEINEAFAVIAIAALRELGLAHA
                     QVNVHGGACALGHPIGCSGARLLVTLIAALQRHGLRRGIATLCIGGGEATAVAVERG"
     gene            20567..20767
                     /locus_tag="PK144_01765"
     CDS             20567..20767
                     /locus_tag="PK144_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNF64480.1"
                     /translation="MTYAIEDPASPCIGICQMNDAGLCLGCFRTLDEIEHWWDYDPKQ
                     KWTVIAMAEDRQARYLDGETDD"
     gene            20778..21380
                     /locus_tag="PK144_01770"
     CDS             20778..21380
                     /locus_tag="PK144_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNF64481.1"
                     /translation="MPLSPPAPRQLSHTRTVTCRGYEREDGLWDIEGHMTDVKTYAFP
                     NRDRGGEIKAGEPVHGMWLRITIDDHLLVHAAESWTEYSPFNICPEINDAYGRLVGLR
                     IGPGWKKHLKERLGGTQGCTHLTELLGPMATTAFQTVFKRQQKSKEEMRTLRRPPALL
                     DTCHGWSAAGPVVAWLMPQFHRPRPLAAETEADGIGHADR"
     gene            21490..22494
                     /locus_tag="PK144_01775"
     CDS             21490..22494
                     /locus_tag="PK144_01775"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNF64482.1"
                     /translation="MSRRLSPFERLLLLIGCSVAVLALGLSLPFADARHAGIALLRAL
                     LLGGFASAITLGLGLGLMVWQERRAREEWHLRRVQLRARATYATHGSAPEATVVPAPP
                     PAASDTPADTRTASPVAPGSAAVWTTGLLAALDWRRFEELCAGYFRLRGYRTEQRPPG
                     SDGGIHIELYRPDTGDRLLGILLCRACGNGGCTGIRELRELYGMMTAEAAALGLYVSR
                     SGYSAEAVAFAQGKPLRLLDPSGLVALLRKLPAEASAALYAEIVRDDYATPSCPQCGD
                     KMLLRTLTSGPRAGTRFWGCRHYPDCRGLMPLRAENVTVAVPVHAFAEPGRPPRAAAS
                     "
     gene            22539..23147
                     /locus_tag="PK144_01780"
     CDS             22539..23147
                     /locus_tag="PK144_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005901467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNF64483.1"
                     /translation="MLPDEAAELLDFWFGPLPDAGSLDPVRAAHWFLDGRAWDTPIRT
                     RFGALIERAARGELDDWPASPRGRLALILLLDQCPRHVYRATAAAFASDRLAQAQALT
                     GIVRGDDLALHPVERLFHYLPLEHAEDAVLQAASVAAFTRLHAETPPALQQQSAGWLD
                     YAERHRAVIERFGRFPDLNALLGRQTTAAEQDFLASPGSSFL"
     gene            complement(23594..24268)
                     /locus_tag="PK144_01785"
     CDS             complement(23594..24268)
                     /locus_tag="PK144_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNF64484.1"
                     /translation="MNPCLRLGLVLVAALSTACTTLPLDHEFRPVMPTVMTTVPQARP
                     RVADGSIYRAGTDLRLFESRTARRIGDVITIRLAERTNASKKADTKLDKSSNASVAAP
                     AVLFGKVYPKAEIDLSSERDFEGSGSADQSNSITGTITAVVTEVYPNGNLQIRGEKKV
                     TLNQGDEFVIVQGVVRPDDIAADNSIASGQVADAQITYAGKGAVADANTAGWLTRFLI
                     HAFFPI"
     gene            complement(24280..25065)
                     /gene="flgG"
                     /locus_tag="PK144_01790"
     CDS             complement(24280..25065)
                     /gene="flgG"
                     /locus_tag="PK144_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNF64485.1"
                     /translation="MNPALWVAKTGLDAQQTRMDVIANNLANVNTTGFKKSRAVFEDL
                     LYQNVRQVGGQTSQNTTLPSGLMLGTGVRVVATEKMHTQGNVINTDNALDVAINGRGF
                     FQIQLPDGTTAYTRDGSFQINDQGQIVTSEGYPLLPNLTVPNGAQSLTVGSDGVVSVT
                     LAGQIAPQQIGNLQIADFLNPAGLQPIGENLFLESGASGAAQLGDPRTNGLGALQQHM
                     LESSNVNVAEELVSMIETQRAYEVNSKAIQTTDQMLQYLNQNV"
     gene            complement(25080..25814)
                     /locus_tag="PK144_01795"
     CDS             complement(25080..25814)
                     /locus_tag="PK144_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HNF64486.1"
                     /translation="MDRMLYVAMNGAQQILRAQTVVSNNLANAATTGFRQDLATMRSQ
                     PVIGDGLPTRVYAMAERSGVDFTAGGIETTGRDLDVALKTDGYLAIDDGRGGEAYTRA
                     GNLRIDGGGRLMTADGHVLLGERGPIVIPPVDKLDIAGDGTISILPSGETNVVALDRI
                     KLVRPAANDLIKGADGLLRSKAGGPFAPDASVQLVPGALEGSNVNTAHALVSMIELAR
                     RYETQVKLMSAADDAARSTDRLMSLS"
     gene            complement(25860..>25961)
                     /locus_tag="PK144_01800"
     CDS             complement(25860..>25961)
                     /locus_tag="PK144_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNF64487.1"
                     /translation="QLVNMIQAQRNFQANAQVIQTADQLTQTLLQIR"
BASE COUNT         4151 a         9207 c         8611 g         3992 t
ORIGIN      
        1 ggcaggccgg cgcgcagcac catgccgttg ccgtcgccgg tgcaggggtg ggcggaggtc
       61 gccgagaagt atgcgcggcc gtaaccgccg gtggcgagca cggtctgctg gccgcggaac
      121 acatgcagcg tgccctcggc gagatcccag gccagcacgc cgcggcactc gccgtcttcc
      181 atcagcaggt cgagcgcgaa gtattcgatg aagaactcgg cgttgtggcg cagcgactgc
      241 tggtacagcg tatgcaggat cgcgtgaccg gtgcggtcgg cggccgcaca ggtgcgctgg
      301 gcctgaccct cgccgaagtt cgtggtcatg ccgccgaacg ggcgctggta gatcttgcct
      361 tcctcggtgc gcgagaacgg cacgccctgg tgttcgagct cgatgatcgc cgggatcgcc
      421 tcgcggcaca tgtactcgat cgcgtcctgg tcaccgagcc agtcggagcc cttgaccgtg
      481 tcgtacatgt gccactgcca ggagtccggc cccatgttgc cgagcgcggc cgagatgccg
      541 ccctgcgcgg cgaccgtgtg cgagcgggtc gggaagacct tggtcaggca ggcggtgcgc
      601 agacccttct gcgccatgcc gaacgtggcg cgcaggccgg cgccgccggc accgaccacg
      661 accacgtcgt aggtgtgttc gatgatcttg taatcggtcg tagccatgaa aatccttagc
      721 ctccgaaggc gatcttgagg acggagatga cggcggtggc accggcgacg atgacgacaa
      781 gattcatcgc cagcagcgca ccgagcttca ccagctcctc atggacgtag tcctcgatca
      841 ccacctgcag gccgagccgc gcgtgataga acatcgccgt cagcaggacg acgagcagcg
      901 cggtgttgaa cgggcgcgag acccaggcct tcatcgtcgc gtggtcggca tgggcgaccg
      961 acagcagcga taccgcgaac cacagcacca gcggcaccag gatcacggcg ctcaggcgct
     1021 gcagccacca gtggtggaca ccgctacggg acgaaccgag gccgcggaca tgggccagcg
     1081 gcgtgcgcag actgctcttg ctcatcgtgt ctcccccctc aggtcacgat gatccagcag
     1141 acgaaggtca gcgccgtcgc ggcgccgaga accgtcttgc cggtcttgta aacggtcgga
     1201 atgtcgaagc cgtggccggc gtcccacagc aggtgccgga tgccgttgca caggtggtag
     1261 aacagggccc aggtccagca gaacaggaac agctggccga gcatcgaacc gagcacgtgc
     1321 tgcgcacgct cgaaggcttc agggccgacg gcggcggcgg ccagccagta gacgatcagc
     1381 agcgttccga tcgacagcgc gacaccggtg ccacggtgca ggatcgagag gaccgaggtc
     1441 atctgcggtt tgtagacctg aagatgcggc gacaggggcc gcttgtccgt tgccatgtat
     1501 gtccacctcg agatgcgggc ttgacgctgg tttatcgttc tgtccggccg tctggcgacg
     1561 gccggcgatt ttgccggtgt ctaatgtgtc gatggaacgc attagagtcc ggcaccccct
     1621 cggcctgcgg ccaccgtaat ccggtcactg aagcgtcatt gcaaccggaa tccgggataa
     1681 ggaattcgaa tttcgtgatt ctgaattccc ggaccgtcaa agccaaaaaa cgcgcgcaat
     1741 atcgcaaacc gccgcccgcc cgggcaagca tggcagcagc ggaatgcggt tcagaaatcg
     1801 atgcagcgac cgttcttctc ccagtcgcca tagcgggtcg gttcagggcc ggcgcgacca
     1861 ccgtactcgg tggtggtggc gggcggcggc gtttcgtcga ggctgtcggg atgcccggca
     1921 ggggcggccg gcggtacagg ttcgactgcc ggcgacggct cgtcgcacgt tacgctcata
     1981 tgtccttgat ctccgccgca aaagctgtcg gccgtcacga cctgattcca ggatcacggt
     2041 cgccggtacg ggcttttttt cgatgatcgc gcattatgcg gaccgggcag tacgcttgca
     2101 acgcaagggc ggcgccaccc gggcgccggg atgacgccat ccccggccct ccgcgccgcc
     2161 ttccccttcg ccgcccgcca gatccccgaa gatgaccgac gataccgatc cgactgccga
     2221 cacctgcctc catcccctgc ccgcccccgg cctgatccgc gtggccggtg ccgaggcatc
     2281 cgccttcctg cagggccagc tgacgcagga cgtgcgccgc atcgacgcga cgacgacgct
     2341 gcgcgccgcc tgctgcaacc cgaagggccg cgtgctggcg acgctgcggc tgttcgcgcg
     2401 cgatcaggcg ctgtggctct ggctgccggc cgagcgcgcg gccgcgctgg cacagcggct
     2461 gaagatgtac gtgctgcgcg cgaaggtgga actgagcgac gtgagtccgc agttcgccgc
     2521 cctcggcgtg agcggtgcag gcgccggcgc acggcttgcc ggggtactgg gcgtgccggt
     2581 gccggagcag gcggaccgcg cgctgacgca cgatggcctc accgtgctcg gcctgcccgc
     2641 agcgccgccg cgtctggccg tgatcggcga tccggcccgc atcgacgccc tgcagcaggc
     2701 gctgctcgca gccggggcgc gtgccgccac ggccgaagac tgggcggcgc tcgacatcgc
     2761 cgccggcatc gccgaggtgc acggtccgct ggtcgaagcc ttcgtgccgc agatgctgaa
     2821 cctcgaccgc accgacggca tcagcttcag caagggctgc tacaccggcc aggagatcgt
     2881 cgcgcgcgtg cattacctcg gccagatcaa gcagcggctg taccgggcgc acgtcgatgc
     2941 ccccgcggca ccggccgtgg ccgcggcgct gatcgcggat caggccggtc cgaacgagca
     3001 ggccggccag atcgtcagcg tcgccgccga ccctgcgggc ggctggcaga tgctggccgt
     3061 cgtcacgaca caactcgccg cccgggacgc cgtccggctc gccgacggca cgccgctgcg
     3121 cctgcagccg ctgcacgccg gacaggacac ctgactcgga cgttccgcgc gcggccggcc
     3181 cgggccggat tacggaggcg gttccatgga ggtcatggag gcctcccgca catcattacg
     3241 cccgcacatc gctgaaatcc tgcggacaga tgattacggc cccgccccgg cagcctggcg
     3301 ccatgctcat tcgccgccat tgcggaagcc ggaaatccgg ctgattccgc aggccctcat
     3361 ctgcagacgc gatcagacgt ttgacggcat aatcatcaac ttacaaaata ttaagcttgt
     3421 aattccgccg ccgccccgct gaaacggccc ggtatttccg caagcgccga tggcaggcgg
     3481 aaccggaagc tggcgtttta cggcacgcac tttccggaag atgtgcgctg cacattcctt
     3541 ccggaaacag gtatcggagt cgccggtgaa ttccaccgga agccgggatg gcttccgtgc
     3601 ccggccggcg cagccgtgca tcccgagagt cagttgccat gggcggcagc cgcatgaaaa
     3661 ccgggggccg gacctgcacg gaccggcccc cggggtcggg cggattcagg cctgccgggc
     3721 gcacggcccg ccctgcgggc cccgccgccg gcagcgcctc acggccgttc cagcgccagc
     3781 gcgatgccct gaccgccacc gatgcagagc gacacgatgc cgcgctgccc gccggtgcgc
     3841 gccagcgcgt gcgcgaggcg cacgatcagg accgcgccgg tggcgccgat cgggtgaccg
     3901 tgggcgatgg cgccgccatc gacgttgaag cgcgccggat cgatgtcgag ctgctgctgt
     3961 gcgaccagcg ccacggccgc gaaggcttcg ttgacctcga cgcggtcgat ctcgccgacc
     4021 gaccagccgg tgcgcgtcag caccttccgg atcgccggca ccggcccgta gccgaacaga
     4081 cccggttcga cgccgaccac agcgctcgcg cgcagcacca gcatcggcgt cagcccgtgc
     4141 gcatcggccc aggcgcgctt gcagaccacc atcgccgccg ccgcggcatt gacgccgggc
     4201 gcgttgccgg cggtgatcgt gccaccgtca cggaacgccg gcctgagctt cgcgagcgat
     4261 tcgagcgtcg tttccgggcg gttgccctcg tcggcggcaa acagcgccgg acccttcttc
     4321 gtgcggatct ccagcggctc gatctcggtg acgaagtggc cggcggcctg cgcggccgcg
     4381 aagcgctgct ggctgcgcgc ggcgaaggcg tcctgatcgg cgcggctcag gccgaacttc
     4441 gcgaccagat cctcggtatg ccagccggag tgctcgccgg agaaggcatc gttcaggccg
     4501 tcgcgcagca tcgcatcgat cgcctgggcg ttgcccatgc gcgcgccgtg gcgcatcgcc
     4561 ggcaacagat aaggcgcgcg gtccatgttc tcggtgccgc cggcgatcac gacctcggcc
     4621 tggccggcga ggatttcggc cgcggcactg gcgagcgcct gcgcgccgga gccgcagaca
     4681 cggttgacgg tcagcgccgg cacgccgacc ggcagcccgc cgccgatcgc ggcctgacgc
     4741 gcgaggttca tgccgttgcc ggcctggatg acgttgccga acaccaccga gtcgacctgc
     4801 gccggatcga gtccggcgcg gcgcaggctg gcggcgatca cgtgcgcgcc gagcgcggcg
     4861 gccggcacgt cgacgaggct gccgccgaag cggccgatgg cggtgcggac cggggcacag
     4921 acgacgatat cgtcggaacg ggcggatgac gaactcatgg cacggcactc ctcaggaccc
     4981 ggcggccggg tcggtcaatg aggcacggcc gctcgtgacg gccgcaccgg gggatgcggg
     5041 actgacagcg gatcggtgaa ggcatccgtc agtcactttc atgttgaaag taagtttgag
     5101 gttcatcccc ttcgccagca agccggtcgc ggactggcag cggtgcagcg atcgccgtgc
     5161 cggcaggccg gcgacccgcc tccggccggc cctgggccgg cggcgatcac ggtccggcgg
     5221 ccggtgcgca gctctgcatc tcgcctgccc ggagcccgac cgctgcccgc tgcgggcagc
     5281 ggaacacgct gcgcaggcag gctgccgatg ccacggtccc gagcgtcgca aaccctacat
     5341 tacccactgc tgcactcggc attgtcggct gaacaaatcc gacacagacc atcatatagc
     5401 tgtttttgaa cgattatttt gttggcacgc ctgatgctta ggtcctgccc atgaaccgga
     5461 ggaccggaac catggaccgc agcatcctga tccacgacac caccttgcgc gacggcgagc
     5521 agacggccgg cgtggccttc aaccgggctg aaaagctcgc gatcgccgcc gccctcgatc
     5581 tcgccggcgt gcaggaactg gagatcggca tcccggcgat gggcgccgag gagcaggacg
     5641 acatgcgcgc gatcgtcggc ctcggcctgc agacgacgct ggtggcctgg tgccggatga
     5701 gccgcaagga cctcgacgcg gcgctggcca ccggggtgcg cgccgtgaac ctgtcgacgc
     5761 cggtctccga cacccagatc cagctcaagc tcggccgcga ccgccgctgg gtgctcgcga
     5821 acgtcgcgca gatggtggcg atggcccgcg acgccggctt cgacgacgtg cgcgtcggcg
     5881 gcgaggatgc ctcgcgcgcc gatccggact tcgtcgccga gatcgccgcg accgcggccg
     5941 cggccggtgc ccggcgcttc cgctacgccg acacggtcgg cctgctcgaa cccttcggca
     6001 ccttcgagcg catcacccgg ctgcgggcgg cgacggcgat cgaactcgaa atccacgccc
     6061 acgacgacct gggcctcgcc accgccaact cgctggcggc ggtgcgtgcc ggggccgact
     6121 tcgtcagcac cacggtcaac ggcctcggcg agcgcgccgg caacgccccg ctcgaagagg
     6181 tcgcgacggc gctgtcggtg ctgcacggcc gcgactgcgg cgtgcgcctc gcgcagctgc
     6241 cggcgatctc gcagctcgtc gccgaagcct cgggccggcc ggtgccggtc ggcaagagcg
     6301 tggtcggcga tgcggtgttc acgcacgaat ccggcattca cgtcgacggc ctgctgaagg
     6361 acccgcgcac ctatcagggc ctggacccgg cccgtctcgg tcgccatcac cggctggtgc
     6421 tcggcaagca ttcgggcacg gcctcggtgc gccgcgccta cgccgagctc ggcctgcgcc
     6481 tggccgagga cgaggtgcag cgcctgctcg gacgcatccg cgtgcacgcc ggcagcacca
     6541 agcgcgcacc gaccgccgac gaactgctcg gcttctggca cgagacccgt gcgccgcggc
     6601 cgtacgtggc cggcacgctg aactgatcag gaggcttcgt gatgcgcgct ccgtccgatt
     6661 ccctgtccgc cgccggtatc ccgacgatga cccagaccgt ccgcccgggc ctgctcgccc
     6721 gcctgcgcga ggacgtgctc gccgtccgtg cccgtgatcc ggccgcgcgc agtgccttcg
     6781 aggtgctggc cacctacccc ggcctgcacg cgctgatcct gcaccgcatc gccaacgggc
     6841 tgtggcgccg ccagtggcgc ttcacggcgc ggctgctcgc cggcctcgcc cgcgggctga
     6901 cgcagatcga catccacccg gcggcgacca tcggccggcg catgttcatc gatcacggct
     6961 gcggcgtggt gatcggcgag accgccgaga tcggcgacga cgtgacgctg taccagggcg
     7021 tgacgctcgg cggcaccagt ctgtcgcgcg gcaagcgcca cccgacgctc ggggacggcg
     7081 tcacggtcgg ggccggggcc aaggtgctcg gtgccatcga gatcggcgcc ggtgcgcgcg
     7141 tcggggcgaa ttcggtcgtg atccgcgacg tgccggccgc ggccaccgtc gtcggcatcc
     7201 ccggtcgtgt cgtgccgcgc ggcgacacgg ctcgggccga cggcatcgac ctcgatcacc
     7261 acctgatgcc ggacccggtc ggccgtgcgc tcgcctgcct gatccagcgc atcgacgacc
     7321 tcgaagcccg gctcgccgcc gccggtccgg ccatccccgg actcgatcac ccgcatgcct
     7381 gcaccgactg cggcgatgcc tgcgatcagg cccgccatgc cgccgccgcc caccccgctg
     7441 ccctgcactc ctgaggaatc catccatgag cacgctgagc gatcgtctgc agaccctgtc
     7501 ggccgccgat gaattcttcg agctgttcaa gatcgatgtc gacccgcacg tcgtccatgt
     7561 caaccggctg cacatcctca agcgcttcca cgactacctg aagcgctcgc cggtccaggc
     7621 ggacctctcc gaagccgacg aattcgccgc ctaccgcgag cggctggtgc aggcctacct
     7681 cgatttcacc gtgtcggacg cggtcaccga gaaggtcttc aaggtgttcc gtcaggcgca
     7741 gggccagggc ttcgtcggtc tcgaccagat cgaacgcatc gaacgacctg cgacctgatt
     7801 tccccttatc cacgactgcc acgtacccga cgagccggag gccacccatg cacatcgtcg
     7861 tctgcatcaa gcaggtaccg gacagcgcgc agatccgcgt gcacccggtc accaacacca
     7921 tcatgcgcca gggcgtcccg gccatcatca acccctacga cctgttctcg ctggaagagg
     7981 cgctgcggct gaaggaccag ttcggcggca aggtgacggt gatcacgatg ggtccgccgc
     8041 aggccgagga tgcgctgcgc aaggccctgt ccttcggcgc cgacgaggcg atcctgctga
     8101 ccgaccgcgc gtttgccggt gccgacacgc tggcgacctc gtgcgcgctg gcccaggccc
     8161 tgcgccagat cggcgagaag gagccggtcg acctggtgtt caccggcaag cagacgatcg
     8221 acggcgacac cgcccaggtc ggcccgggag tggccaagcg cctcgacctg cagctgctga
     8281 cctacgtcgc gaagatcgac gagctcgacc tcgcaggccg cacgatcacc gtgcagcggc
     8341 gcgccgaggg cggcgtgcag gtgctcaaga cccgcctgcc ggcgctgatc acgatgctcg
     8401 aaggcaccaa cgagatgcgc ttcgcgacca tgaccgacat gttccgcgcc gcgcgctcgc
     8461 cgctgacggt ctggaacaag gacgacgccg gcatcaccga catcagcaag atcggcctga
     8521 agggctcgcc gaccgtggtc agcaaggtgt tcgcgccgaa gccgaagtcg gtgccggcgc
     8581 agatgctcga catcggcggc aagagccaga ccgagatcgc cgacctgatc atcgacaccc
     8641 tgtccgcgaa gcatgcgccg ctgtacggcg agctgttcga ccgcgcgctc tgaaccccac
     8701 gactcccgga gatagccgac atgagcgaag ccccgaagcc ggccgccccc gcagcgcgcg
     8761 gccgcaagaa actcgaactg gacccgcggc tggccgcgta caagggtgtg tgggtgttca
     8821 tcgagcatga acgcggcacc gtgcacccgg tgtcgtggga gctgctcggc gaaggccgca
     8881 agctcgccga caagctcggc gtggagctcg ccggcgtgct gctcggcggc cccggggatc
     8941 cgctcgatgc cttcgccgcc accgcccaca cctatggcgc cgacctcgtc tacatcaccc
     9001 gcagcccggt gctggccggc taccgcaacg agccgtacac gaaggcgctg actgcggtcg
     9061 tcaaccagtt ccagcccgag atcctgctgc tcggcgccac cgtgcagggc cgtgacctcg
     9121 ccggttccgt ggcgacgacg ctggcgaccg gcctgaccgc cgactgcacc gaactcgcga
     9181 tcgatccgga caaccgctcg ctcgccgcga cccgtccgac cttcggcggc tcgctgctct
     9241 gcacgatcca gacgctgaac taccggccgc agatggcgac cgtgcgtccg cgcgtgatgt
     9301 cgatgccggc gcagcagccc gagcgcagcg gccgcgtgct cgaggcgccg gtcgagctgg
     9361 tcgaggacga catcatcgcc aagctgcttt cgtacatccc ggacgatgcc accgaccagg
     9421 cgcagctcgc ctacgccgac gtgatcgttg ccggcggccg cggcctcggc aagcccgaga
     9481 acttcaagct gctctgggat ctggccgagg tgctcggcgc cgaggtcggc ggctcccggc
     9541 cggtgatcca gaacggcatc ctcagtgcgg accgccaggt cggccagacc ggcaagaccg
     9601 tgcgcccgaa gctctacatc gccgccggca tctccggcgc gatccagcac cgcgtcggca
     9661 tggaagcctc cgacgtcatc atcgccatca acacggaccc caaggccccg atcttcgact
     9721 tcgccaccta cggcatcgtc ggtgatgcgg tgcagatcct ccctgccctg accgcagcct
     9781 gccgcgcccg tctggcgatg aagcgcgccg gctgaaggag accgccatgg aaaaattcga
     9841 cgtcatcgtg gtcggggccg gcgctgccgg caatgccgcc acctacaccc tggccaaggc
     9901 cggcctgaac gtgctgcaga tcgagcgcgg cgagacctcg ggcaccaaga acgtgcaggg
     9961 cgcgatcctg tacgccgatg cgctggagcg catcatcccg gacttccgcg acgacgcgcc
    10021 gctcgaacgc cacatcgtcg agcagcgcat ctggatcatg gaggacaagt cgcacgtcgg
    10081 tacgcactac cgctccgact ccttcaatga ggaacggccg aaccgctaca cgatcctgcg
    10141 cgcgcagttc gacaagtggt tcagcggcaa ggtcagggag tccggcgcgc tggtcatcaa
    10201 cgagaccacg gtgctggagc tgctgaagaa tgacgccggc cgcatcgtcg gcgtgcgcac
    10261 cgaccgcgtc ggcggcgaca tctacgccga tgccgtgatc ctcgccgatg gcgtcaactc
    10321 gctgctggcc cgcaaggccg ggctgcgcga ggagatcgag gcgaagaacg tcgcgctcgc
    10381 ggtcaaggaa gtccacttcc tgccgcagga cgtgctggag agccgcttca acatccagga
    10441 agaggaaggc gtcgtcatcg agatcgccgg caccatcacc cgcggcatgg ccggcacggc
    10501 cttcctgtac accaaccgcg agtcggtcgc gatcggcatc ggctgcatgc tggaggactt
    10561 caagggctcg ggcgtgtcgc cgtacacgct gctcgaagac ctgaagaacc acccgtcgat
    10621 caagtcgctg atcgccggcg gcgaggtcaa ggaatacgcc gcgcacctga tccccgaggg
    10681 cggctacaag gcgctgccga agctcgccgg cgacggctgg ctgatggtcg gcgactccgc
    10741 cggcttcgtg aatgccgtgc accgcgaagg ctcgaacctg gcgatgacca gcggccgcct
    10801 ggccgccgag acgctgatcg cgctgaagac cgccggcaag ccggcgacgg ccgcgaacct
    10861 gcaggcctac gtcgaggcgc tcaacgacag cttcgtcatg aaggacctgc gcaagtacca
    10921 gcacgtgccg gccctgctcg aagagaagca gttcctgacc acctacccga ccctgctcaa
    10981 ccgcgccgcc gaaaccatgc tgcgcgtcga cggtgtcgac aagaagacca agcagcgctc
    11041 ggcgctcggc gcgttcgtgc aggaccgctc gctgttcggg ctgatgaagg acacctacaa
    11101 gctgtggagg gcattccggt gagcaaggtc gaggaaaagc tgttccagaa ccgctaccgc
    11161 gtcgatgaag gccgcccgca catccagatc atcgatcagg agaagtgcac ggaatgcggt
    11221 acccaggact gcacgtatct gtgcccggcc gcctgctacg ccaaggccga ggacggccgg
    11281 catgtggtgc tgaccaccga cggctgcctg gaatgcggca cctgccgggt gatctgctcg
    11341 gagttccgca acgtcgcctg ggaatacccg cgcggcggct acggcatcct gttcaagttc
    11401 ggctgaggac aaggccatgc tgaccccgtc cctgccgctg cgtgcggtcc cgctcgcgga
    11461 gtacccgttc ggactcggcg tcaccgatgg cgccggcttc cgggcgctgc gcgaggcggc
    11521 agcggacgat gccgcctcgg ttgccggtgc ctatctcgaa caggcggcgg ctggcgtcga
    11581 gccggcccgc cgcgagttcc tgctctggag tgcgcacgcg ctggcaccga atcatctgcc
    11641 cgtgtacttc gcgctgtaca agttcctgtt ccaccagaag cggctggtcg atgccgaacg
    11701 cgtggcgctg atggcgctcg gcgagtcggc ccgccagggc ggcttcccgg tgcgctggaa
    11761 cgaactgcgc cactggaccg cggactggtc cgacggcccg ggccgcttct atctgttcag
    11821 cctgaaggcg ctggccttca tccggctgcg gctgcacggc ctgaaggccg ccggaccgct
    11881 gctcgagaag ctgcacgaac tggatccgca ggaccaggtc ggcggctcgg tgatcaccga
    11941 tctggcccgg gcctgcagcg cagcgggctg agccggacca cgaatcgaaa cgctggaccg
    12001 gtatcctcac gcccggtccc gcttccgtcg ccggccgatc catggccgcg gcgacgcgcc
    12061 tttcctaccc gacggtggag tacttcatgg actgcatcaa ccgttttctc gcccacgcga
    12121 tcgcgctcga aggctcggcc gccgggcgct acgaagacct ggccaactcg atgagcgcgc
    12181 tcggtgacgc cgaggtcgcg gcctttttcc ggcagatggc cgagttcgcc cgcatgcatc
    12241 aggccgaggc catcgaccgc gcggggttcc gcgatgtcga gcagccgccc gaggacggct
    12301 acgaatgggg gccgggcgaa agccccgagg acgccgcctg ggccggcgtc gacagcttcc
    12361 tcgacgtgac cgcggcactg gaactcgcgc tcgaagccga gcgcagcagc gaggccttct
    12421 accggcgcgt cgcgcgcgag acctcggatg ccgaggtgcg cggcttcgcc gaatccttcg
    12481 ccgaggagga agtcggccac gtcaacgcgc tgctcgcccg gctggagcgg cgcgcgtcgg
    12541 tctgatcccg ccccgcgcac tccgcctccg atcgccccgg cccccgtgcc ggggtttttc
    12601 tttgcatgcc ccggccgggt cgcgggccgc gaccggccac tgccgccgcc gcgacatgcc
    12661 gtggccggca gcggcgatgg ccgggagccc cgcagccggc gagggaaaaa cggctgccat
    12721 ccactcaaaa aaatccattt aaatcagcaa cctgatgaaa ttaacgccag cttttccccg
    12781 gctggcatca ctcctgccca taccggttca tcccagcccg gacgcccgaa ggcgccggct
    12841 gcggacgcgg caagcccgcc gccatcccat ccgcctgccc tgcaatccga accggagatg
    12901 agcaccatga acctgaacat ccgcaccctg atcgccaccg ccctgatcag ctttgccgcc
    12961 gccggcagcg tcaatgcgct ggccagcgaa gccgacagcc tgccgatgcc gtcctggaat
    13021 cctgagcaga cggtgtacgg cagcccgatc ggcctgtcgc aggctccggc cgcgatgacc
    13081 cgcttcgagg cacgcatgag cgcgaccggc agcggccacg agctctgcgc ggcacgcgtc
    13141 gcgctgaacc cgaacgccgg gcgcaatgca tccctgcagg cctgctgaac ggctcccgca
    13201 gcggttcacg ccggccgatg cgggaacctt tgccgcggca gcggcatcgg agcaggcgtt
    13261 acccgcccga cggactgcct ctgcgaggta ccaatcatgc gaggtttcgc cccttccctg
    13321 gcccgcgccg tcctgcccgc agcctttgcg ctcgcgagtg ccatcggcag catcgccctc
    13381 gccgccgggc ctgccgccgt cccgtcccgc gacgccgcgc tgctcgcccc gcagctgccg
    13441 ccggcgctgc gggacaccga ccccgccctg ccgacgctga cgagtccgga cgcggctccg
    13501 gcgcgccggc cggcggccga caacggtcgc gtcggcgacg acacgacttt ctgcaacgtg
    13561 ttcctgaacg aatcggagcg cgagcgcagc ggcgcaaccg gcttctgctg agccctgccg
    13621 gcggccagcc ggaaaaattt ccgccccggc cgcatccgcc gcgctccctg cagcgcatat
    13681 cgccctgcaa gccccgggaa tccaagcccg cgaacccacc cgagagacga gaaaccacca
    13741 tgaacctcaa gaccgccatc gctgccagcc tgctgaccct ttccgtcgcc accatcgccc
    13801 atgccggcga gcgcgattcc tatccgcagc cgtcctggga tccggacacc agcgatcacc
    13861 tgcaccgccc ggccgcaccg ctcggtgctg cgaatgcctc gatcgacgag cgcatgcgtg
    13921 ccaccggcag cagcaccgac ttctgcatca ccgagctgta tccgctgaat ccgcgcggcg
    13981 gcaacaacgg ccacgccggc atctgctgaa ccggtaccgg cgctgtggag gtaccgcgag
    14041 ccgctgcagc gccggtttcc gttcccccga ggctcacgag ccttcccccc gaggcaaacg
    14101 aggactccga catgaacgtc aagaccgcca tcgccgccag cctgctgacc ctgtccatcg
    14161 ccggtgctgc ccatgccatg gaccgttacg acaccgggtc acggcccggc gcctatccgg
    14221 aatatccgtc cgagaccggc aacggcatgc accagcgcgc cccggtcaca cagagcggca
    14281 gccccggctt tgaccagcgc atgcgtgcca ccggcagcgg cttcgattac tgccagcagg
    14341 cgctgtaccc gctggagccg agcagtggcc gcaacgtgct cggcggtacc tgctgatctg
    14401 cattcatgac ggcgcgggcc cgagtcgccc gcgccggccc tgccgcctga aagcctcacg
    14461 aggacactgc catgaatctg aagaccgcca tcgccgccag cctgctgacc cttgccgccg
    14521 ccacggctgc ccatgccggc gacagcgatg cctatccgcg tccggactgg aacccgaacg
    14581 ccagcgacta cctgtacaag cagccgaacc cgtacgggat gagcacggct gcgtcgatcg
    14641 atgaacgcat gcgcgcaacc ggcagcggga cggaattctg cgtcgagccg tatgcgctga
    14701 atccgcgcga cggcagcaac agccacgccg gcgtctgctg atcgccctgc ctgacttcct
    14761 ttctcccgat ccgggttttc cgttcacgcg gaacccggat ttttttatga actgcatccg
    14821 atgcgtgcgg cgcgcccaaa aaaaataccg ccgctccggt taaggaggcg gcggttgcgg
    14881 tgtgccggcg gcatccggtc ttatgagccg gcgggcaccg tatggctgcg ggcggtggga
    14941 cgggacagaa cctgaatcag gcagtctgga actcagcgcg cgagccggcg cttgacgtag
    15001 ctgcccggag ccggttctag cacggcgagc gcaccatcgc cagcgtggcg tgccggtacg
    15061 cgcgtcgcat cctgctgatc cagccaggca tcccagtccg gccaccacga tccggcggtc
    15121 tgcgtggccg tggccagcca ctcgtcggcg tcgagctcca gacgcggact ggtcagatag
    15181 ccgtacttct tcgcggccgg cgggttgatg acaccggcga tgtggccgga accgccgagc
    15241 acgaaacgca ccggcccgcc gaagaggcgc gtgccggcat acgtggattt ccacggcgcg
    15301 atgtggtcct cgaccgccga caggaaatac accggcgtgc gcacctgacg caggtcgatc
    15361 ggccggccgc acagcgtgat gccgccgggc tcgcgcaggc ggttgtgctg atacatgttg
    15421 cgcagataaa aactgtgcat cgccgccggc atgcgcgtcg aatccgagtt ccagtacagc
    15481 aggtcgaacg gaaacggatc cttgccgagc aggtagttgt tgaccgcgaa tgaccagatc
    15541 aggtcattgg cgcgcagcat gttgaaggtc gacgccatct cggcgccgtc gagatagccc
    15601 tgcttctgca tgcgcctctc gatggcatcg agctgctggt cgtcgatgaa cacttccagt
    15661 tcgccgggcc gggcgaaatc gagcatggtc gtgaacagcg tggtgctggc gatcggctga
    15721 tgatcgccgg ccgcatacca ggccgtgctc gccgacagca gcgtaccgcc gaggcagtag
    15781 ccgacggcat tgacctcgcg ctcgccggtc gcccgttcga tggcctgcag cgccgccgtg
    15841 atgccgtgtt cgaggtaatc atcgaaaccg cgggccgcgt agctggcatc cggattgacc
    15901 caggagatca cgaacaccgt gttgccctgc gcgacggccc agcggatgaa gctgttcttg
    15961 tcgcgcaggt cgaggatgta gaacttgttg atccacggcg gcacgatcag cagcggccgc
    16021 ctgcgcaccg tgtcgccgag cggcgcgtac tggatcagct gcatcatgtc gttctggaac
    16081 acgaccgagc cgggcgtcgt cgcgatgttg tcgccgagcg tgaaggcgtc gaggtcggtc
    16141 atgcggatgc tgagccggcc cttgccgcgc tcgatgtcgc gcatcaggtt gcgcagaccg
    16201 cgcagcaggc tttcgccgcc ggtgcggaag atctcgcgca gcgcctcggg gttggtcagc
    16261 gcgaagttgg tcggagcgag ggcgtcgatc agctggcgcg tgtagaactc gaccttgcgc
    16321 gcgtgctgct cgtcgatgtc ctcgacgccc ttcatggttt ccagcagcca gcgggcgctc
    16381 agcagatagc tctgcttgat gtgatcgaag accgcgaatt cggtccaggc ctcgtccttg
    16441 aagcggcggt cgccgcgttc gggctggatg atcgggctga cgcgggtgcc ggacagcgcg
    16501 ctgcaggtcc tggcccacag atcgaggctg tcgacccaca gcgccgtctg ccggtcgagc
    16561 agcttcagcg gatcgcgcca caggcgcagc gtcgcctcgc cgaaggcgcg accgatgccg
    16621 agttcatcgc cgagccactc cggcacgcgc accggcttgc ggatgcgctc acggaacaga
    16681 ctgccgatca gacgatcccc gctggcgacg atgtcgtgca gcgtcgaggt gatcgccagc
    16741 ggatcgggaa cctggatttc gaatttctcg gccacaccca tcttcgcccc tccggcagga
    16801 cccggcacgc ttggcgttta cgtaaacgca aacggccggg catcgggcaa gtaaacgcca
    16861 gcggatgggg cgattcaaga caacggacca tgacagcagt cgcgccaccg gcggacggtg
    16921 gtgcgactcg gggcgactcg gcaaagattc cgttacaggt aaggacaggc cgtgaggtca
    16981 gtgcagcact tcgtgaccgg cttcgaattt cgccagcacc gcacgcagat gcagcgcgtg
    17041 cagtacggca ccgtgcgcct cggcgtgacg gatgtcatcg agcaggatgc ggcgcatcag
    17101 cggcacgccc tcggtcgttt cgcagaggca gcagccgaat tcggccgcga cgatttccgg
    17161 tacatgctcg tgttcggcaa tggcttcgat ctcgtcttcg gtcaggcccg cgaacccgac
    17221 acaatcgaca taagacaaca tgatggctct cccgacaaac gtgctttccg gcttcctcct
    17281 cccaagcaaa gcacgccggc cggaccttgc aatgaaatgt ttctatgccc atcagcaact
    17341 tgaaagcaaa catcggcctg ccggcctgtt gcagccggag ccggtcgatt gacgtatacg
    17401 tcaatctgtt tcaccttcgc ccgccatccg atccccgccc caacctctgg agagcgagct
    17461 tgaacaccgg cgacaagacc gcgggcacga cctacacgat cagtcagctc gcccgggaat
    17521 tcgacgtgac gacgcgggcc atccgcttct acgaggatca gggtctgctc attccggagc
    17581 gcgtcggccg ccggcgcatc tacgcccgtc gcgaccgcac gcgcctgaag ctgatcctgc
    17641 gcggcaagcg tctcggcatg acgctcggcg aaacccggga actgtttgat ctgtttgaca
    17701 gctcgcgtga cgaggcccgt cagctcgaac gcttcctgcg catcctcggt gagcgccgcg
    17761 acatcctcgt gcagcagcag cgcgacatcg aggccgtgct gcacgagatc aacgccgccg
    17821 ctgccgagtg cgagcgcctg ctcgccgaac acgccgaacc gtcgccgcag gacccggccg
    17881 gaccgaccgt ccccgcctga acggccagcg ccctgccccc tgcccctgcg ccaggcggtc
    17941 gacgttcacg tcaacgtcac ctagcctgcc ggtgactgcc ccgccggccg ggcagccggg
    18001 cctgcaccct gatgcctgca cggccccgcc cctgatccga tcccgcgccg gcccggcccg
    18061 ctccggagca cgacgatgat cgaactgccc tccctgaact tcgcgctcgg cgacaccatc
    18121 gatgccctgc gcgacacggt gcgcgccttc gcgcaggccg agatcgcccc gcgtgccgcg
    18181 gcgatcgatc gcgacaaccg ctttccggcc gatctctggc cccggctcgg cgaactcggc
    18241 ctgctcggca tcaccgcccc cgaggacttc ggcggtgccg gcatgggcta tctcgcgcac
    18301 atggtggcga tggaggaaat ctcgcgtgcc tcggctgccg tcggcctgtc ctacggggcg
    18361 cattcgaatc tctgcgtgaa ccagatccgc cgcaacggca gcgcggccca gcaggcccgc
    18421 tacctgccga agctgatcag cggcgagcac gtcggcgcac tggcgatgag cgaacccggt
    18481 gccggctccg acgtgctggc gatgagcctg cgcgccgagc gccgcggcga tcacttcgtg
    18541 ctgaacggcg cgaagatgtg gatcaccaac ggcggcgatg ccgacacgct gatcgtctat
    18601 gcgaagaccg acccggatgc cggcgcgcgc ggcctcacgg ccttcatcct cgagaaggac
    18661 atgccgggct tccgccatgg cccgcacctc gacaagctcg gcatgcgcgg ctcgaacacc
    18721 tatccgctgt tcttcaacga ctgcgagctg cccgtagaca acgtgctcgg cggcgtcggc
    18781 aacggtgtgc gggtgctgat gtccggactc gattacgaac gcgcggtgct cgccgccgga
    18841 ccgctcggca tcatgcaggc ctgcatggac gtggccgtgc cctacctgca cgaacgccgc
    18901 cagttcggtc aggcgatcgg cgagttccag ctgatgcagg gcaagatcgc cgatctgtac
    18961 acgacgctgt cggcctgccg cgcctacgtc tacgcgctcg gccaggcgct cgaccgcctc
    19021 ggtgaccagc acgtgcgctc gctgcgcaag gatgccgccg gcgcgatcct ctacgccgcc
    19081 gagaaagcca cctggatggc cggcgaaacc atccagatcc tcggcggcaa cggctatgtc
    19141 aacgactacc cggccgggcg gctgtggcgc gacgccaagc tctacgagat cggcgccggc
    19201 acctcggaga tccgccgcat gctgatcggc cgcgaactgt tcgaagaaac cgcctgaacc
    19261 gcgcccggcc ctcaccgtga aggacccggt cgtcatcgtc ggccaggcgc gcaccgcgct
    19321 cggcgaattc cgcggcgcgc tgtcggcgct gggcgccccc gcactcggtg ccgtggcgat
    19381 ccgcgccgcc gtcacgcggg ccggcgtcga cgcgcaggcc atcgatgccg tgttcatggg
    19441 ctgcgtgctg agcgccggcc tcggtcaggc gcccgcccgg caggcggcga tcgcagccgg
    19501 cctgccgtgg tcggtgccgt gcacgacggt caacaaggtc tgcggctcgg gcatgcaggc
    19561 cgtgatgctc gcgcacgacg gcctgctcgc cggcagcctg cagcgggcgg tcgcaggcgg
    19621 catggaatcg atgagcaacg cgccgtacct gctgccgaag gcacgcaccg gcctgcagct
    19681 cggccacggg caactgctcg atcacctgtt caccgacggc ctcgaagacc cgttcgaacc
    19741 cggccggcgg atgggcgaat acgccgagga ctgcgccgcg gcgatcggct gcccgcgcga
    19801 ggatcaggat gcctgggcgc tcgaatcgct gcgccgcgcc cgcgcggcga tcgacgaggg
    19861 tgccttcgcc ggcgagatca gcggctgtgc cgtgccggag cggggcggcg ggacctgggt
    19921 cgatgtcgat cagcatccgc tgcacgcacg ggccgaacgc atcgcccggc tgccgccggt
    19981 gttccgcgcc gatggcagcg tcaccgccgg caacgccagc gccatcgccg acggggcggc
    20041 cgcgctggtg ctgatgcgcg ccagcgcggc ggcgaacctc gggctgccgg tgctcgcccg
    20101 catccgcggc cacgccacgc aggcacagcg cggctggacg gacgcgccgg ccgcggccgt
    20161 gcgcaagctc tgcgcggcca tcggctggcg catcgccgac gtggatctct gggagatcaa
    20221 tgaagccttt gccgtgatcg ccatcgcggc gctgcgcgag ctcggcctcg cgcacgcgca
    20281 ggtcaacgtc cacggcggtg cctgcgcgct cggccacccg atcggctgca gcggtgcgcg
    20341 cctgctggtg acgctgatcg cggcgctgca gcggcacgga ctcaggcgcg gcatcgcgac
    20401 gctgtgcatc ggcggtggcg aggcgaccgc cgtcgccgtc gagcggggct gagtcaggcg
    20461 cacgcgccgg tatcccggca gttcaccgtg acatatggct gctgatgctg cagtgcagta
    20521 gcatcgaccg tccgtgccat ccctgactgt accgccgcct gccgccatga cgtatgcgat
    20581 cgaagacccc gcctcgccgt gcatcggcat ctgccagatg aacgatgccg gtctctgcct
    20641 gggctgtttc cggaccctcg acgagatcga acactggtgg gattacgacc cgaagcagaa
    20701 atggaccgtg atcgcgatgg ccgaggaccg gcaggcgcgt tacctcgacg gcgaaaccga
    20761 cgactgaggc cccccgcatg ccgctctccc cgcccgcccc gcgtcagctc tcgcacaccc
    20821 gcaccgtcac ctgccgcggc tatgaacgcg aggacgggct ctgggacatc gaaggccaca
    20881 tgaccgacgt gaagacctac gccttcccga accgcgaccg cggcggcgag atcaaggcag
    20941 gcgaaccggt ccacggcatg tggctgcgca tcacgatcga cgatcacctg ctcgtgcacg
    21001 ccgccgagtc ctggaccgaa tattcgccgt tcaacatctg tcccgagatc aacgacgcct
    21061 acggccggct ggtggggctg cgcatcggcc cgggctggaa gaagcacctg aaggagcgcc
    21121 tcggcggcac gcagggctgc acgcacctga ccgaactgct cgggccgatg gcgacgacgg
    21181 ccttccagac cgtgttcaag cgccagcaga agtcgaagga ggaaatgcgg acgctgcgcc
    21241 ggccgccggc actgctcgac acctgccatg gctggtcggc ggcaggtccg gtcgtcgcct
    21301 ggctgatgcc gcagtttcac cggccacgcc cgctcgccgc cgaaacggaa gccgacggca
    21361 tcggccatgc cgatcgctga agcgcgcggc atcagcaagc tttacactcg tcaaatcttg
    21421 attcgtcatg actgacatgg cagtttgctt cggtgtcgcc aaggcaccga cctgcaaggg
    21481 aattgcgcca tgtcccgtcg actctccccc ttcgagcgcc tgctgctgct gatcggctgc
    21541 agcgtggccg tgctggccct gggcctgtcg ctgccgttcg cggacgcccg ccacgccggc
    21601 atcgcgctgc tgcgcgccct gctgctcggc ggtttcgcca gcgccatcac gctcggtctc
    21661 ggtctcggtc tgatggtctg gcaggagcgg cgcgcccgcg aggaatggca tctgcgccgc
    21721 gtgcagctgc gcgcccgcgc cacctacgcc acgcacggca gcgcaccgga agccacggtc
    21781 gtgccggccc caccaccggc tgccagcgac accccggcag acaccagaac ggcaagccct
    21841 gtcgcccccg gcagcgcagc ggtctggacg accggactgc tcgcggcact cgactggcgt
    21901 cgcttcgagg aactctgcgc cggttacttc cggctgcggg gctatcgcac cgaacagcgg
    21961 ccgcccggca gtgatggcgg catccacatc gagctgtacc ggcccgacac cggtgatcgc
    22021 ctgctcggca tcctgctctg ccgcgcctgc ggcaatggcg gctgcaccgg catccgcgaa
    22081 ctgcgggagc tgtacggcat gatgacggcc gaagcggctg cgcttggcct gtatgtcagc
    22141 cgcagcggtt acagcgccga ggccgtggcc ttcgcgcagg gcaagcccct gcggctgctc
    22201 gacccgtccg gtctggtcgc actgctgcgg aaactgccgg ccgaagccag cgccgcgctg
    22261 tacgccgaaa tcgtccggga tgactacgcg acgccgagct gcccgcagtg cggcgacaag
    22321 atgctgctgc gcacgctcac cagcgggccc cgcgccggca cccgcttctg gggctgccgg
    22381 cattaccccg actgtcgcgg actgatgccg ctgcgcgccg aaaacgtcac ggtcgccgtg
    22441 ccggtgcatg ccttcgcgga gcccggccgt cccccccggg ctgccgcttc ctgagcggcg
    22501 atccatactg acgggcccgt gtccgatgcg agcccgccat gctgcccgac gaagccgccg
    22561 aactgctgga tttctggttc ggccccctgc ccgatgccgg cagcctggat cccgtgcgcg
    22621 cagcgcactg gtttctcgat ggccgcgcct gggacacgcc gatccgcacg cgtttcggtg
    22681 cgctgatcga acgcgccgcc cgcggcgaac tcgacgactg gccggcgtcg ccgcgcgggc
    22741 gtctggcgct gatcctgctg ctcgatcaat gtccgcggca tgtgtaccgc gccacggccg
    22801 cggccttcgc cagcgatcga ctcgcgcagg ctcaggccct gaccggcatc gtgcgcggcg
    22861 atgacctcgc cctgcacccg gtcgagcgcc tgttccatta cctgccgctc gaacacgccg
    22921 aggacgccgt gctgcaggcc gcctcggtcg ccgccttcac ccggctgcac gctgaaaccc
    22981 cgccggcgct gcagcagcag agcgccggct ggctcgatta cgccgaacgc caccgggccg
    23041 tgatcgaacg ctttggccgc tttccggatc tcaatgcgct gctcggccgg cagacgacgg
    23101 cggccgaaca ggactttctt gccagccccg gaagttcctt tctctgaacc ggcgcgcgaa
    23161 atccccggcg cgtgcagggc tcctgcactt cgattgaccc agatcaatat ccggggctgg
    23221 cacggtggcg gaacaggatt aagctgttcg tcagtcaggc aaggaaatgt atggaggcac
    23281 tatcgaaggc cagattcgcg gtgatgctga acaggccctc atcctacggt gaatccggta
    23341 acgcccttca ctgttccgac cggcgcagac cgttgcagat acgagcctgg atcattccgg
    23401 atactgtttg ccatcaaaac cctgagcgcc cccgcttccc tgatccggaa cgggggcgcg
    23461 gtgtttttgg agcctgaccg gcgaatccgg atcaccggac ccgctggaat cgggttttcc
    23521 ggcccggacg gggtcgttct tcgaaaccgc ccggcccgct cccggaaatc cgctgcgtca
    23581 gccgcgcgtc gcgtcagatc gggaagaacg cgtggatcag gaagcgcgtc agccagccgg
    23641 cggtattggc atcggcgacc gcgcccttgc cggcgtaggt gatctgcgca tcggcgacct
    23701 ggccggaggc gatcgaattg tcggcggcaa tgtcatcggg ccggaccacg ccctgcacga
    23761 tcacgaattc gtcgccctgg ttcagcgtga ccttcttttc gccgcgaatc tgcaggttgc
    23821 cgttcggata aacctcggtg accaccgcgg tgatcgtgcc ggtgatgctg ttggactgat
    23881 cggcactgcc cgaaccctcg aaatcgcgtt cgctgctcag atcgatctcg gctttcggat
    23941 acaccttgcc gaacagcacc gccggtgccg cgaccgaggc attcgaggat ttgtcgagct
    24001 tggtatcggc cttcttcgag gcattggtgc gttcggcgag ccggatcgtg atcacgtcgc
    24061 cgatgcgccg ggccgtgcgc gattcgaaca ggcgcagatc ggtgccggcg cggtagatcg
    24121 aaccatcggc gacgcgcggg cgtgcctgcg gcaccgtcgt catcaccgtc ggcatgaccg
    24181 gcctgaattc gtgatcgagc ggcagcgtcg tgcaggcggt gctcagggcg gccacgagca
    24241 cgaggccgag gcgcagacag ggattcatcg cggcggacct cagacgttct ggttcaggta
    24301 ctgcagcatc tggtcggtgg tctggatggc cttggagttg acctcgtagg cgcgctgcgt
    24361 ctcgatcatg ctgaccagtt cctcggcgac gttgacgttc gagctctcca gcatgtgctg
    24421 ctgcagcgcc ccgagaccgt tggtgcgcgg atcaccgagc tgggctgcgc cgctcgcgcc
    24481 gctttcgagg aacaggttct cgccgatcgg ctgcaggccg gccggattca ggaagtcggc
    24541 gatctgcagg ttgccgatct gctgcggcgc gatctggccg gcgagcgtga cgctgaccac
    24601 gccgtcggaa cccaccgtca ggctctgcgc gccgttcggc accgtcaggt tcggcagcag
    24661 cggatagcct tcggaggtga cgatctggcc ctggtcgttg atctggaacg aaccgtcgcg
    24721 ggtataggcc gtcgtgccgt ccggcagctg gatctggaag aagccgcggc cgttgatggc
    24781 cacgtccagg gcgttgtcgg tgttgatgac gttgccctgc gtatgcatct tttcggtggc
    24841 caccacgcgc acgccggtac cgagcatcag gcccgagggc agcgtcgtgt tctgcgaggt
    24901 ctggccgccg acctgacgga cgttctggta cagcaggtcc tcgaacaccg cgcggctctt
    24961 cttgaagccg gtggtgttga cgttggcgag gttgttggcg atcacgtcca tgcgcgtctg
    25021 ctgggcatcg agcccggtct tggcgaccca gagggcggga ttcatggcat cggctcctgt
    25081 cagctcaggc tcatcaggcg gtcagtgctg cgcgcggcgt catcggccgc cgacatcagc
    25141 ttcacctggg tttcgtagcg ccgtgccagc tcgatcatcg agaccagcgc atgcgcggtg
    25201 ttgacgttgc tgccttcgag cgctccgggc acgagctgca ccgaggcatc cggcgcgaac
    25261 ggtcctccgg ccttgctgcg cagcagacca tcggcaccct tgatcaggtc attggccgcg
    25321 ggcctgacca gcttgatgcg atcgagcgcg acgacgttgg tctcgccgct cggcaggatg
    25381 ctgatcgtgc cgtcgccggc gatgtcgagc ttgtcgaccg gcgggatcac gatcggcccg
    25441 cgttcgccga gcagcacgtg accgtcggcc gtcatcagcc ggccgccgcc atcgatgcgc
    25501 aggttgccgg cgcgcgtgta ggcctcgccg ccgcggccgt cgtcgatcgc cagatagccg
    25561 tcggtcttca gcgccacgtc gagatcacga ccggtggttt cgatgccgcc ggccgtgaaa
    25621 tcgacgccgc tgcgctcggc catcgcatag acgcgcgtcg gcaggccgtc gccgatgacc
    25681 ggctggctgc gcatcgtggc caggtcctgc cggaagccgg tcgtcgcggc attggcgagg
    25741 ttgttgctga ccacggtctg cgcccgcagg atctgctgcg caccgttcat cgcgacgtaa
    25801 agcatgcggt ccatgggagg ctctccggac ggcgggacgg gcgggtggca agctggctat
    25861 cagcggatct gcagcagcgt ctgcgtcagc tggtcggccg tctggatcac ctgcgcgttc
    25921 gcctggaagt tgcgctgcgc ctggatcatg ttgacgagct g
//
LOCUS       DAOQIE010000014        24298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_905_length_24298_cov_8.011426, whole genome shotgun sequence.
ACCESSION   DAOQIE010000014 DAOQIE010000000
VERSION     DAOQIE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 24298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24298
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_24298_cov_8.011426"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /gene="aspS"
                     /locus_tag="PK144_01805"
     CDS             <1..1700
                     /gene="aspS"
                     /locus_tag="PK144_01805"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNF64488.1"
                     /translation="HRRRDHGGVIFVDLRDRDGLVQVVIDPDTAEAFAAAERVRSEYV
                     LRIRGRVRSRPEGTDNPNLASGKVEVLAKDLEILNRAETPPFQMDDEDISEEVRLRYR
                     YLDLRRPLMQERLKLRARTVSHFRRYLDANGFLDIETPMLTKATPEGARDYLVPSRTH
                     PGEFFALPQSPQLFKQLLMMAGFDRYYQIVRCFRDEDLRADRQPEFTQLDIETSFLDE
                     ADITGLMEGMIRGTFREILGVELPDPFPRMPFAEAMARFGSDKPDLRIPMELTELTDV
                     MKTEEFKVFAGAANQPRGRVAALKVPAGGEKLSRKQIDDYTGFVARYGAKGLAYIKVN
                     DAAKGREGLQSPIVKNLSDAALAAILERTGAVDGDLVFFGADKAKVVNDALGALRLKI
                     GQDLELIEGEWAPLWVVDFPMFEWDEDAGRWNAMHHPFTSPRDAVESVKADPENAVAR
                     AYDMVLNGTEIGGGSIRIHRPDMQSAVFELLGIDAEQAKLKFGFLLDALKFGCPPHGG
                     LAFGVDRLIMLMAGAKSIRDVMAFPKTQTAACLLTDAPAAVDEKQLRELNIRLRRTP"
     gene            complement(1803..2078)
                     /locus_tag="PK144_01810"
     CDS             complement(1803..2078)
                     /locus_tag="PK144_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNF64489.1"
                     /translation="MTANKPATPTGWIDPDDAPELTDAFFEQADEYVGDTLVRRGRPK
                     AASPKQALTIRYDADVVAAFKATGKGWQTRMNDALRQWLVEEQKRRA"
     gene            complement(2038..2310)
                     /locus_tag="PK144_01815"
     CDS             complement(2038..2310)
                     /locus_tag="PK144_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNF64490.1"
                     /translation="MKIEFDPGKRDKTLAERGLDFARADEVFAGRHFTAEDAREDYSE
                     PRYITVGKLDDRMVVMVWTPRGEARRIISLRKTNDREQARHAHRVD"
     assembly_gap    2501..2600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2750..6037
                     /locus_tag="PK144_01820"
     CDS             2750..6037
                     /locus_tag="PK144_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64491.1"
                     /translation="MTPSRRDALRIALIYAAFAAIWILFSDIAAEALAPDRAWLTRLS
                     MYKGWLFVGVTALLLYALIRSTLARVEAAEAERVRLYSELAAQLRTFDTVLSACPDLF
                     FLFDADGRCRYISRNGLDLLGLKAVAAHGRTLFELGLPPAVAASCETARLRALGSRDI
                     VTHEIDLPGAQGALCYEILHCALPDAAGRAGGTLATVRDLSARKHDERRIRQLTELYA
                     ALSETGRAVMRVRSRAELYAQVCRIATDHGGLCFAWIGTLDAAAACVRPRVFSGTAGD
                     YLHQLQVFPAGPDAVGPVAEAMRRGAPSVENDLLAMLGRLGLQRAAVLAGKYGLCSAG
                     VFPLREGGCISGVLTVYATEAGFFTPERIGLLAAMAEEIGHALDGQQLAEERARAQAR
                     LAESESRYRELFEASPQPMWVVEAGTLGFLAVNQAALDAYGHARERFLQMALPDLHAS
                     AGAPSGLPDAADRESGEPARCQGLARHRCADGRPIEVEWVAHALVWSAREAWLMLVTD
                     VTERRRAEAGLRLAAQVFEGSGEAIFIADEQQRIVSLNRACAAITGYPAAELPGQPLE
                     RLLGSDDDPDALAACLQALATGGAWKGELWSRRCDGSPYPQWLSMSAVRDPEGRTTHT
                     VAIFTDISERKAAEARIRHLAQHDFLTGLPNRRLLEDRLQQAVAFAARRSGRVGLLFL
                     DLDRFKIINDTCGHPVGDALLRELACRLTGVLRESDTLGRSGGDEFIVLLPGLDDPAD
                     AGRVGRKLLAAICEPVQVGEHELYVTGSVGIAVYPEDGHDAETLVRHADTAMFHAKHA
                     GRNQCQYFTPAMNTANDARIWLENALRRVLERGELRLEFQPQVEVASGRTLGAEALLR
                     WRHSERGEIAPADFIAVAEDCGLIVPIGAWVLESACRQAREWLDAGLPPVRLSVNVSA
                     VQFRSPGLGASVAAALAASGLEPGWLELEITEGCLMEDATAARTTLEALRAHGVSIAI
                     DDFGAGYSSLAYLKHFPIQRLKIDRSFVSGLPDDASDAAIARAIVHLAQSLRLETVAE
                     GVETAAQRDFLAGLGCESAQGWLWSRALDPEDFAARLLAEASGAAGGHDPDCACGHCL
                     SRSDHPL"
     gene            complement(6096..7517)
                     /gene="eat"
                     /locus_tag="PK144_01825"
     CDS             complement(6096..7517)
                     /gene="eat"
                     /locus_tag="PK144_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HNF64492.1"
                     /translation="MSSPTPRHPSSPASGPASLERTLGPWQLWGIAVGLVISGEYFGW
                     SYGWASAGTLGFLVTSTLVALMYACFIFSFTELTTAIPHAGGPFAYARRAFGPAGGYL
                     AGAATLVEFVFAPPAIAMAIGAYLNVQFPGLDARTAAVGAYVIFMTLNILGVQVAATF
                     ELFVTVIAIVELLVFMGVVAPGFSLANFTAGGWAGADSFTWGSVAGIVAAIPFAIWFF
                     LAIEGAAMAAEEAIEPTRTIPIAYIAGILTLLVLAYGVMLFAGATGDWTKLSNINDPL
                     PQAMKVIVGENSGWLHMLVWIGLFGLIASFHGIIMGYSRQIYAQARAGYLPRWLAQVH
                     PRFHTPHRAILAGGVVGIAAIFSDELVSFGGQTLTANIVTMSVFGAIVLYLVSMASLF
                     RLRRLEPALPRPFRAPLYPLLPGIAFALAALCLLTMIWFNPQIAALFAGLLVLGYLYF
                     RLTAGQRAAAHDDTLLQAVPVAD"
     gene            7823..8632
                     /locus_tag="PK144_01830"
     CDS             7823..8632
                     /locus_tag="PK144_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64493.1"
                     /translation="MTPAHALLALNAVAGREVAKFCRQYGRLFSSLVRPSLWLIVFAA
                     GFQNVFGVAIIPPYDTYIEYRVYIAPGLLGMVLLFNGMQSSLAMVYDREMGMMRLLLT
                     APLPRWWLLFCKLAAGTLLSVLQAYAFLLVAAAFGVEIPAGGWLAVLPALAAGGLMLG
                     ALGLLLSVYVKQLENFAGTMNFVIFPMFFISSALYPLWKLRESGAEIVWHIAQWNPFT
                     HAVELIRYALYGRFEPLACTVVLGSAALFFIAATLGYDPQRGLLKRSGRPG"
     gene            8913..9377
                     /locus_tag="PK144_01835"
     CDS             8913..9377
                     /locus_tag="PK144_01835"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNF64494.1"
                     /translation="MKFIRILTALFTTALLAFSSTSAHAWATEKDALALLEQAVAEIK
                     DKGVDAALAEFNNPKGKFVKGELYVFAFDMEGTYKANGQNPALVGKPARDLVDVAGNP
                     VVATMLDIAKNKGEGEVEYMWLNPKVNKLQRKMSHIKVVNGLIVGVGHYFVN"
     gene            9492..10532
                     /gene="pip"
                     /locus_tag="PK144_01840"
     CDS             9492..10532
                     /gene="pip"
                     /locus_tag="PK144_01840"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNF64495.1"
                     /translation="MDSCLFPPCEPYAVHALAVGEGHVLHVEECGTPDGLPVIFLHGG
                     PGSGCNPLQRRLFDPARWRIVLVDQRGAGRSLPAGCIEHNSTADLLGDLERIRFALGI
                     DRWVVAGGSWGATLGLLYAEAFPDSTLGLLLRGTFLARPRDIAWYYRADGVALRFPSA
                     WRAFVGHLDETERRDLLTSYYRRLTSSDPEVVARAATAWAGWDNAVATWTLPPDREPQ
                     LPPDPLRRIAQARIAAHYLHHACFIVPNRVLANAHRLDGIPGILLHGELDRVCPIVQA
                     EALGAVWPDAELVRLPKAGHLLAEPPMLAAWVEAAARLYEAVAPAADRGRPASLFTCF
                     ADVGEQVSIDAG"
     assembly_gap    10615..10714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10718..11179)
                     /locus_tag="PK144_01845"
     CDS             complement(10718..11179)
                     /locus_tag="PK144_01845"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNF64496.1"
                     /translation="MSIPNYIEWSDELSVGIQEIDEQHKYLVQLVNELNVHLLSHGSA
                     KETVHEALDKLIRYTETHFVVEESLMRILGYPDYDSHRQHHEDLIQMVLDFRQQLNVE
                     GRVSRTELMEFLHKWLTYHILKEDKQYSEHFLSRGAQRSWLKGSWLKKFWK"
     gene            complement(11368..12504)
                     /gene="pqqE"
                     /locus_tag="PK144_01850"
     CDS             complement(11368..12504)
                     /gene="pqqE"
                     /locus_tag="PK144_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNF64497.1"
                     /translation="MSAAGYVPPEVPSRPLWLLAELTYACPLQCPYCSNPLDYTAVRE
                     ELDTETWKRVLREARAMGAAQLGLSGGEPATRTDLEEIVAEARRLGYYTNLITSGLGL
                     DATRIERLHAAGLDHIQVSFQASTEELNNHLAGTDAFRHKLEIARAVKAAGYPMVLCF
                     VLHRDNTDSMDAILTLAAQLGADYVELATTQYYGWAWVNRDRLLPTREQLVRAEAVAH
                     RWQADMKGRMKIYYVVPDYFEDRPKACMNGWGKVFLTVAPDGTALPCHSARELPGLSF
                     PNVRDHSVEWIWKDSPGFNRFRGYGWMKEPCRSCDEKEKDFGGCRCQAFLLTGDPENA
                     DPVCSKSPEHGRVLAAIEQAAATPSERPLVFRNPRSVRRLHGEA"
     gene            complement(12476..12739)
                     /gene="pqqD"
                     /locus_tag="PK144_01855"
     CDS             complement(12476..12739)
                     /gene="pqqD"
                     /locus_tag="PK144_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNF64498.1"
                     /translation="MSGARLRIAPGYRLQWEPAQSCHVLLYPEGMVQLNPSAAAVLEL
                     CDGSLDADGIVAALEAKYPGADLAADVRQFLEVADERGWLRSA"
     gene            complement(12736..13479)
                     /gene="pqqC"
                     /locus_tag="PK144_01860"
     CDS             complement(12736..13479)
                     /gene="pqqC"
                     /locus_tag="PK144_01860"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNF64499.1"
                     /translation="MSTQAPAWSREEFEARLRGLGSLYHIHHPYHVRMHEGQLSREQI
                     QGWVANRYYYQISIPRKDAAILANCPDRDTRRRWIQRIVDHDGNDAQEGGIEAWTRLG
                     IACGLDRETIESQRLVLPGVRFAVDAYVNFARSASWQEAACSSLTELFAPTIHRQRLN
                     HWPDRYPWIEPEGLQYFRNRLTEAHRDVQHGLAITLEHFASRLQQERALEILRFKLEV
                     LWTMCDAMWMAYVAELPPFTGCEAPAVPA"
     gene            complement(13476..14387)
                     /gene="pqqB"
                     /locus_tag="PK144_01865"
     CDS             complement(13476..14387)
                     /gene="pqqB"
                     /locus_tag="PK144_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNF64500.1"
                     /translation="MHIHVLGAGAGGGFPQWNCNCANCAGYRAGTLRATARTQSSIAV
                     SADGTDWILFNASPDIRAQLAAFAPMQPNRGIRDTGICAVLLIDSQIDHATGLLMLRE
                     HTRRLPVYLSAPAQQDLSTGLPLLKVLEHYCGTEVHELPLDGAAFTIPGAAGLVIRTV
                     ALASKAPPYSPHRHDPHPGDNVGVFIEDTRSGGRLFYAPGLGEIEDHLLPYLEQADLL
                     LVDGTFWTEDEMIRHGIVHKFAREMGHLPQSGEDGMIRALAPYRGRKVLIHINNTNPI
                     LVEDGPERAELAAHGIEVAYDGMDFEL"
     gene            complement(14537..14611)
                     /gene="pqqA"
                     /locus_tag="PK144_01870"
     CDS             complement(14537..14611)
                     /gene="pqqA"
                     /locus_tag="PK144_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNF64501.1"
                     /translation="MQWSKPEFTEMRFGFEVTMYIANR"
     gene            complement(15225..17285)
                     /locus_tag="PK144_01875"
     CDS             complement(15225..17285)
                     /locus_tag="PK144_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007990585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNF64502.1"
                     /translation="MLDRLASQHAARVLSVARDGDARAGRSAPDAAISRSWERCIANH
                     GLEPILPRGTLVLDRPALAQRQEKLQDFVELARVEMDRLYQQIAGSGSAIILTDADGV
                     ILNCVTSPGLARMFQRAGLWLGADWSERHEGTNGIGTCVMERQPVTVHRDEHFLARHI
                     GLSCSAATIFDPAGNLLAVLDASTVNSRDSKQSQFHALALAAMSAKTIENCAFQRHFR
                     QASVLRFHARPEYIGVIAEGLLAFDGDGRVLGANQSALNQLGGLSREELMHLRVADIF
                     DLSLDVLMGRASVHATVWPLLDRRGHGYYAMLRGPESALERGAMSMPAARRSTAAARP
                     AIPPQTAPAPGRTMSAVIAPRMDLEGLAGEDPAMAYNVRCARKVVDRNVTILLNGETG
                     TGKEAFAKAVHDASDRADKPFVAINCASIPENLIESELFGYKAGAFTGARREGMRGKI
                     VQAHGGTLFLDEIGDMPAHLQTRLLRVLEEREVVPLGGEAPIMVDLHVISATHRNLPD
                     LVASGSFREDLYYRLNGLVLTLPALRERADLAIVIERVLAAEAEGQPVELSFEARNLL
                     LEYPWPGNIRQLRNILRTAVALCEDAEIRPEDLPAELGRAPGMAGPGAAEAAPRLVSP
                     PVETIAATDPEGSLASAEKAALLRELERHRWNMTNTARDLGLSRNTLYRKLKKHGITP
                     PTLS"
     gene            17660..18091
                     /gene="pedF"
                     /locus_tag="PK144_01880"
     CDS             17660..18091
                     /gene="pedF"
                     /locus_tag="PK144_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNF64503.1"
                     /translation="MMKITMRAGLVLATLLTAATAHAHGDVTPQAVDTTGLDPLGDEN
                     WRESNPYRGNELAIKIGKSAFAQNCARCHGIDAISGGIAPDLRKLEKGALGDEWFQER
                     VRNGAVRNGAVYMPKFAKPEGALSQEALWAIRSWLDTVAID"
     gene            18317..19060
                     /locus_tag="PK144_01885"
     CDS             18317..19060
                     /locus_tag="PK144_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNF64504.1"
                     /translation="MAGHSKWANIQHRKGAQDAKRGKLFTRFIREITIAARSGLPDPA
                     ANPRLRLAVDKALGANMSKDTIERAIKRGAGADDGSTLEEIRYEGYGPGGIAVMVDCM
                     TDNRNRTVAEVRHAFSKCGGNLGTSGSVAFQFTETGVISYPAGTDQEKVMEVALEAGA
                     DDLVENEDGSFDVLTAPDAFAAVREALDGAGLVAESAEVTMRAGNTVALGLDDARSMI
                     KLLDMLEDLDDTQNVYSNADIPEAVMAQL"
     gene            19078..19578
                     /gene="ruvC"
                     /locus_tag="PK144_01890"
     CDS             19078..19578
                     /gene="ruvC"
                     /locus_tag="PK144_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNF64505.1"
                     /translation="MRILGVDPGSRLTGYGVIDVHGPRAVYVDSGCIRTPAGALGERL
                     CAIHRGLAEILRVHAPAAAAIEQVFLHRNAVSALKLGHARGVALLALAEAGLAVAEYM
                     PSVVKLAVVGTGRADKEQVQHMVTALLKLPRAPQADAADALAVAICHGTQLFAQARLA
                     GAGQGR"
     gene            19575..20189
                     /gene="ruvA"
                     /locus_tag="PK144_01895"
     CDS             19575..20189
                     /gene="ruvA"
                     /locus_tag="PK144_01895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNF64506.1"
                     /translation="MIGRLRGRLILRQPPLLMIELGNGLAYELEASLSTCCALPETGA
                     EVVLYTHLAVREDAQVLYGFGTLAERTLFRNLIRVTGIGAKLALVILSGMSVDAFARC
                     LEAGDVATLIRLPGIGRKTAERLVVEMRDRIQSLELGAEFASRLPARAPDAPPDPVQD
                     AEAALVALGYKPADATRMVRAVAADGLPSEELIRRALAATLSRG"
     gene            complement(20207..20449)
                     /locus_tag="PK144_01900"
     CDS             complement(20207..20449)
                     /locus_tag="PK144_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNF64507.1"
                     /translation="MDTPAAAETVLDLTGLLCPLPLLKTKQAMAKMESGERIRILATD
                     RSVVVDFRVFIDRFGHELLEFTESSGGHYVFVLRKA"
     gene            20900..21931
                     /gene="ruvB"
                     /locus_tag="PK144_01905"
     CDS             20900..21931
                     /gene="ruvB"
                     /locus_tag="PK144_01905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNF64508.1"
                     /translation="MIESDRLISAAASADDAAIDRAIRPKRLADYVGQQALREQLEIF
                     IHAARRRREALDHVLLFGPPGLGKTTLAHIIAAEMGVNLRSTSGPVLEKPGDLAALLT
                     NLEPYDLLFVDEIHRLSPVVEEVLYPAMEDYQIDILIGEGPAARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVHRLEFYTTAELTQIVGRSAGILGVPTDDDGAGEIGRRSRG
                     TPRIANRLLRRVRDYAEVRAGGHITQDIARAALAMLKVDEQGFDDMDRRLLGLLLDKF
                     GGGPVGLDTLAAALGEERGTIEDVLEPFLIQQGFLMRTPRGRTATAHAYRHFGLLPPG
                     GADLFDAPV"
     gene            21940..23379
                     /locus_tag="PK144_01910"
     CDS             21940..23379
                     /locus_tag="PK144_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64509.1"
                     /translation="MAVTRHGGRRWPGSLRSLIIAGFLLALTPPLAALGWAAYTLNSL
                     VAEVDAGLETAVAAARYGRGLVADVTELQRSARHWNVLQSDTLLDTYRERRKRVDETL
                     GQLRALALDPERVQRLERLAAALPVGDGPPVGDDDVLTAQLAPLVAQAVDVLELSDDA
                     VEGESARLREVTAEANRRFASLAALGLIAGSGCALLAGGLIARPTRQLAQAIRRLGDE
                     DFTTPVSLAGPRDIQLIGARLDWLRSRLGEIDAQKQRFLRHVSHELKTPLTALREGSE
                     LLRDGTLGPLAEDQREVAGILVDNARRLSTLIEDLLNFNRLLARTQQLEAGTVALAAL
                     VEEVAGAQRLAAQARCVTLELDVPEGLIVQADHDKLRTILDNLLSNALKFARRDGRIA
                     LRAGRYPGGVAVEVEDDGPGIPAEERERVFELFFQGSHQPDTPVRGSGLGLALAREFA
                     RLHGGDLIVADGRGSGACLCLTLPATAPP"
     gene            23495..23836
                     /locus_tag="PK144_01915"
     CDS             23495..23836
                     /locus_tag="PK144_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64510.1"
                     /translation="MRRHSPPLLLCLLAVLGTGCDTLNGMTDKPPSGDEAILRKQLVD
                     ARSVADRARASARKAEARATDLGKDKADLETTVAELRAQNERLTQENLDLKRRLQALG
                     RIESTLSDQGK"
     gene            23838..>24298
                     /locus_tag="PK144_01920"
     CDS             23838..>24298
                     /locus_tag="PK144_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64511.1"
                     /translation="MTDKRCDILAVDDDPDLLRLLVLRLKAAGYVVETATNGQEALTQ
                     LTLCAPRLLITDLRMPGMDGLALFDAVRSQRPGLPVIMLTAHGTIPDAIQATRDGVFG
                     FLPKPFEPAELLAEVQRALATTAPARGNPAGWRAAIVTRSPRLESVLDEAQL"
BASE COUNT         3536 a         8319 c         8529 g         3714 t
ORIGIN      
        1 cgcaccgccg ccgcgaccac ggcggcgtga tcttcgtcga cctgcgcgac cgcgacggtc
       61 tggtgcaggt cgtcatcgac ccggacaccg ccgaagcctt cgccgccgcc gagcgcgtgc
      121 gctccgaata cgtgctgcgc atccgcggcc gggtgcgcag ccgtcccgag ggcaccgaca
      181 acccgaacct cgcctccggc aaggtcgagg tgctcgcgaa ggatctcgaa atcctcaacc
      241 gcgccgagac cccgccgttc cagatggacg acgaggacat ctccgaggaa gtgcgcctgc
      301 gctaccgcta cctcgacctg cgccggccac tgatgcagga gcgcctgaag ctgcgtgcgc
      361 gcaccgtcag ccacttccgc cgctatctcg acgccaacgg cttcctcgac atcgagacgc
      421 cgatgctgac caaggcgacg cccgagggcg cgcgcgatta cctggtgccg tcgcgcacgc
      481 atccgggcga gttcttcgcg ctgccgcagt cgccgcagct gttcaagcag ctgctgatga
      541 tggccggctt cgaccgttac tatcagatcg tgcgctgctt ccgcgacgag gacctgcgcg
      601 ccgaccgcca gcccgaattc acgcagctcg acatcgagac ctccttcctc gacgaggccg
      661 acatcaccgg tctgatggaa ggcatgatcc gcggcacctt ccgcgagatc ctcggcgtcg
      721 agctgcccga tccgttcccg cgcatgccgt ttgccgaggc gatggcgcgc ttcggctccg
      781 acaagcccga cctgcgcatc ccgatggagc tgaccgaact caccgatgtc atgaagaccg
      841 aggaattcaa ggtcttcgcc ggggcggcca accagccgcg cgggcgcgtc gcggcgctga
      901 aggttccggc cggtggcgag aagctctcgc gcaagcagat cgacgactac accggcttcg
      961 tcgcccgcta cggcgccaaa ggcctcgcct acatcaaggt caatgacgcc gcgaagggcc
     1021 gcgagggcct gcagtcgccg atcgtcaaga acctctccga cgccgccctc gccgcgatcc
     1081 tcgaacgcac cggcgccgtc gacggcgatc tggtgttctt cggcgccgac aaggcgaagg
     1141 tcgtcaacga cgccctcggc gcgctgcgcc tgaagatcgg tcaggatctc gagctgatcg
     1201 aaggcgaatg ggcaccgctg tgggtcgtcg atttcccgat gttcgaatgg gacgaggacg
     1261 ccggtcgctg gaacgcgatg catcacccgt tcacgtcgcc gcgcgatgcg gtcgaatcgg
     1321 tgaaggccga cccggaaaac gccgtcgccc gcgcctacga catggtgctg aacggcaccg
     1381 agatcggcgg cggctcgatc cgcatccacc gcccggacat gcagagcgcc gtgttcgagc
     1441 tgctcggcat cgacgccgaa caggcgaagc tcaagttcgg cttcctgctc gatgcgctga
     1501 agttcggctg cccgccgcac ggcggtctcg ccttcggcgt cgaccggctg atcatgctga
     1561 tggccggtgc gaagtcgatc cgcgatgtca tggccttccc gaagacccag accgccgcct
     1621 gcctgctgac cgatgcgccg gccgcggtcg acgagaaaca gctgcgcgag ctgaacatcc
     1681 ggctgcggcg cacgccgtga gccggcgctg atcggcgcgg gatgaaaaac ggggagcctg
     1741 cgggctccct ttgttttcgg gcatgcacct ttgactgcgt gtgcaacgca ggcaacaccc
     1801 agtcaggcgc gccgcttctg ttcctcgacc agccactggc ggagggcatc gttcatgcgc
     1861 gtctgccagc ctttgccggt cgccttgaag gctgccacca cgtcggcatc gtagcggatc
     1921 gtcagggcct gcttcgggct ggccgccttc gggcggcccc ggcgcaccag cgtgtcaccg
     1981 acgtactcgt cggcctgctc gaagaatgca tcggtcagtt ccggcgcatc gtccgggtca
     2041 atccacccgg tgggcgtggc gggcttgttc gcggtcattg gtttttctca ggctgatgat
     2101 gcggcgagcc tcgccgcgcg gggtccagac catcaccacc atccggtcat ccagcttgcc
     2161 gacggtgatg tagcgcggct cgctgtagtc ctcacgggcg tcctcggcgg taaagtgccg
     2221 gcccgcaaac acctcgtcag cgcgggcgaa gtccagtcct cgttcggcga gggtcttgtc
     2281 gcgctttccg gggtcgaact caattttcat ggtatttact gtagttacat aaaatccgca
     2341 cgcaagaatt ttttgtaact acaccatggc aaggcacagc cctcccggcg agcaacggcc
     2401 cgtccctcgc gcacggcgtc cacgattgcc gaggtctcac ccgctcccga gcagaaagtt
     2461 gcgccccgcg cccgcgccgc ccggcagccg cgcaatctcg nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn ggcgctgcgc gccggccggt cgagccggat gtcgcaagct
     2641 gtggcacctg cgtatgcggc aatgcggctt ggatagactg gccgcgtccg ccgatcggca
     2701 cggttccgtg gcgggccgat cgctcccgag ccgccaccgg tctgcgccga tgactccgtc
     2761 ccgccgcgat gcgctgcgca tcgcgctgat ctatgcggcc ttcgccgcca tctggatcct
     2821 gttctccgac atcgccgccg aggcgctggc gccggaccgg gcgtggctga cgcggctgtc
     2881 gatgtacaag ggctggctgt tcgtcggggt cacggcgctg ctgctgtacg cgctgatccg
     2941 cagcacgctc gcgcgcgtcg aggctgccga ggccgagcgc gtgcggctgt attcggaact
     3001 cgccgcgcag ctgcgcacct tcgacaccgt gctgtcggcc tgtccggacc tgttcttcct
     3061 gttcgatgcc gacgggcgct gccgctacat cagccgcaac ggcctcgacc tgctcggact
     3121 gaaggccgtc gccgcgcacg ggcgcacgct gttcgagctc ggcctgccgc cggcggtcgc
     3181 ggccagctgc gagacggcac gcctgcgcgc gctcggcagc cgcgacatcg tcacgcacga
     3241 gatcgatctg ccgggtgcgc agggagcgct ctgctacgag atcctgcact gcgcgctgcc
     3301 cgatgccgcc gggcgcgccg gcggcacgct ggcgacggtg cgcgacctga gcgcgcgcaa
     3361 gcacgacgag cgccgcatcc gccagctcac cgagctgtac gcggcgctga gcgagaccgg
     3421 ccgcgccgtg atgcgcgtgc gcagccgggc cgagctgtac gcgcaggtct gccgcatcgc
     3481 caccgaccac ggcggactct gcttcgcgtg gatcggcacg ctcgatgccg cggccgcctg
     3541 cgtgcgcccg cgggtgttca gcggcacggc cggcgattac ctgcatcagc tgcaggtctt
     3601 cccggccggt ccggacgccg tcggtccggt cgccgaagcc atgcgccgcg gagcaccgag
     3661 cgtcgagaac gatctgctgg cgatgctcgg acgcctcggc ctgcagcgtg cggccgtgct
     3721 cgccggcaag tacgggctgt gctcggccgg cgtgttcccg ctgcgcgagg gtggatgcat
     3781 cagcggcgtg ctgaccgtct acgccaccga ggccggcttc ttcacgcccg agcgcatcgg
     3841 cctgctggcg gcgatggccg aggagatcgg ccacgcgctc gatggccagc agctcgccga
     3901 ggagcgcgcg cgcgcgcagg cgcggctggc cgagagcgag agccgctacc gcgagctgtt
     3961 cgaggccagc ccgcagccga tgtgggtggt cgaggccggc acgctcggtt ttctcgccgt
     4021 caaccaggcg gcgctcgatg cctacggcca tgcgcgcgag cgcttcctgc agatggcgct
     4081 gcccgatctg cacgcaagtg ccggggcgcc gtccggcctg ccggatgccg cggatcgcga
     4141 gtccggggag ccggcgcgct gccaggggct cgcgcggcac cggtgcgccg acggccggcc
     4201 gatcgaggtc gagtgggtcg cgcacgcgct ggtctggagc gcacgcgagg cctggctgat
     4261 gctcgtgacc gacgtgaccg agcgccggcg cgccgaggcc gggctgcggc tggccgcgca
     4321 ggtgttcgag ggcagcggcg aggcgatctt catcgccgac gaacagcagc gcatcgtgtc
     4381 gctgaaccgc gcctgcgccg ccatcaccgg ctacccggcg gcggaactgc ccggccagcc
     4441 gctcgaacgc ctgctcggca gcgacgacga tccggacgcc ctggctgcct gcctgcaggc
     4501 gctcgccacc ggcggggcct ggaagggcga gctctggagc cgccgctgcg acggctcgcc
     4561 gtacccgcag tggctgtcga tgtcggcggt gcgcgatccg gagggccgga ccacgcatac
     4621 cgtcgcgatc ttcaccgaca tcagcgagcg caaggccgcc gaggcgcgca tccgccacct
     4681 cgcgcagcat gacttcctga ccgggctgcc gaaccgccgg ctgctcgaag accggctgca
     4741 gcaggccgtg gcgttcgcgg cacggcgcag cggacgggtg gggctgctgt tcctcgatct
     4801 cgaccgcttc aagatcatca acgacacctg cggccacccg gtcggcgatg cgctgctgcg
     4861 cgaactcgcc tgccggctga ccggggtatt gcgcgaatcc gacaccctcg ggcgttcggg
     4921 gggcgatgag ttcatcgtgc tgctgcccgg actcgatgac ccggccgatg ccgggcgcgt
     4981 cgggcgcaag ctgctggcgg cgatctgcga accggtgcag gtcggcgagc acgagctgta
     5041 cgtgaccggc agcgtcggca tcgccgtgta tccggaggac ggtcacgacg ccgaaaccct
     5101 ggtgcgccac gccgacacgg cgatgtttca tgccaagcac gccggtcgca accagtgcca
     5161 gtacttcacg ccggcgatga acaccgccaa cgatgcgcgc atctggctcg agaacgcgct
     5221 gcgccgcgtg ctcgaacgcg gcgagctgcg cctcgaattc cagccgcagg tcgaggtggc
     5281 gagcgggcgc acgctcggtg ccgaggcgct gctgcgctgg cgtcactccg agcgcggcga
     5341 gatcgcgccg gcggacttca tcgcggtggc cgaggactgc ggactgatcg tgccgatcgg
     5401 cgcctgggtg ctggaatcgg cctgccggca ggcgcgggaa tggctcgatg ccggcctgcc
     5461 gccggtgcgg ctgtcggtga acgtctcggc cgtgcagttc cgctccccgg gcctgggcgc
     5521 gagcgtcgcc gcggcgctgg cggcgagcgg gctggagccg ggctggctgg agctggagat
     5581 caccgaaggc tgcctgatgg aggacgccac ggccgcgcgc acgacgctgg aggcgctgcg
     5641 cgcgcacggg gtcagcatcg ccatcgacga cttcggcgcc ggctattcga gccttgccta
     5701 cctgaagcac ttcccgatcc agcgcctgaa gatcgaccgt tccttcgtca gcggcctgcc
     5761 cgacgatgcc agcgacgcgg cgatcgcgcg cgcgatcgtg catctggcgc agagcctgcg
     5821 cctcgaaacc gtcgccgaag gcgtcgagac cgccgcgcag cgcgacttcc tcgccggact
     5881 cggctgcgag tccgcccagg gctggctgtg gtcacgcgcg ctcgaccccg aggacttcgc
     5941 cgcccggctg ctggccgagg ccagcggggc cgccggtggc cacgacccgg actgcgcctg
     6001 cgggcactgc ctgagccgca gcgatcatcc gctctgaggc cggacggcgg ccgcgagcgc
     6061 gccccgggcg cgttcgcccg gtcgcgccgc acgcgctagt cggccaccgg cacggcctgc
     6121 agcagcgtgt cgtcgtgcgc cgcggcgcgc tggccggcgg tcagccggaa gtacagatag
     6181 ccgagcacga gcaggccggc gaacagcgcg gcgatctgcg ggttgaacca gatcatcgtc
     6241 agcaggcaga gcgcggcgag cgcgaaggcg atgcccggca gcagcgggta cagcggcgcg
     6301 cggaacggcc gcggcagcgc gggctcgagc cggcgcagcc ggaacaggct ggccatgctg
     6361 accagataca gcacgatcgc gccgaacacc gacatcgtga cgatgttggc ggtcagcgtc
     6421 tggccgccga agctgaccag ttcgtcggag aagatcgcgg cgatgccgac cacgccgccg
     6481 gcgaggatcg cgcggtgcgg ggtgtggaag cgcgggtgca cctgcgcgag ccagcgcggc
     6541 aggtagccgg cgcgcgcctg cgcgtagatc tggcgcgagt agcccatgat gatgccgtgg
     6601 aagctcgcga tcagcccgaa caggccgatc cacaccagca tgtgcagcca gcccgagttt
     6661 tcgccgacga tcaccttcat cgcctgcggc agcggatcgt tgatgttcga gagcttcgtc
     6721 cagtccccgg tcgcgccggc gaacagcatg acgccgtagg cgagcacgag cagcgtcagg
     6781 atgccggcga tgtaggcgat cgggatcgtg cgcgtcggct cgatggcctc ctcggcggcc
     6841 atcgccgcgc cctcgatcgc gaggaagaac cagatcgcga acgggatcgc cgcgacgatg
     6901 ccggcgaccg agccccaggt gaagctgtcg gccccggccc agccgccggc cgtgaagttc
     6961 gcgagcgaga agcccggcgc gaccacgccc atgaacacca gcagctcgac gatcgcgatc
     7021 acggtcacga acagctcgaa ggtcgccgcg acctgcacgc cgaggatgtt cagcgtcatg
     7081 aagatcacgt aggccccgac cgcggcggtc ctggcatcga gccccgggaa ctgcacgttc
     7141 aggtaggcgc cgatcgccat cgcgatcgcc ggcggggcga acacgaactc gaccagcgtc
     7201 gccgcaccgg ccagataccc gccggccggg ccgaaggcgc ggcgcgcata cgcgaacggg
     7261 ccgccggcgt gcgggatcgc cgtggtcagc tcggtgaagc tgaagatgaa gcacgcgtac
     7321 atcagcgcga ccagcgtcga ggtgacgagg aagccgagcg tgccggcgct ggcccagccg
     7381 tagctccagc cgaagtattc accggagatc acgaggccga cggcgatgcc ccagagctgc
     7441 caggggccga gggtgcgctc cagcgacgcg gggccgctgg cgggggagga cggatgacgc
     7501 ggcgtggggc tcgacatgat gcggcagact cccgggggcg ggtggggaac gggatgcgcg
     7561 gcgtccgcag gcggttgcgg atgctgcgat gcagcgatac aagcccgatg ccatcggcgc
     7621 acacggcccc caagtcgctg atcggtcgtg tgcggcttgt tcatgtcggc cggccagcgt
     7681 caaggctcct gacagttgcg cccgccggcg gggcggctgc accggtgcgg gtcgtggcgc
     7741 aggcgccggg gttcgcgggc gtttccgcag gcgtttaagc tgcgcggcat cctttcccag
     7801 cgctgccgag gcccgtccgc cgatgacccc cgcccacgcc ctgctcgcgc tcaacgccgt
     7861 tgccggccgc gaagtcgcga agttctgccg ccagtacggg cggctgttct cgtcgctggt
     7921 gcgcccgagc ctgtggctga tcgtgttcgc cgccggtttc cagaacgtgt tcggcgtcgc
     7981 gatcatcccg ccctacgaca cctacatcga ataccgcgtc tacatcgcgc cgggcctgct
     8041 cggcatggtg ctgctgttca acggcatgca gtcgagcctg gcgatggtct acgaccgcga
     8101 gatgggcatg atgcggctgc tgctgaccgc gccgctgccg cgctggtggc tgctgttctg
     8161 caagctcgcg gccggcacgc tgctgtcggt gctgcaggcc tatgccttcc tgctggtggc
     8221 ggcggccttc ggcgtcgaga ttccggccgg cggctggctg gcggtgctgc cggcgctcgc
     8281 ggccggcggg ctgatgctcg gcgcgctcgg gctgctgctg tcggtgtacg tgaagcagct
     8341 cgaaaacttc gccggcacga tgaatttcgt gatcttcccg atgttcttca tttcctcggc
     8401 gctgtacccg ctgtggaagc tgcgcgagtc gggcgccgag atcgtctggc acatcgccca
     8461 gtggaacccg ttcacgcatg cggtcgagct gatccgctac gcgctgtacg ggcgcttcga
     8521 gccactggcc tgcacagtcg tgctcggcag cgcggcgctg ttcttcatcg cggctacgct
     8581 gggctacgac ccgcagcggg ggctgctgaa gcgcagcggc cggccgggct gaggccgcga
     8641 tacgccagac ggcgcgcagc ctgtgccgga tggcgtaccg attccgtgac agggcggctg
     8701 tgacgccgcg cggacagctg tttcgccccg gtcgcgggcc atgtcaaatc agcgtcttgc
     8761 tgtcaatggc ttgctccttg ctatgggtca gtgcagtcgc tggggcgccg cgcgaggctt
     8821 gcagacgcgg cgttccgcgc acccttccgt tgcccgtcag cggcacattc caaacaagct
     8881 tcgacaagct tcagaccgga ggagaaagct ccatgaaatt catcaggatc ctgacggccc
     8941 tgttcacgac cgcgctgctg gccttcagct cgaccagcgc ccacgcctgg gcgaccgaga
     9001 aggacgcgct ggcgctgctg gaacaggccg tcgccgagat caaggacaag ggtgtcgatg
     9061 ccgcgctcgc cgagttcaac aatccgaagg gcaagttcgt gaagggcgag ctgtacgtgt
     9121 tcgcgttcga catggagggc acctacaagg ccaacggcca gaatcccgct ctggtcggca
     9181 agccggcccg tgacctcgtc gatgtcgccg gcaacccggt ggtcgcgacc atgctcgaca
     9241 tcgccaagaa caagggcgag ggcgaggtcg agtacatgtg gctgaacccg aaggtcaaca
     9301 agctgcagcg caagatgtcg cacatcaagg tcgtcaacgg cctgatcgtc ggcgtcggtc
     9361 actatttcgt gaactgagcc gtgaactgat ccgtgagcgg agcggccttc ccgccgctgc
     9421 gtcgtcacga cgggactttc tgcaggcaca cgccggccgg ctgcggccgg cgtgcggccg
     9481 gagggcgaac cttggacagc tgcctgtttc cgccctgcga gccgtacgcg gtgcatgccc
     9541 tcgccgtcgg cgagggccat gtgctgcacg tggaggaatg cggcacgccc gacgggctgc
     9601 cggtgatctt cctgcacggt ggcccgggtt cgggctgcaa tccgctgcag cggcggctgt
     9661 tcgatccggc gcgctggcgc atcgtgctgg tcgatcagcg cggggccggg cgttcgctgc
     9721 cggccggctg catcgaacac aattcgaccg ccgacctgct cggcgatctc gaacgcatcc
     9781 gctttgcgct cggcatcgac cgctgggtcg tcgccggcgg ctcctggggg gcgacgctcg
     9841 gcctgctgta cgccgaggcc tttccggaca gcacgctcgg gctgctgctg cgcggcacct
     9901 tcctcgcgcg cccgcgcgac atcgcctggt attaccgcgc cgatggcgtg gcgctgcgct
     9961 ttccgagcgc ctggcgggcc ttcgtcggcc atctcgacga gaccgagcgc cgtgacctgc
    10021 tgaccagtta ctaccggcgc ctgacctcgt cggacccgga ggtcgtcgcg cgggccgcca
    10081 ccgcctgggc gggctgggac aacgccgtcg cgacctggac gctgccgccc gatcgcgagc
    10141 cgcagctgcc acccgatccg ctgcggcgca tcgcgcaggc gcgcatcgcc gcgcactacc
    10201 tgcatcacgc ctgcttcatc gtgccgaacc gggtgctcgc caacgcccat cgtctcgacg
    10261 gcatccccgg catcctgctg cacggcgagc tcgatcgcgt ctgcccgatc gtgcaggccg
    10321 aggcgctcgg ggcggtatgg ccggatgcgg agctggtgcg cctgccgaag gccgggcatc
    10381 tgctcgccga gccgccgatg ctggcggcct gggtcgaggc ggccgcacgg ctgtacgagg
    10441 ccgtggcacc ggcggctgat cgcggccggc cggcctcgct gttcacctgc ttcgccgatg
    10501 tcggcgagca ggtcagcatc gacgccggct gaaacgcaac gacgccggca ggccggcgtc
    10561 gtgttcagcg ggttccggac agcgcgcccg gtgcgggccc tcttccgggc ttcannnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcttca cttccagaac ttcttcagcc
    10741 acgagccctt cagccacgag cgctgcgcgc cacggctcag gaagtgctcc gagtactgct
    10801 tgtcttcctt caggatgtga taggtcagcc acttgtgcag gaactccatg agttcggtgc
    10861 gcgagacgcg accttccacg ttgagctgct ggcggaagtc gagcaccatc tggatcaggt
    10921 cctcgtggtg ctggcggtgc gagtcgtagt cgggatagcc gaggatgcgc atcaggcttt
    10981 cctcgaccac gaagtgcgtc tcggtgtagc ggatcagctt gtccagggct tcgtgcacgg
    11041 tctccttcgc ggagccgtgg ctcagcaggt gcacgttcag ctcattgacc agctgcacca
    11101 gatacttgtg ctgctcgtcg atctcctgga tgccgacgga aagctcgtcg ctccattcga
    11161 tgtaattcgg aatgctcatg tggccgactc tctccggtgg tcgcttgatg gtccggtgcc
    11221 gcggcgcgcg gttcggaaga tgcgaaacgt agccggcacc ggacctcggc gggctgatgc
    11281 aggtcagccg ccccggggcg gctgatccgt cggacaccga gtattacatt cagtttattt
    11341 ttcgccagtg gcgagggcgc cggacgctca ggcttcgccg tgcaggcgac ggacgctgcg
    11401 cggattgcgg aacaccagcg gccgttcgga cggcgtcgcc gcggcctgtt cgatcgcggc
    11461 cagcacgcgg ccgtgctcgg gcgacttgct gcagaccgga tcggcgttct cggggtcgcc
    11521 ggtcagcagg aaggcctggc agcggcagcc gccgaagtcc ttttccttct cgtcgcagga
    11581 gcggcagggt tccttcatcc agccgtagcc gcggaagcgg ttgaagccgg gggagtcctt
    11641 ccagatccat tcgaccgagt gatcgcgcac gttcgggaag ctcaggcccg gcagctcgcg
    11701 cgccgagtga cagggcagtg cggtgccatc gggggcgacg gtcaggaaca ccttgcccca
    11761 gccgttcatg caggccttcg gccggtcctc gaaatagtcg ggcacgacgt agtagatctt
    11821 catgcggccc ttcatgtcgg cctgccagcg gtgcgcgacc gcttcggcgc gcacgagctg
    11881 ctcgcgcgtc ggcagcagcc ggtcgcggtt gacccaggcc cagccgtagt actgcgtggt
    11941 cgcgagctcg acgtagtcgg caccgagctg tgccgccagc gtcaggatcg cgtccatcga
    12001 gtcggtgttg tcgcggtgca gcacgaagca gagcaccatc gggtagccgg cggccttgac
    12061 cgcgcgcgcg atctccagct tgtgccggaa ggcatcggtg ccggcgaggt ggttgttgag
    12121 ctcctcggtg ctcgcctgga agctcacctg gatgtgatcg agtccggccg cgtgcaggcg
    12181 ctcgatgcgt gtcgcgtcga ggccgagtcc ggaggtgatc aggttcgtgt agtaaccgag
    12241 ccggcgcgcc tcggcgacga tctcctcgag gtcggtgcgc gtcgccggct cgccgccgga
    12301 aagcccgagc tgggccgccc ccatcgcccg ggcctcgcgc agcacgcgct tccaggtctc
    12361 ggtgtcgagc tcctcgcgca cggccgtgta gtcgagcggg ttcgagcagt acgggcactg
    12421 cagcggacag gcgtaggtca gctcggcgag cagccacagc ggccgtgacg gtacttcagg
    12481 cggaacgtaa ccagccgcgc tcatcggcga cctccaggaa ctggcgcacg tcggcggcga
    12541 gatcggcgcc gggatacttc gcttcgaggg ccgcgacgat gccgtcggca tcgaggctgc
    12601 cgtcacagag ttcgagcacg gcagcggcgg aagggttgag ctgcaccatg ccttccggat
    12661 acagcagcac gtgacagctt tgcgccggct cccactgcag gcggtagccg ggcgcgatgc
    12721 gaaggcgcgc gccgctcatg ccggaaccgc cggggcttcg cagccggtga acggcggcag
    12781 ctcggcgacg taggccatcc acatcgcatc gcacatcgtc cacagcactt cgagcttgaa
    12841 gcgcaggatc tcgagcgcgc gttcctgctg gaggcggctc gcgaagtgct ccagcgtgat
    12901 cgcgaggccg tgctgcacgt cgcggtgcgc ctcggtcagg cggttgcgga agtactgcag
    12961 gccctcgggc tcgatccacg ggtagcggtc cggccagtgg ttcaggcgct ggcggtggat
    13021 ggtcggcgcg aacagttcgg tcagcgacga gcaggcggcc tcctgccagc tcgccgaacg
    13081 cgcgaagttc acgtaggcat cgaccgcgaa gcgcacgccc ggcagcacca ggcgctgcga
    13141 ctcgatggtc tcgcgatcga gaccgcaggc gatgccgagc cgcgtccagg cctcgatgcc
    13201 gccttcctgc gcgtcgttgc cgtcgtgatc gacgatgcgc tggatccagc gccggcgcgt
    13261 gtcgcggtcc gggcagttgg cgaggatcgc cgcatccttg cgcgggatgc tgatctggta
    13321 gtagtagcgg ttcgcgaccc agccctggat ctgctcgcgg ctcagctgcc cctcgtgcat
    13381 gcgcacgtgg taggggtggt ggatgtggta caggctgccg aggccgcgca ggcgcgcctc
    13441 gaattcctca cggctccagg ccggcgcttg cgtgctcata gttcgaaatc catcccgtcg
    13501 taagccactt cgatgccgtg cgcggccagt tcggcgcgct cgggcccgtc ctcgacgagg
    13561 atcgggttgg tgttgttgat gtggatcagc accttgcggc cgcgatacgg cgccagcgcc
    13621 cggatcatgc cgtcctcgcc ggactgcggc agatggccca tctcgcgcgc gaacttgtgc
    13681 acgatgccgt ggcggatcat ctcgtcctcg gtccagaagg tgccgtcgac cagcagcagg
    13741 tcggcctgtt cgaggtaggg caggaggtgg tcttcgattt cgccgaggcc gggcgcgtag
    13801 aaaagcctgc cgccgctgcg ggtgtcctcg atgaacacac cgacgttgtc accgggatgc
    13861 gggtcatggc ggtgcggcga gtacggcggc gccttgctcg ccagcgcgac ggtgcggatc
    13921 acgagccctg cggcaccggg aatcgtgaac gccgcgccat cgagcggcag ttcgtgcact
    13981 tcggtgccgc agtagtgttc gagcaccttc agcagcggca ggccggtgga gaggtcctgc
    14041 tgcgccggcg cgctcaggta caccggcagc cggcgcgtgt gttcacgcag catcagcagg
    14101 ccggtggcgt ggtcgatctg gctgtcgatc agcaggaccg cgcagatgcc ggtgtcacgg
    14161 atgccgcggt tcggctgcat cggcgcgaag gccgcgagct gggcgcggat gtcgggcgag
    14221 gcgttgaaca ggatccagtc cgtgccgtcg gcactgaccg cgatcgagga ctgcgtgcgt
    14281 gccgtcgccc gcagcgtgcc ggcgcggtag ccggcgcagt tcgcgcagtt gcagttccac
    14341 tgcgggaaac cgccgccggc gccggcgccg aggacgtgga tgtgcatgtc ggtgtcccgt
    14401 gggtggtcgg gcagggcggt gcggtgggcc gcactctcgg gagagggatg gccgggcggc
    14461 agcggcactg ccggtcctgg gccgaatgaa aacgccggcc cggaaggtgg cccgggccgg
    14521 cgtcgggcgg atcagctcag cgattggcga tgtacatcgt cacttcgaag ccgaaacgca
    14581 tctcggtgaa ctcgggcttg ctccactgca tggtgatctc ctcggtgatc ggggtcgtga
    14641 ttggggttct gggtccggcg actgccgccc ggcagtttta gcacgtcgca tgccagtcgg
    14701 tcagccgcgg aagcccgatc aaaaatgcat ggaaggtcaa aggtttattt cgaatcatgc
    14761 gacgtttgtc gttgtgcgct cgcagcatcc ggaccgtccg ggcggacaga agcgccggcc
    14821 ggcgggacag agtgtgtcag tcgaggcacg ccggcgccct gcgggcggtg ccgggaggcg
    14881 aacgtgggaa gacgcgcaaa aaaacgggcg accgtgtcgt tcggtcgccc caagagtccc
    14941 ggatcgcggg ggtcaagcga ggaggagtgc gagccccggc gctggaggtc ggccgacacc
    15001 ggaaaggggg ggacggtgcc ggcacggcgt cagaactcgc agtttgagta tcagcaatgt
    15061 gcatgccagc cagcggggag cggcccggaa tgcgggtttg cggctgtcgc cagcggcccg
    15121 ccgcggccgg gggcgggggt ggtgtcccgg gaacgatgcg acggtgtccc ggccggtgtt
    15181 cgaatccggg acgccggtac agcaacaggt ctgggcggga ctgctcagga cagcgtcggc
    15241 ggcgtgatgc cgtgcttctt gagcttgcga tagagcgtgt tgcgcgacag tccgagatcg
    15301 cgtgcggtgt tggtcatgtt ccagcggtgg cgttccagct cgcgcagcag cgcggccttc
    15361 tcggcactcg ccagcgaacc ttccggatcg gtcgcggcga tcgtctcgac cggcggcgac
    15421 acgaggcgcg gcgccgcctc ggcggcgccg gggccggcca tgcccggagc gcggccgagt
    15481 tcggcgggca ggtcctcggg gcggatctcg gcgtcctcgc acagcgcgac ggccgtgcgc
    15541 aggatgttgc gcagctggcg gatgttgccg ggccaagggt attcgagcag caggttgcgc
    15601 gcctcgaagg acagctcgac cggctggccc tcggcctcgg cggcgagcac gcgctcgatg
    15661 acgatggcga gatcggcgcg ctcgcgcagc gccggcagcg tcagcaccag accgttgagg
    15721 cggtagtaga ggtcctcgcg gaagctgccg ctggcaacga gatccggcag gttgcggtgc
    15781 gtggcgctga tcacgtgcag gtcgaccatg atcggtgcct cgccgccgag cggcacgacc
    15841 tcgcgctcct ccagcacgcg cagcagccgc gtctgcaggt gcgccggcat gtcgccgatc
    15901 tcgtcgagga acagcgtgcc gccgtgcgcc tgcacgatct tgccgcgcat gccttcgcgg
    15961 cgcgcgccgg tgaaggcgcc ggccttgtag ccgaacagtt cggattcgat caggttttcc
    16021 gggatcgacg cgcagttgat cgcgacgaag ggcttgtcgg cgcggtcgct ggcatcgtgc
    16081 acggccttgg caaaggcctc cttgccggtg ccggtctcgc cgttgagcag gatcgtgacg
    16141 ttgcgatcga cgaccttgcg cgcgcagcgc acgttgtagg ccatggccgg gtcctcgccg
    16201 gcgaggcctt cgaggtccat gcgcggcgcg atcaccgcgc tcatcgtgcg cccgggggcg
    16261 ggcgcggtct gcggggggat tgccgggcgt gccgccgcgg tgctgcggcg cgcggcgggc
    16321 atcgacatcg ctccgcgttc gagggcgctc tcggggccgc gcagcatcgc gtaatagccg
    16381 tgaccgcggc ggtcgagcag cggccagacg gtggcgtgca cgctggcgcg gcccatcagc
    16441 acgtcgaggc tcaggtcgaa gatgtcggcg acgcgcaggt gcatgagttc ttcgcgcgac
    16501 aggccgccga gctggttcag cgcgctctgg ttggcgccga gcacgcgacc gtcgccgtcg
    16561 aaggcgagca ggccctcggc gatgacgccg atgtactcgg gccgggcgtg gaagcgcagc
    16621 accgaggcct ggcggaagtg gcgctggaac gcgcagttct cgatggtctt ggccgacatc
    16681 gccgccagcg ccagcgcgtg gaactggctc tgcttggagt cacgcgaatt gaccgtggac
    16741 gcatcgagca cggcgagcag gttgccggcc ggatcgaaga tcgtggcggc cgagcagctc
    16801 aggccgatgt ggcgggcgag gaagtgctcg tcgcggtgca cggtcaccgg ctggcgctcc
    16861 atgacgcagg tgccgatgcc gttggtgccc tcgtggcgct cgctccagtc ggcgccgagc
    16921 cacaggccgg cgcgctggaa catgcgcgcc aggcccggcg aggtcacgca gttgaggatc
    16981 acgccgtcgg catcggtcag gatgatcgcg ctgccggagc cggcgatctg ctggtacagg
    17041 cggtccatct cgacgcgcgc cagctcgacg aagtcctgca gtttctcctg gcgctgcgca
    17101 agggccggcc ggtcgagcac cagcgtgccg cgcggcagga tcggttcgag gccatggttg
    17161 gcgatgcagc gctcccacga gcgcgagatc gccgcgtcgg gggccgagcg gccagcacgg
    17221 gcatcgccgt cccgggcaac cgacagcacg cgagcggcgt gctgcgacgc aaggcggtcc
    17281 aacatgctct cctcctccgc cgcgccgggg ggcacggcct tgtcgttgtg ggcgtatcgc
    17341 cgggcacggc attacgccac gcgcggtaaa acctgattct aaacccgttt tctgccgccc
    17401 tgcgaaatgc ttgccggtgt gcggaacagc cgtgaccagc cgtgacagtg cgtaccggaa
    17461 ctgtcacagc cggccatgtg ccatcgccgt ccggccggga tggccgggcc atttgcaggg
    17521 tctgaacgtg ttgtccgtgc gcaaaaatgc tctggatcaa tgatttggcc tttgcatttt
    17581 gctcatggca cgctcattgc tgaattgcgt ggcgttcgaa caccccctca tcccataaga
    17641 cccggaggag agcttggcga tgatgaagat cacgatgcgt gcaggtctgg tgctggcgac
    17701 cctgctgacg gcggccaccg cccacgccca cggcgacgtg accccgcagg cggtcgacac
    17761 caccgggctg gacccgctcg gcgacgagaa ctggcgcgag agcaatccgt accgcggcaa
    17821 cgaactggcg atcaagatcg gcaagtcggc gttcgcgcag aactgcgcgc gctgccatgg
    17881 catcgacgcg atctcgggcg gcatcgcgcc cgacctgcgc aagctggaaa agggcgcgct
    17941 cggcgacgaa tggttccagg agcgcgtgcg caacggcgcc gtgcgcaacg gcgccgtcta
    18001 catgccgaag ttcgcgaagc ccgaaggtgc gctgtcgcag gaagcgctgt gggcgatccg
    18061 ctcctggctc gacacggtcg cgatcgactg aaccacggct gaaagccttg ggggcgggcg
    18121 cggcggatgc ccgcgcgccc cgagccgcca gcgcccccgt ccggccgatc cgccgggcgg
    18181 gggcgctgtc tttagggctc cggcgtgcgg atttccgggc gcccggaggg cggtgttaga
    18241 ctcgcgcccc cgatttttcc ggccgtcccg gcaccgccgc cggcacggca caccgtatct
    18301 tctggagacg cagcgcatgg ccggccacag caaatgggcg aacatccagc accgcaaggg
    18361 cgcgcaggac gccaagcgcg gcaagctgtt cacccgcttc atccgcgaga tcaccatcgc
    18421 cgcccgcagc gggctgccgg acccggccgc caacccgcgg ctgcgcctgg ccgtcgacaa
    18481 ggcgctcggc gccaacatgt ccaaggacac catcgagcgc gcgatcaagc gcggtgccgg
    18541 tgccgatgac ggctcgacgc tggaggagat ccgctacgag ggctacggcc cgggcggcat
    18601 cgcggtgatg gtcgactgca tgaccgacaa ccgcaaccgc acggttgccg aagtgcgcca
    18661 cgccttctcg aagtgcggcg gcaacctcgg cacctcgggc tcggtggcct tccagttcac
    18721 cgagaccggc gtcatcagct acccggccgg caccgatcag gagaaggtca tggaggtcgc
    18781 gctcgaggcc ggtgccgatg acctcgtcga gaacgaggac ggctccttcg acgtgctgac
    18841 cgcgcccgat gccttcgcgg ccgtccggga agcgctcgac ggtgccggac tcgtggccga
    18901 gagcgccgag gtcacgatgc gcgccggcaa caccgtcgcg ctcggcctcg acgatgcgcg
    18961 cagcatgatc aagctgctcg acatgctcga agacctcgac gacacgcaga acgtctattc
    19021 gaacgccgac atccccgaag cggtgatggc gcagctctga agccggtttc cggcgccatg
    19081 cgcatcctcg gcgtcgatcc cggctcgcgg ctgaccggct acggcgtcat cgatgtccac
    19141 ggtccgcgcg ccgtctacgt cgacagcggc tgcatccgca cgccggccgg cgcgctcggc
    19201 gagcggctgt gtgcgatcca ccgcggactc gccgaaatcc tgcgcgtgca tgcgccggcg
    19261 gccgccgcca tcgagcaggt gttcctgcac cgcaacgccg tctcggccct gaagctcggt
    19321 catgcgcgcg gcgtggcgct gctcgcgctc gccgaggccg ggctcgcggt cgccgaatac
    19381 atgccctcgg tcgtcaagct cgccgtggtc ggcaccggcc gcgccgacaa ggaacaggtg
    19441 cagcacatgg tcaccgcact gctgaaactg ccgcgtgcgc cgcaggccga tgccgccgac
    19501 gcgctggccg tcgcgatctg tcacggcacg cagctgttcg cgcaggcgcg gctcgcgggc
    19561 gcggggcagg ggcgatgatc ggccggctgc gcggccggct gatcctcagg cagccgccgc
    19621 tgctgatgat cgaactcggc aacggcctcg cctacgagct cgaagcctcg ctgtcgacct
    19681 gctgcgcgct gcccgagacc ggcgccgagg tcgtgctgta cacgcatctg gccgtgcgcg
    19741 aggacgcaca ggtgctctac ggcttcggga cgctcgccga gcgcacgctg ttccgcaacc
    19801 tgatcagggt caccggcatc ggcgcgaagc tcgcgctggt gatcctctcc ggcatgagcg
    19861 tcgatgcctt cgcgcgctgc ctggaagccg gcgacgtggc gacgctgatc cgcctgcccg
    19921 gcatcggccg caagaccgcc gagcggctgg tggtggagat gcgcgaccgc atccagtcgc
    19981 tggaactcgg cgccgagttc gcgtcgcggc tgccggcgcg cgcgccggac gcgccgcccg
    20041 acccggtgca ggatgccgag gcggcgctgg tcgcgctcgg ctacaagccg gccgatgcga
    20101 cgcgcatggt ccgggccgtg gccgccgacg gcctgccgag cgaggagctg atccgccggg
    20161 cgctggcggc gacgctgtca cgcggctgag cgtttctggg cagtgctcag gccttgcgca
    20221 gcacgaacac gtagtggccc ccggatgatt cggtgaattc cagcagttcg tgaccgaagc
    20281 ggtcgatgaa cacgcggaaa tcgacgacga cgctgcggtc ggtcgcgagg atgcggatgc
    20341 gctcgccgga ctccatcttc gccatcgcct gcttggtctt cagcagcggc agcggacaga
    20401 gcagtccggt gagatcgagt acggtttcgg cggcggcagg cgtatccatg accgtgcatt
    20461 gtccgcacaa cggccggcgc gcgctaccgg tgacagcgaa gcccgtgcgc aggccggggg
    20521 agccctgccg ccaccggcgc cggcagcggg cggcctcgat gcgaatgacg gcggaagcag
    20581 ggcgggcggt gcgcgtggtc gtccgcaccg ccggcctgcg cgctgcagcg cctgctttcg
    20641 cgatgcgttt catccagccg tcatcgtttc cacgcggtca cgctgtgacg atggcttgcc
    20701 ggccggacgg atttcaggga ttcagcgtct tcgcgggcgc acgggccgtg tccgcgcctt
    20761 ccgggtcggt gacgcatggc agcagcggtg cgtggcccgg ctcttgctct tacgatggac
    20821 ggcgcgacgc aagtcgacgt tttgcgccat cttgatccgg acccgtgtcc gtccggctgc
    20881 cgagtgcccc gaatccgtca tgatcgaatc cgaccgcctg atctccgcgg ctgccagtgc
    20941 cgacgatgcg gcgatcgacc gcgccatccg cccgaagcgg ctcgccgact acgtcggcca
    21001 gcaggcgctg cgcgagcagc tcgaaatctt catccatgcc gcgcgccgcc ggcgcgaggc
    21061 gctcgatcac gtgctgctgt tcggcccgcc cggactcggc aagaccacgc tcgcgcacat
    21121 catcgccgcc gagatgggcg tgaacctgcg cagcacctcc ggaccggtgc tggagaagcc
    21181 cggtgacctc gcggcgctgc tgaccaacct cgaaccctac gatctgctgt tcgtcgacga
    21241 gatccaccgc ctgtcgccgg tggtcgagga agtcctgtat ccggcgatgg aggactacca
    21301 gatcgacatc ctgatcggcg agggcccggc cgcccgctcg atcaagctcg acctgccgcc
    21361 gttcacgctg gtcggcgcga ccacgcgcgc cggcctgctg accagcccgc tgcgtgaccg
    21421 cttcgggatc gtgcaccggc tggagttcta cacgaccgcg gaactcacgc agatcgtcgg
    21481 ccgctcggcc ggcatcctcg gcgtgccgac cgatgacgac ggcgccggcg agatcggccg
    21541 gcgctcgcgc ggcacgccgc gcatcgccaa ccggctgctg cgccgcgtgc gcgactacgc
    21601 cgaggtcagg gccggcggcc acatcacgca ggacatcgcc cgcgcggcgc tggcgatgct
    21661 gaaggtcgac gaacagggct tcgacgacat ggaccgccgg ctgctcggcc tgctgctcga
    21721 caagttcggc ggcggcccgg tcgggctcga cacgctggcc gccgcgctcg gcgaggagcg
    21781 cggcacgatc gaggacgtgc tcgaaccctt cctgatccag cagggcttcc tgatgcgcac
    21841 gccgcgcggg cgcaccgcga ccgcgcacgc ctaccggcac ttcggcctgc tgcccccggg
    21901 cggcgccgac ctgttcgacg caccggtctg agcacgcgca tggccgtgac ccgacatggc
    21961 ggccggcgct ggccgggatc cctgcgcagc ctgatcatcg ccggcttcct gctggcgctg
    22021 acgccgccgc tcgccgcgct cggctgggcg gcgtacacgc tcaattccct ggtcgccgag
    22081 gtcgatgccg ggctggaaac ggcagtcgcc gcagcccgct acggccgcgg tctggtcgcc
    22141 gatgtcaccg agctgcagcg ctcggcacgg cactggaacg tgctgcagtc cgacacgctg
    22201 ctcgacacct accgcgagcg gcgcaagcgc gtcgacgaga cgctcggcca gctgcgggcg
    22261 ctcgcgctcg accccgaacg cgtgcagcgc ctcgaacgtc tggcggcggc gctgccggtc
    22321 ggtgacggtc cgccggtcgg cgatgacgac gtgctgaccg cgcagctcgc gccgctggtc
    22381 gcgcaggcgg tcgacgtgct ggagctctcc gacgacgcgg tcgagggcga gagcgcgcgg
    22441 ctgcgcgagg tcacggccga ggccaaccgg cgcttcgcgt cgctggccgc gctcgggctg
    22501 atcgccggca gcggctgtgc gctgctcgcc ggcggcctga tcgcacggcc gacgcgccag
    22561 ctcgcgcagg cgatccggcg cctcggcgac gaggatttca cgacgccggt cagcctcgcc
    22621 ggaccgcgcg acatccagct gatcggcgcg cggctcgact ggctgcgcag ccgcctcggc
    22681 gagatcgacg cgcagaagca gcgcttcctg cgccatgtct cgcacgagct gaagacgccg
    22741 ctgaccgcgc tgcgcgaggg ttcggagctg ctgcgcgacg gtacgctcgg gccgctggcc
    22801 gaggaccagc gcgaggtcgc cggcatcctc gtcgacaacg cccggcggct gtcgacgctg
    22861 atcgaggacc tgctgaactt caaccgcctg ctggcgcgca cgcagcagct cgaagcgggc
    22921 acggtggcac tcgccgcgct ggtcgaggaa gtcgccggcg cgcagcgtct ggccgcgcag
    22981 gcgcgctgcg tcacgctcga gctcgacgtg ccggaggggc tcatcgtgca ggccgatcac
    23041 gacaagctgc gcacgatcct cgacaacctg ctctccaacg ccctgaagtt cgcccggcgc
    23101 gacggccgca tcgcactgcg cgccggccgc tatcccggcg gcgtcgccgt cgaggtcgag
    23161 gacgacggtc ccggcatccc ggccgaggag cgcgagcggg tgttcgagct gttcttccag
    23221 ggcagccacc agccggatac cccggtgcgt ggctccggtc tcggcctcgc cctggcccgt
    23281 gaattcgccc gcctgcatgg gggcgacctg atcgtcgccg atggccgcgg cagtggtgcc
    23341 tgcctgtgcc tgacgctgcc ggcgactgca ccaccgtagc gcgcaaaaaa atccggcggg
    23401 caccgggaca ggactcttga accccggggg atcgcctaca agtcgcgcca cgccaaggct
    23461 ttttttcttc caccacccgc tcggtgaccg caacatgcgc cgccactctc cgcccctcct
    23521 gctctgtctg ctggccgtcc tcggcaccgg ctgcgacacc ctcaacggca tgaccgacaa
    23581 gccgccctcg ggcgacgagg cgatcctgcg caagcaactg gtcgatgccc gcagcgtcgc
    23641 cgaccgcgcc cgcgccagcg cccgcaaggc cgaggcgcgc gcgaccgatc tcggcaagga
    23701 caaggccgat ctcgaaacca ccgtcgcgga actgcgcgcg cagaacgagc gactgactca
    23761 ggagaatctc gatctgaagc gccgtctgca ggcgctcggc cggatcgaat ccacgctttc
    23821 cgaccagggc aaatgacgtg accgacaaac gctgcgacat cctcgcggtc gacgatgatc
    23881 ccgatctgct gcgtctgctg gtcctgcgcc tgaaggccgc cggctacgtc gtcgaaaccg
    23941 ccaccaacgg tcaggaagcc ctgacccagc tcacgctgtg cgcgccgcgc ctgctgatca
    24001 ccgacctgcg catgccgggc atggacggcc tggcgctgtt cgacgccgtg cgcagccagc
    24061 gtccggggct gccggtgatc atgctgactg cgcacggcac gattcccgat gccatccagg
    24121 ccacgcgcga cggcgtgttc ggcttcctgc cgaagccctt cgagccggcc gaactgctcg
    24181 ccgaggtgca gcgcgcgctc gcgaccacgg cgccggcgcg cggcaacccc gccggctggc
    24241 gcgcggcgat cgtcacgcgc agcccgcggc tcgaatccgt gctcgacgag gcgcagct
//
LOCUS       DAOQIE010000015        23509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_955_length_23509_cov_7.434340, whole genome shotgun sequence.
ACCESSION   DAOQIE010000015 DAOQIE010000000
VERSION     DAOQIE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 23509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23509
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_955_length_23509_cov_7.434340"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..2842)
                     /locus_tag="PK144_01925"
     CDS             complement(35..2842)
                     /locus_tag="PK144_01925"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNF64512.1"
                     /translation="MDKTYEPHAIEQCRYETWESQGRFAPQGSGAPYCIMLPPPNVTG
                     TLHMGHAFQHTIMDALIRYHRMNGDRTLWQPGTDHAGIATQMVVERQLNAEGKSRHDL
                     GREAFLARVWEWKAYSGGTIGRQMRRLGTSPDWSRERFTMDEGLSAAVQEVFVKLYED
                     GLIYRGQRLVNWDPVLHTAVSDLEVVSEEESGSMWLIRYPLTDGEDALVVATTRPETL
                     LGDTAVAVNPQDERYRHLIGRSVRLPLCDRLIPVVGDDYCDPAFGTGVVKITPAHDFN
                     DYAVGQRHGLTLINVFTPDAKLNDNAPEAYRGLDRFEARKRIVADLEALGLLVEVKPH
                     RLMVPRGDRTGAVIEPFLTDQWYVDLTRREQADGRPGGWTAIVEPALAAVADGRIRFV
                     PENWSRTYNEWLNNIQDWCISRQLWWGHRIPAWYDETGHIYVGRSEAEVRTRHGLTPE
                     HALRQDEDVLDTWFSSALWPFSTLGWPQQTEALKTFYPTSVLVTGFDIIFFWVARMVM
                     AGMYFMEGQVPFREVYITGLVRDGEGQKMSKSKGNVLDPIDIIDGISLDELVAKRTAG
                     LMQPQMAAKIEKRTRKEFPEGIHAHGTDALRFTFAALATNGRDINFDMGRVEGYRNFC
                     NKLWNAARYVLMNTEGQDCGLNGKCGYSLADRWIQSRLQATIREVRDGIDNYRFDMAA
                     KAIYEFIWNEYCDWYLELAKPVLTGDGQDIGSHEIMKRGTRRTLVGVLESVLRLLHPL
                     MPYITEEIWQRIAPLAGTHSETLSLASFPAVDDRLTDVGAEAEIAWVQTFLVGVRRIR
                     AELNLAPGRSLQVLARGGNALDHERLDRNMLFLKTLGRLDSITTVIEAPESATTLVGS
                     MEILIPLAGLIDKDAELTRLAREIDKINKDIERIEAKLANPAFADKAPAEIVGREREK
                     LELQRSALNKLMETQSRMASL"
     gene            complement(2959..4353)
                     /locus_tag="PK144_01930"
     CDS             complement(2959..4353)
                     /locus_tag="PK144_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF64513.1"
                     /translation="MSESAPDYRDIIRQVAEHAGHLAIAITDITGHIGEVSERAMREA
                     EAFGTLQGLATHMASQNTAVAEAADSTQRVVGTARSEVDRTRQTIESSLADIRALTES
                     VATIEVQLAELGGAMERVSAVAKGIDAIAKQTNLLALNATIEAVRAGEAGKGFAVVAS
                     EVKELAKQTRSATAEIDVTLRELGERTRGLLAQGAQSKTRSEEVRRGTQAIQQVMDTM
                     GAAMQDVDDQTRRIGDAVHEIAGSCQQTVTALNQVAESVHSSSANLRQARDQLTSVQA
                     VSEALVGTTAVDGIETVDAKFIAMAKDTAKRIGELFEQALTRGDLNESDLFDRQYRPI
                     PGTDPQQHLTRFTDFTDRVLPSLQEALLDRDRRIAFAAAVDDNGYLPTHNRKYSHPQG
                     RDPIWNAANCRNRRIFNDRTGLASGRNQKPVLLQTYRRDLGGGKFVLMKEVAAPIRVR
                     GRHWGGFRLGYIPD"
     gene            complement(4490..4915)
                     /locus_tag="PK144_01935"
     CDS             complement(4490..4915)
                     /locus_tag="PK144_01935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNF64514.1"
                     /translation="MATRIEFYVLATAGEAACLATACRLVDKARSQGLSVWIRTASAA
                     QSATIDRELWTWRRGSFIAHRIHAPGRRPWPVLIGTGAPPESHRALLLNLADDVPDGF
                     EACSRLIEIVDAREDILAASRARYKVYRDRGFAPSTVRL"
     gene            complement(4929..6437)
                     /locus_tag="PK144_01940"
     CDS             complement(4929..6437)
                     /locus_tag="PK144_01940"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNF64515.1"
                     /translation="MEFSVKSGNPEKQRTACLVLGVYESRRLTPPAEQFDTAAEGFLG
                     NVLRRGDLEGKPGQTLLLVNTPATLCDRVLLVGCGRERDLDDRRYKQIIAAAVHTLND
                     TGATEAVSYLTDLNVRGRDVAWKVRLAVEVSEDVLYRYDETRSRKDAPRRPLKRIVLA
                     VPSRRELPLGEQAVREGAAVAEGMKLARDLGNLPPNICTPEYLAQRAALLAGSSPKFA
                     LNVLERADMETLGMGCLVAVARGSRRPPKLIALEWHGAADARQKPVVLVGKGITFDTG
                     GISLKPGPDMDLMKFDMCGAAGVLGTMSAIAALDLPLNVVAVVPAVENMPGGEASRPG
                     DIVTSLSGQTVEILNTDAEGRLVLCDAITWAEQRYEPVTLVDVATLTGACVIALGAHP
                     HGLFSNHGPLANELLDAGRETHDRAWELPLWEDYQDGLDSNFADMANVGPREGGAITA
                     ACFLARYTKKLHWAHLDIAGTAWKTGKQKGATGRPVPLLTRFLINRAAAARD"
     gene            6595..7689
                     /gene="lptF"
                     /locus_tag="PK144_01945"
     CDS             6595..7689
                     /gene="lptF"
                     /locus_tag="PK144_01945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNF64516.1"
                     /translation="MLRTFDRFLLRETAAVTAATLLVLLAIVLSSRLATYLTQVASGL
                     LIKEVILQMLVLQAIRFLPVLVPIALMIGVLLVLGRLHQESEMAALTGCGYGPAELYR
                     PLLALALPLAALVALVVLGVAPWAASLQFELQARARQQAQMSLFVPGTFREAAGGQHV
                     VYVESLDPIDGELVKVFIHSTEPDSGRIAVTTGNRGHQETGADGVRYMVLERGFRYEG
                     EPGLTDYRQVHYEHLRVRIDEPASGDVSRKRESVPTAQLLRGDRRDKAELHARIGAIV
                     SIPLIVFVAPLLARAQPRESRYARILGGVLVYAIYMNLLGIGKAWLEKGSIGPVAGLW
                     WAHLLFLALGLALWFHHYGRHGLTVSWRRA"
     gene            7686..>8597
                     /gene="lptG"
                     /locus_tag="PK144_01950"
     CDS             7686..>8597
                     /gene="lptG"
                     /locus_tag="PK144_01950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNF64517.1"
                     /translation="MKRIDAYILRTVIGASLIALLVLVALETFFTLLVELEDLGTGQY
                     GLAEILRYLALTLPRRTYESFPMALLLGGLMGMGALAAGSELVVMRGAGVSILRLVGS
                     AMRAGLLLSLFALALGEFVAPRTERLAETGRSTAKDASVTIRDGRGFWARDGALFVNV
                     RAVKPHKRLADITLFEMAPDSSMKAVIRAPSADYDPASRRWVLRDARRSTLGEERVTA
                     DAQVSFAWESVLDPGKLDVLAAKPETLAMRQLQTFAGYLRDNGLSADRYELAFWSKAL
                     APLANLTMLLIAMPFVFAHQRNAAAGQR"
     assembly_gap    8598..8697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8975..10471
                     /locus_tag="PK144_01955"
     CDS             8975..10471
                     /locus_tag="PK144_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF64518.1"
                     /translation="MTKNPPETSRRNAFLHAHPWFVPQASAAELGSGQPVLLLDDTSG
                     LLRSVSAMLNAAGVPAVIGETDPQRLPARLSEQGCGVLLVDASLPELEPEVLLARLSA
                     EQPDLPVIVLTEAHDPEAAMRCLQAGAFDFLVKPVDRTRLLASVGRALEIRGLKHELA
                     SLTQTMLDDRLDHPEAFARLIAQDRSMRAACRYIEAIAPLSQPVLITGETGTGRKLLA
                     QALHAVSGRPGACVMVEVGGMDERRFAETLFGHRPGLHAEAGQPQEGRIGAAADGTLV
                     LLEVGELAPAAQQLLLNLLQERSFSPVGSGIVQECRARIVAISRHDLAHQADSGRLRK
                     DLYYRLRAHHVHVPPLRSRRGDVPLLVEHFVARAVEALGKGDVHIPAELYSLLRNHDF
                     PGNVAELEAMAYDAVASHRSGGTLGLASFRHCVSSTPDAPPPPPPVQSLSQLFPDRLP
                     TLKEAEAFLVAEALRRADNNQGIAAGLLGLTRQALNKRLVRGRQSSSH"
     gene            10582..11679
                     /locus_tag="PK144_01960"
     CDS             10582..11679
                     /locus_tag="PK144_01960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNF64519.1"
                     /translation="MLSAPLPPDEAERLYALRSLDLLDTPREERFERITRLAVRLFGL
                     PIATVSLIDGDREWLKSVQGLALREIAREHSLAAHAILDNEPMIVSDAQADPRFGDHP
                     LVLGEPRFRFYAACPLHAGRRRHRVGTLSVAGRIAHRLAEADAQALRDLAAIAEAEIG
                     RSLASEVQSQLLRERDTARRQALLDALTGLWNRKAILDLLARELVRCEREGIGVSVVL
                     VDLDHFKAINDNHGHLTGDEALRAASAAMLQALRPYDALGRYGGEEFLIVTTDPEGGP
                     VAVAERIRARLAEVRVATGSGGVVALSGSVGVARCPAGERRELEDLLRAADRALYAAK
                     HAGRDRVVTEDAANSDEAPVQPDGSSTDNAA"
     gene            complement(11643..12062)
                     /locus_tag="PK144_01965"
     CDS             complement(11643..12062)
                     /locus_tag="PK144_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNF64520.1"
                     /translation="MSSGLWSLFAGSFLAATLLPGGSELLYAALLLRGEDSPLALLAV
                     ATLGNTLGGLSSFAIGRWLRQRRPAGPFAARHEQALTRLRRFGAPALLASWVPVIGDP
                     LCVAAGWLGIGWTAATLWIALGKAARYAALSVLLPSG"
     gene            complement(12102..12791)
                     /locus_tag="PK144_01970"
     CDS             complement(12102..12791)
                     /locus_tag="PK144_01970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNF64521.1"
                     /translation="MKLPPLPPYRVREHARARRVRLRVSPAHGIEVVVPSGFPARELP
                     RLLEPHRHWMEMQLARCRAGLELPAVMLLPALGETWSLRREALPEGRRARLLVEAGAL
                     HLQAADAAAARALLQRFVQRRAAEVLPPWLARVAEETGLRFAGVRIRTQRARWGSCSA
                     SGRISLNCKLLFLEPALVRHVLVHELCHTVHLNHSPAFWKVVADHDPDWRRLRAELQA
                     RPAMAPAWIEA"
     gene            complement(12788..13276)
                     /locus_tag="PK144_01975"
     CDS             complement(12788..13276)
                     /locus_tag="PK144_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF64522.1"
                     /translation="MPAPLPPYHCTVEADWTDYNGHLNVAFYVLVFDRATDAALACCG
                     LDGALRESAGSTVFAAECHVSYQRELPGGAPLAVDTQILGLDDKRVHLFHTLGHAEAG
                     WPAASAEFLLLHVDLGSRRVAPWPPAVHARLAAVRSAHERLPVPAQAGRSVQLRRREP
                     QP"
     gene            complement(13284..14165)
                     /locus_tag="PK144_01980"
     CDS             complement(13284..14165)
                     /locus_tag="PK144_01980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNF64523.1"
                     /translation="MSTPQPGILAPIPAASRHLFFHVNACHDAPRALQALRGLVDGFA
                     VVVGIGAELAAGLDAPIPGLRPFPSLPGARVPIPYVPYALWCWLRGSDRGELFHTGRE
                     IENALAGVFTLELAIDCFCHRDSRDLTGYEDGTENPVDEAALAAAIVSHEGDGLDGGS
                     FASVQQWLHDIERFQRLSPGEQDDTVGRSRIDNEELEDAPDSAHVKRTAQEDFDPPAF
                     MVRRSMPWTAERRGGLVFLAFGHSPDAFEAVLRRMSGAEDGIHDALFNFTRPLNGAHY
                     WCPPLLDGRPDWRALGI"
     gene            complement(14230..14694)
                     /locus_tag="PK144_01985"
     CDS             complement(14230..14694)
                     /locus_tag="PK144_01985"
                     /inference="COORDINATES: protein motif:HMM:NF040030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HNF64524.1"
                     /translation="MPISPIDLPAGACGPFANECSDWLRACAPGRLLLLGDAGDLTLP
                     PGTEVDARADASALAADARYDAVLIRGLPELLDRRAGAALLARARDRLAPECLAVLPR
                     PCWDEGAVLGLGYTLRARAPAALLGHFAIASYKSVPDWFNSKFWAHPERWQP"
     gene            14809..15513
                     /gene="mobB"
                     /locus_tag="PK144_01990"
     CDS             14809..15513
                     /gene="mobB"
                     /locus_tag="PK144_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNF64525.1"
                     /translation="MMQGRGVIATGNGVPPVDAGLAAPDAAVAACPLPVLGIVGPSGA
                     GKTALLCRLLPELAAAGLRVGVIKQASARFDLDEPGRDSDRLRRAGLARTLVAAVGQT
                     ALIEERDPACQPGDCELADALGWLDVGALDLVLVEGFARAALPKLEVLRADGPKRHQY
                     RHDAWVRALLADHEPEPEPESEPAAAAAAASRLPRLDLADATAIAGWLCAYREEFLAR
                     RAGRTGGAARSGELAR"
     gene            15510..16820
                     /locus_tag="PK144_01995"
     CDS             15510..16820
                     /locus_tag="PK144_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNF64526.1"
                     /translation="MSLSCSPGSDAIRAQASCCDDHDPHSLPVPVALARIQTAVQPVT
                     ASEQVALRAALGRVLAADVVSPIDVPAHVNSAMDGYAVRAADLPDEGTVTLAVVGTAF
                     AGAAWDGRLASGQAVRIMTGAVLPEGADCVVMQEQTEREQGGDQEGGQDRVRIGRGHR
                     AGQNIRLAGEDLKTGETVLAAGRRVQPADLGVLASLGVAEVRVRRRLRVAFFSTGDEL
                     RSIGEPLAAGCVYDSNRYSLYGMLSRLGVELIDMGVVRDDREALQAAFREAAACADAV
                     ITSGGVSVGEADFVKDTLLSLGRIDFWKLAMKPGRPLAFGRLGEAVFFGLPGNPVSVV
                     VTFYQFVQPALRRMMGMNAVQPVTLQVPTLARLKKRPGRTEFQRGVLEQRDDGSMAVR
                     PTGDQGSGILSSVSRADCFIVLAADSSGVEAGDTVTVQPFEGLF"
     gene            16861..17250
                     /gene="crcB"
                     /locus_tag="PK144_02000"
     CDS             16861..17250
                     /gene="crcB"
                     /locus_tag="PK144_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNF64527.1"
                     /translation="MSGLGLLAVGLGAAVGAWLRWLLGLWLNPLFPPVPLGTLAANLI
                     GGLLMGIALGVFQALPALAPEWRLALATGFLGGLTTFSTFSAEIVTLLARGELVWGGA
                     AILMHVGGSLAATAVGLALVGWLRTLG"
     gene            17284..17622
                     /locus_tag="PK144_02005"
     CDS             17284..17622
                     /locus_tag="PK144_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNF64528.1"
                     /translation="MNPSESGIALRFHLSEGRRHHGRLLWEWLLELARELDLPGGTAY
                     RALAGYGRHRRLHEAHFFELAGDVPIDVIFILRPADEARLLERIDAEGLALFYARMPI
                     EFGLTGPAGG"
     gene            17731..19638
                     /locus_tag="PK144_02010"
     CDS             17731..19638
                     /locus_tag="PK144_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNF64529.1"
                     /translation="MPNDAALAVADAPHTPKKNLTTLTLAALGVVYGDIGTSPLYALR
                     ECFSGPHGIIATEANVLGVISLIIWSLLLLVSVKYVAFVLRADFRGEGGILALMALAC
                     SRRFATRRRHWSVLILGVFGATLFYGDAMITPAISVLSAIEGLNVATTVFEPWVVPLT
                     LLVLIGLFLVQSHGTDRVGKFFGPVMLVWFGALAVFGVAEMLKRPDILAAVHPRHAYF
                     FFRDNQWHAFVTLGAVFLVMTGGEALYADMGHFGRRPIRRAWFALVLPAILLNYFGQG
                     ALLLANPAAAENPFYLLVPAPLLYPMVGLATAATVIASQAVISGVFSLTRQAMQLGYA
                     PRMSIEHTSAAEIGQIYIPAVNWTLLVAVLLLVLGFESSSRLAAAYGIAVTLTMVITT
                     LLLAVVTRRVWGWHPALVAVCILPFLAADLAFLGANALKIPQGGWFPLVVSALLFTFM
                     TTWKRGRKLLARRLREEAIPIKLFVDDAADGMIQRVSGTAIFLTSNPDGVPHTLLHNL
                     KHNKVMHERVVLLTVRTEEVPFVREDKRVEVETIGSGFYRVHMRFGFTEDPDVQAALA
                     LCGPHGLEFELMETTFFLGRETLISTSRPGMARWRERLFIAMSRSSARAMDFFNIPIN
                     RVVELGTQIEL"
     gene            complement(19868..22381)
                     /locus_tag="PK144_02015"
     CDS             complement(19868..22381)
                     /locus_tag="PK144_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64530.1"
                     /translation="MSLSPGIDLNGVCDCTVLPDGALRLAAAPPVVIDASPLGVLTGR
                     MALDAPAGRSGWPAEDGIRLPVLSLLRALGHDDDAPLDAHEVDAPEAARLLGRHLAGL
                     LPARVRTAVAAVPDTDGFDERTRARLLDGAARAGIDLSLLWRPVAALLGWGEALDARA
                     LQALHGRSACVMQLLADGIALGSYGLEAVEQGGRLTLVPVRRRDGLRSWQPWPHGNLS
                     RLLARELDCAEPALCSGPWAWNVLLGRAGQRELQPDAQAPGGWRLLQGPAHLGGALAD
                     ALRRGLDAVLASTPAALQDAALILIEGPLNDAALATDGAAPLAFGHYVQKRLTSALGS
                     STSRRIVTAGLRDALVARGCSVSATRLGAREIAYYDFLPMLEINVLRAGEHAFAELIE
                     PAERVAGGSSYTHTLADRFIVAKDTRSLVFYLLKEDEAGARRSETLLPVPPSTTVEIS
                     LQVTQTPAQGYARVEIRPTKSGILGQSAILLDWTTMTAITGSREDVLLELELQGLGHP
                     DIAPQRTHRLLWKYKRGDGLTILKALTEFTASPLKTTAHSQYKKLLVQTRSLAGLRTS
                     PYLLTQGANPDRTACTAFDSDGNIPEEFALQMQPCIEQFCNCLNSDFSAVKHARTRQD
                     IQTRRNLSRLGATLYAACPDEIVHYYRLIARGTVSDPTLVLHAGKVLCTEADLDSLFR
                     YCADCSISAVKDGKKLSNFVLRAAGDALAYRETAGVVLNAISADRLADAALMVLEEEI
                     NAINYKVRFRAAARLALFILRHRMRRRDFLHPSSRDPANRRRAQRLEDLITRSLASRH
                     AGDDLTVALQELQAQIRYRGTNAIIDIDADAGDETEDED"
     gene            complement(22378..>23509)
                     /locus_tag="PK144_02020"
     CDS             complement(22378..>23509)
                     /locus_tag="PK144_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HNF64531.1"
                     /translation="EHAGRLPALVEATAGHPRRVLKRTRQQMPIDRIQELDTACLEWM
                     IRRPGLSVAEKAGPRQRLLGVAREENLDTLENRVLKDFLRLSIEEAGLWQHANQRFGL
                     SERARRVGRYQALCRNCHRELRAAGISDPVAPVLPNFVLQHDARYRVIWRAYRELLSA
                     EQREDDLWRWQGRLWADFVRLAVVIGLGRIADPQVRLQAPLRLRREQARGRWSDTLAR
                     LAVFLLTLRGRPLVAEVLDPGLPQPAAQPAGLAAVAPWQHALGCTAVIVLRDGRDGRQ
                     RSLCVWAAHGVTDVPLSLETLAASADEALDQAMRREALHGDDRIRARGLVIRSKHDAA
                     PEFATQAGRVYGLTLSADPARIREAFAEFALTLQDSLERLFA"
BASE COUNT         3381 a         7989 c         8262 g         3777 t
ORIGIN      
        1 taaaaagaat gccatcagca cggcagattc aaaattacag gcttgccatg cgcgactggg
       61 tttccatcaa cttgttcaac gccgatcgct gcaactccag tttctctcgt tcgcgaccga
      121 cgatttcagc cggcgccttg tccgcaaagg cagggttagc cagtttggct tcgatgcgct
      181 caatatcctt attgatcttg tcaatttcgc gggcgagacg ggtcagttca gcatccttgt
      241 caatgagacc cgccagggga atcaggatct ccatgctgcc gaccagcgtg gttgccgatt
      301 cgggagcttc gatcaccgtc gtgatgctgt cgagacggcc gagggtcttg aggaacagca
      361 tgttgcggtc gaggcgttca tggtccagtg cattcccgcc gcgggcgagt acctgcaagg
      421 atctgccggg cgccagattg agttcggccc gaatgcggcg cacgccgacc agaaaagtct
      481 gcacccaggc aatttcggct tcggcaccga catcggtgag tcggtcatcg accgccggaa
      541 atgatgccag cgacagcgtt tccgaatgtg tgccggcaag cggcgcgatg cgttgccaga
      601 tttcttccgt gatgtacggc atcagcggat gcagcaggcg cagtacgctt tccagcacgc
      661 cgaccagcgt gcgccgggta ccgcgtttca tgatttcgtg cgagccgatg tcctgcccgt
      721 caccggtcag caccggtttg gccagttcga gataccagtc gcagtattcg ttccagatga
      781 attcgtagat ggccttggcg gccatgtcga aacggtagtt gtcgataccg tcgcgcactt
      841 cacggatggt ggcctgcagt cgcgactgga tccagcggtc ggcaagcgaa tatccgcatt
      901 tgccgttgag cccgcaatcc tggccttcgg tgttcatcaa cacgtagcgg gcggcattcc
      961 agagcttgtt gcagaaattg cggtagccct cgacgcggcc catgtcgaag ttgatgtcgc
     1021 ggccattggt ggcgagtgct gcgaaggtga agcgcagggc gtccgtgccg tgggcatgga
     1081 tgccttcggg aaattccttg cgcgtgcgtt tttcgatctt cgccgccatc tgtggctgca
     1141 tcaggccggc ggtgcgcttg gcaaccagtt catcgagact gatgccgtcg atgatgtcga
     1201 tcgggtcgag cacgttgccc ttcgatttcg acatcttctg gccttcaccg tcgcgcacca
     1261 gaccggtgat gtagacctca cggaatggca cctggccttc catgaagtac atgccggcca
     1321 tgaccatgcg ggcaacccag aagaaaatga tgtcgaagcc ggtcaccagc acgctggtcg
     1381 gatagaaggt tttcagcgcc tcggtctgct gcggccagcc gagcgtgctg aacggccaca
     1441 gcgccgaact gaaccaggta tcgagcacgt cctcgtcctg acgcagcgca tgttccggcg
     1501 tcaatccgtg cctggtacgc acctcggctt cgctgcgacc aacgtagata tggccggtct
     1561 cgtcatacca ggccggaatg cggtgacccc accagagctg gcggctgatg caccagtcct
     1621 gaatgttgtt cagccactcg ttgtaggttc tggaccagtt ttcgggcacg aagcggatgc
     1681 ggccatcggc aactgccgcc agtgccggtt caacgatcgc tgtccagcca cccggacggc
     1741 catccgcctg ttcacgccgg gtcagatcga cgtaccactg atcggtcagg aacggttcga
     1801 tgacggcacc ggtgcgatcg ccgcgcggca ccatcagccg gtgcggcttg acctcgacca
     1861 gcaggccgag ggcttcgaga tcggcgacga tgcgcttgcg cgcctcgaag cggtcgagac
     1921 cgcggtaggc ttccggtgcg ttgtcgttga gcttcgcgtc cggcgtgaag acgttgatca
     1981 gcgtcagccc gtgacgctgg ccgacggcgt agtcgttgaa gtcgtgggca ggggtgatct
     2041 tgacgacgcc ggtgccgaag gccggatcgc agtagtcgtc gccgaccacc ggaatgaggc
     2101 ggtcacaaag cggtaaccgc accgagcggc cgatcagatg gcggtaacgc tcgtcctgcg
     2161 ggttgaccgc aactgccgta tcgccgagca gagtctcggg gcgggtggtg gcgaccacca
     2221 gcgcatcttc gccatcggtg agcggatagc ggatcagcca catcgaaccg ctttcttcct
     2281 cggaaacgac ttcgaggtcg gagacggcgg tgtgcagcac cggatcccag ttcacgagcc
     2341 gctggccgcg gtagatcagg ccgtcctcgt acagcttgac gaagacttcc tgcacggcgg
     2401 ccgacaggcc ttcatccatc gtgaagcgtt cacggctcca gtccggcgag gtgccgagcc
     2461 ggcgcatctg ccggccgatc gtaccgcccg agtaggcctt ccattcccag acgcgggcca
     2521 ggaaggcctc gcggccgaga tcgtggcggc ttttgccttc ggcattgagt tggcgttcga
     2581 cgaccatctg cgtggcgatg ccggcatgat cggtgcccgg ctgccacagc gtgcggtcac
     2641 cgttcatgcg gtgatagcgg atcagcgcgt ccatgatggt gtgctggaac gcgtggccca
     2701 tgtgcagcgt gccggtgacg ttcggcggcg gcagcatgat gcagtacggc gcgccgctgc
     2761 cctgcggtgc gaaccggccc tgggattccc aggtctcgta ccggcattgt tcgatcgcgt
     2821 ggggttcgta ggtcttgtcc atggcggggt cggttctggc ggcgcggcgt cgggaaagcg
     2881 gcgcagtata gcgaaggggc agggacgacg aagccggcgc agggccggct tcgtcggagc
     2941 ttcgaaggca ggacaggttc agtccgggat gtaaccgagc cggaatccgc cccagtgccg
     3001 gccgcgcacg cggatcggtg cggcgacttc cttcatcagc acgaacttgc cgccgccgag
     3061 atcacgccgg taggtctgca gcaggaccgg cttctggttt cgacctgaag cgagaccggt
     3121 acggtcgttg aagatgcgac ggttgcggca gttggcggcg ttccagatcg gatcgcgccc
     3181 ctgcgggtgc gaatacttgc ggttgtgggt cggcagatag ccgttgtcgt cgacggcggc
     3241 ggcgaaggcg atgcggcggt cgcgatcgag cagcgcttcc tgcaggctcg gcagcacccg
     3301 gtcggtgaag tccgtgaagc gggtcaggtg ctgctgcgga tcagtgccgg gaatcggccg
     3361 gtactggcgg tcgaacaggt cgctttcgtt caggtccccg cgggtcagcg cctgctcgaa
     3421 cagttcgccg atgcgtttcg cggtgtcctt ggccattgcg atgaatttcg catcgacggt
     3481 ctcgatgccg tcgacggccg tcgtgccgac cagcgcctcg ctgaccgcct gcacgctggt
     3541 cagctgatcg cgcgcctggc gcaggttcgc gctcgacgaa tgcacggatt cggcgacctg
     3601 gttcagcgcc gtgacggtct gctggcagct gccggcaatc tcgtggacgg catcgccgat
     3661 gcggcgggtc tggtcgtcga cgtcctgcat cgccgcgccc atggtgtcca tgacctgctg
     3721 gatcgcctgg gtgccgcggc ggacttcctc ggagcgcgtc ttgctctgcg cgccctgggc
     3781 gagcaggccg cgcgtgcgtt cgccgagctc gcgcagcgtc acgtcgatct cggcggtggc
     3841 cgagcgggtc tgcttggcga gttccttgac ctcgcttgcg accaccgcga aacccttgcc
     3901 ggcctcgccg gcgcgcacgg cttcgatcgt ggcgttcagc gccagcaggt tggtctgctt
     3961 ggcgatcgca tcgatgccct tggcaacggc gctgacgcgc tccatcgcgc cgccgagttc
     4021 ggcgagctgg acctcgatgg tcgcgaccga ttcggtcagc gcccggatgt cggcgagcga
     4081 ggattcgatg gtctggcggg tgcggtcgac ctcggagcgc gccgtgccga ccacgcgctg
     4141 cgtgctgtcg gccgcttcgg cgacggcggt gttctggctg gccatgtgcg tcgccagccc
     4201 ctgcagcgtg ccgaaggctt cggcctcgcg catcgcgcgt tcgctgacct caccgatgtg
     4261 gcccgtgatg tcagtgatcg cgatcgcgag gtggccggca tgttcggcga cctggcggat
     4321 gatgtcgcga tagtccggcg cgctctcgct catgaatgaa gctcctcggt ggatgcctgc
     4381 ggccactgcc gggggggcgg cgtcgccgtc tggttatgtc ggtggatgcc atatcggccg
     4441 cgggtccggc cagcttgagc aatggcaggc acgcggtacc ggcaggcttc tacagccgta
     4501 cggtcgaggg cgcaaagccc cggtcccgat aaactttgta gcgcgcacgc gaggcggcga
     4561 ggatgtcttc gcgggcatcg acgatttcga tcagccgtga acaggcctcg aaaccgtccg
     4621 gcacgtcatc ggcgagattc agcagcagcg cgcgatggct ttccggcggt gcgccggtgc
     4681 cgatcagcac cggccagggc cggcgtccgg gggcgtggat gcgatgggcg atgaagctgc
     4741 cgcggcgcca ggtccagagt tcgcggtcga tcgtcgctga ctgggccgcg gaggcggtgc
     4801 ggatccacac ggacaggccc tgcgaccggg ccttgtcgac cagccggcag gcggtggcga
     4861 ggcaggccgc ctcgccggcg gtggcgagca cgtagaactc gatgcgcgtc gccacggcgg
     4921 cggcaggttc agtcgcgcgc ggcggcagcg cggttgatca ggaagcgggt cagcagcggc
     4981 accggccggc cggtcgcgcc cttctgcttg ccggtcttcc aggcggtgcc ggcgatgtcg
     5041 agatgcgccc agtgcagctt cttcgtgtag cgcgcgagga agcaggcagc cgtgatcgca
     5101 ccgccctcgc gtggaccgac gttggccatg tcggcgaaat tgctgtcgag gccgtcctgg
     5161 tagtcctccc acagcggcag ctcccaggcg cggtcatggg tttcgcggcc ggcgtcgagc
     5221 agctcgttgg cgagcgggcc gtggttcgag aacagcccgt gcggatgcgc gccgagggcg
     5281 atcacgcagg caccggtcag cgtcgcgaca tcgaccagcg tcaccggctc gtagcgctgt
     5341 tcggcccagg tgatcgcatc gcagagcacc agccggcctt cggcgtcggt attgaggatt
     5401 tcgacggtct ggcccgacag gctggtgacg atgtcgccgg ggcgcgaggc ctcgccgccc
     5461 ggcatgttct cgacggccgg caccacggcc acgacgttca gcggcaggtc gagcgcggcg
     5521 atggccgaca tcgtgccgag cacgccggcg gcaccgcaca tgtcgaactt catcaggtcc
     5581 atgtccgggc cgggtttcag cgagatgcca ccggtatcga aggtgatgcc cttgccgacc
     5641 agcacgaccg gcttctgccg ggcatcggct gcgccatgcc attcgagcgc gatcagcttg
     5701 ggcggccggc ggctgccgcg ggcgaccgcg accaggcagc ccatgcccag ggtttccatg
     5761 tcggcgcgct ccagcacgtt cagcgcgaac ttcggagagc tgccggcgag cagcgcggcg
     5821 cgctgggcga ggtattcggg cgtgcagatg ttcggcggca ggttgccgag atcgcgcgca
     5881 agcttcatgc cctcggcgac ggccgcgcct tcgcgcaccg cctgctcgcc gagcggcagc
     5941 tcgcggcggc tcggcacggc gagcacgatg cgcttgagcg gccggcgtgg ggcatccttg
     6001 cggctgcggg tctcgtcgta gcggtagagc acgtcctcgc tgacctcgac ggcgaggcgc
     6061 accttccagg cgacatcgcg cccgcgtacg ttcaggtcgg tcagatagct gacggcttcg
     6121 gtcgcgccgg tgtcgttgag cgtatgcacg gcggcggcga tgatctgctt gtagcggcga
     6181 tcgtcgaggt cgcgctcacg cccgcagccg accagcagca cgcggtcgca gagcgttgcc
     6241 ggcgtgttca cgagcagcag cgtctggccc ggcttgcctt cgaggtcgcc gcggcgcagc
     6301 acgttgccga gaaagccttc ggcagcggtg tcgaactgct cggcgggcgg tgtcaggcgg
     6361 cgggattcgt agacgccgag gacgagacag gcggttctct gcttttcggg attgccgctc
     6421 ttgaccgaga attccatctt tgcgctccga ctctgctggg ggcggccgtg ccggccgtgc
     6481 ttgtgcttcc gctgcgctgc ccgtcgccgg cccggtggcc ggacgggcga ccgagtgtag
     6541 ccggaaggtt tttgaaccga caacgccgct ccggcggctg aggaatcccc gaacttgctg
     6601 cgcacattcg accgattcct gctgcgcgag accgccgcag tcaccgccgc gacgctgctc
     6661 gtgctgctgg cgatcgtgct gtcgagccgg ctcgcgactt acctgaccca ggttgcgagc
     6721 ggtttgctga tcaaggaagt catcctgcag atgctggtgc tgcaggcgat ccgcttcctg
     6781 ccggtgctgg tgccgatcgc gctgatgatc ggcgtgctgc tggtgctggg gcgcctgcat
     6841 caggaaagcg agatggcggc cctgaccggc tgcggctacg gcccggccga gctgtaccgg
     6901 ccgctgctcg cactggcgct gccgctggcg gcgctggtcg cgctggtcgt gctcggcgtg
     6961 gcgccgtggg cggcctcgct gcagttcgag ctgcaggccc gcgcacgtca gcaggcacag
     7021 atgtcattgt tcgtgcccgg taccttccgc gaggcggccg gtggccagca tgtcgtctac
     7081 gtcgaatcgc tcgatccgat cgatggcgaa ctggtcaagg tgttcatcca cagcaccgag
     7141 ccggattcag ggcgcatcgc ggtcacgacc ggcaaccgcg gccatcagga aaccggtgcc
     7201 gacggcgtgc gctacatggt gctggagcgg ggcttccgct acgaaggcga gccgggactc
     7261 accgattacc ggcaggtgca ttacgaacac ctgcgggtgc ggatcgacga gccggcgtcc
     7321 ggggatgtca gccgcaagcg tgaatcggtg ccgacggcgc agttgctgcg cggcgaccgt
     7381 cgcgacaagg ccgaactgca tgcccgcatc ggcgcgatcg tctcgatccc gctgatcgtc
     7441 ttcgtcgcgc cgctgctcgc cagggcccag ccgcgcgaga gccgctatgc gcgcatcctc
     7501 ggcggcgtgc tggtgtacgc gatctacatg aacctgctcg gcatcggcaa ggcgtggctg
     7561 gaaaagggct cgatcgggcc ggtggccgga ctctggtggg cacacctgct gttcctcgcg
     7621 ctcgggctcg cgctgtggtt tcaccactac ggccgccacg gcctgaccgt cagctggaga
     7681 agggcttgaa acgcatcgac gcctacatcc tgcgcacggt gatcggcgcc tcgctgatcg
     7741 ctttgctggt gctggtcgcg ctcgagacct tcttcacgct gctcgtggag ctcgaggatc
     7801 tcggcaccgg ccagtacggc ctcgccgaaa tcctgcgcta tctggcgctg acgctgccgc
     7861 ggcgcaccta cgaaagcttc ccgatggcgc tgctgctcgg tggcctgatg ggcatgggcg
     7921 cgctggccgc cggcagcgaa ctcgtcgtca tgcgcggtgc cggcgtgtcg atcctgcggc
     7981 tggtcggttc ggcgatgcgg gccgggctgc tgctgagcct gttcgcgctg gcgctcggcg
     8041 aattcgtcgc gccgcgaacc gaacgactgg ccgagaccgg tcgcagcacg gccaaggacg
     8101 ccagcgtcac gatccgcgac ggtcgcggct tctgggcccg tgacggtgcg ctgttcgtca
     8161 acgtccgggc cgtgaagccg cacaagcggc tcgccgacat caccttgttc gagatggcgc
     8221 cggattcctc gatgaaggcg gtgatccgcg caccgagcgc cgactacgat ccggcctccc
     8281 ggcgctgggt gctgcgcgac gcccggcgca gcacgctcgg cgaggaacgg gtgacggccg
     8341 atgcgcaggt gtccttcgcc tgggaatcgg tgctcgatcc cggcaagctc gacgtgctcg
     8401 ccgccaagcc cgagacgctg gcgatgcgcc agctgcagac cttcgccggc tatctgcggg
     8461 acaacggcct cagtgccgat cgctacgaac tggcgttctg gtccaaggcc ctggcgccgc
     8521 tcgccaacct gaccatgctg ctgatcgcga tgccgttcgt gttcgcccac cagcgcaatg
     8581 ccgcggccgg gcagcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt
     8701 gaacgtaatc cagtccttcc ggtcctgatt ttccaggagc aggaagtctg caggtgccgc
     8761 tgttgtctcc ggtcatcccg gcgcgaatgc ggcatctgcg cctacagagc gggaccgccg
     8821 cggttcggcg acctgcctgc gatccgccca gggcagggca cccatacatg atcggagcta
     8881 gcatgacgaa gaaccgaacc gatgcggaac cctcgccgtc catagcaatc cacgagacat
     8941 gcccctgtac tggggtcaag agggagaacc ggcgatgacg aagaaccccc cggaaacgtc
     9001 gcgtcgcaat gcattcctgc atgcacatcc gtggttcgtg ccacaggcaa gcgccgccga
     9061 gctcggttcg ggtcagcccg tgctgctgct cgacgacacc tccggcctgc tgcgctcggt
     9121 cagcgccatg ctcaacgccg ccggcgtgcc ggccgtgatc ggtgaaaccg acccgcagcg
     9181 cctgccggcc cggctcagcg aacagggctg cggcgtactg ctggtcgatg cctcgctgcc
     9241 cgagctcgaa cccgaagtcc tgctggcccg cctgagcgcc gagcagccgg acctgccggt
     9301 catcgtgctg accgaggccc atgatcccga ggccgcgatg cgctgcctgc aggccggcgc
     9361 cttcgatttt ctggtcaagc cggtcgatcg cacgcgcctg ctggcgagcg tcggccgggc
     9421 cctggagatc cgcgggctca agcacgagct ggcctcgctg acccagacga tgctcgatga
     9481 ccggctcgat caccccgagg ccttcgcccg gctgattgcg caggaccgca gcatgcgggc
     9541 tgcctgccgc tacatcgagg ccatcgcgcc gctgtcgcag ccggtgctga tcaccggcga
     9601 gaccggcacc ggccgcaagc tgctggccca ggccctgcac gccgtttccg gccgtcccgg
     9661 cgcctgcgtg atggtcgagg tcggcggcat ggacgagcgc cgcttcgccg aaaccctgtt
     9721 cggtcaccgc ccgggcctgc atgccgaagc cggccagccg caggaaggcc gcatcggcgc
     9781 ggcggccgac ggtacgctgg tgctgctcga agtcggtgag cttgccccgg ccgcgcagca
     9841 actgctgctg aacctgctgc aggaacgcag cttcagcccg gtcggctccg gcatcgtgca
     9901 ggagtgccgc gcccgcatcg tcgcgatcag ccgtcatgat ctcgcgcacc aggccgacag
     9961 cggccggctg cgcaaggatc tgtactaccg gctgcgtgcc caccacgtgc acgtgccgcc
    10021 gctgcgcagc cggcgcggcg acgtgccgct gctggtcgag catttcgtcg cgcgcgcggt
    10081 cgaggccctc ggcaagggcg atgtccacat tcctgccgag ctctacagcc tgctgcgcaa
    10141 ccacgacttc cccggcaacg tcgccgaact cgaagccatg gcctacgacg ccgtcgccag
    10201 ccaccgcagc ggcggcacgc tcggcctggc gagcttccgc cactgcgtgt cctccacgcc
    10261 cgatgcgccg ccgccaccac caccggtgca gagtctttcg cagcttttcc ccgaccggct
    10321 gcctacgctg aaggaagccg aagcctttct ggtggccgaa gcgctgcgtc gcgccgacaa
    10381 caatcagggc atcgcggccg gcctgctcgg cctgacgcgt caggcgctga acaagcgact
    10441 ggtgcgcggg cgccagagca gcagccactg aacaaggccc ggcggccgga aataacgatc
    10501 cgcggactgc acgccgtgcc cggcgtgcag gaccgcaggc ttcggggcca tgccccccgt
    10561 gccacccgca ggtgattgct catgctgtcc gccccgctgc cgccagacga agccgagcgc
    10621 ctgtacgcgc tgcgctcgct ggacctgctc gatacgccac gcgaggagcg tttcgagcgc
    10681 atcacccgcc tggccgtgcg cctgttcgga ctgccgatcg cgacggtcag cctgatcgac
    10741 ggtgatcgcg aatggctgaa gtccgtgcag ggactggcgc tgcgcgagat tgcgcgcgag
    10801 cactcgctgg ccgcgcacgc catcctcgac aacgaaccga tgatcgtcag cgacgcccag
    10861 gccgatccgc gcttcggcga ccatccgctg gtactcggcg agccgcgctt ccgcttctac
    10921 gcggcctgtc cgctgcatgc cggccgccgg cgccatcgcg tcggcacgct gagcgtcgcc
    10981 ggccggattg cgcaccggct ggccgaggcc gatgcgcagg cgctgcgcga tctggccgcg
    11041 atcgccgagg ccgagatcgg ccgctcgctg gcgagcgagg tgcagtcgca actgctgcgc
    11101 gaacgcgaca ccgcgcgtcg tcaggcgctg ctcgacgcgc tgaccggcct ctggaaccgc
    11161 aaggccatcc tcgacctgct cgcgcgcgaa ctcgtgcgct gcgagcgcga gggcatcggc
    11221 gtcagcgtcg tgctggtcga tctcgatcac ttcaaggcga tcaacgacaa ccacggccac
    11281 ctgaccggcg atgaggcgct gcgcgccgcc tcggcggcga tgctgcaggc gctgcgcccc
    11341 tacgatgccc tcggccgcta cggtggcgag gaattcctga tcgtcacgac cgacccggag
    11401 ggcggaccgg tcgcggtcgc cgagcgcatc cgcgcgcggc tcgccgaggt gcgcgtcgcc
    11461 accggcagcg gcggtgtcgt ggcgctgagc ggcagcgtcg gcgtcgcgcg ctgcccggcc
    11521 ggcgaacggc gcgagctcga agacctgctg cgcgccgccg accgcgcgct gtacgccgcc
    11581 aagcacgccg ggcgcgaccg cgtcgtcacc gaggacgccg cgaacagcga cgaggccccg
    11641 gttcagcccg acggcagcag caccgacaac gccgcataac gcgcggcctt gccgagcgcg
    11701 atccacagcg tcgccgccgt ccagccgatg ccgagccagc cggcggcgac gcacagcgga
    11761 tcgccgatca ccggcaccca cgaagccagc agggccggtg cgccgaagcg gcgcagccgc
    11821 gtcagcgcct gttcgtggcg ggccgcgaag ggcccggccg ggcgccgctg ccgcagccag
    11881 cggccgatcg caaacgagct gagccccccg agcgtgttgc cgagcgtcgc caccgccagc
    11941 agcgccagcg gcgaatcctc gccgcgcagc agcagcgccg cgtacagcag ttccgagcct
    12001 ccgggcagca gcgtcgcggc caggaagctg ccggcgaaca ggctccagag cccgctgctc
    12061 atcccgtccc ccattccctc actcctcccg ccacgctccc gtcaggcttc gatccaggcc
    12121 ggcgccatcg ccggtcgtgc ctgcagttcg gcgcgcagcc ggcgccagtc cggatcgtgg
    12181 tctgcgacca ccttccagaa cgccggggaa tggttcaggt gcacggtgtg gcagagctcg
    12241 tgcaccagca cgtgccgcac cagcgccggt tcgaggaaca gcagcttgca gttcaggctg
    12301 atgcgaccgc tggccgagca cgagccccag cgcgcccgct gcgtacggat gcgcaccccg
    12361 gcaaagcgca ggccggtttc ctcggcgacg cgtgcaagcc acggcggcag gacttcggcg
    12421 gcgcgccgct gcacgaagcg ctgcagcagc gcgcgggctg cggccgcgtc cgccgcctgc
    12481 aggtgcaggg cgccggcttc gacgagcagc cgggcacgcc gtccttcagg gagggcttcg
    12541 cggcgcagcg accaggtttc gccgagcgcc ggcagcagca tcaccgccgg cagttcgagc
    12601 ccggcgcggc agcgtgcgag ctgcatctcc atccagtggc ggtgcggctc cagcagccgc
    12661 ggcagctcgc gcgccggaaa gccgctcggc acgacgacct cgatgccgtg cgccggcgac
    12721 acgcgcaggc gcacgcgccg ggcgcgggcg tgctcgcgga cccggtacgg gggcagcggc
    12781 ggcagcttca tggctgtggc tcgcgccggc gcaactgcac cgaacgcccg gcctgcgctg
    12841 gcaccggcag ccgttcatgg gcgctgcgca ccgcggcaag ccgtgcgtgc accgccggcg
    12901 gccacggcgc gacccggcgg ctgccgagat cgacgtgcag cagcaggaac tcggcactcg
    12961 ccgccggcca gccggcttcg gcgtggccga gcgtgtggaa caggtgcacg cgcttgtcgt
    13021 cgaggccgag gatctgcgtg tcgactgcga gcggcgcgcc gcccggcagc tcgcgctgat
    13081 agctgacgtg gcattcggcc gcgaacaccg tgctgccggc gctttcgcgc agcgccccgt
    13141 cgagcccgca gcaggcgagg gcggcgtcgg tggcgcggtc gaacaccagc acgtagaagg
    13201 cgacgttcag atggccgttg taatcggtcc agtccgcttc gacggtgcag tgatacggcg
    13261 gcagcggcgc gggcatcgcc ggctcagatc ccgagcgcgc gccagtccgg ccggccgtcg
    13321 agcagcggcg ggcaccagta gtgcgcgccg ttcagcggcc gcgtgaagtt gaacagcgcg
    13381 tcatggatgc cgtcctcggc gccgctcatg cgccgcagga ccgcttcgaa ggcatcgggg
    13441 ctgtggccga aggcgaggaa caccagcccg ccgcggcgtt cggcggtcca tggcatcgag
    13501 cggcgcacca tgaaggccgg cggatcgaag tcctcctgcg cggtgcgctt gacgtgggcc
    13561 gagtccggcg cgtcctcgag ttcctcgttg tcgatgcggc tgcgcccgac cgtgtcgtcc
    13621 tgctcgcccg gcgacaggcg ctggaagcgc tcgatgtcgt gcagccactg ctgcaccgac
    13681 gcgaagctgc cgccatcgag gccgtcgccc tcgtggctga cgatcgcggc ggcgagcgcg
    13741 gcctcgtcga ccgggttctc ggtgccgtcc tcgtaaccgg tcaggtcgcg gctgtcgcgg
    13801 tggcagaagc agtcgatcgc cagttccagc gtgaacacgc cggccagcgc gttctcgatc
    13861 tcgcgcccgg tgtggaacag ctcgccgcgg tcgctgccgc gcagccagca ccacagcgca
    13921 tacggcacgt acgggatcgg cacgcgcgcg ccggggaggc tcgggaacgg ccgcaggccc
    13981 gggatcggcg catcgaggcc ggcggcgagt tcggcgccga tgccgacgac gacggcaaac
    14041 ccgtcgacca gcccgcgcag cgcctgcagc gcccgcggtg cgtcgtgaca ggcgttgacg
    14101 tggaagaaca gatgccgcga ggcggccggg atcggggcga ggatgcctgg ttgcggggta
    14161 ctcatcacgg gggaactccg tcaggacgaa tcggaaggga atcagcagcg cagcagtcga
    14221 ggcagccgct cagggctgcc agcgttccgg gtgcgcccag aacttgctgt tgaaccagtc
    14281 gggcacgctc ttgtagctcg cgatcgcgaa atgcccgagc agcgccgccg gtgcgcgggc
    14341 gcgcagcgtg tagccgaggc cgagcacggc gccctcgtcc cagcacggcc ggggcagcac
    14401 ggccaggcat tccggcgcca gccggtcgcg ggcgcgggcg agcagggcgg ctccggcgcg
    14461 ccggtcgagc agttcgggca ggccgcggat cagcaccgcg tcgtagcggg catcggcggc
    14521 gagcgcgctg gcatcggcac gggcatcgac ctccgtgccc ggtggcagcg tcaggtcgcc
    14581 ggcatcgccg agcagcagca gccggcccgg cgcgcaggcc cgcagccagt cactgcattc
    14641 gttcgcaaac ggcccgcagg ccccggcagg caggtcgatc ggacttatcg gcacatcggc
    14701 atactccgcg cggaaactgg cagtgacggg gacgatcgcg gacactgcgc ggtcacgcga
    14761 gagtacggca gcgccggtcg cgcggtcgat cggcagcgga ggatgcagat gatgcagggc
    14821 aggggcgtga tcgccaccgg caatggtgtg ccgccggtgg atgcgggcct ggcagcgccc
    14881 gatgcggccg tcgcggcctg tccgctgccg gtgctcggca tcgtcggccc gagcggggcc
    14941 ggcaagacgg cactgctctg ccggctgctg ccggagcttg ccgccgccgg tctgcgcgtc
    15001 ggcgtcatca agcaggcctc ggcgcgcttc gatctcgatg agcccggccg cgacagcgat
    15061 cgcctgcgcc gcgccgggct ggcgcgcacg ctggtggccg ccgtcggcca gaccgcgctg
    15121 atcgaggagc gcgacccggc ctgtcagccc ggcgactgcg aactcgccga tgcgctcggc
    15181 tggctcgatg tcggggcgct cgatctggtg ctggtcgagg gcttcgcgcg cgcggcgctg
    15241 ccgaagctcg aagtgctgcg cgccgacggc ccgaagcgcc accagtaccg gcacgatgcg
    15301 tgggtgcgtg cgctgctcgc cgatcatgag cccgagcccg aacctgaatc cgaacccgct
    15361 gccgccgcgg ccgctgcctc ccgcctgcca cggctcgatc tcgccgatgc cacggcgatc
    15421 gccggctggc tgtgcgcgta tcgcgaggaa tttcttgccc gccgcgccgg ccgcaccggc
    15481 ggcgcggccc gatccggaga gctggcccga tgagtctttc ctgtagtccc ggcagcgatg
    15541 ccatccgcgc gcaggcttcg tgctgcgacg atcacgaccc gcactcgctg ccggtgccgg
    15601 tggcgctcgc gcgcatccag accgccgtgc agccggtcac tgccagcgaa caggtggcgc
    15661 tgcgcgccgc gctcgggcgc gtgctcgccg ccgatgtagt ctcgccgatc gacgtgcccg
    15721 cgcacgtgaa ctcggcgatg gacggctacg ccgtgcgcgc cgccgacctg ccggatgaag
    15781 gcaccgtgac gctcgcggtg gtcggcaccg cgttcgccgg tgcggcctgg gacggcaggc
    15841 tcgccagcgg ccaggccgtg cgcatcatga ccggcgccgt gctgcccgag ggtgccgact
    15901 gcgtcgtgat gcaggagcag accgagcgcg agcagggtgg cgatcaggag ggcggccagg
    15961 atcgcgtgcg catcggccgc ggtcaccgcg ccggccagaa catccgcctc gccggcgagg
    16021 acttgaagac cggcgagacc gtgctcgccg ccggccggcg cgtgcagccg gccgatctcg
    16081 gcgtgctcgc ctcgctcggc gtcgccgagg tgcgcgtgcg ccggcgcctg cgcgtcgcct
    16141 tcttctcgac cggcgacgag ctgcgctcga tcggcgagcc gctggccgcc ggctgcgtct
    16201 acgacagcaa ccgctattcg ctgtacggca tgctgagccg cctcggcgtc gagctgatcg
    16261 acatgggtgt cgtgcgcgat gaccgcgagg cgctgcaggc ggccttccgc gaggccgcgg
    16321 cctgcgccga tgccgtgatc acctccggcg gcgtgtcggt cggcgaggcc gacttcgtca
    16381 aggacacgct gctgagcctc gggcgcatcg acttctggaa gctggcgatg aagcccggcc
    16441 ggccgctggc cttcgggcgc ctcggcgagg ccgtgttctt cggcctgccc ggcaacccgg
    16501 tgtcggtggt cgtgaccttc taccagttcg tgcagccggc actgcggcgg atgatgggca
    16561 tgaacgccgt gcagccggtg acgctgcagg tgccgacgct cgcgcgcctg aagaagcgcc
    16621 ccgggcgcac cgagttccag cgcggcgtgc tggagcagcg cgacgacggc tcgatggccg
    16681 tgcgcccgac cggcgaccag ggctcgggca tcctcagttc ggtcagccgc gccgactgct
    16741 tcatcgtgct cgccgccgac agcagcggcg tcgaggccgg cgacacggtg acggtgcagc
    16801 ccttcgaagg gctgttctga tgatgccggc cgccgccgga tgcgcaggcc ggcggccgtg
    16861 gtgagcggtc tcgggctgct cgccgtcggc ctcggcgccg ccgtcggtgc ctggctgcgc
    16921 tggctgctcg ggctgtggct gaacccgctg ttcccgccgg tgccgctcgg cacgctggcg
    16981 gcgaacctga tcggcggcct gctgatgggc atcgcgctcg gcgtgttcca ggcgctgccg
    17041 gcgctggccc cggaatggcg gctggcgctc gcgaccggct tcctcggcgg cctgacgacc
    17101 ttctcgacct tctcggccga gatcgtcacg ctgctcgcac gcggcgaact ggtctggggc
    17161 ggcgcggcga tcctgatgca cgtcggcggc tcgctggccg cgaccgccgt cgggctggcg
    17221 ctggtcggct ggctgcggac cctgggctga gcaagaccgc aaagacgggg agacgatgaa
    17281 gccatgaacc cgagcgaatc cggcatcgcg ctgcgcttcc acctgtccga aggccgacgc
    17341 caccacggcc ggctgctgtg ggaatggctg ctggagctgg cgcgcgagct cgacctgccc
    17401 ggcggcaccg cctaccgggc gctcgccggc tacggccgcc accgtcgcct gcacgaggcg
    17461 catttcttcg agctggccgg cgatgtgccg atcgacgtga tcttcatcct gcgtccggcc
    17521 gacgaggcac ggctgctcga acgcatcgat gccgagggcc tcgcgctctt ctacgcccgc
    17581 atgccgatcg aattcggcct caccggcccc gccgggggct gacctatatt gcagtcgcag
    17641 cattcctgac ggtccggcct gcaccgcgcc cggtgcggac cgctctggct gtgcaagccc
    17701 tccgaccccg agcttccgga aaccgcccgc atgccgaacg atgccgcact ggccgtcgct
    17761 gacgccccgc acaccccgaa gaagaacctg acgacgctga cgctggccgc cctcggcgtc
    17821 gtctacggcg acatcggcac cagtccgctg tacgcgctgc gcgaatgctt cagcggcccg
    17881 cacggcatca tcgcgaccga ggccaacgtg ctcggcgtga tctcgctgat catctggtcg
    17941 ctgctgctgc tggtctcggt caagtacgtc gccttcgtgc tgcgcgccga cttccgcggc
    18001 gagggcggca tcctggcgct gatggcgctc gcctgcagcc ggcgcttcgc gacccgccgc
    18061 cggcactggt ccgtgctgat cctcggcgtg ttcggcgcga cgctgttcta cggcgacgcg
    18121 atgatcacgc cggcgatctc ggtgctgtcg gcgatcgagg gcctcaacgt cgcgaccacg
    18181 gtgttcgagc cctgggtcgt gccgctgacg ctgctggtgc tgatcggcct gttcctcgtg
    18241 cagtcgcacg gcaccgaccg ggtcggcaag ttcttcgggc cggtgatgct ggtctggttc
    18301 ggggcgctgg cggtgttcgg cgtcgccgag atgctcaagc gccccgacat cctcgccgcc
    18361 gtgcatccgc gccacgcgta cttcttcttt cgcgacaacc agtggcatgc cttcgtcacg
    18421 ctcggtgccg tgttcctggt catgaccggc ggcgaggcgc tgtacgccga catgggccac
    18481 ttcgggcgcc ggccgatccg ccgcgcctgg ttcgcgctgg tgctgccggc gatcctgctc
    18541 aactacttcg gtcagggcgc gctgctgctc gccaaccccg cagcggccga gaatccgttc
    18601 tacctgctgg tgccggcgcc gctgctgtac ccgatggtcg ggctcgcgac cgcggcgacc
    18661 gtgatcgcct cgcaggcggt gatttccggc gtgttctcgc tgacgcgcca ggcgatgcag
    18721 ctcggctacg cgccgcgcat gagcatcgag cacacctcgg ccgccgagat cggccagatc
    18781 tacatcccgg cggtgaactg gacgctgctg gtggcggtgc tgctgctcgt gctcggcttc
    18841 gagtcatcga gccggctggc agcggcctac ggcatcgccg tgacgctgac gatggtgatc
    18901 acgaccctgc tgctcgccgt ggtcacgcgc cgggtctggg gctggcatcc ggcgctggtg
    18961 gcggtgtgca tcctgccgtt tctcgccgcg gatctggcct tcctcggcgc caacgcgctg
    19021 aagattccgc agggcggctg gttcccgctg gtggtcagcg cgctgctgtt cacgttcatg
    19081 accacctgga agcgcggccg caagctgctc gcccggcgcc tgcgcgagga ggcgatcccg
    19141 atcaagctgt tcgtcgacga tgccgccgac ggcatgatcc agcgcgtttc cggcacggcg
    19201 atcttcctga cctcgaaccc ggacggcgtg ccgcacacgc tgctgcacaa cctcaagcac
    19261 aacaaggtca tgcacgagcg cgtcgtgctg ctgaccgtgc gcaccgagga agtgccgttc
    19321 gtgcgcgagg acaagcgcgt cgaggtcgag accatcggct ccggcttcta ccgcgtgcac
    19381 atgcgcttcg gcttcaccga ggacccggac gtgcaggcgg cgctggcgct gtgcgggccg
    19441 cacggcctgg agttcgagct gatggagacg accttcttcc tcggccgcga aaccctgatc
    19501 tcgacctcgc gccccggcat ggcgcgctgg cgcgagcggc tgttcatcgc catgtcgcga
    19561 agctcggcgc gggcgatgga cttcttcaac atcccgatca accgcgtcgt ggagctgggc
    19621 acgcagatcg agctgtgagg gcggcgccca cggattccgg ccgcgcagcc gtcgcgggct
    19681 gaccggcaac gcggctggcc gccatgaacg aggcgacccg aaggccgccc cgtgcggtca
    19741 gtgagcgtgc cggctgccgc tccccgttcg gatcgttcgg cccgccgccg ccctcgggca
    19801 cgattccatc gatgttccgg gtcgggtgct ggatgtcgca ggcaagtgcg ggcggggcag
    19861 gactgcttca gtcctcgtcc tccgtttcat cgccagcgtc tgcgtcgatg tcgatgatcg
    19921 cgttggtgcc gcggtagcgg atttgtgcct gcagttcctg cagggcgacc gtcagatcat
    19981 caccagcatg ccgggatgcg agcgaccggg taatcagatc ttcgagacgt tgggcccgcc
    20041 ggcggttggc agggtcgcga ctcgaggggt gcaggaaatc gcggcggcgc atcctgtggc
    20101 gcaggatgaa cagtgccagc cgggcagcgg cacgaaatct caccttgtaa ttgatggcgt
    20161 tgatttcttc ttcgagcacc atcaatgcgg catcggcaag cctgtctgcg gaaatggcat
    20221 tgaggactac accggcggtt tcccggtagg ccagcgcatc gccggctgca cgcagcacga
    20281 aattggaaag cttttttccg tctttcaccg cactgatcga gcagtcagcg caatatcgga
    20341 acaggctgtc gaggtcggct tcggtgcaga gcaccttgcc ggcatgcagc accagggtcg
    20401 ggtcactgac ggtgcctctg gcgatcagcc ggtaataatg cacgatttcg tcggggcagg
    20461 ccgcgtagag cgttgctcca agcctggaca ggttccggcg tgtctggatg tcctgtctgg
    20521 tccgggcatg cttgacggca ctgaagtccg agttgaggca attgcagaat tgctcgatgc
    20581 aaggctgcat ctgcaaggca aattcttcag ggatgtttcc gtctgaatca aaggcagtgc
    20641 aggcggtccg atccgggttg gcgccctgtg tgagaagata tgggctggtg cgcagaccgg
    20701 cgaggctgcg ggtttgaacg aggagttttt tgtactgact gtgcgcagtg gttttcaggg
    20761 ggctggccgt gaattcggtc aaggctttca gaatcgtcag gccgtcaccg cgcttgtatt
    20821 tccagagcag ccgatgcgta cgctgcggtg caatgtccgg atgacccaga ccttgcagtt
    20881 ccagttcgag caggacatct tcccgtgagc cggtgatcgc ggtcattgtg gtccagtcca
    20941 gcaaaatcgc ggactgcccg aggatgccgg atttcgtggg gcgtatctcg acgcgcgcat
    21001 aaccctgagc cggggtctgg gtaacctgca gactgatttc gacggttgtg gatggcggga
    21061 cgggcagcag ggtttcgctg cggcgcgctc cggcttcgtc ctctttcagc aggtagaaga
    21121 ccagcgagcg cgtgtctttg gcgacgatga aacgatcggc cagcgtatgc gtgtaactgc
    21181 tgcccccggc gacgcgctcg gcaggctcga tcagttcggc aaacgcatgc tcgccggcgc
    21241 gcagcacgtt gatttccagc atcggcagga agtcgtaata ggcgatctcg cgggcgccga
    21301 ggcgggtcgc gctgacgctg cagccacggg caacgagggc gtcccgaagt ccggcggtga
    21361 cgatgcgccg gcttgtggaa ctgccgaggg ccgaagtgag gcgcttttgc acgtagtggc
    21421 cgaaggccag cggcgcagcg ccgtccgtgg caagcgcggc atcgttcagc gggccttcga
    21481 tcaggatcag cgcggcgtcc tgcagggccg ccggagtgct cgcgaggact gcatcgaggc
    21541 cgcggcgcag ggcatcggcc agcgcgccgc cgaggtgcgc cggtccctgc aggaggcgcc
    21601 agccgccggg ggcctgcgca tcgggctgca gctcgcgctg ccccgcgcgt ccgagcagga
    21661 cgttccaggc ccaggggcca ctgcagagcg ccggctccgc gcagtccagc tcgcgcgcca
    21721 gcagccggct caggttcccg tgcggccaag gctgccagct gcgcaggcca tcgcgccggc
    21781 gcaccgggac cagcgtcaga cgaccgccct gttcgacggc ttcgaggccg tagctgccca
    21841 gggcgatgcc atcggccagc agctgcatga cgcaggcact gcgaccatgc aaggcctgca
    21901 gcgcccgggc atccagcgct tcgccccagc cgagcaaggc cgcgaccggg cgccacagca
    21961 ggctcaggtc gatgccggcg cgtgccgctc cgtccagcag ccgggcgcgg gtgcgctcat
    22021 caaacccgtc ggtgtccggc accgctgcca cggcggtgcg gacccgcgcg ggcagcaggc
    22081 cggccagatg ccggccgagc agacgggccg cttcgggggc gtcgacttcg tgggcgtcga
    22141 gaggcgcatc gtcgtcgtgc ccgagcgccc gcagcaggct cagcaccggc aggcggatgc
    22201 cgtcttcggc cggccagccg ctgcgcccgg ccggcgcgtc cagcgccatg cggccggtca
    22261 ggacgccgag cggggacgcg tcgatcacca ccggcggtgc ggctgccagc cgcagcgcac
    22321 cgtccggcag cacggtgcag tcacagacgc cgttgagatc gatgccgggc gagaggctca
    22381 tgcgaacagc ctctccaggc tgtcctgcag ggtcagcgcg aactcggcaa acgcctcgcg
    22441 gatgcgcgcc ggatcggccg acagcgtcag gccatagacc cggccggcct gggtggcgaa
    22501 ttccggtgcc gcatcgtgtt tcgagcggat caccagcccg cgggcgcgga tccggtcgtc
    22561 gccatgcagg gcctcccggc gcatcgcctg atccagggct tcgtcggcgg aggctgccag
    22621 cgtttccagg ctcaggggga catcggtgac gccgtgcgcc gcccacacgc agaggctgcg
    22681 ctggcggcca tcgcggccgt cacgcagcac gatgacggcg gtgcagccga gcgcatgctg
    22741 ccacggcgcc acggcggcaa ggccggcggg ctgcgcggcg ggctgcggca ggccgggatc
    22801 gagcacttcg gccaccagcg gcctgccccg cagcgtgagc aggaacacgg ccagccgggc
    22861 gagcgtgtcg gaccagcggc cgcgggcctg ctcgcgccgc agccgcagcg gtgcctgcag
    22921 gcgtacctgc ggatcggcga tgcgtccgag tccgatgacg accgcgagcc gcacgaagtc
    22981 ggcccagagc cgtccctgcc agcgccagag atcatcctcg cgctgctcgg cgctcagcag
    23041 ttcgcgatag gcccgccaga tcacgcgata gcgggcgtcg tgctgcagca cgaaattcgg
    23101 cagcaccggg gcgacggggt cgctgatgcc ggcggcgcgc agctcgcggt ggcagttgcg
    23161 gcacagcgcc tgatagcggc cgacccgccg cgcgcgctcg ctgaggccga agcgctggtt
    23221 ggcgtgctgc cagagtccgg cttcctcgat gctcaggcgc aggaagtcct tcagcacgcg
    23281 attttccagc gtgtcgaggt tttcctcgcg ggcgacgccg agcagacgct ggcgcgggcc
    23341 ggccttctcg gccacggaca ggccgggcct gcggatcatc cattccagac aggcggtatc
    23401 gagttcctgg atgcggtcga tcggcatctg ctggcgcgtg cgcttcagca cgcgccgcgg
    23461 atgtccggcc gtggcttcga ccagcgccgg caggcgtccg gcgtgctcg
//
LOCUS       DAOQIE010000016        22578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1025_length_22578_cov_7.571416, whole genome shotgun sequence.
ACCESSION   DAOQIE010000016 DAOQIE010000000
VERSION     DAOQIE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 22578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22578
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1025_length_22578_cov_7.571416"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="lpxK"
                     /locus_tag="PK144_02025"
     CDS             complement(<1..779)
                     /gene="lpxK"
                     /locus_tag="PK144_02025"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF64532.1"
                     /translation="MLTALRRLAYRCGWLASERLPVPVVVVGNISVGGTGKTPLLAML
                     AEALKARGYRPGLIARGYGGRSTHWPRLVDADSDPLEVGDEPVLLAGLTGCPMAVGPD
                     RPAAARRLLAEHPELDLLLADDGLQHYRLARDLEIAVIDGRRCHGNGWLLPAGPLREP
                     VSRLATVDFVVINGEGGSVQPGEYAMRVEGDTCVNLADPTLTRPLAAFRGRRCLHALA
                     GIGDPQKFFASLEAAGLAFERHPFADHHACRPDELDFGPGTT"
     gene            complement(857..1285)
                     /locus_tag="PK144_02030"
     CDS             complement(857..1285)
                     /locus_tag="PK144_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF64533.1"
                     /translation="MKFRARRSEEPEVMLIPMIDVMLVLLIFFVATTTFVRETGVDLE
                     LPSIDQPVRPHEDRPLVLNIDAGGRYALGDTPLAQQTEAALLAALREAKANAGADTRL
                     IISADRNTPHQYVVTALDLAGKAGLGSIGIAALPKDGPQP"
     gene            complement(1282..1893)
                     /locus_tag="PK144_02035"
     CDS             complement(1282..1893)
                     /locus_tag="PK144_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF64534.1"
                     /translation="MYELVKAGGWVMVPILACSVLALAIILERAWALRRARVLPPSLV
                     ADLRHWAEQGGLQLRTLDERTARSPLGRVVFTALANRHLGREVVKESIEDTGRHIVFE
                     LERFLNTLGTIATIAPLLGLLGTVSGMINILGVVGTHGSGDFKLMASGMSEALVATAS
                     GLLVAIPAVAGYRWLRGRVDELVVGLEKEALLLVDLLQAGRPA"
     gene            2055..3425
                     /locus_tag="PK144_02040"
     CDS             2055..3425
                     /locus_tag="PK144_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNF64535.1"
                     /translation="MSPAGIESGRVSALATRERQRYRDEHPVSARLAREACAHLPGGV
                     PMHWMSDWGLDFPLFLDAAAGAQLRDADGRDYADFCLGDTGAMFGHAPQPVAAMLRAR
                     AGFGYTTMLPGADAIAVAGNLAQRFGLPYWQFATTASDANRFLLRVARAVTGRRKILV
                     FDGCYHGAVDETLVRLGPDGASRPRPGQLGPAFDCAAHTVVVGFDDPDGLDAALAGGD
                     IAVVLTEPALTNAGLVLPQPGFHAHLRAATRRHGTLLAIDETHTISAGPGGCTRAWGL
                     APDFVTLGKPLAGGLPAAAWGLSAGVERALAGVRQPVADGHSGLGTTLSAGALTLAVV
                     RANLEEVMTDAAYARMFALAGQLQHGLERLIGRYRLPWCVVRLGARQELVFRPQPPRS
                     AAESLAAADPALERLLHLYWLNRGVLVTPFHNMLLLSPEHREADLERLLDVLDAALGE
                     LTGAAS"
     gene            3422..4798
                     /locus_tag="PK144_02045"
     CDS             3422..4798
                     /locus_tag="PK144_02045"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNF64536.1"
                     /translation="MNAQNAAGNAGTAAAGEAERFLAAHPDLEAVDLLLTDANGVARG
                     KRLRPHELPPVYAHGRYLPGSILGLDLLGHDVHESGLVWDDGDADRLCVPVPGTLARA
                     PWQPRPTAQALLTMHELDGRPAAGDPRHALVRSVAALEALGLRPVVACELEFYLFDAA
                     SVAGGRPRPVSSRPHATQVYELDALDAVGEFLAELHAHAAVQGVPSETAISEFAPGQY
                     ELTLHHRADALRAADEAILWKRLVKGTAARHGWHASFMAKPLSGHSGSGLHVHVSLAD
                     ADGCNRFASEDPAGTPLLRHAIAGLARAMPDCQLVFAPHANSYRRYRAGSYAPMTPLW
                     GINNRSVGLRVPAGPAPSRHIEHRVAGADANPYLVLAVVLAAVRAGIEAGEEPGPPVR
                     GNGYEQAGERLPASWREAITRAEASGFLREALGADCLRIFTAIKRQEWERFEAEVTDA
                     DWRWGSGL"
     gene            4934..5275
                     /locus_tag="PK144_02050"
     CDS             4934..5275
                     /locus_tag="PK144_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64537.1"
                     /translation="MLSLELVPLGFEYTTPGRARSQAASSATPRSAATNRKSKLDWTE
                     EVEAAIREGRALPLLRPGDYVELRDTCEIGRVERLDGVLAFVRLGDQGSGYSVPTTRS
                     RVRLVAAARSA"
     gene            complement(5344..6168)
                     /locus_tag="PK144_02055"
     CDS             complement(5344..6168)
                     /locus_tag="PK144_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNF64538.1"
                     /translation="MTEFEALTDSELDELDEFLFAQMEAGRQTMLLGAAHGFYTAELC
                     GPQPMPFSDAIDLVLGEAAFESERQHRRLRALLGRLWLEVEAQLDAGEDFEPLLHQIE
                     GDDGQPQTLFADWCLGFIDAVENSEAWQERLASDSPPDEIGSPEQLLEQLPASVTVAL
                     TPIVTLSYRGLWEAQQMVLKEGGELDEQDALDLPAVFRDEVAELTPQQVQTMIDLIGP
                     CVLRLDELNADWRADSDAAAQMPVRVDKIGRNDPCPCGSGRKYKKCCGAAANDPGA"
     gene            6294..9071
                     /locus_tag="PK144_02060"
     CDS             6294..9071
                     /locus_tag="PK144_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNF64539.1"
                     /translation="MNDAPEATAPAARYAVGIDLGTTHTLLAFARLDAAAPAIELFPI
                     AQSVAPGEVAALPMLASMRYHPADGELPAADLALPWGAAAVDAGGTAIVGELARELGA
                     RVPGRLVASAKSWLSHAGIDRTAACLPWGAEPGIAQVSPVDASAATLAHLRAAWDHER
                     PEAPLAAQDIVLTLPASFDETARLLSVEAARRAGLRQLRLVEEPQAACYDWIARHGGD
                     LDARLAGVRLLLVVDVGGGTTDLTLIRVETADGEPPRLTRVAVGEHLMLGGDNMDLML
                     AHVAERRLLADGERLAAGQLAQLIQQCRRAKERLLAVEAPERVRVTLLGSGSRLLGAA
                     RSVEFTREEVREWLVEGFFPVCAADDRPAGRRAALVEFGLPYAADAAITRHLAAFLGR
                     HQAAARSALGEAAPADGLALPDAVLLNGGVFHAPLLVERLLAVLAGWRDGEALRLLEN
                     ADPDRAVARGAVAAALARRGQGLAIGGGAARSYCLLLDAEGSERQAVCLLARGAVEGE
                     EHVLADRAFLLRLGAPVHFALVAGGEERTLAPGELLRVAAADWSALPPVATVLEGSGE
                     VRVQLAASLSEVGTLALACVEEHGPRRWQLEFRLRGEGAEALPVAASASAHPRLDEAA
                     DRILRFYGARAKDVNPKEIKSLRSELEKLLGVRETWDTPLLRELAGVLWEGARRRRRS
                     ADHERLWLSLTGYCLRPGFGWALDDWRVAQVWSLYPLGVQYGGDAQVLAEWWTLWRRV
                     AGGLDAGAQATLLDALLPELAPAARGVKPKAGSDDRVRLAGALERVPAARKLELGTLL
                     LERLQHRHESNQTWWALGRCGARVPFHGSAHAVVPAADAALWCEAALAADWQAAAPAA
                     LAATLLARRSGDRVRDLPEELCRRVGARLRATRQPESWVRMVEAVVELDEADRKRVFG
                     EALPPGLRLAG"
     gene            complement(9107..9778)
                     /locus_tag="PK144_02065"
     CDS             complement(9107..9778)
                     /locus_tag="PK144_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64540.1"
                     /translation="MTFTGSGRLPLALLALLGAASVAAQSLPPGLHGHSPLAAAPPAY
                     VPLSAAGSTDAYFDGTLEQQLFFNAGLLHAPVQAAPAPMSLTSIRPTGGELVNHNEID
                     AALVLTRHGYSSTILPSAGSANPGSGDNYGEYGRFKDLIGKYSGRYENSVRNSIANRN
                     GDDSGSTFASYDGTQDGIDGSGWPKPPAPVVVAPQPGPATALTLPAIRLPGSLHLPKH
                     TISGH"
     gene            complement(9873..10874)
                     /locus_tag="PK144_02070"
     CDS             complement(9873..10874)
                     /locus_tag="PK144_02070"
                     /inference="COORDINATES: protein motif:HMM:NF039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HNF64541.1"
                     /translation="MIDSPWLAERPRLRAAVRVLHFAWPYLAFAIGVASYALIERTDR
                     LAAWLVFTVFASWVWLLAEPWLKPMRGWRLSRRLARYATQAVHQETLFFGLPFLLRAT
                     DWGTGQALFTGAVAILCLVSLIDPLYFRYVCPRRWRFYAFHGLVAFLAMQAFAPVVFR
                     LDTVSGYRLALATGFVVAAQGFIRLRWGWRRWGRIVLLLGAVAALWAIRPWVPPITLW
                     MTSGAITQQFDRRQLRPGEVAVVLSPARLQQGVYAYMALRAPAGLKEPVFHVWRHDGV
                     EFERIPIEITGGREAGYRTWTFKRRFDEAPAGYWTVTAETRGGQRIGRLGFEVRTTP"
     gene            complement(10903..11514)
                     /locus_tag="PK144_02075"
     CDS             complement(10903..11514)
                     /locus_tag="PK144_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNF64542.1"
                     /translation="MTDACLPDGRRLLLASTSPYRRALLERLRLPFACVAPGTDETPR
                     NGESPRGLVARLSLAKAAAVAQREPGALVIGSDQVALLDGAILGKPGTHERAREQLAQ
                     ASGRELHFLTGLCLLDADGGGTQLEVAETVVRFRELLPQQIECYLRREQPYDCAGSFK
                     CEGLGIALFEAILGADPDALVGLPLIALVRMLDAEGVDVLTGF"
     gene            11616..12167
                     /locus_tag="PK144_02080"
     CDS             11616..12167
                     /locus_tag="PK144_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNF64543.1"
                     /translation="MMRALMHARPPDRFDPWKFAVNGGTLAGEWPVATLARFAELLHE
                     PVGSVRWRLAGERDADGRKLVHVWLDAEPRLSCQRCLGPVTLAVQVDALLAPIAAESQ
                     APQLPAQYEPLLAGDEGVSIAELLEDELILALPLVAMHEDERECAAGGYALPPTAGPA
                     PVADGKPNPFAALSTLLKNPKDA"
     gene            12215..12397
                     /gene="rpmF"
                     /locus_tag="PK144_02085"
     CDS             12215..12397
                     /gene="rpmF"
                     /locus_tag="PK144_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNF64544.1"
                     /translation="MAVQQNRKSRSRRDMRRSHDHLEASTLSVDRTSGETHLRHHVSP
                     DGFYRGRRVIAAKAEE"
     gene            12504..13532
                     /gene="plsX"
                     /locus_tag="PK144_02090"
     CDS             12504..13532
                     /gene="plsX"
                     /locus_tag="PK144_02090"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNF64545.1"
                     /translation="MTRPITIALDAMGGDFGPEVVVPAALDFLEARTDLQLILVGRPD
                     VLEARLAACGGLGRAGLSIHAASEVVGMDEPPAQALKHKKDSSMRVAINLVKSGEADA
                     CVSAGNTGALMATARFVLRTLPGIDRPAICTTLPTVHGHVHMLDLGANADCKSDHLLQ
                     FAVMGAELARAVDGLPAPRVGLLNIGSEDIKGNELVKETHALLGNSDLNYIGYVEGDG
                     VYLSPVDVVVCDGFVGNVALKTSEGVAKLVRHFIGEEFRRSLPNRIAGLIAGPVLRAF
                     AKRIDPRRYNGASLLGLNGIVIKSHGGADRLAFANALRIAAIEVEKNVPALIKHHLGA
                     LHAGGDAT"
     gene            13529..14497
                     /locus_tag="PK144_02095"
     CDS             13529..14497
                     /locus_tag="PK144_02095"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNF64546.1"
                     /translation="MKHARIIGTGSYLPERVLDNAALEKLVETSDDWIVERTGVRRRH
                     IAAEHETTADLAEHAARAAIAMAGVDPLSIDLIVVATTTPDHVFPSVATQIQERLGCH
                     GCPAFDVQAVCAGFVFALSVADQFVRTGMSRRALVIGAETFSRILDYRDRGTCILFGD
                     GAGAVLLDAAETPGIIATHLHADGRYKELLWVPNGVSSGYDETQKNRAYVSMRGNEVF
                     KFAVNTLGRVAVETLEKNGIDAAELDWLIPHQANLRIIQATARKLGLPMEKVIVTVAE
                     HGNTSAASVPLALDLGVRDGRIRPGQLLLLEAFGGGFTWGSALVRY"
     gene            14586..15533
                     /gene="fabD"
                     /locus_tag="PK144_02100"
     CDS             14586..15533
                     /gene="fabD"
                     /locus_tag="PK144_02100"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNF64547.1"
                     /translation="MSTSSTLAFAFPGQGSQSVGMLAEAATRPEVRDTFAEAGEALGF
                     DLWQLVQAGPEAELNRTDNTQPAMLAAGVALWRVWCAEGGPQPALLAGHSLGEYTALV
                     AAGALGFADAVRLVAERGRLMQAAVPAGAGAMAALLGLDEPAVREACAEAAQGEVVEA
                     VNLNAPGQIVIAGAATAVARAVEAAKARGAKRAVVLPVSVPSHCALMRPAAERLAERL
                     AAVDLRTPSIPVLHNADVARHAEADAIRAALVRQLHSPVRWIETIEAMAAAGITTVVE
                     CGPGKVLAGMNKRIVKGLAAPTMPDLSALLAVLADQKGA"
     gene            15539..16282
                     /gene="fabG"
                     /locus_tag="PK144_02105"
     CDS             15539..16282
                     /gene="fabG"
                     /locus_tag="PK144_02105"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNF64548.1"
                     /translation="MNLEQRVALVTGASRGIGQAIARELGRRGATVVGTATSESGAAA
                     ISEYLAADGIRGRGLMLNVTDAAAIEAAVKAVTQEFGSLSILVNNAGITRDNLLMRMK
                     DEEWDDIIATNLTSVYRLSKAVIRGMMKARHGRIITVGSVVGSTGNPGQTNYAAAKAG
                     LLGFTKSLAREIGSRGITVNAVAPGFIDTDMTRALPEEARAALVRSIPLERLGRPEDI
                     AHAVAYLASDEAGYVTGETLHVNGGMHMP"
     gene            16448..16690
                     /gene="acpP"
                     /locus_tag="PK144_02110"
     CDS             16448..16690
                     /gene="acpP"
                     /locus_tag="PK144_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF64549.1"
                     /translation="MSTIEERVKKIIMEQLDVKEEDVTSDASFVDDLGADSLDTVELV
                     MALEEEFECEIPDEDAEKITTVQQAIDYVTANAAGN"
     gene            16827..18077
                     /gene="fabF"
                     /locus_tag="PK144_02115"
     CDS             16827..18077
                     /gene="fabF"
                     /locus_tag="PK144_02115"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNF64550.1"
                     /translation="MGTTGKRRVVVTGLGIISPVGNTVDEAWGNILAGRSGIAALTAF
                     DTSNFPVRFGGSVRGFSAEPYIPKKDAKKMDLFIQYGVAAAADALADSGLVVTEANAE
                     RIGVAIGSGIGGLPGIEDGHASFLHGGPRKISPFFVPRSIINMISGQVSILQGLKGPN
                     IAVVTACTTGTHNIGEAARIIAYGDADVMIAGGAEMATTPTGLGGFAAARALSTRNDS
                     PEQASRPWDRDRDGFVLSDGAGVLVLEEYEHAKARGARIYCEFVGFGMSSDAYHMTLP
                     MENGDGARRCMLAALRDARLNPGQIDYINAHGTSTPAGDVAESTAVKAALGEHAYRVA
                     VSSTKSMTGHLLGAAGGVEAVFSVLSLRDQVVPPTINLDQPGEGCDLDYVPHTAREMK
                     IDVALSNSFGFGGTNGTVIFRRLT"
     gene            18162..19502
                     /locus_tag="PK144_02120"
     CDS             18162..19502
                     /locus_tag="PK144_02120"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNF64551.1"
                     /translation="MTCLRELPAVPDPLALHRHDPARYPCLLESVARGGGNGRWSLVF
                     AFPQARLQAQPGTDFLDRLDAAWRAERLPAADDADLPEGLPFRGGWFVYLGYELAAEV
                     EPVLHLPAATDGLPRACALRVPAALAIDHACGRAWAVAEAGGEAHVERLLADCAGLPA
                     VSGPAAEPVLAEALVEDDPAGFLDGVARIREYILAGDVFQVNLSRAWRGRLVPDAGAD
                     ELYARLRVANPAPFAGLARFGAQAVVSSSPERLLRVRGRRIDTRPIAGTRRRDASTSA
                     DAALSAQLLAHPKERAEHVMLIDLERNDLGRVCRPGSVRVDELMAIESYAHVHHIVSN
                     VAGELREDVTPGAAIRAVFPGGTITGCPKVRCMQIIAELEATGRGAYTGAFGYLNRDG
                     DLDLNILIRTLAVDGEHLVLRTGAGIVADSEPAHELAETRHKARGLLLALGAGA"
     gene            19499..20329
                     /gene="pabC"
                     /locus_tag="PK144_02125"
     CDS             19499..20329
                     /gene="pabC"
                     /locus_tag="PK144_02125"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNF64552.1"
                     /translation="MSPDSLIDGVPETRLALADRGLQYGDGVFETLAVRAGRPEFWAP
                     HLSRLAAGCERLGLPLPGAGLLRQEADRLCAARDRAVLKIVLTRGCGGRGYRPPQPCT
                     PTRALSLHPWPADAVPLAPVAVRWCAHPLSENPRLAGLKHLNRLDQVLARAEWDDPDL
                     QEGLMCLPDGTPVEATAANLFLVRDGRLHTPRLDRAGIAGIVRGVLIGRARQLGLEVL
                     EARVDRDELLAADELFLCNSVYGLRPVRALGERRWPAPGPLTQYLAEDFETLRRAEAA
                     "
     gene            20326..21351
                     /gene="mltG"
                     /locus_tag="PK144_02130"
     CDS             20326..21351
                     /gene="mltG"
                     /locus_tag="PK144_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNF64553.1"
                     /translation="MKRILAGALLLVLLLGGGAAWLDYRRFLDSTLPVPAGGLDYLVA
                     PGSSVGALAADLQQRGVLRRALWFELYARLHGLAGRIQAGEYRIEPLDTPRALLDRWV
                     AGRVIVRSFTVPEGWTFAQIRAALATLPKVGQTLAGLDDAQVMERLGEPGLAPEGRFF
                     PDTYRYQSGTPDLELLRQMRRALERQLAAAWARRAPDSPLKTPDEALVLASIVEKETG
                     LPAERRQVAGVYARRLALGMPLQADPTVLYGRGAAADGELTRSDLRSDGPYNTYTRKG
                     LPPTPIAAPGAAALAAAVDPAPGDALYFVARGDGGHVFSATLEDHQRAVRDYRARRAA
                     ASRGESE"
     gene            21348..22025
                     /gene="tmk"
                     /locus_tag="PK144_02135"
     CDS             21348..22025
                     /gene="tmk"
                     /locus_tag="PK144_02135"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNF64554.1"
                     /translation="MNPARGRFIVVDGIEGAGKSTQLDTLRARLEAAGLGVVCTREPG
                     GTPLGEELRELLLRPRAGGIAGDTELLLMFAARAEHIAQVIEPALARGDWVLCDRFTD
                     ASYAYQGGGRGLGFDRVVQLEEFVQRALRPDLVLLYDLPPETGLARAGRRGAADRFEQ
                     EEYEFHARVRLAYINRAAAAPARYRVLDAAAPLAEVSAATLAAIDAFLAEHRPGQGPV
                     TADAAAP"
     gene            22022..>22578
                     /gene="holB"
                     /locus_tag="PK144_02140"
     CDS             22022..>22578
                     /gene="holB"
                     /locus_tag="PK144_02140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNF64555.1"
                     /translation="MTADSSTPASGAGTCEGLPPWLRGIWAQLAPALAAGRLPHALLL
                     AGPEGLGKMAFAQALGEALLCESQRPDHHACGHCRACRLLAAGSHPDLHVVVPAEEGK
                     AIRVDAIRELIGFAALTPQFGGRRVLRLAPAELLNVNAANALLKTLEEPAPGSHLILV
                     SARPARLLPTVRSRCQTIRFAPPPAA"
BASE COUNT         3091 a         8153 c         8176 g         3158 t
ORIGIN      
        1 gtggtcccgg ggccgaagtc gagttcgtcc gggcggcagg cgtgatgatc cgcgaacggg
       61 tggcgttcga aggccagtcc ggcggcttcg aggctcgcga agaacttctg cggatcgccg
      121 atgccggcga gcgcatgcag gcatcgccgt ccgcgaaacg ccgcgagcgg ccgggtcagc
      181 gtcggatcgg cgaggttcac gcaggtgtcg ccttcgacgc gcatcgcgta ttcgccgggc
      241 tgcacgctgc cgccttcgcc gttgatcacg acgaaatcga ccgtcgccag ccgcgagacc
      301 ggctcgcgca gcggcccggc cggcagcagc cagccgttgc cgtggcagcg gcggccgtcg
      361 atgacggcga tctccagatc gcgcgccagc cggtaatgct gcaggccgtc gtcggcgagc
      421 agcaggtcga gttcgggatg ctccgcgagc agccggcgcg ccgccgccgg ccggtccggg
      481 ccgaccgcca tcgggcagcc ggtgaggccc gcaagcagta ccggttcgtc gccgacttcc
      541 agcgggtccg agtccgcatc gaccaggcgc ggccagtgcg tcgagcgccc gccgtaaccg
      601 cgggcgatca ggccgggccg gtagccgcgg gccttgaggg cttcggcgag catcgcgagc
      661 agcggcgtct tgccggtgcc gccgaccgag atattgccga ccacgaccac cggcaccggc
      721 aggcgctcgc tggcgagcca gccgcaccgg taggcgagcc ggcgcagcgc cgtgagcacc
      781 gcgtacaggc cggcgggcgc cagcagcgcg atcgcgagcg ggccgcgccg cgaccagtac
      841 gcgggggcgc gacggctcag ggctgcgggc cgtctttcgg cagcgcggcg atgccgatcg
      901 agccgagccc ggccttgccg gccaggtcga gcgccgtgac cacgtactgg tgcggcgtgt
      961 tgcgatcggc gctgatgatc aggcgcgtgt cggcgccggc gttcgccttc gcttcgcgca
     1021 gcgccgcgag cagcgcggcc tcggtctgct gcgcgagcgg cgtgtcgccg agcgcgtagc
     1081 ggccgccggc gtcgatgttc agcaccagcg gccggtcctc gtgcgggcgc acgggctggt
     1141 cgatgctcgg cagttcgaga tcgacgccgg tctcgcgcac gaaggtcgtg gtcgcgacga
     1201 agaagatcag cagcacgagc atcacgtcga tcatcgggat cagcatgact tcgggttcct
     1261 cgctgcggcg ggcgcggaac ttcatgccgg ccgcccggcc tgcagcagat cgacgagcag
     1321 cagcgcctcc ttttcgaggc cgacgacgag ttcgtcgacg cgcccgcgca gccagcggta
     1381 gccggcgacg gccgggatcg ccaccagcag gccggaggcg gtcgccacca gcgcctcgga
     1441 catgccgctc gccatcagct tgaagtcgcc gctgccgtgc gtgccgacca cgccgaggat
     1501 gttgatcatg cccgagaccg tgccgagcag gccgagcagc ggcgcgatgg tcgcgatcgt
     1561 gccgagcgtg ttcaggaagc gctccagctc gaagacgatg tgccggccgg tgtcctcgat
     1621 cgactccttg accacctcgc ggccgaggtg gcggttggcg agcgccgtga acaccacgcg
     1681 cccgagcggc gagcgcgccg tgcgctcatc gagcgtgcgc agctgcagcc cgccctgctc
     1741 ggcccagtga cgcaggtcgg cgaccagcga cggcggcagc acgcgcgcgc ggcgcagcgc
     1801 ccaggcgcgt tcgaggatga tcgcgagggc gagcaccgag caggcgagga tcggcaccat
     1861 cacccagcca ccggccttga ccagttcgta cacagcgtcc tcccgccccg gcggtccttg
     1921 gaaagcccgg catgataccc gaagcccccg cgacggcagc ggcacgcgcg gctgacagcg
     1981 cgagcgcgcc ggggccggaa cgctgtcccg gcggttctat agtcggaggc ttccttcccg
     2041 tcggagctga aaccttgagt cctgccggca tcgaatccgg gcgcgtgtcc gcgctcgcga
     2101 ccagggagag gcagcgctac agggatgagc accccgtttc cgcccggctc gcccgcgagg
     2161 cctgcgcgca tctgcccggt ggcgtgccga tgcactggat gagcgactgg ggcctggact
     2221 tcccgctgtt cctcgatgcg gccgccggtg cgcagctgcg cgatgccgac ggccgcgact
     2281 acgccgactt ctgtctcggc gacaccggcg cgatgttcgg tcacgcgccg cagccggtcg
     2341 cggcaatgct gcgcgcgcgg gccggcttcg gctacacgac gatgctgccg ggcgccgatg
     2401 cgatcgccgt ggccgggaac ctcgcgcagc gcttcgggct gccgtactgg cagttcgcga
     2461 ccacggcctc ggacgccaac cgcttccttc tgcgcgtcgc gcgcgccgtg accggccgcc
     2521 ggaagattct ggtgttcgac ggctgctacc acggcgccgt cgacgagacg ctggtgcgcc
     2581 tcgggcctga cggtgccagc cggccgcggc ccggccagct cggcccggcc ttcgactgcg
     2641 ccgcgcatac cgtcgtcgtc ggcttcgatg atccggacgg cctcgatgcc gcgctcgcgg
     2701 gcggcgacat cgccgtcgtg ctgaccgagc cggcgctgac caacgccgga ctggtgctgc
     2761 cgcagcccgg cttccacgcg cacctgcgcg ccgcgacccg ccgccacggc acgctgctcg
     2821 cgatcgacga gacgcacacg atcagcgccg gccccggcgg ctgcacgcgt gcctgggggc
     2881 tcgcgccgga tttcgtcacg ctcggcaagc cgctcgccgg cggcctgccg gcggcggcct
     2941 ggggcctgag tgccggggtc gagcgcgcgc tggcaggcgt gcggcagccg gtcgccgacg
     3001 ggcattccgg cctcggcacg acgctgtcgg ccggggcgct gacgctcgcc gtcgtgcgcg
     3061 ccaacctcga ggaggtcatg accgatgccg cctacgcgcg catgttcgcg ctcgccgggc
     3121 agctgcagca cggcctcgaa cgcctgatcg gccgctaccg gctgccgtgg tgcgtggtgc
     3181 gcctcggcgc gcgtcaggag ctggtgttcc ggccgcagcc gccgcgcagc gccgcggaat
     3241 cgctcgccgc cgccgatccg gcgctcgaac ggctgctgca cctgtactgg ctgaaccgcg
     3301 gcgtgctggt gacgcccttc cacaacatgc tgctgctgag tcccgagcac cgggaggccg
     3361 acctcgagcg gctgctcgat gtgctcgatg ccgcactcgg cgaactcacc ggagccgcat
     3421 catgaatgcc cagaatgctg ccgggaatgc cggcacggct gcggccggcg aggccgaacg
     3481 cttcctcgcc gcccaccccg atctcgaagc cgtcgacctg ctgctgaccg atgccaacgg
     3541 cgtggcgcgc ggcaagcgcc tgcgcccgca cgagctgccg ccggtatatg cgcacggccg
     3601 ctacctgccc ggctcgatcc tcggcctcga cctgctcggt cacgacgtgc atgaaagcgg
     3661 tctggtctgg gacgacggcg atgccgaccg gctgtgcgtg ccggtgccgg gcacactggc
     3721 gcgcgcgccg tggcagccgc ggccgaccgc gcaggcgctg ctgaccatgc acgaactcga
     3781 cggccggccg gccgccggtg acccgcgcca tgcgctggtg cgcagcgtcg ccgcgctcga
     3841 agccctgggc ctgaggccgg tggtggcctg cgagctggag ttctacctgt tcgacgccgc
     3901 ctcggtcgcc gggggccggc cgcgcccggt cagcagccgg ccccatgcga cgcaggtcta
     3961 cgagctcgat gcgctcgatg cggtcgggga gttcctcgcc gagctgcacg cgcatgcggc
     4021 cgtgcagggc gtgccgtcgg agaccgcgat ctcggagttc gcgccgggcc agtacgagct
     4081 gacgctgcat caccgcgccg atgcgctgcg cgccgccgac gaggcgatcc tgtggaagcg
     4141 gctggtgaaa ggcacggccg cgcgccacgg ctggcacgcg agcttcatgg ccaagccgct
     4201 gagcggccat tccggcagcg gcctgcacgt gcacgtcagc ctcgccgatg ccgacggctg
     4261 caaccgcttc gcgtccgaag acccggccgg cacgccgctg ctgcgccacg cgatcgccgg
     4321 gctggcgcgg gcgatgccgg actgccagct ggtgttcgcg ccgcacgcca acagctaccg
     4381 ccgctaccgg gccggcagct acgcgccgat gacgccgctc tggggcatca acaaccgttc
     4441 ggtcgggctg cgcgtgccgg ccgggccggc gccgtcacgg cacatcgagc accgcgtcgc
     4501 cggcgccgat gccaatccgt atctggtgct cgccgtcgtg ctggccgcgg tgcgggccgg
     4561 catcgaggcc ggcgaggagc cgggaccgcc ggtccggggc aacggctacg agcaggccgg
     4621 cgagcggctg ccggcgagct ggcgggaggc gatcacgcgc gccgaggcct ccggattcct
     4681 gcgcgaggcg ctcggggcgg actgcctgcg catcttcacg gcgatcaagc gccaggaatg
     4741 ggagcgcttc gaagccgagg tcaccgacgc cgactggcgc tggggatcgg gactctgagt
     4801 tcccgggggg cgcgcatcgt gacacctgtt cacgattgcg cgctccggca ggctggccgg
     4861 cgaacggcgg gcagacctag catgcaccgg tcacggaacc gatcttccca ccccctgcca
     4921 tggagggctg cgcatgctct cgctcgaact cgtaccgctc ggcttcgaat acacgacccc
     4981 gggccgtgcc cgcagccagg cggcttcatc cgccacgccg cgttccgccg caacgaaccg
     5041 caagtcgaag ctcgactgga ccgaagaggt cgaggccgcg atccgcgaag gtcgcgcgct
     5101 gccgctgctg cgccccggcg attacgtcga actgcgggat acctgcgaga tcggccgcgt
     5161 cgagcggctg gacggcgtgc tcgccttcgt gcgcctcggc gaccagggga gcggctattc
     5221 ggtgccgacc acgcgcagcc gcgtgcggct ggtggcggcg gcgcgcagcg cctgagccgt
     5281 cctgccggcc gttcccgggc cgtttcgttc ccgcgctgct tccgcaaacc cctgccgcac
     5341 gcttcaggcg ccggggtcgt tcgcggccgc gccgcagcac ttcttgtact tgcgcccgct
     5401 gccgcagggg catggatcgt tgcggccgat cttgtcgacg cgcaccggca tctgcgccgc
     5461 cgcgtcgctg tcggcgcgcc agtcggcgtt cagctcgtcg agcctgagca cgcacggccc
     5521 gatcaggtcg atcatcgtct gcacctgctg cggcgtgagt tcggcgacct cgtcgcggaa
     5581 aaccgccggc aggtcgagcg cgtcctgctc atcgagttcg ccgccttcct tcagcaccat
     5641 ctgctgcgcc tcccagaggc cgcggtagct gagcgtgacg atcggcgtca gcgcgacggt
     5701 gacgctggcc ggcagctgct ccagcagctg ttcggggctg ccgatctcgt cgggcgggga
     5761 gtcggaggcc agccgttcct gccaggcctc gctgttctcg acggcgtcga tgaagccgag
     5821 gcaccagtcc gcgaacagcg tctgcggctg gccgtcgtcg ccttcgatct gatgcagcag
     5881 cggctcgaag tcctcgccgg catcgagctg ggcctcgact tccagccaca gccggccgag
     5941 cagcgcgcgc aggcgccggt gctggcgttc ggattcgaat gcggcctcgc cgagcacgag
     6001 gtcgatcgca tcggaaaacg gcatcggctg cgggccgcac agctcggccg tgtagaagcc
     6061 gtgcgcggcc ccgagcagca tcgtctggcg gcccgcctcc atctgcgcga acaggaattc
     6121 gtcaagctcg tcgagctcgg agtcggtcag cgcttcaaat tcggtcatgg cggcagtcca
     6181 cgcgcggtga cgacaggggc gcggcatcat cggccgcggc cggagccagg cgcaagcgcg
     6241 ctgcctacaa tgccgccgct ccccagtcgc ccgtccgggc aggattgccg cgcatgaacg
     6301 acgctcccga ggccaccgcg cccgccgccc gctacgccgt cggcatcgat ctcggcacca
     6361 cgcacacgct gctcgccttt gcgcggctcg atgccgccgc gccggcgatc gagctgttcc
     6421 cgatcgcgca gtcggtcgcc cccggcgagg tcgcggcgct gccgatgctc gcctcgatgc
     6481 gctaccaccc ggccgatggc gagctgccgg cggccgatct ggccctgccc tggggcgcgg
     6541 cggccgtcga tgccggtggc acggcgatcg ttggcgagct ggcgcgcgaa ctcggcgccc
     6601 gcgtgccggg ccggctggtg gcgagtgcca agagctggct gtcgcacgcc ggcatcgacc
     6661 gcaccgccgc ctgcctgccc tggggcgcgg agcccggcat cgcgcaggtc tcgccggtcg
     6721 atgccagcgc cgcgacgctc gcgcacctgc gcgccgcctg ggaccacgaa cggcccgaag
     6781 caccgctggc cgcgcaggac atcgtgctga cgctgccggc gtccttcgac gagaccgcgc
     6841 ggctgctgag cgtcgaggcc gcgcgccgcg ccggcctgcg gcagctgcgg ctggtcgagg
     6901 agccgcaggc cgcctgttac gactggatcg cgcgccacgg cggcgatctg gacgcgcgcc
     6961 tcgccggcgt gcgcctgctg ctcgtcgtcg atgtcggcgg cggcaccacc gacctgacgc
     7021 tgatccgcgt cgagaccgcc gacggcgagc cgccgcggct gacgcgcgtc gcggtcggcg
     7081 agcacctgat gctcggcggc gacaacatgg acctgatgct cgcgcacgtc gccgaacggc
     7141 ggctgctcgc cgacggcgaa cggctcgccg ccggtcagct cgcgcagctg atccagcagt
     7201 gccggcgcgc caaggagcgc ctgctggccg tggaggcgcc ggagcgcgtg cgcgtgacgc
     7261 tgctcggcag cggttcgcgg ctgctcggcg cggcgcgctc ggtcgagttc acgcgcgagg
     7321 aggtgcgcga gtggctggtc gagggcttct tcccggtctg cgcggccgat gaccgcccgg
     7381 ccggccggcg cgcggcgctg gtcgagttcg gcctgccgta cgcggccgac gcggcgatca
     7441 cccgccatct ggctgccttc ctcggccggc accaggcggc ggcgcgcagc gcgctcggcg
     7501 aggccgcgcc cgcggacggg ctggcgctgc ccgatgccgt gctgctcaac ggcggcgtgt
     7561 tccacgcgcc gctgctggtc gaacggctgc tggccgtgct cgccggctgg cgcgacggcg
     7621 aggcactgcg gctgctcgaa aacgccgatc cggaccgtgc cgtcgcgcgc ggcgcggtgg
     7681 cggcggcgct ggcgcgccgc ggccagggac tggcgatcgg cggcggcgcg gcgcgcagct
     7741 actgcctgct gctcgatgcc gagggcagcg agcgtcaggc cgtgtgcctg ctcgcgcgcg
     7801 gtgccgtcga gggcgaggag cacgtgctcg ccgaccgcgc cttcctgctg cgcctcggcg
     7861 cgccggtgca tttcgcgctg gtcgccggcg gcgaggagcg cacgctcgcg cccggcgagc
     7921 tgctgagggt cgcggccgcc gactggtcgg cgctgccgcc ggtcgcgacc gtgctcgaag
     7981 gcagcggcga ggtgcgcgtg cagctggcgg ccagcctgtc ggaggtcggc acgctggcgc
     8041 tcgcctgcgt cgaggagcac gggccgcggc gctggcagct cgaattccgg ctgcgcggcg
     8101 agggcgccga ggccctgccg gtggccgcct cggcttcggc gcatccccgg ctcgacgagg
     8161 ccgccgaccg catcctgcgc ttctacggcg cgcgcgcgaa ggacgtgaat ccgaaggaga
     8221 tcaagtcgct gcgcagcgag ctcgaaaagc tgctcggcgt gcgcgagacc tgggacacgc
     8281 cgctgctgcg cgagctcgcc ggcgtgctgt gggagggcgc gcgccgccgc cgccgctcgg
     8341 ccgaccacga gcggctgtgg ctgtcgctga ccggctactg cctgcgtccc ggcttcggct
     8401 gggcgctcga tgactggcgc gtcgcgcagg tctggagcct gtatccgctc ggcgtgcagt
     8461 acggcggcga tgcgcaggtg ctcgccgagt ggtggacgct gtggcgccgc gtcgccggcg
     8521 ggctcgatgc cggcgcgcag gccacgctgc tcgatgcgct gctgccggaa ctggcgccgg
     8581 cggccagagg cgtgaagccg aaggccggca gcgatgatcg cgtgcgcctc gccggggcgc
     8641 tcgaacgcgt gccggcggcg cgcaagctcg aactcggcac gctgctgctc gaacgcctgc
     8701 agcaccggca cgaatccaac cagacctggt gggcgctcgg gcgctgcggt gcgcgcgtgc
     8761 cgtttcatgg cagcgcgcac gccgtggtgc cggccgcgga cgccgccctg tggtgcgagg
     8821 ccgcgctcgc cgccgactgg caggcggcag cgccggccgc gctcgcggcg accctgctcg
     8881 cacgccgctc gggcgatcgc gtgcgcgacc tgccggaaga actctgccgg cgtgtcggcg
     8941 cgcggctgcg cgcgacgcgc cagcccgaga gctgggtgcg catggtcgag gcggtcgtcg
     9001 aactcgacga ggccgaccgc aagcgcgtgt tcggcgaggc gctgccgccg gggctgcggc
     9061 tggccggttg aggcgcgcag cgaatccgtg gccgcgcgcg acgaaatcag tggccggaga
     9121 tggtgtgctt cggcagatgc aggctgccgg gaagccggat ggcgggcagc gtcagcgcgg
     9181 tggccgggcc cggctgcggt gcaacgacca ccggagccgg cggtttcggc cagccactgc
     9241 cgtcgatgcc gtcctgcgtg ccgtcgtagc tggcgaaggt gctgccggag tcgtcgccgt
     9301 tgcggttcgc gatcgaattg cgcacggaat tctcatagcg gccgctgtat ttgccgatca
     9361 ggtccttgaa gcgcccgtat tcgccgtagt tgtcgccact gccaggattc gcgctgccgg
     9421 cggacggcag gatggtgctg ctgtagccat gtcgggtcag gaccagcgcc gcatcgattt
     9481 cattgtgatt gaccagttcg ccgccggtcg gccggatgct ggtcagcgac atcggtgccg
     9541 gggctgcctg caccggggca tgcagcagtc cggcgttgaa aaacaactgc tgttccaggg
     9601 tgccgtcgaa ataggcatcg gtgctgccgg cggcactgag gggtacgtag gccggggggg
     9661 cggccgcgag cggcgaatgt ccgtgcaagc cgggtggcag cgactgggcc gcaaccgaag
     9721 cggcaccgag caaggccagc agggcgaggg gcaggcggcc cgagccggtg aatgtcatgg
     9781 cgatcctcct gctgcacgcc tcggtgcagc ttgcggtcag ggacgatctg ttccggccag
     9841 catagcgccg gctccgtggc ctgccggccg gatcagggcg tcgtgcgcac ctcgaaaccg
     9901 agccggccga tgcgctggcc gccgcgggtt tcggcagtca cggtccagta gccggccgga
     9961 gcctcgtcga accggcgctt gaaggtccag gtccggtaac cggcctcgcg cccgccggtg
    10021 atctcgatcg ggatgcgctc gaactcgacg ccatcgtggc gccagacgtg gaacaccggc
    10081 tccttgaggc cggccggtgc gcgcagcgcc atgtaggcat agacgccctg ctgcagccgg
    10141 gccgggctca gcacgacggc gacctcgccg gggcgcagct gcctgcggtc gaactgctgc
    10201 gtgatcgcgc cgctggtcat ccacagcgtg atcggcggca cccagggccg gatcgcccac
    10261 agcgccgcga ccgcgccgag cagcagcacg atcctgcccc agcgccgcca gccccagcgc
    10321 aggcggatga agccctgcgc ggcgacgacg aagccggtcg caagcgccag ccggtaaccg
    10381 ctgacggtat cgagccggaa caccaccggc gcgaaggcct gcatcgccag gaaggcgacg
    10441 aggccgtgga aggcatagaa gcgccagcgc cgcgggcaca cgtagcggaa gtacagcggg
    10501 tcgatcagcg agaccaggca gaggatcgcc accgcgccgg tgaacagcgc ctgacccgtg
    10561 ccccagtcgg tggcgcgcag caggaagggc aggccgaaga acagggtctc ctgatgcacg
    10621 gcctgcgtcg catagcgcgc cagccgccgc gacagccgcc agccgcgcat cggcttcagc
    10681 cacggctcgg cgagcagcca gacccagctc gcgaacaccg tgaacaccag ccaggcggcg
    10741 agtctgtcgg tgcgctcgat cagcgcgtag ctggcgacgc cgatcgcgaa ggcgagatac
    10801 ggccacgcga agtgcagcac gcgcaccgcg gcgcgcaggc gcgggcgttc ggcgagccac
    10861 ggcgagtcga tcaccggccg gttccggcgg accgcaccgg catcagaagc cggtcagcac
    10921 gtcgacgccc tcggcatcga gcatgcgcac gagcgcgatc agcggcaggc cgaccagcgc
    10981 gtccgggtcg gccccgagga tcgcctcgaa cagcgcgatg ccgagccctt cgcatttgaa
    11041 gctgccggcg cagtcgtagg gctgctcgcg gcgcaggtag cactcgatct gctgcggcag
    11101 cagctcgcga aagcgcacga cggtttcggc gacttcgagc tgcgtgccgc cgccatcggc
    11161 atcgagcagg cacaggccgg tcaggaagtg cagctcccgg ccgctcgcct gcgcgagctg
    11221 ttcgcgggcg cgttcgtgcg tgccgggctt gccgaggatc gcgccgtcga gcagcgccac
    11281 ctgatcggag ccgatcacca gcgcgccggg ctcgcgctgc gcgaccgccg ccgccttggc
    11341 gagcgacagg cgcgcgacca gcccgcgcgg cgattcgccg ttgcgcgggg tttcgtcggt
    11401 gccgggagcg acgcaggcga agggcaggcg caggcgttcg agcagcgcgc ggcggtacgg
    11461 cgaggtcgag gcgagcagca ggcgccggcc gtcgggcagg caggcatcgg tcatcggaat
    11521 caggcctcgc ggcagggcag aggccccagc gtagggagcg gcagggccgt gcggcaacgg
    11581 gccggcgcgt tttgacttgc ccggggtgaa cccatatgat gcgcgccctt atgcatgccc
    11641 gccccccaga ccgcttcgat ccgtggaagt tcgccgtcaa cggcgggacg ctcgcgggcg
    11701 agtggccggt ggcgacgctg gcgcgcttcg cggaactcct gcacgaaccc gttggcagcg
    11761 tgcgctggcg actcgccggc gagcgtgatg ccgatggccg caagctggtg catgtgtggc
    11821 tcgatgccga accccggctg agctgccagc gctgcctggg gccggtgacg ctggccgtgc
    11881 aggtcgatgc cctgctcgcg ccgatcgccg ccgagtcgca ggccccccag ctgccggcgc
    11941 agtacgagcc gctgctcgcc ggcgacgagg gcgtgtcgat cgcggaactg ctggaggatg
    12001 aactgatcct cgcgctgccg ctggtcgcga tgcatgaaga tgaacgcgag tgcgcagccg
    12061 gcggctacgc cttgccgccg actgccgggc cggcaccggt cgcggacggg aaaccgaacc
    12121 cgttcgcggc gctgtcgacc ctgttgaaaa acccgaagga cgcctgagcg tccttccacc
    12181 ggaaacctcc ggcacccaat caggagtccc gatcatggcc gtccagcaga accgcaagtc
    12241 ccgttcgcgt cgcgacatgc gccgctccca cgaccacctc gaagccagca cgctgtcggt
    12301 cgaccgcacc agcggcgaga cccacctgcg tcaccacgtg tccccggacg gcttctatcg
    12361 cggccgtcgc gtgatcgccg ccaaggctga agagtgagac cggccggcac catgccggcc
    12421 cgagcgcaga cgagcctccc tagcggaggc ttcgtcgtta tggccgcacg tgtgcgccgc
    12481 gtgtgtcacg gaaagcagcc gaaatgaccc gaccgatcac gatcgcgctc gacgcgatgg
    12541 gcggcgactt cggacccgag gtcgtcgtcc ccgcagcact cgattttctg gaagcgcgca
    12601 cggatctgca gctgatcctc gtcggccgtc ccgacgtgct cgaagcccgt cttgccgcct
    12661 gcggcggact cggccgtgcc ggcctgagca tccatgccgc gagcgaggtc gtcggcatgg
    12721 acgagccgcc ggcgcaggcg ctgaagcaca agaaggactc ctcgatgcgc gtggcgatca
    12781 acctcgtcaa gagcggcgag gccgacgcct gcgtcagtgc cggcaacacc ggcgcgctga
    12841 tggcgaccgc acgtttcgtg ctgcgcacgc tgcccggcat cgaccggccg gcgatctgca
    12901 cgacgctgcc gaccgtgcac ggtcacgtgc acatgctcga tctcggcgcc aacgccgact
    12961 gcaagtccga tcacctgctg cagttcgcgg tgatgggcgc ggaactcgcc cgcgccgtcg
    13021 acggcctgcc ggcgccgcgc gtcgggctgc tgaacatcgg ctccgaggac atcaagggca
    13081 acgagctggt caaggaaacc cacgcgctgc tcggcaattc cgatctcaac tacatcggct
    13141 acgtcgaggg tgacggcgtc tacctgagtc cggtcgatgt cgtggtctgc gacggcttcg
    13201 tcggcaacgt cgcgctgaag accagcgagg gcgtcgccaa gctggtgcgc cacttcatcg
    13261 gcgaggaatt ccgccgcagc ctgccgaaca ggatcgccgg gctgatcgcc gggccggtat
    13321 tgcgggcctt cgcgaaacgt atcgacccgc gccgctacaa tggtgcaagc ctgctcggcc
    13381 tcaacggcat cgtcatcaag agccatggcg gcgccgaccg gctggccttc gccaatgcgc
    13441 tgcgcatcgc ggcgatcgag gtcgagaaga acgtcccggc gctgatcaag catcacctcg
    13501 gcgccctgca tgccggaggg gacgcgacgt gaagcatgcc cggatcatcg gcaccggcag
    13561 ctacctgccc gagcgcgtgc tcgacaacgc cgcgctcgaa aagctggtcg agaccagcga
    13621 cgactggatc gtcgagcgca ccggcgtgcg ccggcgccac atcgccgccg agcacgagac
    13681 cacggccgat ctcgccgagc atgccgcgcg cgccgcgatc gcgatggccg gtgtcgatcc
    13741 gctgtcgatc gacctgatcg tcgtcgcgac cacgacgccg gatcacgtct tcccgagcgt
    13801 cgcgacccag atccaggagc gcctcggctg ccacggctgc ccggccttcg acgtgcaggc
    13861 ggtctgtgcc ggcttcgtgt tcgccttgtc ggtcgccgac cagttcgtgc gcaccggcat
    13921 gagccggcgg gcgctggtga tcggggccga gaccttctcg cgcatcctcg actaccgcga
    13981 tcgcggcacc tgcatcctgt tcggcgacgg tgccggcgcg gtgctgctcg acgccgccga
    14041 gaccccgggc atcatcgcca cgcacctgca cgccgacggt cgctacaagg aactgctgtg
    14101 ggtgccgaac ggcgtctcca gcggctacga cgagacgcag aagaaccgcg cctacgtgtc
    14161 gatgcgcggc aacgaggtgt tcaagttcgc cgtcaacacg ctcgggcgcg ttgccgtcga
    14221 gaccctcgaa aagaacggca tcgacgcggc cgaactcgac tggctgatcc cgcaccaggc
    14281 gaacctgcgc atcatccagg cgaccgcgcg caagctcggc ctgccgatgg agaaggtcat
    14341 cgtcacggtc gccgagcacg gcaacacctc ggcggcctcg gtgccgctcg cgctcgatct
    14401 cggcgtgcgc gacggccgca tccgccccgg tcagctgctg ctgctcgaag ccttcggcgg
    14461 cggcttcacc tggggttcgg cgctggtccg ctactgagcc gccggccatc catgtccatc
    14521 caggcgtccg ccgctgcccg gcatccggct gcgtgcggcg gcgtcaccag cagggagtcc
    14581 cgcgcatgag cacttcgtcc acgctcgcct tcgcgtttcc ggggcagggt tcgcagtcgg
    14641 tcggcatgct cgccgaagcc gccacccggc ccgaggtgcg cgacaccttc gccgaggccg
    14701 gcgaggcgct cggcttcgat ctctggcagc tggtgcaggc cggccccgag gccgagctga
    14761 accgcaccga caacacgcag ccggcgatgc tggccgccgg cgtggcgctc tggcgcgtct
    14821 ggtgcgccga aggcggtccg cagccggcgc tgctcgccgg tcattcgctc ggcgagtaca
    14881 cagcgctggt cgccgccggc gcgctcggtt tcgccgatgc cgtgcgcctc gtggccgagc
    14941 gcggccggct gatgcaggct gcggtgccgg ccggggccgg cgcgatggcc gcgctgctcg
    15001 ggctcgacga gccggccgtg cgcgaggcct gcgccgaagc ggcacagggc gaggtggtcg
    15061 aggccgtgaa cctcaacgcc cccggccaga tcgtcatcgc cggcgccgcg acagcggtcg
    15121 cacgcgcggt cgaggcggcg aaggcgcgcg gtgccaagcg cgccgtcgtg ctgccggtca
    15181 gcgtgccctc gcactgtgcg ctgatgcgcc cggccgccga gcgtctggcc gaacgtctcg
    15241 ctgcggtcga cctgcgcacg ccgtcgatcc cggtgctgca caacgccgat gtcgcccgcc
    15301 acgccgaagc ggacgcgatc cgcgccgccc tcgtgcgcca gctgcacagc ccggtgcgct
    15361 ggatcgagac gatcgaggcg atggctgccg ccggcatcac gaccgtcgtc gaatgcggcc
    15421 ccggcaaggt gctcgccggc atgaacaagc gcatcgtcaa ggggctcgcc gccccgacca
    15481 tgcccgatct cagcgcactg ctcgcggtgc tcgccgacca gaagggagcc tgaaccccat
    15541 gaacctcgaa cagcgcgtgg cactcgtcac cggcgcgagc cgcggcatcg gccaggccat
    15601 cgcccgcgag ctcggccggc gcggcgcgac cgtcgtcggc accgcgacca gtgaatccgg
    15661 cgccgcggcg atcagcgaat atctcgccgc cgacggcatc cgcggccgcg gcctgatgct
    15721 caacgtcacc gacgccgccg cgatcgaggc ggcggtgaag gccgtcaccc aggagttcgg
    15781 cagcctgtcc atcctcgtca acaatgccgg catcacgcgc gacaacctgc tgatgcgcat
    15841 gaaggacgag gaatgggacg acatcatcgc caccaacctg acctcggtgt accggctcag
    15901 caaggcggtg atccgcggca tgatgaaggc ccgtcacggg cgcatcatca cggtcggttc
    15961 ggtggtcggc agcaccggca atccgggcca gaccaattac gccgccgcca aggccggcct
    16021 gctcggcttc acgaagtcgc tggcgcgcga gatcggttcg cgcggcatca ccgtcaatgc
    16081 ggtcgcgccc ggcttcatcg ataccgacat gacgcgtgca ctgcccgagg aggcccgtgc
    16141 ggcgctggtg cgcagcattc cgctcgaacg gctcggccgg cccgaggaca tcgcgcatgc
    16201 cgtcgcctat ctggcctcgg acgaagccgg ctacgtgacc ggcgaaaccc tgcatgtcaa
    16261 tggcggcatg cacatgccct gagccgtgcc gcaaggcttc ggcctgccgg ttcggtttcg
    16321 tgcaagatca tgatgctgca ggggtttgtc gcgctgcgat tgcacatgac ggcgcttgga
    16381 ccgggcaaat cgtttcactt acaataaccg gcggcatttg ccccgaactt ccgctgcgag
    16441 gaaaccaatg agcaccatcg aagagcgcgt caagaaaatc atcatggagc agctggacgt
    16501 caaggaggaa gacgtcacca gcgacgcctc cttcgtcgat gatctcggcg ccgactccct
    16561 cgataccgtc gaactggtca tggcgctcga agaagaattc gagtgcgaaa tccccgacga
    16621 ggatgccgag aagatcacga cggtgcagca ggccatcgac tacgtgaccg cgaacgccgc
    16681 cggcaactga gctggcgacg ggtcggggca cttgcgccgc ctcggtggcg gcgcggcttc
    16741 tctaccttcc gtaattcctt tcctcaccgc ctgatgcagt tgccggccct gccggcacgc
    16801 ggcgcgggtc gcaagagggc cgctgtttgg gcactacagg caagagacgc gtcgtcgtca
    16861 ccggactggg catcatttcc ccggtcggca acaccgtgga cgaggcgtgg ggcaacatcc
    16921 tcgcggggcg cagcggcatc gcggcgctga ccgcattcga caccagcaac tttccggttc
    16981 gcttcggcgg ttcggtgcgc ggcttcagtg ccgagcccta catcccgaag aaggacgcga
    17041 agaagatgga cctgttcatc cagtacggcg tggcggctgc ggccgatgcg ctggcggatt
    17101 caggtctggt cgtcaccgag gccaacgccg agcgcatcgg cgtcgcgatc ggttcgggca
    17161 tcggcggcct gccgggcatc gaggacggcc acgcgtcctt cctgcacggc ggtccgcgca
    17221 agatctcgcc gttcttcgtg ccgcgcagca tcatcaacat gatctccggc caggtgtcga
    17281 tcctgcaggg cctgaagggt ccgaacatcg cggtcgtcac cgcctgcacc accggcacgc
    17341 acaacatcgg cgaggctgcg cgcatcatcg cctacggcga tgccgacgtg atgatcgccg
    17401 gcggtgccga gatggcgacg acgccgaccg gactcggcgg tttcgcggcc gcgcgtgcgc
    17461 tgtcgacgcg caacgattcg ccggagcagg ccagccggcc ctgggatcgt gaccgcgacg
    17521 gcttcgtgct gtccgacggt gccggcgtgc tggtgctgga ggaatacgaa cacgcgaagg
    17581 cgcgcggtgc gcgcatctac tgcgagttcg tcggcttcgg catgagcagc gacgcctacc
    17641 acatgacgct gccgatggaa aacggcgacg gcgcccgccg ctgcatgctg gcggcgctgc
    17701 gcgatgcgcg gctcaacccc gggcagatcg actacatcaa cgcgcacggc acctcgacgc
    17761 cggccggtga cgtggccgag agcacggcgg tcaaggccgc gctcggcgag catgcgtacc
    17821 gcgtcgcggt cagctcgacg aagtcgatga ccggtcacct gctcggcgcg gccggcggcg
    17881 tcgaggcggt gttctcggtg ctgtcgctgc gcgatcaggt cgtgccgccg acgatcaacc
    17941 tcgaccagcc gggcgagggc tgcgatctcg actacgtgcc gcacacggcg cgcgagatga
    18001 agatcgacgt ggcgctgtcg aactccttcg gtttcggcgg caccaacggc acggtgatct
    18061 tccgccggct cacctgagtc gtcgccgtcc ttcgtctccc ctcgaagccg gttccgggtc
    18121 cgccccgggc cggcttcggc tttgttgcga gtccgcctgc cttgacctgc ctgcgcgaac
    18181 tgcctgccgt gcccgatccg ctggccctgc accggcatga tccggcgcgc tatccctgcc
    18241 tgctggagag cgtcgcgcgc ggcgggggca acggccgctg gtcgctggtg ttcgcgtttc
    18301 cgcaggcccg gctgcaggcg caaccgggca cggattttct cgaccggctc gacgccgcgt
    18361 ggcgggccga gcgcctgccg gctgcggacg atgcggatct tcccgagggc ctgccgtttc
    18421 gcggcggctg gtttgtgtat ctcggctacg aactcgctgc cgaggtcgaa ccggtgctgc
    18481 acctgccggc ggcgaccgat ggcctgccgc gcgcctgtgc gctgcgcgtg ccggcggcgc
    18541 tggcgatcga ccacgcctgc ggccgtgcct gggcggtggc cgaggccggg ggcgaggcgc
    18601 atgtcgagcg gctgctcgcc gactgcgccg gcctgcctgc cgtcagcggg ccggccgcgg
    18661 agccggtgct ggcggaagcc ctcgtcgagg acgatccggc cggctttctc gatggcgttg
    18721 cacgcatccg tgaatacatc ctcgccggcg acgtgttcca ggtgaacctg tcgcgcgcct
    18781 ggcgcgggcg actcgtgccc gatgccgggg ccgacgagct gtacgcgcgc ctgcgcgtgg
    18841 ccaatccggc gccgtttgcg gggctggcgc gcttcggcgc gcaggccgtg gtcagttcct
    18901 cgccggagcg gctgctgcgc gtgcgcggcc ggcgcatcga cacccggccg atcgccggca
    18961 cgcgccggcg cgatgcgagc accagcgcgg atgcggcact gtcggcgcag ctgctcgcgc
    19021 atccgaagga gcgcgcggaa cacgtgatgc tgatcgatct cgaacgcaac gatctcggcc
    19081 gtgtctgccg gccgggcagc gtgcgggtcg acgaactgat ggcgatcgag agctacgcgc
    19141 acgttcatca catcgtctcg aacgtcgccg gcgagctgcg cgaggacgtg acgccgggcg
    19201 cggcgatccg cgcggtgttt ccgggcggca cgatcaccgg ctgcccgaag gtgcgctgca
    19261 tgcagatcat cgccgagctc gaagccaccg gccgcggtgc ctacaccggc gccttcggct
    19321 atctgaaccg cgacggcgac ctcgacctga acatcctgat ccgcacgctc gccgtcgacg
    19381 gcgagcatct ggtgctgcgc accggcgccg gcatcgtcgc cgactccgag ccggcgcacg
    19441 aactcgccga gacccgccac aaggcacgcg gcctgctgct cgcgctcggg gccggggcat
    19501 gagtccggac agcctgatcg acggcgtgcc ggaaacccgg ctcgcgctgg ccgaccgcgg
    19561 cctgcagtac ggcgatggcg tgttcgagac gctggccgtg cgcgccggcc ggcccgagtt
    19621 ctgggcgcct cacctgagcc ggctcgcggc cggctgcgag cgcctcggcc tgccgctgcc
    19681 cggcgccggg ctgctgcgtc aggaagcgga ccggctgtgc gccgcccggg accgggcggt
    19741 gctgaagatc gtgctgacgc gcggctgcgg cgggcgcggc taccggccac cgcagccgtg
    19801 cacgccgacg cgcgcgctga gcctgcatcc gtggccggcc gatgccgtgc cgctcgcgcc
    19861 ggtcgccgtg cgctggtgcg cgcatccgct gtcggaaaac ccgcggctcg ccgggctcaa
    19921 gcacctgaac cggctcgatc aggtgctcgc gcgcgccgag tgggacgatc cggacctcca
    19981 ggaagggctg atgtgcctgc cggacggcac gccggtcgag gccaccgccg ccaatctctt
    20041 cctcgtgcgg gacgggcgcc tgcacacgcc gcggctcgat cgcgccggca tcgccggcat
    20101 cgtgcgcggc gtgctgatcg ggcgggcgcg gcagcttggc ctcgaagtcc tcgaagcgcg
    20161 cgtcgatcgg gacgaactgc tggccgccga cgagctgttc ctctgcaaca gcgtctacgg
    20221 cctgcgcccg gtgcgcgccc tcggcgagcg ccgctggccg gccccggggc cgctgacgca
    20281 gtaccttgcc gaggacttcg agaccctgcg ccgggcggag gcggcatgaa gcgcatcctg
    20341 gcgggcgcac tgctgctggt gctgctgctc ggcggcgggg cggcctggct cgactaccgg
    20401 cgctttctcg attcgacgct gccggtgccg gccggggggc tcgactacct cgtcgctccc
    20461 ggcagctcgg tcggtgcgct cgccgccgac ctgcagcagc gcggcgtgct gcgccgggcg
    20521 ctgtggttcg agctgtacgc gcgcctgcac gggctggccg ggcgcatcca ggccggcgag
    20581 taccgcatcg agccgctgga cacgccgcgc gcgctgctcg atcgctgggt cgccgggcgc
    20641 gtgatcgtgc gcagcttcac ggtgcccgag ggctggacct tcgcgcagat ccgtgccgca
    20701 ctcgcgacgc tgccgaaggt cgggcagacg ctggccggcc tcgacgatgc acaggtcatg
    20761 gagcgtctcg gcgagccggg gctggctccg gaaggccgct tcttcccgga tacctaccgc
    20821 taccagagcg gcacgcccga tctggagctg ttgcggcaga tgcgccgggc gctggagcgc
    20881 cagctcgccg ccgcctgggc gaggcgcgcg cccgattcgc cgctgaagac gccggacgag
    20941 gcgctggtgc tggcctcgat cgtcgagaag gaaaccggcc tgcccgcgga acgccggcag
    21001 gtggccggcg tctacgcgcg gcggctcgcg ctcggcatgc cgctgcaggc cgacccgacc
    21061 gtgctgtacg ggcgtggagc cgcggcggac ggggagctga cgcgcagcga tctgcgcagc
    21121 gacggcccgt acaacaccta tacccgcaag ggcctgccgc cgacgccgat cgccgccccc
    21181 ggtgctgcgg cgctggccgc ggccgtggac ccggcccccg gcgatgcgct gtacttcgtt
    21241 gcccgcggcg atggcgggca cgtgttttcc gccacgctcg aagaccacca gcgcgccgtg
    21301 cgcgactacc gcgcgcggcg tgccgccgcc agccgaggag aatccgagtg aatccggcca
    21361 gaggacgctt catcgtcgtc gatggcatcg agggtgccgg caagtccacg cagctcgaca
    21421 ccctgcgcgc acggctcgaa gccgccggcc tcggcgtggt ctgcacgcgc gaacccggcg
    21481 gcacgccgct cggcgaggag ctgcgcgagc tgctgctgcg cccgcgcgcc ggcggcatcg
    21541 ccggcgatac cgaactgctg ctgatgttcg cggcgcgcgc cgagcacatc gcgcaggtca
    21601 tcgagccggc gctcgcgcgc ggcgactggg tgctgtgcga ccgcttcacc gacgcgagct
    21661 acgcctacca gggcggcggc cgcggcctcg gcttcgatcg cgtcgtgcag ctggaggaat
    21721 tcgtgcagcg ggcactgcgg cccgatctgg tgctgctcta cgacctgccg cccgagaccg
    21781 gtcttgcccg cgccggccgc cgcggtgcgg ccgaccgctt cgagcaggag gaatatgaat
    21841 tccacgcgcg cgtgcggctt gcctacatca accgggcggc ggccgcgccg gcgcgctacc
    21901 gcgtgctcga tgccgctgcg ccgctcgccg aggtcagcgc cgcgacgctg gccgcgatcg
    21961 acgccttcct cgccgagcac cggcccggac aagggccggt cacggcggac gccgccgcgc
    22021 catgacggcg gactcctcca cgcctgcatc cggcgccggc acctgcgaag gtctgccgcc
    22081 gtggctgcgc gggatctggg cgcagctcgc cccggcgctg gccgcgggcc ggctgccgca
    22141 cgcgctgctg ctcgccggcc ccgaagggct cggcaagatg gcgttcgcgc aggcgctcgg
    22201 agaagccctg ctctgcgaaa gccagcgtcc cgaccatcac gcctgcggtc attgccgcgc
    22261 ctgccggctg ctcgccgccg gcagccatcc ggacctgcac gtggtcgtgc cggccgagga
    22321 gggcaaggcg atccgcgtcg acgcgatccg cgagctgatc ggcttcgcgg cgctgacgcc
    22381 gcagttcggc ggccggcgcg tgctgcggct ggcgccggcg gagctgctga acgtcaacgc
    22441 cgccaacgcg ctgctgaaga cgctggagga accggccccc ggcagtcacc tgatcctggt
    22501 cagcgcccgg ccggcgcggc tgctgccgac cgtgcgcagc cgctgccaga cgatccgctt
    22561 cgcaccgccg ccggccgc
//
LOCUS       DAOQIE010000017        21975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1064_length_21975_cov_7.362682, whole genome shotgun sequence.
ACCESSION   DAOQIE010000017 DAOQIE010000000
VERSION     DAOQIE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21975
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_21975_cov_7.362682"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK144_02145"
     CDS             <1..598
                     /locus_tag="PK144_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNF64556.1"
                     /translation="TSPAAGTASVEVLRGNGLRDRRELQVRIRQAVLAARESVLITTP
                     YPLLSRRLLRTLRRRVADGIEVRMLIPEHSDVYAVDLARTRLIRQLVRQGVRVYRYRR
                     PMHTKTLVVDDAFALVGSFNLDIWGSAHNLEIGLAIHDTGAAARLAADFRADLTDALS
                     DPPPPSPLPRRALEWLAQRALRIGLLHATRPGREGAGN"
     gene            654..911
                     /locus_tag="PK144_02150"
     CDS             654..911
                     /locus_tag="PK144_02150"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNF64557.1"
                     /translation="MATKKAPATDIESALKELESLVEALEQGELTLEDALARYERGVS
                     LARTCQGLLGAAEQRIELLQGQAGAESLVPWTPPAAAAPQD"
     gene            920..1807
                     /gene="ispA"
                     /locus_tag="PK144_02155"
     CDS             920..1807
                     /gene="ispA"
                     /locus_tag="PK144_02155"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HNF64558.1"
                     /translation="MPEARIEAYRARAELALDARLPAAATLPARLHEAMRYAVLGGGK
                     RVRPVLVYAAGEAVGAAPEGLDGPASALEFIHAYSLVHDDLPAMDDDDLRRGRPTCHR
                     AFDEATAILAGDALQTLAFHVLAHDPSMTVPADTRLRMVELLAHAAGSRGMCGGQAID
                     MAAVGRELTLAELENMHIHKTGALIRAAVLLGALSQPGVADEELARLDRYAKCIGLAF
                     QIHDDILDVVGDTATLGKRQGADQALDKPTYPALLGLDGARAHARALYDDAMHALEPF
                     GERAAVLRALAAFIVERSH"
     gene            1849..3717
                     /gene="dxs"
                     /locus_tag="PK144_02160"
     CDS             1849..3717
                     /gene="dxs"
                     /locus_tag="PK144_02160"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNF64559.1"
                     /translation="MTALTPVFPLLDTIDSPQDLRRLPENVLGQLANELREFLIQSVA
                     RTGGHFSANLGTVELTIALHYVYDTPEDRLVWDVGHQSYPHKILTGRRALMGTMRRRH
                     GLAGFPKRCESPYDTFGVGHSSTSISAAQGMAIAAEAAGSRRRAVAVIGDGAMTAGMV
                     FEALNHAGHLKNDILVVLNDNEMSISPNVGALSSYLTRLLSGHVYSTVREGGKRILAH
                     LPSSMWEMARRAEEHAKGLVTPGTLFEELGFNYFGPIDGHDLPTLVRTLENLRDQKGP
                     RLLHVMTRKGKGYKLAENEPVTYHGVGSFDPASGIPPAKTGGGPTYTQVFGEWLCDMA
                     AQDPRLVGITPAMREGSGLVEFAERYPTRYFDVGIAEQHAVTLAAGMACDGLKPVVAI
                     YSTFLQRAYDQLIHDVAIQNLPVLFAIDRAGLVGPDGPTHAGSFDLCYLRCVPNLLIM
                     APADEDECRQMLYTGFLHDGPACVRYPRGKGPGVAPVATMTALPIGRAELRRQGRGTA
                     LLAFGAMLPLAEAVGERLDATVVNMRFVKPLDTAMVLELARTHERLVTIEDGAVMGGA
                     GSAVAECLAGHGVHTALKLLGLPDRFLDHGERGELFAECGLDIDGVLAALAAQSDH"
     gene            3904..4728
                     /gene="folE2"
                     /locus_tag="PK144_02165"
     CDS             3904..4728
                     /gene="folE2"
                     /locus_tag="PK144_02165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNF64560.1"
                     /translation="MPTAVPAPQQQQPAAIPDIQSTPDTRRLPIDKVGVKDLRHPVRV
                     RDRSDGEQHTVATFNMYVRLPHDFKGTHMSRFVEVLNRPGREIGVASFHEMLTEMNAR
                     LDAGTGHIEMSFPYFVMKTAPVSGVQSLLDYQVTLIGELAGEQATTWLRVVVPVTSLC
                     PCSKEISRYGAHNQRSHVTVSARLSGFVWIEELIDIVEQEASCELYGLLKRPDEKYVT
                     ERAYENPKFVEDMVRDVAARLNDDDRIAAYVIESENFESIHNHSAYALIERDKTAG"
     gene            complement(4811..7231)
                     /locus_tag="PK144_02170"
     CDS             complement(4811..7231)
                     /locus_tag="PK144_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNF64561.1"
                     /translation="MAPGVAAAIGVGPIQVQSGLGQRLRAQVELLSVAPDELQNLSVR
                     LAPPQAFAQAGLDRTGALSDLQFAVEAQPGGRSVIRITSQRPVREPALNFLVELGSPR
                     GKLMREFAILLDPVRPNERRATAASPIGGYAGQPSAGGGTYGPIRPGETLWSIADRLR
                     PADMSNREAVRRLYDANPEAFSRRSPDGLRAGAMLRMPAESGWQGNPPAPAVPAPAQP
                     LPVTPPAVATRPPASPAPATAKPPEMPPAGAQVRLLPPEGQSGGNALPAPGMGMTDQP
                     RGPDPVAGGGYGQIELDNGRLRLQAAGLDHLQNRIDTLPPAERMPVPVTPPVAEPPRP
                     ASVTPTQPTPAPAPAPAPVAARPPVKPAPEPDFLDSVLLAFEDPLVLAGLGVAVAALL
                     GGGLLAMRRARRAQDAEHEMFESITLAEDHAAPGAAPLPAAAGAAAASADPLERSSLL
                     MSLGNHAEARALLESALAAAPDNTALRMRLLEVLHTLRDAAAFRREVGPLRQLVYGDT
                     DPMWLRVARMGRELCPDDPMFIGTVAATPPGMPATSPRMPVTPMPAPASAAAAAPARV
                     DDEVWSDLDFKPMDMPEAAPAATMAPKAPAVAPAKLPPAAVVPPGPSLDFEPVEVRRP
                     AEKPAPVPVPAPATTPEDLLADLGWSDADLGSPPAMPEKPVTAAPAPRPAEVADLDFG
                     DDLAAFLPPRQTSTPAQAAAKPAAVDPATAELADDLGFSFQPVSAPAPAPLPVVPAPA
                     AAPPVSASTADTRGEDEFVETKLDLALAYIDMDDPLGAKSLLEEVLQEGGSSQRSRAQ
                     ALLERLAS"
     gene            complement(7394..8401)
                     /locus_tag="PK144_02175"
     CDS             complement(7394..8401)
                     /locus_tag="PK144_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNF64562.1"
                     /translation="MSHDHPAPVIAVLGADGLLGEAMLAAFAADGLPAAQLIALGGED
                     VEGERLAYGDAGLRVQPAAGCDWSRIALVIATDDAALAAAQLPLARRAGARVLDASGW
                     LAGEAPAPVAIAGHDENRWRRADALVLPDAVTLALARVLMPLAALAGIGHVGLTVLRA
                     ASEDGRAAVEELAQQTIALLNARPSNPQIYPRRLAFNVLPEWQDLDAQGRGGGERRLD
                     AGLEQVLGLAPGSVATTLLRVPLFYGLACVVHLRCTAALSPAAARERLLEVPGLEVLE
                     ADEAPVSPAEDGLGQPAVRVGGLRAAGERGLNLWVVADNVALPASNGVQIAQTLIGTA
                     E"
     gene            complement(8493..9602)
                     /gene="asd"
                     /locus_tag="PK144_02180"
     CDS             complement(8493..9602)
                     /gene="asd"
                     /locus_tag="PK144_02180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNF64563.1"
                     /translation="MRRVGLVGWRGMVGSVLMERMRAENDFALIDPVFFTTSNVGGSG
                     PDIGKPIAPLADARSVEALTAMDVIISCQGGDYTNEIYPQLRAAGWDGYWIDAASALR
                     MEPDAVIILDPVNRKVIDAALAQGVRNYIGGNCTNSILLMGVGGLFEAGLVEWVSSMT
                     YQAASGAGAQNMRELLTQMGVVNGAVSGLLADPASAILDIDRQVAATMRSSDFPTQHF
                     GAPLAGSLIPWIDKQLDNGQSKEEWKGQAEVNKILGTRETIPVDGLCVRIGAMRCHSL
                     ALTLKLTRDLPLAEIEALIRGSNPWVKFVPNERPLTVQELTPTAVTGKLDIAVGRVRK
                     LNMGPEYVSAFVCGDQLLWGAAEPLRRMLRILLEA"
     gene            complement(9719..10795)
                     /gene="leuB"
                     /locus_tag="PK144_02185"
     CDS             complement(9719..10795)
                     /gene="leuB"
                     /locus_tag="PK144_02185"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNF64564.1"
                     /translation="MTKKILVLAGDGIGPEIVGEAVRVLEVLRSGYGLDVELDTALLG
                     GCAVDATGEPYPDATRAKAQAADAVLLGAVGGPQYDTLPRPQRPERGLLAIRKDLNLF
                     ANLRPAVVYPQLAAASSLKPELVAGLDILIVRELTGDIYFGEPRGIELRDGERVGWNT
                     MIYSESEIRRIVRVAFEAARKRGGRVCSVDKMNVLECTQLWRDVAEEVAKDYPDVQLT
                     HMLVDNAAMQLVRNPKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDAHGKGLY
                     EPIHGSAPDIAGKGVANPLATILSVAMMLRYSLGRADLAERVERAVSRVLDQGLRTAD
                     IRAEGCETVGTVAMGEAVLAALAD"
     gene            complement(10843..11394)
                     /locus_tag="PK144_02190"
     CDS             complement(10843..11394)
                     /locus_tag="PK144_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64565.1"
                     /translation="MSSIEMAVRNARAIETLLVERLGANGKGLHEKLNSVERQLPPHI
                     VKTARWIATLRNSAVHQHDFEISNPDDFMNAAARVLAHLESMPKTAGAAAAAQAAESS
                     RSGSMSGGLRLETVRASRPQPGAHQGGSAPAAVPARTARRRRQPEARSSGQGWWLAFV
                     LVAGGLLVAALFKSGALRSIVSG"
     gene            complement(11508..11897)
                     /locus_tag="PK144_02195"
     CDS             complement(11508..11897)
                     /locus_tag="PK144_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF64566.1"
                     /translation="MRLLLDTHALLWWFAGDERLPEKVRSFIADRQNAVLVSAASIWE
                     IATKHRLGKLEGVGELLADIDTYVRDQSFDWLDIAAVHALRAGLLPGAHRDPFDRMLI
                     AQALCVEAVLVSNETLFDGFGVQRLWN"
     gene            complement(11897..12133)
                     /locus_tag="PK144_02200"
     CDS             complement(11897..12133)
                     /locus_tag="PK144_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017926937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNF64567.1"
                     /translation="METCPVHEAGEHLAVLIERAWRGEDIIITGEAAQAVKLVPVTTR
                     AAGQRQFGRLRGRIRVDDAFFDPLPEEVLAAWER"
     gene            complement(12177..12815)
                     /gene="leuD"
                     /locus_tag="PK144_02205"
     CDS             complement(12177..12815)
                     /gene="leuD"
                     /locus_tag="PK144_02205"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNF64568.1"
                     /translation="MQPFTELHGLVVPLDRPNVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDVGEPGQDCAGRPRNPDFSLNQPRYAGASVLLARENFGCGSSREHAVWALEDY
                     GIRCVIAPSFADIFFSNCGKNGVLAIRLPAETVDRLFRAVEATPGYQLFVNLPGQTIA
                     TPDGEVIDFDIDAFRKECLVNGWDDIGLTLRHAGDIRAYEAKRRQEAPWLFQ"
     gene            complement(12840..14252)
                     /gene="leuC"
                     /locus_tag="PK144_02210"
     CDS             complement(12840..14252)
                     /gene="leuC"
                     /locus_tag="PK144_02210"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNF64569.1"
                     /translation="MSGQTLYDKIWAAHVVRTDADGTALLYIDRHLVHEVTSPQAFEG
                     LKLAGRAPWRKASMLAVPDHNVPTTPRAGGIGDIEDPVSRTQVETLDANARELGLAYF
                     PINDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQ
                     CLIQKKAKAMLVRVDGEAGPGVTAKDIVLAIIGRIGTAGGTGYAIEFGGSAIRALSME
                     GRMTVCNMAIEAGARAGMVAVDATTLGYVQGRPYAPKGADWDKAVAWWNTLHSDADAR
                     FDAVVELDAAAIAPQVTWGTSPEMVVAVGENVPDPAAETDTVKKSGMEKALAYMGLAA
                     RQPVTDIRLDRVFIGSCTNSRIEDLRAAAAVIRGRKVAASVKQALVVPGSGLVKQQAE
                     AEGLDKLFLDAGFEWREPGCSMCLAMNADRVGPGEHCASTSNRNFEGRQGAGGRTHLV
                     SPAMAAAAAIAGHFVDVRSF"
     gene            14381..15289
                     /locus_tag="PK144_02215"
     CDS             14381..15289
                     /locus_tag="PK144_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF64570.1"
                     /translation="MDIASLQAFVAVADSGSFSVAAERLFLTQPAVSKRISALEAELG
                     VRLFDRLGRRVLVTEAGGTLLPRARRILAELDDSRRALADLSGQVAGRLSVATSHHVG
                     LHRLPPVLRAYTASYPQVQLDLRFMASEAACLAVAGGDVELAIVTLPLAAEAPLALQT
                     IWTDRLVLVAHPGHPLARLKRVSPFRLVEHAAILPEHGTFTRELIERCFEPLGIRLRV
                     AFTTNFLETIKMMVSIGLGWSLLPRTMLDAQLAVLELEGIAPTRALGLVAHAGHTPSN
                     AARALLGLLAPGEGGGPAEPEPWRGY"
     gene            15375..15995
                     /gene="hflD"
                     /locus_tag="PK144_02220"
     CDS             15375..15995
                     /gene="hflD"
                     /locus_tag="PK144_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HNF64571.1"
                     /translation="MNKTDRERTIALAAIFQAAALVRDVAYAGQADAADSEACLRSIF
                     AIDSPGIAEVYGGLPGLRRGLRLLASELRTPREMEIARYTVNLLVLERKLRASPAMTE
                     RLRQGIRDTARRLDHFPVTHENIVAALGELYADTISTLTPRIMVNGEQMHLSRRDNAA
                     RIRALLLAGIRAAWLWAQAGGGRLTLLLRRRALLAESQRLLRELGD"
     gene            16275..17366
                     /locus_tag="PK144_02225"
     CDS             16275..17366
                     /locus_tag="PK144_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64572.1"
                     /translation="MDQPSLPAAGAGHLPPDPLNASLPETAGMPAVETAILNWLWRRE
                     HRIADTPARLMVALRDGALPELCAASLRRHFGRLDAAAAPPPELRLHDPVSPQLPQAD
                     ILIGPQHALIDALPAAEARQAQWLLDAPPVPVARRACQRLAPAFAAGRVRVLPATGCE
                     ADAVLPPLPLHAATALLDAHRPGTTTLGVPATLAQVRSILRELQQLAPGTALVAVHPC
                     FRSAHRAALAASLLNAPAGGRILVATPAVAAQLQADRRLVALDGGEAATGGHEAPVLN
                     VLWRLRGSEAAPQSAELCPAPPVAVRKLRRERWLWDRAAGRWQHCQPSTALPAGSIVR
                     LNAEEGGYDATLGWMPHARLTVVPALTVD"
     gene            complement(17394..19547)
                     /locus_tag="PK144_02230"
     CDS             complement(17394..19547)
                     /locus_tag="PK144_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF64573.1"
                     /translation="MNRRKLLFAFGFIALIAGFFASGAGEWLSLAALKARHGELLAWR
                     DAQPLTAALGFAGLYVAVTALSVPGATVLTLAAGALFGLGTGTLLVAIAATTGATLAM
                     LVARFLLRDAIQARFGERIRALDAGIERDGAFYLFTLRLVPVVPFFLINLASGLTALP
                     VRTYVWVSLIGMLPGTVVYVNAGTQLARVESLSGLLSPGLIASFVLLGVFPLLARRAL
                     DAWQAHQVYRPWPKPKRFDRNLIVIGAGSAGLVSAYIAAAVQAKVTLVERAEMGGDCL
                     NRGCVPSKALLRTARFLAEQRRAPDYGIGEASARFDFAEVMARVQRVIAAVAPHDSVE
                     RYTGLGVEVLAGTARLLSPWTVAITAADGSERRLDTRAIVIATGARPFVPPIPGLEEG
                     GYLSSDTLWALRECPQRLLVLGGGPIGCELAQAFARLGARVTQVEMLPRLLAREDADV
                     SAHIGAVFAAEGIDVRCGHRALRCERDAGGGRVLVAEHAGTEVRLGFDAILVALGRAP
                     VTDGLGLEALGIGLTKSRTIETDAFLQTRFPNVLVCGDVAGPYQFTHTAAHQAWYAAV
                     NGLFGQFRRFRADYSVIPWATFTEPEIARVGLNEQEARERGIAFESSVYRLDDSDRAL
                     AESEPQGWVRVLTVPGRDRILGATIVSAHAGETIAGFVLAMRHGLGLEKLLATIHVYP
                     TWAESGKAVAGTWKRAHAPQRLLGWVRRYHDWRRG"
     gene            19906..20316
                     /locus_tag="PK144_02235"
     CDS             19906..20316
                     /locus_tag="PK144_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64574.1"
                     /translation="MDFRSRYAAFLDELDAIAETHDELFDTEVRERLREVIDYHFVWD
                     QPIGEDFPRVFAMFSDTADALVAAAVRSFIEEACALARAEGISTAAARHAAIEDDTLL
                     GDEGGFGDYLVDTELTDAPVPPASDALYLSDARP"
     gene            20303..21304
                     /locus_tag="PK144_02240"
     CDS             20303..21304
                     /locus_tag="PK144_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNF64575.1"
                     /translation="MPGPDRPSPAASRGRSARRPGRALLLCFALALGGCSSLAWLRLP
                     WSLTHAGHGLLAWFVPLAVLSVLLARGLQQWLRGPKVSAAGPQPLLTTYEAAFLAGGE
                     ARVVEVACASLAQRGWLITTQDWQALKPTKICGTPEDPLERAVLAAIARDGRPAHLRR
                     VAALRIAPALYRRLHALGLLHDARTLQRLRRWPALLTGTVLLAGLAGIGFGLLHQQPV
                     VPLVIAVASLAVTTLIFAAGHPQPLTRAGEALLAALRERHAPDPGWQRSDPALLLCLA
                     LCGAAAVRGLVPGGVGHVLAPPASDDDAPVGLDAGLDPGGSGCDSGGDGGCGSGCSG"
     gene            21301..>21975
                     /locus_tag="PK144_02245"
     CDS             21301..>21975
                     /locus_tag="PK144_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64576.1"
                     /translation="MNPDPHRPGTAANDAGPPALSTPTVAVADADCAAALPPACRDPA
                     LAALYQRLLAYEFDDPQAALPFSRRLARDNGWTHRGALRAIDEYRRFVFLALAAGHPV
                     TPSDQVDQVWHLHLVYTRMYWLDFCPQVLGRSLHHGPTRGGAAEGGRYDEQYRRTLAS
                     YERCFGHPPPADLWPAPELRFGRDLGFVRVNRHACWLLPRSPEAWRFVLHRRFGRLLR
                     SLRRTLA"
BASE COUNT         3149 a         8105 c         7558 g         3163 t
ORIGIN      
        1 gacatcgcct gcggccggca ctgccagcgt cgaggtgctg cgcggcaacg gcctgcgcga
       61 ccggcgcgag ctgcaggtgc gcatccggca ggccgtgctc gcggcgcgcg aatcggtgct
      121 gatcaccacg ccctacccgc tgctgtcgcg ccgcctgctg cgcacgctgc gccggcgcgt
      181 cgccgatggc atcgaggtgc gcatgctgat cccggagcac tccgacgtgt atgcggtgga
      241 tctcgcccgc acccggctga tccgccagct cgtgcgccag ggcgtgcgcg tctaccgcta
      301 ccgccggccg atgcacacca agacgctggt cgtcgatgac gccttcgcgc tggtgggctc
      361 gttcaacctc gacatctggg gcagcgccca caacctggag atcgggctgg cgatccacga
      421 taccggggcc gctgcccggc tcgccgccga cttccgggcc gatctcacgg atgcgctttc
      481 cgatccgccg cccccgagcc cgctgccgcg gcgcgcgctc gaatggctgg cccagcgggc
      541 gctgcgcatc ggcctgctgc atgcgacgcg ccccggacgc gagggggccg gaaattgacc
      601 ctgtacggac cgcgacgtta acttagtctt cctttgcccc gcggtacccg acgatggcga
      661 cgaagaaagc gcccgcgacc gacatcgaaa gcgcgctgaa ggaactcgaa agcctggtcg
      721 aagcgctgga acagggagag ctgacgctcg aggacgccct cgcccgctac gagcgcggcg
      781 tgagcctcgc ccgcacctgc cagggcctgc tcggcgcggc cgagcagcgc atcgagttgc
      841 tgcagggcca ggccggcgcc gaatcgctcg tgccgtggac cccgccggcc gctgctgctc
      901 cgcaggactg atccgcacca tgcccgaagc ccgcatcgag gcctaccggg cgcgcgccga
      961 gcttgcgctc gatgcccgcc tgcctgccgc tgccacgctg cccgcccgcc tgcacgaggc
     1021 catgcgctat gccgtgctcg gtggcggcaa gcgcgtgcgt ccggtgctcg tctacgccgc
     1081 cggcgaggcg gtcggcgcgg cgcccgaggg cctcgacggc ccggccagcg cgctggagtt
     1141 catccacgcc tactcgctgg tccacgacga tctgccggcg atggacgacg atgacctgcg
     1201 ccgcggccgt ccgacctgtc accgcgcctt cgacgaggcc acggccatcc tcgccggcga
     1261 tgcgctgcag acgctggcct tccacgtgct cgcgcacgat ccgtcgatga cggtgccggc
     1321 cgatacgcgg ctgcgcatgg tcgagctgct cgcgcacgcc gccggctcgc gcggcatgtg
     1381 cggcggccag gccatcgaca tggctgcggt cgggcgcgag ctgacgctgg ccgaactcga
     1441 gaacatgcac atccacaaga ccggtgcgct gatccgcgcc gccgtgctgc tcggcgcgct
     1501 gtcgcagccg ggcgtggccg atgaggaact cgcgcggctc gaccgctatg cgaagtgcat
     1561 cggcctggcc ttccagattc atgacgacat cctcgacgtg gtcggcgaca ccgccacgct
     1621 cggcaagcgt cagggcgccg accaggcact cgacaaaccg acctatccgg ccctgctcgg
     1681 cctcgacggc gcccgcgccc acgcccgtgc gctgtacgac gacgcgatgc acgcgctcga
     1741 gcccttcggc gaacgcgccg cggtgctgcg tgcgctcgcg gcattcatcg tcgaacgctc
     1801 gcactgacgc gcgcccgcgg ccccataccg atcgcaccgg acgccttcat gactgccctg
     1861 acccccgtgt ttccactgct cgacaccatc gattccccgc aggacctgcg ccggctgccg
     1921 gagaacgtcc tcggccagct cgccaacgag ctgcgcgaat tcctgatcca gtcggtcgcc
     1981 cgcaccggcg gccacttttc ggccaacctc ggcaccgtcg agctgaccat cgcgctgcac
     2041 tacgtgtacg acacgcccga ggaccgcctg gtctgggatg tcggccacca gagctatccg
     2101 cacaagatcc tgaccgggcg gcgcgccctc atgggcacga tgcgccgccg ccacggtctc
     2161 gccggcttcc cgaagcgctg cgaaagcccg tatgacacct tcggcgtcgg gcattcgagc
     2221 acctcgatca gtgctgcgca gggcatggcg atcgccgccg aggccgccgg cagccgccgg
     2281 cgtgcggtcg cggtgatcgg cgacggcgcg atgaccgccg gcatggtgtt cgaggcgctg
     2341 aaccacgccg gccacctgaa gaacgacatc ctggtcgtgc tgaacgacaa cgagatgtcg
     2401 atctcgccga acgtcggcgc actgtcgagc tacctgaccc ggctgctgtc cggccacgtc
     2461 tattcgacgg tgcgcgaggg cggcaagcgc atcctcgccc acctgccctc aagcatgtgg
     2521 gagatggccc ggcgcgccga ggagcacgcc aagggcctgg tcacgcccgg cacgctgttc
     2581 gaggaactcg gcttcaacta cttcggcccg atcgacggcc acgacctgcc gacgctggtg
     2641 cgcacgctcg agaacctgcg cgaccagaag ggcccgcgcc tgctgcacgt gatgactcgc
     2701 aagggcaagg gctacaagct cgccgagaac gagccggtca cctaccacgg cgtcggcagc
     2761 ttcgatccgg cgagcggcat cccgccggcc aagactggcg gcggtccgac ctacacgcag
     2821 gtgttcggcg aatggctctg cgacatggcc gcccaggacc cgcggctggt cggcatcacg
     2881 ccggcgatgc gcgaaggctc cggcctcgtc gagttcgccg agcgctatcc gacgcgctac
     2941 ttcgacgtcg gcatcgccga gcagcacgcg gtgacgctgg ccgccggcat ggcctgcgac
     3001 ggcctcaagc ccgtggtcgc gatctattcg accttcctgc agcgcgccta cgaccagctg
     3061 attcacgacg tcgcgatcca gaacctgccg gtgctgttcg cgatcgaccg cgccgggctg
     3121 gtcggcccgg acggtccgac ccacgccggc tccttcgatc tctgctatct gcgctgcgtg
     3181 ccgaacctgc tgatcatggc accggccgac gaggacgaat gccggcagat gctgtacacg
     3241 ggcttcctgc acgacgggcc ggcctgcgtg cgctatccgc gcggcaaggg gccgggagtc
     3301 gcgccggtcg cgacgatgac ggcgctgccg atcggcaggg ccgaactccg ccgccagggc
     3361 cgggggaccg cgctgctcgc cttcggcgcg atgctgccgc tcgccgaggc cgtcggcgaa
     3421 cggctcgatg ccaccgtcgt caacatgcgc ttcgtcaagc cgctcgatac cgcgatggtg
     3481 ctggaactcg cgcgcacgca cgagcgtctg gtcacgatcg aggacggcgc cgtgatgggc
     3541 ggagccggca gcgcggttgc cgaatgtctg gccggacacg gcgtgcacac cgcgctgaag
     3601 ctgctcgggc tgccggatcg cttcctcgat cacggcgagc gcggtgagct gttcgccgaa
     3661 tgcgggctgg acatcgacgg cgtgctggcc gcgctggccg cgcagtccga tcactgagtc
     3721 cgacacccga accggcattg cggcaatgca atatcctgaa ttgctgcgac cacttgcgcc
     3781 gggctagtct gccgatcctg cccggcaacc ccggcaggcg ccgcgtgcgc cgcctgcgcg
     3841 cgcgccgacc gacattcacc ggacgcccga gcgcgtccgc tgcggagtcc gatcccgatg
     3901 agcatgccga ccgccgtgcc cgcaccgcag cagcagcagc cggctgcaat ccccgacatc
     3961 cagagcacgc cggacacccg ccgcctgccg atcgacaagg tcggcgtcaa ggatctgcgc
     4021 catccggtgc gcgtgcgcga ccgcagcgac ggcgagcagc acacggtcgc gaccttcaac
     4081 atgtacgtgc gcctgccgca tgacttcaag ggcacgcaca tgtcgcgctt cgtcgaggtg
     4141 ctgaaccgcc cggggcgcga gatcggcgtc gcctccttcc acgagatgct caccgagatg
     4201 aacgcccgcc tcgatgccgg caccggccac atcgagatga gctttccgta cttcgtcatg
     4261 aagacggcgc cggtctcggg cgtgcagagc ctgctcgact atcaggtgac gctgatcggc
     4321 gagcttgccg gcgagcaggc gaccacctgg ctgcgcgtgg tcgtgccggt caccagcctc
     4381 tgcccgtgct cgaaggaaat ctcgcgctac ggcgcgcaca accagcgctc gcacgtgacg
     4441 gtcagcgcgc ggctgtccgg gttcgtgtgg atcgaggaac tgatcgacat cgtcgagcag
     4501 gaagcctcgt gcgagctgta cggcctgctc aagcgcccgg acgagaagta cgtgaccgag
     4561 cgcgcctacg agaatccgaa gttcgtcgag gacatggtcc gcgacgtggc cgcacggctc
     4621 aacgacgatg atcgcatcgc cgcctacgtg atcgaatccg aaaacttcga atcgatccac
     4681 aaccactcgg cctacgcgct gatcgagcgc gacaagacgg ccggctgaac ctcggcccct
     4741 cccagaaacg ccatcggccg gtggatgcac cggccgatgg ggatacgcgc gctgtaaaga
     4801 ccgacccgga tcaggacgcc agccgctcca gcagcgcctg ggcacgcgag cgctggctgc
     4861 tgccgccttc ctgcaggact tcctccagca ggctcttggc accgagcgga tcgtccatgt
     4921 cgatgtaggc gagtgccaga tcgagcttgg tctcgacgaa ctcatcctcg ccccgggtat
     4981 cggccgtcga tgccgacacc gggggcgccg ccgccggggc aggaacgacc ggcaagggcg
     5041 ccggcgccgg cgcggacacc ggctggaacg agaagcccag atcgtcggcg agttccgccg
     5101 tagccggatc gaccgcggct ggcttcgctg ccgcttgcgc cggcgtgctc gtctggcgcg
     5161 gcggcaggaa ggcggcgaga tcgtcgccga agtcgagatc cgcgacttcc gccggtctgg
     5221 gcgccggcgc cgcagtcacc ggcttttccg gcatggccgg tggcgacccc aggtcggcat
     5281 cactccagcc gagatcggcg agcaggtcct ccggcgtggt ggccggcgcc ggaaccggca
     5341 ccggggccgg cttctcggcc ggacgcctga cttcgacggg ctcgaagtcc agcgacgggc
     5401 cgggcggcac gacggcggcc ggcggcagct tcgcaggagc gacggccggc gctttcggcg
     5461 ccatggtcgc cgccggtgcg gcctccggca tgtccatcgg cttgaagtcc agatcgctcc
     5521 agacctcgtc atccacgcgt gccggtgcgg cggccgccgc actggccggt gccggcatcg
     5581 gtgtgaccgg catcctcgga cttgtcgccg gcatgcccgg cggggtcgcc gcgaccgtgc
     5641 cgatgaacat cggatcgtcc gggcacagct cgcgtcccat gcgcgcaacc cgcagccaca
     5701 tcggatcggt gtcgccgtag acgagctggc gcagcggtcc gacctcgcgg cggaaggctg
     5761 cggcatcgcg cagggtgtgc agcacttcga gcaggcgcat gcgcagcgcg gtgttgtccg
     5821 gtgcggctgc cagcgccgat tcgagcagcg cccgggcttc ggcgtggttg ccgagcgaca
     5881 tcagcagcga actgcgctcc agcggatcag cgctggcagc cgcagcaccc gccgccgccg
     5941 gcaggggcgc tgcgcccggg gcggcatgat cctcggcgag cgtgatgctc tcgaacatct
     6001 cgtgctcggc gtcctgcgcc cggcgggcgc ggcgcatcgc cagcagtccg ccgccgagca
     6061 gcgcggccac ggccacgccc agaccggcaa gcaccagcgg gtcctcgaag gcgagcagga
     6121 cgctgtcgag gaaatccggc tccggagccg gcttcaccgg cggtcgggcc gccaccggcg
     6181 cgggcgcggg tgccggagcc ggtgtcggct gcgttggcgt cacggatgcc ggtctggggg
     6241 gctcggcaac cggtggcgtc accggcaccg gcatacgctc ggccgggggc agcgtgtcga
     6301 tgcggttctg cagatggtcg aggccggcgg cctgcagccg cagacggccg ttgtcgagtt
     6361 cgatctggcc ataaccgccg ccggcgaccg ggtccggtcc gcgcggctgg tcagtcatgc
     6421 ccatgcccgg tgcgggcagg gcattgccgc cgctctgacc ttcgggcggc agcagccgga
     6481 cctgtgcgcc ggccggcggc atctcgggcg gctttgccgt ggcgggcgcc gggcttgccg
     6541 gcggcctggt cgcgacagcc ggcggggtca cgggcagcgg ctgggccgga gccgggactg
     6601 ccggggccgg cggattgccc tgccagccgc tttcggccgg catgcgcagc atggcaccgg
     6661 cgcgcagccc gtccggcgag cgccgcgaga acgcctcggg attggcatcg tacagacggc
     6721 gcacggcctc gcggttcgac atgtcggccg ggcgcaggcg atcggcgatg ctccagaggg
     6781 tttcgcccgg gcggatcggc ccgtacgtgc cgccgccggc cgatggctgg cctgcatatc
     6841 caccgatcgg gctcgcggcg gttgcgcgcc gctcgttcgg gcgcaccgga tcgagcagga
     6901 tcgcgaactc gcgcatcagc ttgccgcgcg gactgccgag ttcgaccaga aagttcaggg
     6961 ccggctcgcg taccggacgc tgcgaggtga tgcggatgac gctgcgtccg cccggctggg
     7021 cctcgacggc gaactgcagg tcggacagcg ccccggtgcg atcgagcccg gcctgcgcga
     7081 aggcctgcgg cggcgcgaga cggacgctca ggttctgcag ttcgtcgggc gcgaccgaca
     7141 gcagctcgac ctgggccctg aggcgttgac ccagtccgga ctggacctgg atcggaccga
     7201 cgccgatggc ggcggcaaca cccggtgcca acagcagtcc ggcgagaagg gctcgactgc
     7261 gttttttccc tttattcatt gtcggtcggg tctcggcgtc cgtcgcgcgg gatcaaaaag
     7321 accgccggca cgaagggtcc ggcggtctgc ggatcccatt gattataaag gcgatcatga
     7381 atcgccccgt gcgtcattct gctgttccga tcagcgtttg ggcgatttgg acgccattgg
     7441 acgcgggcag ggccacatta tccgccacta cccacagatt caagccccgt tccccggcgg
     7501 cccgcagtcc gccgacccgc acggccggct gtccgagacc atcctcggcc ggtgaaaccg
     7561 gcgcttcatc ggcctccagc acttcgaggc cgggcacctc cagcagccgc tcccgggccg
     7621 cggcggggga cagcgcggcc gtgcagcgca gatgcaccac acaggccagg ccgtagaaca
     7681 gcggcacgcg cagcaacgtc gtcgcgacgc tgccgggcgc gagaccgagc acctgttcga
     7741 gtccggcatc gagccggcgc tcgccgccac cgcggccttg cgcatcgaga tcctgccatt
     7801 cgggcagcac gttgaatgcg agccggcgcg gatagatctg cggattcgag ggccgggcgt
     7861 tcagcagcgc gatggtctgc tgcgcgagtt cctcgaccgc ggcgcgaccg tcctccgatg
     7921 ccgccctgag cacggtcagg cccacgtgcc cgatgccggc cagggccgcg agcggcatca
     7981 gcacccgcgc gagcgcgagc gtcacggcat cgggcagcac cagcgcatcg gcacggcgcc
     8041 agcggttttc gtcgtggccg gcgatcgcga ccggggccgg agcctcgccg gcaagccagc
     8101 cgctggcgtc gagcacgcgc gcaccggcgc ggcgcgcgag cggcagctgt gccgcggcaa
     8161 gcgcggcatc gtcggtggcg atgacgagcg cgatgcgcga ccagtcgcag ccggcggccg
     8221 gctgcacgcg caggccggca tcgccgtagg ccaggcgctc gccttcgacg tcctcgccgc
     8281 ccagcgcgat gagttgcgcg gccggcagtc cgtcggcggc aaaggcggcg agcatcgctt
     8341 cgccgagcag gccatcggcg ccgagtacgg cgatgacggg ggcgggatgg tcatgggaca
     8401 tggtggatcg gttcctccgg ccgcaaaagc accgggcccg tcgcgtgcgc gacgggcccg
     8461 gtgaacggac agcttagtga tgaaaagccg gttcaggctt cgagcaggat gcgcagcatg
     8521 cgccgcagcg gctcggccgc gccccagagc agctggtcgc cgcagacgaa ggccgagacg
     8581 tactcggggc ccatgttcag cttgcgcacc cgcccgacgg cgatgtcgag cttgccggtg
     8641 accgccgtcg gcgtcagctc ctgcaccgtg agcgggcgct cgttcggcac gaacttgacc
     8701 cacgggttcg agccgcggat cagcgcctcg atctcggcga gcggcaggtc gcgcgtgagc
     8761 ttcagcgtga gcgccaggct gtggcagcgc atcgcgccga tgcgcacgca caggccgtcg
     8821 accgggatgg tctcgcgggt gccgaggatc ttgttgacct cggcctggcc cttccattct
     8881 tccttggact ggccgttgtc gagctgcttg tcgatccacg ggatcagcga gccggcgagc
     8941 ggcgcgccga agtgctgagt cgggaagtcg ctgctgcgca tcgtcgcggc gacctggcgg
     9001 tcgatgtcga ggatcgccga ggccggatcg gcgagcagtc cgctgaccgc gccgttgacc
     9061 acgcccatct gcgtcagcag ctcgcgcatg ttctgcgcac cggcgccgga ggcggcctgg
     9121 taggtcatcg agctgaccca ctcgaccagc ccggcctcga acagcccgcc gacgcccatc
     9181 agcaggatcg agttcgtgca gttgccgccg atgtagttgc gcacgccctg cgcgagcgcg
     9241 gcatcgatga ccttgcggtt gaccgggtcg aggatgatga cggcgtccgg ttccatgcgc
     9301 agcgccgagg ccgcgtcgat ccagtagccg tcccagcccg cggcgcgcag ctgcgggtag
     9361 atctcgttgg tgtagtcgcc gccctgacag gagatgatca cgtccatcgc cgtcagcgcc
     9421 tcgaccgagc gcgcatcggc gagcggcgcg atcggcttgc cgatgtcggg gccgctgccg
     9481 ccgacgttcg aggtcgtgaa gaacaccgga tcgatcagcg cgaagtcgtt ctccgcgcgc
     9541 atccgctcca tcagcacgga accgaccatg ccgcgccagc ccaccagacc cacacgtctc
     9601 attgccttgc ctctttcgtt cgggtgacgg cggaccacgc agccgccgca acacattgaa
     9661 tcatcaatcc tgtgccggcg cccgcctcca gcggaggcag gcaccggcgt tccgggcgtc
     9721 agtccgcgag cgcggccagc acggcttcgc ccatcgccac cgtgccgacc gtctcgcagc
     9781 cttcggcgcg gatgtcggcg gtgcgcaggc cctggtcgag cacgcgcgat acggcgcgct
     9841 cgacgcgctc ggccagatcg gcacggccga gcgagtagcg cagcatcatc gccaccgaca
     9901 ggatggtcgc gagcggattg gccacgccct tgccggcgat gtccggagcc gagccgtgga
     9961 tcggctcgta caggcccttg ccgtgcgcat cgagcgaggc cgacggcagc atgccgatgc
    10021 tgccggtcag catcgaggcc tcgtccgaga ggatgtcgcc gaacatgttg ccggtgacca
    10081 tcacgtcgaa ctgcttgggg ttgcgcacga gctgcatcgc ggcgttgtcg acgagcatgt
    10141 gcgtcagttg cacgtccggg tagtcctttg cgacctcctc ggccacgtcg cgccagagct
    10201 gcgtgcattc gagcacgttc atcttgtcga ccgagcatac ccggccgccg cgcttgcggg
    10261 cggcctcgaa ggcgacgcgc acgatgcggc ggatctcgga ttccgagtag atcatcgtgt
    10321 tccagccgac gcgctcgccg tcgcgcagct cgatgccgcg cggctcgccg aagtagatgt
    10381 cgccggtcag ctcgcgcacg atcaggatgt cgagcccggc caccagctcc ggcttcagcg
    10441 acgaggccgc cgcgagctgc ggatagacca cggccgggcg caggttcgcg aacaggttca
    10501 ggtccttgcg gatcgcgagc agcccgcgct ccgggcgctg cgggcgcggc agcgtgtcgt
    10561 actgcggacc accgacggcc ccgagcagca cggcatcggc ggcctgcgcc ttcgcgcgcg
    10621 ttgcgtccgg gtacggctcg ccggtggcat cgacggcgca gccgccgagc agcgcggtgt
    10681 cgagctcgac atcgaggccg tagccgctgc gcagcacttc gagcacccgg acggcctcgc
    10741 cgacgatctc cggaccgatg ccgtcaccgg cgaggacgag aatcttcttg gtcatgccct
    10801 gatcacctct gttgaatcgt tgctgcaacc gcccggggtt gctcatccgc tgacgatcga
    10861 ccgcagggcg cccgacttga acagcgcagc cacgagcagc ccgccggcca ccagcacgaa
    10921 ggccagccac cagccctgcc cgctgctgcg tgcctcgggc tgacgccggc gccgcgccgt
    10981 gcgcgccggc actgctgccg gtgcggatcc gccctgatgt gcccccggct gcggacgcga
    11041 cgcccggacg gtttccagac gcagcccgcc ggacatcgac ccggacctcg acgactccgc
    11101 tgcctgcgcc gccgctgcag ccccggctgt cttcggcatc gactccagat gcgcgagcac
    11161 gcgcgcggcc gcattcatga agtcgtccgg attgctgatc tcgaaatcgt gctggtgcac
    11221 ggccgagttg cgcagcgtcg cgatccagcg cgcggtcttg acgatgtgcg gcggcagctg
    11281 tctttcgacg ctgttcagct tctcatgcag gcccttgccg ttcgccccga gccgctcgac
    11341 cagcagggtt tcgatcgccc gggcgttgcg taccgccatc tcgatgctcg acatccctcg
    11401 cctcctgaat ccgccatcgg tcggccgcgc atcatgccgc gagcggctgc cggcattcga
    11461 gtccgtcgtc accgctgaca accggccgcc tctcccacgc cttgccctca gttccacagc
    11521 cgctgcacgc cgaagccgtc gaacagggtt tcattgctga ccagcacggc ctccacgcac
    11581 agggcctggg caatcagcat ccggtcgaac ggatcccggt gcgcacccgg cagcaatccg
    11641 gctcgcagtg catggaccgc cgcgatgtcg agccagtcga aggactgatc acgcacgtaa
    11701 gtgtcgatgt ccgccagcaa ctcacccacg ccctccagct ttccaagccg gtgcttcgtc
    11761 gcgatctccc agatcgaggc agcactgacc agcacggcat tctgccggtc ggcgatgaat
    11821 gagcggactt tctccggcag acgctcatcc ccggcgaacc accagagcag ggcatgtgta
    11881 tcgagcagca gacgcactag cgctcccagg cggcgaggac ttcttccggc aaagggtcga
    11941 agaacgcgtc atcgacccgg attctcccgc gcaaccgacc gaattgacgc tgaccagccg
    12001 cacgggttgt caccggtacg agcttcacgg cctgtgccgc ctcaccggtg atgatgatgt
    12061 cctcaccacg ccaggccctc tcgatgagaa ccgccagatg ctcacccgct tcatgaaccg
    12121 gacaggtttc catcgcagac ctccggacgc cggaattcag ccgataccaa cttgactcac
    12181 tgaaacagcc acggcgcttc ctgccgccgc ttcgcctcgt aggcgcggat gtcaccggcg
    12241 tgtctgagcg tcaggccgat gtcgtcccag ccgttgacca ggcattcctt gcgaaacgca
    12301 tcgatgtcga agtcgatgac ctcgccatcg ggcgtggcga tcgtctggcc cggcaggttc
    12361 acgaacagct ggtagcccgg cgtcgcttcg accgcccgga acagccggtc gacggtctcg
    12421 gccggcaggc ggatcgccag cacgccgttc ttgccgcagt tgctgaagaa gatgtcggcg
    12481 aagctcggcg cgatcacgca gcggatgccg tagtcctcca gcgcccagac cgcgtgctcg
    12541 cgcgagctgc cgcagccgaa gttctcgcgc gcgagcagca cgctcgcgcc cgcgtagcgc
    12601 ggctggttca ggctgaagtc cggattgcgc ggccggccgg cgcagtcctg acccggttcg
    12661 ccgacatcga ggtagcgcca ttcgtcgaac aggttcgggc cgaagcccga gcgcttgatc
    12721 gacttcagga actgcttcgg gatgatcgcg tcggtgtcga cgttcggccg gtcgagcggg
    12781 acgacgaggc cgtggagttc ggtgaacggc tgcatggcca tctcctcaga aatcttcgct
    12841 cagaagctgc gcacgtcgac gaaatgaccg gcgatcgcgg ccgccgcggc catcgccggg
    12901 ctgaccagat gcgtgcgtcc gccggcgccc tggcggccct cgaagttgcg gttcgaggtg
    12961 ctcgcgcagt gctcgccggg gccgacgcga tcggcgttca tcgccagaca catcgagcag
    13021 cccggctcgc gccattcgaa gccggcatcg aggaacagct tgtcgagccc ctcggcctcg
    13081 gcctgctgct tcacgaggcc cgaacccggc acgaccagcg cctgcttgac gctcgcggcg
    13141 accttgcggc cccggatgac ggcggcggcg gcgcgcaggt cctcgatgcg cgagttcgtg
    13201 cacgagccga tgaacacgcg atcaagccgg atgtcggtga ccggctggcg ggcggccagt
    13261 cccatgtagg cgagcgcctt ctccatgccg gacttcttga cggtgtcggt ttcggcggcc
    13321 ggatcgggca cgttctcacc gaccgcgacc accatctccg gcgaggtgcc ccaggtcacc
    13381 tgcggcgcga tggcggcggc gtcgagttcg acgacggcat cgaagcgcgc atcggcatcg
    13441 gagtgcagcg tgttccacca ggcgacggcc ttgtcccagt ccgcgccctt cggcgcgtac
    13501 gggcggccct gcacgtagcc gagcgtcgtc gcatcgaccg cgaccatgcc ggcgcgggcc
    13561 ccggcctcga tcgccatgtt gcagaccgtc atgcggcctt ccatcgacag cgcccggatc
    13621 gcgctgccgc cgaattcgat cgcgtaaccg gtgccgccgg cggtgccgat gcggccgatg
    13681 atcgcgagca cgatgtcctt ggcggtgacg ccggggccgg cctcgccgtc gacgcgcacc
    13741 agcattgcct tcgccttttt ctggatcagg cactgcgtgg ccatcacgtg ctcgacctcg
    13801 gaggtgccga tgccgaaggc cagcgcgccg aaggcgccgt gcgtcgaggt gtgcgagtcg
    13861 ccgcagacga cggtcatgcc cggcagcgtc gcgccctgct cggggccgat cacgtgcacg
    13921 atgccctgac gcacgtcgtt gatcgggaag tacgcgaggc cgagttcgcg ggcgttggcg
    13981 tcgagcgtct cgacctgcgt gcgcgagacc ggatcctcga tgtcgccgat gccgccggcg
    14041 cgcggcgtgg tcggcacgtt gtgatcgggc acggccagca tcgacgcctt gcgccagggc
    14101 gcgcggccgg cgagcttcag gccctcgaag gcctgcggcg aggtcacctc gtgcacgagg
    14161 tggcgatcga tgtacagcag cgcggtgccg tcggcgtcgg tgcgcacgac gtgcgcagcc
    14221 cagatcttgt cgtagagcgt ttggccggac atttcagcgt cctcgttcgg gcagtgcgcg
    14281 gactgtacgc cgggcctgaa catgacacca attcatgttt ttcatactcg gcatgacgcc
    14341 atggaatact tgcacgcccc cctcccgccc ggtgccgcgc atggacatcg ccagcctgca
    14401 ggccttcgtc gccgtcgccg acagcggctc gttctcggtc gcggccgaac ggctgttttt
    14461 aacgcaaccg gcggtcagca agcgcatctc ggcgctcgaa gccgaactcg gcgtgcggct
    14521 gttcgaccgc ctcggccggc gcgtgctggt caccgaagcc ggcgggacgc tgctgccgcg
    14581 ggcgcggcgc atccttgccg agctcgacga cagccgccgc gcgctcgccg acctgtccgg
    14641 gcaggtcgcc gggcgcctga gcgtcgccac cagccaccac gtcggtctgc accggctgcc
    14701 gccggtgctg cgcgcctaca cggcgagcta tccgcaggtg cagctcgacc tgcgcttcat
    14761 ggcctccgaa gccgcctgcc tggcggtcgc cggcggtgat gtggagctgg cgatcgtcac
    14821 gctgccgctg gccgccgaag ccccgctggc gctgcagacg atctggaccg accggctggt
    14881 gctggtggcg caccccggcc atccgctcgc ccgcctcaag cgcgtgtcgc cgttccggct
    14941 ggtcgagcac gcggcgatcc tgcccgagca cggcaccttc acgcgcgagc tgatcgagcg
    15001 ctgcttcgaa ccgctcggca tccggttgcg cgtggcgttt acgacgaact tcctcgaaac
    15061 catcaagatg atggtgtcga tcggactcgg ctggtcgctg ctgccgcgca cgatgctcga
    15121 tgcgcagctc gccgtgctcg aactcgaagg catcgcaccg acccgcgcgc tcggcctcgt
    15181 cgcgcacgcc ggccataccc cgtcgaacgc cgcgcgcgcg ctgctcgggc tgctcgcgcc
    15241 cggcgaaggc ggcgggccgg ccgaacccga accgtggcgc ggctactgaa cgggcagcgg
    15301 ttcacgctgt tatgctttcc ggcttgccgt tcggcacccg ccgtaccgga tcccgtagct
    15361 gccgcctgcc gcccatgaac aagaccgacc gcgaacgcac catcgccctt gccgccatct
    15421 tccaggcggc cgcgctggtg cgcgatgtcg cctacgccgg ccaggccgat gccgccgaca
    15481 gcgaagcctg cctgcgcagc atcttcgcga tcgactcgcc cggcatcgcc gaggtctacg
    15541 gcggcctgcc cggcctgcgc cgcggcctgc ggctgctggc ttcggagctg cgcacgccgc
    15601 gcgagatgga aatcgcccgt tacaccgtca atctgctcgt gctggagcgc aagctgcgcg
    15661 ccagcccggc gatgaccgag cgcctgcgcc agggcatccg cgacaccgcc cgtcgcctcg
    15721 atcattttcc ggtcacgcac gagaacatcg tcgccgccct cggcgagctg tacgccgaca
    15781 cgatctcgac gctgacgccg cggatcatgg tcaacggcga gcagatgcac ctgtcccggc
    15841 gcgacaacgc cgcgcgcatc cgcgcgctgc tgctcgccgg catccgggcc gcctggctat
    15901 gggcgcaggc cggcggcggc cggctgacgc tgctgctgcg ccgccgggcg ctgctcgccg
    15961 aatcccagcg cctgctgcgc gaactcgggg attgaatccc ggcggtggtc cgaccaccgg
    16021 acgaatccga atattcgcag gcaggttccg gtcttgctcc ctgccgcaac ggcccccgcg
    16081 ctggctaagg tccgggtctg atcagtccgc ggacgagact gcaagcccgg gacggccctg
    16141 cgcatcatca ccggccggcc ccccggatac atgtgcgcgc gagtcatgcc aggaaaggcg
    16201 ggcatcgccg ccacccgacg ccaatcccgg acgtaccgcc cacccggtcg caggaaggcg
    16261 cccggagttc attcatggat caaccgtccc tgccagccgc cggcgctggc catctgccgc
    16321 cggacccgct gaacgcctcg ctgccggaaa ccgccggcat gccagcggtc gagaccgcca
    16381 tcctgaactg gctgtggcgg cgcgagcacc gcatcgccga taccccggcg cggctgatgg
    16441 tcgcgctgcg cgatggcgcg ctgcccgagc tgtgcgccgc ctcgctgcgc cggcacttcg
    16501 ggcgcctcga tgccgctgcc gcgccgccgc ccgaactcag gctgcacgat ccggtcagcc
    16561 cgcagctgcc gcaggccgac atcctgatcg gcccccagca tgcgctgatc gacgccctgc
    16621 ccgccgccga ggcgcgccag gcccagtggc tgctcgatgc cccaccggtg ccggtcgcgc
    16681 gccgcgcctg ccagcggctg gccccggcct tcgcggccgg gcgcgtgcgc gtgctgccgg
    16741 ccacgggctg cgaggccgat gccgtgctgc cgccgctgcc gctgcacgcc gccaccgcac
    16801 tgctcgatgc gcaccgcccc ggcacgacca cgctcggcgt gccggccact ctcgcccagg
    16861 tgcgctcgat cctgcgcgag ctgcagcagc tcgcgcccgg cacggcgctg gtcgccgtgc
    16921 atccgtgctt tcgcagcgcc catcgcgccg ccctcgccgc ctcgctgctg aacgcaccgg
    16981 ccggcggccg catcctggtc gcgaccccgg cggtggcggc gcagctgcag gccgaccggc
    17041 ggctggtcgc gctcgatggc ggtgaagccg ccaccggcgg ccacgaggcc ccggtgttga
    17101 acgtgctctg gcggctgcgt ggcagcgagg cagcgccgca gtccgccgaa ctctgcccgg
    17161 cgccgccggt ggcggtgcgc aagctgcgcc gcgagcgctg gctgtgggac cgtgccgccg
    17221 gccgctggca gcactgccag ccgtcgacgg cgctgccggc cggcagcatc gtgcgcctga
    17281 acgccgagga aggcggctat gacgccacgc tcggctggat gccgcacgcg cggctcacgg
    17341 tcgtgccggc cctcaccgtc gactgaaggc ctggcgtttc gcagacgccg ccttcagccg
    17401 cgccgccagt cgtggtagcg ccgcacccag ccgagcaggc gctgcggcgc gtgcgcgcgc
    17461 ttccaggtgc cggccacggc cttgccggac tcggcccagg tcggatagac gtggatggtc
    17521 gcgagcagct tttcgagccc gagcccgtgg cgcatcgcca gcacgaagcc ggcgatcgtc
    17581 tcgccggcgt gcgcgctgac gatggtcgcg ccgaggatgc ggtcgcgccc cggcacggtc
    17641 agcacgcgca cccagccctg cggctcgctc tcggccagcg cccggtcgga atcatcgagc
    17701 cggtagacgc tgctctcgaa ggcgatgcca cgctcgcgcg cctcctgctc gttgaggccg
    17761 acgcgcgcga tctcgggctc ggtgaaggtc gcccacggga tcaccgagta atcggcccgg
    17821 aaacgcctga actgcccgaa caggccgttg accgccgcat accaggcctg gtgcgcggcc
    17881 gtgtgcgtga actggtacgg cccggcgaca tcgccgcaga ccagcacgtt cgggaagcgc
    17941 gtctgcagga aggcatcggt ctcgatcgtg cgcgacttcg tgagcccgat gccgagggct
    18001 tcgagtccga ggccgtcggt gaccggcgcg cgcccgaggg cgaccaggat cgcgtcgaaa
    18061 ccgagccgca cttcggtgcc ggcgtgttcg gccaccagca cccggccgcc cccggcgtcg
    18121 cgctcgcagc gcagcgcccg gtggccgcag cgcacgtcga tgccctcggc ggcgaatacc
    18181 gcgccgatgt gcgcggacac gtccgcgtcc tcgcgcgcca gcagccgcgg cagcatctcg
    18241 acctgcgtga cgcgggcgcc gaggcgcgcg aaggcctgcg caagctcgca gccgatcggc
    18301 ccgccgccga gcacgagcag gcgctgcggg cattcccgca gcgcccagag cgtgtccgag
    18361 ctcagatagc cgccctcttc gagcccggga atcggcggca cgaacggccg ggcaccggtg
    18421 gcgatgacga tggcgcgggt gtcgagccgg cgctcgctgc catcggccgc cgtgatcgcg
    18481 accgtccacg gcgacagcag ccgcgccgtg cccgccagca cctcgacgcc gagaccggtg
    18541 tagcgctcga ccgaatcgtg cggtgcgacc gccgcgatca cgcgctgcac gcgcgccatg
    18601 acctcggcga agtcgaagcg ggcgctggcc tcgccgatac cgtaatccgg cgcacgccgc
    18661 tgctcggcca ggaagcgcgc cgtgcgcagc agcgccttcg acggcacgca gccgcggttc
    18721 aggcagtcgc cgcccatctc ggcgcgctcg accagcgtca ccttcgcctg caccgccgcc
    18781 gcgatgtagg cactgaccag cccggcggaa ccggcaccga tcacgatcag gttgcgatcg
    18841 aagcgcttcg gcttcggcca cggccggtag acctgatgcg cctgccaggc atcgagcgcg
    18901 cggcgcgcaa gcagcgggaa cacgccgagc agcacgaagg acgcgatcag ccccggcgac
    18961 agcaggccgg agagcgactc gacccgcgcg agctgcgtgc cggcgttcac gtagaccacc
    19021 gtgcccggca gcatgccgat caggctgacc cagacgtagg tgcgtaccgg cagcgccgtc
    19081 agcccgctgg cgaggttgat caggaagaac ggcacgaccg gcaccagacg cagcgtgaac
    19141 aggtagaagg ccccgtcccg ctcgatgccg gcatcgagcg cgcggatgcg ctcgccgaaa
    19201 cgcgcctgga tggcgtcgcg cagcaggaag cgcgcgacca gcatcgccag cgtcgccccg
    19261 gtcgtcgccg cgatcgcaac cagcagcgtg ccggtgccga gcccgaacag cgccccggcg
    19321 gccagcgtca gcaccgttgc gcccggcacc gacagcgcgg tgaccgcgac gtagaggccg
    19381 gcgaagccca gcgccgccgt cagcggctgc gcgtcgcgcc aggcgagcag ttcgccgtgg
    19441 cgcgccttga gcgccgcaag gctcagccac tcccccgcac ctgacgcgaa gaaccccgcg
    19501 atcagggcga tgaagccaaa ggcgaacaac agcttgcggc gattcatgcg ggaggcctcc
    19561 ggcgacgggc gggcggtgga caggcagcag cgcacgcttt ccggccgccg ccggcaccgg
    19621 atcgggcctc gcccgcttgc gggggcggcc ctggagtggc gcaggtgcgc aggccatacg
    19681 caggcagcag cggtgcggat gcaggcgctg cagcgcagca gggccgccgc gcagcatagg
    19741 cgccgcaccg ctgcggtgcc ggcgtgccag ccaccgccac cggccccgac accggccccg
    19801 acaccggagc gcaacctgcg gcgcgtagct ttcgcgtcac gggcgccggg ccggcaccgg
    19861 cgcccgcccc gcccgcccgc atgcacgcca atcaggagcc cgaccatgga cttccgcagc
    19921 cgttacgccg ctttcctcga cgagctggat gccatcgccg aaacccatga cgaactgttc
    19981 gacaccgagg tgcgcgagcg cctgcgcgag gtcatcgact accacttcgt ctgggatcag
    20041 cccatcggcg aggacttccc gcgggtcttc gcgatgttca gcgacacggc cgatgccctg
    20101 gtcgccgcgg ccgtgcgcag cttcatcgag gaagcctgcg ccctcgcccg cgccgaaggc
    20161 atcagcaccg ctgccgcccg ccatgcggcc atcgaggacg acacgctgct cggcgacgaa
    20221 gggggtttcg gggattatct ggtcgacacg gaactcacgg acgctccggt gccgccggcg
    20281 tccgacgcgc tatacctgtc ggatgcccgg ccctgatcgt ccttcccccg ccgcttcgcg
    20341 cggccgctcc gcacgccggc ccggccgcgc actgctgctg tgcttcgcgc tcgccctcgg
    20401 cggctgttcc tcgctggcct ggctgcgcct gccgtggtcg ctgacgcacg ccggccacgg
    20461 cctgctggcg tggttcgtgc cgctggccgt gctgtcggtg ctgcttgcgc gcggcctgca
    20521 gcagtggctg cgcggaccga aggtctccgc tgccggaccg cagccgctgc tgacgacgta
    20581 tgaggcagcc ttcctcgccg gcggcgaggc gcgggtcgtc gaggtcgcct gcgcgagcct
    20641 cgcgcagcgc ggctggctga tcacgaccca ggactggcag gcgctgaaac cgacgaaaat
    20701 ctgcggcacg ccggaagacc cgctcgaacg cgccgtgctc gccgccatcg cccgcgacgg
    20761 ccgcccggcg cacctgcggc gcgtcgcggc actgcgcatc gctccggccc tgtatcgacg
    20821 cctgcacgcg ctcggcctgc tgcacgacgc ccggacactg cagcgcctgc gccgctggcc
    20881 ggcgctgctg accggcaccg tgctgctggc cggcctcgcc ggcatcggct tcgggctgct
    20941 gcaccagcag ccggtcgtgc cgctcgtgat cgccgtcgcc tcgctggcgg tgacgacgct
    21001 gatcttcgcc gccggccatc cgcagccgct gacccgcgcc ggcgaggcgc tgctcgccgc
    21061 gctgcgcgaa cgccatgcgc ccgaccccgg atggcagcgc agcgatccgg cgctgctgct
    21121 ctgtctcgcg ctctgcgggg ctgcggccgt gcgcggtctc gtgcccggcg gcgtcggtca
    21181 cgtgctggcc ccgcctgcca gcgacgacga tgcaccggtc gggctcgatg ccgggctgga
    21241 tccgggcggc agcggctgcg acagcggggg tgacggtggc tgcggcagcg ggtgcagcgg
    21301 atgaaccccg acccgcaccg gcccggcacc gcggccaacg atgccggacc gccggccctg
    21361 agcacaccca cggtcgcagt cgcggacgcg gactgcgccg cggccctgcc gccggcctgc
    21421 cgcgacccgg ccctcgcggc gctgtaccag cggctgctcg cctacgaatt cgacgatccg
    21481 caggcggcgc tgccgttcag ccggcgtctg gcgcgcgaca acggctggac gcatcgcggc
    21541 gcgctgcggg cgatcgacga ataccggcgc ttcgttttcc tcgcgctcgc tgccggccat
    21601 ccggtgacgc cgtccgacca ggtcgatcag gtctggcacc tgcacctcgt ctacacgcgc
    21661 atgtactggc tggacttctg cccgcaggtc ctcggccgat ccctgcacca cggtccgacc
    21721 cgcggcggtg cggccgaggg cgggcgctac gacgagcagt accggcgcac gctggcaagc
    21781 tacgaacgct gcttcggcca cccgccgccg gcggatctct ggccggcacc cgaactgcgc
    21841 ttcgggcgcg atctcggctt cgtgcgcgtc aaccgccatg cctgctggct gctgccgcgc
    21901 agccccgagg cctggcgctt cgtgctgcac cggcggttcg ggcggctgtt gcgcagcctg
    21961 cgccggacgc tggcc
//
LOCUS       DAOQIE010000018        21766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1077_length_21766_cov_7.511031, whole genome shotgun sequence.
ACCESSION   DAOQIE010000018 DAOQIE010000000
VERSION     DAOQIE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21766
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1077_length_21766_cov_7.511031"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..492
                     /locus_tag="PK144_02250"
                     /pseudo
     CDS             196..492
                     /locus_tag="PK144_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            848..1381
                     /locus_tag="PK144_02255"
     CDS             848..1381
                     /locus_tag="PK144_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64577.1"
                     /translation="MTETEFLNLVHQGAGRGSFEEGWESGGAWEIHAQVVIAAFLRSG
                     YGITDARELAYPGSQEHCDFGFTHKGRKYAVELKVENKKDGKFGGMSLDQAMLTDVNK
                     LHAFNADELWFLVIARSDNAKQRLLELAMRGDSWIVDQEAGFLAALCNIRTQPHGLPW
                     QRYEKQALKLEQSPFYQ"
     gene            1647..2570
                     /gene="rarD"
                     /locus_tag="PK144_02260"
     CDS             1647..2570
                     /gene="rarD"
                     /locus_tag="PK144_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNF64578.1"
                     /translation="MSASSETVASPAPGLHPEFATGALYAVLAYLTWGLMPAYWKLVP
                     ELSAPELIAHRVVWSVFVTGALVLAFRRGRELRATLTDTRRLGVLLVTALLVSVNWLV
                     FVWAVLNGHVLESSLGYFINPLFNVLLGVLLLKERLRPLQGLAVALAAAGVLNQGWQL
                     QALPWISLALAASFGAYGLIRKLAPIEPLVGLTVETLLLAPLALAGLLWLDAQGLGHF
                     GHGTPARDLIIIGAGLITALPLLWFANAAKRLRFSTLGFFQYLAPSVQLMLAVLAYGE
                     AFTAVHAVTFGCIWAALALYTWDGRRALRIA"
     gene            complement(2605..3330)
                     /locus_tag="PK144_02265"
     CDS             complement(2605..3330)
                     /locus_tag="PK144_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64579.1"
                     /translation="MNPVITANEVELEFDDDRTARDETRVRSVRSRVRTRKRNHRWSL
                     RSRALFAALVASLMLTVFISLVLSLRMLSLSEENDELRHQNELRDAELTQVRPELERL
                     RADVTELIAKRLPGLRQLVLDQVVPIDDYYLRNVMFTLVKRGKRAGYEYHLTLSNDTL
                     QMVLPRVNIYLFDRHGVHIGSARIGGTGEHVEEVPDLGPGETVTKVGLVTIGEGEEEP
                     VFFRVRVAEPYTAEERESPNPQR"
     gene            3672..4340
                     /locus_tag="PK144_02270"
     CDS             3672..4340
                     /locus_tag="PK144_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008739123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF64580.1"
                     /translation="MARSAPAACGARTVDAILDATLALLDRDGERRTTTNRIAAAAGI
                     SPGNLYYHFRHREAIVLALFDRLVQALAARRAGIDRHPAAAAFAADFGALVQTVQARP
                     GLFRALPELLGQDAELTGRSHDWAAAEQGRLIRHFAALAAAGLGTPPPALQAPLAAAV
                     WHLLLARAWRGAEASEPLPSAILLAPLAAWLDAPTLARLGAGIGDAAYQSPQSSSSSP
                     SDSS"
     gene            complement(4292..4822)
                     /locus_tag="PK144_02275"
     CDS             complement(4292..4822)
                     /locus_tag="PK144_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 family protein"
                     /protein_id="HNF64581.1"
                     /translation="MSLRDQLLKAGLVSADAVQKAEKDRKQQKHQQQKQQSPSQRDAQ
                     KLEAERQRQAEAEAKRQRDRELNAKREAEKQRQANLARVRQLVEAHRLNDARAEDRYN
                     FPDGRFVRSVRVTAAQRRQIALGQIGIVRMDAKNFDFALVPRAEALKVQEIVPERLAL
                     LYEESDGLEEDDWGDW"
     gene            complement(4880..5698)
                     /locus_tag="PK144_02280"
     CDS             complement(4880..5698)
                     /locus_tag="PK144_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF64582.1"
                     /translation="MFLGSAAAPLELEVISRLPAQAGDKPPLLFVHGAFAGAWCWEEH
                     FLDFFAGLGYPSYALSLRGHAGSAGRDRLHWNRLADYVTDVAEVAVGLGERTILIGHS
                     MGGMVVQKYLERTPAPAAVLMCSVPPSGLMWSSLQLMWRDPMLWMEMGVITHAGPQYG
                     SRSLARRALFSPDAPDAVVDRLMNRAQEESSRVLADLTWADLPAVTLVRRTPLLVLGA
                     EHDALVPPSEVLETARRLGAECEIVPRVAHVMMFEPGWDRVARRVAGWLARQGH"
     gene            complement(5903..7138)
                     /gene="serA"
                     /locus_tag="PK144_02285"
     CDS             complement(5903..7138)
                     /gene="serA"
                     /locus_tag="PK144_02285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNF64583.1"
                     /translation="MGKVSLDRREIRILLLEGIAPSAVETFRAAGYTNLEQHRKALPE
                     PELIERLREAYIVGIRSRTQLTPAVLAEAKKLIAVGCFCIGTNQVALEAAASAGVPVF
                     NAPFSNTRSVAELVMAEIVLLMRGIPAKNALCHRGGWSKSAAGSREVRGKRLGIVGYG
                     HIGTQVGILAEAFGMQVHYYDIEAKLALGGAQPVHSLAELLARSDVLTLHVPETPQTR
                     NMIGAAELARLPAGAQLINASRGTVVDIDALAAALASGHVAGAAIDVFPAEPQGNDDE
                     FVSPLRAFDQVLLTPHIGGSTEEAQENIGFEVAGKLVRYSDNGSTLSAVNFPEVSLPA
                     HPGKRRLLHIHENRPGLLSAINDALSRQRINIAGQYLQTRGGIGYVVIDIDDDEHAGK
                     LALKELRAIDGTLRTRILY"
     gene            7283..8677
                     /locus_tag="PK144_02290"
     CDS             7283..8677
                     /locus_tag="PK144_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF64584.1"
                     /translation="MSDAAILARLGALLPPARLRTDADTLASHGRDWTRLYQPDPLAV
                     AFPESVAEVQALVAIANDTACPLVPSGGRTGLSGGAVAKSRELVLSFERMNRILGFDA
                     VDRSVTAEPGVILETLQDYAREHGLQYPVDFAARGSAQLGGTIATNAGGIKVIRWGMT
                     RDWVSGLKVVTGRGELLDLNRGLVKNNTGYDLRQLFIGSEGTLGLIVEATVRLAEAPQ
                     APAVMVLGLPDLEAIMSAFERLRAALRLTAFECFSAQALSYVTAHGQPRPFETETPWY
                     VLAEYDAAEERDTEAAMAAFETLAEDGIVLDGVLSQSDSQWRALWRLREDITESIAHR
                     LPYKNDVSVRVARVPEFLRAMDELFAREYPDFEVIWFGHVGDGNLHISVLQPPEMARA
                     DFVAACEQVSEHLFATLAALGGSISAEHGVGLVKKPYLSYSRDPAEISYLRAIRAAFD
                     PNGILNPGKLFDPA"
     gene            8868..10544
                     /locus_tag="PK144_02295"
     CDS             8868..10544
                     /locus_tag="PK144_02295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF64585.1"
                     /translation="MMTPDALPRSPLCRYLARALLLLLVPLVLLVFQDYGVSNDELVQ
                     STYGKLLLDYYRSGFSNDAALHYLDLYRYGGAFDMIAALLVPLSPFGEYETRHLLGGL
                     LGLVGLLGAWRLGRLLGGDRAGLLALVLLLLTPCFIGHSFTNPKDAPFATAMLWAVYA
                     IVRVQHELPRPSAHTLTLLGVTIGLALSVRVGAILLGPYLALGVLLWLAGRWRAGERP
                     ARLAADAARVALRCAPAFVLAYVVMGVFWPYAWQSPLNPLKTLGEFSHVAFPIETLAA
                     GEWYPVNAIPRWYVPAYLVFTLPEIVLAGLASGLAFALLRLRHRWPMSARAQSLYVLG
                     TAGVLPVLIAVALHTTAYNGLRHFLFVVPPLVVCAALALERMHEMLAARAPLHGRLHT
                     LALAALLAVQAGILGYLHPHQYIYYNVLIGGVPGAADRWELDYWGNSLPEAIDGLAEY
                     LTNEYEGAPIARRYRVAVCGKEESARYYFPDFLVFEPRWESADFLIALTNDGCDEQLR
                     LPNAVEIDRFGVPLSVVKDLRGYVLEYNPAFKPHRSRPKFLLYLRRYGKP"
     gene            10566..12359
                     /locus_tag="PK144_02300"
     CDS             10566..12359
                     /locus_tag="PK144_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-1,3-glucanase"
                     /protein_id="HNF64586.1"
                     /translation="MYDASVFRRPAFVAVALATLTAALVWWYAARPVDIGIDWHEGTW
                     NSASFAPFRAGQSPLTRNWPTPEQVENDLAHVKTAFRGVRTYAAIGAAQDVPALAARH
                     GLKVIHGAWLSSTPADNEAEVSALIEAANRYPQSVERVIVGNEVLLRRDLDAGQIIRH
                     LDRVRQSIRQPVSYADVWAFWLKNPQLADHVDYLTIHILPYWEDEPASVTDMEQRFVE
                     VIGKIKAAFPGKPILIGEAGWPTEGRSRGPAIADLPNAAHFLRRLPQLAAQYGFDYNV
                     VEAYDQLWKAQQEGTVGARWGLYDVDRQQKFGTTGPVDPFPEAGLRAGASVLGGLALA
                     LWLIPAGFGLTRALAVAGLAQLLAAGAIDTLWHAQRLAISPASVSWCLQRLLFFAADT
                     GLLDKPTVSELYRGYMDTTPALAEAWAWVRILATVVATGVLAALLRARLFGREAPGLA
                     RAARLGYLSYVAGALMISAMLAFNGRYLDIPIPEFWLPALGLPALWLAARLAGSPRGY
                     GLAALTAPGPARLKLGLWLLAGAVAVILGEGFAIVGEDFTAMHPDWSERAPLIWHAFF
                     WNRQILACALMLVWLALPLLAEWRRPVAAAT"
     gene            12370..12780
                     /locus_tag="PK144_02305"
     CDS             12370..12780
                     /locus_tag="PK144_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNF64587.1"
                     /translation="MHQTARQLLRFGAVGIAGLVIDVAVLEAALALGAGFYGGRALSF
                     LAAASGTWLLNRLWTFRSETARANRGLAGEWARYVALMLAGGSVNYAVYALCIETSAQ
                     ARTWPALAVAAGSLAGMVVNYASARWLVFRSGTA"
     gene            complement(12804..13697)
                     /locus_tag="PK144_02310"
     CDS             complement(12804..13697)
                     /locus_tag="PK144_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64588.1"
                     /translation="MTSAPLIEVRELVKQYPGLRAVDGVSFSILPGRCFGLLGPNGAG
                     KTTTIEMMEGISTPSAGRILYKGEPLGLRFREEAGIMFQSTALQDFLTVRECLRFFSQ
                     LYPRRVPAAELIRACALEDFLDRDHRKLSGGQRQRLLLAVALVNDPDIVFLDEPTTGL
                     DPQARRNFWQLVEDIKRRGKTVVLTTHYMEEAYQLCDSLAIMDRGRIVAEGSPDALLA
                     QHFDDRILQLPAEDLEGRALPPAYSVQRRRESAEISVRNVSTALSDLLAAGVPLERLS
                     IRPRTLEDLFLELTGKELREA"
     gene            complement(13747..14598)
                     /gene="hisN"
                     /locus_tag="PK144_02315"
     CDS             complement(13747..14598)
                     /gene="hisN"
                     /locus_tag="PK144_02315"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNF64589.1"
                     /translation="MSPTAPALPRHAPAELAPKQLGELIALAHLLADASGEVIRRHFR
                     APLAVADKSGAEGTMYDPVTVADRDAEAAIRALLAERRPQDGRYGEEHGFEPGDSGLT
                     WVIDPIDGTRAFITGMPLWGTLIALYDGERPVLGVMDQPWTGERFIGSRFGAELRDRD
                     GRRPLRTRACARLADAALYSTHPDLFERGHEKPAFDAIAARVRMTRFSGDCYAYCMLA
                     AGCLDLVVEAGLKPYDVQALIPIVEAAGGLMTGWRGENAAHGGRILVAGDARVHAEAL
                     AILSQVP"
     gene            14741..16690
                     /locus_tag="PK144_02320"
     CDS             14741..16690
                     /locus_tag="PK144_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF64590.1"
                     /translation="MPLLTFDRVSVAFGLAALLDNADFQIDAGERVCLIGRNGAGKST
                     LLKLVDGTLAPDGGELWRQPGLKIARLEQEMPETGDATVFEIVADGVPEVGRLLGEYH
                     AAAHALAHDHDAAHADALLRRMERLQHELEARDGWSLNQRVEQILTRLALPADVTLAE
                     LSGGWRRRVLLGRALVCEPDLLLLDEPTNHLDVEAIQWLEEHLLGFRGGVLFVTHDRA
                     LLTRLATRIVELDRGRLSSWPGDYPNFLERKAAALEEEARHNALFDKKLAQEEAWIRQ
                     GIKARRTRNEGRVRALKALREERSERRELQGRVSFNLEEAAASGKLVVEAKDVSYAWD
                     GAPVVRDFSVRVLRGDRIGLIGPNGAGKTTLLNLLLGRLAPDSGSIRLGTKLQVAYFD
                     QLRAVLDPESSVIDNLAEGREFIEINGERRHVISYLQDFLFSPERTRTPVKSLSGGER
                     NRLLLARLFSQPANLLVMDEPTNDLDIETLELLEELLLDYQGTLLLVSHDRAFLDNVV
                     TSSLVFEGRGRIGEYVGGYSDWLRQRRPAAEEKATAKPAATIKTATPAPAAAPAATAT
                     PAPAKKPAKLGYKEQRELDALPAQIEALEAEQAELGTRTAAAGFYQQPQAAQAAVLDR
                     LAALERELEAAYARWDALEALKDGR"
     gene            16700..17554
                     /locus_tag="PK144_02325"
     CDS             16700..17554
                     /locus_tag="PK144_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF64591.1"
                     /translation="MPHTTDHLLLDDGQCLRVVRRGTQAGGRPVVCLHGWTSTHADWT
                     HWTQALAGAHPSFAWDARAHADVPLTGAPATLERMADDLDALIRHYALERPLLLGHSM
                     GALTIWMHALRHPGTAIGGYGLIDQTPKLVTGAGWRFGIYGDFSAQRNRWFIARQRED
                     FAEAVLELTADGLNARARERYEREPAKFQRSRDWLAGLAHAALTECWCSLAGADLRPA
                     LARLHAPVLLVYGDESNFYTAETARWVQAQIPHARLETYAGADHCPHFWQRDRFLGEL
                     RALLAACG"
     gene            17742..18515
                     /locus_tag="PK144_02330"
     CDS             17742..18515
                     /locus_tag="PK144_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF64592.1"
                     /translation="MPPPLTILWRDARFVAVDKPAGLLMHRSPIAERVEDFLLQRLRD
                     QLRRHVYLIHRLDRPTSGVVLFALDPEAARAACAHWEERTVEKSYLAVVRGWPAETGT
                     IDYALAEEPGKPLQPAITHWRRLATAEIPVPVGRYAQARCALLEVRPETGRLHQIRKH
                     FHHVSHHLIGDTTHGDGRHNRLFRERFGCRRLLLHAWRLALPHPLDGTPLELIAPPDA
                     ELAQLFDALGGSPGNGPGAGLRQDQGLAGWQASIPYGCV"
     gene            18662..18943
                     /locus_tag="PK144_02335"
     CDS             18662..18943
                     /locus_tag="PK144_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64593.1"
                     /translation="MRTTTAIALGFSATLGLAALAQAGDFEQYPPTDGWGRPVAAADA
                     ALQESAEMQIRMMPGYAQGDYLPAEGPTCDLAQTLFPVHSTEAGSAGLC"
     gene            complement(19055..20512)
                     /locus_tag="PK144_02340"
     CDS             complement(19055..20512)
                     /locus_tag="PK144_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HNF64594.1"
                     /translation="MHPIRMPLALVLALASAAAPAAPVLVNALAIAGDATDLSGLPAG
                     PNGNRLGGFASDLHYDRYSNTYYGIPDRGPGGGLLAYDTRVQRFTLNVDSATGAISNF
                     QLQSTTMLTAGGQPLNGLNPALLNASSATLGLSQDPEGFAVGPHGTYFVSDEYGPAVR
                     EFSAGGELIRSFTTPANLIPRDSGGGVNYVDGRPVIVSGRQDNRGFEGLTLSPDGTKL
                     YAMLQDPLVDEGSSSDGRRSRNLRLVEFDVATGLATKQMIYRLEDRDDINARIPGTAD
                     DFSATNQGRSIGISSITALNDHEFLVIERDNRGIGEADPTGTAPVGSKRVYRIDISGA
                     SDVSSTSLAGDNALPPGVVPVTKSLFLDVQAALLAAGLPIPEKLEGLTIGPLLDNGFI
                     DLILGTDNDFSVTQSGSGEQFNVCTGPGGTFRDVALDQACPGATALLPTYLYAVRVEL
                     AGLNYVAPLTVAEPFGLTLFGLGLAGLAARRRCRA"
     gene            20740..>21766
                     /gene="aroC"
                     /locus_tag="PK144_02345"
     CDS             20740..>21766
                     /gene="aroC"
                     /locus_tag="PK144_02345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNF64595.1"
                     /translation="MSGNTYGKLFTVTTAGESHGAALVGIVDGCPPGLELSEADLQVD
                     LDRRKPGTSRHTTQRREDDVVRILAGVFEGRTTGCPIALLIENTDQRSKDYSKIADRF
                     RPGHADYTFHHKFGLRDHRGGGRSSARETAMRVAAAGIAKKWLRARHGVEVRGYLSQL
                     GPLRAQALDWNEVGNNPFFCPDAALVPQLESYMDALRKSGDSVGARVTVVASGVPVGL
                     GEPIFDRLDAELAHALMSINAVKGVEIGDGFGCVEALGTQFRDELTPAGFLSNHAGGV
                     LGGISTGQDIVAHIALKPTSSIRLPGRSIDVDGNPVEVITTGRHDPCVGIRATPIAEA
                     MVALVLMD"
BASE COUNT         3126 a         7842 c         7494 g         3304 t
ORIGIN      
        1 acgccggtct acatgatcag cgagaaggcg agcgacgtga tcctggagga cgcagcggca
       61 aactgaagcg ctgcggcaat tccggtccgg cagtcccggg cggacgccgg atcaggccac
      121 cgaatgaatt cgcatgcccg cgtgtcttca atgactccgg gcacgccgag gcgtcatcga
      181 acctccggag gcagcatggt cagcaagggt ttactcgtgc gactggaagc gagacacggc
      241 agacaggccg aggtggaaga ctttttgtgc tcggcgctgc cggccgtacg tcaggaagcg
      301 ggcacgatcg cgtggttcgc cctgcgcttc gggcgcgggg agtacggcat ccttgatgtc
      361 ttcgccgatg acgccggacg ggaggcgcac ctcgcgggcg tcgtggccca agccctgatg
      421 gcgcaggccg aagtcctgct gtcggagccg ccgcgcatcc agcgcctgga cgtgctcgcc
      481 gacaagctgc ccacgctcca gccggccgag cccgacacca aggccctgct gctgagcttc
      541 ctgccgaagt caggccatgc agcgcaggtc gagcagttcc tgcgcgacgc gcaaccgatg
      601 gtgcaggaag aacccggcac gacggcgtgg ttcgctctgc atttcgacga tggccattac
      661 ggcatttttg acacgttccc ggacaacggt gcccgcctgc atcatctgac cggccgcgtc
      721 ccgcgcgaac tcgcgaagca cgcgctgtcg ctgctcggca gcctcccaga ccccgaactg
      781 cccggcgtgc tcgcggaaaa actcggcgaa tgagccggct caacgccgat caagcgggaa
      841 tgcgaccatg actgaaaccg aattcctgaa tctcgtccac cagggcgccg gccgcggcag
      901 tttcgaggaa ggctgggaga gcggcggtgc ctgggaaatc cacgcgcagg tcgtcatcgc
      961 cgcgttcctg cgcagcggct acggcatcac cgatgcccgc gaactggcgt atccgggctc
     1021 gcaggagcac tgcgatttcg gcttcacgca caagggccgc aagtacgcgg tcgagttgaa
     1081 ggtcgagaac aagaaggacg gcaagttcgg cggcatgtcg ctcgatcagg cgatgctgac
     1141 cgacgtgaac aagctgcacg cgttcaacgc cgacgaactc tggttcctcg tcatcgcccg
     1201 ttcggacaat gccaagcagc ggctgctcga actcgccatg cgcggggatt cgtggatcgt
     1261 cgatcaggag gccggctttc tcgcggcgct ctgcaacatc cggacgcagc cgcacggact
     1321 gccgtggcag cgctatgaga aacaggcgct gaagctggag cagtcgccgt tttaccagtg
     1381 aggggcaccg gtcgccggca tggtgcatgc cggcgaccgc aatccgcgca ggatcgccgg
     1441 atcgtctccg gcttcacgca cgcttccaca cgactcccgc acgcgccgtc ctgagttcgc
     1501 cgccgtgacg cctcctgccg cagcgatcct ccccggcccg caaacccgcc gatacgtcgc
     1561 tgcacgcccc tcatgccgga gcatggcttg ccggccgcgg ccggcccgcc catgctgcgc
     1621 ccccttacgc accgagcccc gccgccatgt ccgcgtcgtc cgaaaccgtc gccagccccg
     1681 cgccggggct gcatcccgag tttgccaccg gcgcgctgta tgccgtgctc gcctacctga
     1741 cctgggggct gatgccggcc tactggaagc tggtgccgga gctgtcggcg ccggagctga
     1801 tcgcgcaccg cgtcgtctgg tcggtgttcg tgaccggggc gctggtgctg gccttccggc
     1861 gcgggcggga gctgcgcgcg acgctcaccg acacgcgccg gctcggcgtg ctgctcgtca
     1921 ccgcgctgct ggtcagtgtc aactggctgg tgttcgtgtg ggcggtgctg aacgggcacg
     1981 tgctcgaatc gagcctcggc tacttcatca atccgctgtt caacgtgctg ctcggcgtgc
     2041 tgctgctgaa ggagcggctg cggccgctgc aggggctcgc ggtggcgctc gccgcggccg
     2101 gggtgctgaa ccagggctgg cagctgcagg cactgccgtg gatctcgctg gcgctggcgg
     2161 cgagcttcgg tgcctacggg ctgatccgca agctggcgcc gatcgagccg ctggtgggct
     2221 tgacggtcga aaccctgctg ctcgcgccgc tcgccctcgc cggcctgctg tggctcgatg
     2281 cgcagggcct cggccacttc ggccacggca cgccggcacg cgacctgatc atcatcggtg
     2341 ccgggctgat cacggcgctg ccgctgctgt ggttcgcgaa cgccgcgaag cgcctgcggt
     2401 tttcgacgct cggcttcttc cagtatctgg cgccgagcgt gcagctgatg ctcgccgtgc
     2461 tcgcctacgg cgaggccttc acggccgtgc atgccgtgac cttcggctgc atctgggccg
     2521 cgctggcgct gtacacctgg gacggccgcc gggcgctgcg catcgcctga accggccgac
     2581 gcgcagcgcc ttgctgcgtc gggctcagcg ctgcgggttc ggcgattcgc gctcttcggc
     2641 cgtgtacggt tcggcgacgc gcacgcggaa gaacaccggc tcctcctcgc cctcgccgat
     2701 ggtcacgagc ccgaccttgg tgacggtctc gcccggcccg agatccggga cttcctcgac
     2761 gtgctcgccg gtgccgccga tgcgcgccga gccgatgtgc acgccgtggc ggtcgaacag
     2821 gtagatgttc acgcgcggca gcaccatctg cagcgtgtcg ttgctgagcg tcaggtggta
     2881 ctcgtagccg gcgcgcttgc cgcgcttgac cagcgtgaac atgacgttgc gcaggtagta
     2941 gtcgtcgatc ggcacgacct gatcgagcac gagctggcgc aggcccggca ggcgcttggc
     3001 gatcagttcg gtgacatcgg cgcgcaggcg ttcgagttcg ggccgcacct gcgtgagctc
     3061 ggcatcgcgc agctcgttct ggtggcgcag ctcgtcgttt tcctcggaca gcgacagcat
     3121 gcgcagcgac agcacgaggc tgatgaacac cgtcagcatc aggctcgcga ccagcgccgc
     3181 gaacagggcg cggctgcgca ggctccagcg gtggttgcgc ttgcgggtgc gcacgcggct
     3241 gcgcaccgag cgcacccggg tttcgtcgcg tgccgtgcga tcgtcatcga actccagttc
     3301 gacttcgttg gccgtgatga ccggattcac gttgtctttc tcccccgtat cccgcccgcg
     3361 gtccgtggcc gacggttcat gttcatcatc gtcatcgcgc agcgatcgct gtcgccggat
     3421 gtcgtcgtcg attcgttgca accagtgcat cggtcctccc cccgtcacgg gtccgtccct
     3481 ggcccgcaaa ccgctctgcg gcagtacccc ggcatgatgg acgcaacgct gaggaatcga
     3541 tgatgaacat cgtttcacgt ggatacggca cgccgcggcg gcgcgcatga tccatgccga
     3601 cccgagcgct ctgcaagtac accccggaga gccccccggc gccgctgccc gaagccgaac
     3661 cggaaccgac catggcccga tccgcccccg ccgcctgtgg tgcccgaacc gtcgatgcga
     3721 tcctggacgc cacgctcgcg ctgctcgatc gcgacggcga gcggcgcacg accacgaacc
     3781 gcatcgccgc cgcggccggc atcagtccgg gcaacctgta ctaccacttc cgccaccgcg
     3841 aggcgatcgt gctggcgctg ttcgaccggc tggtgcaggc gctcgcggcg cggcgcgccg
     3901 gaatcgaccg tcacccggcc gccgcagcgt tcgcggcaga cttcggggca ctggtgcaga
     3961 cggtgcaggc gcgccccggc ctgttccggg cgctgccgga gctgctcggg caggatgcgg
     4021 aactcaccgg acgctcgcac gactgggccg cggcggaaca ggggcggctg atccggcatt
     4081 tcgcggccct ggcagcggcc ggcctcggca cgccgccgcc ggcgctgcag gcgccgctgg
     4141 cggcggccgt atggcatctg ctgctggccc gcgcctggcg gggagccgag gcaagcgaac
     4201 cgctgccgtc cgcgatcctg ctggcaccgc tcgccgcctg gctggatgcg ccgacgctgg
     4261 cgcgtctggg cgcggggatc ggggatgctg cttaccagtc gccccagtcg tcctcttcga
     4321 gcccgtcgga ttcctcgtag agcagcgcca ggcgctcggg cacgatctcc tgcaccttca
     4381 gcgcctcggc gcgcggcacc agcgcgaagt cgaagttctt cgcgtccatg cgcacgatgc
     4441 cgatctgccc gagcgcgatc tggcggcgct gcgcggcggt gacgcgcacg ctgcgcacga
     4501 agcgcccgtc cgggaagttg tagcgatcct cggccctggc gtcattgagg cggtgcgcct
     4561 cgaccagttg cctgacgcgc gcgaggttgg cctgacgctg tttttcggcc tcgcgcttcg
     4621 cgttcagctc gcggtcgcgc tggcgcttgg cctcggcttc ggcctgacgc tggcgctcgg
     4681 cttcgagctt ctgcgcatcg cgctggctcg gcgactgctg cttctgctgc tggtgcttct
     4741 gctgcttgcg gtccttctcg gccttctgca cggcatcggc actgaccagg ccggccttca
     4801 gcaactgatc tcgcaaggac atggtcacga ctcccggtac ggatgcgggt actgacggat
     4861 cgggtacgga tccggatgct caatgcccct ggcgggccag ccagccggcg acgcggcggg
     4921 cgacacggtc ccagccgggt tcgaacatca tcacatgggc gacgcgcggc acgatctcgc
     4981 actcggcgcc gagccggcgc gcggtttcga gcacttccga gggcggcacc agcgcatcgt
     5041 gctcggcgcc gagcaccagc agcggcgtgc gccgcaccag cgtcaccgcc ggcaggtcgg
     5101 cccaggtcag gtcggcgagc acgcgcgagg attcctcctg cgcgcggttc atcagccggt
     5161 cgacgacggc atcgggtgca tcgggcgaga acagcgcgcg ccgggcgagc gagcggctgc
     5221 cgtactgcgg accggcgtgc gtgatgacgc ccatctccat ccacagcatc ggatcgcgcc
     5281 acatcagttg caggctcgac cacatcagcc ccgagggcgg caccgagcac atcagcaccg
     5341 ccgccggcgc cggcgtgcgc tccagatact tctgcacgac catgccgccc atcgaatgac
     5401 cgatcaggat cgtgcgctcg ccgaggccga cggcgacctc ggcgacatcg gtcacgtagt
     5461 cggcgagccg gttccagtgc aggcgatcgc gcccggcgct gccggcatgg ccgcgcagcg
     5521 acagcgcgta ggacgggtag ccgagaccgg cgaagaaatc gagaaagtgt tcctcccagc
     5581 accaggcacc ggcgaaggcg ccgtgcacga acagcagggg gggcttgtcg ccggcctgcg
     5641 cgggcaggcg gctgatgact tccagttcga gaggcgcggc ggcgctgcct agaaacacgg
     5701 tgcgtaacct cgggcgcgga tgggggaatc ggtcggacag acgcgcagac agcaaggcag
     5761 cgcgcgggca tgcgcaggcg caggccgcgt ccggacaatc ggtaacgcag acggcgggcg
     5821 gctgcacgtc aacatcggtc aggacaccgg gatttgcgcg ggcggcgacg gggggcggcg
     5881 tcgggacgga gccggcgggg cctcagtaca ggatgcgcgt gcgcagcgtg ccgtcgatcg
     5941 cgcgcagctc cttcagcgcc agcttgccgg cgtgctcgtc gtcgtcgatg tcgatgacca
     6001 cgtagccgat gccgccgcgg gtctgcaggt actggccggc gatgttgatg cgctggcgtg
     6061 acagcgcatc gttgatcgcc gacagcaggc cggggcggtt ctcgtggatg tgcagcagcc
     6121 ggcgcttgcc ggggtgtgcg ggcaggctga cctcggggaa gttcacggcc gacagcgtcg
     6181 agccgttgtc gctgtagcgc accagcttgc cggcgacctc gaagccgatg ttctcctgcg
     6241 cctcctcggt gctgccgccg atgtgcgggg tcagcagcac ctgatcgaag gcgcgcagcg
     6301 gcgacacgaa ctcgtcgtcg ttgccctgcg gctcggccgg gaacacgtcg atcgccgccc
     6361 cggcgacatg gccgctcgcg agcgccgccg ccagcgcgtc gatgtcgacg acggtgccgc
     6421 gcgaggcatt gatcagctgc gcgccggccg gcagccgcgc cagttcggcc gccccgatca
     6481 tgttgcgggt ctgcggcgtc tcgggcacgt gcagcgtcag cacgtcggag cgcgcgagca
     6541 gctcggcaag cgaatgcacc ggctgcgcgc cgccgagcgc gagcttggcc tcgatgtcgt
     6601 agtagtgcac ctgcatgccg aaggcctcgg cgaggatgcc gacctgcgtg ccgatgtggc
     6661 cgtagccgac gatgccgagg cgcttgccgc gcacctcgcg actgccggcg gccgacttcg
     6721 accagccgcc gcggtggcag agcgcgttct tggccgggat gccgcgcatc agcagcacga
     6781 tctcggccat caccagctcg gcgacgctgc gcgtgttcga gaacggcgcg ttgaacaccg
     6841 gcacgccggc gctcgcagcg gcctcgaggg cgacctggtt ggtgccgatg cagaagcagc
     6901 cgacggcgat gagtttcttc gcctcggcga gcacggccgg ggtcagttgc gtgcgcgaac
     6961 ggatgccgac gatgtaggcc tcgcgcaggc gctcgatcag ctcgggttcg ggcagcgcct
     7021 tgcggtgctg ttcgaggttg gtgtaaccgg cggcgcggaa ggtctcgacc gcactcggcg
     7081 cgatgccttc gagcagcaga atcctgattt cgcgtcggtc gagggaaacc ttgcccatgg
     7141 tcggatgctc ctcggattgc cggcagacgc ggccgggccg gcgatgatgt cgcaaaatgc
     7201 cggtggccgg aaggccgggc agcggcccgg ccctcgccta cgctgcacca gcgcagcctc
     7261 tgtaaccctg ccggagaccg ccatgtccga tgccgccatc ctcgcccgcc tcggcgcgct
     7321 gctgccaccc gcgcgcctgc gcaccgatgc cgacaccctc gccagccacg gccgcgactg
     7381 gacccgcctg taccagcccg acccgctcgc ggtcgccttc ccggaatcgg tcgccgaagt
     7441 gcaggccctc gtcgcgatcg ccaacgacac cgcctgcccg ctcgtgcctt cgggggggcg
     7501 caccggcctc tccggcggcg cggtggcgaa gtcgcgcgag ctggtgctgt ccttcgagcg
     7561 catgaaccgc atcctcggct tcgatgcggt cgatcgcagc gtcaccgccg aacccggcgt
     7621 catcctcgaa accctgcagg actacgcccg cgagcatggc ctgcagtatc cggtcgactt
     7681 cgcggcgcgc ggctcggcgc agctcggcgg cacgatcgcg accaacgccg gcggcatcaa
     7741 ggtgatccgc tggggcatga cccgtgactg ggtcagcggc ctgaaggtcg tcaccggccg
     7801 cggcgagctg ctcgatctga accgcggcct ggtcaagaac aacaccggct acgacctgcg
     7861 ccagctgttc atcggctccg aaggcacgct cgggctgatc gtcgaggcca cggtgcgcct
     7921 cgccgaagca ccgcaggcac cggcggtcat ggtgctcggc ctgcccgacc tcgaagcgat
     7981 catgagcgcc ttcgagcgcc tgcgcgccgc gctgcggctg acggccttcg aatgcttctc
     8041 ggcgcaggcg ctttcctacg tcaccgccca cggccagccg cggccgttcg agaccgaaac
     8101 gccgtggtac gtgctcgccg agtacgacgc cgccgaagag cgcgacaccg aagccgcgat
     8161 ggcggccttc gagacgctcg ccgaggacgg catcgtgctc gatggcgtgc tgtcgcagag
     8221 cgacagccag tggcgggcgc tgtggcgcct gcgcgaggac atcaccgagt cgatcgcgca
     8281 ccgcctgccg tacaagaacg acgtctcggt gcgcgtcgcg cgcgtgccgg aattcctgcg
     8341 cgcgatggat gaactcttcg cgcgcgaata tccggacttc gaggtgatct ggttcggcca
     8401 cgtcggcgac ggcaacctgc acatctcggt gctgcagccg ccggaaatgg cacgcgccga
     8461 cttcgtcgcc gcctgcgagc aggtcagcga gcacctgttc gcgacgctcg ccgcgctcgg
     8521 cggcagcatc tcggccgagc acggcgtcgg gctggtcaag aagccctacc tgtcctacag
     8581 ccgcgatccg gccgagatca gctatctgcg ggcgatccgc gcggccttcg atccgaacgg
     8641 catcctgaac ccgggcaagc tgttcgatcc ggcctgacat gccccgggcc ggtcgcgttt
     8701 gcgccatgcg ggaagcgccg gtatcctcgg cgcctcgccc gcgcagccga ccccccccgc
     8761 gtccgtacgg ataccccgcc cgagccgtcc cgcttgctgc ccccctgccc gctgcggctg
     8821 ccccgcacat gctggccgac cgttcccggc cgccggcgtg accgccgatg atgacgcccg
     8881 acgcgctgcc acgctcgccg ctctgtcgct atctcgcccg cgcgctgctg ctgctgctgg
     8941 tgccgctggt gctgctggtg ttccaggact acggcgtcag caacgacgaa ctggtgcaga
     9001 gcacctacgg caagctgctg ctcgattact accgcagcgg cttcagcaac gacgcggcgc
     9061 tgcactacct cgacctgtac cgctacggcg gcgccttcga catgatcgcg gcgctgctcg
     9121 tgccgctgtc gccgttcggg gaatacgaga cccgccacct gctcggcggc ctgctcggcc
     9181 tcgtcggcct gctcggcgcc tggcggctcg gccggctgct cggcggcgat cgcgccgggc
     9241 tgctcgcact cgtgctgctg ctgctgacgc cgtgcttcat cggccacagc ttcaccaacc
     9301 ccaaggacgc gccgtttgcg accgcgatgc tgtgggccgt ctacgcgatc gtgcgcgtgc
     9361 agcacgaact gccgcggccc tcggcgcaca cgctcacgct gctcggcgtc accatcgggc
     9421 ttgcgctcag cgtgcgcgtc ggcgcgatcc tgctcgggcc gtatctggcg ctcggcgtgc
     9481 tgctgtggct ggccgggcgc tggcgtgccg gcgagcggcc ggcgcgactc gccgccgatg
     9541 ccgcgcgcgt ggcgctgcgc tgcgccccgg cgttcgtgct cgcctacgtg gtcatgggcg
     9601 tgttctggcc ctacgcctgg cagtcgccgc tgaacccgct gaagacgctc ggcgagttct
     9661 cgcacgtcgc cttcccgatc gagacgctcg ctgccggcga gtggtatccg gtcaacgcca
     9721 tcccgcgctg gtacgtgccg gcctacctgg tgttcacgct gccggagatc gtgctggccg
     9781 gcctggcctc ggggctggcc ttcgcgctgc tgcgcctgcg ccaccgctgg ccgatgtccg
     9841 cacgcgccca gtcgctgtac gtgctcggca ccgccggcgt gctgccggtg ctgatcgcgg
     9901 tcgccctgca caccacggcc tacaacggcc tgcgccattt cctgttcgtc gtgccgccgc
     9961 tggtggtctg cgcggcgctg gcgctggagc gcatgcacga gatgctcgcc gcgcgcgcgc
    10021 cgctgcacgg ccgcctgcac acgctcgcgc tcgccgcgct gctggccgtg caggccggca
    10081 tcctcggcta cctgcacccg caccagtaca tctattacaa cgtgctgatc ggcggcgtgc
    10141 cgggcgcggc cgatcgctgg gagctcgatt actggggcaa ctccctgccc gaggccatcg
    10201 acggcctcgc cgaatacctg accaacgagt acgagggcgc gccgatcgcc cgccgctaca
    10261 gggtcgcggt ctgcggcaag gaagaaagcg cccggtatta cttcccggat ttcctggtgt
    10321 tcgagccgcg ctgggagagc gccgacttcc tgatcgcgct gaccaacgac ggctgcgacg
    10381 aacagctgcg cctgccgaac gccgtcgaga tcgaccgctt cggcgtaccg ctgtccgtgg
    10441 tcaaggacct gcgcggctac gtgctcgaat acaacccggc cttcaagccg caccgctccc
    10501 gcccgaagtt cctgctctac ctgcgccgct acggcaagcc ctagcccgtc gaggtcccga
    10561 ctcccgtgta cgacgcatcc gtcttccgcc gtccggcgtt cgtcgccgtc gcgctcgcga
    10621 cgctcactgc cgccctggtc tggtggtatg ccgcccgccc ggtcgacatc ggcatcgact
    10681 ggcacgaagg cacctggaac agcgcctcgt tcgcaccgtt tcgcgccggg cagagcccgc
    10741 tgaccaggaa ctggccgacg cccgaacagg tcgagaacga tctcgcccac gtgaagaccg
    10801 cctttcgcgg cgtgcgcacc tacgccgcca tcggcgccgc gcaggacgtg ccggcgctcg
    10861 ccgcccgcca cggcctcaag gtcatccacg gggcctggct cagcagcacc ccggccgaca
    10921 acgaggccga ggtcagcgcg ctgatcgagg ccgccaaccg ctatccgcag tcggtcgagc
    10981 gcgtgatcgt cggcaacgag gtgctgctgc gccgcgacct cgacgccggg cagatcatcc
    11041 ggcacctcga ccgcgtgcgg cagtcgatcc gccagccggt gtcctacgcc gatgtctggg
    11101 ccttctggct gaagaatccg cagctcgccg accatgtcga ctacctcacc atccacatcc
    11161 tgccgtactg ggaggacgag ccggcctcgg tcaccgacat ggagcagcgc ttcgtcgagg
    11221 tcatcggcaa gatcaaggcc gccttcccgg gcaagccgat cctgatcggc gaggccggct
    11281 ggccgaccga aggccgctcg cgcggcccgg cgatcgccga cctgccgaac gccgcgcact
    11341 tcctgcgccg gctgccgcag ctcgccgcgc agtacggctt cgattacaac gtcgtcgagg
    11401 cctacgacca gctctggaag gcgcagcagg aaggcacggt cggcgcgcgc tggggcctgt
    11461 acgacgtcga tcgccagcag aaattcggca ccaccggtcc ggtcgatccg ttcccggaag
    11521 ccgggctgcg cgccggcgcc tcggtgctcg gcgggctggc gctggcgctt tggctgatcc
    11581 cggccggctt cggcctcacg cgcgcactcg ccgtcgccgg gcttgcacag ctgctcgccg
    11641 ccggtgcgat cgacaccctc tggcatgccc agcgcctggc gatctcgccg gcctcggtga
    11701 gctggtgcct gcagcggctg ctgtttttcg ccgccgacac cggcctgctc gacaagccga
    11761 ccgtcagcga gctgtatcgc ggctacatgg acacgacgcc ggcgctcgcc gaagcctggg
    11821 cctgggtgcg catcctcgcc accgtcgtcg ccaccggcgt gctggccgcg ctgctgcgcg
    11881 cccggctgtt cggccgcgag gccccgggcc tggcgcgcgc cgcgcgcctc ggttacctca
    11941 gctacgtcgc cggtgcgctg atgatcagcg cgatgctggc cttcaacggc cgctacctcg
    12001 acatcccgat cccggaattc tggctgccgg cgctcggcct gccggcgctg tggctggcgg
    12061 cgcgcctcgc aggcagcccg cgcggctacg gactggccgc cctgaccgca ccggggccgg
    12121 cgcgcctgaa gcttggcctg tggctgctcg ccggcgccgt cgccgtgatc ctcggcgagg
    12181 gctttgccat cgtcggcgag gacttcacgg ccatgcaccc ggactggtcg gagcgcgcgc
    12241 cgctgatctg gcacgcgttc ttctggaacc ggcagatcct cgcctgcgcg ctgatgctgg
    12301 tctggctggc gctgccgctg ctcgctgaat ggcgccggcc ggtcgcggcc gcgacctgag
    12361 gccgtcgcga tgcaccagac cgcccggcag ctgctgcgct tcggagccgt cggcatcgcc
    12421 ggactcgtga tcgacgtggc ggtgctcgaa gccgcactcg cgctcggcgc cggcttctac
    12481 ggcggccggg cgctgtcctt cctcgccgcg gcaagcggca cctggctgct gaaccggctc
    12541 tggaccttcc gcagcgaaac cgcacgtgcc aaccgcgggc tggccggcga atgggcgcgc
    12601 tacgtggcgc tgatgctggc cggcggcagc gtcaactacg cggtgtacgc gctctgcatc
    12661 gaaacctcgg cgcaggcgcg cacgtggccg gcgctggcgg tcgccgccgg ttcgctggcc
    12721 ggcatggtcg tcaactacgc cagcgcccgc tggctggtgt ttcgcagcgg cacggcctga
    12781 aagccgctgc gccccttccc gcgtcaggcc tcgcgcagtt ccttcccggt cagctccagg
    12841 aacagatcct ccagcgtgcg cgggcggatc gacaggcgct cgagcgggac gccggcggcg
    12901 agcaggtcgc tcagtgcggt gctgacgttg cgcaccgaga tctcggccga ttcgcgccgc
    12961 cgctgcacgc tgtaggccgg cggcagcgcg cggccttcga ggtcttcggc cggcagctgc
    13021 aggatgcgat cgtcgaagtg ctgcgcgagc agcgcatcgg ggctgccctc ggcgacgatg
    13081 cggccgcggt ccatgatcgc cagcgaatcg cagagctggt aggcctcctc catgtagtgc
    13141 gtggtcagca ccacggtctt gccgcggcgc ttgatgtcct cgaccagctg ccagaagttg
    13201 cgccgcgcct gcggatcgag gccggtggtc ggttcgtcga gaaagacgat gtccggatcg
    13261 ttgaccagcg ccaccgcgag cagcagacgc tggcgctggc cgccggagag cttgcggtgg
    13321 tcgcgatcga ggaagtcctc cagcgcgcag gcgcggatca gctctgcggc cggcacgcgg
    13381 cgcggataca gctgcgagaa gaagcgcagg cactcgcgca cggtcaggaa atcctgcagc
    13441 gccgtgctct ggaacatgat cccggcttcc tcgcgaaagc gcaggccgag cggctcgccc
    13501 ttgtagagga tgcgcccggc gctcggcgtg ctgatgcctt ccatcatctc gatggtcgtg
    13561 gtcttgccgg cgccgttcgg gccgagcagg ccgaagcagc gcccgggcag gatcgagaag
    13621 ctcacgccat cgacggcgcg caggccgggg tactgcttga ccagctcgcg cacttcgatc
    13681 aggggggcgg acgtcatggg cgggcgcact cgggcgaaag gacagggacg ggcggggcga
    13741 accggttcac ggtacctgcg acaggatcgc cagcgcctcg gcgtgcacgc gcgcatcgcc
    13801 ggcgaccagg atgcggccgc cgtgcgcggc gttctcgccg cgccagccgg tcatcaggcc
    13861 gccggcggcc tcgacgatcg ggatcagcgc ctgcacgtcg tagggcttga ggccggcttc
    13921 gacgacgaga tcgagacagc cggcggcgag catgcagtag gcgtagcagt cgccggaaaa
    13981 gcgcgtcatg cgcacgcgcg cggcgatggc atcgaaggcc ggcttctcgt gcccgcgctc
    14041 gaagagatcc ggatgcgtcg agtacagcgc cgcatcggcg agccgcgcgc aggcccgcgt
    14101 gcgcagcggc cggcggccgt cgcgatcgcg cagttcggcc ccgaagcgcg agccgatgaa
    14161 gcgctcgccg gtccacggct ggtccatgac cccgagcacc gggcgctcgc cgtcgtagag
    14221 cgcgatcagc gtgccccaga gcggcatgcc ggtgatgaag gcgcgcgtgc cgtcgatcgg
    14281 gtcgatgacc caggtgaggc cggagtcgcc gggctcgaag ccgtgctcct cgccgtagcg
    14341 cccgtcctgc ggccggcgct cggcgagcag cgcgcggatc gcggcctcgg cgtcgcggtc
    14401 ggcgaccgtc accgggtcgt acatcgtgcc ttcggcaccg gacttgtcgg cgaccgcgag
    14461 cggggcgcgg aaatgccggc ggatgacctc acccgaggcg tcggcgagca gatgggcgag
    14521 cgcgatcagt tcgccgagct gcttcggtgc cagctcggcc ggggcgtggc gcggcagcgc
    14581 cggggcggtc ggactcatgc gggacgatcc tcggacgacg atgcagacga ttacggacgt
    14641 aacgcgggcg cgcagcttag ccgatgccgg ccggcaccgg ccgatgagca cggcgatgcg
    14701 gctacaatcg cgcccccgcc ccgccctgcg tgtcaccgcc atgccgctcc tgaccttcga
    14761 tcgcgtttcc gtcgccttcg gcctcgccgc gctgctcgac aacgccgatt tccagatcga
    14821 tgccggcgag cgcgtctgcc tgatcggccg caacggcgcc ggcaagtcga cgctgctgaa
    14881 gctcgtcgac ggcacgctcg cgcccgatgg cggcgaactc tggcgccagc cgggcctgaa
    14941 gatcgcgcgc ctcgaacagg agatgcccga gaccggcgac gccaccgtgt tcgagatcgt
    15001 cgccgacggc gtgcccgagg tcggccgcct gctcggcgaa taccacgctg ccgcccacgc
    15061 cctcgcgcac gatcacgacg ccgcacacgc cgatgccctg ctcaggcgca tggagcgcct
    15121 gcagcacgaa ctcgaagccc gcgatggctg gagcctgaac cagcgcgtcg agcagatcct
    15181 gacgcgcctt gcgctgccgg ccgacgtgac gctcgccgag ctctccggtg gctggcgccg
    15241 gcgcgtgctg ctcgggcggg cactggtctg cgagcccgac ctgctgctgc tcgacgagcc
    15301 gaccaaccac ctcgatgtcg aggcgatcca gtggctggaa gaacacctgc tcggcttccg
    15361 cggcggcgtg ctgttcgtga cccatgaccg cgcgctgctg acgcggctgg cgacgcgcat
    15421 cgtcgaactc gatcgcgggc ggctcagctc ctggcccggc gactatccga acttcctcga
    15481 aaggaaggcc gccgcgctcg aggaggaagc ccgccacaac gcgctgttcg acaagaagct
    15541 cgcgcaggag gaggcctgga tccgccaggg catcaaggcc cgacgcacgc gcaacgaagg
    15601 ccgcgtgcgc gcgctgaagg cgctgcgcga ggaacgctcc gagcggcgcg aactgcaggg
    15661 cagggtcagc ttcaacctcg aagaggccgc ggcctccggc aagctggtgg tcgaggcgaa
    15721 ggacgtgagc tacgcgtggg acggcgcgcc ggtcgtgcgc gatttctcgg tgcgcgtgct
    15781 gcgcggcgac cgcatcggcc tgatcggccc gaacggcgcc ggcaagacca cgctgctgaa
    15841 cctgctgctc gggcggcttg cgccggacag cggctcgatc cgcctcggca ccaagctgca
    15901 ggtcgcgtac ttcgaccagc tgcgcgcggt gctcgacccc gaatcttcgg tcatcgacaa
    15961 cctcgccgag ggtcgcgagt tcatcgagat caacggcgaa cgccgccacg tcatcagcta
    16021 tctgcaggac ttcttattca gccccgagcg cacgcgcaca ccggtgaagt cgctctccgg
    16081 cggcgagcgc aaccgcctgc tgctcgcacg cctgttcagc cagccggcca acctgctggt
    16141 catggacgag ccgaccaacg acctcgacat cgagacgctc gaactgctcg aagagctgct
    16201 gctcgactat cagggcacgc tgctgctggt ctcgcacgat cgcgccttcc tcgacaacgt
    16261 cgtcaccagc tcgctggtgt tcgaaggccg gggccgcatc ggcgaatacg tcggcggcta
    16321 cagcgactgg ctgcgccagc gccggccggc ggccgaagag aaggccaccg cgaagccggc
    16381 cgcaacgatc aagaccgcaa cgccggcccc ggccgccgcg ccggctgcca ccgccacacc
    16441 ggctccggcg aagaaaccgg cgaagctcgg ctacaaggaa cagcgcgaac tcgacgcgct
    16501 gccggcgcag atcgaagcgc tcgaagccga gcaggccgaa ctcggcacgc gcacggccgc
    16561 ggccggcttc taccagcagc cgcaggccgc gcaggctgcg gtgctcgatc gcctcgccgc
    16621 gctcgaacgg gaactcgaag ccgcatacgc gcgctgggac gcgctcgaag cgctgaagga
    16681 cggacgctga gccccggcca tgccgcacac gaccgatcac ctgctcctcg acgacggcca
    16741 gtgcctgcgc gtcgtgcgtc gcggcacgca ggccggcggc cggccggtcg tctgcctgca
    16801 cggctggacc tcgacgcacg ccgactggac gcactggacg caggcgctgg ccggcgctca
    16861 cccgagcttc gcgtgggatg cgcgcgcgca cgccgatgtg ccgctcaccg gcgcgccggc
    16921 aacgctcgaa cgcatggccg atgacctcga tgcgctgatc cgccactacg cgctcgaacg
    16981 gccgctgctg ctcgggcatt cgatgggggc gctgacgatc tggatgcacg cgctgcggca
    17041 tcccggcacc gcgatcggcg gctacggcct gatcgaccag acgccgaagc tcgtcaccgg
    17101 tgccggctgg cgcttcggca tctacgggga cttctcggcg cagcgcaacc gctggttcat
    17161 cgcccgccag cgcgaggact tcgccgaggc cgtgctcgaa ctgaccgccg acggcctgaa
    17221 cgcccgcgcc cgcgaacgct acgagcgcga gccggcgaag ttccagcgca gccgcgactg
    17281 gctcgccggc ctcgcgcacg cggcactgac cgagtgctgg tgctcgctcg cgggcgccga
    17341 cctgcgcccg gcgctggcgc ggctgcacgc cccggtgctg ctggtctacg gcgacgagag
    17401 caacttctac acggccgaaa ccgcgcgctg ggtgcaggcg cagatcccgc atgcgcgcct
    17461 cgaaacctac gccggtgccg accactgccc gcacttctgg cagcgcgacc gcttcctcgg
    17521 cgagctgcgc gcgctgctcg cggcctgcgg atgatgcggc gcaataaaat tccggtaatc
    17581 ccgctcgttc cgggaacctt cgccgcggca gccgcgtccc taccttcgct catctcctgc
    17641 aagggctgct gccggggtcc gggtctctcg tacccgcccc ggctcttttt tgcccgcgcc
    17701 cccgcccgtc ccgcggtctg cgatgcgtcc ggaggctgcg catgccgccg ccgctgacga
    17761 tcctctggcg cgatgcgcgc ttcgtcgccg ttgacaagcc cgccggcctg ctgatgcacc
    17821 gcagcccgat cgccgagcgc gtcgaggact tcctgctgca gcgcctgcgc gaccagttgc
    17881 gccggcacgt gtacctgatc caccgcctcg accggccgac ctccggcgtc gtgctgttcg
    17941 cgctcgatcc ggaagccgcg cgcgccgcct gcgcgcactg ggaggagcgc acggtcgaaa
    18001 agagctatct cgccgtcgtg cgcggctggc cggccgagac cggcacgatc gactacgcgc
    18061 tcgccgagga gcccggcaag ccgctgcagc cggcgatcac gcactggcgg cggctcgcca
    18121 cggccgaaat cccggtgccg gtcggccgtt acgcgcaggc gcgctgcgcg ctgctggaag
    18181 tgcgccccga aaccggccgg ctgcaccaga tccgcaagca cttccatcac gtctcgcatc
    18241 atctgatcgg cgacaccacg cacggcgacg gccgccacaa ccggctgttc cgcgagcgct
    18301 tcggctgccg ccggctgctg ctgcacgcct ggcggctggc actgccgcat ccgctcgacg
    18361 gcacgccgct cgaactcatc gccccgcccg atgcggagct tgcgcagctg ttcgatgcgc
    18421 tcggcgggtc gcccgggaac ggaccggggg ccggactgcg gcaggatcaa gggcttgccg
    18481 gatggcaggc ctccattcca tacgggtgcg tttaatggct ctgctgccga taaccaagtc
    18541 ctgcacttgg aaatgcgcag ccgcgggcta cgctgcactt gcacattgca agcgattcat
    18601 ctttccccga cgaatcgccg gcggtgctca cactggcaaa cccgctcagg aggttcgagc
    18661 catgaggacc acgacagcca tcgccctcgg cttctctgcc accctcggtc tggccgcgct
    18721 cgcgcaggcc ggcgacttcg aacagtaccc gccgaccgac ggctggggcc gcccggtcgc
    18781 cgctgccgac gccgcgctgc aggaatccgc cgaaatgcag atccggatga tgcccggcta
    18841 cgcgcagggc gactacctgc cggccgaagg cccgacctgc gatctcgcgc agaccctgtt
    18901 cccggtgcat tcgaccgaag ccggcagcgc cggcctctgc tgaccggggc agccgccccg
    18961 cggcgccggc tgtccgccgc gggacggtgt tcttctcaca aacctgtccg gcagccctgc
    19021 acgaacggcg tcccggtgac gccgttccgc gttttcaggc ccggcaccgg cgacgggccg
    19081 cgagcccggc caggccgagc ccgaacagcg tcagcccgaa cggttccgcc accgtcagcg
    19141 gcgccacgta gttgaggccc gcgagttcga cgcgcaccgc gtacaggtac gtcggcagca
    19201 gcgccgtagc gcccgggcag gcctgatcga gcgccacgtc gcggaaggtg ccgccgggac
    19261 cggtgcagac gttgaactgc tcgcccgaac cgctctgcgt gacgctgaaa tcgttgtcgg
    19321 tgccgaggat caggtcgatg aagccgttgt cgagcagcgg cccgatcgtc agcccttcga
    19381 gcttctcggg gatcggcagg ccggcggcga gcagcgcggc ctgcacgtcg aggaacagcg
    19441 acttcgtgac cggcacgacg ccgggcggca gcgcgttgtc gccggcaagg ctggtgctgc
    19501 tcacgtcgct cgcaccgctg atgtcgatgc ggtacacgcg cttgctcccg accggcgcag
    19561 tcccggtcgg atcggcctcg ccgatgccgc ggttgtcgcg ctcgatgacc aggaactcgt
    19621 gatcgttcag cgccgtgatc gagctgatgc cgatcgagcg gccctgattg gtcgcggaga
    19681 agtcatcggc cgtgccgggg atgcgggcgt tgatgtcgtc gcggtcttcc aggcggtaga
    19741 tcatctgctt cgtcgccagc ccggtcgcga catcgaactc gacgagacgc aggttgcgcg
    19801 agcggcggcc gtcgctgctg ctgccttcat cgaccagcgg gtcctgcagc atcgcgtaca
    19861 gcttcgtgcc gtccggactc agcgtcaggc cctcgaagcc gcggttgtcc tgacggccgc
    19921 tgacgatgac cggccggccg tcgacgtagt tgacgccgcc gccgctgtcg cgcgggatca
    19981 ggttggccgg ggtcgtgaag ctgcggatca gctcgccgcc cgccgagaac tcgcgcacgg
    20041 ccggaccgta ttcgtccgag acgaaatacg tgccgtgcgg accgaccgcg aagccttccg
    20101 ggtcctgcga caggccgagc gttgcactgc tggcgttcag cagcgccgga ttcaggccgt
    20161 tcagcggctg gccgccggcc gtcagcatcg tcgtcgactg cagctggaag ttcgagatcg
    20221 cgccggtggc ggaatcgacg ttcagcgtga agcgctgcac gcgcgtgtcg taggcgagca
    20281 gaccgccgcc ggggccgcgg tccggaatgc cgtaataggt gttcgagtag cggtcgtaat
    20341 ggagatcgga cgcgaaaccg ccgagccggt tgccgttcgg accggccggc agcccggaga
    20401 gatcggtggc atcgccggcg atggcgagcg cattgacgag gacgggtgcc gcgggcgcgg
    20461 cggcggaagc cagcgcaagc acgagcgcca gcggcatacg gatcggatgc atggacaggg
    20521 ttctctgcag caagggggat ggcggcatcc ctgccgtcac ggccgcttcc cgacggcgat
    20581 ctcctgttcc tgcgcgcagt ctggtgcggg tgcatgacgg tttcgtggca gtgatacgac
    20641 accggaaaac gccgccggag ccgcaaaaca cttggcacgc gccgcaccgg ccgcggacaa
    20701 ttgccggctt tcccgagccc cagcccgagc gcgtccgcca tgtccggaaa cacctacggc
    20761 aagctgttca ccgtcaccac ggccggcgaa tcgcacggcg ccgcgctggt cggcatcgtc
    20821 gatggctgcc cgcccgggct ggagctgtcc gaggccgacc tgcaggtcga tctcgaccgc
    20881 cgcaagcccg gcaccagccg gcacacgacg cagcggcgcg aggacgatgt cgtgcgcatc
    20941 ctcgccggcg tgttcgaggg ccgcaccacc ggctgcccga tcgcgctgct gatcgagaac
    21001 accgaccagc gctcgaagga ctactcgaag atcgccgacc gcttccgccc cggccacgcc
    21061 gattacacct tccaccacaa gttcgggctg cgcgaccacc gcggcggcgg gcgctcgtcg
    21121 gcgcgcgaga cggcgatgcg cgtcgccgcc gccggcatcg ccaagaagtg gctgcgcgcg
    21181 cgccacggcg tcgaggtgcg cggctatctg agccagctcg gcccgctgcg cgcgcaggcg
    21241 ctcgactgga acgaggtcgg caacaacccg ttcttctgcc cggatgcggc gctggtgccg
    21301 cagctcgaaa gctacatgga cgcgctgcgc aagtccggcg attcggtcgg tgccagggtc
    21361 acggtggtcg cgagcggcgt gccggtcggc ctcggcgaac cgatcttcga ccggctcgat
    21421 gccgaactcg cgcacgcgct gatgagcatc aatgccgtca agggcgtcga gatcggcgac
    21481 ggcttcggct gcgtcgaggc gctcggcacg cagttccgtg acgagctgac gccggcggga
    21541 tttttgagca accacgccgg cggcgtgctc ggcggcatct cgaccgggca ggacatcgtc
    21601 gcgcacatcg cgctcaaacc cacctccagc atccgcctgc cggggcgcag catcgatgtc
    21661 gacggcaatc cggtcgaagt catcacgacc ggccgccacg acccctgcgt cggcatccgc
    21721 gccaccccga tcgccgaggc gatggtcgcg ctggtgctga tggacc
//
LOCUS       DAOQIE010000019        21733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1082_length_21733_cov_10.810591, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000019 DAOQIE010000000
VERSION     DAOQIE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 21733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21733
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_21733_cov_10.810591"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="trfA"
                     /locus_tag="PK144_02350"
     CDS             <1..633
                     /gene="trfA"
                     /locus_tag="PK144_02350"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HNF64596.1"
                     /translation="SVEFTPHAFLKAIGWGTSAKEYTRLREHLSRMQATALSVYSRRL
                     GKGCSVSLIRKFEFADEAGQKLVRWRVYIEPEMHILFGGVYYTQLEWEQRMRLGTLAC
                     WLHGLYSSHTQPFPMKVETLKDGCGSAARDLKNFRYRLRRSLEELRQVGFLSHWYIDR
                     TDLVHVQRTAVPTDEALPAATADSDPDTATEPPIPPGADPDAPDGNSKPE"
     gene            complement(1305..1979)
                     /locus_tag="PK144_02355"
     CDS             complement(1305..1979)
                     /locus_tag="PK144_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64597.1"
                     /translation="MSEHLRPIATVDIVLFTLAEPADDDGLQVLIAERAQEPFRGRPA
                     LPGGYVHVDEDADTAGTARRVLAGKLGVQAPYLEQLYSFGSRTRDPRGWSISIAYFAM
                     LPAGRIAALPAGARWQPVATLPVLAFDHADIVARALARVRDKATYSSLPAFLLDESFT
                     LPQLYAVYCRVLGSRPDPASFRRKIEAQGFIVPVPGAFSQDRSSRPAQLYRLAGPELR
                     AFDRTI"
     gene            2139..3065
                     /locus_tag="PK144_02360"
     CDS             2139..3065
                     /locus_tag="PK144_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64598.1"
                     /translation="MGYARWDSDDWSDYAAGQAGRSREEVFTRRSLDPEFDPLNIRRR
                     ESRDSAQNPESNAIIVAFDETGSMGSIPDAFVREGLGTMVQALLDRRPVSDPHVMVMG
                     FGDAWCDRAPLQVTQFEADLRIADQLSRLWLEGGGGGNRFESYNLPWYFAARRTSIDC
                     FEKRGRKGYLFTVGDEPPPPKLLAAHVRAFLGDDLRRDLDNAELLRAAGRMYEIFHVV
                     VEQGSHYRSFPDEVEQGWTRLLGQRVLFLADYRQLAEVIVSAIEVNEGRDPDAVVASW
                     DAAAGTVAKALRRLPAASRRRGLLGYFGRSAR"
     gene            3065..4252
                     /locus_tag="PK144_02365"
     CDS             3065..4252
                     /locus_tag="PK144_02365"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003589773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNF64599.1"
                     /translation="MRALAVIGAGYGDEGKGAATDRLAATLGPRALVVRHNGGAQAGH
                     TVIAPDGRRHVFGHIGSGSFAGVPSYLSEYFVASPILCLRELRALAVLGVQPKLHVDA
                     GCAVTTPYDMLLNQAVEAARGAGRHGSCGVGFGETIERHGHAGFALTVADLRKPVRLR
                     ERLEAIRRDWLPARAAALGLDRLPAVAYDNALSVRFVDDCARFLAAIEVCDAGVLRGR
                     PLIFEGAQGLGLDMDRGAFPHVTRSHTGIRNAVTLTRAAGLDALQVQYATRAYATRHG
                     AGPLAHELPAAPAAAVRDATNRPNDWQGRLRFGWLDLDRLATDIRTDLADAGALAVTA
                     ELIVSCLDQMDEVIPHVADGGLVQVPRERCLAWYAQRLGLRAGYGAYGPGRDAVREWA
                     RAA"
     gene            complement(4385..4654)
                     /locus_tag="PK144_02370"
     CDS             complement(4385..4654)
                     /locus_tag="PK144_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNF64600.1"
                     /translation="MSASHTKPPSATDWARLQAMTDDDIDFSDDPERDAHFLAQAVIL
                     PGPKKQITLRIDADVLGYFREQGRGYQTLINRVLRKYVEAQKNRA"
     gene            complement(4584..4934)
                     /locus_tag="PK144_02375"
     CDS             complement(4584..4934)
                     /locus_tag="PK144_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNF64601.1"
                     /translation="MSDRYQEHGFDDHVEWAWDEQKNASNIEKHGIDFQDLPRVTANP
                     ILIARDTRRDYGEIRWQAIGRIQGITIAFVFTEREPRIVRIISARKASKNERIAYETA
                     IRDRLGPSAGDDGR"
     gene            complement(5180..7402)
                     /gene="paoC"
                     /locus_tag="PK144_02380"
     CDS             complement(5180..7402)
                     /gene="paoC"
                     /locus_tag="PK144_02380"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HNF64602.1"
                     /translation="MKFDTPSAENPTDRLRVVGQPVDRIDGPLKTTGRAPYAYEHHDG
                     FTGGFADGLARPAYGHVLGAGIARGRIRSFDAEAARRAPGVLAVISADNAGPLGQGPR
                     NTAKLLGGPRIEHYHQAIALVVAESFEQARAAARLIRVEYAREAGEFDLAKVRDSGRA
                     PQDEPDRRLGDFAAAFAASAVRIDAVYRTPDQSHAMMEPHASMAAWDGDRLTLWTSNQ
                     MIAWGRADLAKTLGIRPEQIRLLSPYIGGGFGGKLFVRADAVLAALGARAIGRPVKVM
                     LARPLVFNNTTHRPATIQRIRIGATADGHIHAIAHESWCGNLPGGSAETATQQTQLLY
                     AGAHRLSALRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKLGLDPVELRIRNDT
                     PVDPHDPQRRFSQRQLVECLRTGAARFGWAQRNPVPGRTRAGRWLLGHGVAAAFRNNL
                     VMKSGARVRLGRDARVTVETDMTDIGTGSYTVIAQTAAELLGVPLAQVDVRLGDSDFP
                     VSAGSGGQWGGNCSTAGVYAACMRLREQIAQRLGFRDAAAVRFADGEVQAGGRVAPLA
                     DAVRDGELLAEDSIEFAGLDRKYQQSTFGAHFVEVGVDAATGETRVRRMLAVCAAGRI
                     LNPKTARSQVIGAMTMGVGAALSEALHVDPRLGCFVNHDLAGYEVPVHADIPHQEVIF
                     LDGADPVSSPMKAKGVGELGLCGVAAAIANAVYNATGVRVRDYPLTLDKLLGQMPAPG
                     "
     gene            complement(7407..8357)
                     /locus_tag="PK144_02385"
     CDS             complement(7407..8357)
                     /locus_tag="PK144_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNF64603.1"
                     /translation="MKPFTYERAGSPQAAVASAAAHPGARFIAGGTNLLDLMKLEIET
                     PAHLIDVNGLGLDQIEGTADGGLRIGALVRNSDLAADARVRRDYALLARALLSGASGQ
                     LRNRATTAGNLLQRTRCPYFYDSDLPCNKRLPGSGCAALGGYSRQHAILGVSEACIAA
                     HPSDMAVALRALDAVVETLRPDGSTRSLALADFHRLPGETPQVEHALEPGELIVAVTL
                     PPPPGGRHVYRKVRDRASYAFALVAVAVIVQPDGRGRVALGGVAPKPWRLEAAEAELP
                     RGASAVATALLAEARTTRENAYKTLLVERTLAAALAEARS"
     gene            complement(8354..9001)
                     /gene="paoA"
                     /locus_tag="PK144_02390"
     CDS             complement(8354..9001)
                     /gene="paoA"
                     /locus_tag="PK144_02390"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HNF64604.1"
                     /translation="MDDCLHLKITRRRLLIASAVSASAAALPSLSGAASRDAPQTAAV
                     PVQQRVRLRINGEVHSLELDTRTTLLDALREHLQLTGTKKGCDHGQCGACTVLLDGRR
                     INACLTLAVMHEGAEVTTIEGLGTPGRLHPMQAAFVKHDAFQCGYCTPGQICSALGML
                     DELRAGQPSHVSADLEAAPTLSADEIRERMSGNLCRCGAYSNLVEAITEAAGSRA"
     gene            9215..9562
                     /locus_tag="PK144_02395"
     CDS             9215..9562
                     /locus_tag="PK144_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64605.1"
                     /translation="MHESSIGPAPAVTGPAAPGAGGADAGGLQSKTGACATRNQPVIA
                     ALAGFRTVCPDKFNNQSNPADMMVFITVLTDKLSILLLWTKLMSASPASRHMRRKHAP
                     LPSFARAQDCAAG"
     gene            complement(9585..10484)
                     /locus_tag="PK144_02400"
     CDS             complement(9585..10484)
                     /locus_tag="PK144_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF64606.1"
                     /translation="MDRHATLLAFVSVVDAGGFAPAARRLGQSPAAVTRLVAALEARL
                     GVRLLARTTRAVTPTDAGQRYLERARALLAAFDEADEAVQDERSQARGHLVVAAPLLF
                     GRLHVAPLLAGFLADEPRVSAELRLGDHYAQLIEERIDVAVRIGPLADSTLVARPVGS
                     VRRVLVAAPDYLARHGHPQTPAALAAHELIACTALTSAAEWIFGDEARALRVPIAPRL
                     RSNSADAALLYAERGGGITALLSYQVAEAVRAGRLVSVLGSFAPPPLPVHLVYPSARG
                     LPARTRRFIDRVVTQADWQFADL"
     gene            10624..11184
                     /locus_tag="PK144_02405"
     CDS             10624..11184
                     /locus_tag="PK144_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF64607.1"
                     /translation="MNDTSRIPAFPSIEAAPAASRPLLDAVHAAFGAVPNLFRVAAHS
                     PAALEGLLGLHGALGKGAIDAATRERVALAIAEYDGCRYCLAAHAWLGARLAGLDADE
                     IAQNRRGRSHDLRADAALRFARRVAETRGHVGPGDLAAVREAGYGDGELVELVALVAL
                     NVLTNYLNEVAQTDIDFPVPAEPAAA"
     gene            11317..12693
                     /locus_tag="PK144_02410"
     CDS             11317..12693
                     /locus_tag="PK144_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004700783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HNF64608.1"
                     /translation="MPAAPFRPVAGRESRHVPLAGLLLAAVLAAGCGETATLPAEAGY
                     GPAPQLPEPKRTLIPTVNVAEASGWPQGVTPVPAPGLAVQAFADGLAHPRWLYVLPNG
                     DVLVAETDAPPKPEDGQGLKGWIMKWFMKRAGSSTPSANRITLLRDADGDGRPETRTV
                     FLEGLNSPFGMALVGEDFYVANTDALLRFPYRRGDTQIRAAPVKLADLPGGPLNHHWT
                     KNLIADRDGRTLYVTVGSNSNVAENGMAVEENRAAILAIERSSGKTRVYASGLRNPNG
                     LAWQPQTGQLWTVVNERDELGSDLVPDYLTSVQDGGFYGWPYSYYGAHVDARVEPPRP
                     ELVARALQPDYALGAHTASLGLSFYSGTLMPQYRNGAFIGQHGSWNREPPAGYRVVFV
                     PFRDGRPDGAVQEVLGGFRDGDQARGRPVGVAIDAHGALLVADDVGNTVWRVTPAVRP
                     AAAPVATR"
     gene            12860..15118
                     /gene="ppk1"
                     /locus_tag="PK144_02415"
     CDS             12860..15118
                     /gene="ppk1"
                     /locus_tag="PK144_02415"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNF64609.1"
                     /translation="MSVPPLDPLLPADAPAAVEARSANSPILTVAGPAETPAIPAVSN
                     AVPPDLDSPELYLNRELTWLAFNRRVLHEAEDARTPLLERVKFLAIVSSNLDEFFMKR
                     IGGLKQQIAAGVRTPSVDGRMPRQQLSECYAAVREINEQMRVLSGVLHGLLIAESIHV
                     RRCRNLTPEQQADLRQMYIRTVLPLVTPQAMDPAHPFPFISNLSLNLLVTVRYPHDEQ
                     VSLMRVKVPTGQGVERLIALENNTIFVPLEDVMAENLDLLVPGMVIEAVECFRVTRNA
                     VVEADEDQADDLLLLIESELRERRFAPTVRLEVNRGMDPVHRGMLAAELGLDEDTDVF
                     DVGGMMAMRDLFQIAGVDRPELHDTPHGPLDHPKLAGSPNIFHAIRDAGTILLQHPYE
                     SFATSVERFLREAARDPKVLAIKMTLYRTSADARIIQYLVEAARNGKQVAVVVELKAR
                     FDEAANIRWAERMEEAGIHVNYGVVGLKTHAKVIFVVRRDYSGLRRYAHIGTGNYHPG
                     TARLYTDLGLLTADEVIGDDLMELFNYLTTGFTPKRNYRKLLPAPKLLKPALLDRIER
                     EIRLHTTKNPGLIQFKMNALEDADIVRALYRAAQAGVRVDLIVRDTCRLRPGLPGISE
                     SVRVISIVGRFLEHPRLFYFRNGGHEEYFIGSADCMKRNLESRVEVVAPVEHPEARLE
                     LRAILDTQLNERRSAWDMQSDGSYVQRTPSENGETRGTHELLIAAAEARTREATRLKK
                     RRPKAIAKRSSL"
     gene            complement(15372..>15594)
                     /locus_tag="PK144_02420"
     CDS             complement(15372..>15594)
                     /locus_tag="PK144_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64610.1"
                     /translation="SMHLTRASIDLNRAYIDLNSSSTRVTRSSIDLNRACTRVTRTSI
                     DQEPHMDVRDAGHGRPYAAAGHPQLRRRG"
     assembly_gap    15595..15694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16161..16610
                     /gene="cynS"
                     /locus_tag="PK144_02425"
     CDS             16161..16610
                     /gene="cynS"
                     /locus_tag="PK144_02425"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNF64611.1"
                     /translation="MISDRSEVTKMILAAKVRKGIRWAEVAAKIGLSKEWTTAACLGQ
                     MTLDKTQAGIVGEIFELSDEAVAWLQIVPYKGSLPSAVPTDPLIYRWYEIVNVYGTTI
                     KELIHEEFGDGIMSAIDFSMDISREPDPKGDRVHVVLSGKFLPYKSY"
     gene            complement(16722..17987)
                     /locus_tag="PK144_02430"
     CDS             complement(16722..17987)
                     /locus_tag="PK144_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNF64612.1"
                     /translation="MPPESPAGLLRALFDAAVAAAQPALSLAAFLPEAPRGRTVVIGA
                     GKAATTMAQALDAAWPAALSGVVVTRHGQRQPCRRIEVLEAAHPVPDAHSVAAAARML
                     DAVRGLGADDLVICLISGGGSSLLVQPAAGLTLADKQAINRALLASGADIAAMNCVRK
                     HLSAIKGGRLAAACAPARVVTLAISDVPGDEPAVIASGPTVADATSCTDALAILDRYR
                     ITLPPAVRAALEAGELETPKPDDPCFARCEYHLIATPMRSLEAAAAVARAAGVTPLIL
                     GDALEGEAAEVAKVHAGIARSIAQHGRPLAAPCVLLSGGETTVTVRGRGRGGRNVEFL
                     LALAVALDGHPRVHALAADTDGVDGAEDIAGALCDPTTLARARALGIDPKASLADNDG
                     HGFFGALGDALVTGPTDTNVNDFRAILVR"
     gene            18328..18522
                     /locus_tag="PK144_02435"
     CDS             18328..18522
                     /locus_tag="PK144_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNF64613.1"
                     /translation="MTDTSTARSTRASVSFPPTLYEELVRIAAQKKVSVAWVIREAAE
                     RYVSERYPLFDGRPGSGEHE"
     gene            18519..19202
                     /locus_tag="PK144_02440"
     CDS             18519..19202
                     /locus_tag="PK144_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNF64614.1"
                     /translation="MSALPLSDADGDAAADLRRFVSGRSFGTILADPPWQFQNRTGKV
                     APEHRRLTRYGTLTLPEITALPVAEAAAEVCHLYLWVPNALLPEGLQVMAAWGFQYKS
                     NLVWHKIRKDGGPDGRGVGFYFRNVTELILFGVRGKNARTLAPGRSQVNFIASRKREH
                     SRKPDEQYELIEACSPGPFLELFARGERPGWQVWGNQADGEYYPSWDTYAHHSQVRDE
                     TAGVPVTDD"
     gene            complement(19223..19987)
                     /locus_tag="PK144_02445"
     CDS             complement(19223..19987)
                     /locus_tag="PK144_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNF64615.1"
                     /translation="MLDRLLESGFEIRCESHAAAILRIDFPEALEELESSLADIRIPI
                     TEIIGSGGGEAAGTQRLRRSLAGRGWTKTCFEIRKTLNGVERASISHEIDHVKTFANG
                     TLALEIEWNNKDPFFDRDLENFKRLHAEGGISAGIIVTRGSRLQQQLRSLVRRYAETR
                     NITDFEALAAVGVSLTPRQRTNISQRLQRSDDPLSAFAEAWAGQFVADKFGAATTHWD
                     KLMTRVQRGVGNPCPLLLIGIAETAVDFNDVAADDP"
     gene            complement(20159..21700)
                     /locus_tag="PK144_02450"
     CDS             complement(20159..21700)
                     /locus_tag="PK144_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNF64616.1"
                     /translation="MPPLTGAALAVAAVALSTATFMNVLDTTIANVSLPAIAGDLGVS
                     PTQGTWVITSFAVANAIAVPLTGWMTQRLGQLRLFLYSVLAFVCASLACGLAPTLESL
                     IFFRVVQGFVAGPMIPLSQALLLSCFPKEKAPMALGVWSMTTLVAPVAGPLLGGWLTD
                     NASWPWIFWINVPVGIVAAALTARVLKGRETPTRKLPIDGVGLGLLVLWVASLQLMLD
                     KGKELDWFSSPLILGLAITAAVGFVFFLVWELTEKHPIVDLSLFRSRNFTTGVLAIAL
                     AYGVFFGNVVLMPLWLQQYMGYTATWAGIVTAPTGVLAVLLTPLVARLMPRTDPRLLA
                     SFAFVVFAVVAFMRSGFTTGADAVTLAMPQLVMGAAMSTFFVPLTAMLLSGLPPERIP
                     AASGLSNFARITAGAIGASVYTTQWEDRAALHHAQLVEHLTPYSLTTTQTLNTYAQQF
                     GLDPQQALGALDRQVTQQSVLLAADDLFWLSGMIFLVLIGVVWLARPKKQGGAAGGAA
                     AAGAH"
BASE COUNT         3396 a         7458 c         7576 g         3203 t
ORIGIN      
        1 tcggtcgagt tcacgccgca cgccttcctg aaggcgatcg gctggggcac gagtgcgaag
       61 gaatacacgc ggctgcgcga gcacctgtcg cgcatgcagg cgaccgcgct gtcggtctac
      121 agccggcgcc tcggcaaggg ctgcagcgtg tcgctgatcc gcaagttcga gttcgccgac
      181 gaggccggcc agaagctcgt gcgctggcgc gtctacatcg agccggagat gcacatcctg
      241 ttcggcggcg tctactacac gcagctcgaa tgggaacagc gcatgcgcct cggcacgctg
      301 gcctgctggc tgcacggcct gtattcgagc cacacgcagc cgttcccgat gaaggtcgag
      361 acgctgaagg acggctgcgg ctcggcggcg cgggatctca agaacttccg ctaccggctg
      421 cgcaggtcgc tcgaagagct gcgccaggtc ggcttcctga gccactggta catcgaccgc
      481 accgatctcg tccacgtcca gcgcaccgcc gtaccgaccg acgaggccct gcccgccgcc
      541 accgcggaca gcgatcccga caccgcaacc gaaccaccaa tcccgcccgg cgccgacccg
      601 gacgcgccgg acggcaactc aaaacccgaa tgaagccgaa gccacgcgat ccggccggaa
      661 gtcatccgct gtgggcggtc tgtggatgaa tctgtggatg gattgtgagt aaacccgcaa
      721 ccagttcggg ttttgagttg ccgaaacggg ctctcgttcg ggttttgagt tgccatgatt
      781 cgggttttga gttgccgaag ttcgggtttt gagtttcaaa agttcgggtt ttgagttgct
      841 gtggatatct acaagcaatt gtactgaaag ggaaaaaagg catttttttt tgatgctaac
      901 cttctttaac ctatttataa ccaaaaaaac gcgcgcgcgc atgcacgtat acgcgcatgc
      961 acgcgtacgc gcgaggacgt tcatcgaaaa aatggtcttc agaaagaacg gcaccggtct
     1021 gcgtccaccg atcgacctcc gcaggacgat tcgcgccgga cgaggcatgc atccggtgct
     1081 gctgaacaac ttcgcactgc atcaggcaac ggcaccgaca acgacgtcgg aaactcaaaa
     1141 cccgaacgac agcagcaaaa cgtccgcacg acacggccac tgccagtcct catggccaga
     1201 acgtacgtcc tgcggatacg agcctgtcga tcatcgacag gccacagctt cggcaactca
     1261 aaacccgaac gctgtccgca ttgccaccgg cgtgccggcg gcaatcagat ggtccggtcg
     1321 aaagcccgca gctccgggcc tgccagccgg tagagctgcg ccgggcggct ggagcggtcc
     1381 tgcgagaagg cccccggcac cggcacgatg aagccctgcg cctcgatctt gcgccggaac
     1441 gaggccgggt ccgggcggct gccgagcacg cggcagtaga ccgcgtagag ctgcggcagc
     1501 gtgaagctct catcgagcag gaaggccggc agcgacgagt acgtcgcctt gtcacgcacc
     1561 cgtgccagcg cccgcgccac gatgtcggca tgatcgaagg cgagcaccgg cagcgtcgcg
     1621 accggctgcc agcgcgcgcc ggccggcagc gccgcaatcc gcccggccgg cagcatcgcg
     1681 aagtaggcga tgctgatcga ccagccgcgc ggatcgcgcg tgcggctgcc gaagctgtag
     1741 agctgttcga gatacggcgc ctgcacgccg agcttgccgg cgagcacgcg ccgcgcggtg
     1801 ccggcggtat cggcatcctc atcgacgtgc acgtagccgc cgggcagcgc cggccggccg
     1861 cggaacggct cctgcgcacg ctcggcgatc agcacctgca ggccgtcgtc atcggcgggt
     1921 tctgccagcg tgaacagcac gatgtcgacg gtggcgatcg ggcggaggtg ttcgctcatc
     1981 tcggggcttc cggcgcgtcg tggcggctgc aggcaggggg cggcggcgcg cagcctaaaa
     2041 ggtcattcct atttcttgca aatggaaact tgcaatttgc atttatgtgg atcagtcttg
     2101 gtgccgtcac caacgaccgc cacgagagga gcgccgccat gggttacgcc cgctgggatt
     2161 ccgatgactg gagcgattac gccgccggcc aggccggccg cagccgcgag gaagtgttca
     2221 cgcgccgcag cctcgatccc gagttcgatc cgctgaacat ccgccggcgc gagtcgcgcg
     2281 attcggcgca gaaccccgaa tcgaacgcga tcatcgtcgc cttcgacgaa accggctcga
     2341 tgggcagcat tcccgatgcc ttcgtgcgcg agggcctcgg cacgatggtg caggcgctgc
     2401 tcgaccgccg gccggtcagc gatccgcacg tgatggtcat gggtttcggc gacgcctggt
     2461 gcgaccgcgc gccgctgcag gtcacgcagt tcgaggccga cctgcgcatc gccgatcagc
     2521 tctcgcggct gtggctcgaa ggcggcggcg gcggcaaccg cttcgagagc tacaacctgc
     2581 cgtggtactt cgcggcgcgg cgcacgtcga tcgactgctt cgagaagcgc ggcaggaagg
     2641 gctatctgtt cacggtcggc gacgagccgc cgccgccgaa gctgctcgcc gcgcacgtgc
     2701 gcgccttcct cggcgatgac ctgcgccgcg acctcgacaa cgccgagctg ctgcgcgccg
     2761 ccgggcgcat gtacgaaatc ttccacgtcg tcgtcgagca gggcagccac taccgcagct
     2821 tcccggacga ggtcgaacag ggctggacgc ggctgctcgg ccagcgcgtg ctgttcctcg
     2881 ccgactaccg ccagctcgcc gaagtcatcg tctcggcgat cgaagtcaac gagggccgcg
     2941 acccggatgc cgtggtcgcg agctgggacg ccgccgccgg cacagtcgcg aaggccttgc
     3001 gccggctgcc ggcagcgtcg cgccggcgcg gcctgctcgg ctacttcggc cggagcgccc
     3061 gctgatgcgc gcgctcgcgg tgatcggcgc cggctacggc gatgaaggca agggcgcggc
     3121 caccgaccgg ctggccgcga ccctcgggcc gcgggcgctg gtcgtgcgcc acaacggcgg
     3181 cgcgcaggcc ggccacaccg tcatcgcgcc cgatggccgc cggcacgtgt tcggccacat
     3241 cggcagcggc agtttcgccg gcgtgccgag ctacctgtcg gagtacttcg tcgccagccc
     3301 gatcctgtgc ctgcgcgaac tgagggcgct tgccgtactc ggcgtgcagc cgaagctgca
     3361 cgtcgatgcc ggctgcgcgg tgacgacgcc gtacgacatg ctgctgaacc aggccgtcga
     3421 ggccgcacgc ggagccggcc ggcacggctc ctgcggcgtc ggcttcggcg agacgatcga
     3481 acgccacggc cacgccggtt tcgcgctcac ggtcgccgac ctgcgcaaac ccgtgcggct
     3541 gcgcgaacgg ctcgaagcga tccgccgcga ctggctgccc gcgcgcgccg cggcgctcgg
     3601 tctcgaccgg ctgccagcgg tggcttacga caatgcgctg tcggtgcgct tcgtcgacga
     3661 ctgcgctcgc ttcctggccg caatcgaggt ctgcgatgcc ggcgtgctgc gcggccgacc
     3721 gctgatcttc gagggcgcgc aggggctcgg cctcgacatg gaccgcggcg ccttcccgca
     3781 cgtcacgcgc tcgcacaccg gcatccgcaa cgccgtgacg ctgactcgcg cggccggcct
     3841 cgacgcgctg caggtgcagt acgccacccg cgcctacgcc acccgccacg gcgccggccc
     3901 gctcgcgcac gaactgccgg ccgcaccggc cgcggcggtg cgcgatgcga ccaaccgccc
     3961 gaacgactgg cagggccggc tgcgcttcgg ctggctcgac ctcgaccgcc tcgccaccga
     4021 catccggacc gacctcgccg atgccggcgc gctggcggtg acggcggagc tgatcgtcag
     4081 ctgcctcgac cagatggacg aggtgatccc gcacgtcgcg gacggcggac tcgtgcaggt
     4141 gccgcgcgaa cgctgcctgg cgtggtacgc gcagcggctg ggactgaggg ccggttacgg
     4201 cgcgtacgga ccggggcggg atgcggtgcg ggaatgggcg cgggcggcgt gaggccattg
     4261 cagcgcgact gccggcgcag gccgtcccgg gcagcgcggt cattcgcgaa acggaccgcg
     4321 cgccgggttc cctcctcaac gccggcgatc agggcatgca ggcagccgcg gggcgcaggg
     4381 cccatcaggc acgattcttc tgcgcctcga cgtatttcct gagcacgcgg ttgatgagtg
     4441 tctgatagcc cctgccctgt tcccggaaat aaccgaggac atcggcatcg attctcagcg
     4501 tgatctgctt cttcggtccc ggcaagatca cggcctgcgc cagaaaatgt gcgtcgcgtt
     4561 ccggatcgtc ggagaagtcg atgtcatcgt ccgtcatcgc ctgcagacgg gcccagtcgg
     4621 tcgcggatgg cggtttcgta tgcgatgcgc tcattcttgc tggccttcct cgcagaaatg
     4681 attctgacga tgcggggttc gcgttccgtg aacacgaagg cgatcgtgat gccctgaatc
     4741 ctgccgatgg cctgccagcg gatttctcca tagtccctgc gggtgtcccg ggcaatgagg
     4801 atggggttgg ccgtcacccg cggcaggtcc tggaaatcaa tgccgtgctt ttcgatgttg
     4861 ctggcgttct tctgctcatc ccacgcccat tcgacgtgat cgtcgaaacc atgctcctga
     4921 tagcgatcgg acatcgcagg ctccgggcgc cggcaggatg tgccttcgcc tgatcgatcg
     4981 ggcgacagaa gcttgaatcc gtggatcacc gcatggtgta actacaaacg tagttacact
     5041 acgcgcccac gtcaacaggg cggatgcgca cgtggcgcac ggcgtccctg aagccggcgg
     5101 acagcttgca ggagggcgga aagcggcaag tcgcggtggc ggctgcgatg gccgcagcac
     5161 cgggccgctg ccgcgtggat cagcccggtg ccggcatctg cccgagcagc ttgtcgagcg
     5221 tcagcgggta gtcgcgcacg cgcacgccgg tggcgttgta gacggcgttg gcgatggcgg
     5281 cggcgacgcc gcacaggccg agttcgccga cgcccttggc cttcatcggc gaggacaccg
     5341 gatcggctcc gtcgaggaag atgacctcct ggtgcgggat gtcggcgtgc accggcacct
     5401 cgtagccggc aaggtcgtgg ttcacgaagc agccgagccg cgggtcgaca tgcaaggcct
     5461 cgctgagcgc ggcgccgacg cccatcgtca tcgcgccgat cacctggctg cgtgccgtct
     5521 tcgggttcag gatgcgcccg gccgcgcata cggccagcat ccggcgcacg cgcgtctcgc
     5581 cggtcgcggc atcgacgccg acctcgacga agtgcgcccc gaaggtcgac tgctggtact
     5641 tgcggtcgag accggcgaac tcgatcgaat cctcggcgag cagttcgccg tcgcgcacgg
     5701 catcggcgag gggcgcgacg cgcccgccgg cctgcacctc gccgtccgcg aagcgcacgg
     5761 ccgcggcatc cctgaagccg agccgctgcg cgatctgctc gcgcagacgc atgcaggcgg
     5821 cgtagacgcc ggcggtcgag cagttgccgc cccactgccc gccggagccg gccgagaccg
     5881 ggaagtccga atccccgagg cgtacgtcga cctgcgcgag cggcacgccg agcagctcgg
     5941 cggcagtctg ggcgatgacg gtgtagctgc cggtgccgat gtcggtcatg tcggtctcga
     6001 ccgtcacgcg tgcgtcgcgc ccgagccgca cgcgcgcgcc ggatttcatg accaggttgt
     6061 tgcgaaacgc cgcggcgacg ccgtggccga gcagccagcg gccggcgcgg gtgcggccgg
     6121 ggacgggatt gcgctgcgcc cagccgaagc gcgcggcacc ggtgcgcagg cactcgacca
     6181 gctggcgctg cgagaagcgc cgctgcgggt cgtgcggatc gaccggcgtg tcgttgcgga
     6241 tgcgcagctc gaccggatcg aggccgagct tttcggcaag ctcgtcgatc gcgatctcca
     6301 gcgccatcag tcccggtgcc tcgcccgggg cgcgcatcgc gttgccttcg ggcaggtcga
     6361 gcgtcgccag gcgcagcgcg ctcaggcgat gggcgccggc atagagcagc tgcgtctgct
     6421 gggtcgccgt ctcggcgctg ccgcccggca ggttgccgca ccagctctcg tgggcgatcg
     6481 cgtggatgtg gccgtctgcg gtcgcgccga tgcggatgcg ctggatcgtc gccggccggt
     6541 gcgtggtgtt gttgaagacc agcggccgcg caagcatgac cttgaccggc cggccgatcg
     6601 cacgcgcccc gagcgcagcg agcacggcat cggcgcgcac gaacagcttg ccgccgaaac
     6661 cgccgccgat gtacggcgac agcagccgga tctgctccgg cctgatgccg agcgtcttcg
     6721 cgagatcggc gcggccccag gcgatcatct ggttcgaggt ccagagcgtc agccgatcgc
     6781 cgtcccaggc cgccatcgat gcgtgcggct ccatcatcgc gtgcgactgg tcgggcgtgc
     6841 ggtagacggc gtcgatgcgc acggccgagg cggcgaaggc cgcggcgaag tcgccgagcc
     6901 ggcggtcggg ttcgtcctgc ggcgcccggc cgctgtcgcg cacctttgcc agatcgaact
     6961 cgccggcctc gcgcgcgtat tcgacgcgga tcagccgcgc cgcggcgcgc gcctgctcga
     7021 agctttcggc gacgaccagc gcgatggcct ggtgatagtg ctcgatccgc ggcccgccga
     7081 gcagcttcgc ggtgttgcgc ggaccctggc cgagcgggcc ggcgttgtcg gcgctgatca
     7141 ccgcgagcac gccgggcgcg cggcgcgctg cttcggcatc gaacgagcgg atgcggcccc
     7201 tggcgatgcc ggcaccgagc acatgaccgt acgcgggcct ggcgagaccg tctgcgaagc
     7261 cgcccgtgaa gccgtcgtga tgctcgtagg catacggcgc acggccggtg gtcttcagcg
     7321 ggccgtcgat gcggtcgacg ggctggccga cgacacgcag gcgatcagtg gggttttcag
     7381 cggatggcgt gtcgaatttc atcggctcag ctccgggctt cggccagcgc cgccgcgagc
     7441 gtgcgctcga ccagcagcgt cttgtaggcg ttctcgcgcg tggtgcgtgc ctcggcgagc
     7501 agcgcggtgg cgacggcgga cgcaccgcgc ggcagttcgg cctcggctgc ttcgaggcgc
     7561 cacggcttcg gcgcgacgcc gccgagcgcg acgcgcccgc ggccgtcggg ctggacgatg
     7621 acggcgaccg cgaccagcgc gaacgcgtac gaggcgcggt cgcggacctt gcggtacacg
     7681 tgccggccac cgggcggcgg cggcagcgtc acggcgacga tcaactcgcc gggctccagc
     7741 gcgtgctcga cctgcggcgt ctcgccgggc aggcgatgga agtcggccag cgcgaggctg
     7801 cgcgtgctgc cgtccgggcg cagcgtctcg accacggcgt cgagcgcgcg cagcgccacg
     7861 gccatgtcgc tcggatgcgc ggcgatgcag gcctcgctga cgccgaggat cgcgtgctgg
     7921 cggctgtagc cgccgagtgc cgcgcagccg ctgccgggca ggcgtttgtt gcacggcagg
     7981 tcgctgtcgt agaagtacgg gcagcgcgtg cgctgcagca ggttgccggc ggtggtcgca
     8041 cggttgcgca gctgccccga ggcgccggag agcagcgcgc gcgcaagcag cgcgtagtcg
     8101 cggcgcacgc gcgcatcggc ggcgagatcg ctgttgcgca ccagcgcacc gatgcgcagg
     8161 ccaccgtcgg cggtgccctc gatctgatcg agaccgaggc cgttcacgtc gatcaggtgc
     8221 gccggcgtct cgatctccag cttcatcaga tcgagcaggt tggtgccgcc ggcgatgaag
     8281 cgcgcgcccg gatgcgcggc ggcactggcg accgctgcct gcggcgagcc ggcgcgttca
     8341 tacgtgaacg gtttcatgcg cggctcccgg cggcttcggt gatcgcttcg acgaggttcg
     8401 agtaggcgcc gcagcggcag aggttgccgc tcatgcgctc gcggatttcg tcggcgctca
     8461 gtgtcggtgc ggcttcgaga tcggcgctga cgtggctcgg ctggccggca cgcagttcat
     8521 cgagcatgcc gagggccgag cagatctggc ccggcgtgca gtaaccgcac tggaaggcat
     8581 cgtgcttcac gaaggccgcc tgcatcggat gcagccgtcc gggcgtgccg aggccctcga
     8641 tcgtcgtgac ctcggcgccc tcgtgcatga cggcgagcgt caggcaggcg ttgatgcgcc
     8701 ggccgtcgag cagcaccgtg caggccccgc actggccgtg atcgcagccc ttcttggtgc
     8761 cggtcagctg cagatgctcg cgcagcgcat cgagcagcgt cgtgcgcgta tcgagttcga
     8821 ggctgtgcac ctcgccgttg atgcgcagcc tcacgcgctg ctgcaccggg actgccgcgg
     8881 tttgcggcgc atcgcggctt gcggcgcccg acagcgacgg cagcgccgcc gcactggccg
     8941 acacggcgct cgcgatcagc agccggcggc gggtgatctt cagatgcagg cagtcgtcca
     9001 cgggttgttt tcctcgacgc gggcgggaag acggaccggt cggggtaccg caggcagcga
     9061 ggcagcctga cagccggccg gcagcggcgg acttcggctc cggacgggcc ggaacggaaa
     9121 cggcagaagc cggcggcccg gcgcaggccg gccgtcagcc cgggtttcga gccacgcccg
     9181 cgcgccgcgt gccggccgcg cacgacctgc tgctatgcac gaatcgtcca tcggcccggc
     9241 gccggccgtg acaggtccgg ctgcgccggg cgccggcggg gctgatgccg ggggtctgca
     9301 gtcgaaaaca ggtgcctgcg caacccgtaa tcagcccgta atcgccgctc tggccggttt
     9361 tcgcactgtt tgccctgata agttcaataa tcaatcaaat cctgcagata tgatggtttt
     9421 tataactgta ttaaccgata aattatcaat tctgctgctc tggacgaagc tcatgtcggc
     9481 cagccctgca agtcgtcaca tgcgcaggaa gcatgccccg ctgccttcct tcgcgcgtgc
     9541 gcaggactgc gctgccggat gaacgcacgg gcggcgcgca gggctcagag atccgcgaac
     9601 tgccagtcgg cctgcgtgac gacgcgatcg atgaagcgcc gggtgcgggc cggcaggccg
     9661 cgggcgctcg ggtagaccag atgcaccggc agcggcggcg gggcgaagct gccgagcacg
     9721 gacacgagcc ggccggcgcg cacggcttcg gcgacctgat acgacagcag cgcggtgatg
     9781 ccgccgccgc gctcggcgta caggagggct gcatcggcgc tgttgctgcg caggcgcggg
     9841 gcgatcggca cgcgcagcgc ccgggcctcg tcgccgaaga tccactccgc ggcactcgtc
     9901 agcgccgtgc aggcgatcag ttcgtgtgcg gccagcgcgg ccggggtctg cgggtggccg
     9961 tgccgcgcca gataatcggg ggcggcgacc agcacgcggc gcacgctgcc gaccgggcgt
    10021 gcaaccagcg tcgaatcggc gagcgggccg atgcgcacgg ccacgtcgat gcgctcctcg
    10081 atcagctgcg cgtagtgatc gccgaggcgc agctcggcgc tgacccgcgg ctcatcggcg
    10141 aggaagccag ccagcagcgg cgcgacgtgc agccgcccga acagcagcgg agcggcgacc
    10201 acgagatgtc cgcgcgcctg gctgcgttca tcctgcacgg cttcgtcggc ctcgtcgaag
    10261 gccgcgagca gcgcgcgtgc ccgctccaga tagcgctggc cggcgtcggt cggcgtcacc
    10321 gcccgcgtcg tgcgcgccag caggcgcacg ccgagccggg cttcgagcgc cgcgaccagg
    10381 cgcgtgactg cggccggcga ctgcccgagc cggcgcgcgg ccggggcgaa gccgccggcg
    10441 tcgacgacgc tgacgaaggc aagcagcgtg gcatggcggt ccatggattc ctccgtctga
    10501 cgcaatgatg tcgtgcagag caaacggatt atcgtcaccg gcatcggcaa tcacactggc
    10561 tcggcatcgg cggcgctccc ggccgccgaa cctgccgaga gccatgccgc catgaacgac
    10621 acgatgaacg acacgtcccg catccccgcc ttcccgtcga tcgaggccgc tccggccgcg
    10681 tcccggccgc tgctcgatgc cgtgcacgct gccttcggtg ccgtgcccaa cctgtttcgc
    10741 gtcgccgcgc acagcccggc ggcgctcgaa ggcctgctcg gcctgcacgg cgcgctcggc
    10801 aagggcgcga tcgacgccgc gacccgcgag cgcgtggcac tcgccatcgc cgaatacgat
    10861 ggctgccgct actgcctggc cgcgcacgcc tggctcggtg cccggctcgc cgggctcgat
    10921 gccgacgaga tcgcgcagaa ccgccgcggc cgctcgcacg atctgcgcgc cgatgcggcg
    10981 ctgcgcttcg cgcgtcgcgt cgccgagacc cgcggccacg tcggccccgg cgatctggcc
    11041 gcggtgcgcg aggccggcta cggcgatggc gagctggtcg aactcgtggc gctggtcgcg
    11101 ctgaacgtgc tgaccaacta cctgaacgag gtcgcgcaga ccgacatcga cttcccggtg
    11161 ccggcggaac cggcggcggc ctgaaagccg gcaggacccg gcgctgcgct gcgcgccggt
    11221 cccgcgccgg catgagcaaa cgttcatgcc ccggaacggg gccggcggcc ggccggtcgc
    11281 acagcggcaa tccccccgtg ccgccggagt ccgccgatgc ctgccgcccc gtttcgcccc
    11341 gtcgccggcc gcgagtcgcg gcatgtgccg ctcgccggcc tgctgctggc tgccgtcctc
    11401 gccgccggct gcggcgagac cgcgacgctg ccggccgagg ccggctacgg accggcaccg
    11461 cagctcccag agccgaagcg cacgctgatc ccgaccgtca acgtcgccga ggccagcggc
    11521 tggccgcagg gcgtgacgcc ggtgccggcg ccggggctcg ccgtgcaggc cttcgccgac
    11581 gggctcgcgc atccgcgctg gctgtacgtg ctgccgaacg gcgacgtgct ggtcgccgag
    11641 accgacgccc cgccgaagcc cgaggacggc caggggctga agggctggat catgaaatgg
    11701 ttcatgaagc gcgccggcag cagcacgccg agcgccaacc gcatcacgct gctgcgcgat
    11761 gccgacggcg acggccggcc ggaaacgcgc acggtgttcc tggagggact gaactcgccg
    11821 ttcggcatgg cgctggtcgg cgaggatttc tatgtcgcca acaccgatgc gctgctgaga
    11881 ttcccgtacc ggcgcggcga cacgcagatc cgcgccgcac cggtgaagct cgccgacctg
    11941 ccgggcgggc cgctgaacca tcactggacc aagaacctca tcgccgaccg ggacggccgc
    12001 acgctgtacg tgacggtcgg ctccaacagc aacgtcgccg agaacggcat ggcggtcgag
    12061 gagaaccgcg cggcgatcct cgcgatcgag cgcagcagcg gcaaaacccg cgtctacgcc
    12121 tcgggcctgc gcaaccccaa cggcctggcc tggcagccgc agaccgggca gctgtggacc
    12181 gtcgtcaacg agcgcgacga actcggcagc gatctggtgc cggattacct gacctcggtg
    12241 caggacggcg gcttctacgg ctggccgtac agttactacg gcgcgcacgt cgatgcccgc
    12301 gtcgagccgc cgcgcccgga gctggtcgca cgggcgctgc agcccgatta cgcgctcggt
    12361 gcgcacaccg cctcgctcgg cctgagcttc tattcgggca cgctgatgcc gcagtaccgc
    12421 aacggcgcct tcatcggcca gcacggttcg tggaaccgcg agccgccggc cggttatcgc
    12481 gtcgtgttcg tgccgtttcg cgacggccgg cccgacggcg ccgtgcagga ggtgctcggc
    12541 ggctttcgcg acggcgatca ggcgcgcggc cggccggtcg gggtcgcgat cgacgcgcac
    12601 ggggcgctgc tggtcgccga tgatgtcggc aacaccgtct ggcgcgtgac gccggcggtc
    12661 aggccggcgg cagccccggt ggcgacgcgc tgaagccgcg cgacatccgt aatcaaaccg
    12721 tcatgcgact gtcgtcaaag cgtcgcaaaa tgggcagacg ccgtctgtcg gtgcgtgcgc
    12781 gtgcggttgc cggcttcgag tccggatgca ccggtcaccg gcggcggcgg ccgtcgcgag
    12841 ccaccggagt tcccgcacga tgtccgttcc accactcgac ccgctgctgc cggccgacgc
    12901 gccggcagcc gtcgaggcgc gcagcgccaa ttccccgatc ctcacggttg ccggtccggc
    12961 cgaaaccccg gccattcccg ccgtcagcaa tgccgtaccg cccgatctcg acagccccga
    13021 gctgtacctg aaccgcgagc tgacctggct ggcgttcaac cgccgcgtgc tgcacgaggc
    13081 cgaggacgcg cgcacgccgc tgctcgaacg cgtgaagttc ctcgcgatcg tcagctccaa
    13141 cctcgatgaa ttcttcatga agcgcatcgg cggcctgaag cagcagatcg ccgccggcgt
    13201 gcgcacgccg agcgtcgacg ggcgcatgcc gcgccagcag ctcagcgagt gctacgcagc
    13261 cgtgcgcgag atcaacgagc agatgcgcgt gctctccggc gtgctgcacg gactgctgat
    13321 cgccgagagc atccacgtgc ggcgctgtcg caacctcacg cccgagcagc aggcggatct
    13381 gcgccagatg tacatccgca cggtgctgcc gctggtgacg ccgcaggcga tggacccggc
    13441 gcatccgttc ccgttcatct ccaacctgtc gctgaacctg ctggtgacgg tgcgctaccc
    13501 gcacgacgag caggtgtcgc tgatgcgcgt gaaggtgccg accggccagg gcgtcgagcg
    13561 gctgatcgcc ctggagaaca acacgatctt cgtgccgctc gaggacgtga tggccgagaa
    13621 cctcgacctg ctggtgccgg gcatggtcat cgaggcggtc gagtgcttcc gcgtcacgcg
    13681 caacgccgtg gtcgaggccg acgaggatca ggccgacgac ctgctgctgc tgatcgaatc
    13741 cgagctgcgc gagcggcgct tcgcgccgac cgtgcgcctg gaggtcaacc gcggcatgga
    13801 cccggtgcac cgcggcatgc tcgccgccga actcggtctc gacgaggaca ccgacgtgtt
    13861 cgatgtcggc ggcatgatgg cgatgcgcga cctgttccag atcgccggcg tcgaccgccc
    13921 cgaactgcac gacacgccgc acggtccgct cgaccaccct aagctcgccg gctcgccgaa
    13981 catcttccac gccatccgcg acgccggcac catcctgctg caacacccct acgagtcgtt
    14041 cgcgacctcg gtcgaacgct tcctgcgcga ggcggcgcgc gatccgaagg tgctggcgat
    14101 caagatgacg ctgtaccgca cctcggccga tgcgcggatc atccagtacc tggtcgaggc
    14161 cgcgcgcaac ggcaagcagg tggcggtggt cgtcgagctg aaggcgcgct tcgacgaggc
    14221 cgccaacatc cgctgggcgg agcgcatgga agaggccggc atccacgtca actacggcgt
    14281 cgtcggcctc aagacccacg ccaaggtcat cttcgtcgtg cgccgcgact actcgggcct
    14341 gcgccgctac gcgcacatcg gcactggcaa ctaccacccg ggcacggcgc ggctgtacac
    14401 cgacctcggc ctgctgaccg ccgacgaggt gatcggcgat gacctgatgg agctgttcaa
    14461 ctacctgacc accggcttca cgcccaagcg caactaccgc aagctcctgc cggcgccgaa
    14521 gctgctgaag cccgcgctgc tcgatcgcat cgagcgcgag atccgcctgc acaccacgaa
    14581 gaaccccggc ctgatccagt tcaagatgaa cgcgctcgag gacgccgaca tcgtgcgtgc
    14641 gctgtaccgc gccgcgcagg ccggcgtgcg cgtcgacctg atcgtgcgcg acacctgccg
    14701 gctgcgtccg ggcctgccgg ggatttcgga gagcgtgcgc gtgatcagca tcgtcgggcg
    14761 cttcctcgaa cacccgcggc tgttctactt ccgcaacggc ggccatgagg aatacttcat
    14821 cggctcggcc gactgcatga agcgcaacct cgaaagccgc gtcgaggtcg tcgcgccggt
    14881 cgagcacccg gaggcgcggc tggagctgcg cgcgatcctc gacacccagc tcaacgagcg
    14941 gcgcagcgcc tgggacatgc agtccgacgg ttcctacgtg cagcgcacgc cgtccgagaa
    15001 cggcgagacg cgcggcacgc acgagctgct gatcgccgcc gccgaggcac gcacgcgcga
    15061 agccacgcgt ctgaagaagc gccggccgaa ggcgatcgcc aagcgcagca gcctgtaatc
    15121 cggcatcggc acacggtttc gcccctgtct ccgccccgct ttccgcaagg gaagcggggc
    15181 ggcggcgttt gtgcgcggct ggcgtcctga tccggtggcg acgccgggga gggtcgcggg
    15241 gggcacagac cgggccgaag gcagcgcagc cgtcatttcg cgtgtttctg ggtgattgca
    15301 gggctgttcc ggccggttgt tccgggaatc ctgcaatgtg agcgtggttt cctgcgcttg
    15361 ccatgcacgc atcagcccct cctgcgaagc tgtggatgcc cggccgcagc gtatggccgg
    15421 ccgtgaccgg catcacgcac gtccatgtgc ggttcctgat cgatggaagt acgggtcacg
    15481 cgtgtgcaag cgcggttcag gtcgatcgaa ctgcgggtca cacgcgtgga actgctgttc
    15541 aggtcgatat aagcacggtt cagatcgatg gatgcacggg tcaagtgcat ggaannnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgcagt ccagatcgat ggatgcacgg
    15721 gtcaagtgca tggaactgca gtccagatcg atgggcgcac gggtcaggtg catggaactg
    15781 cagtccagat caatggatgc acgggtcagg tgcatggaac tgcagtccag gacgatcgaa
    15841 gcatgggtca cacgggcgca agtgcggttc aagtcgatta aaccgcggct cacacgcccg
    15901 caagtgcgca tcgccgtgtc cggtgccgga ctgcgccggc tgccgccggc gcctcgaccg
    15961 gcgcctgccg atcgccgtgc aaggctgcgc cttgcggccg cctgccgcgc gtgggtcagg
    16021 cgtcatggga agaaaaaacc gcgcatctgc gtgtgatcgc cggcggccgt gccagtcttc
    16081 gccgggtgcc cgccgcaatg cagcacggcg gcgggccgtg cctggccatc cccgtcctgt
    16141 caccccggag tccgtcgccg atgatcagcg atcgcagtga agtcacgaag atgatcctgg
    16201 ccgccaaggt ccgcaaaggc atccgctggg ccgaagtggc cgcaaaaatc ggcctcagca
    16261 aggaatggac gacggccgcc tgcctcggcc agatgacgct cgacaagacc caggccggca
    16321 tcgtcggcga gatcttcgaa ctgtccgacg aggcggtcgc gtggctgcag atcgtgccgt
    16381 acaagggctc gctgccgagc gcggtgccga ccgatccgct gatctatcgc tggtacgaga
    16441 tcgtcaacgt ctacggcacg accatcaagg aactgatcca cgaggaattc ggcgacggca
    16501 tcatgagtgc gatcgacttc tcgatggaca tctcgcgcga acccgacccc aagggcgacc
    16561 gcgtgcacgt cgtgctgtcg ggcaagttcc tgccgtacaa gagctactga gcccgatccc
    16621 ggcgcaacag ccggtccttt ccgccccttc cccctggcag gggaggaggg gagagggcgg
    16681 ccgaccacgc attcgtgcgg gatcggcatg cggcccgtgg ctcagcgcac caggatcgcg
    16741 cggaagtcgt tgacgttggt gtcggtcggg ccggtgacga gcgcatcgcc gagcgcgccg
    16801 aagaagccgt ggccgtcgtt gtcggcgagg ctcgccttcg ggtcgatgcc gagcgcgcgg
    16861 gcgcgggcga gggtggtcgg gtcgcacagc gcaccggcga tgtcctcggc accgtcgacg
    16921 ccgtcggtgt cggcggcgag cgcgtgcacg cgcggatggc cgtcgagcgc gacggcgagc
    16981 gcgagcagga actcgacgtt gcgcccgccg cgcccgcgtc cgcgcacggt gaccgtggtc
    17041 tcgccgccgg agagcagcac gcagggcgcg gcgagcggcc ggccgtgctg cgcgatcgag
    17101 cgggcaatgc cggcgtgcac ctttgcgacc tcggcggcct cgccttcgag cgcatcgccg
    17161 aggatcagcg gcgtgacgcc ggcagcgcgc gccaccgccg cggcggcttc gagcgagcgc
    17221 atcggcgtcg cgatcaggtg gtactcgcag cgcgcgaagc agggatcgtc cggcttcggc
    17281 gtttccagct cgccggcttc gagcgccgcg cgcacggccg gcggcagggt gatgcggtag
    17341 cgatcgagga tcgccagcgc atcggtgcag ctcgtcgcat ccgccacggt cggcccggag
    17401 gcgatcaccg ccggctcgtc gccgggcacg tcggagatcg ccagcgtcac gacccgcgcc
    17461 ggcgcgcagg ccgcggccag ccgcccgccc ttgatcgccg aaagatgctt gcgcacgcag
    17521 ttcatcgccg cgatgtcggc accgctcgcg agcagcgcgc ggttgatcgc ctgcttgtcg
    17581 gcgagcgtca gccccgcggc cggctgcacc agcagcgacg agccgccgcc ggagatcagg
    17641 cagatcacca gatcgtcggc gccgagcccg cgcaccgcat cgagcatgcg cgcagccgcc
    17701 gcgacgctgt gcgcatccgg caccgggtgc gcggcttcga ggacctcgat gcgccggcac
    17761 ggctggcgct ggccgtggcg cgtgacgacg acgccggaca gcgctgcggg ccacgcggca
    17821 tcgagcgcct gcgccatcgt cgtcgccgcc ttgccggcac cgatgacgac ggtacgcccg
    17881 cgcggcgctt cgggcaggaa ggcggcgaga ctcagcgccg gctgggcggc ggcgaccgcg
    17941 gcatcgaaca gcgcgcgcag cagtccggcg ggggattcgg ggggcatgga ggcggtctcc
    18001 gcaggcatct ggaaacggat cggcgctgcg taccaaccat ccgcagcgcc agcgcgtgac
    18061 aagcccggtg ataccgatca catcggaaca caccgtcttt tcagcccctc gccgcttgcg
    18121 ggagcggggg agaacaacgc atttgcatgg gatttggcag gagtcgtgcg cggcaccgca
    18181 cggcggagcc ggccgcctcg ggagcgggac ccgcgcagcc cgccgtgcag gccgcgggat
    18241 ccgatccgag cgtagccgct gcgggggctg ttcgtgcagc aaggtggtgt aatctggtgc
    18301 agatgctgcc atccggaccc cgccgccatg accgatacct ccaccgcccg cagcacccgc
    18361 gcctcggtga gctttccgcc gacgctctat gaggaactgg tgcgcatcgc tgcgcagaag
    18421 aaggtgtcgg tggcctgggt gatccgcgag gcggcggagc gttatgtcag cgagcgttat
    18481 ccgctgttcg acggccgccc gggcagcggt gagcacgaat gagcgcgctg cctctgagcg
    18541 atgcagacgg cgatgccgcg gccgacctgc gccgcttcgt cagcggccgg tctttcggca
    18601 ccatcctcgc cgacccgccg tggcagtttc agaaccgcac cggcaaggtc gcgcccgaac
    18661 accggcggct gacgcgctac ggcacgctga cgctgccgga aatcaccgcg ctgccggttg
    18721 ccgaagccgc ggccgaggtc tgtcacctgt atctgtgggt gccgaacgcg ctgctgcccg
    18781 agggcctgca ggtcatggcg gcctggggct tccagtacaa gagcaatctc gtctggcaca
    18841 agatcaggaa ggacggcggg ccggacgggc gcggtgtcgg cttctatttc cgcaacgtca
    18901 ccgaactgat cctgttcggc gtgcgcggca agaatgcccg cacgctcgcc cccggccgca
    18961 gccaggtcaa tttcatcgcc agccgcaagc gcgagcattc gcgcaagccc gatgaacaat
    19021 acgagttgat cgaagcctgc agccccggtc cgttcctcga actgtttgcg cgcggcgaac
    19081 gcccgggctg gcaggtgtgg ggcaatcagg ccgatggtga gtattacccg agctgggata
    19141 cctatgcgca ccattcgcag gtgcgggatg aaaccgccgg cgtgccggtc acggacgatt
    19201 aatccggtac gtcatcagcg gattacggat cgtcggctgc aacatcgttg aaatcgacgg
    19261 ccgtctccgc aatgccgatc agcagcagcg gacagggatt gccgacgccg cgctgcacgc
    19321 gcgtcatcag cttgtcccag tgtgtcgttg ccgcaccgaa cttgtcggca acgaactgac
    19381 ccgcccaggc ttcggcaaag gccgacagcg gatcatcact gcgctgcagg cgctggctga
    19441 tattggtgcg ctggcgtggt gtcagcgaca cacccactgc ggccagcgcc tcgaaatccg
    19501 tgatgttccg cgtttcggcg tagcgcctga cgaggctgcg caactgctgc tgcaggcgcg
    19561 atccgcgcgt gacgatgatg ccggcgctga tgccgccttc ggcatgcagg cgcttgaagt
    19621 tttcgaggtc tcgatcgaag aacggatcct tgttgttcca ttcgatttcg agtgcgagcg
    19681 tgccgtttgc gaaagtcttg acgtgatcga tctcatgcga aatcgacgca cgctcgacgc
    19741 cgttcagggt cttgcggatt tcaaagcagg ttttcgtcca gccgcgcccg gcaaggctgc
    19801 gccgcaagcg ctgcgtgccg gcggcctcgc cgccgccgga gccgatgatt tcggtaatcg
    19861 gaatgcggat gtcggcaaga ctcgattcga gttcttcgag cgcctcggga aaatcgatcc
    19921 gcaggatcgc tgcagcatgg gattcgcagc ggatttcaaa accggattcc agcaaacgat
    19981 caagcatggc gtccgggctg tcccgtgatg ctgctgtcgg aagtcatcgg atccatccca
    20041 tgcaccctgt tcccgtgtgt ttccggcaag ttcgtatcga aatctgtccg atcataaaca
    20101 aacgacccgc cgggatctgc acccgcggcg ggtcgcctgc agcgattggt gtcgcgtttc
    20161 agtgcgcccc ggctgccgcc gccccgcccg ccgctccgcc ctgcttcttc ggccgcgcca
    20221 gccagacgac gccgatcagc accaggaaga tcatgcccga cagccagaac agatcatcgg
    20281 cggcgagcag caccgactgc tgcgtgacct ggcgatcgag ggcgccgagg gcctgctgcg
    20341 ggtcgaggcc gaactgctgg gcgtaggtgt tcagcgtctg cgtcgtggtc agcgagtacg
    20401 gcgtcaggtg ctcgacgagc tgcgcgtggt gcagcgcggc gcggtcttcc cactgcgtcg
    20461 tgtacaccga ggccccgatc gcgccggcgg tgatgcgcgc gaagttcgac aggcccgagg
    20521 cggccgggat gcgttccggc ggcaggccgg agagcagcat cgcggtcagc ggcacgaaga
    20581 aggtcgacat cgccgcgccc atcaccagtt gcggcatcgc cagcgtcacg gcgtcggccc
    20641 cggtcgtgaa gcccgagcgc atgaaggcca cgactgcgaa caccacgaac gcgaagctcg
    20701 cgagcaggcg cggatcggtg cgcggcatca ggcgcgcgac cagcggcgtc agcagcacgg
    20761 cgagcacgcc ggtcggggcg gtgacgatgc cggcccaggt cgccgtgtag cccatgtact
    20821 gctgcagcca cagcggcatc agcacgacgt tgccgaagaa cacgccgtag gcgagcgcga
    20881 tcgccagcac gccggtcgtg aagttgcggc ttctgaacag cgacaggtca acgatcgggt
    20941 gcttctcggt cagctcccag accaggaaga acacgaagcc gaccgcggcg gtgatcgcaa
    21001 gccccaggat cagcggcgac gagaaccagt cgagctcctt gcccttgtcg agcatcagct
    21061 gcagcgacgc gacccagagc accagcaggc cgaggccgac gccgtcgatc ggcagcttgc
    21121 gcgtcggcgt ctcgcggccc ttcagcacgc gcgcggtcag cgccgcggcg acgatgccga
    21181 ccggcacgtt gatccagaag atccacggcc acgaggcgtt gtcggtcagc cagccgccga
    21241 gcagcgggcc ggcgaccggc gcgaccagcg tcgtcatcga ccagacgccg agcgccatcg
    21301 gggctttctc cttcgggaag cacgacagca gcagcgcctg cgacagcgga atcatcgggc
    21361 cggcgacgaa gccctgcacg acgcggaaga agatcaggct ctccagcgtc ggcgccagtc
    21421 cgcaggcgag cgaggcgcag acgaaggcga gcaccgaata caggaacagc cgcagctgcc
    21481 cgagccgctg cgtcatccag ccggtcagcg gcacggcgat ggcgttggcg accgcgaacg
    21541 aggtgatgac ccaggtgccc tgcgtcgggc tgacgccgag atccccggcg atcgccggca
    21601 gcgagacatt ggcaatcgtc gtgtcgagca cgttcatgaa ggtcgccgtc gacagcgcga
    21661 cggcggcgac cgcgagcgcg gcaccggtga gcggcggcag cgcggcggtc gcggccgccg
    21721 gcgtcgccgc cgg
//
LOCUS       DAOQIE010000020        21582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1098_length_21582_cov_8.473405, whole genome shotgun sequence.
ACCESSION   DAOQIE010000020 DAOQIE010000000
VERSION     DAOQIE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 21582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21582
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_21582_cov_8.473405"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..506)
                     /locus_tag="PK144_02455"
     CDS             complement(231..506)
                     /locus_tag="PK144_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64617.1"
                     /translation="MLRECAAVIAQRIHAAFPARSPDADHDHVFQALLYFRHFRDAGI
                     EVGIGGILHAVLRDALQTRRMDRLTLQPVQRKEAERRHHRQRRVRRP"
     gene            complement(632..1954)
                     /locus_tag="PK144_02460"
     CDS             complement(632..1954)
                     /locus_tag="PK144_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF64618.1"
                     /translation="MTDLAAPAAPDRLSEAIELHRRGQGLAAEPIYLELLAADPGHPD
                     ALHYLGLLRHQQGRDDEALDLIGRALQAVPRYTDAWNNLGNVHLRAGRFDEAETAYRA
                     ALECDAGHAGAWNNLGLTLRARGLYADSAEAYAKLASLAPDFAEAHYQLGLMLRHTST
                     LASVLDAFRRCIEIDPTHARAHYQYGYMLYITGHTAEAADVFRQWLTFDAANPVPRHM
                     LAACSGEDVPARAADDYVKRTFDDFAESFDEVLLEKLDYHAPDLLAARLRADLGTAQA
                     TRIIVDAGCGTGLCGPWLKPYAQRLIGIDLSPGMLRKADARGDYDKLYEAELTRFLAT
                     YPAAFDVIVSADTLCYFGALEEFAAAARRSLKAGGWLGFTVERLDDDNPEPYRIRPHG
                     RYAHRGDYVQATLAAAGFSGLSLEPGVLRMELSHPVQGWVVRARVGGA"
     gene            complement(2079..2495)
                     /locus_tag="PK144_02465"
     CDS             complement(2079..2495)
                     /locus_tag="PK144_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64619.1"
                     /translation="MKPVRALLAAVLLAAGTVAGAGGEMPAGKRVAPPAVAPLTLHGV
                     RYEAPPDTRDFGDDQAGGYLVARDARSGRELWRQRIYATAYDPKLESDVQDVFIASLR
                     AGRGGRTLEITDERGRRYTLHLGSRAVQEQGVQNRR"
     gene            complement(2492..2842)
                     /locus_tag="PK144_02470"
     CDS             complement(2492..2842)
                     /locus_tag="PK144_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64620.1"
                     /translation="MNDDELEAGLPAPKRVGPPEVEPVTIGNVRYEVVHWGRERGLGQ
                     NGGYLEAFDAASGASLWLLQVYVIDYDPDLEEDVQDVFIESLKTGPRGTLKIVDERGR
                     RFVVDPATRTVTAK"
     gene            complement(2870..3883)
                     /locus_tag="PK144_02475"
     CDS             complement(2870..3883)
                     /locus_tag="PK144_02475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HNF64621.1"
                     /translation="MNKFTEAYRKARDVLENQVFESKWQEFLFADCQARTLFGADGLA
                     VARADDPERVRKRLRDLCKSGNRKIGAVIVEAAQNPVSSGTLAERAATLKMLRHLYHV
                     VKKGGQNVWVYSPPKAYEKWVFDELTGDAKALEAKLNHEKKIFSSTEMRWMASALGVA
                     LKISEDTKAKLSGATGKKDDTDAVIKRWFLDEDSGDAQLTEARTKLLDGFKKIAVACA
                     SDKLVFADYADWIKTRSKYFGAAFRGGEGGGFPVIYLEGAFTRLTGNTGKMWLCAETI
                     IHEFSHHEVSTRDHRYDSSGLKPAKATLPYAKAIDNADSWGYFALDLAGYLSKSDRLN
                     VLK"
     gene            complement(4116..5285)
                     /locus_tag="PK144_02480"
     CDS             complement(4116..5285)
                     /locus_tag="PK144_02480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNF64622.1"
                     /translation="MTTLTPLQAARIARGVYTLLDQTVGEARERGDLFGCEGLFTVGE
                     ESRFTGRSGGLIACKTISGFGYLAEGEGRHQGEILVATRGTSIQLDWLSNLNIGMQIG
                     PGGLPVHAGFHEVWKSFADGLAQYLRGRNPSVIHCVGHSLGGALATLNADYFSHIRAG
                     AVRLYTFGSPRAGSLFFSNALRQRLGAEHIHRVHHAADPVPKIPLFPFLHVPADSAGY
                     PLASGNHGLVSVAAHSMKDSYIPGVGEDNWIGLERRCRDDLNDARVQGWLDQIASGGG
                     HVLPLGARTLQMIGRALAWLLKKTSALLVGTFGTLLTAGMTVLDQLAWLLAKSAELSI
                     ELTGYIAALIKAIFLFLGRTAIVGTVMTFGFVRWVLGLLFGTLTGLARSALSVLG"
     gene            complement(5334..5723)
                     /locus_tag="PK144_02485"
     CDS             complement(5334..5723)
                     /locus_tag="PK144_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF64623.1"
                     /translation="MGNLCLFSAVTGVVLDDGRPVEGARLERSYEWRWKPESGRDETR
                     TDADGRFRLPAITRGSLLGSLLPHEPFVRQTLLIHHAGRTYQAWMFNKGEYAENGELG
                     GRPISLVCRLEAAPEHHGEVYGICEPQ"
     gene            complement(5815..8517)
                     /gene="tssH"
                     /locus_tag="PK144_02490"
     CDS             complement(5815..8517)
                     /gene="tssH"
                     /locus_tag="PK144_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HNF64624.1"
                     /translation="MAEISRVKLFGKLNSLGYKAVEAATVFCKMRGNPYVELVHWVHQ
                     ILQLPDSDLHRLIRQFNVNPSVLVKDLTDALDRLPRGSTSITNLSAHVEEAVERGWVY
                     GSLMFGEAQVRTGYLVVGILKTPSLRSALLGISKEFDKFKADALTDRFDEYVSGSPEQ
                     GLHATDGFQVGGGAAPGEASGAIPPAQLGKQEALNRFTTDLTEQARSGKMDPIVGRDD
                     EIRQVVDILMRRRQNNPILIGEAGVGKTAVVEGFAQRIARGDVPPSLQEVTLRALDVG
                     LLQAGASMKGEFEQRLRSVIEEVQASPKPVILFVDETHTLVGAGGAAGTGDAANLLKP
                     ALARGTLRTIGATTFAEYKKHIEKDPALTRRFQSVQVDEPDEKKAVLMMRGVASTMEA
                     HHKVQILDEALEAAVKLSHRYIPARQLPDKSVSLLDTACARVAVSLHAVPAEVDDSRK
                     RIEALDTELRIIAKEAAVGLDTAARESLCNERLAAEKARLEGLEARWNAEKALVDELL
                     ATRAALRANIGVVDGPAKGEPPAKLGEAEVAALREKLHGLQTQLAELQGEQPLILPTV
                     DYQAVASVVEDWTGIPVGRMARNEIETVLKLADTLGQRVIGQDHAMQMIARRIQTSRA
                     GLDNPNKPIGVFMLAGTSGVGKTETALALAEALYGGEQNLITINMSEYQEAHTVSSLK
                     GAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHPDVHEIFFQVFDKGWMEDGEGR
                     LIDFKNTLILLTTNAGTDLLMQLCKDPELMPEPDDIAKALREPLLKVFPPALLGRLVT
                     IPYYPLSDEMLGRIVRLQLGRIKKRVEEHRKVPFEYDDEVVKLIVSRCTELESGGRMI
                     DAILTNTMLPDISREFLERMMEGQPVTRVHVSVRDGEFAYAFDA"
     gene            complement(8594..9703)
                     /gene="tssG"
                     /locus_tag="PK144_02495"
     CDS             complement(8594..9703)
                     /gene="tssG"
                     /locus_tag="PK144_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNF64625.1"
                     /translation="MNADADTEDSGIAPALSAADFGVAPPVLLSPVAEALFTALAAKP
                     WDFDLWQALRRIECAFPDKPRLGRSFRVADDPVRLSQPPHTTFVPSTIAAFERPSHRP
                     ARLASYAFGLFGPHGPLPLHLSEHARERQRNHRDPTFARFADIFHHRLMSLFWRAWAD
                     AQPTVGMDRPGEDRFARYVGSLAGYGDEALRDRDVLGHRPRLHFAGLLGPQARHPDGL
                     RAILADYLGLPVRIGEYIGHWLELPPPVQCRLGDSRASGTLGVNAVIGTRVWDRQYKF
                     RVVLGPLTLADYERLLPGGASLARLRAWVRSYVGEEYAWDARLILARPEVPEARLGGP
                     TRLGWTTWIGTPGAADPADLQLQESVMTAPVPAPA"
     gene            complement(9700..11577)
                     /gene="tssF"
                     /locus_tag="PK144_02500"
     CDS             complement(9700..11577)
                     /gene="tssF"
                     /locus_tag="PK144_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNF64626.1"
                     /translation="MDPRLYRYYNQELQHLRETAGEFAQEFPKIASRLSLTGFECADP
                     YVERLLEGCAFLSARVQLKLDAEFPVFTQHLLEMVYPHYLAPVPSMAVLQLQPDLSEG
                     SLAAGFTVPRGASLKSVLGKNETTACEYRTGQPVTLWPVELTEAEYFGNVTAVLGPAA
                     NSAPRARAGLRLRLRATAGLSFDALSLERLPLFLRGADQLPFRLYEQLLGNAVAVLAR
                     PAQRPAPWFQWLGRESLAPMGFEDEQALLPLSPQSFQGYRLLGEYFAFPQRFLFVELR
                     ALAPVVRRCSGSELELLVLFDRLDPMLEGVLKPENFVLHCAPAINLFPRRADRIHLND
                     RSYEYHIVPDRTRPLDFEVHSLERVTGHGTTEQAEQAFLPFYCAHDRIRRAADGAYYT
                     LRRTKRLLSARAKLRGPRSGYIGQEVFIGLVDAAAAPYRHDLRQLSLEVLCTNRDLPL
                     TMPLGLGRTDFTLESGAPVAAVRCVAGPTRPRPSNAENDVAWRLISHLSLNYLTLADS
                     DAQQGAVALRELLRLYVDPNDAAGQKQVDGLLSVKTQPATRRLPLPGPIAFGRGVEIA
                     LNFDEGAYGGAGVFLMGAVLERFLGRYVSMNSYTETSVRSLERGEIMRWPVRMGARAL
                     L"
     gene            complement(11595..12098)
                     /gene="tssE"
                     /locus_tag="PK144_02505"
     CDS             complement(11595..12098)
                     /gene="tssE"
                     /locus_tag="PK144_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNF64627.1"
                     /translation="MAELTPQERLQPALLDRLIDDAPERHKETREQRVLSLNQLRESV
                     LRDLAWLFNAGNHESVQPLDAWPQVARSVLNYGLPALSGRTASSVDIPALERVLADTI
                     RTFEPRILPHTLQVRAVVAREQMNHNAIGFEIQGELWAQPVPLHLWLKTEIDLESGNV
                     RIEPRNA"
     gene            complement(12107..12910)
                     /locus_tag="PK144_02510"
     CDS             complement(12107..12910)
                     /locus_tag="PK144_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HNF64628.1"
                     /translation="MSVEAALQSGNPREALTELQARVRKEPAVARHRVLLFQLLCVLG
                     QWPRALGQLQVVGELDAEALPMVQTYREALRCEVFRQEVFAGRRAPLLFGEPTPWVAL
                     LIEALARDADDPQAAAGLRAEAFERAPANPGSADGRPFAWLADTDGRLGPVIEAIING
                     RYYWIPCERLSSVQQEAPADLRDVVWMPASLTFTTGGETVALLPARYPGLDAEDDGGL
                     LLGRRTEWRENEHGLVLAAGQRVFATDGDDVALYDLRTLHFDPAAPASA"
     gene            complement(13050..13544)
                     /locus_tag="PK144_02515"
     CDS             complement(13050..13544)
                     /locus_tag="PK144_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNF64629.1"
                     /translation="MAVDIFLKFADAANIKGESKDKKHPKEIDVLAWSWGMSQSGTFH
                     TGGGGGAGKVNVNDMSVTKWVDIASYALMHACATGQHIDDALLTVRKAGGKEPVEYIK
                     IKMEKVMVTSINTGGSGGEDRLTENVSLNFAKVTFTYTEQTDKGAGEASKDFKWDIEA
                     NAVP"
     gene            complement(13653..15149)
                     /gene="tssC"
                     /locus_tag="PK144_02520"
     CDS             complement(13653..15149)
                     /gene="tssC"
                     /locus_tag="PK144_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNF64630.1"
                     /translation="MAELNAEAQAAGAGLTTEGGAFEALLLQEFKPKSDRAKEAVEEA
                     VRTLAEQALASSSIVSGDTLKTIEAMIAALDAKLTEQINHILHHADFQKLESAWRGLH
                     HLVNNTETDEMLKIRVMNISKQDLHKTLKKFKGTAWDQSPIFKKLYEEEYGQFGGEPY
                     GCLVGDYYFDHSAPDVELLGEIAKVSASMHAPFIAAVAPAVMQMDSWQELSNPRDLTK
                     IFQTPEYAAWKSLRESEDSRYVGLTMPRFLSRMPYGAKTNPVDEFDFEEDTTGADSNK
                     YTWANSAYAMAVNINRSFKLYGWCSRIRGIESGGAVEGLPAHSFPTDDGGVDMKCPTE
                     IAISDRREAELAKNGFMPLVHKKNTDFAAFIGAQSLQKPAEYDDPDATSNANLAARLP
                     YLFATCRFAHYLKCMVRDKVGSFKEREDMQIWLQKWIMNYVDGDPSRSSETTKAQKPL
                     AAAEVVVEEVEGNPGYYTSKFFLRPHYQLEGLTVSLRLVSKLPSQKGG"
     gene            complement(15168..15668)
                     /gene="tssB"
                     /locus_tag="PK144_02525"
     CDS             complement(15168..15668)
                     /gene="tssB"
                     /locus_tag="PK144_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNF64631.1"
                     /translation="MADSSQKFIARNRAPRVQIEYDVELYGAQKKVQLPFVMGVMADL
                     SGKPAEPLGPVAERKFLEIDVDNFDSRMKSMKPRVAFAVPNTLTGDGNLSVDITFESM
                     EDFTPAAIARKVDALNKLLEARTQLSNLLTYMDGKTGAEELIGKVLQDPSLLQALASA
                     PKPQSE"
     gene            complement(15739..16818)
                     /gene="tssA"
                     /locus_tag="PK144_02530"
     CDS             complement(15739..16818)
                     /gene="tssA"
                     /locus_tag="PK144_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNF64632.1"
                     /translation="MALDVEKWLEPVSDDEPGGSDLEYDPDFQALERLATGKPEQQIG
                     STIVPAEPPDWRAVRGKAMELLERTRDLRVALVLTRALLAVEGLPGFADGLRYLHGLL
                     EHHWEHVHPQLDPDDYNDPTTRVNIIAGLVAADSDDVLGLLRQAALARSRSFGVATLR
                     DWLILSGASSGTPVLTTETFDAVFREVAAEELQAAADSAHAAFDLASAIERRLTEQVG
                     SGNAVDLSPLRTALKPCVQLLDERLAQRGLGAVAETEALPVAGEGPAEAGGSAAAGAG
                     ASGFVAGRISTRDDVLKALEAVCDYYTRHEPSSPVPILMERARRLVTMSFVDIMRNIA
                     PDSLAQIDVLRGPDPDAESSSSDGY"
     gene            17025..18752
                     /gene="tagH"
                     /locus_tag="PK144_02535"
     CDS             17025..18752
                     /gene="tagH"
                     /locus_tag="PK144_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HNF64633.1"
                     /translation="MPLTLRITSQHPLLPEADTVRSLGSGDSLTIGRAPGNDWVLPDP
                     ELVISGRHCTVHEEQGRYVLTDTSTNGVYLGGQRVGRGNSRPLQAGDVIAIGEYQIAV
                     EPGAGDGGQAPAFGAGTAAAVALPDAFGLAAPAPLADPFGLHGTPGAQDPFELHGKPA
                     GEDPFGLRAPATGDDPFGLGNDLSGGDPFGLIGTPGTSGSARGGGAPAAADPFGLAGG
                     GDRNPAGADPFGLGGGAGRPDFAAPATPAGPATAEAGGDPFGLLAPAPLTSAPVPDHV
                     PSEQQAFVPPAPVSDDDWDALFPAPAAAGAAPVRLDIPPAAKTPDPLFAGLGPLAEES
                     APPAAPPPDETPAVPAFTPAPVSGPVPAPAPAPIPVPAPAPATPAAAAPAAELMAALL
                     EGAGLAHLQIPQARQADLLRALGDSYRRMVEGLMTVLRARSHVKGEFRMQQTSIRPIE
                     NNPLKFAPNVEEAMTLLLTLRSQSYLSPERAVAEAFEDLQAHQLAMMAGMQAALGHLF
                     RRFDPATLEARFGSGGLLPGSRKARCWEQFTALYQDIAREAEDDFQELFGREFVRAYE
                     EQIARLRSR"
     gene            18838..19302
                     /gene="tssJ"
                     /locus_tag="PK144_02540"
     CDS             18838..19302
                     /gene="tssJ"
                     /locus_tag="PK144_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HNF64634.1"
                     /translation="MNLRHGLSALGLLVLLGACAAPAPPPPTRISGQIEAATPLNPGP
                     GGGSLPLRVRLYELKNPGTFQSADFFSVWDNPQAKLGADLLGTEDYTLHAGARQPFER
                     TLDPQARYVGFVAAYRDLNGAVWRQSLAVPANQTTRLRVQAGTKTLSVGAGQ"
     gene            19352..20686
                     /gene="tssK"
                     /locus_tag="PK144_02545"
     CDS             19352..20686
                     /gene="tssK"
                     /locus_tag="PK144_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HNF64635.1"
                     /translation="MSWHSKVVWSEGMFLRPQHFQQQERHLDRVLQGVCGALEPYVWG
                     FAGLALDRQALTLGRIAIASARGVLPDGTPFSIPDDDAAPPPLAVPDDLRDARVVLAL
                     PLRRPGLRDTARDGEDAAITRYRTEIAEVRDNNAGLESLAPVELARHNLRLLTDRQDL
                     GEYACLGMVHVIEKKTDQSVVLDERYVPPVLDALQQPVLAALVREIEGLLHQRAEALA
                     GRVVASGRGGVAEIADFLLLQAVNRAEPLFTHLARVRALHPEPLYRELLGLAGELATF
                     TRPNKRPPEFPAYDHDDLAATFAAVMAEIRQALSMVLEQNAIQIPLQERIAGVHVGVI
                     TDRNLIGKASFVLAVRADVPGEILRARFPTQVKIGPVEHIRDLVTRQLPGIGLNPLPV
                     APRQIPYHAGLTYFELDPASELWKSLASSGGFAFHVAGQFPGLALTFWAIRS"
     gene            20709..>21582
                     /gene="icmH"
                     /locus_tag="PK144_02550"
     CDS             20709..>21582
                     /gene="icmH"
                     /locus_tag="PK144_02550"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HNF64636.1"
                     /translation="MVDPNDPFAGLGGDTGDRTQVLKPRPGGARAPLPQGAPSAANAV
                     PSYAPPPPPAPAAPVEALRFGGGINALERAAAPLLALVHGLRNSVSHPDPLGLRRRVA
                     EEIRSFEARARADGVRQEEIFAARYVLCTLLDETAMATPWGASSGWSAQSLLVQFHNE
                     AWGGEKVFQLLEKLLQDPRSNLNLLELVYVVLALGFEGKYHVLPDGRNQLEALRERLY
                     QTLRTVRGEFERELSVHWRGAPVKRNPLSRYVPLWALGALAASLLLGLFLILRASLNA
                     EAEPVYANLHGLGEN"
BASE COUNT         3134 a         7520 c         7508 g         3420 t
ORIGIN      
        1 gcaaggttgt gaaggaattg tcggccgagt gcgaagtcag cgccgcagcg atgaaccgct
       61 atttcgaagc cagccactga agcctttcgt cccggcactc atttttccct gatttccggg
      121 cactgccggc gcggatccgc catccgcgcc acggcgaccg tccaaccggc attgcggagt
      181 ggctgcttcg ggtcagcctt gctccggcgg cgtatcgttg cgcacgatgc tcagggtcgg
      241 cgcacgcgac gctgccggtg atgccgccgc tccgcctctt tccgctgcac cggctgcagc
      301 gtcagcctgt ccatgcgacg ggtctgcaag gcatcgcgca ggacggcgtg aaggattccg
      361 ccgatgccga cctcgatgcc ggcatcgcga aagtgcctga aatacaaaag tgcctgaaat
      421 acatgatcgt gatctgcgtc cgggcttcgg gccggaaacg cagcatgaat ccgctgcgca
      481 atgactgctg cgcattcgcg aagcattcct cgatcagccg tgccggttcg tcgatttccg
      541 gatggcgcag cgccagacag gcgccaatct gccggcgcag ctcctcgggc ggcgtagccg
      601 gtctccgcgg caaggacccg gggccgggaa ttcaggcgcc gccgacccgc gcacgcacga
      661 cccagccctg caccggatgt gacagctcca tgcgcagcac gcccggctcc agcgacagcc
      721 cgctgaatcc ggctgcggcc agcgtcgcct gcacgtagtc gccgcgatgc gcataccggc
      781 cgtgcgggcg gatgcggtat ggctcgggat tgtcgtcgtc cagccgctcg accgtgaagc
      841 cgagccagcc gccggccttc aggctccggc gcgcagcagc ggcgaattcc tccagcgcgc
      901 cgaagtagca cagcgtgtcg gccgagacga tcacgtcgaa ggccgccggg taggtggcga
      961 gaaagcgcgt cagctcggct tcgtacagct tgtcgtagtc gccgcgggca tcggccttgc
     1021 gcagcatgcc gggcgacagg tcgatgccga tcaggcgctg cgcgtagggc ttcagccacg
     1081 gcccgcacag cccggtgccg cagccggcat cgacgatgat gcgcgtggcc tgcgcggtgc
     1141 cgagatcggc cctgagccgt gccgcgagca gatccggcgc gtgatagtcg agcttttcga
     1201 gcagcacctc gtcgaagctc tcggcgaagt catcgaaggt gcgcttcacg tagtcgtcgg
     1261 cggcgcgcgc cggcacgtcc tcgcccgagc acgcggcgag catgtggcgc ggcaccggat
     1321 tggccgcatc gaaggtcagc cactggcgga acacgtcggc ggcctcggcg gtgtggccgg
     1381 tgatgtacag catgtagccg tactgatagt gcgcgcgggc gtgggtcgga tcgatctcga
     1441 tgcagcggcg gaaggcatcg agcacgctcg cgagcgtcga ggtgtggcgc agcatcaggc
     1501 cgagctggta gtgcgcctcg gcgaagtccg gcgccagcga cgcgagcttc gcgtaggcct
     1561 cggccgaatc ggcgtacagg ccgcgcgcgc gcagcgtcag gccgaggttg ttccaggcgc
     1621 cggcgtgtcc ggcgtcgcat tcgagcgcgg cacggtaggc ggtttcggcc tcgtcgaaac
     1681 gcccggcgcg cagatgcacg ttgccgaggt tgttccaggc gtcggtgtag cgcggcacgg
     1741 cctgcagcgc ccggccgatc aggtcgagcg cctcgtcatc gcgcccctgc tggtggcgca
     1801 gcaggccgag gtagtgcagc gcatccgggt ggccgggatc ggcggccagc agttcgagat
     1861 agatcggttc ggcggcgagg ccctgcccgc gccggtgcag ttcgatggct tcggacagac
     1921 ggtctggggc ggccggggcg gcgagatcgg tcatcggaac tccggaagcg ggacggcggc
     1981 ggacggtgcg gggttcagcg tataccgccg gccgccgccg cgacgaccgg ccggccgatg
     2041 cgcgccgcag gaacccggcg ccggcctcgg gccgggattc agcgccggtt ttggactccc
     2101 tgctcctgca cggcccggct gcccaggtgc agcgtgtagc gccgtccgcg ctcatcggtg
     2161 atttccaggg tccggccgcc gcgcccggcg cgcagcgagg cgatgaacac gtcctgcacg
     2221 tcggattcca gcttcgggtc gtaggcagtc gcatagatgc gctggcgcca gagttcgcgg
     2281 ccgctgcggg cgtcgcgggc gacgaggtag ccaccggcct gatcgtcgcc gaaatcgcgc
     2341 gtgtcgggcg gtgcctcgta gcgcaccccg tgcagcgtca gcggcgcaac ggccggcggc
     2401 gccacgcgct tgccggccgg catttccccg cccgcaccgg cgaccgtgcc cgctgccagc
     2461 agcacagccg cgagcagcgc gcgcaccggc ttcacttcgc cgtcaccgtg cgcgtggccg
     2521 gatcgacgac gaagcgccgg ccgcgctcgt cgacgatttt cagcgtgccg cgcggcccgg
     2581 tcttcaggct ctcgatgaac acgtcctgca cgtcctcttc gagatcgggg tcatagtcga
     2641 tcacgtagac ctgcagcagc cacagcgacg caccgctcgc cgcatcgaag gcttccaggt
     2701 aaccgccgtt ctgcccgagc ccgcgctcgc gcccccagtg cacgacctcg tagcgcacgt
     2761 tcccgatcgt gaccggttcc acctccggcg gcccgacccg tttcggcgcg ggcaggccgg
     2821 cctccagttc gtcatcgttc atggctgttc tcccgaaaag cggaccgcat cacttcagca
     2881 cgttgagacg gtcggacttc gacaggtaac cggccagatc gagggcgaaa tagccccagc
     2941 tgtcggcatt gtcgatggcc ttggcataag gcagcgttgc cttggcaggt ttcaggccgc
     3001 tgctgtcgta gcggtgatcg cgggtgctga cttcgtggtg cgagaactca tggatgatgg
     3061 tttccgcaca cagccacatc ttgccggtat tgccggtcag gcgcgtgaag gcgccttcga
     3121 gatagatcac cggaaaaccg ccgccctcgc cgccgcggaa ggcggcaccg aaatacttgc
     3181 tgcgggtctt gatccagtcg gcgtaatcgg cgaacaccag cttgtcggag gcacaggcga
     3241 ccgcgatctt cttgaaaccg tcgagcagtt tggtgcgtgc ctcggtcagt tgcgcatcgc
     3301 cgctgtcctc gtcgagaaac cagcgcttta tcacggcatc ggtgtcgtcc ttcttgccgg
     3361 tggcgccgct gagcttcgcc ttggtgtctt cggaaatctt cagcgccacc ccgagtgccg
     3421 aggccatcca gcgcatttcg gtgctggaga agattttctt ctcgtgattg agcttggcct
     3481 ccagcgcctt ggcatcaccg gtcagttcgt cgaacaccca tttctcgtag gccttcggcg
     3541 gtgaatacac ccagacgttc tggccgccct tcttcacgac gtgatagagg tgcctgagca
     3601 tcttcagcgt cgctgcgcgc tcggcgagcg tgccggagga aaccggattc tgcgcggcct
     3661 cgacgatcac cgcgccgatc ttgcggtttc cggacttgca gagatcgcgc aggcgcttgc
     3721 gcacgcgctc cgggtcgtcg gcgcgggcca cggccagtcc gtcggcgccg aacagcgtcc
     3781 gcgcctgaca gtcggcaaac aggaactcct gccacttgct ttcgaacacc tgattttcga
     3841 gcacgtccct ggcctttctg taggcttccg tgaacttgtt catgctcgcg ctcctcccgg
     3901 gagacctgag ttttctggcc ggggcagcgg attgctgctg ccggactccg gccgttcgtg
     3961 cggcagcccc ctgcggtgac tgccgcggaa tgccacgcga accaccccgg tacgtggcgc
     4021 ggatggccgg ccgcgtgccc tcggggccgg ccggcagggt cgctgaagca ggtctgcaaa
     4081 cccggccgat gccgcaaggc cgccggccgg cccgctcagc cgagcaccga cagcgccgag
     4141 cgggccagcc cggtcagcgt gccgaacagc agccccagca cccagcgcac gaagccgaag
     4201 gtcatgaccg tgccgacgat cgcggtgcgg ccgaggaaca ggaagatggc cttgatcagg
     4261 gccgcgatgt agccggtcag ctcgatcgac agctccgcac tctttgcgag cagccaggcg
     4321 agctggtcga gcacggtcat gccggcggtg agcagcgtgc cgaaggtgcc gaccagcagg
     4381 gcgctggtct tcttcagcag ccaggcgagc gcccggccga tcatctgcag cgtccgggcg
     4441 ccgagcggca gcacgtgacc gccgcccgag gcgatctggt cgagccagcc ctgcacgcgg
     4501 gcatcgttga ggtcgtcgcg gcagcgccgc tccagcccga tccagttgtc ctcgccgacg
     4561 ccggggatgt agctgtcctt catcgaatgc gcggcgacgc tgaccagacc gtggttgccg
     4621 ctggcgagcg gatagccggc gctgtcggcc ggcacgtgca ggaacgggaa cagcgggatc
     4681 ttcggcaccg ggtccgcggc gtggtggacg cggtggatgt gctcggcgcc gagccgctgg
     4741 cgcagcgcgt tcgaaaagaa cagcgagccg gcacgcggcg agccgaaggt gtacaggcgc
     4801 acggcgccgg cgcggatgtg cgagaagtaa tcggcgttca gcgtcgcgag cgcgccgccc
     4861 aagctgtggc cgacgcagtg gatcaccgac gggttgcggc cgcgcaggta ttgcgcgagc
     4921 ccgtcggcga aggacttcca gacctcatgg aaacccgcat gcaccggcag cccgcccggg
     4981 ccgatctgca tgccgatgtt caggttcgac agccagtcga gctggatgct ggtgccgcgg
     5041 gtcgcgacga ggatctcgcc ctggtgacgc ccctcgccct cggcgaggta gccgaaaccg
     5101 ctgatggtct tgcaggcgat caggccgccg gagcggccgg tgaagcgcga ttcctcgccg
     5161 acggtgaaca ggccctcgca gccgaacagg tcaccgcgtt cgcgggcctc gccgacggtc
     5221 tggtcgagca gcgtgtagac gccgcgggca atgcgggccg cctgcagcgg cgtgagcgtc
     5281 gtcatggtct tggcctcttg gagcgggcgc cgcgggtggc gcgctgctgc ggctcattgc
     5341 ggctcgcaga tgccgtacac ctcgccgtgg tgttccggcg ccgcttcgag ccggcacacc
     5401 agcgagatcg gccggccgcc cagttcgccg ttctcggcgt attcgccctt gttgaacatc
     5461 cacgcctggt aggtccggcc ggcgtggtgg atgagcaggg tctggcgcac gaagggttcg
     5521 tgcggcagca gcgagccgag cagcgagccg cgcgtgatcg ccggcaggcg gaagcgcccg
     5581 tcggcgtcgg tgcgggtctc gtccctgccg ctttcgggct tccagcgcca ttcgtagctg
     5641 cgttcgagcc gcgcgccctc gaccggccgg ccgtcatcga gcacgacgcc ggtgacggcg
     5701 gagaacaggc acaggtttcc catcgcgatg gcctcgtgag cgtgaatcag caacagcgtc
     5761 agcagcggcc cgccccggcg acgatgcctg cggggcgggc acaccgcggg ccgctcaggc
     5821 atcgaaggca tacgcgaatt cgccgtcgcg caccgacacg tgcacgcgcg tgaccggctg
     5881 gccttccatc atgcgttcga ggaactcgcg gctgatgtcc ggcagcatgg tgttggtcag
     5941 gatcgcatcg atcatgcgtc cgcccgattc cagctcggtg cagcgcgaga cgatgagttt
     6001 gacgacctcg tcgtcgtact cgaacggcac cttgcggtgc tcctcgacgc gcttcttgat
     6061 gcgcccgagc tgcagccgga cgatgcgccc gagcatctcg tcgctgagcg ggtagtacgg
     6121 gatcgtcacc agccgcccga gcagcgccgg cgggaacacc ttcagcaggg gttcgcgcag
     6181 cgccttggcg atgtcatcgg gctcgggcat cagctccggg tccttgcaga gctgcatcag
     6241 caggtcggtg ccggcgttgg tggtcagcag gatcagcgtg ttcttgaagt cgatcagccg
     6301 gccctcgccg tcctccatcc agcccttgtc gaagacctgg aagaagatct cgtgcacgtc
     6361 cgggtgggcc ttctcgacct cgtcgagcag cacgaccgag tacggccgcc ggcgcacggc
     6421 ctcggtcagc acgccgccct cgccgtagcc gacgtagccc ggaggcgcgc ccttcagcga
     6481 cgagacggtg tgcgcctcct ggtactcgct catgttgatc gtgatcaggt tctgctcgcc
     6541 gccgtagagc gcctcggcga gcgccagcgc ggtctcggtc ttgccgacgc cggaggtgcc
     6601 ggcgagcatg aacacgccga tcggcttgtt cggattgtcg aggccggcgc gcgaggtctg
     6661 gatgcgcctg gcgatcatct gcatcgcgtg gtcctggccg atcacgcgct ggccgagcgt
     6721 gtcggcgagc ttcagcaccg tctcgatctc gttgcgcgcc atgcgcccga ccgggatgcc
     6781 ggtccagtcc tcgaccaccg aggcgaccgc ctggtaatcg acggtcggca ggatcagcgg
     6841 ctgctcgccc tgcagctcgg cgagctgcgt ctgcaggccg tgcagcttct cgcgcagcgc
     6901 cgcgacctcg gcctcgccga gcttcgccgg cggctcgcct ttggccggcc cgtcgacgac
     6961 gccgatgttg gcgcgcagcg cggcgcgcgt cgcgagcagc tcatcgacca gcgccttctc
     7021 ggcgttccag cgcgcctcca gcccttcgag ccgggccttc tcggccgcca gccgctcgtt
     7081 gcagagcgat tcgcgcgccg cggtgtcgag gccgaccgcg gcttccttcg cgatgatgcg
     7141 cagctccgta tcgagcgcct cgatgcgctt gcggctgtca tcgacctcgg ccggcaccgc
     7201 gtgcaggctg accgcgacgc gcgcgcaggc ggtgtcgagc aggctcacgg acttgtccgg
     7261 cagctggcgc gccgggatgt agcggtgcga gagcttgacc gcggcctcca gcgcctcgtc
     7321 gaggatctgc accttgtggt gcgcctccat cgtcgaggcg acgccgcgca tcatcagcac
     7381 ggccttcttc tcgtcgggct catcgacctg cacgctctgg aagcgccggg tcagcgccgg
     7441 gtccttctcg atgtgcttct tgtactcggc gaaggtggtc gcgccgatcg tgcgcagcgt
     7501 gccgcgcgcc agcgcgggct tgagcaggtt ggcggcatcg ccggtgccgg ccgcaccgcc
     7561 ggcgccgacc agcgtgtgcg tctcgtcgac gaacaggatc accggcttcg ggctggcctg
     7621 cacttcctcg atcaccgagc gcaggcgctg ctcgaactcg cccttcatgc tggccccggc
     7681 ctgcagcagg ccgacatcga gcgcgcgcag cgtgacttcc tgcagcgacg gcggcacgtc
     7741 gccacgggcg atgcgctgcg cgaagccctc gacgaccgcc gtcttgccga cgccggcctc
     7801 gccgatcagg atcgggttgt tctggcggcg gcgcatcagg atgtcgacga cctggcggat
     7861 ttcatcgtcg cgcccgacga tcgggtccat cttgccgctg cgcgcctgct cggtcagatc
     7921 ggtcgtgaag cggttcagcg cctcctgctt gccgagctgc gccggcggga tcgcaccgct
     7981 cgcctcgccc ggcgccgcac cgccgccgac ctggaaaccg tcggtcgcgt gcaggccctg
     8041 ctcgggcgag ccggaaacgt attcgtcgaa gcggtcggtc agcgcgtcgg ccttgaactt
     8101 gtcgaactcc ttcgagatgc cgagcagcgc gctgcgcagc gacggcgtct tcaggatgcc
     8161 gaccaccaga tagccggtgc gcacctgcgc ctcgccgaac atcaggctgc cgtagaccca
     8221 gccgcgctcg acggcttcct cgacgtgcgc cgacaggttg gtgatcgagg tcgagccgcg
     8281 cggcagccgg tcgagggcat cggtcaggtc cttgaccagc accgaggggt tgacgttgaa
     8341 ctgcctgatc agcctgtgca ggtcggaatc gggcagctgc aggatctgat ggacccagtg
     8401 caccagctcg acgtacggat tgccgcgcat cttgcagaac accgtcgccg cctcgaccgc
     8461 cttgtagccg aggctgttga gcttgccgaa cagcttgacc cgactgattt ccgccatgac
     8521 gtgctccctc gaaggtggtg gatcgtgttg tatgcggccc cggcgtgccg gcgcgggccg
     8581 cggaggtccg gattcaggcg ggcgccggga ccggcgccgt catgacgctt tcctgcagct
     8641 gcaggtcggc cggatcggcc gcccccggcg tgccgatcca ggtggtccag ccgagccggg
     8701 tcggaccgcc gagccgtgcc tcgggcacct cgggccgcgc gaggatcagc cgcgcatccc
     8761 aggcgtattc ctcgccgacg tagctgcgca cccaggcgcg cagccgcgcg agactcgcgc
     8821 cgcccggcag caggcgttcg tagtcggcga gcgtcagcgg cccgagcacg acgcggaact
     8881 tgtactggcg gtcccagacc cgggtgccga tcacggcgtt gacgccgagc gtgccgctcg
     8941 cgcgcgaatc gccgagccgg cactgcaccg gcggcggcag ttccagccag tggccgatgt
     9001 attccccgat gcgcaccggc aggccgagat agtcggcgag gatcgcgcgc aggccgtccg
     9061 ggtggcgcgc ctgcggcccg agcaggccgg cgaagtgcag ccgcggccgg tggccgagca
     9121 cgtcgcgatc gcgcagggct tcgtcaccgt agccggcgag gctgccgacg tagcgcgcga
     9181 agcggtcctc gccggggcgg tccatgccga cggtcggctg cgcatcggcc caggcgcgcc
     9241 agaacagcga catcagccgg tgatggaaga tgtcggcaaa gcgcgcgaag gtcggatcgc
     9301 ggtggttgcg ctggcgctcg cgcgcgtgct cgctcaggtg cagcggcagc ggcccgtgcg
     9361 ggccgaacag cccgaaggca tagctcgcca gccgggccgg ccggtgggac ggacgctcga
     9421 aggcagcgat cgtcgagggc acgaaggtcg tgtgcggcgg ctgcgacagc cgcaccggat
     9481 catcggcaac ccggaacgag cgcccgagcc gcggcttgtc cgggaacgcg cattcgatgc
     9541 gccgcagcgc ctgccagaga tcgaaatccc acggcttcgc cgccagcgcc gtgaacagcg
     9601 cctcggcgac cggcgacagc agcaccggcg gcgcgacgcc gaaatcggcg gcgctcagcg
     9661 ccggcgcaat gccggaatcc tcggtgtcgg cgtcggcgtt cacagcagcg cccgcgcgcc
     9721 catgcgcacc ggccagcgca tgatctcgcc gcgctccagc gaacgcacgc tggtctcggt
     9781 gtagctgttc atcgacacgt agcggccgag gaagcgctcc agcaccgcgc ccatcaggaa
     9841 cacgccggcc ccgccgtagg cgccctcgtc gaagttcagc gcgatctcga cgccacgccc
     9901 gaaggcgatc ggccccggca gcggcaggcg gcgggtcgcc ggctgcgtct tcaccgacag
     9961 caggccgtcg acctgcttct ggccggcggc atcgttcgga tcgacgtaca ggcgcagcag
    10021 ctcgcgcagc gccaccgcgc cctgctgcgc atcgctgtcg gcgagcgtca ggtagttcag
    10081 cgacaggtgc gagatcagcc gccaggccac gtcgttctcg gcgttgctcg ggcgcgggcg
    10141 cgtcggaccg gccacgcagc gcaccgccgc caccggcgcg ccgctctcca gcgtgaagtc
    10201 ggtgcgcccg agcccgagcg gcatcgtcag cggcaggtcg cggttggtgc acagcacttc
    10261 gagcgacagc tggcgcagat cgtgccggta cggtgcggca gcggcatcga ccagcccgat
    10321 gaacacctcc tggccgatgt agccgctgcg cggcccgcgc agcttcgcgc gcgccgacag
    10381 caggcgcttg gtgcgtcgca gcgtgtaata cgccccgtcg gcggcgcgac ggatgcggtc
    10441 gtgcgcgcag tagaacggca ggaaggcctg ctcggcctgc tcggtcgtgc cgtgaccggt
    10501 cacgcgttcg agcgagtgga cctcgaaatc gagcggccgc gtgcggtccg ggacgatgtg
    10561 gtactcgtag ctcctgtcgt tcaggtggat gcggtcggcg cggcgcggga acaggttgat
    10621 cgccggtgcg cagtgcagca cgaagttctc gggcttcagc acgccttcga gcatcgggtc
    10681 gagccgatcg aacagcacca gcagttcgag ttcgctgccg ctgcagcggc gcacgaccgg
    10741 tgccagggcg cgcagttcga cgaacaggaa acgctgcggg aacgcgaagt actcgccgag
    10801 cagccggtag ccctggaagc tctgcggcga cagcggcagc agcgcctgtt cgtcctcgaa
    10861 gcccatcggc gccagcgatt cgcgaccgag ccactggaac cacggcgccg gacgctgcgc
    10921 cgggcgggcg agcacggcga cggcgttgcc gagcagctgt tcgtacagcc ggaacggcag
    10981 ctggtcggcg ccgcgcagga acagcggcag gcgctcaagc gacagcgcat cgaagctcag
    11041 gccggccgtg gcccgcaggc gcaggcgcag gccggcacgc gcacgcggcg cgctgttcgc
    11101 ggccgggccg agcacggccg tgacgttgcc gaagtactcg gcctcggtca gctcgaccgg
    11161 ccacagcgtc accggctgac cggtgcggta ttcgcaggcg gtggtctcgt tcttgccgag
    11221 cacgctcttg agactcgcgc cgcgcggcac cgtgaagccg gccgcgagcg agccttcgga
    11281 caggtccggc tgcagttgca gcaccgccat cgacggcacc ggcgccagat agtgcgggta
    11341 gaccatctcc agcaggtgct gcgtgaacac cgggaactcg gcatcgagct tcagctgcac
    11401 gcgcgccgac aggaaggcac agccttcgag caaccgctcg acgtaggggt cggcgcattc
    11461 gaagccggtc aggctcaggc gcgaggcgat cttcgggaat tcctgcgcga actcgccggc
    11521 ggtctcgcgc aggtgctgga gttcctggtt gtaataccgg tacaggcgcg gatccatcgg
    11581 ggggctgctt gcgatcaggc gttgcggggc tcgatgcgga cgttgccgga ttcgaggtcg
    11641 atctcggtct tcagccacag atgcagcggc accggctgcg cccagagttc gccctggatc
    11701 tcgaagccga tggcgttgtg gttcatctgc tcgcgggcga cgacggcgcg cacctgcagc
    11761 gtgtgcggca ggatgcgcgg ttcgaaggtg cggatggtat cggcgagcac gcgttcgagc
    11821 gccgggatgt cgacgctgct ggccgtgcgt ccggagagcg ccggcaggcc gtagttcagc
    11881 accgagcgcg cgacctgcgg ccaggcatcg agcggctgca cggattcgtg gttgccggcg
    11941 ttgaacagcc aggcgagatc acgcagcacg ctctcgcgca actggttcag cgacagcacg
    12001 cgctgctcgc gcgtttcctt gtgacgctcg ggcgcgtcgt cgatcagccg gtcgagcagc
    12061 gccggctgca ggcgttcctg cggggtcagt tcagccacgg tgagcctcag gcgctcgccg
    12121 gagcggccgg atcgaagtgc agcgtgcgca ggtcgtacag cgccacgtcg tcgccgtcgg
    12181 tcgcgaacac ccgctgcccg gcagcgagca cgaggccgtg ctcgttctcg cgccattcgg
    12241 tgcgccgtcc gagcagcagg ccgccgtcat cctcggcgtc cagccccggg tagcgcgccg
    12301 gcagcagcgc gaccgtctcg ccgccggtcg tgaaggtcag cgatgccggc atccagacca
    12361 catcgcgcag gtcggccggg gcctcctgct gcacgctgct caggcgttcg cagggaatcc
    12421 agtaatagcg gccgttgatg atcgcctcga tgaccggccc gagccggccg tcggtatcgg
    12481 ccagccacgc aaacggccgg ccgtcggcgc tgccgggatt ggccggtgcc cgctcgaagg
    12541 cttcggcgcg caggccggcc gcggcctgcg gatcgtcggc atcgcgcgcg agcgcctcga
    12601 tcagcagcgc gacccacggc gtcggctcgc cgaacagcag cggcgccctg cgaccggcga
    12661 agacctcctg ccggaagact tcgcagcgca gcgcctcgcg gtaggtctgc accatcggca
    12721 aggcctcggc atcgagttcg ccgaccacct gcagctggcc gagcgccctc ggccactggc
    12781 cgagcacgca gagcagctgg aacagcagca cgcgatggcg cgcaaccgcc ggctccttgc
    12841 gtacgcgggc ctgcagttcg gtcagtgcct cccggggatt gcccgactgc agggcggctt
    12901 cgacgctcac gacacacctc cacgccagcc gcccggtgca ggggcggctc acgtccggga
    12961 ttcggggaaa tccggaattc agacctgcgc cggagcgcag gccggcaggc gggcgggggc
    13021 cgcgagccgg cgttcgcaga cggaccggat tacggaacgg cgttggcctc gatgtcccac
    13081 ttgaagtcct tcgacgcctc accggcaccc ttgtcggtct gctcggtgta cgtgaaggtg
    13141 accttcgcga agttcagcga gacgttctcg gtcagccgat cctcgccacc gctgccaccg
    13201 gtgttgatcg acgtgaccat gaccttctcc atcttgatct tgatgtactc gaccggctcc
    13261 ttgccgccgg ccttgcgcac ggtcagcagc gcgtcgtcga tgtgctggcc ggtcgcgcag
    13321 gcgtgcatca gcgcatacga ggcgatgtcg acccacttgg tcaccgacat gtcgttgacg
    13381 ttgaccttgc cggcgccgcc accgccgccg gtgtggaagg tgccggactg cgacatgccc
    13441 cagctccagg cgaggacgtc gatttccttc ggatgcttct tgtccttcga ttcgcccttg
    13501 atgttcgcgg cgtccgcgaa tttcagaaag atgtcaacgg ccatgaatgt ctctcctgca
    13561 gtgcgtcaat gctggaattg ggacccggta cgtcgcccgg acgtcaggga ccgtttgcaa
    13621 aggtgcggcg accgcagtcg ccgcaccgcc agtcagccgc ccttctgcga gggcagcttg
    13681 gataccagtc gcaacgacac cgtcaatcct tcgagctggt agtgcggccg caggaagaac
    13741 ttcgacgtgt agtacccggg attgccttcg acttcctcga cgacgacctc ggcggcggcg
    13801 agcggcttct gggccttcgt ggtctcgctg gagcgcgagg gatcgccgtc cacgtagttc
    13861 atgatccatt tctgcagcca gatctgcatg tcctcgcgct ccttgaagga acccaccttg
    13921 tcgcggacca tgcacttcag gtagtgcgcg aagcggcagg tcgcgaacag gtacggcagc
    13981 cgggcggcga ggttggcatt cgaagtggca tcgggatcat cgtactcggc cggcttctgc
    14041 agcgactggg ctccgatgaa agctgcgaaa tcggtatttt tcttgtgcac gagcggcatg
    14101 aaaccgtttt ttgcgagctc ggcctcccgc cggtcggaaa tcgcgatttc ggtcgggcac
    14161 ttcatgtcga cgccgccgtc gtcggtcggg aaggaatgcg cgggcagccc ctcgaccgca
    14221 ccgccggatt cgatgccgcg gatgcgcgag caccagccgt agagcttgaa cgagcggttg
    14281 atgttgacgg ccatcgcgta ggcggagttg gcccacgtgt acttgttgct gtcggccccg
    14341 gtcgtgtcct cctcgaaatc aaattcatcg accggattgg tcttggcccc gtagggcatg
    14401 cgcgacagga agcgcggcat ggtcaggccg acgtagcgcg agtcctcgga ttcacgcagc
    14461 gacttccacg cggcatattc gggcgtctgg aagatcttgg tcaggtcgcg cgggttcgac
    14521 agctcctgcc aggaatccat ctgcatgacc gccggcgcga cggctgcgat gaacggggcg
    14581 tgcatcgagg cggaaacctt ggcgatctcg ccgagcagtt caacgtcggg cgccgaatgg
    14641 tcgaagtagt agtcgccgac caggcagccg tagggctcgc cgccgaactg gccgtattcc
    14701 tcctcgtaga gcttcttgaa gatcgggctc tggtcccagg ccgtgccctt gaacttcttc
    14761 agggtcttgt gcagatcctg cttggagatg ttcatgacgc ggatcttcag catctcgtcg
    14821 gtttcggtgt tgttgacgag gtgatgcagg ccgcgccagg ccgattcaag cttctggaaa
    14881 tcggcgtggt gcaggatgtg gttgatctgc tcggtcagct tggcatcgag cgcggcgatc
    14941 atcgcctcga tggtcttcag cgtgtcgccg gagacgatgc tcgacgacgc cagcgcctgc
    15001 tcggcgagcg tgcgcacggc ctcctcgacc gcttccttcg cgcggtcgct cttgggcttg
    15061 aattcctgca gcagcagcgc ctcgaaggcg ccgccctcgg tggtcaggcc cgcacccgcg
    15121 gcctgggctt cggcgttcag ttcggccatg gactggttac ctcggggtca ttcggactgc
    15181 ggtttgggcg cggacgcgag ggcctgcagc agggaggggt cctgcagcac cttgccgatc
    15241 agttcctcgg ccccggtctt gccgtccatg taggtcagca ggttggagag ctgggtgcgc
    15301 gcttcgagca gcttgttcag cgcatcgacc ttgcgggcga tcgcggccgg cgtgaagtcc
    15361 tccatgctct cgaaggtgat gtcgacgctc aggttgccgt cgccggtcag cgtgttcggc
    15421 accgcgaacg cgacgcgcgg cttcatcgat ttcatgcggc tgtcgaagtt gtcgacgtcg
    15481 atttccagaa acttgcgctc ggcgaccggt ccgagcggct cggcgggctt gccggacaga
    15541 tcggccatga cgcccatcac gaacggcagc tggaccttct tctgggcgcc gtagagctcg
    15601 acgtcgtact cgatctgcac gcgcggggcg cggttgcggg cgatgaattt ctgactgctg
    15661 tctgccacgg gcgggtctcc tcaggcgttg tgcgatcgcg aaccggcggc gcgtcggcac
    15721 cggctcgggg ggagcgggtc agtagccgtc cgagctggac gattcggcat ccggatcggg
    15781 gccgcgcagg acatcgatct gcgcgaggct gtcgggggcg atgttgcgca tgatgtcgac
    15841 gaaactcatc gtcaccagcc ggcgcgcgcg ctccatcagg atcggtaccg ggctcgaagg
    15901 ctcgtgtcga gtgtagtaat cgcagacggc ctccagagct ttcaatacgt catctcgcgt
    15961 actgatgcgc ccggcgacga aaccggaggc cccggccccg gccgccgcgc tgccgcccgc
    16021 ctcggccggc ccctcaccgg ccaccggcaa ggcctcggtc tcggccacgg cgcccaggcc
    16081 gcgctgggcc aggcgctcgt cgagcaactg cacgcagggc ttgagcgccg tgcgcagtgg
    16141 cgagaggtcc acggcgttgc cggaaccgac ctgctcggtc aggcggcgct cgattgccga
    16201 ggcgagatcg aaggccgcat gcgcgctgtc ggcggccgcc tgcagttcct cggcggcgac
    16261 ctcgcggaac acggcatcga aggtctcggt ggtcagcacc ggcgtgccgc tgctcgcgcc
    16321 ggacaggatc agccagtcgc gcagcgtcgc gacgccgaag ctgcgcgagc gcgccagcgc
    16381 ggcctggcgc agcaggccga gcacgtcgtc gctgtcggcg gcgacgaggc cggcgatgat
    16441 gttcacgcgc gtggtcggat cgttgtagtc atccggatcg agctgcggat gcacgtgctc
    16501 ccagtggtgc tccagcaggc cgtgcagata gcgcaggccg tcggcgaatc cgggcagtcc
    16561 ctcgacggcg agcagcgcgc gggtcagcac cagcgcgacg cgcaggtcgc gcgtgcgttc
    16621 gagcagttcc atcgccttgc cgcgcacggc ccgccagtcc ggcggttcgg ccggcacgat
    16681 ggtgctgccg atctgctgct cgggcttgcc ggtcgcgagc cgttcgaggg cctggaaatc
    16741 gggatcgtat tcgagatcac tgccaccggg ctcgtcgtcc gagacgggtt cgagccattt
    16801 ctccacatcc agcgccaccg gggcaccccc tgcgattgtc acccgatctg cgcaccggcc
    16861 ggcgccgcgc atcgtggcgt ccgccagtgc ggtcgctgcg tagcgtagcc ggcggccggc
    16921 ggactgccag ccggcagcga gcggcgaatt gtcagccgtg ccggcactgg cctacagtcg
    16981 gcgggctttt cgccccccgg cttgccgccc aggtttcacg accgatgccg ttgaccctcc
    17041 gtatcaccag ccagcacccg ctcctgcccg aggccgatac cgtgcgcagc ctcggcagcg
    17101 gcgacagcct gacgatcggc cgtgcgcccg gcaacgactg ggtgctgccg gacccggagc
    17161 tggtcatctc cggccgccac tgcaccgtgc acgaggagca ggggcgctac gtgctgaccg
    17221 acaccagcac caatggcgtc tatctcggcg gccagcgcgt cggtcgcggc aacagccggc
    17281 cgctgcaggc cggggacgtg atcgccatcg gcgagtacca gatcgccgtc gagccgggcg
    17341 ccggtgacgg cggacaagcc ccggccttcg gggccggcac ggcagcagcc gtggcgctgc
    17401 cggatgcctt cggccttgcc gcaccggcac cgctcgccga tccgttcggc ctgcacggca
    17461 cgccgggtgc gcaggacccg ttcgaactgc acggcaagcc ggccggcgag gatccgttcg
    17521 ggctgcgtgc gcccgcaacc ggtgatgatc cgttcgggct cggcaatgac ctgtccggcg
    17581 gtgacccctt cggactgatc ggcacgccgg gcaccagcgg cagcgcccgg ggcggcggcg
    17641 cgcccgcggc cgcggatccg ttcggtcttg ccggcggcgg tgaccgcaat ccggcgggcg
    17701 ccgatccgtt cgggctcggc ggcggcgccg gacggccgga tttcgcagcg ccggcaacgc
    17761 cggccggccc cgcgacggcc gaagccggcg gtgatccgtt cggcctgctg gcgccggcgc
    17821 cgctcacgtc tgccccggtt ccggatcacg tgccgtccga gcagcaggcc ttcgtgccgc
    17881 cggcgccggt cagtgacgat gactgggatg cactgtttcc ggccccggcc gctgccggcg
    17941 cagcgccggt gcggctggac attcccccgg ccgccaaaac ccccgatccg ctgttcgccg
    18001 gtctcggccc gctcgccgaa gaatccgcgc cgccggctgc cccgccacct gacgagaccc
    18061 ctgcggtgcc ggccttcacg cccgccccgg tctccgggcc cgtaccggcc ccggcgcccg
    18121 cacccatacc agtcccggca cccgcgccgg ccaccccggc cgctgcagcc ccggcggccg
    18181 aactgatggc ggccctgctg gaaggggccg gtctcgcgca tctgcagatt ccgcaagccc
    18241 gccaggccga tctgctgcgc gccctcggcg acagctaccg gcgcatggtg gagggcctga
    18301 tgaccgtgct gcgggcccgc tcgcacgtga agggcgagtt ccgcatgcag cagacctcga
    18361 tccggccgat cgagaacaat ccgctgaagt tcgcgccgaa cgtcgaggaa gcgatgacgc
    18421 tgctgctgac cctgcgcagc cagtcctacc tgtcgcccga acgcgccgtg gccgaggcct
    18481 tcgaggactt gcaggcgcat cagctggcga tgatggccgg catgcaggcg gcgctcggcc
    18541 acctgttccg gcgtttcgac cccgcgacgc tcgaagcccg cttcggcagc ggcggcctgc
    18601 tgcccggcag ccgcaaggcc cgctgctggg agcagttcac ggcgctgtat caggacatcg
    18661 cccgcgaggc cgaggacgat ttccaggaac tcttcgggcg cgagttcgtg cgcgcctacg
    18721 aagaacagat cgcccgcctg cgctcgcgct gagcccgcct gcacccggac cggcctgccg
    18781 accggttcgc gccgcctaca cttcgccgcg atcgccagtc gccaccggag acgtgccatg
    18841 aaccttcgcc acggcctgtc cgccctgggc ctgctcgtgc tgctcggcgc ctgcgccgcc
    18901 ccggccccgc cgccgccgac ccgcatcagc ggccagatcg aggcggcaac gccgctgaat
    18961 cccggccctg gcggcggttc gctgccgctg cgcgtgcggc tctacgaact gaagaacccg
    19021 ggcaccttcc agagcgccga cttcttctcg gtctgggaca acccgcaggc gaagctcggc
    19081 gccgacctgc tcggcaccga ggactacacg ctgcacgccg gtgcccgcca gcccttcgag
    19141 cgcacgctgg atccgcaggc gcgctacgtc ggcttcgtcg ccgcctaccg cgatctcaac
    19201 ggcgcggtct ggcgccagtc gctggcggtg ccggccaacc agaccacccg gctgcgcgtg
    19261 caggccggca cgaaaaccct gagcgtcggc gccgggcagt gacggcgctg ccgcttcctc
    19321 caaggcctct ttcacggagc atcgtctgca gatgagctgg cacagcaagg tcgtgtggtc
    19381 cgagggcatg ttcctgcgac cacagcactt ccagcagcag gagcgccatc tcgaccgggt
    19441 cctgcaaggc gtctgcggcg cgctcgaacc ctatgtctgg ggctttgcgg ggctcgcgct
    19501 cgatcgccag gcgctgacgc tcgggcgcat cgcgatcgcc tcggcgcgcg gcgtgctgcc
    19561 cgacggcacg ccgttctcga tcccggacga tgacgccgcg ccgccgccgc tggcggtgcc
    19621 cgatgatctg cgtgatgcgc gcgtggtgct ggcgctgccg ctgcgccgcc cgggcctgcg
    19681 cgacaccgcc cgcgatggcg aggatgccgc gatcacgcgc taccgcaccg agatcgccga
    19741 ggtgcgcgac aacaacgccg ggctcgaaag cctcgcgccg gtcgagctgg cgcgccacaa
    19801 cctgcgcctg ctgaccgatc gtcaggacct cggcgagtac gcctgcctcg gcatggtgca
    19861 cgtgatcgag aagaagaccg accagagcgt cgtgctcgac gagcgctacg tgccgccggt
    19921 gctcgatgcg ctgcagcagc cggtgctggc ggcgctggtg cgcgagatcg agggcctgct
    19981 gcaccagcgc gccgaggcgc tggccgggcg cgtcgttgcc tccggacgcg gtggcgtcgc
    20041 cgagattgcc gacttcctgc tgctgcaggc cgtcaatcgc gccgaaccgc tgttcacgca
    20101 tctggcgcgc gtgcgcgcgc tgcaccccga gccgctgtac cgcgagctgc tcgggctggc
    20161 cggcgaactg gcgaccttca cgcgcccgaa caagcgcccg cccgagttcc cggcctatga
    20221 ccacgacgat ctggccgcga ccttcgcggc ggtgatggcc gaaatccgcc aggcgctgtc
    20281 gatggtgctc gaacagaacg ccatccagat cccgctgcag gaacgcatcg ccggcgtgca
    20341 cgtcggcgtc atcaccgacc gcaacctgat cggcaaggcg agcttcgtgc tggcggtgcg
    20401 cgccgacgtg cccggcgaga tcctgcgtgc gcgcttcccg acccaggtca agatcggtcc
    20461 ggtcgagcac attcgcgacc tggtcacgcg ccagctgccc ggcatcggcc tcaacccgct
    20521 gccggtcgca ccgcgccaga ttccgtacca cgccggcctg acctacttcg aactcgatcc
    20581 ggcgagcgaa ctctggaagt cgctcgccag ttccggcggc ttcgcattcc acgtcgccgg
    20641 ccagttcccg gggctcgcac tgaccttctg ggcgatccgc agctgacctt ccggcaggag
    20701 catccgtcat ggtggatccg aacgatccgt tcgccggtct cggcggcgac accggcgacc
    20761 gcacgcaggt cctgaaaccc cggcccggtg gcgcgcgtgc gccgctgccg cagggcgcac
    20821 cgtcggcggc gaacgcggtg ccctcctacg caccgccccc gccaccggcc ccggcggcgc
    20881 cggtcgaggc gctgcgcttc ggcggcggca tcaatgcgct ggagcgcgcg gccgcgccgc
    20941 tgctcgcgct cgtgcacggc ctcaggaaca gcgtctcgca tcccgatccg ctcgggctgc
    21001 gccggcgcgt ggccgaggaa atccgcagct tcgaggcgcg cgcgcgcgcc gacggcgtgc
    21061 ggcaggagga aatcttcgcg gcgcgctacg tgctctgcac gctgctcgac gagaccgcga
    21121 tggccacgcc ctggggggcg agttcgggct ggagcgcgca gagcctgctc gtgcagttcc
    21181 acaacgaggc ctggggcggc gagaaggtct tccagctgct cgaaaaactg ctgcaggacc
    21241 cgcgcagcaa cctcaacctg ctcgaactcg tctacgtggt gctggcgctc ggtttcgagg
    21301 gcaagtatca cgtgctgccc gacggccgca accagctcga agccctgcgc gagcggctgt
    21361 accagaccct gcgcaccgtg cgcggcgagt tcgagcgcga gctgtcggtg cactggcgcg
    21421 gcgcgccggt caagcgcaat ccgctgagcc gctacgtgcc gctgtgggcg ctcggcgcgc
    21481 tggccgcgag cctgctgctc ggactgttcc tgatcctgcg cgccagcctc aatgccgagg
    21541 ccgaaccggt gtacgcgaac ctgcacggac tcggcgagaa cc
//
LOCUS       DAOQIE010000021        20617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1163_length_20617_cov_7.514785, whole genome shotgun sequence.
ACCESSION   DAOQIE010000021 DAOQIE010000000
VERSION     DAOQIE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 20617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20617
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_20617_cov_7.514785"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1056
                     /gene="nagZ"
                     /locus_tag="PK144_02555"
     CDS             1..1056
                     /gene="nagZ"
                     /locus_tag="PK144_02555"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNF64637.1"
                     /translation="MPLGPVMLGLPGLALGGADRELLRHPRVGGVILFARNYETPRQL
                     AELCAEIHALRSPRLLIGVDHEGGRVQRFREGFTVLPPMRRLGEVHDREPRRARAMAT
                     AAGWLMAAELRAADVDFSFAPVLDLDRGVSRVIGDRAFHSDPDVVATLAAALMHGLQS
                     AGMQAVGKHFPGHGHVEADSHHALPVDAREYADIEAEDLVPFGRLVRAGLAGVMPAHV
                     VYARVDERTAGYSPFWLREVLRSRLGFQGAIFSDDLDMAGAALAGGAPLDCARAALAA
                     GCDMVLACNDRRAALEIVHGLEDPDDPAAHLRLVRLHGRGQFEREALRASGQWQRAVR
                     LLTDPLAPLDEAAATAT"
     gene            1142..1507
                     /locus_tag="PK144_02560"
     CDS             1142..1507
                     /locus_tag="PK144_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNF64638.1"
                     /translation="MADKLLMILANTDPGSAVALHAVFAQATVAAAMEYDVEIVLTGR
                     TGRLATPGYAETVALAERGRTALDLIREAHEAGVRFKICTSARESADAAMIAEIAEIV
                     GGAYIISEAMDDATVTFTY"
     gene            1533..2084
                     /locus_tag="PK144_02565"
     CDS             1533..2084
                     /locus_tag="PK144_02565"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNF64639.1"
                     /translation="MQPTPEAVRAVLAEAEELVSPAAVQAALDRMAAAISARLADRDP
                     LVLVVLNGGVIPAGLLLPKLNFPLQIGYLHATRYRGATRGGGLHWVQKPSLPVQGRTV
                     LVIDDIFDEGHTLKEIVRELRAADAAEVLTAVLVDKLHARKVTDFTIDFTGTTVPDRY
                     VFGCGMDYHEYLRNLPGIYALRE"
     gene            2136..2879
                     /locus_tag="PK144_02570"
     CDS             2136..2879
                     /locus_tag="PK144_02570"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNF64640.1"
                     /translation="MSKLLAIIGGTGLTSLETLEIVRREMVSTPYGEPSGPVTHGVLA
                     GHPVVFLARHGYGHTIPPHQINYRANLWALKHIGVEHILAVAAVGGISARMGPGVLCV
                     PDQIIDYTYGRHHTLFEGDLSHVTHIDFTWPYDEALRQALIEGGRSAGIPLIEDGTYA
                     ATQGPRLETAAEIRRLERDGCDIVGMTGMPEAALARELGLRYACCAVVANWGAGKGEG
                     EITMEQIDQTLKAGMAQVRQLLRVVIPSV"
     gene            2918..3124
                     /locus_tag="PK144_02575"
     CDS             2918..3124
                     /locus_tag="PK144_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64641.1"
                     /translation="MNLPLPGWLPWLLIAIGVFDLGLAWMMRNALVKHPEAATPNLRR
                     VATFTQVSGLIAVAVGVGLLLFLR"
     gene            3176..3424
                     /locus_tag="PK144_02580"
     CDS             3176..3424
                     /locus_tag="PK144_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64642.1"
                     /translation="MNEMLALIAAQDWAALARLGALAWAAVFGTLAALWVWSELLLAL
                     GGGLRGGARTAVFALLFVLTAALLPLAALPAWRWLAGS"
     gene            complement(3543..4349)
                     /gene="cysE"
                     /locus_tag="PK144_02585"
     CDS             complement(3543..4349)
                     /gene="cysE"
                     /locus_tag="PK144_02585"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNF64643.1"
                     /translation="MAVQPLRDSAPATNDPLWETIRDEARITARQEPILASFLHASLL
                     NHRDLGAALSFTLAGKLDGATVPAMVVREVIEAAIEADPVLLDRVRADLLAVRERDPA
                     CPGYSVPLLYFKGFQAIQAYRVAHALWRDGREALALFLQSRIAEVFAVDIHPAARIGQ
                     GILLDHATGLVVGETAIIEDNVSMLQGVTLGGTGKESGDRHPKVRAGVLIGAGAKILG
                     NIEIGTGAKVGAGSVVLDPVPAHCTVVGVPARVVCQCREQEPSRSMDHHI"
     gene            4427..4840
                     /locus_tag="PK144_02590"
     CDS             4427..4840
                     /locus_tag="PK144_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64644.1"
                     /translation="MILVLLVVAIGLGLWWFFGQHNAASLDNLQASGFHADYTLDHNV
                     SPVALDSRTRRIAFLGLTRVRVYDYRQVISWRFDDYENPGGNDRSEPQIHRELVFRLD
                     DAEHPVVRLAGFDAEAARRWLATLQRELPQAGRGR"
     gene            5006..5344
                     /locus_tag="PK144_02595"
     CDS             5006..5344
                     /locus_tag="PK144_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNF64645.1"
                     /translation="MMSPKFLTAAAFAALLAATAARADELPLYTLTAKDGRFEPATIE
                     VTAGQKFRVEVVNAGSSAIEFESKPLRKEKVIAAGARATIDFQPLKAGSYRFFDEFHE
                     KTGQGEIVAR"
     gene            5378..6226
                     /locus_tag="PK144_02600"
     CDS             5378..6226
                     /locus_tag="PK144_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNF64646.1"
                     /translation="MGDVLFVVWRESVEALLVIGILYAWLRRQPEAGQGLRYLWLGVG
                     LGLGAAVLLGTMVLKAASLLDGDSGEIFQIAMVLFASALIVQMVCWMRRHGRTLKAEL
                     EQGMQRNLASHNWWGMVAVIVIAIAREGSETVIFLYGLGVQQQGGGLLSFVFSALVGL
                     GLAFATFALLQAGTRALSWRTFFRFSEAMLLLLAAALLTTGVEKLVSLGWLPGLVDPL
                     WDSSWLLDDSTTTGGLVAALTGYRAHPALTMLIAYVAYWGVVSWLLRAPALSGKAPSQ
                     VAAARG"
     gene            complement(6315..7301)
                     /locus_tag="PK144_02605"
     CDS             complement(6315..7301)
                     /locus_tag="PK144_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNF64647.1"
                     /translation="MFQAVYLDKPADRLEAGIRALPDDALPEGDVLVRVEWSTLNYKD
                     GLAITGKAPVVRKYPMVPGIDFAGTVEASQHPAWQPGDRVLLNGWGVGEGHWGGLAQK
                     ARVRGDWLLARPENLSARQCMAIGTAGYTAMLCVLALERHGVLADSGEVLVTGAAGGV
                     GSVAIQLLARRGYRVVASTGRIEAAGEYLRGLGAAEVIDRAQFSAPGKPLGRERWAGA
                     VDSVGSHTLANVCATTRYGGVVTACGLAGGMDLPATVAPFILRGVTLAGIDSVMAPLP
                     LRQAAWTALAAEIDTDKLDDMTRVIPLAEAIPAAAELIAGQVRGRLVVDVNA"
     gene            complement(7389..8015)
                     /locus_tag="PK144_02610"
     CDS             complement(7389..8015)
                     /locus_tag="PK144_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF64648.1"
                     /translation="MNPRPSSRPRGATRDELVHIGVRMLTEKGYGQTGLDEILRTAGV
                     PKGSFYHYFPSKEAYGLAVVEHYAQYFAKRLARAFDDDSLTPLDRLRRFVTEACAGMI
                     RHEFRRGCLVGNLGQELGAHNDAFRERLEAVLGHWQARVADCLRAAIRAGELPPETDA
                     EALAGFFWIAWEGAILRAKLTRSTAPLALCARVFFTQLLHAAPPSLPD"
     gene            complement(8040..>8238)
                     /locus_tag="PK144_02615"
     CDS             complement(8040..>8238)
                     /locus_tag="PK144_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64649.1"
                     /translation="RTVPRSAAGCRSGLPGKAFRPQAGLHACAAGPACTPEAARPSLP
                     RLFEIGIAATQKAAGSEAVIL"
     assembly_gap    8239..8338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8769..9404)
                     /locus_tag="PK144_02620"
     CDS             complement(8769..9404)
                     /locus_tag="PK144_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF64650.1"
                     /translation="MPTFVIAEDHALVREGLALRLSLEPGWEVIGETGDGSEVEGLVV
                     ERRPDVLILDLQLDGMQGLEVCTAVRRRLPDQLILVLTANPEADVARRALDAGADGYL
                     IKRGDGAELVHAIRTLLDGSGYISDDIGREVVSGGKVDTPVDALSPRERHVLALIAAG
                     HGSREIADQLSISVGTVRKHRENLMQKLDLHSIAEVTSFAIKNGLYTPPRG"
     gene            9548..11404
                     /locus_tag="PK144_02625"
     CDS             9548..11404
                     /locus_tag="PK144_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64651.1"
                     /translation="MGRPLPADGFSQAADAPAATVVEVDQVRYEAGLVELLYRQFGAI
                     FFGNALVLVFSVWLLRNYVPTLRLIGWALVVLAVNLTRRALVQAYERAPDRLSRPRRW
                     ARYYTVGAAMSGIVWGSVGLLFFNENNMTVTAYLCIVVAGMAGGSIATYGSWRPAYLM
                     FALPVALPFAARTLWEGGLVYTELGALCLFVLVLNLSFFSKIHATLCETVRLRLENQA
                     LVGELTIQKDNAEAATRVKARLLAAASHDLRQPVHSLGLFLGTLEELGRRPVLQPADV
                     GRIAVSMQRALGALSGLLGALLDLSRLDAGTVEPKPAPLALEDILDTLHSQFAGPARA
                     QGLRFASVPSRLWVETDRGLLLQVLSNLCTNALRYNRRGGGVLVGCRRIAGRRVTIQV
                     LDTGIGIAPEEQARVFGEFYQVAGAGRGREQGLGLGLAIVQRTARLLGGELTLRSVPG
                     RGSCFALTLPTRPAPAPAIVDAPHLEPLPASGPGRTVLIVDDDEQVLEALAMTLRVWG
                     HTVHAAADAASALLLARTHAAAIELVLADYRLGTETTGPVVVRELLAIFGRAVPVTIV
                     TGDTAPAVLTHILGDGHRLLHKPVDVGELRQLIEHLDAPLNPGLSARARAGG"
     gene            complement(11376..12587)
                     /gene="lplT"
                     /locus_tag="PK144_02630"
     CDS             complement(11376..12587)
                     /gene="lplT"
                     /locus_tag="PK144_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNF64652.1"
                     /translation="MTPFPSAAAAPGPLWSRGMAAVLFAQFFSAFGDNAVLFSALALL
                     RAEAYPDWAAPLLQQFFVAAYILLAPFAGPFADSLPKGRVMLAGNGLKLAGAAGLTLG
                     LNPFLAYGLIGVGAAAYSPAKYGILGELTTGDNLVKANGLMEASTIAAILAGAIAGGV
                     LADWSVGGALLVCTGVYALAAAANLLIPRLPPAHPIADARPGALLADFLAACRTIWQH
                     PDTRFSILGTSLFWGSGVTMRFLLVAWVPIALGLHDNATPAYLNAMVALGIVLGAGLA
                     GALVRLEQVERALPAGVGIGLAVALLALVNALVPAYALLAVVGACGGFFIVPLNALLQ
                     ERGRETVGAGHAIAIQNLGENGAMLAMLGLYTVALGAGTPVLWIAPLFGLGLSAAIAA
                     LWISRRRARAH"
     gene            complement(12701..12997)
                     /locus_tag="PK144_02635"
     CDS             complement(12701..12997)
                     /locus_tag="PK144_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64653.1"
                     /translation="MRPSARAAVLAALLAGLPASLPADPLAIDAGDDVGTLLNKYQGK
                     RVTLRLVSGEELTGTVRLVSARLVHISELASREFYDAAVVTGSIAAVIVRTRTQ"
     gene            complement(13094..13528)
                     /locus_tag="PK144_02640"
     CDS             complement(13094..13528)
                     /locus_tag="PK144_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF64654.1"
                     /translation="MPNRPVREVIANQTILTAPAAISVSEAAVRMKESRVGAVMVVET
                     DGALSGIFTERDALFRVLAAGLDPKTTKLAEVMTPHPQTIAPERPFGHALHMMYECGF
                     RHVPVIDAGKPIGMVSARDALGPELAVFQTEVQQREHIEEIL"
     gene            complement(13618..15750)
                     /gene="aas"
                     /locus_tag="PK144_02645"
     CDS             complement(13618..15750)
                     /gene="aas"
                     /locus_tag="PK144_02645"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HNF64655.1"
                     /translation="MLKSLLRALMRTLFRVEIRGRTDVFGHARTLIVANHESFLDGLL
                     LGLFLPVEPVFVVHTQVAVHPVFGRILRHIEHLAVDPANPLALKAIVRLVESGRPVVI
                     FPEGRITVTGSLMKVYDGAAFVAAKTGATVVPVRIAGAAQSFFGRLAGVYRLRLLPKI
                     RLHVLAPRVLPMPDRPKARDRRAVAGQNMRALLTEMLVLTRPGRTLFEAFLDARSTHG
                     ARTPIVEDLRLIEETYASMTKMALGLGLLVARHAGRGECVGLLLPNAAATVAAILGLS
                     LRGRIPAMLNYTAGVDGLRAACTAAEVRTVVTARAFLDKGKLDHLPGELGELRWLYLE
                     DLRAGMGLADKLRLLPRLLWPRLALDARVQPQDAAVVLFTSGSEGKPKGVVHSHDSLL
                     ANVAQVCAIADFTPRDKFMVALPLFHSFGLTAGALLPLVSGCKLFLYPSPLHYRVVPE
                     LTYDRNCTVLFGTSTFLGNYARFAHPYDFGRLRYVVAGAEKLDEGVRRTWIERFGIRI
                     LEGYGVTECAPVVACNVPMACRIGSVGHLVPGMQGRLQAVEGLEPGTGLLEVRGPNVM
                     LGYLRHERPGLIEPPAAAGETGWYSTGDIVSIDEDGYVFIRGRLKRFAKLAGEMVSLE
                     VVEQIARTAAPEARHAAATRADPARGEAIVLFTTDAALGREALLAAARTLGAPELAVP
                     RDIRALAEIPLLGTGKTDYVRLQTLAAS"
     gene            complement(15950..16270)
                     /locus_tag="PK144_02650"
     CDS             complement(15950..16270)
                     /locus_tag="PK144_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNF64656.1"
                     /translation="MILSTTPTLEGHRIREYRGIVSAEAILGANVVKDWFAGLTDFIG
                     GRSGTYEGVLRDAREEAFAELTRAAQRLGANAVVGIDIDYEVLGEQNGMMMVAVSGTA
                     VVID"
     gene            complement(16689..17042)
                     /locus_tag="PK144_02655"
     CDS             complement(16689..17042)
                     /locus_tag="PK144_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNF64657.1"
                     /translation="MNRTPRTENLTRLSSEEARQRIARGESESDWARVNAIPDDEIGA
                     DDPDALPMTDEELATAPILRRPGQRGLQKLPTKQRTTLRLSADVLAHFRADGAGWQTR
                     INDALKQWIAEHGER"
     assembly_gap    17096..17195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17196..17824)
                     /locus_tag="PK144_02660"
     CDS             complement(<17196..17824)
                     /locus_tag="PK144_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNF64658.1"
                     /translation="MLLHIPEVLPRDRLAGFRERLARADWLDGRRSAGHQALAVKHNQ
                     QLADDDPLARELAAQALAALERNPLFLTATLPARIFPPMINRYAGGGSYGNHVDNAVR
                     VLPDGTRVRTDVSATLFLSEPEDYDGGELTIDDTYGTHTVKLPAGDLVIYPSTSLHRV
                     TPVTRGARIACVFWVQSLIRDDAQRTLLLDLDLAIQRLNRSGTEPDVAVA"
     gene            complement(17928..20108)
                     /locus_tag="PK144_02665"
     CDS             complement(17928..20108)
                     /locus_tag="PK144_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNF64659.1"
                     /translation="MPIHAPHPLARPSAGIRSTARTPLQTGCLLGALGAALPGSVCAA
                     DGDSAALPEVTVTAPQDQGYLRDGTGLTRLPGPLRDIPQSITVVPQQIIEEQGATTLR
                     EALRNVSGISFNGGEGGNSGDNINLRGFTARNDLFLDGVRDSGSYTRDIFNIEAVEVL
                     KGPSSMVFGRGTTGGAINQTSKRPWLESAHTVGASLGTLDLRRLTVDFNEPLTSTAAF
                     RLNGMWQQSGATPEDVQFDRYGIAPSLTLGLGTDTEVNFSYFRQKEDNVPYYGVPYRN
                     FKPVDVDKKNFYGLADHDFENTTVDIGSITVDHRFNDSLSFRNSTRYARYDRSLAPSA
                     PRIAGNPPDGTPYELISVNRSRPYRDGTDTTLANQTDITAKFTTGSFEHKLVGGVEIG
                     RETTTTTRYQVTGAPQTNLAHPDPDTPAPLMRVVGTSSYTDASANTFGVYAIDEIALT
                     PEWKVVGGLRWDRFAASSLAETFAPATGTQLTHDDRKRTDTMTSGRIGVIWQPSPTQS
                     YYAAFGTSFNPTAETLTISSNNAGLDPEENHNFEIGAKLDFLDGALSVRTALFRTEKI
                     NARTLDPDQLNVNVLDGRWVTDGIELDVIGRITPQWSVFAGLAFMDPEITESNNPAEV
                     GKRPANAPRHTLSVWTTYDLGGGWEIGGGVQGAGKRYGSNTNTNYVPGYTRWDAEVAY
                     KQPDFDVRLNVVNLTDTQWYEAVYTGHAIPGTGRAATLSVDYRF"
     gene            complement(20347..>20617)
                     /locus_tag="PK144_02670"
     CDS             complement(20347..>20617)
                     /locus_tag="PK144_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64660.1"
                     /translation="AALLRADRLGGRLRLSGAGFLAELDERAIAQTWVVHRPEGLRSR
                     ARSTLELYAADGRLLAALGAPPRPTDGSECAVWRALLLGLPGLIH"
BASE COUNT         2888 a         6991 c         7410 g         3128 t
ORIGIN      
        1 atgccgctcg gtcccgtcat gctcggtctg cccggcctcg cgctgggcgg agccgaccgc
       61 gaactgctgc gccatccgcg cgtcggcggc gtgatcctgt tcgcgcgcaa ctacgaaacc
      121 ccgcgccagc tcgccgaact ctgcgccgaa attcacgcgc tgcgctcgcc gcggctgctg
      181 atcggcgtcg atcacgaggg cgggcgcgtg cagcgcttcc gcgagggctt cacggtgctg
      241 ccgccgatgc gccgcctcgg cgaggtccac gaccgcgagc cgcgccgggc gcgggcgatg
      301 gcgacggcgg ccggctggct gatggcggcc gagctgcggg ccgccgatgt cgatttcagc
      361 ttcgcgccgg tgctcgatct cgatcgcggc gtcagtcgcg tcatcggtga ccgcgccttc
      421 cattccgacc cggatgtggt cgcgacgctg gccgcggcgc tgatgcacgg cctgcagtcg
      481 gccggcatgc aggcggtcgg caagcatttc cccgggcacg gccacgtcga ggctgattcg
      541 caccacgcgc tgccggtcga tgcgcgcgag tacgccgaca tcgaggccga ggatctcgtg
      601 cctttcggcc ggctggtgcg cgccggcctg gccggcgtga tgccggcgca cgtggtctat
      661 gcgcgcgtcg atgagcgcac cgccggctac tcgccgttct ggttgcgcga ggtgctgcgc
      721 agccggctcg gcttccaggg cgcgatcttc agcgatgatc tcgacatggc cggcgcggcg
      781 ctggccggcg gggcgccgct cgattgcgcg cgcgccgcgc tcgcggccgg ctgcgacatg
      841 gtgcttgcct gcaacgaccg gcgcgcggcg ctggagatcg tgcacggcct cgaagacccg
      901 gacgatccgg ccgcgcacct gcgtctggtg cgtctgcacg gccgcggcca gttcgagcgc
      961 gaggcgctgc gggcttccgg gcaatggcag cgcgccgtgc gcctgctcac cgatccgctg
     1021 gcgccgctcg acgaagccgc ggcgaccgcg acctgaacac cggctgcgtt ggccctgccg
     1081 gcgacgcatc cctataatcc cgcgccctgc cggcggggac cgccggagcg gagatgcatg
     1141 catggccgac aaactgttga tgatccttgc caataccgat cccggcagtg ccgtggcgct
     1201 gcacgcggtg tttgcccagg cgaccgtcgc ggccgcgatg gaatacgacg tcgagatcgt
     1261 gctgaccggg cgcaccggcc ggctggcgac gccggggtat gccgagacgg tcgcgctcgc
     1321 cgagcgcggg cgcaccgcgc tcgacctgat ccgcgaggcc cacgaggccg gcgtgcggtt
     1381 caagatctgc accagcgccc gcgagagcgc cgatgcggcg atgatcgccg agatcgccga
     1441 gatcgtcggt ggcgcctaca tcatcagtga ggccatggac gacgccacgg tgacgttcac
     1501 gtactgaaag ctccgcccgg agaaccgatc cgatgcagcc tacgcccgaa gccgtccgtg
     1561 ccgtgctcgc cgaagccgag gaactcgtgt cgccggcggc cgtgcaggcg gcgctcgatc
     1621 gcatggccgc ggccatcagc gcgcggctcg ccgatcgcga tccgctggtg ctggtggtgc
     1681 tcaacggcgg cgtgatcccg gccggcctgc tgctgccgaa gctgaacttc ccgctgcaga
     1741 tcggctatct gcacgcgacg cgctaccgcg gtgccacgcg cggcggcggc ctgcactggg
     1801 tgcagaagcc gagcctgccg gtgcaggggc gcacggtgct ggtcatcgac gacatcttcg
     1861 acgaaggcca cacgctgaag gagatcgtgc gcgaactgcg cgccgccgat gccgccgagg
     1921 tgctgaccgc ggtgctggtc gacaagctgc atgcgcgcaa ggtcacggac ttcaccatcg
     1981 acttcaccgg cacgacggtg ccggatcgct acgtgttcgg ctgcggcatg gactaccacg
     2041 agtacctgcg caacctcccc ggcatctacg cgctgcgcga atgacgccac gccctgcccg
     2101 gcccctttcc ctgtccgccc cacggagctg aaccgatgtc caagctgctc gcgatcatcg
     2161 gcgggaccgg cctgacctcg cttgaaaccc tggagatcgt gcgccgcgag atggtctcga
     2221 cgccgtacgg cgagccgtcc gggccggtca cgcacggcgt gctcgccggt cacccggtgg
     2281 tgttcctcgc ccgccacggc tacggccaca ccatcccgcc gcaccagatc aactaccgcg
     2341 ccaatctctg ggcgctgaag cacatcggcg tcgagcacat cctcgcggtc gccgcggtcg
     2401 gcggcatcag tgcgcgcatg ggaccgggcg tgctgtgcgt gcccgaccag atcatcgact
     2461 acacgtatgg ccgccatcac acgctgttcg agggcgacct cagccacgtc acgcacatcg
     2521 acttcacgtg gccgtacgac gaggccctgc gccaggcgct gatcgaaggc ggacgcagcg
     2581 ccggcatccc gctgatcgag gacggcacct acgccgccac gcaggggccg cgtctggaaa
     2641 ccgcggccga aatccgccgg ctcgaacgcg acggctgcga catcgtcggc atgaccggca
     2701 tgcccgaggc cgcgctcgcc cgcgaactcg gcctgcgcta cgcctgctgc gcggtggtcg
     2761 ccaactgggg cgccggcaag ggcgagggtg agatcaccat ggagcagatc gaccagaccc
     2821 tgaaggccgg catggcgcag gtgcgtcagc tgctgcgcgt cgtgatcccg agcgtctgaa
     2881 cccgcgtttc cgaaccagac ccggagagac tccccgcatg aacctgcccc tgcccggatg
     2941 gctgccctgg ctgctgatcg cgatcggcgt gtttgatctc ggtcttgcct ggatgatgcg
     3001 caatgcgctg gtgaagcacc ccgaggccgc gaccccgaac ctgcgccgcg tcgcgacctt
     3061 cacgcaggtc tccggcctga tcgcggtggc ggtcggtgtc ggcctgctgc tgttcctgcg
     3121 ctgacggcgc ctgcgctctg ccgatttcct tcccttcccg atccggagat gcccgatgaa
     3181 cgagatgctg gcgctgatcg ccgcacagga ctgggccgca ctcgcgcggc tcggcgctct
     3241 ggcctgggcc gcggtgttcg gcacgctcgc cgcgctgtgg gtctggtccg aactgctgct
     3301 cgcgctgggc ggcggcctgc gcggcggtgc gcgcacggcg gtgttcgcgc tgctgttcgt
     3361 gctcacggcc gcgctgctgc cgctcgccgc cctgccggcc tggcgctggc tggccgggag
     3421 ctgaatgcgc tgcttttcct gaagcagctt ccctgatgcg gcgaaggccg tccggtcgca
     3481 caggaccgga cggccttcgt gatttttccg ggcagcggcc ggcggcgggg ccggggcggc
     3541 cctcagatgt ggtgatccat cgagcgcgac ggctcctgct cgcggcactg gcagaccacg
     3601 cgcgccggca cgccgacgac ggtgcagtgc gccggcaccg ggtccagcac gacgctgccg
     3661 gcgccgacct tggcgccggt gccgatctcg atgttgccga gaatcttggc gccggcgccg
     3721 atcagcacgc cggcgcgcac cttcggatgg cggtcgccgc tttccttgcc ggtgccgccg
     3781 agcgtcacgc cctgcagcat cgagacgttg tcctcgatga tggcggtctc gccgaccacg
     3841 aggccggtgg cgtgatcgag caggatgccc tgaccgatgc gtgccgccgg atggatgtcg
     3901 accgcgaaca cctcggcgat gcggctttgc aggaacagcg ccagtgcctc gcggccgtcg
     3961 cgccacagcg cgtgcgcgac gcggtaggcc tggatggcct ggaaaccctt gaagtacaag
     4021 agcggcaccg aatagcccgg gcaggccggg tcacgctcgc gcaccgcgag caggtcggcg
     4081 cgcacgcgat cgagcagcac cggatcggcc tcgatcgcag cctcgatcac ctcgcgcacg
     4141 accatcgccg gcaccgtcgc accgtcgagc ttgccggcca gcgtgaatga cagtgccgcg
     4201 ccgagatcgc ggtgattcag cagcgaggca tgcaggaagc tcgcgaggat cggttcctga
     4261 cgggcggtga tgcgtgcctc gtcacggatg gtttcccaga gcgggtcgtt cgtggcgggc
     4321 gcggagtcgc gcaggggctg gacggccatc ggaatacctc gccggttcgg aagggccgcc
     4381 gtgtcagaat cgggcttccc gatcctgagc cgcggaggca actggaatga tcctggtcct
     4441 gctcgtcgtg gccatcggcc tcggcctctg gtggttcttc ggccagcaca acgcggcgtc
     4501 cctggacaat ctgcaggcga gcggctttca cgccgattat acgctcgacc acaacgtcag
     4561 cccggtggcc ctcgacagcc gcacacgccg catcgccttc ctcggcctga cgcgcgtgcg
     4621 cgtctacgac taccgccagg tcatcagctg gcgtttcgac gattacgaga accccggcgg
     4681 caatgatcgt tccgagccgc agatccaccg cgaactggtg ttccggctcg acgatgccga
     4741 gcacccggtg gtccggctcg ccggcttcga cgccgaggcg gcgcgccgct ggctggcgac
     4801 gctgcagcgc gaactgccgc aggccgggcg cgggcgctga ggagctggcg cgaatcccgc
     4861 agaaaattcc gggttgcgcc aaatgagaat tgtttttatc tggacgggtc ggcatcatcg
     4921 ctgcaagcgt ccgcggacgc agggttccgt tccggttccc gtaccgattc ctgcccccga
     4981 tcccgtctgc accccggagt ctgccgtgat gagcccgaag ttcctgaccg ccgctgcctt
     5041 cgccgcgctg ctcgccgcta ccgccgcgcg cgccgatgaa ctgccgctgt acacgctgac
     5101 cgcgaaggac ggtcgcttcg agccggcgac gatcgaggtc acggccggcc agaaattccg
     5161 cgtcgaggtc gtcaatgccg gcagctcggc gatcgagttc gagagcaagc cgctgcgcaa
     5221 ggagaaggtc atcgccgccg gcgcgcgcgc gacgatcgac ttccagccgc tgaaggccgg
     5281 cagctacagg ttcttcgacg agttccacga gaagaccggc cagggcgaga tcgtcgcgcg
     5341 ctgagcgcgg ccgggagcag tcatcatgat gggcatcatg ggcgacgtgt tgttcgtggt
     5401 ctggcgcgag agcgtcgagg cgctgctggt catcggcatc ctgtatgcgt ggctgcgccg
     5461 tcagcccgag gccggtcagg gcctgcgcta tctgtggctc ggcgtcggac tcgggctcgg
     5521 ggcggccgtg ctgctcggca cgatggtgct gaaggccgcg agcctgctcg atggcgacag
     5581 cggcgagatc ttccagatcg cgatggtgct gttcgcatcc gcgctgatcg tgcagatggt
     5641 ctgctggatg cgccgccacg gccgcacgct gaaggccgaa ctcgaacagg gcatgcagcg
     5701 caacctcgcc agccacaact ggtggggcat ggtcgccgtc atcgtcatcg ccatcgcccg
     5761 cgagggcagc gagacggtga tcttcctgta cggcctcggc gtgcagcagc agggcggcgg
     5821 gctgctgagc ttcgtgttct cggcgctggt cggcctcggg ctggcgttcg cgaccttcgc
     5881 actgctgcag gccggcacgc gcgcgctgtc gtggcgcacg ttcttccgct tcagcgaggc
     5941 gatgctgctg ctgctcgccg cggcgctgct gaccaccggc gtcgagaagc tcgtctcgct
     6001 cggctggctg ccgggtctcg tcgatccgct gtgggacagc agctggctgc tcgatgactc
     6061 gaccacgacc ggcggtctgg tcgcggcgct gaccggctac cgtgcgcatc cggcgctgac
     6121 gatgctgatc gcctacgtcg cgtactgggg cgtcgtcagc tggctgctgc gcgccccggc
     6181 gctcagcggc aaggcaccgt cgcaggtcgc cgccgcacgc ggctgaaccc ggcaccggct
     6241 ccggcccgcg ccccgctgcg cttgctgctg cagcggggcg cggcgtttcc ggcgccggct
     6301 ccggcatggc cggatcaggc attgacgtcg acgaccagcc ggccgcgcac ctgcccggcg
     6361 atcagctcgg ccgcggccgg gatcgcctcg gcgagcggga tcacgcgggt catgtcatcg
     6421 agcttgtcgg tgtcgatctc ggccgcgagc gccgtccagg ccgcctggcg cagcggcagc
     6481 ggcgccatga cgctgtcgat gccggcgagg gtcacgccgc gcaggatgaa cggcgcgacc
     6541 gtcgccggca ggtccatgcc gccggccagc ccgcaggcgg tgacgacgcc gccgtagcgc
     6601 gtcgtcgcgc agacgttggc cagcgtgtgg ctgccgaccg agtcgacggc tccggcccag
     6661 cgctcgcgcc cgagcggctt gccgggggcc gagaactgcg cgcggtcgat gacttcggcc
     6721 gcaccgagcc cgcgcagata ctcgccggcg gcctcgatcc ggccggtcga ggcgacgacg
     6781 cggtagccgc gacgcgcgag cagctggatc gcgacgctgc cgacgccgcc ggcggcgccg
     6841 gtcaccagca cctcgccgga atcggcgagc acgccgtgac gctccagcgc cagcacgcac
     6901 agcatcgccg tgtagccggc cgtgccgatc gccatgcact ggcgcgccga gaggttctcc
     6961 ggtcgcgcca gcagccagtc cccgcgcacg cgcgccttct gcgcgagccc gccccagtgg
     7021 ccttcgccga cgccccagcc gttgagcagc acccggtcgc cgggctgcca ggccggatgc
     7081 tggctggcct cgacggtgcc ggcgaagtcg atgccgggca ccatcgggta cttgcgcacg
     7141 accggcgcct tgccggtgat cgcgaggccg tccttgtagt tgagcgtcga ccattcgacg
     7201 cgcacgagca cgtcgccttc cggcagcgcg tcgtcgggca gggcacggat gccggcttcg
     7261 agccggtcgg cgggcttgtc gagatatacg gcctggaaca tcgggactct cctcggggca
     7321 tgagaccggt tcctaacaga ccggtcggtc ggtgatcggg caaatgaaag cccgacggga
     7381 atgcggattc agtccggcag cgacggtggc gcggcgtgca gcagctgcgt gaagaacacc
     7441 cgggcgcaga gggcgagcgg ggcggtcgag cgcgtcagct tggcgcgcag gatcgcgccc
     7501 tcccaggcaa tccagaagaa gccggccagc gcctcggcgt cggtttccgg cggcagctcg
     7561 ccggcgcgga tcgccgcgcg caggcagtcg gcgacgcgcg cctgccagtg gccgagcacg
     7621 gcctccagcc gctcgcggaa ggcgtcgttg tgcgcaccga gttcctgacc gagattgccg
     7681 accaggcagc cgcggcggaa ttcgtgccgg atcatgccgg cgcaggcttc ggtcacgaag
     7741 cgccgcagcc ggtcgagcgg ggtgagcgag tcgtcatcga aggcgcgcgc gaggcgcttc
     7801 gcgaagtact gcgcgtagtg ctcgacgacg gcgagtccgt acgcctcctt gctcggaaag
     7861 tagtgataga acgagccctt gggcacgccg gcggtgcgca ggatctcgtc gagaccggtc
     7921 tggccgtagc ccttctcggt cagcatgcgc acaccgatgt gcacgagttc gtcgcgcgtg
     7981 gcgccgcgcg ggcgggaaga tgggcgtggg ttcatggggc tggatattag accggtcgtc
     8041 tagagaatga cagcctcact tccggccgcc ttctgcgtgg cagcgatacc gatttcgaag
     8101 aggcggggga gggacggccg tgctgcctcc ggcgtgcagg ccggtccggc agcgcaggcg
     8161 tgcaggccgg cctgcggacg gaaggctttg ccgggaagac cggaccggca gcccgccgcg
     8221 gaacgcggca cggtgcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     8341 gttgcgggcg atctgcgctg ccggccggag acggcggaat ggttgcgccc aaccgttccg
     8401 ccgccccggc catgacggcc gccgtaccgg aagcgcaggt gactgacccg tggcctggcg
     8461 ctgtccccgc gacaacgccg gccactcacc cgtccgatgc cggcctccgt gccgatggca
     8521 acccgtatca gcagccccgc ttcagcgacc ggccggcgtc cagcccgccg gtgcccgctg
     8581 gtttccgtac cggcgacccc gggttgcggt gcacttccct gcaccttgcg ggttgatgcg
     8641 gtgcgctccg gcaggggccg aagcacgggg ggagtgtcga tgccgcagga cggcggaaca
     8701 atccggcaaa cgtcgtatcc ggtccgcctg cgtcgcaggc gtgaggactc ggggaggatt
     8761 cagggaattc agccgcgcgg cggcgtgtac aggccgttct tgatcgcgaa gctcgtgacc
     8821 tcggcgatgc tgtgcaggtc gagcttctgc atcaggttct cgcggtgctt gcgcacggtg
     8881 ccgacgctga tcgacagctg gtcggcgatc tcgcggctgc cgtgcccggc ggcgatcagc
     8941 gccagcacgt ggcgttcgcg cgggctcagt gcatcgaccg gggtatcgac cttgccgccg
     9001 ctgacgacct cgcggccgat gtcgtcgctg atgtaaccgc tgccatcgag cagcgtgcgg
     9061 atcgcatgca ccagttcggc gccgtcgccg cgcttgatca ggtagccgtc ggcaccggca
     9121 tcgagcgcac ggcgtgcgac atcggcctcc gggttggcag tcagcaccag gatcagctga
     9181 tcgggcaggc gccggcgcac cgcggtgcag acctccaggc cctgcatgcc gtcgagctgc
     9241 aggtcgagga tcagcacatc gggccttcgc tcgacgacca gaccctcgac ttcgctgccg
     9301 tcgccggtct cgccgatgac ctcccacccc ggctccagcg acaggcgcag ggccaatcct
     9361 tcccggacca gcgcatggtc ctcggcgatc acgaacgtcg gcatttccgt ttcctctgct
     9421 gctattacgt caaatatcgt atcccaccca cggcaggcgc gcaggcgtgt gccggagacc
     9481 gcgagtcgca gccccgccgg gcgcgcggcc tgcggttcgg tatccggatg cagcacagga
     9541 tgaagcaatg ggcagaccac ttccggcaga cggattttcc caggccgcgg atgcaccggc
     9601 agccacggtc gtcgaggtcg atcaggtccg ctacgaggcc gggctggtcg agctgctgta
     9661 ccggcagttc ggggcgatct tcttcggcaa tgcgctggtg ctggtgtttt ccgtctggct
     9721 gctgcgcaac tacgtgccga cgctgcggct gatcggctgg gcactggtgg tgctcgccgt
     9781 caacctgacg cgccgggcgc tggtgcaggc ctacgagcgg gcaccggatc gcctgagccg
     9841 gccgcgacgc tgggcgcgct actacaccgt cggcgcggca atgtccggca tcgtctgggg
     9901 ctcggtcggc ctgctgttct tcaacgagaa caacatgacc gtcacggcgt atctctgcat
     9961 cgtcgtcgcc ggcatggccg gcggttcgat cgcgacctac ggctcgtggc ggccggccta
    10021 cctgatgttc gcgttgccgg tggcgctgcc gtttgcggcg cgcacgctgt gggagggcgg
    10081 gctggtctac accgagcttg gggcgctgtg cctgttcgtg ctggtgctga acctgtcgtt
    10141 cttctcgaag atccacgcga cgctgtgcga gacggtgcgc ctgcgcctcg aaaaccaggc
    10201 gctggtcggg gagctgacca tccagaagga caatgccgag gccgcgacgc gcgtcaaggc
    10261 acggctgctg gcggcggcga gtcacgacct gcgtcagccc gtgcattcgc tcggcctctt
    10321 cctcggcacg ctggaggaac tcggccggcg gccggtactg cagccggccg atgtcggacg
    10381 catcgcggtc agcatgcagc gcgcgctcgg tgcgctgtcc ggcctgctcg gggccctgct
    10441 cgacctgtca cggctcgatg ccggcacggt cgagccgaag ccggcgccgc tcgcccttga
    10501 ggacatcctt gacacgctgc acagccagtt cgccggtccg gcacgtgcgc agggcctgcg
    10561 cttcgccagc gtgccgagcc gcctctgggt cgagaccgat cgcggcctgc tgctgcaggt
    10621 gctgtcgaac ctctgcacca atgcgctgcg ctacaaccgc cgcggcggag gcgtgctggt
    10681 cggctgccgg cggatcgccg gccggcgcgt cacgatccag gtcctggaca ccggcatcgg
    10741 catcgccccc gaggaacagg cgcgcgtgtt cggcgagttc tatcaggtcg ccggggccgg
    10801 gcgcgggcgc gagcaggggc tcgggctggg gctggcgatc gtgcagcgca cggcgcggct
    10861 gctcggcggc gaactgacgc tgcgctccgt gcccggacgc ggttcctgct tcgcgctgac
    10921 gctgccgacg cgcccggcgc cggcgccggc gatcgtcgat gccccgcatc tggagccgct
    10981 gccggcgagc gggccggggc gcacggtgct gatcgtcgat gacgatgagc aggtgctcga
    11041 agcgctggcg atgacgctgc gcgtctgggg ccacaccgtg catgccgccg ccgatgccgc
    11101 gagcgcgctg ctgctcgcgc gcacgcacgc ggcggcgatc gagctggtgc tcgccgacta
    11161 ccgcctcggc accgagacca ccgggccggt ggtcgtgcgc gagctgctcg cgatcttcgg
    11221 ccgggcggtg ccggtgacca tcgtcaccgg tgacacggca ccggcggtgc tgacgcacat
    11281 cctcggcgac ggccaccggc tgctgcacaa gccggtcgat gtcggcgagc tgcgccagct
    11341 catcgagcat ctcgacgcgc cgctgaaccc cgggctcagt gcgcgcgcgc gcgccggcgg
    11401 ctgatccaga gcgccgcgat cgccgccgac aggccgagtc cgaacagcgg cgcgatccac
    11461 agcaccggcg tgccggcccc gagcgcgacc gtgtacagcc cgagcatcgc cagcatcgcg
    11521 ccgttctcgc cgaggttctg gatcgcgatc gcgtggccgg ccccgaccgt ctcgcgcccg
    11581 cgctcctgca gcagcgcgtt cagcggcacg atgaaaaatc cgccgcaggc cccgaccacg
    11641 gcgagcagcg cataggccgg caccagcgcg ttcacgagcg cgagcagcgc caccgccagg
    11701 ccgatgccga cgccggccgg cagcgcacgt tcgacctgtt cgagccgcac cagcgcgccg
    11761 gcgagccccg ccccgagcac gatgccgagc gcgaccatcg cgttcagata ggccggcgtg
    11821 gcgttgtcgt gcaggccgag cgcgatcggc acccaggcca ccagcaggaa gcgcatcgtc
    11881 acgcccgagc cccagaacag gctggtgccg aggatcgaga agcgcgtgtc ggggtgctgc
    11941 cagatcgtgc ggcaggcggc gaggaaatcc gcgagcagcg cgccgggccg ggcatcggcg
    12001 atcgggtgtg ccggcggcag gcgcgggatc agcaggttgg cggcggcggc gagtgcgtag
    12061 acgccggtgc agaccagcag cgccccgccg acgctccagt cagcgagcac gccgccggcg
    12121 atggccccgg cgaggatcgc ggcgatggtc gaggcttcca tcaggccgtt ggccttgacc
    12181 agattgtcgc cggtggtcag ctcgccgagg atgccgtact tcgccggtga ataggccgcg
    12241 gcgccgacgc cgatcagtcc gtaggcgagg aagggattca gccccagcgt cagcccggcg
    12301 gccccggcga gcttcaggcc gttgccggcc agcatcaccc ggcccttggg caggctgtcg
    12361 gcgaacggcc cggcgaaggg tgcgagcagg atgtaggcgg cgacgaagaa ctgctgcagc
    12421 agcggcgcgg cccagtccgg ataggcctcg gcgcgcagca gcgccagcgc cgagaacagc
    12481 accgcgttgt caccgaaggc cgagaagaac tgcgcgaaca gcacggcggc catgccgcgc
    12541 gaccacagcg ggccgggtgc ggcggccgca gacggaaacg gagtcattcg gggggcatcc
    12601 gggcggcggt tgggtgccgg cagtgtagcc ggcgccggtc gccggccggc cgggaggcgc
    12661 gggcggaggc agccggcgcg ccggtgctgc ctgcccgaac ttactgcgtg cgcgtgcgca
    12721 cgatcaccgc ggcgatgctg ccggtcacga ccgccgcgtc gtagaactcg cggctggcga
    12781 gttcgctgat gtgcacgagc cgggcactga ccagacgcac cgtgccggtc agttcctcac
    12841 cggaaaccag gcgcagcgtc acgcgcttgc cctggtactt gttcagcagc gtgccgacat
    12901 catcaccggc gtcgatggcg agcggatcgg cgggcaggct ggcaggcagg ccggccagca
    12961 gcgcggcaag cacggcggcg cgggcggaag gacgcatggc gggtctcctc gcggcggaca
    13021 tgaaaaggcc gtgcaccgct cgcggacggc acacggccgg atcggtagcc cggtcggggc
    13081 tggcgcggtg ggcttacagg atttcctcga tgtgctcgcg ctgctgcact tcggtctgga
    13141 acacggccag ctcggggccg agcgcatcgc gcgccgagac catgccgatc ggcttgccgg
    13201 catcgatgac cggcacgtgg cggaagccgc actcgtacat catgtgcagg gcatggccga
    13261 acgggcgttc gggggcgatc gtctgcggat gcggggtcat gacctcggcg agcttcgtcg
    13321 tcttcggatc aaggccggcg gcgagcacgc ggaacagggc atcgcgctcg gtgaagatgc
    13381 cgctgagggc gccgtcggtt tcgacgacca tcacggcgcc gacgcggctt tccttcatcc
    13441 tcacggcggc ctcgctgacg ctgatggcgg ccggagccgt caggatcgtc tggttggcga
    13501 tgacttcgcg tacggggcgg ttgggcatgg caggcacctc tcggcggggt ttgtctcaac
    13561 cagtggtttc tagtcgatgt gcagcacagt cgtggtctgc tgaagctaca aacgttttca
    13621 cgatgcggct agcgtttgca gccggacgta gtccgtcttg ccggtgccga gcaagggtat
    13681 ctccgccagc gcacggatgt cgcgcggcac ggccagttcc ggggcaccga gcgtgcgcgc
    13741 cgccgcgagc agcgcctcgc gcccgagcgc ggcatcggtc gtgaacagca cgatggcctc
    13801 gccgcgcgca ggatcggcgc gcgtggccgc cgcgtgccgg gcttcgggcg cggctgtgcg
    13861 cgcgatctgc tcgacgactt cgagcgacac catctcgccg gcgagcttgg cgaagcgctt
    13921 cagccgtccg cggatgaaca cgtagccatc ctcgtcgatc gagacgatgt cgccggtcga
    13981 ataccagccc gtttccccgg ccgccgccgg cggctcgatc aggcccgggc gttcgtggcg
    14041 caggtagccg agcatgacgt tcggcccgcg cacttcgagc aggccggtgc ccggctccag
    14101 gccctcgacc gcctgcagcc gcccctgcat gcccggcacc agatgcccga ccgaaccgat
    14161 gcggcaggcc atcggcacgt tgcaggcgac caccggcgcg cactcggtga cgccgtagcc
    14221 ttcgaggatg cggatgccga agcgctcgat ccaggtgcgg cgcacgccct cgtcgagctt
    14281 ctcggcgccg gcgaccacgt agcgcagccg cccgaagtcg tacggatgcg cgaagcgcgc
    14341 gtagttgccg aggaaggtcg aggtgccgaa cagcaccgta cagttgcggt cgtaggtcag
    14401 ctccggcacc acgcggtagt gcagcggact cgggtacagg aacagcttgc agcccgagac
    14461 cagcggcagc agcgcgccgg cggtcaggcc gaagctgtga aagagcggca gcgccaccat
    14521 gaacttgtcg cgcggcgtga aatcggcgat cgcacagacc tgcgcgacgt tggcgagcag
    14581 cgaatcgtgc gaatgcacga cgcccttggg cttgccctcc gagcccgacg tgaacagcac
    14641 caccgccgca tcctgcggct gcacacgcgc atcgagcgcc aggcgcggcc acaacaggcg
    14701 cggcagcagc cgcagcttgt cggcgagccc catgccggcg cgcaggtctt cgagatacag
    14761 ccagcgcagc tcgccgagtt cgcccggcag gtgatcgagc ttgcccttgt cgaggaaggc
    14821 acgcgcggtg acgacggtgc gcacctccgc cgccgtgcag gccgcgcgca ggccgtcgac
    14881 gccggccgtg taattgagca tcgccggaat gcgcccgcgc aggctcagcc cgaggatcgc
    14941 cgccaccgtc gccgccgcgt tcggcagcag cagcccgacg cactcgccgc ggccggcgtg
    15001 gcgcgcgacc agcaggccga ggccgagcgc catcttcgtc atcgaggcgt aggtctcctc
    15061 gatcaggcgc agatcctcga cgatcggcgt gcgcgcgccg tgcgtgctgc gcgcatcgag
    15121 gaaggcctcg aacagcgtgc gcccggggcg cgtcagcacc agcatctcgg tcagcagcgc
    15181 acgcatgttc tggccggcga ccgcgcgccg gtcgcgcgcc ttcggccgat ccggcatcgg
    15241 cagcacgcgc ggtgcgagca cgtgcaggcg gatcttcggc agcagccgca gccggtagac
    15301 cccggccagc cgcccgaaga agctctgcgc cgccccggcg atgcgcaccg gcaccacggt
    15361 cgcgccggtc ttggcggcga cgaaggccgc gccgtcgtag accttcatca gcgagccggt
    15421 gacggtgatc cgcccctccg ggaagatcac caccggccgc ccggactcga ccaggcgcac
    15481 gatcgccttc agcgccagcg gattggccgg atcgacggcc agatgctcga tgtggcgcag
    15541 gatgcggccg aagaccggat gcacggcgac ctgcgtgtgc acgacgaaca ccggctcgac
    15601 cggcaggaac aggccgagca gcaggccgtc gaggaaggat tcgtggttgg cgacgatcag
    15661 cgtgcgtgca tgcccgaaca catcggtacg gccgcggatc tcgacgcgga acagcgtgcg
    15721 catcagcgcg cgcaacaggg atttcagcat ctgcgggact cccgctccgc ccgcggcccc
    15781 gcgggggagg gtgttcttgt cagggaaagc cggcgccgct gcgcgggcgc caggcgatgc
    15841 gcgtccggtc ttcggcccgc ggacgggcgg gaccgcgcgg gctcggccct tccccgcgcg
    15901 ggaaaagccc cggtcagaag gaaaagcggt cttcggcggc cgcgcgcgtt cagtcgatga
    15961 cgaccgccgt gccgctgacg gcgaccatca tcatgccgtt ctgctcgccg agcacttcat
    16021 agtcgatgtc gatgccgacc acggcattcg ccccgaggcg ctgggccgcc ctggtcagct
    16081 cggcaaacgc ttcctcgcgc gcatcgcgca gcacaccttc gtaggtgccg ctgcgcccgc
    16141 cgatgaagtc ggtgaggccg gcgaaccagt ccttgacgac attggccccg aggatggcct
    16201 cggcgctgac gatgccgcgg tactcgcgga tgcggtggcc ttcgagcgtc ggtgtggtgc
    16261 tcaggatcat cggcgtggcc tcgcgggcgg tggggacggg gagggtatag ccgaagaagc
    16321 ggcgtcgctg cgaacgcagg gcggtgtcga tgcgggaagc ccggcaggcg tgcgtccctg
    16381 cgcggcatcg gcacgtccac gcgcgtcatc tgcggaccca tgcgcggcat cggcacttcg
    16441 atgcgcgcca tgcgtgatcc gctgcccggc gtggcgcgac ggtggtcgcg catgaaaaac
    16501 cggggcggca agccccggtc gtggatgggc ggtatcaggg ccgatcggcg ggcggtatgc
    16561 acccggggca ggcgaagcgc ctgctcatcc gtgtcgaccg ttcgtctttc gggatgcacg
    16621 cgctgatgcc gggcaactgg cgtgtcgcgg aagaccggtg gcggctacgg gctgctcgcg
    16681 aagcatcgtc agcgttcacc gtgttcggca atccattgct tcagggcatc gttgatccgg
    16741 gtctgccagc ccgccccatc cgcccggaag tgcgcaagca catcggctga caggcgcagg
    16801 gtcgtgcgtt gcttcgtcgg cagtttctgt aatccgcgct ggcccggccg gcgcaggatc
    16861 ggtgcagtcg cgagttcttc gtccgtcatc ggcagcgcat ccggatcgtc ggcaccgatc
    16921 tcgtcgtccg ggatcgcgtt gacccgcgcc caatcgcttt ccgattctcc acgggcaatg
    16981 cgctgacggg cttcctcgct gctcagtcgt gtcagatttt cagttctggg cgttctgttc
    17041 ataaaaacgc acctcccgat ggttggcctt ccggaacgac acgatgcgga tgcagnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccac cgccacgtcc ggttccgtgc
    17221 cgctgcggtt caggcgctgg atcgcgaggt ccaggtcgag cagcagcgtg cgctgcgcgt
    17281 cgtcgcggat caggctctgc acccagaaca cgcaggcgat gcgcgcgccg cgcgtgaccg
    17341 gcgtcacgcg gtgcaggctg gtcgacgggt agatcaccag atcgccggcc ggcagcttga
    17401 cggtgtgcgt gccgtaggtg tcgtcgatgg tcagttcgcc gccgtcgtag tcctccggct
    17461 cgctgaggaa cagcgtcgcc gagacgtcgg tgcgcacgcg cgtgccgtcg ggcagcacgc
    17521 gcacggcgtt gtcgacgtgg ttgccgtagc tgccgccgcc ggcgtagcgg ttgatcatcg
    17581 gcgggaagat gcgtgccggc agcgtcgcgg tcaggaacag cgggttgcgc tccagcgccg
    17641 ccagcgcctg cgcggcgagt tcgcgggcga gcggatcgtc gtcggccagt tgctggttgt
    17701 gcttgacggc gagcgcctgg tgaccggcgc tgcgccggcc gtcgagccag tcggcgcggg
    17761 cgagacgttc gcgaaagccg gcgagccggt cgcggggcag gacttcgggg atgtgcagca
    17821 gcatcgggac gggtccgggc aggcggaaac ggggcgggct cgaagcccct ctcccgcgag
    17881 gggagagggg agggtcgaga cgcgctttat cgctttatta aggagggtca gaaccggtaa
    17941 tcgacgctca gcgtcgcggc acggccggtg ccggggatgg cgtggccggt gtagacggcc
    18001 tcgtaccact gcgtgtcggt caggttgacg acgttcaggc gcacgtcgaa atccggctgc
    18061 ttgtaggcga cttcggcatc ccagcgcgtg tagccgggca cgtagttggt gttggtgttg
    18121 ctgccgtagc gcttgccggc cccctgcacg ccgccgccga tctcccagcc gccgccgagg
    18181 tcgtaggtcg tccagacgct cagcgtatgg cgcggcgcat tggccgggcg cttgccgact
    18241 tcggccggat tgttcgactc ggtgatctcc gggtccatga acgccagacc ggcgaacacg
    18301 ctccactgcg gcgtgatgcg gccgatcacg tcgagctcga tgccgtcagt gacccagcgg
    18361 ccgtcgagca cgttgacgtt gagctgatcc ggatcgagcg tgcgggcgtt gatcttctcg
    18421 gtgcggaaca gcgccgtgcg caccgacagc gcaccgtcga ggaagtcgag cttggcgccg
    18481 atctcgaagt tgtggttctc ctccggatcg aggccggcgt tgttgctcga aatcgtcagc
    18541 gtctcggcgg tcgggttgaa cgaggtgccg aaggcggcgt agtaggactg cgtcgggctc
    18601 ggctgccaga tcacgccgat gcggccgctg gtcatcgtgt cggtgcgctt gcggtcatcg
    18661 tgcgtcagct gcgtgccggt cgccggggcg aaggtctcgg cgagcgaact ggcggcgaag
    18721 cggtcccagc gcaggccgcc gaccaccttc cattccggag tcagcgcgat ctcgtcgatc
    18781 gcgtagacgc cgaaggtgtt ggccgaggca tcggtgtagc tgctggtgcc gaccacgcgc
    18841 atcagcggcg ccggcgtgtc cggatcgggg tgggcgaggt tggtctgcgg tgcgccggtg
    18901 acctgatagc gcgtcgtggt cgtggtctcg cggccgatct cgacgccgcc gaccagcttg
    18961 tgctcgaagc tgccggtcgt gaacttcgcg gtgatgtcgg tctggttggc gagcgtcgtg
    19021 tcggtgccgt cgcggtacgg gcggctgcgg ttgacgctga tcagctcgta cggcgtgccg
    19081 tccggcgggt tgccggcgat gcgcggcgca ctcggggcga gcgagcggtc gtagcgcgcg
    19141 tagcgcgtcg agttgcggaa gctcaggctg tcgttgaagc ggtgatcgac cgtgatgctg
    19201 ccgatgtcga ccgtggtgtt ctcgaagtcg tggtcggcga ggccgtagaa gttcttcttg
    19261 tcgacatcga ccggcttgaa gttgcggtac gggacgccgt agtacgggac gttgtcctcc
    19321 ttctgccgga agtacgagaa gttgacctcg gtgtcggtgc cgagtccgag cgtcagcgac
    19381 ggtgcgatgc cgtagcggtc gaactgcacg tcctcgggcg tggcaccgga ctgctgccac
    19441 atgccgttga ggcgaaacgc cgcggtgctg gtgagcggct cgttgaaatc gaccgtcagc
    19501 cggcgcaggt cgagcgtgcc gaggctggcg ccgaccgtgt gcgcgctctc cagccacggg
    19561 cgcttgctgg tctggttgat cgcgccgccg gtggtgccgc ggccgaacac catcgacgag
    19621 gggcccttca gcacctcgac cgcctcgatg ttgaagatgt cgcgcgtgta cgagccgctg
    19681 tcgcgcacac cgtcgaggaa caggtcattg cgcgccgtga agccgcgcag gttgatgttg
    19741 tcgccggaat tgccaccctc gccgccgttg aagctgatgc ccgagacatt gcgcagcgcc
    19801 tcgcgcagcg tcgtcgcacc ctgctcctcg atgatctgct gcggcacgac ggtgatcgac
    19861 tgcgggatgt cgcgcagcgg ccccggcaga cgggtcaggc cggtgccgtc gcgcagatag
    19921 ccctgatcct gcggggcggt gacggtgact tcgggcaggg cggcgctgtc gccatcggcc
    19981 gcgcagacgc tgccgggcag cgcggcaccg agtgcgccga gcaggcagcc ggtctgcagc
    20041 ggcgtgcggg cggtcgaacg gatgccggcg ctcgggcggg cgagagggtg cggggcatgg
    20101 atcggcatga cggaaaactc ccggggcttt tgtgttgtgg cgccgtggcg ctgtggagtt
    20161 gcgggcagag cccacccgcc gcgcgcggcc ggcaagccgc gatcggtggg ggaaaaaaat
    20221 ccggagttcg ctgaaagccg gggctggtga tcggaactca ggccgatccc ggggcgcttc
    20281 ccggcgttcc gttcccgcgc cggcaggtgg cgcggggcgg ctgcggcgtc ctgccggctg
    20341 ccggcttcag tggatcaggc cgggcaggcc gagcagcagc gcgcgccaga ccgcgcattc
    20401 gctgccgtcg gtcggccgcg gcggggcgcc gagggcggcg agcaggcggc cgtcggcggc
    20461 atagagctcc agcgtgctgc gcgcgcggct gcgcaggcct tcgggtcggt gcacgaccca
    20521 ggtctgcgcg atcgcgcgtt cgtcgagctc ggccagaaat ccggcaccgg acagccgcag
    20581 ccggccgccg agccggtcgg cgcgcagcag cgccgcg
//
LOCUS       DAOQIE010000022        20250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1190_length_20250_cov_7.771181, whole genome shotgun sequence.
ACCESSION   DAOQIE010000022 DAOQIE010000000
VERSION     DAOQIE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 20250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20250
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1190_length_20250_cov_7.771181"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1566)
                     /locus_tag="PK144_02675"
     CDS             complement(253..1566)
                     /locus_tag="PK144_02675"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNF64661.1"
                     /translation="MNPVKVGLLGLGTVGGGTVNVLKRNAEEISRRAGRGIVITHAAA
                     KNLDSTRADTTGIRLGSDAYEVVNDPEVAIVVELIGGYEPARTLVLQAIRNGKHVVTA
                     NKALIARHGNEIFAAAREHGVTVAFEAAVAGGIPVIKAIREGLAANRIEWLAGIINGT
                     GNFILTEMRDKGRDFADVLAEAQALGYAEADPTFDVEGIDAAHKLTILASIAFGVPLQ
                     FEKVYTEGITRITREDVAYAEQLGYRIKHLGLARATDEGIELRVHPTLIPERRLIANV
                     DGVMNAVLIKGDAVGPTLYYGAGAGAEPTASAVIADIVDVVRALTSDPGNRVPHLAFQ
                     PDALSDKPVLPVEDIETAYYLRLQAADRPGVLAEVTRSFADAGISIEAILQKEPPAGS
                     PDATVIILTQRVRERQMNEAIARIEALDAITGTVTRIRLEHLASN"
     gene            complement(1615..2811)
                     /gene="alaC"
                     /locus_tag="PK144_02680"
     CDS             complement(1615..2811)
                     /gene="alaC"
                     /locus_tag="PK144_02680"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HNF64662.1"
                     /translation="MSAPSQEFPRISRLPPYVFNIVNELKAKARARGEDIIDFGMGNP
                     DRPTPQHIVDKLVEAAQRDDTHRYSVSKGIPRLRRAICNWYKRRFDVELDAESEAIVT
                     IGSKEGLSHLALATMGPGDTVLVPNPAYPIHPYGFVIAGADIRHVPMVPGVDFFEELQ
                     KAIRESWPKPKMLVLNFPGNPTAHCVELGFFERVIAIAREHGIWVVHDLAYADIVFDG
                     YVAPSILQVPGAKEVAVEFFTLSKSYNMPGWRVGFMCGNPVLVHALARMKSYMDYGTF
                     TPIQVAAISALEGPQDCVAEICSTYRSRRDVLCDGLNAIGWPVEKPKATMFVWAKIPE
                     AYRAAGSLEFAKKLIAEARVAVAPGIGFGDYGDDHVRFGLIENEHRTRQAIRGIRDMF
                     RKDSRA"
     gene            3040..3561
                     /locus_tag="PK144_02685"
     CDS             3040..3561
                     /locus_tag="PK144_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF64663.1"
                     /translation="MLSEVTRDFILHWGEMGTRWGVNRSVAQIHALLFLAEAPLPADA
                     IVESLGLARSNVSNSLKELQSWQLVQVRHVMGDRREHFVAVTDIAEVFRLIVEGRKQR
                     EIDPTLQALRRFASAAEAGHEPHVQTRIAEVLAFVGMLDQWYATMTGARGTGPIDYLR
                     MAQEIHRMLHRRP"
     gene            3676..4968
                     /locus_tag="PK144_02690"
     CDS             3676..4968
                     /locus_tag="PK144_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF64664.1"
                     /translation="MHVLLIGAGGFLGRHLAAALIAAGHTVTGTGRRGAPPPGHAVQH
                     WRRLELTAADNAADWPALLDGVDAVVYAAGLLRERRRGEFDAIHGRAPIALAGAAAAS
                     AGIRRIVLISALGAGEAVDAGFLGSRVAAEKALLALTPGVGVVLRPSLVYGVDGRSTG
                     WLRRLALLPLTPLPGDGRQPLQPLHVADLCAAVCRLLQPDAPVPPGGIIEAVGPAPLT
                     LRAYLAALRHGLGLPAAPVLPVPVGLLRLAARWLAPWRAQPLCPETLTLLLRGNTGDP
                     AALTALLDRPPRPVPAFAAGEHQSAERADSAAALAAGLARAALALIWAAGGLASLLPG
                     GRSAGFALLAQLGVHGPLAWLLLLGGAGLDLAFALLTLHPAAGRRLWAAQAAIVALYG
                     AIAAVGLPGLLADPLGAIVKNLALLALLGQLALHAEPD"
     gene            4968..5441
                     /locus_tag="PK144_02695"
     CDS             4968..5441
                     /locus_tag="PK144_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNF64665.1"
                     /translation="MDYGTLKTLHVLSSTLLFGTGLGSAFYLWRAARHGDAAQFAFVA
                     RQVVLADWLFTATAVVFQPLSGWLLARTAGHPLDAGWLQASYGLYLLAGACWLPVVWL
                     QYRLRDLARDAARLQQPLPARWHHCLCLWFVLGWPAFGAVVLIFWLMVNRPGFGA"
     gene            5454..5879
                     /locus_tag="PK144_02700"
     CDS             5454..5879
                     /locus_tag="PK144_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNF64666.1"
                     /translation="MHGNDPQADSPAVPSTLTLFYDGDCPICRAEVRWLKRHDRHRRL
                     HYVDVAPVDFHAPAPLPARARLLERIHLLDAGGQVHVGMAVFRLAYRAVGLSWLVAPT
                     GWPLLRPLFDRLYGCFARHRIGLGRRFGRPCDSESCRLR"
     gene            6016..6390
                     /locus_tag="PK144_02705"
     CDS             6016..6390
                     /locus_tag="PK144_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNF64667.1"
                     /translation="MRLALDQDSNVHLIRNYGPGYVTVDAEQRLTRSFALAPDALLPD
                     WGPEGFEALAAEHFEALLALKPELVLLGTGARQRFPHPRLSRALTEAGIAVDAMDTAA
                     ACRTFNIVVAEGRRAVAALLMI"
     gene            complement(6411..7472)
                     /gene="rpoS"
                     /locus_tag="PK144_02710"
     CDS             complement(6411..7472)
                     /gene="rpoS"
                     /locus_tag="PK144_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNF64668.1"
                     /translation="MPRRTPDLGGFTEEPEAFAVVEEEEETLLDTDEAGPDADDVAAE
                     ALVEAGLDEAEPVEDDERTLSKTPLYGKDFSQDELDATRLYLGEIGFSPLLTAQEEVY
                     FARRALRGDEAARKRMIESNLRLVVKISRRYMNRGLTLLDLIEEGNLGLIHAVEKFDP
                     ERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIIKEINVYLRTARQLAQALDHEPS
                     AEEIAQSMDKPVDEVKRMLGLNERIASVDTPIGKDEDRFLLDAIPDDNNPDPFQVLQD
                     EDLNTKLEYWLGQLNDKQRIVVERRFGLGGKERATLEEVGNEIGVTRERVRQIQIDAL
                     RRLRKILEAEGFSEDVLYG"
     gene            complement(7622..8413)
                     /locus_tag="PK144_02715"
     CDS             complement(7622..8413)
                     /locus_tag="PK144_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNF64669.1"
                     /translation="MPFLLHRYRAPVTRALAALLLAGCAAYGPERPSEYVVVRGDTLY
                     AIAWRFGIERNDLVRWNGITNPDRILVGQRLVLAPPETGAPAMRNAGPVVVAAEARDS
                     PRSTPVRSARVPRRTTAGVAAARPVAARASADDGDDSLARGPWRWPVRGELLRRYDAD
                     GPGRKGIEIGAGLGQTVSAAQGGEVVYSGSGLPGYGRLIIIKHSESMLSAYGFLGKIL
                     LNEGDKVRAGQAIGEVGTSSENRPALHFEIRQNGRPVDPLRYLPG"
     gene            complement(8438..9082)
                     /locus_tag="PK144_02720"
     CDS             complement(8438..9082)
                     /locus_tag="PK144_02720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNF64670.1"
                     /translation="MTSARTRTRLVARLREQGIDDERVLAVMRETPRHLFIEEALASR
                     AYEDTALPIGLGQTISQPYIVARMTQALLAGREALPLRVLEIGTGSGYQSAVLAPLVG
                     QVYSVERIERLSLRARERLAALGLRNVHLKHADGSIGWPGCGPYDGILVTAAAERLPP
                     ALPEQLATGGRLVAPLGPAGGVQELLIVERKAIGYETHSLGAVVFVPLLAGVQP"
     gene            complement(9150..9899)
                     /gene="surE"
                     /locus_tag="PK144_02725"
     CDS             complement(9150..9899)
                     /gene="surE"
                     /locus_tag="PK144_02725"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNF64671.1"
                     /translation="MRILVSNDDGYLAPGIRALAQALAGIAEITVAAPDRNRSGASNS
                     LTLDRPLSVVRHPDGVWSLDGTPTDCVHVALTGLMEHEPDMVVSGINAGANLGDDVLY
                     SGTVAAAMEGRFLGRPAMAVSLCAYEPDHLDTAARVALHLVGRLCRQPLPADTILNVN
                     VPAVPWSELRGWQVTRLGHRHKAEPAVCTRDPRGRPIWWIGPAGAESDAGPGTDFQAI
                     REHCVSVTPIQVDLTRHAALDTVARWLDAPA"
     gene            10019..10564
                     /locus_tag="PK144_02730"
     CDS             10019..10564
                     /locus_tag="PK144_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNF64672.1"
                     /translation="MPADRPEPPADEVEAFRQAIGPIEPHRCDLVEPWRAPRPPLPLQ
                     KLADEKQVLRDMVSDHFEPADLETGEHLSYSREGVQDGVLRKLKRGQYSLGAQLDLHG
                     LTWAEARVTVHAFLSLVQRDRVHCVRIVHGKGNRSRHLGPVLKQKLNVYLRLRDDVLA
                     FCSARPDDGGTGALYVLLRSR"
     gene            complement(10574..11677)
                     /locus_tag="PK144_02735"
     CDS             complement(10574..11677)
                     /locus_tag="PK144_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF64673.1"
                     /translation="MSASGRRIGFVLCSDAAAPLPSTRISVFNVLPALRAAGYDPRIA
                     FAPPQPTERPDVSGLAARLIADGIDIAYFQKVHGPGVRAEIETLRQAGVRTVYGVCDR
                     IDDDMVRLTDATVIVTDYLKQQHAPELQARIHVVHDGIEHPEVRCREDIAAGDGRLRA
                     ILVTSGSPETVPVLGRPPRWLALTVVGQYPERETPLQALRARLRRVRHAPPGERLERL
                     RGYAFTARAWQPQRVYEDLAGAEIGIIPVDMRPDPLPGRQVSYWQVKSENRLTLKMAA
                     GLAVVASPVPSYLDIIEQGVNGYIAHSRADWLAALDALRDPQHRHSVGAAARASVLQR
                     YSIDEQARRLIAVFDGLRGTSQPAGDAALLRMP"
     gene            complement(11682..12407)
                     /locus_tag="PK144_02740"
     CDS             complement(11682..12407)
                     /locus_tag="PK144_02740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF64674.1"
                     /translation="MMTAAAGTLLNRESAYTTPRADVSALVPREAMRILDVGCSNGAL
                     GASLRAEVAGRRVVGIELDAGFARQAALLLDRVEQADLDAFDWSDGLGDERFDCIVFA
                     DVLEHLVDPQRHLRGALERLDAGGCIVLSLPNIRHVSALASIYGHGRFPRRDRGIFDA
                     THLRWFTIADGRSLLADTGLQIDAEGFVLRAGDRGGGFLNRMLNRLPDALQRWAPVRE
                     FLTYQFSLRARRPAALPETVPMS"
     gene            complement(12459..13520)
                     /gene="truD"
                     /locus_tag="PK144_02745"
     CDS             complement(12459..13520)
                     /gene="truD"
                     /locus_tag="PK144_02745"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNF64675.1"
                     /translation="MNTTVDELPHAHGRPPLCGRIRCAPEDFIVIEELGWQPDGSGEH
                     VFLYVRKSGQNTDWVARRLAEVCEVRADAVSYAGLKDRHAVTEQWFSVQMPGREAPMP
                     CGFTGSRGIDGVEVLAVTRNSRKLRRGALAGNRFRIVVRELDGPPAAVLDRLPAIAAR
                     GVPNWFGEQRFGRDGGNLERARRLLAGTLRERDRHKRGLYLSAARSHLFNRYLAARIS
                     DGSWDQALPGEVLMLDGSHSVFACPQPDATILERLARHDLHPSGPLWGAGESLATAAA
                     LAGETALAAAEAQLCAGLAAEGLKQERRALRLVPAQLHGEAIGGDAVRLDFRLPAGAY
                     ATTVLHELVGNPEAQDDML"
     gene            complement(13517..13999)
                     /gene="ispF"
                     /locus_tag="PK144_02750"
     CDS             complement(13517..13999)
                     /gene="ispF"
                     /locus_tag="PK144_02750"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018862913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNF64676.1"
                     /translation="MRIGHGFDVHAFGPGDHVTLGGVRIPHTHGLIAHSDGDVLLHAL
                     CDALLGAAALGDIGRHFPDSDERYRGIDSRRLLHRVVELIRTEGYAVGNVDATLIAQA
                     PRMAAHIEAMREHIAADLGVNRDAVNVKATTTERLGFTGRGEGIAVEAVVLLVEAAVL
                     "
     gene            complement(14095..14463)
                     /locus_tag="PK144_02755"
     CDS             complement(14095..14463)
                     /locus_tag="PK144_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64677.1"
                     /translation="MKITLNLRPLMLAGGLSVLALAGCTTVVRPGPAVVERPAPRPVV
                     VEERVEYVRERPPPPRREARPYRPSPNADWVPGRWVWRNGWDWQPGHWENRPQPRARW
                     QAGHWESRPSGWIWVEGGWR"
     gene            complement(14537..15232)
                     /gene="ispD"
                     /locus_tag="PK144_02760"
     CDS             complement(14537..15232)
                     /gene="ispD"
                     /locus_tag="PK144_02760"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNF64678.1"
                     /translation="MARCFAIVPAAGVGRRMGAAIPKQYLPLAGRCVLEHTLERLLAE
                     PRIERIVVALGPEDGWWPALKLSDPARIRRVDGGTERADSVLNGLRALEGLAGTDDWA
                     LVHDAARPCLAAEDLARLFTALADDPLGGLLAVPVRDTMKRAAADGCVEHTVERERLW
                     HALTPQMFRYGRLRDALEAAAAAGAVITDEASAIERAGLRPRLVEGRADNLKITRPED
                     LALAAFFLAAGRP"
     gene            complement(15232..15693)
                     /gene="ftsB"
                     /locus_tag="PK144_02765"
     CDS             complement(15232..15693)
                     /gene="ftsB"
                     /locus_tag="PK144_02765"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNF64679.1"
                     /translation="MRLFLAALIALLLGLNYLLWMTDDRGLRQMRTLERAVADQKSEN
                     TELADRNKSLEAEVRDLKQGNAAIEERARTELGMVRGDETFYRILDEAPSSPPSALTK
                     PGTAIHARPQAPSAPAAAAPAAPKPDAKPAAKPAVPRPPASKPAPDSGRPD"
     gene            complement(15776..17068)
                     /gene="eno"
                     /locus_tag="PK144_02770"
     CDS             complement(15776..17068)
                     /gene="eno"
                     /locus_tag="PK144_02770"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF64680.1"
                     /translation="MSAIQSLIGREILDSRGNPTVQVEVELASGARGTAAVPSGASTG
                     TREAIELRDGDAGRYLGKGVLKAVGHVNGEILKAVRGMEAADQAALDARLIELDGTAN
                     KGRLGANALLGVSLAVAHAAASEKRVPLYRHINPDGPYVLPTPMMNILNGGAHADNSV
                     DIQEFMVMPVGAPSFSEALRCGAEIFHALKKVLNGRGLATGVGDEGGFAPNLPSNEAA
                     LEVVMEAIGKAGYRAGDDVMLALDCASSEFYRNGRYTLEADGKAYSSLEWAGVLADLA
                     GRYPIISIEDGMDESDWAGWAELTRLLGERIQLVGDDLFVTNTEILQRGIREGIANSI
                     LIKVNQIGTLTETLNAIRMADEAGYSSISSHRSGETEDTTIADLAVATTARQIKTGSL
                     SRSDRVAKYNRLLVIESQLGADAVYAGRKAFPRKLKLG"
     gene            complement(17175..18011)
                     /gene="kdsA"
                     /locus_tag="PK144_02775"
     CDS             complement(17175..18011)
                     /gene="kdsA"
                     /locus_tag="PK144_02775"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNF64681.1"
                     /translation="MKLCGFEVGLDRPLFLIAGPCVIESRELAFETAGRLKEMCAALG
                     VPFIYKSSFDKANRSSHASYRGPGLEAGLAILEAVRRELDVPVLTDVHEDTPLAEVAA
                     VVDVLQTPAFLCRQTNFIQAVARAGRPVNIKKGQFLAPWDMKNVADKALATGNTQIMV
                     CERGVSFGYNNLVSDPRALAVMRDTGCPVVFDATHSVQLPGGQGTSSGGQRQHVPVLA
                     RAAVAVGVAGLFMETHPQPEQALSDGPNAWPLGRMQGLLETLVELDALVKRRGFDEND
                     PG"
     gene            complement(18008..19645)
                     /locus_tag="PK144_02780"
     CDS             complement(18008..19645)
                     /locus_tag="PK144_02780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNF64682.1"
                     /translation="MTRFIFVTGGVVSSLGKGIAAASLAAILEARGLQVTMIKLDPYI
                     NVDPGTMSPFQHGEVYVTEDGAETDLDLGHYERFVRMTATRRNNFTTGRIYENVIRKE
                     RRGDYLGGTVQVIPHITDEIKRCVRLGAGNADIVMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRERALFMHLTLLPYIATAGELKTKPTQHSVKELRSIGIQPDILLCRCEHEIPAE
                     ERRKIALFTNVEAKAVFSAVDADTIYRIPLLLHQQGLDDVVCQKLGLRADPANLDEWT
                     RVVGQIEAARGDTIEIAMVGKYVDLADAYKSLNEALFHAGFHTGHKVAIRYIDSEQLE
                     REGTGCLAGMHAILVPGGFGERGVEGKIAAVRHAREQRIPYLGICLGMQVAVIEYARN
                     VAGLEGAHSTEFRKNATHPVIALITEWTNREGAVERRSEESDLGGTMRLGAQPARLVE
                     GTLAHRVYGKDVIVERHRHRYEFNDGYVPVLEKSGLRFSGFSQDGNLVEMIELPDHPW
                     FLACQFHPEFTSTPRYGHPLFRAFVEAAVAHREGKLA"
     gene            complement(19642..>20250)
                     /gene="tilS"
                     /locus_tag="PK144_02785"
     CDS             complement(19642..>20250)
                     /gene="tilS"
                     /locus_tag="PK144_02785"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNF64683.1"
                     /translation="HIPQLDPELPIPPAARLDTVLRDLLPARRDAEPCVCWPGAEMHR
                     FRERLHVFAPLPALPDALCLDWPPEGGEISIAGLGTLVWQPASGAGLDAARLAGRRVT
                     LRLRQGGERFRPAGRRHAQELKKLLQAAGIAPWLRARLPLLYIDEELVAVAGLGIAEG
                     WLAGPDAPGRVPAWQTQAPSSTMPRFVAAPLGAREAGLEHFP"
BASE COUNT         2958 a         7057 c         7107 g         3128 t
ORIGIN      
        1 cctgtcgaac acgctgaagg ccgacaaccg ggcaggcagc ggcggcagcg gcgcgcgcga
       61 gcagccggcg cagcatcggc gcgcgcggct gcggcggcgc gtagccggcg agcgtatccc
      121 agcccggcag caggccgggc acgatcagca gcagctccat cgcgggctcc gtggaatccg
      181 gcagcggcgc gccggccaca aacgcgcagg cccggcacgc ggccgggcct gcgcagcgga
      241 cggcgcggag cctcagttcg aggcgaggtg ttcgaggcgg atgcgcgtga ccgtgccggt
      301 gatcgcgtcg agtgcctcga tgcgcgcgat cgcctcgttc atctgccgct cgcgcacgcg
      361 ctgggtcagg atgatcacgg tcgcgtcggg gctgccggcc ggcggctcct tctgcaggat
      421 cgcctcgatg ctgatgccgg catcggcgaa gctgcgcgtg acctcggcga gcacgcccgg
      481 gcggtcggcg gcctgcaggc gcaggtagta cgcggtctcg atgtcctcga ccggcagcac
      541 cggcttgtcg gacagcgcat cgggctggaa agccaggtgc ggcacgcggt tgccggggtc
      601 ggaggtcagc gcgcgcacga catcgacgat gtcggcgatc accgccgagg cggtcggctc
      661 ggcgccggcg ccggcgccgt agtacagcgt cggaccgacc gcatcgccct tgatcagcac
      721 cgcattcatg acgccgtcga cgttggcgat cagccgccgc tccgggatca gcgtcggatg
      781 cacgcgcagc tcgatgccct cgtcggtcgc acgcgccagc cccagatgct tgatgcggta
      841 gccgagttgc tcggcgtagg cgacatcctc gcgcgtgatg cgcgtgatgc cctcggtgta
      901 gaccttctcg aactgcagcg gcacgccgaa ggcgatcgac gcgaggatgg tcagcttgtg
      961 cgcggcgtcg atgccctcga catcgaaggt cggatcggcc tcggcgtagc cgagcgcctg
     1021 ggcctcggcg agcacgtcgg cgaaatcgcg ccccttgtcg cgcatctcgg tcaggatgaa
     1081 gttgccggtg ccgttgatga tgccggccag ccattcgatg cggttggcgg cgagcccctc
     1141 gcggatcgcc ttgatcaccg ggatgccgcc ggcgacggcc gcctcgaagg ccaccgtcac
     1201 gccgtgctcg cgcgccgcgg cgaagatctc gttgccgtgg cgggcgatca gcgccttgtt
     1261 ggcggtgacg acgtgcttgc cgttgcggat cgcctgcagc accagcgtgc gcgccggctc
     1321 gtagccgccg atcagctcga cgacgatggc gacctcggga tcgttgacga cctcgtaggc
     1381 atcgctgccc aggcggatgc cggtcgtgtc ggcgcgggtc gaatcgaggt tcttcgcggc
     1441 ggcatgcgtg atgacgatgc cgcggccggc gcgccggctg atttcctccg cattgcgctt
     1501 gagcacgttg accgtgccgc ccccgaccgt acccagaccc agcagaccga ctttgacggg
     1561 attcaccgtg taaacctcat aagaaacgga cttgaacgga tgcacgcgcc gctgtcaggc
     1621 gcggctgtcc ttgcggaaca tgtcgcggat gccgcggatg gcctgccgcg tgcgatgttc
     1681 gttctcgatc aggccgaagc gcacgtgatc atcaccgtag tcgccgaagc cgatgcccgg
     1741 agccacagcg acgcgggcct cggcgatcag cttcttcgca aactccagcg aacccgccgc
     1801 gcggtaggcc tcgggaatct tcgcccagac gaacatcgtc gccttcggct tctcgaccgg
     1861 ccagccgatg gcgttgagac cgtcgcagag cacgtcgcgg cgcgaacggt aggtgctgca
     1921 gatttcagcc acgcagtcct gcggcccttc gagcgcgctg atcgcggcga cctggatcgg
     1981 cgtgaacgtg ccgtagtcca tgtaggactt catgcgcgcc agcgcatgca cgagcaccgg
     2041 attgccgcac atgaagccga cgcgccagcc cggcatgttg taggacttcg acagcgtgaa
     2101 gaactcgacc gcgacctcct tcgcacccgg cacctgcagg atcgacggcg ccacgtagcc
     2161 gtcgaagacg atgtcggcat aggcaaggtc atgcacgacc cagatgccgt gctcacgggc
     2221 aatggcgatg acgcgctcga agaagccgag ttcgacgcag tgcgcggtcg gattgccggg
     2281 gaagttcagc accagcatct tcggcttcgg ccaggactcg cggatcgcct tctgcagctc
     2341 ctcgaagaag tccacgcccg gcaccatcgg cacgtggcgg atgtcggcac cggcgatcac
     2401 gaaaccgtac gggtggatcg gataggccgg attcggcacc agcaccgtat cgcccggccc
     2461 catcgtcgcc agcgccagat gcgacaggcc ctccttggag ccgatcgtga cgatggcctc
     2521 gctctcggca tcgagttcga catcgaaacg gcgcttgtac cagttgcaga tcgcccggcg
     2581 caggcgcggg atgcccttcg agaccgaata gcggtgcgtg tcgtcgcgct gcgcggcctc
     2641 gaccagcttg tcgacgatgt gctgcggtgt cggccggtcc ggattgccca tgccgaaatc
     2701 gatgatgtcc tcgccgcgcg cgcgcgcctt cgccttcaac tcgttgacga tgttgaagac
     2761 atacggcggc aggcggctga tgcgcggaaa ttcctgactg ggcgcagaca tcgtgcaggc
     2821 ctcgaggact agccctgcgg cgggcgaagg accgggaacg ttacggaccg ccctgcaccc
     2881 tgtcaaacca gcgcaggcag cggctgcgca gtacgaaacc cctgcccctg ccatcatcgg
     2941 caaccggcct gcggccgccg ggtgcgccga tccgcagggc ttgccgggaa gcaatcgtta
     3001 cggtaatttc tgtcattaat gaaataacgg aatcccgcca tgctctccga ggtcacgcgc
     3061 gacttcatcc tgcactgggg cgagatgggc acgcgctggg gcgtcaaccg cagcgtcgcg
     3121 cagatccatg cgctgctgtt ccttgccgag gcgccgctgc cggccgatgc catcgtcgag
     3181 agcctcgggc tggcgcgctc gaacgtcagc aacagcctga aggaactgca gtcttggcag
     3241 ctcgtgcagg tgcgccacgt catgggtgac cggcgcgagc atttcgtcgc cgtcaccgat
     3301 atcgccgagg tgttccggct gatcgtcgaa ggtcgcaagc agcgcgagat cgatccgacg
     3361 ctgcaggcgc tgcgccgctt cgcgagcgcg gccgaagccg ggcatgaacc ccacgtgcag
     3421 acccgcatcg ccgaagtgct ggccttcgtc ggcatgctgg atcagtggta tgcaacgatg
     3481 accggcgccc ggggcaccgg gccgatcgat tacctgcgca tggcccagga gattcaccgc
     3541 atgctgcacc ggcgcccctg atgctgtccg ccgccagtcc tttggcggat ttttttggca
     3601 tgccgatttc tgttaattca gaaattacgg acatcacatg aacaacttgc ccgaatcagg
     3661 ctttgtggag gcacgatgca tgtcctgctg atcggcgccg gcggcttcct cggccggcat
     3721 ctggctgcgg cgctgatcgc cgccggccac accgtcaccg gcaccggccg ccgtggcgcg
     3781 ccaccgcccg gccatgccgt gcagcactgg cgcaggctcg aactgacggc cgcggacaat
     3841 gctgccgact ggccggcgct gctcgacggc gtcgatgccg tcgtctacgc cgccgggctg
     3901 ctgcgcgagc gcaggcgagg cgagttcgac gcgatccacg gtcgcgctcc gatcgcgctg
     3961 gccggcgctg cggcagcgag cgccggcatc cgccgcatcg tgctgatctc ggcgctcggc
     4021 gccggcgagg ccgtcgatgc cggctttctg ggcagccggg tcgctgccga aaaggccctg
     4081 ctcgcgctca ctccgggggt tggcgtcgtg ctgcggccct cgctggtcta cggcgtcgat
     4141 ggccgcagca ccggctggct gcgccggctc gccctgctgc cgctgacgcc gctgccgggc
     4201 gacggccgcc agcccctgca gccgctgcac gtcgccgatc tgtgcgcggc ggtctgccgg
     4261 ctgctgcagc ccgatgcgcc cgtcccgccc ggcggcatca tcgaagcggt cggccccgcg
     4321 ccgctgacgc tgcgcgccta cctcgccgca ctgcgccacg gcctcggcct gccagcggcg
     4381 ccggtgctgc cggtgccggt cgggctgctg cgcctcgcag cccgctggct ggcaccctgg
     4441 cgcgcgcagc cgctctgccc ggaaacgctg acgctgctgc tgcgcggcaa caccggggat
     4501 ccggcggcgc tgacggcgct gctcgaccgg ccgccgcgac cggtgccggc gttcgcagcc
     4561 ggcgaacacc agagtgccga gcgtgcggac agcgccgcag cgctcgcagc cggcctggcg
     4621 cgggcagcgc tggcgctgat ctgggccgcc ggcggactgg cttcgctgct gccaggcggc
     4681 cggtccgccg ggttcgcgct gctggctcaa ctcggtgtcc acggcccgct cgcctggctg
     4741 ctgctgctcg gtggcgccgg tctcgatctg gcgttcgcgc tgctgacgct gcacccggca
     4801 gccggtcgcc ggctgtgggc tgcgcaggcg gcgatcgtcg cgctgtacgg cgcgatcgct
     4861 gccgtcggcc tgcccggcct gctcgccgat ccgctcggcg ccatcgtcaa gaacctcgcg
     4921 ctgctggcac tgctcggcca gctcgcgctg cacgcggagc cggactgatg gactacggca
     4981 cgctgaagac cctgcacgtg ctgtcctcga cgctgctgtt cggcaccgga ctcggcagcg
     5041 cgttttatct gtggcgcgcc gcacgccacg gcgatgccgc gcagttcgcc ttcgtcgccc
     5101 ggcaggtcgt gctcgccgac tggctgttca cggcgaccgc cgtcgtgttc cagccgctga
     5161 gcggctggct gctcgcgcgc acggccggcc atccgctgga cgccggctgg ctgcaggcga
     5221 gctacggcct gtacctgctc gccggtgcct gctggctgcc ggtggtctgg ctgcagtacc
     5281 ggctgcgtga tctggcccgt gacgccgcgc gtctgcagca gccgctgccg gcacgctggc
     5341 atcactgcct gtgcctctgg ttcgtcctcg gctggccggc cttcggggcg gtcgtgctga
     5401 tcttctggct gatggtcaac cgccccggct tcggggcctg aaggagccca cacatgcacg
     5461 gcaacgatcc gcaagccgac agcccggctg tcccgtccac cctgacgctg ttctacgacg
     5521 gcgactgccc gatctgccgc gccgaagtgc gctggctcaa gcgccatgac cggcaccggc
     5581 ggttgcatta tgtcgacgtg gcgccggtgg attttcacgc tcccgcgccg ctgccggcgc
     5641 gcgcgcggct gctcgaacgc atccatctgc tcgatgccgg cggccaggtg catgtcggca
     5701 tggcggtgtt ccgccttgcg taccgtgcgg tcggcctcag ctggctggtg gcgccgaccg
     5761 gctggccgct gctgcgcccg ctgttcgacc gtctgtacgg ctgcttcgcg cgccaccgca
     5821 tcggcctcgg ccggcggttc ggccgcccct gcgacagcga aagctgccgg ctgcgctgac
     5881 cggcacccgg cagccggtgg aacgccagga cagcggcggg catcgaaaac cgggtcgacc
     5941 ggcttgagtc gccggcccgg tcgccggcat cgtaggcccc ggcgatcccc gcccagccat
     6001 ccgaggaggc cgcccatgcg tcttgcgctc gaccaggaca gcaacgtcca cctgatccgc
     6061 aactacggtc ccggctacgt caccgtcgat gccgagcagc gcctgacgcg cagcttcgcg
     6121 ctggcgcccg acgcgctgct gcccgactgg ggtccggagg gtttcgaggc tctggcagcg
     6181 gagcacttcg aggcgctgct ggcgctgaag cccgaactgg tgctgctcgg caccggcgcg
     6241 cgccagcgct tcccgcatcc gcgcctgtcg cgggcgctga ccgaagccgg cattgccgtc
     6301 gatgcgatgg ataccgcggc agcctgccgc accttcaaca tcgtggtcgc ggaaggccgc
     6361 cgggccgtgg cggcactgct gatgatctga aacggagccc gcgacggccc tcagccgtac
     6421 agcacgtcct cggagaagcc ttcggcttcg agaatcttgc gcagccgccg cagcgcgtcg
     6481 atctggatct ggcgcacgcg ctcgcgcgtg acgccgatct cgttgccgac ttcctccagc
     6541 gtcgcccgct ccttgccgcc cagcccgaaa cggcgctcga cgacgatgcg ctgcttgtcg
     6601 ttcagctggc cgagccagta ttcgagcttg gtattgagat cctcatcctg caggacctgg
     6661 aagggatccg gattgttgtc atccggaatc gcatcgagca ggaaacgatc ctcatccttg
     6721 ccgatcggcg tatcgaccga ggcgatgcgt tcattcaggc cgagcatgcg tttgacttcg
     6781 tcgacgggct tgtccatcga ctgcgcgatt tcctcggccg agggctcgtg atcgagtgcc
     6841 tgcgcgagtt gtcgggccgt acgcagataa acgttgattt ccttgatgat gtgaatcggc
     6901 agccggatcg tgcgcgtctg gttcatgatc gcgcgctcga tggtctggcg aatccaccag
     6961 gtcgcatagg tcgaaaaccg gaatccgcgc tcgggatcga atttctcgac ggcgtgaatc
     7021 aggccgagat tgccttcctc gatcaggtcg agcagcgtca ggccgcgatt catgtaacgg
     7081 cgagaaatct tcacgaccag acgcagattg ctttcgatca tgcgcttgcg tgcggcttca
     7141 tcgccacgca atgcccggcg cgcgaaatag acttcctcct gcgcggtcag cagcggcgaa
     7201 aagccgatct cgccgagata cagccgcgtg gcatcgagtt cgtcctggct gaaatccttg
     7261 ccgtacagcg gcgtcttgct gagcgtgcgc tcgtcatcct cgaccggctc ggcctcatcc
     7321 agaccggcct cgaccagtgc ttcggccgca acgtcatcag catcgggacc ggcttcatcg
     7381 gtatcgagca aggtttcctc ttcctcctcg acgaccgcaa aagcttcggg ttcttcggta
     7441 aatccgccca gatcgggagt acgacgcggc atggaaacct cctgacaccc tgctcggctc
     7501 tggtggtggg gaatatgtcc ttttcttaat ctttatctgc gcaggcttca gccggccttg
     7561 cgtcatcgag gcgcaatccg ccgccccccg acccgattgt cggggtggac ggtcaggaag
     7621 ttcaacccgg caaatagcgg aggggatcga ccggccggcc gttctggcgg atttcgaaat
     7681 gcagcgccgg acgattctcg ctgctggtgc cgacctcgcc gatcgcctga ccggcgcgga
     7741 ccttatcacc ctcattcaga aggatttttc cgagaaatcc ataggcgctg agcatggatt
     7801 cggaatgctt gatgatgata agtcggccat atccgggaag tccactgccg ctatagacga
     7861 cctccccgcc ctgcgcggcg ctcacggtct ggccgagacc ggcaccgatc tcgatgccct
     7921 tgcggccggg gccgtcggca tcgtaacggc ggagcagctc gccgcgcacc ggccagcgcc
     7981 aggggccgcg cgcgagcgaa tcatcgccgt catcggccga tgcgcgtgca gccaccggcc
     8041 gtgccgcggc gacgccggct gtcgtgcgcc gcggcacgcg cgcgctgcgc accggtgtgc
     8101 tgcgcggact gtcccgggct tcggcagcga cgaccaccgg accggcgttg cgcatcgccg
     8161 gcgcgccggt ctcgggcggg gcgagcacga gccgctggcc gaccaggatg cggtcgggat
     8221 tggtgatgcc gttccagcgc accagatcat tgcgctcgat gccgaagcgc caggcaatcg
     8281 catacagcgt gtcgccgcgc acgaccacgt attcggacgg tcgctcggga ccgtaggccg
     8341 cacagccggc gagcagcagg gcggcgagcg cccgcgtcac gggcgcacga tagcggtgaa
     8401 gcaagaacgg catgggcatg tccggcaggc tgggggttca gggctggacg ccggcgagca
     8461 gcggcacgaa cacgacggcg ccaagtgaat gggtttcgta gccgatcgcc ttgcgctcga
     8521 cgatcagcag ttcctgcacg ccgccggccg gaccgagcgg cgccaccagc cgcccgccgg
     8581 tcgcgagctg ctccggcagc gccggcggca gccgttcggc cgccgcggtg accagaatgc
     8641 cgtcatacgg accgcatccg ggccagccga tgctgccgtc ggcgtgtttc agatgcacgt
     8701 tgcgcaaccc cagcgccgcg agccgctcac gcgcgcgcag cgacaggcgc tcgatgcgtt
     8761 cgacgctgta gacctgcccg accagcgggg cgagcacggc actctgataa cccgaaccgg
     8821 tgccgatctc cagcacccgc agcggcagcg cctcgcgccc ggcgagcagc gcctgcgtca
     8881 tgcgcgcgac gatgtacggc tgcgagatgg tctggccgag gccgatcggc agcgcggtgt
     8941 cctcataggc gcggctggcc agcgcctcct caatgaacag gtggcgcggc gtctcccgca
     9001 tcaccgccag cacgcgctca tcgtcgatgc cctgctcgcg cagccgcgcg accagccggg
     9061 tgcgggtgcg ggccgaggtc atgccgatgc cgcggaaggc gtccgggcgg ctcacggctg
     9121 cgtccacggg cgagcggcca tggccgggat caagccggcg catccagcca gcgtgcgacc
     9181 gtatcgagcg cggcatggcg cgtcaggtcg acctggatcg gcgtcaccga cacgcagtgc
     9241 tcgcggatcg cctggaagtc ggtaccgggt ccggcgtcgc tctcggcgcc ggccggaccg
     9301 atccaccaga tcggccggcc gcgcggatcg cgggtgcaga ccgccggctc ggccttgtgc
     9361 cggtgaccga gccgcgtgac ctgccagccg cgcagctcgc tccacggcac ggccggcacg
     9421 ttgacgttca ggatggtgtc ggccggcagc ggctggcggc acagccgccc gaccagatgc
     9481 agcgctacgc gcgcggccgt gtcgagatga tcgggttcgt aggcgcacag cgacacggcc
     9541 atcgccggcc ggccgaggaa gcgcccctcc atcgccgcag cgaccgtacc cgaatacagc
     9601 acgtcatcgc cgagattggc cccggcgttg atgccggaga cgaccatgtc cggctcgtgc
     9661 tccatcaggc cggtcagcgc cacgtgcacg cagtcggtcg gcgtgccgtc gaggctccag
     9721 acaccatcgg gatgacgaac gacgctgagc ggccggtcga gcgtcaggga attgctggcc
     9781 ccggagcggt tgcggtccgg cgcggccacg gtgatctcgg cgattccggc gagcgcctgc
     9841 gccagcgccc ggatgccggg tgccagatat ccgtcgtcgt tgctgacgag tatcctcatc
     9901 gaccgctcac cccgtccgcg cgccgcagcc ctgccccatg tcacgcagcc ttgccggcgg
     9961 cgcaaaccgg ccggtatcaa agacacatcg ccccggcaaa gtcaaaaacg cccgcccgat
    10021 gcctgccgac cgccctgaac cacccgccga cgaggtcgaa gccttccgtc aggccatcgg
    10081 cccgatcgag ccgcaccgct gcgatctcgt cgagccctgg cgcgcgccac gcccgccgct
    10141 gccgctgcag aagctcgccg acgagaagca ggtgctgcgc gacatggtct cggaccactt
    10201 cgagccggcc gatctcgaaa ccggcgagca cctgagctac agccgcgaag gcgtgcagga
    10261 tggcgtgctg cgcaagctca agcgcggtca gtacagcctc ggcgcccagc tcgacctgca
    10321 cggcctgacc tgggccgagg cgcgcgtgac cgtgcatgcg ttcctgagcc tcgtgcagcg
    10381 cgaccgcgtg cattgcgtgc gcatcgtgca tggcaagggc aaccgctcgc gccacctcgg
    10441 gccggtgctg aaacagaagc tgaacgtcta cctgcggctg cgcgacgacg tgctggcctt
    10501 ctgctcggcc cggcccgacg acggcggcac cggcgcgctg tacgtgctgc tgcgcagccg
    10561 ctgagacccg gggctacggc attcgtaaca gcgctgcgtc gccggccggc tgcgaggtgc
    10621 cgcgcagacc gtcgaacacg gcgatgagcc ggcgcgcctg ttcgtcgatc gaatagcgct
    10681 gcagcacgct cgcccgggcc gcagcgccga cgctgtgccg gtgctgcgga tcgcgcagcg
    10741 cgtcgagggc tgccagccag tcggcgcgcg agtgggcgat gtagccgttg acaccctgct
    10801 cgatgatgtc gagatagctc ggcaccgggc tcgcgaccac ggccagtccg gccgccatct
    10861 tcagcgtcag ccggttctcg gacttgacct gccagtacga gacctgccgg cccggcagcg
    10921 ggtccggacg catgtcgacc ggaatgatgc cgatctcggc gcctgcgaga tcctcgtaga
    10981 cccgctgcgg ctgccaggcc cgcgccgtga aggcgtagcc gcgcagccgc tccaggcgtt
    11041 cgccgggcgg cgcgtggcgg acgcggcgca ggcgcgcgcg cagcgcctgc agcggggtct
    11101 cgcgctccgg atactggccg acgacggtca gcgccagcca gcgcggcggc cgtccgagca
    11161 ccggcacggt ttcgggcgag cccgaggtca cgaggatcgc gcgcagccgg ccgtcgccgg
    11221 cggcgatgtc ttcccggcac cggacctccg ggtgctcgat gccgtcgtga acgacgtgga
    11281 tgcgcgcctg cagttccggg gcgtgctgct gcttcaggta gtcggtgacg atgaccgtcg
    11341 cgtcggtcag acgcaccatg tcatcgtcga tgcggtcgca gacaccgtag acggtgcgca
    11401 ccccggcctg gcgcagggtt tcgatctcgg cgcgcacgcc ggggccgtgc accttctgga
    11461 agtaggcgat gtcgatgccg tcggcgatca ggcgcgccgc cagcccgctc acgtccggac
    11521 gctcggtcgg ctgcggcggt gcgaaggcga tccgggggtc gtagccggcc gcacgcagcg
    11581 ccggcagcac gttgaacacc gagatgcgcg tgctcggcag cggtgccgcc gcatccgagc
    11641 acagcacgaa accgatccgc ctgcccgaag cactcatcgc gtcaggacat cgggaccgtt
    11701 tcgggcagcg ccgccggacg gcgcgcacgc aggctgaact ggtaggtcag gaactcgcgc
    11761 accggcgccc agcgctgcag cgcgtcgggc aggcggttca gcatgcggtt caggaaaccg
    11821 cccccgcgat ccccggcacg cagcacgaag ccctcggcgt cgatctgcag accggtatcg
    11881 gcgagcaggc tgcggccgtc ggcgatcgtg aaccagcgca ggtgggtcgc atcgaagatg
    11941 ccccggtcac ggcgcggaaa ccggccatga ccgtagatgc tcgcgagcgc cgagacgtgc
    12001 cggatgttcg gcaggctgag cacgatgcag ccgccggcat cgagacgctc cagcgcgccg
    12061 cgcaggtggc gctgcggatc gacgaggtgt tcgagcacgt cggcgaacac gatgcagtcg
    12121 aagcgctcgt cgccgagacc gtccgaccag tcgaaggcat cgagatcggc ctgttcgacc
    12181 cgatcgagca gcagcgcggc ctgacgggcg aagccggcgt cgagttcgat gccgacgacc
    12241 cgccggcccg ccacctcggc gcgcaggctg gcaccgagcg caccgttgct gcagccgaca
    12301 tcaaggatgc gcatggcctc gcgcggcacc agggcggaaa cgtcagcgcg cggcgtcgta
    12361 taggccgact cgcgattcag aagggttccc gcggcggcgg tcatcatctg cgtggattct
    12421 ccggggctga caggaccggt cgcaccggta cgccgggttt atagcatgtc gtcctgcgcc
    12481 tccggattac cgaccagttc atgcagcacc gtcgtcgcgt aggcgccggc aggcaatctg
    12541 aagtcgagcc gcacggcatc cccgccgatc gcctcgccat gcagttgcgc cggcaccagc
    12601 cgcagcgcgc gccgctcctg cttcagccct tcggccgcga gtcccgcgca gagctgcgcc
    12661 tcggccgcgg ccagcgccgt ctcgccggcc agcgccgccg ccgtcgccag cgattcgccg
    12721 gcgccccaca gcgggccgct cggatgcagg tcgtgccggg ccaggcgctc caggatggtt
    12781 gcgtccggct gcgggcacgc gaacaccgaa tggctgccgt cgagcatcag cacctcgccc
    12841 ggcagcgcct gatcccagct gccatcgctg atccgtgcgg cgagatagcg gttgaacagg
    12901 tgcgagcggg ccgccgacag gtacaggccg cgcttgtgac ggtcgcgctc gcgcagcgtg
    12961 ccggcgagca gccgccgcgc gcgttcgaga ttgccgccgt cgcggccgaa gcgctgttcg
    13021 ccgaaccagt tcggcacgcc gcgggcggcg atggccggca ggcgatcgag cacggcggca
    13081 ggcgggccgt cgagctcgcg cacgacgatg cggaagcggt tgccggccag cgcaccgcga
    13141 cgcagcttgc ggctgttgcg ggtgacggcc agcacctcga cgccgtcgat gccgcggctg
    13201 ccggtgaagc cgcacggcat cggcgcctcg cgccccggca tctgcaccga gaaccactgc
    13261 tcggtgacgg cgtggcggtc cttgagcccg gcgtagctga cggcgtcggc gcgcacctcg
    13321 cagacttcgg cgagccggcg cgccacccag tcggtgttct ggccgctctt gcgcacgtac
    13381 aggaacacgt gctcaccgct gccgtccggc tgccagccaa gctcctcgat gacgatgaaa
    13441 tcctcgggcg cgcagcggat gcggccgcag agcggcggcc ggccgtgggc atgcggcagt
    13501 tcgtcgacag tggtgttcac agcaccgccg cctcgaccag cagcaccacg gcctcgaccg
    13561 cgatgccctc gccgcggccg gtgaaaccga gccgttcggt ggtcgtcgcc ttgacgttga
    13621 ccgcatcgcg gttgacgccg agatcggcgg cgatgtgctc gcgcatggcc tcgatgtgcg
    13681 ccgccatgcg cggggcctgg gcgatgaggg tcgcatcgac gttgccgacc gcgtagccct
    13741 cggtgcggat cagttcgacg acgcgatgca gcagccggcg gctgtcgatg ccgcggtaac
    13801 gctcgtcgct gtccgggaag tgccggccga tgtcgccgag cgcagcggct ccgagcagcg
    13861 catcgcagag cgcgtgcagc agcacgtcgc cgtcggaatg ggcgatgagc ccgtgcgtgt
    13921 ggggaatgcg cacgccgccg agcgtgacgt gatcacccgg tccgaaggcg tgaacgtcga
    13981 aaccgtgacc gatgcgcatc gcgggttcct cgggctggcc gctacggggc gcgcagcgta
    14041 gcgcgccttg cgagccgcga ccaagcccgc gcgccccgtg cggtcgaggc ggactcagcg
    14101 ccagccgccc tcgacccaga tccagccgga aggccgtgac tcccagtgac ccgcctgcca
    14161 gcgggcacgc ggctgcgggc ggttctccca gtggccgggc tgccagtccc agccgttgcg
    14221 ccagacccag cgccccggca cccagtcggc gttcggcgaa ggccggtacg gccgggcttc
    14281 gcgacgcggt ggcggaggcc gttcgcgcac gtattcgacg cgctcctcga cgacgacggg
    14341 gcgtggtgcc gggcgctcga cgacggccgg ccccggacgc acgaccgtgg tacagccggc
    14401 cagcgccaga acggacagcc cgcccgccag catgagcgga cgcaggttca gggtgatttt
    14461 catgatggct cccgtggaag gtgtgacgcg cgtgcgtcgc aggcttcgac aggctcccgg
    14521 gcggaagtgc cggccttcag gggcgtccgg cggccaggaa gaacgcggcg agcgccagat
    14581 cctcgggccg cgtgatcttc aggttgtcgg cgcggccctc gaccagccgc ggccgcaggc
    14641 cggcgcgctc gatcgccgaa gcctcgtcgg tgatcaccgc tccggccgcg gcggccgctt
    14701 ccagcgcatc gcgcagccgt ccgtagcgga acatctgcgg cgtcagcgca tgccagagcc
    14761 gctcgcgctc gacggtgtgc tcgacgcagc catcggccgc ggcgcgcttc atcgtgtcgc
    14821 gcaccggcac cgccagcagg ccgccgagcg gatcgtcggc gagggcggtg aacagccgtg
    14881 cgaggtcttc ggccgccaga cacgggcgtg ccgcgtcgtg gaccagcgcc cagtcatcgg
    14941 tcccagccag cccttcgagc gcacgcaaac cgttcagcac cgaatcggcg cgctcggtgc
    15001 cgccgtcgac gcgccggatg cgtgccggat cggagagctt cagtgccggc caccagccgt
    15061 cctcggggcc gagcgcgacg acgatgcgct cgatgcgcgg ctcggcgagc aggcgttcga
    15121 gcgtgtgttc gagcacgcag cggccggcga gcggcaggta ctgtttcggg atcgccgcgc
    15181 ccatgcgcct gccgacgccg gcagccggaa cgatggcgaa gcagcgcgcc atcagtcggg
    15241 ccggccgctg tcgggcgccg gtttcgaagc cggcgggcgc gggacggccg gtttcgcggc
    15301 gggtttcgca tcgggcttcg gagcggccgg cgccgcggct gcgggcgcgg acggggcctg
    15361 cggccgcgcg tggatcgcgg tgccgggttt ggtcagcgcc gagggcggcg aactcggggc
    15421 ttcgtcgagg atgcgataga aggtctcgtc gccgcgcacc atgccgagtt cggtgcgggc
    15481 ccgctcctcg atcgcggcat tgccctgctt gagatcgcgg acttcggctt ccagcgactt
    15541 gttgcgatcg gcgagttcgg tgttttcgga tttctggtcg gcgaccgcac gttcaagcgt
    15601 gcgcatctgg cgcagcccgc ggtcgtcggt catccacagc aggtaattca gcccgagcag
    15661 cagcgcgatc agggcagcga ggaagaggcg cattcggggc cgatcgaacc tgcaaaacgg
    15721 ggggaaatca ggcggtgcgc acgcgcggcg gcgtgcgcac cggagagccg cgggctcagc
    15781 ccagcttcag cttgcgcggg aaggccttgc ggccggcgta gacggcatcg gcaccgagct
    15841 gtgactcgat gaccagcagc cggttgtact tggcgacgcg gtcggagcgg ctcagcgagc
    15901 cggtcttgat ctgacgcgcc gtggtcgcga ccgcgaggtc ggcaatggtc gtgtcctcgg
    15961 tctcgccgga gcggtgcgag ctgatcgagc tgtagccggc ctcatcggcc atgcggatcg
    16021 cgttcagcgt ctcggtcagc gtgccgatct ggttgacctt gatcaggatc gagttggcga
    16081 tgccttcgcg gatgccgcgc tgcaggatct cggtgttggt cacgaacagg tcgtcaccga
    16141 cgagctggat gcgctcgccg agcagccggg tcagttcggc ccagccggcc cagtcggatt
    16201 catccatgcc gtcctcgatc gaaatgatcg gatagcggcc ggcgagatcg gcgagcacgc
    16261 cggcccattc gagcgacgaa tacgccttgc cgtcggcttc gagcgtgtag cggccgttcc
    16321 tgtagaactc ggagctggcg cagtcgagtg cgagcatcac gtcgtcgccg gcgcggtagc
    16381 cggccttgcc gatcgcttcc atgaccactt cgagcgcggc ctcgttcgac ggcaggttcg
    16441 gcgcgaagcc tccctcgtcg ccgacgccgg tcgccaggcc gcggccgttc aggaccttct
    16501 tcagcgcgtg gaagatctcg gcgccgcaac gcagcgcttc cgagaagctc ggcgcaccga
    16561 ccggcatgac catgaattcc tggatgtcga ccgagttgtc ggcatgcgcg ccgccgttga
    16621 ggatgttcat catcggcgtc ggcagcacgt aggggccatc cgggttgatg tgccggtaca
    16681 gcggcacgcg cttctcgctg gccgcggcat gcgcgaccgc gagcgacacg ccgagcagcg
    16741 cgttggcgcc caggcgcccc ttgttggcgg tgccgtcgag ctcgatcagc cgcgcatcga
    16801 gcgccgcctg atcggccgcc tccatgcccc gcaccgcctt caggatctcg ccgttgacgt
    16861 ggccgacggc cttcagcacg cccttgccga ggtagcgccc ggcatcgccg tcgcgcagct
    16921 cgatcgcctc gcgcgtgccg gtcgaggccc ccgagggcac ggcggccgtg ccgcgcgcgc
    16981 cgctcgcgag ctcgacctcg acctgcacgg tcgggttgcc gcgcgagtcg aggatttcgc
    17041 ggccgatcag ggattgaatc gcggacatcg tcacttctcc tgctgtcttg tcggaaacac
    17101 cgggttcgcg gcggttgcgc gtcgcccggc cggggatggt gatatcgggg gaaatcctga
    17161 agcctgccgg gaaatcagcc cggatcgttt tcgtcgaagc cgcggcgctt caccagcgca
    17221 tcgagttcga ccagggtttc gagcaggccc tgcatgcgtc cgagcggcca ggcattcggg
    17281 ccgtcggaca gcgcctgttc gggctgcgga tgggtttcca tgaacagccc ggccacgccg
    17341 acggcgaccg cggcgcgggc gagcaccggc acgtgctggc gctggccgcc ggaactcgtg
    17401 ccctgcccgc ccggcagctg caccgaatgc gtggcgtcga acaccaccgg acagccggtg
    17461 tcgcgcatca cggccagcgc gcgcgggtcg gaaaccaggt tgttgtagcc gaaggagacg
    17521 ccgcgctcgc agaccatgat ctgcgtgttg ccggtcgcga gcgccttgtc ggcgacgttc
    17581 ttcatgtccc agggcgcgag gaactggccc ttcttgatgt tgaccgggcg cccggcgcgc
    17641 gcgaccgcct ggatgaagtt ggtctggcgg cacaggaagg ccggcgtctg cagcacgtcg
    17701 acgacggcgg cgacctcggc gagcggcgtg tcctcgtgca cgtcggtcag caccggcacg
    17761 tcgagctcgc gccggacggc ttcgaggatc gcgagccccg cttccagccc cggcccgcgg
    17821 tagctcgcgt gcgagctgcg gttggccttg tcgaagctcg acttgtagat gaagggcacg
    17881 ccgagcgcgg cgcacatctc cttgaggcgg ccggcggtct cgaaggccag ctcgcgcgat
    17941 tcgatcacgc aggggccggc gatcaggaac agcggccggt cgaggccgac ctcgaatccg
    18001 cagagcttca tgcgagcttg ccctcgcggt gcgcgaccgc cgcctcgacg aaggcgcgga
    18061 acagcgggtg gccgtagcgc ggcgtcgatg tgaactcggg atggaactgg caggccagga
    18121 accacgggtg atcgggcagc tcgatcatct cgaccaggtt gccgtcctgc gagaagcccg
    18181 agaagcgcag gccggatttc tccagcaccg gcacgtagcc gtcgttgaac tcgtagcggt
    18241 ggcgatggcg ctcgacgatc acgtccttgc cgtagacgcg gtgcgccagc gtgccctcga
    18301 ccagccgtgc cggctgggcg ccgaggcgca tcgtgccgcc gaggtcggat tcctcggagc
    18361 gacgctcgac cgcaccttcc cggttcgtcc attcggtgat cagcgcgatg accggatgcg
    18421 tcgcgttctt gcggaactcg gtcgaatgcg cgccttcgag gccggcgacg ttgcgcgcgt
    18481 actcgatgac ggcgacctgc atgccgaggc agatgccgag atacggaatg cgctgctcgc
    18541 gcgcatggcg caccgcggcg atcttgcctt cgacgccgcg ctcgccgaag ccgccgggca
    18601 ccaggatcgc gtgcatgccg gccaggcagc cggtgccctc gcgttcaagc tgctcggagt
    18661 cgatgtagcg gatcgcgacc ttgtggccgg tgtggaagcc ggcgtggaac agcgcctcgt
    18721 tcagcgactt gtaggcatcg gcgagatcga cgtacttgcc gaccatcgca atctcgatcg
    18781 tgtcgccgcg cgccgcctcg atctgcccga ccacgcgcgt ccattcgtcg agattcgccg
    18841 ggtcggcgcg caggccgagc ttctggcaga ccacatcgtc gagcccctgc tggtgcagca
    18901 gcagcgggat gcggtagatc gtgtcggcgt cgacggcgct gaacaccgcc ttggcctcga
    18961 cattcgtgaa cagcgcgatc ttgcgccgct cctcggccgg gatttcgtgc tcgcagcggc
    19021 agagcaggat gtcgggctgg atgccgatcg agcgcagttc cttgaccgag tgctgcgtcg
    19081 gcttggtctt cagctccccg gccgtggcga tgtacggcag cagcgtcagg tgcatgaaca
    19141 gcgcacgctc gcggccgagt tcgacgccga gctggcggat cgcttcgagg aagggcagcg
    19201 actcgatgtc gccgaccgtg ccgccgatct cgaccatgac gatgtcggcg ttgccggcac
    19261 cgaggcgcac gcagcgcttg atctcgtcgg tgatgtgcgg gatcacctgc acggtgccgc
    19321 cgaggtagtc gccgcggcgt tccttgcgga tgacgttctc gtagatgcgg ccggtcgtga
    19381 agttgttgcg ccgcgtggcg gtcatgcgca cgaagcgctc gtagtggccg agatcgagat
    19441 cggtctccgc gccgtcctcg gtcacgtaca cctcgccgtg ctgaaacggg ctcatcgtgc
    19501 ccggatcgac gttgatgtac ggatcgagct tgatcatcgt gacctgcagc ccgcgcgctt
    19561 cgaggatcgc ggcaagcgag gccgcggcga tgcccttgcc cagcgaggag acgacaccgc
    19621 cggtgacgaa gatgaatcgg gtcatgggaa gtgttccagg cccgcttcgc gggcaccgag
    19681 aggggcggca acgaagcgcg gcatggtact ggatggcgcc tgcgtttgcc acgcgggcac
    19741 ccgacccggt gcatcgggtc cggccagcca gccctcggcg atgcccaggc cggcgaccgc
    19801 gaccagttcc tcgtcgatat aaaggagggg cagccgggcg cgcagccacg gtgcgatgcc
    19861 ggcggcctgc agcagcttct tgagctcctg cgcatgccgg cggcccgcgg gccggaagcg
    19921 ctcgccgccc tggcgcagcc gcagcgtcac gcgccggccg gcgagccgcg cggcatcgag
    19981 gccggccccg cttgccggct gccaaaccag cgtgccgagg ccggcgatgc tgatttcccc
    20041 gccctccggc ggccagtcga gacaaagcgc atccggcagc gccggcagcg gcgcgaacac
    20101 gtgcagccgc tcgcggaagc ggtgcatctc ggcgcccggc cagcacacgc agggctcggc
    20161 atcgcggcgc gccggcagca ggtcacgcag cacggtgtcg aggcgtgccg ccggcgggat
    20221 gggcagctcc gggtccagct gagggatgtg
//
LOCUS       DAOQIE010000023        20115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1206_length_20115_cov_8.066650, whole genome shotgun sequence.
ACCESSION   DAOQIE010000023 DAOQIE010000000
VERSION     DAOQIE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 20115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20115
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_20115_cov_8.066650"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2358
                     /gene="alaS"
                     /locus_tag="PK144_02790"
     CDS             <1..2358
                     /gene="alaS"
                     /locus_tag="PK144_02790"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNF64684.1"
                     /translation="MLGNFSFGDYFKRDAISYAWEFLTSPQWLGLPKDKLMVTVYASD
                     DEAFDIWNKEVGVPADKIVRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGPEIA
                     GGPPGSPDEDGDRYIEIWNIVFMQFNRDDSGTLHPLPKPSVDTGMGLERLAAVLQHVH
                     SNYEIDLFRRLIAAAAEATGARDLGLPSLKVIADHIRSTAFLIVDGVVPSNEGRGYVL
                     RRIMRRAIRHGYKLGQKQPFFHRLVGPLVAEMGEAYPELAAAEGFVVRTVLAEEERFA
                     ETLETGMRLLDADLARLGGEIIPGETVFQLYDTHGFPVDLTADIARERGLSLDMAGFE
                     RAMDAQRARSRASSQFGSYHSDALAVGSGSEFHGYESLTATGTVTALFRDGQPVEALA
                     AGEDGIVVLDRTPFYAESGGQVGDSGVLRAGEAVFEVADCRKQGEGAFLHIGRLVSGS
                     LSTGATVEAEVDAARRAAIRLNHSATHLLHAALRQVLGTHVQQKGSLVDADKLRFDFS
                     HPQALSPEELDRIEALVNAEIRANHAVETRIMGLDAAKAAGAMALFGEKYTDEVRVLS
                     MGGFSTELCGGTHASRTGDIGLFKIVAEGGVAAGVRRIEAVTGANAWVWLRELDARVK
                     AIAALVRGDAGSVETKVRGLVDRVRGLEKDVEQLKAKLASAQGGDLAAQAVEVAGVRV
                     LAARLDGADARTLRETIDQLKNKLHTAAVVLATVEDGKVRLVAGVTADQTDRLKAGEL
                     VNHVALQVGGKGGGRADLAQAGGTDPAALDAALAGVPAWVAGQIG"
     gene            2427..3653
                     /locus_tag="PK144_02795"
     CDS             2427..3653
                     /locus_tag="PK144_02795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003193595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNF64685.1"
                     /translation="MALIVQKYGGTSVGSVDRIENVAKKVIGYRGQGHDVVVVVSAMS
                     GETDRLIGLAKAIDPRPNPRELDVLISTGEQVTIALLCMALEKHGQPARSYTGAQVRI
                     LTDSAFTKARILAIDGENIRSDLAQGRVVVVAGFQGVDEDNNITTLGRGGSDTTGVAL
                     AAALKADECQIFTDVDGVYTTDPRIEPRARRLDRITFEEMLEMASLGSKVLQIRSVEF
                     AGKYNVPLRVLSTFQEGNGTLITLEDENMEAALISGIAFSKDEAQITVTGVPDRPGIA
                     FGILGAVSDANIEVDMIVQNIGRDGTTDFTFTVHRNDFERALEIVQGKAVELGAREIN
                     GDRKIVKVSLVGVGMRSHAGIASRMFQALAREGINIRMISTSEIKISVVIDEKYLELG
                     VRTLHEAFQLDQAPAA"
     gene            3839..4036
                     /gene="csrA"
                     /locus_tag="PK144_02800"
     CDS             3839..4036
                     /gene="csrA"
                     /locus_tag="PK144_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNF64686.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVRGNQVRIGVNAPKDIAVHR
                     EEIYERIKREQEGGADRGEHE"
     gene            4120..4213
                     /locus_tag="PK144_02805"
     tRNA            4120..4213
                     /locus_tag="PK144_02805"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4154..4156,aa:Ser,seq:gct)
     gene            4290..4366
                     /locus_tag="PK144_02810"
     tRNA            4290..4366
                     /locus_tag="PK144_02810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4324..4326,aa:Arg,seq:acg)
     gene            4539..7355
                     /gene="prsT"
                     /locus_tag="PK144_02815"
     CDS             4539..7355
                     /gene="prsT"
                     /locus_tag="PK144_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNF64687.1"
                     /translation="MSGMRHGGAALVLLALLATMPLPAPAAKSEGASTYYEDALIRFD
                     RNDLDGAEIQIKNALQQNRDHLASHILLGRIYLLKGRAEAAVFELQTANRLGADRTTT
                     DPYRARAYLQLGRYKDLIDTVSGDGLAPRTRAEVLAARGSALLELGQLFDAEQAFASA
                     KDADPASPAGWSGMTVLRLRQGALVQAEQEAQHAVDLGPGDAEAWNALASVMHQSGKD
                     DEALAAYARALEADPRMLAARLARAGLLLDLDRLDEAGQDIESLRKEYPVEPRGMLLR
                     ALWLQRRGETDAARAALADTGAVLKAVPRETMERFPALLLVSGMTHSALGELERARDD
                     LARYLELAPDNPGARKQLGAVYVRLNEPTQAVAVLEPARLRVPDDPRILALLGIAYSM
                     KGSHLVATELLEKASALSGGAADIRTDLAASQLRIGRQEQALDELATVYDKDPAQTRA
                     GVLLAGLHLQRGEAAKAVDILQQLVGQEPRNLTFLNLLASALLASGRQPEARTMLERA
                     IEIDPAFLPAQLGLARLDILARKFDQARARLQARLQATPENVQLLVEMARVDAITGAR
                     DTAIQRLERARSLSADAIEPRQFLIDLYLRRNEGNQALEVAEELVARHPDNLASLAAL
                     GTTQLVLGKGPLAQVTFRRMVKFAGFDPIALMRIARLQEQAGALNDSAFTLEQAVHAE
                     PNRPELLAALAGAYLVTGRLEEAEEQARRLQQLDPASPVSQRLLAEALLRRNKPAEAA
                     AMFRQAMAKTPSAELVIGAFRALLADDRAADGIRMLEDWVAQHPDDRNVRTALAEGYM
                     RAERWKEALPLYEALLAVQPEQPQVLNNLAYIALQTGDEAKALDYARRAAALAPQDPA
                     ITDTLGWILVRAGRAQEALQYLREAQSRDAGNPEIRYHLGVALMQLGRKAEARRQFEQ
                     AIAVKLAYPGIEEARRLYRETAGP"
     gene            7504..8328
                     /locus_tag="PK144_02820"
     CDS             7504..8328
                     /locus_tag="PK144_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64688.1"
                     /translation="MQDRNGFLHGDSNRGRAMDIRRAALFAGMASMLLAVTAGAGVSY
                     TFATDCAAGTGASLSCANGRLGVSAWSNTVGTANRYIENATVVNYGSYGLGITNADSS
                     LPSGADAGEGTSPEHAIDNNGRYDALLLDFGAGNQFGLTGFRNGWYSTDSDMSFLAYT
                     GSGDALAGLASRTLSFGDAASDPASLLNNGWTRVSDYADAGTVARSLTAGVASRYWLV
                     SAYVPMSATYSLSVGNDYVKLAAIEGSYLRQVPLPATWWLLVPGLLWLYRLRSRRA"
     gene            complement(8479..8555)
                     /locus_tag="PK144_02825"
     tRNA            complement(8479..8555)
                     /locus_tag="PK144_02825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8519..8521),aa:Pro,seq:cgg)
     gene            complement(8574..9542)
                     /gene="ispB"
                     /locus_tag="PK144_02830"
     CDS             complement(8574..9542)
                     /gene="ispB"
                     /locus_tag="PK144_02830"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HNF64689.1"
                     /translation="MNISEVRALAEADMQAVDRLIQRELHSDVALVNQLGAYIINSGG
                     KRMRPVLTVLAARACGYAGNDHIDLAAIVEFIHTATLLHDDVVDASELRRGRETANAI
                     WGNEASVLVGDFLYSRAFQMMVRLDSMRVMRILADTTNRIAEGEVLQLLNCHDPDVDE
                     ARYFEVIYSKTAKLFEAAGRLGAVVAGHPAGIEQALAEYGMRLGTAFQLVDDLLDYSA
                     SSDEIGKNVGDDLAEGKPTLPLIYAMRHGSAAQASMIRQAIENGDRNQIGVITEAIAS
                     TGAVAYTARLARQEADAAIEALRILPESIQKQALIALADYSVSRSH"
     gene            9745..10059
                     /gene="rplU"
                     /locus_tag="PK144_02835"
     CDS             9745..10059
                     /gene="rplU"
                     /locus_tag="PK144_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNF64690.1"
                     /translation="MYAVIKTGGKQYRVAEGQTLKVEKLDVEEGASVELAEVLLVSDG
                     ETVTVGAPTVGGAIVTATVQSHGRAKKVEIVKFRRRKHHRKQMGHRQHYTELKITGIR
                     VG"
     gene            10077..10334
                     /gene="rpmA"
                     /locus_tag="PK144_02840"
     CDS             10077..10334
                     /gene="rpmA"
                     /locus_tag="PK144_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNF64691.1"
                     /translation="MAHKKAGGSSRNGRDSESKRLGVKMYGGQLCIAGNILVRQRGTQ
                     FHPGVNVGLGVDHTLFAKADGRVVFETKGPDSRKYVSVVPA"
     gene            10426..11493
                     /gene="obgE"
                     /locus_tag="PK144_02845"
     CDS             10426..11493
                     /gene="obgE"
                     /locus_tag="PK144_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNF64692.1"
                     /translation="MKFVDEAAIRVMAGDGGNGCVSFRRERFIPFGGPDGGDGGDGGS
                     VWLVADADLNTLVDFRFTRTFKAGRGENGGGANRTGAAGADLDVPVPVGTTAIDADTG
                     ETIGDLTAPGQRLLVAQGGFHGIGNARYKSSTNRAPRQSKPGTTGEVRALQLELKVLA
                     DVGLLGMPNAGKSTLIRAVSSARPKVADYPFTTLHPNLGVVRVGPLKSFVMADIPGLI
                     EGAAEGAGLGVQFLKHLARTSLLLHLVDLSPYSDSGDPVADVRTIEAELAKYSAELAG
                     RERWLVLNKLDLLPEDEREARAQAVVEALGWSGPVFRIAAIGAEGTEPLCWRIMERLD
                     AARAARPADAALPIDSEGEGA"
     gene            11493..12620
                     /gene="proB"
                     /locus_tag="PK144_02850"
     CDS             11493..12620
                     /gene="proB"
                     /locus_tag="PK144_02850"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNF64693.1"
                     /translation="MAVSRETLGQARRWVVKIGSAMITDEGRGLDAAAIEAWVAQLAE
                     LHAEGRDVIIVSSGAVAEGMRRLGWRARPKALAELQAAAAVGQMGLVQGWEAAFQKHG
                     IRTAQILLTHEDVSDRQRYLNARNTLRTLTRLRVVPVINENDTVAFEEIRFGDNDTLG
                     ALVANLVEAELYVILTDQHGMYDRDPRAHADARFLSEVSAGDPALERMAGGGAGTLGR
                     GGMLTKVRAAAKAARSGTATLLAWGREPEVLRRVARGEPIGTLLRPQAGPVAARKQWL
                     AVQLQVRGWLHLDAGAVQALKLGGRSLLAVGVRGVEGEFQRGELVACVGPDGVEVARG
                     LVNYDASESVRIMGKPTEAIESVLGYVDAPALIHRDDLVLV"
     gene            complement(12759..13022)
                     /gene="rpsT"
                     /locus_tag="PK144_02855"
     CDS             complement(12759..13022)
                     /gene="rpsT"
                     /locus_tag="PK144_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNF64694.1"
                     /translation="MANTAQAKKRVRQAEKARQHNASLRSRFRTYVKKVIAAIRAGDK
                     SQAEAAFRSATSVIDSTARKGLIHVNKAARHKSRLSAHIKAMA"
     gene            13245..14786
                     /gene="murJ"
                     /locus_tag="PK144_02860"
     CDS             13245..14786
                     /gene="murJ"
                     /locus_tag="PK144_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNF64695.1"
                     /translation="MSSKSLMRSTGVVSALTFLSRLLGFVRDMVYAGLFGAGAGMDAF
                     NVAFRIPNLFRRLVGEGAFSQAFVPVFSQYRTQRSPEELRLLVDQVAGTLGTVLLALT
                     AFGMIGAPLLVWAFASGYGDEPYKFALTEQMLRITFPYVLCICLTAFAGGILNSCGHF
                     AVPAVTPVLLNVTMIAAALLAAPHFAEPALALAWGVALAGVLQLLFQWPALKRIGLVP
                     RPRLAWRAEGVRRIMMLMVPALIGSSAAQINLLINTQLASTLPTGSVSWLYYSDRLVE
                     FTLGIFGAALGTVILPKLSRQHAAAEGEGFAHTLDWGLRWSLLIGTPATVALAILAGP
                     LLATLFMRGEFTATDVEMTTRSLMTYGSGLVAYMLVKVLAPGFYARQDTKTPVRFGLI
                     SIGANIGFQMLLIAPLAHAGLALSTALAAFVNVALLYWQLRRDGVYRPRPGWPAFWLR
                     MLAANAAMAGFLVWAAGDLSGWIAADTLTRVLRLAWIVPAGAGVYAAVALGLGVRLSQ
                     LRAPA"
     gene            14883..15821
                     /gene="ribF"
                     /locus_tag="PK144_02865"
     CDS             14883..15821
                     /gene="ribF"
                     /locus_tag="PK144_02865"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNF64696.1"
                     /translation="MELIRGTHNLRPRHRGCVATIGNFDGVHLGHRAVLDQLHARADA
                     LGLPAVVITFEPYPQEYFARSQPPARLTRLREKLVALEAAGVQRVLVLAFNARLAAMR
                     AGDFVQHLLVDGLGVRFLVVGDDFRFGHGREGDYALLERCGAAAGFEVAHTATHLIDG
                     VRVSSTRIRERLAAGELAEAARLLGRPYAVHGRVAHGQQLGRTIGFPTANVHLRRRAT
                     PLGGVYAVVLRSPGLPPLAGVANLGRRPTVGGTREQLEVHCFDFDGDLYRRRVQVEFR
                     ERLRPEQRFPSLDELKLQIARDAEAARAHFRARPAD"
     gene            15861..18704
                     /gene="ileS"
                     /locus_tag="PK144_02870"
     CDS             15861..18704
                     /gene="ileS"
                     /locus_tag="PK144_02870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNF64697.1"
                     /translation="MADYKDTLNLPDTAFPMKGNLPQREPEQLARWAGMNLYAQQRLA
                     CAGRPKFVLHDGPPYANGDIHIGHAVNKVLKDIIVKSRTLAGFDAPYVPGWDCHGLPI
                     EHAVEKKVGKVGVKVDARSFRQQCRAFAHGQVDRQREDFIRLGVLGDWQHPYLTMDFR
                     TEADIVRSLGRIVGNGHLLRGSKPVYWCLDCGSALAEAEVEYEDKDSLAIDVRFPVVE
                     AASVFARLHHAEGGDGKGPLSVVIWTTTPWTLPANRAVTLGAELEYVVVQAETAQGSE
                     RLLLAEGLLKDCMLRWGVERYQVLAYGKGADLEGLKLAHPFYEREVPVILGEHVTLEA
                     GTGCVHTAPAHGQDDYVVSSRYGLRIDDNPVGPDGRFLPETPLFGGLHVHGANERILD
                     VLKGHARLVHAEKLRHSYPHCWRHKTPIIFRATPQWFISMEQAGLRAQALAAVEQLRF
                     TPDWGRARLEGMIANRPDWCISRQRYWGVPIVLFTHRRSGELHPRWAELIEQVALKIE
                     QEGIDGWFSSTAEDWLGPEAADYEKVTDTLDVWYDSGTTHASVLERRPELGFPADLYL
                     EGSDQHRGWFQSSLLASVAMRGVAPYKGLLTHGFTVDQQGRKMSKSLGNVIAPQEVIK
                     ELGADVLRLWVAATDYRGEMTVSKEILRRMADSYRRIRNTARFLLANLNGFDPARDAV
                     EPARMLPLDRWVVDRAARLQAEVCSAYDDYLFHVIYQKVHNFCSVDLGSFYLDVIKDR
                     QYTGARDGLPRRSAQTALYHVLEALVRWIAPICSFTAEEIWKHMPGARAESIFLDTTW
                     YPGLFTLDADDAFGADYWARLIDVRGAVSKAIEDLRKGGGIGGSLEAEVDLYADESLR
                     TDLARLHDELRFVLITSQARVHPLDQAPGSAVLAPDGRLALVVARSEAQKCVRCWHLR
                     PDVGTHAAHPQLCGRCIENVDQAAAGGERRLYA"
     gene            18704..19177
                     /gene="lspA"
                     /locus_tag="PK144_02875"
     CDS             18704..19177
                     /gene="lspA"
                     /locus_tag="PK144_02875"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNF64698.1"
                     /translation="MARWLWLSVAVLALDQISKYAAETLLLLHRPVPVLPPVLDFMLA
                     YNTGAAFSFLAGAGGWQRWFFIVLAGAVSVALTVWIVRLHAHERRLAVALALVLGGAI
                     GNLVDRALWGHVIDFILVHFGDWPFPAFNLADSAITVGAALLVLDSLFPHRAPDA"
     gene            19190..>20115
                     /gene="ispH"
                     /locus_tag="PK144_02880"
                     /gene_synonym="lytB"
     CDS             19190..>20115
                     /gene="ispH"
                     /locus_tag="PK144_02880"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNF64699.1"
                     /translation="MQIRLANPRGFCAGVDRAIEIVERALDLFGAPIYVRHEIVHNRY
                     ICDKLRAEGAIFIEDLAEVPDGSTLIFSAHGVSRAIHDEAARRGLKVFDATCPLVTKV
                     HMEVVRYAKQGIGVVLIGHAGHPEVEGTMGHYDRSAGGAIHLVETVADVARLTVADPQ
                     RLAFVTQTTLSVDDTVGIIDALRARFPAIHGPRKDDICYATQNRQDAVKELAADCDVV
                     LVVGSPNSSNSNRLREIAQKLDVPAYLIDAAEDIDPDWLAGRQRVGVTAGASAPDVLV
                     QRVLQRLRDECGAEVQPDDGGIKENVVFALPR"
BASE COUNT         3127 a         6773 c         6978 g         3237 t
ORIGIN      
        1 atgctgggca acttcagctt tggcgactac ttcaagcgcg acgccatcag ctacgcctgg
       61 gagtttctca ccagcccgca atggctgggc ctgcccaaag acaagctgat ggtgacggtc
      121 tacgccagcg atgacgaggc gtttgacatc tggaacaaag aagtcggcgt gccggcggac
      181 aagattgtgc gcattggcga caacaagggc gcgccgtatg cctccgacaa cttctggcag
      241 atgggcgaca ccggtccctg cggcccgtgc accgagatct tctacgacca cggtccggag
      301 atcgccggcg gtccgcccgg ctcgccggac gaggacggcg accgctacat cgagatctgg
      361 aacatcgtgt tcatgcagtt caaccgcgat gattccggca cgctgcaccc gctgccgaag
      421 ccgtcggtcg acaccggcat ggggctggag cgcctcgcgg cggtgctgca gcacgtgcat
      481 tcgaactacg agatcgacct gttccggcgc ctgatcgccg cggccgccga ggccaccggc
      541 gcgcgtgatc tcggcctgcc gtcgctgaag gtcatcgccg atcacatccg ctcgacggcc
      601 ttcctgatcg tcgacggcgt cgtgccctcg aacgaaggcc gcggctacgt gctgcgccgg
      661 atcatgcgtc gggcgatccg tcacggctac aagctcggtc agaagcagcc gttcttccac
      721 cggctggtcg ggccgctggt cgccgagatg ggcgaggcct atccggagct ggcagcggcc
      781 gagggcttcg tcgtgcgcac cgtgctcgcc gaggaggaac gcttcgccga aaccctcgaa
      841 accggcatgc gcctgctcga tgccgacctg gcccggctcg gcggcgagat catccccggc
      901 gagaccgtgt tccagctcta cgacacgcat ggctttccgg tcgacctgac cgccgacatc
      961 gcccgcgagc gcggcctgtc gctggacatg gccggtttcg agcgcgcgat ggacgcgcag
     1021 cgcgcccgct cgcgcgcatc gagccagttc ggcagctacc acagcgatgc gctggcggtc
     1081 ggttcgggca gcgagttcca cggctatgag tcgctcacgg ccaccggcac ggtcacggcg
     1141 ctgtttcgcg acggccagcc ggtcgaggcg ctggcggccg gcgaggatgg catcgtcgtg
     1201 ctcgatcgca cgccgttcta cgccgaatcc ggcggtcagg tcggtgacag cggcgtgctg
     1261 cgcgccggcg aggccgtgtt cgaggttgcc gactgccgca agcagggcga gggtgccttc
     1321 ctgcacatcg gccggctcgt gagcggcagc ctgtcgacgg gcgcgacggt cgaggccgag
     1381 gtcgacgccg cacgccgcgc cgcgatccgc ctgaaccatt ccgccacgca cctgctgcat
     1441 gcggcgctgc gtcaggtgct cggcacacac gtgcagcaga agggctcgct ggtcgatgcc
     1501 gacaagctgc gcttcgactt ctcgcatccg caggcgctga gtcccgaaga actcgatcgc
     1561 atcgaggccc tggtcaacgc cgagatccgc gccaatcacg ccgtcgagac ccgcatcatg
     1621 ggcctggatg ccgcgaaggc ggccggcgcg atggcgctgt tcggcgagaa gtacaccgac
     1681 gaggtgcgcg tgctgtcgat gggcggcttc tcgaccgaac tctgcggcgg cacgcacgcg
     1741 agccgtaccg gtgacatcgg cctgttcaag atcgtcgccg aaggcggcgt cgccgccggc
     1801 gtgcgccgca tcgaggcggt gaccggcgcc aatgcctggg tgtggctgcg cgagctcgat
     1861 gcacgcgtca aggcgatcgc ggcactggtg cgcggcgatg ccggcagcgt cgagaccaag
     1921 gtgcgcgggc tggtcgatcg cgtgcgcggg ctcgaaaagg atgtcgagca gctgaaggcg
     1981 aagctcgcca gcgcccaggg cggggatctc gcggcgcagg cagtcgaggt cgccggcgtg
     2041 cgcgtgctgg ctgcgcgcct cgacggcgcc gatgccagaa cgctgcgcga gaccatcgac
     2101 cagctcaaga acaagctgca cacggccgcc gtcgtgctgg cgacggtcga ggatggcaag
     2161 gtgcggctgg tcgccggcgt caccgccgac cagaccgacc gcctcaaggc cggtgaactg
     2221 gtcaaccacg tcgcgctgca ggtcggtggc aagggcggcg gacgggccga cctcgcgcag
     2281 gccggcggca ccgatcctgc ggcgctcgat gccgcgctcg ccggcgtgcc ggcctgggtg
     2341 gccggacaga tcggctgatc cttattgtca tgcggccggt cgcggccggt cgcaccatta
     2401 cccgcaacac accggacacc tccagcatgg ctctcatcgt tcagaagtac ggcggcacct
     2461 cggtcggctc ggtcgaccgc atcgagaacg tcgcgaagaa ggtcatcggc tatcgcggcc
     2521 agggacacga tgtggtcgtc gtcgtctcgg cgatgagcgg cgagaccgat cgcctgatcg
     2581 ggctcgcgaa ggcgatcgat ccgcgcccga acccgcgtga gctcgacgtg ctgatctcga
     2641 ccggcgagca ggtgacgatc gcgctgctgt gcatggcgct cgaaaagcac ggccagccgg
     2701 cgcgttccta caccggcgcg caggtgcgca tcctgaccga cagcgcgttc acgaaggcgc
     2761 gcatcctcgc gatcgacggc gagaacatcc gctccgatct cgcgcagggc cgcgtcgtcg
     2821 tcgttgccgg cttccagggc gtcgatgagg acaacaacat cacgacgctc ggccgcggcg
     2881 gttcggacac caccggcgtg gcgctcgccg cggcgctgaa ggccgacgag tgtcagatct
     2941 tcaccgacgt cgatggcgtc tacacgaccg acccgcgcat cgagccgcgg gcacgccggc
     3001 tcgaccgcat caccttcgag gagatgctgg agatggcctc gctcggctcg aaggtcctgc
     3061 agatccgctc ggtcgaattc gccggcaagt acaacgtacc gctgcgtgtg ctctccacgt
     3121 tccaggaggg caacggcacg ctcatcaccc ttgaggacga gaatatggaa gctgcactga
     3181 tctccggcat cgccttcagc aaggacgaag cgcagatcac ggtgaccggc gttcccgatc
     3241 gcccgggcat cgccttcggc atcctcggcg ccgtctcgga tgccaacatc gaagtggaca
     3301 tgatcgtcca gaacatcggg cgtgacggca ccaccgactt cacgttcacc gtgcaccgca
     3361 acgacttcga gcgggcgctc gagatcgtgc agggcaaggc cgtggagctc ggggcccgcg
     3421 agatcaacgg cgaccgcaag atcgtcaagg tctcgctggt cggcgtcggc atgcgctcgc
     3481 acgccggcat cgccagccgg atgttccagg cgctggcgcg cgagggcatc aacatccgca
     3541 tgatctcgac ctcggagatc aagatttcgg tcgtcatcga cgagaagtat ctggaactcg
     3601 gcgtgcgcac gctgcacgag gccttccagc tcgatcaggc cccggccgcc tgagcgtcgt
     3661 gtcttcccgc cccggcacgg ccgccgcgcc cggcggataa ccatgcgccg ggctgtgaat
     3721 tacgatcaca gcccggtaca gggcgggtgt tcttggttac agtccggtcg tccccctttc
     3781 cccgggggaa agcagccaaa acgactggac aatcaggtga tacacaagga gtacatccat
     3841 gctcatcctg acccgcagag tgggcgagac cttgatgatc ggtgacgaag tcaccgtgac
     3901 cgtgctcggt gtgaggggca atcaggtccg catcggcgtc aacgccccga aagacatcgc
     3961 ggtgcaccgc gaggaaatct acgaacgcat caagcgcgaa caggagggcg gcgccgaccg
     4021 tggcgagcac gagtgatcgc cggatgcttt gacggcagcg gagaggaacg atagaatccg
     4081 cggccttcgt ggatgccctg ttgcagggca ggcgattccg gagagatggc cgagtggctg
     4141 aaggcgctcc cctgctaagg gagtataggg gcttaaacct ctatcgaggg ttcgaatccc
     4201 tctctctccg ccagtcttct ggcagcgcag tagatcagca gttgacgaag ccaagcgaag
     4261 actgcataat gcgcgcctcg caattcagag cgcccgtagc tcagttggat agagtacctg
     4321 gctacgaacc aggtggtcgg aggttcgaat ccttccgggc gcgccaaatc gaaacccgag
     4381 tcgacatcga ctcgggtttt tttttgatat ccgcaggaat gcggacccgg aatgcctcag
     4441 cggcaaacct ttgtcgcgat gcacggattc gggccgggtg acctgccgtc ggtccggtac
     4501 tctgtgcatt gcacaaggca tctggagaag tgtgatcgat gagcggaatg cgacatggcg
     4561 gcgcggcgct tgtgctgctg gcgttgctgg ccacgatgcc actgccggcg cccgcagcga
     4621 aatccgaagg tgcatcgacc tactacgaag acgccctgat ccgcttcgac cgcaatgatc
     4681 tggacggcgc ggaaatccag atcaagaacg cgctgcagca gaaccgcgac catctggcct
     4741 cgcacatcct gctcgggcgc atctacctgc tcaagggccg ggccgaggcc gcggtcttcg
     4801 aactgcagac ggccaatcgc ctgggtgccg atcgcaccac caccgatccg taccgggcac
     4861 gggcctatct gcagctcggt cgctacaagg atctcatcga caccgtcagc ggcgatggcc
     4921 tggcaccgcg cacgcgagcc gaggtgcttg ccgcccgcgg cagcgcgctg ctcgaactcg
     4981 gacagctgtt cgatgccgag caggccttcg ccagtgccaa ggatgccgac cccgcttcac
     5041 cggccggctg gtccggcatg acggtgctgc gcctgcgcca gggcgcgctg gtgcaggccg
     5101 agcaggaagc ccagcacgcc gtcgatctcg gcccgggcga tgccgaagcc tggaatgcgc
     5161 ttgcctcggt gatgcatcag tccggcaagg acgacgaagc tctggctgcc tacgcccggg
     5221 cactcgaggc cgatccgcgc atgctcgccg cgcgtctggc gcgggcgggg ctgctgctcg
     5281 atctcgatcg cctcgacgaa gccggtcagg acatcgagag cctgcgcaag gaatacccgg
     5341 tcgagccgcg cggcatgctg ctgcgggcgc tgtggttgca gcggcgcggt gaaaccgacg
     5401 cggcacgcgc ggcccttgcc gataccggtg ccgtgctgaa ggccgtgccg cgcgagacca
     5461 tggagcgctt tccggccctg ctgctggtct cgggcatgac gcattcggcg ctcggcgaac
     5521 tggagcgggc gcgtgacgat ctggcgcgct atctggagct ggcgcccgac aacccggggg
     5581 cgcgcaagca gctcggtgcc gtctacgtgc ggctcaatga accgacccag gcggtcgccg
     5641 tgctggagcc ggcgcggctg cgggtgccgg acgatccgcg catcctggcc ctgctcggca
     5701 tcgcgtactc gatgaagggc agccacctcg tcgcgaccga gctgctggaa aaagcctccg
     5761 ccctgagcgg tggcgctgcg gacatccgta ccgatctggc tgcctcgcaa ctgcggatcg
     5821 gacgtcagga gcaggcgctc gacgaactcg cgaccgtcta tgacaaggac ccggcccaga
     5881 cgcgtgccgg cgtgctgctg gccggcctgc acctgcagcg gggcgaggct gcgaaggccg
     5941 tcgacatcct gcagcagctg gtcgggcagg agccgcgcaa cctgaccttc ctgaacctgc
     6001 tcgcctcggc gctgctcgcc agtggcaggc agccggaagc gcggacgatg ctggagcggg
     6061 ccatcgagat cgatccggcc tttctgccgg cccagctcgg gctggcacgc ctggacatcc
     6121 tggcccgcaa gttcgatcag gcgcgcgcgc gcctgcaggc caggctgcag gccacgccgg
     6181 agaacgtgca gttgctggtc gagatggcgc gcgtcgatgc catcaccggc gcgcgcgaca
     6241 cagccatcca gcgtctggag cgcgcgcgca gcctgtcggc cgatgccatt gagccgcgcc
     6301 agttcctgat cgatctctac ctgcgtcgca acgaaggcaa tcaggcgctg gaagtggccg
     6361 aggagctcgt cgcgcggcat ccggacaatc tggcgtcgct cgccgccctc ggcaccaccc
     6421 agctcgtgct cggcaagggg ccgctcgcgc aggtgacgtt ccggcgcatg gtcaagtttg
     6481 ccggcttcga tccgatcgcc ctgatgcgca tcgcccgcct gcaggagcag gccggcgcct
     6541 tgaatgatag tgccttcacg ctcgaacagg ccgtgcatgc cgagcccaac cggcccgaac
     6601 tgctggccgc gctggccggt gcctatctgg tgaccggccg gctggaggaa gccgaggaac
     6661 aggcgcgccg gctgcagcag ctcgatcccg ccagtccggt gtcgcagcgt ctgctggccg
     6721 aggccctgtt gcgtcgcaac aagccggccg aagcggcggc gatgttccgc caggccatgg
     6781 cgaagacccc atcggcggaa ctggtgatcg gggctttccg ggccctgctc gccgatgacc
     6841 gggcggctga cggcatccgc atgctggagg actgggtcgc tcagcatccg gacgatcgca
     6901 acgtgcgtac cgcgctggcc gaaggctaca tgcgcgccga acgctggaag gaggcgctgc
     6961 cgctctacga agccctgctg gccgtccagc ccgaacaacc gcaggtgctc aacaacctcg
     7021 cctacatcgc gctgcagacc ggcgatgaag ccaaagccct cgactacgcc aggcgcgccg
     7081 ctgcgctggc gccccaggac ccggccatca ccgatacgct cggctggatt ctggtgcgcg
     7141 ccggacgtgc gcaggaagcc ctgcagtatc tgcgcgaagc gcagtcacgc gatgccggaa
     7201 atcccgaaat ccgttatcac ctcggtgtcg cgctgatgca gctcggtcgc aaggccgagg
     7261 ctcggcggca attcgagcag gcgatcgccg tcaagctggc ctatcccggc atcgaggaag
     7321 cccggcgtct gtatcgggaa accgctggtc cgtgaggagc ggctcgcaag acggggcctg
     7381 tccgtttgca gcaggccttg cgctttgcga ggctggggga aaatccggca gcattcacag
     7441 tgcaagtatt tgattgattt gctgattaat gaatggctcg atttttgctg atccgggatt
     7501 ggtgtgcagg atcgaaatgg tttcctgcac ggtgattcca atcgggggcg agccatggat
     7561 atcagacgag ccgcgttgtt tgcgggtatg gcttcgatgc tgctggccgt aacggccggg
     7621 gccggagttt cgtatacgtt tgcgaccgac tgcgcggcag ggaccggtgc gtcgctgagt
     7681 tgcgccaatg ggcggctcgg ggtttcggca tggtcgaata ccgtcggtac ggccaatcgt
     7741 tacatcgaga atgcgaccgt cgtcaattac ggcagttacg gactcggcat caccaatgcc
     7801 gacagcagcc tgccgagcgg tgccgatgcc ggtgaaggga cttcgcccga gcacgccatc
     7861 gacaacaatg gtcgctatga cgcgctgctg ctggatttcg gagccggcaa ccagttcggg
     7921 ctgacggggt ttcgcaacgg ctggtacagc accgattccg acatgagctt cctcgcctat
     7981 accggcagcg gggatgcgct ggccggtctg gcgagtcgta cgctcagttt cggcgacgcg
     8041 gccagcgatc cggcgagcct gctgaacaat ggctggaccc gggtcagtga ctacgcggat
     8101 gccggtacgg tggcgcgatc cctgaccgcc ggcgtggcgt cacgctactg gctggtgtcg
     8161 gcctacgtgc cgatgtcggc gacctacagc ctttcggtcg ggaacgatta cgtgaagctg
     8221 gcggccatcg agggttcgta cctgcgtcag gtgccgctgc cggcgacctg gtggctgctg
     8281 gtgcccggct tgctctggct ttaccgcttg cgcagccgca gggcctgatg caatggcggt
     8341 gccggcggcg gtcgaggctg ccggcttgcg gtcggaaaaa tcgacgcggg aaatcaggcg
     8401 ggaagattcc ggcagaaaat gccatgaccc taaaacgaaa cagccggcgc atgggccggc
     8461 tgtttcgttt ctggaatttg gtcggggtga caggattcga acctacgacc cccacgtccc
     8521 gaacgtggtg ctctaccagg ctgagctaca ccccgaatga tcgctgacga gtgttaatgg
     8581 gagcgggaaa ccgagtaatc ggccagtgca atcagagcct gcttttgaat ggattccggg
     8641 agaatccgca gggcttcgat tgctgcatcc gcttcctgtc tagcgaggcg cgcagtatag
     8701 gcaaccgccc cggtcgatgc aatagcttcg gtaatgacgc cgatctgatt gcgatcgccg
     8761 ttttcgatcg cctgacggat catggacgcc tgcgcggccg agccgtgacg catcgcatag
     8821 atcagcggca gcgtcggctt gccttcggcg aggtcatcgc cgacgttctt gccgatctcg
     8881 tcgctggagg cgctgtagtc gagcaggtca tcgaccagct ggaaggcggt gccgaggcgc
     8941 atgccgtact cggcaagcgc ctgttcgatg ccggccggat ggccggcgac gaccgcgccg
     9001 agccggccgg cggcctcgaa cagcttcgcg gtcttgctgt agatgacctc gaagtagcgg
     9061 gcctcgtcga catcggggtc gtggcagttc agcaactgca ggacctcacc ttcggcgatg
     9121 cggttggtgg tgtcggcaag gatgcgcatg acgcgcatgc tgtcgagccg caccatcatc
     9181 tggaaggccc gcgagtacag gaagtcgccg accagcacgc tggcctcgtt gccccagatg
     9241 gcgttggcgg tttcccgccc gcggcgcagt tccgaggcat cgaccacgtc atcgtgcagc
     9301 agcgtcgcgg tatggatgaa ttcgacgatc gccgccagat cgatgtgatc gttgccggca
     9361 tagccgcagg cacgggccgc cagcaccgtc agcaccgggc gcatgcgctt gccgccgctg
     9421 ttgatgatgt aggcacccag ctgattgacc agcgccacgt ccgagtgcag ttcgcgctgg
     9481 atgagccggt cgaccgcctg catgtcggcc tcggcgaggg cgcggacttc agagatgttc
     9541 atgatgttcg atcgggcttc gagggtccga ggtggcgggc tggatttgag aggcggggcg
     9601 ctgggcgtgg ccgcgagccc gcaagtctac cgccggtgcc ggccggggga cagcatcgaa
     9661 tccgattgac cggatggcgg gcacatgact agaatcgcgc ccctttgatg gtcactcatc
     9721 gaattctgtt tggagacttc aggaatgtac gctgtcatca agaccggcgg caaacagtac
     9781 cgtgttgccg agggtcagac cctcaaggtc gagaagctcg acgtcgaaga aggtgcctcc
     9841 gtcgagctgg cggaagtcct gctggtttct gacggcgaga cggtgaccgt cggcgcgccc
     9901 acggtcggcg gcgccatcgt cacggctacc gtgcagtcgc atggtcgggc caagaaggtc
     9961 gaaatcgtca agttccgccg ccgcaagcac catcgcaagc agatgggcca ccggcagcac
    10021 tacaccgaac tgaagatcac cggcatccgt gtcggctgat cgggagctct gatcccatgg
    10081 cacacaagaa agccggcggc tccagccgca acggacgcga ttccgaatcc aagcgcctcg
    10141 gcgtcaagat gtacggtggt cagctctgca tcgccggcaa catcctcgtc cgccagcgcg
    10201 gcacgcagtt ccatcccggc gtcaacgtcg gtctgggcgt cgaccacacg ctgttcgcga
    10261 aggccgatgg tcgtgtcgtg ttcgagacca agggtccgga ttcgcgcaag tacgtgagcg
    10321 tcgtgccggc ctgaaccccc ggtttcgcgc tgtcgagagc cccgtcgcgt gcggggcttt
    10381 ttcatttccg ccgccgctgc gggcgcggcg tcaggcaggt gcagcatgaa gttcgtcgac
    10441 gaggcggcca tccgggtcat ggccggggac ggcggcaacg gatgcgtgag cttccgccgc
    10501 gagcgcttca tcccgttcgg cgggccggat ggcggggatg gcggcgatgg cggcagcgtc
    10561 tggctggtcg ccgatgcgga cctgaacacg ctcgtggact tccgcttcac gcgcacgttc
    10621 aaggccggcc ggggcgagaa cggcggcgga gccaaccgca ccggcgccgc cggggcggat
    10681 ctcgacgtgc cggtgccggt cggcacgact gcgatcgatg ccgacaccgg cgagacgatc
    10741 ggtgacctga ccgcgccggg ccagcgcctg ctggtcgcgc agggcggctt ccacggcatc
    10801 ggcaacgccc gctacaagag ctcgaccaac cgcgcgccgc gccagtccaa gcccggcacc
    10861 accggcgagg tgcgcgcact gcagctcgaa ctgaaggtgc ttgccgacgt cggtctgctc
    10921 ggcatgccga acgccggcaa gtcgacgctg atccgtgccg tgtcctcggc gcggccgaag
    10981 gtggccgatt acccgttcac gacgctgcat ccgaacctcg gcgtcgtgcg cgtcgggccg
    11041 ttgaagagct tcgtgatggc cgacattccc ggcctgatcg aaggtgcggc cgaaggggcg
    11101 gggctcggtg tgcagttcct gaaacacctg gcgcgcacca gcctgctgct gcacctcgtc
    11161 gatctcagtc cgtacagcga cagcggcgac ccggtcgccg atgtgcgcac gatcgaggcg
    11221 gagcttgcca agtacagcgc cgaactcgcc ggacgcgagc gctggctggt gctgaacaag
    11281 ctcgacctgc tgcccgaaga cgaacgcgag gcgcgcgcgc aggcggtcgt cgaggcgctc
    11341 ggctggtcgg ggccggtgtt ccggatcgcg gcgatcgggg ccgaaggcac cgagccgctg
    11401 tgctggcgga tcatggagcg gctcgatgcc gcgcgcgccg cgcggccggc cgatgctgcg
    11461 ctgccgatcg attccgaagg cgagggcgcg tgatggccgt ctcgcgcgaa acgctcggac
    11521 aggcgcgccg ctgggtcgtg aagatcggca gcgcgatgat caccgacgag gggcgcggtc
    11581 tcgatgccgc ggcgatcgaa gcctgggtcg cgcagctcgc cgagctgcac gccgagggcc
    11641 gggacgtgat catcgtttcc tccggcgcgg tcgccgaagg catgcgccgg ctcggctggc
    11701 gcgcacggcc gaaggcgctc gccgagctgc aggcggcggc agccgtcggt cagatggggc
    11761 tggtgcaggg ctgggaggcg gcgttccaga agcacggcat ccgcaccgcg cagatactgc
    11821 tgacgcacga ggatgtctcc gaccgccagc gctacctgaa cgcccgcaat acgctgcgca
    11881 cgctgacgcg gctgcgcgtg gtgccggtca tcaacgagaa cgacacggtc gcgttcgagg
    11941 agatccgctt cggcgacaac gacacgctcg gcgcgctggt cgcgaatctg gtcgaggccg
    12001 agctgtacgt gatcctgacc gaccagcacg gcatgtacga ccgcgatccg cgcgcgcatg
    12061 ccgatgcccg cttcctgtcc gaggtcagcg ccggcgatcc ggcgctcgaa cggatggccg
    12121 ggggcggcgc cggtacgctc gggcgtggcg gcatgctgac caaggtgcgc gcggcggcga
    12181 aagccgcgcg ctcgggcacg gcgacgctgc tcgcctgggg gcgtgagccc gaggtgctgc
    12241 ggcgcgtcgc gcgcggcgag ccgatcggca cgctgctgcg gccgcaggcc gggccggtgg
    12301 cggcgcgcaa gcagtggctg gccgtgcagt tgcaggtgcg cggctggctg cacctcgatg
    12361 ccggcgccgt gcaggcgctg aagctcggcg gacgttcgct gctcgcggtc ggcgtgcgcg
    12421 gcgtcgaggg cgagttccag cgcggcgagc tggttgcctg cgtcggcccg gatggcgtcg
    12481 aggtcgcgcg cggcctggtc aattacgacg cgtcggaatc ggtgcgcatc atgggcaagc
    12541 cgaccgaggc gatcgaatcg gtgctcggct acgtcgatgc gccggcactg atccatcgcg
    12601 acgatctggt gctggtctga cgcagctggt ctgatgccgg cactgcccgc tgccgcaggg
    12661 cggcgggcga tccggtccgc cggaatcgag gcatgaaaat cgaggcatga aaaaagccgg
    12721 gtcgccccgg ctttttcggt gtggcggcag gcgtgtgctt acgccatcgc cttgatgtgc
    12781 gcggacaggc ggctcttgtg gcgggcggcc ttgttcacgt ggatcaggcc cttgcgggcg
    12841 gtgctgtcga tcacggaggt cgccgagcgg aacgcggctt cggcctgcga cttgtcaccg
    12901 gcacggatcg cggcgatgac cttcttgacg taggtacgga agcgcgaacg caggctggcg
    12961 ttgtgctggc gggccttctc cgcctggcgg acgcgcttct tggcttgggc agtattggcc
    13021 actgtcgggg ctcctggaat tcgtgtgatg cagtcgatgc agtgctgcga ggctgcggac
    13081 gcccgggcag tggcggccga cgctggaaaa ggcgcgaatt gtccgcgtcc ggcgggccgc
    13141 tgtcaatgct gcaggcgcgc tggcgagggc tgcgcacgct gcttatgatc cgcgctcccg
    13201 cgggccgggg cccggaaaaa gaaatcctga aaagaaatcc ttacatgagc agcaagtccc
    13261 tgatgcggtc caccggcgtg gtcagcgcgc tgaccttcct gtcgcgcctg ctcggcttcg
    13321 tgcgcgacat ggtctacgcc ggcctgttcg gcgccggtgc cggcatggat gccttcaacg
    13381 tcgccttccg cattccgaac ctgttccggc ggctggtcgg cgagggcgcc ttctcgcagg
    13441 ccttcgtgcc ggtgttctcg cagtaccgca cgcagcgcag ccccgaggag ctgcgcctgc
    13501 tggtcgatca ggtggccggc acgctcggga ccgtgctgct cgcgctgacc gccttcggca
    13561 tgatcggcgc gccgctgctg gtctgggcgt tcgcgagcgg ctacggcgat gagccgtaca
    13621 agttcgcgct gaccgagcag atgctgcgca tcacgttccc gtacgtgctc tgcatctgcc
    13681 tgaccgcgtt cgccggcggc atcctcaaca gctgcgggca cttcgcggtg ccggcggtga
    13741 cgccggtgct gctgaacgtg acgatgatcg ccgcggcgct gctggcggcg ccgcacttcg
    13801 ccgagccggc gctggcgctc gcctggggcg tggcgcttgc cggcgtgctg cagctgctgt
    13861 tccagtggcc ggcgctgaag cgcatcgggc tggtgccgcg cccgcggctg gcctggcgtg
    13921 ccgagggcgt gcgccggatc atgatgctga tggtgccggc gctgatcggg tcgagtgccg
    13981 cgcagatcaa cctgctgatc aacacccagc tcgcctcgac gctgccgacc ggcagcgtgt
    14041 cgtggctgta ctactcggac cggctggtcg agttcacgct cggcatcttc ggtgcggcgc
    14101 tcggcaccgt gatcctgccg aagctgtcgc gccagcacgc cgcggccgaa ggcgagggct
    14161 tcgcgcacac cctcgactgg gggctgcgct ggtcgctgct gatcggcacc cccgcgaccg
    14221 tcgcgctcgc gatcctcgcc ggtccgctgc tcgccacctt gttcatgcgc ggcgagttca
    14281 cggcgaccga tgtcgagatg accacgcgca gcctgatgac ctacggcagc ggcctggtcg
    14341 cctacatgct ggtcaaggtg ctggcgcccg gcttctacgc gcgtcaggac acgaagaccc
    14401 cggtgcgttt cggcctgatc tcgatcggcg ccaacatcgg cttccagatg ctgctgatcg
    14461 cgccgctcgc gcacgcaggc cttgcgctgt cgacggcact cgccgccttc gtcaacgtcg
    14521 cgctgctgta ctggcagctg cgccgcgacg gcgtctaccg gccgcggccc ggctggccgg
    14581 cgttctggct gcgcatgctc gcggccaacg cggcgatggc gggctttctg gtctgggcgg
    14641 ccggcgatct ctcgggctgg atcgccgccg acacgctgac gcgcgtgctc cggctcgcgt
    14701 ggatcgtgcc ggccggggcc ggcgtctacg cggcggtggc gctcgggctc ggcgtgcggc
    14761 tgtcgcagct gcgcgcgccg gcctgatggc gcgggccggc atgacaagcc cgggcgcttc
    14821 cttataatcg ctcgcttgct ttttcagcgg cgcgacgcgc ccggagcagc ccggcacccg
    14881 gcatggaact gatacgcggc acgcacaatc tgcgcccccg acaccgcggc tgcgtcgcga
    14941 ccatcggcaa tttcgacggc gtacacctcg gccaccgcgc ggtgcttgac cagctgcacg
    15001 cgcgtgccga tgcgctcggc ctgccggcgg tggtgatcac cttcgagccc tacccgcagg
    15061 aatacttcgc gcgcagccag ccgccggcgc ggctgacgcg gctgcgcgag aagctggtcg
    15121 cgctcgaagc cgccggcgtg cagcgcgtgc tggtgctggc cttcaatgcc cggctcgcgg
    15181 cgatgcgcgc cggcgatttc gtgcagcacc tgctggtcga cggtctcggc gtgcgcttcc
    15241 tcgtggtcgg cgatgacttc cgcttcggcc acggccgcga gggcgactac gcgctgctcg
    15301 aacgctgcgg tgcggcggcc ggtttcgagg tcgcgcacac cgccacgcac ctgatcgatg
    15361 gcgtgcgcgt cagcagcacg cgcatccgcg agcggctggc ggccggtgag ctcgccgagg
    15421 ccgcgcgcct gctcgggcgg ccgtacgcgg tgcacgggcg cgtcgcgcat ggccagcagc
    15481 tcggccgcac catcggtttt ccgaccgcca acgtacacct gcgccggcgc gcgacgccgc
    15541 tcggcggggt ctacgccgtg gtgctgcgca gccccggcct gccgccgctc gccggggtcg
    15601 ccaacctcgg ccgccggccg acggtcggcg gcacgcgcga gcagctcgaa gtccactgct
    15661 tcgatttcga cggcgacctg taccggcggc gcgtgcaggt cgagttccgc gagcggctgc
    15721 gccccgaaca gcgctttccg tcgctggacg aactgaagct ccagattgcc cgggacgccg
    15781 aggccgcgcg tgcgcatttc cgcgcccggc cggcggactg aactccgact gcatggcgcc
    15841 gcaagcggcg cgaggtgagc atggccgatt acaaggacac cctgaacctg cccgacacgg
    15901 ccttcccgat gaagggcaac ctgccgcagc gcgagcccga gcagcttgcg cgctgggcag
    15961 ggatgaacct ctacgcgcag cagcgcctcg cctgcgccgg ccggccgaag ttcgtgctgc
    16021 acgacggtcc gccgtatgcg aacggcgaca tccacatcgg ccacgccgtc aacaaggtgc
    16081 tgaaggacat catcgtcaag tcgcgcacgc tcgccggctt cgatgccccc tacgtgccgg
    16141 gctgggactg ccacggcctg ccgatcgagc acgccgtcga gaagaaggtc ggcaaggtcg
    16201 gcgtcaaggt cgatgcccgc agcttccgcc agcagtgccg cgccttcgcg cacggccagg
    16261 tcgatcgcca gcgcgaggac ttcatccgcc tcggcgtgct cggcgactgg cagcacccgt
    16321 atctgaccat ggacttccgc accgaggccg acatcgtgcg ttcgctcggg cgcatcgtcg
    16381 gcaacggcca cctgctgcgc ggctcgaagc cggtgtactg gtgcctcgac tgcggctcgg
    16441 cgctcgccga ggccgaggtc gagtacgagg acaaggattc gctggccatc gacgtgcgct
    16501 tcccggtggt cgaggccgcg agcgtgttcg cgcgcctgca ccacgccgag ggcggtgacg
    16561 gcaaggggcc gctgtcggtc gtgatctgga cgacgacgcc gtggacgctg ccggccaacc
    16621 gcgccgtgac gctcggggcc gaactcgaat acgtggtggt gcaggccgag accgcgcagg
    16681 gcagcgaacg cctgctgctc gccgagggcc tgctcaagga ctgcatgctg cgctggggcg
    16741 tcgagcgcta tcaggtgctc gcctacggca agggcgcgga cctcgaaggg ctgaagctcg
    16801 cgcacccgtt ctatgagcgc gaggtgccgg tgatcctcgg cgagcacgtc acgctcgaag
    16861 ccggtaccgg ctgcgtgcac acggcgccgg cgcacggcca ggacgactac gtcgtcagca
    16921 gccgctacgg cctgcgcatc gacgacaacc cggtcggccc ggacggccgc ttcctgcccg
    16981 aaaccccgct gttcggcggc ctgcacgtgc acggcgccaa cgagcgcatc ctcgacgtgc
    17041 tcaagggcca cgcccggctc gtgcatgccg aaaagctgcg ccacagctac ccgcactgct
    17101 ggcggcacaa gacgccgatc atcttccgcg ccacgccgca gtggttcatc tcgatggagc
    17161 aggccggcct gcgcgcgcag gcgctcgccg ccgtcgaaca gctgcgcttc acgcccgact
    17221 ggggccgggc gcgcctcgaa ggcatgatcg ccaaccgccc ggactggtgc atctcgcgcc
    17281 agcgctactg gggcgtgccg atcgtgctgt tcacgcaccg gcgcagcggc gagctgcatc
    17341 cgcgctgggc cgaactgatc gagcaggtgg cactgaagat cgagcaggag ggcatcgacg
    17401 gctggttttc gagcacggcc gaggactggc tcggtcccga ggccgccgac tacgagaagg
    17461 tcaccgacac gctcgatgtc tggtacgact ccggcacgac gcacgcctcg gtgctggagc
    17521 gccgccccga actcggcttc ccggccgatc tgtacctcga aggctcggac cagcaccgcg
    17581 gctggttcca gtcctcgctg ctcgcctcgg tggcgatgcg cggcgtggcg ccgtacaagg
    17641 gcctgctgac gcacggcttc acggtcgacc agcagggccg caagatgtcg aagtcgctcg
    17701 gcaacgtcat cgccccgcag gaggtcatca aggaactcgg cgccgacgtg ctcaggctct
    17761 gggtcgccgc caccgactac cgcggcgaga tgacggtctc gaaggaaatc ctgcggcgca
    17821 tggccgattc gtaccggcgc atccgcaaca ccgcgcgctt cctgctcgcc aacctcaacg
    17881 gcttcgatcc ggcgcgcgat gccgtcgagc cggcgcgcat gctgccgctc gatcgctggg
    17941 tcgtcgaccg cgccgcccgg ctgcaggccg aggtctgctc ggcctatgac gactacctgt
    18001 tccacgtcat ctaccagaag gtccacaact tctgctcggt ggacctcggc agcttctacc
    18061 tcgacgtgat caaggaccgt cagtacaccg gcgcccgcga cggcctgccg cggcgctcgg
    18121 cgcagaccgc gctgtatcac gtgctcgaag ccctggtgcg ctggatcgcg ccgatctgtt
    18181 cgttcacggc cgaggaaatc tggaagcaca tgcccggtgc gcgcgccgag agcattttcc
    18241 tcgacaccac ctggtacccg ggcctgttca cgctcgatgc cgacgatgcc ttcggtgccg
    18301 actactgggc gcggctgatc gacgtgcgcg gcgcggtcag caaggcgatc gaggacctgc
    18361 gcaagggcgg cggcatcggc ggctcgctgg aggccgaggt cgacctgtac gccgacgaaa
    18421 gcctgcgcac cgatctcgcc cgcctgcacg acgaactgcg cttcgtgctg atcacctcgc
    18481 aggcgcgcgt gcatccgctc gatcaggcac ccggctccgc cgtgctcgcc ccggacggcc
    18541 ggctggcgct ggtcgtcgcg cgcagcgagg cgcagaagtg cgtgcgctgc tggcacctgc
    18601 gccccgatgt cggcacgcat gccgcgcacc cgcagctctg cgggcgctgc atcgagaacg
    18661 tcgatcaggc tgctgcaggc ggcgaacgcc ggctgtacgc ctgatggcgc gctggctttg
    18721 gctgagcgtg gccgtgctcg cgctcgatca gatcagcaag tacgccgccg agaccctgct
    18781 gctgctgcac cggccggtgc cggtgctgcc gccggtgctc gacttcatgc tcgcctacaa
    18841 caccggcgcg gcgttcagct tcctcgccgg agccggcggc tggcagcgct ggttcttcat
    18901 cgtgctggcc ggcgcggtat cggtggcgct gacggtctgg atcgtgcgcc tgcatgcgca
    18961 tgagcggcgg ctggccgtgg cgctggcgct ggtgctcggc ggtgcgatcg gcaacctcgt
    19021 cgaccgcgcg ctgtggggcc acgtcatcga cttcatcctc gtgcatttcg gcgactggcc
    19081 gttcccggcc ttcaacctcg ccgattcggc gatcaccgtc ggcgcggccc tgctcgtgct
    19141 cgacagcctg ttcccgcacc gggcgcccga cgcctgagga gtccccgcca tgcagatccg
    19201 cctcgccaat ccgcgtggtt tctgtgccgg cgtcgaccgc gccatcgaga tcgtcgagcg
    19261 cgcgctcgac ctgttcggcg cgccgatcta cgtgcgtcac gagatcgtcc acaaccgcta
    19321 catctgcgac aagctgcggg ccgaaggcgc gatcttcatc gaggatctgg ccgaggttcc
    19381 ggacggctcg acgctgatct tctcggcgca cggcgtctcg cgcgcgatcc acgacgaggc
    19441 cgcccgccgc ggcctcaagg tgttcgacgc cacctgcccg ctggtcacca aggtgcacat
    19501 ggaggtcgtg cgctatgcca agcagggcat cggcgtcgtg ctgatcggcc acgccggtca
    19561 ccccgaggtc gagggcacga tgggccatta cgaccggtcg gccggcggcg ccatccatct
    19621 ggtcgagacc gtcgccgacg tggcgcgcct cacggttgcc gatccgcagc gcctggcctt
    19681 cgtcacgcag accacgctgt cggtcgacga cacggtcggc atcatcgatg cgctgcgcgc
    19741 gcgcttcccg gccatccacg gcccgcgcaa ggacgacatc tgctacgcga cccagaaccg
    19801 tcaggacgcg gtcaaggaac tcgccgccga ctgcgacgtg gtgctggtgg tcggttcgcc
    19861 gaacagctcg aactccaacc gcctgcgcga gatcgcgcag aagctcgacg tgccggccta
    19921 tctgatcgat gccgccgagg acatcgatcc ggactggctc gccggcaggc agcgcgtcgg
    19981 cgtcacggcc ggcgcctcgg cgcccgacgt gctggtgcag cgcgtgctgc agcgcctgcg
    20041 cgacgaatgc ggcgccgagg tgcagcccga cgatggcggc atcaaggaga acgtcgtgtt
    20101 cgcgctgccg cggga
//
LOCUS       DAOQIE010000024        19342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1279_length_19342_cov_7.824234, whole genome shotgun sequence.
ACCESSION   DAOQIE010000024 DAOQIE010000000
VERSION     DAOQIE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 19342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19342
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_19342_cov_7.824234"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /gene="gluQRS"
                     /locus_tag="PK144_02885"
     CDS             complement(<1..643)
                     /gene="gluQRS"
                     /locus_tag="PK144_02885"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNF64700.1"
                     /translation="MSPYTGRFAPSPSGPLHFGSLVTALASWLDARAAGGRWLLRIED
                     VDGPRTVAGAEALILADLERHGLHWDGEVLRQSRRTAVYEAALAQLQAAGRLYACTCS
                     RARLAASGAASGPAGLVYPGFCRNAGHAQAPGRALRLRIDDAMTVGFTDRLQGPQAQC
                     LGREVGDHVLRRADGLIAYALAVVVDDGEQGVTHVVRGADLLTSTPRQIFLQQQ"
     gene            773..1252
                     /locus_tag="PK144_02890"
     CDS             773..1252
                     /locus_tag="PK144_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF64701.1"
                     /translation="MTIQSLPLLHVIEPIPASDSALAALNLMLDLAINHLPVVDAHGH
                     YLGMLTLTDLLHEILPAGLRLGHGIGQPDVSFLGDAASLLSTHLGQLSARTAGDVADP
                     HVVPLETDCPLVEAARRLAEARNPLPVVDADRHIVGVLSVRLLLTHLLGRGVQNDAR"
     gene            1242..2582
                     /locus_tag="PK144_02895"
     CDS             1242..2582
                     /locus_tag="PK144_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNF64702.1"
                     /translation="MHGEPASVSHVIFGLPPLLVSLAIFVATYAIVISEKLNRAVIAA
                     LGAGLMILSGVLTQLEAVHGIDFNTIGLLTAMMVIVAITQKTGVFQYVAIVAAKRVKA
                     DPWGLLVMMAVVTAVFSAFLDNVTTVLLVTPVILLITEALGVPPYPYLFTVIFASNIG
                     GTATLIGDPPNIMIGSAANLSFNTFLLHLTPVVLVVFAATLLPIWLVWGRTLRATPEA
                     RAQVMAFDEKSAITDPRLLKQALAVIGAVIFGFMIAHPLHLEPATIAGFGAALLLALQ
                     VVRHDAEHQTEEVAHALREIEWVTIFFFVGLFVVVTGIEKAGALKLMADWIVAATQGD
                     YKATAMAILWVSAVLSAVVDNIPFVATMIPVIQNMAPTFGADNLLPLWWALSLGACLG
                     GNGTLVGASANLVVAGFAERAGQPIRFKTFLLMAFPLMLMSIAISTVYLYLRYL"
     gene            complement(2652..3914)
                     /locus_tag="PK144_02900"
     CDS             complement(2652..3914)
                     /locus_tag="PK144_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF64703.1"
                     /translation="MAIHEFFLMLAVLLLTARLFGELASRLGAPSVIGELLAGLVLGP
                     SVLGWVQPTETLMTLAEVGIILLLFEVGLDTNLFRLAEAGRKPFVVAAVGAFLPLVLG
                     FAAGWLLFDTSILAALFIGGTLTATSIGITVRVLDDLMRRRSDEAQIVIGAAVIDDIL
                     GVIILAFLYDFSSPKGVSLASTASIALFIVVFMVLAPIAGKIIAWAIDHTDRKLESEG
                     LLITLVISLIMLFSWLAHAVGAPAIMGGFAAGIALSQHFRMSITQRLRLPFRRTLERA
                     FAPRPELTHRLEKQFRPLLHTYTPIFFVMVGVSLDLSAVDWSSARVWLMAGVLVVLAV
                     AGKVASGWFIAEPTLRRAAIGLSMVPRGEVGLIFAKLGLGAAILSQELYAVLLIVVAL
                     STLLPPFALKAFYQRYGDRPELVMRDDD"
     gene            complement(4148..6523)
                     /gene="ppsA"
                     /locus_tag="PK144_02905"
     CDS             complement(4148..6523)
                     /gene="ppsA"
                     /locus_tag="PK144_02905"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNF64704.1"
                     /translation="MAEYVVWFEALGMGDVDRVGGKNASLGEMISHLSAAGVQVPGGF
                     ATTAEAFREFLTVNGIIDRINAELAKLDIEDVNALAETGARVRQWVIDAPFQPALLDA
                     IETAYRDMTSRYGAELSVAVRSSATAEDLPDASFAGQQETFLNVRGLDQVMAAIKHVF
                     ASLYNDRAIAYRVHHGFEHGEVALSAGIQKMCRSDLGAAGVMFTLDTESGFDDAVFIT
                     ASYGLGETVVQGAVNPDEFYVHKPTLAAGRPAVLRRNLGGKAIKMVYNSAEQTVGRSV
                     KTVDVELAERRRFALADAEVEALARQALIIERHYGRPMDIEWGKDGTDGQLYILQARP
                     ETVKSRADSQVMQRYQLSRRGEVIATGRSIGQRIGSGVVRVISDIKEMAKVQAGDVLV
                     TDMTDPDWEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERVRDGEPVT
                     VSCAEGDTGYIYRGLIDFDIREVSLDAMPQLAFKIMMNVGNPDRAFDFSRLPNAGVGL
                     ARLEFIINRMIGVHPKALLNYDALPSDIKVRVDSQIAGYASPVDFYVDKLAEGIATLA
                     AAFAPKPVIVRMSDFKSNEYANLIGGPLYEPHEENPMIGYRGASRYVSESFRDCFELE
                     CRAMKKVRNDMGLDNVQVMIPFVRTLEEARQVVEILAANGLERGRDGLKLIMMCELPS
                     NALLADEFLEYFDGFSIGSNDLTQLSLGLDRDSGLIAHLFDERNPAVKKLLSMAIQAC
                     RRNGKYIGICGQGPSDHPDLAKWLMEEGIESVSLNPDTVVETWMFLAGEAA"
     gene            6769..7245
                     /locus_tag="PK144_02910"
     CDS             6769..7245
                     /locus_tag="PK144_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HNF64705.1"
                     /translation="MIFPVRAHAGLDPRPGSFSGLMDLYERNYMLLRRLLPAMLPADA
                     RQVSQAAGALDLHLHVSERFRYTSELSLTYRFEHGDGQVLLEPDLHVRIYHDARLAEV
                     MVARLRHAPPYRLGAAEEASELYARCRANRFLYKWLCYCTRQGHGFASATGAPHNS"
     gene            7335..7847
                     /locus_tag="PK144_02915"
     CDS             7335..7847
                     /locus_tag="PK144_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HNF64706.1"
                     /translation="MKLSTKGRYAVTAMMDLAIHDSHGPVTLADISQCQGISLSYLEQ
                     LFARLRRSGLVEGVRGPGGGYRLARRADAISVADIIAAVDERVDVTRCQGRGDCDNGR
                     QCLTHDLWNDLSERIATFLTGITLADFVDRPAVREMAERNALRQTQGHGHGQTQARRQ
                     LTVRERRISA"
     gene            complement(8131..8565)
                     /locus_tag="PK144_02920"
     CDS             complement(8131..8565)
                     /locus_tag="PK144_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNF64707.1"
                     /translation="MSDLHGYYIEDLSVGQSASYAKTVTEADVILFAGITGDDNPVHI
                     NQEFAETTMFKGRIVHGIFCGGLISTVAGTRLPGPGAIYIEQSLKFKAPVRIGDTVTA
                     TVTVTEINAAKRRVHADTVCTVHGKVVVEGKATFMVDNRPAG"
     gene            8810..9559
                     /locus_tag="PK144_02925"
     CDS             8810..9559
                     /locus_tag="PK144_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNF64708.1"
                     /translation="MKVLVAVKRVVDYNVKVRPKADGTGVETANVKMSMNPFDEIAVE
                     EAVRLQEAGKASEVVVVSLGVSACQDTLRTALAMGADRAILVETDAELQPLAVAKLLK
                     AVVDKEQPGLVILGKQAIDDDCNQTGQMLAALLGCGQGTFASKVEPGDGLVTVTREVD
                     GGAETVELKLPAVITSDLRLNEPRYVKLPNIMKAKKKPLEVLKPEAFGVDVTPRLKTL
                     KVAEPPKRAGGVKVASVAELVDKLKNVAQVL"
     gene            9562..10488
                     /locus_tag="PK144_02930"
     CDS             9562..10488
                     /locus_tag="PK144_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF64709.1"
                     /translation="MAVLVIAEHDNAVLKAATLNTVTAAAGLGEVHVLVAGQGCGAVA
                     EAAAQVAGVSKVLLADDAALAHALAENLAPLVTGLAGGYSHVLAPATAAGKNVAPRIA
                     ALLDVAPVSDIIEIVSPDTFVRPIYAGNVLATVQSADPVKVVTVRGTAFAAAAASGGS
                     APIERISGAADSGVARFVGQEIMKSERPELTSARVVVSGGRGLGSGENFAILEALADK
                     LGAAVGASRAAVDAGFVPNDYQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVA
                     INKDEEAPIFQVADYGLVGDLFQLVPELTQSL"
     gene            10596..12377
                     /locus_tag="PK144_02935"
     CDS             10596..12377
                     /locus_tag="PK144_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNF64710.1"
                     /translation="MSSYTAPVRDMRFVIGELVDLDAIAALPGCEDVGPDLVEAVLEE
                     AGKLAGNVLDPLNQQADRNGARWTEAGVVAAPGFAEAYQQFIEGGWNGLACPTEYDGQ
                     GLPELLGTATHEMWNAANMAFALCPMLTLGAIEAIQHYATDELKAAYLANMVAGTWTG
                     TMNLTEPQAGSDLAAVRSKAVPEGDHYRISGQKIFITWGDHDMTDNVVHLVLARLPDA
                     PAGVRGISLFLVPKFLLNADGTPGERNTVQCVSIEHKLGIHGSPTCVLAFEEAIGYLV
                     GTANKGLAHMFTMMNEARLKVGLQGLAQADRAYQQARWYANDRIQGRTVVDQGDARSP
                     IINHPDVRRMLMTMRAQAEAMRAVAYTTAAQVDIARRHPDEAVRKAAQARVDLMIPIV
                     KGWSTETGQDVTSIGIQVHGGMGYVEETGAAQYFRDARITTIYEGTTAIQANDFVGRK
                     LALDRGAAMQALLADMRATLAGLDAADGEDLAVIRTRLAPAVDALEEAADWVVANYEA
                     CQADVLGAAFNLLMMAGCVCGGWQMARAALAASAQLAAGGDPKFYGAKLVTARFYAEQ
                     LLPRAGALLTVAKAGAASMMALDPEQF"
     gene            complement(12493..13488)
                     /gene="dmeF"
                     /locus_tag="PK144_02940"
     CDS             complement(12493..13488)
                     /gene="dmeF"
                     /locus_tag="PK144_02940"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HNF64711.1"
                     /translation="MTEPAAPPCAHSHAFHSSAPQAEQRAWHVVWLTAVMMLVEILAG
                     WWSGSMALLADGWHMASHVVAIGLAALAYRHARRHAGDHRYAFGTWKVEVLGGFASAV
                     LLAAVAVLMAFESLLRLANPVDIAFGEAMGVAAIGLLVNLASAWLLHDDGHEHGTHAH
                     EAAHAHGHGHGHGHDAAGCHEAGQPHAHGHDLNHRAAYLHVLADALTSVAAILALAGG
                     EWFGWNWLDPMTGLAGSLIIGVWARGLLIETGRVLLDREMDDPLVGRVRAALEDDSGT
                     RVEDLHLWRIGRRQYACIVAVSDALRLAPATYKARLAGFPQIVHVTVEVNERGLS"
     gene            13579..14241
                     /locus_tag="PK144_02945"
     CDS             13579..14241
                     /locus_tag="PK144_02945"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020302797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNF64712.1"
                     /translation="MEPSFWHDKWERRDLGFHQSEINPLLQRHWPRLGLAAGAGVFVP
                     LAGKSLDLRWLAAQGHPVLGVELSETACTEFFTDSGLPFERSHDGPFERFRGAGIELL
                     CGDFFALQAAHLAGVAAIYDRAALIALPPPMRRRYVAHLQALCPPLPTLLITLDYEQA
                     ERDGPPFSVPESEVRALYEPHWPVRCVERVDLLASEPRYRERGMSRIEDCAFVLGALA
                     GG"
     gene            complement(14246..14884)
                     /locus_tag="PK144_02950"
     CDS             complement(14246..14884)
                     /locus_tag="PK144_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF64713.1"
                     /translation="MSRPLPARDEVWSAEDYLGWPSDTPRCELIDGRVYAMSPAPTLD
                     HQRITGALFAALRSTLAERRGRGSGGAGCGCEVLMAPVDVRLDAHTVVQPDVLVVCDP
                     AKLANGRHVDGAPDLVVEVLSPSNALKDRRLKRRVYERAGVPELLLIDPEQRYVEIHR
                     LGSDGRYAAPELIAPQEPLLLALLPDWQPTPGELFGWPPADLSLDEDMPAAP"
     gene            complement(14964..16127)
                     /locus_tag="PK144_02955"
     CDS             complement(14964..16127)
                     /locus_tag="PK144_02955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF64714.1"
                     /translation="MPPRPPPGFHERRRGDRRVLQFSESPGWIGSAGLRPASWAEMRV
                     QFLTRFLFVSIALFYFSGRPGEQPFWYPVWAVWLHVGSYWLLNAVNMWHAWLGRYGES
                     RIRLAMVLDVASISLFLVNDPYSVPPSLLAYLMVILGNGMRYGLRMYGESLAWSFGCA
                     LLALVVRYQDSLTDLRAGLIFLPLFSAIIVMYAYFLLLRLEQARVQLERVSRYDGLTG
                     LLNRRGLAESVELLQRLMDRDTDRITVVFADMDRFKSVNDTLGHTAGDAVLQEIGRIL
                     REGVRRSDLIARYGGDEFVFVLPHTGREQALQVTGRVERRIHELSRRTGIDFGITFGM
                     GETGAGHRADVLELIDAVDREMYEAKRARRQQRETTLRADAAPAWDTPAAGGG"
     gene            16342..17994
                     /locus_tag="PK144_02960"
     CDS             16342..17994
                     /locus_tag="PK144_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HNF64715.1"
                     /translation="MGVPIRASRLAAVVTLALAGSAAFAADHNDPNAINSIFSDIPVS
                     PADLYDFFGFPADDRSQGEKVVMALTFASVPKTGVLDPDLLYRIRIHPEARLVRPSKE
                     DWSLDTVLAYAGALKDRYLDSRTAEVRVRVDARQQAKVDFIGFPSGSFTKIIPTNTPV
                     SVQAPDGQALQVFVGGRDDAFFNDLPGFFRSINYGPQFYLIPLGADPDLREFAIPKTL
                     LELEGNTLFNFDPAHPRLGEGEKLPLPDSGPRELQGVPYRKDANGNYRFVYSGRDAQA
                     GRNVNAIILELPLGYVTDAPDKNRVVSAWGESWVLKAANKVETIPDEQKGFGPWLEKR
                     LPQGERSEFERELERYKRVDTDGVPFEDAALSEREDSRQLGADNLSLARRFVIRFAHL
                     GWGFGPSIDALGLRTCFDHDHSPVSIHRYDRLAIEAFPRAKTCFFQEVNMPDASWNPR
                     GLPIPLKRTFEIFIPNVSGIDMDTNGSWPYGRRLEDQVATRFLSLFLDMKTGCGGKPC
                     NVETLSQQALWDGAPIEPKTPPNPLHNDKPFLKQFPYLADPW"
     gene            18305..18583
                     /locus_tag="PK144_02965"
     CDS             18305..18583
                     /locus_tag="PK144_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNF64716.1"
                     /translation="MSTLNLSKTERIDVRASTPVKQLLQEAARACHKSVSEFLLDAGV
                     TAAAQTLADRRQFVLDDAQWQAFQEALDRPVQSKPRLKKLLREPGLLG"
     gene            18580..19083
                     /locus_tag="PK144_02970"
     CDS             18580..19083
                     /locus_tag="PK144_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64717.1"
                     /translation="MSHGYAAVRKLAATDQVDAFDCGQAALNQFLQRYALVNQKAGSA
                     QTYVCCRDDVVVGFYSLVVGSVDPEAAPSRVMKGLARHPVPVMILARLAVDTQHQRKG
                     LGQALLRDALLRTAQAADIAGIRCLLIHAKDDAARRWYESWEFEPSPSDPYHLFLMIK
                     DINGLLS"
BASE COUNT         3110 a         6552 c         6674 g         3006 t
ORIGIN      
        1 gttgctgctg caggaagatc tgccgggggg tggatgtcag caggtcggcc ccgcgcacga
       61 cgtgcgtgac gccctgctcg ccgtcgtcga cgacgaccgc cagcgcatag gcgatcaggc
      121 cgtcggcgcg gcgcagcacg tggtcgccga cctctcggcc gaggcactgc gcctgcgggc
      181 cttgcagccg gtcggtgaaa ccgaccgtca tcgcgtcgtc gatgcgcagg cgcagggccc
      241 gtcccggcgc ctgcgcgtgt ccggcgttgc ggcagaaccc cgggtagacg agtccggcag
      301 ggccgctggc tgcgccggag gctgcgaggc gggcgcggct gcaggtgcag gcatacagcc
      361 ggccggccgc ctgcagttgc gccagcgcgg cctcgtacac ggccgtgcgc cggctctggc
      421 gcagcacctc gccgtcccag tgcaggccgt ggcgctcgag atcggcgagg atcagcgctt
      481 cggccccggc caccgtgcgc gggccgtcga catcctcgat gcgcagcagc cagcgtccgc
      541 cggcggcgcg ggcgtcgagc cagctcgcga gcgccgtgac cagcgagccg aagtgcagcg
      601 gcccgctggg cgagggggcg aaacggccgg tgtaaggcga catcgtgtgt tgtgtcattt
      661 cgtaaaggat tgcgctggcg gatcatgccg gatgccggcc ggaggatgcc caggcactcc
      721 ggccgtgcca tccccttgat cccagctgct gcgtttttgc ggagccagcc ccatgaccat
      781 ccagtcactg cccctgctgc atgtgatcga accgatcccg gccagcgatt cggcgcttgc
      841 ggccctgaac ctgatgctcg atctggccat caaccacctg ccggtggtcg atgcgcacgg
      901 gcattacctc ggcatgctga cgctgaccga cctgctgcac gagatcctgc cggccggctt
      961 gcggctcggt catggcatcg gccagcccga cgtgagcttt ctcggcgatg ccgcctcgct
     1021 gctgtcgacg cacctcggcc agctctcggc aaggaccgcc ggggacgtcg ccgatccgca
     1081 cgtggtgccg ctcgaaaccg actgcccgct ggtcgaggcg gcgcgccggc tggccgaggc
     1141 gcgcaatccg ctgccggtcg tcgacgccga tcgccacatc gtcggcgtgc tcagcgtgcg
     1201 gctgctgctg acccatctgc tcggccgggg agtgcagaac gatgcacggt gaaccggcct
     1261 ccgtctccca cgtgatcttc gggctgccgc cgctgctggt gtcgctcgcg atcttcgtcg
     1321 ccacctacgc gatcgtcatt tccgaaaagc tcaaccgcgc cgtgatcgcc gcgctcgggg
     1381 ccgggctgat gatcctctcc ggcgtgctga cgcagctcga agccgtgcac ggcatcgatt
     1441 tcaacaccat cggcctgctg acggcgatga tggtcatcgt cgcgatcacg cagaagaccg
     1501 gcgtgttcca gtacgtcgcg atcgtcgccg ccaagcgcgt gaaggccgat ccctggggac
     1561 tgctggtgat gatggcggtg gtcacggcgg tgttctcggc cttcctcgac aacgtcacga
     1621 ccgtgctgct ggtgacgccg gtgatcctgc tgatcaccga ggcgctcggt gtgccgccgt
     1681 atccgtatct gttcacggtg atcttcgcgt cgaacatcgg cggcaccgcg acgctgatcg
     1741 gtgacccgcc gaacatcatg atcggctcgg ccgccaacct gagcttcaac accttcctgc
     1801 tgcatctgac gccggtggtg ctcgtcgtgt tcgcggcgac gctgctgccg atctggctgg
     1861 tctggggccg cacgctgcgg gcgacgccgg aggcgcgcgc ccaggtcatg gccttcgacg
     1921 agaaatcggc gatcacggat ccgcggctgc tgaagcaggc gctggcggtg atcggtgcgg
     1981 tgatcttcgg cttcatgatc gcgcatccgc tgcatctgga gccggcgacg atcgccggtt
     2041 tcggggctgc gctgctcttg gcgctgcagg tcgtgcgcca cgatgccgag caccagaccg
     2101 aggaggtcgc gcacgcgctg cgcgagatcg agtgggtgac gatcttcttc ttcgtcggcc
     2161 tgttcgtcgt cgtcaccggc atcgagaagg ccggcgcgct gaagctgatg gccgactgga
     2221 tcgttgcggc cacgcagggg gactacaagg ccacggcgat ggcgatcctg tgggtgtcgg
     2281 ccgtgctgtc ggcggtggtc gacaacattc cgttcgtcgc gacgatgatc ccggtgatcc
     2341 agaacatggc gccgaccttc ggggccgaca acctgctgcc gctgtggtgg gcgctgtcgc
     2401 tcggggcctg tctcggcggc aacggcacgc tggtcggagc cagcgccaat ctggtggtcg
     2461 ccggcttcgc cgagcgtgcc ggccagccga tccgcttcaa gaccttcctg ctgatggcgt
     2521 ttccgctgat gctgatgagc atcgcgatca gcacggtcta tctgtacctg cgctatctct
     2581 gaacccgcat ccgcccggca cggctgacgg tcgtgccggg cgggcggggc gatcgggggg
     2641 cggtcaggaa atcagtcgtc gtcgcgcatc accagttcgg gccggtcgcc gtaacgctgg
     2701 tagaaggcct tcagcgcgaa cggtggcagc agcgtcgaca gcgcgacgac gatcagcagc
     2761 accgcgtaca gctcctggct caggatcgcc gccccgagtc cgagcttcgc gaagatcagc
     2821 ccgacctcgc cgcgcggcac catgctcagg ccgatcgcgg cacggcgcag cgtcggttcg
     2881 gcgatgaacc agccgctcgc caccttgccg gcgactgcca gcacgaccag cacgccggcc
     2941 atcagccaga cgcgcgccga ggaccagtcg acggccgaca ggtccagcga cacgccgacc
     3001 atcacgaaga agatcggcgt gtaggtgtgc agcagcggcc ggaactgctt ttcgagccgg
     3061 tgcgtgagct ccggacgcgg cgcgaaagcg cgctccagcg tgcgccggaa cggcaggcgc
     3121 aggcgctgcg tgatcgacat ccggaagtgc tggctcagcg cgatgccggc cgcgaagccg
     3181 cccatgatcg ccggagcgcc gaccgcgtgg gcgagccacg agaacagcat gatcaggctg
     3241 atgaccagcg tgatcagcag gccctcggat tcgagcttgc ggtcggtgtg atcgatcgcc
     3301 caggcgatga tcttgccggc gatcggcgcg agcaccatga acacgacgat gaacagcgcg
     3361 atgctggcgg tgctcgccag cgacacgccc ttcggcgacg agaagtcgta caggaaggcg
     3421 aggatgatga cgccgaggat gtcgtcgatg actgctgcgc cgatgacgat ctgcgcctcg
     3481 tcgctgcgcc ggcgcatcag gtcgtcgagc acgcgcacgg tgatgccgat gctggtggcg
     3541 gtcagcgtgc cgccgatgaa cagcgcggcc aagatcgagg tgtcgaacag cagccagccc
     3601 gcggcaaatc cgagcacgag cggcaggaag gcaccgacgg cggcgacgac gaagggcttg
     3661 cggccggctt cggcgagccg gaacaggttg gtgtcgaggc cgacctcgaa cagcagcagg
     3721 atgatgccga cctcggcgag cgtcatcagc gtctcggtcg gctgcaccca gccgagcacc
     3781 gaggggccga gcaccaggcc ggcgagcagt tcgccgatca cggatggggc gccgaggcgg
     3841 ctggccagct cgccgaagag gcgggcggtc agcagcagca ccgccagcat caggaagaat
     3901 tcgtggattg ccattgcttc tccgggcctc gttgcagggg gcggtgcgct gccgcccgta
     3961 ggggggttgg gcagtgtcgc ggctcgaatc cggcttggca attcttacgc gtaagaagaa
     4021 acggataccg tgttgattgt ctcgctatct cgcgcaatcg cgccatgaaa aacggggtgc
     4081 ggccgcgctg gcgaccgcac cccgtccgtt caggctgttc aggcggattc agcagtgaat
     4141 ccgcagatca ggcggcttcg ccggccagga acatccaggt ctcgacaacg gtgtccgggt
     4201 tcagcgacac gctctcgatg ccttcctcca tcagccactt ggccagatcc gggtgatccg
     4261 acggaccctg gccgcagatg ccgatgtact tgccgttgcg ccggcaggcc tggatcgcca
     4321 tcgacagcag cttcttgacc gccggattgc gctcgtcgaa caggtgcgcg atcaggccgg
     4381 agtcgcgatc gagtccgagc gacagctggg tcaggtcgtt cgagccgatc gagaagccgt
     4441 cgaagtattc gaggaactca tcggcgagca gcgcgttcga cggcagttcg cacatcatga
     4501 tcagcttcag gccgtcccgg ccgcgttcca ggccgttggc ggcgaggatc tcgactacct
     4561 gacgcgcttc ctccagcgtg cgcacgaacg ggatcatgac ctggacgttg tccagaccca
     4621 tgtcgttgcg caccttcttc atcgcccggc attcgagctc gaagcagtcg cggaagctct
     4681 ccgagacgta ccgcgaggcg ccgcggtagc cgatcatcgg gttttcctcg tgcggctcgt
     4741 acagcgggcc gccgatcagg ttcgcgtact cgttggactt gaagtccgac atgcgcacga
     4801 tgaccggctt cggcgcgaag gccgcggcca gcgtcgcgat gccttcggcg agcttgtcga
     4861 cgtagaagtc gaccggcgag gcgtagccgg cgatctgcga gtcgacgcgg accttgatgt
     4921 cggagggcag cgcgtcgtag ttcagcagcg ccttcgggtg cacgccgatc atgcggttga
     4981 tgatgaactc cagccgcgcc agaccgacgc cggcgttcgg cagccgcgag aagtcgaagg
     5041 cgcggtcggg gttgccgacg ttcatcatga tcttgaacgc gagctgcggc atcgcgtcga
     5101 ggctgacctc gcggatgtcg aagtcgatca ggccgcggta gatgtagccg gtgtcgccct
     5161 cggcgcagga gaccgtcacc ggttcgccgt cgcgcacgcg ctcggtggca tcgccgcagc
     5221 cgaccaccgc cgggatgccg agttcacggg cgatgatcgc cgcgtggcag gtgcggccgc
     5281 cgcggttggt gacgatggcg gaggcgcgct tcatgatcgg ttcccagtcc gggtcggtca
     5341 tgtcggtcac gagcacgtcg ccggcctgga ccttggccat ctccttgatg tcgctgatca
     5401 cgcgcacgac gcccgagccg atgcgctggc cgatcgagcg gccggtggcg atgacctcgc
     5461 cgcggcgcga cagctggtag cgctgcatga cctgcgagtc ggcgcggctc ttcacggtct
     5521 ccgggcgggc ctgcaggatg tagagctggc cgtcggtgcc gtccttgccc cattcgatgt
     5581 ccatcggccg gccgtagtgg cgctcgatga tcagcgcctg acgggcgagt gcctcgacct
     5641 cggcatcggc aagcgcgaag cggcggcgct cggcgagttc gacatcgacg gtcttgacgc
     5701 tgcgtccgac cgtctgctcg gcgctgttgt agaccatctt gatggccttg ccgccgaggt
     5761 tgcggcgcag cacggccggg cggccggcgg cgagcgtcgg cttgtgcacg tagaactcgt
     5821 cggggttcac ggcgccctgc acgacggtct cgccgaggcc gtaggaggcg gtgatgaaca
     5881 ccgcgtcgtc gaagccggat tcggtgtcga gcgtgaacat gacgccggcg gcaccgaggt
     5941 cggagcggca catcttctgg atgccggccg acagcgcgac ctcgccgtgc tcgaagccgt
     6001 ggtgcacgcg gtaggcgatg gcgcggtcgt tatataagga ggcgaacacg tgcttgatcg
     6061 cagccatgac ctgatcgagg ccgcgcacgt tcaggaaggt ttcctgctgg cccgcgaacg
     6121 aggcatcggg cagatcctcg gcggtcgccg aggagcgcac cgcgaccgac agctcggcac
     6181 cgtagcgcga tgtcatgtcg cggtacgcgg tttcgatcgc gtcgagcagc gccggctgga
     6241 acggcgcatc gatgacccac tggcgcacgc gcgcaccggt ctcggccagc gcgttcacat
     6301 cctcgatgtc gagcttcgcc agttcggcgt tgatgcggtc gatgatgccg ttgacggtca
     6361 ggaactcgcg gaaggcctcg gccgtggtcg cgaacccgcc cggtacctgg acgccggcag
     6421 cagacagatg gctgatcatc tcgccgagcg acgcgttctt gccgccgacg cggtccacgt
     6481 cgcccatgcc gagggcttca aaccaaacga cgtactctgc caatgtagtt tcctcttcgg
     6541 aacgcattta aaagcctggg aaagccggct gcgcaggtac cgggaccgcc gtgccgatgc
     6601 gtacggcgct gtcctggtga cccggcacga aagcccgggc cgcgcgaggc gcgcgattat
     6661 aatgtcgccc ccacagggac ggaacggatt tcggcatcca tctcggggat gccggcgcgg
     6721 ctgtatccag tcctcacaaa agcacggcgg gaccacgcgt tggtgaatct gatctttccg
     6781 gtccgggccc acgccggcct cgatccgcgt cccggcagct tctcgggcct gatggacctc
     6841 tacgagcgca actacatgct gctgcgccgg ctgctgccgg ccatgctgcc ggcagatgcg
     6901 agacaggtct cgcaggcagc cggggcgctc gacctgcacc tgcacgtcag cgagcgcttc
     6961 cgctacacca gcgagctgtc cctgacctac cgcttcgagc acggcgacgg ccaggtgctg
     7021 ctggagccgg acctgcacgt gcgcatctat cacgatgcgc ggctcgccga ggtcatggtc
     7081 gcgcggttgc gccacgcacc gccgtaccgg ctcggggccg ccgaggaggc gagcgagctg
     7141 tacgcgcgct gccgggccaa ccgttttttg tacaaatggc tgtgttactg cacgcgccag
     7201 gggcacggtt tcgcgtccgc caccggtgca ccgcacaata gttgatcgaa tcgcttgggt
     7261 accgtagcat cgaaaccaga ctgcgccgaa ggtcacccgg gccggcggtg cgcaagacgg
     7321 ctgcggaggt actcatgaag ctttcgacga aagggcgcta cgcggtgacg gcgatgatgg
     7381 atctggcgat ccatgattca cacggaccgg tgacgctggc ggacatctcg cagtgccagg
     7441 gtatctcgct gtcctatctg gagcagctgt tcgcgcgtct gcgtcgctcg ggactggtcg
     7501 agggcgtgcg cggacccggt ggtggttacc ggctggcgcg gcgtgccgat gcgatcagcg
     7561 ttgccgacat catcgccgcc gtcgacgagc gcgtggatgt gacccgctgc caggggcgcg
     7621 gcgactgcga caacggccgc cagtgcctga cgcacgatct ctggaatgac ctgagcgagc
     7681 gcatcgcgac cttcctgacc ggcatcacgc tcgccgattt cgtcgaccgc ccggcggtgc
     7741 gcgagatggc cgagcgcaac gcgctgcgcc agacgcaggg ccacggccac ggccagactc
     7801 aggcccggcg ccagctgacg gtgcgggagc gccgcatcag cgcctgaagc cgcaagtcct
     7861 gcctccgcgt tcacgacgcc cccgtctgcg acgggggcgt cgtgctgtcc gggcataaaa
     7921 aaagcgggga ccgaagtccc cgctcaagat aaacctgcct caggagatca aacgagcttg
     7981 tcgcccggag ccgcccgcga gaacggctcc gccgtgaagc tcaaggtcac acaccgccat
     8041 tcctcaatgc gaccttgagg cgtattgaac gcccgggctc gcgcaggcgc gttgatctgc
     8101 atcaagccgg cgtgtgacgg cagcgcgggc tcagcccgcc gggcggttgt cgaccatgaa
     8161 ggtcgccttg ccctcgacga ccaccttgcc gtgcaccgtg cagacggtgt cggcgtgcac
     8221 gcggcgcttg gcggcgttga tctcggtgac ggtgacggtc gcggtgaccg tgtcgccgat
     8281 gcgcaccggc gccttgaact tcagcgactg ctcgatgtag atcgcgcccg gcccgggcag
     8341 ccgcgtgccg gcgaccgtcg agatcagccc gccgcagaag atgccgtgca cgatgcgacc
     8401 cttgaacatc gtcgtttcgg caaattcctg gttgatgtgc accgggttgt cgtcgccggt
     8461 gatgccggcg aacaggatca cgtcggcttc ggtgacggtc ttcgcgtagg acgcgctctg
     8521 gccgacgctc aggtcttcga tgtagtagcc gtgcagatcg gacatggtca agcagcctcg
     8581 ggggtgggga gggagagggc aggacagggt aagaaaaccc gggcccggcg catagactgt
     8641 gccggctcgc cccggacgat cccggcatct tgcgacggca ctgggtaatc ggtaaagtcg
     8701 tactagggtt ttcccttagt gctcattccg ataataccgg tatccagcgt cgccgatcgc
     8761 ggccgggccg gacgggaagc cgcatatatc ccaccgaggt accgcatcga tgaaagtcct
     8821 ggtcgccgtc aaacgggtgg tcgattacaa cgtcaaggtg cgtccgaaag cggacggcac
     8881 cggcgtcgag actgccaacg tcaagatgtc gatgaatccc ttcgacgaga tcgccgtcga
     8941 ggaagccgtg cgcctgcagg aggccggcaa ggccagcgag gtcgtcgtcg tctcgctcgg
     9001 cgtttccgcc tgtcaggaca cgctgcgcac cgcactggcc atgggcgccg atcgcgcgat
     9061 cctggtcgag accgacgccg aactgcagcc gctcgcggtc gcgaagctgc tgaaggcggt
     9121 ggtcgacaag gaacagcccg gcctcgtgat cctcggcaag caggcgatcg acgatgactg
     9181 caaccagacc ggccagatgc tcgccgcgct gctcggctgc ggccagggca cgttcgcgtc
     9241 gaaggtcgag cctggcgacg gcctcgtcac cgtcacccgc gaggtcgatg gcggtgccga
     9301 gaccgtcgag ctgaagctgc cggccgtcat caccagcgac ctgcgcctga acgagccgcg
     9361 ctacgtcaag ctgccgaaca tcatgaaggc caagaagaag ccgctcgaag tgctgaagcc
     9421 cgaggcgttc ggcgtcgacg tgacgccgcg gctgaagacg ctgaaggtcg ccgagccgcc
     9481 caagcgcgcc ggcggcgtca aggtggcctc ggttgccgaa ctggtcgaca agctcaagaa
     9541 cgtcgcgcag gtgctctgat catggccgtc ctcgtcattg ccgaacacga taacgcggtg
     9601 ctgaaggccg cgacgctgaa caccgtcacc gccgccgccg gactcggcga ggtgcacgtg
     9661 ctggtcgccg gccagggttg cggcgcggtt gccgaggccg ctgcgcaggt cgccggcgtc
     9721 agcaaggtgc tgcttgccga cgacgccgcg ctcgcgcatg cgctggccga gaacctcgcg
     9781 ccgctggtca ccggcctcgc cggcggctac agccacgtgc tggcgccggc caccgccgcc
     9841 ggcaagaacg tcgcaccgcg catcgccgcg ctgctcgacg tggcgccggt ctccgacatc
     9901 atcgagatcg tctcgcccga taccttcgtg cgcccgatct acgccggcaa cgtgctcgcc
     9961 accgtgcagt cggccgaccc ggtcaaggtc gtcaccgtgc gcggcacggc cttcgcggct
    10021 gcggcagcga gcggcggcag cgcgccgatc gagcgcatca gcggcgccgc ggacagcggg
    10081 gtcgcgcgct tcgtcggcca ggagatcatg aagtccgagc gtcccgagct caccagcgcc
    10141 cgcgtcgtcg tctccggcgg ccgcggtctc ggttcgggcg agaacttcgc gatcctcgaa
    10201 gccctggccg acaagctcgg cgccgcggtc ggtgcctcgc gcgccgccgt cgatgccggc
    10261 ttcgtgccga acgactatca ggtcggccag accggcaaga tcgtcgcgcc gcagctgtac
    10321 atcgccgtcg gcatctccgg cgcgatccag cacctcgccg gcatgaagga cagcaaggtc
    10381 atcgtcgcga tcaacaagga cgaggaggcg ccgattttcc aggtggccga ctacggcctg
    10441 gtcggcgacc tgttccagct cgttccggaa ctgacgcaga gcctctgatc accggcatat
    10501 cccggatcac ccggcagtac cccggcagga gccgcaccgc ccgtccgttg cggcgcggcc
    10561 cctgccggca ttcgacaaga caggagtgag gagtcatgag cagctatacc gcccccgtcc
    10621 gcgacatgcg cttcgtcatc ggtgaactcg tcgacctcga cgccatcgcc gccctgccgg
    10681 gctgcgagga cgtcggtccc gacctcgtcg aggcggtgct ggaggaggcg ggcaagctcg
    10741 ccggcaacgt cctcgatccg ctgaaccagc aggccgaccg caacggcgcg cgctggaccg
    10801 aggccggcgt cgtcgccgcc cccggtttcg ccgaggccta ccagcagttc atcgaaggtg
    10861 gctggaacgg tctggcctgc ccgaccgaat acgatggcca gggcctgccg gaactgctcg
    10921 gcaccgccac gcacgagatg tggaacgccg cgaacatggc cttcgcgctg tgcccgatgc
    10981 tgacgctcgg cgcaatcgag gccatccagc actacgcgac cgacgagctg aaggccgcgt
    11041 acctcgcgaa catggtcgcc ggcacctgga ccggcacgat gaacctgacc gaaccgcagg
    11101 ccggctcgga tctggccgcg gtgcgcagca aggccgtgcc cgaaggggat cactaccgca
    11161 tcagcggcca gaagatcttc atcacctggg gcgatcacga catgaccgac aacgtcgtgc
    11221 atctcgtgct cgcgcgcctg cccgacgccc cggccggcgt gcgcggcatc tcgctgttcc
    11281 tggtgccgaa gttcctgctg aacgccgacg gcacgccggg cgagcgcaac accgtgcagt
    11341 gcgtgtcgat cgagcacaag ctcggcatcc acggcagccc gacctgcgtg ctcgccttcg
    11401 aggaagcgat cggctatctg gtcggcaccg ccaacaaggg cctcgcgcac atgttcacga
    11461 tgatgaacga ggcgcgcctg aaggtcggcc tgcaggggct cgcacaggcc gatcgggcct
    11521 accagcaggc gcgctggtac gcgaatgacc gcatccaggg ccgcaccgtc gtcgatcagg
    11581 gcgatgccag gtcgccgatc atcaaccatc cggacgtgcg ccggatgctg atgacgatga
    11641 gggcgcaggc cgaggccatg cgcgcggtcg cctacaccac cgccgcgcag gtcgacatcg
    11701 cccgccgcca tcccgacgag gccgtgcgca aggccgcgca ggcgcgcgtc gacctgatga
    11761 tcccgatcgt caagggctgg tctaccgaga ccggtcagga cgtgacctcg atcggcatcc
    11821 aggtccacgg tggcatgggc tacgtcgagg agaccggtgc ggcgcagtac ttccgcgatg
    11881 cacgcatcac gacgatctac gagggcacga ccgcgatcca ggccaacgac ttcgtcgggc
    11941 gcaagctggc gctggatcgc ggcgcggcga tgcaggcgct gctcgccgac atgcgcgcga
    12001 cgctcgccgg tctcgatgcc gccgatggcg aggacctggc cgtgatccgc acccggctcg
    12061 ctccggccgt cgatgcgctc gaagaggctg cggactgggt ggtcgcaaat tacgaggcct
    12121 gccaggccga cgtgctcggg gccgccttca acctgctgat gatggccggc tgcgtctgcg
    12181 gcggctggca gatggcgcgg gccgcgctgg cggcgagtgc gcagctcgcg gccggcgggg
    12241 atccgaagtt ctacggcgcc aagctcgtga ccgcgcgctt ctacgccgaa cagctgctgc
    12301 cgcgcgccgg cgcgctgctg accgtcgcca aggcaggtgc ggcatcgatg atggcgctcg
    12361 acccggagca gttctgatcc ggtccggttt cctttcgtag tgaaccctcg gtcgacaggg
    12421 ctcgggggcg accttcgggt cgcccccgtt ttttttgggg cggcttgtcc ggcctgcccg
    12481 gcggcggccc ggtcaggaca ggccgcgctc gttgacctcg acggtcacgt gcacgatctg
    12541 cgggaagccg gcgagccggg ccttgtaggt cgccggggcc agacgcagcg catcgctgac
    12601 ggcgacgatg caggcgtact ggcggcggcc gatgcgccag aggtgcaggt cctcgacgcg
    12661 ggtgccgctg tcatcctcga gggctgcgcg cacgcgcccg accagcgggt cgtccatctc
    12721 gcgatcgagc agcacgcggc cggtctcgat cagcagtccg cgtgcccaga cgccgatgat
    12781 caggctgccg gccagcccgg tcatcggatc cagccagttc cagccgaacc actcgccgcc
    12841 ggccagcgcc aggatcgccg cgaccgaggt cagcgcatcg gcgagcacgt gcaggtaggc
    12901 ggcgcggtgg ttgagatcat ggccgtgcgc atggggctgt ccggcctcgt ggcagccggc
    12961 agcgtcgtgc ccgtgcccgt gcccgtgccc gtgcgcatgg gcggcctcgt gcgcgtgcgt
    13021 cccgtgctca tggccgtcgt cgtgcagcag ccaggcgctc gcgaggttga cgagcaggcc
    13081 gatggccgcg acgcccatcg cctcgccgaa ggcgatgtcg acggggtttg cgagccgcag
    13141 cagcgattcg aaggccatca gcaccgcgac cgcggccagc agcaccgcgc tcgcgaagcc
    13201 gccgagcacc tcgaccttcc aggtgccgaa ggcgtagcgg tgatcgccgg cgtggcggcg
    13261 cgcatggcgg taggcaaggg cggccagccc gatcgcgacg acgtgcgagg ccatgtgcca
    13321 gccatcggcg agcagcgcca tcgaacccga ccaccagccg gcgaggatct cgacgagcat
    13381 catcaccgcc gtcagccaga cgacgtgcca ggcgcgctgc tcggcctgcg gtgcgctgct
    13441 gtggaaggcg tggctgtgcg cgcacggcgg ggcggcgggc tcggtcatgg cgatcacgca
    13501 tcgggcaggc gggagggccg agggtacgcc gccgccgctt atcatcggca gccgccatcc
    13561 tgcctcggga gatgactcgt ggaaccgtcc ttctggcacg acaaatggga acgccgtgat
    13621 ctcggcttcc atcagtccga aatcaatccg ctgctgcagc gccactggcc gcggctcggt
    13681 ctggcggccg gcgcgggcgt gttcgtgccg ctcgccggca agagcctcga tctgcggtgg
    13741 ctggccgctc aggggcatcc ggtgctcggc gtcgagctgt cggaaaccgc gtgcaccgaa
    13801 ttcttcacgg acagcggcct gccattcgag cgaagccacg acggcccgtt cgagcgcttc
    13861 cggggcgcgg gcatcgagct gctgtgcggg gacttcttcg cgctgcaggc cgcgcatctg
    13921 gccggtgttg cggcgatcta cgaccgcgcg gcgctgatcg ccctgccgcc gccgatgcgc
    13981 cggcgctatg tcgcgcatct gcaggcgctc tgcccgccgc tgccgacgct gctgatcacg
    14041 ctcgactacg agcaggccga gcgcgacggc ccgccgttca gcgtgccgga gtccgaggtg
    14101 cgcgcgctgt acgagccgca ctggccggtg cgctgcgtcg agcgcgtcga tctgctcgcc
    14161 agcgagccgc gctatcgcga gcgcggcatg agccgcatcg aggactgcgc cttcgtgctc
    14221 ggagcgctgg cgggcggctg agcggctacg gcgccgccgg catgtcttcg tcgagcgaca
    14281 ggtcggccgg cggccagccg aacagctcgc ccggtgtcgg ctgccagtcc ggcagcagcg
    14341 cgagcagcag cggctcctgc ggcgcgatca gctccggagc ggcgtaacgg ccgtcgctgc
    14401 cgaggcggtg gatttcgacg tagcgctgtt ccgggtcgat cagcagcagt tcgggcacgc
    14461 cggcacgctc gtagacgcgt cgcttcagcc gccggtcctt cagcgcgttc gacggcgaca
    14521 gcacctcgac gaccagatcg ggtgcgccat cgacatggcg gccattggcg agcttcgccg
    14581 ggtcgcagac gaccagcacg tcgggctgca cgacggtgtg ggcgtcgaga cgcacgtcca
    14641 cgggggccat caacacttcg cagccacagc cggcaccgcc ggaaccacgt ccgcggcgct
    14701 cggcgagtgt cgatcgcagc gcggcaaaca gggcaccggt gatgcgctga tgatccagcg
    14761 tcggtgccgg cgacatcgca tacacgcgcc cgtcgatcag ctcgcagcgc ggcgtgtcgc
    14821 tcggccagcc gagatagtcc tcggcgctcc agacttcgtc acgggcgggc agcgggcggg
    14881 acatggtcgg gtacacctgc ggagtcggcc gaagcgggat ggcgcgatgc taggccgccg
    14941 ccgcgcagcc ggccagtggc gtctcagcca ccgcccgcgg cgggagtgtc ccaggccgga
    15001 gccgcatcgg cccgcagcgt ggtctcgcgc tgctggcggc gtgcgcgctt ggcctcgtac
    15061 atctcgcgat cgaccgcgtc gatcagttcg agcacatcgg cgcgatgccc ggcaccggtc
    15121 tcgcccatgc cgaaggtgat gccgaagtcg atgccggtgc gccgggaaag ctcgtggatg
    15181 cgccgctcga cccggccggt gacctgcagc gcctgttcgc ggccggtgtg cggcagcacg
    15241 aacacgaact cgtcgccgcc gtagcgggcg atcaggtcgg agcggcgcac gccctcgcgc
    15301 aggatgcggc cgatctcctg cagcaccgcg tcgccggcgg tgtggccgag cgtgtcgttg
    15361 accgacttga agcggtccat gtcggcgaac acgacggtga tgcggtcggt gtcgcgatcc
    15421 atcagccgct gcagcagctc gaccgattcg gcgaggccgc ggcggttcag caggccggtc
    15481 aggccgtcgt agcggctgac gcgttcgagc tgcacgcgcg cctgttcgag gcgcagcagc
    15541 aggaagtacg cgtacatgac gatgatcgcg ctgaacagcg gcaggaagat cagcccggcg
    15601 cgcaggtcgg tcagcgagtc ctgatagcgc acgacgagcg cgagcagcgc gcagccgaag
    15661 ctccaggcca gcgattcgcc gtacatgcgc agcccgtaac gcatgccgtt gccgaggatg
    15721 accatcagat aggcgagcag cgacggcggc acgctgtacg gatcgttgac caggaacagc
    15781 gagatgctgg ccacgtcgag caccatcgcc agccggatgc gcgattcgcc atagcgcccg
    15841 agccaggcgt gccacatgtt cacggcgttc agcagccagt agctgccgac gtgcagccac
    15901 acggcccaga ccggatacca gaacggctgc tcgcccggcc ggccgctgaa gtagaacagc
    15961 gcgatgctca cgaacagaaa gcgcgtcagg aactgcacgc gcatctcggc ccacgaggcc
    16021 ggccgcaggc cggcactgcc gatccagccc ggggattcgc tgaactgcag cacgcggcgg
    16081 tcgccacgcc ggcgttcgtg gaaaccgggc ggggggcgtg gaggcaagcg cagctccttg
    16141 gcagcaggtt cgaccgggca agcatatacg acggatgccg tatgcagcga agggccatgc
    16201 cgcccgtgca agcccttgag cggacgcggc aagtcgccgt tgccttgcgc ggggtgcggc
    16261 tctgctatac cgcaggcacg ccgacggacg gcgatgcgaa ccgcccgcgg cgcctcaccg
    16321 gaccctcagg agggacagga catgggtgtt cccatccgcg ccagccgtct ggccgccgtc
    16381 gtcacgctcg ccctggccgg atcggcggcc ttcgcggccg atcacaacga tccgaacgcg
    16441 atcaattcca tcttctcgga cattccggtc agcccggccg acctgtacga cttcttcggc
    16501 tttccggccg acgaccgcag ccagggcgag aaggtcgtga tggcgctgac cttcgcgtcg
    16561 gtgccgaaga ccggcgtgct cgatcccgac ctgctgtacc gcatccgcat ccaccccgag
    16621 gcgcggctgg tgcggccgtc gaaggaggac tggagcctcg acaccgtgct cgcctacgcc
    16681 ggcgcgctga aggatcgcta tctcgacagc cgcaccgccg aggtgcgcgt gcgcgtcgat
    16741 gcccgccagc aggccaaggt cgatttcatc ggcttcccgt ccggcagctt cacgaagatc
    16801 atcccgacca acacgccggt gagcgtgcag gcgccggacg gccaggcgct gcaggtgttt
    16861 gtcggcggtc gcgacgatgc cttcttcaac gacctgccgg gtttcttccg ctcgatcaac
    16921 tacgggccgc agttctacct gatcccgctc ggtgccgatc cggacctgcg cgagttcgcg
    16981 atcccgaaga cgctgctcga actcgaaggc aacacgctgt tcaacttcga tccggcgcat
    17041 ccacggctcg gggagggcga gaagctgccg ctgcccgaca gcggcccgcg cgagctgcag
    17101 ggcgtgccct accgcaagga cgccaacggc aactaccgct tcgtctacag cggcagggac
    17161 gcgcaggccg ggcgcaacgt caacgccatc atcctcgaac tgccgctcgg ctacgtgacc
    17221 gacgcaccgg acaagaaccg cgttgtcagc gcctggggcg agagctgggt gctgaaggcc
    17281 gcgaacaagg tcgagaccat cccggacgag cagaagggct tcgggccgtg gctcgaaaag
    17341 cgcctgccgc agggcgagcg cagcgagttc gagcgcgagc tggagcgcta caagcgcgtc
    17401 gacaccgacg gcgtgccctt cgaggatgcc gcgctcagcg agcgcgagga ctcccgccag
    17461 ctcggcgccg acaacctgtc gctggcgcgg cgcttcgtga tccgcttcgc gcatctgggc
    17521 tggggcttcg gcccgtcgat cgacgcgctg ggcctcagga cctgcttcga ccacgaccac
    17581 tcgccggtgt cgatccaccg ctacgaccgg ctggcgatcg aggccttccc gcgcgcgaag
    17641 acctgcttct tccaggaggt caacatgccc gacgcgagct ggaacccgcg cggcctgccg
    17701 atcccgctga agcgcacgtt tgaaatcttc atcccgaacg tcagcggcat cgacatggat
    17761 accaacggca gctggccgta cggccggcgg ctggaggacc aggtcgcgac gcgcttcctg
    17821 tcgctgttcc tcgacatgaa gaccggctgc ggcggcaagc cgtgcaacgt cgagacgctg
    17881 agccagcagg cgctgtggga cggcgcgccg atcgagccga agacgccgcc gaatccgctg
    17941 cacaacgaca agcccttcct gaagcaattc ccgtatctcg ccgatccgtg gtgagggcgc
    18001 tcgtgcgtcg gtccggagcg gcttttgcgg cccggcactt tcccccgtga cgagggcggg
    18061 gccgggaaaa cgttgcagac cacgttttca agcccctttc cctccgggtg tcgtggtccg
    18121 gtttccatgg acacccgcac aggggaaatc acccccccga cgagggcgga gatcccatga
    18181 gtccccgccc tcggcgcagc ctggataccg tgggcaggtg gagaaagcag ggacaacaaa
    18241 tacagttcgt caggacaatg tccggacaac tacaatgatt gcatccagac tccggagcgg
    18301 cgccatgagc accctgaatc tttccaagac cgaacgcatc gacgttcggg caagcacgcc
    18361 agtcaagcaa ctgctgcagg aagccgcgcg cgcctgccac aagagcgtca gcgagttcct
    18421 gctcgacgcg ggcgtcactg cggccgcgca gacgctcgcg gatcgtcgcc agttcgtact
    18481 cgacgacgcg cagtggcagg cattccagga agcgctggat cgtcccgtgc aaagcaagcc
    18541 gcgcctgaag aagctgctgc gtgaacccgg gctgctcggt tgagccatgg ctacgcagcc
    18601 gttcgcaagc tggccgcaac ggatcaggtc gatgccttcg actgcggcca ggcggcgctg
    18661 aaccagttcc tgcagcgcta cgcgctcgtc aaccagaagg ccggcagcgc gcagacctac
    18721 gtttgctgcc gcgatgacgt ggtggtcggc ttctacagcc tggtggtcgg cagcgtcgat
    18781 ccggaggccg cgccgtcgag ggtgatgaaa gggctggcac gtcatccggt gccggtcatg
    18841 atcctggccc ggctcgcagt ggacacccag catcagcgca agggcctggg tcaggccctg
    18901 ctcagggacg ccctgctgcg aaccgcacag gccgccgaca tcgccggcat ccgttgcctg
    18961 ctgatccatg ccaaggatga tgcggcacga cgctggtacg aatcctggga attcgagccc
    19021 agcccgagtg atccgtatca cttgttcctg atgatcaagg atatcaatgg tttgctgagc
    19081 tgaaacagcg ttttcccgca aggctgcaca cccgggctgc ccgccccctt tcctgcaacg
    19141 gaagaagaac tgaacgacgg gggattcacc gtgatcggtt cgagccgtcc tgcacgcacc
    19201 accgccgtga acactccggg ccggattgcc cgtgtcgccg ccggtctgtg tgcgctcgtc
    19261 gtgatggcgg cagcggcgcg cgagcccgga tcgctcgcgc aggaactcgc gcgtctcgac
    19321 gccgcgctcg cgcgcgccga ag
//
LOCUS       DAOQIE010000025        19320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1280_length_19320_cov_7.347314, whole genome shotgun sequence.
ACCESSION   DAOQIE010000025 DAOQIE010000000
VERSION     DAOQIE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 19320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19320
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_19320_cov_7.347314"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..475)
                     /locus_tag="PK144_02975"
     CDS             complement(119..475)
                     /locus_tag="PK144_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64718.1"
                     /translation="MNQEQARTLIAALADGFDPDTGDALPADSVCQRPAVIRALYLAA
                     QALAAGGAMAAAPARELPRQAGKPRSQEEDEALAAAFKAGSSAGELAEAHCRTRGAIA
                     ARLVRLGLIEERKDLR"
     gene            complement(550..1938)
                     /gene="argH"
                     /locus_tag="PK144_02980"
     CDS             complement(550..1938)
                     /gene="argH"
                     /locus_tag="PK144_02980"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNF64719.1"
                     /translation="MSQTNQQWGGRFTESTDAFVQAFTASVQFDQRMYRQDIAGSIAH
                     ATMLAEAGVLNEADRAAIVAGLEAIRAEIERGEFEWSIALEDVHMNVEARLTERIGLA
                     GKRLHTGRSRNDQVATDIRLYLRDAIDAILAELARLQHGLVELAEREADTIMPGFTHL
                     QVAQPVSFGHHLLAWFEQLKRDSGRYRDCRARLNECPLGAAALAGTTYPIDRHRTASL
                     LGFAGPTENSLDSVSDRDFAIEFCSASALLMTHLSRFSEELVLWASAQFGFVDIPDRF
                     CTGSSIMPQKKNPDVPELVRGKSGRVHGHLVGLLTLMKGQPLAYNKDNQEDKEPLFDT
                     VDTVLGSLRAFADMVPALRARPERMRAAALQGFATATDLADYLVRKGVPFRDAHEIVG
                     KAVRLGVDTGRDLSQMRLEELRAFSDAIGADVFDSLTLEGSLAARNHFGGTAPAQVRA
                     AVQRARKWLAQD"
     gene            complement(2027..3106)
                     /locus_tag="PK144_02985"
     CDS             complement(2027..3106)
                     /locus_tag="PK144_02985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64720.1"
                     /translation="MSWSLSSAHTDFPAFAPAWDALNRRLFNAHPLLDSAFIAPLVEL
                     YGPDTLRLARETVDGQLRSAALIRPLAPGAWRVFALGPIQMTPALMAPEADWDALLRS
                     LPGMALLLNVNLQDPQYSHLAPCPPVRRAFDHACTIGIALEGDFDRYWQGRSRHLRQN
                     LGRYQRRLREDGMAVEWRQYAEPDAIVAAVDRYSVLESAGWKGRNGTALHPDNLQGEF
                     FRRVMQRFAHRASARVWELWIDGQLAASRLAIGTPDMLVMLKTTYDEQLARYAPGMLL
                     LRRVIEEEFTAARHRRIEFYTNATPAQHAWATDSRWIRHFEVYRNRLSASVIDGVRLS
                     RAHPRALLGGTAAVAALAAFSALLN"
     gene            complement(3222..3677)
                     /locus_tag="PK144_02990"
     CDS             complement(3222..3677)
                     /locus_tag="PK144_02990"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HNF64721.1"
                     /translation="MTVPRHPARQIALAFACSVVLLAACTGPTGSERATVTSDAYRHA
                     RPGELTGSVSWLQRLLPPPGAGLTVELIEAGPRARVLAEQRLARLGAPPWRFRLEFDP
                     ARIDPAQRYVLRARVDFDGRTRFANRLDYPVLTHDAPAHVDVIVDAALP"
     gene            complement(3693..4667)
                     /locus_tag="PK144_02995"
     CDS             complement(3693..4667)
                     /locus_tag="PK144_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNF64722.1"
                     /translation="MPPAAARTARGAPAFDRRWLGPRHWPSWGIAGLAWLLQCLPQRA
                     RDALARLVLPLVWRLTGRQQHNARINLGLCFPQQPPEWREQVLREHIALAVRVTLDYG
                     LLWFGSATAIERAVRFHGEEHLAAARAEGRRVILLVAHALALDFGICAFGIRHRALGP
                     YKALADPVIDWLIARARTRFEGSVFEREAGMRPVIRGFRDGQILVYLADEDLGPDGAV
                     FVPFFAEPKATLATLGRLAKLGDARVIPSVTRWNAAEGVYDCFHLPPLADFPSGDAER
                     DARAMNAAVEALITPEPAQYMWTLRLFRTRPSGRAKLYKQRKTPPAGG"
     gene            complement(4667..5566)
                     /locus_tag="PK144_03000"
     CDS             complement(4667..5566)
                     /locus_tag="PK144_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF64723.1"
                     /translation="MPRLISRTRRAALLEAVLRGLARLSLPAVHRLGDALGWLLAALP
                     NLPRRLAARNLELCLPELDAAARARLLRASLRETACGMLELGPLWCWPAERQRALVRA
                     SQGEAAFAAAVAAGHGVIVLSPHLGAWEVSGQYISSRWPITSLYRPSRLGIDTLVRSG
                     RERLGARLVPTDAGGVRALLAALRRGEVAGILPDQDPGRGNGVFAPFFGVSANTMTLV
                     SRLAQRSRAPVFLACAERLPRGAGFCVHFTPCSPAIGREPLAESVAALNADVEAAVRR
                     LPAQYLWAYKRFRTRPDGEAKLY"
     gene            complement(5586..6326)
                     /locus_tag="PK144_03005"
     CDS             complement(5586..6326)
                     /locus_tag="PK144_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNF64724.1"
                     /translation="MSSTHSGLRACALACTLLLGACAHQPQLAPLDPEAIDRLPAYNR
                     WMFGVNEKLDEYLLKPVAQGYQAVTPQFLDDGITNIFGNLADLPIGINNLLQFKFREA
                     AQDFTRIVFNSTFGLLGFFDVASYFGIPKHDEDFGQTLAHWGVGEGSYFVLPLLGPST
                     LRDTGGLAVDAFNIDPVWEINRIPVRNSLVGLRIVDKRADLLRSERVTEGALLDRYTQ
                     IRDAWLQRRRSLIYDGNAPLEPRRLDVD"
     gene            6483..6740
                     /locus_tag="PK144_03010"
     CDS             6483..6740
                     /locus_tag="PK144_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64725.1"
                     /translation="MPFALAPFLFVLRLPAVPPAAAGTPSAAGDARHPFCCALGAAAG
                     QAPRRSFRKDRIDRPGRLVASFPAQTGPQDPTLLTAIEKLL"
     gene            6755..7240
                     /locus_tag="PK144_03015"
     CDS             6755..7240
                     /locus_tag="PK144_03015"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HNF64726.1"
                     /translation="MTPSAGPSAGPSPSAQDPARASETAQWLCYVGLLPFIGATATLA
                     LGGEAWHDTALQALIGYGAVILGFLGAVHWGLALREQPLDHRRHYFGWGVLPALAGWL
                     ALMIPPRTGLGLLFAGYALQYAADRHATVQDALPLWYERLRLKLSQVVLLSLAIAWAL
                     A"
     gene            complement(7245..7703)
                     /locus_tag="PK144_03020"
     CDS             complement(7245..7703)
                     /locus_tag="PK144_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNF64727.1"
                     /translation="MQILTTLIALAGCLAIGALVLNGVRLGEPPGMGRRLLTYLNNHV
                     AETRRGHDFPELELRCYRMSPAQLYDRVEQALRVLNWEIVEASPQAQSLHATVTSQLL
                     RFVDDVHIELRVAACGTELHVRSQSRIGRGDFGANARHVMELHLTLDRLL"
     gene            complement(7822..8562)
                     /locus_tag="PK144_03025"
     CDS             complement(7822..8562)
                     /locus_tag="PK144_03025"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNF64728.1"
                     /translation="MRIPRIHVAGPIVAGEPVALDDSASNHVVRVLRLAPGAPLRLFD
                     GRGGQWRATLASCGKRGASALAEAHEALECESPLAVVLAQGIARGERMDYALQKAVEL
                     GVQAIRPVFTARSNVQLDADRLDKRLAHWRAVIVAACEQCGRNRLPVLAAPLRIEALL
                     AETAAGDGGLRLVLDPLAQHGLRALAPPAGPVTLLVGPEGGLAEAELAAATAAGWQGV
                     RLGPRVLRTETAGVAALAALAALWGDLG"
     gene            8640..9410
                     /gene="xth"
                     /locus_tag="PK144_03030"
     CDS             8640..9410
                     /gene="xth"
                     /locus_tag="PK144_03030"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNF64729.1"
                     /translation="MRKIATWNVNSLKVRLPQVLDWLAAESPDVLALQETKLQDADFP
                     AGAIRAAGYEVVFAGQKTYNGVALLSREPADAVVTELPGLADPQRRVLGATVAGVRVL
                     DLYVPNGQAVGSDKYAYKLDWLAKLAAFLETERAAHPELVVLGDFNIAPEDRDVHDPE
                     AWRGQILCSGPERAAFRGLLDAGFRDAFRLFEQPPKTFSWWDYRGGGFRRNHGLRIDH
                     ILLSPALAARCRGCRIDTGPRRLERPSDHAPVLASFDT"
     gene            9489..9914
                     /locus_tag="PK144_03035"
     CDS             9489..9914
                     /locus_tag="PK144_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64730.1"
                     /translation="MKIRILFLAAALLSGACGTPATAPSFRTSATVVLTTGLNDRQQP
                     VNAVEALSLDQERFHVFTQWNQLAPGEHTQLVRVLDGERKPVFSDAQRFTADNVSRST
                     VSSFQINRYLQQPGQWQVEVYLDNERFAQKGVKVLPAAN"
     gene            10067..11152
                     /locus_tag="PK144_03040"
     CDS             10067..11152
                     /locus_tag="PK144_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNF64731.1"
                     /translation="MERRDFLKTAGAGLAAAATVAAPAIVRAEPQIKWRLASSFPKSL
                     DTIYGAAEVLANRVREITEGKFDIRVFAGGEIVPGFQTLDAAQQGTVECGHATMYYFI
                     GKNKAFAFDTALPFGLTCRQQNAWLYHGGGLELYREFLKDYGVISFPGGNTGTQMGGW
                     FRNEIKGLADVKGLKMRIAGLGGEIMSRLGAVPQQIPGGDIYPSLEKGTIDAAEWIGP
                     YDDEKLGFVKVAPHYYFPGWWEGGPNVSFFVNQGEWDKLPKSYQAAFTAAAAEANIDM
                     MAKYDAKNPPALSRLVRDGAKLHPFPRDVMEAAFKTANELYEEEAGKNPQFKKLYDSW
                     KTFRSAEYQWFRVAEATYANFVYSQRL"
     gene            complement(11264..12727)
                     /locus_tag="PK144_03045"
     CDS             complement(11264..12727)
                     /locus_tag="PK144_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNF64732.1"
                     /translation="MEAFIVANMAPLMFGALVIFLLLGYPVAFALAANGMVFGLIGIE
                     YGLLKPELLQALPQRVFGIMQNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPV
                     RGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDKKLATGVIAASGTLA
                     QIIPPSLVLIIMADQLGRSVGDMYAGALIPGLVLTGMYVGYVFLTATFKPDAAPALPP
                     EARSLRGAALGRRVLVSLVPPLVLIFLVLGTIFIGVATPTEGGAMGASGAIILALINR
                     RLDWKLVRQALDQTAKLSCFVLFILIGSTVFGLVFRAVNGDLWVEHLLTALPGGEVGF
                     LIVVNILMFALAFFLDFFELAFILVPLLGPVAEKLGIDLIWFGVLLGVNMQTSFMHPP
                     FGFALFYLRSVAPPDIRTTHIYWGAVPFVCIQIIMVALLIAFPGLVSVEKHKVDNSAP
                     VQIEMPAVDYGAPAGDGGYAPPSYDYQPAPPPEPAKP"
     gene            complement(12743..13321)
                     /locus_tag="PK144_03050"
     CDS             complement(12743..13321)
                     /locus_tag="PK144_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNF64733.1"
                     /translation="MDFLLRLSRGIDWLNEHVGKTVYWLILIMVIICTGNALMRKLFN
                     ISSNGFLEIQWYLFAAVFLLGAAYTLQRNEHVRIDVVVGRFSLRTQIIIDILGTLLFL
                     TPLCLLMIVFGWPYFLDSWHTQEYSPNPGGLILWPVKVLIPIGFVLLQLQGLSQLIKQ
                     IGALTGHVDPHTLVKSHHGHAPADVPAPHTSN"
     gene            13587..15050
                     /gene="ubiD"
                     /locus_tag="PK144_03055"
     CDS             13587..15050
                     /gene="ubiD"
                     /locus_tag="PK144_03055"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HNF64734.1"
                     /translation="MKYKDLRDFIARLEADGQLRRIRVPVSPRLEMTEICDRVLRARG
                     PALLFERPAGHSVPVLANLFGTPERVALGMGAESVAALREVGRLLAFLKEPEPPKGLK
                     DAWNSLPVFRKVLDMAPKEISRPPCQECVLEGEAVDLARLPVQTCWPGDAGPLITWGL
                     VVTKGPHKARQNLGIYRQQVIGRNRVIMRWLSHRGGALDFREWQQAHPGRPFPVAVAL
                     GADPATILGAVTPVPDTLSEYAFAGLLRGAKTELARCLGCDLQVPASAEFVLEGHIAP
                     GDTAPEGPFGDHTGYYNEVDTFPVFTIERITHRREPIYHSTYTGRPPDEPAILGVALN
                     EVFVPILQKQFPEITDFYLPPEGCSYRLAVVTIRKQYPGHAKRVMLGVWSFLRQFMYT
                     KFVIVTDEDIDARRWEDVIWAMTTRMDPARDTVTIENTPIDYLDFASPVSGLGSKIGF
                     DATHKWPGETTREWGTPIRMDDAIRARVDSLWPELGL"
     gene            15193..17463
                     /locus_tag="PK144_03060"
     CDS             15193..17463
                     /locus_tag="PK144_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64735.1"
                     /translation="MSAQDFHGSTGAAGVPAADDDDLLFLPEGPEAGPERIASPQRWR
                     VLAVDDDRDFQRSTAFALSGMRVLGRRIELLQAYGHAEAARLLAGERDIAVMLLDVVM
                     ETDDAGLRLARAVREVLGNAELRIVLVTGQPGLAPVHEVMERYDINDYWTKSELGVER
                     LHTLLTAGVRAWEQLRTVARARRGLQMIVEASNVLVGARSLRDFSARTIAEIASVLGV
                     PPEGVVCVRRARDDGRVIGAAGRYARSVDRPLEALEPAGVREAIRHCLQERRTLVLAD
                     CMVLYFPAELAGAEYAAWVEIERSLDDTEQELLRVFALNIGSGLHNVALFSRLDDLAY
                     RDQLLGLPNRNALLRALESALGSAARHHTALLLIDIDNFSGVNLTLGPAYGDALLKRI
                     GERLRDGFDPTVLLARLGGDVFGALGPVERLDPESVAALFADGAPSAADGGAWSVGTA
                     RLPLAEFTGTALEALASATLLLKQAKLRGHGQHQDWLPEIAVAAGRRFALLQALREAL
                     ARDEIGIELQPQIDLATGTVTGVEALARWQRADGTRVAPDAFIPLAEATGLIVPLGEH
                     FIELACAAAARLAAAGHGHIRVAVNVSAVQSARADLVASIERQLLASGAEARQMEIEL
                     TETVAMQNFGAVTDTLERLRARGLTVAIDDFGTGFSSLAYLRRLPADLIKIDRSFVQE
                     LGNGGESRTEIAELIIRLAERLGLRVLAEGVETEAQAGWLRMHGCHYAQGWLYARAMP
                     LDALLDWLARREHAGT"
     gene            complement(17658..17969)
                     /locus_tag="PK144_03065"
     CDS             complement(17658..17969)
                     /locus_tag="PK144_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64736.1"
                     /translation="MLTEQECLDLCELSEEEIQAIAEHEHVPEVIAAELGQCLVQTHA
                     GQWLIRRYIMEDIAHAQVMGRVGRVAELNGVLERFEANHPTYDLRPKPPRKLPQPYRP
                     H"
     gene            18307..18382
                     /locus_tag="PK144_03070"
     tRNA            18307..18382
                     /locus_tag="PK144_03070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18340..18342,aa:Lys,seq:ctt)
     gene            complement(18479..19285)
                     /locus_tag="PK144_03075"
     CDS             complement(18479..19285)
                     /locus_tag="PK144_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF64737.1"
                     /translation="MKQDSAPQVPAVLVVDDEPEALQELLEFLDLNGYRCHGATGVDD
                     ALATFAADAGIGIVITDLNLAIGNGNELVGRLGLDFGDTRAFEAILVSGYAGKEDVIE
                     ALRRGAIDFLNKPLSGDELLDAVRRAAVRLARRSPGGERLEARLDEMMDTVLRLGEEV
                     SGLRRRLEAAPPEAAPATTAAALSDSEPLPELTPRMWEVLAHVGAGKTNYVIACDLGL
                     TENTVKLYVSQILGKTRVSNRTQLALRAAAWLAAAEARGLLPVRPEPPAG"
BASE COUNT         2898 a         6604 c         6958 g         2860 t
ORIGIN      
        1 tgggcgcaca cggcccgatc cggctgcaca tcgtgctgat cggcgactga gcggggagct
       61 gccgtgaacg acgcgggcgc cgtccgggag ggcggcgccc gcgtcgcatt tccggacttc
      121 agcgtaaatc cttgcgttct tcgatcagcc cgagcctcac cagccgggcg gcgatggcgc
      181 cccgggtgcg acaatgggct tcggcgagtt cgccggcgct gctgccagcc ttgaacgcgg
      241 ccgccagcgc ctcgtcctct tcttgcgacc gcggcttgcc ggcctgacgc ggcagctcgc
      301 gggccggtgc agccgccatg gcgcccccgg ctgccagcgc ctgcgctgcc agatacagcg
      361 cgcggatcac ggcggggcgc tggcagacgc tgtcggctgg cagcgcatcg ccggtgtccg
      421 gatcgaaacc gtccgcgagt gctgcgatca gcgtgcgggc ctgttcctgg ttcatgtccg
      481 ggtttcctcc ctgaatgagg atgaaaacgg ccgggatgtc cggaccggcg accggtcggc
      541 aggcccggat cagtcttgcg ccagccactt ccttgcccgc tgcaccgccg cccgcacctg
      601 cgccggcgcc gtgccgccga agtgattgcg ggcggcgagc gagccttcga gggtcaggct
      661 gtcgaacacg tcggcgccga tcgcgtcgct gaaggcacgc agttcttcga ggcgcatctg
      721 cgagaggtcg cggccggtgt cgacgccgag gcgcacggcc ttgccgacga tctcgtgcgc
      781 gtcgcgaaac ggcacgccct tgcgcacgag gtaatcggcg aggtcggtgg cggtcgcgaa
      841 gccctgcagc gcggcggcgc gcatgcgctc ggggcgggcc ctgagtgccg gcaccatgtc
      901 ggcgaaggcg cgcagcgagc cgagcacggt gtcgacggtg tcgaacagcg gctccttgtc
      961 ctcctggttg tccttgttgt acgcgagcgg ctgacccttc atcagcgtca gcaggccgac
     1021 cagatggcca tgcacgcggc cgctcttgcc gcgcacgagt tccggcacgt cggggttctt
     1081 cttctgcggc atgatcgagc tgccggtgca gaagcgatcc ggaatgtcga cgaagccgaa
     1141 ctgcgcgctc gcccacagca ccagttcctc gctgaagcgc gacaggtggg tcatcagcag
     1201 cgccgaggcc gagcagaatt cgatcgcgaa gtcgcggtcg ctgaccgaat cgagcgagtt
     1261 ctcggtcggt ccggcgaagc cgagcaggct ggcggtgcgg tggcggtcga tcggataggt
     1321 ggtgccggcg agtgccgcgg cgccgagcgg gcattcgttc aggcgtgcgc ggcagtcgcg
     1381 gtaacggccg ctgtcgcgct tgagctgttc gaaccacgca agcaggtggt ggccgaagct
     1441 gaccggctgc gcgacctgca ggtgcgtgaa gccgggcatg atggtgtcgg cctcgcgctc
     1501 ggcgagttcg accagcccgt gctgcaggcg cgcgagttcg gcgaggatcg cgtcgatggc
     1561 gtcgcgcagg tacaggcgga tgtcggtggc gacctgatcg ttgcgcgagc ggccggtgtg
     1621 caggcgcttg ccggcgaggc cgatgcgctc ggtcaggcgc gcctcgacgt tcatgtgcac
     1681 atcttcgagc gcgatcgacc attcgaactc gccgcgctcg atctcggcgc ggatggcttc
     1741 cagtccggcg acgatcgccg cgcggtcggc ctcgttcagc acgccggcct cggcgagcat
     1801 ggtcgcgtgc gcgatcgagc cggcgatgtc ctggcggtac atgcgctggt cgaactgcac
     1861 cgaggcggtg aaggcctgca cgaaggcgtc ggtggattcg gtgaagcgtc cgccccactg
     1921 ctgattggtc tggctcatgg ctgcatcggt ccgccgccgc aggaaaaagg gcggcaagta
     1981 tacggcgcga tccctgctgc ggaccgcgcg ccgatcgcgc cggggttcag ttgagcagtg
     2041 ccgaaaacgc cgccagcgcg gccacggccg ccgtgccgcc gagcagcgcg cgcggatggg
     2101 cgcggctcag gcgcacgccg tcgatgacgc tcgccgacag gcggttgcgg tagacctcga
     2161 agtgacggat ccagcggctg tcggtcgccc aggcgtgctg cgccggcgtg gcgttggtat
     2221 agaactcgat gcgccggtgc cgtgcggccg tgaattcctc ctcgatgacc cggcgcagca
     2281 gcagcatgcc cggtgcgtag cgggcgagtt gctcgtcata cgtggtcttc agcatgacca
     2341 gcatgtcggg cgtgccgatc gccagccggg aagcggcgag ctgcccgtcg atccagagct
     2401 cccagaccct ggcgctggca cggtgcgcga agcgctgcat cacgcgccgg aagaactcgc
     2461 cctgcaggtt gtccggatgc agcgccgtgc cgttgcggcc tttccagccg gcgctttcca
     2521 gcacgctgta gcgatcgacc gccgcgacga tcgcgtccgg ctcggcgtac tggcgccatt
     2581 cgacggccat gccgtcctcg cgcaaccggc gctggtagcg gccgaggttc tggcgcaggt
     2641 ggcgggagcg gccctgccag tagcggtcga aatcgccctc cagtgcgatg ccgatggtgc
     2701 aggcgtgatc gaaggcgcgg cgcaccggcg ggcagggggc gagatgcgag tactgcggat
     2761 cctgcaggtt gacgttcagc agcagcgcca tgccggggag gctgcgcagc agggcgtccc
     2821 agtcggcctc gggggccatc agcgccggag tcatctggat cgggccgagc gcgaacacgc
     2881 gccaggcacc gggggccagc ggccggatca gcgcggccga gcgcagctgg ccgtcgacgg
     2941 tctcgcgggc gaggcgcagc gtgtccggac cgtacagttc gaccagtggc gcgatgaatg
     3001 cgctgtcgag cagcgggtgc gcgttgaaca gccgtcggtt cagggcatcc catgccggtg
     3061 cgaaggcagg gaaatccgta tgggcggagg aaagcgacca ggacatgtcc aatctcggta
     3121 ttgcgcgctt cctgcgccgg ctatcggggt cgggacacag tccttcgctg tgtcaggccc
     3181 ggcagcatag gcaaaaccac gttcagtaca agtgattccg gtcatggcag cgctgcatcg
     3241 acgatgacgt cgacgtgcgc cggggcatca tgagtcagca ccgggtagtc gagacggttg
     3301 gcaaagcggg tgcggccgtc gaagtcgacg cgggcgcgca gcacgtaacg ctgcgccgga
     3361 tcgatgcgtg ccgggtcgaa ttcgagccgg aagcgccacg gcggtgcgcc gagtcgtgcc
     3421 agccgctgct cggccagcac ccgggcccgg gggccggcct cgatcagttc gaccgtcagt
     3481 ccggcgccgg gcggcggcag caggcgctgc agccagctga cgctgccggt cagttcgccg
     3541 gggcgggcgt ggcggtaggc atcgctggtc acggtcgccc gttcgctgcc ggtcgggccg
     3601 gtgcaggcgg cgagcagcac gacgctgcag gcgaaggcga gggcgatctg gcgggccgga
     3661 tggcggggca cggtcatgat ggggcagggt cctcagccgc cggcgggcgg ggtcttgcgc
     3721 tgcttgtaga gcttggcgcg gccgctcggg cgcgtgcgga acaggcgcag cgtccacatg
     3781 tactgcgccg gctcgggggt gatcagcgcc tcgacggcgg cgttcatcgc gcgcgcgtcg
     3841 cgctcggcgt caccactcgg gaaatccgcg agcggcggca ggtggaagca gtcgtagacg
     3901 ccttcggcgg cgttccagcg cgtcaccgac gggatcacgc gcgcgtcccc gagcttcgcg
     3961 agccgcccga gcgtcgcgag cgtcgccttc ggctcggcga agaacggcac gaacaccgca
     4021 ccgtcggggc cgaggtcctc gtcggccaga tacacgagga tctgcccgtc ccgaaagccg
     4081 cggatcaccg ggcgcatgcc ggcctcgcgt tcgaacaccg aaccttcgaa gcgcgtgcgg
     4141 gcgcgcgcga tcagccagtc gatgaccgga tcggccagcg ccttgtacgg cccgagcgcg
     4201 cggtggcgga tgccgaaggc gcagatgccg aaatcgagcg ccagcgcgtg cgcgaccagc
     4261 aggatcacgc gccggccttc ggcgcgggcc gcagcgaggt gctcctcgcc gtggaagcgc
     4321 acggcgcgct cgatcgccgt ggcgctgccg aaccacagca ggccgtagtc gagcgtcacg
     4381 cgcacggcca gcgcgatgtg ctcgcgcagc acctgctcgc gccattcggg cggctgctgc
     4441 ggaaagcaca ggccgagatt gatgcgggcg ttgtgctgct gcctgccggt cagccgccag
     4501 accagcggca gcacgagccg cgccagggcg tcacgcgcgc gctgcggcag gcattgcagc
     4561 agccaggcga gaccggcgat gccccaggac ggccagtggc gcggtccgag ccagcgccgg
     4621 tcgaaggccg gagcaccccg ggcagtgcgg gcggcagcgg gcggcatcag tacagcttcg
     4681 cctcgccgtc cggacgggtc cggaagcgct tgtaggccca cagatactgc gccggcagcc
     4741 ggcgcacggc ggcctccacg tcggcgttga gcgcggcgac cgactccgcg agcggttccc
     4801 ggccgatcgc cggcgagcac ggcgtgaaat gcacgcagaa gccggcgccg cgcggcagcc
     4861 gttcggcgca ggcgaggaat accggcgcgc ggctgcgctg ggcgaggcgc gagaccagcg
     4921 tcatcgtgtt ggcgctgacg ccgaagaacg gtgcgaacac gccgttgccg cggccggggt
     4981 cctgatccgg caggatgccg gcgacctcgc cgcgcctgag cgccgcgagc agcgcccgca
     5041 cgccgccggc atcggtcggc accagccgcg cgccgagccg ctcgcggccg ctgcgcacca
     5101 gcgtgtcgat gccgaggcgc gagggccggt acaggctggt gatcggccag cgcgaggaga
     5161 tgtactggcc gctgacctcc caggcgccga ggtgcggcga cagcacgatg accccgtgcc
     5221 cggccgccac cgctgccgcg aacgccgcct cgccctggct cgcgcgtacc agcgcgcgct
     5281 ggcgttcggc cggccagcac cacagcggtc cgagttcgag catgccgcag gcggtctcgc
     5341 gcaggctggc gcgcagcagc cgcgcgcggg ccgcagcgtc gagttcgggc aggcagagtt
     5401 cgaggttgcg cgccgcgagc cggcgcggca ggttcggcag cgctgcgagc agccagccca
     5461 gcgcatcgcc gagccggtgc acggccggca gcgacagccg cgcgaggccg cgcagcacgg
     5521 cttcgagcag cgccgcacgg cgggtgcggg agatcaggcg gggcatcgcg cgctgcagcg
     5581 ctcgctcagt cgacgtcgag gcgccgcggc tccagcggag cgttgccgtc gtagatcagg
     5641 ctgcggcggc gctgcagcca ggcatcgcgg atctgcgtgt agcgatcgag cagcgcgcct
     5701 tcggtgacgc gctcgctgcg cagcaggtcg gcacgcttgt cgacgatgcg cagccccacc
     5761 agcgagttgc gcaccgggat gcggttgatc tcccagaccg gatcgatgtt gaacgcatcg
     5821 accgccaggc cgccggtgtc gcgcagcgtg ctcgggccga gcagcggcag cacgaagtag
     5881 ctgccctcgc cgacgcccca gtgcgcgagc gtctggccga agtcctcgtc gtgcttcggg
     5941 atgccgaagt agctcgccac gtcgaagaag ccgagcaggc cgaaggtcga gttgaagacg
     6001 atgcgcgtga agtcctgcgc ggcttcccgg aacttgaact gcagcaggtt gttgatgccg
     6061 atcggcaggt cggcgaggtt gccgaagatg ttggtgatgc cgtcgtcgag gaactgcggt
     6121 gtcaccgcct gatagccctg cgcgaccggc ttgagcagat attcgtccag cttctcgttg
     6181 acgccgaaca tccagcggtt gtacgccggc agccggtcga tcgcttcggg atcgagcggc
     6241 gcaagctggg gctggtgggc gcaggcgccg agcagcagcg tgcaggcgag cgcgcaggcg
     6301 cgcaggccgg agtgggtgga tgacatgacc ccggatcctc gggctgaaga caggaacgat
     6361 gcggccgatg atacctgcat gccgcctcgc ccggccatgc agcgatcggc aggcaggcga
     6421 accgccggcg acggacgcgt tccaaatcag acctgcccgt cctgacgttc cggggagcca
     6481 ccatgccgtt cgcgctcgcg ccgttcctgt tcgtcctgag gctgccggcc gtgccgccgg
     6541 cggctgccgg gaccccgtcc gcggccggtg acgcccggca tccgttctgc tgcgcgctcg
     6601 gcgcggctgc cggccaggcg ccgcgccgga gcttccggaa ggaccggatc gaccgtccgg
     6661 gacggcttgt tgcaagtttt cctgcgcaga ccggcccgca agaccccaca ctgctcacag
     6721 cgatcgaaaa gctgctctga aggagcccgc gatcatgact ccatctgccg gtccatccgc
     6781 cggtccatcc cccagcgcgc aggatccggc ccgcgcgtcc gaaacggcgc agtggctctg
     6841 ctatgtcggc ctgctgccct tcatcggcgc aaccgccacg ctcgccctcg gcggcgaggc
     6901 ctggcacgac accgcgctgc aggcgctgat cggctacggc gcggtgatcc tcggtttcct
     6961 cggggccgtg cactgggggc tggcgctgcg cgagcagccg ctcgatcacc gccggcacta
     7021 cttcggctgg ggcgtgctgc cggcgctggc cggctggctg gcgctgatga tcccgccgcg
     7081 caccggcctc ggcctgctgt tcgccggcta cgcgctgcag tacgccgccg atcgtcacgc
     7141 gaccgtgcag gacgcgctgc cgctctggta cgagcggctg cggctgaagc tgtcgcaggt
     7201 cgtgctgctg tcgctggcga tcgcctgggc gctggcctga cgcgctacag cagccggtcc
     7261 agcgtcaggt gcagttccat gacgtggcgg gcgttggcgc cgaagtcgcc gcggccgatg
     7321 cgcgattgcg agcgcacgtg cagctcggtg ccgcaggcgg cgacgcgcag ttcgatgtgc
     7381 acgtcatcga cgaagcgcag cagctggctg gtcacggtcg cgtgcagtga ctgcgcctgc
     7441 gggctggcct cgacgatctc ccagttcagg acccgcagcg cctgttcgac gcggtcgtac
     7501 agctgcgccg gcgacatccg gtagcagcgc agttccagct ccgggaaatc gtggccgcgc
     7561 cgggtctcgg cgacatggtt gttcaggtag gtcagcaggc gccggcccat gccgggcggt
     7621 tcgccgaggc gcaccccgtt cagcaccagc gccccgatgg ccagacagcc ggccagcgcg
     7681 atcagcgtcg tcaggatctg catcgttctt cgcatcctca tgtggttcga gcccgtgtgg
     7741 cgggaggccg cggttgtggc gagcagcccg ggggacggag tctgcagcgc tgcgcggggt
     7801 tcccggtgct ggcgatcgaa cctagcccag atcgccccac agcgctgcca gtgcggccag
     7861 tgccgcgacg ccggcagtct cggtgcgcag cacgcgcgga ccgagccgca cgccctgcca
     7921 gccggccgcc gtggccgccg ccagctccgc ttcggccagc ccgccttcgg gcccgaccag
     7981 cagcgtcacc ggtcccgcag gcggcgcgag cgcacgcagg ccgtgctgtg ccagcgggtc
     8041 gagcaccagc cgcaggccgc cgtcaccggc ggcggtttcg gcgagcagcg cctcgatgcg
     8101 cagcggcgcg gccagcaccg gcaggcggtt gcggccgcac tgctcgcagg cggcgacgat
     8161 gacggcgcgc cagtgtgcga ggcgcttgtc gaggcgatcg gcgtcgagct gcacgttcga
     8221 gcgcgccgtg aacaccggcc ggatcgcctg cacgccgagt tcgacggcct tctgcagcgc
     8281 gtagtccatg cgctcgccgc gggcgatgcc ctgcgcgagc acgacggcga gcggcgattc
     8341 gcattcgagc gcttcgtgcg cttcggcgag cgcgctggcg ccgcgcttgc cgcagctcgc
     8401 cagcgtcgcc cgccactggc cgccgcggcc gtcgaacagc cgcagcggcg cgcccggcgc
     8461 gaggcgcagc acgcgcacga cgtggttgct ggcgctgtca tcgagcgcga ccggctcgcc
     8521 ggcgacgatc ggaccggcga cgtggatgcg gggaattctc atgcgggcgg catccggacg
     8581 gacggcgggg gaagggcgcc attgtatgct gcgcccccgt ccccgaccgg aagccgccga
     8641 tgcgcaagat cgccacctgg aacgtcaact cgctgaaggt gcggctgccg caggtgctcg
     8701 actggctcgc cgccgaaagc ccggacgtgc tggcgctgca ggaaaccaag ctgcaggacg
     8761 ccgatttccc ggccggggcg atccgcgccg ccggctacga ggtcgtcttc gccggccaga
     8821 agacctacaa cggcgtcgcg ctgctgtcgc gcgagccggc agacgcggtg gtgaccgagc
     8881 tgcccgggct cgcggacccg cagcggcgcg tgctcggggc cacggtcgcc ggcgtgcgcg
     8941 tgctcgatct gtacgtgccg aacggtcagg cggtcggcag cgacaagtac gcctacaagc
     9001 tcgactggct ggcgaagctc gcggcctttc tcgaaaccga gcgtgccgcg catcccgaac
     9061 tcgtcgtgct cggggacttc aacatcgcgc ccgaggatcg cgacgtgcat gaccccgaag
     9121 cctggcgcgg acagatcctg tgctccgggc ccgagcgtgc cgccttccgc ggcctgcttg
     9181 atgcgggctt ccgcgatgcg ttccggctgt tcgagcagcc gccgaagacg ttctcgtggt
     9241 gggattaccg cggcggcggc ttccgccgca atcacggtct gcgcatcgat cacatcctgc
     9301 tgtcgccggc gctcgcggcg cgctgccgtg gctgccggat cgacaccggg ccgcggcggc
     9361 tggagcggcc gtccgaccac gcgccggtgc tggcgagctt cgacacctga gccggccggc
     9421 tgcggacgct gcggtatagt gccggcccgg ccgtcaccgc cccgcccgcc ccatcgagtt
     9481 tcgccgtcat gaaaatccgc atcctgttcc tggccgctgc gctcctgtcg ggcgcctgcg
     9541 gcactccggc caccgccccg tccttccgca ccagcgcgac cgtcgtgctg accaccggcc
     9601 tcaacgatcg ccagcagccg gtcaacgccg tcgaggccct gagcctcgat caggagcgct
     9661 tccacgtgtt cacgcagtgg aaccagctcg ccccgggcga gcacacgcag ctggtgcgcg
     9721 tgctcgacgg cgagcgcaag ccggtgttct cggatgccca gcgcttcacg gccgacaacg
     9781 tcagccgctc gaccgtcagc agcttccaga tcaaccgcta cctgcagcag cccgggcagt
     9841 ggcaggtcga ggtctacctc gacaacgagc gcttcgcgca gaagggagtc aaggtcttgc
     9901 cggccgccaa ctgagttggt cttattgtcg gaccagtcgg gcgacggggc tgcgtgagca
     9961 gccccgtttc gtttccggaa tccgtcaggc ttggcgctgg cagtccggat gcactgccta
    10021 tactggcccg ggccatgagc ccccctgata aagcgaggag aagaaaatgg agagacgcga
    10081 cttcctgaag accgccggtg ccggtctggc cgccgccgcg accgttgccg ccccggcgat
    10141 cgtgcgcgcc gaaccgcaga tcaaatggcg actggcatcg agcttcccga agagcctcga
    10201 cacgatctac ggcgccgccg aggtgctcgc caaccgcgtg cgcgagatca ccgaaggcaa
    10261 gttcgacatc cgcgtgttcg ccggcggcga gatcgtcccc ggcttccaga cgctcgacgc
    10321 cgcccagcag ggcacggtcg agtgcggcca cgcgacgatg tactacttca tcggcaagaa
    10381 caaagccttc gccttcgaca ccgcgctgcc gttcggcctg acctgccgcc agcagaacgc
    10441 ctggctgtat cacggcggcg gtctggagct gtaccgcgag ttcctgaagg actacggcgt
    10501 catcagcttt ccgggcggca acaccggcac gcagatgggc ggatggttcc gcaacgaaat
    10561 caagggactg gccgacgtca agggcctgaa gatgcgcatt gccggcctcg gcggcgagat
    10621 catgtcgcgc ctcggcgccg tgccgcagca gattccgggt ggcgacatct acccgtcgct
    10681 ggaaaagggc acgatcgatg cggccgaatg gatcgggccg tacgacgacg agaagctcgg
    10741 cttcgtgaag gtcgcgccgc attattactt ccccggctgg tgggaaggcg ggccgaacgt
    10801 cagcttcttc gtcaaccagg gcgaatggga caagctgccg aagagctacc aggcggcgtt
    10861 cacggcggcg gcggccgagg ccaacatcga catgatggcc aagtacgacg ccaagaaccc
    10921 gccggcgctg agccggctgg tgcgcgacgg cgccaagctg cacccgttcc cgcgcgatgt
    10981 catggaagcg gcgttcaaga cggccaacga gctgtatgag gaagaggccg gcaagaaccc
    11041 gcagttcaag aagctctacg actcctggaa gaccttccgt tcggccgagt accagtggtt
    11101 ccgcgtcgcc gaggcgacct acgcgaactt cgtctacagc cagcgcctgt aagtcgcagc
    11161 acaggcagca aaaagccccg ccatccggcg gggctttttc ttgggtcttc gcggctgttt
    11221 tccccgctgc cgcccggatc ggcggcagcg gggagcgggc cgctcagggt ttggccggct
    11281 cgggcggcgg ggccggctga tagtcgtagc tcggcggcgc gtagccaccg tcgccggccg
    11341 gggcgccgta gtcgacggcc ggcatctcga tctgcaccgg ggcgctgttg tcgaccttgt
    11401 gcttctcgac gctgaccaga cccgggaagg cgatcagcag cgccaccatg atgatctgga
    11461 tgcacacgaa cggcaccgca ccccagtaga tgtgcgtggt gcggatgtcg ggcggcgcga
    11521 ccgagcgcag gtagaacagc gcgaagccga acggcgggtg catgaagctc gtctgcatgt
    11581 tgacgccgag cagcacgccg aaccagatca ggtcgatgcc gagcttctcg gcgaccgggc
    11641 cgagcagcgg cacgaggatg aaggccagct cgaagaagtc gaggaagaag gccagcgcga
    11701 acatcaggat gttgacgacg atcaggaagc cgacctcgcc gcccggcagc gccgtcagca
    11761 ggtgctcgac ccacagatcg ccgttgacgg cgcggaacac cagaccgaac accgtcgagc
    11821 cgatcaggat gaacagcacg aagcacgaca gcttggcggt ctggtcgagc gcctgacgca
    11881 cgagcttcca gtcgagacgg cggttgatca gcgccaggat gatcgcgccc gaggcaccca
    11941 tcgcgccgcc ttcggtcggt gtcgcgacgc cgatgaagat cgtgccgagc acgaggaaga
    12001 tcagcaccag cggcggcacc agcgacacca gcacgcgccg cccgagcgct gcgccgcgca
    12061 ggctgcgggc ctcgggaggc agcgctggtg ccgcgtccgg cttgaaggtc gcggtcagga
    12121 acacgtagcc gacgtacatg ccggtcagca ccaggcccgg aatcagcgca ccggcataca
    12181 tgtcaccgac cgaacggccg agctggtcgg ccatgatgat cagcaccagc gacggcggga
    12241 tgatctgtgc gagcgtgccg gaagcggcga tgacgccggt cgccagcttc ttgtcgtagc
    12301 cgtagcgcag catgatcggc agcgagatca ggcccatcga gatcaccgag gccgcgacca
    12361 cgccggtggt cgccgcgagc agcgcaccga cgaagatcac cgcgtaggcg agaccgccgc
    12421 gtaccggtcc gaacagctgg ccgatggtgt cgagcaggtc ctcggccatg cccgagcgtt
    12481 cgaggatcag gcccatgaac gtgaagaacg ggatcgcgag cagcgtgtcg ttctgcatga
    12541 tgccgaacac gcgctgcggc agcgcctgca gcagctcggg cttcagcagt ccgtattcga
    12601 tgccgatcag gccgaagacc atgccgttgg ccgccagcgc gaacgcgacc ggatagccga
    12661 gcagcaggaa gatcacgagg gcgccgaaca tcagcggcgc catgttggca acaatgaagg
    12721 cttccatcgg gggcgcgctc cgtcagttcg aggtgtgcgg cgcgggcacg tcggcgggcg
    12781 cgtggccgtg gtgcgacttg accagcgtgt gcggatcgac gtgtccggtc agcgcgccga
    12841 tctgcttgat cagctgcgac aggccctgca gctgcagcag cacgaaaccg atcgggatca
    12901 ggaccttcac cggccagagg atcaggccgc cggggttcgg cgagtattcc tgcgtgtgcc
    12961 aggaatcgag gaagtacggc cagccgaaca cgatcatcag caggcacagc ggcgtcagga
    13021 agagcagcgt gccgaggatg tcgatgatga tctgcgtgcg caggctgaag cgcccgacga
    13081 ccacgtcgat gcgcacgtgc tcgttgcgct gcagcgtgta ggcggcaccg agcaggaaca
    13141 ccgccgcgaa caggtaccac tggatttcga ggaagccgtt cgagctgatg ttgaacagct
    13201 tgcgcatcag cgcgttgccc gtgcagatga tcaccatgat caggatcagc cagtacacgg
    13261 tcttgccgac gtgctcattg agccagtcga tgccacggga aagtctcagc aggaagtcca
    13321 ccctttgttt ctcctccggt cgtgcgccgg cttgccggcg cgcgtggggg gcgggccggg
    13381 ggtgccgtgc gggcttttat ggggatgggc tccgggtcgc ggagtccgcg cagcttacac
    13441 tgttctggtc aaatggtcat accggcgctt tcgcggatca cgctgccggc gaccggccta
    13501 gtgtagagtc ggctccgcgc ggctgccggc acgcccgccg ccgcttcccc ccgtcgtccg
    13561 gatgaccgtc aggtcgttga gtcccgatga aatacaaaga cctgcgtgac ttcattgccc
    13621 gcctcgaagc cgatggccag ctgcggcgca tccgcgtgcc ggtgtcgccg cggctggaga
    13681 tgaccgagat ctgcgatcgc gtgctgcgcg cgcgcgggcc ggcgctgctg ttcgagcgtc
    13741 cggccgggca ctcggtgccg gtgctggcga acctgttcgg cacaccggag cgcgtcgcgc
    13801 tcggcatggg cgcggagtcg gtcgcggcgc tgcgcgaggt cggcaggctg ctggccttcc
    13861 tgaaggagcc ggagccgccg aagggcctga aggacgcctg gaacagcctg ccggtgttcc
    13921 gcaaggtgct cgacatggca ccgaaggaga tctcgcgccc gccgtgccag gaatgcgtgc
    13981 tcgaaggcga ggcggtggat ctcgcgcgcc tgccggtaca gacctgctgg cccggcgatg
    14041 ccggaccgct gatcacctgg gggctggtgg tcacgaaggg gccacacaag gcccggcaga
    14101 atctcggcat ctaccgccag caggtgatcg gccgcaaccg cgtgatcatg cgctggctgt
    14161 cgcaccgcgg cggtgcgctc gacttccgcg aatggcagca ggcgcacccg ggccggccgt
    14221 ttccggtggc ggtggcgctc ggggccgatc cggccaccat cctcggggcc gtgacgccgg
    14281 tgccggatac cctgtccgaa tacgcgttcg ccggcctgct gcgcggggcg aagaccgaac
    14341 tcgcgcgctg cctcggctgc gacctgcagg tgccggccag cgccgagttc gtgctcgaag
    14401 gccacatcgc gcccggcgac acggcgcccg aaggaccgtt cggcgatcac accggctact
    14461 acaacgaggt cgacaccttt ccggtgttca cgatcgagcg catcacgcac cggcgcgagc
    14521 cgatctatca ctcgacctac accggccgcc cgcccgacga gccggcgatc ctcggcgtgg
    14581 cgctgaacga ggtgttcgtg ccgatcctgc agaagcagtt tccggagatc accgacttct
    14641 atctgccgcc cgagggctgc tcgtaccggc tggcggtcgt gaccatccgc aagcagtacc
    14701 cgggccacgc caagcgcgtg atgctcggcg tctggagctt cctgcgccag ttcatgtaca
    14761 cgaagttcgt gatcgtcacc gacgaggaca tcgatgcccg ccgctgggag gacgtgatct
    14821 gggcgatgac cacgcgcatg gacccggcgc gcgacacggt gacgatcgaa aacacgccga
    14881 tcgactatct ggatttcgcg tcgccggtct cggggctcgg ctcgaagatc ggcttcgatg
    14941 ccacccacaa gtggcccggc gaaaccacgc gcgagtgggg caccccgatc cgcatggacg
    15001 acgcgatccg cgcgcgcgtc gacagcctgt ggcccgagct ggggctgtga gccgcatgag
    15061 gcgcaaagct tgtggtgcgg tgcaacatga atggccggca aacgtttatg ctgcgccctc
    15121 gtccgggtcc gtgacccggc acacgtcagg gaagaccggc gcatgcgggc ggccggtcag
    15181 cggagacgga cgatgagcgc gcaagacttc catggcagca ccggagcggc cggcgtcccg
    15241 gcagccgatg acgacgatct gctgttcctg cccgaagggc cggaggccgg gccggagcgg
    15301 attgcgagcc cgcagcgctg gcgcgtgctc gccgtcgacg atgaccgcga cttccagcgc
    15361 tcgaccgcct tcgcgctgtc cggcatgcgc gtgctcggcc ggcgcatcga gctgctgcag
    15421 gcctacggcc acgccgaggc cgcgcgcctg ctcgccggcg agcgcgacat cgcggtgatg
    15481 ctgctcgacg tggtcatgga aaccgacgac gccggcctgc ggctggcgcg cgcggtgcgc
    15541 gaggtgctcg gcaacgcgga gctgcgcatc gtgctcgtga ccgggcagcc ggggcttgcg
    15601 ccggtgcacg aagtcatgga gcgctacgac atcaatgact actggaccaa gtccgaactc
    15661 ggcgtcgaac gcctgcacac gctgctgacc gccggtgtgc gcgcctggga gcagctgcgc
    15721 accgtggcgc gcgcccggcg cggcctgcag atgatcgtcg aggccagcaa cgtgctggtc
    15781 ggcgcgcgca gcctgcgcga cttctcggcg cgcacgatcg ccgagatcgc ctcggtgctc
    15841 ggcgtgccgc ccgaaggcgt ggtctgcgtg cgccgcgcgc gcgacgacgg ccgcgtgatc
    15901 ggggccgccg gccgctacgc gcgcagcgtc gaccgtccgc tcgaagccct ggagccggcc
    15961 ggcgtgcgcg aggcgatccg ccactgcctg caggagcggc gcacgctggt gctcgccgac
    16021 tgcatggtgc tgtacttccc ggccgagctg gccggggccg aatacgcggc ctgggtcgag
    16081 atcgagcgct cgctcgatga caccgaacag gaactgctgc gcgtgttcgc gctgaacatc
    16141 ggcagcggcc tgcacaacgt cgcgctgttc agccgcctcg acgatctcgc ctaccgcgac
    16201 cagctgctcg gcctgccgaa ccgcaacgcg ctgctgcgcg cgctggaaag cgcgctcggc
    16261 agcgccgccc gccaccacac cgcgctgctg ctgatcgaca tcgacaactt ctccggcgtc
    16321 aacctgacgc tcggcccggc ctacggcgac gccctgctca agcgcatcgg cgagcgcctg
    16381 cgcgacggct tcgacccgac cgtgctgctc gcgcgcctcg gcggcgacgt gttcggcgcg
    16441 ctcggtccgg tcgaacggct cgatcccgaa agcgtcgccg ccctgttcgc cgacggtgcg
    16501 ccgtcggccg cggacggcgg cgcctggagc gtcggcaccg cgcgcctgcc gcttgcggag
    16561 ttcaccggca ccgcgctgga ggcgctggcg agcgccacgc tgctgctgaa gcaggccaag
    16621 ctgcgcggcc acggccagca tcaggactgg ctgccggaga tcgccgtcgc tgccggccgg
    16681 cgcttcgcgc tgctgcaggc gctgcgcgag gcgctcgcgc gcgacgagat cggcatcgag
    16741 ctgcagccgc agatcgacct cgcgaccggc accgtcaccg gcgtcgaggc gctggcgcgc
    16801 tggcagcgcg ccgacggcac gcgcgtcgca cccgatgcct tcatcccgct cgccgaggcc
    16861 accgggctga tcgtaccgct cggcgagcac ttcatcgagc ttgcctgcgc cgccgcggcc
    16921 cggctcgccg cggccggtca cggccacatc cgcgttgcgg tcaacgtctc ggccgtgcag
    16981 tcggcgcgcg ccgacctggt cgcgagcatc gagcgccagc tgctcgccag cggcgccgag
    17041 gcgcggcaga tggagatcga gctgaccgag accgtcgcga tgcagaactt cggcgccgtc
    17101 accgacacgc tcgaacgcct gcgcgcgcgc ggcctgaccg tcgccatcga cgacttcggc
    17161 accggcttct cgtcgctcgc ctacctgcgc cggctgccgg ccgacctgat caagatcgac
    17221 cgcagcttcg tgcaggaact cggcaacggc ggggaatcgc gcaccgagat cgccgaactg
    17281 atcatccggc tcgccgagcg cctgggcctg cgcgtgctcg ccgagggcgt ggagaccgag
    17341 gcgcaggccg gctggctgcg catgcacggc tgccattacg cgcagggctg gctgtatgcg
    17401 cgggcgatgc cgctcgatgc gctgctcgac tggctggcgc ggcgcgagca cgccggcacc
    17461 tgaaggttcc cccggcgccg ccgggagctt ccgcggcggc tgccggcgct ggccggcacg
    17521 caggcggatg actggccccg caacggtgca gcgcccgcgc cggtgtcgcc tcctgctggt
    17581 gcgtccggcg cgctacggac ggcacgacgc cgcgccagcc tggggccggc gcggcgtcgg
    17641 tggggaagac aggtggctca gtgcgggcgg tacggctgcg gcagctttct cggcggcttc
    17701 ggacgcaggt cgtaggtcgg gtgattggcc tcgaagcgtt cgagcacgcc gttcagctcg
    17761 gcgacccgcc ccacccggcc catgacctgg gcgtgggcga tgtcctccat gatgtagcgc
    17821 cggatcagcc actgtccggc gtgtgtctgc accagacact ggccgagttc ggcggcgatg
    17881 acttcgggaa catgctcatg ttcggcgatc gcctgaatct cttcctcgga cagctcgcac
    17941 aggtccagac attcctgctc ggtcagcatg gggcgactcc tctgtggtgg tgagggcgtt
    18001 ctgcgacgcc ttgtcatggt gcaggctgct ccgccgtgat gcggggcgtt actgcaattc
    18061 tttgaacatc cgcggcaaca ttccgggaat atcctcccgg tgcgggtgct tccggggctg
    18121 ctctgcagca gcaggtctgg cctcggggac cacaacggta gttcagtgaa tcagaagccg
    18181 ggccggcgtg gggtgatact ttggtaaggt ctgcgcccgg ggtttcagac cggtgcaagc
    18241 gtcgtgccgc aggcgcagcc aggggtttac ttcggccggc cgttccggtc taatgcgcgc
    18301 ctcgccgggc cgttagctca attggtagag cagttgactc ttaatcaatt ggttgtaggt
    18361 tcgagtccta cacggcccac cacacattct ggccgccttc gatgacgatt gcaggtcagt
    18421 acggacaagg ggctgcggtg ccgccgcagc cccttttcgt tgttgcggcc gcgcgggttc
    18481 agcccgccgg cggctccggg cgcaccggca gcaggccacg cgcttcggcg gcggcgagcc
    18541 aggcggcggc gcgcagcgcg agctgcgtgc ggttgctgac gcgggtcttg ccgaggatct
    18601 gcgagacgta gagcttgacg gtgttctcgg tcaggccgag gtcgcaggcg atcacgtagt
    18661 tggtcttgcc ggcgccgacg tgcgcgagca cctcccacat gcgcggcgtc agttcgggca
    18721 gcggctcgct gtcggacagc gcggctgcgg tcgtggccgg cgccgcttcc ggtggtgcgg
    18781 cttcgagccg ccggcgcagg ccgctgactt cctcgccgag gcgcagcacc gtatccatca
    18841 tctcgtcgag ccgcgcctcc agccgctcgc cgccggggct gcgccgcgcc aggcgcacgg
    18901 cggcacggcg cacggcgtcc agcagctcgt cgccggacag cggcttgttc aggaagtcga
    18961 tcgcgccgcg gcgcagcgcc tcgatcacgt cctccttgcc ggcatagccg ctgaccagga
    19021 tggcctcgaa ggcgcgcgtg tcgccgaaat cgagcccgag gcggccgacc agttcgttgc
    19081 cgttgccgat ggcgaggttc aggtcggtga tgacgatgcc gattcctgca tccgccgcga
    19141 acgtcgcgag ggcgtcgtcg acgccggtgg cgccgtggca gcggtaaccg ttcaggtcca
    19201 ggaattcgag cagctcctgc agcgcttcgg gttcgtcgtc gacgaccagc acggccggaa
    19261 cctgcggcgc tgagtcttgc ttcaccgggc ggaatcctcg attgacggtg ccggaaggac
//
LOCUS       DAOQIE010000026        19091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1304_length_19091_cov_7.693581, whole genome shotgun sequence.
ACCESSION   DAOQIE010000026 DAOQIE010000000
VERSION     DAOQIE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 19091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19091
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_19091_cov_7.693581"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..596
                     /gene="flgM"
                     /locus_tag="PK144_03080"
     CDS             267..596
                     /gene="flgM"
                     /locus_tag="PK144_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNF64738.1"
                     /translation="MTIDKLGGNGTPPVRGGPPVARVQSGADPAATSRTGGSAPATDA
                     VSLTPQSRQLREVESKLSETPAFDSARVAELRKAIERGEYRVDSRRVAGKLLEFERDL
                     SAPGAGD"
     gene            598..1071
                     /locus_tag="PK144_03085"
     CDS             598..1071
                     /locus_tag="PK144_03085"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNF64739.1"
                     /translation="MLTVLIEQELAATRALLAVLEQEYSALRERHDADTLEAVVRAKE
                     TAAGRLQQIAAGRMQHLLGSGYPPDAAGLAACIAAVPTEDRFRLNALWRELLDVAGRA
                     WKQNGINGAVIAASRSATERALALLRGQDPNTCLYGADARARYATGGSVRPLISA"
     gene            1146..1754
                     /locus_tag="PK144_03090"
     CDS             1146..1754
                     /locus_tag="PK144_03090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNF64740.1"
                     /translation="MSVTDSSPPEDRRQYFRVADEVVLAWARVDGDGLAALAERLRGE
                     GSEGYGFGPLYAALTQDAQGLLAQLRSEQPLVAQYTENLERRLDILASAIVLADMGVH
                     ERSARHVNLSAGGIEFEAETALETDATLELRFVIHPNRSVIVAGARVARCLPADPRSA
                     WPWRIAVEFICLREQDRQMIVRHVMFREARHLRERQPFDDGI"
     gene            complement(1759..2013)
                     /locus_tag="PK144_03095"
     CDS             complement(1759..2013)
                     /locus_tag="PK144_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64741.1"
                     /translation="MSGLILRLPWWLHLLMAVLMWPLFTEALPRLPVHEAWQLTLLRE
                     WAPRGWPLLSAGCVLMALVSARRAAGRRGAKPVSRGRRRG"
     gene            complement(2240..3181)
                     /locus_tag="PK144_03100"
     CDS             complement(2240..3181)
                     /locus_tag="PK144_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNF64742.1"
                     /translation="MTGGSAVLLAALGSAQAAGVSAGVPAMATLLAAPVAQQVEWGQR
                     FEHGEGVGRNAGRALTLYCAAAGRGSVDAQYRLGWMYAMGRGVARDDALAAAWFRQAA
                     AKGDPQAQQMLKAIGKVAVKTASCRAPAAQLQSVAYRGAATPRPLDLDTPERRQIVAW
                     VNELAPQYQLDPALVLAVITAESNFRADAQSTASAMGLMQLIQATADRFGVQNVWDPQ
                     QNLRGGMAYLRWLLAYFRGDVQLALAGYNAGENAVDRNGGIPPYAETQNYVARILQQY
                     GRMWHPHVEGLTEPSALVAALPPGQGPVRTTPVAAGR"
     gene            complement(3293..5725)
                     /locus_tag="PK144_03105"
     CDS             complement(3293..5725)
                     /locus_tag="PK144_03105"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF64743.1"
                     /translation="MSHRFVRTAWRTLAVLTALLALGIAGGWYWLRGSLPPLAGELSL
                     PGLQAPVSIVRDAAAVPHIRAQNVHDAWFALGVVHAQDRLWQMEMSRRIAGGRIAEAL
                     GATALDTDRLLRTMGLRRVAERNYRNLDAESRAVLDAYAAGVNAWIAAHGGPLPPEFL
                     LTGVRPEAWQPADSLSWLKVMALDLGSNWSGELTRLRLARQLPTARIQDFLPPVPYAG
                     PLPVADYAALYRGLDLPLAALGPQPRTDTDGLGSNNWVVAGSRSASGKPLLANDPHLG
                     LTAPAIWYFAHLEAPGLRVIGATLPGVPMVVIGRNERIAWGLTNTNPDVQDLYVERSD
                     PADPGRYQTPDGWQRFEQREEILHVRNAPDERLIVRTSRHGPLLPDTRTGDALPAGYG
                     LALAWTALAEDDASFRAASKLAYAQDWTGFNEALREFVAPQQNFVYADVDGHIGFLSP
                     GRIPVRRPDNDLLGLAPAPGWEARYDWAGWVPYEELPRVFDPADGALYSANERIVPPG
                     YRWHLTDEWQPAYRAARIRELLAATPAHTPASFRQMHADIRSGLAADLLPKLLQRAAL
                     TDYPPAARGWHERLARWDYGMRVDAAEPLVFSAWYRALTRRIYADELGPEFERLWDQR
                     TTFMNNVLDDRDGQGRWCDDVRTAATESCAQQVVAALGEALDELHARFGSDESRWHWG
                     DAHPAVAEHRPFSKLAWLRPLFELRRPVGGDVSTLDVARFFINDAEAPYAARHVASLR
                     MVVDLADPDRSTFMHSSGQSGNPLSPHYADLLDRWTRVESLPMSMQARDYEAGALGTL
                     TLRPPAAVSGGQ"
     gene            6008..6961
                     /locus_tag="PK144_03110"
     CDS             6008..6961
                     /locus_tag="PK144_03110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNF64744.1"
                     /translation="MSPSAETAAAVPSTGTFEAVEPARAWTMVAGAIEQLCARSPAAV
                     PLAELAAAAGLSEGQFQRVFSAWAGISPKRFQQFLGKEYAKQALRAGQDVLNAALAAG
                     LSGPGRLHDLLVSAEAVSPGEWRAGGSGLTLCWGFADTPFGRACLTLAPRGLHRLAFA
                     DTADAELARLRADWPHARLVEDHTAAQAMADAVFAQPPAPGRALHLWVRGTQFQLRVW
                     EALLAVPPGRLVAYADLARALGRPGAARAVGGAVGANPVAVLIPCHRVIRAAGELGGY
                     HWGLTRKRALIGYEAARGAVEAVPPSVGAEAADLFPAGASD"
     gene            6961..7827
                     /locus_tag="PK144_03115"
     CDS             6961..7827
                     /locus_tag="PK144_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF64745.1"
                     /translation="MRTADLARLVALAAIWGASFVFLRVLAPVLGAAWTAESRVLIAG
                     LALTAWFRLTGFAPGWRRNAKVFVVVGLLNSALPFVLYGWAAQHLPAAVMAVVNACTP
                     LSGAVCAAIWLGEPLTRTRLGGIGCGIAGVAWLSFSRPAGGPLPDPQLLPAIAACLLA
                     TLCYGLAGVYLKRCASAVAPLAIAGGSQLAAALWLLPLLPFAAPVGTLTGPVLASLLG
                     LGLICSALAYGLYYRLIADLGPTRALTVTFLIPPFGVAWSALLLGEPLSPALAGSGVL
                     IVAGTVLVLRGR"
     gene            complement(7837..10791)
                     /locus_tag="PK144_03120"
     CDS             complement(7837..10791)
                     /locus_tag="PK144_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64746.1"
                     /translation="MPVADSPQRPATRALPVWLWRAYLRGALWPLLIIELLLFVLAAV
                     IGHMASQALDAAWEARVREALALQAELEARALAQRLGSIAETAALLAERATPAEAADA
                     DRRLGRLLAREPALAGLQLLEEGAAPRLLTAATEPPAAPAVWRMQSGDGAPVAETRIP
                     LPEAGRELRLRVRLETLLAAGAWPAEVWLVAADGQVLARLRDEARLRDVLPPEVVQLA
                     SGTLRAPSDRLFAWSPVGTGGWRLVLRQPLDAISAPSTLLLPGIWLLAGLAGSFLLLF
                     WQLRRRARRISGQMVRPLRAIEGLVRRIGRGDYTPPPPKLALLELQDTADAVLEMGRR
                     LGEANCLLVEQGRDLRRQAGFRQLLIDSVPVPLFYTDAGGRLLGGNLAFRAFVGTRSD
                     GGEAMARRLLQALPEAAPGLPAAIETLFDDGLDGPRHLLVSGAGFSSVDEADGGQVGV
                     ILDLTERRRGERELEAARDRALAASRQKSDFLASVSHEIRTPMNGVIGMTELLLHTGL
                     SAEQHDYARTILGSAQALLQLLNDILDFSKIEAGQITLAADPFDPATLVEGAAELLAA
                     RAWEKRIVLATRVDPRLPVPLYGDAGRVRQVLLNLIGNAVKFTDAGEVIVAAEALSHD
                     GRQALVRFSVTDTGLGIPDSARPRMFRPFSQLEGQGERRYAGTGLGLSICKRLCELMG
                     GDISFDSEPGRGSVFRVTLPFRRTGELALLEAGTGPVPAGSTALPVLPPAGVLLDGLP
                     AGAHAALAGTLRAFGLSVYDDQGIEAMAPALRLVLCGAAPEALRAQAAYPSLPAVLLA
                     TRDEAGLAARAEAQGWSAVLALPLRRAALHEILGALLYGRSGSGTAPNGTLPARAAVV
                     PRGATLLLAEDDRTNQRVASAHLRKLGFAVEVVGDGDAAVKAATGRHYPLILMDVQMP
                     VMDGLQAAAAIRRAQAGGPASIIVALTANAMSGDRERFLAAGMDDYLSKPFTGDALRR
                     LLERWLPPEGEG"
     gene            complement(10822..11226)
                     /locus_tag="PK144_03125"
     CDS             complement(10822..11226)
                     /locus_tag="PK144_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNF64747.1"
                     /translation="MQPNYGPTYYGGYQTRMTQTVQFGVVEGVRPVVIADRYNSGVGA
                     LTGAAIGGIAGSAIGQGRGAAIAAIGGALLGGLAGNAIEGQSAAQTGLELTVRLDSGR
                     IIAITQGAGEPFYRGDRVRVLSAPDGSARVSH"
     gene            complement(11330..13756)
                     /locus_tag="PK144_03130"
     CDS             complement(11330..13756)
                     /locus_tag="PK144_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64748.1"
                     /translation="MNALPMSPAAAGQDGEHLWIDNLILAVLVLAGGWIAARVPMPGG
                     LPLPFWPPAGVVLAGLLCFGWRALPGLVIGTLLLFYDLTFYQWRPLPILIAGAGLIAQ
                     GAAGAWLVRRVQGGALAFTRAQDFGLFLLLGGLLPSLLGSSAWLTGFGLSLTQYWPTA
                     ILAQLSGVAIVAPIVFAWALRAQPAWQGRMRTVALPLVLVTALLVALLNPLLGPMDAD
                     TRELLRVAHIHAAWWLMSAGLFVCALLAVLLLQLTARSAAVRALVEQRTAELERANAE
                     LAREIDVRKRTELRLREAMEEAASAGRLKNEFLGNMSHELRTPLNAVIGMTELALDAE
                     LPGEVRAYLEAARSGAGELLTLIDGMIDFSQMSRGGLQIEATRYRLRQLLSGPLKAAG
                     ERARAKGLVFQVRIEEALPDLLIGDPGRLRQIVQHLTDNAVKFTAHGGIEVRVSELPG
                     TVGARLLSIEVVDTGIGVPEDHREAIFDPFHQVDASTTRRFGGAGMGLSICRQLARLM
                     GGELELAESTEGRGSRFALTLPLRDREPVAGETPSALVVADAIGNRRMLTEVMSKQGL
                     AVITASDAAEGLALLERGRVDGQPYRLLLIDIDESVEYAFRMVRTLRELQGAAAPSIF
                     ILTGHGIRGDAARSRELGVAAYLTKPVTPAELREAVAIVLAGPGFDALVTRHSLRERR
                     MATPLILGVGVDAHPRELVAALEEHGCRATAVCSAPSLRTLCAEREVEAIVLDWDAPE
                     LHAPEVVEALLEREHLGRHRPRIIACAVTALEVPGVDIWLPAPLELPALLAALGRAPA
                     RAGMPERELS"
     gene            complement(13753..>14418)
                     /locus_tag="PK144_03135"
     CDS             complement(13753..>14418)
                     /locus_tag="PK144_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HNF64749.1"
                     /translation="ACFSLCCGGYVVLAGQRRASRRSLLQATYRHRRVWMERMLERDN
                     RIFDSTLLGNLMRSVSFFASTTIIVIGGVFAMFGSADRAVAATADLPFVAHASRELWQ
                     IKLVVLGVLFVYAFFKFTWSLRQFNYCSIMMGAAPMPGDFPDRASLDRCAEGLARLNS
                     LAGDQFNQGLRAYYFGLAVMSWLIHPLLFAPVSVLTVAVLYRREFNSTTLDALAYQYE
                     AES"
     assembly_gap    14419..14518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14582..16039)
                     /locus_tag="PK144_03140"
     CDS             complement(14582..16039)
                     /locus_tag="PK144_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNF64750.1"
                     /translation="MPSVNIWKRARDGWPHWWSAVLDRLNPAATPTAATPEPGAPGMA
                     GPSASAADTHLGLARDSLRALLADEAVPAAVRERLAEDYSALSLLLERLEHGHLHIAV
                     FGRVSVGKSALLNALLGEQRFATSPLHGETTAASSARWAADPELPGVQRLEDGGVFLI
                     DTPGIDEVGGEARERLAREVAGGADLILFVCEGDLTATELNALRELAAEGTRPIVLAL
                     NKTDRYTRAERALLLEALETRSRGLVRPEHIVPCAAAPAERTVIRVDAGGREHESRER
                     PAPDIEDLRETLWDILAAEGKTVAALNAGLFAGRFCERLAREVVELRRSLAETVIRRY
                     CLAKGLGVALNPVPLADLAAVFADVAMIVHLGRVYGLPVSHTEAGALLRTLIAQLALL
                     MGSSYLASLASSALKGATFGLSTIVTAGAQGAVAWYGTYVVGLAAQRYFAQGRSWGEG
                     GPKRVVREILASLDRDSLLAEARADILARLQGDPR"
     gene            complement(15930..17402)
                     /locus_tag="PK144_03145"
     CDS             complement(15930..17402)
                     /locus_tag="PK144_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNF64751.1"
                     /translation="MRRWRPLLFALGLALTLALALHVTDFALTLWQRLSEASPLFLTL
                     YGLLIALVAWLVLRLMRPRRPVAAAAPPPPDAPQIDRQLEAAVQAGLPVERYRAELAE
                     LERRRAGGRVIVACFGEVSAGKSSLIRALLPGAEVVVSPVGGSTRTIVHHVWTSPAGD
                     ELVLADVPGLNEADGTLDAEAEGEAVRAHAVIYVTGGDLARAELAALERLLSLGKPLL
                     VALNKADRYTAAELALVQARIRERLAGRAELVAVSAGGTEEVVRIGADGSETRIERER
                     PADVHALGVALQRTLDADAGALESLRDAAVFTLIGRELDAALAAERAARAETVVETYA
                     RKAVFGALAAVGPGTDVLIQGWLGMQMLKELSAVYGVPAQEIDLKRFLDLASRHVGRR
                     MNLLLALAGNVLKAFPGVGTVLGGALHAIAYGLIFESLGHAAARSLASRGALVPGVAV
                     HLFEDALGEHLETRARRLAALVVGRARSAEPGRDADRSDA"
     gene            complement(17410..17847)
                     /locus_tag="PK144_03150"
     CDS             complement(17410..17847)
                     /locus_tag="PK144_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF64752.1"
                     /translation="MARVKLDLPATFAFRTSMQVRVGDINYGRHLGNDALLGLLHEAR
                     VRWLAAHGQSELDCWGAGLIMVDSVIVYRAEAFLGETLLFDLATADVHGKGCDLVYRV
                     SAAGDGRDIAHAKTGILFFDYATRRVRAMPEEFRSALCAAAGA"
     gene            complement(17953..18429)
                     /locus_tag="PK144_03155"
     CDS             complement(17953..18429)
                     /locus_tag="PK144_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HNF64753.1"
                     /translation="MLAEPLDDEMTTIADEIHDKALPKLVERLRLERQALADRHETLS
                     CGCAQLGIAELLPQLEALCQQLVDYVALGHFEVYQLMRSCPGRGLPACASTRALLEEI
                     EAALADTAQAAVDFNDRYGGEHTCSFERLAADLARLGATLAARTALEERLFAACTA"
     gene            complement(18567..>19091)
                     /gene="tsaB"
                     /locus_tag="PK144_03160"
     CDS             complement(18567..>19091)
                     /gene="tsaB"
                     /locus_tag="PK144_03160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNF64754.1"
                     /translation="WSALDGIAFGRGPGAFVGVRIATACAQGLAFAHDLPVAPVSTLE
                     TLAYGAGRRQGARQVFAAIDARMGEIYAAAWPLDADGWPQEPRLAECVTAASALSLPG
                     TWFGVGSGLAAAAAAFDGRIAFSDRDPAALPHAADVARLGRRLLLAGAGVSAAAAQPV
                     YLRDEVAKKSAVRG"
BASE COUNT         2704 a         6948 c         6894 g         2445 t
ORIGIN      
        1 cgtgcgccgc ggtgcgatgc gcatcagcgc cggcggcgtc gcactcgccg acggccgccc
       61 cggcgagcgc ctgcgcgcgc gcaacctcgc ctccgggcgg gtcgtcgaag gccggctgga
      121 gccggagcgc acgctgatcg tcgactgaac cccggagcga ccgacaccgg gcgtaacgct
      181 ctgccggaaa acccgattca agttccggcg gccggggccg atacggagtt ccgtatactc
      241 gcgattccga cgagaggacg actccgatga ccatcgacaa gctgggcggc aacggtaccc
      301 cgcccgtccg cggcggcccg ccggtcgcgc gcgtgcagtc tggcgccgac cccgcggcca
      361 cctcccgcac cggcggcagc gcgccggcca ccgacgcggt cagcctgacc ccgcagtccc
      421 gccagctgcg cgaggtcgaa tcgaagctct ccgaaacccc ggcctttgat tcggcgcggg
      481 tggcggagct gcgcaaggcg atcgaacgcg gcgaataccg cgtcgacagc cggcgtgtcg
      541 ccggcaagct gctggagttc gagcgtgacc tgtccgcccc gggtgcagga gactgacatg
      601 ctgaccgtgc tgatcgagca ggaactcgcc gcgacgcgcg ccctgctcgc cgtgctcgaa
      661 caggaatact cggcgctgcg cgaacgccac gatgccgaca cgctcgaggc cgtcgtgcgc
      721 gccaaggaaa ccgcggccgg acggctgcag cagatcgccg ccgggcgcat gcagcacctg
      781 ctcggcagcg gctatccgcc cgatgccgcc ggcctcgcag cctgcatcgc cgcggtgccg
      841 accgaggatc gcttccggct gaacgcgctg tggcgcgaac tgctcgatgt cgccggtcgc
      901 gcctggaagc agaacggcat caacggcgcg gtgatcgctg ccagtcgcag cgccaccgaa
      961 cgcgcgctgg cgctgctgcg cggccaggac ccgaacacct gcctgtacgg cgccgacgcc
     1021 cgcgcccgct atgccaccgg tggcagcgtg cggccgctga tcagtgcctg aaacccgtcg
     1081 cgcatgacga cgcggggcag cggatgcgat gatccgccgc ccccttccgc cggaacatcc
     1141 agtcgatgag cgtcacagat tcgtcgccgc ccgaggatcg ccgtcagtat ttccgcgtcg
     1201 ccgatgaagt cgtgctcgcc tgggcacgcg tcgatggcga cgggctggcc gcactcgccg
     1261 aacgcctgcg cggcgaaggc agcgaaggct acggcttcgg accgctgtac gcggcgctga
     1321 cgcaggacgc gcaggggctg ctcgcgcagt tgcgcagcga acagccactg gtcgcgcaat
     1381 acaccgagaa cctcgaacgc cggctcgaca tcctcgcgag tgccatcgtg ctcgccgaca
     1441 tgggcgtgca cgagcgcagc gcccgccacg tcaacctgtc ggcgggcggc atcgagttcg
     1501 aggccgagac ggccctggaa accgatgcga cgctggagct gcgcttcgtg atccatccga
     1561 accgctcggt gatcgtcgcc ggcgcccgcg tcgcccgctg cctgcccgcc gatccgcgca
     1621 gcgcctggcc gtggcgcatc gccgtcgaat tcatctgcct gcgcgagcag gaccgccaga
     1681 tgatcgtgcg ccacgtgatg ttccgcgaag cccgtcacct gcgcgaacgc cagcccttcg
     1741 acgacggaat ctgagccttc agccccggcg ccggccgcgg ctgaccggct ttgcgccacg
     1801 gcggccggcg gcgcggcgcg cactgacgag cgccatcagc acgcagccgg ccgacagcag
     1861 cggccagcca cgcggtgccc attcgcgcag cagcgtcagc tgccaggcct catgcaccgg
     1921 caggcgcggc agggcttcgg tgaacagcgg ccacatcagc accgccatca gcaggtgcag
     1981 ccaccacggc aggcgcagga tcaggccgct caagccatcg tccgcgtagc gcggcgtgcg
     2041 ccgccgtgcc gggacagcag cgccggcgcg gggttggcgg cgggcggtgg tgcgaggctc
     2101 ggccggggtc ttcggcgggg tcgagggatc agcagacggg cggcgcggca tgctcgggct
     2161 ccggtcgggc ggccggcgca gcgtagaccg ccgtgcccgc ccgcgcccgt cgccgtcgcg
     2221 gccgcgatac cgccggggct cagcgcccgg ccgcgaccgg cgtcgtgcgc accgggcctt
     2281 gtcccggcgg cagcgccgca accagcgccg agggctcggt cagcccctcg acgtgcggat
     2341 gccacatgcg gccgtactgc tgcaggatgc gggcgacgta gttctgggtc tcggcgtagg
     2401 gcgggatgcc gccgttgcga tcgaccgcgt tctcgccggc gttgtagccg gccagcgcca
     2461 gctgcacgtc gccgcggaaa tacgcgagca gccagcgcag ataggccatg ccgccgcgca
     2521 ggttctgctg cgggtcccag acgttctgca cgccgaagcg gtcggcggtc gcctggatca
     2581 gctgcatcag ccccatcgcg ctcgccgtcg actgcgcatc ggcgcggaag ttggattcgg
     2641 cggtgatcac cgccagcacc agcgccggat cgagctggta ctgcggcgcc agttcgttga
     2701 cccaggcgac gatctgccgg cgctcgggcg tgtcgagatc gagcgggcgc ggcgtcgcgg
     2761 cgccacgata tgcgaccgac tgcagctgcg cggccggcgc gcggcagctc gccgtcttca
     2821 ccgccacctt gccgatcgcc ttcagcatct gctgcgcctg cggatcgccc ttcgccgccg
     2881 cctgccggaa ccaggccgcg gccagcgcgt cgtcgcgggc cacgccgcgg cccatcgcgt
     2941 acatccagcc gagccggtac tgcgcatcga ccgaaccgcg accggcggcc gcgcagtaca
     3001 gcgtcagcgc gcgcccggcg ttgcggccga caccctcgcc gtgctcgaag cgctgtcccc
     3061 attcgacctg ctgcgccacc ggtgctgcca gcagcgtggc catggccggc acgccggcgg
     3121 acacgccggc tgcctgcgcg ctgccgagtg cggcgagcag cacggcgctt ccccctgtca
     3181 agcgtcggat catcgtcgtg acttcacacc agaaaccggt tcggccgcat gttaacggcg
     3241 cccgccgacc cggcacagcc gctgccaccg gaagatacag cgcaagcggg gttcactgac
     3301 cacccgacac cgcggccggc ggtcgcagcg tcagcgtgcc gagcgcgccg gcctcgtagt
     3361 cgcgcgcctg catcgacatc ggcagtgatt cgacgcgcgt ccagcgatcg agcagatcgg
     3421 cgtagtgcgg cgacagcgga ttgccgctct ggccgctcga atgcatgaag gtcgagcgat
     3481 ccggatcggc gaggtcgacg accatgcgca ggctggcgac gtggcgggcg gcgtacggtg
     3541 cctcggcatc gttgatgaag aagcgcgcga catcgagcgt cgagacatcg ccgccgaccg
     3601 gccggcgcag ctcgaacagc ggccgcagcc aggcgagctt cgagaacggc cggtgctcgg
     3661 cgacggccgg atgcgcgtcg ccccagtgcc agcgcgattc atcgcttccg aagcgcgcgt
     3721 gcagctcgtc gagcgcctcg ccgagcgccg cgaccacctg ctgcgcgcag gactcggtcg
     3781 cggccgtgcg cacgtcatca caccagcgcc cctgcccgtc gcgatcgtcg agcacgttgt
     3841 tcatgaaggt cgtgcgctgg tcccacagcc gctcgaactc gggcccgagc tcgtcggcgt
     3901 agatgcgccg ggtcagcgcc cggtaccagg ccgaaaacac cagcggctcg gcggcatcga
     3961 cgcgcatgcc gtaatcccag cgcgccagcc gctcgtgcca gccgcgcgcc gccggcgggt
     4021 agtcggtgag cgctgcgcgc tgcagcagct tcggcagcag gtcggcggcg agccccgagc
     4081 ggatgtcggc gtgcatctgc cggaagctcg ccggcgtgtg cgccggcgtc gcggccagca
     4141 gctcccggat gcgcgcggcg cggtaggccg gctgccattc gtcggtcagg tgccagcggt
     4201 agccgggcgg cacgatgcgt tcgttggcgc tgtagagcgc gccgtcggcc ggatcgaaca
     4261 cgcgcggcaa ttcctcgtac ggcacccagc cggcccagtc gtagcgcgcc tcccagcccg
     4321 gcgccggcgc cagcccgagc aggtcgttgt cgggcctgcg caccgggatg cgccccggcg
     4381 acaggaagcc gatgtgaccg tcgacatcgg cgtacacgaa gttctgctgc ggcgcgacga
     4441 actcgcgcag cgcctcgttg aagccggtcc agtcctgcgc gtacgcgagc ttcgaggcgg
     4501 cccggaagct cgcgtcgtcc tcggcgagcg ccgtccacgc cagcgccagc ccgtagccgg
     4561 ccggcagcgc atcgccggtg cgggtgtccg gcagcagcgg gccgtggcgg ctggtgcgca
     4621 cgatcagccg ttcgtcgggc gcgttgcgca catgcaggat ttcctcgcgc tgttcgaaac
     4681 gctgccagcc gtccggcgtc tggtaacgcc ccggatcggc cggatcgctg cgttcgacat
     4741 aaagatcctg cacgtccggg ttggtgttgg tcaggcccca ggcgatgcgc tcgttgcggc
     4801 cgatcacgac catgggcacg ccgggcagcg tcgcgccgat cacgcgcagg cccggggctt
     4861 cgaggtgcgc gaagtaccag atcgccggcg ccgtcagccc gaggtgcgga tcgttggcga
     4921 gcagcggctt gccgctggca ctgcggctgc cggcgaccac ccagttgttc gagcccaggc
     4981 catcggtatc cgtgcggggt tgcggaccga gggcggcgag cggcaggtcg agcccgcggt
     5041 acagcgccgc gtagtcggcg accggcagcg gtccggcgta cggcaccggc ggcaggaagt
     5101 cctggatgcg cgcggtcggc aattgtctgg cgagccgcag gcgcgtcagc tcgccgctcc
     5161 agttgctgcc gagatcgagc gccatgacct tcagccagga cagcgaatcg gccggctgcc
     5221 aggcctcggg gcgcacgccg gtcagcagga attcgggcgg cagcggcccg ccgtgcgcgg
     5281 caatccaggc gttgacgccg gcggcgtagg catcgagcac ggcgcgggat tcggcatcga
     5341 ggttgcggta attgcgctcg gcgacacgcc gaagccccat cgtgcgcagc agccggtcgg
     5401 tgtcgagcgc cgtcgcgccg agtgcctcgg cgatgcgccc gccggcgatg cgccgcgaca
     5461 tctccatctg ccacagccgg tcctgcgcat gcacgacgcc gagggcgaac caggcatcgt
     5521 ggacattctg cgcacggatg tgcggcacgg ccgcggcgtc gcgcacgatg ctcaccggcg
     5581 cctgcaggcc gggcaggctc agttcgccgg cgagcggcgg cagcgagccg cgcagccagt
     5641 accagccgcc ggcgatcccg agtgcgagca gcgccgtcag caccgccagc gtccgccacg
     5701 ccgtgcgcac gaatcgatgc gacatcccca tgaccgatcc cctcttccgg ccgctgccgc
     5761 ggcgaaagtc cgcgatgatg cacatccggc cggacgcgcg tcaccgcagc ttcatgacag
     5821 tttcatcacc ccgtcatgtt cctgcgctgc gacgagccac atggaggcag ccaccggccg
     5881 ggcgtgcaca aaaaatcccg gtgcagcctc cggaccggct gcgggcgaag ccgcaggaat
     5941 cgggtgggcg gaccgccggg cagcggccag aatgccgccc gcccgtcccc gtccgcggag
     6001 agcccgcatg tccccgtctg ccgaaaccgc tgccgccgtc ccgtccaccg gaaccttcga
     6061 agccgtcgaa cccgcgcgcg catggacgat ggtcgcgggc gcgatcgagc agctctgcgc
     6121 gcggagcccg gccgcggtgc cgctcgccga gctggcggcg gcggccggcc tgagcgaagg
     6181 ccagttccag cgcgtgttct cggcctgggc cggcatcagc ccgaagcgct tccagcagtt
     6241 tctcggcaag gaatacgcga agcaggcgct gcgcgccggg caggacgtgc tgaacgccgc
     6301 gctcgccgcc ggcctgtcgg gtccgggccg gctgcacgat ctgctggtca gcgccgaagc
     6361 cgtgtcgccc ggcgaatggc gcgccggcgg cagcggcctg acgctctgct ggggtttcgc
     6421 cgacacgccg ttcggccgcg cctgcctgac gctcgcgccg cgcggcctgc accggctggc
     6481 gttcgcggac acggccgatg cggaacttgc gcgcctgcgt gccgactggc cgcacgcacg
     6541 gctggtcgag gaccacaccg ccgcgcaggc gatggccgat gcggtgttcg cacagccgcc
     6601 cgcaccgggc cgcgcgctgc acctgtgggt gcgcggcacg cagttccagc tgcgggtctg
     6661 ggaggcgctg ctcgcggtgc cgccggggcg gctggtggcc tacgccgacc tggcccgcgc
     6721 cctcggccgc cccggtgcgg cgcgcgcggt cggcggcgcg gtcggcgcca atccggtcgc
     6781 ggtgctgatt ccgtgtcatc gcgtgatccg cgctgccggc gagctcggcg gctaccactg
     6841 ggggctgacg cgcaagcggg cgctgatcgg ctacgaggcg gcgcgcggcg cagttgaagc
     6901 agtgccgccc tctgtcggag ccgaagcggc agacctcttc cccgccggag ccagcgactg
     6961 atgcgcaccg ccgatctcgc gcgcctcgtc gcgctcgcgg cgatctgggg cgcgtccttc
     7021 gtgttcctgc gcgtgctggc gccggtgctc ggtgcggcgt ggacggccga atcgcgcgtg
     7081 ctgatcgccg gcctcgcgct gaccgcgtgg tttcgcctca ccggctttgc acccggctgg
     7141 cggcgcaatg caaaggtgtt cgtcgtcgtc ggcctgctga attcggcgct gccgttcgtg
     7201 ctgtacggct gggccgcgca acacctgccg gcggcggtga tggcggtcgt caatgcctgc
     7261 acgccgctgt ccggcgccgt gtgcgcggcg atctggctcg gcgagccgct gacccgcacc
     7321 cggctcggcg gcatcggctg cggcatcgcc ggcgtcgcgt ggctgagctt ctcccgcccg
     7381 gcgggcggcc cgctgcccga cccgcagctg ctgccggcga tcgccgcctg cctgctggcg
     7441 acgctctgct acggcctggc cggcgtctac ctgaagcgct gcgcgagcgc cgtcgccccg
     7501 ctcgcgatcg ccggcggcag ccagctcgcg gcggcactgt ggctcctgcc gctgctgccg
     7561 ttcgccgcgc ccgtcgggac gctgaccggc ccggtgctcg caagcctgct cggcctcggc
     7621 ctgatctgca gcgccctcgc ctacgggctc tattaccgcc tgatcgccga cctcggcccg
     7681 acgcgcgcgc tgaccgtgac cttcctgatc ccgcccttcg gcgtcgcgtg gagtgcgttg
     7741 ctgctcggcg aaccgctgtc gccggcgctg gccggcagcg gcgtgctgat cgtggcgggg
     7801 acagtgttgg tgctgcgcgg acgctgaggt tcgcgctcag ccctcgcctt ccggcggcag
     7861 ccagcgctcc agcagccggc gcagcgcgtc gccggtgaag ggcttgctca ggtagtcgtc
     7921 catgccggcg gcgaggaagc gttcgcggtc gccggacatg gcgttggcgg tcagcgcgac
     7981 gatgatcgag gccgggccgc cggcctgcgc gcggcggatt gccgcggcgg cctgcaggcc
     8041 gtccatgacc ggcatctgca cgtccatcag gatcagcggg taatggcgcc cggtcgccgc
     8101 cttgaccgcg gcatcgccgt cgccgaccac ctcgaccgcg aagccgagct tgcgcaggtg
     8161 tgcgctggcg acgcgctggt tggtgcggtc gtcctcggcg agcaagagcg tcgcgccgcg
     8221 cggcacgacc gcggcacgtg ccggcagcgt gccgttgggg gccgtcccgc tgccgctgcg
     8281 gccgtacagc agcgcgccca ggatttcgtg cagcgccgcg cggcgcagtg gcagcgccag
     8341 caccgccgac cagccctggg cttcggcgcg cgccgcgagt ccggcctcgt cgcgggtcgc
     8401 gagcagcacg gcgggcagcg acggatacgc ggcctgtgcg cgcagcgcct cgggcgcggc
     8461 cccgcagagc accagccgca gcgccggagc catcgcctcg atgccctggt cgtcgtacac
     8521 gctcaggccg aacgcacgca aggtgccggc gagcgcggca tgggcgccgg ccggcaggcc
     8581 gtcgagcagc acgccggccg gcggcagcac cggcagcgcg gtggacccgg ccggcaccgg
     8641 gccggtaccg gcttcgagca gcgccagctc accggtgcgc cgaaacggca gcgtcacccg
     8701 gaacaccgag ccgcgccccg gctcggagtc gaagctgatg tcgccgccca tcagctcgca
     8761 gagccgcttg cagatcgaca gccccagccc ggtgccggca tagcggcgct cgccctggcc
     8821 ttcgagctgg ctgaacggcc ggaacatgcg cggcctcgcc gagtcgggga tgccgaggcc
     8881 ggtatccgtg accgagaagc gcaccagcgc ctggcggccg tcgtggctca acgcctcggc
     8941 ggcgacgatg acctcgccgg catcggtgaa cttcacggcg ttgccgatca ggttcagcag
     9001 cacctgacgc acgcgcccgg cgtcgccgta cagcggcacc ggcaggcgcg gatcgacgcg
     9061 cgtcgcgagc acgatgcgtt tttcccaggc ccgcgccgcc agcagttcgg ccgcgccttc
     9121 gaccagcgtc gccggatcga agggatcggc ggcaagcgtg atctggccgg cctcgatctt
     9181 cgagaagtcg aggatgtcgt tcagcagctg cagcagcgcc tgcgccgagc cgaggatcgt
     9241 gcgggcgtaa tcgtgctgct cggcgctcag cccggtgtgc agcagcagct cggtcatgcc
     9301 gatgacgccg ttcatcggcg tgcggatctc gtggctgacc gaggccagga agtcggattt
     9361 ctgccgtgag gcagcgagcg cgcggtcgcg ggcggcttcg agttcgcgct cgccgcgccg
     9421 gcgttcggtc agatcgagaa tgacgccgac ctgaccgccg tccgcctcgt cgacgctgct
     9481 gaagccggct ccggatacca gcaggtggcg cgggccgtcg aggccgtcgt cgaacagggt
     9541 ttcgatggcc gccggcaggc cgggcgcggc ttcgggcagc gcctgcagca gccggcgcgc
     9601 catcgcctcg ccgccgtcgc tgcgcgtgcc gacgaaggcg cggaaggcca gattgccgcc
     9661 gagcagccgg ccgccggcgt cggtgtagaa cagcggcacc ggcacgctgt cgatcagcag
     9721 ctgccggaag ccggcctgcc ggcgcaggtc gcgcccctgc tcgaccagca ggcagttggc
     9781 ctcaccgagg cgccggccca tctccagcac ggcatcggcg gtgtcctgca gttcgagcag
     9841 cgcgagcttc ggtggcggcg gcgtgtagtc gccgcggccg atgcgccgca ccagcccttc
     9901 gatcgcgcgc agcggccgca ccatctgccc ggaaatccgc cgcgcccgcc ggcgcagttg
     9961 ccagaacagc agcaggaagc tgccggcgag cccggcgagc agccagatgc cgggcagcag
    10021 cagcgtcgag ggcgcgctga tcgcatcgag cggctggcgg agcaccagcc gccagccgcc
    10081 ggtgccgacc ggtgaccacg cgaacagacg atcactcggt gcgcgcagcg tgccggaggc
    10141 aagctgcacg acctcgggcg gcaacacgtc gcgcagccgt gcctcgtcgc gcaggcgcgc
    10201 gagcacctga ccgtcggcgg ccaccagcca gacctcggcc ggccaggcac cggcggcgag
    10261 cagggtttcc agccgcacgc gcaggcgcag ctcccggccg gcttccggca gcgggatgcg
    10321 cgtctcggcg accggggcgc cgtcgccgga ctgcatgcgc cagacggccg gcgctgccgg
    10381 cggctcggtg gccgccgtca gcagacgcgg ggcagcccct tcctccagca actgcaggcc
    10441 ggcgagggcc ggctcgcgcg ccagcagccg gccgagccgc cgatccgcgt cggcggcctc
    10501 ggcgggggtc gcacgttcgg cgagcagcgc agccgtttcc gcaatgctgc cgaggcgctg
    10561 cgcgagcgcg cgcgcttcca gctcggcctg cagcgccagc gcctcgcgga cgcgggcctc
    10621 ccaggcggca tcgagcgcct gactggccat gtgtccgatc acggcggcca gcacgaacag
    10681 cagcagctcg atgatcagca gcggccacag cgcgccgcgc aggtaggcgc gccacagcca
    10741 gaccggcagg gcgcgggtgg ccgggcgttg cggggaatcg gcgacgggca agatcgaagg
    10801 gtccgctcgg gcgatgaggt ctcagtgcga aacgcgggcg ctgccgtccg gtgcgctcag
    10861 cacgcgcacg cggtccccgc gataaaacgg ctccccggct ccctgcgtga tcgcgatgat
    10921 gcggccgctg tcgaggcgca cggtgagctc caggccggtc tgggccgcgg actgcccctc
    10981 gatggcgttg ccggcgagtc cgcctagcaa ggcaccgccg atcgccgcga tcgccgcccc
    11041 gcggccctga ccgatggcgc tgccggcgat gccgccgatc gcggccccgg tgagggcgcc
    11101 gacgccgctg ttgtagcgat cggcgatcac gaccgggcgc acgccttcga cgacgccgaa
    11161 ctgcacggtc tgcgtcatgc gcgtctgata gccaccgtag taggtcgggc catagttcgg
    11221 ctgcacgcag cctccgagca gcagcgcgat cccgagggaa gccagcgcga acggacggca
    11281 gcggcgacgg acatcaggct gaaaggcttt cattgctacg gttctccgtt caggacagtt
    11341 cccgctccgg catcccggcc cgggccggtg cccggccgag ggccgcgagc agcgccggca
    11401 gctccagcgg cgccggcagc cagatgtcga cgcccggcac ctcgagcgcc gtgaccgcgc
    11461 aggcgatgat gcgcggccgg tgccggccga ggtgctcgcg ttcgagcagg gcctcgacga
    11521 cttcgggcgc atgcagctcg ggcgcatccc agtcgagcac gatcgcctcg acctcgcgct
    11581 cggcgcagag cgtgcgcagc gatggcgccg aacacaccgc agtcgcccgg cagccgtgtt
    11641 cttccagcgc ggcgaccagc tcgcgcggat gtgcgtcgac gccgacgccg aggatcagcg
    11701 gcgtggccat gcgccgctcg cgcagcgaat ggcgcgtgac cagcgcatcg aaacccggtc
    11761 cggccagcac gatcgccacc gcctcgcgca gctccgccgg cgtcaccggc ttggtcagat
    11821 aggcggcgac cccgagctcg cgcgaacgcg ccgcatcgcc gcggatgccg tgaccggtca
    11881 ggatgaagat cgacggcgcc gccgcaccct gcagctcgcg cagcgtgcgc accatccgga
    11941 aggcgtactc gacgctctcg tcgatgtcga tcagcagcag ccggtagggc tggccgtcga
    12001 cgcggccgcg ttcgagcagc gccaggccct cggcggcatc gctggcggtg atgaccgcga
    12061 ggccctgctt gctcatcacc tcggtcagca tgcggcggtt gccgatcgca tcggcgacga
    12121 ccagggcgct cggggtttcg ccggcgaccg gctcgcgatc gcgcagcggc agcgtcagcg
    12181 caaagcggct gccgcgcccc tcggtgctct cggcgagttc gagctcgccg cccatcagcc
    12241 gcgcgagctg gcggcagatc gacaggccca tgccggcgcc gccgaagcgg cgcgtcgtcg
    12301 aggcatcgac ctgatggaag ggatcgaaga tcgcctcgcg atggtcctcg ggcacgccga
    12361 tgccggtgtc gacgacctcg atcgacagca gccgcgcacc gaccgtgccc ggcagttcgc
    12421 tgacgcgcac ctcgatgccg ccgtgcgcgg tgaacttcac ggcgttgtcg gtcaggtgct
    12481 gcacgatctg gcgcagccgt ccgggatcgc cgatcagcag gtccggcaac gcttcctcga
    12541 tgcgcacctg gaacaccagc cccttggcgc gcgcccgctc gccggcggct ttcaacggtc
    12601 cggacagcag ctgacgcagg cggtagcgcg tcgcctcgat ctgcaggccg ccgcggctca
    12661 tctgcgagaa gtcgatcatg ccgtcgatca gcgtcagcag ttcgccggcc ccggagcgtg
    12721 cggcttcgag ataggcgcgc acctcgcccg gcagctcggc atcgagcgcg agttcggtca
    12781 tgccgatcac ggcgttcagc ggcgtgcgca gctcgtggct catgttgccg aggaattcgt
    12841 tcttcagccg gccggccgag gcggcctcct ccatggcctc gcgcaggcgc agctcggtgc
    12901 gcttgcgcac gtcgatctcg cgcgccagct cggcgttggc gcgctccagc tcggccgtgc
    12961 gctgctcgac cagcgcgcgc accgcggccg agcgcgccgt cagctgcagc agcagcaccg
    13021 caagcagcgc gcacacgaac aggccggcgc tcatcagcca ccaggcggcg tggatgtgcg
    13081 cgacgcgcag cagctcgcgg gtgtcggcat ccatcggccc gagcagcgga ttgagcagcg
    13141 ccaccagcag cgccgtgacc agcaccagcg gcagcgcgac ggtgcgcatg cgcccctgcc
    13201 aggccggctg cgcgcgcagg gcccacgcga acacgatcgg cgcgacgatc gccacgccgc
    13261 tgagctgcgc gaggatcgcg gtcggccagt actgcgtcag cgacagcccg aaccccgtga
    13321 gccaggcgct gctgccgagc agcgacggca gcaggccgcc gagcagcagg aacagcccga
    13381 agtcctgcgc ccgcgtgaac gccagtgcgc cgccctgcac gcgacgcacc agccaggcac
    13441 cggcggcgcc ctgcgcgatc aggccggcgc cggcgatcag gatcggcagc ggccgccact
    13501 ggtagaaggt caggtcgtag aacagcagca gcgtgccgat gaccagcccc ggcagcgccc
    13561 gccagccgaa gcagagcagc ccggcgagca cgacgccggc cggcggccag aacggcagcg
    13621 gcagcccgcc cggcatcggc acgcgcgccg cgatccagcc gccggcgagc accagcacgg
    13681 caaggatcag gttgtcgatc cagaggtgtt cgccgtcctg cccggcggcg gcagggctca
    13741 tcggcagcgc gttcacgatt cggcctcgta ctgataggcg agcgcgtcga gcgtcgtcga
    13801 gttgaactcg cgccggtaca gcaccgcgac cgtcagcacc gagaccggcg cgaacagcag
    13861 cggatggatc agccagctca tcaccgcgag gccgaagtag tacgcgcgca ggccctggtt
    13921 gaactggtca ccggcgagcg agttgagccg cgcgagccct tcggcgcagc gatcgaggct
    13981 ggcacggtcc gggaagtccc cgggcatcgg tgccgccccc atcatgatcg agcagtagtt
    14041 gaactggcgc agcgaccacg tgaacttgaa gaacgcgtac acgaacagca cgccgagcac
    14101 gacgagcttg atctgccaga gctcgcggct ggcgtgtgcg acgaagggca gatcggcggt
    14161 cgcggcgacg gcgcggtcgg cggagccgaa catcgcgaac acgccgccga tgacgatgat
    14221 cgtggtcgag gcgaagaacg acacgctgcg catcaggttg ccgagcagcg tcgaatcgaa
    14281 gatgcggttg tcgcgttcga gcatgcgctc catccacacg cggcggtgcc ggtaggttgc
    14341 ctgcagcagg ctgcgccggc tcgccctgcg ctggccggcg agcacaacat agccgccgca
    14401 gcagaggctg aagcaggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    14521 cagcgcgccg ccgatcagca gcatccacca gccggagttc atgccggagc acggcagagg
    14581 ttcatcgcgg gtctccctgc aggcgcgcga ggatgtcggc acgggcttcg gcgagcaggg
    14641 aatcgcggtc gagactcgcg aggatttcgc gcacgacgcg cttcggcccc ccctcgcccc
    14701 aggaacggcc ctgcgcgaaa taccgctgcg cagcaagacc gaccacgtag gttccatacc
    14761 acgcgaccgc gccctgggct cccgccgtca cgatcgttga cagcccgaag gtcgcgccct
    14821 tcagcgccga ggacgcgagg ctcgcgaggt agctcgaccc catcagcagc gcgagctgcg
    14881 cgatcagcgt gcgcagcagc gccccggctt cggtgtgcga gaccggcaga ccgtagacgc
    14941 ggccgaggtg cacgatcatc gccacgtcgg cgaacaccgc cgcgagatcg gcgagcggca
    15001 ccgggttcag cgccacgccg agccccttgg ccaggcagta gcggcggatg acggtctcgg
    15061 cgagcgagcg gcgcagctcg acgacctcgc gcgccagccg ctcgcagaag cgcccggcga
    15121 acagcccggc gttcagcgcc gcgaccgtct tgccctcggc ggcgaggatg tcccagagcg
    15181 tctcgcgcag atcctcgatg tccggcgccg ggcgctcgcg cgattcgtgc tcgcgaccgc
    15241 ccgcgtcgac gcggatcacc gtgcgctcgg ccggggccgc ggcgcagggc acgatgtgct
    15301 cggggcgcac gaggccgcgg ctgcgggttt ccagggcttc gagcagcagc gcgcgctcgg
    15361 cacgcgtgta gcggtcggtc ttgttcagcg ccagcacgat cgggcgcgtg ccctcggcgg
    15421 cgagttcgcg cagcgcgttg agctcggtcg cggtcaggtc gccctcgcag acgaacagga
    15481 tcaggtcggc gccgccggcg acctcgcgtg ccagccgctc gcgcgcctcg ccgccgacct
    15541 cgtcgatgcc gggcgtgtcg atcaggaaca cgccgccatc ctccagccgc tgcacgccgg
    15601 gcagctccgg atcggccgcc cagcgcgccg agcttgcggc cgtggtctcg ccgtgcagcg
    15661 gactggtcgc gaagcgctgc tcgccgagca gggcgttcag cagcgccgac ttgccgacgc
    15721 tgacccggcc gaacacggcg atgtgcagat ggccgtgttc gaggcgttcg agcagcagcg
    15781 acagcgccga gtaatcctcg gccaggcgct cgcgcacggc cgccggcacg gcctcgtcgg
    15841 cgagcagcgc gcgcaggctg tcgcgcgcga ggccgaggtg cgtgtcggcc gcggacgcgg
    15901 aagggccggc catcccggga gcgccgggct caggcgtcgc tgcggtcggc gtcgcggccg
    15961 ggttcagccg atcgagcacg gccgaccacc agtgcggcca gccgtcgcgc gcgcgtttcc
    16021 agatgttcac cgagggcatc ctcgaacagg tgtaccgcca cgcccggcac cagcgcaccg
    16081 cggctcgcga gcgagcgggc ggcggcgtga cccaggctct cgaagatcag accgtaggcg
    16141 atcgcatgca gtgcgccgcc gagcacggtg ccgacgccgg gaaaggcctt cagcacgttg
    16201 ccggccagcg ccagcagcag gttcatgcgc cgcccgacgt ggcggctggc gagatcgagg
    16261 aagcgcttca ggtcgatctc ctgtgccggc acaccgtaga cggccgaaag ctccttcagc
    16321 atctgcatgc cgagccagcc ctggatcagc acgtctgtgc ccggcccgac cgcggccagc
    16381 gcgccgaaca cggccttgcg cgcgtaggtc tcgacgacgg tctcggcgcg cgcggcgcgc
    16441 tcggcggcaa gggctgcgtc gagttcgcgc ccgatcagcg tgaacaccgc ggcatcgcgc
    16501 agggattcga gcgcaccggc atcggcatcg agcgtgcgct gcagcgccac gccgagcgcg
    16561 tgcacgtcgg ccgggcgctc acgctcgatg cgcgtctcgc tgccgtcggc gccgatgcgc
    16621 acgacttcct cggtaccacc ggcactgacc gcgacgagct cggcccgtcc ggccagccgc
    16681 tcgcggatgc gcgcctgcac cagcgcgagt tccgcggcgg tgtagcgatc ggccttgttc
    16741 agcgccacca gcagcggctt gcccagactc agcaggcgct cgagcgcggc gagttcggcg
    16801 cgcgcgagat cgccgccggt cacgtagatc accgcgtgcg cacgcacggc ctcgccctcg
    16861 gcctcggcgt cgagcgtgcc gtcggcctcg ttgaggccgg gcacgtcggc gagcacgagt
    16921 tcgtccccgg ccgggctggt ccagacgtga tggacgatcg tgcgcgtgct gccgccgacc
    16981 ggcgagacca cgacctcggc gcccggcagc agcgcgcgga tcaggctgga cttgccggcc
    17041 gagacctcgc cgaaacaggc gacgatcacg cgcccgcccg cgcgccggcg ttcgagttcg
    17101 gcgagctcgg cgcggtagcg ctcgaccggc agcccggcct gcacggcagc ttcgagctgg
    17161 cgatcgatct gcggggcatc gggaggcggc ggcgcggcgg ctgcgaccgg ccggcgcggg
    17221 cgcatcaggc gcagcaccag ccacgcgacc agcgcgatca gcagcccgta cagcgtcagg
    17281 aacagcgggg atgcctccga caggcgctgc cacagcgtca gcgcgaagtc ggtgacgtgc
    17341 agcgccagcg ccagggtcag cgccagcccg agcgcgaaca gcagcggtcg ccagcgacgc
    17401 atgcgcgact caggccccgg cggcggcgca gagcgccgag cggaattcct ccggcatcgc
    17461 gcgcacgcgc cgtgtcgcgt agtcgaagaa caggatgccg gtcttggcgt gcgcgatgtc
    17521 gcggccatcg ccagcggcac tgacgcggta gacgaggtcg cagcccttgc cgtggacatc
    17581 ggcggtggcg agatcgaaca gcagcgtctc gccgaggaag gcctcggcgc ggtagacgat
    17641 gacggaatcg accatgatca gccccgcccc ccagcagtcg agttcgctct ggccgtgcgc
    17701 ggccagccag cgcacgcgcg cctcgtgcag caggccgagc agcgcgtcgt tgccgaggtg
    17761 gcggccgtag ttgatgtcac cgacgcgcac ctgcatcgag gtgcggaagg cgaaggtcgc
    17821 gggcaggtcg agcttgacgc gggccatgcg gtcacctcgg ggcgcggtgg atgaagcgtg
    17881 gacggggggg cggaaatgcg aacgccgggc ggcttgcgac cgcccggcgt ccgggacggg
    17941 aaacgacggc gctcaggcgg tgcaggccgc gaacaggcgc tcctccagcg cggtgcgcgc
    18001 ggcgagcgtg gcaccgaggc gcgcgagatc ggcggccagg cgctcgaagc tgcaggtgtg
    18061 ctcgccgccg tagcggtcgt tgaaatcgac ggccgcctgc gcggtgtcgg cgagggccgc
    18121 ctcgatctct tcgagcagcg cccgcgtgct cgcgcaggcc ggcaggccgc ggcccgggca
    18181 gctgcgcatg agctgataga cctcgaagtg cccgagcgcg acgtagtcga cgagctgctg
    18241 gcacagggct tcgagctgcg gcagcagttc ggcgatgccg agctgggcgc agccgcagga
    18301 cagggtctcg tggcgatcgg ccagggcctg gcgttccaga cgcagacgct cgaccagctt
    18361 cggcagggcc ttgtcgtgaa tctcgtcagc gatggtcgtc atctcgtcat ccagcggttc
    18421 ggcgagcatg tctcctcctc agttatgtct ggttgtcggc atgcgcgtct ctgcggacgc
    18481 tcgggcagac ggaccgcagg ctgcaacggt cgccggcatg ctaacgccgg ccgtggcgcg
    18541 ggcgccaccg gcgcgtggcc tgcggttcag ccccggacgg cagacttctt cgccacctcg
    18601 tcgcgcagat agaccggctg cgcggcagcc gccgacacgc cggcaccggc caggagcagg
    18661 cgcctgccga gccgggcgac atcggcggcg tgcggcagcg cggccggatc acggtcactg
    18721 aaggcgatgc gcccgtcgaa cgcggcagcc gccgccgcga gaccgctgcc gaccccgaac
    18781 caggtgccgg gcaggctcag cgccgaggct gccgtgacgc attcggcgag ccggggctcc
    18841 tgcggccagc catcggcatc gagcggccag gctgcggcgt agatctctcc catgcgcgca
    18901 tcgatcgccg cgaacacctg acgcgcgccc tgcctgcgac cggcgccgta cgcgagggtt
    18961 tcgagcgtcg agaccggcgc caccggcaaa tcgtgcgcga aggccagccc ctgtgcgcag
    19021 gcggtggcga tgcgcacgcc gacgaaggcg cccggcccgc gcccgaaggc gatgccatcg
    19081 agcgccgacc a
//
LOCUS       DAOQIE010000027        18930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1319_length_18930_cov_7.636874, whole genome shotgun sequence.
ACCESSION   DAOQIE010000027 DAOQIE010000000
VERSION     DAOQIE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_18930_cov_7.636874"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="ilvA"
                     /locus_tag="PK144_03165"
     CDS             <1..1407
                     /gene="ilvA"
                     /locus_tag="PK144_03165"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009495111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNF64755.1"
                     /translation="KREDLQPVFSFKLRGAYNKIAHLSPAQLERGIICASAGNHAQGV
                     ALSAQKLAIKATIVMPGTTPPIKVNACKARGARVVLHGDTFDEAAQHARQLEAEKGYT
                     FVHPYDDPDVIAGQGTIGMEILRQHPDPIHAIFVAVGGGGLIAGIATYVKFLRPEVRI
                     IGVEPEDAACLDAAMKAGERVVLDQVGIFADGVAVRQIGEETFRLCRELVDEVITVST
                     DEICAAMKDIFDDTRAIAEPAGAVGTAGLKKYVARTGIHGQNLIAINCGANVNFDRLR
                     HVAERAEIGEQREALLAVTIPERAGAFRHFIQSLGRRAITEFNYRYADARDAQIFVGV
                     QLTEGREEKLALIEQLRTEGYPVLDLSDNEMAKLHVRYMVGGHAPGIADERLYRFQFP
                     ERPGALLKFLNSMVHGWNISLFHYRNHGADYGRVLAGIQVPDTDLAQFRQALLDLGYD
                     HWEETGNPAYRLFLDGGR"
     gene            complement(1603..3090)
                     /gene="ppx"
                     /locus_tag="PK144_03170"
     CDS             complement(1603..3090)
                     /gene="ppx"
                     /locus_tag="PK144_03170"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNF64756.1"
                     /translation="MPVDTFAAIDLGSNSFHMIVARAGDGTLQVVDRLREMVQLAAGL
                     DAAHRLDEATQQRALDCLARFGQRIAQLPPERVRIVGTNTLRQARNGAAFLERATQVL
                     GHRVEIISGREEARLIYLGVAHSTAGSGRRLVVDIGGGSTELIVGEQFEPQQLESLYM
                     GCVSLTRRFFDAERIRERALHEADLSVRMELEPVEAGFRDAAWDEAIGASGTIKAIRD
                     VLVALGWSSGDIPREALRRLRADLIDAAPAVFGERFGLARERAAVFAGGLAVLHGIFE
                     TLGIECMKVSDGALREGLIFDLLGRVQHEDVRDRSVRALAARFGCDEAQGGRVAATAR
                     ELLAQAAPGWGIADEEAARYLDWSARLHELGLAIAHNQYHKHGGYVLASADLPGFSKT
                     EQALLAALVRGHRRKLPLDTFTALPAGQAERGLRLCLLLRLACVLHRARSRQPLPALH
                     LQPGERELRLGFPAGWLDEHPLTRADLAAEADYLATARCRLVIDE"
     gene            complement(3139..3636)
                     /locus_tag="PK144_03175"
     CDS             complement(3139..3636)
                     /locus_tag="PK144_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF64757.1"
                     /translation="MQLLLIRHGIAEDTAPSGGGDAERALTDDGARRLRKGANRLRMQ
                     FERLDHVLCSPYRRTRESAAILCAAWGLAAPQTIAGCTPHDAPEDVLPVLAGLDPDAH
                     VALVGHEPHLGCLAGLLLSGSAEPLLRLRKGGAALIVFPGAPAAGTGRLHWALTAGQM
                     RALRD"
     gene            complement(3798..5111)
                     /gene="phoR"
                     /locus_tag="PK144_03180"
     CDS             complement(3798..5111)
                     /gene="phoR"
                     /locus_tag="PK144_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003154443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HNF64758.1"
                     /translation="MQSPWPGALWRTLLILAAAAGLGWQAGHPAPGLALGATLLCGWH
                     LWRLSRLERWLRDGCRRRRPQPDGIWGEIYYHLQRRRQRRRDDTRRYIDTLRQFQEAA
                     RAMPDATVVLDDADTVLWSNPAAEDLLGLRSPQDLGTRITYLVRHPSFVAFLGRRDAQ
                     AVCDFPSPVGGQPLSVRAIPYGEANRLLIASDVSRVHRLEQVRRDFVANVSHELRTPL
                     TVIGGYLETLLDSGDACAVKWQQPLRSMQHQSARMLHIIEDLLMLAKLEGQQERLPPR
                     PVAVPGMLAAIAEDAIALSGEQQHTVRVDADPALWLCGHEQELRSAFSNLVFNAIRHT
                     PAGRHVDIRWHADARGVHLEVIDNGDGIAPHHLPRLTERFYRVDRGRNRERGGTGLGL
                     AIVKHVMSRHGGQLRIASTVGAGSRFACDFPAELACPRTSGGRAA"
     gene            complement(5126..5818)
                     /gene="phoB"
                     /locus_tag="PK144_03185"
     CDS             complement(5126..5818)
                     /gene="phoB"
                     /locus_tag="PK144_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNF64759.1"
                     /translation="MSEKRILIVEDEQPIREMITFALGAAGYTTVEAADARAALALIA
                     ERVPDLILLDWMLPGASGLELARRLKKDELTRDVPVIMLTARAEEEDKIHGLESGADD
                     YVTKPFSPRELAARIKAVLRRGSGQGSDEAFEADGLRLEPLSHRVTAREQTVDIGPTE
                     YRLLEFFMSHPERVYSRSQLLDRVWGSNVYVEERTVDVHIRRLRKVLEPSGHDVLIQT
                     VRGTGYRFSTRT"
     gene            6108..6530
                     /gene="dksA"
                     /locus_tag="PK144_03190"
     CDS             6108..6530
                     /gene="dksA"
                     /locus_tag="PK144_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNF64760.1"
                     /translation="MSAESFTGFTPYQAKPDEEYMNDAQKAHFRSILLSWKQELMEGS
                     QRTVTHMQDEPLNLPDPNDRASQEEEFSLELRARDRERKLLRKIEKSLQRMAEDEYGY
                     CEGCGIEIGLRRLEARPTAELCIECKQVEEIKEKSRAA"
     gene            complement(6588..8876)
                     /gene="ptsP"
                     /locus_tag="PK144_03195"
     CDS             complement(6588..8876)
                     /gene="ptsP"
                     /locus_tag="PK144_03195"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNF64761.1"
                     /translation="MLDSLQRIVQEVSSAHDLDEALALIVERVKASMNADVCSVYLSD
                     EARREYVLMATDGLRPGAVGRVRIPFGKGVVGLCASRAEPVNLENAPDHPAFIFIAST
                     GEAPFHGFLGVPIVRRRKVMGVLVVQQREQRRYIDEEVAFLVTLAAQLAGSITQAELR
                     QSLDQMDVDDLDTLFVDGVACARGLGIGRALVLFPEARLDSVPDKKLGLDLDLIEAEC
                     SRFRLAVDAELEELKSLAARMGRILSPGDQALFDAYAMLLGSDSLVNGTLERIHAGNW
                     APGALRATVAEYANVFEEMDDAYLRERAADIRDLGGRLLRRLHQNKDAPPVEYPERCV
                     LVGEELNVSQLLDVPPERLVGLVSVRGTGASHVALLARGLGIPAVFGVTGAPLTKLNG
                     RDVVVDGYSARICIQPGTQLREEYERLAREEAELSRGLLELREEPAITPDGQRIHLLA
                     NSGLFADINAARDSGAEGIGLYRSEIHFFLRDRFPGEDEQTATYARVLTSMAPRPVTL
                     RTLDVGGDKPLPYFPIREDNPFLGWRGIRISLDHPDIFKTQLRAMLRAGAATGSTDGL
                     SVLFPMITMVSELDEALELLRQAHAELVEDGVPAHFPKVGVMIEVPAAAYQVELLAKR
                     VDFVSIGSNDLTQYLLAVDRNNDRVAKLYDSLNPAVIGVMQWLVERARRAGKPVSVCG
                     EMAGDPAAALLLIGMGIDSLSMSLGNLLKVKWMIRSVPLSVAQALVADVLGLESAQQI
                     RAAAEEVMIAHGLAGLVRPGQH"
     gene            complement(8943..9569)
                     /locus_tag="PK144_03200"
     CDS             complement(8943..9569)
                     /locus_tag="PK144_03200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNF64762.1"
                     /translation="MCDPLVNGLQVIDSEGFRANVGIILCNTEGRLFWGRRIGQKSWQ
                     FPQGGIRRHETPEEAMLRELGEETGLRPEHVEILGRTEGWLRYRLPKRLIRRHSKPTC
                     IGQKQVWFLLRLRGCEKDFCLDGSERPEFDHWVWVDWWYPLEAVVAFKRQVYLRALHE
                     LLRFAEQPQLPREPSPAVLARFNLTLQALRRAPAAVLPVRSEPNVRPH"
     gene            9816..10472
                     /locus_tag="PK144_03205"
     CDS             9816..10472
                     /locus_tag="PK144_03205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF64763.1"
                     /translation="MSLALFDLDHTLLAGDSGSLWGRHLCAVGALDAAHFEAENRRFG
                     AVYIAGTLDPLAYFTFAMAPLAAHPIARLLEWREQFIEQHIRPVVLARAVELLDAHRA
                     RGDTLVIVTATDRFVTEPIAALLGVPHLLATEPEQLDGGYTGRVAGEPCFREGKIAAL
                     ERWLGGHGARLDEAWFYSDSQNDLPLLGRVGHPVAVDPDPVLAAEAAARGWPVLSLRD
                     "
     gene            10486..11460
                     /locus_tag="PK144_03210"
     CDS             10486..11460
                     /locus_tag="PK144_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNF64764.1"
                     /translation="MNRSPLRICAALVLAALAAFALAAASGSTPIPLARLPAALAGDG
                     PAALIVRELRLPRAFAAFVTGGLLALAGALMQVLLRNPLADPYVLGLSGGAAVGALTM
                     LLAGLAGVWVDAGAAAGALAAVVLVFGLAHGREAWSPLRLLLTGVVTAAGWSALIALL
                     LALAPDRALRGMVFWLLGDLSAAARLAPAALTLAFGLGAALLLAPRLNVLMHGELAAA
                     SLGVAVRPLRLALYALASLCAAAAVTVAGPVGFVGLVVPHGVRLAAGSDHRLLLPASA
                     LAGGTLLVLADTLARTAIAPQQLPLGAVTALVGVPAFLWLLNRGRHGG"
     gene            11450..12253
                     /locus_tag="PK144_03215"
     CDS             11450..12253
                     /locus_tag="PK144_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64765.1"
                     /translation="MAADAGTATAPAPAPLLEARGLSVEVGTHRVCTGLGFALRAGER
                     WGLLGANGSGKTTLLHTLAGLRAPQAGEVRLDGLPLTALAPRRRALRLGLLPQEHEDA
                     FPATVAETALTGRFPHLGRFGHAGAADRAAVEAALAATGLAAHAQRQVATLSGGERRR
                     LGFATLLAQDPAVLLLDEPTNHLDLGYQVRLLDALAARVAAEGRGWIMVSHDATLVAR
                     FCTHVLLVYGDGRTATGGPQLLCPQQLSALYGHPVLRLDGPLGPVFAPG"
     gene            12322..13170
                     /locus_tag="PK144_03220"
     CDS             12322..13170
                     /locus_tag="PK144_03220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNF64766.1"
                     /translation="MTLPPEDRSRRQFLSALAAAGFAAAVQPVCADTILTPTDGLDAG
                     MDRVAANDGFLPVYRAVPQGRKNLPLILVVQEIFGLHEHIKDICRRLARLGYCAIAPD
                     LYYRQGDATKVSDIPTLLKTIVAFVPDEQVLRDLDTVVRWAQGSGYYDGHRLGITGFC
                     WGGRITWLYCAHNPQVRAGVAWYGKLEGEETAMTPQHPINIADSLQVPVLGLYGGQDA
                     GIPQDSLGRMRSRLASAHSQSEIVIYPDAPHAFNADYRPSYRKAAAEDGWRRMREWFS
                     VHGVNV"
     gene            13340..13474
                     /locus_tag="PK144_03225"
     CDS             13340..13474
                     /locus_tag="PK144_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNF64767.1"
                     /translation="MDTLKLLFSDWTGILSALVILFMLGMGVYFGWLFIGSSGDGPKS
                     "
     gene            14578..15795
                     /locus_tag="PK144_03230"
     CDS             14578..15795
                     /locus_tag="PK144_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF64768.1"
                     /translation="MPQSRKSEWPLILGLVLPLSLVAVTLSGCDEPQAAVKPAAPAPV
                     EVGVVEIRPEPLTLTTELPGRTRAHLVAEVRPQVGGIIEQRLFREGAEVGAGDLLYRI
                     DPASFQAAYDSAKAALAKAQADIHSVQLKAERYKDLVRIKAVSDQDYDDARAALAQAQ
                     AEVEAQKAALETARINLDYTQVRAPIAGRIGRSSVTPGALVTADQTTALATIQQLDPV
                     YVDVQRSSTEWLELQQALKSGRLSHPGADGSITVRLKLEDGSDYPQTGRLLFSEVTVD
                     AGTGAITLRAEFPNPDQRLLPGMYVRAVLEEGIAQQALLVPQRAVSRDARGKASALVV
                     NADGKAERRDLVTERAVGDRWLVTTGIAAGERVIVDNLQKVKAGALVKPLPWSAPKRE
                     TAGLRPLTDLDPI"
     gene            15812..>18930
                     /locus_tag="PK144_03235"
     CDS             15812..>18930
                     /locus_tag="PK144_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF64769.1"
                     /translation="MARFFIDRPIFAWVISITIMLAGALAIFTLPIAQYPDIAPPTVA
                     INATYPGASAKTIEDSVTQVIEQNMKGIDGLQYMSSNSSSAGTARVELTFSADTNPDI
                     AQVQVQNKLQLALALLPQAVQSQGVTVTKSANSFLAVYAFVSDRDDVTGTDIADFVSN
                     QVLDPISRIDGVGETTLLGARYAMRIWLDPAKLVKYALTPADVKSAIEAQNTQVAVGQ
                     LGDLPAAPGTGLNATINARSRLQTPEQFRAIVLRSATDGATVRLSDVARVEVGAESYS
                     VLARFNGRPAAGLAIRLASGANALAVAEAAEAKIRELERFFPSGIRAVIANDTSPFVR
                     LSIEEVVKTLIEAVVLVFGVMYLFLQSWRATLIPTIAVPVVLLGTFGVLAALGYSINT
                     LTLFAMVLAIGLLVDDAIVVVENVERMMAEQKLPPLEATRRSMDQISGALIGIALVLS
                     AVFVPMAFIQSSSGVIYRQFSVTIVSAMALSVLVALTLSPALCATLLRPLPAGHHAGG
                     HGRLGRAFAAFNRGFGRTTDRYAAAAGGIVRRPLRFVLLYVLLLGVLGVLFLRLPTSF
                     LPDEDQGTLYAQITLPVGATQERALDVIAQVENYFLDHEKDAVRSVFAVSGFGFGGNG
                     QNVATAFIKLRDWSLRDRDELRAPAIVERATKTLGRIRDAQVFVMNPPQIRGLGNSTG
                     FDVQMQDSGGVGHEALVAARERFLELARQEPLLTRVRPQGLDDTPQLQVDIDTAKAGA
                     LGLTMSEVNDALGTALGGDYVNDYLDRGRVKKVYVQGDAPFRMRPEDISRWYVRNGDG
                     AMVPFSDFSHAYWVYGSPRLERFNGSSSIEIVGEAAPGRSSGEAMAAVERIMGQMPQG
                     IAYEWTGLSRQERQSGSQAPYLYAVSVLVVFLALAALYESWSIPFAVLLVVPLGVIGA
                     VTATELRGLENDVYFQVGLLTTIGLSAKNAILIVEFAHELQRKGMGLIEATLSAAHQR
                     LRPIVMTSAAFMLGVLPLAISTGAGSGSRHSIGTGVLGGMLSATVLALFFVPLFFVLV
                     RRYLGGRERA"
BASE COUNT         2732 a         6642 c         6706 g         2850 t
ORIGIN      
        1 aagcgcgagg acctgcagcc ggtgttctcg ttcaagctgc gcggcgccta caacaagatc
       61 gcgcacctga gcccggccca gctcgaacgc ggcatcatct gcgcatcggc cggcaaccac
      121 gcgcagggcg tggcgctgtc ggcgcagaag ctggcgatca aggcgacgat cgtgatgccg
      181 ggcacgacgc cgccgatcaa ggtcaacgcc tgcaaggcac gcggcgcgcg cgtggtgctg
      241 cacggcgaca ccttcgacga ggccgcgcag cacgcccgcc agctcgaagc cgagaagggc
      301 tacaccttcg tgcacccgta cgacgacccg gacgtgatcg ccggtcaggg cacgatcggc
      361 atggagatcc tgcgccagca cccggacccg atccacgcga tcttcgtcgc ggtcggcggc
      421 ggcgggctga ttgccggcat cgccacctac gtgaagttcc tgcgcccgga ggtccgcatc
      481 atcggcgtcg agcccgagga tgccgcctgc ctcgacgcgg cgatgaaggc cggcgagcgc
      541 gtcgtgctcg atcaggtcgg catctttgcc gacggcgtgg cggtgcgtca gatcggcgag
      601 gagaccttcc gcctctgccg cgagctggtc gatgaagtga tcacggtcag caccgacgag
      661 atctgcgcgg cgatgaagga catcttcgac gacacgcgcg cgatcgccga accggccggc
      721 gcggtcggca ccgcgggcct gaagaagtac gtcgcgcgca ccggcatcca cggccagaac
      781 ctgatcgcga tcaactgcgg tgccaacgtc aacttcgacc gcctgcgcca cgttgccgag
      841 cgcgccgaga tcggcgagca gcgcgaggcg ctgctggcgg tgaccatccc cgagcgcgcc
      901 ggcgccttcc gccacttcat ccagtcgctc ggtcggcgcg cgatcaccga gttcaactac
      961 cgctacgccg atgcgcgtga tgcgcagatc ttcgtcggcg tgcagctcac cgagggccgc
     1021 gaggaaaagc tcgcgctgat cgaacagctg cgcaccgagg gctatccggt gctcgacctg
     1081 agcgacaacg agatggccaa gctgcacgtg cgctacatgg tcggcggcca cgcgcccggc
     1141 atcgccgatg agcgcctgta ccgcttccag ttcccggagc ggcccggcgc gctgctgaag
     1201 ttcctgaact cgatggtgca cggctggaac atctcgctgt tccactaccg caaccacggc
     1261 gccgactacg gccgcgtgct ggccggcatc caggtgccgg acaccgatct cgcgcagttc
     1321 cgccaggccc tgctcgacct cggctacgac cactgggaag aaaccggcaa cccggcctac
     1381 cggctgttcc tcgacggcgg ccgctgaggc cggcgcacac cagccctccg gaccgtgatg
     1441 ggtccggagg acttctgcat tcgggccggc tgactcagcc gtgcggaccc ggagaggatt
     1501 gccaaaccat ctggttcatg ccggtcacgg cagaacgcct gtcttttcag gttcttgccc
     1561 atcgcgggag gagcggggac cgcaggctcc ggcaagcttt cctcactcgt cgatgaccag
     1621 ccggcagcgg gccgtggcca gatagtcggc ctcggcagcc agatccgcgc gggtcagcgg
     1681 gtgctcgtcg agccagccgg ccgggaagcc gagccgcagt tcgcgctcgc ccggctgcag
     1741 atgcagcgcc ggcagcggct ggcggctgcg ggcgcggtgc agcacgcagg ccagacgcag
     1801 cagcaggcag aggcgcaggc cgcgttcggc ctgaccggcc ggcagcgccg tgaaggtgtc
     1861 gagcggcagc ttgcggcggt ggccgcgcac cagcgccgcg agcagggcct gctcggtctt
     1921 cgagaagccc ggcaggtcgg cgctggcgag cacgtagccg ccgtgcttgt ggtactggtt
     1981 gtgcgcgatc gccagcccga gttcgtgcag gcgcgcggac cagtccagat agcgtgccgc
     2041 ttcctcatcg gcgatgcccc agccgggcgc ggcctgcgcg agcagttcgc gggcggtggc
     2101 agcgacgcgg ccgccctggg cctcgtcgca gccgaagcgg gcggcgaggg cgcgcacgct
     2161 gcggtcacgc acgtcctcgt gctgcacacg cccgagcagg tcgaagatca ggccctcgcg
     2221 caaggcgcca tccgatacct tcatgcattc gatccccagc gtctcgaaga tgccgtggag
     2281 cacggccagc ccgccggcga acaccgcggc gcgttcgcgt gccagcccga agcgctcgcc
     2341 gaacacggcc ggcgcggcgt cgatcagatc ggcgcgcagg cgacgcagcg cctcgcgcgg
     2401 gatgtcgccg ctgctccagc cgagcgcgac cagcacgtcg cggatggcct tgatcgtgcc
     2461 ggaggcgccg atggcctcgt cccaggcggc gtcgcgaaag cccgcctcga ccggttccag
     2521 ttccatgcgc acgctgaggt cggcttcgtg cagcgcgcgc tcgcggatgc gttcggcatc
     2581 gaagaagcgg cgcgtcaggc tcacgcagcc catgtacagg ctttcgagct gctgcggctc
     2641 gaactgttcg ccgacgatca gctcggtgct gccgccgccg atgtcgacca ccagccgccg
     2701 gccgctgccg gcggtcgagt gcgcgacgcc gaggtagatc agccgcgctt cctcgcgacc
     2761 gctgatgatc tcgacgcggt ggccgagcac ctgcgtggcg cgctccagaa aggccgcgcc
     2821 gttgcgcgcc tgacgcagcg tgttggtgcc gacgatgcgc acgcgctcgg gcggcagctg
     2881 cgcgatgcgc tggccgaagc gggcgagaca gtcgagcgcg cgctgctgcg tggcctcgtc
     2941 gaggcggtgc gcggcgtcga ggccggcggc gagctggacc atctcgcgca ggcgatcgac
     3001 gacctgcagc gtgccgtcgc cggcgcgggc gacgatcatg tggaagctgt tggagccgag
     3061 gtcgatcgcg gcgaaggtgt cgacgggcat gcggacgatc cggcggcggg cggggaggcg
     3121 gacagtgtaa ggcgggcttc agtcgcgcag cgcgcgcatc tggccggcgg tcagggccca
     3181 gtgcagccgg ccggtgccgg cagccggcgc accgggaaac acgatcagcg ccgcgccgcc
     3241 cttgcgcaac cgcagcagcg gttcggcgct gccgctgagc agcagcccgg cgaggcagcc
     3301 gaggtgcggc tcgtggccga ccagcgccac gtgcgcatcc ggatcgagtc cggcgagcac
     3361 cggcagcacg tcctcgggcg cgtcgtgcgg ggtgcagccg gcaatggtct gcggtgcggc
     3421 cagcccccag gccgcgcaca ggatcgcggc gctctcgcgc gtccgccggt acgggctgca
     3481 gagcacgtgg tccagccgtt cgaactgcat gcgcaggcgg ttggcgccct tgcgcaggcg
     3541 ccgggcgccg tcgtcggtca gcgcacgctc ggcatcgccg cccccggacg gggcggtgtc
     3601 ctcggcgatg ccgtggcgga tcagcagcag ttgcatggcg gattcctccc taatgaaggc
     3661 ggttcgcggc ggggaagtcg ggccgatggt acgggcatga cttttctccc ctcttgccag
     3721 tcccttgccc gcgaggggcg aggggctgaa agacgctgaa agacgctgaa agaaaaaacg
     3781 ttccggcgtg accggcctca ggcggcgcgg ccgccgctgg tgcgcgggca ggccagttcg
     3841 gccgggaaat cgcacgcgaa gcggctgccg gcgccgacgg tgctggcgat gcgcagctgg
     3901 ccgccgtggc ggctcatcac gtgcttgacg attgcaagcc cgaggccggt gccgccgcgc
     3961 tcgcggttgc ggccgcgatc gacccgatag aagcgttcgg tcagccgcgg caggtggtgc
     4021 ggtgcgatgc cgtcgccatt gtcgatgact tcgagatgca cgccgcgtgc gtcggcatgc
     4081 cagcggatgt cgacgtggcg gccggccgga gtatggcgga tggcgttgaa cacgaggttc
     4141 gagaaggcgc tgcgcagctc ctgttcgtgg ccgcagagcc acagcgccgg atcggcgtcg
     4201 acgcgcaccg tgtgctgctg ctcgccggac agcgcgatgg cgtcctcggc gatcgccgcg
     4261 agcatgccgg gcacggcgac cgggcgcggc ggcaggcgct cctgctggcc ttcgagcttc
     4321 gcgagcatca gcaggtcctc gatgatgtgc agcatgcgcg cgctctggtg ctgcatgctg
     4381 cgcagcggct gctgccattt gacggcgcag gcgtccccgc tgtcgagcag ggtttcgagg
     4441 tagccgccga tcacggtcag cggcgtgcgc agctcgtgcg agacgttggc gacgaagtca
     4501 cggcgcacct gttcgagccg gtgcacgcgc gagacatcgc tcgcgatcag cagccggttg
     4561 gcctcgccgt aggggatcgc gcgcaccgac agcggctggc cgccgaccgg cgacgggaag
     4621 tcacagaccg cctgcgcgtc gcgccggccg aggaaggcga cgaagctcgg gtggcgcacc
     4681 agataggtga tgcgcgtgcc gaggtcctgc ggcgagcgca ggccgagcag gtcctcggcg
     4741 gccgggttgc tccagagcac ggtgtcggcg tcgtcgagca ccacggtggc atcgggcatg
     4801 gcgcgggcag cctcctggaa ctggcgcaag gtgtcgatgt agcggcgcgt gtcgtcacgc
     4861 cggcgctgcc ggcggcgctg caggtggtag tagatctcgc cccagatgcc gtcgggctgc
     4921 ggccggcgcc ggcggcagcc gtcgcgcagc cagcgctcca gccggctcag ccgccacaga
     4981 tgccagccgc agagcagcgt cgcgccgagc gcgagccccg gggcgggatg accggcctgc
     5041 cagccgagac cggcagcggc ggcgaggatc agcaacgtgc gccagagggc gccgggccac
     5101 gggctctgca attcgggccc ccgcctcagg ttctcgtcga gaaacggtag ccggtgccgc
     5161 gcacggtctg gatcagcaca tcgtggccgc tcggctccag caccttgcgc agccggcgga
     5221 tgtgcacgtc gacggtgcgc tcctcgacgt agacgttgct gccccagacg cggtcgagca
     5281 gttgcgagcg cgaatacacg cgctccgggt gcgacatgaa gaactccagc agccggtact
     5341 cggtcgggcc gatgtcgacg gtctgctcgc gggcggtgac gcggtggctc agcggttcca
     5401 ggcgcaggcc gtcggcctcg aaggcctcgt cgctgccctg accgctgccg cggcgcagca
     5461 ccgccttgat gcgcgcggcc agctcgcgcg gcgagaacgg cttggtcacg tagtcgtcgg
     5521 cgccgctttc gaggccgtgg atcttgtcct cctcctcggc gcgcgccgtc agcatgatga
     5581 ccggcacatc ccgggtcagt tcgtccttct tcagccgccg cgccagttcc aggccgctgg
     5641 caccgggcag catccagtcg agcaggatca ggtccggcac gcgctcggcg atcagggcga
     5701 gtgcggcgcg cgcatcggcg gcctcgacgg tcgtgtagcc ggcggcaccg agcgcgaagg
     5761 tgatcatctc gcggatcggc tgttcgtcct cgacgatcag gatgcgtttc tcgctcatga
     5821 cggcacgggt tctcgggctg gcggggaggg cgccggcatt aaaggcgagc aatgtgacag
     5881 gtacgtgaat ccatggcgag ccggtggcca ttgcgtgacg ccgtggtgac agtcggcaaa
     5941 tccggggact tgtccatgac aatcaggcaa gacagccact tcggccattc ccacacaccg
     6001 gaagttgtga tttcatgccg cgcgcttgcg gaccgggcct ggccggctgc gcaccgcctc
     6061 cgcccgcgcg gatggtcttg ctgccttaca tctacgggag gtcggtcatg tcagccgaga
     6121 gcttcacagg cttcacgccg tatcaggcca agccggacga ggaatacatg aacgatgcgc
     6181 agaaggcgca tttccgcagc atcctgctgt cgtggaagca ggaactgatg gaaggcagcc
     6241 agcgcaccgt cacccacatg caggacgagc cgctgaacct gccggacccg aacgaccggg
     6301 ccagccagga agaggaattc tcgctcgaac tgcgcgcgcg cgaccgcgag cgcaagctgc
     6361 tgcgcaagat cgagaaatcc ctgcagcgca tggccgagga cgaatacggc tactgcgagg
     6421 gctgcggcat cgagatcggc ctgcgccgtc tcgaagcccg tccgaccgcc gaactctgca
     6481 tcgagtgcaa gcaggtcgag gaaatcaagg aaaagtcccg cgccgcctga aggcgcgcgg
     6541 cccgcgcgcc cgcggttccg gtcctcccgc cgcagcgctg ccgtgcgtca gtgctggccc
     6601 ggacgcacca gtccggccag gccgtgcgcg atcatcacct cctcggcggc tgcgcggatc
     6661 tgctgcgcgg attcgagccc gagcacgtcg gcgaccagcg cctgcgcgac cgacagcggc
     6721 accgagcgga tcatccactt caccttcagc aggttgccga ggctcatcga caggctgtcg
     6781 atgcccatgc cgatcagcag cagcgccgcc gccgggtcgc cggccatctc gccgcagaca
     6841 ctgaccggct tgccggcgcg ccgcgcccgc tccaccagcc actgcatcac gccgatcacc
     6901 gccgggttca ggctgtcgta gagcttggcg acgcggtcgt tgttgcgatc gaccgcgagc
     6961 aggtactgcg tcaggtcgtt cgagccgatc gacacgaaat cgacgcgctt cgccagcagc
     7021 tcgacctgat acgccgccgc cggcacctcg atcatcacgc cgaccttcgg gaagtgcgcc
     7081 ggcacgccgt cctcgaccag ctcggcgtgc gcctgacgca gcagttcgag cgcctcgtcg
     7141 agttccgaca ccatcgtgat catcgggaac agcaccgaca gcccgtcggt gctgccggtg
     7201 gcggcgccgg cgcgcagcat cgcgcgcagc tgcgtcttga agatgtccgg gtgatcgagc
     7261 gagatgcgga tgccgcgcca gccgaggaag ggattgtcct cgcggatcgg gaagtacggc
     7321 agcggcttgt cgccaccgac atcgagcgtg cgcagcgtca ccgggcgcgg cgccatgctg
     7381 gtcagcacgc gcgcgtaggt cgcggtctgc tcgtcctcgc ccggaaagcg gtcgcgcagg
     7441 aagaagtgga tctccgagcg gtacaggccg atgccctcgg cgccgctgtc gcgcgcggcg
     7501 ttgatgtcgg cgaacagccc ggagttggcg agcaggtgga tgcgctggcc gtcgggcgtg
     7561 atcgccggct cctcgcgcag ttcgagcagg ccgcgcgaca gttcggcttc ctcgcgcgcc
     7621 agacgctcgt attcctcgcg cagctgtgtg ccgggctgga tgcagatgcg tgcgctgtag
     7681 ccgtcgacca ccacgtcgcg gccgttgagc ttggtcagcg gcgcgccggt gacgccgaac
     7741 accgccggaa tgccgagccc gcgcgccagc agtgcgacgt gcgaggcgcc ggtgccgcgc
     7801 accgacacca gcccgaccag acgctcgggc ggcacgtcga gcagctgcga gacgttgagt
     7861 tcctcgccga ccagcacgca gcgttccggg tactcgaccg gcggtgcgtc cttattctgg
     7921 tgcagccggc gcagcagccg gccgccgaga tcacggatgt cggcggcacg ctcgcgcagg
     7981 taggcgtcgt ccatctcctc gaacacgttc gcgtattcgg cgacggtcgc gcgcagcgcc
     8041 ccgggcgccc agttaccggc gtggatgcgc tccagcgtgc cgttcacgag gctgtcggag
     8101 ccgagcagca tcgcgtaggc atcgaacagc gcctgatcgc cgggcgacag gatgcggccc
     8161 atgcgcgcgg cgaggctctt cagttcctcc agctcggcat cgacggcgag gcgaaagcgg
     8221 ctgcactcgg cctcgatcaa gtcgaggtca aggccgagct tcttgtccgg caccgaatcg
     8281 agccgcgcct ccgggaacag caccagcgcc cggccgatgc cgaggccgcg ggcgcaggcc
     8341 acgccgtcga cgaacagcgt gtcgagatca tcgacatcca tctggtcgag cgactggcgc
     8401 agctcggcct gcgtgatcga gccggcgagc tgcgcggcga gcgtgacgag gaaggcgacc
     8461 tcctcgtcga tgtagcggcg ctgctcgcgt tgctgcacga ccagcacgcc catgaccttg
     8521 cgccggcgca cgatcggcac gccgaggaag ccgtggaacg gcgcctcgcc ggtgctggcg
     8581 atgaagatga aggccgggtg atcgggggcg ttctcgaggt tgaccggctc ggcgcggctc
     8641 gcgcacaggc cgaccacgcc cttgccgaac gggatgcgca cgcgcccgac cgcgccgggg
     8701 cgcaggccgt cggtggccat cagcacgtat tcgcgccgcg cctcgtcgct gaggtacacc
     8761 gagcagacgt cggcgttcat gctggccttg acgcgctcga cgatcagcgc cagcgcctcg
     8821 tccagatcat gcgcggagct gacctcctgc acgatgcgct gcagggaatc gagcatgggt
     8881 accggttcct cggcggaggc agacgaaagg gatgatgagg gtccggtggc gaccggaccg
     8941 cttcagtgcg gccggacgtt gggctcgctg cgcaccggca gcaccgccgc cggtgcgcgg
     9001 cgcagcgcct gcagcgtcag gttgaagcgg gcgagcaccg ccggcgacgg ttcgcgcggc
     9061 agttgcggct gctcggcgaa gcgcagcagt tcgtgcagcg cgcgcagata gacctgacgc
     9121 ttgaacgcga ccacggcttc gagcggatac caccagtcta cccagaccca gtggtcgaac
     9181 tcggggcgtt cggagccgtc gaggcagaaa tccttctcgc agccgcgcag gcgcagcagg
     9241 aaccagacct gcttctggcc gatgcaggtc ggcttcgaat gccggcggat cagccgcttc
     9301 ggcagccggt agcgcagcca gccctcggtg cgaccgagaa tctcgacatg ctccgggcgc
     9361 aggccggttt cctcgccgag ttcgcggagc atggcttctt ccggcgtttc gtgacggcgg
     9421 atgccgccct gcggaaactg ccaggatttc tgcccgatcc ggcgccccca gaacaggcgg
     9481 ccctcggtat tgcagaggat gatgccgacg ttggctctga agccttccga gtcaatcact
     9541 tggagaccgt ttacaagtgg gtcgcagatg tgtgcgttaa cggcaagggt aaaccgtggg
     9601 ttggccggaa acaatggcgt acacagccat gccggcagcg tctgtcaaga cggccgcgtg
     9661 cgccgcccga tggctgccgg atccggcagg aagccgcgaa tttccgggcg gttcgccgct
     9721 tcgccgctcc ggagggcgtg attgccgctg cgcgcggcct tccgctacgc tcgcgcacct
     9781 tcatccccgt cacccctgcc cccggaacca tcgccgtgag cctcgcgctg ttcgacctcg
     9841 atcacaccct gctcgccggc gacagcggtt cgctgtgggg gcggcacctg tgcgcggtcg
     9901 gcgccctcga tgccgcgcac ttcgaggccg agaaccgccg cttcggcgcc gtgtacatcg
     9961 ccggcacgct cgatccgctg gcctacttca cgttcgcgat ggcgccgctg gccgcgcacc
    10021 cgatcgcgcg gctgctcgaa tggcgcgaac agttcatcga gcagcacatc cggccggtcg
    10081 tgctcgcgcg cgccgtcgaa ctgctcgatg cgcaccgtgc acgcggcgac acgctggtca
    10141 tcgtcaccgc caccgaccgc ttcgtcaccg agccgatcgc ggcgctgctc ggcgtgccgc
    10201 acctgctcgc gaccgaaccc gagcagctcg acggcggcta caccggccgc gtcgccggcg
    10261 aaccgtgctt tcgcgaaggc aagatcgcgg cgctggagcg ctggctcggc gggcacgggg
    10321 cccggctcga cgaggcctgg ttctacagcg actcgcagaa cgacctgccg ctgctcgggc
    10381 gcgtcggcca tccggtcgcg gtcgacccgg acccggtgct ggcggccgag gcggcggcgc
    10441 gcggctggcc ggtgctgagc ctgcgcgact gatccgcccc gcgccttgaa ccgctcgcca
    10501 ctgcgcatct gcgccgcgct cgtgctcgcg gcgctggcgg cgttcgcgct ggcggcggcg
    10561 agcggcagca cgccgatccc gctcgcgagg ctgccggcag ccctcgccgg cgacgggccg
    10621 gcggcgctga tcgtgcgcga gctgcgcctg ccgcgggcgt tcgccgcctt cgtcaccggc
    10681 gggctgctgg cgctcgccgg ggcgctgatg caggtgctgc tgcgcaatcc gctggccgat
    10741 ccctacgtgc tcgggctgtc gggcggggcg gcggtcgggg cgctgacgat gctgctcgcc
    10801 gggcttgccg gcgtctgggt cgatgccggc gccgcggccg gcgcgctcgc ggcggtcgtg
    10861 ctggtgttcg gactcgcgca cgggcgcgag gcctggtcgc cgctgcgcct gctgctgacc
    10921 ggcgtggtca cggccgccgg ctggagcgcg ctgatcgcgc tgctgctggc gctggcgccg
    10981 gaccgggcgc tgcgcggcat ggtgttctgg ctgctcggcg acctgtcggc ggcggcgcgc
    11041 ctggccccgg cggcgctgac gctggccttc gggctcggcg cggccctgct gctggcgccg
    11101 cggctgaacg tgctgatgca cggggaactc gccgcggcga gcctcggcgt cgcggtgcgg
    11161 ccgctgcgcc ttgcactgta cgcgctggcc tcgctgtgcg cggcggcggc ggtgaccgtc
    11221 gccgggccgg tcggcttcgt cggcctggtc gtgccgcacg gcgtgcgcct cgcggccggc
    11281 tccgaccatc gcctgctgct gccggcgagc gcgctggccg gcggcacgct gctggtgctc
    11341 gccgacacgc tggcgcgcac cgcgatcgcc ccgcagcagc tgccgctcgg cgcggtcacg
    11401 gcgctggtcg gcgtgccggc cttcctgtgg ctgctgaacc ggggccggca tggcggctga
    11461 cgcaggaact gcaactgccc cggctcctgc cccgctgctc gaagcgcgcg ggctgagcgt
    11521 cgaggtcggc acgcaccgcg tctgcaccgg gctcgggttc gcgctgcgcg ccggcgagcg
    11581 ctggggcctg ctcggcgcca acggcagcgg caagaccacg ctgctgcaca cgctcgccgg
    11641 cctgcgcgcg ccgcaggccg gcgaggtccg cctcgacggt ctgccgctca cggcgctcgc
    11701 gccgcgccgg cgcgcactgc gcctcggcct gctgccgcag gagcatgagg acgccttccc
    11761 ggccaccgtc gccgaaaccg cgctgaccgg gcgctttccg cacctcgggc gcttcggcca
    11821 cgccggagcg gcggaccggg cggcggtcga ggcggcgctg gccgcgaccg ggctggccgc
    11881 gcatgcgcag cgtcaggtcg caacgctgtc cggcggcgag cgccggcggc tcggcttcgc
    11941 gacgctgctg gcgcaggacc cggccgtgct gctgctcgat gagccgacca accacctcga
    12001 cctcggttat caggtgcgcc tgctcgatgc gctggccgcg cgcgtcgccg ccgaggggcg
    12061 cggctggatc atggtcagcc acgacgcgac gctggtcgcg cgcttctgca cgcatgtact
    12121 gctggtctac ggcgatggcc gcaccgccac cggcgggccg cagctgctgt gcccgcagca
    12181 gctgtcggcg ctgtacggcc acccggtgct gcgcctcgac ggaccgctcg gaccggtgtt
    12241 cgcgccgggc tgagggcagc aggcagcgtc tatgctcccg gctcctcacg ctttttcccc
    12301 agccttgcag gagtttcgcc gatgacgctt ccgcccgagg atcgctcccg tcgccagttc
    12361 ctgagcgcgc tcgccgccgc cggcttcgct gcagcggtgc agccggtctg tgccgatacc
    12421 atcctgacgc cgaccgacgg cctcgatgcc ggcatggacc gcgtcgccgc caacgacggt
    12481 ttcctgccgg tctaccgcgc cgtgccgcag ggcaggaaga acctgccgct gatcctggtc
    12541 gtgcaggaga tcttcggcct gcacgagcac atcaaggaca tctgccggcg gctggcgcgg
    12601 ctcggctact gcgcgatcgc gcccgacctg tactaccgcc agggcgacgc gaccaaggtc
    12661 agcgacatcc cgacgctgct gaagaccatc gtcgccttcg tgcccgacga acaggtgctg
    12721 cgcgacctcg ataccgtcgt gcgctgggca cagggcagcg gttattacga tggccaccgg
    12781 ctcggcatca ccggcttctg ctggggcggg cgcatcacct ggctgtactg cgcgcacaac
    12841 ccgcaggtgc gcgccggcgt ggcgtggtac ggcaagctcg aaggcgagga gacggcgatg
    12901 acgccgcagc acccgatcaa tattgccgac tcgctgcagg tgccggtgct cggcctgtac
    12961 ggcgggcagg acgccggcat cccgcaggat tcgctcgggc gcatgcgcag ccgcctcgcc
    13021 tcggcgcaca gccagtccga gatcgtcatc tatccggacg ccccgcacgc gttcaatgcc
    13081 gactaccgcc cgagctaccg caaggctgcg gccgaggacg gctggcggcg catgcgcgag
    13141 tggttctcgg tgcacggcgt caacgtctga agcccggtct gcctgtccgg ccggcaggac
    13201 gcggttccgc cggccgttgc tcccgaaatc cgctcccgcg agggtcgcgt cctgccgctg
    13261 cggctgcgaa tccgcatggc ggtccgcagc cggcaggcgg acgctcacgc gcatttccaa
    13321 cccctgcgcg gagcctgcga tggataccct gaagctgctg ttttcggact ggaccggcat
    13381 cctgtcggcg ctggtcatcc tcttcatgct cggcatgggc gtgtatttcg gctggctgtt
    13441 catcggcagt tcgggcgacg ggccgaagtc ctgatccggg ctgccggccg tcgtgcccgg
    13501 gctgcatcct ccgggaccgg cccggctttc gcgatgcggg ccggtcgacg gacgttcgag
    13561 cgcgtccgcc ggatgcaagg acctgatgca ttcccgcttg cagtgcgccg cttccggttt
    13621 ccggacggtg ccggccggtt tccgcatcct tcaccgccat gccccggtgg tttccggtaa
    13681 ttccgcatca ggatttggca gtgattgcga tccggcggat tccccagtga atctccggcg
    13741 agcttccggg caggaaatcg ccgatgccgc catcggtcgt ccgcatccgg atcgtcatcc
    13801 gatgcgaatg gacaattgcc gcagcgcaag aacggatatc gtttcgcggg agatgtccgg
    13861 ccggagtcgt cccgaatggc gggtccggaa tgccatgagc cgtctgatac ccatcacgac
    13921 ttgaatgccc ggtcatttca ttcccttccc cctcgccggg aagggatggg agagcggggg
    13981 ataaaccacg cattcacgcg ggatcggcct gagcggtaca tcagtgcgca ttgcggaccg
    14041 tgccggattc cgcggtcagc aatggccatg ccgcgaacgg cccgacgcct gtccgccgaa
    14101 acacctgaaa ctgaagggtt tgcagcagtt gcctgccgcc tgcgccggag cgccgcgacg
    14161 cgcgcctccg cgggcaggcg ccgagggcgg acggaccgga tgcgcaccac cggctccgct
    14221 tgcggcctcc ccgcaggtcc accatggcgg cttccggtat ccgcacggca ttgcacggac
    14281 ctcaagccct gcgcagatgc ctgcctatgc agacatccag tatcagccat acggatttaa
    14341 tcgtgagtta gccgcttcgc cggcctcatg ccgatcgaca aagccagagc cggcgcaggt
    14401 ttagcggtgc ggcgagcggc cgcgtgcacg tgtccgccgg ctgtcgcaac cgtgtaacag
    14461 cgtgtaatcg cctgaacttg aacgggcttt ccgggttcat gatcaaaaaa tccataacga
    14521 gaaccctgcc gtgccggtcc cccctgtgtc cgccggtcct gcccgagact tccccccatg
    14581 ccgcaatccc gcaaatccga atggccgctg atcctcgggc tggtgctgcc gttgtcgctg
    14641 gtcgccgtca cgctcagcgg ctgcgatgag ccgcaggcgg ccgtgaagcc ggcggcgccg
    14701 gcgccggtcg aggtcggtgt cgtcgagatc cgccccgagc cgctgacgct gaccaccgaa
    14761 ctgccgggac gcacccgcgc gcatctggtc gccgaggtgc gcccgcaggt cggcggcatc
    14821 atcgagcagc ggctgttccg cgaaggcgcc gaggtcggcg ccggcgatct gctgtaccgc
    14881 atcgatccgg cgagcttcca ggcggcgtac gacagcgcga aggccgcgct cgccaaggcg
    14941 caggccgaca tccactcggt gcagctgaag gccgagcgct acaaggatct ggtgcgcatc
    15001 aaggcggtca gcgatcagga ttacgacgac gcgcgcgccg cgctcgcgca ggcgcaggcc
    15061 gaggtcgagg cccagaaggc cgcgctcgag accgcgcgca tcaacctcga ctacacgcag
    15121 gtgcgcgcgc cgatcgccgg gcgcatcggc cgctcttcgg tgacgccggg cgcgctcgtg
    15181 accgccgacc agaccacggc gcttgcgacc atccagcagc tcgatccggt ctacgtcgat
    15241 gtgcagcgct ccagcaccga atggctggag ctgcagcagg cgctgaaaag cggccggctc
    15301 agccaccccg gtgccgacgg cagcatcacc gtgcgcctga agctcgaaga cggcagcgac
    15361 tacccgcaga ccggcaggct gctgttttcg gaggtcacgg tcgatgccgg caccggtgcg
    15421 atcacgctgc gcgccgaatt cccgaacccg gaccagcgcc tgctgccggg catgtacgtg
    15481 cgcgcggtgc tcgaagaggg catcgcgcag caggcgctgc tggtgccgca gcgcgccgtc
    15541 agccgcgatg cgcgcggcaa ggccagcgcg ctggtggtca atgccgacgg caaggccgag
    15601 cgccgcgatc tggtcaccga gcgcgcggtc ggggaccgct ggctggtgac gaccggcatc
    15661 gccgccggcg agcgcgtgat cgtcgacaac ctgcagaagg tcaaggccgg tgcgctggtg
    15721 aagccgctgc cgtggtcggc gccgaagcgg gaaaccgccg gcctgcgccc gctgaccgat
    15781 ctcgatccga tctgagccgg agcgcgcgac catggcccga ttcttcatcg atcgcccgat
    15841 cttcgcgtgg gtgatctcga tcacgatcat gctcgccggc gcgctggcga tcttcacgct
    15901 gccgattgcc cagtaccccg acatcgcgcc gccgaccgtc gcgatcaacg ccacctatcc
    15961 cggcgcctcg gccaaaacca tcgaggactc ggtgacgcag gtcatcgagc agaacatgaa
    16021 gggcatcgac gggctgcagt acatgagctc gaacagttcg tccgccggca ccgcgcgcgt
    16081 cgagctgacc ttctcggccg acaccaaccc cgacatcgcc caggtgcagg tgcagaacaa
    16141 gctgcagctg gcgctggcgc tgctgccgca ggccgtgcag tcgcagggcg tgaccgtcac
    16201 caagtccgcg aacagcttcc tcgccgtcta cgccttcgtg tccgatcgcg acgacgtgac
    16261 cggcaccgac atcgccgatt tcgtctcgaa ccaggtgctc gacccgatca gccgcatcga
    16321 cggcgtcggc gagacgacgc tgctcggcgc caggtacgcg atgcgcatct ggctcgatcc
    16381 ggcgaagctc gtgaagtacg cgctgacgcc ggccgacgtg aaatcggcga tcgaggcgca
    16441 gaacacgcag gtcgcggtcg gccagctcgg cgacctgccg gccgcgcccg gcaccggtct
    16501 gaacgcgacc atcaacgccc gcagccgcct gcagacgccg gagcagttcc gcgccatcgt
    16561 gctgcgcagc gccaccgacg gcgccaccgt gcgcctgtcc gatgtcgcgc gcgtcgaggt
    16621 cggcgccgaa agttattcgg tgctggcgcg cttcaacggc cgcccggccg ccggcctcgc
    16681 gatccggctc gcctcgggcg ccaacgcact cgcggtcgcc gaggccgccg aggcgaagat
    16741 ccgcgaactg gaacgcttct tcccgtccgg catccgcgcc gtgattgcca acgacacctc
    16801 gccgttcgtg cgcctgtcga tcgaggaagt ggtcaagacg ctgatcgagg cggtcgtgct
    16861 ggtgttcggc gtcatgtacc tgttcctgca gagctggcgc gcgacgctga tcccgaccat
    16921 cgccgtgccg gtcgtgctgc tcggcacctt cggcgtgctc gccgcgctcg gttactcgat
    16981 caacacgctg acgctgttcg cgatggtgct ggcgatcggc ctgctggtcg atgacgccat
    17041 cgtcgtcgtc gagaacgtcg agcgcatgat ggcggagcaa aagctcccgc cgctcgaagc
    17101 cacgcgccgg tcgatggacc agatcagcgg cgcactgatc ggcatcgcgc tggtgctgtc
    17161 ggcggtgttc gtgccgatgg ccttcatcca gagttccagc ggcgtcatct accggcagtt
    17221 ctcggtgacc atcgtctcgg cgatggcgct gtcggtgctg gtggcgctga cgctgtcgcc
    17281 ggcgctgtgc gcgacgctgc tgcggccgct gcccgcaggc caccacgccg gcggccacgg
    17341 ccggctcggg cgcgcgttcg cggccttcaa ccgcggtttc ggccgcacga ccgaccgcta
    17401 tgccgcggcc gccggcggca tcgtgcgccg gccgctgcgc ttcgtgctgc tgtacgtgct
    17461 gctgctcggc gtgctcggcg tgctgttcct gcgcctgccg acgagcttcc tgcccgatga
    17521 ggaccagggc acgctgtatg cgcagatcac gctgccggtc ggcgcgacgc aggagcgtgc
    17581 gctcgacgtg atcgcccagg tcgagaacta cttcctcgac cacgagaagg acgccgtgcg
    17641 ctcggtgttc gcggtttccg gcttcggctt cggcggcaac ggccagaacg tcgcgaccgc
    17701 cttcatcaag ctgcgcgact ggagcctgcg cgaccgtgac gaactgcgcg ccccggcgat
    17761 cgtcgagcgc gcgacgaaga cgctcgggcg catccgcgat gcgcaggtgt tcgtgatgaa
    17821 cccgccgcag atccgcggcc tcggcaactc gaccggcttc gacgtgcaga tgcaggacag
    17881 cggcggcgtc ggtcacgagg cgctggtggc ggcgcgcgag cgcttcctcg aactcgcacg
    17941 ccaggagccg ctgctgacgc gcgtgcgccc gcagggcctc gatgacacgc cgcagctgca
    18001 ggtcgacatc gacaccgcca aggccggcgc gctcgggctg acgatgagcg aggtcaacga
    18061 cgcgctcggc accgcgctcg gcggcgacta cgtcaacgac tatctcgatc gcggtcgcgt
    18121 caagaaggtc tacgtgcagg gcgacgcgcc gttccggatg cggcccgagg acatcagccg
    18181 ctggtacgtg cgcaacggcg acggcgcgat ggtgccgttc tcggacttct cgcatgcgta
    18241 ctgggtctac ggctcgccac ggctggagcg cttcaacggc agctcgtcga tcgagatcgt
    18301 cggcgaggcc gcgccggggc gcagttccgg cgaggcgatg gcggcggtcg agcggatcat
    18361 gggccagatg ccgcagggca tcgcctacga atggaccggc ctgtcgcggc aggagcgcca
    18421 gtccggctcg caggcgccgt atctgtacgc ggtgtcggtg ctcgtggtgt tcctggcgct
    18481 ggcggcgctg tacgagagct ggtcgatccc gttcgcggtg ctgctggtcg tgccgctcgg
    18541 cgtcatcggc gcggtcacgg ccaccgagct gcgcgggctg gagaacgacg tgtacttcca
    18601 ggtcgggctg ctgacgacga tcggcctgtc ggcgaagaac gccatcctga tcgtcgagtt
    18661 cgcgcatgag ctgcagcgca agggcatggg cctgatcgag gcgacgctgt cggccgcgca
    18721 ccagcgcctg cggccgatcg tgatgacctc ggcggcgttc atgctcggcg tgctgccgct
    18781 cgcgatcagc accggcgccg gctccggcag ccggcattcg atcggcaccg gcgtgctcgg
    18841 cggcatgctg tcggcaaccg tgctggcgct tttcttcgtg ccgctgttct tcgtgctggt
    18901 gcgccgctac ctcggcgggc gcgagcgtgc
//
LOCUS       DAOQIE010000028        18329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1404_length_18329_cov_7.415891, whole genome shotgun sequence.
ACCESSION   DAOQIE010000028 DAOQIE010000000
VERSION     DAOQIE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 18329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18329
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_18329_cov_7.415891"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK144_03240"
     CDS             complement(<1..608)
                     /locus_tag="PK144_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64770.1"
                     /translation="MHTDAIVFVVDDDAALRDSLTWLLESVGLRASTHATAQEFLGNW
                     HDPDCAACLLLDVRMPGMGGLQAQSRLPEHGIEIPVIIITGHGDVPMAVAAMKAGAVD
                     FIEKPFNDQLLLDCVQAALARDRQRRAALARRETVHARYTSLTPREREVMREVVRGHA
                     NKDIAETLNVSRKTVEVHRAKVMDKMQAESLSQLIQMAMSLG"
     gene            complement(676..2523)
                     /locus_tag="PK144_03245"
     CDS             complement(676..2523)
                     /locus_tag="PK144_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64771.1"
                     /translation="MTALRPPGPAAPFARLAEHLPDPVFACNSDGSVLAYNPAAAGLA
                     GTVGLDAPEQLLPARHVEYVHECLYHDEIAELADTRVGPFTLCWTYVPSGGGTAVHVL
                     GRDVTDSRRTEAALRISEARHRLLAEQSTDIISRHSPGTWTFLYASPAAQTLLGYAPE
                     ELVGRSSLDFFHPEDRRAYQERGPSVAYERGIYVATYRYRHKDGHYVYLESTSRTLRD
                     PVSGELLEIISVSREVSRRVLAERATKRLARIVEATTDLVAFFEPNGRVTYLNEAARR
                     VLGIPYPENKCLKLAELMPAAAYAQIEREALPTARLGGVWSGETAFVDAMGREIPVSQ
                     VVLAHAGEHDEIDYFSTIARDISERRQAEEREQRHQAEMTHVARLITMGEMASGLAHE
                     LNQPLAAIVNYARGAIRRIHGEPPAELPVLAGVLERIAEQASRAGEIIKRLRSFARKS
                     EFRRAPVDLNKVVGDMIRFCRPEARRHALELRAELTGTLPAVLADRVQIEQVLLNLLR
                     NAMEATVQAQGSGHGPSTHDASLRTVTLSSACLPGRVVAVHVADHGHGLPPGGPEPLF
                     EQFYTTKPKGLGLGLSISRSIMEAHGGALWAENRPGGGAVFSLSLPAAP"
     gene            2831..3148
                     /locus_tag="PK144_03250"
     CDS             2831..3148
                     /locus_tag="PK144_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNF64772.1"
                     /translation="MSLTPSPSERIMACPRFARLVAARSRFAWTLTAVVLGIYYGYMM
                     VVSFAPQLLAAPLSDGGTLPVGVPVGAAIIVLSWVLTGVYIRRANSEFDALTEQVLEE
                     HAP"
     gene            3202..4824
                     /locus_tag="PK144_03255"
     CDS             3202..4824
                     /locus_tag="PK144_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNF64773.1"
                     /translation="MLPLALLAAPAGPVEVQKQPVNVTAIVMFMIFVAMTMGITYWAA
                     SRTRSAKDFYTAGGGITGFQNGLAIAGDYMSAATLLGLSGLVFARGHDGFIYAIGFFV
                     GWPVIMFLMAERLRNLGKYTFADIVSYRLEQTPIRTLAASGTLAVVAFYLIVQMVGAG
                     KLIQLLFGLEYWMAVVLVGVIMVVYVTFGGMLATTWVQIIKACLLLGAGTFMFVLAMS
                     QFGFSFETLAQKAVAAHKSGIKLMAPGALLADPVSSVSLSLGLVFGLAGLPHIMMRFF
                     TVTNAREARKSVFYANGFIGYFFLIVCAMGLTAIVIVGTNPEYLDAAGKLKGGENMAA
                     MHLAKAVGGDLFLGFISAVAFATILAVVAGLALAGASAVSHDLYASVFKRGQADEAAE
                     MRVSKIATVVIGIVAIVLGILFENQNVAFMTALTFGVAASANFPVLILSMYWKGLTTR
                     GALIGGFTGLLSAVGFVVMGPTVWVKVLGNAAPLFPYDNPALFSMPLAFATIIIISKL
                     DGSARAARERAAYAAQFVRAQTGLGAEAAAEH"
     gene            5164..6810
                     /locus_tag="PK144_03260"
     CDS             5164..6810
                     /locus_tag="PK144_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNF64774.1"
                     /translation="MHHNPYELGLDQNPANFTALSPLSFIERTASIYPERTATVHGDV
                     RRTWGETFARCRRLASALKKRGIGAGDTVAVMLPNIPEMLEVHFGVPASGAVLNTLNV
                     RLDAEAIAFMLQHGEAKVLITDREFTDTIRKALHMLQTRRPLVIDVDDLQYSGGALLG
                     TLTYEDLLAEGDEDAPWVLPTDEWSAITLNYTSGTTGNPKGVVYHHRGAYLNAMCNIV
                     AWDMGKHPVYLWTLPMFHCNGWCFPWTLAAAAGVSVSLRAVRPEPIFKAIKEEKVNHF
                     CGAPIVLNMLNNAPAALKDGITHQVKVMTAGAAPPAAVIEGMERMGFQVTHVYGLTET
                     YGPCVSCAWQDRWNDESIEEKARLKARQGVRYPMQEGLMVADPTNLEPVPQDGKTMGE
                     VFMRGNLVMKGYLKNPSATEESFQGGWFHSGDLAVWHEDGYIEIKDRSKDIVISGGEN
                     ISTIEVEDVLYRHPAVMEAAVVARPDSKWGETPCAYVTLKPEKEGEVQESEIIAFCKT
                     QMAGYKVPKTVIFGPLPKTSTGKIQKFVLRNQAKELGSLS"
     gene            complement(7044..7478)
                     /locus_tag="PK144_03265"
     CDS             complement(7044..7478)
                     /locus_tag="PK144_03265"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF64775.1"
                     /translation="MKIRNSGSAVWSGGLKDGKGAISTQSGALDAHPYGFAMRFEGVP
                     GTNPEELIGAAHAGCFTMALSKILGEAGFTAERMETTAVVTLEQVGEGFSITGVALTL
                     RATVPELEAAQFQELAAKAKAGCPVSKLLKAEITLDAQLLTA"
     gene            complement(7593..7874)
                     /locus_tag="PK144_03270"
     CDS             complement(7593..7874)
                     /locus_tag="PK144_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64776.1"
                     /translation="MIRNDAWLADLRARLDDWSGRIEQLDQQARHADPARRARLEEQL
                     VILRRLREDTENRLQTLLRATGTEPPPVLRARSSGAWQRRHQAFERLHG"
     gene            complement(8094..9392)
                     /gene="guaD"
                     /locus_tag="PK144_03275"
     CDS             complement(8094..9392)
                     /gene="guaD"
                     /locus_tag="PK144_03275"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNF64777.1"
                     /translation="MTELRAFRAELLDFTGDPGENPDSAHVRHRPDGLLLVADGRIVE
                     CGPAARLLPQLSPEVPVEYFPDRLLMPGFVDAHIHYPQTDIIASYGSDLLDWLQTYTY
                     PAERAFADPAHAHELAGVFIEELLRHGTTTALVMASVHPQSVDALFTAAAGQRLRLVA
                     GKLLMDRHVPEDLCDPADAGYADSLALIRRWHGHGRFHYAITPRFAPTSTEGQLDVCG
                     RLARLDDSLFIHSHVAESQRELEWVAGLFPWARSYLDVYDRFGLLRRRAVYAHCLHLD
                     AADRTRMCESGASVAFCPGSNLFLGSGLLDLPACAAGHLALGTDVGGGTSFSLLHTLA
                     EGYKVARLAGHTLAPLRGFYLATFGGARALDLDAHIGSFEPGREADFVVLDPGATQLL
                     TRRTARAVSLAERLFALMMLGDDRAIARVYVLGECVHAIG"
     gene            complement(9675..10787)
                     /locus_tag="PK144_03280"
     CDS             complement(9675..10787)
                     /locus_tag="PK144_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF64778.1"
                     /translation="MFSPRRRQLLQFAAATPLAAAFASVPRPARAAFPKPELPLKVGY
                     VYVSPIGDAGWTYQHDLGRKAVEASFGDKVMIKVIESVSEGADAERVIREFASSGYGL
                     VFATSFGYMNPLLRVAKQFPKGIFEHATGYRQAPNVGIYAARYYEARYLTGILAGRMS
                     KSGVAGYVAAFPIPEVVMGINAFMLGMKSVNPQAQVKVIWVNAWFDPGKEREAAATLV
                     AQGADVLTQHTDSTAVVQTAEDKGAYAIGYHSDMSKYGPKAHLSAATHHWEGYYRRVV
                     QQVLDGSWAPDNVWGGIKDGMVGLAPLHPAVPAEVGALVATKKQDIIDGRLLPFQGPL
                     FDQAGRQVIGAGAKLTDDELLKMNYYVEGVVGQMPK"
     gene            complement(10967..11863)
                     /locus_tag="PK144_03285"
     CDS             complement(10967..11863)
                     /locus_tag="PK144_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64779.1"
                     /translation="MSLSGWLPILAGVLAAATPLLIAALGELIAEKSGVLNLGVEGMM
                     LVGALTAFAVASGGAGYAAGALAGALAGVGLAALFAWLTLNCLASQVACGLAITMLGS
                     GLSAYAGRDYVSRTLDGMALIPLGGGFGFDPLIVTGLLLTVLVWYFLQRSRAGLVLRA
                     VGESPATAHALGLPVRRIRWAAVLFGGACAGLAGAYLAIDYTPMWVEGLSAGRGWIAL
                     ALVVFAGWRPGRTLLGAWLYGGVGILQFHAQALGIAVPAQFLGMLPYLATIVVLTLIA
                     RDPLTQRRHTPASLGQPWHPHA"
     gene            complement(11860..12948)
                     /locus_tag="PK144_03290"
     CDS             complement(11860..12948)
                     /locus_tag="PK144_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64780.1"
                     /translation="MFTLEARTLPSVRAMWLAPLAALLLTLLGGSVLFAAFGHAPLQA
                     LSVYFLQPLASVYGIGEWLLKATPLALCGLGLALGFRAKVWNIGADGQFTLGAICAGG
                     LAIACDGFIDAESLWIAPAMALAGIAGGMMWAALPAWLRTRYGSSEILSSLMLAYVAP
                     LLLGWLVNGPWRDPAGFNFPQSIQFADAALLAPLHEGARVTLAFPVALVLAGLLCFVQ
                     RYTYAGFRLQVSGEAPLAARHAGYSQRAAVWIAMLTGGACAGFAGYAEVAGPLGQLQP
                     SVSPGYGFTAIIVAFVGRLDPRGVVLAALLLSLLALGGEAAQMTLDLPAAISGVFQGG
                     LLCALLACDTFVHYRLRRVYRKPAARTS"
     gene            complement(12935..14488)
                     /locus_tag="PK144_03295"
     CDS             complement(12935..14488)
                     /locus_tag="PK144_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64781.1"
                     /translation="MSATIPRLELRAIRKHYSGTIANDDIDLVVHPGEIHAVLGENGA
                     GKSTLMKIIYGVVAPDAGELFWDGQPVRIHSPAQARRLGIGMVFQHFSLFESLSVAEN
                     IALALPDAPRPAALAGRIRRVSADYGLALEPMQPVHELSVGERQRVELVRCLLQSPRL
                     LILDEPTSVLTPQAVEALFVTLRRLAREGVSLLYISHKLEEIRRLCDQATVLRNGRVR
                     AHCRPAEESAASLARLMIGGELPATRLTPNALPRTPRLVLDRLSRTADGPFGTPLREL
                     ELCVHSGEIVGIAGASGNGQKELLEALFGEVALADAAMLQLAGEDIGRLGPAARRARG
                     LGMVPEERLGRAAVPARSLADNTLLTAAGHGLVRAGLIRRPAVLRFARALIEGFQVRC
                     KGAQAPANSLSGGNLQKFIVGRELAQHPQALVIAQPTWGVDVASAALIHEQLLRLRND
                     GVALLVISEDLDELFGLCDRIAVLAGGRLSPVRETGATDAAELGQWMGGGFGPVTAAA
                     APGMRDVHA"
     gene            complement(14749..14949)
                     /locus_tag="PK144_03300"
     CDS             complement(14749..14949)
                     /locus_tag="PK144_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64782.1"
                     /translation="MTDDLRILADDSRVMMDEAPVMTGELGFMSDELQLMSDESRFMS
                     DESRVMIHEFRVRTDESRFMPD"
     gene            15395..15760
                     /locus_tag="PK144_03305"
     CDS             15395..15760
                     /locus_tag="PK144_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64783.1"
                     /translation="MANIRSKDFLSDMERKINSIRQFASDRVFGGVTLADLESARTAS
                     LNARTDIQHAENAMIALRNHRQESDRHYEELMRRAVAGLIADPDFGPDSDAYDAMGYT
                     RYKERKRRTTKPRREPEDS"
     gene            15963..16100
                     /locus_tag="PK144_03310"
     CDS             15963..16100
                     /locus_tag="PK144_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64784.1"
                     /translation="MNDPVPVTAEGDDEDQIDGCDVDCDGEATEYTPDEELPASDGGV
                     A"
     gene            16102..16902
                     /locus_tag="PK144_03315"
     CDS             16102..16902
                     /locus_tag="PK144_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNF64785.1"
                     /translation="MAWSLIWLPEILKRAGLKVALVDGWENRGRGDMGRVEGVICHHT
                     VGSPNLNMPSLKVLINGRADLPGPLAQLGLGRDGTYYVIAAGRCNHAGKGSWNGQTHG
                     NSNFIGIEAENTGKADDLPWPEVQMDAYRRGVAAILKHVGKGADGCAGHKEYALPKGR
                     KPDPSFDMPAFRNEVAAILNGSAPAPVLIPAFEPATASTNSGAARPTLRRGASGELVK
                     HLQQKVGASVDGDFGPKTEAAVRVFQRQKGMVPDGIVGPKTWAAMDAV"
     gene            complement(17003..17524)
                     /locus_tag="PK144_03320"
     CDS             complement(17003..17524)
                     /locus_tag="PK144_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HNF64786.1"
                     /translation="MSFSFQCSICGQVHEGMPSFGAAAPLNYYQIPVEEREARCALGS
                     DDCVIDGNFFFVRGCIEIPVHGQDEAFNWGVWVSLSQASYLNWLKYFNEPRRAHIGPF
                     FGWLNAWLHPYPDTVNLKTQIHLRDDGIRPYIELEPTDHPLAIEQRTGISTERVAEIY
                     ALIVHEDDASSAG"
     gene            complement(17605..18045)
                     /locus_tag="PK144_03325"
     CDS             complement(17605..18045)
                     /locus_tag="PK144_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF64787.1"
                     /translation="MQWSKTDYIVSTNPEDQDLDVIHGFLSQSYWARNIPRETVARSL
                     QGALCFALLHKGRQIGFARVISDSATIAYLGDVFVLPEYRGRGLAGWLLECVTAHPQL
                     QGLRRWILATADAHGLYEKFGFTALKRPEIFMELHDPEVYSADR"
BASE COUNT         3084 a         6114 c         6226 g         2905 t
ORIGIN      
        1 tcgccgaggc tcatcgccat ctggatcagc tgcgacagcg actcggcctg catcttgtcc
       61 atgaccttgg cgcggtgcac ctcgacggtc ttgcgcgaga cgttcagcgt ctcggcgatg
      121 tccttgttgg cgtggccgcg cacgacctcg cgcatgactt cgcgctcgcg cggcgtcagc
      181 gaggtgtagc gcgcatgcac ggtctcgcgc cgggccagcg ccgcgcggcg ctggcggtcg
      241 cgggcgagtg cggcctgcac gcagtcgagc agcaactggt cgttgaacgg cttctcgatg
      301 aagtcgacgg caccggcctt catcgccgcg accgccatcg gcacgtcgcc gtggccggtg
      361 atgatgatca ccggaatctc gatgccgtgc tccggcagcc ggctttgcgc ctgcaggccg
      421 cccatgccgg gcatgcgcac gtcgagcagc aggcaggccg cacagtccgg gtcgtgccag
      481 ttgccgagga attcctgcgc ggtggcatgg gtgctggcgc gcaggccgac cgattccagc
      541 agccaggtca gggaatcgcg cagcgcggcg tcgtcgtcga cgacaaagac gatggcatcg
      601 gtatgcatgg cgggcggact cgcgggctcg gggggagggc ggcggacgtg gggagttgat
      661 gatacggcag cgcgttcatg gtgctgccgg cagcgacagg ctgaacaccg cgccgccgcc
      721 ggggcggttt tcggcccaca gcgcaccgcc gtgggcctcc atgatcgagc ggctgatcga
      781 aaggccgagg ccgaggccct tgggcttggt cgtgtagaac tgttcgaaca gcggctcggg
      841 gccgccgggc ggcaggccgt ggccgtgatc ggcgacgtgc acggcgacga ccctccccgg
      901 caggcatgcg ctcgacagcg tcaccgtgcg cagcgacgca tcgtgcgtgc tcgggccgtg
      961 gccgctgccc tgggcctgca cggtggcttc catggcgttg cgcagcaggt tcagcagcac
     1021 ctgctcgatc tgtacgcgat cggcgagcac cgccggcagc gttccggtga gttcggcgcg
     1081 cagttcgagc gcgtggcggc gcgcctcggg ccggcagaag cggatcatgt cgccgacgac
     1141 cttgttcaga tcgaccgggg cgcgccggaa ctcgctcttg cgcgcgaagc tcctgagccg
     1201 cttgatgatc tcgccggccc ggctcgcctg ctcggcgatg cgctccagca cgccggcgag
     1261 caccggcagt tcggccggcg gctcgccgtg gatgcggcgg atcgcgccgc gcgcgtagtt
     1321 gacgatcgcc gccagcggct ggttcagctc gtgcgcgagg cccgaggcca tctcgcccat
     1381 cgtgatcagg cgcgcgacgt gcgtcatctc ggcctgatgg cgctgctcgc gctcctcggc
     1441 ctgacgccgt tcgctgatgt cgcgggcgat ggtcgagaag tagtcgatct cgtcgtgctc
     1501 gccggcgtgc gcgagcacga cctgcgagac cgggatctcg cgccccatcg catcgacgaa
     1561 ggctgtctcg ccgctccaga cgccgccgag gcgtgccgtc ggcagcgcct cgcgctcgat
     1621 ctgcgcgtag gcggcggccg gcatcagctc ggcgagcttc aggcacttgt tctcgggata
     1681 cggaatgccg agcacgcgcc gggcggcctc gttgaggtag gtgacgcggc cgttcggctc
     1741 gaagaaggcg acgagatcgg tggtcgcctc gacgatgcgc gccagacgct tggtggcgcg
     1801 ctcggcgagc acgcgccggc tgacctcgcg cgataccgag atgatctcca gcagctcgcc
     1861 gctgaccgga tcgcgcagcg tgcggctggt gctctccagg tacacgtagt gaccgtcctt
     1921 gtgccggtag cggtaggtcg cgacgtagat gccgcgctcg taggcgacgc tcgggccgcg
     1981 ctcctgatag gcgcgccggt cctcggggtg gaagaaatcc agcgaggacc tgccgacgag
     2041 ttcctcgggc gcgtagccga gcagcgtctg cgcggccggc gaggcgtaca ggaaggtcca
     2101 ggtgcccggc gagtggcgcg agatgatgtc ggtcgactgc tcggcgagca ggcggtggcg
     2161 tgcctcgctg atgcgcagcg ccgcctcggt gcggcggctg tcggtcacgt cgcgcccgag
     2221 cacgtgcacg gccgtgccgc cgccggaggg cacgtaggtc cagcacagcg tgaacgggcc
     2281 gacgcgggtg tcggcgagtt cggcgatctc gtcgtggtac aggcattcgt gcacgtactc
     2341 gacgtggcgt gccggcagca gctgctccgg tgcatcgagc ccgaccgtcc cggcgaggcc
     2401 ggcagcggcc gggttgtacg cgagcacgct gccgtcactg ttgcaggcga acaccgggtc
     2461 cggcaggtgc tcggcgagcc gtgcaaacgg cgccgccggg ccgggggggc gaagcgcagt
     2521 catcaggtct tgctccacgc tcgaagcgat ataggtgaaa accctagtat gcctagctag
     2581 tgtccgaagg ttgttttcag tattcttccg aaaataccta gacatcttcg catgcaggag
     2641 atgcacgcat gacgggcgga cggttcacga gaccgttcgt gaccggcatg acgaagcgcg
     2701 gaccgcgcgt cgcccaccgg cgcgcacgga gcgcgcacaa cgacagaaaa cacaccacaa
     2761 gcacacaacc ccagaacgac tggagcagcg gtccggctgc gggccgcaaa gacctggaga
     2821 gcatcgaaac atgtccctga ccccgtctcc gtccgaacgg atcatggcct gtccgcgctt
     2881 tgcgcggctg gtcgcggccc gttcgcgctt tgcctggacg ctgaccgccg tcgtgctcgg
     2941 catctattac ggctacatga tggtcgtgtc cttcgcgccg cagctgctgg ccgcaccgct
     3001 gagcgacggc ggcacgctgc cggtcggcgt gccggtcggt gccgcgatca tcgtgctgtc
     3061 ctgggtgctg accggcgtct acatccgccg cgccaacagc gagttcgacg cgctgaccga
     3121 gcaggtgctc gaggagcacg cgccatgatc cacagtggca accgcttcat tccgcgcctg
     3181 ttcctgctcg ccctcggcct gatgctgccg ctggccctgc tcgccgcccc ggccggtccg
     3241 gtcgaagtgc agaagcagcc ggtcaacgtc accgcgatcg tgatgttcat gatcttcgtc
     3301 gcgatgacga tgggcatcac ctactgggcc gcctcgcgca cgcgctcggc caaggacttc
     3361 tacaccgccg gcggcggcat caccggcttc cagaacggtc tggcgatcgc cggcgactac
     3421 atgtcggcgg caacgctgct cgggttgtcg ggcctggtgt tcgcacgcgg ccatgacggc
     3481 ttcatctacg cgatcggctt cttcgtcggc tggccggtga tcatgttcct gatggccgag
     3541 cgcctgcgga acctcggcaa gtacaccttt gccgacatcg tctcgtaccg gctcgaacag
     3601 acgccgatcc gcacgctggc cgcctccggc acgctggcgg tggtggcctt ctacctgatc
     3661 gtgcagatgg tcggtgccgg caagctgatc cagctgctgt tcgggctcga atactggatg
     3721 gccgtcgtgc tggtcggcgt gatcatggtc gtctacgtga ccttcggcgg catgctcgcg
     3781 accacctggg tgcagatcat caaggcctgc ctgctgctcg gggccggcac cttcatgttc
     3841 gtgctggcga tgtcgcagtt cggcttcagc ttcgagacgc tggcacagaa ggccgtcgcc
     3901 gcgcacaagt ccggcatcaa gctgatggcc cccggtgcgc tgctcgccga tccggtgtcc
     3961 tcggtgtcgc tgtcgctcgg cctggtgttc ggtctcgccg gcctgccgca catcatgatg
     4021 cgcttcttca cggtcacgaa cgcccgcgag gcgcgcaagt ccgtgttcta cgccaacggc
     4081 ttcatcggct acttcttcct gatcgtctgc gcgatgggcc tgaccgccat cgtcatcgtc
     4141 ggcaccaatc ccgagtacct ggacgcggcc ggcaagctca agggcggcga gaacatggcg
     4201 gcgatgcatc tggccaaggc cgtcggtggc gacctgttcc tcggcttcat ctcggccgtg
     4261 gccttcgcga ccatcctcgc cgtggtcgcc ggcctcgcgc tcgccggtgc ctcggccgtg
     4321 tcgcacgacc tgtacgcgag cgtgttcaag cgcggccagg ccgacgaggc cgcggagatg
     4381 cgcgtctcga agatcgccac cgtcgtcatc ggcatcgttg cgatcgtgct cggcatcctg
     4441 ttcgagaacc agaacgtcgc gttcatgacc gcgctgacct tcggcgtcgc ggcctcggcc
     4501 aatttcccgg tgctgatcct gtcgatgtac tggaaggggc tgaccacgcg cggcgcgctg
     4561 atcggcggct tcaccggcct gctcagcgcc gtcggcttcg tggtcatggg gccgaccgtg
     4621 tgggtcaagg tgctcggcaa cgccgcgccg ctgttcccgt acgacaaccc ggcgctgttc
     4681 tcgatgccgc tggccttcgc caccatcatc atcatctcga agctcgacgg cagcgcccgc
     4741 gccgcacgcg agcgcgccgc ctatgccgcg cagttcgtgc gtgcgcagac cggtctcggt
     4801 gccgaggcgg ccgccgagca ctgagccaga gcggctgcta cggtaggcga ccggtcaccg
     4861 tgcacaaggc gggtggccgg tcgatgacca cggtcggtca cgcagcggca ccggatgcag
     4921 gccagccgcc ggggggaggc tcgcatgatt cgtccggttg ccgctcgcct gccagggcag
     4981 cggcaggggc tgcgccggcc tgcccgcgca ggccggcccg cagaagaaag caagcacgcg
     5041 caggctttga tgcctgcccc gacccgcgcc aaccccggtg gcagggccgc tgatcgcggc
     5101 agcgccaccg gattcaccgg gcaagcgcaa cgacccgtcc cgggtcagca ggagtgagga
     5161 acgatgcacc acaacccgta tgaactgggg ctcgaccaga accccgcgaa cttcacggcg
     5221 ctgtcgccgc tgagcttcat cgagcgcacg gcgtcgatct atccggagcg cacggcaacc
     5281 gtgcacggcg acgtgcgccg cacctggggc gagaccttcg cgcgctgccg gcgcctcgcc
     5341 tcggcgctga agaagcgcgg catcggcgcc ggcgacacgg tcgcggtgat gctgccgaac
     5401 attcccgaaa tgctcgaagt gcacttcggc gtgccggcct cgggggcggt gctgaacacg
     5461 ctgaacgtgc gtctcgacgc cgaggccatc gccttcatgc tgcagcatgg cgaggcgaag
     5521 gtgctgatca ccgaccgcga gttcaccgac acgatccgca aggccctgca catgctgcag
     5581 acgcgccggc cgctggtcat cgatgtcgat gacctgcagt acagcggcgg cgcgctgctc
     5641 ggcacgctga cctacgagga tctgctcgcc gaaggcgacg aggatgcgcc gtgggtgctg
     5701 ccgaccgacg aatggtcggc gatcacgctc aactacacct ccggcaccac cggcaatccg
     5761 aagggcgtgg tctatcacca ccgcggtgcc tacctgaacg cgatgtgcaa catcgtcgcc
     5821 tgggacatgg gcaagcaccc cgtgtacctg tggacgctgc cgatgttcca ctgcaacggc
     5881 tggtgcttcc cgtggacgct ggccgcggcc gccggcgtgt cggtgagcct gcgcgccgtg
     5941 cgccccgagc cgatcttcaa ggcgatcaag gaagagaagg tcaatcactt ctgcggcgcg
     6001 ccgatcgtgc tgaacatgct gaacaacgcc ccggcggcgc tgaaggacgg catcacgcat
     6061 caggtcaagg tcatgaccgc cggcgccgcg ccgccggccg ccgtcatcga gggcatggag
     6121 cgcatgggct tccaggtcac gcacgtctac ggcctgaccg agacctacgg cccctgcgtg
     6181 tcctgcgcct ggcaggatcg ctggaacgac gagtcgatcg aggagaaggc gcgcctcaag
     6241 gcccgtcagg gcgtgcgcta tccgatgcag gaaggcctga tggtcgccga tccgaccaac
     6301 ctcgaaccgg tgccgcagga cggcaagacc atgggcgagg tgttcatgcg cggcaacctg
     6361 gtcatgaagg gctacctgaa gaacccgagc gccaccgagg aatccttcca gggcgggtgg
     6421 ttccattccg gtgacctcgc cgtctggcac gaggacggct acatcgagat caaggaccgc
     6481 tcgaaggaca tcgtcatctc cggcggcgag aacatctcga cgatcgaggt cgaggatgtg
     6541 ctctaccgcc atccggcggt catggaagcc gcggtcgtcg cccgcccgga cagcaagtgg
     6601 ggcgagaccc cgtgcgccta cgtgacgctg aagccggaga aggaaggcga ggtgcaggag
     6661 tccgagatca tcgccttctg caagacgcag atggccggct acaaggtgcc gaagaccgtc
     6721 atcttcggcc cgctgccgaa gacctcgacc ggcaagatcc agaagttcgt gctgcgtaac
     6781 caggcgaagg aactcggctc gctgagctga gcctgcagcg gccggcacgc accggctgca
     6841 agaccgccat gctgccggtg cagtggatcg cctgcacaag gacgccccgc attcgcgggg
     6901 cgtcgtcatt catggcgcct gtgccgctgc ggatctgcag aaggccggtc ccggcaggcg
     6961 cggggtttcc gggccttcgc cgttcggtga caggggatcg gggggagggg agattcccgg
     7021 cccgcccgcc tgccggcctg cgctcaggcc gtcagcagct gcgcatcgag cgtgatctcg
     7081 gccttcagca gcttcgagac cgggcagccg gccttggcct tggcggcgag ttcctggaac
     7141 tgcgccgcct ccagctccgg caccgttgcg cgcagcgtca gtgcgacgcc ggtgatcgaa
     7201 aagccctcgc cgacctgttc gagcgtgacc acggccgtcg tttccatgcg ctcggccgtg
     7261 aagccggcct cgccgaggat cttcgacagc gccatcgtga agcagccggc gtgcgccgcg
     7321 ccgatcagct cctcgggatt ggtgccgggc acgccctcga agcgcatcgc gaagccgtaa
     7381 gggtgcgcat cgagcgcacc gctctgcgtc gagatcgcgc ccttgccgtc cttcaggccg
     7441 ccgctccaga cggccgaacc gctgttccgg atcttcatcg gatcatgtct cctcgttcga
     7501 tggcatccgg cgctgccgga tgaggtgctg ccggccgggc ggccggcggc ggaaagcagg
     7561 cggatcagca ggatgccggc ctgcaggcgg tgtcagccat gcaggcgctc gaaagcctga
     7621 tgccggcgct gccaggcacc gctgctgcgc gcgcgcagca cgggcggagg ctcggtgccg
     7681 gtggcgcgca gcagcgtctg caggcggttt tcggtgtcct cgcgcagccg gcgcaggatc
     7741 acgagctgtt cctcgagccg cgcgcggcgc gcgggatcgg cgtgacgggc ctgctggtcg
     7801 agctgctcga tcctgccgct ccagtcatcg aggcgcgcgc gcaggtcggc gagccaggca
     7861 tcgttgcgga tcatcggttc gttctccggt tttctgtcga acacgatgcg ttccgacgcc
     7921 gggtgtgcgg cagtggttcg ctgcgcgggc gcacaaagca atgcgaaccg ctgccggtga
     7981 agcgggtgat tcaaggcatg aacatgattt tcaaggggaa tgttctgtaa tgcttccttg
     8041 acgcgatggc tgcacgcact gcagccatac gcggcgatgc cctgcggcgc gcctcagccg
     8101 atcgcgtgca cgcactcgcc gagcacgtag acccgggcga tggcgcggtc gtcgccgagc
     8161 atcatcagcg cgaacagccg ttcggcgagc gacacggcac gggcggtgcg ccgcgtcagc
     8221 agctgcgtcg cgcccggatc gagcacgacg aagtcggcct cgcgtccggg ttcgaagctg
     8281 ccgatgtgcg catcgagatc gagtgcgcgg gcgccgccga aggtcgcgag atagaacccg
     8341 cgcagcggcg ccagcgtgtg tccggccagc cgcgccacct tgtagccctc ggccagcgtg
     8401 tgcagcagcg agaagctcgt gccgccgccg acatcggtgc cgagggccag atgcccggcg
     8461 gcacaggccg gcagatcaag caggccgctg ccgaggaaca ggttcgagcc cgggcagaac
     8521 gcgacgctgg cgccggattc gcacatgcgc gtgcgatcgg cggcatcgag gtgcaggcag
     8581 tgcgcgtaga cggcgcgccg gcgcagcagg ccgaagcggt catagacatc gagataactg
     8641 cgcgcccacg gaaacaggcc ggcgacccat tcgagctcgc gctggctctc ggcgacatgc
     8701 gaatggatga acaggctgtc gtcgaggcgc gcgagccgcc cgcagacatc gagctggccc
     8761 tcggtcgagg tcggcgcgaa gcgcggcgtg atcgcgtagt ggaagcgccc gtggccgtgc
     8821 cagcgccgga tcagcgccag cgagtcggcg tacccggcgt cggccgggtc gcagagatcc
     8881 tccggcacgt ggcgatccat cagcagcttg ccggcgacca gccgcagccg ctgtccggcc
     8941 gcggccgtga acagcgcatc gaccgactgc ggatgcacgc tggccatcac cagcgccgtg
     9001 gtcgtgccgt ggcgcagcag ttcctcgatg aacaccccgg cgagctcgtg cgcgtgcgcc
     9061 ggatcggcga aggcgcgttc ggccggatag gtgtaggtct gcagccagtc gagcaggtcg
     9121 ctgccgtagc tggcgatgat gtcggtctgc gggtaatgga tgtgcgcatc gacgaagccc
     9181 ggcatcagca gccggtccgg gaagtactcg accggcactt cgggcgacag ctgcggcagc
     9241 agccgcgcgg ccggcccgca ctcgacgatg cggccatcgg caaccagcag caggccgtcg
     9301 ggccggtggc gcacatgcgc cgaatcgggg ttctcacccg ggtcgccggt gaagtccagc
     9361 agttcggcac gaaacgcacg cagctcggtc atggcggcac cgcagggctt cagcgccgcc
     9421 ggctgcggca cgccggagaa gcttaggtgc ttgtccgcca tgccggcatg tccgcgatac
     9481 agtcaggccg gcatgagtca tttcaaaatg acaaatcccg attgaaagcc ttgtcgctgg
     9541 cctgcgatag tgcgggggcg gggcgttccc acgggggctc tcgccagggc tgccgcccgc
     9601 gctggtcggg gggccggcgc gggcggcgct ctgtctgatc cggatgcaga cagccgccgc
     9661 cgctgccggt cacttcactt cggcatctgc ccgaccacgc cctcgacgta gtaattcatc
     9721 ttcagaagct cgtcatcggt gagcttcgcg ccggcaccga tgacctgccg gccggcctga
     9781 tcgaacagcg gcccctggaa gggcagcagc ctgccatcga tgatgtcctg cttcttcgtc
     9841 gccaccagcg ccccgacctc ggccggcacc gccggatgca gcggtgccag tccgaccatg
     9901 ccgtccttga tgccacccca gacattgtcc ggcgcccagc tgccgtcgag cacctgctgc
     9961 acgacccggc ggtaatagcc ctcccagtga tgggtcgccg cgctcagatg cgccttcggg
    10021 ccatatttgc tcatgtccga gtgatagccg atcgcgtacg cgcccttgtc ctcggcggtc
    10081 tgcacgacgg cggtcgaatc ggtgtgctgc gtcagcacgt cggcgccctg cgcgaccagc
    10141 gtcgcggccg cctcgcgctc cttgcccgga tcgaaccagg cgttcaccca gatcaccttg
    10201 acctgcgcct gcgggttgac gctcttcatg ccgagcatga aggcgttgat gcccatcacc
    10261 acctccggga tcgggaaggc ggcgacgtag ccggcgaccc cggacttcga catgcggccg
    10321 gcgaggatgc cggtcagata gcgcgcctcg tagtagcgcg ccgcgtagat gccgacgttc
    10381 ggtgcctgcc ggtagccggt cgcgtgctcg aagatgccct tcggaaactg cttcgcgacg
    10441 cgcagcagcg ggttcatgta gccgaacgag gtcgcgaaca cgagcccgta gccgctgctc
    10501 gcgaactcgc ggatcacgcg ctcggcatcg gcaccctcgc tgacgctctc gatgaccttg
    10561 atcatcacct tgtcgccgaa gctcgcctcg accgccttgc ggccgagatc gtgctgatag
    10621 gtccagccgg cgtcgccgat cggactgacg tagacgtagc cgaccttcag cggcagttcg
    10681 ggcttcggga aggccgcccg ggccgggcgc ggcacactgg cgaaggcggc ggcgagcggg
    10741 gtggcggcgg caaactgcag caactggcga cggcggggac tgaacatgct tcggaaccgg
    10801 ttccttggga catggggcct tgcggacgat ggggccggag gacgcgggcg gggttgcgct
    10861 ccggcaccgg acgggcgctt ccggggtttg cggctttgca gcatggtgat tttatcgccg
    10921 gatgcgggcg ctgcctgcgc aagcatgctg acgcggcggg ccggcttcag gcatgcggat
    10981 gccagggctg tccgagactc gccggcgtgt ggcgacgctg cgtgagcggg tcgcgcgcga
    11041 tcagcgtcag cacgacgatg gtcgccagat acggcagcat gccgaggaac tgcgccggca
    11101 cggcgatgcc gagcgcctgc gcatggaact gcaggatgcc gacgccgccg tacagccacg
    11161 ccccgagcag cgtgcgcccc ggacgccagc cggcgaacac caccagcgcg agcgcgatcc
    11221 agccacggcc ggcgctgagc ccctcgaccc acatcggcgt gtagtcgatc gcgagatacg
    11281 ccccggccag ccccgcgcag gccccgccga acagcacggc cgcccagcgg atgcggcgca
    11341 ccggcagccc gagcgcatgc gcagtcgccg gcgactcgcc gacggcgcgc agcaccagtc
    11401 cggcgcggct gcgctgcagg aagtaccaga ccagcaccgt cagcagcaga cccgtgacga
    11461 tcagcggatc gaagccgaag ccgccgccca gcgggatcag cgccatgcca tcgagcgtgc
    11521 ggctgacgta gtcgcgcccg gcgtaggctg acagcccgct gccgagcatc gtgatcgcca
    11581 gtccgcaggc aacctggctg gcgaggcagt tcagcgtcag ccacgcgaac agcgccgcga
    11641 gtccgacgcc ggcaagcgcc ccggcgagcg ccccggccgc gtagccggcg ccgccgctcg
    11701 cgactgcgaa ggccgtcagc gccccgacca gcatcatgcc ctcgacgccg aggttcagga
    11761 cgccgctctt ctcggcgatc agctcgccga gcgcggcgat cagcagcggt gtggccgctg
    11821 ccagcacgcc ggcgaggatc ggcagccagc ccgacaggct catgaggtgc gcgccgcagg
    11881 cttgcggtac acgcgccgca gccggtaatg cacgaaggtg tcgcaggcga gcagcgcgca
    11941 cagcagtccg ccctggaaca cgccgctgat cgccgccggc agatcgagcg tcatctgcgc
    12001 cgcctcgccg ccgagcgcaa gcagcgacag cagcagcgcc gccagcacca cgccgcgcgg
    12061 atcgagccgg ccgacgaagg cgacgatgat cgccgtgaag ccgtatccgg gcgacaccga
    12121 cggctgcagc tgcccgagcg ggccggcaac ctcggcgtaa ccggcaaaac cggcgcaggc
    12181 cccgcccgtg agcatcgcga tccacaccgc ggcgcgctgc gaatagccgg catgacgcgc
    12241 cgcgagcggt gcctcgccgc tgacctgcag ccggaagccg gcgtaggtgt aacgctgcac
    12301 gaagcagagc agcccggcga ggacgagcgc caccggaaac gccagcgtca cgcgcgcgcc
    12361 ttcgtgcagc ggcgccagca gcgcggcatc cgcgaactgg atcgactgcg gaaagttgaa
    12421 accggccgga tcgcgccacg gaccattgac cagccagccg agcagcagcg gcgcgacgta
    12481 ggccagcatc aggctgctca ggatttcgct gctgccgtag cgcgtgcgca gccaggccgg
    12541 cagcgccgcc cacatcatgc cgccggcgat gccggcgagc gccatcgccg gcgcgatcca
    12601 gagactttca gcgtcgatga agccgtcgca ggcgatcgcg agtccgccgg cgcagatcgc
    12661 cccgagcgtg aactggccgt cggcaccgat gttccagacc ttggcccgga agccgagcgc
    12721 gagcccgagc ccgcagagcg cgagcggtgt tgccttcagc agccattcgc cgatgccgta
    12781 gacactggcg agcggctgca ggaagtacac ggacagcgcc tgcagtggtg catgaccgaa
    12841 ggctgcgaac aacacgctgc cgccgagcag ggtcagcagc agcgccgcga gcggcgcgag
    12901 ccacatcgcc cgcaccgaag gcagcgtccg cgcttcaagc gtgaacatcc cgcatccccg
    12961 gtgctgctgc tgccgtcacc ggcccgaaac cgccccccat ccactgcccg agttcggcgg
    13021 catcggtggc cccggtctcc cggaccggcg acagccgtcc gccggcgagc acggcgatgc
    13081 gatcacagag gccgaacagc tcgtcgaggt cctcggagat caccagcagc gccacgccgt
    13141 cgttgcgaag ccgcagcagc tgttcgtgga tcagcgccgc cgaggcgaca tcgacgcccc
    13201 aggtcggctg cgcgatcacc agcgcctgcg gatgctgcgc cagctcgcgc ccgacgatga
    13261 acttctgcag gttgccgccc gaaagactgt tcgccggcgc ctgcgcgccc ttgcagcgca
    13321 cctgaaagcc ttcgatcagc gcgcgcgcga agcgcagcac cgccggccgg cggatcagcc
    13381 cggcacgcac cagcccgtga ccggcggcgg tgagcagcgt gttgtccgcg agcgagcgcg
    13441 ccggcaccgc cgcgcggccg agccgctcct ccggcaccat gccgaggccg cgcgcgcgcc
    13501 gcgccgccgg tccgagccgg ccgatgtcct cgccggcgag ctgcagcatc gccgcatcgg
    13561 cgagcgccac ctcgccgaac agcgcttcga gcaattcctt ctggccgttg ccggatgccc
    13621 cggcgatgcc gacgatctcg ccgctgtgca cgcaaagctc cagctcccgc agcggcgtgc
    13681 cgaaaggccc gtcggccgtt cgcgacaagc gatcgagcac cagccgcggc gtgcgcggga
    13741 gcgcattcgg cgtcagccgc gtcgccggca gttcgccgcc gatcatcagg cgcgcgaggc
    13801 tcgcggccga ttcttcggcc gggcggcaat gcgcgcggac gcgcccgttg cgcagcaccg
    13861 tggcctggtc acacaggcgg cggatctctt cgagcttgtg gctgatgtag agcaggctca
    13921 cgccctcgcg cgccagccgc cgcagcgtca cgaacagcgc ctcgaccgcc tgcggcgtca
    13981 gcaccgaggt cggctcatcg aggatcagca ggcgtggcga ctgcagcagg cagcgcacca
    14041 gctcgacgcg ctggcgctcg ccgaccgaca gctcgtgcac cggctgcatc ggttcgagcg
    14101 cgaggccgta gtcggccgaa acccggcgga tgcgtccggc cagcgccgcc gggcgtggcg
    14161 catccggcag ggccagcgcg atgttttcgg caacgctcag cgactcgaac agcgagaagt
    14221 gctggaacac catgccgatg ccgagccgcc gtgcctgcgc cggcgaatgg atgcgcaccg
    14281 gctgcccgtc ccagaacagc tcgccggcat cgggcgccac gacgccgtag atgatcttca
    14341 tcagcgtgct cttgccggcg ccattctcgc cgagcacggc gtgaatctcg cccggatgca
    14401 cgacgaggtc gatgtcatcg ttggcgatcg tgccggaata gtgcttgcgg atcgcgcgca
    14461 gttcgaggcg ggggatggtg gcgctcatgc ggtcggcggg gggcgggcga agggcgcgga
    14521 cgggcgcgac cgggcgatgg tagacgcggc cgtgcgctcg cgacagggca atgtgctgcg
    14581 ctgcatcagc aaggaaggcg tcatcggcag gcgttgccgg acgacgcgaa ggccggaggc
    14641 gagcggtggc agggggccgg cgccgccgca cgcggacgcc ggcaacggtc gcagcaggcg
    14701 gacagccgcg atgccgataa atcgcgcgtg cgcagcgatg acacacattc aatccggcat
    14761 gaatcgtgat tcatccgtcc tgacacggaa ttcatgaatc atgacacgcg actcatcgct
    14821 catgaatcgt gattcatcgc tcatgagctg cagttcatcg ctcatgaatc ccaattcacc
    14881 ggtcatgacc ggggcttcat ccatcatgac gcgtgagtca tccgccagga tacgcaagtc
    14941 atcggtcatg acacgtgatt catcgctcat ccatcccgat gcatgggtca tgctgcaggt
    15001 caggccagag ccttgcgcag ttcggtcgct cgttccggag ataaagagaa ctggggagca
    15061 aggggaaaac cacgcatctg cccctgatcg gcatgccatg cgattccggc atcacgccgg
    15121 gcgagtatcg acaggagcgg cggatgtccg gcgactccgg agcggagtgt gtgcggcgca
    15181 gaccacatct gcaagtccgg acatggcatg gcagaagcat aatgccggcg ccggccggat
    15241 ggatggcgat tatttaatgt ccgctgaact tgttgcggag cacttgttga tctagatgct
    15301 tggttgcgtg cgacagcagt ggcagccaca gccacgatcg tgcatgccgc attgcatcac
    15361 gaatcggcct gatggaacga caaggagaaa ctgcatggcc aatatccgtt cgaaggattt
    15421 cctgtccgac atggagcgca agatcaactc catccgccag tttgccagcg atcgcgtgtt
    15481 cggcggcgtc acgctggccg atctggaaag cgcccgcacg gccagcctga atgcgcgtac
    15541 cgacatccag catgctgaaa acgccatgat cgcgctgcgc aatcatcgcc aggaatccga
    15601 tcgccattac gaagaactga tgcgccgcgc cgtcgccggc ctgatcgccg atcccgattt
    15661 cggtcccgac agcgatgcct atgatgcaat gggctacacg cgctacaagg aacgcaagcg
    15721 gcggacgaca aaacccagac gcgaaccgga agattcctga gccggtgccg gacatggcct
    15781 gcggtgcctg ccatcgcagg ccatgcaatc cggatgaatc tgaccctcgc atcgggaatc
    15841 gggcttgtct gcaccgcagt gacgactcgc catgaccatc ggcatcgatg tgtgattggc
    15901 gatttcccga tccatcgatc atccggacgt atctcaacat ctgcagaacg gagtgaaatg
    15961 ccatgaatga tccggtaccg gtcaccgccg aaggtgacga tgaggatcag atcgacggct
    16021 gcgatgtcga ttgcgacggc gaagcgacgg aatacacccc ggacgaggaa ctgcctgcca
    16081 gcgatggcgg cgttgcctga catggcctgg tccctgatct ggttacccga gatcctgaaa
    16141 cgcgccggat tgaaggtggc gctggtggat ggctgggaaa atcgcggtcg cggcgacatg
    16201 gggcgtgtcg aaggcgtgat ctgtcatcac acggtcggtt ctccgaacct gaacatgccg
    16261 agcctgaaag tactgatcaa tggccgtgcg gatctgcccg gtccgctggc acagctcggg
    16321 ctcgggcgtg acggcacgta ttacgtgatc gctgcgggac gctgcaatca tgccggcaaa
    16381 ggtagctgga acggccagac gcacggtaac agcaatttca tcggcatcga ggccgagaac
    16441 accggcaagg cggacgatct gccgtggccg gaggtacaga tggatgccta ccgccgtggt
    16501 gtggcggcga tcctgaagca tgtcggcaag ggcgccgacg gctgcgccgg tcacaaggaa
    16561 tatgcactgc cgaagggacg caagcccgat ccgagtttcg acatgccggc cttccgcaac
    16621 gaggttgccg ccatcctgaa cggcagcgca ccggctccgg tgctgattcc agccttcgaa
    16681 cctgcaactg ccagcacaaa ctccggggct gcccgcccga ctttgcgccg gggtgccagt
    16741 ggtgaactgg tgaaacatct gcagcagaag gtcggtgcca gcgtcgatgg tgatttcggt
    16801 ccgaaaaccg aagctgccgt gcgggtgttc cagcgccaga aaggcatggt gccggatggc
    16861 atcgtcggtc cgaagacctg ggcggcgatg gatgctgtct gagttgcggg cgactgcggt
    16921 gaacgtgatc aggtgaaagc ttggagcgcg cagtttgagg gttcagtatg ttcaccccaa
    16981 cctgcgcgcc cgccggtcgt tctcacccgg cagatgatgc atcgtcttcg tgaacgatga
    17041 gcgcataaat ctcggccacc cgttcagtgg aaatgccggt tctttgctcg atcgctaaag
    17101 gatgatcggt tggctcaagt tcaatgtagg gacggatgcc atcgtcacgc aggtgaatct
    17161 gcgttttcag gtttacggta tcagggtatg gatgtaacca tgcattgagc catccgaaga
    17221 atggaccgat gtgagcgcgt cggggttcgt tgaaatactt cagccagttg aggtagctgg
    17281 cctgactgag ggagacccag actccccagt tgaaagcttc atcctggccg tgaaccggga
    17341 tttcaataca gcctcgtaca aaaaagaagt tgccgtcgat tacgcaatca tcactgccta
    17401 aagcacaacg tgcttcccgc tcttcgacag gaatctggta gtagttcagt ggggcggcgg
    17461 caccaaaact cggcatgcct tcgtgaactt gtccgcaaat gctgcattga aaactgaaag
    17521 acatcgtcga tggcctggtt tgtattgagc gctgcattga ctgatcattc aggcatggga
    17581 ctgcatgacc gttacgagga cggatcaacg atctgcggaa taaacttccg ggtcgtgcag
    17641 ctccatgaat atctccggcc gtttcagggc ggtgaagccg aatttctcat acagtccatg
    17701 cgcatccgca gtcgccagaa tccagcgccg caggccctgc agctgcgggt gagccgtaac
    17761 gcactccagc aaccatccgg ccagtccccg tccccgatac tcgggcagca cgaagacatc
    17821 gccaagatag gcgatcgttg ccgaatcact gatcacacgg gcaaagccga tctgccggcc
    17881 cttgtgcagc agggcgaagc acagtgcgcc ctgaagtgat cttgccacgg tttccctggg
    17941 gatgtttctg gcccaatagg attggctgag gaagccgtgg atgacatcga gatcctgatc
    18001 ttcaggattc gtgctgacga tgtaatctgt tttggaccat tgcattgcgg atgatctgct
    18061 ggtgtcgagg caggtcgatg ataacgtgaa tccggatgag catccggacc cgtcctggct
    18121 gtcgttcagg atcccggttg catcaaaacc ctgcataaac aaggtcttga ttctcatcaa
    18181 ggcatccccg gagcccatcc tccagtatcc cgagcatcgc catcggatat cggaacagga
    18241 gagggccgga tgatgactgc cgccataccc gccatcgacg atcgtccgcc gctgcccggc
    18301 gatctggcga tgtggttctt catcctcgc
//
LOCUS       DAOQIE010000029        18056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1440_length_18056_cov_8.272096, whole genome shotgun sequence.
ACCESSION   DAOQIE010000029 DAOQIE010000000
VERSION     DAOQIE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18056
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1440_length_18056_cov_8.272096"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK144_03330"
     CDS             <1..628
                     /locus_tag="PK144_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF64788.1"
                     /translation="ALLEAALDLGADAILVHHGYFWRGEDARVTGQKRRRLATLLRHD
                     LSLIAYHLPLDAHAEYGNNVALARTLGLRISGALDGGGRTPGLVLHGEPAAPLSARAF
                     AQQIGHALGRVPLHIDAAGDDGPLRRIAWCTGAAAGSIDLAIAAGCDAYVTGEASEPA
                     VHAAREAGVHFYAAGHHATERGGVQALGAHLAAHFGLAFRFVDIPNPV"
     gene            923..1519
                     /gene="petA"
                     /locus_tag="PK144_03335"
     CDS             923..1519
                     /gene="petA"
                     /locus_tag="PK144_03335"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNF64789.1"
                     /translation="MSTADGVNLGRRRFLTATATAVGGVGAAFVAVPFLKSWTPSERA
                     KAAGAPVEADISKLAEGQIMTVEWRGKPVWVVHRSKAMLDALPALDGKMSDPKSEMDQ
                     QPAYAKNEFRSIKPEYLVLVGICTHLGCSPTFRPDLAPADLGAEWKGGFFCPCHGSRF
                     DLAGRVYSGVPAPKNMVVPPYKYLSDTRILVGVGAEAA"
     gene            1519..2769
                     /locus_tag="PK144_03340"
     CDS             1519..2769
                     /locus_tag="PK144_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNF64790.1"
                     /translation="MANHPHPGTTGLLGWVDARFPATKLWREHLSEYYAPKNFNFWYF
                     FGSLALLVLVNQILTGIWLTMSYKPSATEAFASVEYIMRDVDWGWLIRYMHSTGASAF
                     FIVVYLHMFRGLIYGSYKQPRELIWIFGVLIFLALMAEAFMGYLLPWGQMSYWGAQVI
                     INLFGAVPFIGPDLSEWIRGDFVVSDATLNRFFSLHVIAVPLVLVGLVAAHIIALHEV
                     GSNNPDGVEIKKLKDENGIPLDGIPFHPYYSVKDLVGVGFFLIFFCAVIFFLPEMGGY
                     FLEHPNFDPADPLKTPPHIAPVWYFTPFYAILRAVPSMAGSAFPGVVAMGASIVLMFF
                     LPWLDRSPVKSIRYRGPLFKGAVALFVVAFLILGYLGALPTTPGRTLVAQICSAIYFA
                     FFLLMPIYTSIDKTKPVPERVTFK"
     gene            2766..3506
                     /locus_tag="PK144_03345"
     CDS             2766..3506
                     /locus_tag="PK144_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005526845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNF64791.1"
                     /translation="MRKWILAIVLAALPGFALASSEGAALDKANIDPTDKASLQRGAK
                     LFVNYCMGCHSADYQRYNRLARDIGLSDEQVRDNLIFTGAKVGEGMRNAMPKKDAKKW
                     FGAAPPDLTLIARARGVDYLYTYLRTFYIDEARPWGVNNAAFPLVGMPHVLSELQGWQ
                     KARMETHKDAHGNESTVITGYEIVKKGSMSPPEFDSAMRDLVNFLAYVAEPVKLYRQS
                     LGVWVIAFLVLMTAVFYLLKKEYWKDIH"
     gene            3586..4254
                     /locus_tag="PK144_03350"
     CDS             3586..4254
                     /locus_tag="PK144_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNF64792.1"
                     /translation="MAGAASRRSTLTLYSLCNCCHCHRVRLVLLEKGLDPEFVWVNPA
                     SPPPDLLEASPGGHLPTLVDRDVVLYNDRVIIEYLDERYPHPPMLATDPAGRARQRLA
                     LARIEQDLYAQLPRLSRSVREERELAREALRSQLVAGAAVFAARPYFLGTDYSLIDAT
                     LAPLLWRLAHYGIELPPEAAPVLEYAARMFARPAFRQSLSAVEQEMRDDGPAARPPSS
                     ETVR"
     gene            4251..4637
                     /locus_tag="PK144_03355"
     CDS             4251..4637
                     /locus_tag="PK144_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNF64793.1"
                     /translation="MTSNRPYLIRALYEWILDNAMTPHILVNARVEGVDVPPQHVHDG
                     RIVLNVSPSAVHGLAMGNDHIEFSARFGGLARTVRVPVVAVMAIYARENGQGTAFPDE
                     PGSEPPPSPSQPERGSGKRPALKLVK"
     gene            complement(4700..5299)
                     /locus_tag="PK144_03360"
     CDS             complement(4700..5299)
                     /locus_tag="PK144_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNF64794.1"
                     /translation="MIRRTLHLLPVLGLSSLVFLGGPSGCAGVVAGGAAAVSVAHDRR
                     TAGTVVEDESIEMKAVAELDRHGISARSHLNVTSYNRSVLICGEVPDEATRQQAEAVV
                     RRVAEVRQVFNEAAILPASTLASRSNDTWLTTRAKTALFNVDGIRDFDPTRVKVVTER
                     GIVYLMGLVSSAEAESVTAAVRSVPGVQKVVRLFEYIDR"
     gene            complement(5336..5641)
                     /locus_tag="PK144_03365"
     CDS             complement(5336..5641)
                     /locus_tag="PK144_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNF64795.1"
                     /translation="MQQGLKPLMRNFRCRPGEIDLVMHETGTLVFVEVRFRRDCGFGT
                     ALESVTAGKQRRLLRAAAVYLQQHGDTLPCRFDVVGISPGPDGHPVYEWVRDAFRAD"
     gene            5756..6622
                     /gene="rsmI"
                     /locus_tag="PK144_03370"
     CDS             5756..6622
                     /gene="rsmI"
                     /locus_tag="PK144_03370"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNF64796.1"
                     /translation="MSVRIGILYVVATPIGNLLDLSPRAQQTLASVERVAAEDTRHSG
                     ALLRHFGIHTPLVSVHEHNERDRCAGLVGELLAGQSLALVSDAGTPLISDPGYHLVRA
                     AQAAGIEVVAVPGPSSLIAALSVSGLPTDRFVFEGFLPARAAARRARLSELAAETRTL
                     VLFESSHRIAESLADLAETFGPAREAAVARELTKRYESVRRAGLGALAAHFAASEEER
                     RGEFVVLVQGAEAPDAGAGADAGAERVLRLLLAELPLGRAVALAAKITGARRNVLYAR
                     ALELSGDGASGA"
     gene            6662..7018
                     /gene="rnpB"
                     /locus_tag="PK144_03375"
     ncRNA           6662..7018
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK144_03375"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7353..7808
                     /gene="mraZ"
                     /locus_tag="PK144_03380"
     CDS             7353..7808
                     /gene="mraZ"
                     /locus_tag="PK144_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNF64797.1"
                     /translation="MFRGISPLSLDSKGRLSIPARFRDRLSESGSDQVVVTIDRDGCL
                     LLYPLPVWEQVEARLVQLSSTNRAARSLKRLLMGHAEELQPDGSGRLLIPAPLREFAK
                     LEKHVVLVGQGNKFELWDEQTWYRLREQWLAEDGGEAGSLPDDLESLSF"
     gene            7813..8748
                     /gene="rsmH"
                     /locus_tag="PK144_03385"
     CDS             7813..8748
                     /gene="rsmH"
                     /locus_tag="PK144_03385"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNF64798.1"
                     /translation="MTSTLLHQSVLLHEAVDALNVRARGCYLDATFGRGGHARAVLAR
                     LGADGCLLALDRDPEAAAEARALAAIDPRLQFVAGPFSGLAAAVRAHGLEGRLDGILF
                     DLGVSSPQLDDARRGFSFQQDGPLDMRMDPAGGPSAAEWLAAAGEAEISLVLRDLGEE
                     RFHRRIARRVVETRAETPIASTAQLAGLVAAAVPTRERGKHPATRAFQAIRIHVNREL
                     DELQDALAQAVGLLAPGGRLAVISFHSLEDRIVKRFLRAGARGQELPPGLPVTGGPSG
                     ATLKLIGKAVQPSAAEIDANPRARSAVLRVAEKLP"
     gene            8745..9158
                     /gene="ftsL"
                     /locus_tag="PK144_03390"
     CDS             8745..9158
                     /gene="ftsL"
                     /locus_tag="PK144_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNF64799.1"
                     /translation="MNAPLPLLTTEVPVAPVEVAVPPPVAETEKPQAAEAGRLVDEPA
                     EHDPVGQNLGTVLVLVLTILATGVGIVWSKHQSRKLFVELQGLQKVRDDLDIEWNQLQ
                     LEQSTLATESVIDQAARTRLDMGIPAPEAVIYVIR"
     gene            9155..10945
                     /locus_tag="PK144_03395"
     CDS             9155..10945
                     /locus_tag="PK144_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNF64800.1"
                     /translation="MKRSPSSPSRKLRASPGSWTTNRRKLVLGLFALAALGIVARAVY
                     LQLIDTEFLQGQGDERFLRSVEVPAHRGMITDRHGEPLAVSSPVDSVWAHPPTLLANR
                     ERLPELARLLGMTPSELEAKVLSREKKEFMYLRRALEPDAAARIQVRAIPGVFLQREY
                     RRFYPSAEVTSHLLGFTDIDDDGQEGIELAWNSTLQGVPGEKRVIRDRYGHTVDEVES
                     VREPQPGRTVTLSIDRRIQYIAYRAVKAAMLEHGAAAATAVVLDVQTGEVLAMVNQPG
                     TNPNDRGAIRGDLLRNRALTDVYEPGSVMKPFTVAMGLESGRFRPDTPISTSPGHMSV
                     GRYTVRDTHNYGLIDVTRVIVKSSNVGATKIALALPAERLWDLFRKLGFGRTSGIGFP
                     GEQGGWLAHYSRWSTISHANQSFGYGISMTPLQLARAYAALASGGVIRPVSLVKLDRA
                     PEGERILSEATVRKLMPMMEAVVSRDGTAIQASVAGYRVAGKTGTVHKIVGRGYAKNN
                     YYSVFAGFAPVSKPRLAMVVIVDDPKGKSYYGGLVAAPVFSKVMAGALRVMNVPPDNI
                     VDPRLTPARPVMASTTAPAAAPATAKRERP"
     gene            10945..12417
                     /locus_tag="PK144_03400"
     CDS             10945..12417
                     /locus_tag="PK144_03400"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNF64801.1"
                     /translation="MLPLSVLLAGFARDVPELPVTALALDSRTVTPGAVFCAVRGHSG
                     HGLDHVQQAIARGASAVLWEPPAAAPLLPVPAIPIERLGRQVSQLASRLYGIPSHALA
                     VLGVTGTDGKTSCTHFLAQALSEPGRPAGLLGTLGYGLWGGPFESASHTTPDAIGVQQ
                     RLAAFVEAGVRHAAMEVSSHALDQGRVEAVHYACAILTHLSRDHLDYHGTAAAYAAAK
                     MRLFAELDIGAAVLNLDDAFGRSLCTVARAPIHGYGLDVLDTEGLAGWVQGSEPVCDT
                     DGLRLSVHTHLGAAELASTLLGRFNAQNLLACLAALLALGMPLDEAVGRLARSRTVPG
                     RMERFGGGADRPLAVVDYAHTPNALQSVLTALRAHTAGRLYCVFGCGGDRDAGKRPLM
                     GAAAERLADRVILTSDNPRSEDPLAILADIRAGCAEPARVQVEPERAAAIRQALAAAR
                     PGDVVLIAGKGHEDYQIIGSERRVYSDRAEVAALFGETSA"
     gene            12414..13787
                     /gene="murF"
                     /locus_tag="PK144_03405"
     CDS             12414..13787
                     /gene="murF"
                     /locus_tag="PK144_03405"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNF64802.1"
                     /translation="MKPQPLSHLATGLCAELTGHDTEVRGVSTDSRTIAPGELFVALA
                     GPNFDGHAWVATAAARGACAALVAHPQPLDLPQLLVPDTRLALGRLGALARAAFRGPV
                     IGLTGSNGKTTLKEMSAAILRRRARVLATRGNLNNDIGVPLTLLGLDEDHDCAVIEMG
                     ANHPGEIAYLTALVQPSVAILNNAGPCHLEGFGSLDGVARAKGEIFTGLGAQGVAILN
                     ADDRYFGYWRELVGARPALSFGLDAPADVSAVVLDAATQRVRLSLPQREHIEFELPLS
                     GRHNLRNACAAAAATYALGAGAEDIAAGLASLHPVAGRLQRLAGLHGATLVNDAYNAN
                     PASLAAGLETLAAEPGEHWLVLGDMGELGAEADALHAQSGERARACGFARLYALGSRS
                     RHAVRAFGAGGRHFDDIDSLTATLAADLAAGVAPTILVKGSRSMRMERVVRALDPHDH
                     DPLAGAH"
     gene            13792..14874
                     /gene="mraY"
                     /locus_tag="PK144_03410"
     CDS             13792..14874
                     /gene="mraY"
                     /locus_tag="PK144_03410"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNF64803.1"
                     /translation="MLVFLAELLTQLYSGFNVFSYLTLRAILGLLTALLLALLVGPVM
                     IRRLSQYQIGQQVRSDGPQTHLKKAGTPTMGGALILVAIAVSTLLWADLRNPYVWIVL
                     GVTLAFGAIGWVDDYRKLVKKNSKGLSAKEKYFWQTIAGALAALLLYLIAKTPVSTQL
                     IVPFFKHVAIDLGPWFLLLAYLTIVGSSNAVNLTDGLDGLAIMPTVMVAGGLGVIAYV
                     TGNSVFAGYLGIPYISGVGEMIVFCGAIFGAGLGFLWFNAYPAQVFMGDVGALALGAA
                     LGIVAVIVRQEIVLVIMGGVFVMETVSVILQVGSYKLTGRRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIITVILVMIGLSTLKIR"
     gene            14888..16273
                     /gene="murD"
                     /locus_tag="PK144_03415"
     CDS             14888..16273
                     /gene="murD"
                     /locus_tag="PK144_03415"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNF64804.1"
                     /translation="MVPAPRPDLHGLTLVVGLGVSGLSAARALAHLGASFAVVDTRSA
                     PPGLAELDRIAPEAEVCCGDFGLIQAEALFARAARLIVSPGISVRTPAIAACAARGVP
                     VWGDIELFARLTDVPVIGITGSNGKSTVTTLLGEMATAAGVRPAVGGNLGTAALDLWL
                     EREAAGDVPGLYVLELSSFQLETTHSLACVAATVLNVSADHLDRYDGMDDYAAAKARL
                     FERAAVQVVNRDDARVRAMIRPERPVIGFGSDRPPAAGDYGLERHGDTIRLMHGATAL
                     LDAAELRIGGLHNALNVLAALALGAAAGLPLAPMLAAARAFAGLAHRTQLVAEHAGVR
                     WYDDSKGTNVGATVAAIAGLPGRLVLIAGGDGKGQDFAPLAEALDGRARAVVLLGRDA
                     PRIEAALAGRLPCERVADMEQAVARAAALAQPGDIVLLSPACASLDMYRSYAERGRLF
                     AQAARRVAGSC"
     gene            16267..17472
                     /gene="ftsW"
                     /locus_tag="PK144_03420"
     CDS             16267..17472
                     /gene="ftsW"
                     /locus_tag="PK144_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNF64805.1"
                     /translation="MLNVVIDRVRHVAPDHDRLAPLDPWLVFPAIGLIAFGVLMVTSA
                     SMPIADRQADAPLYFLYREFAYVALGLLLGWLAFQVEVETWYRRGPAILMVVGVLLVL
                     VLIPGIGRRVNGSWRWINLGIMNVQVSELAKLASILYVAGYLRRHAQALRESIVAMIR
                     PLMVLGVLALLLLMEPDFGATMVIIAVALGLVFLGGVNFWQFSAMQLLVVGGMAVLLW
                     SSPYRRARLSSFLNPWEDPFNKGFQLTQSLIAIGRGEFDGVGLGASVQKLFYLPEAYT
                     DFIFAIIAEELGLLGVLCVLATYLVIVVRSFQVGARCERLRQPFGASVAYGIGLWIGF
                     QALINMGVNMGVLPTKGLTLPFISYGGSSLVVMCIAYAILLRVDAESRLEEDPKLRRD
                     RLLAKVRAP"
     gene            17469..>18056
                     /locus_tag="PK144_03425"
     CDS             17469..>18056
                     /locus_tag="PK144_03425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF64806.1"
                     /translation="MSTPVPMPAGGAPVLVMAGGTGGHVFPALAVAAELRARGVPVVW
                     LGARSGLEARLVPAHGFAVEWLEVAGLRGKGWARRLAAPLMLARALWQAARVLRRLRP
                     RLVLGFGGYVSGPAGLMARLLGVPLLIHEQNAIAGYTNRILARLATRVCEAFPGAFPA
                     ALGAVTTGNPVRAAIAQLPSPVVRGATRGGRPRLLV"
BASE COUNT         2540 a         6408 c         6256 g         2852 t
ORIGIN      
        1 ggcgctgctc gaagcggcgc tcgatctcgg tgccgatgcg atcctcgtgc atcacggcta
       61 cttctggcgc ggcgaggatg cgcgcgtcac cggtcagaag cgccggcgcc tggcgacgct
      121 gctgcgccac gatctctcgc tgatcgccta ccacctgccg ctcgatgcgc acgccgagta
      181 cggcaacaac gtcgcgctcg cccgcacgct cggcctgcgc atcagcgggg cgctcgacgg
      241 cggcggacgc acgccggggc tggtcctgca cggcgagccg gccgcgccgc tgtcggcgcg
      301 cgcgttcgcg cagcagatcg ggcatgcgct cgggcgcgtg ccgctgcaca tcgacgccgc
      361 cggcgacgac gggccgctgc ggcgcatcgc ctggtgcacc ggcgcggccg ccggctcgat
      421 cgacctggcg atcgccgccg gctgcgatgc ctacgtcacc ggcgaggcct cggagccggc
      481 cgtgcatgcg gcgcgcgagg ccggcgtcca cttctatgcg gccggacacc atgccaccga
      541 gcgcggtggc gtgcaggcgc tcggggcgca tctggcggcg catttcggcc tcgcgttccg
      601 cttcgtcgac atcccgaatc cggtctgacc ttcgcggaat cttccccgga tcgttcctga
      661 atctttcccg gatctttcct gaatcccgcc ttgcagaagt ggcggcaaca gccacgctgt
      721 gcacgcccgc aacccttgat tttcctcaag tgaaataagg gtttcgtctt gattcttacg
      781 aaacgttata cgaacatccc gcgtttagtt gcggccggcg gttcagctcg cgggcggctg
      841 ccgccgtggc ctccgcagac cggtcgggac gctcgataaa ccggtcaaaa tcaatttttt
      901 tcagtctgtt aggagagagc gtatgagtac ggcagacgga gtgaacctcg gcaggcggcg
      961 gttcctgacc gccactgcca ccgccgtcgg tggcgtcggc gcagcgttcg tggcggtgcc
     1021 cttcctgaag tcctggacac cgagcgaacg ggccaaggcg gcgggcgcgc cggtcgaggc
     1081 cgacatcagc aagctcgccg aaggccagat catgacggtc gaatggcgcg gcaagccggt
     1141 gtgggtcgtg catcgcagca aggccatgct cgatgcgctg cccgcgctcg acggcaagat
     1201 gtccgatccg aagtccgaga tggaccagca gccggcctac gccaagaacg agttccgctc
     1261 gatcaagccc gaatatctgg tgctggtcgg catctgcacg cacctcggct gctcgccgac
     1321 cttccggccc gacctcgcac ccgccgacct cggtgccgaa tggaagggcg gcttcttctg
     1381 tccgtgccac ggttcccgct tcgacctcgc cgggcgcgtc tacagcggcg tgccggcgcc
     1441 gaagaacatg gtcgttcccc cgtacaagta cctgagcgac acccgcattc tggtcggcgt
     1501 cggcgcggag gctgcgtaat ggcgaatcat ccacatccgg gtacgaccgg cctgctcggc
     1561 tgggtcgatg cgcgctttcc ggcgaccaag ctctggcgcg agcacctgtc cgagtattac
     1621 gcgccgaaga acttcaactt ctggtacttc ttcggctcgc tggcgctgct ggtgctggtc
     1681 aaccagatcc tgaccggcat ctggctgacg atgagctaca agccctcggc gaccgaagcc
     1741 ttcgcgtccg tcgaatacat catgcgcgac gtcgactggg gctggctgat ccgctacatg
     1801 cactcgaccg gtgcatcggc gttcttcatc gtcgtctacc tgcacatgtt ccgcggcctg
     1861 atctacggct cgtacaagca gccgcgcgaa ctgatctgga tcttcggcgt gctgatcttc
     1921 ctcgcgctga tggccgaggc cttcatgggc tacctgctgc cctggggcca gatgtcgtac
     1981 tggggtgcgc aggtcatcat caacctgttc ggcgccgtgc ccttcatcgg cccggatctc
     2041 tccgaatgga tccgcggtga cttcgtcgtc tccgacgcga cgctgaaccg cttcttctcg
     2101 ctgcacgtga tcgcggtgcc gctggtgctg gtcggcctgg tcgccgcgca catcatcgcg
     2161 ctgcacgaag tcggctcgaa caaccccgat ggcgtcgaga tcaagaagct caaggacgag
     2221 aacggcatcc cgctcgacgg catcccgttc cacccgtact actcggtcaa ggatctggtc
     2281 ggcgtcggct tcttcctgat cttcttctgc gccgtgatct tcttcctgcc ggagatgggc
     2341 ggctacttcc tggagcaccc gaacttcgat ccggccgatc cgctgaagac cccgccgcac
     2401 atcgcgccgg tgtggtactt cacgcccttc tacgcaatcc tgcgcgccgt gccgtcgatg
     2461 gccggctcgg ccttcccggg cgtggtcgcg atgggcgcct cgatcgtgct gatgttcttc
     2521 ctgccctggc tcgaccgcag cccggtcaag tcgatccgct accgcggccc gctgttcaag
     2581 ggcgccgtcg cgctgttcgt cgtcgccttc ctgatcctcg gctacctcgg cgcgctgccg
     2641 accacgccgg gccgcacgct ggtcgcgcag atctgctcgg cgatctactt cgcgttcttc
     2701 ctgctgatgc cgatctacac gtcgatcgac aagaccaagc cggttccgga aagggtgacg
     2761 ttcaaatgag aaagtggatt ctggccatcg tcctcgccgc gctgcccggc ttcgcactgg
     2821 cctccagcga aggcgccgca ctcgacaagg cgaacatcga cccgaccgac aaggcctcgc
     2881 tgcagcgcgg tgcgaagctg ttcgtcaatt actgcatggg ctgccactcg gcggactacc
     2941 agcgctacaa ccggctggcg cgcgacatcg gcctgagcga cgagcaggtc agggacaacc
     3001 tgatcttcac cggtgccaag gtcggcgaag gcatgcgcaa cgccatgccc aagaaggacg
     3061 cgaagaagtg gttcggtgcg gcgccgcccg acctgaccct gatcgcgcgc gcgcgcgggg
     3121 tggactacct gtacacctat ctgcgcacgt tctacatcga cgaggcgcgg ccctggggcg
     3181 tcaacaacgc cgccttcccg ctggtcggca tgccgcacgt gctgagcgaa ctgcagggct
     3241 ggcagaaggc caggatggaa acgcacaagg atgcccacgg caacgagtcg acggtcatca
     3301 ccggctacga gatcgtcaag aagggcagca tgagtccgcc ggagttcgac tcggcgatgc
     3361 gtgacctggt caacttcctc gcctacgtcg ccgaaccggt caagctgtac cggcagtcgc
     3421 tcggcgtgtg ggtcatcgcg tttctggtcc tgatgacggc ggtcttctac cttctgaaga
     3481 aggaatactg gaaggacatc cactgacgca cggcgcgcgg ggcggattgc cggtcatggc
     3541 agccgccccg cgtcccgcat cccgccgatc tatccggaga gggccatggc cggagcagcc
     3601 agccgcagat cgaccctgac gctgtattcg ctctgcaact gctgccactg ccaccgcgtg
     3661 cgtctggtgc tgctggaaaa ggggctggac ccggagttcg tctgggtcaa ccccgccagc
     3721 ccgccccccg acctgctcga agccagtccc ggcggccatc tgccgacgct ggtcgatcgc
     3781 gatgtcgtgc tctacaacga ccgcgtcatc atcgaatacc tcgacgagcg ctatccgcat
     3841 ccgcccatgc tcgcgaccga cccggccggt cgtgcccgcc agcggctggc gctcgcccgc
     3901 atcgaacagg atctgtacgc gcagctgccg cggctgtcgc gcagcgtgcg cgaggagcgc
     3961 gagctggcgc gcgaggcgct gcgttcgcag ctcgtggccg gcgccgcggt gttcgcggcg
     4021 cgcccgtact tcctcggcac cgactacagc ctgatcgacg cgacgctggc gccgctgctg
     4081 tggcgcctcg cgcactacgg catcgaactg ccgcccgagg cggcgccggt gctcgaatac
     4141 gcggcgcgca tgttcgcgcg gccggcgttt cgccagagtc tgagtgcggt cgagcaggaa
     4201 atgcgcgacg atggtcccgc cgcccgcccc ccgtccagcg agaccgtgcg atgacctcca
     4261 atcgccccta tctgatccgg gccttgtacg aatggatcct cgacaacgcc atgaccccgc
     4321 acatcctcgt caacgccagg gtcgaggggg tcgacgtgcc gccgcagcac gtgcatgacg
     4381 gtcgcatcgt gctgaacgtc agcccgagcg ccgtgcatgg cctggcgatg ggcaacgacc
     4441 acatcgagtt cagtgcccgc ttcggcgggc tggcgcgcac cgtgcgcgtg ccggtggtgg
     4501 cggtgatggc gatctacgcg cgcgagaacg gtcagggcac ggccttcccg gacgagccgg
     4561 gcagcgaacc gccgccgtcg ccgtcccagc ccgagcgtgg cagcggcaag cgcccggcgc
     4621 tgaagctcgt caagtaattc gtcaagcaat tccgggcagg caggcgcccg ttcagccgcg
     4681 cgcccggttc ccgggcggat cagcggtcga tgtactcgaa cagccgcacg accttctgca
     4741 cgcccggcac gctgcgcacc gccgccgtga ccgactcggc ctcggcgctg ctcaccagcc
     4801 ccatcagata gacgatgccg cgttcggtca cgaccttgac ccgggtcggg tcgaagtcgc
     4861 ggatgccgtc gacgttgaac agcgccgtct tcgcgcgcgt cgtcagccag gtgtcgttcg
     4921 agcggctggc gagcgtgctc gccggcagga tggcagcctc gttgaacacc tgccgcacct
     4981 cggcgacgcg gcgcacgacg gcctcggcct gctggcgcgt ggcctcgtcg ggcacctcgc
     5041 cgcagatcag cacgctgcgg ttgtagctcg tgacgttcag gtgcgagcgg gcactgatgc
     5101 cgtgacggtc gagctcggcg accgctttca tctcgatgct ctcgtcctcg acgacggtgc
     5161 cggccgtgcg ccggtcgtgc gcgacgctga ccgcggccgc gccaccggcg acgacgccgg
     5221 cgcagccgct cgggccgccg aggaagacaa ggctgctcag gccgagcacg gggagcaggt
     5281 gcagggttcg gcgaatcatg tgtgcgggat tccgtaggca ggcgggtacg cggtttcagt
     5341 cggcgcgaaa ggcgtcgcgt acccattcgt aaacgggatg gccgtccggg ccgggcgaga
     5401 tgccgaccac gtcgaagcgg cacggcagcg tatcgccgtg ctgttgcagg tagactgccg
     5461 cggcccgcag caggcggcgc tgcttgccgg ccgtgacgct ttcgagcgcc gtgccgaagc
     5521 cgcaatcgcg gcggaagcgc acctcgacga acacgagggt gccggtctcg tgcatcacga
     5581 gatcgatctc gccgggccgg cagcggaaat tgcgcatcaa cggtttcagg ccctgctgca
     5641 ccagccaggc ttcggccaag gcctcgccgg ccgcgccgcg cagcagatgg gcaggccggc
     5701 cggcgccgga ggtcatggcg ccggagccct gccacacatc ttccggagtc ctgtcgtgtc
     5761 cgtacgcatc ggcatcttat acgtggtggc cacgccgatc ggcaatctgc tcgatctgag
     5821 tccgcgcgcg cagcagacgc tcgcgtcggt cgagcgggtg gcggccgagg atacccgcca
     5881 cagcggtgcg ctgctgcgcc atttcggcat ccatacgccg ctggtgtcgg tgcacgagca
     5941 caacgagcgc gaccgctgcg ccggactggt cggggagctg ctcgccggcc agtcgctggc
     6001 gctggtgtcc gatgccggca cgccgctgat ttccgatccg ggctatcacc tcgtgcgtgc
     6061 cgcgcaggcg gccggcatcg aggtggtcgc cgtgcccggt ccgagcagcc tgatcgctgc
     6121 gttgtcggtc tcggggctgc cgaccgatcg cttcgtgttc gaaggtttcc tgccggcccg
     6181 agccgcggcc cgccgtgcac ggctgtcgga actggcggcc gagacgcgca cgctggtgct
     6241 gttcgagtcc agccaccgca tcgccgagag cctcgccgat ctcgccgaaa ccttcgggcc
     6301 ggcgcgcgag gcggccgtcg cgcgcgagct gaccaaacgc tacgaatccg tgcgccgggc
     6361 cgggctcggg gcgctggccg cgcatttcgc agcgagcgag gaggagcgcc gcggcgagtt
     6421 cgtcgtgctc gtgcagggcg ccgaggcgcc cgatgccggg gccggagcgg atgccggggc
     6481 cgagcgcgtg ctgcgcctgc tgctcgccga gctgccgctc gggcgcgccg tggcgctggc
     6541 ggcgaagatc accggtgccc gcaggaacgt gctgtacgca cgggcgctgg agctgtccgg
     6601 cgacggggca tccggcgcat gacttgccct gccgtcggtg gccgcgcaga atccgcgccc
     6661 ggaagtcggc cggacgatcg cggcccccgc gtgcgggggc ggaggaaagt ccgggctccg
     6721 cagggcaggg tgccaggtaa cgcctggggg gcgtgagccc acggaaagtg ccacagaaaa
     6781 caaaccgccc aagtcgccgc gaggcgaacg gcaagggtga aaaggtgcgg taagagcgca
     6841 ccgcggcgct ggcaacagtc gccggcacgg aaaaccccac ccggagcaag accaaatagg
     6901 ggagcgccag cgtgtggccc gcacggctcc cgggtaggtc gctacaggcg tccggtgacg
     6961 ggcgtcgcag atgaatgatc gtcctcgaca gaacccggct tacaggccga cttcctcctt
     7021 cctgaatctc cctgtttcca gccccggctt gcggttcgcc gcaagccgcg cggctgtctg
     7081 tccgtgcggc gggggacatg acgttttccg gcggtggctg acagcggttt gctcctgaat
     7141 ggtgcaatgc ggcatcggca gtccggtggc tgttgtgggg gcgcaaccca tgttgtatgc
     7201 gcgccgataa gctgttgtag aaaaagcaaa accgtggcgt ccgcgggccg tgttctcctt
     7261 gacaggcttc cggcgcggtt cctatagtgg gaaaacgtgg aacgtagtgg gacagtcggg
     7321 caggccgcgg tacggcagcg aggatcaggc gcttgtttcg cgggatttcc cctctttcgc
     7381 tcgacagcaa gggacggctc tcgatccccg cccggttccg ggatcggctc tccgaaagtg
     7441 gcagtgacca ggtggtcgtc acgatcgatc gtgacgggtg tctccttttg tatccgctgc
     7501 cggtctggga gcaggtggaa gcgcgactgg tgcagctgtc gagtacgaac cgcgccgccc
     7561 gcagcctgaa gcggttgctg atggggcacg ccgaggaact gcaacccgac ggctcggggc
     7621 gtctgctgat accggcgccg ctgcgggaat tcgcgaagct cgaaaaacat gtggtgctgg
     7681 tggggcaggg gaacaagttc gagctctggg acgagcagac ctggtatcgg ctgcgcgagc
     7741 agtggctggc cgaggacggc ggcgaggcag gctccctgcc cgacgatctg gaatcgctct
     7801 ccttctgaag tcatgacctc caccctgctt catcaaagcg tgctgctgca cgaggccgtc
     7861 gatgccctga acgtgcgggc acggggctgc tatctcgatg ccaccttcgg ccgcggcggt
     7921 cacgcccgcg cggtgctggc gcgcctcggg gccgacggct gcctgctggc cctcgatcgc
     7981 gatccggaag cggccgccga ggcgcgtgcg ctcgcggcca tcgatccccg cctgcagttc
     8041 gtggccggtc cgttcagcgg gctggcggcg gccgtgcggg cgcacggtct cgaaggccgg
     8101 ctcgacggca tcctgttcga tctcggcgtc tcgtcgccgc aactcgacga tgcgcgccgc
     8161 ggcttcagct tccagcagga cgggccgctc gacatgcgca tggatccggc cggcgggccg
     8221 agtgcagccg aatggctggc ggcggccggc gaggcggaga tttccctggt gctgcgcgat
     8281 ctcggcgagg agcgttttca ccggcgcatc gcccggcgcg tcgtcgagac ccgcgccgaa
     8341 accccgatcg ccagcaccgc gcagcttgcc ggcctcgtgg ccgccgccgt gccgacgcgc
     8401 gagcgcggca agcatccggc gacgcgcgcc ttccaggcga tccgcatcca cgtcaaccgc
     8461 gaactcgacg aactgcagga cgcgctcgcg caggccgtcg ggctgctcgc gcccggcggc
     8521 cggctcgccg tgatcagctt ccactcgctg gaagaccgca tcgtcaagcg cttcctgcgc
     8581 gccggcgcgc gcggccagga actgccgccc gggctgccgg tgaccggcgg gccgagcggc
     8641 gcgacgctga agctcatcgg caaggccgtg cagccgtccg cggccgagat cgacgccaat
     8701 ccgcgcgcgc gcagcgccgt gctgcgcgtc gccgagaagc tgccatgaac gcgccgctgc
     8761 cgctcctgac gaccgaggtc ccggtcgctc ccgtcgaagt cgcggtgccg ccgccggtcg
     8821 ccgagaccga aaaaccgcag gctgccgagg ccgggcgact cgtcgacgag ccggccgagc
     8881 acgacccggt cggccagaac ctcggcacgg tgctggtgct ggtgctgacg atcctcgcca
     8941 ccggtgtcgg catcgtctgg agcaagcacc agagccgcaa gctgttcgtc gagctgcagg
     9001 ggctgcagaa ggtccgcgac gacctcgaca tcgaatggaa ccagctgcag ctggaacaga
     9061 gcacgctcgc gaccgagtcg gtgatcgacc aggccgcgcg cacccggctg gacatgggca
     9121 tacccgcgcc cgaagcggtc atctacgtga tccgatgaaa cgctctccgt catcgccgtc
     9181 gcgcaagctg cgcgcgtccc cgggcagctg gacgaccaat cgccgcaagc tcgtcctcgg
     9241 cctgttcgcg ctggccgcgc tcggcatcgt cgcgcgcgcc gtctacctgc agctgatcga
     9301 taccgaattc ctgcaggggc agggcgacga gcgcttcctg cgcagcgtcg aggtgccggc
     9361 acaccgcggc atgatcaccg accgccacgg cgagccgctg gcggtcagct cgccggtcga
     9421 cagcgtctgg gcgcatccgc cgacgctgct cgccaaccgc gagcgcctgc cggagctcgc
     9481 acgcctgctc ggcatgaccc cctcggaact cgaagccaag gtcctgtcgc gcgagaagaa
     9541 ggaattcatg tacctgcgtc gcgcgctcga acccgatgcc gccgcgcgca tccaggtgcg
     9601 ggcgattccg ggcgtgttcc tgcagcgcga ataccggcgc ttctatccgt cggccgaggt
     9661 gacctcgcac ctgctcggct tcaccgacat cgacgacgac ggtcaggaag gcatcgagct
     9721 ggcgtggaac tcgacgctgc agggcgtgcc cggcgagaag cgcgtgatcc gtgaccgcta
     9781 cggccacacc gtcgacgagg tcgagagcgt gcgcgagccg cagcccgggc gcacggtgac
     9841 gctgtcgatc gaccggcgca tccagtacat cgcctaccgc gcggtcaagg ccgcgatgct
     9901 cgaacacggc gctgccgccg ccactgccgt cgtgctcgac gtgcagaccg gcgaggtgct
     9961 ggcgatggtc aaccagcccg gcaccaaccc gaacgatcgc ggcgcgatcc gcggcgacct
    10021 gctgcgcaac cgcgcgctga ccgacgttta cgaacccggc tcggtcatga agccgttcac
    10081 ggtggcgatg ggcctcgaaa gcggcaggtt ccggccggac acgccgatca gcaccagccc
    10141 cggccacatg agcgtcgggc gctacacggt gcgcgacacg cacaactacg gcctgatcga
    10201 cgtgacgcgc gtgatcgtca agtcgagcaa cgtcggcgcc accaagatcg cgctggcgct
    10261 gccggccgag cggctgtggg acctgttccg caagctcggc ttcgggcgca ccagcggcat
    10321 cggcttcccg ggcgagcagg gcgggtggct cgcgcactac tcgcgctgga gcacgatcag
    10381 ccacgccaac cagagcttcg gctacggcat ctcgatgacg ccgctgcagc tcgcgcgggc
    10441 ctacgcggca ctcgcctccg gcggcgtgat ccggccggtg tcgctggtca agctcgaccg
    10501 ggcgccggag ggcgagcgca ttttgtccga ggccacggtc aggaagctga tgccgatgat
    10561 ggaagccgtc gtcagccgcg acggcacggc gatccaggcg agcgtcgccg gctaccgcgt
    10621 cgccggcaag accggcaccg tccacaagat cgtcggccgc ggctacgcga agaacaacta
    10681 ctactcggtg ttcgcgggct tcgcgccggt cagcaagccg cggctggcga tggtcgtcat
    10741 cgtcgatgac ccgaagggca agtcctacta cggcgggctg gtcgccgcgc cggtgttcag
    10801 caaggtcatg gccggagcgc tgcgcgtcat gaacgtgccg ccggacaaca tcgtcgatcc
    10861 gcgcctgacg ccggcgcggc cggtgatggc aagcacgacg gcaccggctg ccgcgccggc
    10921 gacggcgaaa cgggagcgtc cgtgatgctg ccgctgtccg tcctgctcgc cggattcgcc
    10981 cgcgacgtgc ctgaactgcc ggtcacggcg ctcgcgctcg acagccgcac cgtgaccccg
    11041 ggggcggtgt tctgcgccgt gcgcggccac agcggccacg gtctcgacca cgtgcagcag
    11101 gcgatcgcgc gcggtgcgag tgccgtgctg tgggagccgc cggccgcggc accgctgctg
    11161 ccggtgccgg cgatcccgat cgaacggctc ggccggcagg tgtcacagct ggcgagccgg
    11221 ctgtacggca tcccgtcgca cgcgctggcg gtgctcggcg tcaccggcac cgatggcaag
    11281 acctcgtgca cgcacttcct cgcgcaggcg ctttccgaac ccggacgccc ggcgggcctg
    11341 ctcggcacgc tcggctacgg cctctggggc gggccgttcg agagcgcctc gcacacgacc
    11401 cccgatgcga tcggcgtgca gcagcgcctc gccgcctttg tcgaggccgg cgtgcgtcac
    11461 gcggccatgg aagtctcctc gcacgcgctc gatcaggggc gggtcgaggc cgtgcactac
    11521 gcctgcgcga tcctgacgca cctgagccgc gatcacctcg actatcacgg cacggccgcg
    11581 gcctatgcgg cggccaagat gcggctgttc gccgaactcg acatcggcgc cgcggtgctg
    11641 aacctcgatg acgccttcgg gcgttcgctg tgcacggtgg cgcgcgcgcc gatccacggc
    11701 tacggcctcg atgtgctcga caccgagggg ctggccggct gggtgcaggg cagcgagccg
    11761 gtctgcgaca ccgacggcct gcgcctgagc gtgcacaccc acctcggggc cgccgaactc
    11821 gcgagcacgc tgctcgggcg cttcaacgcc cagaacctgc tcgcctgcct cgcggcgctg
    11881 ctcgcgctcg gcatgccgct cgatgaggcc gtcggccggc ttgcgcgctc gcgcacggtg
    11941 ccggggcgca tggagcgctt cggcggcggt gcggaccgcc cgctcgcggt cgtcgactac
    12001 gcccacacgc cgaacgcgct gcagtcggtg ctgaccgcgc tgcgcgcaca cacggccgga
    12061 cggctgtact gcgtgttcgg ctgcggcggc gatcgcgatg ccggcaagcg tccgctgatg
    12121 ggcgcggccg ccgagcgcct cgccgatcgc gtgatcctga ccagcgacaa tccgcgcagc
    12181 gaggacccgc tggcgatcct cgccgacatc cgggccggct gcgccgagcc cgcgcgcgtg
    12241 caggtcgagc ccgagcgcgc cgcggccatc cgccaggcgc tggcggcggc ccgccccggg
    12301 gacgtggtgc tgatcgccgg caagggccac gaggattacc agatcatcgg cagcgaacgg
    12361 cgcgtgtaca gcgaccgcgc cgaggtcgcc gccctgttcg gggagacatc tgcatgaagc
    12421 cgcagccgct ctcgcacctc gcgaccggac tgtgcgccga actgaccggc cacgacaccg
    12481 aagtccgcgg cgtgtcgacc gattcgcgca cgatcgcgcc cggcgagctg ttcgtcgcgc
    12541 tggccggccc gaacttcgac ggtcacgcct gggtcgcgac cgcggccgcg cgcggggcct
    12601 gcgcggcgct ggtcgcgcat ccgcagccgc tcgacctgcc gcagctgctg gtgccggaca
    12661 cccggctcgc gctcgggcgg ctcggtgcgc tggcgcgcgc ggccttccgc ggtccggtga
    12721 tcggcctgac cggctccaac ggcaagacca cgctgaagga gatgtcggcg gcgatcctgc
    12781 gccggcgcgc ccgggtgctg gcgacgcgcg gcaacctgaa caacgacatc ggcgtgccgc
    12841 tgacgctgct cggcctcgat gaggatcacg actgcgcggt catcgagatg ggtgccaacc
    12901 accccggcga gatcgcctac ctgaccgccc tcgtgcagcc ctcggtcgcg atcctcaaca
    12961 acgccggtcc ctgccacctc gaaggcttcg gcagcctcga cggcgtcgcg cgcgccaagg
    13021 gcgagatctt caccgggctc ggtgcgcagg gcgtggcgat cctcaacgcc gatgaccgct
    13081 acttcggcta ctggcgcgag ctggtcggcg cccgcccggc gctgagcttc gggctggacg
    13141 caccggccga cgtgagcgcc gtcgtcctcg atgccgccac gcagcgcgtg cgcctgagcc
    13201 tgccgcagcg ggagcacatc gagttcgaac tgccgctgag cggccggcac aacctgcgca
    13261 acgcctgcgc ggccgccgcc gcgacctatg cgctcggcgc gggcgccgaa gacatcgccg
    13321 ccggccttgc gtcgctgcat ccggtcgccg ggcgcctgca gcgcctcgcc ggcctgcacg
    13381 gcgcgacgct cgtcaacgac gcctacaacg ccaatccggc ctcgctcgcc gccgggctgg
    13441 agacgctggc ggccgagccc ggcgagcact ggctggtgct cggcgacatg ggcgagctcg
    13501 gggccgaggc cgacgcgctg cacgcccaga gcggcgagcg cgcgcgtgcc tgcggcttcg
    13561 cgcgcctgta cgcgctgggc agccgcagcc ggcacgcggt gcgggccttc ggtgccggtg
    13621 gccggcactt cgacgacatc gacagcctga cggcgacgct cgcggcggat ctcgccgccg
    13681 gcgtggcgcc gacgattctg gtcaagggct cgcgttcgat gcgcatggag cgcgtcgtgc
    13741 gggccctcga tccccatgac cacgaccccc tggcgggagc gcactgaaca aatgctggtt
    13801 ttcctggccg agctgctgac ccagctgtac agcggcttca atgtcttttc gtacctgacg
    13861 ctgcgcgcga tcctcgggct gctgacggcg ctgctgctcg cgctgctggt cgggccggtg
    13921 atgatccgcc ggctgtcgca ataccagatc ggccagcagg tgcgcagcga cgggccgcag
    13981 acgcacctga agaaggccgg cacgccgacg atgggcggcg cgctgatcct ggtcgcgatc
    14041 gcggtctcga cgctgctgtg ggcggacctg cgcaatccct acgtgtggat cgtgctcggc
    14101 gtgacgctgg ccttcggcgc catcggctgg gtcgatgact accgcaagct ggtcaagaag
    14161 aacagcaagg gtctgtcggc gaaggagaaa tacttctggc agaccatcgc cggcgcgctc
    14221 gccgcgctgc tgctgtacct gatcgccaag acgccggtgt cgacgcagct gatcgtgccg
    14281 ttcttcaagc acgtcgcgat cgacctcggt ccgtggttcc tgctgctcgc atacctgacc
    14341 atcgtcggct ccagcaacgc cgtgaacctg accgacggcc tcgacggtct ggcgatcatg
    14401 ccgacggtca tggtcgccgg cggcctcggc gtcatcgcct acgtgaccgg caactcggtg
    14461 ttcgccggct atctcggcat cccgtacatc tccggcgtcg gcgagatgat cgtgttctgc
    14521 ggcgcgatct tcggcgccgg cctcggcttc ctgtggttca acgcctatcc ggcgcaggtg
    14581 ttcatgggcg atgtcggcgc gctcgcgctc ggcgcggccc tcggcatcgt cgccgtgatc
    14641 gtgcgtcagg agatcgtgct ggtgatcatg ggtggcgtgt tcgtcatgga gaccgtgtcg
    14701 gtgatcctgc aggtcggctc gtacaagctg accggccggc gcatcttccg gatggcgccg
    14761 atccatcacc acttcgaact caagggctgg ccggagccgc gcgtgatcgt gcgcttctgg
    14821 atcatcacgg tgatcctcgt gatgatcggc ctgtccacgc tgaagatccg gtgagccgcc
    14881 catggtcatg gtcccggctc cccgtcccga tctccacggc ctgacgctgg tcgtcgggct
    14941 cggcgtctcc ggcctgtcgg cggcgcgcgc gctcgcgcac ctcggcgcga gcttcgccgt
    15001 cgtcgacacg cgcagcgcac cgcccgggct cgccgagctc gatcgcatcg cccctgaggc
    15061 cgaggtctgc tgcggcgact tcggattgat tcaggccgaa gccctgttcg cgcgcgccgc
    15121 ccggctgatc gtcagccccg gcatcagcgt gcgcacgccg gcgatcgccg cctgcgcggc
    15181 gcgcggcgtg ccggtctggg gcgacatcga actgttcgcg cgcctgacgg acgtgccggt
    15241 gatcggcatc accggctcga acggcaagtc gaccgtgacc acgctgctcg gcgagatggc
    15301 caccgccgcc ggcgtgcgtc cggcggtcgg cggcaacctc ggcacggcgg cgctcgatct
    15361 gtggctcgaa cgcgaggccg ccggcgacgt gccggggctg tacgtgctgg agctctccag
    15421 cttccagctc gaaaccacgc actcgctcgc ctgcgtggcc gcgaccgtgc tgaacgtcag
    15481 cgccgatcac ctcgaccgct acgacggcat ggacgactac gccgcggcca aggcgcggct
    15541 gttcgagcgc gccgccgtgc aggtcgtcaa ccgcgacgat gcgcgcgtgc gcgcgatgat
    15601 ccggccggag cggccggtga tcggcttcgg cagcgaccgg ccgccggctg ccggcgacta
    15661 cggcctcgaa cgccacggcg acacgatccg gctgatgcac ggcgccacgg cgctgctcga
    15721 tgccgcggag cttcgcatcg gcggcctgca caacgccctg aacgtgctcg cggcgctcgc
    15781 actcggtgcg gccgccggcc tgccgctggc gccgatgctg gcggcggcgc gggccttcgc
    15841 cggccttgcg caccgcacgc agctggtcgc cgagcacgcc ggcgtgcgct ggtatgacga
    15901 ttccaagggc accaacgtcg gggcgaccgt cgcggcgatc gccggcctgc ccggccggct
    15961 ggtgctgatc gccggcggcg acggcaaggg ccaggacttc gcgccgctcg ccgaggccct
    16021 cgacggccgc gcccgcgccg tcgtgctgct cgggcgcgat gcgccgcgca ttgaggcagc
    16081 gcttgccggg cgcctgccct gcgagcgggt cgcggacatg gagcaggcgg tcgcgcgcgc
    16141 cgccgcgctc gcgcagcccg gcgacatcgt gctgctgtca ccggcctgcg cgagtctcga
    16201 catgtaccgc agctacgccg aacgcggccg gctgttcgcg caggctgcac ggcgggtggc
    16261 cggctcatgc tgaacgtcgt catcgaccgc gtgcgccacg tcgcgcccga ccatgaccgg
    16321 ctggcaccgc tcgacccgtg gctggtgttc ccggccatcg gcctgatcgc cttcggcgtg
    16381 ctgatggtga cctcggcctc gatgccgatc gccgaccgtc aggccgatgc gccgctgtac
    16441 ttcctgtacc gcgagttcgc ctacgtcgcc ctcggcctgc tgctcggctg gctggccttc
    16501 caggtcgagg tcgagacctg gtatcgccgc ggaccggcga tcctgatggt tgtcggcgtg
    16561 ctgctggtgc tggtgctgat ccccggcatc ggccggcgcg tcaacggttc gtggcgctgg
    16621 atcaacctcg gcatcatgaa cgtgcaggtc tcggagctcg ccaagctcgc gtcgatcctg
    16681 tacgtcgccg gctatctgcg ccgccacgcg caggcgctgc gcgagtcgat cgtcgcgatg
    16741 atccggccgc tgatggtgct cggcgtgctg gcgctgctgc tgctgatgga accggacttc
    16801 ggcgcgacca tggtcatcat cgccgtcgcg ctcgggctgg tgttcctcgg cggcgtcaac
    16861 ttctggcagt tctcggcgat gcagctgctc gtggtcggcg gcatggccgt gctgctgtgg
    16921 tcctcgccgt accggcgcgc gcgcctgtcg agcttcctga acccgtggga agacccgttc
    16981 aacaagggct tccagctcac gcagtcgctg atcgcgatcg gccgcggcga gttcgacggc
    17041 gtcggactcg gcgccagcgt gcagaagctc ttctacctgc ccgaggccta caccgacttc
    17101 atcttcgcga tcatcgccga ggagctcggc ctgctcggcg tgctctgcgt gctcgcgacc
    17161 tatctggtca tcgtcgtgcg cagcttccag gtcggcgcgc gctgcgagcg cctgcgccag
    17221 cccttcggcg cctcggtggc ctacggcatc ggcctgtgga tcggcttcca ggcgctgatc
    17281 aacatgggcg tgaacatggg cgtgctgccg accaagggcc tgacgctgcc gttcatcagc
    17341 tacggcggct cctcgctggt cgtcatgtgc atcgcctacg cgatcctgct gcgcgtcgat
    17401 gccgaatcgc ggctcgaaga ggacccgaag ctcaggcgcg accggctgct tgcgaaggtg
    17461 cgcgcgccat gagcacgccg gttccgatgc cggccggcgg cgcgccggtg ctggtgatgg
    17521 ccggcggcac cggcggccac gtgttcccgg cgctcgccgt cgccgccgag ctgcgcgcgc
    17581 gcggcgtgcc ggtggtctgg ctcggcgcgc gcagcgggct cgaagccagg ctcgtgcccg
    17641 cgcacggctt tgcggtcgaa tggctggaag tcgccggcct gcgcggcaag ggctgggcgc
    17701 ggcggctggc cgcgccgctg atgctcgcgc gtgcgctctg gcaggccgcg cgcgtgctgc
    17761 gccggctgcg gccgcggctg gtgctcggct tcggcggtta cgtcagcggt ccggccggac
    17821 tgatggcgcg cctgctcggc gtgccgctgc tgatccatga gcagaacgcc atcgccggct
    17881 acaccaaccg catcctcgcg cggctcgcga cccgcgtctg cgaggccttc cccggcgcct
    17941 tcccggccgc cctcggcgcg gtcacgaccg gcaacccggt gcgcgcggcg atcgcgcagc
    18001 tgccgtcgcc ggtcgtgcgc ggcgcgacgc gtggcggccg cccgcgcctg ctggtg
//
LOCUS       DAOQIE010000030        18042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1442_length_18042_cov_8.187024, whole genome shotgun sequence.
ACCESSION   DAOQIE010000030 DAOQIE010000000
VERSION     DAOQIE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 18042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18042
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_18042_cov_8.187024"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK144_03430"
     CDS             complement(<1..792)
                     /locus_tag="PK144_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF64807.1"
                     /translation="MPRPDLPSAVSRRPSPLLLLAFAVAALVALPLAGIVAQFTEPGT
                     AATWRHLAAHVLPEYLANTALLCLSTGLAVAVIGGGTAWLVAMHDFPGRRWLEWALVL
                     PLAMPAYVLAYVYTDLLQYAGPVQSGLRAAFGWQRGDYWFPEIYGLGGAATVFAASFY
                     VYVYLLARTAFLERAGGAAEAARSLGCGAWGAFLRVSLPLARPAIAAGTALALMETLA
                     DYGAVAYYALPTFTTGIYRAWFALGDRAAAAQLAATLLGFVLLLLT"
     gene            866..1915
                     /locus_tag="PK144_03435"
     CDS             866..1915
                     /locus_tag="PK144_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64808.1"
                     /translation="MALLSIEHVEQGYGARIAVADLSLTLAAGEIGCLLGPSGCGKTT
                     LLRGIAGFEPLAAGRIRLGGRTLAEPGRELPPEQRGVGMVFQDYALFPHLDVAANVGF
                     GLKGSKPLARTEREHRVATLLDLVGLKDAGRRYPHELSGGQQQRVALARALAPQPQLL
                     LLDEPFSSLDASLRERLALDVRTLLKALGTTAILVTHDQLEAFALADRIGVMHGGRLR
                     QWATPWQVYHRPADRLVAGFVGQGVWLRGERIDDRHVQLPDLGPVPVPDAPAARWLDV
                     LLRPDDFVRDTASPLRAEVVGRAFRGAECLYTLRLPGGAEVLALLPSQHDHAPGERIG
                     LRLDVDSVTGFAAGG"
     gene            2091..2564
                     /locus_tag="PK144_03440"
     CDS             2091..2564
                     /locus_tag="PK144_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64809.1"
                     /translation="MDNHHLAVSLPADRLTRVLNLLRKLEAELPFLIMLSDEERDAMP
                     ILDERKLAYIDRALHLVEAHPDFMPRDFEADEFGTDVQLVKALGLIHSHLLPLARKLD
                     DSLRCAENDACRAALEVHRHASLEDPADTDSLDAMIDSLSPRRFRRCHVSNPAHP"
     gene            2654..>5970
                     /gene="recC"
                     /locus_tag="PK144_03445"
     CDS             2654..>5970
                     /gene="recC"
                     /locus_tag="PK144_03445"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNF64810.1"
                     /translation="MLHLYHSNRMEILAAQLAARLGEPVDDGWAPETLVVASNGMRRW
                     LCLDLADRLGICANLHFPLPSRLFWDIYRAVLPGVPTESAFATPILAWRVLAEFGGLD
                     DDPVWEPLRDYLAGSDDYGRYELARRIADTFDQYLVYRPKWIKNWEKGGAAPGGKGRE
                     EDEDDEDGDDCEGGWQAALWRRLSEVDATHRARLHRQFLKALKQLHERPAGIPARLSF
                     IGIPALPAAWLQTLLGLARWCEVDLYLLNPCAQFWGMIESERTIAKIAPEADHAALYL
                     ETGNRLLASMGRLGRDFHHLLADSTSDLPAAEAFEEPEADTLLAVVQRDLLRLENRGA
                     NHAPSEHDGFTLWAEPYLAAADDHSIQMHVCHSATREIEVLHERLLALFAADDSLAPT
                     DVVVMTPDIEAYAPAVEAVFGAAESGLFIPYGIADRGVRAESPLLDAFLALLELPGGR
                     WGAETVASLLDAAALRRRFGILEEDLGTLRDWLTATGVRWGLDADHRQSLELPAESAH
                     TWRAGLDRLLLGIALPGDGERLYAGVLPYDEVEGHTAQLAGRLAQFVDRLAALESGLA
                     AARAPAGWSGTLAAVFEDFLAPEPDDEAEAARLRTAIQRLAETAAIAGFGGAVPLAVA
                     RRFLLAELEGMGSVQGFLAGGVTFCAMVPMRAIPFRVVCLIGMNDGAYPRVNRPHDFD
                     LIARHPRRGDRSRRNDDRYLFLEALVSARDVFYVSWTGRSQRDNAELPPSVLVAELLD
                     YIARAAQDPRDGAAEAGQPQADALEALRRVCVEHPLTPWGTAAFDGGDRRRASHSPRW
                     CRAAQLAGRGERMRREPCPEPLPDPETEWLTLSPERLTDFLCAPARFLLRERLRLRLD
                     EAEGELAETEPFLLDDFADRRLRGELLRLRQRGLDPDAARRVLAGSGLLPHGAVGRLL
                     YAREAAAVETVLARLPAGGDTVLLEPLPVDLTLAGVRLLGWLREVGGHGILVAEPNPP
                     GWRERLRLWVRHLLLCRAVQAGLRMPDGTEVPARSLCHGPDAGFAFTPVANPDAVLEP
                     LLALYRSGLLRPSAFIPPVAWAWWQGKPDDTPERAIAQALRKWEGDDFTGSHACADDR
                     WNRCAYDGVLPAADAWQT"
     assembly_gap    5971..6070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6207..6431
                     /locus_tag="PK144_03450"
     CDS             6207..6431
                     /locus_tag="PK144_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018878730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNF64811.1"
                     /translation="MNDTVTLDPQGRIDIPETIREALHLQSGQRFVIVTHDDSITLVP
                     ERSIAWARGILKGADPANHRDRESTADDPS"
     gene            6418..6813
                     /locus_tag="PK144_03455"
     CDS             6418..6813
                     /locus_tag="PK144_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019864468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF64812.1"
                     /translation="MTLHEPRVLVDTCGWIEWLTDGVLASHFEPYLADPAHLVVPSIL
                     QFELYRWIRRERGESLALQIIAITHQGQLMGIDARLAILAADLALQYRLAMADALIYA
                     CAQSAGCELISSDRHFCGLPGVVHFQKRA"
     gene            6843..10544
                     /gene="recB"
                     /locus_tag="PK144_03460"
     CDS             6843..10544
                     /gene="recB"
                     /locus_tag="PK144_03460"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HNF64813.1"
                     /translation="MSDMHPLDVLNVPLDCANLIEASAGTGKTYAIASLVLRLVAERG
                     VRIDRILVVTYTKAATAELRGRIRMRLVQARDAFRGAPTDDAVCRALIARAEQQEREN
                     RKRREHRAAHEKPEGKAQERADEGGTLIRWARRLDAAVLAFDEAAIFTIHGYCQRALA
                     DHAFAAGESFETELLADDSGLLEEVVQDFWRLQLYNAAPVLVMQALDAGLTPARLAQQ
                     IRGCLGKPYLSYDEPPAPDIDTAQAAFERAFAVAAECWQRQGIEVGTLLHQAELSRTS
                     YRLASIPGWCAELDAWFAGEPKAALPEKFAQFTSAKLVKATKKGATPPRHAFFDCADG
                     LAAAVEALATAFEGWLARLRWQLLRWCSEELPRRKQRLQKLAYDDLLTRLAAALASGD
                     LGKRLSESLAGRYEAALIDEFQDTDPTQYEIFRAIYTGRSQPVFFVGDPKQAIYAFRG
                     ADVFAYLRASDDAPARHTLAVNWRSSPALIRAINALFGLRPDAFLLRRIHFHPVEASP
                     AARPDFVAPDGRAALQLWRLPPGEGDKALGKGQANALVAAATAQRIAELLKAGQKGEA
                     RIGARALHGGDIAVLVRSHTQSAAIRDALLALGVPCVLQSRDSVFASREAELLMRLLA
                     AVLDPRHEGRVRALLLTDACGLDLVQLWALGQDTKPWEAMLERFHRLRTIWFGRGGFP
                     AMFRHWLSDFDVAARLLEWRDGERRLTNLLHLAELLAAAIAEDRLTPEASLAWLSDAR
                     AAAGVEDETRQLRLDSDAQRVQIVTIHRSKGLEYPVVFCPYLWNGRLWANDEVLVRAH
                     EGNPENPDDSASGRSRLEFAAGPDSELRRQAVREELAENLRLAYVALTRARYRCYLVW
                     GAASESERSALAWLLHPHRLPDAAAVLAAGNGRKSWLADDLLRADLDALLAAAGDTCT
                     IQPLPQTGPGLRYRPALPPAERLQAAGFGRRLPTGRRTTSFTQLAEGRSHEGPDRDTL
                     AAQPPAKPAAAQSPVETAAPRRDFRSFPRGAEAGTCLHAMLERLDFAADDEAIGLIAA
                     EELKRAGIAADWLPAAVDGIRATLTTALDDSGLRLCELPADARRAELEFTFPLGRIRA
                     DGLGAVLRAHGVSMPGLAFKDIEGYLRGFIDLVCEADGRVFIVDYKSNWLGPEAGDYH
                     VERLPAAMDAAGYRLQYLLYCVAIVRFLRRRRPGWRYADGFGGVRYLFLRGLDPALGS
                     THGVFAERPAEALIDALDAYLASGDVMSA"
     gene            complement(10600..11082)
                     /locus_tag="PK144_03465"
     CDS             complement(10600..11082)
                     /locus_tag="PK144_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF64814.1"
                     /translation="MAGNRRPDTGRWDTALDPLCVPLFLSLQWARSRSLAHMQPVLSR
                     HRLSAAEFDVLATLRNSAPPHRLTPSQVRDQVVITSGGLTKLMLQLEARGLLLRPPSA
                     GDQRVKPVQLSEAGRQAIEAAMADMLVGLRAWLTQALGEDEITCLTGLLQRLADAGPP
                     "
     gene            11229..12563
                     /locus_tag="PK144_03470"
     CDS             11229..12563
                     /locus_tag="PK144_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNF64815.1"
                     /translation="MRVIQRVFLAFPVLLTALWILGDPGAFSGLNGFFAWRNVLMQYS
                     GVLGIGVMSFAMLLSLRPAWFERRLGGLDKLYRLHRWLGIAGLSITVAHWLLAQGPKW
                     LVQLGWLQRGNRPPRPLLPEGSLQQWFAHQRGFAEGVGEWAFYAAVVLMLLALVKWFP
                     YRRFFQTHRLLAVCYLALVFHSVVLLRFEYWSTPLGAGLALLMAGGCVGAVMALLRRH
                     LGGRRIRGRVVGLQRQPEIGVLGVDIEPDAGWPGHEAGQFAFISFDDAEGPHPFTIAS
                     AWSGEPRLRFLIKALGDYTRLLPGRLHVGSVVTLEGPYGRFTFEGDARRQIWIGGGIG
                     ITPFLARLQALGSAPDGRDIDLFHSTAQYDPDVIARLQREATAAKVRLHVLWDPRDGR
                     LDVAGIVSQVPDWREADVWFCGPGGFGRAIRDGLLRLGLPAERFHQELFEMR"
     gene            complement(12630..13961)
                     /locus_tag="PK144_03475"
     CDS             complement(12630..13961)
                     /locus_tag="PK144_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HNF64816.1"
                     /translation="MWTFWLEFILVLGAILLGIRRGGVALGLIGGLGVAVLVFVFRTP
                     PSEPPVTVMLIILAVVTASATLQVAGGLDWLVQQTERLLRAYPQRVTLLAPLATFLLT
                     VCVGTGHAVYALLPVIGDVALRTGIRPERPMAISSVASQMGITASPVAAAVTTFLAFA
                     AKTPTPVGLFDIVMITLPAGLAGVLVAALWSMKRGKDLADDPEYQARLADPQFRAALD
                     VSVTTLGTELPKTAKLSVALFFGGVLTIVMLALFPVLVPVFGGKAVPMTTIVQIVMLG
                     FGALILFATGVKAPDIARSSVFTAGMIAVVSIFGIAWMSDTFVSGNKAFLIEQIEAMV
                     KFAPWTFALAMFAVSAFVKSQAATLTIMIPFGLALSLPVPLLLGLMPSSYAYFFFAFY
                     PSDLAAINMDRTGTTHIGKYLLNHSFMIPGLIGVGVSTTVAYLLSRVLFSY"
     gene            14346..14810
                     /locus_tag="PK144_03480"
     CDS             14346..14810
                     /locus_tag="PK144_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HNF64817.1"
                     /translation="MQSRRNASLFVRFASATARCSGRPVTFVLAFLVIVVWGVTGPLF
                     HYSDTWQLVINTGTTIVTFLMVFLIQNTQNRDAEAVQVKLDELIRATRGAHNALLNLE
                     ELEDRDIERIRVRYQRLAEEARAALRSDGGGDTDTPEAGEDGAAADGGRRQD"
     gene            14929..15372
                     /locus_tag="PK144_03485"
     CDS             14929..15372
                     /locus_tag="PK144_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64818.1"
                     /translation="MNILLRSHADRRLPALTLAAALGLTLAGCAGAPVATTGSAPLTD
                     AGGRTLYTFDKDGDGVSRCAATCTATWPPYVVKAGENPAGMSAITREDGSRQWSLAGR
                     PLYFFAGDQKPGDARGDGVGGVWHLARNAAPAASAASSSGSSYGY"
     gene            15516..16451
                     /locus_tag="PK144_03490"
     CDS             15516..16451
                     /locus_tag="PK144_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF64819.1"
                     /translation="MSAIAPSAASCTSSSDRFSGRSASRRPARWRRQLLGAALAATAA
                     ASAGASPLLFSGTGAAATTAFDAFRGAIGGGSRITWDGVRLDGSDVNPATQVLIPGST
                     VAIPENRFTAAGALFADPYAVAGDGFAAANPATAGQFPAFTPGNTFVMFDTDGSDFDD
                     RFIEQSFVLPGTTTAAGTRGFGAIFVGVEQAGSSSIEYFGRNALGERISLGHFAVEAG
                     SGPSDTQFLGVLFDAAIVADVVLTVGTHALFGFDGTTLRSFGAQTPGTDLAVTDDFVF
                     ARPEALVTVPEPPPLALGFAALGAALTLRRLRPAD"
     gene            16609..17919
                     /locus_tag="PK144_03495"
     CDS             16609..17919
                     /locus_tag="PK144_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF64820.1"
                     /translation="MQDLHGHALSGASAQALDAFEIALRELRCLSGDPLGHAEAAVAA
                     APEFTMAHALRAWLFLVGTDAGAVPLAREALAQARALPADAREARHLAAIGHWCAGRW
                     RLAARTLEDLSLEHPHDLLALQLGQQLDFFTGDQRLLRDRIARALPAWSPDMPGYHAL
                     LGMYAFGLEENGDFHNAERYGRRAIELEPRDAWAQHAVVHVLEMQGRRDDGIRWMTGQ
                     SGWKQCGLLAVHNWWHLALHYLGLDDVDAALQLLDGPIDGHRPTLALELIDVSALLWR
                     LRLRGVDVGERWVSVAERWSACAGHGWYAFNDLHAAMAFACAGRADLLEQLDATQRAA
                     QTRADDNAGFLLAVGRAATEAVIAHVAGDHRRTVERLRDLRSHARAFGGSDAQRDLLD
                     QTLIHSARASGQPALARGLEAERSLLARQRAGARPAVALQPAMH"
BASE COUNT         2596 a         6368 c         6300 g         2678 t
ORIGIN      
        1 cgtcagcagc agcagcacga agccgagcag cgtcgccgcg agctgcgcgg cggcggcgcg
       61 gtcgccgagc gcgaaccacg cgcgatagat gccggtcgtg aaggtcggca gcgcgtagta
      121 cgcgaccgcg ccgtagtcgg cgagggtttc catcagcgcc agcgccgtgc cggcggcgat
      181 cgccgggcgc gccagcggca gcgacacgcg caggaacgcg ccccaggcgc cgcagccgag
      241 gctgcgtgcg gcttcggccg caccgccggc gcgctccagg aaggccgtgc gcgccagcag
      301 gtagacgtag acgtagaagc tcgcggcgaa cacggtcgcg gcgccgccga ggccgtagat
      361 ctccgggaac cagtagtcgc cgcgctgcca gccgaaggcc gcgcgcagcc ccgactgcac
      421 cgggccggcg tactgcagca ggtccgtata cacgtaggcg agcacgtagg ccggcatcgc
      481 cagcggcagc accagcgccc attccagcca gcgccggccg gggaagtcgt gcatcgccac
      541 cagccaggcc gtgccgccgc cgatcaccgc gaccgcgagg ccggtgctga ggcacagcag
      601 cgcggtgttg gcgaggtatt cgggcagcac gtgcgcagcc agatgccgcc aggtggcggc
      661 ggtgccgggt tcggtgaact gcgcgacgat gccggcgagc ggcagcgcca ccagcgccgc
      721 gaccgcgaag gcgagcagca gcagcggcga aggacggcgg gaaacggcgg acgggagatc
      781 gggacggggc atcggcgcag gtggcgtggc gttttatgat cgacaacaga gaacgattct
      841 cacatgggag acccccggag acacgatggc gctgctttcg atcgaacacg ttgaacaagg
      901 ctacggcgcc cggatcgcgg tcgcggacct gagcctgacg ctggcggccg gcgagatcgg
      961 ctgcctgctc ggtccgtcgg gctgcggcaa gaccacgctg ctgcgcggca tcgccggctt
     1021 cgagccgctc gccgccggcc gcatccgcct cggcggacgc acgctcgccg aacccgggcg
     1081 cgagctgccg cccgagcagc gcggcgtcgg catggtgttc caggattacg cactgttccc
     1141 gcatctggac gtcgccgcca acgtcggctt cggcctgaag ggttcgaagc cgctggcgcg
     1201 caccgaacgc gagcaccggg tcgcgacgct gctcgatctg gtcggcctca aggacgccgg
     1261 ccggcgctat ccgcacgagc tttccggcgg tcagcagcag cgcgtggcac tggcgcgcgc
     1321 gctggcaccg cagccgcagc tgctgctgct cgacgagccc ttctccagcc tcgacgccag
     1381 cctgcgcgag cggctggcgc tcgatgtgcg cacgctgctc aaggcactcg gcacgacggc
     1441 gatcctggtc acgcacgacc agctcgaagc cttcgcgctc gccgaccgca tcggcgtcat
     1501 gcacggcggg cggctgcgcc agtgggcgac gccgtggcag gtctatcacc ggccggccga
     1561 tcgcctcgtc gccggcttcg tcggtcaggg cgtgtggctg cgcggcgagc gcatcgatga
     1621 ccgccacgtg cagctgcccg acctcggtcc ggtgccggtg ccggatgcgc cggcggcaag
     1681 atggctggac gtgctgctgc gccccgatga cttcgtgcgc gacacggcct cgccgctgcg
     1741 cgccgaggtc gtcgggcggg cgttccgcgg tgccgagtgc ctgtacacct tgcggctgcc
     1801 cggtggcgcc gaggtgctgg cgctgctgcc ttcgcagcac gatcacgctc cgggcgagcg
     1861 catcggcctg cgcctcgatg tcgactccgt gaccggattt gccgccggcg gctagcgatc
     1921 cggcacatct gcgactattt cgtcatgtct gatgcggtgg ctacggcatc tgtcgaagtg
     1981 actgccctct gacctctgcc aatactccgt ccgcgcgctg ccgtgccatg ccgtgctgtt
     2041 accgtccccg acctgctgcc catgtccccg gtactgaagg aaggaacgag atggacaacc
     2101 accaccttgc cgtgagcctg cctgccgatc gcctgacccg tgtgctgaac ctgctgcgca
     2161 agctcgaagc cgaactgccg ttcctgatca tgctgtccga cgaggagcgc gacgcgatgc
     2221 cgatcctcga cgagcgcaag ctggcctaca tcgaccgggc gctgcacctc gtggaggcgc
     2281 atccggactt catgccgcgc gacttcgagg ccgacgaatt cggcaccgac gtgcagctgg
     2341 tcaaggcgct cggcctgatt cacagccatc tgctgccgct ggcgcgcaag ctcgatgatt
     2401 cgctgcgctg cgccgagaac gacgcctgcc gcgccgccct cgaagtccat cgccacgcca
     2461 gcctcgagga cccggccgat accgacagcc tcgatgcgat gatcgacagc ctgagtccgc
     2521 gccgcttccg ccgctgccac gtgagcaacc cggcgcatcc ctgaacggct gacctgaacg
     2581 gctgacggag ggccggcgcc tgcgtgccat actccggcgc ccgccggcct cccgtcgtct
     2641 ccgacccccg cgcatgctgc acctgtacca ctccaaccgc atggaaatcc tggccgcgca
     2701 gcttgccgcg cggctcggcg agccggtcga tgacggctgg gcaccggaga cgctggtggt
     2761 cgcctcgaac ggcatgcgcc gctggctgtg tctcgatctc gccgaccggc tcggcatctg
     2821 cgccaacctg cacttcccgc tgccgtcgcg gctgttctgg gacatctatc gcgccgtgct
     2881 gcccggcgtg cccaccgaat cggcgttcgc gaccccgatc ctcgcctggc gcgtgctcgc
     2941 cgagttcggc ggtctcgatg acgatccggt ctgggagccg ctgcgggact atctcgccgg
     3001 cagcgacgac tacggccgct acgagctcgc gcggcgcatc gccgacacct tcgaccagta
     3061 cctggtctat cggcccaaat ggatcaagaa ctgggagaaa ggtggcgcgg ccccgggcgg
     3121 caagggcagg gaggaagatg aggacgatga ggacggtgat gactgtgagg gcggctggca
     3181 ggcggccttg tggcggcgct tgtcggaggt cgatgccacg caccgcgcac gcctgcaccg
     3241 gcagttcctc aaagccctga agcaattgca cgagcgtccg gccggcatcc cggcgcggct
     3301 gtccttcatc ggcatcccgg cgctgccggc ggcctggctg cagacgctgc tcgggctggc
     3361 gcgctggtgc gaggtcgacc tgtacctgct caatccctgc gcgcagttct ggggcatgat
     3421 cgagtccgag cgcacgatcg cgaagatcgc acccgaggcc gatcacgcgg cgctgtacct
     3481 cgaaaccggc aaccgcctgc tcgcatcgat gggccggctc ggccgcgatt tccatcatct
     3541 gctcgccgat tccaccagtg acctgccggc cgcagaggct ttcgaggagc ccgaggccga
     3601 tacgctgctc gccgtcgtgc agcgtgacct gctgcggctc gaaaaccgcg gtgccaacca
     3661 cgcgccctcg gagcacgacg ggttcacgct gtgggccgag ccctacctcg cggcagccga
     3721 cgaccactcg atccagatgc acgtctgcca ctcggcgacg cgcgagatcg aggtgctgca
     3781 cgagcggctg ctggcgctgt tcgcggccga tgattcgctc gcgccgaccg atgtcgtggt
     3841 catgaccccg gacatcgagg cctatgcacc ggcggtcgag gcggtgttcg gtgccgccga
     3901 gtccggcctg ttcatcccct acggcatcgc cgatcgcggc gtgcgcgccg aaagtccgct
     3961 gctcgatgcc tttctggcgc tgctcgaact gccgggcgga cgctggggcg ccgagacggt
     4021 cgcgagcctg ctcgatgccg cggcgctgcg ccggcgcttc ggcatccttg aggaggacct
     4081 cggcacgctg cgcgactggc tgacggcgac cggcgtgcgc tggggactcg atgcggatca
     4141 tcggcagtcg ctcgaactgc cggccgagtc cgcccacacc tggcgcgccg ggctcgaccg
     4201 gctgctgctc ggcatcgcgc tgcccggcga cggcgagcgc ctgtacgccg gcgtgctgcc
     4261 gtatgacgag gtcgaaggtc acaccgcgca gctcgccggg cggctcgcgc agttcgtcga
     4321 ccggctcgct gcgctcgaat ccgggctggc cgctgcccgc gcgccggccg gctggtccgg
     4381 cacgctggcg gcggtgttcg aggacttcct cgcgcccgag ccggacgacg aggccgaggc
     4441 cgcgcgcctg cgcaccgcga tccagcgtct ggccgaaacc gcagcgatcg ccggcttcgg
     4501 cggcgcggtg ccgctcgccg tcgcccggcg cttcctgctc gccgaactcg aaggcatggg
     4561 cagcgtgcag ggtttcctcg ccggtggcgt gaccttctgc gcgatggtgc cgatgcgcgc
     4621 gatcccgttt cgcgtcgtct gcctgatcgg catgaacgac ggcgcctatc cgcgcgtcaa
     4681 ccgcccgcac gacttcgatc tgatcgcccg ccatccgcgc cgcggcgacc gctcgcggcg
     4741 caacgacgac cgttatctgt tcctcgaagc gctggtttcg gcgcgcgacg tgttctacgt
     4801 gtcctggacc ggccgcagcc agcgcgacaa cgccgagctg ccgccgtcgg tgctggtcgc
     4861 cgaactgctc gattacatcg cccgtgccgc acaggacccg cgggacggcg cagccgaagc
     4921 cgggcagccg caggccgatg cgctcgaagc cctgcgccgg gtgtgcgtcg agcacccgct
     4981 gacgccctgg ggcacggcgg ccttcgatgg cggggaccgg cgccgcgcca gtcattcgcc
     5041 gcgctggtgc cgggccgcgc agctcgccgg gcgcggcgaa cgcatgcgcc gtgagccgtg
     5101 cccggagccg ctgccggacc cggaaaccga atggctgaca ttgagcccgg agcggctgac
     5161 cgatttcctg tgcgccccgg cgcgcttcct gctgcgcgag cgcctgcgcc tgcgcctcga
     5221 cgaagccgaa ggcgaactgg ccgagaccga acccttcctg ctcgatgact ttgccgaccg
     5281 gcggctgcgc ggcgaactgc tgcggttgcg ccagcgcggt ctcgacccgg atgcggcccg
     5341 gcgcgtgctc gccggttccg gcctgctgcc gcacggcgcg gtcgggcgcc tgctgtatgc
     5401 gcgcgaagcg gcggccgtcg aaaccgtgct cgcccgcctg ccggccggcg gcgacacggt
     5461 gctgctggaa ccgctgccgg tcgatctgac gctcgccggc gtgcggctgc tcggctggct
     5521 gcgtgaagtc ggcggacacg ggattctcgt cgccgagccg aatccgccgg gctggcgcga
     5581 gcgcctgcgg ctgtgggtgc ggcatctgct gctctgccgg gcggtgcagg cgggcctgag
     5641 gatgccggac gggaccgaag tccccgcccg cagcctctgc cacggtccgg acgccggctt
     5701 tgccttcacg ccggtcgcga atcccgacgc cgtgctggag ccgctgctgg cgctgtatcg
     5761 cagcgggctg ctgcgcccgt cggcattcat cccgccggtg gcctgggcct ggtggcaggg
     5821 caagcccgac gacacgccgg agcgggccat cgcgcaggcc ctgaggaaat gggaaggcga
     5881 cgatttcacc ggcagccacg cctgcgccga cgatcgctgg aaccgctgtg cctacgacgg
     5941 cgtgctgccg gcggccgatg cctggcagac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn gctggaaccg ctgtgcctac gacggcgtgc tgccggcggc cgatgcctgg
     6121 cagacggtgg cgcgcggcgt gttcgcggtg atgacggggc atgagactac tatcggggac
     6181 tctgccccag atacgggaat ccgctcatga acgataccgt cacgctcgat ccgcagggcc
     6241 ggatcgacat tccggaaacc attcgcgaag ccctgcacct gcagagcgga cagcggttcg
     6301 tcatcgtcac gcacgatgac tcgatcacgc tggtgccgga gcgttcgatc gcctgggcac
     6361 ggggcatcct gaaaggagct gatccggcga atcaccgtga tcgggagtcc acggccgatg
     6421 acccttcatg aaccccgggt cctggtcgat acctgcggct ggatcgaatg gctgaccgac
     6481 ggcgtgctgg ccagccactt cgagccgtat ctggcggatc cggctcatct cgtcgtgccg
     6541 tcgatcctgc agttcgagct gtaccgatgg atccggcgcg aacgcggcga atcactggcc
     6601 ctgcagatca tcgccatcac ccatcaggga cagctcatgg gcattgatgc gcgtctggcc
     6661 atcctggcag ccgatctggc cctgcagtac cggctggcga tggcggatgc gctgatctac
     6721 gcatgtgcgc agtccgcagg ctgcgaactg atcagctcgg accggcattt ctgcggcctg
     6781 cccggcgtcg ttcatttcca gaaacgtgca tgagtccgaa cgctgagccc ggaacaccgc
     6841 gcatgtccga catgcacccg ctcgacgtcc tgaacgtccc gctcgattgc gccaacctga
     6901 tcgaggccag cgccggcacc ggcaagacct atgcgatcgc gagcctggtg ctgcggctgg
     6961 tggccgagcg cggcgtgcgc atcgaccgga ttctggtcgt cacctacacc aaggccgcga
     7021 ccgccgaact gcgcggacgc atccgcatgc ggctggtgca ggctcgcgac gcgtttcggg
     7081 gcgccccgac cgacgatgcc gtctgcaggg cgctgatcgc acgggcagaa cagcaggagc
     7141 gggaaaacag gaagcgccgt gagcacaggg cagcccacga aaaaccggaa gggaaagctc
     7201 aggagcgcgc ggacgaaggc ggaacgctga tccgctgggc ccggcggctc gatgccgcgg
     7261 tgctcgcctt cgacgaggcg gcgatcttca cgatccacgg ctactgccag cgcgcgctgg
     7321 ctgatcacgc cttcgccgcc ggcgagtcct tcgagaccga actgctcgcc gacgactccg
     7381 gcctgctcga agaggtcgtg caggatttct ggcgtctgca gctctacaac gcagcgccgg
     7441 tgctggtcat gcaggcgctc gatgccgggc tgacgccggc acgcctcgcg cagcagatcc
     7501 gcggctgtct cggcaagccc tacctgagct atgacgaacc gccggccccg gacatcgaca
     7561 cggcgcaggc tgcgttcgag cgtgccttcg cggtggcggc cgagtgctgg cagcgtcagg
     7621 gcatcgaggt cggcacgctg ctgcatcagg ccgagctgag ccgcaccagc taccggctgg
     7681 cttcgattcc cggctggtgc gccgaactcg atgcctggtt cgccggggag ccgaaagccg
     7741 cgctgccgga gaagttcgcg cagttcacgt ccgcgaagct cgtgaaggcg acgaagaagg
     7801 gcgcaacgcc gccgcggcat gcgtttttcg actgcgccga cgggctggct gcagcggtcg
     7861 aggccctcgc caccgccttc gagggctggc tcgcacggct gcgctggcag ctgctgcgct
     7921 ggtgcagcga ggaactgccg cggcgcaagc agcggctgca gaagctcgcc tatgacgacc
     7981 tgctgacgcg gctggccgcg gcgctcgcga gcggcgacct cggaaagcgg ctgtccgaaa
     8041 gcctcgccgg acgctacgag gcggcactga tcgacgaatt ccaggacacc gacccgacgc
     8101 agtacgagat cttccgcgcc atctacacgg gccgcagcca gccggtgttc ttcgtcggcg
     8161 atccgaaaca ggcgatctac gccttccgcg gcgccgacgt gttcgcctac ctgcgcgcca
     8221 gtgacgatgc accggcccgg cacacgctgg cggtcaactg gcgctcctcc ccggccttga
     8281 tccgcgccat caacgcgctg ttcggactgc ggcccgatgc cttcctgctg cgccgcatcc
     8341 actttcatcc ggtcgaagcc tcaccggcgg ccagaccgga cttcgtcgcg ccggacggcc
     8401 gggccgcgct gcagctctgg cggctgccgc ccggtgaggg cgacaaggcc ctcggcaagg
     8461 gacaggccaa tgcgctggtg gccgcggcga ccgcgcagcg catcgccgaa ctgctgaagg
     8521 ccggccagaa gggggaggcg cgcatcggcg cgcgggccct gcacggcggc gacatcgccg
     8581 tgctggtgcg ctcgcacacg cagtcggcgg cgatccgcga tgcgctgctc gccctcggcg
     8641 tgccctgcgt gctgcagtcg cgcgacagcg tgttcgcaag ccgcgaggcc gagctgctga
     8701 tgcgcctgct ggcggccgtg ctcgatccgc ggcacgaagg acgcgtgcgc gcgctgctgc
     8761 tgaccgacgc ctgcggcctc gacctggtgc agctctgggc cctcggtcag gacacgaagc
     8821 cctgggaggc gatgctcgaa cgcttccacc ggctgcgcac gatctggttc ggccgcggcg
     8881 gctttccggc gatgttccgc cactggctca gcgatttcga tgtcgccgcc cgcctgctcg
     8941 aatggcgcga cggcgagcgg cggctgacca atctgctgca tctggccgag ctgctggccg
     9001 ccgcgatcgc cgaggaccgc ctgacgccgg aggcgagcct cgcctggctc agtgatgcgc
     9061 gggcagcggc cggggtcgag gacgagaccc gtcagctgcg cctcgacagc gatgcgcagc
     9121 gcgtgcagat cgtcacgatc caccgcagca agggcctcga atacccggtc gtgttctgcc
     9181 cgtatctgtg gaacggccgg ctgtgggcga acgacgaggt gctggtgcgc gcccatgagg
     9241 ggaatccgga gaatcccgac gattccgcct ccggccgcag ccggctcgaa ttcgccgccg
     9301 gtcccgacag cgagctgcgc aggcaggccg tgcgcgagga actcgccgag aacctgcgcc
     9361 tcgcctacgt ggcgctgacg cgggcgcggt atcgctgcta cctggtctgg ggcgcggcca
     9421 gcgaatccga acgcagcgcg ctcgcctggc tgctgcaccc gcaccgtctg cccgatgccg
     9481 ccgccgtgct cgctgccggc aacgggcgca agtcctggct cgccgatgat ctgctcaggg
     9541 ccgatctcga cgcgctgctc gccgccgcag gcgacacctg cacgatccag cccctgccgc
     9601 agaccgggcc gggcctgcgc taccgtcccg ctctgccgcc ggccgagcgc ctgcaggccg
     9661 cggggttcgg gcgccggctg ccgacggggc ggcgcacgac cagcttcacg cagctcgccg
     9721 aaggccgcag ccacgaaggc ccggaccgcg acacgctcgc cgcgcagccg ccggcgaaac
     9781 cggcagcagc gcagtcgccg gtcgaaaccg ccgcgccccg gcgcgatttc cgcagcttcc
     9841 cgcgcggcgc cgaggccggc acctgcctgc acgcgatgct ggaacgcctc gatttcgccg
     9901 ccgatgacga agccatcggg ctgattgcag ccgaggaact caaacgcgcc ggcattgctg
     9961 cggactggct gccggcggca gtcgatggca tccgcgcgac gctgacgacc gcgctcgatg
    10021 acagcggcct gcgcctctgc gagctgccgg cggatgcgcg ccgggccgag ctggagttca
    10081 cgtttccgct cgggcgcatc cgtgccgacg gcctcggggc cgtgctgcgg gcgcacggcg
    10141 tgtcgatgcc gggcctggcg ttcaaggaca tcgagggcta tctgcgcggc ttcatcgatc
    10201 tggtctgcga agccgacggg cgggtcttca tcgtcgatta caagtccaac tggctcggtc
    10261 ccgaggccgg cgactaccac gtcgaacgcc tgccggcggc gatggacgcc gccggctacc
    10321 ggctgcagta cctgctgtac tgcgtcgcga tcgtgcgctt cctgcgccgg cgccggcccg
    10381 gctggcgcta tgcggacggt ttcggcggcg tgcgctacct gttcctgcgc ggcctcgacc
    10441 cggcgctcgg ttcgacgcac ggcgtgttcg ccgagcgccc ggccgaggcg ctgatcgacg
    10501 cgctcgatgc gtatctggcc agcggcgatg tgatgagcgc ctgagcctcg gacgccggaa
    10561 tatgcggggc gcgcggacag ccggattgcg ggctgcccgt cagggaggtc cggcatccgc
    10621 cagccgctgc agcaggccgg tcaggcaggt gatctcgtcc tcgcccagcg cctgcgtcag
    10681 ccacgcccgc aggccgacca gcatgtcggc catcgctgct tcgatcgcct gccggccggc
    10741 ctcgctcagc tgcaccggct tcacgcgctg atcgccggcc gaaggcgggc gcagcagcag
    10801 gccgcgggct tcgagctgca gcatcagctt ggtcagcccg cccgaagtga tgacgacctg
    10861 atcgcgcacc tgcgacggcg tcagccggtg gggcggggcg ctgttgcgca gcgtcgccag
    10921 cacgtcgaac tcggccgccg acagccggtg ccggctcagc accggctgca tgtgcgccag
    10981 cgaacggctg cgtgcccatt gcagcgacag gaacagcggc acgcacagcg ggtcgagtgc
    11041 ggtatcccag cgcccggtgt ccggacgacg gtttccggcc atgcctgcag ttcctccggt
    11101 ggtggtgggg attgcaacgc aatcgcgggt cttgcgtaac gcagggtaac ttatcttgct
    11161 ggaaagatat tggctgccta gcattccggt gcatccctca tgcagaccag cgttcaggcg
    11221 gcgccatcat gcgtgtcatc cagcgggttt tcctggcatt tccggtattg ctcacggcgc
    11281 tctggattct gggcgatccc ggcgccttca gcggactgaa cggattcttt gcctggcgca
    11341 atgtcctgat gcagtacagc ggcgtgctcg gcatcggcgt catgagtttc gcgatgctgc
    11401 tgagtctgcg tccggcctgg ttcgagcgcc ggctcggcgg actcgacaag ctgtaccggc
    11461 tgcaccgatg gctcggcatc gccggactgt cgatcacggt tgcgcactgg ctgctggcgc
    11521 agggaccgaa atggctggtg cagctcggct ggctgcagcg cggcaaccgg ccgccccggc
    11581 ccctgctgcc cgaaggctcg ctgcagcaat ggttcgcgca tcagcgcgga ttcgccgaag
    11641 gcgtcggcga atgggcgttc tatgccgccg tggtcctgat gctgctggcg ctcgtcaaat
    11701 ggtttccgta ccggcgtttc ttccagacgc accggctgct tgccgtgtgc tatctggcgc
    11761 tggtgttcca ttccgtcgtg ctgctgcgct tcgaatactg gagcacgccg ctcggagccg
    11821 gacttgcact gctgatggca ggcggctgcg tcggggccgt gatggccttg ctgcgccgtc
    11881 acctcggcgg ccggcgcatc cgcggccgcg tggtcggcct gcagcgccag ccggagatcg
    11941 gcgtgctggg ggtcgacatc gagccggacg ccggatggcc gggccacgag gccggacagt
    12001 tcgcgttcat cagcttcgat gacgccgagg ggccgcaccc gttcacgatc gcgtcggcat
    12061 ggagcggcga gccgcgcctg cgcttcctga tcaaggcgct gggcgactac acgcgcctgc
    12121 tgccgggccg gctgcacgtc ggcagcgtcg tgacgctcga aggcccctac ggacgcttca
    12181 cgttcgaagg cgacgcccgc cggcagatct ggatcggcgg cggcatcggc atcacgccgt
    12241 tcctcgcccg cctgcaggca ctcggcagcg cgccggacgg ccgcgacatc gatctcttcc
    12301 acagcaccgc gcaatacgat ccggacgtga tcgcccggct gcagcgcgag gcgacggccg
    12361 cgaaggtgcg cctgcacgtg ctctgggacc cgcgcgacgg ccggctcgat gtcgccggca
    12421 tcgtcagcca ggtgccggac tggcgcgagg ccgacgtctg gttctgcggg ccgggcggat
    12481 tcgggcgcgc gatccgcgat ggcctgctgc gactgggact gccggccgag cgctttcatc
    12541 aggaattgtt tgaaatgcgc tgacgctccg gcaggacggg acgcagatgc cctgcgtccc
    12601 gcctccggct tgattgccgg tgtgacggct caataggaga acagcacccg cgacagcaga
    12661 tacgcgaccg tcgtcgaaac gccgacgccg atcagcccgg gaatcatgaa gctgtgattg
    12721 agcaggtact tgccgatgtg cgtggtgccg gtgcggtcca tgttgatcgc ggccagatcg
    12781 ctcggataga acgcgaagaa gaaatacgca taactcgacg gcatcagccc gagcagcagc
    12841 ggcacgggca gcgacagggc cagtccgaac ggaatcatga tcgtcagcgt cgcggcctgg
    12901 cttttcacga aagccgacac cgcgaacatc gccagcgcaa acgtccacgg cgcgaacttg
    12961 accatcgctt cgatctgctc gatcaggaag gccttgttgc cgctgacgaa ggtgtcgctc
    13021 atccacgcga tgccgaagat cgacacgacc gcgatcatgc cggccgtgaa cacgctggag
    13081 cgggcgatgt ccggcgcctt gacgccggtc gcgaacagga tcagcgcgcc gaagccgagc
    13141 atgacgatct gcacgatggt cgtcatcggc acggccttgc cgccgaacac cggcaccagc
    13201 accggaaaga gcgccagcat gacgatcgtg agcacgccgc cgaagaacag cgccaccgac
    13261 agcttcgcgg tcttcggcag ctcggtgccg agcgtcgtca ccgacacgtc gagtgcggcg
    13321 cgaaactgcg ggtcggcgag ccgggcctga tattcgggat catcggcgag gtccttgccg
    13381 cgcttcatgc tccagagggc cgccaccagc accccggcga gcccggccgg cagcgtgatc
    13441 atcacgatgt cgaacagccc gaccggcgtc ggcgtcttcg ctgcgaaggc gaggaaggtg
    13501 gtcacggccg cggcgaccgg gctggcggtg atgcccatct gcgaggcgac gctggagatc
    13561 gccatcggcc gctccgggcg gatgccggtg cgcagggcca cgtcgccgat caccggcagc
    13621 agcgcataga cggcgtggcc ggtgccgacg cacaccgtca gcaggaaggt cgcgagcggc
    13681 gcgagcagcg tcacgcgctg cgggtaggcg cgcagcagcc tttcggtctg ctgcaccagc
    13741 cagtcgagac cgccggcgac ctgcagcgtc gccgaggccg tgaccacggc gaggatgatc
    13801 agcatcacgg tgaccggcgg ttccgacggc ggcgtgcgga acacgaacac cagcacggcg
    13861 acaccgaggc cgccgatcag cccgagcgcc acgccgccgc ggcggatgcc gagcaggatg
    13921 gcgccgagca ccaggatgaa ttcaagccag aaggtccaca tcgcacttct cccgggggga
    13981 cgggcaagac cacgcaccgg ctcgcgccgg acgactgcca ccggtcccgg cgcgggaggg
    14041 cggcgcgagg accggcggtt gctgcgcagc atgggcgccg tgtgcagtca cggacatgac
    14101 gcaggtcggg ttctgatgcg atgcagcagc ggatcggcag gcagggccgt gcgggcggaa
    14161 agctggattg ccggcggctg ccttcgggcc ggatgatctt ttgataatta tttttatacc
    14221 atcatatttt gcttaaaccg gccttagtcc gtccgggcac tcatgcagcc catgcagcca
    14281 tttgcgcagg ccggggtccg agcgagcgtg ctgcaccgcg tgcagcggtt ccggagtgtc
    14341 cgatcatgca gtcccgccgc aatgcctcgc tgttcgtgcg cttcgcctcg gccaccgccc
    14401 gctgctcggg ccggccggtc accttcgtgc tcgcgttcct ggtcatcgtc gtctggggcg
    14461 tgaccgggcc gctgtttcat tacagcgata cctggcagct ggtcatcaac accggcacga
    14521 ccatcgtcac cttcctgatg gtgttcctga tccagaacac ccagaaccgc gacgccgagg
    14581 ccgtgcaggt caagctcgac gagctgatcc gcgccacgcg cggtgcgcac aacgcgctgc
    14641 tgaacctgga ggagctcgaa gaccgcgaca tcgagcgcat ccgcgtgcgc taccagcggc
    14701 tggccgagga ggcgagggcg gcgctgcgca gtgacggtgg cggcgatacc gacacgccgg
    14761 aagccggcga agacggggcg gcggccgacg gggggcgccg gcaggactga tgcgttgaaa
    14821 atattcgtcc cgtacctgtc tccgattccc gggggccggc gtatcggtca gcaggcagcc
    14881 cgcacgaggc cgccgctgac cgaactcccg cctccgagga tccgcacgat gaacatcctg
    14941 ctccgcagcc acgccgaccg tcgcctgccc gccctcaccc tcgcggccgc gctcggcctc
    15001 accctcgccg gctgcgccgg tgcgccggtc gccaccaccg gcagcgcgcc gctgaccgat
    15061 gccggcgggc gcacgctgta caccttcgac aaggacggcg acggcgtcag ccgctgcgcg
    15121 gcgacctgca ccgcgacctg gccgccgtat gtggtcaaag ccggtgaaaa cccggccggc
    15181 atgagcgcga tcacccgcga ggacggcagc cgccagtggt cgctggccgg ccggccgctg
    15241 tacttcttcg ccggcgacca gaagcccggc gatgcgcgcg gcgacggcgt cggcggcgtc
    15301 tggcacctcg cgcgcaatgc tgcgccggcg gcgagcgccg cgagcagcag cggcagcagc
    15361 tacggctact gagccggctg tcaggcgctg aggcgccggc ctgcacgagc gggtcttgcg
    15421 cgccccggat cgcggcgcag gcgcagcgat tcgatgacag aacacatcca gtcgacagcg
    15481 agccgggcct tcgtgcccga cccgggaggt ttcccatgtc cgcgattgcg ccttctgctg
    15541 cttcctgcac cagctcctca gaccgtttct caggccgctc cgccagccgt cgtccggcgc
    15601 gctggcggcg gcagctgctg ggggctgcgc tggccgcgac cgcggccgcc tcggccggtg
    15661 cgtcgccgct gctgttctcc ggcaccggtg ccgcggcgac gaccgccttc gatgccttcc
    15721 gcggcgcgat cggtggcggc agccgcatca cctgggacgg cgtgcgcctc gatggcagcg
    15781 acgtgaatcc ggcgacccag gtgctgatcc ccggcagcac ggtggcgatc cccgagaacc
    15841 gcttcaccgc cgccggggcg ctgttcgccg atccgtatgc ggtcgccggc gatggcttcg
    15901 ccgccgccaa cccggcaacc gccggccagt ttccggcctt cacgcccggc aacaccttcg
    15961 tgatgttcga taccgacggc agcgacttcg acgaccgctt catcgagcag agcttcgtgc
    16021 tgcccggcac gacgaccgcg gccggtacgc gcggcttcgg ggcgatcttc gtcggcgtcg
    16081 agcaggccgg cagcagcagc atcgagtact tcggccgcaa cgcgctcggc gagcgcatca
    16141 gcctcgggca ctttgcggtc gaggccggca gcgggccatc cgacacgcag ttcctcggcg
    16201 tgctgttcga tgcggcgatc gtcgccgacg tggtgctgac ggtcggcacg cacgcgctgt
    16261 tcggcttcga cggcacgacg ctgcgcagct tcggcgcgca gacgccgggg accgatctcg
    16321 ccgtcaccga cgacttcgtg ttcgcgcgcc ccgaggcgct ggtcacggtg ccggagccgc
    16381 cgccgctcgc gctcggcttc gcggccctcg gtgccgcgct gacgctgcgc cggctgcggc
    16441 cggccgactg aaagcctgct gtccgccgct cgccggccac gaccagcaac agcctgcggg
    16501 cggcgatctc ccgcgacagc ggccgcgctg cgcgcgccgc acatccctct tgacgaccgg
    16561 ccgggcaggg acccggccat cccgacttcc ggagttttca tcatgacgat gcaggatctg
    16621 cacggtcacg cgctcagcgg cgccagtgcc caggctctcg acgccttcga aatcgcgctg
    16681 cgcgaactgc gctgcctgag tggtgacccg ctcggccacg ccgaggcggc ggtcgcggcc
    16741 gcgccggagt tcacgatggc gcacgcgctg cgtgcctggc tgtttctggt cggcaccgac
    16801 gccggggccg tgccgctggc gcgcgaggcg ctggcgcagg cccgggccct gccggccgat
    16861 gcccgggagg cacggcatct ggcggcgatc gggcactggt gtgccggacg ctggcggctg
    16921 gcggcgcgca cgctcgaaga cctgagcctc gagcatccgc acgacctgct ggcgctgcag
    16981 ctcgggcagc agctcgactt cttcaccggc gaccagcgcc tgctgcggga tcgcatcgcc
    17041 cgggcgctgc cggcctggtc gccggacatg cccggctatc acgcgctgct cggcatgtat
    17101 gcgttcgggc tggaggagaa cggcgatttt cacaacgccg agcgctacgg ccgccgggcg
    17161 atcgaactgg agccgcgcga cgcctgggcg cagcacgcgg tcgtgcacgt gctggagatg
    17221 cagggccggc gcgatgacgg catccgctgg atgaccggtc agtccggctg gaagcagtgc
    17281 gggctgctcg ccgtgcacaa ctggtggcat ctggccctgc attacctcgg cctcgacgat
    17341 gtcgatgcgg cgctgcaact gctcgacggc ccgatcgacg ggcaccggcc gacgctggcg
    17401 ctggagctga tcgacgtgag cgcgctgctc tggcgcctgc gcctgcgcgg cgtcgatgtc
    17461 ggcgaacgct gggtgtcggt cgccgagcgc tggagcgcct gcgccgggca cggctggtat
    17521 gcgttcaacg acctgcacgc ggcgatggcc ttcgcctgtg ccggccgggc ggacctgctc
    17581 gaacagctcg acgccacgca gcgcgcggcg cagacgcgcg cagacgacaa cgccggcttc
    17641 ctgctggcgg tcgggcgggc ggcgaccgag gccgtgatcg cgcacgtcgc cggcgatcac
    17701 cggcgcacgg tcgagcggct gcgcgacctg cgcagccacg cccgcgcctt cggcggcagc
    17761 gatgcgcagc gcgacctgct cgaccagacg ctgatccact ccgcgcgcgc gagcggccag
    17821 ccggcgctgg cccgcggtct ggaagccgaa cgctcgctgc tggcccgcca gcgcgccggc
    17881 gccagacccg cggtggcgct gcaacctgca atgcactgat tcccctccgg aggaaggacc
    17941 ggggatgagg gggcggcatg gcacggaatc atggcgggtg atggtgttct gatcgctgcc
    18001 ctgcggaccg ggatgcctgc cgatcccggc cggcagcggg ca
//
LOCUS       DAOQIE010000031        17479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1508_length_17479_cov_7.418331, whole genome shotgun sequence.
ACCESSION   DAOQIE010000031 DAOQIE010000000
VERSION     DAOQIE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 17479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17479
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_17479_cov_7.418331"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..3483)
                     /locus_tag="PK144_03500"
     CDS             complement(28..3483)
                     /locus_tag="PK144_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNF64821.1"
                     /translation="MALAHVSLDDKYALESGRVYLNGTQALVRLLLMQRARDRLHGLN
                     TAGFVSGYRGSPLGGLDQALWGAKRFLERDGIVFKPGLNEDLAATAVWGTQQVGLFPG
                     ARHEGVFAMWYGKGPGVDRSGDVFKHGNLFGSAAKGGVLAIAGDDHASKSSTLPHQSE
                     YAFMDAMIPVLNPAGVQEILDLGVFGWELSRYCGAWVAMKTIAETVDSSASVTVDPER
                     MHIVLPEGFEFPPEGVHGRWPTRPLEQEFIHQKYRLYAALAFVRANRLDRIVLDSDAP
                     RFGIVTTGKSYLDVMQALDDLGIDAAEAGRIGLRVYKVGMSWPLERDGVRAFAQGLEE
                     ILVVEEKRAVIENQFKEQLYNWREDVRPRVIGKFDEQREWILPSTGELTPAQIARVIA
                     RRIGRFYTSKRIEQRLAFLEAKEAALAKPRPTLDRVPHFCSGCPHNTSTRVPEGSRAM
                     AGIGCHYMVTWMDRATDTFTQMGGEGVTWIGQAPFTDTPHVFQNLGDGTYFHSGLLAI
                     RAAIAAQVNITYKILYNDAVAMTGGQHVDGSLSVAQITHQLYGEGVRRLAVVSDEPGK
                     YRNRGAFADGVTFHHRDALDALQRELRSVPGTSVLIYDQTCAAEKRRRRKRGLMPDPP
                     RRVFINAAVCEGCGDCSVQSNCLSVVPLETGFGRKRAIDQSNCNKDYSCLNGFCPSFV
                     TVHGAKLRGNRTALAADDWSLALPEPGLPALTEPWGIVITGVGGTGVVTIGALLGMAA
                     HLEGKGVSVLDMTGLAQKYGAVVSHVRIAAQPEAIHAVRIAAGGARLLLGCDYVVAAG
                     SEALSKLDPQHSHAIINAHQAMPARFVREPDLNFPDEAMRTAIREAAGDAEFIDATAL
                     ATGLFGDALASNLFLLGFAWQRGRVPLSGEAILRAIELNGAAAEANRAAFLWGRRAAI
                     DEAAVRRRALPAAVSELKRAPALDEWIGQRAAFLGEYQDADWAARYRDLVARVRSAEA
                     GLGSETLTRTVAQVLFRLMSYKDEYEVARLYVDAGFRASLDAVFEPGYTLGFHLAPPL
                     FAKRDPHTGLPVKQTFGPWLMRVFGWLAQGRRLRGTWLDPFGWTAERRGERRLIGEYE
                     QLVDELLGGLSRERLTLAVEIAGLHGQVRGFGHVKARALAEMRGRLDERLGVWRRPQA
                     SARAA"
     gene            complement(3665..4801)
                     /locus_tag="PK144_03505"
     CDS             complement(3665..4801)
                     /locus_tag="PK144_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNF64822.1"
                     /translation="MATRLVAHVVHRLTVGGLENGVVNLINHLPADRFRHVVICMTDH
                     TGFAARIRRADVGIIDLHKRPGHDAGLYLRLYRTLRRLRPDVVHTRNLGTQEALAIAW
                     LAGVRGRVHGEHGWNGPGPEAEPPRRHRLRRWLRPFVSEYVALGAEIERYLQTRIGVP
                     AQRIARIYNGVDLTRFGPEPQDRRAVLPAGFAADDAVVIGTVGRMQNVKDQPTLARAF
                     VALLAAQPAWRARLRLVLVGDGELRSEVEAILAAAGARELAWLPGSRDDVPALLAAFD
                     VFVLPSQAEGVSNTILEAMACARPVVATAVGANAELLVDGETGRLVPARDPAAMADAL
                     AAYAASPALRAAHGAAGRARIEAQFSLEAMVARYAGVYERALAG"
     gene            complement(4819..6387)
                     /gene="xrtA"
                     /locus_tag="PK144_03510"
     CDS             complement(4819..6387)
                     /gene="xrtA"
                     /locus_tag="PK144_03510"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNF64823.1"
                     /translation="MSDPALVSVTAADAQAPLTDAAPVPARGWPVTLAALTVALLATI
                     GLYWPTATGMASIWWRSDTFAHGMLVVPIVLYMLWTRRAEIAPVAPRPAYPGLALLAL
                     LAAGWFIADSAGVGVGSQLCLVAMFPALVWTLCGRAVVAAAAFPLAYLFFAVPIGEGL
                     VPPLQDFTAHFTVRAVRLTGIPVYAEGWYLMLPSGTFQVAEACSGIRYLIASLALGCL
                     YAYLTYRSPWRRFLFIVFAAIVPVLANGLRAFGIVMIAHFTKMEYATGADHLVYGWLF
                     FGLVIGLMFWVGRFWEEDPSVPPVRQTGLTTAAPAPAAAAQPWPALVLSLCVLALAPL
                     ALWRSDAQPVASVPVLTAPAVAGWNGPKPAEWATTYTGQTRTLVARYDDGRGHDLRLW
                     VATWAGGHDSSRMISSVHLPYKPGEWVQGADRVRRVPTAAGEREVYEVPVIAGERRLT
                     IWTWYSVGGHPTVSPSTGKLWQAWNMLSGNHTPGRVFVLVTDGSDPPDVVDERLSGFL
                     GAAAQPLDRLGLAD"
     gene            complement(6384..7583)
                     /locus_tag="PK144_03515"
     CDS             complement(6384..7583)
                     /locus_tag="PK144_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNF64824.1"
                     /translation="MEELLLLVHRIPYPPNKGDKIRSYHLLRFLAARYRVHLGAFVDD
                     PADFAHATQLQAMCASLCLRPLDARRAKLRAFSGLLTGEALSLPYYRDRGFARWVDGV
                     IARGVRRVVVFSSAMAQYVLDRPGLELVTDFCDVDSDKWRQYAQVQRWPQSWVYRREA
                     ARLLAFDRRVAAASRAALFVSEPEAALFRQLAPEAAGHTGFFHNGVDTAYFDPATTYE
                     NPYPAGGRPLVFTGAMDYRANVDAVDWYARAVLPIVRAAQPDAQFWIVGSRPAAAVQA
                     LGALPGVSVTGAVADVRPYVAHAAAAVAPLRIARGVQNKVLEAMAMARPVLATTAAMD
                     GIVPNPRFDALVADEAAPLAAHTIRLLADGDRDGLGALGRAWVLAHYDWDTNLRPVAG
                     WLEREAA"
     gene            complement(7596..8660)
                     /locus_tag="PK144_03520"
     CDS             complement(7596..8660)
                     /locus_tag="PK144_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNF64825.1"
                     /translation="MSVAATMSTAAGSIAVRRLQPADHARWDAFVAACPEATFFHRAG
                     WAEVLQRAFGHDAHFLYAERDGVIVGVLPLGHLRSRLFGNALISVPFCVYGGIAATDE
                     EVRRPLADAACRLAQELGVDYLELRHRRRQFPDWPCKDGLYVTFRKPILPDEDANLKA
                     IPRKQRAMVRKGIEAGLEGRIEAGVDNLFLAYSESVRNLGTPVLPKRYFQVLREVFGS
                     DCEVLTIRQGETLVASVLSFYFRDEVLPYYGGGTAQAREVKGNDFMYWDLLRRSCARG
                     LRLFDYGRSKNGTGSYSFKKNWGFEPEPLYYEFHLVRATRLPDINPLNPKYRLFIAAW
                     QRLPLWLSRRLGPWLARSLG"
     gene            complement(8657..9514)
                     /locus_tag="PK144_03525"
     CDS             complement(8657..9514)
                     /locus_tag="PK144_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNF64826.1"
                     /translation="MQATRPVNAMTVDVEDWFQVSALTPWVRREDWETLPRRVGANTH
                     RVLDLFAGAGVRATFFTLGWVAERHPELVRRIVAEGHELASHGWQHVRVIHQSPEEFR
                     ADVIRTKALLEDLGGVPVRGYRAASYSIGARNLWALEILAETGHVYSSSIFPGSHDLY
                     GMPGAPRFRFRPDNAPALLEVPITTVELGTRRLPAAGGGFFRLFPYALSRALLRRVNR
                     EDRESGLFYFHPWEIDPAQPRPAGVSGRARLRHYLNLGRTESRLRRLLADFSWDRMDR
                     VFLDPESVA"
     gene            complement(9532..10620)
                     /locus_tag="PK144_03530"
     CDS             complement(9532..10620)
                     /locus_tag="PK144_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNF64827.1"
                     /translation="MYTQYYGFSAKPFQLSPDPRFFFGSSGHKRALAYLRYGLSQGEG
                     FIVVTGGIGTGKTTLVRNLFAELDPATVVAAQLVTTQLEADDLLRMVVASFGLAHESV
                     SKASLLKYFEDYLRSCAREGRRVLLIVDEAQNLPARSLEELRMLSNFQVGEKPLLQSF
                     LLGQDEFRATLGGPGLEQLRQRVIASCHLHPLGADETRQYIEHRLRVVGWQGRPAFTE
                     AAFRLIHETTQGIPRRINVFCDRVLLYGFLEELTEFGEEAVRATRSEFAQEVFANSAG
                     AARTAEAPAPRPVTDPAAGDALAARLDRIERAVSGLLQVQAGGEESAEAERRLGELEA
                     SVGMLKEGFLRLANVIDRHFETDRSEAS"
     gene            complement(10664..12313)
                     /locus_tag="PK144_03535"
     CDS             complement(10664..12313)
                     /locus_tag="PK144_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HNF64828.1"
                     /translation="MSKRTRQFGRGCLSLFVLVSGTAAAGDVTMQSSLSVSGTLTDNI
                     TRGQIQSDGSPVSKGDFITEIRPSIGGRSQGAGLKYDFNYELSAVEYFHDTARNAVNN
                     RFRLNGTAEVVEDRFLLDFRSAYFQVANAPSADTLNDPLLGSGGYSNVGTLTLTPIWR
                     EHLGSFADSEMTGTLGLTTTSDQSDNVGGASASASDSTVLGIDWRLSNGRSSGNTGWS
                     VVYSRSVEGYADGNLGNDEREHAEASVRQGISREWALLGRVGLENNSTHDISEQEAAN
                     GNYWSGGLGWTPSRVLGLDAWYGSRDKELTLNWNPSERTSLVLSWRDRAVGLVTGPSW
                     NLDFRHRTAYSDWTARYSEEVTNSQGVARRDSNFLSFVNPQTGEVVYFDPATGRLLPA
                     SSAFSLNNESFLRKRFSLGNSYQRGHSQFNSNVFHESREYEASGRKETSLGLDASWSW
                     QFAPRTRSVLAVNWERVSGDDSSSGSGSGSDSTGSSGDSDYLTGRIGLSHQLSADADA
                     LIEYAHTTSDGGSGGFGSSGGGSSSQSYDENRLTLSINMRF"
     gene            complement(12306..13232)
                     /locus_tag="PK144_03540"
     CDS             complement(12306..13232)
                     /locus_tag="PK144_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNF64829.1"
                     /translation="MDTIEKAIERLSRHERQPAPVAEAQGTAAIVIEPAPLPAVPGSQ
                     PVRAETPAAPAVVRRTKRSVTLDLQRIRDAGMVVPDGNRSRIKEEYRHIKRPLLLNAA
                     GKGAAVRPNQNVMMVSSSQPGEGKTFTSINLALSIAAERDRTVLLVDADVLKPTVSRF
                     FGVDNGPGLVDFLVDGQLDLSEVLVETNVPSLVLLPAGNSHHLSTELLGSTAMRDLMT
                     EMSNRYSDRVIIMDSPPLLATTEAAVLAHLVGQIIMVVEAERTRQSLVKEALALLDPD
                     MAVGFVLNKSRQAARGDYYGPYYYSYAEPARE"
     gene            complement(13254..14852)
                     /locus_tag="PK144_03545"
     CDS             complement(13254..14852)
                     /locus_tag="PK144_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HNF64830.1"
                     /translation="MHEIVEQILGYLRGMWRYRWYALAVAWTISLGGWFFVHRLPDQF
                     EASARVYVDTQSILRPLLRGLTVDVNPDSQIQLMTRTLLSRPNLEKVARMTDLDLKAR
                     DNAQMDVLLDRLGKHISLRSLGRENLYTIGFEDKDPGFAKKVVQSLLTIFVENALGET
                     RQDSDTAQRFLDKQIAEYEARLLAAEERLKNFKRQNVDMMPQSGQDHYSKMQEMGSQL
                     AAARLELKQIEQRRDTLKAQLDGDTATFGVGPKPVIAQEGPASITDSAPVDGRIKMME
                     ERLDQLLLQYTEKHPDVIAVRQTIADLRKQREKEIAEIRAATAAAAKAASSNGGEPVQ
                     DGNPYLQQVRMMLTAEEGNAAASRVRVEEFERRLAEMQRMVNSVPEVEAQLKALNRDY
                     DVTRQNYDQLLARRESARLSQQAGQTNEDIKFKVIDPPRVPNEPTGPNRVGLMSGVFG
                     GAVIGGIAVAFLISQLRPTFDSRRMLTDLTGLPVLGMVSLVVSPDTIRRQRYGLAGFV
                     GMGMLLVLTFGGLLAAQLTGHRLI"
     gene            complement(14895..15545)
                     /locus_tag="PK144_03550"
     CDS             complement(14895..15545)
                     /locus_tag="PK144_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016957068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HNF64831.1"
                     /translation="MIHVLAVPCRVCMVLVLASLAVLAGCSSTELPTLPTTGNYGTQT
                     TDPSKYNYIIGPGDSVQIFVWRNPEVSQSVTVRPDGKITTPLVEDLQASGRTPTQLAR
                     DIEKVLETYIRQPIVTVIMGGFGGPYSEQIRVVGEAAKPQSIPYREKMSLLDVMILVG
                     GLTQFADGNEAKIVRVVEGQQQQYRVRIDDLLKDGDITANVDMLPGDILIVPESWL"
     gene            15778..16476
                     /locus_tag="PK144_03555"
     CDS             15778..16476
                     /locus_tag="PK144_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64832.1"
                     /translation="MTAPSPLLQLRKVCRGYAEGHGQRPVLAGAELALLPGEVVALLG
                     ASGSGKSTLLNLAAGIDLPDAGEVCFDGRDLARLSETARTRLRRRQVGIVFQFFNLVP
                     TLTVAENARLPLELNGLADAAGLARVAALLDAVGLADAARRWPGELSGGEQQRVALVR
                     ALVHRPKLLLADEPTGALDAENGARVLDLLLRLAREHAATVLIVTHAAEVAAAADRRL
                     RLADGRLHAVDGAA"
     gene            16476..>17479
                     /locus_tag="PK144_03560"
     CDS             16476..>17479
                     /locus_tag="PK144_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64833.1"
                     /translation="MPARLLLAAALRELARQPLQFALAVLGIALGVAVVLAVRLATAS
                     ALDGFERAADALGGRATHALLAGPAGVPDDAWRWLRIEAGVRSAAPLVQGWVERTDAP
                     GRWLQLIGVDPWAEAPLARGGARLGGWPAQLTTQPDAVLASSALARRLQLAVGERLTI
                     RAGGRTQSLRIAGRFEADDDAAAERLIADIATAQAVLGRAGRLDRIDLVLDDAAAAAL
                     AARLPPPLRLETAAARAGELGRLTAAFRFNLDALSLLALLVGAFLIRNTLEFAVVRRR
                     PLLGLLRALGVTRRELLGLIALEAAVLGLLGSALGLLLGALLARGLIGLVGRTLAEVY
                     "
BASE COUNT         2572 a         6099 c         6216 g         2592 t
ORIGIN      
        1 cggcggagcg gcatgagcgg cgcggactca ggccgcccgc gcgctggcct gcggccggcg
       61 ccagacgccg aggcgctcat cgaggcgccc gcgcatttcg gccagcgccc tggccttgac
      121 gtggccgaag ccccggacct gaccgtgcag cccggcgatc tcgacggcga gcgtcaggcg
      181 ctcccggctc agcccgccca gcagttcgtc gacgagctgt tcgtactcgc cgatcagccg
      241 gcgctcgccg cgccgttcgg cggtccagcc gaagggatcg agccaggtgc cgcgcagcct
      301 ccggccctgc gcgagccagc cgaacacgcg catcagccac ggcccgaagg tctgcttcac
      361 cggcaggccg gtgtgcggat cgcgcttcgc gaacagcggt ggcgcgaggt ggaaaccgag
      421 cgtgtagccg ggctcgaaca cggcatcgag gctggcgcgg aagccggcat cgacgtacag
      481 gcgcgcgacc tcgtattcgt ccttgtaact catgagccgg aacagcacct gcgcgacggt
      541 cctcgtcagc gtctcgctgc cgaggccggc ttcggcgctg cgcacgcgag cgacgaggtc
      601 gcggtagcgc gcggcccagt ccgcatcctg gtattcgccg agaaaggccg cgcgctgtcc
      661 gatccactcg tcgagcgccg gcgcgcgctt cagctcgctg accgccgccg gcagcgcacg
      721 ccggcgcacc gcagcctcgt cgatcgcggc gcgccgcccc cacaggaacg cggcccggtt
      781 ggcctcggcc gccgcgccgt tgagctcgat cgcgcgcagg atcgcctccc ccgacagcgg
      841 cacgcgcccg cgctgccagg cgaagccgag caggaacagg ttgctcgcca gcgcatcgcc
      901 gaacaggccg gtggcgagcg cggtcgcatc gatgaactcg gcatcgccgg cggcctcgcg
      961 gatcgccgtg cgcatggcct cgtccgggaa gttcaggtcc ggctcgcgca cgaagcgcgc
     1021 cggcatcgcc tgatgggcat tgatgatcgc gtgcgagtgc tgcggatcga gtttggagag
     1081 cgcctcgctg ccggcggcga cgacgtagtc gcagccgagc agcagccggg cgccgccggc
     1141 ggcgatgcgc acggcgtgga tggcttcggg ctgcgcggcg atgcgcacgt gcgagaccac
     1201 ggcgccgtac ttctgcgcga gcccggtcat gtcgagcacg ctcacgccct tgccttcgag
     1261 gtgcgcagcc atgccgagca gcgcgccgat ggtcacgaca ccggtgccgc cgacgccggt
     1321 gatgacgatg ccccagggct cggtgagcgc cggcaggccg ggttcgggca gcgccagcga
     1381 ccagtcatcg gccgccagcg ccgtgcggtt gccgcggagc ttcgcgccgt gcacggtcac
     1441 gaagctcggg cagaagccgt tcaggcagga gtagtccttg ttgcagttcg actggtcgat
     1501 cgcgcgcttg cgaccgaatc cggtctcgag cggcacgacc gacaggcagt tcgactgcac
     1561 gctgcagtcg ccgcagccct cgcagaccgc cgcgttgatg aacacccgcc gcggcggatc
     1621 cggcatcagc ccgcgcttgc gccggcggcg cttctcggcc gcgcaggtct ggtcgtagat
     1681 cagcacgctg gtgccgggca cgctgcgcag ctcgcgctgc agcgcatcga gcgcatcgcg
     1741 gtggtggaag gtgaccccgt cggcgaaggc accgcggttg cggtacttgc cgggctcgtc
     1801 ggagaccacg gcgaggcggc gcacgccctc gccgtatagc tggtgcgtga tctgcgcgac
     1861 gctcagcgag ccgtcgacgt gctggccgcc ggtcatcgcg accgcatcgt tgtagaggat
     1921 cttgtaggtg atgttgacct gcgcggcgat cgcggcgcgg atcgcgagca ggcccgagtg
     1981 gaagtaggtg ccgtcgccga ggttctggaa cacgtgcggc gtgtcggtga acggcgcctg
     2041 cccgatccag gtcacgccct ccccgcccat ctgcgtgaag gtgtcggtcg cacggtccat
     2101 ccacgtgacc atgtagtgac agccgatgcc ggccatcgcc cggctgcctt cgggcacgcg
     2161 cgtcgaggtg ttgtgcgggc agcccgagca gaagtgcggc acgcgatcga gcgtcggccg
     2221 cggtttcgcg agcgcggcct ccttcgcctc caggaaggcc agccgctgct cgatgcgctt
     2281 cgaggtgtag aagcgcccga tgcgccgcgc gatcacgcgc gcgatctgcg ccggcgtcag
     2341 ctcgccggtc gagggcagga tccattcgcg ctgctcgtcg aacttgccga tcacgcgcgg
     2401 gcgcacgtcc tcgcgccagt tgtagagctg ctccttgaac tggttctcga tgaccgcgcg
     2461 cttttcctcg acgaccagga tctcctccag cccctgcgca aacgcgcgca cgccgtcgcg
     2521 ctccagcggc cagctcatgc cgaccttgta gacgcgcagg ccgatgcgcc cggcctcggc
     2581 cgcgtcgatg ccgagatcat cgagcgcctg catcacgtcg agatagctct tgccggtcgt
     2641 gacgatgccg aagcgcggcg cgtcgctgtc gagcacgatg cgatcgagcc ggttggcgcg
     2701 cacgaaggcg agcgccgcgt acagccggta cttctggtgg atgaactcct gctccagcgg
     2761 ccgggtcggc cagcgcccgt gcacgccctc gggcgggaat tcaaagccct ccggcagcac
     2821 gatgtgcatg cgctccggat cgacggtgac gctcgccgac gagtcgaccg tctcggcgat
     2881 ggtcttcatc gccacccagg cgccgcagta gcgcgacagc tcccagccga acacaccgag
     2941 atcgaggatc tcctgcacgc cggccggatt cagcaccggg atcatcgcgt ccatgaacgc
     3001 gtattcgctc tggtgcggca gcgtcgagga cttcgaggcg tgatcgtcgc cggcgatcgc
     3061 gagcacgccg cccttggcgg cgctgccgaa caggttgccg tgcttgaaca cgtcgccgga
     3121 acggtccacc cccggcccct tgccgtacca catcgcgaac acgccctcgt gcctcgcgcc
     3181 cggaaacagc ccgacctgct gcgtgcccca gaccgcggtc gcggccagat cctcgttcag
     3241 cccgggcttg aacacgatgc cgtcgcgttc cagaaaccgc ttcgcgcccc agagcgcctg
     3301 atcgaggccg ccgagcggcg agccgcggta gccggagacg aagccggcgg tgttcaggcc
     3361 gtgcaggcgg tcgcgggcgc gctgcatcag cagcaggcgc accagggcct gggtgccgtt
     3421 cagatacacg cgccccgatt cgagcgcgta cttgtcgtca agactgacgt gggcgagggc
     3481 catgcgggga tctcccatct cggcttcaag cggtgccggc tgcgcatcct gcgggcgacg
     3541 gtgggtcgcg cgggacgcgg acggggcagc cggtgcgtac aggcggtcag acccttgtgg
     3601 ggcgagcctg ttgccgaggg taggaaggcc ggatcagccg gtcaatggtc agaccggtcg
     3661 cgcttcagcc ggccagcgcg cgctcgtaga cgcccgcgta gcgcgcgacc atcgcctcca
     3721 ggctgaactg cgcctcgatg cgcgcccggc cggcggcccc gtgggcagcg cgcagcgccg
     3781 gcgaggccgc ataggcggcc agcgcgtccg ccatcgccgc cgggtcgcgc gccggcacca
     3841 gccggccggt ctccccgtcg acgagcagtt cggcgttggc cccgaccgcg gtagcgacca
     3901 ccggtcgcgc gcaggccatg gcttcgagga tggtgttgga gacgccctcg gcctgcgagg
     3961 gcagcacgaa cacgtcgaag gccgcgagca gcgccggcac gtcgtcgcgg ctgccgggca
     4021 gccaggcaag ctcacgcgcg ccggcggccg cgaggatggc ctcgacctcg ctgcgcagct
     4081 cgccgtcgcc gaccagcacc agccgcagcc gcgcccgcca tgccggctgc gcggcgagca
     4141 gggcgacgaa ggcgcgcgcg agcgtcggct ggtccttgac gttctgcatg cgcccgaccg
     4201 tgccgatgac gacggcatcg tcggccgcga agccggccgg cagcacggcg cggcgatcct
     4261 gcggttcagg accgaagcgc gtcaggtcga cgccgttgta gatgcgggcg atgcgctgcg
     4321 ccggcacgcc gatgcgcgtc tgcagatagc gctcgatctc ggccccgagc gcgacgtatt
     4381 cgctgacgaa gggccgcagc cagcggcgca gccggtgccg gcgcggcggc tcggcctcgg
     4441 ggccggggcc gttccagccg tgctcgccgt gcacgcgccc gcgcacgccg gcgagccagg
     4501 cgatggcgag ggcctcctgc gtgccgaggt tgcgcgtgtg cacgacatcg ggccgcaggc
     4561 ggcgcagcgt ccggtacagg cgcaggtaca ggcccgcatc gtggccgggg cgcttgtgca
     4621 ggtcgatgat cccgacgtcg gcgcggcgga tgcgcgcggc gaagccggtg tgatcggtca
     4681 tgcagatcac cacgtgccgg aagcgatcgg ccggcaggtg gttgatcagg ttgacgacgc
     4741 cgttttccag gccgccgacc gtcagccggt gtacgacgtg ggcgaccagt cgcgttgcca
     4801 ccagagccgc gcgccccgtc agtccgccag accgagccgg tcgagcggct gggcggcggc
     4861 accgagaaag ccgctcagac gctcgtcgac cacgtcgggc ggatcggaac cgtcggtgac
     4921 cagcacgaac acgcggcccg gcgtgtggtt gccgctcagc atgttccagg cctgccacag
     4981 cttgccggtg ctcgggctga cggtcgggtg accgccgacc gaataccagg tccagatcgt
     5041 cagcctgcgc tcgccggcga tgaccggcac ctcgtagacc tcacgctcgc cggccgccgt
     5101 cggcacgcgc cgcacgcgat cggcgccctg cacccattcg ccgggcttgt acggcaggtg
     5161 gaccgaggaa atcatccggc tgctgtcgtg gccgccggcc caggtcgcca cccacagccg
     5221 caggtcgtgg ccgcgcccat cgtcgtaccg cgccacgagc gtgcgggtct ggccggtata
     5281 ggtcgtcgcc cactcggccg gcttcgggcc gttccagccg gccacggccg gcgccgtcag
     5341 caccggcacg gacgccaccg gctgcgcatc gctgcgccag agcgccagcg gtgcgagcgc
     5401 aagcacgcag aggctcagca ccagcgccgg ccacggctgc gcagccgccg ccggtgccgg
     5461 cgctgccgtc gtcagaccgg tctgccggac gggcggcacg gacgggtcct cctcccagaa
     5521 gcgtccgacc cagaacatca ggccgatcac gaggccgaag aacagccagc cgtagaccag
     5581 atggtcggcg ccggtcgcgt actccatctt cgtgaagtgc gcgatcatga cgatgccgaa
     5641 ggcccgcagg ccgttggcaa gcaccggcac gatggccgcg aacacgatga acaggaagcg
     5701 ccgccacggc gagcggtagg tcaggtaggc gtacaggcag ccgagcgcga gcgaggcgat
     5761 caggtagcgg atgccgctgc aggcctcggc gacctggaag gtgccgctcg gcagcatcag
     5821 gtaccagcct tccgcgtaca ccgggatgcc ggtcaggcgc accgcccgca ccgtgaaatg
     5881 cgccgtgaag tcctgcagcg gcgggaccag gccttcgccg atcggcaccg cgaagaacag
     5941 ataggcgagc gggaacgccg ctgccgcgac cacggcccgg ccgcacagcg tccagaccag
     6001 cgccggaaac atcgcgacca gacagagctg gctgccgacg ccgacgccgg cgctgtcggc
     6061 gatgaaccag ccggcggcaa gcagcgcgag cagcgcgagc cccggatacg ccggccgcgg
     6121 tgccaccggc gcgatctcgg cacgccgcgt ccagagcatg tacagcacga tcggcacgac
     6181 cagcatgccg tgcgcgaagg tgtccgagcg ccaccagatc gaggccatgc cggtcgccgt
     6241 cggccagtac aggccgatgg tcgcgagcag ggccacggtc agcgccgcca gcgtcaccgg
     6301 ccagccgcgg gccgggaccg gagctgcatc cgtcagcggc gcctgcgcat cggcagcggt
     6361 gacgctgacc agagccggat cgctcatgcg gcctcccgtt cgagccagcc ggccaccggc
     6421 cggaggttgg tgtcccagtc gtagtgcgcg agcacccagg cgcgcccgag cgcgccgagg
     6481 ccgtcgcggt cgccgtcggc gagcagccgg atcgtgtgcg ccgcgagcgg cgcggcctca
     6541 tcggccacca gcgcgtcgaa gcgcgggttc ggcacgatgc cgtccattgc cgccgtcgtc
     6601 gccagcaccg gcctggccat cgccatcgct tcgagcacct tgttctgcac gccgcgcgcg
     6661 atgcgcagcg gcgcgaccgc cgccgcagca tgcgcgacat aaggccgcac gtcggccacc
     6721 gcgccggtca cgctcacgcc cggcagcgcg ccgagcgcct gcacggctgc ggccggtcgg
     6781 ctgccgacga tccagaactg cgcgtccggc tgcgcggcgc gcacgatcgg cagcaccgcg
     6841 cgcgcgtacc agtcgacggc atcgacgttg gcgcggtaat ccatcgcgcc ggtgaacacc
     6901 agcggccggc cgccggccgg atacgggttc tcgtatgtcg tggccggatc gaagtacgcg
     6961 gtatcgacgc cgttgtggaa gaagccggtg tggccggccg cctccggggc gagctgccgg
     7021 aacagcgccg cctcgggctc ggacacgaac agcgccgcgc ggctcgccgc ggcgacgcgc
     7081 cggtcgaagg cgagcaggcg cgcggcttcg cgccggtaga cccagctctg cggccagcgc
     7141 tgcacctgcg cgtactggcg ccacttgtcg gaatcgacat cgcagaagtc ggtgacgagt
     7201 tcgagcccgg gcctgtcgag cacgtactgc gccatcgccg acgagaacac gacgacacga
     7261 cgcacgccgc gggcgatcac gccgtcgacc cagcgggcga agccgcggtc acggtaatac
     7321 ggcagcgaca gcgcctcgcc ggtgagcagc ccgctgaatg cacgcagctt cgcgcgccgg
     7381 gcatcgagcg ggcgcaggca gaggctcgcg cacatcgcct gcagctgcgt ggcgtgcgcg
     7441 aagtcggccg gatcgtcgac gaaggccccg agatgcacgc ggtagcgcgc cgccaggaaa
     7501 cgcagcaggt gataggagcg gatcttgtcg cccttgttcg gcggatacgg gatgcggtgc
     7561 accagcagca ggagctcttc catgcccgtt cccgttcagc cgaggctgcg cgccagccag
     7621 ggcccgagcc ggcggctcag ccacagcggc aggcgctgcc aggcggcgat gaacagccgg
     7681 tatttcgggt tcagcggatt gatgtccggc agccgcgtcg cccgcaccag atggaattcg
     7741 tagtacagcg gctcgggctc gaagccccag ttcttcttga agctgtacga accggtgccg
     7801 ttcttgctgc gcccgtagtc gaacaggcgc aggccgcgcg cgcagctgcg ccgcagcaga
     7861 tcccagtaca tgaagtcgtt gcccttgacc tcgcgcgcct gcgcggtgcc gccgccgtag
     7921 tacggcagca cctcgtcgcg gaagtagaag ctcagcacgc tcgcgaccag cgtctcgccc
     7981 tggcggatgg tcagcacctc gcagtcgctg ccgaacacct cgcgcagcac ctggaagtag
     8041 cgcttcggca gcaccggcgt gccgaggttg cgcacgctct cggagtaggc gaggaacagg
     8101 ttgtcgacgc cggcctcgat gcggccttcg agcccggcct cgatgccctt gcgcaccatc
     8161 gcccgctgct tgcgcgggat cgccttcagg ttggcgtcct cgtcgggcag gatcggtttg
     8221 cggaaggtca cgtacaggcc gtccttgcac ggccagtccg ggaactggcg gcggcggtgg
     8281 cgcagttcga ggtaatcgac gccgagctcc tgcgcgagcc ggcaggcggc atcggcgagc
     8341 ggccgacgca cctcctcgtc ggtcgcggcg atgccgccgt agacgcagaa cggcaccgag
     8401 atcagcgcat tgccgaacag gcggctgcgc aggtgcccga gcggcagcac gccgacgatg
     8461 acgccgtcgc gctcggcgta caggaagtgc gcgtcgtgac cgaaggcacg ctgcaggacc
     8521 tcggcccagc cggcccggtg aaagaaggtc gcctccggac aggcggcgac gaaggcatcc
     8581 cagcgggcgt gatcggcggg ctgcaggcgg cgcacggcga tgctgcccgc agcggtgctc
     8641 atggtggcag cgacgctcat gcgacggact ccgggtcgag gaagacgcga tccatccggt
     8701 cccagctgaa atcggcgagc aggcgccgca gccgggactc ggtgcgaccg aggttcaggt
     8761 agtgccgcaa gcgcgcgcgc ccggacacgc cggccggccg cggctgcgcc ggatcgattt
     8821 cccaggggtg gaagtagaac agcccggact cgcgatcctc gcgattgacc cgccgcagca
     8881 gcgcacgcga cagcgcatac gggaacagcc ggaagaaacc gccgccggcc gccggcagcc
     8941 ggcgcgtgcc gagctcgacc gtcgtgatcg gcacctccag cagcgccggc gcgttgtcgg
     9001 gccggaagcg gaagcgcggc gcaccgggca tgccgtagag gtcgtgactg cccgggaaga
     9061 tgctggagct gtagacgtgg ccggtctcgg cgaggatttc gagcgcccac aggttgcgcg
     9121 ccccgatcga gtagctcgcg gcgcggtagc cgcgcaccgg cacgccgccg aggtcttcga
     9181 gtagggcctt cgtgcggatc acgtcggcgc ggaattcctc cgggctctgg tggatcacgc
     9241 gcacgtgctg ccagccgtgg ctcgccagtt catggccttc ggcgacgatg cggcgcacga
     9301 gctcggggtg acgctcggca acccagccga gcgtgaagaa ggtcgcccgc acgccggcac
     9361 cggcgaacag gtcgagcacg cggtgcgtgt tggcgccgac gcgccgcggc agggtttccc
     9421 agtcctcgcg ccgcacccag ggcgtgagcg ccgagacctg gaaccagtcc tcgacatcga
     9481 ccgtcatcgc gttgacggga cgggtggctt gcatcggcgg tctccgcagg ctcaggaggc
     9541 ttcggaccgg tcggtctcga agtgccggtc gatgacgttc gcaagacgca ggaagccttc
     9601 cttgagcatg ccgacgctcg cttcgagctc gccgagccgg cgctcggcct cggccgattc
     9661 ctcgccgccg gcctgcacct gcagcaggcc gctgacagcg cgctcgatgc gatcgaggcg
     9721 tgccgccagc gcatcgccgg ccgccgggtc cgtcaccggt cgcggggccg gggcctcggc
     9781 cgtgcgggca gccccggcgc tgttcgcgaa cacctcctgc gcgaactcgc tgcgcgtggc
     9841 gcgaacggct tcttccccga actcggtgag ttcctccagg aagccgtaga gcagcacgcg
     9901 atcacagaac acgttgatgc gccgcggaat gccctgcgtg gtctcgtgga tcagccggaa
     9961 ggcggcctcg gtgaaggccg gccggccctg ccagccgacc acgcgcagac ggtgctcgat
    10021 gtactggcgg gtctcgtcgg ccccgagcgg gtgcaggtgg caggaggcga tcacgcgctg
    10081 gcgcagctgt tcgaggccgg ggccgccgag cgtggcgcgg aactcgtcct ggccgagcag
    10141 gaagctctgc agcagcggtt tctcgccgac ctggaagttc gacagcatgc gcagctcctc
    10201 cagcgagcgc gccggcaggt tctgcgcctc gtcgacgatc agcagcacgc gccggccctc
    10261 gcgcgcacac gagcgcaggt agtcctcgaa gtacttcagc agcgaggcct tggacaccga
    10321 ttcgtgcgcg agcccgaagg acgcgaccac catgcgcagc aggtcgtcgg cttcgagctg
    10381 ggtcgtgacc agctgcgcgg cgacgacggt ggccgggtca agctcggcga acaggttgcg
    10441 caccagcgtc gtcttgccgg tgccgatgcc gccggtgacg acgatgaagc cctcgccctg
    10501 cgacagaccg tagcgcagat aggcgagcgc acgcttgtgg ccgctgctgc cgaagaagaa
    10561 gcgcggatcg ggactgagct ggaagggttt ggcgctgaag ccgtagtact gcgtgtacac
    10621 gcgtctcgat tcccgtgatg cggcgcaagc cggggcaggc gcttcagaag cgcatgttga
    10681 tgctgagggt cagccggttc tcgtcgtagg actgcgagct gctgccaccg ccgctgctgc
    10741 cgaaaccgcc gctgccgccg tcgctggtgg tgtgggcgta ttcgatcagc gcatcggcat
    10801 cggccgagag ctggtgactg aggccgatgc ggccggtcag atagtcgctg tcgccggaac
    10861 tgccggtcga gtcggaaccg gagccggaac cggaactgct gtcgtcgccg gacacgcgtt
    10921 cccagttgac ggcaagcacg ctgcgggtac gcggtgcgaa ctgccagctc cagctggcat
    10981 cgaggccgag ggaggtttcc ttgcgtcccg aggcttcgta ttcgcgactc tcgtgaaaga
    11041 cgttggaatt gaactggctg tggccgcgct ggtagctgtt gccgagcgag aagcgcttgc
    11101 gcaggaagga ttcgttgttc agcgagaacg ccgacgatgc cggcagcagc cgcccggtgg
    11161 ccggatcgaa atagacgacc tcgccggtct gcggattcac gaacgacagg aagttgctgt
    11221 ccctgcgcgc gacgccctgc gagttcgtga cttcctcgga atagcgcgcg gtccagtccg
    11281 aataagccgt gcggtgacgg aaatcgagat tccagctcgg tccggtcacg aggccgacgg
    11341 cacgatcccg ccagctcagc accagtgagg tgcgctcgga cggattccag ttcagcgtca
    11401 gttccttgtc gcgcgagcca taccaggcgt cgaggcccag cacgcgtgac ggcgtccagc
    11461 cgaggccacc gctccagtag ttgccgttgg cggcttcctg ttcgctgatg tcatgggtcg
    11521 agttgttttc cagaccgacc cggccgagca gcgcccattc gcgactgatg ccctggcgca
    11581 cgctcgcttc ggcgtgctcc cgctcgtcgt tgccgagatt gccatcggcg taaccttcga
    11641 ccgagcgcga atagacgacg ctccagccgg tgttgccgct gctgcgaccg ttgctgagcc
    11701 gccagtcgat gccgagcacg gtcgaatccg aagcgctggc cgaggcgccg ccgacgttgt
    11761 cgctctggtc ggaggtcgtc gtcagaccga gcgtgccggt catttcggag tcggcgaagc
    11821 tgccgaggtg ctcgcgccag atcggcgtca gcgtcagcgt gccgacgttc gaatacccgc
    11881 ccgaaccgag cagcggatcg ttcagggtat cggccgacgg cgcattggcg acctggaagt
    11941 acgcggagcg gaaatcgagc aggaagcggt cctcgacaac ttcggccgtg ccgttgagcc
    12001 ggaagcggtt gttgaccgca ttgcgggccg tgtcgtggaa atactccacg gccgacagct
    12061 cgtagttgaa atcgtacttg aggccggcgc cctggctgcg cccgccgatc gaaggccgga
    12121 tctcggtgat gaagtcgccc ttcgagaccg ggctgccatc cgactggatc tggccgcgcg
    12181 tgatgttgtc cgtcagcgtg ccgctgacgc tcaggctgga ctgcatcgtc acgtcgccgg
    12241 cggcggcggt ccccgatacc agcacgaaca gggacaggca gccccggccg aactgccggg
    12301 tccgcttact cacgcgccgg ctccgcgtag ctgtagtagt acgggccgta gtagtcgccg
    12361 cgggcggcct ggcgcgactt gttcagcacg aagccgaccg ccatgtccgg atcaagcagc
    12421 gccagcgcct ccttgaccag cgactgacgc gtgcgctcgg cctcgacgac catgatgatc
    12481 tggccgacca gatgcgcgag taccgcggct tcggtggtgg cgagcagcgg cggcgaatcc
    12541 atgatgatga cgcggtccga atagcggttg ctcatctcgg tcatcaggtc gcgcatcgcc
    12601 gtgctgccga gcagctcggt cgacagatgg tgcgagttgc cggccggcag cagcaccagc
    12661 gacggcacgt tggtctcgac caggacttcg gacaggtcga gctgaccatc gaccaggaag
    12721 tcgaccaggc ccgggccgtt gtcgacgccg aagaagcgcg acaccgtcgg cttcagcacg
    12781 tcggcatcga cgagcagcac ggtgcggtcg cgctcggcgg cgatgctgag cgccaggttg
    12841 atcgacgtga aggtcttgcc ctcgcccggc tgcgagctcg acaccatcat gacgttctgg
    12901 ttggggcgca ccgccgcgcc cttgccggcg gcgttgagca gcagcggccg cttgatgtgc
    12961 cggtattcct ccttgatgcg gctgcggttg ccgtccggaa cgaccatgcc ggcatcgcgg
    13021 atgcgctgca ggtcaagcgt gaccgaacgc ttggtgcgcc ggaccacggc cggtgcagcc
    13081 ggggtttcgg cacgcaccgg ctggctgccg ggcactgccg gcagcggggc cggctcgatg
    13141 acgatggccg cggtgccctg cgcctcggcg acgggagccg gctgccgttc gtggcggctc
    13201 aggcgttcga tggctttttc gatggtgtcc aagaacgagc gctccaggcg gcgtcagatc
    13261 agccggtggc cggtcagctg ggcggccagc agtccgccga aggtcagcac cagcagcatg
    13321 cccatgccga cgaaacccgc gagcccgtag cgctggcgcc ggatcgtgtc gggcgagacg
    13381 accaggctga ccatgccgag caccggcagg ccggtcagat cggtcagcat gcgccggctg
    13441 tcgaaggtcg gacgcagctg cgagatcagg aaggccacgg cgatgccgcc gatgaccgcg
    13501 ccgccgaaca cgcccgacat cagcccgacg cggttcggac cggtcggctc gttcggcacg
    13561 cgcggcggat cgatgacctt gaacttgatg tcctcgttgg tctggccggc ctgctgcgac
    13621 aggcgcgccg attcgcggcg ggcgagcagc tgatcgtagt tctggcgggt cacgtcgtaa
    13681 tcgcggttca gcgccttcag ctgcgcctcg acctcgggga cgctgttgac catgcgctgc
    13741 atctcggcca ggcggcgctc gaactcctcg acgcgcacgc gcgaggcggc ggcattgccc
    13801 tcctcggcgg tcagcatcat gcgcacctgc tgcagataag gattgccgtc ctggaccggc
    13861 tcgccgccgt tgctgctggc cgccttggcc gcggccgcgg tggcggcacg aatctcggcg
    13921 atttccttct cgcgctgctt gcgcaggtcg gcgatggtct ggcgcacggc gatgacgtcg
    13981 ggatgcttct cggtgtactg cagcagcagc tggtcgagtc gctcctccat catcttgatg
    14041 cggccgtcga ccggtgccga atcggtgatg ctggccgggc cttcctgcgc gatcaccggc
    14101 ttcggaccga cgccgaaggt ggccgtgtca ccgtcgagct gcgccttcag cgtgtcgcgc
    14161 cgctgctcga tctgtttcag ttcgaggcgt gcggccgcga gctgcgagcc catctcctgc
    14221 atcttgctgt aatggtcctg accggactgc ggcatcatgt cgacgttctg gcgcttgaag
    14281 ttcttcaggc gctcctcggc cgcgagcagg cgtgcctcgt attcggcgat ctgcttgtcg
    14341 aggaagcgct gcgcggtgtc ggagtcctgc cgggtctcgc cgagcgcgtt ctcgacgaag
    14401 atcgtcagca gcgactgcac gaccttcttc gcgaagcccg gatccttgtc ctcgaaaccg
    14461 atcgtgtaga ggttctcgcg cccgaggctg cgcagcgaga tgtgcttgcc gaggcgatcg
    14521 agcagcacgt ccatctgcgc gttgtcgcgg gccttcaggt cgaggtcggt catgcgcgcg
    14581 accttctcga ggttcggccg gctgagcagc gtgcgcgtca tcagctggat ctgcgaatcc
    14641 gggttcacgt cgacggtcag gccgcgcagc agcgggcgca ggatcgactg cgtgtcgacg
    14701 tagacacgcg ccgaggcctc gaactggtcg ggcaggcggt gcacgaagaa ccagccgccg
    14761 aggctgatgg tccaggcgac ggccagcgcg taccagcggt aacgccacat gccgcgcagg
    14821 taaccgagga tttgttcgac gatctcgtgc atgtcggaaa ccgtggttct gcgtggttct
    14881 tcgtggatct gcgcttacag ccaggattcc ggaacgatca ggatgtcgcc cggcagcatg
    14941 tcgacgttgg cggtgatgtc gccgtccttc agcaggtcgt cgatgcgcac gcggtactgc
    15001 tgctgctgac cctcgacgac gcgcacgatc ttggcctcgt tgccatcggc gaactgcgtc
    15061 aggccgccga cgaggatcat cacgtcgagc agcgacatct tctcgcggta cgggatcgac
    15121 tgcggcttgg ccgcctcgcc gaccacgcgg atctgctcgg agtacgggcc gccgaagccg
    15181 cccatgatca cggtcacgat cggctggcgg atgtaggttt ccagcacctt ctcgatgtcg
    15241 cgtgccagct gcgtcggcgt gcggccgctg gcctgcaggt cctcgaccag cggcgtcgtg
    15301 atcttgccgt ccgggcgcac ggtgacgctc tgcgagacct cggggttgcg ccagacgaag
    15361 atctgcaccg agtcgccggg accgatgatg tagttgtact tggacggatc ggtggtctgc
    15421 gtgccgtaat tgccggtggt cggcagcgtc ggcagttcgg tgctgctgca gccggcgagc
    15481 accgcgaggc tcgcgagcac gagaaccatg cacacgcggc acggcacggc aaggacatga
    15541 atcatggaaa ttgctccgtc cggtccgaaa tttgcgtatt tatcccgtaa accgcgtcgg
    15601 tgcgccccct gcacctgcgg gtggacccga ttcggcgatt atcccggagg cggcgcaagc
    15661 gtgtcgatgc agccatgtca ttgacgggtc aattgttgtc acggtcggca cggtgtgtgc
    15721 gtgcggcgtc cgcggcggca tcggccacga ttctgcacga aatgcacggg taatggcatg
    15781 accgctccgt caccgttgct gcagctgcgc aaggtctgtc gcggttacgc cgaaggccac
    15841 ggccagcgtc cggtgctcgc cggtgccgaa ctggcgctgc tgcccggcga ggtggtcgcg
    15901 ctgctcgggg cctcgggcag cggcaagtcg acgctgctga acctcgccgc cggcatcgac
    15961 ctgcccgatg ccggcgaggt gtgcttcgac ggccgcgacc tggcgaggct ctccgagacc
    16021 gcccgcacgc ggctgcgccg ccggcaggtc ggcatcgtgt tccagttctt caatctggtg
    16081 ccgacgctga cggtcgccga gaacgcccgc ctgccgctgg aactgaacgg cctcgccgat
    16141 gccgcggggc tggcgcgcgt cgcggcgctg ctcgatgccg tcgggcttgc cgatgccgcc
    16201 cggcgctggc ccggtgaact ctccggcggc gagcagcagc gcgtggcgct ggtgcgcgcg
    16261 ctcgtgcacc ggccgaagct cctgctcgcc gacgagccga ccggcgcgct cgatgccgaa
    16321 aacggcgcgc gcgtgctcga cctgctgctg cggctcgcac gcgagcacgc cgccacggtg
    16381 ctgatcgtca cgcacgccgc cgaggtcgcc gcagccgccg accgccggct gcggctcgcc
    16441 gacggacgcc tgcacgccgt cgacggagcc gcctgatgcc ggcgcggctg ctgctcgccg
    16501 cagccctgcg cgaactcgcc cgccagccgc tgcagttcgc actcgccgtg ctcggcatcg
    16561 cactcggcgt cgccgtcgtg ctcgccgtgc gtctggccac ggccagcgca ctcgacggct
    16621 tcgagcgcgc cgccgacgcg ctcggcggcc gggccacgca cgcactgctc gccggccctg
    16681 ccggcgtgcc ggacgacgcc tggcgctggc tgcgcatcga ggccggcgtg cgcagtgccg
    16741 caccgctggt gcagggctgg gtcgagcgca ccgatgcccc cgggcgctgg ctgcagctga
    16801 tcggcgtcga tccctgggcc gaggcaccgc tcgcgcgcgg cggcgcccgg ctcggcggct
    16861 ggccggcgca gctgacgacg cagcccgacg ccgtgctcgc aagcagcgcc ctcgcccgcc
    16921 ggctgcagct cgccgtcggc gagcggctga cgatccgcgc cggcggccgc acgcagtcgc
    16981 tgcgcatcgc cggccgcttc gaggccgacg acgatgccgc tgccgagcgc ctgattgccg
    17041 acatcgccac cgcgcaggcc gtgctcgggc gcgccggccg gctcgatcgc atcgatctgg
    17101 tgctcgatga cgccgccgcc gctgccctcg ccgcccgcct gccgccgccg cttcgccttg
    17161 aaaccgccgc ggcgcgcgcc ggcgaactcg gccggctgac cgccgccttc cgcttcaacc
    17221 tcgacgcgct gagcctgctc gcgctgctgg tcggcgcctt cctgatccgc aacacgctgg
    17281 agttcgccgt cgtgcgccgc cggccgctgc tcgggctgct gcgcgcgctc ggcgtcacgc
    17341 gacgtgagct gctcgggctg atcgcactgg aggccgccgt gctcggcctg ctcggcagcg
    17401 cactcgggct gctgctcggc gccctgctcg cgcgcggcct gatcgggctg gtcgggcgca
    17461 cgctcgccga ggtctacca
//
LOCUS       DAOQIE010000032        17460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1512_length_17460_cov_7.642402, whole genome shotgun sequence.
ACCESSION   DAOQIE010000032 DAOQIE010000000
VERSION     DAOQIE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 17460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17460
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1512_length_17460_cov_7.642402"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK144_03565"
     CDS             <1..591
                     /locus_tag="PK144_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNF64834.1"
                     /translation="EIDAHLAEADIVIGSTASPLPLLMRPAVERALKSRRHRPVLMID
                     IAVPRDIDPAVGTLEDVYLYTVDDLQEIIQENLRSRQAAALQAEEIIDAQVTHFMAWL
                     RAQQANRSIHALRRQSEAVRDELLAKAAARLAAGHDPQAVLEQFAHLLTNKLIHAPSV
                     GLREAGARGDLDTVALIGALYRLDAGPSSSDQSSAA"
     gene            588..1667
                     /gene="prfA"
                     /locus_tag="PK144_03570"
     CDS             588..1667
                     /gene="prfA"
                     /locus_tag="PK144_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNF64835.1"
                     /translation="MKPSIRTRLEALAERHEELARLLAEPEVIADAERFRRHSQEYAQ
                     LEPVATAWRAWGEQQQQLEAARALALDADPELRALAAEEVAQLEAAAGAGERELALAL
                     LPPDPHDSANLFLEIRAGTGGDEAAIFAGDLLRMYLRYAERRGWQVEILSESPGEHGG
                     CKEVIARVAGRGAYARLKFESGTHRVQRVPETEAQGRIHTSAATVAILPELDEVEVRL
                     DPKDLRIDTFRASGAGGQHINKTDSAIRITHLPSGTVVECQDERSQHKNRARALSLLA
                     ARLLDAETSRQQSAQAASRRLQVGSGDRSERIRTYNFPQGRITDHRINLTLYRLGDVI
                     DGALDELLEPLRREDEAERLATLAD"
     gene            1670..2530
                     /gene="prmC"
                     /locus_tag="PK144_03575"
     CDS             1670..2530
                     /gene="prmC"
                     /locus_tag="PK144_03575"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019337038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNF64836.1"
                     /translation="MTAPDAPATLSGLLATALTGLTAAGSDSPRADAEALLAHVLARP
                     RSFLRAWPEAPVAPEAAARFLTLLERRMHGEPVAYLSGRRGFWTLELEVTPATLIPRP
                     ETELLVELALARLPDGRDLRVADLGTGSGAIALALAAERPRIEVTATDASPDALTIAR
                     ANAARLGLDNLRFAAGDWCAALEGRYALIASNPPYLAEDDPHLGRGDLRFEPRRALAA
                     GADGLADLRRIVDQAAAHLEADGWLLLEHGHDQGEAVPALLAAAGYRAVACVPDLGGR
                     PRVSLAQAPA"
     gene            2527..3300
                     /gene="moeB"
                     /locus_tag="PK144_03580"
     CDS             2527..3300
                     /gene="moeB"
                     /locus_tag="PK144_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNF64837.1"
                     /translation="MSALPELDDAQLARHARHVLLAEIGYEGQQRIRAGHALILGLGG
                     LGSPVALYLAAAGTGTLTLVDDDRVELSNLQRQIAHADADRGRLKAESAAGRCRALNP
                     ALRVQTVCTRPDAAALAALVAAADVVLDCSDNFPTRHALNLACRAARRPLVSGAAIRW
                     SGQLAVFDAREPDAPCYRCLYPEADEAAAETCTANGVIAPLVGVIGALQASEALQLLA
                     GRPAALAGRLLRYDALGARLRSARLPRDPDCPVCGRRAG"
     gene            complement(3457..6273)
                     /gene="prsT"
                     /locus_tag="PK144_03585"
     CDS             complement(3457..6273)
                     /gene="prsT"
                     /locus_tag="PK144_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNF64838.1"
                     /translation="MTNTRTNTRSGARTARRQALALGLTLALTVASPGVQAGPAADKY
                     IERARTALQKGDVNAGVIELKNALQEEPDNAAARLLLGQTYLRGGDLAAAEKELARTR
                     DLGLPRAQWVVDYARTLMLLGQADRVLKEFSPLDDDPPETRASLLSLQALALMAQKQL
                     DDAKTRLNEALAAKADNVEALLAMTRLEGAEGKFAEASERVNQVLAVQPDNVEALLLL
                     AELERLQKRYPEAIAAFDKVLARNPKELRALVGRGETRLTQGDADGALADGEAARRLR
                     SNLPAANFLRGRALLAKKNLPAAQEALLEVLRFVPDHLPSQLLLGAIAFQNNNLEQAD
                     AYLSRYVAAVPNQLPARKLLAGTAMKLKQPKRAIDLLAPVAAAGSGDAQLLALLGSAY
                     LQAGDHDKAAALLQKASELAPNASPIRAQHALSLLASGDTDKAIDELETASELNQGLV
                     QADVMLVLTLLQTRDFERAAKTAQALAEKEPKSAMPLNLLGIAEMGQNDLVSARKAFE
                     QALAVDPKFTVAELNLAQIDLRENKPEAAEARYQAVVARDPGNVQALLALAERAERAQ
                     QPVQAVQWLEQAWEKNPDSLQVGLLLAQRYLQRGDRLKASGVVRQLETAQPNNPVVQR
                     MLGAVLFASEDIGGARAAYRKFADAQPNNPEGWFLIASTYLREKDDTGAEQALQRALN
                     IDPKYAPALAGRIELALRGRREAEFRKQVEALRNAFPDSPLPQKYEGDWELQANRPQN
                     AALSYAAAYQRSPSGPLVLQLASARLRAADQAGALAALKDWVDRSPEDYITRLQYAIE
                     LQNQKQADPAIEQYRKVVGTQPRSVIALNNLAWLLSERKDPEAVGFAEKARELAPDSP
                     EVADTLGWVLAQAGKVDRGLQILQQAAVQAPHLGDIRYHLAAVLNQAGRKDEAIRELE
                     RLLGEGRSFEQQEAAKALLAQLKK"
     gene            complement(6355..7710)
                     /gene="prsR"
                     /locus_tag="PK144_03590"
     CDS             complement(6355..7710)
                     /gene="prsR"
                     /locus_tag="PK144_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HNF64839.1"
                     /translation="MRELNRKLLIVEDDPGLQSQLRWCFADYEVLTADSRDSAIAQLR
                     RHEPPIVTLDLGLPPDPANATEGLATLQEILQLLPSTKVIVVTGNDDRDNALQAVAAG
                     AYDFYQKPVDGDVLKLIVDRAHALYALENENRELAARSQSSKLEGLIAGSASMLKVCR
                     TVEKIAPTDATSLLLGDSGTGKELIARALHDLSNRADKRFVAINCAAIPETLLESELF
                     GYEKGAFTGAAKQTRGKIEYADGGTFFLDEVGDLPMSLQAKLLRFLQQRVIERVGGRE
                     EIPVDVRVVCATHRELPALIRAGSFREDLYYRISEITLNIPPLRERDGDTVLIARAFL
                     ERYSQKLGKSFKGYTREALTALENYAWPGNVRELENKVKRAVIMAEGSQITADDLELE
                     EGGEPALPLNLRRVREQAERQALTRAIAYVDGNLSQAADILGITRPTLYALLNKYNMK
                     T"
     gene            complement(7698..9833)
                     /gene="prsK"
                     /locus_tag="PK144_03595"
     CDS             complement(7698..9833)
                     /gene="prsK"
                     /locus_tag="PK144_03595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HNF64840.1"
                     /translation="MLIFSDYGQPGLGELGYGIATLMYLVFAAALATQWRGRLRGAVL
                     VAAVAITAGWTLSAALVAAGVRAALPFYLALETLHAAAWLYFLIVLLRPLILAQQHRH
                     VALAIARPATGALAAALLLVDLLPAVADWLPESLLRDLRIFGHLAMAVGGLVLIEQLF
                     RNTRPEQRWGTKFLYLGVGALFAYDFFLYADTLLFRRMDPAMWAARGFVDAMAVPLIG
                     IAAMRNPEWSPGLFVSHRMALHTASVLGAGIYLLVMAGVAYYIRLYGGNWGTALQLVF
                     LTGAVILLLMMMFSGQLRAYAKVFLSKHFFHYKYDYREEWLKFIATLSRPEPGLPLRV
                     QSVRAMAELVDSGGGVLWSVNEHGSYSFAAAWNSPEPPVRTLAGGESLVRFLAARHWV
                     VELGEYLSEPELYEGLELPAWLCGLSRAWLIVPLMRGDTLQGCLLLLAPPGERRINWE
                     DRDLLKTAGRQAAAYLALLDTTEALTNAREFEAFNRLSAFVVHDLKNVAAQLGLVVKN
                     AERHRQNPAFIDDALKTVENATGRMQRLLAQLASRTRATGQARSFDVGEALAEAVQIC
                     SGQAPRPQLAGSGEALVISADHDKFVAIVCHLLRNAQEATAADGRIELTVGCEAGTAV
                     IAVSDSGCGMDETFVRESLFKPFRTTKGNAGMGVGVYEAREFAHALGGELNVVSQPGF
                     GTTFTLRLPLAGEGAPGRAGHRETAKCAN"
     gene            complement(9852..11246)
                     /locus_tag="PK144_03600"
     CDS             complement(9852..11246)
                     /locus_tag="PK144_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNF64841.1"
                     /translation="MIRLFRHYVPLPLLLLALVEMLVFFGSIPFGKFVRSGIEGWAFD
                     PIGLELKASLFAIVMVSVMTAMGLYERHFWDGKADMMLRVAVGFLLGLFTITLIYYFL
                     PDLYMGRGEFGIAFAASFVCVVVARYAFFRLSDHEALKRRVLVLGTGYKAHQVEQLAS
                     GSHGRGFALIGFVRMNEADDIIVDAARVLPAGTLLRRYTEHERIDEIVVAMDDRRRGF
                     PVDDILDCKINGVTVSDLATFFERESGKIRLDALHPSSMIFSDGFHQAALKSFSKRCF
                     DIIASLLLLGLTWPVMLATAIAIWWESNRNGPIFYKQARVGRNDRVFDVLKFRSMRTD
                     AEQNGIAQWAQKNDSRITRVGAFIRKTRIDELPQLFNVLRGEMSFVGPRPERPQFVSM
                     LEQTIPYYSMRHRVNPGITGWAQICYPYGASEEDAREKLQYDLYYIKNYSLFLDFMVL
                     LQTAHVILWGKGAR"
     gene            complement(11392..12105)
                     /gene="dnaQ"
                     /locus_tag="PK144_03605"
     CDS             complement(11392..12105)
                     /gene="dnaQ"
                     /locus_tag="PK144_03605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNF64842.1"
                     /translation="MRQIVLDTETTGLEPAQGHRIIEIGCVELIGRRPTGRSFHRYLN
                     PERALDPGAVEVHGLTEAFLADKPRFAEVADELLEFIRGAELVIHNAAFDTGFLDAEL
                     ARLKPAPGRIADFCPVLDTLKLARELHPGARASLDALCRRYGIDNSQRELHGALLDAR
                     ILADVYLAMTGGQVAMALDGADAPASAAARSAEPEAVVRIAAGRPALRVIRATAADLE
                     ADERLQALFGHRFEGGPAT"
     gene            complement(12161..12637)
                     /gene="rnhA"
                     /locus_tag="PK144_03610"
     CDS             complement(12161..12637)
                     /gene="rnhA"
                     /locus_tag="PK144_03610"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNF64843.1"
                     /translation="MNAPTTADADLVVIHTDGACSGNPGPGGWAAILNWRGRSRELSG
                     ASAATTNNIMELTAAIMALEALKRPSRVRLHTDSRYVMDGITRWIHGWKKNGWKTSAR
                     EPVKNAGLWQRLDAACAPHTIEWCWVRGHAGDPGNERADQLARAAIGTLRSPPPAG"
     gene            complement(12634..13389)
                     /locus_tag="PK144_03615"
     CDS             complement(12634..13389)
                     /locus_tag="PK144_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64844.1"
                     /translation="MILIFHKKNSLRIAGYAGLDAWYGSGFGQRLAGAEHAQLAALLP
                     LLFGWHAVTLGAPGGHDWLAASRIGHKLRIVPVPALAGEVCADPLALPLRPDSLDLVV
                     VPHLHEFVARPEELFDGIGTALIPDGHVVVLGFNPLLDGGIWRLLRVREARLPAGARL
                     YGSAVVRGWLRRAGFETDAVRFHCRWPWDGALRLRGWPAALGACGYLIVGRKRVSTLT
                     PVRPRWRPRRRLVPAGAVGDASRVGQCCEDRIR"
     gene            13589..15016
                     /locus_tag="PK144_03620"
     CDS             13589..15016
                     /locus_tag="PK144_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNF64845.1"
                     /translation="MSSIRTGAAALAALLLAACAQAPQRPAPADFAATTDDGDLWQRL
                     RGQLSFADTSHPDVQRQLQSLRPRSIASVADNASNYLYFVLDATQSRGMPAEVALVPW
                     FESGYRPNAVSPGRAAGLWQLMPDTATHLGLSLGRGVDERKDVKASTEAALDYLARLN
                     RNFDGDWLLTLAAYNAGPKTVQNAIERNRRAGRPTAFWDLDLPRITQEYVPRILALSQ
                     MIASPERFGLNLPDAGENSALTHVDLGPGVNPMLAAAAAGLSREELLRYNARLRGRDD
                     TGSTELLLPKARAEALARVRPRIEGTKLAVWEGYRLAPGDTIEAIAERFDTSPAALRE
                     VNREAGATLSFTAGREVLVPARGPVRLTMAAAPAQRPKPQTGRLARAKKPGKPEHRPA
                     EAGDGLFAGDVPLRERIRRLLTINLTVRPGDSLAVLSGQYSVAIEDLRRWNDLPASTP
                     AGEPLEAGRTVVVYVDAIADDIPRG"
     gene            complement(15064..16728)
                     /locus_tag="PK144_03625"
     CDS             complement(15064..16728)
                     /locus_tag="PK144_03625"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNF64846.1"
                     /translation="MAEVEVLPPPREVLLAIEELSVGFGEPGAAAVRAVDGVSFTIAP
                     GERLALVGESGSGKSVTALAALGLLDAAQAQVSGSVRFAGQELRALPEAALRRLRGRE
                     IGLIFQEPMNALNPLHPVGRQIAEGLRLHFGLDAGAAWQQAVGLLERTGIPEPQQRAL
                     AYPHMLSGGQRQRAMIAMALACRPKLLIADEPTTALDVTLQVQVLDLLRELQAEFGMA
                     ILFISHDLNLVRRFAGRVCVMHRGRIVEQGPVETVFTAPREPYTRTLLASRPQRLIPD
                     GEHPAQSGAEALLSARGVDVRFVTRRDWLGRPRAAVHALVATDLDVHAGETLGIVGES
                     GSGKSTLGLALLRLLPAAGRIVVDGQEWSGLGQRRLRPLRPAFQVVFQDPFGSLSPRM
                     TVEDIVGEGLRIHRPALDRAQRRARIVAALDEVGLPETALWRYPHEFSGGQRQRIAIA
                     RALVLEPKLLLLDEPTSALDATVQKQVLALLRTLQQRHGMSYLFISHDLAVIRALAHR
                     VLVLRGGQVVESGETAALFSAPQHPYTAALLEAALLPGAGAPAPAG"
     gene            complement(16721..>17460)
                     /locus_tag="PK144_03630"
     CDS             complement(16721..>17460)
                     /locus_tag="PK144_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF64847.1"
                     /translation="PPNPYRYDTIRLDGQRANPAPPSAQHLLGTDDRGRDVLARLIYG
                     FRLSVLFALALTVVGTALGVLAGALQGYLGGRTDLLGQRLIEIWSSLPELYLLIIFAS
                     LFNPSVWLLLALLAAFGWMGLADYVRAEFLRGRNLDYVRAARALGAGNRTIMFRHLLP
                     NSLTPVLTFLPFRISGAIVALTSLDFLGLGVPPETPSLGELLAQGKENLSAWWLSAGT
                     FVVLVGTLLLLIFIGEALREAADTRRG"
BASE COUNT         2568 a         6294 c         6140 g         2458 t
ORIGIN      
        1 gagatcgacg cgcatctggc cgaggccgac atcgtcatcg gctcgaccgc gagcccgctg
       61 ccgctgctga tgcgccctgc cgtcgagcgc gcgctgaaga gccgccgcca ccgcccggtg
      121 ctgatgatcg acatcgccgt gccgcgcgac atcgacccgg cggtcggcac gctggaggat
      181 gtctacctgt acacggtcga tgacctgcag gagatcatcc aggagaacct gcgctcgcgg
      241 caggccgcgg cgctgcaggc cgaggagatc atcgacgcgc aggtcacgca cttcatggcc
      301 tggctgcgcg cgcagcaggc caaccgctcg atccacgcgc tgcgccgcca gtccgaggcg
      361 gtgcgcgacg agctgctcgc gaaggccgcg gcgcggcttg ccgccggcca cgacccgcag
      421 gccgtgctcg aacagttcgc acacctgctg accaacaagc tcatccacgc gccgagcgtc
      481 ggcctgcgcg aggccggcgc acgcggcgac ctcgacaccg tggcgctgat cggcgcgctg
      541 taccggctcg atgccgggcc ttcgtcgtcc gatcagtcct ccgccgcatg aagccgtcga
      601 tccgcacccg tctcgaagcc ctggccgagc gccacgagga actcgcgcgc ctgctcgccg
      661 agcccgaagt catcgccgat gccgagcgct tccgccgcca ttcgcaggaa tacgcgcagc
      721 tcgaaccggt ggccacggcg tggcgggcct ggggcgaaca gcagcagcag ctcgaagcgg
      781 cgcgcgcgct ggcgctcgat gccgatccgg agctgcgcgc gctcgcggcc gaggaggtcg
      841 cgcagctcga agccgcggcc ggggccggcg agcgggaact ggcgctggcg ctgctgccgc
      901 cggacccgca cgacagcgcc aacctgttcc tcgaaatccg cgccggcacc ggcggcgacg
      961 aggcggcgat cttcgccggc gacctgctgc gcatgtacct gcgctacgcc gagcggcgcg
     1021 gctggcaggt cgagatcctg tcggaatccc cgggcgagca cggcggctgc aaggaagtca
     1081 tcgcccgcgt cgccgggcgc ggcgcctacg cgcgcctgaa gttcgaatcc ggcacgcacc
     1141 gcgtgcagcg cgtgcccgag accgaggcgc agggccgcat ccacacctcg gcggcgacgg
     1201 tcgcgatcct gcccgagctc gacgaggtcg aggtgcgcct cgatccgaag gacctgcgca
     1261 tcgacacctt ccgcgcctcg ggcgccggcg gccagcacat caacaagacc gattcggcga
     1321 tccgcatcac gcacctgccg agcggcacgg tcgtcgaatg ccaggacgaa cgctcgcagc
     1381 acaagaaccg cgcgcgcgcg ctgagcctgc tcgccgcgcg cctgctcgac gccgagacca
     1441 gccgccagca gagcgcgcag gccgcgagcc gccggctgca ggtcggctcg ggcgatcgct
     1501 ccgagcgcat ccgcacctac aacttcccgc agggccgcat caccgatcac cgcatcaacc
     1561 tgacgctgta ccggctcggc gatgtcatcg acggcgcgct cgacgaactg ctcgaaccgc
     1621 tgcgccgcga ggacgaggcc gagcggctgg cgacgctggc cgactgatga tgacggctcc
     1681 cgacgccccg gcgacgctgt ccgggctgct ggcgacggcg ctcaccggcc tcaccgccgc
     1741 cggcagcgac tcgccccgcg ccgacgccga ggccctgctc gcgcacgtgc tcgcccgccc
     1801 gcgcagcttc ctgcgcgcgt ggccggaagc accggtcgcg cccgaagcgg ctgcacgctt
     1861 cctgacgctg ctcgaacgcc ggatgcacgg cgagccggtc gcctacctga gcgggcggcg
     1921 cggcttctgg acgctcgaac tcgaagtcac gccggcgacg ctgatcccgc gccccgagac
     1981 cgagctgctg gtcgagctgg cgctggcgcg gctgccggac ggccgggact tgcgcgtcgc
     2041 cgacctcggc accggcagcg gcgcgatcgc gctggcgctc gccgccgaac ggccgcggat
     2101 cgaggtcacg gcgaccgacg ccagccccga tgccctcaca atcgcccgcg ccaacgccgc
     2161 gcggctcggc ctcgacaacc tgcgcttcgc ggccggcgac tggtgcgcag ccctcgaagg
     2221 ccgttacgcg ctgatcgcgt cgaacccgcc gtatctcgcc gaggacgatc cgcacctcgg
     2281 tcgcggcgac ctgcgcttcg agccgcgccg cgcactcgcc gccggcgcgg acggcctcgc
     2341 cgatctgcgc cgcatcgtcg atcaggccgc cgcgcacctc gaagccgatg gctggctgct
     2401 gctcgaacac ggtcacgatc agggcgaggc ggtgccggcg ctgctcgcgg cagccggcta
     2461 ccgcgcagtg gcctgcgtgc ccgatctcgg cggccggccg cgcgtcagcc tcgcccaggc
     2521 cccggcatga gcgccctgcc cgaactcgac gacgcgcaac tcgcgcgcca cgcccgccac
     2581 gtgctgctcg cggagatcgg ctacgagggt cagcagcgca tccgggccgg gcacgcgctg
     2641 atcctcggcc tcggcggcct ggggtcgccg gtggcgctgt atctggccgc ggccggcacc
     2701 ggcacgctga cgctggtcga tgacgaccgg gtggagctga gcaacctgca gcgccagatc
     2761 gcgcacgccg atgccgatcg cggccgcctg aaggccgagt cggccgccgg gcgctgccgc
     2821 gcgctgaacc cggcgctgcg ggtgcagacc gtctgcaccc gtccggatgc tgcggcgctc
     2881 gccgcgctgg tggcggccgc cgatgtcgtg ctcgactgca gcgacaactt cccgacccgc
     2941 cacgcgctga atctcgcctg ccgcgccgca cgcaggccgc tggtcagcgg cgcggcgatc
     3001 cgctggagcg ggcagctcgc ggtgttcgat gcgcgcgagc ccgacgcccc ctgctaccgc
     3061 tgcctgtacc cggaggccga cgaggccgcc gccgagacct gcacggcgaa cggcgtcatc
     3121 gcgccgctgg tcggcgtgat cggggcgctg caggcgagcg aggcgctgca actgctcgcc
     3181 gggcggccgg cggcgctcgc cgggaggctg ctgcgctacg acgcgctggg cgcacggctg
     3241 cgcagcgccc gcctgccccg cgatccggac tgcccggtgt gcgggcggcg agcgggctga
     3301 taccggtcac ccgtgggtac agggtttttc cacccctctc ccgcaagaag tgcgaaaagc
     3361 gaaacgctcc ggtgtgatcg gcatgagccc cggaaacgac acggccggcg gaatcgccgg
     3421 ccgtgtgcct gctgacgggc gggacgtttc agatcatcac ttcttcagct gcgccagcag
     3481 cgccttcgcg gcttcctgct gctcgaagct gcgcccctcg ccgagcaggc gttcgagttc
     3541 gcggatggct tcatccttgc gcccggcctg gttcagcacc gcggcgaggt gatagcggat
     3601 gtcgccgagg tgcggggcct gcaccgccgc ctgctgcagg atctgcaggc cgcgatcgac
     3661 cttgccggcc tgcgccagca cccagccgag cgtgtcggcg acctcggggc tgtccggggc
     3721 gagttcgcgc gccttctcgg cgaagcccac ggcttcgggg tccttgcgtt cgctgagcag
     3781 ccaggcgagg ttgttcagcg cgatgacgct gcgcggctgc gtcccgacga ccttgcggta
     3841 ctgctcgatc gccggatcgg cctgcttctg gttctgcagc tcgatcgcgt actgcaggcg
     3901 cgtgatgtag tcctccggcg agcgatcgac ccagtccttc agcgccgcca gcgcgccggc
     3961 ctgatcggcg gcgcgcagcc gcgccgaggc cagctgcagc accagcggtc cgctcggcga
     4021 acgctggtag gcggcggcat acgacagcgc cgcgttctgc gggcggttgg cctgcagctc
     4081 ccagtcgccc tcgtacttct gcggcagcgg cgaatccggg aaggcgttgc gcaacgcctc
     4141 gacctgcttg cggaactcgg cttcgcgccg gccgcgcagc gccagttcga tgcgcccggc
     4201 cagcgccggg gcgtacttcg gatcgatgtt gagcgcccgc tgcagcgcct gttcggcgcc
     4261 ggtgtcgtcc ttctcgcgca gataggtgct cgcgatcagg aaccagcctt ccggattgtt
     4321 cggctgcgcg tcggcgaact tgcggtaggc ggcacgcgcg ccgccgatgt cctcgctcgc
     4381 gaacagcacg gcaccgagca tgcgctgcac caccgggttg ttcggctgcg cggtttcgag
     4441 ctggcgcacg acgccgctcg ccttcagccg gtcgccgcgc tgcaggtagc gctgcgcgag
     4501 cagcaggccg acctgcagcg agtccgggtt cttctcccag gcctgttcga gccattgcac
     4561 ggcctgcacc ggctgctggg cgcgctcggc gcgctcggcc agcgccagca gcgcctgcac
     4621 attgccggga tcgcgggcga cgacggcctg gtagcgcgcc tcggcggcct cgggcttgtt
     4681 ctcgcgcagg tcgatctgcg cgaggttcag ctccgcgacc gtgaacttcg gatcgacggc
     4741 gagcgcctgc tcgaaggcct tgcgcgcgga cacgaggtcg ttctgcccca tctcggcgat
     4801 gccgagcagg ttcagcggca tcgccgactt cggctctttt tccgccagcg cctgcgcggt
     4861 tttggccgcg cgttcgaagt cacgcgtctg cagcagcgtc agcaccagca tcacgtcggc
     4921 ctgcacgagg ccctggttca gctcgctggc ggtttccagc tcgtcgatgg ccttgtcggt
     4981 gtcgccggag gcgagcagcg acagcgcgtg ctgggcccgg atcggcgagg cgttcggcgc
     5041 gagttccgag gccttctgca gcagggctgc cgccttgtcg tggtcaccgg cctgcaggta
     5101 ggcgctgccg agcagcgcga gcagctgggc atcgccgctg ccggccgccg ccaccggcgc
     5161 cagcaggtcg atcgcgcgct tcggctgctt gagcttcatc gccgtgccgg cgagcagctt
     5221 gcgggccggc aactggttcg gcacggccgc cacgtagcgg ctcaggtagg cgtcggcctg
     5281 ttcgaggttg ttgttctgga aggcgatcgc gccgagcagc agctggctcg gcaggtgatc
     5341 gggcacgaag cgcagcactt cgagcagcgc ctcctgcgcc gccggcaggt tcttcttcgc
     5401 gagcagcgcg cgcccgcgca ggaagttggc ggccggcagg ttgctgcgca gccggcgggc
     5461 ggcctcgcca tcggcgagtg cgccgtcggc atcgccctgg gtgagccggg tttcaccgcg
     5521 cccgaccagc gcgcgcagct ccttcggatt gcgcgccagc accttgtcga aggccgcgat
     5581 cgcttcggga tagcgcttct gcaggcgttc gagctcggca agcagcagca gcgcctcgac
     5641 gttgtcgggc tggacggcga gcacctggtt cacgcgctcg ctggcttcgg caaacttgcc
     5701 ctcggcgcct tcgaggcggg tcatcgccag cagcgcctcg acgttgtcgg ccttggccgc
     5761 cagcgcctcg ttgaggcggg tcttggcatc gtcgagctgc ttctgcgcca tcagcgccag
     5821 cgcctgcagc gacagcagcg aggcgcgggt ttccggcgga tcgtcgtcga gcggcgaaaa
     5881 ctccttcagc acgcgatccg cctggccgag cagcatcagc gtgcgcgcgt aatcgaccac
     5941 ccactgcgcg cgcggcaggc cgaggtcgcg ggtgcgggcg agttccttct cggccgcggc
     6001 gaggtcaccg ccgcgcagat aggtctggcc gagcagcagg cgggcggccg cgttgtcggg
     6061 ttcttcctgc agcgcgttct tcagctcgat gacgccggcg ttgacatcgc ccttctgcag
     6121 ggccgtgcgg gcgcgctcga tgtacttgtc ggccgccggg ccagcctgca cgccggggct
     6181 ggcgaccgtc agtgccagcg tcagtccgag cgccagcgcc tgcctgcgcg ccgtgcgcgc
     6241 accgcttcgc gtattcgtcc gcgtattcgt caccgtggct gtcctgtgtt gcgaagtcct
     6301 gcagagtccc gcgcggtgct gcgcatgaca cggagccggc cgcaagcgcg gacctcaggt
     6361 cttcatgttg tatttgttga gcagcgcgta gagggtcggc cgggtgatgc cgaggatatc
     6421 tgcggcctgc gacagattgc catcaacgta ggcgatcgcc cgcgtcagcg cctgacgctc
     6481 ggcctgctcg cgcacgcggc gcaggttcag cggcagcgcc ggttcgccgc cctcctccag
     6541 ttcgaggtcg tcggcggtga tctggctgcc ttcggccatg atcacggcgc gcttgacctt
     6601 gttctccagc tcgcggacgt tgccgggcca ggcgtagttc tccagcgccg tcagcgcctc
     6661 gcgcgtatag cccttgaagc tcttgccgag cttctggctg tagcgttcga ggaaggcgcg
     6721 cgcgatcagc accgtgtcgc cgtcgcgctc gcgcagcggc gggatgttca gcgtgatctc
     6781 gctgatgcgg taatacaggt cctcgcggaa gctgccggcc cggatcagcg ccggcagctc
     6841 gcggtgcgtg gcgcagacca cgcgcacgtc gaccgggatt tcctcgcgac cgccgacgcg
     6901 ctcgatcacg cgctgctgca ggaagcgcag cagcttcgcc tgcagcgaca tcggcaggtc
     6961 gccgacctcg tcgaggaaga acgtaccgcc atcggcgtat tcgatcttgc cgcgggtctg
     7021 cttggcggcg ccggtgaacg cgcccttctc gtagccgaac agctcgcttt cgagcagcgt
     7081 ttcggggatc gccgcgcagt tgatcgcgac gaagcgcttg tcggcgcggt tcgacagatc
     7141 atgcagcgcg cgggcgatga gctccttgcc ggtgccgctg tcgccgagca gcagcgaggt
     7201 cgcgtcggtc ggggcgatct tctcgaccgt gcggcagacc ttcagcatcg aggcgctgcc
     7261 ggcgatcagg ccttcgagct tcgaggactg gctgcgggcg gccagttcgc ggttctcgtt
     7321 ctcgagcgcg tacagcgcgt gggcacgatc gacgatcagc ttcagcacgt cgccgtcgac
     7381 cggcttctga tagaagtcgt aggcaccggc ggccaccgcc tgcagtgcgt tgtcgcgatc
     7441 atcgttgccg gtgacgacga tgaccttggt cgagggcagc agctgcagga tctcctgcag
     7501 cgtcgccagc ccctcggtgg cgttggccgg gtccggcggc aggccgagat cgagcgtgac
     7561 gatcggcggt tcgtgccggc gcagctgcgc gatcgccgaa tcgcgactgt cggcagtcag
     7621 cacctcgtag tcggcgaagc accagcgcaa ctggctctgc aggcccggat cgtcctcgac
     7681 gatcagcagt ttacgattca gttcgcgcac ttggcggtct cccggtgtcc ggcgcgcccg
     7741 ggcgcgccct cgcctgccag cggcaggcgc aaggtgaagg tggtgccgaa accgggctgg
     7801 ctgacgacgt tgagctcacc gccgagcgca tgcgcgaact cgcgggcctc atagacgccg
     7861 acgcccatgc cggcattgcc cttggtcgtg cggaacggct tgaacagcga ttcgcgcacg
     7921 aaggtttcgt ccatgccgca gccgctgtcg ctgaccgcga tcacggccgt gccggcctcg
     7981 cagccgacgg tcagttcgat gcggccgtcg gccgcggtcg cttcctgcgc attgcgcagc
     8041 agatggcaga cgatcgcgac gaacttgtcg tgatcggcgc tgatcaccag cgcctcgcca
     8101 ctcccggcga gctgcgggcg cggcgcctgc ccgctgcaga tctgcacggc ctcggcgagc
     8161 gcctcgccga catcgaagct gcgggcctgg ccggtcgcgc gcgtgcggct cgcgagctgc
     8221 gcgagcaggc gctgcatgcg gccggtggcg ttctcgacgg tcttcagcgc atcgtcgatg
     8281 aaggccgggt tctggcggtg gcgctcggcg ttcttgacca cgaggccgag ctgcgcggcg
     8341 acgttcttca ggtcgtggac cacgaaggcc gacagccggt tgaaggcttc gaactcgcgg
     8401 gcattggtca gggcctcggt ggtgtcgagc agcgccagat aggccgccgc ctgccggccg
     8461 gcggtcttga gcaggtcgcg atcctcccag ttgatgcgcc gctcgccggg cggggccagg
     8521 agcagcaggc agccctgcag cgtgtcgccg cgcatcagcg gcacgatcag ccaggcgcgc
     8581 gacaggccgc agagccaggc cggcagctcc agaccttcgt acagctccgg ttcggacagg
     8641 tactcgccga gttcgacgac ccagtgccgg gcggccagga agcgcaccag cgattcaccg
     8701 ccggcgagcg tgcgcaccgg cggttccggc gaattccagg ccgcggcgaa ggaatagctg
     8761 ccgtgctcgt tgacgctcca gagcacgccg ccgccgctgt cgacgagttc ggccatcgcg
     8821 cgcaccgact gcacgcgcag cggcagcccg ggctcgggcc gcgacagcgt ggcgatgaac
     8881 ttcagccatt cctcgcgata gtcgtacttg tagtgaaaga agtgcttgct caggaacacc
     8941 ttggcgtagg cacgcagctg ccccgagaac atcatcatca gcagcaggat cacggcgccg
     9001 gtcaggaaca ccagctgcag cgccgtgccc cagttgccgc cgtacagccg gatgtaatac
     9061 gcgaccccgg ccatgaccag caggtagatg ccggccccga gcaccgaggc cgtgtgcagc
     9121 gccatgcggt gcgacacgaa caggcccggc gaccactcgg ggttgcgcat cgccgcgatg
     9181 ccgatcagcg gcacggccat cgcatcgacg aagccgcgcg ccgcccacat cgccgggtcc
     9241 atgcgccgga acagcagcgt gtcggcgtac aggaagaagt cgtacgcgaa cagcgccccg
     9301 acgccgaggt acaggaattt ggtgccccag cgctgctcgg gccgggtgtt gcggaacagc
     9361 tgctcgatca gcaccagtcc gccgaccgcc atcgccagat gcccgaagat gcgcaggtcg
     9421 cgcagcagcg attccggcag ccagtccgcg accgccggca gcaggtcgac cagcagcagc
     9481 gccgcggcca gcgcgccggt cgccggtcgc gcgatcgcga gcgccacatg gcgatgctgc
     9541 tgggcgagga tcagcggccg cagcagcacg atcaggaagt acagccacgc cgccgcgtgc
     9601 agggtttcga gggcgagata gaacggcagc gccgcgcgca cgccggcggc gaccagcgcc
     9661 gccgacagcg tccagccggc ggtgatcgcg acggccgcaa ccagcaccgc cccgcgcagc
     9721 cggccgcgcc actgcgtggc cagcgccgcg gcaaacacca ggtacatcag cgtcgcaatg
     9781 ccgtagccga gttcgccgag cccgggctgg ccgtagtcac tgaagatcag catgtgcccg
     9841 cctccgccgg atcagcgggc ccccttgccc cacaggatca cgtgggccgt ctgcagcagc
     9901 accatgaaat cgaggaacag gctgtagttc ttgatgtagt agaggtcgta ctgcagcttc
     9961 tcgcgggcat cctcctcgga ggccccgtac ggatagcaga tctgcgccca gccggtgatg
    10021 ccggggttca cgcggtggcg catcgagtag tacgggatcg tctgctccag catcgacacg
    10081 aactgcggac gctcgggccg cgggccgacg aagctcatct cgccgcgcag cacgttgaac
    10141 agctgcggca gctcgtcgat gcgggtcttg cggatgaagg caccgacgcg ggtgatgcgc
    10201 gagtcgttct tctgcgccca ctgcgcgatg ccgttctgct cggcatcggt gcgcatgctg
    10261 cggaacttca gcacgtcgaa cacgcggtcg ttgcgaccga cccgggcctg cttgtagaag
    10321 atcgggccgt tgcggttcga ttcccaccag atcgcgatgg ccgtggccag catcaccggc
    10381 caggtcaggc cgagcagcag caggctggcg atgatgtcga agcagcgctt gctgaaggac
    10441 ttcagcgccg cctgatggaa gccgtccgag aagatcatcg aactcggatg cagcgcgtcg
    10501 aggcggatct tgccgctctc gcgctcgaag aaggtcgcca gatcgctgac ggtgacgccg
    10561 ttgatcttgc agtcgaggat gtcatcgacc gggaagccgc ggcggcggtc gtccatggcc
    10621 acgacgatct cgtcgatgcg ctcgtgctcg gtgtagcggc gcagcagcgt gccggccggc
    10681 agcacgcgcg cggcgtcgac gatgatgtcg tcggcctcgt tcatgcgcac gaagccgatc
    10741 agcgcgaaac cgcggccgtg gctgcccgag gcgagctgct cgacctggtg ggccttgtag
    10801 ccggtgccga gcaccagcac gcggcgcttg agcgcctcgt ggtccgacag ccggaagaac
    10861 gcatagcggg cgacgaccac gcagacgaag gaagccgcga acgcgatgcc gaactcgccg
    10921 cggcccatgt agagatcggg caggaagtaa tagatcagcg tgatcgtgaa caggccgagc
    10981 aggaagccga ccgcgacgcg cagcatcatg tcggccttgc cgtcccagaa gtggcgctcg
    11041 tacagcccca tggccgtcat gaccgagacc atgacgatcg cgaacagcga ggccttcagc
    11101 tccagcccga tcggatcgaa ggcccagccc tcgatgccgc tgcgcacgaa cttgccgaac
    11161 gggatgctcc cgaagaagac cagcatttcc acgagggcca gcagcagcag cggcagcggc
    11221 acgtaatgtc tgaacaggcg aatcatccgt catccccagg ggcaagtccg cttcggcgtc
    11281 cggttccggg gcagccggtc ggtcgcgaca acggggccag actctaccat cggcccgccc
    11341 gcccccgtcc gaacgagctg tgtcgccgct gtgtaaagtt gataaccggc ttcaggtcgc
    11401 cggaccgccc tcgaaacggt ggccgaacag cgcctgcagc cgctcgtcgg cttcgaggtc
    11461 cgcggccgtg gcccggatca cgcgcagcgc cggccgcccg gccgcgatgc gcacgacggc
    11521 ctcgggctcg gcgctgcggg ccgcggcgct ggccggcgca tcggcaccgt cgagcgccat
    11581 cgccacctga ccgccggtca tcgccagata gacatcggcg aggatgcgcg cgtcgagcag
    11641 cgcgccgtgc agctcgcgct gggagttgtc gatgccgtag cgccggcaca gcgcatcgag
    11701 cgaggcgcgc gcgccgggat gcagctcgcg cgcgagcttc agcgtgtcga gcaccgggca
    11761 gaaatcggcg atgcgcccgg gcgcaggctt caggcgtgcc agctcggcat cgaggaagcc
    11821 cgtgtcgaac gcggcgttgt ggatgaccag ctcggcgccg cggatgaact ccagcagttc
    11881 atcggccacc tccgcgaagc ggggcttgtc ggccaggaag gcctcggtca ggccatggac
    11941 ctcgaccgcc ccgggatcga gcgcccgctc cgggttcagg tagcggtgga agctgcgccc
    12001 ggtcggccgg cggccgatca gctcgacgca gccgatctcg atgatgcggt gaccctgggc
    12061 cggttcgagt ccggtggttt cggtgtcgag gacgatctgg cgcacgacgg aggactcgcg
    12121 aggtgggggg aaggagcagg gtttcgacgg tccggacggt tcagcccgcc ggcggcggac
    12181 tgcgcagcgt gccgatcgcg gcgcgggcga gctgatcggc gcgttcgttg ccggggtcgc
    12241 cggcatggcc gcgcacccag caccattcga tcgtgtgcgg tgcgcaggcg gcatcaagcc
    12301 gctgccagag tccggcattc ttgaccggct cgcgggcgct ggtcttccag ccgttcttct
    12361 tccagccgtg aatccagcgc gtgatgccgt ccatcacgta gcggctgtcg gtgtgcaggc
    12421 gcacgcggct cgggcgcttc agcgcctcca gcgccatgat cgccgcggtc agctccatga
    12481 tgttgttggt cgtcgcggca ctggcaccgc tcagctcgcg gctgcgtccg cgccagttca
    12541 ggatcgccgc ccagccaccc ggccccgggt tgccggagca ggcaccgtcg gtatggatca
    12601 cgaccagatc cgcatcggcc gtcgtcggag cgttcaccgg attcgatcct cgcagcactg
    12661 gccgacgcgc gaggcatcgc cgaccgcccc ggccggcacc agccgccggc gcgggcgcca
    12721 gcgcgggcgc accggcgtca gcgtcgagac gcgcttgcgc ccgacgatca ggtaaccgca
    12781 ggcaccgagc gccgccggcc agccccgcag ccgcagcgca ccgtcccagg gccagcggca
    12841 gtggaagcgc acggcgtcgg tctcgaagcc ggcacgccgc agccagccgc gcacgacggc
    12901 actgccatac aggcgcgccc cggccggcag ccgcgcctcg cgcacgcgca gcagccgcca
    12961 gatgccgccg tcgagcagcg gattgaagcc gagcacgacg acgtgtccgt ccgggatcag
    13021 cgccgtgccg atgccgtcga acagttcttc gggcctggca acgaattcgt gcaggtgcgg
    13081 cacgacgacc agatcgaggc tgtcgggccg cagcggcagc gccagcggat cggcgcagac
    13141 ctcgccggcc agggccggca ccggaacgat gcgcagctta tggccgatgc ggctcgccgc
    13201 gagccagtca tgcccgcccg gtgcccccag cgtcaccgcg tgccagccga acagcagcgg
    13261 cagcagcgcg gccagctggg catgctcggc cccggccaga cgctggccga aaccgctgcc
    13321 gtaccaggcg tcgagaccgg cgtaaccggc gatgcgcagg ctgtttttct tgtggaagat
    13381 caggatcatg cacctgctga gcgggccttg ttgcgtgcgc gccgcaggct tgccacgctc
    13441 cgccgccacc gctagattgc cgccgcaaca aagtaacaca cgcagcaaca cggccgacga
    13501 cgcctgtacg gcgaccgcca cgcgcacgga tgcacgggcg gcataacgat ccggggccga
    13561 tacaacccga accgaaggta tcgcaccgat gtcgtcgatc cgtaccgggg ccgccgcgct
    13621 ggcggccctg ctgctcgctg cctgtgccca ggcgccccag cgccccgccc ccgccgattt
    13681 cgccgccacc accgatgacg gtgatctctg gcagcggctg cgcggccagc tgtcctttgc
    13741 cgacacctcg cacccggacg tgcagcgcca gctgcagtcg ctgcgcccgc gcagcatcgc
    13801 ctcggtggcc gacaacgcct cgaactacct gtacttcgtg ctcgatgcca cgcagagccg
    13861 cggcatgccg gccgaggtcg cgctggtgcc gtggttcgag agcggctacc ggccgaacgc
    13921 ggtgtcgccg ggccgcgccg ccggcctctg gcagctgatg cccgataccg ccacgcatct
    13981 cggcctgagc ctcggtcgcg gcgtcgacga gcgcaaggac gtcaaggcct cgaccgaggc
    14041 cgcgctcgat tacctggcgc gcctgaaccg caacttcgac ggcgactggc tgctgacgct
    14101 cgccgcctac aacgccggtc cgaagaccgt gcagaacgcc atcgagcgca accgccgcgc
    14161 cggccgcccg accgcgttct gggacctcga cctgccgcgc atcacgcagg agtacgtgcc
    14221 gcgcatcctg gcgctgtcgc agatgatcgc gagccccgag cgcttcggcc tgaacctgcc
    14281 ggatgccggc gagaactcgg cgctgaccca tgtcgacctc ggtcccggcg tcaacccgat
    14341 gctcgccgcc gccgccgccg gcctgagccg cgaggaactg ctgcgctaca acgcccggct
    14401 gcgcggtcgc gacgacaccg gcagcaccga gctgctgctg ccgaaggccc gggccgaggc
    14461 gctggcgcgc gtgcgcccgc gcatcgaggg cacgaagctc gcggtctggg aaggctaccg
    14521 gctggcgccg ggcgacacga tcgaggcaat cgccgagcgc ttcgacacca gccctgcggc
    14581 gctgcgcgag gtcaaccgcg aggccggtgc gacgctgagc ttcacggccg gacgcgaggt
    14641 gctggtgccg gcgcgcggcc cggtgcgcct gaccatggcc gcagccccgg cgcagcggcc
    14701 gaagccccag accgggcggc ttgcgcgcgc gaaaaagccc ggcaagccgg agcaccggcc
    14761 ggcggaagcc ggcgacggcc tgttcgccgg cgatgtaccg ctgcgcgagc gcatccgccg
    14821 gctgctgacg atcaacctga ccgtgcgccc cggcgattcg ctggccgtgc tctcggggca
    14881 gtacagcgtc gcgatcgagg atctgcgccg ctggaacgat ctgccggcct cgacgccggc
    14941 gggcgagccg ctcgaagccg gccgcaccgt cgtcgtctac gtcgacgcga tcgccgacga
    15001 catcccgcgc ggctgaagcc ggccaagagt ttcgcgccgg ccggggggct tcccgtcccg
    15061 gcttcagccg gccggcgccg gtgcaccggc gcccggcagc agcgcggctt cgagcagcgc
    15121 ggccgtgtac gggtgctgcg gcgccgagaa cagcgccgcg gtctcgccgg actccacgac
    15181 ctgaccgccg cgcagcacca gcacgcggtg cgcgagcgcg cggatcaccg ccaggtcgtg
    15241 gctgatgaac aggtagctca tgccgtggcg ctgctgcagc gtgcgcagca gcgccagcac
    15301 ctgcttctgc accgtcgcat cgagtgcgct ggtcggctca tcgagcagca gcagcttcgg
    15361 ctccagcacc agcgcccgcg cgatcgcgat gcgctggcgc tggccgcccg agaactcatg
    15421 cggataacgc cacagcgcgg tttccggcag gccgacctcg tcgagcgcag cgacgatgcg
    15481 ggcacggcgc tgcgcgcgat cgagcgccgg acggtggatg cgcaggccct cgccgacgat
    15541 gtcctccacc gtcatgcgcg gcgacagcga gccgaacggg tcctggaaca cgacctggaa
    15601 ggccgggcgc agcggccgca gccggcgctg gccgagtccg ctccattcct gcccgtcgac
    15661 gacgatgcgc ccggcagccg gcagcagccg cagcagcgcg agcccgagcg tcgacttgcc
    15721 cgaaccgctc tcgccgacga tgccgagcgt ctcgcccgca tgcacgtcga ggtcggtcgc
    15781 aaccagcgca tgcaccgcgg cgcgcggccg gccgagccag tcgcggcgcg tcacgaagcg
    15841 cacatcgaca ccacgcgcgc tgagcagcgc ttcggcgccg ctttgcgccg gatgttcgcc
    15901 gtccgggatc agccgctgcg ggcggctcgc gagcagcgtg cgcgtatacg gctcgcgcgg
    15961 cgccgtgaac accgtctcga ccggtccctg ctcgacgatg cggccacggt gcatgacgca
    16021 gacgcgcccg gcgaagcggc gcaccaggtt caggtcgtgg ctgatgaaca ggatcgccat
    16081 gccgaactcg gcctgcagtt cgcgcagcag gtcgagcacc tgcacctgca gcgtcacgtc
    16141 gagcgccgtg gtcggctcat cggcgatcag cagcttcggc cggcaggcca gcgccatcgc
    16201 gatcatcgcc cgctggcgct ggccgccgga gagcatgtgc gggtaggcaa gcgcccgctg
    16261 ctgcggctcg gggatgccgg tacgttccag cagcccgacc gcctgctgcc aggcggcacc
    16321 ggcatcgaga ccgaagtgca ggcgcaggcc ctcggcgatc tggcggccga ccgggtgcag
    16381 cgggttcagc gcgttcatcg gctcctggaa gatcaggccg atctcccggc cgcgcagccg
    16441 gcgcagcgcc gcctcgggca gggcccgcag ctcctggccg gcgaaacgca cgctgccgct
    16501 cacctgcgcc tgcgcggcat cgagcaggcc gagcgcggcg agcgcggtca ccgacttgcc
    16561 ggagccgctc tcgccgacca gcgccaggcg ctcgccgggc gcgatcgtga agctgacgcc
    16621 atcgaccgca cgcacggccg cggcgcccgg ctcaccgaag ccgacgctca gctcctcgat
    16681 cgcgagcagc acctcccgcg gcggcggcag gacttcaact tcagccacgg cgcgtatccg
    16741 ccgcctcgcg cagcgcctcg ccgatgaaga tcagcagcag cagcgtgccg accagcacga
    16801 cgaaggtgcc ggccgacagc caccaggcgc tcaggttctc cttgccctgc gccagcagct
    16861 cgccgaggct cggggtttcc ggcggcacgc cgaggccgag gaaatcgagg ctggtcagcg
    16921 ccacgatcgc gccgctgatg cgaaacggca ggaaggtcag caccggcgtc agcgagttcg
    16981 gcagcaggtg gcggaacatg atcgtgcggt tgccggcgcc gagcgcgcgc gccgcgcgca
    17041 cgtagtcgag gttgcgcccg cgcaggaact cggcgcgcac gtagtcggca agccccatcc
    17101 agccgaaggc cgcgagcagc gcgagcagca gccagacgct cggattgaac agcgacgcga
    17161 agatgatcag caggtacagc tccggcagcg aggaccagat ctcgatcagc cgctggccga
    17221 gcaggtcggt gcgcccgccg agatagccct gcagcgcgcc ggcgagcacg ccgagtgcgg
    17281 tgccgaccac ggtcagcgcc agtgcgaaca gcaccgacag ccggaagccg tagatcagcc
    17341 gcgccagcac gtcgcggccg cggtcgtcgg taccgagcag gtgctgcgcg gacggcggcg
    17401 ccgggttggc gcgctggccg tcgaggcgga tcgtgtcgta gcggtagggg ttcggcggat
//
LOCUS       DAOQIE010000033        17402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1519_length_17402_cov_7.746239, whole genome shotgun sequence.
ACCESSION   DAOQIE010000033 DAOQIE010000000
VERSION     DAOQIE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 17402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17402
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_17402_cov_7.746239"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2521)
                     /locus_tag="PK144_03635"
     CDS             complement(<1..2521)
                     /locus_tag="PK144_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF64848.1"
                     /translation="MGPDADTPEGRAARVDALDRELAATLDCLDQALLTFDGTLCLRR
                     YSARAARLFGLSDDALGRPASGLDGLATFPGLVRRLETVLTVGGRYADELHSDRAVWL
                     LQITRIDADDPQAGLVCALIETTPLRAAHRALIESEERYALAVEAAQDGLWEWLPAED
                     ALFLSPRLCTLLGCSDAGRPASGAALRERIDPADRPRYDEALAAHLSGRTPAVQIELR
                     MRRDDGTRLWVELHALARRDGAGRAMRVAGSIRDIADRRRTEAALRNSEEALSRTFAH
                     APIGMAVVDLEGRFQRVNRELALITGYEESELLGRNFRAITHPDDLERDLALVEALLT
                     GERESYTLEKRYLRRDGRAVWVLLSVAIVRDTAGRPLHFTAQMVDISARKDIEQALAA
                     AEQRWHYALEGSGLGVWDWNAETDRIFYSPRWKEMLGYADDEIGDTLDEWEQRLHPED
                     REAVHAALKLHYAGGTDYYEAEYRLRCRDGGWKWILDRGQVLERDADGRPLRAFGTHL
                     DIDKRKHAEAALAEALRAASAAAAAKSVFLTNMSHEIRTPLNAVLGLARLAERRAVDP
                     LQRDWLTRLRGAGATLLALVDDILDASRIEAGGLSLERTHFTLDTVLSAVADQHTGAA
                     RAQGLEFTIDAAPGTPARWLGDPLRVQQILGNLVDNAVKFTAQGRVGVEVSVRTREGC
                     AQLCLAVEDTGIGIDPARIEKLFDAYAQGDSSMTRRYGGSGLGLTIARRLAEAMGGTI
                     EVDSSPGRGSRFTACLALEADEPAPAMPLLAGRTLCIQGPPGPVGAQLRRLAAEHGAR
                     LVSDPVAADWLLLEAAAVAAQAPPAGSRTRCAALVWRAAASP"
     gene            complement(2672..4078)
                     /locus_tag="PK144_03640"
     CDS             complement(2672..4078)
                     /locus_tag="PK144_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF64849.1"
                     /translation="MPHRPRRLRLAPASLFGRLLLLLLAGLGAALALSAWLTLTDRGE
                     ALYRVLVTDNAERIANLVELLDGLDEGDRARVVDALDLPPLYIRLDLAWAGNPAEVSP
                     QAAALAGALHAQLGDDRPLAVSLSHGEAAGSGRDEDDGKGRRAPSFFVQVGLADGGTV
                     SFLYVLPRFIGAQPVRLLAALALVSAIVLAVSVLAVRGLTRPLARLAGAARELGTDLE
                     RPPLPETGPVEVRDAAIAFNDMQARLRRQLAERDQLLAAVSHDLKTPLARLRLRLELI
                     GESGVQARALADLADLETTVADTLDFLRAGREEATVPVAIGALAEALADDAADTGAAV
                     EVLGDGGAPLPARPRALRRCLQNLLDNALRYGNSDGEADAPVEIVLHDAPQALCICIR
                     DRGPGLPAAELERVFEPFRRLEHSRSRATGGTGLGLSIARAIARGHGGELTLANRSDG
                     PGLEARLTLPRRPLAGAR"
     gene            complement(4089..4832)
                     /locus_tag="PK144_03645"
     CDS             complement(4089..4832)
                     /locus_tag="PK144_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF64850.1"
                     /translation="MNEHILIVDDDPELRRLLADYLGRNGLRVSEAGDGTAMHAALAA
                     GGIELVILDLMLPGEDGLSLCRSLRARSALPVIMLTARGDDTDRIVGLEMGADDYLPK
                     PFNPRELLARIRSVLRRAQNPGPAANPAAAGGAARGEIAAFRFHDWTLDVAARQLVDP
                     QGTIVALGSAEYRLLKALAEHGGRVLSRDRLLDLTQGREAGPFDRSVDMLVSRIRRRL
                     GDDPKEPAIIRTVRNEGYLLTGPVTREYG"
     gene            4981..5580
                     /locus_tag="PK144_03650"
     CDS             4981..5580
                     /locus_tag="PK144_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNF64851.1"
                     /translation="MSPNFHDRHPNPSPALRRRSALLVAALAATLAMPLAHARPPMDG
                     PDDGPGMHEGRDPARHWARMHERMEQRFEQLEQALKLRPEQKPVWDAFVAARPQPPQR
                     SPEEWRQWREQMQTMGAIERHKAMEQRLEERMQQMKASGVALQKLYDVLDTSQRATLG
                     RFQWWPGPKGMKDGYGPGPARTPAPPPAAPVAPAAPPAQ"
     gene            6092..7840
                     /locus_tag="PK144_03655"
     CDS             6092..7840
                     /locus_tag="PK144_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HNF64852.1"
                     /translation="MNRLALGVSLLLGVASAAVAGVTDQNLLDAGVKDNKSVLTWGID
                     YQGTRYSPLKQINADTIKDLVPAWTFSYGDEKQRGQEAQPLVYNGKIFVTASYSRIYA
                     VDAKTGKKLWVYEHRLPDGILPCCDVINRGAALYGDLVIYSTLDAQLVALNQETGKVV
                     WREKMDNYKDGYSNSAAPIVVKGLVITGVSGGEFGVVGRVDARDALTGKLVWTRPVIE
                     GHMGYTFKDGQKTEAGLTGETGKTWPGDLWQTGGGATWLGGYYDPETDSVLIGTGNPA
                     PWNAHTRPGDNLYSASSLAIDPSTGKIKWHYQTTPNDGWDFDGVNELVSFDLKKDGKT
                     IKAVGKADRNGFFYVLDRTNGKLISATPFVTKQTWATGVDLKTGRPIETGVRPGDPSK
                     SADGKKGETVFSAPSFLGGKNWMPMAYNKDTGLFYVPANEWGMDIWNEPITYKKGAAY
                     LGAGFNIKPLYDDYIGALRAIDPATGKIVWEYKNYSPLWGGVLTTAGNLVFTGTPEGF
                     LKAFDAKTGKELWSYQTGSGVVGCPVTWEMDGEQYIAVPSGWGGAVPLWGGEVAKRIS
                     YLTQGGSLWVFKLHKS"
     gene            7980..8831
                     /locus_tag="PK144_03660"
     CDS             7980..8831
                     /locus_tag="PK144_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNF64853.1"
                     /translation="MKRILAAAVLAGLAVAQGVTSASADALAEVREKGVLRVAVYKDF
                     PPYSYTDAQGRMTGLDVEIGKALAGKMGVAMALVPIPASDESMEDDLRNAVWKGHYMG
                     WGKADVMLHVPVNADFQKRVDKVALFAPYYRELVAVAYDKTKIPDLNSLDVFRTEKVG
                     VEMRTVADQYLITTSNHAYINNVRHFRTVDAACEAFKAGEVPAFAAQRGQLEHCLGPD
                     RSRFVIGTVPAPDVRVFEWNVGMAVKADETGLRDALGEAMEALRKDGSLQKIFDRQAM
                     SLNTPAN"
     gene            8885..9631
                     /locus_tag="PK144_03665"
     CDS             8885..9631
                     /locus_tag="PK144_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNF64854.1"
                     /translation="MPVAVLCAALLATVAHAGPGVPADPLASPQWPAMQQRFFRDATV
                     VFDDLVEVTLPPAAEDSMQVPVSARVRSGTAVDTLIAFIDFNPIPKVLEYRPLDAEPY
                     LGFRVRVQQATPIRVAARTADGVWHVGGAWVDAAGGGCTLPSANAAKVREEDIGHVAA
                     RRWPLDGIGSRVKLRIAHPNDTGLISGTPAFFVEALDFTAADGRRLAELRSFEPVAES
                     PVFVLELHTDGPVRIAGHDNTAGRIAGEAP"
     gene            9628..10587
                     /locus_tag="PK144_03670"
     CDS             9628..10587
                     /locus_tag="PK144_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNF64855.1"
                     /translation="MTARRKQGKQAGAVRAAALAFAVIVTVPLQAADTDYRLQPEPVA
                     PGTYLVAGVPENFSRSNGGNIANTAFVVTGAGVVVIDTGPSKRYGQQLRAAIARVTAE
                     PVVAVYLTHHHPDHALGNQAFADVPIRALPGTIEGLREQGNDFNSNLFRMVGDWMRGT
                     DPLLPAEAVAAGSVRIGTHEFELIPNAGHTAADLAILDRTTGTLFSGSVFNGRTPATP
                     NAVIADWIASLDRLAALNAERIVPAVGAITDNRAIAEDIDWLRWLDETLHEAAGRGAD
                     MAEVLRLPIPARFNDFALLREEYERSVSHLYPAIERASLGVRD"
     gene            10601..11218
                     /locus_tag="PK144_03675"
     CDS             10601..11218
                     /locus_tag="PK144_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HNF64856.1"
                     /translation="MDGAPVRFRDHAAVLCVPVLGLLCAAGPVMALQPPQMPELPLGT
                     LNPDGYRDHAWHAPVPATHPDATTVDTAEARTLLESGAALAVDVMAAVERPAAAGEPG
                     AFIPNRPRRHIPGSIWLPNVGYDELTPAQARWYAQALARLSRGERDRGLLIYCIADCW
                     LSWNAVHRAASFGYTHLYWYPEGSDGWAEAGLALVEARPEPPPPP"
     gene            11325..12314
                     /locus_tag="PK144_03680"
     CDS             11325..12314
                     /locus_tag="PK144_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF64857.1"
                     /translation="MPRTVSSLLLRACLLFAGGLCAFTATALETVRVGFQQSGTVQWE
                     LQLMQELGLAEREGVRLELVPLATKDATHIALQGGAVDVVVDDWLWVSRARDEGRPYT
                     CVTHSLAAGALYARPDSGITRLADLAGKKLGVAGGAVDKSWLLLRAYAQKTLGVDLAQ
                     QVEPQYGAPPLLNQLALRGELPAVLNYWNYGARLSAAGFREVISMRTVLTGLGAPPGL
                     PVIAWVFPDEFAAQHGETLRGFLRASRAAKQALATDDAVWERLRPLMQAANDTEFRSL
                     RDTYRQGIPQHVGPQDALTASRIFALLARLGGEALVGRGTTLATGTFWTGYAD"
     gene            12304..13071
                     /locus_tag="PK144_03685"
     CDS             12304..13071
                     /locus_tag="PK144_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF64858.1"
                     /translation="MRTEPGLSRAAERRLRFAAFALLLLLWWGLALVLHSDVLPTPAA
                     VWERLWAHAVSGELWRHLGATLARVAASFLIAMLVGTAIGILLGGSRRLDVFFDGWLV
                     LGLNIPALVTIILCYVWFGLNDAAAVLAVAANKIPTVVVTVREGARAVDRELLAVATA
                     FRVPPLRRLTRVYLPQLLPYLFASARSGLSLIWKIVLVVELLGRPNGIGFRLNAFFQF
                     FDIAGILAYTLAFAAVVLAVEAWLLRPLERRLLGWRA"
     gene            13059..13814
                     /locus_tag="PK144_03690"
     CDS             13059..13814
                     /locus_tag="PK144_03690"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64859.1"
                     /translation="MARVSGGGLSVELQAKYWPGSDVPVLAGLRLDVAAGEFLCLVGP
                     SGCGKSTLLAAIAGLDADFLGRIDAPPERGMLFQTPRLMPWLTVRDNLALVLADGRCS
                     PAAEALLDAMEIPPALLDRYPGQLSGGQQRRVALARAFAVRPRLLLLDEPFVSLDAPV
                     AQRLRELLLEHWMRERPTVLFVTHDLDEALALGDRIVFLGGRPAGIVLELVLPEPRPR
                     APEAVWRLRRQLLSTHPELLSGRVIPDPETPRP"
     gene            13811..14581
                     /locus_tag="PK144_03695"
     CDS             13811..14581
                     /locus_tag="PK144_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF64860.1"
                     /translation="MSPASSRPPLAEGTPLLAVEHVVKAYGPRKALDGVSLEVGRGEF
                     VALLGPNGAGKSTLFQLLSGLFVADGGRIAVCGHSLAAAPAPALARIGVVFQQPTLDL
                     DLSVLANLRFHARLHGLGGARARERIDAELARLGLTERAHEPARALSGGNRRKVELAR
                     ALVHEPDVLLMDEATVGLDPASRRQLLDDVLALKQARGVGVLWASHLVDEAERADRVV
                     VLHRGHVLFTGTPAALVAERGAANLADAFLAMTGGASD"
     gene            14733..15710
                     /locus_tag="PK144_03700"
     CDS             14733..15710
                     /locus_tag="PK144_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HNF64861.1"
                     /translation="MNRMHPLAVALAAALLAAPAVAKDTGLVFISSEKDNAVTALDGK
                     TLEVVKVIPTGKRPRDLMFSPDHTKLYAACGDADRVDVIDVATLAVTGRIPVGEDPEM
                     FDLSPDGRTLYVSREEDSVLGIIDLATRKQTGEVKVGGEPEGVLVSADGRTVYVTSEV
                     ASLVSAVDPVAGKVVKTAKVGNRPRRFAMTPDGTQLWVTNELGASVSIIETAGHTVAA
                     TLKFEPKGFRADDVSPVGITMSADGRTAYVALGKANHVAVVDVASRAVQDYILVGKRA
                     WGLTLDRSGQRLFVANGLSDDVSVVDTATRKAVKTIRAGRVPHSIAIDD"
     gene            15729..16904
                     /locus_tag="PK144_03705"
     CDS             15729..16904
                     /locus_tag="PK144_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF64862.1"
                     /translation="MRRLRLAACLAAVLVVTPAIAAELAIGFLGLADDPRYERTRLEH
                     QFQGQPGGSPLAGAEVAIEESEFAAEAQGLKLRLDSAEAAGDDAASVRGAFDGLVAGG
                     AKFVLVDLPPAALDALAQATRTGGVTLINVSAGDDALRAARCQPQLLHTMPSEAMLAD
                     GLAQYLVSRKWRKVLVLVGPRPEDQLRLAAFTAAARRFGLKIVDTRSFRLSNDPRERD
                     LGNVALLTAGEDYDALYIADTDGEFARDANYRGQLPRPTVGSAGLAPEAWHWSWERNG
                     APQLNNRFLKHAKRPMRGADWAAWVAVKALVEAAGHVPDADPAKLLAYLRGPELVVDG
                     FKGARLSFRAWDGQLRQPVFLAFGNGVAASAPVDGFLHPVNALDTLGPDQPDTQCRR"
     gene            16978..>17402
                     /locus_tag="PK144_03710"
     CDS             16978..>17402
                     /locus_tag="PK144_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNF64863.1"
                     /translation="MSETLETLLTPMTLVRSTDRATLQRAQPYAEGDAITWLTAEPQR
                     LEARVQGSRPQPYVVEFAVRNDELSRRCNCPMGNEGGFCKHLAAVALAWLAGRAAAGL
                     TEGELPTIRRHLQSLPAAELVELLMQHAVEDQALRARLLR"
BASE COUNT         2409 a         6306 c         6255 g         2432 t
ORIGIN      
        1 cgggcgaggc ggcggcgcgc cagaccagcg ccgcgcagcg cgtgcggctg ccggcgggcg
       61 gtgcctgcgc ggcgacggcc gcggcttcga gcagcagcca gtcggcggcg accggatcgg
      121 agaccagccg cgcgccgtgc tcggcggcga gccggcgcag ctgcgcgccg accgggccgg
      181 gcggcccctg gatgcagagg gtgcgcccgg cgagcagcgg catcgccggg gcgggttcgt
      241 cggcttcgag cgcgagacag gccgtgaagc ggctgccgcg gccggggctg ctatcgacct
      301 cgatcgtgcc gcccatggcc tcggcgagcc gccgggcgat ggtcaggccg aggccggagc
      361 cgccgtagcg gcgcgtcatc gagctgtcgc cctgcgcgta ggcatcgaac agcttctcga
      421 tgcgcgccgg atcgatgccg atgccggtgt cctcgacggc gaggcagagc tgcgcgcagc
      481 cctcgcgcgt gcgcaccgac acctcgacgc cgacccgccc ctgggcggtg aatttcacgg
      541 cgttgtcgac gaggttgccg aggatctgct gcacgcgcag cggatcgccg agccagcgcg
      601 ccggcgtgcc cggagccgcg tcgatcgtga attccagccc ctgcgcgcgc gccgcgccgg
      661 tgtgctggtc ggcgacggcc gacagcaccg tgtccagcgt gaaatgcgtg cgttcgagtg
      721 acaggccgcc ggcctcgatg cgcgaggcat cgaggatgtc atcgaccagc gccagcagcg
      781 tcgcgccggc cccgcgcagc cgcgtcagcc agtcgcgctg cagcggatcg acggcccggc
      841 gttcggcgag gcgcgcaagc ccgagcaccg cgttcagcgg cgtgcggatc tcgtggctca
      901 tgttggtcag gaacacgctc ttggcggcgg cggcggcgct ggccgcgcgc agcgcctcgg
      961 cgagcgcggc ctcggcgtgc ttgcgcttgt cgatgtcgag gtgcgtgccg aaggcgcgca
     1021 gcggccggcc gtcggcgtcg cgctccagca cctggccgcg gtcgagaatc cacttccagc
     1081 cgccgtcgcg gcagcgcagc cggtattcgg cctcgtagta gtcggtcccg ccggcgtaat
     1141 ggagcttcag cgcggcgtgc acggcctcgc ggtcctcggg gtgcaggcgc tgttcccatt
     1201 catcgagcgt gtcgccgatc tcgtcatcgg cgtagccgag catttccttc cagcgcggcg
     1261 aatagaagat gcggtcggtt tcggcgttcc agtcccagac gccgaggccg ctgccttcga
     1321 gcgcgtagtg ccagcgctgc tcggccgcgg cgagggcctg ctcgatgtcc ttgcgcgccg
     1381 agatgtcgac catctgcgcg gtgaagtgca gcggccggcc ggcggtgtcg cgcacgatcg
     1441 ccaccgacag cagcacccag accgcgcggc cgtcgcggcg cagatagcgc ttttccagcg
     1501 tgtagctctc gcgctcgccg gtcagcagcg cctcgaccag cgcgaggtcg cgctcgaggt
     1561 cgtccggatg cgtgatcgcg cggaagttgc gcccgagcag ctcggattcc tcgtagccgg
     1621 tgatcagcgc cagctcgcgg ttgacgcgct ggaagcggcc ttcgagatcg accaccgcca
     1681 tgccgatcgg cgcgtgcgcg aaggtgcggc tcagggcctc ctcgctgttg cgcagcgccg
     1741 cctcggtgcg ccgccggtcg gcgatgtcgc ggatcgagcc ggcgacgcgc atggcgcgcc
     1801 cggcgccgtc gcgccgcgcg agcgcgtgca gctcgaccca cagccgcgtg ccgtcgtcgc
     1861 ggcgcatgcg cagctcgatc tgcacggccg gcgtgcgccc gctcaggtgc gcggccagcg
     1921 cctcgtcgta gcgcggccgg tcggccggat cgatgcgttc gcgcagcgcc gcgccgctgg
     1981 ccggtcgccc tgcgtcgctg cagccgagca gcgtgcacag gcgcggggac aggaacagcg
     2041 cgtcctcggc cggcagccat tcccagagcc cgtcctgcgc ggcctcgacg gcgagcgcgt
     2101 agcgctcctc ggactcgatc agcgcccggt gcgcggcgcg cagcggcgtc gtctcgatca
     2161 gcgcgcagac cagcccggcc tgcggatcgt cggcgtcgat gcgcgtgatc tgcagcagcc
     2221 acacggcgcg gtcgctgtgc agttcatcgg catagcggcc gccgaccgtc agcacggtct
     2281 ccagccggcg caccaggccc ggaaacgtcg ccagcccgtc cagcccgctt gcgggccgcc
     2341 cgagcgcgtc gtccgacagc ccgaacagcc gggcggcgcg cgcgctgtag cggcgcaggc
     2401 agagcgtgcc gtcgaaggtc agcagcgcct ggtcgagaca gtcgagggtc gcggccagct
     2461 cgcggtcgag cgcatcgacg cgggcggcgc ggccttcggg ggtatcggca tcaggaccca
     2521 cagattctct ccgcatgtca tccggatacg gtgccggcgc gagggcgcgc cggcgcgcgg
     2581 agtctaaacc gtgcggccgt gatcggcgca gccgccgcaa gtggcagtca ggcccggcag
     2641 ggcaggggac gaagggaagg gcacgcgcgg ctcagcgggc gccagcgagc gggcggcgcg
     2701 gcagcgtcag gcgggcttcg aggccggggc catcgctgcg gttggcgagc gtcagctcgc
     2761 cgccatggcc gcgggcgatg gcgcgggcga tcgacagccc gaggccggtg ccgccggtgg
     2821 cgcgcgagcg cgagtgttcg aggcgccgga acggttcgaa cacgcgttcg agttcggccg
     2881 ccggcaggcc ggggccgcgg tcgcggatgc agatgcacag cgcctgcggc gcatcgtgca
     2941 gcacgatctc gaccggggcg tcggcctcgc catcgctgtt gccgtagcgc agcgcgttgt
     3001 cgagcaggtt ctgcaggcag cggcgcagcg cccgcgggcg tgccggcagc ggtgcgccgc
     3061 cgtcgccgag cacctcgacc gccgcgccgg tgtcggcggc atcgtcggcg agcgcctcgg
     3121 cgagggcgcc gatcgcgacc ggcaccgtcg cctcctcgcg cccggcgcgc aggaagtcga
     3181 gcgtgtcggc gaccgtggtt tcgagatcgg cgagatcggc gagcgcgcgc gcctgcacgc
     3241 cggattcgcc gatcagctcc aggcgcaggc gcaggcgcgc cagcggcgtc ttcaggtcgt
     3301 gcgagacggc ggcgagcagc tggtcgcgct cggcgagctg gcggcgcagc cgcgcctgca
     3361 tgtcgttgaa cgcgatcgcc gcgtcgcgca cctcgaccgg cccggtttcc ggcagcggcg
     3421 gccgctcgag atcggtgccg agctcgcgcg ccgcgccggc gagccgcgcc agcggccggg
     3481 tcagcccgcg cacggcgagc accgacaccg caagcacgat ggcgctgacc agcgccagtg
     3541 ccgccagcag ccgcaccggc tgcgcgccga tgaagcgcgg cagcacgtac aggaagctga
     3601 cggtgccgcc atcggccagc ccgacctgca cgaagaagct cggcgcccgc cggcccttgc
     3661 cgtcgtcctc gtccctgccg ctgccggcgg cctcgccgtg gctgaggctg accgccagcg
     3721 gccggtcgtc gccgagctgc gcgtgcagcg cgccggcgag tgccgcggcc tgcggcgaga
     3781 cctcggccgg gttgccggcc caggcgagat cgaggcggat gtacagcggc ggcaggtcga
     3841 gcgcatcgac gacgcgcgcg cggtcgccct cgtcgaggcc gtcgagcagt tcgacgaggt
     3901 tggcgatgcg ctcggcgttg tcggtgacga gcacgcggta cagcgcctcg ccgcgatcgg
     3961 tgagcgtcag ccaggcgctg agcgcgagcg cggcgccgag gccggcgagc agcagcagca
     4021 gcaggcggcc gaacaggctg gccggagcca ggcgcaggcg gcgggggcgg tgcggcatcg
     4081 gcgcgcgctc agccgtattc gcgcgtgacc ggaccggtca gcaggtagcc ctcgttgcgc
     4141 acggtgcgga tgatcgccgg ctccttcgga tcgtcgccga ggcggcggcg gatgcggctg
     4201 accagcatgt cgacgctgcg gtcgaagggc ccggcctcgc ggccctgggt caggtcgagc
     4261 aggcggtcgc gcgacagcac gcgtccgccg tgctcggcca gcgccttcag cagccggtac
     4321 tcggccgagc cgagcgcgac gatcgtgccc tgcggatcga cgagctggcg cgcggcgaca
     4381 tcgagcgtcc agtcatggaa gcggaatgcg gcgatctcgc cgcgcgccgc gccgccggct
     4441 gcggcggggt tcgcggccgg tcccgggttc tgcgcacggc gcagcacgct gcggatgcgc
     4501 gccagcagct cgcgcgggtt gaagggcttc ggcaggtagt cgtcggcccc catctccagg
     4561 ccgacgatgc ggtcggtgtc gtcgccgcgc gcggtcagca tgatcaccgg cagcgccgag
     4621 cgcgcgcgca gcgagcggca gaggctcagg ccgtcctcgc ccggcagcat caggtcgagg
     4681 atcaccagct cgatgccgcc ggcggcgagg gccgcgtgca tcgcggtccc gtcaccggcc
     4741 tcgctgacgc gcaggccgtt gcggccgagg taatccgcca gcaggcggcg cagttcgggg
     4801 tcatcgtcga cgatcaggat gtgctcgttc ataagcgcct tttatatgcg ttcggtgcgc
     4861 gcggcggcag cggtggttac gcatggttac gcccggccga cgtgccgatg cgtgcgggtg
     4921 acggcagcga cgttgaatga gcagcgtcga aaacaggcag gtcccgagga ggatgccgac
     4981 atgagcccga acttccatga ccgtcacccc aatccgtccc cggcgctgcg ccggcgcagc
     5041 gcgctgctgg tcgccgcgct cgctgcgacg ctggcgatgc cgctcgccca tgcccggccg
     5101 ccgatggacg gcccggacga tggccccggc atgcacgagg gccgcgatcc ggcccggcac
     5161 tgggcgcgca tgcacgagcg catggagcag cgcttcgagc agctcgaaca ggcgctgaag
     5221 ctgcgccccg agcagaaacc ggtctgggac gccttcgtcg ccgcccgccc gcagccgccg
     5281 cagcgctcgc cggaggaatg gcgccagtgg cgtgagcaga tgcagaccat gggtgcgatc
     5341 gagcgccaca aggcgatgga gcagcgtctg gaggagcgca tgcagcagat gaaggcgagc
     5401 ggcgtggcgc tgcagaagct ctatgacgtg ctcgacacca gccagcgggc gacgctgggc
     5461 cgcttccagt ggtggccggg gccgaagggc atgaaggacg gttacggccc gggcccggcg
     5521 cgcacgccgg ccccgccgcc ggccgccccg gtcgccccgg ctgctccgcc cgcccagtaa
     5581 gagcggacgc acccgtcccc cgagcggccg tcgccctgtg cttgcagggc ggcggccgcc
     5641 ttgcgtccgg cgggcgggaa atcctcccgg cctgcaggcg ccggctggcg atccggccgc
     5701 cgggcgccga tgattgccgg ccgctccctg ccggcgctgc ggaccgtgcg ggcggcatgt
     5761 gcagccgccg gccggggcag tgccgtgcgg gggacaggct gtaccgcgct gtcacgatcc
     5821 gcgccaccgg tgcggcaagg ctgtgacgcc cgcatcgcat gctgcattgc ggcaattgcg
     5881 tgaccggaaa aatccggcaa tcgcacgatc cgtgcaaacg attgatcgct accggattgc
     5941 tgcaattgca cggaacgcgc acgaaaaaaa tggcattgac cttgctcaag tgcgatgcac
     6001 cgctcatttg atgtcacaga agcgggacat tcgaatctcc acgcccgaag gagccttgag
     6061 gagcaatccc atgatgcatg cacgattccc gatgaaccgc cttgccctcg gtgtctccct
     6121 gctgctcggt gtcgcttcgg cggccgtcgc cggcgtcacc gaccagaacc tgctcgatgc
     6181 cggcgtcaag gacaacaaga gcgtcctgac ctggggcatc gactatcagg gcacgcgcta
     6241 cagcccgctg aagcagatca acgccgacac gatcaaggac ctcgtaccgg cgtggacctt
     6301 ctcctacggc gacgagaagc agcgcggcca ggaagcccag ccgctggtct acaacggcaa
     6361 gatcttcgtc accgcctcgt actcgcgcat ctacgcggtc gatgccaaga ccggcaagaa
     6421 gctctgggtc tacgagcacc gcctgccgga cggcatcctg ccgtgctgcg acgtgatcaa
     6481 ccgcggtgcc gcgctctacg gtgatctggt catctactcg acgctcgatg cgcagctcgt
     6541 cgcgctgaac caggagaccg gcaaggtcgt ctggcgcgag aagatggaca actacaagga
     6601 cggctactcg aactcggccg cgccgatcgt cgtcaagggt ctggtcatca ccggcgtctc
     6661 gggcggcgag ttcggtgtcg tcggccgcgt cgatgcgcgc gatgcgctga ccggcaagct
     6721 ggtctggacg cgtccggtca tcgaaggcca catgggttac accttcaagg acggccagaa
     6781 gaccgaggcc ggcctcaccg gtgaaaccgg caagacctgg ccgggcgatc tgtggcagac
     6841 cggcggcggc gcgacctggc tcggcggcta ctacgatccc gagaccgact cggtcctgat
     6901 cggcaccggc aacccggcgc cgtggaacgc ccacacccgt ccgggcgaca acctgtactc
     6961 ggcttcgagc ctcgcgatcg acccgtccac cggcaagatc aagtggcact accagacgac
     7021 cccgaacgac ggctgggact tcgacggcgt caacgagctg gtctcgttcg acctcaagaa
     7081 ggacggcaag accatcaagg ccgtcggcaa ggctgaccgc aacggcttct tctacgtgct
     7141 cgaccgcacc aacggcaagc tgatcagcgc cacgccgttc gtgaccaagc agacctgggc
     7201 gaccggcgtc gacctcaaga ccggccgtcc gatcgagacc ggcgtgcgcc cgggcgatcc
     7261 gtccaagagc gccgacggca agaagggcga gaccgtgttc tcggcgccgt ccttcctcgg
     7321 tggcaagaac tggatgccga tggcctacaa caaggacacc ggcctgttct acgtgccggc
     7381 caacgagtgg ggcatggaca tctggaacga gccgatcacc tacaagaagg gtgcggccta
     7441 cctgggtgcc ggcttcaaca tcaagccgct gtatgacgac tacatcggcg cgctgcgcgc
     7501 gatcgatccg gccaccggca agatcgtctg ggaatacaag aactactccc cgctctgggg
     7561 cggcgtgctg accaccgccg gcaacctggt gttcaccggc acgccggaag gcttcctgaa
     7621 ggccttcgac gcgaagaccg gcaaggagct ctggagctac cagaccggct ccggcgtggt
     7681 cggctgcccg gtcacctggg agatggacgg cgagcagtac atcgccgtgc cctcgggctg
     7741 gggtggtgcg gtgccgctgt ggggtggcga ggtcgccaag cgcatcagct atctgacgca
     7801 gggcggcagc ctctgggtgt tcaagctgca caagtcctga tcgtccggtt acacgcgggc
     7861 gtgaacgggg tgcggcgcga ctgccgcacc ccgctttctg ttgcagggac gccgtctcag
     7921 ccgtgccggc gcgccgggat gaccggcccg ccgctgcgcc gatctcccgg gagcctgaca
     7981 tgaaacgtat cctcgccgcc gccgtgctcg ccggcctcgc cgttgcccag ggcgtcacct
     8041 cggccagtgc cgatgcgctc gccgaagtgc gcgagaaggg cgtgctgcgc gtcgccgtct
     8101 acaaggactt tcccccgtat tcctacaccg acgcccaggg ccgcatgacc ggtctggatg
     8161 tcgagatcgg caaggcgctt gccgggaaga tgggcgtcgc gatggcgctg gtgccgatcc
     8221 ctgcctccga cgaatcgatg gaggacgacc tgcgcaacgc cgtctggaag ggccactaca
     8281 tgggctgggg caaggccgac gtgatgctgc acgtgccggt caacgccgac ttccagaagc
     8341 gcgtcgacaa ggtcgcgctg tttgcgccgt actaccgcga gctggtcgcg gtcgcctacg
     8401 acaagacgaa gattccggac ctgaactcgc tcgacgtgtt caggaccgag aaggtcggcg
     8461 tcgagatgcg cacggtcgcc gaccagtacc tgatcacgac ctcgaaccac gcctacatca
     8521 acaacgtccg ccacttccgc accgtcgatg ccgcctgcga ggccttcaag gccggcgagg
     8581 tgccggcctt cgcggcgcag cgcggccagc tcgaacactg cctcggcccg gaccgctcgc
     8641 gcttcgtgat cggcacggtg ccggcgccgg acgtgcgcgt gttcgagtgg aacgtcggca
     8701 tggcggtcaa ggccgacgag accgggctgc gcgatgcact cggcgaggcg atggaggcgc
     8761 tgcgcaagga cggctcgctg cagaagatct tcgaccgtca ggcgatgagc ctgaacacgc
     8821 cggccaactg agcccgccgc gggcctcgat gcagggcgtc gccgtgccgt tctcatccgc
     8881 tgtcgtgcca gtcgccgtcc tgtgcgcggc gctgcttgcc acggtcgcgc acgccggtcc
     8941 cggcgtgccg gccgatccgc tcgcctcgcc gcagtggccg gcgatgcagc agcgcttctt
     9001 tcgcgacgca acggtcgtgt tcgacgatct ggtcgaggtc acgctgccgc cggcggccga
     9061 ggactcgatg caggtgccgg tcagcgcgcg cgtgcgcagc ggcacggccg tcgacacgct
     9121 gatcgcgttc atcgacttca acccgatccc gaaggtgctc gaataccggc cgctcgatgc
     9181 cgagccgtat ctgggctttc gcgtgcgcgt gcagcaggcc acgccgatcc gggtcgccgc
     9241 gcgcacggcc gatggcgtct ggcacgtcgg cggcgcctgg gtcgatgccg ccggcggcgg
     9301 ctgcacgctg ccgtcggcaa acgccgcgaa ggtgcgcgag gaggacatcg gccacgtcgc
     9361 cgcgcggcgc tggccgctcg atggcatcgg ctcgcgcgtg aagctgcgca tcgcgcaccc
     9421 gaacgacacc gggctgatca gcggcacgcc ggccttcttc gtcgaggcgc tggacttcac
     9481 ggccgccgac ggccgccggc tcgccgagct gcgcagcttc gagccggtcg ccgagagccc
     9541 ggtgttcgtg ctcgaactgc acaccgatgg cccggtgcgc atcgccggcc acgacaacac
     9601 cgccggacgc atcgccggag aagcgccttg actgcccgaa ggaagcaggg gaagcaggca
     9661 ggagcggtgc gcgcggcggc gctcgcgttc gcggtcatcg tcaccgtgcc gctgcaggcc
     9721 gccgacaccg actaccggct gcagcccgag ccggtcgccc ccggcacgta tctggtcgcc
     9781 ggcgtgcccg agaacttctc gcgcagcaac ggcggcaaca tcgccaacac cgccttcgtc
     9841 gtcaccggcg ccggcgtcgt cgtcatcgac accgggccgt cgaagcgcta cggccagcag
     9901 ctgcgcgcgg cgatcgcgcg cgtcaccgcc gagccggtcg tcgccgtcta cctgacccat
     9961 caccacccgg accacgcgct cggcaaccag gccttcgccg acgtgccgat ccgtgccctg
    10021 ccgggcacga tcgagggcct gcgcgagcag ggcaacgact tcaattcgaa cctgttccgc
    10081 atggtcggcg actggatgcg cggcaccgat ccgctgctgc cggccgaggc cgtcgccgcc
    10141 ggcagcgtgc gcatcggcac tcacgagttc gagctgatcc cgaacgccgg gcacacggcc
    10201 gccgatctcg cgatcctcga tcgcacgacc ggcacgctgt tctcgggcag cgtgttcaac
    10261 ggccgcacgc cggcgacgcc gaacgccgtg atcgccgact ggatcgcgag cctcgaccgg
    10321 ctggcggcgc tgaacgccga gcgcatcgtg ccggcggtcg gggcaatcac cgacaaccgc
    10381 gcgatcgccg aggacatcga ctggctgcgc tggctggatg aaaccctgca cgaggccgcc
    10441 gggcgcggcg ccgacatggc cgaggtgctg cgcctgccga tcccggcgcg cttcaatgac
    10501 ttcgcgctgc tgcgcgagga gtacgagcgc tcggtctcgc acctgtaccc ggcgatcgag
    10561 cgcgcctcgc tcggcgtgcg cgactagggt gccggccccg gtggacggtg cgccggtgcg
    10621 cttccgcgat catgccgccg tgctctgcgt gccggtgctc ggcctgctgt gcgccgccgg
    10681 cccggtcatg gcgctgcagc cgccgcagat gccggagctg ccgctcggca cgctgaaccc
    10741 ggacggctac cgcgaccacg cctggcacgc gccggtgccg gccacgcatc cggacgccac
    10801 cacggtcgac accgccgagg cgcgcacgct gctcgaatcc ggcgcggcgc tggcggtcga
    10861 cgtgatggcc gccgtcgaac gtccggccgc agccggcgag cccggcgcct tcatcccgaa
    10921 ccggccgcgc cggcacatcc ccggctcgat ctggctgccg aacgtcggct acgacgagct
    10981 gacgccggcg caggcgcgct ggtacgcgca ggcgctggcg cgcctgagtc gcggggagcg
    11041 cgaccgcggc ctgctgatct actgcatcgc cgactgctgg ctgtcgtgga acgccgtgca
    11101 tcgcgccgcc agcttcggct acacgcacct gtactggtat cccgagggca gcgacggctg
    11161 ggccgaggcc gggctggcgc tggtcgaggc ccggccggaa ccgccaccgc caccgtgagc
    11221 cgccatgccg gcgcggcagt ggccgcggct tcccgttcgc ccttaccctc cgcccggacc
    11281 tgcccgacgg caccgcgccg ccgcccgacc tgacggatcg atcgatgccc agaaccgtat
    11341 cctcgctgct gctgcgcgcc tgcctgctgt tcgccggcgg cctgtgcgcg ttcaccgcca
    11401 ccgcgctcga gaccgtgcgc gtcggcttcc agcaatccgg caccgtgcag tgggagctgc
    11461 agctgatgca ggaactcggc cttgccgaac gcgagggcgt gcgcctcgaa ctggtgccgc
    11521 tcgccaccaa ggacgccacg cacatcgcgc tgcagggcgg tgccgtcgat gtcgtcgtcg
    11581 acgactggct gtgggtgtcg cgcgcccgcg acgagggccg accctacacc tgcgtcacgc
    11641 attcgctcgc ggccggcgcg ctgtacgcgc ggcccgacag cggcatcacc aggctcgccg
    11701 atctcgccgg caaaaagctc ggcgtcgccg gcggggcggt cgacaagagc tggctgctgc
    11761 tgcgcgccta cgcgcagaag acgctcggcg tcgatctggc gcagcaggtc gagccgcagt
    11821 acggcgcacc gccgctgctg aaccagctcg cgctgcgcgg cgagctgccg gcggtgctga
    11881 actactggaa ctacggcgcg cgcctctcgg ccgccggttt ccgcgaggtg atctcgatgc
    11941 gcacggtgct gaccggcctc ggcgcgccgc ccggcctgcc ggtgatcgcc tgggtgttcc
    12001 cggacgagtt cgccgcgcag cacggcgaga cgctgcgcgg cttcctgcgc gcctcgcgcg
    12061 ccgccaagca ggcgctcgcg accgacgatg cggtctggga aaggctcagg ccgctgatgc
    12121 aggcggcgaa cgacaccgaa ttccgcagcc tgcgcgacac ctaccgccag ggcatcccgc
    12181 agcacgtcgg gccgcaggat gcgctgacgg cctcccgcat cttcgcgctg ctcgcgcgcc
    12241 tcggcggcga ggcgctggtc ggcaggggca cgacgctcgc caccggcacg ttctggacgg
    12301 ggtatgcgga ctgaacccgg cctgagccgc gccgccgaac gccggctgcg cttcgccgcc
    12361 ttcgcgctgc tgctgctgct gtggtggggg ctcgcgctcg tgctgcattc cgacgtgctg
    12421 ccgacgccgg cggcggtctg ggagcggctg tgggcgcatg ccgtcagcgg cgagctgtgg
    12481 cgccacctcg gcgcgacgct ggcgcgggtc gcggcgagct tcctgatcgc gatgctggtc
    12541 ggcaccgcga tcggcatcct gctcggcggc tcgcgccggc tcgacgtgtt cttcgacggc
    12601 tggctggtgc tcgggctgaa catcccggcg ctggtgacga tcatcctctg ctacgtctgg
    12661 ttcggcctga acgacgcggc cgccgtgctc gcggtcgccg ccaacaagat tccgaccgtc
    12721 gtcgtcaccg tgcgcgaagg cgcgcgcgcc gtcgatcgcg agctgctcgc ggtcgcgacg
    12781 gcgtttcgcg tgccgccgct gcgccggctg acgcgcgtct acctgccgca gctgctgccg
    12841 tatctgttcg cgtcggcgcg ctcagggctg tcgctgatct ggaagatcgt gctggtggtc
    12901 gagctgctcg ggcggccgaa cggcatcggc ttccggctca acgccttttt ccagttcttc
    12961 gacatcgccg gcatccttgc ctacacgctg gccttcgcgg ccgtggtgct cgcggtcgag
    13021 gcctggctgc tgcggccgct ggagcggcgt ctgctcgggt ggcgcgcgtg agtggcggcg
    13081 ggctgtcggt cgagctgcag gccaagtact ggccgggcag cgacgtgccg gtgctcgccg
    13141 gtctccggct cgacgtggcc gccggcgagt tcctgtgtct ggtcgggccg agcggctgcg
    13201 gcaagagcac gctgctcgcg gcgatcgccg gactcgatgc cgacttcctc ggccgcatcg
    13261 acgcgccgcc cgagcgcggc atgctgttcc agacgccgcg gctgatgccg tggctgacgg
    13321 tgcgcgacaa cctcgcgctg gtgctggcgg acggccgctg ctcgccggcg gccgaggcgc
    13381 tgctcgatgc gatggagatt ccgccggcgc tgctggatcg ctacccgggc cagctttccg
    13441 gcggtcagca gcggcgcgtg gcgctcgcgc gcgccttcgc cgtgcgcccg cgcctgctgc
    13501 tgctcgatga gcccttcgtg tcgctcgatg cgccggtcgc gcagcgcctg cgcgagctgc
    13561 tgctcgaaca ctggatgcgc gagcgcccga ccgtgctgtt cgtgacccac gacctcgacg
    13621 aggcgctggc gctcggcgac cgcatcgtct tcctcggcgg ccggccggcc ggcatcgtgc
    13681 tggaactcgt cctgcccgag ccgcgaccgc gggcgcccga agccgtgtgg cggctgcgcc
    13741 ggcaactgct ttcgacgcac ccggaactgc tgtccggtcg cgtcataccc gatccggaga
    13801 ccccgcgccc atgagccccg catcctcccg gccgccgctc gccgagggca cgccgctgct
    13861 cgcggtcgag cacgtcgtca aggcctacgg gccgcgcaag gcgctcgacg gcgtcagcct
    13921 cgaagtcggc cgcggcgagt tcgtcgcgct gctcggtccg aacggcgccg gcaagtcgac
    13981 gctgttccag ctgctgtcgg ggctgttcgt cgccgatggc ggccgcatcg ccgtctgcgg
    14041 ccacagcctc gccgccgccc cggcgccggc gctcgcgcgc atcggcgtgg tgttccagca
    14101 gccgacgctc gatctcgatc tgtcggtgct cgccaacctg cgcttccacg cgcggctgca
    14161 cggcctcggc ggcgcacggg cgcgcgagcg catcgacgcc gagctggccc gcctcggcct
    14221 gaccgagcgc gcgcacgagc cggcgcgggc gctgtccggc ggcaaccgcc gcaaggtgga
    14281 gctggcacgc gcgctggtgc acgagcctga cgtgctgctg atggacgagg ccacggtcgg
    14341 cctcgacccg gcctcgcgcc ggcagctgct cgatgacgtg ctggccctga agcaggcgcg
    14401 cggtgtcggc gtgctgtggg cctcgcatct ggtcgatgag gccgagcgcg ccgatcgcgt
    14461 cgtcgtgctg caccgcgggc acgtgctgtt caccggcacg ccggcggcac tggtggccga
    14521 acgcggcgcc gcgaacctcg ccgatgcctt cctggccatg accggcgggg ccagcgactg
    14581 aacgtccgcg ctccgggcgg actttccggg aattcttccc gaatcgccgg cctgcgggtt
    14641 tgcagggcgc gccggcggcg gcaaggtgcg cagtcccgca gacgcatcca ccccaggaaa
    14701 acccggatca gacctccgga ggagacccgc cgatgaatcg catgcatccg ctcgccgtgg
    14761 cgctcgccgc cgcgctcctg gccgcgccgg ccgtcgcgaa ggacaccggc ctggtgttca
    14821 tcagcagcga gaaggacaac gccgtgaccg cgctcgacgg caagacgctc gaagtcgtca
    14881 aggtgatccc gaccggcaag cgcccgcgcg acctgatgtt cagccccgat cacacgaagc
    14941 tctacgccgc ctgcggcgat gcggaccgcg tcgacgtgat cgacgtggcc acactcgccg
    15001 tcaccgggcg catcccggtc ggcgaggacc cggagatgtt cgacctgagc ccggacggca
    15061 ggacgctgta cgtgtcgcgc gaggaggatt ccgtacttgg catcatcgat ctcgcgacca
    15121 ggaagcagac cggcgaggtc aaggtcggcg gcgagcccga gggcgtgctg gtgtcggccg
    15181 acggcaggac cgtctacgtg acctccgagg tcgcgagcct ggtctcggcc gtcgatccgg
    15241 tcgccggcaa ggtcgtcaag accgccaagg tcggcaaccg cccgcgccgt ttcgcgatga
    15301 cgccggacgg cacgcagctc tgggtcacca acgaactcgg tgccagcgtc agcatcatcg
    15361 agaccgccgg ccacacggtc gccgcgaccc tgaagttcga gccgaagggc ttccgcgccg
    15421 atgatgtctc gccggtcggc atcacgatga gcgccgatgg caggacggcg tatgtcgcgc
    15481 tcggcaaggc caaccacgtc gccgtcgtcg atgtcgccag ccgcgcggtg caggactaca
    15541 tcctggtcgg caagcgcgcc tggggcctca cgctcgatcg cagcggccag cgcctgttcg
    15601 tcgccaacgg cctcagcgac gatgtctcgg tcgtcgacac cgccacgcgc aaggccgtca
    15661 agaccatccg cgccgggcgc gtgccgcaca gcatcgcgat cgacgactga acccggagaa
    15721 cccgccccat gcgccgcctg cgcctcgccg cctgtcttgc tgccgtcctc gtcgtcacgc
    15781 cggccatcgc cgccgagctg gcgatcggct ttctcggcct cgccgatgat ccgcgctacg
    15841 agcgcacgcg cctcgaacac cagttccagg gccagcccgg cggctcgccg ctcgccgggg
    15901 ccgaggtcgc gatcgaggaa tcggagttcg cggccgaggc gcaggggctg aagcttcggc
    15961 tcgacagcgc cgaggccgcg ggcgatgacg ccgcgagcgt gcgcggcgcg ttcgacggcc
    16021 tcgtcgccgg cggcgcgaag ttcgtgctcg tcgacctgcc gccggcggca ctcgatgcgc
    16081 tggcgcaggc gacgcgcacg ggcggcgtga cgctgatcaa cgtctcggcc ggcgacgatg
    16141 cgttgcgcgc cgcgcgctgc cagccgcagc tgctgcacac gatgccgtcc gaggccatgc
    16201 tcgccgacgg cctcgcgcag tacctggtgt cgcgcaagtg gcgcaaggtg ctggtgctgg
    16261 tcggcccgcg ccccgaggac cagctgcggc ttgcggcctt cacggccgcc gccaggcgtt
    16321 tcgggctgaa gatcgtcgac acgcgcagct tccggttgtc gaacgacccg cgcgagcgcg
    16381 atctcggcaa cgtcgcgctg ctgaccgccg gcgaggacta cgacgcgctc tatatcgccg
    16441 ataccgacgg cgagttcgcg cgcgacgcca actaccgcgg ccagctgccg cgcccgacgg
    16501 tcggctcggc cggactggcg cccgaggcct ggcactggtc ctgggagcgc aacggcgcgc
    16561 cgcagctcaa caaccgcttc ctgaagcacg cgaagcgccc gatgcgcggc gccgactggg
    16621 cggcctgggt cgcggtcaag gcgctggtcg aggccgccgg ccacgtgccg gacgccgatc
    16681 cggcgaagct gctcgcctac ctgcgcgggc cggagctggt cgtcgacggc ttcaagggcg
    16741 cgcggctgtc gttccgcgcc tgggacggcc agctgcgcca gccggtgttc ctcgccttcg
    16801 gcaacggcgt ggccgcgagc gccccggtcg acggcttcct gcacccggtc aacgccctcg
    16861 acacgctcgg cccggaccag ccggacacgc agtgccggcg ctgagccgcc acgcgcatct
    16921 cctgcggatg cggcatcatc cgcagcccgg ttttccgggc cgcaggagat cgtcgccatg
    16981 tccgaaaccc tcgaaaccct gctgacgccg atgacgctgg tgcgcagcac cgaccgcgcg
    17041 acgctgcagc gtgcgcagcc ctacgccgaa ggcgatgcca tcacctggct cacggccgag
    17101 ccgcagcggc tggaagcccg cgtgcagggt tcgcgcccgc agccctacgt ggtcgaattc
    17161 gccgtgcgca acgacgagct gagccgccgc tgcaactgcc cgatgggcaa cgagggcggc
    17221 ttctgcaagc acctcgccgc cgtggcgctc gcctggctgg ccgggcgcgc cgccgccgga
    17281 ctcaccgagg gcgagctgcc gaccatccgc cgccacctgc agtcgctgcc ggcagcggag
    17341 ctggtcgagc tgctgatgca gcacgccgtc gaggaccagg ccctgcgcgc gcgcctgctg
    17401 cg
//
LOCUS       DAOQIE010000034        17302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1532_length_17302_cov_7.610135, whole genome shotgun sequence.
ACCESSION   DAOQIE010000034 DAOQIE010000000
VERSION     DAOQIE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 17302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17302
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_17302_cov_7.610135"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK144_03715"
     CDS             <1..611
                     /locus_tag="PK144_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64864.1"
                     /translation="ASSPAGSYTRPATDSGEKAPMTAHAIGRGFALAAVLTTLPVTAW
                     ELRPAGKDPLSGQTRCLLVEEPLTINDGYGETRIDILFNGEALIVVTDSEIDAGFNDL
                     ALSVAIERRPPARGQKIVEPPPSPRFVPARIENKKTLVFTDAPAIEDAFKAGSTASFA
                     LRFWPLWPSVGSIPVEVSLKGFTKVAEEFSRCTGQPAAGTTR"
     gene            629..1501
                     /locus_tag="PK144_03720"
     CDS             629..1501
                     /locus_tag="PK144_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNF64865.1"
                     /translation="MGLRTKFNLVLILSFGIGIGLSGYLAYGLLRENAREEVLQNARI
                     MVEGALAIRGYTSREIQPLLTLPMKRQFLPQTVSSYAAQTNFRTLREHFPEYTYKEAA
                     LNPTNLNDRAADWEADIIREFRNNAERKEIITERETPTGRVLTLARPLKVGSPACLTC
                     HSTPDAAPDTMKAVYGTANGFGWQLNEIVGAQIVSIPMAVPLDRAEKVFHTFLAGLGA
                     IFLLLLVLLNLLLHFAVIRRVRRIATMADKVSLGEEDVPELDARGSDEIARLAASFTR
                     MRRSLENAMRLLGE"
     gene            1667..3079
                     /locus_tag="PK144_03725"
     CDS             1667..3079
                     /locus_tag="PK144_03725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNF64866.1"
                     /translation="MPVTIGKYRIESVLGHGAMGVVYRGHDPVLDRTVAIKTIRAELL
                     AAGDDGFEDDDWHARFRHEARAAARCTHPNIVTIYDFGETPDGSPYLVMEFVEGRELR
                     RHLGEGRQFDLPAAALILRQILRALDCAHLLGIVHRDIKPANVILLADGMVKVTDFGI
                     ARLSGGTGLTQTGAMVGTPNYMSPEQFTGGTLDRRADLYACAVIAYELLTGQRPYPGP
                     TPPALLHQILNTTPPRPSALRPSLPAALDAVLLRGLARDPGARFADACEFQRALDAAL
                     APALAPVPIHHHPEPGTVVMPAPLTLSDAELHEAETTLATHIGPLARVLVRRAAASAH
                     SRDAWLEEIARNISDDDERRRFLRRLRRGEPTGYTQYGTTAHTAHTGHVGHAGGNAAP
                     TAASGYGSSGSVTTVLRVDAATLATAQQALTQAIGPIARVLVRQTAPLAQDAADLYRR
                     LAERIDDADARRRFLATRPH"
     gene            3317..4570
                     /locus_tag="PK144_03730"
     CDS             3317..4570
                     /locus_tag="PK144_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF64867.1"
                     /translation="MSSSPAPAAVLAEPPLRSLAAFRHFWLTRVCTTIAYQMIGVAVG
                     WRVYELTGSALALGLIGLAQFLPALLLVLPAGQIADRHDRRQVAARAQRVSALAAALL
                     ALGSLGDWLTVHGIYALVLVIGAARAFEAPSLQALLPRLVPPARLGSAVAASASAMQT
                     AVITGPALAGFVYAAGPAAVFAASALLWTLASLQLARIEPLNRPATSAPPPGLDTLFA
                     GITFIRSRPVVLGAISLDLFAVLLGGATALLPIYARDILLTGPLGLGLLRSAPAIGAL
                     AMSLWLSRHPPQRGVGRLMFAAVAVFGVATLVFALSTQFALSLAALVVLGASDMISVV
                     IRASLVQLETPDEMRGRVSAVNSIFIGASNQFGEFESGLTAALFGTVPAAALGGLGTL
                     LVVLVWMRLFPDLRRRERLLETGTG"
     gene            4724..5458
                     /gene="aqpZ"
                     /locus_tag="PK144_03735"
     CDS             4724..5458
                     /gene="aqpZ"
                     /locus_tag="PK144_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNF64868.1"
                     /translation="MHFSKRLGAEFIGTFWLVLGGCGSAVLAAAFPQVGIGLHGVALA
                     FGLTVLTMAYAIGHVSGCHLNPAVTVGLTVGGRFPAQEVVPYVVAQVLGATAAAFVLY
                     LIATGKAGASIGGFAANGYGELSPGKYGLGAALITEVVMTAMFLFVIMGATDARAPAG
                     FAPIAIGLCLTLIHLISIPVTNTSVNPARSTSQALFAGSEYLGQLWLFWLAPIVGGAI
                     GALVYRAVCSELKPAVAPVEDLRPAA"
     gene            5584..6447
                     /locus_tag="PK144_03740"
     CDS             5584..6447
                     /locus_tag="PK144_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNF64869.1"
                     /translation="MSDARLRATLTGAVAILLWATLALGATLSAGLPPFELLALTFSI
                     GGLAGLAVVARGGRLARLRQPPAAFALGVAGLFGYHFFYFVALAHAPPVDASLINYLW
                     PLLIVLFSGLLPGERLQRHHLGGGLLGLAGAWVLISGGGTAPRLEYLPGYVSALACAF
                     VWSGYTVLNRRFAGLPTDGVAGQCLAVAVLAALCHAAWEPTVRPSAVQWAVIGWLGLG
                     PLGFAFYVWDHGSKHGDLQLLGVLSYATPVLSTLLLLAAGRGEPGWPVALACALIVGG
                     ALLASGRFRRG"
     gene            complement(6518..7090)
                     /locus_tag="PK144_03745"
     CDS             complement(6518..7090)
                     /locus_tag="PK144_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF64870.1"
                     /translation="MRKSPLLAAALAASLSLGFAGTAAAETFKVDPAHSFVNFEISHL
                     GFSMLQGRFNKMEGKFTYDAAKPGASTIEVRVDTGSVDSNYGERDKHLREKYLFTDKF
                     PSASFKSTKFSESGDKATLEGELTLHGVTKPVKVDVQFIGAGNDPWGGYRRGYVGTTT
                     IKRSDFGYTENLGPAADAVNLKLVIEGVRE"
     gene            complement(7213..7764)
                     /locus_tag="PK144_03750"
     CDS             complement(7213..7764)
                     /locus_tag="PK144_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNF64871.1"
                     /translation="MWRNSASAYGLTARLLHWSIAVLILGLIPLGWYITTLDYYDPGY
                     RLWPGLHRGFGVVVGLLALVRIGWAIADRAPPLAATLKPWEKLGAHAGHGLLYLATLL
                     IPLSGYLVSTAKGDPAELFGLISLPALWSGGEQWQEFVAGLHETLAWGTLAVVVVHAA
                     AALKHQFLDRDGTLARMTGRLPR"
     gene            complement(8031..8255)
                     /locus_tag="PK144_03755"
     CDS             complement(8031..8255)
                     /locus_tag="PK144_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64872.1"
                     /translation="MKTSMIIGVLLLVAGIAVLAYRGFNYTSEETVLQVGPVKATAET
                     EKHVGIPAWAGYTALALGGALVVVGATRRT"
     gene            8593..9075
                     /locus_tag="PK144_03760"
     CDS             8593..9075
                     /locus_tag="PK144_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64873.1"
                     /translation="MSEEISRDYVHGVYTELLAREGFRPKTDEDGDIQFRNQGFTTYI
                     RISEDDLPFLQMFIPSVYTLESGEEDTVALAVMAELNRQFKLVKVCRLDHSVAVLVEM
                     LLAQPEDLGGSLLRIADVLVVVAHEFHRLMSAALRERQRQAESELDEDDLPMPTRTVN
                     "
     gene            complement(9092..10654)
                     /locus_tag="PK144_03765"
     CDS             complement(9092..10654)
                     /locus_tag="PK144_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF64874.1"
                     /translation="MRLQIVFDDRVGIAMAVLNSLARHNINMTAGDIVTQNDGRGSFY
                     LHAAQMTLDELHRVLPEIRQVPGVRDVRPIPLMPGERKQSELRTLLGALPAPVLSLDL
                     EGRVVVANESAQRLLKLKGPDGQGTPLAPLVEDFDLVEHINRLDRPEYGVRLALRGQA
                     VLADFIPVLVPLARAGKSLSGAVMVLHGGSLEAASPAVDAGFASIVHRSAPMRALVAQ
                     ARRMAPLSAPILILGESGSGKARLAYACHLASPRREAPFLRLNAGELADAGAEAVLFG
                     VREQPGLFEQGAGGTLFLSDVAELSPALQARLLGFLQDGRFRRGGSVEPRRADVRIIA
                     STRVDLAAEVRDGRFRDDLYYHLHVLNLQMPSLRERQADVLPVALEAIEMAARQLACA
                     PPRLTPEAEAALLAHGWPGNVRQLENTLLRAVTVAEGGAITPELLALGTDATAASGAP
                     IDEALFEGSLAEALARVERLMLARLYPSHPSTRQLARRLGLSHTAVANKLRQYGIGLG
                     PGSGPAAEPRRG"
     gene            10979..12619
                     /gene="ipdC"
                     /locus_tag="PK144_03770"
     CDS             10979..12619
                     /gene="ipdC"
                     /locus_tag="PK144_03770"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HNF64875.1"
                     /translation="MNIALSLLHALKAHGAREIFGIPGDFALPFFRIVEESGVLPLYT
                     LSHEPGIGFAADAAARFNGALGVAAVTYGAGALNMVNPVAGAYAERSPLVVISGAPGA
                     AEVRSGMLLHHQVKTTDSQFAIYREITCAQTRLDDPATAPAEIARVLGACREQSRPVL
                     IEVPRDAVALPCAPVRPAAPAAYDREAVEACAGEILARLAAAARPTLLVDIEVRRYGL
                     EARVAELAHRLAIPVVTTFMGRGLLAEAAVPVAGTYLGVAGEPAVTALVEESDCLLML
                     GVLLSDTNFGVSGRQIDLRKAVYAADRQVQIGFHHYPDLPLGALVEALLALVSDLDPP
                     EPRLRPDLPPLPEAIVDGAPIAPDDIAAAIGTLMRRHGALPIASDIGDCLFTALGLAN
                     TALTAPGYYATMGFGVPAGLGIQATTGLRPLILVGDGAFQMTGWELGNCRRYGWDPIV
                     IVFNNASWEMLHVFQPESAFNRLDEWRFAELAAALGGHGERVRTRRELHAALAAAHAR
                     RGRFSLIEAMIAPGALSHTLARYVEGFRARRARQAAGH"
     regulatory      12843..13052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13207..15060
                     /locus_tag="PK144_03775"
     CDS             13207..15060
                     /locus_tag="PK144_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF64876.1"
                     /translation="MNTLHTGLAGLAALAALCPAPAAAADGDSSELVVTITGARSEQP
                     LAHATAPITVIDRTELDQSQAMTLNEALAGRNGIAAASNGGAGKVTNLYLRGANAGQT
                     LLLVDGIRLGSSTLGSPAWQLLPLGLAERIEVVRGAQSGLYGSDAIGGVVQVLTLPAP
                     GSPVRPWAEAGLGTQQGRHLGAGVRGSQGATTFSLGGDWLSSDGFDATATDTFAHNDD
                     RDGFRSGAGHVAVRQDVAPGQSFSFTALRAQGENRYDGSNPLTFATADHAEGKFVQQV
                     LALNADNRLTDAWRMKTTLGQSRDQNEDFADGLSAGAFDTRRDQFTWQHEFRLADGHL
                     LSLGAEWLHEKVGGSGVVDYAQKTRTTRALFLVDAVTLGAHDLQLSLRSDDIDDIGRH
                     NTGRLGWGWSFAPGLRATASYATGFKAPTFNDLYYPAGPFAAGNPDLEPESSHGWDLG
                     LEQHAGAWHWRLGGYRTRVGNLIQWVPDEFFRFSPVNVANARLTGIEASLGWQDHGWQ
                     VNLDADWLRAEDTDSGDPLPNRPNRSARLSIDYAPGTWSAGATLLAAAYPDDLPQRGW
                     SVLDLRSGYRLDKDFELKLKVNNVFDRDYQTVNGYPMPGREAWIAIEYGMR"
     gene            15312..15887
                     /locus_tag="PK144_03780"
     CDS             15312..15887
                     /locus_tag="PK144_03780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNF64877.1"
                     /translation="MGHRLSKIYTRTGDAGTTGLGDGTRVPKDALRIEVMGECDELNA
                     VLGMVLALPVREPIRAVLTEVQHRLFDLGGELCMPGYASLSAEDVTQLETDLDGFNAA
                     LPALKEFILPGGGPAAAACHLARTVCRRVERHLVTLVRTEAVNPHAPAYLNRLSDLLF
                     VLGRVLARDEGGSEVLWRNDHGRRRTAADQP"
     gene            15884..16204
                     /locus_tag="PK144_03785"
     CDS             15884..16204
                     /locus_tag="PK144_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNF64878.1"
                     /translation="MSFTENLASLPPAGHLAALELFEHGEPAGRIENRPGQAGSLRIY
                     HAVAVNGRIDAEAARRALGLYGEHLADAEANPGRHPNIDRLLALIRRNSVLVIRPVPA
                     ADGA"
     gene            complement(16238..17167)
                     /gene="ubiA"
                     /locus_tag="PK144_03790"
     CDS             complement(16238..17167)
                     /gene="ubiA"
                     /locus_tag="PK144_03790"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNF64879.1"
                     /translation="MKPSPEPAPAAPASGKPRAALCGPRLLAKAGDYAQLARMHRPIG
                     SFLLLWPTLWALWLAGHGQPPEGTVLVFCLGVFLMRSAGCVMNDIADRRFDPHVARTR
                     DRPLAAGRVTVREALWLAIGLSLVAFGLVLTQNGLTIGLSFAGLALAAGYPLMKRLHH
                     LPQVVLGMAFGWGIPMAFAAVTGSVPALAWLLYAANIVWSVAYDTFYAMTDREDDLKI
                     GVKSSAILFGARDRQIVALLQGAFLLLLGLAGLMAGCGPVYYLGLFAAAWLMAWEQYL
                     VRRREPAACFHAFLHNNWVGLAVFAGVLVDALP"
BASE COUNT         2456 a         6412 c         5992 g         2442 t
ORIGIN      
        1 ccgcatcgtc accggccggt tcgtatactc gaccggccac tgactccggg gagaaggcgc
       61 cgatgacggc acacgcgatc gggcgcgggt tcgcgctcgc ggcagttctg acgaccctgc
      121 cggtgacggc ctgggagctg cgcccggccg gcaaggaccc gctgagcggc cagacgcgct
      181 gcctgctggt cgaggaaccg ctcaccatca acgacggcta cggcgaaacc cgtatcgaca
      241 tcctgttcaa cggcgaggca ttaatagtcg tcaccgattc cgagatcgac gccggcttca
      301 acgacctggc gctgagcgtc gcgatcgagc gccggccgcc ggcgcgcggg cagaaaatcg
      361 tcgagccccc accgtctcca cgcttcgttc ccgcgcgtat cgagaacaag aagacgctgg
      421 tgttcacgga cgcaccggcg atcgaggatg ccttcaaggc cggcagcacc gcgagcttcg
      481 cgctgcgctt ctggccgctg tggccctcgg tcggctcgat cccggtcgag gtcagtctca
      541 agggcttcac gaaggtggcc gaagaatttt cccgctgcac cggccagccc gccgccggca
      601 ccacccgctg acagggggac gaacaccgat gggcctgcgt accaaattca acctggtgct
      661 gatcctgagc ttcggcatcg gcatcggtct ttccggctac ctcgcctacg ggctgctgcg
      721 cgaaaacgcc cgcgaggaag tcctgcagaa cgcgcggatc atggtcgagg gcgcgctcgc
      781 gatccgcggc tacacctcgc gcgagatcca gccgctgctg acgctgccga tgaagcgcca
      841 gttcctgccg cagaccgtgt cctcgtacgc cgcgcagacc aacttccgca cgctgcgcga
      901 gcacttcccc gagtacacgt acaaggaagc ggcgctgaac ccgaccaacc tcaacgaccg
      961 cgcggccgac tgggaagccg acatcatccg cgagttccgc aacaacgccg aacgcaagga
     1021 gatcatcacc gagcgcgaaa cgccgaccgg ccgcgtgctg acgctggccc ggccgctgaa
     1081 ggtcggcagc cccgcctgcc tgacctgcca cagcacgccc gacgccgccc cggacacgat
     1141 gaaggccgtc tacggcaccg ccaacggctt cggctggcag ctcaacgaga tcgtcggcgc
     1201 gcagatcgtc tcgatcccga tggcggtgcc gctcgatcgc gccgagaaag tctttcacac
     1261 cttcctcgcc ggcctcggtg cgatcttcct gctgctgctg gtgctgctga acctgctgct
     1321 gcacttcgcc gtcatccgcc gcgtacgccg catcgcgacc atggccgaca aggtcagcct
     1381 cggcgaggag gacgtgccgg aactcgatgc ccgcggcagc gatgaaatcg ccaggctcgc
     1441 ggcctcgttc acgcgcatgc gccgcagcct cgaaaacgcg atgcggctgc tgggcgaatg
     1501 acgcgaatcc cgcctcgaaa gagggatact gccgatgtca gccgccgccg gatacgaagc
     1561 ttatgacgga acctgcctca ccccgctgcc ggcggcggac ctgcctgccc cgccgccccg
     1621 tcgcacgcgc cggcgtccgc tcgtcggagg cacgaccgtg agcacgatgc ccgtcaccat
     1681 cggcaagtac cgcatcgagt cggtgctcgg gcatggtgcg atgggggtcg tctaccgcgg
     1741 ccacgatccg gtgctcgatc gcacggtcgc gatcaagacc atccgtgccg aactgctcgc
     1801 cgccggcgac gacggcttcg aggacgatga ctggcacgcg cgcttccgcc acgaggcgcg
     1861 cgccgccgcg cgctgcacgc acccgaacat cgtcacgatc tacgacttcg gcgagacgcc
     1921 ggacggttcg ccgtatctgg tcatggagtt cgtcgagggc cgcgagctgc gccgccacct
     1981 cggcgagggc cgccagttcg acctgccggc ggcggcgctg atcctgcgcc agatcctgcg
     2041 cgcgctcgac tgcgcgcacc tgctcggcat cgtgcatcgc gacatcaagc cggccaacgt
     2101 catcctgctc gccgacggca tggtcaaggt caccgacttc ggcatcgcgc gcctgagcgg
     2161 cggcaccggc ctgacccaga ccggcgcgat ggtcggcacg ccgaactaca tgtcgcccga
     2221 gcagttcacc ggcggcacgc tcgaccggcg cgccgatctc tacgcctgcg cggtgatcgc
     2281 ctacgagctg ctgaccggcc agcggccgta tccgggtccg acgccgccgg cgctgctgca
     2341 ccagatcctg aacacgacgc cgccgcgccc gagcgcgctg cgccccagcc tgccggcggc
     2401 gctcgatgcc gtgctgctgc gcggcctggc ccgcgacccg ggcgcacgct ttgccgatgc
     2461 ctgcgaattc cagcgcgcgc tcgatgccgc gctcgccccg gccctcgcgc cggtgccgat
     2521 ccaccaccat ccggagcccg gcaccgtcgt gatgccggca ccgctgacgc tcagcgacgc
     2581 cgagctgcac gaggccgaaa ccacgctggc gacgcacatc ggtccgctcg cgcgcgtgct
     2641 ggtgcgccgc gccgccgcct cggcgcactc gcgcgatgcc tggctcgaag agatcgcccg
     2701 caacatcagc gacgatgacg agcgccggcg cttcctgcgc cggctgcgcc gcggcgaacc
     2761 gaccggctac acgcagtacg gcaccaccgc gcataccgcc cacaccggtc acgtcggcca
     2821 cgccggcggc aacgccgcac cgaccgccgc ttccggctac ggcagcagcg gctcggtgac
     2881 gacggtgctg cgcgtcgacg cggccacact cgccaccgcg cagcaggcgc tgacgcaggc
     2941 gatcgggccg atcgcgcgcg tgctggtgcg ccagacggca ccgctggcgc aggacgccgc
     3001 cgacctgtac cggcgcctcg ccgagcgcat cgacgacgcc gacgcgcgcc ggcgcttcct
     3061 cgccacccgc ccgcactgag accggcgccc gggcgggcgg gcgacgcgca tgtccgcggc
     3121 gcatccgtcc cgcgtgcctt cgcgccccat ctgccgcgtc cctcccgctt tcttccgctt
     3181 cctcccccgg ttcatccgcc gtcgcagggc cgcagcgcct gcatgtccgg catgcagcga
     3241 tcggcgcacg ggcgtgctgt tgcagcgcag tacactgccc ggcaacccgc ccctgcccac
     3301 ggactgtact gctgcgatgt cctcctcccc cgctcctgcc gcagtcctcg ccgagccgcc
     3361 gctgcgctcg ctggcggcgt tccggcactt ctggctgacg cgcgtctgca ccacgattgc
     3421 ctatcagatg atcggcgtcg cggtcggctg gcgcgtctac gagctgaccg gcagcgcgct
     3481 ggcgctcggg ctgatcgggc tcgcgcagtt cctgccggcg ctgctgctgg tgctgccggc
     3541 cgggcagatc gccgaccgtc acgaccggcg gcaggtcgcg gcccgggcgc agcgcgtgtc
     3601 ggcgctcgcc gcggcgctgc tcgcgctcgg cagcctcggc gactggctga cggtgcacgg
     3661 catctatgcg ctggtgctcg tgatcggcgc ggcgcgcgcc ttcgaggcgc cgtcgctgca
     3721 ggcgctgctg ccgcggctgg tgccgcccgc ccggctcggc agcgcggtcg cggcctcggc
     3781 ctcggcgatg cagaccgccg tgatcaccgg cccggcgctg gccggtttcg tctacgccgc
     3841 cgggccggca gcggtgttcg cggccagcgc gctgctgtgg acgctcgcga gcctgcaact
     3901 ggcgcgcatc gagccgctga accgcccggc caccagcgcg ccgccgccgg ggctcgacac
     3961 gctgttcgcc ggcatcacct tcatccgcag ccggccggtc gtgctcggcg cgatctcgct
     4021 cgacctgttc gcggtgctgc tcggcggcgc gaccgcgctg ctgccgatct acgcgcgtga
     4081 catcctgctg accggcccgc tcggcctcgg cctgctgcgc tcggcaccgg cgatcggcgc
     4141 gctggcgatg tcgctttggc tgtcgcggca tccgccgcag cgcggcgtcg gccggctgat
     4201 gttcgcggcc gtcgcggtgt tcggcgtcgc aacgctggtg ttcgcgctgt cgacgcagtt
     4261 cgcgctgtcg ctggcggcgc tggtcgtgct cggggcctcg gacatgatca gcgtcgtgat
     4321 ccgcgcctcg ctggtgcagc tcgaaacccc ggacgagatg cgcggccgcg tcagcgcggt
     4381 caattcgatc ttcatcggcg cctcgaacca gttcggcgaa ttcgaatccg gactgaccgc
     4441 ggcgctgttc ggcacggtgc cggccgccgc gctcggcgga ctcggcacgc tgctcgtggt
     4501 gctggtgtgg atgcggctgt tcccggacct gcgccggcgc gagcgcctgc tggaaaccgg
     4561 caccggctga aagcccccgg cggccccgct gtccgaaaat taacctctgc gacggaatgg
     4621 ctctgccata tcgggtacgc cgtccgccgg gccccggtgg ccggacggcg gcactccgtg
     4681 catctccccc cgatccggcc attcccccgc caggaggttt cccatgcatt tcagcaagcg
     4741 actcggtgcc gagttcatcg gcacgttctg gctcgtgctc ggcggttgcg gcagtgccgt
     4801 gctggccgcg gcgtttccgc aggtcggcat cggcctgcac ggcgtggcgc tcgccttcgg
     4861 cctgaccgtg ctgacgatgg cctacgcgat cggccatgtt tccggctgcc acctgaaccc
     4921 ggcggtcacg gtcggcctga ccgtcggcgg ccgcttcccg gcgcaggagg tcgtgcctta
     4981 tgtcgtcgcg caggtgctcg gggcgacggc agcggccttc gtgctgtatc tgatcgccac
     5041 cggcaaggcc ggcgcgtcga tcggcggctt cgcggccaac ggctacggcg agctgtcgcc
     5101 cggcaagtac ggcctcggcg cggcgctgat caccgaggtg gtcatgaccg cgatgttcct
     5161 gttcgtgatc atgggcgcga ccgatgcacg cgcaccggcc ggcttcgcgc cgatcgcgat
     5221 cggcctgtgc ctgacgctga tccacctgat cagcatcccg gtcaccaaca cctcggtgaa
     5281 cccggcgcgc tcgacctcgc aggccctgtt cgccggcagt gaatacctcg gtcagctctg
     5341 gctgttctgg ctcgcgccga tcgtcggcgg cgccatcggc gcactcgtct accgcgcggt
     5401 gtgcagcgag ctgaagccgg ccgtggcacc ggtcgaggat ctgcgcccgg ccgcctgagc
     5461 cggcatctgc ccctgccctg cccccgggcg cccgcgcggc gcccggggcg tctgcggcgg
     5521 ctggcatccg cggcggcgct ctggaaggat gcgcgacccg tcccgcccgg agtatctgcc
     5581 ccgatgtccg atgcccgcct gcgcgccacg ctgaccggcg ccgtcgcgat cctgctgtgg
     5641 gcgacgctcg cgctcggcgc gacgctctcc gccggcctgc cgccgttcga gctgctggcg
     5701 ctgaccttct cgatcggcgg cctcgccggc ctcgccgtcg tcgcgcgcgg cggccggctc
     5761 gcgcggctgc gccagccgcc ggcggcgttc gcgctcggcg tcgccgggct gttcggctat
     5821 cacttcttct atttcgtcgc actcgcgcac gcgccgccgg tcgacgccag cctgatcaac
     5881 tacctgtggc cgctgctgat cgtgctgttc tcgggcctgc tgcccggcga gcgcctgcag
     5941 cgccaccacc tcggcggcgg cctgctcggc ctcgccgggg cctgggtgct gatcagcggc
     6001 ggcggcacgg cgccgaggct cgaatacctg ccgggctatg tgtcggcgct ggcctgcgcc
     6061 ttcgtgtggt cgggctacac ggtgctgaac cgccgcttcg ccggcctgcc gaccgatggc
     6121 gtcgccggcc agtgcctcgc ggtcgcggtg ctggcggcgc tctgccacgc ggcctgggag
     6181 cccacggtcc ggccctcggc cgtgcagtgg gcggtgatcg gctggctcgg actcgggccg
     6241 ctcggcttcg cgttctacgt ctgggatcac ggcagcaagc acggcgacct gcagctgctc
     6301 ggcgtgctga gctacgcgac tccggtgctg tcgacgctgc tgctgctcgc ggccgggcgc
     6361 ggcgagccgg gctggccggt ggcgctcgcc tgcgcgctga tcgtcggcgg tgcgctgctg
     6421 gcctcggggc ggttccggcg cggctgaagc cgcaaatgcc gcggggcgcg tgcccgggat
     6481 gacacgcgcc ccgcaaaaac cccgaagccg gcggcgctta ctcgcgcacg ccctcgatga
     6541 cgagcttcag gttcaccgca tcggccgccg ggccgaggtt ctcggtgtag ccgaagtcgc
     6601 tgcgcttgat cgtcgtcgtg ccgacgtagc cgcggcggta gccgccccac gggtcattgc
     6661 cggcgccgat gaactgcacg tcgaccttca ccggcttcgt gacgccgtgc agggtcagct
     6721 cgccttcgag ggtcgccttg tcgccgctct cgctgaactt cgtgctcttg aagctcgcgc
     6781 tcgggaactt gtcggtgaac aggtacttct cgcgcaggtg cttgtcgcgc tcgccgtagt
     6841 tggaatcgac gctgccggtg tcgaccctga cctcgatggt cgaggcgccc ggcttcgcgg
     6901 cgtcgtacgt gaacttgcct tccatcttgt tgaagcggcc ctgcagcatc gagaagccga
     6961 ggtgcgagat ctcgaagttc acgaacgagt gcgccggatc gaccttgaag gtctcggccg
     7021 cggccgtgcc ggcgaatccg agcgacagcg aggccgcgag cgcggcggcg agcagagggg
     7081 acttgcgcat ggtgagcagc tcctgagggg cgacggcggc atcggccatc gcgatcgggg
     7141 gaggaaaccg ccgcccgcag cgggcggcgc gaggaaaacc gggaatccgg aaccacgcgg
     7201 ccccggaata cttcagcgcg gcagccggcc ggtcatgcgc gccagcgtgc cgtcgcggtc
     7261 gaggaactgg tgcttgagcg ccgccgcggc atggacgacg acgacggcga gcgtgcccca
     7321 ggcgagcgtc tcgtgcaggc cggcgacgaa ctcctgccac tgctcgccgc cgctccagag
     7381 cgccggcagc gagatcagcc cgaacagctc ggccggatcg cccttggcgg tcgagaccag
     7441 atagccgctg agcgggatca gcagcgtcgc cagatacagc aggccgtgac cggcgtgcgc
     7501 gccgagcttt tcccagggct tcagcgtcgc tgcgagcggc ggcgcgcgat ccgcgatcgc
     7561 ccagccgatg cgcacgagcg ccagcaggcc gaccacgacg ccgaagccgc ggtgcaggcc
     7621 cggccacagc cggtagccgg ggtcgtagta atcgagcgtc gtgatgtacc agccgagcgg
     7681 gatcaggccg aggatcagca cggcgatgct ccagtgcagc aggcgggccg tcaggccgta
     7741 ggcggatgcc gaattgcgcc acatcgcgga ttgacctcgc gcaagcgggg gacaccggca
     7801 gcggtgtcgt tcgagttgcg cagcaggcta gcagccgcat tccggcacag cgacgctgct
     7861 tgcgtgacag gattgttcgc tctggattga caataccgcc ggaacttttg cggacctgcc
     7921 ctttttgcgc gcacgacggc gcgccggcaa agtcacgaaa aacacaaaaa acggcggccc
     7981 ggcgccgcaa cgccgggccg ccgtgtcgag cggccgagat gcaggccggc tcaggtgcgg
     8041 cgcgtcgcgc cgacgaccac cagcgcgccg ccgagcgcca gcgcggtata gccggcccag
     8101 gccgggatgc cgacgtgctt ctcggtctcg gccgtggcct tgaccggtcc gacctgcagc
     8161 acggtctcct cgctggtgta gttgaagccg cggtaggcga gcacggcgat gccggcgacc
     8221 agcagcagca cgccgatgat catggaagtc ttcatggcgg atacccctga aaggctgcgg
     8281 tgaaaatgca gtgaaggcgc agtggagagc ggtgtcaccg tcgcacggtc gacctctgcg
     8341 ggccgtcccc tgcacgcctt cggcgcgtga cggtcacacc ggacatcgac ccctgcgcca
     8401 caatcagggt tccctgcagg actgcatccg ggattgccgg cggtcatgcc ttacggtttg
     8461 cgacacgcgc agcgcgccgg cacggcaaac tgtgcagacc gggtgcagac cgatccggat
     8521 cgaatcccct ccagccatcg tgcgaccgca ctgcggttgc cgcacccgcc gcaccgggga
     8581 aaggaaggtc agatgagcga agaaatcagc cgcgactacg tacacggcgt ctacaccgaa
     8641 ctgctcgccc gcgaaggctt ccggccgaag accgacgagg acggcgacat ccagttccgc
     8701 aaccagggct tcacgaccta catccgcatc agcgaagacg atctgccgtt cctgcagatg
     8761 ttcatcccgt cggtgtacac gctggagtcc ggcgaggagg acacggtcgc gctggcggtg
     8821 atggccgagc tgaaccgcca gttcaagctc gtgaaggtct gccggctcga tcactcggtc
     8881 gcggtgctgg tcgagatgct gctcgcgcag cccgaggacc tcggcggctc gctgctgcgc
     8941 atcgccgacg tgctggtggt ggtcgcgcac gaattccacc ggctgatgag cgcggcgctg
     9001 cgcgagcgcc agcgtcaggc cgagtccgag ctcgacgagg acgatctgcc gatgccgacg
     9061 cgcaccgtca actgacggac gagccgcgtc ctcagccgcg acgcggctcc gcggcagggc
     9121 cggaaccggg gccgaggccg atgccgtact ggcgcagctt gttggcgacc gcggtatgcg
     9181 acaggccgag gcggcgcgcg agctggcgcg tgctcggatg gctcggatac aggcgcgcga
     9241 gcatcagccg ctcgacgcgc gccagcgcct cggcgagcga gccctcgaac agcgcctcgt
     9301 cgatcggcgc cccgctcgcc gccgtggcgt cggtgccgag cgcgagcagc tcgggcgtga
     9361 tcgcgccgcc ctcggcgacg gtcacggcgc gcagcaaggt gttttcgagc tggcggacgt
     9421 tgccgggcca gccgtgcgcg agcagcgccg cctcggcctc cggcgtcagc cgcggcggcg
     9481 cgcaggcgag ctggcgcgcg gccatctcga tggcttcgag cgcgaccggc agcacgtcgg
     9541 cctgccgctc gcgcagcgac ggcatctgca ggttcagcac gtgcaggtgg tagtagagat
     9601 cgtcgcgaaa gcgtccgtcg cggacctcgg ccgccagatc gacgcgcgtc gaggcgatga
     9661 tgcgcacgtc ggcgcggcgc ggctcgacgc tgccgccgcg ccggaagcgt ccgtcctgca
     9721 ggaagccgag caaccgcgcc tgcagcgccg gcgacagctc ggcgacatcg gacaggaaca
     9781 gcgtgccgcc ggcgccctgc tcgaacaggc ccggctgctc gcgcacgccg aacagcacgg
     9841 cctcggcgcc ggcatcggcg agttcgccgg cgttgaggcg caggaacggc gcctcgcgcc
     9901 gcgggctggc gaggtgacac gcgtaggcga gccgcgcctt gccggagccg gattcgccga
     9961 ggatcaggat cggcgccgac agcggcgcca tgcgccgggc ctgcgccacc agcgcacgca
    10021 tcggcgccga gcggtgcacg atcgacgcga agccggcatc gacggccggc gaggcggctt
    10081 cgaggctgcc gccgtgcagc accatgaccg cccccgacag cgacttgccg gcgcgtgcga
    10141 gcggcaccag caccggaatg aagtcggcaa gcacggcctg gccgcgcagc gccagacgca
    10201 cgccgtactc gggccgatcg agccggttga tgtgctcgac cagatcgaag tcctcgacca
    10261 gcggcgcgag cggcgtgccc tgaccgtccg gccccttgag cttcagcagg cgctgcgccg
    10321 attcgttggc gacgacgacc cggccttcga gatcgagcga cagcaccggc gccggcaggg
    10381 cgccgagcag cgtgcgcagc tccgactgct tgcgctcgcc cggcatcagc gggatcggcc
    10441 gcacgtcgcg cacgcccggc acctggcgga tctcgggcag cacgcggtgc agctcatcga
    10501 gcgtcatctg cgcggcgtgc agatagaacg aaccgcggcc gtcgttctgc gtgacgatgt
    10561 cgccggcggt catgttgatg ttgtgccgcg ccagactgtt cagcacggcc atcgcgatgc
    10621 cgacgcggtc atcgaacacg atctgcaggc gcatggcgtc tccttccggg attcaggtca
    10681 tccggcatcg cacggccgcc tcggcaacgc acgacaccgc agggtcgtat ggctgtgtgt
    10741 acgtccgcag cagattagtt gacgccgggc gccgatcaca acgtcagact gcatgcgggt
    10801 tttcgtcgat tcctttctta cctgtaaccg atcattgaca atcccggagc tcgccggacc
    10861 ctgcccgcag ccgtggccgc cacggcggtg cagcagcgcg cacccggcct ctgctacctg
    10921 tgcgccatcc ccgtccggcc tccgtgccgg cgatcgcgcc cggaggccac gccgcgccat
    10981 gaacatcgcc ctgtccctgc tgcacgcgct gaaggcgcac ggcgcccgcg agatcttcgg
    11041 cattcccggc gacttcgcgc tgccgttctt ccgcatcgtc gaggaaagcg gcgtgctgcc
    11101 gctgtacacg ctgtcgcacg aacccggcat cggcttcgcg gccgacgcgg cggcgcgctt
    11161 caacggcgca ctcggtgtcg ccgccgtcac ctacggtgcc ggggcgctga acatggtcaa
    11221 cccggtcgcc ggtgcctatg ccgagcgcag cccgctggtg gtgatttccg gggcaccggg
    11281 cgcggccgag gtgcgctccg gcatgctgct gcaccatcag gtgaagacga cggactcgca
    11341 gttcgcgatc taccgcgaga tcacctgcgc gcagacccgg ctcgacgatc cggcgacggc
    11401 cccggccgag atcgcgcgcg tgctcggcgc ctgtcgcgag cagtcgcggc cggtgctgat
    11461 cgaggtgccg cgcgatgcgg tggcgctgcc gtgtgcgccg gtgcgcccgg ccgcgccggc
    11521 cgcctacgac cgcgaggcgg tcgaggcctg cgccggggaa atcctcgcgc gcctggccgc
    11581 ggccgcccgc ccgacgctgc tcgtcgacat cgaggtgcgc cgctacgggc tggaggcgcg
    11641 tgtcgccgaa ctcgcgcacc ggctggcgat tccggtcgtg acgaccttca tgggccgcgg
    11701 cctgctcgcc gaggccgcgg tgccggtcgc cggcacgtat ctcggggtcg ccggcgagcc
    11761 ggcggtgacg gcgctggtcg aggaatcgga ctgcctgctg atgctcggtg tgctgctgtc
    11821 ggacaccaac ttcggcgtct ccggccgcca gatcgacctg cgcaaggccg tgtacgcggc
    11881 cgaccggcag gtgcagatcg gcttccacca ctatccggac ctgccgctcg gcgcgctggt
    11941 cgaggcgctg ctggcgctgg tcagcgacct cgatccgccc gagccgcgcc tgcggcccga
    12001 tctgccgccg ctgcccgagg cgatcgtcga cggcgcaccg atcgcgcccg atgacatcgc
    12061 cgcggcgatc ggcacgctga tgcgccgcca cggcgcgctg ccgatcgcct ccgacatcgg
    12121 cgactgcctg ttcacggcgc tcgggctcgc caacacggcg ctgaccgccc ccggctacta
    12181 cgcgacgatg ggcttcggcg tgccggccgg gctcggcatc caggcgacca ccggcctgcg
    12241 gccgctgatc ctggtcggcg acggcgcctt ccagatgacc ggctgggaac tcggcaactg
    12301 ccggcgctac ggctgggacc cgatcgtcat cgtcttcaac aacgccagct gggagatgct
    12361 gcacgtgttc cagccggaat cggcgttcaa ccgcctcgac gaatggcgct tcgccgaact
    12421 cgccgccgcc ctcggcggcc acggcgagcg cgtgcgcacg cgccgcgagc tgcacgccgc
    12481 gctcgcggct gcgcacgccc gccgcggccg cttctcgctg atcgaggcga tgatcgcgcc
    12541 gggggcgctg tcgcacacgc tggcgcgcta cgtcgagggc ttccgggccc ggcgcgcgcg
    12601 ccaggcggcg ggccactgag cgaccgcgcc gcgaaacaga ccgctgttca cgctcgcacc
    12661 gtctacgctg atccggtcct gacggagccg gacccgcaag ggtccggttt caccctgtcg
    12721 ccccgtctcc cgcctccgga gccggggcgt ctttttgaga acgccggggg tccgttgccg
    12781 gcaagtccgc gattccggcg tggttgacgc agtgcgacag gcttgcgtag ggtgcgcgcc
    12841 catccgctgg aaacgccccg ggtggtccga gcgatcggac tgaaacggga agccggtgca
    12901 acaccggcgc tgcccccgca actgtaagca cgccaagcga cgcccccgat gccactgtgc
    12961 ccgaccggca cgggaaggcg ggcgtcgccg ggagaggaca ctccccctcc cgcgtgcgag
    13021 ccaggagacc ggcccggggc gtacccagtc ggtgttgcgg cgggcgacac cgacaagccg
    13081 ctccggaagg aactgccgat gcgcaggccg gggcgtgcct gtcctcgccg cggcgaccgg
    13141 cctgctgccg gcgccgcggg cgtttcgccc ccattccgtt cctgcgaacc gatggggata
    13201 ccctcgatga acaccctgca caccggcctc gccggtctgg ccgcgctcgc ggccctgtgc
    13261 cctgcgcccg cagcggccgc cgacggcgac agctccgaac tcgtggtcac gatcaccggc
    13321 gcgcgcagcg agcagccgct cgcgcatgcc accgccccga tcacggtcat cgaccgtacc
    13381 gaactcgacc agagccaggc gatgacgctg aacgaggccc tcgccgggcg caacggcatc
    13441 gccgccgcca gcaacggcgg cgccggcaag gtcaccaatc tctatctgcg cggcgccaac
    13501 gccggccaga cgctgctgct ggtcgacggc atccgcctcg gctcctcgac gctcggctcg
    13561 ccggcctggc agctgctgcc gctcgggctc gccgagcgca tcgaggtcgt gcgcggcgcg
    13621 cagtccggcc tgtacggctc ggatgcgatc ggtggtgtcg tgcaggtgct gacgctgccg
    13681 gcgcccggca gcccggtgcg gccctgggcc gaagccggtc tcggcacgca gcagggacgg
    13741 catctcggcg ccggcgtgcg cggcagtcag ggcgcgacga ccttctcgct cggcggcgac
    13801 tggctcagca gcgacggctt cgatgccacc gccaccgaca ccttcgcgca caacgacgac
    13861 cgcgatggct tccgcagcgg tgccggccac gtcgccgtgc gtcaggacgt ggcgccgggc
    13921 cagtccttca gcttcacggc gctgcgcgcg cagggcgaga accgctacga cggcagcaac
    13981 ccgctcacct tcgcgaccgc cgatcacgcc gaaggcaagt tcgtgcagca ggtgctggcg
    14041 ctcaatgccg acaaccggct gacggatgcc tggcgcatga agaccacgct cggccagagc
    14101 cgcgaccaga acgaggactt cgccgatggg ctctcggccg gcgccttcga cacgcgccgc
    14161 gaccagttca cgtggcagca cgagttccgg ctcgccgacg gccacctgct gagcctcggc
    14221 gccgagtggc tgcacgagaa ggtcggcggc agcggcgtcg tcgattacgc gcagaagacg
    14281 cgcacgacgc gcgcgctgtt cctcgtcgat gccgtgacgc tcggcgctca cgacctgcag
    14341 ctcagcctgc gcagcgacga catcgacgac atcggccgcc acaacaccgg ccgcctcggc
    14401 tggggctgga gcttcgcgcc cggcctgcgc gccacggcca gctacgcgac cggcttcaag
    14461 gcgccgacct tcaatgatct ctactacccg gccggcccgt tcgccgccgg caacccggac
    14521 ctcgaacccg agtcctcgca cggctgggac ctcggcctgg agcagcacgc cggcgcctgg
    14581 cactggcggc tcggcggcta ccgcacccgc gtcggcaacc tgatccagtg ggtcccggac
    14641 gagttcttcc gcttcagtcc ggtcaacgtc gccaatgccc ggctgaccgg catcgaggcc
    14701 tcgctcggct ggcaggatca cggctggcag gtaaaccttg atgccgactg gctgcgcgcc
    14761 gaggacaccg acagcggcga cccgctgccg aaccgcccga accgcagcgc ccgcctcagc
    14821 atcgactacg cccccggcac ctggagcgcc ggcgccacgc tgctcgccgc cgcctatccg
    14881 gatgacctgc cgcagcgcgg ctggagcgtg ctcgacctgc gctccggcta ccggctcgac
    14941 aaggacttcg agctgaagct caaggtcaac aacgtcttcg accgcgacta ccagaccgtc
    15001 aacggctatc cgatgccggg gcgcgaggcc tggatcgcga ttgaatacgg catgcgctag
    15061 aacagcgcag tcgacgcacg cctcgcccgg ccgtgatctg ccgcgctccc ccgcccccgg
    15121 caagctgccg cgtgcagccc cttccccctc gcggggaagg ggtcggggaa agggggagag
    15181 ccgccggcac cagcgccgaa accatgcgcc gcgcgccatc ccttgcgggg ggcagcgcgg
    15241 cgcggcggcc acggccggcg gagctcctgc cggccgtggc cgatccatct cccgcccgag
    15301 gactccccgc gatgggtcac cgcctctcga agatctacac ccgcaccggc gatgccggca
    15361 ccaccggcct cggcgacggc acgcgcgtgc ccaaggacgc gctgcgcatc gaggtcatgg
    15421 gcgaatgcga cgaactgaac gccgtgctcg gcatggtgct ggcgctgccg gtgcgcgagc
    15481 cgatccgcgc cgtgctgacc gaggtgcagc accggctgtt cgacctcggc ggcgagctgt
    15541 gcatgcccgg ctacgcatcg ctgagcgccg aggacgtgac gcagctcgaa accgatctcg
    15601 acggcttcaa cgccgcactg ccggcgctga aggaattcat cctgcccggc ggcggcccgg
    15661 cggccgcggc ctgccatctg gcgcgcaccg tctgccggcg cgtcgaacgg catctggtga
    15721 cgctggtgcg caccgaggcc gtcaacccgc acgcgccggc ctacctgaac cggctgtcgg
    15781 acctgctgtt cgtgctcggg cgcgtgctcg cacgcgacga aggcggcagc gaggtgctct
    15841 ggcgcaacga ccacggccgg cggcgcacgg cggcggacca gccatgagct tcaccgaaaa
    15901 cctcgccagc ctgccgccgg ccgggcatct ggccgcgctc gaactgttcg agcacggcga
    15961 gccggccggg cgcatcgaga accgccccgg ccaggccggg tcgctgcgca tctaccacgc
    16021 ggtcgcggtc aacggccgca tcgacgccga agcggcacgc cgggcgctcg ggctctacgg
    16081 cgaacacctc gccgatgccg aggccaatcc cggccggcat ccgaacatcg accggctgct
    16141 ggcgctgatc aggcgcaaca gcgtgctggt gatcaggccc gtcccggccg cggacggcgc
    16201 ctgattccgg ccggtcacgg cgcaccggcg gccccgctca cggcagcgca tccaccagca
    16261 cgcccgcgaa caccgcgagt cccacccagt tgttgtgcag gaaggcgtgg aagcatgcgg
    16321 ccggctcgcg tctgcgcacc agatactgtt cccaggccat cagccaggcc gcggcgaaca
    16381 ggccgaggta gtagaccggc ccgcagccgg ccatgagtcc ggccagcccc agcagcagca
    16441 ggaacgcgcc ctgcagcagc gcgacgatct gccggtcgcg ggcgccgaac aggatggcgc
    16501 tcgacttgac gccgatcttc aggtcgtcct cgcggtcggt catcgcgtag aaggtgtcgt
    16561 aggccaccga ccagacgatg ttggccgcat acagcagcca cgcgagcgcc ggcaccgagc
    16621 cggtcacggc cgcgaaggcc atcgggatgc cccagccgaa ggccatgccg agcacgacct
    16681 gcggcaggtg atgcaggcgc ttcatcagcg gatagccggc ggcgagcgcg agcccggcga
    16741 aggacaggcc gatggtcagg ccgttctgcg tcagcaccag cccgaaggcg acgaggctca
    16801 ggccgatcgc gagccacagc gcctcacgca ccgtcacgcg gccggcggcg agcggccggt
    16861 cgcgggtgcg cgcgacgtgc gggtcgaagc ggcggtcggc gatgtcgttc atcacgcagc
    16921 cggccgagcg catcaggaac acgccgaggc agaacaccag caccgtgccc tcgggcggct
    16981 ggccgtgacc ggcgagccac agcgcccaca gcgtcggcca cagcagcagg aagctgccga
    17041 tcggccggtg catgcgcgca agctgcgcgt agtcaccggc cttggcgagc agccgcggcc
    17101 cgcacagggc ggcgcggggt ttgccggagg cgggggcggc gggagcgggt tccggactcg
    17161 gtttcatggg aggcgatcac gcttgcagga cgtcggcgag cgccggcagg aagacttcgc
    17221 agaccagcag cggccggccg tcgatccgga aggtcgagcg ccggccccac agcggcaggc
    17281 cgctggcacc ccgcacgtcg ag
//
LOCUS       DAOQIE010000035        17117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1550_length_17117_cov_7.087856, whole genome shotgun sequence.
ACCESSION   DAOQIE010000035 DAOQIE010000000
VERSION     DAOQIE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 17117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17117
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_17117_cov_7.087856"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK144_03795"
     CDS             complement(<1..268)
                     /locus_tag="PK144_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF64880.1"
                     /translation="MSTSDTPALPANAAAAIIPPDLSALPPTALLDTASTAAALGLKP
                     ETLANWRTYGRYDLPYIRSGRLIRYRVADVLGFIERRTRLHTGQT"
     gene            complement(414..1169)
                     /locus_tag="PK144_03800"
     CDS             complement(414..1169)
                     /locus_tag="PK144_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64881.1"
                     /translation="MLLDDFKREFECKINGLSKNELRLVANALLSDRKKIYAKGVDVS
                     DIISEGLARELFANISRGSDPFLESYQSKTFLDRIIVLFKWINENTDNGLSDSCSTSN
                     TKATQQESNDIKAMLHDAQTQGRKAIEAAAAAETRAAAAERERDDLRERQTETEQQTA
                     HDAPNVRSAVQIARHATKKAETLAAALYLLAANPSDYRDGTGRVIAARLAQAVNDMAH
                     RWWPDGATPLSIKTMSEHIARALTDSRVIDEDS"
     gene            complement(1419..1859)
                     /locus_tag="PK144_03805"
     CDS             complement(1419..1859)
                     /locus_tag="PK144_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNF64882.1"
                     /translation="MSTDLYDTDLYAWATGNAALIRAGHLAEVDFEHIAEELESMGAS
                     ERNALNSRMTELLLHLLKWRFQPERRGASWEVSIIKQRNGIADLLDASPSLRARLPEV
                     LAKTWPRALRLAVAETRLPAATFPEDCLFALDDVLAAEFWPDPA"
     gene            complement(1870..3156)
                     /locus_tag="PK144_03810"
     CDS             complement(1870..3156)
                     /locus_tag="PK144_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HNF64883.1"
                     /translation="MPTTRIKFTKRALEAVPAAAAGKRETLLDTECPALAIRVTDKGV
                     KSFILQRRVNGKPTFVTLGRFPEMTVEQARKAATGAHVQINDGTDPNAVKRADRARKL
                     TLADVLDDYLKGRDLKPRTVAGYRRQVEVNLAEWKDKPLLSISRDMVERKHASLSEPR
                     SYVVNGRSVRRGSEAEANYAMRVLRALMTYAAGKYEDEDGAPILSDIPTRRLSATRAW
                     HRIERRQTVIKPTQLADLHAALWRVQPVRGPRYDDDARAYLELLLLTGLRKNEAAQLR
                     VADVDLAARTLTVPDTKNRRPHTLPLSDRLETLLRSRIEAAGTDPATLLFPHVRDVRR
                     LIERIATETGITFCPHDLRRTFITIAESLDIPAYALKRLLNHKSGADVTAGYIVIDTE
                     RLREPMQRITDFMLKAAGVRESAAVVPITRNAGTAG"
     gene            complement(3163..>3342)
                     /locus_tag="PK144_03815"
     CDS             complement(3163..>3342)
                     /locus_tag="PK144_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64884.1"
                     /translation="VACYRPSDFESDFRPVTHHHPSSLIVDISVTWAHASITGHHSKP
                     PKPYARRMPGVCPTW"
     assembly_gap    3343..3442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3630..3788
                     /locus_tag="PK144_03820"
     CDS             3630..3788
                     /locus_tag="PK144_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNF64885.1"
                     /translation="MSLGTILLIVLILLLIGAIPAWPHSRDWGYYPSGGLGLVVVVVI
                     ILVLMGRI"
     gene            4248..7046
                     /gene="ppc"
                     /locus_tag="PK144_03825"
     CDS             4248..7046
                     /gene="ppc"
                     /locus_tag="PK144_03825"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNF64886.1"
                     /translation="MPVTDDKGLRARVKLFGNLLGEVLRTQESGQVFDAVEALRKGFI
                     ELRTSEDPARRAQLMALIEALDPQTLTHVLRAFSSYFLLANIVEEDWAHQERRHRVAS
                     GEPLWRGSFDDTVRELVAAGVSAEQMQGLLDSLLFQPVFTAHPTEAKRRALLEAQRRI
                     FVMAKKLNDAGLGGYAREAVERQLLNQIQILWKTDEVRVHKPQVRDEIVNGLYYFRET
                     LFHALPHIYRNLERALATNFGEAAAAIRVPSFIRYGSWIGGDRDGNPFVTHVVTALAV
                     RLQARTVLREYVQRLEDLSHLLTHSSSLVRPSLAFAERLAADAPLVPEVYADEPHRFS
                     QEPYRRKLFLMRARLLRNLDRLNAAIDGHEAPVAAGYASERDFLEDLYAIRDSLHSHG
                     DGNLADAELKDLIRLAESFGFYLAQLDVRQESTRHTDAVTELFARAPNLPDYAALSED
                     ERLQVLGELLAHPGTPLLYAEDLSENTREILAVLRTVASLRDEISPNAFGAYVISMTH
                     AASHVMEVLFLASFAGLAGRRGDGSWFCDLRVSPLFETIADLAHIEPVLERLLDNPTY
                     RELLAVSGNVQEVMLGYSDSCKDGGILASAWNLYRAQKKIVTITARRDIRCKLFHGRG
                     GTVGRGGGPTHDAILAQPAGTVQGQIKFTEQGEVLSSKYSNPETAVYELTMAITGLIK
                     ASRCAVTECIPDEPQFLTVMEQLAKDGEQAYRQLTDHTPRFIDYFYEATPVTEIGLLN
                     IGSRPSHRKKADRSKYSVRAIPWVFGWGLSRQIIPGWYGLGAALSAYRSGDSGRLDTL
                     RRMYAEWPFFRSLLANAQMSLGKSEMGIAHEYAQLCLESGTADDIYGTIRSEHDETVA
                     QVLAVAGIEEILDENPRLKLSLARRDPYLDPINHIQICLLRRYRQAEGGGEAERALWL
                     NPLLRSINALAAGMRNTG"
     gene            complement(7095..7862)
                     /locus_tag="PK144_03830"
     CDS             complement(7095..7862)
                     /locus_tag="PK144_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNF64887.1"
                     /translation="MGWLRDWRRRRVLRQSEADAPTWQAACDAQAVLAGLPQADQQRL
                     RRLATLFLHEKTFEAAAGLTLTPPMRWRIAAVACLPVLNLGLDWYEGWSAIVVYPGEF
                     MARQDFTDEAGVVHQLRRPLIGEAWERGPVILSWADVLDSETATDGFNVVIHELAHKL
                     DLLNGEANGLPPLHRGMSVRAWADIFTRAYDDFCAHVDAGEDTAIDPYASDSPAEFFA
                     VLSEAFFETPAVLNATYPPVYAQLRAFYRQDPLLRLA"
     gene            complement(7869..8111)
                     /locus_tag="PK144_03835"
     CDS             complement(7869..8111)
                     /locus_tag="PK144_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64888.1"
                     /translation="MNSRERQALVGALLLTALMTAALVWLVNEGWQRLPAWLAVILCL
                     LLAVFVIGSPIWALARWSERWLARQQRDRQTQQRRD"
     gene            complement(8098..9189)
                     /locus_tag="PK144_03840"
     CDS             complement(8098..9189)
                     /locus_tag="PK144_03840"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNF64889.1"
                     /translation="MPIPLAASLSRRRAQTLRSLLEVPGPPLIIGVAGDSSAGKTTYT
                     NGLRRMLGDDIVGTIEMDGYHKEDRATRARTGRSPLDPEMNHLGLLREHLEALRRGES
                     VHLRRYDHGTGTFIAPQPFSPKPILVLEGLHALYPEFTSLLDYTVFVDPARDIKLGWK
                     IARDTQFRGHRVEPLADEIRKREAAFRRWIEFQKTDANVVIKINRSKLKLLANEEYWG
                     DRPPEGCFHMELIFQPAHTPLPPLRMSFSLAKMLGEDPLPFALAVVPRSYWGRRANAI
                     HLDGLMPPRAVHVLQGQIERFTGIEVTDSHLSGIRKHERMPAIVFTQLLVTWPILEHI
                     ANLLEKRAASAGVTPVSRIEPQDSDDELP"
     gene            complement(9488..10126)
                     /locus_tag="PK144_03845"
     CDS             complement(9488..10126)
                     /locus_tag="PK144_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNF64890.1"
                     /translation="MSSVRPTNAAPTGLKPLVFLLCLLPLGLLIWRGFEAGLGANPVE
                     TLEHATGDWALRLLLLTLCATPLRQLSGWSWPLRLRRMLGLYTFFYASVHMALYFTLD
                     LELDFYMLGNEVLKRPYLSVGFLAWALLIPLALTSTDRMIRRLGRRWKSLHRSVYVIG
                     VLAVLHYLWLVKADVREPLLYGAILIVLLGWRAWRERRDRKGGRRSQAVRTV"
     gene            10308..13037
                     /locus_tag="PK144_03850"
     CDS             10308..13037
                     /locus_tag="PK144_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNF64891.1"
                     /translation="MFRPNPCPQRARGALRVLAIVLLLIGLGTPLSLPAQTDPPPPRA
                     LADLLNDWKATLDGLGRQLAAGRSEEADLEKIRTEAERVRSAVQASRGEALGKVAAAQ
                     QLVEALGPPPAEGQPPEAKDVARQRQQLGADLGAAEGRAKQLDVLEARANGLINDASR
                     AIRERIASSLLARGPSTLEADVWSLGGTQFALGLQQLSAGPVARWQSNGFAPLFAKSA
                     GTLFVLAIGLAAAWWLRRWLLARYGRNDIEAAPSYSRRVGAALAEGCCRALLPSLAAA
                     AVALTLHVTGLVEAPGRPMLIGFTVAVIFYVLVTGIDSAALAPSAPAWRLTGLDDRSA
                     RALSRRLNLIAAVFAPLLLASLAAPALNLAEEGRALVVCIGSVMLGLALLTVTRRDTW
                     QRTEGEAPAVEAADALDPLAGQRPEGEAAPNRSTLAEPFWALMRFAVALIAVAAPVAS
                     LLGYVNLARYLLENLVWTALGLAGFVIVRRLIGEAVDRLVLVEGAHRGPLARMFALKA
                     GSSQTLAFWLMLLIDLTLLALAGVLLLLRWGVRWADIQNGLNALLYGFKLGGMTLSLL
                     DGLLAMALFAAVLAASRVSQRVLEERVLPHTHLDAGLRNSLKSGIGYVGLAIAIAVAV
                     STLGIDLSKAALLAGALSVGIGFGLQNIVSNFVSGLILLAERPIKVGDWVVVGTNEGT
                     VRRINVRATELETSQRASVIIPNSMLLSQAVLNWTHKDTFGRVEILVGVAYESDPEAV
                     QRILLDCASVHPDVSRWPAPSVMFKAFGASSLDFELRAFLTNVELKGRVASDLRFAIF
                     KAFREHGIEIPYTQTEVRLRDLERIETLVDKLGPTLRHAQGSRPAAVVTTRPVPPVPA
                     GIEASGIDTGPALPVDNGAQAAAAAAALYAVSTLAEANDPRRDDDDGPDRGSR"
     gene            13066..13323
                     /locus_tag="PK144_03855"
     CDS             13066..13323
                     /locus_tag="PK144_03855"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNF64892.1"
                     /translation="MQLFERGSPWIIERPAGTAVSVCRCGHTGNPPYCDGSHADHPGC
                     SPALLRVADDGQLWLCGCGRSAKLPLCDGSHRRPKPAGDGG"
     gene            13390..13593
                     /locus_tag="PK144_03860"
     CDS             13390..13593
                     /locus_tag="PK144_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64893.1"
                     /translation="MDRRDLPAPVRRHAQNAQKKAGALFALRLKESIATTRYRGETSD
                     RRMLQCSIRMPRQPVNVGIVIIS"
     gene            13864..14196
                     /locus_tag="PK144_03865"
     CDS             13864..14196
                     /locus_tag="PK144_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64894.1"
                     /translation="MPRPLDPLLAGLCSLAMLGPVVAEPGCGLSVDELHRIAGAPARE
                     HYDRVTALPAGLPPLAASDWPAPTFVERHEGRCRVLYGGNKAGPAATVDIDTRGNILG
                     IRSYHAAK"
     gene            14279..16327
                     /locus_tag="PK144_03870"
     CDS             14279..16327
                     /locus_tag="PK144_03870"
                     /inference="COORDINATES: protein motif:HMM:NF017889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007160 - cell-matrix adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nidogen-like domain-containing protein"
                     /protein_id="HNF64895.1"
                     /translation="MKPFSAVFRVHRPQPRAVLSAALLAALAPAVAGAASLSANYDAS
                     YTLGGNDVTAALIDNFDAAMAMWEAALPDSSINLSIDVRLANLGGDGNAGLTTYLTRN
                     ATSGQVNSARIDFNNNTSLWYLDASPTNNDEFDMVQYFRNYNPANTAEIDAGRVGNAN
                     VAAAQGNWDFLSVAVHEIGHALGFGQNGSDPANYTLFGTEVGDGDVDIDAGFNVLFEQ
                     NRNPITAVPVTGSHIDGSGANSDYNTASLADPGFARGQRSLISDVDTLAIGSLYDLPA
                     DGVKLNFDYDQSVLLTGLGGPAGYGELTQGRNDDGSSGLLDLPFEINFFGNVYDALYV
                     NNNGNLTFNSPLGTFTPESFPVANQPMIAPYWGDVDTRCAACGEVYIAAPDENTVVVT
                     WDQVGYYSNHSDLLNTFQAVLRKRADTNTAGQTGNNFDVEFRYGSLNWTTGDASGGTG
                     GFGGTPAQAGFDAGDGINFLALPGSLTGGITSIEISGNTTPLVPGLWTFAIRNGALPG
                     STPDNPLLPVVTDTGFVFDFNVGDINDQIFIDPIIAIGYDYFVDSGPNFASVLLPTGI
                     GDDLFDLWLFDALLNDWVDSGVDIAGGTQHFFAPGGVSRFRILGIEIAALLDPDDPTA
                     FVTGLTFAGTGQVSMRQVAIRFDTDQPIGIPEPAGLALLAFGLLTMFGRCAHGRRFAA
                     "
     gene            complement(16364..>17117)
                     /locus_tag="PK144_03875"
     CDS             complement(16364..>17117)
                     /locus_tag="PK144_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF64896.1"
                     /translation="ATALLVPASLAAVPALLARARSYGDGGPSRFELLPRLALDTACR
                     HLPGCRTPFEPLPQWMLLAGFDGRDPRVQAEVEALLAAALEAGEIVDAVLAQSGAQAD
                     ALWRLREAIVEAQPLLGPSLKHDLAVAVADLPAFVEQACAALAAELPGVRPYVFGHAG
                     DGNLHFNLSPPPDMAPAAFLDAGPRLRELLHGLVAGFGGSFAAEHGIGRIKTGEMARW
                     KSPVELELMRRVKAALDPEGILNPGKVLPPPA"
BASE COUNT         2668 a         5635 c         5828 g         2886 t
ORIGIN      
        1 ccgtctggcc ggtatgcagc cgggtgcgcc gctcgataaa gccgagcacg tcggcgacgc
       61 ggtagcggat cagccggccg ctacggatgt acggcaggtc ataccgcccg taggtgcgcc
      121 agttcgccag ggtttccggt ttcaggccga gcgcggcggc agtgctcgcc gtgtcgagca
      181 gtgcggtcgg cggcagggcg gacaggtctg gcgggatgat ggctgcggcg gcattcgccg
      241 gcagtgcagg ggtgtcgctg gtgctcatta gtgtgccctg tcattggtaa gtgatgacag
      301 ggcagacgat ggcggggcga aacaacgaag tcgtcccaag taatcattac ccggcaccga
      361 gtaatcggca tgccgggtaa tacggattac tcggaagcgt cgtcctcgat tactcaggag
      421 tcctcatcaa ttactcggga gtcggtcaag gctctggcga tatgctccga catggtcttg
      481 attgacaagg gcgtggcacc gtcaggccac cagcgatgcg ccatatcgtt gacggcttgt
      541 gcaagtcgtg cggcgattac acgaccggtg ccgtcgcggt agtcgctcgg gttcgcagcg
      601 agcagataca gggcagctgc gagcgtttcg gcctttttgg tagcgtgccg ggctatttgt
      661 acggcagatc gcacattcgg cgcgtcatgc gctgtctgtt gctccgtctc tgtctgccgt
      721 tcgcgcaaat cgtcgcgctc gcgctccgca gccgccgcgc gggtctctgc tgcggcagca
      781 gcttcaatcg cttttcgacc ttgcgtctgc gcatcatgaa gcattgcttt gatgtcattg
      841 ctttcttgtt gtgtggcttt tgtgttgctg gtcgagcatg aatcagaaag tccattgtcg
      901 gtgttctcat ttatccattt gaataaaaca attatcctat ccaagaatgt ttttgattga
      961 tagctctcaa gaaacgggtc actgcctcgt gaaatattcg caaacaattc tctcgccaaa
     1021 ccttcgctaa taatgtcgga gacatcaacg ccttttgcgt atattttctt tctgtcggat
     1081 aacaaagcat tggcgacaag ccttaattca tttttagaaa gcccattaat tttgcattca
     1141 aattctcttt tgaaatcatc aagtaacatc acgcgccctc acaagcgcct cactagaaag
     1201 ggcgcccgct ccgggccggc gtgaggaacc ggcttttcct gccgtcgcag taggagcggg
     1261 ctttatcgtg ccggctcgcg gccggcttgc tggtggtcag tcgcgtccgg cttccatcgc
     1321 gaggttcagc gcggcaaggt cggcgagccg gcgatcggtg ttggcgtcaa gctgcaacag
     1381 gctcatgggc ggcgtggctc cagtggcggg cgacaggatc aggccgggtc cggccagaac
     1441 tcggcggcga gcacgtcgtc gagcgcgaag aggcaatctt ccgggaaggt cgcggccggc
     1501 agccgggttt cggcgacagc cagccgcagc gcgcgcggcc atgtctttgc gagcacttcg
     1561 ggcaggcggg cgcgcaggct cggactggcg tcgagcagat cggcgatgcc gttgcgctgc
     1621 ttgatgatgg atacctccca gctcgcgccg cgccgctcgg gctggaagcg ccacttcagc
     1681 aggtgcagca gcagttcggt catgcggctg ttcagcgcgt tgcgctccga tgcccccatg
     1741 ctctccagtt cctcggcgat gtgctcgaag tccacttcgg caaggtgccc ggcgcggatc
     1801 agcgcggcgt tgccggtcgc ccaggcatac aggtcggtgt cgtacaggtc cgtgctcatg
     1861 gcgggcgcct cagccggccg tgccggcatt gcgggtgatc ggaaccacgg ctgcggattc
     1921 gcgtacaccg gccgctttca gcatgaagtc ggtgatgcgc tgcatcggtt cgcgcagccg
     1981 ttcggtgtcg atgacgatat acccggccgt cacgtcggca ccgctcttgt ggttcagcag
     2041 ccgtttcagc gcatacgccg ggatgtcgag ggattcggcg atggtgatga aggtccggcg
     2101 caggtcatgc gggcagaagg tgatgccggt ttcggtcgcg atgcgctcga tcagccggcg
     2161 cacgtcgcgc acatgcggga acagcagcgt tgccggatcg gtgccggcgg cctcgatgcg
     2221 gctgcgcagc agcgtttcga gccggtcgga cagcggcagg gtatgcgggc ggcggttctt
     2281 ggtgtccggc accgtgaggg tgcgggcggc gaggtccacg tcggcgacac gaagctgcgc
     2341 ggcctcgttc ttgcgcaggc cggtgagcag cagcagttcg aggtaggcgc gggcatcgtc
     2401 gtcgtagcgc ggaccgcgca ccggctgcac gcgccagagc gccgcatgca ggtcggcaag
     2461 ctgcgtcggc ttgatgacgg tctggcgccg ctcgatgcgg tgccatgcgc gcgtcgctga
     2521 caggcgccgg gtcgggatgt cggacaggat cggtgcgccg tcctcgtcct cgtacttgcc
     2581 ggcggcgtag gtcatcagcg cccgcagcac gcgcatcgca tagttggctt cggcctcgct
     2641 gccgcggcgc acgctgcgcc cgttcacgac gtagctgcgc ggctcgctca ggctggcgtg
     2701 cttgcgttcg accatgtcgc ggctgatcga cagcagcggc ttgtccttcc actcggcgag
     2761 gttcacttcg acttgtcggc ggtagccggc gacggtgcgc ggcttcaggt cgcgcccctt
     2821 caggtagtcg tcgagcacgt cggccagcgt cagcttgcgc gcccggtcgg cgcgcttgac
     2881 ggcgttcggg tcggtgccgt cgttgatctg cacatgcgcg ccggttgctg ccttgcgcgc
     2941 ctgttcgacg gtcatttccg gaaagcggcc cagcgttacg aaagtcggct tcccattcac
     3001 ccgccgttgc aggatgaacg acttcacccc tttatccgta acgcgaattg ccagcgccgg
     3061 gcattcggtg tcgagcaggg tttcgcgctt gccggcagcc gctgcaggca cggcctccag
     3121 agcacgcttc gtgaacttga ttcgggtcgt cggcatcggg cttcaccatg tcgggcatac
     3181 gccgggcata cgccgggcat acggtttggg tggtttcgag tgatgcccgg tgatcgaagc
     3241 atgcgcccaa gtgactgaaa tatcaacgat cagtgatgac gggtgatgat gggtgacggg
     3301 gcggaaatca gactcgaaat cggatgggcg gtaacacgcc acnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnggcgtgta agcatcggag cgtgatcgac gatttttcgc
     3481 ctcgccggaa ccaagccggc gagcatgggt ccatgtgttc gttgcgatga ttcaggccgt
     3541 cattcagatg cgtcgacgct ttgcggatcg gcgggagtgt ctgtctgacc gcgtaattgc
     3601 caacaacaag accaacagga gaacctccca tgtcgctcgg tacgattctg ctgatcgtgt
     3661 tgatcctgct gctgatcggt gccattccag cctggccgca cagtcgtgac tggggctatt
     3721 acccgagtgg cggtctgggg ctggtcgtgg tggttgtgat cattctggta ttgatgggca
     3781 gaatatgacg gacgtgcatt cgccgaattt ggcgtgatgc acgcaaaccg catggtctga
     3841 tccatctcca ttcgttcaac cgcggcctgt ccggtcattg ccggacaggc cgcggttttt
     3901 gtgtatgcca attttttgtc cggaaatcct gcgggtgtgc agacggggct gcagggcctg
     3961 caaatgcctt ccctggcact gcagctcgcc gtgcaccgca aaccggtttc acgcggaatg
     4021 tcaccgggat cggggctcgc cgtctgccgc ccggtctcca ggcccggagg cccggtttgc
     4081 agcgccgccg cacacgactc cctggtattt ctgccgagcg cagttgcgcc cgcccgccag
     4141 gcccccgccg tggacgtgcc gcaccgcacc agactacgat cccccgaccc gcaccggccg
     4201 ttcgcgccga ccttccccgc aagcgctgct cccagcaagg cccgtccatg cccgtgaccg
     4261 atgacaaggg gcttcgtgcc cgcgtgaagc tgttcggcaa tctgctcggc gaggtgctgc
     4321 gcacgcagga gtccgggcag gtgttcgatg ccgtcgaggc cttgcgcaag ggcttcatcg
     4381 agctgcgcac cagtgaggac ccggcgcggc gcgcgcagct gatggcgctg atcgaggcgc
     4441 tcgacccgca gacgctgacg cacgtgctgc gcgcgttcag cagttacttc ctgctcgcca
     4501 acatcgtcga ggaggactgg gcgcatcagg agcgtcgcca ccgcgtcgcc agcggtgagc
     4561 cgctgtggcg cggcagtttc gatgacaccg tgcgcgaact cgtcgccgcc ggcgtcagcg
     4621 ccgagcagat gcagggcctg ctcgacagcc tgctgttcca gccggtgttc accgcacatc
     4681 cgaccgaagc caagcgccgg gcgctgctgg aagcgcagcg gcgcatcttc gtgatggcca
     4741 agaagctcaa cgatgccggg ctcggcggct atgcgcgtga ggcggtcgaa cgccagttgc
     4801 tgaaccagat ccagatcctg tggaaaaccg acgaggtgcg cgtgcacaag ccgcaggtgc
     4861 gcgacgagat cgtcaacggc ctgtactact tccgcgagac gctgttccac gcgctgccgc
     4921 acatttaccg caacctcgaa cgggcgctgg ccacgaattt cggcgaagcc gcggcggcga
     4981 tccgcgtgcc gagtttcatc cgctatggct cgtggatcgg cggtgatcgc gacggaaatc
     5041 cctttgtcac gcacgtggtc accgcgctcg ccgtgcgttt gcaggcgcgt acggtgctgc
     5101 gcgaatacgt gcagcggctc gaagaccttt cacacctgct gacgcattcg agttcgctgg
     5161 tgcgcccgtc actggcattt gccgagcggc tggcggccga tgcgccgctg gtgccggagg
     5221 tctatgccga cgagccgcat cgcttcagcc aggagccgta ccggcgcaag ctgttcctga
     5281 tgcgcgcgcg gctgctgcgc aatctcgacc ggctgaatgc agcgatcgac gggcatgagg
     5341 caccggttgc tgccggttat gccagcgagc gcgattttct cgaagacctg tacgcgatcc
     5401 gcgattcgct gcattcgcac ggcgacggca atctcgccga tgcggagctg aaggatctga
     5461 tccggctcgc cgagagtttc gggttttatc tcgcgcaact cgacgtgcgt caggaatcga
     5521 cgcgccatac cgatgccgtg accgaactgt tcgcgcgcgc gccgaacctg cccgattacg
     5581 cggcgctgtc cgaggacgag cgcctgcagg tgctcggtga actgctcgcg catccgggca
     5641 cgccgctgct gtatgccgag gatctctccg agaacacgcg cgagattctc gcggtattgc
     5701 ggaccgttgc cagcctgcgc gacgagatca gcccgaatgc cttcggcgcc tacgtgatct
     5761 cgatgacgca cgcggcgagt cacgtcatgg aggtgctgtt cctcgcgagc ttcgccggac
     5821 tcgccggccg gcgcggcgat ggcagctggt tctgcgatct gcgcgtttcg ccgctgttcg
     5881 aaaccatcgc cgatctcgcg cacatcgagc cggtgctcga acgtctgctc gacaatccga
     5941 cctaccgcga actgctggcg gtgtcgggca acgtgcagga ggtcatgctc gggtattcgg
     6001 attcgtgcaa ggacggcggc atcctggcct cggcctggaa cctgtaccgc gcgcagaaga
     6061 agatcgtcac gatcacggcg cgccgcgaca tccgctgcaa gctgttccac ggccgcggcg
     6121 gcacggtcgg tcgcggcggc ggcccgacgc acgatgcgat cctcgcgcag ccggccggca
     6181 ccgtgcaggg acagatcaag ttcaccgagc agggcgaggt gctgtcgtcg aagtattcga
     6241 atccggaaac cgcggtctac gagctgacga tggcgatcac cggcctgatc aaggccagtc
     6301 gctgcgcggt gaccgaatgc attccggacg aaccgcagtt cctgacggtc atggaacagc
     6361 tcgcaaagga cggcgagcag gcctaccggc agctgaccga tcacacgccg cgtttcatcg
     6421 attacttcta cgaggcgaca ccggtcaccg agatcgggct gctgaacatc ggttcgcggc
     6481 cctcgcaccg caagaaggcc gatcgttcca agtattcggt gcgtgcgatt ccgtgggtgt
     6541 tcggctgggg gctgtcgcgg cagatcattc cgggctggta cgggctcggt gcggcgctgt
     6601 cggcatatcg cagcggcgac agcggccggc tcgatacgct gaggcgcatg tatgccgaat
     6661 ggccgttctt ccgctcgctg ctcgcgaatg cgcagatgtc gctcggcaag tcggaaatgg
     6721 gcatcgcgca cgagtacgcg cagctctgtc tggagtccgg caccgcggat gacatttacg
     6781 gcacgatccg cagcgagcat gacgaaaccg tcgcgcaggt gctggcggtt gccggcatcg
     6841 aggaaatcct cgacgagaat ccgcgtctga agctgtcgct ggcgcggcgc gatccgtatc
     6901 tggacccgat caaccacatc cagatctgcc tgctgcgccg ctaccgtcag gccgaaggcg
     6961 gcggcgaggc cgagcgggcg ctgtggctca atccgctgct gcgctcgatc aacgcgctgg
     7021 cggcgggcat gcgcaacacc ggctgaggcc cggcagcggg cggcgtccgt gtcgtcgccc
     7081 gcggacctct gcgctcacgc cagccgcagc agcggatcct gccggtagaa ggcccgcagc
     7141 tgcgcgtaga ccggcggata cgtggcgttc agcaccgccg gcgtctcgaa gaaggcttcg
     7201 ctcagcaccg cgaagaactc ggcggggctg tcgctggcgt agggatcgat cgcggtgtcc
     7261 tcgccggcat cgacgtgcgc gcagaagtcg tcgtaggcgc gcgtgaagat gtcggcccac
     7321 gcgcgtaccg acatgccgcg atgcagcggc ggcaggccgt tggcctcgcc gttgagcagg
     7381 tcgagcttgt gcgcgagttc gtggatgacg acgttgaagc cgtcggtggc ggtttcgctg
     7441 tcgagcacgt cggcccagga caggatcacc ggcccgcgtt cccaggcctc gccgatcagc
     7501 ggccggcgca gctgatgcac gacgccggcc tcgtcggtga agtcctgccg ggccatgaat
     7561 tcgccgggat agacgacgat cgccgaccag ccctcgtacc agtcgaggcc gaggttcagc
     7621 accggcaggc aggcgaccgc ggcgatgcgc cagcgcatcg gcggcgtcag cgtcaggccg
     7681 gcagcggcct cgaaggtttt ctcgtgcagg aacagcgtcg cgagccggcg caggcgctgc
     7741 tgatccgcct gcggcaggcc ggcgaggact gcctgcgcgt cgcaggccgc ctgccaggtc
     7801 ggcgcatcgg cttcgctctg gcggagcacg cgccggcgac gccagtcccg cagccagccc
     7861 atcgtgcgtc agtcgcgtcg ctgctgcgtc tgccggtcgc gctgctggcg cgccagccag
     7921 cgctcggacc agcgcgccag cgcccagatc ggcgagccga tcacgaacac cgcgagcagc
     7981 aggcacagga tcacggcgag ccaggccggc agccgctgcc agccctcgtt gaccagccag
     8041 accagcgccg ccgtcatcag ggccgtcagc agcagggccc cgaccagtgc ctgccgctca
     8101 cgggagttca tcgtcgctgt cctggggctc gatgcgcgag accggcgtca cgccggcgga
     8161 ggccgcgcgc ttttcgagca ggttggcgat gtgttcgagg atcggccagg tcaccagcag
     8221 ctgcgtgaac acgatcgccg gcatgcgctc gtgcttgcgg atgccggaga ggtgcgagtc
     8281 ggtgacctcg atgccggtga agcgctcgat ctggccctgc agcacgtgca cggcgcgcgg
     8341 cggcatcagg ccgtcgaggt ggatggcgtt ggcgcgccgc ccccagtacg agcgcggcac
     8401 gacggccagc gcgaacggca gcgggtcctc gccgagcatc ttggcgaggc tgaaggacat
     8461 gcgcagcggc ggcagcggcg tgtgcgccgg ctggaagatc agctccatgt ggaagcagcc
     8521 ttccggcggc cggtcgcccc agtattcctc gttggcgagc agcttcagct tgctgcggtt
     8581 gatcttgatg acgacgttgg cgtcggtctt ctggaactcg atccagcggc ggaacgccgc
     8641 ttcgcgcttg cggatctcgt cggccagcgg ctcgacgcga tgcccgcgga actgcgtgtc
     8701 gcgcgcgatc ttccagccga gcttgatgtc gcgggccgga tcgacgaaca ccgtgtaatc
     8761 gagcagtgac gtgaactcgg gatacagcgc gtgcaggcct tcgagcacga ggatcggctt
     8821 cggcgagaac ggctgcggcg caatgaaggt gccggtgccg tgatcgtagc ggcgcaggtg
     8881 cacggattcg ccgcggcgca gcgcttcgag gtgctcgcgc agcaggccga ggtggttcat
     8941 ctcgggatcg agcggcgaac ggccggtgcg cgcgcgcgtg gcgcggtcct ccttgtggta
     9001 gccgtccatc tcgatcgtgc cgacgatgtc atcgccgagc atgcgccgca gaccgttggt
     9061 gtaggtggtc ttgccggcac tgctgtcgcc ggcgacgccg atgatcagcg gcggaccggg
     9121 tacttcaagc agggagcgca gcgtctgggc gcggcgccgc gaaagggacg cggcgagcgg
     9181 aatgggcatc agcgactccg tagcgtcggt atcgccggac agtgtggaca gcggggcggg
     9241 gggggatcaa ggccgcgacc ctggaagtcg ggcaaaacaa cgggggaaga ccttgcggcc
     9301 ttccccacca aacaagacgg tacttccgtt gtacttggca aacctgcccg cagcgggaac
     9361 ggacagtcgc ggttttaata gaagcaaatg atgggccagt ttataaaaaa ctttattttc
     9421 aattacttat tatgtttcgc ccgggcttgc cgtaaggaat cctgacaatg tgatgccgct
     9481 cgccggttca gacggtgcgc accgcctgcg agcgccggcc gcccttgcgg tcccgccgtt
     9541 cccgccacgc gcgccagccg agcagcacga tcaggatcgc gccgtacagc agcggctcgc
     9601 gcacgtcggc cttgaccagc cacaggtagt gcagcacggc gagcacgccg atcacgtaga
     9661 cgctgcggtg caggcttttc cagcgccggc cgaggcggcg gatcatgcgg tcggtcgagg
     9721 tcagcgccag cggtatcagc agcgcccagg ccaggaagcc gacgctcagg tagggacgct
     9781 tcaggacctc gttgccgagc atgtagaagt cgagttcgag atcgagcgtg aagtacagcg
     9841 ccatgtgcac gcttgcatag aagaacgtgt acaggccgag catgcgccgc agccgcagcg
     9901 gccagctcca gccgctgagc tggcgcagcg gtgtcgcgca cagcgtcagc agcagcaggc
     9961 gcagcgccca gtcgccggtg gcgtgttcca gggtctcgac cggattggcg ccgagcccgg
    10021 cctcgaagcc gcgccagatc agcaggccga gcggcagcag gcagagcagg aacaccagcg
    10081 gcttgaggcc ggtcggggcg gcgttcgttg ggcggacaga gctcatcgtg gcggacaagg
    10141 ggcggattcc tgcagcaagg gcgttaccgg atggtacggg cggcaaggcc gtgcggatgc
    10201 agcggcccgg gctgtggcgg gggcgccgtc gtcatccgcg tgcggcaagt gcttgtgcta
    10261 gcgtatgtgc ctttgtctga aagcccgtca ttgccgagcg ttccttcatg ttccgtccga
    10321 atccctgccc gcagcgcgcc cggggtgcgc tgcgagtcct cgccatcgtc ctgctgctga
    10381 tcggcctggg cactccgctg tcgctgcccg cgcagaccga tccgccgccg ccgcgcgcgc
    10441 tcgcggacct gctcaacgac tggaaggcca cgctcgatgg cctcggccgg cagctcgccg
    10501 ccggcaggag cgaggaagcc gatctcgaga agatccgcac cgaggccgag cgcgtgcgca
    10561 gcgccgtgca ggccagccgc ggcgaggcgc tcggcaaggt ggccgccgcg cagcagctgg
    10621 tcgaggccct cgggccgccg ccggccgagg gccagccgcc cgaggcgaag gacgtggccc
    10681 gccagcgcca gcagctcggc gccgatctcg gcgccgccga aggccgggcc aagcagctcg
    10741 acgtgctcga agcgcgcgcc aacggcctga tcaacgacgc ctcgcgcgcg atccgcgaac
    10801 gcatcgcatc gagcctgctg gcgcgtggcc cgagcacgct cgaagccgat gtctggtcgc
    10861 tcggcggcac gcagttcgcg ctcggcctgc agcagctttc ggccggtccg gtggcgcgct
    10921 ggcagtccaa cggcttcgca ccgctgttcg cgaagagcgc cggcacgctg ttcgtgctcg
    10981 cgatcgggct ggcggcggcc tggtggctgc ggcgctggct gctggcgcgc tacgggcgca
    11041 acgacatcga ggcggccccg agctacagcc gccgcgtcgg ggcggcgctg gccgagggct
    11101 gctgccgggc gctgctgccg agtctggcgg cggccgcggt cgcgctgacg ctgcacgtga
    11161 ccgggctggt cgaggcaccg ggccggccga tgctgatcgg cttcacggtc gcggtgatct
    11221 tctacgtgct ggtcaccggc atcgacagcg ccgcgctggc gccttcggcc ccggcctggc
    11281 ggctgaccgg cctcgacgat cgcagcgccc gggccttgtc gaggcggctg aacctgatcg
    11341 ccgcggtgtt cgcgccgctg ctgctcgcgt cgctggcggc cccggcgctg aacctcgccg
    11401 aggaggggcg ggcgctggtg gtgtgcatcg gcagcgtcat gctcgggctc gcgctgctga
    11461 cggtcacgcg ccgcgatacc tggcagcgca ccgagggcga ggcgccggcg gtcgaggccg
    11521 ccgatgcgct cgatccgctg gccggccagc gcccggaggg cgaggcggca ccgaatcgct
    11581 cgacgctggc cgagccgttc tgggcgctga tgcgctttgc ggtcgcgctg atcgcggtcg
    11641 ccgcgccggt tgcctcgctg ctcggctacg tcaacctcgc gcgctacctg ctcgaaaacc
    11701 tggtctggac cgcgctcggg ctggccggct tcgtgatcgt gcgccggctg atcggcgaag
    11761 ccgtcgaccg gctggtgctg gtcgaggggg cgcaccgcgg gccgctggcg cgaatgttcg
    11821 cgctgaaggc cggcagctcg cagacgctgg ccttctggct gatgctgctg atcgacctga
    11881 cgctgctcgc gctcgccggc gtgctgctgc tgctgcgctg gggcgtgcgc tgggccgaca
    11941 tccagaacgg cctgaatgcg ctgctgtacg gcttcaagct cggcggcatg acgctgtcgc
    12001 tgctcgatgg cctgctcgcc atggcgctgt tcgcggcggt gctcgccgcg agccgcgtca
    12061 gccagcgcgt gctggaggag cgcgtgctgc cgcacaccca cctcgacgcc ggcctgcgca
    12121 actcgctcaa gagcggcatc ggctacgtcg ggctcgcgat cgcgatcgcg gtggcggtct
    12181 cgacgctcgg catcgacctg tcgaaggccg cgctgctcgc cggcgcgctg tcggtcggca
    12241 tcggtttcgg cctgcagaac atcgtcagca acttcgtctc ggggctgatc ctgctcgccg
    12301 agcggccgat caaggtcggc gactgggtcg tggtcggcac caacgagggc acggtgcggc
    12361 gcatcaacgt gcgggcgacc gagctcgaaa cctcgcagcg cgcctcggtg atcatcccga
    12421 actcgatgct gctgtcacag gcggtgctga actggacgca caaggacacc ttcggccgcg
    12481 tcgagattct ggtcggcgtc gcctacgaat ccgaccccga ggccgtgcag cgcatcctgc
    12541 tcgactgtgc gagcgtgcat ccggacgtga gccgctggcc ggcaccgagc gtgatgttca
    12601 aggccttcgg ggcgagttcg ctggacttcg agctgcgcgc cttcctgacc aacgtcgagc
    12661 tgaaggggcg cgtcgcctcg gatctgcgct ttgcgatctt caaggccttc cgcgagcacg
    12721 gcatcgagat tccgtacacg cagaccgagg tgcggctgcg cgatctcgaa cgcatcgaga
    12781 ccctggtcga caagctcggc ccgacgctgc gtcacgcgca gggcagccga ccggcggcgg
    12841 tcgtcacgac ccggccggtg ccgccggtgc cggccggcat cgaggcttcg ggcatcgata
    12901 ccggtccggc gctgccggtc gacaacggcg cgcaggcagc ggcggcggcg gcggcgctgt
    12961 acgcggtgtc gacgctcgcc gaggccaatg atccgcgccg ggacgacgac gacggcccgg
    13021 accgcggcag ccgttgagcc ggcgtcgagg ccgggggagg tgaggatgca gctcttcgaa
    13081 cgcggcagtc cctggatcat cgagcgcccg gcgggcacgg cggtcagcgt ctgccgctgc
    13141 ggccacaccg gcaacccgcc gtactgcgac ggctcgcacg ccgatcatcc cggctgcagc
    13201 cccgcgctgc tgcgcgtcgc cgatgacggc cagctgtggc tctgcggctg cgggcgcagc
    13261 gcgaagctgc cgctgtgcga cggctcgcac cggcggccga agccggccgg ggacggcggc
    13321 tgaaccccgc tgccgtcgcg atccgcagct gagccggacc acggcgtccg gacggaatcc
    13381 ttcgtctgca tggaccggcg cgaccttccg gcgccggtcc gtcgtcatgc gcaaaacgcg
    13441 caaaaaaaag ccggtgcgct gttcgcgctc cggctcaagg agagtattgc gaccaccaga
    13501 tacagaggag aaacatccga caggaggatg ttgcagtgca gtatccggat gccccggcaa
    13561 ccggtcaacg tgggaatcgt gatcatttct tgattgtcga ctgaattttt cttaatcctc
    13621 caggccctgc cgaccgtcgg tcgactttac agataatcgt gtggccggct ttcgtgcgtt
    13681 gagctgtgtc tgcaaaaaaa cgatataaaa catgatgttg atttgatttt ccgcattgcg
    13741 gaaaaacatt ggaacgatcc ttgtactacc tcacctgtca gggccgacac cgctgaatcc
    13801 cttcagggat gctgatgtgg tagcggccgg atcccgtgcg gagcctgccg cgtggagcgt
    13861 ccgatgcctc gtccgcttga tcccctcctt gccggactct gcagcctcgc catgctgggt
    13921 ccggtggtcg ccgagcccgg ttgcgggctg tccgtggacg aactgcaccg catcgccggt
    13981 gcacccgccc gggagcatta cgaccgcgtc accgccctcc cggccggtct gccgccgctc
    14041 gcggcctcgg actggccggc gccgaccttc gtcgagcgcc atgagggccg ttgccgtgtg
    14101 ctgtacggcg gcaacaaggc cgggccggcg gcgacggtcg acatcgacac ccgcggcaac
    14161 atcctcggca tccgcagcta ccacgccgcc aaatgacgat aaagcccgcc ccggcgcgcc
    14221 cggtggtgcg ccggaggcga gcgcgaacac ccgccaccgg cgggaggagc gcactccgat
    14281 gaagccgttt tctgccgttt tccgcgtgca tcgaccgcag ccccgggccg tcctgagcgc
    14341 agccctgctc gctgcgctgg cccctgccgt ggccggggca gcgagcctga gcgccaacta
    14401 cgacgccagc tacacgctcg gtggcaacga cgtgaccgcc gcgctgatcg acaacttcga
    14461 cgcggcgatg gcgatgtggg aggccgcact gccggacagc tcgatcaacc tcagcatcga
    14521 cgtgcgcctc gccaacctcg gcggcgacgg caacgccggc ctgaccacct atctgacccg
    14581 caacgccacc agcggacagg tcaactcggc gcgcatcgat ttcaacaaca acaccagcct
    14641 ctggtatctg gacgcctcgc cgaccaacaa cgacgagttc gacatggtcc agtacttccg
    14701 caactacaac ccggccaaca ccgccgagat cgatgccggg cgcgtcggca acgccaatgt
    14761 cgcggccgcg cagggcaact gggacttcct gtcggtggcc gtgcacgaga tcgggcacgc
    14821 gctcggcttc ggccagaacg gcagcgatcc ggccaactac acgctgttcg gcaccgaggt
    14881 cggcgacggc gatgtcgaca tcgatgccgg cttcaacgtg ctgttcgagc agaaccgcaa
    14941 tccgatcacc gccgtgccgg tcaccggcag ccacatcgac ggcagcggcg cgaacagcga
    15001 ctacaacacg gcatcgctgg ccgatccggg cttcgcgcgc ggccagcgct cgctgatctc
    15061 cgatgtcgac acgctggcga tcggctcgct gtacgacctg ccggccgacg gcgtgaagct
    15121 gaacttcgac tatgaccagt cggtgctgct gaccggcctc ggcggtccgg ccggctacgg
    15181 cgaactgacg cagggccgca acgacgacgg ttcctcgggc ctgctcgacc tgccgttcga
    15241 gatcaacttc ttcggcaacg tctacgacgc gctgtacgtc aacaacaacg gcaacctgac
    15301 cttcaactcg ccgctcggca ccttcacgcc ggagtccttc ccggtcgcca accagccgat
    15361 gatcgcaccg tactggggcg acgtggacac gcgctgcgcg gcctgcggcg aggtctacat
    15421 cgccgcgccg gacgagaaca ccgtggtcgt gacctgggat caggtcggct actacagcaa
    15481 tcactccgat ctgctgaaca ccttccaggc cgtgctgcgc aagcgcgccg acaccaacac
    15541 ggccggtcag accggcaaca acttcgatgt cgaattccgc tacggctcgc tgaactggac
    15601 caccggtgac gccagcggcg gcaccggcgg cttcggcggc acgccggcgc aggccggctt
    15661 cgatgccggc gacggcatca acttcctcgc gctgccgggc tcgctgaccg gcgggatcac
    15721 cagcatcgag atctccggca acaccacgcc gctggtgccc gggctgtgga ccttcgcgat
    15781 ccgcaacggc gccctgccgg gctcgacccc cgacaacccg ctgctgccgg tcgtcaccga
    15841 taccggcttc gtgttcgact tcaacgtcgg cgacatcaac gaccagatct tcatcgaccc
    15901 gatcatcgcg atcggctacg actacttcgt cgattccggg ccgaacttcg cgtccgtgct
    15961 gctgccgacc ggcatcggtg acgatctctt cgacctgtgg ctgttcgatg cgctgctcaa
    16021 cgactgggtc gacagcggcg tcgacatcgc cggcggcacg cagcacttct tcgcgcccgg
    16081 cggcgtcagc cgcttccgca tcctcggcat cgagatcgcc gcgttgctcg atccggatga
    16141 tccgacggcc ttcgtgaccg gcctgacctt cgccggaacc ggccaggtgt cgatgcggca
    16201 ggtggcgatc cgtttcgata ccgaccagcc aatcggcatc ccggaaccgg ccggcctcgc
    16261 gctgctggcg ttcgggctgc tcacgatgtt cggccgttgc gcacacggac ggcgcttcgc
    16321 ggcctgagtg ccgcgcgggt cgggcaaacc gctgccgccg tgctcacgca ggcggcggca
    16381 gcaccttgcc ggggttcagg atgccctcgg gatcgagtgc ggccttcacg cgccgcatca
    16441 gttccagttc gaccggcgac ttccagcgcg ccatctcgcc ggtcttgatg cggccgatgc
    16501 cgtgctcggc cgcgaagctg ccgccgaagc cggcgaccag cccgtgcagc agttcccgca
    16561 gccgcggccc tgcgtcgaga aaggccgccg gcgccatgtc cggtggcgga ctcaggttga
    16621 agtgcaggtt gccgtcgccg gcgtggccga acacgtaggg gcgcacaccg ggcagctcgg
    16681 ccgcgagcgc cgcgcaggcc tgctcgacga aggccggcag atcggcgacg gcaaccgcga
    16741 gatcgtgctt gaggctcggc cccagcagcg gctgcgcctc gacgatcgcc tcgcgcagcc
    16801 gccagagtgc atcggcctgc gccccggact gcgcgagcac ggcatcgacg atttcgccgg
    16861 cctcaagcgc agcggcgagc agcgcctcga cttcggcctg cacgcgcggg tcacggccat
    16921 cgaagccggc gagcagcatc cactgcggca gcggctcgaa cggcgtgcga cagcccggca
    16981 ggtggcggca ggcggtgtcg agcgcgaggc gcggcagcag ctcgaagcgg ctcgggccgc
    17041 cgtcgccgta actgcgggcg cgcgcgagca gcgccggcac cgcggcgagc gaggccggca
    17101 ccagcagcgc cgtcgcc
//
LOCUS       DAOQIE010000036        16575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1635_length_16575_cov_7.612954, whole genome shotgun sequence.
ACCESSION   DAOQIE010000036 DAOQIE010000000
VERSION     DAOQIE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16575
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_16575_cov_7.612954"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..394)
                     /locus_tag="PK144_03880"
     CDS             complement(146..394)
                     /locus_tag="PK144_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64897.1"
                     /translation="MRHLPAFSVAVLLAALAAPAQAYHCPVDMKQIDAALAASPKLGT
                     EQLAEVRALRAKGEEQHKAGDHAGAVDSLGKAKKILGI"
     gene            547..1587
                     /gene="queA"
                     /locus_tag="PK144_03885"
     CDS             547..1587
                     /gene="queA"
                     /locus_tag="PK144_03885"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNF64898.1"
                     /translation="MLRSDFHFDLPPELIAQQPPAERGDSRLLALDGTDGRLQDARFD
                     DLPGFLRAGDLLVFNDTRVIPARLHGIKAGSGGRVEVLVERILDARRVLAHLRASKSP
                     KPGTRLWLEQAVGAVVLGRAGELYELGFEDARPVPAILDAHGHIPLPPYIDRPDAPAD
                     AERYQTVYARAPGAVAAPTAGLHFTPAMLERLAAAGIGRAFVTLHVGAGTFQPVRTEH
                     ISEHRMHAESVEVSAGTVAAVAAARARGARVVAVGTTAVRSLESAAAGGELRPFAGDT
                     DIFITPGYRFRVVDALVTNFHLPESTLLMLVSALAGHAQVMAAYRHAVAARYRFFSYG
                     DAMFVRGSRPAG"
     gene            1701..2576
                     /gene="htpX"
                     /locus_tag="PK144_03890"
     CDS             1701..2576
                     /gene="htpX"
                     /locus_tag="PK144_03890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNF64899.1"
                     /translation="MKRILLFVLTNIAVLVVLSITASVLGVDRFLTRQGLNLPALLGF
                     AAVFGFGGSLISLAMSKWIAKSSVGAYVIEVPQNPTERWLVETVQRQAAAAGIGMPEV
                     AIYDSPEINAFATGASRNNALVAVSTGLLSNMSPDEVEGVLAHEVSHVANGDMVTLAL
                     VQGVVNTFVIFLSRVVGFVVDRALSGNRDDEGGVGIGYFVTSLICEILFGFLASMIVM
                     WFSRRREFRADAGGAHLAGRNRMIAALERLRQMHEPSHLPSQMAAFGINGGTVQRLFM
                     THPPLEERIAALRAA"
     gene            complement(2668..4758)
                     /locus_tag="PK144_03895"
     CDS             complement(2668..4758)
                     /locus_tag="PK144_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64900.1"
                     /translation="MSLDSTIRLLLVDRPQADAQPVLRPLRAAGFLVRHWQADRIDTL
                     QHLIDEQTFDLAIVRIGEGVLPIENVRDSIDSAFKDIPVIAVADETAGIDAVQLLNAG
                     ADRIAALNHSNSLPLVARLELGRLNERRQARRWEGLYREAEARAQALLETSRDAIAYI
                     HAGAHIYTNPSYRALFGFDDALVDTTLIDLIHRDSRDAVKSYLRARERNPNAPLGDGL
                     ECRGVRADGSEIRIVLLASAARMNEEFCLQLLVREPRGEQPEVQDKLRYFVEHDTLTG
                     LFNRQYFTELLNRIHTSATTSGSLGGAVLYVLMTDYRGVAERLGLEAVDDLLRGVAEA
                     IQQQVGPQEIVARFSDATFTVHTPLSARGAVAELGERVRQGIENHTIQAGGMLITAKA
                     SIGICMIGDTNSSATQIISYADKACEQARQLGGSQVQIYTPVAPERDSIARRDTILRA
                     FRQAVVEQRVHLYYQPIASFTGARGEFFDACLSASDEQGQPLDLREALPLAETRGLMR
                     QLDRWTVATALAAIAARGAAGAGLPTVFVRVSSNSITDAEYRDWLLQQLAERNLPGSV
                     LVLEVAETVVEPYFADLIQLKQAVEPRGCRIALDGFGGRPYSEQLLRQSQPGFVRIDI
                     ELMEQAQRDAAFKQQLDHLTALAAEQHTQVIMTGVDNPAQMAITWQFGAMLVQGDIVQ
                     PPAPELVFDFSQFG"
     gene            complement(4813..5817)
                     /gene="epmB"
                     /locus_tag="PK144_03900"
     CDS             complement(4813..5817)
                     /gene="epmB"
                     /locus_tag="PK144_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HNF64901.1"
                     /translation="MIAAVAHADDTPATDGSGARLLTDPATLLERLGLDAALLPAARR
                     AAERFPMRVPAGFVARMRPGDPADPLLRQVLPLADELATAGGFGADPVGDAAARVAPG
                     LLHKYAGRALLLVTGACAIHCRYCFRREYPYADTGASPAGWEAAFAHLHADRSIRELI
                     LSGGDPLTLSNRRLRELVQAADAVPQLERLRIHTRQPVVDPGRIDAGLLAALRSRLPV
                     VLVLHVNHAREIDAALVQACARMRAAGVTLLNQSVLLAGVNDAATTLAALSEALFAAG
                     VLPYYLHLLDRVGGATHFEVSEQQALSIMGALRAMLPGYLVPRLVREEAGRPAKTPLC
                     "
     gene            5868..6437
                     /gene="efp"
                     /locus_tag="PK144_03905"
     CDS             5868..6437
                     /gene="efp"
                     /locus_tag="PK144_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNF64902.1"
                     /translation="MATYSTNEFKSGLKIMLDGDPYAIVENEFVKPGKGQAFNRVRVR
                     NLKSGRVIERTFKSGDSVEGADVLDIDMQYLYCDGEFWHFMNPTSFEQLAADATAVGD
                     NARWLKENDLCQLTLWNGVPLSVLPPNFIIARITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTDEVIKVDTRTGEYVSRVKE"
     assembly_gap    6452..6551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6555..7541
                     /gene="epmA"
                     /locus_tag="PK144_03910"
     CDS             6555..7541
                     /gene="epmA"
                     /locus_tag="PK144_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNF64903.1"
                     /translation="MSGPADWRPSCAPEALRLRAGLLARMRAFFEARGVLEVDTPALS
                     RAAVTDPALASFAVDWHGPEPGRLWLQTSPEYAMKRLLAAGSGPIWQLAKVFRDGEAG
                     RRHNPEFSLLEWYRPGFDHHALMDEVIDLVSELLAGRRALAEPERIRYQDAFLQHAGV
                     DPFAADVPALRVACTRHALVPPPGMPEAQVEPWLDLLLSHVIEPRLGCGRLSFLYDYP
                     PAQAALACIRRDETPAVGSRFELYLDGVELANGFHELGDAGEQRARFEADNRARVRAA
                     LPVMPIDEALLAALASGLPACAGVALGFDRLLMLAANAGGLDAVLAFPVDRA"
     gene            complement(7659..8048)
                     /locus_tag="PK144_03915"
     CDS             complement(7659..8048)
                     /locus_tag="PK144_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNF64904.1"
                     /translation="MYDLDPFNEALARSATWIAPVVQANKLFVASLEKLAAFQFGLLR
                     SYAGFGLGRLQAASDVTDVESLKAFSAAQLEAAGALRQKALDDAQAYADLLAGFKAEF
                     EQLIAANSTDFAPKPIVKVARRAASAA"
     gene            8323..9297
                     /locus_tag="PK144_03920"
     CDS             8323..9297
                     /locus_tag="PK144_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNF64905.1"
                     /translation="MDKRVETLLSLEMPYRERMRLQRSVFRGGDGPRVAVVAGIHGDE
                     LEGLYLCHRLAAWLDALAERRPGALLGTIELYPALNPLGLDTLARTVPPYGVDLNRSF
                     PGHARGVLPQRIAAATMTALAGCALVVDVHASNIYLREIPQVRINHVFAESLLPYARR
                     MNLDVIWLHGAVTVLEATIAHSLNEAGVPALVVEMGVGMRLTPVYTEQLITGLLNVWQ
                     GLGVLAADLELAPVTHEPRLAHEDNVHYLNAATSGLFVPEVEHWKAVRAGGLLGHIVS
                     PMEGRRLAEVRSPCDGVLFTLREYPLVYEGSLMARVVADAEPENGESA"
     gene            9294..10286
                     /locus_tag="PK144_03925"
     CDS             9294..10286
                     /locus_tag="PK144_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNF64906.1"
                     /translation="MNTPTLLRMTAPLRDDFDIPYHDVGPVERTPRLALVAGLHGNEL
                     NGVFVLARLAAFLRDVEQGKRPDLRLRERVLLVPAVNVLGVNLLSRVWPFDKIDLNRV
                     FPGLEGGETTQRIAAALLRLTRGAYYRIDIHSSNLDIEELPQVRLYAPNDDERATACL
                     FGLPAVIERPLNKMFMATLVHAWRAHGGENFVLQAGRAGDLQSRHCETLFRSLVAFLD
                     RTGIVEGLTLADDEEDLHYFGLRQSYAVLSEQAGFFVGRIDPGRWVQAGELLGHLYDG
                     FTGDLLKDVHTPVAGLVCSVRRQPLLFEGDLLCRIQTLDDSGNDVEVMLHGQGQ"
     gene            10340..10642
                     /locus_tag="PK144_03930"
     CDS             10340..10642
                     /locus_tag="PK144_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HNF64907.1"
                     /translation="MDTATRTELEAAAFRRLVEHLRERSDVQNIDLMNLAGFCRNCLS
                     KWYLAAAQARGLALDYEGAREIVYGMPYEQWKTLHQREASPEQQTAFAEAIRKVHD"
     gene            complement(10681..11115)
                     /locus_tag="PK144_03935"
     CDS             complement(10681..11115)
                     /locus_tag="PK144_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64908.1"
                     /translation="MPVAAYHRWTYNRQDGRSSATLDEPSDTTGSTPVRNVVEFFNPR
                     PIIGVFVDGLAGCDWQCPGTEDAESAELQDVNLLPSGHLVLTLLLRGADEPTEWIGLN
                     GVLDATDGSMALELGGTRYLAFPPRERERVSALFLKLANRQY"
     gene            11145..12194
                     /locus_tag="PK144_03940"
     CDS             11145..12194
                     /locus_tag="PK144_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64909.1"
                     /translation="MSMSKPVRPGGGRRVGLMLVIVVLATALWYGWQVPELRHRLIYG
                     FEHWVAGLQTRFNIRPPGAEPEPTAGEVELLKPPAALRPGTAPAAAAQAPAAQGVRDP
                     SITDDEALLSGAQPMPTLAAPRSLKFAVGEEAVFGLELLPGIAARGQRVIIGGLPMDA
                     SLSAGARDEMGRWVLDAVALPGLKLRSGAPGHYTLLIRTQPLQRLRVPRPVAEAAVEV
                     TVLPAAGAEARTAAPPAAKPPQPGPGELLDRGRRALELGDLGAARLYLEAAAGQNSAD
                     AALLLAASYDPGRSGDNADVGLAADWYLRARKLGAAEAQPAIDALRSWLQARTEPGEA
                     ERKALAKLSEPAPGR"
     gene            complement(12241..13050)
                     /locus_tag="PK144_03945"
     CDS             complement(12241..13050)
                     /locus_tag="PK144_03945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF64910.1"
                     /translation="MPYPPLPTPRAVVLLLLLSGCAAAPEGPQNTVQVPPERLASAGA
                     RASLQIQRRGELLRELDTTRYVRCVADALIQAIPAAPADWEVVVMRDETPDAWALAGR
                     RLGVTKALLRVTGNQDRLAAVLAHGIAHELLGHTGAHITPSVAASLEGDAALSNEAIA
                     AIGGASQVGAGFAYSASEEAAADGRALTLMGAAGFDPAAASLAWAQLQAYRVREGTSG
                     ERPPPMLLVHPIDRSRIQAMHTPAAAVTTAFDAARLSGRRPDCGTLEESER"
     gene            complement(13155..13448)
                     /locus_tag="PK144_03950"
     CDS             complement(13155..13448)
                     /locus_tag="PK144_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64911.1"
                     /translation="MSDMDPIVGNWYRDLESHACFEVVAFDEDARTVEVQFQEGEVEE
                     IELDAWYELVLEGIEAPDDWAGAFDDVDGEDLGYSDLPTGDGRDRDDPFRALD"
     gene            complement(13588..15525)
                     /gene="acs"
                     /locus_tag="PK144_03955"
     CDS             complement(13588..15525)
                     /gene="acs"
                     /locus_tag="PK144_03955"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNF64912.1"
                     /translation="MSEEKVYPVPAEVAAKAWINDAQYQEMYARSISDPDGFWGEKAG
                     ELLTWFKPWSKVSDCDFKAGRIKWLEGGKLNVSYNCLDRHLETRGDQVAIIWEGDSPN
                     EDRKITYKQLHEEVSRFANALKGLGVQKGDRVCIYLPMIPEAAVAMLACTRIGAVHSI
                     VFGGFSPDALKDRIVDASCAVVITSDESMRGGRAVPLKANADKAAAAAECVKNVVVVK
                     RTGGKIAWTEGRDVWYHELVEAAPADCPAEEMDAEDPLFILYTSGSTGKPKGVQHTTG
                     GYLLYASITHKYIFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPTYP
                     TSTRFWDVVDKHQVNIFYTAPTAIRSLMRDGEEQVKATSRASLRLLGSVGEPINPEAW
                     EWYYRVVGDNRCPIVDTWWQTETGGILITPLPGATALKPGSATRPFFGVQPALVDAEG
                     KELEGATEGNLVIKASWPGQMRTIFGDHNRYVESYLSVYPGTYFTGDGARRDEDGYYW
                     ITGRVDDVINVSGHRMGTAEVESALVLHAAVAEAAVVGYPHDLKGQGIYAYVTLMAGV
                     EPTDALRKELVAHVRKEIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAANECEGLG
                     DTSTLADPTVVDDLIDNRAVK"
     gene            complement(16271..>16575)
                     /locus_tag="PK144_03960"
     CDS             complement(16271..>16575)
                     /locus_tag="PK144_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNF64913.1"
                     /translation="RQIFLQQQLGLPQPAYLHLPLVTDARGDKLSKLTRAPPLPGGDP
                     RPALHEALAFLGQPQPPAGLSATELLERAVRAWDCNSLPASPGRTWAGTLTLATGP"
BASE COUNT         2450 a         5518 c         5893 g         2614 t
ORIGIN      
        1 gtctggtccg gtggctggcc atgcggggat tccggacgtg atgccgatct cgcgtgaatg
       61 caggtttttc tcctcctctc cccaaccctc tcccacgagg ggcgaggggc tgaaaagcga
      121 agtgttccgg tgtgatcggt atggctcaga tgccgaggat cttcttggcc ttgccgagcg
      181 aatcgaccgc gccggcgtga tccccggcct tgtgctgctc ctcgcctttg gcgcgcagtg
      241 cccggacctc ggcgagctgc tcggtgccga gcttcggtga cgcggcgagg gcggcgtcga
      301 tctgcttcat gtcgaccgga cagtgatagg cctgggccgg ggcggcgagg gcggcgagca
      361 ggacggcaac ggagaaggcg ggcaggtggc gcatggcggt actccggcag ggcgcggcac
      421 tgggagaagc ctgcgccggc cgcgcaggcg caggcagcgt gacgatagga cgcgccggcg
      481 ccattgccag cagcgacggc ctacaatgcg cgccccgtga ccgccacgat gctcccgatt
      541 cccccgatgc tgcgctccga tttccacttc gacctgccgc ccgaactgat cgcccagcag
      601 ccgccggccg agcgcggcga cagccgcctg ctggccctcg acggcacgga cggccggctg
      661 caggacgccc gcttcgacga tctgccgggc ttcctgcgtg ccggcgacct gctggtgttc
      721 aacgacacgc gcgtgatccc ggcgcggctg cacggcatca aggcgggcag cggcggacgc
      781 gtcgaggtgc tggtcgagcg catcctcgat gcgcggcgcg tgctcgcgca cctgcgcgcg
      841 tcgaaatcgc cgaagcccgg cacgcgcctc tggctggagc aggcggtcgg ggcggtcgtg
      901 ctcgggcgcg ccggcgaact ctatgaactc ggcttcgagg atgcccggcc ggtgccggcg
      961 atcctcgatg cgcacggcca catcccgctg ccgccgtaca tcgaccggcc cgacgcgccg
     1021 gccgacgccg agcgctacca gaccgtctat gcgcgcgccc cgggcgcggt cgccgcaccg
     1081 accgccggcc tgcacttcac gccggcgatg ctcgaacgcc tggccgcggc cggcatcggg
     1141 cgggcgttcg tgacgctgca cgtcggcgcc ggcaccttcc agccggtgcg caccgagcac
     1201 atcagcgagc accgcatgca cgccgaatcc gtcgaggtca gcgccggaac cgtggccgcc
     1261 gtcgccgcgg cacgggcgcg cggtgcgcgc gtggtcgccg tcggcaccac cgccgtgcgc
     1321 agcctcgaat cggcggcggc cggcggggag ctgcggccgt ttgccggcga caccgacatc
     1381 ttcatcacgc cgggctaccg tttccgggtg gtcgatgcgc tggtgaccaa cttccatctg
     1441 cccgaatcga cgctgctgat gctggtctcg gcgctggccg gccatgcgca ggtgatggcc
     1501 gcgtaccggc atgcggtggc ggcgcgctac cgcttcttca gctacggcga cgcgatgttc
     1561 gtgcgcggta gccggccagc gggctgaacc gttacgaagc tcttacaggt aacgccttgt
     1621 cggcgcctgc cggcgccccc atgatcgtcg cagtcccgcg cccgcagacc ggcgcgcggg
     1681 gatcgtcgag gagactcccc atgaaacgca tcctgctgtt tgtcctgacc aacatcgccg
     1741 tgctggtggt gctgagcatc acggcgagcg tgctcggcgt cgatcgcttc ctgacgcgcc
     1801 aggggctgaa cctgccggcg ctgctcggct tcgcggccgt gttcggcttc ggcggttcgc
     1861 tgatctcgct ggcgatgtcg aaatggatcg ccaagtcctc ggtcggcgcc tacgtgatcg
     1921 aggtgccgca gaatccgacc gaacgctggc tggtcgagac cgtgcagcgc caggcggcgg
     1981 cggccggcat cggcatgccc gaggtcgcga tctacgactc gcccgagatc aacgccttcg
     2041 cgaccggcgc gagccgcaac aacgcgctgg tggcggtcag caccgggctg ctgtcgaaca
     2101 tgagccccga cgaggtcgag ggcgtgctcg cgcacgaggt cagccacgtc gccaacggcg
     2161 acatggtgac gctggcgctg gtgcagggtg tggtcaacac cttcgtgatc ttcctgtcgc
     2221 gcgtggtcgg cttcgtggtc gaccgggcgc tgtccggcaa ccgcgatgac gagggcgggg
     2281 tcggcatcgg ttacttcgtg accagcctga tctgcgagat cctgttcggc ttcctggcct
     2341 cgatgatcgt catgtggttc tcgcgccggc gtgaattcag ggccgatgcc ggcggtgcac
     2401 acctcgccgg ccgcaaccgc atgatcgcgg cgctcgaacg cctgcgccag atgcacgagc
     2461 cttcgcacct gccctcgcag atggcggcct tcggcatcaa cggcggcacc gtgcagcgcc
     2521 tgttcatgac ccacccgccg ctggaagagc gcatcgccgc gctgcgcgcc gcctgagcgt
     2581 cctcgccccg cgcacgcccg gaagcccgcc gcctgcggcg ggcttttcgt cgggggcgga
     2641 taaggggagc ggaaaggcgc gccgggttca gccgaactgg ctgaagtcga acaccagctc
     2701 cggggccggg ggctgcacga tgtcgccctg gaccagcatg gcgccgaact gccaggtgat
     2761 cgccatctgc gccgggttgt cgacgccggt catgatcacc tgcgtgtgct gctcggcagc
     2821 gagcgccgtc aggtgatcga gctgctgctt gaaggcggca tcgcgctggg cctgctccat
     2881 gagttcgatg tcgatgcgca cgaaacccgg ctgcgactgg cgcagcagct gctccgagta
     2941 cgggcggccg ccgaagccgt cgagcgcgat gcggcagccg cgcggttcca cggcctgctt
     3001 gagctggatc aggtcggcga agtagggctc gacgacggtc tcggcgacct ccagcaccag
     3061 cacgctgccg ggcaggttgc gctcggcgag ctgctgcagc agccagtcgc ggtattcggc
     3121 atcggtgatc gagttcgacg acacgcgcac gaacacggtc ggcaggccgg caccggccgc
     3181 gccgcgcgcc gcgatcgccg cgagcgccgt cgccaccgtc cagcggtcga gctggcgcat
     3241 cagtccgcgc gtctcggcga gcggcagcgc ctcgcgcagg tcgagcggct ggccctgttc
     3301 gtcgctcgcg ctcaggcagg catcgaagaa ctcgccgcgg gcaccggtga agctcgcgat
     3361 cggctggtag tacagatgca cgcgctgttc gaccacggcc tgacggaagg cgcgcaggat
     3421 ggtgtcgcgc cgcgcgatcg agtcgcgctc cggggcgacc ggcgtgtaga tctgcacctg
     3481 actgccgccg agctggcggg cctgctcgca ggccttgtcg gcgtaggaaa tgatctgcgt
     3541 ggcgctggag ttggtgtcgc cgatcatgca gatgccgatg ctggccttgg cggtgatcag
     3601 catgccgccg gcctggatcg tgtggttctc gatgccctgg cgcacgcgct cgccgagttc
     3661 ggcgacggcc ccgcgcgccg acagcggcgt atgcaccgtg aaggttgcat ccgagaagcg
     3721 ggcaacgatc tcctgcggcc cgacctgctg ctggatcgcc tcggcgacgc cgcgcagcag
     3781 gtcgtcgacg gcttcgagcc cgaggcgttc ggcgacaccg cggtagtcgg tcatcagcac
     3841 gtagagcacg gcaccgccga ggctgccgct ggtggtcgcc gaggtgtgga tgcggttcag
     3901 cagctcggtg aagtactggc ggttgaacag cccggtcagc gtgtcgtgtt cgacgaagta
     3961 acgcagcttg tcctgcacct cgggctgctc gccgcgcggt tcgcgcacca gcagctgcag
     4021 gcagaattcc tcgttcatgc gcgctgccga ggcgagcagc acgatgcgga tctcgctgcc
     4081 gtcggcgcgc acgccgcggc attcgaggcc gtcgccgagc ggggcgttcg ggttgcgctc
     4141 gcgggcgcgc aggtagctct tgaccgcgtc gcggctgtcg cggtggatca ggtcgatcag
     4201 cgtggtgtcg accagcgcgt cgtcgaaacc gaacagcgcc cggtagctcg ggttggtgta
     4261 gatgtgcgcg ccggcgtgga tgtaggcgat cgcatcgcgc gaggtttcga gcagcgcctg
     4321 cgcgcgggcc tcggcctcgc ggtacaggcc ttcccagcgc cgcgcctggc ggcgttcgtt
     4381 gagccggccg agttcgagcc gggcgaccag cggcagcgag ttcgagtgat tcagcgccgc
     4441 gatgcggtcg gccccggcgt tgagcaactg cacggcgtcg atgccggcgg tctcgtcggc
     4501 gacggcgatg accgggatgt ccttgaaggc gctgtcgatc gagtcacgga cgttctcgat
     4561 cggcagcacg ccctcgccga tgcgcacgat ggcgaggtcg aaggtctgtt cgtcgatcag
     4621 gtgctgcagc gtgtcgatgc ggtcggcctg ccagtggcgc accaggaaac ccgccgcccg
     4681 cagcgggcgc agtaccggct gggcatcggc ctggggccgg tcgaccagca gcagacggat
     4741 cgtcgaatca agggacacgc gcggtttacc tcgggtcagg acgcgacgga tggcgctccg
     4801 tgcaggctgg aatcagcaga ggggagtctt ggcgggccgg ccggcttcct cgcgtaccag
     4861 ccggggcacc agataaccgg gcagcatcgc ccgcagtgcg cccatgatag acaaggcctg
     4921 ttgctcggaa acctcaaagt gtgtcgcacc ccccacgcga tcgagcaggt gcaggtaata
     4981 cggcagcacg ccggcggcga acaaggcctc ggacagcgcg gccagcgtcg tcgccgcatc
     5041 gttgacgccg gcgagcagca ccgactggtt cagcagcgtc acgcccgcgg cgcgcatgcg
     5101 tgcgcaggcc tgcaccagcg cggcatcgat ctcgcgggca tgattgacgt gcagcacgag
     5161 cacgaccggc aggcggctgc gcagcgcagc gagcaggccg gcgtcgatgc gcccggggtc
     5221 gacgaccggc tggcgggtat ggatgcgcag gcgttcgagt tgcggcacgg cgtcggcggc
     5281 ctgcaccagc tcacgcagcc ggcggttcga cagcgtcagc ggatcgccgc ccgacaggat
     5341 cagctcgcgg atcgaccggt cggcgtgcag gtgcgcgaag gccgcctccc agccggcggg
     5401 actggcgccg gtgtcggcat aggggtactc gcgccggaag cagtagcggc agtggatggc
     5461 gcaggcaccg gtcacgagca gcagcgcccg tccggcatac ttgtgcagca ggcccggcgc
     5521 gacgcgtgct gcggcatcgc cgaccggatc ggcgccgaag cccccggccg tggcgagctc
     5581 atcggcgagc ggcaggacct ggcgcagcag cggatcggcc ggatcgccgg gacgcatgcg
     5641 cgcgacgaat ccggccggta cccgcatcgg aaaccgttct gccgcgcggc gcgccgccgg
     5701 cagcagggcc gcatcgaggc cgagccgttc gagcagggtg gcgggatcgg tgagcagacg
     5761 ggccccggac ccgtcggtgg caggcgtgtc gtcggcatgc gcgacagcgg ctatcatcgt
     5821 cggccttctc aactcggata cgctccgttc cacgcaggtc agcgacgatg gcgacttata
     5881 gtaccaacga attcaagtcg ggtctgaaga tcatgctcga tggcgacccg tatgccatcg
     5941 tcgagaacga attcgtcaag cccggcaagg gccaggcctt caaccgcgtg cgcgtgcgca
     6001 acctgaaatc cgggcgcgtg atcgagcgca cgttcaagtc cggcgactcg gtcgagggcg
     6061 ccgacgtgct cgacatcgac atgcagtacc tgtactgcga cggcgagttc tggcacttca
     6121 tgaacccgac cagcttcgag cagctcgctg ccgatgccac ggccgtcggt gacaacgccc
     6181 ggtggctgaa ggaaaacgac ctctgccagc tgacgctgtg gaacggcgtg ccgctgtcgg
     6241 tgctgccgcc gaacttcatc atcgcccgca tcaccgagac cgacccgggc ctgaagggcg
     6301 acacctccgg cggcggcggc aagccggcga cgctcgaaac cggcgcggtc gtgcgcgtgc
     6361 cgctgttcgt gcagaccgac gaggtgatca aggtcgacac gcgcaccggc gaatacgttt
     6421 cgcgcgtcaa ggaatgaacc gtccttgccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nccgttgtcc ggcccggctg actggcggcc gtcatgtgcg cccgaagccc
     6601 tgcgcctgcg cgccgggctg ctcgcgcgga tgcgcgcctt tttcgaggcg cgcggcgtgc
     6661 tcgaagtcga caccccggcg ctgtcgcggg cggcggtgac cgatccggcg ctggcgagct
     6721 tcgccgtcga ctggcacggc ccggagcccg ggcggctgtg gctgcagact tcgccggagt
     6781 acgcgatgaa gcggctgctc gccgccggca gcggcccgat ctggcagctc gccaaggtgt
     6841 ttcgcgatgg cgaggccggt cgccggcaca accccgaatt ctcgctgctc gaatggtacc
     6901 ggcccggttt tgatcatcac gcgctgatgg acgaggtcat cgacctcgtc agcgagctgc
     6961 tcgccggcag gcgtgcgctc gccgagcccg agcgcatccg ctatcaggac gctttcctgc
     7021 agcacgccgg cgttgatccg ttcgctgcgg acgtgccggc gctgcgggtg gcctgcaccc
     7081 ggcacgcgct ggtaccgccg cccggcatgc cggaagcgca ggtcgaaccg tggctggacc
     7141 tgctgctcag tcacgtgatc gagccgcggc tcggctgcgg gcggctgagc ttcctgtacg
     7201 actatccgcc ggcgcaggcc gcgcttgcgt gcatccgccg tgacgagacg ccggccgtcg
     7261 gcagccgttt cgagctgtat ctggacggcg tcgaactggc caacggcttc cacgaactcg
     7321 gcgatgccgg cgagcagcgc gcccgtttcg aggccgacaa tcgcgcccgt gtgcgggccg
     7381 ccctgccggt gatgccgatc gacgaagcgc tgctggcggc gctggccagc ggtctgccgg
     7441 cctgcgccgg cgtcgcgctc ggcttcgacc ggctgctgat gctcgcggcc aatgccggcg
     7501 ggctggatgc ggtgctggcc tttccggtcg atcgcgcctg agcggggccg gaaccgcggc
     7561 gtgcgctccg gccgggggaa ccccggagga catgaaaaaa gcccggctgc acgagccggg
     7621 ccggatgctg ctgcgccggg ggagggcggg acgtgcgatc aggcggccga agcggcgcgg
     7681 cgagcgacct tgacgatcgg cttcggcgcg aagtcggtgc tgttggcggc gatcagctgt
     7741 tcgaactcgg ccttgaagcc ggcgagcagg tcggcgtagg cctgggcgtc gtcgagcgcc
     7801 ttctggcgca gcgcgccggc ggcttcgagc tgggcggccg agaaggcctt caggctctcg
     7861 acatcggtca cgtcgctggc ggcctgcagg cggccgaggc cgaagccggc ataggagcgc
     7921 agcagcccga actggaaggc ggcgagcttt tcgaggctgg cgacgaacag cttgttggcc
     7981 tgcaccaccg gcgcgatcca ggtggcggaa cgggcaaggg cttcgttgaa cgggtcgagg
     8041 tcgtacatgg cgggcatccc ggcggaagtg aaggaataag gtgaaggaag tttctgttgc
     8101 gatgcagcat aacagcgtgg atgttgcgtc gcaacaatca agacccgtgc cgttttcgca
     8161 tcacgcaaaa tcaccggacg cgccgccggg ggccgcggcg gtttgaagct ccggcaggct
     8221 gtgccaaact cgcgcggttt tggcggaccc ccgtccacgc cgcgacacgc gtctcgccgt
     8281 ggctgcggcg caggcatcag cagaaccaac gcagagcggt acatggacaa gcgagtggaa
     8341 accctcctct ccctggaaat gccgtaccgg gagcggatga ggctgcagcg cagcgtgttc
     8401 cggggcggtg acgggccgcg ggtcgccgtc gtcgccggca tccatggtga cgaactggaa
     8461 gggctgtatc tgtgtcaccg gctcgccgcc tggctcgatg cgctcgccga gcgccggccc
     8521 ggggcgctgc tcggcaccat cgaactttat ccggcgctga atccgctcgg tctcgacacg
     8581 ctggcgcgca cggtgccgcc gtacggcgtc gacctgaacc ggtccttccc cgggcacgcg
     8641 cggggcgtgc tgccgcagcg catcgccgca gcgacgatga cggcactcgc cggctgcgcg
     8701 ctggtcgtcg acgtgcatgc cagcaacatc tacctgcgcg agattccgca ggtgcgcatc
     8761 aaccatgtgt tcgccgaatc cctgctgccg tacgcgcggc gcatgaatct cgacgtgatc
     8821 tggctgcacg gcgccgtgac cgtgctcgaa gccaccattg cccatagcct caacgaggcc
     8881 ggcgtgccgg cgctggtggt cgagatgggc gtaggcatgc gcctgacgcc ggtctacacc
     8941 gagcagctga tcaccggcct gctgaacgtc tggcaggggc tcggcgtgct cgccgccgac
     9001 ctcgaactgg cgccggtcac gcacgagccc cggctcgcac acgaggacaa cgtccactac
     9061 ctgaacgctg cgacctccgg cctgttcgtg cccgaggtcg agcactggaa ggccgtgcgc
     9121 gccggcgggc tgctcggcca catcgtctcg ccgatggaag gccggcggct ggccgaggtc
     9181 cgctcgccct gcgatggcgt gctgttcacg ctgcgcgagt acccgctggt ctacgaaggc
     9241 tcgctgatgg cgcgcgtggt tgccgatgcg gaacccgaaa acggagaaag cgcatgaaca
     9301 cgccgacgct gctgcgcatg acggcgccgc tgcgggatga cttcgacatc ccgtaccacg
     9361 acgtcgggcc ggtcgagcgc acgccgcggc tggcactggt ggcggggctg cacggcaacg
     9421 agctgaacgg cgtgttcgtg ctcgcgcggc tcgcggcctt cctgcgcgat gtcgagcagg
     9481 gcaagcgtcc ggacctgcgg ctgcgcgagc gcgtgctgct ggtgccggcg gtcaacgtgc
     9541 tcggcgtgaa cctgctgtcg cgcgtctggc cgttcgacaa gatcgacctg aaccgcgtgt
     9601 ttcccggcct cgaaggcggc gagaccacgc agcgcatcgc cgccgcgctg ctgcgcctga
     9661 cgcgcggcgc ctattaccgc atcgacatcc acagctccaa cctcgacatc gaggagctgc
     9721 cgcaggtgcg cttgtacgcg ccgaatgacg acgagcgcgc gaccgcctgc ctgttcggcc
     9781 tgccggcggt catcgagcgg ccgctgaaca agatgttcat ggcgacgctg gtccacgcct
     9841 ggcgcgcgca cggtggcgag aacttcgtgc tgcaggccgg gcgggccggt gacctgcagt
     9901 cgcgccactg cgagacgctg ttccgttcgc tggtggcgtt tctggaccgc accggcatcg
     9961 tcgagggtct gacgctcgcc gatgacgagg aggacctgca ctacttcggc ctgcgccagt
    10021 cctatgcggt gctctcggag caggccggct tcttcgtcgg acgcattgat cccgggcgct
    10081 gggtgcaggc cggcgagctg ctcggtcacc tgtacgacgg ctttaccggc gatctgctga
    10141 aggacgtgca cacgccggtg gccgggctgg tctgcagcgt gcgccgccag ccgctgctgt
    10201 tcgagggcga tctgctctgc cgcatccaga cgctcgacga ttccggcaac gacgtcgaag
    10261 tcatgctgca cggccagggc cagtgaaccc gcctgtcccc cccgaatccc gaacgccaga
    10321 cgaagccacc atgtccgaaa tcgacaccgc cacccgtacc gagctcgaag ccgccgcctt
    10381 ccgccggctg gtcgagcacc tgcgcgagcg cagcgacgtg cagaacatcg acctgatgaa
    10441 cctcgccggc ttctgccgca actgcctgtc gaagtggtat ctggccgcgg cgcaggcacg
    10501 cggactcgct ctcgactacg aaggcgcccg cgagatcgtc tacggcatgc cttacgagca
    10561 gtggaaaacc ctgcaccagc gcgaagccag ccccgaacag cagaccgcct tcgccgaggc
    10621 gatccgcaag gtgcatgact gaaggtgcat gactgaaaga gcggggcggc ccggtgcgcg
    10681 tcagtactgg cggttcgcga gtttcaggaa cagcgcgctg acgcgttcgc gttcccgggg
    10741 cggaaacgcc agatagcgcg tgccgcccag ttccagcgcc atcgagccgt cggtcgcatc
    10801 gagcacgccg ttcaggccga tccattcggt cggctcgtcg gcgccgcgca gcagcagcgt
    10861 cagcaccaga tggccggacg gcagcaggtt cacgtcctgc agttcggctg attcggcatc
    10921 ctcggtgccc gggcattgcc agtcacagcc tgccagtcca tcgacgaaca cgccgatgat
    10981 cggccgtgga ttgaagaatt cgactacgtt gcgcacgggt gtcgagcctg tggtgtcgga
    11041 cggctcgtcg agcgttgcgc tggaacggcc gtcctgtcgg ttgtaggtcc atcggtggta
    11101 cgcggcaacg ggcatgccgc gctccggaaa aggtgccagt gatcatgagt atgagcaagc
    11161 cggtcaggcc gggtggcggc aggcgggtgg gcctgatgct cgtcatcgtc gtgctggcga
    11221 cagcgctctg gtatggatgg caggtgccgg agctgcgcca ccggctcatc tacggcttcg
    11281 agcactgggt cgcgggcctg cagacgcgtt tcaacatccg tccgccgggt gccgagcccg
    11341 agcccacggc cggcgaggtc gagttgctga aacccccggc ggcactgcgt ccgggcactg
    11401 ccccggccgc ggcggcgcag gccccggccg cccaaggtgt ccgcgatccg tccatcaccg
    11461 acgacgaggc gctgctgtcg ggcgcgcagc cgatgccgac gctggcggct ccgcgctcgc
    11521 tgaagttcgc cgtcggcgag gaggcggtgt tcgggctgga actgctgccc ggcatcgctg
    11581 cgcgcgggca gcgcgtcatc atcggcggac tgccgatgga tgccagcctg agcgccggtg
    11641 cccgcgacga gatggggcgc tgggtgctgg atgccgtcgc cctgccgggg ctgaagctgc
    11701 gcagcggcgc accgggtcat tacaccctgc tgatccgcac gcagccgctg cagcgcctgc
    11761 gcgtgccccg gccggtcgcc gaggccgcgg tcgaggtcac ggtgctgccg gcggcagggg
    11821 ccgaggcccg gaccgcggcg cccccggccg cgaaaccgcc gcagcccggt cccggcgaac
    11881 tgctcgatcg cggacgccgc gcgctggaac ttggtgacct gggcgcggcg cgtctgtatc
    11941 tggaagccgc ggccgggcag aacagcgccg atgccgcgct gctgctcgcg gccagctacg
    12001 atccgggccg cagcggcgac aacgccgatg tcggactcgc cgcggactgg tatctgcggg
    12061 cacgcaagct cggtgctgcc gaggcccagc ccgccatcga tgcgctgcgc agctggctgc
    12121 aggcccgcac cgagcccggc gaggccgagc gcaaggcgct ggcgaagctg tccgagccgg
    12181 caccgggccg ctgacacggc ccggacaagc ggggcgtccg gcgccgtggc ggacgcagtt
    12241 tcagcgttcg gactcttcca gtgtgccgca gtcgggtcgc cggccgctca ggcgcgcggc
    12301 atcgaaggcc gttgtgaccg cggccgcagg cgtgtgcatc gcctggatgc ggctccgatc
    12361 gatcggatgt accagcagca tcggcggtgg gcgttcaccg gaggtgcctt cacgcacgcg
    12421 ataggcctgc agctgcgccc aggccagtga cgcggcggct ggatcgaaac cggctgcgcc
    12481 catcagggtc agcgcccgac cgtcggcggc ggcttcctcg ctggcggaat acgcaaaacc
    12541 cgccccgacc tgggacgcac cgccgatggc cgcgatggcc tcgttcgaca gcgcggcatc
    12601 gccttcgagg ctggcggcga ccgagggggt gatgtgcgca ccggtatgtc cgagcaattc
    12661 atgggcgatg ccgtgggcga gcacggcggc gagccggtcc tgattgccgg tgacgcgcag
    12721 cagcgccttg gtgacgccga gccggcggcc ggcgagtgcc caggcatccg gcgtctcgtc
    12781 gcgcatcacc acgacctccc agtcggccgg tgctgccggg atcgcctgga tcagggcatc
    12841 ggccacgcag cgcacgtagc gtgtggtgtc gagttcgcgc agcagttcgc cgcgacgctg
    12901 gatctgcagg ctcgcccgcg ccccggccga ggccaggcgt tcggggggca cctggacggt
    12961 gttctgcggg ccttccgggg ccgcggcgca gccgctcagc agcagcagca ggacgacagc
    13021 gcggggcgtc ggtaacggcg ggtacggcat cggggagtct ccacgggcgc cggggcggcg
    13081 gcgcgcgcga agattacgca agccgcgcgc gagccgtcac gtgcccgtgt gcaagcaacg
    13141 ggcgggttcc aggctcagtc gagggcgcgg aacggatcat cgcggtcgcg gccgtcgccg
    13201 gtcggcaggt cgctgtaacc gaggtcctcg ccgtcgacat cgtcgaaggc gccggcccag
    13261 tcatcggggg cctcgatgcc ttcgagcacc agttcatacc aggcgtcgag ttcgatctcc
    13321 tcgacctcgc cttcctgaaa ctggacctcg acggtgcgtg catcctcgtc gaaggcgacg
    13381 acctcgaagc aggcgtggct ttcgaggtcg cggtaccagt tgccgacgat cggatccatg
    13441 tcgctcatcg cagtgttctc cgcttggcta cggaccggcc aagcatggcc tgccgtgacc
    13501 ggcttgcaag cctgcaggag gccggaaaag ccgcaggccc ggccccgcga cgagcggaac
    13561 cgggcctgcg ggatcagccg gtgcggctta cttgaccgcg cggttgtcga tcaggtcgtc
    13621 gacgacggtc ggatcggcca gcgtcgaggt gtcgcccaga ccctcgcact cgttcgcggc
    13681 gatcttgcgc aggatgcggc gcatgatctt gccggagcgg gtcttcggca ggccaggggc
    13741 ccactgaatg acgtccggcg tcgcgatcgg gccgatctcc ttgcgcacgt gcgcaacgag
    13801 ttccttgcgc agcgcgtcgg tcggctcgac accggccatc agcgtcacgt aggcgtagat
    13861 gccctgaccc ttgaggtcgt gcggatagcc gaccaccgcc gcctcggcga ccgccgcgtg
    13921 cagcaccagc gccgattcga cctcggccgt gcccatgcgg tggccggaga cgttgatgac
    13981 gtcgtcgacg cggccggtga tccagtagta gccgtcctcg tcgcggcggg cgccgtcacc
    14041 ggtgaagtac gtgccggggt agaccgacag gtaggactcc acgtagcggt tgtggtcgcc
    14101 gaagatcgtg cgcatctggc cgggccagga ggccttgatg accaggttgc cctcggtcgc
    14161 gccttcgagt tccttgccct cggcatcgac cagtgccggc tgcacgccga agaacggccg
    14221 tgtcgccgag cccggcttca gcgccgtcgc gcccggcagc ggggtgatca ggatgccgcc
    14281 ggtctcggtc tgccaccagg tgtcgacgat cgggcagcgg ttgtcgccga ccacgcggta
    14341 gtaccactcc caggcctcgg ggttgatcgg ctcgccgacc gaaccgagca ggcgcagcga
    14401 ggcgcggctg gtggccttga cctgctcctc gccgtcgcgc atcagcgagc ggatcgcggt
    14461 cggggcggtg tagaagatgt tgacctggtg cttgtcgacc acgtcccaga agcgggtcga
    14521 ggtcggatag gtcggcacgc cttcgaacat cagcgtggtc gcgccgttgg cgagcggacc
    14581 gtagacgatg tagctgtggc cggtgaccca gcccacgtcg gccgtgcacc agtagatgtc
    14641 gccgtcgtgg tagtcgaaga tgtacttgtg cgtgatcgag gcatacagca ggtagccgcc
    14701 ggtcgtgtgc tgcacgccct tcggcttgcc ggtcgagccc gaggtgtaca ggatgaacag
    14761 cgggtcttcc gcgtccatct cctcggccgg gcagtcggcc ggggcggcct cgacgagctc
    14821 gtggtaccag acgtcacggc cttcggtcca ggcgatcttg ccgccggtac gcttgacgac
    14881 gacgacgttc ttgacgcact cggccgccgc cgccgccttg tcggcgttgg ccttcagcgg
    14941 cacggcacgg ccgccgcgca tcgattcgtc ggaggtgatc acgacggcgc agctggcgtc
    15001 gacgatgcgg tccttcagcg cgtcgggcga gaagccgccg aacacgatcg agtgcacggc
    15061 accgatgcgc gtgcaggcga gcatcgcgac ggccgcctcg gggatcatcg gcaggtagat
    15121 gcagacgcgg tcgcccttct ggacgccgag gcccttcagc gcgttcgcga agcgcgagac
    15181 ttcctcgtgc agctgcttgt aggtgatctt gcggtcttcg ttcgggctgt cgccttccca
    15241 gatgatcgcg acctggtcac cgcgggtttc gaggtgacgg tcgaggcagt tgtagctgac
    15301 gttgagcttg ccgccttcga gccacttgat gcggccggcc ttgaaatcgc agtcgctgac
    15361 cttgctccag ggcttgaacc aggtcaggag ttcaccggcc ttctcgcccc agaaaccatc
    15421 ggggtcgctg atcgagcgcg cgtacatttc ctggtactgg gcgtcgttga tccaggcttt
    15481 ggccgccact tcggcgggga cgggatagac cttttcttcg gacatacaag ctactcctct
    15541 gatcaacagc cggctcccgc gcacgggagc gcccccaaga ttcactgaca ccgtccgcgg
    15601 gggcgggcga tgccggtttc aagctgcggc agcctgcacg ggcaggccgc gcactcgttc
    15661 actgcaggtg tcaggctgac accggaaagc gcggcgaccg gccatggtcg accgcgcgac
    15721 ccctccgcgc gggacggggc gccacagcgg cattcgccgg atcgggctga tggttggaaa
    15781 tgcggctgac cgcagtgcag caaaatcggc gggaacagaa cataatccgg acggcgtgcg
    15841 gtgaatgcga gcgtgcgatt cgcaagtacc gtatcaagcg cctcatcccc ttggtaatgg
    15901 tcccgggcga gcgcccggaa agcacggctt attgttttat tgttcgattg cgcgtggcgc
    15961 gatctgcatt tttccctacc gctcccggca aagtcaacgg ctgtgtcggg tgccggcccg
    16021 tcagggcttt tgtctgcgac aaggctctga tgaaaaaggc gaagttcctg gaatccgcct
    16081 gcagggcagg agaggttgat cctaagtatt gttccttttt ttataagggt ttatacctat
    16141 attatttgcg atgcgatatt ataatttcaa accatgaaaa attagcagtt gcgataattc
    16201 tgcaggcggc cattgcagtt gacaggggcc gggctggaac attgcctgtt ggtaatgctg
    16261 caatgcgcaa ttaggggccg gtggccagcg tcagcgtgcc tgcccacgtg cggccggggc
    16321 tggcgggcag ggaattgcaa tcccaggccc gtactgcccg ctccaggagt tccgtggctg
    16381 acaggcccgc cgggggctgc ggctggccga gaaaggccag cgcctcgtgc agcgccgggc
    16441 gcgggtcgcc ccccggcagg ggcggtgcgc gggtgagttt ggagagcttg tcaccccgcg
    16501 catcggtgac cagcggcaga tgcagatacg ccggctgcgg caggcccagt tgctgctgca
    16561 ggaagatctg ccggg
//
LOCUS       DAOQIE010000037        16427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1654_length_16427_cov_6.986257, whole genome shotgun sequence.
ACCESSION   DAOQIE010000037 DAOQIE010000000
VERSION     DAOQIE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16427
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1654_length_16427_cov_6.986257"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2925)
                     /locus_tag="PK144_03965"
     CDS             complement(<1..2925)
                     /locus_tag="PK144_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNF64914.1"
                     /translation="MSDSFPFDQAATATLERILERLTEDGHDFTDYKSGTLRRQVLRC
                     AGGRTQAALSAYEARLAADPIERRRLAESFSIGVTHFFRDRRAFSALASALETTTRRL
                     PDGHTLRVWVPACCTGEEAYSLALMLSGLRTGSGQPLDWKIFATDIDARAIAIARRGR
                     YATEAVRRRVPADILARHFTEVPGGYVIAPAIRARMVFARHDVLSAPPFLNLDLVTCR
                     NLFIYLRPAAQERLLAVFHAALGSEGLLLLGRRESIRTESPLFTPLDGSARLFVRRPA
                     RAHPPTGARCTPTLPTLPAAARNRLGLYGAVITALAPNAVVIDETGNCLHFFGEVRSF
                     LGFVRNEPDLRLANLLPEPWRPAYQVALSRARRATVKDGIDSVDLPQITGYGSATGRW
                     QLSIRPLHGEELGHGWLVSFEAQSVLAKDPAHNSGIEGNIGFESELDDLNRRYQEALA
                     AAATVNDELQATNEEMQAANEELEATSEEMQAANEELESTNTELEIRLNELAVLNAEL
                     NEILGSAGQMQIVLDADLRVCRFSPSATRLYALDQNDIGRPLALVQPIEPLPGLDARL
                     RRVLSTGLPLMEEIDAGDRALMLQITPVGKSAATRRLILGATDTTALRSARRAQQIGE
                     ERLRESENRFQRIVEAIPVGIAQHDPNGDVRFLNPYFTQLFGYSIEDIPTVEDWYRLA
                     WPDADARARARQQWQEDLRAQRLGLDTPAREQQIRCRDGNLRSVEIALARGGSGPYDG
                     IYAVFSDVTEQREVMQRLEQAMLAAQSAARAKSEFLANMSHEIRTPMNAIIGLSELAL
                     HGELPPRQHQYVQRIHQAGVSLLGVVNDILDFSKIEAGKLSIEQAPFSLGRLLRDVLD
                     LVAGNAHTRGLYLYASVDPRVPDALTGDALRLQQVLLNLLGNAVKFTPQGGIVLSIRP
                     GGSPRATTLQREFVFEVTDTGIGIEAEERQRLFQPFEQSDPSTTRRYGGTGLGLTICR
                     RLA"
     gene            complement(3067..3972)
                     /locus_tag="PK144_03970"
     CDS             complement(3067..3972)
                     /locus_tag="PK144_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF64915.1"
                     /translation="MNLAADDLLLYARIADAGSFSRAAAALGLPKSTVSRRLAAFETT
                     LGERLLTRTTRRLVLTEFGEALLEHAHRLAEEMEAAVQLAERRKAVPQGRLRVTMPPD
                     LAVLMLVPMLARFGAGYPAVSVELDLSPHQVDLIAGGFDLAVRMGPLAEDASLVARRL
                     GSFARGLYASPDYLARRGTPQAPEQLAGHDALLLAPQAGRPLPMSLQNGLQEWHGPWA
                     SRFGANSVELLVRMALAGAGLVAIDERFVRPHLAAGTLLPVLPAWTLPPVTAWAVMPG
                     RRLMPAKTRAFVEALIRTLADDKGD"
     gene            4120..4794
                     /locus_tag="PK144_03975"
     CDS             4120..4794
                     /locus_tag="PK144_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNF64916.1"
                     /translation="MSQNQHPNANPTANPTVTRILQINASIQLDASQSTRLADAIVAR
                     LQQAHPQAVVQRRDLGRAPVPALDAAALAALFTPEEARTPEQAARVAADDALIAELQA
                     ADVIVLGVPMYNFGMPAQLKAWLDAVTRARVTFRYTEQGPVGLLTGKRVYVAHARGGR
                     YRGTALDHVTPQLDTHLGFIGLGDVRHVYAEGFAMGDEARAAGLADAARQLAALFDEA
                     ADAVAA"
     gene            4923..5822
                     /locus_tag="PK144_03980"
     CDS             4923..5822
                     /locus_tag="PK144_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF64917.1"
                     /translation="MNLPAADSPVIRHPRRVEGIVAGQATSDGAGVRLVRLLGAELQR
                     RLDPWLMVDAFSSDRPDDYLAGFPSHPHRGFETVTVMHAGRMRHRDSAGHEGLLRGGD
                     VQWMIAGRGLVHSEMPEQEDGLMAGYQLWLNLPAADKLRAPAWRDIPAAAIPQVRTAA
                     GVQVRVIAGASHGVEGAVQRPHTAPLLLDLSLPAGSRFAQPLPAAHHGFVIVRRGQLA
                     IGSRTVTAGRLALLAQEADSDGVVLNAGQEDVEALLVAGRPLGEPIVQHGPFVMNTRA
                     EILAAFEDYARGSFDTVSPQPFD"
     gene            complement(5774..8884)
                     /locus_tag="PK144_03985"
     CDS             complement(5774..8884)
                     /locus_tag="PK144_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64918.1"
                     /translation="MMRESEASRLLFLIRMLLPLLLLTLAVMLYQAYTDFRSTLLDRA
                     GQQMRIDAVAGASQFAELLREYRESLKWLAVRSARLDATDTPPDMLAALLDHYRFIHA
                     GTVVAIERRAADARLDAHAGLSLPPPAQDDTAAALLLQLAGRTVLVLTEPLTSAAGET
                     ARLRLALDLTTLASRALGALTKRDDVEGWLFDAEGRVLWQAHPAGAVPAAAASSPTAD
                     DFGVLRERLRRETAGSGLFRLQVWRDAQAHARDQLVGFAALPAEAGGLVLASAVEYRH
                     IAAPLDQFALQLLLIGGVLTLTLAATAALLQRFRASAGRRQQRLDRLQALIGRVDQAM
                     FDCEDPDRLHDSLARTLAEPVPGVQTALATRAADLADPLARERAQQVLSGGAARWDEN
                     RDGSVDALLPVSAPDGQPVAVLWFRQLGTPPPDAASRRLITRIAQDISHALVRMEDRS
                     RRNAAETDLRLVASVFENIREAAVITDADNRILRVNPAFTTITGYQPSEVIGQTPALL
                     ASGRHGREFYTAMWRSLQDTGSWSGEICNRRKDGCEYLEWLSISVVRDAAGVIMHHVA
                     VFADLSEHTAMRARIDWLASHDALTGLPNRASFIAALPGLLQRMAPEHCCAVLNLDLR
                     RFQRLNETLGRSGGDRVLQALAERLNALAARPACLLARIAGDEFALAATGLPDEQAAG
                     RFVAGLHTRLAEPLPVLPEPVSVSVDIGYALAPRDGRDGETLLRHAVAAARQARVSGE
                     GWFGFDPQLGQRMQRRALLERSLRTAISGGELSLVYQPQIALADERVIGVEALMRWNH
                     PRLGAVSPAEFIPIAEETGLIRTLGAWALDTACHEAAGWQRHCPRPPRVAVNLSARQF
                     TDPGLITGITHALHSSGLPPAQLEIEITESLLMDNPARTVGVLMALRRTGVGVAIDDF
                     GTGYSSLSYLKTFPLDRLKIDRAFVRDLPDNSDSAAIVQAIIALAHTLGLSVIAEGVE
                     DEAQLEFLLGQDCDEVQGYLFAPPRAPAELHALLARGTTHPPSAGRRQAQSKGCGETV
                     SNEPRA"
     gene            9316..10038
                     /locus_tag="PK144_03990"
     CDS             9316..10038
                     /locus_tag="PK144_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF64919.1"
                     /translation="MSAADLMAAAQRLSAALAPLHFRAPVTHVYNPLDYAGLTYGQYL
                     QRWGDGRRRVLFLGMNPGPFGMVQTGVPFGEIHAVRDWLGIDAPIGRPAVEHPKRPVE
                     GWACRRSEVSGRRLWGLFAGRFGTPQAFFAGHFVLNWCPLAFLADGRNLTPDRLPSDE
                     FAPLAAACDRHLREACAALEPEWLIGVGRFARLRAQQALTGPAAPRIGEILHPSPASP
                     AANRGWAAAASAQLQELGVWGA"
     gene            10113..11648
                     /locus_tag="PK144_03995"
     CDS             10113..11648
                     /locus_tag="PK144_03995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNF64920.1"
                     /translation="MSQPMMSATSELPSNVVAEARLPAVLLAIVLGWAALIGALAYFT
                     ERDIAGDRAQTLETAAQRAETLLPLFGNSVQRALDDTRLLLSALELALESNGGDGAAR
                     DLLQRFGAPQDGMRQRISIADARGRLIASTADLPAASLSVADRDYFQRLAAPDAPGWI
                     LGKPVISHVTDDWTIPLAVRRVAADGRFTGALITAIDPQWLLTMAGSMDLGRTGTVGV
                     VDFDGTVYLRRDIDLHHAGAASTIGTTNPRLIVLEPARTAPSGRFSGTSALDGLRRLG
                     SYRVLPQYGLIVAYGLGEDEVLTQHMRRAGAERRLALLSGVLLTAIAGGLFLWRLRDW
                     RQARQLLAAWRQAHTAARTDVLTGLGNRRGYEEHIGALLASRPPMPSSLLYIDCDDFK
                     RINDSAGHEAGDAVLCALAASFSDSVRASDYVARLGGDEFVVLLANAGEVAAAAIAGK
                     LKSRAETAVAAIGLPVTLSIGQITFRRVHDAAALTREVDAAMYSAKREGKNRIVRVTS
                     IGT"
     gene            11730..12872
                     /locus_tag="PK144_04000"
     CDS             11730..12872
                     /locus_tag="PK144_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF64921.1"
                     /translation="MTTYPMPNRNLPARPAPWLLLVLCLLGGLGACGERRAEPAAAAA
                     TAAAPIQVGPENLWTVVRARVQSGPLLTGTLRPERRAEVRAEIGGTLREVSFEPGQAV
                     AAGAVLARLDERAVRDARDSALSAVGSAERAALLARRNAERGDELLRTGAWTRRQQED
                     AWAASITADATLADARARLAAADKQLAYTVVHAPMAGRVAERPVAAGDVVPAGATLYV
                     LVDPASMRLEAAVPAAELPRVQAGAAVEFTVRGYPDRRFRGTIERINPMADPVTRQVT
                     LYVALPNADGALLGGLYAEGRVAADVREAPVLPIDAVPAGAAQASLWRVREGRLESVT
                     VETGLRDAEAGYVEIRSGLEVGDRILLRPARTLQAGTPVEILPVLR"
     gene            12908..16003
                     /locus_tag="PK144_04005"
     CDS             12908..16003
                     /locus_tag="PK144_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF64922.1"
                     /translation="MRISDFSIEQPMVTVVAMLALVLFGLFALLALHTDEFPDIEQPV
                     IVVGIPYPGASPEGVEREILERVEESITGIAGIHELRSSASDGYAQLIVFFEFGKNML
                     EAGQEIRDRISAVRDRLPDEMKEPVLTRVDPADIPVVSLTLASDRLTPVELTRLADPG
                     IVRELRQVPGVAQVDVAGGQTRELNVQLDPAALAEAGVTLAELVQALNAQNLAAPVGR
                     LTHGAGEQTLRLRGRLDGPEAFAQLVVARRGERVIRLGQLAHVSDGAEEARSLAFYDG
                     RPAIGIGVLKSRGASTTAVSAALRAQVAQVQATLPAGVDLRIVQDAGERVGHSVANVG
                     EHLVLGALLTVLTVFLFLNSWRSTVITGLALPVSVLAAFVAVWACGFTLNQMSLLGLS
                     LAIGILIDDAIVVRENIVRHVHLGRSHLDAARAGTAEIGLAVTATTFSIVAVFVPVAF
                     MSGVAGQWFKPFALTIACAVLVSLFVSFSLDPMLSAYWPDPQPEAQRRGWLTRALDRF
                     NAGFDRLTGRYVRVIGWALDHRLVMTALALGSFFGALWLQAQFGGAAFVPPSDRSELR
                     IVIDTPPGSSLEHTRVLAERVAGLARALPEVRYTYTRAGSAGDFTVGGVDTATVYVRL
                     KPKSGRSRTQEQIGNALRAQAQQLAGAEVAVFSGGFGGAYKPIQLRLIGPDARELAET
                     AQRLLAAAREVPGIVDANLSTRGRKPEYELTVDRGLAGALGITVADIGQGLRVAYAGL
                     EAGDWIDPDGETRDVTVRLAPEYRELAAGLARLPVAAHAADGRTVSLPLGQIVTVTPG
                     LGPRRIDHFDTERVIVIEAATQGVPLAEAVRRLDARLAREPLPPGLRLQHGGEAKDQA
                     EVFGNIFSALGIALLLMYLILVLQFGSFLDPVPIMASLPLSLIGVVLALLVTGDTLNI
                     MSLIGVMLLMGVVAKNAILLIDFARQGRARGLALREALIEAGRVRLRPILMTTFALTA
                     GMIPVALGHGEGADFRAPLGRAVIGGTLTATLLTLLVIPTFYELTDEWRTRVQGWCRR
                     R"
     gene            complement(16227..>16427)
                     /locus_tag="PK144_04010"
     CDS             complement(16227..>16427)
                     /locus_tag="PK144_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64923.1"
                     /translation="ASYAAITAGRIMRLRDRSAKRSQARRRAQTLLHRKNSLQQETAG
                     SAAQPGICPESPDFGRKRPSSA"
BASE COUNT         2250 a         5764 c         5990 g         2423 t
ORIGIN      
        1 cgccagccgg cggcagatcg tgagcccgag gccggtgccg ccgtagcgcc gggtcgtcga
       61 ggggtcggac tgttcgaagg gctggaacag gcgctggcgc tcctccgctt cgatgccgat
      121 gccggtgtcg gtgacctcga acacgaactc gcgctgcagc gtggtcgccc gcgggctgcc
      181 gcccggccgg atcgacagca cgatgccgcc ctgcggcgtg aacttgacgg cattgccgag
      241 caggttcagc agcacctgct gcaggcgcag cgcatcgccg gtcagggcat cgggtacgcg
      301 cggatcgacg ctggcgtaca ggtacagccc gcgcgtgtgg gcgttgccgg cgaccagatc
      361 gagcacgtcg cgcagcagcc ggccgagcga gaacggtgcc tgctcgatcg acagcttgcc
      421 ggcctcgatc ttcgagaagt cgaggatgtc gttgacgacg ccgagcagcg acacgccagc
      481 ctgatggatg cgctgcacgt actggtgctg gcgcggcggc agctcgccgt gcagcgcgag
      541 ttccgacagg ccgatgatgg cgttcatcgg cgtgcggatc tcgtggctca tgttggcgag
      601 gaattcggac ttggcccgcg cggccgactg cgcggccagc atcgcctgtt cgaggcgctg
      661 catgacctcg cgctgctcgg tgacatcgct gaacaccgca tagatgccgt cgtacggccc
      721 gctgccgccc cgtgcgagcg caatctcgac gctgcgcagg ttgccgtcgc ggcagcggat
      781 ctgctgctcg cgggccggag tgtcgaggcc gaggcgttgc gcgcgcaggt cctcctgcca
      841 ctgctggcgg gccctggccc gcgcgtcggc gtccggccat gcgagccggt accagtcctc
      901 gacggtgggg atgtcctcga tcgagtagcc gaacagctgc gtgaaatacg ggttcaggaa
      961 gcgcacgtcg ccattgggat cgtgctgcgc gatgccgacc gggatcgcct cgacgatgcg
     1021 ctggaagcgg ttctcgctct cgcgcaggcg ctcctcgccg atctgctggg cgcgccgggc
     1081 actgcgcagc gccgtcgtgt cggtggcacc gaggatcagc cggcgcgtgg ccgcgctctt
     1141 gccgaccggg gtgatctgca gcatcagcgc gcggtcgccg gcgtcgatct cctccatcag
     1201 cggcagcccc gtgctcagca cccgacgcag ccgcgcgtcg agtccgggca ggggctcgat
     1261 cggctgcacc agcgccagcg gccggccgat gtcgttctgg tcgagcgcgt acaggcgcgt
     1321 cgcggacggg ctgaagcggc agacgcgcag gtcggcatcg agcacgatct gcatctggcc
     1381 ggccgagccg agaatctcgt tcagctcggc gttgagcacc gccagttcgt tgagccggat
     1441 ttccagctcg gtgttggtgc tttccagctc ctcgttggcc gcctgcattt cctcggaggt
     1501 ggcttcgagc tcctcgttgg cggcctgcat ttcctcgttg gtggcctgca gctcgtcgtt
     1561 gacggtggcg gcggcggcga gcgcttcctg atagcggcgg ttcaggtcat cgagctcgct
     1621 ttcgaagccg atgttgccct cgatgccgga gttgtgcgcc gggtccttcg ccagcaccga
     1681 ctgggcctcg aagctcacca gccagccatg gccgagctcc tccccgtgca gcggccggat
     1741 gctgagctgc cagcggccgg tggccgagcc gtagccggtg atctgcggca gatcgacgct
     1801 gtcgatgccg tccttgacgg tggcgcgccg ggcccggctc agcgccacct gataggccgg
     1861 tcgccacggt tccggcagca ggttcgcgag gcgcaggtcg ggttcgttgc gcacgaagcc
     1921 gagaaagctc ctgacctcgc cgaagaagtg caggcagttg ccggtctcgt cgatcaccac
     1981 cgcattcggg gccagcgccg tgatcacggc cccgtacagc ccgagccggt ttctggccgc
     2041 tgccggcagc gtcggcagcg tcggcgtaca gcgtgctccg gtcggggggt gggcccgggc
     2101 gggccggcgc acgaacagcc gggcactgcc atcgagcggt gtgaacagcg ggctttcggt
     2161 gcggatcgat tcgcgccgcc cgagcagcag caggccttcg ctgccgaggg cggcatggaa
     2221 gacggcgagc agccgttcct gggcggcggg gcgcaggtag atgaacaggt tgcggcaggt
     2281 gacgagatcg aggttcagga acggcggggc cgagagcacg tcatggcgtg cgaacaccat
     2341 gcgcgcccgg atcgccggag cgatcacgta gccgccgggc acctcggtga agtgccgggc
     2401 gaggatatcg gcgggtacgc gccggcgcac cgcttcggtg gcgtagcgcc cgcggcgcgc
     2461 gatcgcgatg gctcgcgcat cgatgtcggt cgcgaagatc ttccagtcga gcggctggcc
     2521 gctgcccgtg cgcagcccgg agagcatcag ggccagcgaa taggcttcct cgccggtgca
     2581 gcaggccggc acccagaccc gcagcgtgtg tccgtccggc aggcgccggg tggtcgtttc
     2641 gagggcgctg gccagcgccg agaaggcgcg ccggtcgcgg aagaagtgcg tgacgccgat
     2701 cgagaaggat tcggccagcc gccgccgctc gatcgggtcg gcggcgaggc gtgcttcata
     2761 ggcgctgagc gccgcctgcg tccgcccgcc cgcgcagcgc agcacctgcc ggcgcagcgt
     2821 gccggacttg tagtcggtga agtcgtgccc gtcttcggtc aggcgctcaa ggatgcgctc
     2881 cagggtggcc gtcgcggcct gatcgaacgg aaacgaatcg gacatcggtg caactccatg
     2941 ctgcaacggc aagggcggtg aaacgggaat atgaccagaa gacagacagg cacgcgagac
     3001 ggcggatgtt ccgtcctgtc cggtgcactc cggcgagcgc tgccatgatc ccgcgcccgc
     3061 cgggtttcag tcacccttgt catcggccag cgtccggatc agggcttcga cgaaggcgcg
     3121 cgtcttcgcc ggcatcaggc gccggcccgg catcacggcc caggccgtca ccggtggcag
     3181 tgtccaggcc ggcagcaccg gcagcagggt gccggcggcg agatgggggc gcacgaagcg
     3241 ttcgtcgatc gcgaccagtc cggcaccggc gagcgccatg cgcaccagca gttccaccga
     3301 attggcgccg aagcgcgagg cccaggggcc gtgccattcc tgcaggccgt tctgcaggct
     3361 catcggcagc ggccggccgg cctgcggggc gagcagcagc gcatcgtgac cggcgagctg
     3421 ctccggggcc tgcggcgtgc cgcgccgggc gaggtagtcg ggactcgcgt acaggccgcg
     3481 cgcgaagctg ccgagccggc gcgcgacgag gctggcgtcc tcggccagcg gtcccatgcg
     3541 cacggcgaga tcgaagccgc cggcgatcag atcgacctgg tgcggcgaca gatcgagctc
     3601 gacgctgacc gccggatacc cggcgccgaa gcgcgcgagc atcggcacca gcatcagcac
     3661 ggccagatcc ggcggcatcg tcacgcgcag ccggccctgc ggcacggcct tgcgccgttc
     3721 ggcgagctgc acggcggcct ccatctcctc ggcgagccgg tgcgcgtgtt cgagcagcgc
     3781 ctcgccgaac tccgtcagca ccagccggcg cgtggtgcgc gtcagcagcc gctcgccaag
     3841 cgtggtctcg aaggcggcga gccggcgcga cacggtcgac ttcggcagac cgagtgcggc
     3901 cgccgcccgg ctgaagctgc cggcatcggc aatgcgcgca tacagcagca ggtcatcggc
     3961 agcgaggttc atcggcggtc tccggcatgc ggtaattgtc ctgaatatgg aacattcata
     4021 tccggattac cggcttctgg agcatggatg gaaccaatac agtctgcttc acccgcagac
     4081 cgccctgcgc cgaaccccca gccgaccgga gatgccgcga tgagccagaa ccagcacccg
     4141 aatgcgaacc cgaccgcgaa cccgaccgtc acccgcatcc tgcagatcaa tgccagcatc
     4201 cagctcgatg cctcgcagtc gacgcggctc gccgacgcca tcgtcgcccg tctgcagcag
     4261 gcgcatccgc aggccgtcgt gcagcggcgt gatctcggcc gcgcaccggt gccggccctc
     4321 gatgccgcgg cgctggccgc gctgttcacg cccgaagagg cgcgtacgcc ggagcaggcc
     4381 gcacgcgtcg cggccgacga tgcgctgatc gccgagctgc aggccgccga cgtgatcgtg
     4441 ctcggcgtgc cgatgtacaa cttcggcatg ccggcgcagc tgaaggcctg gctcgatgcc
     4501 gtgacccgcg cccgcgtcac cttccgctac accgagcagg gaccggtcgg gctgctgacc
     4561 ggcaagcgtg tctatgtcgc ccacgcccgc ggcggtcgct accgcggcac ggcgctcgat
     4621 cacgtgacgc cgcagctcga cacgcacctc ggcttcatcg gcctcggcga tgtacgccac
     4681 gtctacgccg aaggcttcgc gatgggcgac gaagcacgcg cggccggtct tgccgatgcg
     4741 gcgcgacagc ttgcggcgct gtttgacgaa gctgcggatg ccgtcgccgc ctgaagccgg
     4801 cacccggcgg gccctggagc ggcctgcatg cgggcagcac gatcgtccgg cgtgctgccc
     4861 ggtgcgcgca ccccggtgct gcgcaatccc cgacccccag aacccccgcc ggaggcctcg
     4921 ccatgaacct gcctgctgcc gattcccccg tcatccgtca tccgcgccgc gtcgagggca
     4981 tcgtcgccgg tcaggcgact tcggacggcg ccggtgtgcg cctcgtgcgc ctgctcgggg
     5041 ccgagctgca gcggcggctc gatccctggc tgatggtcga tgccttttcc agcgaccgcc
     5101 cggacgatta cctggccggc ttcccgtcgc atccgcaccg cggcttcgag accgtcaccg
     5161 tcatgcacgc cgggcgcatg cgccaccgcg acagtgccgg tcacgagggc ctgctgcgcg
     5221 gcggcgacgt gcagtggatg atcgccgggc gcggcctcgt gcattcggag atgccggagc
     5281 aggaggacgg gctgatggcc ggctaccagc tctggctgaa cctgccggcg gccgacaagc
     5341 tgcgcgcccc ggcctggcgc gacatcccgg cggcggcgat tccgcaggtg cgcaccgccg
     5401 ccggcgtgca ggtgcgcgtg atcgccggtg cgagccacgg ggtcgagggc gccgtgcagc
     5461 ggccgcacac cgcaccgctg ctgctcgacc tgagcctgcc ggccggcagc cgcttcgcgc
     5521 agccgctgcc ggcggcgcac cacggcttcg tcatcgtgcg ccgcggtcag ctggcgatcg
     5581 gctcccgcac ggtcacggcc ggccgtctgg cgctgctcgc gcaggaggcc gacagcgacg
     5641 gcgtcgtgct gaacgcgggg caggaggatg tcgaggcgct gctggtcgcc ggccgcccgc
     5701 tcggggagcc gatcgtgcag cacgggccgt tcgtgatgaa cacgcgcgcc gagatcctcg
     5761 cggccttcga ggactacgcg cgcggctcgt tcgacacggt ttcgccgcag cctttcgact
     5821 gagcctgccg gcgcccggcg gagggcgggt gcgtcgtgcc gcgggcgagc agggcgtgca
     5881 gttccgccgg tgcccgcggt ggcgcgaaca gatagccctg cacctcgtcg cagtcctggc
     5941 cgagcaggaa ttcgagctgg gcctcgtcct cgacgccctc ggcgatgacg ctgaggccga
     6001 gcgtgtgggc gagggcgatg atcgcctgca cgatcgccgc cgaatccgag ttgtcgggca
     6061 ggtcgcgcac gaaggcccgg tcgatcttca gccggtcgag cgggaaggtc ttcaggtagg
     6121 agagcgacga ataaccggtg ccgaagtcat cgatcgccac gccgacgccg gtgcggcgca
     6181 gcgccatcag caccccgacg gtgcgcgccg ggttgtccat cagcagcgat tcggtgatct
     6241 cgatctccag ctgcgccggc ggcaggccgc tcgagtgcag cgcatgcgtg atccccgtga
     6301 tcagcccggg atcggtgaac tggcgcgccg acaggttgac ggccacgcgc ggcggccggg
     6361 ggcagtgccg ctgccagccg gcggcctcgt ggcaggccgt gtcgagcgcc caggctccga
     6421 gcgtgcggat caggccggtc tcctcggcga tcgggatgaa ctcggccggt gacacggcgc
     6481 cgaggcgcgg atgattccag cgcatcaggg cctcgacgcc gatcacgcgc tcatcggcca
     6541 gcgcgatctg cggctggtag accagggaca gctcgccgcc gctgatcgcc gtgcgcagcg
     6601 agcgttcgag cagcgcgcga cgctgcatgc gctggccgag ttgcggatcg aagccgaacc
     6661 agccctcgcc ggaaacgcgc gcctggcggg cggccgcgac cgcgtggcgc agcagcgtct
     6721 cgccgtcgcg accatcgcgc ggggccagcg catagccgat gtcgacgctg acgctgaccg
     6781 gctcgggcag caccggcagc ggttcggcga ggcgcgtgtg caggccggcg acgaagcgcc
     6841 cggcggcctg ctcgtcgggc agcccggtcg ctgccagcgc gaactcgtcg ccggcgatgc
     6901 gcgcgagcag gcaggccggg cgggcggcga gggcgttcag gcgctcggcc agcgcctgca
     6961 gcacgcggtc cccgccgctg cgcccgagcg tctcgttcag gcgctggaag cggcgcaggt
     7021 cgaggttcag caccgcgcag caatgttcgg gcgccatgcg ctgcagcagc cccggcagcg
     7081 cggcgatgaa gctcgcgcga ttcggcaggc cggtcagtgc gtcatggctc gccagccagt
     7141 cgatgcgcgc gcgcatcgcc gtgtgctcgg acaggtcggc gaacaccgcg acgtggtgca
     7201 tgatcacgcc ggcggcatcg cgcacgacgc tgatgctcag ccattccaga tattcgcagc
     7261 cgtccttgcg ccggttgcag atctcgccgc tccagctgcc ggtgtcctgc agcgagcgcc
     7321 acatcgccgt gtagaactcg cggccgtggc ggccggaggc gagcagcgcc ggcgtctgtc
     7381 cgatgacctc ggacggctgg tagccggtga tggtcgtgaa cgccggattg acgcgcagga
     7441 tgcggttgtc ggcatcggtg atcaccgccg cctcgcggat gttctcgaac acgctggcga
     7501 ccaggcgcag gtcggtttcg gcggcgttgc gccggctgcg atcctccatg cgcacgaggg
     7561 cgtggctgat gtcctgcgcg atgcgcgtga tcaggcggcg cgatgccgca tcgggcggcg
     7621 gagtcccgag ctggcggaac cacagcacgg cgaccggctg gccgtccggc gcggacaccg
     7681 gcagcagcgc atcgacgctg ccgtcgcggt tttcgtccca gcgcgccgcg ccgccgctca
     7741 gcacctgctg cgcgcgttcg cgcgcgagcg gatcggccag atcagcggcc cgggtcgcaa
     7801 gcgcggtctg cacgcccggc accggctcgg ccagcgtgcg ggcgaggctg tcgtgcagcc
     7861 ggtccggatc ctcgcagtcg aacatggcct gatcgacgcg tccgatcagc gcctgcaggc
     7921 gatcgagccg ctgctggcgg cgtccggcgc tggcgcggaa acgctgcagc agcgcggcgg
     7981 tcgccgccag cgtcagcgtc agcaccccgc cgatcagcag cagctgcagt gcgaactgat
     8041 cgagcggggc ggcgatgtgc cggtattcga cggcactggc gagcacgagg ccgccggctt
     8101 cggccggcag cgccgcgaag ccgaccagct ggtcacgggc atgcgcctgc gcatcgcgcc
     8161 agacctgcag ccggaacagg ccgctgccgg cggtttcccg gcgcagccgt tcgcgcagca
     8221 cgccgaagtc atcggcggtg ggggaggagg ccgctgccgc cggaaccgct ccggccgggt
     8281 gcgcctgcca gagcacccga ccctcggcgt cgaacagcca gccctcgaca tcgtcgcgtt
     8341 tcgtcagcgc ccccagggcg cggctggcca gcgtcgtcag atcgagcgcg aggcgcagcc
     8401 gggcggtttc gccggctgcg gacgtcagcg gttcggtgag caccaggacg gtgcgcccgg
     8461 cgagctgcag caacagcgcc gcggcggtgt cgtcctgcgc cggcggcggc agggacaggc
     8521 cggcgtgggc gtccagccgt gcatcggcgg cacgccgttc gatggcgacg accgtgccgg
     8581 cgtggatgaa gcggtagtga tccagcaggg ccgcaagcat gtccggcggg gtgtcggtcg
     8641 catccagccg tgcggagcgc accgccagcc atttcagcga ctcgcgatac tcgcgcagca
     8701 gttcggcgaa ctggctggcg ccggcgacgg catcgatgcg catctgctgg ccggcccgat
     8761 cgagcagcgt gctgcgaaag tccgtgtaag cctgatacag catcaccgcc agcgtcagca
     8821 gcagcagggg caacagcatc cggatcagga acagcaggcg ggaggcttcg ctctcacgca
     8881 tcatcgccgc ccgcacgtgg cggagcggcg ccggcgacgg cgctcgcgac ggcggccggg
     8941 gcggggccag gcagtcgcac gcagatcagg gcagaaggag gggagtgcag cttgcgctcg
     9001 ctcatcgtgg tgtcctcttt ttgatcagcg cgatggagcc tgcaacggtc gcggataccc
     9061 gccggcgacc gcagcccgga ggctgcaatc ataatgcggc aggtctgcgc cgtagcggta
     9121 gatggacaag cgcaaggccg gtttcatggg tatcgaaacc atgttccgct gctggacaac
     9181 tggcggtaat gctggctctg gcagggattg cgctgtgcat ccgcgaccgc tcctgctatg
     9241 caatgtcaag caaacttgac aacaatcgag gtcaatggaa acaatgctta ccttttcgat
     9301 cccgtcggcg gtccgatgag tgcagccgat ctgatggcgg cggcgcagcg cttgtcggcg
     9361 gccctggcgc cgctgcattt ccgggcgccc gtgacccacg tctacaaccc gctcgactat
     9421 gccggtttga catatgggca atacctgcaa cgctggggag acggtcgccg gcgcgtgctg
     9481 ttcctcggca tgaatccggg acccttcggc atggtccaga ccggcgtgcc gttcggtgaa
     9541 atccacgccg tgcgcgactg gctgggcatc gacgccccga tcggtcgccc cgccgtcgag
     9601 catccgaagc ggccggtcga aggctgggcc tgccggcgtt cggaggtcag cggccggcgg
     9661 ctgtggggct tgttcgccgg gcgtttcggc acgccgcagg ccttcttcgc cggacatttc
     9721 gtgctgaact ggtgcccgct ggcctttctt gccgacggcc gcaaccttac gcccgacagg
     9781 ctgccgtccg atgaattcgc gccgctggca gcggcctgcg accggcacct gcgggaagcc
     9841 tgtgccgcgc tcgaacccga atggctgatc ggcgtcggcc gctttgcacg cctgcgcgcg
     9901 cagcaggcgc tcaccgggcc tgctgcgccg cgcatcggcg agatcctgca tccgagcccg
     9961 gccagcccgg cggccaatcg cggctgggcg gcggcggcga gtgcacagct gcaggaactc
    10021 ggggtctggg gcgcctgatc aggcgttgtc agcgtttcac gatgcgcagt gccatggcag
    10081 gactatgctg cgctgaccgt gcatgccggt gaatgtccca gccgatgatg agcgccacct
    10141 ccgagctgcc gagcaatgtc gtcgcagaag cccgcctgcc ggcggtgctg ctggcgatcg
    10201 tgctgggctg ggcagcgctg atcggggcgc tcgcgtattt caccgagcgc gacatcgccg
    10261 gagaccgggc gcagaccctg gaaaccgctg cgcagcgcgc cgaaaccctg ctgccgctgt
    10321 tcggcaacag cgtgcagcgc gcgctcgacg atacccggct gctgctgtcg gcgctggaac
    10381 tggcgctgga gtccaacggc ggcgatggcg cggcccgcga cctgctgcag cgcttcggcg
    10441 caccgcagga cggcatgcgc cagcgcatca gcatcgccga tgcccgtggc cgcctgatcg
    10501 cctcgaccgc cgacctgccg gccgccagcc tttcagtggc cgatcgcgat tacttccagc
    10561 ggctggcggc cccggatgcg ccgggctgga tcctcggcaa gccggtcatc agtcatgtca
    10621 ccgacgactg gaccattccg cttgccgtgc gtcgcgttgc cgccgatggc cgttttaccg
    10681 gcgcgctgat caccgccatc gatccgcagt ggctgctgac gatggccggc tcgatggacc
    10741 tcggccgcac cggcacggtc ggcgtcgtcg atttcgatgg cacggtctac ctgcgccgcg
    10801 acatcgacct gcaccatgcc ggggccgcca gcacgatcgg caccaccaat ccgaggctga
    10861 tcgtgctcga accggcgcgc accgcaccgt ccgggcgctt cagcggcacc tcggcgctcg
    10921 acggcctgcg ccggctcggc tcctaccgcg tgctgccgca gtacgggctg atcgtcgcct
    10981 acggtctcgg cgaggacgag gtgctgaccc agcacatgcg ccgcgccggt gccgagcgcc
    11041 ggctggcgct gctctccggc gtgctgctga ccgcgatcgc cggcgggctg ttcctgtggc
    11101 ggctgcggga ctggcgccag gcgcggcagc tgctggcggc ctggcgccag gcgcacaccg
    11161 ccgcgcgcac cgacgtgctg accggcctcg gcaaccgccg cggctacgag gaacacatcg
    11221 gggcgctgct cgcgagccgt ccgccgatgc cgtcgagcct gctgtacatc gactgcgacg
    11281 acttcaagcg catcaatgac agcgccggcc acgaggccgg cgatgccgtg ctgtgcgcac
    11341 tcgccgccag cttcagcgac agcgtgcgcg ccagcgatta cgtcgcgcgc ctcggcggcg
    11401 acgagttcgt cgtgctgctc gccaatgccg gcgaggtggc ggccgcggcg atcgccggca
    11461 agctcaagag ccgcgccgag accgccgtcg ccgccatcgg gctgccggtg acgctcagca
    11521 tcggccagat caccttccgc cgggtccacg acgccgcggc gctgacccgc gaggtcgatg
    11581 ccgcgatgta cagcgccaaa cgcgagggca agaaccgcat cgtgcgcgtg acttcgatcg
    11641 gcacctgagc cgcgccggcg cgatccggtc gtggttcagc ggttaagctg cgcgccgccc
    11701 ggccgctgcc ggtccgcgga agcccgtgca tgacgacgta cccgatgccg aaccgaaatc
    11761 tcccggcccg cccggcgccc tggctgctgc tggtgctctg cctgctgggc ggtctcggcg
    11821 cctgcggcga acgccgcgcc gaaccggccg cggcggccgc aacggcggca gccccgatcc
    11881 aggtcggacc cgagaacctg tggacggtcg tgcgcgcccg cgtgcagtcc ggaccgctgc
    11941 tcaccggcac gctgcgcccg gagcggcgcg ccgaggtgcg tgccgagatc ggcggcacgc
    12001 tgcgcgaagt cagcttcgag ccgggtcagg cggtggcggc cggggccgtg ctggcgcggc
    12061 tcgatgaacg cgccgtgcgc gatgcccgcg attcggcgct ctcggcggtc ggctcggccg
    12121 agcgcgcggc gctgctggcg cggcgcaacg ccgagcgcgg cgacgagctg ctgcgcaccg
    12181 gcgcctggac gcgccgccag caggaggatg cctgggcggc gtcgatcacc gccgatgcca
    12241 cgctcgccga tgcccgcgcg cggctggccg cagccgacaa gcagctcgcg tacaccgtcg
    12301 tgcatgcgcc gatggccggt cgcgtcgccg agcggccggt cgcggccggc gatgtggtgc
    12361 cggccggtgc gacgctgtac gtgctggtcg atccggccag catgcgcctc gaagccgcgg
    12421 tgccggccgc cgagctgccg cgcgtgcagg ccggcgcggc cgtggagttc acggtgcgcg
    12481 gctatccgga ccggcgcttt cgcggcacga tcgagcgcat caatccgatg gccgacccgg
    12541 tcacccggca ggtgacgctg tacgtggcgc tgccgaacgc cgacggcgcg ctgctcggcg
    12601 gcctgtacgc cgaaggccgc gtcgccgccg acgtgcgcga ggcgccggtg ctgccgatcg
    12661 atgcggtgcc ggccggggcc gcgcaggcga gtctctggcg cgtgcgcgag ggccggctcg
    12721 aaagcgtcac ggtcgaaacc gggctgcgcg acgccgaggc cggatacgtc gagatccgct
    12781 ccgggctcga ggtcggcgac cgcatcctgc tgcgcccggc gcgcacgctg caggccggca
    12841 cgccggtcga gatcctgccg gtgctgcgct gatggagggc agggacgggg cagggcaggg
    12901 gcgcggcatg cgcatctcgg atttcagcat cgagcagccg atggtgacgg tggtcgcgat
    12961 gctggcgctg gtgctgttcg gcttgtttgc gctgctcgcg ctgcacaccg acgaattccc
    13021 ggacatcgag cagccggtca tcgtcgtcgg catcccgtac cccggcgcct cacccgaggg
    13081 cgtcgagcgc gagatcctgg agcgcgtcga ggaatcgatc accggcatcg ccggcatcca
    13141 cgagctgcgt tcgagcgcga gcgacggcta tgcgcagctg atcgtgttct tcgagttcgg
    13201 caagaacatg ctcgaagccg ggcaggagat ccgcgaccgc atctcggcgg tgcgcgaccg
    13261 cctgcccgac gagatgaagg agccggtgct gacccgggtc gatccggccg acatcccggt
    13321 ggtgtcgctg acgctggcct cggaccggct gacgccggtc gagctgaccc ggctcgccga
    13381 tcccggcatc gtgcgcgagc tgcgtcaggt gcccggcgtc gcgcaggtcg atgttgccgg
    13441 cggccagacg cgcgagctga acgtgcagct cgatccggcc gcgctcgccg aggccggggt
    13501 cacgctcgcc gagctcgtgc aggcgctgaa cgcgcagaac ctcgccgcgc cggtcgggcg
    13561 cctgacgcac ggggccggcg agcagacgct gcgcctgcgc ggccggctcg acggcccgga
    13621 ggccttcgcg cagctcgtgg tcgcgcgccg cggcgagcgc gtgatccggc tcgggcagct
    13681 cgcgcacgtc agcgacggcg ccgaggaggc gcgttcgctc gccttctacg acggccgccc
    13741 cgcgatcggc atcggcgtgc tgaagtcgcg cggcgccagc acgacggcgg tcagcgccgc
    13801 gctgcgcgcg caggtcgcgc aggtgcaggc gacgctgccg gccggcgtgg atctgcgcat
    13861 cgtgcaggac gccggcgagc gcgtcggcca ttcggtcgcc aacgtcggcg agcacctcgt
    13921 gctcggcgcg ctgctgacgg tgctgactgt gttcctgttc ctgaactcgt ggcgctcgac
    13981 cgtcatcacc ggcctggcgc tgccggtgtc ggtgctcgcg gccttcgtcg cggtctgggc
    14041 ctgcggcttc acgctgaacc agatgtcgct gctcgggctg tcgcttgcga tcggcatcct
    14101 gatcgatgac gccatcgtcg tgcgcgagaa catcgtgcgc catgtgcacc tgggccgttc
    14161 gcacctcgat gccgcgcgcg ccggcaccgc cgagatcggg ctggcggtga ccgcgaccac
    14221 gttttccatc gtcgcggtgt tcgtgccggt ggcgttcatg tccggcgtcg ccggccagtg
    14281 gttcaagccc ttcgcgctga ccatcgcctg cgcggtgctg gtgtcgctgt tcgtgtcgtt
    14341 ctcgctcgat ccgatgctgt cggcctactg gcccgatccg cagcccgagg cgcagcggcg
    14401 cggctggctg acgcgcgcgc tcgatcgctt caacgccggt ttcgaccggc tgaccggacg
    14461 ctacgtgcgc gtgatcggct gggcgctcga tcaccggctg gtgatgacgg cgctcgcgct
    14521 cggcagcttc ttcggcgcgc tgtggctgca ggcgcagttc ggcggtgcgg ccttcgtgcc
    14581 gccgagcgac cgcagcgagc tgcgcatcgt catcgacacg ccgcccggct ccagcctcga
    14641 acacacgcgc gtgctggccg agcgcgtcgc cgggcttgcc cgcgcgctgc ccgaggttcg
    14701 ctacacctat acgcgcgccg gcagcgccgg cgatttcacg gtcggcggcg tcgataccgc
    14761 gaccgtctac gtgcgcctga aacccaagtc cggacgctcg cgcacgcagg agcagatcgg
    14821 caacgcgttg cgcgcgcagg cgcagcagct cgccggcgcc gaggtcgcgg tgttctcggg
    14881 cggcttcggc ggcgcctaca agccgatcca gctcagactg atcggcccgg atgcgcgcga
    14941 actcgccgag accgcccagc gcctgctcgc ggcggcgcgc gaggtgccgg gcatcgtcga
    15001 tgcgaacctc tcgacgcgcg ggcgcaagcc cgagtacgag ctgacggtcg atcgcgggct
    15061 cgccggtgcg ctcggcatca cggtcgccga catcggccag ggtctgcgcg tcgcctatgc
    15121 cggactcgaa gccggcgact ggatcgaccc ggacggcgag acccgcgacg tgaccgtgcg
    15181 cctcgctccc gagtaccgcg aactcgccgc cgggctggcg cgcctgccgg tcgctgcaca
    15241 cgccgccgac gggcgcaccg tgtcgctgcc gctcgggcag atcgtcaccg tcacgcccgg
    15301 gctcgggccg cggcgcatcg atcacttcga caccgagcgc gtgatcgtca tcgaggccgc
    15361 gacccagggc gtgccgctcg ccgaggccgt gcgccggctc gacgcgcggc tggcgcgtga
    15421 gccgctgccg ccgggcctgc gactgcagca cggcggcgag gccaaggatc aggccgaggt
    15481 gttcggcaac atcttctcgg cgctcggcat cgcgctgctg ctgatgtacc tgatcctggt
    15541 gctgcagttc ggcagcttcc tcgatccggt gccgatcatg gcctcgctgc cgctgtcgct
    15601 gatcggcgtg gtgctggcgc tgctcgtgac cggcgacacg ctgaacatca tgagcctgat
    15661 cggcgtgatg ctgctgatgg gcgtggtcgc gaagaacgcg atcctgctga tcgacttcgc
    15721 caggcagggc cgcgcgcgcg ggctggcgct gcgcgaggcg ctgatcgagg ccgggcgcgt
    15781 gcggctgcgc ccgatcctga tgacgacctt cgcgctgacc gccggcatga tcccggtcgc
    15841 gctcggccac ggcgaagggg cggacttccg cgcgccgctc gggcgcgccg tgatcggcgg
    15901 cacgctgacc gcgacgctgc tgacgctgct ggtgatcccg accttctacg agctgaccga
    15961 cgaatggcgg acgcgggtgc agggctggtg ccggcgacgc tgagcccgaa gcggcctgca
    16021 cgcgctcgga tgagctgatg cgaagccgta ggggctctgg ccggcgatgc gcgcattgca
    16081 tggctgcgtt tcagaaatcg gaggacttgc gggcatccgg tgccgcaatg gagcaaccgg
    16141 cgccggatcg gcaggtcatg gccagccggc cgcgattgcg catccgccgg atcgtgcgcc
    16201 ggaagcctgc gacgaaattc gacggatcag gcgcttgacg gccgtttccg gccgaaatcc
    16261 gggctttccg ggcaaattcc gggctgcgcc gcgctcccgg cagtttcctg ctgcagggaa
    16321 ttcttgcggt gcagcagcgt ctgagctcgg cgtcgtgcct gggatcgctt cgcagagcgg
    16381 tcccgcaagc gcatgatccg gcctgccgtg atggccgcat aagatgc
//
LOCUS       DAOQIE010000038        16363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1660_length_16363_cov_8.135639, whole genome shotgun sequence.
ACCESSION   DAOQIE010000038 DAOQIE010000000
VERSION     DAOQIE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1660_length_16363_cov_8.135639"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PK144_04015"
     CDS             <1..1163
                     /locus_tag="PK144_04015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF64924.1"
                     /translation="VIQRADPHIGLLHRGTEKLAESKPFNQSIGYMDRLDYVSMMCNE
                     HGYVLAIEKLLGVEVPLRAQYIRVMFDEITRILNHLMWLGAHGLDIGAMTVFLYCFRE
                     REDLMDCYEGVSGARMHATYYRPGGVARDLPDSMPKYEYNAYRSKAEIDALNETRGGS
                     MLDFLEAFTERFPGRVDEYETLLTENRIWKQRTVGIGVVTPERALQLGFSGPMLRGSG
                     IEWDLRRKRPYEVYDRLDFDIPVGTHGDCYDRYLVRVEEMRQSNRIIRQCIRWLRDNP
                     GPVMPEGHKVSPPKRTEMKESMEALIHHFKLFSEGYCVPAGEVYAAVEHPKGEFGVYL
                     ISDGANKPYRLKVRAPGFAHLSAIDEMVRGHMLADVVAVIGTQDIVFGEIDR"
     gene            1163..1660
                     /gene="nuoE"
                     /locus_tag="PK144_04020"
     CDS             1163..1660
                     /gene="nuoE"
                     /locus_tag="PK144_04020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNF64925.1"
                     /translation="MSQNKHAVLSEHACHEIDRWVAKYPPERKQSAVLAALREVQHEN
                     GGYLTNELMDAVADYLGLPAIAVYEVASFYSMYELKPVGRHNIAVCTNISCMLRGSDE
                     IVAHIEQKLGIRLGESTADGKFYLKKEEECLAACASAPMMQVDHVYYENLTTAEVDRI
                     LDSLE"
     gene            1668..2966
                     /gene="nuoF"
                     /locus_tag="PK144_04025"
     CDS             1668..2966
                     /gene="nuoF"
                     /locus_tag="PK144_04025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNF64926.1"
                     /translation="MFLNGMLYATKTLENPWSFDTYVQQGGYEAWKKILRGELTSDEV
                     IDIVKASGLRGRGGAGFPTGLKWSFMPRKAPGQKYVVCNSDESEPGTCKDREILIYNP
                     HALVEGMAIAGFAIGATAGYNYMRGEFMDEPYLHFEQAVNEAYAAGLLGRNVQGSGID
                     FDLYPSLGAGAYICGEETALLESLEGKKGQPRFKPPFPANFGLYGRPTTINNTESLAS
                     VPYILRNGAQWFADLGIPGAGGCKIFSVSGHVAKPGNFEIRLGTPFKDLLELAGGVWK
                     GRKLKAVIPGGSSVPVVPAETMLKVNMDYDSISKGAGSMLGSGAVIVMDETTDMVRAL
                     QRLSRFYFSESCGQCTPCREGTGWLYRMLTRIVEGQGRPEDIDLLDSVASKIEGRTIC
                     ALGDAAAMPVRSFVKHYRHEFAYYIEHKRSMVPGANAVAA"
     gene            3003..5342
                     /gene="nuoG"
                     /locus_tag="PK144_04030"
     CDS             3003..5342
                     /gene="nuoG"
                     /locus_tag="PK144_04030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNF64927.1"
                     /translation="MSEDLVNIEVNGTPLQARKGQMLIEVTDAAGITVPRFCYHKKLS
                     IAANCRMCLVEVEKAPKPLPACATPVMDGMKVQTRSPKALAAQKGTMEFLLINHPLDC
                     PICDQGGECELQDVAMGYGGDVSRYVERKRVVKDKDIGPLVATEMTRCIHCTRCVRFG
                     EEIAGLREMGATGRGENTEIGTYVRKSLASELSGNIIDLCPVGALTAKPSRYTARSWE
                     MSQHAGIAGHDGIGSSVWHHVRYGKIVRTVPRENEAVNEVWLSDRDRFSYEGLYSSDR
                     ATQPMVRAADGALAITGWEAALEATAAGLKAVIAKHGAAQVGVLVSPSATLEELFLAQ
                     KLARGLGIASIDHRLRQIDFTAAAGGYPSLGLALADVPRLDAALVIGSNVRMELPLFA
                     HRLRQASLKGGKVAFLNPVRYAQTLDPAAEFVVSPAAMVGTLARLAAAVAGVKGVNVP
                     AALAAAGEPSEAERGYAETLAGAGRAAIFLGQLALSHPQYAQLQAIAGFLARSCGATV
                     GYLAPGANAAGASLAGALPAEGGQDAQAMLAAGLKGYVLIGIEPEFDCAAPAVAARAL
                     AGAEFVVALGTHLGGTVEREADVVLPVAACVETSGTFVNLEGRWQDFQAVARAPGQVR
                     PGWKVLRVLGNLLGVAGFDHLSSADVAAEARSAADGIAATGAVEVAPADVQPAGEGLA
                     CIVEVPIYAIDPIVRRAAALQASPLAVSAEVRIGTAGAGQFGLTADARASVGRDGNAV
                     VLPVKIDDRIPDGCVWVPAGVEATGALGAGAGPVEVRPA"
     gene            5451..6473
                     /gene="nuoH"
                     /locus_tag="PK144_04035"
     CDS             5451..6473
                     /gene="nuoH"
                     /locus_tag="PK144_04035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNF64928.1"
                     /translation="MDSLLTLVLILLKIVAIVAPLMLSVAYLTYAERKIIGYIQVRIG
                     PNRVGPKGWLQPIADAVKLMMKEIVIPTGANRFLFVLAPVLSIGPALAAWAAIPLGDG
                     IVLSHIDAGLLYLLAMTSLGVYGVIIAGWASNSKYSFLGAMRSAAQIVSYEIAMGFAL
                     VGVLMAGGSLNLNEIVRAQSGSVLHWFWLPLLPLFIVYFISGVAETNRAPFDVAEGES
                     EIVAGFHVEYSGMTFAVFFLAEYANMILISALAATMFLGGWLSPFQGIPLLGALFTWV
                     PGAVWLILKTAVFLFLFLWLRATFPRYRYDQIMRLGWKVFIPVTIVWLAVVGVAKWAH
                     LAPWFD"
     gene            6495..6983
                     /gene="nuoI"
                     /locus_tag="PK144_04040"
     CDS             6495..6983
                     /gene="nuoI"
                     /locus_tag="PK144_04040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNF64929.1"
                     /translation="MAALKHYFRSFLLWELFIGLRLTGKYLFARKITVQYPEEKTPMS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIDMQQREDGTRRTTRYDIDLFKC
                     IFCGFCEESCPVDSIVETRFFEYAFEKREESIMTKPKLLAHGDRMEASIAADRAADAR
                     YR"
     gene            7005..7607
                     /locus_tag="PK144_04045"
     CDS             7005..7607
                     /locus_tag="PK144_04045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNF64930.1"
                     /translation="MGFEKFLFYVFAIVLVFASVRVITVRNPVHAALFLVLAFFTSAA
                     LWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDINVAPLREGFIRNAPLGIAVATII
                     VIEMALVLTSDRSGLVSGALQNYAADHSNTKELGSVMYTLYAYPFEIAALILLVAIVA
                     AIGLTMRRRQGVRYVDPAEQVAVRREGRVRLVNLAAEKKS"
     gene            7699..8004
                     /gene="nuoK"
                     /locus_tag="PK144_04050"
     CDS             7699..8004
                     /gene="nuoK"
                     /locus_tag="PK144_04050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNF64931.1"
                     /translation="MIALTDYLVLGAILFCLSVAGIFLNRKNVIILLMSIELMLLAVN
                     FNFIAFSRFLGDSAGQVFVFFILTVAAAESAIGLAILVVLFRNRRTINVGDLDTMKG"
     gene            8009..10021
                     /gene="nuoL"
                     /locus_tag="PK144_04055"
     CDS             8009..10021
                     /gene="nuoL"
                     /locus_tag="PK144_04055"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF64932.1"
                     /translation="MQTVYLSLVLAPLFGAIIAGLFGRQIGRSAAHWVTIIGVAISFL
                     LSLAVFKHHVIDGAPAYNATVYTWMVIDGIRLEVGFLVDSLTVMMITVVTFVSLMVHI
                     YTIGYMHDDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGVVSYLLIGFWFK
                     RPTAIYANMKAFLVNRVGDFGFLLGIAAVLMYFNSLDYQDVFHAAPLFANAKMQIIPG
                     VEWSVMTVTCILLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVS
                     RMSPLFELSTTALSFVLVIGSITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGV
                     SAYSAAVFHLMTHAFFKALLFLGAGSVIIGMHHEQDMRKMGGLRKYMPITWFTSLLGS
                     LALIGFPGFSGFYSKDSIIEAVHASHIPGSGFAYFAVIVGVFVTAFYSFRMYFLVFHG
                     EERFHHHDDAHGHGHDAHHDDDHGHGHHGGTPHESPWVVTVPLILLAIPSVIIGGKTI
                     APMLFGDFFQGVIHVAHEHDTLHHLASEFHSALGMAFHGLLTLPFWLAMAGVGSAYYI
                     YMKRPDIAEKTRAKFAPVYRLLDRKYGFDDFNQAVFADGSVVAGKALWKYGDAGLIDG
                     VVVNGSAKTIGWLAGVVRHVQSGYLYHYAFAMIIGLLGLLTWIVVR"
     gene            10042..11556
                     /locus_tag="PK144_04060"
     CDS             10042..11556
                     /locus_tag="PK144_04060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF64933.1"
                     /translation="MLSQFPLLSLVIWAPILGGIGVLFVGDDSPAKARRLALVASIAT
                     FVLSIPLWTNFSLGTADMQFQELYPWIPGFKINYHLGVDGFSMPLILLTTFITVLVVI
                     AGWEVIQYRVAQYMAAFLIMEGLMIGVFAALDAILFYVFFEAMLIPMFLVIGIWGGPR
                     RVYATIKFFLYTFFGSVFMLVALMYMYFKSGSFEILAFHNLKLGMTEQVLIFLAFVGA
                     FAVKVPMFPVHTWLPDAHVEAPTGGSVILAAITLKIGGYGFLRFALPITPDASASLDW
                     LILGMSLIAVVYIGLVALIQQDMKKLIAYSSIAHMGFVTLGFFIVFSILRATESTSGA
                     AMGIGGGMVQMVSHGFISGAMFLCVGVLYDRVHSRQIKDYGGVANTMPVFAAFMVLFG
                     LANAGLPGTSGFVGEFMVILSSFKGSFWIAFLASFTLILGAAYTLWLIKRVVFGEVVH
                     QHVAELQDVNAREFTILAVLAVAVLALGLWPQPLTDVMQASIERLVQHVLVTKL"
     gene            11634..13070
                     /gene="nuoN"
                     /locus_tag="PK144_04065"
     CDS             11634..13070
                     /gene="nuoN"
                     /locus_tag="PK144_04065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNF64934.1"
                     /translation="MTFVPPDLMPALPEIFVATMACLVLVIDVYLPEARRTTTLWLSI
                     ATLLFAALLTLATAPEARVPGFHGHFVRDAMGDVLKIGVYLSMAAALLYSRDYLRNRT
                     LFKGEYYVLALFAVLGMMVMISAASFLTVYLGLELLSLSLYALVAFDREDGRCSESAM
                     KYFVLGALASGMLLYGISMMYGATGSLNLASVAQAIAGKGDKTLVLIFGTAFLVVGLA
                     FKLGAVPFHMWLPDVYQGAPTSVTMIVASAPKLAAFALTMRILVEGVAPLGADWQQML
                     MVLAALSIAAGNVIAIAQTNIKRLLAYSTISHVGFILLGFVAGTAEGYAASMFYALTY
                     VLMTVGSFGLLILCGRAGVEIENISDLKGLNDRSPWLAFMMLVLMMSTAGIPFLVGFY
                     AKFAVLQAVVAQGFVGLAIFAVILSVIGAFYYLRVVKVMYFDKAEDDTTLSVSADAQV
                     LMGANGLAVMLLGVYPSGLMALCSAAIH"
     gene            13091..13420
                     /locus_tag="PK144_04070"
     CDS             13091..13420
                     /locus_tag="PK144_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HNF64935.1"
                     /translation="MGQDALVTLWLLLAAVAVNLPWLSDRLLLVRSAGAAGKPTWLRW
                     LEWLLLAVLVTAAGLASEWKATGEHHPQDWEFYVVAICLSLVAAMPGFVYRYQWLPLA
                     SRRTRGL"
     gene            13668..14123
                     /gene="rimP"
                     /locus_tag="PK144_04075"
     CDS             13668..14123
                     /gene="rimP"
                     /locus_tag="PK144_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNF64936.1"
                     /translation="MRADPYKLRELLGPSIAGLGCELVGIEYHPNSANALLRIYIDRA
                     EGVTVDDCERVSRQVSALLDVADPVPGHYTLEVSSPGLDRPLFEPEHFQRFTGRQARV
                     QLGMALDGRRRFVGEILGLRDGELLLRCEGTEVAIPLDRIEKARLVPEF"
     gene            14149..15645
                     /gene="nusA"
                     /locus_tag="PK144_04080"
     CDS             14149..15645
                     /gene="nusA"
                     /locus_tag="PK144_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNF64937.1"
                     /translation="MSKEILLVVDAVSNEKGVAKTVIFEAIEAALASAAKKRYAGDPD
                     VRVEINRKTGEYHTFRCWEVLADDIEPEFPDRQLPLGRAVELDPAAETGALITEPLEN
                     AAFGRIAAQTAKQVIVQKVREAERAQVVDQFLPRRGQLISGVVKRVERGNVLVDLGGN
                     IEAMVAREDMIAREPVRNGDRIRGWLKDVRHEPRGPQIFLSRTAPEFLIELFRLEVPE
                     VGQGVIEIKGAARDPGVRAKIAVKTNDQRIDPVGACVGMRGSRVQSVSTELAGERIDI
                     VLWNDNPAQFVINAMSPAEVISIVVDEDSHAMDIAVAEEKLSQAIGRGGQNVRLASDL
                     TGWELNVMTLAQAEAKSGQEAQAVLQMFMDMLSVDADVAEILVREGFSTLEEVAYVPV
                     HEMLEVEEFDQEIVDELRARARDALLTRAIAAEEQVGDAEPAEDLRRMEGMDEDLAFL
                     LAGKGVRTQEDLAELSVDDLIDMADIDEARAAQLIMTARAPWFSGDQG"
     gene            15666..>16363
                     /locus_tag="PK144_04085"
     CDS             15666..>16363
                     /locus_tag="PK144_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013716992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HNF64938.1"
                     /translation="MTEVTVKQFAAVVGTPVDRLLEQFAEAGIQVGGADSSITDQQKM
                     ELLAHLRKSHGRKESLAVAEPKKITLQRKTHSEIKVAAAPGQTKTVSVEVRKKRTYVK
                     REPLPGETVPVTETAPAEEFADDAAAIAETFAAEAPASTEAYTEAVPEPVAETGSSTA
                     RATPARPTVQRAAPVERRPVAPSTSVLARPRADQNRARANESLELPRVDNTRPKVVGV
                     VSKKQVARDDAAAAA"
BASE COUNT         2573 a         5321 c         5465 g         3004 t
ORIGIN      
        1 aggtcatcca gcgcgccgat ccgcacatcg gcctgctgca ccgcggcacc gagaaactgg
       61 ccgaatccaa gccgttcaat cagtcgatcg gctacatgga ccggctcgac tacgtgtcga
      121 tgatgtgcaa cgagcacggc tacgtgctgg ccatcgaaaa gctgctcggg gtcgaagtac
      181 cgctgcgcgc ccagtacatc cgcgtgatgt tcgacgaaat cacgcgcatc ctgaaccatc
      241 tgatgtggct cggcgcccac ggtctcgaca tcggcgcgat gacggtcttc ctgtactgct
      301 tccgtgagcg tgaagacctc atggactgct acgagggcgt ctcgggtgcg cgcatgcatg
      361 cgacctatta ccggccgggc ggtgttgccc gcgatctgcc ggacagcatg ccgaagtacg
      421 aatacaacgc ctaccggtcg aaggccgaga tcgatgcgct gaacgagacg cgcggcggct
      481 cgatgctcga tttcctcgaa gccttcaccg agcgtttccc gggccgggtc gacgaatacg
      541 aaaccctgct gaccgaaaac cgcatctgga agcagcgcac ggtcggcatc ggtgtcgtga
      601 cgccggagcg cgcgctgcag ctcggtttca gcggcccgat gctgcgcggt tccggcatcg
      661 aatgggatct gcgccgcaag cgcccgtatg aggtctacga tcgcctcgac ttcgacattc
      721 cggtcggcac gcacggcgat tgctacgacc gctatctggt gcgcgtcgag gagatgcgcc
      781 agtcgaaccg gatcatccgc cagtgcatcc gctggctgcg ggacaatccg gggccggtga
      841 tgccggaagg ccacaaggtt tcgccgccca agcgcaccga gatgaaggag tcgatggaag
      901 ccctcatcca tcacttcaag ctgttctccg agggctactg cgtgccggcc ggcgaagtct
      961 atgcggccgt cgagcatccg aagggtgagt tcggcgtgta tctgatttcc gacggtgcca
     1021 acaagccgta ccggctgaag gtgcgggcgc ccggtttcgc gcatctgtcg gcaatcgacg
     1081 aaatggtgcg cggtcacatg cttgccgacg tcgtcgccgt gatcggtacg caggacatcg
     1141 tgttcgggga gatcgaccgc tgatgagcca gaacaagcat gccgtgctgt ccgagcacgc
     1201 ctgtcacgaa atcgaccgct gggtcgccaa atatccgccg gagcgcaagc agtcggcggt
     1261 gctggccgcg ctgcgcgaag tgcagcacga aaacggcggg tatctcacga acgaactgat
     1321 ggacgcggtg gccgattatc tcggcctgcc cgcgatcgcg gtctatgagg tcgcgtcgtt
     1381 ctattcgatg tacgaactga agcccgtcgg ccggcacaac atcgcggtct gcacgaacat
     1441 ctcgtgcatg ctgcgtggct cggacgaaat cgtcgcgcac atcgagcaga aactcggcat
     1501 cagactcggc gaaagcactg cggatggcaa gttctacctg aagaaggaag aggaatgcct
     1561 ggccgcctgt gcgagtgcac cgatgatgca ggtcgatcac gtctactacg agaacctgac
     1621 gaccgccgag gtcgaccgga ttctcgacag cctggagtga gcgctcgatg ttcctgaacg
     1681 ggatgctgta tgcgacgaag accctggaaa atccatggtc gttcgatacc tatgtccagc
     1741 agggcggtta cgaggcgtgg aagaagatcc tgcgcggcga actgacttcg gatgaggtca
     1801 tcgacatcgt caaggcctcg ggcctgcgcg gccgcggcgg tgccggtttt ccgaccggtc
     1861 tcaaatggag tttcatgccg cgcaaggctc cgggtcagaa gtatgtcgtc tgcaattcgg
     1921 acgagtccga acccggtacc tgcaaggacc gtgaaatcct gatctataac ccgcatgcgc
     1981 tggtcgaggg catggcgatc gccggtttcg cgatcggggc caccgccggt tacaactaca
     2041 tgcgcggcga gttcatggac gagccctacc tgcatttcga gcaggcggtc aacgaggcct
     2101 acgccgccgg tctgctcggc aggaacgtcc agggttcggg catcgatttc gacctgtatc
     2161 cgtcgctcgg tgccggcgcc tacatctgcg gcgaggaaac cgcgctgctc gaatcgctgg
     2221 aaggcaagaa gggccagccg cgcttcaagc cgccgttccc ggcgaatttc ggtctgtacg
     2281 gccgtccgac gacgatcaac aacaccgagt cgctggcctc ggtgccgtac atcctgcgca
     2341 acggcgccca gtggttcgcc gatctcggca ttccgggggc cggtggctgc aagatcttct
     2401 cggtgtcggg acacgtcgcc aagcccggca atttcgagat ccgtctcggc acgccgttca
     2461 aggatctgct ggaactggcg ggcggggtct ggaaggggcg caagctcaag gccgtgatcc
     2521 ccggcggttc gtcggtgccg gtggtgccgg ccgagaccat gctcaaggtc aacatggact
     2581 atgactcgat ctcgaagggt gccggttcga tgctcggctc cggcgcggtc atcgtcatgg
     2641 acgagaccac cgacatggtc agggcgctgc agcgtctgtc gcgattctat ttttccgagt
     2701 cctgcggcca gtgcacgccg tgtcgcgaag gcacgggctg gctgtaccgg atgctgacgc
     2761 gcatcgtcga gggccagggc cgtcccgagg acatcgacct gctcgacagc gtggcgagca
     2821 agatcgaggg gcgcacgatc tgcgccctcg gcgatgccgc cgcgatgccg gtgcgcagct
     2881 tcgtcaagca ttaccgccac gagttcgcgt actacatcga gcacaagcgc agcatggtgc
     2941 ccggcgcgaa cgcggtcgcg gcctgacgcg ccatccaacc atcagagcgc cgggaacgag
     3001 agatgagtga ggatctggtc aatatcgagg tcaacggcac gccgctgcag gcccgcaagg
     3061 ggcagatgct gatcgaggtg accgacgcgg ccggcatcac ggtgccgcgc ttctgctacc
     3121 acaagaagct gtcgatcgcg gccaactgcc gcatgtgtct ggtcgaggtc gagaaggccc
     3181 cgaagccgct gccggcctgt gcaacgccgg tcatggacgg catgaaggtg cagacgcgct
     3241 cgccgaaagc actggctgcg cagaaaggca cgatggagtt cctgctgatc aatcatccgc
     3301 tcgactgccc gatctgcgat cagggcggcg agtgcgagct gcaggacgtg gcgatgggct
     3361 acggcggcga cgtctcgcgt tacgtcgagc gcaagcgcgt ggtcaaggac aaggacatcg
     3421 gtccgctggt cgcgaccgag atgacgcgct gcatccactg cacccgctgc gtgcgcttcg
     3481 gcgaggagat cgccggcctg cgcgagatgg gcgcgaccgg tcgtggcgag aacaccgaga
     3541 tcggcaccta cgtcaggaag agcctggcct cggagctctc cggcaacatc atcgacctct
     3601 gcccggtcgg cgcgctgacg gccaagccct cgcgctacac ggcgcgttcc tgggagatga
     3661 gccagcacgc cggcatcgcc gggcatgacg gcatcggctc cagcgtctgg catcacgtgc
     3721 gctacggcaa gatcgtgcgc accgtgccgc gcgagaacga agcggtcaac gaggtctggc
     3781 tgtccgaccg ggatcgcttc tcctacgaag gcctgtactc cagcgaccgt gccacgcagc
     3841 cgatggtgcg cgcagcggac ggtgcgctgg ccatcaccgg ctgggaggcc gcgctcgaag
     3901 cgaccgccgc cgggctgaag gcggtgatcg cgaagcacgg ggccgcccag gtcggcgtgc
     3961 tggtctcgcc gagtgcgacg cttgaggagc tgttccttgc gcagaagctc gcgcgcggtc
     4021 tcggcatcgc cagcatcgac catcgcctgc gtcagatcga tttcacggcc gctgccggtg
     4081 gttacccgtc gctcgggctc gcgctcgccg acgtgcccag gctcgatgcg gcgctggtca
     4141 tcggttcgaa cgtgcgcatg gaactgccgc tgttcgcaca ccgcctgcgt caggcctcgc
     4201 tgaagggcgg caaggtcgcg ttcctgaacc cggtgcgcta cgcgcagacg ctggacccgg
     4261 cggccgagtt cgtcgtctcg ccggccgcga tggtcggcac tctggcgcgc ctcgcggcgg
     4321 cggtggccgg cgtgaagggg gtgaacgtgc ccgcggcgct ggctgcggcc ggtgagccgt
     4381 ccgaggccga gcgcggctac gccgaaacgc tggccggtgc cggcagggcg gcgatcttcc
     4441 tcggccagct cgcgctctcg catccgcagt acgcgcagct gcaggccatt gccggtttcc
     4501 tggcgcgcag ctgcggggcg acggtcggct atctcgcccc cggtgccaat gccgccggtg
     4561 cgagcctcgc cggtgccctg ccggccgagg gcgggcagga tgcgcaggcg atgctcgcgg
     4621 ccggactcaa gggctacgtg ctgatcggca tcgagcccga gttcgactgc gcggcaccgg
     4681 cggtcgcggc cagggcgctt gccggggccg agttcgtcgt ggcgctgggc acgcacctcg
     4741 gcggcaccgt cgagcgcgag gccgatgtcg tgctgccggt cgcggcctgc gtcgaaacct
     4801 ccggcacctt cgtcaacctc gaaggccgct ggcaggactt ccaggccgtg gcgcgcgcgc
     4861 ccggtcaggt ccggcccggc tggaaggtgc tgcgcgtgct cggcaacctg ctcggcgtcg
     4921 ccggtttcga ccatctcagc tcggccgacg tggctgccga ggcgcgcagc gccgcggacg
     4981 gcatcgccgc gaccggagcg gtcgaggtcg cgcccgccga tgtgcagccg gccggggagg
     5041 ggctggcgtg catcgtcgag gtgccgatct acgcgatcga tccgatcgtg cgccgtgccg
     5101 ccgcgctgca ggcctcgccg ctcgccgtgt ccgccgaagt ccgcatcggc accgccggtg
     5161 ccgggcagtt cgggctgacc gccgatgcgc gtgccagcgt cgggcgcgac ggcaatgccg
     5221 tggtcctgcc cgtgaagatc gacgaccgca ttccggacgg ctgcgtctgg gtgccggccg
     5281 gcgtggaagc gaccggagcg ctcggtgcgg gcgccggtcc ggtcgaggtc cgcccggcct
     5341 gagcgccgac acccgacacc gaggggggga acgacggggg gcctgccgca cggcaggcac
     5401 cccggcgttg cgatccggaa cagaatgaca agctcgacag gacagagacc gtggacagcc
     5461 ttttgaccct ggtgctgata ctcctcaaga tcgtcgcgat cgtcgcgccg ctgatgctgt
     5521 cggtcgcgta tctgacctat gccgaacgca agatcatcgg ctacatccag gtgcgcatcg
     5581 gcccgaaccg cgtcggcccc aagggctggc tgcagccgat cgccgacgcc gtgaagctga
     5641 tgatgaagga aatcgtcatc ccgaccggcg cgaaccgctt cctgttcgtg ctggcgccgg
     5701 tgctgtcgat cggcccggcg ctcgcggcct gggcggcgat cccgctcggc gacggcatcg
     5761 tgctgtcgca catcgacgcc ggcctgctgt acctgctggc gatgacctcg ctcggcgtgt
     5821 acggcgtcat catcgccggc tgggcctcga actcgaagta ttccttcctc ggcgcgatgc
     5881 gctcggccgc gcagatcgtc tcgtatgaaa tcgcgatggg cttcgcgctg gtcggcgtgc
     5941 tgatggccgg tggcagcctg aacctcaacg agatcgtccg cgcgcagtcg ggcagcgtgc
     6001 tgcactggtt ctggctgccg ctgctgccgc tgttcatcgt gtatttcatc tccggcgtcg
     6061 ccgagaccaa ccgcgcgccg ttcgacgtgg ccgagggcga atccgaaatc gtcgccggct
     6121 tccacgtcga atactcgggc atgaccttcg cggtgttctt cctcgccgaa tacgcgaaca
     6181 tgatcctgat ctcggcgctc gccgcgacga tgttcctcgg cggctggctg tcgccgttcc
     6241 agggcatccc gctgctcggc gcgctgttca cgtgggtgcc cggggcggtg tggctgatcc
     6301 tgaagacggc cgtgttcctg ttcctgttcc tgtggctgcg tgcgaccttc ccgcgctatc
     6361 gctacgacca gatcatgcgt ctcggctgga aggtgttcat tccggtgacg atcgtgtggc
     6421 tggccgtggt cggcgtggcg aagtgggcgc atctggcgcc gtggttcgac tgaggccgga
     6481 ggggcgacaa agcaatggct gctctcaaac actatttccg cagtttcctg ctctgggagc
     6541 tgttcatcgg tctgcgcctg accggcaagt acctgttcgc gcgcaagatc acggtgcagt
     6601 acccggaaga gaagacgccg atgagcccgc gcttccgcgg cctgcacgcg ctgcgccgct
     6661 atccgaacgg cgaggagcgc tgcatcgcct gcaagctctg cgaggcggtc tgcccggcgc
     6721 tggcgatcac gatcgacatg cagcagcgcg aggacggcac gcgccggacc acgcgctacg
     6781 acatcgacct gttcaagtgc atcttctgcg gattctgcga ggagtcctgt ccggtcgatt
     6841 cgatcgtcga gacgcgcttc ttcgagtacg ccttcgagaa gcgtgaggaa tcgatcatga
     6901 ccaagccgaa gctgctcgcg cacggcgacc gcatggaagc ctcgatcgct gccgaccgcg
     6961 ccgccgacgc gcgctaccgc tgagccgcca cgggaaccga gatcatgggc ttcgagaaat
     7021 tcctgttcta cgtcttcgcc atcgtgctgg tgttcgcgtc ggtgcgggtc atcaccgtgc
     7081 gcaaccccgt gcatgcggcg ctgttcctgg tgctggcctt cttcaccagc gcggcgctct
     7141 ggctgctgct gcaggccgag ttcctggcca tcgcactggt gctcgtgtac gtcggcgccg
     7201 tcatggtgct gttcctgttc gtcgtgatga tgctcgacat caacgtcgcg ccgctgcggg
     7261 agggcttcat ccgcaacgca ccgctcggca tcgccgtcgc gacgatcatc gtcatcgaga
     7321 tggcgctggt gctgacctcg gatcgctccg gtctggtcag cggtgcgctg cagaactacg
     7381 cggccgatca cagcaacacc aaggaactcg gcagcgtcat gtacacgctc tacgcgtacc
     7441 cgttcgagat tgccgcactc atcctgctgg tcgccatcgt cgccgcgatc ggcctgacga
     7501 tgcgtcgccg tcagggcgtg cgctacgtcg atccggcaga gcaggtggcc gtgcggcgcg
     7561 agggccgggt gcgactcgtc aatctggcag ccgaaaagaa gagctgagag cgggtcgtgc
     7621 gcaccgccgg ttgcagccgc agatccatgc ggctgcaacg gtgtccggaa ttacagaaac
     7681 attgcgttag ggggatggat gatcgcgctt actgattacc tggtgctcgg ggcgatcctc
     7741 ttttgtctca gcgtggcggg cattttcctg aaccggaaaa atgtcatcat cctgctgatg
     7801 tccatcgagt tgatgctgct ggcggtcaat ttcaacttca tcgcgttttc ccgctttctc
     7861 ggcgacagcg ccgggcaggt gttcgtgttc ttcattctta ccgtggcggc cgcggaatcg
     7921 gcgatcggcc tcgcgatcct ggtggtgctg ttccgcaacc gccgcacgat caacgtcggc
     7981 gatctcgaca cgatgaaggg ttgagcgcat gcagaccgtc tatctctccc tcgtactcgc
     8041 gccgctgttc ggtgccatca tcgccggcct gttcggccgg cagatcggcc gcagcgccgc
     8101 gcactgggtc acgatcatcg gtgtcgcgat ctcgttcctg ctgtcgctcg cggtgttcaa
     8161 gcaccacgtc atcgacggcg cgccggccta caacgccacc gtctacacgt ggatggtcat
     8221 cgacggcatc cggctcgaag tcggattcct cgtcgattcg ctgaccgtca tgatgatcac
     8281 cgtcgtgacc ttcgtgtcgc tgatggtgca catctacacg atcggctaca tgcacgacga
     8341 tccgggctac cagcgcttct tcagctacat ctcgctgttc acgttctcga tgctgatgct
     8401 ggtcatgtcg aacaacttcc tgcagctgtt cttcggctgg gaagcggtcg gcgtcgtgtc
     8461 gtatctgctg atcggtttct ggttcaagcg cccgaccgcg atctacgcga acatgaaggc
     8521 cttcctcgtc aaccgcgtcg gcgatttcgg cttcctgctc ggcatcgccg cggtgctgat
     8581 gtatttcaac agcctcgatt accaggatgt cttccacgcc gcgccgctgt tcgcgaacgc
     8641 caagatgcag atcattcccg gcgtcgaatg gtcggtgatg accgtcacct gcatcctgct
     8701 gttcatcggt gcgatgggca aatccgcgca ggtgccgctg cacgtctggc tgccggattc
     8761 catggaaggc ccgacgccga tctcggcgct gatccacgcc gcgaccatgg tcacggccgg
     8821 catcttcatg gtctcgcgca tgtcgccgct gttcgagctg tcgacgacgg cgctgtcctt
     8881 cgtgctcgtg atcggttcga tcacggcgct gttcatgggc ttcctcggca tcatccagaa
     8941 cgacatcaag cgcgtcgtcg cctactcgac gctgtcgcag ctcggctaca tgaccgtcgc
     9001 gctcggcgtg tcggcctact cggcggccgt gttccacctg atgacgcacg ccttcttcaa
     9061 ggcgctgctg ttcctcggtg ccggctcggt gatcatcggc atgcaccacg agcaggacat
     9121 gcgcaagatg ggcggcctgc gcaagtacat gccgatcacc tggttcacgt cgctgctcgg
     9181 ctcgctggcg ctgatcggtt tcccgggctt ctcgggcttc tattcgaagg acagcatcat
     9241 cgaggccgtg catgcctcgc acatcccggg ttccggcttc gcgtacttcg ccgtcatcgt
     9301 cggcgtgttc gtgaccgcgt tctactcgtt ccggatgtac ttcctcgtgt tccacggcga
     9361 ggagcgcttc caccaccatg acgatgccca cggccacggc catgacgcgc accacgacga
     9421 cgaccacggg cacggccacc acggcggcac gccgcacgag tcgccgtggg tcgtcaccgt
     9481 gccgctgatc ctgctggcga tcccgtcggt gatcatcggt ggcaagacca tcgcgccgat
     9541 gctgttcggt gacttcttcc agggcgtcat ccacgtcgcg cacgagcacg acacgctgca
     9601 ccacctggcc agcgagttcc acagcgcgct cggcatggcc ttccacggcc tgctgacgct
     9661 gccgttctgg ctggcgatgg ccggcgtcgg cagtgcgtat tacatctaca tgaagcgccc
     9721 ggacatcgcc gagaagaccc gggcgaagtt cgcgccggtc taccggctgc tcgatcgcaa
     9781 gtacggtttc gacgacttca accaggcggt gtttgccgac ggcagcgtcg ttgccggcaa
     9841 ggcgctctgg aagtacggcg acgccggcct gatcgacggc gtggtcgtca acggctcggc
     9901 gaagaccatc ggctggctgg ccggcgtcgt gcgccacgtg cagtccgggt atctgtatca
     9961 ctacgccttc gcgatgatca tcggcctgct cgggctgctg acctggattg tcgtgcggtg
    10021 acagcggggg aatagaccga gatgctttcg caatttccac tcctgagtct cgtcatctgg
    10081 gcaccgatcc tcggcggcat cggcgtgctg ttcgtcggcg acgacagtcc ggccaaggcg
    10141 cggcgcctgg cgctggtcgc gtcgatcgcg accttcgtgc tcagcattcc gctgtggacg
    10201 aacttcagcc tcggcaccgc ggacatgcag ttccaggaac tgtatccgtg gatcccgggc
    10261 ttcaagatca actaccacct cggcgtcgac ggcttctcga tgccgctgat cctgctgacg
    10321 accttcatca ccgtgctggt cgtgatcgcc ggctgggaag tcatccagta ccgcgtcgcg
    10381 cagtacatgg cggccttcct gatcatggaa ggcctgatga tcggcgtgtt cgcggcgctc
    10441 gatgcgatcc tgttctacgt gttcttcgaa gcgatgctga tcccgatgtt cctggtcatc
    10501 ggcatctggg gcggtccgcg ccgggtgtac gcgacgatca agttcttcct gtacacgttc
    10561 ttcggctcgg tgttcatgct ggtcgcgctg atgtacatgt acttcaagtc cggcagcttc
    10621 gagatccttg ccttccacaa cctcaagctc ggcatgaccg agcaggtgct gatcttcctc
    10681 gccttcgtcg gtgcgttcgc ggtcaaggtg ccgatgttcc cggtgcacac ctggctgccc
    10741 gatgcgcacg tcgaggcgcc gaccggcggc tcggtgatcc tcgcggcgat cacgctgaag
    10801 atcggcggct acggcttcct gcgcttcgcg ctgccgatca cgccggacgc ctcggcctcg
    10861 ctcgactggc tgatcctcgg catgtcgctg atcgcggtgg tctacatcgg cctggtggcg
    10921 ctgatccagc aggacatgaa gaagctgatc gcgtactcgt cgatcgcgca catgggcttc
    10981 gtgacgctcg gcttcttcat cgtgttctcg atcctgcgcg cgaccgagtc gacctcgggt
    11041 gcggcgatgg gcatcggcgg cggcatggtg cagatggtct cgcacggctt catctccggc
    11101 gcgatgttcc tctgcgtcgg cgtgctctac gaccgcgtgc attcgcgcca gatcaaggac
    11161 tacggcggtg tcgccaacac gatgccggtg ttcgcggcct tcatggtgct gttcggcctg
    11221 gccaacgccg gcctgccggg cacctcgggc ttcgtcggcg agttcatggt catcctgtcg
    11281 agcttcaagg gcagcttctg gatcgcgttc ctcgcctcgt tcacgctgat cctcggcgcg
    11341 gcctacacgc tgtggctgat caagcgcgtc gtgttcggcg aggtcgtgca ccagcacgtc
    11401 gccgagctgc aggacgtgaa cgcccgcgaa ttcacgatcc tcgcggtgct ggccgtcgcg
    11461 gtgctcgcac tcggcctgtg gccgcagccg ctgaccgacg tgatgcaggc ctcgatcgag
    11521 cggctcgtgc agcacgtgct cgtgaccaag ctctgagccg gtgaaccccg gccgtcgccc
    11581 gccggcactc cggcgggcga cggtcgcaac cgcatacggt gatagggaat aacatgacct
    11641 tcgtcccgcc tgacctgatg cccgccctgc cggaaatctt cgtcgcgacg atggcctgcc
    11701 tcgtgctggt catcgacgtg tatctgcccg aggcccggcg cacgacgacg ctgtggctgt
    11761 cgatcgccac gctgctgttt gccgccctgc tgacgctggc gacggccccg gaagcccgcg
    11821 tgcccggctt ccacggccac ttcgtgcgcg acgcgatggg cgacgtgctg aagatcggtg
    11881 tctatctgtc gatggccgcc gcgctgctgt attcgcgtga ctacctgcgc aaccgcacgc
    11941 tgttcaaggg cgagtactac gtgctcgcgc tgttcgcggt gctcggcatg atggtgatga
    12001 tctcggccgc gagcttcctg accgtgtatc tcggcctcga actgctgtcg ctgtcgctgt
    12061 acgcgctggt cgccttcgat cgcgaggacg gccgctgctc ggaatcggcg atgaagtact
    12121 tcgtgctcgg tgcgctcgcc tccggcatgc tgctgtacgg catctcgatg atgtacggcg
    12181 ccaccggcag cctgaacctc gcgagcgtcg cgcaggcgat cgccggcaag ggcgacaaga
    12241 ccctcgtgct catcttcggc accgccttcc tggtcgtcgg cctcgcgttc aagctcggcg
    12301 ccgtgccctt ccacatgtgg ctgccggatg tctatcaggg cgcgccgacc tcggtgacga
    12361 tgatcgtcgc ctcggcaccg aagctcgcgg ccttcgcgct gacgatgcgc atcctggtcg
    12421 agggcgtcgc gccgctcggg gccgactggc agcagatgct gatggtgctc gccgcgctgt
    12481 cgatcgccgc cggcaacgtc atcgccatcg cccagaccaa catcaagcgc ctgctcgcgt
    12541 attcgacgat ctcgcacgtc ggcttcatcc tgctcggctt cgttgccggc acggccgagg
    12601 gctacgcggc gtcgatgttc tacgcgctga cctacgtgct gatgacggtc ggctccttcg
    12661 gcctgctgat cctctgcggt cgcgccggcg tcgagatcga gaacatctcg gatctcaagg
    12721 gcctcaacga ccgcagcccg tggctggcgt tcatgatgct ggtgctgatg atgtcgaccg
    12781 ccggcattcc gttcctggtc ggcttctacg ccaagttcgc cgtgctgcag gcggtcgttg
    12841 cgcagggctt cgtcgggctc gcgatcttcg cggtgatcct gtcggtgatc ggcgcgttct
    12901 actacctgcg cgtcgtcaag gtcatgtact tcgacaaggc cgaggacgac accaccctca
    12961 gcgtgtcggc cgatgcgcag gtgctgatgg gcgccaacgg tctggcggtg atgctgctcg
    13021 gcgtctatcc gtcggggctg atggcgctct gcagcgccgc gatccactga cggcacggac
    13081 gagggcggca atgggtcagg atgcgctggt caccctgtgg ctgctgcttg cagcggtggc
    13141 ggtcaacctg ccctggctca gcgaccgcct gctgctggtg cgcagcgccg gggccgccgg
    13201 caagccgacc tggctgcgct ggctggaatg gctgctgctc gccgtgctgg tcacggccgc
    13261 gggccttgcc agcgagtgga aggcgaccgg cgagcatcat ccgcaggact gggagttcta
    13321 cgtcgtcgcg atctgcctgt cgctggtcgc ggcgatgccg ggcttcgtct atcgctacca
    13381 gtggctgccg ctcgccagcc ggcggacgcg cggcttgtag gggcgggcgg ctgttgttat
    13441 agtccgcggc tcgcggtgca gggggcagct tcaggccgcc tgcaccgggt ccggttgccg
    13501 gacacgggtc cttgacccgc ccgccacctc agtcgtgaca ccgggcccct agcgggcctt
    13561 tttcttaccc gcgttccggc gtacgagtga cggcatgcgg gtgctcagga caggcagtca
    13621 ggaatttcag tgggcctcgg gcccactttt catttgggta tcgatcgatg cgcgccgatc
    13681 cttacaagct gcgggaactg ctcgggccgt cgattgccgg cctcggctgc gaactcgtcg
    13741 gcatcgagta tcacccgaac tcggcgaacg ccctgctgcg catctatatc gatcgcgccg
    13801 agggcgtgac ggtcgatgac tgcgagcgtg tcagccgtca ggtcagcgcg ctgctggatg
    13861 tggccgaccc ggtgcccggg cactacacgc tggaagtgtc gtcacccggt ctggaccggc
    13921 cgctgttcga gccggagcat ttccagcgtt tcaccggccg tcaggcgcgt gtgcagctcg
    13981 gcatggcgct cgatggccgg cggcgcttcg tcggcgagat cctcggcctg cgtgacggcg
    14041 agctgttgct gcgctgcgag ggcacggaag tcgcgattcc cctggaccgc atcgaaaagg
    14101 cacgactggt tcccgaattc tgaatcgggg agatgggata caggcgctat gagcaaagag
    14161 attcttctgg tggtcgatgc cgtctccaac gagaagggtg tggcgaagac cgtgattttc
    14221 gaagcaatcg aggccgcgct ggcctcggct gccaagaagc gctacgccgg cgatccggac
    14281 gtgcgcgtcg agatcaaccg caagaccggc gagtaccaca ccttccgctg ctgggaggtg
    14341 ctggccgatg acatcgaacc cgaatttccc gatcgccagc tgccgctcgg gcgtgccgtc
    14401 gaactcgacc cggccgccga gaccggggcc ctgatcaccg agccgctcga gaacgccgcc
    14461 ttcggccgca tcgccgcgca gaccgccaag caggtcatcg tgcagaaggt gcgcgaggcc
    14521 gagcgggcgc aggtcgtcga tcagttcctg ccgcgccgcg gccagctgat cagcggggtg
    14581 gtcaagcgcg tcgagcgcgg caacgtgctc gtcgatctcg gtggcaacat cgaggccatg
    14641 gtcgcgcgcg aggacatgat cgcgcgcgag ccggtgcgca acggcgaccg catccgcggc
    14701 tggctgaagg acgtgcgcca cgagccgcgc ggcccgcaga tcttcctgag ccgcacggcg
    14761 ccggagttcc tgatcgagct gttccggctc gaagtcccgg aggtcggtca gggcgtcatc
    14821 gagatcaagg gcgcggcgcg cgatccgggc gtgcgcgcca agatcgcggt caagaccaac
    14881 gaccagcgca tcgatccggt cggggcctgc gtcggcatgc gcggctcgcg cgtgcagagc
    14941 gtctcgaccg aactggccgg cgagcgcatc gacatcgtgc tctggaacga caacccggcg
    15001 cagttcgtga tcaatgccat gtcgccggcc gaggtgatct cgatcgtcgt cgacgaggac
    15061 agtcacgcga tggacatcgc ggtcgccgag gaaaagctgt cgcaggccat cggccgcggc
    15121 ggtcagaacg tgcgcctggc ctcggatctg accggctggg agctcaacgt catgacgctc
    15181 gcgcaggccg aggccaagag tggccaggaa gcgcaggcgg tcctgcagat gttcatggac
    15241 atgctgtcgg tcgatgccga cgtggcggaa atcctggtgc gcgaaggttt ttcaacgctg
    15301 gaggaagtgg cctacgtgcc tgtacacgag atgctggagg tcgaggaatt cgaccaggaa
    15361 atcgtcgacg aactgcgcgc ccgggcgcgc gacgcgctgc tgacgcgcgc gatcgcagcc
    15421 gaggagcagg tcggcgatgc cgaaccggcg gaagatctgc ggcgcatgga gggcatggac
    15481 gaggatctgg cgttcctgct ggccggcaag ggtgtgcgca cgcaggaaga tttggcggag
    15541 ctgtccgtgg atgatctgat cgacatggcg gatatcgacg aagcacgtgc cgcgcaactc
    15601 atcatgacgg cgcgtgcgcc ctggttcagc ggcgaccagg gctagcaggt cgggggtacg
    15661 gaattatgac ggaagtaacg gtcaagcagt ttgcagcggt cgtcggcaca ccggtcgacc
    15721 ggctgctgga gcagtttgcg gaagcaggca ttcaggtcgg cggtgcggat tcgagcatca
    15781 ccgaccagca gaaaatggaa ttgctggcgc acctgcgcaa gagccacggg cgcaaggagt
    15841 ccctggcggt cgccgagccg aagaagatca cgctgcagcg caagacgcac agcgagatca
    15901 aggtcgcggc agcccccggg cagacgaaga cggtcagcgt cgaggtgcgc aagaagcgca
    15961 cctacgtcaa gcgcgagccg ctgccgggtg aaaccgtgcc ggtgaccgag acggcgccgg
    16021 ccgaggagtt cgcggatgac gcggcggcga tcgccgagac cttcgccgcc gaagcgcccg
    16081 catccaccga ggcctacacc gaagccgtgc cggagccggt ggccgagacc gggtcgtcca
    16141 cggcccgcgc cacgccggcc cgtccgacgg tccagcgcgc cgcaccggtc gagcgccggc
    16201 cggttgcgcc gtcgacctcg gtgctcgccc gtccgcgtgc cgatcagaac agggctcgtg
    16261 ccaacgaatc cctggagctg ccgcgcgtcg acaacacgcg gccgaaggtg gtcggcgtcg
    16321 tctccaagaa gcaggtggcg cgcgatgacg cggccgccgc agc
//
LOCUS       DAOQIE010000039        16331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1667_length_16331_cov_7.756574, whole genome shotgun sequence.
ACCESSION   DAOQIE010000039 DAOQIE010000000
VERSION     DAOQIE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16331
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1667_length_16331_cov_7.756574"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PK144_04090"
     CDS             <1..877
                     /locus_tag="PK144_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupU protein"
                     /protein_id="HNF64939.1"
                     /translation="HPSLSEASGAEVIALLEAIAAGERPLDCLCIEGALLRGPNGSGR
                     FHVLGGTGVPTIDWVRRLAARARCTVAVGSCAVSGGFSACAPNPTEACGLQFEDARRG
                     GALGADWRSPSGLPVIALGGCAVHPDWVLETLAALALGELDAGGLDAFGRPRAIYERL
                     VHHGCPRNEYYEYKASAEKPSDLGCLMEHLGCRGTQAHADCNERVWNGGGSCTRAGFA
                     CIDCVAPGFQNPGHAFQRTPKLGGIPVGLPTDMPKAWFVALASLAKAATPARVRENAV
                     ADHPVVPPKMHVRKP"
     gene            874..2337
                     /locus_tag="PK144_04095"
     CDS             874..2337
                     /locus_tag="PK144_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNF64940.1"
                     /translation="MSRLVVGPFNRVEGDLEVRLDLADGRVRSAEVSAPLYRGFEAML
                     IGRVPEDALAIVPRLCGICSVSQSAAAAFALADAAGISAPPNGQLAANLVLACENLAD
                     HLAHFYLFFMPDFARPAYAQRPWFPAVHARFAAGGGTAGPEAMAARTAFFHIAGLLAG
                     KWPHTLALAPGGTTRAPGTGEKIRLRLVLAGFRQFLETRLFGCALEAFAGLATPAQLA
                     GWQHAAAPESCDLRLFLHAADELGLAGLGRLDLPLMSFGAYVSEADGRLFRAGLIADG
                     ALHALDPGDIREDTSHAWLRSAAPLPPAAGRTEPDIDVPGAYTWCKAPRLSGRPVEVG
                     ALARQLVDGQPLLRALHGGNGRNGGTNVVARVLARAVELARVVPAMERWSSLLEAGAP
                     CCVPAPLPAGGSGIGLIEAARGSLGHWLGIDGGRIRHYQIIAPTTWNFSPRSADGVPG
                     PLEQALIGTAVEPGETVPLAVQHVVRSFDPCMSCTVH"
     gene            complement(2449..2634)
                     /locus_tag="PK144_04100"
     CDS             complement(2449..2634)
                     /locus_tag="PK144_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64941.1"
                     /translation="MANFLKRLHAGFKRLICPVLAHDWQFENIHRDGHCTAVCRRCGR
                     REPALVLTHEPHPGVHA"
     gene            2930..3709
                     /locus_tag="PK144_04105"
     CDS             2930..3709
                     /locus_tag="PK144_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNF64942.1"
                     /translation="MSLTRRRFLKYAAASASAAALARLPTASAATAAVPVAAIALDGF
                     TTFDPRPLAALAEQMFPDGAGKALTLAWRTRQFEYTWLRTLMNRYADFWQVTGEALDF
                     AAAQTGLKLGGEQRDRLMQGWLELRAWPDAADALRALKAEGLRLAFLSNFTPAMLDRA
                     VAGSGLDGLFDEHLSTDRVRAFKPDPRAYAMAEGFWKLPREAIAFAAFGGWDAAGARA
                     YGYPTFWVNRAGVPAERLDTLADASGATLTELAAWVRMTRG"
     gene            complement(3725..4831)
                     /locus_tag="PK144_04110"
     CDS             complement(3725..4831)
                     /locus_tag="PK144_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HNF64943.1"
                     /translation="MSALLRGEKPLLSAIAVILIAYPLEHTLIDSGRSMALIASGVLI
                     AVILSVALRVAHHAEILADRYGEPYGTMILTISAVLVEVVILAILLSHNPSPTLVRDT
                     IYSALMLDINGILGLAALLGGLRHGVQAYNVDSGNTYLTMILCAVGVSMIVPEFIPHM
                     TWKAYSIFNILVMLLLYALFLRLQTGQYAGFFSYRYAHRAGQSPQELHAESEAAHDPS
                     AVRNSAALLVTGLVLIGALSEVMAKTIDVSLAGSGMPVIVPGMLVALISASPEIMTAL
                     RAALEDRMQPVVNIALGASLSTVILTVPVIEAIALLSGQPIEMALTAPQTVMMVLTLL
                     AATINLHDGETNAIEGTAHFVLFLTFLMLAALGV"
     gene            5182..5682
                     /locus_tag="PK144_04115"
     CDS             5182..5682
                     /locus_tag="PK144_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNF64944.1"
                     /translation="MIYLAGPAVFHPDAAGIGRRLRALCTAAGCEGLFPLDAEVPGDA
                     GRGPAQAAAILQANLALLERADAVLADCTPFRGPSLDPGTAFEIGYAVARGKPVAGWS
                     ETAGAYRLRVAQAVRCASGWRDAGGWVIEDFGLPDNLMISASLVAMADDPAGALAQLL
                     AHLRAR"
     gene            complement(5694..5927)
                     /locus_tag="PK144_04120"
     CDS             complement(5694..5927)
                     /locus_tag="PK144_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64945.1"
                     /translation="MQSCYRRWESRDRWYEASIGTDLFGQLTLTCRWGALRSARGGMQ
                     CRPLTGAAEAGRLLQALARRRERHGYRIVAVLA"
     gene            6194..7006
                     /locus_tag="PK144_04125"
     CDS             6194..7006
                     /locus_tag="PK144_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64946.1"
                     /translation="MKATDLIDDLNRRFADDDYGRRAPAALQRSSDGAVGSFQGLRLS
                     TGVETLFAADGGISGHQAVTVVRGPHGRPLSAWVPYALALDAQAIGWLDRLVRTLHVF
                     NARAVLSLGRLWLAVHPLHLGAISGEFGAVFANLLARMRVHPSQIVLEIADADRMPRS
                     RLMQALSGFRTHGFGTACVLRGDDPGAFEQVLSLHPDAVKLCRSHLYAARASLLGRER
                     LARRIAAIRAAGAHAWLRGVDDLPSTRLARALDADGWQSGLLDARMLGGHGG"
     gene            complement(7042..7827)
                     /locus_tag="PK144_04130"
     CDS             complement(7042..7827)
                     /locus_tag="PK144_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNF64947.1"
                     /translation="MSASPLPLIPSIPSSDPERRFGGIARLYGSDALARLQAARVVVV
                     GVGGVGSWAVEALARSAIGTLRIIDLDIVHESNVNRQLPALDGEFGRAKVEVLAERIG
                     RINPACTVEAVEDFLTPENAAGLLAGADAVLDAIDNVRAKAALIACCRSAHLPLVVSG
                     GAGGKQDPGRIRLSDLAQTEQDPMLAKLRQRLRKEYGFPRDPARRFGIDCVWSPEPVL
                     RPRSCAGDAGLNCAGYGASMCMTASFGLRAAARLIERLSASGR"
     gene            complement(7924..8343)
                     /locus_tag="PK144_04135"
     CDS             complement(7924..8343)
                     /locus_tag="PK144_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF64948.1"
                     /translation="MSRTTAGTEILQNAVSVPVQRVVSGAAALMMRGMSAALNPEQTA
                     MSMHALHAMPGPQGHGYDSVHVPHAVITQVVVYGDTPLRAWREYLGISRETVAERMGV
                     SARVIQAWEENPLTPPRRSALVPLARALGIDVSRFDL"
     gene            8587..8973
                     /locus_tag="PK144_04140"
     CDS             8587..8973
                     /locus_tag="PK144_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64949.1"
                     /translation="MTPRRARVVESKIIIPICPVNGRFSAVVQTVLLHPTPEGVHGAM
                     PIRADLSHIDLAAMSLPDLHRLVDKIEKAIEIKLFERGAQRALAVFSAHAPTPAAAPP
                     VGRGGNRDVRRTGRERSGRDSGLRRR"
     gene            complement(9161..9811)
                     /locus_tag="PK144_04145"
     CDS             complement(9161..9811)
                     /locus_tag="PK144_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNF64950.1"
                     /translation="MPELLTIAGVQQAERQLSRHPGLAELISRHGPCPITPLPGVQPW
                     LALCSAVIHQQLAGRAAAAIEARFRSRISTAPVPDGVLALSDDDLRGTGLSRQKAAAI
                     RDIAARAASGFLPDRTEADRLTDDELVSRLTAARGVGRWTVEMFLIFTLGRPDVWPVD
                     DYGVRRGWCVIEANPGLPRARELLHWGEAWRPWRSVLAWYLWRAAEDGSTGTPAAQ"
     gene            10007..12805
                     /gene="prsT"
                     /locus_tag="PK144_04150"
     CDS             10007..12805
                     /gene="prsT"
                     /locus_tag="PK144_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNF64951.1"
                     /translation="MLHRFRRGLSAAAVAITLAGTGTGVAGTSADPEYLQRAEEHRKA
                     HDLPAAIIELKNALAEAPGDARVRLLLGSLYLEQGDGAAAEKELRLARRLGVPLPRLM
                     AGLAEAIYLQRAWQRLVDDVQPLTGMSAREVADVRALRGEAQFALRRNDEARTSLESA
                     LRTDPAAVRALLGSARLALSEQRHDQARVWLQRAVDQSLSPQLANDAWSQFGDLEVAT
                     RHLPAADAAYTRAIDTGQSHLGVVLRRGLVRVELQDYDGAAADLNRVAGRAPDHTLVH
                     YGFGLMAYRQGHYAEAAASFSRARQRAPDNADASYYLAASQLALGQLDPAIDGLSRFL
                     AEHPESEVTARLLGNAYLRRGDANAAAALIQPLLERQPDDAALLDLLGRITLIQGDAA
                     RSRELLARATELAPSSAFGRYAYGMSLLAAGDQNRGLEELQKAVELDPHEHGLEFGLS
                     VSLLRAGHFDEAIAVARRLQQSLPDQAEPLVLEGIAQAARQQDAEARAAFRRALALEP
                     GHPRAAGALAALDLGSGDIDGARGWYRQILAHHPGHAPTLMQLARLEARQGQGEAYAE
                     TLREAIAADPHVPQPYVLLARWQLRQGQPLLAVGTLVAVRAEFPEYPPLLLTLGLAQG
                     ANGESADAIGTLRRLIELQPQSAEARYALVTAHAQAGDAAGLRGTLAQALAVDNRRPE
                     ALPLLERLVGLAGSASEAEDWLEALLRDRPDDEIVLDFIGQRRYRQYGAPTALTYYAQ
                     LQRRQPQAALWVLREAELRSGNREAAQAQALLERWLQAHPDDVQARFALGLIHLDADR
                     RAPARAAFEAVLERRPDQLAALNNLAWLLREDDPQRALDYANRCLQLRPADPRLMDTL
                     GEVLIAQHQYARAAEVLAKAVARAPQLGSARYHLALALSLDGRRDEARELLKSLLGND
                     QPFSERAEAQSLLAQIGS"
     gene            12898..15687
                     /gene="prsT"
                     /locus_tag="PK144_04155"
     CDS             12898..15687
                     /gene="prsT"
                     /locus_tag="PK144_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNF64952.1"
                     /translation="MMWNTRMRPLAAALLLLAGSATAAADLARELDKAEQLRAQGQLG
                     AAIIELKNALQDEPDYGPGRRLLGVLYVELGNGAEAEKELLRARRLKVRETGIALPLA
                     EALLLQREYQRLLESVQPVSGMSMRDVADLRALRGEALLALGRQGDAEAAVKSALATD
                     PAAPRAQIAEARLALAARHPDTARQRLQTVLSAPLPQPVEHIAQALLGDVEIMARRLP
                     EAAKAYDRAIATGRPHQALAIQLKRGLLRIDLGDPDGARADLGAVNARLPDLPLPHFG
                     FGLLAYRQGRYADALDSFERALQRDHENPDIHAKIAATRIALGQREQAVTDLEKFLQE
                     HPDSDPILRLLATIRLQQGDTAGAQKLLRHVLELHPDDNAVLDMLGAIALARGEAREG
                     EPLLRRSVERDPDSAAARYRLGLGLLALGDRENGLRELAQATQLQPGQERYEYALALG
                     LLRSGQHAEAVAAAQRLQALWPDRNEPLLLEGIARASALDTEGASAAFERVLARSPGD
                     PAASGGLATLDLQQGRPDKARSRYRAVLEQHPGHGATLLELAALELRLDNPEAARRAL
                     EQAVAADPEALEPRLRLARLLGREGRHAAALELLEAVHARHADDVQLLALLGAAKTAL
                     GDAGGGRAAYRRLLDLDPKSATGLYGYASACAAGSDDAGLRAALRPALAADPGNALAQ
                     ALLARLQELAPGEAAARAWFEELNQALPDNLALLEFEGRRIERQQGRQPAAIYYGGLR
                     RRQPHEQLWALREVELWQQAKEPAQARSVLVDWLRWHPDDDTVRAQLAAMMLAAGQNA
                     DAKAELERIVADNPTRVSALNNLAWLLRKDDPRRALRLAERAVRLRPEPRILETLGEV
                     LIAGGEYVRATEVLQYAALRAPQQPSIRYQLAVALAGQQRKEDARRLLKELLAGDTRF
                     AERSDAAALLKKLGG"
     gene            15763..>16331
                     /locus_tag="PK144_04160"
     CDS             15763..>16331
                     /locus_tag="PK144_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64953.1"
                     /translation="MPRTALYRTRAAVCACVLILSGTGAQADDDVLTAAGRKLEAGQV
                     SAAVIDIKNRLQQRPGDGAARRLLGIAYVRLGDGANAEQELMRAAQLGVPAGELAVPL
                     AEALAVQQQWQALLDRAIVQPNMRTPELAELRALRATAHQALGQTGQARLQFEAAVAT
                     DAASARAQLGLAQFDLQAGRWPAARTRLQT"
BASE COUNT         2404 a         5847 c         5680 g         2400 t
ORIGIN      
        1 gcatccgtcg ctgtcggagg cgagcggtgc cgaggtcatc gcgctgctcg aagccatcgc
       61 cgccggcgaa cgcccgctcg attgtctctg catcgaaggc gcgctgctgc gcggaccgaa
      121 cggcagcggg cgcttccacg tgctcggtgg caccggcgtg ccgaccatcg actgggtgcg
      181 caggctcgcg gcgcgcgccc gctgcacggt cgcggtcggc agctgcgcgg tctcgggcgg
      241 cttcagcgcc tgcgcgccga acccgaccga agcctgcggc ctgcagttcg aggatgcccg
      301 ccgcggcggt gcgctcggcg ccgactggcg ctcgccatcc ggcttgccgg tcatcgcact
      361 cggcggctgt gccgtgcatc cggactgggt gctggaaacc ctggccgcgc tcgcgctcgg
      421 cgagctcgac gccggcggcc tcgatgcctt cggccggccg cgcgcgatct acgagcggct
      481 cgtgcatcac ggctgcccgc gcaacgagta ctacgaatac aaggcgagcg ccgagaagcc
      541 gtccgatctc ggctgcctga tggaacacct cggctgccgc ggcacgcagg cgcacgccga
      601 ctgcaacgag cgcgtctgga acggcggcgg ttcgtgcacg cgcgccggct tcgcctgcat
      661 cgactgcgtc gcaccgggct tccagaaccc cggtcacgcc ttccagcgca cgcccaagct
      721 cggcggcatc ccggtcggcc tgccgaccga catgccgaag gcctggttcg tcgcgctcgc
      781 ctcgctcgcc aaggccgcga ccccggcgcg cgtgcgcgaa aacgctgtcg ccgaccatcc
      841 ggtcgtgccg ccgaagatgc acgtgcgcaa gccgtgagcc ggctggtcgt cggcccgttc
      901 aaccgcgtcg agggcgatct cgaagtgcgc ctcgaccttg ccgacggccg cgtgcgctcg
      961 gccgaggttt cggcaccgct gtatcgcggc ttcgaagcga tgctgatcgg gcgcgtgccc
     1021 gaggacgcgc tggcgatcgt gccgcggctc tgcggcatct gctcggtgtc gcagtccgcg
     1081 gccgcggcct tcgcgctcgc tgatgccgcc ggcatcagcg ccccgccgaa cggccagctc
     1141 gccgccaacc tcgtgctggc ctgcgagaac ctcgccgatc acctcgcgca tttctatctg
     1201 ttcttcatgc ccgacttcgc gcgcccggcc tacgcgcagc ggccgtggtt cccggccgtg
     1261 cacgcgcgct tcgccgccgg cggcggcacg gccggcccgg aagcgatggc cgcgcgcacg
     1321 gccttcttcc acatcgccgg cctgctcgcc ggcaaatggc cgcacacgct ggcgctcgca
     1381 cccggcggca cgacgcgcgc cccgggcacg ggcgagaaga tccggctgcg gctggtgctc
     1441 gccggcttcc ggcagtttct ggagacgcgg ctgttcggct gtgcgctcga agccttcgcc
     1501 gggctcgcca ccccggcgca gctcgccggc tggcagcacg cggccgcgcc ggaaagctgc
     1561 gatctgcgcc tgttcctgca cgccgccgac gagctcggcc tcgccggcct cgggcgcctc
     1621 gacctgccgc tgatgagttt cggggcctac gtcagtgagg ccgacggccg gctgttccgg
     1681 gccggactga tcgccgatgg cgcgctgcac gccctcgacc ccggcgacat ccgcgaggac
     1741 accagtcatg catggctgcg ctcagcggcg cctctgccgc cggccgccgg tcgcaccgag
     1801 cccgatatcg acgtgcccgg ggcctacacc tggtgcaagg caccgcgcct gtccggccgg
     1861 ccggtcgagg tcggggcgct ggcacgccag ctcgtcgacg gccagccgct gctgcgcgcg
     1921 ctgcacggcg gcaacggccg caatggcggg acaaatgtcg tcgcgcgcgt gctcgcgcgt
     1981 gccgtcgaac tcgcacgcgt ggtgccggcg atggaacgct ggagcagcct gctcgaagcc
     2041 ggcgccccct gctgcgtgcc ggcaccgctg ccggccgggg gttcgggcat cggactgatc
     2101 gaggcggcgc gcggcagtct cggccactgg ctcggcatcg acggcgggcg catccgccac
     2161 taccagatca tcgcgccgac cacgtggaac ttctcgccgc gttcggccga cggcgtgccg
     2221 ggaccgctcg aacaggcctt gatcggcaca gcggtcgagc ccggcgaaac cgtgccgctt
     2281 gcggtgcagc acgtcgtgcg ctcgttcgat ccatgcatga gttgcacggt ccactgatac
     2341 ggccactgac acgaactgct gacacgacga cgcccggtgg cttgcacggc catccggacg
     2401 tcagtggaca ggcgctgtca ggtttcgggc agggcgggga gcgagggatc aggcgtggac
     2461 gcctggatga ggctcgtgcg tcagcaccag ggcgggttca cgtcgtccgc agcggcggca
     2521 gacggcggtg cagtgaccat cgcggtggat gttttcgaat tgccagtcat gcgccagaac
     2581 cgggcagatc aggcgcttga agccggcatg aagccgcttg aggaagtttg ccatgctgtg
     2641 tctccgctac atcctggacc gcacgtccat gggcggaatc ggtcaagcgc cgatgaagct
     2701 agaagcccgt tcgggaagca gcaagtgctt gaaatcctta ttgacattaa ctttgcgtca
     2761 ctttctgccc attctgcttt gtccatacgg caagcaccga atccgcaagg catttccatc
     2821 ggcgacgcgg atattccggc aattttccat tgacgtttcg ttacatccgg catccgggct
     2881 ccgcgtggct gcgtagccag cgacacccct catccagccg gaatccgcca tgtccctcac
     2941 ccgtcgccgc ttcctgaagt acgccgccgc cagcgccagc gcagcggcac tcgcccgcct
     3001 gccgacggct tctgccgcca cggccgcggt gccggtcgct gccatcgccc tcgacggctt
     3061 cacgaccttc gatccacgcc cgctggccgc cctggccgag cagatgtttc cggatggagc
     3121 gggcaaggcc ctgacgcttg cgtggcgcac gcgccagttc gaatacacct ggctgcgcac
     3181 gctgatgaac cgctacgcgg atttctggca ggtcaccggg gaagcgctgg acttcgctgc
     3241 agcgcagacg ggcctgaagc tcggcggcga acagcgggat cggctgatgc agggctggct
     3301 ggagctgcgc gcctggccgg atgcggctga cgcgctgcgg gcgctgaagg cagagggcct
     3361 gcggctcgcg ttcctgtcga acttcacgcc ggcgatgctc gatcgggccg tcgccggcag
     3421 cggcctcgac ggcctgttcg acgagcacct gagcaccgat cgcgtgcgcg ccttcaagcc
     3481 cgatccgcgc gcgtacgcga tggctgaggg tttctggaag ctgccgcgcg aggccatcgc
     3541 attcgcggcc ttcggaggct gggacgcagc cggggcgcgt gcgtacggct atccgacctt
     3601 ctgggtcaac cgcgccggcg taccggccga acggctcgac acgctcgccg atgccagcgg
     3661 tgcaacgctg accgagctgg cggcctgggt gcggatgacg cgcggctgat accggcagcc
     3721 ggcatcagac cccgagcgcc gccagcatca ggaaggtcag gaacagcacg aagtgcgccg
     3781 tgccctcgat ggcgttggtc tcgccgtcgt gcaggttgat ggtcgcggcg agcagcgtca
     3841 gcaccatcat caccgtctgc ggggcggtca gtgccatctc gatcggctgg ccgctcagca
     3901 gcgcgatggc ctcgatcacc ggcacggtca ggatcaccgt cgacagcgag gcgccgagcg
     3961 cgatgttgac gaccggctgc atgcggtctt cgagagcggc gcgcaatgcg gtcatgatct
     4021 ccgggctcgc cgagatcagc gcgaccagca tgccgggcac gatcaccggc atgccgctgc
     4081 cggcgaggct cacgtcgatg gtcttggcca tcacttccga cagcgccccg atcagcacca
     4141 ggccggtgac cagcagcgcc gccgagttgc gcacggcgga cggatcgtgt gcggcttcgg
     4201 attcggcgtg cagttcctgc ggtgactgac cggcgcggtg ggcgtagcgg tagctgaaga
     4261 aaccggcgta ctgaccggtc tgcaggcgca ggaacagcgc atacagcagc agcatgacca
     4321 ggatgttgaa gatcgaataa gccttccagg tcatgtgcgg aatgaactcg ggcacgatca
     4381 tcgacacgcc gaccgcgcac aggatcatgg tcagataggt gttgccggaa tcgacgttgt
     4441 aggcctgcac gccatggcgc agtccgccga gcagggcggc aaggccgagg atgccgttga
     4501 tgtcgagcat cagcgccgaa tagatggtgt cgcgcaccag cgtcggcgag ggattgtgcg
     4561 acagcaggat ggcgaggatc acgacctcga ccagcacggc gctgatggtc aggatcatcg
     4621 tgccgtaggg ttcgccgtag cgatcggcca ggatctcggc gtgatgtgcg acccgcaggg
     4681 cgacgctgag gatcacggcg atcagcacgc ccgatgcgat cagcgccatg ctgcggccgc
     4741 tgtcgatcag cgtgtgttcg agcggatagg cgatcaggat gacggcgatc gcgctgagca
     4801 gcggcttttc gccgcgcagg agagcggaca gggatacggg cgacatgcgc agcgcctttc
     4861 ggtcgggcag gggagggaag tgactgcgcg cgagtatagg agcggccccg gccggcccgc
     4921 tggcgggagc cgcttccgga aattttgccc gtcaacccgg acaacccttg acgcgctgca
     4981 acatcgcgtc catattgcaa tgcaacatcc gctgcgtcgg gtgttacctc tcctcctcct
     5041 tttgggccgg tcctcttccg gccctttttt tatgcccgcc tccctgcccg ccgggcccct
     5101 gacaacgctc gcgctgccgg ctacacccgg gcgggtccgt gcgcgcttga ggagccgatc
     5161 cccatgaagc cagagtgtcc cgtgatctat ctcgccggac ccgccgtgtt ccatccggac
     5221 gccgccggga tcggccgacg tctgcgggca ctgtgcacgg ccgccggttg cgaggggctg
     5281 tttccgcttg acgccgaagt gcccggcgac gccgggcgcg gtcccgcgca ggcggcagcc
     5341 atcctgcaag ccaacctcgc gctgctcgaa cgggccgatg ccgtgctggc cgactgcacc
     5401 ccgtttcgcg gcccgtcgct ggacccgggc acggcgttcg agatcggcta tgccgtcgcg
     5461 cgcggcaagc cggtggccgg atggtcggaa acggccgggg cctaccggct gcgcgtggcg
     5521 caggcagtcc gctgcgcgtc gggctggcgg gatgccgggg gatgggtgat cgaggatttc
     5581 ggactgcccg acaacctgat gatcagcgcg agcctcgtgg cgatggcgga tgatccggcc
     5641 ggcgcgctgg cgcaactgct ggcgcacctg cgtgcccgct gaagccaccg ggctcaggcg
     5701 aggactgcga cgatccggta accgtgtcgc tcgcgccgcc gggccagggc ctgcagcagg
     5761 cgtccggctt cggctgcacc ggtgagcggc cggcattgca tgccgccccg ggcactgcgc
     5821 agcgcccccc agcggcaggt cagcgtcagc tggccgaaca gatccgttcc gatgctggct
     5881 tcataccagc gatcccgact ttcccagcgt ctgtagcagg attgcatggc cggctccggg
     5941 ggctgtcgcc gaggaaagac atggattgtc catagaaatc atcagcatgg aagaatgcag
     6001 gcattcatgt cgccatggag cgacagtttt ggacagaagc ctgcgtgcat tgcagcatta
     6061 aatatcggca agtatatgat ccatacagaa aagctttgga tggcacgaat cctgtcaaag
     6121 ttcccggcag gccggtcgat ccggccgagc agccaggccg gtgtggccgg ccgtcatttg
     6181 cgggagttcc gacatgaaag ccaccgatct gatcgatgac ctgaaccgcc ggttcgccga
     6241 cgatgactat ggccggcggg cgccggcggc cctgcagcgc agcagcgacg gcgcagttgg
     6301 cagcttccag ggactgcgcc tgtcgaccgg cgttgaaacc ctgttcgcgg ccgatggcgg
     6361 catcagcggc catcaggccg tgaccgtcgt gcgcggcccg catggccggc cgctgtcggc
     6421 gtgggtgccg tatgcgctgg cgctggatgc acaggcgatc ggctggctgg atcggctggt
     6481 gcgtacgctg catgtgttca acgcccgtgc ggtgctgtcg ctcgggcggc tgtggctggc
     6541 cgtgcatccg ctgcatctcg gggcgatcag cggcgagttc ggggccgtgt tcgcgaatct
     6601 gctggcacgc atgcgcgtgc atccctcgca gatcgtgctg gagatcgccg acgccgatcg
     6661 catgccgcgc tcgcggctga tgcaggcctt gtccggcttc cggacccacg gtttcggcac
     6721 ggcctgcgtg ctgcgcggcg atgatccggg cgcgttcgag caggtgctgt ccctgcatcc
     6781 ggacgccgtc aagctctgcc gcagccatct gtatgcggcc cgcgcgtcgc tgctcggacg
     6841 cgagcgcctg gcacggcgga tcgcagcgat ccgggcggcc ggtgcgcatg cctggctgcg
     6901 cggtgtcgac gatctgccct cgacgcgact ggcccgcgcg ctcgatgccg acggctggca
     6961 gagcggtctg ctcgacgccc gcatgctcgg cggccacggc ggctgaacga acaccctgtt
     7021 tcgtgatcgg accgcgcgct ttcagcggcc gcttgcgctc aggcgctcga tgaggcgggc
     7081 cgcggccctg agcccgaaac tcgccgtcat gcacatcgat gcgccataac cggcgcagtt
     7141 gagtccggca tcgccggcac aggagcgcgg tcgcagcacc ggctccggcg accagacgca
     7201 gtcgatgccg aagcgccgcg ccggatcgcg tggaaagccg tactccttgc gcaggcgctg
     7261 gcgcagcttc gccagcatcg ggtcctgctc ggtctgcgcg aggtccgaca gccggatccg
     7321 ccccgggtcc tgcttgccgc cggccccgcc ggatacgacc agcggcagat gcgcgctccg
     7381 gcagcaggcg atcagcgcgg ccttggcgcg gacgttgtcg atcgcatcga ggacggcatc
     7441 ggcaccggcg agcaggccgg cggcgttctc gggggtcagg aaatcctcga cggcttccac
     7501 cgtgcaggcc ggattgatcc ggccgatgcg ttcggcgagc acctcgacct tagcccttcc
     7561 gaattccccg tcgagtgccg gcagctgccg gttgacgttg gactcatgga cgatgtcgag
     7621 atcgatgatc cgcagcgtgc cgatcgcact gcgcgccagt gcctcgacgg cccagctgcc
     7681 gaccccgccg acgccgacga ccacgacgcg cgccgcctgc aggcgggcga gagcgtcgct
     7741 gccatacagc cgggcgatgc cgccgaaacg gcgttcagga tcagaggacg ggatcgacgg
     7801 gatcagcggc agcggggaag cggacacgaa cgtgcagacc gggcggcagg ggctggcacg
     7861 ataggcgagg cagggagatg cgtcgagcgg cggccgtggg cggctgcagt gccgggtgcc
     7921 ggctcagaga tcgaagcggc tcacgtcgat gccgagcgcg cgggccagcg gtacgagtgc
     7981 cgagcgccgc ggcggcgtca gcgggttttc ctcccaggcc tggatcacgc gcgccgatac
     8041 gcccatgcgc tcggcgacgg tctcgcggct gatgccgaga tattcgcgcc aggcccgcag
     8101 cggagtatct ccgtacacga cgacctgcgt gatcaccgca tgaggcacat ggacgctgtc
     8161 atagccatgc ccctgcgggc cgggcatggc gtgcagtgcg tgcatggaca tggcggtctg
     8221 ctccggattc agggctgcgg acatgccgcg catcatgagc gctgcggcgc cggaaacgac
     8281 tcgctgcacg gggactgaca cagcgttttg caggatttcc gttcctgccg tcgtccggga
     8341 catgcgtgtg accccgttac cgggcggcgg ttcagctgcg gcaatccctg cctgccgcgc
     8401 aggcggcaag tcgtggaaat ttgcatacga cctcgtccgg aagcactgca ttcgctgaaa
     8461 gctgcgatac actcggccca ttacccgatc aggaccgatc gatcgaaatg cctgcacctg
     8521 acgctgcggc atcgttcgag gcatcactcg tccggattcc ggtcagctgc accagttcag
     8581 ggcgtcatga cgcctcgacg cgcacgcgtc gtcgaatcga agatcatcat tccgatctgc
     8641 ccggtgaacg ggcggttttc tgccgttgtc caaacggtat tactgcaccc cactccggag
     8701 ggagtacacg gcgccatgcc gatcagagcc gacctttccc atatcgatct tgccgcgatg
     8761 tcactgccgg atttgcaccg actcgtggat aaaattgaaa aagccataga aatcaagctc
     8821 ttcgagcgag gcgcccagcg cgctctggcc gtgttttccg cgcatgcccc gacccctgcg
     8881 gcggctccgc ccgtgggtcg cggcggtaac cgcgacgtgc gcaggaccgg tcgcgagcgc
     8941 agcggacggg attccggact gcgccggcgc tgacggacac ccccgcattc gacggccata
     9001 gccgacgctg attggccggc ggttctgtta ttccgaatat cacgcgggac ggcattcctc
     9061 actgctgacc gggctggcgt tttccgtggc accggctcat tcggctgcgt gcaatccaca
     9121 atccggccat ggtcacgacc gaccgctgcc tgcccggcgc tcattgcgct gccggtgtgc
     9181 cggtcgatcc gtcttcggca gcccgccaca gataccaggc gagcacgctg cgccacggcc
     9241 gccaggcttc cccccagtgc agcagttccc gggcccgcgg caatccggga ttggcctcga
     9301 tcacgcacca gccccggcgc accccgtaat cgtcgaccgg ccagacgtcg ggccgtccga
     9361 gcgtgaagat caggaacatc tcgacggtcc agcgcccgac gccgcgcgcg gccgtgagcc
     9421 ggctgacgag ttcatcgtcc gtcagcctgt ctgcttcggt ccgatcgggc agaaagccgc
     9481 tcgcggcacg tgcggcgatg tcccggatcg cagccgcctt ctggcgcgac aggccggtgc
     9541 cgcgcagatc gtcatcgctc agggccagca ccccgtccgg aaccggtgcc gtactgatcc
     9601 ggctgcggaa acgcgcctcg atcgcggccg cggcgcgccc ggcgagctgc tgatgaatga
     9661 cggccgagca gagggccagc cagggctgca cgccgggcag cggggtgatc gggcaggggc
     9721 cgtgacggct gatcagctcc gccagccccg gatggcggga cagctgccgt tctgcctgtt
     9781 gaacgcctgc aatcgtcagc aactcgggca tggcaagagg tacgggcccg gcagggattc
     9841 gagatcgcgc cgccctgcac cacggccatg agcaggatcg gcaagcgtca gggcatgttt
     9901 caggccgccc gcagtctcgc gatcggccgc cagactcgtt taaggtgcag cgccggtcag
     9961 cggctcggaa agaccgaccc gaccccggcc gtgctggagg atacagatgt tgcaccgatt
    10021 tcgccgcggc ctgtctgccg ctgccgtcgc catcaccctg gcgggcaccg gcaccggtgt
    10081 cgccggcacg agcgccgatc cggaatatct gcagcgtgcc gaggagcatc gcaaggcgca
    10141 tgacctgccg gccgccatca tcgagctgaa gaacgctctg gccgaggcgc ccggggatgc
    10201 ccgcgtgcgg ctgctgctcg gcagcctgta tctggaacag ggcgacggag ctgccgccga
    10261 gaaggaactg cggctggcgc gcaggctcgg cgtgccgctg ccgcggctga tggcagggct
    10321 ggccgaagcc atctatctgc agcgggcctg gcagcgactg gtcgatgacg tgcagccgct
    10381 gaccggcatg agcgcccgcg aggtggccga cgtgcgcgcg ctgcgcggcg aggcgcagtt
    10441 cgcgctgcgg cgcaacgacg aagcccgcac ctcgctggaa agcgcactgc gcaccgatcc
    10501 ggccgccgtg cgggcgctgc tcggcagtgc ccggctggcc ctctccgaac agcgccacga
    10561 tcaggcccgc gtctggctgc agcgggccgt cgaccagtca ttgagtccgc agcttgccaa
    10621 cgatgcctgg tcgcagttcg gcgatctcga ggttgccacc cggcatctgc ctgcagccga
    10681 tgcggcctac accagagcca tcgacaccgg gcagagtcat ctcggcgtcg tgctgcgccg
    10741 cggactggtg cgggtggagc tgcaggacta tgacggtgcc gccgccgatc tgaaccgcgt
    10801 cgccggccgg gcgcccgacc acacgctggt ccattacggc ttcgggctga tggcctaccg
    10861 gcaggggcac tacgccgagg cggccgcttc cttcagccgt gcgcggcagc gggcgccgga
    10921 caacgccgat gccagctact acctggccgc ctcgcagctg gcgctcggac agctcgaccc
    10981 ggcgatcgac ggcctgtcgc gcttcctggc cgagcatccc gaatccgagg tgaccgcccg
    11041 cctgctcggg aatgcctatc tgcgccgcgg ggatgccaac gccgctgccg cgctcatcca
    11101 gccgctgctc gaacgccagc ccgatgatgc cgcgctgctc gatctgctcg gccgcatcac
    11161 gctcatccag ggcgatgccg cgcgcagccg cgaactgctg gcgcgtgcca ccgaactggc
    11221 accgtcttcc gccttcggcc gctatgccta cggcatgagc ctgctcgccg ccggcgacca
    11281 gaaccgcggg ctggaggagc tgcagaaagc cgtcgagctc gatccgcacg agcacggcct
    11341 ggagttcggc ctgagcgtca gcctgctgcg cgccgggcac ttcgacgagg ccatcgccgt
    11401 cgcgcgccgc ctgcagcaga gcctgccgga ccaggccgag ccgctggtgc tcgaaggcat
    11461 tgcccaggcc gcccgccagc aggatgccga agcccgtgcc gccttccgcc gggcgctggc
    11521 actggagccc ggacatccgc gggccgccgg cgccctcgcc gccctggacc tcggcagcgg
    11581 agacatcgat ggcgcgcgcg gctggtaccg gcagatcctc gcccatcacc ccgggcatgc
    11641 cccgacgctg atgcagctcg cccggctcga agcccggcag ggccagggcg aggcctatgc
    11701 cgaaaccctg cgcgaagcca tcgccgccga tccgcacgtg ccgcagccct acgtgctgct
    11761 cgcccgctgg cagctgcgtc agggtcagcc cctgctcgcc gtcggcaccc tggtcgcggt
    11821 acgcgccgag tttccggagt atccgccgct gctgctgacg ctcggcctgg cacagggcgc
    11881 aaacggcgaa agcgccgatg cgatcggcac gctgcgccgg ctgatcgaac tgcagccgca
    11941 gtcggccgag gcccgctacg cgctcgtgac cgcgcacgcg caggccggcg atgccgcggg
    12001 cctgcgcggc acgctcgcgc aagccctggc cgtcgacaac cgccgccccg aagccctgcc
    12061 cctgctcgaa cgcctggtcg gtctcgccgg cagcgccagc gaggccgagg actggctcga
    12121 agcgctgctg cgcgatcgcc cggacgacga gatcgtgctc gacttcatcg gccagcgccg
    12181 ctaccggcaa tacggcgccc cgaccgcgct gacctactac gcccagctgc agcgccgcca
    12241 gccgcaggcc gcgctgtggg tgctgcgcga ggccgagctc cgcagcggca accgcgaggc
    12301 cgcgcaggcc caggccctgc tcgaacgctg gctgcaggca cacccggatg acgtgcaggc
    12361 gcgcttcgcg ctcggcctga tccacctcga cgccgatcgc cgggccccgg cccgcgccgc
    12421 cttcgaggcg gtgctcgaac gccggcccga ccagctcgcg gccctgaaca acctggcctg
    12481 gctgctgcgc gaggacgatc cgcagcgggc gctggactac gcgaaccgct gcctgcagct
    12541 gcgcccggcc gatccgcggc tgatggacac gctcggcgag gtgctgatcg cgcagcacca
    12601 gtacgcccgg gccgccgagg tgctggcgaa ggccgtcgcg cgcgccccgc agctgggctc
    12661 ggcccgctat cacctggcac ttgcgctgtc gctcgacgga cgcagggacg aggcccggga
    12721 gctgctgaaa tccctgctcg ggaatgatca gcccttctcc gagcgcgccg aggcgcagag
    12781 cctgctcgcg cagatcggca gctgaatccg ctccggttca ccgggtcgcc ggcaagggcc
    12841 agggggccgg ccggcggccc gccgggtttc atcggcttca tcgatcgagg gacttgcatg
    12901 atgtggaaca cccggatgcg gccgcttgcg gctgcgctgc tgctgctcgc cggcagcgca
    12961 accgccgcag ccgacctggc cagggaactg gacaaggccg aacagctgcg cgcgcagggc
    13021 cagctcggcg ccgcgatcat cgaactgaag aacgcgctgc aggacgagcc cgattacggg
    13081 ccgggccggc ggctgctcgg cgtgctgtac gtcgagctcg gcaacggtgc ggaggcggaa
    13141 aaggaactgc tgcgcgcccg gcggctgaag gtccgcgaga ccggcatcgc actgccgctc
    13201 gccgaagccc tgctgctgca gcgcgaatac cagcgcctgc tcgaaagcgt gcagccggtc
    13261 tcgggcatgt cgatgcgtga tgtcgccgac ctgcgcgcgc tgcgcggcga ggcgctgctc
    13321 gcgctcggcc gccagggcga cgccgaggcg gccgtgaagt ccgcgctcgc caccgacccg
    13381 gccgcgccgc gtgcgcagat cgccgaggcg cgtctcgcac tcgcggcccg ccaccccgac
    13441 accgcccgcc agcgcctgca gaccgtgctg tcggccccgc tgccgcagcc ggtcgagcac
    13501 atcgcgcagg cgctgctcgg cgatgtcgag atcatggccc ggcgcctgcc ggaggccgcg
    13561 aaggcttacg accgcgccat tgccaccggc cggccgcacc aggcgctggc gatccagctc
    13621 aagcgcgggc tgctgcgcat cgatctcggt gatcccgacg gtgcccgtgc cgatctgggt
    13681 gcggtcaacg cccggctgcc ggatctgccg ctgccgcatt tcggcttcgg gctgctcgcg
    13741 taccggcagg ggcgctatgc cgatgcgctg gattccttcg aacgcgcgct gcagcgcgat
    13801 cacgagaatc ccgacatcca cgccaagatc gccgccacgc gcatcgcgct cggccagcgc
    13861 gagcaggcgg tcaccgatct ggaaaaattc ctgcaggaac atccggacag cgatccgatc
    13921 ctgcgcctgc tcgcgacgat tcgcctgcag cagggcgata ccgccggcgc gcagaaactg
    13981 ctgcgccacg tgctcgaact gcatcccgac gacaacgccg tgctcgacat gctcggcgcg
    14041 atcgcgctcg cccgcggcga agcgcgcgag ggcgagccgc tgctgcgccg ctcggtcgaa
    14101 cgcgatccgg attcggccgc ggcgcgctac cggctcggcc tcggcctgct ggcactcggc
    14161 gatcgcgaga acggcctgcg cgaactcgcg caggccaccc agctgcagcc cggtcaggaa
    14221 cgctacgaat acgcactggc gctcgggctg ctgcgcagcg gccagcatgc cgaagccgtc
    14281 gctgccgccc agcgcctgca ggcgctgtgg ccggatcgca acgaaccgct gctgctcgaa
    14341 ggcatcgccc gcgcgagcgc cctcgacacc gagggcgcca gtgccgcctt cgagcgcgtg
    14401 ctcgcgcgca gccccggcga cccggccgca tcgggcggac tggcgacgct cgacctgcag
    14461 cagggccggc ccgacaaggc ccgctcgcgc tatcgggccg tgctcgaaca gcatccgggc
    14521 catggggcca cgctgctgga gctggcagcg ctcgaactgc ggctcgacaa ccccgaggcc
    14581 gcccggcgcg cgctggagca ggcggtcgcg gccgatccgg aggcgctgga gccgcgcctg
    14641 cgcctcgcgc gcctgctcgg gcgcgagggc cggcatgcgg cggcgctcga actgctcgaa
    14701 gccgtgcatg cccgccatgc ggacgatgtg cagctgctcg cgctgctcgg agccgcgaag
    14761 accgcactcg gggatgccgg cggcggccgc gccgcctacc gccgtctgct cgatctcgat
    14821 ccgaagtccg ccaccggcct gtacggctac gcctcggcct gcgccgccgg cagcgacgac
    14881 gccggcctgc gcgcggcgct gcgtccggcg ctcgccgccg accccggcaa tgccctcgcg
    14941 caagcgctgc tggcacggct gcaggaactg gccccgggcg aggccgccgc ccgcgcctgg
    15001 ttcgaggaac tgaaccaggc gctgcccgac aacctcgcgc tgctcgaatt cgagggccgg
    15061 cgcatcgaac gccagcaggg caggcagccg gcggcgatct attacggcgg cctgcgccgc
    15121 cggcagccgc acgaacagct ctgggcgctg cgcgaggtcg aactctggca gcaggcgaag
    15181 gagccggccc aggcgcgctc ggtgctggtc gactggctgc gctggcatcc ggacgacgac
    15241 acggtgcgcg cccagctggc ggcgatgatg ctcgccgccg gccagaacgc cgatgccaag
    15301 gccgagctgg agcgcatcgt cgccgacaac ccgacgcgcg tctcggcgct gaacaacctc
    15361 gcctggctgc tgcgcaagga cgacccgcgc cgtgccctgc gtctggccga gcgcgccgtg
    15421 cgcctgcgcc cggagccgcg catcctcgaa accctcggcg aggtgctgat tgccggcggc
    15481 gaatacgtgc gcgcgaccga ggtcctgcag tacgccgccc tgcgcgcacc gcagcagccc
    15541 tcgatccgct accagctcgc cgtggcgctg gccgggcagc agcgcaagga ggacgcccgc
    15601 cggctgctga aggaactgct cgccggcgac acccgcttcg ccgagcgcag cgacgccgcg
    15661 gcgctgctga agaagctcgg cggctgacgc aggcgttatc ccgggaccgg ggctgccggc
    15721 tgcggatatg ctgcggccct ccgttcatca ggatggtgat ccatgccccg aaccgccctg
    15781 taccgcacgc gcgccgccgt ctgcgcctgc gtgctgatcc tgtccgggac cggcgcgcag
    15841 gcggacgatg atgtcctgac cgctgccggc cgcaagctcg aagccggtca ggtcagcgcc
    15901 gccgtcatcg acatcaagaa ccgcctgcag cagcgccccg gtgacggggc tgcccgccgc
    15961 ctgctcggca tcgcctacgt gcggcttggc gacggtgcga acgccgaaca ggaactgatg
    16021 cgtgccgcgc agctcggcgt gccggccggc gagctggccg tgccgctggc cgaagccctg
    16081 gccgtgcagc agcaatggca ggcgctgctc gatcgggcga tcgtgcagcc gaacatgcgc
    16141 acgccggagc tcgccgagct gcgggcgttg cgcgccaccg cccatcaggc cctcggccag
    16201 accgggcagg ccaggctgca gttcgaggcg gcggtggcga cggatgccgc ttcggcacgc
    16261 gcgcagctcg ggctcgcgca gttcgatctg caggccggcc gctggccggc cgcgcgcacc
    16321 cgactccaga c
//
LOCUS       DAOQIE010000040        16273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1676_length_16273_cov_7.492231, whole genome shotgun sequence.
ACCESSION   DAOQIE010000040 DAOQIE010000000
VERSION     DAOQIE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_16273_cov_7.492231"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PK144_04165"
     CDS             <1..1825
                     /locus_tag="PK144_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNF64954.1"
                     /translation="QRCREAFAEVPVVIGGIEASLRRIAHYDYWSDKVRRSVLLDAKA
                     DLLVYGNAERAIVEIAHRAAAGESIRTMRDIRGTAFMHAGPLPEGWAELDSSQLDTPG
                     RIEPLPNPYEETPPAAACATPPADASGAQVVRFERRPRHPRERTVVRLPSYEQVRDDP
                     VLYAHASRTFHLETNPGNARALVQRHGEREVWINPPPLPLATPEMDSVFGLPYARVPH
                     PAYGGARIPAYEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSEASILAEIEDIRD
                     KTPGFTGVISDLGGPTANMYRLACRSADIERSCRKLSCVWPDICPNLNTDHSALVGLY
                     RRARALPGIKKILIASGLRYDLAVRDPEYVQELVTHHVGGYLKIAPEHTEAGPLSKMM
                     KPGIGSYDRFKEMFERFSKAAGKEQYLIPYFIAAHPGTRDEDMMELALWLKRNGFRAD
                     QVQAFLPTPMALASAMYHSGYNPLKGVRRAGQAERVESPKGLKQRRLHKAFLRYHDPD
                     NWPLLREALKRMGRADLIGNGKAHLVPGFQPAGTGLGGEGARTGRKHGLQRLLTQHTG
                     LPPRADGSRPARGPAPDPQAAGGRAKEKAAQGAARRGDRRR"
     gene            complement(1834..1986)
                     /locus_tag="PK144_04170"
     CDS             complement(1834..1986)
                     /locus_tag="PK144_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64955.1"
                     /translation="MIRFRPDTKQLCVLALAAALALVSLMVPIDDGKPGPGSEAPAGE
                     HLAHGG"
     gene            2047..2865
                     /gene="mazG"
                     /locus_tag="PK144_04175"
     CDS             2047..2865
                     /gene="mazG"
                     /locus_tag="PK144_04175"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNF64956.1"
                     /translation="MSDVARLLQIMSLLRDPERGCPWDVAQTFASIAPYTLEEAYEVA
                     DAIEREDFAGLKSELGDLLLQVVFHAQMASEQGRFGFAEVVATLSDKLVRRHPHVFGD
                     TTYASLEEQGEAWERIKREERAADGEIPAGALDGVPLALPGLTRAVKLTKKAARVGFD
                     WDEPGAVLDKIEEEIRELRAEIHTGAPLARLRDELGDVLFALANLARHLDLDPEAALR
                     GTNAKFERRFRHIEARLAETARTPADATLAEMDGYWNEAKALERGEAPEPSTTR"
     gene            complement(2871..3350)
                     /locus_tag="PK144_04180"
     CDS             complement(2871..3350)
                     /locus_tag="PK144_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNF64957.1"
                     /translation="MPCPPRPGNSAAAAIIRACLALAVPWLAAAPATAGQTTQVTVGA
                     GRFRVEVAETEAERERGLMYRAALPAGSGMLFVQPRALPVSFWMKNTHVALDILWFDA
                     QGRLVKISARVPPCTADPCPLYPSGGPVAWVLEIAAGEAARQRIRIGDRLKVEAAKD"
     gene            3349..3954
                     /locus_tag="PK144_04185"
     CDS             3349..3954
                     /locus_tag="PK144_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF64958.1"
                     /translation="MEYRYRIIDRAIAYQGFFTLSEYRLQHERFAGGWSDEISRLCFE
                     RGDAVAVLLWDPRRDAVVLVEQFRVGALGRPYSPWLYEIVAGITGPGETREEVARREA
                     VEEAGCTVEALAFVCEYLCSPGGTSESTALYVGCVDSHGVGGIHGLAHEHEDIRVFVV
                     PRAEAMAMIGGRLNSAAVIIALQWLALNRERLVAEWLPAGG"
     gene            complement(3985..5805)
                     /gene="typA"
                     /locus_tag="PK144_04190"
     CDS             complement(3985..5805)
                     /gene="typA"
                     /locus_tag="PK144_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNF64959.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTLGERAQATERMMD
                     SNDLEKERGITILAKNTAIRWNDYRINIVDTPGHADFGGEVERVLSMVDSVLLLVDAV
                     DGPMPQTRFVTQKAFAMGFRPIVVINKIDRPGARPGWVLDQTFDLFDRLGATDEQLDF
                     PVIYASALNGYASLDPECRDGDMTALFEAIIEHVSPPAVDLEGPFQMQVSSLDYNSYV
                     GVIGIGRIKRGSVRTNTAVTVVDAEGNRRQGRVLQILGFHGLERVEFPEASAGDIIAF
                     TGMEKLNISDTLCDPAAVEALTPLSVDEPTVSMTFQVNNSPFAGKEGKFVTSRQIRER
                     LQRELIHNVALRVEDTEDPDKFKVSGRGELHLGILIENMRREGYELGVSRPEVIIREL
                     DGEKQEPYETLTVDVEEQHQGRMMEKLGERGGELRDMVPDGKGRVRLDYLIPARGLIG
                     FQTEFLTTTSGTGLMYHVFDHYGPYKRGEFGARINGVLIANAAGKALAYALFNLQERG
                     RLFIGHGEEVYEGMIIGIHSRDNDLVVNPLKAKQLTNIRAAGSDENIILTPPIRMTLE
                     QALEFIDDDELVEVTPKTIRIRKKLLDENARKRASRKSEE"
     gene            complement(6003..6209)
                     /locus_tag="PK144_04195"
     CDS             complement(6003..6209)
                     /locus_tag="PK144_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNF64960.1"
                     /translation="MRTGTIKWFDEAKGYGFIQPEDGTGDVFVHYSVIDGKGFRTLAE
                     GQKVEFDSRQGNKGPQATVVKPLA"
     gene            complement(6224..6427)
                     /locus_tag="PK144_04200"
     CDS             complement(6224..6427)
                     /locus_tag="PK144_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005287157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNF64961.1"
                     /translation="MKTGTVKWFDEAKGFGFIAPSDGTGDVFVHYSAIEGKGFRTLAE
                     GQKVEFESKEGQKGPQATSVKAV"
     gene            6692..10810
                     /gene="hrpA"
                     /locus_tag="PK144_04205"
     CDS             6692..10810
                     /gene="hrpA"
                     /locus_tag="PK144_04205"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNF64962.1"
                     /translation="MLHGRLPPAPPPAVPDPLRALAGLLPQCLAVDREDFRRRLDRLE
                     RRRDDGRAHERGLAELITAMEASHARLLERRARLPLPVFDDALPVNQRREDIAALIRA
                     HQVVVVCGETGSGKTTQLPKICLELGFGAAGLIGHTQPRRIAARSVAARIADELGAGA
                     AAAVGYKVRFSDRTGPQAAIKLMTDGILLAETQGDPLLSAYEVLIVDEAHERSLNIDF
                     LLGYLKRLLPRRPDLKLVITSATIDPQRFARHFDDAPVIEVSGRTYPVEQRWRPLIGE
                     DEDARDRDLQQAILDAVDEVWAEGPGDVLVFLSGEREIRETAEALRKHHPPGVEIVPL
                     YARLSAAEQNRVFEAHGRSRIVLATNVAETSLTVPGIRYVIDPGTARVSRYSYRAKIQ
                     RLPVEKISQASARQRAGRCGRVEAGVCLRLYSAEDFAARPAFTDPEILRTNLAAVILQ
                     MAALNLGDPAEFPFVEPPDSRLIHDGYKLLHELQAVDERHRLTALGRELAKLPIDPRL
                     GRMILAARDEGCLAEVLVIAAGLAIQDPRERPLEVQQAADQQHRRFRDERSDFLALLR
                     LWDWWQAQGRRLSKSKLRELAKASFLSYVRMREWRELHGQLVEVLATLGAVQNEREPA
                     PPPPEPNRKREDGDSLWPANEVPIHRALLAGLLGHTGLRQQEGSEFLGARGRRFRIWP
                     GSGLTKKPPRWLMAAELVETTQVWARTCARIQPEWLETLAAHLIKRAYSEPHWEKRAA
                     QVAATERVTLYGLPVVTGRKVNYGPIDPPLARELFLRHALVNGEFDCRAPFVAHNAAL
                     IAEIARIEDKARRRDVLVDEEAIFRFYDARVPERVWSGAAFLKWLKAAGREDPQLLFL
                     SRETLMAHAAEQVTDARFPDRLVLAGIEFPLAYRFEPGAADDGVTLTVPLAALNQVPA
                     ARCEWLVPGLLPERLAALLKTLPKELRRRVVPVPDTVEKLLPELDAHADAEAPLLPAL
                     VAALRRLTASEFPEDAFDPAAIPAHLGMNFRVVAPDGAVLAEGRDLRRLRAQFGDAAR
                     EAFGTLASAFRAAADKARTPLRARGEPVIPASPAPAPKPAMPAEDPVARLERAEVHDW
                     DFGALAQTIEFVRNGIVLKGWPALVVEGERLAVRVLDTEARAAAAHRHGLHRLISLRL
                     REPVKYLRRNLPGLAQMGLRYVAIGTQEQLRDDLLAAAIDRAFLVPALAGGGLPRDRA
                     GFEAMLEHGRAQFVGCANEVCAQVGQALASFHAVRRRISGQVPLAWMEAAKDMHEQLD
                     ALVYPGFVSATPPEWLRRLPVYLKALELRVERLGGRLEQDRQRSAEVQRLWRWYLQRR
                     TQLARRGADDPELERYRWMLEELRVSLFAQELKTLFPVSVKRIEEQGAKVRV"
     gene            complement(10821..11924)
                     /locus_tag="PK144_04210"
     CDS             complement(10821..11924)
                     /locus_tag="PK144_04210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNF64963.1"
                     /translation="MSRPPLLTRIPDAIAAVPDYATYARERLDPAIWAYLDGAAGDEL
                     SARWNREAWERLRLHGRVLADVAGGDTALTLFGSRLEHPVLLAPVAYQRLAHPDGELA
                     AARAAAALGAGFVLSTLASVELEAVAALPDAPPLWFQLYCQPERAASVELVQRAEAAG
                     YSALVVTVDAPLAGIRNRERRAGFRLPPGVSAANLRHGQTPFAPGSAQASLVFDGIMR
                     TAPTWRELDWLLDQTRLPVILKGIVSPEDAALAIERGVAGLIVSNHGGRVLDTLPASA
                     EALPVIAERVAGRVPILVDGGIRRGTDVLKALALGASAVLIGRPYLHGLAAAGALGVA
                     HVLKMLREELEVAMALTGCARLADIGPGVLWRP"
     gene            complement(12215..13141)
                     /locus_tag="PK144_04215"
     CDS             complement(12215..13141)
                     /locus_tag="PK144_04215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF64964.1"
                     /translation="MSSPEMPHILVIDDDPFNHVLLAEGLGDEFELLFAKHWREVVEI
                     VGHSPRLDLVILDVDMPEVDGFEVCRRLKDTEFGRELPLIFMTGVDDVESQIKGFSLG
                     AVDYIMRPFALPVVRARLRTHVTLRRQTQLLQQLALMDGLTGIANRRCFDQHYHREWR
                     RALRTGCPLGLALIDADHFKLFNDRCGHGAGDDCLRRIAALGREAMRRPGDLIARVGG
                     EEFALLLPETEAAGVHCVVDKLREGIAARLQGQYRATDDGLRLPVTVSIGCGAIRPDR
                     LDDPADFLTRIDRRLYRAKSGGRNRVVSDDGA"
     gene            complement(13145..15127)
                     /locus_tag="PK144_04220"
     CDS             complement(13145..15127)
                     /locus_tag="PK144_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64965.1"
                     /translation="MNELLPLPDDPETLQAEVLRLRKVVTALIRRAEKSTTSLESAES
                     GFTLYHTAALLEDHVRERTLELEATLAENERVTHDLRLAKQALEAEIAERRRNEAGLR
                     QAAMVYSSTQDGIVVCDPEGTVVAVNPAFSNITGYAEGEVLGRNMRMLQSGYEGRAFY
                     LDFWRALQQSGHWQGEIRNRRRSGEPYLQWLTINAVRDADQTIIRYVGVFSDITAVRN
                     AHDLEHLAHHDALTGLPNRLLLIAELDQALRRAARSGGGLAVLFLDLDRFKLVNDSFG
                     HTAGDELLVRAAGRLRERLRQSDLLARLGGDEFVVVLEEVTEPERAAGVAQTLIEALQ
                     QPFLLGGGHEVWIGASVGISLYPADGAEAGALIQRADTALYRAKEDGRGNARFYLEAM
                     TRAVEERLVLEARLRRALERGELVLYYQPQIMLGEPAALGVEALVRWRRPDGKMVPPS
                     DFIPLAEETGLIVPLGEWVLREACRCLRRWLADGVPIHSMAVNLSARQLRLHALAPQL
                     AAILDETGVPGRYLELELTESALMENPDTVAQQLEALRRLGVSLAIDDFGTGYSSLAY
                     LKRFPINTLKIDRSFVLDIPHDPADMQITAAVIALAHNLGLSVLAEGVETSEQLDFLI
                     EHGCDAVQGYRFSPPLPEAAACDWLCRHATTPAAGR"
     gene            complement(15120..16262)
                     /locus_tag="PK144_04225"
     CDS             complement(15120..16262)
                     /locus_tag="PK144_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNF64966.1"
                     /translation="MESASARILTGHTQACEVGLAVRELHAQISQPDAALVVVFCSTA
                     YPRPVLAEALQHHFGDVPMIGCTTAGELGASGYQTGGITGFSLPAAEFTVATARLDGL
                     DDVSLDAGQHCVDALLAQMPAVTDGQERFALLLVDGLAQREEPVAYALQRALGRTPLV
                     GGSAGDGLHFGRSWVYHDGAFHTRSAVLALVSTRRRSRVFKTQHFTHDKERLVVTEAD
                     PKRRRVFELNGLPAAQEYARLIGVPVAELAPETFAAAPVVVVIDGTDYVRSIREAHAD
                     GSLTFFCAIELGLVLRVAQARDMVAHLDSSMDSIRAGIGEPDLVISFDCVLRRLEARR
                     KHCEAALGERFRRYRMVGFSTYGEQYAGVHVNHTLTGVAIGRSGDE"
BASE COUNT         2434 a         5587 c         5779 g         2473 t
ORIGIN      
        1 acagcgctgc cgcgaggcct tcgccgaggt gccggtcgtg atcggcggca tcgaggcgag
       61 cctgcggcgc atcgcccact acgactactg gtccgacaag gtgcgccgct cggtgctgct
      121 cgacgccaag gccgatctgc tggtctacgg caacgccgag cgcgcgatcg tcgagatcgc
      181 gcaccgtgcc gccgccggcg aatcgatccg cacgatgcgc gacatccgcg gcaccgcgtt
      241 catgcacgcc gggccgctgc cggagggctg ggccgagctc gattcaagcc agctcgacac
      301 gccgggccgc atcgagccgc tgccgaatcc ctacgaggaa accccgccgg ccgcggcctg
      361 cgccacgcca ccggcggatg ccagcggcgc gcaggtcgtg cgcttcgagc gccggcccag
      421 acatccgcgc gagcgcaccg tcgtgcgcct gccgtcctac gaacaggtgc gcgacgatcc
      481 ggtgctgtac gcgcatgcct cgcgcacctt ccacctcgaa accaaccccg gcaacgcccg
      541 cgcgctggtg cagcgccacg gcgagcgcga ggtctggatc aatccaccgc cgctgccgct
      601 cgccacgccg gagatggatt cggtgttcgg cctgccgtac gcgcgcgtgc cgcacccggc
      661 ctatggcggt gcgcgcattc cggcctacga gatgatccgc ttctcggtca acatcatgcg
      721 cggctgcttc ggcggctgta cgttctgctc gatcaccgag cacgagggcc gcatcatcca
      781 gtcgcgctcg gaagcctcga tcctcgccga gatcgaggac atccgcgaca agacgccggg
      841 cttcaccggc gtgatctccg acctcggcgg cccgaccgcg aacatgtacc ggctggcctg
      901 ccgctcggcg gacatcgagc gcagctgccg caagctctcc tgcgtgtggc cggacatctg
      961 cccgaacctg aacaccgatc attcggcgct ggtcgggctg taccgccgcg cgcgcgcgct
     1021 gcccggcatc aagaagatcc tgatcgcctc gggcctgcgc tacgacctcg cggtgcgtga
     1081 tcccgaatac gtgcaggaac tcgtcacgca ccacgtcggc ggctacctga agatcgcgcc
     1141 cgaacacacc gaggccgggc cgctgtcgaa gatgatgaag cccggcatcg gcagctacga
     1201 ccgcttcaag gagatgttcg aacgcttctc gaaggcggcc ggcaaggagc agtacctgat
     1261 cccgtacttc atcgccgcgc accccggcac ccgcgacgag gacatgatgg aactcgcgct
     1321 ctggctcaag cgcaacggct tccgtgccga tcaggtacag gccttcctgc cgacgccgat
     1381 ggcgcttgcg agtgcgatgt atcactccgg ttacaacccg ctgaagggcg tgcgccgcgc
     1441 cggccaggcc gagcgtgtcg aatccccgaa gggcctgaag cagcgccggc tgcacaaggc
     1501 cttcctgcgc tatcacgatc cggacaactg gccgctgctg cgcgaggccc tgaagcgcat
     1561 gggccgcgcc gacctgatcg gcaacggcaa ggcgcatctg gtgccgggct tccagccggc
     1621 cggcaccggg ctcggcggcg agggcgcgcg caccggccgc aagcacggcc tgcagcgcct
     1681 gctgacccag cacaccggcc tgccgccgcg cgccgacggc agccgcccgg cacgcggacc
     1741 tgcgccggat ccgcaggctg cggggggacg ggcaaaagaa aaggccgcgc aaggcgcggc
     1801 ccggcggggc gatcgacggc gctgaggttg cgctcagccg ccgtgggcga ggtgctcgcc
     1861 agccggtgct tcgctgccgg ggccgggctt gccgtcatcg atcggcacca tcagcgatac
     1921 gagcgccagg gctgcagcca gggcgagtac gcagagctgc ttggtatcgg ggcggaatcg
     1981 gatcatggtt ggaagtctcc gcggacgggc gcgtccgcaa tctggaaatt ttcggcggat
     2041 actaaggtga gtgacgttgc ccggctgtta caaatcatgt ccctgctgcg tgatcccgaa
     2101 cgcggctgcc cgtgggacgt ggcgcagacc ttcgcgagca tcgcgccgta caccctcgaa
     2161 gaggcctacg aggtcgccga tgcgatcgag cgggaggact tcgccggact gaagtccgaa
     2221 ctgggagacc tgcttttgca ggtggtgttc catgcccaga tggcgagcga gcaggggcgc
     2281 ttcggcttcg ccgaagtggt cgcgaccctg tccgacaagc tcgtgcgccg ccacccgcac
     2341 gtgttcggcg ataccacgta cgcgagtctc gaagagcagg gcgaggcctg ggagcgcatc
     2401 aagcgcgagg agcgcgccgc cgatggcgaa atcccggccg gggcgctcga tggcgtgccg
     2461 ctggcgctgc cggggctgac ccgcgcggtc aagctcacga aaaaggccgc gcgcgtcggc
     2521 ttcgactggg acgagccggg ggccgtgctc gacaagatcg aggaggaaat ccgggaactg
     2581 cgggccgaga tccacaccgg tgcaccgctg gcgcggcttc gcgatgaact cggcgacgtg
     2641 ctgttcgcgc tcgcgaacct tgcgcgtcac ctcgatctcg acccggaggc ggcgctgcgc
     2701 ggcaccaacg ccaagttcga acgccgcttc cgccacatcg aggcacggct cgccgaaacc
     2761 gcgcgcaccc cggccgacgc gacgctcgcc gagatggacg gctactggaa cgaggccaag
     2821 gcactcgaac gcggtgaagc cccggagcca tcgaccacgc gctgaaggct tcagtccttc
     2881 gcggcttcga ccttcagccg gtcgccgatc cggatgcgct gacgcgccgc ctcgccggcc
     2941 gcgatctcca gcacccaggc caccggtccg ccactcgggt acagcgggca cgggtcggcc
     3001 gtgcatggtg gcacgcgcgc actgatcttc acgagccggc cctgcgcatc gaaccacagg
     3061 atgtcgagcg cgacgtgcgt gttcttcatc cagaagctca ccggcagcgc ccgcggctgc
     3121 acgaacagca tgccgctgcc ggccggcagc gcagcgcgat acatcagccc gcgctcgcgc
     3181 tcggcctcgg tttcggcgac ctcgacccgg aagcgtccgg ctccgaccgt gacctgcgtc
     3241 gtctgcccgg ccgttgccgg cgcagcggcg agccacggca cggcgagcgc gaggcaggcg
     3301 cgtatgatcg ccgctgcggc gctgttgccg ggtcggggag ggcagggcat ggaataccgg
     3361 taccggatca tcgatcgggc catcgcctat cagggcttct tcacgctgtc cgaataccgg
     3421 ctgcagcacg agcgcttcgc cggcggctgg agcgacgaga tcagccggct gtgcttcgag
     3481 cgcggcgatg cggtcgccgt gctgctgtgg gatccgcgcc gcgacgccgt cgtgctggtc
     3541 gagcagttcc gcgtcggtgc gctcggccgg ccgtattcgc cgtggctgta cgagatcgtc
     3601 gccggcatca ccggccccgg cgagacgcgc gaggaggtcg cccggcgcga ggcggtcgag
     3661 gaggccggct gcacggtcga ggcgctggcc ttcgtctgcg aatacctctg cagccccggc
     3721 ggcacttcgg agagcacggc gctgtacgtc ggctgcgtcg acagccacgg cgtcggcggc
     3781 atccacggtc ttgcgcacga gcacgaggac atccgcgtgt tcgtcgtgcc gcgcgccgag
     3841 gcgatggcga tgatcggcgg gcggctcaat tcggccgccg tgatcatcgc gctgcagtgg
     3901 ttggcgctga accgcgagcg gctcgtcgcc gagtggctgc cggccggcgg ctgagcgccg
     3961 gcgggccgtg tcaggtgccg ggtctcattc ctcgctcttg cgcgaggcgc gcttgcgggc
     4021 gttctcgtcg agcagcttct tgcggatgcg gatggtcttc ggcgtgacct cgaccagctc
     4081 gtcgtcgtcg atgaattcga gcgcctgttc gagcgtcatg cggatcggcg gcgtcaggat
     4141 gatgttctcg tccgagccgg ccgcgcggat gttggtgagc tgcttggcct tcagcggatt
     4201 gacgaccagg tcgttgtcgc gcgagtggat gccgatgatc atcccctcgt agacctcctc
     4261 gccgtggccg atgaacaggc gcccgcgctc ctgcaggttg aagagcgcgt aggcgagcgc
     4321 cttgccggcg gcattggcga tcagcacgcc gttgatgcgc gcgccgaact cgccgcgctt
     4381 gtacggtccg tagtgatcga acacgtggta catcaggccg gtgccgctgg tcgtggtcag
     4441 gaattcggtc tggaagccga tcaggccgcg cgccgggatc aggtagtcga ggcgcacgcg
     4501 ccccttgccg tccggcacca tgtcgcgcag ctcgccgccg cgctcgccga gcttctccat
     4561 catccggccc tgatgctgct cctcgacatc gacggtcagc gtctcgtacg gctcctgctt
     4621 ctcgccgtcg agctcgcgga tgatgacctc ggggcgcgag acgccgagtt cgtagccctc
     4681 gcggcgcatg ttctcgatca ggatgccgag gtgcagctcg ccgcggcccg aaaccttgaa
     4741 tttgtccggg tcctcggtgt cctcgacgcg cagcgcgacg ttgtggatca gctcgcgctg
     4801 caggcgctcg cggatctggc gcgaggtcac gaacttgcct tccttgcccg cgaacggcga
     4861 gttgttgacc tggaaggtca tgctgacggt cggctcgtcg acgctcagcg gcgtcagcgc
     4921 ctcgaccgcg gccggatcgc acagcgtgtc ggagatgttg agcttctcca tgccggtgaa
     4981 ggcgatgatg tcgccggccg aggcctccgg gaactcgacg cgctccagcc cgtggaagcc
     5041 gaggatctgc agcacgcggc cctgacggcg gttgccttcg gcatcgacga cggtgacggc
     5101 ggtgttggtg cgcacgctgc cgcgcttgat gcggccgatg ccgatgacgc cgacgtagct
     5161 gttgtagtcg agcgaggaca cctgcatctg gaacgggcct tcgaggtcga cggccggtgg
     5221 cgacacgtgt tcgatgatgg cctcgaacag cgctgtcatg tcgccgtcgc ggcactcggg
     5281 gtcgaggctc gcgtagccgt tcagcgccga ggcgtagatg accgggaagt cgagctgctc
     5341 gtcggtggcg ccgagccggt cgaagagatc gaaggtctgg tcgagcaccc agcccgggcg
     5401 tgcgccgggg cggtcgatct tgttgatgac gacgatcggc cggaagccca tcgcgaacgc
     5461 cttctgcgtc acgaagcgcg tctgcggcat cgggccgtcg acggcatcga cgagcagcag
     5521 caccgaatcg accatcgaca gcacgcgctc gacctcgccg ccgaagtcgg cgtggccggg
     5581 ggtgtcgacg atgttgatgc ggtagtcgtt ccagcggatc gcggtgttct tggccaggat
     5641 cgtgatgccg cgttcctttt cgagatcatt cgaatccatc atgcgttcgg tcgcctgcgc
     5701 gcgttcaccg agcgtgccgg actgctgcag gagcttgtcg accagcgtgg tcttgccatg
     5761 gtcgacatgg gcgatgatgg cgatgttgcg aagcttctcg atcacgacgg gaaccgggtg
     5821 gaggcagacg aaagtgccag tataaccgag ccatggccgc tgctgcgctg catcatgcga
     5881 agcggatgcg cgacaaccgg cggaggcacc ggaaagacgg cgagggcctg cgcgtggaaa
     5941 caaaaaagca aaccccgcca tcgaggcggg gttcgcagga ttcgtgttgt gctgcgggaa
     6001 gctcaggcga gcggcttgac caccgtggcc tgcggaccct tgttgccctg acgcgaatcg
     6061 aattcgacct tctggccttc ggcgagcgta cggaaaccct tgccgtcgat gaccgaatag
     6121 tgaacgaaca cgtcgccggt accgtcctcg ggctggatga atccgtaacc cttggcctcg
     6181 tcaaaccact tgatcgttcc cgtgcgcatc tccagctccc cgctcagacg gccttcaccg
     6241 aagtcgcctg aggacccttc tgtccttcct tcgattcgaa ctccaccttc tggccttcgg
     6301 ccagggtgcg gaaacccttg ccttcgatcg cagagtagtg gacgaacaca tcgcccgtgc
     6361 cgtccgacgg agcgatgaat ccgaagccct tggcctcatc gaaccactta accgtaccag
     6421 tcttcataga atgcgaaacc tcggaaaaac catctggcgc gccgccggca gtcgcgggtg
     6481 acgatgcatt ttcaatggga gatcgagaat ggatcgggac gggcggcctg ggcggcgcgg
     6541 tattcgatcc ggcgacgctt atcgtttgcg tcttgaggcc ggacccgagc tggacactac
     6601 gccaaaggct caaggcctgc aagccaattt cacgattcga cgatgaccat cccgcaaggt
     6661 tgccgtgcgc gtggcaagcg ccggttttgc tatgctgcac ggccgtttgc cgcctgctcc
     6721 accccccgcc gtgcccgatc cgcttcgcgc cctcgccggg ctgctgccgc agtgcctcgc
     6781 tgtcgatcgc gaggatttcc gccgtcggct cgatcgcctc gaacgccgtc gtgacgacgg
     6841 ccgggcgcac gagcgcggcc tcgccgaact gatcacggcg atggaagcct cgcatgcgcg
     6901 gctgctcgaa cgtcgcgcgc gcctgccgct gccggtcttc gacgatgccc tgccggtcaa
     6961 ccagcgtcgc gaagacatcg cggcgctgat ccgtgcgcac caggtcgtcg tcgtctgcgg
     7021 cgagaccggt tcgggcaaga ccacgcagct gccgaagatc tgcctcgaac tcggcttcgg
     7081 ggcggccggg ctgatcggtc acacgcagcc gcgccggatc gccgcacgct cggtcgccgc
     7141 gcgcatcgcc gacgaactcg gggccggcgc ggcagcggcg gtcggctaca aggtgcgctt
     7201 ctcggatcgc accgggccgc aggccgcgat caagctgatg accgacggca tcctgctcgc
     7261 cgagacccag ggcgatccgc tgctgtcggc ctacgaggtg ctgattgtcg acgaggcgca
     7321 cgagcgcagc ctcaacatcg atttcctgct cggctacctg aaacgcctgc tgccgcggcg
     7381 gccggatctg aagctcgtca tcacctcggc gaccatcgac ccgcagcgct tcgcgcggca
     7441 tttcgacgat gcgccggtca tcgaggtgtc cgggcgcacg tatccggtcg aacagcgctg
     7501 gcggccgctg atcggcgagg acgaggacgc gcgtgatcgc gacctgcagc aggcgatcct
     7561 cgacgccgtc gacgaggtct gggccgaggg gccgggcgac gtgctggtgt tcctgtccgg
     7621 cgaacgcgag atccgcgaga ccgccgaggc cttgcgcaag caccatccac ccggcgtcga
     7681 gatcgtgccg ctgtacgcgc ggctgtcggc cgccgagcag aaccgcgtgt tcgaggcgca
     7741 cggccggtcg cgcatcgtgc tggcgaccaa cgtcgccgag acctcgctga ccgtgcccgg
     7801 catccgctac gtgatcgatc cgggcacggc gcgcgtctcg cgctacagct atcgcgccaa
     7861 gatccagcgc ctgccggtcg agaagatctc gcaggcgagc gcccgccagc gcgccgggcg
     7921 ctgcgggcgc gtcgaggccg gcgtctgcct gcgcctgtat tcggccgagg atttcgccgc
     7981 gcgcccggcg ttcaccgatc cggagatcct gcgcaccaac ctcgccgccg tgatcctgca
     8041 gatggcggcg ctgaacctcg gggacccggc cgaattcccg ttcgtcgagc cgccggattc
     8101 gcggctgatc cacgacggct acaagctgct gcacgaactg caggcggtcg acgagcgcca
     8161 ccggctgacc gcgctcgggc gcgaactggc gaagctgccg atcgacccgc ggctcgggcg
     8221 catgatcctc gcggcgcgcg acgagggctg tcttgccgag gtgctggtga tcgcggccgg
     8281 tctggcgatc caggacccgc gcgagcgccc gctcgaagtg cagcaggctg ccgaccagca
     8341 gcaccggcgc ttccgtgacg agcggtccga tttcctcgcg ctgctgcggc tctgggactg
     8401 gtggcaggcg cagggccggc gcctgagcaa gagcaagctg cgtgaactcg cgaaggcgtc
     8461 cttcctttcc tatgtgcgca tgcgcgaatg gcgtgagctg cacggccagc tggtcgaggt
     8521 gctggccacg ctcggcgcgg tgcagaacga acgcgaaccg gccccgccgc cgcccgagcc
     8581 gaaccggaag cgcgaggacg gcgactccct ctggccggcc aacgaagtcc ctatccaccg
     8641 cgcgctgctc gccggcctgc tcggccacac cggcctgcgt cagcaggagg gcagcgagtt
     8701 cctcggcgcg cgtggccggc gcttccgcat ctggccggga tcgggcctga cgaagaagcc
     8761 gccgcgctgg ctgatggctg ccgagctggt cgagaccacg caggtctggg cgcgcacctg
     8821 cgcgcgcatc cagccggaat ggctggagac gctggccgcg cacctgatca agcgcgcgta
     8881 cagcgagccg cactgggaga agcgcgccgc gcaggtggcg gcgaccgagc gcgtgacgct
     8941 gtacggcctg ccggtcgtca ccggccgcaa ggtcaactac gggccgatcg atccgccgct
     9001 cgcgcgcgag ctgttcctcc gccacgcgct ggtcaacggc gagttcgact gccgcgcgcc
     9061 gttcgtcgcc cacaacgccg cgctgatcgc cgaaatcgcc cgcatcgagg acaaggcgcg
     9121 ccggcgcgac gtgctggtcg acgaggaggc aatcttccgc ttctacgatg cgcgcgtgcc
     9181 cgagcgggta tggtcggggg cggccttcct gaagtggctg aaggccgccg ggcgtgagga
     9241 tccacaactg ctgttcctga gccgcgagac gctgatggca cacgcggccg agcaggtcac
     9301 cgatgcgcgt tttccggatc ggctcgtgct cgccggcatc gagtttccgc tcgcctaccg
     9361 cttcgagccg ggtgcggccg acgacggcgt gacgctgacc gtgccgctcg ccgcgctcaa
     9421 ccaggtgccg gcggcgcgct gcgaatggct ggtgccgggg ctgctgcccg agcggctggc
     9481 ggcgctgctg aagacattgc cgaaggagct gcgccggcgg gtggtgccgg tgccggatac
     9541 cgtcgaaaag ctgctgcccg aactcgacgc gcacgccgat gcggaggcgc cgctgctgcc
     9601 ggcgctggtc gcggcgctgc gccggctcac cgccagcgag ttcccggagg atgccttcga
     9661 tccggctgcg ataccggcgc atctgggcat gaacttccgc gtcgtcgcgc ccgatggcgc
     9721 cgtgctcgcc gagggccgtg acctgcgccg gctgcgcgcg cagttcggtg atgcggcgcg
     9781 cgaggccttc ggcacgctgg cgagcgcctt ccgggccgcg gccgacaagg cgaggacacc
     9841 gctgcgcgcg cgcggcgaac cggtcattcc ggcatcgccg gcaccggcgc cgaagccggc
     9901 gatgccggcc gaggatccgg tcgcgcgtct ggagcgcgcc gaggtccacg actgggactt
     9961 cggggcgctg gcacagacca tcgagttcgt gcgcaacggc atcgtgctga agggctggcc
    10021 ggcgctggtc gtcgaaggcg agagacttgc cgtgcgcgtg ctcgataccg aagcgcgcgc
    10081 ggcggccgcc caccgtcacg gcctgcaccg gctgatcagc ttgcgcctgc gcgagccggt
    10141 caagtacctg cgccgcaacc tgccggggct cgcgcagatg ggcttgcgct acgtggcgat
    10201 cggcacgcag gaacagctgc gcgacgatct gctcgccgcg gcgatcgatc gcgcctttct
    10261 ggtgccggcg cttgccggcg gcgggctgcc gcgcgatcgg gcgggcttcg aggccatgct
    10321 ggagcacggc cgggcgcagt tcgtcggctg cgcgaacgag gtctgcgcgc aggtcgggca
    10381 ggcgctcgcg agcttccacg ccgtgcgccg gcgcatcagc ggccaggtgc cgctcgcgtg
    10441 gatggaagcg gcgaaggaca tgcacgagca gctcgacgcg ctggtctacc ccggcttcgt
    10501 cagcgcgacg ccgcccgaat ggctgcgccg gctgccggta tacctgaagg cgctggagct
    10561 gcgcgtcgag cgtctcggcg gccggctgga gcaggaccgc cagcgcagcg ccgaggtgca
    10621 gcggctgtgg cgctggtatc tgcagcgccg cacccagctc gcgcgccgcg gtgccgatga
    10681 tccggagctc gaacgctacc gctggatgct ggaggaactg cgcgtgtcgc tgttcgcgca
    10741 ggagctgaag acgctgtttc cggtgtcggt gaagcgcatc gaggagcagg gcgcgaaggt
    10801 ccgcgtctga gccccgccgc tcacggccgc cacagcacgc ccgggccgat gtcggcgagc
    10861 cgcgcgcagc cggtcagcgc catcgccact tcgagttcct cgcgcagcat cttcagcacg
    10921 tgcgcgacgc cgagcgcacc ggcggcggcg aggccgtgca gataggggcg gccgatcagc
    10981 acggcgctgg cgccgagcgc gagcgccttc agcacgtcgg tgccgcggcg gatgccgccg
    11041 tcgacgagga tcggcacgcg cccggcgacg cgctcggcaa tcaccggcag cgcctcggcg
    11101 ctggccggca aggtatcgag cacgcgcccg ccgtggttcg agacgatcag gccggcgacg
    11161 ccgcgttcga tcgcgagcgc ggcatcttcg gggctgacga tgcctttgag gatcaccggc
    11221 aggcgggtct ggtcgagcag ccagtcgagt tcgcgccagg tcggggccgt gcgcatgatg
    11281 ccgtcgaaca cgaggctggc ctgcgcgctg ccgggcgcga acggcgtctg gccgtggcgc
    11341 aggttggcgg cgctgacccc gggcggcagc cggaagccgg cacggcgttc gcggttgcgg
    11401 atgccggcga gcggcgcatc gacggtgacc accagcgccg aataaccggc ggcctcggcg
    11461 cgctgcacga gttcaacact cgccgcgcgt tcgggctggc agtagagctg gaaccacagc
    11521 ggcggcgcat cgggcagcgc cgcgaccgct tcgagctcga cgctcgcgag cgtgctcagc
    11581 acgaagccgg ccccgagtgc tgccgcggcg cgcgccgcgg ccagctcgcc gtccgggtgc
    11641 gcgagccgct ggtaggcgac cggtgccagc agcaccggat gttcgagccg gctgccgaac
    11701 agcgtcagcg ccgtgtcgcc gccggccacg tcggcgagca cgcgtccgtg cagccgcagc
    11761 cgctcccagg cctcgcggtt ccagcgcgca ctgagttcgt ccccggctgc gccgtcgaga
    11821 taggcccaga tcgccggatc gagccgttcg cgggcgtatg tggcgtagtc ggggacggcg
    11881 gcgatggcgt cggggatgcg ggtcagcagc ggcgggcggg acatcggcgg ggctcggcgg
    11941 caggcagatg aaaacgcatt ctaatcggga atgagtcgca tctgtcgcct gccgggcacg
    12001 cgatcggcag atccgcattg cgcaggaccc ggcagtcagg atgtccggat tccggacgcc
    12061 ggagcggccg gaacccgggg gctcggccgt cagcgggctg cgccgggcac ggatggcggc
    12121 cgccggcatc gtgccggcaa ggttgccgga gggtggggcg cgaagccgcg gccgccggtg
    12181 cagcggggcg ctgcgcccgg acggcggctg cgcatcaggc gccgtcgtcg ctgaccacgc
    12241 ggttgcggcc gccggacttg gcgcggtaca gacggcggtc gatgcgggtg aggaagtcgg
    12301 ccgggtcatc gaggcggtcg ggccggatgg caccgcagcc gatgctgacc gtcaccggca
    12361 ggcgcaggcc gtcgtcggtg gcgcggtact ggccttgcag ccgcgcggcg atgccctcgc
    12421 gcagcttgtc gacgacgcag tgcacgccgg cggcttcggt ttccggcagc agcagcgcga
    12481 attcctcgcc gccgacccgc gcgatcaggt cgccggggcg gcgcatggct tcgcgcccga
    12541 gcgcggcgat gcggcgcagg cagtcatcgc cggcgccgtg gccgcagcgg tcgttgaaca
    12601 gcttgaagtg atcggcatcg atcagcgcca gcccgagcgg acagccggtg cgcagcgccc
    12661 gccgccattc gcggtggtag tgctggtcga agcagcggcg gttggcgatg ccggtcaggc
    12721 cgtccatcag cgccagttgc tgcagcagct gcgtctggcg ccgcagggtc acgtgggtgc
    12781 gcagccgggc acgcaccacc ggcagtgcga acggccgcat gatgtagtcg accgcgccga
    12841 ggctgaagcc cttgatctgc gactcgacgt catcgacgcc ggtcatgaag atcagtggca
    12901 gctcgcggcc gaattcggtg tccttcaggc gccggcagac ctcgaagcca tcgacctcgg
    12961 gcatgtcgac atcgaggatc accagatcca gccgcgggct gtggcccacg atctcgacca
    13021 cctcccgcca gtgcttggcg aacagcagtt cgaactcgtc gccgagccct tcggccagca
    13081 gcacgtggtt gaacgggtca tcgtcgatga ccaggatgtg cggcatctcg gggctgctca
    13141 tggttcagcg tccggcggcc ggcgtcgtgg cgtggcggca cagccagtcg caggctgcgg
    13201 cctcgggcag cggtggcgag aagcggtaac cctgcaccgc atcgcagccg tgctcgatca
    13261 ggaaatcgag ctgctcgctg gtttcgacgc cttcggcgag caccgacagg ccgaggttgt
    13321 gcgcgagcgc gatgactgcg gcggtgatct gcatgtcggc cggatcgtgc gggatgtcga
    13381 gcacgaagct gcggtcgatc ttcagcgtgt tgatcgggaa gcgcttcagg taggcgagcg
    13441 aggaatagcc ggtgccgaag tcgtcgatcg cgaggctgac gccgagccgg cgcagggctt
    13501 cgagctgctg ggcgaccgtg tccgggtttt ccatcagcgc gctctcggtc agttcgagtt
    13561 cgagataccg ccccggcacg ccggtctcgt cgaggatggc cgcgagctgc ggcgccagcg
    13621 cgtgcaggcg cagctgccgg gccgacaggt tcacggccat cgagtggatc ggcacgccat
    13681 cggccagcca gcggcgcagg cagcggcagg cctcgcgcag cacccattcg ccgagcggca
    13741 cgatcagccc ggtttcctcg gcgagcggga tgaagtcgga cggcggcacc atcttgccgt
    13801 ccggccgccg ccagcgcacc agcgcctcga ccccgagggc cgccggttcg ccgagcatga
    13861 tctgcggctg gtagtacagc accagctccc cgcgttcgag cgcacggcgc aggcgcgcct
    13921 ccagcaccag gcgctcctcg acggcgcgcg tcatcgcctc cagatagaag cgcgcattgc
    13981 cgcgaccgtc ctccttggcg cgatacagcg cagtgtcggc acgctggatc agcgctccgg
    14041 cctcggcccc gtcggccggg tacaggctga tgccgacgct ggcaccgatc cagacctcgt
    14101 ggccgccgcc gagcaggaag ggctgctgca gggcttcgat cagcgtttgc gcgacgccgg
    14161 cggcacgctc gggttcggtg acctcctcaa gcacgaccac gaactcgtcg ccgccgaggc
    14221 gcgcgagcag gtcggactgg cgcagccgtt cgcgcagccg gccggccgcc cgcaccagca
    14281 gctcgtctcc ggcggtgtga ccgaagctgt cattgaccag cttgaagcgg tcgagatcga
    14341 gaaacagcac cgccaggccg cctccgctgc gcgcggcgcg gcgcagcgcc tggtcgagtt
    14401 cggcgatcag cagcagccgg ttcggcagtc cggtcagcgc gtcgtggtgc gcgaggtgtt
    14461 cgagatcgtg ggcgttgcgg accgccgtga tgtcggagaa cacgccgaca tagcggatga
    14521 tcgtctggtc ggcatcgcgc acggcgttga tcgtcagcca ctgcagatag ggttcaccgc
    14581 tgcgccggcg attgcggatc tcgccctgcc agtgcccgct ctgctgcagc gcgcgccaga
    14641 aatccagata gaacgcccgg ccctcgtagc cggactgcag catgcgcatg ttgcggccga
    14701 ggacttcgcc ttccgcataa cccgtgatgt tcgagaatgc cgggttgacg gcgaccaccg
    14761 tgccctcggg gtcgcagacg acgatgccgt cctgcgtgct cgaatagacc atcgccgcct
    14821 ggcgcagacc ggcttcgttg cgccggcgct cggcaatctc ggcctccagc gcctgcttgg
    14881 cgaggcgcag gtcgtgcgtg acgcgctcgt tctcggccag cgtcgcctcg agctcgagcg
    14941 tgcgctcgcg cacgtgatct tcgagcagcg cggccgtgtg atacagcgtg aagccggact
    15001 cggcgctctc caggctggtc gtcgatttct cggcgcgccg gatcagcgcc gtcaccacct
    15061 tgcgcagtcg caggacttcc gcctgcaggg tctcggggtc gtccggcagg ggcaggagtt
    15121 cattcatcac cggacctgcc gatcgcgacg ccggtcagcg tgtgattgac atgcacgccg
    15181 gcgtactgct cgccgtaggt gctgaacccg accatgcgat agcgccggaa gcgttcgccg
    15241 agcgcagcct cgcagtgctt gcgccgggct tcgagccggc gcagcacgca atcgaagctg
    15301 atgaccagat ccggttcgcc gatcccggcg cggatgctgt ccatcgacga gtcgaggtga
    15361 gcgaccatgt cgcgggcctg cgcgacgcgc agcaccagcc cgagttcgat cgcacagaag
    15421 aaggtcaggc tgccgtcggc gtgggcctcg cggatcgagc gcacgtaatc ggtgccgtcg
    15481 atgaccacga ccaccggtgc ggccgcgaag gtctcggggg caagctccgc caccggcacg
    15541 ccgatcaggc gggcatattc ctgcgccgcc ggcaggccgt tcagctcgaa cacccgccgg
    15601 cgcttcggat cggcctcggt gaccaccagg cgttccttgt cgtgggtgaa gtgctgggtc
    15661 ttgaacaccc ggctgcgccg gcgcgtgctg accagtgcca gcacggcgct gcgggtgtgg
    15721 aaggcgccat cgtgatagac ccagctgcgg ccgaagtgca gaccgtcacc ggccgagccg
    15781 ccgaccagcg gtgtgcgccc gagtgcgcgc tgcagcgcat aggcgaccgg ttcctcgcgc
    15841 tgcgcgaggc catcgaccag cagcagggcg aagcgctcct gcccgtccgt caccgccggc
    15901 atctgcgcga gcagggcatc gacacagtgc tggccggcat cgaggctgac gtcgtccaga
    15961 ccgtcgagcc gggcggtcgc caccgtgaat tctgccgccg gcaggctgaa gccggtgatg
    16021 ccgccggtct ggtagccgct ggcgccgagt tccccggcgg tcgtgcagcc gatcatcggc
    16081 acgtcaccga aatgatgctg cagggcttcg gccagcaccg gacgcggata ggcggtcgaa
    16141 cagaacacca cgaccagcgc cgcatcgggc tgactgatct gggcatgcag ttcgcgcacg
    16201 gccaggccga cctcgcacgc ctgcgtgtgg ccggtcagga tgcgggcgga agcggattcc
    16261 atcgatgact cct
//
LOCUS       DAOQIE010000041        16213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1687_length_16213_cov_8.107563, whole genome shotgun sequence.
ACCESSION   DAOQIE010000041 DAOQIE010000000
VERSION     DAOQIE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_16213_cov_8.107563"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..650)
                     /locus_tag="PK144_04230"
     CDS             complement(153..650)
                     /locus_tag="PK144_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64967.1"
                     /translation="MNKVLMTIPMVIVLAGAGLQDAQAAAYSTQDLAESFCLSTNNDL
                     FHVPSRDLGKTCSCVGVVANMNSYKGNLSETDAIMKGFHGCVNGNLISRYYEKLAWKA
                     TGSLTADFRKPFAECIGTQGVIRGTSFATNSRTYEQHLKGVMDSCQYIVGIDAFLRIL
                     SVLAR"
     gene            complement(1099..2820)
                     /locus_tag="PK144_04235"
     CDS             complement(1099..2820)
                     /locus_tag="PK144_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNF64968.1"
                     /translation="MSPGRRAGFWLPWLAVSRAEGAAGLVADAIAGLVVAILLVPQAL
                     AYAQLAALPPQTGLYAGLLAPLAYALCGASRVLAVGPVAVVSLMTAAAVGPVVAATGA
                     EPQAVALALALAVGLFSLGFALLGLGALAHFLSHPVIAGLMSASALLIAVGQARHLLG
                     IAVDGATLPVLLPGLLTRLDQVNPLALAFGAGAIALLVLAGRGLAPLLQRLGLAGPTA
                     RLLARLAPVFVVLLAIGLVRGFGLEHALRTVGDIPPGLPGLTLPPADPALWRAIAWPA
                     LLIAVIGFVESMAMAQTFAAPRRERIDPAQELRGLGLANLAAAFSGAYPVSGGLSRTV
                     IAAAAGARSPLAGVLSAVALVPALLWATPLLGALPQAVLAATIVVPILKLVDPPGLLR
                     LWRYSRADAAALAATGAGVLLLGVELGLLAGLAVSIGAFLLQASRPHYAIVGRVPGTE
                     HYRNVLRHPVAGSQRVLTVRVDESLRFCNAAWLEDTVAQLVAGRPELTDVVLMCPGVN
                     AIDASAFESLERIDARLRAGGLRLHLSEVKGPVMDRLAAAGLPQRLSGQVFLSQHQAM
                     QALEGGR"
     gene            complement(2817..3710)
                     /locus_tag="PK144_04240"
     CDS             complement(2817..3710)
                     /locus_tag="PK144_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF64969.1"
                     /translation="MSVPEVESFFEPFTETWSHLLIAPEGCCAILDPVLDYDYRSGRT
                     GTASAGRIAARVRERGLTVEWILETHVHADHLSAGDHLRRTLGGRLAIGEHVCEVQQR
                     FARLYGAGADFRSDGSQFDQLFTDGERFRLGSLEIEVLHGPGHTPACVAYRVGTAVFV
                     GDLIFMPDVGTARCDFPGGSARALYRSVRRILDLPPDTVLYFCHDYPPEGRERSAYAS
                     VAAQRAANIDIHDGIGEDEFVARRLARDAGMPLPRLMLPALQVNMRAGALPPAEADGV
                     QYLKIPLNVLGAPDAADDPQP"
     gene            complement(3878..4519)
                     /gene="queE"
                     /locus_tag="PK144_04245"
     CDS             complement(3878..4519)
                     /gene="queE"
                     /locus_tag="PK144_04245"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HNF64970.1"
                     /translation="MAYTVKELFYTLQGEGAQTGRAAVFCRFAGCNLWSGREADRARA
                     VCRFCDTDFLGTDGEGGGRFADAEALAQAVAARWPQGAGGRPYVVCTGGEPLLQLDAP
                     LIDALHARGFEVAVETNGTQPAPAGLDWICVSPKAGAPLVLTAGDELKLVYPQVEIEA
                     QPERFAALAFRHFFVQPLDGPERAGALAACIDYCQRHPQWRLSLQTHKLIGIP"
     gene            complement(4533..5642)
                     /locus_tag="PK144_04250"
     CDS             complement(4533..5642)
                     /locus_tag="PK144_04250"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF64971.1"
                     /translation="MSSPAVCYTLASAPFEAACRIPTLPATHPASRLHGHSYAARVRA
                     RLPADWAGFAGAGLGTLRECLGAAVAPLDYAYLNDHLPVPTDENLARWLRKALAGAGV
                     PGLESLGIRSTPDAGVDLDADGRAHVWRRFRFEAAHWLPKVPPGHPCGRLHGHGFEVV
                     LHANQDLGSADMGIDQDGLAVLWAPLGAKLDHACLNDLPGLDCPTSEVLARWIWQRLK
                     PRQPELSWVTVFETVTAGCQYDGEHFRIWKEQRFEAATRLSHAPAEDRRAGLHGHSYA
                     IRLHVDAPLDEVLGWTVDFGDVKARFAPVYDRLDHQRLDALPGLADGDTAALAAWIRA
                     EAAGALPELDRIDLYETPGNGVALCWGALAPALPG"
     gene            complement(5639..6358)
                     /gene="queC"
                     /locus_tag="PK144_04255"
     CDS             complement(5639..6358)
                     /gene="queC"
                     /locus_tag="PK144_04255"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNF64972.1"
                     /translation="MSQDPAPDTAPRALVVLSGGQDSTTCLYWALDRFGAGRVEALSF
                     DYGQRHRIELDCAARIAALADVPHVVLPINTFGALGGSALTEKAIPVRAGLEANRLPN
                     TFVPGRNLIFLSFAAAHAYRRGITELVTGVAQTDYSGYPDCRLATIEALQQAIRLGME
                     AEITIHTPLMFKSKAETVHMAQALGALPALAWSHTCYNGVQPPCGECPACRLRAQGFA
                     EAGIADPLIERLRGAGTDRHA"
     gene            complement(6518..7099)
                     /locus_tag="PK144_04260"
     CDS             complement(6518..7099)
                     /locus_tag="PK144_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64973.1"
                     /translation="MKIARPITMGCALICAVALAGCAATPEPPQPEVVAINATSASAR
                     VERVDQKQRQLTLKLQDGRRIELAVSPEVRNFAQIRRGDTVEVRYVESVLLQVHPKGT
                     AAPTASAGEYVQGAPAGEKPAGVAVQEVNIVAKIVAIAADRSMVTLQGPRGRQVDIAV
                     REPRRLDGVKVGDLVELTYRQGVAIEVSAPARK"
     gene            7397..8212
                     /locus_tag="PK144_04265"
     CDS             7397..8212
                     /locus_tag="PK144_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64974.1"
                     /translation="MRLPVPVPVLRRGLALLLLAAAAPPSPAAEAVETFTGIARLADG
                     SVYYVERHRVEWRGERPVTSFTRYSDAAGQSFGEMDSRFPAPTPAPYTPAYRFEDRRL
                     GRSDQLQIHGDRAETRAIAGDGSPEKSGNYALAPDLVSGQGLHFLLRARLAAFVDDPG
                     FELHARLLIPLDAAIYPLRVRRLALDDGRLTLRIDIDHWLYRLFAPHMDIAYDVRSRR
                     LLWYEGPTHILDALRAVRTVRIDYRYGSEAAAAGAIPDHHAAAGSTTSIAIGR"
     gene            complement(8125..8940)
                     /locus_tag="PK144_04270"
     CDS             complement(8125..8940)
                     /locus_tag="PK144_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF64975.1"
                     /translation="MERATEPALQRLRLLSYNIQSGLSTRRYSDYVTHSWRHVLPVPS
                     RMVNLDAIADLAAGYDIVGLQEVDPGSLRSGFVDQAAYLAARGGFPFVHDQSNRRIGM
                     ISHHANAVLSRHRPSAIEDRKLPGLIKGRGVLCVRYGDGPEALHLFIIHLALGRRGRM
                     QQLGFLAELIADARHAIVMGDLNCHAGSPEIAHLLARTSLAEPEALHLPTFPSWAPNR
                     QLDHILVSESIRVERVEVLRHTYSDHLPIAMEVVLPAAAWWSGIAPAAAASLP"
     gene            complement(9027..9695)
                     /locus_tag="PK144_04275"
     CDS             complement(9027..9695)
                     /locus_tag="PK144_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNF64976.1"
                     /translation="MPKFPLIPAVLLAFACALLPATASADEAPAFKEGTEYTVLKTPV
                     ATSTAEQPGKVEVVELFWFGCPHCYRLEPAIQEWLKARPEEAVFVRVPAALAPNWELG
                     ARAYYAALSLDVEDKLHQPLFDAIHKDRKLALITDENALVSFVSGLGIDAEAYRKALH
                     SFDVETRLRKSKQLVVQYRVTGVPAVIVAGKYVTDIAMAGSPARVMDVVNFLVRKESG
                     KSGS"
     gene            complement(9746..11065)
                     /gene="ccsB"
                     /locus_tag="PK144_04280"
     CDS             complement(9746..11065)
                     /gene="ccsB"
                     /locus_tag="PK144_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNF64977.1"
                     /translation="MLDVLGNGPESAPRRPATAEWIWIALVLTGAGWAGWTYGAVMDG
                     YELAILAGSALALIAWGWHWRPARAYSAAVAALSLFAIGRYGDSLGAAQTDFFLKYLL
                     ASQQAFMWMSALYVLATLTYAAALVSRSDFVGRTASALTWTASVMGLAGLMVRWRESY
                     LMGADIGHIPVSNLYEVFILFSVITALLYLYYEGRTRTRAMGAFVLLVISAAVGFLLW
                     YTFDREAQHIQPLVPALQSYWMKIHVPANFIGYGAFSLAAMLGVVYLLAQPQQAAPAL
                     RRVLWPAGALLVATGAAMVGWTFTHEEAAKVLRAWALLPVTAGLLVWVVAGQGRMADR
                     LPAPAVVDNILYKSIALGFAAFTVATILGALWAAEAWGGYWSWDPKETWALIVWLNYA
                     AWLHLRLTKGLRGPPMAWWAIIGLFVTAFAFLGVNMWLSGLHSYGTL"
     gene            complement(11097..13136)
                     /locus_tag="PK144_04285"
     CDS             complement(11097..13136)
                     /locus_tag="PK144_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNF64978.1"
                     /translation="MTAVRSARAARPALLSEFLGSMNLAITLLVVVAVASVIGTVLKQ
                     NEPYTNYVIKFGPFWFEVFRKLGLYDVYGAPWFLAILGFLVLSTGVCLWRHVPGMLAE
                     INHFRLTVQAKSLRAFHSHAEWTLAADPATVAAQAAGALRAQGYRVRSSTHEDGHTVL
                     AALRGSLNRFGYLFTHLAIVVICVGGLLDGNLPLKWKEWRGEVKLETRDVVARDVPPE
                     SRLAPGDSLSFRGNVRLPEDSSANFVFLRLRDGFFVQELPFAIELKQFRVEFYPTGQP
                     KNFESDVLIHDADRLKTPLASTIKVNHPLVYRGYAIYQSDFGDGGSLMRMTAQPLLGQ
                     SAAPPKLEGRIGEKLSLDTPQGPLTLELDDFRLYNILPVPASEQQAASDAVGEPGRKV
                     RNFGPNFTYRLRNAAGQALEFQNFMQPFRLDGRSFFLSGVRGTPSEEFSYLHIPADAQ
                     GTPGRFLRFTALLHDRARVQAVLARAGTTAPGMSAAELAAVRLRLVDLFLKQGIDGVI
                     EQTRQRVPAERAEEATRFFLEVLRMSLGELYVELLRGEGLAADAEPGPADEQFFNDAL
                     NALSAIPNYGAPFWLQLDGFEQVQASGLQITKAGGQNVVYLGFALLTAGVFVMFYTSH
                     RRVWVWLAPEDGGTRLILAGSGNRHQEAFVQEFERLEAAVAVQTGAATLPPNPPS"
     gene            complement(13265..13873)
                     /locus_tag="PK144_04290"
     CDS             complement(13265..13873)
                     /locus_tag="PK144_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF64979.1"
                     /translation="MKKLFLIAAASALIGSAPLALAAGDAAAGQAKSAMCGACHGADG
                     NSASADFPKLAGQNAGYLYKQLAEFKSGARVNPTMQGMVAALSDEDMQNLAAYFASQT
                     PGKGEADPALVKAGEALFRGGNLDTGVSACSACHGVSGKGNEGAKFPALAGQHAKYTE
                     VQLLAFRSMARANDPGQMMRGVAARMSDAEIKAIASYLQGLQ"
     gene            14091..14705
                     /gene="yihA"
                     /locus_tag="PK144_04295"
     CDS             14091..14705
                     /gene="yihA"
                     /locus_tag="PK144_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNF64980.1"
                     /translation="MNERYRSAHFLKSANELHQLPPDEGIEVALAGRSNAGKSSALNT
                     LTGQSRLARVSKTPGRTQLINLFELDAGRRLVDLPGYGYAQVPEAVKRHWQAVLARYL
                     EQRQALAGLLLVMDVRHPFNPLDRQMLDWTCHRGLATHVLLTKSDKLSRGAAASTLAQ
                     VRRELAAQYPNAGAQLFSSLDRSGLDAAHARLDAWFGFGDAAGS"
     gene            complement(14775..>16213)
                     /locus_tag="PK144_04300"
     CDS             complement(14775..>16213)
                     /locus_tag="PK144_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF64981.1"
                     /translation="ELALAEVHRELEARVAARTQALNRAVQDLHVEIEERRQAEARAR
                     HLAHHDALTGLPNRALFGERLERAIAAHLAGQTAPGARIALLFLDLDRFKTINDTLGH
                     GYGDELLRSLARRLPVCVREQDTVARLGGDEFVVLLNGIEDPQAAAQVAERILASVCE
                     PMRIGGHELQVTPSIGISLYPDDGQDAATLMKHADLAMYEAKSQGRNGYRHYAPRLNR
                     QAGTRLRLAGELRHALARDEFVLFCQPRARLADGAVTGLEALLRWRHPQLGTLRPAAF
                     LGAAEELGLVPELLDWLLDATCRQSAAWLQAGLPVTTVAVSLPAQRYRLPALAPRVQQ
                     ALDASALAPKHLELQIDERLLAAQPVEIDALARRLDAIGVRLALDGFGIGRANLDCLV
                     RLPLARVTLPPRFVRALNASAPATHAIVRASVAIARELGLGTLAEGVEQRGQLDVLKA
                     AGCEEYQGGLLARPMAAGRFEAWLKARG"
BASE COUNT         2478 a         5726 c         5762 g         2247 t
ORIGIN      
        1 ctgccgatga ccgaaagacc tgctccgagc gcaaacggat cgtccggccg gagccggacg
       61 atttctcggg catctgtgat ccctgctgga ttttcacggt tttccaaagg ccgggcagcg
      121 cggcccggcc acggtacggc ctgcccctgc agtcaacgcg cgagaaccga caggatccgc
      181 aggaaggcgt cgataccgac aatatactga caactgtcca tgacgccttt cagatgctgc
      241 tcataggtcc ggctgttggt cgcaaatgaa gtaccacgga tcaccccctg agtcccgatg
      301 cactccgcaa acggtttgcg aaaatcggcc gtgagactgc ccgtggcttt ccatgccagc
      361 ttttcatagt aacgggaaat cagattgcca ttcacacagc catgaaagcc tttcatgatg
      421 gcatcggttt ccgacaggtt acccttataa gaattcatgt tcgcgacgac gccgacacat
      481 gagcaagtct ttcccaggtc ccgggacggc acatgaaaca gatcattatt cgtgctcagg
      541 cagaaggatt ccgccagatc ctgcgtcgaa tacgcggcgg cctgagcgtc ctgaagaccc
      601 gcaccagcca gaacgattac catggggatc gtcatcagca ctttgttcat gtctgtttca
      661 tcctgtatgc cgggtgatgg gcaaaacttt gttcctggca tcacgagcac gacggggaat
      721 atcgaccggt ccctggcccc gtatgatccg gactgcactt ccgtaatcca tcgcaacgct
      781 cgtcggccgg atggccgtag gccacaacgc catgttcatc ggcgtcaaaa cctctgacgg
      841 atggaggagc tgcgactggc agcattaatt gttaccagat gtttcatctg gctgtggccg
      901 gagtaccgcc ggaatacaac cggactccgg acagcacgga tgcggacggc tgtgacagcc
      961 gatgcggcag ggggtctgga agacggacag atttgccggc gctgccactc acacgcgggt
     1021 cagcgatttc tgccgtgcag gccgggcatg ccgctgcggg cgcggcctgc gaagcaggcc
     1081 atcagcgggc tgctgccgtc accgcccgcc ttccagcgcc tgcatcgcct gatgctggct
     1141 caggaacacc tgccccgaca ggcgctgcgg caggccggcg gcggcgaggc ggtccatgac
     1201 cgggcccttg acctcggaaa gatgcaggcg caggccgccg gcgcgcaggc gggcgtcgat
     1261 gcgttcgagg ctttcgaagg cgctggcgtc gatggcgttg acgcccgggc acatcagcac
     1321 cacgtcggtg agttccggcc ggccggcgac gagctgcgcg accgtgtcct ccagccacgc
     1381 ggcattgcag aagcgcaggc tctcgtcgac gcgcacggtc agcacccgct ggctcccggc
     1441 gaccgggtgg cgcagcacgt tgcggtagtg ctcggtgccg ggcacgcggc cgacgatcgc
     1501 gtaatgcgga cggctcgcct gcagcaggaa ggcgccgatc gacaccgcga gaccggcgag
     1561 caggcccagc tcgacgccga gcagcagcac gccggccccg gtcgcggcca gggccgccgc
     1621 atcggcacgc gaatagcgcc acagccgcag cagccccggc gggtcgacga gcttcaggat
     1681 cggcacgacg atggtcgcgg ccagcaccgc ctgcggcagc gcgccgagca gcggcgtggc
     1741 ccacagcagc gccggcacga gcgccacggc gctcagcacg ccggccagcg gcgaacgggc
     1801 accggccgcc gccgcgatca cggtgcgcga cagcccgccc gaaaccggat acgcaccgct
     1861 gaaggccgcg gccagattcg cgagcccgag gccgcgcagt tcctgcgccg gatcgatgcg
     1921 ctcgcgccgc ggcgcggcga aggtctgcgc catcgccatc gattcgacga aaccgatcac
     1981 cgcgatcagc agcgccggcc acgcgatcgc gcgccagagt gccggatcgg ccggcggcag
     2041 cgtcaggccg ggcagaccgg gcgggatgtc gccgaccgtg cgcagcgcgt gttcgagccc
     2101 gaaaccgcgc accagcccga tcgcgagcag cacgacgaac accggcgcca gccgcgcgag
     2161 cagccgggcg gtcgggccgg cgaggccgag gcgctgcagc aagggggcga gcccgcgtcc
     2221 ggcgagcacc agcagcgcga tcgcgccggc gccgaaggcg agcgcgagcg gattcacctg
     2281 gtcgagacgg gtcagcagac ccggcagcag caccggcagc gtcgccccgt cgacggcgat
     2341 gccgagcagg tgtctggcct gaccgaccgc gatcagcagc gccgaggcgc tcatcaggcc
     2401 ggcgatcacc ggatgactca ggaagtgcgc gagcgcgccg agcccgagca gtgcgaagcc
     2461 gagcgagaac agcccgaccg cgagcgcgag cgcgagcgcg accgcctgcg gctcggcgcc
     2521 ggtcgcggca accaccggcc cgaccgcggc ggccgtcatc agcgacacga cggcgaccgg
     2581 cccgaccgcg agcacgcggc tcgcgccgca gagcgcgtag gcaagcggtg ccagcaggcc
     2641 ggcatacagc ccggtctgcg gcggcagggc ggcgagctgc gcgtaggcca gcgcctgcgg
     2701 caccagcagg atcgcgacca caaggccggc gatcgcatcg gccaccagcc ccgccgcgcc
     2761 ttcggcccgg ctgacggcga gccacggcag ccagaacccg gcccgccgcc cggggctcac
     2821 ggctgcggat cgtcggctgc gtccggggcg ccgagcacgt tcagcggaat cttcaggtac
     2881 tgcacgccgt cggcttcggc cggcggcagc gcaccggcac gcatgttgac ctgcagcgcc
     2941 ggcagcatca gccgcggcag cggcatgccg gcatcgcggg cgaggcgccg ggcgacgaac
     3001 tcgtcctcgc cgatgccgtc gtggatgtcg atgttggcgg cgcgctgtgc ggcgacgctc
     3061 gcgtatgccg agcgttcccg gccctcgggc ggatagtcgt ggcagaagta caggaccgtg
     3121 tccggcggca ggtcgaggat gcggcgcacc gagcggtaca gcgcccgcgc cgagccgccg
     3181 gggaagtcgc agcgcgcggt gccgacatcg ggcatgaaga tcaggtcgcc gacgaacacg
     3241 gccgtgccga cccggtaggc gacgcaggcc ggcgtgtggc cgggaccgtg caggacctcg
     3301 atctccagcg agccgagccg gaaacgctcg ccgtccgtga acagctggtc gaactgcgat
     3361 ccgtcgctgc ggaaatcggc cccggcgccg tagagccgcg cgaagcgctg ctgcacctcg
     3421 cacacgtgct cgccgatcgc cagccgcccg ccgagcgtgc gccgcagatg gtcgccggcc
     3481 gacaggtgat cggcgtgcac gtgggtttcg aggatccact cgaccgtcag cccgcgttcg
     3541 cgcacgcgcg ccgcgatgcg cccggcgctg gccgtgccgg tgcgccccga gcggtagtcg
     3601 tagtcgagca ccggatcgag gatcgcgcag cagccttcgg gcgcgatcag caggtgcgac
     3661 caggtttcgg tgaacggctc gaagaagctt tcgacctcgg gcacagacat ggcggcatcc
     3721 ggcaggggga ggacgtgaat atattcaaat aatcgaatat acgcaaagtc agcaccgccg
     3781 gctgccgcgg ggccggccgg cattcttcgc cgcaagggac agccggccgc gcccgcggga
     3841 tcgggcagtc agggactcgc cggtgcgagc ggccgcgcta cggaatgccg atcagcttgt
     3901 gcgtctgcag gctcagccgc cattgcgggt ggcgctggca gtagtcgatg caggcggcga
     3961 gcgcgccggc gcgctccggg ccgtcgagcg gctgcacgaa gaagtgccgg aaggcgagtg
     4021 ccgcgaagcg ctcgggctgt gcctcgatct ccacctgcgg gtagacgagc ttcagctcgt
     4081 cgccggccgt cagcaccagc ggcgcgccgg ccttggggct cacgcagatc cagtcgaggc
     4141 cggccggggc cggctgcgtg ccgttggtct cgaccgcgac ctcgaagccg cgcgcgtgca
     4201 gcgcgtcgat cagcggcgcg tcgagctgca gcagcggctc gccgccggta cagaccacgt
     4261 agggccggcc gcccgcgccc tgcggccagc gcgcggcgac ggcctgcgcg agggcttcgg
     4321 cgtccgcgaa gcgcccgccg ccttcgccgt cggtgccgag gaagtcggtg tcgcagaagc
     4381 ggcagacggc gcgcgcgcgg tcggcctcgc gcccgctcca cagattgcag ccggcgaagc
     4441 ggcagaacac cgccgcgcgg ccggtctgcg cgccctcgcc ctgcagcgtg tagaacagtt
     4501 ccttgaccgt gtaggccatc gtgcgtgccc gctcagcccg gcagcgccgg ggcgagcgcg
     4561 ccccagcaga gcgccacgcc gttgccgggc gtctcgtaga ggtcgatgcg atcgagttcc
     4621 ggcaaggccc cggcggcctc ggcgcggatc cacgcggcga gcgcggcggt atcgccatca
     4681 gcgagccccg gcagtgcgtc gagccgctgg tgatcgagcc ggtcatagac cggcgcgaac
     4741 cgcgccttca cgtcgccgaa gtcgacggtc cagccgagca cctcgtcgag cggtgcgtcg
     4801 acgtgcaggc ggatcgcgta gctgtggccg tgcaggccgg cgcggcggtc ctccgccggc
     4861 gcgtgcgaca gccgggtggc ggcctcgaag cgctgttcct tccagatgcg gaagtgctcg
     4921 ccgtcgtact ggcagccggc ggtgacggtc tcgaacaccg tcacccacga cagctccggc
     4981 tgccgcggtt tcagccgctg ccagatccag cgcgccagca cctcgctggt cgggcagtcg
     5041 aggccgggca gatcgttcag gcaggcgtgg tcgagcttgg caccgagcgg tgcccacagc
     5101 acggcgaggc cgtcctggtc gatgcccatg tcggcgctgc cgagatcctg gttggcgtgc
     5161 agcacgacct cgaaaccgtg gccgtgcagc cgcccgcacg gatggccggg cggcaccttc
     5221 ggcagccagt gcgcggcctc gaagcggaag cgccgccaga cgtgtgcgcg gccgtcggca
     5281 tcgagatcga cgccggcatc cggcgtcgag cggatgccga gcgattccag ccccggcacc
     5341 ccggccccgg ccagcgcctt gcgcagccag cgcgccaggt tctcgtcggt cggcacgggc
     5401 agatgatcgt tcaggtacgc gtagtcgagc ggtgcgaccg ccgcgcccag gcattcgcgc
     5461 agcgtgccga ggccggcacc ggcaaagccc gcccagtccg ccggcagtcg cgcgcgcacg
     5521 cgcgcggcat agctgtggcc gtgcaggcgg ctcgccgggt gcgtggccgg cagcgtcggg
     5581 atgcggcagg cggcctcgaa cggcgcgctc gcgagcgtgt agcagacggc cgggctgctc
     5641 atgcgtgccg gtcggtgccg gcgccgcgca ggcgctcgat cagcggatcg gcgatgccgg
     5701 cctcggcgaa gccctgcgcg cgcagccggc aggccgggca ctcgccgcag ggcggctgca
     5761 cgccgttgta gcaggtgtgg ctccacgcca gcgccggcag cgcgccgagc gcctgcgcca
     5821 tatgcacggt ctcggccttg ctcttgaaca tcagcggcgt gtggatcgtg atctccgcct
     5881 ccatgccgag acggatcgcc tgctgcagcg cctcgatggt cgcgagccgg cagtccggat
     5941 aaccggagta gtcggtctgc gcgacgccgg tcacgagttc ggtgatgccg cgccggtagg
     6001 cgtgtgccgc ggcgaagctc aggaagatca ggttgcgccc gggcacgaag gtgttcggca
     6061 gccggttggc ttccagcccg gcgcgcaccg ggatcgcctt ctcggtcagc gcgctgccgc
     6121 cgagggcgcc gaaggtgttg atcggcagca cgacgtgcgg cacgtcggcg agcgcggcga
     6181 tgcgcgccgc gcagtcgagt tcgatgcggt ggcgctggcc gtagtcgaag ctcagcgcct
     6241 cgacccggcc ggctccgaag cgatcgagcg cccagtacag gcaggtggtc gaatcctggc
     6301 caccggagag cacgaccagc gcgcgcggcg cggtatcggg agcgggatcc tgggacatcg
     6361 gcggactcga tcgggggaaa gaccggctgg ataccccgat tgccggcagc gttcaagtcc
     6421 gcatcgcccg ggtgccggcc cggtggccgc ttacctgcac tgcgtgcgtc cgttcagccc
     6481 ggcgtccctg ccggatgcag ggcggcagcc tcgttcgtca cttgcgcgcg ggggcactga
     6541 cctcgatcgc cacgccctgg cggtaggtca gctcgaccag atcgcccacc ttgacgccgt
     6601 cgagccggcg cggctcgcgc acggcgatgt cgacctggcg cccgcgcggc ccctgcagcg
     6661 tcaccatgct gcgatcggcg gcaatcgcga cgatcttcgc gacgatgttg acctcctgca
     6721 ccgcgacccc ggccggcttc tcgccggccg gtgcgccctg cacgtactcg ccggcgctgg
     6781 ccgtcggcgc ggccgtgccc ttcgggtgaa cctgcagcag caccgattcg acgtagcgca
     6841 cctcgacggt gtcaccacgg cggatctgcg cgaagttacg cacttccggg gaaacggcca
     6901 gctcgatgcg gcggccgtcc tgcagcttca gcgtcagctg ccgctgcttc tggtcgacgc
     6961 gctcgacgcg cgcactggcg ctggtggcgt tgatcgccac gacttccggc tgcggcggct
     7021 cgggggtggc ggcacagccg gccagcgcca cggcgcagat cagggcacag cccatcgtga
     7081 tcggccttgc gatcttcatc ggttcggaac tccatcggat acgggaatgc gcgccggcac
     7141 atgccgggcg tcgagcggga ccgcggccac cgcgccggcg gcagatcccg gcggtctgcg
     7201 gacggcgcgg gtccggcgca gcaacaacgg tgcgccgtgt gtttcacggc cggcgcagca
     7261 tagccgacgc actgcctgcg tgccggcccg cacctcgtgc gcacccacgc cggcagcgtc
     7321 cgtgtgcatc catcttcggc aggcccgcgc atcccgggtg gcggcaatcc ccctgcccgc
     7381 cgccggagcc cggatcatgc gcctgcccgt ccctgtcccc gtcctgcgtc gtggtcttgc
     7441 cctgctgctg ctggccgccg ctgcgccgcc gtccccggcc gccgaagcgg tcgaaacctt
     7501 caccggcatc gcccggctgg ccgatggcag cgtgtattac gtcgagcgtc atcgggtcga
     7561 atggcgcgga gaacggccgg tcaccagctt cacccgctac agcgatgccg ccggccagtc
     7621 cttcggcgag atggattcgc gctttccggc gccgaccccg gcgccataca cgccggcgta
     7681 tcgcttcgag gaccgccggc tcgggcgcag cgatcagctg cagatccacg gcgaccgggc
     7741 cgagacccgc gccatcgccg gtgacggcag tccggagaaa agcggcaact atgcgcttgc
     7801 gccggatctg gtcagcggcc agggcctgca cttcctgctg cgcgcgcggc tcgcggcctt
     7861 cgtcgacgat ccgggcttcg agctgcatgc gcggctgctg ataccgctcg atgcggcgat
     7921 ctacccgctg cgcgtgcgcc ggctcgcgct cgacgacggg cggctgacgc tgcgcatcga
     7981 catcgatcac tggctgtacc ggctgttcgc gccccacatg gacatcgcct acgacgtgcg
     8041 cagccgtcgc ctgctctggt acgagggacc gacgcacatc ctcgatgcac tgcgcgcggt
     8101 gcgcacggtg cgcatcgact accgctacgg cagcgaggcc gccgcggccg gcgcgatccc
     8161 ggaccaccac gccgcggccg gcagcacgac ctccatcgcg atcggcaggt gatccgaata
     8221 ggtgtggcgc agcacctcga cgcgttcgac gcggatcgac tccgacacca ggatgtggtc
     8281 gagctggcgg ttcggcgccc agctcgggaa ggtcggcagg tgcagcgcct cgggctcggc
     8341 cagcgaggtg cgcgccagca gatgggcaat ttccgggctg ccggcgtggc agttcaggtc
     8401 gcccatgacg atggcgtgac gggcatcggc gatcagttcg gccaaaaagc cgagctgctg
     8461 catgcgcccg cgccggccga gcgcgaggtg gatgatgaac aggtgcagcg cctcggggcc
     8521 gtcgccgtag cgcacgcaca gcacgccgcg gcccttgatc aggccgggga gcttgcggtc
     8581 ctcgatcgcg ctcgggcggt ggcgcgacag cacggcgttg gcgtgatgcg agatcatgcc
     8641 gatgcgccgg ttcgactggt cgtgaacgaa cgggaagccg ccgcgcgcgg ccagatacgc
     8701 ggcctgatcg acgaagccgc tgcgcaggct gccggggtcg acctcctgca ggccgacgat
     8761 gtcgtagccg gcggcgagat cggcgatggc gtcgaggttg accatccgtg acggcaccgg
     8821 cagcacgtgg cgccagctgt gcgtgacgta atccgaatac cggcgcgtgc tcaggccgga
     8881 ctggatgttg tagctgagca gccgcaggcg ctgcagtgcg ggctcggtgg ctcgctccat
     8941 gacatggctc ccggcgatgc cgggcggcgc cgccaccggc gccgcgcagc gcgacccgct
     9001 cagttatggc tcatgcggct catcaactag ctgccgctct tgccgctttc cttgcgcacg
     9061 aggaagttca cgacgtccat cacccgcgcc ggcgatccgg ccatggcgat gtcggtgacg
     9121 tacttgccgg cgacgatgac ggccggcacg ccggtgacgc ggtactgcac gacgagctgc
     9181 ttggacttgc gcagccgcgt ctcgacatcg aacgaatgca gcgccttgcg gtaggcctcg
     9241 gcgtcgatgc cgaggccgct gacgaagctg accagcgcgt tctcgtcggt gatcagcgcc
     9301 agcttgcggt ccttgtggat cgcatcgaac agcggctggt gcagcttgtc ctcgacatcg
     9361 agcgacagcg ccgcgtagta ggcgcgcgcg ccgagttccc agttcggcgc gagcgccgcc
     9421 ggcacgcgca cgaacacggc ctcctcgggc ctcgccttca gccattcctg gatcgccggc
     9481 tccagccggt agcagtgcgg gcagccgaac cagaacagct cgacgacctc gaccttgccg
     9541 ggctgttcgg ccgtcgacgt ggcgaccggc gtcttcagca ccgtgtactc ggtgccttcc
     9601 ttgaaggccg gggcctcgtc ggcggaagcg gtggccggca gcagcgcgca ggcgaaggcc
     9661 agcagcacgg cggggatcag cggaaacttc ggcatgcgga cgattccttc ggtacggggg
     9721 gaagacggtt cggcgccggg cgcgctcaca gcgtgccgta ggaatgcagg cccgacagcc
     9781 acatgttgac gccgaggaac gcgaacgcgg tcacgaacag gccgatgatc gcccaccacg
     9841 ccatcggcgg gccgcgcagg cccttggtca ggcgcaggtg cagccaggcg gcgtagttca
     9901 gccagacgat cagcgcccag gtttccttcg gatcccagga ccagtagccg ccccaggcct
     9961 cggccgccca cagcgcgccg aggatggtcg cgaccgtgaa cgccgcgaag ccgagcgcga
    10021 tcgacttgta gaggatgttg tcgaccacgg ccggtgccgg cagccggtcg gccatgcgcc
    10081 cctgaccggc gaccacccag accagcagcc cggccgtgac cggcagcagc gcccaggcgc
    10141 gcagcacctt cgcggcctcc tcgtgcgtga aggtccagcc gaccatcgcc gcgccggtgg
    10201 cgacgagcag cgcgcccgcc ggccacagca cgcggcgcag cgccggtgcg gcctgctgcg
    10261 gctgcgcgag cagatagacg acgccgagca tcgccgccag cgagaaggcg ccgtagccga
    10321 tgaagttggc cggcacgtgg atcttcatcc agtagctctg cagcgccggc accagcggct
    10381 ggatgtgctg ggcctcgcga tcgaaggtgt accagagcag gaagccgacc gcggccgaga
    10441 tcaccagcag cacgaaggcc cccatcgccc gggtgcgcgt gcggccctcg taatagaggt
    10501 acagcagcgc cgtgatcacc gagaacagga tgaagacttc gtagaggttg ctgaccggga
    10561 tgtggccgat gtcggcgccc atcaggtagg actcgcgcca gcgcaccatc agcccggcca
    10621 gccccatgac gctcgccgtc caggtcagcg cgctggcggt gcgtccgacg aagtcggagc
    10681 gcgacaccag cgccgccgcg taggtgagcg tcgccagcac gtacagcgcg ctcatccaca
    10741 tgaacgcctg ctggctcgcg agcaggtact tcaggaagaa gtcggtctgc gcggccccga
    10801 ggctgtcgcc gtagcggccg atcgcgaaca gcgacagcgc cgcgaccgcg gccgagtacg
    10861 cgcgcgccgg ccgccagtgc cagccccacg cgatcagcgc cagcgccgag ccggcgagga
    10921 tcgcgagttc gtagccgtcc atgaccgcgc cgtaggtcca gccggcccag ccggcgccgg
    10981 tcagcaccag cgcgatccag atccattccg ccgtggccgg ccggcgcggg gcgctttccg
    11041 ggccattgcc gagcacgtcg agcatgcgct cgcgggtcac cgccatgatc gtcacctcag
    11101 gatggaggat tcggggggag cgtcgcggca ccggtctgca cggcgaccgc ggcttcgagc
    11161 cgttcgaact cctgtacgaa ggcttcctga tggcggttgc cgctgccggc gaggatcagc
    11221 cgggtgccgc catcctcggg cgcgagccag acccagacgc gccggtgcga ggtgtagaac
    11281 atcacgaaca cgccggccgt cagcagcgcg aagccgaggt aaaccacatt ctggccaccg
    11341 gctttcgtga tctgcaagcc ggaagcctgc acctgctcga agccatcgag ctgcagccag
    11401 aacggcgccc cgtagttcgg gatcgccgac agcgcgttca gcgcatcgtt gaagaactgc
    11461 tcgtccgcgg ggccgggctc ggcatcggcg gccagaccct cgccgcgcag cagctcgacg
    11521 tacagctcgc cgaggctcat gcgcagcact tcgaggaaga agcgcgtcgc ctcctcggcg
    11581 cgttcggccg gcacgcgctg gcgggtctgc tcgatgacgc cgtcgatgcc ctgcttcagg
    11641 aacagatcga cgaggcgcag gcgcactgcc gccagttcgg ccgccgacat gcccggcgcc
    11701 gtcgtgccgg cgcgcgcgag cacggcctgc acgcgggcac ggtcgtgcag cagcgcggtg
    11761 aagcgcagga agcgccctgg cgtgccctgc gcatcggccg ggatgtgcag gtagctgaac
    11821 tcctcggacg gcgtgccgcg cacgccggac aggaagaacg agcgcccgtc gaggcggaac
    11881 ggctgcatga agttctggaa ctccagcgcc tgaccggcgg cgttgcgcag ccggtacgtg
    11941 aagttcggcc cgaagttgcg caccttgcgc cccggctcgc cgaccgcgtc gctcgcggcc
    12001 tgctgctcgg aggccggcac cggcaggatg ttgtagagcc ggaagtcgtc gagttcgagc
    12061 gtcagcggcc cctgcggcgt gtcgagcgag agcttctcgc cgatgcggcc ttcgagcttc
    12121 ggcggcgcgg cactctggcc gagcagcggc tgcgcggtca tgcgcatcag cgagccgccg
    12181 tcgccgaagt cggactggta gatcgcgtag ccgcggtaga ccagcgggtg gttgaccttg
    12241 atcgtcgagg cgagcggtgt cttcagccgg tcggcatcgt ggatcagcac gtcgctctcg
    12301 aagttcttcg gctggccggt cggatagaac tcgacgcgga actgcttcag ctcgatcgca
    12361 aacggcagct cctgcacgaa gaagccgtcg cgcaggcgca ggaacacgaa gttcgccgag
    12421 ctgtcctcgg gcaggcgcac gttgccgcga aacgacaggc tgtcgccggg cgcgaggcgg
    12481 ctctccggcg gcacgtcgcg cgcgaccacg tcacgggttt cgagcttcac ctcgccgcgc
    12541 cattccttcc atttcagcgg caggttgccg tcgagcaggc cgccgacgca gatcacgacg
    12601 atcgccagat gcgtgaacag gtagccgaag cggttgaggc tgccgcgcag cgccgcgagc
    12661 acggtgtggc cgtcctcgtg cgtgctgctg cgcacgcgat agccctgggc gcgcaacgcg
    12721 ccagccgcct gcgcggcgac cgtcgccgga tcggcggcga gcgtccattc ggcgtggctg
    12781 tggaaggcgc gcagcgactt cgcctgcacc gtcagccgga agtggttgat ctcggcgagc
    12841 atgcccggca cgtgccgcca caggcagacg ccggtcgaca gcaccaggaa gccgaggatc
    12901 gccagaaacc acggtgcacc gtagacatcg tacaggccga gcttgcggaa cacctcgaac
    12961 cagaacggtc cgaacttgat cacgtagttc gtgtacggct cgttctgctt cagtaccgtg
    13021 ccgatcaccg aagccaccgc gaccaccacc agcagcgtga tcgcgaggtt catcgagccg
    13081 aggaattcgg acagcagcgc cggacgggcg gcgcgggcgg aacggacggc agtcatcggg
    13141 cgctcgatgc gggcggtccg tcaccggacc gggagaaaag cgaaagggtg gcgccgggcc
    13201 acccttgttg cacgtatcgc acgtaactgc gtcggcggac tcccggcagg cgggagagcc
    13261 gggcttactg caggccctgc aggtacgagg cgatggcctt gatctcggcg tcgctcatgc
    13321 gcgccgcaac gccgcgcatc atctggcccg gatcgttggc gcgggccatc gagcggaagg
    13381 cgagcagctg gacctcggtg tacttggcgt gctggccggc cagcgccggg aacttcgcac
    13441 cttcgttgcc cttgccgctg acgccgtggc aggcggagca ggccgagacg ccggtgtcga
    13501 ggttgccgcc gcggaacagc gcctcgccgg ccttcaccag cgccggatcc gcctcgccct
    13561 tgcccggagt ctggctggcg aagtaggccg cgaggttctg catgtcctcg tcgctcagcg
    13621 cggcaaccat gccctgcatg gtcgggttga cacgcgcgcc ggacttgaac tcggccagct
    13681 gcttgtacag gtagccggca ttctggcccg cgagcttcgg aaagtccgcg ctcgcgctgt
    13741 tgccgtcagc accgtggcag gcaccgcaca tcgcggactt cgcctgaccg gcagcggcgt
    13801 cgcccgcggc cagggccagc ggggccgagc cgatgagggc gctcgcagcg gcaatcaaga
    13861 acagcttctt catgttgaaa cgactcctgt acgatgcggg attcggtgtt ctgaaacccg
    13921 gcggcccgcc cctggaaaca ggcttcgcgc cgggctctct cccctccccc ggcgggcacg
    13981 caaggggcga aaacagccgc gctatatacc agttccggca gggtcattca atcgcaagcc
    14041 ccgtcacagc ggtgctttgc cggtcgatcg ccgccggcgg attccggacc atgaacgagc
    14101 gttaccgcag tgcgcacttc ctcaagagcg ccaatgaact gcaccagctg ccgcccgacg
    14161 agggcatcga ggtggcgctg gccgggcgtt ccaacgccgg caagtcgagc gcgctgaaca
    14221 cgctgaccgg ccagtcgcgg ctcgcgcgcg tcagcaagac gcccgggcgc acgcagctga
    14281 tcaacctgtt cgagctcgat gccgggcgcc ggttggtcga tctgcccggc tacggctacg
    14341 cgcaggtgcc cgaggccgtg aagcgccact ggcaggcggt gctcgcgcgc tatctggagc
    14401 agcgccaggc gctggccggg ctgctgctgg tcatggacgt gcgccacccg ttcaacccgc
    14461 tcgaccggca gatgctcgac tggacttgcc accgcggcct tgcgacgcac gtgctgctga
    14521 cgaagtccga caagctctcg cgcggggccg cagcctcgac gctcgcgcag gtgcgccgtg
    14581 agctcgccgc gcagtacccg aacgccggcg cgcagctgtt ctcgtcgctc gatcgcagcg
    14641 gcctcgatgc cgcgcacgcc cggctcgacg cctggttcgg cttcggcgac gcggccggga
    14701 gctgaaagcc gggcgcgggc tgcgccagcc tccggccctc cttcgccctg ccctgcgcgg
    14761 gctgcggccc ggcctcagcc gcgcgccttc agccaggcct cgaagcgccc ggcggccatc
    14821 ggccgcgcca gcaggccgcc ttggtattcc tcgcagccgg cggccttcag cacgtcgagc
    14881 tggccgcgct gttcgacgcc ctcggccagc gtgccgagcc cgagttcgcg ggcgatcgcg
    14941 acgctggcgc gcacgatcgc gtgcgtggcc ggcgcgctgg cgttcagcgc gcgcacgaag
    15001 cgcggcggca gcgtcacgcg cgccagcggc aggcgcacca ggcagtcgag gttggcgcgg
    15061 ccgatgccga agccatcgag cgcgaggcgc acaccgatcg catcgagccg gcgtgccagc
    15121 gcgtcgatct cgaccggctg cgcggccagc aggcgttcgt cgatctgcag ctccagatgc
    15181 ttcggcgcca gggcgctcgc gtcgagcgcc tgctgcacgc gcggcgccag cgccggcagc
    15241 cggtagcgct gcgccggcag cgacaccgcc accgtcgtga ccggcaggcc ggcctgcagc
    15301 caggctgcgc tctggcggca ggtggcatcg agcagccagt cgagcagctc cggcaccagt
    15361 ccgagctcct cggctgcgcc gaggaaggcc gccgggcgca gcgtgccgag ctgcggatgg
    15421 cgccagcgca gcagcgcctc cagcccggtc acggcgccgt cggcgagccg cgcgcgcggc
    15481 tggcagaaca gcacgaactc gtcgcgcgcc agcgcatggc gcagctcccc ggccagccgc
    15541 agccgcgtgc cggcctgccg gttcaggcgc ggcgcgtagt ggcggtagcc gttgcgtccc
    15601 tgcgacttgg cctcgtacat cgccaggtcg gcgtgcttca tcagcgtcgc cgcatcctga
    15661 ccgtcgtccg gatacaggct gatgccgatg ctcggcgtca cctgcagctc gtggccgccg
    15721 atgcgcatcg gctcgcagac gctggcgagg atgcgctcgg ccacctgggc cgccgcctgc
    15781 gggtcctcga tgccgttcag cagcacgacg aactcgtcgc cgccgaggcg cgcgaccgtg
    15841 tcctgttcgc gcacgcagac cggcaggcgc cgggccaggc tgcgcagcag ctcgtcgccg
    15901 tagccgtggc cgagcgtgtc gttgatggtc ttgaagcggt cgagatcgag aaacagcagc
    15961 gcgatgcgtg ccccgggcgc ggtctggccg gccaggtgcg cggcgatcgc gcgctccaga
    16021 cgctccccga acagcgcgcg gttcggcagc ccggtcagcg cgtcgtgatg cgcgaggtgg
    16081 cgcgcgcgcg cctcggcctg acggcgctcc tcgatctcga cgtgcaggtc ctgcaccgcg
    16141 cggttcagcg cctgcgtgcg cgccgcgacc cgcgcttcga gctcgcggtg cacctcggcg
    16201 agcgcgagtt cgg
//
LOCUS       DAOQIE010000042        16161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1699_length_16161_cov_10.354713, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000042 DAOQIE010000000
VERSION     DAOQIE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16161
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_16161_cov_10.354713"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK144_04305"
     CDS             <1..342
                     /locus_tag="PK144_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF64982.1"
                     /translation="RLAGLRAAGRPVFVNLTAAWCITCLVNERTTLSSDTVRAAFAAG
                     GVAYLKGDWTRRDPAIGAYLASFGRDGLPFYAFYPAGGGEPRLLPPVLSPALVVEAIG
                     KPAAAPALVAR"
     gene            373..1083
                     /locus_tag="PK144_04310"
     CDS             373..1083
                     /locus_tag="PK144_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNF64983.1"
                     /translation="MSDASRSPAAPEARAPSQQPPACRRRGLLRGATALAVVLALGGG
                     AGTLLPEAKAAGPEVGRPAPAFTATDTQGRTVSLDGLRGRTVVLEWTNAECPYVGKHY
                     GADNMQKLQREATAAGVVWLSVISSAPGEQGYADAKEAERLNAERKAAPSAVLLDPDG
                     RLGHLYDARVTPHMFVIGPDGTLRYMGGIDSIPSARAADIDKAEPYFRNALHQVLDGQ
                     PVEKAVTRPYGCTVKYAS"
     gene            1180..1947
                     /locus_tag="PK144_04315"
     CDS             1180..1947
                     /locus_tag="PK144_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF64984.1"
                     /translation="MIDSPAFWLAAVPAVLIVGLSKGGFGGGLGVLGVPLLSLAVSPV
                     QAAAVLLPVLCVMDLASLWAFRHVPVARQLRKLLPGALLGILAGAWAWQHVDVAWVKL
                     IVGIEALAFTAHHVWQTRGGRQPPARAVSVTRAFGWGALAGFTSYLAHAGGPPLAACL
                     LPQRLPRAEFIGVTVFFFAVVNYTKLVPYALLGQLGDANLVTAAALLPLALVGVRLGV
                     VLHARLPEKPFYAICYAGLALTGTKLLWDALAALGGG"
     gene            2153..2863
                     /locus_tag="PK144_04320"
     CDS             2153..2863
                     /locus_tag="PK144_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNF64985.1"
                     /translation="MNLRVLMLVTSHDRLGDSGHPTGVWAEELAVPYYAFTDAGCHVD
                     LASPEGGPVPFDPRSLETADGPLPAAVERLQADEALREKLRQSWRAAAIDMHVFDALF
                     VPGGHGAMWDLATDPAVAHLVETAFASGRIVAALCHGPAALVSAKGPDGRFLLEGKHV
                     NSFTDAEEDALGLGDTVPFLLENRLRELGAFFEGGPNWQPYAVRDGNLITGQNPQSSA
                     LVAQQVLDALRAGLPAPA"
     gene            complement(2984..3787)
                     /locus_tag="PK144_04325"
     CDS             complement(2984..3787)
                     /locus_tag="PK144_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF64986.1"
                     /translation="MNARSRNRQGFVAGSLIGTLGGLIGLGGAEFRLPVLIGVFRLGT
                     LEAVILNKAMSLVVVTAALLFRGVTIAPAALGDHLDVMLNLLAGSLAGAWCAAGQAVT
                     LPRRWLDRIILVLLIGLSLLMLLETRAGLHEAGTPLLEPGPLRLVSGVFAGFAIGVVA
                     ALLGVAGGELLIPVIVLLYGLDIRLAGSLSLMVSLPTMMVGFARYTRAEAFSVLRQEM
                     PLMGSMVLGSILGAAVGALLLGRVPTDWLTGLLGIVLLVSALRMFPHAR"
     gene            4199..>6361
                     /locus_tag="PK144_04330"
     CDS             4199..>6361
                     /locus_tag="PK144_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HNF64987.1"
                     /translation="MSFAQLLQRYFYSHYVHSGLRVALGVLGLTLAAWATLGPHAAAA
                     VSSGALGTSLTDLPGPLAEKRRLMPAAVVLGTLATLGVGLSAGTPWLNAAVVLGITFV
                     SALLLGWGRAALPLSVGTLFVTAMALGSHEAAADPWQHAGWFLAGAGGYLLWGLVAAR
                     AAARRTRQQALAESLHELAEYLRAKADCYVPGADLERCYAQLVHRQTLLVERQQAARD
                     LVLEELRTPADGTLLATLLAAFDIYEAVLSAHTDHALLHRTLAGADALDFLHELVLKA
                     ARALDTVAAALMRDQPPPAATACFKPELYALAYERLRLDALAPTPDNQAARAALAATV
                     AKIERCVVRIQELPAIASRPVPPAEIFGGADFAAFRSPPLAGLKLLIAQIRAPRSPLL
                     RHALRLSAAVGAALALGRVLPYEAHGYWIVLTIALVLRPNFSITQQRQKDRLLGNLTG
                     CVLAAALLAAEPPAVLLLAAIFVANTVAHAFVTLRYRYTATAGCVIVLLQVHLTGSGA
                     GEVTMLARVLDTVLGGLLSIGASHLLPSWERYAMPALLRGLLDAAGAHARATLAEPMD
                     ELAYRLARKRYHDAVVALAGAFERMLAEPPDQQRAVAALERYVALGYLLGTQLVALHM
                     LRLHRGSEFEADAPERLRASAAAVAASLAAAAHAIDPAAVAPETLPPSANALADTLAD
                     DALPGDWHAERLLVRRLALIRASAGELRERAGALAAAGW"
     assembly_gap    6362..6461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6462..6780
                     /locus_tag="PK144_04335"
     CDS             <6462..6780
                     /locus_tag="PK144_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF64988.1"
                     /translation="RRRRPRAVPPHRSGAALAEVTAPATAGAGFPVACSLGKYSPVGN
                     LMGSLVGGNLWLEGGFARMMYLSLHKLHKLHKLALHGFWKVALDTLARMITRRTEPHV
                     KLH"
     gene            complement(6862..7173)
                     /locus_tag="PK144_04340"
     CDS             complement(6862..7173)
                     /locus_tag="PK144_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNF64989.1"
                     /translation="MTKFSVPSLEAVTAPIVAAGKLSVAQLEKLTSFQFALVQDYAEL
                     SLSRLKAATEISDLAGLKAYGEASVGVAKDVGAKVQADAKALVELLTGFGAEYKGLVR
                     V"
     gene            7531..8073
                     /locus_tag="PK144_04345"
     CDS             7531..8073
                     /locus_tag="PK144_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HNF64990.1"
                     /translation="MKAPLRPVLAAVALLALPALAVAKEYPVGKPVVKNGMEIAAVYL
                     QPVRMEPDGMMRKAEDSDVHLEADIKAAKNNPNGFAEGDWMPYLSVQYELSKQGGETQ
                     SGEFMPMVASDGPHYGDNVKLQGPGKYTLTLTISPPGANPHAHFGRHTDKETGVGPWF
                     EPFKVSYDFAFAGIGKKGGY"
     gene            8103..8465
                     /locus_tag="PK144_04350"
     CDS             8103..8465
                     /locus_tag="PK144_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNF64991.1"
                     /translation="MVRPSFLLRLHPGLRAVLGAALLAAAGAASAADAHTVTLIAKDG
                     RFEPATLEAPAGTRIRIEIENAGDTPIEFESTPLRKEKVLAPGARSVVVIQPLDPGRY
                     PFFDEFHPDTGRGELVVR"
     gene            8505..9917
                     /locus_tag="PK144_04355"
     CDS             8505..9917
                     /locus_tag="PK144_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF64992.1"
                     /translation="MNAAACPTGASPLATAVPASRLARIGLALRRHGRTIRMIQWAVV
                     FVYLALLLIPAALPLPPEDARLFGHLGTIAQFVFWGIWWPFVLISMVLFGRVWCGVFC
                     PEGALAEQASRVGLGRGIPRWLRWQGWPFVAFVLTTVYGQLVSVYQYPLAAALVLGGS
                     TVAAIAIGLLYGRNKRVWCRHLCPVSGVFSLLARLAPVHYRSDPERWTAKAGGPVQPV
                     NCAPLLDIRRLHGAAQCHACGRCAGQRGAVELTARAPSEEIVRHGAQDATRWDLRLVL
                     YGLCGVAMGAFHWTASPWFIDAKQAVAMWLLDHGGERLLDTDAPWWLLTHQPERHDVF
                     SWLDGLMIIAYIGATGLVVGAVLHLLCAIGARLLAQRPAPTAHLSLAFVPLAGAGVFL
                     GLSALTVSLLRGDGFALYWVNPLRELLLAGAALWTLRLGWGISGRYGAGLHRRLGATL
                     LVAAGAGFVVWGWALLFWLW"
     gene            complement(10018..10482)
                     /locus_tag="PK144_04360"
     CDS             complement(10018..10482)
                     /locus_tag="PK144_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF64993.1"
                     /translation="MLVPMSDRLNAYFDQLVQAGYRPRYDDDGDLVFHHDGFAIGISI
                     DEEDPEFLLVFIQNFHCSGTLTMQRVACDLMADLNRRFRLAKFSRRRQEVWVSAELLL
                     ARAGDFGPALPRLIELLASAARCFRKELRERMREIEHFDHALSAAAGLMLLH"
     gene            complement(10695..11495)
                     /locus_tag="PK144_04365"
     CDS             complement(10695..11495)
                     /locus_tag="PK144_04365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNF64994.1"
                     /translation="MNPETTTSTSNAASAAPVPAARPLAGKVAFVTGGARGIGAAIAQ
                     RLAADGAAVALTWARSEAEAAATVGTIEAAGGRALALRADSADDAALEAAIERTVAQF
                     GRLDILVNSAGVLALGSIEDFPLDGLDRTLAVNVRAVVIASRIAARHLGAGGRIITIG
                     STNAERMPFAGGAVYAMSKAAVAGLTKGMARDLGARGITVNTVQPGPVDTEMNPADGP
                     MAAGLLPMLATGRYGRAEEIASFVAYLAGPEAGYITGANLMIDGGFSA"
     gene            11640..12563
                     /locus_tag="PK144_04370"
     CDS             11640..12563
                     /locus_tag="PK144_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015476755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF64995.1"
                     /translation="MDTLNGLECFVRSAEARSFAAAGRRLGLTPAAVGKNVARLEATL
                     GVQLFRRSTRRLTLTEAGEDLLHAAAGPLEALNGALAQLAGSRSTPSGTLKVGLAPAF
                     GRDYILPLLGGFLARYPLIRPDWRFENRPVDLIGEGYDAAIGGGFELPPGAIARELAP
                     AHRVLLAAPAYLATHPAPQHPAELPCHTGILIRSLQTGRFPAWTLRDSHGAQAPVGLP
                     VSISVDDPEAACHAARAGLGITLASTAHALPFLEAGTLLRVLPDWHVDAGRIAIYFPA
                     QRLLAPKTRAFVDYVVEQCRAQRLAERLSAV"
     gene            12848..13693
                     /gene="trfA"
                     /locus_tag="PK144_04375"
     CDS             12848..13693
                     /gene="trfA"
                     /locus_tag="PK144_04375"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HNF64996.1"
                     /translation="MVDPVQRINALQERAAVTKPPALPRWEDWQRSVPNEVVRSALFT
                     VGNRRDRREFLVETELAAIGETQVTYQGVELRQDDEDVWLQALHLAREQPLDAPIEFV
                     PHAFLRAIGWPTTGHYYALLRDAFGRMQRTTVTFRNQRLHKGLSISLIRRFEWSDRDA
                     GGSDDRCKVWIEPEMRLLFGNTQYTRIEWEQRRHLRPLAKWLHGYFASHRTPLPVRVV
                     TLRDACGSDTRELRNFRPKLKAALTELVEACFLSDWQIDETDLVHVCRAPAPGVDACG
                     QTRLI"
     gene            13948..15162
                     /locus_tag="PK144_04380"
     CDS             13948..15162
                     /locus_tag="PK144_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF64997.1"
                     /translation="MSKPTGPAPDYSLTTDRLRALGEVSSKLIHELRAYSTSPEQRKL
                     LQKQWRIGEVAKLLGISESSLRNHDEALRGGEGNTGGAHRRYSLNDIHRLRELTHRQA
                     HRDPLRDRCARLAVCNFKGGSGKSTTATNLATHLVLHGYRTLFIDMDPQGSATALLYP
                     DNLMDIQRENTLYKVLFTQPERIRDLVIQTAWQGLDMIPAALSLFSVEAEILRYGFSN
                     PDFLFWERLDRALGHLQDDYDVILFDTPPSLSQTTVIAAWSATALVIPVQASMMDLQA
                     MASFFFGMWQSLSAIEQITGETKHYDFGLIVISNYRGNPKTMQDEYDVATWMRKMYQH
                     YVAEDAILNSSVIRSAANSIQIFYELPRRSDPSYARALGAFEGLGDQIIGLIRRQWPS
                     TQTAATRTASAS"
     gene            15176..>16161
                     /locus_tag="PK144_04385"
     CDS             15176..>16161
                     /locus_tag="PK144_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF64998.1"
                     /translation="MTSKPTRRKPRFEGLTLPSILDATPAAPGTAPAPAVPVADYVRE
                     AGQRLTEDYRRLRQLEQSGRVELELPLASIRPSRFANRSEHYFHSARYAEIRSSIAQH
                     GVKIPIKVRPVADSTPPAYEIVYGHTRHRASLDLGLPSIRAVVEALDDETLLAEMELE
                     NRDREDLSAYERARFYALALADTFAGNRERLCARFGRSNAWLSQQRVIAGLPQRLFDA
                     MPLLYQAGVEPLLELARLRPTDEQVQTLIEMHPDASSATTLVQVLIRRLHSASRGSPK
                     RTTASAEAVHHHRNGRLLMHARPGTRGRVQFEFESGQRGFADFLRTRLAELY"
BASE COUNT         2362 a         5879 c         5456 g         2364 t
ORIGIN      
        1 cgcctcgccg ggctgcgcgc cgccggccgg ccggtgttcg tgaacctgac cgcggcctgg
       61 tgcatcacct gtctggtcaa cgagcgcacg acgctgtcga gcgataccgt gcgcgcggcc
      121 ttcgcggccg gcggtgtcgc gtacctcaag ggcgactgga cgcgccgcga tccggcgatc
      181 ggcgcctatc tcgcgagctt cggccgcgac ggcctgccgt tctacgcctt ctatccggcc
      241 ggcggcggcg agccgcggct gctgccgccg gtgctgagcc cggcgctcgt ggtcgaggcg
      301 atcggcaagc ccgccgccgc gcccgcgctc gtcgcccgtt gacgaacccc gccaagcgag
      361 aggtgcaccg ccatgtccga tgcttccaga agccctgccg cccccgaagc ccgcgccccg
      421 tcgcagcaac cgccggcctg ccggcgtcgc ggcctgctgc gcggcgccac ggcgctcgcc
      481 gtcgtgctcg ccctcggcgg cggggccggc acgctgctgc ccgaggcgaa ggcggctggc
      541 cccgaggtcg gcaggccggc cccggcgttc acggccaccg acacgcaggg gcgcacggtc
      601 agcctcgacg gcctgcgcgg caggaccgtc gtgctcgaat ggaccaacgc cgaatgcccg
      661 tacgtcggca agcactacgg cgccgacaac atgcagaagc tgcagcgcga ggcaaccgcc
      721 gccggcgtcg tctggctgtc ggtgatctcc tcggcgcccg gcgaacaggg ctacgccgat
      781 gcgaaggaag ccgagcgcct gaacgccgag cgcaaggccg cgccgagcgc cgtgctgctc
      841 gatccggacg gccggctcgg ccatctgtac gacgcgcgcg tgacgccgca catgttcgtg
      901 atcggcccgg acggcacgct gcgctacatg ggcggcatcg acagcatccc gtcggcgcgt
      961 gccgccgaca tcgacaaggc cgagccctac ttccgcaacg ccctgcatca ggtgctcgat
     1021 ggccagccgg tcgagaaggc cgtgacccgg ccctacggct gcacggtcaa gtacgcgagc
     1081 tgacagagca gcgatgcgcg cgccgcgctg gccggcgcgc gcggccggcg gctaccgtcg
     1141 cgggctctgc aaagcagagc tgcccggagc cgcccgccga tgatcgattc ccccgccttc
     1201 tggctcgccg ccgtgccggc ggtgctgatc gtcggcctgt ccaagggcgg cttcggcggc
     1261 ggactcggcg tgctcggcgt gccgctgctg tcgctggcgg tgtcgccggt gcaggcggcg
     1321 gccgtgctgc tgccggtgct gtgcgtgatg gatctggcaa gcctctgggc cttccggcac
     1381 gtgccggtcg cccggcagct gcgcaagctg ctgccggggg cgctgctcgg catcctcgcc
     1441 ggcgcctggg cctggcagca cgttgacgtt gcctgggtga agctgatcgt cggcatcgag
     1501 gcgctggcct tcaccgcgca tcacgtctgg cagacgcgcg gcggccgcca gccgccggcg
     1561 cgtgccgtgt cggtgacgcg tgcgttcggc tggggggcgc tcgccggctt cacgagctat
     1621 ctcgcccacg ccggcggccc gccgctcgcc gcctgcctgc tgccgcagcg cctgccgcgc
     1681 gccgagttca tcggcgtgac cgtgttcttc ttcgcggtgg tcaattacac caagctcgtg
     1741 ccctacgcgc tgctcggcca gctcggcgac gccaacctcg tcaccgccgc ggcgctgctg
     1801 ccgctggccc tcgtcggcgt gcgcctcggc gtcgtgctgc atgcgcggct gccggaaaag
     1861 ccgttctacg cgatctgcta cgccgggctg gcgctgaccg gcacgaagct gctgtgggat
     1921 gcgctggcgg cgctgggcgg cggctgagga caccgccgcc cgcatcggga atccccggcg
     1981 ccccggcgac ctcaggaccg tcatggccct gccgccgccc cgcgcctgcg cacccgcagc
     2041 gcccgggcat cctgctcccg tccgcactcc ggccccggtc cgctgaaccg ccctgccggc
     2101 cggcgactct gctgcctgtg cctgaccacg ggaacaccag aggagacccg acatgaacct
     2161 gcgcgtgctg atgctcgtga cttcgcatga ccgcctcggc gacagcggtc acccgaccgg
     2221 cgtctgggcc gaggaactgg ccgtgcccta ctacgccttc accgatgccg gctgccacgt
     2281 cgacctcgcc tcgccggagg gcgggccggt gccgttcgat ccgcgcagcc tcgagaccgc
     2341 cgacggcccg ctgcccgcgg ccgtcgaacg cctgcaggcc gatgaggcgc tccgggagaa
     2401 gctccgccag agctggcgcg ccgctgccat cgacatgcac gtgttcgatg cgctgttcgt
     2461 gcccggcggt cacggcgcga tgtgggacct cgccaccgac ccggcggtcg cgcatctggt
     2521 cgaaacggcg tttgcgtccg ggcgcatcgt cgccgcgctc tgccacggac cggcggccct
     2581 ggtctcggcc aagggcccgg acggacgctt cctgctcgaa ggcaagcacg tcaacagctt
     2641 caccgacgcc gaggaagacg cgctcggcct cggcgacacc gtgcccttcc tgctggaaaa
     2701 ccggctgcgc gaactcggcg ccttcttcga aggcggtccg aactggcagc cgtacgcggt
     2761 gcgcgacggc aacctgatca ccggccagaa cccgcagtcc tcggcgctgg tcgcgcagca
     2821 ggtgctcgat gccctgcgtg cggggctgcc cgctccggcc tgagccgcac cgaggcatcc
     2881 gcaggaaatg tccgcgccgc ccgccccggc ggcacggcgc ctgccgccaa cccgtgtccg
     2941 caagcgcccc ggcggctgcg gaaggctccg gtccgcgccc ctgtcagcgc gcgtgcggga
     3001 acatcctgag ggccgaaacc agcaggacga tgccgagcag ccccgtcagc cagtcggtcg
     3061 gcacccgccc gagcagcagg gctcccacgg ccgcgcccag gatcgagccg agcaccatcg
     3121 agcccatcag cggcatttcc tgacgcaaca ccgagaaggc ctcggcgcgg gtgtagcggg
     3181 cgaagccgac catcatggtc ggcaggctga ccatcagcga caggctgccc gcgagcctga
     3241 tgtcgaggcc gtagagcagc acgatgaccg gaatcagcag ctcgccccct gccaccccca
     3301 gcagcgccgc gacgacgccg atcgcgaagc ctgcgaacac cccgctcacg aggcgcagag
     3361 ggccgggttc gagcagcggt gtgccggctt cgtgcaggcc ggcccgggtt tccagcagca
     3421 tgagcaggga cagcccgatc aggagcacca ggatgatgcg atccagccag cgccggggca
     3481 gcgtgacggc ctggcctgcc gcgcaccagg caccggcgag actgccggcg agcaggttca
     3541 gcatcacgtc gagatgatcg ccgagcgcgg ccggtgcgat cgtgacgccc cggaacagca
     3601 gcgcggcggt cacgacgacc aggctcatgg ccttgttgag gatcacggcc tccagcgtcc
     3661 ccagccggaa cacgccgatc agcaccggca gccggaattc ggccccgccg aggccgatca
     3721 gtccgcccag ggtgccgatc agggaaccgg cgacgaagcc ctgacggttg cggctccggg
     3781 cgttcaccgg cacgcccgct gacggacggc tgcatgccgc aggaccggta ccggccgctc
     3841 gtttttgtcg ccatccggga aattcatctg ctgcctgctg cccatgccgt cattgcaccg
     3901 atgcaccgtt gcggatgcgc tgatccggtc tgcgacttct gtcgggtcaa aaatgacatg
     3961 acggaccgcc cgaggcatcc cccgtgccgg aacatcagcc ggaattccgg ccggaagctc
     4021 acgaggatca ggcagccaga ggccaccgga gccggcttga gaagcgcttt cagccccgcg
     4081 ccggctgcgt gtgatcggca tcgcgcaccg gagcctgccg gaaacggctc ccggacgccg
     4141 ccggccccgc cgccacatag actggcctcc gccccaccgc cgccggagac cgccgtcgat
     4201 gagcttcgcg cagctgctgc agcgctactt ctacagccac tacgtgcaca gcggcctgcg
     4261 cgtcgcgctc ggcgtgctcg ggctgacgct cgcggcctgg gcgacgctcg ggccgcacgc
     4321 ggcggcggcg gtgtcctcgg gagccctcgg gacgagcctg accgacctgc ccgggccgct
     4381 ggccgagaag cgccggctca tgccggcggc ggtcgtgctc ggcacgctgg ccacactcgg
     4441 cgtgggcctc agtgccggta cgccgtggct caacgccgcg gtcgtgctcg gcatcacctt
     4501 cgtctcggcg ctgctgctcg gctggggccg ggcggcgctg ccgctcagcg tcggcacgct
     4561 gttcgtcacc gcgatggcgc tcggcagcca cgaggcggcg gccgatccct ggcagcacgc
     4621 cggctggttc ctggccggag ccggcggtta cctgctctgg ggcctggtcg ccgcgcgcgc
     4681 ggcggcgcgg cgcacgcgcc agcaggcgct ggccgagagc ctgcacgagc ttgccgaata
     4741 cctgcgcgcc aaggccgact gctacgtgcc gggggcggat ctcgaacgct gctacgcgca
     4801 gctcgtgcac cgccagacgc tgctggtcga gcgccagcag gcggcgcgcg atctggtgct
     4861 ggaggagctg cgcacgccgg ccgacggcac gctgctcgcg acgctgctcg cggccttcga
     4921 catctacgag gcggtgctct cggcgcacac cgaccacgcg ctgctgcacc gcacgctggc
     4981 cggggccgat gcgctcgact tcctgcacga actggtgctg aaggcggcgc gcgcgctcga
     5041 tacggtcgcc gcggcgctga tgcgcgacca gccgccaccg gcggcgaccg cctgcttcaa
     5101 gcccgagctc tacgcgctcg cctacgagcg cctgcgcctc gatgcgctgg cgccgacgcc
     5161 ggacaaccag gcggcgcgcg cggcgctggc ggcgaccgtc gccaagatcg agcgctgcgt
     5221 cgtgcgcatc caggaactgc cggccatcgc cagccggccg gtgccgccgg cggagatctt
     5281 cggcggcgcc gatttcgcgg ccttccgcag cccgccgctc gccgggctga agctgctgat
     5341 cgcgcagatc cgcgcgccgc gctcgccgct gctgcgccac gccctgcgcc tgagcgcggc
     5401 ggtcggggcg gcgctggcgc tcgggcgggt gctgccctat gaggcgcacg gctactggat
     5461 cgtgctgacc atcgcgctgg tgctgcgccc gaacttcagc atcacgcagc agcgccagaa
     5521 ggaccgcctg ctcggcaacc tgaccggctg cgtgctggcc gcggcgctgc tggcggccga
     5581 gccgccggcc gtgctgctgc tcgcggcgat cttcgtcgcc aacaccgtcg cgcacgcctt
     5641 cgtgacgctg cgctaccgct acacggccac cgccggctgc gtgatcgtgc tgctgcaggt
     5701 gcacctgacc ggcagcgggg ccggcgaggt gacgatgctc gcgcgcgtgc tcgacaccgt
     5761 gctcggcgga ctgctgtcga tcggtgcaag ccacctgctg ccgagctggg agcgctacgc
     5821 gatgccggcg ctgctgcgcg gcctgctcga tgccgccggc gcacacgcac gcgccacgct
     5881 cgccgagccg atggacgaac ttgcctaccg ccttgcgcgc aagcgctacc acgacgccgt
     5941 cgtcgcgctc gccggggcct tcgagcgcat gctcgccgag ccgcccgacc agcagcgcgc
     6001 ggtcgcggcg ctggagcgct acgtcgcgct cggctacctg ctcggcacgc agctcgtggc
     6061 gctgcacatg ctgcgcctgc accgcggcag cgagttcgag gccgacgcgc ccgaacgcct
     6121 gcgcgccagt gcggccgcgg tcgccgccag cctcgccgcc gccgcacacg cgatcgaccc
     6181 ggccgccgtc gcccccgaaa ccctgccacc ctcggcgaac gcgctcgccg acacgctcgc
     6241 cgatgacgcg ctgcccggcg actggcacgc cgaacgcctg ctggtgcgcc ggctggccct
     6301 gatccgcgcc agcgccgggg agctgcgcga gcgcgcgggt gcgctggcgg ccgcgggctg
     6361 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgccggcg ccgtccgcgc
     6481 gccgtgcctc cgcaccgctc cggcgctgcc ctcgccgagg tcactgctcc tgccacggcc
     6541 ggcgccggat ttcccgtagc gtgctcgctc ggcaagtaca gccccgtcgg caacctgatg
     6601 ggctcgctgg tcggcggcaa cctgtggctg gagggcgggt tcgcgcgaat gatgtatctg
     6661 tcgctgcaca agctgcacaa gctgcacaag ctggcgctgc acggcttctg gaaggtcgcg
     6721 ctcgatacgc tcgcccgcat gatcacgcgc cgcaccgaac cgcacgtgaa gctgcactga
     6781 gcaccgcgca ccgctgcctg aaacgacacc gcccggcaga agccgggcgg tgcggggggg
     6841 cgtacagcgg acagcgtgcg atcagacgcg cacgaggccc ttgtactcgg cgccgaagcc
     6901 ggtcagcagc tcgaccagcg ccttcgcatc ggcctggacc ttcgcgccga catccttcgc
     6961 gacgccgacc gaggcttcgc cgtaggcctt caggccggcc agatcgctga tctcggtggc
     7021 ggccttcaga cgcgacaggc tgagctcggc gtagtcctgc accagagcga actggaacga
     7081 ggtcagcttt tcgagctggg cgaccgacag cttgccggcg gcaacgatcg gggcggtgac
     7141 ggcttcgagg gacgggacgg agaacttggt catggcggct tcctcaaggt gcaggcgggg
     7201 tgttgtggga ttcgtctgcc gaatccatga gcccattata ggtatttcat gctgcagtgc
     7261 aacatgaatt tccacccacc ctgtttgcct tgattccatc gggctgcagc cgggtccgga
     7321 catgtctgcg accgctcttg cccggacgcc gcagcccggc gcaaccggcg ggtctgtgcg
     7381 cggtcgtgcc cccgctgatg cccggtgatg cggctcaggc tgcgaggcca aatcgttgac
     7441 gttcgtcaat ggcaatgatt gtcatttggc aatgcaggac gatacagtgc ggccgtaccc
     7501 gccctttccc gatccccagg agttcacccg atgaaggctc cgttgcgccc cgtgctggcc
     7561 gctgtcgccc tgctcgccct gcccgcgctt gccgtcgcca aggaatatcc ggtcggcaag
     7621 ccggtcgtga aaaacggcat ggagatcgcc gccgtctacc tgcagccggt gcgcatggag
     7681 ccggacggca tgatgcgcaa ggccgaggac tccgatgtcc acctcgaagc cgacatcaag
     7741 gccgcgaaga acaacccgaa cggtttcgcc gaaggcgact ggatgcccta cctgtcggtg
     7801 cagtacgagc tgagcaagca gggcggcgag acgcagtccg gcgagttcat gccgatggtc
     7861 gcctccgacg gcccgcacta cggcgacaac gtcaagctgc agggcccggg caagtacacg
     7921 ctgacgctga ccatcagccc gccgggcgcc aacccgcacg cgcacttcgg ccgccacacc
     7981 gacaaggaga ccggcgtcgg cccgtggttc gagcccttca aggtcagcta cgacttcgcc
     8041 ttcgccggca tcggcaagaa gggcggctac tgagccgccc gcgcccgcag gagcgccgtc
     8101 ccatggtccg tccgtccttc ctcctccgtc tgcatcccgg cctgcgcgcc gtcctcggcg
     8161 ccgcgctgct cgcggcggcc ggagcggcgt ccgcggccga tgcccacacc gtcacgctga
     8221 tcgcgaagga cggccgcttc gagcctgcga cgctcgaagc gccggccggc acgcgcatcc
     8281 gcatcgagat cgagaacgcc ggcgacacgc cgatcgagtt cgagagcacg ccgctgcgca
     8341 aggagaaggt gctcgcaccc ggcgcgcgct cggtggtcgt gatccagccg ctcgacccgg
     8401 gccgctaccc gttcttcgac gagttccacc ccgacaccgg ccgcggtgaa ctcgtcgtgc
     8461 gctgacttct ttcccgcctg cccgtcttgc ccggacctgc cgccatgaat gccgctgcct
     8521 gccctaccgg cgcgtcgccg cttgcgaccg ccgtccccgc ctcgcgcctc gcacgcatcg
     8581 gcctggcgct gcgccggcac ggacgcacga tccggatgat ccagtgggcg gtggtgttcg
     8641 tgtatctggc gctgctcttg atcccggcgg ccctgccgct gccgcccgag gacgcgcggc
     8701 tgttcgggca tctgggcacg atcgcgcagt tcgtgttctg gggcatctgg tggccgttcg
     8761 tgctgatcag catggtgctg ttcggccggg tctggtgcgg cgtgttctgc cccgagggcg
     8821 ccctcgccga gcaggcgagc cgcgtcgggc tcgggcgcgg catcccgcgc tggctgcgct
     8881 ggcagggctg gccgttcgtc gccttcgtgc tgacgacggt ctacgggcag ctggtcagcg
     8941 tctaccagta cccgctggcc gcggcgctgg tgctcggcgg ctcgacggtc gcggcgatcg
     9001 cgatcggcct gctgtacggg cgcaacaagc gcgtctggtg ccggcatctg tgcccggtga
     9061 gcggcgtgtt ctcgctgctc gcacggctgg cgccggtgca ctaccgcagt gaccccgaac
     9121 gctggacggc gaaggccggc gggccggtgc agccggtcaa ctgcgcgccg ctgctcgaca
     9181 tccgccgcct gcacggtgct gcccagtgcc atgcctgcgg gcgctgcgcc ggccagcgcg
     9241 gcgcggtcga gctgacggcg cgcgcaccgt ccgaggagat cgtccgccac ggtgcgcagg
     9301 atgcgacgcg ctgggatctg cgcctggtgc tgtacgggct ctgcggcgtg gcgatgggcg
     9361 ccttccactg gaccgcgagc ccgtggttca tcgacgccaa gcaggccgtg gcgatgtggc
     9421 tgctcgatca cggcggcgag cgcctgctcg acaccgatgc gccgtggtgg ctgctgacgc
     9481 accagcccga gcgccacgac gtgttctcgt ggctcgacgg cctgatgatc attgcctaca
     9541 tcggcgccac cggcctcgtg gtcggcgccg tgctgcatct gctgtgcgcc atcggcgcgc
     9601 ggctgctggc gcagcgcccg gcgccgaccg cgcacctgtc gctcgccttc gtgccgctcg
     9661 ccggggccgg cgtgttcctc ggcctgtcgg cgctgaccgt gtcgctgctg cgcggcgacg
     9721 gcttcgcgct gtactgggtc aacccgctgc gcgagctgct gctcgccggc gcggcgctct
     9781 ggacgctgcg cctcggctgg ggcatcagcg gccgctacgg cgccggcctg catcgccggc
     9841 tcggggcgac gctgctggtc gcggccgggg ccggcttcgt ggtctggggc tgggcgctgc
     9901 tgttctggct gtggtgaggg cgctggcgcc gcgctcctga accgcggccc gtgccgccgg
     9961 aaacgaaaac gccgccttgc gcaaaggcgg cgttttcacc ggtccggcga cggatgttca
    10021 gtgcagcagc atcagcccgg ccgcggcgct gagcgcgtgg tcgaagtgct cgatctcgcg
    10081 catgcgctcg cgcagttcct tgcggaagca gcgtgcggcg ctcgccagca gctcgatcag
    10141 ccgcggcagc gccggaccga aatccccggc gcgcgcgagc agcaactcgg cgctgaccca
    10201 gacctcctgg cgacgacggc tgaacttggc gagccggaaa cggcggttga ggtcggccat
    10261 cagatcgcag gcgacgcgct gcatcgtcag cgtgcccgag cagtggaagt tctggatgaa
    10321 caccagcagg aactccggat cctcctcatc gatgctgatg ccgatcgcaa agccgtcgtg
    10381 atggaagacg aggtcgccgt cgtcgtcgta gcgcggacgg tagccggcct gcacgagctg
    10441 atcgaagtag gcgttcaggc ggtcagacat cggcacgagc atgggagcgg ctccggacag
    10501 cgacagcggc gagacgttcg catattacgt acagttaata ttgattgcgc aagtcccgca
    10561 gacagtccgt aaccccgtgc aacgattgac gatttgctgc cacccgccat acgtcctgcc
    10621 ggtggtggct tctgacggga ccgacccggc cccgcccggc gcccgcgctg ccggacgggg
    10681 accggcccgg tgcttcaggc cgagaagccg ccgtcgatca tcaggttcgc gccggtgatg
    10741 tagccggcct cgggaccggc gagataggcg acgaagctcg cgatctcctc ggcacggccg
    10801 tagcggccgg tcgcgagcat cggcagcagc cccgccgcca tcgggccgtc ggccggattc
    10861 atctcggtgt cgaccgggcc gggctggacg gtgttgacgg tgatgccgcg cgcgccgaga
    10921 tcgcgcgcca tgcccttggt cagcccggcg accgcggcct tgctcatcgc gtacacggca
    10981 ccgccggcaa acggcatgcg ctcggcgttg gtgctgccga tcgtgatgat gcggccgccg
    11041 gcgccgaggt gacgcgcggc gatgcggctg gcgatgacga cggcgcgcac gttgaccgcg
    11101 agcgtgcggt cgagcccgtc gagcgggaag tcctcgatcg aaccgagcgc gagcacgccg
    11161 gcgctgttga ccaggatgtc gagccgtccg aactgcgcga ccgtgcgctc gatcgcggcc
    11221 tcgagggcgg catcgtcggc gctgtcggca cgcagcgcca gcgcccggcc gccggccgcc
    11281 tcgatcgtcc ctaccgtcgc cgcagcctcg gcctcggaac gcgcccaggt cagcgccacg
    11341 gcggcgccat cggcggcgag gcgctgcgcg atcgcagcac cgatgccgcg cgcgccgccg
    11401 gtcacgaagg cgaccttgcc ggccagcggg cgggcagcgg ggaccggggc ggcactggcg
    11461 gcattgctgg tgctggtcgt ggtttcagga ttcatgtcgg actccgctct gctgggggtg
    11521 gctgggaacg gggaccagca tgcgcgtccg attagcgact gattagtcgg caaaaccgcg
    11581 gttcagtcga taccggtggt ttacgctgga accctgactt cgcagccgcg gacacgacga
    11641 tggatacgct gaacggcctc gaatgcttcg tgcgcagcgc cgaggcccgc agctttgcgg
    11701 cggccggccg ccggctcggg ctgacgccgg ccgcggtcgg caagaacgtc gcgcgcctcg
    11761 aagccacgct cggcgtgcag ctgttccggc gcagcacgcg gcggctgacg ctgaccgagg
    11821 ccggcgagga tctgctgcac gccgcggccg gcccgctgga ggccctgaac ggcgcactcg
    11881 cgcagctcgc cggcagccgc tcgacgccct ccggcacgct gaaggtcggt cttgcaccgg
    11941 ctttcgggcg cgactacatc ctgccgctgc tcggcggctt tctcgcccgt tacccgctga
    12001 tccgcccgga ctggcgcttc gagaaccggc ccgtcgatct gatcggcgag ggctacgacg
    12061 cggcgatcgg cggcggcttc gaactgccgc cgggcgccat cgcccgcgaa ctggcgccgg
    12121 cgcaccgcgt gctgctggcc gccccggcct atctggccac gcacccggca ccgcagcatc
    12181 cggccgaact gccctgccac accggcatcc tgatccgctc gctgcagacc gggcgcttcc
    12241 cggcctggac gctgcgcgac agccacggcg cgcaggcccc ggtcgggctg ccggtcagca
    12301 tcagcgtcga tgaccccgag gccgcctgcc acgcggcgcg tgccgggctc ggcatcacgc
    12361 tggcgagcac cgcccacgcc ctgcccttcc tcgaagccgg cacgctgctg cgcgtgctgc
    12421 ccgactggca cgtcgacgcc ggacgcatcg cgatctactt cccggcccag cgcctgctgg
    12481 ccccgaagac ccgcgccttc gtcgattacg tcgtcgaaca gtgtcgcgcg cagcggctgg
    12541 ccgagcggct gtcggcggtg taagcgcggc cggcatcggg atagcgtcgc gcggctggag
    12601 gcggttgccg gtcacgggcc gatcaggtcg ggggtgcccg ggagaccggc atttccctga
    12661 gcctcagctc gcgctgactt gaggcgcgac gctatcccga cgcccgcggc gcacgccgga
    12721 gcccgccgct gcggcaggcg tcccgaaagc ctcgtttcga cgaacctgcc cgctccggta
    12781 caggaaaatt cctttttgca agattagaat ctcggcaacc ataaaaatct ataaacaggt
    12841 agccaccgtg gtcgatccgg ttcaacgcat caacgcgctg caggagcgcg ctgcggtcac
    12901 gaaacccccg gcgctgccgc gctgggagga ctggcagcgc agcgtgccga acgaggtcgt
    12961 gcgctcggcg ctgttcacgg tcggcaaccg gcgcgaccgg cgcgagtttc tggtcgagac
    13021 cgaactggcc gccatcggcg agacgcaagt cacctaccag ggcgtcgaac tgcgccagga
    13081 cgatgaggat gtctggctgc aggcgctgca cctggcgcgc gaacaaccgc tcgatgcgcc
    13141 gatcgagttc gtcccgcatg ccttcctgcg cgcgatcggc tggcccacca ccgggcacta
    13201 ctacgcgctg ctgcgcgatg cgttcggccg catgcagcgc acgacggtca ccttccgcaa
    13261 ccagcgcctg cacaagggcc tgtcgatctc gctgatccgg cgcttcgagt ggagcgaccg
    13321 cgacgccggt ggcagtgacg atcgctgcaa ggtctggatc gagccggaga tgcggctgct
    13381 gttcggcaac acgcagtaca cgcgcatcga atgggaacag cgccggcacc tgcgaccgct
    13441 cgcgaagtgg ctgcatggct acttcgcgag ccatcgcacg ccgctgccgg tgcgggtcgt
    13501 gacgctgcgt gatgcctgcg gttcggacac ccgcgagctg cgcaacttcc gcccgaagct
    13561 caaggccgcg ctgaccgagc tggtcgaagc ctgcttcctg agcgactggc agatcgacga
    13621 gaccgacctc gtgcacgtgt gccgggcacc ggcacccggc gtggacgcct gtggacaaac
    13681 gcgcctcatc tgacgaaccg gcattcgtaa catgaggcgc agagattcgt aacatgaggc
    13741 gcacccacac gtaatatgag gcgcaggcac gtaacatgag gcgcaggagg cacgtaacat
    13801 gaggcgcaaa acacacgtaa catgaggcgc ggcagctcaa aaaatgcgct cgcaagcaat
    13861 tgaataaaaa ggcgaatttc gtttgtatca aaaacactaa ctattctttt aaccattctt
    13921 ttaaccaaaa gaaaaagagc gcctccgatg tcgaaaccga ccggccccgc ccccgactac
    13981 tcgctgacga ccgatcggct gcgtgcgctc ggcgaggtca gcagcaagct gatccacgaa
    14041 ctgcgcgcct actcgaccag cccggaacag cgcaagctgc tgcagaagca gtggcgcatc
    14101 ggggaagtcg ccaaactgct cggcatcagt gaatccagcc tgcgcaacca cgacgaagcg
    14161 ctgcgcggtg gcgaaggcaa caccggaggc gcgcaccggc gctactcgct gaacgacatc
    14221 cacagactgc gcgaactgac ccaccgccag gcacaccggg acccgctgcg cgatcgctgt
    14281 gcccgactcg ccgtctgcaa cttcaagggc ggcagcggca agagcacgac ggcaaccaac
    14341 ctcgccacgc atctggtcct gcacggttac cgcacgctgt tcatcgacat ggacccgcag
    14401 ggctcggcga ccgccctgct gtacccggac aacctgatgg acatccagcg cgagaacacc
    14461 ctctacaagg tgctgttcac gcagcccgaa cgcatccgcg atctcgtgat ccagaccgcc
    14521 tggcaaggac tggacatgat tccggccgcc ctgtcgctgt tctcggtcga agccgagatc
    14581 ctgcgctacg gcttcagcaa cccggacttc ctgttctggg aacgccttga tcgggccctg
    14641 ggacacctgc aggacgacta cgacgtaatc ctgttcgaca ccccgccgtc gctgtcgcag
    14701 accaccgtga tcgccgcctg gagcgccacg gccctggtga tccccgtgca ggcctcgatg
    14761 atggacctgc aggcgatggc ctcgttcttc ttcggcatgt ggcagtcgct gtcggccatc
    14821 gaacagatca ccggcgagac caagcactac gacttcggac tgatcgtgat cagcaactac
    14881 cgcggcaatc cgaagacgat gcaggacgag tacgacgtcg ccacctggat gcgaaaaatg
    14941 tatcagcact acgttgccga ggacgcgatc ctgaactcca gcgtcatccg ttcagccgcc
    15001 aacagcatcc agatcttcta cgagctgccg aggcgctcgg atccctccta tgcccgcgca
    15061 ctcggcgcct tcgaaggtct tggcgaccag atcatcggcc tgatccgccg gcagtggcca
    15121 tcgacgcaga cggcagccac gcgcacggcc agcgcttcct gacccggaac ccgtcatgac
    15181 cagcaagccg acccgcagga aaccgcgctt cgaaggcctg accctgccaa gcatcctcga
    15241 cgcgactcca gccgcgccgg gaactgcacc ggccccggcc gtgccggttg ccgactacgt
    15301 gcgcgaggcc ggccagcggc tgaccgagga ctaccgtcgc ctgcgccagc tcgaacagag
    15361 cggacgagtc gagctcgaac tgccgctggc gagcatccgg ccatcgcgct tcgccaaccg
    15421 cagcgagcac tacttccaca gcgcgcgcta tgccgaaatc cgcagcagca tcgcgcagca
    15481 cggcgtgaag attccgatca aggtccggcc ggtcgctgac agcacaccgc cggcctacga
    15541 aatcgtttac ggccacacgc gccatcgcgc cagcctcgat ctcggcctgc cgagcatccg
    15601 tgccgtcgtc gaagcgctcg acgacgaaac cctgctcgcc gagatggaac tcgagaaccg
    15661 cgaccgcgag gatctctctg cctacgagcg tgcccgcttc tacgcccttg cccttgccga
    15721 cacctttgcc ggcaaccgcg aacggctgtg tgcccgcttc gggcgcagca atgcctggct
    15781 gtcgcagcag cgcgtgatcg ccggcctgcc acagcgtctg ttcgacgcga tgccgctgct
    15841 gtaccaggcc ggtgtcgagc cgctgcttga actcgcacgg ctgcgcccga ccgatgaaca
    15901 ggtgcagacg ctgatcgaga tgcacccgga tgcgtccagt gccaccaccc tcgtgcaggt
    15961 cctgatacgc cggctgcact ccgcaagccg cggcagcccg aagcgcacga cggccagcgc
    16021 tgaagccgtg catcaccatc gcaacggccg cctgctgatg cacgcacggc ctggcacacg
    16081 tggtcgcgtg cagttcgagt tcgaatccgg ccagcgtggt ttcgccgatt tcctgcgcac
    16141 ccggctcgct gagctgtatg a
//
LOCUS       DAOQIE010000043        16103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1705_length_16103_cov_7.566052, whole genome shotgun sequence.
ACCESSION   DAOQIE010000043 DAOQIE010000000
VERSION     DAOQIE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16103
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_16103_cov_7.566052"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK144_04390"
     CDS             <1..456
                     /locus_tag="PK144_04390"
                     /inference="COORDINATES: protein motif:HMM:NF020680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HNF64999.1"
                     /translation="WLAPRCGEADAALLLALAAGSPLRALALAGALERRRELFRAFLD
                     LAAGKLDAVRAAERFLAGGAPAEVAGWLAGWHHDLLRLTMAAGSAPLTNPDYAAPLAT
                     LAARVAPQWLVLRWDAVLALRARLATPLNAQLAFEDFFIDWAGAAARRG"
     gene            467..835
                     /locus_tag="PK144_04395"
     CDS             467..835
                     /locus_tag="PK144_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNF65000.1"
                     /translation="MEPTRGNAAAASKQGVMSIALRDRNQLLQCFMPFLKHGGLFIPT
                     DKPFELGDDVFLLLTLMDEPTRYPVTGKVVWLTPKTAIGGRPAGIGVQFAGMEAPTVL
                     KKIETYLAGILTSDRTTYTL"
     gene            882..1661
                     /locus_tag="PK144_04400"
     CDS             882..1661
                     /locus_tag="PK144_04400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNF65001.1"
                     /translation="MLVDSHCHLDRVDLRPFGGELAGVLDAAAAAGVGHLLCVGIALE
                     TWPAMVEQVAPFANVSLSVGVHPSEDGREPQVAELVRLARTTARVVAIGETGLDYHYG
                     AERGDLQRAWFRTHVRAARETGKPLIVHTREARADTLAILAEEGAAEVGGVLHCFTED
                     WDMAARAVDLNFHVSFSGIVTFKSAAQIQEVARRLPAERMLVETDSPYLAPVPHRGRS
                     NHPAWVREVAAFVAKLRGEPFEQLAATTTGNFFRLFRDAVH"
     gene            1754..2401
                     /locus_tag="PK144_04405"
     CDS             1754..2401
                     /locus_tag="PK144_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNF65002.1"
                     /translation="MTADTNGSTNAGISGSGLLARIRTRLALAPDPASLGGDHAVAGL
                     PRPERVLTPAAVLVPIVLRADGPSVLLTQRTEHLLNHPGQISFPGGRSEAEDAGPVDT
                     ALREAEEEIGLARRHVEVVGFLDLYQTVTGFLVTPVVAFVEPGFALSLDAFEVAEAFE
                     VPLAFVLDAGNHTRGSRLVDGRERQFYVLPYQDRYIWGATAAMLVNLAHKLAPDD"
     gene            complement(2415..3374)
                     /locus_tag="PK144_04410"
     CDS             complement(2415..3374)
                     /locus_tag="PK144_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNF65003.1"
                     /translation="MSEPDPIRRRLLAAVAALPVLLAPLPAAAAGSSKPQAARGAAPR
                     RSARPAPSARPPRTPAPLLNTLVGARIEALRGRGLLDRSETVAFYVQDLLSGRVLLSI
                     NGSAAMQAASMVKPYVAAAFFAEVQAGRLRYDRSQQALMEAMIVHSDNAATNHFIRAL
                     GGPARVVAALRRVAPGIASGANINEYIPAGGRTYRNRIACTTHARFLGELWSLRLPGA
                     RELLRVMGLPNRDRICSGAPGIPPGTGIHDKTGTTALLCGDMGIVVARTQAGGQWPYA
                     FIGIVQRRTPSASYGRFMHTRGDAIRDISGLVYRELRPHYGLA"
     gene            3562..5154
                     /locus_tag="PK144_04415"
     CDS             3562..5154
                     /locus_tag="PK144_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNF65004.1"
                     /translation="MNDFLDEMNRRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGSV
                     KGRKAARHATSDWMELEKQRGISVTSSVMQFPYRGRIVNLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCAKGVEERTIKLMDVCRLRDTPILTFINKLDREGREPLELLDEVEKVL
                     KIRCAPVTWPIGMGRELKGVYHLLEDFVHLYQPSKDGRVNEGAIIPGLGSDEAAAALG
                     PEVIDALREEIELVQGAADPFDHGEYLAGRQTPVFFGSAISNFGVQEMLDGFVTHAPG
                     PRPRAADARTVAPHEPAFTGFVFKIQANMDPQHRDRIAFLRVCSGVWRAGMKAQHVRL
                     GREVKISDALTFMASEREHVDAAWPGDIIGLHNHGTIRIGDTFTEGEKLAYTGVPDFA
                     PELFRRARLRDPLKMKQLQKGLEQLCEEGATQLFKPLHSNDLILGAVGVLQFDVAAYR
                     LKSEYGVDCLFENVSVATARWVSCTDAKRFEEFRNKCADNLALDHHGELVYIAPTRVN
                     LQLTQERWPAVKFSATRDHAAR"
     gene            5347..5757
                     /locus_tag="PK144_04420"
     CDS             5347..5757
                     /locus_tag="PK144_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65005.1"
                     /translation="MNTPHACAGNPQIEPMNVWPLPATAWVEASVMDGEQSIAVIFDP
                     RPFLAMASESELALLARARWSGRSIMRAVQCLAHAGDAELRRWFRQFGDWLDAEHDIT
                     IDGSIDPQLALQWLGTHRPQVVPGVLAASRSWLH"
     gene            5888..6790
                     /locus_tag="PK144_04425"
     CDS             5888..6790
                     /locus_tag="PK144_04425"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF65006.1"
                     /translation="MNDLTGYAVGFIGLGLMGRPMALNLHCAGATVTVHNRSRAPVEA
                     LAAKGLRTADSAGTAAAAGDFVILMLTDTPAVDAVLEGPDGVLAGLRPGALVIDMGST
                     SPLATREFAARVAEAGGEWLDAPVSGGVVGAEAATLSIMAGGSIEALERARPLLERLG
                     ARLTHVGPSGCGQIAKTVNQMIVGLTIGAVAEALALAGRAGADPARVRAALVGGFADS
                     RILELHGLRMIDGDFTAGGRVSTQHKDLLQALELAGAVGLELPATELNRRLYAECIAA
                     GDGGLDHSALYRHYTRTATTGGEG"
     gene            6796..7023
                     /locus_tag="PK144_04430"
     CDS             6796..7023
                     /locus_tag="PK144_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HNF65007.1"
                     /translation="MSRDDETPTGRPLRLHELAASALWALLGVQKEANRRRDFARGRP
                     GQFVLIGVIATVLFVLLLIGLVRLALGLAGA"
     gene            complement(7026..7778)
                     /locus_tag="PK144_04435"
     CDS             complement(7026..7778)
                     /locus_tag="PK144_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNF65008.1"
                     /translation="MADFRTHLIVGTAASGIGAGALLSIGLLSAAHAPACFALGAIGA
                     LLPDVDLDHSIPARVAFSLVALAGAFALVLALAPRWSLAELTLAGAAAWVVLRHGVFG
                     TINRWTVHRGLVHSLPAAAAFALATVLAAQRLYGLPVEAAWLAGGFLGAGFVVHLLLD
                     EIYSVDLLGRRLKRSFGTALSFGDPRNLPGTLALYALAGGLYWLCPPAGGLGQRLLDA
                     GLYRQLGARLLPAHGWFEGLPGLALEGLRRLL"
     gene            complement(7876..8946)
                     /locus_tag="PK144_04440"
     CDS             complement(7876..8946)
                     /locus_tag="PK144_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HNF65009.1"
                     /translation="MSKTDSYDGKPRSLLSVLIHAYHPRTWRERGILYAAGVLLATYV
                     IIVGVLWLIWDSKPSLFEPREIALAMAGGDEKKLVAGYVTTATVIQVADTLLEKPGGY
                     LSNDRMPPGVFMDNVPNWEFGVLTAIRDISRSLRNDFSRAQTQSREDVDLQVAEPKFN
                     FDNNSWLLPPSESEYRDGIRALRRYLDRLADERRDDGQFFTRADNLRDYFSVVEKRLG
                     SLSQRLSASVGHIRVNTNLAGDPNARQSTPTPQEQLIKTSWLEIDDHFYEARGYCWAM
                     IEFLRAIEIDFKDVLANKNAQVSLRQIIHELEATQEPVWSPVILNGSGFALFANHSLV
                     MASYIARANAALIDLRNLLQQG"
     gene            complement(9144..11312)
                     /locus_tag="PK144_04445"
     CDS             complement(9144..11312)
                     /locus_tag="PK144_04445"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNF65010.1"
                     /translation="MTERVAIGGLQVAKPLYDLVQNTLLPGTGLDAAGWWTALEAIVT
                     DLAPRNRELLAKRDALQAQIDAWHVARRGQPHDHAAYVAFLREIGYLLPEGPDFQVST
                     DNVDPEIASVAGPQLVVPVNNARYALNAANARWGSLYDALYGTDVLPEDGGAEKGKGY
                     NPARGDKVIAYAAGFLDQAVPLASGKHAEVAEYLRDGATLVAQLADGTKTALADPSAF
                     VGYVETDGALKTFLLRHHGLHVEVQLDRKHPVGAQSRAGVKDVVLESALTTIQDCEDS
                     VAAVDGEDKALVYSNWFGLITGSLTAEFDKGGKSVERALNEDRAYTAPDGSTLTLPGR
                     ALLLVRNVGHLMTNPAVLFGAGAAEIPEGILDALVTATISYRDLKGLNRLKNSRRGSM
                     YIVKPKMHGPEEVAFAVELFGRVEDALGLARNSLKIGIMDEERRTTVNLKECIRAARE
                     RVIFINTGFLDRTGDEIHTSMEMGPVIRKGDIKNAAWIKAYEDWNVDIGLACGLRGRA
                     QIGKGMWAMPDEMKAMVATKQMHPLAGANTAWVPSPTAATLHAMHYHQVDVAARQAEL
                     ASRPRASLDAILTPPVTAEANWSAAELQQELDNNAQGILGYVVRWIDQGVGCSKVPDI
                     NDVGLMEDRATLRISSQHIASWLHHGVVGREQVMETFRRMALVVDRQNAGDPLYRPMA
                     PAYDGIAFQAALDLVFLGRETPNGYTEPHLHKRRRELKGS"
     gene            11708..13090
                     /locus_tag="PK144_04450"
     CDS             11708..13090
                     /locus_tag="PK144_04450"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNF65011.1"
                     /translation="MVVEAVLRTLDWLAIAMAGWLAFVLRFDAAVMPGPFQRAMIIAL
                     LVSIGVFGRCSVYRAWRGETLNGELRTLLWAWCLTFLITIGALALTTGRDEAPFPRLW
                     LMLWFVLGCCLLTLLRGCLRQVLRRLRSSGHNTRRVVVVVAGELGVSVIRRLCEARWT
                     GLRVVGYFDDRPIERVGEIAGVPRIGRIDELAAYVEGGHADQVWITLPFRAEERVREI
                     LETLRHATADIRMVPDILGLQLLNHSMGTVAGIPVINLSSTPMVGFNRVIKALEDRMI
                     SLAILLMIAPLMIAIAIGIKLSSPGPVFFRQERLGYDGRPFRVWKFRSMKVHQEHGGQ
                     VTQATRGDSRITPFGAFLRRTSLDELPQFFNVLDGSMSIVGPRPHAVAHNQEYKELVD
                     SYMRRHKVKPGITGLAQVRGYRGETDTVDKMKLRVESDLEYIENWSLGLDLKIILQTI
                     FKGFVNPNAY"
     gene            13151..14566
                     /locus_tag="PK144_04455"
     CDS             13151..14566
                     /locus_tag="PK144_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNF65012.1"
                     /translation="MKRALVAESSVMRGLLARSLDIATIVASSFAGWWLETGSTGWFE
                     GDFLRAALIGLLLTGICSAHWAVYRSWRGTPLRSELEAVLGAWLLAVLLLVPVLELTG
                     EPDEYGRHWLLYWALFGTAGLAGWRVLMRRAQRYVHTGGRDVRRVVLVTNGGFGLQII
                     EQLATARWAGFEFVGWFDERDGDDGRISAAAVQRCLGPTERLAGFVEDGNADEVWIAL
                     PFRAEARLRSILWSLRHCTADIRLVPDISSFRLLNHSLGEVAGLPVINLSSTPMSGRN
                     RLLKAIEDRTLAGACLILAAPLLLVIALGVYLTTPGPLFFRPVRLGYDGRPFRAWKFR
                     CAVPHAGRNRFTRFGVFLRHTGLEGLPQLLNVLDGSMAIVGPCPHAAAYNAQYKARVD
                     GYMSRFKVKPGLTGWAQIHGHDQRIESIEDMERRLALDLEYIENWSLELDLRIVLLTV
                     WQMAGRVLGGLRKAAAQQIRH"
     gene            complement(14563..14907)
                     /locus_tag="PK144_04460"
     CDS             complement(14563..14907)
                     /locus_tag="PK144_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HNF65013.1"
                     /translation="MSPVHRRRLLPVTALCVLLAAGSPPVLAEPLADALAAGRVGERP
                     DGLIGAVTPDAGARALVERVNAERMARYRDIAERNGRPLAEVQALAGRRLTAGAADGQ
                     YVMDAAGRWQRR"
     gene            complement(14904..15185)
                     /locus_tag="PK144_04465"
     CDS             complement(14904..15185)
                     /locus_tag="PK144_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HNF65014.1"
                     /translation="MILRNPVFPSSPCRHRPPIVAIVALLAIGAACAACTPTVKVEAP
                     DKPIEINLNVHIQQDVRVKLDRDLDTAFGDHPELFGPAAKARKPQESRP"
     gene            complement(15182..>16103)
                     /locus_tag="PK144_04470"
     CDS             complement(15182..>16103)
                     /locus_tag="PK144_04470"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HNF65015.1"
                     /translation="GELKLDLLHLRFGTAPPAVALAALSPALAAQLSVPGGGLRLQAR
                     ADWGGGAAARSGGELRIDGLELRFGGVRARGIDGVLRADRLWPLRIPAGQPLRIARLD
                     AGLPFEDGRVEFTLDDTQLRLRQAGFTLAGGRLELAPLRLDGPAPRPLAASARGLDLS
                     RLLAIVPVDGLGGTGTLNGSLAARLEADGLHDVQAQFASDGSGTLHYTPSTPPAALAD
                     GQAGFVLDVLRDFRYESLSLTLAGERAESLAAGLNVRGANPQAYGGHPVALNLNLSGA
                     LAGLLQQGLDSYRLPETVRRRLESSDRENP"
BASE COUNT         2313 a         5574 c         5774 g         2442 t
ORIGIN      
        1 tggctggcgc cgcgctgcgg cgaggccgat gcggcgctgc tgctcgcgct cgccgccggc
       61 agcccgctgc gggcgctggc gctcgccggg gcgctggagc ggcggcgcga gctgttccgt
      121 gccttcctcg atctggccgc cggcaagctc gatgcggtgc gcgccgccga gcgcttcctc
      181 gccggcggcg caccggccga ggtcgccggc tggctggccg gctggcacca tgatctgctg
      241 cgccttacca tggccgccgg cagcgcgccg ctgacgaacc ccgactacgc ggccccgctc
      301 gcgacgctgg ccgcccgcgt cgcgccgcag tggctggtcc tgcgctggga cgcggtgctc
      361 gcgctgcgcg cgcggcttgc cacgccgctg aacgcgcagc tcgcgttcga ggacttcttc
      421 atcgactggg ccggcgccgc cgcccgcagg ggctgacccg atgggaatgg aaccgacacg
      481 cggcaatgcc gcggccgcct ccaagcaggg cgtgatgtcg atcgcgctgc gcgaccgcaa
      541 ccagctgctg cagtgcttca tgccgttcct gaagcacggc ggcctgttca ttccgaccga
      601 caagcccttc gaactcggcg acgatgtctt cctgctgctg accctcatgg acgagccgac
      661 gcgctatccg gtgaccggca aggtcgtctg gctgacgccg aagaccgcga tcggcggccg
      721 gccggccggc atcggcgtgc agttcgccgg catggaggcg ccgaccgtgc tgaagaagat
      781 cgagacctac ctggccggca tcctgacttc ggaccgcacc acctacacgc tgtgatcgct
      841 gacgggcacc cgcggcccgg cagcggcgcc ggagaccgca catgctcgtc gattcccact
      901 gtcacctcga tcgcgtcgac ctgcgcccgt tcggcggcga actggccggc gtgctcgatg
      961 ccgccgctgc ggccggcgtc gggcacctgc tctgcgtcgg catcgccctg gagacctggc
     1021 cggcgatggt cgagcaggtc gcaccgtttg cgaacgtctc gctgtcggtc ggcgtacacc
     1081 cgagcgagga cgggcgcgag ccgcaggtcg ccgaactcgt gcggctggcg cgcaccacgg
     1141 cgcgcgtggt cgccatcggc gagaccggcc tcgactacca ctacggcgcc gaacgcggcg
     1201 atctgcagcg cgcgtggttc cgcacgcacg tgcgcgccgc ccgcgagacc ggcaagccgc
     1261 tgatcgtgca cacgcgcgag gcgcgcgccg acacgctggc gatcctcgcc gaagagggcg
     1321 cggccgaggt cggcggcgtg ctgcattgtt tcaccgagga ctgggacatg gcggcgcgcg
     1381 ccgtcgacct gaacttccat gtgtcgttct cgggcatcgt cacctttaaa agcgccgcgc
     1441 agattcagga agtcgcgcgc cggctgcccg ccgaacgcat gctggtcgag accgactcgc
     1501 cgtatctggc accggtgccg caccgcggcc gcagcaatca cccggcctgg gtgcgcgagg
     1561 tcgcggcctt cgtcgcaaag ctgcgcggcg aacccttcga gcagctggcg gcaacgacga
     1621 ccggcaattt tttcaggctg tttcgcgatg ccgtgcactg aacgcccggc gctgtcatga
     1681 agcggtaaac tccatgctcg ctgccgcggt tctgacgcgc attgacggag agccccgctt
     1741 catgattgcc gagatgaccg ctgacacgaa cggcagcacg aacgccggaa tttccggctc
     1801 cgggctgctc gcgcgcatcc gcacgcggct ggcgcttgcc cccgatccgg cgagcctcgg
     1861 cggcgaccac gcggtcgccg gcctgccgcg ccccgagcgc gtgctgacgc cggcggcggt
     1921 gctggtgccg atcgtgctgc gcgccgacgg gccgagcgtg ctgctgacgc agcgcaccga
     1981 gcacctgctg aaccaccccg gccagatcag cttcccgggc ggacgcagcg aagccgagga
     2041 tgccgggccg gtggacacgg cgctgcgcga ggccgaggaa gagatcggtc tggcgcgccg
     2101 gcacgtcgag gtggtcggct tcctcgacct gtaccagacc gtcaccggct ttctggtgac
     2161 gccggtcgtc gccttcgtcg agccgggctt cgcgctgagc ctcgacgcct tcgaggtcgc
     2221 cgaggctttc gaggtgccgc tcgccttcgt gctcgacgcc ggcaaccaca cgcgcggcag
     2281 ccgcctcgtc gatggccgcg agcggcagtt ctacgtgctg ccctaccagg atcgctacat
     2341 ctggggcgcg accgcggcga tgctcgtcaa cctcgcgcac aagctcgccc cggacgactg
     2401 atccggggcc ggcttcaggc cagcccgtag tgcgggcgca gctcgcggta gaccaggccc
     2461 gagatgtcgc ggatcgcgtc gccgcgcgtg tgcatgaagc gtccgtagct cgcactcggc
     2521 gtgcgccgct gcacgatgcc gatgaacgcg tacggccact ggccgccggc ctgcgtgcgc
     2581 gcgacgacga tgcccatgtc accgcagagc agcgccgtcg tgccggtctt gtcgtggatg
     2641 ccggtgccgg gcgggatgcc gggtgcgccg ctgcagatgc ggtcacgatt cggcaggccc
     2701 atcacgcgca gcagctcgcg cgcgcccggc agacgcaggc tccagagctc gccgaggaag
     2761 cgcgcgtgcg tggtgcaggc gatgcggttg cggtaggtgc ggccgccggc cgggatgtac
     2821 tcgttgatgt tcgcaccgct ggcgatgccg ggcgcgacgc ggcgcagcgc cgcgaccacg
     2881 cgcgccggcc cgccgagcgc gcggatgaag tgattggtcg cggcgttgtc gctgtgcacg
     2941 atcatcgctt ccatcagcgc ctgctgcgag cggtcgtagc gcagccgccc ggcctgcacc
     3001 tcggcgaaga acgcggcggc gacgtagggc ttgaccatgc tcgccgcctg catcgccgcc
     3061 gagccgttga tcgacagcag cacgcggccg gacagcaggt cctgcacgta aaaggcgacc
     3121 gtctcgctgc gatcgagcag gccgcggccg cgcaaggcct cgatgcgggc gccgaccagc
     3181 gtgttcagca gcggtgccgg cgtacgcggc ggccgggcgg acggtgcagg gcgggcgctg
     3241 cggcgtggcg cggcacctct tgcggcttgc ggcttgctgc tgccggcggc tgcggccggc
     3301 agcggggcga gcagcacggg cagggccgca acggccgcga gcaggcggcg gcggatcgga
     3361 tcaggctcgg acatggcggg acgggcgtgc ggcggaatga ccccggcagt atggccagat
     3421 gctccggcag cgtcaccccg gatgcggcgg tcgccggtgc cgggccggcg gggctgcggt
     3481 agactccgcg gtctttttcg gcgccggacg ggctgtccgc cggcatccat ggtccccgaa
     3541 cgacgcacag cgcagggcgc gatgaacgac ttcctcgacg agatgaaccg gcggcgcacc
     3601 ttcgcgatca tctcccaccc cgacgccggc aagaccacgc tgaccgagaa gctgctgctg
     3661 ttcggcggcg cgatccagct cgccggctcg gtcaagggcc gcaaggccgc ccgccacgcg
     3721 acctccgact ggatggagct cgaaaagcag cgcggcatct cggtgacctc gtcggtgatg
     3781 cagttcccgt atcgcgggcg catcgtcaac ctgctcgaca cccccggcca cgaggacttc
     3841 tccgaggaca cctaccggac gctgaccgcg gtcgactcgg cgctgatggt catcgactgc
     3901 gccaagggcg tcgaggagcg cacgatcaag ctgatggatg tctgccggct gcgcgacacg
     3961 ccgatcctga ccttcatcaa caagctcgac cgcgagggcc gcgagccgct cgaactgctc
     4021 gacgaggtcg agaaggtcct gaagatccgc tgcgcgccgg tgacctggcc gatcggcatg
     4081 ggccgcgaac tgaagggcgt ctatcacctg ctcgaagact tcgtgcacct gtaccagccc
     4141 tcgaaggacg gccgcgtcaa cgagggcgcg atcatcccgg gactcggttc cgacgaggcc
     4201 gccgccgcgc tcggccccga ggtcatcgac gcgctgcgcg aggagatcga actcgtgcag
     4261 ggcgcggccg atccgttcga tcacggcgaa tacctcgccg ggcggcagac gccggtgttc
     4321 ttcggctcgg cgatcagcaa cttcggcgtg caggagatgc tcgacggctt cgtcacccac
     4381 gcgccggggc cgcgcccgcg tgccgccgat gcgcgtacgg tcgcgccgca cgagccggcc
     4441 ttcaccggct tcgtgttcaa gatccaggcg aacatggacc cgcagcaccg cgaccgcatc
     4501 gccttcctgc gcgtgtgttc gggcgtctgg cgcgccggca tgaaggcgca gcacgtgcgc
     4561 ctcggccgcg aggtcaagat cagcgatgcg ctgaccttca tggccagcga gcgcgagcac
     4621 gtcgatgccg cctggccggg cgacatcatc ggcctgcaca accacggcac gatccgcatc
     4681 ggcgacacct tcaccgaggg cgagaagctc gcctacaccg gcgtgccgga cttcgcgccg
     4741 gagctgttcc ggcgcgcgcg cctgcgcgat ccgctgaaga tgaagcagct gcagaagggg
     4801 ctggagcagc tctgcgagga aggcgcgacg cagctgttca agccgctgca ctcgaacgac
     4861 ctgatcctcg gtgcggtcgg cgtgctgcag ttcgacgtgg ccgcctaccg gctgaagagc
     4921 gagtacggcg tcgactgcct gttcgagaac gtttcggtcg ccaccgcgcg ctgggtcagc
     4981 tgcaccgacg cgaagcgctt cgaggaattc cgcaacaagt gcgcggacaa cctcgcgctc
     5041 gatcaccacg gcgagctggt ctacatcgcg ccgacgcgcg tgaacctgca gctgacgcag
     5101 gaacgctggc cggcggtgaa gttctcggcg acgcgcgatc acgcggcgcg ctgatcccgc
     5161 agcccggctg cagcccgcct tgcaaattgc aaacgccgga gcccgtctcc ggcaggcgcg
     5221 gctgcagtct gcgcgcacgc agcggcaaaa ggcagggcgg cagcggctgc gtcgccggtg
     5281 gcatcgctct tgctcttcct gatcgcacgg caccggcaaa ccgggcaagg gagagagagc
     5341 gacatcatga acaccccgca cgcatgcgca ggcaatccgc agatcgaacc gatgaacgtc
     5401 tggccgctgc cggccaccgc ctgggtcgag gcgagcgtca tggacggcga gcagagcatc
     5461 gccgtcatct tcgatccgcg gccgttcctg gccatggcct ccgaatccga gctggcgctg
     5521 cttgcgcgtg cccgctggag cggtcgcagc atcatgcgcg ccgtgcaatg tctggcccac
     5581 gccggcgacg ccgagctgcg ccgctggttc cggcagttcg gtgactggct cgacgccgag
     5641 cacgacatca cgatcgacgg cagcatcgat ccgcagctcg cgctgcagtg gctcggcacg
     5701 caccggccgc aggtggtgcc cggcgtgctg gccgcaagcc ggtcgtggct gcactgaaga
     5761 gcccgcacaa gcgcttcctc cccctgcccg gactccgcca cccggctgct gcgacgcacg
     5821 tttccccgcg cgccgtcccg gtggcatgct gcacgcctcg tcagccacgc gcgcaggagg
     5881 ctgcagcatg aacgacctca ccggctatgc cgtcggcttc atcggcctcg gcctgatggg
     5941 gcgaccgatg gcgctgaacc tgcattgcgc aggcgcgacc gtcaccgtcc acaaccgctc
     6001 gcgcgcgccg gtcgaggcgc tggccgccaa gggcctgcgc accgccgaca gcgccggcac
     6061 ggcggctgcg gccggcgatt tcgtgatcct gatgctgacc gacacgccgg cggtcgacgc
     6121 ggtgctcgaa ggtcccgacg gcgtgctcgc cggcctgcgc cccggcgcgc tggtcatcga
     6181 catgggttcg acctcgccgc tcgcgacgcg cgagttcgcg gcgcgcgtcg ccgaggcggg
     6241 cggcgagtgg ctggatgcgc cggtctcggg cggcgtggtc ggggccgagg cggcgacgct
     6301 cagcatcatg gccggcggca gcatcgaggc gctcgaacgg gcccggccgc tgctcgaacg
     6361 gctcggtgcc cggctcacgc acgtcggccc gagcggctgc ggccagatcg ccaagaccgt
     6421 caaccagatg atcgtcggcc tgaccatcgg cgccgtcgcc gaggcgctgg cgcttgccgg
     6481 gcgcgccggc gccgatccgg cgcgcgtgcg cgcagccctc gtcggtggct ttgcggattc
     6541 acgcatcctc gaactgcacg gcctgcgcat gatcgacggt gacttcacgg cgggcgggcg
     6601 cgtcagcacc cagcacaagg atctgctgca ggcgctggaa ctcgccgggg ccgtcgggct
     6661 ggagctgccg gcgacggaac tgaaccgccg gctgtacgcc gagtgcatcg ccgccggcga
     6721 cggcgggctc gatcacagcg cgctgtatcg ccactacacg cgcaccgcca ccaccggtgg
     6781 cgagggctga agccgatgtc ccgcgacgac gaaaccccga ccgggcggcc gctgcgcctg
     6841 cacgaactgg cggcgagcgc gctgtgggcg ctgctcggcg tgcagaagga agccaaccgc
     6901 cggcgcgact tcgcgcgcgg ccggcccggg cagttcgtgc tgatcggcgt gatcgcgacc
     6961 gtgctgttcg tgctgctgct gatcgggctg gtgcggctcg cgctcggtct ggccggcgcc
     7021 tgaggctaca gcagccggcg caggccctcg agcgccagcc ccggcagacc ttcgaaccag
     7081 ccgtgcgccg gcagcagccg ggcgccgagc tggcggtaca gcccggcatc gagcaggcgc
     7141 tgaccgagtc cgccggccgg cgggcagagc cagtacaggc cgccggccag cgcgtacagc
     7201 gccagcgtgc cggggaggtt gcgcggatcg ccgaagctca gcgccgtgcc gaacgagcgt
     7261 ttcaggcgcc ggccgagcag atcgacgctg tagatctcgt cgagcagcag atgcacgacg
     7321 aagcccgcgc cgaggaagcc cccggccagc caggccgctt cgaccggcag gccgtacagg
     7381 cgctgcgcgg ccagcacggt cgcgagcgcg aaggccgctg cggccggcag cgaatgcacg
     7441 aggccgcgat gcacggtcca gcggttgatc gtcccgaaca cgccatggcg cagcacgacc
     7501 caggcagcgg caccggccag cgtcagctcg gccagtgacc agcgcggcgc cagcgccagc
     7561 accagcgcga aggcgccggc cagcgcgacc aggctgaagg ccacgcgcgc cgggatcgaa
     7621 tgatcgagat cgacatccgg cagcagcgcg ccgatcgcac cgagcgcgaa gcaggccggg
     7681 gcgtgggccg ccgacagcag gccgatgctg agcagcgcgc cggcgccgat gccgcttgca
     7741 gcggtgccga cgatcaggtg ggtgcggaag tcggccaatt tcagtgattc ctcgcaggcc
     7801 ggcaaggcag cgggcgcgga cctcgcggtc cgcgcccggc ggttttcggc agtgggtcgg
     7861 gatgaaaccg tcgcgtcagc cctgctgcag caggttgcgc aggtcgatca gcgcggcgtt
     7921 ggcgcgggcg atgtacgagg ccatgaccag cgagtgattg gcgaacagcg caaagcccga
     7981 gccgttcagg atcaccggcg accacaccgg ctcctgcgtg gcttcgagtt cgtggatgat
     8041 ctggcgcagc gagacctggg cgttcttgtt ggcgagcacg tccttgaagt cgatctcgat
     8101 cgcgcgcagg aattcgatca tcgcccagca gtagccgcgc gcctcgtaga agtggtcatc
     8161 gatctccagc cagctggtct tgatcagctg ctcctgcggc gtcggcgtcg actggcgggc
     8221 gttcgggtcg ccggcgaggt tggtgttgac gcggatgtgc ccgacgctgg ccgacagccg
     8281 ctgcgacagc gagccgaggc gcttctcgac caccgagaag tagtcgcgca gattgtcggc
     8341 gcgcgtgaag aactggccgt cgtcgcgacg ttcgtcggcg agccggtcga ggtagcggcg
     8401 cagcgcccgg atgccgtcgc ggtactcgga ttccgacggc ggcagcagcc aggagttgtt
     8461 gtcgaagttg aacttcggct cggccacctg caggtcgaca tcctcgcgcg actgcgtctg
     8521 cgcgcggctg aagtcgttgc gcagtgaacg cgagatgtcg cggatcgcgg tcagcacgcc
     8581 gaactcccag ttcggcacgt tatccatgaa cacgcccggc ggcatgcggt cgttgctgag
     8641 ataaccaccg ggcttctcaa gcagcgtgtc ggcgacctgg atcacggttg ccgtggtcac
     8701 gtagccggcg acgagcttct tctcgtcgcc gccggccatc gccagcgcga tctcgcgcgg
     8761 ttcgaacagc gacggcttgc tgtcccagat cagccacagc acgccgacga tgatgacgta
     8821 ggtcgccagc agcacgccgg cggcgtacag gatgccccgc tcgcgccagg tgcgcgggtg
     8881 gtaggcgtgg atcagcaccg acagcagcga gcgtggcttg ccgtcgtagg aatcggtctt
     8941 gctcatggat gagttccctg ccttgtgcgt tcttgtcgca gccgttgcga ccgcgtcggc
     9001 agctcgggag tttctggtgc cgggcggtcc ggccgcttcg ggatgggccg gcgcgcgggc
     9061 cggcgtcgac gaaaaagata gcccggccgg gcgggagccg cggccgggcg tgcgggagcg
     9121 tcgtgcaggc ggcgtcgccg ggctcagctg cccttcagct cgcgccggcg cttgtgcagg
     9181 tgcggctcgg tgtagccgtt cggtgtctcg cggccgagga acaccagatc gagcgcggcc
     9241 tggaaggcga tgccgtcgta ggccggcgcc atcggccggt acagcgggtc gccggcgttc
     9301 tggcggtcga cgaccagcgc catgcgccgg aaggtctcca tcacctgctc gcggccgacg
     9361 acgccgtggt gcagccagct ggcgatgtgc tggctggaga tgcgcagcgt cgcgcggtcc
     9421 tccatcaggc cgacatcatt gatgtccggc accttcgagc agccgacgcc ctggtcgatc
     9481 cagcgcacga cgtagccgag gatgccctgg gcgttgttgt cgagttcctg ctgcagctcg
     9541 gcggccgacc agttcgcctc ggccgtgacc ggcggcgtca ggatcgcatc gaggctcgca
     9601 cgcgggcggc tcgccagttc ggcctgacgc gcggcgacat cgacctggtg gtagtgcatc
     9661 gcgtgcagcg tcgcggcggt cggcgacggc acccaggcgg tgttggcacc ggcgagcgga
     9721 tgcatctgct tggtcgcgac catcgccttc atctcgtcgg gcatcgccca catgcccttg
     9781 ccgatctgcg cgcggccgcg caggccgcag gcgaggccga tgtcgacgtt ccagtcctcg
     9841 taggccttga tccaggcggc gttcttgatg tcgcccttgc ggatcaccgg acccatctcc
     9901 atcgaggtgt ggatctcgtc gccggtgcgg tcgaggaagc cggtgttgat gaagatcacg
     9961 cgctcgcggg cggcgcggat gcattccttg aggttgacgg tggtgcgccg ttcctcgtcc
    10021 atgatgccga tcttcagcga gttgcgggcc aggccgagcg catcctcgac gcgcccgaac
    10081 agctcgacgg cgaacgcgac ctcctcgggg ccgtgcatct tcggcttgac gatgtacatg
    10141 ctgccgcggc gcgagttctt caggcggttg aggcccttca ggtcgcggta cgagatggtc
    10201 gcggtgacga gcgcatcgag gatgccctcg ggaatctcgg ccgccccggc gccgaagagc
    10261 accgccgggt tggtcatcag gtggccgacg ttgcgcacca gcagcagcgc gcggcccggc
    10321 agcgtcagcg tcgagccgtc gggggcggtg taggcgcggt cctcgttcag cgcacgctcg
    10381 acgcttttgc cgcccttgtc gaactcggcg gtcagcgaac cggtgatcag cccgaaccag
    10441 ttcgagtaga ccagcgcctt gtcctcgccg tcgacggcgg cgaccgagtc ctcgcagtcc
    10501 tggatggtcg tcagcgccga ttcgagcacg acatccttga cgccggcgcg gctctgcgca
    10561 ccgaccgggt gcttgcgatc gagctggacc tcgacgtgca ggccgtggtg gcgcagcagg
    10621 aaggtcttca gcgcgccgtc ggtctcgacg tagccgacga aggccgaggg atcggccagg
    10681 gcggtcttcg tgccatcggc gagctgcgcg accagcgtcg cgccgtcgcg caggtattcg
    10741 gcgacctcgg cgtgcttgcc gcttgcgagc ggcacggcct ggtcgaggaa gccggcggcg
    10801 taggcgatga ccttgtcgcc gcgcgccggg ttgtagccct tgcccttctc ggcgccgccg
    10861 tcctcgggca gcacgtcggt gccgtacagc gcgtcgtaca gcgagcccca gcgggcgttg
    10921 gcggcattga gcgcgtagcg ggcgttgttg accggcacca ccagctgcgg gccggcaacc
    10981 gaggcgatct ccgggtcgac attgtcggtc gagacctgga agtcggggcc ttcgggcagc
    11041 aggtagccga tctcgcgcag gaaggcgaca taggcggcgt gatcgtgcgg ctggccgcga
    11101 cgcgcgacgt gccaggcatc gatctgcgcc tgcagcgcat cgcgcttcgc cagcagttcg
    11161 cggttgcgcg gggccagatc cgtgacgatg gcttccagcg ccgtccacca gcctgcggca
    11221 tcgaggccgg taccgggcag cagcgtgttc tggaccagat cgtacagggg cttggcaacc
    11281 tgcaggccgc cgatggcaac gcgctcagtc atgtaggttc gtctccggat tgagggcagg
    11341 gctgcggtcg tgcatacgga gggcacgacg gcgaggccgg gcattttcgc cggccgtaac
    11401 ggcttgtgca atgcgcaatg tccgctgccg tccggccccg gatgcagcgg tgcagtggtc
    11461 aggcagattt aaaaagcctg tcacaggcat ccccggtaat gcggggtggc tctggtacga
    11521 tgcgcgcctt catgtgggga gcaagtgtcg tcggggccaa cgacggcttt ctgcaggggt
    11581 gatgtgcgag caggccaggg agcggcaagg cgggcccgcg cgcattgcct cgcctcgcct
    11641 tattccggat ggtccggatt gcatgtggga ctcaggactc gtgaagggca ttcttcaaga
    11701 acattcggtg gttgtcgaag cggtgctgcg cacgctcgac tggcttgcaa ttgccatggc
    11761 cggctggctg gccttcgtgc tgcgcttcga tgctgccgtg atgccgggtc ctttccagcg
    11821 ggcgatgatc atcgcgctgc tggtcagcat cggtgtcttc ggtcgctgca gcgtctaccg
    11881 ggcctggcgt ggcgaaaccc tgaacggcga gctgcgcacg ctgctgtggg cctggtgcct
    11941 gaccttcctg atcacgatcg gtgcgctggc cctcaccacg ggccgcgacg aagcgccgtt
    12001 tccgcgcctc tggctgatgc tgtggttcgt gctcggctgc tgcctgctga cactgctgcg
    12061 cggctgcctg cgccaggtgc tgcgccggct gcgcagcagc ggtcacaaca cgcgccgggt
    12121 ggtcgtggtc gtggccggtg aactcggcgt cagcgtcatc cgccggctgt gcgaggcgcg
    12181 ctggaccgga ctgcgcgtgg tcggctattt cgatgaccgg ccgatcgagc gcgtcggcga
    12241 gatcgccggc gtgccgagaa tcggacgcat cgacgaactg gcggcctacg tcgagggggg
    12301 gcacgccgat caggtctgga tcacgctgcc gttccgggcc gaggagcgcg tgcgcgagat
    12361 cctcgagacg ctgcgccacg cgaccgccga catccgcatg gtgcccgaca tcctcggcct
    12421 gcagctgctg aaccattcga tgggcaccgt cgccggcatc ccggtcatca acctgtcctc
    12481 gacgccgatg gtcggcttca atcgcgtcat caaggcgctg gaggatcgca tgatctcgct
    12541 ggcgatcctg ctgatgatcg cgccgctgat gatcgcgatc gcgatcggca tcaagctgtc
    12601 atcgccgggg ccggtgttct tccgtcagga gcggctcggc tacgacggcc ggccgttccg
    12661 ggtctggaag ttccgcagca tgaaggtgca tcaggagcac ggcggccagg tcacgcaggc
    12721 gacgcgcggc gattcgcgga tcacgccgtt cggtgccttc ctgcgccgca ccagcctcga
    12781 cgagctgccg cagttcttca acgtgctcga cggcagcatg tcgatcgtcg gcccgcgccc
    12841 gcacgcggtc gcgcacaatc aggaatacaa ggaactcgtc gacagctaca tgcgccggca
    12901 caaggtcaag cccggcatca ccgggctcgc gcaggtccgt ggctaccgcg gcgagaccga
    12961 tacggtcgac aagatgaagc tgcgcgtcga gagcgatctc gaatacatcg agaactggtc
    13021 actcggcctg gacctgaaga tcatcctgca gaccatcttc aaggggttcg tgaatccgaa
    13081 tgcctattga accgtccacc acctgcgctg cggcatggat cggcgaggcg atgggagtac
    13141 ggggcatcgc atgaagcgcg ccctcgtcgc cgaatcttcg gtcatgcgcg gcctgctggc
    13201 gcgcagcctc gatatcgcca cgatcgtggc gagcagcttt gccggctggt ggctggagac
    13261 cggcagcacc ggctggttcg aaggggattt cctgcgcgcc gccctgatcg ggctgctgct
    13321 gaccggcatc tgctctgcgc actgggcggt gtaccgcagc tggcgcggca cgccgctgcg
    13381 cagcgagctg gaggccgtgc tcggtgcctg gctgctggcc gtgctgctgc tggtgccggt
    13441 gctcgaactg accggcgagc cggacgaata cggccggcac tggctgctgt actgggcgct
    13501 gttcggcacg gccgggctgg ccggctggcg ggtgctgatg cgtcgcgcgc agcgctacgt
    13561 ccataccggt ggtcgcgacg tgcgccgcgt ggtgctggtc accaacggcg gcttcggatt
    13621 gcagatcatc gagcagctcg ccaccgcacg ctgggccggc ttcgaattcg tcggctggtt
    13681 cgatgaacgc gacggcgatg acggccggat cagcgcagcg gccgtgcagc gctgcctggg
    13741 cccgaccgag cggctggcgg gcttcgtcga ggacggcaat gccgacgagg tctggatcgc
    13801 gctgcccttc cgggccgagg ccaggctgcg ctcgatcctc tggagcctgc gccactgcac
    13861 ggccgacatc cggctggtgc cggacatctc cagcttccgg ctgctgaacc attcgctcgg
    13921 cgaggttgca ggtctgccgg tcatcaacct gtcctcgacg ccgatgagcg gccgcaaccg
    13981 cctgctgaag gccatcgagg accgcacgct cgccggcgcc tgcctgatcc tcgctgcacc
    14041 gctgctgctc gtgatcgcac tcggggtcta cctgaccacg ccggggccgc tgttcttccg
    14101 gccggtacgc ctcggctatg acggccggcc gttccgcgcc tggaagttcc gctgcgcggt
    14161 gccgcacgcc ggccgcaacc gcttcacgcg cttcggcgtg ttcctgcgcc acaccggtct
    14221 cgaaggcctg ccgcagctgc tgaacgtgct cgacggcagc atggccatcg tcggaccgtg
    14281 tccgcacgcc gccgcctaca acgcccagta caaggcacgg gtcgacggct acatgagccg
    14341 tttcaaggtc aagccggggc tgaccggctg ggcgcagatc catggccacg accagcgcat
    14401 cgaatcgatc gaggacatgg agcggcgtct ggcgctcgat ctcgaataca tcgagaactg
    14461 gtcgctggaa ctcgacctgc gcatcgtgct gctgacggtc tggcagatgg ccgggcgcgt
    14521 gctcgggggg ctgcgcaagg ccgccgccca gcagatccgc cactaacgcc gctgccagcg
    14581 gccggctgcg tccatcacgt actgtccgtc agccgcgccg gccgtcagcc ggcggccggc
    14641 gagcgcctgc acttcggcga gcgggcggcc gttgcgctcg gcgatgtcgc ggtaacgggc
    14701 catgcgctcg gcgttgacac gctcgaccag cgcgcgcgcg ccggcatccg gcgtgaccgc
    14761 gccgatcagc ccgtcgggcc gctcgccgac ccggccggcc gcgagggcat cggccagcgg
    14821 ttcggccagg accggcgggc tgccggcggc cagcagcacg cagagggcgg tgacgggcag
    14881 cagccggcgg cggtggacgg ggctcatgga cgggactcct gcggtttgcg cgccttcgcg
    14941 gccggcccga acagctccgg gtgatcgccg aaggcggtgt cgagatcgcg atcgagcttc
    15001 acgcgcacat cctgctggat gtgcacattc aggttgatct cgatcggctt gtccggtgcc
    15061 tcgaccttca cggtcggcgt gcaggccgca caggccgcac cgatcgccag cagcgccacg
    15121 atcgcgacga tcggcggccg gtgccggcac gggctcgaag gaaacacggg gttgcgcagg
    15181 atcatgggtt ctcccggtcg gatgattcga ggcgccggcg cacggtttcg ggcagccggt
    15241 agctgtccag gccctgctgc agcagacctg ccagcgcgcc gctgaggttc aggttcaggg
    15301 ccaccggatg gccgccgtag gcctgcggat tggctccccg cacgttcagg ccggcggcca
    15361 gcgattccgc ccgctccccg gccagcgtca ggctcaggga ttcgtagcgg aaatcgcgca
    15421 gcacatcgag cacgaagccg gcctggccat cggcgagcgc ggccggcggc gtcgatggcg
    15481 tgtagtgcag cgtaccggag ccatcgctcg cgaactgtgc ctgcacgtca tggaggccat
    15541 cggcttcgag ccgggccgcg aggcttccgt tcagcgtgcc ggtgccgccc aggccatcga
    15601 ccggcacgat tgcgagcagc cggctcagat cgagcccgcg ggcgctggcg gcgagcgggc
    15661 ggggcgccgg gccgtcgagg cgcagcggtg ccagctccag ccggccgccg gccagcgtga
    15721 agccggcctg acgcaggcgc agctgcgtgt cgtcgagcgt gaattcgacc cgcccgtcct
    15781 cgaacggcag accggcgtcg agccgggcga tcctcagggg ctggccggcc gggatgcgca
    15841 gcggccacag ccggtcggcg cgcagcacgc cgtcgatgcc gcgggcgcgc acgccgccga
    15901 agcgcagctc caggccgtcg atgcgcagct cgcccccgct cctcgcggcc gccccgccgc
    15961 cccagtccgc ccgcgcctgc agccgcaggc cgccgccggg cacgctgagc tgcgccgcga
    16021 gcgccggcga cagcgcggcc agcgccacgg ccgggggtgc cgtgccgaag cgcaggtgca
    16081 ggagatcgag cttcagttcg ccg
//
LOCUS       DAOQIE010000044        16000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1721_length_16000_cov_8.076638, whole genome shotgun sequence.
ACCESSION   DAOQIE010000044 DAOQIE010000000
VERSION     DAOQIE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 16000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16000
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_16000_cov_8.076638"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK144_04475"
     CDS             <1..469
                     /locus_tag="PK144_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNF65016.1"
                     /translation="TRMLAERLDALSPLSVREGSDGARIQPGEIWIAPGDFHMTVRRS
                     ADGLHLGLNQGAPENSCRPAVDVLLRSAVAACGARVLGVILTGMGHDGLASSTRLREA
                     GGQLLAQDERSSVVWGMPGNVVRAGLADRVLAIEDIGPEIAARVRGEGGRHGC"
     gene            463..1281
                     /locus_tag="PK144_04480"
     CDS             463..1281
                     /locus_tag="PK144_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNF65017.1"
                     /translation="MLTATDFDYVRRLVLERSAILLEPGKEYLVELRLKPIVRMRGLR
                     DLGELVQLLRTDVSGSLQREVVEAMTTNETSFFRDVHPFETLRQSVLPELLRNRAAER
                     VLNIWCGAASSGQEVYSIAMTLREHFPELAHWRVSIVATDLSREMILRCREARYSQLE
                     INRGLPANLLIKYFERADMDWRIREDVRRMIDFREMNLAGNWAGLPVCDLVFLRNVLI
                     YFDDATKRQILGKVRGLMRPGGYLFLGSSETTLNLDSRFEQVKFDRTLCYRVRA"
     gene            1292..1735
                     /locus_tag="PK144_04485"
     CDS             1292..1735
                     /locus_tag="PK144_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNF65018.1"
                     /translation="MNIGPNEIGEIVASVWATMLGGELFALPGSIELEGELISGEIHM
                     RGEDGFEGLVRIVASLPVAQAAARVMFTTDTPDDRDATDTIAELINMIGGNIKSLIAE
                     DARLDLPKVTHGQPDTSADAAAIARADADFIAADHPFRVTLLRMR"
     gene            1846..2205
                     /locus_tag="PK144_04490"
     CDS             1846..2205
                     /locus_tag="PK144_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65019.1"
                     /translation="MKILVADDSKAMRSIVIRTLRQAGYSGHKVIEAENGAQALELAK
                     DEEPDLIVSDWNMPEMNGLEFLKALRAAGMDTPFGFVTTEASPEMRALADENGAAFLI
                     TKPFTAEDFQKHFDEYL"
     gene            2234..2701
                     /locus_tag="PK144_04495"
     CDS             2234..2701
                     /locus_tag="PK144_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65020.1"
                     /translation="MLRLPTPDTVTRLLSGLLGVKVGVVDNKVDDAKLVVCGVFASAV
                     HHEAAFCLLDIPFGAAAGAALSMMPADVAKEYVKKGKLDEDIRDNCAEVLNVCSRLFT
                     SSDAHRVTLTVKTFHPEPIPAEVKAALAGSQRLNLNLSVDRYGGGALVLIIPG"
     gene            complement(2705..4663)
                     /locus_tag="PK144_04500"
     CDS             complement(2705..4663)
                     /locus_tag="PK144_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNF65021.1"
                     /translation="MFPTRLRGLPVALLLALTAPIAADPAAAAPLSETALAAQRQSYL
                     DAERALAAGDRTRMRTLSPQLRDYPLYPYLRARELGQNLATASSAEIRQFLSQWADVP
                     AAARLRGAWLRALAEQSRWDDVIADYRPGPDPELDCLYRTALLNRDRPDVALNNFQAL
                     WLREQPLPAACDRPVQAWASQGGLTPALAWQRFAALMGARNPAAARPVQTWLEPADQA
                     WARLWLQVDQNPALILQAPDLQMAGSDRGAGIVTWGLARWIERNSAEAAPAFDEIRRR
                     GLIADEQLLPLERRLAVLLAARRHPDALRRLDALGSSREDDSTREWRARLGLAQGDWP
                     LVLRSIEHMPAAQAAQAGWRYWRARALESSGQNGTAERLYRELAGERDYYGLLAARRI
                     GTAPAWTQRPLPRPPVATLRGLAARPAVQRARELFLLGRPGEARSEWEPVLRNADTAT
                     WRAAALLAADWNWYGQTIIWLARAGDWDDLVLRFPTPWRSEFENAAQDTQLPPAWLYA
                     VARVESTLLPEARSGAGALGLMQLLPDTARGVASSIGYRLGDNSQLFDPAVNIRLGSV
                     YLRQLLDRFGGNPVLATAAYNAGLRRVGEWLPAGTELAADVWIDTIPYSETRAYVRRV
                     WEYMAIYESRLGGTAHGFEVERIEARAP"
     gene            complement(4674..5333)
                     /locus_tag="PK144_04505"
     CDS             complement(4674..5333)
                     /locus_tag="PK144_04505"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNF65022.1"
                     /translation="MQHRILFLCLGSLCALPAAADICSYRDAQGVLHISNVCDGDSRY
                     RVTMATPSYANRPDAPSDYRPATAQLPPGVSLGHLPTTRVASSARRKLYSPAIHAVAA
                     QYRLEPALLHAVITAESAYDPLAVSRAGAMGLMQLMPATARRFGVSDAFDPTENLHGG
                     ARYLRLLLDQFQQMTLAIAAYNAGENTVIRNGYAVPPIEETRVYVARVLDLYDRYRRE
                     Y"
     gene            5546..7096
                     /gene="pap"
                     /locus_tag="PK144_04510"
     CDS             5546..7096
                     /gene="pap"
                     /locus_tag="PK144_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNF65023.1"
                     /translation="MFRTAELDHSLDKTEYLRREAELRQELLTLQGELREADFPVLLV
                     FAGVDGAGKGETVNQLNEWLDTRLLLTRAFDQPSDEERERPLYWRYWRELPARGRIGM
                     YLSSWYSAPLLDRVYERIGVETFDERLDRIRNFEEALAHDGALILKFWMHLSAPAQEK
                     RLKKLERDPLLKWRVTARDWEHWRLYPRFIAAAERALMRTGTGDAPWKIVEGFDERYR
                     IVTVATILRDAIRRRLAERRLLHEVVQQVNDGGGETLQALVEGSAVLPGQPTVLDSLD
                     LGKALPKAEYRERLLKLQGRLNRLQRRARERGVSTVLVFEGWDAAGKGGAIRRITSAI
                     DARDYQVVQVAKPTDEEAARHYLWRFWRYMSRAGRMTIFDRSWYGRVLVERVEGFARP
                     AEWRRAYAEINDFEDHLLEHGIVLVKFWLHLSRGEQQRRFETRAATPWKSWKLTDEDW
                     RNRERWQDYEHAVNDMVEHTSTRQAPWVLVEAEDKYHARVCVLEHVCEHLERGLKRAA
                     RRNGTSAG"
     gene            complement(7187..7864)
                     /locus_tag="PK144_04515"
     CDS             complement(7187..7864)
                     /locus_tag="PK144_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNF65024.1"
                     /translation="MNSYQPLPVGARTGAPSTVQGHRVLRNTYLLLSMTLLFSAATAG
                     FAVVTQAPPMHWLLMLVGMFGLLFLTMKLRNSAWGLASVFAFTGFMGYTLGPVLSRYL
                     ALPGGGELVMGAMGLTGLIFLSLSAYVLVSRRDFSFMGGFLFVGLIVALVASLAAMFF
                     SVPGLHVAVSAMVVMLMSGFILYDTSSILHGGETNYIMATVALYLDIFNLFTSLLQLL
                     GFLGSDD"
     gene            8116..8367
                     /locus_tag="PK144_04520"
     CDS             8116..8367
                     /locus_tag="PK144_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNF65025.1"
                     /translation="MQVLLAGLLLIASNGAWAQVFKCVDAAGLTSYSQTPCTADAREV
                     GRWQGRVGAVMIPAGAAAVTMRPVDVNRTKLPVQAPSQP"
     gene            complement(8378..9502)
                     /gene="zapE"
                     /locus_tag="PK144_04525"
     CDS             complement(8378..9502)
                     /gene="zapE"
                     /locus_tag="PK144_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNF65026.1"
                     /translation="MPYSRYQQDLKRPGFAHDPAQEHAVRLLQKLHDALIARPQPAAV
                     PVRSGGGLFGKLLGKSRETDAPTAPAIRGLYFWGGVGRGKTYLVDTFYDTLPFREKQR
                     LHFHRFMQMVHARLKDVKHQQDPLKIVAAQLAENLRVLCLDEFFVADITDAMLLHGLL
                     AALFERGVVLVTTTNIPPDLLYRDGLQRARFLPAIELLKTHCEVVNVDGGTDYRLRYL
                     ERAEIYHHPLDARAETVLAETFRSLAPEPGQHNARLEIEGRFLRTRELADGVVWFDFA
                     EICDGPRSQNDYIEIARQFHTVLIGRVPVLDWTMENAARRFLNLVDEFYDRNVKLILA
                     AAAPCEAIYVGEKLKFEYQRTISRLQEMQSKEFLARPHLP"
     gene            complement(9571..10389)
                     /gene="cysQ"
                     /locus_tag="PK144_04530"
     CDS             complement(9571..10389)
                     /gene="cysQ"
                     /locus_tag="PK144_04530"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNF65027.1"
                     /translation="MDTALAALAEPVLALAMRAAGEILRIYASDFAIHLKSDRTPVTA
                     ADLAAHAVLCEGLARLTPDIPVLSEESAPDEHPQRLGWPRLWLVDPLDGTREFVARSG
                     EFSINIALVEDHLPVFGLILMPVSGAAYWAWRGGGAWKLVPGSTPVPIRCRALGSGPV
                     RVAGSRRHTGHLLQTYLGYLGEHVYRGIGSALKACLIAEGEADVYPRFGPTSEWDTAA
                     SQILLEEAGGGLCDGARRPLRYNARHTLINPDFLAFGDAGHDWTRYLPRRSPPA"
     gene            complement(10410..10958)
                     /gene="nudE"
                     /locus_tag="PK144_04535"
     CDS             complement(10410..10958)
                     /gene="nudE"
                     /locus_tag="PK144_04535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HNF65028.1"
                     /translation="MRPLPEILATETVARSRLFRVEKVDLRFANGVEVEFERVPSGGH
                     GSVLIVPMLDADTCLLVREYAAGTHRYELGCPKGVVEAGEDPLAAANREIMEEVGHGA
                     RRLTLLRSATIAPGYFAHVTHLVLAEDLYPETRPGDEPEAIEVVPWRLSELDALLARP
                     DFTEARSIAALFLARAALAAAR"
     gene            11053..11730
                     /gene="yrfG"
                     /locus_tag="PK144_04540"
     CDS             11053..11730
                     /gene="yrfG"
                     /locus_tag="PK144_04540"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HNF65029.1"
                     /translation="MASPPWTRIDTVLLDMDGTLLDLRFDNHFWLEHLPARWAALHGR
                     EPAAAREELLARYREVEGTLAWYCVEHWSRALAVDVVELKREVEHLIRFLPDSLDFLA
                     ALRQAGKRVVLATNAHPYSLALKAEKTGLGGHFDVLVSAHEFGVPKEHPKFWASLQAR
                     IGYDPPRTLFLDDSLPILRAARDYGHIGHLVSMLRPDSAQPLRARAEFAAVLRLGELL
                     PVLPSGE"
     gene            complement(11823..13070)
                     /locus_tag="PK144_04545"
     CDS             complement(11823..13070)
                     /locus_tag="PK144_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNF65030.1"
                     /translation="MHHTRPVRRLLAAAALCVLAACASVDPKPSGPYVRQGPFPAPEN
                     LRAPVEFWKQVYGSWSLGQYALHDERYLDLVYEIVDLPGGAREGLSTDDKSVLRARRD
                     GLQAQLRELERKQLAGESLNADEKRLRERIVASSAGEAGVIGAAERLRAQRGIRERFA
                     RGLEIGARYDQRFREIFQSAGVPEDLAYLPHVESSFQASARSSVGAAGIFQFMPSTAK
                     SYMVVHTATDERLDPLVAAQGAARYLADAYSRLGDWGLAITSYNHGVGGLAKARSLHG
                     ADIGRIVWDYDGKAFGFASRNFYAQFLAARELAGRTVVTPARGIPVPRENVVLDHPTS
                     APHLASYYGLSVEQLSALNPGWSPAALGGRAMLPAGATVWVPDQTLARLAAAGQPATP
                     AELIAMADPPRTAAAPARKRGVR"
     gene            complement(13214..13666)
                     /gene="gspG"
                     /locus_tag="PK144_04550"
     CDS             complement(13214..13666)
                     /gene="gspG"
                     /locus_tag="PK144_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNF65031.1"
                     /translation="MPTRLRRRSSSFGFTLIESLAVLIILLVAAALIVPRVMERPDEK
                     RQRAARAEIRTLQEALNRYKKDTGRYPATAVGLEALAHKPAEAAESMNWHGPYLPQVP
                     KDPWNRFYLYANPGQHGPIDIYSLGGDGHPGGEGIDADIGNWNLDEPR"
     gene            13885..14676
                     /locus_tag="PK144_04555"
     CDS             13885..14676
                     /locus_tag="PK144_04555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNF65032.1"
                     /translation="MLAHPVPSPSIATEAVVAPLAEARRAPRLSVQLAVSKAEVREAQ
                     RLRHAIFVEEMGARLHNTLPGHDDDAFDPHCRHLLVRETAGGQLIGCTRLLTDTAARA
                     AGGFYSETEFELGPVRGLAGRIAEIGRTCVHREHRNGATIATLWAGIAGFVAEHEVRY
                     LIGCASVPLAEDMAQRVFSELAAQHLTEDTLRVRPRQPLPRRDLDARGRAVLPPLLKA
                     YLRVGARIAGEPCVDADFGCADLFILLDTVQLERRYARHFLDRPQ"
     gene            14673..15452
                     /locus_tag="PK144_04560"
     CDS             14673..15452
                     /locus_tag="PK144_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF65033.1"
                     /translation="MSAAGLMRVLRRAWRLPLLILLLGLGCAIAACLPGGAGRVPSPA
                     PMVWWSRRLCRLLGVRIVCHGTPLAGRALFVANHVSWLDIMVLAAVCPTHFLAKSEVA
                     GWPLFGWLCRRVGTAFIRRGAGDGAQAAAAELVWRLQRHDGRVLVFPEGTSTDGRGVR
                     RFHARLLQAAERAQCPVQALALRYPVAGGGTHPAVPFIGDDELLAHLWRLLGEQRIEA
                     EVSCLAPLPAGIGRDALARRSHAQIAAVLAGDDPQPRRAIG"
     gene            complement(15469..>16000)
                     /locus_tag="PK144_04565"
     CDS             complement(15469..>16000)
                     /locus_tag="PK144_04565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005307291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF65034.1"
                     /translation="LKGGACPAVADLTAGLGRDACVLAALGCRVTLVERSPVVCALLD
                     DGIARAAADTGIGSWVRERVQLVHADGSAWLQAAAQQAQAAPAQRPEVIYLDPMYPHR
                     EQSALVKKEMRAFRAVVGDDLDADALLAPALAVALRRVVVKRPKGAPPLAGRAPSHVI
                     ASPNTRYDVYVTLAGA"
BASE COUNT         2430 a         5379 c         5842 g         2349 t
ORIGIN      
        1 cacgcgcatg ctggccgagc ggctcgatgc gctgtcgccg ctgagcgtgc gcgagggcag
       61 cgatggcgcg cgcatccagc ccggggagat ctggatcgcg cccggtgatt tccacatgac
      121 ggtgcgccgc tcggccgatg gcctgcatct ggggctcaat cagggggcgc ccgagaactc
      181 ctgccggccg gcggtcgacg tgctgctgcg ctcggcggtg gccgcctgcg gcgcccgcgt
      241 gctcggcgtg atcctgaccg gcatgggcca cgacggcctg gcctcgtcga cgcgcctgcg
      301 cgaggccggc ggccagctgc tcgcgcagga cgagcgcagc agcgtcgtct ggggcatgcc
      361 gggcaacgtc gtgcgggctg gactcgccga tcgcgtgctg gcgatcgagg acatcggccc
      421 ggaaatcgcc gcccgcgtgc gcggcgaagg cggcaggcac ggatgctgac cgcgaccgac
      481 ttcgactacg tgcgccggct ggtgctggag cgctcggcga tcctgctcga accgggcaag
      541 gaatacctgg tcgagctgcg cctgaagccg atcgtgcgca tgcgcggcct gcgtgatctc
      601 ggcgagctgg tgcagctgct gcgcacggac gtgagcggca gcctgcagcg cgaggtcgtc
      661 gaggcgatga cgaccaacga gacctcgttc ttccgcgacg tgcatccgtt cgagaccctg
      721 cgccagtcgg tgctgccgga actgctgcgc aaccgcgccg ccgagcgtgt gctcaacatc
      781 tggtgcggcg ccgcatcgag cgggcaggag gtctactcga tcgcgatgac gctgcgcgaa
      841 cactttccgg aactggcgca ctggcgcgtg tcgatcgtcg ccaccgatct ctcgcgcgag
      901 atgatcctgc gctgtcgcga ggcccgctac agccagctcg agatcaaccg cggcctgccg
      961 gccaacctgc tcatcaagta tttcgagcgg gccgacatgg actggcgcat ccgcgaggac
     1021 gtgcgccgca tgatcgactt ccgcgagatg aacctggccg gcaactgggc cggcctgccg
     1081 gtctgtgatc tcgtcttcct gcgcaacgtc ctgatctatt tcgacgatgc gaccaagcgc
     1141 cagattctcg gcaaggtccg cggactgatg cggccggggg gctacctgtt cctcggcagt
     1201 tccgagacga cgctgaatct ggacagccgg ttcgaacagg tgaagttcga ccgcaccctc
     1261 tgttaccgcg tgcgagcctg aaggagacca aatgaacatc ggaccgaatg aaatcggtga
     1321 aatcgtggcc agcgtctggg caacgatgct cggcggcgaa ctgttcgcgc tgcccggcag
     1381 catcgagctc gaaggcgagc tgatcagcgg cgagatccac atgcgcggcg aggacggctt
     1441 cgaaggtctg gtgcggatcg tcgccagcct gccggtggcg caggcggccg cccgggtcat
     1501 gttcacgacc gacacgccgg acgatcggga cgccaccgat accatcgccg agctgatcaa
     1561 catgatcggc ggcaacatca agtcgctgat cgccgaggac gccaggctgg acctgccaaa
     1621 ggtcacgcac ggccagcccg acaccagcgc cgacgcggcg gcgatcgccc gcgccgatgc
     1681 cgatttcatc gcggccgacc atcctttccg ggtcacgctg ctgagaatgc gataaaattc
     1741 cgcgttccgg cgggggtgtt tcccgccatc ggccggctgc cgcggcacac ccgaggcccg
     1801 gccgcaccga ccctacccgg aacccaacag cggagcattg atttcatgaa gattctcgtt
     1861 gccgacgaca gcaaggccat gcgcagcatc gtcatccgta ccctgcgtca ggccggctac
     1921 agcgggcaca aggtcatcga ggccgagaac ggcgcccagg cgctcgaact ggccaaggac
     1981 gaggagccgg acctgatcgt ttccgactgg aacatgccgg aaatgaacgg tctcgaattc
     2041 ctgaaggcgc tgcgtgcggc cggcatggac acgccgttcg gcttcgtgac caccgaggcg
     2101 agccccgaga tgcgcgcgct cgccgacgag aacggtgccg ccttcctgat caccaagccg
     2161 ttcaccgccg aagacttcca gaagcacttc gacgaatacc tctgagcttt tcctgcgcca
     2221 gcgaggaccc gccatgctcc gactgcccac gcccgatacc gtcacccgcc tgctgtccgg
     2281 cctgctcggg gtgaaggtcg gggtcgtcga caacaaggtc gacgacgcca agctggtcgt
     2341 ctgcggcgtg ttcgcgtcgg ccgtccatca tgaagcggcc ttctgcctgc tcgacattcc
     2401 gttcggggca gcggccgggg cagcgctgtc gatgatgccg gctgacgtgg ccaaggaata
     2461 cgtcaagaag ggcaagctcg acgaggacat ccgcgacaac tgcgccgagg tcctcaatgt
     2521 ctgttcgcgg ctgttcacgt cctccgacgc gcatcgcgtg acgctgacgg tcaagacctt
     2581 ccaccccgag ccgattccgg ccgaggtcaa ggcggcgctg gcgggcagcc agcggctgaa
     2641 cctgaacctc agcgtcgatc gctacggtgg cggcgcgctg gtgctgatca ttcccggctg
     2701 agcctcacgg cgcgcgggct tcgatcctct cgacctcgaa accgtgcgca gtgccgccga
     2761 ggcggctctc gtagatcgcc atgtactccc agacacgccg cacgtaggcg cgggtctcgg
     2821 agtaggggat cgtgtcgatc cagacatcgg ccgcgagttc cgtgccggcc ggcagccatt
     2881 ctccgacccg gcgcaggccg gcgttgtagg cggctgtcgc cagcacggga ttgccgccga
     2941 agcgatccag cagctggcgc aggtagacgc tgccgaggcg gatgttgacg gccggatcga
     3001 acagctggct gttgtcgccc aggcggtagc cgatgctgga cgccacgccg cgggcggtat
     3061 ccggcagcag ctgcatcagt ccgagggcac cggcaccgga gcgcgcttcg ggcagcagcg
     3121 tgctctcgac gcgcgccacc gcgtacagcc aggccggtgg cagctgcgtg tcctgcgcgg
     3181 cgttctcgaa ttcgctgcgc cagggggtcg ggaagcgcag caccagatca tcccagtcgc
     3241 cggcacgcgc aagccagatg atcgtctggc cgtaccagtt ccagtcggcg gcgagcagcg
     3301 cggcggcgcg ccaggtggcg gtgtcggcat tgcgcagcac cggttcccat tcactgcgcg
     3361 cctcgcccgg gcgtccgagc aggaagagtt cccgggcccg ctgcacggcc ggtcgcgcgg
     3421 cgaggccgcg cagcgtggcg accggcggac gcggcagtgg tcgctgcgtc cacgccgggg
     3481 ccgtgccgat gcggcgggcg gcgagcaggc cgtaatagtc gcgctcgccg gcgagttcgc
     3541 ggtacaggcg ctcggccgtg ccgttctggc cgctgctttc gagtgcccgt gcccgccagt
     3601 agcgccagcc tgcctgtgcg gcctgcgcgg ccggcatgtg ttcgatcgag cgcagcacca
     3661 ggggccagtc gccttgggcg agaccgaggc gcgcgcgcca ttcccgggtc gagtcatcct
     3721 cgcggctgct gccgagggca tcgagccggc gcagcgcatc ggggtggcgg cgcgcggcga
     3781 gcagcaccgc cagccggcgt tcgagcggca gcagttgctc atcggcgatg aggccgcgac
     3841 ggcggatctc gtcgaaggcc ggcgcggcct cggcgctgtt gcgctcgatc cagcgcgcca
     3901 gcccccaggt gacgatgccg gcaccgcggt cactgccggc catctgcaga tccggagcct
     3961 gcaggatcag ggccgggttc tggtcaacct gcagccagag ccgtgcccag gcctgatcgg
     4021 ccggttcgag ccaggtctgc accggacggg cggctgccgg gttgcgggcg cccatcaagg
     4081 cggcgaagcg ctgccaggcg agggccggcg tcagcccgcc ctgactggcc caggcctgca
     4141 ccggccggtc acaggcggcc ggcagcggct gttcgcgcag ccacagcgcc tggaagttgt
     4201 tgagcgcgac atcggggcga tcgcgattca gcagggccgt gcggtacagg cagtcgagct
     4261 cgggatccgg gccggggcgg taatcggcga tcacgtcgtc ccagcgcgac tgctcggcca
     4321 gcgcccgcag ccaggcgcca cgcaggcggg cggcggccgg cacgtcggcc cactggctca
     4381 ggaactggcg gatctcggcg ctggaggccg tggcgaggtt ctggccgagt tcccgggcac
     4441 gcagataggg gtagagcgga tagtcgcgca actgcggcga cagggtgcgc atgcgcgtgc
     4501 gatcgccggc ggcgagggca cgctcggcgt cgaggtagct ctggcgctgg gcggcgaggg
     4561 cggtttcgct gagcggcgcg gccgcggccg gatctgcggc gatcggtgcg gtgagggcga
     4621 gcagcagcgc gaccggcagg ccgcgcaggc gggtggggaa cacgacggag gactcagtat
     4681 tcgcggcggt aacggtcgta gagatcgagc acgcgcgcga cgtagacgcg ggtttcctcg
     4741 atcggcggca cggcgtaccc gttgcggatc acggtgttct cgccggcgtt gtacgcagcg
     4801 atcgccaggg tcatctgctg gaactggtcg agcagcaggc gcaggtaacg ggctccgcca
     4861 tgcaggttct cggtcggatc gaaggcatcg ctgacgccga aacgcctggc ggtcgccggc
     4921 atcagctgca tcaggcccat ggcgccggca cgcgacacgg ccagcggatc gtaggcggat
     4981 tcggcggtga tcaccgcatg cagcagcgcc ggttcgagcc ggtactgggc tgcgacggca
     5041 tggatggccg ggctgtagag cttgcggcgg gccgagcttg cgacgcgcgt ggtcggcaga
     5101 tgaccgaggc tgacgccggg cggcagctgg gcggtcgccg gccgatagtc ggacggtgcg
     5161 tcggggcgat tggcgtagct tggcgtcgcc atcgtcacgc ggtagcgcga gtcgccgtca
     5221 cagacgttgc tgatgtgcag cacgccctgg gcgtcgcgat aggagcagat gtcggccgct
     5281 gccggcaggg cacacagact gccgagacac aggaaaagta tccggtgctg catggtgcga
     5341 ggttcatccg gctgcgacgt ttgccgcgga cgggtgtccg gggcgccgtc tattctggtc
     5401 ggggcgcccg gcggggggcc ggggctacgg cagcagcaaa gtactggatg gcggcagcgg
     5461 acacaaccgt ctgtcgccat tgaggttcgg gcgccgttca tttcctctga gggtccgcgt
     5521 ccccggcggg ccatggcgga cagcgatgtt cagaaccgcg gaactcgatc attccctgga
     5581 caagaccgaa tacctgcgcc gtgaggccga gctgcgtcag gagctgctga cgctgcaggg
     5641 cgaactgcgc gaggcggatt ttccggtcct gctggtcttc gccggcgtcg acggtgccgg
     5701 caagggggag accgtcaacc agctcaacga atggctggac acgcgcctgc tgctgacgcg
     5761 cgccttcgac cagccttcgg acgaggagcg cgaacggccg ctgtactggc gctactggcg
     5821 cgagctgccg gcgcgcgggc gcatcggcat gtacctcagt tcgtggtact cggcgccgct
     5881 gctcgatcgg gtctacgagc gcatcggtgt cgagaccttc gacgagcgcc tcgaccgcat
     5941 ccgcaacttc gaggaagccc tcgcgcacga cggggcgctg atcctgaagt tctggatgca
     6001 tctttcggca ccggcgcagg agaagcggct gaagaagctc gaaagggacc cgctgctgaa
     6061 atggcgggtc acggcccgcg actgggagca ctggcgcctg tacccgcgct tcatcgccgc
     6121 tgccgagcgg gcgctgatgc gcaccggcac cggcgatgcg ccgtggaaga tcgtcgaggg
     6181 gttcgacgag cgctaccgca tcgtcacggt cgcgaccatc ctgcgggatg cgatccgccg
     6241 ccggctcgcc gagcggcgcc tgctgcacga agtcgtgcag caggtgaacg atggcggcgg
     6301 cgagacgctg caggcgctgg tcgagggcag cgccgtgctg cccggacagc cgaccgtgct
     6361 cgattccctc gatctcggca aggccttgcc gaaagccgag taccgcgaac gcctgctgaa
     6421 gctgcaggga cgcctcaacc gcctgcagcg ccgggcgcgc gagcgcggcg tatcgacggt
     6481 gctggtgttc gagggctggg atgctgccgg caagggcggt gcgatccggc gcatcaccag
     6541 cgccatcgat gcacgcgact atcaggtcgt gcaggtcgcc aagccgaccg acgaggaggc
     6601 cgcgcggcat tatctgtggc ggttctggcg gtacatgagc cgcgccgggc gcatgaccat
     6661 cttcgaccgc tcctggtacg gccgcgtgct ggtcgagcgc gtcgagggct tcgcccgacc
     6721 ggcggagtgg cggcgcgcct acgccgagat caacgacttc gaggatcacc tgctcgaaca
     6781 cggcatcgtg ctggtgaaat tctggctgca tctgagccgc ggggagcagc agcggcgctt
     6841 cgagacacgg gccgcgacgc cgtggaagtc ctggaagctc accgacgagg actggcgcaa
     6901 tcgtgagcgc tggcaggact acgagcatgc cgtcaacgac atggtcgagc acaccagtac
     6961 ccgccaggcg ccgtgggtgc tggtcgaggc cgaggacaag taccacgcgc gcgtctgtgt
     7021 gctcgaacac gtctgcgagc atctcgaacg cggtctcaag cgcgccgccc ggcgcaacgg
     7081 gacttcggcc ggctgaggag gccggccggg ctccggcgcg ggccggccag aaaaaagccc
     7141 cgctgcagcg gggcttgact ggctacgacc cgtgccgcag cagcgatcag tcgtcgctgc
     7201 cgagaaagcc gagcagctgc agcaggctcg tgaacaggtt gaagatgtcc agatacagcg
     7261 cgacggtcgc catgatgtag ttggtctcgc cgccgtgcag gatcgaactg gtgtcgtaca
     7321 ggatgaagcc cgacatcagc atcacgacca tcgccgaaac cgcaacgtgc aggcccggca
     7381 cggagaagaa catcgccgcg aggctcgcga ccagcgcgac gatcaggccg acgaacagga
     7441 agccgcccat gaagctgaag tctcggcgcg acaccagcac gtaggccgac agcgacagga
     7501 agatcaggcc ggtcaggccc atcgccccca tgaccagttc gccgccgccc ggcagcgcca
     7561 ggtaacgcga cagcaccggg ccgagcgtgt agcccatgaa gccggtgaac gcgaacaccg
     7621 aggccaggcc ccaggcgctg ttgcgcagct tcatcgtcag gaacagcagg ccgaacatcc
     7681 cgaccagcat cagcagccag tgcatcggcg gcgcctgcgt gacgacggcg aaaccggcgg
     7741 tggcggcgct gaacagcagc gtcatcgaca gcagcaggta ggtgttgcgc aggacgcgat
     7801 ggccctgcac cgtggacggc gcaccggtgc gggcgccgac cggcagcggt tgatacgagt
     7861 tcatcgtcgg tgacctccgg ggatgatcga cgtggcaatg gaccggccgg acagcgaccg
     7921 gttccagcga cacattgata ttacctgtaa gaaaaaggtc tgcaagtccg gaaaccttac
     7981 gattcttaat caaggttaag aattgtctga gctgcgagtc cgaagcgcgg ctgcagaccg
     8041 atgccctgcc aggagccagc atagacggct ggtccacgta cctgatcgga agctgccatg
     8101 tcgattcacg gaagaatgca ggtcctgctg gccggcctgc tgctgattgc cagcaacggg
     8161 gcatgggcgc aggtgttcaa gtgcgtcgat gccgccggcc tgacgagcta cagccagacg
     8221 ccctgtaccg ccgatgcccg ggaagtcggc cgctggcagg gtcgggtcgg cgcggtgatg
     8281 attccggcgg gggccgcggc cgtcacgatg cgcccggtcg acgtgaaccg cacgaagctg
     8341 ccggtgcagg ctccttcgca gccgtgaggc gctgccctca gggcaggtgc gggcgggcca
     8401 gaaattcctt ggactgcatc tcctgcagcc ggctgatcgt gcgctggtac tcgaacttca
     8461 gcttctcgcc gacgtagatc gcttcgcacg gagcggccgc ggcgaggatc agcttgacgt
     8521 tgcggtcgta gaactcgtcg acgaggttca ggaagcgccg cgcggcgttt tccatggtcc
     8581 agtccagcac cggcacccgg ccgatcagca cggtgtggaa ctgccgggcg atctcgatgt
     8641 aatcgttctg cgagcgcggc ccgtcgcaga tttccgcgaa gtcgaaccag accacgccgt
     8701 cggccagttc gcgcgtgcgc aggaagcgac cctcgatctc cagccgggca ttgtgctggc
     8761 ccggctccgg ggccagggag cggaaggttt cggccagcac ggtctcggcg cgcgcatcga
     8821 gcgggtggtg gtagatctcg gcgcgctcca gatagcgcag ccggtaatcg gtgccgccat
     8881 cgacgttgac gacctcgcag tgcgtcttca gcagctcgat cgccggcagg aagcgcgcgc
     8941 gctgcaggcc gtccctgtac agcaggtcgg gcgggatgtt ggtggtcgtg accagcacca
     9001 cgccgcgctc aaacagcgcc gcgagcaggc cgtgcagcag catcgcgtcg gtgatgtcgg
     9061 cgacgaagaa ctcgtcgaga cagagcacgc gcaggttctc ggcaagctgc gcggcgacga
     9121 tcttcagcgg atcctgctgg tgtttcacgt ctttcagccg cgcatgcacc atctgcatga
     9181 agcggtggaa gtgcaggcgc tgcttctcgc gaaacggcag cgtgtcgtag aaggtgtcga
     9241 ccagataggt cttgccgcgc ccgactccgc cccagaagta caggccgcgg atcgccggcg
     9301 cggtcggcgc atcggtttcc cggctcttgc cgagcagctt gccgaacagg ccgccgccgc
     9361 tgcgcaccgg cacggccgca ggctgcggcc gggcgatcag ggcatcgtgc agcttctgca
     9421 gcaggcgcac ggcgtgttcc tgggccggat cgtgcgcgaa gcccgggcgc ttcaggtcct
     9481 gctggtaacg ggaatacggc atggcgatgg ctcggacggc agggatgcgg cagtgcggca
     9541 ttgtgcgcag ccgccagggg cagcggcaag tcaggccggg gggctgcgac gcggcagata
     9601 gcgggtccag tcgtggccgg cgtcgccgaa ggccaggaaa tccgggttga tcagcgtgtg
     9661 ccgggcgttg tagcgcagtg gccggcgtgc gccgtcgcag agcccgccgc cggcttcctc
     9721 cagcaggatc tgcgaggctg ccgtgtccca ttcgctggtc gggccgaagc gcggatagac
     9781 gtcggcctcg ccctcggcga tcaggcaggc cttcagcgcg ctgccgatgc cgcggtagac
     9841 gtgctcgccg aggtagccga gataggtctg cagcaggtgg ccggtatgcc ggcgcgagcc
     9901 ggcgacgcgc accggcccgc tgccgagcgc ccggcagcgg atcggcaccg gcgtgctgcc
     9961 cggcacgagc ttccaggcgc cgccgccgcg ccaggcccag taggccgcgc cgctgaccgg
    10021 catcaggatc agcccgaaca ccgggaggtg gtcttcgacc agcgcgatgt tgatcgagaa
    10081 ctcgccgctg cgggcgacga actcgcgcgt gccgtcgagc ggatcgacga gccacagccg
    10141 tggccagccc agccgctgcg ggtgttcgtc gggcgctgat tcctcggaca gcaccgggat
    10201 gtccggcgtc aggcgtgcaa ggccttcgca gagcacggcg tgcgcggcga ggtcggccgc
    10261 cgtgaccggc gtgcggtcgg acttcaggtg aatcgcgaag tcggaggcat agatgcgcag
    10321 gatttcgccg gcggcccgca tggccagtgc cagcaccggt tcggcgaggg cggcgagggc
    10381 ggtatccatc ggctgggccg gccgggtgct cagcgcgctg cggcgagtgc cgcgcgcgcc
    10441 agaaacagcg cggcgatcga gcgggcctcg gtgaagtcgg gccgcgcgag cagcgcatcg
    10501 agctccgaca gccgccacgg caccacctcg atcgcttcgg gctcgtcgcc ggggcgggtt
    10561 tccggataga ggtcctcggc gagcacgagg tgcgtgacgt gcgcgaagta gccgggggcg
    10621 atggtcgccg agcgcagcag cgtcagccgg cgcgcgccgt ggccgacttc ctccatgatc
    10681 tcgcggttgg cggcggcgag cgggtcttcg ccggcctcga cgacgccctt cgggcagccg
    10741 agttcgtagc ggtgcgtgcc ggcggcgtat tcgcgcacca gcaggcaggt gtcggcgtcg
    10801 agcatcggca cgatcagcac cgagccgtga ccaccgctcg gcacgcgttc gaattcgacc
    10861 tcgacaccgt tggcgaagcg caggtcgacc ttctcgaccc ggaacagacg cgagcgggcg
    10921 accgtttcgg tcgcgaggat ttcggggagc gggcgcatgg cggcgattgt cggtcgcggc
    10981 cgcgacgacg acaagcgggc tgcgcacgtc ataatcgggc ggacgccccc tgcctgcccc
    11041 cggaggcacc cgatggcaag cccgccgtgg acccgcatcg acaccgtgct gctcgacatg
    11101 gacggcacgc tgctcgacct gcgtttcgac aaccatttct ggctcgaaca cctgccggcg
    11161 cgctgggccg cgctgcacgg ccgtgagccg gccgcggccc gcgaggaact gctggcgcgc
    11221 taccgcgagg tcgagggcac gctggcctgg tactgcgtcg agcactggtc gcgggcgctc
    11281 gccgtcgacg tggtcgagct gaagcgcgag gtcgagcacc tgatccgctt cctgccggat
    11341 tcgctcgatt tcctcgcagc actgcgccag gccggcaagc gcgtcgtgct cgcgaccaac
    11401 gcccatccgt acagtctggc gctgaaggcc gagaagaccg gtctcggcgg gcatttcgac
    11461 gtgctggtca gcgcgcacga attcggcgtg ccgaaggaac acccgaaatt ctgggccagc
    11521 ctgcaggcgc gcatcggcta cgacccgccg cgcacgctgt tcctcgacga cagcctgccg
    11581 atcctgcgcg ccgcgcgtga ctacgggcac atcggccatc tggtgtcgat gctgcgcccg
    11641 gacagcgcgc agccgctgcg cgcgcgcgcc gagttcgcag ccgtgctgag gctcggcgag
    11701 ctgctgccgg tgctgccctc cggggaatga ggccgggtct gccggggccc ggaaacgcgc
    11761 gacggcgcgg gggagcgcgc cgtcgcgggt tcagacaagc cgggggcgga tggatcaggc
    11821 catcagcgca cgccgcgctt gcgggccgga gcagcggcgg tgcgcggcgg gtcggccatc
    11881 gcgatcagct cggccggtgt tgccggctga ccggcggcgg ccagccgcgc gagcgtctga
    11941 tcgggcaccc agaccgtcgc gccggccggc agcatcgccc ggccgccgag ggcggccggc
    12001 gaccagcccg ggttcagcgc cgagagctgc tcgaccgaca ggccgtagta gcttgccaga
    12061 tgcggggccg aggtcgggtg atcgagcacg acgttttcgc gcggcaccgg gatgccgcgg
    12121 gccggcgtca cgaccgtgcg tccggcgagt tcgcgggcgg ccaggaactg cgcgtagaag
    12181 ttgcgcgacg cgaagccgaa ggccttgccg tcgtagtccc agacgatgcg gccgatgtcg
    12241 gcaccgtgca ggctgcgcgc tttggccagt ccgccgacgc cgtggttgta cgaggtgatc
    12301 gcgagtcccc agtcgccgag ccggctgtag gcatcggcga gatagcgcgc cgcaccctgc
    12361 gcggccacca gcggatcgag ccgctcgtcg gtggccgtgt gcacgaccat gtagctcttc
    12421 gccgtcgagg gcatgaactg gaagatgccg gcggcgccga ccgaggagcg ggcgctggcc
    12481 tggaacgagg attcgacgtg cggcaggtag gcgagatctt cggggacgcc ggccgactgg
    12541 aagatctcgc ggaagcgctg gtcgtagcgt gcgccgatct ccaggccgcg cgcgaagcgc
    12601 tcgcggatgc cgcgctgcgc gcgcagccgc tcggcggcac cgatgacgcc ggcctcgccg
    12661 gcgctgctgg cgacgatgcg ctcgcgcagg cgcttttcat cggcgttcag cgattcgccg
    12721 gcgagctgct tgcgttcgag ttcgcgcagc tgcgcctgca ggccgtcacg acgggcgcgc
    12781 agcacggact tgtcatcggt gctcaggccc tcgcgcgcgc cgccgggcag gtcgacgatc
    12841 tcgtagacca gatcgagata gcgctcgtcg tgcagcgcgt actggccgag gctccagctg
    12901 ccgtagacct gcttccagaa ttcgaccggc gcgcgcaggt tctcgggcgc cgggaacggc
    12961 ccctggcgca cgtacgggcc gctcggcttc ggatcgacgc tggcgcaggc cgcaagcacg
    13021 cagagagccg ctgccgcgag caggcggcgg accggacggg tgtgatgcat cggaaaaccc
    13081 cctgaagatc ccctcggtcg ccggggcacc ggtgaccgcc cgaattctgg attcgtgacc
    13141 gcgatttata gaggcagcag gccggccccg gcaagccgcg ttgtacgacc gatgccgggg
    13201 gcacgcgcag gcttcagcgc ggctcgtcga ggttccagtt gccgatgtcg gcgtcgatgc
    13261 cttcaccgcc cgggtggccg tcgccgccga gcgagtagat gtcgatcgga ccgtgctggc
    13321 cggggttggc atacaggtag aaacggttcc acggatcctt cggcacctgc ggcaggtagg
    13381 ggccgtgcca gttcatcgac tcggcagcct cggccggctt gtgcgcgagg gcttcgaggc
    13441 cgacggcggt cgccgggtag cggccggtgt ccttcttgta gcggttcagg gcttcctgca
    13501 gcgtgcggat ctcggcgcgc gcggcgcgct ggcgcttctc gtcgggccgc tccatgaccc
    13561 gcggcacgat cagcgcggcg gccacgagca ggatgatcag cacggcgagc gactcgatga
    13621 gcgtgaagcc gaagctgctg ctgcggcgac gaaggcgggt gggcatgcgc ggggcggtcc
    13681 tgctggggcg tgggcggggg atcatagcaa ccgcgcgaaa cgcgcggcag cgtccgcgtg
    13741 aggaccgcgc acccggccgg gcgttcccgt catcgccggg tcatgcgcct gcaagacggg
    13801 cgcaaccgca gcgcaagcag gctgcaagaa gcactgccca gactcgccgg ccatcgtcca
    13861 gaggccgccg cggagtccgc cgccatgctt gcgcatcccg tcccgtcgcc atccatcgcc
    13921 accgaagccg tcgtcgcgcc gctggccgaa gcccggcgtg cgccacgcct gagcgtgcag
    13981 ctcgccgtaa gcaaggccga agtgcgcgaa gcgcagcggc tgcgccatgc gatcttcgtc
    14041 gaggagatgg gcgcgcgcct gcacaacacg ctgcccggcc atgacgacga cgccttcgat
    14101 ccgcactgcc ggcacctgct ggtgcgcgag acggcgggcg ggcagctgat cggctgcacg
    14161 cggctgctga ccgataccgc ggcgcgcgcc gccggcgggt tctactcgga gacggaattc
    14221 gagctggggc cggtacgcgg gctcgccgga cgcatcgccg agatcggccg cacctgcgtg
    14281 caccgcgagc accgcaacgg cgcgacgatc gcgacgctgt gggccggcat cgccggtttc
    14341 gtcgccgaac atgaggtccg gtatctgatc ggctgtgcga gcgtgccgct cgccgaggac
    14401 atggcgcagc gcgtgttctc ggagctggcc gcgcagcacc tgaccgaaga cacgctgcgc
    14461 gtgcgcccgc gccagccgct gccgcggcgt gacctcgatg cgcgcggcag ggccgtgctg
    14521 ccgccgctgc tgaaggctta tctgcgcgtc ggtgcacgca tcgccggcga gccctgcgtc
    14581 gatgcggatt tcggctgcgc cgatctgttc atcctgctcg acaccgtgca gctcgaacgc
    14641 cgctacgccc gccacttcct ggatcgtccg cagtgagcgc ggccgggctg atgcgtgtgc
    14701 tgcgccgggc ctggcggctg ccgctgctga tcctgctgct cgggctcggc tgcgcgatcg
    14761 ccgcctgcct gccgggcggc gccgggcggg tgccgtctcc ggcgccgatg gtgtggtgga
    14821 gccgccggct gtgccggctg ctcggcgtgc gcatcgtctg tcacggcacg ccgctcgccg
    14881 gccgggcgct gttcgtggcc aaccatgtgt cctggctcga catcatggtg ctcgccgcgg
    14941 tctgtccgac gcatttcctc gccaagtccg aggtggccgg ctggccgctg ttcggctggc
    15001 tgtgccggcg tgtcggcacg gccttcatcc gccgcggggc cggggacggc gcgcaggcgg
    15061 cggctgcaga actggtctgg cggctgcagc gccacgacgg ccgcgtgctg gtattccccg
    15121 agggcacctc gaccgacggc cgcggcgtgc gccgcttcca tgcgcggctg ctgcaggcgg
    15181 ccgagcgcgc gcagtgcccg gtgcaggcgc tggcgctgcg ctatccggtc gccgggggcg
    15241 gcacgcatcc ggccgtgccc ttcatcggcg acgacgagct gctcgcgcat ctgtggcggc
    15301 tgctcggcga gcagcgcatc gaggccgagg tgagctgcct cgcgccgctg ccggccggca
    15361 tcggccgcga cgcgctggcc cggcgcagcc acgcgcagat cgccgcggtg ctggccggcg
    15421 acgatccgca gccccggcgc gccatcggct gaaatccgct cgccggcttc aggcgccggc
    15481 gagcgtcacg tacacgtcgt agcgcgtgtt cgggcttgcg atcacatgcg agggcgcccg
    15541 cccggccagc ggcggcgcgc ccttcggccg cttgacgacg acgcggcgca gcgcgaccgc
    15601 cagcgccggg gcgagcagcg catcggcatc caggtcgtcg ccgaccacgg cgcggaaggc
    15661 gcgcatctcc ttcttcacca gcgccgactg ttcacggtgc ggatacatcg ggtcgaggta
    15721 gatcacctcg ggccgctgcg ccggcgcggc ctgcgcttgc tgcgcggctg cctgcagcca
    15781 ggcgctgccg tcggcgtgga ccagctgcac gcgctcgcgc acccaggaac cgatgccggt
    15841 gtcggcggcg gcgcgggcga tgccgtcatc gagcagcgcg cacaccaccg ggctgcgctc
    15901 gaccagcgtc acccggcagc cgagcgcggc gagcacgcag gcatcgcgcc cgagtccggc
    15961 ggtcaggtcg gcgaccgccg gacaggcgcc gcccttgagt
//
LOCUS       DAOQIE010000045        15972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1729_length_15972_cov_7.682164, whole genome shotgun sequence.
ACCESSION   DAOQIE010000045 DAOQIE010000000
VERSION     DAOQIE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1729_length_15972_cov_7.682164"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK144_04570"
     CDS             complement(<1..496)
                     /locus_tag="PK144_04570"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNF65035.1"
                     /translation="MAQAPASFRDFTLQSRWQPAARLRRAEMGERLRDCLLGRGSLTA
                     RLRAECARHGRAFSVRVLSERAGRVLREEADALGLRRGVTAWVREVHLLAGGVPWVFA
                     RTVIPRRTLRGHGRTLTGLGTRPLGAVLFADPGVVRGPLQIARLDLRCPLARRALDVR
                     GASGL"
     gene            complement(647..1720)
                     /locus_tag="PK144_04575"
     CDS             complement(647..1720)
                     /locus_tag="PK144_04575"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNF65036.1"
                     /translation="MPLVAHSGLPSFGRLAEEGEEILAQARAAQQDIRELHIGLLNMM
                     PDAALAATERQFMRLIGSSNRIAQFYVHPFTVGGIGRGAEAEAYIGRWYESFDTIRAQ
                     GLDALIITGANISNPDFTQEPFWGPLSEVVEWAHEQVTTTLFACLAAHATMLMKYGVH
                     RRRLPDKAWGVFPHRVVDRRHPLVRNINTRFDVPHSRWNEITREQLDDAGLVTLVESA
                     EVGPHLVVSPDGFRQVFFQGHPEYDIDSLLKEYKREVLRYLHGERADYPPLVQHYFPE
                     AAQTLLYEYKNHVFQQQDHGRQPDGSDFPEAAVLRLLDNTWADTAKAIFNNWLGLMYQ
                     ITGHERAVPFMPGVDPDDPLNGL"
     gene            complement(1814..3916)
                     /gene="recG"
                     /locus_tag="PK144_04580"
     CDS             complement(1814..3916)
                     /gene="recG"
                     /locus_tag="PK144_04580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNF65037.1"
                     /translation="MTHPLAEAGRGVSASRSLDALKGVGPKLAGRLAKLGLRTVEDLL
                     FHLPLRWQDRTQVVPISELTIGEPAVIEAEVLGMETVGRTRQSFLVRVGDGSGTLVLR
                     FFHFGASLQAAMARGARLRLFGEVRSGPTGPEIIHPEHRPADAPADDEATLTPVYPST
                     EGLHQLALRKITAEALRLLDAVPDLLPAAIAREHGFPDLHAALRLLHRPPPGVELEAL
                     ALGTHPAQQRLAFEELIAHQLALRELRRRLQCLQAPKLAAGALRARWLAALPFALTGA
                     QQRALADIDADLQGSRPMLRLVQGDVGCGKTVVAAAAALSAVEAGMQAAVMAPTELLA
                     EQHLRNFTQWLEPLGVRVEWLVGRLKGRGREAALAAIADGSAQVVVGTHALFQEGVNF
                     ARLALVIIDEQHRFGVHQRLQLRDKGREADRDGSRCPHQMVMTATPIPRTLAMSAYAD
                     LDTTVIDELPPGRTPVTTVAVPEARRAEVLERVRAACRGGRQAYWVCTLVEESETLDA
                     QAATDIAAALMQQLPELRIGLVHGRLRGVDKEAVMARFKAGELDLLVATTVIEVGVDV
                     PNASLMIIENAERLGLAQLHQLRGRVGRGAAASSCVLMYHPPLSEQGRARLAVLRETS
                     DGFVIARRDLELRGPGEVLGTRQTGTLALRIADLLRDEPLLPAVQRAADRLLVEHPNA
                     AAALARRWTGERERYGEV"
     gene            complement(3941..4321)
                     /locus_tag="PK144_04585"
     CDS             complement(3941..4321)
                     /locus_tag="PK144_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016968788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF65038.1"
                     /translation="MAESLIEYEDACEEVRAVYDDIMRTRKVEHVNNFWKAIAHHPPT
                     LARTWEAMKAVMADDGGLDPLMKELIYIAVSVANGCDYCIASHTAAARRKGMTEAMHG
                     ELLAVIGMASATNALATAHRVPID"
     gene            complement(4461..5207)
                     /locus_tag="PK144_04590"
     CDS             complement(4461..5207)
                     /locus_tag="PK144_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HNF65039.1"
                     /translation="MSSVPAVSALLPAVAGRLLCAAVLAATAACSGPGSGQAVYKNEQ
                     FAASSDYSHRYAAPPVATCEAARRALLSQGYVIVDAKPEAIEARKTFQPDRENHTELN
                     FRITCAGDGDVDSDGFADAALLFANAEQTLYTVKKTSSSASVGVGVVGSISLPIGQSQ
                     DSLIKIGAETVPAGPFYQRFFALVQQYLGSSRPKPRPAPVTPPVAPPPPAAPAALPPV
                     PAPALPPGSAPLPPVPPLPAPASAAPLTPT"
     gene            complement(5234..6586)
                     /locus_tag="PK144_04595"
     CDS             complement(5234..6586)
                     /locus_tag="PK144_04595"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNF65040.1"
                     /translation="MSSPWTPDSWRRHPAQQMPEYADAAALAAAEAQLAKYPPLVFAG
                     EARTLKKSLAEVAEGRAFVLQGGDCAESFAEFHAFNIRDTFRVLLQMAVVLTYGAGLP
                     IVKLGRMAGQFAKPRSAPVETRGDVTLPSYRGDNVNGLEFTAAARAPDPQRQVQAYFQ
                     SAATMNLLRAFAQGGYADLHQVNRWTADFIAASAQGDRYRELADHIGRTLKFMEACGL
                     NGDNTPQIRETAFYTSHEALLLGYEQALTRRDSLTGDWYDCSAHLLWIGERTRQPDGA
                     HIEFCRGIGNPVGVKVGPKTKPGDVLRLCDLLNPANEAGRLMLIARMGADTVGDALPA
                     LVRAVQAEGRKVVWSCDPMHGNTITSSTGYKTRPFERVLAEVRGFFAVHRAEGTHAGG
                     VHFEMTGQNVTECTGGAEGIDDEALAACYLTHCDPRLNGSQSLELAFLIAEMLKAERA
                     "
     gene            6791..7216
                     /gene="dksA"
                     /locus_tag="PK144_04600"
     CDS             6791..7216
                     /gene="dksA"
                     /locus_tag="PK144_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNF65041.1"
                     /translation="MTNELQLPGNYRPSADEPYMNERQLEYFRRKLLAWREELLAEAQ
                     STIDAMREAPTDGADEADRASRESDTSFELRTRDRYRKLLNKIESALKRIADGSYGYC
                     DETGEEIGLGRLEARPIATLTVEAQERRERRQSLQADPH"
     gene            7548..10352
                     /locus_tag="PK144_04605"
     CDS             7548..10352
                     /locus_tag="PK144_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65042.1"
                     /translation="MNPALRTAEGHYTLAALDAFAWMLTPVWVFDLTVPTILWANAAA
                     LELWEARDLAELQARDFSDLTDTTRQRLARALAEIRDGRTCAEQWTFYPKGRPVHLIV
                     NLTGIVLSDGRLTTLHEARPVVPEQPDPLALRGIEALTHTPVMITLHDAEGRVLMQNP
                     AAQRSFGIADAAREQLRARCADPLDAASLLDALEPEAGWVGEIPRLTTAGLRWHRVKA
                     HRTTDPVTGAAAVLVSEHDVHELHEARTRLAHSEARLRSIVEGVAAVLWEVELPSGRL
                     SYVSPQIEQLSGYPSTEWYDPQVWLARIDAEDREHVRCSLSEQLAAGRDGTLEYRLQR
                     ADGSLAWWRTHFRVQRGLDGRPLRAIGFLLDISDRVATEERLRVAELVFDHADQGIFV
                     TDAEQRIRYVNPAFETISGYGAGELLGQTPRLLRSGHQDERFYAEMWSTLRRDGRWRG
                     ELWNRRRNGDLYAQQLTIRAVPDPHGRSTRYLALASDVTEQRQRADRLQHLALHDALT
                     GLPNRKLLNDRLAQAVRLGRRESLGFALIALDLDGFKGVNDTWGHAAGDRLLRSAGRR
                     LTNAVRASDTVARLGGDEFSVLLWDIAGRHDAEAVAAKLLAALRRSFSIDGHTVQIGA
                     SFGVALYPQDGADAATLTVHADAALYRAKSAGRNLYAFYTPTLEHEAHERNRLEGALR
                     RLLDGGALAVAIQPEVDLADGRITGAELLARWPDADTEAPVPPARFIAVAEECGLIAA
                     LSEAVLQRALGLLRAWQPWLPRGFRLSVNLSPALLRQGEAQPLCRRVLAGEPALAAAL
                     MLDLPERGLFAEDAATRRRLHALRALGPGLALDDFGQGTLPLAGLREGDFDVLKITRA
                     LVAGLDAEAAQSGLAAALLGLGQALGLPVIAKGVETAAQARALRALGCTRALGFHFAP
                     PLTPEAFSGLLAGGHKLPPS"
     gene            complement(10367..12460)
                     /gene="spoT"
                     /locus_tag="PK144_04610"
     CDS             complement(10367..12460)
                     /gene="spoT"
                     /locus_tag="PK144_04610"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HNF65043.1"
                     /translation="MVLAGYLDPAQVEAVRSACWFSAEAHQGQYRKSGEPYVFHPIAV
                     ARILAEVHFDHETLMAAVLHDVIEDTKFGKDQVTERFGPEVAELVDGVTKLTQIQFGS
                     KLEAQAENFRKMFLAMAKDIRVIMVKLGDRLHNMRTLGAMPAEKRRRIARETLEIYAP
                     IAGRLGMNHLRLELENLGFAYLYPTRHRVLSEAVRKLRGNRREIISQIETRVRNRLLE
                     EGIKARVVGREKHLWGIYKKMHEKRLGLKEVYDVYAVRLIVDSVDTCYRTLGLVHGLY
                     KPIMGRFKDYVAIPKANGYQSLHTVLFGPHGVPIEVQIRTQDMHVMAEAGVAAHWLYK
                     SPGAQGNAAHARAREWLARLLDMQRRAGNSVEFLESVKMDLFPDEVYVFTPRGEIIEL
                     PKGATAVDFAYAIHSDVGNTCVAAKVDRRLVPLRTPLANGQTVEVIITPTARPNPAWL
                     GFVVTAKARASIRHYLKNLQRDEAVLLGKRLLEKSLVGQVGSLADLPQARVQALTKEL
                     GVAGFDEILADIGLGTRMAPLIAKRLLPGGESAEDRPRAGQGAPLLIKGTEGTVVSFG
                     KCCRPLPGDAIIGYLTAGRGVVVHRENCRNVADYKKNPEKWIEIEWAESLRSDFPVDV
                     RIDVSSKRGVFASVAAAISAADANIETISTSERDGVTATVNLTLNVRDRVHLAQVIKR
                     LRALPEVLRIARRGQ"
     gene            complement(12593..12928)
                     /gene="rpoZ"
                     /locus_tag="PK144_04615"
     CDS             complement(12593..12928)
                     /gene="rpoZ"
                     /locus_tag="PK144_04615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNF65044.1"
                     /translation="MARVTVEDCLDRVENRFQLVLVASRRARQIALGQMPLVPEENDK
                     PTVIALRELAAGLIDPQALMREPQVLPEREREPVLADFSFDEPLDMAGGEPSPVTPPA
                     PGLDGEANA"
     gene            complement(13072..13689)
                     /gene="gmk"
                     /locus_tag="PK144_04620"
     CDS             complement(13072..13689)
                     /gene="gmk"
                     /locus_tag="PK144_04620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNF65045.1"
                     /translation="MTASGQLYIVSAPSGAGKTSLVRALLTQDVQVEVSVSHTTRAPR
                     PGERDGEHYHFVSDDAFARLVADDAFLEHAQVFDRRYGTARTGVAARLAAGRDVILEI
                     DWQGARQVRAAFPEAQSVFIVPPSVGELRRRLSGRGQDAAEVIERRMRDARSELMHWV
                     EFDYLIVNDDFGRALAELQALFTARRLRRSVQAERHGALLDALLD"
     gene            complement(13733..14599)
                     /locus_tag="PK144_04625"
     CDS             complement(13733..14599)
                     /locus_tag="PK144_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNF65046.1"
                     /translation="MIRSMTAFARCERHTEPGAIAWELRTVNHRYLEISLRMPETLRA
                     LDLAVRERVQGRLTRGKVDASLRFQPATSSAPELTLNRAYAERLLGIAGELENLIGPG
                     TGLRLADVLRWPGVVTEAEQDLEPLKRGVLEVLDAALAELVATREREGARTAEMILSR
                     CDAIAAQVAFVRQRRPEVVARLREKLTGRLADLPAGADPGRLEQEMVIVAQRMDVEEE
                     LDRLDAHLAEIRDVLRRPEPVGRRLDFLMQELNREANTLSSKSLDTETTRAAVELKVL
                     IEQMREQIQNIE"
     gene            14771..15493
                     /gene="rph"
                     /locus_tag="PK144_04630"
     CDS             14771..15493
                     /gene="rph"
                     /locus_tag="PK144_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNF65047.1"
                     /translation="MRPSGRAPEQLRPIRLTRQFTRHAEGSVLVEFGETRVICTASVE
                     ERVPPFLKGKGQGWITAEYGMLPRATHTRSPREAARGAQSGRTQEIQRLIGRSLRACA
                     DLAALGERTITIDCDVIQADGGTRTAAITGGFVALADAVRTLLERRSLKKNPLHGQIA
                     SVSVGIWRGTPVLDLDYAEDSEAETDMNVVMNDAGAYIEVQGTAEGHAFRAEELDAML
                     ALARAGIAELIGHQRAALGIGR"
     gene            complement(15498..>15972)
                     /locus_tag="PK144_04635"
     CDS             complement(15498..>15972)
                     /locus_tag="PK144_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65048.1"
                     /translation="GPVGGRLGAGAARQMERLNGARDADGWRAALGELDAALPRLVGR
                     RDLERVRTALAGLVAVQVEAASVPVTGLPAALDQVRRRTLALLAESVGPVGGRMNAAA
                     ARRIERLEAVTTPEDLHAALSELESALPRLVARTELDRVRVTLGRLRRTAERAEG"
BASE COUNT         2211 a         5766 c         5711 g         2284 t
ORIGIN      
        1 gcaggccgct ggcaccccgc acgtcgagcg cgcggcgcgc cagcggacag cgcaggtcga
       61 ggcgcgcgat ctgcagcggg ccgcgcacga cgccgggatc ggcgaacagt accgcgccga
      121 gcggacgcgt gccgagcccg gtcagcgtgc ggccgtggcc gcgcagcgtg cgccgcggga
      181 tcacggtgcg cgcgaacacc cacggcacac cgccggcgag caggtgcacc tcgcgcaccc
      241 aggcggtgac gccacggcgc aggccgagcg catcggcctc ctcgcgcagc acgcgcccgg
      301 cgcgctcgct cagcacccgc accgagaagg cccggccgtg gcgcgcgcat tcggcgcgca
      361 gccgtgcggt cagcgaaccg cggccgagca ggcagtcgcg cagccgctcg cccatctcgg
      421 cgcgccgcag ccgcgcagcc ggctgccagc gcgactgcag cgtgaagtcc cggaagctgg
      481 cgggcgcctg cgccacggcg cgaggcccgg aagcggcagg accgccgggg accggcactt
      541 gcgtcagcag ccgcgacggc cgcaccggcg ccggggccgc accgcccgcc cgccgcccgt
      601 ccgtcgtctc ccctgccctc acgccgcccg tctcccgcag catccctcag agtccgttca
      661 gcggatcgtc cggatcgacg ccgggcatga acggcacggc ccgctcgtgg ccggtgatct
      721 ggtacatcag gccgagccag ttgttgaaga tcgccttggc ggtgtcggcc caggtgttgt
      781 cgagcaggcg cagcaccgcg gcttccggga agtcgctgcc gtcgggctgg cggccgtggt
      841 cctgctgctg gaagacgtgg ttcttgtact cgtagagcag cgtctgcgcg gcctcgggga
      901 agtaatgctg caccagcggc ggatagtcgg cgcgttcgcc gtgcaggtag cgcagcacct
      961 cgcgcttgta ttccttcagc aggctgtcga tgtcgtactc cggatgcccc tggaagaaca
     1021 cctgccggaa gccgtcgggg ctgaccacca gatgcgggcc gacctcggcg ctctcgacca
     1081 gcgtgaccag cccggcgtca tcgagctgct cgcgggtgat ctcgttccag cgcgagtgcg
     1141 gcacgtcgaa gcgcgtgttg atgttgcgca ccagcgggtg ccggcgatcg acgacgcggt
     1201 gcggaaacac gccccaggcc ttgtccggca gccggcggcg gtgcacgccg tacttcatca
     1261 gcatggtcgc gtgcgcggcc agacacgcga acagcgtggt cgtgacctgc tcgtgcgccc
     1321 attcgaccac ctcggagagc ggcccccaga acggttcctg cgtgaagtcg gggttggaga
     1381 tgttggcgcc ggtgatgatc agcgcatcca ggccctgcgc ccggatggtg tcgaaggact
     1441 cgtaccagcg cccgatgtag gcctcggcct cggcgccgcg cccgatgccg ccgaccgtga
     1501 acggatgcac gtagaactgc gcgatgcggt tgctcgaacc gatcaggcgc atgaactggc
     1561 gctcggtcgc ggcgagcgcg gcgtccggca tcatgttcag caggccgatg tgcagttcgc
     1621 ggatgtcctg ctgcgcggcg cgcgcctgcg cgaggatttc ctcgccttcc tcggcgagcc
     1681 gcccgaagct gggcaggccg gaatgggcaa cgagtggcat ggtgatgcgg tctctgcgga
     1741 tcgtgggttc ggctgcacgg cggatcgcag cgcagccgcc ggttgtgaca ggggcgcgca
     1801 ggatgggtgc ggttcagacc tcgccgtagc gctcgcgctc gccggtccag cgacgggcca
     1861 gcgcggcggc ggcgttcggg tgttcgacga gcaggcggtc ggcggcgcgc tgcacggccg
     1921 gcagcagggg ctcgtcgcgc agcaggtcgg cgatgcgcag cgccagcgtg ccggtctggc
     1981 gggtgccgag cacctcgccg gggccgcgca gttcgaggtc gcgccgcgcg atcacgaagc
     2041 cgtcactggt ttcgcgcagc acggcgagcc gcgcccggcc ctgctcggac agcggcggat
     2101 ggtacatcag cacgcagctc gatgccgcag ccccccgccc gacccggccg cgcagctggt
     2161 gcagctgcgc aagccccaga cgctcggcgt tctcgatgat catcaggctg gcgttcggca
     2221 cgtcgacgcc gacctcgatg accgtggtcg cgaccagcag gtcgagctcc ccggccttga
     2281 agcgcgccat cacggcttcc ttgtcgacgc ccctgagccg cccgtgcacg aggccgatcc
     2341 gcagttccgg cagctgctgc atcagggcgg cggcgatgtc ggtcgccgcc tgcgcgtcca
     2401 gggtttccga ctcctcgacc agcgtgcaca cccagtaggc ctgccggccg ccgcggcagg
     2461 cggcgcgcac gcgttcgagc acctcggcgc ggcgcgcttc cggcaccgcg accgtcgtca
     2521 ccggcgtgcg cccgggcggc agctcgtcga tgaccgtggt gtcgaggtcg gcataggcgc
     2581 tcatcgccag cgtgcgcggg atcggcgtcg cggtcatgac catctggtgc gggcagcggc
     2641 tgccgtcgcg gtccgcctcg cggcccttgt cgcgcagctg caggcgctgg tgcacgccga
     2701 agcggtgctg ctcgtcgatg atcaccagcg ccaggcgcgc gaagttcacg ccctcctgga
     2761 acagcgcgtg cgtgccgacg acgacctggg ccgagccatc ggcgatcgcc gcgagtgcgg
     2821 cctcgcgccc gcggcccttg aggcgcccga ccagccattc gacgcgcacg ccgagcggct
     2881 ccagccactg cgtgaagttg cgcaggtgct gctcggcgag cagctcggtc ggcgccatca
     2941 ccgccgcctg catgccggcc tcgaccgccg agagcgccgc cgcggccgcg accacggtct
     3001 tgccgcagcc gacatcgccc tgcaccaggc gcagcatcgg ccggctgccc tgcagatcgg
     3061 cgtcgatatc ggcgagcgcg cgctgctgcg cgccagtcag cgcaaacggc agcgccgcga
     3121 gccagcgcgc gcgcagcgcc ccggccgcga gcttcggcgc ctgcaggcac tgcaggcgcc
     3181 ggcgcagctc gcgcagcgcg agctggtggg cgatcagttc ctcgaaggcc agacgctgct
     3241 gcgccgggtg cgtgccgagg gcgagcgctt cgagctcgac gcccggcggc ggccggtgca
     3301 gcagacgcag cgccgcgtgc agatccggaa agccatgctc gcgcgcgatc gccgccggca
     3361 gcaggtcggg cacggcatcg agcaggcgca gcgcctcggc ggtgatcttg cgcagcgcaa
     3421 gctgatgcag gccctcggtg ctcgggtaga ccggcgtcag cgtcgcctcg tcgtcggccg
     3481 gcgcatcggc cgggcggtgc tcgggatgga tgatttccgg gccggtcggg ccgctgcgca
     3541 cctcgccgaa cagccgcagc cgcgcgccgc gcgccatcgc cgcctgcagc gaggcaccga
     3601 agtggaagaa gcgcagcacc agcgtgccgc tgccatcacc gacgcgcacc aggaaggact
     3661 ggcgcgtgcg cccgaccgtc tccatgccga gcacctcggc ctcgatcacg gccggctcgc
     3721 cgatcgtgag ttcgctgatc ggcacgacct gcgtgcggtc ctgccagcgc agcggcaggt
     3781 gaaacagcag atcctcgacc gtgcgcaggc cgagcttcgc cagccgcccg gcgagcttgg
     3841 ggccgacgcc cttcagcgcg tccagcgagc ggctggcgga gacgccgcga ccggcctcgg
     3901 cgagcggatg cgtcatgcgg gcgcgatccc gaggaccggc tcagtcgatc ggcacgcggt
     3961 gcgcggtggc gagcgcgttg gtggcgctcg ccatgccgat caccgccagc agctcgccgt
     4021 gcatggcctc ggtcatgccc ttgcgccgtg ccgccgccgt gtgcgaggcg atgcagtagt
     4081 cgcagccgtt ggcgacgctg accgcgatgt agatcagttc cttcatcagc ggatcgagcc
     4141 cgccgtcatc ggccatcacc gccttcatgg cctcccaggt gcgcgccagc gtcggcggat
     4201 ggtgcgcgat cgccttccag aagttgttga cgtgctcgac cttgcgcgtg cgcatgatgt
     4261 cgtcgtagac ggcacgcacc tcctcgcagg cgtcctcgta ctcgatcagg gattcggcca
     4321 tggttccggt cctcgggctg cacgggggcc ggccgcgcag gcgcggcggc cgggacgggc
     4381 gatgcggccg gacgccctgc cccgggcgcc cgctcatcga tcatgcaccg ccggcagcgc
     4441 aacggtcagc cccgctgcgc tcaggtcggc gtcagcggtg cggcgctggc cggcgcgggc
     4501 agcggcggta ccggaggcag cggcgccgat cccggcggca gggccggtgc cgggaccggc
     4561 ggaagcgcgg ccggcgcggc cggcggaggt ggtgcgaccg gcggcgtcac cggcgccggt
     4621 cgcggcttcg ggcggctgct gccgagatac tgctgcacca gcgcgaagaa gcgctggtag
     4681 aacggcccgg ccggcacggt ctcggcaccg atcttgatca gcgaatcctg actctggccg
     4741 atcggcagcg agatcgaacc gaccacgccg acgccgacgc tggccgagga gctggtcttc
     4801 ttgaccgtgt acagcgtctg ctcggcgttc gcgaacagca gcgcggcatc ggcgaagcca
     4861 tcgctgtcga catcgccgtc gccggcgcag gtgatgcgga agttcagctc ggtgtggttc
     4921 tcgcgatcgg gctggaaggt cttgcgtgcc tcgatcgctt cgggcttcgc atcgacgatc
     4981 acgtaaccct ggctgagcag cgcgcggcgc gcggcctcgc aggtcgcgac cggaggtgcc
     5041 gcgtagcggt gcgaatagtc ggacgaggcc gcgaactgtt cgttcttgta gacggcctgg
     5101 ccgctgccgg gcccgctgca ggccgcggtg gcggcgagca cggcggcgca gagaagccgg
     5161 ccggcgacgg ccggcagcag cgcggatacg gcgggtacgg acgacatcgg ctcctcaccg
     5221 gctccgggcg cgctcacgcg cgttcggcct tcagcatctc ggcgatcagg aatgcgagtt
     5281 ccagcgactg cgagccgttc aggcgcggat cgcagtgcgt cagatagcag gcggccagcg
     5341 cctcgtcgtc gatgccttcg gcgccaccgg tgcattcggt gacgttctgc ccggtcatct
     5401 cgaaatgcac gccgccggcg tgcgtgccct cggcgcggtg cacggcgaag aagccgcgca
     5461 cctcggcgag cacgcgctcg aacggccggg tcttgtagcc ggtgctcgac gtgatcgtgt
     5521 tgccgtgcat cgggtcgcag gaccagacca ccttgcgccc ttccgcctgc accgcgcgca
     5581 ccagcgccgg cagcgcgtcg ccgaccgtgt cggcgcccat gcgcgcgatc agcatcagcc
     5641 ggccggcctc gttggcgggg ttgagcaggt cgcagaggcg cagcacgtcg ccgggcttcg
     5701 tcttcgggcc gaccttgacg ccgaccgggt tgccgatgcc gcggcagaac tcgatgtgcg
     5761 cgccatcggg ctggcgcgtg cgttcgccga tccacagcag gtgcgccgag cagtcgtacc
     5821 agtcgccggt caggctgtcg cgccgggtca gcgcctgctc gtagccgagc agcagggcct
     5881 cgtgcgaggt gtagaacgcg gtctcgcgga tctgcggcgt gttgtcaccg ttcaggccgc
     5941 aggcctccat gaacttcagc gtgcggccga tgtgatcggc gagctcgcgg tagcggtcgc
     6001 cctgcgcact ggcggcgatg aagtccgccg tccagcggtt gacctggtgc aggtcggcat
     6061 agccgccctg cgcgaaggcg cgcagcaggt tcatggtcgc ggccgactgg aagtaggcct
     6121 gcacctgacg ctgcgggtcc ggcgcgcgcg ctgcggccgt gaattcgagg ccgttgacgt
     6181 tgtcgccgcg gtagctcggc agcgtcacgt cgccgcgcgt ctcgaccggc gcgctgcgcg
     6241 gcttcgcgaa ctgcccggcc atgcgcccga gcttcacgat cggcaggccg gcgccgtagg
     6301 tcagcaccac ggccatctgc agcagcacgc ggaaggtgtc gcggatgttg aaggcgtgga
     6361 actcggcgaa gctctcggca cagtcaccgc cctgcagcac gaaggcgcgc ccctcggcga
     6421 cctcggcgag cgacttcttc agcgtgcgcg cctcgccggc gaacaccagc ggcgggtact
     6481 tcgcaagctg cgcctcggcg gcggcgagcg cggcggcatc ggcgtattcg ggcatctgct
     6541 gcgccggatg ccggcgccag ctgtcagggg tccacgggct gctcacgcta ctcatgctgc
     6601 tcacgccatt catgccactc atccacgtcg tctcgccggt acgcaggccg gcacgggcag
     6661 ggccggagct gcaaacaagc cggcaagcct cgcccatcct tgtcaatccc gcaagcgatg
     6721 gtcatatcat gcggtctctg gcgcctgccc tgccggcccg cctgcgccag cccacaggag
     6781 cccataacta atgaccaacg aactgcaact gccgggcaat tatcgtccga gcgccgacga
     6841 gccctacatg aacgagcgcc agctcgaata cttccggcgc aagctgcttg cctggcgtga
     6901 ggaactgctc gccgaggcgc agtcgacgat cgacgcgatg cgcgaggctc cgaccgatgg
     6961 cgccgacgag gccgaccgtg ccagccgcga gagcgacacc agcttcgagc tgcgcacgcg
     7021 cgatcgctac cgcaagctgc tgaacaagat cgaatcagca ctgaagcgca tcgccgacgg
     7081 cagctacggc tactgcgacg agaccggcga ggagatcggt ctcggccggc tcgaagcccg
     7141 gccgatcgcg acgctgacgg tcgaggcgca ggagcgccgc gagcgtcgcc agtcgctgca
     7201 ggccgacccg cactgaacgc cggacccggg gacggctggc tgacgctgcg cgaactcgac
     7261 gcccgccacg gcgccggcaa gggcacggcg ttccgggcgt ttcgcgccgc gctgcccggc
     7321 ctcgtcgaag gccgtgactt ccgccggctc gatgccgtcg ccgatgcggc cgagatcgcc
     7381 gtcctgcgcg ctggcgggcg gacctacgcc tccagcgtcc acgtcgtgct gctcagtccg
     7441 gcgacggccg aacggctgct gacgcactga aagcccgtgc ccggtgcaca tccgctgcga
     7501 gcggattatc gtgtgcggtc gtccccgtcc tgcccggatc gcccgccgtg aaccccgcct
     7561 tgcgcaccgc cgaaggccat tacacgctgg ccgcgctcga tgcattcgcc tggatgctga
     7621 cgccggtctg ggtgttcgac ctgacggtgc cgaccatcct gtgggccaac gccgcggcgc
     7681 tcgaactctg ggaggcacgc gatctcgccg agctgcaggc gcgcgacttc tccgatctca
     7741 ccgacacgac gcgccagcgc ctggcccgcg cgctggccga aatccgcgac gggcgcacct
     7801 gcgccgagca gtggaccttc tatccgaagg gccgcccggt gcacctgatc gtcaacctga
     7861 ccggcatcgt gctctccgac ggccggctga cgacgctgca cgaggcccgg ccggtcgtgc
     7921 ccgaacagcc cgatccgctg gcgctgcgcg gcatcgaggc cctcacgcac acgccggtga
     7981 tgatcacgct gcacgacgca gaaggccgcg tgctgatgca gaacccggcc gcgcagcgca
     8041 gcttcggcat cgccgatgcg gcccgcgagc agttgcgcgc gcgctgcgcc gacccgctcg
     8101 acgccgcctc gctgctggac gcactcgaac ccgaagccgg ctgggtcggg gaaatcccgc
     8161 gcctgaccac cgccggcctg cgctggcatc gcgtcaaggc gcaccgcacg accgacccgg
     8221 tcaccggggc ggcggccgtg ctggtctccg agcacgacgt gcatgagctg cacgaggcgc
     8281 gcacgcggct cgcgcacagc gaggcgcggc tgcgctcgat cgtcgaaggc gtggccgccg
     8341 tgctctggga ggtcgagctg ccgtccgggc ggctgtcata cgtgtcgccg cagatcgagc
     8401 agctctcggg ctatccgagc accgagtggt atgacccgca ggtctggctc gcgcgcatcg
     8461 acgccgagga ccgcgagcac gtgcgctgca gcctgagcga acagctggcg gccgggcgcg
     8521 acggcacgct cgaataccgc ctgcagcgcg ccgacggcag cctcgcgtgg tggcgcacgc
     8581 actttcgcgt gcagcgcggc ctcgacggcc ggccgctgcg ggcgatcggc ttcctgctcg
     8641 acatcagcga ccgcgtcgcc accgaggagc ggctgcgcgt cgccgaactg gtgttcgatc
     8701 acgccgatca gggcatcttc gtcaccgacg ccgagcagcg catccgctac gtgaatccgg
     8761 ccttcgagac gatctccggc tacggcgccg gcgaactgct cgggcagacg ccgcggctgc
     8821 tgcgctccgg ccatcaggac gaacgctttt atgccgagat gtggagcacg ctgcgccgcg
     8881 acggccgctg gcgcggcgag ctctggaacc ggcgccggaa cggtgacctg tacgcccagc
     8941 agctgacgat ccgcgccgtg cccgatccgc acggccgttc gacgcgctat ctggccctgg
     9001 cctcggacgt gaccgagcag cgccagcgcg ccgatcgcct gcagcacctg gcgctgcacg
     9061 atgcgctgac cgggctgccg aaccgcaagc tgctgaacga ccggctggcg caggcggtgc
     9121 gcctcggccg gcgcgaatcg ctcggtttcg cgctgatcgc gctcgatctc gatggcttca
     9181 agggcgtcaa cgatacctgg ggccacgccg ccggcgaccg cctgctgcgc tcggccggcc
     9241 ggcggctcac gaacgccgtg cgcgcctccg acaccgtcgc gcggctcggc ggcgacgagt
     9301 tttcggtgct gctgtgggac atcgccggcc gccacgacgc cgaggccgtc gccgcgaagc
     9361 tgctcgcggc gctgcggcgc agtttctcga tcgacggcca cacggtgcag atcggcgcca
     9421 gcttcggcgt cgcgctgtat ccgcaggacg gcgccgatgc cgcgacgctg acggtgcatg
     9481 ccgatgccgc gctgtaccgc gccaagtccg ccggccgcaa cctgtacgcg ttctacacgc
     9541 cgacgctgga gcacgaggcg cacgagcgca accggctcga aggggcgctg cgccgcctgc
     9601 tcgatggcgg tgcgctggcg gtcgccatcc agccggaggt cgatctcgcc gatggccgca
     9661 tcaccggcgc cgaactgctc gcccgctggc ccgacgctga caccgaagcg ccggtgcccc
     9721 cggcgcgctt cattgcggtg gccgaggaat gcgggctgat cgccgcgctc agcgaagccg
     9781 tgctgcagcg cgccctcgga ctgctccgcg cctggcagcc gtggctgccg cgcgggtttc
     9841 gcctgagcgt caacctgtca ccggcgctgc tgcgtcaggg cgaggcgcag cccctgtgcc
     9901 ggcgcgtgct cgccggcgag ccggcgctgg ccgcggcgct gatgctcgat ctgcccgaac
     9961 gcggcctgtt cgccgaggac gccgcgaccc ggcgccggct gcacgcgctg cgcgcgctgg
    10021 gccccggact cgcgctcgat gacttcggtc agggcacgct gccgctcgcg ggcctgcgcg
    10081 aaggtgactt cgacgtgctg aagatcacgc gggcgctggt ggccggactc gatgcggagg
    10141 ctgcgcagtc ggggctggcg gccgccctgc tcggccttgg tcaggccctc ggcctgccgg
    10201 tgatcgccaa gggcgtcgag accgcagcgc aggcccgcgc gctgcgcgcg ctgggctgca
    10261 cgcgggccct cggctttcac ttcgccccgc ccctgacgcc ggaggcgttc tccggactgc
    10321 tcgccggcgg acacaaactg ccgccctcct gagcggcgcg cgccgctcac tggccgcgcc
    10381 gggcgatcct cagcacttcc ggcagcgcgc gcaggcgctt gatgacctgc gcaagatgca
    10441 cgcggtcgcg cacgttcagc gtcaggttga cggtcgccgt gacgccgtcg cgctcgctgg
    10501 tcgagatggt ctcgatgttg gcgtcggccg ccgagatcgc cgcggcgacg ctcgcgaaca
    10561 cgccgcgctt gctggagaca tcgatgcgca cgtcgaccgg gaaatcactg cgcaacgact
    10621 cggcccactc gatctcgatc cacttttccg gattcttctt gtagtcggcg acgttgcggc
    10681 agttctcgcg atgcacgacc acgccgcgcc cggcggtcag gtagccgatg atcgcgtccc
    10741 cgggcagcgg ccggcagcac ttgccgaagc tgaccaccgt gccctcggtg cccttgatca
    10801 gcagcggcgc gccctggccg gcacgcgggc ggtcctcggc cgactcgccg ccgggcagca
    10861 ggcgcttggc gatcagcggc gccatgcgcg tgccgaggcc gatgtcggcg aggatctcgt
    10921 cgaagcccgc gacgccgagt tccttcgtca gcgcctgcac gcgcgcctgc ggcaggtcgg
    10981 caaggctgcc gacctggccg accaggctct tttcgagcag gcgcttgccg agcagcacgg
    11041 cctcgtcgcg ctgcaggttc ttcaggtagt gacggatgct cgcgcgtgcc ttggcggtga
    11101 cgacgaaacc gagccaggcc ggattcgggc gcgcggtcgg cgtgatgatg acctcgacgg
    11161 tctggccgtt ggcgagcggc gtgcgcagcg gcaccagccg gcggtcgacc ttggcggcga
    11221 cgcaggtgtt gccgacatcg gagtggatcg cgtaggcgaa gtcgaccgcg gtcgcgccct
    11281 tgggcagctc gatgatctcg ccgcgcggcg tgaacacgta gacctcgtcc gggaagagat
    11341 ccatcttcac gctttcgagg aactcgaccg aattgccggc ccggcgctgc atgtcgagca
    11401 gccgtgccag ccattcgcgc gccctggcgt gcgcggcgtt gccctgcgcg cccggcgact
    11461 tgtacagcca gtgcgcggca acaccggcct cggccatcac gtgcatgtcc tgcgtgcgga
    11521 tctgcacctc gatcggcacg ccgtgcggtc cgaacagcac ggtgtgcagc gactggtagc
    11581 cgttggcctt cgggatcgcg acgtagtcct tgaagcggcc catgatcggc ttgtacaggc
    11641 cgtgcacgag gccgagcgtg cggtagcagg tatcgacgct gtcgacgatc aggcgcacgg
    11701 cgtagacgtc gtagacctcc ttcaggccga ggcgcttctc gtgcatcttc ttgtagatgc
    11761 cccagagatg cttctcgcgc ccgaccacgc gcgccttgat gccctcttcg agcaggcggt
    11821 tgcgcacgcg cgtctcgatc tgggaaatga tctcgcggcg gttgccgcgc agcttgcgca
    11881 ccgcctcgga cagcacgcgg tggcgcgtcg gatacaggta cgcgaagccg aggttctcga
    11941 gttccagccg caggtggttc atgccgaggc ggccggcgat cggggcgtag atctccaggg
    12001 tctcgcgggc gatgcgccgg cgcttctcgg ccggcatcgc gccgagcgtg cgcatgttgt
    12061 gcaggcggtc gccgagcttg accatgatca cgcggatgtc cttcgccatc gcgaggaaca
    12121 tcttgcggaa gttctcggcc tgcgcctcca gcttcgagcc gaactggatc tgcgtgagct
    12181 tggtgacgcc gtcgaccagt tcggcgacct cggggccgaa gcgctcggtg acctggtcct
    12241 tgccgaactt ggtgtcctcg atgacgtcgt gcagcacggc cgccatcagg gtctcgtggt
    12301 cgaaatgcac ctcggcgagg atgcgcgcga ccgcgatcgg gtggaacacg tagggctcgc
    12361 cgctcttgcg gtactgcccc tggtgcgctt cggccgagaa ccagcaggcc gagcgcacgg
    12421 cctcgacctg tgcggggtcg aggtagccgg ccagcaccat gcacagctcc tcgatccggg
    12481 agttcagctc ctcggaccag tcgctgccgg gcagcacggc gctcgacgcg ctcaccgatg
    12541 cctcaacgcg atcgctcgtg ccgcagcggg cggagcttca ggccgcacgc gctcacgcgt
    12601 tcgcctcgcc gtcgaggccg ggggccggcg gcgtgaccgg cgaaggttcg ccgccggcca
    12661 tgtccagcgg ctcatcgaag ctgaaatccg ccagcaccgg ctcgcgctcg cgctccggca
    12721 gcacctgcgg ctcgcgcatc agcgcctgcg gatcgatcag gccggcggcc agttcgcgca
    12781 gcgcgatgac ggtcggcttg tcgttttcct cgggcaccag cggcatctgg ccgagtgcga
    12841 tctggcgcgc gcggcgcgag gcgaccagca cgagctggaa gcggttctcg acgcgatcca
    12901 gacaatcttc aacggtgacg cgtgccatgt tcgggatttc ctcgaagatt caacgggtga
    12961 cgacgctgcc ggccgaccgt acggcccgca cgacgcgcac cccggacaaa agtgagtcag
    13021 tgtacgcaga cgcccccggc tgccacaacc gccgtcccgg cgtgccggcc ttcagtcgag
    13081 cagcgcatcg agcagcgcgc cgtgacgctc ggcctgcacg ctgcggcgca ggcgccgggc
    13141 ggtgaacagc gcctgcagct cggccagcgc ccggccgaag tcgtcattga cgatcaggta
    13201 atcgaattcg acccagtgca tgagttcgga gcgcgcatcg cgcatgcgcc gctcgatgac
    13261 ctcggccgcg tcctggccgc ggccgctcag gcgccggcgc agttccccga ccgagggcgg
    13321 cacgatgaac accgactgcg cttccggaaa cgcggcccgc acctgacgcg cgccctgcca
    13381 gtcgatctcg aggatcacgt cgcgcccggc cgcgagccgc gccgcgacgc cggtgcgggc
    13441 ggtgccgtag cggcggtcga acacctgcgc gtgttcgagg aaggcatcgt cggcgaccag
    13501 ccgtgcgaag gcatcgtcgc tgacgaagtg gtagtgctcg ccgtcgcgct cgccggggcg
    13561 cggagcgcgc gtcgtgtgcg acaccgagac ctcgacctgc acatcctgcg tcagcagcgc
    13621 gcgcacgagg ctggtcttgc cggcgcccga gggcgccgaa acgatgtaga gctggccgga
    13681 tgcggtcatc gcggggactc cgcgcggggc ttgccgccct gcgctcattc gatcattcga
    13741 tgttctggat ctgctcgcgc atctgctcga tcagcacctt cagctccacg gcggcgcgcg
    13801 tggtctcggt atcgagcgat ttcgacgaca gcgtgttggc ctcgcggttg agctcctgca
    13861 tcaggaagtc gagccgccgg ccgaccggct cgggccggcg cagcacgtcg cggatctcgg
    13921 cgaggtgggc atcgagccgg tcgagttcct cctcgacatc catgcgctgc gcgacgatga
    13981 ccatctcctg ttcgagccgg cccggatcgg cgccggccgg caggtcggcg aggcgcccgg
    14041 tgagcttctc gcgcagccgc gcgaccacct cgggccggcg ctggcgcacg aacgcgacct
    14101 gggcggcgat cgcatcgcag cggctgagga tcatctcggc ggtgcgcgcg ccctcgcgct
    14161 cgcgggtcgc gaccagctcg gcgagcgcgg cgtcgagcac ctccagcacg ccacgcttca
    14221 gcggttcgag atcctgctcg gcctcggtga ccacgcccgg ccagcgcagc acatcggcga
    14281 gacgaagccc ggtgccggga ccgatcaggt tttcgagctc gccggcaatg ccgagcaggc
    14341 gctcggcgta ggcgcggttc agcgtcagct ccggcgcgct gctggtggcc ggctggaagc
    14401 gcaggctggc gtcgaccttg ccgcgcgtca gccggccctg cacgcgctcg cgcacggcca
    14461 gatcgagcgc acgcagggtc tcgggcatgc gcagcgagat ctccagatag cggtgattca
    14521 ccgtgcgcag ctcccaggcg atggcaccgg gttcggtgtg gcgttcgcac cgggcgaagg
    14581 cggtcatgct gcggatcatc agggaacagt cctcgggaag cggcgggcgg gcggcgcagg
    14641 gggcgcggcg acggtggccg tgcatggtag ccccgggcag tcggggcgca aaccgcggcg
    14701 gctcgccgtg cccggggctt cggatatact tcgcgccctt ttgcctgccc gtcccccaca
    14761 ggtgcccccc atgcgtccca gcggccgcgc gcccgagcag ctccgcccga tccgtctcac
    14821 ccgccagttc acccgccacg ccgaaggctc ggtgctcgtc gagttcggcg agacgcgcgt
    14881 gatctgcacg gccagcgtcg aggaacgcgt gccgccgttc ctgaagggca agggccaggg
    14941 ctggatcacg gccgagtacg gcatgctgcc gcgcgccacg cacacccgct cgccgcgcga
    15001 ggcggcgcgc ggcgcgcaga gcgggcgcac gcaggagatc cagcgcctga tcggccgcag
    15061 cctgcgcgcc tgcgccgatc tcgccgcgct cggcgagcgc acgatcacga tcgactgcga
    15121 cgtgatccag gccgacggcg gcacgcgcac ggccgcgatc accggcggct tcgtcgcgct
    15181 cgccgatgcc gtgcgcacgc tgctcgaacg ccggtcgctg aagaagaacc cgctgcacgg
    15241 ccagatcgcc agcgtctcgg tcggcatctg gcgcggcacg ccggtgctcg acctcgacta
    15301 cgccgaggat tccgaggccg aaaccgacat gaacgtggtc atgaacgatg ccggcgccta
    15361 catcgaggtg cagggcacgg ccgaaggtca tgcgttccgg gccgaggaac tcgacgcgat
    15421 gctcgcgctc gcgcgcgccg gcatcgccga gctgatcggc caccagcgcg ccgcgctcgg
    15481 catcggccgc tgaggcctca gccttcggcg cgctccgcgg tgcggcgcag gcggccgagc
    15541 gtgacgcgca cgcgatccag ttcggtacgc gcgaccagcc gcggcagcgc cgattcgagt
    15601 tcggagagcg ccgcgtgcag atcctcgggc gtcgtgaccg cttcgagccg ctcgatccgc
    15661 cgcgccgccg cagcgttcat gcgtccgccg accggaccga cggattcggc gagcagcgcc
    15721 agcgtgcgcc ggcgcacctg atcgagcgcg gccggcaggc ccgtgaccgg cacgctggcg
    15781 gcctcgacct gcacggccac cagcccggcc agggccgtgc gcacacgttc gagatcgcgc
    15841 cgcccgacca gccggggcag cgcggcatcg agttcgccga gggcggcacg ccagccgtcg
    15901 gcatcgcggg cgccgttcag acgctccatc tgccgcgccg cgccggcgcc gagccggcca
    15961 ccgaccggcc cg
//
LOCUS       DAOQIE010000046        15338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1853_length_15338_cov_7.883531, whole genome shotgun sequence.
ACCESSION   DAOQIE010000046 DAOQIE010000000
VERSION     DAOQIE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_15338_cov_7.883531"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /gene="lgt"
                     /locus_tag="PK144_04640"
     CDS             complement(<1..772)
                     /gene="lgt"
                     /locus_tag="PK144_04640"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNF65049.1"
                     /translation="MDVLIWSVDPEIVRFGGFTLRWYGVLFATAFLAGYEIMRRIYHA
                     EGKPEDELDTLLIYMMLGTVLGARLGHVLFYDPAYYLAHPLRILAVWEGGLASHGGAI
                     GIFLALWLYTRRRPDQPYLWLLDRLSIPTAFGGACIRTGNLFNSEILGNPSELPWAVV
                     FARVDVIPRHPVQVYEALAYLVIFLGLYALYRRTRRATPEGRLLGSFLVAVFGVRFLL
                     EFLKTPQAAYESGFAITVGQWLSLPLIAVGVWLLLRSRR"
     gene            complement(778..1857)
                     /locus_tag="PK144_04645"
     CDS             complement(778..1857)
                     /locus_tag="PK144_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF65050.1"
                     /translation="MNFVLAWFRRVFSDAQAVSLIIVIVTLVAIVLLLGRMLAPVLAG
                     IVIAYLLEGLVAKLERAGLQRWPAVLIVFFGFMGLVVFALTGPLPLLSEQATQLARQL
                     PDMLLKAQKYLLSLPEQYPRVVSEQQIADAIAMVKREIAGVGQNLLTWSLASVLSVIT
                     VIVYLILVPVLVFFFLKDKVPIVRWCRQFLPADTRLAGRVWDDVDRQIGNYVRGKVWE
                     VLVNWVICYLVFAWLGLQYAMLLGFMVGLSVIVPYIGAAVVTVPVALVGLFQWGWGPD
                     FMWLIGAYAILMVLDGNVLVPWLFSEVVDLHPVAIIVAVLTFGGLWGFWGVFFAIPLA
                     TVVQAVLKIWPKAVVVAVPGHGGVS"
     gene            2072..2845
                     /locus_tag="PK144_04650"
     CDS             2072..2845
                     /locus_tag="PK144_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNF65051.1"
                     /translation="MNRPPETPLPAHAHAELLQRLQALIAYVSRRRQVEGDLTQIAAD
                     TASMFGAEACSIMLFKDGGSELRLHLTARSRPLPKELRGQGLSEAVGEGLAGRVAASG
                     RALLVADLATSEFAGISRHHTGSCICAPIPVAGRVRGVLNLSRPQPFTHDDLALACIA
                     GLLLGLALELARIQGLLRSRFAHRAMAEELREHPEMDAGSLGADAGRMARVLGRTFFR
                     ELRSAGYGSDHILTAATEVIGELGRDIGNSRKAGTVADD"
     gene            complement(2842..3816)
                     /locus_tag="PK144_04655"
     CDS             complement(2842..3816)
                     /locus_tag="PK144_04655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNF65052.1"
                     /translation="MPFAGLDPDCILDAVEAAGHRCDGRLLALNSYENRVWQVGREDD
                     TPLVAKFYRAGRWRDAQILEEHAWSTDLAAAELPVVAPLAAADGTTLFRHRGFRYALY
                     PRHGGRWPPLDDPETLYRLGRLLGRIHAHGAQLPFRERPALDVERFGHEPRATVLAGS
                     WLPAYLREAYATLSRDLLAAIEAAFAGLHWRSIRLHGDCHPGNLLWTDEGCHFVDLDD
                     AVSGPAVQDLWMLLSGERDERQLQLAELIEGYREFHDFDARELALIEPLRALRQLHYA
                     AWIARRWDDPAFPAAFPWFAGPRYWEEHVLALRECLAALGEPPLSLWD"
     gene            complement(3822..4406)
                     /locus_tag="PK144_04660"
     CDS             complement(3822..4406)
                     /locus_tag="PK144_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HNF65053.1"
                     /translation="MRLSAVLRRSLLCAVLAVAGGCALSPQTLNIHPSLDVAAPPLGR
                     GRPLMLEVIDARTQPAVFGTRGGVYQTATVGPRNDVAAAVRQALAERLTAAGFVLSAP
                     QPGSRTLRVELLRIDYHASGEPAVGEVRTSCEVRAIAGNAASSYSGNYRANSVRQVVG
                     SPGVEDNEQIVNEIISQALQRMLADRGLLETLAR"
     gene            4563..6032
                     /locus_tag="PK144_04665"
     CDS             4563..6032
                     /locus_tag="PK144_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNF65054.1"
                     /translation="MADDCAIALGQSHLNLYQRIAEDLATAIANGEHVPGERVPSVRE
                     LARRLGVSISTVTQAYGLLVDRGVLEARPQSGYYVGLRARASAPEPSKSRPAVQPMPV
                     STSALTLEVLAGAGDPGLINLGNAVAAPDLLPTASINRCLMSVIREMPELSAVYDAPF
                     GRPELRAQVVRLAHGAGVRAAPEEVLITGGCQEGVTLCLRALARPGDTIAVESPTFFG
                     TLQAIESLGMHALEIPTHPRTGICLDALEQALAQWPIRACVVMPTNANPVGSCMELAE
                     RQKLVELLARHEIPLIEDDACGDLGFAPGPRPPACKAWDRDGGVLLCSSFSKTLGPGY
                     RLGWIAAGRFAERVRHLKQMSSFAPAPLQQVAIARFLARGSYDRHLARARAGYAQRSA
                     RVAAAVAQHFPAGTRVAQPAGGTVLWVELPEGLDTLTLYRRAHEARISFAPGALFTAQ
                     KRYRNALRLYTAPRFDERMDTALRTLGLLAQQMQDEAVA"
     gene            complement(6137..6376)
                     /locus_tag="PK144_04670"
     CDS             complement(6137..6376)
                     /locus_tag="PK144_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNF65055.1"
                     /translation="MYVCICKGITDRDIRNAAARGVSTVSALKRETGCCGQCGKCAWH
                     AREVLDAVRECVQPAAVVAATLGFGAATEAMPAMA"
     gene            complement(6467..6946)
                     /gene="bfr"
                     /locus_tag="PK144_04675"
     CDS             complement(6467..6946)
                     /gene="bfr"
                     /locus_tag="PK144_04675"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNF65056.1"
                     /translation="MKGDAKVIEFLNKVLKNELTAINQYFLHAKMFKNWGFHKLAGHE
                     YEESIDEMKHADKLIERILFLEGLPNLQDLGKLCIGENVVEALKADLDMELKAHPVLK
                     EAIAHCETIQDYVSRELFEDILESEEEHIDWLETQLGLVGMVGEPNYLQSAMGEGGS"
     gene            complement(7030..7467)
                     /locus_tag="PK144_04680"
     CDS             complement(7030..7467)
                     /locus_tag="PK144_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF65057.1"
                     /translation="MNVPDADKDRQLLALLSANAREPLASLARKLGVARSSLQERIGR
                     LERSGVIRGYGVRLGAALAPATRAFFLVQLDGPLCERIAPQLAGLAEVVACHSIAGDI
                     DMLLEVAAGDLAGLDAVRARIGALAGVRRVQTLPVLKTRFERR"
     gene            7609..7803
                     /locus_tag="PK144_04685"
     CDS             7609..7803
                     /locus_tag="PK144_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNF65058.1"
                     /translation="MSLEMKPACERCRQPLPPDSPDARICSYECTYCRDCSEHRLHGR
                     CPNCGGELLARPRRLPTAGG"
     gene            complement(7787..8941)
                     /locus_tag="PK144_04690"
     CDS             complement(7787..8941)
                     /locus_tag="PK144_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF65059.1"
                     /translation="MTAPLVSRLPRVGTTIFTVMSALAQECGALNLSQGFPDFAVPPA
                     LLQAVVQHLQAGHNQYAPMTGVPRLREAIAAKTLAAYGVAPDPVHEVTVTSGATEALF
                     CAIQAVLRAGDEAIVFDPCYDSYEPGIELAGGRAVHVPLAGPDFRPDWQRFAGALSPR
                     TRLVILNTPHNPTGSVWSAEDMQQLATLLRGRGIWLLSDEVYEHIVFDGRRHESVLLH
                     PELAARAFVVSSFGKTFHATGWKIGYCIAPAALSAEFRKIHQFVTFSTSTPMQHALAD
                     YLEAEAQHWQQLPAFYQRKRDLFCRLIEGSRFGCTPSAGTYFQLLDYSAISDERDVDL
                     ARRLTREARLASIPVSVFSVAPDPAQRLLRFCFAKDDATLEQAAAIIRRL"
     gene            9548..11470
                     /locus_tag="PK144_04695"
     CDS             9548..11470
                     /locus_tag="PK144_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNF65060.1"
                     /translation="MSIFERYATRYEESREEVLSLQEYLELCRSDPSAYASPAERLLM
                     AIGEPELIDTRLDPRLSRIFQNKVIKVYPAFREFYGMEDSIDQIVSYLRHSAQGLEER
                     KQILYLLGPVGGGKSSLAEKLKHLMEKVPFYAIQGSPVNESPLNLFDPDEDGRILEDD
                     FGIPRRYLKGLMSPWAVKRLKEFNGDITRFKVVRLHPSILKQVAVAKTEPGDENNQDI
                     SSLVGKVDIRKLEHYAQNDPDAYAFSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNYNGTEGLSAIPFDGIILAHSNESEWMAFRNNRNNEAFLDRVYIVKVPYCLRVSDE
                     VHIYEKLLISSSLSEAPCAPGTLQMLAQFSVLSRLKEPENSSIFSKMRVYDGENLKDT
                     DPKAKSIQEYRDYAGVDEGMAGISTRFAFKILSQVFNFDHTEVAANPVHLLYVLERQI
                     EREQFPAEVEEKYLSYLKGWLAPRYAEFIGKELQQAYLESYSEYGQNIFDRYVTYADF
                     WIQDQEYRDPDTGESFDRASLNSELEKIEKPAGISNPKDFRNEIVNFVLRARAANNGR
                     NPVWTSYEKLRAVIEKKMFSNTEDLLPVISFNAKSSADEQKKHEDFVNRMTQKGYTAK
                     QVRLLSEWYLRVRKSS"
     gene            11505..12773
                     /locus_tag="PK144_04700"
     CDS             11505..12773
                     /locus_tag="PK144_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNF65061.1"
                     /translation="MSHLVDRRLNGKNRSAVNRQRFLRRFKDQLKRAVADAMAGRSIK
                     DVDSGEKVSIPTRDLSEPVFSHGSGGRRETIHPGNKEFSTGDRVKRPQGGGGAGGGGK
                     QASKDGEGEDDFVFELSRDEFLDLFFEDLALPELIKTQLAKIPEWKNIRAGYTTAGVP
                     SNINVVRSLKGALARRTAIGAPHRTRLRELEQELEEILASEGEGSPEAERLREEIRHL
                     RFRIEAIPFIDTFDLRYNNRIRQPKPTTQAVMFCIMDVSGSMDQERKDLAKRFFTLLY
                     LFLTRNYERIEVVFIRHHTVAKEVDEQEFFYSRETGGTVVSSALSLMADIVSERYSPG
                     AWNIYAAQASDGDNWQDDSPQCREILSARIMPFVQYFAYVEITPDRHQSLWEEYQKLQ
                     TTHKQFAMQEIGGAEDIYPVFRELFKRRMA"
     gene            12770..14275
                     /locus_tag="PK144_04705"
     CDS             12770..14275
                     /locus_tag="PK144_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNF65062.1"
                     /translation="MSGKRLISEGSEWTFPLLETYDREIARIASEQFRLDTFRNQIEV
                     ITAEQMMDAYSSVGMPIGYHHWSFGKQFVATEKHYRRGQMGLAYEIVINSDPCIAYLM
                     EENTMTMQALVIAHACYGHNSFFKGNYLFRAWTSADSIIDYLVFARNYITRCEERYGI
                     ETVEELLDSCHALMNHGVDRYKRPAPLSMAKEQQRQREREEYLQLQVNDLWRTVPVRA
                     GAAVVEESARFPEEPQENLLYFIEKHAPLLEPWQREIVRIVRKIGQYFYPQRQTQVMN
                     EGWATFWHYTLLNRLYEEGRVSDGFMIEFLQSHTNVVFQPEFDSPYYSGINPYALGFA
                     MMSDLRRICENPTPEDRRWFPEIAGGDWLKVLDFAMRNFKDESFIAQYLSPKLIRDFK
                     LFAVLDDDKADTLEITAIHDDDGYRKVRQSLSDQYNLGSREPNIQVWNVDRRGDRSLT
                     LRHSVYNRRPLNAGTTYEVLKHMFRLWGFTVRLEAVDENGKVSLVNEVRKH"
     gene            14399..15241
                     /gene="mepA"
                     /locus_tag="PK144_04710"
     CDS             14399..15241
                     /gene="mepA"
                     /locus_tag="PK144_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HNF65063.1"
                     /translation="MLRPLLSSLLLVPALAAAGPWGEVRLPSAELPQVYGGYAAGCIA
                     GAQTLPLSGPGYQAMRPSRNRYYGHPELLRFIEQLGYTAAARRFAPVLIGDLGQPRGG
                     PMLSGHRSHQIGLDVDVWFLDAPGGRAPSGAALESLSAQTMIDPYAGRVLARFGARQR
                     ALLRAAADDPAVERIFVNPVIKQSLCRSEADRGWLHKIRPWWGHDAHFHVRLYCPPDS
                     PQCSAQKPIEPGDGCDAGLDAWVEEIRQAARRPPLPTPPAPEPVLPEACTAVLQAPSA
                     SARR"
BASE COUNT         2698 a         5334 c         5023 g         2283 t
ORIGIN      
        1 cccgccgcga acgcagcagc agccagacgc cgaccgcgat cagcggcagg ctcagccact
       61 gtccgaccgt gatcgcgaag ccgctctcgt aggcggcctg cggcgtcttc aggaattcga
      121 gcaggaagcg cacgccgaac accgcgacca ggaagctgcc gagcagccgg ccctcgggcg
      181 tggcgcggcg cgtgcgccgg tacagcgcat acagcccgag gaagatcacg agatacgcga
      241 gcgcctcgta gacctgcacc gggtggcgcg ggatcacgtc gacgcgcgcg aacaccacgg
      301 cccacggcag ctcgctcggg ttgccgagga tctcggagtt gaacaggttg ccggtgcgga
      361 tgcaggcacc gccgaacgcg gtcgggatgc tcaggcgatc gagcagccac agatacggct
      421 ggtcgggacg ccggcgcgtg tacagccaga gcgccaggaa gatgccgatc gcgccgccgt
      481 ggctcgcgag cccgccctcc cagaccgcga ggatgcgcag cggatgcgcg aggtaatagg
      541 ccgggtcgta gaacagcacg tgcccgagcc gggcgccgag cacggtgccg agcatcatgt
      601 agatcagcag cgtgtcgagt tcgtcctcgg gcttgccctc ggcgtgatag atgcggcgca
      661 tgatctcgta accggccagg aaggccgtcg cgaacagcac gccataccag cgcagcgtga
      721 agccaccgaa ccggacaatc tcggggtcga cgctccagat cagcacgtcc atcggaatca
      781 gctcactccg ccgtgcccgg gcacggccac gacaaccgcc ttcggccaga tcttcagcac
      841 cgcctgcacg acggtcgcca gcgggatcgc gaagaacacg ccccagaacc cccacaggcc
      901 gccgaaggtc agcaccgcga cgatgatcgc caccgggtgc aggtcgacga cctcgctgaa
      961 cagccacggc accagcacgt tgccgtcgag caccatcagg atcgcgtagg ccccgatcag
     1021 ccacatgaag tccggccccc agccccactg gaacagcccg accagcgcca ccggcacggt
     1081 cacgaccgcc gcgccgatgt agggcacgat caccgacagc ccgaccatga agccgagcag
     1141 catcgcgtac tgcaggccga gccaggcgaa caccaggtag cagatcaccc agttgaccag
     1201 cacctcccag accttgccgc gcacgtagtt gccgatctgg cggtcgacat cgtcccagac
     1261 ccggccggcg aggcgggtgt cggccggcag gaactgccgg caccagcgca cgatcggcac
     1321 cttgtccttc aggaagaaga acaccagcac cggcaccagg atcaggtaga cgatgacggt
     1381 gatgacgctg agcaccgagg ccagcgacca ggtcagcagg ttctggccga cgccggcgat
     1441 ctcgcgcttg accatcgcga tcgcatcggc gatctgctgc tcgctgacca cgcgcgggta
     1501 ctgctcgggc aggctcagca ggtacttctg cgccttcagc agcatgtccg gcagctggcg
     1561 cgcgagctgc gtggcctgct ccgagagcag cggcagcggc ccggtcagcg cgaacaccac
     1621 gagccccatg aagccgaaga acacgatcag caccgccggc cagcgctgca gcccggcgcg
     1681 ttcgagcttg gcgaccaggc cttcgagcag gtacgcgatg acgatgccgg cgagcaccgg
     1741 tgccagcatc cgcccgagca acagcacgat cgcgaccagc gtgacgatga cgatgatcag
     1801 cgacacggcc tgagcatcgc tgaacacgcg cctgaaccag gcgaggacga aattcatgca
     1861 ggctccggtc gaccggtcct tcgccggcgg atgatttgaa gaatctgaaa aaggacgggg
     1921 gcaggacaga aaaccgggcg gatcgcgcag ccggggtgga cgatgcaggg gccgctacgg
     1981 ctgcagccgc atcggcgcga tgcgggccga cactctacca tccaacgcca tcgagccgct
     2041 ttcacgacga tccccacgga ggtccgtgcc gatgaaccgc ccccccgaaa cgccgctgcc
     2101 cgcccatgcc cacgccgagc tgctgcagcg cctgcaggcc ctgatcgcct acgtcagccg
     2161 ccgccggcag gtcgagggtg acctgacgca gatcgccgcc gacaccgcct cgatgttcgg
     2221 cgccgaggcc tgctcgatca tgctgttcaa ggacggcggc agcgagctgc gcctgcacct
     2281 gaccgcgcgc tcgcgaccgc tgccgaagga actgcgcggc caggggctca gcgaggcggt
     2341 cggcgagggg ctggccggcc gggtcgctgc cagcggccgg gcgctgctgg tggcggatct
     2401 cgccacgtcc gaattcgccg gcatcagccg ccaccacacc ggcagctgca tctgtgcccc
     2461 gatcccggtc gccggacgcg tgcgcggcgt gctgaacctg agccgcccgc agccgttcac
     2521 gcacgacgac ctggcgctcg cctgcatcgc cggcctgctg ctcggcctgg cgctggagct
     2581 cgcccgcatc cagggcctgc tgcgctcgcg cttcgcgcac cgggcgatgg ccgaggaact
     2641 gcgcgagcat ccggagatgg acgccggcag cctcggcgcc gacgccggac gcatggcgcg
     2701 cgtgctcggg cggaccttct tccgcgagct gcgctcggcc ggctacggtt cggatcacat
     2761 cctgaccgcg gcgaccgagg tgatcggcga actcggtcgc gacatcggca acagccggaa
     2821 agccggaacg gtcgccgacg actagtccca cagcgacaag ggcggctcgc cgagcgcggc
     2881 gagacactcg cgcagcgcca gcacgtgctc ctcccagtag cgcgggccgg cgaaccacgg
     2941 aaaggccgcc gggaaggccg gatcgtccca gcgccgggcg atccaggcgg cgtagtgcag
     3001 ctggcgcagc gcgcgcagcg gctcgatcag cgccagctcg cgcgcatcga agtcgtggaa
     3061 ctcgcgatag ccctcgatca gttcggcgag ctgcagctga cgctcgtcgc gctcgccgga
     3121 cagcagcatc cagagatcct gcacggccgg gccgctgacg gcgtcgtcga gatcgacgaa
     3181 gtggcagcct tcgtcggtcc agagcaggtt gccggggtgg cagtcgccgt gcagccggat
     3241 cgaccgccag tgcaagcccg cgaaagcggc ctcgatcgcg gcgagcaggt cgcggctgag
     3301 cgtggcatag gcctcgcgca gatacgccgg cagccaggac ccggcgagca ccgtcgcgcg
     3361 cggctcgtga ccgaagcgtt ccacgtcgag cgccggccgc tcgcgaaacg gcagttgcgc
     3421 gccatgcgcg tggatgcgcc cgagcaggcg gccgagccgg tacagcgtct ccgggtcatc
     3481 gagcggcggc cagcggccgc cgtggcgcgg atacagcgca taacggaagc cccggtgacg
     3541 gaacagcgtc gtgccatcgg ccgccgcgag cggcgcgacc accggcagct cggccgcggc
     3601 aaggtcggtg ctccaggcgt gttcttcgag gatctgcgca tcgcgccagc gcccggcccg
     3661 gtagaacttc gcgaccagcg gcgtgtcgtc ctcccgaccc acctgccaga cccggttctc
     3721 gtagctgttc agcgccagca gccggccgtc gcagcgatgg ccggcggctt cgacggcgtc
     3781 gaggatgcag tccggatcga gaccggcaaa cggcagacgg ttcaccgggc gagtgtttcg
     3841 agcaggccgc ggtcggcgag catgcgctgc agggcctggc tgatgatctc gttgacgatc
     3901 tgctcgttgt cctcgactcc gggcgagccg accacctggc gcacgctgtt cgcgcggtag
     3961 ttgccgctgt agctgctggc ggcattgccg gcgatggcgc gcacctcgca actggtgcgg
     4021 acctcgccga ccgccggctc gccgctggcg tgatagtcga tgcgcagcag ctcgacgcgc
     4081 agcgtgcggc tgccgggctg cggcgccgac agcacgaagc ccgctgccgt cagccgttcg
     4141 gcgagcgcct ggcgcacggc cgccgccacg tcgttgcgcg gcccgaccgt ggcggtctga
     4201 taaaccccgc cgcgggtgcc gaacaccgcc ggctgtgtgc gcgcatcgat gacttcgagc
     4261 atgagcggcc ggccgcgacc gagcggcggc gcggccacgt cgagcgaggg atggatgttc
     4321 agggtctgcg gactcagtgc gcagccgccc gccacggcga ggacggcaca cagcaggcta
     4381 cgacgcagaa cggcggacag gcgcatgcga ccgactccgg agcgtgttga ccgcgtgcag
     4441 cataccgcag agcaccaccg ctgccggacg ccgtgttcac cgctgctgac acggccgggg
     4501 ctgcgccgca cagcacagtc tgcaagaata tcgtccatca ccagcacaga cgatgcttga
     4561 tcatggccga cgactgcgcc atcgccctcg ggcaatccca tctgaacctg taccagcgca
     4621 tcgccgagga tctggccacc gcgatcgcca atggcgagca cgtccccggc gagcgcgtgc
     4681 cctcggtgcg cgagctggcg cgccggctcg gcgtcagcat ctcgacggtc acccaggcct
     4741 acggcctgct ggtcgatcgc ggcgtgctcg aagcccggcc gcagtccggc tactacgtcg
     4801 gcctgcgcgc acgcgccagc gcaccggaac cgtccaagtc ccgcccggcc gtgcagccga
     4861 tgccggtgtc caccagtgcg ctgacgctcg aagtgctggc cggggccggc gatccggggc
     4921 tcatcaacct cggcaacgcg gtcgccgcgc ccgacctgct gccgactgcg tcgatcaacc
     4981 gctgcctgat gtcggtgatc cgcgagatgc cggagctgtc ggcggtctac gacgcgccct
     5041 tcggccgccc ggagctgcgt gcgcaggtcg tgcgcctcgc gcatggcgcc ggcgtgcgcg
     5101 cagcccccga ggaagtcctg atcaccggcg gctgtcagga aggcgtgacg ctctgcctgc
     5161 gcgcgctcgc ccgccccggc gacacgatcg cggtcgaatc gccgaccttc ttcggcacgc
     5221 tgcaggcgat cgaatcgctc ggcatgcacg ccctggagat cccgacccat ccgcgcaccg
     5281 gcatctgtct cgacgccctc gaacaggcgc tcgcgcagtg gccgatccgc gcctgcgtcg
     5341 tgatgccgac caacgccaat ccggtcggct cgtgcatgga gctggcggaa cgccagaagc
     5401 tcgtcgaact gctcgcgcgt cacgagatac cgctgatcga ggacgatgcc tgcggcgatc
     5461 tcggcttcgc acccggcccg cggccaccgg cctgcaaggc ctgggaccgc gacggcggcg
     5521 tgctgctgtg ctcgtcgttc agcaagacgc tcggccccgg ctaccggctc ggctggatcg
     5581 ccgccggccg tttcgccgaa cgcgtgcggc atctgaagca gatgtcgagc ttcgccccgg
     5641 cgccgctgca gcaggtggcg atcgcacgct tcctcgcccg cggcagctac gatcgccacc
     5701 tcgcccgcgc ccgcgccggc tatgcgcagc gctcggcgcg cgtggcggcg gcggtcgccc
     5761 agcattttcc ggccggcacc cgcgtcgcgc agccggccgg cggcaccgtg ctctgggtcg
     5821 aactgccgga ggggctcgac acgctgacgc tgtaccggcg cgcgcacgag gcacgcatca
     5881 gctttgcccc cggggcgctg ttcacggcac agaagcgcta ccgcaatgcg ctgcgcctgt
     5941 acacggcgcc gcgcttcgat gagcgcatgg acacggcgct gcgcacgctc ggcctgctcg
     6001 cgcagcagat gcaggacgaa gccgtcgcct gaacccggtg ccctgcctgc gccaagcccc
     6061 gcagagcagc gcaaaaacgc acaaaacaga aagccccggt cacccggggc ttttcgttgc
     6121 tgctgcgccg gccggctcag gccattgccg gcatcgcctc ggtggcagcg ccgaaaccca
     6181 gcgtcgccgc gaccaccgcc gccggctgca cgcactcccg cacggcatcg agcacctcgc
     6241 gcgcatgcca cgcacacttg ccgcactgtc cgcagcaacc ggtctcgcgc ttgagcgccg
     6301 acaccgtgct gacgccgcgt gcggcagcat tgcggatgtc acgatcggtg attcccttgc
     6361 agatgcagac gtacatgaac ggcgctcccg gcaggcgtgg acggtatggc aggcatcatt
     6421 tcgcaaatga gaatgcctgt caacagcgag ccggaaaccg ctgcgatcag gagccgccct
     6481 cgcccatcgc cgactgcagg tagttcggct cgccgaccat gccgaccagt ccgagctgcg
     6541 tctccagcca gtcgatgtgc tcctcctcgc tttcgaggat gtcctcgaac agctcgcgcg
     6601 agacgtagtc ctggatcgtc tcgcagtggg cgatggcttc cttcagcacc gggtgcgcct
     6661 tcagctccat gtcgagatcg gccttcagcg cctcgacgac gttctcgccg atgcagagct
     6721 tgccgaggtc ctgcaggttc ggcaggcctt cgaggaacag gatgcgctcg atcagcttgt
     6781 cggcatgctt catctcgtcg atcgattcct cgtactcgtg cccggcgagc ttgtggaagc
     6841 cccagttctt gaacatcttc gcgtgcagga agtactgatt gatcgcggtc agctcgttct
     6901 tgagcacctt gttcagaaac tcgatgacct tggcgtcgcc tttcatggac ggttactcct
     6961 gctgggggtt tgcggtgggt gaaagcgtat agccgccggc gggcgtcctc gcgagcctga
     7021 gcgttcaggt caacgccgtt cgaagcgcgt cttcagcacc ggcagcgtct gcacgcggcg
     7081 cacgccggcc agtgccccga tgcgggcccg cacggcgtcg agaccggcca gatcgccggc
     7141 ggcgacttcg agcagcatgt cgatgtcgcc ggcgatcgag tgacaggcca cgacctccgc
     7201 caggccggcg agctgcgggg cgatgcgctc gcacagcggt ccgtcgagct gcacgaggaa
     7261 aaacgcccgc gtcgccggcg cgagcgccgc accgaggcgc accccgtagc cgcggatcac
     7321 gcccgagcgt tcgagccggc cgatgcgctc ctgcaggctg ctgcgtgcga cgccgagctt
     7381 gcgcgcgagg ctcgcgagcg gttcgcgggc attcgcactg agcagcgcca gcagctggcg
     7441 atccttgtcg gcatcgggga cgttcatcgg cggcgacctg ggcggcacgg ggggcttgcg
     7501 ccggccgcgg ccggcggatg tcgggggcgg ccggtcgatg cgccgggtcg aaatccggcc
     7561 gtcccggcag cgagcatgcc agcacctgcc ctgcccgagg aaccatcgat gtcactcgag
     7621 atgaaacccg cctgcgaacg ctgccggcag ccgctgccgc cggactcccc cgacgcccgc
     7681 atctgttcgt acgaatgcac gtactgccgc gactgcagcg agcaccggct gcatggccgc
     7741 tgcccgaact gcggcggcga gctgctggcg cggccgcgcc gcctgcctac agccggcgga
     7801 tgatcgccgc cgcctgttcg agcgtcgcgt cgtccttggc gaagcagaag cgcagcagcc
     7861 gctgcgccgg atcgggcgcg acgctgaaca ccgataccgg aatcgacgcg agccgcgcct
     7921 cgcgcgtcag ccgccgcgcc agatcgacat cccgctcgtc gctgatcgcc gaatagtcga
     7981 gcagctggaa gtaggtgccg gccgagggtg tgcagccgaa gcgcgaaccc tcgatcagcc
     8041 ggcagaacag atcgcgcttg cgctggtaga aggccggcag ctgctgccag tgctgcgcct
     8101 cggcctccag gtaatcggcc agcgcgtgct gcatcggcgt cgaggtcgag aaggtcacga
     8161 actggtggat cttgcggaac tccgccgaca gcgccgccgg tgcgatgcag tagccgatct
     8221 tccagccggt ggcatggaag gtcttgccga acgaggacac gacgaaggcc cgcgccgcca
     8281 gctccggatg aagcagcacg ctctcgtgcc ggcggccgtc gaagacgatg tgctcgtaga
     8341 cctcgtcgga cagcagccat atcccgcgcc cgcgcagcag cgtcgcgagc tgctgcatgt
     8401 cctcggccga ccagacgctg ccggtcgggt tgtgcggcgt gttgaggatg acgagccgcg
     8461 tgcgcggact cagcgccccg gcaaagcgct gccagtccgg ccggaaatcc ggccccgcca
     8521 gcggcacgtg cacggcccgg ccgccggcga gctcgatgcc gggctcgtag ctgtcgtagc
     8581 aggggtcgaa cacgatcgcc tcgtcgccgg cgcgcagcac cgcctggatc gcgcagaaca
     8641 gcgcctcggt cgcgcccgag gtcacggtga cctcgtgcac cggatcgggc gcaaccccgt
     8701 aggccgcgag cgtcttcgcg gcaatcgcct cgcgcaggcg cggcacgccg gtcatcggcg
     8761 cgtactggtt gtgaccggcc tgcaggtgct gcacgaccgc ctgcagcagg gccggcggca
     8821 cggcgaaatc cggaaagccc tgcgacaggt tcagggcccc gcactcctgt gcgagcgcac
     8881 tcatgaccgt gaagatcgtc gtgccgacgc gcggcagacg cgaaacgagc ggcgccgtca
     8941 tgacgcggtc tcctcgggat ttcagctcgg ggtcggaggg aagcatgcgg acccgccagt
     9001 gtacgggatt cgcgcctgta cgagtccggc attgtcgaca ggcatcgatg caccaggaag
     9061 gacggcggct gcagcgcctt gcgccactgc aacgcacatt cgggaccgct cgcgcaccat
     9121 tgcgtagcac tctgcaccat ggcatgaacc agattgatgc agtacgccat tgcatgacgg
     9181 ctcgattgcg gcgatgcagc agaacgcgcc gggcttgcta cagtcgttca cgactgcccc
     9241 ccgacgccgg gcgggagcgg cggcatacgc tcggcagcat gtcgtcgccg gcagcgacca
     9301 tgctgcggac ggatgacaaa acggctacaa gcgctccgaa ccgccacccg ccgtcacggt
     9361 cctccctgag gcaggcccgg gaccggaggc agcagatccg gcgccaggca gcgccagaac
     9421 cccacaccgt cgacggcgac ctgcaccggt cggcggtccc ccctgcagga atccccgaaa
     9481 ttggtccggc tattgctagt ttcgggacag atcatcgtgc attccggcgc cagaagaggg
     9541 ttaggccatg agcatcttcg aacgatacgc cacgcgatac gaggaaagcc gggaagaggt
     9601 gctgagcctc caggaatacc tggaactctg ccgcagcgac cccagtgcct acgcctcccc
     9661 tgccgaacgc ctgctgatgg cgatcggcga gccggaactg atcgatacgc gcctcgaccc
     9721 gcgcctgtcg cgcatcttcc agaacaaggt catcaaggtg tacccggcct tccgggagtt
     9781 ctacggcatg gaggattcga tcgaccagat cgtctcctat ctgcgccact ccgcgcaggg
     9841 tctggaagaa cgcaagcaga tcctctatct gctcggcccg gtcggtggcg gcaaatcctc
     9901 gctggccgag aaactcaagc acctgatgga aaaggtgccg ttctatgcca tccagggttc
     9961 gccggtcaac gaatccccgc tgaacctgtt cgatccggat gaggacggcc ggattctcga
    10021 ggacgatttc ggcattccac gccgttacct gaaaggcctg atgagcccgt gggccgtcaa
    10081 gcgcctgaag gaattcaacg gcgacatcac ccgcttcaag gtcgtgcgcc tgcatccgtc
    10141 gatcctgaaa caggtcgcgg tggccaagac cgaacccggt gacgagaaca accaggacat
    10201 ttcctcgctg gtcggcaagg tcgacatccg caagctcgaa cattacgcgc agaacgatcc
    10261 ggatgcgtat gcattctcgg gcggcctgtg cctcgcgaac cagggcctgc tggaattcgt
    10321 cgagatgttc aaggcaccga tcaaggtgct gcacccgctg ctgaccgcca cgcaggaagg
    10381 caactacaac ggtacggaag gcctgtcggc gattccgttc gacggcatca tcctcgcaca
    10441 ctcgaacgaa tccgaatgga tggcgtttcg caacaaccgc aacaacgagg ccttcctcga
    10501 tcgcgtctac atcgtcaagg tcccgtactg cctgcgcgtt tccgatgaag tgcacatcta
    10561 cgagaaactg ctgatcagtt cctcgctgtc cgaagccccg tgcgcgcccg gcacgctgca
    10621 gatgctggcg cagttctcgg tgctgtcacg cctgaaggaa ccggaaaact ccagcatctt
    10681 ctcgaagatg cgcgtctatg acggcgaaaa cctcaaggac accgacccga aggccaagtc
    10741 gatccaggaa taccgcgact acgccggcgt cgacgagggc atggccggca tctcgacgcg
    10801 cttcgcgttc aagatcctgt cgcaggtgtt caatttcgac cacaccgagg ttgccgccaa
    10861 tccggtgcat ctgctgtacg tgctggagcg ccagatcgaa cgcgagcagt tcccggccga
    10921 ggtcgaggag aaatacctgt cctacctgaa gggctggctg gcgccgcgct atgccgagtt
    10981 catcggcaag gaactgcagc aggcctatct ggaatcgtat tcggaatacg gccagaacat
    11041 cttcgaccgt tacgtgacct acgcggactt ctggattcag gatcaggaat accgcgatcc
    11101 ggataccggc gaatccttcg accgcgcctc gctgaacagc gaactcgaaa agatcgagaa
    11161 gccggccggc atctccaacc cgaaggactt ccgcaacgag atcgtcaact tcgtgctgcg
    11221 tgcccgtgcc gccaacaacg gccgcaaccc ggtctggacc agttatgaaa aactgcgcgc
    11281 cgtgatcgag aagaagatgt tctcgaacac cgaggacctg ctgccggtca tcagcttcaa
    11341 cgccaagtcg tccgccgacg agcagaagaa gcatgaggac ttcgtcaacc gcatgaccca
    11401 gaagggctac accgccaagc aggtgcgtct gctttccgag tggtacctgc gcgtgcgaaa
    11461 gtcgtcctga cgcctgtcgc ccctgccccg cgaggaatgc ttccatgtcc catctcgtcg
    11521 atcggcggct caacggcaag aatcgcagcg ccgtcaaccg gcagcgcttc ctgcgccgct
    11581 tcaaggatca gctcaagcgc gccgtggccg acgccatggc gggtcgctcg atcaaggacg
    11641 tggacagcgg cgagaaggtc agcatcccga cccgcgacct gtcggagccg gtgttcagcc
    11701 acggctccgg cggtcgccgg gagacgatcc accccggcaa caaggagttc tcgaccggcg
    11761 accgcgtcaa gcggccgcag ggcgggggcg gggccggcgg tggtggcaag caggccagca
    11821 aggacggcga gggcgaggat gacttcgtct tcgagctgtc gcgtgacgag ttcctcgacc
    11881 tgttcttcga ggatctcgcg ctgccggagc tgatcaagac ccagctcgcg aagattccgg
    11941 aatggaagaa catccgcgcc ggctacacga ccgccggcgt gccgagcaac atcaacgtcg
    12001 tgcgctcgct caagggtgca ctggcgcggc gcaccgcgat cggcgcgccg caccgcacgc
    12061 ggctgcgcga actcgaacag gaactcgaag aaatcctcgc cagcgagggc gaaggttcgc
    12121 cggaggccga acgcctgcgc gaggaaatcc gtcacctgcg cttccgcatc gaagccattc
    12181 cgttcatcga caccttcgac ctgcgttaca acaaccgcat ccgccagccg aaaccgacga
    12241 cgcaggcggt gatgttctgc atcatggacg tgtccggctc gatggatcag gaacgcaagg
    12301 atctggccaa gcgtttcttc acgctgctgt atctgttcct gacccgcaat tacgaacgca
    12361 tcgaggtggt gttcatccgc caccatacgg tcgccaagga agtcgacgag caggagttct
    12421 tctattcccg tgaaaccggc ggcaccgtgg tgtcctcggc gctgtcgctg atggcggaca
    12481 tcgtttcgga gcgttattcg ccgggcgcgt ggaacatcta cgcggcccag gcttcggacg
    12541 gcgacaactg gcaggatgac tcgccgcagt gccgggaaat cctcagcgcg cggatcatgc
    12601 cgttcgtgca gtattttgcc tacgtcgaga tcacacccga ccggcatcag agcctgtggg
    12661 aggagtacca gaagctccag accacccaca agcagttcgc gatgcaggaa atcggcggag
    12721 ccgaagacat ctatccggta ttccgggaac tgttcaagag gcgcatggca tgagtggcaa
    12781 acggctgatt tccgaaggct cggagtggac atttccactg ctcgaaacct atgaccgcga
    12841 aatcgcgcgc atcgccagcg agcagttccg gctcgacacc ttccgcaacc agatcgaggt
    12901 gatcaccgcc gagcagatga tggatgccta ttcctcggtc ggcatgccga tcggctacca
    12961 tcactggtcg ttcggcaagc agttcgtcgc caccgaaaag cattaccggc gcggtcagat
    13021 gggcctggcc tacgagatcg tcatcaactc cgatccgtgc attgcctacc tgatggaaga
    13081 gaacacgatg accatgcagg cgctggtcat cgcgcatgcc tgctacggtc acaactcctt
    13141 cttcaagggc aattacctgt tccgggcctg gaccagtgcc gattcgatca tcgattacct
    13201 ggtgttcgcg cgcaattaca tcacccgctg cgaggagcgc tacggcatcg agacggtcga
    13261 ggaactgctc gactcctgcc acgcgctgat gaaccacggc gtcgaccgct acaagcgtcc
    13321 ggcaccgctg tcgatggcca aggaacagca gcgccagcgc gagcgcgagg aatacctgca
    13381 gctgcaggtc aacgacctct ggcgcacggt gccggtgcgc gccggtgcgg cggtggtcga
    13441 ggaaagcgcg cgcttccccg aggaaccgca ggagaacctg ctgtatttca tcgagaaaca
    13501 cgcgccgctg ctcgaaccct ggcagcgcga gatcgtgcgc atcgtgcgca agatcggcca
    13561 gtatttctat ccgcagcgcc agacccaggt catgaacgag ggctgggcga ccttctggca
    13621 ttacacgctg ctgaaccggc tgtatgaaga aggccgggtc agtgacggct tcatgatcga
    13681 attcctgcag tcgcacacga atgtcgtgtt ccagccggaa ttcgattcgc cgtattacag
    13741 cggcatcaat ccgtacgcgc tcggcttcgc gatgatgagt gacctgcgcc gcatctgcga
    13801 aaacccgacg cccgaggacc ggcgctggtt cccggaaatc gccggcggcg actggctgaa
    13861 ggtgctcgac ttcgcgatgc gcaacttcaa ggacgagagc ttcatcgccc agtacctgtc
    13921 gccgaagctg atccgtgact tcaagctgtt cgcggtgctc gacgacgaca aggccgacac
    13981 gctggagatc accgcgatcc acgacgatga cggctaccgc aaggtgcgcc agtcgctgtc
    14041 ggaccagtac aacctcggct cgcgcgagcc gaacatccag gtctggaacg tcgaccgccg
    14101 cggcgaccgt tcgctgacgc tgcgccacag cgtctacaac cgccggccgc tgaacgccgg
    14161 gaccacgtac gaggtgctca agcacatgtt ccggctgtgg ggcttcacgg tgcggctcga
    14221 agcggtcgac gagaacggca aggtcagcct cgtcaacgag gtgcgcaagc actgagccgg
    14281 acgcacgccc tcccgccctg ccgccgacgc cgcgccgagt gcgcggcgtc gccttgttgc
    14341 accggcgccg atcttccgta gactcgcgcc ccgccccgcc gaggactgtc cgcccctcat
    14401 gctgcgtccg ctgctgtcgt ccctgttgct cgtccctgcc ctcgccgctg ccggcccctg
    14461 gggcgaagtc cgcctgccga gtgccgagct gccgcaggtc tacggcggtt atgccgccgg
    14521 ctgcatcgcc ggcgcgcaga cgctgccgct gtccggcccc ggctatcagg cgatgcgccc
    14581 gtcgcgcaac cgctactacg ggcatccgga gctgctgcgc ttcatcgaac agctcggcta
    14641 cacggccgcg gcccgccgct tcgcgccggt gctgatcggt gatctcggcc agccgcgcgg
    14701 cgggccgatg ctctcgggtc accgcagcca ccagatcggc ctcgatgtcg atgtctggtt
    14761 cctcgacgcg cccggtggcc gcgcaccgtc cggagcagcc ctcgaaagcc tgagcgcgca
    14821 gacgatgatc gacccgtacg ccgggcgcgt gctcgcgcgc ttcggtgccc gccagcgcgc
    14881 actgctgcgc gcggcggccg acgatccggc ggtcgagcgc atcttcgtca atccggtgat
    14941 caagcagagc ctctgccgca gcgaggccga tcgcggctgg ctgcacaaga tccggccgtg
    15001 gtggggacac gatgcgcact tccacgtgcg cctgtactgc ccgccggaca gcccgcaatg
    15061 ctcggcgcag aagccgatcg aacccggtga cggctgcgat gccgggctcg atgcctgggt
    15121 cgaggaaatc cgtcaggccg cgcgccgccc gccgctgccg acgccgccgg cacccgaacc
    15181 ggtgctgccg gaggcctgca cggcggtgct gcaggcaccg tcggcaagcg cgaggcgctg
    15241 atgcgaggcc ggctgaagct cgtgctgctc gcgagcctcg ccgccggcgg ctgcgcgtcg
    15301 ctgccggcgg gtctgacgcc gacctcggcc gagcatgc
//
LOCUS       DAOQIE010000047        15060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1888_length_15060_cov_6.866045, whole genome shotgun sequence.
ACCESSION   DAOQIE010000047 DAOQIE010000000
VERSION     DAOQIE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15060
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_15060_cov_6.866045"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2785)
                     /locus_tag="PK144_04715"
     CDS             complement(<1..2785)
                     /locus_tag="PK144_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65064.1"
                     /translation="MSEVPPAETAADTAAAPPPAPPAPPRRRSRWLRALGWFFGGLLA
                     LLLILLGALGWLLGTDSGLQALIGLARDHAPGTLVVGKAEGRVLGDLRLSGFDWRNGE
                     LHVQVARLELDWSPRRLLEPALHISRLAVSGVALKLPPPAPPPEPPPPAQPFTLPNLA
                     LPLAIELDNVMVDGVRVQPAAGAPVVIDSARLAARYTVDGLTLDHFDVRMPGMNAGGK
                     GHLAPAGRWPTTIELGGHLDETPLAAPLDITLQLAGTLDRFELKASTRGAVESTVTAS
                     VDDALGLPRWAAEVQALAVLQALAPELGTPAWQLALEAAGTTTEIRVRSLAVHRAGSR
                     LALTALGGISLAPDPAMSRIEFEGDWRALAWPIDGPAQIASPEGHFRVSGTQADWRTT
                     LKLLADVPEAGRLATEASVRGDTAHAVIESLAVRSPDGPAQIDASARVSFADLRFAVD
                     ANWRQLGWPLKGKAQYASPKGRLQAEGTPKQYTYALELDAAGDSVPPLTLKASGDGSD
                     EAARLATLAITLLDGRIDGRAGLRWAPRLEWEAELAGQKLDPGRFAPEWPGRIAFELT
                     SDGHIDADGQPQAHAVLSKLDGRLREQPLDGRIDARVSGANLDLPAAKLVYGGTRLDA
                     EGRIHETWDLRWKLAAPDLARAAPGLRGRLSGDGQLSGPRERPQLQAKLDGQNLGWPD
                     GGLQTLTVRADVDSAGRRRSELHVDGRALALAGQSFERLQLDADGTAAAHRAELTLNG
                     APVRAALRLSGRIDGEQWLGRLERLDLLQTGLGDWKLEQPASIRLATAFDAAKVSPLC
                     LASRPTRLCVQADWRAADGGTATLKLTKFVPDKVKKFLPPELALQTSVDADAEAVLGP
                     GPGHALKNARALVTIAPGRVRWTGAAAPIELPVNGRLEAKLDAGSQLDGRLQFGLGGS
                     DGIDAEVRTSGLTA"
     gene            complement(3159..4931)
                     /locus_tag="PK144_04720"
     CDS             complement(3159..4931)
                     /locus_tag="PK144_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNF65065.1"
                     /translation="MHHTTPGWRRVAIALLLTGSAATAHGQASTPGKTGVPVEVVIEG
                     IEDERLRVNVANFLSIRELASKPEATDSRIAWEHRKAEEEIRTALQPFGYYRPTIQAS
                     LERDGPGWRATYRVTPGTPVRVRTLDIQLSGAATGDASFHKLLKNLPIHVGEAFEHEH
                     WEKLKSGITGLITEHGYFSAQIVMSEVRVDPAAGSADVHLHVDSGRRYRFGETTFHQT
                     ILKDEVLRRYNRIKPGDPYDAAALIDLQGALADADYYQSVEIEAGPDRAEGDTIPLDV
                     RLRPRLRDRWDFGLGYGTDTGPRGRIGFKERWVNDLGHKFTSDLRVSQIKTTLAAEYL
                     IPGNDPRLEQYSIHSRADYEDSTTVENRLFLIGVGQKLQAGDWQRLTALDWQIERFRY
                     FDTPADIMLLIPSVTFSRSTVDDSLRVYRGERLSLQLRGALEPILSDNTFFQVTAGVK
                     EVWPLGKDMRLLARFDAGTTWTSDPEQLPSSVRYYAGGDTSIRGYEYQSIGPRNDRDK
                     VIGGRHMLVGSIELEHRVVGDWSVAAFVDAGDAFERSPSMKLGAGIGVRWASPIGPIR
                     LDLAHGFDEPGDAFRIHFTLGPDL"
     gene            complement(4937..6496)
                     /locus_tag="PK144_04725"
     CDS             complement(4937..6496)
                     /locus_tag="PK144_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF65066.1"
                     /translation="MSPDTTFDTLARSLANPDLYPGADTVRRLDTHISAVFLAGDRAL
                     KIKKPLNLGFLDFGTLQRRAHFCREELRLNRRLAPDIYLDCIPICGSPENPVLGGDPA
                     EAIEWAVSMRRFAQENLLDRCLAEGRLTAAHIDAMARSLAGFHAGIAVAGDDSPFGTP
                     EAVAAPAFDNFRHARELLHAPEDRAALDRIEAWTQATHDRIGPLLAARKAGGRIRECH
                     GDLHLGNMILDGDAVTVFDCIEFNDAFRWIDVAADLAFVVMDLQQRGRDDLAARALNV
                     WLEYSGDHDAAGVLPFYLVYRAMVRAKVAAIRLGQADVPPDEAAAVLAEARRYLALAE
                     RYTQPPQPFLLITHGVSGSGKSTATQTLLEAFGAIRLRSDVIRKQLFGLGPLDDSHAA
                     GSDIYTQKASERTYARLLELAAQLLAQGWPVLVDATFPQAWQRAPFRERAQQAGVPFV
                     LLACSAEADTLRARVAARRQAGTDAAEADVRVLDAQLAGYVAPDADEQPLSAAGLDAA
                     ALTAAVRARLG"
     gene            6639..9083
                     /gene="mrcB"
                     /locus_tag="PK144_04730"
     CDS             6639..9083
                     /gene="mrcB"
                     /locus_tag="PK144_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HNF65067.1"
                     /translation="MKKPEAPRKRRPSFVRGLFGLIGLCLLAATLVGAVLLYNYASHL
                     DGVIRTQFEGKRWALPARVYARPLEAFVGATLNAEQLQHELDLLRYRRADSIDGPGLY
                     RRNGDTLELSTRGFAFWDGTEASRHVRVGFRDGTLVGLDGLDGQPSPDVLRLEPFEIA
                     GIYPAHHEDRVLVRRQDLPKPLVDALVATEDRTFFEHFGIDLKGVARAAIANLRAGRT
                     VQGASTLTQQLVKNFFLSNERTLTRKLNEMLMAVLIDAHYSKDDILEAYSNEIYLGQD
                     GARAIHGFGRAARFYFDRPLGELTLPQMALMVGLVKGPSYYDPRRHPERAKERRAIVL
                     EAMTELGYIDAVAAAEAKAAPLGVVERQTNGLTLYPNFVDLVQRQLRESYREEDLTSE
                     GLRIFTTLDPRMQDIAEQTLLASLPVLEKSQRMKPDTLQGAVVLASPDNGEVYALVGG
                     RDVRLAGFNRVLDARRPAGSLLKPAVYLAALEDSEHYSLASSLHDEPFTWRIPGGGGD
                     WSPHNYDKRYRGRVMLRDALAKSMNVPTAWIAQQIGMERVVDIMKRLGARQNLRTNPS
                     LVLGAADLTPFEIAQMYQTLASGGFRAPLRAIREVTTKDGKPLSRYPLDIQQVVKPGP
                     AYLIINAMQAVVREGTAAGLAGKAFDRKLGLAGKTGTTDDLRDSWFAGFSGNLMALVW
                     VGRDDNQPTGLSGATGALRTWVDLMKQLPLTPLDPAPPLEVATIAVDRASGGLADRNC
                     HNTLMLPFLAGSEPRALAPCHAGYVPPEGYAGGGMDTNGDTGSEEPVAGGSAEPRTAA
                     PSNNDSIGDFFRRLMQ"
     gene            9080..9766
                     /locus_tag="PK144_04735"
     CDS             9080..9766
                     /locus_tag="PK144_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65068.1"
                     /translation="MTTLPRPPAPWRLAPLLALLLAAGCTAPSPFGAPVEDRTRAPAA
                     SRTRPLPPPAPGVEVMPLDAPAPLTPTEPLVGQPLPAAPAAPATPPPPRPAPAPAPVA
                     SAPAAPSAPAEPEAERGGNNAVVALIDSANTYVRNNELDKAAASLERAQRLEPRNPNI
                     LYDLAQVRAHQAQYGQSEALAQKSIALAGGNKALQAKAWRLIAAVRRAGGNAAGGDAA
                     EAQAVTLGGR"
     gene            9872..11710
                     /locus_tag="PK144_04740"
     CDS             9872..11710
                     /locus_tag="PK144_04740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNF65069.1"
                     /translation="MAGAVAAALEDHGTLIAEAGTGTGKTFAYLVPALLSGKRVIVST
                     GTRALQDQLYRKDLPVICAALGSPAKLALLKGRSNYLCDYRLEAAEAAGRFRSREQAA
                     EFARIRHWAAMTRHGDIAEVIDVAEDSPVWPLVTSTADNCLGAECPRLADCKVLQARR
                     EALEADLLVVNHHLFFADMAIREEGFAELLPGADAVIFDEAHQLPEVASSFFGLTLSS
                     RQLVELARDTMIEQGRDARDFPDLAERAAQFEDRVTGLRQALGDEGRRAPWSEVGESA
                     PVIDAIAAITLALSRLLEALKEAAPRGKGLENCHARAVELAQRFAQLTQPPGDEAVHW
                     FETYARSFQIAQTPLDIAATFRGRMAMYQAAWVFTSATLAVGEGFGHYRHRLGLAESA
                     ATLKVDSPFDYARHAVLYHPEGLPEPAAPGYTAALIEAIVPVLEASRGRAFLLFTSYR
                     ALNEAHERLDGRVPYPLLKQGSLPKAALLDAFRRHGNAVLLGTQSFWEGVDVRGEALS
                     CVVIDKLPFSSPGDPVLAARIDAMKRAGGNPFFEYQIPNAVITLKQGVGRLIRDVDDC
                     GVLVLADPRLLTKGYGKLFLDSLPPMTRTRRLDRVQKFFAWAGRER"
     gene            12967..13206
                     /locus_tag="PK144_04745"
     CDS             12967..13206
                     /locus_tag="PK144_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65070.1"
                     /translation="MNLVEKSVVRVIMTGPRWVINNFGLMVLMPILLLSVPCLSRAAE
                     VSFTNVTQAAGIAYIGESWGAHGGISIMMAIWISG"
     gene            13218..>15060
                     /locus_tag="PK144_04750"
     CDS             13218..>15060
                     /locus_tag="PK144_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNF65071.1"
                     /translation="MPTHLYRNNKNGTFTNVASQVLKNYNFDGDNHGAVWVDIDNDGD
                     QDLVELTGAAGGVATTSPNHDANLLVNAGGVLEDRAVEMNFSNPYLRGRMPLPYDFDN
                     DGILEIMQTGDIRSDGLAFPSVWKSNSHGYDDITDQCGAFPGAIAFAQSAAFPQGKTV
                     LLYGGGASPSKAYNIESMPWVDNSAAFLEWQQKSVVVIDAAVADFNNDLNSEFYVARN
                     NQLGIDYAEVKPLGNILFKVTVSPQKGETKKLVFSADGSVDFDLVAPNHNLKQIFIGS
                     AGRNPAQKHFVLSPNDAANQGLPQFSSNVPPGLYVGFDPATHLWSLVNHTPATDRIIG
                     YAVAGNIRATNVAIRDMSSLRTQATKLTDLLFMYDPRQGRHVVTSWGFDASTPTMAVA
                     VADFDNDMDVDIFLLKANNVKNFANQLWLNDGSGHFVAAPDAAGLNMSVQGAGEAVIV
                     ADYDNDGFMDVLITNGYGEPPFNQGPVELFRNSGNANHWLELNLVGGPSNRDAIGARI
                     IATTPDGRKQLREVGEELHNHGQNMRRVHFGLGANDKVASLEIHWPNGEVRTLQDIPA
                     NQILGIMQSMDTDEDSVRDVTDNCPAIANADQVDTDGDGQGDACDEDD"
BASE COUNT         2418 a         4964 c         5229 g         2449 t
ORIGIN      
        1 cggccgtcag gccgctggtg cgcacctcgg catcgatgcc gtcgctgccg ccgaggccga
       61 actgcagcct gccgtcgagc tggctgccgg cgtcgagctt cgcttccagc cggccgttga
      121 ccggcagctc gatcggcgcg gccgcgccgg tccagcgcac gcgccccggt gcgatcgtca
      181 ccagcgcccg cgcgttcttc agggcatgac cggggccggg gccgagcacg gcttcggcgt
      241 cggcgtccac cgaggtctgc agcgcgagtt cgggcggcag gaattttttg accttgtccg
      301 gcacgaactt cgtcagcttc agcgtcgccg tgccgccgtc ggcggcgcgc cagtcggcct
      361 gcacgcagag ccgggtcggg cgcgaggcga ggcagagcgg cgacaccttc gcggcatcga
      421 aggcggtcgc gagccggatc gatgccggct gttcgagctt ccagtcgccg agtccggtct
      481 gcagcaggtc gagccgttcg agcctgccga gccattgttc gccgtcgatg cggccgctca
      541 ggcgcagcgc cgcgcgcacc ggcgcaccgt tgagcgtcag ttcggcgcgg tgcgcggcgg
      601 ccgtgccgtc ggcatcgagc tgcaggcgct cgaaactctg gccggcgagc gccagcgccc
      661 ggccgtcgac gtgcagttcc gagcgccgcc ggccggcgga atcgacatcg gcgcgcacgg
      721 tgagcgtctg caggccgcca tccggccagc cgaggttctg accgtcgagc ttcgcctgga
      781 gctgcggacg ttcgcgcggg ccgctgagct gaccatcgcc gctgagccgg ccgcgcaggc
      841 ccggcgcggc tctggcgaga tcgggcgcgg cgagcttcca gcgcaggtcc caggtctcgt
      901 ggatccggcc ttcggcatcg aggcgcgtgc cgccgtagac gagcttcgcc gccggcaggt
      961 cgaggttcgc gccgctgacg cgtgcatcga tgcgcccgtc gagcggctgc tcgcgcagcc
     1021 ggccgtcgag cttcgagagc accgcgtgcg cctgcggctg gccgtcggca tcgatgtggc
     1081 cgtcgctcgt gagttcgaag gcgatgcgcc ccggccattc gggcgcgaac ctgccgggat
     1141 cgagcttctg tccggcgagt tcggcctccc attcgagccg tggcgcccag cgcagcccgg
     1201 ccctgccgtc gatgcggcca tcgagcagcg tgatcgcgag cgttgccagc ctggccgcct
     1261 cgtcgctgcc gtcgccgctc gccttcagcg tcagcggcgg cacgctgtcg ccggcggcgt
     1321 cgagttcgag cgcatacgtg tactgcttcg gcgtgccctc ggcctggagc ctgcccttcg
     1381 ggcttgcgta ctgcgccttg cccttcagcg gccagccgag ctggcgccag ttcgcgtcca
     1441 ccgcgaagcg caggtcggcg aagctcacgc gagcgctggc gtcgatctgc gccgggccgt
     1501 ccggcgagcg cacggccagc gattcgatga cggcgtgcgc ggtgtcgccg cgcacgcttg
     1561 cctcggtcgc gagccgcccg gcctcgggca cgtcggcgag cagcttcagc gtcgtgcgcc
     1621 agtcggcctg cgtgccggaa acccggaagt gaccctcggg gctcgcgatc tgcgccggac
     1681 cgtcgatcgg ccaggcgagg gcgcgccagt cgccttcgaa ctcgatgcgg ctcatcgccg
     1741 ggtcgggcgc gagcgagatg ccgccgagcg cggtcagcgc cagccggctg ccggcgcgat
     1801 gcacggcgag cgagcgcacg cggatttcgg tcgtggtgcc ggcggcttcg agcgcgagct
     1861 gccaggccgg cgtgccgagc tcgggcgcga gcgcctgcag caccgctagc gcctgcacct
     1921 cggccgccca gcgcggcagg ccgagcgcat cgtcgacgct ggccgtgacc gtggattcga
     1981 cggcaccgcg cgtgctcgcc ttcagctcga agcgatcgag cgtgccggcg agctgcagcg
     2041 tgatatcaag cggcgcggcg agcggggttt catcgagatg accgccgagt tcgatcgtcg
     2101 tcggccagcg tccggccggg gcgagatggc ccttgccgcc ggcgttcatg ccgggcatgc
     2161 gcacgtcgaa atgatcgagc gtcagtccgt cgacggtgta gcgcgcggcg agccgggcgc
     2221 tgtcgatcac gaccggcgcg ccggccgccg gctgcacgcg cacgccgtcg accatcacgt
     2281 tgtcgagctc gatcgccagc ggcagtgcca gattcggcag cgtgaacggc tgcgcgggcg
     2341 gcgggggttc cggcggtggc gccggcggcg gcagcttcag cgcgacgccg ctgacggcga
     2401 gccggctgat gtgcagcgcc ggttcgagca gccggcgcgg cgaccagtcg agttcgaggc
     2461 gcgcgacctg cacgtgcagc tcgccgttgc gccagtcgaa gccgctcagg cgcagatcac
     2521 cgagcacgcg gccctcggcc ttcccgacca ccagcgtgcc cggcgcatga tcccgcgcga
     2581 ggccgatcag cgcctgcagg ccggaatcgg tgccgagcag ccagccgagc gcaccgagca
     2641 ggatcagcag cagcgccagc agcccgccga agaaccagcc gagcgcgcgc agccagcgcg
     2701 aacgccggcg gggcggggcg ggcggcgcgg gcggcggggc ggcggcggta tcggcggcgg
     2761 tttcggcggg cgggacttcg ctcatccgtg gacggttcct gcagatacgg gattcgtgga
     2821 aagcagcgac cggactgtgc aggttcctga cttgccgctc acttcacgtc cttcgcctgc
     2881 atggcatcgg tatctgcagg actggcagag gcgggttccg acccggcatc tccggcgcgg
     2941 atggaaccgg acgacggcaa ggtattgacg aacaagcccc tcccccctcg cgggggaggg
     3001 gttggggaga ggggggcaac gcttgggcgc agtgccggcg tttccccctc tcccccggcc
     3061 cctctcccgc aaggggagag gggaggccag gccgatgcct gcggcatgcg gaaaaggatg
     3121 gtccgtgcag ctggccagcg tcgtcagggc agctttcctc acaggtccgg accgagcgtg
     3181 aaatggatgc ggaaggcgtc gcccggctcg tcgaagccgt gcgcgaggtc gaggcggatc
     3241 gggccgatcg gcgaggccca gcgcacgccg atgccggcac cgagcttcat cgacgggctg
     3301 cgctcgaagg catcgccggc atcgacgaag gcggcgacgc tccagtcgcc gaccacgcgg
     3361 tgttcgagct cgatgctgcc gaccagcatg tgccggccgc cgatgacctt gtcgcggtcg
     3421 ttgcgcgggc cgatcgactg gtattcgtag ccgcggatgc tggtgtcgcc gccggcgtag
     3481 tagcgcaccg aggacggcag ctgctcgggg tcggaggtcc aggtcgtgcc ggcgtcgaag
     3541 cgcgcgagca ggcgcatgtc cttgccgagc ggccagactt ccttcacgcc ggcggtgacc
     3601 tggaagaagg tgttgtcgga caggatcggt tcgagcgcgc cgcgcagctg caggctcagg
     3661 cgctcgccgc ggtagacgcg cagcgaatcg tcgaccgtcg agcgcgagaa ggtcaccgac
     3721 gggatcagca gcatgatgtc ggcgggcgtg tcgaagtagc ggaagcgttc gatctgccag
     3781 tcgagcgccg tcagccgctg ccagtcgccg gcctgcagct tctggccgac gccgatcagg
     3841 aacaggcggt tctcgaccgt cgtcgagtcc tcgtagtcgg cgcgcgagtg gatcgagtac
     3901 tgctccaggc gcggatcatt gccggggatc aggtactcgg cggccagcgt cgtcttgatc
     3961 tgcgacacgc gcaggtcgga cgtgaacttg tggccgaggt cgttgaccca gcgctccttg
     4021 aagccgatgc gtccgcgcgg gccggtgtcg gtgccgtagc cgaggccgaa gtcccagcgg
     4081 tcgcgcaggc gcgggcgcag gcgcacgtcg agcgggatcg tgtcgccttc ggcccggtcg
     4141 ggtccggcct cgatctcgac gctctggtag tagtcggcat cggcgagcgc gccctgcagg
     4201 tcgatcagtg ccgcggcatc gtaggggtcg ccgggcttga tgcggttgta acggcgcagg
     4261 acctcgtcct tcaggatggt ctggtggaag gtggtctcgc cgaagcggta gcggcggccg
     4321 gaatcgacgt gcaggtgcac gtcggcgctg ccggcggccg gatcgacgcg cacctcggac
     4381 atcacgatct gcgccgagaa gtagccgtgc tcggtgatca ggccggtgat gccgctcttg
     4441 agcttctccc agtgctcgtg ctcgaaggcc tcgccgacgt ggatcggcag gttcttcagc
     4501 agcttgtgga aggaggcgtc gccggtcgcg gccccggata gctggatgtc gagcgtgcgc
     4561 acgcgcaccg gcgtgcccgg cgtgacccgg tacgtggcgc gccagcccgg gccgtcgcgc
     4621 tccaggctcg cctggatcgt cgggcggtaa tagccgaagg gctgcagcgc ggtgcggatt
     4681 tcttcctcgg ccttgcgatg ctcccaggcg atgcgcgagt cggtggcttc gggcttgctg
     4741 gcgagttcgc ggatgctcag gaagttcgcg acgttgacgc gcaggcgctc gtcctcgatg
     4801 ccttcgatga cgacctcgac cggcacgccg gtcttcccgg gcgtacttgc ctgtccgtgg
     4861 gcagtcgcgg cgctgccggt gagcagcagc gcgatcgcga cccggcgcca tcccggcgtc
     4921 gtgtggtgca tccgggtcag ccgagacgcg cgcgcacggc ggcggtcagc gcggcagcat
     4981 cgaggccggc agcggaaagc ggctgttcat cggcatccgg ggcgacgtag ccggcgagct
     5041 gggcatcgag cacgcgcacg tcggcctcgg cagcgtcggt gccggcctgc cggcgcgccg
     5101 cgacgcgcgc gcgcagcgtg tcggcttcgg cgctgcaggc gagcagcacg aagggcacgc
     5161 cggcctgctg cgcgcgttcg cgaaacggcg cgcgctgcca ggcctgcggg aaggtcgcat
     5221 cgaccagcac cggccagccc tgcgcgagca gctgcgcggc gagctcaagc aggcgcgcgt
     5281 aggtgcgttc gctggccttt tgcgtgtaga tgtcgctgcc ggcggcgtgg ctgtcatcga
     5341 gcgggccgag gccgaacagc tgcttgcgga tcacgtccga gcgcagccgg atcgcgccga
     5401 aggcttcgag cagcgtctgc gtcgccgtcg acttgccgga gccggagacg ccgtgcgtga
     5461 tcagcaggaa gggctgcggc ggctgcgtgt agcgttcggc gagcgcgagg tagcggcgcg
     5521 cttcggcgag cacggccgcg gcctcgtccg gcggcacgtc ggcctgaccg aggcggatcg
     5581 ccgcgacctt ggcgcgcacc atcgcccggt agaccagata gaacggcagc acgccggcgg
     5641 cgtcgtgatc gccgctgtat tcgagccaga cgttcagcgc ccgcgcggcg aggtcgtcgc
     5701 ggccgcgctg ctgcaggtcc atgaccacga aggcgagatc ggcggcgaca tcgatccagc
     5761 ggaaggcatc gttgaactcg atgcagtcga acaccgtcac ggcatcgcca tcgaggatca
     5821 tgttgccgag gtgcaggtcg ccgtggcact cgcggatgcg gccgccggcc ttgcgcgccg
     5881 cgagcagcgg cccgatgcgg tcatgggtgg cctgcgtcca ggcctcgatg cgatcgagcg
     5941 cggcgcggtc ttcgggcgcg tgcagcagct cgcgcgcatg gcggaagttg tcgaaggccg
     6001 gggcggcgac ggcttcgggc gtgccgaacg ggctgtcgtc gccggcgacg gcgatgccgg
     6061 cgtggaagcc ggcgaggctg cgggccatcg catcgatgtg cgcggccgtc agccggcctt
     6121 cggcgaggca gcgatcgagc aggttttcct gcgcgaagcg gcgcatcgac accgcccatt
     6181 cgatggcttc ggccggatca ccgccaagca ccggattttc cgggctgccg cagatcggga
     6241 tacaatcgag gtagatgtcc ggcgcaagcc tgcggttcag ccgcagttcc tcccggcaga
     6301 agtgtgcacg acgctgcagc gtgccgaagt ccaggaagcc gaggttcagc ggcttcttga
     6361 tcttcagtgc ccggtcgccg gcgaggaaca ccgcggaaat atgcgtgtcc agccggcgca
     6421 cggtgtcggc gcccggatag agatccgggt tcgccaggga ccgggccagc gtgtcaaacg
     6481 tggtatcagg gctcatgggg gcttgctggg ctcggcgcga tcggatgcgc atcaggatac
     6541 ctacagaata agccggcctc cggctccaac ccaaccgctg cgccgccgct tgctggcggg
     6601 cgccgagcgc ggcacgtccg cgccctgaac gtgaacgaat gaagaaaccc gaagcccccc
     6661 gcaagcgccg tccgagtttc gtgcgcggcc tgttcggcct gatcggcctc tgcctgctcg
     6721 cggcgacgct ggtcggcgcg gtgctgctgt acaactacgc cagccatctc gacggcgtca
     6781 tccgcacgca gttcgaaggc aagcgctggg cgctgccggc gcgcgtgtac gcccggccgc
     6841 tcgaagcctt cgtcggtgcg acgctgaacg ccgagcagct gcagcacgaa ctcgacctgc
     6901 tgcgctatcg cagggccgac agcatcgacg gtccgggcct gtaccggcgc aacggcgata
     6961 cgctcgaact gagcacgcgc ggcttcgcgt tctgggacgg caccgaggcc tcgcgccacg
     7021 tgcgggtcgg tttccgggat ggcacgctgg tcggcctcga cggcctcgac ggccagcctt
     7081 cgcccgacgt gctgcgcctg gagccgttcg agatcgccgg catctacccg gcacatcacg
     7141 aggaccgcgt gctggtgcgt cgccaggacc tgccgaagcc gctcgtcgat gcgctggtgg
     7201 cgaccgagga ccgcaccttc ttcgagcact tcggcatcga cctcaagggc gtggcgcgcg
     7261 cggcgatcgc caacctgcgc gccggtcgca ccgtgcaggg cgcgagcacg ctgacgcagc
     7321 agctggtcaa gaacttcttc ctctcgaacg agcgcacgct gacgcgcaag ctcaacgaga
     7381 tgctgatggc cgtgctgatc gatgcgcact actcgaagga cgacatcctc gaagcgtatt
     7441 cgaacgagat ctacctcggt caggacggcg cccgcgccat ccacggcttc ggccgcgccg
     7501 cgcgcttcta cttcgaccgg ccgctcggcg agctgacgct gccgcagatg gcgctgatgg
     7561 tcgggctggt caagggcccg tcgtactacg acccgcgccg gcacccggag cgcgccaagg
     7621 aacgccgcgc gatcgtgctc gaagcgatga ccgagctcgg ctacatcgac gccgtcgctg
     7681 cggccgaagc gaaggcggcc ccgctcggcg tggttgagcg ccagaccaac ggcctgacgc
     7741 tgtatccgaa cttcgtcgat ctggtgcagc gccagctgcg cgagagctac cgcgaggagg
     7801 acctgacctc cgagggcctg cgcatcttca cgacgctcga cccgcgcatg caggacatcg
     7861 ccgaacagac gctgctcgcc tcgctgccgg tgctggagaa atcccagcgc atgaagcccg
     7921 acacgctgca gggcgcggtc gtgctcgcga gccccgacaa cggcgaggtc tacgcgctgg
     7981 tcggcgggcg cgacgtgcgt ctggccggct tcaaccgcgt gctcgatgcg cgccgcccgg
     8041 ccggctcgct gctcaagccc gccgtgtatc tggcggccct ggaggattcc gagcactact
     8101 cgctcgcgag cagcctgcac gatgaaccct tcacctggcg cattcccggc ggtggcggtg
     8161 actggtcgcc gcacaactac gacaagcgct accgcggccg cgtgatgctg cgcgacgcgc
     8221 tggccaagtc gatgaacgtg ccgacggcgt ggatcgcgca gcagatcggc atggagcgcg
     8281 tcgtcgacat catgaagcgc ctcggcgccc gccagaacct gcgcaccaac ccgtcgctgg
     8341 tgctcggagc cgccgacctg acgccgttcg agatcgcgca gatgtaccag acgctcgcga
     8401 gcggcggctt ccgtgcgccg ctgcgcgcga tccgcgaggt cacgaccaag gacggcaagc
     8461 cgctcagccg ctatccgctc gacatccagc aggtggtcaa gcccggcccg gcctatctga
     8521 tcatcaacgc catgcaggcc gtcgtgcgtg agggcacggc cgccggcctc gccggcaagg
     8581 ccttcgaccg caaactcggc ctggccggca agaccggcac caccgacgac ctgcgcgatt
     8641 cctggttcgc cggcttcagc ggcaacctga tggcgctggt ctgggtcggg cgcgatgaca
     8701 accagccgac cggcctgtcg ggcgccaccg gcgcgctgcg cacctgggtc gacctgatga
     8761 agcagctgcc gctgacgccg ctcgatccgg caccgccgct ggaggtcgcg acgatcgcgg
     8821 tcgatcgcgc ctccggcgga ctggccgatc gcaactgcca caacaccctg atgctgccgt
     8881 tccttgccgg ctccgaaccg cgtgcgctgg cgccgtgtca tgccggatac gtgccgcccg
     8941 aagggtatgc tggcggcggc atggacacga acggcgacac cggcagcgag gaacccgtcg
     9001 cgggtggcag cgctgaaccg cgcaccgccg ccccgtccaa caacgattcc atcggcgact
     9061 tcttccggag actcatgcaa tgacgacgct gccccggccg ccggcgccct ggcgcctcgc
     9121 tccgctgctg gccttgttgc tggcagccgg ctgtactgct ccctcgccct tcggggctcc
     9181 ggtcgaggac cgcacgcgcg ccccggctgc cagccggaca cgaccgctgc cgccgccggc
     9241 cccgggcgtc gaggtcatgc cgctcgatgc cccggccccg ctgaccccga ccgagccgct
     9301 ggtcggccag ccgctgccgg ctgcgccggc cgcaccggcg actccgccgc cgccccgtcc
     9361 ggcgccggca ccggcgccgg tcgccagtgc tccggctgca ccgtcggccc cggccgaacc
     9421 cgaagccgaa cgcggcggca acaacgccgt cgtcgcgctg atcgattcgg ccaacaccta
     9481 cgtgcgcaac aacgaactcg acaaggccgc cgcctcgctc gaacgcgcgc agcggctgga
     9541 gccgcgcaat ccgaacatcc tgtacgacct tgcgcaggtg cgcgcgcacc aggcgcagta
     9601 cgggcagtcg gaggcgctgg cgcagaagtc gatcgcactc gctggcggca acaaggcgct
     9661 gcaggccaag gcctggcggc tgatcgccgc ggtgcgtcgc gccggcggca acgccgccgg
     9721 cggcgatgcc gccgaagcgc aggccgtcac gctcggcggt cgctgagccg cagccggggg
     9781 ccggaagctg gaactcgatg ccgtcttcgg ccccgatggc ccgctcgcgc gcgcgatccg
     9841 cggctacgcg ccgcgcgtgc agcagcaggc gatggccggg gccgtcgccg ctgcgcttga
     9901 ggatcacggc acgctgatcg ccgaggccgg caccggtacc ggcaagacct tcgcgtacct
     9961 ggtgccggcg cttttgtccg gcaagcgcgt gatcgtctcg accggcacgc gcgcactgca
    10021 ggaccagctc tatcgcaagg acctgccggt gatctgcgcc gcgctcggct cgccggcgaa
    10081 gctcgcgctg ctgaagggcc gctccaatta cctctgcgat taccgtctgg aggccgccga
    10141 ggccgccgga cgcttccgtt cgcgcgaaca ggccgccgag tttgcccgca tccgccactg
    10201 ggcggcgatg acgaggcacg gcgacatcgc cgaggtcatc gacgtggccg aggattcgcc
    10261 ggtctggccg ctggtgacct cgacggccga caactgcctc ggcgccgaat gtccgcggct
    10321 tgccgactgc aaggtgctgc aggcgcggcg cgaggcgctc gaagccgatc tgctggtggt
    10381 caaccaccac ctgttcttcg ccgacatggc gatccgcgag gaaggcttcg ccgagctgct
    10441 gcccggggcc gacgcggtga tcttcgacga ggcgcaccag ctccccgagg tcgcgagcag
    10501 cttcttcggc ctgacgctgt cgagccgcca gctcgtcgag ctggcgcgcg acaccatgat
    10561 cgagcagggg cgcgatgccc gcgatttccc ggatctggcc gagcgcgctg cgcagttcga
    10621 ggaccgggtc accggcctgc gtcaggcgct cggcgacgag ggccggcgcg cgccgtggag
    10681 cgaggtcggc gagagcgcgc cggtcatcga cgccatcgcc gccatcacgc tggcgctgtc
    10741 acgcctgctc gaagccctga aggaagccgc accgcgcggc aagggcctgg agaactgcca
    10801 tgcgcgtgcc gtcgaactcg cgcagcgctt cgcgcagctc acgcagccac ccggcgacga
    10861 ggccgtgcac tggttcgaga cttacgcccg cagcttccag atcgcgcaga ccccgctcga
    10921 catcgccgcg accttccgcg ggcgcatggc gatgtatcag gccgcctggg tgttcacgtc
    10981 ggccacactc gcggtcggcg aaggcttcgg ccactaccgc caccggctcg gtctcgcgga
    11041 atccgccgcg acactcaagg tcgacagccc cttcgactac gcccgccacg ccgtgctcta
    11101 ccaccccgag ggcctgcccg aacccgcggc acccggctac accgcggcgc tgatcgaggc
    11161 gatcgtgccg gtgctcgaag cctcgcgcgg ccgggccttc ctgctgttca cgagctaccg
    11221 cgcgctgaac gaggcccacg aacggctcga cggccgcgtg ccgtatccgc tgctgaagca
    11281 gggcagcctg ccgaaggccg cgctgctcga cgccttccgc cgccacggca acgccgtgct
    11341 gctcggcacg cagagcttct gggagggcgt cgacgtgcgc ggcgaggcgc tgtcctgcgt
    11401 cgtcatcgac aagctgccgt tttccagccc cggcgacccg gtgctggccg cgcgcatcga
    11461 cgcgatgaag cgcgccggcg gcaacccgtt cttcgagtac cagatcccga acgccgtgat
    11521 cacgctcaag cagggcgtcg gccgcctgat ccgcgatgtc gacgactgcg gcgtgctggt
    11581 gctcgccgat ccgcggctgc tgaccaaggg ctacggcaag ctgttcctcg acagcctgcc
    11641 gccgatgacg cgcacgcgcc ggctcgaccg ggtgcagaag ttctttgcgt gggcaggacg
    11701 tgagcgatag ctgaaaacgg cccggcccgg acttcggagc gccgggcagg aagtcctgca
    11761 ggccggacag cctgcaggcg gctccaggcg cgccgaagac ctgccccacc tgcaggcgcc
    11821 cgacccccgg ggcgcccggc ggtgactgag ggctgtcagc tgcgcccctg atccttcggt
    11881 actgaaatcg gtgtcactcg accggcgcca cgccgtcctg gcggggattg acctgtcggt
    11941 gccggctcga ccgggtgcat aagttcttcg catgggcaag acaacagcga cccggcgaat
    12001 aagcccggat ggcgccgctt caactttggg aatacggcga ttaacccatg caatttgcgc
    12061 atgcctttgc gccgatgatc cggctttgca cgggtggcac tgagttggcc gctatctgca
    12121 tgacccatgt ccgccatatc ggatgaggct ggccggacat caaatcgacc aaaattaaat
    12181 atcctttatg ttcaatgaat taagtcgtga tgacgttgga tttggattta tcatgccgca
    12241 tgcacggctg ctgcggcagc agaatcaaaa accaagttcc gcgcttgggt aaatgcctct
    12301 tctcatcatg cagaaatgag aaggggcgtc ctgacgaaga accgcggtga tgtgcgattg
    12361 atcatggcgc cagaagcccg cctgagtcac gatcaacttt catgaaggaa gatatcgggg
    12421 cgacaggctt ttcgtattac aaaaatgacc aagcttttct tctatggaca aatttgtctc
    12481 atgggtgtaa taggggtacg ctattgtctc atgagagatg ggagccgggt tgcagttccc
    12541 tgagacggta aagacggagg catccgcccg gttcccgatg ccagacgtgc acgagggggc
    12601 agggcattga caggcttctg atatcaggtg caggggatgg attcggatcg cagtccggtt
    12661 ctcaccttga atgcccatgg gcaggggcgt tccaaggggt aacgattaaa accggcaaac
    12721 tgccatccat cagcccatgt gagcgaactg ttcgtggacg gattcgtcat gccgaataaa
    12781 ttggatcagt cagttgccgg cgattgagca ccgcttcctg aaagccctga tcattgccgt
    12841 catggttttg catcgtcagg agatgggcct gccttgtttt gacacggact cctggaccgg
    12901 gagttatgca gcaattctgg atggtttgtg caagtcagtt tttgaaagtt acaggggatg
    12961 gacgcaatga atctcgtaga gaagtccgtt gtgcgggtca tcatgaccgg acccagatgg
    13021 gtcatcaata atttcgggtt gatggtgctg atgccgatcc tgctgctgtc cgtgccctgc
    13081 ttgtcgcggg ctgcagaagt cagtttcacg aatgtcacgc aagcagccgg cattgcatat
    13141 atcggtgaaa gctggggggc gcatgggggg atttcaataa tgatggctat ctggatatct
    13201 ggctgacaaa tcacagaatg ccgactcatc tgtatcgcaa caataaaaac ggcacgttca
    13261 cgaacgtcgc ctcgcaggtg ctgaagaatt acaattttga tggtgataac catggcgcgg
    13321 tatgggtgga tatcgacaat gatggcgatc aggatctggt ggaactgacc ggcgctgccg
    13381 gcggtgtggc aaccaccagt ccgaatcatg atgccaatct gctggtcaat gccggcggcg
    13441 tgcttgaaga tcgcgccgtg gaaatgaatt tcagcaatcc gtatctgcgt ggcagaatgc
    13501 cgctcccgta tgatttcgat aatgacggaa ttctggagat catgcagacc ggggacatcc
    13561 ggtcggatgg tctggcattt cccagtgtgt ggaaaagcaa ttcgcatggg tacgatgata
    13621 ttacggacca gtgcggtgcg tttccggggg cgatcgcatt tgcgcagagt gccgcgtttc
    13681 cccaagggaa aacggttctg ctgtatggag gcggagcaag tccgtcaaaa gcatacaaca
    13741 tagaatccat gccgtgggtg gataactctg cagcgttcct tgagtggcag cagaagagtg
    13801 tcgtggtgat cgatgcggcg gtcgcggatt tcaacaatga tttgaacagc gagttctatg
    13861 ttgccagaaa taatcagctt ggtatcgatt atgccgaagt gaagccgctt gggaacatac
    13921 tgttcaaggt tactgtttct ccacagaagg gtgaaacaaa gaaactggtg ttttctgcag
    13981 atggcagcgt cgatttcgat ctggtggctc ctaatcataa tctcaagcag atcttcattg
    14041 gttcggccgg ccggaatccg gctcaaaagc atttcgtgct ttcgccgaat gatgcggcta
    14101 atcagggcct gccgcagttc agcagcaatg tgccgcccgg actctacgtc gggttcgatc
    14161 cagccactca tctctggtcg ctggtgaatc acacgccggc cactgaccgg atcattggct
    14221 atgcggtggc aggaaacatc agggcaacga atgtggccat tcgtgacatg agcagtctgc
    14281 gcacccaggc gacaaagctg acggatttgc tgttcatgta cgatcctcgg caaggccggc
    14341 atgtcgtgac cagctgggga ttcgatgcgt cgacaccgac aatggcagtg gcagtggctg
    14401 atttcgataa tgacatggat gtggacattt ttctgctcaa ggccaacaac gtaaagaatt
    14461 tcgccaatca gttatggctc aatgatggca gcggccattt cgtggccgcc cctgacgccg
    14521 ctggtctgaa catgtccgtg caaggggcgg gggaggcggt gattgtcgct gactatgaca
    14581 acgacggatt catggatgtg ctcatcacca acggctacgg tgaacccccc ttcaatcagg
    14641 ggccggtcga attgttcagg aactccggga atgccaacca ctggctggag ctgaatctgg
    14701 ttggcgggcc gtccaaccgt gatgccatcg gggccagaat cattgccacg acacccgacg
    14761 gacgcaaaca gttgcgggaa gtcggtgagg aactgcacaa ccacggccag aacatgcggc
    14821 gtgtgcattt cgggctcggt gccaatgaca aggttgcttc gctggaaata cactggccga
    14881 acggagaagt ccggactctg caggacattc ccgccaatca gatcctgggc atcatgcaat
    14941 ctatggatac ggatgaggac agcgtcaggg atgtaacgga taactgtccg gcgattgcca
    15001 atgctgatca ggtcgatacg gatggcgatg gccaggggga tgcctgtgat gaggatgatg
//
LOCUS       DAOQIE010000048        15028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1893_length_15028_cov_7.511521, whole genome shotgun sequence.
ACCESSION   DAOQIE010000048 DAOQIE010000000
VERSION     DAOQIE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1893_length_15028_cov_7.511521"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PK144_04755"
     CDS             complement(<1..1888)
                     /locus_tag="PK144_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNF65072.1"
                     /translation="MTDTPRLTIRRAAVLGAGVMGAQIAAHLANADVPVLLYDLAASD
                     GPPEGIARAAIARLGRLEPAPLATPGRAAHIEPASYAHDLARLGDCDLVIEAIAEKLE
                     WKTALYAQVAPHLRADAVFASNTSGLSIATLAAALPAGLRARFCGMHFFNPPRYLPLV
                     ELIRHPDTDPQLLDALETWLTSWLGKHVLRAPDTPNFIANRVGVFSMLAVLRHTQTFG
                     LGLDEVDALTGPAIGRPKSATYRTADVVGLDTLAHVVDTLRSTLPEDPWRASFTLPDW
                     LQALIARGALGQKTGAGIYRKDGRVIRVLDPASADYRDSRGTVAAEVAAILAGRDPVA
                     RFAALRASAHPQARFLWAIFRDLFHYCAVHLADIADSARDIDCALRWGFGWALGPFET
                     WQAAGWRAVAEAIEADRVAGRTLSDAPLPAWVPALEGVHTPAGSYSPRRGSFAPRSTL
                     AVYARQLCPERLLGEAAPEAGETLFENAGARLWRRPDIDARIGVLSFTSKMHALTEPV
                     LAAIHAALDLAEAQLDGLVLWQRPPFAVGADLRQMRAEVMAVEDGGDPQRLDDIVAFF
                     QRTALRLKYARVPVVAAIDGLALGGGCEFAMHVAHRVFALEAPIGLVEAGVGLIPAGG
                     GCKEF"
     gene            complement(1960..2460)
                     /locus_tag="PK144_04760"
     CDS             complement(1960..2460)
                     /locus_tag="PK144_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNF65073.1"
                     /translation="MPYDPKFMQRALALSATSLDTPGTQPFGAVVVRHGEIVGEGLNH
                     ALARLDPTAHGEIEALRDACRRLGRLDLGDCELYTSCEPCALCVAALQMAGIRHLYYA
                     AGLRACTALFEPLAATRWRNLDTDDLRVQSGLPGGARRLPDSQHEADAALRIIAVWVD
                     ARVAVP"
     gene            complement(2502..2858)
                     /locus_tag="PK144_04765"
     CDS             complement(2502..2858)
                     /locus_tag="PK144_04765"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNF65074.1"
                     /translation="MPGLSIHVVDVAGGRVAQGMRVEVRYAEGEGCLQPLADGPVSAS
                     GLLEDERLARRFAPGRVQVRFHVAHWYRQAGSVLPQPPFLDVVVFDFGLADPGAHYHL
                     PFKCTPWGYSCFRGGA"
     gene            complement(2877..3728)
                     /locus_tag="PK144_04770"
     CDS             complement(2877..3728)
                     /locus_tag="PK144_04770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF65075.1"
                     /translation="MSEQTLVLPAPPLPRRAAPSFEAWSLRCVFGVLAALALVFLVAP
                     TLVILLTSFTATESLRFPPEGFSLRWYAALLDADQMQAAAWNSLVVALWTTVLCVLLG
                     TPAAIAIARSKSPWVRACDVLFMSPLLLPALAFGFAALIFIHRLGLRPDLPLLVLGHV
                     IVCTPFVLRTTIAALTQLEPSLLDASRSLGASEWTTFRRVTLPLIARGIGAGAFLAFM
                     ASFDNVPVSLFLADERTEVLPIHLWQQIENNLDVRTAAASGLIVLLTLTLMVIAERTA
                     GLTRQMR"
     gene            complement(3721..4554)
                     /locus_tag="PK144_04775"
     CDS             complement(3721..4554)
                     /locus_tag="PK144_04775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF65076.1"
                     /translation="MAAPRAPLTLPLTALFAAGFVAPLCVLLMLSLAAGDDGSAGLTL
                     AQYARFLGDRFNLSVLGSTLLLGAEATLVCLLFGFPVAWVCARAGARWQSVLIFLVVL
                     PILTSVVVRTFSWIVILGRQGLLNQILLGLGLIDAPLKLLYSEAGVVLVLAQVQMPLM
                     VLPILTVLSRLDPNLADASRALGAGEWRTLWRVTLPLVMPGIIAGCILSFAASITAFV
                     TQTLIGGARLLYLPLFIYQQAVGANDWPFAAAVSVVFMIAVLLVVVLLNGFGRRYEKH
                     V"
     gene            complement(4557..5615)
                     /locus_tag="PK144_04780"
     CDS             complement(4557..5615)
                     /locus_tag="PK144_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNF65077.1"
                     /translation="MNERFLMTRRELLMAGGALALGALLPAVRARAEAGKLVAATYPG
                     TWSEAHRNVLKPAFQKRVDAGVTQSIILGTDQVARLAAAKGQTPPFDVAIFDPPQVLD
                     AVRQGLIAEYPAAQSPHYAELLPVYQDRWGPKVSMQVIGLAYNPERIKTPPKRWDDLW
                     DPQYRGRVGMTALNSQLGLAFLAELNRLRGGSSSDFEPAFRALRELLPALGAVAANLG
                     AFATLWQQEQIDIAPYNFNFVQTLKAKGVPVEFVVPETGAVAWHTSLHVVAGTTVPEL
                     AVQYIDTHLDPLVQAAMMQPPYDVIPTNGKVALAGAVTQSVAKTQEALAALRGVDWAV
                     LTEQRSALIERFNREIRV"
     gene            complement(5765..6856)
                     /locus_tag="PK144_04785"
     CDS             complement(5765..6856)
                     /locus_tag="PK144_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65078.1"
                     /translation="MSGQRGHELQLEAITQRFHDFVAVRDINLRIGAGELVALLGPSG
                     CGKSTLLRIISGFMRQTEGRVLFDGEPVDHLSPSRRGVGIVFQNYALFPHLSIRQNIA
                     YGLEVQGVAKAKRDARVDEMLALVHMSEFAGRFPRQLSGGQQQRVALARCLAVDPKIL
                     LLDEPFGALDKNLRLDMQIEVKRLQREYGITTVLVTHDQEEALSMADRVAVMSRGRVE
                     QIASPTDIYDRPATLFVNQFVGSTNVLDGTVSTADADGSPVVALAGGRRIRVASDAPL
                     TAGSAVRVSIRPEHLHLHAEAPGADALPGAVQTALPLGALVVYDLALEGGAALKVSVP
                     RQRGEALLPAGAQVWAAPQPSAGYRAFPA"
     gene            complement(6862..7749)
                     /gene="uraD"
                     /locus_tag="PK144_04790"
     CDS             complement(6862..7749)
                     /gene="uraD"
                     /locus_tag="PK144_04790"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNF65079.1"
                     /translation="MDIPIPDRDAAAQLEALNACPEDAFVAALGEVVEHSPWVVRRVA
                     ALRPFASVWALHAALAGVLRASDADTRTALLQRHPELAGREAGEGTLTADSNREQSRL
                     GLDRLSAAEHARLRALNTAYRERFGFPFITAVRLHADLASIFAEGERRLANAAATEAG
                     VALTQVAEVVRGRLAQRFGIAPGWLSTHVLDAVAGLPAAGVALTLSVQHAGGWRRLGG
                     FETDAEGRCPLLLCDDAMRAGTYQIEFRIGDYFRSRGHDGGGFLDTVPVRFVVSDAAL
                     HYHVPLLCTPASYTTYRGS"
     gene            7863..8810
                     /locus_tag="PK144_04795"
     CDS             7863..8810
                     /locus_tag="PK144_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF65080.1"
                     /translation="MPQSLLPKMLRYAEVVARAGSVQAAARVLNLSASAIDRQILLLE
                     DLLGAPLFERQPDGMRLSAAGELVVTLAKRWQHDASRLLTDIRQLQGINQGSLRLAAM
                     DSHANGLMPVFLERLHREHPRIRLEIDIVSPDQAEAALIAGTTDLAIAFNLKAGRDLH
                     LVMQAELPLGCVVAPGHPLAGQPDTSLKAVSGYPIVSQNRSLTIRRYLESRHGWLFAE
                     REAPVQTNSLQLVKHLAAAGSHVALTSELDAAPEILAGRLCFIPLTDRNVQPQTVAIA
                     ISARRPLPRIGRLVADLLAEELRGCLEAVRQRRAAGSGG"
     gene            complement(8888..8978)
                     /locus_tag="PK144_04800"
     tRNA            complement(8888..8978)
                     /locus_tag="PK144_04800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8942..8944),aa:Ser,seq:gga)
     gene            complement(9085..11166)
                     /gene="pnp"
                     /locus_tag="PK144_04805"
     CDS             complement(9085..11166)
                     /gene="pnp"
                     /locus_tag="PK144_04805"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNF65081.1"
                     /translation="MASVKKSFQYGQHTVTLETGEIARQADGAVMVSMADTVVLVTAV
                     GAKEAAPGRDFFPLTVDYQERTYAAGRIPGGFFRREGRPSEKETLTSRLIDRPLRPLF
                     PEGFYNEVQIIATVMSANNDIDPDIPALIGASAAVALTGMPFGGPIGAARVGYINGQY
                     LLNPSHSELKTSQLDLVVAGTENAVLMVESEAQELSEEVMLGAVVFGHEQMQAAIRAI
                     RELAAEAGKPAWDWSAPAADTALDAAVRSAAFGSLVEAYQIAVKQDRQNRVREIRGSV
                     VEQLAGEGAAYTPEAVKTAYSNLEYRVVRDRILDGNPRIDGRDTKTVRPITVRAGVLP
                     RTHGSALFTRGETQALVVATLGTTRDSQIIDALEGEYRELFMLHYNFPPFSVGETGRV
                     GSPKRREIGHGRLAKRGVAAVMPDMEKFPYVVRVVSEITESNGSSSMASVCGSSLALM
                     DAGVPLKAPVAGIAMGLIKEGERFAVLSDILGDEDHLGDMDFKVAGTTQGVTALQMDI
                     KIDGITKEIMQVALAQAREGRLHILGCMNAVLDRPRDDISSFAPRIVTIKINPDKIRD
                     VIGKGGAVIRQLTEETGTTIDIDDDGTVKIAAVDKSASDEARRRIEMITADIEVGRIY
                     EGKVVRIMDFGAFVSLLPGRDGMVHISQICEERIQSVTDKLAVGDSVRVKVMEIDKQG
                     RVRLTMRQVDA"
     gene            complement(11228..11497)
                     /gene="rpsO"
                     /locus_tag="PK144_04810"
     CDS             complement(11228..11497)
                     /gene="rpsO"
                     /locus_tag="PK144_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013333888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNF65082.1"
                     /translation="MSLSNEQKAQVVQEFQRAANDTGSPEVQVALLTARINGLQPHFQ
                     SNKQDHHSRQGLLKMVNQRRKLLDYLKRGDLGRYQDLIARLGLRR"
     gene            complement(11672..12601)
                     /gene="truB"
                     /locus_tag="PK144_04815"
     CDS             complement(11672..12601)
                     /gene="truB"
                     /locus_tag="PK144_04815"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNF65083.1"
                     /translation="MSSRETQPRVRRERRDVHGILLLDKPHGLSSNAALQAVKWLYAA
                     RKAGHGGSLDPLATGLLPCLFGEATKVSGLLLDAGKRYRFTAKLGVTTTTGDAEGALL
                     AERPLPPIDAGVLETVFTRFRGPIEQVPPMHSALKRDGRPLYELARQGIEVERAPRSV
                     TIHSLQLLDWSAGSFSAEVACSKGTYIRVLAEDIGAALGCGAHLLELRRIGVEPWDAA
                     RMLTLDQLRALGEDRDALDACLLPIDSALAGWPRVELGDEAAHGFVNGNPVRANPLPV
                     DGEVRVYGNGRLLGIGCPTGDGRVAPRRVLNAS"
     gene            complement(12598..13005)
                     /gene="rbfA"
                     /locus_tag="PK144_04820"
     CDS             complement(12598..13005)
                     /gene="rbfA"
                     /locus_tag="PK144_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNF65084.1"
                     /translation="MAKEFSRTRRLGEQVRRDLAALMRSEIDDRRVAMVSITQVEISR
                     DLAHAKVWVTYLGPEAERPAVLKQLAEHAGLLRQQLGRGLHVRTVPRLAFVYDESIER
                     GAKLDALIRQAVAEDAARHPDDADDHPGDDDPA"
     gene            complement(13027..>15028)
                     /gene="infB"
                     /locus_tag="PK144_04825"
     CDS             complement(13027..>15028)
                     /gene="infB"
                     /locus_tag="PK144_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012143723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNF65085.1"
                     /translation="EDRPRRPAGADAAPAAAPAAPATADNTRRRGSDDRGRRRDDEPR
                     GKGGDFDGDRRGKGRKGGKGGRLVGAASKQGFVKPVSPMTREVALPETITVAELAAKM
                     AVKATEVIKTLMKLGTMVTINQVLDQETAAIVVEEMGHSYKLLSENALEDELAAEMGV
                     VHEARSRAPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYHVETGKGTIT
                     FLDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTVEAIQHARAAGVPIVVAVNK
                     MDKNGIDLERVKSELSQHGVISEEWGGENLFAYVSAKSGQGIDGLLDQILVQAEVLEL
                     TAAVDGPAKGVVIESRLDKGRGPVATLLVQSGTLRKGDIILTGLEYGRVRAMLDETGR
                     PIDEAGPSIPIEVLGLSGAPNAGDEAVVVSDERKAREIALFRQGKFREVKLARQQKAK
                     LENVFSQMAEGEIATLNVVLKTDVQGSAEALSEALTKLSTDEVRVRIISSGVGAINES
                     DANLAVASEAVMIGFNVRADSAAKKVIEEEGVDLHYYSVIYDVIDEIKKALVGMLSPE
                     VKEQIIGLVQVRQVFRHPKFGAIAGCMVVDGVVKRSAPIRVLRENVVIFEGELDSLRR
                     FKDDVAEVRQGFECGIGVRNYNDVREGDQIEVFERIEVQRTL"
BASE COUNT         2285 a         5251 c         5300 g         2192 t
ORIGIN      
        1 cgaattcctt gcagccgccg ccggccggaa tcaggccgac gccggcctcg accagcccga
       61 tcggcgcttc gagcgcgaac acgcggtgcg cgacgtgcat cgcgaactcg cagccgccgc
      121 cgagcgcgag gccgtcgatc gccgcgacca ccggcacgcg cgcgtacttc aggcgcagcg
      181 ccgtgcgctg gaaaaacgcg acgatgtcgt cgagccgctg cggatcacca ccgtcctcga
      241 cggccatgac ctcggcgcgc atctgccgga gatccgcccc gaccgcgaac ggcggccgct
      301 gccacagcac caggccatcg agctgcgcct cggcgagatc gagcgcggcg tggatcgcgg
      361 cgagcaccgg ctcggtcagc gcgtgcatct tcgacgtgaa cgacagcacg ccgatgcgcg
      421 catcgatgtc cggccgccgc cacaggcgcg cgccggcgtt ctcgaacagc gtctcgccgg
      481 cctcgggcgc ggcctcgccg agcaggcgct ccgggcagag ctggcgggcg tagaccgcca
      541 gcgtcgaacg cggcgcgaag ctcccgcggc gcggcgaata cgaacctgcc ggcgtgtgca
      601 cgccttcgag cgccggcacc caggccggca gcggcgcgtc cgacagggtt cgcccggcga
      661 cgcgatcggc ctcgatcgcc tcggcgaccg cgcgccagcc ggccgcctgc caggtctcga
      721 acggcccgag cgcccagccg aagccccagc gcagcgcgca gtcgatgtcg cgggcgctgt
      781 cggcgatgtc ggccagatgc acggcgcagt aatggaacag gtcgcggaag atcgcccaca
      841 gaaaccgcgc ctgcggatgc gcgctggccc gcagcgctgc gaaacgcgcc accggatcgc
      901 gcccggcgag gatggctgcg acctcggcgg cgaccgtgcc gcggctgtcg cggtagtccg
      961 cactcgccgg atcgagcacg cggatcaccc tgccgtcctt gcggtagatg ccggcgccgg
     1021 tcttctggcc gagggcgccg cgggcgatca gcgcctgcag ccagtcgggc agcgtgaagc
     1081 tcgcccgcca cggatcctcc ggcagcgtcg agcgcagcgt gtcgacgacg tgcgcgagcg
     1141 tgtcgagccc gaccacatcg gcggtgcggt acgtcgcgct cttcggccgg ccgatcgccg
     1201 gtccggtcag cgcatcgacc tcatcgagcc cgagcccgaa ggtctgcgtg tgccgcagca
     1261 ccgccagcat cgagaacacg ccgacgcggt tggcgatgaa gttcggcgta tcgggcgcgc
     1321 gcagcacgtg cttgccgagc cagctcgtca gccaggtttc gagcgcatcg agcagttgcg
     1381 ggtcggtgtc cggatgccgg atcagctcga ccagcggcag gtagcgcggc ggattgaaga
     1441 agtgcatgcc gcagaagcgc gcgcgcagcc ccgccggcag cgccgctgcc agcgtcgcga
     1501 tcgacagccc cgaggtgttc gacgcgaaca ccgcatcggc ccgcagatgc ggcgcgacct
     1561 gcgcgtacag cgccgtcttc cattcgagct tctcggcgat cgcctcgatg accagatcgc
     1621 agtcgccgag gcgcgcgaga tcgtgcgcgt agctcgccgg ctcgatgtgc gccgcgcgcc
     1681 cgggggtggc gagcggcgcc ggttccagcc ggccgagcct cgcgatcgcc gcgcgcgcga
     1741 tgccctcggg cgggccgtcg ctggcggcga ggtcgtacag cagcaccggc acgtcggcat
     1801 tggcgaggtg cgcagcgatc tgcgccccca tgacgccggc cccgagcacg gcggcacggc
     1861 ggatggtcag gcggggggta tcggtcatgc ggcttggccc tgcggcgcga tcagcgcgcc
     1921 ctgcgccggc aagcgtagtc gcgccggccg gcggcttctt caggggacgg ccacacgcgc
     1981 atcgacccag acggcgatga tgcgcagcgc cgcgtcggcc tcgtgctggc tgtccggcag
     2041 ccggcgcgcc ccgcctggca ggccgctctg cacgcgcaga tcatcggtgt cgaggttgcg
     2101 ccagcgcgtc gcggccagcg gctcgaacag cgcggtacag gcgcgcaggc cggcggcgta
     2161 atacagatgc cggatgccgg ccatctgcag cgccgcgacg cacagcgcgc agggctcgca
     2221 gctggtgtac agctcgcagt cgccgagatc gagccgcccg agccgccggc aggcgtcgcg
     2281 cagagcctcg atctcgccgt gcgcggtcgg atcgagccgc gcgagggcat ggttcaggcc
     2341 ctcgccgacg atctcgccgt gccggacgac gacggcaccg aagggctgcg tgccgggggt
     2401 gtcgaggctt gttgcactca gggccagcgc gcgctgcatg aacttcgggt cgtagggcat
     2461 ggcttgcgcc tccgcagagc agatatgtcg ccagccggca ctcacgcccc gccgcggaag
     2521 cacgagtagc cccagggcgt gcacttgaac ggcaggtggt agtgggcccc gggatcggcg
     2581 aggccgaaat cgaagacgac cacgtcgaga aacggcggct gcggcaggac gctgccggcc
     2641 tgccggtacc agtgcgcgac gtggaagcgc acctgcacgc gaccgggcgc gaagcgccgg
     2701 gcgaggcgtt cgtcctccag caggccgctg gccgagaccg gaccgtcggc gagcggctgc
     2761 aggcagccct cgccttcggc gtagcggact tcgacgcgca tgccctgcgc aacccgcccg
     2821 ccggcgacat cgacgacatg gatcgaaaga ccgggcatcg tggccgtccc ccctgctcag
     2881 cgcatctgcc gggtcagccc ggccgtgcgt tcggcgatca ccatcagcgt cagcgtcagc
     2941 agcacgatca ggcccgaggc cgcggcggtg cgcacgtcga ggttgttctc gatctgctgc
     3001 cacaggtgga tcggcagcac ctcggtacgc tcgtcggcga gaaagagcga caccggcacg
     3061 ttgtcgaacg aggccatgaa cgcgaggaag gctccggccc cgatgccccg ggcgatcagc
     3121 ggcagcgtca cgcgccggaa cgtcgtccat tcgctggcac cgaggctgcg cgaggcgtcg
     3181 agcagcgagg gttcgagctg cgtcagggcc gcgatcgtcg tgcgcagcac gaacggcgtg
     3241 cagacgatca cgtgcccgag caccagcagc ggcaggtccg gccgcaaccc gagccggtgg
     3301 atgaagatca gcgctgcgaa gccgaaggcg agcgccggca gcagcagcgg cgacatgaac
     3361 agcacatcgc aggcgcgcac ccacggactc ttgctgcgcg cgatcgcgat cgccgccggc
     3421 gtgccgagca gcacgcacag cacggtcgtc cagagcgcga ccacgaggct gttccaggcc
     3481 gcggcctgca tctggtcggc atcgagcagc gcggcgtacc agcgcagcga gaagccttcg
     3541 ggcggaaagc gcagcgattc ggtggccgtg aacgaggtca gcaggatcac cagcgtcggc
     3601 gcgaccagga acaccagcgc cagcgccgcg agcacgccga acacgcatct cagcgaccag
     3661 gcctcgaacg acggggcggc ccggcgcggc agcggcggcg ccggcaggac gagggtttgc
     3721 tcagacatgc ttttcgtacc tccggccgaa accgttcagc agcaccacga ccagcagcac
     3781 cgcgatcatg aacaccaccg acaccgccgc cgcgaacggc cagtcgttgg caccgaccgc
     3841 ctgctggtag atgaacagcg gcaggtacag caggcgcgcg ccgccgatca gcgtctgcgt
     3901 gacgaaggcg gtgatgctgg cggcgaagct caggatgcag ccggcgatga tgccgggcat
     3961 gaccagcggc agcgtcacgc gccagagcgt gcgccattcc ccggccccga gcgcacgcga
     4021 ggcatcggcg aggttcgggt cgaggcgcga cagcacggtc aggatcggca gcaccatcag
     4081 cggcatctgc acctgcgcga gcaccagcac gacgccggcc tcggaataca gcagcttcag
     4141 cggcgcatcg atcagcccga gcccgagcag gatctggttc agcaggccct ggcgcccgag
     4201 gatcacgatc cacgagaagg tgcgcacgac gacgctggtc aggatcggca gcaccacgag
     4261 gaagatcagc acgctctgcc agcgcgcccc ggcccgcgcg cagacccacg cgaccgggaa
     4321 gccgaacagc aggcatacga gcgtcgcctc ggcgccgagc agcagcgtcg agccgaggac
     4381 cgacaggttg aagcgatcgc cgaggaagcg cgcgtactgc gcgagcgtga ggccggcgct
     4441 gccgtcatcg ccggcagcga gcgacagcat cagcagcacg cacagcggcg cgacgaagcc
     4501 ggcggcgaac agcgccgtca gcggcagggt cagcggggcg cggggcgcgg ccatgctcag
     4561 acccggatct cgcggttgaa gcgttcgatc agcgcgctgc gctgctcggt cagcaccgcc
     4621 cagtcgacgc cacgcagcgc cgcgagcgcc tcctgcgtct tcgcgaccga ctgcgtcacg
     4681 gcgccggcga gcgccacctt gccgttggtc gggatcacgt cgtacggcgg ctgcatcatc
     4741 gccgcctgca cgagcggatc gaggtgggtg tcgatgtact gcacggccag ctccggcacc
     4801 gtcgtgccgg cgaccacgtg caggctcgtg tgccaggcga ccgcgccggt ctcgggcacg
     4861 acgaactcga ccggcacgcc cttggccttc agcgtctgca cgaagttgaa gttgtacggc
     4921 gcgatgtcga tctgctcctg ctgccacagc gtcgcgaacg cgccgaggtt cgccgccacc
     4981 gcgccgagcg cgggcagcag ctcgcgcaat gccctgaacg ccggttcgaa gtcgctgctg
     5041 ctgccaccgc gcagccggtt cagctcggcg aggaaggcca gccccagctg gctgttgagc
     5101 gccgtcatgc cgacgcggcc cctgtactgc ggatcccaga gatcgtccca gcgcttcggc
     5161 ggggttttga tccgctccgg gttgtaggca aggccgatga cctgcatcga gaccttcggc
     5221 ccccagcggt cctgatacac cggcagcagc tcggcgtagt gcggcgactg cgcggccggg
     5281 tattcggcga tcaggccctg gcgcacggca tcgagcacct gcggcggatc gaagatcgcc
     5341 acgtcgaacg gcggcgtctg gcccttcgcg gccgcgaggc gcgcgacctg gtcggtgccg
     5401 aggatgatcg actgcgtgac gcccgcatcg acgcgcttct ggaaggccgg cttcagcacg
     5461 ttgcggtgcg cctcgctcca ggtgccggga taagtcgccg ccaccagctt gccggcctcg
     5521 gcacgcgccc ggaccgccgg cagcagcgcg cccagcgcca gggcaccgcc ggccatcagc
     5581 agttcgcgtc gggtcatcag gaatcgctca ttcatcgtct gctgcctcgg tatcaggaag
     5641 ggaaagcggg cggaaaccgc ccgtcgcggc acgtcgtcag ggagggttca tgcccgcttc
     5701 cggcgcgggc gatgccgccg ggccggggga gccggaaacg gctgcggacg gcagggctgc
     5761 gccctcaggc cggaaacgcc cggtagccgg ccgatggctg cggggcggcc cagacctgcg
     5821 ccccggccgg gagcagtgcc tcgccgcgct gacgcggcac gctgaccttc agcgccgcgc
     5881 cgccctcgag cgcgaggtcg tagacgacga gcgcaccgag cggcagcgcc gtctgcaccg
     5941 cgccgggcag cgcatcggcg cccggtgcct cggcgtgcag gtgcaggtgc tcggggcgga
     6001 tgctgacgcg caccgcgctg ccggccgtca gcggcgcatc gctggcgacc cggatgcgcc
     6061 ggccgccggc cagcgccacg accgggctgc cgtcggcatc ggccgtgctg accgtgccgt
     6121 cgagcacgtt ggtcgagccg acgaactggt tcacgaacag cgtcgcgggc cggtcgtaga
     6181 tatcggtcgg cgaggcgatc tgctcgacgc ggccgcggct catgaccgcg acgcgatcgg
     6241 ccatcgacag cgcctcctcc tggtcgtggg tcacgagcac ggtggtgatg ccgtactcgc
     6301 gctgcaggcg cttgacctcg atctgcatgt cgaggcgcag attcttgtcg agcgccccga
     6361 agggctcgtc gagcagcagg atcttcggat cgacggcaag gcagcgcgcg agcgccacgc
     6421 gctgctgctg gccgcccgaa agctggcgcg ggaagcgccc ggcgaactcg ctcatgtgca
     6481 cgagcgcgag catctcgtcg acgcgcgcat cgcgcttcgc cttcgccacg ccctgcactt
     6541 cgaggccgta ggcgatgttc tggcggatgc tcaggtgcgg gaacagcgcg tagttctgga
     6601 acacgatgcc gacgccgcgc cggctcggcg acaggtgatc gaccggctcg ccgtcgaaca
     6661 gcacccggcc ctcggtctgg cgcatgaagc cggagatgat ccgcagcagc gtcgacttgc
     6721 cgcagcccga aggcccgagc agcgccacca gttcgccggc accgatgcgc aggttgatgt
     6781 cacgcaccgc cacgaagtcg tggaagcgct gggtgatggc ttcgagctgc agctcgtgtc
     6841 cgcgttgtcc gctcatggcg atcagctccc ccggtaggtg gtgtaactgg caggcgtgca
     6901 cagcagcggc acgtggtagt gcagggccgc atcggagacg acgaaacgca ccggcaccgt
     6961 gtcgaggaaa ccgccgccgt cgtgaccgcg gctgcggaag tagtcgccga tgcggaattc
     7021 gatctggtag gtgccggccc gcatcgcgtc gtcgcagagc agcagcgggc agcggccctc
     7081 ggcatcggtc tcgaagccgc cgagccggcg ccaacctccg gcgtgctgca ccgacagcgt
     7141 cagcgccacg ccggcggccg gcaggccggc gaccgcgtcg agcacatgcg tgctcagcca
     7201 gcccggcgcg atcccgaagc gctgcgccag ccgcccgcgc acgacctcgg ccacctgcgt
     7261 gagcgcgacc ccggcctcgg tggccgcggc gttcgcgagc cggcgttcgc cctcggcgaa
     7321 gatcgaggcg agatccgcgt gcaggcgcac ggccgtgatg aacggaaagc cgaaacgctc
     7381 gcggtaggcg gtgttcagcg ccctcaggcg ggcgtgctcg gccgcactga ggcggtcgag
     7441 gccgaggcgc gactgctcgc ggttcgaatc cgcggtcagc gtgccctcgc ccgcctcgcg
     7501 cccggccagt tccggatgac gctgcagcag cgcggtgcgc gtgtcggcat cgctggcgcg
     7561 cagcacgccg gcgagcgccg catgcagcgc ccacaccgac gcgaacggcc gcagcgcggc
     7621 gacgcggcgc acgacccacg gggagtgttc gacgacctcg ccgagggccg cgacgaaggc
     7681 gtcctccggg caggcattca gggcttcgag ctgggcggcc gcgtcgcggt ccggaatcgg
     7741 aatgtccacc gtgcgcctcg tctgttgcag tgcgacaggc gcaactctgg cgctcggcgg
     7801 gcattgccgt ccatctgata ataagcaagg ctgcgttcga taaattcgaa cgggaggacg
     7861 cgatgcccca gtccctgctg ccgaagatgc tgcgctatgc cgaggtcgtc gcccgtgccg
     7921 gttcggtgca ggccgccgcg cgcgtgctga acctgtcggc ctcggcgatc gaccgccaga
     7981 tcctgctgct ggaggatctg ctcggcgccc cgctgttcga gcgccagccc gacggcatgc
     8041 gcctcagtgc cgccggcgag ctggtggtga cgctggcgaa gcgctggcag cacgatgcca
     8101 gccggctgct gaccgacatc cgccagctgc agggcatcaa tcagggcagc ctgcggctcg
     8161 cggcgatgga cagccacgcc aacggcctga tgccggtgtt cctcgaacgc ctgcaccgcg
     8221 agcatccgcg catccggctg gagatcgaca tcgtcagccc cgatcaggcc gaggccgcgc
     8281 tgatcgccgg cacgaccgac ctcgcgatcg cgttcaacct gaaggccggt cgcgacctgc
     8341 acctggtcat gcaggccgaa ctgccgctcg gctgcgtcgt cgcgcccggc cacccgctcg
     8401 ccggccagcc cgacaccagc ctgaaggcgg tctccggata tccgatcgtc tcgcagaacc
     8461 gctcgctgac gatccgccgc tacctggaga gccgccacgg ctggctgttc gccgaacgcg
     8521 aggccccggt gcagaccaac tcgctgcaac tggtcaagca cctcgccgcc gccggcagcc
     8581 acgtcgcgct gacctcggaa ctcgacgccg ctcccgaaat cctcgccggg cgtctgtgct
     8641 tcatcccgct gaccgaccgc aacgtgcagc cgcagacggt cgcgatcgca atcagcgcac
     8701 gccggccctt gccgcgcatc ggacggctgg tggcggattt gctggcggag gaattgcggg
     8761 gctgtctgga ggccgtgcgg caacggcgtg cggccggcag cggcggctga gcggggccgg
     8821 cgctgatttg cggcccatga aaaagccgcc ggtcccctgc ggggaaccgg cggctcttgc
     8881 cagtcaatgg cggagaagga gggattcgaa ccctcgatac gcggttagcg tatacacgat
     8941 ttccagtcgt gcgccttcga ccactcggcc acttctccgg aattccgtac tcgccgcaag
     9001 gtggcggcga gcgcgcgaga gtacggcatc atccgcaccg atcgcaagcg attcagcgac
     9061 gatcggtaca gatgtccgac cgtctcaggc gtcgacctga cgcatggtca ggcgcacgcg
     9121 gccctgcttg tcgatttcca tgaccttgac gcgcacgctg tcaccgacgg cgagcttgtc
     9181 ggtgacgctc tggatgcgct cctcgcagat ctgcgagatg tgcaccatgc cgtcacgccc
     9241 gggcagcagc gacacgaagg cgccgaagtc catgatgcgc acgaccttgc cttcgtagat
     9301 gcgaccgacc tcgatgtcgg cggtgatcat ctcgatgcgc cggcgcgcct cgtcgctggc
     9361 gctcttgtcg accgcggcga tcttgaccgt gccgtcgtcg tcgatgtcga tcgtcgtgcc
     9421 ggtttcctcg gtgagctggc ggatgaccgc gccgcccttg ccgatcacgt cacggatctt
     9481 gtcgggattg atcttgatcg tgacgatgcg cggtgcgaac gaggagatgt cgtcgcgcgg
     9541 cctgtcgagc acggcgttca tgcagccgag gatgtgcagg cggccttcgc gggcctgggc
     9601 cagcgcgacc tgcatgatct ccttggtgat gccgtcgatc ttgatgtcca tctgcagcgc
     9661 ggtgacgccc tgcgtggtgc cggcaacctt gaagtccatg tcgccgaggt ggtcttcgtc
     9721 accgaggatg tcggagagca ccgcgaagcg ctcgccttcc ttgatcagac ccatcgcgat
     9781 gccggccacc ggtgccttca gcggcacgcc ggcgtccatc agcgccaggc tggagccgca
     9841 gaccgaggcc atcgagctgg agccgttcga ctcggtgatc tcggagacca cgcgcacgac
     9901 gtacgggaac ttctccatgt ccggcatcac ggccgcgacg ccgcgcttgg ccagacgccc
     9961 gtggccgatc tcgcggcgct tcgggctgcc gacgcggccg gtctcgccga ccgagaacgg
    10021 cgggaagttg taatgcagca tgaagagctc gcggtactcg ccttcgagcg cgtcgatgat
    10081 ctgcgagtcg cgggtggtgc cgagcgtggc gacgaccagc gcctgcgtct cgccgcgcgt
    10141 gaacagcgcc gagccgtgcg tgcgcggcag cacgccggcg cgcacggtga tcgggcggac
    10201 cgtcttggtg tcgcggccgt cgatgcgcgg attgccgtcg aggatgcggt cgcgcacgac
    10261 gcggtattcg aggttcgaat aagccgtctt gacggcttcc ggcgtgtagg cggcaccttc
    10321 accggcgagc tgctcgacca ccgagccgcg gatttcgcgc acgcggttct ggcggtcctg
    10381 cttgacggcg atctggtagg cctcgaccag cgatccgaag gcggcgctgc gcacggcggc
    10441 atcgagcgcg gtatcggcgg ccggggccga ccagtcccag gccggcttgc cggcttcggc
    10501 ggcgagttcg cggatcgcgc ggatcgcggc ctgcatctgc tcgtggccga acacgacggc
    10561 gccgagcatg acttcctcgg agagctcctg cgcttcggac tcgaccatca gcacggcgtt
    10621 ttcggtgccg gcgacgacga gatcgagctg cgaggtcttc agctccgagt gcgaggggtt
    10681 cagcaggtac tggccgttga tgtaaccgac gcgcgcggca ccgatcgggc cgccgaaggg
    10741 catgccggtc agcgcgaccg cagccgaggc accgattagc gccgggatgt ccggatcgat
    10801 gtcgttgttg gccgacatca ccgtcgcgat gatctggact tcgttgtaga agccttcggg
    10861 aaacagcgga cgcagcggcc ggtcgatcag gcgcgaggtc agcgtttcct tttcggacgg
    10921 gcggccttcg cggcggaaga agccgccggg aatgcggccc gcggcatagg tgcgctcctg
    10981 atagtcgacc gtcagcggga agaagtcccg ccccggcgcg gcttccttcg cccccaccgc
    11041 ggtgaccagc accaccgtgt cagccatgct gaccatgacc gcgccgtcgg cctgacgggc
    11101 gatttcgccg gtttccagcg tgacggtgtg ctgaccgtac tgaaaggatt ttttcacgga
    11161 agccacggga ccatcctgaa gtgctgcctg ggaagcggta atgcgaggcg gcgtgcgtac
    11221 gacggaatca gcgacgcagg cccaggcggg cgatcagatc ctgataacgg ccgaggtcac
    11281 cgcgcttgag gtaatcgagc agcttgcggc gctggttgac catcttcagc aggccctggc
    11341 gcgagtggtg atcctgcttg ttcgactgga aatgcggctg caggccattg atgcgcgcgg
    11401 tcagcagcgc gacctggact tccggcgagc cggtgtcatt ggcggcacgc tggaattcct
    11461 ggacgacctg ggccttctgt tcgttgctga gggacattga ggcactccga attctgattc
    11521 cgtgtgcgga atcgggtggg tataaaagaa cgcgcgattc taaccgtccc caggctgccc
    11581 cacaaggcaa gcgcagggga cgccatgcaa gtctgcggcc ggatgaagca cgtcgtgccg
    11641 cctgtcctgc ggatgcggcc cggcagcgtt ttcaggacgc attcagcacg cgccgcggcg
    11701 cgacgcggcc atcgccggtc gggcagccga tgccgagcag gcggccgttg ccgtagaccc
    11761 gcacctcacc gtccaccggc agcgggttcg cccgcaccgg gttgccgttg acgaagccgt
    11821 gcgcggcttc gtcgccgagt tcgaccctcg gccagccggc gagcgcgctg tcgatcggca
    11881 gcagacaggc atcgagggcg tcgcggtcct cgccgagggc gcgcagctga tcgagcgtca
    11941 gcatgcgcgc ggcatcccag ggctcgacgc cgatgcggcg cagttcgagc aggtgcgccc
    12001 cgcagccgag tgccgcgccg atgtcctcgg cgagcacgcg gatgtaggtg cccttcgagc
    12061 aggcgacctc ggccgagaag ctcccggcgc tccagtcgag cagctgcagg ctgtggatcg
    12121 tgacgctgcg cggggcgcgc tcgacctcga tgccctggcg ggcgagctcg tacagcggcc
    12181 ggccgtcgcg cttcagcgcc gaatgcatcg gcggcacctg ttcgatcggg ccgcgaaagc
    12241 gcgtgaacac ggtttcgagc acgccggcat cgatcggcgg cagcggccgc tcggcgagca
    12301 gcgcgccttc ggcatcgccg gtcgtggtgg tgacgccgag tttcgccgtg aagcgatagc
    12361 gcttgccggc gtcgagcagc aggcccgaga ccttggtcgc ctcgccgaac aggcagggca
    12421 gcaggccggt ggcaagcgga tcgaggctgc cgccgtgccc ggccttgcgg gccgcgtaca
    12481 gccatttcac tgcctgcagc gcggcgttcg aggacaggcc atggggcttg tcgagcagca
    12541 ggatgccgtg cacgtcgcgc cgttcccggc gcacgcgcgg ctgggtttca cgggaactca
    12601 tgccggatcg tcgtcgccgg gatgatcgtc cgcgtcgtcg gggtggcggg ccgcgtcttc
    12661 ggcgaccgcc tgacggatca gcgcatcgag cttcgcgcca cgctcgatcg attcgtcgta
    12721 gacgaaggcg agccgcggca cggtgcgcac gtgcaggccg cgcccgagct gctggcgcag
    12781 cagaccggcg tgctcggcga gctgcttcag cacggccgga cgctcggcct cggggccgag
    12841 gtaggtgacc cagaccttgg cgtgcgcgag gtcgcggctg atctcgacct gcgtgatcga
    12901 gaccattgcc acgcgccggt cgtcgatctc gctgcgcatc agcgcggcga gatcacggcg
    12961 cacctgttcg ccgagccggc gggtacgcga gaactccttg gccatcggct gcgggctccg
    13021 ttgcgcttac agcgtgcgct ggacttcgat gcgctcgaag acctcgatct ggtcgccttc
    13081 gcgcacgtcg ttgtagttgc gcacgccgat gccgcactcg aagccctgcc ggacctcggc
    13141 gacgtcatcc ttgaagcggc gcagcgagtc gagctcgccc tcgaagatga cgacgttctc
    13201 gcgcagcacg cggatcggcg ccgagcgctt gaccacgccg tcgacgacca tgcagccggc
    13261 gatcgcgccg aacttcggat ggcggaacac ctgacgcacc tgcacgaggc cgatgatctg
    13321 ctccttgacc tcgggcgaga gcatgccgac cagcgccttc ttgatctcgt cgatgacgtc
    13381 gtagatgacg ctgtagtagt gcaggtcgac gccttcctcc tcgatgacct tcttcgccgc
    13441 gctgtcggca cggacgttga agccgatcat caccgcctcg gaggcgacgg cgaggttggc
    13501 atccgattcg ttgatcgcgc cgacgccgga cgaaatgatg cgcacgcgca cctcgtcggt
    13561 cgacagcttg gtcagcgcct cggacagcgc ctcggccgag ccctgcacgt cggtcttcag
    13621 cacgacgttg agcgtcgcga tctcgccctc ggccatctgg ctgaagacgt tctcgagctt
    13681 ggccttctgc tggcgcgcca gcttgacctc gcggaacttg ccctggcgga acagcgcgat
    13741 ctcgcgcgcc ttgcgctcgt cgctgaccac gaccgcctcg tcaccggcgt tcggggcgcc
    13801 ggagaggccg agcacctcga tcggaatcga cggaccggcc tcgtcgatcg gccggccggt
    13861 ctcgtcgagc atcgcgcgca cgcggccgta ttcgagaccg gtcaggatga tgtcgccctt
    13921 gcgcagcgtg ccggactgca ccagcagcgt cgcgaccgga ccgcggccct tgtcgaggcg
    13981 cgactcgatg accacgccct tggccgggcc gtcgacggcg gcggtcagct ccagcacctc
    14041 ggcctgcacg aggatctggt cgagcaggcc gtcgatgccc tgtccggact tcgccgacac
    14101 gtaggcgaag aggttctcgc caccccactc ttccgagatc acgccgtgct gcgacagctc
    14161 gctcttgacc cgttcgagat cgatgccgtt cttgtccatc ttgttgacgg ccacgacgat
    14221 cggcacgccg gcagcgcgcg cgtgctggat cgcctcgacc gtctgcggca tgacgccgtc
    14281 gtcggccgcg accacgagca cgacgatgtc ggtcgccttc gcaccgcggg cacgcatcgc
    14341 cgtgaacgcc gcgtggccgg gggtatcgag gaaggtgatc gtgcccttgc cggtctcgac
    14401 gtggtaggcg ccgatgtgct gcgtgatgcc gccggcctcg ccggccgcga ccttggtgcg
    14461 gcggatgtag tcgagcagcg aggtcttgcc gtggtcgacg tggcccatga tcgtgaccac
    14521 cggcgcacgc gagcgcgcct cgtgcacgac gcccatctcg gcggccagtt cgtcctcgag
    14581 cgcgttctcg gacagcagct tgtagctgtg acccatctcc tcgacgacga tcgcggcggt
    14641 ttcctggtcg agcacctggt tgatcgtgac catggtgccg agcttcatca gggtcttgat
    14701 gacctcggtg gccttgaccg ccatcttcgc cgccagctcg gcgaccgtga tggtctcggg
    14761 cagcgccacc tcgcgcgtca tcggcgacac cggcttcacg aagccctgct tggacgccgc
    14821 gccgaccagg cgaccgccct tgccgccctt cctgcccttg ccacggcgat cgccgtcgaa
    14881 gtcgccgccc ttgccacgcg gttcgtcgtc acgacgccgg ccgcggtcat cgctgccacg
    14941 gcggcgcgtg ttgtcggccg tcgccggtgc cgccggtgcg gcggcaggcg cggcatccgc
    15001 gccggcgggg cgacgcggcc ggtcctcg
//
LOCUS       DAOQIE010000049        14878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1918_length_14878_cov_6.554004, whole genome shotgun sequence.
ACCESSION   DAOQIE010000049 DAOQIE010000000
VERSION     DAOQIE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14878
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_14878_cov_6.554004"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1091)
                     /locus_tag="PK144_04830"
     CDS             complement(153..1091)
                     /locus_tag="PK144_04830"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF65086.1"
                     /translation="MEQRLKQRLLGTAVLVALAVIIVPEVVKTSGPVTSPAVATSIPP
                     RPESPQPGGVTVALPPAILPQAVPPEAPAPSGQAPASTIAQSPVIEPLRTPAALPSAS
                     AGGDGPQGMRTVDLGAGAPRVAPANPAPAAPPARPAAPKPAPVVASAPAPKPAQTPAA
                     VATPSKPAPETARATTDAPKPKPEVVAKAAPAAVTPAATARPVSLPKIELTSRDTSRA
                     SASAPAAEPWRPQASNGSWAVQVGSFGVEENANLLRDRLRSQRFPAVVERIRGGSGQA
                     MYRVRVVGQDSRPASEAIASRLRSAGVAANIVEPGE"
     assembly_gap    1163..1262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1277..2542)
                     /gene="folC"
                     /locus_tag="PK144_04835"
     CDS             complement(1277..2542)
                     /gene="folC"
                     /locus_tag="PK144_04835"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011409300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNF65087.1"
                     /translation="MPAALRSDWSLADWLAWQETLHPKGIDLGLERVRAVAARLDLLQ
                     PSARTIVVGGTNGKGSTLAFLEAMLLAAGRRVAAYTSPHLLRYEERLRLDGRECPAGD
                     WCRAFAAVDAARGDQSLTYFEFGTLAALWLARAFDPEVLVLEVGLGGRLDAVNIIDAD
                     VAIVTTIGIDHTEFLGPDRESIGCEKAGIYRPGRPAICVDPSPPASLLAHQQAIGARP
                     YRFGHEFGVREARDGWGWWGPGGGLDLPWPSLAGPHQLANAAGAVAALRLLPSIEVSG
                     AAIASGIAAARLAGRFQIESGPVEWVFDVTHNPHGAAVLAETLQWRPVTGQTWLLLAM
                     LADKDAAGVITALMPVVDRWCAAGLDGARGRPVESMLASLPAGTMACKDVTAACRQLE
                     SAAQAGDRVVVCGSFLTVAEAMRARHITC"
     gene            complement(2547..3431)
                     /gene="accD"
                     /locus_tag="PK144_04840"
     CDS             complement(2547..3431)
                     /gene="accD"
                     /locus_tag="PK144_04840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNF65088.1"
                     /translation="MSWFEKLMPSRIRTEGGNKRQIPEGLWSKCDACSAVLYRAELER
                     NLHVCPKCDHHMRIRARERLASFLDADGRVEIGAEVEPTDFLKFKDSKKYKDRLAAAQ
                     KSTGEKDALVAMSGTVLGVPVVASAFDFEFLGGSMGSVVGERFVRAARVALETRVPYV
                     CFTASGGARMQEALVSLMQMAKTSAILARLAEAGVPFVSVLTDPTTGGVSASLAMLGD
                     LNVAEPKALIGFAGPRVIEQTVREKLPEGFQRSEFLLEHGAVDLIIDRRELRARLGAI
                     LAMLSGRPQPLAQAEAAV"
     gene            complement(3509..4321)
                     /gene="trpA"
                     /locus_tag="PK144_04845"
     CDS             complement(3509..4321)
                     /gene="trpA"
                     /locus_tag="PK144_04845"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNF65089.1"
                     /translation="MNRIQTRFDALRTAGRRALIPYITAGDPDLGTTETLLHTLVASG
                     ADIIELGVPFSDPMADGPVIQRACERALAAGTRLRDVLALVRRFRERDADTPVVLMGY
                     LNPVEAMGYAAFAEAAAQAGVDGVLTVDLPPEEAHELTQALLASDLDPIFLLAPTSTE
                     ARIRRIGALARGYLYYVSLKGVTGSARLNTGEVAEKVARIKALVDVPVGVGFGIRDAD
                     SAAAVARVADAVVVGSALVSRIEALAGDPQQLNAEIGALLGAMRTAMDAVTA"
     gene            complement(4335..5546)
                     /gene="trpB"
                     /locus_tag="PK144_04850"
     CDS             complement(4335..5546)
                     /gene="trpB"
                     /locus_tag="PK144_04850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNF65090.1"
                     /translation="MGDVIDFSRFPDADGHFGPYGGLFVAETLMAALEELRDAYARFR
                     DDPDFLAELDRDLSHYVGRPSPLYHAERLSRNWGGAQILLKREDLNHTGAHKVNNTIG
                     QALLAKRLGKTRIIAETGAGQHGVATATIAARLGLECVVYMGAEDVKRQSPNVYRMKL
                     LGASVVPVQSGSKTLKDALNEALRDWVTNVDDTFYVIGTVAGPHPYPMMVRDFQCIIG
                     REAREQVLAGFGRLPDALVACVGGGSNAIGLFHPFLNDASVAMYGVEAAGDGLDTGRH
                     SAPLNAGRPGVLHGNRTYLMEDENGQIIETHSVSAGLDYPGVGPEHAWLKDIGRVRYG
                     AITDDEALAAFHELCRTEGIIPALESSHALAYAKKLAPTLAADQLLVVNLSGRGDKDI
                     FTVAARDGITI"
     gene            complement(5539..6171)
                     /locus_tag="PK144_04855"
     CDS             complement(5539..6171)
                     /locus_tag="PK144_04855"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNF65091.1"
                     /translation="MHRTRVKICGLTRAEDALACVRAGADAIGLVFVPKSPRCVSIEQ
                     AAAVIAVLPPFVACVALFQNAGAEYVRAVMAHLSPTLLQFHGDEPREFCSGFGRPYLR
                     AVPMGAGAEVDDYARRFPDAAGLLLDSHGGERSGGSGEAFDWSRIPARRTLPLVLAGG
                     LDPDNVAHAIYRVRPWAVDVSSGVEAAKGIKDARKIGAFMQAVRNGDDRG"
     gene            complement(6269..>6731)
                     /locus_tag="PK144_04860"
     CDS             complement(6269..>6731)
                     /locus_tag="PK144_04860"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HNF65092.1"
                     /translation="YRYVILNRRYRPALTAGRATHWYRPLDAAHMHEAGQALLGEHDF
                     SAFRAAGCQAEHPRRALYALEVARHGDLVVLEVEANAFLHHMVRNIAGVLLAIGGGER
                     PIAWAAEVLAGRDRRHGGVTAPADGLYFLHVQYPQRFGLPSGNPSALPVLF"
     assembly_gap    6732..6741
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6742..7057)
                     /locus_tag="PK144_04865"
     CDS             complement(<6742..7057)
                     /locus_tag="PK144_04865"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HNF65093.1"
                     /translation="MRIALGIEYDGSAFRGWQTQEEGVRTVQGALEAALTRIADRPVA
                     VTCAGRTDAGVHGAGQVVHFDTDAVRSAHAWVMGSNSQLPPDVAVTWAQPVPEDFHAR
                     FSA"
     gene            complement(7114..7782)
                     /locus_tag="PK144_04870"
     CDS             complement(7114..7782)
                     /locus_tag="PK144_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65094.1"
                     /translation="MNPVILLASFVAFALGIAPAAPPALLLGALLLAFARWGTGPVLP
                     AGTLRRALRRVRWLLLAVVITHGWFTPGLTLLPALGGASPSVQGLWLALWRALVLLEL
                     VAAAALVLALAGTHGVAGALYRLAPAGGGFRERLAVRVALCFEALPLLQARMTAPADA
                     GSGRLGRWTGAARAVFAAAGQPAADFAPADRLTVAVPPLPALREWRLPLAIALAFAGM
                     HALA"
     gene            7959..8492
                     /gene="moaB"
                     /locus_tag="PK144_04875"
     CDS             7959..8492
                     /gene="moaB"
                     /locus_tag="PK144_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNF65095.1"
                     /translation="MPESSCRTDRLRPLAIAVLTVSDTRTAAGDQSGALLVERLQDAG
                     HQLVARALEPDDLYRLRARVSQWIADAAVEVVLVTGGTGPTGRDRTPEALRPLLDKEL
                     EGFGELFRQLSYQEIGTAAAFSRALAGVANGTLVVALPGSPGACRTAWDGILAGQLDS
                     RRGHCNYVALMPRLTET"
     gene            complement(8523..8858)
                     /locus_tag="PK144_04880"
     CDS             complement(8523..8858)
                     /locus_tag="PK144_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65096.1"
                     /translation="MSASVALPVADHRLAVLLRRYEDILANSCPAFSDAEWSALSEAL
                     ADINLEETDALRRLYATVCHEIQRRGLDGKWHIDARALMHRLGGLSRAEEAALVEIVE
                     FRLGRGRGI"
     gene            complement(9054..10391)
                     /locus_tag="PK144_04885"
     CDS             complement(9054..10391)
                     /locus_tag="PK144_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNF65097.1"
                     /translation="MNAGLSYAASPPLALPLRWFLSVPVFGVLAGGWLLWAGPAVMAG
                     RWQPQALAYVHLLALGVLLSAMLGALIQLLPVLVGAPLRRAAPIAAGVQALLLPGIVL
                     FAGGLGTGAAPLVRFGSALLLCALLVYAAAALPAWWRSPRSGESARTIGWAGLSFLIA
                     AALGFTLAAGHGWAGVPLLRRLTDLHAGWALIGWVGLLVAAVAWQVVPMFSITAPYPR
                     WLRTGLAPVSVAALGLASLDAGWPLPDWLCHAAALAAGAALLGFAACTLWLQHRRRRR
                     LPDPWLGYWKLALGCAALAVCLWLAGRFGLRTGSGGSGSSTEMLSGLLWLLGFGTTLV
                     AGMLQRIVAFLVWLDLSTRLRAVRSAALAPNVRELIPAAALQRQFAAQFLSVGLTLAA
                     CTLPALARPAGAAGVLAWGLLALNLVRAIRTGRGWRQRIGDGRAAVAEQSVTI"
     gene            complement(10388..10669)
                     /locus_tag="PK144_04890"
     CDS             complement(10388..10669)
                     /locus_tag="PK144_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNF65098.1"
                     /translation="MTTAEHLLDVGGLEPPEPLFATLDAVDALGPGEWLRLLHRREPF
                     PLYGHLLERDCAYRVLVSEPGRYEALIWRLDDPLASAACAGDDDGDGCA"
     gene            complement(10691..11137)
                     /locus_tag="PK144_04895"
     CDS             complement(10691..11137)
                     /locus_tag="PK144_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNF65099.1"
                     /translation="MNPLNELLEGQHRQADAALADAELQVAAGDWPAARAAGSALDDE
                     LRAHFRREEAVLFPAFEAATGSGCGPTRVMRDEHEQIRTLLDALRGAGERCDADAWLG
                     LSDTLVLLMQQHNMKEEQILFPMAARLLAARLPALLDGLRPAAVSA"
     gene            11289..11984
                     /locus_tag="PK144_04900"
     CDS             11289..11984
                     /locus_tag="PK144_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF65100.1"
                     /translation="MPPSPAPEALDRLLRQTYLFAALDEPRLAVVRHGMRVLHLHEGQ
                     RLFDVGQPAERFFLVASGQIKLFRVSLDGNEKVIEIAGPGKTFAEAVMFMQRRDYPVS
                     TAALTEARVCSFDNHTYLELLRASPSLCLSMLGEMSMRLHARLNEIDHLTLQNASFRV
                     VHYLIDQLIEHGDGSGSIDLSTPKNIIASRVSIKPETFSRILRTLADEGIVRIDGKHI
                     EVPDCRRLQQWGR"
     gene            12143..13978
                     /locus_tag="PK144_04905"
     CDS             12143..13978
                     /locus_tag="PK144_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF65101.1"
                     /translation="MVPSLRQRSPSTADDGLATTVSNHIGLAYFEALVRRLAVALDAG
                     FACVARHEPETGRLLPVASHSTDDAHDWPAALDGAPWPLVLAGVTVLQADGADALYAG
                     NTALAGVRACAGTPLVDAGGRTLGLIAVLGRRPFAQPAMVALVLELFAERAAAELERL
                     AAERELQRTGAALRRQQLALLRIVHAELLVEAEADTALARIAEIAGETLDADRAEIWQ
                     ADAAFGQVHRCASWHHRHGRGHDHDGPALDTRDCPGFVGTLRAQMLLDGSDPRHAQAL
                     AEWRALAPPDSEAGASVLVLGISSGRTLHGLLQLECRRTAAARVWGIDELGFARSVCD
                     LLALTLVTRALRRTVAELEASQQRYRALYEDIDTLARERLQALETHQQRMHQMQRQFV
                     YMASHELRTPLAIIDSAAQRILRRADNLSRTELVERIGRIRAAVSRMGYLVDSTLDVA
                     RFESGTVSYRPGPVDPAALLTRVCQQQQELAPHHRIACRFEALPPSMSGDAGLLEQVF
                     ANLLSNAVKYSPDADEIEVHAFADATRLVVRVCDHGVGIPAQEMPRLFEKFFRASTAV
                     GIPGTGIGLNITRQIVDLHGGILTVDSHVGTGTTFTVKLPLPSAA"
     gene            14672..>14878
                     /locus_tag="PK144_04910"
     CDS             14672..>14878
                     /locus_tag="PK144_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65102.1"
                     /translation="MIVPYVRDRLPAPPAASSASVQSFSAPPLAQRNLPAPAAPVPPQ
                     ATPAPSYPPDPVQARARQTLATLAD"
BASE COUNT         2311 a         5294 c         5151 g         2012 t
ORIGIN      
        1 aggcgcgcat tctacacgcc ttcttcaatt ccgcaagcag tgttttcacc gcttttcatc
       61 gacatcaaac aagtcctttg atgcgacgag acggacgatt atatgggatc acagaagctt
      121 gtcaagagcc gaacatgcac tgaaccccga tctcactctc ccggctcgac gatgttggct
      181 gccacgcctg ccgagcgcag acgcgacgcg atcgcctcgc tggcgggtct ggaatcctgt
      241 ccgacgacgc ggacccgata cattgcctgg ccgctgcctc cacggatgcg ttcgacgacg
      301 gccgggaagc gctgggagcg caggcggtcc cgcagcagat tggcgttttc ctcgactccg
      361 aagctgccga cctgcaccgc ccaggagcca ttgctggcct gaggcctcca gggttcggcc
      421 gccggtgcgg aagcactggc tcttgaagtg tcgcggctgg tcagctcgat cttcggcagc
      481 gaaaccggtc tggctgtcgc cgccggggtc acggcagcag gagctgcctt ggccacgacc
      541 tcaggcttgg gcttcggcgc atcggtcgtt gctctggccg tttccggagc cggcttcgaa
      601 ggagtggcca cggcagcagg agtctgagcc ggttttggag cgggtgcgct tgcaacgacc
      661 ggtgccggct tcggggctgc aggtctggct ggcggtgccg ccggtgccgg gttggccgga
      721 gccacgcgcg gcgcgccagc gcccagatcg accgtccgca tgccttgcgg accatcaccg
      781 cccgcgcttg ccgatggcag ggctgcgggc gttcgcagcg gttcaataac cggagactgg
      841 gcgatcgtcg atgccggtgc ctgcccggac ggagccggtg cttcgggcgg cacggcctgc
      901 ggcaggatcg ccggcggcag cgcgacggtg accccgccgg gctgcggcga ttccggtctg
      961 ggcgggatgc tggttgcaac ggccggcgag gtgaccggac cggaagtctt gaccacttcc
     1021 gggacgatga tcacggccag tgcgaccagt acggcggtgc cgagcagacg ctgcttcaga
     1081 cgctgttcca cgacacgact ccgggcatga actttggctg ggggcgatac gagtcagggt
     1141 ttcaggacat tcaagacatt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nngacattca agacattcag cacgtaatgt gacgcgcccg catggcttcg gcaacggtca
     1321 ggaacgatcc gcagacgacc acacgatcac cggcctgcgc cgccgattcc agttgccggc
     1381 aggcagccgt cacatccttg caggccatgg tacctgctgg cagcgaggcc agcatcgatt
     1441 cgaccggacg cccgcgtgca ccgtcgagcc cggccgcaca ccagcggtcc acgaccggca
     1501 tcagcgctgt gatgacgccg gcagcgtcct tgtcggcaag catggccagc agcagccagg
     1561 tctgaccggt cacgggacgc cactgcagcg tttcggccag cactgcggca ccgtgcggat
     1621 tatgggtgac atcgaacacc cactccacgg gtcccgattc aatctggaac cgtccggcga
     1681 gtcgggcagc ggcaatcccg ctggcgatgg cagccccgga aacctcgatc gacggcagca
     1741 gacgcagggc cgcaaccgcc cccgccgcgt tcgccagttg atgcggtcct gcaagagagg
     1801 gccagggcag gtcaagcccg ccgccgggcc cccaccagcc ccagccgtcg cgcgcctccc
     1861 ggaccccgaa ctcgtggccg aagcggtacg gccgggcgcc gatcgcctgc tgatgcgcga
     1921 gcaggctggc cggtggcgac ggatcgacgc agatcgccgg tcgtccggga cggtagatgc
     1981 cggccttctc gcagccgatc gattcgcggt cggggccgag gaattcggtg tggtcgatgc
     2041 cgatcgtggt gacgatggcc acgtcggcgt cgatgatgtt gacggcatcg agccgtccgc
     2101 cgagcccgac ctcgagcacg agcacttcgg gatcgaaagc ccgcgccagc cagagcgccg
     2161 ccagcgtgcc gaattcgaag taggtcagcg actggtcacc gcgggccgcg tcgaccgccg
     2221 cgaacgcccg gcaccagtcg ccggccggac attcgcggcc atcgaggcgc aggcgctcct
     2281 cgtagcgcag cagatgcggc gaggtgtaag ccgcgacgcg ccggccggcg gcgagcagca
     2341 tcgcttcgag aaaggcaagc gtcgaaccct tgccgttggt gccgccgacc acgatcgtgc
     2401 gcgccgaggg ctgcagcagg tcgagccggg cggccaccgc gcgcacgcgc tccagcccga
     2461 gatcgatgcc cttcggatgc agggtttcct gccaggccag ccagtcggcg agcgaccagt
     2521 cgctgcgaag cgccgcaggc atgtgctcag accgcggctt cggcctgggc gagcggctgc
     2581 ggacgccccg agagcatcgc gaggatcgcg ccgaggcgcg cgcgcagttc gcgccggtcg
     2641 atgatcaggt cgaccgcgcc gtgttcgagc aggaattcgc tgcgctggaa gccttcgggc
     2701 agcttctcgc gtacggtctg ctcgatgacg cgcgggccgg cgaagccgat cagtgccttc
     2761 ggctcggcga cattcagatc cccgagcatc gccagactcg ccgaaacgcc gccggtggtc
     2821 ggatcggtca gcaccgacac gaacggcacg ccggcctcgg cgagccgcgc caggatggcg
     2881 ctggtcttgg ccatctgcat cagcgacacc agcgcctcct gcatgcgcgc accgcccgag
     2941 gccgtgaagc acacataggg gacccgcgtt tccagcgcca cgcgcgcggc gcgcacgaag
     3001 cgctcgccga ccaccgagcc catcgagccg ccgaggaact cgaaatcgaa ggccgaggcc
     3061 acgaccggca cgccgagcac cgtgccgctc atggcgacca gcgcatcctt ctcgccggtg
     3121 gatttctgcg cggcggccag gcggtccttg tacttcttgc tgtccttgaa cttcaggaag
     3181 tcggtcggct cgacctcggc gccgatctcg acgcggccgt ccgcatcgag gaaactcgcc
     3241 agccgctcgc gcgcgcggat gcgcatgtga tggtcgcact tcgggcagac gtgcaggttg
     3301 cgctccagct cggcgcggta cagcacggcg ctgcaggcat cgcacttcga ccagagccct
     3361 tcgggaatct ggcgcttgtt gccgccctcg gtgcggatgc gcgagggcat gagtttttcg
     3421 aaccagctca tgtcgaccat ggcttgcaca acactcccgc cagcaaagcg gccggcgtca
     3481 ggaatacgga aaaggatcgc gggccggctc aggccgtgac ggcatccatc gcggtgcgca
     3541 tcgcgccgag cagcgcgccg atctcggcgt tcagctgctg cggatcgccg gccagcgcct
     3601 cgatgcgcga gaccagcgcg ctgccgacga cgacggcatc ggcgacgcgc gcgaccgcgg
     3661 cggcgctgtc agcatcgcgg atgccgaagc cgacgccgac cggcacatcg accagcgcct
     3721 tgatgcgtgc gaccttttcg gcaacctcgc cggtattgag ccgggccgag ccggtcacgc
     3781 ccttcagcga cacgtagtac aggtagccgc gcgccagcgc gccgatgcgg cggatgcgcg
     3841 cctcggtgct ggtcggcgcg agcaggaaga tcggatcgag gtcgctggcg agcagcgcct
     3901 gcgtcagctc gtgggcttcc tcgggcggca ggtcgacggt cagcacgccg tcgacgccgg
     3961 cctgcgcggc ggcctcggcg aaggccgcgt agcccatcgc ctcgaccggg ttcaggtagc
     4021 ccatcagcac gaccggcgtg tcggcatccc gctcgcggaa gcgccggacc agcgccagca
     4081 cgtcgcgaag gcgcgtgccg gcggcgagtg cgcgctcgca ggcacgctgg atcaccgggc
     4141 cgtcggccat cgggtccgag aacggcacgc cgagttcgat gatgtcggca ccgctggcga
     4201 ccagcgtgtg cagcagggtt tcggtggtgc cgagatccgg atcgccggcg gtgatgtacg
     4261 ggatcagcgc gcggcgaccg gccgtgcgca gggcgtcgaa tcgggtctgg atgcggttca
     4321 tggcggcccg gctctcagat cgtgatgccg tcgcgcgcgg cgacggtgaa gatgtccttg
     4381 tcgccgcgac cggacaggtt gacgacgagc agctggtcgg cggccagcgt cggggccagc
     4441 ttcttcgcgt aggcgagtgc gtgcgagctt tcgagcgccg ggatgatgcc ctcggtgcgg
     4501 cagagctcgt ggaaggcggc gagcgcctcg tcgtcggtga tcgccccgta gcgcacgcgg
     4561 ccgatgtcct tcagccaggc gtgctcggga ccgacgccgg gatagtcgag accggccgag
     4621 accgagtgcg tctcgatgat ctggccgttc tcgtcttcca tcaggtaggt gcggttgccg
     4681 tgcagcacgc cggggcgacc ggcgttcagc ggggccgaat ggcgaccggt gtcgaggcca
     4741 tcgccggccg cctcgacgcc gtacatcgcg accgaggcgt cgttgaggaa gggatggaac
     4801 aggccgatgg cgttcgagcc gccgccgacg caggccacca gcgcatcggg cagccggccg
     4861 aaaccggcga gcacctgttc gcgcgcctcg cggccgatga tgcactggaa gtcgcgcacc
     4921 atcatcggat acgggtgcgg accggcgacg gtgccgatca cgtagaaggt gtcatcgacg
     4981 ttggtgaccc agtcgcgcag cgcctcgttc agcgcatcct tcagcgtctt cgagccggac
     5041 tgcaccggca cgacactcgc accgagcagc ttcatgcgat agacgttcgg cgactggcgc
     5101 ttcacgtcct cggcgcccat gtagaccacg cattcgagtc cgagacgcgc ggcgatggtc
     5161 gcggtcgcga cgccgtgctg gccggcaccg gtctcggcga tgatgcgcgt cttgccgagg
     5221 cgcttcgcga gcagggcctg gccgatggtg ttgttgacct tgtgcgcgcc ggtgtggttg
     5281 aggtcctcgc gcttcagcag gatctgcgcc ccgccccagt tccgcgacag gcgctcggcg
     5341 tgatagagcg gcgacggccg cccgacgtag tgcgacaggt cgcggtcgag ttcggcgaga
     5401 aaatcgggat cgtcgcgaaa gcgcgcgtag gcatcgcgca gttcctcgag cgccgccatc
     5461 agcgtctcgg ccacgaacag gccgccgtac ggcccgaaat ggccgtccgc gtccgggaag
     5521 cggctgaagt cgatcacatc acccacggtc atctccgttt ctgactgcct gcatgaacgc
     5581 gccgatcttg cgcgcgtcct tgatgccttt tgctgcctcg acgccgctcg acacgtcgac
     5641 ggcccagggc cgcacgcggt agatcgcgtg cgcaacgttg tcgggatcga gcccgccggc
     5701 gagcacgagc ggcagcgtgc gccgcgccgg aatccgcgac cagtcgaagg cttcgccgct
     5761 gccgccgctg cgctcgccgc cgtggctgtc gagcagaagc ccggcggcgt ccgggaagcg
     5821 gcgcgcgtag tcatcgacct cggccccggc gcccatcggc acggcgcgca gatacggccg
     5881 gccgaagccg ctgcagaact cgcgcggctc gtcgccgtga aactgcagca gcgtcggcga
     5941 caggtgcgcc atcaccgccc gcacgtactc ggccccggcg ttctggaaca gcgcgacgca
     6001 ggcgacaaac ggcggcagca cggcgatcac cgccgccgcc tgctcgatcg acacgcagcg
     6061 cggactcttc ggcacgaaca ccagcccgat cgcatcggct ccggcgcgca cgcaggcgag
     6121 cgcatcctcg gcgcgcgtca aaccgcagat cttgactcgg gtgcgatgca tgggcgcggg
     6181 cggtccgggc ggcgacaagg cgcggaaggg taacaaaaac gtttcgccgc ggcacggtcg
     6241 tgccgaagcc tgcccctgcc gcgcgcgttc agaacagcac cggcagcgcc gacgggttgc
     6301 cggacggcag cccgaagcgt tgcggatact gcacgtgcag gaaatacagg ccgtcggccg
     6361 gtgccgtgac accgccgtgg cggcggtcgc gaccggcgag cacctcggcc gcccacgcga
     6421 tcggccgctc gccgccgccg atcgcgagca gcacgccggc gatgttgcgc accatgtggt
     6481 gcaggaaggc gttggcctcg acctcgagca cgaccagatc gccgtggcgc gcgacttcga
     6541 gcgcgtacag cgcccgccgc ggatgctcgg cctggcagcc ggcagcgcgg aaggcggaaa
     6601 aatcatgctc gccgagcagg gcctgaccgg cctcgtgcat gtgcgcagca tcgagcggcc
     6661 ggtaccagtg cgtcgcccgc ccggcggtga gcgccggccg gtaacggcgg ttcaggatca
     6721 cgtagcgata gnnnnnnnnn nccgccgaga aacgcgcgtg gaagtcttcg ggcaccggct
     6781 gcgcccaggt cacggcgaca tccggcggca gctggctgtt gctgcccatg acccaggcgt
     6841 gcgcgctgcg cacagcgtcg gtgtcgaaat gcacgacctg cccggcgcca tgcacgccgg
     6901 cgtcggtgcg cccggcgcag gtcaccgcga ccggccggtc ggcgatgcgc gtcagtgcgg
     6961 cctcgagggc gccctgcacg gtgcgcacgc cctcctcctg ggtctgccag ccgcgaaagg
     7021 ccgagccgtc atattcgatg ccgagggcga tgcgcatggg tccggtcggg tcggtgagcc
     7081 gcaaaacggc aaagcatgcc gcttgcgacc ggctcaggcc agcgcgtgca tgccggcgaa
     7141 cgccagcgcg atggcgagcg gcagtcgcca ttcccgcagc gccggcagcg gcggcaccgc
     7201 cacggtgagc cggtctgccg gcgcgaagtc cgcggccggc tgtcctgcgg cggcgaacac
     7261 ggcgcgcgcc gcccccgtcc agcggcccag ccggccgctg ccggcatcgg ccggggccgt
     7321 catgcgtgcc tgcagcagcg gcagcgcctc gaaacagagc gccacgcgca ccgccagccg
     7381 ctcccggaag ccaccgccgg ccggggccag ccggtacagc gccccggcca cgccgtgcgt
     7441 cccggccagc gccagcacca gcgccgcggc agcgacgagt tcgagcagca ccagcgcgcg
     7501 ccagagcgcg agccagaggc cctgcacgct cggcgaggcg ccgccgagtg ccggcagcag
     7561 cgtcaggccc ggcgtgaacc agccatgcgt gatcacgacg gcgagcagca gccagcgcac
     7621 ccgccgcagc gcccggcgca gggtgccggc cggcagcacc ggccccgttc cccagcgggc
     7681 gaacgcgagc agcagcgccc cgagcagcag cgccggcggt gccgccggtg cgatgccgag
     7741 ggcgaacgcg acgaagcttg cgagcaggat gacgggattc atggcggcag cccttcccgg
     7801 gggtcgcgca ggggcgcggc aggatagcgg ccaaagcggc cgatcacgat ggcatcggcg
     7861 tgcttgatgg ccatcaagcc gcaccggccc ccgcctggcc gacactgcgc gcccgcctgc
     7921 gccacgccgg tgcgggccct gtcttccgga acccgccgat gcctgaatct tcctgccgta
     7981 ccgaccgcct gcgtccgctc gcgatcgcag tgctgaccgt ttccgatacc cgcacggctg
     8041 ccggggacca gtccggcgcg ctgctcgtcg agcggctgca ggacgccggt caccagctgg
     8101 tggcgcgggc gctggagccg gacgacctgt accggctgcg cgcccgcgtg tcgcagtgga
     8161 tcgccgatgc ggcggtcgag gtcgtgctgg tcaccggcgg caccgggccg accggacgcg
     8221 atcgcacgcc ggaagccctg cgcccgctgc tcgacaagga actcgaaggt ttcggggaac
     8281 tgttccggca gctctcgtac caggagatcg gtacggccgc ggcgttctcg cgggcgctgg
     8341 ccggcgtggc caacggcacg ctggtcgtgg cgctgccggg ttcgcccggc gcctgccgca
     8401 cggcctggga cggcatcctc gccgggcagc tcgacagccg ccgcgggcac tgcaactacg
     8461 tggcactgat gccgcggctc accgaaacct gagcccggac ggtggccggc ggtgcaggcg
     8521 attcagatgc cgcggccgcg gccgaggcgg aactcgacga tctcgaccag cgcggcctcc
     8581 tcggcacggc tcaggccgcc gaggcggtgc atcagcgcgc gcgcgtcgat gtgccacttg
     8641 ccgtcgagac cgcggcgctg gatttcgtgg cagacggtcg cgtacagacg gcgcagcgca
     8701 tcggtttcct cgaggttgat gtcggcgagg gcttcggaca gcgccgacca ttcggcatcg
     8761 gaaaaggccg ggcaggagtt ggcgaggatg tcctcgtagc gacgcagcag cacggcgagc
     8821 cggtgatcgg cgacgggcag ggcaacggaa gcagacatgg gggaaaacct gatcggtcgg
     8881 gtttgcactg gagagttcga tgacccgtac cgatcgcttc cgtgcgcgct gcgggccgtt
     8941 ttccggtgcg ccgcacggag gtgcggaacg ccggttttgc gtgccgctag ttctggcatg
     9001 ggtacccggc agccgcaagc cgggtcggcc ggagaaatgg tcttgtttac gattcagatc
     9061 gttacagact gttccgccac cgccgcgcgg ccatccccga tccgctgccg ccagccgcgc
     9121 cccgtccgga tcgcccgcac gaggttcagg gccagcagcc cccaggcgag cacgccggcg
     9181 gcaccggccg gccgggccag ggccggcagc gtgcaggccg ccagcgtcag ccccaccgac
     9241 aggaactggg ccgcgaactg gcgctgcagg gcggcggccg ggatcagctc gcggacgttc
     9301 ggcgccaggg cggcgctgcg gaccgcgcgc aggcgggtcg agagatccag ccagaccaga
     9361 aacgcgacga tgcgctgcag catgccggcg accagggtcg tgccgaagcc gaggagccac
     9421 agcaggccgc tcagcatttc ggtggaggaa ccgctcccgc cgctgccggt gcgcagcccg
     9481 aaccgcccgg cgagccagag gcagaccgcg agggcggcgc agccgagcgc gagcttccag
     9541 tagccgagcc acggatccgg cagccggcgc cggcgccggt gctgcagcca cagcgtgcag
     9601 gcggcgaagc cgagcagcgc cgcgccggcg gccagcgccg cggcatggca cagccagtcc
     9661 ggcagcggcc agccggcatc gaggctcgcc agccccagcg cggcgacgct caccggggca
     9721 agcccggtgc gcagccagcg cggatacggg gccgtgatcg agaacatcgg cacgacctgc
     9781 caggccaccg ccgccaccag cagtccgacc cagccgatca gcgcccagcc ggcgtgcagg
     9841 tcggtcagcc ggcgcagcag cggcacgccg gcccagccgt ggccggcggc cagcgtgaaa
     9901 ccgagcgcgg cggcgatcag gaacgacagg ccggcccagc cgatcgtgcg cgcgctctcg
     9961 ccgctgcgcg gcgagcgcca ccaggccggc agcgcagccg cggcatagac cagcagtgcg
    10021 cagagcagca gtgcgctgcc aaagcgcacg agcggtgccg cgccggtccc gagcccgccg
    10081 gcgaacagca cgatgccggg cagcagcagc gcctgcacgc cggcggcgat cggcgcagcc
    10141 cggcgcagcg gcgcaccgac gagcaccggc agcaactgga tcagcgcacc gagcatcgcg
    10201 ctcagcagca caccgagggc gagcagatgc acgtaggcca gcgcctgcgg ctgccagcgc
    10261 cccgccatca ccgccggacc ggcccagagc agccagccac cggccagcac cccgaagacc
    10321 ggcacgctca ggaaccagcg cagcggcagc gcgagcggcg gcgaggcggc gtaggagagg
    10381 ccggcgttca tgcgcagccg tcgccgtcgt catctccggc gcaggcggca ctggcgagcg
    10441 gatcgtccag ccgccagatc agcgcctcgt agcgcccggg ttccgagacc agcacgcgat
    10501 acgcgcaatc gcgttcgagc agatgcccgt acagcgggaa gggttcgcgc cggtgcagca
    10561 ggcgcagcca ctcgcccggg ccgagcgcat ccacggcatc cagcgtcgcg aacagtggtt
    10621 ccggcggctc caggccaccg acatcgagca ggtgttccgc ggtcgtcatc gtcgtgcgtc
    10681 ctgcaggcag tcaggcgctg accgccgccg ggcgcaggcc atcgagcagc gccggcaggc
    10741 gagccgcaag cagccgggcg gccatcggga acaggatctg ttcctccttc atgttgtgct
    10801 gctgcatcag cagcaccagc gtgtcggaca ggcccagcca ggcatcggca tcgcagcgct
    10861 cgccggcgcc gcgcagcgca tcgagcagcg tgcggatctg ctcgtgctca tcgcgcatga
    10921 cccgggtcgg accgcaaccg ctgccggtgg cggcctcgaa ggccgggaac agcacggctt
    10981 cctcgcgccg gaagtgggcg cgcagttcgt catcgagggc gctgccggcg gcacgggcgg
    11041 ccggccagtc gccggcggcc acctgcagtt cggcatcggc gagggccgca tccgcctggc
    11101 ggtgctggcc ttcgagcagt tcattgagcg gattcatgga catgcacgca gcaacggagg
    11161 gagtgccggg cagtctgcag agggatcgtc cgtgcgccct taacctgcat caaccacatc
    11221 gggccaccgg acggggtatg cagacaggag agtcgtgcgc ccgccggcgg ccgacctatc
    11281 atgcgcggat gccaccatcc cccgcccccg aagccctcga tcgcctgctg cgccagacct
    11341 atctgttcgc ggccctggac gagccccggc tcgccgtcgt acgccacggc atgcgcgtgc
    11401 tgcacctgca cgaaggccag cgcctgttcg atgtcggcca gccggccgag cgcttcttcc
    11461 tggtcgccag cggccagatc aagctgtttc gcgtgtcgct ggacggcaac gagaaggtca
    11521 tcgagatcgc cggccccggc aagaccttcg ccgaggcggt gatgttcatg cagcgccgtg
    11581 attatccggt cagcaccgcg gcgctgaccg aggcgcgggt ctgctcgttc gacaatcaca
    11641 cctacctcga actgctgcgc gcctcgccgt cgctctgcct gagcatgctc ggcgagatga
    11701 gcatgcgcct gcatgcgcgg ctgaacgaga tcgatcacct gacgctgcag aacgcgagct
    11761 tccgcgtcgt gcactacctg atcgaccagc tgatcgagca cggcgacggc agcggcagca
    11821 tcgatctgtc gacgccgaag aacatcatcg cctcgcgcgt atcgatcaag ccggaaacct
    11881 tctcgcgcat cctgcgcacg ctggccgacg aaggcatcgt gcgcatcgac ggcaagcaca
    11941 tcgaggtgcc ggactgccgt cgcctgcagc agtgggggcg ctgaatccgg cttcaccatc
    12001 cttaaccggg caggcgccac cggcaccgct atgctgcgcg cttcgtcccc ggggtccgcg
    12061 ccggccgggg gcgcagccgg gggtgacgga agcgccctga ccgccctgct gccgacccgc
    12121 ggcgcccagc agaagccccg gcatggttcc cagcttgcgc cagcgatcgc cgtccaccgc
    12181 ggacgacggt ctcgcgacga cggtttccaa ccacatcggt ctcgcctact tcgaggccct
    12241 cgtgcgccgg ctcgccgtcg cgctcgatgc cggcttcgcc tgcgtcgccc gccatgagcc
    12301 ggaaaccggc cgtctgcttc cggtcgcaag ccacagcacc gatgacgcgc acgactggcc
    12361 ggcggccctc gacggtgcgc cctggccgct ggtgctcgcc ggggtcacgg tgctgcaggc
    12421 cgacggggcc gatgcgctgt atgccggcaa caccgctctc gccggcgtgc gcgcctgcgc
    12481 cggcacgccg ctcgtcgatg ccggcggccg cacgctcggt ctgatcgcgg tgctcggccg
    12541 gcggccgttc gcgcagccgg cgatggttgc tctggtgctg gagctgttcg ccgagcgggc
    12601 agcggccgaa ctcgaacggc tggcggccga gcgcgaactg cagcgcaccg gggcggcgct
    12661 gcgccgccag cagctcgcgc tgctgcgcat cgtgcatgcc gagctgctgg tcgaggccga
    12721 ggccgacacc gccctcgcac gcatcgccga gatcgccggc gagaccctgg atgccgatcg
    12781 cgccgaaatc tggcaggccg atgcggcttt cggacaggtg caccggtgcg cgtcctggca
    12841 tcaccgccac ggtcgcggcc acgaccacga cggccctgcg ctcgataccc gcgactgccc
    12901 gggcttcgtc ggcacgctgc gcgcgcagat gctgctcgat ggcagcgacc cgcgccacgc
    12961 gcaggccctc gccgagtggc gggcgctcgc gccgccggat tccgaggccg gcgcctcggt
    13021 gctggtgctc ggcatcagca gcggccggac gctgcacggg ctgctgcagc tcgaatgccg
    13081 gcgcactgcg gctgcccgcg tctggggcat cgacgaactc ggcttcgcgc gctcggtctg
    13141 cgacctgctg gcgctgacgc tggtcacccg cgcgctgcgc cggaccgtcg ccgaactcga
    13201 agcctcgcag cagcgctacc gggcgctgta cgaggacatc gacacgctgg cccgcgagcg
    13261 cctgcaggcg ctcgaaaccc accagcagcg catgcaccag atgcagcgcc agttcgtgta
    13321 catggcctcg cacgagctgc gcacgccgct cgcgatcatc gacagcgccg cccagcgcat
    13381 cctgcgccgc gccgacaacc tgtcgcggac cgaactggtc gagcgcatcg gcaggatccg
    13441 cgcggccgtg tcgcgcatgg gttatctggt cgactcgacg ctcgatgtcg cgcgcttcga
    13501 aagcggcacg gtcagttacc ggcccggacc ggtcgacccc gcggcgctgc tgacgcgcgt
    13561 ctgccagcag cagcaggaac tcgccccgca ccaccgcatc gcctgccggt tcgaggcgct
    13621 gccacccagc atgagcggcg acgccggcct gctcgaacag gtgttcgcca acctgctcag
    13681 caacgccgtc aagtactcgc ccgatgccga cgagatcgag gtgcatgcct ttgccgatgc
    13741 cacgcgcctc gtcgtgcgcg tgtgcgacca cggcgtcggc atcccggctc aggagatgcc
    13801 gcggctgttc gagaagttct tccgcgccag caccgcggtc ggcatccccg gcaccggcat
    13861 cggcctgaac atcacgcgcc agatcgtcga cctgcacggc ggcatcctga cggtcgacag
    13921 ccacgtcggc accggcacca ccttcacggt caagctgccg ctgccgtccg cggcctgagc
    13981 cggaccgtcc tcctccccgg ctgcgcgccc ctccaaaaaa aagcccggca tcgaagccgg
    14041 gctcaagcaa ccaccaaagg aggagaagag aaacatcacg cggatgtcgg ggaatagtgt
    14101 cggcacgcgc ggcacacgcg gcaaacgacg gatattcacg ctggcctgaa cttttttgta
    14161 ccggacaaaa aaaggcccgg gacggcccgg gccaaaacaa ccaccaaagg aggagaagcg
    14221 acatccgggt aggatgccga agccagtttg cccgccgatg ctgcattgca caaaccatct
    14281 ggcctgatgc gcgcatgaaa aaatctgaac cgtcaggccg ggctgacaac cctgcctggc
    14341 aatccggccg ctcctccgtc ggcgcatgac gccggcttgc ggccctgagc ctcattaaaa
    14401 ttttttgatt cagtcacttg catcctccac cgctccggga tgctgacgat cccgccctgc
    14461 cagtggatac cggccgctac actggcccgt catccgtaca ccgactagac tgcgcttctg
    14521 atgcatcgca ccacagggaa gtgtcatgtc cgcgtccccc gagcctcgcc gtcccccgct
    14581 gccccgtcgt cctgtccgcc gcgccgcacc acgcggcgag gctcgtgccg gcgtactggc
    14641 gctggtcctc tgcggcgcgc tgatcgccgg tgtgatcgtt ccgtacgtgc gcgaccggct
    14701 gccggcgccg ccggctgcga gcagcgccag cgtgcagtcc ttcagcgccc cgccgcttgc
    14761 acagcggaat ctgccggcgc cggcggctcc cgttccgccg caggcaaccc ctgccccgtc
    14821 ctatccgccc gatccggtgc aggcgcgggc acggcagacg ctcgccacgc tcgccgat
//
LOCUS       DAOQIE010000050        14781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_1938_length_14781_cov_7.092150, whole genome shotgun sequence.
ACCESSION   DAOQIE010000050 DAOQIE010000000
VERSION     DAOQIE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1938_length_14781_cov_7.092150"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="atpA"
                     /locus_tag="PK144_04915"
     CDS             complement(<1..1144)
                     /gene="atpA"
                     /locus_tag="PK144_04915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNF65103.1"
                     /translation="MQLNPTEISELIRSRIEKFEPSAEARTEGTIVSLTDGIVRIHGL
                     ENVMQGEMIEFPGSTFGLALNLERDSVGAVVLGAYDHLSEGDKCFCTGRILEVPVGPE
                     LLGRVVNSLGAPIDGKGPINTSLSSPVEKVAPGVIERQSVSQPVQTGLKAIDAMVPVG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGTGVKCVYVAIGQKNSSIAAVVRKLEEHDAM
                     AHTIVVAAGASDPAAMQYLAAYSGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERASRINADEVARLTNGEVTGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGLSVSRVGGAA"
     gene            complement(1161..1715)
                     /locus_tag="PK144_04920"
     CDS             complement(1161..1715)
                     /locus_tag="PK144_04920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNF65104.1"
                     /translation="MAETTTVARPYARAAFEHAQARKGGLKKWSEVLAAAAAVAADPA
                     MQNAFADPALTVPERADVFLDVVRSVAGDKAVSEDFGNFVRLLAENRRLVLLPDIVAM
                     FEALKAEAEKSIRAEVVSAFPIGDEQKTRLAAALKQRLQREIELEVTVDESLIGGAII
                     RAGDVVIDGSARGQLANFATALRQ"
     gene            complement(1734..2204)
                     /locus_tag="PK144_04925"
     CDS             complement(1734..2204)
                     /locus_tag="PK144_04925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNF65105.1"
                     /translation="MNINATLIGQMITFALLVVFTMKYVWPPIMKAMHDRQKRIAEGL
                     ASAERGVHEQELAKARAAEMLKEAKQQAAEIIGQAQKRANEIVEQSKHEAQAEGERQL
                     HAAKAEIDLEFNRAREQLRGQVVSIAVAGAGKVLKREIDAQAHAKLLDDLVAQL"
     gene            complement(2251..2535)
                     /gene="atpE"
                     /locus_tag="PK144_04930"
     CDS             complement(2251..2535)
                     /gene="atpE"
                     /locus_tag="PK144_04930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNF65106.1"
                     /translation="MEIAKLVADVQGMTVIAVALILGLGALGTAIGFALLGGKFLEGA
                     ARQPEMIPTLQVKMFIVAGLLDAVTMIGVGIALFFTFANPFLGPVQALAH"
     gene            complement(2620..3393)
                     /gene="atpB"
                     /locus_tag="PK144_04935"
     CDS             complement(2620..3393)
                     /gene="atpB"
                     /locus_tag="PK144_04935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020405475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNF65107.1"
                     /translation="MSAGAGPHSASEYIIHHLHPMSVGEGFWTLHLDTIFFSLGLGLL
                     FLYLFRKAAVNATSGVPGGLQNFCEILVEFVDGQVKDSFHGHNPVIAPLALTIFVWVF
                     LWNSMDLLPVDLLPAIGQLFGIPYLRVVPSTDLNSTFGLSISVLCLIYYYSIKVKGGH
                     GFAHEVLGHPFGIKAAPANLLLRIVEDLAKPISLALRLFGNLYAGELIFILIALLPWY
                     VQPFLGWPWAVFHILVITLQAFIFMTLTIVYLSMAHEDH"
     gene            complement(3390..3770)
                     /locus_tag="PK144_04940"
     CDS             complement(3390..3770)
                     /locus_tag="PK144_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNF65108.1"
                     /translation="MGVNKATQRILAVQFVVTLSAVLIAAVFGDAKTVRSAGAGGGIG
                     FAVTACFAFLVFRVRPGAAAGKMARALFLGEAVKFVLTVLLFAAALVFMDLAFMPFIL
                     TYAVTLAAYWLALPLTLDASVKML"
     gene            complement(3930..4859)
                     /locus_tag="PK144_04945"
     CDS             complement(3930..4859)
                     /locus_tag="PK144_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF65109.1"
                     /translation="MAIRKGGLGRGLDALLGAARSLPAQTPAATPATPADGEEAQADG
                     LLRRLPVELVGRGRWQPRLDIDAGALEELAASIRAQGVVQPIVVRALDDGRYEIIAGE
                     RRWRAAQLAGLAEIPALVREVPDHAALAIALIENIQREDLNPLEEALALRRLIDEYDL
                     THQAVADAIGRSRAAVSNLLRLLELAPEARELLRSGRIEMGHARALLTLPSALQVEAA
                     RQVALRGLNVRETEALARRLSGTTAAAGHPQAAPDPDVRRLQDRLSSRLGARVQLRHG
                     NGGKGSLVIHYNTLDELDGIIAHFGQDDFPLSS"
     gene            complement(4874..5662)
                     /locus_tag="PK144_04950"
     CDS             complement(4874..5662)
                     /locus_tag="PK144_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF65110.1"
                     /translation="MGKVIAIANQKGGVGKTTTAVNLAAALAAAKVRLKIILIDLDPQ
                     GNATMGCGIDKHGAEATMTEVLLGETSIGEALVYSESAGIQVLPSNGDLTAAEIGLAT
                     REQGAFRLRETLAAIRGNFDYIVIDCPPSLNMLTINALTAADSILIPVQCEYYALEGL
                     SSLLSTIEHVRASTNPQLHIEGLLRTMFDARNRLANEVSGQVLEHFAGQVYDAIIPRT
                     VRLAEAPSYGQPIIVYDPASRGAESYRELAREVLKRARRPAAAV"
     gene            complement(5688..6323)
                     /gene="rsmG"
                     /locus_tag="PK144_04955"
     CDS             complement(5688..6323)
                     /gene="rsmG"
                     /locus_tag="PK144_04955"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNF65111.1"
                     /translation="MKLEDALADGLGRLGLCPPSPAITALAAYLRLLEQWNRAYNLTA
                     VRELGDMVAKHVLDSAAVLPWLHGARVLDVGSGAGLPGIPLAILSPERDYVLLDGLGK
                     RTRFLTQVVLELKLAHVQVVQARVEDWQAPAPFTSVISRAFAALPEFVELAGPHCASD
                     GRLLAMKGQLDAAETAAVPPGWRLRATHRLDVPGINGERHLLELEPLAAAA"
     gene            complement(6396..6863)
                     /locus_tag="PK144_04960"
     CDS             complement(6396..6863)
                     /locus_tag="PK144_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65112.1"
                     /translation="MALTLALLLAVHLLAASFWLGGMAFMLWIAPPALAARLPPEQQG
                     PLRLALLGRFLAAVWIAMPLTLASGYALFALTGAAVRASPGLHAMAGLGTLMGAVFAG
                     LYFGVFRPLRAAAAAGERERAVRLAGRLRKLVLLNLGFGTLVVLAAALARYGH"
     gene            complement(6879..7814)
                     /locus_tag="PK144_04965"
     CDS             complement(6879..7814)
                     /locus_tag="PK144_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF65113.1"
                     /translation="MDFDHGISAIDTGFERPLFDASHLIIDHGRAAFVDAGVNASVPM
                     LIGVLEHKGLTPAAVDWLLLTHVHLDHAGGAGELLRRLPNAKLVVHPRGVRHMVDPTA
                     LMAGASAVYGEDVVRRTYGTLTPVPPGRIVEATEGLELVLGSRRLTVFDTPGHARHHV
                     CYRDHGASAFFTGDTFGISYREFDTARGAFIFPTSTPVQFEPEAMKASIARMAACQPE
                     AMYLTHYSRVTDVPRLAAELIEQVDQLVALAQAADGHADRHERLVESLHALFAARAAA
                     HGCTLGAAQVRELLALDAELNAQGLEVWLDRARAA"
     gene            8160..8747
                     /locus_tag="PK144_04970"
     CDS             8160..8747
                     /locus_tag="PK144_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65114.1"
                     /translation="MVTIQETPFAGLEADGRLLSPVHKGPTKKPGRFGFRGVIALEFA
                     EKMSDEARPPSIKADQVMAVGDAGASSLPFVAAMTLSAKHLPLLVEVLGERLVPEGKY
                     FLFATDVDFSERWQLSYNGIPFWVLPLDEATVYNEVLELVGVEKSDLKKLDTAGKLDA
                     VADAAAGFSDSFPAISYEDLVARLGSVRVNEYRPV"
     gene            complement(8772..8948)
                     /locus_tag="PK144_04975"
     CDS             complement(8772..8948)
                     /locus_tag="PK144_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65115.1"
                     /translation="MDTGRRRYPFIWFERGGWWFKIFGEVFGPFANATRAHDSFVLAR
                     RLDSGWYGEGRDAR"
     gene            complement(9039..10016)
                     /locus_tag="PK144_04980"
     CDS             complement(9039..10016)
                     /locus_tag="PK144_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008883971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNF65116.1"
                     /translation="MNAVGIRTPGGPEVLQPCTRPVPQPRAGEVLIEVAAAGVNRPDC
                     LQRAGAYPPPPGASDLPGLEVAGCVVAVGTGVDWPAAGEHVCALCNGGGYAGYVAVPA
                     GQCLPVPAGWSDIEAAALPETFFTVWTNLFERGRLAAGESVLVHGGAGGIGSTAIRLA
                     TAFGATVYASAGSAEKCAACLAAGAARAIDHTREDFVTVVRSETGGRGVDVILDIVGA
                     GYLARHLDLLAPEGRLVLIAGLQGYKAELNLWPLLAKRLSITGSTLRARSDADKAAIA
                     TALRERVWPLLDAGRLRPPPPATLALAQAAEAHRRMEANSLIGKLVLLP"
     gene            10181..11374
                     /locus_tag="PK144_04985"
     CDS             10181..11374
                     /locus_tag="PK144_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65117.1"
                     /translation="MAELRRILFVDNENTVLRELETGLAAMRGQWQMEFISDARAALQ
                     RLDEERFDALIADPCLSGKDGASLLAEAAQRHPPLVRIVLAGPSERSAALDALAVAHQ
                     VLAKPCDPAELRATIGRALALRKFLGGDTPLKALVSRLDSLPSMPALYRRIVEELQSA
                     HASFESVAAIIGQDPPMAAKILQLVNSAYFTRAQHVSDLSQALRMLGLDTIKALVLSL
                     QVFSQFDAETLAGLSVERIWRHSVRTGALARAIARAERAPREVENDAFTAALLHDVGK
                     LILAANLPNIYAKIGPLCDAEALEPCAAERRLFGAAHAETGAYLLGLWGLPESIVEAV
                     AYHHHPRDREPAGICALTWVHAANALAQLPDAPVGVLDAEYLGALDLLERLPAWAALG
                     EAMPD"
     gene            11517..11918
                     /locus_tag="PK144_04990"
     CDS             11517..11918
                     /locus_tag="PK144_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF65118.1"
                     /translation="MTSTLRAARLLAAACLIAGLLTGCAGSGPSGPDSYEAAQTQFRS
                     RVQQAWVAPKSFAKTPRALSTTVRISSDATGAVKEVKMIRRSGRRDFDASVVKAVLDA
                     SPLPVAQDKALATRLQSLELELGTDAQGRMR"
     gene            12033..14744
                     /locus_tag="PK144_04995"
     CDS             12033..14744
                     /locus_tag="PK144_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNF65119.1"
                     /translation="MTAGIPRVADWHAQTPDSVRTTLATPDTGLTAADAAGRLEHHGP
                     NRLPPPKTVGPWKRFLRQFDNVLIYVLLASAATTAALGHPVDTAVILGVVLINAIIGF
                     VQEGRAESALAAIRDLLSLRACVLRDGERREIAAEELVPGDRVLLASGDKVPADLRLV
                     SVRSLRVQEAALTGESEPVEKDTAAVAADAALGDRRCMAYSGTLVTYGQAQGLVVATG
                     ADTEIGRISALLAQVEPLTTPLLEQINRFGRWLTGVILILAAATFLFGWLVRGLPWPD
                     MFLAAVGLAVAAVPEGLPAVMTITFAIGMQRMARRRAIVRRLPAVETLGSVTVICSDK
                     TGTLTQNAMTVKSVAAPGGETWMVEGVGYAPEGHLSRNGEPVEAATVGAALAIARAGQ
                     LCNDARLRRSEAGDWSIEGDPTEGALLTLARKLGIDIQALEVAQPRLDSIPFESEHRY
                     MATLHRDGDGARLFVKGAPERVLGMCADERHGDGVRALAGDWQARIDALAALGQRVLA
                     LAERRFDTTPDELTHELAGSGLTLLGLLGIIDPPRPEAVAAVHDCHTAGVRVKMITGD
                     HAITARAIATELGLGPNGRVMTGAEVERLDDEVLRAAVADTDVYARASPEHKLRLVAA
                     LQANREVVAMTGDGVNDAPALKRADVGVAMGANGTEAAKEAAAVVLADDNFATIARAV
                     EEGRTIYDNLKKAIVFSLPTNGAQACVILAAIAFGVALPVTPVQVLWVNMVVAVTLSL
                     TLAFEPSESDVMRRPPRDRDAALISGFLAWRVALVCVVQTIGSLGLFLWETASGAPVE
                     QARTLAVNALVVGQIFYLFNSRYTVAPSTSLAGLTGNRVALLGIAILLGLQMAFTYVP
                     LMQTLLGTAALGAREWLLALGVGASVYVVVELEKWALRARLAHRGAG"
BASE COUNT         2252 a         5091 c         5214 g         2224 t
ORIGIN      
        1 gcgccgcacc gccgacgcgc gacaccgaca gaccggcgtt gatcgcgggc cggatgccgg
       61 cgttgaacag gtcggtttcc aggaagatct ggccgtcggt gatcgagatg acgttggtcg
      121 gcacgaacgc ggacacgtcg ccggcctgcg tctcgatgac cggcagtgcg gtcagcgagc
      181 cggtcttgcc ggtgacttcg ccgttggtca ggcgcgcgac ttcgtcggcg ttgatgcgcg
      241 aggcgcgctc cagcagacgc gagtgcaggt agaacacgtc gcccggatag gcttcgcgac
      301 ccggcggacg gcgcagcagc agcgagatct ggcggtaggc gacggcctgc ttggacaggt
      361 cgtcatagac gatcagcgcg tcctcgccgc ggtcgcggaa gtactcgccc atcgcgcagc
      421 ccgagtaggc ggccaggtac tgcatggcgg ccggatcgga ggcaccggcg gcgacgacga
      481 tggtgtgggc catcgcatcg tgctcttcga gcttgcgcac cacggcggcg atcgacgagt
      541 tcttctggcc gatcgcgacg tagacgcact taacgcccgt gcccttctgg ttgatgatcg
      601 cgtcgatcgc cacggcggtc ttgccggtct ggcggtcgcc gatgatcagc tcgcgctggc
      661 cgcggccgac cgggaccatc gcgtcgatcg ccttgaggcc ggtctgcacc ggctggctga
      721 ccgactggcg ctcgatgacg cccggggcga ccttctcgac cggcgagctc agcgaggtgt
      781 tgatcgggcc cttgccgtcg atcggcgcgc cgagcgagtt gacgacgcgt ccgagcagtt
      841 cggggccgac cggcacttcg aggatgcggc cggtgcagaa gcacttgtcg ccctcggaca
      901 ggtgatcgta ggcgccgagc accaccgcac cgacggagtc gcgctccagg ttcagggcga
      961 ggccgaaggt gctgcccggg aactcgatca tctcgccctg catcacgttc tcgagaccgt
     1021 gaatgcggac gatgccgtcg gtgaggctga cgatcgtgcc ttccgtgcgt gcctcggccg
     1081 acggctcgaa cttctcgatg cggctccgga tcagttcact gatttcggta ggattcagtt
     1141 gcataagcct tcaattcccc tcactggcgc agggcggtgg cgaagttcgc cagctggccc
     1201 cgagctgaac cgtcaatgac cacgtccccg gcccggatga tcgcgccgcc gatcagcgat
     1261 tcgtcgaccg tgacttccag ttcgatctcg cgctgcagcc gctgcttgag cgcggctgca
     1321 aggcgggtct tctgttcgtc gccgatcggg aaggccgaga ccacctcggc gcggatcgac
     1381 ttctcggcct cggccttcag tgcctcgaac atcgcgacga tgtcgggcag cagcacgagg
     1441 cgccggttct ccgccagcag gcggacgaag ttgccgaaat cctcggagac cgccttgtcg
     1501 ccggccaccg agcgcaccac gtccagaaac acatccgcac gctcgggcac cgtgagggcc
     1561 ggatcggcga aggcgttctg catggccgga tcggccgcga cggccgcagc cgccgcgagc
     1621 acttccgacc acttcttcaa gccgcccttc ctggcctggg cgtgctcgaa cgcagcgcgg
     1681 gcgtaggggc gtgcgacagt tgtcgtttca gccatgggta gtcccgtccg ttgtcagagc
     1741 tgagcgacca gatcgtcgag cagcttggca tgggcctggg cgtcgatttc acgcttcagg
     1801 accttgccgg caccggcaac ggcgatgctg accacctggc cacgcaactg ctcgcgggca
     1861 cggttgaact cgagatcgat ctcggccttg gccgcgtgca gctgacgctc gccttcggcc
     1921 tgcgcctcgt gcttggactg ctcgacgatc tcgttggccc gcttctgggc ctggccgatg
     1981 atctcggccg cctgctgctt ggcttccttc agcatctcgg cagcacgggc cttggccagt
     2041 tcctgctcgt ggacgccgcg ttcagcggaa gccagacctt cggcaatacg cttctggcga
     2101 tcgtgcatgg ccttcatgat cggaggccat acgtacttca tcgtgaagac caccagaagg
     2161 gcgaacgtga tcatctgccc gatgagcgtg gcattgatat tcacgtcatt tcccccttgt
     2221 acgggtcatg gaacccggat ggccttcgga ttagtgcgcc agagcctgga ccgggccgag
     2281 gaaggggttc gcgaacgtga agaacagggc gataccgacg ccgatcatgg tcaccgcgtc
     2341 gagcaggccg gcgacgatga acatcttgac ctgcagggtc gggatcattt ccggctgacg
     2401 cgcggcgcct tccaggaact tgccaccgag cagcgcgaag ccgatggcgg tacccagcgc
     2461 acccagaccc aggatcagag caacggcaat gacggtcatg ccctggacgt cagcaacgag
     2521 cttggcgatt tccatttttc ctccagacgt gaagcgggat tggattgagt tgaaacaaag
     2581 ggactgaggt ttacttaccg actacgactt ccgacccggt cagtgatcct cgtgcgccat
     2641 cgacaggtac acgatcgtca gggtcatgaa gatgaaggcc tgcagggtga tgaccaggat
     2701 atggaacacc gcccacggcc agccgaggaa gggctggacg taccacggca gcagcgcgat
     2761 caggatgaag atcagttcgc cggcatacag gttgccgaac agacgcagtg cgagcgaaat
     2821 cggcttggca aggtcttcga cgatgcgcag cagcaggttg gccggcgcag ccttgatgcc
     2881 gaacggatgg ccgagcacct cgtgcgcgaa accgtgaccg cccttgacct tgatgctgta
     2941 gtagtagatc aggcacagca cgctgatcga caggccgaag gtcgagttca ggtcggtcga
     3001 cggcacgacg cgcaggtacg ggatgccgaa cagctgaccg atggccggca gcaggtcgac
     3061 cggcagcagg tccatggaat tccacaggaa tacccagacg aaaatcgtca gcgccagcgg
     3121 cgcgatgacc gggttgtgac cgtggaatga atccttgacc tggccatcga cgaattcgac
     3181 gaggatttcg cagaaattct gcagcccgcc cggcacgccg gacgtggcat tcacggccgc
     3241 cttgcggaac aggtacagga acagcaggcc gagtccgagc gagaagaaga tcgtgtcgag
     3301 gtgaagggtc cagaaaccct cgccgacgga catcggatgg aggtgatgaa tgatgtattc
     3361 cgaagcggaa tgaggacctg cacctgcact catagcatct tcaccgaagc gtccaaagtc
     3421 agaggtagag ccagccagta agctgccagc gtcaccgcat aggtgaggat gaagggcatg
     3481 aatgccagat ccatgaacac cagtgccgcc gcaaacagca gcaccgtgag cacgaatttg
     3541 accgcttcgc ccagaaacag cgcacgcgcc attttcccgg ccgcggcgcc ggggcgcacg
     3601 cgaaacacga gaaaggcaaa gcacgctgtg acggcgaaac cgatcccccc gcccgcacct
     3661 gctgaacgta ccgttttcgc atcgccgaaa acggccgcga tcagcacggc cgacaacgta
     3721 accacaaact gaacagcaag tatcctttgc gttgccttgt tgacgcccat gagctcggtt
     3781 ttcttgtcgc ttctggtagc cgggatactg ttgtcgtccg atctcgccgt tccgttattt
     3841 gaaaacggcg tttcgtatgg caacatgctc caggccgggc gtcccgcaaa cgcggcgcgc
     3901 attataaaag tgcgtggcgc accggtcaat caagaactta acggaaagtc atcctgcccg
     3961 aagtgcgcga tgatgccgtc gagttcatcc aaggtattgt aatgaatgac caaactgccc
     4021 ttgccgccat tgccgtgacg cagttgcacg cgtgcgccga gccggctgga cagtcgatcc
     4081 tgcaggcggc gcacgtcggg gtcgggcgcc gcctgcggat gccccgcagc ggccgttgtg
     4141 ccgctcagcc gccgcgccag cgcttccgtc tcgcgcacgt tcagtccgcg cagcgcaacc
     4201 tgacgcgccg cctcgacctg cagcgcggac ggcagcgtca gcagcgcgcg ggcgtggccc
     4261 atctcgatgc ggccgctgcg cagcagctcg cgcgcctcgg gcgcgagttc gagcaggcgc
     4321 agcaggttcg agaccgcggc ccgcgagcgg ccgatcgcat cggcgacggc ctggtgcgtc
     4381 aggtcgtact cgtcgatcag ccggcgcagg gccagtgcct cttcgagggg gttgaggtcc
     4441 tcgcgctgga tgttctcgat cagcgcgatc gcgagcgccg cgtgatcggg cacctcgcgc
     4501 accagcgccg ggatctcggc gaggccggcg agctgcgcgg cccgccagcg ccgctcgccg
     4561 gcgatgatct cgtagcgccc gtcatcgagc gcccgcacga cgatcggctg cacgacgccc
     4621 tgcgcgcgga tcgacgcggc gagttcctcc agcgcaccgg cgtcgatgtc gagccgcggc
     4681 tgccagcgtc cgcgtccgac gagttcgacc ggcagccggc gcagcaggcc gtccgcctgc
     4741 gcttcttcgc cgtcagccgg cgttgccggt gtcgctgccg gtgtctgcgc cggcaggctg
     4801 cgtgccgcgc cgagcagcgc atcgagtccg cggcccagtc cgcccttcct gatcgccatg
     4861 tgctgcatgc tcctcagacg gcggcggccg ggcggcgggc gcgcttgagc acctcgcgcg
     4921 ccagttcgcg gtagctttcg gcgccgcgcg aggccgggtc gtagacgatg atcggctggc
     4981 cgtagctcgg ggcctcggcc aggcgcacgg tgcgcgggat gatcgcgtca tagacctggc
     5041 cggcgaagtg ttcgagcacc tggccggaga cctcgttggc gaggcggttg cgggcatcga
     5101 acatcgtgcg cagcagtcct tctatatgga gttgcggatt ggtgctggcg cgcacgtgct
     5161 cgatcgtcga cagcagcgag gacaggcctt cgagcgcgta gtactcgcac tgcaccggga
     5221 tcaggatgct gtcggcggcg gtcagcgcgt tgatcgtcag catgttcagc gacggcgggc
     5281 agtcgatgac gatgtaatcg aagttgccgc ggatcgccgc gagcgtctcg cgcaggcgga
     5341 acgcgccctg ctcgcgggtc gcgaggccga tctcggcggc ggtcaggtcg ccgttggacg
     5401 gcagcacctg gatgccggcc gattccgagt acaccagcgc ctcgccgatc gaggtctcgc
     5461 cgagcagcac ctcggtcatc gtcgcctcgg cgccgtgctt gtcgatgccg cagcccatgg
     5521 tggcgttacc ctgcggatcg aggtcgatca ggatgatctt cagccgcacc ttggcggcgg
     5581 cgagggcggc ggcgaggttg acggcggtgg tggtcttgcc gaccccgccc ttctggttcg
     5641 cgatggcgat gaccttgccc atgcgtgaat caccggtgat gaagaattca ggcggccgca
     5701 gcgagcggtt cgagttcgag cagatgacgc tcgccgttga tgcccggcac gtcgagccgg
     5761 tgggttgccc gcaaccgcca gccgggcgga accgcggcgg tctcggcagc gtcgagctgg
     5821 cccttcatcg ccagcagccg gccgtcgctc gcgcagtgcg gcccggcgag ttcgacgaac
     5881 tccggcaggg ccgcgaaagc ccgcgagatc acgctcgtga acggcgccgg tgcctgccag
     5941 tcctcgacgc gcgcctgcac gacctgcacg tgcgcgagct tcagctccag caccacctgc
     6001 gtcaggaagc gcgtgcgctt gccgagtccg tcgagcagca cgtagtcgcg ctccgggctc
     6061 aggatcgcca gcgggatgcc gggcaggccg gcgccgctgc cgacatcgag cacgcgcgca
     6121 ccgtgcagcc acggcagcac cgcggcgctg tcgagcacgt gcttcgccac catgtcgcca
     6181 agctcgcgca cggcggtcag gttgtaggcg cggttccact gttcgagcag gcgcagatag
     6241 gcggcgagtg cggtgatggc cggcgacggc gggcacaggc cgaggcggcc gaggccgtca
     6301 gcgagagcgt cttcgagctt catgcgggcg atcggggacg gcagcggacg agccggcgga
     6361 gtgtatcacg gccgccgcgg gcggccggcg acggctcagt gaccgtagcg ggccagcgcc
     6421 gcggccagca cgaccagcgt gccgaagccg aggttgagca gcacgagctt gcgcagccgg
     6481 ccggcgagcc ggacggcccg ctcgcgctcg ccggcggcgg ccgcggcgcg cagcggccgg
     6541 aacacgccga aataaagccc ggcgaacacg gcgcccatca gcgtgccgag cccggccatc
     6601 gcgtgcaggc ccgggcttgc gcgcacggca gcgccggtca gcgcgaacag cgcgtagccg
     6661 gaggcgagcg tcagcggcat cgcgatccag accgcggcga ggaagcgccc gagcagcgcc
     6721 aggcgcagcg gcccctgctg ctcgggcggc aggcgcgcgg ccagcgccgg gggcgcgatc
     6781 cacagcatga aggccatgcc gccgagccag aagctcgcgg cgagcaggtg tacggcgagc
     6841 agcagcgcga gcgtcagggc catgtcgggc gcaccggttc aggccgcgcg cgcgcggtcg
     6901 agccagactt cgaggccctg cgcgttcagc tcggcgtcga gcgcgagcag ctcacgcacc
     6961 tgcgcggcac cgagcgtgca gccgtgcgcg gcggcgcgtg cggcgaacag cgcatgcagg
     7021 ctctcgacca ggcgctcgtg gcggtcggcg tgaccgtcgg ccgcctgcgc cagcgcgacc
     7081 agctgatcga cctgttcgat gagttcggcg gcgagtcgcg gcacgtcggt gacgcgcgag
     7141 taatgcgtca ggtacatcgc ctcgggctgg caggcggcca tgcgtgcgat cgaggccttc
     7201 atcgcttcgg gctcgaactg caccggcgtc gaggtcggga agatgaacgc gccgcgcgcg
     7261 gtgtcgaatt cccggtacga gatgccgaag gtgtcgccgg tgaagaaggc gctggcgccg
     7321 tggtcgcggt agcagacgtg atggcgggcg tggccggggg tgtcgaacac cgtcagccgg
     7381 cggctgccga gcacgagttc gaggccctcg gtggcctcga cgatgcgccc gggcggcacc
     7441 ggcgtcagcg tgccgtaggt gcgccgcacc acgtcctcgc cgtagacggc cgaggcgccg
     7501 gccatcagcg ccgtcggatc gaccatgtgg cgcacgccgc gcggatgcac gaccagcttc
     7561 gcgttcggca gccggcgcag cagctcgccg gcgccgccgg cgtggtcgag gtggacgtgc
     7621 gtcagcagca gccagtcgac cgcggcgggc gtgaggccct tgtgttcgag cacgccgatc
     7681 agcatcggca ccgaggcgtt gacgccggca tcgacgaagg cggcccggcc gtggtcgatg
     7741 atcaggtggc tcgcgtcgaa cagcgggcgt tcgaagccgg tgtcgatcgc gctgatgccg
     7801 tggtcgaaat ccagaaagct cagcgtcccg tggtcgagac gctcgatggc gacggcggtc
     7861 ggtggattca cgcggggccc cgggcggtcg gcagatctgc aggagtgccg agtgtaaccg
     7921 catcctccgc ggcccggacc gcccgccggc ggctgtcgca aacccgacaa gtcgcggagc
     7981 ttgtcggcaa gccccccggg cgatgccggg tcgctcagga gggcggagtt ttcatctgat
     8041 tgatgaagat ggataaaacc ggtttttcac agaatggtat ggaccttgct caaccccgat
     8101 ctgcggcgcg tgcgccccag ttttcgtacc tcgtccatac catttccggg agaccctcca
     8161 tggtcacgat tcaggaaacc cccttcgccg gcctggaggc cgacgggcga ctgctgtcgc
     8221 cggtgcacaa gggcccgacc aagaagccgg ggcgcttcgg cttccgcggc gtgatcgcgc
     8281 tcgaattcgc cgagaagatg tccgacgaag cccgcccgcc gtccatcaag gccgatcagg
     8341 tcatggcggt cggtgatgcc ggggcgagca gcctgccgtt cgtcgcggcg atgacgctgt
     8401 cggcgaagca tctgccgctg ctggtcgagg tgctcggcga gcggctggtg cccgagggca
     8461 agtacttcct gttcgcgacc gacgtcgatt tctccgagcg ctggcagctc agctacaacg
     8521 gcatcccgtt ctgggtgctg ccgctcgacg aggccaccgt ctacaacgaa gtgctcgaac
     8581 tcgtcggcgt cgagaagagc gacctgaaga agctcgacac cgccggcaag ctcgatgcgg
     8641 tcgccgacgc cgccgccggc ttctcggaca gcttcccggc gatcagctac gaggacctcg
     8701 tcgcccgcct cggcagcgtg cgcgtcaacg agtaccgccc ggtctgatcc ggcgcacggg
     8761 acgcgcagcc ttcagcgcgc gtcccggcct tcgccgtacc agccgctgtc gagccggcgt
     8821 gccagcacga agctgtcgtg cgcgcgcgtc gcgttcgcga acggcccgaa cacttccccg
     8881 aagatcttga accaccagcc cccccgttcg aaccagatga aggggtagcg cctgcgtccc
     8941 gtgtccactg ccgcctccgc cgcctgtgcg gcccgctgtg catggcaagt accatgtctg
     9001 cggataacgg ccgggccggc ggctacttga gcccggcttc agggcagcag caccagcttg
     9061 ccgatcaggc tgttggcctc catgcgccgg tgcgcctcgg cggcctgcgc gagcgcgagc
     9121 gtcgccggtg gcggcggacg cagccggccg gcgtcgagca gcggccagac ccgctcgcgc
     9181 agcgccgtgg cgatcgcggc cttgtcggcg tcgctgcgcg cgcgcagcgt cgagccggtg
     9241 atgctcaggc gcttcgcgag cagcggccag aggttcagct cggccttgta gccctgcagg
     9301 ccggcgatca gcaccagccg gccctcgggc gccagcaggt cgagatgacg cgccagatag
     9361 ccggcaccga cgatgtcgag gatcacgtcg acaccgcgcc cgccggtttc gctgcgcacg
     9421 acggtgacga agtcctcgcg cgtgtgatcg atcgcgcgcg cagcaccggc ggcgaggcag
     9481 gccgcgcatt tttccgcgct gccggcgctc gcgtagaccg tggcgccgaa ggccgtcgcc
     9541 agccggatcg cggtcgagcc gatgccgccg gccccgccgt gcacgagcac gctttcgccg
     9601 gcggcgagcc ggccgcgctc gaacaggttg gtccagaccg tgaagaaggt ctcgggcagc
     9661 gccgcggcct cgatgtcgct ccagcccgcc ggcaccggca ggcactggcc ggccggcacc
     9721 gcgacgtagc cggcgtagcc gccgccgttg cagagcgcgc agacgtgctc gccggccgct
     9781 ggccagtcga cgccggtgcc gacggcgacg acgcagccgg cgacttccag ccccggcagg
     9841 tccgaggcgc cgggcggcgg cgggtaggcg ccggcgcgct gcaggcagtc cgggcggttg
     9901 acgccggccg cggcgacctc gatcagcacc tcgccggcgc gcggctgcgg caccggtcgc
     9961 gtgcacggct gcagcacctc ggggccgccg ggcgtgcgga tcccgacggc gttcatcgtg
    10021 gccggcacgg caggcatggg cggcatccgg gtgagggtgg gcggccgcga gtattgaggg
    10081 tcgccggggg ctgcgacaag cgcggtgggc gtgacacttt gcgttacgat gcccgccgcc
    10141 gcggcatccg gcccggctgg cagcggagcg gaatctgacg atggcggagt tgcggcgcat
    10201 cctgttcgtc gacaacgaga acacggtcct gcgtgagctg gagaccgggc tcgcggccat
    10261 gcgcgggcag tggcagatgg aattcatcag cgatgcgcgc gccgcgctgc agcgcctcga
    10321 cgaggagcgc ttcgatgcgc tgatcgccga tccctgcctg agcggcaagg acggcgcaag
    10381 cctgcttgcc gaggccgcgc agcgccatcc gccgctggtg cgcatcgtgc tcgccgggcc
    10441 gtcggagcgc agcgccgccc tcgatgcact cgccgtcgcg catcaggtgc tcgcgaagcc
    10501 ctgcgatccg gccgagctgc gcgcgaccat cggccgggcg ctggcgctgc gcaagttcct
    10561 cggtggcgac acgccgctga aggcgctggt gtcgcggctc gactcgctgc cgagcatgcc
    10621 ggcgctgtac cggcgcatcg tcgaggagct gcagtcggcg catgcctcgt tcgagtcggt
    10681 ggcggcgatc atcggccagg acccgccgat ggccgcgaag atcctgcagc tcgtgaactc
    10741 ggcctacttc acgcgcgccc agcacgtcag cgacctgtcg caggcactgc gcatgctcgg
    10801 cctcgacacg atcaaggcgc tggtgctgtc gctgcaggtg ttctcgcagt tcgacgccga
    10861 gacgctggcc gggctgtcgg tcgagcgcat ctggcgccac agcgtgcgca ccggcgcgct
    10921 ggcccgcgcc atcgcccgcg ccgagcgcgc gccgcgcgag gtcgagaacg acgccttcac
    10981 cgccgcgctg ctgcacgatg tcggcaagct gatcctcgcc gccaacctgc cgaacatcta
    11041 cgcgaagatc gggccgctct gcgatgccga ggccctggaa ccctgcgccg ccgaacggcg
    11101 gctgttcggt gccgcgcacg ccgagaccgg cgcctacctg ctcgggctgt gggggctgcc
    11161 ggagagcatc gtcgaggcgg tcgcctacca ccatcacccg cgcgaccgcg aaccggccgg
    11221 catctgcgcg ctgacctggg tgcatgccgc caacgcgctg gcgcagctgc cggatgcccc
    11281 ggtcggcgtg ctcgatgccg agtacctcgg cgcgctcgac ctgctcgaac gcctgccggc
    11341 ctgggctgcg ctcggcgagg ccatgcccga ctgatcgccg gggcggggcg gcggcggcct
    11401 tcgcttgtcg cccgtcgatg gctgccgcta ggctgtgccg ctttcctgcc cggcgccgtg
    11461 ccccggtcgt gcggccggcg ccctcgatgg atcgtccacc cggaacctgc aagcccatga
    11521 ccagcaccct gcgcgcggcc cgcctgctcg ccgccgcctg tctcatcgcc ggactcctca
    11581 ccggctgcgc cggcagcggc ccgagcggcc ccgacagcta cgaagccgcc cagacccagt
    11641 tccgcagccg cgtgcagcag gcctgggtcg caccgaagtc gttcgcgaag acgccgcggg
    11701 cactgtcgac gaccgtgcgc atcagcagcg atgccaccgg cgcggtcaag gaagtgaaga
    11761 tgatccggcg cagcggccgg cgcgacttcg acgcctcggt ggtcaaggcc gtgctcgacg
    11821 cctcgccgct gccggtggcg caggacaagg cgctggccac gcgcctgcag agcctggaac
    11881 tggagctcgg caccgacgcg cagggccgga tgcgctgatc ccagtccatc cctgccggaa
    11941 attccggcgc cggcgtatcc ggcagcggtg aaccgggcga tgatcgtcca ctcttacagg
    12001 tgggatttct ccacccacct gcagggggca ccatgactgc cggcatcccg cgcgtcgccg
    12061 actggcacgc gcaaacgccc gattccgtcc gcacgaccct cgccacgccc gacaccgggc
    12121 tcaccgccgc cgacgcggcc gggcggctcg aacatcacgg cccgaaccgc ctgccgccgc
    12181 cgaagacggt cgggccgtgg aagcgcttcc tgcgccagtt cgacaacgtg ctgatctacg
    12241 tgctgctcgc ctcggcggcg accacggccg cgctcggaca tccggtcgac accgccgtga
    12301 tcctcggcgt cgtgctgatc aacgccatca tcggtttcgt gcaggaaggc cgcgccgaat
    12361 cggcgctggc ggcgatccgc gacctgctgt ccctgcgcgc ctgcgtgctg cgtgacggcg
    12421 aacggcgcga gatcgccgcc gaggaactcg tgcccggcga ccgggtgctg ctcgccagcg
    12481 gcgacaaggt gccggccgac ctgcgcctgg tgtcggtccg gagcctgcgc gtgcaggagg
    12541 cggcgctgac cggcgagtcc gagccggtcg agaaggacac ggccgccgtc gcggccgacg
    12601 ccgcactcgg cgaccgccgc tgcatggcct attccggcac gctggtcacc tacggtcagg
    12661 cgcaggggct ggtggtcgcc accggtgccg ataccgagat cggccgcatc agcgcactgc
    12721 tcgcccaggt cgagccgctg accacgccgc tgctcgaaca gatcaaccgc ttcggccgct
    12781 ggctgaccgg cgtgatcctg atcctcgccg ccgcgacctt cctgttcggc tggctggtgc
    12841 gcggcctgcc gtggccggac atgttcctcg ccgcggtcgg cctcgcggtc gccgccgtgc
    12901 ccgagggcct gccggcggtc atgaccatca ccttcgcgat cggcatgcag cgcatggcgc
    12961 gccggcgcgc gatcgtgcgc cggctgccgg cggtcgagac gctcggctcg gtgacggtga
    13021 tctgctcgga caagaccggc acgctgacgc agaacgcgat gacggtgaag agcgttgcgg
    13081 cgcccggcgg cgagacctgg atggtcgagg gcgtcggcta tgcgcccgaa gggcatctga
    13141 gccgcaacgg cgagccggtc gaagcggcca ccgtcggggc ggcgcttgcg atcgcccgcg
    13201 ccgggcagct ctgcaacgac gcgcgcctgc gccgcagcga ggccggcgac tggagcatcg
    13261 agggcgatcc gaccgagggc gcgctgctga cgctggcgcg caagctcggc atcgacatcc
    13321 aggcgctcga agtcgcgcag ccgcggctcg acagcatccc gttcgagtcc gaacaccgct
    13381 acatggccac cctgcaccgt gacggcgacg gcgcgcgcct gttcgtcaag ggcgcgccgg
    13441 agcgcgtgct cggcatgtgt gccgacgagc gtcatggcga cggcgtgcgt gcgctcgccg
    13501 gcgactggca ggcgcgcatc gatgcgctcg ccgcgctcgg tcagcgcgtg ctcgcgctcg
    13561 ccgagcgccg cttcgacacg acgccggatg agctgacgca cgagctggcc ggatcgggcc
    13621 tgacgctgct cggcctgctc ggcatcatcg acccgccgcg tccggaggcg gtcgccgccg
    13681 tgcatgactg ccacacggcc ggcgtgcgcg tgaagatgat caccggcgat cacgcgatca
    13741 ccgcgcgcgc gatcgcgacc gaactcggcc tcggcccgaa cggcagggtc atgaccggcg
    13801 ccgaggtcga gcgcctcgac gatgaggtcc tgcgcgccgc ggttgccgac accgatgtct
    13861 atgcccgcgc cagccccgag cacaagctca ggctggtcgc cgcgctgcag gccaaccgcg
    13921 aggtcgtggc gatgaccggc gacggcgtca acgacgcgcc ggcgctgaag cgcgccgatg
    13981 tcggcgtggc gatgggtgcc aatggcaccg aggcggccaa ggaggccgcg gccgtcgtgc
    14041 tcgccgatga caacttcgcg acgattgccc gggcggtcga ggaaggccgc acgatctacg
    14101 acaacctgaa gaaggccatc gtgttctcgc tgccgaccaa cggcgcgcag gcctgcgtga
    14161 tcctcgccgc gatcgccttc ggcgtggcgc tgccggtgac gccggtgcag gtgctgtggg
    14221 tcaacatggt cgtggcggtg acgctgtcgc tgacgctggc cttcgagccc tccgagtccg
    14281 acgtcatgcg ccgtccgccg cgtgaccgcg acgcggcgct gatctcgggc ttcctcgcct
    14341 ggcgcgtggc gctggtctgc gtggtgcaga cgatcggctc gctcggcctg ttcctgtggg
    14401 agaccgcctc cggtgcgccg gtcgagcagg cgcgcacgct ggcggtcaac gcgctggtgg
    14461 tcggccagat cttctatctc ttcaacagcc gctacacggt cgcaccgtcg acctcgctcg
    14521 ccggcctgac cggcaaccgc gtcgcgctgc tcggcatcgc catcctgctg ggcctgcaga
    14581 tggccttcac ctacgtgccg ctgatgcaga cgctgctcgg caccgcggcg ctcggggcgc
    14641 gcgaatggct gctggcgctc ggcgtcggtg cgagcgtcta cgtcgtcgtc gagctggaga
    14701 aatgggcgct gcgcgcccgg ctcgcgcacc gcggcgccgg ctgacggggc tcaggggccg
    14761 gccggcggcg gcttcgcccc g
//
LOCUS       DAOQIE010000051        14443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2003_length_14443_cov_7.704893, whole genome shotgun sequence.
ACCESSION   DAOQIE010000051 DAOQIE010000000
VERSION     DAOQIE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2003_length_14443_cov_7.704893"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..465)
                     /locus_tag="PK144_05000"
     CDS             complement(1..465)
                     /locus_tag="PK144_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNF65120.1"
                     /translation="MKPLPKLMIDADACPVPVREICVRAARRLELELLLVANQPLPRP
                     KLARARVVTVTAGFDAADDWLVEAAGAGDVVVTADIPLAARLVAKAVHAINPRGTLYT
                     PANIGEALSLRDFMDGMRSAGLAQSRSAPYGERDRQQFASALDRVLTQALRG"
     gene            complement(471..1037)
                     /gene="ampD"
                     /locus_tag="PK144_05005"
     CDS             complement(471..1037)
                     /gene="ampD"
                     /locus_tag="PK144_05005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNF65121.1"
                     /translation="MTAESLRIGADGWCAAARRCPSPNHDARPEAMPVELIVIHGISL
                     PPGEFGGPWIDALFTNMLPADAHPYFRDIAALRVSAHFLIRRDGALVQYVPCGERAWH
                     AGASQWRGRTRCNDFSIGIELEGSDTTPYTDAQYTALAALIPALRRACPQIGADALAG
                     HCDIAPGRKTDPGPAFDWPRLHALLAAT"
     gene            complement(1034..1819)
                     /gene="murI"
                     /locus_tag="PK144_05010"
     CDS             complement(1034..1819)
                     /gene="murI"
                     /locus_tag="PK144_05010"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNF65122.1"
                     /translation="MFDSGLGGLSVLRELRAQLPHEDFLYVADSGHVPYGEKSDAFIR
                     ARTLAIAGFLLERRAKLLVVACNTATAAAVRELRAGCPVPVVGMEPAVKPAAAATRSG
                     TVGVLATTGTLASAKFAALLARFGRDVRVLTEPCPGWVDLVERGDCSGPAARAAVARH
                     VEPLLAQGADTLILGCTHYPFLKPLIAEVAGPGVALIDTGAAVARHTRAVLTAEGRLA
                     PGPRPGSEAFFTSGEPARLGPVLARLRGTAARLDALPAAVARA"
     gene            complement(1897..2904)
                     /locus_tag="PK144_05015"
     CDS             complement(1897..2904)
                     /locus_tag="PK144_05015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNF65123.1"
                     /translation="MQVGTTVTQFIIEEQRRHAHATGDFTSLINDIVIACKAIANAVS
                     RGSLAGVLGHAGTENVQGEDQKKLDVLANEAFLRCNEWGGRVAAMASEEMDDVYNLPA
                     VYPRGRYLLLFDPLDGSSNTDVNGIIGTIFSVLRCPDGVTEPSAEHFLQPGSQQVAAG
                     MAVYGPQTILVLTTGYGVNGFTLDDTIGEFVLTHPEMRVPEDSAEFAINASNQRHWEP
                     PVQRYIDECLAGTDGPRGRDFNMRWMGAMVGDVYRVLCRGGVFLYPADAKTAKKGGKL
                     RLMYEANPVAMLIEQAGGAASTGRERLLDLQPVGLHQRVPVIVGSKHEVERIVAYHRE
                     G"
     gene            3301..3624
                     /locus_tag="PK144_05020"
     CDS             3301..3624
                     /locus_tag="PK144_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65124.1"
                     /translation="MPLTSRNRRSIRSLGILTLVLFVVALTVSIGPAWLINPYIYDTA
                     QISFMSIPLWIATVGLALWVNQMTLDRRRPLDLLTLTLGRAPLLAIPIVLALLLVLTI
                     AGVRL"
     gene            complement(3629..5035)
                     /gene="dnaB"
                     /locus_tag="PK144_05025"
     CDS             complement(3629..5035)
                     /gene="dnaB"
                     /locus_tag="PK144_05025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNF65125.1"
                     /translation="MFLPYPSDAAAVADAGTEALRVPPHSLEAEQAVLGGLMLDNAAW
                     DTVADRVVEEDFYRGDHRLIFRAIRTLAEQGSPLDVVTLAEWLERSALLDDAGGLPYL
                     ATLARDTPSAANIRAYADIVRERSVLRQLIRVGTDIAGRAYQPEGRSSKELLDEAERE
                     VFAIAEQGSRQNQGFQPIKDLLTRAVDRIDTLFERDDPITGVPTGWSEFDEMTAGLQS
                     GELIIVAGRPSMGKTTFAMNIVEHAAIKENRPVAVFSMEMPADHLALRMMSSLGRIDQ
                     HRIRTGKLEDEDWPRLTSAVSLLAEAKLFIDDTAALSPTEVRARCRRLAREHGQLGLV
                     MLDYLQLMQVPGNSENRATEISEISRSLKALAKELHVPVIALSQLNRSLEQRPNKRPV
                     MSDLRESGAIEQDADLICFIYRDEVYHPDSADKGTAEIIIGKQRNGPIGSVRLTFLGK
                     YTRFENFMPDVYAADEYA"
     gene            complement(5236..5682)
                     /gene="rplI"
                     /locus_tag="PK144_05030"
     CDS             complement(5236..5682)
                     /gene="rplI"
                     /locus_tag="PK144_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNF65126.1"
                     /translation="MEVILLSKVENLGNLGDRVTVKSGYGRNFLIPRGKATEATPTNV
                     ARFEARRAELEAAAADMLAKAQARRDQLADLLVTMAAKAGAEGRLFGSIGPADIADVV
                     THAGYELKKSEVRMPEGPIRHVGEVEVDVHLHTDVNFKLHVNIIAE"
     gene            complement(5759..6664)
                     /locus_tag="PK144_05035"
     CDS             complement(5759..6664)
                     /locus_tag="PK144_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65127.1"
                     /translation="MTGIASRVMAGRLPAVLIVVATMFVSLLLPPVLLLSGAALALVT
                     LRRGPYEGALILGAASVAAFVLGWLLFGHPGPLAGVLPGFWLPVWGLAAVLRATVSLA
                     AAFAVAAGLGWLVVTGFYALLDDPADWWQGWLAELLKPAFEQAGAGGDAVQALDRLIA
                     WVAPLMPGMIAANVVFSALVALLIGRWWQARLYNPGGFRSEFHGLRLGRGLALLTIVT
                     GAAAFASGALWLQCLALPLAVVWLLQGLAVLHGLALLRGWSPWALVLVYVLLLLLLPQ
                     AVALLCLLGLIDTWMNLRARAAPPG"
     gene            complement(6735..6959)
                     /gene="rpsR"
                     /locus_tag="PK144_05040"
     CDS             complement(6735..6959)
                     /gene="rpsR"
                     /locus_tag="PK144_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNF65128.1"
                     /translation="MSRFFRRRKYCRFTAEGVTEIDYKDLGTLKNYITETGKIVPSRI
                     TGTKARYQRQLATAIKRARFLALLPYTDRH"
     gene            complement(6996..7385)
                     /gene="rpsF"
                     /locus_tag="PK144_05045"
     CDS             complement(6996..7385)
                     /gene="rpsF"
                     /locus_tag="PK144_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNF65129.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIDRYRSMIETDGGAIHRLEDWGR
                     RQLAYPINKVHKAHYVLMNVECSQAVLDEIESAFRFNDAVLRNLIISRPGPDTEASPL
                     AKSREGREERESRHDDVAAEVDASDDE"
     gene            complement(7514..8263)
                     /gene="rlmB"
                     /locus_tag="PK144_05050"
     CDS             complement(7514..8263)
                     /gene="rlmB"
                     /locus_tag="PK144_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017764011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNF65130.1"
                     /translation="MSEELIYGLHAVGAALRHEPQRLRGLWVDRRRRDARIKELLALA
                     EAAGVRVYAEDAEALDRQCGGERHQGVFARMKVAERQWREDELDALLDAIEGPALLLV
                     LDGVQDPHNLGACLRSADAAGVHAVLAPNDRAAGLTPTARKVACGAAEHVPFIPVTNL
                     ARTLRELKQRGIWLVGAAGEAEADLYAMDLAGPVALVLGAEEKGLRRLTREECDYLAH
                     IPMTGTVESLNVSVATGVFLFEALRQRRARR"
     gene            complement(8332..10659)
                     /gene="rnr"
                     /locus_tag="PK144_05055"
     CDS             complement(8332..10659)
                     /gene="rnr"
                     /locus_tag="PK144_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNF65131.1"
                     /translation="MTARSRRPWHRLDPFFAREQEKYGRAVPSREFILQTLEAAPEPL
                     TLERLCADWGIHAQDETEPLLRRLQAMTRDGQIVCNRRGGYGPVAKMDLVRGRIIGHP
                     EGFGFLVPDEGGEDLFVPPHEMRQLLHGDRVLMCVTGIDRRGRREGSVVEVLERGTTR
                     LVGRYVEDNGIGFVLPDNRRLGAHDFLVPPAEKGGARPGQIVVAELIQQPSKQAMPIA
                     RVREVLGDHMAPGMEVQIAIVNHGIPHEFPPEVVAEAEAFGPSVPASALEGRLDLREQ
                     PFVTIDGLTARDFDDAVYCEKKRGGGFRLFVAIADVAHYVRPGTPLDAEAQARGTSVY
                     FPDRCIPMLPEALSNGLCSLNPHVERLVMVCELNIGADGEFGRARFHEGVIRSHARLT
                     YDAVAAMLVDRDEALRAEFAALVPHLERLYALYGVLRAARERRGAIDFETQETEIEYG
                     PERKIERIVPSQRNDAHKLIEECMIAANVATAQHLEAKKIPALFRIHEGPRLAKLEKL
                     RGFLKGLGLYLGGGDTPGSEDYARLFEAIQNRPDFALIQTVMLRSLAQAVYSPDNAGH
                     FGLALAAYAHFTSPIRRYPDLLVHRALKHLIHHGGKARGYGYSHEKMTELGEHCSMTE
                     RRADDATRDAVEWLKCEYMRDKVGETFDGTVSAVTAFGLFVLLKEIFVEGLVHISNLP
                     GDYYHFDPLAHRLEGERFGRCYRLGDALRVQVARVDLDERKIDFMPADVAGVGIGLPP
                     TPRKPAGRPSRAARDDGGKPPARGGAGGPYRKRRR"
     gene            10839..10923
                     /locus_tag="PK144_05060"
     tRNA            10839..10923
                     /locus_tag="PK144_05060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10873..10875,aa:Leu,seq:cag)
     gene            10940..11024
                     /locus_tag="PK144_05065"
     tRNA            10940..11024
                     /locus_tag="PK144_05065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10974..10976,aa:Leu,seq:cag)
     gene            complement(11171..11959)
                     /locus_tag="PK144_05070"
     CDS             complement(11171..11959)
                     /locus_tag="PK144_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNF65132.1"
                     /translation="MLLSALYRYPVKSCRGARLDAAMLDRYGIAGDRHWMLVEAASGR
                     FVTQRQHARMALIDTQWHGDTLGLSVAGAGGVEVTSGIGEPRTVEVWGEVVQALDAGD
                     EAAAWLSEYLGLAVRLVECGPAHRRPVDPAYDRIGAETGFSDGFPLLLISEASLADLN
                     SRMERPVEMIRFRPNLVVSGCAPFAEDGWKRLRIGGIELHVVKPCSRCPIPTIDPATA
                     ARGREPMATLLKYRREGQKTYFGQNLIHAGPGELQAGMRVEVLE"
     gene            complement(12041..12991)
                     /locus_tag="PK144_05075"
     CDS             complement(12041..12991)
                     /locus_tag="PK144_05075"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNF65133.1"
                     /translation="MEGLADALLRAALTSVGGIDGCVTEFVRVTDTLLPRAVWLRRVP
                     ELAHGGRTLAGVPVVVQLLGSDPQCLADNAARVAGLGAPGVDLNFGCPAPTVNRHRGG
                     AALLDEPELLARIVAACRAALPPAVPLTAKMRLGIRDAARALDCARALADGGAAEITV
                     HARTKLDGYRPPAHWDWIARIREAVAVNVIANGEVWSPADAARCLAVSGCRDLMLGRG
                     LVTRPGLALNIRSQRAGHEPLPLPWPRLLALLDTFYAGVRAQLLPRHAPGRLKQWLSY
                     LRNAYPEAQALFARVRTETDAARLGEALAAAHAHPVPLPP"
     gene            complement(13030..13425)
                     /gene="gloA"
                     /locus_tag="PK144_05080"
     CDS             complement(13030..13425)
                     /gene="gloA"
                     /locus_tag="PK144_05080"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNF65134.1"
                     /translation="MRYLHTMIRTGDLDRSIAFYRDVLGFRLVSRSDYPDGEFTLAFL
                     QAPGDDGNDGPMLELTYNWGVSSYEPGKGYGHVAFKVESMDAFAENLRARGHDFSWGP
                     GKAPNGRTAIAFIVDPDGYQIELIERADA"
     gene            complement(13470..13787)
                     /locus_tag="PK144_05085"
     CDS             complement(13470..13787)
                     /locus_tag="PK144_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HNF65135.1"
                     /translation="MAQFDVCRNPNAGTATRVPYLLDVQHDLIAVFESRAIVPLVDPA
                     LVREKLVQRRLPVFTIIDRRLVRMPQSITGRPAHTPGPAVASLAGQRAELLAALELLF
                     TGI"
     gene            complement(13797..14042)
                     /locus_tag="PK144_05090"
     CDS             complement(13797..14042)
                     /locus_tag="PK144_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HNF65136.1"
                     /translation="MAAVFDPDVPRTATGLGVNTGLLRQVREFGVPLDDSCERALAEA
                     VRAARMRAWQEENRPWIEAYNAWIAEHGCFGEEYRSF"
     gene            complement(14111..>14443)
                     /locus_tag="PK144_05095"
     CDS             complement(14111..>14443)
                     /locus_tag="PK144_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNF65137.1"
                     /translation="SLCLDREVAHLKDELMPRYASIIYNGYWWSPERRMLQAMIDASQ
                     APVNGVVRLKLYKGNVMVAGRKSDDSLFDPTIATFEDDGGAYNQKDAEGFIKLNALRM
                     RIAANKGR"
BASE COUNT         2199 a         5058 c         5066 g         2120 t
ORIGIN      
        1 tcagccgcgc agcgcctgcg tcagcacgcg atcgagggcc gaggcgaact gctggcggtc
       61 gcgctcgccg tacggggcgc tgcgcgactg cgcgagcccg gcgctgcgca tgccgtccat
      121 gaagtcgcgc agcgacagcg cctcgccgat gttggccggc gtgtagagcg tgccgcgcgg
      181 gttgatcgca tgcacggcct tcgcgaccag ccgcgccgcg agcggaatgt cggcggtgac
      241 gaccacgtcg ccggccccgg ccgcttccac cagccagtcg tcggcggcat cgaagccggc
      301 ggtgaccgtc acgacgcgcg cccgcgcgag cttcgggcgc ggcagcggct gattggcgac
      361 cagcagcagt tcgagttcga gccgccgcgc ggcgcgcacg cagatctcgc gcaccggcac
      421 cggacaggca tcggcgtcga tcatcagctt cggcagcggc ttcatgttgt tcatgttgcc
      481 gccagcagcg catgcaggcg cggccagtcg aaggccggcc ccggatcggt cttgcggccc
      541 ggcgcgatgt cgcagtgacc ggcgagcgca tcggcgccga tctgcggaca ggcgcggcgc
      601 agcgccggga tcagcgctgc cagcgccgtg tactgggcat cggtgtacgg cgtcgtgtcg
      661 ctgccttcga gctcgatgcc gatcgagaaa tcattgcagc gcgtgcgccc gcgccactgc
      721 gaggccccgg cgtgccaggc gcgttcgccg cagggcacgt actgcacgag tgcgccgtcg
      781 cgccggatca ggaaatgcgc cgacacccgc agcgccgcga tgtcgcggaa atacgggtgg
      841 gcatcggccg gcagcatgtt cgtgaacagc gcatcgatcc acggcccgcc gaactcgccc
      901 ggcggcagcg agatgccgtg aatgacgatc agctcgaccg gcattgcctc cgggcgcgca
      961 tcgtgattcg gcgaaggaca gcggcgcgcg gccgcacacc agccatcggc cccgatgcgc
     1021 aggctttccg ccgtcatgcc ctcgccaccg ccgccggcag cgcatcgagc cgcgccgcag
     1081 tcccccggag ccgcgccagc accggtccga gccgggccgg ttcgccgctg gtgaagaagg
     1141 cttcgctacc cggccgcggc cccggcgcca gccggccttc ggccgtcagc acggcgcgcg
     1201 tgtgccgggc gaccgccgcg ccggtatcga tcagcgccac gccgggaccg gcgacctcgg
     1261 caatcagcgg tttcaggaac gggtaatgcg tgcagccgag gatcagcgtg tcggcgccct
     1321 gcgcgagcag cggctcgacg tggcgggcga ccgccgcgcg cgcggccggg ccgctgcagt
     1381 cgccgcgctc gaccagatcg acccagcccg ggcagggctc ggtcagcacg cgcacgtcgc
     1441 gcccgaagcg cgccagcagc gccgcgaact tcgcgctcgc cagcgtgccg gtggtcgcga
     1501 gcacgccgac cgtgccgctg cgcgtcgccg ccgccgccgg cttcacggcc ggctccatgc
     1561 cgaccaccgg caccgggcag ccggcgcgca gctcgcgcac ggccgcagcg gtcgcagtgt
     1621 tgcaggcgac gaccagcagc ttcgcgcgcc gttcgagcag gaagccggcg atcgcgagcg
     1681 tgcgggcgcg gatgaaggcg tcggacttct cgccgtacgg gacgtggccg gaatcggcga
     1741 cgtacaggaa atcctcatgc ggcagctgcg cgcgcagctc gcgcagcacc gacaggccgc
     1801 cgaggccgga atcgaacacg ccgaccggcg cggctgcctc gccggtgccc ggcggcggcg
     1861 gcgccccggg cgcaagtgtc gtgccgtcca gaatgctcag ccttcgcggt gataggcgac
     1921 gatgcgctcg acttcgtgct tcgaaccgac gatgaccggc acgcgctggt gcaggccgac
     1981 cggctgcagg tcgagcaggc gctcgcgacc ggtgctcgcc gcaccgccgg cctgctcgat
     2041 cagcatcgcc accggattgg cctcgtacat cagccgcagc ttgccgccct tcttcgcggt
     2101 cttggcgtcg gccgggtaca ggaacacgcc gccgcggcag agcacgcggt agacatcgcc
     2161 gaccatcgcc cccatccagc gcatgttgaa gtcgcgcccg cgcgggccgt ccgtgccggc
     2221 cagacattcg tcgatgtagc gctgcaccgg cggctcccag tggcgctggt tcgaggcgtt
     2281 gatcgcgaac tcggcgctgt cctcgggcac gcgcatctcg ggatgcgtca gcacgaactc
     2341 gccgatcgtg tcatcgagcg tgaagccgtt gacgccgtag ccggtggtca gcacgaggat
     2401 cgtctgcgga ccatagaccg ccatgccggc ggcgacctgc tgcgagccgg gctgcaggaa
     2461 gtgctcggcg ctcggctcgg tgacgccgtc cgggcagcgc agcaccgaga agatcgtgcc
     2521 gatgatgccg ttcacgtcgg tgttcgagga gccgtcgagc ggatcgaaca gcagcaggta
     2581 gcggccgcgc ggatacacgg ccggcaggtt gtagacgtcg tccatttcct ccgacgccat
     2641 cgccgccacg cgcccgcccc attcgttgca gcgcaggaag gcttcgttgg cgagcacgtc
     2701 gagcttcttc tggtcctcgc cctgcacgtt ctcggtgccg gcgtggccga gcacgccggc
     2761 gagcgatccg cggctgacgg cgttcgcgat cgccttgcag gcgatgacga tatcgttgat
     2821 cagcgaggtg aaatcgccgg tcgcatgggc atgccggcgc tgctcctcga tgatgaactg
     2881 cgtgacggtg gttccgactt gcatggggga ttccttcgac acggtgccgc tgccggaaga
     2941 gttccggtcc ggagaccagg agcacggtat gcgacgcggg caccctgccc ccgggccgac
     3001 ggcgggactg aaaactttac cacaaggtct gcgccccgcc gcccgggacc accgggatgc
     3061 gacacggcct ggaaccggcg ccgggacacg gaccgccggc atggcccgga tgcgcgatcg
     3121 ccggccatcg ttgcgatttt gtgccgcttc attgcgcgaa cgtcacgttg tgccgcaaac
     3181 cgtgacttcc cgccgccggc gttctgtccc acactcgccg catgacctcc ggcagggagc
     3241 ccggaactgc atctggcacg accccccgtg cccaccgctg ccgcatctgg taccgccgcc
     3301 atgcccctca cttcacgcaa ccgacgctcg atccgctcgc tcggcatcct gaccctcgtg
     3361 ctgttcgtcg tcgccctgac cgtctcgatc gggccggcct ggctgatcaa cccgtacatc
     3421 tacgacacgg cgcagatctc gttcatgagc atcccgctgt ggatcgcgac cgtcgggctg
     3481 gcgctgtggg tcaaccagat gacgctggac cgccgccggc cgctcgacct gctgacgctg
     3541 acgctcgggc gggcgccgct gctggcgatt ccgatcgtgc tggcactgct gctggtgctg
     3601 acgatcgccg gcgtgcggct ctgaggcttc aggcgtactc gtccgccgcg tacacgtcgg
     3661 gcatgaagtt ctcgaagcgc gtgtacttgc cgaggaaggt caggcgcacc gaaccgatcg
     3721 gaccgttgcg ctgcttgccg atgatgatct cggccgtgcc cttgtcggca ctgtccgggt
     3781 gatagacctc gtcgcgatag atgaagcaga tcaggtcggc atcctgctcg atcgcgcccg
     3841 attcgcgcag atccgacatc accggccgct tgttcggccg ctgctccagc gagcggttga
     3901 gctgtgacag cgcgatcacc ggcacgtgca gctccttggc gagcgccttc agcgagcgcg
     3961 agatttccga gatctcggtc gcacggttct cggagtttcc cggcacctgc atcagttgca
     4021 ggtaatcgag catcaccagc ccgagctggc cgtgctcgcg cgccagacgc cggcagcggg
     4081 cgcggacttc ggtcgggctc aacgccgccg tgtcgtcgat gaacagcttg gcctcggcga
     4141 gcaggctgac ggccgaggtc agccgcggcc agtcctcgtc ctccagcttg ccggtgcgga
     4201 tgcggtgctg gtcgatgcgc ccgagcgagg acatcatgcg cagcgccagg tgatcggccg
     4261 gcatttccat cgagaacacc gcgaccgggc ggttctcctt gatcgccgcg tgctcgacga
     4321 tgttcatcgc gaaggtcgtc ttgcccatcg acgggcggcc ggccacgatg atcagctcgc
     4381 cggactgcag gccggcggtc atctcgtcga attcgctcca gccggtcggc acgccggtga
     4441 tcgggtcgtc gcgctcgaac agcgtgtcga tgcgatcgac cgccctggtc agcagatcct
     4501 tgatcggctg gaaaccctgg ttctggcggc tgccctgctc ggcgatcgcg aacacctcgc
     4561 gctcggcctc gtcgagcagt tccttgctgc tgcgcccctc gggctgatag gcgcgcccgg
     4621 cgatgtcggt gccgacgcgg atcagctgcc ggagcaccga acgctcacgc acgatgtcgg
     4681 cataggcacg gatgttggcc gcgctcggcg tgtcacgtgc gagcgtcgcg agatacggca
     4741 ggccgccggc atcgtcgagc agcgcgctgc gctccagcca ttcggcgagc gtgaccacgt
     4801 cgagcggcga gccctgctcg gcgagcgtgc ggatggcgcg gaagatcagc cggtgatcgc
     4861 cgcggtagaa atcctcctcg accacgcgat cggcgaccgt gtcccaggcg gcgttgtcga
     4921 gcatcaggcc gccgagcacg gcctgctcgg cctccagcga atgcggcggc acgcgcaagg
     4981 cctcggtgcc ggcatcggca acggcggcag cgtcggacgg atacggcagg aacactcggc
     5041 acgacctcgc aagtcaggca ggcagcggat cggcccggcg cgaccgggcg gctcgcgaat
     5101 ctagcacagg cctgcgccgg cggtgccgca gacgacgaac ggccccggcc accggcgtcg
     5161 caggacaccg gcggccgggg ccgcgtgcgg gaggacttcg gcgacgactg cgtcggagcc
     5221 ggtcctgccc ggtgattact cggcgatgat gttgacgtgc agcttgaagt tcacgtcggt
     5281 gtgcaggtgc acgtcgacct cgacctcgcc gacgtggcgg atcgggcctt ccggcatgcg
     5341 cacttcggac ttcttcagct cgtagccggc gtgggtgacg acatcggcga tgtcggccgg
     5401 gccgatcgag ccgaacagcc ggccttcggc accggccttg gcggccatgg tcaccagcag
     5461 gtcggcgagc tggtcgcggc gggcctgggc cttggcgagc atgtcggccg cagcggcctc
     5521 cagctcggcc cggcgggcct cgaagcgggc gacgttggtc ggcgtggcct cggtggcctt
     5581 gccgcgcggg atcaggaaat tgcggccgta accggacttg acggtgacgc ggtcaccgag
     5641 gttgccgagg ttttcgacct tcgagagcag gatgacttcc atcgtcggaa ctccgaatgc
     5701 gtggacagtg cgtgaatgta cggttgggcg gatcaggcga ttcgttcggt acgggcattc
     5761 atcccggggg cgcggcacgg gcgcgcaggt tcatccaggt atcgatcagt ccgagcaggc
     5821 agagcagcgc gacggcctgc ggcagcaaca gcagcagcag cacgtacacg agcaccagcg
     5881 cccacggcga ccagccgcgc agcagcgcca gcccatgcag caccgcgagc ccctgcagca
     5941 gccagaccac cgccagcggc agtgccaggc actgcagcca gagcgcgccg ctcgcgaagg
     6001 ccgccgcgcc ggtgacgatc gtcagcagcg ccagcccgcg cccgaggcgc aggccgtgga
     6061 actcgctgcg gaaaccgccc gggttgtaca gccgcgcctg ccaccagcgc ccgatcagca
     6121 gcgccaccag cgccgagaac acgacgttgg ccgcgatcat ccccggcatc agcggtgcga
     6181 cccaggcgat cagccggtcg agcgcctgca ccgcatcacc gccggcaccg gcctgttcga
     6241 aggccggctt cagcagttcg gcgagccagc cctgccacca gtcggccgga tcgtcgagca
     6301 gcgcataaaa gccggtgacc accagccagc cgaggccggc cgcgaccgcg aacgccgccg
     6361 cgagcgacac cgtcgcacgc agcaccgccg ccagtcccca gaccggcagc cagaagcccg
     6421 gcagcacgcc ggcgagcggc cccggatggc cgaacagcag ccagccgagc acgaaagccg
     6481 cgacgctggc ggcgccgagg atcagcgcac cctcgtacgg cccgcgcctg agcgtcacca
     6541 gcgccagcgc cgcgcccgag agcagcagca ccggcggcag cagcagcgat acgaacatcg
     6601 tcgcgacgac gatcagcacg gccggcagcc gtcctgccat cacccgcgac gcgatacctg
     6661 tcatgaatga acccgcccgc aggccgcgac cgcccggaac cggatcaacc cggtaccggc
     6721 gttcgcagcg ccgctcagtg acggtcggtg tagggcagca gcgcgaggaa acgcgcgcgc
     6781 ttgatcgccg tggcgagctg gcgctggtag cgcgccttcg tgccggtgat gcggctcggc
     6841 acgatcttgc ccgtctcggt gatgtagttc ttcagcgtgc cgagatcctt gtaatcgatc
     6901 tcggtgacgc cttcggcggt gaagcggcag tacttgcggc gacggaagaa gcgggacatg
     6961 gcggattcga tcctggtgcg tggaaggcgg acggcttatt cgtcgtcgga ggcgtcgact
     7021 tcggcggcga cgtcgtcgtg acgcgactcg cgctcctcgc ggccctcgcg ggacttggcg
     7081 agcggcgaag cctcggtgtc cggacccgga cggctgatga tcaggttgcg cagcaccgca
     7141 tcattgaagc ggaacgcgct ctcgatctcg tcgagcacgg cctggctgca ctcgacgttc
     7201 atcagcacat agtgcgcctt gtgcaccttg ttgatcggat aggccagctg gcggcggccc
     7261 cagtcctcga gacggtggat cgcaccgccg tcggtctcga tcatcgaccg gtaacgatcg
     7321 atcatcgccg gcacctgctc gctctggtcc gggtggacca ggaagacgat ttcgtagtga
     7381 cgcatcgaag ctccttacgg atgaaaaaca gccgcccggc ggtccggtgc ggcaaggagt
     7441 cggccctgtc ggaccggaaa agccgcgcag tgtatggagt tgtccgcgcc cgggcaagcc
     7501 gcggcgccgg ctttcagcgc cgcgcgcggc gctggcgcag cgcctcgaac aggaacacgc
     7561 cggtcgcgac cgagacgttc aggctctcga ccgtgccggt catcgggatg tgcgccaggt
     7621 agtcacattc ctcgcgcgtc agccggcgca ggcctttttc ctcggccccg agcaccagcg
     7681 ccaccggccc ggcgagatcc atcgcataca gatcggcctc ggcctcgccg gcggcgccga
     7741 ccagccagat gccgcgctgc ttcagctcgc gcagcgtgcg cgcgaggttg gtgaccggga
     7801 tgaacggcac gtgctcggcc gcgccgcagg ccaccttgcg cgcggtcggc gtcaggccgg
     7861 cggcgcggtc gttcggggcg agtaccgcgt gcacgccggc ggcgtcggcc gagcgcaggc
     7921 aggcgccgag gttgtgcggg tcctgcacgc cgtcgagcac cagcagcagc gccggcccct
     7981 cgatggcatc gagcagcgca tcgagttcgt cctcgcgcca ctggcgttcg gcgaccttca
     8041 tgcgcgcgaa cacgccctgg tggcgctcgc caccgcactg gcgatcgagc gcttcggcgt
     8101 cctcggcgta gacccgcacg ccggcggcct cggccagcgc cagcagttcc ttgatgcgcg
     8161 catcgcgccg gcgccggtcc acccacaggc cgcgcagccg ctgcggctcg tgacgcagcg
     8221 ccgcaccgac cgcatgcagg ccgtagatca gttcctcgct catgtcggca ggactccggg
     8281 ccgcgtgcca acggcacgca gcggttcatc gttcagggat cgggtccggc ttcagcgccg
     8341 gcgcttgcgg tacggcccgc ccgcgccccc gcgcgccggc ggcttgccgc cgtcatcccg
     8401 ggcggcacgc gacgggcgcc ccgccggctt gcgcggcgtc ggcggcaggc cgatgccgac
     8461 gccggcgaca tcggccggca tgaaatcgat cttgcgctcg tcgagatcga cgcgcgccac
     8521 ctgcacgcgc agcgcatcgc cgagccggta gcagcgtccg aagcgctcgc cctccagccg
     8581 gtgggccagc ggatcgaagt ggtaatagtc gccgggcagg ttggagatgt gcacgagccc
     8641 ctcgacgaag atctccttca gcagcacgaa cagcccgaag gccgtgaccg ccgacaccgt
     8701 gccgtcgaag gtctcgccga ccttgtcgcg catgtattcg cacttcagcc attcgacggc
     8761 atcgcgggtc gcgtcgtcgg cgcgccgctc ggtcatcgag cagtgctcgc cgagttcggt
     8821 catcttctcg tgcgagtagc cgtagccccg ggccttgccg ccgtggtgga tcaggtgctt
     8881 cagcgcgcga tgcacgagca ggtccggata gcggcggatc ggcgacgtga agtgcgcata
     8941 ggccgcgagc gcgaggccga agtgaccggc gttgtcgggg ctgtacacgg cctgcgccag
     9001 cgagcgcagc atgacggtct ggatcagcgc gaagtccggc cggttctgga tggcctcgaa
     9061 cagccgggcg taatcctcgg agcccggcgt gtcgccgccg ccgagataca gcccgagccc
     9121 cttcaggaag ccgcgcagct tttcgagctt cgcaagccgc ggaccttcgt ggatgcggaa
     9181 cagcgccggg atcttcttcg cttcgaggtg ctgcgcggtc gcgacattgg ccgcgatcat
     9241 gcattcctcg atcagcttgt gcgcatcgtt gcgctgcgag ggcacgatgc gctcgatctt
     9301 gcgctccggc ccgtattcga tctcggtttc ctgggtttcg aagtcgatcg ccccgcggcg
     9361 ctcgcgcgcc gcgcgcagca cgccgtacag cgcgtacaga cgttcgagat gcggcacgag
     9421 ggcggcgaac tccgcgcgca aggcctcgtc gcgatcgacc agcatcgcgg ccaccgcgtc
     9481 gtaggtcagc cgtgcgtgcg agcggatcac gccttcgtga aagcgcgcgc ggccgaactc
     9541 gccgtcggcg ccgatgttca gctcgcagac catcaccagc cgctcgacgt gcgggttcag
     9601 cgagcagagc ccgttcgaca gcgcctcggg cagcatcggg atgcagcggt ccgggaagta
     9661 caccgaggtg ccgcgcgcct gcgcctcggc atcgagcggc gtgccggggc gcacgtaatg
     9721 cgcaacgtcg gcaatggcga cgaacagccg gaagccgccg ccgcgcttct tctcgcagta
     9781 gacggcgtcg tcgaagtcgc gggcggtgag accgtcgatg gtcacgaacg gctgctcgcg
     9841 caggtcgagc cggccttcga gcgccgatgc gggcacgctc gggccgaagg cttcggcctc
     9901 ggcgacgacc tcgggcggga actcgtgcgg aatgccgtga ttgacgatgg cgatctgcac
     9961 ttccatgccc ggcgccatgt gatcgccgag cacctcgcgc acgcgcgcga tcggcatcgc
    10021 ctgcttcgag ggctgctgga tcagctcggc gacgacgatc tgcccggggc gcgcaccgcc
    10081 cttctcggcc ggcggcacca ggaagtcatg cgcgccgagc cggcggttgt cgggcagcac
    10141 gaaaccgatg ccgttgtcct cgacgtagcg cccgaccagc cgcgtcgtgc cgcgttcgag
    10201 gacctcgacc accgagccct cgcgccggcc gcggcggtcg atgccggtca cgcacatcag
    10261 cacgcggtcg ccgtgcagca gctggcgcat ctcgtgcggc ggcacgaaca gatcctcgcc
    10321 gccctcgtcc ggcaccagga agccgaagcc ctcggggtga ccgatgatgc gcccgcgcac
    10381 cagatccatc ttcgcgaccg gcccgtagcc cccgcggcgg ttgcagacga tctggccgtc
    10441 gcgcgtcatc gcctgcaggc ggcgcagcag cggttcggtt tcgtcctggg cgtggatgcc
    10501 ccagtcggcg cacagccgtt cgagcgtcag cggctcgggc gcggcttcga gcgtctgcag
    10561 gatgaattcg cggctcggta cggcgcggcc gtatttttcc tgctcgcgcg cgaagaacgg
    10621 gtcgagccgg tgccagggac gtcgggaacg ggcagtcatc gggaatgcgg aaacctcgcg
    10681 aaaaatgaag gaatgacacg ataacaccgc cccgtccgga gcatgcacaa aacgtgcgga
    10741 gggcgtttga cagcccccgg ggccgtgcgt atagtgcgcc ccacttcgcg accccctccc
    10801 ctgcggaccg ggatcgtggc gtgaaatgca gcagccttgc cgaagtggcg gaactggtag
    10861 acgcgctagt ttcaggtact agtggggcga cccgtggagg ttcgagtcct ctcttcggca
    10921 ccacgcgctg cagcaagatg cccaggtggc ggaactggta gacgcgctag tttcaggtac
    10981 tagtagggag acctgtggag gttcgagtcc tctcctgggc accactccat catgatcatg
    11041 atgatgagtg tctctcccga gaaaacggcc ccgaaagggg ctttttcttt tccggcttcc
    11101 ggcgaacgtc cgcgtgctgt tccggcaccg cccgctggcc gctgcaacga ccagcggcgg
    11161 gcgcgcatgg ctattccagc acctccacgc gcatgccggc ctgcaattcc cccggaccgg
    11221 catggatgag gttctggccg aagtaggtct tctgcccttc gcggcggtac ttcagcagcg
    11281 ttgccatcgg ttcgcgcccg cgtgcggccg ttgccggatc gatggtcgga atcgggcagc
    11341 gcgagcacgg cttgaccacg tgcagctcga tgccgccgat gcgcagccgc ttccagccat
    11401 cctcggcgaa gggcgcgcag ccgctgacca ccagattcgg ccggaagcgg atcatctcga
    11461 ccgggcgctc catgcggctg ttgaggtcgg ccagcgaagc ctccgagatc agcagcagcg
    11521 ggaagccatc ggaaaagccg gtctcggcgc cgatgcggtc ataggccgga tcgaccggcc
    11581 gccggtgcgc ggggccgcat tcgaccaggc gcacggcgag cccgaggtac tccgacagcc
    11641 aggccgcggc ctcgtcgccg gcatcgagcg cctgcacgac ctcgccccag acctcgaccg
    11701 tgcgcggctc gccgatgccg ctggtgacct cgacgccacc ggcaccggcc accgacaggc
    11761 cgagcgtgtc gccatgccac tgcgtgtcga tcagcgccat gcgcgcgtgc tggcgctgcg
    11821 tgacgaagcg gccgctcgcg gcctcgacca gcatccagtg ccggtcgccg gcgatgccgt
    11881 agcgatcgag catcgcggca tcgaggcgcg cgccgcggca ggacttcacg gggtagcggt
    11941 acagcgcgct cagcagcacg tcggtcatcg ggcagcctgc ctgtgggtgg tccgggaaaa
    12001 acatccggcg cgactgtagc gaatgccgcc ggcacgcgcc tcagggcggg agcggtacgg
    12061 gatgggcgtg cgcggcggcc agcgcctcgc caagtcttgc ggcatcggtt tcggtgcgca
    12121 cgcgcgcaaa cagcgcctgt gcttccggat aggcgttgcg cagataggac agccactgct
    12181 tcagccgccc cggcgcgtgc cgcggcagca gctgcgcacg cacgccggca tagaaggtat
    12241 cgagcagcgc cagcagccgc ggccagggca gcggcagcgg ttcgtggccg gcgcgctggc
    12301 tgcggatgtt cagcgccagc cccggacgcg tcaccagccc gcgcccgagc atcaggtcgc
    12361 ggcagccgga aacggcgagg caccgggcgg catcggccgg gctccagacc tcgccgttgg
    12421 cgatgacgtt gacggcgacc gcctcgcgga tgcgggcgat ccagtcccag tgtgccggcg
    12481 gccggtagcc gtcgagcttc gtgcgggcat gcacggtgat ttcggccgcc ccgccgtcgg
    12541 cgagcgcccg tgcgcagtcg agggcgcgcg cggcatcgcg gatgccgagg cgcatcttgg
    12601 cggtcagcgg caccgccggc ggcagcgccg cgcggcaggc ggcgacgatg cgcgcgagca
    12661 gttccggctc atcgagcagc gccgcgccgc cgcggtgacg gttcacggtc ggggccgggc
    12721 agccgaagtt cagatccacg cccggcgcgc cgagcccggc gacgcgcgcg gcgttgtcgg
    12781 cgaggcactg cggatcggag ccgagcagct gcacgaccac cggcacgccc gcgagcgtgc
    12841 gcccgccgtg cgcgagttcc ggcacgcgcc gcaaccacac cgcgcgcggc agcagcgtgt
    12901 cggtgacgcg cacgaactcg gtcacgcagc cgtcgatgcc gccgaccgaa gtcagcgcgg
    12961 cgcgcagcag cgcatcggcg agcccctcca tcggcgccag cagcagccgg ccaccggcgg
    13021 cgacggccgt caagcgtcgg cgcgctcgat cagctcgatc tggtagccgt ccggatcgac
    13081 gatgaaggcg atcgcggtgc ggccgttcgg ggccttgccc gggccccagc tgaagtcgtg
    13141 gccgcgggca cgcaggtttt cagcgaaggc gtccatcgac tcgaccttga acgcgacgtg
    13201 gccgtagccc ttgccgggtt cgtaggacga cacgccccag ttgtaggtca gctccagcat
    13261 cggcccatcg ttgccgtcat cgcccggggc ctggaggaag gcgagcgtga attcgccgtc
    13321 cgggtaatcg ctgcgcgaca ccagccggaa gccgagcacg tcacggtaga aggcgatcga
    13381 gcggtcgaga tcgccggtgc ggatcatcgt gtgcaggtag cgcatggcag atgccctcgg
    13441 gggtacgccg gtcaggggaa ggaccggatt cagatgccgg tgaacagcag ctcgagtgcg
    13501 gcgagcagtt ccgcgcgctg gccggccagc gaggcgacgg cgggaccggg cgtgtgcgcc
    13561 ggccggccgg tgatcgattg cggcatccgc accagtcgtc gatcgatgat cgtgaagacc
    13621 ggcagccgcc gctgcacgag cttctcgcgc acgagcgccg gatcgaccag cggcacgatg
    13681 gcgcgcgact cgaagacggc gatcagatcg tgctgcacgt cgaggaggta cggcacccgc
    13741 gtggccgtgc cggcattcgg attgcggcag acatcgaact gcgccatcgc cgtgactcag
    13801 aagctccggt attcctcgcc gaaacagcca tgctcggcaa tccaggcgtt gtatgcctcg
    13861 atccacggac ggttctcttc ctgccacgcc cgcatgcgcg ccgcgcgcac ggcctcggcc
    13921 agtgcgcgtt cgcagctatc gtcgagcggg acgccgaact cgcggacctg gcgcagcagg
    13981 ccggtgttga cgcccagtcc ggtcgcggta cgcgggacat cggggtcgaa gacggcggcc
    14041 atcgggggat tcctcggcgc aggcaggaga ttctgccggc gagtatgacc cgaaagccgc
    14101 cgggcgcgac tcagcggccc ttgttggcag cgatgcgcat gcgcagggcg ttgagcttga
    14161 tgaagccctc ggcgtccttc tggttgtagg cgccgccgtc gtcctcgaag gtcgcgatgg
    14221 tcgggtcgaa cagcgaatcg tccgacttgc gaccggcgac catgacgttg cccttgtaga
    14281 gcttcaggcg cacgacgccg ttcaccgggg cctgcgaggc atcgatcatg gcctgcagca
    14341 tccgccgctc cggcgaccac cagtagccgt tgtagatgat gctcgcgtag cgcggcatca
    14401 gctcgtcctt caggtgcgcg acctcgcgat cgaggcacag cga
//
LOCUS       DAOQIE010000052        14237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2042_length_14237_cov_7.097095, whole genome shotgun sequence.
ACCESSION   DAOQIE010000052 DAOQIE010000000
VERSION     DAOQIE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2042_length_14237_cov_7.097095"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK144_05100"
     CDS             <1..345
                     /locus_tag="PK144_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNF65138.1"
                     /translation="VVAYSDAHVHAMSAALLKPGDAVLAISQSGRSPELLGSAQRARA
                     AGADLVAITVAGSPLAALATVAITVPAVAAAPGPLPGGSRVADLAIVDALAIGVALRR
                     TPLTPESPGLET"
     gene            351..2009
                     /gene="pgi"
                     /locus_tag="PK144_05105"
     CDS             351..2009
                     /gene="pgi"
                     /locus_tag="PK144_05105"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNF65139.1"
                     /translation="MTTVAAALTALPAWHALAAHRDALAGTTLRELFARDPGRHARYS
                     LEAAGLFLDYSKNLLTDETLALLVQLAHETGVERLRTAMFAGEPINRTEGRAVLHTAL
                     RRPRGDAVFVDGANVMPAVHAVLDHMATFTEAVRSGDWRGHSGRPIEHIVNIGIGGSD
                     LGPLMACAALAPYAHPRLAMHFVSNVDGTHLAETLARVDAETTLFIVASKTFTTQETL
                     TNARSARDWLIARLGDAAAVAKHFVAVSTNAAEVAAFGIDTANMFGFWDWVGGRYSLW
                     SAIGLPIALSVGIGQFRELLDGAHALDRHFEQAPAGRNLPMLLALIGVWNANFLGAGS
                     HAVLPYDQSLHRFPAYLQQLDMESNGKRVTREGRALDYTSGPLIWGEPGTNGQHAFYQ
                     LIHQGTRLVPADFIAPIDTHHPIGRHHTLLLANCLAQSEALMRGKTEAEVRAELSAQG
                     LAGEALEALLPHKVFPGNRPSSTILMQKLTPATLGALIALYEHKVFVQGAIWNVNSFD
                     QWGVELGKQLAKAIEPELDGSREPGIHDASTAGLIARIRALRQP"
     gene            complement(2017..2745)
                     /locus_tag="PK144_05110"
     CDS             complement(2017..2745)
                     /locus_tag="PK144_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65140.1"
                     /translation="MLHTMKALLASIAKVMPLWLFWLLAGLTALSCLVALVLIFRLLM
                     RRARRGPVMIVQGPETLPDAPGGSLRCYRLDAVEALAGSGILQLLVSPSGVFAISGLA
                     VSGRLSGQARGESWRLAHRGEPRQSLPNPLLACRAQLPKLAQVLGVAPDKLHPCVLVA
                     ATARWSSSSECPVGVYFSAEELIGDVRQRLNVVFSAPERQALLARLDGLLKPSELAEP
                     VTAAPAKTARHWLRRRQPSPVQAG"
     gene            complement(2904..3575)
                     /locus_tag="PK144_05115"
     CDS             complement(2904..3575)
                     /locus_tag="PK144_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNF65141.1"
                     /translation="MSPQSPSYLTQLMPLLVSWLTVLLPMLCALLPAVLFFRGRQQGR
                     NGSLGRMLQRKLRPADGFHVYHDLEFENDYGECVRIEHLVLGSSGVYPIISTRLSGEV
                     LADIDASVPWRRMLHGREQPIPNPLARVHGAADLVSRSLGVAPACVHPLLVVTGVTRL
                     AMPPSRVAVFLSPKSACEAVHDRLALSFHDEQLQALRARLDTAQRTERPLSAPLPQAS
                     RPSVL"
     gene            3741..4607
                     /locus_tag="PK144_05120"
     CDS             3741..4607
                     /locus_tag="PK144_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF65142.1"
                     /translation="MPVQLLVPPRPTDLGGFSVRRLLPHRSCRAVGPFVFFDHMGPAD
                     FAPGHGIDVRPHPHIGLATVTWLFDGEILHRDSLGSVQPIRPGALNWMTAGRGIVHSE
                     RTGPDTRIRGGRLHGIQAWVALPEADEECEPGFVHYPAEALPQFTRNGVELILIAGTA
                     FGERSPVEVAMPTFYLEARLAAGARLMLEDEHPERAVYVAEGAARVDVRALQAGDLAV
                     LAPGGTVAIEADGPCRLMLLGGAPLDGPRTIWWNFVSSRPQRIEQAKADWRANRFAHV
                     PGESEFIPLPED"
     gene            4763..5185
                     /gene="trxC"
                     /locus_tag="PK144_05125"
     CDS             4763..5185
                     /gene="trxC"
                     /locus_tag="PK144_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNF65143.1"
                     /translation="MELNCPHCQRRNRLPPERLPELPNCGACGAPLLEGGVLELDAGS
                     FDALIAHAPRPVLVDFWAPWCSPCRQFAPTYLATAKILSERYVFAKLDTEAEPEIAAR
                     TGIRSIPTLVLFHEGHELSRRSGALPGPELRRWLLMQD"
     gene            5503..5826
                     /locus_tag="PK144_05130"
     CDS             5503..5826
                     /locus_tag="PK144_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNF65144.1"
                     /translation="MPKPTRHVLVCTQMRPPNHPRGACAHRDAGAVMNRFAEAFQARG
                     LWGSYLLSQTSCIGPCDAGTNVLVYPDNVLYSGVGTADVDEIIEAHVLGGQPVERLLA
                     APQNW"
     gene            complement(5963..6433)
                     /locus_tag="PK144_05135"
     CDS             complement(5963..6433)
                     /locus_tag="PK144_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HNF65145.1"
                     /translation="MQTLTALDARAESTVHGVRWLKLAVVYLLAGVVLGIAMGASHDF
                     TLRPVHAHLNLLGWTTLGLAGLIYAVYPQAATTRCARVHFWLFALALPVMMLALTGLL
                     LGQGAALPALVASEFAILGGVLAFALNVFLNLMPRRAASASCLPADCVPAAGRA"
     gene            complement(6699..8066)
                     /locus_tag="PK144_05140"
     CDS             complement(6699..8066)
                     /locus_tag="PK144_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNF65146.1"
                     /translation="MSGVDAIRDCLDGAIPAILATCSAEGEPNVAYLSQVEYVDDAHV
                     ALSYQFFNRTRQNVLAHPVARLIVTHPRTGARHRLLLHYLRTETGGALFERMKAKLAG
                     IASHTGMSGVFRLLGSDVYRVLSIERVPGPALAAPAPRRSPLAVLRACSESLNAYTEL
                     DELLAGALAVFERELDVRHWLLLMLDEAGERLYTLASRGYGASGIGAEIALGEGLIGV
                     AARVGTPVRIGHATAEYGYGRAIRAAAADGGLLDALETEIPLPGLADSRSQIAVPIRA
                     CGRLLGVLYAESTEDLRFGYDDEDLLVACAALLGTAMRLLQLEAHAQEGAAAAPAPPA
                     AGPAPIADAAPLTVRHYAANDSVFLDGQYLIKGVAGSILWTLLGDYVALGRTSFSNRE
                     LRVDPRIRLPGLSDNLEARLILLSRRLAEHEACVRLARTGRGRLELQVGRPLQLVEIA
                     AAEAG"
     gene            complement(8063..8596)
                     /locus_tag="PK144_05145"
     CDS             complement(8063..8596)
                     /locus_tag="PK144_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65147.1"
                     /translation="MCPDVRPHPPAVLPLAAELADFIGGAVSISLGSCGAGLVPNLTR
                     GLGCRVSADRRRLRVWLAASASAALLADVAANATLAMVCSLPATHRTVQLKTRDARIG
                     ALEAGDRDCVARYRAAFVGGLEALGYPAAVMSMLVAATDEDLAAIDFTPHAAFVQTPG
                     PAAGALLVAAGGEGHAP"
     gene            9070..10017
                     /locus_tag="PK144_05150"
     CDS             9070..10017
                     /locus_tag="PK144_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65148.1"
                     /translation="MRNDANGRTQAEALRSICLRVLPIWIRHIESVRTQSETAVSTLI
                     ARFTGLMDTLGVAIASAESGADGSREALSGLLRTGERRLLDLLDTLRAANDNRDGALA
                     PVLGLGARTDELLEMARTVTTISGQTRLLSLNATIEAVHAGEHGQGFEVVAREVRSLA
                     ARASEAGTRMETRMREIGSVLDNASAVTGECLAADRQTLADADRTLRGVLGEFGEVSG
                     RLAESTAALVGVSNNVQGEIAEVLVALQFQDRTSQILAHLCGAMQTLQRLLEQGTPDA
                     ATLEQWFTELESAYTTVEQLELHRGGPVKADSGGEITFF"
     gene            10216..10587
                     /locus_tag="PK144_05155"
     CDS             10216..10587
                     /locus_tag="PK144_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65149.1"
                     /translation="MANKNILVIDDSASLRQVVGIALKSAGYDVVEAVDGKDALGKLD
                     GRKFHLAICDVNMPNLDGIGFLKAMRELPNYRFTPVIMLTTEAGEDKKLAGQAAGARA
                     WVVKPFQPQQMLAAVAKLALP"
     gene            10599..10949
                     /locus_tag="PK144_05160"
     CDS             10599..10949
                     /locus_tag="PK144_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF65150.1"
                     /translation="MFALDRDHDADGPLLRLGGELTIYHAGELRRALLDDSAAHATVR
                     TLDLSGVEEFDSAGLQVLLMARRAAERAGGRLCLRNHSEAVREVIDRYGLAAHFGDPV
                     LIPAARADARGETR"
     gene            10946..13150
                     /locus_tag="PK144_05165"
     CDS             10946..13150
                     /locus_tag="PK144_05165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNF65151.1"
                     /translation="MNLDLELARQTFLTESRDLLVAFEDGLLALEAAGDGVPVDAEQI
                     NAVFRAAHTIKGSAGLFGYDAIVAFTHVVENALDRVRAGTLAWTRPLGGLLLDCRDHI
                     DALVACTAERGETPDAAMRSRDAELLAALQQILGGQPAAAAVPAGVAAAEVERCAGDC
                     ASADTWHISIRFGTNVLQNGMDPLTVLRYLGTLGRIVHVTTLLDALPEPAAFDAEHCY
                     LGCEIDLASSEDRETIAGAFEFVRADSVVHILPPHSRLAEYVELIRSLPEDDARLGEI
                     LVAGGALTAEELDVALGRQAAADLQAPGAHRPLGELLVDQGLVAPAVVETALDKQQRA
                     RDPRSSGGEQRQLRIRADKLDELINRVGELVIASAATALLARRNKDPELHEATAALGA
                     LVEAIRDSALNLRTVPIGETFARFRRVVHDLSRELGKDIELELSGEDTELDKSVVERI
                     ADPLTHLVRNSLDHGIEPAEVRIAHGKPARGTLRLDARHDSGSIVIEVSDDGGGLNRE
                     RILERARERGLVRAGETPGDAETLKLIFEPGFSTAPAVTSLSGRGVGMDVVRRAIEAL
                     RGSIELESEPGHGTTVRIRLPLTLAIIDGFLVGVDGATYVVPLDNVVECVELAGATAR
                     EAERNGYLNLRGRVLPLLRLRAHFGLAGEPPRRENLVVVSYAGLQVGLVVDRLLGEFQ
                     TVIKPLGRLFTQVRGFSGATILGSGAVALILDVPGLIAAGQQAARSVPGLAA"
     gene            13291..13728
                     /locus_tag="PK144_05170"
                     /pseudo
     CDS             13291..13728
                     /locus_tag="PK144_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
     gene            13927..14237
                     /locus_tag="PK144_05175"
                     /pseudo
     CDS             13927..14237
                     /locus_tag="PK144_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
BASE COUNT         2258 a         5117 c         4911 g         1951 t
ORIGIN      
        1 gtggtcgcct acagcgacgc ccacgtgcac gcgatgtcgg ccgcgctgct gaagcccggc
       61 gacgccgtgc tcgcgatctc gcagtccggc cgctcgccgg aactgctcgg cagcgcccag
      121 cgcgcccgcg ccgccggcgc cgatctggtc gcgatcaccg tcgccggctc gccgctcgcg
      181 gcgctggcga cggtcgcgat cacggtgccg gccgtggccg ctgcgccggg gccgctgccc
      241 ggcggctcgc gcgtcgccga tctcgcgatc gtcgatgcgc tcgccatcgg cgtggcgctg
      301 cgccgcaccc cgctcactcc cgaaagcccc ggactggaga cctgaccgac atgacgaccg
      361 tcgccgccgc gctgaccgcc ctgcccgcct ggcatgcgct cgccgcccac cgcgacgcgc
      421 tcgccggcac gacgctgcgc gagctgttcg cgcgcgaccc cggtcgccac gcgcgctact
      481 cgctcgaagc cgccggcctg ttcctcgact attcgaagaa cctgctgacc gacgagacgc
      541 tggcgctgct cgtgcagctc gcgcacgaaa ccggcgtcga gcggctgcgc acggcgatgt
      601 tcgccggcga gccgatcaac cgcaccgaag gccgcgcggt gctgcacacg gcgctgcgcc
      661 ggccgcgcgg cgatgcggtg ttcgtggacg gcgcgaacgt catgccggcc gtgcacgccg
      721 tgctcgatca catggcgacg ttcaccgaag ccgtgcgcag cggcgactgg cgcggccaca
      781 gcggccggcc gatcgaacac atcgtcaaca tcggcatcgg cggctccgat ctcggaccgc
      841 tgatggcctg cgcggcgctc gcgccctacg cgcatccgcg gctggcgatg catttcgtct
      901 ccaacgtcga cggcacgcat ctggccgaga cgctggcgcg cgtcgatgcg gaaacgacgc
      961 tgttcatcgt cgcctcgaag accttcacga cccaggaaac cctgaccaac gcccgaagcg
     1021 cccgcgactg gctgatcgcg cggctcggcg atgccgccgc ggtcgccaag cacttcgtcg
     1081 ccgtctcgac caacgccgcc gaggtcgccg ccttcggcat cgacaccgcc aacatgttcg
     1141 gcttctggga ctgggtcggc gggcgctact cgctgtggtc ggcgatcggc ctgccgatcg
     1201 cgctgtcggt cggcatcggg cagttccgcg agctgctcga cggcgcgcac gcgctggatc
     1261 ggcacttcga gcaggcgccg gccgggcgca acctgcccat gctgctggcg ctgatcggcg
     1321 tctggaacgc caacttcctc ggcgccggca gccacgcggt gctgccttac gaccagtcgc
     1381 tgcaccgctt cccggcctat ctgcagcagc tcgacatgga atcgaacggc aagcgcgtga
     1441 cgcgcgaggg ccgcgcgctc gactacacca gcgggccgct gatctggggc gaacccggca
     1501 ccaacggcca gcacgcgttc taccagctga tccaccaggg cacgcggctg gtgccggccg
     1561 acttcatcgc gccgatcgac acgcatcacc cgatcggccg ccaccacacg ctgctgctcg
     1621 ccaactgcct cgcgcagtcc gaggcgctga tgcgcggcaa gaccgaggcc gaggtgcgcg
     1681 ccgaactgtc ggcgcaggga ctcgcaggcg aggcgctcga agccctgctg ccgcacaagg
     1741 tgtttccggg caaccggccg agcagcacga tcctgatgca gaagctgacg ccggcaacgc
     1801 tcggcgcgct gatcgcgctg tacgagcaca aggtgttcgt gcagggcgcg atctggaacg
     1861 tcaacagctt cgatcagtgg ggggtcgagc tcggcaagca gctcgcgaag gcgatcgagc
     1921 cggaactcga cggcagccgg gaacccggca tccacgatgc ctcgaccgcc gggctgatcg
     1981 cgcgcatccg cgccctgcgc cagccctgag gctgcttcag ccggcctgca ccggcgaggg
     2041 ctgccggcgc cgcagccagt ggcgagcggt cttcgccggc gcggccgtga ccggctcggc
     2101 gagttccgag ggcttcagca gcccgtcgag ccgggcgagc agcgcctggc gctccggagc
     2161 cgagaacacc acattcaggc gctggcgcac gtcgccgatc agttcctcag ccgagaaata
     2221 cacgccgacc gggcactcgg atgacgacga ccagcgcgcc gtcgccgcca ccagcacgca
     2281 gggatgcagc ttgtcgggcg cgacgccgag cacctgggcg agcttcggca gctgcgcgcg
     2341 gcacgcgagc agcggattcg gcaaggactg gcgcggctcg ccgcgatgcg cgaggcgcca
     2401 ggactccccg cgtgcctggc cgctgagccg gccgctgacc gccagcccgc tgatcgcgaa
     2461 cacgccggac ggcgacacca gcagctgcag gatgccgctg ccggccagcg cctcgacggc
     2521 atcgagccgg tagcagcgca gcgatccgcc cggggcatcc ggcagggttt cgggcccctg
     2581 gacgatcatg accggcccgc ggcgcgcacg gcgcatcagc agccggaaga tcagcaccag
     2641 cgccacgaga cagctcagcg cagtcagccc cgcgagcaac cagaacagcc agagcggcat
     2701 caccttggcg atggatgcca gcaacgcctt catcgtatgc agcatgtaca cagtcctttg
     2761 ccggggcggt cggggaaatc acaacggccg gagccgggtc agaccggcgg ggcgaagcag
     2821 aatttcagca taccggcaaa cgcaaggcgc cggacgcccg caagcggacg tccggcgccg
     2881 gagcagccgt gcggatcagc ggatcagagc acggacgggc ggctcgcctg cggcagcggc
     2941 gccgacagcg gccgctcggt gcgctgggcg gtgtcgagcc gggcgcgcag cgcctgcagc
     3001 tgttcgtcgt gaaagctcag ggccagccgg tcgtgcacgg cttcgcaggc ggacttcggc
     3061 gacaggaaca cggcaacgcg gctcggcggc atcgccaggc gcgtgacgcc ggtgacgacc
     3121 agcagcggat gcacgcaggc cggcgcgacg ccgaggctgc ggctgaccag atcggcggca
     3181 ccgtgcacgc gcgcgagcgg attcgggatc ggctgctcgc gcccgtgcag catgcgccgc
     3241 cacggcacgg aggcatcgat gtcggcgaga acctcgccgc tcagccgggt gctgatgatc
     3301 ggatagaccc cgctcgaccc gagcacgagg tgctcgatgc gcacgcactc gccgtaatcg
     3361 ttctcgaatt cgagatcgtg atagacgtgg aagccatcgg ccggccggag cttgcgctgc
     3421 agcatgcgtc cgagcgaacc gttgcgcccc tgctggcggc cccggaagaa gagcacggcc
     3481 ggcagcaggg cgcagagcat gggcagcagc acggtcagcc aggaaaccag cagcggcatc
     3541 agctgcgtca ggtacgacgg gctctgcggg gacatcgagg gcaaatcctc agctgcagcg
     3601 gggttgttcc ggaaacgcgc gggattgtca tatgccgcgc cttgccgccg ggcctgtcga
     3661 ctgaaattgt cgcaatcttt aacgcaggag ccgcgcatgt ccgatgcttg agcacccttc
     3721 tccctgcgga gttccccgcc atgcccgtgc agctgctcgt tccgccccga ccaaccgatc
     3781 tcggcggctt cagcgtccgc cggctgctgc cgcaccgcag ctgccgggcg gtcggcccct
     3841 tcgtgttctt cgatcacatg ggaccggccg acttcgcccc cggtcacggc atcgacgtgc
     3901 gtccgcatcc gcacatcggg ctcgcgaccg tcacctggct gttcgacggc gagatcctgc
     3961 accgtgacag cctcggctcc gtgcagccga tccgccccgg ggcgctgaac tggatgaccg
     4021 ccgggcgcgg catcgtgcat tccgagcgca ccggtccgga cacccgcatc cgtggcggcc
     4081 gcctgcacgg catccaggcc tgggtggcgc tgccggaggc cgacgaggaa tgcgaaccgg
     4141 gcttcgtgca ttacccggcc gaggcgctgc cgcagttcac gcgcaacggc gtcgagctga
     4201 tcctgatcgc cggcacggcc ttcggcgagc gctcgccggt cgaggtcgcg atgccgacct
     4261 tctacctcga agcccgcctc gctgccggtg cccggctgat gctcgaggac gagcacccgg
     4321 agcgcgccgt ctacgtcgcc gagggcgcgg cgcgggtgga tgtccgcgca ctgcaggccg
     4381 gcgatctggc ggtgctcgcc cccggcggca cggtcgcgat cgaggccgac gggccgtgcc
     4441 ggctgatgct gctcggcggg gcaccgctcg acggaccgcg cacgatctgg tggaacttcg
     4501 tttccagccg cccgcagcgc atcgagcagg ccaaggccga ctggcgcgcg aaccgcttcg
     4561 cccacgtgcc cggcgagtcc gagttcatcc cgctgcccga ggattgagcc gcccgcaggc
     4621 cgggcagacg ccccccgctt cgcccccgcc cggcgttgtc gcccgccccg cggggcgtca
     4681 ggcgatcggc aggccattga aatcaggccg gtcgccagca gattcatggt ctccgcccac
     4741 gatcggccca ggattcccgg acatggaact gaactgcccg cactgccaga gacgcaatcg
     4801 cctgcccccc gaacgcctgc ccgaactgcc gaactgcggt gcctgcgggg caccgctgct
     4861 cgaaggcggc gtgctcgaac tcgatgccgg cagcttcgac gccctgatcg cccacgcccc
     4921 gcgcccggta ctggtggatt tctgggcacc gtggtgcagc ccgtgccgcc agttcgcgcc
     4981 gacctacctg gcaacggcga agatactgtc cgagcgttac gtgttcgcga agctcgacac
     5041 cgaagccgaa ccggaaatcg ccgccaggac cggcatccgc agcattccga cgctggtgct
     5101 gtttcacgaa ggccacgaac tgtcgcgccg cagcggcgcg ctgccgggcc cggagctgcg
     5161 ccgctggctg ctgatgcagg actgaagtcc cgatccgccg ctccggcatc ttcgggccga
     5221 ccgggggaat cgaggccgct gaccggggtc ggcgggcgga ccgcagtggg ggtccggcca
     5281 gtcccggccc ggtgcttcgc gggtggcggc ggacgcccga tccggacacc gggtctggcc
     5341 ggctgtccgg accccgacag caccgaccgt ttccggacgc cggtgcgaca ttgacccgct
     5401 tgcaccaggc cactgacaaa taacgagtat caacgaatat cagggaatgg caccgtttgt
     5461 gctgcgctgc aatacatacc gacaacagga gatcgcagcg ccatgccgaa accgacccgc
     5521 cacgtcctcg tctgcacgca gatgcgcccg ccgaatcacc cgcgcggtgc ctgcgcgcat
     5581 cgtgatgccg gtgccgtgat gaaccgcttc gccgaagcct tccaggcccg cgggctgtgg
     5641 ggcagctacc tgctgtcgca gaccagctgc atcggtccct gcgatgccgg cacgaacgtg
     5701 ctcgtctacc cggacaacgt cctgtacagc ggcgtcggca ccgccgatgt cgacgagatc
     5761 atcgaagccc acgtgctcgg tggccagccg gtcgagcgcc tgctcgccgc gccgcagaac
     5821 tggtaagccc cgcctgcaga cgaccggccg ctccccgtgc cctgcgctgc tgcgacgggc
     5881 tccgggtgag cggccggatt ttttcgggat tttcccgggc tccttcaggg gtgagcggcc
     5941 gggcgggaaa gcggccggac gttcaggcgc gaccggcggc cggcacgcag tcggccggca
     6001 ggcagctggc actggccgca cgacgcggca tcaggttcag gaacacgttc agcgcaaagg
     6061 cgagcacgcc gccgaggatc gcgaactccg aggccaccag cgccggcagg gccgcgccct
     6121 ggccgagcag caggccggtc agcgccagca tcatgaccgg cagcgccagc gcgaacagcc
     6181 agaagtgcac gcgcgcacag cgcgtcgtcg ccgcctgcgg atagaccgcg tagatcagcc
     6241 cggccagccc cagcgtcgtc cagccgagca ggttcaggtg cgcatgcacc ggacgcagcg
     6301 tgaagtcgtg gctggcgccc atggcgatgc cgagcacgac gccggcgagc aggtagacga
     6361 cggcgagctt cagccagcgc acgccgtgga cggtggattc ggcgcgggca tcgagggcgg
     6421 tgagggtctg catgatggaa ctccggatcg gacaggttcg ggaaaggcct ggaaaggtct
     6481 ggatcaggtt tcgggaggca tccgtgcccg cgcaccggct cgcggaccgg gcggctgcgg
     6541 atacggcctg gtcggcaagt cccggtacgg agccggtttt ctgcttcggg ccgcagtgcg
     6601 ggacggattc tggctgcagg ggccgggcga tacatccggc ccgcgccgta gtgcagcgaa
     6661 tcttcggatt cctttaggaa acttttagga tcggccgctc agccggcctc ggcggcggca
     6721 atctcgacca gctgcagcgg gcgtccgacc tgcagttcga gccggccgcg gccggtgcgc
     6781 gccagccgca cgcaggcctc gtgctcggcg agccggcgcg acagcaggat cagccgggct
     6841 tcgaggttgt cgctcaggcc cggcagccgg atgcgcgggt cgacgcgcag ttcacggttg
     6901 ctgaagctcg tgcgcccgag cgcgacgtag tcgccgagca gcgtccagag gatcgaaccg
     6961 gccacgccct tgatcaggta ctggccgtcg aggaacacgc tgtcgttggc cgcgtaatga
     7021 cgcacggtca gcggcgctgc atccgcaatg ggcgccgggc cggcagcggg cggggccggg
     7081 gcggcggcgg cgccctcctg ggcatgggct tcgagctgca gcaggcgcat cgcggtgccg
     7141 agcagggccg cgcaggcgac cagcaggtcc tcgtcgtcgt agccgaagcg caggtcctcg
     7201 gtgctctcgg cgtacagcac gccgagcagc cggccgcagg cgcggatcgg cacggcgatc
     7261 tggctgcgcg aatcggcgag gcccggcagc gggatttcgg tttcgagcgc atcgagcagg
     7321 ccgccgtcgg cggccgccgc gcggatcgcg cggccgtagc cgtattcggc cgtggcatgg
     7381 ccgatgcgga ccggcgtgcc gacgcgcgcg gcaacgccga tcaggccctc gccgagcgcg
     7441 atctcggcgc cgatgcccga ggcgccgtag ccgcggctcg cgagcgtgta caggcgctcg
     7501 ccggcttcat cgagcatcag cagcagccag tggcgcacgt cgagttcgcg ctcgaacacg
     7561 gccagcgcgc cggcgagcag ttcgtcgagt tcggtgtacg cattcaggct ttccgagcag
     7621 gcgcgcagca ccgccagcgg cgaacgccgc ggcgcgggtg cggcgagtgc cgggccgggc
     7681 acgcgctcga tcgacagcac gcgatagaca tcggagccga gcagccggaa cacgccgctc
     7741 atgccggtgt gcgaggcgat gccggcgagc ttcgccttca tgcgctcgaa cagcgccccg
     7801 ccggtctcgg tgcgcaggta gtgcagcagc aggcggtgac gcgcgccggt gcgcggatgc
     7861 gtgacgatca gccgcgcgac cggatgcgcg agcacgttct gccgcgtgcg gttgaagaac
     7921 tggtacgaca gcgcgacgtg cgcatcatcg acgtactcga cctgcgacag atacgcgacg
     7981 ttcggctcgc cctcggccga gcaggtggcg aggatggccg ggatcgcgcc gtccagacag
     8041 tcgcggatgg catcgacgcc gctcacggcg cgtgtccctc gccgccggcg gccacgagca
     8101 gcgcaccggc tgccggcccc ggcgtctgca cgaaggccgc atgcggcgtg aagtcgatcg
     8161 cggcgagatc ctcgtcggtc gcggcaacca gcatggacat gaccgcggcc gggtaaccga
     8221 gcgcttcgag gccgccgacg aaggccgccc ggtagcgcgc aacgcagtcg cgatccccgg
     8281 cttcgagggc gccgatgcgg gcgtcgcggg tcttgagctg caccgtgcgg tgcgtcgccg
     8341 gcaggctgca gaccatcgcc agcgtcgcgt tcgccgccac gtcggcgagc agcgccgccg
     8401 aggcgctggc cgcgagccag acgcgcagcc ggcggcggtc cgccgatacc cggcagccga
     8461 ggccgcgcgt caggttcggc accagcccgg caccgcagct gccgaggctg atcgataccg
     8521 ctccgccgat gaaatccgcc agttcggcgg cgagcggcag cacggcaggc gggtgcgggc
     8581 ggacatcggg acacatggcg gctaccgctt ggtcggcttt tctggcgaca gcgtaggtgc
     8641 tggcctgcgc gcctgcgccg ggtcgcggcg caggcggctg tcagcaaatc ttacacatga
     8701 caaaaattgt tccgcgacgc ttgccgtgct tgtgcgccga tatagtgccg gacaaacaga
     8761 ttgcgacctg cccgcagaaa catccggggg tcgtgcaatg cagcaggaca tgcagatgaa
     8821 ttcgtgattt ccggaaaaac ccgggctgcc attcagaaaa ccgggacgga gccgatgaag
     8881 ccgtcacggc atcgactgca tgccggcaat ccctgatctc aggcgtttca gatatgttcc
     8941 ggacgaataa gcatgagcac gggtggagcc attcggacat tgcagcactg gttcaaaaag
     9001 cggccggccc cggaggccgg cgaattccgg gaccggcacg atgcgtcagc catgacgatc
     9061 ggacatgaca tgcgaaatga tgcgaacggc cggacgcagg ccgaggcact gcgcagcatc
     9121 tgcctgcgcg tgctgcccat ctggatccgg cacatcgaga gcgtgcgcac gcaatcggaa
     9181 acggcggtat cgacgctgat cgcgcgcttc accgggctga tggacacgct cggcgtggcg
     9241 atcgcctcgg ccgagtccgg cgccgacggc agccgcgagg cgctctccgg gctgctgcgc
     9301 accggcgagc gccgtctgct cgacctgctc gacacgctgc gcgccgccaa cgacaaccgc
     9361 gatggcgcgc tcgcaccggt gctcgggctc ggcgcgcgca ccgacgaact gctggagatg
     9421 gcgcgcacgg tcacgaccat ctccggccag acgcggctgc tgtcgctgaa cgcgacgatc
     9481 gaggccgtgc acgccggcga gcacggccag ggcttcgagg tcgtcgcccg cgaggtgcgc
     9541 agcctcgccg cgcgcgccag cgaggccggc acgcgcatgg aaacgcgcat gcgcgagatc
     9601 ggcagcgtgc tcgacaacgc ctcggccgtg accggcgaat gcctggccgc cgaccgccag
     9661 accctcgccg atgccgaccg cacgctgcgc ggcgtgctcg gcgagttcgg cgaggtttcg
     9721 gggcggctcg ccgaatcgac cgcggcgctg gtcggcgtca gcaacaacgt gcagggcgag
     9781 atcgccgaag tgctggtggc gctgcagttc caggaccgca ccagccagat cctcgcgcac
     9841 ctgtgcgggg cgatgcagac gctgcagcgc ctgctcgaac agggcacccc ggacgccgcg
     9901 acgctggagc agtggttcac cgaactcgag agcgcctaca cgaccgtcga acagctcgaa
     9961 ctgcaccgcg gcggtccggt gaaggccgat tccggcggcg agatcacctt cttctgacga
    10021 acttctgacg aacgcgcgct ttccggccgg tgcgcagcag gaaatcagga aaccggaagt
    10081 caccagattc gggtcgtcgc cccggcgacg ccccctcaag aaaccggatc acgatccgat
    10141 atcacctgca gacggatgcg tcattgcagc gcatccggcc gcttcgccat ctgacggcaa
    10201 caggagtgaa ttcccgtggc taacaagaac atcctcgtca tcgacgattc cgccagcctg
    10261 cgccaggtgg tcggcatcgc tttgaaaagc gccggttatg acgtggtcga ggccgtcgac
    10321 ggcaaggacg cactcggcaa gctcgacgga cgcaaattcc atctcgcgat ctgcgacgtg
    10381 aacatgccga atctcgacgg catcggcttt ctgaaagcca tgcgcgagct gccgaattac
    10441 agattcacgc cggtgatcat gctcaccacc gaagccggcg aggacaagaa gctcgccggc
    10501 caggcggccg gggcacgggc ctgggtggtc aagcccttcc agccgcagca gatgctggcg
    10561 gcggtcgcca agctggcgct gccctgagtc ccgccgccat gttcgcgctc gatcgggatc
    10621 acgacgccga cggcccgctg ctgcgcctcg gcggcgagct gaccatctac cacgccggcg
    10681 aactgcgccg ggcgctgctc gacgactccg ccgcccacgc caccgtgcgc acgctcgatc
    10741 tgtcgggcgt cgaggaattc gattcggccg gcctgcaggt gctgctgatg gcccggcgcg
    10801 cggccgagcg cgccggcggc cggctgtgcc tgcgcaacca cagcgaggcc gtgcgcgagg
    10861 tcatcgaccg ctacggcctc gccgcgcatt tcggtgatcc ggtgctgatt ccggccgccc
    10921 gtgccgatgc ccgcggagaa acgcgatgaa cctcgatctc gaactcgcgc gccagacctt
    10981 cctgaccgag agccgcgacc tgctggtggc cttcgaggac ggcctgctcg cgctcgaggc
    11041 cgccggcgac ggcgtgccgg tcgatgccga acagatcaac gcggtgttcc gcgccgcaca
    11101 cacgatcaag ggctcggcgg ggctgttcgg ctacgacgcc atcgtcgcct tcacgcacgt
    11161 cgtcgagaac gcgctcgatc gcgtgcgcgc cggcacgctg gcctggacgc gcccgctcgg
    11221 cgggctgctg ctcgactgtc gcgaccacat cgatgcgctc gtcgcctgca ccgccgagcg
    11281 cggcgagacg ccggacgccg cgatgcgcag ccgcgatgcc gagctgctcg cagcgctgca
    11341 gcagatcctc ggcgggcaac cggccgctgc tgccgtgccg gccggcgtcg ccgcggccga
    11401 agtcgaacgc tgcgccggtg attgcgcgag cgccgacacc tggcacatct cgatccgctt
    11461 cggcacgaac gtgctgcaga acggcatgga cccgctgacg gtgctgcgct acctcggcac
    11521 gctcgggcgc atcgtgcacg tgacgacgct gctcgatgcg ctgcccgagc cggcggcctt
    11581 cgatgccgag cactgctatc tcggctgcga gatcgacctc gcatccagcg aggaccgcga
    11641 gaccatcgcc ggggccttcg agttcgtgcg cgccgacagc gtcgtgcaca tcctgccgcc
    11701 gcacagccgg ctggccgaat acgtcgagct gatccgctcg ctgcccgagg acgacgcccg
    11761 gctcggcgag attctggtcg ccggcggcgc gctgaccgcc gaggagctcg atgtcgcact
    11821 cggccgtcag gccgctgctg acctgcaggc gcccggagcg caccgcccgc tcggcgaact
    11881 gctggtcgat cagggcctgg tcgcgccggc cgtggtcgag accgcgctcg acaagcagca
    11941 gcgcgcccgc gatccgcgca gcagcggcgg cgagcagcgc cagctgcgca tccgcgccga
    12001 caagctcgac gaactgatca accgcgtcgg cgaactcgtg atcgccagcg ccgcgaccgc
    12061 gctgctcgcg cggcgcaaca aggacccgga gctgcacgag gcgaccgccg cgctcggcgc
    12121 gctggtcgag gcgatccgcg acagcgcgct caacctgcgc acggtgccga tcggcgagac
    12181 cttcgcgcgc ttccggcgcg tcgtgcacga tctcagccgc gaactcggca aggacatcga
    12241 gctggagctt tccggcgagg acaccgaact cgacaagtcg gtggtcgagc gcatcgccga
    12301 tccgctgacc caccttgtgc gcaattcgct cgatcacggc atcgaaccgg ccgaggtgcg
    12361 catcgcccac ggcaagccgg cacgcggcac gctgcgcctc gacgcccgcc acgactccgg
    12421 cagcatcgtc atcgaggtca gcgacgacgg cggcggcctg aaccgcgagc gcatcctgga
    12481 gcgcgcccgc gagcgcggcc tcgtgcgcgc cggcgaaacc ccgggcgatg ccgagacgct
    12541 gaagctgatc ttcgagccgg gcttctcgac cgcgccggcg gtcaccagtc tttcgggccg
    12601 cggcgtcggc atggacgtgg tcaggcgcgc gatcgaggcg ctgcgcggca gcatcgagct
    12661 cgaatccgaa cccggccacg gcacgacggt gcgcatccgc ctgccgctga cgctcgcgat
    12721 catcgacggc ttcctggtcg gcgtcgacgg cgcgacctac gtcgtgccgc tcgacaacgt
    12781 cgtcgaatgc gtcgagctcg ccggtgcgac ggcgcgcgag gccgagcgca acggctacct
    12841 gaacctgcgc ggcagggtgc tgccgctgct gcgcctgcgc gcgcacttcg ggctcgccgg
    12901 cgagccgccg cggcgcgaga acctggtcgt cgtcagctac gccggcctgc aggtcgggct
    12961 ggtcgtcgac cgcctgctcg gtgaattcca gaccgtcatc aagccgctcg ggcgcctgtt
    13021 cacgcaggtg cgcggcttca gcggcgccac gatcctcggc tccggcgccg tcgcgctgat
    13081 cctcgatgtc cccggcctga tcgccgccgg ccagcaggcg gcacgttccg tccccggcct
    13141 cgctgcctga cgcctctcac gccttccccc tttcccccgc cagagcgcaa gaaccggccg
    13201 cgcagacgac cggcaccaaa ggagtcacca cagatgcaat tccttgcccg gatgaagatt
    13261 tccacgcgca tgacgctggt catcgccttc atgctgacgc tgctcgccgg cctcggcggc
    13321 tttgcgatcc agcagctcac gcacttcaac cgcatcgtca ccgacgtggc cgagaactgg
    13381 ctgccgagcg tgcagacgat ctcggcgatg gacaccgcgg tctcggtgtt ccgcatccgc
    13441 gagttcaagc acgtgctgac cgaagacctg ccgggcatgg acaaggtcga ggcgatgatg
    13501 ggcgagtccc tcgacagctt cgagaagaac cgcaaggtct atgcggcgct gatctcctcg
    13561 cccgaggaac agatgctgta cgacgaattc tcgaagcagt ggaacaccta cctggccctg
    13621 cacgccgaac tgcttcagct gtcgcgtggc aaccgcaccg ccgaagcgcg tgaactgatc
    13681 aacggccgca tgttcgacgc cttccagaag gccacggccg acctgcagaa actcgtcgac
    13741 ctgaacgtca acggcggcaa tgtctcgcgc aaggaagccg ccgaaacctt ccagcactcg
    13801 cgcgacacga tcctgttcgc gctcggcggt gccttcgcgc tggcggtgct ggtgtccttc
    13861 ctgaccgtgc gcagcatctc gcgccggctc ggcgatgcgc tggccgtcac ccgccagctc
    13921 gccggccagg tcagctcgac ctcgcatgcg ctgtcgcagg ccaccagcga gcaggcggcc
    13981 tcggtcgagg agacctcggc ggcgatcgag cagatggcgg cctcgatcac gcagaacagc
    14041 gagaacgccc gcgtcaccga cggcatggcg cagcgcgccg ccgaacaggc cgcggccggc
    14101 ggcgaggccg tgcgccagac cgtcacggcg atgaagagca tcaccgagcg catctcgatc
    14161 atcgacgaca tcgcctacca gaccaacctg ctcgcgctca acgccgcgat cgaggccgcg
    14221 cgcgccggcg agcacgg
//
LOCUS       DAOQIE010000053        14223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2043_length_14223_cov_7.867307, whole genome shotgun sequence.
ACCESSION   DAOQIE010000053 DAOQIE010000000
VERSION     DAOQIE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_14223_cov_7.867307"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4977
                     /locus_tag="PK144_05180"
     CDS             <1..4977
                     /locus_tag="PK144_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNF65152.1"
                     /translation="PADGEAELLRWRALLTRQLDRIDAELAALAGADGSRTLAALAAG
                     DGPVAVLARTRLQQLEALAERCGRFAGAMDFRFLYDPARRLLTIGFNQSTMHRDTSYY
                     DLLASEARLTSFVAIALGQLPQESWFSLGRRLTAIGGEAALVSWSGSMFEYLMPPLVM
                     PEYRGTLLERTCAAAVARQIAYGRQRNVPWGISESGYNATDAQFNYQYRAFGVPGLGL
                     KRGLGDDLVIAPYATLMALPVDPPAAVRNLQRMAEAGFLGRHGFYEAIDYTPHRLPPN
                     VTHAVVRSFMAHHQGMAFLALAQTRLGPLMQQRFAADKALCATLLLLQERVPASAAYR
                     SSEEAVGSGPKPAAAGTLPERPNRSFDTADTALPQLVVLSNGSLNVLLTQAGGSRLER
                     RGLALTRWREDGTCDDRGLFFYLRDTASGAVWSAGFQPVRVRPPVFSATFSEGRAEFL
                     RRDGDIELRMEVVVSPEDDIELRRLHLTNRGTSVRSIELTSFAEVALAPPAADAAHPA
                     FSKLFVQTEAVPEAHALLATRRARGHGEAQPWLLHALLPRPEDPNTALAPASFETDRA
                     RFIGRTRSSGAPLALAGAPGPLSGTTGAVLDPVIAIRHVFTIRPGESMIVDALTGGAD
                     SREAAQTLLQKYRDPHMADRVFDLAWIHSQVVLRQLGVTETDAQLYWELAACVVFSDA
                     HGRADAATLRQNRRGQSGLWSHAISGDLPIVLLRIQDVSNLDLARQLIQAHAYWRSKG
                     LVADLVILNEDHASYRPLLLEQITGLIAAGIEASSTDKPGGIYVRNAEHVSHEDRVLL
                     ESVARFIAWDRKGTLEQQLRRSATQPLLPPVLAPAADAVAPAAPVSLVREDLLLDNGF
                     GGYTADGREYLIRLRPDAPTPLPWANVIANAEGFGTVISECGSAYTWAVNAHEYRLTP
                     WHNDPVSDSCGEAFYVRDEDSGRYWSPTPLPARGRGDYLCRHGFGYSVFEHIEDGIVS
                     ELAVWVGAGLPVKFSRVMLRNDSGRPRRLSLTGYVEWVLGEHRWKSAPHVITECDAIC
                     GALLAHNAYSSEFPHMSAFFDADPGPQPGARSYTTDRTEFIGRNGSLAAPAAMGRTTL
                     GNRTGAGLDPCAAIRLPLTLAPGEQVELVFRLGAAPTHEVAQRAVAMRTPGVAWREWR
                     AMQLHWEALLDTVQIDTPDPAVNLLGNGWLVYQVLGCRMQARSGYYQSGGAFGLRDQL
                     QDAMALVHAAPQRLRAQLLLAASRQFIEGDGQHWWHPPGGRGVRTRCSDDYLWLPLAT
                     CRYVGATGDTGVLDEVLPFLEGRPVPDGEESYYDLPSDSQQAASLYEHCRRAIRTGLR
                     YGGHGLPLMLAGDWNDGMNLVGIDGRGESVWLGFFLYTVLQDFAPLAARRGDDEFATL
                     CRREAATLRDNLERHAWDGEWYLRAWFDDGSPLGSQASAECRIDAIAQSWAVLSGAAD
                     PARSAQAMDSLERRLVRREAGIIQLFDPPFSGHGPLDPGYIRGYLPGVRENGGQYTHS
                     AVWAVMAFAALGERQRAWELFDLINPLNRTRDAAGIERYRAEPYVMAADVYGVEPHTG
                     RGGWTWYTGSAGWMYRLLLESLLGLTRHADALRLDPLLPDGWPGYTLTYRHGASRWHI
                     EVSRGRGGEGAQLSVDGRVRSDGRIPLVDDGAEHRVELKLDGRQRQAA"
     gene            5448..5804
                     /locus_tag="PK144_05185"
     CDS             5448..5804
                     /locus_tag="PK144_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65153.1"
                     /translation="MFRGILGAALLAAVTFVLPARAGDQDFTLVNRTGVEIHKVYASP
                     HSSDDWEEDILGDDTLPDGESVNITFSPKERAAMWDLRVEDSDGNFITWESLDLLQIS
                     RVTLHYKKGKVWATLD"
     gene            complement(5823..7067)
                     /locus_tag="PK144_05190"
     CDS             complement(5823..7067)
                     /locus_tag="PK144_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF65154.1"
                     /translation="MRNPFRALASANYRRYWLGQSVSLLGTWLQQVAMGWLAWRLSGS
                     PALLGLIGFCANAGVLVLGPFAGVLVDRIDRRRALWTTQCLAAVQAGGLAALTLAGRI
                     EVWHLVALALALGAISALDLPLRQALQAELVGDRTLLPNAIALNSLSFNVARALGPAL
                     AGALVAALGEGVCFALNALSYVAVLAALARIDWPPPPPRAPPVPFAEHWREGLRYARD
                     FAPLAAALGLLAPLSLAVGPYATLMPVVAREVYGGGPHTLGWLLSAAGAGAIAGTLAL
                     AARERSSGYERIAAASALAAATGLLGLAAEPPYALALGMMMLIGGGMMTTAAALNSLL
                     QTVVEPRLRGRAMSLYVLAFAGVFPLGALMSGLLAGHFGVQATLALAGLGALAVVLHY
                     LRRLPRLSARLAGEYRRLDLDG"
     gene            7220..7963
                     /locus_tag="PK144_05195"
     CDS             7220..7963
                     /locus_tag="PK144_05195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNF65155.1"
                     /translation="MALPPDARRPGADVLADPAGYFEDLERVAAGSTALPALRCLLVT
                     AALRDDDTGRHTVRVGYMAELIGRRLGLDEDTCLRLRASAPLHDIGKLAIPDAILNKP
                     GPLDATEMAVMRRHAEFGERILASTGEPLFRMAADIAGAHHEHYDGSGYPRGLRRDAI
                     PLAARITAVCDFYDALRMDRVYRKALPDATVLGWMQEQRGRAFDPQVLDAFSDCCGEL
                     AEAHDWLEEQAARLFTMLRAQAAGQSPPV"
     gene            complement(7990..8271)
                     /locus_tag="PK144_05200"
                     /pseudo
     CDS             complement(7990..8271)
                     /locus_tag="PK144_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(8673..10415)
                     /gene="msbA"
                     /locus_tag="PK144_05205"
     CDS             complement(8673..10415)
                     /gene="msbA"
                     /locus_tag="PK144_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNF65156.1"
                     /translation="MTSRELYLRLLTFVRPHARMFALSLAGTVVLALCEPGIAALLKP
                     LLDGSFVQKDPELMRTVPLMLVGLFLVRSAADFVSDVAMKWVATRVVMDLRQALFGKL
                     LQMPTAFYDGHATATLLSRVSYETNQVMNACTDALVTMVKDGLTVIGLLCWMMYLNWK
                     LSLLTLAIVPVIALVMRLVSKRLRLLSRRLQREVGEVNRVLEEALSGHKVVKIYGGQD
                     YESARFFKTSNWVRRLQMKLATSSNAAIPGVQLFGVLALAAVIYLASLESLEGDITVG
                     GFVSLFGAMAMMFAPIKRLTKVNDQLQRGLAAAESIFALLDAEPEADTGTRTIGRAQG
                     AVRFEGVRFAYDRNGAGVVDGIDLAVDPGETIALVGASGSGKTTLMALLPRLYELSAG
                     RITLDGTDIRELTLASLRANIAYVSQETVLFNDTVAANIAYGHGTPPSRADIEAAAEA
                     AHALEFIRELPAGLDTVIGERGMRLSGGQRQRLAIARALLKNAPILILDEATSALDTQ
                     SERLVQQALERLRAGRTAFVIAHRLSTIESADRIVVLAKGRIVETGTHATLLAAGGVY
                     AGLHRLQQTEPPMS"
     gene            10612..11799
                     /locus_tag="PK144_05210"
     CDS             10612..11799
                     /locus_tag="PK144_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF65157.1"
                     /translation="MSSSTVHVDVLFVGGGIAGLWLLNCVRQAGYSALLVESERIGAG
                     QSGASQGFVVHRPRTGATVPTDKEAAELASVVGRWRELLAGRAVPDLSGVSVLSEHPL
                     RWTLPGQPGRSLLDRLLRRSPPLPDAAAETGFDIPGVLRHPMRRGSLSTQEGFIVDNR
                     SLLGALLDPVRDALVHTQGAAVMARDGGFNLRIEEREPVVVRARRTVYTTGVGANLLG
                     SVSLQVWHQQMVMIRGEGLADDLYINAVNEAGRRLFNISSHRDLTGRPVWYYGGAMVE
                     SGEQVDAARQIRNCRRDLGAYFPWMALSGLEFAAHRVDRAAVRMRNGLALLPQLRVED
                     QRVVAWPTNLSTMPLLGDRMLAMLRREGIEPRPADLHLLADWPQPALAPAPWDEEAQT
                     WQS"
     gene            11787..13109
                     /gene="waaA"
                     /locus_tag="PK144_05215"
     CDS             11787..13109
                     /gene="waaA"
                     /locus_tag="PK144_05215"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNF65158.1"
                     /translation="MAELNARAATPAPSRRERLARRLWSGLVAALVPLALARLWWRGR
                     RSPGYRERFAERFGAAPRLAPGAIWLHAVSLGETRAALPLIRALLARHPDRPLLVTTT
                     TPTGSAQVREALGERVAHCYAPWDLPGACARFLDRAQPALAVIMETELWPNLFAACAA
                     RGVPLVVANARLSARSARGYARVPWLIRPTLAATTLIAAQAEADAGRFVALGAPRVGV
                     SGNLKFDLALPEALPARAQALRRHWGAARPVWIGASTHAGEDELLLDAHARLRERWPE
                     LLLLLVPRHPERFDAVAALVQARGFALARRSHGETGQAAAVYLGDTMGELLLLAAASD
                     AAFVGGSLVPTGGHNVLEPAALGVPVAWGPHMFNFAAAEQLLAGAGAGRQVSDAGALV
                     QTLADWLADPALRARCGAAGAAVVAANRGALARLLERLDALLPPAAAP"
     gene            13249..>14223
                     /locus_tag="PK144_05220"
     CDS             13249..>14223
                     /locus_tag="PK144_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF65159.1"
                     /translation="MSGFPRFPRRWLAGLALAVTSALVHAADTWPLPPAGSQLIGETR
                     IEYAKQEDTFIDIAQRNGLGYDEIVAANPGVDRWLPGEGTPVILPLQHILPDAPREGI
                     VVNLPEMRLYYFPRGQNIVKSYAISIGRMDWHSPLGRTLVTAKVVNPTWTPPASIKAE
                     HLAKGDPLPDVVPGGPDNPLGTRAMRLGIPGYLIHGTNNPNGIGMRVTHGCIRMYPDD
                     IERLFPDVPVKTPVTLVNQTAKLGWQNGVLYLEYHSPLEEDEIPTQQQIDETVQLIHS
                     KIGDRVPLVSPDIVQTVVRQASGMPVAISLPVAIDPSQLPPPASEPVPP"
BASE COUNT         1918 a         5184 c         5193 g         1928 t
ORIGIN      
        1 ccggccgacg gcgaggccga gctgctgcgc tggcgcgcgc tgctgacgcg ccagctcgat
       61 cgcatcgacg ccgaactcgc ggcgctcgcc ggcgccgacg gcagccggac gctcgccgca
      121 ctcgccgccg gcgacggccc ggtcgcggtg ctggcgcgga cgcggctgca gcagctcgaa
      181 gccctcgccg agcgctgcgg gcgcttcgcc ggcgcgatgg acttccgctt cctgtacgac
      241 ccggcgcggc ggctgctgac gatcggcttc aaccagtcga cgatgcatcg cgacaccagc
      301 tactacgacc tgctcgcctc cgaggcgcgc ctgaccagct tcgtcgccat cgccctcggc
      361 cagctgccgc aggagagctg gttctcgctc gggcggcggc tgaccgcgat cggcggcgag
      421 gcggcgctgg tgtcgtggag cggctcgatg ttcgagtacc tgatgccgcc gctggtgatg
      481 ccggagtacc gcggcacgct gctggagcgc acctgcgcgg ccgccgtggc gcgccagatc
      541 gcgtacggcc gccagcgcaa cgtgccctgg ggcatctcgg aatccggcta caacgccacc
      601 gatgcgcagt tcaactacca gtaccgcgcc ttcggcgtgc cgggcctggg cctcaagcgc
      661 ggcctgggcg acgatctggt catcgcgccg tacgcgacgc tgatggcgct gccggtcgat
      721 ccgccggcgg ccgtgcgcaa cctgcagcgg atggcggagg cgggcttcct cggccggcac
      781 ggcttctacg aggccatcga ctacacgccg caccggctgc cgccgaacgt cacgcacgcg
      841 gtggtgcgct cgttcatggc ccatcaccag ggcatggcct tcctggcgct ggcgcagacg
      901 cggctcggac cgctgatgca gcagcgcttc gccgccgaca aggcgctctg cgcgacgctg
      961 ctgctgctgc aggagcgcgt gccggccagt gccgcctacc gcagcagcga ggaggccgtc
     1021 ggcagcggcc cgaagccggc cgctgccggc accctgcccg agcgcccgaa ccgcagcttc
     1081 gataccgccg acacggcgct gccgcagctc gtcgtgctgt ccaacggcag cctgaacgtg
     1141 ctgctgacgc aggccggcgg cagccggctg gagcgccgcg ggctggcgct gacgcgctgg
     1201 cgcgaggacg gcacctgcga tgaccgcggg ctgttcttct acctgcgcga cacggcttcg
     1261 ggcgccgtgt ggtcggccgg tttccagccg gtgcgcgtgc ggccgccggt gttctcggcg
     1321 accttctcgg aagggcgcgc cgagttcctg cgccgcgacg gcgacatcga gctgcgcatg
     1381 gaggtcgtcg tctcgcccga ggacgacatc gagctgcgcc gcctgcacct gaccaaccgc
     1441 ggcacgagcg tgcgcagcat cgagctgacc agcttcgccg aggtggcgct ggcgccgccg
     1501 gccgccgatg ccgcgcatcc ggccttcagc aagctgttcg tgcagaccga ggccgtgccc
     1561 gaggcgcatg cgctgctcgc gacgcgccgg gcgcgcggcc acggcgaggc gcagccctgg
     1621 ctgctgcatg cgctgctgcc acggcccgag gacccgaaca cggcgctggc gccggcctcg
     1681 ttcgagaccg accgcgcccg cttcatcggc cgcacccgca gcagcggcgc cccgctcgca
     1741 ctcgccggcg cgccgggacc gctgtccggc accaccggcg ccgtgctcga cccggtgatc
     1801 gccatccgcc acgtgttcac gatccgcccc ggcgagtcga tgatcgtcga tgcgctgacc
     1861 ggcggggccg acagccgcga ggccgcgcag acgctgctgc agaagtaccg cgatccgcac
     1921 atggccgatc gcgtgttcga cctcgcgtgg atccacagcc aggtggtgct gcgccagctc
     1981 ggcgtcaccg agaccgacgc gcagctctac tgggagctgg cggcctgcgt ggtgttctcg
     2041 gacgcccacg gccgcgccga cgccgcgacc ctgcgccaga accggcgcgg acagtcgggg
     2101 ctctggagcc acgcgatctc gggcgacctg ccgatcgtgc tgctgcgcat ccaggacgtg
     2161 tcgaacctcg atctggcgcg gcagctgatc caggcgcacg cctactggcg cagcaagggc
     2221 ctggtcgccg atctggtgat cctgaacgag gaccacgcca gctaccggcc gctgctgctc
     2281 gaacagatca ccggcctgat cgccgccggc atcgaggcaa gctcgaccga caagcccggc
     2341 ggcatctacg tgcgcaacgc cgagcacgtc tcgcacgagg accgcgtgct gctggagtcg
     2401 gtggcgcgct tcatcgcctg ggaccgcaag ggcacgctgg agcagcagct gcgccgcagc
     2461 gccacgcagc cgctgctgcc gccggtgctg gcgccggccg ccgatgccgt ggcgccggcc
     2521 gcgccggtca gcctggtgcg cgaggacctg ctgctcgaca acggcttcgg cggctacacg
     2581 gccgacggcc gcgagtacct gatccgcctg cgccccgacg cgccgacgcc gctgccgtgg
     2641 gcgaacgtca tcgccaacgc cgaaggcttc ggcaccgtga tctccgaatg cggctcggcc
     2701 tacacctggg ccgtcaacgc ccacgagtac cgcctgacgc cgtggcacaa cgatccggtc
     2761 agcgacagct gcggcgaggc cttctacgtg cgcgacgagg acagcggccg ctactggtcg
     2821 ccgacgccgc tgccggcgcg cggccggggc gactacctct gccgccacgg cttcggctac
     2881 agcgtgttcg agcacatcga ggacggcatc gtcagcgagc tggcggtgtg ggtcggggcc
     2941 ggcctgccgg tgaagttctc gcgggtgatg ctgcgcaacg acagcggccg gccgcggcgg
     3001 ctgtcgctga ccggctacgt cgaatgggtg ctcggcgagc accgctggaa gtcggcaccg
     3061 cacgtgatca ccgaatgcga cgccatctgc ggcgcgctgc tcgcgcacaa cgcctacagc
     3121 agcgaattcc cgcacatgag tgccttcttc gacgccgacc ccgggccgca gccgggcgcg
     3181 cgcagctaca ccaccgaccg caccgagttc atcggccgca acggctcgct ggccgcgccg
     3241 gcggcgatgg ggcgcacgac gctcggcaac cgcaccggcg ccggtctcga cccgtgcgcg
     3301 gcgatccggc tgccgctgac gctcgccccc ggcgagcagg tggagctcgt gttccggctc
     3361 ggcgccgccc cgacccacga ggtcgcgcag cgggcggtgg cgatgcgcac gcccggcgtc
     3421 gcgtggcgcg aatggcgggc gatgcagttg cactgggagg cgctgctcga caccgtgcag
     3481 atcgacacgc ccgacccggc cgtcaacctg ctcggcaacg gctggctcgt ctatcaggtg
     3541 ctcggctgcc gcatgcaggc gcgcagcggc tactaccagt cgggcggcgc cttcggcctg
     3601 cgcgaccagc tgcaggacgc gatggcgctg gtgcacgccg cgccccagcg cctgcgcgcg
     3661 cagctcctgc tcgccgcctc gcgccagttc attgaaggcg acggccagca ctggtggcat
     3721 ccgccgggcg ggcgcggcgt gcgcacgcgc tgctcggacg attacctgtg gctgccgctc
     3781 gccacctgcc gctacgtcgg cgccaccggc gataccggcg tgctcgacga ggtgctgccc
     3841 ttcctcgaag gccggccggt gccggacggc gaggagtcct attacgacct gccgagcgat
     3901 tcgcagcagg ccgccagcct ctacgagcac tgccggcgcg cgatccgcac cggcctgcgc
     3961 tacggcgggc acggcctgcc gctgatgctg gccggcgact ggaacgacgg catgaacctc
     4021 gtcggcatcg acggccgcgg cgagagcgtc tggctcggct tcttcctgta cacggtgctg
     4081 caggacttcg cgccgctggc ggcacgccgc ggcgatgacg agttcgccac gctgtgccgg
     4141 cgcgaggcgg ccacgctgcg cgacaacctc gaacgccacg cctgggacgg cgagtggtat
     4201 ctgcgcgcct ggttcgacga cggcagcccg ctcggctcgc aggccagcgc cgaatgccgc
     4261 atcgacgcca tcgcccagag ctgggcggtg ctctcgggcg cagccgatcc ggcgcgctcg
     4321 gcgcaggcca tggactcgct cgaacgccgg ctggtgcgcc gcgaggccgg catcatccag
     4381 ctcttcgacc cgcccttcag cggccacggc ccgctcgatc cgggctacat ccgcggctat
     4441 ctgccgggcg tgcgcgagaa cggcggccag tacacgcaca gcgccgtgtg ggcggtgatg
     4501 gccttcgccg cgctcggcga gcgccagcgc gcctgggagc tgttcgacct gatcaacccg
     4561 ctgaaccgca cccgcgacgc cgccggcatc gaacgctacc gcgccgagcc ctacgtgatg
     4621 gcggccgatg tctatggcgt cgagccgcac accggccgcg gcggctggac ctggtacacg
     4681 ggctcggccg gctggatgta ccggctgctg ctcgaatccc tgctcggact gacgcgccac
     4741 gccgatgcgc tgcggctcga tccgctgctg ccggacggct ggccgggcta cacgctcacc
     4801 tatcgccacg gcgccagccg ctggcacatc gaggtcagcc gtggccgtgg cggtgagggt
     4861 gcgcagctga gcgtcgacgg gcgcgtgcgc agcgacggcc gcatcccgct cgtcgatgac
     4921 ggcgccgagc atcgcgtcga gctgaagctc gacgggcggc agcggcaggc ggcgtgagcg
     4981 gcggtcacgg acggccggca ggccgggata tgcccgccgc ggccgcccgc gcgcagctca
     5041 tgtcccggac ggggccacgt gcagcccgaa gccgctggtg ccgggggctt ccagccccgg
     5101 gcgcagccgc ccgcgtgcgg cggctgatcg aatcgctggc cgggcaggtc ggcggcgtgg
     5161 caggcatcgg gccggtgcgc ccgccctccg gggcgcagcc gacgggcgga tgaaaccgcg
     5221 gccgtccggc cggcgcgcag cggtagtgaa agattgtcag gcgccggtgg ccgtcccggg
     5281 cattcttcga gaatgcgcgc gcgttccccc cggcgcaggg cggcgcggca gcgccgcggt
     5341 ccgcctcgct tgcacatcca ccatcagtca ctgcccgcac cggccgccgc cggggcaggc
     5401 cgggctgcgt ttgcagaatg cgtttgcaga aggggagtcg gaaaatcatg ttcagaggca
     5461 ttctcggggc ggcgctgctg gcggccgtga ccttcgtgct gccggcccgg gcgggcgatc
     5521 aggatttcac gctggtcaac cgcacgggcg tcgaaatcca caaggtctat gcgtccccgc
     5581 acagttcgga cgactgggag gaggacatcc tcggggacga cacgctgccg gatggcgaaa
     5641 gcgtcaacat caccttcagc ccgaaggagc gggccgcgat gtgggatctg cgcgtcgagg
     5701 acagtgacgg caacttcatc acctgggaga gcctcgacct gctgcagatc tcgcgggtca
     5761 cgctgcacta caagaaaggc aaggtgtggg cgacgctcga ctgagcgccc gctggccgcg
     5821 gctcagccgt cgaggtcgag ccggcggtac tcgccggcga gccgcgcact cagccgcggc
     5881 agccgccgca ggtagtgcag caccaccgca agcgccccga gcccggccag cgccagcgtc
     5941 gcctgcacgc cgaagtgccc ggcgagcagg ccggacatca gggcgccgag cgggaagacg
     6001 ccggcaaacg ccagcacgta caggctcatc gcccggccgc gcaggcgcgg ctcgacgacg
     6061 gtctgcagca ggctgttgag cgcggcggcc gtcgtcatca tgccgccgcc gatcagcatc
     6121 atcatcccga gcgccagcgc atacggcggt tcggctgcga gcccgagcag accggtggcg
     6181 gccgcgagcg cgctggcggc ggcgatgcgt tcgtagccgc tgctgcgctc gcgcgccgcc
     6241 agcgccagcg tgccggcgat ggcgccggca ccggccgccg acagcagcca gccgagcgtg
     6301 tgcgggccgc cgccgtagac ctcgcgcgcc acgaccggca tcagcgtcgc gtacgggccg
     6361 acggcgaggc tcagcggcgc cagcagcccg agcgccgcgg ccagcggtgc gaagtcgcgg
     6421 gcatagcgca ggccttcgcg ccagtgttcg gcgaacggca ccggcggcgc ccgcgggggc
     6481 ggaggcggcc agtcgatgcg cgcgagcgcc gcgagcacgg cgacgtagct cagcgcattc
     6541 agcgcgaagc acacgccttc gccgagcgcg gcgaccagcg cgccggcgag cgccgggccg
     6601 agcgcgcgcg cgacattgaa gctgagggaa ttcagcgcga tggcattcgg cagcagcgtg
     6661 cggtcaccga ccagctcggc ctgcagggcc tgacgcagcg gcaggtcgag cgccgagatc
     6721 gcgccgagcg cgagtgcgag cgcgaccaga tgccagacct cgatgcgacc ggcgagcgtc
     6781 agcgccgcga gcccgcccgc ctgcacggcc gcgaggcact gcgtcgtcca cagcgcacgg
     6841 cgccggtcga tgcgatcgac cagcacgccg gcgaacgggc cgagcacgag cacgccggcg
     6901 ttggcgcaga agccgatcag cccgagcagc gccggcgagc cgctcaggcg ccaggcgagc
     6961 cagcccattg ccacctgctg cagccaggtg ccgagcagcg acacgctctg gccgagccag
     7021 tagcggcggt agttggcgct cgcgagcgca cggaaggggt tgcgcatggc ggatcggggg
     7081 tcggggaggc ggcggagggc ggctgtatgg cgcaggggtg cgcaagcgca gcggactgtg
     7141 acgccggcat gacgattctg tcatgtaccg ctacgctgcg cgggctgtcc tgcaaaccag
     7201 caggccgcag gagaccacga tggcactgcc gcccgatgcg cgccgccccg gcgcggatgt
     7261 gctggctgat cccgccggtt atttcgaaga tctggaacgg gtcgctgccg gcagcacggc
     7321 gctgccggcg ctgcgctgcc tgctcgtgac cgcggcgctg cgcgacgacg acaccggccg
     7381 gcatacggtg cgcgtcggct acatggccga gctgatcggc cgccggctcg ggctggacga
     7441 ggacacctgc ctgcgcctgc gcgccagcgc gccgctgcac gacatcggca agctcgcgat
     7501 cccggatgcg atcctgaaca agccggggcc gctcgatgcc accgagatgg cggtgatgcg
     7561 ccgccatgcc gagttcggcg agcgcatcct cgcgagcacc ggcgagccgc tgttccggat
     7621 ggcggccgac atcgccggcg cgcaccacga gcactacgac ggcagcggtt acccgcgcgg
     7681 tctcaggcgg gacgcgatcc cgctcgcggc gcgcatcacc gctgtctgcg acttctacga
     7741 tgcgctgcgc atggaccggg tctaccgcaa ggcgctgccg gatgcgaccg tgctcggctg
     7801 gatgcaggag cagcgcggcc gggccttcga tccgcaggtg ctcgatgcct tctccgactg
     7861 ctgcggcgaa ctcgccgagg cccacgactg gctggaggag caggcggcgc ggctgttcac
     7921 gatgctgcgc gcgcaggccg ccgggcagtc gccgccggtc tgagggcgtc gatgtacggc
     7981 tggcgcccct cagccgcgcc cgatcggctc gacgctgacg cgcgccgcgc cgctgcgcat
     8041 gccgatgcgg cgggcggcgg ccggtgacag gtcgatgatg cggccgcgga agccgacgcc
     8101 gcggtcgttg atcgtgacca cgacgctgtg gccggtgtcg agccgggtca cgcgcaccgt
     8161 ggtgccgagc ggcagcgtgc ggtgcgcggc ggtcatcgcc tcggggtcga agcgttcgcc
     8221 gctggcggtg tggcggccgc tgaggcgccg gctgtagacg gtggccttgc cgaggctggt
     8281 cgaggcggtc gccgccggtt tcgtgcgccg cgtcgcgacg gcgtggcggc cgggcgcggg
     8341 cttgcgggcg gtgctgcggg cggaggcgtg cgccgcctgg cggctgccgg tgcgggcttc
     8401 ggcgtgccgg ggggcgggct tgccggcgtg cgtgacgtga ggcgtggcgg tggctttcgc
     8461 cggggttttc ggccgggccg gggccgcggt cgagacggcg agcagcagcg cggcggcgag
     8521 ggcctgcgtg cgcaggcggt ggcggtacgg actcatcgcg aaccctccgg aagcagcccg
     8581 gccgtgatgg caggccgata ccgccattat gagggcggca ccggccgatc ccgagacccc
     8641 gcgttcacgt catccggccc gcgtcatccg gctcacgaca tcggcggttc ggtctgctgc
     8701 agccggtgca ggccggcgta gacgccgccg gcggcgagca gcgtcgcgtg cgtgccggtc
     8761 tcgacgatgc ggcccttggc gagcacgacg atgcggtcgg ccgattcaat cgtcgagagc
     8821 cggtgcgcga tcacgaaggc ggtgcgcccg gcgcgcaggc gttcgagcgc ctgctgcacg
     8881 aggcgctcgc tctgcgtgtc gagcgccgag gttgcctcgt cgaggatcag gatcggcgcg
     8941 ttcttcagca gcgcgcgcgc gatcgccagc cgctggcgct ggccgccgga cagccgcatg
     9001 ccgcgctcgc cgatgacggt gtcgagcccg gccggcagct cgcggatgaa ctccagcgca
     9061 tgcgcggcct cggcggcggc ctcgatgtcg gcgcgcgagg gcggcgtgcc gtggccgtag
     9121 gcgatgttgg cggcgaccgt gtcgttgaac agcaccgtct cctgcgacac gtaggcgatg
     9181 ttggcgcgca gcgacgcgag cgtcagctcg cggatgtcgg tgccatcgag cgtgatgcgt
     9241 ccggccgaaa gctcgtagag ccgcggcagc agcgccatca gcgtcgtctt gccgctgccc
     9301 gaggcaccga ccagcgcgat ggtctcgccg gggtcgaccg cgagatcgat gccgtcgacg
     9361 acgccggccc cgttgcggtc gtaggcgaag cgcacgccct cgaagcgcac cgcgccttgc
     9421 gcccggccga tcgtgcgcgt gccggtgtcg gcctcgggct cggcatcgag cagcgcgaag
     9481 atgctctcgg ccgcggcgag gccgcgctgc aactggtcgt tgaccttggt caggcgcttg
     9541 atcggcgcga acatcatcgc catcgcgccg aacagcgaca cgaagccgcc gaccgtgatg
     9601 tcgccttcga gcgattcgag cgaggcgagg tagatcaccg ccgccagcgc cagcacgccg
     9661 aacagctgca cgccggggat cgccgcgttc gagctggtgg cgagcttcat ctgcagccgg
     9721 cgcacccagt tgctggtctt gaagaagcgc gccgactcgt agtcctgacc gccgtagatc
     9781 ttgacgacct tgtggccgct cagggcctct tcgagcacgc ggttgacctc gccgacctcg
     9841 cgctgcagcc gccgcgacag cagccgcagc cgcttcgaga ccagacgcat gaccagcgcg
     9901 atcaccggca cgatcgccag cgtcagcagc gacagcttcc agttcaggta catcatccag
     9961 cacagcaggc cgatgacggt caggccgtcc ttgaccatcg tcaccagcgc atcggtgcag
    10021 gcgttcatga cctggttggt ttcgtagctg acgcgcgaca gcagcgtcgc cgtggcgtgg
    10081 ccgtcgtaga acgcggtcgg catctgcagc agcttgccga acagcgcctg acgcaggtcc
    10141 atgaccacgc gcgtcgcgac ccacttcatc gccacgtcgc tgacgaaatc ggcggcgctg
    10201 cgcaccagga acagcccgac cagcatcagc ggcaccgtgc gcatcagctc cgggtccttc
    10261 tgcacgaagc tgccgtcgag cagcggcttc agcagcgcgg cgatgcccgg ttcgcagagc
    10321 gcgagcacga cggtgccggc cagcgacagc gcgaacatgc gcgcgtgcgg ccgcacgaag
    10381 gtcagcaggc gcagatagag ttcgcggctg gtcatgggct tgcgggggcg ggggcagggc
    10441 cgcgcatggt aacggcgccg gcgccgccag cgttagcgcg acggccgggg cgggcatgcc
    10501 gctgtgctat cctcccgctt ccttcggacc ctggtgtcgc cgtgtgcgcg ccaacgacta
    10561 caaacggcag acccgctgca tgccggcgtc ccggaatccc gtctcccgcc catgagttcc
    10621 tccaccgtcc acgtcgatgt gctgttcgtc ggcggcggca tcgcagggct gtggctgctc
    10681 aactgcgtgc gccaggccgg ctacagcgcg ctgctggtcg aatccgaacg catcggtgcc
    10741 ggccagtccg gggccagcca gggcttcgtc gtgcaccggc cgcgcaccgg cgcgaccgtg
    10801 ccgaccgaca aggaagccgc ggagctcgcg agcgtcgtcg ggcgctggcg cgaactgctc
    10861 gccggccgcg cggtgccgga cctctcgggc gtcagcgtgc tgagcgagca tccgctgcgc
    10921 tggaccctgc ccgggcagcc cgggcgcagc ctgctcgatc gcctgctgcg ccgttcgccg
    10981 ccgctgccgg atgccgctgc cgagaccggt ttcgacatcc ccggcgtgct gcgccatccg
    11041 atgcgccgcg gcagcctgtc gacgcaggaa ggcttcatcg tcgacaaccg ctcgctgctc
    11101 ggtgcgctgc tcgatccggt gcgtgatgcg ctggtgcaca cgcagggcgc ggcggtcatg
    11161 gcccgcgacg gcggtttcaa cctgcgcatc gaggagcgcg agccggtggt cgtgcgtgcc
    11221 cggcgcacgg tctataccac cggcgtcggc gcgaacctgc tcggcagcgt gtcgctgcag
    11281 gtctggcatc agcagatggt catgatccgc ggcgagggac tggccgatga cctctacatc
    11341 aatgccgtca acgaggccgg ccggcggctg ttcaacatca gttcccaccg cgacctcacg
    11401 ggtcggccgg tctggtacta cggcggcgcg atggtcgagt ccggcgagca ggtcgatgcc
    11461 gcccggcaga tccgcaactg ccggcgcgat ctcggcgcgt atttcccgtg gatggcgctg
    11521 agcggcctcg aattcgccgc gcaccgcgtc gatcgcgccg ccgtgcgcat gcgcaacggt
    11581 ctggcgctgc tgccgcagct gcgcgtcgag gaccagcgcg tggtcgcgtg gccgaccaac
    11641 ctgtcgacga tgccgctgct cggcgaccgc atgctggcga tgctcaggcg cgagggcatc
    11701 gagccgcggc cggccgacct gcatctgctc gccgactggc cgcagccggc gctggccccg
    11761 gcgccgtggg acgaagaggc ccagacatgg cagagctgaa cgcccgtgcc gcgacgcccg
    11821 cgcccagccg gcgcgagcgg ctggcccggc ggctgtggtc cgggctggtc gcggcgctgg
    11881 tgccgctcgc gctggcgcgc ctctggtggc gcggccggcg cagtcccggc tatcgcgagc
    11941 gcttcgccga gcgtttcggc gcggcaccgc ggctcgcgcc cggcgcgatc tggctgcacg
    12001 cggtctccct cggcgagacg cgcgcggcgc tgccgctgat ccgggcgctg ctcgcgcgcc
    12061 accccgaccg gccgctgctg gtgacgacga cgacgccgac cggctcggcg caggtgcgtg
    12121 aggcgctcgg cgagcgcgtc gcgcactgct acgcgccctg ggacctgccc ggggcctgcg
    12181 cgcgctttct cgaccgtgcg cagccggcgc tcgccgtgat catggaaacc gaactctggc
    12241 cgaacctgtt cgccgcctgc gcggcgcgcg gcgtgccgct cgtggtcgcc aacgcccggc
    12301 tgtcggcgcg ctcggcgcgc ggctatgcgc gcgtgccgtg gctgatccgc ccgacgctgg
    12361 cggcgacgac gctgatcgcc gcgcaggccg aggccgacgc cgggcgcttc gtcgcgctcg
    12421 gggcgccgcg cgtcggggtc agcggcaacc tgaagttcga tctggcgctg cccgaagcac
    12481 tgccggcgcg ggcgcaggcg ctgcgccggc actggggcgc ggcgcggccg gtgtggatcg
    12541 gcgccagcac gcacgccggc gaggacgaac tgctgctcga tgcgcatgcg cggctgcgcg
    12601 agcgctggcc cgagctgctg ctgctgctgg tgccgcgcca tcccgagcgc ttcgatgcgg
    12661 tcgccgccct cgtgcaggca cgcggcttcg cgctggcgcg gcgctcgcac ggcgagacgg
    12721 ggcaggcggc ggccgtatac ctcggcgaca cgatgggcga gctgctgctg ctcgcggcgg
    12781 cgagcgatgc ggccttcgtc ggcggcagcc tggtgccgac cggcggccac aacgtgctgg
    12841 agccggccgc gctcggcgtg ccggtcgcct gggggccgca catgttcaac ttcgccgccg
    12901 ccgagcagct gctcgccggg gccggggccg gccggcaggt cagcgatgcc ggagccctcg
    12961 tgcagaccct cgccgactgg ctggccgatc cggcgctgcg cgcgcgctgc ggcgcggccg
    13021 gggccgccgt ggtcgcggcc aatcgcggtg cgctggcgcg gctgctcgaa cggctcgacg
    13081 cgctgctgcc gccggccgcg gctccctgaa tccgttgaat ccgttgcata ggcccttccg
    13141 catgttgcgc tgcagcgccg ggcggggtca cagtgcgtgc cggtgctggc cgttgcgccg
    13201 caactcctta taatcgcgcc cctttacgct tgcggagtgt cgctgaagat gtctggattt
    13261 ccccgcttcc cgcgtcgctg gctcgcgggc ctcgcgctcg ccgtcacatc cgcgctcgtg
    13321 cacgccgccg acacctggcc gctgccgccg gccggctccc agctgatcgg cgagacccgc
    13381 atcgaatacg cgaagcagga ggacaccttc atcgacatcg cgcagcgcaa cgggctcggc
    13441 tacgacgaga tcgtcgccgc caaccccggc gtcgaccgct ggctgccggg cgagggcacg
    13501 ccggtgatcc tgccgctgca gcacatcctg ccggacgcgc cgcgcgaggg catcgtcgtc
    13561 aacctgccgg agatgcggct gtactacttc ccgcgcggcc agaacatcgt gaagtcctac
    13621 gcgatcagca tcgggcgcat ggactggcac tcgccgctcg ggcgcacgct cgtcaccgcc
    13681 aaggtcgtga acccgacctg gacgccgccg gcctcgatca aggccgagca cctcgccaag
    13741 ggcgatccgc tgcccgatgt cgtgcccggc ggtccggaca acccgctcgg cacgcgcgcg
    13801 atgcgcctcg gcatccccgg ctacctgatc cacggcacca acaacccgaa cggcatcggc
    13861 atgcgcgtca cgcacggctg catccgcatg tatccggacg acatcgagcg cctgttcccg
    13921 gatgtgccgg tcaagacgcc ggtgacgctg gtcaaccaga ccgccaagct cggctggcag
    13981 aacggcgtgc tgtacctcga ataccactcg ccgctcgaag aggacgagat tccgacccag
    14041 cagcagatcg acgagaccgt gcagctgatc cattccaaga tcggcgaccg cgtgccgctc
    14101 gtgtccccgg acatcgtgca gaccgtcgtg cggcaggcga gcggcatgcc ggtggcgatc
    14161 tcgctgccgg tcgcgatcga cccctcgcag ctgccgccgc cggcgagcga gccggtgccg
    14221 ccg
//
LOCUS       DAOQIE010000054        14165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2055_length_14165_cov_7.713466, whole genome shotgun sequence.
ACCESSION   DAOQIE010000054 DAOQIE010000000
VERSION     DAOQIE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_14165_cov_7.713466"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PK144_05225"
     CDS             complement(<1..928)
                     /locus_tag="PK144_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNF65160.1"
                     /translation="MRMDKFTSKFQMALSDAQSLAVGRDHQFIEPLHLMAALIDQQDG
                     TVRPLLAQTGANVQTLRAQIGEALDRLPRVEGHGGDVQLARELVNLLNLTDRLAQQRR
                     DQYISSELFVLAAVEDKGTLGEILRRHGASKPLIEKAVEQLRGGQKVDDPNAEDSRKA
                     LEKYTIDLTARAEAGKLDPVIGRDDEIRRVTQVLARRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGEVPEGIKGKRVLSLDMGALIAGAKFRGEFEERLKAVLNDLAKQEGQIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELH"
     gene            complement(998..1618)
                     /locus_tag="PK144_05230"
     CDS             complement(998..1618)
                     /locus_tag="PK144_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015673360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF65161.1"
                     /translation="MPFSVPVLPASGPRVQLRGWRDDDLDAFAALDADAEAGYWLGGP
                     FDRAASRARMAAIRARLDGQGFGFWALELPGVAPFIGCAGLNRPGFAAPFLPAVEIGW
                     RLARPYQRRGLALEAARQVLAHAFGPLGLPGVVAFTAIGNVRSRALMVRLGMQHDPDG
                     DFDHPQLPAGHPLRRHVLYRLARDARPACAGAGPPAAGSAFLVTPP"
     gene            1748..2767
                     /locus_tag="PK144_05235"
     CDS             1748..2767
                     /locus_tag="PK144_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNF65162.1"
                     /translation="MDSVTQIALGAALGVAVLGRQGRVWRAVLTGAVVATLPDLDAFI
                     DHGDAIRNMTLHRAESHALFWLTLASIPLTALVVAIWREPGLWRRWWLAVWLMLLTHP
                     LLDTMTIYGTQLALPWSDEPWGIGSVFIIDPLYTLALLIGTGVALVRRADARGRRWNA
                     AGLALSTLYLGWGLGAQQLALARAEAALAAAGQPAVQVLALPTAFNSLLWRLVVITPD
                     DGWLEGYTALPDAGLPRFERFPRGEALFARWRHDPAVARLARFTHGYWQMRRLADGRV
                     VITDLRMGQQPWFTFEFVVGRDLGAHREPAPATRIREPPPVATVLRWIVHRIGDESLP
                     PPRLP"
     gene            complement(2776..3258)
                     /locus_tag="PK144_05240"
     CDS             complement(2776..3258)
                     /locus_tag="PK144_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNF65163.1"
                     /translation="MDPDLALQLATLIRRQRWAALATLGPDGPEASHVAYAIEGAFDG
                     VLLHLSQLAAHTKQLLADPRASLAIGEPDDGRADPQTLARVTLSGMVEPLARETEDYL
                     AAQARYLARLPESAQLFGFGDFRLFRLRPQRARLVGGFARARTFDAGALQAAGALRLP
                     "
     gene            complement(3302..4048)
                     /gene="pgeF"
                     /locus_tag="PK144_05245"
     CDS             complement(3302..4048)
                     /gene="pgeF"
                     /locus_tag="PK144_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011535713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNF65164.1"
                     /translation="MSGEPLALLQPDWPAPANVRAAATTRRGGVSRGPYAALNLGDHV
                     GDDPAAVAENRRLLSAALGLARAPAWLEQVHGCAVAAAETVHAPVRADAAITRTPGVA
                     CVVMTADCLPVLFCDRGGRVVAAAHAGWRGLAGGVLEATLARMAVDPAEVLAWLGPAI
                     GPAAFEVGPEVRAAFVADDPGAADAFVPARADRLLADLYALARRRLMRAGVGQVSGGG
                     RCTYSEADTFHSYRRAPVSGRQATLVWLSA"
     gene            complement(4045..5058)
                     /gene="rluD"
                     /locus_tag="PK144_05250"
     CDS             complement(4045..5058)
                     /gene="rluD"
                     /locus_tag="PK144_05250"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNF65165.1"
                     /translation="MPEFERIDCLIPPECAGQRLDQALAALLPDHSRARLQQWLKDGT
                     LTVDGRPAKPRALVLGGERVSGAYERTEVIADRPQDIPLAVRYEDEDILVIDKPAGLV
                     VHPAAGNPDGTLVNALLHHAPVTAQLPRAGIVHRLDKDTSGLLVVAKSLRAHTALVEQ
                     LQARTMGREYQAIACGVLVSGGLVDVPIARHGTDRKRMAATPGGKPAITHFRVRERFR
                     AHTHLALKLETGRTHQIRVHMAFMHHPLLGDPVYGGRLRLPPGCSDELATALKGFRRQ
                     ALHARRLTLTHPGSGRRLSWSAPTPPDLAALLDVLRRDAGLDGLDARAGVDADGVEWE
                     YVE"
     gene            5167..5973
                     /locus_tag="PK144_05255"
     CDS             5167..5973
                     /locus_tag="PK144_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNF65166.1"
                     /translation="MSARFLRLRLATVLAAALLAPGCSWLSEKADETRDWSAAKLYSE
                     AKDALNNGDYTKAIGYYDKLQARYPFGRYAQQAQLESVYAYYRDGEPDSAVAAADRFI
                     KTYPRHPYVDYVYYLKGLTNYNRASGPLDRLMPPDPTRTDASAALQSFTDFNDLVTKF
                     PDSRYAPDARARMLFLRNNLASYELNVARHYQSRGAHVAAVNRAKYVVENYASSPAAT
                     EALVIMVRSYTAMGLPELARDALRVLQKNDPQNPDIPKLTTLVGTAPGKG"
     gene            5992..6513
                     /locus_tag="PK144_05260"
     CDS             5992..6513
                     /locus_tag="PK144_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNF65167.1"
                     /translation="MWDFFETVRHRHSVRIYQQDMPVDEARLHAILEMACAAPSAGDL
                     QAYRIVVVRDADERRALSVAADNQGFIAEAPVCLVFCADPVRSGAEFGERGERLYALQ
                     DTTIAAAYAQLAIVAAGMGSAWIGYFDDQAVRDLLGLDPALQPVALMTVGYPAELPVP
                     TPRRRLAEVVSFR"
     gene            complement(6539..7177)
                     /locus_tag="PK144_05265"
     CDS             complement(6539..7177)
                     /locus_tag="PK144_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF65168.1"
                     /translation="MRYAVAALWLLLGAAAHADSSAPLLTRAQALLAEARANPGVQAD
                     LLERGRERSAFCAACHGAGGESTRADYPDLAGQSIEYLLTQFGQFQGAERYRKVMNDL
                     MAKLTDDEKALLAVYYALQPPQPEPAADAALVASGAKLYAARCQSCHGADAHGKDGRA
                     RLAGQKPGYLLRVLNDFRQGHDRSPDSVMRSMVQGLSEADVQALASFLGAQP"
     gene            complement(7297..8943)
                     /locus_tag="PK144_05270"
     CDS             complement(7297..8943)
                     /locus_tag="PK144_05270"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNF65169.1"
                     /translation="MSAAAPAALTVAMAQFNPLVGDIAGNTRRILELARQAQTELGAG
                     VVVFPELALCGYPPEDLLLRPEFVSRCEAALAELATAAAHGPALVIGHPQRGLGGLYN
                     AASFLAGGRALGVYAKQLLPNYGVFDEKRYFVPGSGPCVVEHLGTRFGLTVCEDIWQP
                     GPVAQAAQAGAELILNLNASPWHRAKAGERLTILHARVHEAGVPIVYVNQVGGQDELV
                     FDGGSLLVDADGTLLARAPAFVEGLYPLGFDRIDGALVPRPGPLAEAPEDEAEVYAAL
                     VLGTRDYVRKNGFRGAVLGLSGGIDSALTLAIAVDALGADAVSAVSLPSRYTAGMSNE
                     DAAIEARTLGVDYHLLPIEPAFDAFLHTLEPLFAGLPPDTTEENIQARCRGVLLMALS
                     NKTGRLVLTTSNKSETAVGYSTLYGDMAGGFAPIKDVPKTLVYRLAAWRNRQAQAPVI
                     PQRVIDRPPSAELRPDQTDQDSLPPYDVLDAILEAYVERDRSVEELVADGFERATVER
                     VARLVILNEYKRRQAAPGVRITPRAFGRDRRYPITSGFRR"
     gene            complement(9104..9976)
                     /gene="sucD"
                     /locus_tag="PK144_05275"
     CDS             complement(9104..9976)
                     /gene="sucD"
                     /locus_tag="PK144_05275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNF65170.1"
                     /translation="MSILIDKNTKLICQGFTGSQGTFHSEQCLAYGTNLVGGVSPGKG
                     GSAHLGKPVFNTVHEAVRDAGANASMIYVPAPFAADAILEAADAGIKVIACITEGIPV
                     LDMLKVKAALKGSDVRLIGPNCPGLITPGECKMGIMPGHIHQPGKIGIVSRSGTLTYE
                     AVHQTTRIGLGQSTCVGIGGDPIHGMSFIDVLEMFEADSATKGIVMVGEIGGSAEEEA
                     AEFIKASVRKPVVAYIAGVTAPPGKRMGHAGAIIAGGKGTAKDKFAALEAAGVKTVRS
                     PADIGEAINGFFAG"
     gene            complement(9990..11150)
                     /gene="sucC"
                     /locus_tag="PK144_05280"
     CDS             complement(9990..11150)
                     /gene="sucC"
                     /locus_tag="PK144_05280"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNF65171.1"
                     /translation="MNLHEYQAKELFRQFGVAVPRGIKATSPKEAVAAAKELGGSVWV
                     VKAQVHAGGRGKAGGVKLARSLQEVEDIAGSLLGTRLATKQTGPEGLPIHALLIEEGL
                     NIVDELYLSVLVDRSTKRIAFIGSSEGGMDIEEVAESTPEKILTVAIDPAAGFQAYEA
                     RQLGFGMGLAKPQVDHLVKVMNGLYDMFVAKDASLVEINPLIVTGEGKLLALDAKIGL
                     DDNALYRHKDIAEWRDSTQEDERENLAHEIGLNYVALDGNIGCMVNGAGLAMATMDMV
                     KLHGGEPANFLDVGGGATAERVAKAFKLILSSDKVRAIFVNIFGGIVRCDLIAEGIIA
                     AVKEVGLTIPVVVRLQGTNVEAGRKMLSESGMNIIAADDLTEAAKAAVAAAK"
     gene            11253..11507
                     /locus_tag="PK144_05285"
     CDS             11253..11507
                     /locus_tag="PK144_05285"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNF65172.1"
                     /translation="MSRYLLLLLLVLAAVWWLRRARRPQAPARPQAPAQPAKPDWPAM
                     VRCTECGIHFPATDAVRDADARPYCCPAHRERHALRPPKD"
     gene            11578..13278
                     /locus_tag="PK144_05290"
     CDS             11578..13278
                     /locus_tag="PK144_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF65173.1"
                     /translation="MDRRRGWSVLRAYVLYRLVLATLLLGITLADLAGKLFGAEDPRL
                     YWWTIGGYFGLLATGFLLSYLRRPDLIVQATLHAGLDIVVFTALMYASGGASGSLGAL
                     LVSAIAGSAILLPRRLVITVTAAAALAIIGVWLYGIWQSFALRTGAPYLSLDLLPPLV
                     DWALVHGASGGQTALICLALAAAAAAAYTIAERTRNSETLVLERTQELLEMAELNAAI
                     IRHLQSGILVVDRHARVKLMNQMAAELLDFHERPIGLPLSDISPLLSQRLGAWIGSGL
                     TQTQPFRPAEHLPELIPEFTALSGNLAFETLVFIEDSNAAAARLQQIKLAALGRLTAS
                     IAHEIRNPLASISHAAQLLVESTANPGEKRLGKIIHDNAGRANQIIANVLDLSRRDKA
                     SPEDIHLRSWLEDFCHDFMRGRGDPMPEMEIRVQPEDLTVHFDAGHLQQVLWNLCNNA
                     CEHGTLPGESPRVRILAALDPERFRPYLDVIDFGAGIPEAEARQIFEPFFTTNPKGTG
                     LGLYLSREMCEANRAQLQYRPNPSGQGSCFRLTLPTATRTPATTMNTTEEPTWNLAGR
                     "
     gene            13257..>14165
                     /locus_tag="PK144_05295"
     CDS             13257..>14165
                     /locus_tag="PK144_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF65174.1"
                     /translation="MESCRALILDDEPDLRDLIALTLEPMGVQCVPAATLSAARTALA
                     SQGPFHFCLTDMRLPDGNGLDLVALIQRQYPEMPVAVITAHGNVESAVEALKLGAFDF
                     VSKPFDIATLRAMVDQARRLAGVPAGRDGGPNRPDLIGASPAMERVRSMIRKLARSQA
                     PVFIGGESGTGKEVVARLIHASGSRAERPFVPVNCGAIPAELMESEFFGHRKGAFTGA
                     VADKPGLFQAAHGGTLFLDEVAELPQAMQVKLLRAIQERAIRPIGGAHELPVDVRVLS
                     ASHRDLAAEVQAGRFRQDFYYRLNVIE"
BASE COUNT         2032 a         5120 c         4924 g         2089 t
ORIGIN      
        1 agtgcagctc gccgcgcgcc agcgccggct tgagcatgtt gccggcatcc atcgcgcctt
       61 cggccttgcc ggcgccgacc atcgtgtgca gctcgtcgat gaacaggatg atctggcctt
      121 cctgcttggc gagatcgttg agcacggcct tcagccgctc ctcgaactcg ccgcggaact
      181 tggcgccggc gatcagcgcg cccatgtcga gcgacagcac gcgcttgccc ttgatgcctt
      241 ccggcacctc gccgttgacg atgcgcagcg ccagcccctc gacgatcgcg gtcttgccga
      301 cgccgggctc gccgatcagc accggattgt tcttggtgcg tcgcgccagc acctgcgtga
      361 cgcggcggat ctcgtcatcg cgcccgatca ccggatcgag cttgccggcc tcggcgcgcg
      421 cggtcaggtc gatcgtgtac ttctccagcg ccttgcggct gtcctcggcg ttcgggtcat
      481 cgaccttctg gccgccgcgc agctgctcga ccgccttctc gatcagcggc ttgctggcgc
      541 cgtggcggcg caggatctcg ccgagcgtgc ccttgtcctc gacggcggcg agcacgaaca
      601 gctccgagga gatgtactgg tcgcggcgct gctgcgcgag ccggtcggtg aggttcagca
      661 ggttgaccag ctcgcgcgcg agctgcacgt cgccgccgtg gccctcgacg cgcggcagcc
      721 ggtcgagcgc ctcgccgatc tgcgcgcgca gggtctggac gttggcgccg gtctgcgcga
      781 gcagcggccg gaccgtgccg tcctgctggt cgatcagcgc cgccatcagg tgcagcggct
      841 cgatgaactg gtgatcgcgt ccgacggcga gcgactgcgc gtcgctcagc gccatctgga
      901 acttgctggt gaacttgtcc atgcgcatgt gcggattccc ccggggcggg tgtgggatgc
      961 gggtctgctc ccggaaatga ggggatggac cgcggaatca aggcggtgtc acgagaaatg
     1021 ccgatccggc cgccggcgga ccggcaccgg cgcaggccgg gcgggcgtca cgggccagcc
     1081 ggtacagcac gtgccggcgc agcggatgcc cggccggcag ctgcggatga tcgaagtcgc
     1141 cgtccgggtc gtgctgcatg ccgagccgca ccatcagcgc ccgggagcgc acattgccga
     1201 tggccgtgaa cgcgacgacc ccgggcagcc ccagcggtcc gaacgcatgc gcgagcacct
     1261 gccgggccgc ctccagcgcc agcccgcgcc gctggtacgg ccgggccagc cgccagccga
     1321 tctcgaccgc aggcaggaag ggagcggcga agcccggccg gttcaggccg gcgcagccga
     1381 tgaacggcgc gactccgggc agctccagcg cccagaagcc gaagccctgc ccgtccagcc
     1441 gcgcccggat cgccgccatc cgggcgcggc tcgcggcgcg atcgaagggt ccgccgagcc
     1501 agtagccggc ttcggcatcg gcgtcgagcg ccgcaaacgc atcgagatca tcatcccgcc
     1561 agccgcgcag ctgcacgcgc gggccggacg ccggcaggac cggtacggaa aatggcatgg
     1621 tctgcctccc cgatcccgcc ctcccgggcg gagcgcctgt accaccggca acagccgggc
     1681 ccggtgaaca ccccgcgccc gtctgcagtc gattccgttt tccctgtcca cgagcctgcc
     1741 gccgccgatg gattccgtca cccagatcgc ccttggtgct gccctcgggg tcgccgtgct
     1801 cggccggcag ggccgcgtct ggcgcgccgt gctgaccggt gccgtcgtcg cgacgctgcc
     1861 cgatctcgat gccttcatcg atcacggcga tgcgatccgc aacatgacgc tgcaccgggc
     1921 cgaaagtcac gcgctgttct ggctgacgct ggcgtcgatc ccgctcacgg cgctggtggt
     1981 cgccatctgg cgcgagcccg ggctctggcg gcgctggtgg ctggccgtgt ggctgatgct
     2041 gctcacgcat ccgctgctcg acacgatgac gatctacggc acgcagctcg cgctgccgtg
     2101 gagcgacgag ccctggggca tcggcagcgt gttcatcatc gacccgctgt acacgctggc
     2161 gctgctgatc ggcaccggcg tcgcactcgt gcgccgggcc gatgcgcgcg gccggcgctg
     2221 gaacgccgca ggactcgcgc tctcgacgct gtacctcggc tgggggctgg gcgcgcagca
     2281 gctcgcgctc gcgcgcgccg aagccgccct cgccgccgcc ggccagccgg ccgtgcaggt
     2341 gctggcgctg ccgacggcgt tcaactcgct gctgtggcgc ctcgtcgtga tcacacccga
     2401 cgacggctgg ctggagggct acacggcgct gcccgatgcc ggcctgccgc gcttcgaacg
     2461 cttcccgcgc ggcgaggcgc tgttcgcgcg ctggcgccac gatccggccg tcgcccggct
     2521 cgcgcgcttc acgcacggtt actggcagat gcgcaggctg gcggacgggc gcgtcgtcat
     2581 caccgacctg cgcatgggcc agcagccgtg gttcacgttc gaattcgtcg tcgggcgcga
     2641 cctcggcgca catcgggagc ctgcgccggc gacgcgcatc cgcgaaccgc cgccggtcgc
     2701 gaccgtgctg cgctggatcg tgcaccgcat cggcgacgaa agcctgccgc cgccgcgcct
     2761 gccctgaacc gcgcttcagg gcaggcgcag cgcccctgcc gcctgcagcg ccccggcatc
     2821 gaaggtccgc gcccgtgcga aaccgccgac cagccgcgca cgctgcgggc gcagccggaa
     2881 cagccggaag tcgccgaagc cgaacagctg cgccgactcc ggcagccgcg ccagataacg
     2941 ggcctgcgcg gcgaggtagt cctcggtttc gcgcgccagc ggctcgacca tgccggacag
     3001 cgtcacgcgc gccagcgtct gcggatcggc gcggccgtcg tcgggctcgc cgatcgcgag
     3061 gctcgcgcgc gggtcagcaa gcagctgctt cgtgtgtgcg gcgagctgcg acaggtgcag
     3121 cagcacgccg tcgaacgcgc cttcgatcgc gtaggcgacg tgcgaggcct cgggaccgtc
     3181 gggcccgagc gtggcgagcg cggcccagcg ctggcggcgg atcagggtgg cgagttgcag
     3241 ggcgagatcg gggtccatga cttccggcgt gcgcgtggag gaatgaggaa agtccggggg
     3301 ctcaggccga cagccagacc agcgtcgcct gccggccgct gaccggtgcg cggcggtagg
     3361 aatgaaaggt gtcggcctcc gagtacgtgc agcgcccgcc accggacacc tgcccgacgc
     3421 cggcgcgcat gaggcgccgc cgcgcgagcg cgtacaggtc ggcgagcagg cggtcggcgc
     3481 gcgcgggcac gaaggcatcc gccgcgcccg ggtcgtcggc gacgaaggcc gcgcgcacct
     3541 ccgggccgac ctcgaacgcc gccggtccga tcgccggacc gagccaggcg agcacctcgg
     3601 ccggatcgac cgccatgcgc gcgagcgtcg cctccagcac gccgccggcg agcccgcgcc
     3661 agccggcgtg cgcggcggcg accacgcgcc cgccgcggtc gcagaacagc accggcaggc
     3721 agtcggccgt catcaccacg caggccacgc cgggcgtgcg cgtgatcgcg gcgtcggcgc
     3781 gtaccggcgc atgcacggtt tcggccgcgg cgaccgcgca gccgtgcacc tgttcgagcc
     3841 aggccggcgc gcgcgccagt ccgagcgccg cgctcagcag gcgccggttc tcggcgaccg
     3901 cggccggatc gtcgccgacg tgatcaccga ggttgagggc ggcgtacggg ccgcggctga
     3961 ccccgccgcg gcgcgtcgtc gccgcggcgc gcacgttcgc cggcgccggc cagtccggct
     4021 gcagcagggc aagcggttcc ccgctcattc gacgtactcc cattcgacgc catcggcatc
     4081 gacgccggcg cgtgcatcga gcccgtcgag gccggcgtcg cggcgcagca cgtcgagcag
     4141 cgcggcgaga tcgggcggcg tcggtgcgct ccagctcagg cgccggccgc tgcccggatg
     4201 cgtcagcgtc agccggcgcg cgtgcagggc ctggcgccgg aagcccttca gcgccgtcgc
     4261 gagttcgtcg ctgcagcccg gcggcaggcg cagccggccg ccgtagaccg gatcgccgag
     4321 cagcgggtgg tgcatgaagg ccatgtgcac gcggatctgg tgcgtgcggc cggtttcgag
     4381 cttcagcgcc agatgcgtgt gggcgcggaa gcgctcgcgc acgcggaaat gcgtgatcgc
     4441 cggcttgccg ccgggcgtgg cggccatgcg cttgcggtcg gtgccgtggc gggcgatcgg
     4501 cacgtcgacg agcccgcccg agaccagcac gccacaggcg atcgcctggt actcgcggcc
     4561 catcgtgcgc gcctgcagct gctcgaccag cgcggtatgc gcgcgcaggc tcttggcgac
     4621 gacgagcagc cccgaggtgt ccttgtcgag ccggtgcacg atgccggcgc gcggcagctg
     4681 cgcggtgacc ggcgcgtggt gcagcagcgc attgaccagc gtgccgtccg ggttgccggc
     4741 ggccggatgc acgaccagcc cggcgggctt gtcgatgacc aggatgtcct cgtcctcgta
     4801 gcgcacggcc agcgggatgt cctgcgggcg gtcggcgatg acttcggtgc gctcgtacgc
     4861 gccgctgacg cgctcgccgc cgagcaccag cgcccgcggc ttcgcgggcc tgccgtcgac
     4921 ggtcagcgtg ccgtccttca gccactgctg caggcgggcg cgggagtgat ccggcagcag
     4981 cgcggcgagc gcctggtcga ggcgctggcc ggcacactcc ggcgggatca ggcagtcgat
     5041 gcgttcaaat tcgggcatgg aacggctcgg acagggggcg agaggcggcc cggggtgcag
     5101 gcatggtctt cgttcggggg cgcaagatat actcggccgc cccgtcacct cgtacagatc
     5161 agccgaatgt ccgctcgctt cctgcgtctt cgcctcgcga ccgtcctggc cgctgccctg
     5221 cttgcgcccg gctgttcgtg gctgtcggag aaagccgacg agacccgcga ctggtcggcg
     5281 gcgaaactgt acagcgaggc caaggacgcg ctgaacaacg gcgattacac caaggccatc
     5341 ggctactacg acaagctgca ggcacgctac ccgttcgggc gctacgcgca gcaggcgcag
     5401 ctcgaatcgg tctacgcata ctaccgggac ggcgaacccg attcagcggt ggccgcggcc
     5461 gatcgcttca tcaagaccta tccgcgccac ccctacgtcg attacgtcta ctacctgaag
     5521 ggcctgacca actacaaccg cgccagcggt ccgctcgacc gcctgatgcc gccggacccg
     5581 acgcgcaccg atgcctcggc ggcgctgcag tccttcaccg acttcaacga cctcgtcacc
     5641 aaattcccgg acagccgcta cgcgccggat gcgcgcgcgc gcatgctgtt ccttcgcaac
     5701 aacctcgcca gctacgaact caacgtcgcg cgccactacc agagccgcgg cgcacacgtc
     5761 gccgccgtca accgcgccaa gtacgtggtc gagaactacg cgagcagccc ggcggcaacc
     5821 gaggcgctgg tgatcatggt gcgctcgtac acggccatgg gcctgcccga gctggcgcgt
     5881 gatgcgctgc gcgtgctgca gaagaacgat ccgcagaatc cggacatccc gaagctgacg
     5941 acgctggtcg gcacggcgcc gggcaagggc tgacagagag gcgcgccgcg catgtgggat
     6001 ttcttcgaga ccgtccgtca ccgtcactcg gtgcgcatct accagcagga catgccggtc
     6061 gacgaggccc gcctgcacgc gatcctggag atggcctgcg ccgctccgtc ggccggtgac
     6121 ctgcaggcct accgcatcgt cgtcgtgcgc gacgccgatg agcgccgggc gctgtcggtc
     6181 gcggccgaca atcagggctt catcgccgag gcaccggtat gtctggtgtt ctgcgccgat
     6241 ccggtgcgct cgggtgccga gttcggcgaa cgcggcgaac gcctgtacgc gctgcaggac
     6301 acgacgatcg ccgccgccta cgcgcagctt gcgatcgtcg ccgccggcat gggctcggcg
     6361 tggatcggct acttcgacga tcaggccgtg cgcgacctgc tcggcctcga cccggcgctg
     6421 cagccggtgg cgctgatgac ggtcggctac ccggccgaac tgccggtgcc gacgccgcgc
     6481 cggcggctcg cggaagtcgt cagcttccgc tgaggccgcc ggcggcggcg gcaacgcctc
     6541 agggctgcgc gccgaggaag ctcgcgagcg cctgcacgtc ggcctcggac agaccctgca
     6601 ccatcgagcg catcaccgaa tccggcgagc ggtcgtggcc ctggcggaag tcgttcagca
     6661 cgcgcagcag atagcccggc ttctggcccg cgagccgcgc ccggccgtcc ttgccgtgcg
     6721 catcggcgcc gtggcagctc tgacagcgcg ccgcgtacag cttcgccccg ctggcgacca
     6781 gcgcggcatc ggcggccggc tccggctgcg gcggctgcag cgcgtaatag acggcgagca
     6841 gcgccttctc gtcgtccgtg agcttcgcca tcaggtcgtt catgaccttg cgatagcgct
     6901 cggccccctg gaactggccg aactgcgtga gcagatactc gatcgactgc ccggcaaggt
     6961 ccggataatc cgctcgcgtc gattccccgc cggcgccgtg gcaggccgcg cagaaggccg
     7021 agcgctcgcg cccgcgctcc agcagatcgg cctgcacgcc cggattcgcc cgggcttcgg
     7081 caagcagcgc ctgcgcgcgc gtgagcagcg gagcgctgct gtcggcgtgg gccgccgccc
     7141 ccagcagcag ccacagcgcg gcgacggcat accgcatgcc ccgccccccg gctcgtctcc
     7201 tgaatcccgc gctcatcccc atcgactgcc ctcctcctta tggttgtgga caggctgctg
     7261 ctgattgctg attgttgatc gctgcctgcg gccccgtcag cgccggaacc cggacgtgat
     7321 cggatagcgc cggtcacggc cgaaggcgcg cggcgtgatg cgcacgcccg gggccgcctg
     7381 ccggcgcttg tattcgttga ggatcaccag ccgcgcgacg cgctcgacgg tggcgcgctc
     7441 gaagccgtcg gcgacgagtt cctcgaccga gcggtcgcgc tcgacgtagg cctccaggat
     7501 cgcatcgagc acgtcgtacg gcggcagcga gtcctggtcg gtctggtcgg ggcgcagctc
     7561 cgcggacggc ggccggtcga tgacccgctg cgggatgacg ggcgcctgcg cctggcggtt
     7621 gcgccacgca gccagccggt agaccagcgt cttcggtacg tccttgatcg gcgcgaaacc
     7681 gccggccatg tcgccgtaca gcgtcgagta gccgacggcg gtctcgctct tgttgctcgt
     7741 ggtcagcacc agccggccgg tcttgttcga cagcgccatc agcagcacgc cgcggcagcg
     7801 tgcctggatg ttttcctcgg tggtgtccgg cggcaggccc gcgaacagcg gttcgagcgt
     7861 gtgcaggaaa gcatcgaagg ccggctcgat cggcagcagg tggtaatcga cgccgagcgt
     7921 gcgtgcctcg atcgcggcgt cctcgttgga catgccggcg gtgtagcgcg agggcagcga
     7981 cacggcactg accgcatcgg ccccgagcgc atcgacggcg atcgccagcg tcagcgccga
     8041 atcgatgccg ccggagagcc cgagcaccgc gccgcggaag ccgttcttgc gcacgtagtc
     8101 gcgcgtgccg agcaccagcg ccgcgtagac ctcggcctcg tcctcggggg cttcggcgag
     8161 tggtccgggg cgcggcacca gcgcgccgtc gatgcgatcg aaaccaagcg gatacaggcc
     8221 ctcgacgaag gccggcgcgc gcgcgagcag cgtgccgtcg gcatcgacga gcagcgagcc
     8281 gccatcgaac accagttcgt cctgaccgcc gacctggttg acgtagacga tcggcacacc
     8341 ggcctcgtgc acgcgcgcgt gcaggatcgt cagccgctcg ccggccttgg cgcggtgcca
     8401 cggcgaggca ttgaggttca ggatcagctc ggccccggcc tgcgcggcct gcgcgaccgg
     8461 accgggctgc cagatgtcct cgcagaccgt cagcccgaag cgggtgccga ggtgctcgac
     8521 cacgcacggc ccgctgccgg gcacgaagta gcgcttctcg tcgaacacgc cgtagttcgg
     8581 cagcagctgc ttggcgtaga cgccgagcgc ccggccgccg gcgaggaagg acgcagcgtt
     8641 gtacaggcca ccgagaccgc gctgcggatg accgatcacc agcgccggcc cgtgcgcggc
     8701 cgcggtcgcg agttcggcga gggcggcctc gcagcggctg acgaattcgg ggcgcagcag
     8761 caggtcctcg ggcggatagc cgcacagcgc cagctccggg aacaccacga cgccggcgcc
     8821 gagttccgtc tgcgcctgcc gggcgagttc gaggatgcgg cgcgtgttgc cggcgatgtc
     8881 gccgaccagc gggttgaact gcgccatcgc gacggtcagg gcagcggggg cggcggcact
     8941 catcgacgga ctccggaagt cgggacggac gggggacgaa cgggacggac ggagcctgtc
     9001 atcagaacac aaacccgcag cccgcaaaaa gcgaagggcg aggtccgcga ccccgccctt
     9061 cggtcagacg acccgccgga cgctgccggc gggcggtgac ggctcagccg gcgaagaagc
     9121 cgttgatcgc ctcgccgatg tcggccggcg aacggacggt cttgacgccc gcggcctcga
     9181 gggcggcgaa cttgtccttc gcggtgccct tgccgccggc gatgatcgca ccggcatggc
     9241 ccatgcgctt gcccgggggc gccgtgacgc cggcgatgta ggcgacgacc ggcttgcgga
     9301 cgctggcctt gatgaactcg gccgcttcct cctcggccga accgccgatc tcgccgacca
     9361 tgacgatgcc cttggtggcg ctgtcggcct cgaacatctc cagcacgtcg atgaagctca
     9421 tgccgtggat cgggtcaccg ccgatgccga cgcaggtcga ctggccgaga ccgatgcgcg
     9481 tggtctggtg cacggcctcg taggtcagcg tgcccgagcg cgagacgatg ccgatcttgc
     9541 ccggctggtg gatgtggccg ggcatgatgc ccatcttgca ctcgcccggg gtgatcaggc
     9601 cggggcagtt cgggccgatc agccggacgt ccgagccctt cagcgcggcc ttgaccttca
     9661 gcatgtcgag gaccgggatg ccctcggtga tgcaggcgat gaccttgatg ccggcgtcgg
     9721 cggcttcgag gatcgcatcg gccgcgaacg gcgccggcac gtagatcatc gaggcgttgg
     9781 caccggcgtc gcgcacggct tcatggacgg tgttgaacac cggcttgccg aggtgggccg
     9841 aaccgccctt gcccgggctg acaccaccga ccaggttggt gccgtaggcc aggcactgct
     9901 cggagtggaa cgtaccctgc gaacccgtga agccctggca gatcagcttg gtgttcttgt
     9961 cgatcaggat gctcatgtaa ctccccctct cacttcgcgg cggcgacggc ggccttggcg
    10021 gcttcggtca ggtcgtcggc agcgatgatg ttcatgccgc tttccgacag catcttgcgg
    10081 ccggcctcga cgttcgtgcc ctgcaggcgc acgacgacgg gaatggtcag gccgacttcc
    10141 ttgaccgcgg cgatgatgcc ttccgcgatc aggtcgcagc ggacgatgcc gccgaagatg
    10201 ttgacgaaga tcgcgcggac cttgtccgag gagaggatga gcttgaacgc cttggcgacg
    10261 cgctcggccg tggcaccgcc gccgacgtcg aggaagttcg ccggctcgcc gccgtgcagc
    10321 ttgaccatgt ccatggtcgc catcgccaga ccggcgccgt tgaccatgca gccgatgttg
    10381 ccgtcgagcg cgacgtagtt caggccgatc tcgtgcgcga ggttctcgcg ctcgtcttcc
    10441 tgcgtcgaat cgcgccactc ggcgatgtcc ttgtggcggt acagcgcgtt gtcgtcgagg
    10501 ccgatcttgg catcgagcgc gagcagcttg ccctcgccgg tgacgatcag cgggttgatc
    10561 tcgaccagcg aggcgtcctt ggcgacgaac atgtcataga gaccgttcat gaccttgacc
    10621 agatgatcga cctgcggctt ggcgagtccc atgccgaagc ccagctggcg ggcctcgtag
    10681 gcctggaagc cggcggccgg atcgatggcg acggtcagga tcttctcggg cgtgctttcg
    10741 gcgacttcct cgatgtccat gccgccttcg gaagaaccga tgaacgcgat gcgcttggtg
    10801 ctgcgatcga ccagcaccga caggtacagc tcatcgacga tgttcaggcc ttcctcgatc
    10861 agcagtgcat ggatcggcag gccctcggga ccggtctgct tggtcgcgag ccgggtgccg
    10921 agcagcgagc cggcgatgtc ctcgacctcc tgcaggctgc gcgccagctt gacgccaccg
    10981 gccttgccgc ggccacccgc gtgcacctgt gccttgacca cccacacgga gccccccagc
    11041 tccttggcgg cggccacggc ttccttcggc gaagttgcct tgatgccgcg cggcaccgcg
    11101 acgccgaact ggcggaaaag ctcctttgct tggtattcgt gcagattcat gcgaattcca
    11161 ccccctttcg ataggagccg gtatcctatc ggacggcgcc acaaaagcct atcggccgcc
    11221 gcccgtccgc aagcgtgttg cgcgcaccgc ccatgagtcg atacctgctg ctcctgctgc
    11281 tcgtcctcgc cgccgtctgg tggctgcgcc gcgcccggcg cccgcaagcc ccggcaaggc
    11341 cgcaggcacc ggcacaaccg gcaaaacctg actggcctgc aatggtgcgc tgcacggagt
    11401 gcggcataca cttcccggca accgatgcgg tgcgcgatgc cgatgcgcgt ccgtattgct
    11461 gcccggcgca ccgcgagcgc catgcgctgc gcccacccaa ggactgaacg gccgccgccg
    11521 cacgggcgcc ggccccgcct gaggccctgc tgaacgatga tcgttacgga cagcgtcatg
    11581 gaccggcgac gcggctggtc ggtgctgcgc gcctacgtgc tgtaccgcct ggtgctggcc
    11641 acgctgctgc tcggcatcac gctcgccgat ctggccggca aactgttcgg tgccgaagac
    11701 ccgcgcctgt actggtggac gatcggcggc tatttcggcc tgctcgccac cggcttcctg
    11761 ctgagctacc tgcgccggcc cgacctcatc gtgcaggcga cgctgcacgc cggcctcgac
    11821 atcgtcgtgt tcacggcgct gatgtacgca agcggcgggg cttccggcag tctcggggcg
    11881 ctgctggtgt cggcgatcgc cggcagcgcg atcctgctgc cgcgccggct ggtgatcacg
    11941 gtcacggccg cggcggccct cgcgatcatc ggcgtatggc tgtacggcat ctggcaaagc
    12001 ttcgcgctgc gcaccggcgc gccgtacctg agcctcgacc tgctgccgcc gctggtggac
    12061 tgggcgctcg tgcatggcgc ctccggcggc cagacggcgc tgatctgcct ggcgctggcc
    12121 gccgccgccg ccgccgcgta cacgatcgcc gagcgcacgc gcaacagcga gaccctggtg
    12181 ctcgaacgca cgcaggagtt gctggagatg gcggagctga acgcggcgat catccgccac
    12241 ctgcagtccg gcatcctcgt cgtcgaccgt cacgcgcgcg tgaagctgat gaaccagatg
    12301 gcggccgagc tgctcgactt ccacgagcgc ccgatcggcc tgccgctgtc ggacatctcg
    12361 ccgctgctgt cgcagcgcct cggcgcctgg atcggctcgg gcctgacgca gacccagccg
    12421 ttccggcccg ccgagcacct gccggagctg atcccggagt tcacggcgct gtccggcaac
    12481 ctcgccttcg agacgctggt cttcatcgag gactcgaacg ccgccgccgc acggctgcag
    12541 cagatcaagc tcgccgcgct cgggcgcctg accgcgagca tcgcccacga gatccgcaat
    12601 cccctcgcct cgatcagcca cgccgcgcag ctgctggtcg aatcgaccgc caaccccggc
    12661 gagaaacgcc tcggcaagat catccacgac aacgccgggc gcgcgaacca gatcatcgcc
    12721 aacgtcctcg acctgtcgcg gcgcgacaag gccagtcccg aggacatcca cctgcgcagc
    12781 tggctggagg acttctgcca cgacttcatg cgcgggcgcg gcgatccgat gccggagatg
    12841 gaaatccgcg tgcagccgga agacctcacc gtgcatttcg atgccggcca cctgcagcag
    12901 gtgctgtgga acctctgcaa caacgcctgc gagcacggca cgctgcccgg cgaatcgccg
    12961 cgcgtgcgca tcctcgccgc gctcgacccc gagcgcttcc ggccctacct cgacgtcatc
    13021 gacttcggcg ccggcatccc cgaggccgag gcgcgccaga tcttcgagcc cttcttcacc
    13081 accaatccga agggcaccgg tctcggcctg tacctgtcgc gcgagatgtg cgaggccaac
    13141 cgcgcacagc ttcagtaccg cccgaacccc tccggccagg gcagctgctt ccggctgacc
    13201 ctgccgaccg ccacccgcac gccggcgacg acgatgaaca cgacggagga accgacgtgg
    13261 aatcttgccg ggcgctgatc ctcgacgacg aacccgacct gcgcgacctg atcgccctga
    13321 ccctcgaacc gatgggcgtg cagtgcgtgc cggccgcgac gctctcggcc gcccgcaccg
    13381 cgctggccag ccaggggccg ttccatttct gcctgaccga catgcgcctg cccgacggca
    13441 acggcctcga tctggtggcg ctgatccagc gccagtaccc cgagatgccg gtggcggtga
    13501 tcaccgcgca cggcaacgtc gagagcgcgg tcgaagccct gaagctcggt gccttcgact
    13561 tcgtctccaa acccttcgac atcgccacgc tgcgggcgat ggtcgatcag gcccggcggc
    13621 tggccggcgt gccggccggg cgcgacggcg gcccgaaccg gcccgacctg atcggcgcca
    13681 gcccggcgat ggagcgcgtg cgctcgatga tccgcaagct cgcgcgcagc caggcgccgg
    13741 tgttcatcgg cggcgaatcc ggcaccggca aggaggtcgt cgcgcgcctg atccacgctt
    13801 ccggctcgcg cgccgagcgg ccgttcgtgc cggtcaactg cggtgcgatc ccggcagaac
    13861 tgatggagag cgaattcttc ggccaccgca agggtgcctt caccggcgcg gttgccgaca
    13921 agcccggcct gttccaggcc gcacacggcg gcacgctgtt cctcgacgag gtcgccgaac
    13981 tgccgcaggc catgcaggtc aagctgctgc gcgcgatcca ggagcgcgcg atccgcccga
    14041 tcggcggcgc gcacgaactg ccggtcgacg tgcgcgtgct gtcggccagc catcgcgacc
    14101 tcgccgccga ggtgcaggcc gggcgcttcc ggcaggattt ctactaccgg ctcaacgtca
    14161 tcgaa
//
LOCUS       DAOQIE010000055        14156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2061_length_14156_cov_10.381462, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000055 DAOQIE010000000
VERSION     DAOQIE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2061_length_14156_cov_10.381462"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1615
                     /gene="ahpF"
                     /locus_tag="PK144_05300"
     CDS             35..1615
                     /gene="ahpF"
                     /locus_tag="PK144_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNF65175.1"
                     /translation="MLDNTLKTQLKAYLERIVQPVTLSASLDDGDKSREMRGLLEDIA
                     ALSGGRVTLETASAAAAGVHVPSFTVGRTAAAGSAGIRFAGIPLGHEFTSLVLALLQT
                     GGHPPKVAPELIEQIRALPGEYRFQTYISLSCQNCPDVVQALNLMAVLNPRVTHEMID
                     GALFQDEVERLSIMAVPTVLLNGETFGQGRMSLEEIVAKLDSGAGARAADRLNAKEPF
                     DVLVVGGGPAGAAAAIYAARKGIRTGVAAERFGGQVLDTLGIENFISVKETEGPKLAA
                     ALEEHVRSYEVDIMNLQRATKLVPGTDGAPAEVELASGARLKAKTVILATGARWRDMN
                     VPGEQEYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFAP
                     ELKADAVLQRKLHSLPNVTVIVQAQTTEVTGDGQKVNGLRYTDRASGEAKRIELEGIF
                     VQIGLLPNTDWLRGTVELSRFGEIIVDAKGQTSVPGVYGAGDCTTVPYKQIVIAMGQG
                     STAALAAFDHLIRTSAPA"
     gene            complement(1760..2233)
                     /locus_tag="PK144_05305"
     CDS             complement(1760..2233)
                     /locus_tag="PK144_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009505793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF65176.1"
                     /translation="MPHAALDRYDLKILGVLQREGRIPNNELADRVGLTPSPCLRRVR
                     ALEEAGVIRRYVGLVEPGAVGLPISVFVHISLTSQERASLETFETAVRAFPEVMECYL
                     MTGVADYLVRVVVPDLDAYERFLLDRLTRVPGVTNIRSSFALKQVIYKTELPLGD"
     gene            complement(2320..2721)
                     /locus_tag="PK144_05310"
     CDS             complement(2320..2721)
                     /locus_tag="PK144_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF65177.1"
                     /translation="MRYLLDTNICIYISKCRPLLVRQRFENIESAAVGMSVITWGELC
                     FGARKSQSSQRAMEVLEQLQSMIRVVPITTEVGEHYGDIRASLERGGRPIGNNDLWIA
                     AHARALACTLVTNNEREFVRVPDLSVENWLN"
     gene            complement(2727..2969)
                     /locus_tag="PK144_05315"
     CDS             complement(2727..2969)
                     /locus_tag="PK144_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNF65178.1"
                     /translation="MAIARIFKSGNSQALRLPKEFRFAGDRVEILRRGEEIVLRELPV
                     SAAAIFDALAALPDDLFAEGRPDGPDDMPQERKPLL"
     gene            3189..5918
                     /gene="aceE"
                     /locus_tag="PK144_05320"
     CDS             3189..5918
                     /gene="aceE"
                     /locus_tag="PK144_05320"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNF65179.1"
                     /translation="MHASAQPQPSAAAAEIPGSASDRAALEADRRDWIDSLAGLHAAH
                     GPAGVREILQSLQQYALAHELAPVEAVLNTPYRNTIAVSAQAPYPGDAELEARIANIN
                     RWNAMAMVLQAADSGSGVGGHIATYLSAATLLEVGFNHAFRARSADYGGDLLICQPHA
                     APGVYARAFLEGRLSAVQLGNFRRELGAGGGLPSYPHPRRLPDFWQAPCASMGLSTPT
                     AIHQARFRKYLENRGLAPAGGGGRVWCFIGDGEADEPEVLGTIGIAARERLDNLVLVV
                     NCNLQRLDGPVRGNGKIIQELERSFRGAGWNVLKVIWGSGWDPLFARDRDGSLIRRME
                     SAVDGDYQRYTVLPGEAVREHWVLDNPALAELMASLSDEEIRSIKRGGQDPLKIWAAY
                     AQAMQPNGRPTVILAKTVKGDGMGPGGEGRNTAHQKKQLSADERRDCARRWGIPLADE
                     DIVRAAFYRPAADSPELRYLHARRERLGGYLPARRVDCPPLPAPAAATLAESFEGSGE
                     RALSTTTAAVRLLGRLLRDPALGRLIVPIVPDEARTFGMEGLFRSAGIYSPEGQRYVP
                     VDGDTLLPYREARDGQILQEGICEAGAMASFLAAGTAYAEHGVPSIPFYFFYSMFGSQ
                     RVGDLVWACGDMLARGFLLGGTAGRTTLNGEGLQHQDGHSPLLAATVPNLKVYDPAWG
                     WELATIVEDGIERMYGRGEAVFYYLTVYNEAYPMPARPADPAAREGVLRGLYRLREPA
                     LPGAGPVRVNLFGSGSLLPQALAAAELLEARGFATAVWSATSYVELQREALACARWNR
                     LHPDETPRVSHIERVLAGVPGVFVAVSDYQKSLALGIAPWVPGRYEVLGTDGYGLSEA
                     RERLREHFGVSPAHIAWAALHALAAGGGLDATRLATARHELGIDAGQPDPAAF"
     gene            complement(5965..6318)
                     /locus_tag="PK144_05325"
     CDS             complement(5965..6318)
                     /locus_tag="PK144_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65180.1"
                     /translation="MIRTSFRNEFRNNLRKGLRTVLIACPLSAGLAFASGAAFAAGQP
                     AHGHGSPVQLADAHGSRPLPVYGRAPMTADACHGASPRGCRDDAEAGRREAVQPLLCL
                     GASPRGCLLRPASEV"
     gene            complement(6502..7584)
                     /locus_tag="PK144_05330"
     CDS             complement(6502..7584)
                     /locus_tag="PK144_05330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNF65181.1"
                     /translation="MSASALLTRCLPGLALAAGLALGSATAAAAPYTDFYVFGDSLSD
                     TGNVALVSGGTVPPAPYFNGRFSNGPNWADRFAAEGSLFTSTQTPLLPGSVLGNVGDN
                     YAWGGARTYGHSNPALNSIARLSSQIGAFASQHAGVADPDALYVIWIGANDMQDAVVA
                     AATAYQNALIGGATPAAAQLIALPLINSAVTVAISNINSAISSLLALGADEFLVANLP
                     DLSLTPRLAPAGLFAQYASTSFNAALLALLQTRPGTAHLADIQSAYNELLGNPAAYGF
                     TNTTAPCYTGDDITFTPNTGTVCANPDQYVFWDTFHPTAAASAWIEAEMVSALAATIP
                     EPASYALLGAGLLAFGRARRQAGRRD"
     gene            7996..9228
                     /locus_tag="PK144_05335"
     CDS             7996..9228
                     /locus_tag="PK144_05335"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNF65182.1"
                     /translation="MSTPPLIARQPVYDARHHVLAWDIAHARAQHPAATPPAEPDSEA
                     AEAAHFELLTGALLVAGLERLIGNGFGFLPVSAHTLLSPTLRLLPPLQVVLTLPAAGL
                     QSVDLPARLAPLQRAGYRVALDGCRSLEPGLLPLLPDLDFLRLDAAALGAAALAPLLQ
                     SLRGAGFHGVAIATGIADAGTAQAALDAGVLGLQGPLFRRPQPVAASALDTGREAALT
                     LIAALNRPDAGLPEISNLIRRDATLTYKLLRYVGSAWFGLGEVHSVDHALICLGRLRL
                     RLWATLVAMTGAAGERHDLLELALVRAGMCRDLLLAEGGDAETGTLAGLLSTLDLLFG
                     LPLERVLAGLPLNRELRQALLEHAGPAGRALALALAYERGDWEEVHAAGRDEQVFDAY
                     FAALAQAHETLEQIGTSH"
     gene            9353..10258
                     /locus_tag="PK144_05340"
     CDS             9353..10258
                     /locus_tag="PK144_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNF65183.1"
                     /translation="MSLPESVKIVEVGPRDGLQNEAAAVPTEIKVELIERLTAAGLPA
                     IEATSFVSPKWVPQMADSAEVLARLRRPPGCSYPVLTPNLKGFEAALAAGAREVAVFA
                     AASETFSQRNINCSIAESLARFEPLLAAAEAAGVRVRGYVSCVAGCPYEGAVAPSAVA
                     RVALELLRMGCYEISLGDTIGIGTPATMLAMLDAVGGAVSPARLAVHCHDTRGMGIAN
                     VYACVQAGITVVDASVAGLGGCPYAPGASGNVATEDVVWLLDGLGVHTGVDLVKLAET
                     GQWISDRLGRPNGSRAGRALLALRH"
     gene            10405..12360
                     /locus_tag="PK144_05345"
     CDS             10405..12360
                     /locus_tag="PK144_05345"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNF65184.1"
                     /translation="MTDIPLWQPAPKRIARANLTAFRTAVNAAHGLDLPDYPALYDWS
                     IREPEAFWRTLWDWADVIGERGERTLTGAGSMPGAQWFPDAQLNFAENLLRRNDDSTA
                     IVFRGEDKVEKRLSWRELNAAVSRFAQALLNERVQPGDRVAAFLPNLPETVIGMLAAT
                     SIGAVWSSCSPDFGTQGVLDRFGQIEPVVLIGCDGYWYNGKRIDTLHKLGEIRAQLPS
                     VRRLVVVGYLDAIPEVSAIPDAVRLDEFIAHWPAHTPEFRRQPFSHPLYIMFSSGTTG
                     VPKCIVHGAGGTLLQHLKEHRLHTDVRPGERVFYYTTCGWMMWNWLVSALASEATLLL
                     YDGSPFAPDGNVLFDYAQAERMNVFGTSAKYIDACAKAGIAPIDTHALPELRAILSTG
                     SPLVPESFDYVYARVKSDVQLSSISGGTDIVSCFALGNPVLPVWRGELQCRGLGLKVE
                     VWNDDGQPVRGEKGELVCTAPFPCMPIGFWNDPEGVKYRAAYFERFPGIWCHGDYVEL
                     TAHDGLIIYGRSDAVLNPGGVRIGTAEIYRQVEQLDEVVESLVIGQDWHGDVRVVLFV
                     HLRPGVALDDALQKKIRDHIRARTTPRHVPAKIVQVEDIPRTKSGKIVELAVRDIVHG
                     RPVKNQEALANPGVLAQYANCEELTRD"
     gene            complement(12395..12934)
                     /locus_tag="PK144_05350"
     CDS             complement(12395..12934)
                     /locus_tag="PK144_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65185.1"
                     /translation="MAAIIFLSRTEYSAMLRRFTLQRSLAPALAGILMISALPAPAQQ
                     QGNSNAASASSTPTSCEDAVAQQVRKQCPREASRVCAEAVRKAVAQSCSPQQQVRNQQ
                     LAQQTSQVPSPETAGTGAGETGLAAGAVEATVLGVPVIPLGIATVLAVGIGVAAGGGS
                     NGGGEDSSGSSSAATPSHH"
     gene            complement(13128..>14156)
                     /locus_tag="PK144_05355"
     CDS             complement(13128..>14156)
                     /locus_tag="PK144_05355"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNF65186.1"
                     /translation="LNTNDAPVAANDAVTTDMETAIVINVLANDSDVDADDALRVTAV
                     ESPAHGTVRIEADGRIYYEPEFGFSGQDAFRYTVTDRNGATAIGTVQVTVLETRSPVP
                     VNSTAPLSPTSAPAPTTFAPTSAVQPNLVLAAVTGDSGIGSDDAVRDAVYGNDTLMVR
                     PPAPGVGFEQVRNTLQTYSNPIPPLSTPNAPPPLLIPGLRPDNGTGTGFERPALAPES
                     LRAGSSFGFSLPPLTAGGTREVRLSTGEALPAWMNFDPATGRISGQVPDGVRGTIELR
                     IRERDAGGAVTERVLQIEIDAPANDEAPPSADAVPLPAGLSAQLQQIGNRFDAGRATL
                     LARLAALG"
BASE COUNT         2144 a         5001 c         4899 g         2112 t
ORIGIN      
        1 ccgcccgccc gcccgatcga cggaagccgc agccatgctc gacaacaccc tcaagacgca
       61 gttgaaagcc tacctcgaac gcatcgtgca gccggtgacg ctgagcgcca gcctcgatga
      121 cggcgacaaa tcccgcgaga tgcgcggtct gctcgaagac atcgccgcgc tgtccggcgg
      181 ccgcgtgacg ctcgaaaccg cgagcgcggc agccgccggc gtgcacgtgc cgtcgttcac
      241 ggtcgggcgc accgccgccg ccggcagcgc cggcatccgt ttcgccggca tcccgctcgg
      301 ccacgaattc acgtcgctgg tgctcgcgct gctgcagacc ggcggccacc cgccgaaggt
      361 cgcgcccgag ctgatcgagc agatccgcgc gctgcccggc gaataccgct tccagaccta
      421 catctcgctg tcctgccaga actgcccgga tgtcgtgcag gcgctgaacc tgatggcggt
      481 gctgaacccg cgcgtcacgc acgagatgat cgacggtgcg ctgttccagg acgaggtcga
      541 acgcctctcg atcatggccg tgccgaccgt gctgctgaac ggcgagacct tcggccaggg
      601 ccgcatgagc ctcgaagaga tcgtcgcgaa gctcgacagc ggcgccggtg cccgtgcggc
      661 cgatcgcctg aacgccaagg agcccttcga cgtgctcgtc gtcggcggcg gcccggccgg
      721 ggcggcggcg gcgatctacg ccgcgcgcaa gggcatccgc accggggtcg cggccgagcg
      781 cttcggcggt caggtgctcg acacgctcgg catcgagaac ttcatctcgg tcaaggaaac
      841 cgaaggcccg aagctcgccg ccgcgctcga ggagcacgtg cgcagctacg aggtcgacat
      901 catgaacctg cagcgcgcga cgaagctcgt gcccggcact gacggcgccc cggccgaggt
      961 cgagcttgcg agcggggcgc ggctgaaggc gaagaccgtg atcctcgcca ccggcgcgcg
     1021 ctggcgcgac atgaacgtgc cgggcgagca ggaataccgc acgaagggcg tgacctactg
     1081 cccgcactgc gacggcccgc tgttcaaggg caagcgcgtg gcggtgatcg gcggcggcaa
     1141 ctccggcgtc gaggcggcga tcgatctcgc cggcatcgtc gcgcacgtga cgctgctcga
     1201 attcgcgccg gagctgaagg ccgatgccgt gctgcagcgc aagctccaca gcctgccgaa
     1261 cgtcacggtg atcgtgcagg cgcagaccac cgaggtcacc ggcgacggcc agaaggtcaa
     1321 cggcctccgg tacaccgacc gcgccagcgg cgaggcgaag cgcatcgagc tcgaaggcat
     1381 cttcgtgcag atcggcctgc tgccgaacac cgactggctg cgcggcacgg tggagctgtc
     1441 gcgcttcggc gagatcatcg tcgatgcgaa gggccagacc tcggtgcccg gcgtctacgg
     1501 tgccggcgac tgcacgacgg tgccgtacaa gcagatcgtc atcgccatgg gtcagggttc
     1561 gaccgccgcg ctcgcggcct tcgaccacct gatccgcacc tcggccccgg cctgaacctt
     1621 cccgcccccg ccgccgccgc gcggcgggga gcgatccggt ttccgccccc cgcggccggt
     1681 gcaggtgttc cacgtgaatc ctgtgccggt gcgggggttt ttttccgtcg cggcttcgtt
     1741 tgcgccgtcg tttgcgcgtt cagtcgccga gcggcagttc ggtcttgtag atgacctgct
     1801 tcagcgcaaa gctcgaacgg atgttggtca cgcccggcac gcgcgtcagc cggtcgagca
     1861 ggaagcgttc gtaggcatcg agatcgggca cgaccacgcg caccaggtaa tcggcgacgc
     1921 cggtcatcag atagcactcc atgacctccg gaaacgcgcg cacggcggtc tcgaaggttt
     1981 cgaggctggc gcgctcctgg ctggtcagcg agatgtgcac gaacacgctg atcggcagcc
     2041 cgaccgcccc cggctcgacc agcccgacgt agcggcggat caccccggcc tcctccagcg
     2101 cacgcacgcg gcgcaggcac ggcgacggcg tcaggccgac gcgatcggcc agctcgttgt
     2161 tcgggatgcg gccttcgcgc tgcagcacgc cgaggatctt caggtcgtag cgatcgagtg
     2221 cggcatgcgg catgttctgt ctccccggta tcgttatccg gcagagtgtt gcgcatcgac
     2281 gcagcaaaat ccgcatccgc ttgcaaaaag ccgtaccggt cagttcaacc agttttcgac
     2341 cgaaagatca ggaacacgca cgaactcgcg ctcattattg gtaacgagcg tgcatgccag
     2401 tgcccgcgca tgtgcggcaa tccatagatc gttgttaccg atcggccggc ccccacgctc
     2461 cagcgaggcg cggatgtcgc cgtagtgttc gccaacctct gtcgtgatcg gcacgacccg
     2521 gatcatggac tgaagctgtt cgagcacttc catggcacgt tgggatgact ggctcttgcg
     2581 agccccgaag caaagctccc cccaggtgat gaccgacatg cccactgcgg cagattcgat
     2641 gttctcgaat cgctgccgaa caagcagagg tctgcatttg ctgatgtaga tacagatgtt
     2701 ggtatcgagc agatagcgca gatcgttcac agcaggggtt tccgctcctg cggcatatcg
     2761 tccggaccat ccgggcgacc ttctgcaaac agatcgtcag gcagcgccgc gagcgcatca
     2821 aaaatggctg cggcactgac cggcaattca cgcagcacga tttcttcacc ccggcgaagg
     2881 atttcgaccc gatcgccggc aaacctgaac tccttgggca gacgcaaggc ctggctgttg
     2941 ccggatttga aaatgcgtgc gatggccatc gtggtcccct cgaaggatat acgcaaagta
     3001 tatacatcct gtgcaagcct ccttcacacg cccggaagca agaacaacaa atcccgcaat
     3061 ttcccatcga aacagcatct ttgttgcgca ggacaaggtc acatcaccca catacgcaat
     3121 cgcccgccct gcccgcctcc ctagactggc cgccatcccg ccaccattgc ccaggagagg
     3181 ccgccgccat gcatgcgtcc gcccagcccc agccgtccgc ggctgcagca gaaatccccg
     3241 gcagcgcgtc cgatcgcgcc gccctcgaag ccgatcgccg tgactggatc gattcgctcg
     3301 ccggcctgca cgccgcacac gggccggccg gcgtgcgcga gatcctgcag agcctgcagc
     3361 agtacgcgct cgcgcacgag ctggcgccgg tcgaggcggt gctgaacacg ccgtaccgca
     3421 acacgattgc cgtgagcgcg caggcgccgt atccgggcga tgccgagctc gaagcgcgca
     3481 tcgccaacat caaccgctgg aacgcgatgg cgatggtgct gcaggcggcc gacagcggca
     3541 gcggcgtcgg cggccacatc gccacctacc tgtcggcggc gacgctgctc gaagtcggct
     3601 tcaaccacgc cttccgcgcg cgcagcgccg actacggcgg tgacctgctg atctgccagc
     3661 cgcacgccgc gcccggcgtc tacgcgcgcg ccttcctcga aggccggctg agcgccgtgc
     3721 agctcggcaa cttccgccgc gaactcggcg cgggcggcgg tctgccctcg tatccgcatc
     3781 cgcgccggct gccggatttc tggcaggcgc cgtgcgcatc gatgggcctg tcgacgccga
     3841 ccgcgatcca tcaggcgcgc tttcgcaagt atctggaaaa ccgcggcctg gctccggccg
     3901 gcggcggcgg ccgggtctgg tgcttcatcg gcgacggcga ggccgacgag cccgaggtgc
     3961 tcggcacgat cggcatcgcc gcgcgcgagc ggctcgacaa tctggtgctg gtcgtcaact
     4021 gcaacctgca gcgcctcgac ggcccggtgc gcggcaacgg caagatcatc caggagctcg
     4081 aacgcagctt ccgcggcgcc ggctggaacg tgctgaaggt gatctggggc agcggctggg
     4141 atccgctgtt cgcgcgcgat cgcgacggca gcctgatccg gcgcatggag tcggccgtcg
     4201 acggcgacta ccagcgctac acggtgctgc ccggcgaggc ggtgcgcgag cactgggtgc
     4261 tcgacaaccc ggcgctcgcc gagctgatgg cctcgctgtc ggacgaggag atccgctcga
     4321 tcaagcgcgg cggtcaggac ccgctgaaga tctgggccgc ctacgcgcag gcgatgcagc
     4381 cgaacggccg cccgaccgtg atcctcgcca agaccgtcaa gggcgacggc atgggtccgg
     4441 gcggcgaggg ccgcaacacc gcgcaccaga agaagcagct gtcggccgac gagcggcgcg
     4501 actgcgcgcg gcgctggggc atcccgctcg ccgatgagga catcgtgcgc gccgccttct
     4561 accgtccggc cgccgacagc ccggagctgc gctacctgca cgcgcggcgc gagcgcctcg
     4621 gcggctacct gccggcgcgg cgcgtcgact gcccgccgct gccggcaccg gccgcggcga
     4681 cgctcgccga gagcttcgag ggaagcggcg aacgcgcgct gtcgacgacg accgccgccg
     4741 tgcgcctgct cgggcggctg ctgcgcgacc cggcgctcgg gcggctgatc gtgccgatcg
     4801 tgcccgacga ggcgcgcacc ttcggcatgg aagggctgtt ccgcagtgcc ggcatctact
     4861 cgcccgaggg ccagcgctac gtgccggtcg acggcgacac gctgctgccc taccgcgagg
     4921 cgcgcgacgg gcagatcctg caggaaggca tctgcgaggc cggcgcgatg gcctccttcc
     4981 tcgctgccgg caccgcctac gccgagcacg gcgtgccgag catcccgttc tacttcttct
     5041 attcgatgtt cggcagccag cgcgtcggcg atctggtctg ggcctgcggc gacatgctgg
     5101 cgcgcggctt cctgctcggc ggcacggccg ggcgcacgac gctcaacggc gagggcctgc
     5161 agcaccagga cggccactcg ccgctgctcg ccgcgaccgt gccgaacctg aaggtctacg
     5221 acccggcctg gggctgggag ctcgcgacca tcgtcgagga cggcatcgag cgcatgtacg
     5281 gacgcggcga ggcggtcttc tactacctga ccgtctacaa cgaggcctac ccgatgccgg
     5341 cacgcccggc cgatccggcg gcgcgtgaag gcgtgctgcg cggcctgtac cggctgcgcg
     5401 aaccggcgct gccgggggcc ggacccgtgc gcgtcaacct gttcggcagc ggcagcctgc
     5461 tgccgcaggc gctggcggcg gcggaactgc tcgaagcgcg cgggtttgcg accgcggtct
     5521 ggagcgcgac cagctacgtc gagctgcagc gcgaggcgct cgcctgcgcg cgctggaacc
     5581 gcctgcatcc ggacgaaacc ccgcgcgtca gccacatcga gcgcgtgctc gccggcgtgc
     5641 ccggcgtgtt cgtcgcggtc agcgactatc agaagtcgct cgccctcggc attgcgccgt
     5701 gggtgccggg gcgctacgag gtgctcggca ccgacggcta cgggctgtcg gaggcgcgcg
     5761 agcggctgcg cgagcacttc ggcgtcagcc ccgcgcacat cgcctgggcg gcgctgcacg
     5821 cgctggcggc cgggggcgga ctcgacgcga cgcggcttgc gacggcgcgg cacgaactcg
     5881 gcatcgacgc cggccagccc gatccggcgg ccttctgagc ccccggccac cgccggtcgc
     5941 ggcggcacca gcgcggcggc cggttcacac ctcgctcgcc gggcgcagca ggcagccgcg
     6001 cggcgaggcg ccgaggcaga gcagcggctg cacggcctcg cggcgaccgg cttcggcgtc
     6061 gtcgcggcag ccgcgcggcg aggcaccgtg gcaggcatcg gccgtcatcg gcgcgcggcc
     6121 gtacaccggc agcggccggc tgccgtgcgc atcggcgagc tgcaccggcg agccgtggcc
     6181 gtgggccggc tggccggcgg cgaacgcagc accgctggcg aacgcgagcc cggccgacag
     6241 cgggcaggcg atcaggacgg tgcgcaggcc cttgcggagg ttattgcgga attcattgcg
     6301 gaagcttgtg cggatcatgg cggcatctcc gtgaaacagg cgggtatcgt cgggttgcgg
     6361 aagcttcgat cgagcttcct tgaagagcga tacgccggcg cccgcgccgc ggagacagct
     6421 cgcagcagac catccgcaaa taaaaaacgc cggcgtcacg cggacgccgg cgttctggtt
     6481 cgatcgcccg gaacgcgggg atcagtcccg gcgcccggcc tgccggcgcg ccctgccgaa
     6541 ggccagcagc ccggcgccga gcagcgcata cgaggccggc tccggaatcg tcgcggccag
     6601 tgccgagacc atctcggctt cgatccaggc gctggcggca gcggtcgggt ggaaggtgtc
     6661 ccagaacacg tactggtccg ggttcgcgca gaccgtgccg gtgttgggcg tgaaggtgat
     6721 gtcgtcgccg gtgtagcagg gtgccgtggt gttggtgaaa ccgtaggcgg ccgggttgcc
     6781 gagcagctcg ttgtaggccg actggatgtc ggccagatgc gcggtgccgg gccgggtctg
     6841 cagcagcgcg agcagcgcgg cgttgaagct cgtcgatgcg tactgcgcga acagcccggc
     6901 cggcgccagc cgcggcgtca gcgacaggtc cggcaggttg gcgacgagga attcatcggc
     6961 gccgagggca agcagcgaac tgatcgccga attgatgttg ctgatcgcga ccgtgacggc
     7021 actgttgatc agcggcagcg cgatcagctg cgcggcggcc ggcgtcgcgc cgccgatcag
     7081 cgcgttctgg taggcggtcg cggcggcgac gaccgcatcc tgcatgtcat tggcgccgat
     7141 ccagatcacg tacagcgcgt ccggatcggc gacgccggcg tgctggctcg cgaaggcacc
     7201 gatctgcgag ctgagccggg cgatcgaatt gagcgccggg ttcgaatgcc cgtaggtgcg
     7261 tgcgccgccc caggcgtagt tgtccccgac gttgccgagc accgagccgg gcagcagcgg
     7321 tgtctgcgta ctggtgaaca ggctgccttc ggccgcgaac cggtcggccc agttcggacc
     7381 gttcgagaaa cggccgttga agtacggcgc cggcggcacc gtgccgcccg agaccagcgc
     7441 gacgttgccg gtatcggaga ggctgtcgcc gaacacgtag aaatccgtat acggcgcggc
     7501 ggcggccgtg gcgctgccga gtgcgagacc ggcagccagc gcaagcccgg gcaggcagcg
     7561 ggtgagaagg gcagatgcgg acatatcagg gcaacctctg gacgggtttc ggacgcagcc
     7621 cctccgctgc ggtcgacggg gcgacgcgac acggccggcg ggggctgcaa ggaagcgcgg
     7681 ggcttcacag gactagcaca gcaacggatg tcctgaagtg ccgcagcccg gtttcggcgg
     7741 cagccaggcc gccggcatgc atctgcgcct gcggtattgt catcaatcgt caaacccgcc
     7801 ccgctgcagt cgcgggaatt acgtgctgca ttgcgccgaa gccgcattgc gactggattt
     7861 tccgatcccg tttccatccg gaatagtcgt aatggacggg tcgtcgatgt ggtatttccc
     7921 gccatgatcg ggccgtggcc gccggtgcgc tgccggcggt ccccccgacg ttcaaacagc
     7981 cgctggaatg ccgccatgag cacgcccccg ttgattgccc gtcaaccggt gtatgacgcc
     8041 cgacaccatg tgctcgcctg ggacattgcc catgccaggg cacagcaccc ggcggccaca
     8101 ccccctgccg agccggactc cgaggcagcc gaagccgcgc acttcgagct gctgaccggt
     8161 gcgctgctgg tcgccggact cgaacggctg atcggcaacg gcttcggctt cctgccggtt
     8221 tcggcgcaca cgctgctcag tccgacgctg cgcctgctgc cgccgctgca ggtggtgctg
     8281 acgctgccgg ccgccggtct ccagtcggtc gacctgccgg cccggctggc gccgctgcag
     8341 cgtgccggtt accgcgtcgc gctcgatggc tgtcgcagcc tcgaacccgg gctcctgccg
     8401 ctgctgccgg atctcgactt cctgcgcctc gatgccgccg cgctcggtgc cgccgcactg
     8461 gcaccgctgc tgcagtcgct gcgcggtgcc ggtttccacg gcgtggcgat cgcgaccggg
     8521 atcgccgatg ccggaaccgc gcaggccgcg ctcgatgccg gcgtgctcgg cctgcaggga
     8581 ccgctgttcc gccggccgca gccggtcgcg gccagcgcac tcgataccgg tcgcgaagcg
     8641 gcgctgacgc tgatcgcggc gctgaaccgc cccgatgccg gcctgcccga gatcagcaac
     8701 ctgatccgcc gcgacgcgac gctgacctac aagctgctgc gctacgtcgg ctcggcctgg
     8761 ttcgggctcg gcgaggtgca ctcggtcgat cacgcgctga tctgcctcgg gcggctgcgg
     8821 ctgcggctgt gggcgacgct ggtcgcgatg accggcgccg ccggcgagcg ccacgacctg
     8881 ctcgaactgg cgctggtgcg cgccggcatg tgccgcgacc tgctgctcgc cgagggcggc
     8941 gatgccgaga ccggcacgct cgccggcctg ctgtcgacgc tcgacctgct gttcggactg
     9001 ccgctggagc gcgtgctcgc cggcctgccg ctcaatcgcg agctgcgtca ggccctgctc
     9061 gaacacgccg gcccggccgg ccgggcgctg gcgctcgcgc tcgcctacga gcgcggcgac
     9121 tgggaggagg tccacgccgc cggccgtgac gagcaggtgt tcgatgccta cttcgctgcg
     9181 ctcgcgcagg cgcacgagac gctggagcag atcggcacca gccactgatc ctccatcccg
     9241 gatcgtccct gaaacacggc gtcgctgcac tgcaaggcgg ccgcccggca cggaaccggc
     9301 tacaagagat tcacgtccct gcgtgaatcc cgcgaatcgc cgaggtgtgc cgatgtccct
     9361 gcccgagtcc gtgaagatcg tcgaggtcgg tccgcgcgac ggcctgcaga acgaggcggc
     9421 cgcggtgccg accgagatca aggtcgagct gatcgagcgc ctgaccgctg ccggcctgcc
     9481 ggcgatcgag gcgaccagtt tcgtgtcgcc gaagtgggtg ccgcagatgg ccgacagcgc
     9541 cgaggtgctg gcgcgcctgc gccggccgcc gggctgcagc tatccggtgc tgacgccgaa
     9601 cctcaagggc ttcgaggccg cgctcgccgc cggggcgcgc gaggtcgcgg tgttcgcggc
     9661 cgcctccgag accttctcgc aacgcaacat caactgttcg atcgccgagt cgctggcgcg
     9721 cttcgagccg ctgctcgccg ccgccgaggc cgccggcgtg cgcgtgcgcg gttacgtctc
     9781 ctgcgtcgcc ggctgcccgt acgaaggcgc ggtggcgccc tcggcggtcg cgcgcgtcgc
     9841 gctcgaactg ctgcgcatgg gctgctacga gatctcgctc ggcgacacca tcggcatcgg
     9901 cacgccggca acgatgctgg cgatgctcga tgccgtcggc ggcgcggtgt cgccggcgcg
     9961 gctggccgtg cactgtcacg acacgcgcgg catgggcatc gccaacgtct acgcctgcgt
    10021 gcaggccggc atcacggtcg tcgatgcctc ggtcgccggc ctcggcggct gcccgtacgc
    10081 accgggcgcg agcggcaacg tcgcgaccga ggatgtcgtc tggctgctcg acggcctcgg
    10141 cgtgcatacc ggcgtcgatc tcgtgaagct cgccgaaacc ggtcagtgga tcagcgaccg
    10201 gctcggccgg ccgaacggct cgcgcgccgg ccgcgcgctg ctcgccctgc gccactgacc
    10261 gcccgccgcc gccccgccgg ccggtcgccg gacttgccac gtggcaagtc gctgccaccg
    10321 gcccggcgcc gcgctgccgc gcctgccgcc ccgtggccgc cgcctgctcc cgattccccg
    10381 atcccgtccc cggagtcccc gcccatgacc gatatcccgc tgtggcagcc cgctccgaag
    10441 cgcatcgccc gcgccaacct gaccgccttc cgcaccgccg tgaacgccgc ccacggcctc
    10501 gacctgccgg attacccggc gctgtacgac tggtcgatcc gcgagcccga agccttctgg
    10561 cgcacgctgt gggactgggc cgacgtgatc ggcgagcgcg gcgagcgcac gctgaccggc
    10621 gccggctcca tgcccggcgc gcagtggttt ccggatgcgc agctgaactt cgccgagaac
    10681 ctgctgcgca ggaacgacga cagcaccgcg atcgtgttcc gcggtgagga caaggtcgaa
    10741 aagcgcctga gctggcgcga gctgaatgcc gcggtgtcgc gcttcgcgca ggcgctgctc
    10801 aacgagcgcg tgcagccggg cgaccgcgtc gcggccttcc tgcccaacct gccggaaacc
    10861 gtcatcggca tgctggcggc aacctcgatc ggcgcggtct ggtcctcgtg ctcgccggat
    10921 ttcggcacgc agggcgtgct cgatcgtttc ggccagatcg aaccggtcgt gctgatcggc
    10981 tgcgacggct actggtacaa cggcaagcgc atcgacaccc tgcacaagct cggcgagatc
    11041 cgcgcgcagc tgccctcggt gcgccggctg gtcgtggtcg gctacctcga tgccatcccc
    11101 gaggtctcgg cgattcccga tgccgtgcgc ctcgatgaat tcatcgcgca ctggccggcg
    11161 cacacgccgg aattcaggcg ccagcccttc agccatccgc tgtacatcat gttcagctcc
    11221 ggcacgaccg gcgtgccgaa gtgcatcgtg cacggcgccg gcggcacgct gctgcagcac
    11281 ctgaaggagc accggctgca caccgacgtg cgccccggcg agcgggtgtt ctactacacg
    11341 acctgcggct ggatgatgtg gaactggctg gtcagcgcgc tggcgagcga ggcgacgctg
    11401 ctgctctacg acggctcgcc gttcgcgccc gacggcaacg tgctgttcga ctacgcgcag
    11461 gccgagcgca tgaacgtgtt cggcacctcg gcgaagtaca tcgacgcctg cgccaaggcc
    11521 ggcatcgcgc cgatcgacac gcacgcgctg cccgagctgc gcgcgatcct ctcgaccggc
    11581 agcccgctgg tgccggagag cttcgattac gtctacgcgc gcgtgaagtc cgacgtgcag
    11641 ctgagctcga tctccggcgg caccgacatc gtctcgtgct tcgcgctcgg caacccggtg
    11701 ctgccggtct ggcgcggcga gctgcagtgc cgcgggctcg ggctgaaggt cgaggtctgg
    11761 aacgacgacg gccagccggt gcgcggcgag aagggcgagc tggtctgcac cgcgcccttc
    11821 ccgtgcatgc cgatcggctt ctggaacgac cccgaaggcg tgaagtaccg ggccgcgtac
    11881 ttcgagcgct tccccggcat ctggtgtcac ggcgactacg tcgaactgac cgcgcacgac
    11941 ggcctgatca tctacggccg ttccgatgcc gtgctgaacc ccggcggcgt gcgcatcggc
    12001 acggccgaga tctaccgcca ggtcgaacag ctcgacgagg tcgtcgagag tctggtgatc
    12061 ggccaggact ggcacggcga cgtgcgcgtc gtgctgttcg tgcacctgcg ccccggcgtc
    12121 gccctcgatg acgcgctgca gaagaagatc cgcgaccaca tccgcgcccg caccacgccg
    12181 cgccacgtgc cggccaagat cgtgcaggtc gaggacatcc cgcgcaccaa gagcggcaag
    12241 atcgtcgaac tggccgtgcg cgacatcgtc cacggccggc cggtgaagaa ccaggaagcc
    12301 ctcgccaacc ccggcgtgct cgcgcagtac gccaactgcg aggaactgac gcgcgactga
    12361 gcacacgccg gcagcctcag ggtttttctc cggatcagtg gtggctcggg gtggcggcgc
    12421 tcgacgaacc gctgctgtcc tcgcccccgc cgttgctgcc cccgccggcc gccacgccga
    12481 tgccgaccgc gagcacggtg gcaatgccga gcggaatcac cggtacgccg agcacggtgg
    12541 cttccaccgc accggccgcc agtccggttt cgccggcacc cgtgccggcg gtctcggggg
    12601 atggcacctg tgacgtctgc tgcgcgagct gctgattccg gacctgctgc tgcggcgagc
    12661 aggactgcgc aacggccttg cgcacggctt cggcacagac acgcgatgct tcgcgcggac
    12721 actgcttgcg cacctgctgg gcaacggcgt cctcgcagct ggtcggcgtc gaactggccg
    12781 atgcggcgtt gctgttgccc tgttgctggg ccggggcggg cagcgcggaa atcatcagga
    12841 tgccggccag ggccggggcg agcgagcgct gcagggtgaa tctcctgagc atggcggaat
    12901 actccgtgcg tgaaaggaag atgatggctg ccaagagacc ggatcggagc aaggatcagg
    12961 aaccgatcaa aaagcaagta ccggtccata ttaacaatct gtcaccagtt ttcacgccac
    13021 ccgtcacgca cgcattgctg cccatgtccc ctgcacccct gtcctgccac cggcacccgt
    13081 ttcctcaggc ccggtgaccg gtgtgcacca cgccggtgcc ctccggttca gccgagcgcc
    13141 gccaggcggg cgagcagcgt cgcccggccg gcatcgaagc ggttgccgat ctgctgcagt
    13201 tgcgcgctca ggcccgcagg cagtggcacg gcatcggccg atggcggcgc ttcatcgttt
    13261 gcaggcgcgt cgatctcgat ctgcagcacc cgctcggtga ccgcgccgcc ggcgtcacgc
    13321 tcccggatgc gcagttcgat ggttccgcgc acgccatccg gcacctgacc gctgatgcgg
    13381 ccggtcgcgg gatcgaaatt catccacgcc ggcagcgcct cgccggtgct cagccgtacc
    13441 tcacgcgtac cgccggccgt cagcggcggc agcgagaacc cgaagctgct gccggcccgc
    13501 aggctttccg gcgccagcgc cggccgctcg aagcccgtcc ccgtgccgtt atccggccgc
    13561 aagccaggta tgagcagcgg cggcggggcg ttcggggtgc tcaggggcgg gatggggttc
    13621 gaataggtct gcaaggtatt gcgaacctgc tcgaagccga cgccgggcgc cggcgggcgg
    13681 accatcagcg tgtcattgcc gtagaccgca tcgcgcacgg catcgtcgct gccgatgccg
    13741 gaatcgccgg tgaccgccgc gagcacgaga ttcggctgca cggcggaggt cggggcaaag
    13801 gtcgttggcg caggcgccga ggtcgggctg agcggcgcag tggagttgac cggcaccgga
    13861 ctgcgggttt cgagcacggt cacctgtacc gtgccgatgg ccgtggcgcc gttgcgatcg
    13921 gtcacggtgt accggaaagc atcctggccg gagaaaccga attccggttc gtaataaatc
    13981 cggccgtcgg cttcgatgcg aaccgtaccg tgagccggcg attcgacggc agtgacgcgc
    14041 agcgcgtcgt ccgcatcgac atcgctgtcg ttggcgagca cgttgatgac gattgccgtt
    14101 tccatgtcgg tggtcacggc atcgttcgcg gcaaccggcg cgtcattggt attgag
//
LOCUS       DAOQIE010000056        13882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2119_length_13882_cov_7.675345, whole genome shotgun sequence.
ACCESSION   DAOQIE010000056 DAOQIE010000000
VERSION     DAOQIE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2119_length_13882_cov_7.675345"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PK144_05360"
     CDS             complement(<1..1460)
                     /locus_tag="PK144_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNF65187.1"
                     /translation="MASSNIKLLSFSGNVRILHLSWIAFFISFVVWFNHAPLLASIRE
                     TFQLTDGQVKALLTLNVALTIPVRILIGMLVDKFGPRIMYSVLLAVCGGLCFFFALAN
                     SYEQLALARLLLGCVGAGFVIGIRLISEWFPAKQVGFAEGIYGGWGNFGSAAAALSLP
                     VLAHAFGGPDAWRWAVGVTGVISIIYSVIFYALASDTPKGSTYFRPKKLGAMEVTSKS
                     DFWLYVFLYVPMTVALLVLTWKLSPAGFKLIDQTTTNVVYVALLALFAWNVWQVIDVN
                     RNILAGEVVPQIHRYKFKQVAILSGAYAIAFGSEIAVVSMLPLYFKDMYNLTLIQAGL
                     VGSSFAIMNLFARPAGGLISDRFGRKRTMTIVMIGITFGYFGMSFMGGMPLWAAVTVT
                     VINSFFVQAGCGAVYGIIPLIKRRLTGQIAGMSGAYGNVGSVVFLTVLSFVTPSTFFM
                     VIAASALVVLGLVQFLDEPSGQMAEIGFSLYTELLER"
     gene            1463..1867
                     /locus_tag="PK144_05365"
     CDS             1463..1867
                     /locus_tag="PK144_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65188.1"
                     /translation="MNPLEEKRYHQWQFAGQLEKNQSGCGVREFRHGTHIGVMHSGSA
                     GFCASTVHLCQSRAKKRFATALDRCESGVWRNTTKEVFPANSIWLAVFALNQGVGHGF
                     EPNRCITRMQSHGHRKVSCLHLPKKQLLVSIK"
     gene            1968..4406
                     /gene="nirB"
                     /locus_tag="PK144_05370"
     CDS             1968..4406
                     /gene="nirB"
                     /locus_tag="PK144_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNF65189.1"
                     /translation="MKERLVLIGNGMAGMRTIEELQKVAPQMYDITVFGAEPHGNYNR
                     IMLSPVLAGEKTIPEIMINDLDWYEQNGVTLHAGKDKEIVEINRGRREVRAADGTTVK
                     YDRLLIATGSNPFILPLPGNDLPGVIGFRDIKDVDAMLHAAKTGKHAVVIGGGLLGLE
                     AANGLMKQGLQVTVVHVLDTLMERQLDPFAADMLRKTLEERGLRFLMGAHTAAILGDD
                     KVRGVRFKDGLEVPADIVVMAVGIRPNIELAKKSGIHCERGIVVNDTMQTYDPRVYSV
                     GECVQHRGVCYGLVAPLWEQAKVCASHLAQHGVGRYEGSVTSTKLKVTGIDLFSAGNF
                     NGGPDCEELVLRDPARGVYKKLVVKDNRVQGAVLYGDTVDGTWYFQLLRENTDITAFR
                     ETVLFGHAHMADAGREGHSVMSMSDDAEICGCNGVCKGTIVKAITEKKLFTVDDVRAH
                     TKASASCGSCTGLVEQILAATVGNYDATPKEKPMCKCTDHPHDVVRKAIRDEHLTSLE
                     SVFKVLNWKTANGCASCRPALNYYLISTWPAEAKDDYQSRFINERVHANIQKDGTYSV
                     VPRMWGGLTNARELRAIADAADKYNVRTVKVTGGQRIDLFGIRKEDLPKIWADLNAAG
                     MVSGHAYGKSLRTVKTCVGSDWCRFGTQDSTGLGVKIEQMTWGSWTPHKFKMAVSGCP
                     RNCAEATIKDFGVVCVDSGYEAHVGGNGGIHVRGTDLLCKLETEEQVLEYCGAFMQLY
                     REEGRYLERTAPWIERVGLEYVKAKIVEDEEGRKALYARFLESQKYAQTDPWAERASA
                     GVDAHEYAPVATIG"
     gene            4539..4892
                     /gene="nirD"
                     /locus_tag="PK144_05375"
     CDS             4539..4892
                     /gene="nirD"
                     /locus_tag="PK144_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNF65190.1"
                     /translation="MSDWHDIGTVEDIPRLGARVVQSPQGNIAVFRTAEDEIFALRDR
                     CPHKAGPLSQGIVHGKRVTCPLHSWTLELGTGEAIAPDVGCAARFPVKVEGGRILLQV
                     SAVAAVSDHSAGTLA"
     gene            5041..7728
                     /locus_tag="PK144_05380"
     CDS             5041..7728
                     /locus_tag="PK144_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNF65191.1"
                     /translation="MPAEAPEVRTTCPYCGVGCGVIAYRTDDGTIAVRGDEQHPANFG
                     RLCSKGSALAETLALEDRLLHPEIDGQRVGWDAALDAVAEGFRRTIAEHGPDAVALYV
                     SGQLLTEDYYVANKLMKGCIGTSNIDTNSRLCMSSSVAGHKRAFGTDTVPGTYEDLEQ
                     ADLLILVGSNLAWCHPVLYQRILAARQARGGKPKLIVIDPRRTASCDQAEQHLAIAPN
                     TDTVLFNGLLVHLHRQDAVDLDYLDAHTDGYLAALRAAREIAPSIPEVARRCGLDEEA
                     VANFYGDFTRTKKVVTVYSQGVNQSAHGTDKVNAIINCHLATGRIGKPGCGPFSVTGQ
                     PNAMGGREVGGLANQLAAHMDFAPADVERVARFWQAPNIARGPGLKAVELFRAVAEGR
                     IKAIWIMGTNPVVSLPEADAVREALMNCPLVVVSDAVRVTDTTACADILLPAATWGEK
                     DGTVTNSERRISRQRAFLDLPGEARPDWWALAEVGRRLGFTANLDFAGPAAVFREFAA
                     LSGYENAAWGGEGDSRRDFDISALAGVADADYDTIEPFRWPRPAAGDGAERFFATGGY
                     YTPDRRARMIAVGERASVNAPDETFPLVLNTGRVRDHWHTMTRTAKTARLSAHIPEPY
                     AEIHPVDAAVLGIRDEALVRVVSRWGEVIVRAKLAREQRPGSVFVPMHWCTPYASRGR
                     IDAVVNPATDPFSGQPESKHTPVRVMAYKPAWHGFVLSRDPLTLPDDLDYVVRVRGDG
                     LWRYEIASEQPVADWSAWAHELLHEPPGEGWIEFEDRSLGRYRGAAMHDGRLVACAFV
                     SPGHELPPRDWLAGLFERPELAAPERASLLAGRPGKGQPDVGRIVCACFRVGVNTLLS
                     AIRERKLTTPEAIGAALRAGTNCGSCVPEIRKLIADNAS"
     gene            7874..8320
                     /locus_tag="PK144_05385"
     CDS             7874..8320
                     /locus_tag="PK144_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNF65192.1"
                     /translation="MHKRITRAAFAAALALAVQIAGAAEFGTADEAKAMLEKAVAALK
                     ADKAAALSQFAKGEGGFKDRDLYPFCGGPDGNFTAHPTLTGKSLRDLKDKSGKPLGEE
                     IYSTAQEGRISEVNYMWPRTGGTDPVQKVSYVTRVGDQVCAVGYYK"
     gene            complement(8479..9453)
                     /gene="cysB"
                     /locus_tag="PK144_05390"
     CDS             complement(8479..9453)
                     /gene="cysB"
                     /locus_tag="PK144_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNF65193.1"
                     /translation="MKLQQLRYLCAIAKNQLNISAAAESLFTSQPGVSKQVRLLEDEL
                     GVELFNRNGKHLTHITPVGRHIIARAEAILREVRNIQILAEEFRDDARGTLSIATTHT
                     QARYALPPIIKTFRARYPEVVLQLHQGSPTQIAEMAASGAVDFAIATEAIEEFHDLVM
                     MPCYLWNRCALVPQGHPLTGSTPLTIEALARHPLVTYVFGFTGRSQLDEAFKAARLHP
                     EVAFTATDADVIKTYVRIGLGVGIIARMAWDAEADRDLVALDASHLFAPNTTKIGFRR
                     GTFLRQFQYDFIQLFAPHLTAQVVQTAEASESQEALDELFASLELPEY"
     gene            10077..11024
                     /locus_tag="PK144_05395"
     CDS             10077..11024
                     /locus_tag="PK144_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65194.1"
                     /translation="MSDHPPVDAVLRIELLERFRQLHESLDALATAIEADQDRAAWLD
                     EAPVTEIRTRAATLIRDIWYGDEVEDARMTVTSIGLIGCCAETLTLAQAANAAKDAFK
                     TVVLQFRGKTSERNRALAEMLETWHARDPDLARALSAGGIGRVHLVQAYRHVPVLDET
                     PVRVGFSYASSARAIRALTRDQALELVEALPDTHAARAVYRQMLSQYPDSERFAQMRN
                     LAPNLVANLVFRDGYGEEERLRRGVMVRVRTEQVRRKLLHVHLPILFPLAAGAPLPAH
                     PARGPQVRSAASRLLRSDRRIDGPPLIPALRLYRYREPD"
     gene            complement(11066..12820)
                     /locus_tag="PK144_05400"
     CDS             complement(11066..12820)
                     /locus_tag="PK144_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF65195.1"
                     /translation="MSKRRADFAIFNNSMMGFPKQNIPYEYSCCFIRHIFWYLSYLLI
                     YPSNMLEHHSSDEPTQALLLALLDLWQFVARPAAAGRLDAVLPDITRILGRALPVTAL
                     ELLIPDAGNQQLRGWRCEAADETRSSRCPLPAAELSALTSWCAASTIETTNGRSRPRW
                     LKALPTLSASRADLVSGAGPMAALGLAGSAGEHSALALLHLRAGAGIGPGLRGCLARI
                     AAGLRTLLDWQDVRSELSGLHELVSQLRDREPVRSTETIVGADGGLRPVLERVRLVAA
                     TDVPVLILGETGSGKEVIARAIHQGSPRTGQAFVRVNCGALPPDLIDTELFGHERGAF
                     TGAAAARRGWFEQADRGTLFLDEIGELPLAAQVRLLRVLQDGTLNRIGSETPLNVNVR
                     VVAATHRDLPSMVLAGRFREDLWYRLATFPVLLPPLRERKADFADLAAHFARRAAHRF
                     GFPVVQPTGTDVALLSAYDWPGNVREFAAVIDRALLLGEGRSLDVATALGAPRVQALQ
                     PVAAGLSPSPVPMAGAPGTLATLDEAMRSHIARALEMTHGRLEGRHGAALLLGINPHT
                     LRARMRKLGLDPAQFRPH"
     gene            12859..13881
                     /locus_tag="PK144_05405"
     CDS             12859..13881
                     /locus_tag="PK144_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF65196.1"
                     /translation="MTLLPLRAALFGWLSACCLAGSGNVNAADLELLNVSYDPTRELY
                     QAYNAAFVREWQAGHPGDTLTIRQSHGGSGKQARAVIDGLAADVVTLALAGDIDAVAR
                     SGLIAGDWQKRLPHASTPYYSTIVFLVRKGNPKGLKDWGDLARPGVAVITPNPKTSGG
                     ARWNYLALWGWALRQPGGSEDSARALLEKVFRNVPVLDTGARGSTTTFVQRGIGDVLL
                     AWENEALLALKEYGSDAFEIVVPKTSIRAEPPVAVVDKVVERRGTQTLADAYLRGLYE
                     PAAQRIAAEHGYRPTDAAVAAEFRARFPQIELFGIDDFGGWSAVQSKHFADGGEFDRL
                     YQPGSR"
BASE COUNT         2529 a         4594 c         4518 g         2241 t
ORIGIN      
        1 cgctcgagca gttccgtgta gagcgagaat ccgatctcgg ccatctggcc gctcggctcg
       61 tcgaggaact gcacgaggcc gagcacgacc agcgcgctgg cggcgatgac catgaagaag
      121 gtcgacggcg tcacgaagga cagcacggtc aggaacacga cgctgccgac gttgccgtag
      181 gcaccgctca tgccggcgat ctggccggtc aggcgacgct tgatcagcgg gatgatgccg
      241 tagaccgcgc cgcaaccggc ctgcacgaag aacgagttga tgacggtcac cgtcaccgcc
      301 gcccacagcg gcatgccgcc catgaagctc atgccgaagt agccgaaggt gatgccgatc
      361 atgacgatgg tcatcgtgcg cttgcggccg aagcggtcgg agatcaggcc accggccggg
      421 cgcgcgaaca ggttcatgat cgcgaagctc gaaccgacca gaccggcctg gatcagcgtc
      481 aggttgtaca tgtccttgaa gtacagcggc agcatcgaca ccacggcgat ctcggagccg
      541 aaggcgatcg cgtaggcacc cgacaggatc gcgacctgct tgaacttgta gcggtggatc
      601 tgcggcacga cctcaccggc gaggatgttg cggttcacgt caatcacctg ccagacgttc
      661 cacgcgaaca gcgcgagcag cgcgacgtag acgacgttgg tcgtggtctg gtcgatcagc
      721 ttgaagccgg ccggcgacag cttccaggtc agcaccagca gcgccacggt catcggcacg
      781 tacaggaaca catacagcca gaagtcgctc ttgctggtga cttccatcgc gccgagcttc
      841 ttcgggcgga aataggtcga gcctttcggg gtgtcgctgg ccagcgcgta gaagatcacc
      901 gagtagatga tcgagatcac gccggtgacg ccgaccgccc agcgccaggc atccgggccg
      961 ccgaacgcgt gtgcgagcac cggcaggctc agtgccgctg cggccgaacc gaagttgccc
     1021 cagccgccgt agatgccttc ggcgaagccg acctgcttgg ccgggaacca ctccgagatc
     1081 aggcggatgc cgatgacgaa gccggcaccg acgcagccga gcagcaggcg cgccagcgcc
     1141 agctgctcat agctgttggc gagcgcgaag aagaagcaca ggccgccgca gaccgccagc
     1201 agcaccgaat acatgatgcg cgggccgaac ttgtcgacca gcatgccgat caggatgcgc
     1261 accgggatgg tcagcgcgac gttcagcgtc agcagcgcct tcacctggcc gtccgtgagc
     1321 tggaatgttt cgcggatcga cgcgagcagc ggcgcgtggt tgaaccacac cacgaaactg
     1381 atgaaaaacg cgatccagct caggtgcagg atgcgcacgt tgccgctgaa cgacagcagc
     1441 ttgatgttcg acgatgccat cggtgaatcc tctcgaagaa aagcggtatc accaatggca
     1501 attcgcaggc caacttgaaa aaaaccagtc cggctgcggg gttcgggaat tcaggcacgg
     1561 cacgcatatt ggcgtaatgc acagcggcag tgccggtttt tgcgccagca cggtgcattt
     1621 gtgccaaagc agggcaaaaa aacgcttcgc gaccgcactt gatcggtgcg aatcaggggt
     1681 atggcgtaat accaccaaag aggtattccc agctaattca atatggttag cggtttttgc
     1741 actgaatcaa ggcgtggggc acggttttga accgaaccgg tgcattacca gaatgcaatc
     1801 ccacgggcac cgaaaggttt cttgccttca tcttccgaaa aaacagttac ttgtatccat
     1861 taaatgaaaa tggcacggca tatgcttaac ggcggctcag gctaaggcat tcacagccgc
     1921 acgccgaatc ggctctgcgt caaagattct tccgcgagga aatgttcatg aaagaaagac
     1981 tggtcctgat cggcaacggc atggcgggca tgcgcacgat cgaggaactg cagaaggtcg
     2041 cgccgcagat gtatgacatc acggtgttcg gcgccgagcc gcatggcaat tacaaccgca
     2101 tcatgctgtc tccggtgctc gccggcgaaa agaccattcc ggaaatcatg atcaatgatc
     2161 tggactggta cgagcagaat ggcgtcacgc tgcacgccgg caaggacaag gaaatcgtcg
     2221 agatcaaccg cggccggcgc gaagtgcgcg ctgccgacgg cacgacggtg aaatacgacc
     2281 gcctgctgat cgccaccggt tccaatccgt tcatcctgcc gctgccgggc aatgacctgc
     2341 cgggcgtcat cggcttccgt gacatcaagg atgtcgatgc gatgctgcac gccgcgaaga
     2401 ccggcaagca cgccgtcgtc atcggtggcg gcctgctcgg tctcgaagcc gccaacggcc
     2461 tgatgaagca gggcctgcag gtcacggtcg tgcacgtgct cgacacgctg atggagcgcc
     2521 agctcgaccc gttcgcggcc gacatgctgc gcaagaccct ggaggagcgc ggcctgcgct
     2581 tcctgatggg cgcgcacacc gccgcgatcc tcggtgacga caaggtccgc ggcgtgcgct
     2641 tcaaggacgg cctcgaagtg ccggccgaca tcgtcgtcat ggccgtcggc atccgcccga
     2701 acatcgagct cgcgaagaag tccggcatcc actgcgagcg cggcatcgtc gtcaacgaca
     2761 cgatgcagac ctacgacccg cgcgtgtact cggtcggcga atgcgtgcag caccgcggcg
     2821 tctgctacgg cctggtcgcg ccgctgtggg agcaggccaa ggtctgcgcc agccacctcg
     2881 cgcagcacgg cgtcggccgc tacgagggct cggtgacgag caccaagctc aaggtcaccg
     2941 gcatcgacct gttctcggcc ggcaacttca acggcggccc ggactgcgag gaactcgtcc
     3001 tgcgcgaccc ggcccgcggc gtctacaaga agctggtcgt caaggacaac cgcgtgcagg
     3061 gcgccgtgct ctacggcgat accgtcgacg gcacctggta cttccagctg ctgcgcgaga
     3121 acaccgacat caccgccttc cgcgagaccg tgctgttcgg tcacgcgcac atggccgatg
     3181 ccggccgcga aggccacagc gtcatgtcga tgtccgacga cgccgagatc tgcggctgca
     3241 acggcgtctg caagggcacg atcgtcaagg cgatcaccga gaagaagctg ttcacggtcg
     3301 atgacgtgcg cgcccacacc aaggcctcgg cctcgtgcgg ttcgtgcacc ggtctggtcg
     3361 agcagatcct cgcggccacc gtcggcaact acgacgcgac gccgaaggaa aagccgatgt
     3421 gcaagtgcac ggatcacccg cacgacgtcg tgcgcaaggc gatccgcgac gagcacctga
     3481 cctcgctcga atccgtgttc aaggtgctga actggaagac cgccaacggc tgcgcttcct
     3541 gccgcccggc gctgaattac tacctgatct cgacctggcc ggccgaggcg aaggacgact
     3601 accagtcgcg cttcatcaac gagcgcgtgc acgccaacat ccagaaggac ggcacctact
     3661 cggtcgtgcc gcgcatgtgg ggcggcctga ccaacgccag ggaactgcgc gcgattgccg
     3721 atgccgccga caagtacaac gtgcgcacgg tcaaggtcac cggtggccag cgcatcgacc
     3781 tgttcggcat ccgcaaggaa gatctgccga agatctgggc cgatctcaac gccgccggca
     3841 tggtctcggg tcacgcttac ggcaagagcc tgcgcaccgt gaagacctgc gtcggttccg
     3901 actggtgccg cttcggcacg caggattcga ccgggctcgg cgtcaagatc gagcagatga
     3961 cctggggctc gtggacgccg cacaagttca agatggccgt ctccggctgc ccgcgcaact
     4021 gcgccgaggc gacgatcaag gacttcggcg tggtctgcgt cgactccggc tacgaggccc
     4081 acgtcggcgg caacggcggc atccacgtgc gcggcaccga tctgctctgc aagctcgaaa
     4141 ccgaggagca ggtgctcgaa tactgcggcg ccttcatgca gctgtaccgc gaggaaggcc
     4201 gctatctgga gcgcaccgcg ccgtggatcg agcgcgtcgg cctcgaatac gtgaaggcca
     4261 agatcgtcga ggacgaggaa ggccgcaagg cgctgtacgc gcgcttcctg gaatcccaga
     4321 agtacgcgca gaccgatccg tgggcggagc gcgccagcgc cggcgtcgat gcgcacgagt
     4381 acgcgccggt cgcgacgatc ggctgatcat cggccgatcc gcttcgtgag ttcgcgagat
     4441 aaatttttat accatcatca atactgaaag ccgggtcact ccgggtgcat ggatccacat
     4501 cagcacccgg gatgaccacg gtccggagca gagtccccat gagcgactgg cacgacatcg
     4561 gtaccgtcga agacattccg cgcctcggtg cgcgcgtggt gcagagcccg cagggcaaca
     4621 tcgccgtgtt ccgcacggcc gaggatgaaa tcttcgcgct gcgcgaccgc tgcccgcaca
     4681 aggccggtcc gctgtcgcag ggcatcgtgc acggcaagcg cgtgacctgc ccgctgcaca
     4741 gctggacgct ggagctcggc accggcgagg cgatcgcgcc cgacgtcggc tgcgcggccc
     4801 gcttcccggt gaaggtggag ggcggccgca tcctgctgca ggtcagcgcc gtcgccgccg
     4861 tgtccgatca cagcgcgggc acgctggcct gaaagcccgc ggggcggcgt tgtccgccgt
     4921 cccgcgctga tcctcaccgc cggggccgcg cgtcccggca cgcatcaagg gttgcgcacg
     4981 cgctggcgtg cgtgaccggc gcccgttccc gtcacggcga tcgttccagg agattcccgc
     5041 atgcctgccg aggcccccga agtccgcacg acctgtcctt attgcggtgt cggctgcggc
     5101 gtcatcgcct accgcacgga tgacggcacg atcgccgtgc gcggcgacga gcagcatccg
     5161 gccaacttcg gacggctttg ctcgaagggt tcggcgctgg ccgaaacgct cgcgctcgaa
     5221 gaccgtctgc tgcatccgga aatcgacggc cagcgggtcg gatgggatgc ggcgctcgat
     5281 gcggtcgccg agggcttccg gcgcacgatc gccgagcacg gcccggatgc cgtggcgctg
     5341 tacgtctccg gccagctgct gaccgaggac tactacgtcg ccaacaagct gatgaagggc
     5401 tgcatcggca cctcgaacat cgacaccaat tcgcggctgt gcatgtcctc gtcggtcgcc
     5461 ggccacaagc gcgcgttcgg caccgacacc gtgcccggca cctacgagga tctcgaacag
     5521 gccgacctgc tgatcctcgt cggctcgaac ctcgcctggt gtcacccggt gctgtaccag
     5581 cgcatcctcg ccgcccgcca ggcgcgcggc ggcaagccga agctgatcgt catcgacccg
     5641 cgccgcaccg cgagctgcga tcaggccgag cagcatctgg cgatcgcgcc gaacaccgac
     5701 accgtgctgt tcaacggcct gctcgtgcac ctgcaccggc aggatgccgt cgatctcgac
     5761 tacctcgatg cgcacaccga cggttatctg gcggcgctgc gcgcagcgcg cgagattgcc
     5821 ccgtcgatcc ccgaggtcgc gcgccgctgc ggtctcgacg aggaggcggt cgcgaacttc
     5881 tacggcgatt tcacgcgcac gaagaaggtc gtcaccgtct attcgcaggg cgtcaaccag
     5941 tcggcgcacg gcaccgacaa ggtcaacgcc atcatcaact gccatctggc gaccggacgc
     6001 atcggcaagc ccggctgcgg tccgttctcg gtcaccggcc agccgaacgc gatgggcgga
     6061 cgcgaggtcg gcggcctcgc caaccagctc gccgcgcaca tggacttcgc tccggccgat
     6121 gtcgagcgcg tcgcccgctt ctggcaggcg ccgaacatcg cgcgcggccc gggcctgaag
     6181 gcggtcgagc tgttccgcgc cgtcgccgag ggcaggatca aggcgatctg gatcatgggc
     6241 acgaatccgg tggtcagcct gccggaggcc gatgcagtgc gcgaggcgct gatgaactgc
     6301 ccgctggtcg tcgtctccga tgccgtgcgc gtcaccgaca ccacggcctg cgccgacatc
     6361 ctgctgccgg ccgcaacctg gggcgagaag gacggcacgg tcaccaattc cgaacgccgc
     6421 atctcgcgcc agcgcgcctt cctggatctg cccggcgaag cgcgccccga ctggtgggcg
     6481 cttgccgaag tcggccgccg gctcggattc acggcgaatc tcgatttcgc cggtccggcc
     6541 gcagtgttcc gcgagttcgc ggcgctgtcc ggctacgaga acgccgcctg gggcggcgag
     6601 ggcgacagcc ggcgcgattt cgacatctcg gccctggccg gcgtcgccga tgccgactac
     6661 gacacgatcg aaccgttccg ctggccgcgc ccggccgccg gcgacggcgc cgagcgcttc
     6721 ttcgcaaccg gcggctatta cacgcccgat cgccgggcac gcatgatcgc ggtcggcgag
     6781 cgggcgtcgg tgaacgctcc ggacgaaacc tttccgctgg tgctcaacac cggtcgcgtg
     6841 cgcgatcact ggcacacgat gacgcgcacg gccaagaccg cacgtctgtc ggcgcacatc
     6901 ccggaaccgt atgccgaaat ccacccggtc gatgccgccg tgctcggcat ccgcgacgag
     6961 gcactggtgc gcgtcgtcag ccgctggggc gaggtgatcg tgcgcgccaa gctcgcacgc
     7021 gagcagcgcc cgggctcggt gttcgtgccg atgcactggt gcacgccgta cgcgagtcgc
     7081 ggccgcatcg atgccgtcgt caatccggcc accgatccgt tctccggcca gcccgaatcc
     7141 aagcacacgc cggtgcgcgt gatggcgtac aagccggcct ggcacggctt cgtgctgtcg
     7201 cgcgacccgc tgacgctgcc cgacgatctc gactacgtcg tgcgcgtgcg cggggatgga
     7261 ctctggcgct acgagatcgc ctcggaacag ccggtcgccg actggagcgc ctgggcccac
     7321 gaactgctgc acgagccgcc gggcgagggc tggatcgagt tcgaggaccg ctcgctcggc
     7381 cgttaccgcg gtgcggcgat gcacgacggc cgtctggtcg cctgcgcctt cgtctcgccc
     7441 ggccacgaac tgccgccgcg cgactggctg gccggcctgt tcgagcgccc ggaactcgcc
     7501 gccccggagc gcgcgagcct gctcgccggc cgccccggca agggccagcc cgatgtcggg
     7561 cgcatcgtct gcgcctgctt ccgcgtcggc gtcaacacgc tgctgtcggc gatccgcgag
     7621 cgcaagctga cgacaccgga agcgatcggt gccgcgctgc gtgccggcac caactgtggt
     7681 tcctgcgtgc ccgagatccg caagctgatc gccgacaacg cctcctgagc agcctggccc
     7741 gggatttccc atgcttgcct catgggaaat gacgctgcta tacctcctct gccttcacga
     7801 acgatgaggg tggccggccg atgcctttac cggcaccggc cggcgaacat aaatcaggca
     7861 gaggagagac ttcatgcaca agcgcatcac gcgcgcagca ttcgccgcgg ccctcgcgct
     7921 cgccgtccag atcgccgggg ccgccgagtt cggcaccgcc gacgaggcga aggcgatgct
     7981 cgaaaaggcc gtggccgccc tgaaggccga caaggccgcc gccctgtcgc agttcgccaa
     8041 aggggagggc ggcttcaagg atcgtgacct gtatccgttc tgcggcggtc cggacggcaa
     8101 cttcaccgca catccgacct tgaccggcaa gagcctgcgt gacctgaagg acaagtccgg
     8161 caagccgctc ggcgaggaaa tctactcgac ggcccaggaa ggccggatca gcgaagtcaa
     8221 ctacatgtgg ccgcgtaccg gcggcaccga tcccgtgcag aaggtcagtt acgtgaccag
     8281 ggtcggcgat caggtctgcg cggtcggata ttacaagtga tgccttgggc atgagccgca
     8341 gtctgcaatg tgcagacggg cggacgaaac ccgacaaggc gccaaacggc gccttgttta
     8401 tttataatca aataattttt tataccatca tattttacac tgatcacaaa tccgattttg
     8461 ttccttccac cacacccatc aatattccgg caactccaga gaagcaaaca gctcatccag
     8521 cgcctcctgg gattcggaag cttcggcggt ctgcacgacc tgcgccgtca gatgcggtgc
     8581 aaacagctgg atgaagtcgt actggaactg gcgcaggaag gtgccgcgcc ggaaaccgat
     8641 cttggttgta ttgggcgcaa acagatggct ggcatcgagc gcgaccagat cccggtcagc
     8701 ttcggcatcc caggccatgc gcgcaatgat gccgacgcca aggccgatgc ggacatacgt
     8761 cttgatcaca tcggcatcgg tggccgtgaa cgcaacttcc ggatgcaggc gtgcggcctt
     8821 gaacgcctcg tcgagttgcg aacggccggt gaatccgaac acgtaggtca ccagcggatg
     8881 ccgggcgagg gcctcgatcg tcagtggcgt cgatccggtc agcggatggc cttgcggcac
     8941 cagcgcacag cggttccaga ggtaacaggg catcatcacc agatcatgga attcctcgat
     9001 cgcctcggtc gcaatcgcaa aatcgacggc tccggaggcg gccatctcgg caatctgggt
     9061 cggcgagccc tgatgcagct gcagcacgac ttccgggtaa cgggcccgga aggtcttgat
     9121 gatcggcggc agcgcatagc gggcctgggt atgcgtggtc gcgatcgaca gcgtgccacg
     9181 ggcatcgtcg cggaattcct cggcgaggat ctggatgttg cggacttcgc gcaggatcgc
     9241 ctcggcgcgg gcgatgatgt gccggccgac cggggtgatg tgggtcaggt gcttgccgtt
     9301 gcggttgaac agctcgaccc cgagctcgtc ctcgagcagt cgcacctgct tggatacccc
     9361 gggctgcgac gtgaacagac tctcggctgc ggcagagatg ttgagctgat tcttggcgat
     9421 tgcgcacagg tagcgcagct gctgcagttt cactgacccg acctcatcgg catcgcatat
     9481 tccggagaag cataacgcag agcggatgag ttcgcgttcc ggctggatgc gccacctgct
     9541 ggaggaaggc agccacttcg gcgcttgacg acgggcgttg gcggtttgcg ttcatgggtg
     9601 tcgggacaca ggcatcacca gcccggaaga cggtcggttc aaggcttcag ggagccatga
     9661 cacggactgt atcttgagcc taataatgta tattatgtca aatacgctag agatgtcatg
     9721 aaacccgatt ttcctcgatg ccgacgaccg ggatgctgac gaccggatca tgttcccctt
     9781 tccaccagga gcaccctgct caccttccgg ttgttttcga gccgcctcct gctttttcga
     9841 acgccttcaa cccctgtgca tccgatcgcc ccttgcactg ctcggtccga tgatcttcca
     9901 gaggctggaa tccatgtgga tgcgaacgtc gatgcaatgg ctggatctgc gtcggatttc
     9961 ttttgctgac gttgtatgtg tcttgtctgg aatttgggag gatttctgaa tttcgggtga
    10021 ttaaaaatat gatgatataa tttcacacca tcaagctcct gccgcagggt gtgcccatgt
    10081 ctgaccatcc gcccgtcgat gcggtcctgc gtatcgaact gctggagcga ttccggcaac
    10141 tgcatgaatc tctggatgct ctggcaactg ccatcgaagc tgaccaggat cgggccgcgt
    10201 ggctggatga ggcaccggtc accgaaatcc gcacgcgcgc agcgacgctg atccgggata
    10261 tctggtatgg cgatgaagtc gaggatgctc gcatgacggt gacctcgatc gggctgatcg
    10321 gctgctgcgc cgaaacgctg acgctcgcgc aggctgcaaa tgcagccaag gatgccttca
    10381 agaccgtcgt gctgcagttt cgcggcaaga ccagcgaacg taaccgggca ctggccgaga
    10441 tgctcgaaac ctggcatgcc cgcgatcccg atctggcccg tgccctcagc gccggcggca
    10501 tcggccgcgt gcatctggtc caggcgtacc ggcatgtgcc ggtgctcgat gaaacaccgg
    10561 tgcgggtcgg tttttcctat gcgagcagtg cgcgggcaat ccgggcgctg acgcgcgatc
    10621 aggcactgga actggtcgag gccttgccgg acacacacgc cgcccgcgcc gtgtaccggc
    10681 agatgctcag ccagtatccg gacagcgagc gctttgcgca gatgcgcaac ctggcgccga
    10741 atctggtcgc caatctcgtg ttccgggatg gttatggcga ggaagaacgc ctgcggcgcg
    10801 gcgtaatggt gcgggtccgg accgagcagg tacgccgcaa gctgctgcac gtgcatctgc
    10861 cgatcctgtt cccgctcgca gcaggcgctc ccctgcccgc tcacccggcc cgcggcccgc
    10921 aggttcgcag cgctgccagc cgcctgctgc gctcggaccg gcgcatcgac ggtccgccgc
    10981 tgatcccggc gctgcggctg taccggtatc gcgagccgga ctgagcaccg gtcgtgtcga
    11041 tcagccgtac cggaccatgc cggcgtcagt gcggacggaa ctgcgcaggg tcgagaccca
    11101 gcttgcgcat gcgggcgcga agcgtgtgcg gattgatgcc gagcagcagt gccgcgccat
    11161 ggcgaccttc gaggcggcca tgcgtcatct ccagtgcgcg ggcgatgtgg gagcgcatcg
    11221 cctcgtcgag tgtcgccagt gtcccgggcg caccggccat cgggacagga gacggggaca
    11281 ggccggcagc aaccggctgc agggcctgca cccgcggtgc gcccagcgca gtggccacat
    11341 ccagcgacct cccttcgccg agcagcaggg cccgatcgat gacggcggcg aattcccgga
    11401 cattgccggg ccagtcatag gcgctcagca gggcgacatc ggtcccggtc ggctgcacga
    11461 ccggaaagcc aaagcggtgc gcggcgcggc gggcgaaatg ggccgccaga tcggcgaagt
    11521 ccgccttgcg ctcccgcaag ggcggcagca gcaccggaaa cgttgcgagc cggtaccaga
    11581 gatcttcgcg aaagcgcccc gccagcacca tggaaggcag atcacgatgc gtggcggcga
    11641 ccacgcgcac gttgacgttt agcggggttt cactgccgat gcggttcagc gtgccgtcct
    11701 gcagcacgcg cagcaggcgc acctgcgcgg cgagcggcag ttcgccgatc tcgtccagga
    11761 acagcgtgcc gcgatccgcc tgctcgaacc agccacgccg ggctgcagca gcgccggtga
    11821 aggcgccacg ttcgtgtccg aacagctcgg tatcgatcag atccggcggc agcgccccgc
    11881 agttgacgcg cacgaaggcc tggccggtgc gcggcgagcc ctggtggatc gcacgggcga
    11941 tgacctcctt gcccgagccg gtctcgccga ggatcagcac cggcacatcg gtcgccgcca
    12001 ccaggcgcac gcgctccagc accggccgca gcccgccatc agcgccgacg atggtttccg
    12061 tgctgcgcac cggctcgcgg tcgcgcagct ggctgacgag ttcgtgcagc ccgctcagtt
    12121 cgctgcgcac gtcctgccag tcgagcaagg tgcgcaaacc cgccgcgatg cgcgccagac
    12181 agccccgcag gccgggtccg atcccggctc ccgcccgcag atgcagcagc gccagcgcac
    12241 tgtgttctcc ggcagagccg gccagtccca gtgcagccat cggccccgcg ccggacacga
    12301 gatcggcgcg cgaggccgac agcgtcggca gggccttgag ccagcgcggc cggctgcggc
    12361 cgttggtcgt ttcgatcgtc gaggcggcac accagctcgt cagtgcactg agctcggcag
    12421 ccggcagcgg gcagcgcgag gaacgggttt cgtccgcagc ctcacagcgc cagccacgca
    12481 gctgctgatt gccggcatcg ggaatcagca gctccagcgc ggtgaccggc agggcccggc
    12541 ccaggatgcg cgtgatgtcc ggcagcacgg catccagccg tcccgccgcc gccgggcggg
    12601 cgacgaactg ccagaggtcg agcaaggcca gcaagagggc ttgcgtcggt tcatcggaag
    12661 aatgatgttc gagcatattt gacggatata tcagtaaata agatagatac cagaatatat
    12721 gacggataaa acagcatgaa tattcatatg gaatattttg ttttggaaaa cccatcatcg
    12781 agttattaaa aatcgcgaag tcggcacgcc gtttgctcat cccaccgtac ccgctctttc
    12841 cgcttcgtac ggtgatccat gactctcctg cccttgcgcg ccgccctgtt cggctggctg
    12901 tcggcctgct gcctcgccgg cagcggcaac gtgaatgccg ccgatctcga actgctgaat
    12961 gtttcctacg atccgacgcg cgagctgtat caggcctata acgccgcctt cgtgcgcgag
    13021 tggcaggccg ggcatccggg cgacacgctg acgatccgcc agtcccatgg cggttccggc
    13081 aagcaggcgc gcgccgtcat cgacgggctc gccgccgatg tcgtgacgct ggccctggcc
    13141 ggtgacatcg acgccgtggc cagatcgggc ctgatcgccg gcgactggca gaagcgcctg
    13201 ccgcacgcca gcacgccgta ctactcgacg atcgtgttcc tggtgcgcaa gggcaacccg
    13261 aagggcctga aggactgggg cgatctggcc cggcccgggg tggcggtgat cacgccgaat
    13321 ccgaagacct ccggcggtgc ccgctggaac tacctggcat tgtggggctg ggcactccgg
    13381 cagcccggcg gcagcgagga ctcggcccgg gccctgctcg aaaaggtctt ccgcaacgtg
    13441 cccgtgctcg acaccggcgc ccgcggctcg acgacgacct tcgtgcagcg tggcatcggc
    13501 gacgtgctgc tcgcctggga gaacgaagcc ctgctggcgc tgaaggaata cggcagcgac
    13561 gccttcgaga tcgttgtgcc gaagaccagc atccgggccg agccgccggt ggcggtcgtc
    13621 gacaaggtcg tcgaacgacg cggcacgcag acgctcgccg acgcctacct gcgcgggctc
    13681 tacgagccgg ccgcccagcg catcgcggcc gagcatggct accggcccac cgatgcggcg
    13741 gtcgccgccg aattccgcgc ccgcttcccg cagatcgagc tgttcggcat cgacgacttc
    13801 ggcggctgga gcgccgtgca gagcaagcac ttcgccgatg gcggcgagtt cgaccgtctc
    13861 taccagcccg gcagccgctg ac
//
LOCUS       DAOQIE010000057        13867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2121_length_13867_cov_6.827396, whole genome shotgun sequence.
ACCESSION   DAOQIE010000057 DAOQIE010000000
VERSION     DAOQIE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_13867_cov_6.827396"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK144_05410"
     CDS             <1..865
                     /locus_tag="PK144_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65197.1"
                     /translation="QHDAEPAELEALTAAGVPWWRPEDLDLRNDLDGLAALLTALDLT
                     LGAATATTELAGALGRPAWRLYPYPAWVALGSGGCPWHPQMLEFTRAGGIDAPWEALL
                     AGLGATLDAALAPVASPAAPTLAALLDELLGEGQCAVLAGDVSAEWALPEVMQRLGPD
                     GLLLRFDPDGTGPAAGTLWPGRLAAAEALAVTAATPAAWLAAQRQREQTLDGLKLPHC
                     DWLVSGADTDAAAVLAGATQVIARCRPFLLLGAAAAAPAGYTLMECRDDAGRAWRLAF
                     PLPQSDTNGDA"
     gene            979..3015
                     /locus_tag="PK144_05415"
     CDS             979..3015
                     /locus_tag="PK144_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNF65198.1"
                     /translation="MPQYINSNIPSLNAQRNLNASQNALQTSLQRLSSGMRINSAKDD
                     AAGLAISDRFTSQIKGINQATRNANDGISLAQTAEGALGEITNNLQRLRELAVQSANA
                     TNSDSDRAALQQEAGQLLQEIDRSAKASNFNGTNLLDGSFKGANFQVGANVGETINVS
                     LNGATTDKLGVSNAVSVSSVSTANAIASGDLSINGVLIGPSVASSDHASFSGNAASSI
                     AKAAAINAKTSETGVTATVDKNIAEGASMTGASATGAILINGVTTATFATSTDTALTR
                     KNVVEAINAISARTGVTAVDTGIDTGGVQLVAADGRNITTSFVTVTSAATGVMAAGTN
                     QGTVSLSSDQDIVIAGGTTNALGTNAGFTAGTYKTQEATVSTISNNGTALASGDFSLN
                     GVLVGASRSSDDTASTGTTAQRAGSAIAKVAAINRASEQTGVTATANTTRVNGTTQVG
                     ASTTGRLVINGVTTATFSTTTDTAASRAAAITAINAISGRTGVSAIDDGTNGVRLEAA
                     DGRNIIHSFTGVTAASTGVGAAATTFGTYTLHSASAIDVGAGTTNVVTGVANSGLGVG
                     TYGETKTGSSLKDLDISTVDGANKALTAIDNALKQIDENRSTLGAVQNRFSSTLNTLS
                     TNAENLTAARSRIQDADFASETATLSKNQILQQAGTAMLAQANQLSQGVLSLLR"
     gene            3114..3551
                     /locus_tag="PK144_05420"
     CDS             3114..3551
                     /locus_tag="PK144_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNF65199.1"
                     /translation="MTLSIPPAGGAIERPPATAVAVQQAISPGAAQNLTPEAVAPVDP
                     VAAIAALGDVRGRAGNEPQQQGSVEERERELQRATEDVNRYLSESHRALNFSIDRESG
                     ETVVKVMDTEKNEVIRQIPSEEVLALARRLRDGEEGYLVHAQA"
     gene            3589..5613
                     /gene="fliD"
                     /locus_tag="PK144_05425"
     CDS             3589..5613
                     /gene="fliD"
                     /locus_tag="PK144_05425"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNF65200.1"
                     /translation="MAAISSPGLGSGLDVNNLVSQLMAAERKAPTARLDSASTLNDAR
                     ISALGKITSALDKLQTAAGTLASPNTFQTKTATVSDPSALSVTAARTAAAGSYTVEVS
                     QLARADKLASASFNTAFETVGTGTLTISFGTWNSGSSTFTPNAAKPSTSIVIDATNNT
                     LSGIRDAINAAGAGVTASIVGDGSGQRLVFSGSESGAANGLKISVSDNDGNSTDAGGL
                     SQFAFDPAATAGSGRNLTQTVSAQDAKFKLDGLSLTSSTNLVGSAVSGLTLNLRTVTS
                     SPVTVDIARDDSAAAKAVSGLVDAYNEVVSAVKSASSFDSESKKGGPLIGDASVLSVM
                     GRLRGLMTSSIAGLGGVSSLKAVGVSFQKDGTLSFDSSTLADALSSNADSVARLFGAT
                     GKASDSRVSVVVSSSTTKTGNYAVDITTEPAGGLLTGAVTAPGFPLTIDGTNNGLSLL
                     IDGTATGALTLTQGSYASADDLAHELQSRINGASALKTAGAAVTVSWNTDHFEIRSNR
                     YGAASTVSLASGSAATALGLDTGTATAGTDVAGTIGGVAATGSGRILTASGDADGLRL
                     LISGAGTGARGSVDFKRGLGDQLKSLLDDVLADDGTIGRRSSGLSDESARIAKQRTAL
                     DDRMTELETRYRTQFTRLDSVMASLKATSDFLTSQLNAMTNSGSSSSSSN"
     gene            5717..6106
                     /gene="fliS"
                     /locus_tag="PK144_05430"
     CDS             5717..6106
                     /gene="fliS"
                     /locus_tag="PK144_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNF65201.1"
                     /translation="MTPAQQALAQYNRIGTVSAVEDASPHRLIQLLLDAGLERIAHAR
                     RALEAGDVAARGLAIGKALDIVAGLRGSLNFEAGGEIATNLDALYEYLERRLLQANLE
                     ADAGMLDEAATLLRQVKEGWDGIADAA"
     gene            6103..6414
                     /locus_tag="PK144_05435"
     CDS             6103..6414
                     /locus_tag="PK144_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNF65202.1"
                     /translation="MNPVDWHAQAAGVLAATETMLRHAEAQDWSSLAEAEAGRKPALA
                     RLLPAPAAVDPAAVLAFIEAVLASDQRTARLVGESRDEAGQALRGLMAAGHARRAYGG
                     A"
     gene            6498..6965
                     /locus_tag="PK144_05440"
     CDS             6498..6965
                     /locus_tag="PK144_05440"
                     /inference="COORDINATES: protein motif:HMM:NF028133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNF65203.1"
                     /translation="MARQETEAAREVLRSLLTLALGPGEATDDVAAAGGGERGGELLR
                     LERKLDLVLELLGRLLIRAENLPPLHKVRLSAETVLWQSDEIPSPGPVAVELHLDLRY
                     PLPLCLSGNAQVDGDQVRVDFASTDPTLRDALERLIFLLHRRRLGRERRPSGR"
     gene            7055..8506
                     /locus_tag="PK144_05445"
     CDS             7055..8506
                     /locus_tag="PK144_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF65204.1"
                     /translation="MDKPVLILDDARERARELRGVLEFMEEGVQILQLGRSQLREALG
                     GRPAYEVVFVGQGSNAEADGSLLRELRNALPQVPLVLITEEAGMPGACSLVPGVFTIL
                     RPFRHASLSALQSQLAEYRQAQASGQIGPLNRRHAELFRALVGSSPAIVAVRKLIERV
                     APSDATVLILGESGTGKEVVARNLHYFSARQKGPFVPVNCGAIPENLLESELFGHEKG
                     AFTGAITARRGRFELAEGGTLFLDEIGDMPMPMQVKLLRVLQERTFERVGSNKAIEAD
                     VRIVAATHRKLEEQIASGAFREDLYYRLNVFPIEMPPLRERIGDLPQLIDDLLRRQEN
                     QSGSRMTLTPAALRCLGRYPWPGNVRELANLIERLAILQGGGGAVEVPDLPPKYQIGA
                     ETEIHEPADSHAALAEDAEGAVPPAAGRNLHLPAGGLDLKQYLSDLETQLIVSALEES
                     NGVVAHAASLLKMRRTTLVEKIRKYGIKAADDE"
     gene            8503..9234
                     /locus_tag="PK144_05450"
     CDS             8503..9234
                     /locus_tag="PK144_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNF65205.1"
                     /translation="MSEPVPETFRTAGAAGAAGAAGAALPSVLLFDLDGTLVDSAPSI
                     LESFRLAFAACGIEPAVALDARLIGPPLRPTLTRLLGGEADAPTLDRLASAFRDAYDG
                     GGWRATVAYPGLEAVVGRLADAGRRLFVVTNKRLTPTRLILDHFGLGPRFEAVYALDA
                     FEPPVADKAAALAVVLARHGLAAADCVYIGDTPEDAAAAAANDLAFIAAGWGYGDPAR
                     LAAAAAQARVGTLAELAELLLAPEF"
     gene            9396..10181
                     /locus_tag="PK144_05455"
     CDS             9396..10181
                     /locus_tag="PK144_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65206.1"
                     /translation="MNPTRHPLEADRPVDGQSRIPSPGVPAGDACDEFEQRRSERLQR
                     LRRLGDLMAHLAERIETPDPSMLSAGDALPRAEDELLERLQQLEVTVRNLLICVRGGG
                     LERRPLPAAVLIGRCIDALLPQLGATGATLAVDSTLPAEAEVLVHPEAFVAMLQTLAE
                     PWLAHGVRLQLGFHAGADGRPQLDCRRHAPGAQPARRDPLAARVARIVAQAHAAELSG
                     DPADGRLTLRLPALDLPRDPAAEAGIPAAAAGNGPPAGGQRVA"
     gene            10262..10579
                     /gene="fliE"
                     /locus_tag="PK144_05460"
     CDS             10262..10579
                     /gene="fliE"
                     /locus_tag="PK144_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNF65207.1"
                     /translation="MTLSPLNASIAALRAAATDAGAAAGPEAGAGDADFASLLKGALD
                     RVDARQQQASRMARAFELGEEGADLPDVMIAMQQSRLSFQSALQVRNKLVAAYQDILN
                     MPL"
     gene            10632..12662
                     /gene="fliF"
                     /locus_tag="PK144_05465"
     CDS             10632..12662
                     /gene="fliF"
                     /locus_tag="PK144_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNF65208.1"
                     /translation="MTEDTSLATATDTADAAPARAGWRDWLVRVRTPGPWMYGAIALG
                     VLLLATIVAVALSAGRPDFQPLYHGLAEADAGQIMEALNKMQVPVRVDRQTGTLNVPA
                     DRVAELRLKLAAQGLPRGTTGGFDTFEQQSGFGTSQFMENARYQHALEGELARSIMSI
                     GAVANARVHLALPKDTVFVRNKQQPTASVLVQLQPGRAIDAGQVAAIVHLVSSSVPRL
                     TPSQVSVVDQAGNLLTRPAGGTPGLNLDQMEYQQRLESNYTRRIEDLLTPILGANKMR
                     AQVALDLDFSQLEETSESFDPKRERGMVRSEELIEENNNRPQPMGIPGALSNQPPGVA
                     TAPEQAAGAPAAQADPNNPNAPQPGGGQTGNGQIGPQGQAAAAAGAQQSDAQRAADGS
                     RPVRTRSQSTRNYELDKRVSHITAAPGRVRRVSVAVVLDDRITVRPEGSVERVSWPQE
                     DIDRITALVREAVGFDAQRGDTVNVMNATFATTPFDPNLLPFWRQDWFIELVKWSLVA
                     IVAIVFLLVVVRPVVRRLLPPPPALPAPEPAAAAALAAPAATETGPGTALATTDAGGK
                     ALVPAEGGEASGDEAGAGGDEDADAVQLSAAGQALAQADGHHGNGNGKAPVPLPQPDD
                     AEAIERLEERLDIARRLVAEDPRRAVSVLRTWMNRQPGEGMALLASQEEEEA"
     gene            12690..13679
                     /gene="fliG"
                     /locus_tag="PK144_05470"
     CDS             12690..13679
                     /gene="fliG"
                     /locus_tag="PK144_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNF65209.1"
                     /translation="MSGPDRVAVLMMALGEEYAAELLKQFDPRELHRIGASMASLSSV
                     GRDAVSTVVAEFNNELVNQNALVIDSRDFMKNVLTRALGRDKAKNVMDRILNEAGATG
                     VEALKWMEAQAVAAGLRREHPQIIALVLSYLESAQAAEVLALLPETLRHDVLIRVATL
                     DSVPTAAIGELNEVIEKQVMRNINAASSFKVGGPKRAAEILNAIDTGISTPLMDKIKG
                     MDESLALRIEDLMVVFEHLLNADDRGIQNLLREVSSETLIVAMRGADETIREKILRNM
                     SKRAAEMMRDDLESMGPVRLADVEAAQKDIMTIAKRMAEAGDLNLGSGGGGYV"
     gene            13672..>13867
                     /locus_tag="PK144_05475"
     CDS             13672..>13867
                     /locus_tag="PK144_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65210.1"
                     /translation="MSDRIIPRQAGRDLPRWQFPGFDKAQPEAAPEPVAAPPGKSAGA
                     LKSDRPALRRGSASRFSWVSR"
BASE COUNT         2248 a         4991 c         4727 g         1901 t
ORIGIN      
        1 gcagcacgat gccgagcccg ccgagctgga ggcgctgacg gcggccggcg tgccgtggtg
       61 gcggccggag gatctcgacc tgcgtaacga cctcgatggc ctcgcggcgc tgctgacggc
      121 gctcgatctc accctcgggg cagcaacagc cacgaccgaa ctcgccggtg cactcgggcg
      181 gccggcgtgg cggctgtatc cgtatccggc ctgggtcgcg ctcggcagcg gcggctgtcc
      241 gtggcatccg cagatgctcg aattcaccag ggcgggcggc atcgatgcgc cgtgggaggc
      301 gctgctcgcc gggctcggcg cgacgctcga cgcggcgctg gcgccggtcg cctcgccggc
      361 ggcaccgacg ctcgccgccc tgctcgatga actgctcggc gaaggccagt gcgcggtgct
      421 cgccggtgat gtgtcggccg aatgggcact gcccgaggtg atgcagcgcc tcggacccga
      481 cggcctgctg ctgcgcttcg atccggatgg cacggggccg gcggccggca cgctgtggcc
      541 gggccggctg gcggcggcag aggcgctggc cgtcacggcc gcgacgccgg ccgcgtggct
      601 cgccgcgcag cgccagcgtg agcagacgct cgacggcctg aagctgccgc actgcgactg
      661 gctggtgagc ggtgccgata ccgacgctgc ggcggtgctg gccggtgcga cgcaggtgat
      721 cgcgcgctgc cggccgttcc tgctgctcgg cgcggctgcc gcagcgccgg ccggctacac
      781 gctgatggag tgccgagacg atgccggaag ggcatggcgg ctggcctttc cgctgccaca
      841 gtccgacacg aacggggatg cctaaagatt tcccgagaag cgccgatacc cggaatcaag
      901 gcggcacgga cccttccggg tcactcgccg gaaggcccgt ccgggtcgta gccatcggct
      961 cttctgagga ttgcagtcat gccccagtac atcaacagca acattccctc gctgaacgcc
     1021 cagcgcaacc tgaacgcctc ccagaacgcc ctgcagacct cgctgcagcg cctgtcctcg
     1081 ggcatgcgca tcaacagcgc caaggacgac gccgccggcc ttgcgatttc cgatcgcttc
     1141 acctcgcaga tcaagggcat caaccaggcc acgcgcaacg ccaacgacgg catctcgctg
     1201 gcgcagacgg ccgaaggcgc gcttggtgaa atcaccaaca acctgcagcg cctgcgtgaa
     1261 ctggccgtgc agtcggccaa cgccacgaac agcgattccg accgcgccgc gctgcagcag
     1321 gaagccggcc agctgctgca ggaaatcgac cgctccgcca aggcttccaa cttcaacggc
     1381 accaatctgc tcgatggcag cttcaagggt gcgaatttcc aggtcggcgc caacgtcggc
     1441 gagaccatca acgtctcgct caacggcgcg accactgaca agctcggcgt cagcaacgcc
     1501 gtgtcggtca gctcggtgtc gaccgcgaac gcgatcgcca gcggtgatct gtcgatcaac
     1561 ggcgtgctga tcggtccgtc ggtggcgtcc agcgatcacg cctcgttcag cggcaatgcc
     1621 gccagctcga tcgccaaggc cgcggcgatc aacgccaaga cttccgagac cggcgtcacc
     1681 gccaccgtcg acaagaacat cgctgaaggc gcgagcatga ccggtgccag cgcgaccggt
     1741 gcgatcctca tcaacggcgt gaccacggcg acctttgcga ccagcaccga tacggcgctg
     1801 acgcgcaaga acgtcgtcga ggcgatcaac gccatctcgg cccgcaccgg cgtgaccgcc
     1861 gtcgataccg gcatcgacac cggtggcgtg cagctcgtcg ctgccgacgg ccgcaacatc
     1921 acgacttcct tcgtgaccgt cacctccgcg gccaccggcg tcatggcggc cggcaccaac
     1981 cagggtacgg tgtcgctgtc ctcggaccag gacatcgtca tcgccggtgg cacgaccaac
     2041 gcgctcggca ccaatgccgg tttcacggca ggcacctaca agacccagga agccacggtt
     2101 tcgacgatca gcaacaacgg cacggcgctc gccagtggcg acttcagcct caacggcgtg
     2161 ctggtcggcg cttcccgctc ctcggatgac accgcctcga ccggcacgac cgcccagcgc
     2221 gccggcagcg ccatcgccaa ggttgcggcg atcaaccgcg ccagcgagca gaccggcgtc
     2281 acggccaccg ccaacaccac gcgcgtcaac ggcaccacgc aggtcggcgc ctcgaccacc
     2341 ggtcgtctgg tgatcaacgg cgtgaccacc gcgaccttct cgacgacgac cgacaccgcg
     2401 gcctcgcgcg ctgcggcgat caccgcgatc aacgcgatct cgggccgcac cggtgtctcc
     2461 gcgatcgacg acggcaccaa cggcgtgcgc ctcgaagccg cggatggccg caacatcatc
     2521 cactcgttca cgggcgtgac cgcggccagc accggcgtcg gcgctgcggc gaccaccttc
     2581 ggcacctaca cgctgcactc ggcgagcgcg atcgacgtcg gtgccggcac gaccaacgtc
     2641 gtcaccggtg ttgcgaactc cggcctcggt gtcggcacct acggcgaaac caagaccggc
     2701 agctcgctga aggatctcga catctcgacg gtggacggag ccaacaaggc gctgacggcg
     2761 attgacaacg cgctcaagca gatcgacgag aaccgctcga cgctcggtgc cgtgcagaac
     2821 cgcttcagct cgacgctgaa cacgctctcg accaacgccg agaacctgac cgccgcccgt
     2881 tcgcgcatcc aggatgccga cttcgcctcg gaaacggcca cgctcagcaa gaaccagatc
     2941 ctgcagcagg ccggcaccgc gatgctcgcg caggccaacc agctctcgca gggcgtgctg
     3001 agcctgctcc gctaagctca gaccggctga caggaggcgc atgagcgccc gttgaaaacg
     3061 gcccgggggc ggagagtacg cccccgggcc tttgggcttt cgggaggtgg atcatgacct
     3121 tgagcattcc gccggctggc ggcgcgatcg agcgcccgcc ggcgacggcc gtcgcggtac
     3181 aacaggcgat ctcgccgggc gcggcccaga acctgacgcc ggaggcggtc gcaccggtcg
     3241 atccggtggc ggcgatcgcc gcgctcggtg acgtgcgcgg ccgtgccggc aacgaaccgc
     3301 agcagcaggg cagcgtcgag gagcgcgagc gcgaactgca gcgcgcgacc gaggacgtca
     3361 accgctatct gtccgagtcg caccgcgcgc tgaatttcag catcgacagg gaaagcggcg
     3421 agaccgtcgt caaggtcatg gataccgaga agaacgaggt catccgccag atcccgtccg
     3481 aagaagtcct ggcgctggca cgacgcttgc gtgatgggga ggagggatac ctcgtacacg
     3541 cccaggcctg acggatttcc gtcacctttg cccgagagca caagcgccat ggcagccata
     3601 tcctcccccg gactcggctc cggactcgac gtgaacaacc tcgtctcgca gctgatggcg
     3661 gcggaacgca aggcgccgac ggcgcgcctc gacagcgcca gcacgctgaa cgatgcgcgc
     3721 atctcggcgc tcggcaagat cacgtcggcg ctcgacaagc tgcagaccgc cgccggcacg
     3781 ctggcgagcc cgaacacctt ccagaccaag acggcgaccg tcagcgatcc gtcggcgctg
     3841 tcggtgaccg ccgcgcgcac cgccgccgcc ggcagctaca cggtcgaggt ctcgcagctc
     3901 gcgcgcgccg acaagctcgc aagtgccagc ttcaacacgg cattcgagac cgtcggcacc
     3961 ggcacgctga cgatctcgtt cggcacctgg aactcgggca gcagcacctt cacgccgaac
     4021 gccgccaagc cctcgaccag catcgtcatc gatgccacca acaacacgct ctccggcatc
     4081 cgcgatgcga tcaacgccgc cggtgccggc gtcacggcca gcatcgtcgg cgatggcagc
     4141 ggccagcgcc tggtgttctc gggcagcgag tcgggggccg cgaacgggct gaagatcagc
     4201 gtcagcgaca acgacggcaa ttcgaccgat gccggcgggc tgtcgcagtt cgccttcgat
     4261 ccggccgcga ccgccggcag cggcaggaat ctgacgcaga ccgtcagcgc ccaggacgcc
     4321 aaattcaagc tcgatggcct gtcgctgacc agcagcacga atctggtcgg ctcggcggtg
     4381 tcgggcctga cgctgaacct caggaccgtg accagcagcc cggtcaccgt cgacattgcc
     4441 cgcgacgaca gcgccgcggc caaggcggtg tcgggtctgg tcgatgccta caacgaagtg
     4501 gtgtcggccg tgaagtcggc ctccagcttc gacagtgaat cgaagaaggg cgggccgctg
     4561 atcggcgatg cctcggtgct gtcggtcatg gggcggctgc gcggcctgat gacttcctcg
     4621 atcgccggcc tgggcggtgt gtcgagcctg aaggccgtcg gcgtgtcctt ccagaaggac
     4681 ggcacgctga gctttgattc gagcacgctc gccgatgccc tgagcagcaa tgccgattcc
     4741 gtcgcccgcc tgttcggtgc caccggcaag gccagcgaca gccgcgtgtc ggtggtcgtg
     4801 tcgtcctcga cgaccaaaac cggcaactac gcggtggaca tcactaccga gccggccggc
     4861 ggcctgctga ccggcgcggt cacggcgccg ggtttcccgc tgaccatcga cggcaccaac
     4921 aacggcctga gcctgctcat cgacggcacc gccaccggtg cgctgacgct gacccagggc
     4981 agctatgcga gtgccgatga tctcgcgcac gagctgcaga gccgcatcaa cggtgcctcg
     5041 gcgctgaaga ccgccggtgc cgcggtgacc gtgagctgga acaccgatca cttcgagatc
     5101 cgctcgaacc gctacggcgc ggcctcgacc gtgtcgctcg ccagcggctc ggcggcaacc
     5161 gcgctcggcc tcgataccgg cacggccacg gccggcaccg atgtcgccgg caccatcggc
     5221 ggcgttgccg ccaccggcag cggccgcatc ctgaccgcaa gcggcgatgc cgacgggctg
     5281 cggctgctga tctccggggc cggcaccggc gcgcgcggca gcgtcgactt caagcgcggg
     5341 ctcggggacc agctcaagtc cctgctcgat gacgtgctcg ccgatgacgg cacgatcggg
     5401 cggcgcagca gcggtctcag tgacgagagc gcgcgcatcg cgaagcagcg taccgccctc
     5461 gacgaccgca tgaccgaact cgaaacccgc taccgcacgc agttcacgcg gctcgactcg
     5521 gtgatggcga gcctgaaggc gaccagcgac ttcctgacct cgcaactgaa tgcaatgacc
     5581 aactccggca gcagttccag cagttcgaac tgacggatgc ggtgttcgca ggcgatcaca
     5641 ccggcccggc tgcggccgcg ccaatccacc cacggccccg ccggcacgcg cgggacgtac
     5701 tccagaggaa cccgacatga cccccgccca gcaggcactc gcgcagtaca accgcatcgg
     5761 taccgtcagt gccgtggaag atgcctcgcc gcaccggctg atccagctgc tgctcgacgc
     5821 cggccttgag cgcatcgccc atgcgcgccg ggcgctggaa gccggcgatg tcgctgcgcg
     5881 cggcctggcc atcggcaagg cgctcgacat cgtggccggc ctgcgcggca gcctgaactt
     5941 cgaggccggc ggcgagatcg cgaccaatct cgatgcgctg tacgaatacc tcgaacgccg
     6001 gctgctgcag gccaacctcg aagccgatgc cggcatgctc gacgaggcgg cgacgctgct
     6061 gcgccaggtc aaggaaggct gggacggcat cgccgacgcc gcgtgaaccc cgtggactgg
     6121 cacgcgcagg ccgccggcgt gctcgccgcc accgagacca tgctgcgtca cgccgaggca
     6181 caggactggt cgtcgctggc cgaggccgag gccgggcgca agccggcgct cgcccggctg
     6241 ctgccggcac cggccgcggt cgatccggcc gccgtgctcg ccttcatcga ggccgtgctc
     6301 gccagcgacc agcgcacggc ccggctggtc ggcgagtccc gcgacgaagc cggccaggcg
     6361 ctgcgcggcc tgatggcggc cggtcacgcc agacgcgcct acggcggggc ctgagcatgg
     6421 aagcgggcga ggggctgtat cacgaggcac tgctgccgct ggcgtggcgg gcgctgccgg
     6481 agctgcccgg tcccgaaatg gcccggcagg agaccgaggc cgcgcgcgag gtgctgcgtt
     6541 cgctgctgac gctggcgctc ggccccggcg aggcgaccga tgatgtcgcc gctgccggcg
     6601 gcggcgaacg tggcggcgaa ctgctgcggc tggagcgcaa gctcgatctg gtactcgaac
     6661 tgctcggcag attactgatt cgcgccgaaa atctgccgcc ccttcataag gtgaggctgt
     6721 ccgccgaaac cgtgctgtgg caaagtgacg aaattccgtc gcctggtccg gtcgccgtcg
     6781 aactccacct cgatctgcgc tatccgctgc cgctgtgcct gagcggaaat gcgcaggtcg
     6841 acggcgacca ggtccgcgtt gacttcgcca gcaccgatcc gacgctgcgc gatgcactcg
     6901 aacgcctgat cttcctgctc caccgccgtc ggctcggtcg cgaacggcgt ccttcggggc
     6961 gttgaccggg cgatatcccc aactatggtt gtgggctcgc cggaccagcc ctggcccgcg
     7021 atcccccccg tcgaaacacc gagtccggtc tgcaatggac aagcccgtac tgatcctcga
     7081 cgacgcgcgc gagcgcgccc gcgaactgcg tggcgtgctc gaattcatgg aggaaggcgt
     7141 acagatcctg cagctgggac gcagccagct gcgcgaggca ctcggcggcc ggccggcata
     7201 cgaggtggtc ttcgtcggtc agggcagcaa tgccgaggcc gacggcagcc tgctgcgcga
     7261 gctgcgcaat gccctgccac aggtgccgct ggtgctgatc accgaggaag ccggcatgcc
     7321 cggcgcctgc agtctcgtgc cgggcgtgtt cacgatcctg cggccgttcc ggcatgcctc
     7381 gctgtcggcg ctgcagtcgc agctcgccga ataccgtcag gcgcaggcca gcggccagat
     7441 cgggccgctg aaccgccgcc atgccgagct cttccgcgcg ctggtcgggt cgagcccggc
     7501 catcgtcgcg gtgcgcaagc tgatcgagcg cgtcgcgccc tccgatgcca cggtgctgat
     7561 cctcggcgag tccggcaccg gcaaggaggt cgtcgcgcgc aacctgcatt acttctcggc
     7621 gcgccagaag gggccgttcg tgccggtcaa ctgcggcgcg atccccgaga acctgctgga
     7681 aagcgagctg ttcggccacg agaagggcgc cttcaccggc gcgatcacgg cgcggcgcgg
     7741 gcgtttcgag cttgccgagg gcggcacgct gttcctcgac gagatcggcg acatgccgat
     7801 gccgatgcag gtcaagctgc tgcgcgtgct gcaggagcgc accttcgagc gcgtcggcag
     7861 caacaaggcg atcgaggccg acgtgcgcat cgtcgcggcc acgcaccgca agcttgagga
     7921 gcagatcgcc agcggcgcct tccgcgagga cctgtactac cggctgaacg tcttcccgat
     7981 cgagatgccg ccgctgcgcg agcgcatcgg cgacctgccg cagctgatcg acgacctgct
     8041 gcgccgccag gagaaccagt ccggcagccg catgacgctg acgccggcgg cgctgcgctg
     8101 cctcggccgc tatccgtggc cgggcaacgt ccgcgaactc gcgaacctga tcgaacgcct
     8161 ggcgatcctg cagggcggcg gcggtgccgt cgaagtcccc gacctgccgc cgaagtacca
     8221 gatcggcgcc gagaccgaaa tccacgagcc ggccgacagt catgcggcgc tggccgagga
     8281 tgccgagggc gcggtgcctc cggcagcggg gcgcaacctg cacctgccgg ccggcgggct
     8341 cgatctcaag cagtatctgt ccgatctgga gacgcagctg atcgtcagtg cgctcgagga
     8401 atccaacggc gtcgtcgcgc acgctgccag cctgctgaag atgcggcgca cgacgctggt
     8461 cgagaaaatc cgcaagtacg gcatcaaggc cgccgacgac gaatgagcga acccgttccc
     8521 gaaaccttcc gtactgccgg tgctgccggt gctgccggtg ctgccggtgc tgcactgccg
     8581 tcggtgctgc tgttcgacct cgacggcacg ctggtcgatt cggccccgag catcctcgaa
     8641 agcttccggc tcgcgttcgc ggcctgcggc atcgagccgg ccgtcgccct cgatgcccgg
     8701 ctgatcggac cgccgctgcg cccgacgctg acgcgcctgc tcggcggcga ggccgatgcg
     8761 ccgacgctcg accggctcgc ctcggcattc cgtgatgcct acgacggcgg cggctggcgc
     8821 gcgaccgtcg cctatccggg ccttgaagcc gtggtcggga ggctggccga tgcgggccgg
     8881 cggctgttcg tcgtcaccaa caagcgcctg acgccgaccc ggctgatcct cgatcacttc
     8941 ggtctggggc cgcgcttcga ggccgtgtac gcgctcgatg ccttcgagcc gccggtcgcc
     9001 gacaaggcgg cggcactggc ggtcgtgctg gcccggcacg gactggccgc ggccgattgc
     9061 gtctacatcg gcgacacgcc tgaagacgcc gccgctgctg cggccaacga tctcgccttc
     9121 atcgcggccg gctggggcta tggcgatccg gcccggctgg cggcggcggc ggcgcaggcg
     9181 cgcgtcggga cgctggcgga gcttgcggag ctgctgctcg cgccggagtt ttgactgccg
     9241 gcggtgcccg catcgccgtc gaaaatccgg cgccatcatc ggtttacaat tcaaattact
     9301 gaatagaaag gagattcata gtaggcacag gacttgctga gctaccagcg tggacgggaa
     9361 accgcccgtt tgagtagccg caccgaggtg ccgaaatgaa cccgacccgt catccgctcg
     9421 aagccgaccg tcccgtggac ggccagagcc ggattccgtc gccgggcgtg cccgcagggg
     9481 acgcctgcga cgagttcgag cagcggcgca gcgaacgcct gcagcggctg cgccggctcg
     9541 gcgacctgat ggcccacctg gccgagcgca tcgaaacccc ggacccgtcg atgctgtcgg
     9601 ccggcgacgc gctgccgcgc gccgaggacg aactgctcga acgcctgcag cagctcgaag
     9661 tcaccgtgcg caacctgctg atctgcgtgc gcggcggcgg gctggagcgc cggccgctgc
     9721 cggccgcggt gctgatcggg cgctgcatcg atgcgctgct gccgcagctc ggtgccaccg
     9781 gcgcgacgct cgccgtcgac agcacgctgc cggccgaagc cgaggttctc gtccaccccg
     9841 aagccttcgt cgcgatgctg cagacgctgg ccgagccctg gctcgcgcac ggcgtgcgcc
     9901 tgcagctcgg ctttcacgcc ggtgccgacg gacgcccgca gctcgactgc cggcgccacg
     9961 cgccgggtgc ccagcctgcc cgtcgtgatc cgctcgccgc gcgggtcgcg cgcatcgtgg
    10021 cccaggccca cgccgccgag ctgagcggtg atccggccga tggccggctg acgctgcgtc
    10081 tgccggcgct cgatcttccc cgtgacccgg ccgcggaggc cgggatcccc gccgctgccg
    10141 ccggcaacgg cccgcctgcc ggcggccagc gcgtggcctg aaccggccgc gccccgcccg
    10201 cgtacgcccg acacgacccg gcctgatccg gcctgatacc gaatccagga gaccgaagct
    10261 catgaccctg tccccgctga acgcctcgat cgccgccctg cgcgctgccg ccaccgacgc
    10321 cggagccgct gccggccctg aagccggtgc cggcgatgcc gatttcgcca gcctgctcaa
    10381 gggcgcgctc gatcgcgtcg acgcccgcca gcagcaggcc tcgcgcatgg cgcgggcctt
    10441 cgagctcggc gaggagggtg ccgatctgcc cgacgtgatg atcgcgatgc agcagtcgcg
    10501 gctgagcttc cagtcggcgc tgcaggtgcg caacaagctg gtcgccgcct atcaggacat
    10561 cctgaacatg ccgctctgag ccggcggatt ccctgtcagg agcgccttga ggaataccga
    10621 ggaacaccgt cgtgaccgaa gacacctcgc tcgccactgc cacggacacc gccgatgccg
    10681 cgccggcgcg cgccggctgg cgcgactggc tggtgcgcgt gcgcacgccg gggccgtgga
    10741 tgtacggcgc gatcgcgctc ggcgtgctgc tgctcgcgac catcgtggcc gtggcgctgt
    10801 cggccggccg ccccgatttc cagccgctgt accacggtct ggccgaggcc gacgccggcc
    10861 agatcatgga agccctgaac aagatgcagg tgccggtgcg cgtcgatcgc cagaccggca
    10921 cgctgaacgt gccggccgat cgcgtcgccg agctgcgcct gaagctcgcc gcgcagggcc
    10981 tgccgcgcgg cacgaccggc ggcttcgaca ccttcgagca gcagtcgggc ttcggcacca
    11041 gccagttcat ggagaacgcc cgctaccagc acgcgctcga gggcgagctg gcgcgctcga
    11101 tcatgagcat cggcgccgtc gccaacgccc gcgtgcatct ggcgctgccg aaggacacgg
    11161 tgttcgtgcg caacaagcag cagccgaccg cctcggtgct ggtgcagctg cagccggggc
    11221 gggcgatcga cgccggccag gtcgcggcga tcgtgcacct cgtgtcctcg agcgtgccgc
    11281 ggctgacgcc ctcgcaggtc tcggtcgtcg atcaggccgg caacctgctg acgcgcccgg
    11341 ccggcggcac gccgggcctg aacctcgacc agatggaata ccagcagcgg ctggaatcga
    11401 actacacgcg ccgcatcgag gacctgctga cgccgatcct cggcgccaac aagatgcgcg
    11461 cgcaggtggc gctcgatctg gacttctcgc agctggagga gacctccgaa tccttcgatc
    11521 cgaagcgcga gcgcggcatg gtgcgcagcg aggagctgat cgaggagaac aacaaccgcc
    11581 cgcagccgat gggcattccc ggtgcgctgt cgaaccagcc gcccggggtc gcgaccgcgc
    11641 ccgagcaggc cgccggcgcg ccggccgcgc aggccgatcc gaacaatccg aatgcgccgc
    11701 agcccggtgg cggccagacc ggcaacggcc agatcggccc gcagggccag gccgctgccg
    11761 cggccggtgc ccagcagagc gacgcgcagc gcgccgccga cggcagccgc ccggtgcgca
    11821 cgcgcagcca gagcacgcgc aactacgagc tcgacaagcg cgtcagtcac atcaccgctg
    11881 caccggggcg cgtgcggcgc gtgtcggtcg cggtcgtgct cgatgaccgc atcacggtgc
    11941 ggcccgaagg ctcggtcgag cgggtttcgt ggccgcagga ggacatcgac cgcatcaccg
    12001 cgctcgtgcg cgaagcggtg ggattcgacg cccagcgggg cgacacggtg aacgtcatga
    12061 acgcgacctt cgcgaccact cccttcgatc cgaacctgct gccgttctgg cgtcaggact
    12121 ggttcatcga actcgtcaag tggagcctgg tcgccatcgt cgcgatcgtg ttcctgctcg
    12181 tcgtcgtgcg accggtcgtg cgccgcctgc tgccgccgcc gccggcgctg ccggcaccgg
    12241 aaccggcggc cgccgctgcg ctcgcggctc cggccgccac cgaaaccgga ccgggcacgg
    12301 cgctcgcgac caccgatgcc ggcggcaagg cactggtgcc cgccgaaggc ggcgaggcga
    12361 gcggcgatga ggccggtgcc ggcggcgacg aggatgccga tgccgtgcag ctgtcggccg
    12421 ccggccaggc gctggcgcag gccgacgggc atcacggcaa cggcaacggc aaggcgccgg
    12481 tgccgctgcc gcagcccgat gacgccgagg ccatcgagcg gctcgaagaa cgactcgaca
    12541 tcgcccgccg gctggtggcc gaggacccgc gccgcgccgt cagcgtgctg cgcacgtgga
    12601 tgaaccgtca gcccggcgag ggcatggcac tgctggcgtc gcaggaagag gaggaggcct
    12661 gaaccatgag tgaaatggcc ggtgaagccc tgagcggccc ggaccgcgtc gcggtgctga
    12721 tgatggcgct cggcgaggag tacgccgccg agctgctcaa gcagttcgat ccgcgcgagc
    12781 tgcaccgcat cggcgcctcg atggcgagcc tgtcctcggt cgggcgcgat gcggtgtcga
    12841 cggtcgtcgc cgagttcaac aacgaactcg tcaaccagaa cgccctcgtc atcgacagcc
    12901 gcgacttcat gaagaacgtg ctgacgcgcg ccctcggccg tgacaaggcc aagaacgtca
    12961 tggaccgcat cctcaacgag gccggcgcca ccggcgtcga ggcgctgaag tggatggagg
    13021 cgcaggcggt tgcggccggc ctgcgccgcg agcatccgca gatcatcgcg ctggtgctgt
    13081 cctacctcga atcggcgcag gccgccgagg tgctggcgct gctgcccgag acgctgcgcc
    13141 acgacgtgct gatccgcgtc gcgacgctcg actcggtgcc gaccgcggcg atcggcgagc
    13201 tgaacgaggt catcgagaag caggtgatgc gcaacatcaa cgccgccagc agcttcaagg
    13261 tcggcggtcc gaagcgcgcc gccgaaatcc tcaacgccat cgacaccggc atcagcacgc
    13321 cgctgatgga caagatcaag ggcatggacg agtcgctggc gctgcgcatc gaggatctga
    13381 tggtcgtgtt cgagcacctg ctcaacgccg acgaccgcgg catccagaac ctgctgcgcg
    13441 aggtgtcctc ggaaaccctg atcgtcgcga tgcgcggcgc cgacgagacg atccgcgaga
    13501 agatcctgcg caacatgtcc aagcgcgccg ccgagatgat gcgcgacgac ctcgaatcga
    13561 tggggccggt gcgcctcgcc gatgtcgagg ccgcgcagaa ggacatcatg acgatcgcca
    13621 agcgcatggc cgaagccggc gacctcaacc tcggcagtgg cggtggtggc tatgtctgac
    13681 cggatcattc cccggcaggc cggacgtgac ctgccgcgct ggcagttccc cggcttcgac
    13741 aaggcccagc ccgaggctgc gcccgaaccg gtggccgcac cgcccggcaa gtcggccggc
    13801 gccctcaaga gcgaccgccc tgcgctgcgc cgcggctctg cgagccgctt ttcgtgggtc
    13861 agccgaa
//
LOCUS       DAOQIE010000058        13858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2124_length_13858_cov_7.466058, whole genome shotgun sequence.
ACCESSION   DAOQIE010000058 DAOQIE010000000
VERSION     DAOQIE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_13858_cov_7.466058"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /locus_tag="PK144_05480"
     CDS             <1..2259
                     /locus_tag="PK144_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65211.1"
                     /translation="PGAAPAPAAPATPAAGGGTATTTATAAPPPQSRTRTAVKELQSR
                     NYTFVFERIEAAADTGAGYWLPEDISAGKSLKVPDRLDKKTLETLTGKAGGVLLLPRE
                     WSGTPLPAGTGKWQGSAAKGKGVTVQSYSAPSAGAEGSVVVEVPVKDAAPGSTPPVLT
                     LPVRGHPDLPGLAAARITRKVLKDNKGNGGVSGFRIKTLSPVEFTDLGLSDGWGVEGS
                     GLLTIESLPFLKGAQIGVMLIDGELGFAAIFDAGEFKLGGPLKMTGGNLALAIVPGGP
                     LQASGRIDFALGEYASGSIAAGAGPGGVTLDGSLAFEKGLFSGEGHLRYAAGKWSADG
                     SFTLQRKFPGVDKLSLTLGYDGETQLLRLGGDADLSVPGFRKASCAVAADAAGNIALS
                     GSAELADTLPKIKAGRLTLTLNRPAGGDWSFGGSGELEPDLKGVPASAKLLLSYADGL
                     VTGKLAAAYATKLVGGTLTLNAKAGVGEQPEPLKVWGSGNVDFKAAPWLKATVGLTLG
                     EDGTLTVAGKLGIPAPVELFPKKEYKKQILPLAAPSIPIFGFAVAGVNVGVFLNIDGS
                     LDFIASFGPGQLTEASVGITWSPDDEDATELDGKATFVIPAYAGLKLTVDAGLGVGAA
                     VISAIGGLSVWGELGVKASAGLGVDLKWKPGLGIKLNGEAKADAEAVLKLGAGGFIKV
                     VAKIIGTIYEERFPAGEFTLGSGLKIGVTLPVSYEPDKSFELDFSKIDIRTPEISAGD
                     LVDKIKDQLPSP"
     gene            complement(2338..3366)
                     /locus_tag="PK144_05485"
     CDS             complement(2338..3366)
                     /locus_tag="PK144_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF65212.1"
                     /translation="MVQLEVVDSSTPRVQPEPPTISVVLPAHNEGEGIRHAIEVVAGV
                     LRQCNVSHELIVVDDGSRDDTWEQIREAAARHGYVRGLRFSRNFGKEAALLAGLRAAR
                     GKAVITMDADLQHPPSAIPEMLKCWREGAMVVHAVKRDRSTDSFMQRARAKLFNGLIS
                     RLGRIDLESASDYKLLDRTAVEVLVERLPERERFFRGLAQWIGFPQASVPFSVAPRAA
                     GTGKWSLLGLVNLATTAVLSFTGAPLRLVTILGSVTLLFAAIVGGEALVSWVRGNAAA
                     GFLTLEFTLLIIGSFIMISLGIVGEYIAKIYEELKGRPSFLVHERAGFEDEAAETGRE
                     REPSRFAG"
     gene            complement(3375..4196)
                     /locus_tag="PK144_05490"
     CDS             complement(3375..4196)
                     /locus_tag="PK144_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNF65213.1"
                     /translation="MSAGIALVVNADDYGYFDCVSRGILAAHAAGSVGATGVMANGPR
                     FETTAARLRATPALDAGVHLTLSWGEPLTRALAARLAEGRLPGKFAALTAILTGRWPL
                     AEVEAEWDAQIARVADAGLNPVFINSHEHVHMLPALYRVAARLARRHGIRWLRRTTPE
                     WQGAQGLIRNLLLGAMAAATRADSPQPRLLGLAGSGRLGLPELRRALSGLAPGGRYEL
                     MCHPGEFDAAEVRDGRLLAYHDWSRELAALTDPGFRDHCAQAGIRLVSFRALAAE"
     gene            complement(4407..5159)
                     /gene="moeB"
                     /locus_tag="PK144_05495"
     CDS             complement(4407..5159)
                     /gene="moeB"
                     /locus_tag="PK144_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNF65214.1"
                     /translation="MNDEELLRYSRQIMLPEIDIEGQERLRAATALVIGLGGLGSPVA
                     MYLGAAGVGRLLLVDFDHVDTSNLQRQIAHAEADIGRPKVESARDRVRALNPLVAIET
                     IAEALDDEALAAAVARADVVLDCTDNLTTRLAINAACVAAGKPLVTGAAIRMEGQAMV
                     VDPRVPEAPCYRCLYRSAEEPAESCTQSGVLAPVVGIIGSLQAVEAIKVLTGAGEPLT
                     GRLLLMDARTMEFHEVRVPKYAGCPVCGTQSG"
     gene            5330..8080
                     /locus_tag="PK144_05500"
     CDS             5330..8080
                     /locus_tag="PK144_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65215.1"
                     /translation="MTEGSRETAAAADDLAYPAKLAEREKKLLERRRSRAGVPDDAVR
                     LGMAFSGGGIRSATFCLGFTQALARARELRQVDYLSTASGGGYFGGFLGRLYARDYVA
                     GPDKVEAMLDPELPPSAAGVGRDDPAFRPNAFRWLRENGRYLAPNGAGDLLLAFAVAL
                     RNWAAMHLLLASGVLALLLLLQVPILWLPADGAVAAALPGSERLLWWSPLAALLPWVF
                     NFVLLPLGWAYWLIAAREGLDGKAVPALIDWRQPAVAGLLVLLCLFSGHAGAGIDLLL
                     VTGLALLLTRQATRACRSPIPRERADKARDLLSRRLGKALLGFGLLLALVLIDTLGRT
                     WYALLTHTDSGLGAWFIAIGVAILAVVTRASALVELLSKLTGHDAGSGARVSVQMLTL
                     IAALVLGTGALSLIDLAAQAFVWGLEAPAGVPATLVGSGAGGRLAGSEAHTGILLLGL
                     IGSTAVSWAIGSRDSLGFLNRTSLDPLYSARLTRAYLGASNPRRHDDSAFEAVTEVAP
                     GDDIGLDDYHRRGIHRGAPLHLINVTINETVDGKSQIQQQDRHGLTLALGPAGLSAGV
                     RHHAVFVPDGDDDRDDFEETAAIPRDRPQALVFGPTVHDDEDGERLPVFAWEQPANGI
                     APVCAGARLYTAEPLTLGHWLGISGAAFSTGRGQGTSFGVSLLSGFFNARLGRWWDSG
                     VRRQNTWRDLPSRLFGAHALLLREFLARFPGTATRHWFVSDGGHFENLALYELIRRRL
                     PWILAIDAGCDRDFTFDDLGNFVRKARLDFGAEVRFLDADQIRDTELARYGIGALESL
                     RIDRSTADASARACAALASIRYADKSCGVLLYVKATLYADLPADVREYAAGHPAFPHE
                     PTSDQFFDEAQWESYRRLGEHLGRVLLKLPPAGSPVDSANTSPSPGLRQLFADLQQTV
                     GR"
     gene            8319..10379
                     /locus_tag="PK144_05505"
     CDS             8319..10379
                     /locus_tag="PK144_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65216.1"
                     /translation="MDEAKAAGKKPSDLPELAIDLFKDMDNGIALSPDEIKGRNTWMI
                     WTAGNQAFWNHIAQHSFGTIDLLKILDSRKRSERFNYYGLMNEPGFKQASKPDAFGLW
                     LDVPSEPEAAGVDPAIYGRSSGIVGLRLFPNPEFTPEAAKRWDAERYYGDRSYYYDPK
                     LIRPYRVGMSCAFCHVSFHPLHPPADPAAPTWKNLSTNIGAQYFWVGRIFGYDLENDD
                     FIKHLLNSAEPGALDTSFIASDNINNPRTMNAIYNVAARLETGHEEQLTGGALDFPGN
                     TAKMKVPHILKDGADSIGVLGALARVYLNIGEFHQEWLTHFSPMVGFKPQTPIEVKVM
                     QANSTYWLAGQERVGNLAKFFVKAALPTNLADAPGGQDFLPKDAGVVARGKVVFADNC
                     ASCHSSKQPPKEMTRGTPEYQAWMRTEVAKPDFLQNNYLSNEARIPVTVVRTNACAAL
                     ATNAVRGHIWDNFSSETYKTLPSVGDIDVINPVTREPWKFQAPAGGPGYYRVPSLVSV
                     WASAPFLHNNALGIYTNDPSTAGRMKAFDDAAEKLLWPQKRENRIYRTSGVTFFKLNE
                     VFLPDLLKPLADDDGYLRIGPIPAGTPINLLANLDVTVDLKDPKHVGRLLKLLTKIKF
                     DLARIKLGGLSETESTALLKELVPDLLKLSNCPDFVADRGHEFGAELPDADKRALIEF
                     MKLM"
     gene            10454..12286
                     /locus_tag="PK144_05510"
     CDS             10454..12286
                     /locus_tag="PK144_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006530653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNF65217.1"
                     /translation="MSTPEYDYIIVGSGAGGGPLAANLAKAGHRVLLMEAGGDDETDN
                     YRVPAFNGQASEDPLYRWDFFVKHYSERPQRDSKYVAAEDGILYPRCGTLGGCTAHNA
                     MITVLPHDSDWNHIAEITGDATWLSGYMYRYWQRLEACRYVSRPAEGEDNPSGHGYDG
                     WLSTEMADPALAITDPQLTTVLVDAVLEVKHNRIERIDQLLGLRFDPNDRRVQTKSPE
                     GLALTPIATHKGQRAGARDAIRSAQQSHPENLTVQLHSLVTRVLLDEDLRATGVEYLE
                     GASLYRADPRAATQGPEPGARRQVLARREVILCGGAFNTPQLLKLSGIGPRAELEKFG
                     IAVRVELPGVGENLQDRYEVGVASEMRRDFRILNGASFRATGPDGQPDPAYVEWQQGK
                     GLYVANGAVIGIIKRSTPDRPEPDLYIFGLPSFFRGYYPGYSEVIGPVKNHFTWAILK
                     AHTSNCAGSVTLKSADPRDRPDINFRYFDEGNDAGQQDIDAVVAGVEFVREMNKGLLN
                     KLVTRREVVPGPDIESKEQIAQFIKDEAWGHHASCTCRIGPSSDPMAVLDSRFRVYGT
                     KGLRVVDASVFPKIPGFFIVTPVYMISEKASDVILEDAEGIERI"
     gene            12586..13275
                     /locus_tag="PK144_05515"
     CDS             12586..13275
                     /locus_tag="PK144_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65218.1"
                     /translation="MDILREERIKNYLYSTDKTTTTIPLPNITTPIIENKSMPSESFF
                     PSISTLLDELGAEQIRFSFGILQQILQDGPIVEQLSGPTKIICLLPLSHPIALDSFQP
                     LKNKMTFNNSTLARNTTSDRLNISLPSIAFKTGTGRKDHTVELQRTKGEIKESFFCGH
                     PSPREIFKITVARTALSKIDTKRIKISGSRHTAVTLHIEQLKGGGRNTAPEQLEHVTR
                     QSLCKFIFLED"
     gene            complement(13625..>13858)
                     /locus_tag="PK144_05520"
     CDS             complement(13625..>13858)
                     /locus_tag="PK144_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HNF65219.1"
                     /translation="MVQRPLWASTGTKNPALKPVLYVEELAGKETVNTLPPATLDALL
                     KEAEISDKLHHGLPEAKQIIKSLGDLEKISNHR"
BASE COUNT         2583 a         4656 c         4378 g         2241 t
ORIGIN      
        1 cctggcgctg cccccgcccc cgccgcccct gcgaccccgg cggccggcgg cggcaccgca
       61 acgacgacgg ccacagcggc cccgccgccg cagagccgca cgcgcaccgc ggtcaaggaa
      121 ctgcagtcgc gcaactacac gttcgtgttc gaacgcatcg aggccgcggc cgacaccggc
      181 gccggctact ggctgcccga ggacatctcg gccggcaaga gcctgaaggt cccggaccgg
      241 ctcgacaaga agaccctcga aaccctgacc ggcaaggccg gcggcgtgct gctgctgccg
      301 cgtgaatggt ccggcacgcc gctgccggcc ggcaccggca agtggcaggg ctcggccgcg
      361 aagggcaagg gcgtgaccgt gcagagctac tcggccccga gtgccggcgc cgagggttcg
      421 gtggtcgtcg aggtgccggt gaaagacgca gcccccggca gcacgccgcc ggtgctgacg
      481 ctgccggtgc gcggccaccc ggacctgccg gggcttgcgg ccgcgcgcat cacgcgcaag
      541 gtgctgaagg acaacaaggg caacggcggc gtcagcggct tccgcatcaa gacgctgagc
      601 ccggtcgagt tcaccgatct cggcctgagc gacggctggg gcgtcgaggg cagcggcctg
      661 ctgaccatcg aaagcctgcc gttcctgaag ggcgcgcaga tcggcgtgat gctgatcgac
      721 ggcgaactcg gcttcgcggc gatcttcgac gccggcgagt tcaagctcgg cgggccgctg
      781 aagatgaccg gcggcaacct ggcgctggcg atcgtgcccg gcgggcccct gcaggccagc
      841 ggccgcatcg acttcgcgct cggcgagtac gcgagcggct cgatcgccgc cggcgccggc
      901 cccggcggcg tcacgctcga cggctcgctc gccttcgaga aggggctgtt cagcggcgag
      961 ggccatctgc gctacgccgc cggcaagtgg tcggccgacg gcagcttcac gctgcagcgc
     1021 aagttccccg gcgtcgacaa gctgtcgctg acgctcggct acgacggcga aacgcagctg
     1081 ctgcgcctcg gcggcgatgc ggatctcagc gtgcccggct tcaggaaggc gagctgcgcg
     1141 gtcgctgccg atgccgccgg caacatcgcg ctgtcgggca gcgccgagct tgccgacacg
     1201 ctgccgaaga tcaaggccgg gcggctgacg ctgacgctga accgcccggc cggtggcgac
     1261 tggagcttcg gcggcagcgg cgaactcgaa cccgacctca agggcgtgcc ggcctcggcg
     1321 aagctgctgc tcagttacgc cgacggcctc gtcaccggca agctcgcggc ggcgtatgcc
     1381 acgaagctcg tcggcggcac gctgaccctg aacgccaagg ccggcgtcgg cgagcagccc
     1441 gagccgctga aggtctgggg cagcggcaac gtcgacttca aggccgcgcc gtggctgaag
     1501 gcgacggtgg gcctcacgct cggcgaggac ggcacgctga ccgtcgccgg caagctcggc
     1561 atcccggcac cggtcgagct gttcccgaag aaggaataca agaagcagat cctgccgctc
     1621 gcggcgccct cgatcccgat cttcggcttc gcggtcgccg gcgtgaacgt cggggtgttc
     1681 ctgaacatcg acggctcgct cgacttcatc gccagtttcg gtcccggcca gctcaccgag
     1741 gcctcggtcg gcatcacctg gagcccggac gacgaggacg ccaccgaact cgacggcaag
     1801 gccaccttcg tgatccccgc ctacgccggg ctgaagctga ccgtcgatgc cggactcggg
     1861 gtcggcgcag cggtgatcag cgccatcggc ggcctgagcg tctggggcga actgggcgtt
     1921 aaggccagcg ccgggctcgg cgtcgacctg aagtggaagc ccggcctcgg catcaagctc
     1981 aacggcgagg ccaaggccga tgccgaggcg gtgctgaagc tcggcgccgg cggcttcatc
     2041 aaggtcgtcg ccaagatcat cggcacgatc tacgaggagc gcttcccggc cggcgaattc
     2101 acgctcggct cgggcctgaa gatcggcgtg acgctgccgg tcagctacga gccggacaag
     2161 tccttcgagc tcgacttctc gaagatcgac atccgcacgc ccgagatcag cgccggcgat
     2221 ctcgtcgaca agatcaagga ccagctgccg agcccgtgag gcaggaacga cgacggcgcc
     2281 gccggccggg ccggatgcgc cgtcggggac aggcggggga tgccgtctgc gcgcggatca
     2341 gccggcgaag cgcgagggct cgcgctcgcg accggtctcg gcggcttcat cctcgaaacc
     2401 ggctcgctca tggaccagga aggacggccg ccccttcagc tcctcgtaga tcttggcgat
     2461 gtactcgccg acgatgccga ggctgatcat gatgaagctg ccgatgatca gcagcgtgaa
     2521 ctcgagcgtc aggaagccgg ccgcggcgtt gccgcgcacc cacgacacca gcgcctcgcc
     2581 gccgacgatg gccgcgaaca gcagcgtcac cgagccgagg atggtcacca ggcgcagcgg
     2641 cgcgccggtg aacgacagca cggcggtggt ggcgaggttg acgaggccga gcagcgacca
     2701 cttgccggtg ccggcggcgc gcggcgcgac gctgaacggc acgctggcct gcgggaagcc
     2761 gatccactgc gccaggccgc ggaagaagcg ctcgcgctcg ggcaggcgct cgaccagcac
     2821 ctcgaccgcg gtgcgatcga gcagcttgta gtccgacgcg ctttcgagat cgatgcgccc
     2881 gagccggctg atcaggccgt tgaagagctt tgcgcgcgca cgctgcatga agctgtcggt
     2941 cgagcggtcg cgcttgaccg cgtgcacgac catcgcgccc tcgcgccagc acttcagcat
     3001 ctccgggatc gccgacggcg ggtgctgcag gtcggcatcc atcgtgatca cggccttgcc
     3061 gcgcgccgca cgcaggccgg cgagcagcgc ggcctccttg ccgaagttgc gcgagaagcg
     3121 caggccgcgc acgtagccgt gccgggcggc ggcttcgcgg atctgctccc aggtgtcgtc
     3181 gcgcgaaccg tcgtcgacga cgatcagctc gtggctgacg ttgcactgac gcagcacgcc
     3241 ggcgacgacc tcgatggcgt ggcggatgcc ctcgccctcg ttgtgcgccg gcagcacgac
     3301 gctgatggtc ggcggctccg gctgcacgcg cggggtggac gaatcgacga cttcaagctg
     3361 gaccatgatg acgatcactc cgcggcgagg gcccggaagg aaaccagacg gatgccggcc
     3421 tgcgcgcagt gatcgcggaa accggggtcg gtcagcgcgg cgagctcgcg cgaccagtcg
     3481 tgataggcga gcagccgccc gtcgcgcacc tcggcggcat cgaactcgcc ggggtggcac
     3541 atcagttcgt atcgaccgcc gggcgccaga cctgacagcg cccggcgcag ctccggcaga
     3601 ccgagccgac cgctgccggc aagaccgagc aggcgcggct gcggcgagtc ggcacgcgtg
     3661 gcggcggcca tcgcgccgag cagcaggttg cggatcagcc cctgcgcgcc ctgccactcg
     3721 ggcgtcgtgc gccgcagcca gcggatgccg tggcggcgcg cgagccgggc cgcgacccgg
     3781 tacagggccg gcagcatatg gacgtgctcg tggctgttga tgaagaccgg attaagcccg
     3841 gcatcggcga cgcgggcgat ctgcgcatcc cactcggcct cgacttcggc gagcggccag
     3901 cggccggtca agatcgccgt gagagctgcg aacttgccgg gcaggcgacc ctcggcgaga
     3961 cgcgcggcca gcgcgcgggt cagcggttca ccccaactca gcgtcagatg cacgccggca
     4021 tcgagcgccg gcgtggcgcg caggcgcgcg gccgtggttt cgaagcgcgg accgttggcc
     4081 atgacgccgg tggcgccgac gctgccggcg gcgtgcgcgg cgaggatgcc gcggctgacg
     4141 cagtcgaaat agccgtagtc gtcggcgttg acgaccagcg cgatgccggc actcacgcgt
     4201 ggaccgtccg tgccgcgatc cgggcggcag cgggaacggt ggcagcgggc gcgggcaggg
     4261 cttcggtcat ggcgacggcg gaccggcagt ggcgtgcgca ggggcacgac gggcgcgcat
     4321 catgcccgac gcaaatgtcc cgcacaatga ccagaagctg ccgaaaacaa aagaattttc
     4381 ggatttccgc tgcgaggatt ccggcctcag ccggactgcg tgccgcagac cggacagccg
     4441 gcgtatttcg gcacccgcac ctcgtggaat tccatggtgc gtgcatccat cagcagcagc
     4501 cggccggtca gcggctcgcc ggccccggtc agcaccttga tcgcctcgac cgcctgcagg
     4561 ctgccgatga tgccgaccac cggcgccagc acgccggact gcgtgcagct ctcggccggc
     4621 tcctcggccg agcggtacag gcagcggtag cacggcgcct ccggcacgcg cggatcgacg
     4681 accatcgcct gcccttccat gcggatcgcc gcaccggtca ccagtggttt gccggccgcc
     4741 acacaggcgg cgttgatcgc caggcgcgtc gtcaggttgt cggtgcagtc gagcacgaca
     4801 tcggcgcgcg ccaccgccgc ggccagcgcc tcgtcatcga gcgcctcggc gatggtctcg
     4861 atcgcgacca gcggattcag cgcccgcacg cggtcgcggg ccgattcgac cttgggccgg
     4921 ccgatgtcgg cctcggcgtg tgcgatctgg cgctgcaggt tgctggtgtc gacgtgatcg
     4981 aaatccacca gcagcagccg cccgaccccg gccgcgccga gatacatcgc cacgggtgag
     5041 ccgaggccgc cgaggccgat caccagcgcg gtcgccgcgc gcaggcgctc ctgaccctcg
     5101 atgtcgatct cgggcagcat gatctggcgg ctgtagcgca gcagttcttc gtcgttcatc
     5161 gcagggactc cggcgaccgg ccgcagccgg cggggttcaa ttctgaaaaa aagttaaggg
     5221 cagccggcaa gcccggggct ttactgcccc tgagacaccc ggcttcagca cgcgcggccc
     5281 cagcacacac ggacggccgc cggcccggat cgccaccagg ggctatgcca tgaccgaagg
     5341 atcccgcgaa accgctgcgg ctgccgacga cctcgcctat ccggcaaaac tggccgagcg
     5401 cgagaagaaa ctgctcgaac gccgccgctc acgggccggc gtaccggacg atgcagtgcg
     5461 actcggtatg gcattctccg gcggcggcat ccgcagcgcg accttctgcc tcggcttcac
     5521 gcaggccctc gcccgcgccc gcgagttgcg ccaggtcgat tatctgtcga ccgcctcggg
     5581 cggtggctac ttcggcggct tcctcggccg gctgtatgcg cgcgactacg tggccggtcc
     5641 cgacaaggtc gaggccatgc tcgacccgga actgccgccc tccgccgccg gggtcggtcg
     5701 cgatgatccg gccttccgtc cgaacgcctt ccgctggctg cgcgagaacg gccgctacct
     5761 ggcaccgaac ggtgccggtg acctgctgct cgcgttcgcc gtggcgctgc gcaactgggc
     5821 ggcgatgcac ctgctgcttg caagcggcgt gctggcgctg ctgctgctgc tgcaggtgcc
     5881 gatcctgtgg ctgccggccg atggtgccgt ggctgccgcc ctgcccggca gcgagcgcct
     5941 gctctggtgg agtccgctcg cggcgctgct gccctgggtg ttcaacttcg tgctgctgcc
     6001 gctcggctgg gcgtactggc tgatcgccgc gcgcgaaggc ctggacggca aggcggtacc
     6061 ggcactgatc gactggcgcc agccggcggt ggccgggctg ctggtactgc tctgcctgtt
     6121 ttccggtcat gccggggccg gcatcgacct gctgctggtc acgggtcttg cgctgctgct
     6181 cacccggcaa gcgacccgcg cctgccgcag cccgatcccg cgcgagcgcg ccgacaaggc
     6241 ccgcgacctg ctgtcgcgcc ggctcggcaa ggccttgctc ggcttcggcc tgctgctggc
     6301 actggtgctg atcgacacgc tcggacgcac ctggtatgcg ctgctgacgc acacggattc
     6361 ggggctcggt gcctggttca tcgccatcgg cgtcgcgatc ctggccgtgg tcacgcgcgc
     6421 ctcggcgctg gtcgaactgc tgtcgaaact caccggtcac gatgccggca gtggcgcccg
     6481 cgtgtcggtg cagatgctga cgctgatcgc agcgctggtg ctcggtaccg gtgcgctgtc
     6541 gctgatcgat ctggccgcgc aggccttcgt ctggggcctc gaagcgccgg ctggcgtgcc
     6601 ggccacgctg gtcggcagcg gcgccggcgg ccggctggcc ggcagcgagg cacacaccgg
     6661 catcctgctg ctcggcctga tcggcagcac ggcggtcagc tgggccatcg gcagtcgcga
     6721 cagcctcggc ttcctgaacc gcacctcgct cgatccgctg tacagcgcac gcctgacgcg
     6781 cgcctatctc ggcgcctcca atccgcgccg ccacgatgac agcgccttcg aggccgtcac
     6841 cgaagtagcc cccggcgacg acatcggcct cgacgattac caccgccgcg gcattcaccg
     6901 cggcgcaccg ctgcacctga tcaacgtcac gatcaacgag accgtcgacg gaaaatccca
     6961 gatccagcag caggaccgcc acggcctgac gctggcgctc ggtccggccg gactgtcggc
     7021 cggcgtgcgc catcacgccg tgttcgtgcc ggacggcgac gatgatcgcg acgacttcga
     7081 ggaaacagcg gcgatcccgc gtgaccggcc gcaggcgctg gtgttcggcc ccaccgtcca
     7141 cgacgacgag gacggcgagc gcctgccggt gttcgcgtgg gagcagccgg caaacggcat
     7201 cgccccggtc tgcgcaggcg cacgcctgta taccgccgag ccgctgacgc tcggccactg
     7261 gctcggcatc tccggcgcgg cattctcgac cggccgcggt cagggcacga gtttcggcgt
     7321 cagcctgctg tcgggcttct tcaacgcccg actcggacgc tggtgggatt cgggcgtgcg
     7381 gcgccagaac acctggcgcg atctgccctc gcggctgttc ggcgcacacg cgctgctgct
     7441 gcgcgaattc ctggcccgct tcccgggcac ggcgacccgg cactggttcg tgtcggacgg
     7501 cggccatttc gagaacctcg cgctgtacga actgatccgc cggcgcctgc cgtggattct
     7561 ggcgatcgac gccggctgcg atcgcgactt cacgttcgat gatctcggca acttcgtgcg
     7621 caaggcccga ctcgacttcg gcgccgaggt acgctttctc gatgccgatc agatcaggga
     7681 cacggaactg gcacggtacg gcatcggtgc actggaatcc ctgcgcatcg atcgcagcac
     7741 tgcggatgct tcggcacggg catgcgcggc attggccagt atcagatatg ccgacaaaag
     7801 ctgcggcgta ctgttgtacg tgaaggcaac cctctacgcc gatctgccgg ccgacgtgcg
     7861 cgaatacgcc gccggccacc cggcattccc gcacgaaccg acctcggacc agttcttcga
     7921 cgaggcgcag tgggaaagtt atcgtcggct cggcgagcac ctcggccgtg tactgctcaa
     7981 gctcccgccg gccggctcac ccgtggattc cgcaaacaca tccccctcac caggcttgcg
     8041 gcagctgttt gctgatctgc agcaaaccgt cggccgctga tcgatcatca ctggcatcgg
     8101 acatccccag cattgccatc cggacaggat gtctgaatta cgaccaccgc cggataggca
     8161 cgacccccca tccacggcag cgttagccgc gcatccgggc ccagccccgg gaaagcccac
     8221 cacagagagg gacaagagca atgagaagcc tctgcctgcc tgccgccgcc ctggccggcc
     8281 tgttcgccct gcaggtcccc gccttcgcag ccccgccgat ggacgaagcc aaggccgccg
     8341 gcaagaaacc gtctgatctg cccgaactcg cgatcgacct gttcaaggac atggacaacg
     8401 gcatcgcgct gtcacccgac gagatcaagg gccgcaacac ctggatgatc tggaccgccg
     8461 gcaatcaggc gttctggaat cacatcgcgc agcacagctt cggcacgatc gacctgctga
     8521 agattctcga ttcgcgcaag cgctccgagc gcttcaatta ctacggactg atgaacgaac
     8581 ccggattcaa gcaggcaagc aaacccgatg ccttcgggct gtggctcgac gtgccgtccg
     8641 aaccggaggc cgccggcgtc gatccggcga tctacgggcg ctccagcggc atcgtcggcc
     8701 tgcgcctgtt cccgaatccg gaattcacgc ccgaagccgc gaaacgctgg gatgccgaac
     8761 gttattacgg cgaccgcagc tattactacg atccgaagct gatccgtccg taccgggtcg
     8821 gcatgagctg cgcgttctgc catgtgtcct tccatccgct gcatccgccg gcggacccgg
     8881 ccgcaccgac ctggaagaac ctgtcgacca acatcggcgc gcagtatttc tgggtcggtc
     8941 gcatcttcgg ctatgacctc gaaaacgacg acttcatcaa gcatctgctg aactccgccg
     9001 agcccggcgc gctcgatacg tccttcatcg cctcggacaa catcaacaat ccgcgcacga
     9061 tgaacgcgat ctacaacgtc gcggcgcgcc tcgaaaccgg tcatgaggaa cagctcaccg
     9121 gcggtgcact ggatttcccc ggcaacaccg cgaagatgaa ggtgccgcac atcctgaagg
     9181 acggtgccga ttcgatcggc gtgctcggcg cgctggcgcg cgtctacctg aacatcggcg
     9241 aatttcatca ggaatggctg acgcacttca gcccgatggt cggcttcaag ccgcagacgc
     9301 cgatcgaggt caaggtcatg caggccaact cgacctactg gctcgccggt caggagcgcg
     9361 tcggcaacct cgcgaagttc ttcgtcaagg cggcgctgcc gaccaatctc gccgatgccc
     9421 ccggcggtca ggacttcctg cccaaggatg ccggcgtcgt cgcccgcggc aaggtggtgt
     9481 ttgccgacaa ctgcgcgagc tgccattcga gcaagcagcc gccgaaggaa atgacccgcg
     9541 gcacgccgga ataccaggcc tggatgcgca ccgaggtcgc gaagccggac ttcctgcaaa
     9601 acaattatct gtccaatgaa gctcgcattc cggtcacggt cgtcaggacc aatgcctgcg
     9661 cggcgctcgc gaccaatgcc gtgcgcggtc acatctggga caacttctcg tcggaaacct
     9721 acaagacgct gccgtcggtc ggtgacatcg acgtgatcaa tccggtcacg cgcgagccgt
     9781 ggaaattcca ggcgccggcc ggtggccccg gctattaccg cgtgccgtcg ctggtcagcg
     9841 tctgggcctc ggcgccgttc ctgcacaaca atgccctcgg catctacacg aatgacccgt
     9901 ccacggccgg acgcatgaaa gccttcgacg acgccgccga gaaactgctg tggccgcaga
     9961 agcgcgagaa caggatctac cgcacgagcg gcgtcacgtt cttcaagctc aacgaggtct
    10021 tcctgcccga tctgctgaaa ccgctggccg atgacgacgg ctatctgcgc atcggcccga
    10081 ttccggccgg cacgccgatc aacctgctcg ccaacctcga cgtgaccgtc gacctgaagg
    10141 atccgaagca cgtcggccgg ctgctgaaac tgctgaccaa gatcaagttc gatctggcgc
    10201 gcatcaagct cggtggcctg agcgaaaccg aaagcacggc cctgctgaag gaactcgtgc
    10261 ccgatctgct gaagctcagc aactgcccgg atttcgtcgc cgatcgcggc catgaattcg
    10321 gtgccgagct gcccgatgcc gacaagcgtg cactgatcga attcatgaag ctgatgtgac
    10381 cttgcctgcg cccgtccgcc gggcggcgga cgcggcgcgt tcgaacgctg atcccgcttc
    10441 aggagaatcg gccatgagca ccccggaata cgattacatc atcgtcggct ccggcgccgg
    10501 cggcggcccg cttgccgcga atctcgcgaa agccggtcat cgcgtgctgc tgatggaagc
    10561 cggcggcgac gatgaaaccg acaattaccg cgtgccggcc ttcaacggtc aggcctccga
    10621 agacccgctg tatcgctggg atttcttcgt caagcattat tccgagcgcc cgcagcgcga
    10681 cagcaaatac gtggccgccg aggacggcat cctgtatccg cgctgcggca cgctcggcgg
    10741 ctgcaccgcg cacaacgcga tgatcacggt gctgccgcat gacagcgact ggaaccacat
    10801 cgccgagatc accggcgatg cgacctggct gtcgggctac atgtaccgct actggcagcg
    10861 cctcgaagcc tgtcgttatg tctccagacc cgccgagggc gaggacaatc cgagcggcca
    10921 cggttacgac ggctggctca gcaccgagat ggccgatccg gcgctggcga tcaccgatcc
    10981 gcagctgacg acggtgctcg tcgatgcggt gctggaggtc aagcacaacc ggatcgagcg
    11041 catcgaccag ctgctcggcc tgcgcttcga tccgaacgac cggcgcgtgc agaccaagag
    11101 ccccgagggc ctggccctga cgccgatcgc gacgcacaag ggccagcggg ccggcgcgcg
    11161 cgacgcgatc cgttcggcgc agcagtcaca ccccgaaaac ctgaccgtgc agctgcacag
    11221 cctcgtgacg cgcgtgctgc tcgacgaaga cctgcgcgcc accggcgtcg aatacctcga
    11281 aggcgccagc ctgtaccggg ccgatccgcg cgccgcaacg caagggcccg aacccggtgc
    11341 acgccggcag gtgctggcca ggcgcgaggt gatcctctgc ggcggcgcct tcaacacgcc
    11401 gcaactgctg aagctctccg gcatcgggcc gcgtgccgag ctggagaagt tcggcattgc
    11461 ggtccgcgtg gaattgcccg gagtcggcga gaacctgcag gaccgctacg aggtcggcgt
    11521 cgccagcgag atgcgtcgcg atttccgcat cctcaacggc gcgtccttcc gcgccaccgg
    11581 ccccgacggc cagcccgatc cggcctacgt cgaatggcag cagggcaagg gcctgtacgt
    11641 cgccaacggc gcggtgatcg gcatcatcaa gcgctcgacc ccggaccgcc cggagccgga
    11701 tctctacatc ttcgggctgc cgagcttctt ccgcggctat taccccggct attccgaggt
    11761 gatcggcccg gtcaagaacc atttcacctg ggcgatcctg aaggcccata ccagtaactg
    11821 cgccggcagc gtcacgctga aatccgcgga tccgcgcgac cggcccgaca tcaacttccg
    11881 ctattttgac gaaggcaacg acgccggcca gcaggacatc gatgccgtgg tcgccggtgt
    11941 cgaattcgtg cgcgagatga acaagggcct gctgaacaag ctcgtgaccc ggcgcgaggt
    12001 cgtgcccggc cccgacatcg aatcgaagga acagatcgcg cagttcatca aggacgaggc
    12061 ctggggtcat cacgcctcgt gcacctgcag gatcggcccg tccagcgatc cgatggcggt
    12121 gctcgacagc cgcttccgcg tctacggcac gaagggcctg cgcgtcgtcg atgcctcggt
    12181 attcccgaag attccgggct tcttcatcgt cacgccggtc tacatgatca gcgagaaggc
    12241 gagcgacgtg atcctggagg acgcagaggg aatagagagg atataaatta acaagcaaag
    12301 acgagttaca cgcatggagc agacaaagag ctagcaatga aacatgctaa gcgacgtaac
    12361 tcgtcatcga caacaccagc ggcctctcaa aagctttagt ctttagcaca cgagaaaatc
    12421 aaactcaacc tttaactaaa ctggtgacaa acatgtctac tgcaccatca aagtcaaatc
    12481 tgcactggta cctcgaaata tcatatgaac tcgcttatct aggcgccaaa ttgtttggtc
    12541 tagacaaaag agcaatagat gtcatggata caggcaaaaa aaatcatgga tatcctccgc
    12601 gaggagagaa ttaaaaatta tttgtatagc acagataaaa caacaaccac aatcccccta
    12661 cccaacatca caactcctat tattgaaaat aaaagcatgc ctagcgaatc attttttcca
    12721 tcaatatcga ccctactgga tgaactagga gctgagcaaa ttcgtttttc ttttgggata
    12781 ttacagcaaa tactccaaga cggcccaatt gttgaacaat tatccggacc aacaaaaatt
    12841 atatgcttat tacctttatc acatcccata gcacttgata gttttcagcc ccttaaaaat
    12901 aaaatgacat ttaataactc aactctagca agaaatacaa cctcagacag attaaatata
    12961 agtctgcctt caattgcctt taagaccgga actggccgta aagaccacac agttgaatta
    13021 cagagaacaa aaggagagat taaagaatct tttttttgcg gccacccaag cccaagagaa
    13081 atatttaaaa ttacagttgc tcgcaccgct ttatcaaaaa tagatacgaa acgaataaaa
    13141 atatcaggca gtcgccatac agccgtgaca cttcacatcg aacaattgaa aggaggaggc
    13201 aggaacacag cacctgaaca acttgagcac gttactcgtc aatctttatg caaatttatt
    13261 ttcttagagg actaatacca tgaacactgt cccactaaag tatgtttcaa ccatgatcga
    13321 caatgaaggc aagagcatat tagaaactct ttatttactt ttcgatgagg ataaacaacc
    13381 atacgtatta ggcacagggc gatatgcaaa agtccttctt gcaagcaaag acaccgaact
    13441 taatggactc cttgctataa aattcataag caaagacgaa agcaatacgt tcaacaacac
    13501 gagtcgcgag cgattttatc aagaaataat tatcactcgc aattgcacaa gcgcacagct
    13561 taacgggaaa tttatccctt atgttggctt cggttctctg ggagagccaa ctttgccgca
    13621 acaactatct gtgattggaa atcttttcaa gatcaccaag ggattttatg atctgctttg
    13681 cttcgggtag gccatgatgc agtttgtccg agatctcagc ctctttaagt aatgcatcta
    13741 aagttgcagg tggtaatgta ttgacagttt ctttccccgc aagttcttct acgtaaagca
    13801 ctggctttag tgcgggattc ttagttcctg tacttgccca tagcggtctt tgaaccat
//
LOCUS       DAOQIE010000059        13830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2135_length_13830_cov_8.765735, whole genome shotgun sequence.
ACCESSION   DAOQIE010000059 DAOQIE010000000
VERSION     DAOQIE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13830
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2135_length_13830_cov_8.765735"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PK144_05525"
     CDS             complement(<1..1148)
                     /locus_tag="PK144_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNF65220.1"
                     /translation="MNAPEASLTAVGDAGAGAGDCFHCGLPVPPETRFHVVVDGAGRR
                     LCCHGCEAVAQAIVAAGLTDYYRHRTTISRRAEDLVPEQLRGMELYDRTDLQQSFVRA
                     EAGGTVREASLILEGIVCAACVWLNERHVGAIPGVLEFRVNYSTHRAHLRWDDARVQL
                     STVLKAIAAIGYVAHPFDPGRQEALLKRERSKALRRVAVAALGSMQVMMLAVGMYAGS
                     WYGMDEEWRHFFRWISLVIALPTVAYAGAPFFSAALRDLRNRRLGMDVPIVLAVVLAT
                     IASVWYTIFGGGEVYYDSVTMFIFFLLSARFLEMGARHRAAQVSEELTRILPATATRI
                     GPGGDEVVPVAELAAGDEVRVRPGETIPADGSVLDGASSVDESLLTGE"
     gene            complement(1145..1963)
                     /locus_tag="PK144_05530"
     CDS             complement(1145..1963)
                     /locus_tag="PK144_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNF65221.1"
                     /translation="MTDLLSSLIGGAALIALANWLLGRFTRLGPHLAGAVVALATLGI
                     YVPWAILDWPGGDLFAIHVAIYLVVSAIFALVGGARPALAGTARFPLGPSVIVGFFVL
                     LVAADSVFVTLAERGLSSPLARLLLPPTAEDARVVNSGFPGVMSHDFQKKEELYNRYL
                     EQVERQRARGWQVQKGWVSDPHAGEAARLQIAVTDRDGRPLRGALVRGEFLRPADSRL
                     DLDFEMDEVDAGVYRAAVTLPAPGTWNLVLEIRKGEDLHEVRASTRVRAGGDGS"
     gene            complement(2027..3430)
                     /gene="ccoG"
                     /locus_tag="PK144_05535"
     CDS             complement(2027..3430)
                     /gene="ccoG"
                     /locus_tag="PK144_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNF65222.1"
                     /translation="MAEEAQLYQKRLAIHPRSVEGRFRKLKTGVVLLAYGVYFLLPWL
                     RWSRPTGVNQAVLFDLPGRRFYLFDLVVQAQDIFWLAGLLVIAATLLFFVTGLAGRVW
                     CGYFCFQTLWTDVFIMIEQAIQGERPARLRLAKQPWDAEKRRKMLLTWASWLGVAFLT
                     GLTFTLYWADAPTLVGEFLTGAAPFAAYATTGFLTATTFVMAGLAREQVCTYMCPYAR
                     FQSAMFDRDTLIISYDPRRGERTAGRAKLGRDLKTRDERMAAGVGDCVDCGYCVQVCP
                     VGIDIRRGLQYQCISCALCVDACDTVMDKLEWPRGLIKYTSDNSLNDRPTRLLKPKNI
                     GYALVLMLTIAMLGWSIANRAALDAVVEQIRQPLSVQLSDGRVQNSYEIKLNSKVAQP
                     GRLRIAIEGLPGAELDMEGLETIELGAEQRLRLPAKVRAMPVAGRERQSFEFVITPLS
                     GLGTEPIRRPAAFYAGQ"
     gene            complement(3584..4519)
                     /gene="ccoP"
                     /locus_tag="PK144_05540"
     CDS             complement(3584..4519)
                     /gene="ccoP"
                     /locus_tag="PK144_05540"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNF65223.1"
                     /translation="MSDTTTTTSTPGAVQTTGHAWDGDLQEFNNPLPRWWLWSFYATV
                     VFAVFYWFAYPAWPFGKTWTKGFTTATYTEGGQSHTMRWNTRSALLEDLDSGSDAKRQ
                     RQYLDKIAQTDYAAIAKDPQMVAFVNQAGKRLFETNCSACHGRGGQGVVGLFPNLVDD
                     DWLWGGSYQAIETTLKHGRNGYMPAFGSVLNATQIDDVANYVLSLSGVVPSSEASARG
                     QKIFTGATGGCYMCHGDDGKGIKSMGAANLTDKIWTIANVPAAQSPADKLAAVRAVIG
                     SGVQNVRKMPAWGERLSAAEIKLMAVYVHQLGGGQ"
     gene            complement(4516..4737)
                     /locus_tag="PK144_05545"
     CDS             complement(4516..4737)
                     /locus_tag="PK144_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNF65224.1"
                     /translation="MFDWLSWFTRLDNSKIFSLLLFFGTFCGIVIYVFGSRRRGRRLE
                     SYKHIPFQDDEPADRHDAPAHKATDGDER"
     gene            complement(4809..5543)
                     /gene="ccoO"
                     /locus_tag="PK144_05550"
     CDS             complement(4809..5543)
                     /gene="ccoO"
                     /locus_tag="PK144_05550"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNF65225.1"
                     /translation="MRHEHIEVNSGLMIALVLAVISIGGLVEIVPLYFINDTVEKVDG
                     VRPYTPLELRGRDIYVREGCFLCHSQMIRPFRDEWLRYGHYSLAAESKYDHPFQWGSK
                     RTGPDLARVGNKFSNQWHVQHLVKPTSVVPESIMPNYPWLLDTELDYTDVADRMKALR
                     YTGVPYSLTQAEYEKNVKDFGKVVADQLDISQAKDNLMKQARTFNYDGLPDRVTEMEA
                     LVAYLQVLGTMVDFSKYDEGYFANFR"
     gene            complement(5565..7013)
                     /gene="ccoN"
                     /locus_tag="PK144_05555"
     CDS             complement(5565..7013)
                     /gene="ccoN"
                     /locus_tag="PK144_05555"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018142287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNF65226.1"
                     /translation="MAHSAAAPAEQYNYDIVRKFTIMAMVWGVLGMSAGLYAASELAW
                     PFLNFDIPQITFGRLRPVHTTLVIFGFGGCALFATSYYVVQRTCQARLPSNVLADFTF
                     YGWNVGVLLGVLSYMAGFSQGKEYAEFEWPLDLAIVAVWVVYFYNFVSVIVRRSQPHI
                     YVANWFYLAFILATAILHIFNNISVPVSLTKSYSMYAGVQDAMTQWWYGHNAVGFFLT
                     AAFLGMMYYFVPKQAGRPVYSYRLSIIHFWALIFLYMWVGAHHLHWTALPDWVSTLSA
                     TFSVLLLLPSWGGMINGIMTLSGAWDKLRSDPIMLFLITSMSFYGMSTFEGPLMSLKS
                     VNALSHYTDWTIGHVHSGALGWVALVSFGSFYHLFPRLYDTRIHSTKLIYVHFWLATV
                     GIVLYITSMWVAGIGQGLMLRGFDDYGNLAYSFIETVEFLHKPYVWRAIGGAFFVAGI
                     LLMAYNLVMTALDARRERSKLEAKLAAKLART"
     gene            complement(7284..8219)
                     /gene="cyoE"
                     /locus_tag="PK144_05560"
     CDS             complement(7284..8219)
                     /gene="cyoE"
                     /locus_tag="PK144_05560"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNF65227.1"
                     /translation="MSASPSSRLLAPGAGAGWRDYYELTKPRVVALITFTAMVGMFLA
                     TPDMVPWPVLVFGSAGIAAMAGAAAAVNHVVDRRIDAIMARTRRRPLPTGHLDGRQAL
                     AFASLLGAAGFALLLAATNTLTAWLTFASLLGYAVVYTMFLKRATPQNIVIGGAAGAA
                     PPLLGWTAVSGQVDGGALLLFLIVFVWTPPHFWALAIHRREDYARAEVPMLPVTHGVE
                     YTRLQVLLYTVVLVLVTLLPWLTGVAGPLYLAGALGLGARFLWLAARLYLSREDRHAL
                     PVFLYSIVYLFALFAVLMVDHYLFTILKQVQRLVI"
     gene            complement(8216..9280)
                     /locus_tag="PK144_05565"
     CDS             complement(8216..9280)
                     /locus_tag="PK144_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNF65228.1"
                     /translation="MQTLSPAVSLTSAAAPAVPGRRLAFSQLAIAACVLALCVVVLGA
                     YVRLSHAGLGCPDWPGCYGHLDVPESVRHIEAANAAWPDRPVEPEKAWKEMIHRYAAG
                     TLGLLILALAVSAWRRRHEPGQMLALPLALVALVLFQALLGMWTVTLLLKPLIVLGHL
                     LGGLATLGLLFWLALRQSGWQPRPVEAERRRLGGWALLALVVLIVQIALGGWTSTNYA
                     ALACPDLPTCQGRWWPPTDFADAYLPWRGLGINYEGGVLDNEARMTIHVMHRVGALLA
                     LAVLGALAWRLARSLDAGLRRGGWLLFAALVAQLLLGFGNVLLSLPLPVAVAHNAGAA
                     VLLLVVLGLNHLLHPRRLPA"
     gene            complement(9286..9909)
                     /locus_tag="PK144_05570"
     CDS             complement(9286..9909)
                     /locus_tag="PK144_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase assembly protein"
                     /protein_id="HNF65229.1"
                     /translation="MNPELTLLQLRPASVRRARLQLLLIIALFALPLAVAISLFGQWR
                     PAGSAEHGELLNPVRALSPLSALATDDTPLAPDFLHGRWTLVYVASGSDCPALCRDAL
                     WRLRQVRLAIGRDIDRVQRLVLFASMPDDASRQWLAREHPGLAAGAATTEARTEFTGG
                     WRTDGRPGGWIYLVDPHGNLVLRYAADGEAGGMLKDLTRLLKLSQIG"
     gene            complement(9906..10649)
                     /locus_tag="PK144_05575"
     CDS             complement(9906..10649)
                     /locus_tag="PK144_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNF65230.1"
                     /translation="MPPRRLGWLLLTCAVAAVFVSLGCWQLRRADEKSAWLADWDARS
                     HRPAVDLATLDPAAAGSRYRRVELRGEYRAGHEFLLLNRMRDGRVGIEAYTPFVPAGG
                     GDWLLVNRGWYESPQARPKTPDAPPAGVQTIRGLLAQPANPGLRLGTIEVAGPWPQPV
                     VWLDAPAAAHALGHGVLGAVLLLDAGLPGGFVRDWHPDPGEFGPERHRGYALQWFALA
                     ATVVCTWIVLSRRQRRRDSLPEARTSPSA"
     gene            10725..10931
                     /locus_tag="PK144_05580"
     CDS             10725..10931
                     /locus_tag="PK144_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HNF65231.1"
                     /translation="MLFRVLVIAALLAIVASLGSGLFFLVRERERSERLVRALTVRIA
                     LSIGLFVLLLLAWAAGLVQPHGVH"
     gene            complement(10950..11831)
                     /locus_tag="PK144_05585"
     CDS             complement(10950..11831)
                     /locus_tag="PK144_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF65232.1"
                     /translation="MSTTTHDAPSAGSYYVPHSSPWPIIGSVGLFVTMMGGANLLNGH
                     APTLLYCGLAVLIVMMIGWFNNVIRESQAGLYSPQMDRSFRWGMSWFIFSEVMFFAAF
                     FGALFYARDYALPWLDGTSASKPMTHELLWPTFEERWPTNGPGRVGGTFEVMGPWGLP
                     ALNTLILLSSGATVTWAHWGLLKGNRRQLCLGLAATVGLGFLFVALQAVEYSHAYQHM
                     NLTLGSGIYGSTFFMLTGFHGAHVTIGAIMLTVMLLRSLAGHFTPEHHFAFEAAAWYW
                     HFVDVVWLGLFVFVYWF"
     gene            complement(11857..12405)
                     /locus_tag="PK144_05590"
     CDS             complement(11857..12405)
                     /locus_tag="PK144_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNF65233.1"
                     /translation="MSPPVPAAAAGVQRSIRRTLLVVVAMFGFGFALVPLYDVLCQVT
                     GLNGKTRAAVADAAPMPVDEARTVIVEFIASVNDEAPWEFRPDVTRIEVHPGRFYTTH
                     FYARNRSETPLAGQAVPSVAPGLASLHFHKIECFCFNQQRFAPGEGREMPVTFRIDPQ
                     LPAEVGTVTLSYTFFRLPGQGG"
     gene            complement(12402..12539)
                     /locus_tag="PK144_05595"
     CDS             complement(12402..12539)
                     /locus_tag="PK144_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65234.1"
                     /translation="MEAAMNPGLPDPQPRRRAVRRTVITLVVIAVAFYAGFIWLAAQR
                     A"
     gene            complement(12539..>13830)
                     /locus_tag="PK144_05600"
     CDS             complement(12539..>13830)
                     /locus_tag="PK144_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HNF65235.1"
                     /translation="GAPDMALPRMNNWSFWIMPFAFALLLSTLFMDGGGPAGGWTLYP
                     PLSLQTGAAFPFVILSIHMMGVSSVMGAINIIATILNLRAPGMTLMRMPLFVWTWLIT
                     AYLLIAVMPVLAGAVTMLLTDKYFGTSFFNAAGGGDPVMFQHIFWFFGHPEVYIMILP
                     AFGIVSQIIPTFARKPLFGYASMVYATASIAFLSFIVWGHHMFTVGMPLAGELFFMYA
                     TMLIAVPTGVKVFNWISTMWRGSMTFETPMLFAIAFVVLFTIGGFSGLMLAIAPADFQ
                     YQDTYFVVAHFHYVLVPGAVFSIMAAAYYWLPKWTGHMYDEKLGRLHFWCSAIGVNIL
                     FFPQHFLGLAGMPRRIPDYALQFAEFNMISSIGAFIFGFSQLIFLWVVIKCVRGGEPA
                     PAKPWEGAHGLEWTVPSPAPHHTFQTPPQITEAEAHS"
BASE COUNT         2396 a         4678 c         4821 g         1935 t
ORIGIN      
        1 ctctcgccgg tcagcagcga ttcatcgacg ctgctcgccc cgtcgagcac gctgccatcg
       61 gccggaatcg tctcgccggg ccgcacgcgt acctcgtcgc cggccgcgag ttcggccacc
      121 ggcacgacct cgtcgcctcc gggaccgatg cgggtcgcgg tcgccggcag gatgcgggtg
      181 agttcctcgg acacctgcgc ggcgcggtgg cgggcgccca tttccaggaa gcgcgcgctg
      241 agcaggaaga agatgaacat cgtcaccgag tcgtaataga cctcgccgcc gccgaagatc
      301 gtgtaccaga cgctggcgat ggtcgcgagc acgacggcga gcacgatcgg cacgtccatg
      361 ccgaggcggc ggttgcgcag atcgcgcagc gcggcgctga agaacggcgc accggcatag
      421 gcgacggtcg gcagcgcgat cacgagactg atccagcgga agaaatgccg ccattcctcg
      481 tccatgccgt accagctgcc ggcgtacatg ccgacggcga gcatcatgac ctgcatcgag
      541 ccgagcgcgg cgaccgcgac ccggcgcagc gccttcgagc gctcgcgctt gagcagcgcc
      601 tcctgccggc ccggatcgaa cggatgcgcg acgtagccga tcgcggcgat cgccttcagg
      661 accgtgctca gctgcacgcg ggcatcgtcc cagcgcaggt gcgcgcggtg ggtcgagtag
      721 ttgacgcgga attccagcac gcccggaatc gcgccgacgt ggcgttcgtt gagccagacg
      781 caggccgcac agacgatgcc ttcgaggatc agcgaggcct cgcgcaccgt gccgccggcc
      841 tcggcgcgca cgaagctctg ctgcaggtcg gtgcggtcgt agagctccat gccgcgcagc
      901 tgctcgggca cgagatcctc ggcccggcgc gagatcgtcg tgcggtggcg gtagtagtcg
      961 gtcagcccgg ccgcgacgat cgcctgcgcg accgcctcgc agccgtggca gcagagccgc
     1021 cgcccggcgc cgtcgacgac gacgtggaag cgcgtttccg gcggcaccgg caggccgcag
     1081 tggaagcagt cgccggcgcc ggcaccggca tcgccgacgg cagtcaggct ggcctcgggg
     1141 gcgttcatga accgtctccg ccggcgcgca cgcgcgtgct ggcgcgcacc tcgtgcaggt
     1201 cctcgccctt gcggatttcc agcaccagat tccaggtgcc gggggccggc agcgtcacgg
     1261 cggcgcgata gacgccggca tcgacctcgt ccatctcgaa gtcgagatcg agccggctgt
     1321 cggccgggcg caggaactcg ccgcgcacga gcgccccgcg cagcggccgg ccgtcgcggt
     1381 cggtgacggc gatctgcagc cgcgccgcct cgccggcgtg cgggtccgag acccagccct
     1441 tctgcacctg ccagccgcgt gcgcgctggc gctcgacctg ttcgaggtag cggttgtaca
     1501 gctcttcctt cttctggaag tcgtgcgaca tgacgccggg gaagccggag ttgaccacgc
     1561 gcgcgtcctc ggcggtcggc ggcagcagca gccgcgccag cggcgaggac aggccgcgtt
     1621 cggccagcgt cacgaacacc gagtcggccg cgaccagcag cacgaagaag ccgacgatca
     1681 cgctcggacc gagcgggaag cgcgccgtgc cggcgagtgc cgggcgcgcg ccgccgacca
     1741 gcgcgaagat cgcggacacg accagataga tcgcgacgtg gatcgcgaac aggtcgcccc
     1801 cgggccagtc gaggatcgcc cagggcacgt agatgccgag cgtcgcgagg gcgacgacgg
     1861 cgccggcgag gtgcggaccg aggcgggtga agcgcccgag cagccagttg gccagcgcga
     1921 tcagcgccgc accgccgatc agcgaggaca gcagatcagt catgtcacga ttcctgccgg
     1981 tgtgctcgtc gccaccggcc ccgggccgcg tgccggcgca ccggcgtcac tggcccgcat
     2041 agaaggccgc cgggcggcgg atcggttcgg ttccgagccc cgacagcggc gtgatgacga
     2101 actcgaagct ctggcgctcc cggccggcga ccggcatggc gcgcaccttc gccggcaggc
     2161 gcaggcgctg ttcggcaccg agttcgatgg tttcgagccc ctccatgtcg agctcggctc
     2221 cgggcaggcc ctcgatcgcg atgcgcaggc gcccgggctg cgcgaccttg ctgttgagct
     2281 tgatctcgta gctgttctgc acgcggccat cggagagctg caccgacagc ggctggcgga
     2341 tctgctcgac cacggcatcg agggcggcgc ggttggcgat gctccagccg agcatcgcga
     2401 tcgtcagcat cagcaccagc gcgtagccga tgttcttcgg cttcagcagc cgggtcgggc
     2461 ggtcgttgag cgagttgtcg gaggtgtact tgatcagccc gcgcggccat tcgagcttgt
     2521 ccatgaccgt gtcgcaggca tcgacgcaca gcgcgcagga gatgcactgg tactgcaggc
     2581 cgcggcggat gtcgatgccg accgggcaga cctgcacgca gtagccgcag tcgacgcagt
     2641 cgccgacgcc ggcggccatg cgctcgtccc gggtcttgag atcgcgcccc agcttggcgc
     2701 gaccggccgt gcgctcgccg cggcgcgggt cgtaggagat gatcagcgtg tcgcgatcga
     2761 acatcgcgct ctggaagcgc gcgtacggac acatgtaggt gcagacctgc tcgcgcgcga
     2821 gcccggccat cacgaaggtg gtcgcggtca ggaagccggt ggtcgcatag gccgcaaacg
     2881 gcgcggcccc ggtcaggaac tcaccgacca gcgtcggtgc atcggcccag tacagcgtga
     2941 aggtcaggcc ggtcaggaag gccacgccca gccaggacgc ccaggtcagc agcatcttgc
     3001 ggcgcttctc ggcatcccag ggctgcttcg ccagccgcag gcgcgccggg cgttcgccct
     3061 ggatcgcctg ctcgatcatg atgaacacat cggtccagag cgtctggaag cagaagtagc
     3121 cgcaccagac gcgcccggcc agaccggtca cgaagaacag cagcgtcgcg gcgatcacca
     3181 gcaggccggc gagccagaag atgtcctgcg cctgcacgac cagatcgaac aggtagaagc
     3241 gccggcccgg caggtcgaac agcaccgcct ggttgacgcc ggtcggccgc gaccagcgca
     3301 gccacggcag caggaagtag acaccgtagg cgagcagcac gacgccggtc ttgagcttgc
     3361 ggaaccgccc ttcgacggag cggggatgga tcgccagacg cttctggtag agctgtgctt
     3421 cttcggccat ggactttccg ggtggtcgta acggcacggc gaccgcagcg caggcactgc
     3481 ggtcgccgcc ggaggtggga cggagccgtc aagcatcgcc gccgggcggc gccagggcac
     3541 tgacggttcg cactcgtccg ctgccggaac ccgtccggct tgcttactgg ccgccgccga
     3601 gctgatgcac gtagaccgcc atcagcttga tctcggcggc actgaggcgc tcgccccagg
     3661 ccggcatctt gcgcacgttc tgcacgccgc tgccgatcac cgccctgacc gccgcgagct
     3721 tgtcggcggg gctctgggca gccgggacgt tggcgatcgt ccagatcttg tcggtcaggt
     3781 tcgccgcacc catcgacttg atgcccttgc cgtcgtcgcc gtggcacatg tagcagccgc
     3841 cggtggcgcc ggtgaagatc ttctggccgc gggcgctggc ctcgctggac ggcacgacgc
     3901 cggacagcga cagcacgtag ttcgcgacat cgtcgatctg cgtggcgttc agcacgctgc
     3961 cgaaggccgg catgtagccg ttgcggccgt gcttgagcgt cgtctcgatg gcctggtagc
     4021 tgccgcccca cagccagtcg tcgtcgacga ggttcgggaa cagcccgacc acgccctgac
     4081 cgccgcggcc atggcaggcc gagcagttgg tctcgaacag gcgcttgccg gcctggttca
     4141 cgaacgcgac catctgcggg tccttggcga tcgccgcgta atcggtctgc gcgatcttgt
     4201 cgaggtactg gcgctggcgc ttggcatccg agccgctgtc gaggtcttcg agcagggcac
     4261 tgcgcgtgtt ccagcgcatc gtgtgcgact ggccgccctc ggtgtaggtc gcggtcgtga
     4321 agcccttggt ccaggtcttg ccgaagggcc aggccggata cgcgaaccag tagaacaccg
     4381 caaagaccac ggtcgcgtag aacgaccaca gccaccagcg cggcagcgga ttgttgaact
     4441 cctgcaggtc accgtcccag gcgtgaccgg tggtctgcac cgccccgggc gtgctcgttg
     4501 tcgtcgtcgt gtcactcatc gttcgtcacc gtccgtcgcc ttgtgcgcgg gcgcgtcatg
     4561 ccggtcagcc ggctcgtcgt cctgaaacgg aatgtgcttg tacgactcca gccggcggcc
     4621 gcggcggcgg ctgccgaaga cgtagatcac gattccgcag aaggtcccga agaacagcag
     4681 cagcgagaag atcttgctgt tgtcgagacg ggtgaaccag gacagccagt cgaacacgtc
     4741 gcgcctaccg aatcatccgg gcccgccccg tcgccggagc gcggcccgtt cgccgaatca
     4801 ccgaatgctc agcggaagtt cgcgaagtag ccttcgtcgt acttgctgaa gtcgaccatc
     4861 gtgccgagca cctgcaggta ggcgaccagc gcctccatct cggtgacgcg gtccggcagg
     4921 ccgtcgtagt tgaaggtgcg cgcctgcttc atcaggttgt ccttggcctg gctgatgtcg
     4981 agctggtcgg ccaccacctt gccgaagtcc ttgacgttct tttcgtactc ggcctgcgtc
     5041 agcgagtagg gcacgccggt gtagcgcagc gccttcatgc gatcggcgac atcggtgtag
     5101 tcgagttcgg tgtcgagcag ccacgggtag ttcggcatga tcgactcggg cacgaccgag
     5161 gtcggcttga ccagatgctg cacgtgccac tggttcgaga acttgttgcc gacgcgcgcc
     5221 agatccggac cggtgcgctt cgagccccac tggaacgggt ggtcgtactt gctctcggcg
     5281 gccagcgagt agtggccgta gcgcagccac tcgtcacgga acggccggat catctgcgag
     5341 tggcacagga agcagccctc gcgcacgtag atgtcgcgac cgcgcagttc gagcggcgtg
     5401 tacgggcgca cgccgtcgac cttctcgacg gtgtcgttga tgaagtacag gggcacgatc
     5461 tcgaccagac cgccgatgct gatgacggcc agcaccagcg cgatcatcag gccggagttg
     5521 acctcaatat gctcgtgtct catgtcgctt ggactccagt cggatcaggt gcgggccagc
     5581 ttggccgcga gcttggcttc gagcttgctg cgctcgcgac gcgcatcgag cgcggtcatc
     5641 acgaggttgt aagccatcag caggatgccg gcgacgaaga acgcaccgcc gatggcgcgc
     5701 cacacgtagg gcttgtgcag gaactcgacg gtctcgatga agctgtaggc gaggttgccg
     5761 tagtcatcga agccacgcag catcaggccc tggccgatgc cggcgaccca catcgaggtg
     5821 atgtacagca cgatgccgac cgtcgcgagc cagaagtgca cgtagatcag cttggtgctg
     5881 tggatgcgcg tgtcgtacag gcgcgggaac aggtggtaga acgaaccgaa gctgaccagc
     5941 gccacccagc cgagcgcacc ggagtgcacg tggccgatgg tccagtcggt gtagtgcgac
     6001 agcgcgttga cgctcttcag cgacatcagc ggaccttcga aggtcgacat gccgtagaag
     6061 gacatcgagg tgatcaggaa cagcatgatc gggtcggagc gcagtttgtc ccaggccccc
     6121 gacagcgtca tgatgccgtt gatcatgccg ccccaggacg gcagcagcag cagcaccgag
     6181 aaggtcgccg acagcgtcga gacccagtcc ggcagcgcgg tccagtgcag gtggtgcgca
     6241 ccgacccaca tgtacaggaa gatcagcgcc cagaagtgga tgatcgacag ccggtacgag
     6301 tacaccggcc ggccggcctg cttcggcacg aagtagtaca tcattccgag gaaggccgcg
     6361 gtcaggaaga agccgaccgc gttgtggccg taccaccact gcgtcatcgc gtcctgcacg
     6421 ccggcgtaca tcgagtacga cttggtcagc gacaccggca ccgagatgtt gttgaagatg
     6481 tgcaggatcg ccgtcgcgag gatgaaggcg agatagaacc agttcgcgac atagatgtgc
     6541 ggctgcgagc ggcgcacgat cacggacacg aagttgtaga agtacacgac ccagacggcg
     6601 acgatcgcca ggtccagcgg ccactcgaac tcggcgtact ccttgccctg cgagaagccg
     6661 gccatgtagc tcagcacgcc gagcagcacg ccgacgttcc agccgtagaa cgtgaagtcc
     6721 gccagcacgt tgctcggcag gcgcgcctga caggtacgct gcacgacgta gtacgaggtg
     6781 gcgaacagcg cacagccgcc gaagccgaag atcacgagcg tcgtgtgcac cggacgcagg
     6841 cgaccgaagg tgatctgcgg gatgtcgaag ttcaggaagg gccaggccag ctcgctggcg
     6901 gcgtagaggc cggcactcat gccgagcaca ccccacacca tggccatgat cgtgaacttg
     6961 cgcacgatgt cgtagttgta ctgctcggcg ggcgccgctg cgctatgggc catatcccga
     7021 tactcccgat gctgcaggct ggacagaaaa cgaacgggcc ctgccgggtg cggtgctccg
     7081 gcagggctct cgccgcgggg tcttgagcgg cccggcatgt tacttgaggt taatcaattc
     7141 cctcaatcgc agtttggtct ccgtgacgca tttgccccgc gatgcgaaat gccgccgcca
     7201 ggcatgttca cgtccatacc ctcaaagcgg aaacccccgc ttccggggcc cggaagcggg
     7261 ggtggcggca cggtgcggcg tgctcagatc accagccgct gcacctgctt caggatcgtg
     7321 aacaggtagt gatcgaccat cagcaccgcg aacagcgcga acaggtagac gatcgagtac
     7381 aggaacaccg gcagtgcgtg ccggtcctcg cggctcaggt acagccgcgc ggcgagccac
     7441 aggaagcgcg cgccgagccc gagcgcgccg gcgaggtaca gcggcccggc gacaccggtc
     7501 agccacggca gcagcgtcac cagcaccagc acgaccgtgt acagcagcac ctgcaggcgc
     7561 gtgtactcga ccccgtgcgt gaccggcagc atcggcactt cggcgcgcgc gtagtcctcg
     7621 cgccggtgga tcgccagcgc ccagaagtgc ggcggcgtcc acacgaacac gatcaggaac
     7681 agcagcagcg ccccgccgtc gacctgcccg ctcaccgccg tccagccgag cagcggcggc
     7741 gcggcgccgg ccgcgccgcc gatgacgatg ttctgcggcg tcgcgcgctt gaggaacatc
     7801 gtgtagacga ccgcgtagcc gagcagcgac gcgaaggtca gccacgcggt cagcgtgttg
     7861 gtcgccgcaa gcagcagcgc gaagccggcc gcgccgagca ggctcgcgaa ggcgagcgcc
     7921 tgacggccgt cgagatgccc ggtcggcagc ggccggcggc gcgtgcgcgc catgatcgcg
     7981 tcgatgcgcc ggtcgacgac gtggttgacc gccgccgccg cgccggccat cgccgcgatg
     8041 ccggccgagc cgaacaccag caccggccac ggcaccatgt ccggggtcgc gaggaacatg
     8101 ccgaccatgg ccgtgaacgt gatcagcgcg acgacgcgcg gcttggtcag ctcgtagtaa
     8161 tcgcgccagc cggcaccggc gccgggcgcc agcagccggg acgaagggga tgcgctcatg
     8221 cgggcagtct ccgcggatgc agcaggtggt tcaggccgag cacgaccagc agcagtacgg
     8281 ccgccccggc gttgtgggcg accgcgaccg gcagcggcag cgacagcagc acgttgccga
     8341 agccgagcag caactgcgcc acgagcgcgg cgaacagcag ccagccgcca cggcgcaggc
     8401 cggcatcgag gcttcgcgcc agccgccacg ccagcgcgcc gagcacggcg agtgcgagca
     8461 gcgcgccgac gcggtgcatg acgtggatgg tcatgcgcgc ctcgttgtcg agcacgccgc
     8521 cctcgtagtt gatgccgagt ccgcgccacg gcaggtaggc atcggcgaaa tcggtcggcg
     8581 gccaccagcg gccctgacag gtcggcaggt ccgggcaggc gagcgccgca tagttggtgc
     8641 tggtccagcc gccgagcgcg atctgcacga tcagcaccac gagcgccagc agcgcccagc
     8701 cgccgagccg gcggcgttcg gcttcgaccg gacgcggctg ccagccggac tggcgcagcg
     8761 ccagccagaa cagcagcccg agcgtcgcca gcccgccgag caggtgtccg agcacgatca
     8821 gcggcttcag cagcagtgtc acggtccaca tgccgagcag cgcctggaac agcaccaggg
     8881 cgaccagcgc cagcggcagg gccagcatct gccccggctc atgccgccgg cgccaggcac
     8941 tcacggcgag cgcgaggatc agcagcccga gcgtgccggc cgcatagcgg tggatcatct
     9001 ccttccaggc cttctccggc tcgaccgggc gatccggcca cgcggcgttg gcggcctcga
     9061 tgtgccggac cgattccggt acatcgaggt ggccgtagca gcccggccag tccggacagc
     9121 cgaggccggc gtgcgacagg cgcacatatg caccgagcac gaccacgcac agcgccagca
     9181 cgcaggccgc gatggccagc tgcgaaaacg ccagccgccg gcccggcacg gcaggcgctg
     9241 cggcggaggt cagggaaact gcgggcgaca gggtctgcat gggaatcagc cgatctgcga
     9301 gagcttcaac aagcgggtca ggtccttcag catgccgccg gcctcgccgt cggcggcgta
     9361 gcgcaacacc agattgccgt gcggatcgac gaggtagatc cagccgccgg gacgcccgtc
     9421 cgtgcgccag cccccggtga attccgtgcg ggcctcagtc gtggccgcgc cggctgcaag
     9481 ccccggatgc tcgcgcgcca gccactggcg gctcgcgtca tcgggcatcg aggcgaacag
     9541 caccaggcgc tgcacgcggt cgatgtcgcg gccgatggcg aggcgcacct gacgcagccg
     9601 ccacagcgca tcgcgacaga gcgccgggca gtcgctgccg gaggccacat acaccagcgt
     9661 ccagcgcccg tgcaggaagt ccggcgcgag cggcgtgtcg tcggtggcga gcgccgacag
     9721 cggtgacagc gcgcgcaccg ggttcagcag ttcgccgtgc tcggcgctgc cggccgggcg
     9781 ccactggccg aacaggctga tcgccaccgc cagcggcaag gcgaacagcg cgatgatcag
     9841 cagcagttgc agacgcgcgc gacgcacact cgcggggcga agctgcagca gggtcaattc
     9901 cggattcatg cggacggact cgttcgggcc tcgggaaggg aatcgcgccg gcgctgccgg
     9961 cggctcagga cgatccaggt acagacgacg gtcgccgcca gcgcgaacca ctgcagcgca
    10021 tagccgcggt ggcgctcggg gccgaactcg ccgggatccg gatgccagtc acggacgaag
    10081 ccgcccggca gcccggcatc gagcagcagc acggctccga gcacgccgtg cccgagcgcg
    10141 tgcgccgcgg cgggggcatc gagccagacc accggctgcg gccacggccc ggccacttcg
    10201 atcgtgccga ggcgcaggcc cggattggcc ggctgcgcga gcaggccgcg gatcgtctgc
    10261 acgccggccg gtggagcgtc gggggtcttc gggcgggcct gcggactctc gtaccagccg
    10321 cggttgacca gcagccagtc accgccgccg gccggcacga agggcgtgta ggcctcgatg
    10381 ccgacgcgac cgtcgcgcat gcggttcagc agcaggaact cgtgcccggc gcggtattca
    10441 ccgcgcagtt cgacgcgccg gtaacggctg ccggccgcgg ccggatcgag cgtggccaga
    10501 tcgacggccg ggcggtgcga acgcgcgtcc cagtcagcaa gccacgcgct cttctcgtcg
    10561 gcccggcgca gctgccagca gccgagcgac acgaagaccg cagcgacggc gcaggtcagc
    10621 agcagccagc cgaggcggcg cggcggcatc agcgtggctc ctgccgggtg cggccggcgc
    10681 gtagactcgg gcgcaagcac ctgcggagtc ccttcatgtc catcgtgctg ttccgcgttc
    10741 tcgtcatcgc cgcgctgctc gcgatcgtcg cgagcctcgg ctccgggctg ttctttctgg
    10801 tgcgcgagcg cgagcgcagc gagcggctgg tgcgcgcgct gaccgtgcgc atcgcgctgt
    10861 cgatcggcct gttcgtgctg ctgctgctcg cctgggccgc cggtctggtc cagccgcacg
    10921 gcgtgcactg atgacggacg ggcaccggct cagaaccagt acacgaacac gaacagtccg
    10981 agccagacca cgtcgacgaa gtgccagtac caggccgcgg cctcgaacgc gaagtggtgc
    11041 tcgggcgtga agtgcccggc cagcgagcgc agcagcatga ccgtcagcat gatcgcgccg
    11101 atggtcacgt gcgccccgtg gaagccggtc agcatgaaga aggtcgagcc gtagatgccg
    11161 ctgccgagcg tcaggttcat gtgctggtag gcatggctgt actcgaccgc ctgcagcgcg
    11221 acgaacagga agccgagtcc gacggtcgcg gcgagcccga ggcagagctg gcggcggttg
    11281 cccttcagca gtccccagtg cgcccaggtc acggtcgcgc cgctcgacag caggatcagc
    11341 gtgttcagcg ccggcaggcc ccacggcccc atgacctcga aggtgccgcc gacgcggccc
    11401 gggccgttgg tcggccagcg ctcctcgaag gtcggccaca gcagttcgtg cgtcatcggc
    11461 ttggaagcgg acgtgccgtc gagccacggc agcgcgtagt cacgcgcgta gaacagcgcg
    11521 ccgaaaaagg ccgcgaagaa catgacttcc gagaagatga accagctcat gccccagcgg
    11581 aacgagcggt ccatctgcgg gctgtagagg ccggcctggc tctcgcggat gacgttgttg
    11641 aaccagccga tcatcatcac gatcagcacc gccagcccgc agtacagcag cgtcggcgcg
    11701 tggccgttca gcaggttggc gccgcccatc atcgtgacga acaggccgac cgaaccgatg
    11761 atcggccagg ggctgctgtg cggcacgtaa tagctgccgg cggatggcgc gtcgtgcgtc
    11821 gtcgtgctca tggatcgccc tcggggatgt tgcgcttcag ccgccctgcc cgggcagccg
    11881 gaagaaggtg taggacagcg tcacggtgcc gacctcggcc ggcaactgcg gatcgatgcg
    11941 gaaggtcacc ggcatctcgc gaccctcgcc gggcgcgaaa cgctgctggt tgaagcagaa
    12001 gcattcgatc ttgtggaaat gcagcgaggc cagccccggc gcgacactcg gcacggcctg
    12061 gccggccagc ggcgtttcac tgcggtttcg cgcgtagaag tgcgtcgtgt agaagcgccc
    12121 cggatgcacc tcgatgcgcg tgacatccgg gcggaactcc cacggcgcct cgtcgttgac
    12181 gctggcgatg aactcgacga tcaccgtgcg cgcctcatcg accggcatcg gcgcggcatc
    12241 ggcaaccgcc gcacgcgtct tgccgttgag gccggtgacc tgacagagca cgtcgtacag
    12301 cggcaccagc gcgaagccga agccgaacat cgccacgacg acgagcagcg tgcgccggat
    12361 cgagcgctgc acgccggcgg ccgcggccgg gaccggcggg ctcatgcgcg ctgcgccgcc
    12421 agccagatga agccggcgta gaacgccacg gcgatcacga ccagcgtgat gacggtgcgc
    12481 cgcacggcgc ggcggcgcgg ctgcggatcg ggcaggccgg ggttcatcgc ggcctccatc
    12541 agctgtgcgc ctccgcctcg gtgatctgcg gcggcgtctg gaaggtgtgg tgcggcgccg
    12601 gcgacggcac cgtccattcc agcccgtgcg caccctccca gggcttggcc ggtgccggct
    12661 cgccgccgcg cacgcacttg atcacgaccc acaggaagat cagctgcgag aagccgaaga
    12721 tgaacgcgcc gatcgacgag atcatgttga actcggcgaa ctgcagcgcg tagtcgggga
    12781 tgcgccgcgg catgccggcg agtccgagga agtgctgcgg gaagaacagg atgttgacgc
    12841 cgatcgccga gcaccagaaa tgcagccggc cgagcttctc gtcgtacatg tggccggtcc
    12901 acttcggcag ccagtaatac gcggcggcca tgatcgagaa caccgcgccc ggcaccagca
    12961 cgtagtggaa gtgtgcgacg acgaagtagg tgtcctgata ctggaagtcg gccggcgcga
    13021 tcgcgagcat cagacccgag aagccgccga tcgtgaacag cacgacgaac gcgatcgcga
    13081 acagcatcgg cgtctcgaag gtcatcgagc cgcgccacat cgtgctgatc cagttgaaca
    13141 ccttcacgcc ggtcggcacc gcgatcagca tcgtcgcgta catgaagaac agttcgccgg
    13201 cgagcggcat gccgaccgtg aacatgtggt ggccccagac gatgaagctc aggaacgcga
    13261 tcgacgcggt cgcgtagacc atcgacgcat agccgaacag cggcttgcgt gcgaaggtcg
    13321 ggatgatctg cgacacgatc ccgaaggccg gcaggatcat gatgtagacc tcgggatgcc
    13381 cgaagaacca gaagatgtgc tggaacatca ccgggtcgcc gccgccggcg gcgttgaaga
    13441 agctggtgcc gaagtacttg tcggtcagca gcatggtcac ggccccggcc agcaccggca
    13501 tcaccgcgat cagcaggtag gcggtgatca gccaggtcca cacgaacagc ggcatgcgca
    13561 tcagcgtcat gcccggcgcg cgcaggttca ggatcgtggc gatgatgttg atcgcgccca
    13621 tcaccgacga cacgcccatc atgtgaatcg acaggatcac gaacgggaag gccgcgccgg
    13681 tctgcaggct cagcggcggg tacagcgtcc agccgccggc cgggccgccg ccgtccatga
    13741 acagcgtcga caacagcagc gcgaacgcga acggcatgat ccagaacgac cagttgttca
    13801 tgcgcggcag cgccatgtcc ggcgcgccga
//
LOCUS       DAOQIE010000060        13786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2147_length_13786_cov_7.691501, whole genome shotgun sequence.
ACCESSION   DAOQIE010000060 DAOQIE010000000
VERSION     DAOQIE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_13786_cov_7.691501"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="dapC"
                     /locus_tag="PK144_05605"
     CDS             complement(<1..971)
                     /gene="dapC"
                     /locus_tag="PK144_05605"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNF65236.1"
                     /translation="MNPDLDRLQPYPFEKLARLKSGLHAPHGLAHIPLYIGEPQHPAP
                     QLALDALVANLGALSNYPLTRGTPALRAAIADWAVRRYRLTGLDADRQVLPVAGTREA
                     LFAFAQAVVARSDTAAGRPLVLMPNPFYQIYEGAALLAGAEPYFLNTPAGTGFLPDYD
                     AVPEEVWARCQLLYLCSPGNPTGAVTPAATLAKLIDKADEFGFVIAADECYSEIYFDE
                     AAPPVGLLEACARLGRHDYRRCVVFNSLSKRSNLPGLRSGFVAGDADVLAAFLQYRTY
                     HGCALPGSTQAASVAAWGDEAHVQANRALYRAKLDAVLDVLGPVLDVS"
     gene            1119..1664
                     /locus_tag="PK144_05610"
     CDS             1119..1664
                     /locus_tag="PK144_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF65237.1"
                     /translation="MSIARLLSAAALAALLSACMPLSPLPAEAPADATAVSGRLPKAD
                     LILVKKSDRRLTLLRNGREIKSYKVSLGRNPVGQKVAAGDGRTPEGDYLIDWRKPDSK
                     FYKALHISYPRVSDRRTAEEAGRNPGGAIMIHGLPNDRPRAHHYLPGIDWTEGCIAVT
                     NREMDEIWHSVRDGTPIRILP"
     gene            1752..3698
                     /gene="recD"
                     /locus_tag="PK144_05615"
     CDS             1752..3698
                     /gene="recD"
                     /locus_tag="PK144_05615"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNF65238.1"
                     /translation="MTESAAPDTDPRARVDDELDRLLAAGAIGPLDREFARLLQRLVP
                     KPAPRAGLLAAALASRQIGLGHVCLPLTGFEGFRRRLLAEDEQGPAIWAPAAAEWIER
                     LRRWPAVGSPGEFRPLVLDADGRLYLYRYWRYETGLAARLVELAQAPVRVDEPRLRQG
                     LDRLFPQAAADAAGPDLQRLACANAALSRLALICGGPGTGKTTTLAKLLALLLMHDGD
                     SLRIRLAAPTGKAAARMLEALRGARDRLAGQLEPALLAALPTDASTLHRLLGVRPDST
                     ARFRHDAGHPLALDVLVIDEVSMVDLPLLAKTVAALPAHARLILLGDQDQLASVEAGA
                     VLGELGAQAGAPSPQRAAQLAAISGCTIEADPEPLLPPLRDCVALLRRSYRFAGASGI
                     GALARAVNAGDAGAALAQLEAGAEDLQWSQATAIEAVIERARSGYRSYLQLLGTAAAA
                     QDPQALHAAFARFRVLGGLRSGALGVETLNARLEREARRAAGRPDTALWYPGRPVMVL
                     RNDYGLNLYNGDIGIATVDAEGTLAVQFPRPEGGFRALPVARLPEHEPVFAMTVHKSQ
                     GSEYDEVLLVAPPADSPLATRELLYTGLTRARAQLGIVASAEAVQVACSRRVARDSGL
                     AGRLAEAAAGTLVQPPGVSPRTPG"
     assembly_gap    3774..3873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4077..4559
                     /locus_tag="PK144_05620"
     CDS             4077..4559
                     /locus_tag="PK144_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF65239.1"
                     /translation="MAYSIGTILYATDLGPRSLEVFQHAVGYARQFGAKMHVITCIEA
                     IGDYANVTLDTYVPAEVIAQIREDGIKRINEEIETRFNRHDAAHPDNTCREVIASVQI
                     IEGSAADVVLSEQKRLNADLIVLGSHGHTALGEMLIGSVAHKVTVKSPVPVLLVPINQ
                     "
     gene            4837..5982
                     /locus_tag="PK144_05625"
     CDS             4837..5982
                     /locus_tag="PK144_05625"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HNF65240.1"
                     /translation="MSSPFSVRCARSGLVIATLLLPVLPVQALTFNFIADAGMDAQAY
                     DAFVRAGNRWSSVFTDPVTVNLRIGYSALASGVLAQASSTQTIVPYTNFVSALRADRT
                     SSQDTTAVSNLSADLKFLTNRTSDSPNGPGSATPYLDNDGSFNNQYLRMTSANAKALG
                     LMSATATGTDATITFSNAFSYDFKPWDGIVAGTFDFTGLASHEIGHALGFVSGVDILD
                     YYAGHGGPYGEDNFLLDPLDLFRYSVDSAALGVADFTADARAKYLSLNDGRTLLDSLS
                     TGVYYGDGRQDSHWKDNLGIGVMDPTAAAGELLKFGEADLRALDVVGWNRISATGSAA
                     SAPGSAGIAGTAAVPANAVDVDAPATLWLLGSAALLLLARRRTAGAG"
     gene            complement(5984..7621)
                     /locus_tag="PK144_05630"
     CDS             complement(5984..7621)
                     /locus_tag="PK144_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNF65241.1"
                     /translation="MTDAAARCPRVSHFREILLWPLQLMPLRADSQIQNHWQVLEQSQ
                     AQHPWREVDDEFTGDPAGFQERHYGEFVTFLPHVRRFLYGDGKGRVSKPAESPIRVFR
                     RNDVKRVRCTFADPQRPVLVLEVAHVDLYFFYDLDVTVLTIELYGSDLDLQVVQDLMF
                     RFGRAYPVYWEADGSGGHCLQRVEWLAADGSVLAASDYEDRGRFLRHVCEYRAPTEAA
                     HWDFILRPLVQHHSDAGGVLRYRQLEYHRMPVMGYLAIDGDPRQLSRNDFMRLTFVTP
                     PGPPERAPYCGAEADGFEARHCYDRFWHTAPDATPGGRYLCCGEAFMMVGSAQSPYFY
                     TPGASLLEQFRHQYFLLFLIPHLHKAALFMMADRLGYWLKQLDIHDPESVKRFKREIR
                     RIKEIFLRFTHRYWFRQLSDQYQARELYRMQREFLGTEALYTEVRERIEDMNIYLDSD
                     SLRRQANTVVRLTVVTAFGLIGTTVTGFFGMNLLGFDGLSPAGKALYFLGVLAATTWV
                     TFYTVMKSKRLSDFLDLLSDEREPFWRKIAALLKVWR"
     gene            7746..10172
                     /locus_tag="PK144_05635"
     CDS             7746..10172
                     /locus_tag="PK144_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNF65242.1"
                     /translation="MPPNLTAAALRRGRAIVLMAIAYFVLARLGLLLAIPPGYATAIW
                     PPSGIALAGVLLYGARVAPGIWLGSFVANAVTAADLSGLPALLHSLLLPAVIGCGAML
                     QALFGAWLIRRWVGFPGPLQREREIIAFLLLGGPVGCLLSASIGASALVLAGVVAAHA
                     WAFTWWNWWVGDSIGVLVVTPLVLVALGRPRRLWRRRALTVAGPLTITFAAAVLVFVW
                     TSGREQARLQAEFDARAAALASALSDNFDDYVGVLYSLRDFYAASQDVESEEFARFAQ
                     PALQRHPGLRALSWNRVLDDGQRDAWEQAMRAGGQPGFTLTERAADGQLQPAARRSAY
                     VAIETIVPDRGNHSAIGYDVASDPVRRAAMERARDSGQPVATSRIALVQDASAARGML
                     VFMPIYANDQPVETIEQRREALRGYGVGVFLIDEIVATAVRGLAPQGIELRIREAGAR
                     LLCSYRDPGPCRDDGLEPAPAARGTLLWTSEREFAGNRWRLDLVSTAEYEAAQRSLVA
                     WAVLAGGLLFTGLLGAFLLAVSGRAAVVERLVEERTRALEAANVALAHEIAERRRYET
                     RLQEQNVELVAASRAKDRFLASMSHELRTPLNAIIGFTGILLMKLSGPLSAEQEQQLR
                     TVRSAADHLLALISDLLDLARIESGAAELSREPVDLAEVLAETQRTLLPLAGERGLVL
                     ALQVPDGDWTIPCNRRAFGQIVLNLAGNAIKFTPAGRIDVRLARIADGSGERIQLSVS
                     DTGPGLDPEAQARLFRPFEQLGTPARQEGTGLGLYLSQKLAHQLGGHISCRSKPGEGC
                     TFTLVLPTGE"
     gene            complement(10353..10721)
                     /locus_tag="PK144_05640"
     CDS             complement(10353..10721)
                     /locus_tag="PK144_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNF65243.1"
                     /translation="MADNFEKFFEQTKTLGAPVVKANKLAVASLEKLVALQLSTLQSY
                     TDLTIARLKAAAEVTDLKSLQAFAADQVELAKSLGQKLMDDAKAYADLGAGVKAEFDK
                     LAEEAAAELPKSVQSFSKAA"
     gene            10946..11854
                     /gene="rdgC"
                     /locus_tag="PK144_05645"
     CDS             10946..11854
                     /gene="rdgC"
                     /locus_tag="PK144_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007151671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNF65244.1"
                     /translation="MWFKSLMLFRFTEPCTLDPDTLAEALAHAAFRPCGSQDLVTCGW
                     VPPLGGEDGALVHAAGGFWLVCLQSEDKLLPASVIRAALDERVAEIEDREARKVRRKE
                     KETLRDEVLLDLLPRAFTRRKRLYAYLDPRGGWLVVNGTPGKAVDDFTSLLRKAAGSL
                     PIAPLRTAAVPASVLTGWLGGAPADWQVGDECELRDAGEEGGIVRCKGQDLGGDEIRT
                     HLAAGKQVTRLAVEWQERLSCVLGDDHAVRRLRFLDVVQEQLADLNAETVAEELDARF
                     ALMTLELAAFLPALVSAFGGEAPAAA"
     gene            11970..13103
                     /locus_tag="PK144_05650"
     CDS             11970..13103
                     /locus_tag="PK144_05650"
                     /inference="COORDINATES: protein motif:HMM:NF023329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HNF65245.1"
                     /translation="MTGRLNPAKLFCTAVLMLVAGLAHAAELRELLYDRSTSPELKAA
                     IAVLRDPATPKVPYAQLERRSDIFVRIAPRKVVIGPDGKLLDSAILGTKPFVFVTTPE
                     SVQGKSLLDIYLDIGYEAEDIIAWQRDQDMVAIVYRFPPAVTVSEVRDGALPQDWQTH
                     VYSPTWANAFVLFDRLAAAATVEPERKGEFAPWQTFFRSEAEKTFVLDFPAVARQRVA
                     GTPYARLAALGGDDWKYRSLLETKLSLFEHFRGTGRTQNELEDRDGSKPDTGVLEFVG
                     PNQKLPSLPELAVVHLGGLTLRDSYLPSGVGVWVAASGGAVPFGAIVGGHEAAPGNEP
                     LYICRATHNGGVHPGKLRAAFKGCNIGWGGRETVVADYEVLVR"
     gene            complement(13117..13743)
                     /locus_tag="PK144_05655"
     CDS             complement(13117..13743)
                     /locus_tag="PK144_05655"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNF65246.1"
                     /translation="MSSVYALKPRFQAWLRPAVARLAAAGITANQVTLAAAGASMLLG
                     VALYAAPNVPALWLLLPLFLFVRMALNAIDGMLAREHGQQSALGACLNELGDVFADAL
                     LYLPFARLPACPTRWVVAFVLLAVICEMAGVLMQTLGASRRYDGPMGKSDRAVACGAL
                     GLALGLGATPGPWLEVAFVIFSALGLWTLVRRVTRGLAERGAAPAGRA"
BASE COUNT         2030 a         4748 c         4801 g         2107 t
ORIGIN      
        1 gacacgtcga gcaccgggcc gagcacgtcg agcacggcgt cgagcttggc gcggtacagc
       61 gcacggttgg cctgcacgtg cgcctcgtcg ccccaggccg cgacgctcgc cgcctgcgtg
      121 ctgccgggca gcgcgcagcc gtgataggtg cggtactgca ggaaggcggc gagcacgtcg
      181 gcatcgccgg cgacgaagcc cgaacgcagg ccgggcaggt tcgagcgctt cgacagcgag
      241 ttgaacacca cgcagcgacg gtagtcgtgg cggccgagcc gggcacaggc ttcgagcagg
      301 ccgaccggcg gcgcggcttc gtcgaagtag atctcggaat aacactcgtc ggcggcgatc
      361 acgaaaccga actcgtcggc cttgtcgatc agcttcgcga gcgtcgcggc cggcgtcacg
      421 gcgccggtcg ggttgccggg tgagcacagg tacagcaact ggcaacgcgc ccagacctcc
      481 tctggcacgg cgtcgtaatc gggcaggaag ccggttccgg ccggtgtgtt caggaagtac
      541 ggctcggccc cggcgagcag cgccgcgcct tcatagatct gatagaacgg attcggcatc
      601 agcaccagcg gccggcccgc ggccgtgtcg ctgcgcgcca ccaccgcctg cgcgaacgcg
      661 aacagcgcct cgcgcgtgcc ggccaccggc agcacctgcc ggtccgcatc gagtcccgtc
      721 agccggtagc ggcgcaccgc ccagtcagcg atcgcggcgc gcagcgccgg cgtgccccgg
      781 gtcagcgggt agttcgacag cgcaccgagg ttcgcaacca gcgcatcgag cgcgagctgc
      841 ggcgccggat gctgcggctc gccgatgtac agcgggatgt gcgcgagccc gtgcggcgcg
      901 tgcaggccgg acttcagccg ggccagcttc tcgaacggat agggttgcag gcggtcgaga
      961 tcgggattca tgcgcgaagt atcggtacgg atcggtccgg caaggggcgc ggagtataag
     1021 ccgcgcgggc gcgggcacca tagccgcggc cggccatggt attgtgcgcg cacgcaacaa
     1081 tccgttgctt tgttgtcgat tatccagact gatacatcat gtcgattgca cgtctgcttt
     1141 ctgctgcggc gctggccgca ctgctgtcgg cctgcatgcc gctgtccccg ctgccggccg
     1201 aagcgcccgc cgatgcaacg gccgtcagcg gccgcttgcc gaaggccgat ctcatcctcg
     1261 tgaagaagtc cgaccgccgg ttgacgctgc tgagaaacgg cagggaaatc aaaagctaca
     1321 aggtttcgct cggccgcaat ccggtcggcc agaaggtcgc tgccggcgac gggcgcacgc
     1381 ccgaaggcga ttacctgatc gactggcgca agccggacag caagttctac aaggcgctgc
     1441 acatctcgta tccgcgcgtc agcgaccggc gcaccgccga ggaagccggc cgcaaccccg
     1501 gtggcgcgat catgatccac ggcctgccga atgaccggcc gcgcgcgcat cactatctgc
     1561 cgggcatcga ctggaccgag ggctgcatcg cggtcaccaa ccgcgagatg gacgagatct
     1621 ggcactcggt gcgcgacggc acgccgatcc gcatcctgcc ctgaggccgc atcccggacc
     1681 ggcggcggct tgacggtccc cggtggccgg tcgaggctga ggcagttctt agactgccgc
     1741 cgcaaccgcc gatgaccgag tccgctgccc ctgataccga tccgcgcgcg cgggtcgacg
     1801 acgaactcga ccgcctgctc gccgccggcg ccatcggtcc gctcgatcgc gagtttgcgc
     1861 gcctgctcca gcgcctcgtt ccgaagccgg cgccacgcgc cggcctgctc gccgccgcgc
     1921 tcgccagccg ccagatcggt ctcggccatg tctgcctgcc gctgaccggc ttcgaaggct
     1981 tccgccgccg cctgctcgcc gaggatgaac agggcccggc gatctgggcc ccggctgcgg
     2041 ccgagtggat cgagcggctg cgccggtggc cggcggtcgg cagtcccggc gagttccggc
     2101 cgctggtgct cgatgcggac ggccggctgt acctgtaccg ctactggcgc tacgagaccg
     2161 ggctggcggc gcggctggtg gagcttgcgc aggcgccggt gcgcgtcgat gagccacggc
     2221 tccggcaggg gctggaccgg ctgtttccgc aggcggccgc ggacgccgcc ggcccggacc
     2281 tgcagcggct ggcctgcgcc aatgcggcgc tctcccggct cgcgctgatc tgcggcggac
     2341 ccggcaccgg caagaccacg acgctcgcga agctgctggc gctgctgctg atgcacgatg
     2401 gcgacagcct gcgcatccgg ctggcggcgc cgaccggcaa ggcggcggcg cgcatgctcg
     2461 aagcgctgcg cggtgcccgc gaccggctgg ccgggcagct cgaacccgcg ctgctcgcgg
     2521 cgctgccgac cgatgcctcg acgctgcacc ggctgctcgg cgtgcgtccg gattcgacgg
     2581 cgcgctttcg ccacgacgcc ggacatccgc tcgcgctcga cgtgctggtg atcgacgagg
     2641 tgtcgatggt cgatctgccg ctgctcgcca agaccgtcgc cgcgctgccg gcgcacgcgc
     2701 ggctgatcct gctcggtgac caggatcagc tcgcctcggt cgaggccgga gccgtgctcg
     2761 gcgagctcgg cgcgcaggcc ggtgccccgt cgccgcagcg ggccgcgcag ctcgcggcga
     2821 tcagcggctg caccatcgag gccgatcccg aaccgctgct gccgccgctg cgcgactgcg
     2881 tggcgctgct gcggcgcagc taccgcttcg ccggcgccag cggcatcggg gcgctggcgc
     2941 gcgccgtcaa tgccggcgat gccggggccg cgctcgcgca gctcgaagcc ggcgccgaag
     3001 acctgcagtg gtcgcaggcg acggcgatcg aggcggtgat cgagcgggcg cgcagcggtt
     3061 atcgaagcta tctgcagctg ctcggcaccg ccgcggcggc gcaggacccg caggcactgc
     3121 acgcagcctt cgcgcgcttt cgcgtgctcg gtggcctgcg cagcggggca ctcggggtcg
     3181 agacgctgaa cgcgcggctg gaacgcgagg cgcgccgggc cgccggccgg ccggacacgg
     3241 cgctgtggta tccgggccgg ccggtgatgg tgctgcgcaa cgactacggc ctgaacctct
     3301 acaacggcga tatcggcatc gccaccgtcg acgccgaagg cacgctcgcc gtgcagttcc
     3361 cgcggcccga gggcggcttc cgcgcactgc cggtcgcgcg cctgcccgag cacgagccgg
     3421 tgttcgcgat gaccgtgcac aagtcgcagg gctcggagta cgacgaggtg ctgctggtcg
     3481 cgccgccggc cgactcgccg ctggcgacgc gcgaactgct gtacacgggc ctgacgcgcg
     3541 cgcgcgcgca gctcggcatc gtcgcatccg cagaggcggt ccaggtcgcc tgcagccggc
     3601 gcgtcgcgcg ggactcgggg cttgccgggc gtctggccga ggctgcggcc ggcacgctgg
     3661 tgcagccccc gggcgtgtcg ccgcgcacgc ccggctgatt ccggatggca agcgccggag
     3721 gaggcgccgt accggcgcgc gctgccttcg gcggtgagcg gcaggcgccg tgtnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatgacc gggcgcggtt gccggcgggg
     3901 atcccatcgc gactgcctga ccggcgtcag ggcggaggtc gatgtcggct tgcatgatgg
     3961 atcataagcc agaccaattc tttttctggt cataccaact taccagttgg cgacgcagca
     4021 acccccgact aagcttcgcc cggctgtttt caccatccga taagaggaga gccaacatgg
     4081 cctacagcat cggcaccatc ctgtacgcaa ccgacctcgg cccgcgcagc ctcgaagtct
     4141 tccagcacgc cgtcggctat gcccgccagt tcggcgcgaa gatgcacgtg atcacctgca
     4201 tcgaggcgat cggcgactac gccaacgtca cgctcgacac ctacgtgccg gccgaagtca
     4261 tcgcgcagat ccgcgaggac ggcatcaagc gcatcaacga ggagatcgag acccgcttca
     4321 accgtcacga cgccgcgcac ccggacaaca cctgccgcga ggtcatcgcc tcggtgcaga
     4381 tcatcgaagg cagcgctgcc gacgtcgtgc tgagcgagca gaagcgtctg aatgccgacc
     4441 tgatcgtgct cggctcgcac ggtcacaccg cgcttggcga gatgctgatc ggctcggtcg
     4501 cgcacaaggt caccgtgaag tcgccggtgc cggtgctgct ggtgccgatc aatcagtgag
     4561 cctgcagtga gcctgccggc accgggccgg cctgctccat gtccggaccc cgcgcggctg
     4621 caaggccgcg cggggttttt catgtccggt ttcatgtccg gcttgcaggt gatgcgctgc
     4681 acaagacatg aacgattgta aagaatttac aaggcactga tgggtttgat gtttctgcaa
     4741 taattgcccg aatctgcagg gtcgctcatc cctgcatccc gccggcatcg ccggtgtccg
     4801 gtccgattgc agtgccttcc ctccggcagg tgtcccatgt cttccccgtt ttccgtgcgc
     4861 tgcgcacgat ccggccttgt catcgccacg ctgttgctgc cggtgctgcc ggtacaggcg
     4921 ctgacgttca acttcatcgc cgatgccggc atggatgcgc aggcctacga tgccttcgtg
     4981 cgtgccggca accgctggag cagcgtcttc accgatccgg tcacggtcaa tctgcgcatc
     5041 ggctattcgg cgctcgccag tggcgtgctg gcccaggcct cctcgacgca gacgatcgtg
     5101 ccctacacga atttcgtcag cgccctgcgg gccgatcgca ccagcagcca ggacacgacg
     5161 gcagtcagca atctgtcggc cgacctgaaa ttcctgacca accgtaccag tgacagcccg
     5221 aacggtccgg gttcggcgac gccgtatctc gacaatgacg gttccttcaa caaccagtac
     5281 ctgcgcatga cctcggccaa cgccaaggcc ctgggactga tgtcggcgac cgcgacgggc
     5341 accgatgcga cgatcacgtt cagcaacgcg ttcagttacg acttcaagcc ctgggacggc
     5401 atcgtggccg ggactttcga tttcaccggt ctggcctccc acgagatcgg tcatgcgctc
     5461 ggcttcgtgt cgggcgtcga catcctcgat tattacgccg gccacggcgg gccgtacggc
     5521 gaggacaact tcctgctcga tccgctggac ctgttccgct acagcgtcga cagcgccgcg
     5581 ctcggcgttg ccgatttcac ggccgatgcg cgcgccaagt atctgtccct gaacgacggg
     5641 cgcacgcttc tcgattccct gtcgaccggc gtctattacg gcgacggtcg tcaggacagt
     5701 cactggaagg acaatctcgg catcggcgtc atggatccca ccgccgccgc cggtgaactg
     5761 ctcaaattcg gcgaggcaga cctgcgggcg ctggatgtgg tcggctggaa ccggatttcc
     5821 gcgaccggca gcgcggcttc ggccccgggc agtgccggca tcgccggtac ggcggcggtc
     5881 ccggccaatg ccgtcgatgt cgatgcgccc gccacgctgt ggctgctcgg cagtgcagcc
     5941 ctcctgctgc ttgcccggcg ccggaccgcg ggagcgggct gattcagcgc cagaccttca
     6001 gcagcgcggc gatcttgcgc cagaacggct cgcgctcgtc ggacagcaga tcgagaaagt
     6061 ccgacaggcg cttggatttc atgaccgtgt agaacgtcac ccaggtcgtc gcggcgagca
     6121 cgccgaggaa atacagcgcc ttgccggccg gtgacagtcc gtcgaagccg agcaggttca
     6181 tgccgaagaa cccggtcacc gtcgtgccga tcaggccgaa ggcggtgacc acggtcaggc
     6241 gcacgacggt gttggcctgg cggcgcagcg agtcggaatc gagatagatg ttcatgtcct
     6301 cgatgcgctc gcgcacctcg gtgtagaggg cctcggtgcc gaggaattcg cgctgcatcc
     6361 ggtacagctc gcgcgcctgg tactggtcgg agagctgccg gaaccagtag cggtgcgtga
     6421 agcgcaggaa gatttccttg atcctgcgga tctcgcgctt gaagcgcttg accgattccg
     6481 ggtcgtggat gtcgagctgc ttcagccagt agccgagccg gtccgccatc atgaacagcg
     6541 ccgccttgtg caggtgcggg atcaggaaca gcaggaagta ctgatggcgg aactgctcca
     6601 acaggctcgc gcccggcgtg tagaagtacg gcgactgcgc gctgccgacc atcatgaaag
     6661 cctcgccgca gcagaggtag cgcccgcccg gggtggcgtc cggtgccgtg tgccagaagc
     6721 ggtcgtaaca gtggcgggcc tcgaagccat cggcctcggc cccgcagtac ggcgcgcgtt
     6781 ccggcgggcc gggcggggtc acgaaggtca ggcgcatgaa gtcgttgcgg ctcagctggc
     6841 gcggatcgcc gtcgatcgcc agataaccca tgaccggcat gcggtggtat tcgagctggc
     6901 ggtagcgcag cacgccgcct gcgtccgagt gatgctgcac cagcggccgc aggatgaaat
     6961 cccagtgcgc ggcctcggtc ggtgcgcggt actcgcagac gtggcgcagg aatctgccgc
     7021 ggtcctcgta atcggaggct gcgagcacgc tgccatcggc ggccagccat tcgacgcgct
     7081 gcaggcagtg gccgccgctg ccgtcggcct cccagtagac cggataggcg cggccgaagc
     7141 ggaacatcag gtcctgcacg acctgcaggt cgagatccga gccgtacagc tcgatcgtca
     7201 gcaccgtcac gtcgaggtca tagaagaagt acaggtcgac gtgtgcgacc tccagcacca
     7261 gcaccgggcg ctgcggatcg gcgaaggtgc agcgcacgcg cttcacgtcg ttgcgccgga
     7321 acacgcggat cggggattcc gccggcttcg acacccggcc cttgccgtcg ccgtacagga
     7381 agcggcgcac gtgcggcagg aaggtcacga actcgccgta gtggcgctcc tggaagccgg
     7441 ccggatcgcc ggtgaactcg tcgtcgacct cgcgccacgg atgctgcgcc tgcgactgtt
     7501 cgagcacctg ccagtggttc tggatctggc tgtcggcgcg cagcggcatc agctgcagcg
     7561 gccagagcag gatctcgcgg aaatggctca cgcgcgggca gcgggcagca gcatcggtca
     7621 tggcggacgg cactcgggcg aggggctgcg agtataggca ggcgcagcgc aagggcccgc
     7681 gcgccgggcg tagcggatac catgggtgac tgtgtcattc cgtttccgcg ccggagtccg
     7741 tcctcttgcc cccgaatttg accgctgccg cgctgcgtcg cggccgggcg atcgtgctga
     7801 tggccatcgc ctatttcgtc cttgcccggc tcgggctgct gctggcgatc ccccccggtt
     7861 atgcgacggc gatctggccg ccgtcgggca tcgcgctcgc cggcgtgctg ctctacggcg
     7921 cacgcgttgc gccgggcatc tggctcggat cgttcgtcgc caacgccgtg accgcggccg
     7981 acctctccgg tctgccggcg ctgctgcaca gcctgctgct gccggccgtg atcggctgcg
     8041 gcgcgatgct gcaggccctc ttcggcgcct ggctgatccg gcgctgggtc gggtttccgg
     8101 ggccgctgca gcgcgagcgc gagatcatcg ccttcctgct gctcggcggg ccggtcggct
     8161 gcctgctgtc ggccagcatc ggcgcgagcg cgctggtgct ggccggggtc gtggcggcgc
     8221 acgcctgggc gttcacgtgg tggaactggt gggtcggcga cagcatcggc gtgctggtgg
     8281 tcacgccgct ggtgctggtg gcgctgggcc ggccgcggcg gctgtggcgc cggcgggccc
     8341 tgaccgtggc cgggccgctg accatcacgt tcgcagccgc ggtgctggtg ttcgtgtgga
     8401 ccagcgggcg cgagcaggcg cggctgcagg ccgagttcga tgcgcgggcc gcggcgctcg
     8461 cctcggcgct gtccgacaat ttcgatgatt acgtcggcgt gctgtattcg ctgcgcgatt
     8521 tctacgccgc ctcgcaggat gtcgagtccg aggagttcgc gcggttcgcg cagcctgcgc
     8581 tgcagcgcca tccgggtctg cgggcgctgt cgtggaaccg cgtgctcgat gacgggcagc
     8641 gcgatgcctg ggagcaggcg atgcgcgcag gcggtcagcc cggcttcaca ctgaccgagc
     8701 gcgctgcgga tggtcagctg cagccggcgg cgcgccggtc tgcctacgtc gccatcgaaa
     8761 ccatcgtccc cgatcgcggc aatcacagtg cgatcggtta cgacgtggcc tcggacccgg
     8821 tgcggcgtgc ggcgatggag cgggcgcgcg acagcggcca gccggtcgcc accagccgca
     8881 tcgcgctggt gcaggacgcc agcgccgcgc gcgggatgct ggtgttcatg ccgatctatg
     8941 ccaacgatca gccggtcgag accatcgaac agcggcgcga agcgctgcgc ggctacggtg
     9001 tcggcgtgtt cctgatcgac gagatcgtgg ccacggccgt gcgcgggctg gcgccacagg
     9061 gcatcgaact gcgcatccgc gaggccgggg ccaggctgct gtgcagctac cgtgacccgg
     9121 gaccctgccg ggacgacggg ctggaaccgg cgccggcggc ccgcggcacg ctgctctgga
     9181 cctcggagcg cgaattcgcc ggcaaccgct ggcggctgga tctggtgtcg acagccgagt
     9241 acgaggccgc gcagcgcagc ctggtggcct gggcggtgct cgccggcggg ctgctgttca
     9301 ccggcctgct cggggccttc ctgctcgcgg tcagcggccg tgccgccgtg gtcgagcgcc
     9361 tggtcgagga gcgcacgcgc gcgctggagg cggccaacgt tgcgctcgca cacgagattg
     9421 ccgagcgccg gcgctacgag acacggctgc aggagcagaa cgtcgagctg gtcgccgcca
     9481 gccgcgccaa ggatcgcttc ctcgccagca tgtcgcatga gctgcgcacg ccgctgaacg
     9541 cgatcatcgg cttcaccggc atcctgctga tgaagctgtc cgggccgctc agtgccgagc
     9601 aggagcagca gctgcgcacg gtgcgcagcg ctgccgatca cctgctggcg ctgatcagcg
     9661 acctgctcga tctcgcccgc atcgaatccg gtgccgccga gctgtcgcgc gagccggtcg
     9721 atctggccga ggtgctcgcc gagacgcagc gcacgctgct gccgctcgcc ggcgagcgcg
     9781 gtctggtgct ggcgctgcag gtgccggacg gcgactggac gatcccgtgc aaccgtcgcg
     9841 ccttcggcca gatcgtgctg aacctcgccg gcaacgccat caagttcacg ccggccgggc
     9901 gcatcgacgt gcggctggca cgcatcgccg atggcagtgg cgagcgcatc cagctcagcg
     9961 tcagcgacac cggcccgggc ctcgacccgg aggcgcaggc ccggctgttc cggcccttcg
    10021 aacagctcgg cacgccggcc cgtcaggagg gcaccggcct cggcctgtac ctgagccaga
    10081 agctcgcgca ccagctcggc ggccacatca gctgccgcag caagccgggg gagggctgca
    10141 cgttcacgct ggtgctgccg accggcgagt gaagccggtc acgggtgaat gcgtggtttt
    10201 tctctctctc cccggcaggc actttcccgg catgaggtgt gcggaggcgg gcgaaagaat
    10261 ggtgccgcga aaaagcagaa ccgccgcggc ccctgcgggc ggcggcggtc cggatcggca
    10321 gaccgggcgg cgcgttcagc gccgcggcgg catcaggcag ccttcgagaa cgactgcacc
    10381 gacttcggca gctcggcagc ggcttcttcg gccagcttgt cgaactcggc cttgacgccg
    10441 gcgccgaggt cggcataggc cttggcgtcg tccatcagct tctggcccag gctcttggcg
    10501 agctcgacct gatccgcggc gaaggcctgc aggctcttga ggtcggtgac ttcggccgcg
    10561 gccttcaggc gggcgatggt caggtcggtg tagctctgca gcgtgctcag ctgcagcgcg
    10621 accagcttct ccaggctggc gacggccagc ttgttggcct tgacgaccgg ggcgccgagg
    10681 gtcttggtct gctcgaagaa cttctcgaag ttgtcggcca tggctacttc ctccaaaggg
    10741 gtcaagcgtt gggttgaacg atcatgttgc gaagcagcat atcgacattc atgttgccgt
    10801 gcaacataga tgaccaagat ttcattaagg ttattcggct gaccgtactg cgctgttccg
    10861 gctcggataa atgagccggc gatgacgccg gcagcccgcc tgcttatgat gcgcgccccc
    10921 tgccgccacc cggatgccgt cccgcatgtg gttcaagagc ctgatgctgt ttcgcttcac
    10981 cgaaccctgc acgctcgatc ccgacacgct cgccgaggcg ctcgcgcacg cggccttccg
    11041 gccctgcggt tcgcaggacc tcgtcacctg cggctgggtg ccgccgctcg gcggcgagga
    11101 cggcgcgctg gtgcacgcgg ccggcggctt ctggctcgtg tgcctgcaga gcgaggacaa
    11161 actcctgccg gcctcggtga tccgtgccgc gctcgatgag cgcgttgccg agatcgagga
    11221 ccgcgaggca cgcaaggtcc ggcgcaagga aaaggaaacg ctgcgcgacg aggtcctgct
    11281 cgacctgctg ccgcgcgcgt tcacgcggcg caagcgcctg tacgcctacc tcgatccgcg
    11341 cggcggctgg ctggtggtca acggcacgcc gggcaaggcg gtcgatgact tcacctcgct
    11401 gctgcgcaag gccgccggca gcctgccgat cgcgccgctg cgcaccgccg cggtgccggc
    11461 gagcgtgctg accggctggc tcgggggcgc gccggccgac tggcaggtcg gcgacgaatg
    11521 cgagctgcgc gatgccggcg aggagggcgg catcgtgcgc tgcaagggcc aggacctcgg
    11581 cggtgacgag atccgcacgc atcttgccgc cggcaagcag gtcacgcgcc tggccgtcga
    11641 atggcaggag cggctgtcct gcgtgctcgg tgacgatcac gccgtgcgcc ggctgcgctt
    11701 tctcgatgtc gtgcaggagc agctcgccga tctcaacgcc gagaccgtcg ccgaggaact
    11761 cgacgcgcgc ttcgcgctga tgacgctcga actcgcggcc ttcctgccgg cgctggtcag
    11821 cgctttcggc ggcgaggcgc cggcagcggc ctgagcgccc gcacggccgc gtcgcaagtg
    11881 gcgcttgccg gattgtcgcg gccggtttcc taaggtcgat gcgcgggcat gccgcccgcc
    11941 atccccgacc gcaaggagac gctgacgcca tgaccggccg tctgaacccc gccaagctgt
    12001 tctgtaccgc cgtcctgatg ctggtcgcag gcctcgccca cgccgccgaa ctgcgcgagc
    12061 tgctgtacga caggtccacg agtccggagc tgaaggcggc catcgccgtg ctgcgcgatc
    12121 cggcgacgcc gaaggtgccg tacgcgcagc tcgaacgccg ctccgacatc ttcgtgcgca
    12181 tcgcgccgcg caaggtcgtc atcggccccg acggcaagct gctcgacagc gccatcctcg
    12241 gcaccaagcc cttcgtgttc gtgaccacgc ccgagagcgt gcagggcaag tcgctgctcg
    12301 atatctatct cgacatcggt tacgaggccg aggacatcat cgcctggcag cgcgatcagg
    12361 acatggtcgc gatcgtctac cgcttcccgc cggccgtgac cgtatcggag gtgcgcgacg
    12421 gcgcgctgcc gcaggactgg cagacgcacg tgtactcgcc gacctgggcc aatgccttcg
    12481 tgctgttcga ccggctggcg gcagcggcca cggtcgagcc ggagcgcaag ggcgagttcg
    12541 cgccctggca gaccttcttc cgctccgagg ccgaaaagac cttcgtgctg gacttcccgg
    12601 ccgttgcgcg ccagcgcgtt gccggcacgc cgtatgcgcg cctcgccgcg ctcggcggcg
    12661 atgactggaa gtaccgctcg ctgctcgaga ccaagctcag cctgttcgag cacttccgcg
    12721 gcaccggccg cacgcagaac gaactggagg accgcgacgg cagcaagccc gacaccggcg
    12781 tgctggagtt cgtcggcccg aaccagaagc tgccgtccct gcccgaactc gccgtcgtgc
    12841 acctcggcgg cctgacgctg cgcgacagct acctgccctc gggcgtcggc gtctgggtcg
    12901 cggccagcgg cggagcggtg cctttcggcg ccatcgtcgg cggccacgag gccgcgcccg
    12961 gcaacgaacc gctgtacatc tgccgcgcga cgcataacgg cggcgtccac cccggcaagc
    13021 tgcgcgcggc cttcaagggc tgcaacatcg gctggggcgg cagggagacg gtggtcgccg
    13081 actacgaggt gctggtgcgc taatacgcgt tcacgttcac gcccggcccg cgggcgccgc
    13141 gccacgctcg gccagtccgc gcgtgacgcg gcgcaccagc gtccagagcc cgagcgccga
    13201 gaagatcacg aacgccacct ccagccacgg ccccggcgtc gccccgagtc cgagcgcgag
    13261 cccgagcgca ccgcaggcga cggcacgatc gctcttgccc atcggcccgt cgtagcggcg
    13321 gctggcaccg agcgtctgca tcagcacgcc ggccatctcg cagatcaccg ccagcagcac
    13381 gaaggccacg acccagcgcg tcgggcaggc cggcagccgc gcgaacggca ggtacagcag
    13441 cgcatcggcg aagacatcgc cgagctcgtt caggcaggcc ccgagtgccg actgctggcc
    13501 gtgctcgcgc gccagcatgc cgtcgatcgc gttcagcgcc atgcgcacga acaggaacag
    13561 cggcagcagc agccacagcg ccggcacgtt cggcgccgcg tacagcgcca cgccgagcag
    13621 catcgaggcg ccggcggcgg cgagcgtgac ctggtttgcc gtgatgccgg ccgcggccag
    13681 ccgggccacg gccggtcgca gccaggcctg gaagcggggt ttcagggcat agacgctgct
    13741 catcggggac gatcctgcga gggcggggga gacggccaca gcgtag
//
LOCUS       DAOQIE010000061        13402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2238_length_13402_cov_7.526785, whole genome shotgun sequence.
ACCESSION   DAOQIE010000061 DAOQIE010000000
VERSION     DAOQIE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_13402_cov_7.526785"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="metK"
                     /locus_tag="PK144_05660"
     CDS             complement(<1..790)
                     /gene="metK"
                     /locus_tag="PK144_05660"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNF65247.1"
                     /translation="MSDAYLFTSESVSEGHPDKIADQISDAVLDAIIARDEDARVACE
                     TMVKTGVAIVAGEITTSAWIDLEELVRKVICGIGYTSSDVGFDGATCGVLNIIGKQSV
                     DIAMGVNRKRSEDQGAGDQGLMFGYASTETDVLMPAPICYAHRLVERQAEARKSGLLP
                     WLRPDAKSQVTCRYEDGKVVGIDAVVLSTQHNPEIAHADLREAVMELVIKHVLPAGLI
                     TKDTQFHINPTGNFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGA"
     gene            1026..1589
                     /locus_tag="PK144_05665"
     CDS             1026..1589
                     /locus_tag="PK144_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF65248.1"
                     /translation="MVLLHTPVCDFGLPAPDFDLPGVDGRRWTRDAVRGPAGLLVMFI
                     CNHCPYVQAVRERLVREARDLAALGIGVVAISANDPADYPEDDFAHMQAVAREFGYPF
                     PYLFDESQQVARAYGAVCTPDFFGYNRALELQYRGRLDASGRQPAPAGAVRALYEAMK
                     LVAGTGRGPAAQLPGMGCSIKWKAQGN"
     gene            1829..3823
                     /gene="tkt"
                     /locus_tag="PK144_05670"
     CDS             1829..3823
                     /gene="tkt"
                     /locus_tag="PK144_05670"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005526781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNF65249.1"
                     /translation="MPSRRELANAIRALSMDAVQQANSGHPGAPMGMADIAESLWRGF
                     LRHNPANPHWADRDRFVLSNGHGSMLIYALLHLCGYGLATDDLKAFRQLGSKTPGHPE
                     YGITPGVETTTGPLGQGLANAVGMAIAEKILAAHFNRPGHDLVDHRTWVFVGDGCLME
                     GISHEACSLAGTLGLGKLVAFYDDNGISIDGHVEHWFADDSAQRFAGYGWQVIGPVDG
                     HDGEAVDAAIREALAETARPSLIVCRTVIGYGAPNKQGSHDVHGAPLGAAEIAAARAH
                     LGWPHAPFEVPAEIYAAWDARAAGAGLETAWQAKLEAYRAAFPDLAIEFERRLRGERP
                     AGWAAAADACIEATQARIENLATRVASQKALNAYAPLLPELLGGSADLTPSNNTTWAG
                     SKPVRHADFDGNYLNWGVREFGMSAAMNGIALHGGFIPYGGTFLVFSDYARNALRVAA
                     LAGIPSIFVYTHDSIGLGEDGPTHQPIEQLASLRAMPNLPVWRPCDAVESAVAWRLAI
                     ERRDGPSVLVFSRQNLPHQAREPATVAAIARGGYVLVDCQGTPELMLIATGSEVGLAV
                     AAATELSARGRRVRVVSMPCADVFDAQAPEWREAVLPSACRARLAIEAGATQSWYRYV
                     GLDGRVIGLDRFGESAPAGKLFEHFGFTLANVVTQAETLL"
     gene            3841..4989
                     /locus_tag="PK144_05675"
     CDS             3841..4989
                     /locus_tag="PK144_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011920701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNF65250.1"
                     /translation="MPAGRGRLAINGYGRIGRCVLRALTERGGPAATGLRVVAINEPA
                     ADPASIEYLTRFDSTHGRFPGRVALDGGRLLIDGEPLRLHHAATPGAIDWAAEDIDLL
                     LDCSGSFSTRAAAAPLFAAGAPRVLVSQPMASAAEVDATVVYGINHLALSGRERLVSN
                     ASCTTNCIVPVLAVLDAAFGIESGCITTLHSVMNDQPLIDGWHHADDLRRTRSAMQSM
                     IPVATGLARGIDRLLPGLAGRIVTKAVRVPTLNVSALDTVLMLARDVDAAAVNAVLRA
                     AAEAGPLAGLLAYTELPHASCDFNHDPHSVVVDGSLTHVAGRRLLGLMLWFDNEWGFA
                     QRMLDTAGHWLARAASAAALPDTASASSSHSSPSSRSSATLPPAGNSR"
     gene            4986..6170
                     /locus_tag="PK144_05680"
     CDS             4986..6170
                     /locus_tag="PK144_05680"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNF65251.1"
                     /translation="MKVLKLSDLDLAGKRVFIRADLNVPQDDAGNITDDTRIRASVEG
                     IRLALGKGAAVMVTSHLGRPKEGACTPEDSLAPIAARLGELLNQPVRLVSDWVAGGFT
                     VAPGEVVLLENCRCNKGEKKNDDALAQQMAKLCDVFANDAFGTAHRAEATTHGIARYA
                     PVACAGPLMAAEIEALSRALENPKRPLVAIVAGSKVSTKLTILRSLAEKVDQLIVGGG
                     IGNTFMLASGLRIGKSLAEPDLVGEAQAIIDLMKARGAEVPLPVDVVVADELSALARA
                     NQVAADAVQPGDMILDIGPKSAAQLAYIVAHAGTIVWNGPVGVFEYDQFGGGSKMLAS
                     AIAHSDAFSIAGGGDTLAAIAKYRIADQVGYISTGGGAFLEFLEGKPLPAITVLEARA
                     AG"
     gene            6274..7338
                     /gene="fba"
                     /locus_tag="PK144_05685"
     CDS             6274..7338
                     /gene="fba"
                     /locus_tag="PK144_05685"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNF65252.1"
                     /translation="MALISMRQLLDHAAEHGYGVPAFNVNNLEQMRAIMEAAAQTDSP
                     VIVQASAGARKYAGAPFLRHLILAAIEEFPEIPVCMHQDHGTSPAVCQRSIQLGFSSV
                     MMDGSLGEDGKTPTSYDYNVEVTRQTVAMAHACGVSVEGELGCLGSLETGMAGEEDGI
                     GAEGMLDHSQLLTDPEEAAQFVKATKVDALAIAIGTSHGAYKFTRPPTGDVLAISRIK
                     EIHARIPNTHLVMHGSSSVPQDWLAIINEYGGDLGQTYGVPVSEIVEGIRHGVRKVNI
                     DTDLRMASTGTIRRFLARNPKEFDPRKYLGETVKAMKGICVERYEAFGTAGQASKIRA
                     LSLEAMTKRYASGELDPQVH"
     gene            7414..7986
                     /locus_tag="PK144_05690"
     CDS             7414..7986
                     /locus_tag="PK144_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF65253.1"
                     /translation="MTVVAERRPFTADEFLVWEAQQALRHEYLAGDVFAMTGGTLDHN
                     RLTRNLLMALCRRLDGTPCEAFSSDVMLRVEAADAYFYPDVIVTCSEADRRAERVISE
                     PRVVAEVLSPSTAAHDWGAKFEAYSHLPGLAEYVLVEAERIAVHVLRPNAAQRWERWH
                     CGAGDSLELTSLGLDIPLAELYAGTASAGG"
     gene            8112..8645
                     /locus_tag="PK144_05695"
     CDS             8112..8645
                     /locus_tag="PK144_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF65254.1"
                     /translation="MNAATDTAVRDFFDWQDRLAQRVRPLDEARVHGQLITRLQALLR
                     ERLRGLPARVWAPGPAVHLAEGARTVHPDLAVSSDEADLCSGLPLHAPSLLIEVATAA
                     TAGADRGEGFEQARTLPTLREYVIVEAGRARVDVYRRRADGGWLLDSHGAGEGFVLAA
                     IDGTIEVDALYEGARLR"
     gene            complement(8665..9303)
                     /locus_tag="PK144_05700"
     CDS             complement(8665..9303)
                     /locus_tag="PK144_05700"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNF65255.1"
                     /translation="MSDIEKLIRGFQRFRARYFERDRELFQQLAREGQTPRTLVIACS
                     DSRVDPAIITDAEPGDLFVIRNVANLVPPAEDSGAFHGTSAAVQFAVCDLEVRHIVVM
                     GHGHCGGIRALLEGDAETGYIGNWVKIAGRARAKVLERWPDASPAFRQRAAERMSIIV
                     SLDNLMTFDFVRTRVEAGTLKLFGWYFDIENGEMMQYDPEARRFHDLYLGWD"
     gene            9373..9663
                     /locus_tag="PK144_05705"
     CDS             9373..9663
                     /locus_tag="PK144_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HNF65256.1"
                     /translation="MHCSIYRSRRKTDAYLYCAAKDDFSAVPKALLDQLGQLEHAMDL
                     ELTPERRLAREDAATVLDALATRGWYLQMPQREDWAADWRAAQTPAWGQRVD"
     gene            9750..10940
                     /locus_tag="PK144_05710"
     CDS             9750..10940
                     /locus_tag="PK144_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNF65257.1"
                     /translation="MQFSERLDRLTSSLIRELLALTQQPDMISFAGGLPAAGAMPPLA
                     LANLPPALRQYGTTDGEPELRAAIARQLTGLGRACRPDQVLVTSGSQQGLDLVAKLFI
                     DPGTEVAVEAPSYLAALQAFRFFGAEFAELPLTAHGIDPQQLRETLRRRRPAFVYLIP
                     TFQNPSGVCYDAATRTAVAEVLRETGVALLEDEPYRELVYEPCERTPICAKLGDAAPW
                     MVMGSFSKTGIPGLRIGYIAASDDIHPQLVRIKQCTDLHTNRIGQWWANEFLQSADYL
                     GHLDRLRAYYRAQRDAMQAGLETHFADLARWNRPHGGLFFWVELHEALDERALLERAL
                     ARKVAFMPGSAFYANPPARQGRMRLNFSHSTPEQIDRGLRVLAETVRETSLPAGRRRI
                     ADQV"
     gene            complement(10959..11609)
                     /locus_tag="PK144_05715"
     CDS             complement(10959..11609)
                     /locus_tag="PK144_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNF65258.1"
                     /translation="MTVLLRFLLSACAALSLSVLAPLPARAIPGSPVILVLGDSLSAA
                     YGMPIERGWVALLAARYAAAAPTPPRIVNASISGETTRGGLERLPALLAAHRPDLVVL
                     ELGANDGLRGLPVADLEANLARMIELSQQAGARVLLLGMRIPPNYGPRYTERFHASFA
                     GLAARYDVPLVPFFLDGVAGDPALVQDDGLHPRAEAQPRMLENIWPALDTLLHRPR"
     gene            11611..12333
                     /locus_tag="PK144_05720"
     CDS             11611..12333
                     /locus_tag="PK144_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF65259.1"
                     /translation="MRATPGELDAATGRAVPIVRAERLGKQVASPEGRLTILEDIDLE
                     LAPGEQLAIVGASGSGKSTLLGLLAGLDLPTQGSVWLDGTELSTLDEDGRARLRAGRV
                     GFVFQSFQLLPGLTAIENVMLPLELAGRADAAALAQETLARVGLAARARHYPAQLSGG
                     EQQRVALARAFAPQPAILFADEPTGNLDRRTGETVVGLLFELNRELGTTLVLVTHDAG
                     LAAHCNRQLTLDAGRLVVGAPS"
     gene            12330..>13402
                     /locus_tag="PK144_05725"
     CDS             12330..>13402
                     /locus_tag="PK144_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF65260.1"
                     /translation="MSAAARLLALALRQTWRDARGGELRVLVAALVIACAAVAAVGFF
                     TDRIRLVLERQAGELLAADLVIAGTQPPADFLDAEAGRRGLAHARTLTFPSVVLNADA
                     SALAELKAVAPGYPLRGALKTAPEPYAPAADTRETPVPGTVWADERLLAELGAGVGST
                     LDVGASRLTVTRVLAYEPDRGGDLFSLAPRLMMALADLPATQLVQPGSRVTWRLLLAG
                     TPEAVAGYRDWLGLRLPASMRVQDVREARPELRVALERAGRFLGLAAVAAVLVAGVGI
                     AIAARRYALRHRDAVALMRCFGVTQGRVLALYSIELALLGLAGGLAGAGLGWLAQQGL
                     AAILGELAGGTLPAASWRPLPAAL"
BASE COUNT         1911 a         4854 c         4694 g         1943 t
ORIGIN      
        1 acgcgccgcc gccgtggcgg gccatgccgc cgtaggtgtc gacgatgatc ttgcgtccgg
       61 tcaggccgca gtcgccgacc gggccgccga tcacgaagtt gccggtcggg ttgatgtgga
      121 actgcgtgtc cttggtgatc agcccggccg gcagcacgtg cttgatcacg agttccatga
      181 ccgcctcgcg caggtcggca tgggcgatct cggggttgtg ctgcgtcgag agcacgacgg
      241 cgtcgatgcc gaccaccttg ccgtcctcat agcggcaggt gacctgcgac ttggcatcgg
      301 ggcgcagcca cggcagcagg ccggacttgc gcgcttcggc ctgacgctcg accaggcggt
      361 gcgcgtagca gatcggcgcc ggcatcagca cgtcggtctc ggtgctcgca tagccgaaca
      421 tcaggccctg gtcgccggcg ccctgatcct cggagcgctt gcggttgacg cccatcgcga
      481 tgtcgacgga ctgcttgccg atgatgttca gcacgccgca ggtcgcgccg tcgaagccca
      541 cgtccgagga cgtgtagccg atgccgcaga tgaccttgcg cacgagttcc tcaaggtcga
      601 tccaggcgct ggtcgtgatc tcgccggcga cgatggccac gccggtcttg accatcgtct
      661 cgcaggcgac gcgggcatcc tcgtcgcggg cgatgatcgc atcgagcacg gcatccgaga
      721 tctggtcggc gatcttgtcc ggatggcctt cggacacgga ctcggaggtg aagagatagg
      781 cgtcgctcat gaaacgtcct cgggcggcgg tccgcgcagg atgtgcggcg ggcggggcgg
      841 aaagggtggc cggcagggag ggccgggaac aaaatgcggc ggatcatacc ggccaccgcc
      901 gtcctgtgca tccgtgaccc gctgcagcgc agcgtttcga cacaggacag tacaaatgca
      961 gatggcaaca gggatgtctg tcgctgccgc cgcgtctgcc cgttgcctgc ttccggaggt
     1021 tcccgatggt cctgctgcac accccggtct gtgatttcgg tctgccggcc ccggacttcg
     1081 acctgcccgg cgtcgatggc cggcgctgga cgcgcgacgc cgtgcgcggc ccggccgggc
     1141 tgctggtgat gttcatctgc aaccactgcc cgtacgtgca ggcggtgcgc gagcgtctgg
     1201 tgcgcgaggc gcgcgatctc gccgccctcg gcatcggcgt ggtcgcgatc agcgccaacg
     1261 atccggccga ctaccccgag gacgacttcg cgcacatgca ggcggtcgcg cgcgaattcg
     1321 gttatccgtt cccgtacctg ttcgacgagt cgcagcaggt cgcacgcgcc tacggggcgg
     1381 tctgcacgcc ggacttcttc ggctacaacc gcgcgctcga actgcagtac cgcggccggc
     1441 tcgatgcgag cggccgccag ccggccccgg ccggtgcggt gcgcgcgctg tacgaggcga
     1501 tgaaactggt cgccggcacc ggccgcggcc cggccgccca gttgcccggc atgggctgct
     1561 caatcaagtg gaaggcgcag ggcaactgag gagccggccg gtcgccggcc cggcggtttg
     1621 gcgggccgtg gcgatttccg tatactcgcg cgccacttgc gagccggcgc gcgccgctcg
     1681 cagggctgcg cggcgccata tcccggcgcg gcgcggtccg tgcggcgccc gcgcggcgcg
     1741 cggcggagga ggctgccgcg cagttgcgtt ccggccatct ccgtccgccg acgccgatac
     1801 accccccgcg tccctacgga gacccccgat gccctcgcgc agagaactgg cgaacgcgat
     1861 ccgcgccctg agcatggatg ccgtgcagca ggccaactcc ggccaccccg gcgccccgat
     1921 gggcatggcg gacatcgccg aaagcctctg gcggggcttc ctgcgccaca atccggccaa
     1981 cccgcactgg gccgaccgtg accgcttcgt gctgtcgaac ggccacggct cgatgctgat
     2041 ctacgcgctg ctgcacctct gcggctacgg tctcgcgacc gacgatctca aggccttccg
     2101 ccagctcggc tcgaagacgc cgggtcatcc tgaatacggc atcacgcccg gcgtcgagac
     2161 cacgaccgga ccgctcggtc agggcctcgc caatgccgtc ggcatggcga tcgccgagaa
     2221 gatactggcc gcgcacttca accgccccgg ccacgacctc gtcgatcacc gcacctgggt
     2281 gttcgtcggc gacggctgcc tgatggaagg catctcgcac gaggcctgtt cgctcgccgg
     2341 cacgctcggc ctcggcaagc tggtcgcgtt ctacgacgac aacggcatct cgatcgacgg
     2401 tcacgtcgag cactggtttg ccgatgactc cgcgcagcgc ttcgccggct acggctggca
     2461 ggtcatcggg ccggttgacg gtcacgacgg cgaggccgtc gatgcggcga tccgcgaggc
     2521 cctcgccgag acggcccgcc cgagcctgat cgtctgtcgc accgtcatcg gctacggcgc
     2581 cccgaacaag cagggctcgc acgacgtgca cggcgcaccg ctcggcgccg ccgagatcgc
     2641 cgccgcccgt gcgcacctcg gctggccgca cgcaccgttc gaggtgccgg ccgagatcta
     2701 cgccgcctgg gacgcgcgcg cggccggtgc cgggctggaa accgcctggc aggcgaagct
     2761 cgaagcctac cgcgcggcct tcccggatct ggccatcgag ttcgagcgcc ggctgcgcgg
     2821 cgagcggccg gccggctggg cggcggcggc cgatgcctgc atcgaggcga cgcaggcgag
     2881 gatcgagaac ctcgcgaccc gcgtcgcctc gcagaaggcg ctgaacgcct acgcgccgct
     2941 gctgccggag ctgctcggcg gctcggcgga tctgacgccg tcgaacaaca ccacctgggc
     3001 cggttcgaag ccggtgcgcc acgccgattt cgacggcaac tacctgaact ggggcgtgcg
     3061 cgagttcggc atgtcggcgg cgatgaacgg catcgcgctg cacggcggct tcatccccta
     3121 cggcggcacc ttcctggtgt tctccgacta cgcgcgcaac gcgctgcgcg tcgccgcgct
     3181 cgccggcatc ccgagcatct tcgtctacac gcacgactcc atcggcctcg gcgaggacgg
     3241 cccgacgcat cagccgatcg agcagctcgc gtcgctgcgc gcgatgccga acctgccggt
     3301 ctggcggccc tgcgatgcgg tcgaatcggc ggtcgcctgg cggctggcga tcgagcggcg
     3361 cgacggcccg agcgtgctgg tgttctcgcg ccagaacctg ccgcatcagg cccgcgagcc
     3421 ggcaacggtc gcggcgatcg cgcgcggcgg ctacgtgctg gtcgactgcc agggcacgcc
     3481 ggaactcatg ctgatcgcca ccggctccga ggtcggcctc gcggtcgccg ccgccaccga
     3541 gctttcggcg cgcggccggc gcgtgcgcgt cgtgtcgatg ccctgtgccg acgtgttcga
     3601 tgcgcaggcg cccgaatggc gcgaagccgt gctgccgtcg gcctgccgcg cgcggctggc
     3661 gatcgaggcc ggggccacgc agtcgtggta tcgctatgtc ggcctcgacg ggcgcgtgat
     3721 cggcctcgac cgtttcggcg aatcggctcc ggccggcaag ctcttcgagc acttcggctt
     3781 cacgctcgcc aacgtcgtca cgcaggcgga gacgctgctc tgatcgaccc ggcccgtccg
     3841 ctgccggccg ggcgcggccg gctcgcgatc aacggctacg gccgcatcgg ccgctgcgtg
     3901 ctgcgcgcgc tgaccgagcg cggcggcccg gcggcgaccg gcctgcgcgt ggtcgcgatc
     3961 aacgaaccgg ccgccgaccc ggcgagcatc gaatacctga cgcgcttcga ttcgacgcac
     4021 gggcgctttc cgggccgggt cgcgctcgac ggcgggcggc tgctgatcga cggcgagccg
     4081 ctgcggctgc atcacgccgc cacgcccggg gcgatcgact gggcggccga ggacatcgac
     4141 ctgctgctcg actgctcggg cagcttctcg acccgcgccg ctgccgcgcc gctgttcgcg
     4201 gccggcgcgc cgcgcgtgct ggtctcgcag ccgatggcat cggcggccga ggtcgatgcg
     4261 accgtcgtct acggcatcaa ccatctggcg ctgagcgggc gcgagcggct ggtgtcgaac
     4321 gcctcctgca cgaccaactg catcgtgccg gtgctcgcgg tgctcgatgc ggccttcggc
     4381 atcgaatcgg gctgcatcac gacgctgcat tcggtcatga acgaccagcc gctgatcgac
     4441 ggctggcatc acgccgatga cctgcgccgc acgcgctcgg cgatgcagtc gatgattccg
     4501 gtcgccacgg ggctggcgcg cggcatcgac cggctgctgc cgggcctcgc cgggcgcatc
     4561 gtcaccaagg ccgtgcgcgt gccgacgctg aacgtctcgg ccctcgacac cgtgctgatg
     4621 ctcgcgcgcg atgtcgatgc cgccgccgtc aacgccgtgc tgcgcgccgc ggccgaagcc
     4681 gggccgctcg ccggcctgct cgcctacacc gaactgccgc acgcctcctg cgacttcaac
     4741 cacgatccgc attcggtcgt cgtcgacggc agcctcacgc acgtcgccgg gcggcggctg
     4801 ctcgggctga tgctgtggtt cgacaacgag tggggcttcg cccagcgcat gctcgacacg
     4861 gccggccact ggctcgcacg cgccgcgtcc gccgctgcgc tccccgacac cgcttccgca
     4921 agctcatccc attcatcccc ttcatcccgc tcatccgcaa cgcttccacc tgcagggaac
     4981 tcccgatgaa agtcctcaag ctcagcgacc tcgatctggc cggcaagcgc gtgttcatcc
     5041 gcgccgacct gaacgtgccg caggacgatg ccggcaacat caccgacgac acgcgcatcc
     5101 gcgcctcggt cgaaggcatc cgcctcgcgc tcggcaaggg cgcggcggtg atggtcacct
     5161 cgcacctcgg ccggccgaag gaaggcgcgt gcacgccgga agactcgctg gccccgatcg
     5221 ccgcgcgcct cggcgagctg ctgaaccagc cggtgcggct ggtcagcgac tgggtggcgg
     5281 gcggcttcac ggtcgcgccc ggcgaggtcg tgctgctgga gaactgccgc tgcaacaagg
     5341 gcgagaagaa gaacgacgac gcgctcgcgc agcagatggc gaagctctgc gacgtgttcg
     5401 ccaacgacgc cttcggcacc gcgcaccgcg ccgaggccac gacgcacggc atcgccagat
     5461 acgcaccggt ggcctgcgcc ggtccgctga tggccgccga gatcgaggcg ctgtcgcgcg
     5521 cgctcgaaaa cccgaagcgg ccgctggtcg cgatcgtcgc cggcagcaag gtcagcacca
     5581 agctgaccat cctgcgttcg ctggccgaga aggtcgatca gctgatcgtc ggcggcggca
     5641 tcggcaacac cttcatgctc gcgagcggcc tgcgcatcgg caagtcgctg gccgagccgg
     5701 atctggtcgg cgaggcgcag gcgatcatcg acctgatgaa ggcgcgcggc gccgaggtgc
     5761 cgctgccggt cgacgtggtc gtcgccgatg aactctcggc gctcgcgcgt gccaatcagg
     5821 tggcggccga tgccgtgcag cccggcgaca tgatcctcga catcggcccg aagtcggccg
     5881 cgcagctcgc gtacatcgtc gcgcacgccg gcaccatcgt ctggaacgga cccgtaggcg
     5941 tgttcgagta cgaccagttc ggcggcggct ccaagatgct cgcctcggcg atcgcgcact
     6001 ccgatgcctt ctcgatcgcc ggcggcggcg atacgctggc cgcgatcgcc aagtaccgga
     6061 tcgccgatca ggtcggctac atctcgaccg gcggcggcgc cttcctggag ttcctcgaag
     6121 gcaagccgct gccggcgatc acggtgctcg aagcccgcgc agccggctga ggcgcgccgt
     6181 cactacacaa tggcgccgtc gcgacggcgg cgccggcaaa accaccgcag gatcgaccca
     6241 gcaaggggca ggcccgcccc gggagcacga acgatggccc tgatttcgat gcgtcagctg
     6301 ctggatcacg ccgccgaaca cggctacggc gtcccggcct tcaacgtcaa caacctcgaa
     6361 cagatgcgcg cgatcatgga agccgccgcg cagaccgaca gcccggtgat cgtccaggcc
     6421 tcggccggcg cccgcaagta cgccggcgcg ccgttccttc ggcacctgat cctcgccgcg
     6481 atcgaggaat tcccggagat tccggtctgc atgcatcagg atcacggcac ctcgccggcg
     6541 gtctgccagc gctcgatcca gctcggattt tcctcggtga tgatggacgg ctcgctcggc
     6601 gaggacggca agactccgac cagctacgac tacaacgtcg aggtcacccg ccagaccgtg
     6661 gcgatggcgc acgcctgcgg cgtgtcggtc gagggcgagc tcggctgcct cggctcgctc
     6721 gaaaccggca tggccggcga ggaggacggc atcggtgccg agggcatgct cgatcactcg
     6781 cagctgctca ccgatccgga ggaagccgcg cagttcgtga aggccacgaa ggtcgacgca
     6841 ctggcgatcg cgatcggcac cagccacggc gcctacaagt tcacgcgccc gccgaccggc
     6901 gacgtgctgg cgatctcgcg catcaaggag atccacgcgc gcatcccgaa cacgcatctg
     6961 gtcatgcacg gctcgtcgag cgtgccgcag gactggctcg cgatcatcaa cgagtacggc
     7021 ggcgacctcg gccagaccta cggcgtgccg gtttccgaga tcgtcgaggg catccgtcac
     7081 ggcgtgcgca aggtcaacat cgacaccgac ctgcgcatgg cctcgaccgg cacgatccgc
     7141 cgcttcctcg cccgcaaccc gaaggagttc gacccgcgca agtacctcgg cgagacggtc
     7201 aaggcgatga agggcatctg cgtcgagcgc tacgaagcct tcggcaccgc cggccaggcc
     7261 tcgaagatcc gggcgctgtc gctggaggcg atgacgaagc gctacgccag cggcgagctc
     7321 gatccgcagg tgcactgagg ctgcgacgcc ggtcgcatgc cagcgcgacc ggcgttagca
     7381 tcgcaagccc cgaaccactg cgggaacgcc ccgatgactg ttgtcgccga gcgccggccg
     7441 ttcacggccg atgaattcct cgtctgggaa gcgcagcagg cgctgcgcca cgagtatctg
     7501 gccggcgacg tgttcgcgat gaccgggggc acgctcgatc acaaccggct gacacgcaac
     7561 ctgctgatgg ccttgtgcag acggctggac ggtacgccct gcgaggcgtt ttcgagcgat
     7621 gtgatgctgc gcgtcgaagc agccgatgcg tatttctatc ccgacgtcat cgtcacctgc
     7681 agcgaagccg atcgtcgtgc cgagcgcgtg atctcggaac cgcgagtcgt tgccgaggtg
     7741 ctgtcaccga gcacggcggc ccatgactgg ggcgcgaagt tcgaggctta cagccatctg
     7801 cccggtctcg ccgagtacgt gctggtcgag gccgagcgca tcgccgtgca cgtgctgcgt
     7861 ccgaacgccg cgcagcgctg ggagcgctgg cactgcgggg cgggcgacag cctggaactg
     7921 acgagtctcg gcctcgacat cccgctcgcc gaactctacg ccggcaccgc atcggccggc
     7981 ggctgacacc ggatcgtcat cccgctgctg catcgtcgat cccgcatccc gaaaccagcg
     8041 ccgcgaccgc cggacggtcc gcggtccgtc atcgaccggc ccgagggccg cgacaggagt
     8101 gcaagcccgt catgaacgcc gccaccgaca ccgccgtacg ggacttcttc gactggcagg
     8161 accgcctcgc gcagcgcgtg cggccgctcg acgaggcccg cgtgcatggg cagctgatca
     8221 cgcgcctgca ggcgctgctg cgcgaacggc tgcgcgggct gccggcgcgg gtctgggcgc
     8281 cggggccggc cgtgcatctg gccgaaggcg cgcgcaccgt gcatccggac ctcgcagtca
     8341 gcagcgacga ggccgatctc tgctcgggcc tgccgctgca cgcgccgtcg ctgctgatcg
     8401 aggtcgccac cgccgccacg gccggggccg accgtggcga gggcttcgag caggcgcgca
     8461 cgctgccgac gctgcgcgag tacgtgatcg tcgaggccgg ccgggcgcgc gtcgatgtct
     8521 accgccgtcg cgccgacggc ggctggctgc tcgacagcca cggcgccggc gaaggcttcg
     8581 tgctggcagc gatcgacggc acgatcgagg tcgatgcgct gtacgagggc gcgcggctgc
     8641 gctgaaggag cggcccgggc gggttcagtc ccagccgaga tacaggtcgt ggaaacggcg
     8701 ggcctcgggg tcgtactgca tcatctcgcc gttctcgata tcgaaatacc agccgaacag
     8761 cttcagcgtg ccggcctcga cgcgcgtgcg cacgaagtcg aaggtcatca ggttgtcgag
     8821 cgagacgatg atcgacatgc gctcggcggc acgctggcgg aaggccggcg aggcatccgg
     8881 ccagcgttcg agcaccttgg cgcgcgcgcg gccggcgatc ttcacccagt tgccgatgta
     8941 gccggtctcg gcgtcgcctt cgagcagcgc gcggatgccg ccgcagtggc cgtggcccat
     9001 gaccacgatg tggcgcactt ccaggtcgca gaccgcgaac tgcaccgcgg cgctggtgcc
     9061 atggaaggcg ccggaatcct cggccggcgg cacgaggttg gcgacgttgc ggatcacgaa
     9121 caggtcgccg ggctcggcat cggtgatgat cgccgggtcg acgcgcgaat ccgaacaggc
     9181 aatgaccagc gtgcgcggcg tctggccttc gcgcgccagc tgctggaaca gctcgcggtc
     9241 gcgctcgaag tagcgcgcgc gaaagcgctg gaagccccgg atcagctttt cgatgtcgga
     9301 catggacgga ctccgcggtt cggacttcgg gcatcatatc gcccctttcc cggccaccaa
     9361 gcgaccgccg ccatgcactg ctcgatctat cgcagccgcc gcaagaccga cgcctacctg
     9421 tactgcgccg cgaaggatga cttctcggcc gtgccgaagg cgctgctcga ccagctcggc
     9481 cagctcgagc acgcgatgga tctggagctg acgcccgagc gcaggctcgc gcgcgaggac
     9541 gcggccaccg tgctcgatgc gctcgccacg cgcggctggt acctgcagat gccgcagcgc
     9601 gaggactggg ccgccgactg gcgtgcggcg cagacgccgg cctggggcca gcgcgtcgac
     9661 tgaagaccgg cccgccgcgg caaggacttg cacgcaccgt acggaaagcg ttagctagaa
     9721 cgcttgtccc gcccgccgcc ggaatcgtca tgcagttctc cgaacgcctc gaccgcctca
     9781 ccagttcgct gatccgcgaa ctgctcgcgc tgacccagca gcccgacatg atctcgttcg
     9841 ccggcggcct gccggcggcc ggcgcgatgc cgccgctcgc gctcgcgaac ctgccgccgg
     9901 cgctgcgcca gtacggcacg accgatggcg aacctgaact gcgtgcggcg atcgcccgcc
     9961 agctcaccgg gctcggtcgc gcctgccggc ccgatcaggt gctggtcacc agcggttcgc
    10021 agcagggcct cgatctggtc gcgaagctgt tcatcgatcc gggcaccgag gtcgccgtcg
    10081 aggcgccgag ctatctggcg gcgctgcagg ccttccgctt cttcggcgcc gagttcgccg
    10141 agctgccgct gaccgcgcac ggcatcgacc cgcagcagct gcgcgagacg ctgcgccggc
    10201 gccggccggc cttcgtgtac ctgatcccga ccttccagaa tccgtccggc gtctgctacg
    10261 acgccgccac gcgcacggcg gtcgccgagg tgctgcgcga gaccggcgtc gcgctgctcg
    10321 aagacgagcc ctaccgcgaa ctggtctacg agccctgcga gcgcacgccg atctgcgcga
    10381 agctcggcga tgcggcgccg tggatggtca tgggctcgtt ctcgaagacc ggcatcccgg
    10441 gcctgcgcat cggctacatc gccgcttccg acgacatcca cccgcagctc gtgcgcatca
    10501 agcagtgcac cgacctgcac accaaccgca tcggccagtg gtgggcgaac gaattcctgc
    10561 agtcggccga ttatctcggc cacctcgacc gcctgcgtgc ctactaccgc gcgcagcgtg
    10621 acgcgatgca ggccgggctc gaaacccatt tcgccgatct cgcgcgctgg aaccggccgc
    10681 acggcgggct gttcttctgg gtcgagctgc acgaggcgct cgatgagcgt gcgctgctcg
    10741 aacgggcgct ggcgcgcaag gtcgcgttca tgcccggcag cgcgttctac gccaacccgc
    10801 cggcccgtca ggggcgcatg cgcctgaact tctcgcactc gacgccggaa cagatcgatc
    10861 gcggcctgcg cgtgctcgcc gagaccgtgc gcgagaccag cctgccggcc gggcgccggc
    10921 gcatcgccga tcaggtctga gcagcgggcg cgccggcttc agcgcggccg gtgcagcagc
    10981 gtgtcgagcg ccggccagat gttttccagc atgcgcggct gcgcctcggc gcgcggatgc
    11041 aggccgtcgt cctgcaccag cgccgggtcg ccggcgacgc cgtcgagaaa gaacggcacc
    11101 agcggcacgt catagcgtgc ggcgagtccg gcaaagctcg cgtggaaccg ttcggtgtag
    11161 cgcgggccat agttgggcgg gatgcgcatg ccgagcagca gcacgcgggc gccggcctgc
    11221 tgcgacagct cgatcatccg cgcgagattc gcctccagat cggcgaccgg cagcccgcgc
    11281 aggccgtcgt tggcgccgag ttccagcacg acgagatccg gccggtgggc ggcgagcagc
    11341 gccggcagcc gttccagtcc gccgcgcgtg gtttcaccgc tgatgctggc gttgacgatc
    11401 cgcggcgggg tcggcgccgc cgctgcatag cgcgccgcca gcagcgcgac ccagccgcgc
    11461 tcgatcggca tgccgtaggc cgcgctcagg ctgtcgccga gcacgaggat caccgggctg
    11521 cccgggatgg cacgggccgg cagcggggcg agcaccgaca ggctcagcgc agcacaggca
    11581 gacaacagga aacgcaggag tacggtcatc gtgcgagcaa cacccggaga actggatgcg
    11641 gcgaccggcc gcgccgtgcc gattgtccgg gccgagcgcc tcggcaagca agtcgcgagc
    11701 ccggagggcc ggctgacgat cctggaggac atcgacctcg aactcgcgcc cggcgagcag
    11761 ctggcgatcg tcggggcttc gggctcgggc aagtcgacgc tgctcggcct gctcgccggc
    11821 ctcgacctgc cgactcaggg ttcggtgtgg ctggacggca ccgagctgag cacgctcgat
    11881 gaggacggcc gcgcgcggct gcgcgccgga cgggtcggct tcgtgttcca gtccttccag
    11941 ctgctgccgg ggctgaccgc gatcgagaac gtcatgctgc cgctggaact ggccgggcgc
    12001 gccgatgccg cggcgctcgc gcaggaaacc ctcgcgcgcg tcgggctggc cgcgcgcgcg
    12061 cggcattacc cggcgcagct ctccggcggc gagcagcagc gcgtggcgct cgcgcgcgcg
    12121 ttcgccccgc agccggcgat cctgttcgcc gacgagccga ccggcaacct cgaccggcgc
    12181 accggcgaga ccgtcgtcgg cctgctgttc gagctgaacc gcgaactcgg cacgacgctg
    12241 gtgctggtca cgcacgatgc cggcctcgcc gcgcactgca accgccagct gacgctcgat
    12301 gccggccggc tggtggtcgg ggcgccgtcg tgagcgccgc cgcccggctg ctggcgctgg
    12361 cgctgcgcca gacctggcgt gatgcgcgcg gcggcgagct gcgcgtgctg gtcgcggcgc
    12421 tggtgatcgc ctgcgcggcg gtggcggccg tcggcttctt caccgaccgc atccggctgg
    12481 tgctcgagcg tcaggccggc gagctgctcg ccgccgatct ggtcatcgcc ggcacgcagc
    12541 cgcccgctga cttcctcgac gccgaggccg gccggcgcgg cctcgcgcat gcgcgcacgc
    12601 tgaccttccc gagcgtggtg ctgaacgccg atgcctcggc gctcgccgag ctgaaggcgg
    12661 tcgcgcccgg ctatccgctg cgcggcgcgc tgaagaccgc gcccgagccg tacgcgccgg
    12721 ccgccgatac gcgcgagacg ccggtccccg gcacggtctg ggccgacgag cgcctgctcg
    12781 ccgaactcgg cgctggcgtc ggcagcacgc tcgatgtcgg cgcgagccgg ctcaccgtca
    12841 cgcgcgtgct cgcctacgaa cccgatcgcg gcggcgacct gttcagcctc gcgccgcggc
    12901 tgatgatggc gctcgccgac ctgccggcca cgcagctcgt gcagccgggc tcgcgcgtga
    12961 cctggcggct gctgctcgcc ggtacgcccg aagcggtcgc cggctaccgc gactggctcg
    13021 gcctgcggct gccagcctcg atgcgcgtgc aggacgtgcg cgaggcgcgt ccggagctgc
    13081 gcgtggcgct ggagcgcgcc gggcgcttcc tcggtctcgc ggccgtcgcg gcggtgctgg
    13141 tcgccggcgt cggcatcgcc atcgccgcgc gccgctacgc gctgcgccac cgcgatgccg
    13201 tggcgctgat gcgctgcttc ggcgtcacgc agggccgggt actggcgctg tattcgatcg
    13261 aactggcgct gctcggcctc gccggcggtc tggccggggc cgggctcggc tggctggcgc
    13321 agcaggggct ggcggcgatc ctcggcgagc tcgccggcgg cacgctgccg gcggcctcgt
    13381 ggcgcccgct gccggcggcg ct
//
LOCUS       DAOQIE010000062        13396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2239_length_13396_cov_6.926617, whole genome shotgun sequence.
ACCESSION   DAOQIE010000062 DAOQIE010000000
VERSION     DAOQIE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_13396_cov_6.926617"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK144_05730"
     CDS             complement(<1..835)
                     /locus_tag="PK144_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNF65261.1"
                     /translation="MSTTPSPAAATLELPVAGMSCAACVRRVERALTAVPGVQQASVN
                     LATGRASLEGQSLDLPGLVAAVERAGFEVPVQTLRLELDGLSCAACVTRVERALARVP
                     GVRSASVNLATAQAEVQALAGVDVQALGAAVSAAGYGARPIGEDAAAHTPPQPPQLLP
                     ALVPLVLSAPLVAPMLLTPFGVHAMLPAWVQAVLAGIVLFGYGQRFYRGAWHALRAGS
                     GTMDTLIALGTTAAYGLSVVLMIRHAGHGMTHLYFEASATVITLVLLGKWLETRARRQ
                     TT"
     gene            1022..1225
                     /locus_tag="PK144_05735"
     CDS             1022..1225
                     /locus_tag="PK144_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNF65262.1"
                     /translation="MNETITLKVPDMNCGHCVRAIETAVRALDARAEVRCDLDAKTVQ
                     VATGLAGAAVLEAIREAGYTPST"
     gene            complement(1207..2988)
                     /locus_tag="PK144_05740"
     CDS             complement(1207..2988)
                     /locus_tag="PK144_05740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65263.1"
                     /translation="MFRTSSESPASGNPGPRADARRRLLRTLLSLLLLWGLVLIAVLQ
                     LRALQGEHADIRLQRISTAVAAETGRRFEAIGTLLGAAQAWATQPEDTRPNAQSPYPL
                     LTSGCRRELGAGCRLLRLDAEGRLQPLHDDSGTILPARPLPLPAPGVALIAHLEAVTA
                     ATGTDLLLYGVAATDSGAAVRAVYAAVPVRDFIDAYATLPPAGEARIELLHADGSPLL
                     EILRADAVAAAGYSRIVALTPLPLRLHVSLPAPPLYQDWRLAAALLIAAAGLYSGRRL
                     LGLRQAAGKPGDGVEPDTLLALRQSQRLGRGGHWVYDARLRTLKLSDELRALLSEALA
                     ANLQPLAAGDDVTPAPGTEQLHSDLQRAIVNECEFAGEYRVSDARDQPQWLSVHADAS
                     YNSEGRYLGHIAYVQDITERKQLELELRRLATTDSLTGILNRGSFLDRTAEEIARARR
                     YGRPLSLIVLDLDDFKTINDRFGHPVGDQCLRAFAETGTACLRRADRLGRLGGEEFAA
                     LLPETGLPAALGAAERIREHVQRLRVPLGDGLDTGFTVSAGVTLLSDHHQSIEQLLAA
                     ADRALYRAKAEGRNRVCLDNDLQVEGV"
     gene            complement(3271..4419)
                     /locus_tag="PK144_05745"
     CDS             complement(3271..4419)
                     /locus_tag="PK144_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNF65264.1"
                     /translation="MNDPASALSAAELQRALGELQRPAAGTAGFATDAEAIAGFLGEF
                     ADVPHTQRSYRKEVVRFHLWCRLQRQRDLAGITRADVQAWEGFLAAPPPEWIGPRHAR
                     LGSPGWRPFEGPLTAASRRQAAVILGGFYGYLQRCRYLDYNPFLVMRRRAGARTDATA
                     PMRVLSRETLERVLQVLGSRADAAGDARRRDEAERRLFTVRFLVNAGLRRAELARARM
                     SDLLQREDGDGRPAQWFLSVCGKGGRLRQVALNEAALQALGRWRSHLQATHGLDAQQG
                     ALLVPWKRAGERAAAGVSEAVVYRLVKAALADAGRVLAAEFPADAALLTRASPHWLRH
                     SYATLTANADVPLELVRDQLGHASLDTTLLYRHREDLERARRLRHVAL"
     gene            complement(4456..5790)
                     /locus_tag="PK144_05750"
     CDS             complement(4456..5790)
                     /locus_tag="PK144_05750"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNF65265.1"
                     /translation="MARTTDTRALTEQAFWELLATARKPTIQAVQEWLLARGLTRRNN
                     NTIGSALEECWQQLGTRLKQERSAPELPEDVVDQVLNLRRRMLEVASSVFDEDRAKLR
                     EEVESIRQQAASGVAEAERGQRELAERLQGSELSAGEARAALAEREARLADLLAELAA
                     VRSDAAAATERAAATAAQVIALEQRAAESDTRYSLELKTAHERYEALQAQMMVDMDRY
                     RTEAAELRQELSADRSQHDGRTDAMTERVVQAENARVAAEARAEGSAAELRKVHGRLV
                     QREMELERLQNEYAQIEGKLDLLAAQLAVAQGRIAELEGRPRGSSPQGNLMPVVTGTG
                     AGKGGGGEPRKRNKPKIPNGNLAGAGAGNPNPQAGGAASPAGRKRKPKPAGNPAGPAS
                     AGNNAQPAAGNRRRKRGGGGGAGGGNAQPLAAGEAGRDAGRDDDDGDNLGNA"
     gene            complement(5926..6333)
                     /locus_tag="PK144_05755"
     CDS             complement(5926..6333)
                     /locus_tag="PK144_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65266.1"
                     /translation="MQRDTVVARYAIALVARERQRQLAVEGWSEAHDDALTGGELGLA
                     AACYAAAPQPLYTGSPEAPVDAWPFAPAQDKRGRHDTVRRLAIAGALLLAELERELRR
                     GGLAEAAAADPDLVNELYAAARAVSRQGRLQDE"
     gene            complement(6384..6821)
                     /locus_tag="PK144_05760"
     CDS             complement(6384..6821)
                     /locus_tag="PK144_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65267.1"
                     /translation="MAGIDPELPARWLDIQRRLWAPWLEATGKPNGGRAAGTADLSGL
                     PAFFEAQARQWLEFQRECARALMQSGGGDPAHIAALLDAQARAAEGLLHFQRQCLDGW
                     LHAAGSFDWMQWLGSLQRLPQAMQTGEATPAPSLAPLYPARRD"
     gene            complement(6953..7153)
                     /locus_tag="PK144_05765"
     CDS             complement(6953..7153)
                     /locus_tag="PK144_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65268.1"
                     /translation="MNIATLDRLAQLQDVERAVIDLLFADRTLETCSRERLAVLLEFI
                     HEARSEALASVHSEAANVVTLR"
     gene            complement(7331..7459)
                     /locus_tag="PK144_05770"
     CDS             complement(7331..7459)
                     /locus_tag="PK144_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65269.1"
                     /translation="MSGLALLAIVAASLALGGLWNHLFLKDIGWRTPRAPHVRPQH"
     gene            7641..7730
                     /gene="kdpF"
                     /locus_tag="PK144_05775"
     CDS             7641..7730
                     /gene="kdpF"
                     /locus_tag="PK144_05775"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNF65270.1"
                     /translation="MSLIHLLAAIVAAALALYLLAALCWPERF"
     gene            7743..9488
                     /gene="kdpA"
                     /locus_tag="PK144_05780"
     CDS             7743..9488
                     /gene="kdpA"
                     /locus_tag="PK144_05780"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNF65271.1"
                     /translation="MTGNGLAQIALYFLVLTGLAVPLSAWMERIYAGRAGWLETVLGP
                     LERGLYRLIGPAAGQAQDWRSYALAVLVFSGAGIVLLFVLQLAQAALPLNPQAQPAVP
                     AALALNTAVSFVTNTNWQNYAGEATLGYAVQMLGLTVQNFLSAATGLAVMLALFRGFT
                     QRESATLGNFWVDLVRSVLYLLLPLALGFALVLVACGVVQSFAPYVQATLLQPLADAD
                     GHTLGSQTIALGPVASQLAIKHLGTNGGGFFNANAAHPFENPTPLTDFLLVLAQTVIP
                     AASVLLFGRLLGDRRQGRALLAGMLLVLALAALAGYAAESAGNPRLAALGVDQAGTDA
                     SPGGNLEGKEVRFGLARSALFAATTTATSTGAVDAMHDSFTPLGGLVPLALMQCSETI
                     LGGVGAGMYGMLVYAIVAVFVAGLMVGRTPEYLGKKIEPFEMKMAAIVVLLMPALVLL
                     PVAAGAIDAGVTATLSNPGAHGFSELLYAYTSMANNNGSAFGGLNGNTPFHNLAGALV
                     MLLGRFGTIVPVLAIAGSLAARKIVPPGAGTLPTHTPLFIGFLLGTVIVVGALTFFPV
                     LALGPIVEHLQLWAQ"
     gene            9502..11580
                     /gene="kdpB"
                     /locus_tag="PK144_05785"
     CDS             9502..11580
                     /gene="kdpB"
                     /locus_tag="PK144_05785"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNF65272.1"
                     /translation="MTRIRHSLFDPALSRRAAIDAVRKLDPRHQWRNPVMFTVWAGSL
                     FATGLGLAALAGHAEAPAGFTFAIAAWLWFTVLFANFAEAMAEGRGKAQAESLRRTRT
                     QGQARRLDGTTHGSGFGWIAADRLRAGDVVEVRAGELIPGDGEVIDGIASVNEAAITG
                     ESAPVVRAAGGDHSAVTGGTTVLSDWLVIRIGANPGESFLDRMIGLVEGARRQRTPNE
                     LALAILLAALTLVFLLVTATLLPYAQYAAASGGGQAVSLTVLIALFVCLAPTTIGGLL
                     SAIGIAGMDRMIQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQATEFVPAPGIA
                     PDRLADAAQLASLADETPEGRSIVVLAKERHGLRGRDIHGLDAQFVPFTAQTRMSGVD
                     LPGRAIRKGAVEAIAAWLAQQGRELPAAVREAAEAVSRQGATPLIVAERRATAAGEVL
                     GVVRLKDIVKGGIRERFAQLRRMGIRTVMITGDNALTAAAIAAEAGVDDFLAEARPED
                     KLALIRRLQGEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSNPT
                     KLIEIVEIGKQLLMTRGALTTFSLANDVAKYFAILPAAFAVTYPALGTLNVMGLATPQ
                     SALLSAVIFNALVIVALIPLALRGVRYRPGSAAQLLRSNLLIYGLGGLAVPFAGIKLI
                     DLALSALGLA"
     gene            11632..12219
                     /gene="kdpC"
                     /locus_tag="PK144_05790"
     CDS             11632..12219
                     /gene="kdpC"
                     /locus_tag="PK144_05790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNF65273.1"
                     /translation="MHPLKQQFRPALVALLALTALTGLVYPLVVTGLAQALFPHQAGG
                     SLIVEDGQVRGSALIGQPFADDPRWFWSRPSASAPFPDNAAASGGSNLGPTNPALVAA
                     VQARIDALRAADPGNTAPVPVDLVTASASGLDPHLSPAAAAWQAARVARARGLAPQRV
                     QALIARFTEDRSLGVLGEARVNVLQLNLALARERP"
     gene            12314..>13396
                     /locus_tag="PK144_05795"
     CDS             12314..>13396
                     /locus_tag="PK144_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961446.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HNF65274.1"
                     /translation="MSGRPDPDALLALAQHEERRTRGGRLKVFLGAAPGVGKTWAMLE
                     AAHAQREAGIDVVAGLVECHGRSETEALLDGLELLPRQTSAWHGVHLEEFDLDAALAR
                     RPALLLVDELAHSNAPGARHAKRWQDVQELLARGIDVYTTLNIQHLESLNDVVAGITG
                     IIVRETVPDAVLEQADAVELVDLPPAELLARLRAGKVYLPAQAERAREHFFREANLAA
                     LRELALRQTAAQVNAQVLTLKAGTAPAATWQTAERILACVGPGDGARTVRAARRLAGQ
                     MHAEWTAVHVETPAIARRPAAERAGTIATLRLAEQLGGETLTLAGRAIADEILSHARA
                     RNVTRIVVGRCRRRGWRARLVPGLVDA"
BASE COUNT         1758 a         4705 c         4980 g         1953 t
ORIGIN      
        1 cggtggtctg gcgccgggcg cgggtctcca gccacttgcc cagcagcacc agcgtgatga
       61 cggtcgccga ggcctcgaag tacagatggg tcatgccgtg gccggcgtgc cggatcatca
      121 gcactaccga caggccgtag gcggccgtcg tgccgagcgc gatcagcgtg tccatcgtgc
      181 cgctgccggc gcgcagcgca tgccaggcgc cgcgatagaa gcgctggccg tagccgaaca
      241 gcacgatgcc ggcgagcacg gcctgcaccc aggccggcag catcgcgtgc acgccgaacg
      301 gcgtgagcag catcggtgcg accagcggcg cgctgagcac cagcggcacg agcgccggca
      361 gcagctgcgg cggctgtggc ggggtgtggg ccgcggcatc ctccccgatc ggccgtgcgc
      421 cgtagccggc ggcgctgacc gcggcgccga gggcctgcac atcgacgccg gccagcgcct
      481 gcacctcggc ctgcgcggtc gcgaggttga cgctggccga gcgcacgccg ggcacgcggg
      541 cgagcgcccg ttcgacgcgc gtgacgcagg cggcacagct caggccatcg agttcgagcc
      601 gcagcgtctg caccggcacc tcgaagccgg cgcgctcgac ggctgcgacc aggccgggca
      661 gatcgaggct ttggccttcg aggctggcgc ggccggtggc gaggttgacg ctcgcctgct
      721 gcacgccggg cacggccgtc agcgcccgct cgacgcggcg cacgcaggcc gcacagctca
      781 tgccggcgac cggcagttcc agcgttgccg ccgcggggga gggcgtcgtg ctcatcgtgt
      841 gtctcctcgg ccggaggccg ggccggatga cggaggccgg cagcctcgac cctgacatcg
      901 tggcagggtc aagagggtcg gcagcgcgca ggccggcact tgaccctgac agcctgtcag
      961 ggttcaggct tgcgtatgcc cggcgcaggc ccgggcagac gacgatgcga ggcaggcagc
     1021 gatgaacgag acgatcaccc tgaaggtgcc cgacatgaac tgcggccatt gcgtgcgcgc
     1081 aatcgagacc gccgtgcgcg ccctcgatgc gcgcgccgag gtccgctgcg atctcgatgc
     1141 gaagacggtg caggttgcca cggggcttgc cggggcagcc gtgctcgaag cgatccgcga
     1201 ggccggctac acgccctcga cctgaaggtc gttgtcgagg cagacgcggt tgcggccttc
     1261 ggccttggcg cggtacagcg cccggtcggc cgccgccagc agctgctcga tgctctggtg
     1321 gtgatcggac agcagcgtga cgccggcgct gaccgtgaag ccggtgtcga ggccatcgcc
     1381 gagcggcacg cgcagccgct gcacgtgctc gcggatgcgc tcggcggccc cgagggcggc
     1441 cggcagaccg gtctcgggca gcagtgctgc gaactcctcg ccgccgagcc gcccgaggcg
     1501 gtcggcgcgg cgcaggcagg ccgtgccggt ttcggcgaag gcccgcaggc actggtcacc
     1561 gaccggatgg ccgaagcggt cattgatggt cttgaagtcg tcgagatcga gcacgatcag
     1621 cgacagcggc cgaccgtagc ggcgggcgcg ggcgatctcc tcggcggtgc ggtcgaggaa
     1681 gctgccgcgg ttcaggatgc cggtcaggga atcggtggtc gcgagtcgcc gcagttccag
     1741 ttcgagctgc ttgcgctcgg tgatgtcctg cacgtaggcg atgtggccga gatagcggcc
     1801 ttcgctgttg tagctcgcat cggcatgcac ggacagccac tgcggctggt cacgggcatc
     1861 gctgacccgg tactcgccgg cgaactcgca ttcgttgacg atcgcgcgct gcaggtccga
     1921 gtgcagctgc tcggtgccgg gcgccggcgt cacgtcgtcc ccggcggcga gcggctgcag
     1981 gtttgcggcg agggcttccg agagcagcgc gcgcagctcg tcggacaact tcagcgtgcg
     2041 caggcgcgcg tcatagaccc agtgtccgcc gcggccgagg cgctgggact ggcgcagcgc
     2101 cagcagcgtg tccggctcga cgccgtcacc gggttttccg gccgcttgcc gcagcccgag
     2161 cagccgccga ccgctgtaga gcccggcggc cgcgatcagc agggcggctg ccaggcgcca
     2221 gtcctggtaa aggggcggag ccggcaggct gacgtgcagg cgcagcggca gcggcgtcag
     2281 cgcgacgatc cggctgtagc cggcagccgc cacggcatcg gcccgcagga tttcgagcag
     2341 cgggctgccg tcggcatgca gcagttcgat gcgcgcctcg ccggcgggcg gcagggtcgc
     2401 ataggcatcg atgaaatcgc gcaccggcac ggcggcatac acggcccgca cggccgcgcc
     2461 gctgtcggtg gccgcgacgc cgtagagcag cagatcggtg ccggtggcgg cggtgacggc
     2521 ctccaggtgt gcgatcagcg ccacgccggg ggccggcagc ggcagcggcc gggcaggcag
     2581 gatcgtgccg ctgtcgtcgt gcagcggctg cagacggccc tctgcatcga gccgcagcag
     2641 ccggcagccg gcgccgagtt cgcgccggca gccgctggtc agcagcggat agggcgattg
     2701 cgcgttcggg cgggtgtcct cgggctgcgt cgcccaggct tgcgcggcgc cgagcaaggt
     2761 gccgatggct tcgaaacggc ggccggtttc agcggcgacg gcggtgctga tgcgctgcag
     2821 gcggatgtcg gcgtgttcgc cctgcagggc acgcagctgc agcacggcga tgaggaccag
     2881 gccccagagc agcagcagcg acagcagggt gcgcagcagg cgccggcggg catcggcgcg
     2941 cgggccggga ttgcccgagg ccggagactc cgacgaagtc cggaacatga gcagccatct
     3001 cgtcagcggt gccggcatca gcaagatccc tgtgcgtgcg gggtggtgat ccttcggagg
     3061 tcgggccggc aaggcatccg gtgacgcagg ttatcgattt tgcacgggtg gcgcacggcc
     3121 atgtgcaagc atgcttgcca taaaggcaaa cgttaaattt tgtaaggttt atcacgtctt
     3181 gcccgcacag tccatgcgtc gggacagaca cgggacatgg cacggatcag cgcagcggcg
     3241 ggcgattccg ggcgcggagg gggatgcgcc tcagagcgcg acgtggcgca gccgccgggc
     3301 gcgctcgaga tcctcgcggt ggcggtacag cagcgtggtg tcgagcgagg catggccgag
     3361 ctggtcgcgc accagttcca gcggcacgtc ggcgttggcg gtcagggtcg cgtaactgtg
     3421 ccgcagccag tgcggactcg cccgggtcag cagcgcggca tcggccggga attccgccgc
     3481 cagcacccgg cctgcgtcgg cgagcgcggc cttgaccagc cggtagacga cggcctcgct
     3541 taccccggcc gctgcccgct cgccggcgcg tttccagggc acgagcagcg caccctgctg
     3601 cgcatcgagc ccatgcgtgg cctgcagatg actgcgccag cgcccgagcg cctggagcgc
     3661 ggcctcgttg agcgcgacct gccgcagccg cccgcccttg ccgcagacgc tcaggaacca
     3721 ctgcgcgggc cggccgtcgc catcctcgcg ctgcagcagg tcgctcatgc gcgcgcgcgc
     3781 gagttcggcg cggcgcaggc cggcgttgac gaggaagcgc accgtgaaca gccggcgttc
     3841 ggcctcgtcg cggcggcgcg cgtcgccggc agcgtcggcg cggctgccga gcacctgcag
     3901 gacccgttcg agggtctcgc gcgacagcac gcgcatcggc gcggtggcat ccgtgcgggc
     3961 gccggcgcgc cggcgcatga ccaggaaggg gttgtagtcg agatagcggc agcgctgcag
     4021 atagccgtag aagccgccga ggatcaccgc cgcctgccgg cgcgaggccg ccgtcagcgg
     4081 cccttcgaac ggccgccagc ccgggctgcc gagccgggca tggcgcgggc cgatccactc
     4141 gggcggcggt gcggccagaa agccttccca ggcctgcacg tcggcgcgcg tgatgccggc
     4201 caggtcgcgc tgccgctgca gccggcacca gaggtggaag cgcacgactt ccttgcggta
     4261 gctgcgctgc gtgtgcggca catcggcgaa ttcgccgaga aagccggcga tggcttcggc
     4321 atcggtcgcg aaccccgccg tgccggcggc cgggcgctgc agctcgccga gcgcgcgctg
     4381 cagttcggcc gcggacaggg cgcttgccgg gtcgttcatc gcccgcgcgg cgtgcgcagg
     4441 ccggtcgccg gggcctcagg cattgccgag gttgtcgccg tcgtcgtcgt cacgcccggc
     4501 gtcacgcccg gcttcgccgg cggcgagcgg ctgcgcgttg ccgccaccgg caccaccacc
     4561 gccgccgcgc ttgcgccggc ggttgccggc ggcgggctgc gcattgttgc cggcgctggc
     4621 agggcctgcg ggattgccgg cgggcttggg cttgcgcttg cgcccggccg gcgacgcggc
     4681 gccaccggcc tgcgggttcg ggttgcctgc accggcgccg gcgaggttgc cgttggggat
     4741 cttcggcttg ttgcgcttgc gcggctcgcc gccgccgccc ttgccggcac cggtgccggt
     4801 gacgaccggc atcaggttgc cctgcggcga gctgccgcgc ggccggcctt cgagctcggc
     4861 gatgcgcccc tgcgcgacgg cgagctgtgc ggcgagcagg tcgagcttgc cttcgatctg
     4921 cgcgtattcg ttctgcagcc gttcgagttc catctcgcgc tgcaccagcc ggccatgcac
     4981 cttgcgcagc tccgcggcgc tgccttcggc ccgggcctcg gcggcgacgc gggcgttctc
     5041 ggcctggacg acccgttcgg tcatcgcatc ggtgcgcccg tcgtgctggg aacggtcggc
     5101 actcagttcc tgccgcagct cggcggcctc ggtccggtag cgatccatat cgaccatcat
     5161 ctgtgcctgc agggcttcgt agcgttcatg ggcggtcttc agttcgaggc tgtagcgcgt
     5221 gtcggactcg gccgcgcgct gttccagggc aatgacctgc gccgccgtcg cggcggcgcg
     5281 ttcggtggcg gcggcggcat cgctgcgcac cgccgcgagt tcggcgagca gatcggcgag
     5341 tcgcgcctcg cgctcggcga gggcggcccg ggcttcgccg gcgctcagct cgctgccctg
     5401 cagccgctcg gcgagttcac gctggccgcg ctcggcctcg gcgacgccgc ttgcggcctg
     5461 ctgccggatc gactcgactt cctcgcgcag tttcgcgcgg tcctcgtcga acaccgagga
     5521 ggcaacctcc agcatgcgcc ggcgcaggtt cagcacctga tcgacgacat cctcgggcag
     5581 ttccggggcg ctgcgctcct gcttcaggcg cgtgccgagc tgctgccagc attcctccag
     5641 cgccgagccg atcgtgttgt tgttgcgccg ggtcaggcca cgggcgagca gccattcctg
     5701 caccgcctga atggtgggct tgcgcgccgt cgcgagcagt tcccagaacg cctgttcggt
     5761 cagggcacgg gtatcggtcg tgcgggccat ctctgcatac tcgccgggga gccgcggcga
     5821 attttatgca cagttgacgt gattaacgtg atttttaact tggtccgccg gcgaaagcga
     5881 cacgcattgc gaccggaacg acaccgaagc ggccgcaagc cccgttcatt cgtcctgcag
     5941 ccggccctga cggctgaccg cgcgtgccgc cgcgtacagt tcgttgacca gatcgggatc
     6001 ggccgccgcc gcctcggcga ggccgccgcg gcgcagttcg cgttcgagtt cggcgagcag
     6061 cagcgcaccg gcgatcgcga gccggcgcac cgtgtcgtgc cggccgcgct tgtcctgcgc
     6121 gggcgcgaac ggccaggcgt ccacgggggc ttcgggactg ccggtgtaca gcggctgcgg
     6181 cgcggcggcg tagcaggcgg cggcgaggcc gagttcgccg ccggtcagcg cgtcgtcgtg
     6241 ggcctcgctc cagccctcga cggcgagctg gcgctggcgc tcgcgggcga ccagcgcgat
     6301 cgcgtagcgg gcgacgacgg tgtcacgctg catgacggga agctccggcg ccggctgcgc
     6361 tgaggtacgg ttccgggcgg gcttcagtcg cgccgcgccg gatacagcgg ggccagcgac
     6421 ggtgccggcg tggcttcgcc ggtctgcatc gcctgcggca ggcgctgcag cgagccgagc
     6481 cactgcatcc agtcgaagct gccggccgcg tgcagccagc cgtcgaggca ctggcgctgg
     6541 aagtgcagca ggccctcggc cgcacgcgcc tgcgcatcga gcagggccgc gatgtgggcc
     6601 ggatcgccgc cgccggactg catcagcgcg cgcgcgcatt cgcgctggaa ttccagccac
     6661 tggcgtgcct gcgcctcgaa gaaggccggc agcccgctca ggtcggcggt gccggcggcc
     6721 cggccgccgt tgggcttgcc ggtcgcttcg agccacgggg cccacagccg gcgctggatg
     6781 tcgagccagc gtgccggcag ttccggatcg attcccgcca tcgtcgtctc tcctctgcgt
     6841 ggttcttctt cgggatccgc acgtccggcc gcagccgtcc gccaagggat accgcgcagc
     6901 atagtcgagc ccggtgaccg gggttggcgg tgcgaatgcc ggggcagccg cctcagcgca
     6961 gggtcacgac gttggccgct tcgctgtgca cggaagcgag cgcttccgag cgcgcctcat
     7021 gaatgaattc gagcagcacg gcgaggcgtt cgcggctgca ggtttccagc gtgcggtcgg
     7081 cgaacagcag gtcgatgacg gcgcgttcga cgtcctggag ctgggcgagg cgatcgaggg
     7141 tagcaatgtt catggtcatg gtccgcggtc ccggaattgc tgcggggcaa cgggacgcag
     7201 gatacgggcc accgcgcgcg ccgtccgccg cgtgcggtca cggccggtca caccggcgtt
     7261 aagaaacgtc gcagcacgga acttgcggat ggcgcaaaac gaagcgccgg gctgaccttc
     7321 cggtgcaacg tcaatgttgc gggcgcacat gcggtgcacg cggcgtccgc cagccgatgt
     7381 ccttcaggaa cagatggttc cacaggccgc cgagcgcaag gctggcagcg acgatcgcga
     7441 gcagggcgag tccggacatg atgcacctcc gtatggcgcg ggctggcgcg ggatgcgcgt
     7501 accccgccgg gtggcgtatg cgctccagca tcggtgacag gggcgttacg gaaccattgc
     7561 cgcggcggcc ccggcgttaa cggcttcttt acgggccgca cggccagact gcgcgcagcg
     7621 tttccaccgg agtcccgccc atgagcctga tccacctgct cgctgcgatc gtcgccgcgg
     7681 ccctcgcgct gtatctgctc gccgcgctct gctggcccga gcgcttctga aggcgcgccg
     7741 cgatgaccgg caacgggctt gcgcagatcg cgctgtactt cctcgtgctc accgggctcg
     7801 ccgtaccgct gtcggcctgg atggagcgca tctacgccgg ccgggccggc tggctcgaaa
     7861 ccgtgctcgg gccgctcgaa cgcgggctgt accggctgat cggcccggcg gcgggccagg
     7921 cgcaggactg gcgcagctac gcgctcgccg tgctggtgtt ctcgggcgcc ggcatcgtgc
     7981 tgctgttcgt gctgcagctt gcgcaggcgg cgctgccgct gaacccgcag gcgcagccgg
     8041 cggtgccggc ggcgctggcg ctgaacacgg cggtcagctt cgtcaccaat accaactggc
     8101 agaactacgc cggcgaggcg acgctcggct acgcggtgca gatgctaggg ctgacggtgc
     8161 agaacttcct gtcggcggcg accgggctgg cggtgatgct ggccttgttt cgcggcttca
     8221 cgcagcgcga gagcgcgacg ctcggcaact tctgggtcga tctggtgcgc agcgtgctgt
     8281 acctgctgct gccgctggcg ctggggtttg cgctggtact ggtcgcctgc ggcgtcgtgc
     8341 agagctttgc gccctacgtg caggcgacgc tgctgcagcc gctcgccgat gccgatggcc
     8401 acacgctcgg cagccagacg atcgcgctcg ggccggtggc ctcgcagctc gcgatcaagc
     8461 acctcggcac caacggcggc ggcttcttca acgccaatgc cgcgcatccg ttcgagaacc
     8521 cgacgccgct gaccgacttc ctgctggtgc tcgcgcagac cgtgatcccg gcggcgagcg
     8581 tgctgctgtt cggccggctg ctcggcgacc gccgtcaggg ccgggcgctg ctcgccggca
     8641 tgctgctggt gctggcgctg gcggcgctgg ccggctatgc cgccgagagc gccggcaacc
     8701 cgcgcctcgc cgcgctcggg gtcgatcagg ccggcaccga tgccagcccc ggcggcaacc
     8761 tcgaaggcaa ggaagtgcgc ttcggactcg cgcgttcggc gctgttcgcg gcgacgacga
     8821 cggcaacctc gaccggtgcc gtcgatgcga tgcacgacag cttcacgccg ctcggcggcc
     8881 tggtgccgct ggcgctgatg cagtgctccg agaccatcct cggcggcgtc ggtgccggca
     8941 tgtacggcat gctggtctac gcgatcgtcg cggtgttcgt cgccgggctg atggtcgggc
     9001 gcacgcccga gtacctcggc aagaagatcg aacccttcga gatgaagatg gcggcgatcg
     9061 tcgtgctgct gatgccggcg ctggtgctgc tgccggtcgc ggccggcgcg atcgacgccg
     9121 gcgtcacggc gacgctgtcg aatcccggtg cgcacgggtt ttcggagctg ctgtatgcgt
     9181 acacgtcgat ggccaacaac aacggcagcg ccttcggcgg cctgaacggc aacaccccct
     9241 tccacaacct cgccggtgcg ctggtgatgc tgctcggacg cttcggcacg atcgtgccgg
     9301 tgcttgcgat cgccggcagc ctcgcggccc gaaagatcgt gccgcccggt gccggcacgc
     9361 tgccgacgca cacgccgctg ttcatcggct tcctgctcgg cacggtgatc gtcgtcggcg
     9421 cgctgacctt cttcccggtg ctcgcgctcg gaccgatcgt cgagcacctg cagctgtggg
     9481 cgcagtgaga ggctcccgtc catgacccgc atccggcatt ccctgttcga tccggccctg
     9541 tcccggcgcg cggccatcga cgccgtgcgc aagctcgatc cgcgccatca gtggcgcaat
     9601 ccggtgatgt tcacggtctg ggccggcagc ctgtttgcga ccggcctcgg tctcgcggca
     9661 ctcgccggcc acgccgaggc accggccggc ttcacgttcg cgatcgccgc gtggctgtgg
     9721 ttcacggtgc tgttcgcgaa cttcgccgag gcgatggccg agggccgcgg caaggcccag
     9781 gccgagagcc tgcggcgcac gcgcacgcag gggcaggcga ggaggctcga tggcacgacg
     9841 cacggcagcg gcttcggctg gatcgccgcc gatcgcctgc gcgccgggga tgtcgtcgag
     9901 gtaagggccg gcgaactgat ccccggcgac ggcgaggtca tcgacggcat cgcctcggtc
     9961 aacgaagcgg cgatcaccgg cgagagcgcg ccggtcgtgc gcgctgccgg cggcgatcac
    10021 agcgccgtca ccggcggcac gacggtgctg tcggactggc tggtgatccg catcggcgcc
    10081 aaccccggcg agagcttcct cgaccgcatg atcggtctgg tcgagggcgc gcggcgccag
    10141 cgcacgccga acgagctggc gctggcgatc ctgctcgcag cgctgacgct ggtgttcctg
    10201 ctggtcacgg cgacgctgct gccgtatgcg cagtacgcgg ccgcgagcgg cggcgggcag
    10261 gcggtgtcgc tgaccgtgct gatcgcgctg ttcgtgtgtc tggctccgac cacgatcggc
    10321 ggcctgctgt cggcgatcgg catcgccggc atggaccgga tgatccaggc caacgtcatc
    10381 gccacctcgg gccgcgcggt cgaggcggcc ggcgatgtcg atgtgctgct gctcgacaag
    10441 accggcacga tcacgctcgg caaccggcag gcaaccgagt tcgtgccggc tcccggcatc
    10501 gcgcccgatc gccttgccga tgccgcgcag cttgcctcgc tcgccgacga aaccccggaa
    10561 ggccgcagca tcgtcgtgct cgcgaaggaa cggcacggcc tgcgcgggcg cgacatccac
    10621 gggctcgacg cgcagttcgt gccgttcacg gcgcagacgc gcatgagcgg cgttgacctg
    10681 ccggggcgcg cgatccgcaa gggcgcggtc gaggccatcg ccgcgtggct cgcgcagcag
    10741 gggcgcgagc tgccggcggc ggtgcgcgag gccgcagagg cggtatcgcg ccagggtgcg
    10801 acgccgctga tcgtcgccga gcgccgcgcc acggccgccg gcgaggtgct cggcgtcgtg
    10861 cgcctgaagg acatcgtcaa gggcggcatc cgcgagcgct tcgcgcagct tcggcgcatg
    10921 ggcatccgca ccgtgatgat caccggcgac aacgcgctga ccgccgcggc gatcgccgcc
    10981 gaggccgggg tcgatgactt cctcgccgag gcccggcccg aggacaagct cgcgctgatc
    11041 cgccgcctgc agggcgaggg gcggctcgtg gcgatgaccg gcgacggtac caacgacgcg
    11101 ccggcgctgg cgcaggccga cgtggcggtg gcgatgaaca ccggcacgca ggcggcgaag
    11161 gaggccggca acatggtcga tctcgactcc aacccgacca agctcatcga gatcgtcgag
    11221 atcggcaagc agctgctgat gacgcgcggc gcgctgacca ccttcagcct cgccaacgac
    11281 gtggccaagt acttcgcgat cctgccggcg gcgttcgcgg tcacgtatcc ggcgctcggc
    11341 acgctgaacg tcatggggct cgcgacgccg cagagcgcgc tgctgtcggc ggtgatcttc
    11401 aatgcgctgg tgatcgtcgc gctgatcccg ctggcgctgc gcggcgtgcg ctaccggccg
    11461 ggcagcgcgg cgcagctgct gcgcagcaac ctgctgatct acggcctcgg cggcctcgcc
    11521 gtgccctttg ccggcatcaa gctgatcgac ctcgcgctgt cggcgctcgg gcttgcctga
    11581 acaccggcat ccgtgaccgg tcccgccccg ctttcttccg gaatccgcac catgcacccg
    11641 ctgaagcagc agttccgtcc cgccctggtc gcgctgctag ccctcaccgc gctgaccggc
    11701 ctcgtctatc cgctcgtcgt caccggtctg gcgcaggcgc tgtttccgca ccaggccggc
    11761 ggcagcctga tcgtcgagga cggtcaggtg cgcggcagcg cgttgatcgg ccagccgttc
    11821 gcggacgacc cgcgctggtt ctggtcgcgg ccctcggcga gcgcgccgtt cccggacaat
    11881 gccgcggcct cgggcggcag caatctcggc ccgacgaatc cggcgctggt cgcggccgtg
    11941 caggcgcgca tcgacgcgct gcgcgcggcc gatcccggca acacggcacc ggtcccggtc
    12001 gatctggtca cggcttcggc gagcggtctg gacccgcacc tctcgccggc ggcggccgca
    12061 tggcaggcgg cgcgcgtggc gcgtgcccgc gggctggcgc cgcagcgggt gcaggcgctg
    12121 atcgcccgct tcaccgagga tcgcagcctc ggcgtgctcg gcgaggcgcg cgtcaacgtg
    12181 ctgcagctga accttgcgct ggcgcgggaa cggccatgag gccggggcgg cggtgctggc
    12241 catgtttcga cggtgacggt cgtgaacgac ctgaccgttc ccgttcgcgg tcccgtgcag
    12301 ggaaccggcg cgcatgagcg ggcggcccga tcccgatgcc ctgctcgcgc tcgcgcagca
    12361 cgaggagcgg cgcacgcgcg gcggccggct gaaggtgttc ctcggcgccg cgcccggcgt
    12421 cggcaagacc tgggcgatgc tcgaagccgc gcacgcgcag cgcgaggccg gcatcgacgt
    12481 ggtcgccggg ctggtcgaat gccacggccg cagcgagacc gaagccctgc tcgacgggct
    12541 ggagctgctg ccgcgtcaga ccagcgcctg gcacggcgtg catctggagg aattcgacct
    12601 tgatgccgcg ctcgcgcgcc ggccggcgct gctgctcgtc gatgagcttg cgcacagcaa
    12661 cgcgcccggc gcgcgccacg ccaagcgctg gcaggacgtg caggagctgc tggcgcgcgg
    12721 catcgatgtc tacacgacgc tgaacatcca gcacctcgaa agcctcaacg acgtggtcgc
    12781 cggcatcacc ggcatcatcg tgcgcgagac cgtgcccgat gccgtgctcg aacaggccga
    12841 tgcggtcgag ctcgtggatc tgccgccggc cgagctgctg gcgcggctgc gcgccggcaa
    12901 ggtctatctg ccggcgcagg ccgagcgcgc gcgcgagcac ttcttccgcg aggccaacct
    12961 cgcggcgctg cgcgagctgg cgctgcgcca gaccgccgcg caggtcaacg cccaggtgct
    13021 gacgctgaag gccggcacgg caccggcggc gacctggcag accgccgagc gcatcctcgc
    13081 ctgcgtcggg cccggcgacg gcgcgcgcac cgtgcgcgcc gcgcgccggc tggccgggca
    13141 gatgcacgcc gaatggacgg ccgtgcacgt cgagacgccg gcgatcgcgc gccggccggc
    13201 ggccgagcgc gccggcacga tcgcgacgct gcgcctcgcc gagcagctcg gcggcgagac
    13261 gctcacgctc gccggccgcg cgattgccga cgagatcctg agccatgcgc gcgcgcgcaa
    13321 cgtcacgcgc atcgtcgtcg gccggtgccg gcggcgcggc tggcgcgcgc ggctggtgcc
    13381 ggggctggtc gatgcg
//
LOCUS       DAOQIE010000063        13318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2261_length_13318_cov_7.777275, whole genome shotgun sequence.
ACCESSION   DAOQIE010000063 DAOQIE010000000
VERSION     DAOQIE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2261_length_13318_cov_7.777275"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PK144_05800"
     CDS             <1..1170
                     /locus_tag="PK144_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65275.1"
                     /translation="TRRYGGTGLGLTICRRLASLMEGRLDLHSVPGHGSTFRLHVKLG
                     INGSDPAVPAPFVVRVAGGSHRARAALTGALSGAGHAPVAASSPADLVVLLWEPQADL
                     DALLAAATAPRTVVALRRELLPEDVPAALHALSPGWLPLPWLPNELAAALAGSHAAQM
                     SGPARVQPLPAGLRVLLVEDHPVNQMVAEDMLLEAGAEVSLAGDGQEALDLLERLPDD
                     SFDLVLMDVQMPKLDGIEATRRLRQQPRFDRLPIIALTAHALPEESARCRAAGMNDCL
                     NKPIDQQSLIETVRRHALGSGGAPASLPAGQALIDPGLLPVLRELRQQLHEPTTQIAD
                     RARNLLERIDERQLQPKLREALLLTAAFRYVQAARLFDDLLADDKSSGGDSGRAV"
     gene            1322..2137
                     /locus_tag="PK144_05805"
     CDS             1322..2137
                     /locus_tag="PK144_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF65276.1"
                     /translation="MAAERRTCFIAVANHKGGCGKTTTVVHLGAEFARLGLSVLIVDL
                     DPQANASLHVGRKHPSEVPVTCAELLLAEPEKLPLAIHDDTNIEGVALIYGSLALGRT
                     EDQLRERTPRPAEELRSKLAPLDGIYDVVLMDCPPSLKLLTSNALAAATHLIIPVISG
                     SQYGMYGVTDLMHHVGMIRRINPGLALLGALLMRHDERQTVCKLIEGTARAEIGRLLP
                     VRISTSTKVEQAAMANASLHRWDRSAKVSREFHQLAVALAAELQLSVSGEEDE"
     gene            2134..3057
                     /locus_tag="PK144_05810"
     CDS             2134..3057
                     /locus_tag="PK144_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF65277.1"
                     /translation="MSPRDRKAELARRLEENHQRHSSARQELGFDAGRRHLMLPVDAI
                     DPNPFQPRKVFDEDELRQLAGSIAELGQHQPVVVRPAGERWQLVAGERRWRACRLLGK
                     PTIEALVQDADDARMAVTALAENMEREDLSDYEIGIAIRRIEDQFPSKAKLAEALGRT
                     REDLYKYLAFEALPEDLRARLDAQPRLLSRAAAADVKRMLADERALPLLREAWTKLEA
                     GELQQTRLAAFVAAGLGKAEPAPQRKAVERALHRFTREGQHVGTVSRDAAHFVIRLKV
                     DALSGAQEQRLRSFVEALLAESGPDRNESGT"
     gene            3169..3759
                     /locus_tag="PK144_05815"
     CDS             3169..3759
                     /locus_tag="PK144_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HNF65278.1"
                     /translation="MSRTADALAAFDAINAEDPQRIEVAGVPTPRTLVHARSVSQWIE
                     RLHPQASEALLLAARAHHLRRWEHPRSQWPMTREGYLRWRLELKRIHAEVAAGVLGAL
                     GYEPELISRVGALIRRERLKLDPEAQALEDAICLAFLEHDFDHFGPQHDPAKVIAIVR
                     KTWAKMSEHGRSAALTLSLSDYAQSIVAQALAPTQA"
     gene            3756..5354
                     /locus_tag="PK144_05820"
     CDS             3756..5354
                     /locus_tag="PK144_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65279.1"
                     /translation="MSTQEQRLETLPLLLLDLQATGPRPDVDHPFDLAWWLQTSAGEA
                     IESCPIALPALARLPAILRRRGCEPGTAGALSALAARERLAPVLGASAAIVAHYLRFE
                     AAWLRAWFGDELPPLICLHALSRRLHPRLPSHSLAALAGRLGSEPAQRHRATDHVAAT
                     RAIWRAMLAELADHGIRTLPALLAWLSEPSGPASTGAREYGLDRRLRLALPDAPGVYR
                     YLDARGQVLYVGKATSLHKRVNSYFRKRRGDSAAIRELLTRATGIETTVCASAVHAAI
                     LECREIQHLDPPFNTALKQSRRPLYWLDHELGMVATGHDQRYRIGPWSGSWNVELWLG
                     ACEAVFDGDLRRLQGITAARSDETPQPDAVRTVLLAAWQHPPDRRSLLARAIRRHRHD
                     RSTETGPAAEESEGHASEQGRRPEASAGATEHRLETFDPVTCCARALATIASLHIRSR
                     QLLRLHKRHWQWEEGGQRLSIHLTEGESEGEADATSLSRPMTAIDWDLASVLAGELTR
                     LRQNGCRVWRLPPGGIRKMASTAT"
     gene            5945..6856
                     /gene="trfA"
                     /locus_tag="PK144_05825"
     CDS             5945..6856
                     /gene="trfA"
                     /locus_tag="PK144_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HNF65280.1"
                     /translation="MSDPVRKLNQLQQRATQIDNPLQLPLWHEEKRGVPNEVVRSALF
                     NVRNRCVARREFKDEVIAVLSGDICITYSGTELRQDDMDVWMQLLHEARRQPLGQCVE
                     IKPYEFLKDLRWSPTGYYYKKLREHLKRMKFTMLVVNSARLGREIGVSMIRKYECEGR
                     DGEKLERWRIWIEPEVKALFGDVYYTRIDWQQRVKIKGNLAKHLHGVYSSHAQPYPYK
                     VATILEGCGSDMRELRNFRVSLREALEELQRVGFIEHFQIDDNDLVHVRRTQSSTVAT
                     ELPMLELQALATAKPRKGKRVLPDSDQ"
     gene            6885..7916
                     /locus_tag="PK144_05830"
     CDS             6885..7916
                     /locus_tag="PK144_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF65281.1"
                     /translation="MPGLWRIYRQIQQSKSFSASAIFVLLCALLATALYFLIRWAIID
                     AVWSGESKSCHSQAGACWIFIAEKWRFILFGLYPPSEQWRPTVALLMLSGFFWLIRQS
                     GHMKYPAGLLILLLDVWLIAGGGLLPSIPVALWGGLPLTLLLTLGALLGALPLAIALA
                     FARTSRIYVLRAFAMCYIETLRAVPLISVLFMASVMLPLLMPETSAPDKMLRALGALI
                     LFVAAYMAEAIRGGLVAIAAGQREAAAALGFGRRSYAWLIALPQAMRTAIPALTGIAI
                     GTFKDTSLVLVIGLLDLMTTTRAALSDPQWLGHPLEAYGFAMVLYGVMAWGASQTARH
                     LETTLTHHM"
     gene            8259..8735
                     /locus_tag="PK144_05835"
     CDS             8259..8735
                     /locus_tag="PK144_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65282.1"
                     /translation="MMDRISNPKISFWLRWLLAGGLLLAATLGQSLMAQDDEIVVIVS
                     SDVNILSLTPNGLRYIYSLRLRNWPDGSRIRVYALPDDNETHGEFCRHVLGTYPASLR
                     LAWDRVAYTGIASPAIQVATEEAMVAAIASSPQAIGYVHRKAVTPGVKILPITRER"
     gene            8740..9936
                     /locus_tag="PK144_05840"
     CDS             8740..9936
                     /locus_tag="PK144_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65283.1"
                     /translation="MNRMLVSDRPGALALLGLMWFLPVLVASAKDATDAWPGEWTFNG
                     FLSQGYSLSNTNRFNSSNQQGGSFDLREAAVNTRWQVNDRLSMAAQLLYRDVPVANDD
                     GVRLDYGFIDYSQAAVSGNQNYGVTIGRFKNPFGFFNETRDVPFTRPGVLLPQSLYTE
                     RGRPFAMASQGVLAHADFFTDTGTLALKASFGEPIPGGASLEYILFGNDLSGQFDPDK
                     AVTAQVRYDGAGNTEGWSAALSYLYYSYDFSRPRSLGRDAFNFSFRVMAASLQYSTPD
                     WRITSEAGYGQAFDNIDRFSAYLQGAYRLSPTLESFLRYDLMIVDVEDRWGHEYERQG
                     RGPAHNRYAKDLTFGINWDLSPDLTVRAEWHHVEGTGWLSPAEANLRETDRRWNLGML
                     ELSFRF"
     gene            complement(9945..11333)
                     /locus_tag="PK144_05845"
     CDS             complement(9945..11333)
                     /locus_tag="PK144_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF65284.1"
                     /translation="MPRLPPGPGSIRAGDKLPDHPFAAVHHRPDALPALLHAGAADIL
                     PRTASCAHLSCRGCRGDPRIAPAYQRMISTASGARHMHPIVIVGAGIAGYGLARELRR
                     RDAQIDITLIAADRADFYAKPMLSNALARGQTPETLIASTRADMARQLGITIVPHTCV
                     ERIDPDSRTLLSSSGLYHYRSLVLAIGAEPVRLHFWGDASDEVLSINHLDDYRRFRER
                     LPASGHVALLGAGLIGCEFANDLTACGYRVTVIDPAPRPLAGLLPVPASTQLAEALAA
                     TGVQWRCGHRVRAIDRRAGRYRLSTEDGDTLDADLVLSAVGLRPQTALAAGAGLICER
                     GIVVDRQLRTSAADIHALGDCAQIDGMVRPYVMPAVQAARALASTLTGKPTEMSLPPM
                     PVTIKTPACPIVTCPPPPTLTGVWRELTEDGIRILRFESNDAVLAGFVVIGGHSLARQ
                     QALLREMAESGR"
     gene            complement(11290..11607)
                     /locus_tag="PK144_05850"
     CDS             complement(11290..11607)
                     /locus_tag="PK144_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNF65285.1"
                     /translation="MHVRSPTDAEVLARLERVIDPETGISIIELGLVHGIFREEDRLR
                     ICMTMTTPACPAGTTIIEAVHCALAPLLPAGTAMQVELCRSPVWTPEHATPTARARLD
                     QGR"
     gene            11764..11913
                     /locus_tag="PK144_05855"
     CDS             11764..11913
                     /locus_tag="PK144_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65286.1"
                     /translation="MVASLTDRSAEFLFLSMIYQNADAADHPRRRTGFQVFQIIQKMT
                     VLKEN"
     gene            11917..12495
                     /locus_tag="PK144_05860"
     CDS             11917..12495
                     /locus_tag="PK144_05860"
                     /inference="COORDINATES: protein motif:HMM:NF022664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HNF65287.1"
                     /translation="MIRRSHPPAFRLATLLGAMLAGVLWTGPAIADQRIRCESVDGRQ
                     NYCSADTRGGVRLARQLSRSDCYEGRTWGYDRRGVWVSEGCRAEFIVFEPRGRGYPDD
                     DWGGRGDRGRHGGWDDERRGYGRGPREDVRVRCESDRHRYTSCRIDIGNGRVELVRQF
                     SDEDCRYDRSWGYDRRQIWVDGGCRAEFVIIR"
     gene            12495..13157
                     /locus_tag="PK144_05865"
     CDS             12495..13157
                     /locus_tag="PK144_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF65288.1"
                     /translation="MPAEAQNPPAARSCAPVVVLDFETTGMSPGHGDRITEVGAVRIE
                     DGCITARFQSLVRTGVPVPPFIRQLTGISDAMLAGAPPAEEVLAQLVEFIGRDALVAH
                     NASFDRRFLDAELALIGAARIQPVFCTVLLSRRLLPQAPSHSLGALVSWLGLPSDGVH
                     HRALADAEMTAHLWLHLHQVLDQRYGFSQVSLAWLQKLIDRSPRSADTWLRSQTVEIV
                     RA"
BASE COUNT         2383 a         4293 c         4269 g         2373 t
ORIGIN      
        1 acccggcgct acggcggcac cggcctcggg ctcacgatct gccgccggct ggcgagcctg
       61 atggaaggcc ggctggacct gcattcggtg ccgggccacg gcagcacctt ccggctgcac
      121 gtgaagctcg gaatcaacgg cagcgaccct gccgtgccgg cccccttcgt cgtgcgcgtt
      181 gccgggggca gccaccgggc ccgcgcggcg ctgaccggcg ccctgagcgg ggccggtcat
      241 gccccggtcg ctgcgtcgtc gccggccgat ctggtcgtgc tgctgtggga gccgcaggcc
      301 gatctcgacg cgctgctcgc cgcggccacc gcgccgcgca cggtcgtggc cctgcgccgc
      361 gaactgctgc ccgaggacgt gccggccgcg ctgcatgcgc tgtcccccgg ctggctgccg
      421 ctgccgtggc tgcccaacga actcgcggcc gcgctggccg gcagtcatgc cgcgcagatg
      481 tccggcccgg cgcgcgtgca gccgctgccc gccgggctgc gcgtgctgct ggtcgaggac
      541 catccggtca accagatggt ggccgaggac atgctgctgg aggccggagc cgaggtgagc
      601 ctggccggtg acggccagga agcgcttgat ctgctcgaac gcctgcccga tgacagcttc
      661 gatctcgtgc tgatggacgt gcagatgccg aagctcgatg gcatcgaggc cacgcggcgc
      721 ctgcgccagc agccgcgctt cgatcgcctg ccgatcatcg cgctgaccgc ccatgcactg
      781 cccgaggaat ccgcacgctg tcgcgcagcc ggcatgaacg actgcctgaa caagcccatc
      841 gaccagcagt cgctgatcga gaccgtgcgc cggcatgccc tcggcagcgg cggtgccccg
      901 gcctcgctgc ccgccgggca agccctcatc gatcccggcc tgctgccggt gctgcgggaa
      961 ctgcgtcagc agctgcatga gccgacgacc cagattgccg atcgggcccg caacctgctc
     1021 gaacgcatcg acgaacgcca gctgcagccg aagctgcggg aagcgctgct gctcaccgcc
     1081 gcgttccgct acgtgcaggc ggcccgcctg ttcgatgatc tgctggcaga tgacaagtcg
     1141 tccggtggcg actccggccg tgccgtgtag ccgggagcgg ccgccggcgg gtgtagcaag
     1201 ttgctacacc cgtcagcgca cgcaccggcc gggaagcggt ccgcagccca cacggtttgg
     1261 tcacctgaaa tgacgatgct ataagccgcc atcgtgcacg gaatcgagga ggaccgtacc
     1321 cgtggcagct gaacgccgga cctgtttcat cgccgtcgcc aaccacaagg gcggctgcgg
     1381 caagacgacg accgtcgtgc acctgggggc cgaattcgca cgcctcggtc tgtcggtgct
     1441 gatcgtcgat ctcgatccgc aggccaatgc cagcctgcac gtcggacgca agcacccgag
     1501 cgaggtgccc gtcacctgcg ccgaactgct gctcgccgag ccggaaaaac tgccgctggc
     1561 gatccatgac gacaccaaca tcgagggcgt ggcgctgatc tacggctcgc tggcgctcgg
     1621 gcgcaccgag gaccagctgc gcgaacgcac gccccgcccg gccgaggaac tgcgcagcaa
     1681 gctcgcgccg ctcgacggca tctacgacgt ggtgctgatg gactgcccgc ccagcctgaa
     1741 gctgctgacc agcaacgcgc tggccgccgc tacgcatctg atcatcccgg tgatctccgg
     1801 ctcgcagtac ggcatgtacg gcgtcaccga cctgatgcat cacgtcggca tgatccggcg
     1861 catcaacccc gggctggcgc tgctcggtgc gctgctgatg cgtcacgacg aacgccagac
     1921 cgtctgcaag ctgatcgagg gtacggccag ggccgagatc ggccggctgc tgccggtgcg
     1981 catctcgacc agcaccaagg tcgagcaggc ggcgatggcc aatgccagcc tgcaccgctg
     2041 ggaccggagc gcgaaggtct cgcgcgaatt ccatcagctg gccgtggcgc tggccgccga
     2101 actgcagctg agcgtgagcg gggaggagga cgaatgagcc cgcgtgaccg caaggccgag
     2161 ctggcccgcc ggctcgaaga gaaccatcag cgtcacagca gcgcccgtca ggagctcggt
     2221 ttcgacgccg gtcgccgcca cctgatgctg ccggtcgatg cgatcgatcc gaatccgttc
     2281 cagccgcgca aggtgttcga cgaggacgag cttcgccagc tcgccggctc gatcgccgaa
     2341 ctcggccagc accagccggt cgtcgtgcgc ccggcgggcg agcgctggca gctggtcgcc
     2401 ggtgaacgcc gctggcgggc ctgccggctg ctcggcaagc ccaccatcga ggcgctggtg
     2461 caggacgccg acgatgcgcg catggcggtc acggcactgg ccgagaacat ggagcgcgag
     2521 gatctcagcg attacgagat cggcatcgcc atccgccgca tcgaggatca gtttccctcc
     2581 aaggccaagc ttgccgaagc cctgggtcgc acccgcgagg acctctacaa gtatctggcc
     2641 ttcgaggcgc tgcccgagga tctgcgggcc cggctcgatg cacagccccg gctgctgtcg
     2701 cgggcggcgg cggccgacgt gaaacgcatg ctggccgatg aacgcgccct gccgctgctg
     2761 cgcgaggcct ggacgaagct cgaagccggc gagctgcagc agacccggct cgccgccttt
     2821 gtcgccgccg gtctcggcaa ggctgaaccc gccccgcagc gcaaggctgt cgaacgcgcg
     2881 ctgcatcgct tcacgcgcga agggcagcac gtcggcaccg tctcgcggga cgccgcacac
     2941 ttcgtgatcc gcctgaaggt cgacgccctc agcggcgcgc aggaacagcg actgcgaagc
     3001 ttcgtcgaag ccctgctggc ggaatccggc ccggaccgca acgaatcagg cacctgaacc
     3061 cggcctgcgc ggtgtagcaa gttgctacac cgcggcctgc acagggcgga cgggtgagcc
     3121 tgcagcgggt tacgctgcgt ttcccccttc ctgacccgaa ccgccgccat gtcccgaacc
     3181 gccgacgctc tcgctgcctt cgacgccatc aacgccgagg atccgcagcg catcgaagta
     3241 gccggcgtgc cgaccccgcg cacgctggtc cacgcccgca gcgtttcgca atggatcgaa
     3301 cgcctgcatc cgcaggcgag cgaagccctg ctgctcgcag cgcgcgccca tcacctgcgt
     3361 cgctgggaac acccgcgttc gcaatggccg atgacgcgcg aaggctatct gcgctggcgc
     3421 ctggagctga agcgcattca tgccgaggtc gccgccggcg tgctgggcgc gcttggctac
     3481 gagcccgagc tgatcagccg ggtcggcgcc ctgatccggc gcgagcggct gaagctcgac
     3541 ccggaagcgc aggcgctcga ggacgccatc tgcctggcct tcctcgaaca cgacttcgat
     3601 cacttcggtc cgcagcacga tccggccaag gtcatcgcca tcgtgcgcaa gacctgggcg
     3661 aagatgtccg aacacggccg ctcggctgcg ctgacgctgt ccctgtcgga ctacgcgcaa
     3721 tcgatcgtcg cgcaggcgct ggcaccaacg caggcatgag cacgcaggaa cagcggctcg
     3781 aaaccctgcc gctgctgctg ctcgatctgc aggcgaccgg tccccgcccg gacgtcgatc
     3841 atcccttcga tcttgcctgg tggctgcaga cttcggccgg agaggccatc gagtcctgcc
     3901 cgatcgcgct gccagccctg gcgcgcctgc ccgcgatcct gcgccggcgc ggctgtgaac
     3961 ccggcacggc gggggctttg tcagcgctcg ccgcccgcga acggctggcg ccggtgctgg
     4021 gagcctcggc tgcgatcgtc gcgcattacc tgcgcttcga agcggcctgg ctgcgtgcct
     4081 ggttcggtga cgagctgccg ccgctgatct gtctgcacgc cctgtccaga cgcctgcatc
     4141 cgcgcctgcc cagccattcg ctcgctgccc tggcaggccg cctgggcagc gaaccggcac
     4201 agcgccatcg cgccaccgat cacgttgccg ccacgcgggc gatctggcgc gccatgcttg
     4261 ccgaactcgc agaccacggc atccggaccc tgccggcgct gctggcctgg ctgagcgaac
     4321 cgagcggacc agccagcacc ggagcacgcg aatacggcct cgatcgccgg ctgcgactgg
     4381 ccctgccgga tgccccgggg gtctatcgct atctcgatgc ccgcgggcag gtgctgtacg
     4441 tcggcaaggc cacatccctg cacaagcgcg tgaacagcta tttccgcaag cgccgggggg
     4501 atagcgccgc aatccgcgaa ctgctgaccc gtgcgaccgg gatcgaaacc acggtctgcg
     4561 cgagcgccgt gcatgccgcg atcctcgaat gccgcgaaat ccagcatctc gacccgccgt
     4621 tcaacaccgc gctgaagcaa tcccggcgtc cgctgtactg gctcgatcac gagctgggta
     4681 tggtcgcgac cgggcacgac cagcgatacc ggatcggtcc gtggagcggg agctggaatg
     4741 tcgaactctg gctcggcgcc tgcgaggccg tgttcgacgg cgaccttcgc aggctgcaag
     4801 ggatcaccgc tgcgcggagc gatgaaaccc cgcaaccgga cgccgtgcga accgtgctgc
     4861 tggcggcgtg gcagcatccg ccagaccggc gcagcctgct cgcgcgcgcc atccgacgcc
     4921 atcgtcacga tcggtccacc gaaaccggtc cggctgcgga agaaagcgaa gggcatgcat
     4981 cagagcaagg ccgcagacca gaggcatccg ccggagcgac cgaacaccgc ctggagacct
     5041 tcgatccagt cacctgctgt gcccgggcac tggccaccat cgccagcctg cacattcggt
     5101 cccgacagtt gctgcgcctg cacaaacgcc actggcaatg ggaggagggc gggcagcgcc
     5161 tgtcgattca cctgaccgag ggtgagagcg agggcgaagc ggatgcgacc tccctcagcc
     5221 ggccgatgac agccatcgac tgggacctgg cttccgtgct cgccggcgaa ctcacgcgac
     5281 tgcgccagaa cggctgccgg gtatggcgtc tgccgcccgg cgggattcgc aagatggcca
     5341 gcactgccac gtaacaaggc cagcagggca cggaacatga ccagcagaca cgtcagatgg
     5401 ccagcaaatc ctcgtcagat gaccagcagg cacgcgaaac ttgaccagcg ctttttcgta
     5461 acaaggccag cagacatcgg taaatattca tctgcaatcg actgattcaa ttggattatt
     5521 caggaatgac gcatcaatgc taaccgtctt ttaaccgatc tttaaccaat ctttaaccgc
     5581 agattcgcga acgacaacga atcaggcttg ttttattctt taaaatcaaa cgcttacatt
     5641 attaacatcc atcttcacaa gactcacgga tatctggatc acgtaagaag gccagcgaac
     5701 aagtcataac ctgcaagccg aagaatcgtg gaccggctcc aaagttaacc accacgacgc
     5761 gagcgcatgc ggccttcata gctaagatca gggtctgaca gtcgatcgat ggtcttcaaa
     5821 ccagcatgct ggtcatattt cgtgccctgc tggccatctt tcgtccctgc tggtcttgtt
     5881 acgttactga cccacgacaa gctcttgcct atgctgcaag gtatcgcgag atggagtcac
     5941 cgacgtgtcc gatcctgtcc gtaagctcaa tcagttgcag caacgtgcga cacagatcga
     6001 caacccgctg caactgccgc tctggcatga ggaaaagcgt ggcgtcccga acgaagtcgt
     6061 gcgctcggca ctgttcaacg tccgtaaccg ctgtgtggca cgtcgcgaat tcaaggatga
     6121 agtcatcgcg gtgctgagtg gcgacatctg catcacctac tcgggtaccg agctgcgtca
     6181 ggacgacatg gacgtctgga tgcaactgct gcacgaggcc cgccgccagc ctctcggtca
     6241 atgcgtcgaa atcaagccct atgaattcct gaaggatttg cgctggtcac caaccggtta
     6301 ttattacaag aaacttcggg aacacctgaa acgcatgaag ttcacaatgc tggtagtcaa
     6361 ctctgccaga ctgggacgtg aaatcggtgt ttcaatgatt cgcaaatacg aatgtgaagg
     6421 tcgggacgga gaaaaactcg aacgctggcg catctggata gaaccggaag tcaaggcatt
     6481 gttcggcgat gtctattata cccgcatcga ctggcagcaa cgggtcaaga tcaagggcaa
     6541 tcttgccaaa catctgcacg gcgtatattc cagccacgct caaccttatc catacaaggt
     6601 ggctacgatt cttgaaggtt gtggttcgga catgcgggag ttgcgaaact tccgcgtcag
     6661 tctgcgtgaa gcgctcgaag aattgcagcg ggtaggtttc atcgagcatt tccagattga
     6721 cgacaatgat ctcgtacacg tgcgcaggac acagtcctcc acggtcgcta cggagctgcc
     6781 aatgctcgaa ttgcaggcac tcgcgacggc aaagcctcgc aagggaaaac gcgttctacc
     6841 ggattctgac caatgatcca ttgccttgct gattctcctt gaagatgccg ggtttatggc
     6901 gaatctaccg gcagatccag caatccaagt ctttttctgc cagtgcaata ttcgttctgt
     6961 tgtgtgccct gctggcaacg gcgctgtatt ttctgatccg ctgggcaatc attgatgccg
     7021 tctggtcagg cgaatcaaaa tcctgccatt cacaagctgg ggcctgctgg atattcattg
     7081 ccgaaaaatg gagattcatt ctattcggtc tttatccacc atccgaacaa tggcgaccga
     7141 ctgtcgcgct gctgatgttg tccggctttt tctggctgat acgtcagtca ggccacatga
     7201 aatatccggc gggactgctg atcctgctgc tggatgtctg gctgatcgcc ggtggtggcc
     7261 ttctgccatc cattcctgtg gccctgtggg gtggcttgcc gctgaccttg ctgctcacac
     7321 tgggtgccct gctgggagca ttgcctctgg ccattgcact ggcatttgca cgaaccagcc
     7381 ggatatatgt cctgcgtgca ttcgccatgt gttacatcga aacattgcgg gctgtgccac
     7441 tgatcagcgt gctgttcatg gcatcggtga tgctgccgct gctgatgccg gaaacttctg
     7501 ctcccgacaa gatgctgcgg gccctcggtg ccctgatcct gttcgttgct gcctacatgg
     7561 cggaggccat tcgcggagga ctggtcgcca ttgcggccgg acaacgggaa gctgcagcgg
     7621 ctctgggctt tggcagacgt tcctatgcat ggctgattgc actgccacag gcaatgcgta
     7681 cggcgatacc ggccctgacc ggtatcgcca tcggcacgtt caaggacaca tcgctggtgc
     7741 tggtcatcgg cttgctggat ttgatgacca ctacccgggc tgcgctgagc gatccgcaat
     7801 ggctgggcca cccgctcgaa gcctacgggt ttgccatggt tctctacggg gtgatggcct
     7861 ggggggcgtc gcagactgct cgacacctgg aaacaacgct gactcatcac atgtagcaag
     7921 ttgctacatt catatcctgt cccgccagca gatggaaaaa agcagtacac tgccgggctg
     7981 caccaaccca tgccataaca tcaagggttg cattgcaaat cattgacttg agcgttcata
     8041 tcgatcaggt caccccgatc gcaccagagc tggtgacgct ctgatcgcat cactggcgat
     8101 ccagcgtcag aaaggcaggg aggcattgat gcccggatcc caagcagcat caggcagtgc
     8161 aaggctcgct tccggacttc attccgctgc gtgtgatgct tcctgcccgc atcggggcat
     8221 ccggatgttg tcaaccggca tgattccgtg atcggctgat gatggatcga atctccaatc
     8281 ccaagatcag cttctggctg cgctggctgc tggccggagg tctgctgctg gcagcgactc
     8341 tcggccagtc cctgatggcc caggacgacg agattgtcgt cattgtttcc agtgacgtga
     8401 acatcctgag cctgacaccg aacggactgc gatatatcta ttccctgcgc ctgcgcaact
     8461 ggcccgatgg cagcaggatt cgcgtttatg ccttgcccga tgacaatgag acacatggtg
     8521 aattctgtcg tcatgtactc ggaacctacc cggcttccct gcggcttgcc tgggaccggg
     8581 ttgcctatac cggcattgcc tcgccagcca tccaggtagc cacggaagaa gccatggttg
     8641 cggccatagc cagctcgccg caggccatcg gatatgtaca ccgcaaggcg gtgacgcctg
     8701 gcgtcaagat tctgccaatc acacgggaac ggtgatgcaa tgaacaggat gcttgtctcg
     8761 gaccggcctg gagcgctggc attgctgggg ctgatgtggt tcctgccggt tttggtcgca
     8821 tcagccaagg atgcaacgga cgcatggccg ggagaatgga cgttcaacgg ctttctgagt
     8881 cagggttatt ctctgtccaa taccaatcgt ttcaattcca gcaatcagca gggtggatcc
     8941 ttcgatctgc gggaggctgc ggtcaacacc cgctggcagg tcaatgatcg cctgtccatg
     9001 gcagcgcaac tgctgtatcg ggatgtgccg gtggcgaatg acgacggggt gaggctggat
     9061 tatggcttca ttgactacag tcaggccgcc gtcagcggca atcagaatta cggcgtcacg
     9121 atcggacgtt tcaagaatcc cttcggattc ttcaatgaaa cgcgcgatgt gcctttcacc
     9181 cgcccaggcg tgctgctgcc gcaatccctg tataccgaac ggggacggcc gtttgcgatg
     9241 gcgtcgcagg gagtactggc acatgcggat ttcttcacgg ataccggcac tctggcgctg
     9301 aaggcatcat tcggtgaacc gatacccggc ggtgcatcgc tggaatacat cctgttcggg
     9361 aatgatctca gcggtcagtt cgatccggac aaggcggtga cggctcaggt gcgatacgat
     9421 ggtgctggca acactgaagg ctggagtgct gccctgagct atctgtacta ctcgtatgac
     9481 ttcagtcgac cccgctcgct ggggcgtgat gctttcaact tttccttccg ggtcatggcc
     9541 gcctcactgc agtattcgac gccggactgg cggatcacgt cggaagccgg ctatggacag
     9601 gccttcgaca acatcgatcg cttcagtgcc tatctgcagg gtgcctaccg cttgagtccg
     9661 acgctggaat ccttcctgcg ttatgacctg atgattgtcg acgtggagga tcgctgggga
     9721 catgaatatg aacgtcaggg caggggaccg gcacacaatc gctatgcgaa ggatctgacc
     9781 ttcggcatca actgggatct gagtccggat ctcaccgtgc gtgccgaatg gcatcatgtc
     9841 gaaggcacgg gctggctgag tccggccgag gcaaatctgc gcgagacgga ccggcgctgg
     9901 aatctcggca tgctggaact gtcgttccgg ttctgagtac gacctcagcg tccggactcc
     9961 gccatttccc tgagcagcgc ctgctggcgg gccagggaat ggccgccgat cacgacgaaa
    10021 ccggccagca ccgcgtcatt cgattcgaag cgcaggattc gtattccgtc ctcggtgagt
    10081 tcccgccaga cgccggtgag tgtcgggggc ggcgggcagg tcacgatcgg gcaggcgggc
    10141 gtcttgatcg tgaccggcat cggcggcaag gacatttcgg tcggcttgcc ggtcagcgtc
    10201 gaagccagtg cgcgggcggc ctgcacggcc ggcatgacgt atggccgcac catgccgtcg
    10261 atctgcgcgc agtcgccgag tgcatgaatg tctgcggctg aagtgcgaag ctgacgatcc
    10321 acgacgatgc cacgttcaca gatcagtccc gcaccggctg ccagagcggt ctgtggcctg
    10381 aggccgactg cggacagcac gagatcggca tcgagcgtgt cgccgtcttc ggtgctcagg
    10441 cgatatcgac cggcccgcct gtcgatggcc cggacccgat gtccgcaacg ccactgcacg
    10501 ccggtcgcgg ccagcgcctc ggccagctgt gtactcgcgg gtacgggcag caggccggcg
    10561 agcgggcgcg gtgccggatc gatcaccgtg acccggtatc cgcatgcggt caggtcattg
    10621 gcaaactcgc agccgatcag gccggcaccg agcagggcta catgtccgga tgcaggcagt
    10681 cgttcccgga aacggcggta atcgtcgagg tgattgatcg acaggacctc gtcgctggca
    10741 tcgccccaga aatgcagccg caccggttcg gcgccgatcg cgagcaccag ggagcggtag
    10801 tgatacagcc cgctgctgct cagcagcgtg cgcgagtccg gatcgatgcg ctctacgcag
    10861 gtgtgcggca cgatggtgat gccgagttgc ctcgccatgt cggcgcgagt cgaggcgatc
    10921 aacgtctccg gggtctgccc acgggccagc gcgttggaca gcatcggctt cgcatagaag
    10981 tcggcacgat cggcagcgat cagcgtgatg tcgatctgcg catcgcggcg gcgcagctct
    11041 cgggcgagtc catacccggc gatgccggct ccgacgatga cgatgggatg catgtgccgg
    11101 gctcccgacg ctgtgctgat cattcgttga tatgccgggg cgatccgcgg atcgccccgg
    11161 caaccccggc agcttaggtg agcgcatgag gctgtacgcg ggaggatgtc tgcggcaccg
    11221 gcgtgcagca gggcaggcag ggcatccggg cgatggtgaa ccgccgcaaa cgggtgatca
    11281 ggtaatttgt caccggccct gatcgagccg ggcccgggcg gtaggcgtgg catgctccgg
    11341 cgtccagacc ggtgatcggc acagttcgac ctgcatcgcc gtgcccgccg gcagcagcgg
    11401 ggccagcgca caatggacgg cctcgatgat cgtggtgcct gccggacagg ccggggtcgt
    11461 catcgtcatg cagatgcgca ggcggtcttc ttccctgaaa atgccatgga ccagaccgag
    11521 ttcgatgatg ctgatgccgg tttcggggtc gatgacgcgt tcgagtcgtg ccagcacctc
    11581 ggcatcggtg gggctgcgga catgcatggc cggcctcaac ggatgaatgc tcgggaaaca
    11641 gcctgcatgc tagcgtcccc ggcccggcac ccgttgatgc acagggagta acccggtcgg
    11701 gcggccagcc ggggtggcca cgcatgggca tggggctgga gcttgccgga atcattcgat
    11761 caggtggtgg catcgctgac cgaccggtca gctgaattcc tcttcctgtc catgatctac
    11821 cagaatgccg atgcggcaga ccacccccgg cgccggaccg gattccaggt attccaaatc
    11881 attcagaaaa tgaccgttct caaggagaac tgaacgatga tccgccgcag ccatccgccg
    11941 gccttccggc ttgccaccct cctcggcgcc atgctggccg gtgtgctctg gaccggcccg
    12001 gcgatcgccg atcagcgcat ccgctgcgaa tctgtcgatg gacgccagaa ttactgctcg
    12061 gcggataccc gtggaggtgt gcggctggct cgccagctca gcaggagtga ttgctacgaa
    12121 ggacgcacct ggggttatga ccggcgcggg gtctgggtca gcgaaggctg ccgggccgag
    12181 ttcatcgtgt tcgaaccgag aggtcggggc tatccggacg atgactgggg cgggcgcggt
    12241 gatcgtggtc gacacggggg ctgggatgat gagcgtcggg gttatggccg tggcccgcgc
    12301 gaggatgtgc gcgtgcgctg cgagtccgac cggcaccgct atacgagctg tcgcatcgat
    12361 atcgggaacg ggcgcgtcga actggtgcgc cagttctcgg acgaggattg tcggtacgac
    12421 cgcagctggg gttacgaccg ccgccagatc tgggtcgatg gcggttgccg tgccgaattc
    12481 gtgatcatcc gctgatgccg gccgaagcac agaatccgcc cgcggcgcgt tcgtgcgcgc
    12541 cggtcgtggt gctcgacttc gaaacgaccg gcatgagccc gggccacggc gaccgcatca
    12601 ccgaagtcgg tgccgtgcgc atcgaggacg gctgcatcac ggcacgcttc cagagtctcg
    12661 tgcgcaccgg cgtgccggtg ccgccgttca tccggcagct gaccggcatc agcgatgcga
    12721 tgctggcagg ggcaccgccg gcagaagagg tcctggcgca actcgtcgag ttcatcggcc
    12781 gggacgcgct ggtcgcacac aacgcgagct tcgaccggcg tttcctcgat gccgaactgg
    12841 cgctgatcgg ggcggcgcgt atccagccgg tgttctgcac ggtgctgctg tcccgccggc
    12901 tgctgccgca ggcgccgagc cacagcctcg gagcgctggt gagctggctc ggactgccga
    12961 gtgacggcgt tcatcaccgt gcactcgccg atgccgagat gaccgcgcac ctgtggctgc
    13021 atctgcatca ggtgctcgat cagcgctatg gcttcagcca ggtgtcgctt gcctggctgc
    13081 agaaactcat cgaccggtca ccgcgcagcg ccgacacctg gctgcgcagt cagacagtcg
    13141 aaatcgtgcg tgcatgagct gaagggcagg gccgatcccc ggttcaggcc ggccgccggc
    13201 gccggtcacc gcgatccgca cggcgcggac cgtcggcacg cgccgacggc tccgggcggg
    13261 tcaccgctgg ccggccgccg ccgtcacgtc cttgcggcgc ccgggcccgg gtggcagg
//
LOCUS       DAOQIE010000064        13267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2278_length_13267_cov_7.286633, whole genome shotgun sequence.
ACCESSION   DAOQIE010000064 DAOQIE010000000
VERSION     DAOQIE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_13267_cov_7.286633"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..534)
                     /locus_tag="PK144_05870"
     CDS             complement(151..534)
                     /locus_tag="PK144_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNF65289.1"
                     /translation="MDCLDRIAEERILQAQREGAFENLPGNGKPLVLDDDSMIPPELR
                     AAYRILKNAGYAPPEVLARRDIADLEAAITDPATDDPTRRQAIARLHLLRARLGATGR
                     DLRIEQAYWERLVERMTHGGKSSGG"
     gene            complement(579..1424)
                     /gene="modD"
                     /locus_tag="PK144_05875"
     CDS             complement(579..1424)
                     /gene="modD"
                     /locus_tag="PK144_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HNF65290.1"
                     /translation="MSLADLPDTELARWLDEDVPYLDLTTHGLGIGRTPGRAHFAARQ
                     PMTVCAIEEAARLFELCGCTVERRVDSGTAVAAKTPLLSAHGPAAGLHRAWKTAQTLV
                     EIASGIASGASALVAAVRGVGCEPRIACTRKNVPGTRRLAVKAIVAGGATPHRLGLSD
                     SVLVFAEHRCFVPAAELAGRLAALKREYPERRIVAEVSDIDEALALAALGLDGLQLEK
                     FEPAAISELIARLGRPRPQLLAAGGINPANAAAYAATGADVLVTSAPYHAPPRDVAVT
                     LEAAG"
     gene            1668..2462
                     /locus_tag="PK144_05880"
     CDS             1668..2462
                     /locus_tag="PK144_05880"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNF65291.1"
                     /translation="MSSHNPNTPVAVVTGSTSGIGLGIARKLAEHGHALVLNGLSTPM
                     EADALCTQFMADFGVEAVFSGADLRKPEGVAELIDTATMRFGRLDVLVNNAGIQHTAP
                     VQEFPVEKWDAILALNLSAAFHAIRLALPGMLERGFGRIVNIASVHGLVGSAHKAAYV
                     AAKHGIVGLSKVVALETAGLGVTCNAVCPGWVLTPLVEKQIADRAAVEGIGIEQAGRE
                     LLEEKQPSGQFVTPEQLGELVVFLASGAAAQLTGQALAVDGGWTAR"
     gene            2578..3609
                     /locus_tag="PK144_05885"
     CDS             2578..3609
                     /locus_tag="PK144_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF65292.1"
                     /translation="MTANPQDGIHRINIALQGGGAHGAFTWGVLDRLLEDGRLLIDGI
                     SGTSAGAMNAAVLAYGLARGGPPAARAALDEFWRRTSAASAYSPMQPSWFDRWIGNGG
                     MEWSPAYFVFDTLTRFFSPYQLNPMNTNPLRDVLNAVVDFDVLRRAQDVKLFLCATNV
                     RRGRIRVFETHEIGPEAVLASACLPFLFQAVEIDGEHYWDGGYMGNPPIYPLIYGTDS
                     RDVLIIRINPIEIAEVPRTAREIMDRVNTLSFNSSLMREMRAIGFVTRLIDEGALDPA
                     QYKRLRIHSIDAEERMAQLGVSSKLNADWRWLCELRELGRQKAQQWLARHFGDVGVRS
                     STDVATMFL"
     gene            3630..5753
                     /locus_tag="PK144_05890"
     CDS             3630..5753
                     /locus_tag="PK144_05890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF65293.1"
                     /translation="MHVHAETFARATRALREHRSARKLARVLHALQKPAAALWPFAEL
                     GVRLALAQVFLVSGLLKLADWPTALRLASLEYPVDWLAPATAAVLGVTAELLGGILLA
                     AGLATRAAAVALIALTLVIDLEYRALAEHRDWLVLLGGWLLLGAGPLSLDRLLARGLS
                     SSALPGAARLQAGLAAVSAAGEAPWLLVVRGWLAWRVLDTQWPGFSGAPLAGAWPCLL
                     GAALVLAGLGTRLFAPALLLIFAAASLPDFGWLAGLALLLALRGAGAWSLDGLIAAAI
                     ERRAPRLSALPDEVVDGLPQVVVVGAGFGGLAVARGLAEAPCRVTVVDKRNHHLFQPL
                     LYQVATATLDPSDIATPIRELLRTQDNARVRLGEAAGVDRDARRLRLADGSTLPYDFL
                     VLATGARHSYFGRDDWEPYAPGLKQIDDATAIRRRILLAFERAETSTDPVERARLLTF
                     VIVGGGPTGVELAGSIAELAHHGLSGEFRHIDPAAARVILIQADARVLPAFPEVLSAR
                     AQRDLERLGVEVLTSARVEAVDADGVLVSGTRIGARAVFWAAGVMASPAAQWLGLEAD
                     RAGRVPVAADLSVEGWPGVYAIGDTAASLGWNGRPVPGLAPAAKQGGAYVATAIRARL
                     EGRDAPAPFRYVHQGSLATIGRQSAIADFGRVRLAGGLAWWLWGAVHVAFLAQLRSRV
                     SVAISWFWAYLTFRRGTRLVTGGDG"
     gene            5905..6708
                     /locus_tag="PK144_05895"
     CDS             5905..6708
                     /locus_tag="PK144_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF65294.1"
                     /translation="MDTQLLRTFLEVARTRHFGRAAAALYLTQSAVSARIRQLEDAVG
                     APLLERNRREVRLTAAGERFAGHAAELLASWSQICREARLAAGSGDGITLGAPAALWE
                     GGLAEALALPFAAQPALALDAVTAPPAVLVQRVLDGTLDLAFVSQPPAGEGLATRLYG
                     VQEWLLVAGTRGADGPIDIEAGPPLPDAGEAASSPRRVASVPLARALLLHGGGPAWLP
                     AALAVPLLDAGRLTRLDEVGICRLSIHALHARRSPRLVTVLALLEQPQA"
     gene            complement(6818..7216)
                     /locus_tag="PK144_05900"
     CDS             complement(6818..7216)
                     /locus_tag="PK144_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNF65295.1"
                     /translation="MSRYLWQPERHALGIEAMDRTHAECLALARALEECPQDQLAPRF
                     AELIGHTEAHFAAEEARMQASACPSTPEHQAEHRRVLGDLRRFASSLARGRPAMLRAY
                     LAEGFAEWLEQHVATMDAALAAHLKACGES"
     gene            complement(7397..>7633)
                     /locus_tag="PK144_05905"
     CDS             complement(7397..>7633)
                     /locus_tag="PK144_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65296.1"
                     /translation="LLRRDQLLSHSHFGADEPEPLAQRAVWPDGQLVAHCWSWLSLVA
                     VHEESACTPAEASMASSSADRRIGVDIAVFSFSG"
     assembly_gap    7634..7733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7911..11786)
                     /gene="purL"
                     /locus_tag="PK144_05910"
                     /gene_synonym="purI"
     CDS             complement(7911..11786)
                     /gene="purL"
                     /locus_tag="PK144_05910"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNF65297.1"
                     /translation="MLRFRGSPALSAFRRDKLLAAVQARVPAVRGVYAEYVHFVDGRL
                     DEAGTAVMTQLLEYGPALEARTGTTPPLLLVVPRPGTISPWSSKATDIAHACGLDEVR
                     RVERGIAWWFDGAGAADRPLLAPLVHDRMVEAVLADDDDPAVLFTQAEPRPLASVDVL
                     GGGREALVAANRSLGLALSADEIDYLVTNFTQLGRNPNDVELMMFAQANSEHCRHKIF
                     NADWIIDGETQPVSLFGMIKNTYRVAPAGVRSAYHDNAAVIDGHPAQRFFPDAQTAEY
                     RTSAEPLHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGSKPKAGLCGFSVS
                     NLHLPDAPQPWEGTPAKPGRIASALEIMLDGPIGAAAFNNEFGRPNLCGYFRSFELDG
                     PDGERRGYHKPIMIAGGYGSIREALIDKAGVPAGAAIVVLGGPAMLIGLGGGAASSMA
                     TGASSEDLDFASVQRGNPEIQRRAQEVIDRCAALGDANPILSIHDVGAGGLSNAVPEI
                     VHGAGRGGRFELRKVPSDDRGLSPLEIWCNEAQERYVLALDAARLPVFEAIAARERCP
                     YAVLGEAIEAGQLTVSDALHGNAPVDMPMQVLLGKPPKMLRDVQRVAVAGRPLALAGV
                     TVASAAGRVLRLPAVADKSFLITIGDRTVTGLVARDQMVGPWQVPVADCAVTLTDYHG
                     HTGEAMAMGERTPLAVLDAPASGRMAVAEAILNLAAARIGKLGDVKLSANWMAAAGHP
                     GEDARLFDTVRAVGLEFCPALGIAIPVGKDSLSMQTRWQEADGTAKRVTSPLSLIVSA
                     FAPVLDARATLTPQLRTDAGDTDLILVDLGRGKNRLGGSALAQCHGQTGDTVPDCDSP
                     EDLAALFAAVQQLNAAGRLLAYHDRSDGGLLVTLAEMCFAGGCGATVLLDDLDADPLA
                     AAFSEEAGVVLQVRAADRDAVLRALAAAGLGDCTHVIGAPDTGDRLRLLHGGEAVLDQ
                     RRSELRAAWSETSWRMQRLRDHPECADQAYALACDAADPGLSVQLTFDPTSDVAAPFI
                     ATGARPRVAVLREQGVNGQIEMAAAFDRAGFTAVDVHMSDILGGRVELAGFRALVACG
                     GFSYGDVLGAGEGWAKSILFNARAREQFEAFFARPDTLALGVCNGCQMMSNLHGIIPG
                     ADLWPRFVRNRVEQFEARLARVAVLESPSAFLAGMAGSLLPIAVAHGEGRAEFREGSG
                     PKKVLSAGLACLQFVDHHGEPTETYPLNPNGAPAGITGLTTHDGRVTILMPHPERVFR
                     TVQHSWHPAGWGEDGPWLRMFRNARKWLG"
     gene            11969..12667
                     /locus_tag="PK144_05915"
     CDS             11969..12667
                     /locus_tag="PK144_05915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF65298.1"
                     /translation="MTVAIRALSFDLDDTLWNIWPCIERAEAKLHDWLLAGYPRIAAQ
                     FDALALRRLCSDEATRDPAIAHDRSLIRRRGLTRAAELAGYAEFALDEAFEVFFAARN
                     QVEFFADALPVLERLAARYPVVALSNGNADLDRIGIAHLFAFHLSAAIVGTQKPHPAM
                     FEQAVARLGLPPAAILHVGDDPEHDVEGARAAGLRAVWVRRDGRDWPHGLAPPEFVIH
                     DLHELEALLATWPG"
     gene            12655..13155
                     /locus_tag="PK144_05920"
     CDS             12655..13155
                     /locus_tag="PK144_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNF65299.1"
                     /translation="MAGLSGPLGQLLATLPQTGRVDWIGLRPAPRAPMQEVDAVLAEA
                     GRGLAGDRWAGRPDGARQLTLLQAEHLPVIAGCAGLAAVEPARLRRNLVISGLNLLAL
                     KGRRFRLGEALCEYSGLCQPCSRMEAALGPGGWNAMRGHGGITARVLEGGWVRIGDAL
                     AVEHLP"
BASE COUNT         1756 a         4677 c         4867 g         1867 t
ORIGIN      
        1 ggcgatccga cgatgttttt cacggctgct cccgaaggcg acggcaaaca caaaggattt
       61 atagcgcaat aaatccatag cgcaaaaatt tctttgtgtt cggcggcggt tcactgccac
      121 ggaccacagc catcggcgaa gccgggggca ctacccgccc gatgacttcc ccccgtgcgt
      181 catcctctcc accagccgct cccagtacgc ctgctcgatg cgcagatccc gcccggtcgc
      241 accgagccgg gcgcgcagca ggtgcaggcg ggcgatggcc tggcggcggg tcgggtcgtc
      301 ggtggcgggg tcggtgattg cggcttcgag gtcggcgatg tcgcggcggg cgagcacttc
      361 gggcggggcg tagccggcgt tcttgaggat gcggtaggcg gcgcgcagtt cgggcgggat
      421 catcgagtcg tcgtcgagca ccagcggctt gccgttgccg ggcaggttct cgaaggcgcc
      481 ctcgcgctgg gcctgcagga tgcgttcttc ggcgatgcga tcgaggcagt ccatcgggcg
      541 tctcctgcga tcgggtgggc ggagcggtcg ccggcggatc agccggcggc ttcgagggtc
      601 acggccacgt ccctcggcgg ggcgtggtag ggcgcggagg tgaccagcac gtcggcgccg
      661 gtggcggcgt aggcggcggc gttcgccggg ttgatgccgc cggcggccag cagttgcggg
      721 cgcgggcggc cgaggcgggc gatcagttcc gagatggcgg cgggctcgaa tttttcgagc
      781 tgcaggccgt cgaggccgag cgcggcgagc gccagtgcct cgtcgatgtc gctgacctcg
      841 gcgacgatgc gccgctccgg gtattcgcgc ttcagcgccg cgagccgccc ggccagttcc
      901 gcggccggca cgaagcagcg gtgctcggcg aacaccagca ccgagtccga cagcccgagc
      961 cggtgcggcg tggcgccgcc ggcgacgatg gccttgaccg cgagccggcg cgtgccgggc
     1021 acgttcttgc gcgtgcaggc gatgcgcggc tcgcagccga cgccgcgcac ggcggcgacc
     1081 agcgccgagg caccgctggc gatgcccgag gcgatctcga ccagcgtctg tgccgtcttc
     1141 caggcccggt gcaggccggc agccgggccg tgtgcgctga gcagcggcgt tttcgccgcg
     1201 accgccgtgc cgctgtcgac ccggcgctcg accgtgcagc cgcacagctc gaacagccgt
     1261 gcggcttcct cgatcgcgca gaccgtcatc ggctggcggg cggcgaagtg cgcgcgcccg
     1321 ggcgtgcggc cgatgccgag gccgtgcgtg gtcaggtcga gataaggcac gtcctcgtcg
     1381 agccagcggg cgagttcggt gtcgggcagg tcggcaagac tcatgttcgg gacccggaag
     1441 gtgcagggaa ggccggcagc gtacaccgcg gccggccgcg tccgcgcgtg gccggtctgc
     1501 gggatgtcgc ggtcgcgctc gcgggccgaa acggcgggga atcggcaagt cactgttctg
     1561 aaagcctgat gtgacctggc atgcggctcg ctcctaggga atcgaggtgt accgatacac
     1621 ccgtgccccg ataccacccg cccccaggag tcccgagcat gtccccgatg tcttcgcaca
     1681 accccaacac ccccgtcgcc gtcgtcaccg gttccaccag cggcatcggc ctcggcatcg
     1741 cgcgcaagct cgccgagcac ggccacgcgc tggtgctgaa cggcctgagc acgccgatgg
     1801 aagccgatgc gctctgcacg cagttcatgg ccgatttcgg cgtcgaggcc gtgttcagcg
     1861 gcgccgacct gcgcaagccc gaaggcgtgg ccgagctgat cgacaccgcg acgatgcgct
     1921 tcggccgcct cgacgtgctg gtcaacaacg ccggcatcca gcacacggcg ccggtgcagg
     1981 agttcccggt cgagaagtgg gacgcgatcc tggcgctgaa cctgagcgcg gccttccacg
     2041 cgatccgcct ggcgctgccc ggcatgctcg aacgcggctt cgggcgcatc gtcaacatcg
     2101 cctcggtgca cggtctggtc ggctcggcgc acaaggccgc ctacgtcgcg gccaagcacg
     2161 gcatcgtcgg cctcagcaag gtcgtggcgc tcgaaaccgc cgggctcggc gtgacctgca
     2221 acgccgtctg ccccggctgg gtgctgacgc cgctggtcga gaagcagatc gccgatcgcg
     2281 ccgcggtcga gggcatcggc atcgagcagg cggggcgcga gctgctggag gagaagcagc
     2341 cttcggggca gttcgtgacg ccggagcagc tcggcgagct ggtggtgttc ctggcctcgg
     2401 gcgccgcggc gcagctgacc gggcaggcgc tggccgtcga cggcggctgg acggcgcgct
     2461 gatcgcattc ccgatacccg actgcgggtt ctgtgccctc tctcccgcga ggggagagag
     2521 ggggctgaaa gcgcaggcgt ccggcacgtt ccggcagacg cgaggagaaa acccgacatg
     2581 accgccaacc cgcaagacgg cattcaccgc atcaacatcg cgctgcaggg tggcggcgca
     2641 cacggcgcct tcacctgggg cgtgctcgac cggctgctgg aggacgggcg gctgctgatc
     2701 gacggcatca gcggcaccag cgccggcgcg atgaacgccg cggtgctcgc ctacggcctc
     2761 gcgcgcggcg ggccgccggc agcgcgcgcc gcgctcgacg agttctggcg ccggacctcg
     2821 gcggcgtcgg cgtactcgcc gatgcagccg tcgtggttcg accgctggat cggcaacggc
     2881 ggcatggagt ggtcgccggc gtatttcgtg ttcgacacgc tgacgcgctt cttctcgccg
     2941 taccagctga acccgatgaa caccaatccg ctgcgcgacg tgctcaatgc cgtcgtcgat
     3001 ttcgacgtgc tgcgtcgcgc gcaggacgtg aagctgttcc tgtgcgcgac caacgtccgg
     3061 cgcgggcgca tccgcgtgtt cgagacgcac gagatcggcc ccgaggcggt gctggcctcg
     3121 gcctgtctgc ccttcctgtt ccaggcggtc gagatcgacg gcgagcacta ctgggacggc
     3181 ggctacatgg gcaatccgcc gatctacccg ctgatctacg gcaccgacag ccgcgacgtg
     3241 ctgatcatcc gcatcaaccc gatcgagatc gccgaggtgc cgaggacggc gcgcgagatc
     3301 atggatcgcg tcaacacgct gtccttcaac agctcgctga tgcgcgagat gcgcgcgatc
     3361 ggcttcgtca ccaggctgat cgacgagggc gcgctcgacc cggcgcagta caagcgcctg
     3421 cgcatccact cgatcgacgc cgaggagcgc atggcgcagc tcggcgtgtc gagcaagctc
     3481 aacgccgact ggcgctggct ctgcgagctg cgcgaactcg gccggcagaa ggcgcagcag
     3541 tggctggccc ggcacttcgg cgatgtcggc gtgcgctcgt cgaccgacgt cgcgacgatg
     3601 ttcctgtgaa cggcgaagga ggcacgacca tgcacgtcca cgctgaaacc ttcgcccgcg
     3661 ccacgcgcgc gctgcgcgaa caccgctcgg cacgcaagct cgcgcgtgtc ctgcatgcgc
     3721 tgcagaaacc cgccgcggca ctctggccgt tcgccgaact cggcgtgcgt ctggcgctcg
     3781 cgcaggtctt tctcgtctcg ggcctgctga agctcgccga ctggccgacg gcgctcaggc
     3841 tcgcaagcct tgaatatccg gtcgactggc tggcaccggc gacggccgcc gtgctcggcg
     3901 tcacggccga actgctcggc gggatattgc tggcagcggg cctggcgacg cgcgctgcgg
     3961 ccgtggcgct gatcgcgctg acgctggtca tcgatctcga ataccgcgca ctcgccgagc
     4021 accgcgactg gctcgtgctg ctcggcggct ggctgctgct cggcgccggg ccgctgtcgc
     4081 tcgaccggct gctggcgcgc gggctttcga gcagcgcgct gccgggggcg gcgcgcctgc
     4141 aggcagggct ggcggccgtg agcgcggccg gcgaggcgcc gtggctgctc gtcgtgcgcg
     4201 gctggctggc gtggcgggtg ctcgatacgc agtggccggg cttcagcggc gcgccgctcg
     4261 ccggtgcctg gccgtgcctg ctcggcgcgg cgctggtgct cgccgggctc ggcacgcggc
     4321 tgttcgcacc ggcgctgctg ctgatcttcg cagccgcgtc gctgccggac ttcggctggc
     4381 tcgccggcct cgcgctgctg ctcgcgctgc gcggcgccgg tgcgtggtcg ctcgacggcc
     4441 tgatcgcggc ggcgatcgaa cggcgcgcac cgcgcctgtc ggcgctgccg gacgaggtcg
     4501 tcgacggcct gccgcaggtc gtcgtcgtcg gcgccggctt cggcggactg gcggtggcgc
     4561 gggggctggc cgaggcgccg tgccgcgtga ccgtcgtcga caagcgcaac caccacctgt
     4621 tccagccgct gctctatcag gtcgcgaccg cgacgctcga tccgtccgac atcgccacgc
     4681 cgatccgcga actgctgcgc acgcaggaca acgcccgcgt gcggctcggc gaggccgccg
     4741 gcgtcgatcg cgacgcacgc cggctccggc tcgccgatgg ttcgacgctg ccgtatgact
     4801 tcctcgtgct cgccaccggt gcgcgccaca gctacttcgg ccgcgacgac tgggaaccct
     4861 acgcgccggg cctgaagcag atcgacgatg cgaccgcgat ccgccggcgc atcctgctcg
     4921 ccttcgagcg cgccgagacc agcaccgatc cggtcgagcg cgcccggctg ctcaccttcg
     4981 tgatcgtcgg cggcggtccg accggcgtcg agctggccgg ctcgatcgcc gagcttgcgc
     5041 atcacggcct gagcggcgag ttccggcaca tcgatccggc cgcggcgcgc gtgatcctga
     5101 tccaggccga tgcgcgcgtg ctgccggcct tcccggaggt gctgtcggcg cgggcgcagc
     5161 gcgacctcga acgcctcggc gtcgaggtgc tgacctcggc gcgcgtcgag gcggtcgatg
     5221 ccgacggcgt gctggtgtcc ggcacgcgca tcggcgcgcg cgcggtgttc tgggccgccg
     5281 gcgtcatggc ctcgccggcg gcgcagtggc tcgggctcga agccgaccgc gccgggcgcg
     5341 tgccggtcgc cgccgatctg tcggtcgagg gctggccggg cgtctacgcg atcggcgaca
     5401 ccgccgcgag cctcggctgg aacggccggc cggtgccggg gctggcaccg gctgcgaagc
     5461 agggcggggc gtatgtggcc acggcgatcc gcgcccggct cgaaggccgc gacgcaccgg
     5521 cgccgtttcg ctacgtgcat cagggcagcc tcgcgaccat cggccggcag tcggcgatcg
     5581 ccgacttcgg ccgcgtgcgc ctcgccggcg ggctggcgtg gtggctgtgg ggcgcggtgc
     5641 acgtcgcctt cctcgcgcag ctgcgcagcc gcgtgtcggt cgcgatctcg tggttctggg
     5701 cgtacctgac cttccgccgc ggcacgcggc tggtgaccgg cggcgacggc tgacagaggc
     5761 gttgcatgga aagtccggaa cgccccgctg caggcagccg gcggggcggc cggcatgcag
     5821 gacggcatcg gtcgcgcgat gaaatctgcc gccggctgac gcatgctgcg gcagaccgat
     5881 tcgcgcacgg gctcccgccg atccatggat acgcaactgc tgcgcacctt tctggaagtc
     5941 gcccgcaccc gccacttcgg ccgggccgcg gcggcgctgt acctgacgca atcggcggtc
     6001 agcgcccgca tccgccagct cgaagatgcg gtcggcgcgc cgctgctgga gcgcaaccgc
     6061 cgcgaggtgc gtctgaccgc ggccggcgag cgcttcgccg gacatgcggc cgaactgctg
     6121 gcgagctggt cgcagatctg ccgcgaggcc cggcttgccg ccggcagtgg cgacggcatc
     6181 accctcggcg cgccggcggc gctgtgggaa ggagggcttg ccgaggcgct ggccctgccc
     6241 ttcgccgcgc agccggcgct ggcgcttgat gccgtgaccg cgccgccggc cgtgctcgtg
     6301 cagcgcgtgc tcgacggcac gctggatctg gccttcgtca gccagccgcc ggccggcgag
     6361 gggctggcga cgcggctcta cggcgtgcag gaatggctgc tggtggccgg cacccgcggg
     6421 gccgacggcc cgatcgacat cgaggccggc ccgccgctgc cggatgccgg cgaggctgcg
     6481 tcgtcgccgc ggcgcgtggc atcggtacca ctggcgcgcg cgctgctgct gcacggcggc
     6541 ggtccggcct ggctgccggc ggcgctggcg gtgccgctgc tcgacgccgg ccggctcacg
     6601 cgcctcgatg aggtgggtat ctgccggctg tcgatccacg ccctgcacgc gcgccgttcc
     6661 cctcggctgg tgacggtact ggcgctgctg gagcagccgc aggcctgagc gaagcggcac
     6721 ggggcggagc cggcgttgcg aggaacggca gaggagcccg gagtgtccgg cagccgctcg
     6781 tgccggggag ggcgcgcggt tcaggaatcg cccgctttca gctttccccg caggccttca
     6841 gatgcgccgc cagcgccgca tccatggtgg cgacgtgctg ctccagccat tcggcgaagc
     6901 cctcggcgag gtaggcgcgc agcattgccg ggcggccgcg cgccagcgac gaggcaaagc
     6961 ggcgcaggtc gccgagcacg cgccggtgct cggcctgatg ctcgggcgtg ctcgggcagg
     7021 cgctggcctg catcctcgct tcctcggcgg cgaagtgcgc ctcggtgtgg ccgatcagtt
     7081 cggcgaagcg cggggcgagc tgatcctgcg ggcactcctc cagcgcgcgc gccagcgcca
     7141 gacactcggc gtgcgtgcgg tccatggctt cgatgccgag ggcatgtcgt tcgggctgcc
     7201 agaggtagcg gctcatgatc ggggctccgg agtcggggga agaggacgtg ccgagactcg
     7261 cacgggcccg ggacggccgg catgcgcagg atcagccatc cgctgcaatc ccggcagcgg
     7321 agggaacgcc ggggcctgtc tacgctccgc agcaggcagg tgccgcatgg cgcggccccg
     7381 atcgccccga agaagatcac cccgagaagg agaacaccgc gatgtcgacc ccgatgcgtc
     7441 tgtccgcgct gctgctggcc atgctggcct cagcgggcgt gcatgccgat tcctcgtgca
     7501 cggccaccaa cgacaaccag gaccagcagt gcgccaccag ctgtccgtcc ggccagacgg
     7561 cgcgctgcgc caacggctcg ggttcgtcgg cgccgaagtg cgagtgcgag agcaactgat
     7621 cccgccgcag cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggttc
     7741 gtcggcgccg aagtgcgagt gcgagagcaa ctgatcccgc cgcagcagcc ggaatccgtt
     7801 ttctccttct ttccctcgtc ccttcagcct tctcccgcga cgaaagaggg ctgaaggaga
     7861 cgtgccggca gagtccggcg gattccggcg gattcaggat gcccagccgc tcagcccagc
     7921 cacttgcgcg cgttcctgaa catccgcagc cacggtccgt cctcgcccca gccggccggg
     7981 tgccaggaat gctgcacggt gcggaacacg cgctccgggt gcggcatcag gatcgtgacg
     8041 cggccgtcgt gcgtggtcag gccggtgatg ccggccgggg cgccgttcgg gttcagcggg
     8101 taggtttcgg tcggttcgcc gtggtgatcg acgaactgca ggcaggcaag cccggccgac
     8161 agcaccttct tcgggcccga gccttcgcgg aactcggcgc ggccctcgcc gtgcgcgacc
     8221 gcgatcggca gcagcgagcc ggccatgccg gcgaggaagg ccgacggcga ttcgagcacc
     8281 gcgacgcgcg cgagccgcgc ctcgaactgc tcgacgcggt tgcgcacgaa gcgcggccac
     8341 aggtcggcgc cggggatgat cccgtgcagg ttcgacatca tctggcagcc gttgcagacg
     8401 ccgagcgcga gcgtgtccgg gcgcgcgaag aaggcttcga actgctcgcg ggcgcgggcg
     8461 ttgaacagga tcgacttggc ccagccttcg ccggctccga gcacgtcgcc gtacgaaaac
     8521 ccgccgcagg cgaccagcgc gcggaagccg gcgagctcga cgcgcccgcc gaggatgtcg
     8581 ctcatgtgca cgtcgacggc cgtgaagccg gcgcggtcga aggccgcggc catctcgatc
     8641 tggccgttga cgccctgctc gcgcagcacc gcgacccgcg gccgggcgcc ggtggcgatg
     8701 aagggcgcgg cgacatcgct ggtcggatcg aaagtgagct gcaccgacag gcccgggtcg
     8761 gcggcatcgc aggcaagcgc gtaggcctgg tcggcacatt cggggtgatc gcgcagccgc
     8821 tgcatccgcc acgaggtttc cgaccacgcg gcgcgcagct cgctgcggcg ctggtcgagc
     8881 acggcttcgc cgccgtgcag aagccgcagc cggtcgccgg tgtccggggc gccgatcacg
     8941 tgcgtgcagt cgccgaggcc ggcagcggca agcgcgcgca gcaccgcgtc gcggtcagcg
     9001 gcgcgcacct gcagcacgac gccggcttcc tcgctgaagg ccgccgccag cgggtcggcg
     9061 tcgagatcat cgagcagcac ggtcgcgccg cagccgccgg caaagcacat ctcggcgagc
     9121 gtcaccagca ggccgccgtc ggagcggtcg tgatacgcga gcagccggcc ggcggcgttc
     9181 agctgctgca cggccgcaaa cagcgcggcg aggtcttcgg gcgaatcgca gtccggcacc
     9241 gtgtcgccgg tctggccgtg gcactgcgcg agcgccgagc cgccgaggcg gttcttgccg
     9301 cggccgaggt cgacgaggat caggtcggtg tcgccggcgt cggtgcgcag ctgcggcgtc
     9361 agcgtcgcgc gcgcatcgag caccggcgcg aaggccgaga cgatcagcga cagcggcgag
     9421 gtcacgcgct tcgccgtgcc gtcggcctcc tgccagcggg tctgcatcga cagcgaatcc
     9481 ttgccgaccg ggatggcgat gccgagcgcc gggcagaatt ccagcccgac cgcgcgcacg
     9541 gtgtcgaaca gccgcgcgtc ctcgcccggg tggccggccg cggccatcca gttggccgac
     9601 agcttcacat ccccgagctt gccgatgcgc gcggcggcga ggttcaggat cgcctcggcg
     9661 accgccatgc gtccggaggc cggcgcatcg agcacggcga gcggcgtgcg ctcgcccatc
     9721 gccatcgcct cgccggtgtg gccgtggtag tcggtcagcg tcaccgcgca gtcggcgacc
     9781 ggcacctgcc acgggccgac catctggtcg cgcgcgacca gaccggtgac ggtgcgatcg
     9841 ccgatcgtga tcaggaagga cttgtcggcg accgccggca ggcgcagcac gcgtccggcg
     9901 gccgaggcga ccgtgacgcc cgcgagcgcg agcggccggc ctgcgacggc gacgcgctgc
     9961 acgtcgcgca gcatcttcgg cggcttgccg agcagcacct gcatcggcat gtcgaccggc
    10021 gcgttgccgt gcagcgcatc gctgacggtc agctgtccgg cctcgatcgc ctcgccgagc
    10081 accgcgtacg ggcagcgttc gcgcgcggcg atggcctcga acaccggcag gcgcgcggcg
    10141 tcgagcgcga gcacgtagcg ctcctgcgcc tcgttgcacc agatctccag cggcgacagg
    10201 ccgcggtcgt cggagggcac cttgcgcagc tcgaagcgcc cgccgcggcc ggcgccgtgc
    10261 acgatctcgg gcacggcgtt ggaaagcccg ccggcgccga catcgtggat cgacaggatc
    10321 gggttggcgt cgccgagcgc ggcgcagcgg tcgatgacct cctgcgcgcg gcgctggatc
    10381 tcggggttgc cgcgctgcac cgaggcgaag tcgaggtcct cgctcgaagc gccggtcgcc
    10441 atcgacgagg ccgcgccgcc gccgaggccg atcagcatcg ccgggccgcc gagcacgacg
    10501 atggcagctc cggccggcac gccggccttg tcgatcagcg cctcgcggat cgagccgtag
    10561 ccgccggcga tcatgatcgg cttgtgatag ccgcgccgct cgccgtccgg gccgtcgagc
    10621 tcgaagctgc ggaagtagcc gcagaggttc ggacggccga actcattatt gaaggccgcc
    10681 gcgccgatcg ggccgtcgag catgatctcc agcgccgagg cgatgcgccc gggcttcgcc
    10741 ggcgtgcctt cccagggctg cggcgcatcg ggcaggtgca ggttcgacac cgagaagccg
    10801 cagaggccgg ccttcggctt cgagccgcgc ccggtcgcgc cctcgtcgcg gatctcgccg
    10861 cccgagccgg tcgccgcccc cgggaacggc gagatcgcgg tcgggtggtt gtgcgtctcg
    10921 accttcatca ggatgtgcag cggctcggcc gaggtgcggt actcggccgt ctgcgcatcg
    10981 gggaagaagc gctgcgccgg gtggccgtcg atgaccgcgg cgttgtcgtg gtaggccgag
    11041 cgcacgcccg ccggcgcgac gcgataggtg ttcttgatca tgccgaacag cgacaccggc
    11101 tgcgtctcgc cgtcgatgat ccagtcggcg ttgaagatct tgtgccggca gtgctcggag
    11161 ttggcctgcg cgaacatcat cagctcgaca tcgttcgggt tgcgcccgag ctgcgtgaag
    11221 ttcgtgacca ggtagtcgat ctcgtctgcg gacagcgcga ggccgagcga acggttggct
    11281 gcgaccagcg cctcgcgacc gccgccgagc acgtcgacgc tcgccagcgg ccgcggttcg
    11341 gcctgcgtga acagcacggc cggatcgtcg tcgtcggcga gcacggcctc gaccatgcga
    11401 tcgtgcacga gcggcgccag cagcggacga tcggccgcgc cggcgccgtc gaaccaccag
    11461 gcgatgccgc gctcgacgcg gcgcacttca tcgagcccgc aggcgtgcgc gatgtcggtg
    11521 gccttcgacg accacggcga gatcgtgccc ggccgcggca cgaccaagag cagcggcggc
    11581 gtcgtcccgg tgcgggcttc gagcgccgga ccgtattcga gcagctgcgt catcaccgcc
    11641 gtgccggcct catcgaggcg gccgtcgacg aaatgcacgt attcggcgta gacgccgcgc
    11701 accgcgggca cgcgcgcctg cacggccgcg agcagcttgt cgcggcggaa ggcggaaagg
    11761 gcgggggaac cacggaagcg cagcatgggc gatcggcctc tcggcaaacg gcggggggcg
    11821 gggcggaaaa ggcgcggaat tgtaccggat gcgccgcagc acggcggtcc gcccggcggg
    11881 cacgcgcgcg ctgccgtgcg cggattcatg cgaaagtcac gcgcccgggg tcccaacccg
    11941 tcgatcgcgt ccgaccgaga cctgcgccat gaccgtcgcc atccgcgccc tgagctttga
    12001 cctcgacgac acgctgtgga acatctggcc gtgcatcgag cgcgccgagg cgaagctgca
    12061 cgactggctg ctcgccggct atccgcgcat cgccgcgcag ttcgatgcgc tggcgctgcg
    12121 ccggctctgc agcgacgagg ccacccgcga cccggccatc gcccacgacc gcagcctgat
    12181 ccgccgccgc ggcctgacgc gcgccgccga actcgccggt tacgccgaat tcgcgctcga
    12241 cgaggcgttc gaggtgttct tcgcggcgcg caaccaggtc gagttcttcg ccgatgcgct
    12301 gccggtgctc gaacgcctgg ccgcgcgcta cccggtcgtc gcgctcagca acggcaacgc
    12361 cgatctcgat cgcatcggca tcgcacacct gttcgcgttc cacctgtcgg cggcgatcgt
    12421 cggcacgcag aagccgcatc cggcgatgtt cgagcaggcc gtcgcgcgcc tcggcctgcc
    12481 gccggcggcg atcctgcacg tcggcgacga cccggagcac gatgtcgaag gcgcgcgcgc
    12541 ggccggtctg cgcgcggtct gggtgcggcg cgacgggcgc gactggccgc acggactcgc
    12601 gccgccggag ttcgtgattc acgacctgca cgaactcgaa gccctgctcg cgacatggcc
    12661 gggctgagcg ggccgctcgg gcaactgctg gcgacgctgc cgcagaccgg gcgcgtcgac
    12721 tggatcggcc tgcgcccggc gccgcgtgcg ccgatgcagg aggtcgatgc ggtgctcgcc
    12781 gaggccggcc gcggcctggc cggtgaccgc tgggccggcc ggcccgacgg cgcacgccag
    12841 ctcacgctgc tgcaggccga gcacctgccg gtgatcgccg gctgtgccgg gctggccgcg
    12901 gtcgagccgg cacggctgcg gcgcaatctc gtgatttccg gcctgaacct gctggcgctg
    12961 aagggccggc gcttccgcct cggcgaggcg ctctgcgaat acagcggcct gtgccagccc
    13021 tgctcgcgca tggaagccgc cctcggaccg ggcggctgga acgccatgcg cggccacggc
    13081 ggcatcaccg cgcgcgtgct cgaaggcggc tgggttcgca tcggcgatgc gctcgcggtc
    13141 gaacacctgc cgtgatttgc tccggagaca gcgctgcttg ccgtgactca cgccgcgccc
    13201 ggccatgctc ggggctccgc ccagacctgg cccttgcgcc acggagtcct gcatgtccgc
    13261 gacccgc
//
LOCUS       DAOQIE010000065        13120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2311_length_13120_cov_7.790662, whole genome shotgun sequence.
ACCESSION   DAOQIE010000065 DAOQIE010000000
VERSION     DAOQIE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2311_length_13120_cov_7.790662"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PK144_05925"
     CDS             complement(<1..777)
                     /locus_tag="PK144_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65300.1"
                     /translation="MDRIRILIVDDAVVVRRMLSTLLAEDPELEVVGTAVNGADALRK
                     IPDLRPHLLILDVDMPEMNGLETLTVLGREHPRLPVIMFSNLTRRGAQTTLEALALGA
                     KDYVTKPESVGSAGEALEQVRAALVPKIKALCRPWEPAPAVLARRSHEPPAARQAPPS
                     APAPAPAAPVSPAAHASAPPLPSPVLAPMTRRGGIELVVIGCSTGGPNALATLVAALP
                     RGLAVPVVVVQHMPPVFTRMLAERLDALSPLSVREGSDGAR"
     gene            complement(1047..1487)
                     /locus_tag="PK144_05930"
     CDS             complement(1047..1487)
                     /locus_tag="PK144_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNF65301.1"
                     /translation="MSDDAQYCTFRLGSLFFGVEVTKVQEVIRYQEMTPIPLAQRTVS
                     GLINLRGQIVTAIDLRRVLEFEDRPADERPMNVVIRTDEGAVSLLVDRIGDVMHVDSD
                     SFERVPDTVTGVARDLILGAYKLPDTLLLVLDTDRIVGSDDGSH"
     gene            complement(1487..4261)
                     /locus_tag="PK144_05935"
     CDS             complement(1487..4261)
                     /locus_tag="PK144_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNF65302.1"
                     /translation="MDELDDIVREFLAESNENLDRLDNEFVMLESSPDDRDTLASIFR
                     TIHTIKGTCGFLGFSHLEKVTHAGENLLSKLRDGHIRLNQERTTALLQMVDAVRAMLA
                     AIESTGSDGDQNYPELTERLLTLQRKEEAVVAPVAKTPAPPPPPPVPTEVAEGMDEIV
                     VEFLTESQENLDRLDNEFVALEAAPDDRDTVASIFRTIHTIKGTCGFLGFSKLEKLTH
                     AGENLLSKLRDGRIRLNQERTTALLKMVDAVRVMLGSIGASGHDGNETYPELIELLTA
                     LQSDGAAPAAAAPAAKPVVPALGEILVQQGVVAPERVDAALSKQDAGDPRHVGEILVQ
                     EEAVKPAAVVEALKAQKDVAKEVKAPAVAETSIRVDVGLLDHLMNLVGELVLTRNQVL
                     QYSKSADDSRFAATSQRLNLITTELQEGVMKTRMQPINNIWNKVPRVVRDLALGVSKQ
                     IRVEMVGKETELDKSLIEAMKDPLTHLVRNCVDHGIEAPDVRVQKGKPAEGVLTLRAY
                     HEGGQVIVEIADDGAGIDPQKLKSKALQKGLITAEQAEAMSARELINLIFLPGFSTAE
                     KVTNISGRGVGMDVVKTNIEKISGNIELRSELGHGTLFKINLPLTLAIVPALIVTSGG
                     QRFAIPQGSLQELIRLDAAQGIEQIHGAHVYRLRGKLLPLVYLNRELGLEGATRNANI
                     VVLQSDKRRFGLVVDEINDTQEIVVKPLSKLLKGLTVFAGATVMGDGRVVLILDILGL
                     AQRARIVGETRDHGAAESIANGDVSGEAQNMLLVRSPGDGRLAIALSKVARLEEFPRN
                     SLEMAGSQEVVQYRKQILPLVRLEDVLPERRRFDRYEDMATLDESVVQVVVYTVNGRS
                     LGLVVDQIIDIIEENLDLTGDPSREGVLGTTVLQGRVTEVFDVDGFLERMFAALFPAR
                     PTARLSSTVY"
     gene            4551..5198
                     /locus_tag="PK144_05940"
     CDS             4551..5198
                     /locus_tag="PK144_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF65303.1"
                     /translation="MHIILVANPKGGSGKTTLAVNLAGYYAAHGRRTGLFDLDRQQSA
                     LRWLARRPANLTRISGFDAREDDTVIEDSAGLDVAIIDAPAALRGERFKDIVRIADHA
                     IIPVQPSVFDIDASAAFLERLIEQKSVRKARCLPGLIGNRVQARARSARQLETFIAGR
                     GLPALGALRDSQVYVQTAASGITLFELPGRRQAADRASWQPLLDWLAIPAADPPD"
     gene            5334..>6467
                     /locus_tag="PK144_05945"
     CDS             5334..>6467
                     /locus_tag="PK144_05945"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNF65304.1"
                     /translation="MPGFARCLVLLLCLLPWTSVPAAAETTKRPPEPPRPTLVGLVAG
                     GIGGSYARLADDLAAVLDAPDLRILPILGRGSVQNLRDLQALPFVDLALVQSDVLEHL
                     RADPAQEPLVRRLQVIAKLYNEEFHLLVRGHITRLSDLTGTKVNFDVPGSGTEITAAG
                     VFAALKLPVEPTHFDQLTALEKLRSGEISALAFVAGKPAPLFQRVRPEDDLHFLPITL
                     PPELLGRYLPTRFTKADYPNLIVGGDEIQTVAVGALLVTQATRKRAQLRKLELFTGRF
                     FDSFASFRQAGRHPKWREVSIAAQVPDWPRLKAAEDWLATNPDKVRSYAELAARAAAK
                     AEAARQEAARAETARIEAEKAEAARKAEVARIEAEKAEAARKAE"
     assembly_gap    6468..6567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6568..7072
                     /locus_tag="PK144_05950"
     CDS             <6568..7072
                     /locus_tag="PK144_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65305.1"
                     /translation="AEAEKAEAARAEAARRAEAEKAEAAKAEAAKQAEAAKAEADKAE
                     AARAEAAKQAEAAKAEADKAEAARKAETAKAEADKAEAARRAEAARAEAATKAEAARK
                     ADAAKAEAARKAEAEKAEATKAEADKTEAARKAEAARSAADRAEAERSGAESLPLTPV
                     NPAPVTP"
     gene            7133..8599
                     /locus_tag="PK144_05955"
     CDS             7133..8599
                     /locus_tag="PK144_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNF65306.1"
                     /translation="MSPLDRLPFDNRTARLPETLFARVAAQPHGRHHLVHASADALAL
                     LDLPPDITADPDFVEVFSGRRVPAGAEPIATVYAGHQFGIWVPQLGDGRALLLGELVN
                     RRGERWEVQLKGAGRTPYSRMGDGRAVLRSTIREYLGSEAMHHLGIPTTRAAAIIGSD
                     GPVLRESVESGAILVRLAPSHLRFGHFQFLYYSGRHELLRELLDFAIALHFPGLAALP
                     DAERHAALFANVIERTAQLVAGWQAVGFTHGVLNTDNMSLLGLTIDYGPFGFLDAYQP
                     GFVCNHSDDDGRYAFDRQPSIAWWNLSAFGQTLTPLVPVERLRALLDGFSEQYAQAYA
                     ARMRAKLGLREARDEDEALLEDLLGLMAGCGADYTNTLRSLCDFRPGTGPQALLDTVA
                     DPAGFETWLQRYGERLRAEASDDETRAANMRRVNPKIVLRNWLAQRAIEQAENGDYAG
                     IDRLLRLLQTPFDEHPEAPDCYGEPPDWARSLALSCSS"
     gene            complement(8615..9184)
                     /locus_tag="PK144_05960"
     CDS             complement(8615..9184)
                     /locus_tag="PK144_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65307.1"
                     /translation="MPVSPGFTACTTGLVAFWLSVLPCTPVLAEELALSLEPAQGPAL
                     VIGTVTLEADGDGERLTVAFDESRFEDHFLSMRPFRCLPAGEWLLCRLPYPYEHHRRI
                     RDSDLVDLEYALLFVIKPRSEYGIDLKDGLYWRLAREGRGFVGTPYAVDYTPLGVPPA
                     AGELRPLTHEQLWETEAQRLPFVRLRIGG"
     gene            complement(9267..10085)
                     /locus_tag="PK144_05965"
     CDS             complement(9267..10085)
                     /locus_tag="PK144_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF65308.1"
                     /translation="MTTLAGRTLFVTGASRGIGRAIALRAARDGANIAVIGKTETPDP
                     RLPGTIHDTAAEIEAAGGRALALAVDVRDESAVLAAVAATLRRFGRLDVLVNNASAIS
                     LGGTLDTPMKRFDLMMAVNARATFLCAQACIPQLLRSDNPHILTLAPPPVTAARWYGA
                     HLAYTLAKGGMSWVTLGLAEEFRAQGLAVNALWPRTVIATSAIRLLPGVDPARCRKPE
                     IVADAAHAILTRPARSCTGRFFLDEEALAEAGITDFDAYAVTPGAALLPDIFLD"
     gene            complement(10111..11442)
                     /locus_tag="PK144_05970"
     CDS             complement(10111..11442)
                     /locus_tag="PK144_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF65309.1"
                     /translation="MSASALPKPARTAGLSVLAALAATVAHGAGFQITESSVTGLGRA
                     FAGSGLVGDDLSAIAYNPAGLTLLDGTRIQGGATSISSDGEFRGRSTGTPSLRATGAP
                     IAGPVFSQDSSGGSSEDAGASAVVPNNYFVHALSPDLRVGFGLHSPFGLATDYGASWV
                     GRYHAQSSELQTVDLNPTLAWRLSPQLSLGFGLSYQLAKGRLSQAVFTGTPADGFAEF
                     KGDSDAFGWNLGAMYELAPGSRLGLSFRSKVTQDFDGDLRVRTPLGSRRFDAETSITL
                     PETVNLGYVQALDEQLSLSFGARWTNWSRFDELKLKSPGLAGGSKTFIEDWRDTWTLS
                     AGVDWRYSEAWTLRAGVAWDQTPVSSEEHRTARIPDADRWWLGFGASYRPTPKLTIDA
                     GFAHLFFEDADTRNVVTLDTGNPAIVIADSLSGSYSGSATLFGLQLQYRFD"
     gene            11918..>13120
                     /locus_tag="PK144_05975"
     CDS             11918..>13120
                     /locus_tag="PK144_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF65310.1"
                     /translation="MSFSDLSLDASLLRALTEAGYSEPTPIQAQAIPAALEGHDLIAS
                     AQTGTGKTAAFMLPALQRFAVASGDRRRAPRVLVLTPTRELAAQVMDAAHKYGRFLRF
                     TTVSLLGGMPYGPQLRALGGPLDLVVATPGRLIDHLDRDRIDLSKIEVLVLDEADRML
                     DLGFKEDVERIAEATPAGRQTLLFTATLDGAMARLAAELTRDAVRIAIEPTQTEQLNI
                     EQRLHVADDLAHKHRLLDHLLNDETMDRAIIFSATKRDADALAERLRDAGLPAAALHG
                     DMHQTLRSRTLARLRDGRVRLLVATDVAARGIDVQGITHVINFDLPKAAEDYVHRIGR
                     TGRAGRSGIAISLASYADVRQLERIERYTGKSIEEHVIEGLEPSRPLRRGRPGGGSGR
                     GRGGPRREG"
BASE COUNT         1965 a         4675 c         4482 g         1898 t
ORIGIN      
        1 gcgcgcgcca tcgctgccct cgcgcacgct cagcggcgac agcgcatcga gccgttcggc
       61 cagcatgcgc gtgaacaccg gcggcatgtg ctgcacgacc accaccggca ccgccagccc
      121 gcgcggcagc gccgcgacca gggtggcgag cgcgttcgga ccgccggtcg aacagccgat
      181 cacgaccagt tcgatgccgc cgcgacgggt catcggcgcc agcaccgggg acggcagcgg
      241 gggcgcggaa gcatgcgcgg cgggggaaac cggagcggcc ggtgcaggcg caggggcgga
      301 gggcggcgcc tgccgggctg caggcggttc gtggctgcgc cgggccagca ccgccggtgc
      361 cggctcccag ggccggcaca gcgccttgat cttcggcacc agcgcggcac gcacctgctc
      421 cagcgcctcg ccggcactgc cgacgctctc gggcttggtc acgtagtcct tggcgcccag
      481 ggccagcgct tcgagcgtgg tctgcgcgcc gcgccgcgtc aggttgctga acatgatcac
      541 cggcagccgc ggatgctcgc gcccgagcac ggtcagcgtc tccaggccgt tcatttccgg
      601 catgtcgaca tcgaggatca gcagatgcgg ccgcagatcg ggaatcttgc gcagcgcatc
      661 ggcgccgttg acggccgtgc cgacgacctc cagttcgggg tcttcggcga gcagcgtcga
      721 cagcatgcgc cgcacgacga ctgcgtcatc gacgatgagg atgcggatgc ggtccaaggc
      781 gggctccgga agcggcagtc acgtcaggcc gggcaggccg tgggtcgggc ccggcatggc
      841 acccggatat ggcttcggca cgtattcatc catcgttgcc ctctccgggg cccgacgcct
      901 gcggtgcggt tcggtttcgg tcgccaccgg ccccgtcgcc agccgcgcag acccggagcg
      961 acccgggccg cgcagcaaag cgcacccgag ccggtctgtc catgattctt gtgcctgcct
     1021 gcggccggaa accgggggcc ggaacttcag tggctgccgt cgtcgctgcc gacgatgcgg
     1081 tcggtgtcga gcaccagcag cagcgtgtcc ggcagcttgt aggcaccgag gatcagatcg
     1141 cgggccacgc cggtgacggt gtcgggcacg cgctcgaagc tgtcggaatc gacgtgcatc
     1201 acatcgccga tgcgatcgac cagcaggctg accgcgcctt cgtcggtgcg gatcacgacg
     1261 ttcatcggcc gttcgtcggc tggacggtcc tcgaattcga gcacccggcg caggtcgatc
     1321 gccgtgacga tctggccgcg caggttgatc agccccgaga ccgtgcgctg ggcgagcggg
     1381 atcggcgtca tctcctggta gcggatgact tcctggacct tcgtgacttc caccccgaag
     1441 aacaggctgc cgaggcggaa ggtgcagtac tgcgcgtcgt cgctcatcag tacaccgtgc
     1501 tgctcaggcg ggcagtcggc cgggccggga acagcgcggc gaacatgcgt tcaaggaaac
     1561 catcgacatc gaagacctcg gtgacacggc cctgcagcac cgtcgtgccg agcacgccct
     1621 cgcgcgaagg atcgccggtc aggtcgaggt tctcctcgat gatgtcgatg atctggtcaa
     1681 cgaccaggcc gaggctgcgg ccgttgaccg tgtagacgac gacctgcacg acgctctcgt
     1741 cgagcgtggc catgtcctcg tagcggtcga agcgccggcg ctcgggcagc acgtcctcga
     1801 ggcgcaccag cggcaggatc tgcttgcggt actgcacgac ctcctgcgag ccggccatct
     1861 ccagcgaatt gcgcgggaat tcctccaggc gcgcgacctt cgacagcgcg atcgcgagcc
     1921 gtccgtcgcc gggcgagcgc accagcagca tgttctgcgc ctcgccgctc acgtcgccgt
     1981 tggcgatgga ttcggccgca ccgtggtcgc gcgtctcgcc gacgatgcgc gcacgctgcg
     2041 cgaggccgag gatgtcgagg atcagcacga cgcggccatc gcccatgacc gtggctccgg
     2101 cgaacacagt caggcccttg agcagcttcg agagcggctt gacgacgatt tcctgcgtgt
     2161 cgttgatctc gtcgaccacc agcccgaagc ggcgcttgtc ggactgcagc acgacgatgt
     2221 tggcgttgcg cgtcgcgcct tcgagcccga gttcgcggtt caggtacacg agcggcagca
     2281 gcttgccgcg cagccggtag acgtgggcac cgtggatctg ctcgatgccc tgcgcggcgt
     2341 cgaggcggat cagctcctgc aggctgccct gcgggatcgc gaagcgctgg ccgccggagg
     2401 tcacgatcag cgccggcacg atcgccagcg tcagcggcag gttgatcttg aacagcgtgc
     2461 cgtgaccaag ctcgctgcgc agctcgatgt tgccgctgat cttctcgatg ttggtcttga
     2521 ccacgtccat gccgacgccg cggccggaga tgttcgtgac cttctcggcg gtcgagaagc
     2581 cgggcaggaa gatcaggttg atcagctcgc gcgccgacat cgcctcggcc tgctcggccg
     2641 tgatcaggcc cttctgcagc gccttggact tgagcttctg cggatcgatg ccggcaccgt
     2701 catcggcgat ctcgacgatg acctgcccgc cctcgtggta ggcgcgcagc gtcagcacgc
     2761 cctcggcggg cttgcccttc tgcacgcgca cgtccggcgc ctcgatgccg tgatcgacgc
     2821 agttgcgcac gaggtgggtc agcgggtcct tcatcgcctc gatcagcgac ttgtcgagtt
     2881 cggtctcctt gccgaccatc tcgacgcgga tctgcttgct gacgccgagc gcgaggtcgc
     2941 gcacgacgcg cggcaccttg ttccagatgt tgttgatcgg ctgcatgcgc gtcttcatga
     3001 cgccttcctg cagctcggtg gtgatcaggt tcagccgctg cgaggtcgcc gcgaagcgcg
     3061 aatcgtcggc ggacttcgag tactgcagca cctggttgcg ggtcagcacc agctcgccga
     3121 cgaggttcat caggtgatcg agcaggccga catcgacgcg gatcgaggtc tcggccaccg
     3181 ccggcgcctt gacctccttc gcgacgtcct tctgcgcctt cagcgcctcg accacggccg
     3241 ccggcttcac ggcctcctcc tgcacgagaa tctcgccgac gtggcgcgga tcgccggcat
     3301 cctgcttcga cagcgcggca tcgacgcgct cgggcgcgac cacgccctgc tgcacgagga
     3361 tctcgccgag ggccggcacc accggcttcg cagccggcgc ggccgccgcc ggggccgcgc
     3421 cgtcgctctg cagcgccgtc agcagctcga tcagctccgg ataggtctcg ttgccgtcgt
     3481 ggccgctcgc accgatcgag ccgagcatca cgcgcacggc atcgaccatc ttcagcagcg
     3541 ccgtcgtgcg ctcctgattc aggcggatgc gaccgtcgcg cagcttcgag agcaggttct
     3601 cgccggcgtg cgtgagcttt tcgagcttcg agaagccgag gaaaccgcag gtgcccttga
     3661 tcgtgtggat ggtgcggaag atgctcgcga ccgtgtcgcg gtcatcgggc gcggcctcga
     3721 gcgcgacgaa ctcgttgtcg aggcggtcga ggttctcctg actctcggtc aggaactcga
     3781 cgacgatctc gtccatgccc tcggccacct cggtcggcac cggcggcggc ggcggcggcg
     3841 ccggggtctt cgccaccggt gccacgaccg cttcctcctt gcgctgcagc gtcagcaggc
     3901 gctcggtgag ttccgggtag ttctggtcgc cgtcgctgcc ggtgctctcg atcgccgcga
     3961 gcatcgcgcg cacggcatcg accatctgca gcagcgccgt cgtgcgctcc tgattcaggc
     4021 ggatgtggcc gtcgcgcagc ttcgagagca ggttctcgcc ggcgtgcgtg accttttcga
     4081 gatgcgaaaa gccgaggaag ccgcaggtgc ccttgatcgt gtggatcgtg cggaagatgc
     4141 tcgcgagcgt gtcccggtcg tcgggactcg actccagcat cacgaactcg ttgtcgagcc
     4201 ggtccaggtt ctcgttactt tccgccagga attcgcggac gatgtcgtca agttcatcca
     4261 tcgctctgtg ttcctcgccg ggtcggcccg ggcaggtgaa tccgctgcgt tgcgcgggag
     4321 cggcgcacgc acgctccggg ggcaccgaaa tagcacaccg gtcaggccgg gccgtagcac
     4381 cggcctgtca agctaggtga attccacaat gcgacaaggg ccggctgcag tccggttccc
     4441 ggagcggcat cccgccccgc cctgcgcaag gcttgccagc agcggcccgc gcccgctaca
     4501 agtcgcgcgc cgaacgggca gcaccggcca gcagccgagg tcgcagcgcc atgcacatca
     4561 tcctggttgc caaccccaag ggcggctccg gcaagaccac gctcgccgtc aacctcgccg
     4621 gctactacgc ggcccacggc cggcgcaccg gcctgttcga tctggaccgt cagcagtcgg
     4681 cgctgcgctg gctggcccgc cggccggcga acctgacgcg catcagcggc ttcgacgccc
     4741 gtgaagacga cacggtgatc gaggacagcg ccggactcga cgtcgccatc atcgatgcac
     4801 cggccgccct gcgcggcgag cgtttcaagg acatcgtccg cattgccgat cacgccatca
     4861 tccccgtgca gccctcggtc ttcgatatcg acgcgagcgc ggcatttctt gagcggctca
     4921 tcgaacagaa atccgtgcgc aaggcgcgct gcctgccggg gctgatcggc aaccgcgtgc
     4981 aggcgcgggc gcgctcggcg cgccagctcg aaaccttcat cgccgggcgc ggcctgccgg
     5041 cgctcggcgc gctgcgcgat tcgcaggtct acgtgcagac ggctgcaagc ggcatcacgc
     5101 tgttcgagct gcccggccgg cgccaggccg ccgatcgggc gagctggcag ccgctgctcg
     5161 actggctggc gataccggcc gccgatccgc cagactgagc caccgtgatg cggaccggat
     5221 ccggatctga tccggacggc tccgcacccg gcccccttgc ccgccccgcc gtccgatccc
     5281 gcccccgcgt cccgtgccga cgcgcccgtc cccgagccgt ggagcccgct ccgatgccag
     5341 gtttcgcccg ttgcctcgtc ctgctgctgt gcctgctgcc ctggacttcg gtcccggcgg
     5401 ccgcggaaac cacgaaacgc ccgccggagc ccccgcgccc gacgctggtc ggactggtgg
     5461 ccggcggcat cggcggcagt tacgcgcggc tggccgatga cctcgccgcc gtgctcgacg
     5521 ccccggacct gcgcatcctg ccgatcctcg ggcgcggctc ggtgcagaac ctgcgcgacc
     5581 tgcaggcgct gccgttcgtc gatctggcgc tggtgcagtc cgacgtgctc gaacacctgc
     5641 gcgcagaccc ggcgcaggag ccgctcgtgc gccggctgca ggtgatcgcc aagctgtaca
     5701 acgaggaatt ccacctgctg gtgcgcggcc acatcacccg cctgagcgac ctgaccggca
     5761 ccaaggtcaa cttcgacgtg ccgggcagcg gcaccgagat caccgccgcc ggcgtctttg
     5821 ccgcgctgaa gctgccggtc gagccgacgc atttcgatca gctcaccgcg ctcgagaaac
     5881 tgcgcagcgg cgagatcagc gcgctcgcct tcgtcgccgg caagcccgca ccgctgttcc
     5941 agcgcgtgcg ccccgaggac gacctgcact tcctgccgat cacgctgccg ccggaactgc
     6001 tcgggcgcta tctgccgacg cgcttcacga aggccgacta tccgaacctg atcgtcggcg
     6061 gcgacgagat ccagaccgtc gccgtcggcg cgctgctggt cacgcaggcg acgcgcaagc
     6121 gcgcgcagct gcgcaagctc gaactgttca ccggccgctt cttcgacagc ttcgccagct
     6181 tccgtcaggc cggccggcat ccgaagtggc gcgaggtcag catcgccgcc caggtcccgg
     6241 actggccgcg gctgaaggcc gccgaggact ggctggcgac gaaccccgac aaggtccgaa
     6301 gctacgccga actcgccgcc cgcgccgccg ccaaggccga ggccgcccgt caggaggccg
     6361 cccgcgccga aaccgcccgc atcgaggccg agaaggccga agccgcccgc aaggccgagg
     6421 tcgcccgcat cgaggccgag aaggccgagg ccgcgcgcaa ggccgagnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc cgaagccgag aaggccgagg cggcgcgcgc
     6601 cgaggccgca cgccgggccg aagccgaaaa ggccgaagcc gcgaaggccg aggccgcgaa
     6661 gcaggccgag gctgcgaagg ccgaggccga caaggctgaa gcggcgcgcg ccgaagccgc
     6721 gaagcaggcc gaggccgcga aggccgaggc cgacaaggcc gaagccgccc gcaaggccga
     6781 aaccgcgaag gccgaggccg acaaggccga agccgcccgc agggccgagg ccgcccgcgc
     6841 cgaagcggcc accaaggccg aggccgcgcg caaggccgat gcggcgaagg ccgaagccgc
     6901 gcgcaaggct gaagccgaaa aggccgaagc gacgaaggcc gaagccgaca agaccgaggc
     6961 cgcccgcaag gccgaggcgg cccgcagcgc cgccgaccgg gcggaagccg aacgcagcgg
     7021 cgccgagtcc ctgccgctga cgccggtgaa tccggccccg gtgacgccct gaaccggcgg
     7081 cgtggcgatc tgcgggcttg aacccgtgcc ggccggcgac gacgatgccg gcatgagccc
     7141 gctcgatcgc ctgccgtttg acaaccgtac cgcccggctg ccggagacgc tgttcgcacg
     7201 cgtcgccgcg cagccgcacg gccgccatca cctggtccac gccagcgccg acgccctggc
     7261 gctgctcgac ctgccgcccg acatcacggc ggacccggac ttcgtcgagg tgttctccgg
     7321 gcggcgcgtg ccggccggcg ccgaaccgat cgcgaccgtc tacgccggtc accagttcgg
     7381 catctgggtg ccgcagctcg gcgacggccg cgcgctgctg ctcggcgagc tcgtcaaccg
     7441 ccgcggcgag cgctgggaag tgcagctcaa gggcgccggg cgcacgccgt actcgcgcat
     7501 gggcgacggc cgcgcggtgc tgcgctcgac gatccgcgaa tacctgggct cggaggcgat
     7561 gcatcacctc ggcatcccga cgacgcgcgc cgccgcgatc atcggcagcg acggcccggt
     7621 gctgcgcgag agcgtcgaga gcggcgcgat cctcgtgcgc ctggccccga gccacctgcg
     7681 cttcgggcac ttccagttcc tgtactacag cggtcgccac gagctgctgc gcgaactgct
     7741 cgacttcgcg atcgcgctgc attttcccgg gctggcggcc ctgcccgacg ccgaacgcca
     7801 cgcggcgctg tttgcgaacg tcatcgagcg cacggcgcag ctcgtcgccg gctggcaggc
     7861 ggtcggcttc acgcacggcg tcctgaacac cgacaacatg agcctgctgg gactgacgat
     7921 cgactacggc ccgttcggct tcctcgacgc ctaccagccc ggcttcgtct gcaaccactc
     7981 cgacgacgac ggccgctacg ccttcgaccg ccagccaagc atcgcgtggt ggaatctgtc
     8041 cgccttcggt cagacgctga cgccgctggt gccggtcgag cggctgcgcg cgctgctcga
     8101 tggcttcagc gagcagtatg cgcaggccta tgccgcgcgc atgcgcgcga agctcggcct
     8161 gcgcgaggcc cgcgacgagg acgaggccct gctcgaagac ctgctcgggc tgatggccgg
     8221 ctgcggcgcc gactacacca acaccttgcg cagcctctgc gatttccggc ccggcaccgg
     8281 cccgcaggcc ctgctcgata ccgtcgccga tccggccggc ttcgagacct ggctgcagcg
     8341 ctacggcgag cgcctgcgcg cggaagcctc cgacgacgag acccgcgccg ccaacatgcg
     8401 gcgtgtcaat ccgaagatcg tgctgcgcaa ctggctcgcg cagcgcgcga tcgaacaggc
     8461 cgagaacggc gactatgccg ggatcgaccg gctgctgcgc ctgctgcaga cgccgttcga
     8521 cgaacacccc gaagccccgg actgctacgg cgagccgccg gactgggcgc gctcgctggc
     8581 gctcagctgt tcgtcctgaa aaacaccctg ccgctcagcc gccgatgcgc aggcgcacga
     8641 acggcaggcg ctgcgcctcg gtctcccaga gctgctcgtg cgtcagcggt cgcagttcac
     8701 cggccgccgg cggcacgccg agcggcgtgt agtcgaccgc gtacggcgtg ccgacaaagc
     8761 ccctgccctc gcgcgccagc cgccagtaca gcccgtcctt gaggtcgatc ccgtattcgc
     8821 tgcgcggctt gatcacgaac agcagcgcgt attcgagatc gaccagatcg ctgtcgcgga
     8881 tgcgccggtg gtgctcgtac ggatacggca gccggcagag cagccactcg ccggccggca
     8941 ggcagcgaaa cggccgcatc gacaggaagt ggtcctcgaa ccgcgactcg tcgaaggcga
     9001 ccgtcaggcg ctcgccgtca ccgtcggctt cgagcgtcac cgtaccgatc acgagcgccg
     9061 ggccctgcgc cggttcgagg ctcaatgcca gctcttcggc cagcaccggg gtgcagggca
     9121 gcacggacag ccagaacgcc acgaggccgg tcgtgcacgc ggtgaagccg ggggaaacgg
     9181 gcatgggcgg agttctccag cagccggcgg atgcagcgga tacgggtccg gacgctcgtg
     9241 cggatgcccg ggccgttcgt gtcgggtcag tcgaggaaga tatccggcag cagcgccgcg
     9301 cccggcgtca cggcgtaggc gtcgaaatcg gtgatgccgg cctcggccag ggcttcctcg
     9361 tcgaggaaga agcggccggt gcagctgcgc gccggccggg tcaggatcgc gtgcgcggca
     9421 tcggcgacga tttcgggttt gcggcagcgg gccggatcga cgccgggcag cagccggatc
     9481 gccgaggtcg cgatgacggt gcgcggccac agcgcgttga ccgccagccc ctgcgcgcgg
     9541 aattcctcgg cgagcccgag cgtcacccag ctcatgccgc ccttggccag cgtgtaggca
     9601 aggtgcgccc cgtaccagcg cgcggcggtg accggcggcg gcgccagcgt caggatgtgc
     9661 ggattgtccg agcgcagcag ctgcgggatg caggcctgcg cgcacaggaa ggtggcgcgg
     9721 gcgttgacgg ccatcatcag gtcgaagcgc ttcatcggcg tgtcgagcgt gccgccgagg
     9781 ctgatcgccg aggcattgtt gaccagcacg tcgagccggc cgaagcggcg cagcgtcgcc
     9841 gccaccgccg caagcacggc ggattcgtcg cgcacgtcga cggcgagcgc cagcgcccgg
     9901 ccgccggcag cctcgatctc ggccgccgtg tcgtggatgg tgccgggcag gcgcggatcg
     9961 ggggtttcgg tcttgccgat cacggcgatg ttcgcgccgt cgcgggcggc gcgcagggcg
    10021 atcgcgcggc cgatgccacg gcttgcaccg gtcacgaaca gcgtgcggcc cgcgagcgtc
    10081 gtcatcgggg tttctcctcg gcccggccgg tcagtcgaag cggtactgca gctgcagtcc
    10141 gaacagcgtc gccgagcccg agtaactgcc gctcagcgaa tcggcgatga cgatggccgg
    10201 gttgccggta tccagcgtca cgacgttgcg cgtgtcggca tcctcgaaga acaggtgcgc
    10261 gaagccggcg tcgatcgtca gcttcggcgt cggccggtag ctggcgccga agccgagcca
    10321 ccagcgatcg gcgtccggga tgcgtgcggt gcggtgttcc tcggaactga ccggcgtctg
    10381 gtcccaggcc acgccggcgc gcagcgtcca ggcctcgctg taacgccagt cgacgccggc
    10441 cgacagcgtc caggtgtcgc gccagtcctc gatgaaggtc ttgctgccgc cggcgaggcc
    10501 cggggacttg agcttcagct catcgaagcg gctccagttg gtccagcgcg ccccaaagga
    10561 caggctcagc tgttcatcga gcgcctgcac gtagccgagg ttgacggtct cgggcagcgt
    10621 gatcgaggtt tcggcatcga agcgacggct gccgagcggt gtgcgcacgc gcaggtcgcc
    10681 gtcgaaatcc tgcgtcacct tcgagcggaa cgacaggccg aggcggctgc ccggcgccag
    10741 ctcgtacatc gcgccgaggt tccagccgaa ggcatcgctg tcgcccttga actcggcaaa
    10801 gccgtcagcc ggcgtgccgg tgaacacggc ctgcgacagc ctgcccttgg cgagctggta
    10861 gctgaggccg aagccgaggc tcagctgcgg cgacagccgc caggccagcg tcggattcag
    10921 gtcgacggtc tgcagttcgg aggactgcgc gtgatagcgc ccgacccagg aagcgccgta
    10981 atcggttgcc agtccgaacg gcgagtgcag gccgaagccg acgcgcaggt ccggcgacag
    11041 cgcatgcacg aagtagttgt tcggcacgac ggccgaggcg ccggcatcct cgctgctgcc
    11101 gccgctgctg tcctgcgaga acaccgggcc ggcgatcggc gcaccggttg cacgcagcga
    11161 cggcgtgccc gtcgagcggc cccggaattc gccgtccgac gaaatggagg tcgcgccgcc
    11221 ctggatgcgg gtgccgtcga gcagcgtcag gccggcaggg ttgtaggcga tggccgacag
    11281 gtcatcgcct accaggccac tgccggcaaa ggcccggccg agcccggtga cgctggattc
    11341 ggtgatctgg aaaccggcgc cgtgggcgac ggtggctgca agggctgcaa gaacggacag
    11401 cccggccgta cgggccggtt tcggcaaggc agatgcggac atcctgatgt tctcccgtgt
    11461 tctcccggat ccgggggtgt catgcaggcg gccttcgggg ccgctctagt gcctgacgtg
    11521 ccgggtaaaa atccgtcccg gccgcagccg gtctagcgta gcagcgccgg cagcgcgacc
    11581 tgcccctgtt gcgatgaagc gacatccgta tcgaatacaa atctcatcgt gaacatccgg
    11641 tgaatctgcc tgcggaaggc gcctgtgtcg cagcgcagca gtggcgcccg gccggcatgc
    11701 gcggtatagt gcgccccacg tgccggcgct cttgtccggc acgccgtgtc atcgctccct
    11761 gacctccccg acgccggcct tgctgcgggc gtacgcgaaa tcccggttgg cgacgatgtc
    11821 gaccgcgccg gcgaaatttc ggccatacca cgcattccac gtgacatctt ccggtccgtg
    11881 ccgccgtgcg cccccgtatt gcaactccgg tagcctaatg tccttttccg acctgtctct
    11941 cgacgcgtcc ctgctgcgtg ccctgaccga agccggctat tccgagccga ccccgatcca
    12001 ggcccaggcc attcccgccg ccctcgaagg gcatgacctc atcgcctccg cgcagaccgg
    12061 caccggcaag accgctgcgt tcatgctgcc ggcgctgcag cgctttgccg tggcctccgg
    12121 tgaccgccgc cgtgcgccgc gcgtgctggt gctgaccccg acccgcgaac tcgccgcgca
    12181 ggtcatggat gccgcgcaca agtacggccg cttcctgcgc ttcacgaccg tcagcctgct
    12241 cggcggcatg ccctacggcc cgcagctgcg cgcgctgggc ggcccgctcg acctggtcgt
    12301 cgccacgccc gggcgcctga tcgatcacct cgaccgcgac cgcatcgacc tgtcgaagat
    12361 cgaggtcctc gtgctcgacg aggccgaccg catgctcgat ctcggcttca aggaagacgt
    12421 cgagcgcatc gccgaggcca cgccggccgg gcgccagacg ctgctgttca ccgcgaccct
    12481 cgacggtgcg atggcgcgcc tcgcggccga gctgacccgc gacgccgtgc gcatcgcgat
    12541 cgagccgacg cagaccgagc agctcaacat cgaacagcgc ctgcacgtcg ccgatgacct
    12601 cgcgcacaag caccgcctgc tcgatcacct gctcaacgac gagacgatgg accgcgcgat
    12661 catcttctcg gcgaccaagc gtgacgccga cgcgctggcc gagcgcctgc gcgatgccgg
    12721 cctgccggcc gcggcgctgc acggcgacat gcaccagacg ctgcgctcgc gcacgctggc
    12781 ccggctacgc gacggtcgcg tgcgcctgct ggtcgccacc gatgtcgccg cgcgcggcat
    12841 cgacgtgcag ggcatcaccc acgtcatcaa cttcgacctg ccgaaggctg ccgaggacta
    12901 cgtgcaccgc atcggccgca ccggccgtgc cgggcgcagc ggcatcgcga tctcgcttgc
    12961 gtcgtacgcc gacgtgcgcc agctcgaacg catcgagcgc tacaccggca agtcgatcga
    13021 ggagcacgtg atcgaagggc tcgaaccgag ccggccgctg cgtcgcggcc gtccgggcgg
    13081 cggcagcggt cgcggtcgcg gcggtccgcg tcgcgaaggc
//
LOCUS       DAOQIE010000066        13028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2334_length_13028_cov_8.180452, whole genome shotgun sequence.
ACCESSION   DAOQIE010000066 DAOQIE010000000
VERSION     DAOQIE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_13028_cov_8.180452"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1859
                     /locus_tag="PK144_05980"
     CDS             138..1859
                     /locus_tag="PK144_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65311.1"
                     /translation="MPAPAAHAAEAEELLLVDDDVDALVELAELLEESGYRCHVAHDA
                     AAARHFLAAQPGIGIALCDLRMPDIDGLTLIGELQQTYPQRLDYVVITGHGDLDDAIA
                     AMRLGVCDFFRKPFDPARLLEQLAALCARRHNARMSAPVLAPAAGRGEDERPAGAAGG
                     ALPELPVPFLPRFLAVLELGSLERLAAALPTRQFMDVLDLAGERLRAVLNDADRLQPL
                     AGGRFLLTTARATPAEIEALYARLVTSLDAPIRADGLELQARLRAGVADCKATAPEAA
                     LARAEQALVQARTLAGGTGLVFHTEALGAQALADLEIESELRQARLRDELELWYQPIV
                     ELENGRLLGFEALLRWQSPRRGLVAPAGFLPALQRSGLAGQVDAWSLVTAATQLARWQ
                     RETGRRDLTLSVNLGCAHLAPGEFDRYCAIARQAVRLPAPGTLRIEITEDGPGPQREP
                     YLDGLRALHLAGLRFYLDDYGTGEAALSRLNGLNFEAIKLDRSFVLAPTPENLAICET
                     VIALGRRLGLAVIAEGIEDAAALERLLGFGCRAGQGYWFSPPVPADLAALMLRSQTLT
                     PRVPKRA"
     gene            1893..2264
                     /locus_tag="PK144_05985"
     CDS             1893..2264
                     /locus_tag="PK144_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65312.1"
                     /translation="MLLLIDDEPDILAEIQEWFEWQGWDCLCAGRLAEAERLLAGRDD
                     IRLVVTDLKLVDGEAFGLIEARLARPPAPDFIIVTGHAGEPEIEHARRIGVQLLHKPL
                     DLAALDAAVAATGHLPRGTPS"
     gene            2261..4363
                     /locus_tag="PK144_05990"
     CDS             2261..4363
                     /locus_tag="PK144_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF65313.1"
                     /translation="MTMIRRMQDRRLLTLYFAGAFLVLLVAGLLAGVALKTDYQRRMD
                     GAERDSQNLARAFEEHIYRTFRSLDQQSRFLAEYYFRYPDKDELTEALRGLLRIEDAA
                     VQHAIIDEHGILAGSSVGTQSLGINLSDRAHFRHYLQPDATPTYVSKPLVGRASGKAS
                     IQYTRRIEDSDGEFRGVAVVSFDPLYLNSFYQSVDVGERGVISLAGTDGILRARSIDM
                     DQTIGKDISGGELMRSYQSAPAGTYRAPSGVDGIERIVSYRKVRDFPLLVVVGLGLGD
                     VMAGYTGYVASVGGLYGFLLVTLVLLGGVLRNFLRNRTEAERLARETVVERRERQFLS
                     SILQTAGVLVVVAEADGTIKVANPLFARYFGAGSGARAWSRAATGEEFAALVTGLPRS
                     FEVQVSDLGGIRRTVAWTFTAVTDDGGELRNLVGIGLDNTERRAAELMIYQSAKLVTL
                     GEMATGLAHELNQPLNAIKLTVENVLLRSSRGTPEKTYLDDKLDKIRRNVARAASIID
                     HMRIFGRRAERTLGPVDPAQACEGALSITNAPLRLANIACHVICPAGMYWVRAEIGLL
                     EQVLINLILNARDAILSRREQHPETGRADHIELSAQPLADGRMQISVTDTGGGVPEAL
                     MQRIFEPFFTTKPVGQGTGLGLSLSYGIVRDFGGELRVENIEGGACFLVELPAATPAT
                     GPAALPVPAPQAQAQAAQ"
     gene            4517..8179
                     /locus_tag="PK144_05995"
     CDS             4517..8179
                     /locus_tag="PK144_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNF65314.1"
                     /translation="MNPPAHSAPPVSGWQFWIDRGGTFTDLVARSPDGRLLATKLLSE
                     HPERYRDAAVEGIRRLLGLAAGAPLPAGAIEVVKMGTTVATNALLERRGEPVLLVITR
                     GHADALRIAYQNRPQLFARHIVLPAPLYAEVLEADERLAADGSVLKPLDEIALARGFA
                     AARARGLRACAIVFLHGYREPRHERRAAALARIAGFTQVSVSHEVSPLMKLVGRGDTT
                     VADAYLSPVLRRYVGQVAEALGDVRLQFMQSNGGLADAAHFRGKDAVLSGPAGGVVGM
                     VETARLAGFGCVIGFDMGGTSTDVSHCAGEYERVWDTTVAGVRLRVPMLAIHTVAAGG
                     GSIVRFDGTRLRVGPESAGADPGPAAYRRGGPLTVTDCNVALGRLQPVHFPALFGPHG
                     NQPLDTGAVAAGFERLAGEIEAATGTRRPVEALAAGALAIAVEHMAAAIRRISVERGH
                     DLAAYALACFGGAGGQHACRVAEALGMRTVFIHPLAGVLSAYGMGLADCRVLRQRAIE
                     AELDAALADALFRCAGELAVQARTALAEQGIPAAAIRIEQRVRLRYRGTDTALSVALA
                     EPAAMAAAFAEAHRRQFGFTLAGRPLTVEAVDVEALGRAHMPVEPERPRPADAAGAPV
                     PLAHVRAWFGDWRRVPVFGLAALHAGDAVAGPAILLDPHATTVVEPGWQGRVTARGHL
                     LLEHVGIAGRAAAGTALDPVRLEIFNRRFMAIAEQAGAVLAQTAVSVNIKERLDFSCA
                     LFDAGGGLVANAPHMPVHLGSMAASVQSVIRRHAASVRPGMAYALNDPYRGGTHLPDL
                     TVIAPVFVEDDEDDDGGPVCWVANRGHHADIGGRTPGSMPPDSVTLAEEGVLFSGECI
                     VADGELLEADIRERLAAGPWPARNPEQNLADLRAQLAANETAAAAVRALIAQTGLPAV
                     RAYMGHVQANAEAAVRRVLATLEGGSCRVETDDGSVICVRIDVDRAARTARIDFRGTS
                     VQGAHNRNAPQAITLAAVLYVFRTLVDDDIPLNQGCLAPLDILIPEGSLLQPRPPAAV
                     VAGNVETSQAIVDALFGALGTQAAAQGTMNNLTFGDEQLQYYETICGGSGAGPGFDGT
                     DAVHTHMTNSRLTDPELLEWRYPVLVEDFCIRRGSGGAGRWRGGDGVVRRLRFRAPMT
                     AAILSDHRRVPPQGMAGGGPGACGINRIERADGRIERLGGIATVSMQPGDAFVIETPG
                     GGGYGAPDPSGPTSCG"
     gene            8214..9581
                     /locus_tag="PK144_06000"
     CDS             8214..9581
                     /locus_tag="PK144_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF65315.1"
                     /translation="MSDELIARARRYATEAHARIDQRRKYTGQPYEVHLKAVAALILR
                     HGGDAAMVAAAWLHDTVEDTPATLGDLEREFGADVAALVAQLTDVSRPADGNRAVRRA
                     IDRAHSAQASARAQTIKLADLIDNCRDLVRHDPAFARTFTAEMALLLDVLVEADPKLR
                     QQARRTLERAQAELERLEHAPPPAPSPPPAPAETERLRRQSRIYAHAFSAQDIAEALP
                     SFDGERPAAAVAASLDAGGHGVAGVRQEGRIVASVKRAALDALGEAACAQAARALTRA
                     ECVASEAGLAEVVHVLTRHDHAFVTLLGEPVAVIGRNDMQKPVVRMWLFGILTLAELD
                     FTGRIRARWPGGEWTAKLSAARLDKARQLQLERQRRGHACTLLDCLQFGDKLRLLIED
                     PAELARLDMPGRSAAKRVIADLESLRNHLAHAQDIVSHDWAQIARLARRIEEMSSGTG
                     LPEGG"
     gene            9636..9989
                     /locus_tag="PK144_06005"
     CDS             9636..9989
                     /locus_tag="PK144_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003198223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF65316.1"
                     /translation="MQPLLGVRFFRSAAGTEPVREWLRDLPVEHRRAIGTDIKTVQYG
                     WPLGMPLVRKLEPGLWELRSHIADGIARVLFTVHGADLVLLHGFIKKSGRIPVDELDT
                     ARQRLALFSRWADRD"
     gene            9928..10266
                     /locus_tag="PK144_06010"
     CDS             9928..10266
                     /locus_tag="PK144_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF65317.1"
                     /translation="MNSTPLASVWRCFQGGQTVTDHPHIGSDFDDFLAEEGLLESAGA
                     LAIKRVVAWQIREAMKAQKLSKKAMAERMGTSRAALDRVLDENDPGMTLATLARAARA
                     LGKRVEIKVS"
     gene            complement(10327..11058)
                     /locus_tag="PK144_06015"
     CDS             complement(10327..11058)
                     /locus_tag="PK144_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF65318.1"
                     /translation="MEILLDPQTWLSLLTLTALEIVLGIDNLVFISILSSRLPAHQQS
                     TARRVGLALAVITRLLLLLSISWLAGVTAPLFSVFDHPVSLRDLILLGGGLFLLAKGT
                     TEIHATVEGVEEEIRESKAVSFTSVVVQIMILDIVFSLDSVITAVGMASEISVMAAAV
                     IIAVLVMVFAADPLSRFIEEHVSVKMLALSFLILVGVALIADGMGFHIPKGYLYFAIA
                     FSVGVETLNILAKKKRMKAKAEAAH"
     gene            11314..11916
                     /locus_tag="PK144_06020"
     CDS             11314..11916
                     /locus_tag="PK144_06020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNF65319.1"
                     /translation="MSVLVTREAPDFTAAAVLADGSIVEDFSLSSLRGKYVVLFFYPL
                     DFTFVCPSEIIAHGNRVAKFKELGVEVVGVSIDSQFTHFAWRETPVEKGGIGRVPFTL
                     VADVKHDICRAYGVEHPGAGVALRGSFLIDKDGVVQAQQVNNLPLGRNVDEMVRLVEA
                     LQFTEEYGEVCPAGWNKGDKGMKPTADGVAAYLAEEATAL"
     gene            12088..>13028
                     /gene="trxB"
                     /locus_tag="PK144_06025"
     CDS             12088..>13028
                     /gene="trxB"
                     /locus_tag="PK144_06025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNF65320.1"
                     /translation="MSETRHSRLLILGSGPAGYTAAVYAARANLKPVLITGLQMGGQL
                     TTTTEVDNWPGDVEGLMGPALMERMQRHAERFNTEIVFDHIHTAKLGERPFTLIGDSG
                     TYTCDALIIATGASAQYLGLPSEEAFMGKGVSACATCDGFFYRNKPVAVVGGGNTAVE
                     EALYLANIASHVTLIHRRDKFRAEAILIDHLHEKVEAGKITLATSATLDEVLGDAGGV
                     TGARLKSTTDGSTRDIGVDGVFIAIGHKPNTDLFSGQLEMHNGYLVVQSGLAGNATQT
                     SIPGVFAAGDVADHIYRQAVTSAGTGCMAALDAERWL"
BASE COUNT         1900 a         4738 c         4543 g         1847 t
ORIGIN      
        1 gctgcaactg taatagtctg caacgaccgg tgcgcgtgta acgtcgttca cggtcgtgtg
       61 ccgtgccagc cggcaagata ccgcccttgc gcgcccggcg gtcccgccgg agtgattttc
      121 gtccacggag gctccgtatg cctgctcccg ccgcccatgc cgcagaggca gaggaactgc
      181 tgctcgtcga tgacgatgtc gatgcgctgg tcgagctggc cgagctgctg gaggaaagcg
      241 gctaccgctg tcacgtcgcc cacgatgcgg ccgccgcccg gcatttcctc gccgcgcaac
      301 ccggcatcgg catcgcgctc tgcgatctgc gcatgcccga catcgacggc ctgacgctga
      361 tcggcgaact gcagcagacc tatccgcagc ggctcgatta cgtcgtgatc accggccacg
      421 gcgatctcga cgatgcgatc gcggcgatgc gtctgggcgt gtgtgacttc ttccgcaagc
      481 ccttcgatcc ggcccggctg ctcgaacagc tcgccgcgct gtgcgcacgc cggcacaacg
      541 cccggatgtc ggcgccggtg ctggcaccgg ccgccggccg cggcgaggac gagcgccctg
      601 ccggggccgc cggcggcgcg ctgccggagc tgccggtgcc gttcctgccg cgctttctgg
      661 ccgtgctcga actcggcagc ctggagcggc tggccgcggc gctgccgacc cggcagttca
      721 tggacgtgct cgatctggcc ggcgaacgcc tgcgcgcggt gctgaacgat gccgatcgcc
      781 tgcagccgct ggccggcggc cgcttcctgc tgaccacggc gcgtgccacc ccggccgaaa
      841 tcgaggcgct gtacgcgcgc ctggtcacca gcctcgatgc gccgatccgc gccgacggcc
      901 tcgaactgca ggcgcggctg cgcgccggcg tcgccgactg caaggccacg gcgcccgagg
      961 ccgcgctcgc gcgtgccgag caggcgctgg tgcaggcgcg cacgctggcg ggcggcaccg
     1021 ggctggtgtt ccacaccgag gcgctcggcg cgcaggcgct cgccgatctg gagatcgaga
     1081 gcgagctgcg acaggcgcgg ctgcgcgacg agcttgagct ctggtaccag ccgatcgtcg
     1141 agctggagaa cggccggctg ctcggcttcg aggcgctgct gcgctggcag agtccgcgcc
     1201 gcgggctggt ggcaccggcc ggcttcctgc cggcgctgca gcgcagcggc ctcgccggac
     1261 aggtcgatgc ctggtcgctg gtcacggccg cgacccagct cgcccgctgg cagcgcgaga
     1321 ccggccggcg cgacctgacg ctgagcgtca acctcggctg cgcccacctc gcgcccggcg
     1381 agttcgatcg ctactgcgcg atcgcccgtc aggccgtgcg cctgccggca ccggggacgc
     1441 tgcgcatcga gatcaccgag gacggccccg gcccgcagcg cgaaccgtac ctcgacggcc
     1501 tgcgcgcgct gcacctggcc ggcctgcgct tctacctcga cgactacggc accggcgagg
     1561 cggcactgtc gcggctcaac ggcctgaact tcgaagcgat caagctcgat cgcagcttcg
     1621 tgctggcgcc gacgccggag aacctcgcga tctgcgagac cgtcatcgcc ctcgggcgcc
     1681 ggctcggcct cgcggtgatc gccgagggca tcgaggatgc cgctgcgctc gaacgcctgc
     1741 tcggtttcgg ctgccgcgcc ggccagggct actggttctc gccgccggtg ccggccgatc
     1801 tggcggcgct gatgctgcgc tcgcagacgc tgaccccgcg cgtgccgaag cgtgcctgag
     1861 cgcgcccgca acgcccgcaa ggagcaccga acatgctgct gctgatcgat gacgaacccg
     1921 acatcctcgc cgagatccag gaatggttcg aatggcaggg ctgggactgc ctgtgcgccg
     1981 ggcgcctcgc cgaggccgag cgcctgctcg ccgggcgcga cgacatccgg ctggtggtca
     2041 ccgacctgaa gctggtcgac ggcgaggcct tcgggctgat cgaggcgcgg ctcgcgcgcc
     2101 cgccggcgcc ggacttcatc atcgtcaccg gccacgccgg tgaacccgaa atcgaacacg
     2161 cacgccgcat cggcgtgcag ttgctgcaca agccgctcga tctggccgcg ctcgacgccg
     2221 cggtcgccgc gaccggccat ctgccgcggg gaacgccgtc atgaccatga tccgtcgcat
     2281 gcaggaccgc cgcctgctga cgctgtactt cgccggcgcg ttcctggtgc tgctggtcgc
     2341 cggcctgctc gccggcgtgg cgctgaagac cgactaccag cggcgcatgg acggggccga
     2401 gcgcgacagc cagaacctgg cgcgcgcctt cgaggagcac atctaccgga ccttccgctc
     2461 gctcgaccag cagagccgct tcctcgccga gtactacttc cgctacccgg acaaggatga
     2521 gctgaccgag gcgctgcgcg ggctgctgcg catcgaggat gccgccgtgc agcacgcgat
     2581 catcgacgag cacggcatcc tcgccggttc cagcgtcggc acgcagtcgc tcggcatcaa
     2641 tctttcggac cgcgcgcatt tccgccatta cctgcagccc gacgcgacac cgacctacgt
     2701 cagcaagccg ctggtcggac gcgccagcgg caaggcctcg atccagtaca cgcggcgcat
     2761 cgaggacagc gacggcgagt tccgcggcgt cgcggtcgtg tccttcgatc cgctgtacct
     2821 gaacagcttc tatcagtcgg tcgacgtcgg cgagcgcggc gtcatctcgc tggccggcac
     2881 cgacggcatc ctgcgcgcgc gctcgatcga catggaccag accatcggca aggacatctc
     2941 cggcggcgag ctgatgcgca gctaccagag cgcaccggcc ggcacctacc gcgcgccgag
     3001 cggcgtcgac ggcatcgagc gcatcgtcag ctaccgcaag gtccgtgact tcccgctgct
     3061 cgtcgtcgtc ggcctcggcc tcggcgacgt gatggccggc tacaccggct acgtggcctc
     3121 ggtcggcggc ctgtacggct tcctgctggt gacgctggtg ctgctcggcg gcgtgctgcg
     3181 caacttcctg cgcaaccgca ccgaggccga gcgtctggcg cgcgagaccg tggtcgagcg
     3241 ccgcgagcgc cagttcctca gctcgatcct gcagaccgcc ggcgtgctgg tcgtcgtcgc
     3301 cgaggccgac ggcacgatca aggtcgccaa cccgctgttc gcgcgctact tcggcgccgg
     3361 cagcggcgcg cgcgcctgga gccgtgccgc caccggcgag gaattcgcgg cgctggtcac
     3421 cggcctgccg cgcagcttcg aggtgcaggt cagcgatctc ggcggcatcc ggcgcacggt
     3481 ggcctggacc ttcacggcgg tcaccgacga cggcggcgag ctgcgcaacc tcgtcggcat
     3541 cggcctcgac aacaccgagc ggcgcgccgc cgagctgatg atctaccagt cggcgaagct
     3601 ggtgacgctc ggcgagatgg ccaccggcct cgcgcacgag ctgaaccagc cgctgaacgc
     3661 gatcaagctg accgtcgaga acgtgctgct gcgctccagc cgcggcacgc cggagaagac
     3721 ctatctcgac gacaagctcg acaagatccg ccgcaacgtc gcgcgcgccg cgtcgatcat
     3781 cgaccacatg cgcatcttcg gccgccgcgc cgaacgcacg ctcggcccgg tcgatccggc
     3841 ccaggcctgc gaaggggcgc tgtcgatcac caacgcgccg ctgcgcctcg ccaacatcgc
     3901 ctgccacgtg atctgtccgg ccggcatgta ctgggtgcgc gccgagatcg gcctgctcga
     3961 acaggtgctg atcaacctga tcctgaacgc ccgcgacgcg atcctgagcc ggcgcgagca
     4021 gcatccggaa accggccgcg ccgatcacat cgagctgagc gcgcagccgc tcgccgacgg
     4081 ccgcatgcag atcagcgtca ccgacaccgg cggcggcgtg cccgaggcgc tgatgcagcg
     4141 catcttcgag cccttcttca cgaccaagcc ggtcggacag gggaccgggc tcgggctgtc
     4201 gctgtcctac ggcatcgtcc gtgatttcgg cggcgaactg cgcgtcgaga acatcgaggg
     4261 cggtgcatgc ttcctggtcg agctgccggc cgcgaccccg gccaccggcc cggccgcgct
     4321 gccggtgccg gcaccacagg cacaggcgca ggcggcgcag taagggggct ggctgcccgc
     4381 gcggccccgt ccggaggtcc gccgccgctg cggcggcgac gcgcgaagca ctgccggcag
     4441 cggcgacagc ggcaatcgtc gccggtgcga ctggtatcct cgcggcctgc acccgtccga
     4501 gccggaggcc gccgtcgtga acccgcccgc ccatagcgcg ccgcccgtga gcggctggca
     4561 gttctggatc gaccgcggtg gcaccttcac cgatctggtc gcccgcagcc ccgatggccg
     4621 gctgctcgcc accaagctgc tgtccgaaca cccggagcgc taccgcgatg ccgccgtcga
     4681 gggtatccgc cgcctgctcg gtctcgccgc cggtgcgccg ctgccggccg gggcgatcga
     4741 ggtcgtgaag atgggcacga cggtcgcgac caacgcgctg ctggagcgcc gaggcgagcc
     4801 ggtgctgctg gtgatcacgc gcggccacgc cgatgcgctg cgcatcgcct accagaaccg
     4861 tccgcagctg ttcgcgcgcc acatcgtgct gcccgcgccg ctgtatgccg aggtgctcga
     4921 agccgacgag cggctggccg ccgacggctc ggtgctgaag ccgctcgacg agattgcgct
     4981 cgcgcgcggc ttcgccgcag cgcgcgcccg cggcctgcgc gcctgcgcga tcgtgttcct
     5041 gcacggctat cgcgaaccgc ggcacgaacg ccgcgccgcg gcgctggcgc gcatcgccgg
     5101 cttcacgcag gtgtcggtgt cgcacgaggt cagcccgctg atgaagctgg tcgggcgcgg
     5161 cgacaccacg gtcgccgacg cctacctgtc gccggtgctg cgccgctacg tcggccaggt
     5221 ggccgaggcg ctcggcgacg tgcgcctgca gttcatgcag tccaacggtg gcctcgccga
     5281 tgccgcgcat ttccgcggca aggacgccgt gctctccggc cccgccggcg gcgtggtcgg
     5341 catggtcgag accgcgcgcc tcgccggctt cggctgcgtg atcggcttcg acatgggcgg
     5401 cacctcgacc gatgtctcgc actgcgccgg cgaatacgag cgcgtctggg acaccacggt
     5461 cgccggcgtg cgcctgcgcg tgccgatgct ggcgatccac accgtcgccg ccggcggcgg
     5521 ctcgatcgtg cgcttcgacg gcacgcgcct gcgcgtcggc ccggagtcgg ccggcgccga
     5581 tccggggccg gccgcctacc ggcgcggcgg accgctgacc gtcaccgact gcaacgtcgc
     5641 gctcgggcgg ctgcagccgg tgcatttccc ggcgctgttc gggccgcacg ggaaccagcc
     5701 gctcgatacc ggtgcggtcg ccgccggctt cgagcgcctc gccggcgaga tcgaggcggc
     5761 caccggcacg cgccggccgg tcgaggcact cgccgccggg gcgctcgcga tcgccgtcga
     5821 gcacatggcg gcggcgatcc ggcgcatctc ggtcgagcgc ggtcatgatc tcgccgccta
     5881 cgcgctcgcc tgcttcggcg gggccggcgg ccagcacgcc tgccgggtcg ccgaggcgct
     5941 cggcatgcgc acggtgttca tccatccgct cgccggcgtg ctgtcggcct acggcatggg
     6001 actcgccgac tgccgcgtgc tgcgccagcg cgcgatcgag gccgagctcg atgcggcact
     6061 ggccgatgcg ctgttccgct gcgccggcga actggccgtg caggcgcgca cggcgctcgc
     6121 cgagcagggc attccggccg cggcgatccg gatcgagcag cgcgtgcgcc tgcgctaccg
     6181 cggcaccgac acggcgctga gcgtcgcgct cgccgagccg gcagcgatgg ctgcggcctt
     6241 cgccgaggcg caccgccggc agttcggctt cacgctcgcc gggcggccgc tgacggtcga
     6301 ggccgtcgat gtcgaggcgc tcgggcgcgc gcacatgccg gtcgaacccg agcgcccgcg
     6361 cccggccgat gccgcggggg caccggtgcc gctcgcgcac gtgcgcgcct ggttcggcga
     6421 ctggcggcgg gtgccggtgt tcgggctggc ggcgctgcac gccggcgatg ccgtcgccgg
     6481 gccggcgatc ctgctcgatc cgcacgcgac cacggtcgtc gagccgggct ggcaggggcg
     6541 ggtgacggcg cgcgggcatc tgctgctcga acacgtcggc atcgccgggc gcgcagcggc
     6601 cgggaccgcg ctcgatccgg tgcgcctcga aatcttcaac cgccgcttca tggccatcgc
     6661 cgagcaggcc ggcgccgtgc tcgcgcagac ggcggtgtcg gtcaacatca aggagcggct
     6721 ggatttctcg tgcgcgctgt tcgacgccgg cggcgggctg gtcgccaacg ccccgcacat
     6781 gccggtgcat ctgggctcga tggccgcgag cgtgcagagc gtgatccgcc gccacgccgc
     6841 cagcgtgcgt ccgggcatgg cctacgcgct caacgacccc taccgcggcg gcacgcacct
     6901 gccggacctg accgtcatcg cgccggtatt cgtcgaggac gacgaggatg acgacggcgg
     6961 gccggtgtgc tgggttgcca accgcggcca tcacgccgac atcggcggac gcacgcccgg
     7021 ctcgatgccg ccggactcgg tgacgctcgc cgaggagggc gtgctgttct cgggcgagtg
     7081 catcgtcgcc gacggcgagc tgctggaggc cgacatccgc gagcgcctcg ccgccggacc
     7141 gtggccggca cgcaatcccg agcagaacct cgccgacctg cgcgcgcagc tcgccgccaa
     7201 cgaaaccgcc gccgcggccg tgcgcgcact gatcgcgcag accggcctgc cggccgtgcg
     7261 cgcctacatg ggccacgtgc aggccaacgc cgaggccgcg gtgcgccgcg tgctggcgac
     7321 gctcgaaggc ggcagctgcc gcgtcgagac cgacgacggc tcggtgatct gcgtgcgcat
     7381 cgacgtggat cgcgcggcgc gcacggcgcg catcgacttc cgcggcacca gcgtccaggg
     7441 cgcgcacaac cgcaacgccc cgcaggcgat cacgctcgcc gcggtgctgt acgtgttccg
     7501 cacgctggtc gatgacgaca tcccgctgaa tcagggctgt ctcgcgccgc tcgacatcct
     7561 gattcccgaa ggctcgctgc tgcagccgcg cccgccggcg gcggtggtcg ccggcaacgt
     7621 cgagacctcg caggcaatcg tcgatgcgct gttcggcgcc ctcggcacgc aggccgcggc
     7681 gcagggcacg atgaacaacc tgaccttcgg cgacgagcag ctgcagtact acgagacgat
     7741 ctgcggcggc agcggcgccg gccccggctt cgacggcacc gacgccgtgc acacgcacat
     7801 gaccaactcg cgcctgaccg atccggagct gctcgaatgg cgctatccgg tgctggtcga
     7861 ggacttctgc atccgccgcg gctcgggcgg cgccgggcgc tggcgcggcg gcgacggcgt
     7921 cgtgcgccgg ctgcgcttcc gggcgccgat gacggcggcg atcctgtcgg atcatcggcg
     7981 cgtgccgccg caaggcatgg ccggcggtgg tcccggcgcc tgcggcatca accgcatcga
     8041 gcgcgccgac ggccgtatcg agcggctcgg cggcatcgcc acggtgtcga tgcagcccgg
     8101 cgatgccttc gtgatcgaga caccgggcgg cggcggctac ggcgcgccgg acccgtccgg
     8161 gcccacgtcc tgcggatgag cagggttcgt ccgtttccgg agccgccagc gccatgtccg
     8221 acgaactgat cgcccgcgcc cgccgctacg ccaccgaggc gcacgcgcgc atcgaccagc
     8281 ggcgcaagta caccggccag ccctacgagg tgcacctgaa ggcggtcgcg gcgctgatcc
     8341 tgcgccacgg cggcgatgcg gcgatggtcg ccgccgcctg gctgcacgat acggtcgagg
     8401 acacgccggc gacgctcggt gacctcgaac gcgagttcgg cgccgatgtc gccgcgctgg
     8461 tcgcgcagct gaccgacgtg agccgcccgg ccgatggcaa ccgcgccgtg cgccgcgcca
     8521 tcgaccgggc gcacagcgcg caggcttcgg cgcgcgcgca gaccatcaag ctcgccgacc
     8581 tgatcgacaa ctgccgcgat ctggtgcgcc acgacccggc cttcgcccgc accttcacgg
     8641 ccgagatggc gctgctgctc gacgtgctgg tcgaggccga cccgaagctg cgccagcagg
     8701 cccggcgcac gctggagcgg gcgcaggccg agctcgaacg gcttgagcac gcgccgccgc
     8761 cggctccaag cccgccgccc gcaccggccg agaccgaacg cctgcgccgg cagagccgca
     8821 tctacgcgca tgcgttctcg gcgcaggaca tcgccgaggc gctgccgtcc ttcgacggcg
     8881 aacgcccggc cgccgcggtc gctgccagcc tcgatgccgg cggccacggt gtggccggcg
     8941 tgcgtcagga gggccgcatc gtcgccagcg tgaagcgcgc cgcgctcgat gcgctcggcg
     9001 aggccgcctg tgcgcaggcg gcgcgggcgc tgacgcgcgc cgaatgcgtg gccagcgagg
     9061 ccggactcgc cgaggtcgtg cacgtgctga cccgccacga ccacgccttc gtgacgctgc
     9121 tcggcgagcc ggtcgcggtc atcgggcgca acgacatgca gaagccggtc gtgcgcatgt
     9181 ggctgttcgg catcctgacg ctcgccgaac tcgacttcac cggccgcatc cgcgcccgct
     9241 ggccgggcgg cgagtggacg gcgaagctgt ccgccgcgcg cctcgacaag gcgaggcagc
     9301 tgcagctcga acgccagcgc cgcggccacg cctgcacgct gctcgactgc ctgcagttcg
     9361 gcgacaagct gcgcctgctg atcgaggacc cggccgaact cgcccgcctc gacatgcccg
     9421 gacgcagcgc cgcgaagcgc gtgatcgccg acctcgaatc gctgcgcaac cacctcgcac
     9481 acgcgcagga catcgtcagt cacgactggg cgcagatcgc gcggctggcg cggcggatcg
     9541 aggagatgag cagcgggacg gggctgccgg agggagggtg acgaggctga gggcggcatg
     9601 tctgcctgtg catcaaaaaa tgtacactcc cggccatgca gcccttgctc ggtgtccggt
     9661 tctttcgcag cgcggccggt accgagccgg tccgggaatg gttgcgggat ttgcccgtcg
     9721 agcatcgcag ggccatcggc accgacatca agaccgtgca gtacggctgg ccgctcggca
     9781 tgccgctggt gcgcaagctc gaacccggac tctgggaact gcgttcgcac atcgccgacg
     9841 gtattgcgag agtgctgttc accgtgcatg gggccgatct ggtgctgctg catggcttca
     9901 tcaagaaatc cggcaggatt ccggtcgatg aactcgacac cgctcgccag cgtctggcgc
     9961 tgttttcaag gtgggcagac cgtgactgat catccgcaca tcggcagcga tttcgatgat
    10021 ttcctggccg aggaaggcct gctggaaagc gcgggagcgc tggccatcaa gcgcgtggtc
    10081 gcctggcaga tccgggaagc catgaaggcc cagaagctca gcaagaaggc aatggccgag
    10141 cgcatgggga cgagccgggc ggcgctcgac cgggtgctgg acgaaaacga tcccggcatg
    10201 accctcgcca cgcttgcccg tgctgcccgg gcgctcggca agcgggtcga gatcaaggtt
    10261 tcctgaagcg gtcgcttcag gccggatgcc tgcccgccag agcaggcatc cggtttctga
    10321 accccgtcag tgcgccgctt cggccttcgc cttcatccgc ttcttcttcg cgaggatgtt
    10381 cagcgtctcg acgccgaccg agaacgcgat cgcgaagtac aggtagccct tcgggatgtg
    10441 gaagcccatg ccgtcggcga tcagcgcgac gccgaccagg atcaggaagg acagcgccag
    10501 catcttcacc gacacgtgct cctcgatgaa gcgcgacagc gggtcggccg cgaacaccat
    10561 gaccagcacc gcgatgatga ccgccgcggc catcaccgaa atctcgctgg ccatgccgac
    10621 cgccgtgatc accgagtcga gcgagaacac gatgtcgagg atcatgatct gcacgaccac
    10681 cgacgtgaag ctgaccgcct tcgactcgcg gatttcctcc tcgacgccct cgacggtggc
    10741 gtggatttcg gtcgtgccct tggcgagcag gaacaggccg ccgccgagca ggatcaggtc
    10801 gcgcaggctc accgggtgat cgaacaccga gaacagcggc gcggtgacgc cggccagcca
    10861 cgagatcgac agcagcagca gcaggcgcgt gatcacggcc agcgccagac cgacgcgccg
    10921 ggccgtgctc tgctgatgcg cgggcaggcg cgaggacagg atcgagatga acaccaggtt
    10981 gtcgatgccg agcacgatct cgagcgcggt cagcgtcagc aggctcagcc aggtttgcgg
    11041 gtccagcagg atttccatgg atgggctact cgtcggagga ggcgggcgaa ggcgcgggga
    11101 aggcgcggga aaagggcatg agcggggctg cgaagcgcga gtatagaatc cggcggctgc
    11161 cttacaggtg atgattcgtt gagcgcgctg ataggcacgc gctatcgtcg tgatcggaaa
    11221 ttttcaattc ccctaccggt tcgcggcaag gctatgctgc gccgcgtcta cgacttccgt
    11281 agtccttaca aggccagcca aggagatcca ttcatgagcg tactcgtcac ccgcgaagcc
    11341 ccggacttca ccgccgctgc cgtgctcgcc gacggcagca tcgtcgagga cttctcgctc
    11401 tccagcctgc gcggcaagta cgtggtgctg ttcttctacc cgctcgactt caccttcgtc
    11461 tgcccgtccg agatcatcgc ccacggcaac cgtgtcgcga agttcaagga actcggcgtc
    11521 gaggtggtcg gcgtgtcgat cgactcgcag ttcacgcact tcgcgtggcg cgagacgccg
    11581 gtcgagaagg gcggcatcgg ccgcgtgccg ttcacgctgg tcgctgacgt caagcacgac
    11641 atctgccgcg cctacggcgt cgagcacccg ggcgccggcg tcgcgctgcg cggctccttc
    11701 ctgatcgaca aggatggcgt cgtgcaggcc cagcaggtca acaacctgcc gctcgggcgc
    11761 aacgtcgacg agatggtgcg tctggtcgag gcgctgcagt tcaccgagga atacggcgaa
    11821 gtctgcccgg ccggctggaa caagggcgac aagggcatga agccgaccgc cgatggcgtc
    11881 gccgcctatc tggccgagga agccacggcc ctgtaaggtc gcacgccgca ccggcagggg
    11941 gcttgccccc cgccggtgtc gagccgagag cgcatgccgg ggcgtgtgcg ctgtcgcatc
    12001 gacacccttg agcaggccgt atccgacgga gcgggcgccg cagcgtccgc gcccgatccc
    12061 gaactgaatc cccggagttc ccccgcgatg agcgaaaccc gccattcccg cctgctgatc
    12121 ctcggttccg gaccggccgg ctacacggcc gccgtctatg cggcgcgcgc caacctcaag
    12181 cccgtgctga tcaccggcct gcagatgggc ggccagctga cgacgacgac cgaggtcgac
    12241 aactggcccg gcgatgtcga gggcctgatg gggccggcgc tgatggagcg catgcagcgc
    12301 cacgccgagc gcttcaacac cgagatcgtg ttcgatcaca tccacaccgc gaagctcggc
    12361 gagcggccgt tcacgctgat cggcgattcc ggcacctaca cctgtgatgc gctgatcatc
    12421 gccaccggcg ccagcgccca gtacctcggc ctgccgtccg aggaagcctt catgggcaag
    12481 ggcgtgtcgg cctgcgcgac ctgcgacggc ttcttctacc gcaacaagcc ggtcgccgtg
    12541 gtcggcggcg gcaacaccgc ggtcgaggag gcgctgtacc tggccaacat cgcctcgcac
    12601 gtgacgctga tccaccgccg cgacaagttc cgcgccgagg cgatcctgat cgatcacctg
    12661 cacgagaagg tcgaggccgg caagatcacg ctcgcgacca gtgccacgct cgatgaggtg
    12721 ctcggtgacg ccggcggcgt caccggcgcg cgcctgaaga gcacgaccga cggctcgacg
    12781 cgcgacatcg gcgtcgatgg cgtgttcatc gccatcggcc acaagccgaa caccgacctg
    12841 ttcagcggcc agctggagat gcacaacggc tatctggtcg tgcagtccgg tctggccggc
    12901 aacgccacgc agaccagcat ccccggcgtg ttcgccgccg gcgacgtggc tgatcacatc
    12961 taccgccagg ccgtgacctc ggccggcacc ggctgcatgg ccgcgctcga tgccgagcgc
    13021 tggctgga
//
LOCUS       DAOQIE010000067        13020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2337_length_13020_cov_7.820131, whole genome shotgun sequence.
ACCESSION   DAOQIE010000067 DAOQIE010000000
VERSION     DAOQIE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_13020_cov_7.820131"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="rpoH"
                     /locus_tag="PK144_06030"
     CDS             complement(<1..826)
                     /gene="rpoH"
                     /locus_tag="PK144_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNF65321.1"
                     /translation="MTTTALVPYAQQLPAATGDLDAYIRAVSGVPMLDADEEQDLARK
                     LQQRNDLEAARKLVLAHLRFVVHIARGYHGYGLPLGDLIQEGNVGLMKAVKRFDPEHG
                     VRLVSFAVHWIRAEIHEYILRNWRIVKVATTKAQRKLFFNLRSAKQRLGWFTHEEVET
                     VARELGVTPGDVLEMESRLSGNDLTYDPTPDDDDADGPVAPAAYLSNPADDPLAQLEA
                     EDWEARTGHRLGAALEGLDARARDILRRRWLDEDHKPTLHELAAEYGVSAERIRQIE"
     gene            complement(979..1902)
                     /gene="ftsY"
                     /locus_tag="PK144_06035"
     CDS             complement(979..1902)
                     /gene="ftsY"
                     /locus_tag="PK144_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNF65322.1"
                     /translation="MSEASWFGRLKSRLARTRQTLSDGLGSLLLGRKQIDDELFEELE
                     TRLLMADVGIEATQHIIGRLTERVSRRQLADGAALMQALREELTALLAPCERPLDVST
                     QRPFVILMAGINGAGKTTTIGKLAQRFRSEGRSVLLAAGDTFRAAAVEQLQVWGERNG
                     VPVIAQGTGADSASVIFDAVQSAQARGTQVLIADTAGRLHTQQNLMDELRKIRRVIGK
                     IDAAAPHEVLLVLDAGTGQNALNQAVQFHEAIGVTGLVLTKLDGTAKGGIVFAIARRL
                     GLPIRYIGVGEGLEDLREFVAADYVSALLDA"
     gene            2016..2603
                     /gene="rsmD"
                     /locus_tag="PK144_06040"
     CDS             2016..2603
                     /gene="rsmD"
                     /locus_tag="PK144_06040"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNF65323.1"
                     /translation="MPRSPRPSNQLRVIGGRFRGRRLAFPDAPGLRPTPDRVRETLFN
                     WLMPVIDGARCLDLFAGSGALGIEALSRGARLVQFVEREPAVARSLAANLALLGAGDT
                     AALATRDALAWLDGPAQPFDVVFLDPPFHHGLLPAVCERLAAGWLAPQARVYLESESE
                     SGSGQGGADALPPGWTLLRERTAGQVRYRLVTPSA"
     gene            2691..3206
                     /gene="coaD"
                     /locus_tag="PK144_06045"
     CDS             2691..3206
                     /gene="coaD"
                     /locus_tag="PK144_06045"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018867926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNF65324.1"
                     /translation="MSVVALYPGTFDPLTNGHQDLVERGARMFDRLIVAVAANPGKGP
                     LFTLEQRVDHARQILGHLHNVEICGFDILLVNFAKHVGANVILRGLRAVSDFEYEFQL
                     ASMNRRLHAAIETVFLTPAEQYSFISSTLVREVAKFGGDISAFVSPVVERALAEKFGL
                     PPKAPLPGSVK"
     gene            3235..3489
                     /locus_tag="PK144_06050"
     CDS             3235..3489
                     /locus_tag="PK144_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNF65325.1"
                     /translation="MSLMITDECINCDVCEPECPNGAISQGDEIYVIDPNLCTECVGH
                     FDTPQCRGVCPVDCIPNDPSHVETPAQLQEKYQRLSGLIG"
     gene            3589..4095
                     /locus_tag="PK144_06055"
     CDS             3589..4095
                     /locus_tag="PK144_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNF65326.1"
                     /translation="MIERCIETLIFGSRWLLVPLYLGLAAVLVLFSARAVIEIVHVFQ
                     AFPDIGEIDLLLGVLGLIDFVLVANLLVMVILSSYEAFVSRLDVADAQERPGWLGKVD
                     AGTVKIKLAAAIVAVSSIHLLKVFMKIDRYDDRQLWWLCGIHLVFVVSALLMAVVDKL
                     AFAAHREH"
     gene            4236..5090
                     /locus_tag="PK144_06060"
     CDS             4236..5090
                     /locus_tag="PK144_06060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65327.1"
                     /translation="MTIDSAAGSPVPPCVLVLGRDLAAARAYAARLVHAGYRAVAGVP
                     ALDSAPVAALLLGGEGESEGTAWAEAVAPLLGDLPSRIETGPLPEDFAGLAHWLDGLG
                     APVQERRVMSTGLVLSEVADGEAAAGGLLAAADFERRLGVELARCQRYGRVLSFGLLD
                     PDGLSALRSRSPAAADRVLRQLARFTVAHLRRTDVSGRCGAGGIGVLLTETTAARGLG
                     VFERLRVGFVQGQLRVQPRVPAVSLSIGVVECRGERDVAGVLAVAQTAVEAARAAGED
                     CVRLAPAA"
     gene            complement(5103..6167)
                     /locus_tag="PK144_06065"
     CDS             complement(5103..6167)
                     /locus_tag="PK144_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNF65328.1"
                     /translation="MNRLPTLARLGALLLALLSSLACAPARAADERTLVLYNWAEYMP
                     RPVLKQFTKETGIKVRYSTYDNNEAMFAKLKAVEASGGYDLVVPSSYYVTRMRAAGML
                     RPLDKDRLPNLKNLDPRLLDKPYDPGNAYSVPYLWGASGIAVDSARIDPATVTSWADL
                     WKPEYRGRLMLLDDLREVFGIALKLDGRSINSRDPDEIRAAYEKLLELRPNVKVFNAE
                     SPKSVLLSGEVRIGQIYNGEAWMAEQANPKIRFVIPKEGPLLWVDNLVIPKSARHVEE
                     AHAFIDFVLRPEIAKQIAEEVGYTTPNLAARALLAKKVRDSRTAYPSDADLERGEFQT
                     DVGDALPLYEQYWSKLKAAR"
     gene            complement(6390..7442)
                     /locus_tag="PK144_06070"
     CDS             complement(6390..7442)
                     /locus_tag="PK144_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNF65329.1"
                     /translation="MKKLLLVTALLAALPLAAQAEEEKVLNLYNWTEYMPKEVLKAFT
                     KETGIKVKESNYAATEEMYSKLKAGGGGYDLVVPSTYFVQRMAREGLLEPLDHAKLPN
                     LKNVDPKFLDKPFDPGNKYSVPYLMSHVGIVVDGGKVDVSSLKSWLDLWKPEFKGKVL
                     LMDTARETFAVVQKALGYSANDTDPKHIEAAFNKLKELVPSVKLFNGESPKVSFIQGE
                     VSIGIAWNGEAFMGSQEKKSLTYVYPQEGVVLAMDTMAIPKGAKHPENAHKFLDFIMR
                     PEIAAKISETLGYATLNAEAVKLLPKKLASNRIVYPTDEDLKNSELLEDIPTETQGLY
                     EKYWSELKVGKKVKGG"
     gene            complement(7439..8206)
                     /gene="potC"
                     /locus_tag="PK144_06075"
     CDS             complement(7439..8206)
                     /gene="potC"
                     /locus_tag="PK144_06075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HNF65330.1"
                     /translation="MKGLGRGAYLALVYAFLYLPIAVLIVYSFNDARYSLSWQGFTWG
                     WYESLSQNDDLIDVALNSVIVALLSATLATIIGTLGAVALYRYRFSGRNVLNGLIFVL
                     IMSPEIVMGISLLLLFVTMGLELGFTTVLLSHTTFSIPFVVVTVMSRLSGFDKFLIEA
                     AQDLGATEFQTFRKVILPLLIPAVVAGWLLSFTLSMDDVIISFFVTGPDFEVLPLKVY
                     SMVRLGVKPEINALSTVMFALTLVIVLIAQSLLKERK"
     gene            complement(8209..9063)
                     /gene="potB"
                     /locus_tag="PK144_06080"
     CDS             complement(8209..9063)
                     /gene="potB"
                     /locus_tag="PK144_06080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HNF65331.1"
                     /translation="MSDHALFKRVAIGTIAVWLLLFAFAPNLMVVIASFLTRGESEFL
                     VWTPTTDAYARMLDPLYLHVFWNSFYLSAMTTLLCLVIGYPFAYLIARANPAVRPYLL
                     LLVIIPFWTSSLVRTYAMIVILNTNGLLNTLLQSLGLIDEPLELLYTDGAVIAGLVYT
                     LLPFMVLPLYAAIEKLDWRLVEAARDLGARKVRTFASVIIPLTSPGIVAGCMLVFLPG
                     LGMFYVADFLGGSKTTLIGNLIQRQFLSARDWPFGSAASVTLTIVMGLMIGLYLLAAK
                     RANAKVEV"
     gene            complement(9056..10168)
                     /gene="potA"
                     /locus_tag="PK144_06085"
     CDS             complement(9056..10168)
                     /gene="potA"
                     /locus_tag="PK144_06085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007639557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HNF65332.1"
                     /translation="MSEHVIELRSATKAYGKQTVLHGVDLKVRRGEFLTLLGPSGCGK
                     TTILRLLAGFESPTTGTVLLDGQDVTALPPEDRGVNTVFQSYALFPHMTVYDNVAFGP
                     RIKGVGGSELETRVMEALKTVKLDGFAQRKPAQLSGGQQQRVAIARALVNRPLVLLLD
                     ESLSALDYKLRKAMQIELKQLQRQLGITFVFVTHDQEEALSMSDRVVVMNAGNIQQIG
                     TPREVYEQPENLFVAKFVGEINTLTGTVCAVHGDGSYDIDLFGHVIDLRARRSFAVGA
                     EVSVLIRPEDLRIWREGEGTVEDLADLFPGTVEEVIYKGTTVDLIVRLTETRELLSTT
                     QFFNEDDASGSLLDYRIGEPVHVDWVHGWEVILPHE"
     gene            complement(10307..11122)
                     /gene="mutM"
                     /locus_tag="PK144_06090"
     CDS             complement(10307..11122)
                     /gene="mutM"
                     /locus_tag="PK144_06090"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNF65333.1"
                     /translation="MPELPEVETTRRGIAPHLLGRTIARVIVREPRLRWPVPPEFGAN
                     LEAQVIERVERRGKYLLLGNALGFALLHLGMSGSLRILPASTPPGRHEHLDLLLDDGL
                     CLRLTDPRRFGAALWAGPEPHAHPLLAHLGPEPLGPGFTAGGLAARAAGRSQAVKSFI
                     MDSHVVVGVGNIYASEALFLAGIDPRRPAGSVMPPAWLRLVAAIRQVLEAAIAEGGTT
                     LRDFVGVEGKPGYFRQQLRVYDRAGQPCRSCGAPIVQIRQTGRSTYFCACCQS"
     gene            complement(11208..11363)
                     /gene="rpmG"
                     /locus_tag="PK144_06095"
     CDS             complement(11208..11363)
                     /gene="rpmG"
                     /locus_tag="PK144_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNF65334.1"
                     /translation="MRDKIKLVSTAGTGYFYTTTKNKRTMPGKLELKKFDPVVRQHVL
                     FKEAKIK"
     gene            complement(11386..11622)
                     /gene="rpmB"
                     /locus_tag="PK144_06100"
     CDS             complement(11386..11622)
                     /gene="rpmB"
                     /locus_tag="PK144_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNF65335.1"
                     /translation="MSRVCQVTGKGPITGNNVSHANNKTRRRFLPNLHEHRFWVESEN
                     RFVRLRVSSAGMRVIDKKGIDAVLAELRGRGVNV"
     gene            complement(11792..12466)
                     /gene="radC"
                     /locus_tag="PK144_06105"
     CDS             complement(11792..12466)
                     /gene="radC"
                     /locus_tag="PK144_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNF65336.1"
                     /translation="MSIRDWPADERPREKLLARGAQALSDAELLAIFLRTGMPGKSAV
                     DLARELLVQFGSLRALLEADLRSFCSGPGLGEAKYVQLQAVLELASRHLESTLTRGDA
                     LASVTHTRRYLTARLRHQPHEVFAVLFLDTRHRVIAYEELFHGTIDGTSVHPRQVVKR
                     ALHHNAAALILAHNHPSGVAEPSRADESITLRLKEALALIDVRVIDHFVIGDGQSVSF
                     AERGLL"
     gene            12668..>13020
                     /locus_tag="PK144_06110"
     CDS             12668..>13020
                     /locus_tag="PK144_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNF65337.1"
                     /translation="MDLKGSQTENNLKAAFAGESQANRRYLYFAAKADVEGYNDVSAV
                     FRSTAEGETGHAHGHLEYLEASGDPATGLPIGPTASNLKAAIAGETHEYTDMYPGMAR
                     TAREEGFEEIADWFET"
BASE COUNT         2000 a         4273 c         4590 g         2157 t
ORIGIN      
        1 cctcgatctg gcggatgcgc tcggccgaga cgccgtactc ggcggccagc tcgtgcagcg
       61 tcggcttgtg gtcctcgtcg agccagcgcc ggcgcaggat gtcgcgggcg cgggcatcga
      121 ggccttcgag cgcggccccg agccggtggc cggtgcgcgc ctcccagtcc tcggcttcga
      181 gctgcgcgag cggatcgtcg gccggattcg acaggtacgc ggccggcgcc accgggccgt
      241 cggcgtcgtc gtcgtccggc gtcgggtcgt aggtcaggtc gttgccggac agccggcttt
      301 ccatctccag cacgtcgccg ggggtcacgc cgagttcgcg cgcgacggtc tcgacttcct
      361 cgtgcgtgaa ccagccgagg cgctgcttgg cgctgcgcag gttgaagaac agcttgcgct
      421 gcgccttcgt ggtcgcgacc ttgacgatgc gccagttgcg caggatgtat tcgtggatct
      481 cggcgcggat ccagtgcacg gcgaagctga cgaggcgcac gccgtgctcc gggtcgaagc
      541 gcttgacggc cttcatcagg ccgacgttgc cttcctggat caggtcgccg agcggcagcc
      601 cgtagccgtg gtagccgcgg gcgatgtgca cgacgaagcg caggtgcgcg agcacgagct
      661 tgcgcgccgc ttcgaggtcg ttgcgttgct gcagcttgcg ggcaaggtcc tgttcctcgt
      721 ccgcgtcgag catcggcacc ccggaaaccg cgcggatgta ggcgtcgagg tcgccggtgg
      781 cggccggaag ctgttgcgcg tagggaacca gtgcggtcgt ggtcatgcga agtcctccag
      841 agaactccag ttcgcgggct gatgttagca gtcgggcgat aggagtgcca aagccggcgg
      901 gagttccgcc ggcgcccccg gttgagcggc gatacgcgcc tgtatcggca gcggtttcgc
      961 gaagcttctc gcgaagcttc aggcgtcgag cagcgccgag acgtaatcgg cggcgacgaa
     1021 ctcgcgcagg tcctccagcc cctcgccgac gccgatgtag cggatcggca ggccgaggcg
     1081 gcgcgcgatc gcgaacacga tgccgccctt ggcggtgccg tcgagcttgg tcagcaccag
     1141 accggtgacg ccgatcgcct cgtggaactg cacggcctgg ttcagcgcgt tctggccggt
     1201 gccggcgtcg agcaccagca gcacctcgtg cggcgcggcg gcgtcgatct tgccgatgac
     1261 gcggcggatc ttgcgcagct cgtccatcag gttctgctgc gtgtgcaggc gcccggcggt
     1321 gtcggcgatc agcacctgcg tgccgcgcgc ctgcgccgac tgcacggcgt cgaagatcac
     1381 cgaggccgaa tcggcaccgg tgccctgcgc gatcaccggc acgccgttgc gctcgcccca
     1441 gacctgcagc tgctcgacgg cggcggcgcg gaaggtgtcg ccggcggcga gcagcacgct
     1501 cctgccctcg ctgcggaagc gctgcgcgag cttgccgatg gtcgtggtct tgccggcgcc
     1561 gttgatgccg gccatcagga tcacgaacgg ccgctgcgtg ctcacgtcga gcgggcgctc
     1621 gcagggcgcg agcagcgcgg tcagctcctc gcgcagcgcc tgcatcagcg ccgcgccgtc
     1681 ggcgagctgg cggcgcgaca cgcgctcggt cagccgcccg atgatgtgct gcgtggcctc
     1741 gatgccgaca tcggccatca gcagccgggt ttcgagttcc tcgaacagct cgtcgtcgat
     1801 ctgcttgcgc ccgagcagca ggctgccgag gccgtcgctg agggtctggc gggtgcgggc
     1861 gagtcggctc ttcaggcggc cgaaccagga ggcttcgctc attgcgcggg ggattctcgt
     1921 gcagggacat ggcccgcgcg cggcgggcgg ggcgcgcgca tcgtatcatc gccgcccccg
     1981 gccgcctgcc gttgccgatc gcccgagccc tcccgatgcc ccgcagtccc cggccgagca
     2041 accagttgcg cgtgatcggc ggccgcttcc gcggccggcg gctcgcgttc cccgacgctc
     2101 cgggcctgcg cccgacgccg gatcgcgtgc gcgagactct gttcaactgg ctgatgccgg
     2161 tcatcgacgg cgcgcgctgc ctcgacctgt tcgccggcag cggtgcgctc ggcatcgagg
     2221 cgctgtcgcg cggggcgcgg ctcgtgcagt tcgtcgagcg cgagccggcg gtcgcgcgct
     2281 cgctcgcggc caacctcgcg ctgctcggtg ccggcgacac cgcggccctc gcgacgcgcg
     2341 acgcgctggc ctggctcgac ggaccggcgc agcccttcga cgtggtgttc ctcgatccgc
     2401 cgtttcacca cggcctgctg ccggcggtct gcgagcggct cgccgccggc tggctggcgc
     2461 cgcaggcgcg ggtctatctc gaaagcgaaa gcgaaagcgg gagcgggcag ggcggcgccg
     2521 atgcgctgcc cccgggctgg acgctgctgc gcgagcgcac ggccgggcag gtccgctatc
     2581 ggctcgtgac gccatctgct tgaccggggt caggtttatg accttgtgag gccggggacg
     2641 gcaacgcata atccgtcgcc cttttccgac cagtgccgac aggtatccgc atgagcgtcg
     2701 ttgccctgta cccgggtacg ttcgatccgc tgaccaatgg tcatcaggat ctcgtcgagc
     2761 gcggcgcccg catgttcgac cggctgatcg tcgcggtggc ggccaatccc ggcaagggtc
     2821 cgctgttcac gctcgaacag cgcgtcgatc acgcccgcca gattctcggt cacctgcaca
     2881 acgtcgagat ctgcggcttc gacatcctgc tggtcaattt cgccaagcac gtcggcgcca
     2941 acgtcatcct gcgcggcctg cgcgcggtgt cggatttcga atacgaattc cagctcgcca
     3001 gcatgaaccg ccgcctgcac gcggcgatcg agaccgtgtt cctgacgccg gccgagcagt
     3061 actcgttcat ttcctcgacg ctggtgcgcg aggtcgcgaa gttcggcggc gacatctccg
     3121 ccttcgtcag tccggtggtc gagcgtgcgc tggccgagaa gttcggactg ccgccgaagg
     3181 caccgctgcc cggttccgtg aagtaacggc ccgtccgggc cggaggtcca tgccatgtcc
     3241 ctgatgatta ccgacgaatg catcaactgc gatgtctgcg agccggagtg cccgaacggc
     3301 gcaatctcgc agggtgacga gatctacgtc atcgacccga acctgtgtac cgaatgcgtc
     3361 ggccatttcg atacgccgca gtgtcgcggt gtctgtccgg tcgactgcat cccgaatgac
     3421 ccgagccacg tcgaaacgcc cgcgcagctg caggaaaagt atcagcgcct gtccggcctg
     3481 atcggctgac gagccgcggc cggaaccatc gggccccgct cgccggggcc ttttttattt
     3541 ccctgaccgg acacctgcca ggcagggtcc ggcgcaagcc cgggcatcat gatcgaacgc
     3601 tgcattgaaa ccctgatctt cggcagccgc tggctgctcg ttccgctgta cctcggtctg
     3661 gccgcggtgc tggtgctgtt ctcggcccgg gccgtgatcg agatcgtgca tgtcttccag
     3721 gcatttcccg acatcggcga gatcgacctg ctgctcggcg tgctcggact gatcgacttc
     3781 gtgctggtcg ccaacctgct ggtcatggtg atcctgtcga gctacgaggc cttcgtctcg
     3841 cgtctcgatg tcgccgacgc tcaggagcgc ccgggctggc tcggcaaggt cgatgccggc
     3901 accgtcaaga tcaagctcgc ggccgcgatc gtcgcggtgt cgtcgatcca cctgctgaag
     3961 gtgttcatga agatcgaccg ctacgacgac cgccagctct ggtggctgtg cggcatccac
     4021 ctggtgttcg tggtctcggc gctgctgatg gccgtggtcg acaagctggc cttcgccgcg
     4081 caccgcgagc actgatccgg cttcattcgg gcacgccggt tcaaggccgc cggcgggcag
     4141 ccgacagtat tgcttcggac ccgggcacca tggctcgtgg catgcgcaat actcgtcggt
     4201 gatccagaga ttgccgaaga gccggaggcc aggtgatgac catcgattca gcagcgggaa
     4261 gcccggtgcc gccctgcgta ctggtgctgg ggcgcgatct cgccgccgcc cgcgcctacg
     4321 ccgcgcggct ggtgcatgcc ggttaccggg ccgtcgccgg ggtgccggcg ctcgattcgg
     4381 cgccggtcgc ggcgctgctg ctcggcggcg aaggcgaaag cgaaggcacg gcctgggccg
     4441 aagcggtggc gccgctgctc ggtgacctgc cgagccgcat cgagaccggt ccgctgcccg
     4501 aggatttcgc cggcctcgcg cactggctcg acgggctcgg cgcgccggtg caggaacgcc
     4561 gggtgatgag cacggggctg gtgctcagcg aggtcgccga cggcgaggcc gcggccggcg
     4621 gcctgctggc ggccgccgac ttcgagcggc gcctcggcgt cgaactggcg cgctgccagc
     4681 gctacgggcg ggtgctgagc ttcgggctgc tcgatccgga cggcctgtcg gcgctgcgca
     4741 gccgcagccc cgccgccgcc gaccgcgtcc tgcgccagct tgcgcgcttc acggtcgcgc
     4801 acctgcgccg caccgatgtc agcggccgct gcggcgccgg cggcatcggc gtgctgctga
     4861 ccgaaaccac ggctgcgcgc ggcctcggcg tgttcgaacg gctgcgcgtc ggtttcgtgc
     4921 aggggcagct gcgcgtgcag ccgcgcgtgc cggccgtgag cctcagcatc ggcgtcgtcg
     4981 aatgccgcgg cgagcgcgat gtcgccggcg tgctggcggt cgcgcagacg gcggtcgagg
     5041 ccgcgcgggc cgccggcgag gactgcgtgc gcctggcccc ggcggcctga ggcggcgcgg
     5101 cctcagcgcg cggccttcag cttcgaccag tactgctcgt acagcggcag cgcgtcgccg
     5161 acatcggtct ggaactcgcc gcgttcgaga tcggcatcgg acggataggc cgtgcggctg
     5221 tcgcggacct tcttcgcgag cagcgcgcgc gcggcgaggt tcggcgtcgt gtagccgacc
     5281 tcctcggcga tctgcttcgc gatctcgggg cgcagcacga agtcgatgaa ggcgtgggct
     5341 tcctcgacgt ggcgcgcgct cttcgggatc acgaggttgt cgacccacag cagcgggcct
     5401 tccttcggga tcacgaagcg gatcttcgga ttggcctgct cggccatcca ggcctcgccg
     5461 ttgtagatct ggccgatgcg cacctcgccg gacagcagca ccgacttcgg cgactcggcg
     5521 ttgaacacct tcacgttcgg acgcagttcg agcagctttt cataggcggc gcggatttcg
     5581 tcgggatcgc ggctgttgat cgaacggccg tcgagcttca gcgcgatgcc gaacacctcg
     5641 cgcaggtcat cgagcagcat cagccggccg cggtactcgg gtttccacag atcggcccag
     5701 ctcgtgaccg tcgccgggtc gatgcgcgcg ctgtcgacgg cgatgccgct cgcgccccac
     5761 agatacggga cgctgtaggc gttgccgggg tcgtagggct tgtcgagcag gcgcgggtcg
     5821 aggttcttca ggttcggcag ccggtccttg tcgagcggcc gcagcatgcc ggcggcgcgc
     5881 atgcgcgtga cgtaatagct cgacggcacg accaggtcgt agccgccgga ggcctcgacg
     5941 gccttgagct tcgcgaacat cgcctcgttg ttgtcgtagg tcgaatatct gaccttgatg
     6001 ccggtttcct tcgtgaactg cttcagcacc gggcgcggca tgtactcggc ccagttgtac
     6061 agcaccagcg tgcgctcgtc ggcggcgcgg gccggggcgc aggcgagact cgacagcagg
     6121 gcgagcagca gcgccccgag gcgcgctagg gtcggcagac ggttcatcgg cgggcctctt
     6181 gggcagcagg gggacacgca tgcgccggtc gcgggcggga gccgcaacct tgggcactgc
     6241 cggcgaggcc cgcagacccg aaagccgcac gggccgcaga ggcgtgaccg caggcgcacg
     6301 cgccgtcagt gttgccgctg ccggcgggct tgcaagaaaa aaccccgccg gtgcggcggg
     6361 gttttgcggg cgcctcggaa gcggacggct cagccgccct tgaccttctt gccgaccttc
     6421 agctccgacc agtacttctc gtacaggccc tgcgtctcgg tcgggatgtc ttcgagcagc
     6481 tcggagttct tcaggtcctc gtcggtcggg tagacgatgc ggttcgaggc gagcttcttc
     6541 ggcagcagct tcacggcctc ggcgttgagc gtcgcatagc cgagcgtttc ggaaatcttc
     6601 gccgcgattt ccggccgcat gatgaagtcg aggaacttgt gtgcgttttc ggggtgcttc
     6661 gcacccttcg gaatcgccat cgtatccatc gccagcacga cgccttcctg cggatagacg
     6721 taggtcagcg acttcttttc ctgcgagccc atgaaggctt cgccgttcca ggcgatgccg
     6781 atcgaaacct cgccctggat gaagctgacc ttcggcgatt cgccgttgaa cagcttcacg
     6841 ctcggcacga gttccttgag cttgttgaac gcggcctcga tgtgcttcgg atcggtatcg
     6901 ttcgcggaat agccgagcgc cttctgcacg accgcgaagg tttcgcgggc cgtatccatc
     6961 agcaggacct tgcccttgaa ttccggcttc cagagatcga gccagctctt gaggctgctc
     7021 acatcgacct tgccgccgtc gacgacgatg ccgacgtgcg acatcaggta ggggacgctg
     7081 tacttgttgc ccggatcgaa gggcttgtcg agaaacttcg gatcgacgtt cttgaggttc
     7141 ggcagcttgg cgtggtcgag cggctccagc aggccttcgc gcgccatgcg ctgcacgaag
     7201 tacgtggacg gcacgaccag gtcatagccg ccgccgccgg ccttgagctt cgaatacatc
     7261 tcctcggtcg cggcgtaatt ggattccttg accttgatgc cggtttcctt cgtgaaggcc
     7321 ttcaggacct ccttcggcat gtactcggtc cagttgtaaa ggttcaggac cttttcctct
     7381 tcggcctgcg ccgcgagcgg aagcgcggcg agcagtgcgg tgaccagcag cagctttttc
     7441 atttcctctc cttcaaaagg gactgggcga tcagcacgat cacgagcgtc agtgcgaaca
     7501 tcacggtgga gagcgcgttg atttccggct tcaccccgag gcgcaccatc gagtacacct
     7561 tcagcggcag gacctcgaaa tccggtccgg tcacgaagaa gctgatgatc acgtcgtcca
     7621 tcgacagcgt gaacgacagc agccagccgg ccacgacggc cgggatcagc agcggcagga
     7681 tcaccttgcg gaaggtctgg aactcggtcg ccccgagatc ctgcgccgcc tcgatcagga
     7741 acttgtcgaa accgctcagg cggctcatga ccgtgaccac gacgaagggg atcgagaagg
     7801 tcgtgtgcga cagcagcacg gtcgtgaagc cgagttccag ccccatggtc acgaacagca
     7861 gcagcagcga gatgcccatg acgatctccg gcgacatgat cagcacgaag atcaggccgt
     7921 tgagcacatt gcggccgctg aagcggtagc ggtacagcgc gacggcaccg agcgtgccga
     7981 tgatggtcgc gagcgtggcc gacagcagcg cgacgatcac cgagttcagc gccacgtcga
     8041 tcaggtcgtc gttctggctc agggactcgt accagcccca ggtgaagccc tgccacgaga
     8101 gcgagtacct ggcatcgttg aacgagtaga cgatcagcac cgcgatcggc aggtacagga
     8161 aggcgtagac gagcgcgaga tacgctccgc ggccgaggcc cttcatgctc agacctccac
     8221 cttcgcgttc gcgcgcttcg cggcgagcag atacagcccg atcatcaggc ccatgacgat
     8281 ggtcagcgtc acgctcgccg ccgagccgaa cggccagtcg cgcgccgaca ggaactggcg
     8341 ctggatcagg ttgccgatca gcgtggtctt ggagccgccg aggaagtccg cgacgtagaa
     8401 catgccgagg ccgggcagga acaccagcat gcagccggcg acgatgccgg gcgaggtcag
     8461 cgggatgatc accgaggcga aggtccggac cttgcgcgcg ccgaggtcgc gcgccgcctc
     8521 gaccagccgc cagtcgagct tctcgatcgc cgcatacagc ggcagcacca tgaacggcag
     8581 cagcgtgtag acgaggccgg cgatcacggc gccgtcggtg tacagcagtt cgagcggctc
     8641 atcgatcagg ccgagcgact gcagcagcgt gttcagcagg ccgttggtgt tcaggatcac
     8701 gatcatcgcg taggtgcgca ccagcgagct ggtccagaac gggatgatca cgagcagcag
     8761 caggtagggg cgtacggcgg gattcgcgcg cgcgatcagg tacgcgaacg gatagccgat
     8821 cacgaggcag agcagcgtcg tcatcgccga caggtagaac gaattccaga agacgtgcag
     8881 gtacagcggg tcgagcatgc gcgcgtaggc gtcggtggtc ggcgtccaga ccaggaactc
     8941 gctctcgccg cgggtcagga agctcgcgat gacgaccatc aggttcggtg cgaacgcgaa
     9001 cagcagcagc cagaccgcga tcgtgccgat cgcgacacgc ttgaacaggg cgtggtcact
     9061 catgggggag gatgacctcc cagccgtgga cccagtcgac gtgcaccggc tcgccgatgc
     9121 ggtagtcgag gaggctgccc gaggcgtcgt cctcgttgaa gaactgcgtc gtcgacagca
     9181 gctcgcgggt ctcggtcagg cgcacgatga gatcgacggt cgtgcccttg tagatgactt
     9241 cctcgaccgt gccggggaag agatcggcga gatcctcgac cgtgccttcg ccttcgcgcc
     9301 agatgcgcag gtcctcgggc cggatcagca cgctgacctc ggcgccgacg gcgaagctgc
     9361 gccgcgcgcg caggtcgatg acgtgaccga acaggtcgat gtcgtagctg ccgtcgccgt
     9421 gcacggcgca gaccgtgccg gtcagcgtgt tgatctcgcc gacgaacttg gcgacgaaca
     9481 ggttctcggg ctgttcgtag acctcgcgcg gcgtgccgat ctgctggatg ttgccggcgt
     9541 tcatgaccac gacgcggtcg gacatcgaca gcgcctcttc ctggtcatgg gtcacgaaca
     9601 cgaaggtgat gccgagctgg cgctgcagct gcttcagctc gatctgcatg gccttgcgca
     9661 gcttgtagtc gagcgccgac agcgattcat cgagcagcag caccagcggc cggttgacca
     9721 gcgcgcgggc gatggcgacg cgctgctgct ggccgccgga gagctgcgcg ggcttgcgct
     9781 gcgcgaagcc gtcgagcttg acggtcttca gcgcctccat gacgcgggtt tcgagttcgc
     9841 tgccgccgac gcccttgatg cgcggaccga aggcgacgtt gtcgtacacc gtcatgtgcg
     9901 ggaacagcgc gtagctctgg aacaccgtgt tgacgccgcg atcctcgggc ggcagcgcgg
     9961 tcacgtcctg gccgtcgagc agcacggtgc cggtggtcgg ggattcgaag ccggcgagca
    10021 ggcgcaggat ggtggtcttg ccgcaaccgg aagggccgag cagggtcagg aactcgccgc
    10081 ggcggacctt caggtcgacg ccgtgcagga ccgtctgctt cccgtaagcc ttggtggcac
    10141 tgcgcaattc gatgacgtgt tcactcatgg tccggacggc gaaggcggga gggaatcagg
    10201 ggtgaaacag ccgggcaggg cgaccggaag cgcggctaaa catacacgaa ttcccgaagc
    10261 cgccgacggc gttttttgca ggtgcagcgc agcccggcgg ggggcttcag gactggcagc
    10321 acgcgcagaa gtaggtcgag cgaccggtct ggcggatctg cacgatcggt gcgccgcagc
    10381 tgcggcaggg ctggccggcg cggtcgtaga cgcgcagctg ctggcggaag tagccgggtt
    10441 tgccctcgac gccgacgaag tcgcgcagcg tcgtgccgcc ctcggcgatc gcggcttcga
    10501 gcacctgacg gatcgccgcc accagccgca gccaggccgg cggcatgacg ctgccggccg
    10561 gccggcgcgg atcgatgccg gcgagaaaca gcgcctcgga tgcgtagatg ttgccgacgc
    10621 cgacgacgac gtggctgtcc atgatgaagc tcttgaccgc ctggctgcgg ccggcggcgc
    10681 gcgcagccag gcccccggcc gtgaagccgg ggccgagcgg ctcggggccg agatgcgcga
    10741 gcagcggatg cgcgtgcggc tcggggccgg cccacagcgc ggcgccgaag cggcgcgggt
    10801 cggtgaggcg caggcagagg ccgtcgtcga gcagcaggtc gagatgctcg tggcggccgg
    10861 gcggcgtgct cgcgggcagg atgcgcaggc tgccggacat gccaaggtgc agcagcgcga
    10921 agccgagcgc gttgccgagc agcaggtact tgccgcggcg ctcgacgcgt tcgatcacct
    10981 gcgcttcgag gttggcgccg aactccggcg gcaccggcca gcgcaggcgc ggctcgcgca
    11041 cgatcacgcg cgcgatcgtg cgtccgagca ggtgcggcgc gatgccgcgg cgggtggttt
    11101 cgacttcggg cagttcgggc atcggggccg agggagcagc gcggaaggcg gcggacaaga
    11161 aaaaacccgc cgcagcgggt cttttcgtca gcgggccgcg gcgcggctca cttgatcttg
    11221 gcttccttga acagcacgtg ctggcggacg accggatcga acttcttcag ttcgagcttg
    11281 ccgggcatcg tgcgcttgtt cttggtggtg gtgtagaagt agccggtgcc ggccgtcgag
    11341 acgagcttga tcttgtcgcg catgacgaat tccccggggg ttggctcaga cgttcacgcc
    11401 gcggccgcgc agctcggcca gcacggcatc gatgcctttc ttgtcgatga cacgcatgcc
    11461 ggcgctcgac acgcgcaggc gcacgaagcg gttctcgctc tcgacccaga agcggtgctc
    11521 gtgcaggttc ggcaggaagc ggcgacgcgt cttgttgttc gcgtgcgaga cgttgttacc
    11581 ggtgatcgga cccttgccgg tcacctgaca gactctggac atggaaaaag cctccgtaaa
    11641 atgcgcgccc gccgcgtcag ggcaggcgta tgccgccacc gttcggcacc ggcggcggac
    11701 gagtgtgcgg attgcggcga ccgccggata cccgggcgga ccgcgaaagg cgggctttat
    11761 agcagagccc gccggcgggc ggcaaggcgg atcacagcag tccgcgctcg gcgaaggaca
    11821 ccgactgacc gtcgccgatg acgaagtgat cgatcacgcg cacgtcgatc agcgccagcg
    11881 cctccttcag gcgcagcgtg atcgattcgt cggcgcggct cggctcggcg acgccggacg
    11941 gatggttgtg cgcgaggatc agcgccgcgg cgttgtggtg cagcgcgcgc ttcaccacct
    12001 ggcgcggatg caccgaggtg ccgtcgatgg tgccgtggaa gagttcctcg taggcgatca
    12061 cgcggtggcg cgtgtcgagg aacagcaccg cgaacacctc gtgcggctga tggcgaagcc
    12121 tcgccgtcag gtagcggcgc gtgtgcgtga cgcttgcgag cgcatcgccg cgcgtcagcg
    12181 tcgattcgag gtggcggctc gcgagttcga gcacggcctg caactgcacg tacttggcct
    12241 cgccgagtcc cgggccgctg cagaagctcc gcaggtcggc ttcgagcagc gcgcgcaggc
    12301 tgccgaactg cacgagcagc tcgcgcgcca gatcgaccgc gcttttgccg ggcatgccgg
    12361 tgcgcaggaa gatcgcgagc agctcggcat cggacagcgc ctgcgcgccg cgtgcgagca
    12421 gtttctcgcg cgggcgctcg tcggccggcc agtcacggat cgacatcggg ttcacctccc
    12481 tgtgctcgac aaaacgcggg ggcatagcgc gtggctatcg cagcgatgcg gattttcgaa
    12541 tggtgaatcc ccggatcgct tctagagtct aggccgcacc cgtcaggggt gcgccggttc
    12601 gccatcgcag cgaccggcgt cttgccttcg ggcagcctgc catcgcaatg agtgagaggt
    12661 gagagtcgtg gatctgaagg gcagtcagac cgagaacaac ctgaaggctg cgtttgccgg
    12721 cgaatcccag gccaaccgcc gctacctgta tttcgcagcc aaggccgatg tcgagggcta
    12781 caacgacgtg tcggcggtgt tccgctcgac cgccgaaggc gagaccggcc acgcgcacgg
    12841 ccacctcgaa tacctcgaag ccagcggcga cccggccacc ggcctgccga tcggcccgac
    12901 cgcctcgaac ctgaaggccg cgatcgccgg cgagacgcac gagtacaccg acatgtaccc
    12961 gggcatggcg cgcaccgccc gcgaggaggg cttcgaggag atcgccgact ggttcgagac
//
LOCUS       DAOQIE010000068        13011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2343_length_13011_cov_7.356823, whole genome shotgun sequence.
ACCESSION   DAOQIE010000068 DAOQIE010000000
VERSION     DAOQIE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2343_length_13011_cov_7.356823"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PK144_06115"
     CDS             complement(<1..754)
                     /locus_tag="PK144_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF65338.1"
                     /translation="MSKITRITVRGYKSIRALEAFELRDLNVLIGANGAGKSNFISLF
                     RLLTAVSERRLQLYVQKQGGPDALLHRGRKHTEHMEYLVELDKYGYFAQLEATADNRL
                     IFSDERIVAGSVNRRLGVGHAETGIKRASSSTGIQLVNKSIIDSRVFHFHDTSDNARI
                     KQIHHTHINLRLLHDAANLAAFLLRLRKDYPDFYLRIVETIRLAAPFFGDFVHREPVG
                     ESTQLEWTEYNDPDTPLKAHMLSDGTLRFICLT"
     gene            complement(787..6432)
                     /gene="uvrA"
                     /locus_tag="PK144_06120"
     CDS             complement(787..6432)
                     /gene="uvrA"
                     /locus_tag="PK144_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF65339.1"
                     /translation="MTDPRTPVVPATGAIRLRDAHQNNLKHLDLDFPTGRLTVVTGVS
                     GSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRQDRPQVGSVAGIPPAIAIDQTNPV
                     RTSRSTVGTMTELNDHLKLLYARMSTPCCRGCGRPVRRDGPDSILDDLHARAAAAGDP
                     RLAVSFPVAVRDQAGADVEALLAQQGYTRIQAREPGWLHVVQDRFRLGTAERARVIEA
                     LETALRQGRGRVDVHVLDPAPAPDGDSPEAPAPRWRYSADLHCPDCDIHYAEPVPAAF
                     SFNSPLGACPICRGFGRVIDVDFGLVVPDESKTLAQGAVKPWQTESFRECQQDLEKYA
                     RLRGVALDVPFRDLPPEHRHWVLEGEDGWQSWERSWPKLWYGVRHFFDWLESKAYKMH
                     IRVLLSKYRAYTPCTACAGARLKPESLDFRLGTRAEADAVLAPARRFLPTQVRYRRAV
                     LETLPGLTLHDLMLLPLTRLASFFAQLRLPAPLDEATGLVLGEIRTRLRFLGEVGLGY
                     LTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRVIAVMQRLR
                     AQGNTLVVVEHDPQIMFAADRLLDLGPGPGARGGEVMFFDAPARLAGARGSLTADYLL
                     GRRHVAAGRVPRPVDDNTPRLRLCGAAEHNLKAIDVAIPLGRLVCVTGVSGSGKSTLV
                     QEVLVPALARRLGRPTEAPGAFRALEGGLEIGEVVVVDQSPIGKTARSNPASYIGAFD
                     AIRKLFAGTDAARERGYTPGTFSFNAGNGRCPGCQGSGFEHVEMQFLSDVYLRCPDCD
                     GTRFRAEVREVELAGKSIADVLDLSVAEAIDFFGPLRGGREVVRLLAPLAEVGLDYLH
                     LGQPVPTLSGGEAQRLKLAGHLVESAGRQRRRRGDEPEPQTLFVFDEPTTGLHFDDIA
                     RLLQAFGRLLDAGHTLLVIEHNLDVIDAADWLLDLGPEGGDDGGELLCAGPPDAVIAC
                     AASHTGRALAQYRADMQRLAEGRADAVAEAPASYAAAPPDAIRIHHAREHNLKNVSVE
                     IAHRGFTVITGPSGSGKSTLAFDIVFGEGQRRYLESLNAYARQFVQPAARPEVDAVHG
                     IPPTVAIEQRVSRGGRKSTVATLTETYHFLRLLWMKLGTQYCPDCARPIEPQSEDAIA
                     AKLMAQHRGSRITLLAPLVVHRKGLYTDLAKWAHGKGYERLRVDGDWLPTRPWPRLDR
                     FREHTLELPVAELAVLPNTERELRAALAEALAIGKGVLHVLAGEALQVWSTKRACPAC
                     GTSFPELDPRLFSFNSKHGWCEGCQGTGVRVAGFQRDDRLSEEQVDRALDDWLDARGD
                     DTPCPDCAGERLNPVARAVRFRGLSIGALTARPVAAVAGFLDELALAGREADIAGGLV
                     AEIGSRLGFLQEVGLGYLTLDRAAPTLSGGESQRIRLAAQLGSSLSGVAYVLDEPTIG
                     LHPRDNRILLDALEKLEARGNTLIVVEHDEDTIRRAHHIIDLGPGAGRLGGEVVAEGT
                     ADELAANPASPTGRCLAAPLQHPLQPRRPVEPGQPAIELLGASRHNLKNVCARVPLGR
                     LVVVTGVSGSGKSTLARDVLYTNLHALVAARGKGRNAQPVAIELLGCALLRGWEPVLR
                     VLEVDQTPIGKTPRSCPATYVGFWDAIRKLYADTREARIRGWSASRFSFNTAGGRCPV
                     CEGSGTVRVEMNFLPDVKLPCEACGGARFDAATLEVEWRGLSIGAVLALSVDEAVEFF
                     AAHPLIHHPLKLMQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELAKVKTSLRTGRTTP
                     HTLYVLDEPTVGLHMADVDKLARVLHRLVDAGNTVVLIEHNLDLIAEADWLLDLGPEG
                     GAAGGELVAQGTPEAVMAQAGASHTGAVLAEFMAGRSQP"
     gene            complement(6441..6857)
                     /locus_tag="PK144_06125"
     CDS             complement(6441..6857)
                     /locus_tag="PK144_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNF65340.1"
                     /translation="MIIADTGFWIALAMPDDRFHERACRQLADLKERLIVTWPVITET
                     CHLLGKRVSRHAQQLFLQHLATGVLDIFDLRDIHLPRVIELMQRYANLPMDLADASLV
                     VLAEHLGDGRILSTDMRDFGTYRWKNTAPFRNLLAD"
     gene            complement(6844..7107)
                     /locus_tag="PK144_06130"
     CDS             complement(6844..7107)
                     /locus_tag="PK144_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNF65341.1"
                     /translation="MRIQSRLDEAHARKLDEIRRMTGASISHVIEQALDLYHDRLVTG
                     QPSTYQALLDSGFIGCGPQGQTELSTEYKKAFAAIVEDGHDHR"
     gene            7428..8048
                     /locus_tag="PK144_06135"
     CDS             7428..8048
                     /locus_tag="PK144_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65342.1"
                     /translation="MSHVLSRIPSFNALRGLCALLLLAASVFAHAFGLGLGGPSDTEP
                     AARAGWWYAAPGVETRFELQAGETRMLPLGELVVGAFDSARVLVPGVPADEDRYRLEF
                     ELQPEGSAPVHISASAVVGRAGDAHGERLTFDFGAEPVQIGFGRGGVLSVHFRPLDLA
                     DNGRYDLYAEVRLVSPPAELPEPDLVWQLGLGLVLLALVRHLRRRS"
     gene            8137..9000
                     /locus_tag="PK144_06140"
     CDS             8137..9000
                     /locus_tag="PK144_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65343.1"
                     /translation="MPAELETLIRQLREDRAAGAIELARQALAGLGAYAERVPARSVP
                     GLRASLAEAAMQVTASRPAVTPLMTLVNDWHGRIERVEAGTLQALRAHAVVSAARLSE
                     HSRSAGASTAANVAAWIGPGRTVLTHGWSTTVLACLAQIAALGGLRVICTEARPLNEG
                     RQLAARAAGLGVPVTLITDAQLALAATAADVVLVGADTVLVDGAVVGKAGTWPMALAA
                     RAVDIPFVVAAESCRRSPELSVSLEDQDPAEVGAPLPGVMLRNRRFDVTPPDLVTAWI
                     DEHGVWTGEAA"
     gene            9093..9731
                     /locus_tag="PK144_06145"
     CDS             9093..9731
                     /locus_tag="PK144_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNF65344.1"
                     /translation="MTGIHDFPAFLLASVALAVLPGPDTLYVLGRTVAQGRRAGLWSL
                     AGIGGGCLGHVAASALGLSALIAASPFAFELVKLAGALYLAWIGVQLLREAWRHAGDG
                     VPLPAVAADEAGGWQLLRQGVVTNLLNPKVGLFFISFLPQFVTPGVGTVGDFVLLGLT
                     FVGIGLVWILLLMHTTDLATRHFRGRAAVQRWFKASTGGLFVGLAAKLALDR"
     gene            9754..10584
                     /locus_tag="PK144_06150"
     CDS             9754..10584
                     /locus_tag="PK144_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNF65345.1"
                     /translation="MERNHARASDGAVLAACRHGGHLLSWQTPDGRERLFLSPLSQPA
                     PGVAIRGGVPVCFPQFSGFGPLPKHGFVRDRIWQCTAAGTAADGRARLTYALEADAAT
                     RALWPQAFRCELELLAAGDTLAVTLAVHNTGEAPLSFTGALHTYFAVTDLDAARVSGL
                     EAHPYRDHAGAAAAGGGIDHSPDGAPLCFGAEVDRVYAGVRGAIRLATGAGTLQIEAD
                     GFPDAVIWNPGPEKGAALADLEAGAWRRYVCVEAAIVRTPVSVAAGALWRGTQRVRVI
                     "
     gene            10581..11909
                     /locus_tag="PK144_06155"
     CDS             10581..11909
                     /locus_tag="PK144_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF65346.1"
                     /translation="MTDAATAVPSRAAILRFAAPVMLANLTTPLLGIVDTAIMGRSPD
                     PALIAAVAVGSTVLTVLFWAFSFLRQSTVGLTAQALGAGDATELAAALLRPLALAALI
                     GTVLILLQAPIAALAFGLMTSAPEVETAARAYYGWRIAAAPFALANYALAAWLIGQRR
                     ARLVLGLQLAMNGGNAALTALFVLGFGLGPAGAGLGTALAEMLTCLATLGLPAVRAQF
                     APAVLRAVCSGGRAQFARLMRANRDILLRTLVLTLTLASFTRFGGTLSTATLAANQVL
                     MQWLVAGAYFLDGFANAAEVWGGAAVGAGDRAALAAVARRTGELAFACALLLAGLFWL
                     AGDALIALITVSAEVRALADACLPWLVLSPIAGVAAFQFDGLFFGATRTAPLRDMMVL
                     AAGGYALALWWWLPRWGNHGLWAALMLFFVLRSVLLAACWPALKRSVGPA"
     gene            12017..>13011
                     /locus_tag="PK144_06160"
     CDS             12017..>13011
                     /locus_tag="PK144_06160"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNF65347.1"
                     /translation="MNPSIEHPVPPPPAPPEDYEAPVAAMHFRAIREDQPGERLAALY
                     ARSKPGYERWFLREGDAARPSWLACERALRHWLPELWPTFERVLAAVGDGDRTARFLS
                     LYQPTPFLAGCSQAAWARESVALVRNYDYSPRLADGVLLYTHWNSTPVIAMSDCLWGV
                     LDGMNGHGLAVSMAFGGRRAVGDGFGITLILRYVLEFCRDVPDALAVLTRVPVHMAYN
                     VALVDRGGQHATVYLGPDRPPLVSQERVSTNHQGSIEMPDHARTWETVRRDEYLRFCV
                     DAPYQNADALTGHFLVPPLYRNGHVRGWGTLYTAAYHPHAGTMALHWPQAGSWWQG"
BASE COUNT         1737 a         4486 c         4725 g         2063 t
ORIGIN      
        1 tcgtcaggca gatgaagcgt aacgtgccgt cggacagcat gtgcgctttc agtggcgtgt
       61 cggggtcatt gtattcggtc cattcgagct gggtcgactc gccgaccggc tcgcggtgta
      121 cgaagtcgcc gaaaaaaggt gcggcaaggc ggatcgtctc cacgatacgc agatagaaat
      181 ccggatagtc tttgcgaagt cgcaacagga atgcggcgag gttcgctgcg tcatgaagca
      241 gtcgcaggtt gatgtgggta tggtggatct gcttgatgcg ggcgttgtcg ctggtgtcgt
      301 gaaaatgaaa aacacggctg tcgataatgc ttttgttgac cagttggatg ccggttgagc
      361 ttgatgcgcg tttgatcccg gtttcggcgt ggccaacgcc aaggcggcgg ttcacgctgc
      421 cggctacaat acgttcatcc gaaaaaatga gcctgttgtc cgcagtcgct tccaattgag
      481 caaaatatcc gtacttgtcg agttcgacca agtactccat gtgttccgta tgcttgcggc
      541 cgcgatgcaa taatgcatcc ggcccgccct gcttttgaac gtacagttgc agccgccgtt
      601 cggacacggc tgtcagaaga cggaacaggc tgatgaagtt gcttttgccg gcgccgttgg
      661 cgccgatcaa gacattcagg tcacgcagtt cgaaggcttc gagtgcgcga atcgatttgt
      721 agccgcgaac cgtgattctg gtgatcttgc tcatgtcgga cagtgtggtt gatcagatca
      781 ggggcgtcac ggctggctgc gccccgccat gaactccgcc agcaccgccc ccgtatgcga
      841 ggcccccgcc tgcgccatca ccgcttccgg cgtgccctgc gcgaccagtt cgccgccggc
      901 ggcgccgcct tcggggccga ggtcgagcag ccagtcggct tcggcgatca gatccaggtt
      961 gtgctcgatc agcacgacgg tgttgccggc gtcgaccagc cggtgcagca cgcgcgcgag
     1021 cttgtcgaca tcggccatgt gcaggccgac ggtcggttcg tcgagcacgt acaaggtgtg
     1081 cggcgtcgtg cggccggtgc gcaggctggt cttgaccttc gccagttcgg tcacgagctt
     1141 gatgcgctgc gcctcgccgc ccgacagcgt cgggctcggc tggccgagcg tgaggtagcc
     1201 gaggccgaca tcctgcatca gcttcagcgg gtggtggatc agcgggtgtg cggcgaagaa
     1261 ttccacggcc tcgtcgacgc tgagtgccag caccgcgccg atcgacaggc cgcgccattc
     1321 gacctccagc gtcgcggcat cgaagcgcgc accgccgcag gcctcgcacg gcagcttcac
     1381 gtcgggcagg aagttcatct cgacgcgcac cgtgccgctg ccctcgcaga ccgggcagcg
     1441 gccgccggcg gtgttgaagc tgaagcgcga ggcgctccag ccgcggatgc gtgcttcccg
     1501 ggtgtcggcg tagagcttgc ggatcgcatc ccagaagccg acgtaggtcg ccgggcacga
     1561 gcgcggcgtc ttgccgatcg gcgtctggtc gacttcgagc acgcgcagga ccggctccca
     1621 gccgcgcagc agcgcgcagc cgagcagctc gatggcgacc ggctgcgcgt tgcggccctt
     1681 gccgcgcgcg gcgaccagcg cgtgcaggtt ggtgtaaagc acgtcacgcg ccagcgtcga
     1741 cttgcccgat cccgacacgc cggtgacgac caccagccgg ccgagcggca cgcgtgcgca
     1801 gacgttcttc aggttgtggc ggctggcccc gagcagttcg atcgccggct gcccgggctc
     1861 gaccggccgg cgcggctgca gcgggtgctg cagcggtgcg gccagacagc gaccggtcgg
     1921 cgaggccgga ttcgcggcca gttcatcggc cgtgccctcg gcgaccacct cgccgccgag
     1981 ccggccggcg ccggggccga ggtcgatgat gtggtgggcg cggcggatgg tgtcctcgtc
     2041 gtgctcgacg acgatcagcg tgttgccgcg cgcttcgagc ttttcgagcg catcgagcag
     2101 gatgcggttg tcgcgcggat gcaggccgat ggtcggctcg tcgagcacgt aggcgacgcc
     2161 ggacaggctc gatccgagct gcgcggcgag gcggatgcgc tgcgactcgc cgcccgagag
     2221 cgtcggggcg gcgcgatcga gcgtcagata gccgagcccg acctcctgca ggaagccgag
     2281 gcgcgaaccg atctcggcga ccaggccacc ggcgatgtcg gcctcgcgcc cggcgagcgc
     2341 cagttcatcg aggaaaccgg cgaccgccgc gaccggccgg gcggtcagcg caccgatcga
     2401 caggccgcgg aagcgcacgg cgcgcgccac cggattcagc cgctcgccgg cgcagtccgg
     2461 gcagggcgtg tcgtcgccgc gcgcatccag ccagtcatcg agcgcgcgat cgacctgttc
     2521 ctcgctcagg cggtcgtcgc gctggaagcc ggcgacgcgc acgccggtgc cctgacagcc
     2581 ctcgcaccag ccgtgcttcg agttgaacga gaacagccgc gggtcgagtt ccggaaagct
     2641 ggtgccgcag gccgggcagg cgcgcttcgt cgaccagacc tgcagcgcct cgccggccag
     2701 cacgtgcagc acgcccttgc cgatcgcgag cgcctcggcg agcgcggcgc gcagttcgcg
     2761 ttcggtgttc ggcagcaccg cgagttcggc gaccggcagc tccagcgtgt gctcgcggaa
     2821 gcgatcgagc cgcggccagg gtcgggtcgg cagccagtcg ccgtcgacgc gcaggcgctc
     2881 gtaacccttg ccgtgcgccc acttggcgag gtcggtgtac aggcccttgc ggtgcaccac
     2941 cagcggcgcc agcagcgtga tgcgcgagcc ccggtgctgc gccatcagct tcgcggcgat
     3001 cgcatcctcg ctctgcggct cgatcgggcg cgcgcagtcc gggcagtact gcgtgccgag
     3061 cttcatccac agcaggcgca ggaagtgata ggtctcggtc agcgtcgcca ccgtcgactt
     3121 gcgcccgccg cgcgacacgc gctgttcgat cgcgaccgtc ggcgggatgc cgtgcacggc
     3181 atcgacctcc gggcgcgccg ccggctgcac gaactggcgc gcgtaggcgt tcagcgattc
     3241 gagatagcgc cgctggccct cgccgaacac gatgtcgaag gcgagcgtcg acttgccgct
     3301 gccggacggg ccggtgatca ccgtgaagcc gcggtgcgcg atctcgacgc tgacgttctt
     3361 caggttgtgc tcgcgggcgt gatggatgcg gatggcgtcg ggcggggcgg ccgcgtagct
     3421 cgccggtgcc tcggcgacgg cgtcggcgcg gccttcggcg agccgctgca tgtcggcgcg
     3481 gtactgcgcc agcgcccgtc cggtatgcga ggcggcgcag gcgatcacgg catccggcgg
     3541 gccggcgcac agcagctcgc cgccgtcgtc gccgccttcg gggccgaggt cgagcagcca
     3601 gtcggcggcg tcgatgacat cgaggttgtg ctcgatgacc agcagcgtgt ggccggcatc
     3661 gagcaggcgt ccgaaggcct ggaggagccg ggcgatgtcg tcgaagtgca ggccggtggt
     3721 cggttcgtcg aacacgaaca gcgtctgcgg ctcgggctcg tcgccgcggc gccggcgctg
     3781 ccggccggcg gattcgacga ggtggccggc gagcttcagg cgctgcgcct cgccgcccga
     3841 gagcgtcggc accggctggc cgaggtgcag gtaatcgagc ccgacctcgg cgagcggtgc
     3901 gagcaggcga acgacctcgc ggccgccgcg cagcgggccg aagaagtcga tggcctcggc
     3961 gacgctcagg tcgagcacgt cggcgatgga ttttccggcc agttcgacct cgcgcacctc
     4021 ggcgcgaaag cgcgtgccgt cgcagtccgg gcagcgcaga tagacatcgg acaggaactg
     4081 catctcgacg tgctcgaagc ccgagccctg acagcccggg cagcggccgt tgccggcgtt
     4141 gaagctgaag gtgccgggcg tgtagccgcg ctcgcgcgcc gcgtcggtgc cggcgaacag
     4201 cttgcggatc gcgtcgaagg cgccgatgta gctcgccggg ttcgagcgcg cggtcttgcc
     4261 gatcggcgac tggtcgacga cgacgacctc gccgatctcc agcccgcctt cgagcgcccg
     4321 gaacgcgccg ggcgcctcgg tcggcctgcc gagccggcgg gccagcgccg gcaccaggac
     4381 ttcctgcacc agcgtcgact tgccggagcc ggagacgccg gtcacgcaca ccagccgtcc
     4441 gagcgggatc gcgacatcga tggccttcag gttgtgctcg gccgcgccgc acagccgcag
     4501 ccgcggcgtg ttgtcatcga ccggtcgcgg cacgcgcccg gcggcgacgt ggcggcggcc
     4561 gagcaggtag tcggcggtca gtgaaccgcg cgctccggcc agccgcgccg gggcatcgaa
     4621 gaacatgacc tcgccgccgc gcgcgccggg gccgggaccg aggtcgagca gccggtcggc
     4681 cgcaaacatg atctgcggat cgtgctcgac caccaccagc gtgttgccct gcgcgcgcag
     4741 gcgctgcatc accgcgatca cgcggtgcat gtcgcgcggg tgcaggccga tgctcggctc
     4801 atcgagcacg aacagcgtgt tgaccagcga ggtgcccagc gccgtggtca ggttgatgcg
     4861 ctgcacctcg ccgccggaga gcgtccgcga ctggcgatcg agcgtcaggt agccgagccc
     4921 gacctcgccg aggaagcgca ggcgcgtgcg gatctcgccg agcaccagtc cggtggcctc
     4981 gtcgagcggt gccggcagcc gcagctgcgc aaagaagctc gcgagccgcg tcagcggcag
     5041 cagcatcagg tcgtgcagcg tcaggccggg cagggtttcg agcacggcgc gccggtagcg
     5101 cacctgcgtc ggcaggaagc gtcgcgccgg cgccagcacc gcgtcggcct cggcgcgcgt
     5161 gccgaggcgg aaatccagcg attcgggctt cagccgcgcg ccggcgcagg ccgtgcaggg
     5221 cgtgtaggcg cggtacttcg agagcagcac acggatgtgc atcttgtacg ccttgctctc
     5281 cagccagtcg aagaagtggc gcacgccgta ccagagcttc ggccacgagc gctcccagct
     5341 ctgccagccg tcctcgcctt cgagcaccca gtgccggtgc tcgggcggca ggtcgcgaaa
     5401 cggcacgtcg agcgcgaccc cgcgcagccg cgcgtacttt tcgagatcct gctggcactc
     5461 gcggaagctc tcggtctgcc acggcttcac ggcgccctgc gcgagcgtct tcgattcgtc
     5521 gggcacgacc agcccgaaat cgacatcgat gacccgcccg aagccgcggc agatcgggca
     5581 ggccccgagc ggcgagttga acgagaacgc cgccggcacc ggctcggcgt aatggatgtc
     5641 gcagtccggg cagtgcaggt cggccgaata gcgccagcgc ggggcgggcg cttcgggcga
     5701 gtcgccgtcc ggagcgggtg cagggtcgag cacatgcacg tcgacccgcc cgcgcccctg
     5761 acgcagcgcg gtttccagcg cctcgatcac gcgcgcccgt tcggccgtgc cgagccggaa
     5821 gcggtcctgc acgacgtgca gccagccggg ttcgcgggcc tggatgcgcg tatagccctg
     5881 ctgcgcgagc agcgcctcga catcggcccc ggcctgatcg cggacggcga ccgggaagct
     5941 cacggccaga cgcggatcgc cggccgcggc ggcgcgggcg tgcaggtcat cgaggatgct
     6001 gtccggcccg tcgcggcgca ccggccggcc gcagccgcgg cagcacggcg tgctcatgcg
     6061 cgcgtacagc agcttcagat ggtcgttcag ctcggtcatc gtgccgaccg tcgagcgcga
     6121 ggtgcgcacc ggattggtct ggtcgatcgc gatcgccggc ggaatccccg ccaccgagcc
     6181 gacctgcggc cggtcctgcc ggtcgaggaa ctggcgcgcg taggggctga aggtctcgac
     6241 gtagcgacgc tggccttcgg cgtacagcgt gtcgaacacc agcgaggact tgcccgaacc
     6301 cgagacgccg gtgacgacgg tcagccggcc ggtcgggaag tcgaggtcga ggtgcttgag
     6361 gttgttctgg tgcgcatccc gcagccggat cgcgccggtg gcgggcacga cgggcgtgcg
     6421 gggatcggtc atcgggggga tcagtcggcc agcaggttgc ggaacggggc ggtgttcttc
     6481 cagcgatagg tgccgaaatc gcgcatgtcg gtggacagga tgcggccgtc gcccagatgt
     6541 tccgcgagca cgaccaggga ggcgtcggcc aggtccatcg gcaggttggc gtagcgctgc
     6601 atgagttcga tgacacgcgg caaatggatg tcgcgcaggt cgaagatatc gaggactccg
     6661 gtggcgaggt gctggaggaa caactgctgc gcgtgccggc tgacgcgctt gcccagcaga
     6721 tggcaggttt ccgtgatcac cggccaggtc acgatcagcc gctccttcag gtccgcgagc
     6781 tggcggcacg cccgttcatg aaaacggtcg tccggcatgg ccagcgcgat ccagaaaccg
     6841 gtgtcagcga tgatcatgtc cgtcttcgac gatggcggcg aatgccttct tgtattccgt
     6901 ggaaagctcc gtctggcctt gcgggccgca gccgatgaag cctgagtcca gcagggcctg
     6961 atacgtcgac ggctgcccgg tcacgagccg gtcgtgatac aggtcgagcg cctgctcgat
     7021 tacgtggctg atgctggcgc cggtcattcg ccggatttcg tcgagcttgc gggcgtgcgc
     7081 ttcatcgagt ctggactgga tgcgcatggc atgactccgg gggcaggcgc gaatggacgg
     7141 ccagcctagg cccggccgct tgcgtccggc aatgccggtt ccggtatccc ggccatggcc
     7201 tgccggcagg cgtgaatgtg tccggttgcg tcaggcgatt ttacaaacca tgtcgggttc
     7261 aggatgcggt ggttgagata attgctttca tggcaatgac ttgattaatt ggctcgggta
     7321 ttgcttggat tcactgtgtc tgtccaaaag ttcactggac cctgcccggc gtcattgagg
     7381 aacccggatc gttcacgccg ttccccccac cgatcccggc ccgaaccatg tcgcacgtcc
     7441 tgtcccgaat cccgagcttc aatgccctgc gcggcctgtg cgcgctgctg ctgctggcgg
     7501 cgagcgtgtt cgcccatgcc ttcgggcttg gtctgggcgg cccgtccgac accgaaccgg
     7561 ctgcccgcgc cggctggtgg tatgccgcac cgggggtcga gacccgcttc gagctgcagg
     7621 ccggcgagac gcgcatgctg ccgctcggcg agctggtcgt cggggccttc gacagcgccc
     7681 gcgtgctggt gccgggcgtg ccggccgacg aggaccgcta ccggctcgaa ttcgagctgc
     7741 agcccgaggg cagtgcaccg gtgcacatca gcgccagcgc cgtggtcggg cgcgccggcg
     7801 atgcgcacgg cgagcggctg accttcgact tcggcgccga gccggtgcag atcggcttcg
     7861 gccgcggcgg cgtgctcagc gtgcatttcc ggccgctcga cctcgccgac aacggccgct
     7921 acgacctgta tgccgaagtc cggctggtgt cgccgccggc cgagctgccg gagccggacc
     7981 tggtctggca gctcgggctc gggctggtgc tgctcgcgct ggtccgccac ctgcgccgcc
     8041 gttcctgaaa tcttctcaaa tctgtctcgc acaggccgca gccggagcgg acagcgacaa
     8101 tactgcgcgc agcttcccgt tccccggagt gcgccgatgc ctgccgaact cgaaaccctg
     8161 atccgtcagt tgcgggaaga tcgtgccgcc ggcgccatcg agctcgcccg tcaggcgctt
     8221 gccggactcg gggcctatgc cgagcgcgtg ccggcgcgct cggtgccggg gctgcgcgcg
     8281 agtctcgccg aggcggcgat gcaggtgacg gcatcgcggc cggccgtgac gccgctgatg
     8341 acgctggtca acgactggca cggacgcatc gagcgcgtcg aggccggcac gctgcaggcg
     8401 ctgcgcgcgc atgccgtggt cagtgccgcg cgcctgagcg agcactcgcg cagcgccggc
     8461 gcgagcaccg ctgccaacgt cgctgcctgg atcgggccgg ggcgtaccgt gctgacgcac
     8521 ggctggagca cgacggtgct cgcctgcctc gcgcagatcg ccgcgctcgg cggcctgcgc
     8581 gtgatctgca ccgaggcgcg cccgctgaac gagggccgcc agctcgcggc ccgcgccgcc
     8641 ggtctcggcg tgccggtgac gctgatcacc gatgcgcagc tggcgctggc ggcgaccgcg
     8701 gccgacgtgg tgctggtcgg cgccgacacc gtgctcgtcg acggtgccgt ggtcggcaag
     8761 gccggcacct ggccgatggc gctggcggcg cgcgccgtcg acattccgtt cgtggtcgcc
     8821 gccgagtcct gcaggcgcag tccggaactg agcgtgtcgc tcgaagacca ggacccggcc
     8881 gaggtcgggg cgccgctgcc gggcgtgatg ctgcgcaacc gccgcttcga cgtgacgccg
     8941 ccggatctgg tcacggcctg gatcgacgag cacggcgtct ggaccggcga ggcggcgtga
     9001 agcgcgcttg ccagcggctc acgggccggc tcgcggggct gcgggggcgt ggctagactg
     9061 ccgctccctt ttcgtgaccg gagccgcccg caatgaccgg catccatgat ttccctgcct
     9121 tcctgctcgc ttcggtggcg ctggccgtgc tgccggggcc ggacacgctg tacgtgctcg
     9181 ggcgcaccgt cgcgcagggc cggcgcgccg ggctctggtc gctggccggc atcggcggcg
     9241 gctgcctcgg tcatgtcgcc gcctcggcgc tcgggctgtc ggcgctgatc gccgcgtcgc
     9301 cgttcgcgtt cgagctggtc aagctcgccg gtgcgctgta cctggcctgg atcggcgtgc
     9361 agctgctgcg cgaggcctgg cggcacgccg gcgacggtgt gccgctgccg gccgtggccg
     9421 cggacgaggc cggtggctgg cagctgctgc gtcagggcgt ggtgaccaac ctgctgaatc
     9481 cgaaggtcgg gctgttcttc atctccttcc tgccgcagtt cgtgacgccc ggagtcggca
     9541 cggtcgggga tttcgtgctg ctcggcctga ccttcgtcgg catcggtctg gtgtggatcc
     9601 tgttgctgat gcacacgacc gatctcgcga cccggcactt ccgcggccgg gccgccgtgc
     9661 agcgctggtt caaggccagc accggcggcc tgttcgtcgg gctggcggcg aagctcgcgc
     9721 tggatcgctg aactgctgcc ggagtcaccg ttcatggaac gcaaccacgc ccgtgccagc
     9781 gatggcgccg tgctcgccgc ctgccgccac ggcggccatc tgctgtcgtg gcagaccccg
     9841 gacggccgcg agcgcctgtt cctgagtccg ctcagccagc cggcgcccgg cgtcgcgatc
     9901 cgcggcggcg tgccggtgtg cttcccgcag ttctcgggct tcgggccgct gccgaagcac
     9961 ggcttcgtgc gcgatcggat ctggcagtgc acggcggccg gcaccgctgc tgacggccgc
    10021 gcgcggctga cctatgcact cgaagccgat gccgcgaccc gcgcgctgtg gccgcaggcc
    10081 ttccgctgcg aactcgaact gctcgcggcc ggcgacacgc tggccgtgac gctggccgtg
    10141 cacaacaccg gcgaggcgcc gctgtcgttc accggcgcgc tgcataccta cttcgccgtc
    10201 accgacctcg acgccgcccg cgtcagcggt ctggaagcgc atccgtatcg cgaccacgcc
    10261 ggtgcggcgg cagccggtgg tggcatcgac cattcgcccg atggcgcgcc gctttgcttc
    10321 ggcgccgaag tcgatcgcgt gtacgccggc gtgcgcggag cgatccggct cgcgaccggt
    10381 gccggcacgc tgcagatcga ggccgacggc tttccggacg ccgtgatctg gaaccccggg
    10441 ccggagaaag gcgcagcact cgccgatctc gaagccgggg cctggcgccg ctacgtctgc
    10501 gtcgaggcgg ccatcgtgcg cacgccggtg tcggtcgccg ccggtgcgct ctggcgcggc
    10561 acgcagcggg tccgcgtgat ttgacggacg ccgcgactgc cgtaccgtcg cgcgcggcga
    10621 tcctgcgctt cgcggcgccg gtgatgctcg ccaacctcac gacgccgctg ctcggcatcg
    10681 tcgacaccgc gatcatgggc cgttcgcccg atccggcgct gatcgccgcg gtcgcggtcg
    10741 gctcgacggt gctgaccgtg ctgttctggg ccttctcgtt cctgcgccag agcacggtcg
    10801 gcctgaccgc acaggcgctc ggcgccggcg atgccacgga gctggccgcg gcgctgctgc
    10861 gcccgctggc actggcggcg ctgatcggca cggtgctgat cctgctgcag gcaccgatcg
    10921 cggcgctggc cttcgggctg atgacgagtg ctcccgaggt cgaaaccgcg gcgcgcgcct
    10981 attacggctg gcgcatcgcc gccgcgccgt tcgcgcttgc caattacgca ctcgccgcct
    11041 ggctgatcgg ccagcgccgg gcgcgcctcg tgctcggcct gcagctggcg atgaacggcg
    11101 gcaacgccgc gctgaccgcg ctgttcgtgc tcggcttcgg tctcggcccg gccggggccg
    11161 gactcggcac cgcgctcgcc gagatgctga cctgtctggc gacgctgggg ctgccggccg
    11221 tgcgcgcgca gttcgcaccg gccgtgctgc gggcggtgtg cagcggcggg cgcgcgcagt
    11281 tcgcgcggct gatgcgcgcc aaccgcgaca tcctgctgcg cacgctggtg ctgacgctga
    11341 cgctcgcctc gttcacgcgc ttcggcggca cgctttcgac cgcgacgctg gccgccaatc
    11401 aggtgctgat gcagtggctg gtggccggtg cgtattttct cgacggcttc gcgaacgccg
    11461 ccgaggtctg gggcggggcc gcggtcgggg ccggcgatcg cgccgcgctc gccgcggtcg
    11521 cgcgccgcac cggcgagctg gcgttcgcgt gcgcgctgct gctggccggg ctgttctggc
    11581 tggccggtga cgcactgatc gccctgatca cggtcagtgc cgaggtgcgc gcgctcgccg
    11641 atgcctgcct gccgtggctg gtgctctcgc cgatcgccgg cgtcgcggcc ttccagttcg
    11701 atggcctgtt cttcggtgcc acgcgcaccg caccgctgcg cgacatgatg gtgctcgccg
    11761 ccggcggcta cgcgctggcg ctctggtggt ggctgccgcg ctggggcaat cacgggctgt
    11821 gggcggcgct gatgctgttc ttcgtgctgc gcagcgtgct gctcgccgcc tgctggccgg
    11881 ccctgaagcg cagcgtcggc ccggcctgaa accgcagccg gggcttgccg gcggcgcgca
    11941 ctgaaggcac aaccgtcgga tttcccctgt ccggccacgg agcggcccga tctgccgatc
    12001 gtcgaggatc gccgccgtga acccgagcat cgaacatccc gtcccgccgc cacccgctcc
    12061 gcccgaggac tacgaggcgc cggtcgccgc gatgcacttt cgcgcgatcc gcgaggatca
    12121 gcccggcgag cggcttgcgg cgctgtatgc gcgctcgaaa cccggctacg aacgctggtt
    12181 cctgcgcgag ggcgatgcgg cgcggccgtc gtggctcgcc tgcgagcggg cactgcgtca
    12241 ctggctgccg gagctgtggc cgaccttcga gcgcgtgctg gccgcggtcg gcgacggcga
    12301 ccgcacggcg cgcttcctgt cgctgtacca gccgacgccg tttctggccg gctgctcgca
    12361 ggcggcctgg gcgcgcgaat cggtggcgct ggtgcgcaac tacgactact cgccgcgcct
    12421 ggccgacggc gtgctgctgt acacgcactg gaactcgacg ccggtgatcg cgatgtcgga
    12481 ctgcctgtgg ggcgtgctcg acggcatgaa cggccacggc ctcgcggtct cgatggcctt
    12541 cggcggccgg cgcgcggtcg gcgacggctt cggcatcacg ctgatcctgc gctacgtgct
    12601 ggagttctgc cgcgacgtgc ccgatgcgct ggcggtgctg acgcgcgtgc cggtgcacat
    12661 ggcctacaac gtcgcgctgg tcgatcgcgg cggccagcac gcgaccgtct atctcggccc
    12721 ggaccggccg ccgctggtgt cacaggagcg cgtgtcgacc aatcatcagg gctcgatcga
    12781 gatgccggac catgcccgca cctgggagac ggtgcgccgc gacgaatacc tgcgcttctg
    12841 cgtcgatgcg ccctaccaga acgccgacgc gctgaccggc cacttcctgg tgccgccgct
    12901 gtaccgcaac ggccacgtcc gcggctgggg tacgctgtat accgccgcct atcacccgca
    12961 cgccggcacg atggcactgc actggccgca ggccggcagc tggtggcagg g
//
LOCUS       DAOQIE010000069        12949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2362_length_12949_cov_6.927175, whole genome shotgun sequence.
ACCESSION   DAOQIE010000069 DAOQIE010000000
VERSION     DAOQIE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12949
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_12949_cov_6.927175"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1120)
                     /locus_tag="PK144_06165"
     CDS             complement(155..1120)
                     /locus_tag="PK144_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNF65348.1"
                     /translation="MKIPVLPLLAVAALGFAVISISRTQPVHPLADPPSAPPVSPYAH
                     AIGAVGLVEPPGEAIAIGTHLPGVVERVEVVAGQTVAAGAPLFRIDPRPYASTLAVRE
                     AAAKAAEAAVRTAQALLADAKAQQAHVQKLADQRSIATEEVIRRRYALDEAGARYDEA
                     RAVAERAQAEVRDARTELERTTVRAPLDAVVLAVNLRAGEFAPAGVPAEPLLVLGARG
                     ALQVRVDIDEHEGWKLDPQRPAVARLRGNATISSPLTWVRTEPLAVPKRSLTGATTER
                     VDTRVVQAIYRIENAGFPVRVGQQLDVYVETAAPDIPGTAAEVRP"
     gene            complement(1117..1842)
                     /locus_tag="PK144_06170"
     CDS             complement(1117..1842)
                     /locus_tag="PK144_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65349.1"
                     /translation="MSAPATPAVRTQALTRSFGDGDESVQVLRGVDYQAGFGALNYLV
                     GPSGCGKTTLLSIVAGLLEPGDGDLEVLGERPQRFGARARAAFRRERLGFVFQQYHLL
                     PALTAAENAAVTLIAAGMARTRAVERARALLHELGLGGRADALPKHLSGGQQQRVALA
                     RALVHEPQLVLCDEPTAALDAESGRRVVELLRAVAVRPDRAVIVVTHDNRIFDFAGRI
                     DHMEDGRIVKSESRMPAAPETSA"
     gene            complement(1839..2978)
                     /locus_tag="PK144_06175"
     CDS             complement(1839..2978)
                     /locus_tag="PK144_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF65350.1"
                     /translation="MNYIAWQMLTGDRAKYFALVFTIAFASFLLVQQVSIFVGILERT
                     ASQIVDVAEPDLWVMDPATRYFDEVQALKSNDLYRVRGVPGTQWAVPLYKGNARMRAA
                     DGSFRQVILLGLDDATLTGVPRRMLLGSAGALAEPDAVILDRAGYLFFFPGEPLVLGR
                     EFELNDRRAKLVGIAEASAPFVTFPVVFTRYSNAVTYIGRERNTLSFVLAKAAPGVPV
                     AELAARIGAQTGLAAQSVDGFRRQTIRFYLANTGIPVNFGITIVLAVLIGMVVAGQTF
                     YLFTLENLKQYGVLKAIGVSDGRIVAMILLQACVVGLIGYGLGMGLVAAFFEVMLGYL
                     PVRGIVVQPVAAMVTGAVVLAIVALASLLSVRRVLVLEPAAVFRG"
     gene            complement(3157..3888)
                     /locus_tag="PK144_06180"
     CDS             complement(3157..3888)
                     /locus_tag="PK144_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF65351.1"
                     /translation="MTAPTQTRKARERAAREQLILDHARHMLAAQGYLDLNLDELARR
                     VEYSKGTLYQHFSSKEDLVMAIAVQGMELRAGLFERAAAFRGSSSRERIQAFSVAAAQ
                     FSHEYPDYFEVETLVKIASFAGRASEERQNALGLGVGRCFRAVNAVVHAALAAGDLPA
                     DGLPPERIVFAIASVSIGSEIMGRLPALRLLAGIDELAWTVRANQCVLLDGLGWRPLS
                     RDFDMAAVDRRLRAEVFPEATWLQV"
     gene            4195..5250
                     /locus_tag="PK144_06185"
     CDS             4195..5250
                     /locus_tag="PK144_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF65352.1"
                     /translation="MDHRFAASRSASLAVLAAFAGAAMLLLLGACEREAPQTRSAALT
                     QNAPPPVPRPSETGGPQVTLVMKTLTNPFFVDMERGARRAATETGARLLVRAAAQETS
                     IDQQIEIVETAIRERTDAIVIAPGDSAALIPVLRKARDAGIRLVVIDNPLDPVLAERA
                     GLGDVPLVTVDNERGAALAADQAAASLQGPVPTLLLEGIRGAANAEARRRGALSALAR
                     HPAVQVVASESAHWKIDEAFEVAQREFSEHPELRLVVCANDMMALGVIRYLKDAGRPD
                     VRVTGYDALPEAREALAQGWLYATVDQRAAEQGETGVRYALRALAGEVLPARTLVPVT
                     LVLRPDGGGADGRTGER"
     gene            5240..7705
                     /locus_tag="PK144_06190"
     CDS             5240..7705
                     /locus_tag="PK144_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65353.1"
                     /translation="MNADPRRWLRRATLAWRIGLYLAAASAVPLLLTGYLSYHMAADA
                     LLREATHSVAAAVEAQRRYIEAQMAQVESLLANLAGVEAITAAAIEEQSDADTYDRLA
                     TQARIGYILNGYSSLRGLVSIDLIGAGGRHYHVGDTLEAVRLDEEQRRRLFEAAQRSG
                     RTVHWSGIGPNINVHSAYRKVITAARLLYRVERETLRREPVALLLVSYDPDELARQFD
                     DVALGNGAWFVAVDGDGRLLYHPDARLRGERLATPLLETLRDAQGSRSVNIAGHDYLL
                     AHARAVTLGWTIAGFVPLDTLAAPVAPIRWASFGLVAGCLLFALLMAIRLHAKVIAPL
                     RAVIRQLQRFERDPAGAEHLGITNDDEIGELTRWYNRSLDAVHALRDSEERAQHAALH
                     DVLTGLANRTLLIDHITHAQAAVRRQPERPCALLFIDLDHFKPVNDRLGHEAGDKLLC
                     AIAQRIGDCVRSIDVLARLGGDEFAVLLTRLDEAGRALRIATRIVECVRRPVTIDGHE
                     VQVGASIGIAIGDGTQDAEALLRDADIAMYRAKQTGKSRCELFDAALGAQVLERVGLE
                     FELRRALERGQLAMHYQPIVALADDRIVACEALMRWQHPELGAVAPDRFIPIAEETGL
                     IAELGAWALASACAQAASWRARWGEAAPAIAVNLSPRQLGRPDFCEQVAACFAEHGIG
                     PGMIHLEITETALLADLGHADRLLACLRGLGATIHLDDFGTGYSSLSYLSRLQVDAIK
                     IDRAFVRRLDPAHDGPDLVRAILLIADELGLTVVAEGIETEYQHQRLAALGCAHGQGW
                     RFGRAAEPAAIETQLRRQLVRTG"
     gene            complement(7726..8187)
                     /gene="cueR"
                     /locus_tag="PK144_06195"
     CDS             complement(7726..8187)
                     /gene="cueR"
                     /locus_tag="PK144_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HNF65354.1"
                     /translation="MNIGAAARASGVSERMIRHYEKLGLLAPAARTAAGYRTYTAGDV
                     HVLRFVRRARDLGFPVADIARLLALWQDRSRASAEVKRLAAGHIAELDARIASLSAMR
                     DALARLAAACCGDARPDCPILDELAGDTPLPQPRPPEDAGPVTRTPGSRRR"
     gene            complement(8213..11407)
                     /locus_tag="PK144_06200"
     CDS             complement(8213..11407)
                     /locus_tag="PK144_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNF65355.1"
                     /translation="MNPSARTPDAVRLGELCAQIETVMLARQDPVTGLLPASTAVTVH
                     GDYTDAWVRDNVYSILCVWGCGLALRRHRGGDPRAYRFEQGAVKTMRGLLTAMMKQAH
                     KVERFKHTLAPIDALHAKYGSRTGEPVVADDGWGHLQIDATSVFLLMLVQMIASGLRV
                     VYSLDEVDFVQALAHYIARAYRTPDYGIWERGDKINTGDPEINASSVGMAKAALEALR
                     GFDLFGAEGGPASVIHVSSDDIARMRNLLEATLPRESRSKEVDAALLSVIGFPAWAVE
                     DAELVERTRHEVVAKLQGRYGCKRFLRDGHQTVLEDHTRLHYESGELARFERIESEWP
                     LFFTYLMLDGLMRGRDGQAHEYRERLAPLLVDRGAGPLLPELYLVPEKSVAAEKARPG
                     SQPRLPNANVPLYWAQSLYWLALLIDEGWLHPGDIDPLNRRHRIGRRRECTVQVALIA
                     DTAAVETALLDAGVRAQTPEAVLPVRVRQASELVEAMTNVGRNAALGLSGRPRRGVGT
                     LVTCQVFTIGGETVVFLPQFLERQDFYLNLSNRVLISRCKAEFAHVRRHWDQAGQPVF
                     ALRLTARMLATDGAGELLDFLRALCAGSCEGLPVRVRPLSELIATAGRKRFDRLRDYH
                     FEFAPLEQERSAGNLLDIDPAAARELDAADLQRLEDLPPPAVPVRLAATRNLHEVIAL
                     LEIAARRDGIDAPLPDGRSVRACLEAAYALAAEAGHWGLLRRCAGLLDKHDPKLEDAV
                     ANIVAHRKQIALGRGYSDGSVVSTALGNREIVACLRQYAGDDPAGRMLVQEVVLAVET
                     LLKTDPAVFRDTMTIRAWPLVLLVVGDYAWEHRLSQPEAFRRVQTLGPWAFMGRVQAV
                     VRGDNPVSRLARLESLRRDDAAHGLAVPDPQEDAGAEAVVDWLAWRRRHGVLTQLSAH
                     FYEQVWAVLAHCEGLVLGERLDIGNCLDSARLRADMTAAETNFALTVNRLLDKIQSPE
                     YRQLNIEALAALAHLCSANPGLHVAGHIVIDVLTGHAVRLHWLQQHPDHAGHYEAFRA
                     EAWSALYASPPAEVESALIGAFAHLLGEARPAAA"
     gene            11660..>12949
                     /gene="glgB"
                     /locus_tag="PK144_06205"
     CDS             11660..>12949
                     /gene="glgB"
                     /locus_tag="PK144_06205"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005290558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNF65356.1"
                     /translation="MSVAPSPDIVQAIVTGLWPDPYPKADPFAVLGMHVEAGAGVVVR
                     SFQPDAESVEVLDAASGRRVATLERIDAAGLFAGSVARRKNPFAYRLRVRYAGTVVEL
                     DDPYRHASSLGELDLHLLGEGRHWRAFEALGAHCLRLAGSDGVRFAVWAPNARRVSVV
                     GDFNRWDGRRHPMRFHPGSGCWELFIPGLAEGVLYKYELLGPDGTPLPLKADPCGYAA
                     ELRPDTASRVARLDHHAWNDGDWMATRAARIARDAPVSIYELHLGSWRRSPDDPDRPL
                     GYRELADALAPYLNDLGFTHVELLPVTEHPFDGSWGYQPLGLYAPTCRHGSPADFQYF
                     VDRLHQAGIGVLLDWVPAHFPADAHGLARFDGTALYEHEDPRLGFHPDWNTLIYNLGR
                     HEVANFLIANALFWLERYHLDGLRVDAVASMLYLDYSR"
BASE COUNT         1848 a         4678 c         4633 g         1790 t
ORIGIN      
        1 ggtgcagata acgatgctga atcctgcctc gaatcagatg cagcaagaaa cgtcgcccct
       61 gactgcccct ctccccttgc gggagagggg ttggggagag gggtaagccg atggcactga
      121 gccggaccgc aagcatccat gtgtcgatat ttactcatgg cctgacttcc gccgcagtcc
      181 ccggaatatc aggcgccgcc gtctcgacat acacgtcgag ctgctgcccg acccgcaccg
      241 ggaaaccggc gttttcaatc cggtagatcg cctgcacgac gcgcgtgtcg acgcgctcgg
      301 tggtcgcgcc ggtcagcgaa cgcttcggca ccgccagcgg ttcggtgcgc acccacgtga
      361 gcggcgacga gatcgtcgca ttgccgcgca ggcgcgcgac ggccgggcgc tgcgggtcga
      421 gcttccagcc ttcgtgctcg tcgatgtcga cgcgcacctg cagcgcgccg cgggcaccga
      481 gcacgagcag cggttcggcc ggcacgccgg ccggcgcgaa ctcgccggcg cgcaggttca
      541 cggcgagcac gacggcatcg agcggcgcgc gcaccgtcgt gcgttcgagt tcggtgcgcg
      601 catcgcggac ctcggcctgc gcgcgctcgg ccaccgcgcg tgcctcgtcg taacgggcgc
      661 cggcctcatc gagcgcatag cgacggcgga tgacttcctc ggtcgcgatc gaacgctggt
      721 cggcgagctt ctgcacgtgc gcctgctgcg ccttcgcatc ggcgagcagc gcctgcgcgg
      781 tgcgcacggc cgcctcggcg gctttggccg cggcctcgcg caccgccagc gtgctcgcgt
      841 acgggcgcgg gtcaatgcga aacagcggcg ccccggcggc gaccgtctgc ccggcgacga
      901 cctcgacgcg ctcgacgacg ccgggcagat gcgtgccgat cgcaatcgcc tcgcccggcg
      961 gctcgaccag cccgaccgcg ccgatcgcat gcgcgtacgg gctgaccggc ggtgccgacg
     1021 gcggatcggc gagcggatgc accggctgcg tgcggctgat cgatatcacg gcaaagccga
     1081 gcgcggcgac cgcgagcagc ggcaggacgg gaatcttcat gcggaggtct ccggagcggc
     1141 cggcatgcgc gattcgctct tcacgatgcg gccgtcctcc atgtgatcga tgcgcccggc
     1201 gaaatcgaag atgcggttgt cgtgcgtgac gacgatgacg gcgcgatccg ggcgcaccgc
     1261 gaccgcgcgc agcagctcca cgacccgccg gccggattcg gcgtcgaggg cggcggtcgg
     1321 ctcgtcgcag agcacgagct gcggctcgtg caccagcgcg cgcgcgagcg ccacgcgctg
     1381 ctgctggccg ccggacagat gcttcggcag cgcgtcggcg cgcccgccga gaccgagttc
     1441 atggagcagc gcgcgggcac gctcgacggc gcgggtgcgc gccatgccgg cggcgatcag
     1501 cgtcaccgcg gcgttctcgg ccgcggtcag cgccggcagc aggtggtact gctggaacac
     1561 gaagccgagc cgctcgcggc ggaaggccgc gcgggcgcgg gcgccgaagc gctgcggacg
     1621 ctcgccgagc acttcgagat cgccgtcgcc gggttcgagc aggccggcga cgatcgacag
     1681 cagcgtggtc ttgccgcagc cggacgggcc gaccagatag ttcagcgcgc cgaatccggc
     1741 ctgatagtcg acgccgcgca gcacctgcac cgactcgtcg ccatcgccga agctgcgcgt
     1801 cagcgcctgc gtgcgcacgg ccggggtcgc cggcgcgctc atccgcggaa caccgcggcc
     1861 ggctccagca cgagcacgcg gcgcaccgac agcaggctcg cgagcgcgac gatcgcgagc
     1921 acgacggcgc cggtgaccat ggccgcgacc ggctgcacga cgatgccgcg caccggcaga
     1981 tagccgagca tgacctcgaa gaaggccgcg accaggccca tgccgaggcc gtagccgatc
     2041 aggccgacca cgcaggcctg cagcaggatc atcgcgacga tgcgcccgtc cgagacaccg
     2101 atcgccttca gcacgccgta ctgcttcagg ttttcgagcg tgaacaggta gaaggtctgg
     2161 ccggcgacca ccatgccgat cagcaccgcc agcacgatgg tgatgccgaa gttgaccgga
     2221 atgccggtgt tggcgagata gaagcggatg gtctggcgcc ggaagccgtc gacgctctgc
     2281 gccgccagcc cggtctgcgc gccgatgcgc gcggccagct ccgcgaccgg cacgcccggc
     2341 gcggccttcg cgagcacgaa ggacagcgtg ttgcgctcgc ggccgatgta ggtgacggcg
     2401 ttcgaatagc gcgtgaacac caccgggaag gtcacgaacg gcgcgctggc ctcggcgatg
     2461 ccgacgagct tcgcgcgccg gtcgttcagc tcgaactcgc gcccgagcac gagcggctcg
     2521 ccggggaaga agaacaggta gccggcgcga tcgaggatca ccgcatccgg ctcggccagc
     2581 gccccggcgg agcccagcag catgcgccgc ggcacgccgg tcagcgtcgc atcgtcgagg
     2641 ccgagcagga tcacctgccg gaagctgccg tcggcggcgc gcatgcgggc gttgcccttg
     2701 tacagcggca cggcccactg cgtgccgggc acgccgcgca cgcggtacag gtcgttcgac
     2761 ttcagcgcct gcacctcgtc gaagtagcgc gtcgccgggt ccatgaccca caggtccggt
     2821 tcggcgacat cgacgatctg actggccgtg cgttcgagga tgccgacgaa gatcgacacc
     2881 tgctgcacga gcaggaagga cgcgaacgcg atcgtgaaca ccagcgcgaa gtacttggcg
     2941 cgatcgccgg tcagcatctg ccaggcgatg tagttcatgc cggtgacccc gcggtacttg
     3001 ctgcgcctgc tgcactgaat gatatttttc gactttcagt caaatcatga gcacacgaaa
     3061 gcccgccgga caccgccggc cggaccgggc cggacgcagc ggatgcccaa gggcgcagcg
     3121 gccccgggag aaggcaggga tgcggattgc gcggactcag acctgcagcc atgtcgcctc
     3181 cggaaacacc tcggcgcgca ggcgccggtc gacggcggcc atgtcgaagt cgcgcgacag
     3241 cggccgccag ccgaggccgt cgagcagcac gcactggttt gcgcgcacgg tccaggccag
     3301 ctcgtcgatg ccggcgagca gccgcagcgc cggcagccgg cccatgatct cgctgccgat
     3361 gctgaccgag gcgatcgcga acacgatgcg ctccggtggc aggccgtcgg ccggcaggtc
     3421 gccggcggcc agcgccgcat gcacgacggc attgacggcg cggaagcagc gtccgacgcc
     3481 caggccgagc gcgttctggc gctcctcgct ggcgcggccg gcgaagctcg cgatcttcac
     3541 gagggtctcg acctcgaagt aatccgggta ctcgtggctg aactgtgcgg cggcgaccga
     3601 gaaggcctgg atgcgctcgc ggctgctgct gccgcggaag gccgcggcgc gctcgaacag
     3661 cccggcgcgc agttccatgc cctgcacggc gatggccatg accaggtcct ccttggagct
     3721 gaagtgctgg tacagcgtgc ccttcgagta ctcgacgcgc cgggcgagct cgtcgaggtt
     3781 caggtcgaga tagccctgcg cggcgagcat gtgccgggcg tgatcgagga tcagctgctc
     3841 gcgcgcggcg cgttcgcggg ccttgcgggt ctgggtgggc gcagtcatgc atgaactctg
     3901 gtccattttt gactgatggt caatttaaga tcagcgtctt ccggccggaa ccggctccgg
     3961 caccggttgc cacccgcggg ccacgcgacg gcgcacaccg gagaggcgcc cgcgcgctgc
     4021 cgtcacgtcc tgaacgaacc gctgcggccc cgagcggccg caacgcggga aaaccgttcg
     4081 caccggccgc gaatcgcgca aaaaccgcta gcatctcccg gttgcccagg gccatgcccc
     4141 ccaccttggg gattgtgctt cccgccgccc gccgccgttg ccgagccgac tccgatggac
     4201 caccgatttg ctgcttcgcg ctccgcttcc ctggccgtgc tcgccgcctt cgccggtgca
     4261 gcgatgcttt tgctgctcgg cgcctgtgag cgcgaggccc cgcagacccg cagtgccgcg
     4321 ctgacccaga acgccccgcc gccggtgccg cgaccttcgg aaaccggcgg gccgcaggtg
     4381 acgctggtca tgaagacgct gaccaatccg ttcttcgtcg acatggagcg cggcgcacga
     4441 cgggccgcga ccgagaccgg agcacggctg ctggtgcgcg cggcggcgca ggagacctcg
     4501 atcgaccagc agatcgagat cgtcgagacc gcgatccgcg agcgcaccga cgccatcgtc
     4561 atcgcccccg gcgattcggc ggcgctgatc ccggtgctgc gcaaggcccg cgatgccggc
     4621 atccggctgg tcgtcatcga caatccgctc gatccggtgc tcgccgaacg ggcgggcctc
     4681 ggcgacgtgc cgctggtcac ggtcgacaac gaacgcggtg ccgccctcgc cgccgatcag
     4741 gccgccgcct cgctgcaggg gccggtgccg acgctgctgc tcgaaggcat ccgcggggcc
     4801 gccaacgccg aggcccgccg gcgcggggcg ctctcggcgc tggcgagaca cccggccgtg
     4861 caggtcgtcg ccagcgagag cgcgcactgg aagatcgacg aggccttcga ggtcgcgcag
     4921 cgcgagttca gcgagcaccc cgagctgcgc ctggtcgtct gcgccaacga catgatggcg
     4981 ctcggcgtga tccgctacct gaaggacgcc ggccggccgg acgtgcgcgt gaccggctac
     5041 gacgcgctgc ccgaggcgcg cgaggcgctg gcgcagggct ggctgtacgc gaccgtcgac
     5101 cagcgggcgg ccgaacaggg cgagaccggc gtgcgctacg cgctgcgggc gctcgccggc
     5161 gaggtgctgc cggcgcgcac gctggtgccg gtgacgctgg tcctgcgccc tgacggcgga
     5221 ggcgcggacg ggcgcaccgg tgaacgctga tccgcgccgc tggctgcgcc gcgcgacgct
     5281 cgcctggcgc atcggtctgt acctcgcggc cgcgagcgcg gtgccgctgc tgctgaccgg
     5341 ctacctgtcc tatcacatgg ccgccgatgc gctgctgcgc gaggccacgc attcggttgc
     5401 cgccgcggtc gaggcgcagc gccgttacat cgaggcgcag atggcgcagg tcgagagcct
     5461 gctcgccaac ctcgccggcg tcgaggcgat caccgccgcc gcgatcgagg aacagtccga
     5521 cgccgacacc tatgaccggc tcgcgaccca ggcgcgcatc ggctacatcc tgaacggcta
     5581 ttccagcctg cgcggcctgg tgtcgatcga cctgatcggc gccggcggcc ggcattacca
     5641 cgtcggcgac acgctcgaag ccgtgcgcct cgacgaggag cagcgccggc ggctgttcga
     5701 ggccgcccag cgctccgggc gcaccgtgca ctggagcggc atcggcccga acatcaacgt
     5761 gcattcggcc taccgcaagg tgatcacggc cgcgcgcctg ctgtatcggg tcgagcgcga
     5821 gacgctgcgc cgcgagccgg tggcgctgct gctggtcagc tacgaccccg acgagctggc
     5881 tcgccagttc gacgacgtcg cgctcggcaa cggcgcctgg ttcgtcgccg tcgatggcga
     5941 cggccggctg ctgtaccacc ccgatgcccg gctgcgcggc gagcgcctgg ccacgcccct
     6001 gctcgaaacc ctgcgcgatg cgcagggcag ccgcagcgtg aacatcgccg gacacgacta
     6061 cctgctcgcg catgcccgcg ccgtcacgct cggctggacc atcgccggct tcgtgccgct
     6121 cgacacgctg gcagcgccgg tcgcaccgat ccgctgggcg agcttcgggc tggtcgccgg
     6181 ctgcctgctg ttcgcgctgc tgatggcgat ccggctgcac gccaaggtca tcgcgccgct
     6241 gcgcgcggtg atccgccagc tgcagcgctt cgagcgcgac ccggccggcg ccgagcacct
     6301 cggcatcacc aatgacgacg agatcggcga gctgacgcgc tggtacaacc gctcgctcga
     6361 cgccgtgcac gcgctgcgcg acagcgagga gcgcgcgcag cacgcggcgc tgcacgacgt
     6421 gctgaccgga ctcgccaacc gcacgctgct catcgatcac atcacgcacg cgcaggcggc
     6481 cgtgcgccgc cagcccgagc gcccgtgcgc gctgctgttc atcgacctcg atcacttcaa
     6541 gccggtcaac gaccgcctcg gtcacgaggc cggcgacaag ctgctctgcg cgatcgcgca
     6601 gcgcatcggc gactgcgtgc gcagcatcga cgtgctcgcg cgcctcggcg gcgacgagtt
     6661 cgccgtgctg ctgacgcgcc tcgatgaggc cggtcgggcg ctgcgcatcg ccacgcgcat
     6721 cgtcgaatgc gtgcgccggc cggtgacgat cgacggccac gaggtgcagg tcggtgccag
     6781 catcggcatc gccatcggcg acggcaccca ggatgccgag gcgctgctgc gcgatgccga
     6841 catcgcgatg taccgcgcca agcagaccgg caagtcgcgc tgcgagctgt tcgatgccgc
     6901 gctcggcgcg caggtgctgg agcgcgtcgg tctggagttc gagctgcgcc gcgcgctgga
     6961 gcgcggccag ctggcgatgc actaccagcc gatcgtcgcg ctcgccgatg accgcatcgt
     7021 cgcctgtgag gcgctgatgc gctggcagca tccggagctt ggcgcggtgg cgccggatcg
     7081 cttcatcccg atcgccgagg aaaccggcct gatcgccgaa ctcggggcct gggcgctcgc
     7141 cagtgcctgc gcgcaggcgg cgagctggcg ggcgcgctgg ggcgaggcgg cgccggcgat
     7201 cgccgtgaac ctgtcgccgc gccagctcgg ccggccggat ttctgcgaac aggtcgctgc
     7261 ctgcttcgcc gagcacggca tcggccccgg catgatccat ctggagatca ccgagaccgc
     7321 cctgctcgcc gacctcggcc acgccgaccg cctgctcgcc tgcctgcgcg gcctcggcgc
     7381 gaccatccac ctcgacgact tcggcaccgg ctattcgtcg ctgagctacc tgagccggct
     7441 gcaggtcgac gcgatcaaga tcgaccgcgc cttcgtgcgc cgcctcgatc ccgcgcacga
     7501 cggaccggat ctggtgcgcg cgatcctgct gatcgccgac gagctggggc tgaccgtggt
     7561 cgccgaaggc atcgagaccg agtaccagca ccagcgcctc gccgcgctcg gctgcgcgca
     7621 cggtcagggc tggcgcttcg gacgggcggc ggaaccggcg gcgatcgaga cgcagctgcg
     7681 ccggcagctg gtcaggaccg gctgacgcac cgcccggcgc ccggatcagc gccggcgcga
     7741 gcccggagtc ctcgtcaccg ggccggcgtc ttcaggcggc cgtggctgcg gcagcggcgt
     7801 gtcgccggcg agttcgtcga ggatcgggca gtccgggcgc gcgtcgccgc agcaggcagc
     7861 ggcgaggcgc gccagcgcgt cgcgcatcgc gctcagcgac gcgatgcgcg cgtcgagttc
     7921 ggcgatgtgg ccggcggcga ggcgcttgac ctcggcgctg gcgcgcgagc ggtcctgcca
     7981 cagtgcgagc agccgggcga tgtccgccac cgggaagccg agatcgcgcg cgcggcgcac
     8041 gaagcgcagc acatgcacat ccccggccgt gtaggtgcgg tagcccgccg ccgtgcgcgc
     8101 ggccggggcg agcaggccga gcttctcgta gtggcggatc atgcgttcgg agacgcccga
     8161 ggcgcgggcg gcggcaccga tgttcatcgc ggacggctcc cggcgggcgg cgtcaggccg
     8221 ctgccggacg cgcttcgccg agcaggtggg cgaaggcgcc gatcagcgcc gattcgacct
     8281 cggccggcgg actcgcgtac agcgcgctcc aggcctcggc gcggaaggct tcgtagtggc
     8341 cggcgtgatc gggatgctgc tgcagccagt gcaggcgcac ggcgtgaccg gtcagcacgt
     8401 cgatgacgat gtggccggcg acgtgcaggc cggggttggc gctgcagagg tgcgcgagcg
     8461 ccgcgagcgc ctcgatgttg agctggcggt actcgggcga ctggatcttg tcgagcaggc
     8521 ggttgacggt cagcgcgaag ttggtctcgg ccgcggtcat gtcggcgcgc aaccgcgcgc
     8581 tgtcgaggca gttgccgatg tcgaggcgct caccgagcac cagcccctcg cagtgcgcga
     8641 gcacggccca gacctgctcg tagaagtgcg ccgacagctg cgtcagcacg ccgtggcgcc
     8701 ggcgccaggc cagccagtcg acgacggcct cggctccggc gtcctcctgc gggtcgggca
     8761 cggccaggcc gtgcgcggcg tcgtcgcggc gcagcgattc gagccgcgcc agccggctga
     8821 ccggattgtc gccgcgcacc acggcctgca cgcgccccat gaacgcccac ggtccgagcg
     8881 tctgcacgcg ccggaaggcc tcgggctgcg acagccggtg ctcccaggcg taatcgccga
     8941 cgacgagcag caccagcggc caggcgcgga tcgtcatcgt gtcgcggaac accgccgggt
     9001 cggtcttcag cagcgtctcg accgcgagca cgacctcctg caccagcatg cgtccggccg
     9061 gatcgtcgcc ggcgtactgg cgcaggcagg cgacgatctc gcggttgccg agcgcggtgc
     9121 tgaccaccga gccatcgctg tagccgcgcc cgagcgcgat ctgcttgcgg tgcgcgacga
     9181 tgttggcgac cgcgtcctca agcttcggat cgtgcttgtc gagcagcccg gcgcagcggc
     9241 gcagcagccc ccagtggccg gcctcggcgg cgagcgcgta cgcggcctcc agacaggcgc
     9301 gcacgctgcg cccgtccggc agcggcgcgt cgatgccgtc gcgccgggcg gcgatctcca
     9361 gcagcgcgat gacctcgtgc aggttgcgcg tcgcggcaag gcgcaccggc accgccggcg
     9421 gcggcaggtc ttcgagccgc tgcaggtcgg cggcgtcgag ttcgcgggcg gcggccgggt
     9481 cgatgtcgag caggttgccg gcgctgcgct cctgttcgag cggcgcgaac tcgaagtggt
     9541 agtcgcgcag gcggtcgaag cgcttgcgcc cggcggtggc gatcagctcc gacagcgggc
     9601 gcacgcgcac cggcaggccc tcgcagctgc cggcgcagag cgcgcgcagg aagtcgagca
     9661 gctccccggc gccgtcggtg gcgagcatgc gcgcggtcag gcgcagcgcg aacaccggct
     9721 ggccggcctg gtcccagtgc cggcgcacgt gcgcgaactc ggccttgcag cggctgatca
     9781 gcacgcggtt gctgaggttc aggtagaagt cctggcgttc gaggaactgc ggcaggaaca
     9841 cgaccgtctc gccgccgatc gtgaacacct ggcaggtgac cagcgtgccg acgccgcgcc
     9901 gcggccggcc ggagagaccg agcgccgcgt tgcgcccgac gttggtcatg gcctcgacca
     9961 gctcgctcgc ctgacgcacg cgcaccggca gcacggcctc gggcgtctgc gcgcgcacgc
    10021 cggcatcgag cagcgcggtc tcgaccgccg ccgtgtcggc gatcagcgcc acctgcaccg
    10081 tgcattcgcg ccgccggccg atgcggtggc ggcggttcag cgggtcgatg tcgccgggat
    10141 gcagccagcc ctcgtcgatc agcagcgcca gccagtacag gctctgcgcc cagtacagcg
    10201 gcacgttggc gttcggcagc cgcggctgcg agccgggccg cgccttctcg gcggcgacgg
    10261 atttttccgg caccaggtac agctccggca gcagcggtcc ggcgccgcga tcgaccagca
    10321 gcggcgccag gcgctcgcgg tattcgtgcg cctgaccgtc gcgcccgcgc atcaggccgt
    10381 cgagcatcag gtaggtgaag aacagcggcc attcggactc gatgcgctcg aagcgcgcca
    10441 gctcgccgct ctcgtagtgc aggcgcgtgt ggtcctccag cacggtctgg tgcccgtcgc
    10501 gcaggaagcg cttgcagccg tagcggccct gcagcttggc gacgacctcg tggcgggtgc
    10561 gctcgaccag ctcggcgtcc tcgaccgccc aggccggaaa gccgatgacg ctcagcagcg
    10621 ccgcatcgac ctccttcgag cgcgactcgc gcggcagcgt ggcttcgagc aggttgcgca
    10681 tgcgcgcgat gtcgtcggag gacacgtgga tcaccgaggc cggaccgccc tcggcgccga
    10741 acaggtcgaa gccgcgcagc gcctccagcg cggccttggc catgccgacc gagctggcgt
    10801 tgatctccgg gtcgccggtg ttgatcttgt cgccgcgctc ccagatgccg tagtccggcg
    10861 tgcggtaggc gcgcgcgatg tagtgcgcga gcgcctgcac gaaatcgacc tcgtcgagcg
    10921 aatacacgac gcgcaggccc gaggcgatca tctgcaccag catcagcagg aacaccgagg
    10981 tcgcgtcgat ctgcagatgg ccccagccgt catcggcgac caccggctcg ccggtgcggc
    11041 tgccgtactt cgcgtgcagc gcgtcgatcg gcgccagcgt gtgcttgaag cgctcgacct
    11101 tgtgcgcctg cttcatcatc gcggtcagca ggccgcgcat cgtcttcacc gcgccctgct
    11161 cgaagcggta ggcgcgcgga tcgccgccgc ggtggcggcg cagcgccagg ccgcagcccc
    11221 acacgcacag gatcgaatag acgttgtcgc gcacccaggc gtcggtgtag tcgccgtgca
    11281 cggtgacggc ggtgctcgcc ggcagcaggc cggtgaccgg gtcctggcgc gcgagcatca
    11341 cggtttcgat ctgcgcgcag agttcgccga ggcggacggc gtcgggggtg cgggcgctgg
    11401 gattcaagcg tggactccgg ggtcggcgac gggaacgcgg gccggcggcc gctggtcgtt
    11461 cagcatgccg gggggcgcgc acgaggccaa gcctgcctgc aggcattgac ggccgtcatc
    11521 gcgcccgcgc ccgcgctctg cgaaatgcat tttgtcatat gcaatcgcat cccggatgcg
    11581 acctgtcaga ctgcgtttgc gcagcgcacc acgccctttc catgccccgg ccccttcccc
    11641 gccgcagagg atttgcccga tgagtgttgc gccgtccccg gacatcgtgc aggccatcgt
    11701 caccggcctg tggcccgatc cgtacccgaa ggccgatccg ttcgcggtgc tcggcatgca
    11761 cgtcgaggcc ggggccggag tcgtggtccg cagcttccag cccgacgcgg aatcggtcga
    11821 ggtgctcgat gccgcgagcg gccggcgcgt cgcgacgctc gaacgcatcg acgccgccgg
    11881 cctgttcgca ggctccgtgg cgcgccggaa gaaccccttc gcgtaccggc tgcgcgtgcg
    11941 ctacgccggc accgtcgtcg aactcgacga cccctaccgc cacgccagca gcctcggcga
    12001 actcgacctg cacctgctcg gcgagggccg gcactggcgg gcgttcgagg cgctcggcgc
    12061 gcactgcctg aggctcgccg gcagcgacgg cgtgcgcttc gcggtctggg caccgaatgc
    12121 gcggcgcgtg tcggtggtcg gcgacttcaa ccgctgggac ggccgccggc acccgatgcg
    12181 cttccacccg ggcagcggct gctgggagct gttcatcccc ggcctcgccg aaggagtgct
    12241 gtacaagtac gagctgctcg gcccggacgg cacgccgctg ccgctgaagg ccgatccctg
    12301 cggctacgcc gccgaactgc ggcccgatac cgcctcgcgc gtcgcccgcc tcgatcacca
    12361 cgcctggaac gatggcgact ggatggcgac gcgggccgcg cgcatcgccc gcgacgcgcc
    12421 ggtcagcatc tacgagctgc acctgggctc gtggcggcgc agtcccgacg acccggaccg
    12481 gccgctcggc taccgcgagc tcgccgatgc gctggcgccc tacctgaatg atctcggctt
    12541 cacgcacgtc gaactgctgc ccgtcaccga gcatccgttc gacggctcct ggggctacca
    12601 gccgctcggc ctgtacgcgc cgacctgccg ccacggcagc ccggcggact tccagtattt
    12661 cgtcgaccgc ctgcatcagg ccggcatcgg cgtgctgctc gactgggtgc cggcgcactt
    12721 cccggccgat gcgcacggcc tcgcgcgctt cgacggcacg gcgctctacg agcacgagga
    12781 cccgcgcctg ggctttcatc cggactggaa cacgctgatc tacaacctcg gccgtcacga
    12841 ggtcgcgaac ttcctgatcg ccaacgcgct gttctggttg gagcgctacc acctcgacgg
    12901 cctgcgcgtc gatgccgtcg cctcgatgct gtacctcgac tactcgcgc
//
LOCUS       DAOQIE010000070        12911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2370_length_12911_cov_7.401914, whole genome shotgun sequence.
ACCESSION   DAOQIE010000070 DAOQIE010000000
VERSION     DAOQIE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_12911_cov_7.401914"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PK144_06210"
     CDS             <1..1487
                     /locus_tag="PK144_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005891402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF65357.1"
                     /translation="VGPPYLYVDAPGLDAGALDALAQALIAVAGVETITPIDVLPGLR
                     RQLQLDALLGAMADPVFGLDEHGAVLLANPAAAGVAGRPEAALAGVGLDALFGEPGLQ
                     AQLRDNGFRLPIREVQLEGAAFLAQVEPLAEADGRVAGGVLTLHAPSRIGERLHALQN
                     PDEGGFERLLGDSPPMRVLKARAARVAAVEAPLLILGETGTGKELIAHACHHASRRRG
                     APFLALNCAALPESLAESELFGYAPGAFSGAQRGGKPGLLELADRGTVFLDEIGEMSP
                     YLQAKLLRFLNDGSFRRVGGSREQRVDVRIVSATHRDLERMVAEGRFREDLWYRLNVL
                     TLTVPPLRERGDDIRLLAGHFTVRACAQTGRPPCRIGTSAAALLRQHLWPGNVRQLEN
                     VVFRAVTMSDRSVLEAADFELADNAIHAGEEGPPSAPAVPVAAFSGDPAGDGWFAAVE
                     RCERELLARLWPQFPSSRRLAAHLGTSHTMIAKKLRKYRIGRES"
     gene            1872..2249
                     /locus_tag="PK144_06215"
     CDS             1872..2249
                     /locus_tag="PK144_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF65358.1"
                     /translation="MQIVSTPAAPAAIGPYAQGVIAAGLLYTSGQIPLDADGRFVAGD
                     IEVQTTQVLANLRAVIEAAGAGFADVIRTTVYVADLDDFPRLNAVYARHFGEHRPARS
                     TVQVARLPRDARVEIEAIVRLPG"
     gene            2332..3720
                     /locus_tag="PK144_06220"
     CDS             2332..3720
                     /locus_tag="PK144_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF65359.1"
                     /translation="MAATLHRGLEQRHITLMSLGAAIGVGLFLGSGAAIKLAGPAILI
                     AYAIGGLAMFFIMRALGEMAINQPVAGSFGRYAHEYLGPLAGYLTGWNYWFLWIVTAM
                     AEITAVGIYMQMWFPGSPAWLWALAAFVSMTAVNLIAVKAYGEFEFWFAAIKVVAIVA
                     MILIGGGVIFFGLGNGGEAVGISNLWAHGGFLPNGVTGVLMSLQMVMFAYLGIEMIGL
                     TAGEVKDPKKALSRAIDSVFWRILIFYVGALFVVMALYPWNEIGTQGSPFVLTFQKVG
                     IPGAAGIINFVVLTAALSSCNSGIYSTGRMLFNLAEQGQALPVFSRVSRGGVPVWAIL
                     GSSAMLLIGVLLNYLVPEKVFVWVTSISTFGAVWTWAMILCSQLRFRTLKGAPAEYRM
                     PWWPYSAWFSLGFLALVVGLLAWFPDTRIALYVGPLWLVLLVVLYHVLGHHRSAPQSG
                     SLPAALAADTAS"
     gene            3978..4883
                     /gene="yedA"
                     /locus_tag="PK144_06225"
     CDS             3978..4883
                     /gene="yedA"
                     /locus_tag="PK144_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HNF65360.1"
                     /translation="MTDSTLALPAAAPLRLAPAIVLSLAAVYVIWGSTYLAMRLVLVD
                     IPPFLQIGSRGMLAAVLLFAWLRWRGVPWPDRRGWRDAGIVGVLLLGGGTGGVAVAQQ
                     WVGSGLAAVVVATVPLWAALINGCWGQWPRRAEWIGIGIGFLGVALLALEKDFRAEPR
                     GAFVLLAASALWAYGSMLSRRLRLPPGASGFAAEMLVGGGVLVLAGLVTGERIGWPLH
                     PATPWLWGYLVVFGSIVGFSAYMYLVSRVRPALATSYAYVNPVIAVLLGVSFGGESIS
                     AGGYVAMALVLAAVVLITRAGERQA"
     gene            complement(4939..6345)
                     /locus_tag="PK144_06230"
     CDS             complement(4939..6345)
                     /locus_tag="PK144_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF65361.1"
                     /translation="MYNANALPPDLLDELRALLGDRLSTGLAVREHHGRDESSHPAAL
                     PDAVVWPETTEEVSTIARLCHAFRVPMIPFGVGSSLEGHVIPLHGGVSIDLSHMNRIL
                     ALHVEDLDATVQAGVTRKQLNAWLHDSGLFFPIDPGADATLGGMTATRASGTNAVRYG
                     SMREAVLALTVVLADGRVIHTGTRARKSAAGYDLTRLFVGSEGTLGIVTEITVRLHPH
                     LEAISAAVCGFPDVASAARTVIQTIQTGVPVARAELLDALSMRAINAHSKTNWPEVPT
                     LFFEFHGTEAGVAEQAETVQALAHEHGGTDFRWATRQEERTQLWAARHDAYFACLQLR
                     PGSRSLTTDVCVPISRLAECIEATAADVAGMPMPVPLLGHVGDGNFHLLVLTDPDSAA
                     EREAAHALSHRLVLRALAMGGTCTGEHGVGFGKMGYLAEEHGEALDVMRAIKHALDPY
                     GLMNPGKIVETEPQDARR"
     gene            6656..7390
                     /locus_tag="PK144_06235"
     CDS             6656..7390
                     /locus_tag="PK144_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF65362.1"
                     /translation="MPELEGIGGWLFAVLQIIAIDILLGGDNAVVIALACRRLPEDKR
                     RLGVRWGVAGAIVLRIVMIAFALQLLLVPGLKLIGAALLLWIGIKLLQPEADDGHDGI
                     EGGTTLLAAVRTVIVADAVMSIDNVVAVAAAARGELALVVFGILVSVPIIVWGSHFVL
                     RLMDRFPVIIVIGGALLGWIAGGLAVHDPLLERLGFDAPDWAHWPASLVGALAVVLIG
                     RQLAARAGRAAPAPLEDLLSEARRDE"
     gene            7435..7863
                     /locus_tag="PK144_06240"
     CDS             7435..7863
                     /locus_tag="PK144_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF65363.1"
                     /translation="MSVSKILVPVDGTPESADAVAHALAIVAERGGELHLLHVRLPVE
                     SCHAKLLVDGRTLHDWHREEGEQALAPSLAQCRAAGVEPLCHVIVGHLAETIVRFAHE
                     QHFGLVVMRRHPQAALTHLLLGSVCEDVQAQCRIPVTVLD"
     gene            7866..8315
                     /locus_tag="PK144_06245"
     CDS             7866..8315
                     /locus_tag="PK144_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HNF65364.1"
                     /translation="MASRRRLLAWLAALPAAWLAARAAAREGRPLTAFAATSLEDALR
                     AQYGDRPRLRDAALRIEAPQSADNAAQVPLRIDARSRPVLALTLFAEHNPVPLLGRWR
                     FGEGVEAWLALRIKLAASGRVCAVAELADGALLYAETRVEVGTGACG"
     gene            8321..8656
                     /locus_tag="PK144_06250"
     CDS             8321..8656
                     /locus_tag="PK144_06250"
                     /inference="COORDINATES: protein motif:HMM:NF020350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HNF65365.1"
                     /translation="MSPDPVRIEARFLDGRTEVRLLIRHPMHTGRERDPDSGAPIPAL
                     YIDTLIVERIAADGRTHELLQADFGPGIARNPFHHFAFGGGAPGETVRVRWRDTGGNE
                     ASGETVITA"
     gene            complement(8792..9910)
                     /gene="ald"
                     /locus_tag="PK144_06255"
     CDS             complement(8792..9910)
                     /gene="ald"
                     /locus_tag="PK144_06255"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNF65366.1"
                     /translation="MQIGVPKEIKNHEYRVGLTPASVRELVAHGHTVFVEHDAGLGIG
                     CDDAQYLAAGATVLGTAAEVFERAAMIVKVKEPQPVECRRLRAGQLLFTYLHLAPDPE
                     QTRLLVESGVTAIAYETVTGARGGLPLLAPMSEVAGRMAIQAGAHALEKAHGGRGVLL
                     GGVPGVAPAQVTVIGGGVVGAGAIRMAVGMGAHVTVLDRDIDRLRALDIEYGPRLTTL
                     FATRDAIEAQVLESDLVVGAVLVPGAAAPKLVTRAQLAQMRPGAVLVDVSIDQGGCFE
                     TSHPTTHAEPTYVVDGIVHYCVANMPGAVPRTSTFALNNATLPYTLALADKGFPQALF
                     DDAGLMAGLNVHAGRITCRAVAEALDYECVEPGEWLRG"
     gene            10035..10256
                     /locus_tag="PK144_06260"
     CDS             10035..10256
                     /locus_tag="PK144_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65367.1"
                     /translation="MASAHGRVVAAVRKLSGKQGRFPAWQGTIDARHLHFDARQGMPP
                     ARQARIVDPHGFAAAIGSGGPRRSAGGRG"
     gene            10369..11706
                     /locus_tag="PK144_06265"
     CDS             10369..11706
                     /locus_tag="PK144_06265"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNF65368.1"
                     /translation="MPTVPAKSRSPALPPRLLAAIRAEQQASERLIGQVQLAVVLTFG
                     LLWAIAPSPPSPAPSPVPWALAGYLLVTLTRLAWTQRRRLPDWAAYLSIVADFALLYG
                     LIWSFHVQYMQPPAFSLKAPTLLYAFIFVVLRVLRVELRFVIAAGAVAVAGWAALVGL
                     AIGGNLHDAAITRDYVHYLTANAVLLGGEFDKLATMLTVTLILALAVARGQRLLVRAT
                     VESNAAMQLARFLPMAVAVRIAAATERPPTGDGELAPATILFLDLEGFTTLAEALPPP
                     QLIALLNEVFEAVVAPIERHGGVVDQFIGDAVMATFNLPQPHADHAAAAVAAALDIQT
                     LMATHRFGTGVRLAARIGINTGIVVGGLVGAGERLAYTVYGDAVNLAARLEPLNKDYG
                     TRVLLSEATRLAAGEARFAWRAVGEVRVRGRSVATPLYTVDAPEAAAPADDAG"
     gene            11726..12394
                     /locus_tag="PK144_06270"
     CDS             11726..12394
                     /locus_tag="PK144_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65369.1"
                     /translation="MSSSGLHDRFCRLWHAHGGRDAGQVFAGLAAHYAEPQRHYHTLA
                     HIRRCLRTLDHYRASVPQPDLVELALWCHDVIYAPAARDNEARSADWWRAQAAGFAPA
                     SVDLVADAILATTHRQPPTGAVAAWTVDIDLAGLALSRSSFARDGRKLRAERPELGEA
                     EYGAGERAFLSGLLARARIYYTEALYADGEARARENLRRRIATLTGARHAGAAGGLSA
                     LGTG"
     gene            12351..>12911
                     /locus_tag="PK144_06275"
     CDS             12351..>12911
                     /locus_tag="PK144_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNF65370.1"
                     /translation="MPERPAGFQPLGRADEGSPSLAQSAAALFDSFGRLLASAGSLPT
                     YGYSGREPDATGLVYYRARYYAPELGRFTQPDPVGLAGGINRYAYVGGNPIDFRDPSG
                     FVVAPPVSPGNALPAYAGAVGGVAVTAAETGAAGLEGSGFLTGAASGVRTLLTYAGRG
                     LDPMAEATECRGSISVDTRIGEIPPPR"
BASE COUNT         1691 a         4555 c         4640 g         2025 t
ORIGIN      
        1 aggtcgggcc gccctacctg tacgtcgatg cgcccgggct cgatgccggc gcgctcgacg
       61 cgctggcgca ggcgctgatc gccgtcgccg gcgtcgagac catcacgccg atcgacgtgc
      121 tgcccggcct gcgccggcag ttgcagctcg atgcgctgct cggcgcgatg gccgatccgg
      181 tgttcgggct cgacgagcac ggcgcggtgc tgctcgccaa cccggccgcc gccggcgtcg
      241 ccggccggcc ggaagcggcg ctcgccggcg tcggactcga tgcgctgttc ggcgaacccg
      301 gcctgcaggc gcaactgcgc gacaacggct tccggctgcc gatccgcgag gtccagctcg
      361 aaggcgcggc cttcctcgcg caggtcgaac cgctggccga ggccgacggc cgcgttgccg
      421 gcggcgtgct gacgctgcac gcgccgagcc gcatcggcga acgcctgcac gcgctgcaga
      481 accccgacga aggcggcttc gagcgcctgc tcggcgactc gccgccgatg cgcgtgctga
      541 aggcccgggc cgcgcgcgtc gcagcggtcg aggcgccgct gctgatcctc ggcgagaccg
      601 gcaccggcaa ggaactcatc gcccacgcct gccatcacgc cagccgccgg cgcggcgcgc
      661 cgttcctggc gctcaactgc gcggcgctgc ccgagagcct cgccgagagc gagctgttcg
      721 gctacgcgcc cggtgccttc agcggcgcgc agcgcggcgg caagcccggc ctgctcgaac
      781 tcgccgaccg cggcacggtg ttcctcgacg agatcggcga gatgtcgccg tacctgcagg
      841 ccaagctgct gcgcttcctg aacgacggca gcttccggcg cgtcggcggc agccgcgagc
      901 agcgcgtcga cgtgcgcatc gtcagcgcca cgcaccgcga cctcgaacgc atggtcgcgg
      961 aagggcgctt tcgcgaggac ctctggtacc ggctgaacgt gctcacgctg accgtgccgc
     1021 cgctgcgcga gcgtggcgac gacatccgcc tgctggccgg gcatttcacg gtgcgcgcct
     1081 gcgcccagac cgggcgcccg ccgtgccgga tcggcacgag tgccgccgcg ctgctcaggc
     1141 agcacctctg gcccggcaac gtccgccagc tcgagaacgt cgtgttccgg gccgtgacga
     1201 tgagtgatcg cagcgtgctc gaagccgccg acttcgagct cgccgacaac gcgatccacg
     1261 ccggcgagga agggccgccg tccgcaccgg cggtgccggt ggcggctttt tccggcgatc
     1321 cggcgggcga tggctggttc gcggccgtcg agcgctgcga gcgcgaactg ctggcccggc
     1381 tgtggccgca gttcccgtcg agccgccggc tcgccgcgca tctgggcacc tcgcacacga
     1441 tgatcgcgaa gaagctgcgc aagtaccgga tcggtcggga gtcctgaagg cgggcgttcc
     1501 ggatggccgg acggaaggcc ggaagcttgg tttgatttat ttttatacca tcatattttt
     1561 tatatcaccc tgactgaaaa gcgggtgagc agcagacccg acgcgtctgt agccggacgc
     1621 atacgcgtag ccaatcgctt acagaagctt ccctgccgat caagtcattg atccggatca
     1681 acttgatgcc ggttctgccg gtggccggcc gtctgctgta ttcgatcgtg tacggaaggt
     1741 ccggcgccgg atcgagcaaa aatccttcaa gctgctgaaa tacaagcgtt tctgatgcct
     1801 ggcatcggga ttgccaagga tggttacgcc tcccgtccgg gggccgtccc gatccacgga
     1861 gcccgaatcc catgcagatc gtttccaccc ccgccgcgcc ggccgcgatc ggcccctacg
     1921 cgcagggcgt catcgccgcc ggcctgctgt acacgtccgg ccagattccg ctcgatgccg
     1981 acggccgctt cgtcgccggc gacatcgagg tccagaccac gcaggtgctc gcgaacctgc
     2041 gcgccgtgat cgaggccgcc ggcgcgggct tcgccgacgt gatccgcacc accgtctatg
     2101 tcgccgacct cgacgacttc ccgcgcctga acgccgtcta tgcccggcac ttcggcgagc
     2161 accggccggc gcgctcgacc gtgcaggtcg cgcgcctgcc gcgcgatgcg cgcgtcgaga
     2221 tcgaagccat cgtgcggctg ccgggctgat ccccgcgcgc cgcctttccg cacccggcac
     2281 ccacagaccg gggcggttct tcctgcaggc accacctagc ggagcaacac catggcagcc
     2341 accctgcatc gtggtctcga acaacgacac atcacgctga tgtccctcgg cgccgccatc
     2401 ggcgtcgggc tgttcctcgg ctcgggcgcg gcgatcaagc ttgccggccc ggccatcctg
     2461 atcgcctatg cgatcggcgg cctcgcgatg ttcttcatca tgcgtgcgct cggcgagatg
     2521 gcgatcaatc agccggtcgc cggctccttc ggccgctacg cgcatgaata cctcggcccg
     2581 ctggccggct acctgaccgg ctggaactac tggttcctgt ggatcgtcac ggcgatggcc
     2641 gagatcaccg ccgtcggcat ctacatgcag atgtggttcc ccggctcgcc ggcctggctg
     2701 tgggcgctcg cagccttcgt gtcgatgacg gcggtgaacc tgatcgccgt gaaggcctac
     2761 ggcgagttcg agttctggtt cgcggcgatc aaggtcgtcg cgatcgtcgc gatgatcctg
     2821 atcggcggcg gcgtgatctt cttcggcctc ggcaacggcg gcgaggcggt cggcatctcc
     2881 aacctgtggg cacacggcgg cttcctgccg aacggcgtga ccggcgtgct gatgtcgctg
     2941 cagatggtga tgttcgcgta tctgggcatc gagatgatcg gcctgaccgc cggcgaggtg
     3001 aaggacccga agaaggcgct gtcgcgcgcg atcgactcgg tgttctggcg catcctgatc
     3061 ttctacgtcg gcgcgctgtt cgtggtcatg gcgctgtatc cgtggaacga gatcggcacg
     3121 cagggcagcc cgttcgtgct gaccttccag aaggtcggca ttcccggcgc ggccggcatc
     3181 atcaacttcg tcgtgctgac cgccgcgctg tcctcgtgca acagcggcat ctacagcacc
     3241 gggcgcatgc tgttcaatct ggccgagcag ggccaggcgc tgccggtgtt cagccgggtg
     3301 tcgcgcggtg gcgtgccggt ctgggcgatc ctcggctcca gcgcgatgct gctgatcggc
     3361 gtgctgctga actacctcgt gcccgagaag gtgttcgtct gggtcaccag catctcgacc
     3421 ttcggcgcgg tgtggacctg ggcgatgatc ctgtgctcgc agctgcgctt ccgcaccctc
     3481 aagggcgcgc cggccgaata ccgcatgccg tggtggccgt acagcgcctg gttcagcctc
     3541 ggcttcctgg cgctggtcgt cggcctgctg gcctggttcc cggatacccg catcgcgctc
     3601 tacgtcggtc cgctctggct ggtgctgctg gtggtgctgt accacgtgct cggccatcac
     3661 cgcagtgccc cgcagtccgg gtcgttgccg gctgcgctgg cggcggatac ggcgagctga
     3721 tcgcacggca gcgcaggctg gtggcggggc ggcgcaaggc cgccccgccg tctttgctgc
     3781 aagcccgcga tgtctgcaga aggccggctg ccggcgtacg ggcgccgcca ccgcggcagg
     3841 ccggcacgaa gccggacgca gctccgggcc gcttcgccac cccgatcgcc gttctccggc
     3901 catgcgccgc gcccatcgcc agccttcgca cggcgggcgg atgatgccgg cccctgcgca
     3961 ccccgccctt caccgtcatg accgattcca cgctcgccct tcccgcagcc gcgccgctgc
     4021 gcctcgcccc ggccatcgtg ctgtcgctcg ccgccgtcta cgtgatctgg ggctcgacct
     4081 atctggcgat gcgcctggtg ctcgtcgaca tcccgccgtt cctgcagatc ggcagccgcg
     4141 gcatgctcgc cgcggtcctg ctgttcgcgt ggctgcgctg gcgcggcgtg ccgtggccgg
     4201 accggcgcgg ctggcgcgat gccggcatcg tcggcgtgct gctgctcggc ggcggcaccg
     4261 gcggcgtcgc ggtcgcgcag cagtgggtcg gctccggact cgccgcggtc gtggtcgcga
     4321 ccgtgccgct gtgggcggcc ctgatcaacg gctgctgggg gcagtggccg cggcgcgccg
     4381 aatggatcgg catcggcatc ggcttcctcg gcgtggcgct gctcgccctc gaaaaggact
     4441 tccgcgccga accgcgcggg gcgttcgtgc tgctggcggc ctccgccctg tgggcctacg
     4501 gctcgatgct gtcacgtcgc ctgaggctgc cgcccggtgc gagcggcttt gcggccgaga
     4561 tgctggtcgg cggcggcgtg ctcgtgctcg ccggcctcgt gaccggcgag cgcatcggct
     4621 ggccgctgca tccggcgacg ccgtggctct ggggctatct ggtcgtgttc ggctcgatcg
     4681 tcggcttcag cgcctacatg tacctggtca gccgcgtgcg tccggcgctc gcgaccagct
     4741 acgcctacgt gaacccggtg atcgcggtgc tgctcggcgt cagcttcggc ggcgaatcga
     4801 tcagcgccgg cggttacgtg gcgatggcgc tggtgctggc cgcggtcgtg ctgatcacgc
     4861 gggccggcga gcggcaggca tgaatgccgc ggccgggatg gggcggggcg gccggaggct
     4921 tcgcaagccg ccgcgccttc agcgccgtgc gtcctgcggc tcggtctcga cgatcttgcc
     4981 cggattcatc agcccgtagg gatcgagcgc gtgcttgatc gcgcgcatca cgtcgagcgc
     5041 ctcgccgtgt tcctcggcga ggtagcccat cttgccgaag ccgacgccgt gctcgccggt
     5101 gcaggtgccg cccatggcca gcgcgcgcag caccaggcgg tgtgacagcg cgtgcgcggc
     5161 ctcgcgttcg gcggcgctgt cggggtcggt cagcaccagc aggtggaagt tgccgtcgcc
     5221 gacgtggccg agcagcggca ccggcatcgg catgccggcg acatcggcgg ccgtggcctc
     5281 gatgcattcg gccagccgcg agatcggcac gcagacatcg gtggtcagcg agcgcgaacc
     5341 gggccgaagc tgcaggcagg cgaagtaggc atcgtggcgc gccgcccaga gctgcgtgcg
     5401 ttcttcctga cgcgtggccc agcggaagtc ggtgccgccg tgctcgtgcg ccagcgcctg
     5461 cacggtttcg gcctgttcgg cgacgccggc ttcggtgccg tggaactcga agaacagcgt
     5521 cggcacctcg ggccagttcg tcttcgaatg ggcgttgatc gcgcgcatcg acagcgcatc
     5581 gagcagctcg gcgcgcgcca ccggcacgcc ggtctggatg gtctggatca ccgtgcgcgc
     5641 cgccgaggcc acgtccggga agccgcagac cgcagccgag atcgcctcca gatgcgggtg
     5701 caggcgcacg gtgatctcgg tgacgatgcc gagcgtgcct tcggaaccga cgaacagccg
     5761 cgtcaggtcg tagccggccg ccgacttgcg cgcgcgcgtg ccggtgtgga tcacgcgccc
     5821 gtcggcgagc acgacggtca gcgccagcac cgcctcgcgc atcgagccgt agcgcacggc
     5881 gttggtgccg gaggcgcgcg tcgcggtcat gccgccgagg gtcgcatcgg cacccggatc
     5941 gatcgggaag aacagcccgg aatcgtgcag ccaggcgttg agctgcttgc gcgtgacgcc
     6001 ggcctgcacg gtggcgtcga gatcctcgac gtgcagcgcc aggatgcggt tcatgtgcga
     6061 caggtcgatg ctgacgccgc cgtgcagcgg gatcacgtgg ccttcgagcg agctgccgac
     6121 gccgaacggg atcatcggca cgcggaaggc atggcagagc cgcgcgatgg tcgagacttc
     6181 ctcggtggtt tcgggccaga ccacggcatc gggcagcgcc gccggatggc tcgattcatc
     6241 gcggccgtga tgctcgcgca ccgccagccc ggtcgagagg cgatcgccga gcagcgcacg
     6301 cagttcatcg agcagatcgg gtggcagggc gttggcgttg tacatggcgg caggctcggg
     6361 gcgcgcggat cgggcgaccg gctagtctac gcggccctga aaccgccggg cagggtcgca
     6421 cgtatcggtg accggcatcg ggcgtctgaa tctgctgaag cccgggacgc ctgccctcaa
     6481 cgcttgcggc acggtcgtgc cggggcatct ggtgcgtccg gcgtttgttc cggaccgccc
     6541 gcgcgttcac aatcgcgcgc tttacagctc aggcaccgtg cgccggtgct gccggagccg
     6601 taccgaatgt catccatcca tatgcttgac gccacgcgcg tcggggagac gcgccatgcc
     6661 tgaactcgaa ggcatcggcg gctggctgtt cgccgtgctg cagatcatcg ccatcgacat
     6721 cctgctcggc ggcgacaacg ccgtggtcat cgcgctcgcc tgccggcgcc tgcccgagga
     6781 caagcgccgg ctcggcgtgc gctggggcgt ggccggcgcc atcgtgctgc gcatcgtcat
     6841 gatcgcgttc gcgctgcagc tgctgctggt gccgggcctg aagctgatcg gcgcggcgct
     6901 gctgctgtgg atcggcatca agctgctgca gcccgaggcc gacgacggtc acgacggcat
     6961 cgagggcggc acgacgctgc ttgcggcggt gcgcaccgtc atcgttgccg atgcggtcat
     7021 gagcatcgac aacgtcgtcg ccgtcgccgc tgcggcgcgc ggcgagctgg cgctggtggt
     7081 gttcggcatc ctcgtcagcg tgccgatcat cgtctggggc agccacttcg tgctgcggct
     7141 gatggaccgc ttcccggtca tcatcgtgat cggcggggcg ctgctcggct ggatcgccgg
     7201 cgggctggcg gtccacgatc cgctgctcga acgcctcggg ttcgacgcgc cggactgggc
     7261 acactggccg gccagcctcg tcggggcgct cgccgtcgtg ctgatcggcc gccagctcgc
     7321 ggcgcgcgcc ggacgcgccg cgccggctcc gctcgaagac ctgctgtccg aagcgcgccg
     7381 cgacgaatga cgcccgccgc acccgatcgc ctgctgcccc cgggagttgc cgacatgagc
     7441 gtttcgaaga tcctggtgcc ggtggacggc acgcccgaaa gcgccgatgc ggtcgcgcac
     7501 gcgcttgcga tcgtcgcgga gcgcggcggc gagctgcatc tgctgcacgt gcgcctgccg
     7561 gtcgagtcct gccacgcgaa gctgctcgtc gacggccgca ccctgcatga ctggcaccgc
     7621 gaggagggcg agcaggcgct ggcgccgtcg ctcgcgcagt gccgggcggc cggcgtcgag
     7681 ccgctctgcc acgtcatcgt cggtcacctc gccgagacca tcgtgcgctt cgcgcacgaa
     7741 cagcacttcg gcctcgtcgt gatgcgccgc catccgcagg cggcgctgac gcacctgctg
     7801 ctcggctcgg tctgcgagga cgtgcaggcg cagtgccgca tcccggtcac cgtgctcgac
     7861 tgaacatggc gagccgccgg cgcctgctcg catggctcgc cgcgctgccg gccgcatggc
     7921 tggccgcgcg tgccgcggcc cgggagggcc ggccgctgac ggcctttgcg gcgacgagcc
     7981 tcgaggatgc gctgcgcgcg cagtacggcg atcgcccgcg cctgcgcgat gccgccctga
     8041 ggatcgaagc cccgcagagc gccgacaacg ccgcgcaggt gccgctgcgc atcgacgcgc
     8101 gcagccgccc ggtcctcgcg ctgacgctgt tcgccgagca caacccggtg ccgctgctcg
     8161 ggcgctggcg cttcggcgag ggcgtcgaag cctggctggc gctgcgcatc aagctcgctg
     8221 ccagcggccg ggtctgcgca gtcgccgagc tggccgacgg cgcgctgctg tacgccgaaa
     8281 cccgtgtcga ggtcggcacc ggcgcctgcg gctgacgacg atgagccccg atccggtccg
     8341 catcgaagcc cggtttctcg atggtcgcac cgaggtgcgg cttttgatcc gccacccgat
     8401 gcacaccggc cgcgagcgcg acccggacag cggcgccccg atcccggcgc tgtacatcga
     8461 cacgctgatc gtcgagcgga tcgcagccga cggccggacg cacgagctgc tgcaggccga
     8521 cttcggcccc ggcatcgccc gcaatccctt ccatcacttc gcgtttggcg gcggtgcgcc
     8581 cggggagacc gtgcgcgtgc gctggcgcga caccggcggc aacgaggcga gcggcgagac
     8641 cgtcatcacg gcctgatgcc ggtcacgcgt aaaggcatgg ttttcctgac tctctcccca
     8701 acccctctcc cgcagggggc gaggggctga aaagcggagt gctccgcagt gctcggcatg
     8761 agccggtcgt gcggcccgcg caagcccggc ttcagccgcg cagccattcg cccggctcga
     8821 cgcactcgta gtcgagcgcc tcggcgaccg cgcggcaggt gatgcgcccg gcatgcacgt
     8881 tcagcccggc catcaggccc gcatcgtcga acagcgcctg cgggaagccc ttgtcggcca
     8941 gtgccagcgt gtacggcagc gtggcgttgt tcagcgcgaa ggtcgaggtg cgcggcaccg
     9001 cgcccggcat gttggcgacg cagtaatgca cgatgccgtc gacgacgtag gtcggttcgg
     9061 cgtgcgtggt cgggtgcgag gtctcgaagc agccgccctg gtcgatggag acatcgacca
     9121 gcaccgcgcc ggggcgcatc tgtgcgagct gcgcgcgcgt gaccagcttc ggcgccgccg
     9181 cgcccggcac cagcaccgcc ccgaccacga gatcggattc gagcacctgc gcctcgatcg
     9241 cgtcgcgggt cgcgaacagc gtcgtcaggc gcgggccgta ttcgatgtcg agcgcgcgca
     9301 gccgatcgat gtcgcgatcg agcacggtga cgtgcgcgcc catgccgacc gccatgcgga
     9361 tcgcgccggc cccgaccacg ccgccgccga tcacggtcac ctgcgccggc gccacgccgg
     9421 gcacgccgcc gagcagcacg ccgcggccgc cgtgggcctt ttccagcgca tgcgcgccgg
     9481 cctggatcgc catgcggccg gcgacttcgg acatcggcgc gagcagcggc aggccgccgc
     9541 gtgcaccggt gacggtttcg taggcgatgg cggtgacgcc ggattcgacc agcagccggg
     9601 tctgttccgg gtccggggcc agatgcaggt aagtgaacag cagctgcccg gcgcgcaggc
     9661 gccggcactc gaccggctgc ggttccttga ccttcacgat cattgcggcg cgttcgaaga
     9721 cttccgcggc cgtcccgagg accgtggcgc ctgcggcgag gtactgcgca tcgtcgcagc
     9781 cgatgccgag tccggcatcg tgttcgacga ataccgtgtg gccgtgcgcg accagctcgc
     9841 gcacgctcgc gggggtcagt ccgacccggt attcgtggtt cttgatctct ttcgggacac
     9901 cgatctgcat ggcacgggca ctcctgcaat cgccggccgc acgggctgcg gtcgtggcgt
     9961 ggcggcaagc atgcgccgcg cgtttgcggc cggcagggcc ggctggcaag cagatttgac
    10021 ggctggacag attcatggcc agcgcgcacg gccgcgtggt cgcggcagtc cgcaagctgt
    10081 ccggcaagca ggggcgcttc cctgcgtggc aagggacgat cgatgcgcgg catctgcact
    10141 tcgatgcgcg gcaagggatg ccccctgcgc ggcaggcacg gatcgtcgat ccgcacggat
    10201 tcgcggctgc gatcggcagt ggcgggccac gacgcagcgc gggcggtcgt ggttgacgac
    10261 cgcccgcgca ggcgcggaag tggcggatcg gacagacaaa tgccggcctg caggcgctgg
    10321 cacggtccct gaagtgcctt gcctgtcccg tgcccggagt gtcgcccgat gccgaccgtc
    10381 cccgcgaaat cccgcagccc cgcgctgccg ccgcgcctgc tcgccgcgat ccgcgccgag
    10441 cagcaggcca gcgagcggct gatcggccag gtgcaactgg ccgtggtgct gaccttcggc
    10501 ctgctgtggg cgatcgcacc gagcccgccg agtccggcgc cgagtccggt gccgtgggcg
    10561 ctggccggct atctgctggt gacgctgacg cggctggcgt ggacgcagcg ccggcggctg
    10621 ccggactggg cggcgtacct gtcgatcgtc gccgacttcg cgctgctgta cgggctgatc
    10681 tggagcttcc acgtccagta catgcagccg ccggcgttct cgctgaaggc gccgacgctg
    10741 ctgtatgcgt tcatcttcgt cgtgctgcgc gtgctgcgcg tcgagctgcg cttcgtgatc
    10801 gcggccggtg cggtggcggt ggccggctgg gcggcgctgg tcgggctcgc gatcggcggc
    10861 aacctgcacg atgcggcgat cacgcgcgac tacgtgcatt acctgaccgc caacgccgtg
    10921 ctgctcggcg gcgagttcga caagctcgcg acgatgctga ccgtgacgct gatcctcgcg
    10981 ctcgcggtcg cgcgcgggca gcggctgctg gtgcgggcga cggtcgagtc caacgccgcg
    11041 atgcagctcg cgcgctttct gccgatggcg gtggccgtgc gcatcgccgc cgccaccgaa
    11101 cggccgccga ccggcgacgg cgagctggcg ccggcgacca tcctgttcct cgatctcgaa
    11161 ggcttcacga cgctggccga ggcgctgccg ccgccgcagc tgatcgcgct gctgaacgag
    11221 gtgttcgagg ccgtggtcgc gccgatcgag cgccacggcg gcgtcgtcga ccagttcatc
    11281 ggcgatgcgg tgatggcgac cttcaacctg ccgcagccgc acgccgatca cgcggccgcc
    11341 gcggtggcgg cggccctcga catccagacg ctgatggcga cgcaccgctt cgggaccggc
    11401 gtgcggctcg cggcgcgcat cggcatcaac accggcatcg tcgtcggcgg gctggtcggg
    11461 gccggcgagc ggctggcgta cacggtctac ggcgatgccg tgaacctcgc ggcgcgcctg
    11521 gagccgctga acaaggacta cggcacgcgc gtgctgctgt cggaggccac gcggctcgcg
    11581 gccggcgagg cgcgcttcgc gtggcgggcg gtcggcgagg tgcgcgtgcg cgggcgctcg
    11641 gtggcgacgc cgctgtatac ggtcgatgca cccgaagccg cggccccggc ggacgatgcg
    11701 ggctgagccg gggaggggtg ccgtcatgtc atcgtcaggc ttacacgatc gcttctgccg
    11761 gctctggcac gcgcacggcg gacgcgacgc cgggcaggtg ttcgccggac tcgccgcgca
    11821 ctacgccgag ccgcagcggc attaccacac gctcgcgcac atccgccgct gcctgcgcac
    11881 gctggatcat taccgcgcat cggtgccgca gcccgatctc gtcgagctgg cgctgtggtg
    11941 ccacgacgtg atctacgcac cggcggcccg cgacaacgag gcgagaagcg ccgactggtg
    12001 gcgggcgcag gctgcgggct tcgcgccggc cagcgtcgat ctggtcgccg acgccatcct
    12061 cgctaccacg catcgccagc cgccgacggg cgcagtcgcg gcctggaccg tcgacatcga
    12121 tctggccgga ctggcgctgt cgcgatcgag cttcgcgcgc gacggcagga agctgcgggc
    12181 cgagcgaccg gaactcgggg aggccgaata cggtgcgggg gagcgggcgt ttctgtcggg
    12241 gctgctggcg cgggcgcgta tctattacac ggaagcgctg tatgcagatg gcgaggcgcg
    12301 ggcgcgggaa aacctgcggc ggcggatcgc gacgctgaca ggggcacggc atgccggagc
    12361 ggccggcggg ctttcagccc ttgggacggg ctgacgaagg aagcccctcg ctcgcgcagt
    12421 cggccgcagc actgtttgac agcttcggcc ggctcctcgc cagcgccggc agcctgccga
    12481 cctacggcta cagcggccgc gagcccgacg ccaccggcct cgtctactac cgcgcccgct
    12541 actacgcccc ggagcttgga cgcttcacgc agccggaccc ggtggggttg gccgggggga
    12601 tcaaccggta tgcgtacgtg ggagggaacc cgattgattt cagggatccg agcgggttcg
    12661 tcgtcgcacc gccggtgtcg ccgggcaacg ccctgcccgc ttatgccggc gccgtcggcg
    12721 gcgtggcggt gacagccgcg gagactggcg ctgccggcct ggagggctcc gggtttctca
    12781 cgggtgccgc gagcggggtc agaacattgc tgacctacgc cggccgcgga ctcgacccga
    12841 tggccgaggc tactgaatgt cgtggttcaa tttccgtgga cacccggata ggggaaatcc
    12901 ccccaccgag a
//
LOCUS       DAOQIE010000071        12887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2375_length_12887_cov_8.279847, whole genome shotgun sequence.
ACCESSION   DAOQIE010000071 DAOQIE010000000
VERSION     DAOQIE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_12887_cov_8.279847"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PK144_06280"
     CDS             <1..1823
                     /locus_tag="PK144_06280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65371.1"
                     /translation="VSADRAAEFGIQWQGGVPKDNSTTAYGGTNFGSGGQNILNLAQN
                     PLAAASGMNFLVGAGPVTINGTKIFNLNLLARFLESDARANILSTPSLMTVDNEEAKI
                     VVGRNVPFVTGTYSNTSGSSSTVSPFNTYERKDVGLTLKIKPQITEGGTIRMQIFQET
                     SAVIDSTSSSSSGPSTTKRSVESTVITDDGSIIAIGGLVEDSYAGGVDKVPLLGDIPI
                     LGALFRYDTRKRVKTNLMVFLRPRELRRAAELLEAKVSARTAELNDANRALAQRAAES
                     ERRAHEAGQLATLSNRLQLADAPASAYTVLADAMPALFPGCPGAFYRLAGDGALQALA
                     VWGGLAAQATAVASGRCRALQDGELHCHTLAAGTRACAALSTGSTGYVCMPVLAQGGT
                     LGLLHLQFDTHDVREHEAVARLARELAERLGLTLANLELRESLRHESLHDPLTGVCNR
                     RYMEDCLRRELVRIARDGHVLSVLMVDIDHFKAVNDRWGHEAGDRTLRATARRLGRGT
                     RGGDVVCRYGGEEFVVILPGADLTDAARRAEQLRAEAESGADAGEEPGAQPVTLSIGV
                     ASAPLHGRSSDALLGAADRALYQAKSAGRNRVVIAAEPAA"
     gene            complement(1839..2879)
                     /locus_tag="PK144_06285"
     CDS             complement(1839..2879)
                     /locus_tag="PK144_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HNF65372.1"
                     /translation="MRTVTLGCTDLVVSRACLGTMTWGEQNTEAEARAQLDFATARGV
                     NFIDTAEMYPVPPRAETQGRTEQYLGNWLAAQPGRRNRLVIASKVAGASRGIDWLRPG
                     PLRLDRANVLAACEASLKRLRTDHLDLYQVHWPDRPTNMFGQLGYRVDDTPAVPVEET
                     LAALAELVQAGKVRHVGISNETPWGLMRYLRAAETPGLPRIASIQNPYSLLNRSFEIG
                     LAEMSWCEQVPLLAYSPLAMGVLSGKYLDGARPAGARLSRFERFTRYSQPEAEAPTRA
                     YVGLARQHGLDPAAMALAWVDRQPWVGATIIGATSLAQLEADLAAFDTPLDAAVCAAI
                     DAIHQRQPNPCP"
     gene            complement(3001..3690)
                     /gene="trmB"
                     /locus_tag="PK144_06290"
     CDS             complement(3001..3690)
                     /gene="trmB"
                     /locus_tag="PK144_06290"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNF65373.1"
                     /translation="MNDVENPRRIRSFVRREGRLTLAQQRALDELWPRFGIDFSPAAL
                     DLQSLFGRTALRVLEIGFGNGESLAAMAAAAPYTDFIGIEVHRPGVGHLLLAIERLAL
                     QNLRVCCHDAVEVLERQIPDASLDRVHIFFPDPWHKKRHHKRRLIQPPFVALLARKLK
                     PGGHLHLATDWEDYAVHMLAVLGQSADFANSAPAGEMFCPRPDYRPLTKFERRGERLG
                     HGVRDLIFERT"
     gene            complement(3760..4569)
                     /locus_tag="PK144_06295"
     CDS             complement(3760..4569)
                     /locus_tag="PK144_06295"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNF65374.1"
                     /translation="MSASTDQTASTPAPLVIAGREYRSRLLVGTGKYRDLDETRRATE
                     ASGAEIVTVAIRRTNIGQTPGEPNLLDVLPPDRYTILPNTAGCFDADSAVRTCRLARE
                     LLDGHDLVKLEVLGDQKTLFPDIVETLKAAEILIRDGFKVMVYTNDDPLIAKRLETMG
                     CVAVMPLAAPIGSGLGVRNPYNILTIVENAQVPVLVDAGVGTASDAAIAMELGCDGVL
                     MNTAIAGARNPVLMASAMKKAVEAGREAWLAGRIPKKRFASASSPLDGLFF"
     gene            complement(4644..4844)
                     /gene="thiS"
                     /locus_tag="PK144_06300"
     CDS             complement(4644..4844)
                     /gene="thiS"
                     /locus_tag="PK144_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNF65375.1"
                     /translation="MQIKVNGQPYEAEDSLTAAALVERLGYAGRRLALEINREIVPRS
                     RWSQTPLQADDEVELVHAIGGG"
     gene            5331..5684
                     /locus_tag="PK144_06305"
     CDS             5331..5684
                     /locus_tag="PK144_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNF65376.1"
                     /translation="MSVRFEDIDVSTFNLEQLQALMEMARKEIEVKEQTRLLEVAAQI
                     QSLASGVGRSVEEILSMSSDGKRKRTSKSDTGEKIVKFRNTVNPDETWSGRGKRPRWL
                     QEALLNGADIQSFAV"
     gene            5737..5810
                     /locus_tag="PK144_06310"
     tRNA            5737..5810
                     /locus_tag="PK144_06310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5769..5771,aa:Gly,seq:ccc)
     gene            5969..6772
                     /gene="dapB"
                     /locus_tag="PK144_06315"
     CDS             5969..6772
                     /gene="dapB"
                     /locus_tag="PK144_06315"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNF65377.1"
                     /translation="MIRIAIAGVAGRMGRQLVEACAQAADVTLAVAVERPGSSMIGCD
                     AGELAGIGRNGVVVGDDLAARLDDFDVLIDFTRPEPTLAHLALCRAAGRCAVIGTTGF
                     TAAQKAEIQAAAADTGIVFAPNFSVGVNLCFRLLDLAARVLGDEVDIEVLEAHHRHKV
                     DAPSGTALRMGEVVAQALGRELSEVAVYGREGQTGERDRRTIGFATVRAGDIVGEHTV
                     LFADIGERVEITHKASSRMTFARGAVRAAGWLAGRAPGLYDMQDVLGLR"
     gene            6930..8066
                     /gene="carA"
                     /locus_tag="PK144_06320"
     CDS             6930..8066
                     /gene="carA"
                     /locus_tag="PK144_06320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNF65378.1"
                     /translation="MLRKPALLALADGSLFRGESIGADGSTTGEVVFNTSITGYQEIL
                     TDPSYCRQIVTLTYPHIGNVGANPGDEEAAKIWSSGLVVRDCPPRPSSWRSRQSLDAY
                     LSERGVVAIAGIDTRRLTRHLREQGAQNGCIVAGDRPDAEAAIAAARAAPKMVGLDLA
                     QVVTTESRYEWTEGCWRLDGDDFPQQAEARFHVVAYDYGVKRNILRMLAARGCRVSVV
                     PARTPAAEVLALRPDGVFLSNGPGDPEPCDYAITAIRELLDAGVPIFGICLGHQLLGL
                     ASGARTVKMKFGHHGGNHPVQDLDSGRVMISSQNHGFAVDEATLPATLRATHRSLFDG
                     TLQGIERTDRPAFSFQGHPEASPGPHDVAGLFDRFIGLMQRHGR"
     gene            8164..11391
                     /gene="carB"
                     /locus_tag="PK144_06325"
     CDS             8164..11391
                     /gene="carB"
                     /locus_tag="PK144_06325"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNF65379.1"
                     /translation="MPKRTDIQSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADAIYIEPIHWQTVARIIEQERPHALLPTMGGQTALNCALDL
                     AHHGVLEKFGVEMIGANEDAIDMAEDRERFRDAMTEIGLATPRARIAHTIEEAVAAQA
                     DIGFPTIIRPSFTMGGSGGGIAYNRQEFLEIVERGLDLSPTSEVLLEESVLGWKEYEM
                     EVVRDRVDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDREYQIMRDASLAVLRKIG
                     VDTGGSNVQFGLNPADGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITGGLTPASFEPSIDYVVTKVPRFNFEKFPQADARLTTQMKSVGEIMAIGRTFQ
                     ESVHKALRGLEIGTDGFNPLLDRLDPEAPLRLKQELGVPGAHRIWYVADAFRLGYTVE
                     QVHDYTRIDPWFLDQIAELIELEAGIAGHGVAGLRERDRLYGLKRKGFSDRRLAALTG
                     TNEHEIRTLRTSLNVHPVYKRVDSCAAEFATGTAYLYSTYEEECEAAPSARDKIMILG
                     GGPNRIGQGIEFDYCCVHAALSLREDGYETIMVNCNPETVSTDYDTSDRLYFEPLTLE
                     DVLEIVRVEAPKGVIVQYGGQTPLKLARPLEAAGVPIIGTSPDAIDRAEDRERFQRMI
                     DELALRQPPNRTARDPETAVRLAAEIGYPLVVRPSYVLGGRAMEIVHNEDDLRRYMRE
                     AVQVSNDSPVLLDRFLNDAIEVDVDAIADADGDVVIGGIMEHIEEAGVHSGDSACSLP
                     PYSLSPLTLDRLRTQVRAMARELGVVGLMNTQFAIQGDDIFVLEVNPRASRTVPYVSK
                     ATGRPLAKIAARCMAGRSLAGQGVQGEIVPSYYSVKEAVFPFVKFPGVDPLLGPEMKS
                     TGEVMGVGRSFGEAYAKAQLGAGNRLPASGKAFLSVREVDKVKIVEVARDLVARGFSL
                     VGTRGTAATLNAAGLECAIVNKVGEGRPHIVDMIKNDEISLIVNTTEGKQAIADSFSL
                     RRNALMHKVSYTTTIAAARASVMALEDLGSDRVTRLQDLHKELQA"
     gene            11388..11867
                     /gene="greA"
                     /locus_tag="PK144_06330"
     CDS             11388..11867
                     /gene="greA"
                     /locus_tag="PK144_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNF65380.1"
                     /translation="MTQKVPMTATGAEKLRAELQELKTVARPKVIEAIAEARAHGDLK
                     ENAEYSAAREQQGFIEGRIKEIEYKLSHAHVIDVTALPRTGKVVFGVTVTLADEDSGD
                     EVRYQIVGEDEADIPSGRISVNSPIARALIGKEEGEVATVQTPGGVRHWEVVAVEHL"
     gene            11967..12749
                     /locus_tag="PK144_06335"
     CDS             11967..12749
                     /locus_tag="PK144_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNF65381.1"
                     /translation="MYRCLTRATLLAILALSGLLPAAVPAASLPDTIDAIRGAVVAVG
                     TYEATRRPPANFLGTGFVVGDGTWVITNAHVVDKPLDPVKREFPAVFAGRGNEFATIE
                     AKIVQTDPEHDLALLKLLGAPLPTRFRLAGGLPREGQEIAFTGFPIGVVLGLYPVTHR
                     GIVSAITPVAIPAPSAGQLDANAIRRLSRPFNVLQLDATAYPGNSGSPLYDPASGRVY
                     GVINSVFIKETKENVIQKPSGITYAIPVEYLQALMRKAGAGN"
     gene            12797..12871
                     /locus_tag="PK144_06340"
     tRNA            12797..12871
                     /locus_tag="PK144_06340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12829..12831,aa:Val,seq:cac)
BASE COUNT         2151 a         4394 c         4350 g         1992 t
ORIGIN      
        1 aggtgtccgc ggaccgtgcc gccgagttcg gcatccagtg gcaagggggc gtccccaaag
       61 acaactcgac gacagcctac ggcggaacaa acttcggttc tggcgggcag aatatcctga
      121 accttgccca gaacccgctt gcagcagcgt cgggcatgaa cttcctggtc ggtgccggcc
      181 ccgtgacgat caacgggacc aagatcttca atctcaacct gcttgcgcgt tttctggagt
      241 cagatgctcg cgcaaacatt ctgtccaccc ccagcctgat gacggtcgac aacgaagaag
      301 cgaagattgt cgtcgggcga aatgtgccct tcgtaaccgg cacctactcc aacaccagcg
      361 gttcgtcgag cacggtcagc cccttcaata cctatgagcg caaagacgtt ggcctgaccc
      421 tgaaaatcaa gccccagatc accgaaggcg gcacgattcg gatgcagatc ttccaggaga
      481 cctctgcggt cattgactcg accagttcgt cctcgagtgg cccctccacg accaaacgct
      541 cggttgaatc gacggtcatc accgacgatg gaagcatcat tgcgattggt ggtctggtcg
      601 aagacagcta cgctggcggg gtcgacaaag taccgcttct cggcgacatc ccgattcttg
      661 gagcgctttt ccggtatgac accagaaaac gggtcaagac aaacctgatg gtgtttctcc
      721 gcccgcgcga actgcgccgc gccgccgagc tgctcgaagc caaggtcagt gcgcgcaccg
      781 ccgagctgaa cgacgccaac cgcgccctcg cgcagcgcgc cgccgaatcc gaacgccgtg
      841 cccacgaggc cggccagctc gcgacgctca gcaaccgcct gcagctcgcc gatgccccgg
      901 ccagcgccta caccgtgctc gccgatgcga tgccggccct gttcccgggc tgcccggggg
      961 ctttctaccg gctcgcgggc gacggcgcgc tgcaggccct ggcggtctgg ggcgggctgg
     1021 cggcgcaggc cacggcggtc gccagcggtc gctgccgggc gctgcaggac ggcgagctgc
     1081 actgccacac gctcgccgcc ggcacgagag cctgtgcggc gctttcgacc ggcagtaccg
     1141 gctacgtgtg catgccggtg ctcgcccagg gcggcaccct cggcctgctg cacctgcagt
     1201 tcgacaccca cgatgtgcgc gagcacgagg cggtcgcgcg cctggcgcgc gagctggccg
     1261 agcgtctggg gctcacgctc gcgaatctcg aactgcgcga atcgctgcgc cacgaatcgc
     1321 tgcacgaccc gctgaccggc gtctgcaacc gtcgctacat ggaagactgc ctgcgccgcg
     1381 agctggtgcg catcgcccgc gacggccacg tgctgtcggt gctgatggtc gacatcgacc
     1441 acttcaaggc cgtcaacgac cgctggggcc acgaggccgg cgaccggacg ctgcgcgcca
     1501 cggcccgccg gctcggccgc gggacgcgcg gcggcgacgt ggtctgccgc tacggcggcg
     1561 aggagttcgt cgtgatcctg ccgggcgccg atctcaccga tgccgcacgc cgtgccgagc
     1621 agctgcgcgc cgaggccgag tccggggccg atgccggcga ggagccgggc gcgcagccgg
     1681 tgacgctgtc gatcggggtg gcgagcgcgc cgctgcacgg gcgcagttcc gatgcactgc
     1741 tcggtgccgc cgaccgcgcg ctgtaccagg ccaagagcgc cggccgcaac cgcgtggtga
     1801 tcgccgcgga gccggccgcc tgaacggcgc gctggcgctt acggacaggg gttcggctgg
     1861 cgctggtgga tggcgtcgat cgcggcgcag acggctgcat cgagcggcgt gtcgaaggcg
     1921 gcgaggtcgg cttcgagctg cgcgagcgag gtcgccccga tgatcgtcgc gccgacccag
     1981 ggctgacggt cgacccaggc gagcgccatc gccgccgggt cgaggccgtg ctgccgggcc
     2041 agcccgacgt aggcgcgggt cggggcttcg gcctccggct gcgaatagcg cgtgaagcgc
     2101 tcgaaccggc tcagccgcgc cccggccggt cgcgcgccgt cgaggtactt gccgctcagc
     2161 acgcccatcg ccagcggcga atacgcgagc agcggcacct gctcgcacca gctcatctcg
     2221 gccaggccga tctcgaagct gcggttgagc aggctgtacg ggttctggat gctggcgatg
     2281 cgcggcagcc ccggggtttc ggcggcgcgc aggtagcgca tcagccccca cggcgtctcg
     2341 ttggagatgc cgacgtggcg caccttgccg gcctgcacga gttcggcgag cgcagccagg
     2401 gtttcctcga ccggcaccgc cggcgtgtcg tcgacgcggt agccgagctg gccgaacata
     2461 ttggtcgggc ggtccggcca atgcacctgg tagaggtcca ggtgatcggt gcgcaggcgt
     2521 ttcaggctcg cctcgcaggc cgcgagcacg ttggcgcgat cgagcctgag cggccccggc
     2581 cgcagccagt cgatgccgcg cgaggcaccg gcgaccttgc tcgcgatcac caggcggttg
     2641 cgccggccgg gctgcgcggc gagccagttg ccgaggtact gctcggtgcg cccctgggtt
     2701 tcggcgcgcg gcggcaccgg gtacatctcg gccgtgtcga tgaagttcac gccgcgggcc
     2761 gtcgcgaaat cgagctgcgc gcgggcttcg gcctcggtgt tctgctcgcc ccaggtcatc
     2821 gtgccgagac aggcgcggct gacgacgaga tcggtgcaac ccagggtcac ggtgcgcatg
     2881 cgcggaggct ccggcggtcg ggatcggaag ccggggaacg taacccgcag gaggtatcga
     2941 tccccatgcc tgcatgcgtg gcatgcacgg agcgacgggc cgggccgcgg ccggtgctgc
     3001 tcaggtccgc tcgaagatca gatcccgaac cccgtggccg agccgctcgc cgcggcgctc
     3061 gaatttggtc agcggccggt aatccgggcg cggacagaac atttcaccgg cgggcgcgga
     3121 attggcgaaa tccgccgact ggccgagcac ggcgagcatg tggacggcgt aatcctccca
     3181 gtcggtggcc aggtgcagat ggccgccggg cttgagtttg cgggccagca gcgccacgaa
     3241 cggcggctgg atcagccggc gtttgtggtg gcgcttcttg tgccagggat caggaaagaa
     3301 aatgtggacg cgatcgagac ttgcatccgg aatctgtcgt tccagcacct cgacggcgtc
     3361 atggcagcaa acccgcaggt tctgcagggc caggcgttcg atcgcgagca gcaggtgtcc
     3421 gaccccgggc cggtggacct cgatgccgat gaagtcggta tagggcgccg ccgccgccat
     3481 cgccgccagc gattccccgt tgccgaaacc gatttccagc acgcgcagcg ccgtgcggcc
     3541 gaacagggat tgcaggtcga gtgccgcagg actgaagtcg atgccgaatc gcggccagag
     3601 ttcatcgagg gcacgttgct gcgcgagagt cagccggcct tcccggcgca cgaaactgcg
     3661 gatgcgccgg ggattttcga cgtcgttcat gatcggttgg gagggtagaa aacttttttg
     3721 caggcgccgc cgccgcgatg ggcgcagcgg cggcggggat cagaaaaaca ggccatcgag
     3781 cggcgaggat gcgctcgcga aacgcttttt cggaatgcgt ccggccagcc aggcctcgcg
     3841 accggcttcg acggcctttt tcatcgcact cgccatcagc accggattgc gggctccggc
     3901 gatcgcggtg ttcatcaata cgccgtcgca gccgagttcc atggcgatcg ccgcatccga
     3961 agccgtgccg acgccggcat cgaccagcac cggcacctgt gcattctcga cgatggtcag
     4021 gatgttatac ggattgcgca cgccgaggcc cgaaccgatc ggcgcggcca gcggcatcac
     4081 cgccacgcag cccatggttt caaggcgctt cgcgatcagc gggtcgtcat tcgtgtagac
     4141 catgaccttg aagccgtcgc gaatcaggat ttccgcggcc ttcagggttt cgacgatgtc
     4201 cggaaacagc gtcttctgat cgccgagcac ttcgagcttg accagatcgt ggccatcgag
     4261 cagctcgcgc gcgagccggc aggtgcgcac ggcgctgtcg gcgtcgaagc agccggcggt
     4321 gttcggcagg atcgtgtagc gatccggcgg cagcacgtcg agcaggttcg gctcgccggg
     4381 cgtctggccg atgttggtgc gccggatcgc gaccgtgacg atttcggcgc cgctcgcctc
     4441 ggttgcccgc cgggtctcgt ccagatcgcg gtatttgccg gtgccgacca gcaggcgcga
     4501 gcggtattcg cgaccggcaa tcaccagcgg ggcaggggtg gaagcggttt gatcggtact
     4561 tgcggacatg ggcggactcg ggcgcagtga ggacgcggtg aggaagggac ggattgcggc
     4621 cgcaggacgg gacgggtgtc gattcagccg ccaccgatcg cgtggacgag ttcgacctcg
     4681 tcatccgcct gcagcggcgt ctgcgaccag cggctgcgtg gcacgatttc gcgattgatc
     4741 tccagtgcca gacgcctgcc ggcgtatccg aggcgttcga cgagcgcggc cgccgtcagg
     4801 gaatcttcgg cttcgtaagg ctggccgttg accttgatct gcatgatgag gaatgcatcc
     4861 ggtcgggcag ggacggggcg gcgatcatac acgatcgcgc gtgcctgaca gtacaagtgc
     4921 ggaccgctgc cggcaatcgc ggctgccaag caaggggcca tggacggtaa cgacctgatg
     4981 catcgaagtg aaatgcagac cgtgacaacg gcttgcggtt cccggcaccc ggcgactgcg
     5041 acaatgccgg tgtcgccgaa gtaccggatc acaaagccga tggctgcact tacgcatgac
     5101 agccgccccg gcaggtgccc gaatgtgtcg gcggtcagcc gtggcgttac acggatacgc
     5161 taatgcagca tgaaatttag cagggtcgat actggcaagt ccgatattca tgaattacca
     5221 tgcctgacat ccgcagtgtc ggattgatcc ggaagcagcc gctacgtgac ccgccaggac
     5281 gagcctgctc ccgcaacatt tacgatgcac ccagccgaga gatcattccc atgagcgtac
     5341 gcttcgaaga catcgatgtt tcgaccttca acctcgaaca actgcaggcg ctgatggaga
     5401 tggcgcgcaa ggaaattgaa gtcaaggagc agacccggct gctggaagtc gctgcgcaga
     5461 ttcagagtct ggccagcggc gtcggccgca gcgtcgagga aatcctgtcg atgtccagcg
     5521 atggcaagcg caagcgcacc agcaagtcgg ataccggtga aaaaatcgtc aagttccgca
     5581 acaccgtgaa tcccgacgaa acctggagcg gtcgcggcaa gcgcccgcgc tggctgcagg
     5641 aagccctgct gaacggtgcc gacatccaga gttttgccgt ctgagcaggc ccggggcttg
     5701 ccgaatgccg tacaggcggc ataataggcg ggcccagcgg gtgtagttca atggcagaac
     5761 ctcagcttcc caagctgatg gcgtgggttc gattcccatc acccgctcca gaaccgcaag
     5821 ggccgcgcca gtcgcggccc ttttcatttc cgagcatcgc tcgcgccgcc gtccgtgaat
     5881 tcggggcacc cggggcagcc aggggcaagc ttctgaatca tcgcgaatcc ggaccggctg
     5941 cgggatgcca gatcgaggcc gccagcccat gatcagaatt gcgattgccg gcgtcgccgg
     6001 gcgcatgggg cgccagctcg tcgaggcctg tgcgcaggct gcggacgtga cgctcgccgt
     6061 cgccgtcgag cggcccggca gcagcatgat cggctgcgat gccggggaac tcgccggcat
     6121 cgggcgcaac ggcgtcgtcg tcggtgacga ccttgcggcc cggctcgatg acttcgacgt
     6181 gctgatcgac ttcacgcgcc cggagccgac gctcgcgcat ctggcgctct gtcgcgcggc
     6241 cgggcgctgc gccgtgatcg gcacgaccgg cttcacggcg gcgcagaagg cggagatcca
     6301 ggccgccgcc gccgataccg gcatcgtgtt cgcgccgaac ttcagcgtcg gtgtgaatct
     6361 ctgtttcagg ctgctcgacc tcgctgcccg cgtgctcggc gatgaggtcg atatcgaagt
     6421 gctcgaagcc catcaccggc acaaggtcga tgcgccctcg ggcacggcgc tgcgcatggg
     6481 cgaggtggtc gcgcaggcgc tcgggcgcga gctgtccgaa gtcgccgtat acggccgcga
     6541 gggccagacc ggcgagcgcg accggcgcac gatcggcttc gcgaccgtgc gcgccggcga
     6601 catcgtcggc gagcacacgg tgctgttcgc cgacatcggc gagcgtgtcg agatcacgca
     6661 caaggcgtcg agccgcatga ccttcgcgcg cggtgcggtg cgcgccgccg gctggctggc
     6721 cgggcgcgct ccggggctct acgacatgca ggacgtgctc ggcctgcgct gagccgggca
     6781 ccgtcgtcat gcgtcgtcat ggacggaatt gctgccctgc ggtagatttc cggcactttg
     6841 actgagattg acgaaaacgt aatcctcgga caccgggcgg gagggaaggc aacgtttccc
     6901 tcccgccttc gcacgtctga atggaggaaa ttttgaggaa acccgcactg ctggccctgg
     6961 cagacggcag cctgttccgg ggtgagtcca tcggtgccga tggttcgacc accggcgaag
     7021 tcgtgttcaa cacctcgatc accggctatc aggagatcct caccgacccc tcgtactgcc
     7081 ggcagatcgt gacgctgacc tatccgcaca tcggcaacgt cggtgccaat cccggcgacg
     7141 aggaagcggc gaagatctgg tcctcggggc tggtcgtgcg cgactgcccg ccgcgtccga
     7201 gcagctggcg cagccgccag tcgctcgatg cctatctgag cgagcgcggc gtggtcgcga
     7261 tcgccggcat cgacacgcgc cggctgacgc gccacctgcg cgagcagggc gcgcagaacg
     7321 gctgcatcgt cgccggcgac cggccggatg ccgaggccgc gatcgccgcg gcgcgcgccg
     7381 cgccgaagat ggtcgggctc gacctcgcgc aggtggtgac gaccgaaagc cgctacgagt
     7441 ggaccgaagg ctgctggcgg ctcgacggcg atgacttccc gcagcaggcc gaggcgcgct
     7501 tccacgtcgt cgcctatgac tacggcgtca agcgcaacat cctgcgcatg ctcgccgcgc
     7561 gcggctgtcg cgtcagcgtc gtgccggcgc gcacgccggc ggccgaggtg ctggccctgc
     7621 gccccgatgg cgtgttcctg tccaacggtc cgggcgatcc cgagccctgc gactacgcga
     7681 tcacggcgat ccgcgagctg ctcgacgccg gcgtgccgat cttcggcatc tgtctcggcc
     7741 accagctgct cggactggcc tccggcgcgc gcacggtcaa gatgaagttc ggccaccacg
     7801 gcggcaatca tccggtgcag gatctcgatt ccggccgcgt gatgatttcc agccagaacc
     7861 acggcttcgc ggtcgatgaa gccacgctgc cggccacgct gcgcgccacg caccgttcgc
     7921 tgttcgacgg cacgctgcag ggcatcgagc gcaccgaccg gccggcgttc agcttccagg
     7981 gccatccgga agcgagtccc gggccgcacg acgttgccgg cctgttcgac cgcttcatcg
     8041 gcctgatgca acgccacggc cgctgagcct gcctgaggcc ggcgcccgtg cgtgcgcacg
     8101 gggcgccggc acgcccgaat ctcccgaacg cctcgctgcg tctgctgccc acggtttccg
     8161 tccatgccga aacgtaccga catccagagc atcctgatca tcggcgccgg tcccatcgtg
     8221 atcggccagg cctgcgagtt cgactactcg ggcgcccagg cctgcaaggc gctgcgcgag
     8281 gagggctacc gcgtcatcct cgtcaattcc aatccggcga cgatcatgac cgacccggag
     8341 atggccgacg cgatctacat cgagccgatc cactggcaga cggtcgcgcg catcatcgag
     8401 caggagcgcc cgcatgcgct gctgccgacc atgggcgggc agacggcgct gaactgcgcg
     8461 ctcgatctgg cgcaccatgg cgtgctcgaa aaattcggcg tcgagatgat cggcgccaac
     8521 gaggacgcca tcgacatggc cgaggaccgc gagcgcttcc gcgacgcgat gaccgagatc
     8581 ggtctggcga cgccgcgcgc gcgcatcgcg cacacgatcg aggaggccgt cgccgcgcag
     8641 gccgacatcg gcttcccgac catcatccgg ccgtcgttca cgatgggcgg ctcgggcggc
     8701 ggcatcgcct ataaccgcca ggaattcctg gagatcgtcg agcgcggcct ggacctgtcg
     8761 ccgaccagcg aggtgctgct cgaagagtcg gtgctcggct ggaaggaata cgagatggag
     8821 gtcgtgcgcg accgcgtcga caactgcatc atcgtctgct cgatcgagaa cttcgatccg
     8881 atgggcgtgc acaccggcga ctcgatcacc gtcgcaccgg cgcagacact gaccgaccgc
     8941 gaataccaga tcatgcgcga cgcctcgctg gcggtgctgc gcaagatcgg cgtcgacacc
     9001 ggcggctcga acgtgcagtt cggcctcaat cccgccgacg ggcgcatggt cgtcatcgag
     9061 atgaacccgc gcgtgtcgcg ctcctcggcg ctcgcttcga aggccaccgg cttcccgatc
     9121 gccaaggtcg ccgccaagct cgcggtcggc tacacgctcg atgagctgaa gaacgagatc
     9181 accggcgggc tgacgccggc ctcgttcgag ccgtcgatcg attacgtcgt caccaaggtg
     9241 ccgcgcttca acttcgagaa gttcccgcag gccgacgcgc gcctgaccac gcagatgaag
     9301 tcggtcggcg agatcatggc gatcggccgc accttccagg agtcggtgca caaggcgctg
     9361 cgcggcctgg agatcggcac cgacggcttc aatccgctgc tcgatcgcct cgatcccgag
     9421 gcgccgctgc gcctgaagca ggaactcggc gtgcccggcg cgcaccgcat ctggtacgtc
     9481 gccgatgcct tccggctcgg ctacacggtc gagcaggtgc acgactacac gcggatcgac
     9541 ccgtggttcc tcgaccagat cgccgagctg atcgaactcg aggccggcat cgccggccac
     9601 ggcgtcgccg gcctgcgcga gcgcgatcgc ctgtacggcc tcaagcgcaa gggcttctcg
     9661 gaccggcgcc tcgcggcgct gaccggcacg aacgagcacg agatccgcac gctgcgcacc
     9721 agcctgaacg tgcatccggt ctacaagcgc gtcgactcct gcgccgccga gttcgcgacc
     9781 ggcaccgcgt atctctattc gacctacgag gaggaatgcg aggccgcccc gagtgcgcgc
     9841 gacaagatca tgatcctcgg cggcgggccg aaccgcatcg gccagggcat cgagttcgac
     9901 tactgctgcg tccacgccgc gctgtcgctg cgcgaggacg gctacgagac gatcatggtc
     9961 aactgcaacc ccgagaccgt ctcgaccgac tacgacacct cggaccgcct gtacttcgag
    10021 ccgctgacac tggaggacgt gctggagatc gtgcgcgtcg aggcgccgaa gggcgtgatc
    10081 gtgcagtacg gcggccagac gccgctgaag ctcgcgcgtc cgctcgaagc cgccggcgtg
    10141 ccgatcatcg gcaccagccc cgatgccatc gaccgcgccg aggatcgcga gcgcttccag
    10201 cgcatgatcg acgaactggc gctgcgccag ccgccgaacc gcaccgcgcg cgatcccgag
    10261 accgccgtgc gcctggccgc cgagatcggc tatccgctgg tcgtgcgtcc gtcctacgtg
    10321 cttggcggcc gggcgatgga gatcgtccac aacgaggacg acctgcgccg ctacatgcgc
    10381 gaggccgtgc aggtcagcaa tgacagtccg gtgctgctcg atcgcttcct gaacgacgcg
    10441 atcgaggtcg atgtcgacgc gatcgccgac gccgacggcg atgtcgtcat cggcggcatc
    10501 atggagcaca tcgaggaggc cggcgtgcac tcgggcgatt cggcctgctc gctgccgccg
    10561 tactcgctgt cgccgctgac gctcgaccgg ctgcgcacgc aggtgcgggc gatggcgcgc
    10621 gagctcggcg tcgtcgggct gatgaacacg cagttcgcga tccagggcga cgacatcttc
    10681 gtgctcgaag tcaatccgcg cgcctcgcgc accgtgccct atgtttccaa ggccaccggc
    10741 cggccgctcg cgaagatcgc cgcgcgctgc atggccgggc gctcgctggc cgggcagggc
    10801 gtgcagggcg agatcgtgcc ctcgtactac tcggtcaagg aagccgtgtt cccgttcgtg
    10861 aagttcccgg gcgtcgatcc gctgcttggc cccgagatga agtcgaccgg cgaggtcatg
    10921 ggcgtcgggc gcagcttcgg cgaggcctac gccaaggccc agctcggtgc cggcaaccgc
    10981 ctgccggcct ccggcaaggc cttcctgtcg gtgcgcgagg tcgacaaggt caagatcgtc
    11041 gaggtggcgc gcgatctggt cgcccgcggc ttctcgctgg tcggcacccg cggcacggcg
    11101 gcgacgctca acgccgccgg gctggagtgc gccatcgtca acaaggtcgg cgaaggccgg
    11161 ccgcacatcg tcgacatgat caagaacgac gagatctcgc tgatcgtcaa caccaccgaa
    11221 ggcaagcagg ccattgccga ctcgttctcg ctgcgacgca atgcgctgat gcacaaggtc
    11281 agctacacca ccacgatcgc cgccgcgcgc gcctccgtca tggcgctcga agacctcggc
    11341 agcgatcgcg tcacccgcct ccaggatttg cacaaggagc tccaggcatg acccagaagg
    11401 tgccgatgac tgccaccggc gcagaaaagc tcagggccga actgcaggaa ctgaaaaccg
    11461 ttgcccgccc gaaagtcatc gaggcgatcg ccgaggcgcg cgcacacggg gacctgaagg
    11521 aaaacgccga atattccgcc gcgcgcgagc agcagggctt catcgaaggc cgcatcaagg
    11581 aaatcgaata caagctctcg catgcgcacg tcatcgacgt gaccgcgctg ccgcgtaccg
    11641 gcaaggtcgt gttcggcgtc accgtcacgc tcgccgacga ggactccggc gacgaggtcc
    11701 gttaccagat cgtcggcgag gacgaggccg acatcccgtc cgggcgcatc tcggtgaatt
    11761 ccccgatcgc gcgcgcgctg atcggcaagg aggagggcga ggtcgccacc gtgcagaccc
    11821 cgggcggcgt ccggcactgg gaagtcgtgg cggtcgagca tctgtaggcg ccggtccccg
    11881 cgcgccgctt ctctcccgac tccgaagggc aggccgatgc gcctgccctt cgcgtttcgg
    11941 ccccccgacg acgcaggttc actgccatgt accgatgcct gacccgcgcg acgctgctcg
    12001 cgatccttgc cctgagcggc ctgctgccgg ccgccgtgcc ggcggcgagc ctgcccgaca
    12061 ccatcgatgc aatccgcggc gctgtcgtcg ccgtcggcac ctacgaggcc acacgccggc
    12121 cgccggcgaa cttcctcggc accggcttcg tcgtcggcga cggcacctgg gtgatcacca
    12181 acgcccacgt cgtcgacaag ccgctcgatc cggtcaagcg cgagttcccg gccgtgttcg
    12241 ccgggcgcgg caacgagttc gcgaccatcg aggcgaagat cgtgcagacc gatcccgagc
    12301 acgacctggc gctgctgaag ctgctcggcg cgccgctgcc gacgcgcttc aggctcgccg
    12361 gcggcctgcc gcgcgagggg caggagatcg cgttcaccgg ctttccgatc ggcgtcgtgc
    12421 tcggcctgta cccggtcacg caccgcggca tcgtctcggc gatcacgccg gtcgcgatcc
    12481 cggcgccctc ggccggccag ctcgacgcca acgccatccg ccgcctgagc cggccgttca
    12541 acgtgctgca gctcgacgcc accgcctacc ccggcaacag cggcagcccg ctgtacgacc
    12601 cggcgagcgg ccgcgtctac ggcgtcatca actcggtgtt catcaaggaa accaaggaaa
    12661 acgtcatcca gaagccgagc ggcatcacct acgcgatccc ggtcgaatac ctgcaggccc
    12721 tgatgcgcaa ggccggcgcc ggcaactgac ggcttgacgc tgccggacct ggcccgtaat
    12781 atgcgcggcc tgtttggggc gattagctca gcgggagagc acttccttca cacggaaggg
    12841 gtcagtggtt cgatcccact atcgcccacc aaataaatca acggctt
//
LOCUS       DAOQIE010000072        12866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2379_length_12866_cov_7.970884, whole genome shotgun sequence.
ACCESSION   DAOQIE010000072 DAOQIE010000000
VERSION     DAOQIE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_12866_cov_7.970884"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..986)
                     /locus_tag="PK144_06345"
     CDS             complement(234..986)
                     /locus_tag="PK144_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65382.1"
                     /translation="MSDPQAPADPAGSDAGGFRAPTIFVRHARDATPFGPRPDPGRRR
                     LLGLGLLAGGCALSRVGVATTAVLQPTPALTAGPFYPRTHLPEVDADLGQLDGHARRA
                     LGEPFVLDLLVLDARGRPLPAVAVELWQANAGGRYAHPADTDASGPLDPDFQGYARLV
                     TDAEGRLRVRSVLPGAYGDGGFRRTPHLHLIFRGTGTQLTTQMFFPDQPLNAGDFIYR
                     RLSPAAQRAATARALPAGDGVRGFGWDVVLPG"
     gene            complement(1254..3329)
                     /locus_tag="PK144_06350"
     CDS             complement(1254..3329)
                     /locus_tag="PK144_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF65383.1"
                     /translation="MPNRLASEKSPYLHQHADNPVDWYPWGPEALAKAAAEDKPILLS
                     IGYSACHWCHVMAHESFEDEGVAAVMNRLFVNIKVDREERPDLDRIYQQAHQLLNGRP
                     GGWPLNVVLTPDDHMPYFSGTYFPKTPRHNLPGFPELLERLAAVWHERREDVRSQNAQ
                     LGPALRSGGPKAGVTGYAANPAPLEEVRGAILDKLDAENGGFGSAPKFPQAELFARLL
                     RIAAGARLAGTPDTQVEAAVRLALASMTTRGLYDQIGGGFFRYSVDARWEIPHFEKML
                     YDQAALLGLLGDAVAAGLLPEGGGALAASIGFLARELRAPEGGFYATLDADSEGHEGR
                     YYVWTPDEIRAALPAGDYALAAAVWGLDGAPNFEGRWHLGVRRLPVEVAAGFGLDAAA
                     VDARLDAIRARLLAVRAARVYPHRDEKILTAWNALLIGALARAGRLHGRADWIALAAD
                     AHAFLREHCWREGRLYAVCKDGEAYQPAFLDDHAYLLDATLELLQCRWQPDDLHFAQA
                     LADQLLDRFADPKKGGFHYTPVDGETLIARPKPLWDDALPGPNGVAARALLVLGRLLN
                     RIPYLVAAEKVLKWAWPGIESHPLGTTTLLGALEEYFRPGTTVVLRGTGEALQRWQQR
                     SSGRYAAQRVTIAIPDDAADLPEALALRSARADGPVAYLCTGTQCSAPITDFAAFEAE
                     LAQQEPRFG"
     gene            complement(3468..4532)
                     /locus_tag="PK144_06355"
     CDS             complement(3468..4532)
                     /locus_tag="PK144_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNF65384.1"
                     /translation="MITFTQGNLLEARVEVLVNTVNTVGVMGKGIALMFKERFAENFR
                     LYAAACKAGKVQTGRMFVTPVHELDGPRWIVNFPTKQHWRSPSRLEWIVDGLQDLRRF
                     LMEHQIHSIAIPPLGAGNGGLEWTAVREQIELALGDIATEVLVFEPTRQYQNVAKRTG
                     VEKLTPARALIAELVRRYWVMGMECSLLEIQKLAWFLERAIERHNPGNNPLNLQFTAH
                     KYGPYANRLDHLLNNLDGSYLHCNKRISDADPFEVIWFDDERKDFLQTYLKTETKAYA
                     RALESTTNLIDGFESPFGMELLATVDWLLTREGVAPDVPALREGLRHWRGGPDAAERK
                     MRLFDDRTLTIALDRLLQQA"
     gene            complement(4529..5175)
                     /locus_tag="PK144_06360"
                     /pseudo
     CDS             complement(4529..5175)
                     /locus_tag="PK144_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
     gene            complement(5565..6110)
                     /locus_tag="PK144_06365"
     CDS             complement(5565..6110)
                     /locus_tag="PK144_06365"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNF65385.1"
                     /translation="MRNVPPPPDTDATVIRRVGVPAETPAPQARVPLTGVLRGVSGEI
                     HGKVWQVYDKLLIGRDDRCDVVIPLGEVSRQHARIDGLDGKVVIHDLGSSNGTRINGN
                     MIRDAELENGDEIAIHTARLRYEIPGALPKARPAPSATPSASPIPATAAPSGGGNKTL
                     IIVAAVAVIVAIAAVAVLVLR"
     gene            6292..7500
                     /locus_tag="PK144_06370"
     CDS             6292..7500
                     /locus_tag="PK144_06370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNF65386.1"
                     /translation="MNQPVPAPHAPLRLKKNEERRLRAGHLWVYSNEVDTAVTPLTGF
                     RAGEPVEIQASNGKPVGTGYVNPQSLICARLVSRDPAHPLSGSLLVHRLKVALGLRER
                     LYPAPFYRLVHGEGDALPGLIVDRYDAVCVVQITTAGMEALKDEVIAALDKVLKPAGI
                     LLRNDTGSRKLEGLDSYVEAIGQVPEVVEILENGVRHLAPLAGGQKTGWFYDQQANRA
                     RLAKYVRGARVLDVFSYVGGWGIQAAVAGADSVMCVDSSEAALDHAHAAAELNGIAER
                     FATLEGDAFEALKNLKQERERFDVIVCDPPAFIKRKKDFKEGALAYRRINELAMSLLD
                     RDGILVSCSCSHHMERATLADTLLASARHLDRSLAFLESGQPGPDHPVHPAIPETEYL
                     KAFYSRVLPA"
     gene            7514..8308
                     /locus_tag="PK144_06375"
     CDS             7514..8308
                     /locus_tag="PK144_06375"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNF65387.1"
                     /translation="MRQYLDLLRHVREHGTDKQDRTGTGTRSVFGHQMRFDLAAGFPL
                     VTTKKVHLKSIIVELLWFLRGDTNVGFLHEHGVTIWDEWADANGDLGPVYGAQWRSWP
                     TADGRHIDQITQLLDQIRSNPDSRRLLVCAWNVGEIGRMHLPPCHCLFQFYVAGGRLS
                     CQLYQRSADLFLGVPFNIASYALLTQMLAQVCGLEAGEFIWTGGDCHIYANHFEQVDT
                     QLAREPLPLARMVLNPEVRDLFAFRYEDFRLEGYESHPAIKAPVAV"
     gene            complement(8316..9668)
                     /locus_tag="PK144_06380"
     CDS             complement(8316..9668)
                     /locus_tag="PK144_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF65388.1"
                     /translation="MPRIRSFARTTAFRLSLLFALLYGSCVAVLLGGVSWSILRYVDG
                     AVRDELNAELDVYRAIYRDLGADGLRSALKRVHDGRSVTGRTLALLDGEGRLVLGSLP
                     VELPAGLERNSVQSVAGNEYLLITGSLADGSLLAIAQGPDGIRGLHRYIQELARYVLP
                     LGLLIAVLLGLVLGKRNSRRVETIARTADEIMAGDLTRRLEISNHHDEIDAVALRLNR
                     ILDRLEALMSGMRRFSDNVAHDLRTPLTRMRNRVEVMLLEERTPSEYRTVLVQTSDEA
                     ERLIGTFNALLSISRAEAGVQSDHWAPLELGPLACELAEFYDALAEERELTLRCEAGD
                     DDCPPVIGDRHLLAQAIGNLLDNALKFTPRGGSVALSVHMRFGQPTLEVADTGPGIPP
                     KERQRVLERFERLEASRDVPGNGLGLALVRAVAQLHGAQLELDDNRPGLIVRLRFAAE
                     "
     gene            complement(9652..10326)
                     /locus_tag="PK144_06385"
     CDS             complement(9652..10326)
                     /locus_tag="PK144_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF65389.1"
                     /translation="MNILVIEDDTVAAAYLVKGLNESGYRVEHAVRGDDGLVRALEGR
                     HDALIVDRMLPGLDGLSLVSRLRESGSTVPILVLSALAELDDRVVGLRAGGDDYLTKP
                     YAFAELLARLEALTRRHGTAPPVRLRVADLELDLLAHTAVRAGAPVRLQPREFRLLEY
                     LARHAGQVVTRAMLLEHVWAYSFDPQTNVIDVHISRLRAKIDRAGLPPLIHTLRGIGY
                     KLAENP"
     gene            10507..12036
                     /locus_tag="PK144_06390"
     CDS             10507..12036
                     /locus_tag="PK144_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNF65390.1"
                     /translation="MSAPPPHRNTVRSLDPYRHRGGPLALPLAPLKASLHLFGLMLTM
                     FSVTMLPPMAIGVLYGDGAVEPFAHTLLITLASGLLIWLPVRRQKLDLRNRDGFLVVV
                     AFWVTLSLLSAIPFMLSEQPHMPLVDAVFEAVSGLTTTGATVLAGIDSLPHAIVYYRA
                     QLNFFGGMGIVVLAVALLPMLGVGGMQIYRAETPGPMKDEKLTPRITETAKNLWYIYV
                     GLNVLCTAAFWLAGMDLFDSVCHSFATIALGGFSTHDASIGFYQSDAIEFVAGFFSLV
                     AAVNFALFFFAWRQKSLRAILVDEEFRFFVKVMAVLIGAAIAGLYFSGTFPLWESIVH
                     GFFQTASIVTDNGLVAAGYPDWPMAVTLLLLLGSFFGGCVGSTCGGIKAIRFLLLYRQ
                     SVREMKLLIRPTAQIPVRLGTRTVPDRVMQAVLAFYFLYIFAYCACSIGVAATGADLV
                     TAFGSVAGCINNMGVGLGETASNFSPLSDTATWIVTASMLIGRLEVFPLLLLFVPSFW
                     R"
     gene            12201..12758
                     /locus_tag="PK144_06395"
     CDS             12201..12758
                     /locus_tag="PK144_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65391.1"
                     /translation="MLLWLAAAPAQATAVIEDPAAGGHSVFVWEAAGALSGIFDAGPD
                     DGVSSEAGSTDWQLALAAPGLLGIVISGVYNRPPTVLDLLIDGIGVPWTTSSVRAEVI
                     SEDDQGVPLEIINHFDARLDGLLLAAGEHRLTLLPSADNTDGTAGFITFAAVVPVPAP
                     PALALLAAGFGLVVALRARRTAKAA"
BASE COUNT         2016 a         4412 c         4377 g         2061 t
ORIGIN      
        1 gccgcagccg gaactgcgcc tgacgcgctc gcccttcgtc gccgactaca cgctctgcga
       61 cgccgccggc tgcacggcac tcggggcgcg gctgccggcc ggggccgggg aagtggtgcg
      121 gttgcagccg tgcccgggcg ctgcggataa cggcggcggc tgaagcgcag cgccccggcc
      181 gccgcgacaa gcccccgagg cccggcccgc ccgcagtgcg cggcaacccc cactcagccc
      241 ggcagcacca catcccagcc gaagccgcgc acgccgtcgc cggccggcag tgcgcgggcg
      301 gtggcggcgc gctgtgcggc cgggctcagg cggcggtaga tgaaatcccc ggcattcagc
      361 ggctggtccg ggaagaacat ctgcgtggtc agctgcgtgc cggtgccgcg gaagatcagg
      421 tgcaggtgcg gcgtgcgccg gaaaccgccg tcgccgtagg cccccggcag caccgagcgt
      481 acgcgcagcc ggccttcggc atcggtcacg agtcgcgcat agccctggaa atccggatcg
      541 agcgggccgc tggcgtcggt atcggccggg tgcgcatagc gaccgccggc gttggcttgc
      601 cacagctcga ccgccaccgc cggcagcggc cggccgcggg cgtcgagcac cagcagatcg
      661 agcacgaagg gctcgccgag cgcgcgccgg gcatggccgt cgagctggcc gagatcggca
      721 tcgacttcgg gcaggtgcgt gcgcggatag aacggtccgg cggtcagcgc cggcgtcggc
      781 tgcagcaccg ctgtcgtcgc cacgccgacc cgcgacagcg cacagccgcc ggcgagcagt
      841 ccgagcccga gcaggcgccg gcggccggga tcggggcgcg ggccgaaggg cgtggcgtca
      901 cgggcgtggc gcacgaagat cgtcggcgca cggaaaccgc cggcatcgga cccggccgga
      961 tcggccggag cttgcggatc ggacatcgga cacctcgcct gcggaccccg cggtccgcgc
     1021 cgggccggcc ggcaggggcc ggccgttcag cggtgcggcg catcgcttcc gggccggtag
     1081 accgccgcgg cccttcttcg tcgcgcccgg gatcgcaaca tagcgcgcgc cgacgcatgc
     1141 cgcgcaccgg cgggcgtctc cgcagttctc gcgttcccga tatccgccgc acgcccttca
     1201 tcgccgaagc aagactgacc cggcccccgg aacccgacca ccaagcagcc cgctcacccg
     1261 aagcgcggct cctgctgcgc cagctccgcc tcgaaggccg cgaagtccgt gatcggggcg
     1321 ctgcactggg tgccggtgca gagatacgcg accgggccgt cggcgcgggc gctgcgcagg
     1381 gccagcgcct cgggcaggtc ggcggcgtcg tccggaatcg cgatcgtcac gcgctgcgcg
     1441 gcgtagcggc cgctgctgcg ctgctgccag cgttgcagcg cctcgcccgt gccgcgcagc
     1501 acgaccgtcg tgccggggcg gaagtattcc tccagcgccc cgagcagcgt cgtcgtgccg
     1561 agcgggtggc tttcgatgcc cggccaggcc cacttcagca ccttctcggc cgcgaccaga
     1621 tacgggatgc ggttcagcag ccgcccgagc acgagcagtg cgcgcgcggc aacgccgttg
     1681 ggcccgggca gcgcgtcgtc ccacagcggt ttcggccgcg cgatcagggt ttcgccatcg
     1741 accggcgtgt agtggaagcc gcccttcttc ggatcggcga agcggtcgag cagctgatcg
     1801 gcgagcgcct gcgcaaagtg cagatcatcc ggctgccagc ggcactgcag cagttcgagc
     1861 gtcgcatcga gcaggtaggc gtgatcgtcg aggaaggccg gctgatacgc ctcgccgtcc
     1921 ttgcagaccg cgtacagccg gccctcgcgc cagcagtgtt cgcgcaggaa ggcgtgtgca
     1981 tcggcggcga gcgcgatcca gtccgcccgc ccgtgcaggc ggccggcacg cgcgagcgcg
     2041 ccgatcagca gcgcattcca ggccgtcagg atcttctcgt cgcgatgcgg gtagacgcgc
     2101 gccgcacgca cggcgagcag gcgggcgcgg atggcatcga ggcgcgcatc gaccgcagcc
     2161 gcatcgagcc cgaagccggc cgcgacctcg accggcagcc ggcgcacgcc gaggtgccag
     2221 cggccttcga agttcggcgc cccgtcgagc ccccagacgg cagcggcgag cgcgtagtcg
     2281 ccggccggca gcgcggcgcg gatttcgtcc ggtgtccaca cgtagtaacg gccttcgtgg
     2341 ccttcggaat cggcgtcgag cgtcgcgtag aagccgcctt cgggggcgcg cagttcgcgg
     2401 gcgaggaatc cgatgctggc ggccagcgcg ccgccgcctt cgggcagcag gccggcggcc
     2461 acggcatcgc cgagcaggcc gagcagcgcg gcctggtcgt agagcatctt ctcgaagtgc
     2521 ggaatctccc agcgcgcatc gacgctgtag cggaagaagc cgccgccgat ctggtcgtac
     2581 agcccgcgcg tcgtcatcga cgcgagcgcc agacgcacgg cggcctcgac ctgcgtgtcg
     2641 ggtgtgccgg cgagccgggc gccggcggcg atgcgcagca gccgcgcgaa cagctcggcc
     2701 tgcgggaatt tcggcgcgct gccgaagccg ccgttctccg catccagctt gtcgaggatc
     2761 gcgccgcgca cctcttcaag tggcgccgga ttggcggcat agccggtgac accagccttc
     2821 ggcccgccac tgcgcagcgc cggcccgagt tgcgcattct gcgaacgcac gtcctcacgc
     2881 cgctcgtgcc agaccgcggc cagccgttcg agcagttccg gaaagcccgg caggttgtgg
     2941 cgcggcgtct tcggaaaata cgtgccgctg aaatacggca tgtgatcgtc gggcgtcagc
     3001 accacgttca acggccagcc gcccggccgg ccgttcagca actggtgcgc ctgctggtaa
     3061 atccggtcga gatcgggccg ctcctcgcga tcgaccttga tgttgacgaa cagccggttc
     3121 atgaccgcgg ccacgccttc gtcctcgaag gattcgtgcg ccatcacatg gcaccagtga
     3181 cacgccgaat aaccgatcga caacaggatc ggcttgtcct cggccgcggc tttcgcaagc
     3241 gcttcgggcc cccacggata ccagtcgacg gggttgtcgg cgtgctggtg gaggtagggg
     3301 gatttttcgg aggcgaggcg gttgggcatg gggggagcct gcagagtgat tggccttaca
     3361 agactgatga tgttatgccg atcacgcggg aacgctctgc ttttcagccc ccggcctttc
     3421 tggggggaaa aacaatctat tcccgtggga tcggcaggat tgcgcattca cgcctgctgc
     3481 agcagacggt cgagggcgat tgtcagtgtc cggtcgtcga acaggcgcat cttgcgctcg
     3541 gccgcatccg ggccgccgcg ccagtggcgc aagccttccc gcagagccgg cacatcggga
     3601 gccaccccct cgcgcgtgag caaccagtca accgttgcca gcaattccat gccgaacggc
     3661 gattcgaaac catcgatcag attcgtggtt gattccagtg cccgggcgta cgctttggtt
     3721 tctgtcttca aataggtttg caggaaatcc ttgcgctcgt cgtcgaacca gatcacctcg
     3781 aacgggtcgg catcgctgat acgcttgttg caatgcaggt agctaccatc gaggttgttc
     3841 agcaggtggt ccagccggtt cgcgtaaggg ccgtatttgt gggccgtgaa ctgcaggttc
     3901 agcgggttgt tgccgggatt gtggcgctcg atggcccgtt ccagaaacca cgccagtttc
     3961 tggatttcca gcagactgca ttccatgccc ataacccagt aacggcgcac cagttcggca
     4021 atcagggcgc gcgccggcgt cagtttttcc acgccggtcc gcttggccac gttctgatac
     4081 tggcgggtgg gctcgaacac caggacttcg gttgcgatgt cgcccagggc cagttctatc
     4141 tgctcacgca ccgcagtcca ttccagacca ccattaccgg cccccagtgg cgggatggcg
     4201 atcgaatgaa tctgatgctc catcaggaac cgccgcaaat cctgcaggcc atcgacgatc
     4261 cactccagac gggaaggcga acgccagtgc tgcttggtcg gaaagttgac gatccagcgc
     4321 ggaccgtcca gttcatgcac cggcgtcacg aacatcctgc cggtctgcac tttcccggcc
     4381 ttgcaggcag cggcgtacag gcggaagttc tcggcaaagc gctccttgaa catcagcgca
     4441 atgcccttgc ccatcacgcc gacggtgttg acggtgttga ccagcacctc gacacgggct
     4501 tccagcagat tgccttgagt gaatgtaatc atccgaaata ccactcgggc cgggcatgaa
     4561 ctggcagatt caggtttcgg gcttgaactt cctgagcaat aaggattttc aatgcctcag
     4621 tataacacac cattccaagc aaaccgctca ccggcaagga tggtagatca gggcctcagc
     4681 ctgataacgc tcaaatttgg ccggatcatc cggatcacac ctgaaatctc gccgctgcag
     4741 caggggccag tctatattgg tcagatcggc caggtcagaa taaaaatttg cccaccgata
     4801 gtaggcatga ctatccgtga acagaaacgg caaaccctgt ccagcgacat ggtgcaggct
     4861 ggacaccagg atcacgacat cctcattagt ccgcttctgg atgccgcccc aacccgtgcg
     4921 aatgttgtgc aacattggcg aaaaaggggt gaagtagaag ggtatataat cgttcagcac
     4981 accgcccggc gcctgcggca ccggatggct ggcgcgcttg ctgatcagtt ccgggttacc
     5041 gatgttgatc cagtccggcg ccttcaccgg actgttgccg cagtgcagac cattagccag
     5101 aatccacggc aggttgtcgc ggtgaacaat gcgccagatc agcgcctttt ccggattgag
     5161 atttctgtaa ctcatcatcg gcaaccatga tgcggatgaa gccgaaaggt gcgggattca
     5221 gggtgcagaa gtgcagggaa gcgcctgacg cagccggagt gtctgtcttt cgtgtttcgt
     5281 ttccgaatcg ggccggcagg actgtgcgag tcacggcctg acccgcagtc gatgtacaga
     5341 tcgtgccggt gccgctacgg cgtggcgctg cggaccgaga cgctgctgcc acgcctgaga
     5401 aacgggcttg cgaagccggc ccgccggtcc gggcatcatc aggaagcctg gcctcaccgc
     5461 gccgtgtcca tgctgctctc ctgcgggcag caggcttcag ggtaagcgca accatcgccg
     5521 ccgatccgcc gcgccgtacc ggcgcagcgg atcgacatcc ggactcagcg cagcaccagc
     5581 accgccaccg ccgcgatcgc gacgatgacg gcgaccgctg caacgatgat cagcgtcttg
     5641 ttgccgccgc cgctcggggc cgcggtggcc gggatcgggc tggcggacgg cgtggcgctc
     5701 ggggccgggc gggccttggg cagggcgccg gggatttcgt agcgcaggcg ggcggtgtgg
     5761 atggcgatct cgtcgccgtt ttcgagttcg gcgtcgcgga tcatgttgcc gttgatgcgc
     5821 gtgccgttcg aggagccgag gtcgtggatc acgaccttgc cgtcgaggcc gtcgatgcgc
     5881 gcgtgctggc gcgagacctc gccgagcggg atcacgacat cgcagcggtc atcacggccg
     5941 atcagcagct tgtcgtagac ctgccagacc ttgccgtgaa tctcgccgct gacgccgcgc
     6001 agcacgccgg tcagcggtac gcgggcctgc ggggcagggg tttcggcggg gacaccgaca
     6061 cggcggatga cggtggcatc ggtatcgggc ggcggcggga cgttacgcat cgggttcctc
     6121 cagagttcgg cacggccggg ctgcaagcgg ttggctacgg caaaggtagc gacggaaacg
     6181 ggcgagcggc ggggacgcac acgcgtatcg cagtttacct tgtccggaca gcacgctagc
     6241 cttccggact ttttcccgcg cgccacccgc gcgccgccag gtaccgaccc gatgaaccag
     6301 cctgtccccg ccccccacgc cccgctgcgc ctgaagaaga acgaggaacg gcgcctgcgg
     6361 gccggccacc tgtgggtgta cagcaacgag gtcgataccg ccgtcacgcc gctgaccggc
     6421 ttccgggccg gcgagccggt cgagattcag gccagcaacg gcaagccggt cggcaccggc
     6481 tacgtcaatc cgcagtcgct gatctgcgcg cggctggtca gccgcgatcc ggcgcacccg
     6541 ctgtcgggct cgctgctcgt gcaccggctg aaggtggcgc tgggcttgcg cgagcggctg
     6601 tatccggcgc cgttctaccg gctcgtgcac ggcgagggcg acgcgctgcc ggggctgatc
     6661 gtcgatcgct acgacgcggt ctgcgtcgtg cagatcacga ccgccggcat ggaggcgctg
     6721 aaggatgagg tcatcgccgc gctcgacaag gtgctgaagc ccgccggcat cctgctgcgc
     6781 aacgacaccg gcagccgcaa gctcgaaggg ctggacagct acgtcgaggc catcggtcag
     6841 gtgccggagg tggtcgagat cctcgaaaac ggcgtgcgcc atctggcgcc gctcgccggc
     6901 ggccagaaga ccggctggtt ctacgaccag caggccaacc gcgcgcgcct cgccaagtac
     6961 gtgcgcggcg cgcgcgtgct cgacgtgttc agctacgtcg gcggctgggg catccaggcg
     7021 gcggtggcgg gggccgacag cgtcatgtgc gtcgacagct ccgaagcggc gcttgaccac
     7081 gcgcacgcgg cggccgaact caacggcatc gccgagcgct tcgcgacgct cgaaggcgat
     7141 gccttcgagg cgctgaagaa cctgaagcag gagcgcgagc gcttcgacgt gatcgtctgc
     7201 gacccgccgg ccttcatcaa gcgcaagaag gacttcaagg aaggcgcgct cgcgtaccgg
     7261 cgcatcaacg aactggcgat gagcctgctc gatcgcgacg gcatcctggt ctcgtgctcg
     7321 tgctcgcatc acatggagcg cgcgacgctc gccgacacgc tgctcgcgag cgcccgccac
     7381 ctcgaccgct cgctcgcctt cctcgaatcc ggccagccgg gcccggatca cccggtgcat
     7441 ccggcgatcc cggagaccga atacctgaag gccttctaca gccgcgtgct gccggcctga
     7501 ggctcgaccc gcaatgcgcc agtacctcga cctgctgcgc cacgtgcgcg agcacggcac
     7561 cgacaagcag gaccgcaccg gcaccggcac gcgctcggtc ttcggccatc agatgcgctt
     7621 cgacctcgcc gccggctttc cgctggtgac gacgaaaaag gtgcatctga agtcgatcat
     7681 cgtcgaactg ctgtggttcc tgcgcggtga caccaacgtc ggcttcctgc acgagcacgg
     7741 cgtgacgatc tgggacgaat gggccgatgc caatggcgat ctcgggccgg tgtacggcgc
     7801 gcagtggcgc agctggccga cggccgatgg ccgccacatc gaccagatca cgcagctgct
     7861 cgatcaaatc cgcagcaacc cggattcgcg gcggctgctg gtctgcgcgt ggaatgtcgg
     7921 cgagatcggg cgcatgcacc tgccgccgtg ccactgcctg ttccagttct acgtcgccgg
     7981 cgggcggctg tcctgccagc tctaccagcg ctcggcggac ctgttcctcg gcgtgccgtt
     8041 caacatcgcg agctacgcgc tgctgacgca gatgctcgcg caggtctgcg gcctcgaagc
     8101 cggcgagttc atctggaccg gcggcgactg ccacatctat gcgaaccact tcgaacaggt
     8161 cgacacccag ctcgcgcgcg agccgctgcc gctcgcgcgc atggtgctga acccggaggt
     8221 ccgcgacctg ttcgcgttcc gctacgagga tttccggctc gaaggctacg agagccatcc
     8281 ggcgatcaag gcaccggtgg cggtgtagct gcgcttcact cggccgcgaa gcgcagccgc
     8341 acgatcagcc cgggccggtt gtcgtcgagt tcgagctgcg cgccgtgcag ctgcgcgacc
     8401 gcgcgcacca gcgcgaggcc cagaccgttg ccgggcacgt cgcgcgaggc ttcgaggcgc
     8461 tcgaagcgct ccagcacgcg ctggcgctcc ttcggcggaa tgccggggcc ggtatcggcg
     8521 acctccagcg tcggctggcc gaagcgcatg tgcaccgaca gcgcgacgct gccgccacgc
     8581 ggcgtgaact tcagcgcgtt gtcgagcagg ttgccgatcg cctgcgcgag caggtggcga
     8641 tcgccgatca ccggcggaca gtcgtcgtca ccggcctcgc agcgcagcgt cagctcgcgt
     8701 tcctcggcga gcgcgtcgta gaactcggcc agttcgcagg ccagcggtcc gagttcgagc
     8761 ggcgcccagt gatcggactg cacgccggcc tcggcgcgcg agatgctgag cagcgcgttg
     8821 aaggtgccga tcaggcgctc ggcctcgtcc gaggtctgca cgagcacggt gcggtactcc
     8881 gagggcgtgc gctcttcgag cagcatgacc tcgacgcggt tgcgcatgcg cgtcagcggc
     8941 gtgcgcaggt cgtgcgcgac gttgtccgag aagcggcgca tgcccgacat cagcgcttcc
     9001 agccggtcga ggatgcggtt caggcgcagc gccaccgcgt cgatctcgtc gtggtggttg
     9061 ctgatctcca gccgccgggt caggtcgccg gccatgatct cgtcggcggt gcgcgcgatc
     9121 gtctcgacgc ggcggctgtt gcgcttgccg agcaccagcc cgagcagcac ggcgatcagc
     9181 aggccgagcg gcagcacgta gcgcgcgagt tcctggatgt agcggtgcag gccgcggatg
     9241 ccgtccggcc cctgcgcgat cgccagcagc gaaccatcgg cgagcgagcc ggtgatcagc
     9301 aggtattcgt tgccggccac cgactgcacg ctgttgcgtt cgagcccggc cggcagctcg
     9361 accggcagcg agccgagcac cagccggcct tcgccatcga gcagcgccag ggtccggccg
     9421 gtgacgctgc gcccgtcatg cacgcgcttc agcgccgagc gcaggccatc ggctccgaga
     9481 tcgcggtaga tggcgcgata gacatcgagc tcggcattga gctcgtcgcg caccgcgcca
     9541 tcgacgtagc gcaggatcga ccatgacacg ccgccgagca gcacggcgac gcaactgccg
     9601 tagagcagcg cgaacagcag cgacagccgg aaggccgtcg tgcgcgcgaa gctacggatt
     9661 ctcggcaagt ttgtagccga tgccgcgcag cgtgtggatc agcggcggca ggccggcgcg
     9721 atcgatcttg gcgcgcaacc ggctgatgtg cacgtcgatg acgttggtct gcggatcgaa
     9781 gctgtaggcc cacacgtgtt cgagcagcat cgcgcgcgtg accacctgtc cggcgtggcg
     9841 cgcgagatat tcgagcagcc ggaactcgcg cggctgcagg cgcaccggcg caccggcgcg
     9901 cacggccgtg tgggcgagca ggtcgagttc gaggtcggcg acgcgcaggc gcaccggcgg
     9961 cgcggtgccg tgccgccggg tcagcgcttc gagccgcgcg agcagctcgg cgaaggcgta
    10021 gggcttggtc aggtagtcgt ccccgccggc acgcaggccg acgacgcggt cgtcgagctc
    10081 ggccagtgcc gacagcacca ggatcggcac cgtcgagccg gattcgcgca gccggctgac
    10141 cagactcagt ccgtcgagcc cgggcagcat gcggtcgacg atcagcgcgt cgtgccgacc
    10201 ttcgagcgcc cgcaccagac catcgtcgcc gcgcacggcg tgctcgacgc gataaccgct
    10261 ctcgttgagt cctttgacca gatacgccgc agctacggtg tcgtcttcga tgacgaggat
    10321 gttcataggg gctcgaagct cgctggaaac ggcaggcagg aaaggtaacg gcccggcatg
    10381 taaatttagt tgaaaaattg cgttttggta atgctttatg tgcagccggc caacgacgac
    10441 gacgatcgtc gcagccgatt cccgatcccc tgccagacgc cggcccgcct gccggccggg
    10501 agcccgatga gcgccccgcc tccgcaccgc aacaccgtcc gctcgctcga tccctaccgc
    10561 caccgcggcg ggccgctggc gctgccgctg gcgccgctga aggccagcct gcacctgttc
    10621 ggactgatgc tgacgatgtt cagcgtcacg atgctgccgc cgatggcgat cggcgtgctc
    10681 tacggcgacg gcgcggtcga gccgttcgca cacacgctgc tgatcacgct cgcgagcggc
    10741 ctgctgatct ggctgccggt gcgtcgccag aagctcgacc tgcgcaaccg cgacggcttt
    10801 ctggtcgtcg tcgcgttctg ggtgacgctg tcgctgctgt cggcgatccc gttcatgctc
    10861 tccgagcagc cgcacatgcc gctggtcgat gcggtgttcg aggccgtctc ggggctgacc
    10921 acgaccggcg ccaccgtgct cgccggcatc gacagcctgc cgcacgcgat cgtgtattac
    10981 cgcgctcagc tcaacttctt cggcggcatg ggcatcgtcg tgctggcggt ggcgctgctg
    11041 ccgatgctcg gggtcggcgg catgcagatc taccgcgccg agacgcccgg cccgatgaag
    11101 gacgaaaagc tcacgccgcg catcaccgag accgccaaga acctctggta catctacgtc
    11161 ggcctgaacg tgctctgcac cgccgcgttc tggctggccg gcatggacct cttcgattcg
    11221 gtctgccaca gcttcgcgac catcgcgctc ggcggcttct cgacgcacga cgcctcgatc
    11281 ggcttctatc agagcgacgc catcgagttc gtcgccggct tcttctcgct ggtcgcggcc
    11341 gtcaacttcg cgctgttctt cttcgcgtgg cgccagaaga gcctgcgcgc gatcctcgtc
    11401 gacgaggagt tccgcttctt cgtcaaggtc atggccgtgc tgatcggcgc ggcgatcgcc
    11461 gggctgtact tcagcggcac cttcccgctg tgggagtcga tcgtgcacgg cttcttccag
    11521 accgcctcga tcgtcaccga caacggcctg gtcgccgccg gctatccgga ctggccgatg
    11581 gcggtgacgc tgctgctgct gctcggcagc ttcttcggcg gctgcgtcgg ctcgacctgc
    11641 ggcggcatca aggcgatccg cttcctgctg ctgtaccgcc agagcgtgcg cgagatgaag
    11701 ctgctgatcc gcccgaccgc gcagatcccg gtgcgcctcg gcacgcgcac ggtgccggat
    11761 cgcgtcatgc aggcggtgct ggcgttctac ttcctgtaca tcttcgcgta ctgcgcgtgt
    11821 tcgatcggcg tcgccgcaac cggcgccgac ctcgtcaccg ccttcggctc ggtcgccggc
    11881 tgcatcaaca acatgggcgt cggcctcggc gagacggcct cgaacttctc gccgctcagc
    11941 gacaccgcga cctggatcgt caccgcctcg atgctgatcg gccggctgga ggtgtttccg
    12001 ctgctgctgc tgttcgtgcc gtcgttctgg cgctgagcgg gcgttgccgg aaataccctt
    12061 cctccggaca agaccgcctg attccggccg ccctccatcg cgcgggtcca cgccggctac
    12121 actcaccggc cacgacgggc accggagatg gccggaggtg cgacatgctg cagcgtcttt
    12181 tcggcgtccc ggtactgagc gtgctgctgt ggctcgcggc cgctccggcg caggcgacgg
    12241 ctgtgatcga ggatcccgcc gccggcggtc acagtgtgtt tgtctgggaa gcagccggtg
    12301 ccctctcggg catcttcgat gcgggtccgg acgacggcgt ctccagcgaa gccggcagca
    12361 ccgactggca gctcgccctt gcggcgccgg gcctgctcgg gatcgtcatc agcggcgtct
    12421 acaaccgtcc gccgacggtg ctcgatctgc tgatcgacgg catcggcgta ccctggacca
    12481 cctccagcgt ccgcgccgag gtgatcagcg aggatgatca gggcgtaccg ctggagatca
    12541 tcaaccactt cgatgcccgg ctcgatggcc tgctgctggc ggccggggaa caccggctga
    12601 cgctgctgcc ctcggccgac aacaccgacg gcacggcagg tttcatcacc tttgccgccg
    12661 tcgtgccggt accggctccc ccggcactgg ccctgctggc ggccggtttc gggcttgtcg
    12721 tggccttgcg ggcacgtcgg accgccaaag cagcctgaag cgcaggggca tatcccttcc
    12781 ctgcgcaggc ggtaacgacg cccgggcagc gcggccgtcg ccgcggcgcg aagccccggc
    12841 ggcctcaccc gaagccgcgc agcgcc
//
LOCUS       DAOQIE010000073        12823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2386_length_12823_cov_7.118813, whole genome shotgun sequence.
ACCESSION   DAOQIE010000073 DAOQIE010000000
VERSION     DAOQIE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12823
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_12823_cov_7.118813"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..559)
                     /gene="acpS"
                     /locus_tag="PK144_06400"
     CDS             complement(152..559)
                     /gene="acpS"
                     /locus_tag="PK144_06400"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNF65392.1"
                     /translation="MILGLGTDIVRVARMEEIIARHGERFAEKVLTADELDEYRASRH
                     PARLLAKRFAVKEAALKALGTGVQQGLTLAQVGLGHDALGCPHLILSGAAAARLQALG
                     ASATHVSISDEREYALAVVIVEGDAGPPGRAPA"
     gene            complement(556..1314)
                     /gene="pdxJ"
                     /locus_tag="PK144_06405"
     CDS             complement(556..1314)
                     /gene="pdxJ"
                     /locus_tag="PK144_06405"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNF65393.1"
                     /translation="MNAPTPLLLGVNIDHVATLRQARGSTRYPEPVQAALVAEQAGAD
                     AITLHLREDRRHIQDRDVELIAQVLQTRMNFEMAVTAPMLALAARLRPADVCLVPERR
                     QELTTEGGLDVAGGFPRVREACERLAEAGCAVSLFIDADPEQIDAAARSGAPKIELHT
                     GRYAEAATADELATELARLRAAAAQAHALGLVVNAGHGLHYHNVAAIAAIPELHELNI
                     GHAIVAQALFDGLAQAVRDMKRRMLEARARVAGR"
     gene            complement(1311..2066)
                     /gene="recO"
                     /locus_tag="PK144_06410"
     CDS             complement(1311..2066)
                     /gene="recO"
                     /locus_tag="PK144_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNF65394.1"
                     /translation="MSPGLEPAWVLHTRPYRDTSLLLELLTLTRGRVGAVARGARGPR
                     SRSRGWLRPFQPLLVGLAGRGELATLTSAEAAGAAFWLAGERAVAGLYLNELLLKLLA
                     RDDPQPLLLGHYRAALVALAEPADPEPALRVFECELLTALGYAPALAVDTDGEPLQAD
                     ARYHYRPDAGARRLPGLAEAPAGGVELSGRTLLALAAGAESFTCPDRDTLREARTLTR
                     SLIDHCLGGRPVRTRELARAFRSLAAPAGGKPA"
     gene            complement(2085..3032)
                     /gene="era"
                     /locus_tag="PK144_06415"
     CDS             complement(2085..3032)
                     /gene="era"
                     /locus_tag="PK144_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNF65395.1"
                     /translation="MTEPTEPTATPDSDADAPNTRAGYVALLGRPNVGKSTLLNRLLA
                     FKLSITSPKPQTTRHTILGIQTLADTQIVYVDTPGLHLNGRKAMNRYMNRAAGNVLGY
                     VDAVVFVIEALRWTDEDAAVLERLADYAGPVLLAVNKVDRVTDKPKLLPFLQQMAEKR
                     AFAAIVPLSAQKGTNAAALETEIAKHLPANPFFFDEDQITTASKRFLAAELVREKLTR
                     VLSDELPYALTVEIEKFEVEGRLHRISAVIWVERKGQKAIVIGDKGGTLKEAGRLARL
                     DMEEMFDAKVHLETWVKVREGWSDDEKALRSLGFDESED"
     gene            complement(3090..3782)
                     /gene="rnc"
                     /locus_tag="PK144_06420"
     CDS             complement(3090..3782)
                     /gene="rnc"
                     /locus_tag="PK144_06420"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNF65396.1"
                     /translation="MHTGLPSAAARLEPALGHRFRDPALRDAALTHRSAGGSNNERLE
                     FLGDALLGCVIGEALYQRLPRAPEGDLTRLRALLVREATLAELARELALGDALALGGS
                     ELKSGGYRRASILADAFEALIGAIYLDSDFETCRRVVLALFGPRLDALPSPSELKDPK
                     TRLQEYLQARQQPLPAYRIVEVSGEAHAQTFTVECTCSGLPAVVASGTSRRKAEQEAA
                     GRMLATLTAGGH"
     gene            complement(3796..4158)
                     /locus_tag="PK144_06425"
     CDS             complement(3796..4158)
                     /locus_tag="PK144_06425"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNF65397.1"
                     /translation="MSPITVLLVCGVIGFCMLMVFRIAPIYIADATVSSAMKGIEQEA
                     NLARMSAYEIRNRLQRHFDVNDIDAIKSSDVKVTKDAGELVISIEYETRRPLIYNLDV
                     VAHFTHEQRVKIASASGL"
     gene            complement(4212..4991)
                     /gene="lepB"
                     /locus_tag="PK144_06430"
     CDS             complement(4212..4991)
                     /gene="lepB"
                     /locus_tag="PK144_06430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNF65398.1"
                     /translation="MTFDFAAALVLAALVTGLVYALDVWKLAPARAAAGGVGGPRPMP
                     WYVDWSRSFFPVIVVVLLLRSFIVEPFRIPSGSMLPTLRAGDFILVSKFSWGLRLPVS
                     NTEILSTGEVKRGDVAVFRYPEDPSTAFIKRIVGLPGDRLVYRDKQLFVNGQAVEQTK
                     LPGPVSDLEPGFEARQEQLGDVKHLIQVAPRMLDSGAREYVVPPGHYFAMGDNRDNSR
                     DSRYWGYLPAENLIGKAFMIWMNWDCVTGNGHCGRIGRRIE"
     gene            complement(5154..6959)
                     /gene="lepA"
                     /locus_tag="PK144_06435"
     CDS             complement(5154..6959)
                     /gene="lepA"
                     /locus_tag="PK144_06435"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNF65399.1"
                     /translation="MASMDHIRNFSIIAHIDHGKSTLSDRIIQICGGLSEREMSAQVL
                     DSMDLERERGITIKAQSVSLDYTARNGETYQLNFIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDAAQGVEAQSVANCYTAIEQGLEVLPVLNKIDLPQCEPERVIDEIGDIIGIDASD
                     AVRVSAKTGLNVVDLLEQLVERIPAPKGDPDAPLKALIIDSWFDAYVGVVSLVRVVDG
                     RIAPRQKIRMMSSDRVYQVDKVGVFTPKRFDKPVLAAGEVGFVIGNIKEIDGAPVGDT
                     ITDAQNPAAAALPGFQKVQPRVFAGLFPIESDQYEDLREALAKLRLNDASLHYEPETS
                     QALGFGFRCGFLGMLHMEIIQERLEREYDLDLITTAPTVVYEVLTTAGEIAYIDNPDK
                     LPASNYLDEIREPIILANILVPADYLGNVITLCIEKRGVQRNLHYAAGGQVQLSFELP
                     MSEVVLDFFDRLKSVSRGFASFDYSFERFQAEPLVKLDVLINGEKVDALAIIVHREQA
                     PYRGRDLVEKMKDLIPRQMFEVAIQAAIGSHIIARSTVKALRKNVLAKCYGGDVSRKR
                     KLLEKQKEGKKRMKSVGKVDIPQEAFLAVLQVDKK"
     gene            complement(7032..8393)
                     /locus_tag="PK144_06440"
     CDS             complement(7032..8393)
                     /locus_tag="PK144_06440"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNF65400.1"
                     /translation="MVVWRGALAGVLWLLGCAGALAAGLPDFPALVKRNAGSVVNISS
                     IGKLAAATEPAADDNSDDGDEDASPDAADALGTGLVIAADGWLLTNRHVIADAEDIVV
                     RLPDRRELPARVIGTDERTDLALLKIDADGLQPATVGRADALEVGEWVLAIGSPFGFE
                     QSVTAGIVSAKGRSLPSEPYVAYIQTDVPINPGNSGGPLINLRGEVIGINSQIYSQTG
                     GYMGLSFAIPIETAMDVARQLRDSGHVSRGWLGVLVQPLTRDLAESFGLASPRGALVS
                     RVFPDGPAAAAGLRTGDVITRFDSHEIDLSTALPPLVGATGVDRRVELAIMRGGKPQT
                     LAVQIAALPDNEPPVASTEPVVTSERPLDRLGLRVAPVPPELRRQLGIPEGGVLVEQV
                     DNSRAARAGLRQGDVLGMLNGAQIRDPDAFDRTLEDLPAGRAIALLVLRRSGPAYLAL
                     RVP"
     gene            complement(8532..8957)
                     /locus_tag="PK144_06445"
     CDS             complement(8532..8957)
                     /locus_tag="PK144_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNF65401.1"
                     /translation="MIVEEGIVLGPAADGRLLVETRRRSACGSCQAQAGCGTAAIGKV
                     TGGRRNVVAALADADYRSGEHVELGLAEGALLAGSAILYLVPLLGLLGGALAGSPFGQ
                     GTSAASGMAGLLLALLWTRRRARRMALDPRFQPVILRRL"
     gene            complement(8954..9985)
                     /locus_tag="PK144_06450"
     CDS             complement(8954..9985)
                     /locus_tag="PK144_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNF65402.1"
                     /translation="MLLLPVLVATTAGAAEDGQPRAAGEVRQWLERTLGGVERLNFEG
                     TFVYVQGSHVESMRITHGRADTGEWQRLNSLSGPKREVLLANRDLTCSLPQGQITLKS
                     RRVTSFPVSLPKDIDRLERHYAFGLQGEDRVAGHEARILAVTPRDGLRYGLRLWLEKN
                     TGMIVRTMLVGSDGAPLEQMMYTDLRFLDVAPPAPSAAELQAAGAGDPGSGETPVSTV
                     AEVAPVAQWSLASPPAGFEQVQHNRFQRQSDGRPTEHVVYSDGLATISLFIERVTPGR
                     SLLEGPSRIGAMNAWGKVADGIQAVVVGEVPAATVAAFAQGLKFAPAPVTSTATTAGT
                     ADKTAETKP"
     gene            complement(10027..10566)
                     /locus_tag="PK144_06455"
     CDS             complement(10027..10566)
                     /locus_tag="PK144_06455"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNF65403.1"
                     /translation="MTDKTAEQLSALTDGELDSQELDLLVRRLERDEELAARWTRYHL
                     IGEAMRNNLPEQLPFDLTARVSAAIAEEAPVVAPAVATRGWKRPVVGLALAASIAAAA
                     LIGLPPQQALQPEAGAPVAVQTPEKLPGAAAGQVAQVDWERNARLNEFLMSHNESATM
                     NQMSGVIPYARMVGYEEER"
     gene            complement(10580..11158)
                     /gene="rpoE"
                     /locus_tag="PK144_06460"
     CDS             complement(10580..11158)
                     /gene="rpoE"
                     /locus_tag="PK144_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNF65404.1"
                     /translation="MGESVADQELVLRVQKGDKRAFDILVSKYQYKIVKLVTRYVHDQ
                     TEALDVAQEAFIKAYRALSGFRGDSAFYTWLYRIAINTAKNYLVAQGRRPPGSDIDAT
                     EAEQYESAALLKEIDTPERLLLRDEIEATIFRAIEALPEDLRTAITLREIEGMSYEEI
                     AQSMGCPIGTVRSRIFRAREAIDRHLRPLVDG"
     gene            11363..>11631
                     /locus_tag="PK144_06465"
     CDS             11363..>11631
                     /locus_tag="PK144_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF65405.1"
                     /translation="MTGHFAFDVLIIGSGAAGLSLALRLAEHAKVAVLAKGPLSEGST
                     NYAQGGVSAVFDAADSIESHIQDTLIAGADLCHEGAVRFTVEHGPA"
     assembly_gap    11632..11731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11732..>12823
                     /gene="nadB"
                     /locus_tag="PK144_06470"
     CDS             <11732..>12823
                     /gene="nadB"
                     /locus_tag="PK144_06470"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNF65406.1"
                     /translation="LTDNRCVGAYVLDRDSGRIEVIAARFVILASGGASRVYLYSSNP
                     DGSTGDGIAMAWRAGCRIANMEFMQFHPTCLYHPAAKSFLITEAVRGEGGHLLLPDGT
                     RFMERFDPRLELAPRDIVARAIDHEMKRLGVPCLYLDISHKPAAFIEEHFPNVLATCL
                     DYGFDMRREPLPIVPAAHYTCGGVMTDFAGHTDLPGLYAVGEVAFTGLHGANRMASNS
                     LLECLVFAASAGQDILTRLADVPPPPPLPDWDESRVTDSDEEVVVSHNWLELRRFMWD
                     YVGIVRTDKRLARARHRVELLLREIDEYYGHFRISADVIELRNLAQVAELIIRCALAR
                     QESRGLHYTLDYPETDTAHPPRDTVLVPGN"
BASE COUNT         1893 a         4417 c         4423 g         1990 t
ORIGIN      
        1 caaggcccgg ccccttgcac gcaaggcttt ttcccttgcg cttcgcgtgg aaagccttgc
       61 gcttcgcgtc agaagccttg cgaccgacac tcgaagcctc gcgaccggcg cccgcagcgt
      121 ccgcgacgcc gccggccgcc gcgagcctgg ttcaggccgg cgcgcggccg ggcgggccgg
      181 cgtcgccctc gacgatcacc accgccagcg cgtattcgcg ctcgtcgctg atgctgacgt
      241 gcgtggcgct ggcgccgagc gcctgcaggc gtgcggccgc agcaccgctg aggatcaggt
      301 gcgggcagcc gagcgcgtcg tggccgaggc cgacctgcgc gagcgtcagg ccctgctgca
      361 cgccggtgcc gagcgccttc agcgccgcct ccttgactgc gaagcgcttg gcgagcagcc
      421 gtgccgggtg gcggctggcg cggtactcgt cgagctcgtc ggcggtcagc accttctcgg
      481 cgaagcgttc gccgtggcgg gcgatgatct cctccatgcg cgcgacgcgc acgatgtcgg
      541 tgccgagccc gaggatcatc ggccggcgac cctcgcgcgg gcctccagca tccgccgctt
      601 catgtcgcgc acggcctgcg cgagaccgtc gaacagcgcc tgcgcgacga tggcgtggcc
      661 gatgttgagc tcgtgcagct cggggatcgc cgcgatggcg gcgacgttgt ggtagtgcag
      721 gccgtggccg gcgttgacga ccagcccgag cgcatgcgcc tgcgcagcgg cggcgcgcag
      781 gcgcgcgagt tcggtcgcga gctcgtcggc ggtggcggcc tcggcgtagc ggccggtgtg
      841 cagctcgatc ttcggcgcgc cgctgcgcgc ggcggcgtcg atctgctccg ggtcggcgtc
      901 gatgaacagc gacaccgcgc agccggcttc ggcgagccgc tcgcaggcct cgcgcacgcg
      961 cgggaagccg ccggcgacat ccagcccgcc ctcggtggtc agctcctgac ggcgctccgg
     1021 cacgaggcag acatcggccg ggcgcaggcg cgcggccagc gccagcatcg gtgcggtgac
     1081 ggccatctcg aagttcatgc gcgtctgcag cacctgcgcg atcagctcga catcgcgatc
     1141 ctggatgtgg cggcggtcct cgcgcaggtg cagcgtgatc gcgtcggcgc cggcctgttc
     1201 ggcgaccagc gccgcctgca ccggctccgg gtagcgcgta ctgccgcgcg cctggcgcag
     1261 cgtcgcgacg tgatcgatgt tgacgccgag cagcagcggg gtcggggcgt tcacgcgggt
     1321 tttcctccgg cgggggcggc gaggctgcgg aaagcgcgcg cgagttcgcg cgtgcgcacc
     1381 ggccggccgc cgagacagtg atcgatcagc gagcgcgtca gcgtgcgcgc ctcgcgcagc
     1441 gtgtcgcggt ccggacaggt gaatgattcg gcaccggcgg cgagcgcgag cagcgtgcgg
     1501 ccgctgagct cgacgccgcc ggccggcgct tcggcgaggc ccggcagccg ccgggcgccg
     1561 gcatcgggcc ggtagtgata gcgggcatcg gcctgcagcg gctcgccgtc ggtatcgacc
     1621 gccagcgccg gtgcgtagcc gagcgcggtg agcagctcgc actcgaacac gcgcagcgcc
     1681 ggttccgggt cggcaggttc ggcgagcgcc accagcgcgg cgcggtaatg gccgagcagc
     1741 agcggctgcg gatcgtcgcg ggcgagcagc ttcagcagca gttcgttcag gtacaggccg
     1801 gcgacggcgc gctcgccggc gagccagaag gcggcaccgg cggcctcggc gctcgtgagg
     1861 gtcgccagtt cgccgcgccc ggcaagcccg accagcagcg gctggaacgg ccgcagccag
     1921 ccgcggctgc gcgaacgcgg tccgcgcgcg ccacgcgcca ccgcgccgac ccggccgcgc
     1981 gtcagcgtca gcagttcgag cagcaggctg gtgtcgcggt acggtcgcgt gtgcagcacc
     2041 caggcgggtt cgagtccggg ggacatcacg ccagccgccc ggcttcagtc ctccgactcg
     2101 tcgaagccga ggctgcgcaa ggctttttcg tcgtcggacc agccttcgcg caccttgacc
     2161 caggtctcca ggtgcacctt ggcgtcgaac atctcttcca tgtcgaggcg cgccagccgc
     2221 ccggcttcct tgagcgtgcc gcccttgtcg ccgatgacga tggccttctg gcccttgcgc
     2281 tcgacccaga tcaccgccga gatccggtgc agccggccct cgacctcgaa cttctcgatc
     2341 tcgacggtca gcgcatacgg cagctcgtcg gagagcacgc gcgtgagctt ctcgcgcacc
     2401 agctcggcgg cgaggaagcg cttgctggcg gtggtgatct ggtcctcgtc gaagaagaac
     2461 ggattggccg gcaagtgctt cgcgatctcg gtttccagcg ccgcggcgtt cgtgcccttc
     2521 tgggccgaca gcggcacgat cgccgcgaac gcgcgctttt cggccatctg ctgcaggaag
     2581 ggcagcagct tcggcttgtc ggtgacgcga tcgaccttgt tgacggcgag cagcaccggg
     2641 ccggcgtagt cggcgaggcg ttcgagcacg gcggcatcct cgtcggtcca gcgcagcgcc
     2701 tcgatcacga acaccacggc atcgacgtag ccgagcacgt tgccggcggc gcggttcatg
     2761 tagcggttca tcgccttgcg cccgttcagg tgcaggcccg gcgtatcgac gtaaacgatc
     2821 tgcgtgtcgg cgagcgtctg gatgccgagg atggtgtggc gcgtggtctg cggcttcggc
     2881 gaggtgatgc tgagcttgaa ggcgagcagg cggttcagca gcgtcgactt gccgacgttg
     2941 ggccggccga gcagcgcgac gtagccggca cgggtgttcg gggcatcggc gtcgctgtcc
     3001 ggcgttgcgg tcggttcggt cggttcggtc atggtcacgg catccggacg ggcgccgccg
     3061 gcagggcggc gcgacaggga aaggaatgtt cagtggccgc cagcggtgag cgtcgccagc
     3121 atgcggccgg cggcttcctg ttcggccttg cgccggctcg tgccgctggc gacgacggcc
     3181 ggcaggccgc tgcaggtgca ttcgaccgtg aaggtctgcg cgtgcgcctc gccgctgact
     3241 tcgacgatgc ggtaggccgg cagcggctgc tggcgggcct gcaggtattc ctgcagccgc
     3301 gtcttcgggt ccttgagttc ggacggtgat ggcagcgcat cgagccgcgg accgaacagc
     3361 gccagcacga cgcggcggca ggtttcgaag tcgctgtcga ggtagatcgc gccgatcagc
     3421 gcctcgaagg catcggcgag gatcgaggcg cgccggtagc cgccgctctt cagttcgctg
     3481 ccgccgaggg cgagcgcatc gccgagcgcc agctcgcgcg ccagttcggc gagggtcgcc
     3541 tcgcgcacca gcagcgcgcg cagccgcgtc aggtcgcctt cgggcgcacg cggcaggcgc
     3601 tggtacagcg cctcgccgat cacgcagccg agcagcgcat cgccgaggaa ttcgaggcgc
     3661 tcgttgttgc tgccaccggc cgagcggtgc gtcagcgccg cgtcgcgcag cgccgggtcg
     3721 cggaagcggt ggccgagcgc tggctcaaga cgcgcagccg cgctcggcag gccggtatgc
     3781 atgccggcgc ccgcctcaga ggccggaggc agaggcgatc ttgacacgct gttcatgcgt
     3841 gaaatgcgca accacgtcga ggttgtagat cagcggccgg cgggtctcgt actcgatcga
     3901 gatcaccagt tcgccggcat ccttggtgac cttgacgtcg ctcgacttga tcgcgtcgat
     3961 gtcgttgacg tcgaagtgcc gctgcaggcg gttgcggatc tcgtacgccg acatccgcgc
     4021 cagattggct tcctgctcga tgcccttcat cgcgctgctg accgtcgcgt ccgcgatgta
     4081 gatcggtgcg atccggaaca ccatcagcat gcagaagccg atcacgccgc agaccagcag
     4141 cacggtgatc ggcgacatgc cgcgctggcc cgcccgcagc gcgcgggcgc agggacgggg
     4201 ctgctgcctt ctcattcgat cctcctgccg atgcggccgc agtggccgtt gccggtcacg
     4261 caatcccagt tcatccagat catgaacgcc ttgccgatca ggttctccgc cggcaggtaa
     4321 ccccagtagc ggctgtcgcg gctgttgtcg cggttgtcgc ccatcgcaaa atagtggccc
     4381 ggcggcacga cgtactcgcg cgccccgctg tcgagcatgc gcggcgccac ctggatcagg
     4441 tgcttcacgt cgccgagctg ttcctggcgc gcctcgaacc ccggttcgag gtcgctgacc
     4501 gggccgggca gcttcgtctg ctcgaccgcc tgcccgttca cgaacagctg cttgtcgcga
     4561 tagaccagcc tgtcgccggg caggccgacg atgcgcttga tgaaggccgt cgacgggtct
     4621 tccggatagc ggaacaccgc cacgtcgccg cgcttcacct ctccggtcga caggatctcg
     4681 gtgttgctga ccggcaggcg caggccccac gagaacttgc tgaccaggat gaagtcgccg
     4741 gcgcgcagcg tcggcagcat cgagccggac gggatgcgga agggctcgac gatgaaggag
     4801 cgcagcagca gcacgacgac gatgaccggg aagaaggatc gcgaccagtc gacgtaccac
     4861 ggcatcggcc gcggcccgcc gaccccgccg gcggcggccc gggccggtgc cagcttccag
     4921 acatcgaggg catagaccag accggtcacg agtgcggcga gcaccagcgc ggcagcaaaa
     4981 tcgaaagtca tcgaaccgga ttcctcttgc ttgcgggcgt ggagtctggc gccgggagcg
     5041 gcggctcatg ccgtccccgg cggaaggaag ccaccgacga tttcagcccc ctgcgctcac
     5101 cgcagggcgg tcgggaaacg ggggaaggcc aggcatgccc ggtgcggccg gcatcacttc
     5161 ttgtcgacct gcagcacggc gaggaaggcc tcctgcggga tgtcgacctt gccgaccgat
     5221 ttcatgcgct tcttgccttc cttctgcttt tccagaagct tgcgcttgcg cgacacgtcg
     5281 ccgccatagc acttggccag cacgttcttg cgcagggcct tgaccgtcga gcgcgcgatg
     5341 atgtggctgc cgatcgccgc ctggatcgcg acctcgaaca tctggcgcgg gatcagatcc
     5401 ttcatcttct cgaccagatc acggccgcgg tacggcgcct gctcgcgatg cacgatgatc
     5461 gccagcgcat cgaccttctc gccgttgatc agcacgtcga gcttcaccag cggctcggcc
     5521 tggaagcgct cgaagctgta gtcgaaggac gcgaagccgc gcgacaccga cttcaggcgg
     5581 tcgaagaaat ccagcacgac ctccgacatc ggcagctcga aggacagctg cacctgaccg
     5641 ccggcggcgt agtggaggtt gcgctgcacg ccgcgcttct cgatgcacag cgtgatgacg
     5701 ttgccgaggt agtcggccgg caccaggatg ttggcgagaa tgatcggctc gcggatctcg
     5761 tcgaggtagt tcgacgccgg cagcttgtcg ggattgtcga tgtaggcgat ctcgccggcg
     5821 gtggtcagca cctcgtagac cacggtcggc gcggtggtga tcaggtcgag gtcgtactcg
     5881 cgctccagcc gttcctggat gatctccatg tgcagcatgc cgaggaagcc gcagcggaag
     5941 ccgaagccga gcgcctgcga ggtctcgggc tcgtagtgca gcgaggcgtc gttcaggcgc
     6001 agcttcgcga gcgcctcgcg caggtcctcg tactggtcgg attcgatcgg gaacagcccg
     6061 gcgaacacgc gcggctgcac cttctggaag ccgggcagcg ccgccgcggc cggattctgc
     6121 gcatcggtga tcgtgtcgcc gaccggcgcg ccgtcgatct ccttgatgtt gccgatcacg
     6181 aagccgacct cgccggcggc gagcaccggc ttgtcgaagc gcttgggcgt gaacacgccg
     6241 accttgtcga cctggtagac gcgatcggac gacatcatcc ggatcttctg gcgcggtgcg
     6301 atgcgtccgt cgacgacgcg caccagcgac acgacgccga cgtaggcatc gaaccaggaa
     6361 tcgatgatca gcgccttcag cggcgcatcg ggatcgccct tcggggccgg gatgcgctcg
     6421 acgagctgtt cgagcaggtc gacgacgttc aggccggtct tggccgacac gcgcacggcg
     6481 tcggaagcgt cgatgccgat gatgtcgccg atctcgtcga tgacacgctc gggctcgcac
     6541 tgcggcaggt cgatcttgtt cagcaccggc agcacttcga ggccctgctc gatcgccgtg
     6601 tagcagttgg cgaccgactg cgcctcgacg ccctgcgcgg catcgaccac cagcagcgcg
     6661 ccctcgcagg cggcgagcga acggctgacc tcgtagctga agtcgacgtg accgggcgtg
     6721 tcgatgaagt tgagctggta ggtctcgccg ttgcgtgcgg tgtaatcgag cgacaccgac
     6781 tgcgccttga tcgtgatgcc gcgctcgcgt tcgaggtcca tcgagtcgag cacctgcgcg
     6841 gacatctcgc gctccgacag gccgccgcag atctggatga tgcggtcgga gagcgtggac
     6901 ttgccgtgat cgatgtgggc gatgatcgag aagttgcgga tgtgatccat cgaggccatg
     6961 ggtgcgaagg ttccggcggg cccgcgcagg gactgcgcag gcgaagggag agcgggccgg
     7021 cgcttgccgc gtcagggcac gcgcagggcc agataggcgg gtccggagcg gcgcagcacc
     7081 agcagcgcga tggcacggcc ggccggcagg tcttcgagcg tgcggtcgaa ggcatccgga
     7141 tcccgaatct gcgcgccgtt gagcatgccg agcacgtcgc cctggcgcag gccggcacgg
     7201 gcggcacggc tgttgtcgac ctgctcgacc agcacgccgc cctcggggat gccgagctgg
     7261 cggcgcagct ccggcggcac cggcgcgacg cgcaggccca gccggtcgag cggccgctcg
     7321 gaggtgacga ccggctcggt gctcgcgacc ggcggctcgt tgtcgggcag cgccgcgatc
     7381 tgcaccgcca gcgtctgcgg cttgccgccg cgcatgatcg cgagttcgac gcgccggtcg
     7441 acgccggtcg cgccgaccag cggcggcagc gccgtcgaca ggtcgatctc gtggctgtcg
     7501 aagcgcgtga tcacgtcacc ggtgcgcagg ccggccgcgg ccgccggacc gtccgggaac
     7561 acgcggctga ccagcgcgcc gcgcgggctc gcgagcccga aggattcggc cagatcgcgg
     7621 gtcagcggct gcaccagcac gccgagccag ccgcggctga cgtgaccgct gtcacgcagc
     7681 tggcgcgcca cgtccatcgc cgtctcgatc ggaatcgcga acgacaggcc catgtagccg
     7741 ccggtctggc tgtagatctg cgagttgatg ccgatgacct cgccgcgcag gttgatcagc
     7801 ggtccgccgg agttgccggg gttgatcggc acgtcggtct ggatgtaggc gacgtagggc
     7861 tcgctcggca ggctgcggcc cttggcactg acgatgccgg cggtcaccga ctgctcgaag
     7921 ccgaacggcg aaccgatcgc gagcacccac tcgccgactt ccagcgcatc ggcccgcccg
     7981 accgtggccg gctgcaggcc gtcggcgtcg atcttcagca gcgcgaggtc ggtgcgttcg
     8041 tcggtgccga tcacgcgcgc cggcagttcg cgccggtccg gcaggcgcac gacgatgtcc
     8101 tcggcatcgg cgatcacgtg acggttggtc agcagccagc cgtcggccgc gatcacgagg
     8161 ccggtcccga gcgcatcggc agcgtccgga gaagcgtctt cgtcaccgtc atcgctgttg
     8221 tcatcggccg ccggctcggt cgcggccgcg agcttgccga tcgagctgat gttgacgacg
     8281 gaaccggcat tgcgcttcac gagtgccgga aaatcgggca atccggccgc cagtgcaccg
     8341 gcacacccca gcagccacag cacgcccgcc agagcgcccc gccacacgac catgcacgac
     8401 tcccgccgtc acgacgaacc agaactggac ccgcgagccg cgggccggaa agcggcgagt
     8461 atacctgcgc actccggagg actgtgaata gccttcccgg cagctgcggc ggggatcagg
     8521 ggtcccggga ttcagagccg gcgcaggatg accggctgga agcgcgggtc cagggccatg
     8581 cgccgggcgc gccggcgcgt ccacagcagc gcaagcagca ggccggccat accgctcgcc
     8641 gcgctcgtgc cttgcccgaa cggcgagccg gcgagcgccc cgcccagcag gccgagcagc
     8701 ggcaccaggt acaggatcgc cgatccggcg agcagtgcgc cttcggccag accgagttcg
     8761 acgtgctcac cgctgcggta atcggcatcg gcgagcgcgg caacgacgtt gcgccgcccg
     8821 ccggtgacct tgccgatcgc agccgtgccg cagccggcct gggcctgaca gctgccgcag
     8881 gcgctgcgcc gccgggtttc gaccagcagc cggccgtcgg cagccggacc gagcacgatg
     8941 ccttcctcga cgatcaaggc ttggtttccg ccgtcttgtc ggccgtgccc gccgtggtgg
     9001 ccgtgctggt gacgggcgcc ggtgcgaact tcaggccctg cgcgaacgca gccaccgtcg
     9061 ccgccggcac ctcgccgacc acgaccgcct ggatgccgtc ggcgaccttg ccccaggcgt
     9121 tcatcgcgcc gatgcgcgac ggcccttcga gcaggctgcg gcccggcgtc acgcgctcga
     9181 tgaacagcga gatcgtcgcc agcccgtcgc tgtagacgac atgctcggtc gggcggccat
     9241 cgctctggcg ctggaagcgg ttgtgctgca cctgctcgaa gccggccggc gggctggcga
     9301 ggctccactg cgcgaccggc gccacctcgg cgaccgtcga caccggggtt tcgccgctgc
     9361 cgggatcgcc ggccccggcc gcctgcagct cggccgcact cggtgcgggc ggcgccacgt
     9421 ccaggaagcg caggtcggtg tacatcatct gttcgagcgg cgcgccgtcg gagccgacca
     9481 gcatcgtgcg cacgatcatg ccggtgttct tttcgagcca cagccgcagg ccgtaacgca
     9541 gaccgtcacg cggcgtgacg gcaaggatgc gggcctcgtg gccggcgacg cggtcctcgc
     9601 cctgcagccc gaaggcgtaa tggcgttcga ggcggtcgat gtccttcggc agactgaccg
     9661 ggaagctggt cacgcggcga ctcttcagcg tgatctgccc ttgcggcagc gagcaggtga
     9721 ggtcgcgatt ggccagcagc acctcgcgct tcggacccga cagcgagttc aggcgctgcc
     9781 attcgccggt gtcggcccga ccatgcgtga tgcgcatcga ttcaacgtgc gagccctgca
     9841 cgtacacgaa ggtgccttcg aagttcagcc gctcgacacc gccgagcgta cgctccagcc
     9901 actggcgcac ctcgccggcc gcccggggct gtccgtcttc cgcagccccg gcggtcgtcg
     9961 ccaccagcac cggcagcagc agcaccgccg cgcaggcggc tcgcttacat actgtgccgg
    10021 cgctgatcaa cgctcctcct cgtaaccgac catacgggca tacgggatga caccgctcat
    10081 ctggttcatc gtcgccgatt cgttgtggct catcaggaac tcgttcaaac gcgcgttgcg
    10141 ctcccagtcg acctgggcga cctgtccggc ggctgcaccc ggcagcttct ccggcgtctg
    10201 cacggcgacc ggcgcccccg cctcgggctg cagcgcctgt tgcggcggca ggccgatcag
    10261 tgcggcagcc gcgatcgagg ccgccagtgc cagtccgacg accggccgct tccagccccg
    10321 ggtggcgacg gccggcgcaa cgaccggcgc ttcctcggcg atcgccgccg atacccgggc
    10381 ggtgaggtcg aacggcagtt gttcgggcag gttgttgcgc atcgcctcgc cgatcaggtg
    10441 ataacgcgtc cagcgcgcgg cgagttcctc gtcgcgctcc agccggcgga ccagcagatc
    10501 gagctcctgg gagtcgagct caccgtccgt cagcgccgaa agctgttcag cagtcttgtc
    10561 cgtcatgtct tcagccctgc taaccgtcca ccagtggacg aaggtgtctg tcgatggcct
    10621 cgcgcgcgcg gaagatgcgg gagcggaccg taccgatcgg gcagcccatg gactgcgcga
    10681 tctcctcgta gctcattcct tcgatctcgc gcagcgtgat ggccgtgcgc agatcctcgg
    10741 gcaaagcctc gatcgcccgg aagatggttg cctcgatctc gtccctgagc agcagcctct
    10801 ccggcgtgtc gatctccttg agcagcgcag cactctcgta ctgctcggcc tcggtggcat
    10861 cgatgtccga tccgggcggt ctgcgcccct gggccacgag gtagttcttg gcagtgttga
    10921 tcgcaatccg gtacagccag gtatagaacg cactgtcacc acgaaacccg gacagtgcgc
    10981 ggtacgcctt gatgaaagct tcctgcgcaa cgtccagcgc ctccgtctgg tcgtgaacgt
    11041 agcgggtgac gagcttgacg atcttgtact ggtacttgct gaccaggatg tcgaacgcac
    11101 gcttgtcccc tttctgtacc cgaaggacca gttcctgatc tgccactgac tcgcccatgc
    11161 gggcaaaccc ctttgaccgt aaggcatcgt ccgtgcgagt cacctggtag accgcgtcaa
    11221 cggccccggg ttcgcaccga taccgggaag ttgattaaga tgatttgaag cagcggacgc
    11281 cgggatatag cagaagacat gccatgtcgc ccggctggcg acgcggctca aagtacccca
    11341 cgcccgaggc cccctcaaga tcatgaccgg tcactttgct ttcgacgtgc tgatcatcgg
    11401 cagcggtgcc gccggcctca gcctcgccct gagactcgcc gaacacgcaa aggttgcggt
    11461 actcgccaag ggaccgctgt ccgaaggcag caccaactac gcccagggcg gcgtgtcggc
    11521 ggtgttcgac gccgccgact cgatcgaatc gcacatccag gacacgctga tcgccggtgc
    11581 cgacctctgc cacgagggtg cggtgcgctt cacggtcgag catggcccgg cnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgaccgac aaccgctgcg tcggcgccta
    11761 cgtgctcgat cgcgacagcg gccgcatcga ggtgatcgcc gcgcgcttcg tgatcctcgc
    11821 gagcggcggt gcgagccgcg tgtatctgta ttcgagcaat ccggacggct cgaccggcga
    11881 cggcatcgcg atggcctggc gcgccggctg ccggatcgcg aacatggaat tcatgcagtt
    11941 ccacccgacc tgcctgtacc acccggcggc caagtccttc ctgatcaccg aggcggtgcg
    12001 cggcgaaggc ggtcacctgc tgctgccgga tggcacgcgc ttcatggagc gcttcgatcc
    12061 gcgcctcgaa ctcgctccgc gcgacatcgt cgcgcgcgcg atcgaccacg agatgaagcg
    12121 cctcggcgtg ccctgcctgt acctcgacat cagccacaag ccggcggcct tcatcgagga
    12181 gcacttcccg aacgtgctcg cgacctgcct cgactacggc ttcgacatgc gccgcgaacc
    12241 gctgccgatc gtgccggccg cgcactacac ctgcggcggc gtcatgaccg acttcgccgg
    12301 ccataccgac ctgcccggcc tgtatgcggt cggcgaggtc gccttcaccg gcctgcacgg
    12361 ggccaaccgc atggcgtcga actcgctgct cgaatgcctg gtgttcgcgg cctcggccgg
    12421 tcaggacatc ctgacccggc tcgccgacgt gccgccgccg ccgccgctgc cggactggga
    12481 cgagagccgc gtgaccgatt ccgacgagga ggtcgtcgtc tcgcacaact ggctggagct
    12541 gcggcgcttc atgtgggatt acgtcggcat cgtgcgcacc gacaagcgcc tggcgcgcgc
    12601 ccggcatcgc gtcgagctgc tgctgcgcga gatcgacgag tactacgggc acttccgcat
    12661 cagcgccgac gtgatcgagc tgcgcaacct cgcgcaggtc gccgagctga tcatccgctg
    12721 cgcgctcgcc cgccaggaaa gccgcggcct gcactacacg ctcgattacc cggaaaccga
    12781 caccgcgcac ccgccgcgcg acaccgtgct cgtgcccggc aac
//
LOCUS       DAOQIE010000074        12780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2394_length_12780_cov_7.233006, whole genome shotgun sequence.
ACCESSION   DAOQIE010000074 DAOQIE010000000
VERSION     DAOQIE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_12780_cov_7.233006"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="PK144_06475"
     CDS             complement(<1..1328)
                     /locus_tag="PK144_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955377.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNF65407.1"
                     /translation="MNAVAGIQGRERYRPVHKLRFVTAAALFDGHDASINLMRRILQA
                     QGAEVIHLGHNRSVESVVSAALDEDVQGIAVSSYQGGHVEYFRYMIDLLRARGGGHIR
                     VYGGGGGVIVPAEIAELQDYGVTRLFSPEDGQRLGLAGMINLMVEACDVDLAAQAPAT
                     LDGLRTGDRLALARWLSVLEAGRMAPALRDELLQAAQARRAPVLGITGTGGAGKSSLT
                     DELIRRFRLDRDEALKIAVLAADPVRRRSGGALLGDRIRMNAIEHPNLYLRSLATPAG
                     GSELPQRLDELVAACRVAGFDLVIIETPGIGQGAAGIVAHADLALYVMTPEYGAASQL
                     EKIDMLDFADVVAINKYDRPGGADALRDVRRQVQRNREDWGTPPADLPVFGTIASRFN
                     DDGVTALYQHLSERLAALGLPPAGGRLAAVTTRASTGSRALLPPARVRHLA"
     gene            complement(1549..2799)
                     /locus_tag="PK144_06480"
     CDS             complement(1549..2799)
                     /locus_tag="PK144_06480"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNF65408.1"
                     /translation="MNTDCLPDGDDRKQDRKNDAEILLLDDEPLLLRVLALALNACGY
                     RSITSCANAADALLRLDDAALPPIELIFCDLNMPGMDGVQFVRALGERGWQGSLVLIS
                     GEERRTVDTVARLASAHRLDLLGQMPKPVVRAQLVELLERWRTRQPAPGPGAQAGEVE
                     PGELRRALAAGEFVNVYQPKMAFAGGGIEGVEALVRWQHPVAGIIYPDRFIGVAERHG
                     LIDALTQAVCARALADASDWQRQGLGLSVAINVSMDSLRDLDFPERIAAEVRRAAFNP
                     AQLVLELTESRLPPNPVVLLDTLTRLRLKRYRLSIDDFGTGYSTLAQLRDIPFDELKI
                     DRGFVHRAHADPARGAILAASLGMAHQLGLRTVAEGIEDAADWQHVQRLGCDLGQGYH
                     IARPMPPAALPAWVAARSLSGQAR"
     gene            complement(2796..3632)
                     /locus_tag="PK144_06485"
     CDS             complement(2796..3632)
                     /locus_tag="PK144_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65409.1"
                     /translation="MLPLPAAAPPGIEAARTAGRLILVAEDNAINRKVLERQLALLGH
                     AAEFVADGRAALSRWRSHRHALLITDLHMPELDGYELAATIRHEESASGSRRRPIIAF
                     TANALKGEVEHCRSVGMDDCLIKPLQIERLRTALERWLPVASVPAAAAPVPEAPPPRS
                     GERPPPFDPLALQELIGDDAELITGLLDEFLDGVRANLDALRQLQADQDLTGIADIGH
                     RLKSSARAVGAAELGMLYEQLEQAGRADDGPAIDAVLAALAPAIARLTARIEPHAWNP
                     CT"
     gene            complement(4089..5450)
                     /locus_tag="PK144_06490"
                     /pseudo
     CDS             complement(4089..5450)
                     /locus_tag="PK144_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
     gene            complement(5469..7562)
                     /locus_tag="PK144_06495"
     CDS             complement(5469..7562)
                     /locus_tag="PK144_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF65410.1"
                     /translation="MTFSPRRLFWPLVLLASAVGLCGLLGIQYTNVRQLDSDAHAFAR
                     QIELLRNAFGYGGFIHHFKNFLLRRTQASYALAGAGLDSLGVTAAELEQFTHDLAERE
                     ALHEIGRVIGRYRTAWQLAGLPTHREMQPEALDGLVRVEDTVAISALGILEAAAQQRL
                     AQARAASTQLQMLLAASLVALPLLALVYLHQQRALRRESRLADCLAHSERELRQSNEQ
                     MRAIFDGMVHLICLLRPDGTLIELNRSAALFAGQARDTLLGLQLASLPWWPRSPDAQA
                     AVQRALAVAGSGQCFREEFPVLGPGGFRATLDVSLQPLRDEHGEIVRLVLEARDLSER
                     QRLEAGIRAEGERARRYLDIARSIILARSIDGRIQLINREGCELIGLPAGQLIGRNWF
                     ETCVPQGERTRAQVVFAQVITGFDPLIEEERALLTASGETRCIAWRHTVVCNETGVVV
                     AILSAGRDVSTERAAELHLRSINVELERRVRERTRALEDARDALLGRQHRLGILKTLA
                     EQAGSAADLSVVLNLTLRLVCEHLAWPVGHAWIRDAVDQPLRSSHCWHLGDPEIYAGF
                     QARSQALEFGPGVGVIGRVLEAGEAQWIEDLATDVHFLRHDPLVRSGLCGGGFCPVRV
                     RGEVRLLLEFFSTDDNGVTGEIRDFVHQVATLLGMIAETLLDRAELRRLALFASETDD
                     MLVVTDREGRIEWVN"
     gene            complement(7752..9698)
                     /locus_tag="PK144_06500"
     CDS             complement(7752..9698)
                     /locus_tag="PK144_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65411.1"
                     /translation="MTATLPQVPSTALESVPGSASLSSRWRNLPLRHQFHALLTIVVL
                     ALLLLVAGTLLGLRRDLDSQAREALAASLQSVMTVLEHFADAEGSARMDRAQAQARAI
                     EALTALRQGDTYVFAFDRDGRFVLHGGDPAQVGQPVRGLRDAEGRDLYEAFAGAAHRD
                     ASGSGRGDFVEYPYVRPSGTKPERKLSHVRTFEPWGWVLGTGVYLTDLDSRFARQRNT
                     LVAGVAIVAVLLGSLLTWQSRRIVGRVQAVLGFAQRLAVHDLQAHMRVDTQEEFGRMA
                     AALNAAVDSLREAAARDQAQMEQLRAAEAAREVQAREIAAQADCIREAAERDRAAADE
                     LRTKVGRIVVAVEAAASGELTVTPGVGGNDAIGRVGEAFGHLLHDLRSSIAGIGRNAE
                     ALGRSVEVARNTGQRLNGVAGDTSARAERLSGNASAVDDSLQSVASATEEMNAAVQEI
                     SRHVHRAVGIAAVAAQAADHATGLVGALTTSSAEIGSVSRTITGIAEQTKLLALNATI
                     EAARAGEAGKGFAVVANEVKELARGTEQATADIEKRIGTIQHDTGHVVHAITDIRRTI
                     DEVNEISAMIAAAIEEQSATTREISRSVALAAQGSAEITAFVRQLADTAQAVHTEAGA
                     TTQAADQIARIAEDQIQLIRRFRI"
     assembly_gap    9897..9996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10031..10921)
                     /locus_tag="PK144_06505"
     CDS             complement(10031..10921)
                     /locus_tag="PK144_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF65412.1"
                     /translation="MNHPMPCSGASAPTPDADQADVIAHWSRGTPRQWIQQRLAADGI
                     ERPGWRDVARYDQIHAGGLKAVATLAGLAGLGRGAGMVDLGGGLGGSARWLAGELGCR
                     VVCVDLTRTLVEAGAELTAWVGLDAQVQHLHADASATGLPAGGFDAVWVQHLALHLPE
                     PQALWQEAARLLRPGGVLALHEWTLGSGGEPYHPLPWSRADGTLSHLQTQAALAAGLR
                     AAGFAEAGFVDGSQAAADAYAAQRATLAKLECPDNPVLPADELLTVLANAERSLREGR
                     CHCVYATARRLGNDAITTAG"
     gene            11103..11840
                     /locus_tag="PK144_06510"
     CDS             11103..11840
                     /locus_tag="PK144_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65413.1"
                     /translation="MNDARLADLREQAVRLGYGLQTMLPVSAISAPGLASLAAPFRTL
                     WLVCNTGDAVQRRWRAGRDIEALLADNAATGAGSCGPLDDFCRAELGALLGTRAHRVL
                     HPHPASTAPLPLLALLAAAGWLRPGPFMNTLHARYGSWWAVRAVIALDAGADAPGEAL
                     GPSPCLDCSAPCVPACPAAAVGKPGWEAQRCLAQRVTIGSGCARRCIARESCPAGREW
                     RYEPATIAYHYGASQQTLQRWRGGERA"
     gene            11863..12150
                     /locus_tag="PK144_06515"
     CDS             11863..12150
                     /locus_tag="PK144_06515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNF65414.1"
                     /translation="MLTVIARIEARPEFAACLKQAMLALLAPTRAEPGCLAYELLESS
                     DDPALIFFHERWADRDALEAHWQSAHLLAYRDATKGWIDRRELTVLRPVEP"
     gene            12188..12466
                     /locus_tag="PK144_06520"
     CDS             12188..12466
                     /locus_tag="PK144_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002737686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNF65415.1"
                     /translation="MDFEWDETKALANLRKHGVSFAEASTLFGDPLELTIADPDHSHG
                     EFRFLSLGRSHAGRLLVVAYTERDHRIRIISARPATSREIQCHEAGRF"
     gene            12447..12683
                     /locus_tag="PK144_06525"
     CDS             12447..12683
                     /locus_tag="PK144_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65416.1"
                     /translation="MKPADSDSDTDTELRDEYDFSAAIRGRHHSDYQRGTNLVLLDAD
                     VAAAFPDAAAVNNALRLLLRLARQQLPPVPKPSE"
BASE COUNT         1918 a         4467 c         4350 g         1945 t
ORIGIN      
        1 gccagatgac gcacgcgcgc cggcggcagc agcgcgcggc tgccggtcga ggcgcgggtc
       61 gtgaccgctg ccagccgccc gccggccggc ggcaggccga gcgcggcgag ccgctccgac
      121 aaatgctggt acagcgccgt gacgccatcg tcgttgaagc gtgaggcaat cgtgccgaac
      181 accggcaaat ccgcaggcgg cgtgccccag tcctcgcggt tgcgctgcac ctgccggcgc
      241 acgtcgcgca gcgcatcggc cccgcccggg cggtcgtact tgttgatcgc gaccacgtcg
      301 gcgaaatcga gcatgtcgat cttttcgagc tgcgaggcgg cgccgtattc cggcgtcatc
      361 acgtacagcg ccagatcggc gtgcgcaacg atgccggccg cgccctgacc gatgccgggc
      421 gtctcgatga tgacgagatc gaagccggcg acccggcatg cggccacgag ctcatcgagc
      481 cgctgcggca gctcgctgcc gccggcgggc gtggcgagcg agcgcaggta gagattcgga
      541 tgctcgatcg cgttcatgcg gatgcgatcg ccgagcagcg cgccgcccga gcgccggcgc
      601 accggatcgg cggcgagcac ggcgatcttc agcgcctcgt cgcgatcgag ccggaagcga
      661 cggatcagtt cgtcggtcag cgaactcttg ccggcgccgc cggtgccggt gatgccgagc
      721 accggcgcac ggcgggcctg cgcggcctgc agcagttcgt cgcgaagcgc tggtgccatg
      781 cgtccggctt cgagcacgct gagccagcgc gcaagcgcga gccggtcgcc ggtgcgcagt
      841 ccatcgagcg tcgccggggc ctgcgcggcg agatcgacat cgcaggcctc gaccatcagg
      901 ttgatcatgc cggcgagccc gagccgctgg ccgtcctcgg gcgagaacag ccgcgtcacg
      961 ccgtaatcct gcagctcggc gatctcggcc ggcacgatga cgccgccgcc gccgccgtag
     1021 acgcggatgt gcccgccgcc gcgtgcgcgc agcaggtcga tcatgtacct gaagtactcg
     1081 acgtggccgc cctggtagga actgacggcg atgccctgca cgtcctcatc gagcgcagca
     1141 ctgacgacgc tctcgaccga acggttgtgg ccgaggtgga tcacctcggc gccctgggcc
     1201 tgcaggatgc ggcgcatcag gttgatcgag gcatcgtggc cgtcgaacag cgccgcggcg
     1261 gtcacgaagc ggagcttgtg gacggggcga taccgctcgc gcccctgaat cccggcgact
     1321 gcgttcatcc ggcacgtctc ctctgccccc cggtggatcg ggatgcgcct ctggcgggcg
     1381 ctgcggcgct gcgaccgggc ggaccggcgc acgccaatgc atcggatcat aagaacgccg
     1441 gaggccggtc acgtaaacat ctatttacga gcagttcccg ccccccgtcc cgccttgcct
     1501 tgcgcgaccc gatggcccct gcccgtcgcc cttgctgcct tgcctgcgtc agcgggcctg
     1561 accggacagg ctccgggccg cgacccatgc cggcagcgcc gccggcggca tcggcctggc
     1621 gatgtgatag ccctgcccca gatcacagcc gaggcgctgc acgtgctgcc agtcggcggc
     1681 atcctcgatg ccctcggcca ccgtgcgcag cccgagctgg tgcgccatgc cgaggctggc
     1741 tgcgaggatc gcaccgcgcg ccggatcggc gtgcgcgcgg tgcacgaagc cgcggtcgat
     1801 tttcagctcg tcgaagggga tgtcgcgcag ctgcgcgagc gtcgagtagc cggtgccgaa
     1861 gtcatcgatc gacagcctgt agcgcttcag gcgcagccgg gtcagcgtat cgagcagcac
     1921 gacggggttc ggcggcagcc ggctctcggt gagttccagc acgagctgcg ccgggttgaa
     1981 ggcggcacgg cgcacctcgg cggcgatgcg ctcgggaaag tccagatcgc gcaggctgtc
     2041 catcgagacg ttgatcgcca ccgacagccc gaggccctga cgctgccagt cgctcgcatc
     2101 ggccagggcc cgggcgcaca ccgcctgcgt cagcgcgtcg atcaggccgt gacgctcggc
     2161 aacgccgatg aagcgatccg ggtagatgat gccggcgacc ggatgctgcc agcgcaccag
     2221 tgcctcgacg ccttcgatgc cgccgccggc aaaggccatc ttcggctggt agacgttgac
     2281 gaactccccg gccgccagcg cccggcgcaa ttccccgggc tcgacctcgc cggcctgggc
     2341 accgggtccg ggcgccggct gccgcgtgcg ccagcgttcg agcagttcga cgagctgcgc
     2401 ccgcacgacg ggcttcggca tctgccccag caggtcgagc cggtgggcgc tggccaggcg
     2461 ggcgaccgtg tcgacggtgc gccgctcctc gccgctgatc agcacgaggc tgccctgcca
     2521 cccgcgctcc ccgagcgcgc gcacgaactg cacgccgtcc atgcccggca tgttgagatc
     2581 acagaagatc agttcgatcg gcggcagtgc ggcatcgtcg agccgcagca gcgcatcggc
     2641 ggcgttcgca cagctcgtga tgctgcgata gccgcaggcg ttcagcgcca gcgccaggac
     2701 gcgcagcagc agcggctcgt cgtcgagcag caggatttcc gcgtcattct tccgatcctg
     2761 tttccggtca tcgccatccg gcaggcaatc cgtgttcatg tgcagggatt ccaggcatgg
     2821 ggttcgatcc gggccgtcag acgcgcgatg gccggggcaa gtgccgcaag cacggcatcg
     2881 atggccggcc cgtcatcggc ccgcccggcc tgttcgagct gctcatagag catcccgagt
     2941 tcggctgcgc cgacggcacg ggccgaggat ttcagtctgt gtccgatatc ggcgatgccc
     3001 gtcaggtctt gatctgcctg cagttgccgc agcgcatcga gattcgcacg gacgccgtcg
     3061 aggaattcat cgagcagccc ggtgatgagt tccgcgtcat cgccgatcag ttcctgcagc
     3121 gcgagcggat cgaacggggg cggccgttcg ccggaacggg gcggcggcgc ttcgggaacc
     3181 ggggccgcgg cggcggggac gctcgcgacc ggcagccagc gttcgagcgc cgtgcgcagc
     3241 cgctcgatct gcagcggctt gatcaggcag tcgtccatgc cgacgctgcg acagtgttcg
     3301 acttccccct tcagcgcatt ggccgtgaac gcgatgatcg gccggcgccg gctgccgctg
     3361 gcgctttcct cgtgccggat cgtggcggcg agttcgtagc cgtcgagttc cggcatgtgc
     3421 aggtcggtga tcagcagcgc atgccggtgg ctgcgccagc gggagagcgc agcgcggccg
     3481 tcggcgacaa actccgcggc atggccgagc agcgccagct ggcgttcgag gaccttgcgg
     3541 ttgatcgcat tgtcctcggc gaccaggatc agccggcctg cggtgcgggc agcttcgatg
     3601 cccggcggcg cagctgccgg cagcggcagc atggtttcga ccgctggtgt gaccggcgag
     3661 gccaggccgg ccgcgatggc caccgcgcgc aggaatacat ggcggtgcag cacgttgcca
     3721 tcgagcgtga tcaggtcatc gccttcgatg cggctttcgc gccgcctgcc gtgcgtgatc
     3781 agcacgtagc gcacgtcacg ccggcccgcc tgcgccgcga actgcccgcg caggcgttcg
     3841 atgctgtcgg cgccgtggca gcgaccgtcg acgaccacga tcgcgagccg ggcatcgcct
     3901 gccgccgcac tgtcctgcac gagcgtatcg acgtcggccg ccggcagtac ccgcgcaccg
     3961 gcggcggcga gatattcgcc gagatcggcc ccgagccggt cgccgccgca gagcaggctg
     4021 accggcaggc cggcgaaggc gtagcgcggg gcaatcgacg gctgcccgcc ggccgcctca
     4081 agcggcagct cgatcgtgaa cgtcgagcca agcccgggcg tgctgtcgac gccgatcctg
     4141 cctcccatca gatcgatgag tcgggccacg atcgacaggc cgagaccggt gccgccgaag
     4201 cggcgcgtcg tcgaggcctc ggcctgcatg aacggctgga acaggcgctc gatggtctgt
     4261 gcggacatgc cgatgccggt gtcgatgacg cgcaggcgca gaccgccggc cgttccgcac
     4321 tcggcgcgca aggccacgca gccggggcga tccgtttgct ctccggtgaa cttcacggcg
     4381 ttgccggcga ggttgtagag cacctgccgc aggcgcacgg catccccgag gatccactcg
     4441 ggcaggcgcg gatcgacgta gatttccagc gtgacctgct tgctggccgc gatcggcgcg
     4501 agcgtgtcgc cgatgctctc gatgatggcc gcgagcggca gcggcgcgtg ctcgagcgca
     4561 aggtggccgg cctcgatctt cgagaagtcg agcacgtcgt cgatgatcga cagcagcgtg
     4621 tgcgcggact ggcggatcgt ggccacggtg tcggtctggg cgggcgtgag cgtttcgtga
     4681 ccgagcacct ccagcatgcc gatgacgccg ttcatcggcg tgcggatctc gtggctcatc
     4741 gtcgcgagga aggtcgactt ggcgcggttg gcctgctcgg cggcatcgcg ggcccgctcc
     4801 agttcggcgg tgatgcgccg tcgctcggcg atgtcgcgca cgatgccgat gaagcagcgc
     4861 tcgccgttca gctcgcactc gttgaccgcc agatcgatct cgagcctgcg gccgtcgcga
     4921 tgcaggccct cgacctcgcg gccgatgccg atgatcgccg cccgcccgcc ggcgacatag
     4981 cgcgcgagat agccgtcatg cgcactgcga tgcggctcgg gcatcagcat ggacacgttg
     5041 tgcccgatca cctcggcggc ttcataaccg aacagccgct cggccgccgg attgaaggcg
     5101 cggacgcgcc ccttcgcatc catcgtgatg atgccgtcga tgacgttctc gaacagcgag
     5161 cgcagttcct gctcgcggct gcgcagcagt tcatcggcct ggtgacgctg gctgatgtca
     5221 tgggcgaccg acaggtagcg ttcgagccgg ccggcggcgt cgtataccgg ctgcacggtc
     5281 agcgccaccc agaagggctc gccgctgcgc cggtagacga cgagttcggt gccgatcttg
     5341 tcccctgccc gcatcgcggc ctgcatgccg gcgatggttc cggcgtcggt gcggggaccg
     5401 tagatcaggt gtccggggtg ctgtccgagc gcctcggcgc acgtccagcc ggtgcagtcg
     5461 ctgaagccct agttgaccca ctcgatgcgt ccctcgcgat cggtgacgac gagcatgtcg
     5521 tcggtttcgc tggcaaagag cgcgagccgg cgcagttcgg cgcgatcgag cagggtctcg
     5581 gcgatcatgc cgagcagcgt cgcgacctgg tggacgaaat cccggatttc cccggtcacc
     5641 ccgttatcgt cggtcgagaa gaactccagc agcagccgga cctcgccgcg gacccgcacc
     5701 ggacagaaac cgccgccgca gaggccggaa cgcaccaggg ggtcatgacg caggaaatgc
     5761 acgtcagttg ccagatcctc gatccactgc gcctccccgg cctcgagcac acggccgatg
     5821 acgccgacgc ccggcccgaa ttccagcgcc tgcgaacgcg cctggaagcc ggcatagatt
     5881 tccggatccc cgagatgcca gcagtggctg gagcgcagcg gctgatcgac ggcatcgcga
     5941 atccaggcat ggccgaccgg ccacgcaaga tgttcgcaga ccaggcgcag cgtcaggttc
     6001 agcaccacgg acagatcggc cgcgctaccc gcctgttcgg ccagcgtctt caggatgccg
     6061 aggcggtgct ggcgcccgag cagcgcatca cgggcatctt ccagcgcccg ggtccgctcg
     6121 cggacacgcc gctccagctc gacattgatc gagcgcagat gcagctccgc ggcccgttcg
     6181 gtgctcacgt cgcgtccggc gctcaggatg gcgaccacga cgcccgtctc gttgcagacc
     6241 accgtgtgcc gccaggcaat gcagcgggtc tcgccgctcg cggtcagcag cgcgcgttcc
     6301 tcctcgatga gcggatcaaa gccggtgatc acctgggcga acacgacctg cgctctcgtg
     6361 cgctccccct gcggcacaca ggtttcgaac cagttccggc cgatgagctg cccggccggc
     6421 agaccgatca gttcgcagcc ttcgcggttg atgagctgga tgcgcccgtc gatgctgcgc
     6481 gccaggatga tcgagcgggc gatgtcgaga tagcgccgtg cccgctcgcc ctcggcgcgg
     6541 atgccggcct ccagacgctg gcgttcgctc aggtcacgtg cctccagcac cagccgcacg
     6601 atttcgccgt gttcgtcgcg cagcggctgc agtgaaacat cgagcgtcgc ccggaagccg
     6661 cccggtccga gcaccgggaa ctcctcgcgg aagcactgac cgctgccggc cacggcaagc
     6721 gcgcgctgca cggccgcctg cgcatcgggc gagcgcggcc accacggcag gctggccagt
     6781 tgcaggccga gcagcgtgtc gcgcgcctgg ccggcgaaca gcgccgcgct gcggttgagc
     6841 tcgatcagcg tgccgtccgg gcgcagcaga cagatgagat ggaccatgcc gtcgaagatg
     6901 gcccgcatct gctcgttgct ctggcgaagc tcccgttccg agtgcgccag acagtcggcc
     6961 agacgactct cgcgtctcaa cgcccgctgc tggtgcagat acaccagtgc cagcaagggc
     7021 agcgcgacca gactggctgc cagcagcatc tgcagctgcg tgctggccgc gcgtgcctgc
     7081 gcgagacgct gctgggcagc ggcctcgagg atgccgagcg ccgatatcgc cacggtgtct
     7141 tccacccgca ccagcccgtc gagggcctcg ggctgcatct cgcggtgcgt gggcaggccg
     7201 gcaagctgcc aggccgtgcg atagcgcccg atcacccgcc cgatttcatg cagggcctcg
     7261 cgctcggcga gatcatgcgt gaactgctcg agttcggccg ccgtgacgcc gagcgaatcg
     7321 agtccggccc cggcgagcgc atagctcgcc tgcgtgcgcc gcagcaggaa gttcttgaaa
     7381 tgatgaatga agccgccgta gccgaaggca ttgcgcagca gctcgatctg gcgggcgaat
     7441 gcgtgtgcgt cgctgtcgag ctgccgcaca ttcgtgtact ggatgccgag caggccgcag
     7501 aggccgactg cactcgccag cagcacgagg ggccagaaca ggcggcgtgg agaaaaggtc
     7561 atggatcggc atcctgctac acatgttgtc gcaccggccc gatcgtaccg acgccccgct
     7621 cccggcattt ccgccaggcc ggcggatcgc atcttttgac atattgccac ccctgccatc
     7681 gaggccggca cggctcgggc aggccgggaa ccgtccgcga ccggtccccg gcgggcagga
     7741 cgcgcttgcg ttcagatgcg gaagcgcctg atcagctgga tctgatcctc ggcgatgcgg
     7801 gcaatctggt cggccgcctg cgtcgtcgcc cccgcttccg tgtgcaccgc ctgagcggta
     7861 tcggccagct ggcgcacgaa agccgtgatc tcggcgctgc cctgcgcagc cagagccacg
     7921 ctgcggctga tctcgcgcgt ggtggcgctc tgttcctcga tcgcggcggc gatcatcgcg
     7981 gagatttcgt tgacctcgtc gatggtgcgg cggatgtcgg tgatcgcatg cacgacatgg
     8041 ccggtatcgt gctggatcgt gccgatgcgc ttctcgatgt ccgcggtcgc ctgctcggtg
     8101 ccccgcgcaa gctccttgac ctcgttggcg acgacggcga agcccttgcc ggcttcaccg
     8161 gcccgcgcgg cctcgatcgt ggcattcagg gcgagcagct tcgtctgttc ggcgatgccg
     8221 gtgatcgtgc ggctgaccga cccgatctcg gcgctgctgg tcgtgagggc cccgacgaga
     8281 ccggtggcgt gatcggcggc ctgcgcagcc acggcggcaa tcccgaccgc ccggtgcacg
     8341 tggcgcgaaa tctcctgcac ggctgcattc atttcctcgg tcgcgctggc gaccgactgc
     8401 aggctgtcat ccacggcact ggcgttcccg gacagccgtt cggcacgcgc actggtgtcg
     8461 ccagcgacgc cgttcagacg ctgaccggta ttccgggcca cttccaccga acgcccgagt
     8521 gcctcggcgt tgcggccgat gccggcaatg ctgctgcgca ggtcgtgcag cagatgcccg
     8581 aaggcttcac cgacccggcc gatggcgtca ttgcccccga cccccggcgt gacggtcagt
     8641 tcgccgctgg cagccgcctc gaccgcgacg acgatgcgtc cgaccttcgt gcgcagctca
     8701 tcggctgcgg cccggtcgcg ctcggccgcc tcccggatgc agtcggcctg agcggcgatt
     8761 tcacgggcct ggacttcccg ggcggcttcg gctgcgcgca gctgctccat ctgcgcctga
     8821 tcgcgggcag cggcttcccg caaggaatcg acggcggcat tgagtgcggc tgccatccgc
     8881 ccgaattcct cctgcgtatc gacgcgcatg tgcgcctgca gatcgtggac ggcgagccgc
     8941 tgcgcaaagc cgagcacggc ctgcacgcgt ccgacgatcc gccggctctg ccaggtcagc
     9001 agactgccga gcagcacggc aacgatggcg acgccggcca ccagcgtgtt gcgctggcgc
     9061 gcgaaccggg agtccagatc ggtcaggtag accccggtgc ccagcaccca gccccagggc
     9121 tcgaaggtcc ggacgtgcga gagcttgcgc tccggcttcg tgccgctcgg gcggacatac
     9181 ggatattcca cgaaatcgcc ccgcccgctg ccggatgcat cccggtgcgc agcgcctgca
     9241 aaagcctcgt acaggtcacg gccctcggca tcgcgcagcc cccggaccgg ttgcccgacc
     9301 tgcgcgggat cgcctccatg cagcacgaaa cgtccatcgc gatcaaaggc aaacacatac
     9361 gtatcgccct gacgcaaggc ggtcagcgcc tcgatcgcac gtgcctgtgc ctgcgcacgg
     9421 tccatccgtg cgctgccttc ggcgtcggcg aaatgctcaa gcacggtcat cacggactga
     9481 agcgaggcag ccagggcttc acgagcctgt gaatcgagat cgcggcgcaa tcccagcagc
     9541 gtgccggcta ccagcagcag caacgcgagc acgacgatgg tcagcagggc gtggaactga
     9601 tgacgcaacg gcaggttgcg ccagcggctg ctcagggagg cggagccggg cacggactcc
     9661 agggctgtgg acggcacttg cggcagggta gcggtcacga gaattctccc tgtgacgata
     9721 cagaggacgg agcaacggat gggcgtggtt tgcgtgtccg gaattccaga gaaaaatcaa
     9781 attaccacat ggttattgct gtatatattc gtgcgcacct gaacagccat gcaacgaaag
     9841 tggtagttgc cggtacgaaa aatcctgctg accgactctt tcgccaccgt ctgcgannnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggg agagacagcc cggaagccgg
    10021 caacggcttt tcagccggcg gtcgtgatcg cgtcgtttcc gagccgtcgg gccgtggcgt
    10081 agacgcagtg acagcgcccc tcgcgcaggc tgcgttcggc gttggcgagc acggtcagca
    10141 gttcgtcggc cggcagcacc ggattgtcgg ggcactccag cttcgcgagc gtggcgcgct
    10201 gggcggcata ggcatcggcc gctgcctgcg agccatcgac aaaccccgcc tcggcgaatc
    10261 cggcggcgcg caggccggcg gccagggccg cctgcgtctg cagatggctc agcgtgccgt
    10321 cggcgcgcga ccacggcagc gggtgatacg gctcgccgcc gctgccgagg gtccattcgt
    10381 gcagcgccag caccccgccc gggcgcagca gccgtgccgc ctcctgccac agcgcctgcg
    10441 gttcgggcag gtgcagcgcc agatgctgga cccagaccgc atcgaaaccg ccggccggca
    10501 ggccggtggc gctcgcatcg gcgtgcaggt gctgcacctg cgcatccagc ccgacccagg
    10561 ccgtcagttc ggcgccggcc tcgaccagcg tccgggtcag gtcgacgcag accacacggc
    10621 agccgagttc gccggcgagc cagcgcgcgc tgccgccgag tccaccgccg agatcgacca
    10681 tgccggcccc gcgtccgagc ccggcgagac cggccagtgt cgcgaccgcc ttcaggccgc
    10741 cggcgtgaat ctggtcgtag cgggccacgt cgcgccagcc gggccgctcg atgccgtcgg
    10801 cggcgagccg ctgctggatc cactgccgcg gcgtgccgcg gctccagtgc gcgatgacat
    10861 cggcctggtc cgcatccggt gtcggcgcgg aagctccgga acagggcatc gggtggttca
    10921 tggcgggcag gctccatgca gatccgggaa ggcccgaagt ccgggcatcg ggcggccggg
    10981 cgtcgcaccg tatcctgtct gcaagcgtgc cgacccgctg cgtccgctgc tgcatcgatg
    11041 gttgacaatc cggacccgca caccggtgat ggcgattccc gtcgccgccg ccatgaaccc
    11101 gcatgaacga cgcccgcctt gccgatctgc gtgaacaggc cgtccggctt ggatacggcc
    11161 tgcagaccat gctgccggtt tccgccatct ccgcccccgg tctcgcctcg ctcgcggccc
    11221 cgttccgaac gctgtggctg gtctgcaata ccggcgatgc cgtgcagcgg cgctggcgcg
    11281 ccgggcgcga catcgaggcg ctgctcgctg acaacgccgc gaccggcgcc ggttcctgcg
    11341 gcccgctcga tgacttctgc cgcgccgaac tcggcgccct gctcggcaca cgggcgcacc
    11401 gcgtgctgca tccgcatccg gcctcgaccg cgccgctgcc gctgctcgca ctgctcgccg
    11461 cggccggctg gctgcggccg ggaccgttca tgaacacgct gcatgcgcgc tacggcagct
    11521 ggtgggcggt gcgggccgtg atcgcgctcg atgccggcgc cgatgcgccg ggcgaagccc
    11581 tcggcccatc accgtgcctc gactgcagcg ctccgtgcgt ccctgcctgc ccggcggcag
    11641 cggtcggcaa accgggctgg gaggcgcagc gctgtctcgc acagcgcgtt acgatcggca
    11701 gcggatgtgc ccgacgctgc atcgcgcgcg aaagttgtcc ggcaggccgg gaatggcgtt
    11761 acgagccggc cacgatcgcc tatcactacg gtgcatcgca gcaaacactg cagcgctggc
    11821 gtggcgggga gcgcgcctga gcaaccgccc gaggggatcg ccatgctgac cgtcatcgcc
    11881 cgcatcgaag cccgccccga attcgccgcc tgcctgaagc aggccatgct cgcgctgctg
    11941 gccccgaccc gtgccgaacc cggctgcctc gcctacgaac tgctggaaag cagcgacgat
    12001 ccggcgctga tcttcttcca cgaacgctgg gctgaccggg atgccctcga agcccactgg
    12061 cagtcggcgc acctgctcgc ctatcgggac gcgaccaagg gctggatcga ccggcgcgag
    12121 ctcaccgtgc tgcggccggt cgagccttga gcccgcgaaa gccgcgcagc agcatcgggc
    12181 tggctgaatg gacttcgaat gggatgaaac caaggcgctt gcgaacctgc gcaagcatgg
    12241 tgtcagcttc gcggaagctt cgaccttgtt cggtgatccg ctggagctga cgattgccga
    12301 cccggatcac tcgcacggcg agttccggtt cctgagcctc ggtcgcagcc atgccggacg
    12361 tctgctggtc gtggcctata cggaacggga ccaccgcatc cgcatcatca gcgcccgccc
    12421 cgccacttcc cgggagatcc agtgccatga agccggcaga ttctgattct gataccgaca
    12481 ccgaattgcg cgacgagtac gatttttcgg ccgccatccg cggcaggcat cacagcgact
    12541 atcagcgcgg caccaacctc gtgttgctgg acgccgatgt cgcggccgcc tttccggatg
    12601 cggcagccgt caacaacgcc ctgcgcctgc tgctgaggct cgcccggcag caactgccgc
    12661 ccgtccccaa gccttcggaa tgatgcaggt ttgcccggtg acgaacgtca tggtgcgctt
    12721 cgcggctcag gccgcgaacg ccgccccgca ggcaccgctg atcgccgccc aggccccggc
//
LOCUS       DAOQIE010000075        12732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2406_length_12732_cov_9.766112, whole genome shotgun sequence.
ACCESSION   DAOQIE010000075 DAOQIE010000000
VERSION     DAOQIE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2406_length_12732_cov_9.766112"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PK144_06530"
     CDS             <1..194
                     /locus_tag="PK144_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF65417.1"
                     /translation="QYLRTQQPKTLVAWGRHDPIFIAAGAEAFRRDLEQPHIHYLESG
                     HFALEEDVEAIAALIRSTF"
     gene            complement(278..703)
                     /locus_tag="PK144_06535"
     CDS             complement(278..703)
                     /locus_tag="PK144_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF65418.1"
                     /translation="MIVIDTNVISELWKIEPNPGVLAWIDAQAIETLYLSAITVAELR
                     YGLATMPDGRRRTIYQDRLEQEVLPAFTARVLPFDLEASRAYADLMARARAEGKVIGK
                     ADGYIAAIANARGFTVATRDVAPFQVAGLHVINPWQAML"
     gene            complement(700..957)
                     /locus_tag="PK144_06540"
     CDS             complement(700..957)
                     /locus_tag="PK144_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNF65419.1"
                     /translation="MPSITIRNVPDEVHRAIRVRAARHGRSTEAEIRDILEQAAKPEG
                     RLKLGSLLTSIAREAGGLTDAEAGHFDQLRDRTLAEPLRFE"
     gene            1294..>1999
                     /locus_tag="PK144_06545"
     CDS             1294..>1999
                     /locus_tag="PK144_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNF65420.1"
                     /translation="MPVSHLLLAVLVTVLWGWNFVAIKAGLDSLPPLLFTALRFVIAA
                     LPALWLPRPAGSWKWLLLAGGGIGVVQFGCLFTAMDGHISPGLASLVLQMQAFFTVLL
                     ARVIDGTKPRPWQWWGLALAFAGIALIGTDLDASVSAGGLMLILVAALGWASGNLALR
                     RAGAVNMLHFVVWMSLVPPLPLVLASLCFEGPDALSRAWTAIDPTGAAALGYIVVAST
                     WIGYGVWGWLMQRHAVT"
     assembly_gap    2000..2099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2100..2330
                     /locus_tag="PK144_06550"
     CDS             <2100..2330
                     /locus_tag="PK144_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNF65421.1"
                     /translation="TWIGYGVWGWLMQRHAVTLVAPFSLLVPLNGMLAAMLVYGEHYG
                     PLRLVACALVLAGLTLNTFGGRLRWWRPALAS"
     gene            2414..2641
                     /locus_tag="PK144_06555"
     CDS             2414..2641
                     /locus_tag="PK144_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNF65422.1"
                     /translation="MNEFERITQNPAVMGGKPCIRGMRVTVGAIVSQIGAGRCIEEVL
                     ADYPYLEREDVLQALRYAAWRLQEREIILGA"
     gene            2656..3042
                     /locus_tag="PK144_06560"
     CDS             2656..3042
                     /locus_tag="PK144_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNF65423.1"
                     /translation="MKLLIDMNLTPRWVAYLHDAGFAAEHWSMCGPVDASDASIMAFA
                     QTGDYVVLTHDLDFGAILAATHGQKPSVIQLRGEMPIPETIGSLLVQALEQLQSELVA
                     GALLTIDLKRARLRLLPLLLDNSGGR"
     gene            complement(3078..6248)
                     /locus_tag="PK144_06565"
     CDS             complement(3078..6248)
                     /locus_tag="PK144_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF65424.1"
                     /translation="MNLSGFFISRPIFAGVLSALILIAGLISLPVLPISEYPDVVPPT
                     VVVRAQYPGANPVTIGETVASPLEQQINGVEGMLYQSSQATGDGTLTLTITFALGTDV
                     DKAQVLVQNRVAQALPKLPAEVQRLGVTTNKASPDLTMVVHLLSPDQRYDMLYLSNYA
                     ALRVKDELARLDGVGDVQLFGAGQFAMRVWLDPERLASRGLGAGDVLRAIREQNVQVV
                     AGSLGAPPAPNAADFQIAVDAGGRLASEEQFGNIVVKTGTDGQLVRLRELGRVELGAD
                     SYALRSLLDNQPAVAMPIFQRPGSNAIAISDAVRAKMAELAKAFPQGVEYRIVYDPTV
                     FVRGSIEAVVHTLLEAVALVVIVVIVFLQTWRASIIPLLAVPVSLVGTFAVMHLFGFS
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNIENGLKPIDATRQAMREVTGPIVATALV
                     LCAVFVPTAFISGLTGQFYRQFALTIAISTVISAFNSLTLSPALAARLLPEHGAAPDR
                     LTRLLNAALGWFFRPFNRGFARAAHGYTGLVRRTLRLSGIVLVLYAGLVALTYAGFTR
                     TPTGFVPGQDKQYLVAFAQLPDAATLDRTEDVIRRMSEIALAEPGALNAVAFPGLSIN
                     GFTNSPNSGIVFVTLKPFEERRSAELSAGAIAAKLNGKFAAIQDAFIAIFPPPPVMGL
                     GTIGGFRLQIEDRAGLGYAELFRQTQNLIAKSHERPELAGLLSSFQINVPQLKTDIDR
                     DKALAAGVAPADVFETLQVYLGSVYANDFNRFGRTYPVYVQAGAERRLEPADVLRLQT
                     RNRDGDMIPLGAFVSVTPGSGPDRVSHYNGYPTAEINGAAAPGFSSGQAQAAMEAIAR
                     AELPDGMSYEWTELTYQQLLAGDTTLYVYPLVVLLVFLVLAAQYESLRLPLAVILIVP
                     MCLLSALAGVNLAGSDNNVFTQIGLIVLVGLACKNAILIVEFAREREHEGEDVVQAVL
                     DACRLRLRPILMTSIAFIMGVVPLVLSSGAGAEMRHAMGVAVFAGMLGVTFFGLLLTP
                     VFYAVLRRGPARRERAAVTAVPAAGH"
     gene            complement(6393..7649)
                     /locus_tag="PK144_06570"
     CDS             complement(6393..7649)
                     /locus_tag="PK144_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF65425.1"
                     /translation="MSRHTPNVRLARPLILSTPVAALVLALGLALSGCEDSVAVTPPP
                     APQAAPVTVAEVLSKPLRDWHEFTGRLEAESTVELRPRVSGYVDRVAFTEGARVKAGE
                     LLFAIDARPFRAEVARLAAERDRAAAELKLAQGNAARGQRLLPQRAIAQEEADRLTTL
                     AVTAQSALAASEAALTRARLDLEFTEVRAPVSGHVSRALITAGNLVTPESVLTTVVAD
                     ERIHAYFDVDEQTFLRVGNDTGNARVQMGLASDSGYPHAGRIDFVDNRVDARTGTIRV
                     RALFDNADGRYTPGLYARVRLLGPELRDTVLIEDRAIGTDLDRRFVLVLGADGTVAYR
                     AVRLGPQIDGLRVVTDGLAAGDTIVVNGLQRVRPGAKVAATHVSMQLDDGGATLKQIA
                     GDDGAPDDRPAAHPAASRGPTRIAVR"
     assembly_gap    7796..7895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7896..8492
                     /locus_tag="PK144_06575"
     CDS             7896..8492
                     /locus_tag="PK144_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF65426.1"
                     /translation="MICGTAKKAGRVRDRIFATARELFSRHGVRGVGVDAIACEAGTN
                     KMSFYRNFASKDDLVAECLSASESEFWAWWDAQTQPHAGDPRRQIEALFDGFVTKVCT
                     DQDRTRGCALANAVVEITEEDHPGRQVAVAFKAEVRRRLQLWAHELGAREPEMLGDAL
                     LLLMDGCYLSRLAFGDKGPVRSAGSAARALIAAQLAPA"
     gene            8585..10084
                     /locus_tag="PK144_06580"
     CDS             8585..10084
                     /locus_tag="PK144_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF65427.1"
                     /translation="MSTVFQPSWADLAPDAVILSTEDGTVLQWAGGAETLYGYTHAAA
                     LGATIDELIVVPEDQAEQTQRLETVLAHGTLTFETIRRCRDGTLIYVDVSARCIATGN
                     DERGISLLWIEKDVTALRVRRDGRLLSARYHDLLESTPDGIVLADRTGHVVIANSHAE
                     HLFGYAPGELDGQPVEMLLPERLRSMHVTHRAHYRAQPHARPMGAGLELAARRRDGSE
                     FPVEISLSPLATEVGPMTMSAIRDVTERKRFEKALQEKNHELAAANQAKTQFLASMSH
                     ELRTPLNAIIGFTGALLMKLSGPLTSVQEEQLKLVQSSGKHLLALIGDLLDLARIEAG
                     RIDLTPEPLDAVAITNEVVASLRPQAVHKGLALNVAHEAPVLALHADRRALTQILINL
                     IGNAIKFTERGHVHVHSERAFDSDGRALVRIAIEDTGLGIRPEEQAQLFEAFARGLVA
                     KRAGIEGTGLGLHLSRKLTLLLGGNLSLRSEFGSGSTFTLELPAGPAAG"
     gene            10160..10546
                     /locus_tag="PK144_06585"
     CDS             10160..10546
                     /locus_tag="PK144_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65428.1"
                     /translation="MSASILIVEDHGPSRQLLDFLLRARGYRTRLAGNGTEAMTTLER
                     EPIDLVLCDLHMPVMDGYVFIHRLRADARFEALPVLAVTASTLTGDRDRVLAAGFTGF
                     FSKPIDPEHFVEHMEAFLPPGQRAAG"
     gene            complement(10583..11074)
                     /locus_tag="PK144_06590"
     CDS             complement(10583..11074)
                     /locus_tag="PK144_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF65429.1"
                     /translation="MDWQEICASPLLRDLPFKIETDALGRIVMSPASNRHGLYQSRIA
                     VLLSRLLPGGESQTECSVATADGVKVAGVVWRSDAFAARHGDATPYPLAPEIVVEIVS
                     PSNTVAELLRKKELYFARGAQEFWLCAGDGALRFFNNHSELAASALAPEFPATVARRP
                     DAH"
     gene            complement(11204..11788)
                     /locus_tag="PK144_06595"
     CDS             complement(11204..11788)
                     /locus_tag="PK144_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNF65430.1"
                     /translation="MMSRKVLMIVGDFAEDYETMVPFQTLLTLGYTVHAVCPERAAGE
                     RIRTAIHDFEGDQTYSEKPGHGFTLNASFADVSAADYDALLIPGGRAPEYLRMNARVL
                     ELVRAFDDAQKPIAAVCHGAQLLAAAGILKGRRVSAYPACAAEVKLAGAEYADIAIDA
                     AVTDGHLVTAPAWPAHPAWLAQFIARLGTTITHG"
     gene            12037..12600
                     /gene="ahpC"
                     /locus_tag="PK144_06600"
     CDS             12037..12600
                     /gene="ahpC"
                     /locus_tag="PK144_06600"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNF65431.1"
                     /translation="MSLINTEVKPFKAQAYKDGKFVTVTEADLKGKWSVVCFYPADFT
                     FVCPTELEDLAAYYEEFQSRGVEIYSVSTDTHFAHKAWHDTSEAIGKVRYTMIGDPTG
                     AITRNFDVMIEDAGLALRGTFLINPDGVIKLCEIHDNGIGRDAGELLRKIKAAQYVHT
                     HPGEVCPAKWKEGEKTLTPSLDLVGKI"
BASE COUNT         1969 a         4327 c         4319 g         1917 t
ORIGIN      
        1 agcagtacct gcgcacgcag cagccgaaga cgctggtcgc ctggggccgg cacgacccga
       61 tcttcatcgc cgccggcgcc gaagccttcc ggcgcgacct cgagcagccg cacatccact
      121 acctcgaatc cggccacttc gcgctggaag aggacgtgga ggcgatcgcg gcgctgatcc
      181 gcagcacgtt ctgaagctcg ctgccgcctc tggtgcatgg caggcggcgc ttacgagcgc
      241 agccgcaggc gtaatcgggc gcagttccat gctgctgcta cagcatcgcc tgccatggat
      301 tgatgacgtg gaggccggcc acctggaacg gcgccacgtc gcgtgtggca acggtgaagc
      361 cgcgagcgtt ggcaatcgcg gcgatgtagc cgtctgcctt gccgatgacc ttgccctcgg
      421 ccctcgcccg cgccatcagg tctgcataag cccgggaagc ctcgagatcg aacgggagaa
      481 cacgagccgt aaaggccggc aatacctcct gttccagacg atcctgatag atcgtgcggc
      541 gccggccgtc gggcatcgtg gcaaggccat agcgcagctc ggccacggtg atggcggaca
      601 gatagagcgt ttcaatggcc tgtgcgtcga tccacgccag cacgccggga ttcggttcga
      661 tcttccacaa ctcggaaatc acgttggtat cgatgacgat cattcaaacc tcagcggctc
      721 ggccagcgtc ctgtcgcgga gctggtcgaa gtgcccggct tcggcatcgg tcagaccgcc
      781 ggcctcgcgg gcgatggaag tcagcagcga accgagtttc aggcggcctt cgggcttcgc
      841 cgcctgctcc aggatgtcgc ggatttcagc ttcagtgctg cgaccgtgcc tggctgcacg
      901 aacccggatc gcccggtgta cctcatccgg gacgttgcga atggtgatgg atggcatggc
      961 tttctccgtc atgttttgat tgcagtgatt gcaatatatt aaattgcaat cacttctgcc
     1021 atcagcaaaa tatcgatcag gatcgagtgc agtccgcttt acccggtaca caccggggac
     1081 gaaggctgcg tgtaacggtc aagttccggc ggtcgtttgc gccatcacgg tgcccgcgtc
     1141 cggaaccggc atcctgcaga agcgatgcag tgatccggca atccggcgcc tcaacccccg
     1201 gcaggactga atgactgccc tgcaccggca gggctgccac cgccctcgat gtcatgaaat
     1261 cctgcgcgcc ccctgccgcc ggacgccccc gccatgcccg tctcccacct gctgctcgcc
     1321 gtgctggtca cggtgctctg gggctggaac ttcgtcgcga tcaaggccgg gctcgacagc
     1381 ctgccgccgc tgctgttcac ggcgctgcgc ttcgtgatcg cggcgctgcc ggcgctgtgg
     1441 ctgccgcgcc cggccggttc gtggaagtgg ctgctgctcg ccggcggcgg catcggcgtc
     1501 gtgcagttcg gctgcctgtt cacggcgatg gacggacaca tctcgccggg gctggcgtcg
     1561 ctggtgctgc agatgcaggc cttcttcacg gtgctgctgg cgcgcgtcat cgatggcacg
     1621 aagccgcggc cgtggcagtg gtgggggctg gcactggcgt tcgccggcat cgcgctgatc
     1681 ggcaccgacc tcgatgccag cgtcagcgcc ggcggcctca tgctgatcct ggttgccgcg
     1741 cttggctggg cgagcggcaa tctggcgctg cgccgggccg gggcggtcaa catgctgcac
     1801 ttcgtggtct ggatgagcct ggtgccgccg ctgccgctgg tgctggcctc gctgtgcttc
     1861 gaaggccccg atgcgctgtc gcgggcctgg acggcgatcg acccgaccgg cgccgccgcg
     1921 ctcggctaca tcgtcgtcgc ctccacctgg atcggttacg gcgtctgggg ctggctgatg
     1981 cagcgccacg cggtgacgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2101 cctggatcgg ttacggcgtc tggggctggc tgatgcagcg ccacgcggtg acgctggtcg
     2161 cgccgttctc gctgctggtg ccgctgaacg gcatgctggc ggcgatgctg gtctacggcg
     2221 agcactacgg gccgctgcgc ctcgtcgcct gcgcgctggt gctggcgggg ctgacgctga
     2281 acaccttcgg cgggcggctg cggtggtggc ggccggcgct ggcaagctga ccggatactg
     2341 catgcgctga tcctgaccgg cctgcgatac ttccatcgtg cgcaacgccg cagagacccg
     2401 gaggatcgac gccatgaacg agttcgaacg catcacccag aacccggcgg tcatgggcgg
     2461 caaaccgtgc attcgtggca tgcgcgtgac cgtgggtgca atcgtcagcc agatcggtgc
     2521 gggccggtgc atcgaggagg tgctggccga ctatccctat ctggaacgcg aggatgtcct
     2581 gcaggcgctg cgctatgccg cctggcgtct gcaggaacgc gaaatcattc tcggcgccta
     2641 gcgtgacgac caggcatgaa actgctgatc gacatgaacc tgacgccgcg ctgggtggct
     2701 tacctgcacg acgcagggtt tgcagccgag cactggtcaa tgtgtggtcc ggtcgatgcg
     2761 tccgatgcaa gcatcatggc ttttgcacag acgggtgatt acgtcgtgct gacacacgat
     2821 ctcgacttcg gcgccatcct cgccgcaacg cacggccaaa agccaagcgt catccagctc
     2881 agaggcgaga tgccgattcc ggaaaccatc ggcagcctgc tcgttcaggc actggaacaa
     2941 ctgcagtccg aactggttgc cggtgccttg ctgaccatcg atctgaaacg ggcacgactg
     3001 cgtttactgc cactgctgct ggataattcc ggcgggcgct gaccggaacg gcccgtcgcc
     3061 cgccggaagg ctccggctca gtgccccgcc gccggcaccg ccgtcaccgc cgcccgctcc
     3121 cgccgcgccg gcccgcggcg cagcaccgcg tagaacaccg gcgtcagcag caggccgaag
     3181 aaggtcacgc cgagcatgcc ggcgaacacc gccacgccca tcgcgtgacg catctcggcc
     3241 ccggcgccgc tcgagagcac cagcggcacc acgcccatga tgaacgcgat cgaggtcatc
     3301 aggatcgggc gcaggcgcag gcggcaggcg tcgagcacgg cctggaccac gtcctcgccc
     3361 tcgtgctcgc gttcgcgcgc gaactcgacg atcaggatcg cgttcttgca ggcgaggccg
     3421 accagcacga tcaggccgat ctgcgtgaac acgttgttgt cgctgccggc gagattgacg
     3481 ccggcgagcg ccgacagcag gcacatcggc acgatcagga tcaccgccag cggcaggcgc
     3541 aggctctcgt actgcgcggc gagcaccagg aacaccagca gtacgaccag cggatacacg
     3601 tacagcgtcg tgtcgccggc gagcagctgc tgataggtca gctcggtcca ttcgtagctc
     3661 atgccgtccg gcagctcggc acgcgcgatc gcctccatcg ccgcctgcgc ctggccgctg
     3721 ctgaagcccg gtgccgcggc gccgttgatc tcggcggtcg ggtagccgtt gtagtggctg
     3781 acgcgatccg gccccgagcc gggcgtgacg ctgacgaagg caccgagcgg gatcatgtcg
     3841 ccgtcgcggt tgcgcgtctg caggcgcagc acgtcggcgg gctccagccg gcgttcggcg
     3901 ccggcctgca cgtagaccgg gtaggtgcgc ccgaagcggt tgaagtcgtt ggcgtagacc
     3961 gagccgagat acacctgcag cgtctcgaac acgtccgccg gcgcgacccc ggcggccagc
     4021 gccttgtcgc ggtcgatgtc ggtcttcaac tgcggcacgt tgatctggaa gctcgacagc
     4081 aggccggcga gttccgggcg ttcgtggctc tttgcaatga ggttctgcgt ctggcggaac
     4141 agctcggcat agccgaggcc ggcgcgatcc tcgatctgca gacggaagcc gccgatcgtg
     4201 ccgaggccca tgaccggcgg cggcgggaag atggcgatga aggcgtcctg gatggcggcg
     4261 aacttgccgt tgagcttggc ggcgatcgcc ccggccgaca gctccgccga gcggcgttcc
     4321 tcgaagggtt tcagcgtcac gaacacgatg ccgctgttcg ggctgttggt gaagccgttg
     4381 atcgacaggc ccgggaaagc cacggcgttc agcgcgcccg gctcggcaag cgcgatctcg
     4441 ctcatgcgcc ggatcacgtc ttcggtgcga tcgagggtgg cggcgtccgg cagctgcgcg
     4501 aaggcgacca gatactgctt gtcctggccc ggcacgaaac cggtcggcgt gcgcgtgaag
     4561 ccggcatacg tcagcgcgac cagcccggcg tacagcacca gcacgatgcc cgacagccgc
     4621 agcgtgcggc gcacgaggcc ggtgtagccg tgcgcggcgc gcgcgaagcc gcggttgaac
     4681 ggccggaaga accagccgag cgcggcgttg agcagcctgg tgagccggtc gggcgccgcg
     4741 ccgtgctcgg gcagcaggcg cgcagcgagc gccggcgaca gcgtcagcga gttgaaggcc
     4801 gagatcacgg tgctgatggc gatggtcagc gcgaactgcc ggtagaactg cccggtcagg
     4861 ccgctgatga aggccgtcgg cacgaacacc gcgcagagca ccagcgcggt cgcgacgatc
     4921 gggccggtga cctcgcgcat cgcctggcgc gtggcgtcga tgggcttcag gccgttctcg
     4981 atgttgcgct cgacgttctc gaccacgacg atcgcgtcat cgacgacgat gccgatcgcc
     5041 agcaccagtc cgaacaggct cagcgcgttc agcgagaagc cgaacaggtg catgaccgcg
     5101 aaggtgccga ccagcgacac cggcaccgcc agcagcggga tgatcgacgc gcgccaggtc
     5161 tgcaggaaca cgatcacgac gatgacgacg agcgccacgg cttcgagcag cgtgtgcacg
     5221 acggcctcga tcgagccgcg cacgaacacg gtcgggtcgt agacgatgcg gtactcgacg
     5281 ccctgcggga aggctttcgc gagttcggcc atcttggcgc gcacggcgtc ggagatggcg
     5341 atggcgttgc tgccggggcg ctggaagatc ggcatcgcca ccgccggctg gttgtcgagc
     5401 agcgagcgca gcgcgtagga atcggcgccg agctcgacgc gccccagctc gcgcaggcgc
     5461 acgagctggc cgtcggtgcc ggtcttgacg acgatgttgc cgaactgctc ctcgctcgcg
     5521 aggcgcccgc cggcatcgac ggcgatctgg aagtcggcgg cgttcggggc cggcggcgcg
     5581 ccgaggctgc cggcgaccac ctgcacgttc tgctcgcgga tcgcgcgcag cacgtcgccg
     5641 gcgccgaggc cccggctcgc gagccgctcc ggatcgagcc agacgcgcat cgcgaactgg
     5701 ccggcgccga agagttgcac gtcaccgacg ccatcgaggc gggcgagctc gtccttgacc
     5761 cgcagcgccg cgtagttcga caggtacagc atgtcgtaac gctgatcggg cgagagcagg
     5821 tgcacgacca tggtcaggtc gggcgaggcc ttgttggtgg tcacgccgag gcgctgcacc
     5881 tcggccggca gcttcggcag cgcctgcgcg acgcggttct gcaccagcac ctgcgccttg
     5941 tcgacatcgg tgccgagcgc aaacgtgatg gtcagcgtca gcgtgccgtc accggtcgcc
     6001 tgcgaggact ggtacagcat gccctcgacg ccgttgatct gctgctccag cgggcttgcg
     6061 accgtctcgc cgatcgtgac cgggttggcg ccggggtatt gcgcgcgcac gaccacggtc
     6121 ggcggcacca cgtccggata ttccgaaatc ggcagcaccg gcagcgagat caggccggcg
     6181 atcaggatca gcgccgacag cacgccggcg aagatcgggc gggagatgaa gaatccggac
     6241 aggttcatga gggctccggc ttcggttgca cctgcgtgcg ggcacaggcg gggagatccg
     6301 gcggcgcgcg ggcgcagcgc cgggctgggg cgttcgggga tccggggaac cgcaggggag
     6361 tcacggccgc cgcgccggcg gccgttgcgg cttcagcgca ccgcgatgcg cgtcggtccg
     6421 cggctggcgg ccggatgcgc ggccggcctg tcgtccggcg cgccgtcatc gccggcgatc
     6481 tgcttcagcg tcgcgccgcc gtcatcgagc tgcatcgaca cgtgcgttgc cgcgaccttc
     6541 gcgcccggac gcacgcgctg caggccgttg acgacgatgg tgtcgccggc cgcgaggccg
     6601 tcggtgacca cgcgcaggcc gtcgatctgc gggccgaggc gcacggcgcg gtaggcgacc
     6661 gtgccgtcgg cgccgagcac cagcacgaag cggcggtcga ggtcggtgcc gatggcgcga
     6721 tcctcgatca gcacggtgtc gcgcagctcg ggcccgagca ggcgcacgcg cgcatacagg
     6781 cccggcgtgt agcggccgtc agcgttgtcg aacagcgcgc gcacgcggat cgtgccggtg
     6841 cgggcatcga cgcggttgtc gacgaagtcg atgcgtccgg cgtgcggata gccgctgtcg
     6901 ctggcaagcc ccatctgcac gcgggcgttg ccggtgtcgt tgccgacgcg caggaaggtc
     6961 tgctcatcga catcgaagta ggcatggatg cgctcatcgg cgacgacggt ggtcagcacg
     7021 ctctcgggcg tgaccaggtt gccggcggtg atcagcgccc gcgacacgtg gccggaaacc
     7081 ggcgcgcgca cctcggtgaa ttcgagatca agcctggcgc gcgtcagcgc cgcctcgctg
     7141 gcggcgagcg cgctctgcgc ggtgacggcg agcgtcgtca ggcggtcggc ttcttcctgc
     7201 gcgatcgcgc gctgcggcag caggcgctgg ccgcgcgcgg cgttgccctg cgcgagcttc
     7261 agctccgccg cggcgcgatc gcgttcggcc gcaagccgcg cgacctcggc ccggaacggc
     7321 cgcgcgtcga tcgcgaacag cagctcgccg gccttgacgc gcgcgccctc ggtgaaggcg
     7381 acgcgatcga cgtagccgct gacgcgcggg cgcagctcga ccgtgctctc ggcttcgagc
     7441 cggccggtga actcgtgcca gtcgcgcagc ggcttgctca gcacctcggc gaccgtcacc
     7501 ggcgcggcct gcggagccgg cggcggcgtg acggcgacgc tgtcctcgca accggacagc
     7561 gccaggccga gcgccagcac cagcgcggcg accggggtgg acaggatcag gggacgggca
     7621 agcctgacgt tcggtgtgtg gcgggacatg gcggcggact cccgggggcg gagatcagga
     7681 acgggcagcg gttcagcgcg tatccggcgg gcatccggtg cgatccggcc catccttgac
     7741 gggaactcgc gcgggaccga tgctgtacaa gccgttaccg accggtaaca tgtgcnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgat ctgcggcacg gcgaaaaagg
     7921 ccgggcgcgt gcgcgaccgc atcttcgcga ccgcccgcga gctgttctcg cgccacggcg
     7981 tgcgcggcgt cggcgtcgat gccatcgcct gcgaggccgg caccaacaag atgagcttct
     8041 accggaactt cgcatccaag gacgatctgg tcgccgagtg cctgagcgcc tcggaaagcg
     8101 agttctgggc ctggtgggac gcgcagacgc agccgcacgc cggcgacccg cgccgccaga
     8161 tcgaggcgct gttcgatggc ttcgtcacca aggtctgcac cgatcaggac cgcacgcgcg
     8221 gctgcgcgct cgccaacgcc gtcgtcgaga tcaccgagga ggatcacccc ggccgtcagg
     8281 tggccgtggc cttcaaggcc gaagtgcgcc gccgcctgca gctctgggcg cacgagctcg
     8341 gtgcgcgcga gcccgagatg ctcggcgacg cgctgctgct gctgatggac ggctgctatc
     8401 tgtcgcggct cgccttcggc gacaaggggc cggtgcgcag cgccggcagc gcggcgcggg
     8461 cgctgatcgc cgcgcaactc gcacccgcct gaagccggtg ccgcgcgggc cggccgtatt
     8521 gttttcagcg tttccccgcg tatggtgcgg ccatccaccc gcccggcctg cggagaccag
     8581 cgacttgagc accgttttcc agccttcctg ggccgatctc gcgcccgatg ccgtgatcct
     8641 cagcaccgag gacggcaccg tcctgcagtg ggccggcggc gccgagacgc tttacggcta
     8701 cacgcacgcc gccgcgctcg gcgccacgat cgacgagctg atcgtcgtgc ccgaggatca
     8761 ggccgagcag acgcagcgcc tcgaaacggt gctcgcccac ggcacgctga ccttcgagac
     8821 catccgccgc tgccgcgacg gcacgctgat ctacgtcgat gtctcggccc gctgcatcgc
     8881 caccggcaac gacgagcgcg gcatcagcct gctgtggatc gagaaggacg tgacggctct
     8941 gcgcgtgcgc cgcgacggcc gtctgctcag cgcccgctac cacgacctgc tcgaatcgac
     9001 accggacggc atcgtgctcg ccgatcgcac cggccacgtg gtcatcgcca acagccacgc
     9061 cgaacacctg ttcggctacg cacccggcga gctcgacggc cagccggtcg agatgctgct
     9121 gcccgagcgc ctgcgctcga tgcacgtcac gcaccgtgcg cattaccgcg cgcagccgca
     9181 cgcgcgaccg atgggcgccg gcctcgaact ggccgcgcgc cggcgcgacg gcagcgaatt
     9241 cccggtcgag atcagcctga gcccgcttgc caccgaggtc gggccgatga cgatgtcggc
     9301 gatccgcgac gtcaccgagc gcaagcgctt cgaaaaggcc ctgcaggaga agaaccacga
     9361 gctcgccgcc gccaaccagg ccaagaccca gtttctggcc tcgatgagcc acgagctgcg
     9421 cacgccgctg aacgccatca tcggcttcac cggcgcgctg ctgatgaagc tctccggccc
     9481 gctgaccagc gtgcaggaag aacagctcaa gctcgtgcag tcgagcggca agcacctgct
     9541 ggcactgatc ggtgacctgc tcgacctggc gcgcatcgag gccgggcgca tcgacctgac
     9601 gccggagccg cttgatgccg tcgccatcac caacgaggtc gtcgcctcgc tgcgcccgca
     9661 ggccgtgcac aaggggctgg cgctgaacgt cgcgcacgag gcgccggtgc tggcgctgca
     9721 cgccgatcgc cgggccctga cgcagatcct gatcaacctg atcggcaacg ccatcaagtt
     9781 caccgagcgc ggccacgtgc acgtgcacag cgaacgcgcc ttcgacagcg acggccgggc
     9841 gctcgtgcgc atcgcgatcg aggacaccgg cctcggcatc cgccccgagg aacaggcgca
     9901 gctgttcgag gccttcgcgc gcggcctggt cgccaagcgc gccggcatcg agggcaccgg
     9961 cctcggcctg cacctgagcc gcaagctgac gctgctgctc ggcggcaacc tgtcgctgcg
    10021 cagcgaattc ggcagcggca gcaccttcac gctggaactg ccggccggtc ccgccgccgg
    10081 ctgaggccgg cggatgccac gatcaccggg ccgatcgggt aacgtaggct gcttcccgtt
    10141 ctccaccccg gtccgcgcga tgagtgccag catcctgatc gtcgaagacc acggtccgag
    10201 ccgccagctg ctcgacttcc tgctgcgcgc acgcggctat cgcacgcgcc tcgccggcaa
    10261 cggtaccgag gcgatgacga cgctcgaacg cgagccgatc gacctcgtgc tctgcgacct
    10321 gcacatgccg gtcatggacg gctacgtgtt catccaccgg ctgcgcgccg atgcgcgctt
    10381 cgaggccctg ccggtgctcg ccgtcaccgc ctcgacgctg accggcgacc gcgaccgcgt
    10441 gctggccgcc ggcttcaccg gcttcttctc gaagccgatc gatccggagc atttcgtcga
    10501 gcacatggaa gccttcctgc cgcccgggca gcgcgccgcc ggctgagagc gagccggact
    10561 tgccacgtgg caagcccgtg cttcagtgcg cgtccggccg gcgcgcgacc gtggccggga
    10621 actccggcgc cagcgcactc gccgccagct cgctgtgatt gttgaagaag cgcagcgcgc
    10681 cgtccccggc gcacagccag aactcctgcg cgccgcgtgc gaagtacagc tccttcttgc
    10741 gcagcagttc ggcgaccgtg ttcgacggcg agacgatctc gacgacgatc tcgggggcca
    10801 gcggataagg cgtggcatcg ccgtgccggg ccgcaaaggc atcgctgcgc cagaccacgc
    10861 cggccacctt gacgccatcg gcggtcgcga cgctgcattc ggtctgactc tcgccgcccg
    10921 gcagcagccg gctcagcagg acggcgatcc gggactgata cagcccgtgg cggttgctcg
    10981 ccggactcat gacgatcctc ccgagcgcgt cggtctcgat cttgaacggc agatcccgca
    11041 acagcggtga ggcgcagatt tcctgccagt ccatcgcgtg ctccttctgg aagggggccg
    11101 gcgcaccggt cacgcgtgcc tgcgtggttg attcctctct cctcaacccc tcccccgcga
    11161 gggggagaag ccgaaaagac ggcgtgttca gcaaagaccg gtatcagccg tgcgtgatcg
    11221 tcgtgccgag ccgcgcgatg aactgcgcga gccaggccgg atgcgccggc caggccgggg
    11281 cggtgaccag atggccgtcg gtgacggcgg cgtcgatggc gatgtcggcg tactcggcgc
    11341 cggcgagctt cacctcggcc gcgcaggccg gatacgccga cacccgccgg cccttcagga
    11401 tgccggcggc ggccagcagc tgcgcgccgt ggcaaacggc ggcgatcggt ttctgcgcat
    11461 cgtcgaaggc gcgcaccagc tccagcacgc gcgcgttcat gcgcaggtac tcgggcgcgc
    11521 gtccgcccgg gatcagcagc gcgtcgtagt cggcggcgct cacgtcggca aagctcgcgt
    11581 tcagcgtgaa gccgtgaccg ggcttttcgc tgtaggtctg gtcgccctcg aaatcatgaa
    11641 tcgcggtcct gatgcgctcg ccggcggcac gctccgggca gacggcgtgc accgtgtagc
    11701 cgagcgtcag cagcgtctgg aacggcacca tggtttcgta gtcctcggcg aaatcgccga
    11761 cgatcatcag cactttccgg ctcatcacgt actcctcggg acgggccgcc gcagcggccg
    11821 gggtggggaa acgatgcctg ggcggcgatg cggatcgccc cctgcggcgt agcctgcggg
    11881 cgcggtgatt tccatgaatt cagggtggat atttcgtcat cctgtcgata gcaatactca
    11941 atcgacccat tgaccacaat caatttcatc tacgtcgata cgcgccgtaa tctgcggtcg
    12001 tcccttccga acatccccct gagcgaggca agcacgatgt ccctgatcaa taccgaagtg
    12061 aagccgttca aggcccaggc gtacaaggac ggcaagttcg tcaccgtcac cgaggccgat
    12121 ctgaagggca agtggtcggt ggtctgcttc tacccggctg acttcacctt cgtctgcccg
    12181 accgagctgg aagatctggc tgcgtactac gaggagttcc agtcgcgcgg cgtcgaaatc
    12241 tacagcgtct cgaccgacac gcacttcgcg cacaaggcct ggcacgacac ctccgaggcg
    12301 atcggcaagg tccgctacac gatgatcggc gacccgaccg gcgcgatcac ccgcaacttc
    12361 gacgtgatga tcgaggacgc cgggctggcg ctgcgcggca ccttcctgat caacccggac
    12421 ggcgtgatca agctctgcga gatccacgac aacggcatcg gccgtgacgc cggcgagctg
    12481 ctgcgcaaga tcaaggccgc gcagtacgtg cacacccacc cgggcgaggt ctgcccggcc
    12541 aagtggaagg aaggcgagaa gacgctgacc ccgtcgctcg acctcgtcgg caagatctga
    12601 agccttcgtc tgcctcttca gcggcgcgct gccggacacc ccggcggcgg ccgccccgac
    12661 cggggcccgc gcagcttccg gaccgatcgt ccggctgcgc gggcacgcac accacgccgc
    12721 cacccatgcc ac
//
LOCUS       DAOQIE010000076        12690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2417_length_12690_cov_7.429838, whole genome shotgun sequence.
ACCESSION   DAOQIE010000076 DAOQIE010000000
VERSION     DAOQIE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2417_length_12690_cov_7.429838"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /gene="flhF"
                     /locus_tag="PK144_06605"
     CDS             <1..1072
                     /gene="flhF"
                     /locus_tag="PK144_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNF65432.1"
                     /translation="VAPLRAPVEPLLREARPAASRSAGVAAAAAPARQESGARHNVTW
                     AQDPVITQMQTEISALRQLLEQQLTSIAWGREGSRQPRRFELLERLLKFGLDAELCYE
                     LCDAALSEPDNERAWQSALGQLHRGIVTMDDDPLDRGGAIALVGPTGVGKTTTVAKLA
                     ARYALRHGPRKVGLITTDGYRIGAYDQLRTYGILLDLPVRVAGDAPELRQALSDFSDK
                     SLVLIDTAGMGLRDVRLAQQIGMLQTAPAIHNYLVLPANGHPAALGESVQAFAGVQLT
                     GGILSKLDEAARIAGVLSLLYRTGLPLAWFTNGQRVPEDIVSARAETLMATALDQQLD
                     DCGIADDAMLVTWGRRIVNACQ"
     gene            1077..1931
                     /locus_tag="PK144_06610"
     CDS             1077..1931
                     /locus_tag="PK144_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNF65433.1"
                     /translation="MRPEQGSAPRRAAPQNPVRVICVASGKGGVGKTSTTINLAVALA
                     TLRRRVLILDADLGLANVDVLLGLHPVWNLAHVLAGERTLEEILLDGPHGIRIVPASS
                     GIKRMAELSPVEHAGIISAFSELGDAFDVLLVDCAAGISDSVVTFARAAQEVVVVVCN
                     EPASITDAYALIKLLSRDYGVDHLHVLANRVAGHREGVDLYTKLVKVTDRFLDVTLSH
                     IGSIPEDAQLRRAIQMQQAVVDAFPSARASASFLRLAENIERWPLPAGPSGHLQFFLE
                     RLVGAGVR"
     gene            1970..2692
                     /locus_tag="PK144_06615"
     CDS             1970..2692
                     /locus_tag="PK144_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNF65434.1"
                     /translation="MLRASCPYAPARPGLDELVARHAPQVKRIACHLLARLPASVQID
                     DLIQAGMLGLIEAARRYDASQGASFATFAEQRIRGAMLDEIRRGDWTPRSVHRRSREV
                     AAAIRAIETETGREAQDTDVALRLGLSLPEYHDTLTELRGQKLVSLDDDEHGEDEGEG
                     LLAQLASTEAGPEEETAREQLLGAVSAQIDALPEREKLVLALYYDEGLNLKEIGAVLG
                     VSESRVCQIHAQAVARLRSRLR"
     gene            2716..3459
                     /locus_tag="PK144_06620"
     CDS             2716..3459
                     /locus_tag="PK144_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNF65435.1"
                     /translation="MDILSLAGIVLALVAIIGGHLIEGGSLVSLFNPAAFVIVVLGTI
                     AASLLGTPMTVFRGALRRLRWVFVPPPATHEELIEKIVGWSQIARRAGLLGLEDIIER
                     ETEPFTRKALQMLVDGADAETIRHAMEMELAAREDFELRAARLYESMGGYSPTIGILG
                     AVLGLIHVMENLADPSKLGSGIAAAFVATVYGVGFANLVLLPIANKLKSVINRQAQLA
                     EMVIEGVLAIATGENPRNIEMRLRGFIHE"
     gene            3541..4506
                     /gene="motD"
                     /locus_tag="PK144_06625"
     CDS             3541..4506
                     /gene="motD"
                     /locus_tag="PK144_06625"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNF65436.1"
                     /translation="MRHHRRADDHENHDRWLVSYADFITLLFAFFVVMYAISSVNAGK
                     YRVLSENLYSAFRSPISGGALPIGSEPQALSDPQDGRKPNLIAPDLRPLPTPPMRPDQ
                     RPVLDRSTGPDRQTPPLARPVPENAPHAEQPQPGAPPADASQQQLDAVAGALRNAMTD
                     MLQSQQMEVRREKHWVELEIKDNLLFPSGSAQINNQAQDPLARIAGVLKNLPNRVQVE
                     GYTDNVPIRNAVYPSNWELSAARAASVVEVLAHAGVDAQRMAAIGYGEHRPVADNASA
                     DGRLRNRRVVMVVLNQPDPRADVAVDPDAAGTNGPRATDTAGTAP"
     gene            4503..5027
                     /locus_tag="PK144_06630"
     CDS             4503..5027
                     /locus_tag="PK144_06630"
                     /inference="COORDINATES: protein motif:HMM:NF022428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HNF65437.1"
                     /translation="MNSEALLWGVAITAIVVANIVLVALAVIVHRRWQAAFTQAKDSA
                     SEGAALLAELRALAATQTALQAELQKSHTETLAATEALRLDVRALSAALGVFGQRLGR
                     IEEDLERRAEAAQPVGVRTELERKSIEFATRLASRGASADELVELCGLSRGEAELLMR
                     VHAKPGAKQEGAGR"
     gene            5254..5880
                     /locus_tag="PK144_06635"
     CDS             5254..5880
                     /locus_tag="PK144_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017596492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNF65438.1"
                     /translation="MTKFGGQPCWLKAPQWPLSRATGQPMRFIAQVALEAPLFAGTGG
                     RMAYLFMTDDPEQYVDGTWEPEGGENAVVVQPGHFTGPAVALAQGPSLYRMAEVAGEA
                     QLQPQAVEFRVQLRFVEEPGHQPESVRGEWTQEQQESRARALEGSKVGGTPGFLQGDE
                     FPQPGDWKLLLQLDSTTVPFHVNFGDAGIGYAFIDQAEQEGRFLWQCC"
     gene            5954..6607
                     /locus_tag="PK144_06640"
     CDS             5954..6607
                     /locus_tag="PK144_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNF65439.1"
                     /translation="MYQPASFTETRLDVLHAVMRAAPLASLVIHGPDGLDADPLPLLL
                     DAAAGPCGTLAGHCARANPLWTRAGTGGIDALAIFHGPEHYVSPAWYPAKQEHGRVVP
                     TWNYVIVQARGRLRAIDDRDWLRRQVTALTQQQEAGAAAPWAVGDAPADYLDKMLGAI
                     VGLELRIERLSGKCKLSQNQPAANRDGVIAGLQALGTSRAAVMAAEVAGRMTPPRAD"
     gene            complement(6647..7993)
                     /gene="rlmD"
                     /locus_tag="PK144_06645"
     CDS             complement(6647..7993)
                     /gene="rlmD"
                     /locus_tag="PK144_06645"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNF65440.1"
                     /translation="MARRHHRKPRAPEGVFTARIDSLAHDGRGIARVDGKVVFIEGAL
                     PGETVEYRYLATRGGYDEALTTGVLEASPERVAPRCRHFGVCGGCSLQHLAADRQIAF
                     KHDQLVENLERIGRVTPAHWLAPLTGPHWGYRQKARLGVRFVPKKDKVLVGFREKHAP
                     YLADLLGCEVLHPSVGTRIEALSALIGSLSIKTRLPQIEVAVGEDGVALNFRVMDPPS
                     AEDCEKLAAFGREHGIRILLQPGNAGSTHALWPDEIADLHYRLPAYDVELAFRTQHFT
                     QVNADINRQMIARAIELLDVQPGQHVLDLFCGLGNFTLALARVTGAEGRVSGVEGEPA
                     LVEWAGRNAAANGIGNAGFHVADLTTDITAQTWRQARYDRILLDPPRSGALEMMPHVA
                     AMGASRIVYVSCHPATLARDAAELVHTHGYRLEAAGVMDMFPHTTHVESIALFVRD"
     gene            complement(8057..8842)
                     /locus_tag="PK144_06650"
     CDS             complement(8057..8842)
                     /locus_tag="PK144_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF65441.1"
                     /translation="MNVLVFDIETIPDTDGARRLDAGLAGLDDAQVADAMFHLRRQET
                     GSDFIRHHLQRVVAISAVLRSGSQLRVWSLGTPQSGEAELIQRFFDGIGKYTPTLVSW
                     NGSGFDLPVLHYRALLHGVQAPRYWETGDSEPGFRYNHYLARFHWRHTDLMDVLAGYQ
                     PRACAPLDEIATLLGLPGKLGMDGSKVWDAYRAGRLDAIRAYCETDVLNTYLVWLRFE
                     LMRGNLGPEGYAGECRLLRDWLVQNPQPHFDEFRAGWTNCPGD"
     gene            complement(8874..9776)
                     /gene="cysM"
                     /locus_tag="PK144_06655"
     CDS             complement(8874..9776)
                     /gene="cysM"
                     /locus_tag="PK144_06655"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNF65442.1"
                     /translation="MIDPSLFPTLEDLVGETPLMRLQRLGRDLPGLVLAKLEGNNPAG
                     SVKDRPAVSMIREAEARGDIRPGDTLIEATSGNTGIALAMAAAIKGYRMILIMPDNLS
                     LERRATMKAFGAELILVTRAQGMEGARDLALSMQAEGKGRVLDQFSNPDNPLAHYKST
                     GPEIWRQTQGRITHFVSSMGTTGTIMGVSRYLKEVAPHVQVIGVQPADGAQIPGIRRW
                     PEAYLPKIYEAPRVDRILDVNQADAEAMTRQLAREEGLFCGISSGGAMVAALRIAAET
                     PDALIVSIVCDRGDRYLSTGVFPD"
     gene            9934..12354
                     /locus_tag="PK144_06660"
     CDS             9934..12354
                     /locus_tag="PK144_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF65443.1"
                     /translation="MIFSGLPARIALLAFVPVLGLAALASAWLGYERYQAGVQEINTR
                     GTTLVRQAALALEGDPVLERERLRRYALGLLALDDVLTVSIDGLDGQTLVREQRSSSQ
                     RLNGNAGEQPEASLGFEAPVRGALAASLTPDISPGPGYLPGPARVHIELSRAPLFALR
                     SSVLIDTLGLLGLGALFGLLAASLGSIAILTPFERVQRLLNRLRNGQFEPPLRRGDDG
                     GPLDALEEGISAIARLLREQRQTLMSRDEQTMAELHETLEELEIKNAELDIARKKALA
                     ASQVKSEFLANMSHEIRTPMNGVLGFVELLAKTELSRTQQGHLQTIRASARHLMTILN
                     DILDFSKIEAGKFELAQQPFDLRELLENAVLLFAPNAHAKNLSLVLDVAPELPSGLIG
                     DGPRIAQIVTNLVSNAVKFTDRGQVEVCVRLCSETAKTVTFGIAVLDTGIGFAPQDRA
                     RLFSAFDQIDTSPTRRHGGTGLGLAICRRLVQMMGGDIHAESVPGEGACFEATLRLDR
                     LLATEAGEGAEAVPPPVSMPALLAMRDETLARALTHILALRGVAASLCRDVNELEAAL
                     SQPPAPGPLLIAIDLRTLPGARQALPELLRAREQPVEGGLLLIGGAEAAMPGNGGFAY
                     GRLQAWQIARPPVSREVLPVLDALLARHAGTPLPDQASTTVPAAHSTPGTQGLHVLLA
                     DDNLINRQLARLFLQQLGAQVDEAANGLEAVQRCAGHDYDVVLMDIQMPELDGLSATR
                     RIRAAGPNRDTPIVALTAEILPSDAGRYRAAGIDDQVIKPVTEEALVATLLKWGNRCH
                     LPDPQVQA"
     gene            12488..>12690
                     /locus_tag="PK144_06665"
     CDS             12488..>12690
                     /locus_tag="PK144_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65444.1"
                     /translation="MTELPVFDPQEALRVTGNRAAIATHLLGLFLNELPEARLVITDL
                     AADGEREALRARVHKLAGSAMYCG"
BASE COUNT         1862 a         4520 c         4477 g         1831 t
ORIGIN      
        1 cgtggcgccg ctgcgtgcgc cggtcgagcc gctgctgcgc gaggcgcgcc cggccgccag
       61 ccggtcggcg ggcgttgcgg ccgctgccgc gccggcgcgt caggaatccg gcgcccgcca
      121 caacgtcacc tgggcgcagg acccggtgat cacgcagatg cagaccgaaa tctcggcgct
      181 gcgccagctg ctcgaacagc agctgacctc gatcgcctgg gggcgcgagg gctcgcgtca
      241 gccgcggcgc ttcgagctgc tcgaacgcct gctgaagttc ggcctcgacg ccgagctctg
      301 ctacgaactc tgcgatgccg cgctcagcga gcccgacaac gagcgggcct ggcagtccgc
      361 gctcggccag ctgcaccgcg gcatcgtgac gatggacgac gatccgctcg atcgcggcgg
      421 tgccatcgcg ctggtcggcc cgaccggggt cggcaagacc acgacggttg ccaagctcgc
      481 cgcgcgctac gcgctgcgcc acggcccgcg caaggtcggc ctgatcacca ccgacggcta
      541 ccgcatcggc gcctacgatc agctgcgcac ctacggcata ctgctcgacc tgccggtgcg
      601 cgtcgccggc gatgcgccgg agctgcgtca ggcgctgtcg gatttctccg acaagtcgct
      661 ggtgctgatc gacaccgccg gcatgggcct gcgcgacgtg cggctggcgc agcagatcgg
      721 catgctgcag accgcgccgg ccatccacaa ctatctggtg ctgccggcca acggtcatcc
      781 ggccgcgctc ggcgagagcg tgcaggcctt cgccggcgtg cagctgaccg gtggcatcct
      841 cagcaagctc gacgaggccg cgcgcatcgc cggcgtgctg tcgctgctgt atcgcaccgg
      901 cctgccgctc gcgtggttca cgaacggcca gcgcgtgccc gaagacatcg tcagtgcccg
      961 cgccgagacc ctgatggcga cggcactcga ccagcagctc gatgattgcg gcatcgccga
     1021 tgacgcgatg ctggtcacct ggggaaggag aatcgtaaat gcctgccagt gaagcgatgc
     1081 gtccggagca gggcagcgcg ccacgccgtg ccgcgcccca gaatccggtc cgcgtcatct
     1141 gcgtggccag cggcaagggc ggcgtcggca agaccagcac gacgatcaac ctcgccgtcg
     1201 cgctcgccac cctgcgccgc cgcgtgctga tcctcgatgc cgacctgggg cttgccaacg
     1261 tcgacgtgct gctcggcctg catccggtct ggaacctcgc gcacgtgctg gccggggagc
     1321 ggacgctgga ggagatcctg ctcgacggcc cgcacggcat ccgcatcgtg ccggcaagct
     1381 ccggcatcaa gcgcatggcc gagctgtcgc cggtcgagca cgccggcatc atcagcgcct
     1441 tcagcgagct cggcgatgcc ttcgacgtgc tgctggtcga ttgcgcggcc ggcatttccg
     1501 acagtgtcgt caccttcgcg cgcgccgcgc aggaggtcgt ggtcgtggtc tgcaacgagc
     1561 cggcctcgat caccgacgcc tacgcgctga tcaagctgct gtcgcgcgac tacggcgtcg
     1621 accacctgca cgtgctcgcc aaccgcgtcg ccggtcaccg cgagggcgtg gacctctaca
     1681 ccaagctcgt gaaggtcacc gaccgcttcc tcgacgtgac gctgtcgcac atcggcagca
     1741 tcccggagga cgcccagctg cgccgcgcca tccagatgca gcaggccgtc gtcgatgcct
     1801 ttccgagtgc gcgcgccagt gcgagcttcc tgcgccttgc cgagaacatc gagcgctggc
     1861 cgctgccggc cggcccgagc ggccatctgc agttcttcct cgaacggctg gtcggtgccg
     1921 gcgtgcgctg acgcgccgcg ccccgccgcc ccacgggaga cccgacacca tgttgcgcgc
     1981 atcctgtccc tacgcgcctg cccgacccgg actcgacgag ctggtcgccc ggcatgcgcc
     2041 gcaggtcaag cgcattgcct gtcatctgct cgcacgcctg ccggcctcgg tgcagatcga
     2101 cgacctgatc caggccggca tgctcggcct gatcgaggcg gcgcgccgct acgacgccag
     2161 ccagggcgcg agcttcgcga ccttcgccga acagcgcatc cgcggcgcga tgctcgacga
     2221 gatccgccgc ggcgactgga cgccgcgctc ggtgcaccgg cgctcgcgcg aggtcgccgc
     2281 cgcgatccgc gcgatcgaga ccgaaaccgg ccgcgaggcc caggacaccg atgtcgcgct
     2341 gcgcctcggc ctgagcctgc ccgagtacca cgacacgctg accgagctgc gcgggcagaa
     2401 gctcgtgtcg ctcgatgacg acgagcacgg cgaggacgaa ggcgagggcc tgcttgcgca
     2461 gctcgcctcc accgaggccg gtcccgagga ggaaaccgcg cgcgagcagt tgctcggcgc
     2521 cgtctccgcg cagatcgatg cgctgcccga gcgcgagaag ctcgtgctgg cgctctatta
     2581 cgacgagggc ctgaacctca aggagatcgg cgcggtgctc ggtgtcagcg agtcgcgcgt
     2641 ctgccagatc cacgcgcagg ccgtcgcgcg gctgcgctcc cgcctgcgct gagcgaccgg
     2701 ggagccccta cgcccatgga catcctcagt ctcgccggca tcgtgctcgc gctggtcgcg
     2761 atcatcggcg gccatctgat cgaaggcggc agcctggtat cgctgttcaa cccggcggcc
     2821 ttcgtgatcg tcgtgctcgg caccatcgcc gcgagcctgc tcggcacgcc gatgaccgtg
     2881 ttccgcggcg cgctgcgccg gctgcgctgg gtgttcgtgc cgccgcccgc cacgcacgag
     2941 gaactgatcg agaagatcgt cggctggagc cagatcgccc gccgtgccgg cctgctcggt
     3001 ctggaagaca tcatcgagcg cgagaccgag ccgttcacgc gcaaggccct gcagatgctg
     3061 gtcgacggcg ccgatgccga gaccatccgc cacgcgatgg agatggagct cgccgcgcgc
     3121 gaggacttcg aactgcgcgc cgcgcgcctc tacgagagca tgggcggcta ctcgccgacc
     3181 atcggcatcc tcggtgccgt gctcggtctg atccacgtga tggagaacct cgccgatccg
     3241 tcgaagctcg gcagcggcat cgccgcggcc ttcgtcgcga ccgtctacgg tgtcggcttc
     3301 gcgaacctcg tgctgctgcc gatcgccaac aagctcaaga gcgtgatcaa ccgccaggcc
     3361 cagctcgccg agatggtcat cgagggcgtg ctcgcgatcg ccaccggcga gaatccgcgc
     3421 aacatcgaga tgcggctgcg cggcttcatc cacgaatgaa gcggcgctga tccccggcgg
     3481 ctgcatccgc cctccgcctt ccgtcattcc gcccgcaccg cctgtccgga acccgcagcc
     3541 atgcgccatc accgccgtgc cgacgaccac gagaaccacg atcgctggct ggtttcctac
     3601 gccgacttca tcacgctgct gttcgcgttc ttcgtggtca tgtacgcgat cagctcggtc
     3661 aacgccggca agtaccgcgt gctgtccgag aacctctatt cggccttccg ctcgccgatc
     3721 agcggcggcg cgctgccgat cggctccgag ccgcaggcgc tctccgaccc ccaggacgga
     3781 cgcaagccca acctgatcgc cccggacctg cgcccgctgc cgacgccgcc gatgcgcccg
     3841 gaccagcggc cggtgctcga tcgcagcacc ggccccgacc gccagacgcc gccgctggcg
     3901 cgaccggtgc cggagaacgc cccgcacgcc gagcagccgc agcccggcgc gccgccggcc
     3961 gatgccagcc agcagcagct cgacgcggtc gccggcgccc tgcgcaacgc catgaccgac
     4021 atgctgcagt cccagcagat ggaggtgcgc cgcgagaagc actgggtgga gctggagatc
     4081 aaggacaacc tgctgttccc gagcggcagc gcgcagatca acaaccaggc ccaggacccg
     4141 ctcgcgcgca tcgccggcgt gctgaagaac ctgccgaacc gcgtgcaggt cgagggctat
     4201 accgacaacg tgccgatccg caacgccgtc tatccgtcga actgggaact gtcggcggcg
     4261 cgcgcggcca gtgtcgtcga agtgctcgcg cacgccggcg tcgatgcgca gcgcatggcc
     4321 gcgatcggct acggcgagca ccgcccggtc gccgacaatg ccagcgccga tggccggctg
     4381 cgcaaccggc gcgtggtcat ggtcgtgctg aaccagcccg atccgcgcgc cgatgtcgcc
     4441 gtcgacccgg acgctgccgg caccaacggc ccgcgtgcca ccgataccgc cgggactgcg
     4501 ccatgaactc cgaagccttg ctgtggggcg tcgcgatcac cgcgatcgtg gtcgccaaca
     4561 tcgtgctggt ggcgctggcc gtcatcgtcc accgccgctg gcaggcggcg ttcacgcagg
     4621 cgaaggacag cgccagcgag ggcgcggcgc tgctcgccga gctgcgcgcg ctggccgcga
     4681 cccagacggc gctgcaggcc gagctgcaga aatcccacac cgaaacgctc gcggccaccg
     4741 aggcgctgcg cctggacgtg cgcgcgctgt ccgcggcgct cggcgtgttc ggccagcggc
     4801 tcgggcgcat cgaggaagac ctcgaacgcc gcgccgaggc cgcgcagccg gtcggcgtgc
     4861 gcaccgagct cgaacgcaag tcgatcgagt tcgccacgcg cctggcctcg cgcggggcct
     4921 cggccgatga actcgtcgag ctgtgcgggc tgtcgcgcgg cgaggccgag ctgctgatgc
     4981 gcgtgcatgc gaagccgggc gccaagcagg aaggcgcggg gcgctgaagc cgccggtttc
     5041 ccgccccggt gcctgagggg ggcctgaggg cggatcgcgg tcgcacccgg ctccggcccg
     5101 atgccggttg acacgcccgc agatcgcggc ctggaatcgc ctgccgccgt gcacagctgc
     5161 ggcggcagcg ccaggaatcc ggggagaacc gccatcatgg cccgtgctgg aaccctcctt
     5221 ttcgaacccg tccgcgaagc ggtcacggcc tgcgtgacca agttcggcgg gcagccctgc
     5281 tggctgaaag ccccgcaatg gccgctgagc cgcgcgaccg gccagccgat gcgcttcatc
     5341 gcgcaggtgg cgctggaggc cccgctcttt gccgggaccg gcggccgcat ggcgtatctg
     5401 ttcatgaccg atgatccgga gcagtacgtc gatggcacct gggagccgga gggcggggag
     5461 aacgccgtcg tcgtgcagcc cgggcacttc acgggaccgg ccgtggcgct ggcgcaggga
     5521 ccgtcgctgt accggatggc cgaagtcgcc ggggaggcgc agctgcagcc gcaggccgtc
     5581 gaattccggg tgcagcttcg gttcgtggag gagcccgggc atcagccgga atcggtgcgc
     5641 ggcgaatgga cgcaggagca gcaggagtcc cgggcgcggg cgctggaagg cagcaaggtc
     5701 ggcggaaccc ccggattcct gcagggcgac gagtttccgc agcccggcga ctggaagctg
     5761 ctgctgcagc tcgattccac caccgtgcct tttcacgtga acttcggcga tgccggcatc
     5821 ggctatgcgt tcatcgacca ggccgagcag gagggccggt ttctgtggca gtgctgctga
     5881 cgggaaccgg caggatcccg ggctttccgg ctatgttcgc ccgtcgcccc ggccctgcat
     5941 cggagtccgc gccatgtacc agcccgccag cttcaccgaa acccgcctcg acgtgctgca
     6001 tgccgtgatg cgcgccgcgc cgctcgcgag cctcgtcatc cacggccccg acggcctcga
     6061 tgccgacccg ctgccgctgc tcctcgatgc cgccgccgga ccgtgcggca cgctcgccgg
     6121 ccactgcgcg cgcgccaatc cgctgtggac gcgggccggc accggcggca tcgacgcgct
     6181 cgcgatcttc cacggccccg agcactacgt gtcgccggcc tggtatccgg ccaagcagga
     6241 acacggccgc gtcgtgccga cctggaatta cgtgatcgtg caggcccgcg gccggttgcg
     6301 cgcgatcgat gaccgtgact ggctgcgccg gcaggtcacg gcgctgacgc agcagcagga
     6361 agccggtgcc gccgcgccgt gggcggtcgg cgatgcgccg gccgattacc tcgacaagat
     6421 gctcggcgcc atcgtcgggc tggagctgcg catcgagcgg ctgagcggca aatgcaagct
     6481 cagccagaat cagccggcgg cgaatcgcga cggcgtgatc gcggggctgc aggcactcgg
     6541 cacttcccgg gctgcggtga tggcggccga ggtggcgggg cgcatgacgc cgccgcgcgc
     6601 ggactgaacg accgcgggcc gcaggcgcct gcgccgtgcc ggcttttcag tcgcgcacga
     6661 acagcgcgat cgattcgacg tgcgtcgtgt gcgggaacat gtccatgacg ccggcggctt
     6721 cgagccggta gccgtgcgtg tgcacgagtt cggcggcgtc gcgcgcgagg gttgccgggt
     6781 ggcaggagac gtagacgatg cgcgaggcgc ccatcgcggc gacgtgcggc atcatctcca
     6841 gcgcaccgga gcgcggcggg tcgagcagga tgcggtcgta gcgcgcctgg cgccaggtct
     6901 gcgccgtgat gtcggtcgtc aggtcggcga catggaagcc ggcgttgccg atgccgttgg
     6961 ccgcggcgtt gcgcccggcc cattcgacca gcgccggttc gccctcgacg ccgctgaccc
     7021 gtccttcggc gccggtgacg cgcgccagcg ccagcgtgaa gttgccgagg ccgcagaaca
     7081 ggtcgagcac gtgctggccc ggctgcacgt cgagcagttc gatcgcacgc gcgatcatct
     7141 gccggttgat gtcggcgttg acctgcgtga agtgctgcgt gcgaaacgcg agttccacgt
     7201 cgtacgccgg cagccggtag tgcaggtcgg cgatctcgtc cggccacagc gcatgggtcg
     7261 agccggcatt gccgggctgc agcaggatgc ggatgccatg ctcgcggccg aaggcggcga
     7321 gcttttcgca gtcctcggca ctcggcgggt ccatgacccg gaagttcagc gccacgccgt
     7381 cctcgccgac cgcgacctcg atctgcggca ggcgggtttt gatgctgagg ctgccgatca
     7441 gcgccgacag cgcctcgatg cgcgtgccga cgctcgggtg cagcacctcg cagccgagca
     7501 ggtcggcgag atagggcgcg tgcttctcgc gaaagccgac cagcaccttg tccttcttcg
     7561 gcacgaagcg cacgccgagc cgcgccttct gacggtagcc ccagtgcggg ccggtcagcg
     7621 gcgccagcca gtgcgcgggc gtcacgcggc cgatgcgttc gaggttctcg acgagctggt
     7681 cgtgcttgaa cgcgatctgc cggtcggccg ccagatgctg caggctgcag ccgccgcaga
     7741 cgccgaagtg ccggcagcgc ggcgccacgc gctcggggct cgcttcgagc acgccggtgg
     7801 tcagcgcctc gtcgtagccg ccgcgggtgg ccagatagcg gtattcgacg gtctcgccgg
     7861 gcagcgcgcc ttcgatgaac acgaccttgc cgtcgacgcg ggcgatgccg cggccgtcgt
     7921 gggcgaggct gtcgatgcgg gcggtgaaca cgccttccgg cgcgcggggt ttgcggtggt
     7981 gacgacgggc catcggagcg gaaaccggat cagggagggg ggatacgaag gcggcgggca
     8041 ggggcggcga cagccgtcag tccccggggc agttcgtcca gccggcgcgg aactcgtcga
     8101 aatgcggctg cgggttctgg acgagccagt cgcgcagcag ccggcattcg ccggcgtagc
     8161 cctcggggcc gaggttgccg cgcatcagct cgaagcgcag ccagaccaga taggtgttca
     8221 gcacgtcggt ctcgcagtag gcgcggatcg catcgagccg gccggcgcgg taggcgtccc
     8281 agaccttgct gccgtccatg ccgagcttgc cgggcaggcc gagcagcgtc gcgatctcgt
     8341 cgagcggcgc gcaggcgcgc ggctggtagc cggcgagcac gtccatcagg tcggtgtgcc
     8401 gccagtggaa gcgcgcgagg tagtggttgt agcggaagcc cggttcggaa tcgccggtct
     8461 cccagtagcg cggcgcctgc acgccgtgca gcagcgcgcg gtagtgcagc accggcaggt
     8521 cgaaaccgct gccgttccag ctcaccagcg tcggcgtgta cttgccgatg ccgtcgaaga
     8581 agcgctggat cagctcggcc tcgccggatt gcggcgtgcc gagcgaccag acgcgcagct
     8641 ggctgcccga gcgcagcacg gccgagatcg cgacgacgcg ctgcaggtgg tggcggatga
     8701 agtccgagcc ggtttcctgg cggcgcagat ggaacatcgc atcggcgacc tgtgcgtcat
     8761 cgaggccggc gagtccggca tcgagccggc gggcgccgtc ggtgtcggga atggtctcga
     8821 tgtcgaagac gaggacgttc acgggcgctt cactgtaagt actgtaagtc cggtcagtcc
     8881 gggaacacgc cggtcgacag ataccggtcg ccgcggtcgc agacgatgct gacgatcagc
     8941 gcgtccgggg tttcggcggc gatgcgcagc gccgcgacca tcgcgccgcc cgaggaaatc
     9001 ccgcagaaca ggccttcctc gcgcgcaagc tgccgggtca tcgcctcggc atcggcctga
     9061 ttcacgtcga ggatgcgatc gacgcgcggg gcctcgtaga tcttcggcag ataggcctcg
     9121 ggccagcggc ggatgccggg aatctgcgca ccgtcggccg gctgcacgcc gatgacctgc
     9181 acgtgcggcg cgacctcctt gaggtaacgc gacacgccca tgatcgtgcc ggtcgtgccc
     9241 atggagctga cgaagtgcgt gatgcggccc tgcgtctggc gccagatttc cgggccggtg
     9301 gacttgtagt gcgcgagcgg gttgtcgggg ttcgagaact ggtcgagcac gcggcccttg
     9361 ccctcggcct gcatcgagag cgccaggtcg cgtgcgcctt ccatgccctg ggctctggtg
     9421 acgaggatca gctcggcgcc gaaagccttc atcgtcgccc ggcgctccag cgacaggttg
     9481 tccggcatga tcaggatcat ccggtagccc ttgatcgccg ccgccatcgc cagcgcgatg
     9541 ccggtgttgc cgctcgtggc ttcgatcagc gtgtcgccgg ggcggatgtc gccgcgggcc
     9601 tcggcctcgc ggatcatcga caccgccggg cggtccttga ccgagccggc cgggttgttg
     9661 ccttcgagct tggcgagcac gaggccgggc aggtcgcggc cgaggcgctg cagccgcatc
     9721 agcggggttt cgccgaccag gtcttcgagg gtcggaaaca gggacgggtc gatcatgggc
     9781 ggcaggtccg gatggggcag gggcgccgca ttcaggcggg ccggctagta tacacggccc
     9841 gttccggccg cccggccgtt gcccctgccg tccgatgctc ctcctgccgc tgcctgctgc
     9901 tgtcctgatc cacgcctgcc ggcgtgccga gcgatgatct tctccggtct gccggcgcgc
     9961 atcgcgctgc ttgccttcgt gccggtgctg gggctggccg cgctcgcttc ggcctggctc
    10021 ggctacgagc gctatcaggc cggcgtgcag gagatcaaca cccgcgggac gacgctggtg
    10081 cgccaggcgg ccctggccct ggagggcgat ccggtgctcg aacgcgagcg cctgcgccgc
    10141 tacgcgctcg ggctgctcgc cctcgatgac gtgctgaccg tgagcatcga cggcctcgac
    10201 ggccagacgc tggtgcgcga acagcgcagc agcagccagc gcctcaacgg caacgccggt
    10261 gagcagcccg aagcctcgct cggcttcgag gcaccggtgc gcggggcgct ggccgccagc
    10321 ctgacgccgg acatcagccc cgggccgggt tacctgccgg ggccggcgcg cgtgcacatc
    10381 gagctgtcgc gcgcgccgct gttcgcgctg cgcagcagcg tgctgatcga cacgctcggc
    10441 ctgctcgggc tcggggcgct gttcgggctg ctggccgcga gcctcggttc gatcgcgatc
    10501 ctgacgccgt tcgaacgcgt gcagcgcctg ctgaaccggc tgcgcaacgg ccagttcgag
    10561 ccgccgctgc gccgcggcga cgacggcggg ccgctcgatg cgctggagga aggcatcagt
    10621 gccatcgccc ggctgctgcg cgagcagcgc cagacgctga tgtcgcgcga cgagcagacc
    10681 atggccgaac tgcacgagac gctggaggaa ctcgagatca agaacgccga gctcgacatc
    10741 gcgcgcaaga aggcactggc cgcgagccag gtcaaatccg aattcctcgc caacatgagc
    10801 cacgagatcc gcacgccgat gaacggcgtg ctcggcttcg tcgagctgct ggccaagacc
    10861 gagctgtccc gcacccagca ggggcatctg cagacgatcc gtgcctcggc acggcatctg
    10921 atgaccatcc tcaacgacat cctcgacttc tcgaagatcg aggccggcaa gttcgaactc
    10981 gcgcagcagc ccttcgacct gcgcgagctg ctggagaacg ccgtgctgct gttcgccccg
    11041 aacgcgcacg ccaagaacct gtcgctggtg ctcgacgtgg cgcccgagct gccttcgggg
    11101 ctgatcggcg acgggccgcg catcgcgcag atcgtcacca acctcgtcag caacgccgtc
    11161 aagttcaccg atcgcggcca ggtcgaggtc tgcgtgcggc tgtgctccga aaccgcgaag
    11221 accgtgacgt tcggcattgc cgtgctcgat accggcatcg gcttcgcgcc gcaggaccgc
    11281 gcacgcctgt tcagcgcctt cgaccagatc gacacctcgc cgacgcgccg ccacggcggc
    11341 accggtctcg gactcgcgat ctgccggcgc ctggtgcaga tgatgggcgg cgacatccac
    11401 gccgagagcg tgcccggtga gggcgcgtgc ttcgaggcga cgctgcggct cgatcggctg
    11461 ctcgccaccg aagccggcga gggcgccgag gcagtgccgc cgccggtgtc gatgccggcg
    11521 ctgctggcga tgcgcgacga gacgctggcg cgcgcgctca cgcacatcct ggcgctgcgc
    11581 ggcgtggcgg cgagcctctg ccgtgacgtg aacgagctgg aggcggcgct gtcgcagccg
    11641 ccggcgccgg gaccgctgct gatcgcgatc gacctgcgca cattgccggg cgcccgtcag
    11701 gccctgcccg agctgctgcg cgcgcgcgag cagccggtcg agggcgggct gctgctgatc
    11761 ggcggggccg aggccgccat gcccggcaac ggcggcttcg cctacggccg gctgcaggcc
    11821 tggcagatcg cccgcccgcc ggtgtcgcgc gaggtgctgc cggtgctcga tgcgctgctc
    11881 gcccgccacg ccggtacgcc actgccggat caggcttcga cgacggtgcc ggcggcgcac
    11941 agcacgcccg gcacccaggg gctgcacgtg ctgctcgccg atgacaacct gatcaaccgc
    12001 cagctcgccc ggctgttcct gcagcagctc ggtgcgcagg tcgacgaggc ggccaacggg
    12061 ctggaggccg tgcagcgctg cgccggacac gactacgacg tggtgctgat ggacatccag
    12121 atgcccgaac tcgatggcct gtcggcgacg cggcgcatcc gcgcggccgg cccgaaccgc
    12181 gatacgccga tcgtcgcgct gaccgccgag atcctgccga gcgacgccgg gcgttatcgt
    12241 gccgccggca tcgacgatca ggtcatcaag ccggtcaccg aggaagccct ggtcgcgacg
    12301 ctgctgaaat ggggcaaccg ctgccacctg cccgatccgc aggtgcaggc ctgaagtgca
    12361 ggcgcgaccc gacgggccgc cggacggctt ctgaaccccc accactgcac ccgcagccgc
    12421 cggaggcgcg ccgcggagca gagaacaccg gagccatgcc atggaatgcg acaaccaagg
    12481 agcgcacgtg actgaacttc ccgtctttga cccgcaggaa gccctgcgcg tgaccggcaa
    12541 ccgtgccgcg atcgccacgc atctgctcgg cctgttcctg aacgaactgc ccgaggccag
    12601 gctcgtgatc accgatctgg ccgccgacgg cgagcgcgag gcgctgcgcg cgcgcgtgca
    12661 caagctcgcc ggttcggcga tgtactgcgg
//
LOCUS       DAOQIE010000077        12567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2448_length_12567_cov_6.552430, whole genome shotgun sequence.
ACCESSION   DAOQIE010000077 DAOQIE010000000
VERSION     DAOQIE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2448_length_12567_cov_6.552430"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PK144_06670"
     CDS             <1..1475
                     /locus_tag="PK144_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65445.1"
                     /translation="MTQLPLRSQFKFATVVLLATLALLMTTMYLHTRAGLYDQFEDRL
                     HERVELTAGILEHYHADEQAGRMDRAAAQAQAATAINSLMHEDAYFFAYDRDGIYVIQ
                     GANPAQIGKTFKGQIDPLGRDMYVEFQKAVAGSVKGIEHAGFVEYDWKRPGRETPEHK
                     VSYAKLFAPWGWVIGNGHYTADVDAQLANERWLMIGGFIVVALLMFGMMNWLSRGILQ
                     RIHTVLGFAQRVARRDLDAHMQITVHDEVGQMAEALNAAVATLRSAFAEIEAAAERDR
                     QQMLRLQAAENTAARAERLALAVREVDESLQTVAGSTSELETAVHEISRNLHEAVSVV
                     GTATRAVDAAAATVAQLTDSSARIGQVSRVINDIAEQTNLLALNATIEAARAGEAGKG
                     FAVVASEVKDLARDTARATDDIGQRIGSIQADTGQVVDAIEQIRQTIAEVNRIATMIA
                     SAVEEQSVTTREISRSVGRAAEGSAGITDEVKLMISTARG"
     gene            complement(1501..2481)
                     /locus_tag="PK144_06675"
     CDS             complement(1501..2481)
                     /locus_tag="PK144_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HNF65446.1"
                     /translation="MSTPHAIRIHEHGGPEVLRWEPFDPGAPGPGELRVRIGAVGLNF
                     IDTYHRSGLYPLALPSGLGLEGAGTVEAIGEGVSEFAPGDRVAWGTGPVGSYAEVRVM
                     PADKAVKLPDAIDERTAAAMMLQGMTACYLLRRTYRVQAGDTVLVHAAAGGVGLIACQ
                     WAASLGATVIGTVGSKEKAGLAAAHGCAHPILYREEDFVARVKEITGGAGVAAVYDGV
                     GRDTFLGSLDCLQPLGTMVTFGNSSGPVPAIEPLLLAQKGSLFLTRPTLFHYTSKRAD
                     LLATAGELFDVVASGRVRIEVAQTFPLAEAASAHRALESRATTGSTVLLP"
     gene            complement(2575..3261)
                     /gene="tsaA"
                     /locus_tag="PK144_06680"
     CDS             complement(2575..3261)
                     /gene="tsaA"
                     /locus_tag="PK144_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNF65447.1"
                     /translation="MSTTLTPVGTLHSPYREKFAIPRQPGLARLPAVLELLAPFDRPE
                     AVRGLDGFSHVWLIFGFHATAAAGWKPTVRPPRLGGNARLGVFATRSTFRPNPLGLSV
                     VELAGIRCAGGVFLDLLGADLLDGTPVYDVKPYLPYADAIPGARAGFAPEPPARLAVA
                     FAPAAAQVLAAHPEPRLREWLVAVLALDPRPAYRTGEDGDFGVRLFEFDVRAEIRGGQ
                     LTVRELRRAG"
     gene            complement(3258..5273)
                     /locus_tag="PK144_06685"
     CDS             complement(3258..5273)
                     /locus_tag="PK144_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65448.1"
                     /translation="MSLATLPLLPAAPRGLRYGLLALLLTGIALANFHLSGRAPASVL
                     LDPSWCQVLDAAATRDWVFGRDLMFTFGPFGYLHCDDGLGQHAGLRYAAALGLAFGFA
                     YLLVRGALEAGPRLGWPLLLWGALLLDVEARAYLVIAFGAVVLLWRRDGPWAALLVTA
                     LLALLSLVKFSFFVPAAFAVAVITLTLASGRQLRAAALVAGSYLGLLLALWLAAGQPL
                     GALPGYLAGSLDMSGGYAGAMSYLPRMIQPNPLLLLLVLAIWGALVGLLGLALCGTQA
                     RERHALLLIDACLIYVAWKRGFVRADALHLIGFFAILPVLGVLPMLGAMLWRASLQRW
                     GEACCGLLALIALAGIFAIDRYHPVRQIDDMRAGMRALLAGPQPGPVPAGDPKTRGTP
                     GPADRLLLTQRLAGRGSVDLISYEQGRLFFNDLNYRPRPVIQSYAAYTPALAARNAAH
                     FLGPQRPDFVLFNPAETIDGRLPMMDDGAALLVVLGNYRALGRDGKYLLLQRRDDAPR
                     PPQLESLAERELHWGEDFDLTAFRGEVLAFALEVTPSAAGRLAGLLFQSLPPQIVLDA
                     GDGERRYRLVPGMLATPVLLSPHLRTSEDLLALGAPPPAAGFGHAVRRLRFEVAPPAP
                     LLAGHEDFGRALYHDTLKLRLYRVRGLPDAGFTAAYSPDPDRVRAGP"
     gene            complement(5290..5952)
                     /locus_tag="PK144_06690"
     CDS             complement(5290..5952)
                     /locus_tag="PK144_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF65449.1"
                     /translation="MDKGTALRDEYRARFAGMDEYRLGVWRIICGEYLSRWITADAAV
                     LDLGCGWGEFSNSVTAARRYAMDLNPDAEARLPAGTRFLHQDCSQPWALADGELDVVF
                     TSNFLEHLPDKTGIERTLAEAWRCLKPGGRLICLGPNIKHVPGEYWDFWDHHIPLTEA
                     SLAEVMMLRGFHIEQRIERFLPYSMSTGRTPPLAFVRWYLRLPFAWRFFGKQFLVVGR
                     KP"
     gene            complement(6031..6453)
                     /locus_tag="PK144_06695"
     CDS             complement(6031..6453)
                     /locus_tag="PK144_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNF65450.1"
                     /translation="MKRLARVRDNPFLRFLVVGGFAAAANFGSRFVFSRGVSYETAVT
                     LAWCVGITTAFTLSRLYVFEGARHGSVWRQFAGFTLVNLFALLQTLAVSLGLLHWGLP
                     ALGIERHAEAIAHAFGIVVPIFTSYFGHKHLSFRQPAL"
     gene            complement(6450..7742)
                     /locus_tag="PK144_06700"
     CDS             complement(6450..7742)
                     /locus_tag="PK144_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF65451.1"
                     /translation="MAHVIVLGAGAMGLAAAHHLLKAGHTVELFEADRVAGGMAAHFD
                     FGGLSIERFYHFVCKADAPTFALLEELGLAQAMRWRPTKMGYFYDGKLYRWGDPLALL
                     AFPKLDPVSKLRYAWQMFMATKRTDWRDLDGISAAEWLRRGSGERAWKVLWEKLFTLK
                     FFELSEQVSAAWLWTRIKRVGTSRRSLMQEELGYIDGGSETLVKALVASIERQGGRLH
                     LGTPVREVTLEGGAVTGIVTAGGERVAAGHVISTVPMPLIPKLIPALPEALKARYAAL
                     RNIGAVCVIHKLRRAVTGNFWLNTNDARIEVPGLVEFSNLRPFADGTHIVYVPYYMPQ
                     THPKFARDDDFFVRESFGYLKLVNPQLTDADRLDSAVGRLQYAQPLCPPGFAAMLPDI
                     VTPIRGLQIADTSSYYPEDRGIAESARVAQEMAARVGA"
     gene            complement(7788..8729)
                     /locus_tag="PK144_06705"
     CDS             complement(7788..8729)
                     /locus_tag="PK144_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF65452.1"
                     /translation="MDKNDKIVVLGAAGLVGQNLIVELKAQGYRQLVAIDKHAHNLGV
                     LAQLHPDVTTVLADLAEPGPWREHLRGAARVLQLQAQITGKEAQLFVRNNLDSTKLVL
                     EAVREHQVPFLVHISSSVVNSVADDDYTNTKKAQEKLVLDSGLPCCVLRPTLMFGWFD
                     PKHLGWLSRFMAKVPVFPIPGHGRYMRQPLYNRDFCRIIVQCMERAPDRAIYDIVGDT
                     RIDYVDIIHTIRRVKGLRTPIVHIPYTLFRWLLLGYALFSDKPPFTADQLKALTAGDD
                     FTGVDTEAEFGIRQMPFEDAIRESYCDPRYAHIVLER"
     gene            complement(8774..9511)
                     /locus_tag="PK144_06710"
     CDS             complement(8774..9511)
                     /locus_tag="PK144_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF65453.1"
                     /translation="MRKVLIVGATSAIAEACARRFAAEGDALCLCARRAGRLEALAAD
                     LRVRGAALVAVEVLDANDVAGHAAMLDRATAALGGLDTVLIAHGTLSDQAACEASVEQ
                     TLAEIHTNGLSTVALLTELAGRFERQRHGTLAVISSVAGDRGRQSNYVYGSAKALVTA
                     FTGGLRQRLAKAGVNVLTIKPGFVDTPMTAAFPKGPLWASADTIAAGIVRAIERRSSV
                     VYLPGFWRPIMAIIRAIPEPVFRRIRL"
     gene            complement(9561..10868)
                     /locus_tag="PK144_06715"
     CDS             complement(9561..10868)
                     /locus_tag="PK144_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF65454.1"
                     /translation="MTATRACAASWGRYPRVVQHARPLTDRDAPLPASDTSWLPYGNG
                     RSYGDSCLNDGGGVLLARGLDRFIGFDPQSGVLACEAGVLLADILALAVPQGWFLPVT
                     PGTRYVTVGGALANDVHGKNHHRAGSFGRHLRGFELLRSDGSRRWCSAHEHADWYGAS
                     IGGLGLTGLVTRVELQLRRIANPWIAGETIRFGSLAEFDALSAESDDDYEYTVAWIDC
                     AAAAGRGLFMRGNHAPALPRTRPAAKPGRLRVPFVPPVSLINGPTLHAFNALYYHRPV
                     GGRGCTQITHYEPFFYPLDGIGDWNRIYGPHGFLQWQCALPPAAGLDALHAILAQIAA
                     SGSGSFLAVLKRFGELASPGWLSFPRPGYTLALDFPNRGAATFALLDRLDAVVREAAG
                     AIYPAKDARMSGALFRAAYPRWQRFADYLDPQFSSSFWRRVMA"
     gene            complement(10865..12259)
                     /locus_tag="PK144_06720"
     CDS             complement(10865..12259)
                     /locus_tag="PK144_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008576933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF65455.1"
                     /translation="MDLDGTLIRSDLLVESFFLLLKHRPLCLFLVPLWLWRGKAVLKY
                     EIARRVQPDYALLPYDTAFLDWLRAEHARGRPLWLCTASHRLLAEPLAEHLGLFERVL
                     ASEDGLNLSGARKRERLVAALGERGYDYAGNAAIDLEVWRHARHAISVDLPARLRPEL
                     ARVATIEREFAPRPAGLKVYAQALRLHQWVKNLLIFLPLLLSHQIDVRAIAASLTAFL
                     AFGLCASSVYLLNDLLDLPSDRRHPRKRQRPFASGALGLVRGALLIPALLALAGLLCL
                     GLPAEFFGMLTLYYALTVAYSVKLKEFVLIDVIALAGLYTSRILAGAAAVSLHNSFWL
                     LAFSVFLFLSLALVKRYAELREMLRRGRERASGRDYSTHDLALLQTLGIASAYNSVLV
                     LALYINSDTSRQLYARPELIWLLCPVLLYWVSRVWLKTHRDEMHDDPVVFAVRDRASR
                     WLLFATGLIVLAAV"
     gene            complement(12277..>12567)
                     /locus_tag="PK144_06725"
     CDS             complement(12277..>12567)
                     /locus_tag="PK144_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65456.1"
                     /translation="CRYSAGLLAMICGRSPEVDAGRAPVAITDDGHHALQQMSRSVDC
                     RSWRRAATLRSALIVAGASLPGDPPPFPSAPATGACRSRPVRLRLHRWSRSP"
BASE COUNT         2084 a         4376 c         4468 g         1639 t
ORIGIN      
        1 ccatgaccca gctgcccctg cgctcgcagt tcaaattcgc caccgtcgtg ctgcttgcaa
       61 cactggcgct gctgatgacc acgatgtacc tgcacacccg tgccggcctg tatgaccagt
      121 tcgaagatcg cctgcacgag cgggtcgaac tcacggccgg catcctggag cactaccacg
      181 cagatgagca ggcggggcgc atggatcggg ccgctgccca ggcacaggca gccaccgcga
      241 tcaattcgct catgcacgag gatgcttact tcttcgccta cgatcgcgac ggcatctacg
      301 tgatccaggg cgcaaacccg gcgcagatag gcaagacctt caaaggccag atcgaccctc
      361 tgggccgcga catgtacgtc gaattccaga aggccgtggc cggctcagtc aagggtatcg
      421 agcacgccgg cttcgtcgaa tacgactgga aacgtccggg ccgggagacg ccggagcaca
      481 aggtcagtta cgcgaagctg ttcgctccct ggggctgggt gatcggcaac ggtcattaca
      541 cggccgatgt cgacgcgcag ctcgccaacg aacgctggct gatgatcggc ggcttcatcg
      601 tcgtcgcact gctgatgttc ggcatgatga actggctgag ccgcggcatc ctgcagcgca
      661 tccataccgt gctcggcttc gcgcagcgcg tggcgcggcg cgacctcgac gcgcacatgc
      721 agatcaccgt acacgacgag gtcggccaga tggccgaggc cctgaatgct gcggtcgcaa
      781 cgctgcgctc ggccttcgcc gagatcgagg ccgccgccga gcgcgatcgc cagcagatgc
      841 tgcgcctgca ggcggccgag aacaccgcgg cgcgtgccga gcggctggcg ctcgccgtgc
      901 gcgaggtcga cgagagcctg cagaccgtcg ccggctcgac ctccgaactg gagaccgcgg
      961 tgcacgagat ttcccgcaac ctgcacgagg cggtcagcgt cgtcggcacc gcgacgcgcg
     1021 ccgtcgacgc cgcggcggcg acggtcgcgc agctcaccga cagcagcgcg cgcatcggtc
     1081 aggtcagccg cgtcatcaac gacatcgccg agcagaccaa cctgctggcg ctgaacgcga
     1141 ccatcgaggc cgcccgcgcc ggtgaggccg gcaagggctt cgcggtggtc gcgagcgagg
     1201 tcaaggacct cgcccgtgac accgcgcgcg cgaccgacga catcggccag cgcatcggct
     1261 cgatccaggc cgacaccggt caggtcgtcg acgcgatcga gcagatccgc cagaccatcg
     1321 ccgaggtcaa ccgcatcgcg accatgatcg cgtcggcggt cgaggagcag agcgtcacga
     1381 cccgcgagat cagccgcagc gtcgggcgcg ccgccgaagg cagcgccggc atcaccgacg
     1441 aggtcaagct gatgatcagc accgcgcgcg gctgaggccg cgccggcgcc ggtcaccgtt
     1501 tcagggcagc agcaccgtcg agccggtggt cgcgcgcgac tccagcgcgc ggtgcgcgct
     1561 ggcggcctcg gcgagcggga aggtctgcgc gacctcgatg cgcacccggc cgctcgcgac
     1621 cacgtcgaac agctcgccgg ccgtcgccag caggtcggcc cgcttcgacg tgtagtggaa
     1681 cagcgtcggc cgggtcagga acagcgaacc cttctgcgcg agcagcagcg gctcgatcgc
     1741 cggcaccggc ccggaggaat tgccgaaggt caccatcgtg ccgagcggct gcaggcagtc
     1801 gagcgagccg aggaaggtgt ccctgccgac gccgtcgtag acggccgcga ctccggcccc
     1861 gccggtgatc tccttcacgc gcgcgacgaa atcctcctcg cggtacagga tcgggtgcgc
     1921 gcagccgtgc gcagcggcca gcccggcctt ctccttcgag ccgaccgtgc cgatcacggt
     1981 cgcgccgagc gaggcagccc actggcaggc gatcagcccg acgccgccgg ccgccgcgtg
     2041 cacgagcacc gtgtcgccgg cctgcacgcg atacgtgcgc cgcagcaggt aacacgccgt
     2101 catgccctgc agcatcatcg ccgcggccgt gcgctcgtcg atcgcatcgg gcagcttcac
     2161 ggccttgtcg gccggcatca cgcgcacctc ggcgtagctg ccgaccggcc cggtgcccca
     2221 ggcgacgcga tccccgggcg cgaactcgct gacgccctcg ccgatcgcct cgaccgtgcc
     2281 ggcgccttcg agcccgagtc cgctcggcag cgccagcgga tacaggccgc tgcggtgata
     2341 ggtgtcgatg aagttcaggc cgacggcgcc gatgcgcacg cgcagctcgc cgggccccgg
     2401 cgcgccgggg tcgaacggct cccagcgcag gacttcggga ccgccgtgtt cgtggatgcg
     2461 gatcgcgtgc ggcgtgctca tgggcggatg gcctcggctg acgggggaac ggacggcgca
     2521 cagtgtggcc gctcgcaccg catctgccgc ccgctgcatg gcagccgtgc aggatcagcc
     2581 ggcccggcgc agctcgcgga cggtcagctg tccgccgcgg atctccgcgc gcacatcgaa
     2641 ctcgaacagg cgcacgccga agtcgccgtc ctcgccggtg cggtaggccg ggcgcgggtc
     2701 gagtgcgagc accgcgacca gccattcgcg caggcgcggc tcggggtgcg ccgccagcac
     2761 ctgcgcggca gccggcgcga acgccaccgc gaggcgcgcc ggcggctcgg gcgcgaagcc
     2821 ggcgcgggcc ccggggatgg catcggcgta cggcagatag ggcttcacgt cgtagaccgg
     2881 cgtgccgtcg agcaggtcgg caccgagcag gtcgaggaac acgccgcccg cgcagcggat
     2941 gccggcgagt tcgacgaccg ataatccgag cggattcggc cggaaggtcg agcgcgtcgc
     3001 gaacacgccg agccgggcat tgccgccgag gcgcggcgga cgcacggtcg gcttccagcc
     3061 ggcggcggcg gtggcgtgga agccgaagat cagccagacg tgcgagaagc cgtcgagccc
     3121 gcgcacggcc tccggccggt cgaacggcgc cagcagttcg agcacggccg gcagccgcgc
     3181 cagccccggc tggcgcggga tcgcgaactt ctcgcgatac ggcgagtgca gcgtgccgac
     3241 cggggtcagc gtcgtgctca cggtccggcg cggacgcggt cggggtcggg gctgtaggcg
     3301 gccgtgaacc cggcgtccgg cagcccgcgc acgcggtaca ggcgcagctt cagcgtgtcg
     3361 tggtacagcg cccggccgaa gtcctcgtgg ccggcgagca gcggcgccgg cggcgcgacc
     3421 tcgaagcgca gccggcgcac ggcgtgaccg aagcccgcgg ccggtggtgg tgcaccgagc
     3481 gcaagcagat cctcgctcgt gcgcaggtgc ggactgagca gcaccggcgt ggcgagcatg
     3541 ccgggcacca gccggtagcg gcgctcgccg tcgccggcat cgagcacgat ctgtggcggc
     3601 aggctctgga acagcagccc ggccagccgc ccggccgcgc ttggcgtgac ctccagcgcg
     3661 aaggccagca cctcgccacg gaaggccgtc agatcgaaat cctcgcccca gtgcagctcg
     3721 cgctcggcca gcgactccag ctgcggcggc cggggcgcat cgtcgcgccg ctgcagcagc
     3781 aggtacttgc cgtcgcgacc gagcgcccgg tagttgccga gcaccacgag cagggccgcg
     3841 ccgtcatcca tcatcggcag ccggccgtcg atggtctcgg ccgggttgaa cagcacgaaa
     3901 tccgggcgct gcgggccgag gaaatgcgcg gcgttgcgcg cggcgagcgc tggcgtgtag
     3961 gccgcgtaac tctggatgac cggccgcggc cgatagttga gatcgttgaa gaacagcctc
     4021 ccctgttcat aactgatcag gtcgacgctg cccctgccgg cgaggcgctg cgtcagcagc
     4081 agccggtcag ccggacccgg tgtgccgcgt gtcttcggat cgccggccgg caccggcccg
     4141 ggctgcggcc cggcgagcag cgcccgcatg cccgcgcgca tgtcgtcgat ctggcggacc
     4201 gggtgatagc gatcgatggc gaatatgccg gccagcgcga tcagcgcgag caatccgcag
     4261 catgcctcgc cccagcgctg cagcgacgcc cgccatagca tggcgccgag catcggcagg
     4321 acgccgagca cgggcaggat tgcgaagaaa ccgatcaggt gcagggcgtc ggcgcgcacg
     4381 aagccgcgct tccaggccac gtagatcagg caggcatcga tcagcagcag cgcatggcgt
     4441 tcccgtgcct gcgttccgca cagcgccaac cccagaagcc ccaccagtgc cccccagatc
     4501 gccaggacca gcagcaggag cagcggattc ggctggatca tgcgcggcag atagctcatc
     4561 gcaccggcgt accctccgga catgtcgagg ctgccggcca gatagccggg cagcgcaccg
     4621 agcggctgac cggcagcgag ccacagggcg agcagcaggc cgaggtagct gcccgccacc
     4681 agcgccgcag cccgcagctg gcggccgctc gcgagcgtca gcgtgatcac ggccacggca
     4741 aaggccgccg gcacgaagaa cgagaacttg accagcgaca gcagcgcgag cagcgccgtc
     4801 accagcagcg ccgcccacgg accgtcgcgg cgccacagca gcacgaccgc accgaaggcg
     4861 atgaccagat aggcgcgcgc ctcgacatcg agcagcagcg cgccccagag gagcagcggc
     4921 cagcccaggc gcggaccggc ctcgagcgcg ccgcgcacga gcagatacgc aaaaccgaag
     4981 gccagcccca gcgccgcggc atagcgcagc ccggcgtgct ggccgagacc gtcatcgcag
     5041 tgcaggtagc cgaacggccc gaacgtgaac atcaggtcgc gaccgaacac ccagtcccgc
     5101 gtcgccgcgg catcgagcac ctgacaccac gacggatcga gcagcaccga ggccggtgcc
     5161 cgcccgctca ggtggaagtt ggccagcgcg atgcccgtca ggagcagcgc gagcagcccg
     5221 tagcgcaggc cgcgcggcgc ggcgggcagc agcggcaggg tcgcgagcga catggtgcct
     5281 ccctgcctct cagggcttgc ggccgacgac gaggaactgc ttgccgaaga agcgccacgc
     5341 gaacggcagg cgcaggtacc agcgcacgaa ggccagcggc ggcgtgcgcc cggtcgacat
     5401 cgagtacggc aggaagcgct cgatgcgctg ctcgatgtgg aagccgcgca gcatcatgac
     5461 ctcggcgagc gaggcctcgg tcagcgggat gtggtggtcc cagaagtccc agtactcgcc
     5521 cggcacatgc ttgatgttcg gcccgaggca gatcagccgt ccgcccggct tcaggcagcg
     5581 ccaggcctcg gcgagcgtgc gctcgatgcc ggtcttgtcc ggcaggtgtt cgaggaagtt
     5641 gctcgtgaac accacgtcga gctcgccatc ggcgagagcc cagggctgcg agcagtcctg
     5701 atgcaggaag cgcgtgccgg ccggcagccg cgcctcggcg tccgggttca ggtccatcgc
     5761 gtagcggcgg gcggccgtga cgctgttgct gaactcgccc cagccgcagc cgaggtcgag
     5821 cacggccgca tcggccgtga tccagcggct caggtactcg ccgcagatga tccgccagac
     5881 cccgagccgg tactcgtcca tcccggcgaa acgcgcacgg tattcgtcgc gcagcgctgt
     5941 gcccttgtcc acgtctgcca ctgccagtcc cccctcggga aaatcaggaa aaaacgtacg
     6001 gacgcccccg atcgcacccg gccacgcgcc tcagagcgcc ggctggcgga acgacaggtg
     6061 cttgtggccg aagtagctcg tgaagatcgg cacgacgatg ccgaaggcat gcgcgatcgc
     6121 ctcggcgtgg cgctcgatgc cgagcgccgg cagcccccag tgcagcagcc cgaggctgac
     6181 cgccagcgtc tgcagcagcg cgaacaggtt cacgagcgtg aagccggcga actggcgcca
     6241 gacggaaccg tggcgcgcgc cctcgaacac gtacagccgc gacagcgtga acgcggtcgt
     6301 gatgccgacg caccaggcca gcgtcacggc ggtttcgtag ctcaccccgc ggctgaacac
     6361 gaagcgcgag ccgaagttgg ccgctgccgc gaagccgccg acgaccagga aacgcaggaa
     6421 ggggttgtcc cgcacgcggg cgagccgctt cacgccccga ccctcgccgc catttcctgc
     6481 gcgacccgcg cgctctcggc gatgccgcgg tcctcggggt agtacgagga ggtgtcggcg
     6541 atctgcaggc cgcggatcgg cgtgacgatg tcgggcagca tcgccgcaaa gcccggcggg
     6601 cagagcggct gcgcgtactg caggcgcccg accgccgaat cgaggcggtc ggcgtcggtc
     6661 agttgcggat tgacgagctt caggtagccg aaggattcgc gcacgaagaa gtcgtcgtcg
     6721 cgcgcgaact tcggatgcgt ctgcggcatg tagtacggca cgtagacgat gtgcgtgccg
     6781 tcggcgaagg gccgcaggtt cgagaactcg accagacccg gcacctcgat gcgcgcatcg
     6841 ttggtgttga gccagaagtt gccggtgacc gctcggcgca gcttgtggat cacgcacacg
     6901 gcgccgatgt tgcgaagcgc cgcgtagcgg gccttgagcg cttcgggcag cgccgggatc
     6961 agcttcggga tcagcggcat cggcacggtg ctgatgacgt gaccggccgc gacgcgctcg
     7021 ccgccggccg tgacgatgcc ggtcaccgca ccgccttcga gcgtgacctc gcgcaccggc
     7081 gtgccgaggt gcaggcgccc gccctggcgc tcgatgctcg cgaccagcgc cttcaccagc
     7141 gtctcggagc cgccgtcgat gtagccgagt tcctcctgca tcaggctcct gcgcgaggtg
     7201 ccgacgcgct tgatgcgcgt ccacagccac gcggccgaga cctgctccga cagctcgaag
     7261 aacttcagcg tgaagagctt ctcccagagc accttccagg cgcgctcgcc gctgccgcga
     7321 cgcagccact cggcggcgct gatgccgtcg agatcgcgcc agtccgtgcg cttcgtggcc
     7381 atgaacatct gccacgcgta gcgcagcttc gagaccgggt cgagcttcgg gaaggcgagc
     7441 agcgccagcg ggtcgcccca gcggtacagc ttgccgtcgt agaaatagcc catcttcgtc
     7501 ggccgccagc gcatggcctg agcgaggccg agttcttcga gcagcgcaaa ggtcggtgcg
     7561 tcggccttgc agacgaagtg atagaagcgc tcgatgctca ggccgccgaa gtcgaaatgc
     7621 gcggccatgc cgccggcgac ccggtcggcc tcgaagagct cgaccgtgtg accggccttc
     7681 agcaggtgat gcgcggcggc gaggcccatg gcgccggcgc cgagcacgat gacgtgtgcc
     7741 atgagggctg gattctccgg gtagggggcg agtgcaggcg cgagcgctca gcgttcgagc
     7801 acgatgtgcg cgtagcgcgg atcgcagtag ctttcgcgga tcgcgtcctc gaacggcatc
     7861 tgccggatgc cgaattcggc ttcggtgtcg acgccggtga aatcgtcgcc ggcggtcagc
     7921 gccttcagct ggtcggccgt gaacggcggc ttgtcgctga acagcgcata gccgagcagc
     7981 agccagcgga acagcgtgta cgggatgtgc acgatcggcg tcctcaggcc cttgacgcgc
     8041 ctgatcgtgt ggatgatgtc gacgtagtcg atgcgcgtgt cgccaacgat gtcgtaaatc
     8101 gcccggtccg gcgcgcgctc catgcactgg acgatgatcc tgcagaagtc gcggttatag
     8161 agcggctggc gcatgtagcg gccgtggccg gggatcggga acaccggcac cttcgccatg
     8221 aagcgcgaca gccagccgag gtgcttcggg tcgaaccagc cgaacatcag cgtcgggcgc
     8281 agcacgcagc acggcaggcc gctgtcgagc acgagctttt cctgcgcctt cttggtgttg
     8341 gtgtagtcgt catcggcgac cgagttgacc accgacgaac tgatgtgcac gagaaacggc
     8401 acctgatgct cgcgcacggc ttcgagcacg agcttcgtcg aatccaggtt gttgcgcacg
     8461 aacagctgcg cttccttgcc ggtgatctgc gcctgcagct gcagcacgcg cgccgcgccg
     8521 cgcaggtgtt cgcgccacgg gccgggctcg gcgaggtcgg ccagcaccgt ggtcacgtcc
     8581 ggatgcagct gcgccagcac gccgaggttg tgcgcgtgct tgtcgatcgc caccagctgc
     8641 cggtagccct gcgccttcag ctcgacgatc aggttctggc cgacgaggcc ggcggcgccg
     8701 agtacgacga tcttgtcgtt cttgtccatc gggatcggtt ccggtcggga atgcaggaaa
     8761 ggggagcttc ggcctacagg cggatgcgcc ggaacaccgg ctcggggatc gcgcggatga
     8821 tcgccatgat cggccgccag aagccgggca ggtagacgac actgctgcgc cgctcgatcg
     8881 cccggacgat gccggcggcg atcgtgtcgg cgctcgccca cagcggcccc ttcgggaagg
     8941 cggcggtcat cggcgtgtcg acgaagccgg gcttgatggt cagcacgttg acgccggcct
     9001 tcgcgagccg ctggcgcagg ccgccggtga aggccgtgac cagcgccttg gcgctgccgt
     9061 agacgtaatt gctctgccgg ccgcgatcgc cggccaccga cgagatcacc gccagcgtgc
     9121 cgtggcgctg gcgctcgaag cgcccggcaa gctcggtcag cagcgcgacg gtgctcaagc
     9181 cattggtgtg gatctcggcg agcgtctgtt cgaccgaggc ctcgcaggcc gcctggtcgg
     9241 acagcgtgcc gtgcgcgatc agcacggtgt cgagcccgcc gagtgcggcc gtcgcgcgat
     9301 cgagcatcgc tgcgtgcccg gcgacatcgt tggcatcgag cacctcaact gcgaccaatg
     9361 ctgccccgcg cacgcgcagg tcggcggcca gcgcttcgag ccgcccggcg cgccgtgcgc
     9421 acaggcagag cgcatcgcct tcggcggcaa agcggcgggc acaggcttcg gcaatcgccg
     9481 aggtcgcgcc cacgatcagg accttgcgca tcatcggctc ccggatgtgc cagtcattgc
     9541 ggttgcgtgt ccagccatcg tcaggccatg acccgacgcc agaagctcga cgagaactgc
     9601 gggtcgaggt aatcggcgaa acgctgccag cgcggatacg cggcccggaa cagcgcgccg
     9661 ctcatgcgcg cgtccttggc cggatagatc gcgccggcgg cctcgcggac caccgcatcg
     9721 agccggtcga gcagcgcgaa ggtggcggcg ccgcggttcg ggaaatccag cgccagcgtg
     9781 tagccggggc gcgggaacga cagccagccc ggcgaggcga gctcgccgaa gcgcttgagc
     9841 acggcgagga acgagccgct gccgctcgcg gcgatctgcg cgaggatcgc gtgcagcgca
     9901 tcgagcccgg cggccggcgg cagcgcgcac tgccactgca ggaagccgtg cggaccgtag
     9961 atgcggttcc agtcgccgat gccgtcgagc ggatagaaga acggctcgta atgcgtgatc
    10021 tgcgtgcatc cgcgtccgcc gaccggccgg tggtagtaca gcgcgttgaa tgcgtgcagc
    10081 gtcgggccgt tgatcagcga caccggcggc acgaagggca cgcgcagccg gccgggcttc
    10141 gccgccggcc gggttcgcgg cagggccggc gcgtggttgc cgcgcatgaa cagcccgcgt
    10201 ccggccgcgg ccgcgcagtc gatccacgcc accgtgtatt cgtagtcgtc atcggattcg
    10261 gccgacagcg catcgaactc ggcgagcgag ccgaagcgga tggtctcgcc ggcgatccac
    10321 ggattggcga tgcgccgcag ctgcagctcg acgcgcgtca ccagcccggt caggccgagg
    10381 ccgccgatgc tcgcgccgta ccagtcggcg tgttcgtgcg cgctgcacca gcggcggctg
    10441 ccgtccgagc gcagcagctc gaagccacgc aggtgccggc cgaagctgcc ggcgcggtgg
    10501 tgattcttgc cgtgcacgtc gttggcgagc gcgccgccga cggtcacgta gcgcgtgccc
    10561 ggcgtcaccg gcaggaacca gccctgcggc acggccagcg cgaggatatc ggcaagcagc
    10621 acgccagcct cgcatgcgag cacgccgctc tgcggatcga agccgatgaa gcggtccagc
    10681 ccgcgtgcga gcagcacgcc gccgccgtcg ttgaggcagg aatcgccgta gctgcggccg
    10741 ttgccgtagg gcagccagct cgtgtcgctc gccggcagcg gcgcatcgcg gtcggtgagc
    10801 ggccgggcat gctgcacgac gcgcggatac cggccccagg aggcggcgca ggcccgtgtc
    10861 gccgtcatac cgccgccagc acgatcagcc cggttgcgaa cagcagccag cggctggcgc
    10921 ggtcgcgcac cgcgaacacg accgggtcgt cgtgcatctc gtcgcggtgc gtcttcagcc
    10981 agacgcggct gacccagtac agcagcaccg gacacagcag ccagatcagc tccggccggg
    11041 cgtagagctg gcggctggtg tcgctgttga tgtacagcgc cagcaccagc acgctgttgt
    11101 aggccgaggc gatgccgagc gtctgcagca gcgccaggtc gtgcgtgctg tagtcgcgcc
    11161 cgctggcgcg ctcgcggccg cggcgcagca tctcgcgcag ctccgcatag cgcttgacca
    11221 gcgcgagact caggaacagg aacaccgaga acgccagcag ccagaaggag ttgtgcaggc
    11281 tgaccgccgc ggcgccggcg aggatgcgac tggtgtacag cccggcgagc gcgatcacgt
    11341 cgatcagcac gaactccttg agcttcaccg agtacgcgac cgtcagcgcg taatacagcg
    11401 tcagcatgcc gaagaactcc gccggcaggc cgaggcacag caggcccgcc agcgccagca
    11461 gcgccggaat cagcagcgcg ccgcgcacca gcccgagcgc gccgctcgcg aacggccgct
    11521 gacgcttgcg cggatgacgg cggtcggagg gcaggtcgag caggtcgttg agcagataga
    11581 cgctcgatgc acagaggccg aaggccagga aggcggtcag gctcgcggcg atggcgcgga
    11641 catcgatctg gtgactgagc agcagcggca ggaagatcag caggttcttc acccactggt
    11701 gcaggcgcag cgcctgcgcg tagaccttca ggcccgccgg gcgcggcgcg aactcgcgct
    11761 cgatcgtcgc cacccgcgcc agctccgggc gcagccgcgc cggcaggtcg acgctgatcg
    11821 cgtgccgcgc gtggcgccag acttcgaggt cgatcgcggc attgccggcg tagtcgtacc
    11881 cgcgctcgcc gagcgccgcg accagacgct cgcgcttgcg tgcgccggaa agattgagcc
    11941 cgtcctcact cgccagcacg cgctcgaaca gcccgaggtg ttcggccagc ggctcggcca
    12001 gcagccggtg cgaggccgtg cacagccaca gcggccggcc gcgcgcatgc tcggcgcgca
    12061 gccagtcgag gaaggcggtg tcatagggca gcagggcgta atcgggctgc acgcgccggg
    12121 cgatctcgta cttcagcacc gccttgccgc gccagagcca cagcggcacc aggaacaggc
    12181 acagcggccg gtgcttcagc agcaggaaga acgactcgac cagcaaatcg gaacggatca
    12241 gcgtgccgtc cagatccacg cacagcggga ttggcgtcaa ggagaacgac tccatcggtg
    12301 cagccgcagc cgtaccggac ggctgcggca ggcgccggtc gcgggggcgg acgggaacgg
    12361 tggcggatca cccggcaacg acgcgccggc gacgatcagg gcggagcgca gagtagccgc
    12421 gcgccgccac gagcggcaat cgacggatcg tgacatttgt tgcagcgcat gatggccgtc
    12481 gtccgtgatc gcgacgggcg cacggccagc gtccacctcg ggcgaccgac cgcagatcat
    12541 cgccagcaag cccgccgagt acctgca
//
LOCUS       DAOQIE010000078        12538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2452_length_12538_cov_7.753024, whole genome shotgun sequence.
ACCESSION   DAOQIE010000078 DAOQIE010000000
VERSION     DAOQIE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_12538_cov_7.753024"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PK144_06730"
     CDS             complement(<1..137)
                     /locus_tag="PK144_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517066.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HNF65457.1"
                     /translation="MNEFLPNFLPQLTQQLINGLSLGSIYALIAIGYTMVYGIIGMIN
                     FA"
     gene            complement(281..1402)
                     /locus_tag="PK144_06735"
     CDS             complement(281..1402)
                     /locus_tag="PK144_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein"
                     /protein_id="HNF65458.1"
                     /translation="MKLRQNLRRSVLVTAVAGALAALSAAASADIRIAIAGPITGPVA
                     QYGDMQMIGAEMAIERINAAGGIKGEKLVGVKMDDACEPKQAPTVANKVVNDGIKYVV
                     GHLCSGATKPASAIYDENDIVMVTPAATNDAITAGGMKTVFRTIGKDSQQGPTAGRFI
                     AATLKPKALAIIHDKQSYGQGIADQVRVTAEAAGIKPVMFEGINKGDTDFSALITKLK
                     QAGADVIYYGGYHPELGLLLRQAAEQGLKAKWMGPEGVGNKDLNAIAGDAVEGLYVTL
                     PQDFSSEPANAALVQAFKDKKQDPTGPFVLTSYAAVQVIADSIAAVGNDPKKVAEHLH
                     KSSFKTPIGELAFTASGDLKDFKFVVYEWHKDASKTPAQ"
     gene            complement(1657..1980)
                     /locus_tag="PK144_06740"
     CDS             complement(1657..1980)
                     /locus_tag="PK144_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNF65459.1"
                     /translation="MPFPTQIDQVSVPVKANVYFDGKVVSHTLLHADGSKQTLGLIQP
                     GEYRFSTGAPERMDIVAGNCRVRLAGLPDWSGYASGESFDVPGDSAFEIAVDDGLCEY
                     LCSFL"
     gene            complement(2116..5148)
                     /locus_tag="PK144_06745"
     CDS             complement(2116..5148)
                     /locus_tag="PK144_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65460.1"
                     /translation="MIRLPLAHKIAASVLPTVLVTVLGLGLWTYRTAYETARERIHVA
                     LGYVADRYLHDRVETIWARSWEAIDASQRARALSEAADIVPATGAGLQIVVLDEQDRV
                     LYLTGTGFAGAHADWARMAREAAAGQGTLGSSDGEWLYARRDFAPWGWRILCLMPEAS
                     VTEMLGGIRRATLTVAAVAGFVVVLLLLWLSGEVLLKPIGQLRAAAARIARSPTRLRI
                     GVPDGDELGELARAMEQMSSALAEQIDARSTAERSLERVKNLYAALSGANRAIVRVHE
                     RTELFRMLCRIAAEHGRFRLAWVGVPDEYGEMCPVASAGPASDILDGIRISIDEHLPE
                     GQGTMGRAWRSGGWAVENDFLGASVLRPWHRAARQHAIYAVAAFALREEGRTIACLSV
                     YAGERDFFRDDLIELLKDLATDVSFALDGIARAGERDRAEVARREAEERWHFALEGSG
                     DGVWDYDIPAGRIFASRRCMELLGHGDGDGVSDGYLEWEHLLHPEDRVPARLAIDAHL
                     RGETERFARDQRLLAADGRWRWFRTSGKVMRRDAEGRPLRMLGTLHDITAHKEAEARI
                     LWLGHRDALTGLPNRALLADRLEQALVHARRTGGRVAVMFLDLDRFKSINDSLGHEAG
                     DLLLKEVAARLCACVRGEDTVSRRGGDEFVVVLAEAESLADVDQIAARILARLAEPFA
                     LDGRIVHTSTSLGIALCPEDGSEAEMLIRNADAAMYSAKERGRNNFQFYTAGLNEQAA
                     RRLAIEAGLRAALAEGTLELFYQPQAELASGRVLRAEALLRDSRGRLADVSPAEFVPV
                     AEECGLIDRIGEWVLRAACQQARAWADAGLPLLPVAVNLSAAQFRRRDLAAIIADALA
                     GEALPGEWLEIELTESLVMRDAEAAAEIFRQLKAMGVRLAIDDFGTGYSSLSYLKRFP
                     IDRLKIDRSFVRDLPADRSSAELTRAIVAMAHALHLEVVAEGVERAEQQDFLAAIGCD
                     QYQGYWLAAPRSAADFAVWLAGQEVCGDAALPARRAAG"
     gene            complement(5272..6657)
                     /gene="hemN"
                     /locus_tag="PK144_06750"
     CDS             complement(5272..6657)
                     /gene="hemN"
                     /locus_tag="PK144_06750"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNF65461.1"
                     /translation="MESQIVIDERLIAKYDRAGPRYTSYPTAPQFHERFTAAHYRELA
                     LASNAGGRPLSLYFHIPFCDTVCFYCACNKIVTKNRRRAGPYLERLLREIELQGALFD
                     RSRPVVQLHWGGGTPTFLSEDEMRTLMEATRAHFTLLGDERGEYSIEIDPREADAGKI
                     ALLRRLGFNRISMGVQDFDPAVQLAVNRIQPKAVTLAAIDAARAEGFRSISLDLIYGL
                     PLQTVTSFARTLDEILELDPDRLSVFNYAHLPELFKTQKQIDAAALPSPAEKLEILRL
                     VIARLTAAGYVYIGMDHFAKPDDELAVAQRAGTLYRNFQGYSTHADCDLVAMGATSIG
                     MVGDSYSQNVKTLDAWYAHLDAGELPVYRGVHLDADDRLRRAVITQLLCNFALDYAPV
                     EAAHGIVFAEYFATELAELAGMAADGLLTLDRHGIRVAPAGRLLIRNICMVFDQYLRA
                     AGTQRRYSRVI"
     gene            complement(6755..7675)
                     /gene="prmB"
                     /locus_tag="PK144_06755"
     CDS             complement(6755..7675)
                     /gene="prmB"
                     /locus_tag="PK144_06755"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNF65462.1"
                     /translation="MTDLSLQSHLHTVRDFIRWSASRMTAEGVYFGHGTSNAIDEAAA
                     LVLHALHLPPDLHAAYFDANLVPDEKRAILNLLDQRVGQRIPLPYLTQRAWFMGLPFF
                     VDRRVLIPRSPLAELIERQFAPWVQAEKVSRILDLCTGSGCIGIACAHAFPEAEIDLA
                     DISTEALDVAAINVADHGVDDRVELIRSDLFSALAGRRYGLILSNPPYVGHDEMTALP
                     PEYAHEPRLALESGEDGLDAPLAILKQAREFLEDDGVLVVEVGNAQWALRERLPELPF
                     LWLEFDRGGEGVFLLSAAELDEHADAIAAA"
     gene            7817..8458
                     /locus_tag="PK144_06760"
     CDS             7817..8458
                     /locus_tag="PK144_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF65463.1"
                     /translation="MMRTRPLLLAFAGIAALAAGILLGDRLVGPPPDPLPSIHGTVIA
                     PPQPLPAFQLRDEHDTPFGPERFRGRWTLIYFGYTYCPDVCPTELTTLAELEKRLAAQ
                     GRGGDTDYWLITVDPERDTVQRLREYVQFFSKRFHAASGDTANLAALMNPLGVIALRP
                     PGQPDTGYTIDHSSTLTLIDPQGRLHAIFSPPNEVEHLLPELLALQARYAARP"
     gene            8489..9376
                     /gene="asd"
                     /locus_tag="PK144_06765"
     CDS             8489..9376
                     /gene="asd"
                     /locus_tag="PK144_06765"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229092.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNF65464.1"
                     /translation="MTDETPTLAERLRNSPQYWLPAAAITALTHRITRVRAPWLRKPL
                     IRWFIDAYRVNMAEAAEPDPEAYEHFNAFFTRALAAGARPIVAGEDRLCCPVDGAVSQ
                     AGALAGERVLQAKGHDYSLTALLGGEPADSAPFAGGCFATLYLSPRDYHRIHMPVDGR
                     LRTMIHVPGRLFSVSPLTTRCIPGLFARNERVVTLWDTAAGPMALVLVGAINVASMET
                     VWAGTITPPLGGTIRRWQYPVEGEGAVHLAKGDELGRFNMGSTVILAFAPGAVGLDAS
                     IRAGAPVRLGQTLAQRLVG"
     gene            9373..10233
                     /locus_tag="PK144_06770"
     CDS             9373..10233
                     /locus_tag="PK144_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65465.1"
                     /translation="MTAARSAAATLLMLALGGCTLAPAGEDHASNVGSVTMAPLMHAQ
                     TSPAASLAAFSPKYVTAVPHPRPSLSVPAVAAPLHLGYRSLAGGGEIGFHGRLPAPRF
                     GWFTRSQLELGAAVQGREQRLDARAAARLRLTHEPGNQRWRAGLELGSALRSRPALRA
                     HWTRRHDAGAFGTLTGGVLVASAALPNLPFERAPAANAFNGFWQPVLARAGRTALMIG
                     RLAWQAHSHWPLSGGSRLRPGLAVDFGDTGTHASLTLGVDTTLGRWSLGIEHDADGQP
                     QAGLRYLGAL"
     gene            10346..12538
                     /locus_tag="PK144_06775"
     CDS             10346..12538
                     /locus_tag="PK144_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF65466.1"
                     /translation="MMIRAVCCLLALLCASLPAAAAPEPRIGLVLSGGGARGLAHIGV
                     LRVLEELHVPVHAITATSMGSIVGGAYASGLGVDEMQARVLKIDWNDLFQDDPPREQR
                     PMRRRQDDRRPLVNATLGYGGGQFKLPKGAVNGQKLGIFLRQLVSNAEDIERFDALPV
                     PFRAVATDLVNGQMKVFDRGDLALAMRASMSVPSAFAPVEIDNHLYVDGGLTRNLPVD
                     IARQMGVDVIIAVNLGTPLLPREDLQSLLGVTVQMISILTEQNVQASLGQLDKRRDVL
                     VLPQLDGISSADFSKAREAIAAGEAATRAAAAQLRRYSIAPERWTAWSAARTQHRPPI
                     TQIDEVVVEGLERVNPAVARDVLNPPKGAGVGREELERHVGELYGYGDFERIDYRVRR
                     EGGKDLAIVDAVEKSWGPTYLRFGLGLSSEANGDATYGLRASALTTWINPLGAEWRTD
                     LLFGSTQSVGTEFYQPLVPGGAYYLAPWAEFRNRDMSIFVNDDRVARYRVKTLTAGLD
                     FGMTLGDRAELRFGAYRGTTRDDVDVGLTLFPEVSRDDGGLRLRLAYDTLDSLDFPHQ
                     GSRGLFEVTDSLGSFGAEQDYLRTQLDWTTAWTFGKGTLLATFSYGDTVSGRLPFDEQ
                     FVLGGIGRLSGYRTEQLRGERMLFGRLAYLQRLGEVARMPYYFGGTLEYGRMWQNARQ
                     ESILDQGQDHSSASLMFGIDSFLGPAFLSYGIGDDGQQNVYLLLGRPW"
BASE COUNT         1826 a         4395 c         4418 g         1899 t
ORIGIN      
        1 gcgaaattga tcatgccgat gatgccgtac accatcgtgt agccgatcgc gatcagcgcg
       61 tagatgctgc cgagcgacag cccgttgatc agctgctgcg tgagctgcgg cagaaagttc
      121 ggcaggaatt cgttcatggg cccgccccga tgtgctctcc cccggatgaa tcacgccccc
      181 gccggcgtgg ggggccggcg ggggcgtcgt gcgcaccggc cccggagcct ctgtagctcc
      241 ggccgggcgc gcatgccgat gcgctgcgct gtggttgcgt ttattgcgcc ggcgtcttgc
      301 ttgcgtcctt gtgccattcg tagacgacga acttgaagtc cttcaggtcg ccgctggcgg
      361 tgaaggcgag ctcgccgatc ggggtcttga agctgctctt gtgcaggtgc tcggcgacct
      421 tcttcggatc attgccgacc gcggcgatcg aatcggcgat cacctgcacg gcggcatacg
      481 aggtcagcac gaacggcccg gtcgggtcct gcttcttgtc cttgaaggcc tgcaccagcg
      541 cggcgttggc cggctcgctg gagaagtcct gcggcagcgt cacgtacagg ccctcgacgg
      601 catcgccggc gatggcgttg agatccttgt tgccgacgcc ctccgggccc atccacttcg
      661 ccttcaggcc ctgctcggcg gcctggcgca gcagcaggcc gagttcgggg tggtagccgc
      721 cgtagtagat gacgtcggcg ccggcctgct tgagcttggt gatcagcgcc gagaagtcgg
      781 tgtcgccctt gttgatgcct tcgaacatca ccggcttgat gccggcggcc tcggcggtga
      841 cgcgcacctg atcggcgatg ccctgaccgt aggactgctt gtcgtggatg atcgccagcg
      901 ccttcggctt gagcgtggcg gcgatgaagc gtccggcggt cgggccctgc tgactgtcct
      961 tgccgatggt gcggaacacc gtcttcatgc cgccggcggt gatcgcgtcg ttggtcgcgg
     1021 ccggcgtgac catgacgatg tcgttctcgt catagatcgc cgaggccggc ttggtcgcac
     1081 ccgagcacag gtggccgacc acgtacttga tgccgtcgtt gacgaccttg ttggcgacgg
     1141 tcggggcctg cttcggctcg caggcgtcat ccatcttcac gccgacgagc ttctcgccct
     1201 tgatgccgcc ggcggcgttg atgcgctcga tggccatctc ggcgccgatc atctgcatgt
     1261 cgccgtactg cgcgaccggg ccggtgatcg ggccggcgat ggcgatgcgg atgtcggcgc
     1321 tggccgcggc actcagcgcg gcgagcgcac cggcgacggc ggtgacgagc acgctgcggc
     1381 gcaggttctg gcgaagcttc atgggcgatc tcctctgcga aacgaacgaa ggtccgggca
     1441 gcgcgaagtc ggattcaggc tgcgcgcgtc agacggcaaa cacgggcatc gggcgcggca
     1501 gccgccgcgc gggcggcgca agagtggtcc gacaaccgcc cggactcaag ccaccgggcg
     1561 gaagccgggc gccacagtat ggaaacagga atgaacgctg ccggccttga tcggtgtcag
     1621 cgcgaccggt cgcggtgaag gcgacgacgg cggcggttac aggaaactgc acaggtattc
     1681 gcagagaccg tcgtcgacgg cgatctcgaa ggccgaatcg ccgggcacgt cgaagctctc
     1741 gccgctcgcg tagccgctcc agtccggcag gccggccagc cgtacgcggc agttgccggc
     1801 gacgatatcc atgcgctcgg gcgcgccggt gctgaagcgg tattcgccgg gctggatcag
     1861 gccgagcgtc tgtttactgc catcggcgtg cagcagcgtg tggctgacga ccttgccgtc
     1921 gaaatagacg ttggccttga ccggcacgct gacctggtcg atctgggtcg ggaacggcat
     1981 cggggatgct cctggctggg gcgcacgcgc gcggaaaaaa cacggaagga aacgcgcagg
     2041 gatcggcatg caccggcgtg gtacggaccg gaccccggtg cgggcgcggg agcttagcgc
     2101 agcggccggc gcgcttcagc cggccgcgcg ccgggccggc agcgcggcat cgccgcaaac
     2161 ctcctgcccc gccagccaga cggcgaagtc agcggccgag cgcggcgcgg ccagccagta
     2221 gccctggtac tggtcgcagc cgatcgccgc cagaaaatcc tgctgctcgg cgcgctcgac
     2281 gccttcggcg acgacttcga ggtgcagcgc gtgcgccatc gccacgatcg cgcgcgtcag
     2341 ctcggcactg gagcgatcgg cgggcaggtc gcgcacgaag ctgcggtcga tcttcagccg
     2401 gtcgatcggg aaacgcttga gataggacag cgacgaataa ccggtgccga agtcgtcgat
     2461 cgccagacgc acgcccatcg ccttcagctg gcggaagatc tcggcagcgg cctcggcatc
     2521 gcgcatgacc aggctctcgg tcagctcgat ctccagccac tcgccgggca gggcttcgcc
     2581 ggccagcgca tcggcgatga tcgcggcgag gtcgcgccgg cggaactgcg cggccgacag
     2641 gttcacggcc accggcagca gcggcagccc ggcatcggcc caggcgcgcg cctgctggca
     2701 ggcggcgcgc agcacccatt ccccgatgcg gtcgatcagc ccgcactcct cggcgaccgg
     2761 cacgaactcg gccggcgaca catcggcgag ccgcccgcgg ctgtcgcgca gcagcgcctc
     2821 ggcgcgcagc acgcgaccgc tcgcgagctc ggcctgtggc tgatagaaca gctccagcgt
     2881 gccctcggcg agcgcggcgc gcagccccgc ctcgatcgcc agccggcgcg cggcctgctc
     2941 gttgaggccg gccgtgtaga actggaagtt gttgcgcccg cgttccttgg ccgaatacat
     3001 cgccgcatcg gcgttgcgga tcagcatctc ggcctcgctg ccgtcctcgg ggcagagcgc
     3061 gatgccgagg ctggtcgagg tgtgcacgat gcgcccgtcg agcgcgaacg gttcggcgag
     3121 gcgcgcgagg atgcgcgcgg cgatctggtc gacatcggcg agcgactcgg cctcggcgag
     3181 cacgacgacg aactcgtcgc cgccgcggcg gctgaccgta tcctcgccgc gcacgcaggc
     3241 gcagaggcgc gcggcaacct ccttcagcag caggtcgccg gcctcgtgcc cgagcgaatc
     3301 gttgatgctc ttgaagcggt cgaggtcgag gaacatcacc gccacgcgcc cgccggtgcg
     3361 ccgcgcatgc accagtgcct gttcgaggcg gtcggcgagc agcgcacggt tcggcaggcc
     3421 ggtcagcgcg tcgcggtggc cgagccacag gatgcgggcc tcggcctcct tgtgcgcggt
     3481 gatgtcgtgc agcgtgccga gcatccggag cggccggcct tcggcgtcgc ggcgcatgac
     3541 cttgcccgag gtccggaacc agcgccagcg gccgtcggcc gcgagcaggc gctggtcgcg
     3601 cgcgaagcgc tcggtctcgc cgcgcaggtg cgcgtcgatc gccaggcgcg ccggcacgcg
     3661 gtcctcgggg tgcagcaggt gctcccactc cagatagccg tcggaaacgc cgtcgccgtc
     3721 gccgtggccg agcagttcca tgcagcggcg cgacgcgaag atgcgcccgg ccggaatgtc
     3781 gtagtcccag acgccgtcgc cgctgccttc gagcgcgaag tgccagcgct cctcggcctc
     3841 gcgccgcgcc acctcggcgc ggtcgcgctc gccggcgcgg gcgatgccgt cgagcgcgaa
     3901 ggacacgtcg gtggcgagat ccttcagcag ctcgatcagg tcgtcgcgga agaagtcgcg
     3961 ctcgccggcg tagacgctca ggcaggcgat cgtgcggcct tcctcgcgca gcgcgaaggc
     4021 cgcgaccgcg tagatcgcgt gctggcgcgc ggcgcgatgc cagggccgca gcaccgaagc
     4081 gccgaggaag tcgttctcga ccgcccagcc gccgctgcgc caggcccggc ccatcgtgcc
     4141 ctggccctcg ggcaggtgct cgtcgatcga gatgcggatg ccgtcgagga tgtcgctcgc
     4201 cggaccggcg ctcgcgaccg ggcacatctc gccgtattcg tcgggcacgc cgacccaggc
     4261 cagccggaag cgcccgtgct cggcggcgat gcggcagagc atgcggaaca gctcggtgcg
     4321 ctcatgcacg cgcacgatcg cgcggttggc accggagagc gccgcgtaca ggttcttgac
     4381 gcgctccagg ctgcgctcgg ccgtgctgcg cgcgtcgatc tgctcggcga gggccgagga
     4441 catctgttcc atcgcgcgcg ccagttcgcc gagttcgtcg ccgtcgggca cgccgatgcg
     4501 cagccgcgtc gggctgcggg cgatgcgcgc ggccgcggcg cgcagctggc cgatgggctt
     4561 gagcagcacc tcgccggaga gccacagcag cagcagcacg acgacgaagc cggcgacggc
     4621 ggcgacggtc agcgtcgcgc ggcggatgcc gccgagcatc tcggtgacgc tcgcctccgg
     4681 catcaggcag aggatgcgcc agccccaggg cgcgaagtcg cggcgcgcat acagccattc
     4741 gccgtcggac gagccgagcg tgccctggcc ggcggcggcc tcgcgcgcca tgcgtgccca
     4801 gtcggcgtgc gccccggcga agccggtgcc ggtcaggtac agcacgcggt cctgctcatc
     4861 gagcacgacg atctgcaggc cggcgccggt ggccggcacg atgtcggcgg cctcgctgag
     4921 cgcccgggcg cgctggctgg cgtcgatcgc ctcccagctg cgggcccaga tcgtctcgac
     4981 tcggtcatgc aggtagcgat cggcgacgta gccgagcgcg acgtggatgc gctcgcgcgc
     5041 ggtctcgtag gcggtgcggt aggtccagag cccgaggccg agcaccgtga ccagcacggt
     5101 cggcagcacc gaggccgcga tcttgtgcgc gagcggcagg cggatcacgg cggcgatccg
     5161 ccgtgcggct gcggatggaa tgtcggcaag gctccctctc ccctgcggcg ctctggcggc
     5221 gcctgcacgg ccgtcccgag tcaggcgggt caaccggatc agacgacccg atcagatgac
     5281 gcgagaatac cggcgctgcg tacccgctgc acgcaggtat tgatcgaaaa ccatgcagat
     5341 gttgcggatc agcaggcggc cggccggcgc gacgcggatg ccgtgccgat cgagcgtgag
     5401 caggccgtcg gcggccatgc cggcaagctc ggcgagttcg gtcgcgaaat actccgcgaa
     5461 gacgatgccg tgggcagcct cgaccggcgc gtaatcgagc gcgaagttgc agagcagctg
     5521 cgtgatcacg gcgcggcgca ggcggtcatc ggcatcgagg tgcacgccgc ggtagaccgg
     5581 cagctcgccg gcatcgaggt gcgcgtacca ggcgtccagg gtcttgacgt tctggctgta
     5641 ggaatcgccg accatgccga tcgaggtcgc gcccatcgcc accaggtcgc agtcggcgtg
     5701 cgtgctgtag ccctggaagt tgcggtacag cgtgccggcg cgctgcgcga cggcgagttc
     5761 gtcgtcgggc ttcgcgaagt gatccatgcc gatgtacacg taaccggccg cggtcaggcg
     5821 ggcgatcacg agcctgagga tttcgagctt ctcggccggc gacggcagcg ccgcggcatc
     5881 gatctgcttc tgggtcttga acagctccgg caggtgcgcg tagttgaata ccgacagccg
     5941 gtccggatcg agttcgagga tctcgtcgag cgtgcgcgcg aagctcgtga ccgtctgcag
     6001 cggcaggccg tagatcagat cgaggctgat cgagcggaaa ccctcggcgc gcgccgcgtc
     6061 gatcgccgcg agcgtcaccg ccttcggctg gatgcggttg acggcaagct gcacggccgg
     6121 gtcgaagtcc tgcacgccca tgctgatgcg gttgaagccg agccggcgca gcagcgcgat
     6181 cttgccggcg tcggcctcgc gcggatcgat ctcgatcgag tactcgccgc gctcatcacc
     6241 gagcagcgtg aagtgcgcgc gcgtcgcctc catcagcgtg cgcatctcgt cttcggataa
     6301 aaaggtcggc gtgccgccgc cccagtgcag ttgcacgacc gggcgcgagc gatcgaacag
     6361 cgcgccctgc agctcgatct cgcgcagcag ccgttcgaga tacggcccgg cgcggcggcg
     6421 gttcttcgtg acgatcttgt tgcaggcgca gtagaagcag accgtgtcgc agaacgggat
     6481 gtggaagtac agcgacagcg gccggccgcc ggcattgctc gcgagcgcga gctcccggta
     6541 gtgcgcggcc gtgaagcgct cgtgaaactg cggcgcggtc gggtaggagg tgtagcgcgg
     6601 cccggcgcgg tcgtacttcg cgatcaggcg ctcgtcgatg acgatctggg attccatcgg
     6661 tcggtccgga aagaggctcg tgcggggtga agcggcgcag cctggcgcag cgcgcgaccg
     6721 gcggccttga tccactgccg ggcggcgggt gttttcaggc cgcggcgatc gcgtcggcgt
     6781 gctcgtcgag ttcggccgcg ctcagcagga acacgccctc gccgccacga tcgaattcga
     6841 gccacaggaa cggcagctcc ggcaggcgct cgcgcagcgc ccactgggcg ttgccgacct
     6901 cgaccaccag cacgccgtcg tcttcgagga actcgcgcgc ctgcttcagg atcgcgagcg
     6961 gcgcatcgag gccgtcctcg cccgattcga gcgcaagccg cggctcgtgc gcgtattcgg
     7021 gcggcagcgc cgtcatctcg tcgtggccga cgtagggggg gttgctcagg atcagcccgt
     7081 agcgccgccc ggccagcgcc gagaacagat ccgagcggat cagctcgacg cgatcgtcga
     7141 cgccgtgatc cgcgacgttg atcgccgcca catcgagcgc ctcggtcgag atgtcggcga
     7201 gatcgatctc cgcctccgga aaggcatgcg cgcaggcgat gccgatgcag ccgctgccgg
     7261 tgcagagatc gaggatgcgc gagacttttt cagcctgcac ccagggcgcg aactggcgct
     7321 cgatcagctc ggcgagcggc gagcgcggaa tcagcacgcg ccggtcgacg aagaacggca
     7381 ggcccatgaa ccaggcgcgc tgcgtcaggt acggcagcgg aatgcgctgg ccgacgcgct
     7441 ggtcgagcag gttcaggatc gcgcgcttct cgtcgggcac gaggttggcg tcgaaatacg
     7501 cggcatgcag gtccggcggc aggtgcagcg catgcagcac cagcgccgcc gcctcgtcga
     7561 tcgcgttgct ggtcccgtgg ccgaagtaca cgccttcggc cgtcatgcgg ctcgcactcc
     7621 agcggatgaa atcgcgcacg gtatgcaggt gggattgcag gctgaggtcg gtcatcgctg
     7681 cgcactccgc ggaaaaggcc gcgactatat caccgcctcg tggcaccggc cggacgcgca
     7741 cgaccatgcg ctacactgcg ccgcttcctg ccgacccggc cgccgcgcct gcccgccctc
     7801 cggagtgatg cccgacatga tgcgcacgcg cccgctgctc cttgccttcg ccggcattgc
     7861 cgccctcgcc gccggcatcc tgctcggtga tcgtctcgtc ggcccgccgc ccgatccgct
     7921 gccgagcatc cacggcaccg tgatcgcgcc gccgcagccg ctgccggcct tccagctgcg
     7981 cgacgaacac gacacgccct tcggaccgga acgcttccgc ggtcgctgga cgctgatcta
     8041 cttcggctac acgtattgcc ccgatgtctg cccgaccgag ctgaccacgc tcgccgagct
     8101 cgaaaagcgg ctggcggcgc agggcagggg cggcgacacc gactactggc tgatcacggt
     8161 cgatcccgag cgcgacaccg tccagcgcct gcgcgagtac gtgcagttct tcagcaagcg
     8221 cttccacgcc gccagcggcg acaccgccaa cctcgcggcg ctgatgaatc cgctcggcgt
     8281 catcgcgctg cgtccgccgg gacagccgga caccggctac acgatcgatc attcctcgac
     8341 gctgacgctg atcgacccgc agggccggct gcacgcgatc ttctcgccgc cgaacgaggt
     8401 cgagcacctg ctgcccgaac tgctggcgct gcaggcccgc tacgccgccc gtccctgaac
     8461 gcccgccgcc tccggacccg ccaccccgat gaccgacgaa accccgacgc tcgccgagcg
     8521 cctgcgcaac agcccgcagt actggctgcc ggccgccgcg atcacggcgc tgacccaccg
     8581 catcacgcgc gtgcgcgcgc cgtggctgcg caagccgctg atccgctggt tcatcgacgc
     8641 ctaccgcgtc aacatggccg aggccgccga gcccgacccc gaggcctacg agcatttcaa
     8701 cgccttcttc acgcgcgcgc tggccgccgg tgcccgcccg atcgtcgccg gcgaggaccg
     8761 gctctgctgt ccggtcgatg gtgccgtcag ccaggccggt gcgctcgccg gcgagcgcgt
     8821 gctgcaggcc aagggtcatg actattcgct gacggcgctg ctcggtggcg aacctgccga
     8881 cagcgcgccg ttcgccggcg gctgcttcgc gacgctgtac ctcagtccgc gcgattacca
     8941 ccgcatccac atgccggtcg acggccgcct gcgcacgatg atccacgtgc cgggccggct
     9001 gttctcggtg tcgccgctga ccacgcgctg catccccggt ctgttcgcgc gcaacgagcg
     9061 cgtcgtgacg ctctgggaca ccgccgccgg cccgatggcg ctggtgctgg tcggggcgat
     9121 caacgtcgcg tcgatggaaa ccgtctgggc cggcacgatc acgccgccgc tcggaggcac
     9181 gatccgccgc tggcagtatc cggtcgaagg cgaaggcgca gtgcatctgg cgaagggcga
     9241 tgaactcggg cgcttcaaca tgggctcgac cgtgatcctc gccttcgcgc ccggcgccgt
     9301 cggcctggac gcctcgatcc gtgccggcgc gccggtgcgc ctcggtcaga cactggcgca
     9361 gcggctggtc ggatgacggc cgcccgaagc gccgctgcga cgctgctgat gctggcgctc
     9421 ggcggctgta cgctggcccc ggccggggaa gatcatgcgt cgaacgtcgg cagcgtcacc
     9481 atggcaccgc tgatgcatgc gcagacgagt ccggccgcct ccctcgcggc gttcagcccg
     9541 aagtacgtca ccgccgtgcc gcatccccgc ccgtccctgt ccgtgccggc cgtggccgcg
     9601 ccgctgcact tggggtatcg cagcctcgcc ggcggagggg agatcggttt ccacggccgg
     9661 ctgccggcgc cgcgcttcgg ctggttcacc cgctcgcaac tcgaactcgg agccgccgtg
     9721 cagggccgcg agcaacggct cgatgcccgt gccgctgccc ggctgcgcct gacgcacgag
     9781 ccgggcaacc agcgctggcg cgccggactt gaactcggca gcgcgctgcg cagccgcccg
     9841 gcgctgcggg cacactggac ccggcgccac gacgccggtg cgttcggcac gctgaccggc
     9901 ggtgtgctgg tggcaagcgc tgcgctcccg aacctgccgt tcgagcgcgc accggctgcg
     9961 aacgcattca acggcttctg gcagccggtg ctggcccggg ccggccggac ggcactgatg
    10021 atcggccggc tcgcctggca ggcgcacagc cactggccgc tgtccggcgg cagtcgtctg
    10081 cggcccggcc tggccgtgga tttcggcgat accggcaccc atgcgagcct gacgctcggc
    10141 gtcgacacca cgctcgggcg ctggtcgctc ggcatcgagc acgatgccga cggccagccg
    10201 caggccgggc tgcgctatct cggtgctttg taatccggcg taatccggcg cttgccagcg
    10261 ccggcgcggc ttcggcatcg tcgcagccct gaaaccgggc ggccgcaagc tgccccgtcc
    10321 ccgcttccgc ttcgagtttc cgccgatgat gatccgagcc gtctgctgcc tgctcgcgct
    10381 gctgtgcgca tccctgcccg ccgccgccgc ccccgaacca cgcatcggac tggtgctctc
    10441 cggcggcggc gcgcgcggac tcgcgcacat cggcgtgctg cgcgtgctgg aagagctgca
    10501 cgtgccggtg catgcgatca cggcgacctc gatgggctcg atcgtcggcg gggcctacgc
    10561 ttcgggcctc ggcgtcgacg agatgcaggc gcgcgtgctg aagatcgact ggaacgacct
    10621 gttccaggac gatccgccgc gcgagcagcg gccgatgcgc cggcgtcagg acgaccgccg
    10681 gccgctggtc aacgccacgc tcggctacgg cggcggccag ttcaagctgc ccaagggtgc
    10741 ggtgaacggc cagaagctcg gcatcttcct gcgccagctc gtcagcaacg ccgaggacat
    10801 cgaacgcttc gatgcgctgc cggtgccgtt tcgcgccgtg gccaccgatc tcgtcaacgg
    10861 ccagatgaag gtgttcgacc gcggcgacct ggcgctggcg atgcgcgcgt cgatgtcggt
    10921 gccgagcgcc ttcgcgccgg tcgagatcga caatcatctg tatgtcgacg gcggcctgac
    10981 ccgcaacctg ccggtcgaca tcgcgcgcca gatgggcgtc gacgtgatca tcgccgtcaa
    11041 cctcggcacg ccgctgctgc cacgcgagga cctgcagtcg ctgctcggcg tcacggtgca
    11101 gatgatcagc atcctgaccg aacagaacgt gcaggcgagc ctcggccagc tcgacaagcg
    11161 ccgcgacgtg ctggtgctgc cgcagctcga cggcatcagc tcggcggact tcagcaaggc
    11221 gcgcgaagcc atcgccgccg gcgaggccgc cacgcgcgcc gcggccgcgc agcttcgccg
    11281 ctacagcatc gcacccgagc gctggacggc gtggtcggcg gcgcgcacgc agcatcgccc
    11341 gccgatcacg cagatcgacg aggtcgtcgt cgaggggctg gagcgcgtga atccggccgt
    11401 cgcccgcgac gtgctgaatc cgccgaaggg tgccggggtc gggcgcgagg agctcgaacg
    11461 tcacgtcggc gagctgtacg gctacggcga cttcgagcgc atcgactacc gcgtgcgccg
    11521 cgagggcggc aaggatctgg cgatcgtcga tgcggtcgag aaatcctggg gaccgacgta
    11581 tctgcgcttc ggcctcggcc tgtcctcgga ggccaacggc gatgccacct acggcctgcg
    11641 tgcctcggcg ctgaccacgt ggatcaaccc gctcggggcc gagtggcgca ccgatctgct
    11701 gttcggctcg acgcagtcgg tcggcaccga gttctatcag ccgctggtgc cgggcggcgc
    11761 gtactacctg gcgccgtggg cggaattccg caaccgcgac atgtcgatct tcgtcaacga
    11821 tgaccgtgtc gcgcgctatc gcgtgaagac gctgaccgcc ggtctcgact tcggcatgac
    11881 gctcggcgat cgcgccgagc tgcgcttcgg tgcctaccgc ggcacgaccc gcgatgatgt
    11941 cgatgtcggc ctgacgctgt tcccggaagt cagccgcgac gacggcggcc tgcgcctgcg
    12001 cctggcctac gacacgctcg acagcctcga cttcccgcac cagggctcgc gtggcctgtt
    12061 cgaagtcacc gattcgctcg gcagcttcgg cgccgaacag gattacctgc gcacgcagct
    12121 cgactggacc acggcctgga ccttcggcaa gggcacgctg ctcgcgacct tctcctacgg
    12181 cgatacggtc tccggcagac tgccgtttga cgagcagttc gtgctcggcg gcatcggccg
    12241 cctgagcggc tatcgcaccg agcagctgcg cggcgagcgc atgctgttcg ggcgactggc
    12301 ctatctgcag cgcctcggcg aggtcgcacg catgccgtat tacttcggcg gcacgctcga
    12361 atacgggcgc atgtggcaga acgcccgtca ggaatcgatc ctcgatcagg ggcaggacca
    12421 cagctcggcc agcctgatgt tcggcatcga ttccttcctc ggcccggcct tcctgagcta
    12481 cggcatcggc gatgacgggc agcagaatgt gtatctgctg ctggggaggc cgtggtga
//
LOCUS       DAOQIE010000079        12507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2463_length_12507_cov_6.409573, whole genome shotgun sequence.
ACCESSION   DAOQIE010000079 DAOQIE010000000
VERSION     DAOQIE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2463_length_12507_cov_6.409573"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..617)
                     /locus_tag="PK144_06780"
     CDS             complement(21..617)
                     /locus_tag="PK144_06780"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNF65467.1"
                     /translation="MPVVRSALLRPFVLAGALAALNAPAADAPAPAEADLAAARAVVQ
                     QFAAELKNALGSAIQAGGPVNGIEVCQQQAPGIATRAAQQAGFTRVGRTSLKVRNPDN
                     APDAWELAGLEDFEARKAAGADPATLERAGIVALPGGGHEFRYLKAIPTAAVCLNCHG
                     STLKPEVSAKIDALYPSDAARGFAEGDLRGAFTLRKPL"
     gene            complement(733..3786)
                     /locus_tag="PK144_06785"
     CDS             complement(733..3786)
                     /locus_tag="PK144_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65468.1"
                     /translation="MSPVSRLPRSPAVAFRWLLLGCSLLLPGFAAAAAEDSWPREFGR
                     NGRSVLVYEPQVEDWSADGRLQLRAPVAVTPARGEPARYGVLGIASPTEVDAGSQSVL
                     LGAPEVSAQFPDLPADEAARLQALVREALPAGRPLTLALPTLIAAGGRPPAAAASEVA
                     VDLDPPPIFHSTRPATLLMFYGPPAFEPVGDSPLQAATNTESTVLYEPDAQRYWLALE
                     DGWLTAPDLRAGPWTQPASLSPAFRSLPADAWGALTARGPGPRMTRAPAVIYSERPAE
                     LIVTDGPPRYVPVAGTRLEAVANTDSPVFVLPVARRHYFLTAGRWFTAPTLAGPWQPA
                     TTDLPPDFTQIPPNGPYGWVLASVPGTPAAAEAVALAGVPRKSTVPRQGLRYAPVYEG
                     PPRYEPIDGTGGVDWIVNTPYEVVRYEDRYYGVDDGVWFSADEPGGPWYPAERVPGAI
                     YRIPPAHPLYHVTYVRIYGHTPETVTYGYTPGYTGQYVSNGVLMFGVGALVGWALADR
                     GDRHPDHIPYRPWYRPAQPWRAYGYGAVYRPGKGFAPRGWAWGRYRPKPLPVAWTGHE
                     KRWARSPLLIEQRRVTTTTVLSAGAAARTRVVELPGTPFAGGDRYRRWGGRSPALDER
                     WQQARRFEAARPAPAKLRRQGPMFVGQDGALYRRSARDDWQRVAGDGRSIPVDRALAT
                     RQIAAPAKGPGARGSDALRRGQALVPAQNPADRPDRADRPDRGPDRREPGPARFEAGR
                     GGADPQRRDEAGAQAPAVREPGARPDRDPSGRDAPPDRRQEAAPRSPDRDARARAQAP
                     DDGPDSPARDGRPQPRPEAGSPERPQATGRDERPQPRPEARPPERPQATGRDERPQPR
                     SEARPPERPQAAGRDERPQPRPEARSPERPQAAGRDERPQPRAEARPPERPQPAVRVD
                     RPQPRAEVRPPERPQPRAEARPSERPQPAARVERPQPRPEARPPERPQPAARAERPQP
                     RQAPPPPARVERPRPEARQAAPAPQPGGGGGGGKGHGGREDKGKG"
     gene            complement(3897..7187)
                     /locus_tag="PK144_06790"
     CDS             complement(3897..7187)
                     /locus_tag="PK144_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65469.1"
                     /translation="MARIAVVDDLDFNRDLVAALLRPAGHDIVEAADGAAALEMVRRE
                     RPELIVCDVLMPVMDGYEFVRRLRSDPVCGEYGARVPVIFWSAYYNEDEALHLAAECG
                     VSLLLPKPCPPERLQAVIRQALSEQPPPPLPVPDETGFEGQHRRLLTDKLSEQTTALL
                     RANARLTALLGMVLALGSEHDPQRLLAAACRGARELLGASDAWLVYREDDSAPVRSVS
                     DGPHSALPAPAGFGGSSSQPQDPRFGAVLVAPLSSMRTCRGWLMIARLPGEAPFEDED
                     EHLLGLLAAQTLHAWEIERLNGQLSAHAAALEDEVAERERITRQLDVQYAIARQLAGA
                     QGIDAVLPVLLRTLGERLGFEIAALWLPDARHGGLRCEQVWAAPGSAHLGCAAARIHR
                     RLAPEKGQPGRSRLQRRPLWSLDLHRDEDSGRDPDCPDLHACATLPIAAGERIVAVFE
                     LLAGERRPPDPGLLDLMWAIGEQVGQFWARIDQQHRNARLARVHAVLSGINGALVRIH
                     ERAALLRATCRIAVEQGEFGIAWIGDYDAAAARVTPVAWAGIDDGFGAQALALASSDD
                     DYAALIVREAIHRREAVWCNDLAAEAAPVHSRFAEAQRRGFGSLIVLPLVVDHRIGGV
                     LALYTQAPDYFTREERELLEQVASDIAFGLADIDRRDRLDYLNHYDPLTGLPNRTLWR
                     ERLGHALIESARHHERTAVLVLDIARFRHVNNGFGRAAGDALLRALAERAAAVWPEPA
                     HVGRLGADCFAGFLPRLADVQAITQLLEGRLGAALSAPFPVDGHELRPGFVAGIAIAP
                     DDGDEAETLLHHAEAALAQAKASDRPYVYYRPELTSGIAGTLRLENRLHRALERGEFE
                     LHYQPRADARSGRLTGLEALLRWRDPEAGLVAPLRFVPVLERLGLINAAGEWAIRRAL
                     ADSRRWTAVAGPLRVAVNVSAVQLREPGLGDTICSALAEAPPDGAPLDLEITESVLMS
                     QVEVHGQMLASLRRRGVGIAIDDFGTGYSSLAYLARLPVDALKIDRSFVETLTDRPES
                     LAIVRTVISLAQSLHLKTVAEGVETAEQAGRLAELGCDELQGFWISRPLPLDALLDWL
                     RARTPAGV"
     gene            complement(7208..7597)
                     /locus_tag="PK144_06795"
     CDS             complement(7208..7597)
                     /locus_tag="PK144_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65470.1"
                     /translation="MNHPNILIVEDNQANFAVLDYLLRAHGFHVLAAGNGREALDVLA
                     REPVDLVLCDMQMPVMDGYEFARCTRANPRYARLPLLAVTASSMAGDRTRVLAAGFDD
                     YFSKPIDPETFVDAMEHHLPPALRRAR"
     gene            complement(7594..9522)
                     /locus_tag="PK144_06800"
     CDS             complement(7594..9522)
                     /locus_tag="PK144_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF65471.1"
                     /translation="MTDTDRWTEANPDALIGLTAAGVVQHWSAGAQRQYGYPAHEALG
                     RPLEALIVPADMTGQQQWMLAETLALELCAFEAVRRRRDGSLVCVDITARRIAGEDGE
                     SAVLFVEKDVTRLKILRDARLMEARFRDLLESTPDAVVIAGTTGHIVIANAQAERLFG
                     YSPGELRGQPVEVLLPARYRAAHVSHRAGYFQQPRRRSMGAGLELYGLRRDGTEFPVE
                     ISLNPLRTEAGQMVMSAIRDISERQRAERKFRGLLEAAPDAMVIVDRDGRIVLANAQT
                     ATLFGFERRELLGERVERLLPPRFRDRHPAHRNAYFTDPKLRPMGTGFELYGLRRDGT
                     EFPVEISLSPLETEDGLLVSAAIRDITERKRFERALQDKNSELEAANRAKSRFLASMS
                     HELRTPLNAVLGFTGILLMKLPGPLNPEQESQLRTVKSSAQHLLALINELLDLARIEA
                     GRVVLKPEPVALATLLDEVAATLRTQADDKGLALGVELEAVPEPVTDRRLLLQILINL
                     AGNAVKFTQQGSVRLRAGPGRPDSRGHATVCIAVADTGPGISPEEQKRLFEPFAQGEA
                     ARSSGIESSGLGLHLSRELAGVLGGWLQCRSIPGEGSTFTLTLPLHAELPAAADLPGA
                     TAPAELRQPASSGGDRPA"
     gene            9883..10728
                     /locus_tag="PK144_06805"
     CDS             9883..10728
                     /locus_tag="PK144_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65472.1"
                     /translation="MSVTDPTPPPAASSREARLAELIELHARDFRGHPYLPEGCAPFT
                     LEAGRVVGHYHGLRLQTTLLALLDAGGRPVARLAQTAAFGAGGQPVSAWAPYAIALDD
                     AAITYLDRLIRTVHVLNAGSRPGGGPLWLAVHPLHLAAVPDAHGAVFASILRRSGLRP
                     DGIVLELADAARVAPQRLAAALAGFRSRGYGVAFGFNGSDPAELDRLLALAPDAIRLG
                     APHLRAAAASEAEQQRLRARIEHIHRHRVRVWLPAAADAREAALLPSLGADGWQAADA
                     SGSLP"
     gene            10725..11279
                     /locus_tag="PK144_06810"
     CDS             10725..11279
                     /locus_tag="PK144_06810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF65473.1"
                     /translation="MSGACPDDGLQILIVPGLGDSGPGHWQTLWADSGPAFRRVHQRD
                     FDQPQREAWIAGLEAELRRRPGPTVLVGHSLGCIAIAAWAQRHDCAQVAGALLVAPAD
                     ADALGLLDGVPLAPLPFPATVVASSDDPYCTLQRARAFAAAWAAEFIDHGPSGHLNVA
                     AGFGAWPRGERLLAGLRARVMAGV"
     gene            11359..11796
                     /locus_tag="PK144_06815"
     CDS             11359..11796
                     /locus_tag="PK144_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF65474.1"
                     /translation="MSIVDITTDEGRIAEPGWLARAEEVHRELRPQLPEEYVEKMQRV
                     FAGGARMSVAVDDDAVLGVAVWRVYEDTSIGRKLYVDDLVTTESARSEGVGQTLLTWL
                     EARARKLDCRALTLDSGVQRARAHRFYFREGLSVFAFNFRRDL"
     gene            complement(11774..>12507)
                     /locus_tag="PK144_06820"
     CDS             complement(11774..>12507)
                     /locus_tag="PK144_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HNF65475.1"
                     /translation="DEVRQVLDGLRDDIAGTVRVSVPVSLGETCFDDIVVGFCRQYPR
                     VHIALDLSNGIRDLVADGIDFALRSGSEPAPDLIARPLYSMEEYACASPAYLAAAGTP
                     LAPADLAAHACLQNLHFGEEAAAGEWEFHRGAELWRVPVRGPLACNHYSLLKKAAIAG
                     AGIARLPGYLIDDELADGRLLRLLPDYESRRLPIFLVYAPQRTLPQRTRVLIDYVREW
                     FAGKAQRCAARRAALAVNLTDPGGS"
BASE COUNT         1637 a         4499 c         4673 g         1698 t
ORIGIN      
        1 ccgctgcggc aaggccggcc tcagagcggc ttgcgcagcg tgaacgcgcc gcgcaggtcg
       61 ccttcggcga agccgcgcgc ggcatcggag gggtacagcg catcgatctt cgcgctgacc
      121 tcgggcttca gcgtgctgcc gtggcagttc aggcagacgg cggcggtcgg gatcgccttc
      181 aggtagcgga actcgtggcc gccgccgggc agggccacga tcccggcgcg ttcgagcgtc
      241 gccgggtcgg caccggcggc cttgcgcgcc tcgaagtcct ccagcccggc gagttcccag
      301 gcatcgggcg cgttgtcggg attgcggacc ttcaggctgg tgcgaccgac gcgcgtgaag
      361 ccggcctgtt gcgcggcgcg ggtggcgatg cccggggcct gctgctggca gacctcgatg
      421 ccattgaccg gcccgccggc ctggatcgcg ctgccgagcg cgttcttcag ctcggccgcg
      481 aactgctgga cgacggcgcg ggcggcagcg agatcggcct cggccggtgc cggcgcatcg
      541 gcggccgggg cgttgagggc ggccagggcg ccggcgagga cgaagggacg aaggagggcg
      601 gagcggacga cgggcatcgg tgacctcggc agacttcggg acggcgcgca cggcggcgcg
      661 ggcgtgatcc tcaagtctgt cggggccggg agccgggcgc actgacgatc cgcagccgcg
      721 cccggccgtg attcagccct tgcccttgtc ctcacgcccg ccgtggccct tgccgccacc
      781 accgccaccg cccggttgcg gcgcaggggc ggcctggcgg gcttccggac gcggacgctc
      841 gacacgcgcg ggcggcggcg gtgcctgccg gggctgcgga cgttcggcgc gggccgccgg
      901 ttgcggacgc tcgggcggcc gtgcttcggg acgtggctgc gggcgctcga cgcgggctgc
      961 cggttgcgga cgctcggacg gccgggcttc tgcgcgtggc tgtggacgct cgggcggccg
     1021 gacttccgca cgtggctgcg ggcgatcgac gcggaccgcc ggttgcggac gctcgggcgg
     1081 tcgggcctct gcacgcggct gcggacgttc atcccgaccc gccgcttgcg gccgttccgg
     1141 cgaccgggct tcgggacgcg gttgcggacg ttcgtcccga cccgccgctt gcggacgttc
     1201 cggcggccgg gcttcggaac gcggctgcgg acgttcatcg cgacccgtcg cttgcggacg
     1261 ttccggcggc cgggcttcgg gacgcggttg cggacgttcg tcgcgacccg tcgcttgcgg
     1321 gcgttccggc gacccggctt cgggacgcgg ctgcggacgc ccgtcgcggg ccggcgaatc
     1381 cgggccatcg tccggggcct gcgcccgggc ccgcgcgtcc cggtccggcg agcggggcgc
     1441 cgcctcctgc cggcgatccg gcggcgcatc gcggccggac gggtcacgat ccgggcgcgc
     1501 gccgggctcc cggactgccg gtgcctgcgc tccggcctcg tcgcgacgct gcgggtccgc
     1561 gccgccgcga ccggcctcga accgggccgg tccgggctca cgccggtccg ggccgcgatc
     1621 cgggcgatcc gcacgatccg gacgatccgc agggttctgg gcgggcacca gcgcctgccc
     1681 gcggcgcagc gcatcgctcc cgcgcgcgcc cggccccttc gccggagccg cgatctggcg
     1741 cgtggcgagt gcgcgatcga ccgggatgct gcggccgtcg ccggcgacgc gctgccagtc
     1801 gtcgcgcgcc gagcgccggt acagcgcgcc gtcctgaccg acgaacatcg gcccctgccg
     1861 gcgcagcttc gccggggccg ggcgggccgc ctcgaagcgg cgggcctgct gccagcgttc
     1921 atcgagcgcg gggctcctgc cgccccagcg ccggtagcgg tccccgccgg cgaacggcgt
     1981 gcccggcagc tcgacgacgc gggtgcgcgc cgcagcgccg gccgacagca ccgtcgtcgt
     2041 cgtcacccgc cgctgttcga tcagcagcgg cgagcgcgcc cagcgcttct cgtgaccggt
     2101 ccaggccacc ggcagcggtt tcggccggta gcgcccccag gcccagccgc gcggcgcgaa
     2161 gcccttgccg ggccggtaca cggccccgta gccatacgcg cgccagggct gcgccggccg
     2221 ataccacggc cggtaaggga tgtggtccgg gtggcggtcg ccacgatcgg ccagtgccca
     2281 gccgaccagc gccccgacgc cgaacatcag cacgccgttg ctgacgtact ggccggtgta
     2341 gcccggcgtg tagccgtagg tgacggtctc gggcgtgtgc ccgtagatgc gcacgtaggt
     2401 gacgtgatag agcggatgcg ccggcgggat gcggtagatc gccccgggca cgcgctcggc
     2461 cggataccac ggcccgccgg gctcgtcggc ggagaaccag acgccgtcat cgacgccgta
     2521 gtagcgatcc tcgtagcgca cgacctcgta cggcgtgttg acgatccagt cgacgccgcc
     2581 ggtgccgtcg atcggctcgt agcgcggcgg accctcgtag accggcgcgt agcgcaggcc
     2641 ctggcgcggc acggtcgact tgcgcggcac gccggcgagc gccacggctt cggccgccgc
     2701 cggcgtgccc ggcaccgagg cgagcaccca gccgtacggc ccgtttggcg gaatctgcgt
     2761 gaagtccggc ggcagatcgg tcgtcgccgg ctgccacgga ccggccagcg tcggtgccgt
     2821 gaaccagcgc ccggcggtga ggaagtaatg ccggcgtgcc acgggcagca cgaacaccgg
     2881 cgagtcggtg ttggcgacgg cttcgagccg cgtgccggcg accggcacgt agcgcggcgg
     2941 gccgtcggtg acgatcagct cggccgggcg ctcgctgtag atcacggccg gcgcgcgcgt
     3001 catgcgcggc cccggaccgc gcgcggtcag cgcgccccag gcatcggccg gcagcgaccg
     3061 gaacgccggc gacaggctgg ccggctgcgt ccacggcccg gcacgcagat ccggggccgt
     3121 cagccagccg tcctcgagcg ccagccagta gcgctgcgca tccggctcgt acagcaccgt
     3181 cgattcggtg ttggtcgccg cctgcagcgg cgagtcgccg accggctcga aggccggcgg
     3241 gccgtagaac atcagcagcg tcgccggccg cgtgctgtgg aagatcggcg gtggatcgag
     3301 atcgaccgcc acctcggacg cggcggcggc cggcggccgg ccgccggcag cgatcagtgt
     3361 cggcagcgcg agcgtcagcg gccgcccggc cggcagcgcc tcgcgcacca gcgcctgcag
     3421 gcgggcggcc tcgtcggccg gcaggtccgg gaactgcgcg ctgacctcag gcgcgccgag
     3481 cagcaccgac tgcgagccgg catcgacctc ggtcggtgac gcgatgccga gcacgccgta
     3541 gcgcgccggc tcgccgcgcg cgggcgtgac cgccaccggc gcgcgcagct gcaggcggcc
     3601 gtcggcggac cagtcctcga cctgcggctc gtagaccagc accgaacggc cgttgcggcc
     3661 gaactcgcgc ggccaggaat cctcggcggc agcggccgcg aaaccaggca gcagcaggct
     3721 gcagccaagc agcagccagc ggaatgcgac cgcgggcgag cgcggcaaac gggaaacagg
     3781 ggacatctgc gcatcctcga tccgcacgag cgggcgtcac gaccctgacc cgggccaccg
     3841 gtgcggatgc ggctgcggcc cgcgattcgg agtacgggaa cggcgcggaa gttgcgtcag
     3901 acgccggccg gcgtgcgggc gcgcagccag tcgagcagcg catcgagcgg cagcggccgg
     3961 ctgatccaga agccctgcag ctcgtcgcag ccgagttcgg cgagccgccc ggcctgttcg
     4021 gcggtctcga cgccttcggc gacggtcttc aggtgcagcg actgcgcgag cgagatcacg
     4081 gtgcgcacga tcgcgaggct ctcggggcga tcggtcagcg tctcgacgaa gctgcggtcg
     4141 atcttcagcg catcgaccgg caggcgcgcc agatacgcga gcgaggaata gccggtgccg
     4201 aagtcgtcga tcgcgatgcc gacgccgcgc cggcgcaggc tggcgagcat ctggccatgc
     4261 acctcgacct ggctcatcag cacgctctcg gtgatctcca gatcgagcgg tgcgccgtcg
     4321 ggcggtgcct cggcgagggc gctgcagatc gtgtcgccga gcccgggctc gcgcagctgc
     4381 acggccgaga cgttgaccgc gacgcgcagc ggcccggcga ccgccgtcca gcgccggctg
     4441 tcggcgaggg cgcggcggat cgcccattcg ccggccgcgt tgatcaggcc gagccgttcg
     4501 agcaccggca cgaagcgcag cggcgcgacc agcccggcct ccggatcacg ccagcgcagc
     4561 agcgcctcca gtccggtcag acgaccgctg cgcgcatcgg cgcgcggctg gtagtgcagc
     4621 tcgaactcgc cgcgctccag cgcccggtgc aggcggtttt cgagccgcag cgtgccggcg
     4681 atgccgctgg tcagctcggg gcggtaatac acgtagggcc ggtcgctggc cttggcctgc
     4741 gccagcgccg cctcggcgtg gtgcagcagg gtttcggcct cgtcgccgtc gtccggggcg
     4801 atggcgatgc cggcgacgaa gcccgggcgc agctcgtgac cgtcgaccgg gaacggtgcc
     4861 gacagcgcgg caccgagccg gccttcgagc agctgcgtga tcgcctgcac atcggcgagc
     4921 cgcggcagga agccggcgaa gcagtcggcg ccgagccgcc cgacgtgcgc gggctcgggc
     4981 cagaccgccg cggcgcgctc ggcgagcgcg cgcagcagcg catcgccggc ggcgcggccg
     5041 aagccgttgt tgacgtgccg gaagcgggcg atgtcgagca cgagcacggc cgtgcgctcg
     5101 tggtggcgcg cgctctcgat cagcgcgtgc ccgagccgct cgcgccacag cgtgcggttc
     5161 ggcaggccgg tcagcggatc gtagtggttc aggtagtcga gccggtcgcg gcggtcgatg
     5221 tcggcgaggc cgaaggcgat gtcgctcgcg acctgttcga gcagctcgcg ttcctcgcgc
     5281 gtgaagtagt ccggcgcctg cgtgtacagc gccagcacgc cgccgatgcg gtgatcgacg
     5341 accagcggca gcacgatcag cgagccgaag ccgcggcgct gcgcctcggc gaagcggctg
     5401 tgcaccggtg cggcctcggc ggcgagatca ttgcaccaga ccgcctcgcg ccggtggatg
     5461 gcctcgcgca cgatcagcgc ggcgtagtca tcgtcgctgc tggcaagcgc gagcgcctgc
     5521 gcgccgaaac cgtcatcgat gccggcccag gccaccggcg tcacccgtgc ggcagcggcg
     5581 tcgtagtcac cgatccaggc gatgccgaac tcgccctgtt cgacggcgat gcggcaggtg
     5641 gcgcgcagca gcgccgcgcg ctcgtggatg cgcaccagcg cgccgttgat gccgctcagc
     5701 accgcgtgca cgcgcgcgag gcgggcgttg cgatgctgct ggtcgatgcg ggcccagaac
     5761 tggccgacct gctcgccgat cgcccacatc aggtcgagca gacccgggtc cggcggccgg
     5821 cgctcgccgg ccagcagctc gaacaccgcg acgatgcgct cgccggcggc gatcggcagc
     5881 gtcgcgcagg cgtgcaggtc agggcagtcc ggatcgcggc cgctgtcctc gtcgcggtgc
     5941 aggtcgaggc tccagagcgg ccggcgctgc aggcggctgc gccccggctg gcctttctcc
     6001 ggcgcgagcc gccggtggat gcgggccgcg gcgcagccga ggtgcgccga gccgggcgcg
     6061 gcccagacct gctcgcagcg caggccgccg tggcgggcat cgggcagcca cagcgcggcg
     6121 atctcgaaac cgaggcgctc gccgagcgtg cgcagcagca ccggcagcac ggcatcgatg
     6181 ccctgcgcac cggcgagctg acgggcgatc gcgtactgca cgtcgagctg gcgggtgatg
     6241 cgctcgcgct cggcgacctc gtcctcgagc gcggcggcat gcgccgagag ctggccgttc
     6301 aggcgctcga tctcccaggc atgcagcgtc tgcgccgcga gcaggccgag caggtgttcg
     6361 tcctcgtcct cgaacggcgc ctcgcccggc aggcgggcga tcatcagcca gccgcggcag
     6421 gtgcgcatcg agctgagcgg ggcgaccagc accgcgccga agcgcggatc ctgcggctgg
     6481 ctgctgctgc cgccgaaacc ggccggtgcc ggcagcgccg agtgcggacc gtcgctgacc
     6541 gagcgcaccg gggcgctgtc gtcctcgcgg tacaccagcc aggcgtcgct cgcgccgagc
     6601 agttcgcggg cgccccggca ggcggcggcg agcaggcgct gcggatcgtg ttcgctgccg
     6661 agcgcgagca ccatgccgag cagcgcggtc aggcgggcat tggcgcgcag cagcgccgtg
     6721 gtctgttcgg agagcttgtc ggtcagcagc cgccggtgct gcccctcgaa tccggtttca
     6781 tcgggcaccg gcagcggcgg cggcggctgc tcgctgagcg cctgccggat caccgcctgc
     6841 aggcgctcgg gcgggcaggg cttgggcagc agcaggctga cgccgcattc ggcggccagg
     6901 tgcagggcct cgtcctcgtt gtaatacgcg ctccagaaga tcaccggcac gcgcgcgccg
     6961 tattcgccgc agaccggatc ggagcgcagc cggcgcacga actcgtagcc gtccatgacc
     7021 ggcatcagca cgtcgcagac gatcagttcc gggcgctcgc gccggaccat ctccagggct
     7081 gccgcaccat cggcggcctc gacgatgtcg tgcccggccg ggcgcagcag cgccgcgacc
     7141 aggtcgcggt tgaagtcgag atcatcgacg actgcaatgc gcgccatcgg ccgcccgccc
     7201 gcgcccgtca acgcgcccgt cgcagtgccg gcggcaggtg gtgctccatc gcgtcgacga
     7261 aggtttccgg atcgatcggc ttcgagaagt aatcatcgaa gccggccgcg agcacgcgcg
     7321 tgcgatcgcc ggccatcgac gaggcggtca cggccagcag cggcaggcgc gcgtagcgcg
     7381 ggttggcgcg cgtgcagcgc gcgaactcgt agccgtccat gaccggcatc tgcatgtcgc
     7441 agagcaccag atcgaccggc tcgcgcgcca gcacgtcgag cgcctcgcgg ccgttgccgg
     7501 cggcgagcac gtgaaagccg tgcgcccgca gcaggtagtc gagcacggcg aaattggcct
     7561 ggttgtcctc gacgatcagg atgttcggat ggttcatgcg ggccggtctc cacctgatga
     7621 tgcgggctgg cgaagctcgg ccggggccgt ggcgccgggc aggtcggcgg cggccggcag
     7681 ctcggcgtgc agcggcagcg tcagcgtgaa ggtgctgccc tcgccgggga tgctgcggca
     7741 ctgcagccag ccgccgagca cgccggccag ttcccggctc aggtgcaggc cgaggccgga
     7801 gctctcgatg ccgctgctgc gggcggcctc gccctgcgcg aagggttcga acagccgctt
     7861 ctgttcttcg ggcgagatgc cgggaccggt gtcggcgacg gcgatgcaga cggtggcgtg
     7921 ccccctggaa tcgggccggc cggggccggc gcgcaggcgc accgagccct gctgcgtgaa
     7981 cttgacggca ttgccggcga ggttgatcag gatctgcagc agcagccggc gatcggtgac
     8041 gggttcgggc accgcttcga gctcgacgcc cagggcgagc cccttgtcgt cagcctgcgt
     8101 gcgcagcgtc gccgcgacct cgtcgagcag cgtcgcgagc gccaccggct cgggcttgag
     8161 caccacgcgc ccggcctcga tgcgtgccag gtcgagcagc tcgttgatca gcgcgagcag
     8221 gtgctgggcg ctggacttga ccgtgcgcag ctgcgattcc tgctccggat tcagcgggcc
     8281 gggcagcttc atcagcagga tgccggtgaa gccgagcacg gcattgagcg gcgtgcgcag
     8341 ctcatggctc atgctggcga ggaagcggct cttggcacgg ttggcggctt cgagctcgct
     8401 gttcttgtcc tgcagcgcgc gttcgaagcg cttgcgctcg gtgatgtcgc ggatcgccgc
     8461 cgacaccagc aggccgtcct cggtctcgag cggactgagg ctgatctcga ccgggaattc
     8521 ggtgccgtcg cggcgcagcc cgtagagctc gaagccggtg cccatcggcc gcagcttcgg
     8581 atcggtgaag taggcgttgc ggtgcgccgg gtggcgatcg cggaagcgcg gcggcagcag
     8641 gcgctcgacg cgctcgccga gcagctcgcg ccgctcgaag ccgaacagcg tcgcggtctg
     8701 ggcgttggcg agcacgatgc ggccgtcgcg atcgacgatg accatcgcat ccggcgcggc
     8761 ttcgagcagg ccgcggaact tgcgctcggc gcgctggcgc tcgctgatgt cgcggatcgc
     8821 gctcatgacc atctgcccgg cctcggtgcg cagcgggttc aggctgatct cgaccgggaa
     8881 ctcggtgccg tcgcggcgca ggccgtagag ctcgagcccg gcgcccatcg agcgccggcg
     8941 cggctgctgg aagtagccgg cgcggtggct gacgtgcgcg gcgcggtagc gtgccggcag
     9001 cagcacctcg accggctggc cgcgcagctc gccgggcgag tagccgaaca ggcgctcggc
     9061 ctgggcattg gcgatgacga tgtggccggt ggtgccggcg atgaccacgg catccggggt
     9121 cgattcgagc aggtcgcgaa agcgcgcctc catcagccgc gcatcgcgca ggattttcag
     9181 gcgcgtcacg tccttctcga cgaacagcac cgccgattcg ccgtcctcgc cggcgatgcg
     9241 ccgcgcggtg atgtcgacgc agaccagcga gccgtcgcgg cgccggcgca cggcctcgaa
     9301 ggcgcagagc tcgagggcca gcgtctcggc cagcatccac tgctgctggc cggtcatgtc
     9361 ggccggcacg atcagcgcct ccagcgggcg cccgagcgcc tcgtgggcgg gatatccgta
     9421 ctggcgctgc gcgccggcgc tccagtgctg caccactccg gccgccgtca gcccgatcag
     9481 tgcatcgggg ttggcttcgg tccagcgatc ggtatcggtc atctgcatct cggggggcct
     9541 gtctcggtac atgatgccgc cggtgatccc gtccaccggc caatccgcaa cacccgcaga
     9601 cccgactccg gaatccctgt tccggccggt tgcgggttcc ggctcgcgtc atgcctcgtg
     9661 cctgcgtctg gcgcttcgag tctagtgcca tttgcaggcg gcatgccggg atggaaaatc
     9721 cttcggttgc agcgcagcag tgataagtca aatgtcgcag ataaataacc atgcgtaata
     9781 aaaagataat tttttattac tatatattat tgccaaaccg accgtaagct gactccgaat
     9841 ctccaatttt tcatgtttgt catcccgagc ggagccgccc agatgtccgt caccgatccg
     9901 accccgcccc cagcagcctc cagccgcgag gcacggctcg ccgagctgat cgaactgcat
     9961 gcccgtgact tccgcggcca tccctacctg ccggaaggct gcgcgccgtt cacgctcgaa
    10021 gccgggcgcg tggtcgggca ttaccacggc ctgcgcctgc agacgacgct gctggcgctg
    10081 ctcgatgccg gcgggcgccc ggtcgcgcgc ctcgcgcaga ccgccgcctt cggtgccggc
    10141 ggccagccgg tgtcggcctg ggcgccgtat gcgatcgcgc tcgacgatgc cgcgatcacc
    10201 tatctcgacc ggctgatccg caccgtgcac gtgctgaacg ccggcagccg ccccggcggc
    10261 gggccgctgt ggctggcggt gcatccgctg cacctcgccg cggtgcccga tgcgcacggg
    10321 gcggtgttcg catcgatcct gcgccgcagc ggcctgcgcc cggacgggat cgtgctcgaa
    10381 ctcgccgatg ccgcgcgcgt cgcgccccaa cggctggcag ccgcgctcgc cggcttccgc
    10441 agccgcggct acggcgtcgc gttcggcttc aacggcagcg atccggccga gctcgatcgc
    10501 ctgctcgcgc tcgcgcccga tgccatccgc ctcggtgccc cgcacctgcg tgcggctgcc
    10561 gccagcgagg ccgagcagca gcgcctgcgg gcacgcatcg aacacatcca tcgtcaccgg
    10621 gtacgcgtgt ggctgccggc ggcggccgat gcccgcgaag ccgcgctgct gccgagcctc
    10681 ggcgccgacg gctggcaggc ggcggacgca tccgggagcc tgccatgagc ggcgcgtgtc
    10741 cggacgacgg cctgcagatc ctgatcgtgc ccggcctcgg cgattccggt cccggccact
    10801 ggcagacgct gtgggccgac tccggcccgg ccttccggcg cgtgcaccag cgcgatttcg
    10861 accagccgca gcgcgaggcg tggatcgccg ggctcgaagc cgaactgcgc cgccggcccg
    10921 gtccgaccgt gctggtcggc cacagcctcg gctgcatcgc gatcgcggcc tgggcgcagc
    10981 gccacgactg cgcgcaagtc gccggtgcgc tgctggtcgc gccggccgat gccgatgcgc
    11041 tcggcctgct cgatggcgtg ccgctcgcgc cgctgccgtt cccggcaacc gtggtcgcga
    11101 gcagcgacga tccgtactgc acgctgcagc gcgcccgcgc cttcgcagcg gcctgggcgg
    11161 cggagttcat cgaccacggc ccgagcgggc acctgaacgt cgccgccggc ttcggagcgt
    11221 ggccgcgcgg cgagcggctg ctcgccgggc tgcgcgcgcg cgtgatggcc ggcgtctgac
    11281 gctggacttc cggccccgct tgccggcacc gtgcggcaaa actgctgacg gcgccggagg
    11341 ccgctcgaaa tcatgggcat gagcatcgtg gacatcacca ccgacgaggg caggatcgcc
    11401 gagccgggct ggctcgcgcg tgccgaggag gtccaccgcg agctgcgccc gcagctgccg
    11461 gaggaatacg tcgagaagat gcagcgcgtg ttcgccggcg gcgcccgcat gtcggtcgcg
    11521 gtcgatgacg acgccgtgct cggcgtcgcg gtctggcgcg tctacgagga cacctcgatc
    11581 ggccgcaagc tctacgtcga tgatttggtc acgaccgaga gcgcacgctc cgaaggcgtc
    11641 ggccagaccc tgctgacctg gctcgaagcc cgcgcccgca agctcgactg ccgggcgctg
    11701 acgctcgatt ccggcgtgca gcgcgcccgc gcccaccgct tctacttccg cgaggggctg
    11761 tcggtgttcg cgttcaactt ccgccgggat ctgtgaggtt cacggcgagc gccgcgcgcc
    11821 gggccgcgca gcgctgggcc ttgccggcga accattcgcg cacgtaatcg atcagcacgc
    11881 gcgtgcgctg cggcagcgtg cgctgcggcg cgtagaccag gaagatcggc agccgccgcg
    11941 attcgtagtc cggcagcagc cgcagcagcc ggccgtcggc gagttcgtca tcgatcagat
    12001 agcccggcag ccgcgcgatg ccggcgccgg cgatggccgc cttcttcagc agcgaataat
    12061 ggttgcacgc gagcggcccg cgcaccggca cgcgccagag ctcggcgccg cggtgaaact
    12121 cccattcccc ggccgcggcc tcctcgccga agtgcaggtt ctgcaggcag gcgtgcgcgg
    12181 cgagatcggc cggtgccagc ggcgtgccgg ccgcggccag atacgccgga ctcgcgcagg
    12241 cgtattcctc catgctgtac agcgggcgcg cgatcaggtc cggggccggc tcgctgcccg
    12301 agcgcagcgc gaagtcgatg ccgtcggcga ccagatcgcg gatgccgttc gacaggtcga
    12361 gcgcgatgtg cacgcgcggg tactgccggc agaagccgac gacgatgtcg tcgaagcagg
    12421 tctcgccgag cgacaccggc accgacacgc gcacggtgcc ggcgatgtca tcgcgcaggc
    12481 cgtcgagcac ctgacgcacc tcgtcat
//
LOCUS       DAOQIE010000080        12506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2464_length_12506_cov_7.536262, whole genome shotgun sequence.
ACCESSION   DAOQIE010000080 DAOQIE010000000
VERSION     DAOQIE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_12506_cov_7.536262"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="odhB"
                     /locus_tag="PK144_06825"
     CDS             <1..774
                     /gene="odhB"
                     /locus_tag="PK144_06825"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNF65476.1"
                     /translation="AAPAPAAKPAATPAALPAGAWPFSKRMEQRVPMTRLRARIAERL
                     LEAKNTTAMLTTFNEINMKPVIDLRNKYKDQFEKSHGVKLGFMSFFVKAVVESLKRYP
                     AVNASIDGNDIVYHDYYDVGIAVSSPRGLVVPILRNAEDMSLADIEKAIGDFGKRAKE
                     GSLTVEEMTGGTFTITNGGVFGSLMSTPILNPPQSGILGMHATKDRAVVENGQVVARP
                     MMYVAHSYDHRIIDGKEAVGFLVAVKEAIEDPARMLLSV"
     gene            886..2310
                     /gene="lpdA"
                     /locus_tag="PK144_06830"
     CDS             886..2310
                     /gene="lpdA"
                     /locus_tag="PK144_06830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF65477.1"
                     /translation="MAKTYDVVVIGGGPAGYVAAIRCAQLGLATACVEKWINIEGKPA
                     LGGTCLNVGCIPSKALLESSEHYHKLQHGLEAHGISVGEASVDVAKMIGRKEQIVSQL
                     TGGIAQLFKANKVDWLQGRGKLLAERQVEVTGHDGAVEVVEADNVIIAAGSVPIDIGA
                     APKDDKLIVDSTGALAFTEVPKRFGVIGAGVIGLELGSVWKRLGAEVVILEAVEDFLA
                     AADHQIAKDALRQFQKQGLDIRLGARVTGSTKTDTEVTVQYTDKNGNHELTFDKLLVS
                     VGRRPATDGIADAAADLVLNERGFIHVDDECRTNLPGVWAIGDCVRGPMLAHKGSEEG
                     IMVAEFIAGNHGHMNYDTIPWVIYTQPEIAWVGKTEQQLKHEGVPVKIGTFPFAANGR
                     AKAMDAAAGMVKIIAHAETDRVLGAHICGPMASELIQELVVAMEFGASAEDLQMTIHG
                     HPTLAEAVHEAALSVDKRALHRVN"
     gene            2477..3220
                     /locus_tag="PK144_06835"
     CDS             2477..3220
                     /locus_tag="PK144_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF65478.1"
                     /translation="MSASTRPVAVVTGANRGLGFETSRQLARRGYLVVVTSRHPEAGE
                     VASERLQAAAAEGGGEIVLQPLDVTRPDSIAALGAFVQARCGRLDALVNNAGVLLDGH
                     GLDDATGSSVFAASLETLRTTMETNVHGPLLLAQEFVPLMRRRGYGRIVNVSSGMGQL
                     ADMGGQWPAYRLSKTALNALTRILAAETARTGILVNAVCPGWARTDMGGPKAERTPEE
                     AASGIVWAATLPDDGPSGGFFRDGKPIPW"
     gene            3269..3655
                     /locus_tag="PK144_06840"
     CDS             3269..3655
                     /locus_tag="PK144_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNF65479.1"
                     /translation="MVPALLCLLGCQLLGEALVQLTRAPVPGPVLGMLLLALWLGLVA
                     RGRIPAALGQTADVLLAHLSLLFVPAGVGVMLHLQRIAAEWLAIVAALVLSTLLAIAA
                     TAIAMRAVLRRLPPPADDSDGRDAHG"
     gene            3642..4373
                     /locus_tag="PK144_06845"
     CDS             3642..4373
                     /locus_tag="PK144_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNF65480.1"
                     /translation="MPMDRNLLDIWVYLSAQPLLWLAVTLAAYLAGYRLFMASHQRAW
                     LNPVALAILLIAGLLAVTGTPYARYFEGAQFVHFLLGPATVALAVPLVRHWARIRRMW
                     LPLAVGLVAGSLTAALSAMAIAWGLGASLRTVLSMAPKSVTTPVAMGIAEKIGGLPSL
                     TAVLVILTGIVGAVLGPGLLARLGIREDEVRGYALGVAAHGIGTARAFQLGSLCGAFA
                     GLAIGLNALLTALLVPLLASMLGLS"
     gene            4536..6230
                     /locus_tag="PK144_06850"
     CDS             4536..6230
                     /locus_tag="PK144_06850"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNF65481.1"
                     /translation="MPRISARLPAVAALALLAACTVTPGVISGPRGPAALPPDTVWLD
                     QNWSDEDRDLWWFTGQGSHMIPYDWFLALPGADGKPFRADPTLAGYGFLTTPATVRNP
                     DALPVGFVKDQRPATDIRGQWLGMTCAACHTAQLRIKGVEVRVDGGPGLVDFDGFMNG
                     MVAAMDRAAASPDAFSRFAAEVLGSNATPEKLADLRTAFGEHTQFLKTRIAANAIPRP
                     AGHGRVDAFGQIFNQAVVHALGVPGNAHPADAPVSYPFIWDAPQHDRLQWNGSALNLG
                     AGPLARNSGEVVGVFGTVNVNQPGFGMRGYASSLDTQQLDGLERLLTTLWSPEWPSKY
                     AGPLDATLVARGKTLFAANCASCHQNIDAKGSVTFDRADPNRRITAQMIDLGKVGTDP
                     HMAKNVLACTADTGPLNGRPVAVLPFGPKLKPKDLCVNVTGNVVVGAILGNNPPVSLF
                     QVLEFLKGSQALKAKLMNPQYKARPLNGIWATAPYLHNGSVPTLADLLQPPAQRPKTF
                     HVGDREFDAGKVGYVTDRGPFVYDTAEPGNSNAGHLYGTKLATADKRALLEYLKSL"
     gene            6369..7841
                     /gene="astD"
                     /locus_tag="PK144_06855"
     CDS             6369..7841
                     /gene="astD"
                     /locus_tag="PK144_06855"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HNF65482.1"
                     /translation="MTHLIGSDWQPGGGESFASENPATGAMLWRGHAADAADVDAAVR
                     AARTAFPAWAALDFEARVERLRAFEATLQANAPELAGTISAETGKPRWETATEVAAMI
                     AKIGISIAAHAERTGTREGTAAGVRAVLRHRPHGVVAVFGPYNFPGHLPNGHIVPALL
                     AGNTVVFKPSEQTPLVAERTVQLWQASGLPAGVLNLVHGARATGQALATHPELDGLFF
                     TGSHATGEWLHRSFAGRTGRILALELGGNNPLIADAVTLSDLDAACWHIVQSAWITAG
                     QRCTCARRLIVPLGARGDALIERLCQWLDGIRIGAPDDEPEPFMGPLISNAAADALLN
                     FQTDLELAGARVLRRAQRLDPARPLLTPALLDVSACSLRPDAEAFGPLLQLVRVADFE
                     TALWEANHTRFGLAAGLLSDDATRWQQFRTTIRAGIVNWNRPTTGAASSAPFGGIGDS
                     GNLRPSAYYAADYCAYPVASLEADTLTRPAQAGTGLVPPA"
     gene            8170..8565
                     /locus_tag="PK144_06860"
     CDS             8170..8565
                     /locus_tag="PK144_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65483.1"
                     /translation="MPIVIASSFADPADIAAYKKAIAAGKTEAEALKVGDNGIGKWGD
                     DTTREDVPICALPPEDWEGKWGKGDTARGKKVAVTYKGKTVIGELRDTMPRKANIKNG
                     AGIDLNPGFAKAFGVRQPFMLKDVTWEWA"
     gene            complement(8697..9749)
                     /gene="arsB"
                     /locus_tag="PK144_06865"
     CDS             complement(8697..9749)
                     /gene="arsB"
                     /locus_tag="PK144_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNF65484.1"
                     /translation="MASVSRRLSFLDRWLTLWIFAAMAIGVAWGHFFPGIAGFWQGLS
                     SGTTSWPIAIGLIVMMYPPLARVRYEALPQVFRNTRVLLLSLLQNWVIGPLLMFALAV
                     LLLPDQPHYMAGLILIGLARCIAMVIVWNELARGDTEYCAGLVAFNALFQVLFFPVYA
                     WLFLSVLPGWLGLETFKVEVSIGEVAWTVFVYLGIPFLAGFVTRLVLRRMKGDDWYRT
                     VFVPRISPLTLIALLFTILVMFSLKGELIVRLPLDVLRIAVPLAIYFVAMFLLSFALG
                     RRYGAPYGQTATLSFTAASNNFELAIAVAVAVFGLNSGEAFAAVIGPLVEVPALIGLV
                     HVAGWLQRRWFVAAVA"
     gene            complement(9763..10287)
                     /locus_tag="PK144_06870"
     CDS             complement(9763..10287)
                     /locus_tag="PK144_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNF65485.1"
                     /translation="MKRFHVHLAVEDLAASVRFYTALFGCGPGVLKDDYAKWMLEDPR
                     VNFAISARGRAVGLDHLGFQVGDAEELAAAYAQLAQAGAPVIEQAGTTCCYAKSDKQW
                     VHDPQGIAWETFLTLGEATHYGADLPATATACCAPPAAPAKPVAIAVVAGAPEGGCCT
                     PKAGSGSGSASGCC"
     gene            complement(10382..10891)
                     /locus_tag="PK144_06875"
     CDS             complement(10382..10891)
                     /locus_tag="PK144_06875"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNF65486.1"
                     /translation="MSPTPAAHVHHVLFLCTGNSARSILAEALLNHWGGGRFRAYSAG
                     SFPVGRVNPRALALLEHLGMPTEGLRSKSWDEFAGPEAPALDFVFTVCDAAAGEVCPI
                     WPGHPVTAHWGVADPAAVEGSETEQMAAFREAFRVLENRIKAFTALRPEALDRLALKQ
                     QVDAIGRQG"
     gene            complement(11055..11396)
                     /locus_tag="PK144_06880"
     CDS             complement(11055..11396)
                     /locus_tag="PK144_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF65487.1"
                     /translation="MDQTEAVAALGALAQDSRLAIFRLLVQAGPPGLPAGQIGERLGL
                     APATLSFHLKTLRAAGLVEQRRDGRSLIYAADFARMQALLGYLTEHCCQDDPGVCGLA
                     APTNEDCGCGG"
     gene            11687..11854
                     /locus_tag="PK144_06885"
     CDS             11687..11854
                     /locus_tag="PK144_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65488.1"
                     /translation="MEVQMPRMGAQMARMETPMMRMGAPMTRMEVPMTRMGAPMARRK
                     AQQMRACRAFL"
     gene            complement(11865..>12506)
                     /locus_tag="PK144_06890"
     CDS             complement(11865..>12506)
                     /locus_tag="PK144_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF65489.1"
                     /translation="RMNGTASGDVRLYRDFRGGVRDLACLLLADLSLSTDAWVGEHGR
                     VIDVIRDALHLFAEALAATGDRYALYGFSSRRRDHVRLLALKAFDERHDAGIRGRIAA
                     IRPGYYTRMGAAIRHATTLLGREPASRRILILLTDGKPNDLDRYEGRWGIEDTRAAIR
                     EAREAGLQPFCVTIDEQAHDYLPYLFGTQGWVLIRKPAELPRELPLLYARLTG"
BASE COUNT         2013 a         4488 c         4204 g         1801 t
ORIGIN      
        1 gccgccccgg cgccggccgc gaagccggcg gccacgccgg ccgcgctgcc ggccggtgcc
       61 tggccgttct cgaagcgcat ggaacagcgc gtgccgatga cgcgtctgcg cgcccgcatc
      121 gccgaacgcc tgctggaggc gaagaacacc acggcgatgc tgacgacgtt caacgaaatc
      181 aacatgaagc cggtcatcga cctgcgcaac aagtacaagg accagttcga gaagtcgcac
      241 ggcgtcaagc tcggtttcat gtcgttcttc gtcaaggcgg tggtcgagtc gctgaagcgc
      301 tacccggcgg tcaatgcctc gatcgacggc aacgacatcg tctaccacga ctactacgac
      361 gtcggcatcg cggtcagctc gccgcgcggt ctggtcgtgc cgatcctgcg caacgccgag
      421 gacatgtcgc tcgccgacat cgagaaggcg atcggtgact tcggcaagcg cgcgaaggaa
      481 ggctcgctga ccgtcgagga aatgaccggc ggcaccttca cgatcaccaa cggcggcgtg
      541 ttcggctcgc tgatgtcgac gccgatcctg aaccccccgc agagcggcat cctcggcatg
      601 cacgcgacca aggaccgcgc cgtcgtcgag aacggccagg tcgtcgcacg gccgatgatg
      661 tacgtcgcgc actcctacga tcaccgcatc atcgacggca aggaggcggt cggcttcctc
      721 gtcgccgtca aggaagcgat cgaggatccg gcgcggatgc tgctctccgt ctgagcgcac
      781 gacccggcga tttcggggcg ggggtccggc acccctgccc cgtcgcgcct gcccgttcgt
      841 ttgcgcccca tcccttaccg gaagcactct tttagggaga ctgtcatggc caagacttat
      901 gacgtcgtcg tcatcggcgg cggcccggcc ggctacgtcg cggcaatccg ctgcgcgcag
      961 ctcggcctcg cgaccgcctg cgtcgagaag tggatcaaca tcgaaggcaa gcccgcactc
     1021 ggcggcacct gcctgaacgt cggctgcatc ccgtccaagg cactgctcga atcctccgag
     1081 cactaccaca agctgcagca cggcctcgaa gcccacggca tcagcgtcgg cgaggcgtcg
     1141 gtcgatgtcg cgaagatgat cggccgcaag gagcagatcg tctcgcagct gaccggcggc
     1201 atcgcgcagc tgttcaaggc caacaaggtc gactggctgc agggccgcgg caagctgctc
     1261 gccgagcgcc aggtcgaggt caccggccac gacggcgcgg tcgaggtggt cgaggccgac
     1321 aacgtcatca tcgccgccgg ctcggtgccg atcgacatcg gcgcggcgcc gaaggatgac
     1381 aagctgatcg tcgattcgac cggcgcgctc gccttcaccg aggtgccgaa gcgcttcggc
     1441 gtcatcggtg ccggcgtcat cggcctcgaa ctcggctcgg tctggaagcg cctcggcgcc
     1501 gaggtcgtga tcctcgaagc ggtcgaggac ttcctcgccg ccgccgacca tcagatcgcc
     1561 aaggatgcgc tgcgccagtt ccagaagcag ggcctcgaca tccgcctcgg tgcgcgcgtg
     1621 accggctcga ccaagaccga caccgaggtc acggtccagt acaccgacaa gaacggcaac
     1681 cacgagctca ccttcgacaa gctgctggtc tcggtcggcc ggcgcccggc caccgacggc
     1741 atcgccgatg ccgccgccga tctggtgctg aacgagcgcg gcttcatcca cgtcgatgac
     1801 gagtgccgca ccaacctgcc cggtgtctgg gcgatcggtg actgcgtgcg cggcccgatg
     1861 ctcgcgcaca agggcagcga ggaaggcatc atggtcgccg agttcatcgc cggtaatcac
     1921 ggccacatga actacgacac catcccgtgg gtgatctaca cgcagcccga gatcgcctgg
     1981 gtcggcaaga ccgaacagca gctcaagcac gagggcgtgc cggtcaagat cggcaccttc
     2041 ccgttcgctg ccaacggtcg cgccaaggcc atggatgccg cggccggcat ggtcaagatc
     2101 atcgcccacg ccgaaaccga ccgcgtgctc ggtgcgcaca tctgcgggcc gatggcctcg
     2161 gagctgatcc aggagctggt ggtcgcgatg gaattcggcg cgagcgccga ggacctgcag
     2221 atgaccatcc acggtcatcc gacgctggcc gaagccgtgc atgaggccgc gctgtcggtc
     2281 gacaagcgtg cattgcaccg cgtgaactga gcgcagcccg ccgccacccc ggaccccgcc
     2341 ttctggcggg gtctgtcttt tccggtcctc cccgatgtcc ggtgctcgcg acgatgcgtg
     2401 gcattcgccg tgcatgccgt ccacaatcgg acacacagga gccggcgcag acccgcaccg
     2461 gcaggagatt ccgaccatga gtgccagcac ccggccggtg gcggtcgtca ccggtgccaa
     2521 ccgcggactc ggattcgaaa cctcgcgcca gctcgcccgg cgcggctatc tggtcgtcgt
     2581 caccagccgc catcccgagg ccggcgaggt cgcaagcgaa cgcctgcagg ccgcggccgc
     2641 cgagggcggc ggcgagatcg tgctgcagcc gctcgacgtg acgcgacccg acagcatcgc
     2701 cgcgctcggc gccttcgtgc aggcccgctg cggccggctc gatgcgctgg tcaacaacgc
     2761 cggcgtgctg ctcgacggcc acggcctcga cgatgccacc ggcagcagcg tgttcgccgc
     2821 cagcctcgaa accctgcgca cgaccatgga aaccaacgtc cacggcccgc tgctgctcgc
     2881 gcaggagttc gtgccgctga tgcgccggcg cggctacggc cgcatcgtca atgtatcgag
     2941 cggcatgggc cagctcgctg acatgggcgg ccagtggccg gcctaccggc tgtcgaagac
     3001 ggcgctgaac gcgctgacgc gcatcctcgc cgccgagacc gcccgcaccg gcatcctcgt
     3061 caacgccgtg tgtccgggct gggcgcgcac cgacatgggc ggcccgaagg ccgagcgcac
     3121 gcccgaggag gccgcctccg gcatcgtctg ggccgcgaca ctgcccgacg acggcccgag
     3181 cggcggcttc ttccgcgacg gcaagccgat cccgtggtga gcgcgccgcg cccgcaatct
     3241 cccgcccgcc gccggagtcc cgatcgccgt ggttcccgcc ctgctctgtc tgctcggctg
     3301 tcagctgctc ggtgaggcgc tggtgcagct gacgcgtgca ccggtccccg gtccggtgct
     3361 cggcatgctg ctgctcgccc tctggctcgg tctggtcgcg cgcgggcgca tccccgcggc
     3421 gctcggccag accgccgacg tgctgcttgc gcacctgtcg ctgctgttcg tgccggccgg
     3481 cgtcggcgtg atgctgcatc tgcagcgcat cgccgccgaa tggctggcga tcgtcgcggc
     3541 gctggtgctg agcacgctgc tcgcgatcgc cgcgaccgcc atcgcgatgc gcgcggtgct
     3601 gcgccggctg ccgccgcccg ccgacgacag cgacggcagg gatgcccatg gataggaacc
     3661 tgctcgacat ctgggtctac ctgtcggcgc agccgctgct gtggctggcg gtgacgctcg
     3721 cggcgtatct ggccggctac cggctgttca tggcctcgca ccagcgcgcc tggctgaacc
     3781 cggtggcact ggccatcctg ctgatcgccg ggctgctcgc cgtgaccggc acgccgtatg
     3841 cgcgctactt cgaaggcgcg cagttcgtgc atttcctgct cggcccggcg accgtcgcgc
     3901 tcgccgtgcc gctggtgcgc cactgggcgc gcatccgccg gatgtggctg ccgctcgcgg
     3961 tcgggctcgt cgccggctcg ctgaccgcgg cgctgtcggc gatggcgatc gcctgggggc
     4021 tcggtgccag cctgcgcacg gtgctgtcga tggcgccgaa gtcggtgacg acgccggtgg
     4081 cgatgggcat cgccgagaag atcggcggcc tgccgtcgct gaccgcggtg ctggtgatcc
     4141 tgaccggcat cgtcggtgcc gtgctcggtc cgggcctgct cgcacgcctc ggcatccgcg
     4201 aggacgaagt ccgcggctac gcgctcggcg tcgctgccca cggcatcggc acggcgcggg
     4261 ccttccagct cggcagtctg tgcggcgcct tcgccggact cgcgatcggg ctgaacgcgc
     4321 tgctgacggc gctgctcgtg ccgctgctgg caagcatgct cggcctgtcc tgaacggcca
     4381 cggctgctag attcctcgca ggccgcgccc ggccctgtcc acgactgcgc gcccgctgcc
     4441 acccgcagca gcggcgccga ttgcaatgcg tccgttccct gcacacctcc ttccacccgc
     4501 acgcctgttg ccctgcctgt cattcggagg ccccgatgcc acggatttcc gcccgcctgc
     4561 ccgctgttgc cgcgctggca ctgctggccg cctgcaccgt cacgcccggc gtgatctccg
     4621 gtccgcgcgg cccggccgcg ctgccgcccg acaccgtctg gctcgatcag aactggtccg
     4681 acgaggaccg cgacctctgg tggttcaccg gccagggctc gcacatgatc ccgtacgact
     4741 ggttcctcgc cctgcccggg gccgacggca agccgttccg ggccgatccg accctcgccg
     4801 gctacggctt cctgaccacg ccggcgaccg tgcgcaatcc cgacgcgctg ccggtcggct
     4861 tcgtcaagga tcagcgcccg gccaccgaca tccgcggcca gtggctcggc atgacctgcg
     4921 cggcctgcca caccgcgcag ctgcgcatca aaggcgtcga ggtgcgcgtc gacggcggcc
     4981 ccgggctggt cgatttcgac ggcttcatga acggcatggt cgcggcgatg gaccgcgccg
     5041 ctgccagccc cgacgccttc tcgcgcttcg ccgccgaggt gctcggcagc aacgccacgc
     5101 ccgaaaaact cgccgacctg cgcacggcct tcggcgagca cacgcagttc ctgaagacgc
     5161 gcatcgccgc caacgccatc ccgcgcccgg ccggccacgg ccgcgtcgat gccttcggcc
     5221 agatcttcaa ccaggcggtc gtgcacgcgc tcggcgtgcc cggcaacgcc cacccggccg
     5281 atgcaccggt ctcgtacccg ttcatctggg atgcgccgca gcatgatcgc ctgcagtgga
     5341 acggcagcgc actgaacctc ggggccgggc cgctggcgcg caactccggc gaggtggtcg
     5401 gcgtgttcgg caccgtgaac gtgaaccagc ccggcttcgg catgcgcggc tatgcgtcct
     5461 cgctcgacac gcagcagctc gacggcctcg aacgcctgct gacgacgctg tggtcgcccg
     5521 aatggccgtc gaagtacgcc ggcccgctcg atgcgacgct ggtcgcgcgc ggcaagacgc
     5581 tgttcgccgc caactgcgca agctgccacc agaacatcga cgcgaagggc agcgtcacct
     5641 tcgatcgtgc cgatccgaac cgccgcatca ccgcgcagat gatcgacctc ggcaaggtcg
     5701 gcaccgatcc gcacatggcg aagaacgtgc tcgcctgcac cgccgacacc ggcccgctga
     5761 acggccggcc ggtcgcggtg ctgccgttcg ggccgaagct caagccgaag gatctgtgcg
     5821 tgaacgtcac cggcaacgtc gtcgtcggcg cgattctcgg caacaacccg ccggtcagcc
     5881 tgttccaggt gctggagttc ctgaaaggca gccaggccct gaaggccaag ctgatgaacc
     5941 cgcagtacaa ggcccggccg ctgaacggca tctgggcgac ggcgccctac ctgcacaacg
     6001 gctcggtgcc gacgctcgcc gacctgctgc agccgccggc gcagcgtccg aagaccttcc
     6061 acgtcggcga tcgcgaattc gacgccggaa aggtcggcta cgtcaccgac cgcggtccgt
     6121 tcgtctacga caccgccgag ccgggcaaca gcaatgccgg ccacctgtac ggcacgaagc
     6181 tcgccacggc cgacaagcgc gcgctgctgg agtacctgaa gtcgctgtaa gcgccgtttc
     6241 tgcacccgcc agccccgccg acggcggagc ctcccggaag cggaggctcc gccgtcggtt
     6301 tttgcgccgc gtgccgggct cgcggcgact acgctcgcgc ttcaacaccg gcgggagaac
     6361 gtgcagcgat gactcatctg atcggcagcg actggcagcc cggcggcggc gaatccttcg
     6421 cgtcggaaaa ccccgcgacc ggcgcgatgc tctggcgcgg ccatgccgcc gatgccgccg
     6481 atgtcgatgc cgcggtgcgc gccgcacgca cggccttccc ggcctgggca gccctggact
     6541 tcgaggcgcg cgtcgaacgg ctgcgcgcct tcgaggccac gctgcaggcc aacgccccgg
     6601 aactcgccgg caccatctcg gccgagaccg gcaagccgcg ctgggaaacg gcgaccgagg
     6661 tcgcggcgat gatcgccaag atcggcatct cgatcgccgc gcacgccgag cgcaccggca
     6721 cgcgcgaagg cacggccgcc ggcgtgcgcg ccgtgctgcg ccaccggccg cacggcgtgg
     6781 tcgcggtgtt cggcccgtac aacttccccg gccacctgcc gaacggccac atcgtgccgg
     6841 cgctgctcgc cggcaatacc gtcgtgttca agccgagcga gcagacaccg ctggtcgccg
     6901 agcgcaccgt gcagctctgg caggcttcgg gcctgccggc cggcgtgctg aacctcgtgc
     6961 acggcgcgcg tgcgaccggg caggcgctgg ccacgcatcc ggaactcgac ggcctgttct
     7021 tcaccggcag ccacgccacc ggcgaatggc tgcaccgcag ctttgccggg cgcaccggcc
     7081 gcatcctcgc gctcgaactc ggcggcaaca atccgctgat cgccgatgcc gtcacgctct
     7141 ccgacctcga cgccgcctgc tggcacatcg tgcagtcggc ctggatcacc gccggccagc
     7201 gctgcacctg cgcgcgccgg ctgatcgtgc cgctcggggc acgcggcgat gcgctgatcg
     7261 aacggctctg ccagtggctc gacggcatcc gcatcggcgc gcccgatgac gaacccgagc
     7321 cgttcatggg gccgctgatc tcgaacgccg ccgccgatgc gctgctgaac ttccagaccg
     7381 atctcgaact ggccggcgcc cgggtgctgc gccgcgccca gcgcctcgac cccgcccgtc
     7441 cgctgctgac gccggcgctg ctcgacgtga gcgcctgcag cctgcgcccc gacgccgaag
     7501 ccttcggccc gctgctgcag ctcgtgcgcg tcgccgactt cgagaccgcg ctctgggagg
     7561 ccaaccacac gcgcttcggc ctcgccgccg ggctgctgtc cgacgatgcg acgcgctggc
     7621 agcagttccg caccacgatc cgcgccggca tcgtcaactg gaaccgcccg accaccggtg
     7681 cggccagcag cgccccgttc ggcggcatcg gcgacagcgg caacctgcgc ccgagcgcgt
     7741 attacgcggc cgactactgc gcgtatccgg tcgcgtcgct ggaggcggat acgctcacgc
     7801 ggccggcgca ggcagggacg gggctggtgc cgccggcatg agacaaccgc agcgcccgcc
     7861 cgggccaccg cgacgggatg gacgccagaa gtccggtgcg tcaggggggt ggtaattctc
     7921 gctgcgggat gcaggtcggg gtcggaatgc gcctgcgcag tgcaggcagt cgatggcgcg
     7981 taccggaatc ccgacgctat agttcatcgg cgcacccgcc tgcgtgtttc cggatacttt
     8041 tgcagacgct ggtttgctgg catgacgcgg aaggcggatt acggtgtgcg gaaaggatgg
     8101 tgagccttga tacgccacgt aatgtgcacc tgagccgccc tgcgtgccca atacatcgaa
     8161 ggaactccaa tgcctatcgt aatcgcttca tccttcgctg accctgccga catcgcggca
     8221 tacaagaagg ctattgctgc cggtaaaacc gaggcagagg cgcttaaagt tggcgataac
     8281 ggaatcggca aatggggaga tgacacaacg cgagaagacg tcccgatatg tgcgctgcct
     8341 ccagaggatt gggaaggcaa gtggggcaag ggcgataccg ccagaggcaa aaaggttgcc
     8401 gtaacataca aaggcaagac ggttatagga gaacttaggg ataccatgcc ccgcaaagca
     8461 aacatcaaga acggggctgg tattgatctg aacccaggct tcgcgaaggc attcggagtc
     8521 agacaaccat ttatgcttaa agacgtcacc tgggagtggg cctgacccat ctcattatac
     8581 caaccacagg gccgtaggct gggttgagga acgaaaccca gcatcggaag ctaccccgtg
     8641 tgatgactgc cgggtttcct tcgtcaaccc agcctacggg ctctgatacg cagccctcac
     8701 gccaccgccg ccacaaacca ccgccgctgc agccagccgg cgacgtgcac caagccgatc
     8761 agcgccggca cctcgaccag cgggccgatg acggctgcga aggcttcgcc ggagttcagg
     8821 ccgaacaccg cgacggcgac cgcgatcgcc agttcgaagt tgttgctcgc ggccgtgaac
     8881 gacagcgttg cggtctggcc gtagggcgcg ccgtagcgtc gtccgagcgc gaagctcagc
     8941 aggaacatcg cgacgaaata gatcgccagc ggcacggcga tgcgcagtac gtcgagcggc
     9001 aggcgcacga tcagttcgcc cttcagcgaa aacatcacga ggatcgtgaa cagcagcgcg
     9061 atcagcgtca gcggcgagat gcgcggcacg aacaccgtcc ggtaccagtc atcgcccttc
     9121 atccggcgca acaccagccg cgtcacgaaa ccggcgagaa acggaatgcc gagatacacg
     9181 aacaccgtcc acgccacctc gccgatcgag acttcgacct tgaaggtttc aagtccgagc
     9241 cagcccggca gcacgctcag gaacagccat gcatacaccg ggaagaacag cacctggaac
     9301 agcgcgttga aggccaccag cccggcgcag tactcggtgt cgccacgcgc gagctcattc
     9361 cagacgatga ccatcgcgat gcagcgcgcc agcccgatca ggatcagccc ggccatgtaa
     9421 tgcggctgat ccggcagcag cagcacggcg agcgcgaaca tcagcagcgg cccgatcacc
     9481 cagttctgca gcagcgacag cagcagcacg cgcgtgttgc ggaacacctg cggcagcgcc
     9541 tcgtagcgca cgcgcgcgag cggcggatac atcatgacga tcagcccgat cgcgatcggc
     9601 caactggtcg tgccgctcga caggccctgc caaaagccgg caatgccggg gaagaaatgc
     9661 ccccaggcca cgccgatcgc catcgccgcg aagatccaca gcgtcagcca gcgatcgagg
     9721 aacgacaggc ggcgggagac ggaagccatg cgcacgcccc cgtcagcagc agccggaagc
     9781 ggagccggag ccgctgccgg ccttcggcgt gcagcagccg ccttccggcg ccccggccac
     9841 gaccgcgatc gcaaccggct tcgccggggc tgccggcggt gcgcagcagg cagtcgcggt
     9901 cgccggcagg tcggcgccgt agtgcgtcgc ctcgccgagc gtcaggaagg tctcccaggc
     9961 gatgccctgc ggatcgtgca cccactgctt gtcggacttc gcatagcagc aggtcgtgcc
    10021 ggcctgctcg atgaccggcg ccccggcctg cgcgagctgc gcgtacgccg cggcaagctc
    10081 ttcggcatcg ccgacctgga agccgagatg atcgagcccg accgcccggc cgcgggcgct
    10141 gatcgcgaag ttcacgcgcg ggtcctccag catccacttc gcgtaatcgt ccttgagcac
    10201 gcccggcccg cagccgaaca gcgcggtgta gaagcgcacc gaggcggcga gatcctcgac
    10261 ggcgagatgg acatggaagc gtttcatggc ggtggctccg ggggggggga aggaatcggt
    10321 ggacggactt tgcagcccga tgaacgccgg ggctctgcgc gccctggccg cgggatcggt
    10381 atcagccctg gcgcccgatc gcatcgacct gctgcttcag tgccagccgg tcgagggcct
    10441 cggggcgcag ggccgtgaag gccttgatgc ggttttcgag cacccggaac gcctcgcgga
    10501 aggccgccat ctgttcggtc tcgctgccct cgaccgccgc cgggtcggcc acgccccagt
    10561 gcgcggtgac cggatgcccc ggccagatcg ggcagacttc gccggccgcc gcatcgcaga
    10621 ccgtgaacac gaaatcgagc gccggcgcct ccggcccggc gaactcgtcc caggacttcg
    10681 agcgcagccc ttcggtcggc atccccagat gttcgagcag cgccagcgcg cgcggattga
    10741 cgcgcccgac cgggaagctg ccggcgctgt acgcccggaa gcgtcccccg ccccagtgat
    10801 tgagcagcgc ctcggcgagg atgctgcggg ccgagttgcc ggtgcagagg aacagcacgt
    10861 gatggacgtg agcggcgggc gtggggctca tgggcaggct ccgggggtgg atgcagcgat
    10921 tatatttcca tgtttatgga aatatcaaaa tcctcagcct gccatgctgc aacttccgtg
    10981 ttgcggaaaa ccggtcgccg cctgccggcg aggcaagccg cagcgacccg cgcgcgtact
    11041 gcggctttcc cccgtcatcc gccgcagccg cagtcctcat tcgtcggcgc ggccagcccg
    11101 cagacgcccg ggtcgtcctg gcagcagtgt tcggtcaggt agccgagcag ggcctgcatg
    11161 cgcgcgaagt cggcggcata gatcagcgag cggccgtcgc ggcgctgctc gaccagcccg
    11221 gcggcgcgca gcgtcttcag gtgaaacgac agtgttgccg gcgccagtcc gagccgttca
    11281 ccgatctgtc cggccggcaa tccgggcgga ccggcctgca ccagcaggcg gaagatggcg
    11341 aggcggctgt cctgcgcgag ggcgccgagc gcggcaacgg cttcggtctg gtccacggcg
    11401 gggttccggt gaggggggat ggagcactgt agcgcgtgcg cgcgacgcca cgagccggtc
    11461 gtcggctcgc ccccggagcc cgctcaggct tcctgagcgc gcgtgcaagc gggtgtgcgg
    11521 cctcgcaccg aggctgccga tgcaggcgga gcagccggca cggaccgcgc aggcgccccg
    11581 gcacgcaccg atcccgatgg tcgtccggat gccgatccgg cgcggatccc cccgtccgac
    11641 cggccgccac cgccgaagcc gtcccggcgg cttgcggatg acgcgcatgg aagtgcagat
    11701 gccgcgcatg ggtgcgcaga tggcgcgcat ggaaacgccg atgatgcgca tgggcgcgcc
    11761 gatgacgcgg atggaagtgc cgatgacgcg catgggtgcg ccgatggcgc gcagaaaagc
    11821 gcagcagatg cgtgcctgcc gggctttcct gtgaaccgcc ccgctcaccc cgtcagccgc
    11881 gcatacagca gcggcagttc gcgcggcagt tcggcgggct tgcggatcag cacccagccc
    11941 tgcgtgccga acaggtacgg caggtagtcg tgggcctgct cgtcgatggt cacgcagaac
    12001 ggctgcaagc cggcctcgcg cgcctcgcgg atcgccgcgc gcgtgtcctc gatgccccag
    12061 cggccttcgt agcggtcgag atcgttcggc ttgccgtcgg tgagcaggat caggatgcgc
    12121 cggctcgccg gttcgcggcc gagcagcgtc gtcgcgtggc ggatcgccgc gcccatgcgc
    12181 gtgtagtagc cggggcggat cgcggcgatg cgcccgcgga tgccggcgtc gtggcgctcg
    12241 tcgaaagcct tcagcgccag caggcgcacg tgatcgcgcc ggcgcgacga gaagccgtag
    12301 agcgcgtagc ggtcgccggt cgcggccagc gcctcggcga acaggtgcag cgcgtcgcgg
    12361 atcacgtcga tgacgcgacc gtgctcgccg acccaggcat cggtcgacag cgacaggtcg
    12421 gcgagcagca ggcaggcgag atcgcgcacg ccgccgcgaa agtcgcggta caagcgcacg
    12481 tcgccgctcg ctgtaccgtt catgcg
//
LOCUS       DAOQIE010000081        12214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2542_length_12214_cov_5.519368, whole genome shotgun sequence.
ACCESSION   DAOQIE010000081 DAOQIE010000000
VERSION     DAOQIE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_12214_cov_5.519368"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PK144_06895"
     CDS             <1..1026
                     /locus_tag="PK144_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNF65490.1"
                     /translation="AGWERWSPVERFAGATREAPVYALDAASGRIRFGDGIRGRVPAP
                     GRRIRAVEYRHGGGAAGNLGPGQLRSVVEAPMLRVRHERALRGGADAETVAEAERRLP
                     DWLNHRERAVTAADFRMLALETPGSGIARAEVVRGLIPGDTLAALQRNVPGAVAVFVL
                     AARAPDDPAPPRPGAAQLREVWAWLRERCPIGTELYVLSPRFVPLAVSVAVGVRDPAI
                     ERETLLRVEAALRQWLSALHGGADGRGWPLGRAVDPEELRAVAARVDGVVRVPALGLF
                     APDAAGRWLPLPAGTALALLDVDLPELRAVSAGSGNSVQLPAALTDAAEAASDAPAPA
                     PVVPDLC"
     gene            1040..3388
                     /locus_tag="PK144_06900"
     CDS             1040..3388
                     /locus_tag="PK144_06900"
                     /inference="COORDINATES: protein motif:HMM:NF021220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNF65491.1"
                     /translation="MDVNGSSALLLASAAAFAGEAEGVQWDAAGNCLRLADAGRPRWP
                     QRDRAAGLALRAASPLRLLDGFGRLGAADGDRLEAIAADGSRSAIPDDAGQPIAIAAS
                     ARWTALGLGGDDRLAAAWTDPAAADAAARDGLDVLHLRRRWHVRLAPGFAVAALHVDG
                     SGRVWLAGAGEYALAAGAPLPQPFVPGPGFRPLAEDPDPLTLRWRAPLPPGEVLALAG
                     DATALYLLLDRGDGAQDLARRALGDEPEPWQVFPIPADAALPWASDLAAPGDGTLLLA
                     CAPQPDDAGFRACDVPQVRLIEDDDGGAHVEALRLRWPQMARPQSARVVISADGRPRY
                     LAADGPRPWLKLPQPEYAPAGQLDTAPAALDGRAPGTVWHRLYLEACLPPGCRLRLAA
                     RAADTPAALAAADWQPQPAPVWNALPSELPWHPGLLAPQRPGERGLFELLLQQADGNV
                     RRLAGRWLQLRITLAGDGRRTPQLHALRVYAPRFDYQERYLPELFRQQESRREAPPAP
                     ANGADLRERLLALCEGLLTPLEGRIAAAETLLDPQAAPAALLPWLAGYLGRTPLADWP
                     EARVRRWLAHLGELLAWRGTLRGVVLALDLATDGAVARGEIVPVENFRLRRSMATLLG
                     LLPDADAHPLTLGTTRSGNSIVGDSLILADSLDLDRPETRAFLALFAPEQARAGDERA
                     AVRVFFERYAHRLSVLLHGPGRAWQGVVEQVLEDELPAHLARDVYPAEFPFVPGLSPL
                     LGYDTWLETTPPPAGVQLDFTRLGRAGVLLDAASLLPAGAPS"
     gene            3462..4280
                     /locus_tag="PK144_06905"
     CDS             3462..4280
                     /locus_tag="PK144_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65492.1"
                     /translation="MTDADQDSPLRHDAEAGPLARVAFRPGMLLGVEALLAEQDYHRR
                     RLNRHARLLHGFGTIYGLLVTLEPPAAPLPLRLVVHPGIALDALGRELLLPEPYGIDL
                     PAWLAEQGAEALLPFSTPPDAAEGAPRSLVLEVALRYVDCALGLQPVLAAATNASTDA
                     VAASRAGEAFELLLRGADLNPPPPDPPPATLPSPAEQARLDAAADEAERARLQGRLDL
                     LHRTRGATTLPPLGPGDASLVLARVTLTLTTLSAPQPAAADVAVDNLIREFAAS"
     gene            4294..6051
                     /locus_tag="PK144_06910"
     CDS             4294..6051
                     /locus_tag="PK144_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65493.1"
                     /translation="MTTVFQPVVPAAPEGPQAPLRATPIDARLSRTYYFDGRLLTAAD
                     LIRDQQYLDRRLLDVGRGLGDGIVEGLALALSGSTLSVQPGVAVSADGRTLQLDAPLL
                     VDLDDRATLAALNTGADSDGVLHSGRFTNGLYAVALGYAEQGSGSAEVFPRDFGAARG
                     LNFDAIDEGVELRLVPLTVPAPPLPALAARALLARTLVGAGRLDHWLPADAVALGVLA
                     IDGDAVLWCDPWLLRHPRRPDAVLDGFQEDLLAHYRALLAEVLAERAGASFHAGEHFH
                     VLPPAGPLPRACVDPVRGQQTFFPAECRVQLVPLRADDMEAVLQSALALAPLDLDAPQ
                     PAEVLVLVALDAGPYANLAEALLARAQSDALGGEDVLAPVVTLSGETDPASPWFPFWE
                     AVPEGRVYYLRRPARAAADGLAGIVLASGYTVPEALPPPAPEPELPPPDTGEPPPDTT
                     VPPPEIVTVAVLPEWLRSDALARLRPAPEAATREQRSVLALLDEEQASREGTAAGYPL
                     TGSVSAALLHIGPMTDSALWPTLAGLAGSGGSRAIDTWLETFRRAEADGADVFRAGVA
                     AGRELGLDTTLLRLWSSIG"
     gene            6136..9477
                     /locus_tag="PK144_06915"
     CDS             6136..9477
                     /locus_tag="PK144_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65494.1"
                     /translation="MADFFRLSPPQPTPSGQSRPHLFPLRSLGAADFDRLDRYRDTRL
                     AALLAGRPAGIVEGLRIGAQPGGDSVVVRPGLGIAGDGRTLALRTPLRVAWSELIGPA
                     QGDGVYLICLAQRLRRQARDVSVPCRRGELDPLRDLRADTESFLTLRGPLGGYAPEPI
                     DGDAVLIANRLGTRFLDAFPRATIGDGLALGLVAVQAGAPLWVDVEAARFAAVPEAAR
                     RALLEHTLAVIARGRWASLETAPAGAPLPRAWLSLPAPDGTLPLCANLPAHLHLRLLP
                     VRASEFAPALAAAQARGLLQLRDGRADHVHLLLAIPDADYRPDLLSIPAPDTPTLDAL
                     YRAWLSAQRAWAAAREVREQLWRVTDEDRTSLGDDVLAALLPDPDPVEPAAPAEPAAL
                     LAALAQRHAGETLTAPPGYASFGAWSEAHANDGGPAAVGFTGSGARIRLARAATRLVQ
                     LQRSLAARTDRLDGIRDFLGRLRQQLDAHTGSLAALSGGVASDGSGLRIARWLPYVSL
                     TAPATTIAAAATPIADARTLSFELPLANAAPIAEIAVRPSFTTTLFETQGVQAAGVQQ
                     RFDRVLASGGGLNQQIRDTLAQIPRAAEPATPAAFQAQSADYGVIRHVLPEVYALQDA
                     GSNLAGLRDEINERLFPDRPPPNSDRPSPRLDATAIRRLYLDVPATQRYPRLIELGRH
                     LHADTTLLERLRTRMETAIRRRLQEIEQQKALIARLQREIAGLEAGLAARRPELDDTA
                     RSYAMAQALTADDWARVAGARRQRRRVLASARGFGYVRALPWTITQRPAPAVALAAVE
                     ADELVPGLTQAAAETLPEALAEFAEALFEVPVGEWRSLRGLLVELPQRRVLDEAIVLR
                     AQRLGLRSQRTTSSGPRYAGLERASRALYAQAAVLVPVDAAVSLQRQREQAAHAYSLE
                     DALHGPPGRLRRAAEALRAKLEPALRAAAYWSRRLPAPLLLELADRADEGTLDTAHPE
                     RWPAPPAGSSETVLTDWQRWQALLRWLNAQLPEPAPTGRAALDHLLRALLLAAAHGDL
                     ALATGGRVAAGATLRPGQTLRLTLERELRPGTRLELRDAQQQLVGRLQLDDIDAQGLA
                     SARIVEALTPGVAVTTAFRVSTSTS"
     gene            9474..11168
                     /locus_tag="PK144_06920"
     CDS             9474..11168
                     /locus_tag="PK144_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65495.1"
                     /translation="MNPAAAAARSTTIGRLRLRSDPAHAPALRTALELADWPQPEGDA
                     IVLVRRIVVRGAAASTLAAAAGVRLAAELRAAVAGDAPSAADAGAVRFTGPAALYAQL
                     SRDLAAGRAARCWYWARWRRLFALPVTSAIPELWREQPLRLAAISAELARSGDLARLW
                     RALPPHEVQRLVQTLAAASGLPPPAEPAAGRDDDPASASPPPVPPPALLSRWQPACRG
                     LPAGDARVRLALYLLLLDWRPALAADATACRQLAQRLCGIVDPERPAPHRSLLPEQTH
                     SPALPHPVAVAVTASAAHTEPAKAAAIHPASTDGQEASDSRTTAVAKDVFVTTPDSRL
                     APVSRERPSIAVPETCPAPAPMPEPANRTSRTCLPDPSPGIAASHDAGRSGALREPPA
                     STAALSCSVHADTGVDDWIDTGSGGLPYLLNVLNHRRVQTLLGGLHGLDARGGGWHAW
                     LVLGRALGLAPDDGVGRWLERRWQLHVGRDEAWQPQPGDPALVALAAALCGPQLWSPA
                     LIAGPGRLRADSTHLDFERPLAGVQLAVRRAGLDLDPGWLPWLGRVVAIRYVERWP"
     gene            11165..>12214
                     /locus_tag="PK144_06925"
     CDS             11165..>12214
                     /locus_tag="PK144_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF65496.1"
                     /translation="MNAPAVSGLPGESSARLSAWVRTGLTYFATQCYDALPAPVTLLD
                     ASPGDWPQLCTAVPEAVATAADALARLARAYRLRPAELFLIALAGEIEADFRVVLAIA
                     ELQAPERTARPALHLACALTETLFPQQKRLTPLTLAGCTAVRAGLLAIDGDGPLPMRS
                     LRLDARLWATLGGDDTVWPDTQWLTPADAELPAATRARLDPLARALADGDFDVLVLRG
                     MPYSGRGRCAAALAARLGLQACAITSERWAADAALAAACHCCGWLPVLRPRLAPGERY
                     EPPRHPGYDGALIVLLGEAGAVAGTRVFEETLPAATAGERAASWAAAGIPDPPATVLG
                     GPAIAALLRSAAWQAG"
BASE COUNT         1379 a         4837 c         4434 g         1564 t
ORIGIN      
        1 gccggctggg agcgctggtc gccggtcgag cgcttcgccg gcgccacccg cgaggcgccc
       61 gtctacgcac tcgatgccgc gagcggccgc atccgcttcg gcgacggcat ccgcggccgc
      121 gtgccggcac cgggccggcg catccgcgcc gtcgaatacc gccacggtgg cggcgcggcc
      181 ggcaatctgg ggccggggca gctgcgcagc gtcgtcgagg cgccgatgct gcgcgtgcgc
      241 cacgaacgcg cgctgcgcgg cggtgccgac gccgagaccg tcgccgaggc cgaacgccgg
      301 ctgccggact ggctgaacca ccgcgaacgc gcggtgaccg ccgccgactt ccggatgctc
      361 gcgctcgaaa ccccgggcag cggcatcgcc cgcgccgagg tcgtgcgcgg gctgatcccc
      421 ggcgacacgc tggccgccct gcagcgcaac gtccccggtg cagtcgcggt gttcgtgctg
      481 gcggcgcgcg cccccgacga cccggccccg ccgcggccgg gcgccgcgca gctgcgcgag
      541 gtctgggcct ggctgcgcga acgctgcccg atcggcaccg aactctacgt gctgtcgccg
      601 cgcttcgtgc cgctcgcggt ctcggtcgcg gtcggcgtgc gcgacccggc catcgagcgc
      661 gagacgctgc tgcgcgtcga agcggcgctg cgccagtggc tgagcgcact gcacggcggt
      721 gccgacggcc gcggctggcc gctcgggcgc gccgtcgatc ccgaggagct gcgcgccgtc
      781 gccgcgcgcg tcgacggcgt cgtgcgcgtg ccggcgctcg gacttttcgc ccccgatgcc
      841 gccggtcgct ggctgccgct gccggccggc acggcgctcg cgctgctcga tgtcgatctg
      901 cccgagctgc gcgcggtctc ggccggcagc ggcaacagcg tgcagttgcc ggccgccctc
      961 accgatgccg ccgaagccgc cagcgacgcc ccggcgccgg cgccggtcgt gccggatctc
     1021 tgctgatgga cgtgagctga tggacgtcaa cggcagttcc gcgctgctgc tcgcctcggc
     1081 cgcggccttc gccggcgagg ccgagggcgt gcagtgggat gccgccggca actgcctgcg
     1141 gctcgccgat gccggccggc cgcgctggcc gcagcgcgat cgcgccgccg ggctggcgct
     1201 gcgcgccgcg agtccgctgc gcttgctcga cggcttcggc cggctcggtg cggccgatgg
     1261 tgaccggctc gaagcgatcg ccgccgacgg cagccgcagc gcgatccccg acgacgccgg
     1321 ccagccgatc gcgatcgccg ccagcgcccg ctggaccgcg ctcggactcg gcggcgacga
     1381 ccggctggct gcggcatgga ccgacccggc cgctgccgat gccgccgcgc gcgacgggct
     1441 tgacgtgctg cacctgcgcc ggcgctggca tgtgcgcctc gcgcccggct tcgcggtcgc
     1501 ggcgctgcac gtcgacggca gcgggcgcgt gtggctcgcc ggggccggcg aatacgcgct
     1561 cgccgccggt gcgccgctgc cgcagccctt cgtgccgggt ccgggcttcc ggccgctcgc
     1621 cgaagacccc gatccgctca cgctgcgctg gcgcgcgccg ctgccgcccg gcgaggtgct
     1681 cgcgctcgcc ggcgatgcga cggcactgta cctgctgctc gatcgcggcg acggcgcgca
     1741 ggatctcgcc cgtcgtgcgc tcggcgacga acccgagccc tggcaggtgt tcccgattcc
     1801 ggccgatgcc gcgctgccgt gggcgagcga tctcgccgcg cccggcgacg gcacgctgct
     1861 gctcgcctgc gcgccgcagc ccgacgatgc cggcttccgc gcctgcgacg tgccgcaggt
     1921 acggctgatc gaagacgacg acggcggtgc gcacgtcgag gcgctgcggc tgcgctggcc
     1981 gcagatggca cggccgcagt cggcgcgcgt cgtgatttcg gccgatggcc ggccgcgcta
     2041 tctcgccgcc gacggcccgc ggccgtggct gaagctgccg cagcccgaat acgcaccggc
     2101 cggccagctc gacaccgcgc ccgcggccct cgatggccgc gcccctggca cggtctggca
     2161 tcggctgtac ctcgaagcct gcctgccgcc cggctgccgg ctgcggctcg ccgcacgcgc
     2221 cgccgacacg ccggccgcac tcgctgccgc cgactggcag ccgcagccgg cgccggtctg
     2281 gaatgcgctg ccctcggagc tgccctggca tcccggtctg ctcgcgccgc agcggcccgg
     2341 cgagcgcggg ctgttcgagc tgctgctgca gcaggccgac ggcaacgtcc gccgcctcgc
     2401 cgggcgctgg ctgcagttgc ggatcacgct cgccggcgac ggccggcgca cgccgcagct
     2461 gcacgcgctg cgcgtgtacg cgccgcgctt cgattatcag gagcgctacc tgccggagct
     2521 gttccgtcag caggaaagcc gccgcgaggc accgccggcc ccggccaacg gcgccgacct
     2581 gcgcgaacgc ctgctcgcgc tctgcgaggg tctgctgacg ccgctcgaag gccgcatcgc
     2641 cgccgccgag acgctgctcg atccgcaggc ggcgccggcc gcgctgctgc cctggctcgc
     2701 gggctatctc gggcgcacgc cgctcgccga ctggcccgag gcgcgcgtgc gccgctggct
     2761 cgcgcatctg ggtgagctgc tcgcgtggcg cggcacgctg cgcggcgtcg tgctggcgct
     2821 cgatctcgcg accgatggcg ccgtggcccg cggcgagatc gtgccggtcg agaacttccg
     2881 gctgcgccgc agcatggcga cgctgctcgg cctgctgccc gatgccgacg cgcatccgct
     2941 gacgctcggc acgacgcgct cgggcaactc gatcgtcggt gacagcctga tcctcgccga
     3001 cagcctcgac ctcgaccggc ccgaaacccg cgcgtttctg gcgctgttcg cgccggaaca
     3061 ggcacgggcc ggcgacgagc gcgccgcggt gcgggtgttc ttcgaacgct acgcgcaccg
     3121 cctgagcgtg ctgctgcacg gccccggccg cgcctggcag ggcgtggtcg agcaggtgct
     3181 cgaagacgaa ctgccggcgc atctggcccg ggacgtatac ccggccgaat tcccgttcgt
     3241 gcccggcctg tcgccgctgc tcggctacga cacctggctg gaaaccacgc cgccgccggc
     3301 cggcgtgcag ctcgacttca cccgcctcgg ccgcgccggc gtgctgctcg atgccgcgtc
     3361 gctgctgccc gctggagcgc cgtcatgacc gggaccgcta ccgatttcga ctctccgctg
     3421 cgccacgacg cagtggcaat gctgcccgct ggagcgcggc catgaccgat gccgatcagg
     3481 actctccgct gcgccatgac gccgaagccg gaccgctcgc gcgcgtcgcg ttccgccccg
     3541 gcatgctgct cggcgtcgag gcgctgctcg ccgagcagga ctatcaccgc cggcggctga
     3601 accgccatgc gcggctgctg cacggcttcg gcacgatcta cggcctgctg gtgacgctgg
     3661 agccgccggc cgcaccgctg ccgctgcggc tggtcgtgca tccgggcatc gcgctcgatg
     3721 cgctcgggcg cgaactgctg ctgcccgagc cctacggcat cgacctgccc gcctggctcg
     3781 ccgagcaggg cgccgaggcc ctgctgccgt tcagcacgcc gcccgatgcc gccgaaggcg
     3841 cgccgcgctc gctcgtgctc gaagtcgcgc tgcgctacgt cgactgcgcg ctcggcctgc
     3901 agccggtgct ggccgccgcg accaacgcct cgaccgacgc agtcgctgcg agccgcgccg
     3961 gcgaggcctt cgagctgctg ctgcgcggtg ccgacctgaa cccgccgccg cccgacccgc
     4021 cgccggcgac gctgccgagc cccgccgagc aggcccggct cgacgccgcc gccgacgagg
     4081 ccgaacgcgc gcggctgcag ggtcggctcg atctgctgca ccgcacgcgc ggcgcgacga
     4141 cgctgccgcc gctcggcccc ggggacgcga gcctcgtgct cgcgcgcgtg acgctgacgc
     4201 tgacgacgct gtccgcgccg cagccggccg cggccgacgt ggccgtcgac aacctgatcc
     4261 gcgagttcgc ggcttcgtga cggaggcttc gcgatgacca ccgtgttcca gccggtcgtg
     4321 ccggccgcgc ccgagggccc gcaggcgccg ctgcgcgcca cgccgatcga tgcgcgcctg
     4381 agccgcacct attacttcga cggccggctg ctgaccgctg ccgacctgat ccgcgaccag
     4441 cagtacctcg accgccgcct gctcgatgtc gggcgcgggc tcggcgacgg catcgtcgag
     4501 gggctcgcgc tcgcgctgtc cggcagcacg ctgagcgtgc agcccggcgt cgcggtcagc
     4561 gccgacggcc gcacgctgca gctcgatgca ccgctgctcg tcgatctcga cgaccgcgcc
     4621 acgctcgcgg ccctgaacac cggcgcagac agcgatggcg tgctgcactc cgggcgtttc
     4681 acgaacggcc tgtacgcggt cgcgctcggc tacgccgaac agggcagcgg cagcgccgag
     4741 gtgttcccgc gcgacttcgg cgccgcgcgc ggcctgaact tcgacgcgat cgacgaaggc
     4801 gtggaactgc gcctggtgcc gctgaccgtg ccggccccgc cgctgccggc gctcgcggcg
     4861 cgcgcgctgc tcgcgcgcac gctggtcggc gccggccggc tcgatcactg gctgcccgcg
     4921 gatgccgtcg cgctcggcgt gctcgcgatc gacggcgatg ccgtgctctg gtgcgacccc
     4981 tggctgctgc gccacccgcg ccggcccgat gccgtgctcg acggctttca ggaagacctg
     5041 ctcgcgcact accgcgcgct gctcgccgag gtgctcgccg agcgcgccgg cgcgagcttc
     5101 cacgccggcg agcacttcca cgtgctgccg ccggccggtc cgctgccgcg tgcctgcgtc
     5161 gatccggtgc gcggccagca gaccttcttc ccggccgaat gccgcgtgca gctcgtgccg
     5221 ctgcgcgccg acgacatgga agccgtgctg cagtcggcgc tggcgctggc cccgctcgat
     5281 ctcgacgcgc cgcagccggc cgaggtgctg gtgctggtgg cgctggacgc cggcccgtac
     5341 gcgaacctcg ccgaagcgct gctcgcacgc gcgcagagcg acgcgctcgg cggcgaggac
     5401 gtgctggcgc cggtcgtgac gctctccggc gaaaccgatc cggccagccc gtggttcccg
     5461 ttctgggagg ccgtgcccga aggccgcgtc tattacctgc gccggccggc gcgcgccgcc
     5521 gccgacgggc tggccggcat cgtgctcgcg agcggctaca cggtgccgga agcgctgccg
     5581 ccgccggcgc cggaaccgga actgccgccg ccggataccg gcgaaccacc gcccgacacc
     5641 accgtgccgc cacctgaaat cgtgaccgtg gccgtgctgc ccgagtggct gcgcagcgat
     5701 gcgctcgcgc ggctgcggcc ggcgccggaa gcggccactc gcgagcagcg gagcgtgctg
     5761 gcgctgctcg acgaggaaca ggcgagccgc gaaggcacgg ccgccggcta tccgctgacc
     5821 ggctcggtga gcgcggcgct gctgcacatc ggtccgatga ccgattccgc gctgtggccg
     5881 acgctcgccg gactcgccgg cagcggcggg tcgcgcgcca tcgacacctg gctggaaacc
     5941 ttccgccgcg ccgaggccga cggagccgac gtgttccgcg ccggcgtcgc cgccggtcgc
     6001 gaactcggcc tcgacacgac gctgctgcgg ctgtggagca gcatcggctg atgtgcgcga
     6061 tgcccgcccg ttcctgctga agcgcgatgc cggccggcac ggtcctgatc cgtgccggat
     6121 cgcctggagc ctccgatggc cgactttttc cgcctgagcc cgccacagcc gacgccgtcc
     6181 ggccagtcgc gcccgcacct gttcccgctg cgcagcctcg gcgccgccga cttcgaccgg
     6241 ctcgaccgct accgcgacac ccggctcgcg gcgctgctcg ccggccgccc ggccggcatc
     6301 gtcgagggcc tgcgcatcgg cgcacagccc ggcggcgaca gcgtcgtcgt gcgccccggg
     6361 ctcggcatcg ccggcgacgg ccgcacgctg gcgctgcgca cgccgctgcg cgtggcgtgg
     6421 agcgagctga tcggcccggc gcaaggcgac ggcgtgtacc tgatctgcct cgcgcagcgc
     6481 ctgcgccgac aggcgcgcga tgtctccgtg ccctgccggc gcggcgaact cgatccgctg
     6541 cgcgacctgc gcgccgacac cgagagcttc ctgacgctgc gcggcccgct cggcggctac
     6601 gcgcccgagc ccatcgacgg cgacgcggtg ctgatcgcca accgtctggg cacgcgcttc
     6661 ctcgatgcct tcccgcgcgc gacgatcggc gacggcctcg cgctcgggct ggtcgcggtg
     6721 caggccggcg cgccgctgtg ggtcgatgtc gaggccgcgc gcttcgcagc cgtgcccgag
     6781 gccgcgcgcc gcgcgctgct cgaacacacg ctcgccgtga tcgcgcgcgg acgctgggcc
     6841 agcctcgaaa ccgcgccggc cggcgcgccg ctgccgcgcg cctggctgag cctgccggcc
     6901 ccggacggca cgctgccgct gtgtgcgaac ctgcccgcgc acctgcatct gcgcctgctg
     6961 ccggtgcgcg cgagcgagtt tgcgccggcc ctcgccgctg cgcaggcgcg cggcctgctg
     7021 cagcttcgcg acggccgcgc cgatcacgtg catctgctgc tcgcgatccc ggacgccgac
     7081 taccggccgg atctgctgtc gatcccggcc ccggacaccc cgacgctcga tgcgctgtat
     7141 cgcgcctggc tctcggccca gcgcgcctgg gcggcggcgc gcgaggtgcg cgagcagctg
     7201 tggcgcgtga ccgacgagga ccggacctcg ctcggcgacg acgtgctcgc ggcgctgctg
     7261 cccgatcccg atccggtcga gccggcagca ccggccgaac cggcggcgct gctcgccgcg
     7321 ctcgcccagc gccacgccgg ggagacgctg acggcgccgc ccggctacgc gagcttcggc
     7381 gcgtggtccg aagcccatgc caacgacggc ggaccggccg cggtcggctt caccggcagc
     7441 ggggcgcgca tccggctcgc gcgcgcggcg acgcggctgg tgcagctgca gcgcagcctc
     7501 gcggcgcgca ccgaccggct cgacggcatc cgcgacttcc tcggccggct gcgccagcag
     7561 ctcgatgcgc acaccggctc gctggcggcg ctgtccggcg gcgtcgcgag cgacggcagc
     7621 ggcctgcgca tcgcgcgctg gctgccctac gtcagcctga cggccccggc gacgacgatc
     7681 gcggccgccg ccacgccgat cgcagacgcc cggaccttga gcttcgaact gccgctcgcc
     7741 aacgccgcgc cgatcgccga gatcgccgtg cgcccgagct tcacgacgac gctgttcgaa
     7801 acccagggcg tgcaggcggc cggcgtgcag cagcgcttcg atcgcgtgct cgcaagcggc
     7861 ggcggcctga accagcagat ccgcgacacg ctcgcgcaga tcccgcgcgc ggccgaaccg
     7921 gcgaccccgg cggcctttca ggcgcagagc gccgattacg gcgtgatccg ccacgtgctg
     7981 cccgaggtct acgcgctgca ggacgccggc agcaacctcg ccgggctgcg cgacgagatc
     8041 aatgaacgcc tgttcccgga ccgcccgccg ccgaacagcg accgcccgag tccgcgcctc
     8101 gacgccaccg cgatccgccg gctgtacctc gacgtgccgg ccacgcagcg ctatccgcgc
     8161 ctgatcgaac tcggccgcca cctgcacgcc gacacgacgc tgctcgaacg gctgcgcaca
     8221 cgcatggaaa ccgcgatccg ccgccggctg caggagatcg agcagcagaa ggcgctgatc
     8281 gcgcggctgc agcgcgagat cgccgggctc gaagccggac tggccgcgcg ccgccccgaa
     8341 ctcgatgaca cggcgcgcag ctacgcgatg gcgcaggcgc tgaccgccga tgactgggcg
     8401 cgcgtggccg gggcgcgccg gcagcggcgc cgggtactcg cctcggcgcg cggcttcggc
     8461 tacgtgcggg cgctgccgtg gacgatcacg cagcgcccgg ccccggccgt cgcgctcgcg
     8521 gccgtcgagg cggacgaact ggtgcccggc ctgacgcagg cagcggccga aaccctgccc
     8581 gaagcgctcg ccgagtttgc cgaggcgctg ttcgaggtgc cggtcggcga atggcgcagc
     8641 ctgcgcggcc tgctcgtcga actgccgcag cggcgcgtgc tcgacgaggc catcgtgctg
     8701 cgcgcgcagc gcctggggct gcgcagccag cgcacgacca gcagcggccc gcgctatgcg
     8761 gggctcgaac gcgcgagccg cgcgctgtac gcgcaggccg cagtcctcgt gccggtcgat
     8821 gcggccgtgt cactgcagcg ccagcgcgaa caggcggcgc acgcgtattc gctcgaagac
     8881 gcgctgcacg gcccgcccgg ccgcctgcgc cgcgcggccg aggcgctgcg cgcgaagctc
     8941 gaaccggccc tgcgcgccgc ggcctactgg agccggcggc tgccggcccc gctgctgctc
     9001 gaactcgccg atcgcgccga cgaaggcacg ctcgacaccg cccatcccga acgctggccg
     9061 gcaccgccgg ccggcagctc cgagaccgtg ctgaccgact ggcagcgctg gcaggcgctg
     9121 ctgcgctggc tgaacgcgca gctgccggaa ccggccccga ccggtcgcgc cgcgctcgat
     9181 cacctgctgc gcgcgctgct gctcgccgcc gcccatggcg atctcgcgct cgccaccggt
     9241 ggccgggtcg cagccggcgc gacgctgcgc ccgggacaga cgctgcgcct gacgctggag
     9301 cgcgaactgc gccccggcac gcggctggag ttgcgcgacg cgcagcagca gctggtcgga
     9361 cggctgcagc tcgatgacat cgacgcgcaa ggcctcgcca gcgcccgcat cgtcgaggcg
     9421 ctgacaccgg gcgtcgcggt gacgacggcg tttcgcgtct ccaccagcac ctcatgaacc
     9481 cggcggcggc ggcggcacgc agcacgacga tcggccggct gcggctgcgc agcgatccgg
     9541 cgcacgcccc ggcgctgcgc acggcgctgg aactcgccga ctggccgcag cccgaaggcg
     9601 acgcgatcgt gctggtgcgg cgcatcgtcg tgcgcggggc cgcggccagc acgctcgccg
     9661 ccgcggccgg cgtgcggctg gcggccgagc tgcgtgctgc cgtagcgggc gatgcacctt
     9721 cggctgccga tgccggcgcc gtgcgcttca ccggtccggc ggcgctgtac gcccagcttt
     9781 cgcgcgatct cgccgccggc cgcgccgcgc gctgctggta ctgggcacgc tggcgacggc
     9841 tgttcgcgct gccggtgacg agcgcaatcc ccgaactctg gcgcgaacag ccgctgcggc
     9901 tcgccgccat cagcgccgaa ctggcgcgca gcggcgatct ggcccggctg tggcgcgcgc
     9961 tgccgccgca cgaggtacag aggctcgtgc agaccctcgc cgccgccagc ggcctgccgc
    10021 cccccgccga gcctgcggca gggcgcgacg acgaccccgc ttctgcgtca ccgccacccg
    10081 tgccgccgcc ggcgctgctt tcgcgctggc agccggcctg ccgcggcctg ccggccggcg
    10141 atgcgcgcgt gcgtctggcg ctgtatctgc tgctgctcga ctggcgcccg gcgctcgctg
    10201 ccgatgcgac ggcgtgccgg cagctggcgc agcggctgtg cgggatcgtc gatccggaaa
    10261 gaccagcgcc acaccgatcc ctgctgccgg agcagacaca ttcgccggcc ctcccccatc
    10321 cggtcgcggt tgcggttact gcctcggctg ctcacaccga gccggccaaa gcagcagcga
    10381 tccatccggc atccacggac gggcaagaag cgtcggattc gcggacgact gccgtagcga
    10441 aggacgtttt cgtcacgacg ccggactcgc gcctcgcacc ggtctcccgc gaacgcccct
    10501 ccatcgccgt gccggaaacc tgccctgcac cggctcccat gcctgagcct gcgaatcgga
    10561 cctcccgcac ctgcctgccg gacccgtcgc ccggcatcgc cgccagtcat gacgcaggcc
    10621 gcagcggagc gctgcgcgag ccgccggcat cgaccgctgc cctgtcctgc tctgtgcacg
    10681 ccgacaccgg cgtcgacgac tggatcgata ccggcagcgg cggtctgccg tatctgctga
    10741 acgtgctgaa tcaccggcgc gtgcagaccc tgctcggcgg gctgcacggt ctcgatgcgc
    10801 gcggtggcgg ctggcatgcg tggctcgtgc tcggccgtgc gctcgggctg gcacccgacg
    10861 atggcgtcgg gcgctggctc gaacggcgct ggcagctcca cgtcgggcgc gacgaagcct
    10921 ggcagccgca gcccggcgac ccggcactgg tggcgctggc cgcggcgctg tgcgggccgc
    10981 agctctggtc gccggcgctg atcgccggac cgggccggct gcgcgccgac tccacgcacc
    11041 tcgacttcga acggccgctc gccggcgtgc agctcgcggt gcgccgtgcc ggcctcgatc
    11101 tcgatccggg ctggctgccc tggctcgggc gcgtggtcgc gatccgctac gtggaacgct
    11161 ggccgtgaac gcgccggccg tgtccgggct gcccggggaa tcgtcggcgc ggctgtcggc
    11221 ctgggtgcgc accggactca cgtacttcgc cacgcagtgc tacgacgcac tgcccgcgcc
    11281 ggtcacgctg ctcgacgcga gccccgggga ctggccgcag ctctgcaccg ccgtgcccga
    11341 ggccgtcgcc accgcagcag acgcgctggc gcggctggcg cgcgcgtatc gcttgcgccc
    11401 ggccgagctg ttcctgatcg cgctcgccgg cgagatcgag gccgatttcc gcgtcgtgct
    11461 ggcgatcgcc gaactgcagg cgccggagcg cacggcgcgg ccggcgctgc atctggcctg
    11521 cgcgctgacc gaaaccctgt ttccgcagca gaaacgcctg acgccgctca cgctcgccgg
    11581 ctgcaccgcg gtgcgcgccg gactgctcgc gatcgacggc gacggtccgc tgccgatgcg
    11641 cagcctgcgg ctcgatgcgc ggctgtgggc cacgctcggc ggggacgaca cggtctggcc
    11701 ggatacgcag tggctgacgc cggccgatgc cgaactgccg gccgcgaccc gcgcccggct
    11761 cgatccgctc gcccgcgcgc tcgcggacgg agatttcgac gtgctggtgc tgcgcggcat
    11821 gccgtacagc ggccgcggac gctgcgccgc cgcgctggcc gcccggctcg ggctgcaggc
    11881 ctgcgcgatc acgtccgaac gatgggccgc agatgccgcg ctggcggcgg cctgccactg
    11941 ctgcggctgg ctgccggtgc tgcgaccgcg gctggcgccc ggcgaacgct acgaaccccc
    12001 ccggcatccg ggctacgacg gagcgctgat cgtgctgctc ggcgaggccg gagcggtcgc
    12061 cggcacccgg gtgttcgagg aaaccctgcc ggcggcgacc gccggcgagc gcgcggcaag
    12121 ctgggccgca gccggcatcc ccgatccgcc ggcgaccgtg ctcggcggcc cggcgatcgc
    12181 agccctgctg cgctcggccg cctggcaggc cgga
//
LOCUS       DAOQIE010000082        12210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2543_length_12210_cov_7.177869, whole genome shotgun sequence.
ACCESSION   DAOQIE010000082 DAOQIE010000000
VERSION     DAOQIE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_12210_cov_7.177869"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3411
                     /locus_tag="PK144_06930"
     CDS             <1..3411
                     /locus_tag="PK144_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65497.1"
                     /translation="PEPEQDFGFMFGRSERFATRHAGIWAQMANAPAGQVEDAEGLWT
                     WRRISLASTAGKMLSGTAPEFIALTQVPAARLAQQRHEVWLAVGSITAALLLVHSLLS
                     LALAFALAGRARAETGAALAREQALAAVRLQAAQTRYRSVVEGNINGLLVVGPDGRID
                     LVNPALARMFGYTVEDLRGRSIDELLPAAMRTRHVAEREHYMQVPSGRTMGSGRDLYA
                     QRRDGSVFPVEVSLSPFFEDGVTWVQAIVSDITERKHSEALVRKMRDRLQLATDAAGI
                     GVWEWDVVSNTLLWDDWMFRLYHLPEHERIAVLPYQIWHDRCHPGDLERLEALLAAAV
                     AGTDSFDTEFRIVLPSGELRWIRAASVVERDAAGRALRLVGVNQDVTAQRVLETRLLD
                     ARDAAEAASRAKSDFLANMSHEIRTPMNAILGLCQLLLDGELNRRERDYLGKIYTSSR
                     ALLGILNDILDYSKIEAGALTLEVTDFNLDELLDTAADLFSVRTDEKGVELVFDVAPT
                     VPELLYGDPLRLGQVINNLIGNAVKFTERGIIHVRVDIASQGDNHLELRIAVRDTGIG
                     MSGEQCTKLFEAFSQADSSTSRKYGGTGLGLAICRRLVALMGGDIRVDSTPGEGSTFT
                     FTVRLGYGRHALRHRDPSSLRGTRALVVDDQETSRLVLERILESWAFEVTLAASAAEG
                     LAALDAAAAAGRPFGLLLVDWQMPEFDGVAMAREVRRHMASGMLAPAPIVVMVTAYGR
                     EHVLGAVGDGVLDAVLDKPVIPSHLFDTVIGLQHGSTSARQRRVPSGRALQQMTRTIR
                     GARVLLVEDFPTNRLVAQEFLSRMGLIADVAENGREAVDKVATRTYDVVLMDLHMPGM
                     DGLEATRRIRATERGRTLPILAMTAAALAQDREATRAAGMNGHIAKPIDAEELATALL
                     GCIAPRTTTEAGPPPPQTATPPFAIDGLDLTAAVTLSDHDWGFLRRVLQRFQRDFSDA
                     PQRLQAALAAGRHDEAERLVHSIAGLAPGVGSPALGRQGRSLEAALHGGAPIEPMAFL
                     DELQQVLTALAQGLKDVPAPAPAAVADPARVAGLATQLRGLLQEFAIVPLELQQALDA
                     ALAGYTTPALRTALLRQIDQLDYDAAEATLDEILRAGRCDLRAADTTP"
     gene            3442..4365
                     /locus_tag="PK144_06935"
     CDS             3442..4365
                     /locus_tag="PK144_06935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65498.1"
                     /translation="MSPITHLARPAYVLVVDDTPANITLLAEALSPQYRVRFATSGPR
                     ALELIARNDELPDLILLDVMMPEMDGYEVCRQLKEDAATRDIPVIFVSARNEVSSQEH
                     GFNVGGVDYITKPFDLPLVRARVRTHVNLKRRTDLLEQLANLDGLTGIANRRRFDEVL
                     DLEWRRATRTESPLSVVLLDVDHFKLYNDHYGHGLGDDCLRQVAAALAEALPRGGDLA
                     ARYGGEEFAAILPACDVDGAVAVAERIRAAIEQRRIPHATSKTAAFVTISAGCAGLQP
                     LFENRHIELLEAADRALYEAKRAGRNQVCST"
     gene            4735..5622
                     /locus_tag="PK144_06940"
     CDS             4735..5622
                     /locus_tag="PK144_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HNF65499.1"
                     /translation="MPKKLTLAVALSLACGTAAAAPIGYVLGNNGSTLYAFDVSTPGS
                     VLSAVTLSGAVSMLDDIDFRPLNGTLYGYDDGTDQFVTIDTTTGVTTLASTPAAFPTD
                     TRQVGLDFNPTIDRARIVSENEANIVYNPNTGGETLATSLFYVAGDPNNGTNPTVSAN
                     AYTNSMLVGTPGSTVQYVFDAALDILATLGNNAGTLSTIGSLGVDAGQDIGFDILFNG
                     GTGTNTAYGLLTVGGMAGLYTIDLGSGAATLVGNFDGALGTLNGLAITPVPEPASAAL
                     LALGALGLAGARRRSRTAG"
     gene            complement(5844..6380)
                     /locus_tag="PK144_06945"
     CDS             complement(5844..6380)
                     /locus_tag="PK144_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibA"
                     /protein_id="HNF65500.1"
                     /translation="MVLSSLIFGERSLSKVAGLFDTADEARSAANTVRSETGMSDGQV
                     MLVQPNDAAFGRKLEPEQRRIPATLIRTHVVFGAIGLGLGALIALTLMLFGVDAVTSN
                     ALIALVVFSVMGLMLGLMFGGFLSLRPDHDVVISAVMSATRHGHWAVVAHPLDEAQKD
                     SAVRAMEHTHATKVVKTF"
     gene            complement(6634..7620)
                     /locus_tag="PK144_06950"
     CDS             complement(6634..7620)
                     /locus_tag="PK144_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HNF65501.1"
                     /translation="MDKRFVGNLVAIAVAGLVQHAAVAAPLTAGEVLGQFNAVIFGNL
                     NSNSEIEGRTLVGGNLLGTGSTYATRTLPASGYAALAVGGNVVANGYVNVNGGGSAAV
                     AGNVKYMNMNGGTAYIGGNVTGNVNGSKVTGASVTVPDFSQTMLDLAAQLQMLSANSS
                     ISVASNRATFNAAPDADGLAVFDITDSNFFNGIGEIAFNLNGARTVIVNVDLTSATIA
                     ENFLGGAATAVADKVLWNFSAATSLAFNAEFGGSVLAPQASVSNSISLDGTLIAASLT
                     QRGELHQYSFTGTLPGLSAPPATVLRIDEPAGWALLGASAIAGLATRRRRRR"
     gene            8029..10197
                     /gene="uvrD"
                     /locus_tag="PK144_06955"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             8029..10197
                     /gene="uvrD"
                     /locus_tag="PK144_06955"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HNF65502.1"
                     /translation="MDVTPILEHLNDAQRAAVTAPPGPLRVLAGAGSGKTRVLVQRIA
                     WLLAVENASPWAILAVTFTNKAAAEMRARIERQLEFQASGLWIGTFHGIAHRWLRRHW
                     QEAGLAQGFQILDADDQLRVLRRVLRALEIDEKVLTPRQAAWFINAQKDEGRRPQHLD
                     TEDADAARRQLIRIYAAYEQHCAQTGVVDFGELLLRMHETLRDNEPLLRQYRERFRHV
                     LVDEFQDTNRIQYAWLRLLTGGSGDVFIVGDDDQSIYGWRGARIENLQRFEQDFPGAQ
                     TVRLEQNYRSSATILKAANALIARNAGRLGKTLWTDGADGVPIDFYAAYNDYDEARFV
                     VERIEQWVGAGGRRADNAILYRSNAQSRLFEEALLARGMPYRVYGGQRFFERAEIKDA
                     LAYLRLLVNRHDDASFERVVNVPLRGLGERTLEELRTQARLDGSSLWTAAVGLLAAGR
                     APARAGKALAGFLQLIERLAADCAPLALGERTAAMLDASGLREHVRKDRDGKAEDRLE
                     NLDELISAARAFETLPPEYEGLTPVDAFLAHASLEAGDAQAEAWSDCVQLMSLHSAKG
                     LEFPQVFIAGVEEELFPHALSMQDPEKLEEERRLAYVGITRARERLVLSCAERRRLHG
                     QEHYPRPSRFLSEIPPGLLREVRARAQISRPVYQPPVAAAPDDGGSGLKPGARVRHPS
                     FGEGVVLTLEGSGAHLRVQVNFPGQGARWLVAAYAKLEVR"
     gene            10292..10576
                     /locus_tag="PK144_06960"
     CDS             10292..10576
                     /locus_tag="PK144_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65503.1"
                     /translation="MRNRLVHIAPLQTAKVLAAIYFVISVPIALLMLIPVLLGFGQQG
                     AGMGLLMVILTPFLYALAGFLFTLLGAWIYNLVAARLGGIEYTTITAVER"
     gene            complement(10665..11141)
                     /locus_tag="PK144_06965"
     CDS             complement(10665..11141)
                     /locus_tag="PK144_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65504.1"
                     /translation="MKWADLHQGQWIHGKRIGSVDNLGGMHGLPEQAVLAQLLNKAQN
                     LNLKPVYTGMSFTWCRAVVTNHQVSCQNESSQTDTDSKSPEICHEDRVFPVSPGDGHL
                     LIRQNLAPCARCRAAFRAWAKSRGSTIIVVSDKGYDSSPDNTIFIFGPTGAAYQWY"
     gene            11528..12004
                     /locus_tag="PK144_06970"
     CDS             11528..12004
                     /locus_tag="PK144_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65505.1"
                     /translation="MSESNRVSAVLGDAEIAELRTQIAALRARLPFLVNLSPQDRKEL
                     PKLGDKTVGFDEKCQTYMESNPEFLPGFVDTAELDRDRALRSQMLRIAPDLLLLAEQI
                     EDTLTVLGSEIVMVDFAYYQGVREAARRGLPGAQLIYDDLRTRFPGPGRPSLKTKT"
BASE COUNT         1889 a         4257 c         4222 g         1842 t
ORIGIN      
        1 cccgagccgg aacaggactt cggcttcatg ttcgggcgca gcgagcgctt cgcgacccgc
       61 cacgccggca tctgggcgca gatggcgaat gcgccggccg ggcaggtcga ggatgcggag
      121 ggcctgtgga cctggcggcg gatcagtctc gccagcacgg cgggcaagat gctgagcggc
      181 acggccccgg agttcatcgc gctgacgcag gttccggcgg cacggctggc gcagcagcgt
      241 cacgaggtct ggctggcggt cggcagcatc accgccgcgc tgctgctggt gcattcgctg
      301 ctgtcgctcg cactcgcctt tgcactggcc gggcgggcgc gcgccgaaac cggcgcggcg
      361 ctggcgcgcg agcaggcgct cgccgccgtg cgcctgcagg cggcgcagac gcgctaccgc
      421 tcggtggtcg agggcaacat caacggcctg ctggtggtcg ggccggacgg gcgcatcgat
      481 ctggtcaatc cggcgctggc gcgcatgttc ggctacacgg tcgaggatct gcgcgggcgc
      541 agcatcgacg aactgctgcc ggcggccatg cggacacgcc acgtcgcgga gcgcgagcac
      601 tacatgcagg tgccgagcgg gcgcacgatg ggcagcggcc gcgacctgta cgcgcagcgg
      661 cgcgacggca gcgtgttccc ggtcgaggtc agtctgagcc cgttcttcga ggacggcgtg
      721 acctgggtgc aggccatcgt cagcgacatc accgagcgca agcactccga ggcgctggtg
      781 cgcaagatgc gcgaccggct gcagctggcc accgatgccg ccggcatcgg tgtctgggag
      841 tgggatgtcg tcagcaacac gctgctgtgg gacgactgga tgttccggct gtaccacctg
      901 ccggagcacg agcgcatcgc cgtgctgccc taccagatct ggcacgaccg ctgtcatccc
      961 ggggacctcg aaaggctgga ggccctgctc gcggcagcgg tggccggaac cgacagcttc
     1021 gacaccgagt tccgcatcgt gctgccctcg ggcgagctgc gctggattcg cgcggcctcg
     1081 gtcgtcgagc gcgatgccgc aggccgggcg ctgcgcctgg tcggcgtcaa tcaggacgtc
     1141 accgcccagc gcgtgctcga aacccggctg ctcgatgcgc gcgatgccgc cgaggcggcg
     1201 agccgggcca agtccgactt cctcgccaac atgagccacg agatccgcac gccgatgaac
     1261 gcgatcctcg ggctctgtca gctgctgctc gacggcgagc tgaaccgccg cgagcgcgac
     1321 tacctcggca agatctacac ctcctcaagg gcgctgctcg gcatcctcaa cgacatcctc
     1381 gactattcga agatcgaggc cggggcgctg acgctggaag tcaccgactt caacctcgac
     1441 gagctgctcg acaccgctgc cgacctgttc tcggtgcgca ccgacgagaa gggcgtcgag
     1501 ctggtgttcg acgtggcgcc gaccgtgccc gagctgctgt acggcgatcc gctgcgcctc
     1561 ggccaggtca tcaacaacct gatcggcaac gccgtgaagt tcaccgagcg cggcatcatc
     1621 cacgtgcggg tcgacatcgc cagccagggc gacaaccacc tggagctgcg catcgccgtg
     1681 cgcgacaccg gcatcggcat gagcggcgag cagtgcacga agctgttcga agccttctcg
     1741 caggccgaca gctcgacctc gcgcaagtac ggcggcaccg gcctcggact ggcgatctgc
     1801 cggcggctgg tggcgctgat gggcggcgac atccgcgtcg acagcacgcc gggcgagggc
     1861 agcaccttca cgttcacggt gcgcctcggc tacggccggc acgccctgcg ccaccgcgac
     1921 ccgtccagcc tgcgcggcac gcgggcgctg gtggtcgatg atcaggaaac ctcgcggctg
     1981 gtgctggagc gcatcctcga atcctgggcc ttcgaggtga cgctggccgc cagcgccgcc
     2041 gaaggtctgg ccgcgctcga tgccgcggcc gctgccggcc ggccgttcgg gctgctgctg
     2101 gtcgactggc agatgccgga gttcgacggc gtggcgatgg cgcgcgaggt gcgcaggcac
     2161 atggccagcg gcatgctggc tccggccccg atcgtggtca tggtcaccgc ctacggccgc
     2221 gagcacgtgc tcggcgccgt cggcgacggc gtgctcgatg ccgtgctcga caagccggtg
     2281 atcccctcgc acctgttcga taccgtcatc ggcctgcagc acggcagcac gtcggcaagg
     2341 cagcgccgcg tgccttccgg ccgggcgctg cagcagatga cgcgcacgat ccgcggcgcc
     2401 cgcgtgctgc tggtcgagga cttcccgacc aaccggctgg tggcgcagga gtttctgtcg
     2461 cgcatggggc tgattgccga tgtcgccgaa aacggccgcg aagccgtcga caaggtcgcg
     2521 acccggacct acgacgtggt gctgatggac ctgcacatgc ccggcatgga cggcctcgaa
     2581 gccacgcgcc gcatccgcgc caccgagcgc gggcgcacgc tgccgatcct cgcgatgacc
     2641 gccgcggcgc tggcgcagga tcgcgaggcg acgcgcgccg ccggcatgaa cggccacatc
     2701 gccaagccga tcgatgccga ggaactggcc acggcgctgc tcggctgcat cgccccgcgc
     2761 accacgaccg aagccgggcc gccgccgccc cagaccgcaa cgccgccgtt tgcgatcgac
     2821 ggcctggacc tgacggcggc cgtcacgctg agcgatcacg actggggctt cctgcgccgc
     2881 gtgctgcagc gcttccagcg ggatttcagc gacgcgccgc agcgcctgca ggcggcgctg
     2941 gccgcaggcc ggcacgacga ggccgagcgg ctggtgcaca gcatcgccgg gctggcgccg
     3001 ggcgtcggca gtccggcgct gggccgccag ggccggagcc tggaagccgc cctgcacggc
     3061 ggcgcgccga tcgaaccgat ggcgtttctc gatgagctgc agcaggtgct gacggcactg
     3121 gcgcaggggc tgaaggacgt gccggccccg gccccggccg cggtggccga tcccgcccgg
     3181 gtggcgggcc ttgccacgca gctgcgcggc ctgctgcagg agttcgcgat cgtgccgctg
     3241 gagctgcagc aggcgctcga tgcggcgctg gccggctaca cgacgccggc gctgcgcacc
     3301 gcgctgctgc gccagatcga ccagctcgac tacgacgcgg ccgaggccac gctcgatgaa
     3361 atcctgcgtg ccggccgctg cgacctgcgg gccgccgata cgaccccctg attccgattt
     3421 ccgaaccgac gcgtacccga gatgagcccc atcacccacc tcgcccgacc ggcctacgtg
     3481 ctggtcgtcg atgacacccc ggccaacatc acgctgctcg ccgaggcgct gagcccgcag
     3541 taccgcgtgc gcttcgcaac cagcggtccg cgggcgctgg agctgatcgc ccgcaacgac
     3601 gagctgccgg acctgatcct gctcgacgtg atgatgccgg agatggacgg ctacgaagtc
     3661 tgccgccagc tcaaggaaga cgcggcgacc cgcgacattc cggtgatctt cgtctcggcg
     3721 cgcaacgagg tgtcgagcca ggagcacggc ttcaacgtcg gcggcgtcga ctacatcacc
     3781 aagcccttcg acctgccgct ggtgcgcgcg cgcgtgcgca cgcacgtcaa cctcaagcgc
     3841 cgcaccgacc tgctggagca gctcgccaac ctcgacggcc tgaccggcat cgccaaccgc
     3901 cgccgcttcg acgaggtgct cgacctcgaa tggcggcgcg cgacgcgcac cgaaagcccg
     3961 ctgtcggtgg tgctgctcga tgtcgatcac ttcaagctct acaacgacca ctacggccac
     4021 ggcctcggcg acgactgcct gcgccaggtg gcggcggcac tggccgaggc gctgccgcgc
     4081 ggcggcgatc tggccgcgcg ttacggcggc gaggagttcg cggcgatcct gccggcctgc
     4141 gatgtcgatg gcgcggtcgc ggtcgccgag cgcatccgcg ccgcgatcga gcagcggcgc
     4201 atcccgcacg ccacctcgaa gacggccgcg ttcgtgacca tcagtgccgg ctgcgccggc
     4261 ctgcagccgc tgttcgagaa tcgccacatc gaactgctcg aagccgccga ccgcgcgctg
     4321 tacgaggcca agcgcgccgg ccgcaatcag gtctgcagca cctgacggga ccggcgtcgc
     4381 ccgcatcgcc gacggcacgc ggggccggac aacgggccgg atgcatcccg gatgcatccg
     4441 gccctgattt tttccggcgc tttccggcgg cgttgggccg gcagggcggt gtccgcagcg
     4501 acgacggcgc agcgggcagg cggaaaccgt ccgcagcgtt gcggcagccg gctgatccgc
     4561 aagggttccg gccgcggatt ccgggcgggc tgcgacggtc tggtgcggga ttcatcacgc
     4621 gtgatgaatt ttgcgtcatg ttgcatccgt ctgcggcggc ggtccgcatc cggggaggcg
     4681 tgacccgtcc gcagggtccg caggaagtct gtccctcact cccgggagcc cgccatgccc
     4741 aagaaactga ccctcgccgt cgccctgagc cttgcctgcg gcacggccgc cgctgcgccg
     4801 atcggctacg tcctcggcaa caacggcagc acgctgtacg cgttcgacgt gagcacaccc
     4861 ggctccgtgc tgagcgcggt gacgctgagc ggtgccgtgt cgatgctcga tgacatcgac
     4921 ttccggccgc tgaacggcac gctgtacggc tatgacgacg gcacggacca gttcgtgacc
     4981 atcgacacca cgaccggcgt cacgacgctc gccagcacgc cggcagcctt cccgaccgac
     5041 acccgccagg tcggcctcga cttcaacccg accatcgacc gggcgcgcat cgtcagcgag
     5101 aacgaggcca acatcgtcta caacccgaac accggcggcg agacgctggc gaccagcctg
     5161 ttctacgtcg ccggggatcc caacaacggc acgaatccga cggtcagcgc caacgcctat
     5221 accaacagca tgctggtcgg cacgccgggc tcgacggtgc agtacgtgtt cgatgccgcg
     5281 ctcgacatcc tcgccacgct cggcaacaac gccggcacgc tgagcacaat cggcagcctc
     5341 ggcgtcgatg ccggtcagga catcggcttc gacatcctct tcaacggcgg caccggcacg
     5401 aacaccgcct acggcctgct gacggtcggc ggcatggccg ggctgtatac gatcgacctc
     5461 ggcagcggtg cggcaacgct ggtcggcaac ttcgacggcg ccctcggcac gctgaacggc
     5521 ctggcgatca cgccggtgcc ggagccggct tcggcggcgc tgctggcgct cggcgcactc
     5581 gggctggccg gcgcacgccg ccgcagccgc acggccggct gatatcagtc accactgaac
     5641 atccggtctt ttcagcccct cccccctcgc gggggagggg ttggggagag ggggatcaac
     5701 aacgcattca cgtgtgatcg gtatgacccg ccatgcacgg cggggaccgg atcgtcgccc
     5761 gccgtgccgc catcgcgctc atgaaaaagc ccacccccgt ccggggatgg gccgcatggg
     5821 aaagacggga tcagggggcc ggatcagaag gtcttgacga ccttcgtcgc gtgcgtgtgc
     5881 tccatcgccc gtaccgcgct gtccttctgc gcctcgtcga gcggatgcgc gaccaccgcc
     5941 cagtggccgt ggcgggtcgc cgacatcacg gcgctgatga ccacgtcgtg atccgggcgc
     6001 agcgacagga agccgccgaa catcaggccg agcatcaggc ccatgacgct gaacaccacc
     6061 agcgcgatca gcgcgttcga ggtcacggca tcgacgccga acagcatcag cgtcagcgcg
     6121 atcagcgccc cgagaccgag accgatggcg ccgaacacga cgtgcgtgcg gatcagcgtc
     6181 gccgggatgc gccgctgctc gggttcgagc ttgcggccga aggcggcatc gttgggctgc
     6241 acgagcatga cctgcccgtc ggacatgccg gtttcgctgc gcaccgtgtt cgcggcggag
     6301 cgggcttcgt cggcggtatc gaacaggccg gcgaccttgg acagggagcg ttcaccaaag
     6361 atcagggacg aaagaaccat cgcaacacct ccacgggggg tatggggcgg aatcggacgg
     6421 cgtccggagc gcaccggctc cgggccggct gatccgtctg cgcgctgacg caaccggctt
     6481 gcgtggccgg gcatctgcgc gctgtgaccg ccgggatcgc tgccgcgttc cggaagaggc
     6541 gcagcccgtg gcctgctcag gagggaagga cgcggaaaac ggaacggccg ctcccgggcg
     6601 ggggcggccg tggcgggcgg gaaaccgggc ggttcagcga cggcggcgac ggcgtgtcgc
     6661 gaggccggcg atggccgagg cgccgagcag cgcccagccg gccggttcgt cgatgcgcag
     6721 caccgtcgcc ggcggcgcgc tcaggcccgg cagcgtgccg gtgaacgaat actggtgcag
     6781 ttcgccgcgc tgggtcagcg acgcggcgat cagcgtgccg tcgaggctga tgctgttgct
     6841 gaccgaggcc tgcggcgcca gcacgctgcc gccgaattcg gcgttgaagg cgaggctggt
     6901 ggcggcgctg aaattccaca gcaccttgtc ggcaaccgcg gtggcggcgc cgccgaggaa
     6961 attctcggca atcgtcgccg acgtgagatc gacgttgacg atgacggtgc gcgcgccatt
     7021 gaggttgaag gcgatttcac cgatgccgtt gaagaagttg ctgtcggtga tgtcgaacac
     7081 cgcgagcccg tcggcatccg gcgcggcatt gaaggtggcg cgattgctgg cgacgctgat
     7141 cgagctgttg gcgctcagca tctgtaattg cgcggccaga tcgagcatgg tctggctgaa
     7201 atccggcacc gtgacgctgg cgccggtcac cttgctgcca ttgacgttgc cggtgacgtt
     7261 gccgccgata tacgcggtgc cgccattcat gttcatgtac ttgacgttgc cggcgaccgc
     7321 cgcgctgccg ccgccgttga cgttgacata gccattggca acgacgttgc cgccgaccgc
     7381 cagcgccgcg tagcccgagg ccggcagcgt gcgcgtcgcg taggtcgagc cggtgccgag
     7441 caggttgccg ccgaccagcg tgcggccctc gatctcggag ttcgagttca ggttgccgaa
     7501 gatcacggca ttgaactggc ccagtacctc gccggcggtc agcggtgcgg cgacggccgc
     7561 gtgctgcacg aggccggcca cggcgattgc aacgagattg ccaacgaaac gcttgtccat
     7621 gtcacacctg cctgcaggaa atcgatgccg tgcaccgtcg ttctggtgct ttgcgtcatt
     7681 tcagcgatcc ggcagcaaat gcaataccgg cgggcggtga cggatcgtga cgtgcaacca
     7741 ttcgttgaat tcgcgtgcga attgctgcgt gccggttcgt ggcgtctggt gacgccgaaa
     7801 cacgggtttg agattcggcc gccggatggt cttttccgct gcgctcgatc tgcggtaaaa
     7861 gcctttccgg attcctgcgg attgcatcgg ctgcaatctg cgaatgcagg atgcgaatgt
     7921 gaatccggcg cggcaggaat tgtttcgggc gcggtgcgcc cggccgcgtc agcgccgtgg
     7981 ccgggcaggg cggccagcgc ggcgcccgat gccggtacaa tcgccggcat ggacgtgacc
     8041 cccatcctcg aacacctcaa cgatgcgcag cgcgccgcgg tcaccgcgcc gcccggaccg
     8101 ctgcgcgtgc tcgccggtgc cggctccggc aagacccgcg tgctggtgca gcgcatcgcc
     8161 tggctgctgg cggtcgagaa cgccagtccg tgggccatcc tcgccgtcac cttcaccaac
     8221 aaggccgcgg ccgagatgcg cgcgcgcatc gagcgacagc tcgaattcca ggcctccggg
     8281 ctctggatcg gcaccttcca cggcatcgcg caccgctggc tgcgccggca ctggcaggag
     8341 gccgggcttg cgcagggctt ccagattctc gatgccgacg accagctgcg cgtgctcagg
     8401 cgcgtgctgc gtgcgctcga aatcgacgag aaggtgctca cgccgcgcca ggccgcatgg
     8461 ttcatcaacg cgcagaagga cgagggccgc cggccgcagc acctcgacac cgaggacgcc
     8521 gacgctgcgc gccgccagct gatccgcatc tatgccgcct acgagcagca ctgcgcgcag
     8581 accggcgtcg tcgatttcgg cgagctgctg ctgcgcatgc acgagacgct gcgcgacaac
     8641 gagcccctgc tgcgccagta ccgcgagcgc ttccggcacg tgctggtcga cgagttccag
     8701 gacaccaacc gcatccagta cgcctggctg cggctgctga ccggcggcag cggcgacgtg
     8761 ttcatcgtcg gcgacgatga ccagtcgatc tacggctggc gcggcgcgcg catcgagaac
     8821 ctccagcgct tcgagcagga cttccccggc gcgcagaccg tgcgcctcga acagaactac
     8881 cgttcgagcg cgacgatcct gaaggccgcc aacgcgctga tcgcccgcaa cgccgggcgg
     8941 ctcggcaaga cgctctggac cgacggcgcc gacggcgtgc cgatcgactt ctacgccgcc
     9001 tacaacgact acgacgaggc gcgcttcgtc gtcgagcgca tcgagcagtg ggtcggcgcc
     9061 ggcggccggc gcgccgacaa cgccatcctg taccgctcga acgcgcagtc gcgcctgttc
     9121 gaggaagccc tgctcgcgcg tggcatgccg tatcgcgtct acggcggcca gcgtttcttc
     9181 gagcgcgccg agatcaagga cgcgctcgcc tacctgcgcc tgctcgtcaa ccgccacgac
     9241 gacgcctcgt tcgagcgcgt cgtcaacgtg ccgctgcgcg gcctcggcga gcgcacgctc
     9301 gaagaactgc gcacgcaggc gcggctggac ggcagctcgc tgtggacggc cgccgtcggc
     9361 ctgctcgccg ccggccgcgc cccggcccgc gccggcaagg cgcttgcggg ctttttgcag
     9421 ctcatcgagc ggctcgccgc cgactgcgcg ccgctcgcgc tcggcgagcg caccgccgcg
     9481 atgctcgatg ccagcggcct gcgcgagcac gtgcgcaagg atcgcgacgg caaggccgag
     9541 gaccggctcg aaaacctcga cgagctgatc agcgccgcac gcgccttcga gaccctgccg
     9601 cccgagtacg agggcctgac gccggtcgat gccttccttg cgcatgcctc gctggaggcc
     9661 ggcgacgcgc aggccgaggc ctggagcgac tgcgtgcagc tgatgagcct gcacagcgcc
     9721 aaggggctgg agttcccgca ggtgttcatc gccggcgtcg aggaggagct tttcccgcac
     9781 gcgctgtcga tgcaggaccc ggaaaaactc gaagaggaac ggcgcctcgc ctacgtcggc
     9841 atcacccgtg cgcgcgaacg cctggtgctg tcctgcgccg agcgccgccg gctgcacggt
     9901 caggagcact acccgcggcc ctcgcgcttt ttatcggaaa tcccgcccgg gctgctgcgc
     9961 gaggtgcgcg cccgcgcgca gatcagccgc ccggtctacc agccgccggt cgccgccgcg
    10021 cccgatgacg gcggcagcgg cctcaagccc ggcgcgcgcg tgcgtcatcc gagctttggc
    10081 gagggcgtcg tgctgacgct cgaaggcagc ggcgcccacc tgcgcgtgca ggtgaacttc
    10141 cccggccagg gcgccaggtg gctggtcgcg gcctacgcga agctcgaagt gcggtaggcc
    10201 ccggcccggc tgcctatgct gcggacagcc ccgcccgtcg aggcgggcgg tttcttctgt
    10261 ccgaaatgct gtccgaaccg aggcccttcc gatgcgcaac cgtctggtcc acatcgcccc
    10321 gctgcagacc gccaaggtgc tggcggcgat ctatttcgtc atctcggtgc cgatcgcgct
    10381 gctgatgctg atcccggtcc tgctcggctt cggccagcag ggcgccggca tggggctgct
    10441 gatggtgatc ctgacgccat tcctgtacgc gctcgccggc ttcctgttca cgctgctcgg
    10501 cgcctggatc tacaacctcg tcgccgcccg gctcggcggc atcgagtaca cgacgatcac
    10561 ggcggtcgaa cgctgaggaa ccggggccgg agccggcgct ccggtcctgc gccttgcagg
    10621 cattggcggc tgcgcagctt cagcgagttt cgccggccgc ggcatcagta ccactgatac
    10681 gcggcgccgg tcgggccgaa gatgaagatg gtgttgtccg gcgagctgtc gtagcccttg
    10741 tccgaaacca cgatgatcgt cgagccccgg ctcttggccc atgcccggaa ggcggcgcga
    10801 cagcgcgcgc agggcgcgag gttctggcgg atcagcagat gcccgtcgcc gggcgaaacc
    10861 ggaaacacgc ggtcctcgtg gcagatttcc ggggatttgc tgtcggtgtc ggtctgcgag
    10921 ctttcgttct ggcaggacac ctgatgattg gtgacgacgg cccggcacca cgtgaaggac
    10981 atgccggtat agaccggctt cagattcagg ttctgcgcct tgttcagcag ttgcgccagc
    11041 accgcctgtt cgggcaggcc gtgcatgccg ccgagattat cgacgctgcc gatgcgcttg
    11101 ccgtgaatcc actggccctg atgcaggtcg gcccatttca tgatctgtct ccggttacgg
    11161 gtcgggggat gcgcttcgtg caggccgtgc cgacgcgcgc cgcggcgcgg gtcgatgcgg
    11221 tccatgcctg ccgatacggg gccggaggcg atccggagac aggggcggcg cagatttccc
    11281 gagcgccggt cgcagttgca ggccgatgca ggcggcggat gcgccagcag cggccgcagc
    11341 ctgacaccac cggcccgctg tgtttcgcgt ccggcaacag gaggcgcaat gctgcggcgc
    11401 cgactacgct cgcagcctca gggatccatg actgccgcga ggggcaaggg aggggagggc
    11461 accgcaggac ggccgttggt gcgcggaacc gggacgtttc cgaatcatcc tcagtcagga
    11521 ccatgccatg agcgaatcga atcgtgtttc cgccgtgctc ggcgatgcgg agattgccga
    11581 gttgcgcacg cagattgctg ccttgcgcgc cagattgccg tttctcgtca acctcagccc
    11641 gcaggaccgc aaggaactgc cgaagctcgg cgacaagacc gtcggcttcg acgagaaatg
    11701 tcagacctac atggaatcga atccggagtt cctgccgggt ttcgttgata ccgctgaact
    11761 cgaccgggat cgcgccttgc gcagccagat gctgcgcatc gcgcccgatc tgctgttgct
    11821 ggccgagcag atcgaggaca cgctgaccgt gctcggcagc gagatcgtca tggtggattt
    11881 cgcctactac cagggcgtgc gcgaggcggc gcgccgcggc ctgccggggg cgcaactgat
    11941 ctacgacgac ctgcgtactc gcttccccgg ccccggccgc ccgtccttga aaaccaagac
    12001 gtaatgcacc ccgccatcgc cggtcgcggt cgccgcgcgc gacccggccg gcggcggtgg
    12061 cggtctgtcc ggcgcagggt ttcaggctgt ttttgcacta tataagtgca aaaccgaggt
    12121 ttttcagcct cccggtcgcc tgcagagccc cgaaaacctc gttccgaacg acctcgacct
    12181 tgttccgaac gaggtcgtcg cagcaaaaag
//
LOCUS       DAOQIE010000083        12187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2552_length_12187_cov_7.514590, whole genome shotgun sequence.
ACCESSION   DAOQIE010000083 DAOQIE010000000
VERSION     DAOQIE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2552_length_12187_cov_7.514590"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK144_06975"
     CDS             complement(<1..207)
                     /locus_tag="PK144_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNF65506.1"
                     /translation="MNMPTPTSEAAAAAALPGSLAPPGLPDVAGLTQLASAFFAMLPG
                     ETMAAALPGLAQLAPSLTAAQPPAS"
     gene            complement(182..1111)
                     /locus_tag="PK144_06980"
     CDS             complement(182..1111)
                     /locus_tag="PK144_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNF65507.1"
                     /translation="MSDSSHVHSALGDHAARQLANATKTVPQLSTITPRWLVHLLQWV
                     PVEAGIFRLNKVSNPTSVEVACSQRDEAALPKTFVDYEEQPREYFLNAVTTVLDVHTR
                     VSDLYSSPHDQIREQLRLTIETIKERQESELINNPDYGLLANVADAQRLSTLNGAPTP
                     DDFDELITKVWKAPGFFLAHPLAIAAFGRECTRRGVPPATVSLFGCQFITWRGLPLIP
                     SDKIPVVDGRTQILLLRTGESRQGVIGLYQPGLTGEQSPGLSVRFMGIDRAAIASYLI
                     SLYCSLAILTDDAVAVLEDVEVGKFHEYAHTYQ"
     gene            complement(1536..2453)
                     /locus_tag="PK144_06985"
     CDS             complement(1536..2453)
                     /locus_tag="PK144_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNF65508.1"
                     /translation="MNRRDDEVPVAPEAAQSWDIGPIVRELRAQRERWRTARQRRLTA
                     GGREFPARAAIERILADLCGALFPMRLGPDDLQRESEDYYVGHTLDRALRALLGQVCL
                     ELGVASAEAVHHVRRFAEALPGIRALLDADVEAAFAGDPAARSVDEVLLCYPGVLAII
                     HHRLAHQLHGQGLALLARIVAEIAHAQTGIDIHPGARIGRSFFIDHGTGVVIGETAVI
                     GDHVRLYQAVTLGARSFQTAIDGSLEKGLARHPVVEDDVVIYAGATILGRITIGRGSV
                     IGGNVWLTHSVPPGSHVTQASTRQEQVNH"
     gene            2726..2974
                     /locus_tag="PK144_06990"
     CDS             2726..2974
                     /locus_tag="PK144_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65509.1"
                     /translation="MHRQAFRQSSGSGQFHDRRQASAITDAIRIYAAELPACEMNAIL
                     MNAILMNVIFMNPESSRINPDQAVRATGTGTLTPAPRL"
     gene            complement(3049..3525)
                     /locus_tag="PK144_06995"
     CDS             complement(3049..3525)
                     /locus_tag="PK144_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF65510.1"
                     /translation="MTRALPELSAALIDAEAVLASARPAEPAGYAGGLSPVDAWTLFA
                     TGHAVLVDVRTVEELAYVGRVPGSLHVPWATGTAQTRNPRFLREFEKRVPQKDDVVLL
                     LCRSGKRSVAAAAALAAAGYTRAYNVLEGFEGERDADGHRGGQGGWRHHGLPWEQD"
     gene            complement(3923..4531)
                     /locus_tag="PK144_07000"
     CDS             complement(3923..4531)
                     /locus_tag="PK144_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNF65511.1"
                     /translation="MHLVTLAGCTEPDARPQQVLDWLADWLGHYGLPVRAIRPGLLPA
                     EDLLHARREAPAIRAAVQVLAGAQALLIATPVRLGTYTGVLKTFLDLLPAGALAGVRV
                     LPLACGGEASRQLTLHYALKPVLDALGASGVLSSLQIGARQARLHDDGLLVLDPGVET
                     RIEAALLRLLAGLPLAASLPTDALPPPPRALRRLPSAGRPAG"
     gene            complement(4680..7067)
                     /locus_tag="PK144_07005"
     CDS             complement(4680..7067)
                     /locus_tag="PK144_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65512.1"
                     /translation="MPLRPLTVLLIAATAAAALTLAAVLGNTWRVQSQLETRLHALAG
                     RERAGLLARALEPALAQRNAEAAQAALAPLSADPALRYAAVFTPERRVLARIGDAPGA
                     DPAVPPPGEYRTSSVDGVLRSELPVVAGTSGRLAGFVWIGLDPSAYGQDNTLLLQNAG
                     IVLGGLVLTGFALLRLGSELRGGLGHVRRGLDALAREDYELRLPALPAGDLARIGETV
                     NLLAARLGERANSARSQQMQLRQESRRLGAMLRGISAVVWEAEPRSGRFRYVSPEAEV
                     LLGHSPSQWLAPDFLDRCCHAADREWAQGFLGHAGASARSHTMDLRLRHRDGSYRWLR
                     LMGSGEQTAEGPILTGLLLDVTEEKLAEQRVAFLADHDSLTGLVNRRRFQELLQNHID
                     AARQPGASPGALLFLDLDQFKYINDSYGHHTGDEYLRQVTQHLRVPLAPEHVLGRLGG
                     DEFGVILPGTDPDSSELLCRTLLATLNAQEFIHQGRSTPFPASIGVAYFPQHGIHTSE
                     LLANADTAMYEAKAHGRNTWCVFEGGGDLARMREKIHWEDRIRTALRDHRLMLVFQPI
                     VDLRTGKVVHYESLLRMLGDQGETIGPNAFIAVAERFGLIRELDRWVVETAIATQGAS
                     RLRNDPVSLTINLSARHFGSADMLELIRDATHRHRADPGAIVFEVTETAAVENFTAAR
                     EFIQALRNAGYRFALDDFGAGFSSFHYLKNLTVDYVKIDGSFVRHLGTDRADRIFVKA
                     IADLAIGLGIRPIAEFVENRATVEHLLELGIPLGQGMYFAAPTSEFIAPALRATA"
     gene            complement(7128..7451)
                     /locus_tag="PK144_07010"
     CDS             complement(7128..7451)
                     /locus_tag="PK144_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNF65513.1"
                     /translation="MSPEAACWRWEGADLVLGVRVQPRASRDRLEEVRDGCLRVRLCA
                     APVDGQANARLCALLADSFGVPKSAVELLAGDAARVKRLRIRAPRRLPEVLLAAGLCA
                     PAAGA"
     gene            complement(7468..8046)
                     /locus_tag="PK144_07015"
     CDS             complement(7468..8046)
                     /locus_tag="PK144_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNF65514.1"
                     /translation="MGGTPYFSEAGVFLIRTVFGFYLLALMLRFLLQWVRADFYNPLV
                     QFLVRITNPPLLPLRRYVPGLYGLDMACVLLMLLLAILEWGLILSMVGRSFGPLGLLL
                     LGLVELLGLTLDVFLWAVIIQALMSWFVRDPRNPLYGLLYRLTAPVLRPAQRVLPLVG
                     GVDLSPLLVIVVLQLASILLIAPLRDLALALH"
     gene            complement(8046..8900)
                     /gene="proC"
                     /locus_tag="PK144_07020"
     CDS             complement(8046..8900)
                     /gene="proC"
                     /locus_tag="PK144_07020"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNF65515.1"
                     /translation="MIRTPIAFIGGGNMARSLIGGLLADGCPAALLRVAEPSAGGREA
                     LRAQYGVQTSEHNPDVIDGAGVVVLAVKPQILREVALGIAEAVQHARPLVVSIAAGVR
                     LADIGRWLGGHGALVRTMPNTPALVKSGATALYAAAGVSVEQRRLAESILRAVGLTVW
                     VDDEARLDAVTALSGSGPAYFFLVMEAMEAAARTLGLPADTARLLTLQTAFGAARIAL
                     ESSESPAELRARVTSPKGTTEAALQVLKNAGIEAIFAEALAAAAQRSRELGDLFADTP
                     AAGGGEGT"
     gene            complement(8992..10722)
                     /locus_tag="PK144_07025"
     CDS             complement(8992..10722)
                     /locus_tag="PK144_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF65516.1"
                     /translation="MSVRMMALALIVALGPALPAAAAPPMMKWQDEQGRWHYGDRPPQ
                     AGARADALLETGQDAAAAKEYETWAKMGDARAQTKLGELYLEGRGVSRNEAKARELFG
                     IAAGNGYAEARFRLGDLLATGRGGALDLPGAVRWYRQAAEGGHAGAQGRYGTALVDGQ
                     GVARDVAQGLQWLMRGAEAGDADAEYRLGLCYLRGEGVLGDPRTAVEWFRRAATRGQP
                     GAAQQLAELAASGRVPGLELAEVMPGLVRAAESGFPPAQLSLGLIHLQGLTGTQNLAL
                     AVRWLGAAAEAREPLAQFTLAGLYANGEGVARDERRAVQLYRGAAEAGHPRAQFELAE
                     RLAKGAGVPRDDAEAVVWYARAAKQGLASAHTRLGEHYQRGRGIAADLLLAVREYRAA
                     AEAGHAPAQLALALLTRAGTGTERDPELAVLWLRRAADQGLGLAQVDLGLAYAEGSGV
                     ARDDEQAARWYARAARAGYAPGRFLLGMASLTGRGVQRDEAQAAVLLRAAAEQGYAPA
                     QTAYGLLLRDGRGMPADPVAAWKWLDLAALDGDQVALQARETLAARLPPAQLQGLRRD
                     ARLWRPKTGP"
     gene            complement(10719..11060)
                     /locus_tag="PK144_07030"
     CDS             complement(10719..11060)
                     /locus_tag="PK144_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65517.1"
                     /translation="MRTRRSACTGALMLVLALGAAPGALADDAAFAALLTAASRNLQS
                     PAAPFEEEDGTPPNLDAYGFDNWLAREQPGLAREYTRLDADARRQVYARYRAEPTLAA
                     IRDSILARSRP"
     gene            complement(11057..11764)
                     /locus_tag="PK144_07035"
     CDS             complement(11057..11764)
                     /locus_tag="PK144_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNF65518.1"
                     /translation="MSSTPDPGLDERLARVLDRIRSAELRYGRTGGSVGLLAVSKTQP
                     AAAVRALAALGQSAFGENYLQEALDKRSELADLPLAWHFIGPVQSNKTRRLAEAFDWV
                     HAVDRLKIAERLSAQRPVQLPPLSVCIEVNVDGEASKSGVTPAALPELAHAVAALPNL
                     RLRGLMAIPAPAADFDAQRRPHARLRQAFEALQHDGLALDTLSIGMSDDLEAAIAEGA
                     TLVRIGTAIFGPRRAPA"
     gene            11889..>12187
                     /locus_tag="PK144_07040"
     CDS             11889..>12187
                     /locus_tag="PK144_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HNF65519.1"
                     /translation="MNIDELLAFGVKHNASDLHLSAGLPPMLRVDGDVRRINLPPMDH
                     KEVHGMIYDIMNDRQRKEYEENLETDFSFAIPGLARFRVNAFNQNRGAGAVFRTIP"
BASE COUNT         1836 a         4285 c         4359 g         1707 t
ORIGIN      
        1 actggccggc ggctgcgcgg ccgtcagcga cggcgcgagc tgcgcgagcc ccggcagcgc
       61 cgcggccatg gtctcgcccg gcagcatcgc gaagaaggcg ctggcaagct gcgtcagccc
      121 ggccacgtcc ggcaggcccg gcggcgcgag gctgccgggc agcgccgccg cggcggcggc
      181 ctcactggta ggtgtgggca tattcatgga acttgccgac ctccacgtct tcgagcacgg
      241 ccaccgcatc gtcggtcagg atcgccagcg agcagtacag cgagatcagg taggacgcga
      301 tcgcggcgcg gtcgatgccc atgaagcgca ccgacagacc cgggctctgc tcgccggtca
      361 ggccgggctg gtacaggccg atgacgccct gacggctttc gccggtgcgc agcaggagga
      421 tctgcgtcct gccgtcgacg accggaatct tgtcggacgg gatcagcggc aggccgcgcc
      481 aggtgatgaa ctggcagccg aacaggctga cggtcgccgg cggcacgccg cggcgcgtgc
      541 attcgcggcc gaacgcggcg atcgcgagcg gatgcgcgag gaagaagccc ggcgccttcc
      601 agacctttgt gatcagctcg tcgaagtcgt ccggggtcgg cgcgccattc agcgtcgaga
      661 ggcgctgcgc atcggcgacg ttggcgagca ggccgtagtc ggggttgttg atcagctccg
      721 attcctgacg ctccttgatc gtctcgatcg tcaggcgcaa ctgctccctg atctggtcgt
      781 gcggactgct gtagagatcc gacacgcgcg tgtgcacgtc gagcaccgtc gtcaccgcat
      841 tcaggaaata ctcgcgcggc tgttcctcgt aatcgacgaa ggtcttcggc agcgccgctt
      901 cgtcgcgctg cgagcaggcg acttcgacgc tggtcggatt gctgaccttg ttcagccgga
      961 agatgccggc ctcgaccggc acccattgca gcagatgcac gagccagcgc ggcgtgatcg
     1021 tcgaaagctg cggtacggtc ttggtcgcat tcgccagctg gcgcgcggca tggtcgccga
     1081 gggcgctgtg cacgtgggag ctgtcactca tcttctgaat ctccgctgtt gaaaagggcc
     1141 gctcatgcct gaacggcgat tcggacggcg accgctcatg ggtcgccgga aaatgcagga
     1201 ccggttgccg atcacaatgc tgctgtgaac cggccggatt ttttcttcct ctcccctgcc
     1261 tttctcctgc ttcgggagca aggcgctgta atgcggatgc cggatcagcg gatcgcaccg
     1321 gccgaaaacg gcgggcacga ccgggccggc gcgcgcagcc atctgctgcg cacgtgaata
     1381 tgggcgtgaa ggctccggcg gcggatcagg cggccgggtg ctgccggtgt gtcatgccga
     1441 tcacacgtga atgcggtgtt gatctccctc tcccccagcc cttccccgcg aaggggaatg
     1501 ggctgaaagg accgggtgtt cagctgtgat ggatatcagt ggttcacctg ttcctggcgc
     1561 gtgctcgcct gcgtgacgtg gctgccgggc ggcacgctgt gcgtgagcca gacgttgccg
     1621 ccgatcaccg agccacggcc gatcgtgatg cgcccgagga tggtcgcgcc ggcgtagatg
     1681 accacgtcgt cctcgaccac cgggtggcgg gccagacctt tttcgaggct gccgtcgatc
     1741 gcggtctgga aactgcgggc accgagcgtg accgcctgat acagccgcac gtggtcgccg
     1801 atcaccgcgg tttcgccgat gacgacgccg gtgccgtggt cgatgaagaa gctgcggccg
     1861 atgcgcgccc ccggatggat gtcgatgccg gtctgcgcgt gcgcgatttc ggcgacgatg
     1921 cgcgcaagca gcgccagacc ctggccgtgc agctggtggg cgagccggtg gtggatgatc
     1981 gccagcacgc ccggatagca gagcagcacc tcatcgacgc tgcgcgccgc cggatcgccg
     2041 gcaaaggcgg cctcgacatc ggcatcgagc agcgcgcgga tgccgggcag ggcttcggcg
     2101 aagcggcgca cgtgatgcac ggcctcggcc gaggccacgc cgagttcgag gcagacctgg
     2161 ccgagcagcg cgcgcagggc gcgatccagc gtgtggccga cgtagtaatc ctcgctctcg
     2221 cgctgcaggt cgtccggccc gagccgcatc ggaaacagcg cgccgcagag atcggcgaga
     2281 atgcgctcga tggcggcgcg ggccgggaat tcgcgaccgc cggccgtcag ccggcgctgg
     2341 cgcgccgtgc gccagcgttc gcgctgcgca cgtaattcgc gcacgatcgg gccgatatcc
     2401 caggactgcg cagcctccgg cgcaacaggc acctcatcgt cccggcggtt catgaaaact
     2461 ctccgtgctg cccgaatgca gcgcatggcg tttgaaacag gccggagcgg tttccggcgc
     2521 tttccggcat ccattcggcc agacggacat gccgtccgta tggcaatgat tcgtctgcct
     2581 gcccaccggc agacccgtgc ggccgaaacg ccggtgcttc acgaaaaacc ggatataacc
     2641 gcagacagcg tccagacttt ctcacttgcc catgcgcctg ccattcgtcc tctttacagg
     2701 cggggcggca gtataaggac aagccgtgca cagacaagcc ttccggcagt catccggttc
     2761 aggccagttc catgatcgca gacaggcatc cgcaataacg gacgcaatcc ggatttacgc
     2821 tgccgaatta ccggcatgtg aaatgaatgc gatcttgatg aatgcgatct tgatgaatgt
     2881 gatcttcatg aatccggaat ccagccggat caatccggat caggccgtcc gcgcgaccgg
     2941 caccggaact ctgacgccgg ctccacgact gtaaatcgga cgtgccactc acggctgcat
     3001 tccgccaccg ctcgcctcag gcgggtgcgg atgcagcctc gcgagcgttc agtcctgctc
     3061 ccagggcagg ccatggtgcc gccagccgcc ctgcccgccc cgatggccat cggcgtcgcg
     3121 ctcgccctcg aagccttcga gcacgttgta ggcgcgcgtg tagccggccg cggccagcgc
     3181 agccgcggcc gccaccgagc gcttgccgct gcgacagagc agcagcacga catcgtcctt
     3241 ctgcggcacg cgcttttcga actcgcgcag gaagcgcggg ttgcgcgtct gcgccgtgcc
     3301 ggtcgcccac ggcacgtgga ggctgccggg cacgcgcccg acatacgcga gttcctcgac
     3361 cgtgcgcacg tcgacgagca cggcgtgacc ggtggcgaac agcgtccagg catcgaccgg
     3421 cgacaggccg ccggcgtagc ccgcgggctc ggcgggacgg gcgctggcca gcacggcttc
     3481 ggcatcgatc agcgcagcgc tcagttcggg cagggcacgg gtcatgaggg gcatctcctc
     3541 ggcactcgcc gcgcctcgac cgggcgggcg ggtgtccatc acgggtcggt tgccggtcgg
     3601 acgggtgcgt ccgggcgggt gatcgatgcc ggaacgataa gccgggtttt atatttccat
     3661 aaataatgat ttatgtattt ttaatttctt ttaggtataa aaagtcccgt ctgcagccat
     3721 gcgggccgaa ccggccagcc gctttccgga atccttgtcc atcggcatct gcacgcgcgg
     3781 cagccgccgg ccccggcctc gcccggacag cgcggatggg tcaggacgac ggcgaggcat
     3841 ggatgaacaa gcccctcccc cgcgagcagg aaaaagcgga aaagccggtg tgttgcgccg
     3901 ggatcggcag gacagcggtc tttcagccgg ccggacggcc cgcggacggc aggcgccgca
     3961 gcgcgcgcgg cggagggggc agggcatcgg tcggcagcga cgctgccagc ggcagaccgg
     4021 cgagcaggcg cagcagcgcg gcctcgatgc gggtctcgac gcccggatcg agcaccagca
     4081 ggccgtcatc gtgcaggcgt gcctgccgcg ctccgatctg caggctgctc agcacgccgc
     4141 tggccccgag cgcgtcgagc accggtttca gcgcgtaatg cagcgtcagc tgccggctgg
     4201 cctccccgcc gcaggcgagc ggcagcacgc gcacaccggc cagcgccccg gcgggcagca
     4261 ggtcgagaaa ggtcttcagc acgcccgtat aggtgccgag ccgcaccggc gtcgcgatca
     4321 gcagcgcctg tgcgccggcc agcacctgca cggcggcgcg gatcgccggg gcttcgcggc
     4381 gggcgtgcag caggtcttcg gccggcagca ggccggggcg gatggcgcgc accggcaggc
     4441 cgtaatggcc gagccagtcg gcgagccagt cgagcacctg ctgcggtctg gcgtccggct
     4501 cggtacagcc ggcgagcgtc acgagatgca tgcgatgcct ctgcgcacgg cgggcgggcc
     4561 gcccgcggaa ccggcagcgt aacaagcccg cgatatgcgc aaaaagcatc aaaactcaga
     4621 tgcttattcc ataaatgaca taagactgcg ccgaagcgcc cgacccgcac ccgcttcgtt
     4681 caggcggtcg cgcgcagcgc cggcgcgatg aattcgctgg tcggcgctgc gaaatacatg
     4741 ccctgaccga gcgggatgcc gagttcgagc aggtgttcga cggtcgcacg gttctcgacg
     4801 aactcggcga tcggccggat gccgagaccg atcgccagat cggcgatggc cttgacgaag
     4861 atgcggtcgg cgcggtcggt gccgagatgg cgcacgaagc tgccgtcgat cttcacgtag
     4921 tcgacggtca ggttcttcag gtagtggaag gacgagaagc cggcgccgaa gtcatcgagc
     4981 gcgaagcggt agccggcgtt gcgcagggcc tggatgaact cgcgcgcggc ggtgaagttc
     5041 tcgaccgccg cggtctcggt gacctcgaac acgatcgcgc cgggatcggc acggtggcgg
     5101 tgcgtggcat cgcggatcag ttcgagcatg tcggcgctgc cgaaatggcg cgccgacagg
     5161 ttgatggtca ggctgaccgg gtcgttgcgc aggcggctcg cgccctgcgt ggcgatggcg
     5221 gtctcgacca cccagcggtc cagctcgcgg atcaggccga agcgctcggc cacggcgatg
     5281 aaggcattcg ggccgatggt ctcgccctgg tcaccgagca tgcgcagcag gctttcgtag
     5341 tgcacgacct tgccggtgcg caggtcgacg atcggctgga acaccagcat cagccggtga
     5401 tcgcgcagcg ccgtgcggat gcggtcctcc cagtggatct tctcgcgcat gcgcgccaga
     5461 tcgccgccgc cctcgaacac gcaccaggtg ttgcgcccgt gtgccttggc ctcgtacatc
     5521 gcggtgtcgg cgttggcgag cagctcgctg gtgtggatgc cgtgctgcgg gaagtacgcg
     5581 acgccgatgc tggccgggaa cggcgtgctg cgcccctgat ggatgaattc ctgcgcgttc
     5641 agcgtcgcga gcagcgtgcg gcagagcagt tcgctgctgt cgggatcggt gccgggcagg
     5701 atcacgccga actcgtcccc gccgaggcgc ccgagcacgt gttcgggcgc gagcggcaca
     5761 cgcaggtgct gggtgacctg gcgcaggtac tcgtcgccgg tgtggtggcc gtagctgtcg
     5821 ttgatgtact tgaactggtc gagatcgagg aacagcagcg ccccggggct ggcgccgggc
     5881 tggcgagcgg cgtcgatgtg gttctgcagc agctcctgga agcggcggcg gttgacgagg
     5941 ccggtcagcg aatcgtgatc ggccaggaag gcgacgcgct gctcggcgag cttttcctcg
     6001 gtcacgtcga gcagcaggcc ggtcaggatc ggcccttcgg cggtctgctc gcccgagccc
     6061 atcagccgca gccagcggta gctgccgtcg cggtggcgca ggcgcaggtc catcgtgtgg
     6121 ctgcgcgccg aggcgccggc gtggccgagg aaaccctgcg cccattcgcg gtcggcggcg
     6181 tggcagcagc ggtcgagaaa gtcgggcgcc agccactgcg agggcgagtg gccgagcagg
     6241 acctcggcct ccgggctgac gtagcggaag cgcccgctgc gcggctcggc ctcccagacc
     6301 acggcgctga tgccgcgcag catcgcgccg aggcgccgcg attcctgccg cagctgcatc
     6361 tgctggctgc gcgccgaatt cgcccgctcg ccgagccgcg ccgccagcag gttcacggtc
     6421 tcgccgatgc gcgcgagatc cccggccggc agcgccggca ggcgcagctc gtagtcctcg
     6481 cgcgcgagcg catcgagacc gcggcgcacg tgaccgaggc cgccgcgcag ctcgctgccg
     6541 aggcgcagca gcgcgaagcc ggtcagcacg agcccgccga gcacgatgcc ggcgttctgc
     6601 agcagcagcg tgttgtcctg cccgtaggcc gagggatcga ggccgatcca cacgaaaccg
     6661 gccagccggc cgctggtgcc ggcgaccacc ggcagttcgc tgcgcagcac gccatcgacg
     6721 ctgctcgtgc ggtattcgcc gggcggcggc acggccggat cggcccccgg tgcgtcaccg
     6781 atgcgcgcga gcacccggcg ctcgggcgtg aacaccgccg cgtaacgcag cgccggatcg
     6841 gcgctcagcg gtgcgagcgc ggcctgcgcg gcctcggcgt tgcgctgcgc cagcgccggc
     6901 tccagggccc gcgccagcag gccggcgcgc tcgcgaccgg cgagcgcgtg caaccgggtt
     6961 tcgagctgcg actgcacgcg ccaggtgttg ccgagcacgg cggcgagcgt gagggcggca
     7021 gcggcggtgg ccgcgatcag caggacggtc agcgggcgca gcggcatcgg atcgggactc
     7081 cttgacgagc gccggccggc ggcgcttcct gaaacgggcg gctgcgctca ggcgccggcg
     7141 gccggcgcac acaggccggc ggcgagcagc acctcgggca gccggcgcgg ggcgcggatg
     7201 cgcaggcgct tgacgcgcgc ggcatcgccg gcgagcagtt cgacggcgga cttcggcacg
     7261 ccgaagctgt cggcgagcag cgcgcagagc cgggcgttgg cctggccgtc gaccggcgct
     7321 gcgcacagac gcacgcgcag gcagccgtcg cgcacctctt cgagccggtc gcggctcgcg
     7381 cgcggctgca cgcgcacgcc gagcacgaga tcggccccct cccagcgcca gcaggcggct
     7441 tccgggctca tgcgctgatg cggaccttca gtgcagcgcc agcgcgaggt cgcgcagcgg
     7501 agcgatcagc aggatgctgg cgagctgcag cacgacgatc accagcagcg gcgacaggtc
     7561 gacgccaccg accagcggca gcacgcgctg cgccggacgc agcaccggcg cggtcagccg
     7621 gtacagcagg ccatagagcg gattgcgcgg atcgcgcacg aaccagctca tcagcgcctg
     7681 gatgatcacg gcccacagga acacgtcgag cgtcagaccg agcagttcga cgagtccgag
     7741 cagcagcagc ccgagcggcc cgaagctgcg cccgaccatc gacaggatca ggccccattc
     7801 gaggatcgcg agcagcagca tcagcagcac gcaggccatg tcgagcccgt acaggcccgg
     7861 cacgtagcgg cgcagcggca gcagcggcgg gttggtgatg cgcacgagga actgcacgag
     7921 cgggttgtag aaatcggcgc gcacccactg cagcaggaac cgcagcatca gcgccagcag
     7981 gtagaagccg aacacggtgc ggatcaggaa cacgcccgct tcgctgaaat agggcgtccc
     8041 gcccatcatg tcccctcccc gccaccggcg gccggcgtat cggcaaacag gtccccgagc
     8101 tcacgcgaac gctgcgcggc ggcggcgagc gcctcggcga agatcgcttc gatcccggcg
     8161 ttcttcagca cctgcagcgc ggcttcggtc gtgcccttcg gcgaggtcac gcgcgcgcgc
     8221 agttcggccg ggctctcgct cgattcgagc gcgatgcgcg cggcgccgaa ggcggtctgc
     8281 agcgtcagca ggcgcgcggt gtcggccggc aggccgagcg tgcgcgccgc ggcttccatg
     8341 gcctccatga ccaggaagaa gtacgccggc ccgctgcccg agagcgcggt gacggcgtcg
     8401 agccgggcct cgtcatcgac ccagacggtc agcccgaccg cgcgcaggat cgattcggcc
     8461 agccggcgct gctcgacgct gacgccggcc gcggcgtaca gcgcggtcgc gccgcttttc
     8521 acgagcgccg gcgtgttcgg catcgtgcgc accagcgcgc cgtgaccgcc gagccagcgg
     8581 ccgatgtcgg cgagccgcac gccggcggcg atcgacacga ccagcggccg ggcgtgctgc
     8641 acggcctcgg cgatgccgag ggcgacctcg cgcaggatct gcggcttgac ggcgagcacg
     8701 acgacgccgg cgccgtcgat cacgtcgggg ttgtgctcgc tggtctgcac gccgtactgc
     8761 gcgcgcagcg cctcgcgacc gccggccgag ggttcggcca cacgcagcag ggcggccgga
     8821 cagccgtcgg cgagcaggcc gccgatcagg ctgcgggcca tgttgccgcc gccgatgaag
     8881 gcgatggggg tgcggatcat gcagcaaggc ctcgggcgtc gtacgggaac cgcgcaaagc
     8941 taagcgcggt ccggcgaatc cgcaaccgcg cgccgcccgg ccgcgcctgc ttcaggggcc
     9001 ggtcttcggg cgccagagcc gcgcatcgcg ccgcagcccc tgcagttgcg ccggcggcag
     9061 ccgcgcggcc agcgtctcgc gcgcctgcag cgccacctga tcgccgtcga gggcggccag
     9121 atccagccat ttccacgccg ccaccggatc ggcgggcatg ccgcggccgt cgcgcagcag
     9181 caggccgtag gcggtctgcg ccggcgcata gccctgctcg gccgctgcgc gcagcagaac
     9241 ggcggcctgc gcctcgtcgc gctgcacgcc ccgcccggtc agcgacgcca tgccgagcag
     9301 gaagcgcccg ggcgcgtagc cggcgcgcgc ggcccgcgca taccagcgcg ccgcctgctc
     9361 gtcatcgcgc gccacgccgc tgccttcggc gtaggcaagg ccgagatcga cctgcgccag
     9421 cccgagcccc tgatcggccg cccggcgcag ccacagcacg gccagttcgg gatcgcgctc
     9481 ggtgccggtg ccggcgcgcg tcagcagcgc cagcgcgagc tgcgccggcg catgaccggc
     9541 ctcggccgcg gcccggtatt cgcgcacggc cagcagcaga tcggcggcga tgccgcgccc
     9601 gcgctggtag tgctcgccga gccgggtatg ggcgctggca agcccctgct tggcggcacg
     9661 tgcgtaccag accacggcct cggcatcgtc gcgcggcacg ccggcgccct tcgccagccg
     9721 ctcggccagc tcgaactgcg cgcgcggatg gccggcctcg gccgccccgc ggtagagctg
     9781 cacggcgcgg cgctcgtcgc gggccacgcc ctcgccgttt gcatacagcc cggccagcgt
     9841 gaactgcgcc agcggctcgc gcgcctcggc cgcggccccg agccagcgca cggccagcgc
     9901 cagattctgc gtgccggtca gcccctgcag atggatcagg ccgagcgaca gctgcgccgg
     9961 cggaaagccg ctttcggcgg cgcgcaccag gccgggcatc acctcggcca gctccagccc
    10021 cggcacccgg ccgctggctg cgagttcggc gagctgctgc gcggcgccgg gctggccgcg
    10081 ggtcgcggcg cggcggaacc attcgaccgc cgtgcgcgga tcaccgagca cgccctcgcc
    10141 acgcagatag cagagaccga gccggtattc ggcatcggca tcgccggcct cggcaccgcg
    10201 catcagccac tgcaggcctt gggcgacatc gcgcgcgacg ccctggccat cgaccagcgc
    10261 cgtgccgtag cggccctgcg cgcccgcatg cccgccctcg gccgcctgcc ggtaccagcg
    10321 caccgcgccg ggcaggtcga gcgcgccgcc gcggccggtc gcgagcagat ccccgagccg
    10381 gaagcgggcc tcggcatagc cgttgccggc ggcgatgccg aacagctcgc gcgccttcgc
    10441 ctcgttgcgc gacaccccgc ggccttcgag gtacagctcg ccgagcttgg tctgggcgcg
    10501 ggcgtcgccc atcttcgccc aggtttcgta ttccttcgcc gccgcggcgt cctggccggt
    10561 ttcgagcagc gcatcggccc gggccccggc ctgcggcggc cggtcgccgt agtgccagcg
    10621 gccctgttcg tcctgccatt tcatcatcgg cggcgcagcc gcggccggca gcgccggacc
    10681 gagtgcgacg atcagggcca gcgccatcat gcgcacgctc atggccggga ccgcgcgagg
    10741 atcgaatcgc ggatcgccgc gagcgtcggc tcggcccgat agcgcgcata gacctgccgg
    10801 cgggcatcgg catcgagccg ggtgtactcg cgcgccagac cgggctgctc gcgggccagc
    10861 cagttgtcga agccgtaggc atcgaggttg ggcggcgtgc cgtcctcctc ctcgaagggc
    10921 gcagccggcg actgcaggtt gcggcttgcg gccgtgagca gcgcagcgaa cgcggcgtca
    10981 tcggccagcg ccccgggcgc ggcgccgagc gcgagcacca gcatcagcgc gcccgtgcag
    11041 gcagagcgcc gcgtcctcat gccggcgccc tccgcggccc gaagatcgcc gtgccgatgc
    11101 gcaccagcgt cgcgccctcg gcgatcgcgg cttcgagatc atcggacatg ccgatcgaca
    11161 gcgtgtcgag cgcgagcccg tcgtgctgca gcgcctcgaa agcctgacgc aggcgcgcgt
    11221 gcggccggcg ctgcgcatcg aaatccgccg ccggcgccgg gatcgccatc agtccgcgca
    11281 ggcgaaggtt cggcagcgcc gcgaccgcgt gcgcaagctc cggcagtgcg gccggcgtca
    11341 ccccgctctt gctggcttcg ccgtcgacgt tgacctcgat gcacaccgac agcggcggca
    11401 gctgcaccgg tcgctgcgcc gacaggcgct cggcgatctt caggcgatcg actgcatgca
    11461 cccagtcgaa ggcttcggcg agccggcggg tcttgttcga ctgcaccggc ccgatgaaat
    11521 gccaggcgag cggcaggtcg gcgagctcgc tgcgcttgtc cagcgcttcc tgcaggtagt
    11581 tctcgccgaa ggcgctctgg ccgagtgcgg cgagtgcgcg cacggccgcg gccggctggg
    11641 tcttgctgac ggcgagcagc ccgacgctgc cccccgtgcg gccgtagcgc agctcggcgc
    11701 tgcggatgcg gtcgaggacg cgcgcgaggc gctcgtccag gcccggatcg ggggtactgc
    11761 tcatgcgggg actccggtcg gccgtggctc ggctctgctt gctatcggct tgctatagtg
    11821 cggcgcactt cccgtatcca gtcacacccg gtcacgcaca acgtcacgta cacacggagc
    11881 ggccgcgcat gaacatcgac gaactgctgg ccttcggggt caagcacaac gcctcggacc
    11941 tgcacctgtc cgcgggcctg ccgccgatgc tgcgtgtcga tggtgacgtg cggcgcatca
    12001 acctgccgcc gatggaccac aaggaagtgc acgggatgat ctacgacatc atgaacgacc
    12061 gccagcgcaa ggagtacgag gagaacctcg aaaccgactt ctccttcgcg atccccggcc
    12121 tcgcgcgctt ccgcgtcaac gccttcaacc agaaccgcgg cgccggcgcg gtgttccgca
    12181 ccatccc
//
LOCUS       DAOQIE010000084        12183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2553_length_12183_cov_8.056728, whole genome shotgun sequence.
ACCESSION   DAOQIE010000084 DAOQIE010000000
VERSION     DAOQIE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12183
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_12183_cov_8.056728"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PK144_07045"
     CDS             <1..715
                     /locus_tag="PK144_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF65520.1"
                     /translation="LRRSQNWIGPAGCTLATASFVPPPHEEVPRILGELEKFLHQEDE
                     LPPLVKIALAHVQFETIHPFLDGNGRVGRLLITFLLTERGVLHKPVLYLSHYFRRHRQ
                     AYYEHLQAVRDRGEWERWLAFFLRGVSEVAGEATETARRIQLLREQHRSAITDRLGRA
                     AGNGHRVLESLFNRPIVTVADVQTLTGTTYAAANTLVARLAGLGVLAEMTGYARNRRF
                     RYEPYVRLFTDEPLPEHPT"
     gene            1063..>1799
                     /locus_tag="PK144_07050"
     CDS             1063..>1799
                     /locus_tag="PK144_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNF65521.1"
                     /translation="MSADDEIVNPSHGPRFTDLLALAASRRQLLKLGAAGAALALFGR
                     SGAAADAPAAAAAPAAALAGLPGFTAIATSTEDTVRVPPGYEWAVLTRWGDPVGAQAG
                     QPRWRSDAGNDAAEQALQFGMHHDGMALFPLGTDGTRALLALNHEYVDHGLLFADGMD
                     GWSAAKVAKSMAAHGVSVVELVQADGRWSVVSPSQYARRITAATPMRLAGPAAGHALL
                     ATAADPQAQTVLGTFNNCACGQTPWGT"
     assembly_gap    1800..1899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1900..3185
                     /locus_tag="PK144_07055"
     CDS             <1900..3185
                     /locus_tag="PK144_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNF65522.1"
                     /translation="VLGTFNNCACGQTPWGTYLSCEENFNGYFARTAGEPTALEKRYG
                     IREKSDYRWHEHEPRFDSALHPNEAHRFGWVVEIDPHDPAAVPVKRTALGRFKHENAA
                     VTLAADGRVVVYLGDDQANEYLYRYVSKGRYAPGMARAAAGALLDDGTLSVAHFGADG
                     HGEWRALVHGRDGLDAGNGFADQAEVLVKTRLAADVRGATKLDRTEWVAIHPGRAEVA
                     VTLTNNSGRKSADAADPRAPNPHGHILRWRPDGADHAADGFAWEVFVLAGDPQAEDAS
                     ARGNQPGLNAANTDAAFSSPDGLAFDARGLLWIETDVSTGKLWHPQLAPKQGAYQVFG
                     NNQLLVANPDTGEIRRFLTGPVGCELTGIAFSPDLRTLFVNIQHPGEPLDANTEFNDP
                     TAPQRWSQWPDGPGGGRPRSATLAIRRTDGGPVGS"
     gene            complement(3544..4371)
                     /locus_tag="PK144_07060"
     CDS             complement(3544..4371)
                     /locus_tag="PK144_07060"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF65523.1"
                     /translation="MRERSFRSVWLSDLHLGNADCRALWLLDFLDACTCEQLYLVGDV
                     LDLWEMDRRGRWPATHGRVLAKLIALAAAGTRVVWLPGNHDEALRAHHGLRFGGVELL
                     PEAIHETADGRKLWVVHGDGFDDAARGGRLTTLIGDHAYDLALFLNRWSRRLRRLCGL
                     PYRSLALELKTRIGPAARAIGRYERAAAHEAARREVDGIVCGHIHKPELRTIDGTLYA
                     NCGDWIESCTALVEHADGRLQVLHWADEARVTHVDVLDFPRAAAAVPAAAAMAGRLG"
     gene            complement(4660..4857)
                     /locus_tag="PK144_07065"
     CDS             complement(4660..4857)
                     /locus_tag="PK144_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65524.1"
                     /translation="MQHTLESRDSIRRLANGTIDYDWYIARADALRHEALHGQRPVGY
                     SATGRGSALPVFGAHGAAHGV"
     gene            4938..5891
                     /locus_tag="PK144_07070"
     CDS             4938..5891
                     /locus_tag="PK144_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF65525.1"
                     /translation="MRELNSSVQPAATHRLPSLIALRAFEAAARLGSFQAAAAELHVT
                     PSAISHQIRALEGELGVPLFERLDKRVVLARGGQELFPAVREAMELLQAASLRVRRLA
                     GERRLVLSSAPLFATRWLMPRLADFHARHPDWELHVRATLEEEDFRSGEIDLAIRWGD
                     RDRHWPQHLAHEELLIERITAVASPDLAARLAPGSGPGALIDGAPLLASAFRDAEWQR
                     WFDAAGCLKPAPLPSAHFASTAHMLEAAVAGMGWALVDPRFAGRELAQGLLVAPWPLA
                     GCEGQHFHAVCPPAHTGRPGVARFVSWVRERLAAEAPATAG"
     gene            5997..7067
                     /locus_tag="PK144_07075"
     CDS             5997..7067
                     /locus_tag="PK144_07075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNF65526.1"
                     /translation="MTRAQIENVNVVSTEMLLTPAELHKRLPLTADARAAVVAGRETV
                     EAILDRRDPRLFVVVGPCSIHDPAAAMDYARRLKALSDELGDTLYLIMRVYFEKPRTT
                     TGWKGLINDPDMDDSFKIQKGLGVARQLLTDIAELGLPTGTEALDPISPQYLSDLITW
                     AAIGARTTESQTHREMASGLSTPVGFKNGTDGGLAVAINALLSVSKPHSFLGIDGEGQ
                     CSIIRTRGNRYGHVVLRGGPKPNYDSVTIALCEQELAKNKLPANIVVDCSHANSSKKH
                     ELQPLVLNDVVNQIVDGNRSILGVMIESNLNAGNQPIPADLSQLKYGVSVTDACVDWE
                     TTERMLRDAHAKLKDVLPKRLG"
     gene            complement(7221..8984)
                     /gene="icd"
                     /locus_tag="PK144_07080"
     CDS             complement(7221..8984)
                     /gene="icd"
                     /locus_tag="PK144_07080"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNF65527.1"
                     /translation="MAYQHIKMPATGEKITVVDGKLSVPDQPVVGYVEGDGIGPDITR
                     ACLRVWDAAVEQAYDGKRKIHWCELFMGEKAAEIYDGNYFPDETLEALKDLIVSIKGP
                     LTTPVGGGFRSLNVALRQELDLYACVRPVRYYAGVPSPMVHPELVDIVIFRENTEDVY
                     SGIEYKAGTPENEKVAKFLREEMGAEFFEGAGLGIKPVSAFGTKRLVRKAIRYAIDHK
                     KDSVTLVHKGNIMKFTEGAFRNWGYEVARDEFGDVTITEDELYSVYGGVQPEGKIVIK
                     DRIADIIFQLLQLRPAEFSVLATLNLNGDYLSDAAAAHVGGVGIAPGANIADYVAVFE
                     ATHGTAPKYANLDKVNPGSLLLSGVMMLEYMGWQEAADLINSALTKVIADKTVTYDFA
                     RQMPGATEVKTSQFGDLLIAKIRGETAALVAAAEAHRAQVEAEKSKLEEQRQANPAEA
                     MKEAGRQPHAVADIMHKVVTVKGTESVAEVMHMMRESRISSVVVEPDDKGEWGIMTQR
                     DIVRKIINANRSPARVVVNEIVNRPLVMVSRDTTLTEASSKMLEANIRRVVVGASGTP
                     IGVVSDMDLFRTVEEYGWGGE"
     gene            9557..9859
                     /locus_tag="PK144_07085"
     CDS             9557..9859
                     /locus_tag="PK144_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65528.1"
                     /translation="MTMRTSIPGIVAGLALTGMAAAAVAADGAWIPRGKPMRQFPSCY
                     QMWVCVAKDKPEGPFSGMPRGTWGACDTSYTAGAAGSQAESCEKCLAGPPEDACKP"
     gene            9923..10870
                     /locus_tag="PK144_07090"
     CDS             9923..10870
                     /locus_tag="PK144_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNF65529.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAVRKLEDELG
                     VVLFERGPGEVTVTPVGRRVVGQAQRVLEEADAIRALALSGKDELAGVLRLGAIYTIG
                     PYLLPQLIPRLKRVAPHMPLQIEENYTAVLGERLKQGKLDVLILSLPFEESGVLTEAL
                     YEEPFVVVMPAGHALEHERAIDVHALAQEDLLLLGPGHCFRDQVLQVCPECNRAAVAS
                     DADNMQRTLEGSSLETIRLMVATGMGVTVLPQTSMTAYASAPELLSVRPFTEPAPKRV
                     VALAWRKSFPRPAAIRVLAAAVRACPLGESIEVLEGATA"
     gene            complement(10921..11187)
                     /locus_tag="PK144_07095"
     CDS             complement(10921..11187)
                     /locus_tag="PK144_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65530.1"
                     /translation="MTEKDRFEAATEMVATAIQTAGVFGENQRITRLIVGNLGRMAAE
                     LDAEPGSPGGRALIRHALAGIDAAEAALVPKLIEGLQALDRDPG"
     gene            complement(11235..11969)
                     /locus_tag="PK144_07100"
     CDS             complement(11235..11969)
                     /locus_tag="PK144_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNF65531.1"
                     /translation="MSESEVEPITQEAALRVALAARAMPNIALAQLVQVLHNRLGDHI
                     DTDKLRTVTVTDLKTGLGSVDGEEDNEDILIGLDAMKMAVRILWGDDDGTADLPPVES
                     YAEGDMPGSVRIALASDSGTTLNGHFGSCLRFLVYQVSPTESRLVGIRDALEADFAED
                     KNAFRTSLISDCHVLYVVSIGGPAAPKVIRADIYPIKRPEGGEAAEVIAQFQQAMIKS
                     PPPWLSKILGVAAGQRVKNYDAGTRA"
BASE COUNT         1839 a         4218 c         4237 g         1789 t
ORIGIN      
        1 gctgcgtcgc agtcaaaact ggatcggtcc ggcaggttgc actctggcga ccgccagttt
       61 cgtgccgcct ccgcacgagg aagtgccgcg aatcctcggg gagctggaaa agttcctgca
      121 tcaggaggat gagctgccgc cgctggtgaa gatcgcactg gcgcatgtgc agtttgaaac
      181 cattcaccct ttcctcgacg gcaacggccg cgtgggccgg ctgctgatca ccttcctgct
      241 gaccgaacgc ggcgtgctgc acaaacccgt gctctacctg tcgcactatt tccgccggca
      301 tcgacaggct tactacgaac acctgcaggc ggtgcgcgac cgtggcgaat gggaaaggtg
      361 gctggctttc ttcctgcgag gcgtgagcga ggtggcaggg gaagcgaccg agacggcacg
      421 ccgcatccag ctgttgcggg agcagcatcg cagcgcgatc actgaccggc ttggccgtgc
      481 cgcaggcaat ggacaccggg tactggaaag cctgttcaac cgccccatcg tgacggtggc
      541 agacgtgcag acactgactg gaaccacgta tgcggcggcc aacaccctgg tggcgcgtct
      601 ggccggactg ggcgtgctgg cggaaatgac gggttatgca cgcaaccgca gattccgcta
      661 cgagccctat gtccggctgt tcacggatga gccgcttcca gaacacccga catgaacctg
      721 caccgcgaac acctcttcgc agccggaagc tgacctcctc gtccacggcc ggcagtacgt
      781 cgaaggcgag gcggcccgaa aagcgttcgc gcacttcgca agaaccacca cgacccaggc
      841 ccgagcctgc accggcgcac ttcgcagtcc gccagcacgg agaggctgcc cggatcattg
      901 cggccgggaa gtcctgcacg cccccgggac gatgatgtcg cggcttcacg cacctgagcg
      961 ccgtggagtg aagtgcactg caaacccccc gtcacacagc ccgcgcacac tcgcacgcct
     1021 tccgcggccc gagccggccg tttcgcgatc gaggtagtgc cgatgtccgc cgacgacgaa
     1081 atcgtcaatc ccagccacgg cccgcggttc accgatctgc tggcgctggc cgccagccgc
     1141 cggcagctgc tgaagctcgg tgccgccggt gcggcgctgg cgctgttcgg gcgcagcggt
     1201 gccgctgccg atgcgccggc ggccgctgcg gccccggctg ccgcgctcgc cggcctgccc
     1261 ggtttcacgg ccatcgccac cagcaccgag gacacggtgc gcgtgccgcc gggttacgaa
     1321 tgggcggtgc tgacgcgctg gggcgatccg gtcggcgcgc aggccgggca gccgcgctgg
     1381 cgcagcgatg ccggcaatga tgccgccgag caggcgctgc agttcggcat gcatcacgac
     1441 ggcatggcgc tgttcccgct cggcacggac ggcacgcgcg cgctgctggc gctgaatcac
     1501 gaatacgtcg atcacggcct gctgttcgcc gacggcatgg acggctggag cgccgccaag
     1561 gtcgcgaagt cgatggccgc gcacggcgtc agcgtcgtcg agctggtgca ggccgatggc
     1621 cgctggagcg tggtctcgcc gtcgcagtac gcgcggcgca tcacggccgc gacgccgatg
     1681 cgcctcgccg ggccggccgc cgggcatgcg ctgctggcga ccgccgccga tccgcaggcg
     1741 cagacggtgc tcggcacctt caacaactgc gcctgcggcc agacgccgtg gggcacgtan
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtgctcggc accttcaaca
     1921 actgcgcctg cggccagacg ccgtggggca cgtacctgag ctgcgaagag aacttcaacg
     1981 gctacttcgc gcgcacggcc ggtgaaccga cggcgctcga aaagcgctac ggcatccggg
     2041 agaagtccga ctaccgctgg cacgagcacg agccgcgctt cgactccgcg ctgcacccga
     2101 acgaggcgca ccgcttcggc tgggtggtcg agatcgatcc gcacgatccg gcggcggtgc
     2161 cggtcaagcg caccgcgctc gggcgcttca agcacgagaa cgccgcggtg acgctggccg
     2221 ccgatggccg cgtcgtcgtc tatctcggcg acgatcaggc caatgaatac ctgtaccgct
     2281 acgtcagcaa gggccgctac gcgccgggca tggcgcgcgc cgcggccggg gcgctgctcg
     2341 atgacggcac gctgtccgtc gcgcacttcg gcgccgatgg tcacggcgaa tggcgggcgc
     2401 tggtgcatgg ccgcgacggc ctcgatgccg gcaacggctt cgccgatcag gccgaggtgc
     2461 tggtcaagac ccggctcgcg gccgatgtgc gcggcgcgac gaagctcgat cgcaccgagt
     2521 gggtcgccat ccacccgggc cgcgccgagg tcgcggtgac gctgaccaac aacagcggcc
     2581 gcaagagcgc cgatgccgcc gatccgcgcg cgccgaaccc gcacggccac atcctgcgct
     2641 ggcgcccgga tggcgccgat cacgccgccg acggcttcgc gtgggaggtg ttcgtgctcg
     2701 ccggcgatcc gcaggccgag gatgccagtg cccgcggcaa tcagcccgga ctcaacgccg
     2761 ccaacaccga tgccgccttc agctcgccgg acggcctcgc cttcgatgcg cgcggcctgc
     2821 tgtggatcga gaccgatgtc tcgaccggca agctctggca tccgcagctc gcaccgaagc
     2881 agggcgcgta tcaggtcttc ggcaacaacc agctgctggt cgccaatccg gacaccggcg
     2941 agatccgccg cttcctgacc ggcccggtcg gctgcgagct gaccggcatc gccttcagcc
     3001 cggatttgcg cacgctgttc gtcaacatcc agcaccccgg cgagccgctg gacgcgaaca
     3061 ccgagttcaa cgacccgacc gcgccgcagc gctggagcca gtggccggac ggccccggcg
     3121 gcggccggcc gcgctcggcg acgctggcga tccggcgcac ggatggcggg ccggtcggca
     3181 gctgagcgcg tgaagcggac gtgtgcgcga ccggatgcgt cgcgcacacg gttgcggaca
     3241 cgcagccgca aggcccgaag ccgcacgtgc aaggctttgc gggcgatccg caagggaaaa
     3301 agccttgcgc gcaaggctgg aggcgtgaag cgcaaggccc gaagcagcac gcgcaaggct
     3361 ttgcgcgcga tccgcaaggg aaaaagcctt gcgcacaagg ctggaagcgc gaagcgcaag
     3421 gctcgaagcc gtgcgtgcaa ggcttcggac ccgaagtgca aggctcgaag ccttgcacct
     3481 gcggcggcca ggctttcggc tgcgggaaaa agccgtgcag gccagacgcc gggccggccg
     3541 gtttcagccc aggcggcccg ccatcgccgc ggctgccggg acggcagcgg ccgcgcgcgg
     3601 gaagtcgagg acatcgacgt gcgtgacgcg cgcctcgtcg gcccagtgca gcacctgcag
     3661 ccggccatcg gcgtgctcga ccagcgccgt gcagctctcg atccagtcgc cgcagttggc
     3721 gtacagcgtg ccgtcgatcg tgcgcagctc gggcttgtgg atgtggccgc agacgatgcc
     3781 gtcgacctcg cggcgcgcgg cctcgtgcgc ggcggcgcgc tcgtagcggc cgatggcgcg
     3841 ggcggccggg ccgatgcgcg tcttcagctc cagtgccagc gagcggtacg gcaggccgca
     3901 gagccggcgc agccgccggc tccagcggtt caggaacagc gcgagatcgt aggcgtgatc
     3961 gccgatcagc gtcgtcagcc ggccgccgcg cgcggcatcg tcgaagccgt cgccgtgcac
     4021 gacccatagc tttcggccgt cggcggtctc gtggatcgcc tccggcagca gctcgacgcc
     4081 gccgaagcgc aggccgtgat gcgcccgcag ggcctcgtcg tgattgccgg gcagccagac
     4141 cacgcgcgtg ccggcggcag cgagcgcgat cagcttcgcg agcacgcgcc cgtgcgtggc
     4201 cggccagcgc ccgcggcggt ccatctccca cagatcgagc acgtcgccga ccagatagag
     4261 ctgctcgcag gtgcaggcat cgaggaaatc gagcagccag agcgcacggc agtcggcatt
     4321 gccgaggtgc aggtccgaaa gccacacgga ccggaaggat cgctcgcgca tcatcgtcat
     4381 tcgttcaccc gctcgctccg gcccggaagg tcggctgagt ctgggcagcg gcgatgaagc
     4441 ggccgtgacg ttcgcacggc gaagcggtgg cgggatcgtg atgggccggt gacggcgggc
     4501 gtggcgatca gcggcagacc ggtagccggg cgcgcgaagg gacacgccgc ccggccggga
     4561 ccggagttgc cggtccggcc ggacggcgcg ggcagggtcg ggacggggtg gatgacatcg
     4621 gttcgggtgc cgggcgggag cggacaggcc gcgcagggtt cagacgccgt gcgcggcgcc
     4681 gtgggcaccg aacaccggca gggcgctgcc gcggccggtc gcggagtaac caaccggacg
     4741 ctggccgtgc agggcctcgt ggcgcagcgc gtcggcgcgg gcgatgtacc agtcgtagtc
     4801 gatcgtgccg ttggcgaggc ggcggatgct gtcgcgggat tcaagcgtat gctgcatggc
     4861 ggcatctcct gacaggttgg atgacgcccg caggatcatg gacttgccac acccgcgaca
     4921 aacgacgaat tttcccgatg cgtgaactga attcatctgt gcagccggcc gcgacgcacc
     4981 ggctgccgtc gctgatcgcg ctgcgcgcct tcgaggccgc cgcgcgcctc ggcagcttcc
     5041 aggccgcggc ggcggaactg catgtgacgc cctcggcgat cagccaccag atccgcgcgc
     5101 tcgaaggcga actcggcgtg ccgctgttcg agcgcctcga caagcgcgtc gtgctggcgc
     5161 gcggcgggca ggagctgttt ccggccgtgc gcgaggcgat ggagctgctg caggcggcga
     5221 gcctgcgcgt gcgccggctc gccggcgagc ggcggctggt gctgtcgagc gcaccgctgt
     5281 tcgcgacgcg ctggctgatg ccgcgcctcg ccgacttcca cgcccgccac ccggactggg
     5341 agctgcacgt gcgcgcgacg ctggaagagg aggacttccg cagcggcgag atcgatctgg
     5401 cgatccgctg gggcgaccgc gaccggcact ggccgcagca cctcgcgcac gaggagctgc
     5461 tgatcgaacg catcaccgcg gtcgcaagcc ccgatctggc cgcgcggctc gctccgggca
     5521 gcggtcccgg cgcgctgatc gacggcgccc cgctgcttgc cagcgccttt cgcgacgccg
     5581 aatggcagcg ctggttcgat gccgccggct gcctgaagcc ggcgccgctg ccgagtgcgc
     5641 acttcgcgag caccgcacac atgctcgaag ccgcggtcgc cggcatgggc tgggcgctgg
     5701 tcgatccgcg cttcgccggg cgcgagctgg cgcagggcct gctggtggcg ccgtggccgc
     5761 tcgccggctg cgagggtcag cacttccacg ccgtctgccc gccggcgcac accggccgcc
     5821 cgggcgtggc gcgcttcgtg agctgggtgc gcgagcgcct ggcggccgag gctccggcaa
     5881 ctgccggttg acgcctgccg cgcacgcgcg ctaaaccgcg catcccgttg cgtatcgcac
     5941 gcctaaccgg ctgtccgcag ccgttttctc ctcccccctt ccagggaagt gcggccatga
     6001 ccagagccca gatcgaaaac gtcaacgtcg tctcgaccga gatgctgctg acccccgcgg
     6061 aactgcacaa gcgcctgccg ctgactgccg acgcccgcgc cgccgtcgtc gccggtcgcg
     6121 agaccgtcga ggccatcctc gaccgccgtg acccgcgcct gttcgtcgtc gtcggcccgt
     6181 gctcgatcca cgacccggcg gcggcgatgg actacgcgcg gcgcctgaag gcgctgtccg
     6241 acgaactcgg cgacacgctg tacctgatca tgcgcgtgta cttcgagaag ccgcgcacga
     6301 ccaccggctg gaagggcctg atcaacgacc cggacatgga tgattccttc aagatccaga
     6361 agggcctcgg cgtcgcccgg cagctgctga ccgacatcgc cgaactcggc ctgccgaccg
     6421 gcaccgaggc gctcgacccg atcagcccgc agtacctgtc ggacctgatc acctgggcgg
     6481 cgatcggcgc gcgcacgacc gaatcgcaga cgcaccgcga gatggcctcg gggctgtcga
     6541 cgccggtcgg cttcaagaac ggcaccgacg gcggactggc ggtggcgatc aacgcgctgc
     6601 tgtcggtgtc gaagccgcac agttttctcg gcatcgacgg cgagggtcag tgctcgatca
     6661 tccgcacgcg cggcaaccgc tacggccacg tcgtgctgcg cggcggtccc aagccgaact
     6721 acgactcggt caccattgcc ctctgcgagc aggaactggc gaagaacaaa ctgccggcca
     6781 acatcgtcgt cgactgcagc cacgccaact ccagcaagaa gcacgaactg cagccgctgg
     6841 tgctgaacga tgtcgtcaac cagatcgtcg acggcaaccg ctcgatcctc ggcgtcatga
     6901 tcgaaagcaa cctcaacgcc ggcaaccagc cgatcccggc cgatctctcg cagctcaaat
     6961 acggcgtctc ggtcaccgac gcctgcgtcg actgggagac caccgagcgc atgctgcgcg
     7021 acgcgcacgc gaagctgaag gacgtgctgc cgaagcgcct cggctgaaac cggcagcccc
     7081 gccgccgccc gcaggccccg ccccgtgcgg ggccttgcat tttcaggccc ggcagaaatg
     7141 aagacggcgc cggcggcccc cttgcgggga accgccggcg ccgtccgtac ggaaggcgcc
     7201 gcgcagccgc ggcccggggc ttactcgccg ccccagccgt attcctcgac cgtgcggaac
     7261 aggtccatgt cggagacgac gccgatcggc gtgccgctcg cgccgaccac gacgcggcgg
     7321 atgttggctt cgagcatctt cgacgaggct tcggtcagcg tggtgtcacg cgagaccatc
     7381 accagcggcc ggttgacgat ctcgttgacg acgacgcggg ccggcgagcg gttggcgttg
     7441 atgatcttcc gcacgatgtc gcgctgggtc atgatgcccc attcgccctt gtcatccggc
     7501 tcgaccacga ccgagctgat gcgcgattca cgcatcatgt gcatgacctc ggcgaccgac
     7561 tcggtgccct tgacggtgac gaccttgtgc atgatgtcgg caacggcatg cggctggcgg
     7621 ccggcttcct tcatcgcctc ggccgggttg gcctgacgct gctcctcgag cttggacttc
     7681 tcggcctcga cctgggcgcg gtgcgcctcg gcggcggcga ccagcgcggc cgtctcgccg
     7741 cggatcttcg cgatcagcag gtcgccgaac tggctggtct tgacctcggt ggcgcccggc
     7801 atctgacgcg cgaagtcgta ggtgaccgtc ttgtcggcga tgaccttggt cagcgccgag
     7861 ttgatcaggt ccgcagcctc ctgccagccc atgtattcga gcatcatcac gccggacagc
     7921 agcagcgagc ccgggttgac cttgtcgagg ttggcgtact tcggcgccgt gccgtgcgtg
     7981 gcctcgaaca ccgcgacgta gtcggcgatg ttggcgcccg gggcgatgcc gacgccgccg
     8041 acgtgcgcgg cggccgcgtc ggagaggtag tcaccgttga ggttcagcgt cgccagcacc
     8101 gagaactcgg ccgggcgcag ctgcagcagc tggaagatga tgtcggcgat gcggtccttg
     8161 atgacgatct tgccttccgg ctgcacgcca ccgtagacgc tgtacagctc gtcctcggtg
     8221 atcgtcacgt cgccgaactc gtcgcgcgcg acctcatagc cccagttgcg gaacgcaccc
     8281 tcggtgaact tcatgatgtt gcccttgtgg acaagcgtca cggagtcctt cttgtggtcg
     8341 atcgcgtagc ggatggcctt gcgcaccagg cgcttggtgc cgaaggccga caccggcttg
     8401 atgccgagac cggcgccctc gaagaactcg gcgcccattt cctcacgcag gaacttggcg
     8461 accttctcgt tctcgggcgt gccggccttg tactcgatgc ccgagtacac gtcctcggtg
     8521 ttctcgcgga agatgacgat gtccacgagt tccgggtgca ccatcggcga gggcacgccg
     8581 gcgtagtagc gcaccgggcg cacgcaggca tacaggtcga gttcctggcg cagcgcgacg
     8641 ttcagcgagc ggaagccgcc accgaccggc gtggtcagcg gacccttgat cgagacgatc
     8701 aggtccttca gggcttccag ggtttcgtcc gggaagtagt tgccgtcgta gatctcggca
     8761 gccttctcgc ccatgaacag ctcgcaccag tgaatcttgc gcttgccgtc gtaggcctgc
     8821 tccaccgccg catcccacac gcgcagacag gcgcgggtga tgtcggggcc gatgccgtcg
     8881 ccctcgacgt agccgacgac cggctggtcc ggaacgctga gcttgccgtc gacgacggtg
     8941 atcttctcgc cggtggcggg catcttgatg tgctggtaag ccatgagacc tccttcctcg
     9001 tccgctcgct gcggcggaca gacttgttca cgcggcgcgt caggctggaa agacctgatg
     9061 acttgtcgcg acccccgatg ttcggggccg gaaacgttgc ggcgggatgg tccgtacagc
     9121 aaacgcgcag gtcgcaccgg caggccggag ctgccggacg ggtctgtcgc ctgcggagga
     9181 aggctcgcag ccggaaacgg ccgcgggagc cacgaatacg gtacgccgaa acgctgccaa
     9241 ccccttgccc ggccttgtcg atctgtgtcg atctcgttat ggatgccggg tgctccagcc
     9301 gcgcgcaaaa ctacacgcaa acgtgcgggg tggcaatcgc gccgccggcc gcctccggcg
     9361 gcgggccggg gccgaatggc gatttcgggg cgtgcagcag ggattgccgg aggcaggggc
     9421 ttttccggca ggtgaaaccg gcgggcgcgg cgcgtgcggg gcgcggattt cttgttgcgg
     9481 gcgccggcag gcggctacca tcggtgctcc ggtcggccag gaacggcgga ccgacccgcc
     9541 tgagagcgac cgcaagatga cgatgcgcac ctcgatcccc ggaatcgtcg ccggactggc
     9601 cctcaccggg atggccgccg cggccgtcgc ggccgacggc gcgtggatcc cgcgcggcaa
     9661 gccgatgcgg cagtttccgt cctgctacca gatgtgggtc tgcgtcgcga aggacaagcc
     9721 cgagggaccg ttcagcggca tgccgcgcgg cacctggggc gcctgcgaca ccagttatac
     9781 cgccggcgcg gccggcagcc aggccgaaag ctgcgagaaa tgcctcgccg gcccgcccga
     9841 ggacgcctgc aagccctgac gccggcccgg atcgcccacc ggctgcggct gcggcggcgt
     9901 tctcagccga ggaagcccgt acatgacgct gaccgaactg cgctacatcg tcgcggtggc
     9961 acgcgaacgc cacttcggcc gtgccgccga ggcctgcttc gtcagccagc cgacgctgtc
    10021 ggtggcggtg cgcaagctcg aggacgaact cggcgtcgtg ctgttcgagc gcggccccgg
    10081 cgaggtcacc gtcacgccgg tcggccggcg cgtggtcggg caggcgcagc gcgtgctgga
    10141 ggaagccgac gcgatccggg cgctggcgct gtcgggcaag gacgagctcg ccggcgtgct
    10201 gcgcctcggc gcgatctaca cgatcggccc ctacctgctg ccgcaactga tcccgcgcct
    10261 gaagcgcgtc gcgccgcaca tgccgctgca gatcgaggaa aactacacgg ccgtgctcgg
    10321 cgagcgcctg aagcagggca agctcgacgt gctgatcctg tcgctgccct tcgaggagag
    10381 cggcgtgctg accgaggcgc tgtacgagga gcccttcgtc gtcgtgatgc cggccggcca
    10441 cgcgctggag cacgagcgcg cgatcgacgt gcacgcgctc gcgcaggagg atctgctgct
    10501 gctcggcccc ggacactgct tccgcgacca ggtgctgcag gtctgccccg agtgcaaccg
    10561 cgccgcggtg gcctcggacg ccgacaacat gcagcgcacg ctcgaaggca gctcgctcga
    10621 aaccatccgg ctgatggtcg ccaccggcat gggcgtgacc gtgctgccgc agacttcgat
    10681 gaccgcctac gccagcgcgc ccgaactgct gagcgtgcgg ccgttcacgg aaccggcgcc
    10741 gaagcgcgtc gtcgcgctgg cctggcgcaa gagcttcccg cggccggcgg cgatccgcgt
    10801 gctggcggcg gccgtgcgcg cctgtccgct cggcgagtcc atcgaggtgc tggagggcgc
    10861 gacggcctga atccggcccg tcgccgcccc gtcccggccg ctggccggcg cgcaggcttt
    10921 tcagccgggg tcgcgatcga gcgcctgcaa gccttcgatc agcttcggca ccagcgctgc
    10981 ttcggccgca tcgatgccgg ccagcgcatg gcggatcagc gcccggccgc cggggctgcc
    11041 cggttcggcg tcgagttcgg cggccatgcg cccgaggttg ccgacgatca gccgcgtgat
    11101 gcgctggttc tcgccgaaca cgccggcggt ctggatcgcg gtggcaacca tttccgtggc
    11161 cgcctcgaag cggtcttttt cggtcatgcc cgtctccgtc gccggcgcct gccggcgacc
    11221 tgcggccgcg gccatcaggc gcgcgtgccg gcgtcgtagt tcttcacgcg ctgcccggcg
    11281 gcgacgccga ggatcttcga cagccacggc ggcggcgact tgatcatcgc ctgctggaac
    11341 tgcgcgatga cctcggcggc ctcgccgcct tcgggacgct tgatcggata gatgtcggcg
    11401 cggatcacct tcggcgccgc cgggccgccg atcgacacca cgtacagcac gtggcagtcg
    11461 ctgatcagcg aggtgcggaa ggcattcttg tcctcggcga agtcggcttc gagcgcgtcg
    11521 cggatgccga ccagccggct ctcggtcggc gagacctgat agacgaggaa gcgcaggcag
    11581 gagccgaagt ggccgttcag cgtcgtgcca ctgtccgagg cgagcgcgat gcgcaccgag
    11641 ccgggcatgt cgccctcggc gtaggactcg accggcggca ggtcggcggt gccgtcgtcg
    11701 tcgccccaga ggatgcgcac ggccatcttc atcgcgtcga ggccgatcag gatgtcctcg
    11761 ttgtcctcct cgccgtcgac gctgccgagg ccggtcttga ggtcggtgac ggtgacggtg
    11821 cgcagcttgt cggtgtcgat gtgatcgccg aggcggttgt gcagcacctg caccagctgc
    11881 gccagcgcga tgttcggcat ggcgcgcgca gcgagggcga cgcgcagcgc ggcttcctgc
    11941 gtgatgggct cgacttcgga ctcggacatc gcggcatctc cgggggcagt gagggggcgg
    12001 tcgtaccgga aatgctgcac tgcacaggcg atgccagtcc ggcacgattg cgtaacaacc
    12061 tgatcctcat caggaaacca cgccccgggc tcgcgccccc gcattgtcgg cccctgtcgg
    12121 gtttgcgaca ggtcgcgcgc gcgccggtgc cgggcgctat acgtcagcga cacgagcact
    12181 ccc
//
LOCUS       DAOQIE010000085        12019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2604_length_12019_cov_7.902708, whole genome shotgun sequence.
ACCESSION   DAOQIE010000085 DAOQIE010000000
VERSION     DAOQIE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_12019_cov_7.902708"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PK144_07105"
     CDS             complement(<1..1822)
                     /locus_tag="PK144_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65532.1"
                     /translation="MTLQSDNANALNVTGNFAIGKNGNGIFQQSAGAVSAAQVLLGGE
                     VFGTDTSNGNGQYTMSGGSLTTSTLTVGNLGSGSFTQSGGDVSTSQLTLGSAGGTPAN
                     SFGQYFLSGSGTLTTTQSTIGDIGRGEFTQSGGTHTVASDLVLGNAPALTGGTPARGG
                     FYTLGGGQLIVAGSTIIGNGNNGFAGEPGGSGSFTQSGGSQFVGGELRVGNTGSDAGG
                     TGSYVLSGTGSLTVAGTLRLGAAGSGFGGTGSFTQTGGSVSASNLSIGESDTTSGGTS
                     SYTLSGGTLAITSNVLVGAGADGTFTQSGGVFTVGNDLSINTGSYALSGGSVGIGTSL
                     HLGSDHAATLLQSGGSLDVGDQAFIGAGAGGNGSYTLSAGTLSADYLDVAIVAGSTGT
                     LTIRGGSATARVNLVIGSDATTTASLVQTGGSVSVQSASFGLYVNGSYRLNDTDGPAS
                     LDVTHDAVIGDTLSGSFTQVQGAVTVGHDLILGSGTSAGANGSYAISGGSLDVDGRVI
                     IGQTTGVSGQYTQSGGTVTVGGDFVVANLSGSHGTATLSAGSLSVHGTATDSRIVVGG
                     LGSGSFTLSGGSVTADGLSVGNNAGGSGTFTQTGGSVFVQS"
     gene            complement(2299..2874)
                     /locus_tag="PK144_07110"
     CDS             complement(2299..2874)
                     /locus_tag="PK144_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65533.1"
                     /translation="MNTAARLAERLLPLLAATRQPRAANSETPALPADEAFLAVRGPE
                     RWLALNGASRAAAQSLGEALAGDDAAALEAALDALVASTGDWLALHAELMQSEAADAQ
                     LAEGRRLALAVVARALSDLALWLARVLAGLERQPPAPLPPLRLHAQAEVLRWAAWNGR
                     RTLTQPLPAPGDGSAGSGWIHQLRRWLSSHF"
     gene            complement(2931..3788)
                     /locus_tag="PK144_07115"
     CDS             complement(2931..3788)
                     /locus_tag="PK144_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF65534.1"
                     /translation="MKRILIAHRTEYRYPEPVRLGPHRLLLRPREGHEVRVERSRLDI
                     TPAAQLRWCRDADDNSVAIAHFPQPAGRLCIDSEVVIEHHDDAPLDFLVEDYALHYPF
                     EYAAAERTDLQLASTPVYPPDRAVVAAWVTGLGIGAGTLETYVLLDRLNRAICGQLEY
                     GMREEPGVQSPAETLRLGTGSCRDYATLFIEACRGLGLASRFVSGYLHTSETAAGHAS
                     THAWAEVYLPGPGWKGFDPTGGVLTGSDHIPVAVARHPEAIPPVSGSFYGPPDLQADM
                     QVDVRVAAL"
     gene            complement(3826..5541)
                     /locus_tag="PK144_07120"
     CDS             complement(3826..5541)
                     /locus_tag="PK144_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNF65535.1"
                     /translation="MSPALRRWLPFLAWPRPTRATLAADLRAGLAVGLVLIPQALAYA
                     TLAGMPAQTGLYAALLPAVVGCLWGSSPLLAAGPVALTSLLSFGALQPFAAPGSSGWV
                     AAAVWLALYAGLMQCALGALRLGVIANLVSGPTLAGFIHAAALIIIATQLPALLGLPP
                     WGASLSGWPAQVAARLQQAPGAVAVSAAFGLGALLLLLWLRRHRPHWPGVLLVCGLGI
                     VLSHFSAYAGHGGAVIGSVPEALPQLSLPPALDFGTHRALLAPALVVALVSFTEAMSS
                     ARTLARRRGERWNENQELIGQGLAKIASAASGAFPVSGSFSRSALNEYAGARTAWSTL
                     FAAACVFAFLLAGTAVLAPLPQAVLAAVVIVPVTGLINVAGLRRIGRLARDDGLVALI
                     TFAATLFAVPQLHWGVLAGLIAGLAASMLRRSQPRIVELGVLPDGRLRARERYGLPRP
                     APDLIVLRPDTALVYASAAAVERAVLDHVDAGVQRVLLVGTAINDLDVTGLDTLRELR
                     RTLAARGTTLYLCRMKLPVWRLLERAGVPAEFGENRCFRTERQAIAALTGENPAPAAA
                     DRPHP"
     gene            5760..6869
                     /locus_tag="PK144_07125"
     CDS             5760..6869
                     /locus_tag="PK144_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNF65536.1"
                     /translation="MKARAAVAWEAQKPLSVTEIEVEGPRAGEVLLRTVATGVCHTDA
                     FTLSGDDPEGVFPCVLGHEGGCIVEECGPDVKGLKPGDHVIPLYIPECGHCEYCASTK
                     TNLCQSIAATVWTGYMPDGTRRFAKDGKPIFHYMGCSTFSEYTVVPEIALAKINPAAP
                     LDKVCLLGCGITTGIGAVLNTAKVEPGSTVAVFGLGGIGLSVIQGAVMAKAGRIIAID
                     TNPAKFDMARALGATDCVNPEALTVSTREAIVEMTHGGVDYSFECIGNVEVMREALEC
                     THMGWGVSTIIGVAGAGREIRTRPFQLVTGRTWKGTAFGGVKGRSQLPGFVDQYLNGT
                     IRIDPMVTHTMGLEDINHAFELMHEGKSIRSVIVF"
     gene            6932..7774
                     /gene="fghA"
                     /locus_tag="PK144_07130"
     CDS             6932..7774
                     /gene="fghA"
                     /locus_tag="PK144_07130"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNF65537.1"
                     /translation="MSTPERLERIREHEGWLERWRHDSQSCACPMTFSIYLPPQAEAG
                     PVPAVYWLSGLTCSDDNFRVKAGAQRYAAELGLALLIPDTSPRGDEVPDDPGYDLGQA
                     AGFYVEAIRAPWATHYHMHDYVAFELPLLAETLFPLQPGRRAICGHSMGGHGALVLAL
                     RHPGRYSSVSAFAPICHPSASPWGEKAFTAYLGADRRMWRPWDATSLIEDGATPLPMR
                     IEQGTADEFLGEQLHPDDLAAACAARGIPLDLRLRPGYDHSYHFIASFIGEHLAWHAG
                     HLQG"
     gene            7904..8935
                     /locus_tag="PK144_07135"
     CDS             7904..8935
                     /locus_tag="PK144_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNF65538.1"
                     /translation="MQARLRASGPSLIAVAGAVLGLFVYERLPALAAALAAWLPFAPD
                     SRAGAAIGFFLYEVPKVLLLLCAVVFVMGIVRSYCSPERTRALLAGRRGGLGNALAAA
                     LGVVTPFCSCSAVPLFIGFLGAGVPLGVTLSFLIAAPMVNEIALGLLFALFGWRIAAL
                     YLALGLTVALGAGWIIGRIGMEGSVEDWVRTCPATTAAAGLSGPVGWNAHLGAGSAAV
                     RDIVGRVWPYLLAGIAVGAGIHGYVPTDFMAALMGREAWWSVPLAVALGVPMYSNAAG
                     IVPVVEALLAKGAALGTALAFMMSVVALSLPELVILRKVLKPRLIAVFTGVVATGILL
                     VGYVFNLVL"
     gene            8971..9210
                     /locus_tag="PK144_07140"
     CDS             8971..9210
                     /locus_tag="PK144_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF65539.1"
                     /translation="MNRRKIQILGSGCQKCRKLAEYATQAADELGLDWELEKITDPAR
                     IAGFGVMQTPALAVDGVVKLAGHLAGVAELKRLLG"
     gene            complement(9240..10562)
                     /locus_tag="PK144_07145"
     CDS             complement(9240..10562)
                     /locus_tag="PK144_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNF65540.1"
                     /translation="MSSVPGAAPPPAFLFGGLREHATRRVLDHEIHARPSEELRAPAR
                     ASHLAVLSGPDGAAADRAHVAELCWRYGVEPPASNEVFHAIDLGPLRLRWERHTEFSS
                     YTFLRAEPDQDDPFAAPPIASVPSDWLAGLPGEVLVATHVILESGGSAGRDLANMQAV
                     FGTTNIAGSRVLGGAAVAWSDFRIHPDGFDRLLVADHGLSPRQAGRLVQRLLEIETYR
                     MLAMLGFPNARRSSQELNGVERRLAEITGRMTGNEAPDAEAKLLTELSDLAAEVERGA
                     ATSTYRFAASRAYRTLVERRIEELREERIEGLQTLEEFMERRLAPAMRTVEAVRERQS
                     QLSERVARATGLLRTRVDVTLQQQNQALLASMNRRADLQLRLQETVEGLSVVVLSYYL
                     VSLLGYLVKALSKAGLPLNPEIVSGAAVPVALAALTWKIGRMRKALAH"
     gene            10757..11446
                     /gene="phoU"
                     /locus_tag="PK144_07150"
     CDS             10757..11446
                     /gene="phoU"
                     /locus_tag="PK144_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNF65541.1"
                     /translation="MSFTTEPHLVRSYDERLASLRDLLTRMGSLAATQFEAAAAALLA
                     LDAGIAARWPHGERCIDELDAEIERALLLLLALRAPVASDLRLVLALADAARHLERIG
                     DETGKLAAATVALAGTPCPRSLLDAAVRPLLAQVGDMLAQARSALDAVDATAAARLVE
                     DDRRLDAEYHARLATLVAWMAEHPRGIGAGMQLASALKAMERIGDHARDIGLRIAACV
                     RGTAGEAAATG"
     gene            complement(11488..11763)
                     /locus_tag="PK144_07155"
     CDS             complement(11488..11763)
                     /locus_tag="PK144_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65542.1"
                     /translation="MSLRPRASLLPRLAVPLFAGLLSVSFVASAAPPPPAEPAKLHAA
                     MEALKLAQRDLEAAPSDHGGHRNKALQLIERALEQCERSAESLRQAH"
BASE COUNT         1729 a         4311 c         4279 g         1700 t
ORIGIN      
        1 ccgactgcac gaacacgctg ccgccggtct gcgtgaacgt gccgctgccg ccggcgttgt
       61 tgccgaccga caggccgtcg gcagtcacgc tgccgccgga gagcgtgaac gaaccgctgc
      121 cgaggccgcc gacgacgatg cggctgtcgg tggccgtgcc atgcacgctc aggctgccgg
      181 cactgagggt ggctgtcccg tggctgccgc tcaggttggc gacgacgaaa tccccgccga
      241 ccgtgaccgt gccgcccgac tgcgtgtact gaccgctgac gccggtcgtc tggccgatga
      301 tgacccgccc gtcaacatcg aggctgccgc ccgagatcgc gtagctgccg ttggccccgg
      361 cgctcgtgcc gctgccgagg atcaggtcat ggccgaccgt cacggcaccc tgcacctgcg
      421 tgaagctgcc gctgagggtg tcgccgatca cggcatcgtg cgtgacatcg agcgacgccg
      481 gaccgtcggt gtcgttcagg cggtagctgc cgttgacgta caggccgaag ctcgccgact
      541 gcacggacac gctgccgccg gtctgcacga ggctcgccgt cgtcgtcgcg tcgctgccga
      601 tgacgaggtt cacgcgtgcg gtcgcgctgc cgccgcggat ggtcagggtg ccggtactgc
      661 ccgccacgat cgccacatca aggtaatcgg cgctcagcgt gccggcactg agcgtatagc
      721 tgccgttgcc gccggcaccg gcgccgatga aggcctggtc accgacatcg agcgaaccgc
      781 cgctctgcag cagcgtggcc gcgtgatccg agcccagatg cagcgaggtg ccgataccca
      841 cgctgccgcc cgacagtgcg tagctgccgg tattgatgct caggtcattg ccgaccgtga
      901 acaccccgcc ggactgcgtg aacgtgccat cggcgccggc accgaccagc acgttgctgg
      961 tgattgccag cgtgccgccg gacagcgtgt agctgctcgt gccgccgctg gtcgtatcgg
     1021 attcaccgat actcaggttc gaggcgctca cgctgccgcc cgtctgcgtg aagctgccgg
     1081 taccgccgaa gccgctgcca gccgcgccga ggcgcagcgt gccggcaacc gtcaggctgc
     1141 cggtgccgga gagcacataa ctgccggtgc cgccagcatc gctgccggta ttaccgaccc
     1201 gcagttcgcc gccgacgaac tgcgagccgc cgctctgcgt gaagctgccg ctgccgccgg
     1261 gttcgccggc aaagccgttg ttgccgttgc cgatgatggt gctgccggcg acgatcagct
     1321 ggccgccgcc gagcgtgtag aagcccccgc gggccggcgt gccgccggtc agcgccggcg
     1381 cgttgccgag caccagatcg ctggcgaccg tatgcgtgcc gccgctttgc gtgaactcgc
     1441 cacggccgat gtccccgatc gtcgactgcg tggtggtgag cgtgccgctg ccggacaaaa
     1501 aatactggcc gaagctgttg gcgggcgttc caccggcgct gcccagcgtc agctgcgagg
     1561 tcgaaacgtc gccgccgctc tgcgtgaagc tgccgctgcc cagattgccg accgtcagcg
     1621 tgctcgtcgt caggctgccg ccgctcatcg tgtactggcc gttgccgttg ctggtatcgg
     1681 taccgaacac ctcgccgcca agcaagacct gcgcggcgct cacggcgccg gcggactgct
     1741 ggaagatacc gttgccgttc ttgccgatgg cgaagttgcc agtaacattc aacgcattgg
     1801 cattgtcact ttgcagcgtc atcgagtccg gtgcagtggc atcgatcgtc agcgaggcat
     1861 aagccggagc gccagtctca tcgtaggtga cggtctgcga tcccagattg ctgccggccg
     1921 gggccagcag ctgcacgatg tccccggctt gcggactgcc ggaaatgtca tcccagaaac
     1981 cggtagtcca agtttcgcca tcattacctc cccaagtgac ggttgagggt gccgtctgcg
     2041 caggtgcctg ctgcatggcc agcccgagcg ccagcgacag caacgtgcgg ccggtgggga
     2101 agcgcttgca cgctgacgac agcccgggta gcggattgac aatgttcatc gctggggact
     2161 ccgtctctgc gggcggtcat gccggtcacc accgatgcag cggcgcggac gtggggtatg
     2221 ccacgatggt aacccgatgc ccgccacagt cggcatagcc ggatgcggct gtaacggccg
     2281 gtcaggcgga aacgccggtt agaagtgcga cgacagccag cgtcgcagtt gatggatcca
     2341 gcccgatccg gctgatccat cgccgggcgc cggcagcggt tgcgtcagcg tccgccgtcc
     2401 gttccacgcc gcccagcgca gcacctcggc ctgcgcgtgc aggcgcagcg gcggcagtgg
     2461 tgccggcggc tggcgttcga ggccggcgag cacgcgggcg agccagagcg ccagatccga
     2521 cagcgcccgc gccacgacgg cgagcgccag gcgccggcct tcggcgagct gcgcatcggc
     2581 ggcttcggac tgcatcagct cggcgtgcag cgccagccag tcgccggtgc tggcgaccag
     2641 cgcatcgagt gcggcttcga gcgccgcggc atcgtcgccg gcgagcgcct cgccgaggga
     2701 ttgcgccgcg gcgcggctgg cgccgttcag cgccagccag cgctccggcc cgcgcacggc
     2761 gaggaaggct tcgtcggccg gcagcgcggg ggtctcgctg ttcgcggccc gcggctggcg
     2821 ggtggcggcg agcagcggca gcaggcgttc ggcgaggcgg gcggcggtgt tcatcggcgg
     2881 ttccctcgcg gtggctgcgg acaggatgcg gtggggctgc cggcacagcc tcagagcgcc
     2941 gccacgcgca cgtcgacctg catgtccgcc tgcagatccg gtggtccgta gaaactgcct
     3001 gacaccggcg gaatggcttc cgggtggcgg gcgaccgcga ccgggatgtg gtcgctgccg
     3061 gtcagcacgc cgccggtcgg gtcgaagccc ttccagcccg gccccggcag gtagacctcg
     3121 gcccaggcgt gggtcgaggc atggccggcg gcggtctcgg aggtgtgcag gtaaccgctg
     3181 acgaagcggc tcgccagccc gaggccgcgg caggcctcga tgaacagcgt cgcgtagtcg
     3241 cggcaggagc cggtgccgag gcgcagcgtt tcggccggcg actgcacgcc gggctcctcg
     3301 cgcatgccgt attccagctg cccgcagatg gcgcggttca gccggtcgag cagcacgtag
     3361 gtttccagcg tccccgcccc gatgccgagt ccggtgaccc aggccgccac cacggcgcga
     3421 tcgggcggat agaccggcgt gctcgccagc tgcaggtcgg tgcgttcggc cgctgcgtac
     3481 tcgaacgggt agtgcagcgc gtagtcctcg accagaaagt cgagcggcgc atcgtcgtgg
     3541 tgttcgatga ccacttcgct gtcgatgcag agccgtccgg ccggctgcgg gaagtgcgcg
     3601 atcgcgaccg agttgtcatc ggcatcgcgg caccagcgca gctgcgcggc cggggtgatg
     3661 tcgaggcgcg agcgttcgac gcgcacctcg tggccctcgc gcgggcgcag cagcagccgg
     3721 tgcgggccga gccggaccgg ctcgggatag cggtattcgg tgcggtgcgc gatcaggatg
     3781 cgcttcatcg gcggctctcc ggcggcgatg cggacctgca ccgtactacg gatgcgggcg
     3841 atcggccgca gccggcgcag ggttttcgcc ggtcagcgcc gcgatcgcct gacgctcggt
     3901 gcggaaacag cggttttcgc cgaactccgc cggcacgccg gcgcgctcca gcagccgcca
     3961 gaccggcagc ttcatgcggc agagatagag cgtcgtgccg cgtgccgcca gcgtgcggcg
     4021 cagctcgcgc aaggtatcga ggccggtgac atcgagatca ttgatcgccg tgccgaccag
     4081 cagcacgcgc tgcacgccgg catcgacgtg atcgagcacc gcgcgctcga ccgccgccgc
     4141 actggcgtag accagcgccg tgtccgggcg cagcacgatc aggtccggcg ccggccgcgg
     4201 caggccgtag cgctcgcgcg cgcgcagccg gccatcgggc agcacgccga gttcgacgat
     4261 gcgcggctgg ctgcggcgca gcatcgaggc cgcgaggccg gcgatcaggc cggcgagcac
     4321 gccccagtgc agctgcggca ccgcgaacag cgtcgccgcg aaggtgatca gcgccaccag
     4381 gccgtcgtcg cgcgccagcc gcccgatgcg ccgcaggccg gccacgttga tcagcccggt
     4441 caccggcacg atgacgacgg ccgccagcac cgcctgcggc agcggcgcga gcacggcggt
     4501 gccggcgagc aggaacgcga acacgcaggc agcggcgaac agcgtcgacc aggccgtgcg
     4561 ggcgccggcg tattcattga gggccgagcg cgagaacgag ccgctgaccg gaaacgcccc
     4621 gctggcggcg ctcgcgatct tggcgagccc ctgaccgatc agctcctgat tctcgttcca
     4681 gcgctcgccg cgccggcgcg ccagcgtgcg cgcactcgac atcgcctcgg tgaagctgac
     4741 cagcgccacg accagcgccg gcgcgagcag cgcccggtgc gtcccgaaat cgagcgccgg
     4801 tggcagcgac agctgcggca gcgcctcggg cacgctgccg atcacggccc cgccatggcc
     4861 tgcgtaagcg ctgaagtggc tgagcacgat gccgagcccg cagacgagca gtacgcccgg
     4921 ccagtgcggc cggtgccggc gcagccacag cagcagcagc agggccccga gcccgaaggc
     4981 cgcggacacg gccaccgcgc cgggcgcctg ctgcaggcgc gccgccacct gcgccggcca
     5041 gccggacagc gacgcccccc agggcggcag gccgagcagc gccggcagct gcgtggcgat
     5101 gatgatcagc gccgccgcat ggatgaagcc ggcgagcgtc ggccccgaga ccaggttcgc
     5161 gatcaccccg aggcgcagcg ccccgagtgc gcactgcatc aggcccgcgt acagcgcgag
     5221 ccacaccgcg gccgccaccc agccgctgct gcccggcgcc gcgaacggct gcagcgcgcc
     5281 gaagctcagc aggctggtca gcgccaccgg cccggcggcg agcagcggcg acgagcccca
     5341 caggcagccg accaccgccg gcagcagcgc cgcgtacagc ccggtctgcg ccggcatgcc
     5401 ggcgagcgtc gcgtaggcga gcgcctgcgg gatcagcacc agcccgaccg cgagcccggc
     5461 gcgcaggtcg gcagcgagtg tcgcgcgcgt cggacgcggc caggcgagga agggcagcca
     5521 gcggcgcagg gcaggggaca tgatcggcga aggcctccgg cggcggtggc ggacgggtat
     5581 ccagcctgac aggaaccggc gcccgccgcc cgcagtcctg ctgagaccag ccattccccg
     5641 gcgtgccggg gccggcggtc gcctgcgcat ccccgatgct gccgtctagc ctgtagcggt
     5701 tcgagccgtc cgcggccgcc cgatgcctgc gggcatcgcc ccacacgagg agcctgccga
     5761 tgaaagcccg tgccgccgtt gcctgggaag cgcagaagcc gctctcggtc accgagatcg
     5821 aagtcgaggg gccgcgtgcc ggcgaagtgc tgctgcgcac ggtcgccacc ggggtctgcc
     5881 acaccgatgc cttcacgctg tccggcgacg accccgaggg cgtcttcccg tgcgtgctcg
     5941 gccacgaggg cggctgcatc gtcgaggagt gcgggccgga cgtgaagggc ctgaagcccg
     6001 gcgatcacgt gatcccgctg tacatccccg aatgcggtca ctgcgagtac tgcgcctcga
     6061 cgaagaccaa cctctgccag tcgatcgccg cgacggtctg gaccggctac atgcccgacg
     6121 gcacgcgccg cttcgcgaag gacggcaagc cgatcttcca ttacatgggc tgctcgacct
     6181 tcagcgaata cacggtcgtg cccgagatcg cgctcgcgaa gatcaacccg gccgccccgc
     6241 tcgacaaggt ctgcctgctc ggctgcggca tcaccaccgg catcggcgcg gtgctgaaca
     6301 ctgcgaaggt cgagcccggc agcacggtcg cggtgttcgg gctcggcggc atcggcctga
     6361 gcgtgatcca gggcgcggtg atggcgaagg ccggccgcat catcgccatc gacaccaacc
     6421 cggccaagtt cgacatggcg cgcgcgctcg gcgccaccga ctgcgtgaac ccggaggcgc
     6481 tcacggtcag cacgcgcgag gccatcgtcg agatgacgca cggcggcgtc gactacagct
     6541 tcgaatgcat cggcaacgtc gaggtcatgc gcgaggccct ggaatgcacg cacatgggct
     6601 ggggcgtgtc gacgatcatc ggcgtcgccg gtgccggccg cgagatccgc acccggccgt
     6661 tccagctcgt gaccggccgc acctggaagg gcacggcctt cggcggcgtc aagggtcgct
     6721 cgcagctgcc gggtttcgtc gatcagtacc tgaacggcac gatccgcatc gacccgatgg
     6781 tcacgcacac gatgggtctg gaagacatca accatgcctt cgagctgatg catgaaggca
     6841 agagcatccg ctcggtgatc gtgttctgaa tgaaccggcc gccgcttcgc ccggctttcc
     6901 ccgtccttac ctgccgcgag gccccgcccc gatgtcgacc cccgaacgcc tcgaacgcat
     6961 ccgcgagcat gaaggctggc tcgaacgctg gcggcatgac tcgcaaagct gcgcctgtcc
     7021 gatgacgttt tcgatctacc tgccgccgca ggccgaggcc ggcccggtgc cggccgtgta
     7081 ctggctgtcg ggcctgacct gctccgacga caacttccgc gtcaaggccg gcgcacagcg
     7141 ctacgccgcc gaactcggcc tggcgctgct gattccggat accagcccgc gcggcgacga
     7201 ggtgccggat gatcccggct acgacctcgg ccaggccgcc ggcttctacg tcgaggcgat
     7261 ccgcgcaccg tgggccacgc actaccacat gcacgactac gtcgccttcg aactgccgct
     7321 gctcgccgag accttgttcc cgctgcagcc gggccggcgg gcgatctgcg gccactcgat
     7381 gggcgggcac ggcgcgctgg tgctggccct gcgccatccg gggcgctaca gcagcgtctc
     7441 ggccttcgca ccgatctgcc atccgtcggc gagcccctgg ggcgagaagg cgttcacggc
     7501 ctatctcggt gccgaccggc gcatgtggcg gccctgggat gcaaccagcc tgatcgagga
     7561 cggcgccacg ccgctgccga tgcgcatcga gcagggcacg gccgatgagt tcctcggcga
     7621 gcagctgcac ccggacgacc tcgccgccgc ctgcgcggcg cgcggcatcc cgctcgatct
     7681 gcgcctgcgt cccggctacg accacagcta ccacttcatc gccagcttca tcggcgagca
     7741 cctcgcctgg cacgccgggc atctgcaggg ctgaagccgg gctgcacgac aacctccgga
     7801 gcgattgaca ccggtcatcg cgcgcttgcc tgcattgttc tatggatatc gaaacaaatg
     7861 caggagcgcc gcgatgcaga ccgttccgtc cgttccgtcg tcgatgcagg cgcgactgcg
     7921 cgccagcggc ccgagcctga tcgccgtggc cggcgccgtg ctggggctgt tcgtgtacga
     7981 gcggctgccg gcactcgccg cggccctggc tgcctggctg ccgtttgcgc ccgacagccg
     8041 ggccggggcg gccatcggct tcttcctgta cgaagtgccg aaggtcctgc tgctgctgtg
     8101 cgccgtcgtg ttcgtgatgg gcatcgtgcg ctcgtactgc tcgcccgagc gcacgcgggc
     8161 gctgctcgcc gggcgacgcg gcggcctcgg caatgccctc gccgccgcac tcggcgtggt
     8221 cacgccgttc tgctcgtgct cggccgtgcc gctgttcatc ggcttcctcg gggccggcgt
     8281 gccgctcggc gtgaccctgt cgttcctgat cgccgcaccg atggtcaacg agatcgcgct
     8341 cggcctgctg ttcgcgctgt tcggctggcg gatcgcggcg ctgtatctgg cgctcggcct
     8401 gacggtcgcg ctcggggcgg gctggatcat cggccgcatc ggcatggagg gcagcgtcga
     8461 ggactgggtg cgcacttgcc cggccacgac cgccgcggcc ggtctttccg gcccggtcgg
     8521 ctggaatgca catctcggcg ccggcagcgc cgccgtgcgc gacatcgtcg ggcgggtctg
     8581 gccttacctc ctcgccggca tcgcggtcgg cgccggcatc cacggctacg tgccgaccga
     8641 cttcatggcg gcgctgatgg gccgcgaggc gtggtggagc gtgccgctcg ccgtcgcgct
     8701 cggcgtgccg atgtactcga atgccgccgg catcgtgccg gtggtcgagg cgctgctcgc
     8761 caagggcgcg gccctcggca cggcactggc gttcatgatg tcggtcgtcg cgctgtcgct
     8821 gcccgagctg gtgatcctgc gcaaggtgct gaaaccgcga ctgatcgcgg tgttcaccgg
     8881 tgtggtcgca accggcatcc tgctggtcgg ctacgtgttc aatctcgtgc tgtgacgagg
     8941 ccggtcaccg accggctgcg gaggcctgcc atgaaccgac gcaagatcca gatcctcggc
     9001 agcggctgcc agaaatgccg caagctcgcc gaatacgcga cgcaggccgc cgacgagctg
     9061 ggcctcgact gggagctgga gaagatcacc gatccggcgc gcatcgccgg tttcggcgtg
     9121 atgcagaccc cggccctcgc cgtcgacggc gtcgtgaagc tcgccggcca cctcgccggt
     9181 gtcgccgagc tgaagcgcct gctcggctga ggatccgcga cgaagggccg gagggggcat
     9241 cagtgcgcga gcgccttgcg catgcggccg atcttccagg tcagcgccgc cagcgcgacc
     9301 ggcaccgcgg ccccggagac gatctccggg ttcagcggca gtccggcctt cgacagcgcc
     9361 ttgaccagat agccgagcag gctgaccagg tagtagctga gcacgaccac cgacaggccc
     9421 tcgaccgtct cctgcagccg cagctgcagg tcggcgcggc ggttcatcga cgcgagcagc
     9481 gcctggttct gctgctgcag cgtcacgtcg acgcgcgtgc gcagcagccc ggtcgcccgc
     9541 gcgacgcgct cggacagctg gctctggcgc tcgcgcacgg cctcgaccgt gcgcatcgcc
     9601 ggcgccagcc gccgctccat gaactcctcc agcgtctgca gaccctcgat gcgctcctcg
     9661 cgcagctcct cgatgcgccg ctcgaccagc gtgcggtagg cacggctggc cgcgaagcgg
     9721 taggtcgagg tcgccgcccc gcgctcgacc tcggcggcga ggtcggagag ctcggtcagc
     9781 agcttcgcct cggcgtccgg ggcctcgttg ccggtcatgc gtccggtgat ctcggccagc
     9841 cggcgttcga cgccgttgag ctcctgcgaa ctgcgccggg cattcgggaa gccgagcatc
     9901 gccagcatcc ggtaggtctc gatctccagc aggcgctgca ccagccgccc ggcctgacgc
     9961 ggcgacaggc cgtgatcggc gaccagcagc cggtcgaagc cgtccggatg gatgcggaag
    10021 tcgctccagg ccacggccgc gccgccgagc acgcggctgc cggcgatgtt ggtcgtgccg
    10081 aacaccgcct gcatgttggc gagatcgcgc ccggcgctgc cgccggattc gaggatcacg
    10141 tgcgtggcga ccagcacctc gccgggcagg ccggcgagcc agtccgaggg caccgaggcg
    10201 atcggcggcg ccgcgaacgg gtcgtcctga tccggctcgg cgcgcaggaa ggtgtaggac
    10261 gagaactcgg tgtgccgctc ccagcgcagg cgcagcggcc cgaggtcgat ggcgtggaac
    10321 acctcgttcg aggccggcgg ctcgacgccg tagcgccagc acagctcggc gacgtgggcg
    10381 cgatcggccg ccgcgccgtc cgggccggac agcacggcca gatgcgaggc gcgcgccggg
    10441 gcgcgcagtt cctcggacgg ccgggcatgg atttcgtggt cgagcacgcg gcgggtggcg
    10501 tgttcgcgca ggccgccgaa caggaacgcc ggcggtggtg ccgcgccggg gacgctggac
    10561 atggtgggac tccggaaggg tctggaagcg ggagcggcga gtctacgggg cacatgccat
    10621 cgtgaaaagc attgcgtcag atgaaaatac acctgccacc ccggcgtcct gcgctggtca
    10681 cgggcttgtc atgttgcagc gcaataatgc ggcggccgac ccggccgttc cagtccctgc
    10741 cttgcggatt gccaccatga gcttcacgac cgaaccccac ctcgtgcgca gttacgacga
    10801 gcgcctggcc agcctgcgcg acctgctgac gcgcatgggc agcctggccg cgacgcagtt
    10861 cgaggccgcc gccgccgcac tgctcgcgct cgatgccggc atcgccgccc gctggccgca
    10921 cggcgagcgc tgcatcgacg agctcgatgc cgagatcgaa cgcgcgctgc tgctgctgct
    10981 ggcgctgcgc gcgccggtgg cctcggacct gcgcctggtg ctggcgctcg ccgatgccgc
    11041 gcgccatctg gagcgcatcg gcgacgagac cggcaagctc gctgccgcca ccgtcgcgct
    11101 cgccggcacg ccctgcccgc gcagcctgct cgatgcggcg gtgcgcccgc tgctggcaca
    11161 ggtcggcgac atgctcgcgc aggcgcgttc ggccctcgat gcggtcgatg ccacggctgc
    11221 ggcgcgactg gtcgaggacg accggcggct ggatgccgaa tatcacgcgc ggctggcgac
    11281 gctggtcgcg tggatggccg agcatccgcg cggcatcggc gccggcatgc agctcgccag
    11341 cgcgctgaag gccatggagc gcatcggcga tcatgcccgc gacatcggcc tgcgcatcgc
    11401 cgcctgcgtg cgcggcacgg ccggcgaagc ggccgcgacc ggctgagcgc acgaacgccg
    11461 ccggccctca ggcccggaaa tcagcgctca gtgcgcctga cgcagcgatt cggcgctgcg
    11521 ctcgcactgt tcgagcgcgc gctcgatcag ctgcagcgcc ttgttgcgat gcccgccgtg
    11581 atcggacggc gccgcctcca gatcgcgctg cgcgagcttc agcgcctcca tcgccgcgtg
    11641 cagcttcgcc ggctcggccg gcggaggcgg cgcggcactc gcgacgaagg acacggacag
    11701 caggccggcg aacagcggca cggcgaggcg cggcagcagc gaggcacgcg ggcgcaatga
    11761 catcgggacg acctcggacg ggcgcaggga aaggatgcag ccatgctacg cccgccgcgt
    11821 ccccgacggc ttgaagccgg cttaagcccg gtcgcatcag gcggcgcgct gcagatcatc
    11881 ctgctgcgat cgcagcgcac gcccggccct gcgcgcgccg caggagcgtc aggccatgca
    11941 gccgctgccg gaaaccttcg ctccccgctg cggcaaggcc ggcctcagag cggcttgcgc
    12001 agcgtgaacg cgccgcgca
//
LOCUS       DAOQIE010000086        11921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2630_length_11921_cov_7.053430, whole genome shotgun sequence.
ACCESSION   DAOQIE010000086 DAOQIE010000000
VERSION     DAOQIE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11921
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2630_length_11921_cov_7.053430"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PK144_07160"
     CDS             <1..854
                     /locus_tag="PK144_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF65543.1"
                     /translation="FHGGSRRRSPRRALHGVAAWLATLGCALPLAIGFVLPAGRLLAL
                     AHADGDAQLGPRFLTLAGNSLAISALTAALATLLALALAYGARLARGPARRAAGLLNR
                     GIALGYAVPGSVIAVGILIPVTRLDHAIAALWQSLGQPHPGLLLTGGLAALVYGYLVR
                     FLSVAVQTVDTGLSRITVSMDEAARSLGRGRGALLREVHLPLLRGSLASAALLVFVDT
                     MKELPATLVMRPFNFDTLATQVYTLAADERLAEASTAALAIVAVGVLPLILLSRHIAQ
                     GTQPQDS"
     gene            945..1535
                     /locus_tag="PK144_07165"
     CDS             945..1535
                     /locus_tag="PK144_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNF65544.1"
                     /translation="MARRILILDGHPDRDPAPHFLPALLDAYADGARAAGHEVERLRV
                     ATLDFPWLRSGGDWEPANTPADVTRLQAALTAADHWLLAYPLWFGNPPALLLGLLEHA
                     LRPGFAFTPPGPDAPPKPLLGGRSAHVLVTMNMPAFAYRWYFGAHGVKTLRRHLLGTA
                     GFRPVRTTLVGSVLTIGTGGRERWLQRLRAMGTEGR"
     gene            1584..2042
                     /locus_tag="PK144_07170"
     CDS             1584..2042
                     /locus_tag="PK144_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65545.1"
                     /translation="MLSAPTAEATTAAAMRPASTALLLLAALAALPGCSTARLPPPED
                     LAGVPGYYASGRRGAVYYDVAIYLDADNRGAVVMDGGCEGPPRILPQTVVRSEGYVSF
                     QAMGCRDPRYAGVEVTQAELADGKVLGAELYLLRRRDSVLERGPSMTLSP"
     gene            complement(2115..2645)
                     /locus_tag="PK144_07175"
     CDS             complement(2115..2645)
                     /locus_tag="PK144_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNF65546.1"
                     /translation="MPSRRHVLLGLGAFTFTSAFSSLLAGCGEDRPPAVALRPVPIAA
                     ADECAVCGMQIRGFPGPKGEAHVSGREAALKFCSTRDFFAWVLQPEVGASVQALWVHD
                     LGATGWEQPDDAAFTDARSAFYVIGHRRLGAMGPTLASFRERPAAEAFAAREGGRVLA
                     FLDVTVALVAGGLEPG"
     assembly_gap    2662..2761
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2762..3595)
                     /locus_tag="PK144_07180"
     CDS             complement(2762..3595)
                     /locus_tag="PK144_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF65547.1"
                     /translation="MNPASVLAPVLTVAAKEFRDGLRNRWVAAIAIVCALLAVGLATF
                     GAAASGQVGFTGLATTIASLASLAVFLIPLIALLLAYDSIVGEDEQGTLLLLLTYPLS
                     RLQLLAGKFLGHGAILAVSTTLGFGLAGLVIGIGSDKVATGPLVAALGLFIVSATLLG
                     WAFVGIAYVISAGASEKSRAAGLALVVWFAFVLVFDLGLLGFLVATGGAIGRDWLPWL
                     LLLNPADVFRLANLAGFDANATAGPVSIGAGFPVPLLLAVQGAWVLAPFALAVWRFRR
                     R"
     gene            complement(3592..4503)
                     /locus_tag="PK144_07185"
     CDS             complement(3592..4503)
                     /locus_tag="PK144_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65548.1"
                     /translation="MDIDRASVRYGRHCALDGLTLRLEAGEVLGLLGHNGAGKTTTMK
                     LILGLLAPQAGQVRVFGEDPHGPAAPRLRLALGYLPENVSFYDNLSGREVLDFFARMK
                     RAPRTQATELLERVGLAAAAGRRVKTYSKGMRQRLGLAQALLGSPRLLLLDEPTVGLD
                     PLATREFYARVDELRRTGTTIILCSHVLPGVERHIDRALILGQGRVLAAGSLDALREQ
                     AGLPLLIHVRGNPGPHALAALPAGSRTRMLADGRFEIALAAHAKLDALRGLLTDPAVH
                     DLDIEPPSLETLYAHFGSAVAGEGESA"
     assembly_gap    4527..4626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4655..6058)
                     /locus_tag="PK144_07190"
     CDS             complement(4655..6058)
                     /locus_tag="PK144_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNF65549.1"
                     /translation="MKRSADPLWLLAVLVCAGVLLAIGDALAATATATGRRVSPADGP
                     LQAAIDAAAPGEVLRLAPGVYAGGVKIDRPLTLAGEPGAVIDGGGQGDVVRVTAPDVT
                     LSGLGLRNSGRELGDMNSGVFLQKGADRARVEGCTIEAVAFGIWLDALDGAQILNNRI
                     TGDAALRSQDRGNGIHFFSTRHSLAAGNEVGEARDGIYIDTSDDNELRDNFLHDLRYG
                     IHYMYANRNALLGNRTTRTRTGYALMSSKNLKVIGNRSERDENYGIALNALNSSEIRD
                     NVVIDVQRGRANPSGARDAHLPAGASSGPGGAEGKALFVYNSLYNDIRGNVFAGAEIG
                     IHLTAGSEDNRIHGNAFVANRTQVRYVAVKTQEWSVDGRGNWWSDYLGWDRNDDGLGD
                     TPYEPVDGVDRLLWQYPAARVLLASPAVETLRWVQRQFPVLRPQGVRDSHPLMNPPAA
                     AIPRVASAAPGAHEATR"
     gene            complement(6128..8056)
                     /gene="nosZ"
                     /locus_tag="PK144_07195"
     CDS             complement(6128..8056)
                     /gene="nosZ"
                     /locus_tag="PK144_07195"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HNF65550.1"
                     /translation="MSEQHDPQGHGETPAGSSAAHGLTRRGFLGAAAAVGAAGATGVA
                     GALLTAQSRPAGAATAAEDPSLKAHVAPGELDEYYGFWSGGHSGEVRVLGIPSMRELM
                     RIPVFNTCSATGWGLTNESKHVLGDGARFHNGDAHHPHVSMTDGRYDGKYLFINDKAN
                     TRVARIRLDIFKCDRITSIPNVSAIHGLRLQKVPHTKYVFANAEMPIPQPNDGRDLDN
                     AQNYWTMFNAIDAESMEVAWQVIVDGNLDNTDADYTGRYAASTCYNSERGVHLGDMMR
                     NERDWVVVYNIPAIEAAIQAGKATTIGDSKVPVLDGRKAANLGITVYIPVPKNPHGLN
                     TSPDGKYFIANGKLSPTVSIIDIGKLDDLFAGKIEPRKAIAAEVELGLGPLHTTFDGR
                     GNAYTTLFLDSQVCKWNIADAIRAWNGEKVDYIRQKLDVSYQPGHNHASLTESREADG
                     KWLAVLCKFSKDRFLNTGPLKPENDQLIDISGEEMKLVHDGPTFAEPHDCIMVRRDQL
                     RPVDVWKRDDPFFAITRERAKKDGITLETDNKVIRDGRQVRVYMTSAAPTYGIQEFTV
                     KQGDEVTVTITNLDTVSDLTHGFCVVNHGVSMEIGPQQTASISFVADRPGVHWYYCNW
                     FCHAMHMEMVGRMLVEKA"
     gene            complement(8258..10525)
                     /locus_tag="PK144_07200"
     CDS             complement(8258..10525)
                     /locus_tag="PK144_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF65551.1"
                     /translation="MSFAEFRLLCLPVLRCLSALLLLVACGVAPAAPAAAPWELGRGW
                     FPGADRVAPADGTPPAAAVYRGSELLGYLFQTDQVERIPAYSGKPVNTLVGIDRSGRI
                     AGAQVLEHHEPILLVGIPETRLGEFIAQYVGKRVTDRVKVGAANREGYVGVDAITGAT
                     VTVMVINEGIMRSAHRVAVARGLIGEAAAVQRAPARVKADVYAPADWQQLSGDGSVRT
                     LRLTRADVQRAFDNTAAAGVERPTPEQLDEPFVELAWAYLNPPTIGRSLLGDAAYARL
                     MAGLAPGEHAIAVLGNGYSFKGSGYVRGGIFDRVQLRQNDQAISFRDSDFQRLSDVYA
                     PGMPRFREMVIFFIREHYHFDPGTPWQLELLVRRQTGPLDSVFTSFSGGYETPARYLE
                     LPAAPLPAPAASGSSTAGAPQAAVTATADATALAPMEPIWKAVWRERGFRIAVLGAGL
                     TLLTLILLFQDVLVTRPRLLPWLRNGFLLWTLLFIGWYGLAQLSVVNVLTFTNALIAG
                     FRWDTFLIDPMMFILWGFVAATLLLWGRGVYCGWLCPFGALQELVNELALKLGIRQWR
                     LPFAVHERLWAVKYLILLGLFAVSLQSLGEAEQLAEIEPFKTAITLRFVREWGFVAWA
                     LGLVLVSAVNRKFYCRYLCPLGAALAIPARLRLFDWLKRRRECGKPCQICAEECEVQA
                     IHPTGQINANECHYCLDCQVTYFDDGKCPPLVDKRRKRDKAAALAAGREVGREAGAPA
                     AAFDGPRRFVRPPAP"
     gene            10965..11804
                     /locus_tag="PK144_07205"
     CDS             10965..11804
                     /locus_tag="PK144_07205"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNF65552.1"
                     /translation="MSAAPEFAQPASLPPTSEDYHALTIRSVHWWSPTLFSLVLDRPE
                     SFRFISGQFARLGLPVEGEWVWRAYSICSSVFDEHLEFLSIVVPNGPFTQALKHVQAG
                     DTLYVDKRNFGTLTTDRFRGQKDLWMLATGTGLAPFMSILRDPYTWDTFEHVVLVHSV
                     RDGNELAYLEWLENFNEDEIFGEYAERFRYVKVVTREAVRGAFDKRIPDLVESGLLEQ
                     EVGWEYSPERSAVMLCGNPGMLRQMKTVLQGRGLTVNKKSSPGNILWENGFLPEDNKH
                     ADL"
BASE COUNT         1719 a         4178 c         4106 g         1718 t
ORIGIN      
        1 gcttccacgg cggcagccgg cggcgcagcc cgcgccgggc gctgcatggc gtggccgcgt
       61 ggctcgcaac cctcggctgc gcgctgccgc ttgcgatcgg cttcgtgctg ccggccgggc
      121 ggctgctcgc gctcgcgcac gccgatggcg atgcgcagct cggtccgcgc ttcctgacgc
      181 tggccggcaa cagcctggcg atctcggcgc tgaccgccgc gctcgccacg ctgctcgcgc
      241 tcgcgctcgc ctacggcgcc cggctcgcgc gcggcccggc gcgccgggcc gccgggctgc
      301 tgaaccgcgg catcgccctc ggttacgcgg tgccgggctc ggtgatcgcg gtcggcatcc
      361 tgatcccggt gacacggctc gatcatgcca tcgccgcgct ctggcagtcg ctcggccagc
      421 cgcatccggg cctgctgctg accggcgggc tcgcggcgct ggtctacggc tatctggtgc
      481 gtttcctgag cgtcgcggtg cagacggtcg acacgggcct gtcgcgcatc accgtcagca
      541 tggacgaggc cgcgcgcagc ctcgggcgcg ggcgcggcgc gctgctgcgc gaggtgcacc
      601 tgccgctgct gcgcggcagc ctcgcgagcg ccgcgctgct ggtgttcgtc gacacgatga
      661 aggaactgcc ggcgacgctg gtgatgcgcc cgttcaactt cgacacgctg gcgacccagg
      721 tctacacgct ggcagcggac gaacgcctcg ccgaggcctc gaccgcggcg cttgcgatcg
      781 tcgcggtcgg cgtgctgccg ctgatcctgc tgtcacggca catcgcgcag ggaacgcagc
      841 cgcaggacag ctgaatcctg ccccggcgcc ggcaacgggg atggccagca gccgggggac
      901 ggatgcatgc tgcaacgcat cacctgcttg cggagttcac cggcatggcc cgacgcatcc
      961 tgatcctcga cggtcacccg gaccgcgatc cggcaccgca cttcctgccg gccctgctcg
     1021 atgcctatgc cgatggcgcg cgcgcggccg ggcacgaagt cgagcggctg cgcgtcgcca
     1081 ccctcgattt cccgtggctg cgcagcggcg gcgactggga gccggcgaac acgccggccg
     1141 atgtcacgcg cctgcaggcg gcgctcacgg ccgccgatca ctggctgctc gcgtatccgc
     1201 tgtggttcgg caacccgccg gcgctgctgc tcggcctgct cgaacacgcg ctgcgccccg
     1261 gcttcgcctt cacgccgccc ggccccgacg cgccgccgaa accgctgctc ggcgggcgca
     1321 gcgcgcacgt gctggtgacg atgaacatgc cggccttcgc ctaccgctgg tacttcggcg
     1381 cccacggcgt gaagacgctg cgccggcacc tgctcggcac cgcgggcttc cgcccggtgc
     1441 gcacgacgct ggtcggcagc gtgctgacga tcggcaccgg gggccgcgaa cgctggctgc
     1501 agcggctgcg ggcgatgggc acggagggcc ggtgacgccg cccgattgcc ctgggcccct
     1561 accggcgcgc cggcgaggcc ggcatgctgt cggccccgac cgccgaggcc acgaccgctg
     1621 ccgccatgcg ccctgcatcg accgccctgc tgcttctcgc cgccctcgcc gccctgcccg
     1681 gctgctcgac cgcgcggctg ccaccgcccg aggacctcgc cggcgtgccc ggctactacg
     1741 cgagcggccg gcgcggcgcc gtgtactacg acgtcgcgat ctacctcgac gccgacaacc
     1801 gcggtgccgt ggtcatggac ggcggctgcg agggaccgcc gcgcatcctg ccgcagacgg
     1861 tcgtgcgctc cgagggttac gtcagcttcc aggccatggg ctgccgtgac ccgcgctatg
     1921 ccggcgtcga ggtcacgcag gccgaactcg ccgacggcaa ggttctcggg gccgagctgt
     1981 acctgctgcg ccggcgcgac agcgtgctcg aacgcgggcc gtcgatgacg ctgtcgccgt
     2041 aaggccgcac aaccttccgt gccggctccg gccccgctcc tgcggccgga tcacccgcct
     2101 gcccggtgcc ccgctcagcc cggctccagc ccgccggcca ccagcgccac ggtcacgtcg
     2161 aggaaggcga gcacccgccc gccctcgcgc gccgcgaagg cctcggcggc cggacgttcg
     2221 cggaagctcg cgagcgtcgg ccccatcgcg ccgagccggc gatgcccgat cacgtagaag
     2281 gcgctgcgcg cgtcggtgaa ggccgcatcg tcgggctgct cccagccggt cgcaccgaga
     2341 tcatgcaccc acagcgcctg cacgctggcg ccgacctcgg gctgcagcac ccaggcgaag
     2401 aaatcgcgcg tcgagcagaa cttcagcgcg gcctcgcgcc cgctgacgtg ggcctcgccc
     2461 ttcggccccg gaaaaccgcg gatctgcatg ccgcagaccg cgcactcgtc ggcggcggcg
     2521 atcggcaccg gccgcagcgc gacggccggc ggcctgtcct cgccgcagcc ggcgagcagc
     2581 gaggaaaacg cggatgtgaa cgtgaaggcg ccgagaccga gcaggacgtg gcggcggctg
     2641 ggcatgtgcg gaacctctcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 ntcagcgtcg ccggaagcgc cagacggcga gcgcgaacgg cgcgagcacc cacgcgccct
     2821 gcacggccag cagcagcggc acggggaagc cggcaccgat cgacaccggg ccggccgtgg
     2881 cgttcgcgtc gaagccggcg agattggcga gccggaacac gtcggccggg ttcagcagca
     2941 gcagccaggg cagccagtcg cgcccgatcg cgccgccggt cgcgaccagg aagccgagca
     3001 ggccgaggtc gaacaccagc acgaacgcga accagacgac cagcgcgagc ccggcggcgc
     3061 gggatttctc gctggcaccg gcgctgatca cataggcgat gccgacgaaa gcccagccga
     3121 gcagcgtcgc gctgacgatg aacagcccga gtgccgccac cagcggcccg gtcgcgacct
     3181 tgtccgagcc gatgccgatg acgaggccgg cgagcccgaa accgagcgtc gtcgatacgg
     3241 cgaggatcgc gccgtgaccg aggaacttgc cggcgagcag ctgcagccgc gacagcggat
     3301 aggtcagcag cagcagcagc gtgccctgtt cgtcctcgcc gacaatgctg tcgtaggcga
     3361 gcagcagcgc gatcagcggg atcaggaata ccgcgaggct cgccagactc gcgatcgtgg
     3421 tcgccagccc cgtgaagccg acctgccccg aggccgcagc cccgaaggtc gccagcccca
     3481 cggcgagcag cgcgcagacg atcgcgatcg ccgccaccca gcggttgcgc aggccgtcgc
     3541 ggaactcctt ggcggcgacg gtcagcaccg gcgcgagcac cgatgcggga ttcatgcgga
     3601 ttctccttca ccggcgacgg cgctgccgaa gtgggcgtac agggtttcga gcgacggcgg
     3661 ctcgatgtcg agatcgtgca cggccggatc ggtcagcagg ccgcgcagcg cgtcgagctt
     3721 ggcatgcgcg gccagcgcga tctcgaagcg gccatcggcg agcatgcgcg tgcggctgcc
     3781 ggccggcagt gcggcgagcg cgtgcggtcc ggggttgccg cgcacgtgga tcagcagcgg
     3841 cagcccggcc tgctcgcgca gcgcatcgag gctgccggcg gcgagcacgc ggccctgacc
     3901 gaggatcagc gcgcggtcga tgtggcgctc gacgccgggc agcacgtgcg agcagagaat
     3961 gatggtcgtg ccggtgcggc gcagttcgtc gacgcgcgcg tagaactcgc gcgtcgccag
     4021 cggatcgagg ccgacggtcg gctcatcgag cagcagcagc cgcgggctgc cgagcagcgc
     4081 ctgcgcgagt ccgaggcgct ggcgcatgcc tttggagtac gtcttcacgc gccgcccggc
     4141 cgcggcggcc agcccgacgc gttcgagcag ctccgtcgcc tgggtgcgcg gggcgcgctt
     4201 catgcgcgcg aagaagtcca gtacctcgcg accgctcagg ttgtcgtaga agctgacgtt
     4261 ctcgggcaga tagccgagcg cgagccgcag ccgcggcgca gccggaccgt gcgggtcctc
     4321 gccgaacacg cgcacctgcc cggcctgcgg agccagcagg ccgaggatca gcttcatcgt
     4381 cgtggtcttg ccggcgccgt tgtgaccgag caggccgagc acctcgccgg cttcgagcct
     4441 gagcgtcagg ccatcgagcg cgcagtggcg accgtagcgg acgctggccc ggtcgatgtc
     4501 gatgacggat tcatgccggc ttgcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnngccg gcagcagagg ggtggaaagc ttcatcatcg ggtcgcctcg tgcgcaccgg
     4681 gggcggcgct cgcgacgcgg gggatcgcgg cggccggcgg gttcatcagc gggtggctgt
     4741 cgcgcacgcc ctgcggacgc agcaccggga actggcgctg cacccagcgc agcgtctcga
     4801 ccgcgggact cgcgagcagc acgcgcgccg ccgggtactg ccagagcagg cggtcgacgc
     4861 cgtcgacggg ctcgtacggc gtgtcgccga ggccgtcgtc gttcctgtcc cagccgaggt
     4921 agtcgctcca ccagttgccg cggccgtcga ccgaccattc ctgggtcttc accgcgacgt
     4981 acctgacctg cgtgcggttg gcgacgaagg cgttgccgtg gatgcggttg tcctcggaac
     5041 cggcggtcag gtgaatgccg atttcggcac cggcgaagac gttgccgcgg atgtcgttgt
     5101 agagcgagtt gtagacgaac agcgccttgc cctcggcccc gcccggaccg ctgctggcgc
     5161 cggccggcag gtgcgcatcg cgtgcgccgc tcgggttggc acgaccgcgc tgcacgtcga
     5221 tgacaacgtt gtcgcggatc tcggagctgt tcagcgcgtt cagcgcgatg ccgtagttct
     5281 cgtcgcgctc ggagcggttg ccgatgacct tcaggttctt cgaagacatc agcgcgtagc
     5341 cggtgcgcgt gcgcgtcgtg cggttgccga gcagcgcgtt gcggttggcg tacatgtaat
     5401 gaatgccgta gcgcaggtcg tgcaggaagt tgtcgcgcag ctcgttgtcg tcgctggtgt
     5461 cgatgtagat gccgtcgcgc gcctcgccga cctcgttgcc ggcggcgagc gaatggcggg
     5521 tgctgaagaa gtggatgccg ttgccgcgat cctgcgagcg cagcgcggca tcgccggtga
     5581 tgcggttgtt caggatctgc gcgccgtcga gcgcatcgag ccagatgccg aaggcgaccg
     5641 cctcgatcgt gcagccctcg acgcgcgccc ggtcggcgcc cttctgcagg aacacgcccg
     5701 agttcatgtc gccgagttcg cgcccggagt tgcgcagccc gaggccgctc agcgtcacat
     5761 cgggcgccgt gacccgcacg acatcgccct gcccgccgcc gtcgatgacg gcgccgggct
     5821 cgccggcgag cgtcagcggc cggtcgatct tcacgccgcc ggcgtagacg ccgggcgcaa
     5881 gccgcagcac ctcaccgggc gccgccgcgt cgatcgccgc ctgcagcggc ccgtcggccg
     5941 gcgacacgcg tcgtccggtg gcggtggcgg tggcggcgag tgcatcgccg atcgccagca
     6001 gcacgccggc acagaccagc acggccagca gccacagggg gtccgcagat cgcttcatgt
     6061 ccatccctcc cgccggctgc cgcagccgca cggcgtgcgg ctgcggaccg gctgcggcgc
     6121 gcctgcctca ggccttctcg accagcatcc gcccgaccat ctccatgtgc atcgcgtggc
     6181 agaaccagtt gcagtagtac cagtgcacgc cgggccggtc ggcgacgaag ctgatcgagg
     6241 cggtctgctg cgggccgatc tccatcgaca cgccgtggtt gacgacgcag aagccgtgcg
     6301 tgagatccga gacggtgtcg aggttggtga tcgtcaccgt cacctcgtcg ccctgcttca
     6361 ccgtgaactc ctggatgccg taggtcggcg ccgccgaggt catgtagacg cgcacctgcc
     6421 ggccgtcgcg gatgaccttg ttgtcggttt cgagcgtgat gccgtccttc ttcgcacgct
     6481 cgcgcgtgat ggcgaagaac ggatcgtcgc gcttccagac atcgacgggc cggagctggt
     6541 cgcggcgcac catgatgcag tcgtgcggct cggcgaaggt cgggccgtcg tgcacgagct
     6601 tcatctcctc gccggagatg tcgatcagct ggtcgttctc gggcttcagc ggcccggtgt
     6661 tcaggaagcg atccttcgag aacttgcaga gcaccgccag ccacttgccg tcggcctcgc
     6721 gcgattcggt caggctggcg tggttgtggc cgggctgata gctcacgtcg agcttctggc
     6781 ggatgtagtc gaccttctcg ccgttccagg cgcggatcgc gtcggcgatg ttccacttgc
     6841 agacctggct gtcgaggaac agcgtcgtgt aggcgttgcc gcggccgtcg aaggtcgtgt
     6901 gcagcgggcc gagtccgagt tcgacctcgg cggcaatggc cttgcgcggc tcgatcttgc
     6961 cggcgaacag atcatcgagc ttgccgatgt cgatgatcga cacggtcggc gacagcttgc
     7021 cgttggcgat gaagtacttg ccgtccggcg aggtgttgag gccgtgcggg ttcttcggca
     7081 ccgggatgta aaccgtgatg ccgaggttcg cggccttgcg cccgtcgagc accggcacct
     7141 tcgaatcgcc gatcgtcgtc gccttgccgg cctggatcgc cgcctcgatc gccgggatgt
     7201 tgtagacgac cacccagtcg cgctcgttgc gcatcatgtc gccgagatgc acgccgcgct
     7261 cggagttgta acaggtcgag gctgcgtacc tgccggtgta gtcggcatcg gtgttgtcga
     7321 ggttgccatc gacgatgacc tgccaggcga cctccatgct ctcggcgtcg atggcgttga
     7381 acatggtcca gtagttctgc gcgttgtcga gatcacgccc gtcgttcggc tgcgggatcg
     7441 gcatctcggc gttcgcgaac acgtacttcg tgtgcggcac cttctgcagg cgcaggccgt
     7501 ggatcgccga gacgttcggg atcgacgtga tcctgtcgca cttgaagatg tcgaggcgga
     7561 tgcgcgcgac gcgggtgttg gccttgtcgt tgatgaacag gtacttgccg tcgtagcggc
     7621 cgtcggtcat cgacacgtgc ggatgatgcg cgtcgccgtt gtggaagcgc gcgccgtcgc
     7681 cgagcacgtg tttgctctcg ttggtcaggc cccagcccgt ggccgagcag gtgttgaaca
     7741 ccgggatgcg catcagctcg cgcatcgacg ggatgccgag cacgcgcacc tcgccggaat
     7801 gcccgccgga ccagaagccg tagtactcgt cgagctcgcc cggcgcgacg tgcgccttca
     7861 gcgacgggtc ctcggcagcg gtcgccgccc cggccgggcg cgactgcgcc gtcagcagcg
     7921 caccggcaac gccggtcgcg ccggcggcgc cgacggccgc cgcggcgccg agaaagccgc
     7981 ggcgcgtcag accgtgcgca gcactgctgc cggccggcgt ttccccgtgg ccctgcgggt
     8041 cgtgttgctc gctcatggtg atccgtcctc agaagattgc actgtgaatg ccgccggcga
     8101 cccgcgggcg cagaccgccc ccgggttcgt tccgaacggc ggattcggcg ccgcgtccgc
     8161 cgggactccg gcagcgatgc gacggcgagg gaaaaaagcg cgggtgctga cccgccggcg
     8221 aagggcgtga agcaggcatg gcggcgggcc cggtggctca gggcgccggc gggcgcacga
     8281 agcggcgcgg cccgtcgaag gccgcggccg gggcgccggc ctcgcgaccg acctcacggc
     8341 cggcggcgag tgccgcggcc ttgtcgcgct tcctgcgctt gtcgacgagt ggcggacact
     8401 tgccgtcgtc gaagtacgtg acctggcagt cgaggcagta atggcactcg ttggcgttga
     8461 tctgcccggt cgggtggatc gcctgcacct cgcattcctc ggcgcagatc tggcacggct
     8521 tgccgcactc gcgccggcgc ttcagccagt cgaacagccg caggcgcgcc gggatcgcca
     8581 gcgccgcgcc gagcgggcac aggtagcggc agtagaactt gcggttgacg gccgagacga
     8641 ggaccagacc cagcgcccag gcgacgaagc cccactcgcg cacgaagcgc agcgtgatcg
     8701 cggtcttgaa cggctcgatc tcggcgagct gctcggcctc gccgagcgac tgcagcgaca
     8761 ccgcgaacag cccgagcagg atcaggtact tcaccgccca cagccgctcg tgcaccgcaa
     8821 acggcagccg ccactggcgg atgccgagct tcagcgccag ctcgttgacg agttcctgca
     8881 gcgcgccgaa ggggcagagc cagccgcagt agacgccgcg tccccacagc agcagcgtcg
     8941 cggcgacgaa gccccagagg atgaacatca tcggatcgat gagaaacgta tcccaccgga
     9001 agccggcgat cagcgcgttc gtgaaggtca gcacgttgac gaccgacagc tgcgcgagcc
     9061 cgtaccagcc gatgaacagc agcgtccaga gcaggaagcc gttgcgaagc cacggcagca
     9121 gccgcggccg cgtcaccagc acgtcctgga acagcaggat cagcgtcagc agcgtcaggc
     9181 ccgcgccgag caccgcgatc cggaaaccgc gctcgcgcca caccgccttc cagatcggct
     9241 ccatcggcgc cagcgcggtc gcatccgccg tggccgtcac cgcggcctgc ggcgcgcctg
     9301 cggtgctgct gccggaggcg gccggcgccg gcagcggtgc ggcgggcagc tccagatagc
     9361 gcgccggcgt ctcgtagccg ccgctgaagc tcgtgaacac cgaatcgagc ggcccggtct
     9421 ggcggcgcac cagcagttcg agctgccacg gcgtgcccgg atcgaagtgg taatgctcgc
     9481 ggatgaagaa gatcaccatc tcgcgaaagc gcggcatgcc gggcgcgtag acatcgctca
     9541 ggcgctggaa gtcgctgtcg cggaagctga tcgcctggtc gttctggcgc agctgcacgc
     9601 gatcgaagat gccgccgcgc acatagcccg agcccttgaa cgaatagccg ttgcccagca
     9661 ccgcgatcgc gtgctcgccg ggcgcaagcc ccgccatcag ccgtgcatac gcggcatcgc
     9721 cgagcaggct gcgcccgatg gtcggcgggt tcaggtaggc ccaggccagt tcgacgaagg
     9781 gctcgtcgag ctgctccggc gtcgggcgct cgacgccggc ggcggcggtg ttgtcgaagg
     9841 cgcgctgcac gtcggcgcgc gtcagccgga gcgtgcgcac cgagccgtcg ccgctgagct
     9901 gctgccagtc ggccggcgcg tagacatcgg ccttcacacg cgccggtgcg cgctgcacgg
     9961 ccgccgcctc gccgatcagg ccgcgcgcga ccgcgacccg gtgcgccgag cgcatgatgc
    10021 cctcgttgat gaccatgacg gtcacggtcg cgccggtgat cgcatcgacg ccgacgtagc
    10081 cctcgcggtt ggcggcgccg accttgacgc gatcagtcac gcgcttgccg acgtactgcg
    10141 cgatgaactc gccgagccgg gtctcgggga tgccgaccag caggatcggc tcgtggtgtt
    10201 cgagcacctg cgcaccggcg atgcggccgc tgcggtcgat gccgaccagc gtgttgaccg
    10261 gcttgcccga ataggccggg atgcgctcga cctgatcggt ctggaacagg tagccgagca
    10321 gctcgctgcc gcggtacacg gccgcagccg gcggcgtgcc atcggcgggc gcaacacgat
    10381 cggcgcccgg gaaccagccg cggccgagct cccacggcgc cgccgccggt gcggccggcg
    10441 cgacgccgca agccacgagc agcagcagcg cactcaaaca gcgcaggacc ggcaggcaca
    10501 gcaggcggaa ttcagcgaag gacatggcat ctgcaacggc caccgctcgc gcggagggca
    10561 gccgtgcccg gtcagggtgc ggcgatctgc cgcaggtcgg ggcacaccgt agccccctga
    10621 ccggccagta tcttgatgat catcaagccc atgaccattg tgccggccac ttacccccga
    10681 gggggcagcc acccgtactt gccctgcgcc ggccggctac ccgcaccgat caggtgcatc
    10741 tgccgctacc tctgccatgc ctgcgcatcg caaccggccc ggcgctgccg gcgcctgccg
    10801 cttcggagcg catcggccgg gaggcagggc ctcatataag aagattgaat aaaaaaatcc
    10861 attcagattc tttccagcaa tttaatatcc gacttatcct cccggacaat tttcgccccc
    10921 cgagggcagg caccgccctt ccgtcagccg agaacctgca atccatgtcc gcagcacccg
    10981 agttcgccca gcccgcctcc ctgcccccga ccagtgagga ctaccacgcc ctgaccatcc
    11041 gcagcgtgca ctggtggtca ccgacgctgt tctcgctggt gctcgaccgt ccggagtcct
    11101 tccgcttcat ctccggccag ttcgcccgtc tcggcctgcc ggtcgaaggc gaatgggtct
    11161 ggcgggcgta ctcgatctgt tcgtcggtct ttgacgagca cctggaattc ctgtcgatcg
    11221 tcgtgccgaa cggcccgttc acgcaggcgc tgaagcacgt gcaggccggc gacacgctct
    11281 acgtcgacaa gcgcaacttc ggcacgctga ccaccgaccg cttccgcggc cagaaggatc
    11341 tctggatgct cgcgaccggc accggcctgg cgccgttcat gtcgatcctg cgcgacccct
    11401 acacctggga caccttcgag cacgtcgtgc tggtgcacag cgtgcgcgac ggcaacgagc
    11461 tcgcgtatct cgaatggctc gaaaacttca atgaggacga gatcttcggc gagtacgccg
    11521 agcgtttccg ctacgtgaag gtcgtcacgc gcgaggccgt gcgcggcgcc ttcgacaagc
    11581 gcatccccga cctcgtcgag tccggcctgc tggagcagga agtcggctgg gagtactcgc
    11641 ccgagcgctc ggcggtcatg ctctgcggca accccggcat gctgcgccag atgaagacgg
    11701 tgctgcaggg ccgcggcctg accgtcaaca agaagtccag ccccggcaac atcctctggg
    11761 aaaacggctt cctgcccgag gacaacaagc acgccgatct ctgatccggc ggcgtttccg
    11821 ctttcccccg acggccggca cgcgccctgc gtgccggccg tcgcgtttgc ggccgcccgg
    11881 cagcaagccc cgcccccgcg cccccgcccg gctcccgcca g
//
LOCUS       DAOQIE010000087        11798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2671_length_11798_cov_7.684323, whole genome shotgun sequence.
ACCESSION   DAOQIE010000087 DAOQIE010000000
VERSION     DAOQIE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_11798_cov_7.684323"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK144_07210"
     CDS             <1..493
                     /locus_tag="PK144_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF65553.1"
                     /translation="ADLYYRLNVLALRLPPLRERPADLAPLAQHFAQQLAGELGLPAP
                     LLDAAALAALAAHDWPGNVRELRNLIERALLLGCPPAECLPQTPAIAAAAASAASASA
                     VVTAATGVPEPAPLALDAVEKRHILQVLDACGGNKSVAARRLGIARKTLDRKLAAWQG
                     RPS"
     gene            534..2603
                     /locus_tag="PK144_07215"
     CDS             534..2603
                     /locus_tag="PK144_07215"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNF65554.1"
                     /translation="MPVRLFGRYGHGIRHRLLLLVLLPSVLLLPMLLLTTLAWGERFS
                     QRQSLARVATDLSVAESVFARMQRDTLDALHRLADSWALRTALAGGTPVPMALLERDV
                     GGDGFGYVRLLDAGGRPRDGGPPPPPSKLLDAARAGQAASGIERFEAAALDAARDGLA
                     ALARVEHAGTQEPADERGLVVRAAVPVLDEQGAVLALLDGGVLLNRNYRPVDEIRDLV
                     YGPGTLLPGAIGTVTLFLDDLRVSTNVPLHAGERALGTRVSPEVRRQVLEHGERWLGR
                     AFVVNAWYLSAYAPLLDVHGERIGMLYTGFLEAPYRQAWDDAAGALIGACLLADALLG
                     ALALWLARSLFRPLERLLAVTRAVRSGATGARALPGPGRDELAELAREFDAMLDRLAQ
                     HEHELETAAQALERTVAERTEDLRRQNARLAETVAELAEARQRLLTTEQLAALGTLAA
                     GVAHEINNPLAVLLGNLDVMVAELGEAAAPVQVEIDLAVAQVERIRRIVRGLLDYARP
                     QRQPLGGCVADLPALFEETLLLAGHSVQGGGVMIQRDWQARQAVRIGAPELQQVLLNL
                     IVNALQALAGRGRLWLTTADKQGRVVLVVADDGPGIEARDLPRVFDPFFTTRTSGTGL
                     GLAVSFAIVRRHGGEITIGTRPGGGCLVTVCLPALEAAGCKSGADSPSCALASPSPSL
                     SCRESDR"
     gene            2600..6013
                     /locus_tag="PK144_07220"
     CDS             2600..6013
                     /locus_tag="PK144_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65555.1"
                     /translation="MKPLSLSPFALALVWQTLHARLSLRLALLAVAVFLTGTLADAPG
                     SASPSMAVWCLDAGLLALALRARRRHVPAYLLVSFLAAVAGRLAAGDGVWFALGFAVA
                     SAACVVVPTLILRSSQPAPVLLVPGGNQRPALHVAAAVIAGVVIAAAFGSAVASTRPG
                     SVYTERWLEWFAGDVLGMVGLLPLGLTLTRARLERLRQPGCSVDLLVALLLTGASTLL
                     GLRFAPFPFVVIGLPLLLVALRLPLLGTTLVVACETLLYAGLAAGGVAPGPYVDALSS
                     NAHIFNAATLLAPLLVAGLREARAADAMRLQQLAARGASVLDGVGQGTWEWHPASGEA
                     HFSPGWHALTGIAPGAGTAGIEHWIHHVHPEDVGRVLGGLEAHIDGEGSEFDCAHRIG
                     DGEGGWRWVRARGRIVERTADAAPLALFGLLTDIDAEVQAEAERIALAQRLSTALDAG
                     GIALWEWDLERGCLLWSEHLYELLDWRPAFEQARAAQWLAIVHPPDRRRVRLAWARAA
                     SAGEKFTIEFRVCPPGSGTTRTLRCTGEPGGDDRHTLRGAAIDVTEARQLASALADEK
                     ARLQVTLYGIGDAVIATDLQARVNFLNPAAERMLDLPGHEAIGLPLATLLRLVDERSG
                     EPIADPVVACLRSGERTELPDGSTLLGHDGRRFDLAGSAAPLRAGDGSLRGAVLVCQD
                     VTARRAFQRELHHQAAHDHLTGLLNRRAFERALDELVTALHRGPAREHALCFVDLDRF
                     KAVNDTAGHAAGDALLGRVAALLRAGVRGTDVIARLGGDEFALILVDCPQAQAVKVAD
                     KLVAAVRSLRFVWEGQVHEIGASIGLVPVTAGSGNVATLTSQADVACYAAKGLGRNRV
                     AVYSPDNEAIAREHREISAADGIRQALEAGRFSLYAQPIVAVGTPESEQYFELLLRMV
                     DGEGRLVPPGAFLPAAERYELMPALDRWAIGHALHTLGPTIAALPALMVSLNLSAGSI
                     NDPSFRPWLEAALAQTPVPVARIGFEFAESVAVSNLIAAGDLIQWLRARGCRVALDDF
                     GTGLSSFSYLKHFPVDFVKIDGSFIAALGHGAVDRAIVESLNDIAHRLGVRTIAEGVE
                     SSETLGLLQQLGIDYAQGMALGAPVALGEQLAALRELDGGGRPKLKLVR"
     gene            complement(6123..7082)
                     /locus_tag="PK144_07225"
     CDS             complement(6123..7082)
                     /locus_tag="PK144_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNF65556.1"
                     /translation="MLSRVAQNLYWLARYVERAEDTARLIGVTSNLLLDLPRTAGAAE
                     WEPLIYITGSHEGFREHFTRPDEVSIVSFLTGDLRNSGSILSSLTYARESLRTTRDVM
                     PREAWEELNRIYLFVRDHISDGVSPRGRDYFLKQVVSGCQRFNGYLEGCMSYTPARDF
                     VLLGRYLERADMTTRILDVRSGNLLTQSGDLSPYASIQWMSVLRSLSGYQMYRQAVRS
                     RVRGPDVLAFLLQDTRFPRTVRHCLDRIADCLRELPQPETALAQIARACAKTESADVE
                     ALVTTGLHEFIDELQVELGEVHNAINAAYFTYHEKETPPAAAA"
     gene            complement(7086..8543)
                     /locus_tag="PK144_07230"
     CDS             complement(7086..8543)
                     /locus_tag="PK144_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNF65557.1"
                     /translation="MSMLDWQQYPAGAFYDELVAPGGTPRQAARTLCEHLGKLSDAEL
                     TERRTAAELAISVMGITFTVYSEGNMIDRAWPFDIVPRIIPLTEWTRIEAGLKQRVQA
                     LNLFIDDLYHEQKVIRDGILPAEILAQSVNFRPQCVGITPPHRVWAHICGSDLVRDKD
                     GTVYVLEDNLRVPSGVSYMLENRVITKRVLPELFKTSRILPVDDYPAQLFEMLSSMSP
                     RPGEDPRIVVLTPGIYNSAYFEHAYLAQQMGVQLVEGPDLFVDDDDCCYMRTIYGPQR
                     VDVIYRRIDDLFLDPEAFIPDSMLGVRGLLRSWRAGKVGLANAPGAGVADDKVVYAFV
                     PELIKYYLDQDAILSNVPSYLCMRDKDRDYVLANLDKLVVKPANESGGYGMLVGPHST
                     PEKRAQFAELIKADPRNYMAQPTLAISTSPTLCDGNAAEPRHVDLRPFILSGPKPYVT
                     TGGLTRVALVKGSLVVNSSQGGGSKDTWIVDTEET"
     gene            9059..>11798
                     /locus_tag="PK144_07235"
     CDS             9059..>11798
                     /locus_tag="PK144_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF65558.1"
                     /translation="MTIRVALNHKTSYHFDRPVSLSPHIVRLRPAPHARTPIPAYSLK
                     INPQPHFINWQQDPFGNFQARLVFPEKVREFDVEVDLVAEMTVINPFDFFVEEYADNF
                     PFEYTPELKKELAPYLEIRERGPLLLDWLRDVSLDPVNTTSFLVALNQRLQRDVGYVI
                     RMEPGVQAAEETLAKGTGSCRDSAWLMVQIARHLGLAARFVSGYLIQLTADVKALDGP
                     SGTEVDFTDLHAWTEIFVPGAGWIGLDPTSGLLAGEGHIPLAATPDPTGAAPITGYTD
                     KCEVEFGFAMSVTRIHEDPRVTKPYTDEQWTEIEALGHAVDARLDAGDVRMTMGGEPT
                     FVSIDDMDGAEWNTAAMGPTKRKLAGTLLKRLRRQFSPGGLLHYTQGKWYPGESLPRW
                     ALNCFWRNDGEPIWRDEALIADDTAPGRVTAADAQRFGAELSARIGVSPKCLIPGYED
                     AYYYLWYERTLPVNIDPLDYDLKDGEERRRLAQLLERDLGSVTGYALPLRWNYARGSW
                     QSGQWPLRREHLYLIPGDSPMGLRLPLTALPWVSDTDLPVEQSLFEFRKPLRDLHGEI
                     ARRYSALLDGRDVSGHHHEGVNQDWQEQPEHDDDDTIVHTALCIEPREGHLYVFMPPL
                     GYLEHYLELIAAIEHTAAALSIPVFIEGYAPPSDPRLKKFAVTPDPGVIEVNIHPAGS
                     WDELVHNTHVLYEEARQSRLGTEKFMLDGRHTGTGGGNHVTLGGATPADSPFLRRPDL
                     LRSLVTYWQHHPGLSYLFSGMFVGPTSQAPRVDEARNDSLYELEIAFQQMPPGETPQP
                     WLVDRLLRNLLVDLSGNTHRAEFCIDKLYSPDSATGRLGLVEMRAFEMPPHARMSLMQ
                     MLLLRTLVARFWERPYPNRELVRWGTQIHDRFMLPHYVWEDFKDVVEDLNRAGYPFE"
BASE COUNT         1642 a         4139 c         4148 g         1869 t
ORIGIN      
        1 cgccgatctg tactaccgtc tgaacgtgct ggcgctgcgt ctgccgccgc tgcgcgagcg
       61 cccggccgac ctcgcgccgc ttgcgcagca tttcgcgcag cagctcgccg gcgaactcgg
      121 cctgccggcg ccgctgctcg atgccgcggc gctggcggca ctggccgcgc acgactggcc
      181 cggcaacgtc cgtgagctgc gcaacctcat cgaacgcgcg ctgctgctcg gctgcccgcc
      241 ggccgagtgc ctgccgcaga cgccggccat cgccgccgcc gctgcaagcg cggccagcgc
      301 gagcgcggtc gtgacggccg cgacaggcgt gccggagccg gcgccgcttg cgctcgatgc
      361 cgtcgagaag cgccacatcc tgcaggtgct cgatgcctgc ggcggcaaca agtccgtggc
      421 cgcgcgccgc ctcggcatcg cccgcaagac cctggatcgc aagctcgccg cctggcaggg
      481 gcgtccatcc tgagcgcggg tgtgccgctg ccgatgcccc ggccatccgc ctgatgccgg
      541 tacgcctgtt cggccgctac ggccacggca tccgccatcg cctgctgctg ctggtgctgc
      601 tgccgagcgt gctgctgctg ccgatgctgc tgctgacgac gctcgcctgg ggcgagcgct
      661 tcagccagcg tcagtcgctg gcgcgcgtcg cgaccgacct gtcggtggcc gagagcgtct
      721 tcgcgcgcat gcagcgcgac acgctcgatg cgctgcaccg gctcgcggat tcctgggcgc
      781 tgcgcacggc gcttgccggc ggtacgccgg tgcccatggc gctgctggag cgcgatgtcg
      841 gcggcgatgg cttcggctac gtgcgcctgc tcgatgccgg cggcaggccg cgcgacggcg
      901 gtccgccgcc gccgccgtcg aaactgctcg atgccgcgcg cgccggacag gcggcaagcg
      961 gcatcgagcg cttcgaggcc gcggctctcg acgccgcgcg cgacggtctg gccgcgctcg
     1021 cgcgcgtcga gcacgccggc acccaggagc cggccgatga gcgcgggctg gtcgtgcgcg
     1081 ccgcggtgcc ggtgctcgat gagcagggcg cggtgctggc gctgctcgat ggcggcgtgc
     1141 tgctgaaccg caactaccgg ccggtcgacg agatccgcga tctcgtctac ggccccggca
     1201 cgctgctgcc gggcgcgatc ggcacggtga cgctgtttct cgatgacctg cgcgtgtcga
     1261 ccaacgtgcc gctgcacgcc ggcgagcggg cgctcggcac ccgcgtctcg cccgaggtgc
     1321 gccgccaggt gctggagcac ggcgagcgct ggctcgggcg cgccttcgtc gtcaacgcct
     1381 ggtatctgtc ggcctacgcg ccgctgctcg acgtccacgg cgagcgcatc ggcatgctct
     1441 acaccggctt tctcgaagct ccgtaccggc aggcctggga cgatgccgct ggcgcgctga
     1501 tcggcgcctg cctgctcgcc gatgcgctgc tcggggcgct ggcgctgtgg ctggcgcgtt
     1561 cgctgttccg gccactggag cgcctgctcg ccgtgacccg cgccgtgcgc agcggtgcga
     1621 ccggtgcccg cgccctgccc ggacccgggc gtgacgaact cgccgagctg gcccgcgagt
     1681 tcgatgcgat gctcgatcgg ctcgcgcagc acgagcacga actcgaaacc gcggcacagg
     1741 cgctggagcg cacggtcgcc gaacgcaccg aggacctgcg ccgccagaac gcccggctgg
     1801 ccgagaccgt cgccgagctg gccgaggcgc gccagcgcct gctgaccacc gagcagctcg
     1861 ccgcgctcgg cacgctcgcc gccggcgtcg cgcacgagat caacaatccg ctggccgtgc
     1921 tgctcggcaa cctcgatgtc atggtcgccg aactcggcga ggccgcggcc ccggtgcagg
     1981 tcgagatcga cctcgccgtc gcgcaggtcg agcgcatccg ccgcatcgtg cgcggcctgc
     2041 tcgattacgc ccggccgcag cgccagccgc tcggcggctg tgttgccgat ctgccggcgc
     2101 tgttcgagga aaccctgctg ctcgccggcc acagcgtgca gggcggcggg gtcatgatcc
     2161 agcgtgactg gcaggcccgt caggccgtgc gcatcggcgc acccgagctg cagcaagtgc
     2221 tgctgaacct gatcgtcaac gcgctgcagg cgctcgccgg ccgcggccgg ctgtggctga
     2281 cgacggccga caagcagggc cgcgtcgtgc tggtcgtggc cgacgacgga ccgggcatcg
     2341 aggcccggga tctgccgcgg gtgttcgatc cgttcttcac gacccgcacc agcggcaccg
     2401 ggctcggact ggccgtcagt ttcgcgatcg tgcgtcgcca tggcggcgag atcacgatcg
     2461 gcacgcgacc gggcgggggc tgtcttgtga cagtctgttt gccggcactc gaggctgccg
     2521 gctgcaagag cggcgccgat tcgccatcat gcgccctggc gtcgccatcg ccgtccctgt
     2581 cctgccgcga atcagaccga tgaagccgtt gtccctgtcg cccttcgcgc tcgcccttgt
     2641 ctggcagacg ctgcatgcgc ggctgtccct gcggctggcg ctgctggcag tcgcggtgtt
     2701 cctgaccggc actcttgccg atgcgcccgg cagtgcaagt ccgtcgatgg cggtctggtg
     2761 cctggatgca gggctgctgg cgctggcgct gcgcgcccgg cgcaggcacg tgcctgcgta
     2821 tctgctcgtc agcttcctgg ccgcggtggc gggccggctc gccgccggtg acggcgtctg
     2881 gttcgcgctc ggctttgcgg tggccagcgc cgcctgcgtc gtcgtgccga cgctgatcct
     2941 gcgcagctcc cagccggccc cggtgctgct cgtgccgggc ggcaaccagc gcccggcgct
     3001 gcacgtcgcc gcagcggtga tcgccggcgt cgtgatcgcg gcggccttcg gttcggcggt
     3061 cgccagcacg cggcccggct ccgtctacac cgaacgctgg ctggaatggt ttgccggtga
     3121 cgtgctcggc atggtcgggc tgctgccgct cgggctgacg ctgacacgcg cgcgtctgga
     3181 gcggctgcgc cagcccggct gcagtgtgga tctgctggtg gcgctgctgc tgaccggcgc
     3241 cagcacgctg ctcggcctgc gcttcgcgcc gtttccgttc gtcgtcatcg gcctgccgct
     3301 gctgctggtg gcgctgcgcc tgccgctgct cggcacgacg ctggtcgtgg cctgcgagac
     3361 gctgctgtac gccggtctgg cggccggcgg cgtggcaccc ggcccctatg tcgatgcgct
     3421 gagcagcaac gcccacatct tcaacgccgc gacgctgctg gcgccgctgc tggtcgcggg
     3481 cctgcgcgag gcccgggccg cggatgccat gcggctgcag cagctcgctg cccgcggcgc
     3541 cagcgtgctc gacggcgtcg ggcagggaac ctgggaatgg catcccgcca gcggcgaggc
     3601 acatttctcg cccggctggc atgcgctgac cggcatcgca ccgggcgccg gaacggccgg
     3661 catcgaacac tggatccacc acgtccatcc cgaggatgtc ggtcgcgtgc tcggcggcct
     3721 cgaagcccac atcgatggcg agggcagcga gttcgactgc gcccaccgca tcggcgacgg
     3781 cgagggcggc tggcgctggg tgcgcgcgcg cgggcgcatc gtcgagcgca cggccgatgc
     3841 cgcgccgctg gcgctgttcg gcctgctgac cgacatcgat gccgaggtgc aggccgaggc
     3901 cgagcgcatc gcgctcgcgc agcgcctgag caccgcactc gatgccggcg gcatcgcgct
     3961 gtgggaatgg gatctcgagc gcggctgcct gctgtggtcc gaacaccttt acgagctgct
     4021 cgactggcga ccggccttcg agcaggcgcg ggccgcgcag tggctggcca tcgtgcatcc
     4081 gcctgaccgc cggcgcgttc gcctggcctg ggcgcgcgcc gccagtgccg gcgagaagtt
     4141 cacgatcgaa ttccgcgtat gtccgccggg ttcgggtacg acgcgcacgc tgcgctgtac
     4201 cggcgagccc ggcggcgatg accggcacac gctgcgcggt gccgcgatcg acgtgaccga
     4261 agcacgccag ctcgcctcgg ccctcgccga cgagaaggcc aggctgcagg tgacgctgta
     4321 cggcatcggc gatgccgtga tcgccaccga cctgcaggcg cgcgtgaact tcctgaatcc
     4381 ggccgccgaa cgcatgctcg acctgccggg gcacgaggcg atcggcctgc cgctcgcgac
     4441 gctgctgcgc ctcgtcgacg agcgcagcgg cgagccgatc gcggacccgg tcgtggcctg
     4501 cctgcgcagc ggcgagcgca ccgagctgcc cgacggctcg acgctgctcg ggcacgacgg
     4561 ccggcgcttc gacctcgccg gctcggccgc gccgctgcgt gccggcgacg gcagcctgcg
     4621 cggcgcggtg ctggtctgtc aggacgtgac cgcccgccgg gccttccagc gcgagctgca
     4681 tcaccaggca gcccacgatc acctgaccgg cctgctgaac cggcgtgcct tcgaacgcgc
     4741 gctcgatgag ctggtcacgg cgctgcaccg cgggccggcg cgcgagcacg cgctctgctt
     4801 cgtcgatctc gaccgcttca aggccgtcaa cgacaccgcc ggccacgcgg ccggcgatgc
     4861 gctgctcggg cgggtcgcgg cgctgctgcg cgccggggtg cgcggcaccg acgtgatcgc
     4921 acgtctgggc ggcgacgagt tcgcgctgat cctcgtcgac tgcccgcagg cgcaggcggt
     4981 caaagtcgcc gacaagctgg ttgccgccgt gcgcagcctg cgcttcgtct gggaagggca
     5041 ggtccacgag atcggcgcga gcatcggtct ggtgccggtc acggccggct ccggcaacgt
     5101 cgccaccctc accagtcagg ccgatgtggc ctgctatgcg gccaagggcc tcgggcgcaa
     5161 ccgcgtcgcc gtctattccc cggacaacga ggccatcgcc cgcgagcacc gcgagatctc
     5221 ggcagcggac ggcatccgcc aggcgctgga ggccgggcgc ttcagcctgt acgcgcagcc
     5281 gatcgtcgcc gtcggcacgc cggaatccga acagtatttc gagctgctgc tgcgcatggt
     5341 cgacggtgag ggccggctgg tgccgcccgg ggccttcctg ccggccgccg aacgctacga
     5401 gctgatgccg gcgctggatc gctgggcgat cggtcacgcg ctgcacacgc tcgggccgac
     5461 catcgccgcc ctgcccgcgc tcatggtgtc gctgaacctg tcggccggct cgatcaacga
     5521 tccgtcgttc cggccctggc tggaggcggc gctggcgcag acgccggtgc cggtggcacg
     5581 catcgggttc gagttcgccg agtcggtcgc cgtcagcaac ctgatcgccg ccggcgacct
     5641 gatccagtgg ctgcgcgccc gcggctgccg ggtggcgctc gacgatttcg gcaccggcct
     5701 cagttccttc agctatctca agcacttccc ggtcgatttc gtgaagatcg acggcagctt
     5761 catcgccgcg ctcggccacg gtgcggtcga tcgcgccatc gtcgaatcgc tgaacgacat
     5821 cgcgcaccgg ctcggcgtgc gcacgatcgc cgaaggcgtc gagtcgtccg agacgctggg
     5881 tctgctgcag cagctcggca tcgattatgc gcagggcatg gcgctcggtg cgccggtggc
     5941 gctcggcgag cagctcgccg ccctgcgcga actcgacggc ggcggccggc cgaagctgaa
     6001 gctggtgcgc tgagccggcc ggcgcccggc ctgccggcgc gcccggtcgg cactcatata
     6061 gatgaaagag gccggtgccc cggagggcac cggcctcgcg tgcgtcggcg ctgcgctgcg
     6121 cttcaggcgg cggcggccgg cggcgtttcc ttctcgtggt aggtgaagta cgcggcgttg
     6181 atggcgttgt ggacttcgcc gagttcgacc tgcagctcgt cgatgaactc gtgcaggccg
     6241 gtggtcacga gcgcctcgac atcggccgat tcggtctttg cgcaggcccg tgcgatctgt
     6301 gcgagcgcgg tttcgggctg cggcagttcg cgcaggcagt cggcgatgcg gtccaggcag
     6361 tggcgcacgg tgcgcgggaa gcgcgtgtcc tgcagcagga aggcgagcac gtccggaccg
     6421 cgcacgcgcg agcgcacggc ctgacggtac atctggtagc ccgacagcga acgcagcacg
     6481 ctcatccact ggatgctcgc gtacggcgac aggtcgccgc tctgcgtcag caggttgccc
     6541 gagcgcacgt cgaggatgcg cgtggtcatg tcggcgcgtt cgaggtagcg cccgagcagc
     6601 acgaagtcgc gcgccggcgt gtagctcatg cagccttcga ggtagccgtt gaagcgctgg
     6661 caaccgctga cgacctgctt caggaagtag tcgcggccgc gcgggctgac gccgtcggag
     6721 atgtggtcgc gcacgaacag atagatgcgg ttgagctcct cccaggcctc gcgcggcatc
     6781 acgtcacggg tcgtgcgcag gctctcgcgg gcgtaggtca gcgagctcag gatcgagccg
     6841 ctgttgcgca ggtcaccggt caggaagctg acgatgctga cttcgtccgg gcgggtgaag
     6901 tgctcgcgga aaccctcgtg gctgccggtg atgtagatca gcggctccca ctcggcggca
     6961 ccggccgtac gcggcagatc gagcagcagg ttgctggtca cgccgatcag gcgcgcggtg
     7021 tcctccgcgc gctcgacgta gcgggccagc cagtagaggt tctgggcgac gcgcgaaagc
     7081 atggatcagg tctcctcggt atcgacgatc caggtgtcct tgctgccgcc accctgggag
     7141 gaattgacga cgagcgaacc cttgaccagc gcgacgcggg tcaggccgcc ggtggtcacg
     7201 tagggcttgg gaccggacag gatgaacggg cgcaggtcga cgtggcgcgg ctcggcggcg
     7261 ttgccgtcgc agagcgtcgg gctggtcgag atcgccagcg tcggctgcgc catgtagttg
     7321 cgcggatcgg ccttgatcag ctcggcgaac tgcgcgcgct tctcgggcgt cgagtgcggt
     7381 ccgaccagca tgccgtagcc gccggactcg ttggccggct tgaccacgag cttgtcgagg
     7441 ttggcgagca cgtagtcgcg gtccttgtcg cgcatgcaca ggtagctcgg cacgttcgac
     7501 aggatcgcgt cctgatcgag gtagtacttg atcagctccg gcacgaaggc atagacgacc
     7561 ttgtcatcgg ccacgccggc gcccggggcg ttggcgagcc cgaccttgcc ggcacgccac
     7621 gagcgcagca ggccgcgcac gccgagcatc gagtcgggga tgaaggcctc gggatcgagg
     7681 aacaggtcgt cgatgcgccg gtagatcacg tcgacgcgct gcggcccgta gatcgtgcgc
     7741 atgtagcagc agtcgtcgtc atcgacgaac agatccggcc cctcgaccag ctgcacgccc
     7801 atctgctgcg cgaggtaggc gtgttcgaaa taggccgagt tgtagatgcc gggggtcagc
     7861 acgacgatgc gcggatcctc gccggggcgc ggcgacatcg agctcagcat ctcgaagagc
     7921 tgcgccgggt agtcatcgac cggcaggatg cggctggtct tgaacagctc gggcagcacg
     7981 cgcttggtga tcacgcggtt ctcgagcatg tacgagacgc cagagggcac gcgcaggttg
     8041 tcctccagca cgtagacggt gccgtccttg tcgcgtacga ggtcggagcc gcagatatgc
     8101 gcccagaccc ggtgcggcgg cgtgatgccg acgcactgcg gccggaagtt caccgactgc
     8161 gcgaggatct cggccggcag gatgccgtcc cggatgacct tctgctcgtg atacaggtcg
     8221 tcgatgaaca ggttcagggc ctgcacgcgc tgtttcaggc cggcctcgat gcgcgtccac
     8281 tcggtcagcg ggatgatcct cggcacgatg tcgaacggcc aggcccggtc gatcatgttg
     8341 ccttcggaat agaccgtgaa cgtgatgccc atgacgctga tcgccagttc ggcagcggtg
     8401 cgccgctcgg tcagctcggc atcggagagc ttgccgaggt gctcgcagag cgtgcgcgcc
     8461 gcctgccgcg gcgtaccgcc gggcgcgacc agttcgtcat agaaggcccc tgcggggtat
     8521 tgctgccagt caagcatgct catgatggtg tgattccttg tgccgggcgc cgttgaatgc
     8581 ggcgccgcct gcgacgcgcc cccgaactgc ctctccccgg ggggcgcaac gactacttca
     8641 acagcaagcc ccgtaccact cgccgggctc cgctgtccgg gatcgcggca gacggtgccg
     8701 gcggctcagc cctgccggtc ccgccggacg tgcggcaggg gccggacgct cgcagcgcca
     8761 tgatggggca ggggtttaga ccaaaaggcc ggtgaggcgg ctggttttga cggataagcg
     8821 gctcggacat ggcgttgtgc atttgattgg tgcgtcctgc acggcaaatt gcacttcatt
     8881 ggtgcgtgag ggcaaggact gtcggatcac cggcggactt cgcagaggca ttcgcctggc
     8941 tttggccccg cctgtctgcc ggatgctccg gaccgcgtaa ttgtcacgga ctggaacgcc
     9001 ggttgctagt acacttcgcg cccggccgtt gccgtgagag cccagggaac ttcccccgat
     9061 gacaatccgc gttgcactga accacaagac ttcttatcac ttcgatcgcc cggtatcgct
     9121 gtcgccgcac atcgtcaggc tgcgtcccgc gccgcatgct cgcacaccga tcccggcgta
     9181 ttcgctgaag atcaatccgc agccgcactt catcaactgg cagcaggacc cgttcggcaa
     9241 tttccaggcc cgtctggtat tcccggaaaa agtcagggaa ttcgatgtcg aggtcgatct
     9301 ggtcgcggaa atgaccgtga tcaatccgtt cgatttcttc gtcgaggaat acgccgacaa
     9361 cttcccgttc gaatacacgc ccgaactgaa gaaggaactg gcgccgtacc tggagattcg
     9421 cgagcgcggt ccgctgctgc tcgactggct gcgcgatgtc tcgctcgatc cggtcaacac
     9481 cacgagcttc ctcgtggcgc tgaaccagcg cctgcagcgc gacgtcggct acgtgatccg
     9541 catggagccc ggcgtgcagg ccgccgagga gaccctggcc aaggggaccg gttcgtgccg
     9601 tgactcggcc tggctgatgg tgcagatcgc ccggcatctc ggtctcgccg cgcgcttcgt
     9661 gtccggctac ctgatccagc tgaccgccga cgtgaaggcc ctcgacggcc cgagcggcac
     9721 cgaggtcgac ttcaccgacc tgcacgcctg gaccgagatc ttcgtgccgg gcgccggctg
     9781 gatcggcctc gatccgacct ccggcctgct cgccggcgaa ggccacatcc cgctcgcggc
     9841 cacgcccgac ccgaccggcg cggcgccgat caccggctac accgacaagt gcgaggtcga
     9901 gttcggcttc gcgatgagcg tcacgcgcat ccacgaggac ccgcgcgtca ccaagcccta
     9961 caccgatgag cagtggaccg agatcgaggc tctcggccac gccgtcgatg cgcgtctcga
    10021 cgccggcgac gtgcgcatga ccatgggcgg cgagccgacc ttcgtgtcga tcgacgacat
    10081 ggacggcgcc gagtggaaca ccgccgcgat gggcccgacc aagcgcaagc tggccggcac
    10141 gctgctcaag cgcctgcgcc ggcagttctc gccgggcggc ctgctgcact acacgcaggg
    10201 caagtggtat ccgggcgagt cgctgccgcg ctgggcgctg aactgcttct ggcgcaacga
    10261 cggcgagccg atctggcgcg acgaggcgct gatcgccgat gacaccgcgc cgggccgggt
    10321 cacggccgca gacgcacagc gcttcggcgc cgagctgtcg gcgcgcatcg gcgtgtcgcc
    10381 gaagtgcctg attcccggct acgaggatgc ctactactac ctctggtacg aacgcacgct
    10441 gccggtcaac atcgatccgc tcgactacga cctgaaggat ggcgaggagc gccgccgcct
    10501 ggcgcagctg ctggagcgcg acctcggctc ggtgaccggc tacgcgctgc cgctgcgctg
    10561 gaactacgcg cgcggcagct ggcagagcgg gcagtggccg ctgcgccgcg agcacctgta
    10621 cctgatcccc ggcgattcgc cgatgggcct gcgcctgccg ctgaccgcgc tgccgtgggt
    10681 cagcgacacc gacctgccgg tcgagcagtc gctgttcgag ttcaggaagc cgctgcgcga
    10741 cctgcacggc gagatcgcac ggcgctacag cgcgctgctc gacggccgcg acgtgtcggg
    10801 tcatcaccac gagggcgtca accaggactg gcaggaacag cccgagcacg acgatgacga
    10861 cacgatcgtg cacaccgcgc tgtgcatcga gccgcgtgaa ggccacctgt acgtgttcat
    10921 gccgccgctc ggctatctgg agcactacct cgaattgatc gccgcgatcg agcacacggc
    10981 cgcggccctg agcatcccgg tgttcatcga gggctacgcc ccgccgtccg atccgcgcct
    11041 gaagaagttc gcggtgaccc cggacccggg cgtcatcgag gtcaacatcc acccggccgg
    11101 ctcctgggac gaactcgtcc acaacacgca cgtgctgtac gaggaagccc gccagtcgcg
    11161 cctcggcacc gagaagttca tgctcgacgg ccgccacacc ggcaccggcg gcggcaacca
    11221 cgtcacgctc ggcggcgcca ccccggccga ttcgccgttc ctgcgccggc ccgacctgct
    11281 gcgcagcctg gtcacgtact ggcagcatca ccccggcctg tcctacctgt tctcgggcat
    11341 gttcgtcggc ccgaccagtc aggcgccgcg cgtcgacgag gcgcgcaacg acagcctgta
    11401 tgagctggag atcgccttcc agcagatgcc gcccggtgaa acgccacaac cctggctggt
    11461 ggatcggctg ctgcgcaacc tgctggtcga cctgagcggc aacacccatc gcgccgagtt
    11521 ctgcatcgac aagctgtatt cgcccgattc cgccaccggc cggctgggtc tggtggaaat
    11581 gcgcgctttt gaaatgccgc cgcacgcgcg tatgagcctg atgcagatgc tgctgctgcg
    11641 taccctggtg gcgcggttct gggagcggcc ctatccgaat cgcgagctgg tgcgctgggg
    11701 cacgcagatt cacgaccgct tcatgctgcc gcactacgtc tgggaggatt tcaaggacgt
    11761 ggtggaggat ttgaaccgtg ccggctaccc cttcgaga
//
LOCUS       DAOQIE010000088        11726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2699_length_11726_cov_7.384543, whole genome shotgun sequence.
ACCESSION   DAOQIE010000088 DAOQIE010000000
VERSION     DAOQIE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2699_length_11726_cov_7.384543"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /gene="rsxC"
                     /locus_tag="PK144_07240"
     CDS             complement(<1..2063)
                     /gene="rsxC"
                     /locus_tag="PK144_07240"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNF65559.1"
                     /translation="MSLLNTLGRLWRFHGGVHLPEHKQESTGLPITVLAAAGELVLPL
                     QQHIGAPARACVQVGQPVAKGEVIARADGYVSVPLHAPTSGTVLAIEPRPIAHPSGID
                     APAIVLAADGRDAWHPDLPLPLEEGLALDPAVLRTRVRESGIVGLGGAAFPTAVKLNL
                     HGRIQVLILNAAECEPYLTCDDVLMRERAREVIGGLLLMRRALDAGRCLIGIEDNKPE
                     AAAALEAALHAFGGAATGVELRVIPTLYPSGGEQQLIRVLTGVEVARSRRPVDSGILC
                     QNVGTAAAVHAAVTTGRPLISRIVTITGAGVASPRNLEVAFGTPVRALVEACGGYTER
                     AQRLILGGPMMGLAVAGDEVPVTKGMGCVLVAAADELAPPAPTLPCIRCGSCMDACPA
                     NLLPQQLYWFARGKEFDKAQATRLFDCIECGACAAVCPSRLPLVQYYRYAKSEIGAAE
                     REKQKSEQARRRHEFRTQRLEREEAERLARQQAKKARATAPAANEASASAAAAPDDDP
                     KKAAIAAALARAQEKKAAKVAETGTGTAPPATASPAAPAAGLDEAQLAKLAAARARAE
                     AKKAAQAAAAAPEAMPAAAEAASAPAAPAPAPAMSEEQLAKLAAARARAEAKKAAQAA
                     PAAPEAAPGSAGETPAPAAPALSEEQLAKLAAARARAEAKKAAQAAPAAREATPVAAA
                     ADPAPA"
     gene            complement(2225..2776)
                     /gene="rsxB"
                     /locus_tag="PK144_07245"
     CDS             complement(2225..2776)
                     /gene="rsxB"
                     /locus_tag="PK144_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNF65560.1"
                     /translation="MFTAIVALSGLATLFGCLLGYASVRFRVEGDPLAERLDAVLPQT
                     QCGQCGFAGCKPYAEALAKGEAAIDLCAPGGQPGVVAIAGVLGVEPTSAANDGEPVRM
                     HAVIHEEGCVGCTVCIKACPVDAILGATKLMHTVISAECTGCGLCVGPCPVNAIELMP
                     VEVTLSTWKWTLPAALPTLRTAA"
     gene            complement(2782..3363)
                     /gene="rsxA"
                     /locus_tag="PK144_07250"
     CDS             complement(2782..3363)
                     /gene="rsxA"
                     /locus_tag="PK144_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNF65561.1"
                     /translation="MKDYVLILIGTILVNNFVLVKFLGLCPFMGVSKKVETALGMGMA
                     TTFVLTLSSACTWFMYSAVLVPLGLEYLNTIAFILVIAVVVQFTEMVVHKTSPVLYQV
                     LGIYLPLITVNCAVLGVALLNVQAHHDFLGATLYGFGAAAGFSLVLVLFAALRERIAV
                     ADVPLPFRGPAIALVTAGLMSLAFLGFTGFVRG"
     gene            complement(3755..4672)
                     /gene="argF"
                     /locus_tag="PK144_07255"
     CDS             complement(3755..4672)
                     /gene="argF"
                     /locus_tag="PK144_07255"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNF65562.1"
                     /translation="MTIRHFTTLADCTPAELAYLIRRAGVLKARHRAGERHAPLPGRV
                     LAQIYEKASTRTRVSFEAGIAQLGGSAIFLSPRDAQLGRGESIEDTARVISRMVDLVM
                     IRAFRHETVTRFAAASQVPVINGLTDHAHPCQLLADIQTWSELRGPVAGRTVAWVGDG
                     NNMCNSYLTAAELFDFRLRIACPEGYEPDAALLARHADRVELLRDPLAAAAGADLVTT
                     DVWASMGHEDEMEKRAAAFAGYCVDAAVMAAAAPDAIFLHCLPAHRGEEVAAEVIDGS
                     QSAVWEEAENRMHAQKALMEFLLLGEQAD"
     gene            5264..5977
                     /locus_tag="PK144_07260"
     CDS             5264..5977
                     /locus_tag="PK144_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF65563.1"
                     /translation="MTLLKTRFLLAPLAALLLGATAPALAAVLSQQLGDIDFPSDPGL
                     PNADPDAINHRIGTVTFTNASGGDPAPFNQFIGSDTTTDFTASWTFSAYGGAILDPVS
                     AATIQIGLYEGDSAATGNQVAAFTVDGVDLTAALNALLEATPAYTGTEVHYTLSLSAA
                     AIAELLDGSATFSLTLQGPGASVIGDTTNNGAGIDFSILSISTDNGGTPPTGVPEPGT
                     LALAASGFGLLAARRRRRG"
     gene            6116..6619
                     /locus_tag="PK144_07265"
     CDS             6116..6619
                     /locus_tag="PK144_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNF65564.1"
                     /translation="MDPFLGEIRLFGGNFAPQDWAFCNGQLLPIRQNQALFSLLGTQY
                     GGDGRTTFALPDLRGRCAPHPGMGLVQGQSGGSETVTLQSQHLPAHGHLPRCAAQGGT
                     DKPSGGYWAGDGSGNTAAYAPASDGTPLAASALAPAGGGQPHENMQPFLALNFIIALS
                     GIYPSRS"
     gene            6634..7152
                     /locus_tag="PK144_07270"
     CDS             6634..7152
                     /locus_tag="PK144_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNF65565.1"
                     /translation="MSEPFLGEIRPFAFTFVPRGWASCAGQLLSIAQNSALFSILGTT
                     YGGDGRTTFALPDLRDRVPIAAGSGPGLTPRSPGEAGGSASVTLTVAQLPAHTHTPVA
                     QSQPGTTGSAVDDVWARDAGGGKLYSVQGGAQMAADALAASGSGAAHDNLQPGLAIQF
                     CIAIEGIYPSHG"
     gene            7180..7680
                     /locus_tag="PK144_07275"
     CDS             7180..7680
                     /locus_tag="PK144_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNF65566.1"
                     /translation="MDPFIGELKLVGFPYPPQGWMACEGQLLSIAQNQALFALIGTLY
                     GGDGQQTFALPDLRGRVPLGTGPTQPPGQWSGEDSVTLTAAQMPAHTHAVQCHAEPGT
                     QAEPINGFWSVDTGGNALYAATADATMSAAAISTAGSSQPHPNRQPYQCLYWIIATEG
                     VFPSRA"
     gene            7677..8174
                     /locus_tag="PK144_07280"
     CDS             7677..8174
                     /locus_tag="PK144_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF65567.1"
                     /translation="MTGPVPLPARRPETPADADFLSGLFRAHRAEAFAVPGLPPADLD
                     ALLRLQVQAQVAHYRRQWPQARAEIVLDAGAAPIGRLLSARSVRELRLIDIALLPACC
                     GCGLGTRLIAALIDEAGAAGLALTAHVARDNPARRLYARLGFREHDDGGPYLAIRHAV
                     PGREA"
     gene            complement(8188..10026)
                     /locus_tag="PK144_07285"
     CDS             complement(8188..10026)
                     /locus_tag="PK144_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNF65568.1"
                     /translation="MSEPRYSVGIDLGTTHCVLAAADLSLTDGETVALALEPVAQLTA
                     PGAVEERTLLPSFLYLPHPDELAPADLALPWDTPEGFLVGELARSLGAKTPLRLVASA
                     KSWLCHPGIDRRGPVLPVEAPAEVARISPFDATVRYLEHLADAWDARHPDAPLDAQAL
                     TITVPASFDPAARELSAEAAQAAGLEDFILLEEPQAALYSWIEATAGGWRKQARVGDV
                     ILVADVGGGTADFSLIAVGEREGGLDFTRIAVGDHILLGGDNMDLALAHVLRAKLAAA
                     GTQLEPWQVQGLVHGCRAAKEQILGDAAADAVPVVVPSRGSRLIGGTLRTELTRVEVE
                     ATLVEGFFPRVGAEARPLARARTGLTRLGLPYAQDAGVTRHLAAFLARQLDAAPGCGI
                     ELPAGASFLHPTAVLFNGGVFKAAALEARVLDVLNAWLAAEGAPPARRLPGTQLDHAV
                     ARGAAYYGCVRRGRGVRIRGGTARSYYVGIESSMPAVPGIEPPVEALCVAPFGMEEGT
                     EAELPAQEVGLVVGEPVRFRFFGSPVRRDDRVGDVLEVWGGEELEELPEIEATLPAEG
                     RTPGEVVPVRLHAAVTELGTLRLEAVSRDASERWKVELEVRQADEA"
     gene            complement(10106..10711)
                     /locus_tag="PK144_07290"
     CDS             complement(10106..10711)
                     /locus_tag="PK144_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNF65569.1"
                     /translation="MSEPTPSIFERVFLAFSAFGRTLASGRFAAGVARLRHGDAGERA
                     AAALPVVMPEPLAAVPPPPPAPVAPPVVLREVTPDAALQLLGLLQQEGRLIDFLQEDL
                     GAAGDADIGAAARIVHEGCRKVLRTHFELAPVRSEREGSRLTLPAGFDAGAVRLTGRV
                     LGQPPFTGTLVHCGWRASAVKLPKLAEGHDVHVVAPAEVEL"
     gene            complement(10824..>11726)
                     /locus_tag="PK144_07295"
     CDS             complement(10824..>11726)
                     /locus_tag="PK144_07295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNF65570.1"
                     /translation="RMLEVVRLGTSERRWRLFFRAAWLIVVLLVLAQWRAGSDMTAAT
                     LGGGRHAAKVSVDGLIAPGTDASAERVLHGLRTAFADREAAGIILDINSPGGSPVQAG
                     QVYAELRRLRAQHPDKPVYAVIGDVGASGAYYIAAGAQEIYADRASLVGSIGVRMDGF
                     GFVEAMQRLGIERRLLTSGANKGLLDPFSPVQPAQLAHVQTLLDAIHQQFIAAVREGR
                     GERLHETPEIFSGLFWTGEQAQALGLVDGYGSVESIARDKLGTTQIRDHTQQEKPFER
                     LFGQVESTLAGALTRAFGLDSPLR"
BASE COUNT         1670 a         4168 c         4282 g         1606 t
ORIGIN      
        1 gccggcgccg ggtctgccgc tgcggccacg ggggttgctt cccgtgcagc cggagcggcc
       61 tgcgcggcct tcttcgcctc ggcacgcgcg cgggcggcgg cgagcttcgc gagctgttcc
      121 tcgctcagtg ccggcgcagc cggcgccggc gtttccccgg cgctcccggg ggctgcttcg
      181 ggtgcagccg gagcggcctg cgcggccttc ttcgcttcgg cgcgggcgcg cgcggcggcg
      241 agtttcgcga gctgttcctc gctcattgcc ggagccggag ccggagcggc cggcgccgat
      301 gctgcttcgg cggcggccgg catggcttcg ggtgcagccg ctgcggcctg tgcagccttc
      361 ttcgcctcgg cccgggcgcg ggcggcagca agctttgcga gctgggcctc gtcgagcccc
      421 gcagcgggcg cggccgggct ggcggtggcc gggggcgcgg tgccggtccc ggtctccgcg
      481 accttcgcgg ccttcttctc ctgggcccgt gcgagggcgg cggcgatcgc ggccttcttc
      541 ggatcgtcgt ccggtgcggc ggctgcgctc gcgctcgcct cgttggccgc cggcgcagtg
      601 gcgcgcgcct tcttggcctg ctggcgcgcc aggcgctcgg cttcctcgcg ttcgaggcgc
      661 tgcgtgcgga actcgtggcg gcggcgcgcc tgttcggatt tctgcttctc gcgctcggcg
      721 gcgccgatct cggacttggc gtagcggtag tactgcacca gcggcagccg gctcgggcag
      781 accgccgcgc aggcaccgca ttcgatgcag tcgaacagcc gcgtggcctg cgccttgtcg
      841 aactccttgc cgcgcgcgaa ccagtacagc tgctgcggca acaggttggc cgggcaggca
      901 tccatgcacg agccgcagcg gatgcacggc agcgtcggtg ccggcggtgc cagttcgtcg
      961 gccgcggcga ccagcacgca gcccatgccc ttggtgaccg gcacctcgtc gccggcgacc
     1021 gcgagcccca tcatcggccc gccgaggatc agccgctgcg cgcgctcggt gtagccgccg
     1081 caggcctcga ccagcgcccg caccggcgtg ccgaacgcga cttcgaggtt gcgcgggctg
     1141 gcgacgccgg cgccggtgat cgtgacgatg cgcgagatca gcggccggcc ggtggtcact
     1201 gcggcgtgca cggcggcggc ggtgccgacg ttctggcaca ggatgccgga gtcgaccggc
     1261 cggcggctgc gcgcgacctc gacgccggtc agcacgcgga tcagctgctg ctcgccgccc
     1321 gagggataca gcgtcgggat cacgcgcagc tcgacgccgg tggcggcccc gccgaaggcg
     1381 tgcagcgcgg cttcgagcgc cgccgcggcc tcgggcttgt tgtcctcgat gccgatcagg
     1441 cagcgcccgg catcgagtgc gcggcgcatc agcagcaggc cgccgatgac ctcgcgggcg
     1501 cgctcgcgca tcagcacgtc gtcgcaggtg aggtagggct cgcactcggc ggcgttcagg
     1561 atcagcacct ggatgcgccc gtgcaggttc agcttcaccg cagtcgggaa ggccgcgccg
     1621 ccgaggccga cgatgccgga ttcgcgcacg cgggtccgca gcacggccgg atcgagcgcc
     1681 agaccctctt cgagcggcag cggcaggtca ggatgccagg catcgcggcc gtcggcggcg
     1741 agcacgatcg ccggcgcgtc gatgccggac gggtgtgcga tcggtcgcgg ctcgatcgcg
     1801 agcaccgtgc ccgaggtcgg cgcgtgcagc ggcacgctga cgtagccgtc ggcgcgggcg
     1861 atgacttcgc ctttggcgac cggctggccg acctgcacgc aggcgcgcgc cggtgcgccg
     1921 atgtgctgct gcagcggcag caccagttcg ccggcagcag ccagcaccgt gatcggcagg
     1981 ccggtcgatt cctgcttgtg ctcgggcaga tgcacgccgc cgtggaagcg ccacagacgg
     2041 cccagcgtgt tcagcagcga catggcgcgg acggctcgca ggcgaagaag cgggtggacg
     2101 cggggcggac cggcacggac ccgtccggtt tcatacggat cggcaaggat cgatacggaa
     2161 ccggcacgga tccgggcgga tcgctccgga tcggtggcgg agattcagcg gttcaggcgg
     2221 cggttcaggc ggccgtgcgc agcgtcggca gggccgccgg cagcgtccac ttccaggtcg
     2281 agagcgtgac ctcgaccggc atcagctcga tggcgttgac cgggcagggc ccgacgcaca
     2341 ggccgcagcc ggtgcattcg gccgagatca ccgtatgcat cagcttggtc gccccgagga
     2401 tcgcatcgac cgggcaggcc ttgatgcaca ccgtgcagcc gacgcagcct tcctcgtgga
     2461 tcacggcgtg catgcgcacc ggctcgccgt cgttggccgc cgaggtcggc tcgacgccga
     2521 gcacgccggc gatcgcgacc acgccgggct ggccgccggg cgcgcacagg tcgatcgccg
     2581 cctcgccttt cgcgagcgct tcagcgtagg gcttgcagcc ggcgaagccg cactggccgc
     2641 actgcgtctg cggcagcacg gcgtcgaggc gttcggcgag cggatcacct tcgacgcgaa
     2701 agcgcaccga ggcataaccg agcaggcatc cgaacagtgt ggcgagtccg gagagggcga
     2761 cgatggcagt gaacatggga atcaaccccg cacgaaaccg gtgaagccga ggaaggcgag
     2821 cgacatcagg cccgcggtga cgagtgcgat ggccggaccg cggaacggca gcggcacatc
     2881 ggcaacggcg atgcgttcgc gcagcgccgc aaacagcacg agcacgagcg agaagccggc
     2941 ggcggcgccg aagccgtaca gcgtcgcgcc gaggaaatcg tgatgcgcct gcacgttcag
     3001 cagcgccacg ccgagcaccg cgcagttgac cgtgatcagc ggcaggtaga tgccgagcac
     3061 ctgatagagc acggggctgg tcttgtgcac gaccatttcc gtgaactgca cgacgacggc
     3121 gatgaccagg atgaaggcga tcgtgttcag gtattcgagc ccgagcggca ccagcacggc
     3181 gctgtacatg aaccaggtgc aggccgagga cagcgtcagc acgaaggtcg tcgccatgcc
     3241 catgccgagc gcggtctcga ccttcttcga gacgcccatg aacgggcaga gaccgagaaa
     3301 cttcacgagc acgaagttgt tgacgagaat cgtgccgatc aggatcagga cgtagtcttt
     3361 catggttccg ccaggtcccc ggtcaccggt tgattgccgc cgccggtcgc agtgcgaccg
     3421 gcggtccctt caataaacca ttacggcgtc atggttttcg gccttgttcc gcctgtcttg
     3481 cagcctacgg cagggcggcg gtgccaaaac atgacgcagc gcaacagcag gcggtttcgc
     3541 gagcgatccg tgcgtggttc gccggcatga aacttgataa ggagctgcct gtcgccgtgt
     3601 ggcgcgagcc ggcgggcgaa ataccccaca aactgcacgg cccggtcggg gaccgggccg
     3661 gagcgattcg cattacggct gcaagcggct gattctagcg aagtaccggt gcggcggaca
     3721 aggcaggtgc cgccaccggt gcggcccggc ggcctcagtc ggcctgctcg ccgagcagca
     3781 ggaactccat cagcgccttc tgcgcatgca tgcgattttc cgcttcctcc cagacggcgc
     3841 tctgcgatcc gtcaatcacc tcggccgcga cttcctcgcc gcggtgcgcg ggcaggcagt
     3901 gcaggaagat cgcatccggc gcggccgcgg ccatcacggc ggcatcgacg cagtagccgg
     3961 cgaaagcggc tgcgcgtttc tccatctcgt cctcgtggcc catgctcgcc cagacgtcgg
     4021 tcgtgaccag atcggccccg gcggcggcgg cgagcggatc gcgcagcagc tcgacgcgat
     4081 cggcgtggcg cgcgagcagc gcggcatcgg gctcgtagcc ctcggggcag gcgatgcgca
     4141 gccggaagtc gaacagctcg gcggcggtga ggtagctgtt gcacatgttg ttgccgtcgc
     4201 cgacccaggc gaccgtgcgc ccggcgaccg ggccgcgcag ttcgctccag gtctggatgt
     4261 cggcgagcag ctggcaggga tgggcgtggt cggtcaggcc gttgatgacc ggcacctgcg
     4321 aggcggcggc gaagcgcgtg acggtttcgt ggcggaaggc gcggatcatg accagatcga
     4381 ccatgcgcga gatcacgcgc gcggtgtcct cgatcgattc gccgcgcccg agctgcgcat
     4441 cgcgcggcga caggaagatc gccgagccgc cgagctgcgc gatgccggcc tcgaaggaca
     4501 cgcgcgtgcg cgtcgaggct ttctcgtaga tctgtgcaag cacgcgcccg ggcagcggtg
     4561 cgtggcgttc gccggcgcga tgcctggcct tgagcacacc ggcgcgccgg atcaggtacg
     4621 cgagttcggc gggcgtacag tcggccagcg tcgtgaagtg tcgaatcgtc atctcgggca
     4681 atctcctccc cccagggagc acggcggacg cgcggagtgc gtcccgcggt ccgtgccgtc
     4741 accggccgga ccacgtacga cgccgtagcg ggccgccggg gcgctcggcg gtccggtcgg
     4801 cgaccggtcg tcgggatcgg gtcgccggct gccggtcggc ggcggcgcga tccatccaca
     4861 aagtctgctc cacgatcctg ccgggagcag cctgaaacat tttgagacag tttttgaaac
     4921 tgtccggccg tcgcgcccgg atttctggtg gtttgccggc atcgctgcgg atcgatgcag
     4981 gctaagtgcg cgtcagcagc ggaaatcggg cgcggcggaa aaacgggcga gcctaccggg
     5041 gacgcccggg atgggcaagc cgaacctgca gcaaaagccg gccggacccg ggataaatcc
     5101 ctgaaccgcc ttgcatccgg caaaagccgg atactgatgt tgcaagggtg ggcctatcct
     5161 gtggcggcgg ttgtccagag gccggtacac aaggaccgga tccatgccgg cggcagccgc
     5221 accagcggta acccagccca cacgacaagg aaatccgatc accatgaccc tcctgaaaac
     5281 ccgattcctc ctcgcgccgc tcgccgcgct gctgctgggc gccacggccc cggcgcttgc
     5341 cgccgtgctg tcgcagcagc tcggtgacat cgacttcccg agcgatcccg gtctgccgaa
     5401 cgcggatccc gatgccatca atcaccgcat cggcacggtg accttcacga atgccagcgg
     5461 aggcgatccg gcgccgttca accagttcat cggctccgac acgacgacgg atttcacggc
     5521 cagctggacc ttcagcgcct atggcggcgc gatcctcgac ccggtgagcg cggcgacgat
     5581 ccagatcggc ctctacgagg gcgattcggc cgcgaccggc aatcaggtgg ctgccttcac
     5641 ggtcgatggc gtcgacctga ccgcggcgct gaacgcgctg ctcgaagcca cgccggccta
     5701 caccggcacc gaagtccact acacgctgtc gctgtcggcg gccgcgatcg ccgaactgct
     5761 cgatggcagc gcgaccttca gcctgacgct gcagggaccg ggcgccagcg tgatcggcga
     5821 caccacgaac aacggtgccg gcatcgattt ctcgatcctg agcatcagca ccgacaacgg
     5881 gggcacgcca ccgaccggcg tgcccgagcc cggcaccctc gcgctggcgg cctcgggctt
     5941 cggcctgctc gcggcccggc gccggcggcg cggctgagtc cggcgcggcg cggcaagcac
     6001 tgcttgccgg acggcggacg gacggctgct agtacttgag ccctgcagcg tcagtcgcac
     6061 cggccccgga gccgggtgac cggcgctgtc cttccgggcc tcacggagcc gtctcatgga
     6121 tccctttctt ggagaaatcc gcctgttcgg cggtaacttc gccccgcagg actgggcgtt
     6181 ctgcaacggg cagctgctgc cgatccgcca gaaccaggcc ttgttcagcc tgctcggcac
     6241 gcagtacggc ggcgacggcc gcaccacctt cgcgctgccc gacctgcgcg ggcgctgcgc
     6301 gccgcacccg ggcatggggc tggtgcaggg ccagagcggt ggcagcgaga ccgtgacgct
     6361 gcagtcgcag cacctgccgg cccacggcca tctgccgcgc tgcgccgcgc agggcggcac
     6421 cgacaagccg tcggggggct actgggccgg ggacggcagc ggcaacacgg ccgcctatgc
     6481 cccggcgagc gacggcaccc cgctcgcggc cagcgccctg gcgcctgccg gcggcgggca
     6541 gccgcacgag aacatgcagc ccttcctcgc gctgaatttc atcatcgcgc tgagcggcat
     6601 ctatccgtcc cgcagctgat ccgggagacc ctcgtgagcg aacccttcct gggcgagatc
     6661 cgcccgtttg ccttcacgtt cgtgccgcgc ggctgggcga gctgtgccgg ccagctgctg
     6721 tcgatcgcgc agaattccgc gctgttctcg atcctcggca cgacttacgg cggcgatggc
     6781 cgcacgacgt ttgcgctgcc ggacctgcgc gaccgcgtgc cgatcgccgc cggcagcggc
     6841 ccgggcctga cgccgcgcag ccccggcgag gccggcggca gcgcgagcgt gacgctgacg
     6901 gtcgcgcagc tgccggcgca cacgcacaca cccgttgccc agagccagcc cggcaccacg
     6961 ggcagcgccg tcgacgatgt ctgggcgcgc gatgccgggg gcggcaagct ctactcggtg
     7021 cagggcggag cgcagatggc ggccgatgcg ctcgccgcaa gcggcagcgg cgcggcgcac
     7081 gacaacctgc agcccggtct ggccatccag ttctgcatcg ccatcgaagg catctacccg
     7141 tcgcacggct gagccggccc ctttccggag accccgttca tggatccgtt catcggagaa
     7201 ctcaagctgg tcggcttccc ctatccgccg cagggctgga tggcctgcga gggccagctg
     7261 ctgtcgatcg cacagaatca ggcgctgttc gcgctgatcg gcaccctcta cggcggcgac
     7321 ggccagcaga ccttcgcgct gcccgacctg cgcggacgcg tgccgctcgg caccggcccg
     7381 acgcagcccc ccggacagtg gtccggcgag gacagcgtga cgctgacggc cgcgcagatg
     7441 ccggcccaca cgcacgccgt gcagtgccac gccgagcccg gcacgcaggc ggagccgatc
     7501 aacggtttct ggtcggtcga taccggcggc aatgcgctgt acgcggcgac ggccgatgcg
     7561 acgatgtccg ccgcggccat ctcgacggcc ggcagcagcc agccgcatcc gaaccgtcag
     7621 ccgtaccagt gcctgtactg gatcatcgcg accgaaggcg tgtttccgtc gcgcgcatga
     7681 cgggccccgt gccgctgccg gcgcgccggc cggaaacccc ggccgatgcg gacttcctga
     7741 gcgggctgtt ccgcgcgcac cgggcggagg cattcgccgt gcccggcctg ccgccggcgg
     7801 acctcgatgc gctgctgcgc ctgcaggtgc aggcgcaggt cgcgcactac cgccggcaat
     7861 ggccgcaggc gcgcgccgag atcgtgctcg acgccggcgc ggccccgatc gggcggctgc
     7921 tgagcgcccg cagcgtgcgg gaactgcgcc tgatcgacat cgcgctgctg ccggcctgct
     7981 gcgggtgcgg gctcggcacg cggctgatcg cggcgctgat cgacgaggcc ggcgccgccg
     8041 gcctggcgct caccgcgcac gtcgcgcgcg acaacccggc gcgacggctg tatgcgcggc
     8101 tcggtttccg cgagcacgac gacggcgggc cgtacctcgc gatccgccac gccgtgcccg
     8161 gccgggaggc ctagaggatg tctgcgttca ggcctcgtcg gcctgacgca cctccagctc
     8221 gaccttccag cgctcgctcg catcgcgtga caccgcttcg aggcgcagcg tgccgagttc
     8281 ggtgaccgcc gcgtgcaggc gcaccggcac gacctcgccg ggcgtgcggc cctcggccgg
     8341 cagcgtcgcc tcgatctcgg gcagttcttc gagctcctcg ccgccccaga cctccagcac
     8401 gtcgccgacg cggtcatcgc ggcgcaccgg cgagccgaag aagcggaagc gcaccggctc
     8461 gccgaccacg aggccgacct cctgtgccgg caactcggcc tcggtgcctt cctccatgcc
     8521 gaagggcgcg acgcacagcg cctcgaccgg cggctcgatg ccgggcacgg ccggcatcga
     8581 gctttcgatg ccgacgtaat agctgcgcgc cgtgccgccg cggatgcgca cgccgcggcc
     8641 gcggcgcacg cagccgtaat aggccgcgcc gcgtgcgacc gcgtgatcga gctgcgtgcc
     8701 gggcaggcgc cgggccggcg gcgcgccctc ggcggcgagc caggcgttca gcacgtcgag
     8761 cacgcgcgct tcgagcgcgg ccgccttgaa cacgccgccg ttgaacagca ccgcagtcgg
     8821 gtgcaggaag ctcgcgccgg ccggcagctc gatgccgcag ccgggtgccg cgtcgagctg
     8881 gcgggcgagg aaggcggcga ggtggcgggt gacgccggcg tcctgcgcat acggcaggcc
     8941 gagccgggtc aggccggtgc gcgcgcgcgc cagcggccgg gcctcggcgc cgacgcgcgg
     9001 gaagaagccc tcgaccagcg tcgcctcgac ctcgacgcgc gtcagctcgg tgcgcagcgt
     9061 gccgccgatc agccgcgagc cgcggctcgg caccacgacc ggcaccgcat cggccgcggc
     9121 atcgccgagg atctgctcct tggcggcgcg acagccgtgc acgaggccct gcacctgcca
     9181 gggttcgagc tgcgtgccgg cggcggcgag cttggcgcgc agcacgtgtg cgagcgccag
     9241 gtccatgttg tcgccgccga gcaggatgtg atcgccgacc gcgatgcgcg tgaagtcgag
     9301 cccgccctcg cgctcgccga ccgcgatcag cgagaagtcc gccgtgccgc cgccgacatc
     9361 ggcgacgagg atcacgtcgc cgacgcgcgc ctgcttgcgc cagccgccgg cggtcgcctc
     9421 gatccacgaa tacagcgccg cctgcggttc ttcgagcagg atgaagtctt cgaggccggc
     9481 ggcctgcgcg gcctcggcgc tcagctcgcg cgcggccggg tcgaacgagg ccggcaccgt
     9541 gatcgtcagc gcctgcgcat cgagcggcgc gtcggggtgg cgcgcgtccc aggcatcggc
     9601 gaggtgttcg aggtagcgca ccgtggcatc gaagggcgag atgcgcgcga cctcggccgg
     9661 cgcctcgacc ggcagcaccg ggccgcggcg gtcgatgccg gggtggcaga gccagctctt
     9721 ggcgctcgcg accaggcgca gcggcgtctt ggcgccgagg ctgcgcgcga gctcgccgac
     9781 caggaagccc tcgggcgtgt cccagggcag cgccagatcg gccggtgcca gctcatccgg
     9841 atgcggcagg tacaggaagg acggcagcag cgtgcgctcc tcgacggcgc cgggcgcggt
     9901 gagctgcgca accggttcga gggcgagcgc gacggtctcg ccgtcggtca ggctcaggtc
     9961 ggcggcggcg agcacgcagt gcgtggtgcc gagatcgatg ccgacggaat aacggggttc
    10021 gctcatggcg gtctccggag agggacggaa gcggtggccg cggggggcgc cgggaggcgc
    10081 tccggtgaag gcttcggcgc cgggcctaca gctcgacctc ggccggtgcg acgacgtgca
    10141 cgtcgtggcc ctcggcgagc ttcggcagct tcacggcact cgcgcgccag ccgcagtgca
    10201 ccagcgtgcc ggtgaacggc ggctggccga gcacgcggcc ggtcaggcgc acggcaccgg
    10261 catcgaagcc ggccggcagc gtcagccggc tgccctcgcg ctcgctgcgc accggcgcca
    10321 gctcgaagtg cgtgcgcagc accttgcggc agccctcgtg gacgatgcgc gccgcggcgc
    10381 cgatgtcggc atcgccggcc gcaccgaggt cctcctgcag gaagtcgatc agccggccct
    10441 cctgctgcag caggccgagc agctgcagcg cggcatcggg cgtgacctcg cgcagcacca
    10501 ccggcggcgc caccggggcc ggaggcggcg ggggcacggc agcgagcggc tccggcatca
    10561 ccaccggcag cgcggcggct gcgcgctcac cggcatcgcc gtggcgcagc cgggccacgc
    10621 cggcggcaaa gcggcccgag gccagcgtgc ggccgaaggc actgaaggcc aggaagacac
    10681 gttcgaagat cgagggcgtc ggttcgctca cgggcgggcg ctcctgcagg tccggttcga
    10741 ttccggttca gaggggggac agggacggaa gccgcgcgca gcggcctgcg cgcggtgcgg
    10801 aaggcggtgc atggcggccg gaatcagcgc agcggactgt cgagcccgaa ggcgcgggtc
    10861 agtgcgcctg ccagcgtcga ttcgacctgg ccgaacagcc gctcgaaggg tttttcctgc
    10921 tgcgtgtggt cgcggatctg cgtggtgccg agcttgtcgc gggcgatgga ttcgacgctg
    10981 ccgtagccat cgaccagccc gagcgcctgc gcctgctcgc cggtccagaa caggccgctg
    11041 aagatctccg gcgtctcgtg caggcgctcg ccgcggcctt cgcgcaccgc ggcgatgaac
    11101 tgctggtgga tggcatcgag cagcgtctgc acgtgcgcga gctgcgccgg ctgcaccggg
    11161 ctgaacggat cgagcaggcc cttgttggcg cccgaggtca gcagccgccg ttcgatgccg
    11221 aggcgctgca tggcctcgac gaaaccgaag ccgtccatgc gcacgccgat cgagccgacc
    11281 aggctggcgc gatcggcgta gatctcctgc gcgccggcgg cgatgtagta cgcaccggag
    11341 gcgccgacat cgccgatcac cgcgtacacg ggcttgtccg gatgctgcgc gcgcagccgg
    11401 cgcagttcgg cgtagacctg tccggcctgc accgggctgc cgccggggct gttgatgtcg
    11461 aggatgatgc cggccgcctc gcgatcggcg aaggccgtgc gcagaccgtg cagcacgcgc
    11521 tcggcgctgg cgtcggtgcc cggggcgatc aggccgtcga cgctgacctt cgccgcgtgc
    11581 cggccgccgc cgagcgtggc cgccgtcatg tcgctgccgg cgcgccactg cgcgagcacg
    11641 agcagcacca cgatcagcca ggcggcacgg aagaacagcc gccagcgccg ctcgctcgtc
    11701 cccagccgca cgacttccag catcct
//
LOCUS       DAOQIE010000089        11687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2717_length_11687_cov_6.342417, whole genome shotgun sequence.
ACCESSION   DAOQIE010000089 DAOQIE010000000
VERSION     DAOQIE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_11687_cov_6.342417"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..2211)
                     /locus_tag="PK144_07300"
     CDS             complement(259..2211)
                     /locus_tag="PK144_07300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNF65571.1"
                     /translation="MKFRPDIEGLRAVAVMLVVAAHARIDFLAGGFIGVDVFFVLSGY
                     LITGLLVQELTTHGRIDLPRFYARRFRRLFPSLLVVIAISSLLGQQLLAIGEQPGQAL
                     AAASAIVWLSNFYFSFSELDYFSAGAEGNLFLHTWSLGVEEQFYLIWPALLILLATAG
                     RPDSGQANPGRLRRALPVITVLGLLVSIIWTALDPLPAYYLLPTRVWQFALGALVYLH
                     FGGQTPRAVAPGLLRPAGWLGLAAILAAALLFDGQSGYPGFLAALPSVGAAVVLASGA
                     HLSRSGVGWLLSRAPMQAIGRVSYVWYLWHWPVLLLGAAVLDASRLEVRAGLALLALL
                     LAALTHVLIEAPLRDARRHPLPVRAVLAGTMLTMFITSGLALNWHEDSLQEAGNAQQQ
                     PYEQARLDAPVIYAMGCDDWFHADTVKACEFGTGKAGKTAVVLGDSIGLQWFPAFESV
                     FVPRDWRLLVLTKSSCPMVDEPIFYARIGRRYEECERWRANALQAIAQLHPDMVILGS
                     TYTYDFSESQWREGTARLLTPLAAAAGRVYLLRSTPVLPFDAPSCLAPRGWLYQQIPD
                     QDRCAAALRDPRHDDVDRWIRSAAAAFSNVKVVDMSDAVCPGGVCRAQQHGMVAFRDT
                     QHLSAGFARSLSDDLARRLNPDAAGL"
     assembly_gap    2758..2857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2858..2967)
                     /locus_tag="PK144_07305"
     CDS             complement(<2858..2967)
                     /locus_tag="PK144_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF65572.1"
                     /translation="MSVPAIRLSLDAFLDWEATQPERHEYVRGEVFAMTGA"
     gene            complement(2984..4702)
                     /locus_tag="PK144_07310"
     CDS             complement(2984..4702)
                     /locus_tag="PK144_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNF65573.1"
                     /translation="MSVRCRSLLHWFAVLVMCTAGTTVPADPIEVNPTYKVHMLSKPA
                     VVRILDGAIGFIPLADGRKVELQSISSGSGFFVSPDGYIMTNAHVVSMTKSGDEGIRQ
                     ELFKQFILKALASRNMQPTQENVAAVLRWAQQNAGQVTLTTRANFVLLQDGNKLRYEI
                     KSYGAPMGEEGGQLTGKDVAILKVETRNAPTLRLGDSARMRVGDRVFVIGFPGAADSD
                     LLDNRSSVEPTTNDGSISAVKTTVDGIPVLQTNTNTTHGNSGGPVLNQNAEVIGLLTF
                     RGNTVNGQEVQGFNFIVPSNTAREYLGPAGTTNTAGLVDQGWSAGLEQYWAGHFRTAK
                     TKFEEVVSLQPNHAEARRLITETQERILRGEEKPDAPAPAPLPIALTPPAPGPATAPV
                     PTPAPAADGTGTTLIIAIAAIAVLIVIAVAVLLLRNRGGGRPAPTTRAPYAGPSLRGG
                     TAHLPAYAGDSAATTFGAPTGEARLSFTAGPLSGQNISIPASGAWLGRDASRADILVS
                     HPMVSGQHLWIGRQGERWLLKDKGSTNGTYIGSSGSGRVSEHPLRNGDRVLIAPDGAV
                     SFTVSL"
     gene            complement(4878..6101)
                     /locus_tag="PK144_07315"
     CDS             complement(4878..6101)
                     /locus_tag="PK144_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNF65574.1"
                     /translation="MLRRQLLKAIAAAPLLALGPRLYGAELPQPGPRLVVVLLRGGLD
                     GLSAVVPWADADYRAARPRIGLGAPGTAGGVLDLDGRFGLHPALAPLMPLWSAGLLAC
                     VHACGSPDPTRSHFDAQDDLDTGTPGRRSTASGWLNRLLTELPQARGPTAGLSVGAQI
                     PRLLSGPRPVAMLASGRGAGRASAMDRPGLATALDRLYAPDDALGRAWRTGRAARAEL
                     MAGLNGADAGADREAEDTARGWSGDAIRLARLMRNEPKLRVAALTLGGWDTHISQGSA
                     EGQLARRLGPLADGLAAFAQALGPLLAETLVVVVSEFGRTVRENGNSGTDHGHGGVLW
                     LLGARVHGGRMHGDWPGLGEAARWQGRDLAVSTDFRTVLWRALGAHFALDDAALARVL
                     PGFKFPAGALDGLTG"
     gene            complement(6138..7826)
                     /locus_tag="PK144_07320"
     CDS             complement(6138..7826)
                     /locus_tag="PK144_07320"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNF65575.1"
                     /translation="MSRSLLCSVLLTGWLLLPPAAAAAPAPAPLSDAERTLHVLDRLA
                     YGPRPGELDAVAAEGTDAWIARQLQPDSLPLPPALLAELEALPTHGLDAAEIWRRYGP
                     PAQAALPVKERPLAEAKAKRLVLAAEQRERLLRALASPRQLEEVMTEFWFDHFNVHRE
                     KDAVMLWVGDYERSAIRPHALGRFRELLGATARHPAMLYYLDNWRSGAGGQNENYARE
                     LLELHTLGADGGYTQADVIALARILSGWTLDLAGAAEPGAPLFRFQPGRHAGGDKRLL
                     GQRIPAGGEDEGEAALDLLARHPSTARHIAYELAQRFVADQPPKPLVALLAKRFRDTD
                     GDLRAVLATLFESPQFTARAAWRAKFKTPYRYVLSAWRAGQLPIDDTAPVMTALDRLG
                     QPLWGCPTPDGYGDTAADWLGPDALRLRVDLATAWASGRLPGRDAGPDKSGRRRDAGR
                     MSPPAAGEASMAPMAPMNPSAMAAAGADREAGSEPPEAAPAMAMTPPMRSPAEAAVRR
                     SGPVPADPALAPDAASLAATLGPGIGRRTAAAVASASPPLRSVLLLGGPDFMRR"
     gene            complement(7962..8567)
                     /locus_tag="PK144_07325"
     CDS             complement(7962..8567)
                     /locus_tag="PK144_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNF65576.1"
                     /translation="MTAPAEIKVWDPLVRVFHWSLVVAFTLAFLTEDDWQWLHVIAGY
                     TVLGLVLVRLVWGLVGPRHARFSDFVHRPRTVWQYTQAVLRGRAERHIGHNPAGGAMI
                     VALLVMLLVTTGLGLCAYGARGNGPLGDWLGELSAFATDALTEAHELAAWLTVWLIAG
                     HLLGVLWESLLHRENLVRAMITGRKRAAKLPDGLPADPQAP"
     gene            complement(8583..>8818)
                     /locus_tag="PK144_07330"
     CDS             complement(8583..>8818)
                     /locus_tag="PK144_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNF65577.1"
                     /translation="LLAAALLATAGSAFAATLTKEEAIAKAQAIHPGEVIKAYTEVQH
                     GRTLWEVQIKGEDGKKAAIFIDAETGEQDLKAK"
     assembly_gap    8819..8918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8919..10945)
                     /locus_tag="PK144_07335"
     CDS             complement(<8919..10945)
                     /locus_tag="PK144_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65578.1"
                     /translation="MLLNRLKVWQKLLLLAVLGAVLVAVPLGLLVRDELAARTLNRHQ
                     ADGIEPLRQTLQAVQLVQQHRGLSAQTLSGRTDAGATRNGKAQAITAALAALDAIVRA
                     RSDDVVLTSFWQQLPGTWQALATSVGNAQVEPAAAFAAHTALIQRLLDLLGLIANHHD
                     LALTPDKADYFLQRAIIDQLPPLAELMGQTRARGVAVLTVRNDGTVTADSLTSARATG
                     GTTRSSISEADRLEMQRLHDAIQHQARSVSGTLTAITELDPALGERLRTPVTAANAQV
                     EELAQLVLWEFIEADTIRFDAGEFFRISTAAIDVQFALIDSLLDALETRIEVQAGALQ
                     RQQQLLAAAMVGIALLTGGLTLAIVRTITAPLSQTARVLQTAAGGDLTRRLRPRSRDE
                     LGHMGRDLDVFLDALSRSIGEIADTAQQLGQSSQALTEVSRRVNDGAARTTTDAEKLS
                     IAAADMNERVASVATATSQMNTAVHEIARSTAEGARVTGEAVSLASAATTAMQRLDES
                     SARIGEVVALISHITRQTRLLALNATIEAARAGEAGRGFGVVAHEVKELADGTARATQ
                     DIADKIGTIQSDTAGAVEAIRGIGDSVGRVNTLQTTIAAAIEEQSVVTDDISRNIAQA
                     ATRTGEIGSGLIEVTQIAAGAAASGRELESAAHALAQMGAQLRDLVQRFRYA"
     gene            11283..11471
                     /locus_tag="PK144_07340"
     CDS             11283..11471
                     /locus_tag="PK144_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65579.1"
                     /translation="MVSVEAKLVLFEVKPVLSAAELVMSEVKPVLSGGELVMSGVKPG
                     FVLSGTGYVRSETGFRFK"
BASE COUNT         1648 a         3841 c         4293 g         1705 t
ORIGIN      
        1 tacgtcattc tcgatctcga tcatccgcgc gccggcctga ttcaactgga ttatgtcgat
       61 caggcgctgg ccgatgtcct cgccggcatg aaatgacggc ggcgctggca ttgttcgccg
      121 gccgtcccgc aatggatgcc gccttcccgg cccggggcct ctgcgatccg cgatcgctca
      181 tgcggtcacc ggccgtgatc ccggtgcccg ttgtcgggaa tcacggacgc agcaggcgtg
      241 tggcagcgaa ctgccggatc acaggccggc ggcatcggga ttcagccgcc gggccagatc
      301 gtcgctcagg gagcgcgcaa acccggcgct caggtgctgc gtgtcgcgga aggcgaccat
      361 gccgtgctgc tgcgcgcggc agacgccgcc ggggcagacg gcgtcgctca tgtcgacgac
      421 cttcacattg ctgaaggccg cggcggcgga ccggatccag cggtccacgt cgtcatggcg
      481 ggggtcccgg agcgcagcgg cgcatcggtc ctgatccggg atctgctggt acagccatcc
      541 gcgcggggcc aggcaggacg gcgcatcgaa gggcaggacc ggcgtcgagc gcagcaggta
      601 gacccggccg gccgcggcgg cgagcggggt cagcagccgt gccgtgcctt cccgccactg
      661 gctttcgctg aaatcgtagg tgtaggtcga gccgaggatc accatgtccg ggtgcagttg
      721 cgcgatggcc tgcagggcgt tcgcgcgcca gcgttcgcat tcctcgtagc ggcgcccgat
      781 ccgggcatag aagatcggct cgtccaccat cgggcacgac gatttggtca gcaccagcag
      841 acgccagtcc cggggaacga acacggattc aaacgctgga aaccattgca gaccgatgct
      901 gtcgccgagc acgaccgcgg tctttcccgc cttgccggtg ccgaattcgc aggctttcac
      961 cgtatcggcg tgaaaccagt cgtcgcagcc cattgcatag atcaccggcg catcgaggcg
     1021 ggcctgttca tagggttgtt gctgcgcgtt tccggcttcc tgcaggctgt cctcgtgcca
     1081 gttcagcgcc agtccgctgg tgatgaacat cgtcagcatc gtgccggcca gcacggcgcg
     1141 cacgggcagc gggtggcgcc gggcatcgcg cagtggcgct tcgatcagca cgtgcgtcag
     1201 ggcggcgagc agcagtgcca gcagcgcaag ccctgcgcgc acctccagcc gcgacgcatc
     1261 cagcacggcg gcaccgagca gcagcaccgg ccagtgccac agataccaga cgtaggaaac
     1321 ccgcccgatc gcctgcatcg gcgcccgcga cagcagccag ccgacgccgg atcgcgacag
     1381 gtgcgcaccg cttgccagca ccaccgccgc gcccacgctc ggcagggcgg caagaaagcc
     1441 ggggtagccc gactggccat cgaacagcag cgcggccgcg aggatggcgg caagtcccag
     1501 ccagcccgcc gggcgcagca ggccgggggc gacggcccgg ggcgtctggc cgccgaagtg
     1561 cagatacacc agcgcgccca gcgcgaactg ccagacgcgg gtcgggagca ggtaataggc
     1621 cggcagcgga tcgagtgccg tccagatgat gctgacgagc aggccgagga cggtgatcac
     1681 cggcagtgcc cgccgcagcc gtcccggatt cgcctggccg ctgtcggggc gtccggccgt
     1741 ggccagcagg atcagcagcg cgggccagat caggtagaac tgctcctcga cgcccagcga
     1801 ccaggtgtgc aggaacagat tgccctcggc tcccgcactg aagtaatcga gttcggaaaa
     1861 cgaaaaataa aagttgctga gccagacgat cgcgctggct gcggccagcg cctgccccgg
     1921 ctgttcgccg atggccagca attgctggcc gagcaggctg ctgatggcaa tgacgaccag
     1981 cagcgaagga aacaggcgcc ggaaacggcg ggcatagaac cgtggcagat cgatgcgtcc
     2041 gtgcgtcgtc agttcctgca cgagcagacc ggtgatcagg taaccggaca gcacgaaaaa
     2101 cacgtcgacg ccgatgaagc ctccggccag aaaatcgatc ctggcgtgtg ccgccacgac
     2161 cagcatcacc gcgaccgcac gcaatccttc gatgtcgggg cgaaatttca cgggtggtgt
     2221 ccttgttgtc gattggaaaa acaggcaggc gccatcgcgc agggcgatgc cgtctggcgg
     2281 caatcccgcc gcttgcctcc ggggcaggcg ttcgttcagg gaagccgggc gcacgctgcc
     2341 cggccccggg attttacgct gcagccgcag cgcgccgtaa ccggtctttc cgaagggcgc
     2401 atgcggatgc ccgatggcgg ataccggtca caccgcaagc cttcgctttt cagttcctcg
     2461 tgcctcgtgg gagaggtgtc ggggagaaaa gaggaaaacc atgcacttgc gtgtgatcgg
     2521 catgacgcat cgggcaaggc gggttctgcc tgaaacgccg gcagcgtgca ggcgcgaggg
     2581 tgtgccgcgg cttcaggccc gggatccgcc atccgccggc ggctcgaagc gcacgtcctc
     2641 gtacaccgcc gcgatcggca cgcacagatc gatgctggcg aatccgaccg tgtcgccggc
     2701 ctcggcggcg tgcagcgtcc agccgtcggg gctgcggcgg aagacctgca ccagcggnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ccggtcatcg caaacacttc
     2881 gccgcgcacg tactcgtggc gttcgggctg cgtcgcctcc cagtcgagga aggcgtcgag
     2941 gctcaggcgg atggcgggaa cggacatcgg gcttctcccg ggatcacagg ctgaccgtga
     3001 agctgaccgc accgtccggg gcgatcagca cgcggtcgcc gttgcgcagc ggatgctcgc
     3061 tgacccggcc gctgccggag ctgccgatgt aggtgccgtt ggtcgagccc ttgtccttca
     3121 gcagccagcg ttcgccctga cggccgatcc acaggtgctg gcccgagacc atcgggtgcg
     3181 agaccaggat gtcggcgcgg ctggcgtcgc gcccgagcca ggcgccgctg gccgggatgc
     3241 tgatgttctg gccgctgagc ggaccggcgg tgaagctgag ccgtgcctcg ccggtgggag
     3301 cgccgaacgt ggtcgcggcg ctgtcgccgg cgtaggccgg caggtgggcg gtgccgccgc
     3361 gcagcgacgg gccggcgtag ggggcgcgcg tcgtcggtgc cggccggccg ccgccgcggt
     3421 tgcgcagcag cagcacggcg accgcgatca cgatcagcac ggcgatggcg gcgatcgcga
     3481 tgatcagcgt cgtgccggtg ccgtctgcgg ccggtgcggg cgtcggcacc ggcgctgttg
     3541 ccgggcccgg cgccgggggc gtcagtgcga tcggcagcgg ggccggtgcc ggggcgtcgg
     3601 gcttttcctc gccgcgcagg atgcgttcct gggtctcggt gatcagccgg cgcgcctcgg
     3661 cgtggttcgg ctgcagcgag acgacttcct cgaacttggt cttggcggtg cggaagtgtc
     3721 cggcccagta ctgctccagc ccggcgctcc agccctgatc gaccagaccg gcggtgttgg
     3781 tggtgccggc cgggccgagg tattcgcgtg cggtgttcga cggcacgatg aagttgaagc
     3841 cctgcacttc ctgaccgttg acggtgttgc cgcggaaggt cagcaggccg atgacctcgg
     3901 cgttctggtt cagcaccggg ccgccggagt tgccgtgcgt ggtgttggtg ttggtctgca
     3961 gcaccgggat gccgtcgacc gtggtcttga ccgccgagat cgagccgtcg ttggtggtcg
     4021 gctcgaccga ggagcggttg tcgagcaggt cggaatcggc ggcgccgggg aagccgatca
     4081 cgaacacgcg atcgccgacg cgcatgcgcg cggaatcgcc gaggcgcagc gtcggggcgt
     4141 tgcgggtctc gaccttgagg atggcgacat ccttgccggt gagctggccg ccttcctcgc
     4201 ccatcggtgc gccgtaggac ttgatctcgt agcgcagctt gttgccgtcc tgcagcagca
     4261 cgaagttggc gcgggtcgtg agcgtcacct gcccggcgtt ctgctgcgcc cagcgcagca
     4321 ccgcggcgac gttttcctgc gtcggctgca tattgcggct ggccagcgcc ttcaggatga
     4381 actgcttgaa cagctcctgg cggatgccct cgtcgcccga tttcgtcatc gagaccacgt
     4441 gggcattggt catgatgtag ccgtcgggcg agacgaagaa gcccgagccg ctgctgatcg
     4501 actgcagttc gaccttgcgc ccgtcggcga gcggaatgaa gccgatcgcg ccatcgagga
     4561 tgcgcacgac cgccggcttc gacagcatgt gcaccttgta ggtcgggttc acctcgatcg
     4621 gatcggccgg cacggtcgtg ccggccgtgc acatcacgag cactgcgaac caatgcagca
     4681 gcgaccggca gcggactgac atctcgttct tctcccccgg gaacatggat cggtctggcg
     4741 cgcgggtggc acggtacggt gcggtacgga tcggcggcgc gccggtacgg acggaaacga
     4801 tggccgagcc gcgcgccccg cacaagggag ccggtcagga tcggggcggg gccgggcgat
     4861 gcgcgtgcgg gttcgcgtca gcccgtcagc ccgtccagag ccccggcagg gaacttgaag
     4921 ccgggcagca cacgcgcgag cgcggcatcg tcgagcgcga agtgtgcgcc gagcgcacgc
     4981 cagagcacgg tgcggaaatc cgtgctgacg gccagatccc ggccctgcca gcgggcggct
     5041 tcgccgagcc ccggccagtc gccgtgcatg cggccgccgt gcacccgggc gccgagcagc
     5101 cagagcacgc cgccgtgacc gtggtcggtg ccgctgttgc cgttctcgcg cacggtgcgg
     5161 ccgaactccg agaccacgac gaccagggtt tcggcgagca gcggtccgag ggcctgcgcg
     5221 aaggcggcga ggccgtcggc gagcgggccg agccggcggg cgagctggcc ttcggcgctg
     5281 ccctggctga tgtgggtgtc ccagccgccg agcgtgagcg cggcgacgcg cagcttcggt
     5341 tcgttgcgca tcagccgcgc cagccggatc gcgtcgccgc tccagccgcg cgcggtgtct
     5401 tcggcctcgc gatcggcgcc ggcatcggcg ccgttgaggc cggccatcag ctcggcacgc
     5461 gcggcgcggc cggtgcgcca ggcgcgcccg agcgcgtcat cgggtgcgta cagccggtcg
     5521 agcgccgtgg cgaggccggg gcggtccatg gcgctggcgc ggccggcgcc gcggccgctc
     5581 gcgagcatcg ccaccggccg cggtcccgac agcagccgcg ggatctgcgc accgaccgac
     5641 agcccggcgg tcgggccgcg ggcctgcggc agctcggtga gcaggcggtt cagccagccg
     5701 gaggcggtcg agcgccggcc gggcgtgccg gtgtcgagat cgtcctgcgc atcgaagtgc
     5761 gagcgcgtcg ggtccggcga gccgcaggcg tgcacgcagg cgagcaggcc ggcgctccag
     5821 agcggcatca gcggcgcgag cgccggatgc aggccgaagc ggccgtcgag gtcgagcacg
     5881 ccgccggccg tgcccggtgc gccgagcccg atgcgcggtc gcgcggcgcg gtaatcggca
     5941 tcggcccagg gcacgacggc actgaggccg tcgagcccgc cacgcagcag cacgaccacc
     6001 agccgcggac ccggctgcgg cagttcggct ccgtacagcc gcggaccgag cgcgagcagc
     6061 ggggcggcgg cgatggcttt cagcagttgg cgacgcagca tgggtcttcg acctccggcg
     6121 gcggcggggg atgacaacta gcgacgcatg aagtccggac cgccgagcag cagcacgctg
     6181 cgaagcggcg gcgaggccga ggcgacggcc gcggccgtgc gccggccgat gccggggccg
     6241 agcgtggcgg cgaggcttgc ggcatcgggc gcgagcgcag gatcggctgg caccggacct
     6301 gaacggcgga cggccgcttc ggccggcgag cgcatcggcg gcgtcatcgc catggccggc
     6361 gctgcttcgg gcggttcgga gccggcttcg cggtctgcgc cggccgcggc cattgccgac
     6421 ggattcatcg gggccatggg ggccatcgat gcctcgccgg cggctggcgg cgacatcctg
     6481 ccggcgtcgc gccggcgccc ggacttgtcg gggccggcat cgcgccccgg cagccggccg
     6541 ctcgcccagg ccgtggccag atcgacgcgc aggcgcagcg catcggggcc gagccagtcg
     6601 gcggcggtgt cgccgtagcc gtccggcgtc gggcagcccc acagcggctg gccgaggcga
     6661 tcgagcgcgg tcatgaccgg cgcggtgtcg tcgatcggca gctgcccggc gcgccaggcc
     6721 gacagcacgt agcggtacgg cgtcttgaac ttcgcgcgcc aggccgcgcg cgccgtgaac
     6781 tgcgggcttt cgaacagcgt tgccagcacg gcgcggaggt cgccgtcggt gtcgcggaag
     6841 cgcttggcga gcagcgcgac cagcggcttc ggcggctgat cggcgacgaa gcgctgcgca
     6901 agctcatagg caatgtgccg cgccgtcgac ggatggcgcg cgagcaggtc gagggcggcc
     6961 tcgccttcgt cctcgccgcc ggccgggatg cgctggccga gcagccgctt gtcgccgccc
     7021 gcatgacggc cgggctggaa gcggaacagc ggcgcgccgg gctcggcggc cccggcgaga
     7081 tcgagcgtcc agccgctcag gatgcgcgcg agcgcgatga catcggcctg cgtgtagccg
     7141 ccgtcggcgc cgagcgtgtg cagctccagc agttcgcgcg cgtagttctc gttctggccg
     7201 ccggcgccgc tgcgccagtt gtcgaggtaa tacagcatcg ccggatggcg ggcggtggcg
     7261 ccgagcagct cgcggaagcg cccgagcgcg tgcgggcgga tggcgctgcg ctcgtagtcg
     7321 ccgacccaca gcatcacggc atctttctcg cgatggacgt tgaagtgatc gaaccagaac
     7381 tcggtcatca cctcttcgag ctggcgggga cttgcgagcg cccgcagcag gcgctcgcgc
     7441 tgctcggcgg cgagcacgag gcgcttggcc ttcgcctcgg cgagcgggcg ctccttcacc
     7501 ggcagcgccg cctgcgccgg cggaccgtag cggcgccaga tttcggcggc atcgaggccg
     7561 tgcgtcggca gcgcttcgag ttcggcgagc agcgccggcg gcagcggcag cgaatcgggc
     7621 tggagctggc gggcgatcca ggcgtcggtg ccttcggcag cgacggcatc gagctcgccg
     7681 ggccgcggcc cgtaggcgag ccggtcgagc acgtgcagcg tgcgttcggc gtcggaaagc
     7741 ggagcgggag cgggggcggc agcggcggcc gggggcagca gcagccagcc ggtgagcagc
     7801 acggagcaca gcagggatcg tgacatctgc gccacctgac gcacgccggt gacccggcgt
     7861 gcctgaggaa tcaggggctt gagcttactg gagcgtggcg gtcgcgggta tccggcatcg
     7921 gcccggggcg acaggatttg cgtttcgtga ccgatcggcg ctcatggcgc ctgcgggtcc
     7981 gccggcagcc cgtccggaag ctttgcggcg cgcttgcggc cggtgatcat cgcgcgcacc
     8041 aggttctcgc ggtgcagcag actctcccag agcacgccga gcagatggcc ggcgatcagc
     8101 cacaccgtca gccaggcggc cagctcgtgc gcttcggtca gcgcatcggt cgcgaaggcg
     8161 ctcagctcgc cgagccagtc gccgagcggt ccgttgccgc gcgcaccgta cgcgcagagg
     8221 ccgagtccgg tcgtcacgag cagcatcacg agcagcgcga cgatcatcgc gccgccggcc
     8281 ggattgtggc ctatgtgacg ctcggcgcga ccgcgcagca ccgcctgcgt gtactgccag
     8341 acggtgcgcg gccggtgcac gaagtcgctg aagcgcgcat gccgcggacc gaccagcccc
     8401 cagaccaggc gcacgagcac cagcccgagc accgtgtagc cggcgatgac gtgcagccac
     8461 tgccagtcgt cctcggtgag gaaggccagc gtgaacgcca cgaccagcga ccagtggaag
     8521 acccgcacca gcgggtccca gaccttgatt tcagcggggg cggtcatgtc ggcagcccgc
     8581 gcttacttgg ccttcaggtc ctgctcgccg gtctcggcgt cgatgaagat cgccgccttc
     8641 ttgccgtctt cgcccttgat ctgcacttcc cacagcgtgc ggccgtgctg gacttcggta
     8701 taggccttga tgacttcgcc ggggtggatg gcctgggcct tggcgatcgc ctcctccttg
     8761 gtgagggtgg cggcgaaggc gctgccggcg gtggcgagca gggcggcggc gagcagggnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gtagcggaag cgctgcacga
     8941 gatcgcgcag ctgcgcgccc atctgcgcca gtgcgtgcgc ggcgctttcc agctcccggc
     9001 cggaggccgc ggcgccggcg gcgatctgcg tgacctcgat gaggccgctg ccgatctcgc
     9061 cggtgcgcgt ggccgcctgc gcgatgttgc ggctgatgtc gtcggtgacc accgactgtt
     9121 cctcgatcgc cgcggcgatc gtggtctgca gcgtgttcac gcgtccgacg ctgtcgccga
     9181 tgccgcggat cgcctcgacg gcgccggcgg tgtcggactg gatcgtgccg atcttgtcgg
     9241 cgatgtcctg cgtggcgcgg gccgtgccgt cggcgagttc cttgacctcg tgcgcgacca
     9301 cgccgaagcc gcgtccggcc tcgccggcgc gcgccgcctc gatggtcgcg ttcagcgcca
     9361 gcagccgcgt ctggcgcgtg atgtggctga tcagcgccac gacctcgccg atgcgcgcgc
     9421 tgctctcatc gaggcgctgc atcgcggtcg tcgccgcgct ggcgagcgac accgcctcgc
     9481 cggtgacgcg cgcgccctcg gccgtgctgc gtgcgatctc gtgcacggcg gtgttcatct
     9541 ggctggtggc ggtcgcgacg ctggcgacgc gctcgttcat gtcggcggcc gcgatgctga
     9601 gcttctcggc atcggtggtc gtgcgcgccg ccccgtcgtt gacgcggcgg ctgacctcgg
     9661 tcagcgcctg cgacgactgc ccgagctgct gcgcggtgtc ggcgatctcg ccgatgctgc
     9721 gcgacagcgc atcgaggaac acatcgaggt cacgccccat gtgtccgagc tcgtcgcgcg
     9781 agcgcgggcg cagccgccgg gtcagatcgc cgccggcggc ggtctgcagc acgcgcgccg
     9841 tctgcgacag cggtgcggtg atcgtgcgca cgatcgccag cgtcagcccg ccggtcagca
     9901 gcgcgatccc gaccatggct gcggcgagca gctgctgctg gcgctgcaac gcgcccgcct
     9961 gcacttcgat gcgggtttcg agcgcatcga ggagcgagtc gatcagggcg aactggacat
    10021 cgatcgccgc cgtgctgatg cggaagaact cgccggcatc gaagcggatg gtgtcggctt
    10081 cgatgaattc ccagagcacc agctgcgcca gttcctcgac ctgcgcattc gccgccgtga
    10141 ccggcgtgcg cagccgttcg ccgagcgccg ggtccagttc ggtgatggcg gtgagcgtac
    10201 cgctgacgct gcgtgcctga tgctggatcg cgtcgtgcag ccgttgcatc tccagccggt
    10261 cggcctcgct gatgctgctg cgggtcgtac cgccggtggc ccgtgccgac gtgagcgaat
    10321 cggccgtgac cgtaccgtcg ttgcggaccg tgaggactgc cacgccgcgc gcgcgcgtct
    10381 ggcccatcag ctcggcgagc ggcggcagct ggtcgatgat cgcgcgctgc aggaagtaat
    10441 cggccttgtc cggagtcagc gccagatcgt ggtgattggc gatcagcccc agcagatcga
    10501 gcagccgctg gatcagggcg gtatgggcgg caaaggctgc ggccggctcg acctgggcgt
    10561 tgccgaccga ggtggccagc gcctgccagg tgcccgggag ctgctgccag aacgaagtga
    10621 gcacgacatc gtcgctgcgc gcccgcacga tggcatcgag cgcggccagc gccgcggtga
    10681 tggcctgcgc cttgccgttg cgggtggcgc cggcatcggt gcgtccggac agcgtctgcg
    10741 ccgaaagtcc gcggtgctgc tgcacgagct gcacggcctg cagcgtctgg cgcaagggct
    10801 cgatgccgtc tgcctgatgc cggttgagcg tgcgcgccgc gagttcgtcg cgcaccagca
    10861 gcccgagcgg cacggccacc agcacggcgc cgagcacggc gagcaacaac agcttctgcc
    10921 agaccttcag gcggttgagg agcatttgcg tacctgctga gaaacaaagc cgagggggca
    10981 gcgacgcacc tgcgtgcgcg gacatgaccg ggccggtgcg cttgccggcg gccctgcagg
    11041 ccggccgatc agcagatatc ggccgccggg cagggcgctt gagcggcagg cacccggccc
    11101 ggtgcgcgcg cggcggcgcg ggcgggcacg ggcggcggcc ggtgtctggc tgcctgccgg
    11161 ctgcatccgc aggccggcag gttgccggtg cgcgggaaga gctggccggg ggcgggcagc
    11221 ggcttgccgg tggggggaac cggccaggta tttccggaag tcagcctggg gtgattgcca
    11281 acatggtttc cgttgaagcg aaactggttc tgttcgaagt gaaaccagtt ctgtccgcag
    11341 cggaactggt tatgtctgaa gtgaaaccag ttctgtccgg aggggaactg gttatgtccg
    11401 gagtgaaacc gggtttcgtt ctgagtggaa ctggttatgt ccggagtgaa accgggtttc
    11461 gtttcaagtg aaaccggtta tgtccgcagc ggaactggtt ctgtccggaa cgaaaccggt
    11521 tatgtccgga ggagaactgg ttgtgtccgc aacgaatccg ggtttcggtt cgagtgcaac
    11581 cggttcggtc cgcaggggaa ctgcttcggt acgcagcgca acccggtccg tgccgggggg
    11641 agcggattgc gggcgcgggg cagccggctg cgctgcggtg gcagcgg
//
LOCUS       DAOQIE010000090        11674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2722_length_11674_cov_7.003959, whole genome shotgun sequence.
ACCESSION   DAOQIE010000090 DAOQIE010000000
VERSION     DAOQIE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_11674_cov_7.003959"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PK144_07345"
     CDS             <1..541
                     /locus_tag="PK144_07345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF65580.1"
                     /translation="SGGMANPFYVELGYSGSEIAAIAKVFGIWATIVGGLVGGIVVAR
                     YGTVPTLAVGGVLQASTNLLFAALALRGHDLGWLTLAIAADNLAGGLASAAFVAYLSS
                     LCAAAYTATQYALLTSLMAVGMRVFAASGGWLAEQLGWVWFWGGSALLALPGLLLLWW
                     LQRQGVARAPAAAQEAGAG"
     gene            complement(658..1890)
                     /locus_tag="PK144_07350"
     CDS             complement(658..1890)
                     /locus_tag="PK144_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNF65581.1"
                     /translation="MSFLIGLIVALFSAVGLAWLVRQDAGYVLITVGNWTIETSVAAA
                     LVIGVLGFFILYKLIRYLGRLLRMPGTLRAISTRRRERKAARLLALGQKALEEGRLQA
                     AESAFQKGIVLAETNRALYFVGAARAAHRLGAKDRRERYFEQAAKLPRDAVAIVGIAR
                     AEMLLEDDDVEGARKVLHDVQTLAPNQPRVLELQLEIASRIGDWDHALRLLPELRKRK
                     LLDESGFHDGQIQVHRERLASTTRRGVAADVQRAWSGVPRALRNEETLLIEYAGSLRE
                     HDPAEAEKVLKAALAQKWSNPLCIAYGQLGRGDPNAQLATAEGWLSVHKDNPWLLLTL
                     GRLAARAHKLDKARAYLEASLKITPMADTYEALGALLEEHDSPAAVTFYRAGLRLLTG
                     RDEVHTGEALPAQKSLAT"
     gene            complement(1887..3257)
                     /locus_tag="PK144_07355"
     CDS             complement(1887..3257)
                     /locus_tag="PK144_07355"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNF65582.1"
                     /translation="MTDKTTQPGKPSPQAPILTGGKGAPKADDKPAGGKAAPAAPAKA
                     TAVEAVPVRSGSSPLGGIALAVALAAAAGTAYVGYQWWQGRQATIQHDAELDARVKKA
                     ISDSQAQGAKQLADMQAVVKSVQDELAAAKSSLTGQFSGQIADVQKKQAADLTALADR
                     VQTLQLGQRGLFDEVEAAKTAAARGDVNALTLSEVGYLLRIANHKLALEKDPKAALAA
                     LSLAEQRLGAANELAFASVQRMLAENVATVRGVRLPDHAALSARIIELEGKAATLRVR
                     PDIQVDNLKARVKPQLENSVPDSEDPWYERLATNAWNQIKDIVVIKHERTQSQPLMSP
                     KESYFLHENLKLNLEGMRVALLREDAVAFQQSAQLAQGLLKEYFDTADAQVQAMAKEV
                     DGLAQTQFQPYLPDLTGTVQAFNEILARRQPVKSAVGPVTPPAPAAAPAPAATPEAAP
                     AGEVKP"
     gene            complement(3254..4141)
                     /locus_tag="PK144_07360"
     CDS             complement(3254..4141)
                     /locus_tag="PK144_07360"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNF65583.1"
                     /translation="MSTALKGLQVLVTRPAAQSGALAGAIEAAGGFVRSFPALAILPP
                     QDPSAACAALDRLPGCALAIVTSANVVALLRPWLAARGGWPDGVGCLAIGGASARAVR
                     ELGVPVAHDTPGALRSEELLATPELAAGCVRGRRVVILRGEGGRELLGAELGRRGAEV
                     VIAELYRRAPPPDVDPQRLAGWLRDGAIDIVTASSNESLTNLFDLLDARGRESLLSTP
                     LLVVSPRAARLARDLGWSHAPVIATGADAAAIVAALAGWHSARTTEHTTHTITPATTP
                     EPPAITARDPTSDPPEDPT"
     gene            complement(4161..5099)
                     /gene="hemC"
                     /locus_tag="PK144_07365"
     CDS             complement(4161..5099)
                     /gene="hemC"
                     /locus_tag="PK144_07365"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005892713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNF65584.1"
                     /translation="MTDTPTLRIATRKSPLALWQAEHVAARLRAAHPGLAVELVGMST
                     QGDRILDSPLAKVGGKGLFVKELEVALLDGRADIAVHSMKDVPVEFPDGLHLPVILER
                     EDPRDAFVSNAYARFEDLPQGARVGTSSLRRQCQLAARRPDIVIDSLRGNVNSRLAKL
                     DAGQFDAIILAAAGLKRLGFADRIRCELEPETSLPAIGQGAIGIECRRDDARTGALIA
                     VLDDADTHLRLRAERALNTRLKGGCQVPIAGHAILDGDQLWLRGLVGEPDGSRTLRAE
                     RRGPASEAEALGVQLAEDLLALGADVILARVFGAQP"
     gene            complement(5153..6520)
                     /gene="yjjJ"
                     /locus_tag="PK144_07370"
     CDS             complement(5153..6520)
                     /gene="yjjJ"
                     /locus_tag="PK144_07370"
                     /inference="COORDINATES: protein motif:HMM:NF007297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HNF65585.1"
                     /translation="MPAPAPDDLIDALAGLPPQPAQTLCARLGISQSTLSRRLARSAA
                     QVLRIGRARATRYALARSLPSLGSHLPIHRIDADGRVLAVGTLHALSDGGCWCEAVDD
                     TLAAYQWGDNADGVHAGVPYFLWDIRPQGFLGRLLPKQRPELGLPDNLLHWHDDHLLI
                     ALARAGEDLAGELLIGDESLLRWQRQRTQAPLPATADTYAQLAAAVLAGEPAGSSAGG
                     EQPKFAVTVADGDKGGRPVLVKFSPPRSTAGGQRWADLLVCEALALDTLAAQGFAAPA
                     ARIVVGEARTFLEVERFDRCGAHGRRPVLSLAALDAEYVGHGSGWSRVAAGLAATGRL
                     PAAEAARIDWLDAFGALIGNHDRHLGNLALIPGPAGLRLAPVYDMLPMALAPDREGSV
                     REGDGPPPVLPAGPQLIAAAIGYWSRIAADERISAPFRALATRRAGALAAPAGRNGIR
                     LDLEG"
     gene            complement(6643..11463)
                     /locus_tag="PK144_07375"
     CDS             complement(6643..11463)
                     /locus_tag="PK144_07375"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNF65586.1"
                     /translation="MSSSPADKKAELIERVAARLTERLPAGGAVAAQFARVYFARVAP
                     EDLINDDPEDLYGAVLSHWNLLRRTRTGEIGLRVYNPGYDEHGWRSTHTAIELVVPDM
                     PFLVDSVSMELNRHGFTVHRLIHPVLAIAYDEDGEPLSVAETGAGDGVQLAVMQFAID
                     RETDAARLETLRDDLARVLDDVRAAVEDWPRMLARLNEIADELDAAKLPVPVAEVEEA
                     RAFLDWAADNHFTLLGYSGYRLEELDGELVLHPEPGTGLGLRRGAAAHHDTRFNALPP
                     QHRRLALAPVLLVMTKANARSTVHRPVQLDYLGIKRFDEAGRVIGEWRFLGLYTSSAY
                     QTSPFGIPVLRAKVRAVIERAGLPAVSHSGKALKHILATYPRDELLQASVDELYPIAT
                     GILHLEERQHLRVFIRIDAYERYATALIYVPRERYNTDLRKRMQDVLIAALNGHGADF
                     WVEFGESVLARVLFTIRTTPGHIPAYSVEEIRARLRETMLSWEDGLRAALIEAHGEGG
                     GIALYNRYAGAFGAAYQEDFTPRAAVGDIDRLEAVVGGAPLGLHLYRPQESPEGWLRF
                     KLYGRAPVVPLTNVLPMLERMGLAVMETRPYEVEPRGEAGPLWVLDFDLRESAGIHVD
                     VGRVRALFEQAFAQVWAGALENDGFNRLVLGAGLGWREVVVLRAYAKYLLQTRVPFSQ
                     SYMEETLARHAPITGQLAALFVARFDPDHPDAARCEQLAGGIESALENVAVLDEDRIL
                     RRFLAVILATLRTNFYQTSQPGQPGTDGGPKEYLSFKFDPAKIPELPLPRPMFEIWVY
                     SPRAEAIHLRGGRVARGGIRWSDRREDFRTEVLGLVKAQQVKNAVIVPVGSKGGFVVK
                     RPPAEREALMQEVVHCYKTLMRGMLDITDNLVHGEVVPPARVVRYDADDPYLVVAADK
                     GTATFSDIANGLALEYGFWLGDAFASGGSAGYDHKKMGITARGAWESVKRHFRELGKD
                     IQNVDFSVAGIGDMSGDVFGNGMLLSRHIRLIAAFDHRHVFIDPSPDAEASFAERERL
                     FALPRSSWADYDAALISAGGGIWPRTAKSIPLSPEMRAILGTDATRLSPAELINTILR
                     APVELLWNGGIGTYVKAADETHAEVGDRANDAIRVNGSELRCKVVGEGGNLGFTQRGR
                     IEYARAGGHILTDAIDNSAGVNCSDHEVNIKILLNQIVSAGDLTLKQRNELLATMTDE
                     VARLVLRQNVLQPQTISIAVREAPDLLGDHARVIRQLEKAGRLNRRIEFLPPDDEIAE
                     REQAGQGLTAPEIAVLLAYSKIALYDELLDSDVPEDPYLERELRGYFPEPLRERYAEA
                     MERHPLRRQIIATAITNSMLNHMGSTFAFRVNEQCGARSPDIARAYAAARAMFEAPRL
                     WAAIDALDNRAAATLQLDMISDTQALLMRASLWLLRNRRPPIAIEAEVARFAPDITRL
                     LSMKNLMPGRAGATLQAEAARLEGHGVPAETAEWVASLPALYCTLDIIEVANQAQIAA
                     EQVALVYFTLIDALELDWLAATIDRLGAGNHWQHRARSALLDNLYTQQRRLAAAVVAE
                     RGPEEHPQPWIDAWLAHNHAAVERLAAVLTDLKSAPRVDSSMLMVALGEVGAVG"
BASE COUNT         1543 a         4055 c         4340 g         1736 t
ORIGIN      
        1 gtccggcggc atggccaatc cgttctacgt cgaactcggc tacagcggca gcgagatcgc
       61 cgcgatcgcc aaggtgttcg gcatctgggc gacgatcgtc ggcgggctgg tcggcggcat
      121 cgtcgtcgcg cgctacggca cggtgccgac gctggcggtc ggcggcgtgc tgcaggcgag
      181 cacgaacctg ctgttcgcgg cgctggcgct gcgcggccac gacctcggct ggctgacgct
      241 ggcgatcgcc gccgacaacc tcgccggcgg cctggcctcg gcggccttcg tcgcctacct
      301 gtcgagcctg tgcgcagccg cctacacggc gacgcagtac gcgctgctga cctcgctgat
      361 ggcggtcggc atgcgcgtgt tcgcggcgag cggcggctgg ctggccgagc agctcggctg
      421 ggtctggttc tggggcggct cggcgctgct ggcgctgccg gggctgctgc tgctgtggtg
      481 gctgcagcgg cagggcgtgg cgcgggcacc ggcggcggcg caggaggcgg gggcgggctg
      541 agtccggccg cctcgcgaga ttccgggggc aggccgcgca cgcggcagcc cagaaacacg
      601 acgggccggc acccctgcag gtaccggccc gtcgctcgcc gcgggaaagg ctgcggatca
      661 ggtcgccagg ctcttctgcg ccggcagcgc ctcgccggtg tgcacctcgt cgcggccggt
      721 cagcaggcgc aggccggcgc ggtagaaggt gaccgcggcc gggctgtcgt gctcttcgag
      781 cagcgcgccg agggcctcgt aggtatcggc catcggcgtg atcttcaggc tggcttcgag
      841 gtaggcgcgg gccttgtcga gcttgtgggc gcgggcggcg aggcgcccga gcgtcagcag
      901 cagccaggga ttgtccttgt gcaccgacag ccagccctcg gcggtcgcga gctgcgcgtt
      961 cgggtcgccg cggccgagct ggccgtaggc gatgcagagc gggttgctcc acttctgcgc
     1021 gagcgcggcc ttcagcacct tctcggcctc ggccgggtcg tgctcgcgca ggctgccggc
     1081 gtactcgatc agcagggttt cctcgttgcg cagcgcgcgc ggcacgccgc tccaggcgcg
     1141 ctgcacgtcg gcggcgacgc cgcggcgcgt ggtactggcg aggcgttcgc ggtgcacctg
     1201 gatctggccg tcgtggaagc cggactcgtc gagcagcttg cgcttgcgca gctccggcag
     1261 caggcgcagc gcgtggtccc agtcgccgat gcggctggcg atctcgagct gcagctccag
     1321 cacgcgcggc tggttcgggg cgagcgtctg cacgtcgtgc agcaccttgc gcgcgccctc
     1381 gacgtcgtcg tcctccagca gcatctcggc gcgggcgatg ccgacgatcg cgaccgcatc
     1441 gcgcggcagc ttggcggcct gctcgaagta gcgctcgcgg cggtccttgg cgccgaggcg
     1501 gtgcgcggcg cgcgcggcac cgacgaagta cagcgcgcgg ttggtctcgg cgagcacgat
     1561 gcccttctgg aacgcgctct cggcggcctg caggcggcct tcctccagcg ccttctggcc
     1621 gagggcgagc aggcgcgcgg ccttgcgctc gcgccggcgc gtgctgatcg cgcgcagcgt
     1681 gcccggcatg cgcagcaggc ggccgaggta gcggatcagc ttgtagagga tgaagaagcc
     1741 gagcacgccg atcaccagcg ccgcggcgac cgaggtctcg atggtccagt tgccgacggt
     1801 gatcagcacg tagccggcgt cctggcgcac cagccaggcg aggccgaccg cggagaacag
     1861 cgcaacgatg aggccgatca ggaagctcac ggcttgacct ccccggccgg ggcggcttca
     1921 ggcgtggccg ccggggcggg ggcagcggcc ggggcgggcg gcgtcaccgg gccgacggcc
     1981 gacttcaccg gctggcggcg cgcgaggatt tcgttgaagg cctgcacggt gccggtcaga
     2041 tcgggcaggt agggctggaa ctgcgtctgc gcgaggccgt cgacttcctt ggccatcgcc
     2101 tgcacctgcg catcggcggt gtcgaagtat tccttcagca ggccctgcgc gagctgcgcc
     2161 gactgctgga aggcgacggc gtcctcacgc agcagcgcga cgcgcatgcc ttcgaggttc
     2221 agcttcaggt tctcgtgcag gaagtaggat tccttcggcg acatcagcgg ctgcgactgc
     2281 gtgcgctcgt gcttgatgac gacgatgtcc ttgatctgat tccaggcgtt ggtcgcgagg
     2341 cgctcgtacc acgggtcctc gctgtccggc acgctgttct cgagctgcgg cttgacgcgt
     2401 gccttcaggt tgtcgacctg gatgtcgggg cgcacgcgca gcgtcgcggc cttgccttcg
     2461 agctcgatga tgcgcgccga cagcgccgcg tgatccggca ggcgcacgcc gcgcacggtg
     2521 gcgacgttct cggcgagcat gcgctgcacg ctcgcgaacg cgagttcgtt ggcggcgccg
     2581 aggcgctgct cggccagcga cagtgcggcg agcgcggcct tcgggtcctt ttcgagcgcg
     2641 agcttgtggt tggcgatgcg cagcagatag ccgacttcgg acagcgtcag cgcgttcacg
     2701 tcgccgcgcg cggcggcggt cttggcggcc tcgacctcgt cgaacaggcc gcgctggccg
     2761 agctgcagcg tctgcacgcg gtcggcgagc gcggtcaggt cggcggcctg cttcttctgc
     2821 acgtcggcga tctggccgga gaactggccg gtcagcgagg acttggcggc ggcgagctcg
     2881 tcctgcacgg acttgaccac ggcctgcatg tcggcgagct gcttggcgcc ctgcgcctgg
     2941 ctgtcgctga tcgccttctt cacgcgcgca tcgagttcgg cgtcgtgctg gatcgtggcc
     3001 tgccggccct gccaccactg gtacccgacg taggccgtgc cggccgccgc ggccagcgcc
     3061 accgccagcg cgatgccgcc gagcgggctg ctgccgctgc gcaccggtac ggcctcgacc
     3121 gctgtcgcct tggccggcgc cgccggtgct gccttgccgc ccgccggctt gtcgtcagcc
     3181 ttcggcgcgc ccttgccgcc cgtcaggatc ggcgcctgcg gtgatggctt gcccggttgg
     3241 gtcgtcttgt ccgtcatgtc gggtcctctg gtggatcgct ggtgggatca cgcgcggtga
     3301 tcgcaggtgg ttccggtgtg gttgctggag tgatcgtgtg ggtcgtgtgc tctgtcgtgc
     3361 gcgccgaatg ccacccggcc agcgcagcca cgatcgccgc tgcatcggcg cccgtggcga
     3421 tcacgggcgc gtgcgaccag cccagatcgc gggccagacg ggcggcgcgc gggctgacga
     3481 ccagcagggg cgtcgagagc agcgattctc gcccgcgcgc gtcaagcaga tcaaagagat
     3541 tcgtcaagga ttcgttgctg ctcgccgtga cgatgtcgat cgccccgtcg cgcagccagc
     3601 cggcgaggcg ctgcgggtcg acatcgggcg gcggcgcgcg ccggtagagc tcggcgatca
     3661 cgacctcggc gccgcggcgc ccgagttcgg cgccgagcag ctcgcgcccg ccctcgccgc
     3721 gcaggatcac gacgcgccgc ccgcgcacgc agccggcggc aagctccggc gtggcgagca
     3781 gctcctcgct gcgcagcgcc cccggcgtgt cgtgcgcgac cggcacgccg agctcgcgca
     3841 ccgcacgcgc actcgccccg ccgatcgcca gacagccgac cccgtccggc cagccgccgc
     3901 gcgcggccag ccaggggcgc agcagcgcga cgacgttggc gctggtcacg atcgcgagcg
     3961 cgcagcccgg cagccggtcg agcgcggcgc aggccgcgga cgggtcctgc ggcggcagga
     4021 tcgccagcgc cgggaagctg cgcacgaacc cgccggcggc ctcgatcgca ccggcgagcg
     4081 cgccggactg cgccgccggg cgcgtcacca gcacctgcag gcccttcagg gccgtgctca
     4141 tcgccgtgcc gctccggcgt tcagggctgc gcgccgaaca cgcgcgcgag gatcacgtcg
     4201 gcaccgagcg cgagcaggtc ctcggcgagc tgcacgccga gcgcttcggc ctcgctcgcc
     4261 ggaccgcgcc gttcggcgcg cagcgtgcgg ctgccgtccg gctcgccgac caggccgcgc
     4321 agccagagct ggtcgccgtc gaggatcgcg tgcccggcga tcggcacctg acagccgcct
     4381 ttgaggcgcg tgttcagcgc acgctcggcg cgcaggcgca ggtgcgtgtc ggcgtcgtcg
     4441 agcaccgcga tcagcgcccc ggtgcgcgca tcgtcgcgcc ggcattcgat gccgatcgcg
     4501 ccctgaccga tcgccggcag gctggtttcg ggttcgagtt cgcagcggat gcggtcggcg
     4561 aagccgagac gcttgaggcc ggcggcggcg aggatgatcg cgtcgaactg gccggcatcg
     4621 agcttcgcga gccgcgaatt gacgttgccg cgcaggctgt cgatgacgat atccggccgg
     4681 cgcgcggcga gctggcactg gcggcgcagg ctggaggtac cgacgcgcgc gccctgcggc
     4741 aggtcttcaa agcgcgcgta cgcgttcgag acgaaggcat cgcgcgggtc ctcgcgctcc
     4801 aggatcaccg gcaggtgcag gccatccgga aattcgaccg gcacgtcctt catcgaatgc
     4861 acggcgatgt cggcgcgccc gtcgagcagc gcgacttcga gttccttcac gaacaggccc
     4921 ttgccgccga ccttggcgag cgggctgtcg aggatgcggt cgccctgcgt gctcatgccg
     4981 accagctcga ctgccagccc cggatgcgcg gcgcgcaggc gcgcggcaac gtgctcggcc
     5041 tgccacaggg cgagcggact cttgcgggtg gcgatgcgca gcgtcggagt gtcggtcatc
     5101 gttggcggcc ggtcgcgaaa gtgcgggagg gtacgcaagg gggcggggga agtcatcctt
     5161 ccaggtcaag gcggatgcca ttgcggccgg ccggtgccgc gagcgccccg gcgcggcgcg
     5221 tggcgagcgc ccggaagggc gccgagatgc gttcgtcggc ggcgatgcgg gaccagtagc
     5281 cgatggcggc cgcgatcagc tgcggcccgg ccggcaggac cggcggcggg ccgtcccctt
     5341 cgcgcacgct gccttcgcga tccggcgcca gcgccatcgg cagcatgtcg tagaccggcg
     5401 ccagccgcag gccggccggc ccggggatca gcgccagatt gccgaggtga cggtcgtggt
     5461 tgccgatcag cgcaccgaag gcatcgagcc agtcgatgcg cgccgcttcc gcggccggca
     5521 gccggccggt ggcggcgagt ccggccgcga cccgcgacca gccgctgccg tggccgacgt
     5581 attcggcatc gagcgcggcc aggctcagca ccggccgccg gccgtgtgcg ccgcagcggt
     5641 cgaagcgttc gacttcgaga aaggtgcggg cctctccgac gacgatgcgc gccgcggggg
     5701 ctgcaaaccc ctgcgcggcc agggtgtcca gggccagcgc ctcgcagacc agcagatcgg
     5761 cccagcgctg cccgcctgcc gtgctgcgtg gcggcgagaa cttcaccagc accggccggc
     5821 cgcccttgtc gccatccgcg accgtgaccg cgaacttcgg ctgctcgccg ccggcactcg
     5881 atccggccgg ctcgccggcc agcacggcag cggcgagctg cgcataggtg tcggcggtcg
     5941 ccggcagcgg tgcctgcgtc cgctgccgtt gccagcgcag caggctctcg tcgccgatca
     6001 gcagctcgcc ggccagatcc tcgccggccc gcgccagcgc gatcagcagg tgatcgtcat
     6061 gccagtgcag caggttgtcc ggcagcccga gttccggccg ctgcttcggc agcagccggc
     6121 cgagaaagcc ctgcggccgg atgtcccaca gaaaataagg aaccccggca tgcacgccat
     6181 cggcgttgtc gccccactgg taggcggcga gtgtgtcgtc gacggcctca caccagcagc
     6241 cgccatcgct cagcgcatgc agcgtgccga ccgccagcac ccggccgtcg gcgtcgatcc
     6301 ggtgaatcgg caggtggctg ccgagcgagg gcagcgagcg ggccagcgca tagcgcgtcg
     6361 cgcgtgcccg gccgatgcgc agcacctgcg cagcgctacg tgcaagccgg cgcgacagcg
     6421 tcgactggct gatgccaagc cgcgcgcaca gcgtctgcgc cggttgcggc ggcagaccgg
     6481 cgagtgcgtc gatcaggtca tcgggcgcgg gagcgggcat cgaagtggag tgggctgcat
     6541 ggattcggga cgggattcca gcagcccggg cgccggcctg acaaggactt gccgctgacg
     6601 gggccggatg catgcatcga tgatccggcc tcctgcgcgg cctcacccca ccgccccgac
     6661 ctccccgagc gccaccatca gcatcgacga atcgacgcgc ggggcggact tgaggtcggt
     6721 gagcaccgcg gcgaggcgtt cgaccgcggc gtggttgtgg gcgagccagg cgtcgatcca
     6781 cggctgcggg tgctcctccg gcccgcgctc ggcgacgacg gcggccgcga ggcggcgctg
     6841 ctgggtgtag aggttgtcga gcagcgcgct gcgggcgcgg tgctgccagt ggttgccggc
     6901 cccgaggcgg tcgatggtcg cggccagcca gtcgagttcg agcgcgtcga tcagcgtgaa
     6961 gtacaccagc gccacctgct cggcggcgat ctgcgcctga ttggcgacct cgatgatgtc
     7021 gagcgtgcag tacagcgccg gcaggctcgc gacccactcg gcggtctcgg ccggcacgcc
     7081 gtggccttcg aggcgcgccg cctcggcctg cagcgtcgca ccggcacggc ccggcatcag
     7141 gttcttcatc gacagcaggc gcgtgatgtc gggcgcgaag cgcgcgacct cggcctcgat
     7201 cgcgatcggc ggccggcggt tgcgcagcag ccacagcgag gcgcgcatca gcagcgcctg
     7261 cgtgtccgag atcatgtcga gctgcagcgt cgcggcggcg cgattgtcga gcgcgtcgat
     7321 cgctgcccac agcctggggg cctcgaacat cgcgcgcgcc gcggcgtagg cgcgggcgat
     7381 gtccggcgag cgcgcgccgc actgctcgtt gacgcggaac gcgaaggtcg agcccatgtg
     7441 gttcagcatc gagttcgtga tcgccgtggc aatgatctgg cgccgcagcg ggtggcgttc
     7501 catcgcctcg gcgtagcgct cgcgcagcgg ctcggggaag tagccgcgca gctcgcgttc
     7561 gaggtagggg tcctcgggca cgtcggaatc gagcagctcg tcgtacagcg cgatcttcga
     7621 gtacgcgagc agcacggcga tttccggcgc cgtcaggccc tgaccggcct gctcgcgttc
     7681 ggcgatctcg tcatcgggcg gcaggaactc gatgcgccgg ttcagccggc cggccttttc
     7741 gagctggcgg atcacgcgcg cgtggtcgcc gagcagatcc ggcgcctcgc gcaccgcgat
     7801 cgagatcgtc tgcggctgca gcacgttctg gcgcagcacc agacgggcga cctcgtcggt
     7861 catcgtcgcc agcagctcgt tgcgctgctt cagcgtcagg tcgccggcgc tgacgatctg
     7921 gttcagcagg atcttgatgt tgacctcgtg atccgagcag ttgacgccgg ccgagttgtc
     7981 gatggcatcg gtcaggatgt ggccgccggc gcgcgcgtac tcgatgcgtc cgcgctgcgt
     8041 gaagccgagg ttgccgccct cgccgaccac cttgcagcgc agctcgctgc cgttgacgcg
     8101 gatcgcgtcg ttggcgcggt cgccgacctc ggcgtgcgtc tcgtcggcgg ccttgacgta
     8161 ggtgccgatg ccgccgttcc acagcagctc gaccggcgca cgcaggatgg tgttgatgag
     8221 ttccgccggc gacaggcgcg tcgcgtcggt gccgaggatg gcgcgcatct cggggctgag
     8281 cgggatcgac ttggccgtgc gcggccagat gccgccgccg gccgagatca gcgcggcgtc
     8341 gtagtcggcc caggacgagc gcggcagcgc gaacagccgc tcgcgctcgg cgaagctcgc
     8401 ctcggcgtcc ggcgacgggt cgatgaacac gtggcggtgg tcgaaggccg cgatcaggcg
     8461 gatgtggcgc gacagcagca tgccgttgcc gaacacgtcg ccgctcatgt cgccgatgcc
     8521 ggcgaccgag aagtcgacgt tctggatgtc cttgccgagt tcgcggaagt ggcgcttgac
     8581 cgattcccag gcgccgcgcg cggtgatgcc catcttcttg tggtcgtagc cggccgagcc
     8641 gccggaggcg aaggcatcgc cgagccagaa gccgtattcg agcgccagac cgttggcgat
     8701 gtccgagaag gtcgccgtgc ccttgtcggc ggcgaccacc agatacgggt catcggcgtc
     8761 gtagcgcacg acacgcgccg gcggcacgac ctcgccgtgc acgaggttgt cggtgatgtc
     8821 gagcatgccg cgcatcagcg tcttgtagca gtgcacgacc tcctgcatca gcgcctcgcg
     8881 ctcggccggc gggcgcttga cgacgaagcc gcccttggag ccgaccggca cgatcacggc
     8941 gttcttgacc tgctgcgcct tcacgagccc gagcacctcg gtgcggaagt cctcgcgccg
     9001 gtccgaccag cggatgccgc cgcgtgcgac gcggccgccg cgcaggtgga tggcctcggc
     9061 gcgcggcgag tacacccaga tctcgaacat cggccgcggc agcggcagct ccggaatctt
     9121 cgccgggtcg aacttgaacg acaggtattc cttcggcccg ccgtcggtgc cgggctggcc
     9181 gggctggctg gtctggtaga aattggtgcg cagcgtcgcg aggatcaccg cgaggaagcg
     9241 gcgcaggatg cggtcctcat cgagcacggc gacgttttcg agcgccgact cgatgccgcc
     9301 ggcgagctgt tcgcagcgcg ccgcgtccgg gtgatcggga tcgaagcgcg cgacgaacag
     9361 cgccgcgagc tggccggtga tcggcgcgtg gcgcgccagc gtctcctcca tgtagctctg
     9421 cgagaacggc acccgcgtct gcagcaggta cttggcgtag gcgcgcagca cgacgacctc
     9481 gcgccagccg agcccggcgc cgagcaccag ccggttgaag ccgtcgttct ccagcgcccc
     9541 ggcccagacc tgcgcgaagg cctgctcgaa cagcgcgcgc acgcgcccga catcgacgtg
     9601 gatgccggcg ctctcgcgca ggtcgaagtc gagcacccac agcggcccgg cctcgccacg
     9661 cggctcgacc tcgtaggggc gcgtctccat gaccgcgagg cccatgcgtt cgagcatcgg
     9721 cagcacgttg gtcagcggca cgaccggcgc gcggccgtag agcttgaagc gcagccagcc
     9781 ctcgggcgat tcctgcggcc ggtacaggtg caggccgagc ggcgcgccgc cgacgacggc
     9841 ttcgagccgg tcgatgtcgc cgaccgcggc gcgcggcgtg aagtcctcct ggtacgcggc
     9901 cccgaaggcg ccggcgtagc ggttgtacag cgcgatgccg ccgccctcgc cgtgtgcctc
     9961 gatcagcgcc gcgcgcaggc cgtcctccca cgacagcatc gtctcgcgca ggcgtgcgcg
    10021 gatttcctcg acgctgtagg ccgggatgtg gcccggcgtc gtgcggatcg tgaacagcac
    10081 gcgcgccagc accgattcgc cgaactcgac ccagaagtcg gcgccgtgac cgttcagcgc
    10141 cgcgatcagc acgtcctgca tgcgcttgcg cagatcggtg ttgtagcgct cgcgcggcac
    10201 gtagatcagc gccgtcgcgt agcgctcgta ggcatcgatg cggatgaaca cgcgcaggtg
    10261 ctggcgttct tcgaggtgca ggatgccggt ggcgatcggg tacagctcgt cgacgctcgc
    10321 ctgcagcagc tcgtcgcgcg gataggtggc gaggatgtgc ttcagcgcct tgccggaatg
    10381 gctgaccgcc ggcagcccgg cgcgctcgat caccgcccgc accttggcgc gcagcaccgg
    10441 aatcccgaac ggactggtct ggtaggcgct cgacgtatac aggccgagga agcgccattc
    10501 gccgatgacc cggccggctt cgtcgaagcg cttgatgccg aggtaatcga gctgcaccgg
    10561 ccggtgcacg gtcgagcggg cgttggcctt ggtcatgacc agcagcaccg gtgccagcgc
    10621 caggcgccgg tgctgcggcg gcagcgcgtt gaagcgcgtg tcgtgatgcg cggcggcccc
    10681 gcggcgcagg ccgagcccgg tgcccggctc cgggtgcagc accagctcgc cgtcgagttc
    10741 ttcgagccgg tagccgctgt agccgagcag cgtgaagtgg ttgtcggccg cccagtcgag
    10801 gaaggcgcgc gcctcctcga cctccgcgac cggtaccggc agcttcgccg catcgagctc
    10861 atcggcgatt tcattgaggc gcgccagcat gcgcggccag tcctcgaccg cggcgcgcac
    10921 gtcatcgagc acgcgcgcca gatcgtcgcg cagcgtttcg aggcgggccg catcggtctc
    10981 gcggtcgatc gcgaactgca tcaccgcgag ctgcacgcca tcgccggccc cggtctcggc
    11041 caccgacagc ggctcgccgt cctcgtcgta ggcgatggcc agcaccgggt ggatcagccg
    11101 gtgcacggtg aagccgtggc ggttgagttc catgctcacc gaatcgacca ggaacggcat
    11161 gtccggcacg accagctcga tcgccgtatg cgtcgagcgc cagccgtgct cgtcgtagcc
    11221 cgggttgtag acgcgcaggc cgatctcgcc ggtgcgtgtg cgccgcagca ggttccagtg
    11281 cgagagcacg gcgccgtaga ggtcttcggg atcgtcgttg atcaggtcct cgggcgcgac
    11341 gcgcgcgaag tacacgcgcg cgaactgcgc ggcgaccgca ccgccggccg gcaggcgctc
    11401 ggtgagccgc gccgcgacgc gctcgatcag ttcggccttc ttgtccgctg ggctgctgct
    11461 catggtgggg aacctcggat tgcgcttctc gggagggcgc gcccgtcggc gggcgtctgc
    11521 ttggtgtcac ctgcgccggc gccgggttcg gggaacgcgg acggcgtacc gggcaggggg
    11581 tggtagcggg agtggcggga agtctagacg gcgggggaag gcagggcaaa cgcatcttcc
    11641 catcctatga aaacgccata catgacctgc actt
//
LOCUS       DAOQIE010000091        11667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2723_length_11667_cov_7.935498, whole genome shotgun sequence.
ACCESSION   DAOQIE010000091 DAOQIE010000000
VERSION     DAOQIE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_11667_cov_7.935498"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..856)
                     /locus_tag="PK144_07380"
     CDS             complement(98..856)
                     /locus_tag="PK144_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65587.1"
                     /translation="MPNLLAWQAATDWKTSPISLPANIEGFEMIAGPAPDPSWTGSPM
                     VAYLGPSGERRIWYDGVMCQQMGSYASMFVLLHEIAHHTLLHTAQPIVPTLLLSAAQQ
                     SMGREAQADNVACREAVRFFPLEAPEVLDAIYKYWAAAQGSGGSSHPSDAQRAVNVYQ
                     QWQSYLSTARCDILVHNDPLTPRDAIVKLLGVLQVNPVLVDPLIHQAELTGSLRLNYD
                     GNGYFYGQVINFIRAVITDARQNGYPLMIDFAMR"
     gene            complement(1052..1864)
                     /locus_tag="PK144_07385"
     CDS             complement(1052..1864)
                     /locus_tag="PK144_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HNF65588.1"
                     /translation="MNEALHALETGARLGYLARAVVYFMIGTLALMAGLGYGGETEDP
                     KDALRDLFRQPFGEVLVIAAAAGLAGYGLWRLLMAVRDPEHALYADEGRAQRIGRRLG
                     YVGSGLANLSLAAFALSLQFPRLVPFGHGGSGDGTRDWTARLLRVPAGEWLVAGVGVV
                     FLITAAVFAVRAWKAGFCKTLAPEAQRPLIVNLCRIGIGARAVVFALIGVFLIVAAWR
                     ADPREARGLGASLAALREQPAGEWLLAAVGAGLVAFAFFSLIEARWRRIRTQ"
     gene            2098..3888
                     /locus_tag="PK144_07390"
     CDS             2098..3888
                     /locus_tag="PK144_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNF65589.1"
                     /translation="MHRILVLLSAFGLAAPALAGAVDDARLRDAAQESGNWLSYGRDY
                     GQQRFSPLTQINRETIGRLVPKWIYQTGTTASFQATPIVVDGVLYLSMPFDHVAAIDA
                     ASGRELWRYEHQRRTPKMCCGPASRGVAVAYGKVFIGTVDARLIALDAASGKPVWDVA
                     VATDEGPTESVGQLDAGKAATRPSATGSTGIGVNMAPLVVDGKVIVGITGVGYGLHLE
                     AETPNAPVGTVVGFAGRYGRPGFYAAFDAQTGRRVWQFDTVPASGWEGSFRSTTPDGV
                     ALPRDPASEQAELGKYPDAARYGGGSAWTTPAYDAVSGLLYVGIGNPSPQMDDRSRPG
                     DNLYTVSLAALDAGTGTLKWHFQQVPHDLWGYDVASPPLLFELPTDKGPVAAVGQASK
                     LGWFYAHERRSGALLFKSEAFVPQENLFAAPTAEGTRIAPGAVGGASWSPVAFDPASG
                     LAYVAAAHIPIRYTLREIAGTKGGEALRYTELKPVDEPNWGTLTAIATREGGRIRWQQ
                     RTDKPLIGGVLATAGGLVFTGEGDGFFDAFDAATGERLWRFQCGAGVNAPPVTYEVDG
                     VQYVAVAAGGNSLFGYKSGGALIVFALQRD"
     gene            complement(4034..4552)
                     /locus_tag="PK144_07395"
     CDS             complement(4034..4552)
                     /locus_tag="PK144_07395"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNF65590.1"
                     /translation="MPAVHRPALAVLLALVASGATAAPDLPKRKPGLWETTVSMPAMP
                     GGAMPAIKMCIDEKTDDLVGGPNGVGRENCEQVDIRREGARYVIESKCRFGETKTHTT
                     GWFEGSFDSDYKGELNTEYSPPMQGMKNGKVLIAGRWLGACPADMQPGDLLMPNGMKV
                     NPSKMPQGQPKP"
     gene            4833..5357
                     /locus_tag="PK144_07400"
     CDS             4833..5357
                     /locus_tag="PK144_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNF65591.1"
                     /translation="MKTTGITLLIGIGSEARGDDAAGLEVARLLSANPPPGWRIEQAT
                     GEATDLIMRWVGAERVIVVDAAQADWPAGTIVRLDGLRAVLPAQTRQTSTHATGLATA
                     VRLARALGLLPSELVLLAVCGECWCLGTGLSPAVAAATRHLAARLTGGEFPAAAHAWP
                     DRPADGPRPTGSGL"
     gene            5386..6483
                     /locus_tag="PK144_07405"
     CDS             5386..6483
                     /locus_tag="PK144_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF65592.1"
                     /translation="MQRLSLAGLAVLLHVLWADRREIIGPRLRDGAITLGILHRLEDL
                     PVGWSDTAQEAAQYRVVPRADSARFGHVTGPLSWKRWLFLPEEVLFRAVRDDQDGGFT
                     LSATPTAPLRAFLGVRGCDLAAIAIQDRIFAHDPAYCARREATLIIAINCLEPGGTCF
                     CASMETGPQARGAFDLRLTEWLDAGTPSYLVESGSAAGRKLLARLPIEACTPEDQARV
                     EQALAQAATRMGRQLDTGGLAERLAARPGHRHWQQVAARCLGCANCTLVCPTCFCSTI
                     EDRTDLSGTTAERVRRWDSCFDLEHSYIVGGNVRADPAARYRQWLLHKLAYWHTQFGT
                     SGCTGCGRCITWCPVGIDLTVEAAAVAGSEA"
     gene            6611..7093
                     /locus_tag="PK144_07410"
     CDS             6611..7093
                     /locus_tag="PK144_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNF65593.1"
                     /translation="MVEIAGLDRMLLEHPFFAGMAPEQAAFVAGCGRNVVVEAGAWLF
                     REGEAADQFYLLRHGRVTLELRAPGREPLIFDTLGEGDIVGWSWLLPPFCWSMDARAQ
                     TLVRVVALDAHCLRDKLDSDPALGYALLRRFVPVMGRRLQAARLQLADLYARPQGGRG
                     "
     gene            7093..7950
                     /locus_tag="PK144_07415"
     CDS             7093..7950
                     /locus_tag="PK144_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNF65594.1"
                     /translation="MNATPGGADPALWLPEPWRIERVRRELSDTFTLELQPTAGGAPL
                     RFLPGQFNMLYAFGLGEVPISISGDPEADGPLVHTIRAVGPVTEAMRRLVPGDVLGVR
                     GPFGSCWPVSGAEAEGCDLVIVAGGVGLAPLRPAILHVLRHRQRFGQVCIFYGARTPA
                     DILYRHELRRWRGQFDLLVDVTVDRATQDWAGRVGVVPRLVEGGGYDPQHTLALVCGP
                     EIMMRHTARALLARGVRRERIHVSMERNMKCAVGLCGHCQFGPHFVCRDGPVFRFDAI
                     EALMTVDGL"
     gene            7998..8738
                     /locus_tag="PK144_07420"
     CDS             7998..8738
                     /locus_tag="PK144_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNF65595.1"
                     /translation="MFKFASCDGCQLSLLDCEDELLAVCEAVEIAYFPEASSAMQPGP
                     YDIVLVEGSITTAHDRERIRRVRRSARRLVTIGACATSGGIQALRNFRDVAEFAHLVY
                     ARPEYLDTLAESTPIAAHVPVDHELRGCPIDQRQLLALLDALLRGRRPSIPSAAVCTE
                     CKRAGQVCVTVAQGTPCLGPVTQAGCGALCPGFARGCYGCFGPKEAPNTDALAVKLGE
                     LGLDPPAVRRFWHTFNAGAGAFRRAGGG"
     gene            8756..10060
                     /locus_tag="PK144_07425"
     CDS             8756..10060
                     /locus_tag="PK144_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNF65596.1"
                     /translation="MDGERTRRIEVGTLARVEGEGALTLRVRGDRLLDVQLRIYEPPR
                     FFEALLRGRDFREAPDISARICGICPIAYQLGAAQAMEDACGVQVGAGLRALRRLIYC
                     GEWIESHALHVFLLHLPDFLGMPDAVALAEAGHRDTVQAALRIRKLGNALMRVIGGRE
                     IHPVNLKVGGFWRAPDAEALQALLPDIGQATDAMLRQLDQLTRLAFPDFTVDCEFLAL
                     RHDEEYAITEGRLVSSRGIDLPLEGYDGLLTETHVRHSTALQSQRSDGGTPVHVGPLA
                     RLHLNRDRLPPELARLAGQIGLEPGCRNPFKSILARVIEVLYALGEARRLIDGYRVPE
                     APAIDCPPRAGTGYGATEAPRGLCYHRYTLAADGVITDAKIVAPTSVNQRQIEADLHE
                     YAASRLDQSDERLRHGCERLIRSYDPCISCSTHFLTLNIENG"
     gene            complement(10247..10552)
                     /locus_tag="PK144_07430"
     CDS             complement(10247..10552)
                     /locus_tag="PK144_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65597.1"
                     /translation="MEALDLNKTDLRRTTSYQLHRLALLLVDRLDREQQRANLVAEVR
                     KWRLRRRMRMIVSELLSRRSLDEVLSMAAASASDAHPQERSGELSRRYVEMIRSFHA"
     gene            11411..>11667
                     /locus_tag="PK144_07435"
     CDS             11411..>11667
                     /locus_tag="PK144_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005902497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNF65598.1"
                     /translation="MKTQTLLQSALATAIALGAGFGSIAHAADAPAEKCYGVVKAGKN
                     DCQTNTHACAGQAKADSQGDSWVYLPKGACEKITGGSLTKKA"
BASE COUNT         1750 a         4033 c         4116 g         1768 t
ORIGIN      
        1 gtgcagctcc gctgcgcacc atcagcgtta gaaatcacat ccgccgctca cgccgcgcag
       61 caaacgcaca ccctgcaact gtgcgcggtc gccccgttca tcgcatcgca aaatcaatca
      121 tcaatggata cccattctgc cgggcatccg tgatgacggc gcggatgaaa ttgatcacct
      181 gaccatagaa ataaccgttg ccgtcgtaat tgagccgcag gctgccggtg agttcggcct
      241 gatggatcag cggatcgacc agcaccggat tgacctgcag cacgccgagc agcttgacga
      301 tggcatcgcg cggcgtcagc ggatcgttgt gcacgaggat gtcgcagcgc gccgtgctca
      361 gatacgactg ccactgctga tagacgttga ctgcacgctg cgcatcggac ggatgcgagc
      421 tgccgccgct gccctgggca gccgcccagt acttgtaaat ggcatcgagc acctccggcg
      481 cttccagcgg gaagaaacgc acggcctccc ggcaggcgac gttgtcggcc tgcgcctccc
      541 ggcccatcga ctgctgggcg gcagaaagca gcagcgtcgg cacgatcggc tgggccgtgt
      601 gcagcagcgt gtgatgggcg atttcgtgca gcagcacgaa catcgaggca tacgaaccca
      661 tctgctgaca catgacgccg tcataccaga tgcggcgttc accgctcggg ccgaggtacg
      721 ccaccatcgg cgaaccggtc caggacgggt ccggtgccgg cccggcgatc atctcgaaac
      781 cttcgatgtt cgccggcagc gagatcggtg aggttttcca gtcggtggct gcctgccagg
      841 ccagcagatt cggcatgatc gatcccccag tatccgtgac cggtttccgg ctgccccctg
      901 catgcagtcc gggtccgctg ccggcaagcg tagcgggcgg gtcgggtcag caacgaaagc
      961 cggcctgaac ggcaggggga tgcgcgaacg cgcgcgccgc tggtgtaccg gcggcgaagc
     1021 agggtatgag cggcagcagc cggctgcgcc ctcactgcgt ccggattctc cgccagcgcg
     1081 cctcgatcag gctgaagaac gcaaacgcca ccagtccggc gccgacggcg gccagcagcc
     1141 attcgccggc cggctgttcg cgcagtgctg cgagcgaggc gccaagcccc ctggcttcac
     1201 gcggatcggc gcgccaggcg gcgacgatca ggaacacgcc gatcagcgcg aacaccacgg
     1261 cccgcgcgcc gatgccgatg cggcagaggt tcacgatcag cggccgttgc gcttcgggcg
     1321 cgagggtctt gcagaaaccg gccttccagg cgcgcacggc gaagaccgcg gcggtgatca
     1381 ggaacaccac gccgaccccg gccaccagcc attcgccggc cggaacgcgc aacagccgcg
     1441 ccgtccagtc gcgggtgccg tcgccgctgc cgccgtggcc aaacggcacg aggcgcggga
     1501 actgcaggct cagcgcgaat gcggccagcg acaggttggc gaggccgctg ccgacatagc
     1561 cgagccggcg gccgatgcgc tgcgcgcggc cttcatcggc gtacagcgca tgttcgggat
     1621 cacgcaccgc catcagcagc cgccagagtc cgtaacccgc cagcccggcg gcggcggcga
     1681 tgaccagcac ctcgccgaac ggctggcgga acaggtcacg cagcgcatcc ttcggatcct
     1741 cggtctcgcc gccgtagccg agcccggcca tcagcgccag cgtgccgatc atgaagtaga
     1801 cgacggcacg ggccagatag ccgaggcggg cgccggtttc gagggcatgc agggcttcgt
     1861 tcatcgccgg cggactccgg gcagtcttgt cgttgtcgtc gtcgggccgc agcggggagc
     1921 gcagccggtg cgcagcagcg cagtgcgctg cgggcaccgg ctgtttcgag ccatgccccg
     1981 gcacgcggtt cagtcggacc ggccggggtg caagggctgg cggcgcgtgc cggcgcctgc
     2041 ggctacagtg cccggcacgt tccgggatgc cccgatcccg ccgcagaggg cagtgccatg
     2101 catcgaatcc ttgtgctgct gtcggcattc ggccttgccg cgccggcgct ggccggtgcc
     2161 gtcgacgatg cgcgcctgcg cgatgcggcg caggagtccg gcaactggct ctcgtatggc
     2221 cgcgactacg gtcagcagcg cttttcgccg ctcacgcaga tcaaccgcga gaccatcggc
     2281 cggctggtgc cgaagtggat ctaccagacc ggcacgacgg cctcgttcca ggccacgccg
     2341 atcgtcgtcg acggcgtgct gtacctgtcg atgcccttcg atcacgttgc ggcgatcgac
     2401 gccgccagcg gccgcgagct gtggcgctac gaacaccagc ggcgcacgcc gaagatgtgc
     2461 tgcggtccgg cgagtcgcgg cgtggcggtg gcctacggca aggtgttcat cggcaccgtc
     2521 gatgcgcggc tgatcgcgct cgatgccgcc agcggcaagc cggtatggga tgtggccgtc
     2581 gccaccgacg agggaccgac cgagagcgtc gggcagctcg atgccggcaa ggctgcgacg
     2641 cggccgtcgg caaccggcag caccggcatc ggcgtcaaca tggcgccgct ggtggtggac
     2701 ggcaaggtca tcgtcggcat caccggcgtc ggctacggcc tgcacctcga agccgaaacc
     2761 ccgaacgcgc cggtcggcac cgtcgtcggc ttcgccggcc gctatggccg gccgggcttc
     2821 tatgccgcct tcgacgcgca gacgggcagg cgcgtctggc agttcgacac ggtgccggcg
     2881 agcggctggg aaggcagctt ccgcagcacg acgccggacg gggtggcgct gccgcgcgat
     2941 cccgccagcg agcaggccga actcgggaaa tatcccgatg ccgcccgcta cggcggcggt
     3001 tcggcgtgga cgacgccggc ctacgatgcc gtgagcggcc tgctgtacgt cggcatcggc
     3061 aacccgtcgc cgcagatgga cgaccgctcc cgccccgggg acaacctcta tacggtatcg
     3121 ctcgccgcac tcgatgccgg caccggcacg ctgaagtggc acttccagca ggtgccgcat
     3181 gatctctggg gctatgacgt ggccagcccg ccgctgctgt tcgagctgcc gacggacaag
     3241 ggcccggtgg cggcggtcgg gcaggcgtcg aagctcggct ggttctatgc gcatgagcgc
     3301 cgcagcgggg cgctgctgtt caagtccgag gccttcgtgc cgcaggagaa tctctttgcc
     3361 gcaccgacgg cggaaggcac gcgcatcgcg cccggtgcgg tcggcggtgc gagctggtcg
     3421 ccggtggctt tcgatccggc gagcggtctg gcctacgtcg cggccgcgca catcccgatc
     3481 cgctacacgc tgcgcgagat cgccggcacc aaaggcggcg aggcgctgcg ctacaccgag
     3541 ctgaaaccgg tcgatgagcc gaactggggc acgctgacgg cgatcgcgac gcgcgagggc
     3601 ggccgcatcc gctggcagca gcgcaccgac aagccgctga tcggcggcgt gctcgccacc
     3661 gccggcgggc tggtgttcac cggcgagggc gacgggtttt tcgatgcctt cgatgccgcc
     3721 accggcgagc ggctgtggcg cttccagtgc ggggccggcg tcaacgcgcc gccggtcacg
     3781 tatgaggtcg acggcgtgca gtacgtcgcc gtcgccgccg gcggcaattc gctgttcggc
     3841 tacaagagcg gcggcgcgct gatcgtgttc gcgctccagc gcgactgaaa gtccgtcact
     3901 cctccatgtc gggtctgtct cctgctccct cgcgttttgc cgcgtgagga ggcaggcgag
     3961 gcccgacccg ggcgcttgcc ggaccggggc ctggccggcg ctgcggcacc cgggccccgg
     4021 gcgtgcgccg ggttcagggc ttcggctggc cctgcggcat tttcgacgga ttgaccttca
     4081 tgccgttcgg catcagcagg tcgccgggct gcatgtcggc cggacaggcg ccgagccagc
     4141 gcccggcgat cagcaccttg ccgttcttca tgccctgcat cggcgggctg tattcggtgt
     4201 tcagctcgcc cttgtagtcg ctgtcgaagc tgccctcgaa ccagccggtc gtgtgcgtct
     4261 tcgtctcgcc gaagcggcac ttcgactcga tcacgtagcg cgcgccttcg cggcggatgt
     4321 cgacctgctc gcagttctcg cggccgacgc cgttcgggcc gccgaccaga tcgtcggtct
     4381 tctcgtcgat gcacatcttg atggccggca tcgcgccgcc gggcatggcc ggcatcgaca
     4441 cggtggtctc ccacaggccg ggcttgcgtt tgggcagatc gggggctgcc gtggcgccgg
     4501 aggcgacgag ggcgagcagc acggcgaggg cagggcggtg aacggcaggc atggcggact
     4561 ccggaggacg gggacacgtg aggccgcgct ggcggcggca gatacgacca cgcagcagcg
     4621 tagccgtcac gcggcacgga cgaatcggac gcgcagcagc gctcgtgccc tccgccgtgc
     4681 aatgcgcagg cggcctcatt gcggctgccg gcagcgcgaa cccggggagc atcttccata
     4741 ttcatcaggg agttgcgctg catcaatgaa gcggctttca tgatccgcag tgcatggcga
     4801 ggcgcatcgg tcggcgctag ggttcaggtg ccatgaagac gacgggaatc acgctcctga
     4861 tcggcatcgg cagcgaggcc cgcggcgatg atgccgccgg tctcgaagtc gcccggttgc
     4921 tcagcgcgaa cccgccgccc ggatggcgga tcgagcaggc gacgggagaa gcgaccgacc
     4981 tgatcatgcg ctgggtgggg gccgagcggg tgatcgtggt cgatgccgcg caggctgact
     5041 ggcctgcggg gacaatcgtc cggctcgacg ggctgcgtgc ggtgctgccg gcgcagacgc
     5101 ggcagacctc gacacacgcg acggggcttg ccaccgccgt gagactggcc cgggcgctcg
     5161 gcctgctgcc gagcgaactg gtgctgctcg cggtgtgcgg cgagtgctgg tgcctcggca
     5221 cgggtttgag cccggccgtg gccgcggcga cgaggcatct ggctgcgcga ctcaccgggg
     5281 gggagtttcc ggcggcagcg cacgcatggc ccgaccggcc cgcagacggg ccgcggccga
     5341 caggatccgg tctctgatcc atccgcaagc ggagggcgat ccaccatgca gcgcctgagt
     5401 ctggccggcc ttgccgtgct gctgcatgtg ctgtgggcgg accggcgcga gatcatcggt
     5461 ccgcggctgc gcgacggtgc gatcacgctc ggcatcctcc atcgtctcga agacctgccg
     5521 gtcggctgga gcgacaccgc ccaggaagcc gcgcagtacc gcgtcgtgcc cagggccgac
     5581 agcgcgcgct tcggtcatgt gaccgggccg ctgtcgtgga agcgctggct gttcctgccc
     5641 gaggaagtcc tgttccgggc cgtgcgcgac gaccaggacg gcggcttcac gctgtcggcg
     5701 acgccgacgg cgccgctgcg ggcgtttctc ggcgtgcgcg gctgcgatct ggcggcgatc
     5761 gcgatccagg accgcatctt cgcgcacgac cccgcgtact gcgcgcgccg ggaggcgacg
     5821 ctgatcatcg ccatcaactg cctggagccg ggcggcacct gcttctgtgc gtcgatggag
     5881 accggaccgc aggcgcgcgg cgccttcgac ctgaggctga ccgaatggct cgatgccggc
     5941 acgccctcgt atctggtcga gtccggcagt gcggccggtc gcaagctgct cgcgcggctg
     6001 ccgatcgaag cctgcacgcc ggaagatcag gcccgcgtcg agcaggcgct ggcgcaggca
     6061 gcgacgcgca tggggcggca gctcgatacc ggcgggctcg ccgaacgcct ggccgcacgg
     6121 cccggacacc ggcactggca gcaggtggcc gcgcgctgcc tgggctgcgc gaactgcacg
     6181 ctggtctgtc cgacctgctt ctgctcgacg atcgaggatc gcaccgacct gagcggcacg
     6241 acggccgagc gcgtgcggcg ctgggattcg tgcttcgatc tcgaacactc gtacatcgtc
     6301 ggcggcaacg tgcgcgccga tcccgcggcc cgctaccgcc agtggctgct gcacaagctc
     6361 gcgtactggc acacgcagtt cggcacttcg ggctgcaccg gctgcgggcg ctgcatcacc
     6421 tggtgcccgg tcggcatcga cctgaccgtg gaagcggcgg cggtggccgg cagtgaagca
     6481 tgaggcagga gacaggacag accggcgcga atgattcccc ggcaggacga cggcagacgg
     6541 gcgggacgac ccgtaccggc tgccgctccc gccgctcctt gctgccgcca cgaacaccgg
     6601 agtcgcaggc atggtcgaga tcgcagggct ggaccggatg ctgctggagc atccgttctt
     6661 cgccggcatg gcgccggagc aggcggcgtt cgtggccggc tgcggacgca acgtcgtggt
     6721 cgaggccggg gcctggctgt ttcgcgaggg cgaggcggcc gatcagttct atctgctgcg
     6781 ccacggccgc gtgacgctgg agctgcgcgc gccggggcgt gaaccgctga tcttcgacac
     6841 gctcggcgaa ggcgacatcg tcggctggtc gtggctgctg ccgccgttct gctggagcat
     6901 ggacgcgcgc gcgcagacgc tggtgcgcgt ggtcgcgctc gatgcgcact gcctgcgcga
     6961 caagctcgat tcggacccgg cgctcggcta cgcgctgctg cggcgcttcg tgccggtcat
     7021 gggccggcgg ctgcaggccg cgcgcctgca gctcgccgac ctgtatgccc ggccgcaggg
     7081 cgggcgcggc tgatgaacgc cacgcccggc ggggccgatc ccgcgctgtg gctgccggag
     7141 ccctggcgca tcgagcgcgt gcgccgcgag ctgtccgaca ccttcacgct cgaactgcag
     7201 ccgaccgccg gcggcgcgcc gctgcgcttc ctgcccgggc agttcaacat gctctacgcc
     7261 ttcgggctcg gcgaggtgcc gatcagcatc agcggcgatc ccgaagccga cggcccgctc
     7321 gtgcacacga tccgcgcggt cgggccggtc accgaggcga tgcgccggct cgtgcccggt
     7381 gacgtgctcg gcgtgcgcgg accgttcggc tcgtgctggc cggtgtccgg ggccgaggcc
     7441 gagggctgcg atctggtcat cgtcgccggc ggcgtcggac ttgcgccgct gcgcccggcg
     7501 atcctgcacg tgctgcgtca tcgccagcgc ttcggacagg tctgcatctt ctacggtgcg
     7561 cgcacgccgg ccgacatcct gtaccgccat gaactcaggc gctggcgcgg tcagttcgac
     7621 ctgctggtcg atgtgaccgt cgatcgcgcg acgcaggact gggccgggcg cgtcggcgtc
     7681 gtgccgcggc tggtcgaggg cggcggctac gacccgcagc acacgctcgc gctggtctgc
     7741 ggcccggaaa tcatgatgcg ccacacggcg cgggcgctgc tggcgcgcgg cgtccgccgc
     7801 gagcgcatcc acgtgtcgat ggagcgcaac atgaagtgcg cggtcgggct gtgcggccac
     7861 tgccagttcg ggccgcattt cgtctgccgc gacgggccgg tgttccgctt cgatgccatc
     7921 gaggcgctga tgacggtgga cgggctgtga acgcagcgcg caataccgca aggaatccgt
     7981 cccggccgcg gctggccgtg ttcaagttcg cctcctgcga cggctgccag ctgtcgctgc
     8041 tcgactgcga ggacgaactg ctggccgtgt gcgaggcggt cgagatcgcg tacttcccgg
     8101 aagcctcgtc ggcgatgcag ccggggccgt acgacatcgt gctggtcgaa ggctcgatca
     8161 cgaccgcgca cgatcgcgag cgcatccgca gggtccggcg cagcgcccgg cggctggtga
     8221 cgatcggcgc ctgcgcgacc agcggtggca tccaggcgct gcgcaatttc cgcgatgtcg
     8281 ccgagtttgc gcacctcgtc tacgcccggc ccgagtacct cgacacgctc gccgagtcga
     8341 cgccgatcgc cgcgcacgtg ccggtcgatc acgagctgcg cggctgcccg atcgaccagc
     8401 gccagctgct tgcgctgctc gacgcgctgc tgcgtggtcg ccggccgtcg atcccgtcgg
     8461 ctgcggtctg caccgagtgc aagcgcgccg gccaggtctg cgtgacggtt gcgcagggca
     8521 ccccctgcct cggcccggtc acgcaggccg gctgcggggc gctgtgcccg ggcttcgcgc
     8581 gcggctgcta cggctgcttc ggaccgaagg aggcgccgaa taccgatgcc ctcgccgtga
     8641 agctcggcga actcggcctc gacccgccgg cggtccggcg cttctggcac accttcaacg
     8701 ccggcgccgg cgccttccgc cgcgccggtg gcggctgaag ccgcgggaac cggacatgga
     8761 cggcgagcgc acacggcgca tcgaggtcgg cacgctcgcg cgcgtcgagg gcgagggggc
     8821 gctgacgctg cgcgtgcgcg gcgaccggct gctcgacgtg cagctgcgca tctacgagcc
     8881 gccgcgcttc ttcgaggcgc tgctgcgcgg gcgcgatttc cgcgaggccc cggacatcag
     8941 cgcccgcatc tgcggcatct gcccgatcgc gtaccagctc ggggctgcgc aggcgatgga
     9001 ggatgcctgc ggcgtgcagg tcggggccgg actgcgggcg ctgcgccggc tgatctactg
     9061 cggcgaatgg atcgagagcc acgcgctgca cgtcttcctg ctgcatctgc cggatttcct
     9121 cggcatgccc gatgccgtcg cgcttgccga ggccggccac cgcgatacgg tccaggcggc
     9181 cctgcgcatc cgcaagctcg gcaacgcgct gatgcgcgtg atcggcggtc gcgagattca
     9241 cccggtgaac ctgaaggtcg gcggcttctg gcgcgcgccc gatgccgagg cgctgcaggc
     9301 gctgctgccg gacatcgggc aggccaccga tgcgatgctg cgccagctcg atcagctcac
     9361 gcggctggcc ttcccggatt tcacggtcga ctgcgagttc ctggcgctgc gccacgacga
     9421 ggaatacgcg atcaccgaag gccggctggt cagctcgcgc ggcatcgacc tgccgctcga
     9481 aggctatgac gggctgctca ccgaaaccca tgtccgccat tcgaccgcgc tgcagagcca
     9541 gcgcagcgac ggcggcacgc cggtgcacgt cgggccgctg gcgcgcctgc acctgaaccg
     9601 cgaccggctg ccgccggagc tggcccggct cgccgggcag atcggactgg agcccggctg
     9661 ccgcaacccg ttcaagtcca tcctcgcgcg tgtgatcgag gtgctgtacg cactcggcga
     9721 ggcgcgccgg ctgatcgacg gataccgcgt gcccgaggcg ccggccatcg actgcccgcc
     9781 gcgcgccggc accggctacg gtgccaccga ggccccgcgc ggcctctgtt atcaccgcta
     9841 cacgctcgcc gccgacggcg tgatcaccga cgcgaagatc gtcgccccga cctcggtcaa
     9901 ccagcgccag atcgaggccg acctgcacga gtacgccgcc agccgcctcg accagtccga
     9961 cgagcgcctg cgccacggct gcgaacggct gatccgcagc tacgacccgt gcatctcctg
    10021 ctcgacgcac ttcctgacgc tgaacatcga aaacggctga ggaaagccgc taaaaatata
    10081 atttttatac catcatatag cgtgctgccg cttccgcagc aggaacaggt ttctgatgac
    10141 cgggcggaga ccggtgcgca tcaccggaag cgatctgaca gggcagcgct ctgccggccg
    10201 tagcgcggga tacccgccgg ccctctgcgg gccggcagcg gggggatcag gcgtggaagc
    10261 tgcggatcat ctcgacgtag cggcgcgaca gttcgccgct gcgttcctgc ggatgggcat
    10321 cggaagcgct cgcggcggcc atcgacagca cttcgtcgag gctgcggcgg ctcagcagtt
    10381 cggacacgat catgcgcatg cgccggcgca gccgccattt gcgcacctcg gccacgaggt
    10441 tggcgcgctg ctgttcacga tcgagccggt cgaccagcag cagcgcgagc cggtgcaact
    10501 ggtaggacgt ggtgcggcgc aggtcagtct tgttcagatc gagggcttcc atggctcatc
    10561 ggtcctccgg catggggggt gaggaaatgt gaataaatct taatgacact agagtcatta
    10621 ctcaacgcac gtcctgtgca ttgcattgca gcatattcag cggaatttac aaatgtaaag
    10681 tgatgccgga tgcggcgtaa ggttgctgac cgccgccggc accctcctga aacattcatg
    10741 catcagtcgt gctaagtacc ggtacaaagt gaagaatctg agcagcgatg ccgtcggccg
    10801 gacccggttc catcgtggtc tggccgccat gcagggcggc cggcggcatt cggcaggctt
    10861 gccggaagat gctgtttcat cgggttttct tacacttttc cgcttgggtt cctgcgcggc
    10921 agccaaggct cacgcacgcg ctgcgccgct ccgtcaccgc cagcgcttgc ccggacaccc
    10981 ttgaaaaaaa atcatccgaa gccccccgca ccggaatccg ggctgcaggg cgcctgcacg
    11041 ccggatgatc cggaatgcac cgcctgaaag cgtggccatc cgccgctgca cgcggagcgt
    11101 gcttcccgga cggcaggggc acccgggggc aggtggctgc gcgtgaagtc agccgcgatc
    11161 gggcgcgtca gcccgcgaaa tcccgtggcc gtggcgggac ggagcgccgg acgcgaaccc
    11221 ggcaatccgc cggctcctga taaaagatga acataaacat tgacttgtct ggatgccttc
    11281 ccggtgcatc cggctggcac ggcgactgcg taaggggaat cgtccgcagc agcacgaaaa
    11341 ggccaacacg gccgctgcac ggatcctctc ccagaccctg aaccgttttc ccccatccgg
    11401 agattgcgcc atgaagaccc agaccctgct gcagtccgcg ctcgccaccg ccatcgccct
    11461 cggcgccggt ttcggcagca tcgcccatgc cgccgacgcg ccggccgaaa agtgctacgg
    11521 cgtggtcaag gccggcaaga acgactgcca gaccaacacc cacgcctgcg ccggccaggc
    11581 caaggccgac tcgcagggcg actcctgggt ttatctgccc aagggcgcct gcgagaagat
    11641 caccggcggc agcctgacca agaaggc
//
LOCUS       DAOQIE010000092        11631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2738_length_11631_cov_6.943417, whole genome shotgun sequence.
ACCESSION   DAOQIE010000092 DAOQIE010000000
VERSION     DAOQIE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2738_length_11631_cov_6.943417"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..400)
                     /locus_tag="PK144_07440"
     CDS             complement(80..400)
                     /locus_tag="PK144_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65599.1"
                     /translation="MAARARRRRPRTARRSPPTLRGLEPDRHPWLALGGHITWRLPWR
                     ADALLDVVAAGETTPQALLDGIGNLAAGKWDWAVPLDLRQRDFAGQRLEIDAGIAMVA
                     ALAG"
     gene            complement(449..976)
                     /locus_tag="PK144_07445"
     CDS             complement(449..976)
                     /locus_tag="PK144_07445"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF65600.1"
                     /translation="MSLLPPVTADRFVPAPLFRRLAAAVYDVLPLAGVLVMAEALATG
                     LGRLLLGAEGYGQHAGLAGNPWNTSFVFLVCFAYYGGFWVHGGQTLGLRTWKLRVQLP
                     GGGRIGWWHALLRFLAGLAWLVPGLGLYKALHAPVWMAIALSLACLIATLASRLPDRA
                     AGTEMVRLLRDGGGG"
     gene            1093..1446
                     /locus_tag="PK144_07450"
     CDS             1093..1446
                     /locus_tag="PK144_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNF65601.1"
                     /translation="MSIVADLMNLPGVIAAGEYAFRGDRYSYRGQLTEDQARMCTILC
                     RASTMGIVMQGRMAETALPGAGIRPRGWAMRGPEHAMCVVGNVFCVMKNQSGTMDSVL
                     ARLNTAMADVDMHLV"
     gene            1583..1969
                     /locus_tag="PK144_07455"
     CDS             1583..1969
                     /locus_tag="PK144_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNF65602.1"
                     /translation="MARLGNLLDQPGVLAAFEFRASGELLDHELRAGSPITTDILELL
                     ASMCAANVSICGMQARGWEKLTGMSGFSNVREFTLVGFDWSVVVSCAQREAQRAAGQE
                     TIAPYQGVVLINRDARYEAACKALEA"
     gene            1980..2315
                     /locus_tag="PK144_07460"
     CDS             1980..2315
                     /locus_tag="PK144_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNF65603.1"
                     /translation="MIKRLLAVDGVLAVCRFRDDGGMVESYGVGLDQDQLGKLSLVAH
                     EYRRMLQGHADLFSMFTNMRGWTPPRGWAVRGEKLTLCGVAGVVCILDNAEADYDEVL
                     KDMIEIAHW"
     gene            2538..4295
                     /locus_tag="PK144_07465"
     CDS             2538..4295
                     /locus_tag="PK144_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF65604.1"
                     /translation="MPSSFHRCLRALLLPVVLLAPISVPAAPAATEPVAAELRARLAT
                     PEALAGEALRERPLLARFYAARAGKPAWSEPRLLDALLAAVDKSPEDGLDAGDYHRAR
                     LATAIDAARRGRLDATGLAALDVLATDAWLALAQDLLSGRLDPQQLDKDWTLRPRSRD
                     LVAALNEALANRRIGESLAALAPAYPEYAQLRRGLAELRRQLAAGGWPTVGALAKGEK
                     IEPGQPHPRVREIRARLLATKEVEATTPVLAPVSVPVPAPAAPAAATTAAGGETAATP
                     AQPAAAEPAPVETVLAEVYDATLVEAVHRFQARHGLTEDGVIGAATVAAMNVSVRERI
                     AQVRLNLERWRWLPGDLGQRYIVVNIPGFELQVIERGRPVLEAKVVVGTQVKPTPVFT
                     GRMQQVVFAPYWNVPGTIASEELLPKLRKNPGEFVKTNMKVITGSGAVVDPTAVNWGE
                     YHPGNFPFRLRQEPGAANPLGRVKFIFPNRYDVYLHDTSKPALFERSQRTYSHGCIRI
                     SRPAELAEYLLKSDPRWNRAAIDAAMDAGRERAVPVPEPLPVHLLYWTSWVDAQGLLQ
                     FRADVYGRDLALRKALSAL"
     gene            4583..5797
                     /gene="flgL"
                     /locus_tag="PK144_07470"
     CDS             4583..5797
                     /gene="flgL"
                     /locus_tag="PK144_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNF65605.1"
                     /translation="MIRISTSYTQEQGVQYMRDAQKRVVDTQAELATGKRISSPSDDP
                     VGAARLIDLHHQIDSTARFQTNIAMATGRLATSDSILDEAGQLTQRARELVVQANNAP
                     LADSDRVSIAQELRQIRGQLLEHANSKDGNGDYLYAGYRTQTTPFIETTGGQVVYQGD
                     SGQRLLGIAPTRSVAVSDAGDSVFMNIRAGNGVFATTADAGNTGSAVIEPGDLLDPTA
                     FEHHRFQIDFQSSTDFTITDLSEVPPTGPGPVQTLPADGRIEFNGISVKLTGAPANGD
                     RFEVAPAPYQDAFTTLDKVITALETPVAATGAARTKFAQTMADAMQNLDQMHSNFLDT
                     RTQIGARLNGLETQESANSEFSLRMQSIANDIEGLDYAEASSRLAAYTLTLQAAQQSY
                     VKIQNLNLFNYL"
     gene            5860..6864
                     /gene="pseB"
                     /locus_tag="PK144_07475"
     CDS             5860..6864
                     /gene="pseB"
                     /locus_tag="PK144_07475"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNF65606.1"
                     /translation="MFDGKSILITGGTGSFGRTFARTLLARHQPERVIVYSRDELKQY
                     EMAQEPAFARERAMRFFIGDVRDRERLRQAMRGVDYVVHAAALKHVPVAEYNPMECIK
                     TNVYGGENVIAAALDNDVAGVIALSTDKAANPANLYGASKLASDKLFIAANNLAGRHR
                     TRFAVVRYGNVVGSRGSVLPLFRRLVAEGARTLPITDARMTRFWITLQQGVDFVLSGF
                     ARMQGGELFVPKLPSARTVDLAVAVAPELPHEIIGIRPGEKLHEVMCPRDDSRLTLEF
                     ADHFVIRPCISFNRPVDYRANPLGETGTEVADGFKYDSGSNPHVLDVPQLRRLIAEVA
                     "
     gene            6870..8054
                     /gene="pseC"
                     /locus_tag="PK144_07480"
     CDS             6870..8054
                     /gene="pseC"
                     /locus_tag="PK144_07480"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HNF65607.1"
                     /translation="MSAVPAPSVSIPYGRQDISEADIAAVVEVLHSDWLTQGPAVPRF
                     EQLLAARTGARHAVAVNSATSALQLVYRALGIGPGSRVWTSPNTFVATASAAALLGAE
                     VDFIDIDPRTLNLCPQRLEERLEAAGRDGTLPDLLVPVHFAGRSCDMAAIGALAKRYG
                     FRVVEDAAHALGAEADGVPVGACRHSDAVVLSFHPVKIVTTGEGGAVLCQDDALATRL
                     QELRSHGITRDPARMPAEPDGAWQYDQLEPGWNFRMTDLQAALGASQLGRLDAFLARR
                     RALAVRYEALLATLALQRPQRDARMRSAWHLYVVQLADAPLRRRVFDALRAAGIGVNV
                     HYRPVYLQPWWRARGFAPGHCPEAERYYAGALSLPLHPRLSESEQNHVVLQLGEALAG
                     LS"
     gene            8126..8884
                     /locus_tag="PK144_07485"
     CDS             8126..8884
                     /locus_tag="PK144_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNF65608.1"
                     /translation="MNRTVAIIQARMGSTRLPGKVLLPLCGHSVLAHVITRALAVPEV
                     DAVVVATSTLAADDAVAAEAARYRAMVCRGPEDDVLERYRLAAAEAAADTVIRITSDC
                     PLLDPAVVGAMLTHFLQQRGAGRALDYLSNGLQRSFPRGLDAEIFTRAVLERCAREAW
                     EPHMREHVTPYVYQHPDRFRIEAWTQPLDQSAQRWTLDTPEDWALISAIFGALGDGLN
                     VFPSTSVHALLDAHPDWRALNAHIEQKPLPAAAA"
     gene            8905..9972
                     /gene="pseG"
                     /locus_tag="PK144_07490"
     CDS             8905..9972
                     /gene="pseG"
                     /locus_tag="PK144_07490"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HNF65609.1"
                     /translation="MSPCLAIRADAGHALGSGHVQRCLALASALRARGLTLRFLMRAC
                     PGHLLDEVRAAGHEVRALPALLPDWLTDATVCLSTLEGCAGLIVDHYGLDARWQRAIT
                     WASGLPVLLAIDDLADRPHAALLLLDVNDFEAGGQRYAELLPPGSRTLLGPQHALLRE
                     EFARERARLRTREAAVRRVLINFGGSDPRDATAIAWQALQTPACAGLELDVIVGSAYV
                     DRSGLAEACAARPHTAFTVQAHDMAARLAAADLAIGAGGTGCWERAALGVPALACAIA
                     DNQRATVAALAARGALRALPEPDVDAWRAAILDLIGDPFARAALGRAAQSLCDGAGAG
                     RVADHLLALLAEGRNAQRQSA"
     gene            9987..10886
                     /locus_tag="PK144_07495"
     CDS             9987..10886
                     /locus_tag="PK144_07495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF65610.1"
                     /translation="MNAQPHPTVTDPELGFRRLDPLPAADELAAFYESRYYDLIRRGG
                     RAPEIGRLLAGGETAAREREWLAATLYADLVDGLETHGAGGGRVLDIGAGTGDLVAHL
                     QSAGLRASGIEPSQDASRLARERGLDVAAATVEQWAADPAHRGAYAGAVLINVLEHVP
                     QPQALLATIGTLLAPGGVLCVRVPNDFSDWQAAAAAACGHDGWWVAAPDHIHYFSHDS
                     LARTLAACGFEPVQLTTDFPMEFFLLMGEDYVADPALGPGLHERRRRFELALPAALRR
                     RLYAAFASVGAGRNTLAFAVRRP"
     gene            10883..>11631
                     /locus_tag="PK144_07500"
     CDS             10883..>11631
                     /locus_tag="PK144_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF65611.1"
                     /translation="MNAPAPAPGCVLITSAARKVWLVEAFRRALAAEGGGEVLAADCD
                     ALAPALYRADRGLLLPRADAPGYEATLLDLCRRERVELLVPTRDAELPWFAERRARFA
                     EIGTRVLVSAPAAIERCRDKRAFTDFCAAHGFATPPAVTLPAAADDFPLFAKPRIGQG
                     SGGARRIESAAGLAGLDAEHWIVQRFDGGPEYTLDVCAGFDGTVLSVVPRRRLQVLAG
                     ESITGVTVMDPALVELGAAVVRALGLIGHATV"
BASE COUNT         1682 a         4254 c         4041 g         1654 t
ORIGIN      
        1 cgcttcgctc ctggtctcgg ggtcgctcat gctttcttcc gcggtgagta cgggggtggg
       61 gggaattatt ctcaggctct cagccggcca gggccgccac catggcaatc ccggcgtcga
      121 tctccagccg ctgccccgcg aagtcccgct gccgcagatc cagcggcacg gcccagtccc
      181 acttgccggc ggcaagattg ccgatgccgt cgagcagggc ctgcggagtg gtttcgccgg
      241 cggcgacgac atcgagcagg gcatcagcac gccagggcag ccgccaggtg atgtgtccgc
      301 cgagcgcgag ccatgggtgc cggtccggtt cgaggccgcg aagcgttggt gggcttcggc
      361 gagccgttcg cgggcgccgt cgtcgagccc gggccgccaa gacacaaggg tgcgcatgcc
      421 tgcgcaggcg ttgcgactgc gcgtgcgttc agccgccccc gccatcccgt agcagccgga
      481 ccatctcggt cccggccgcc cggtcgggca ggcggctcgc cagcgtcgcg atcaggcagg
      541 cgagcgacag cgcgatcgcc atccacaccg gcgcgtgcag tgccttgtag agccccagcc
      601 ccggcacgag ccaggcgagg ccggccagga agcgcagcag cgcatgccac cagccgatgc
      661 gcccgccgcc gggcagctgc acacgcagct tccaggtgcg caggccgagc gtctggccgc
      721 cgtggaccca gaagccgccg tagtacgcga agcagacgag gaacacgaaa ctggtgttcc
      781 acgggttgcc ggcgagcccg gcatgctgcc cgtagccttc ggccccgagc agcagccggc
      841 cgagcccggt ggccagcgcc tcggccatga ccagcacgcc ggcgagcggc agcacgtcgt
      901 agaccgcggc ggcgaggcga cgaaacagcg gggcgggaac gaagcggtcg gcggtgaccg
      961 gcggcagcag cgacatggac aggaccgggg caggaaaacg gcggggcatt taactatgtt
     1021 ccgcgaggcc gccgacagtg cggccgacgg ccgcagcgcc gtcacacgac caagcaccac
     1081 cggggagcct gaatgagcat cgtggccgat ctgatgaacc tgcccggcgt catcgccgcc
     1141 ggcgagtacg cgttccgcgg cgaccgctat tcctatcgcg ggcagctcac cgaggatcag
     1201 gcacgcatgt gcacgatcct ctgccgggcc tcgacgatgg gcatcgtcat gcagggccgc
     1261 atggccgaaa ccgcgctgcc cggcgccggc atccgtccgc gcggctgggc gatgcgcgga
     1321 cccgagcacg cgatgtgcgt ggtcgggaat gtcttctgcg tgatgaagaa ccagtccggc
     1381 acgatggact cggtgctcgc gcgcctgaac acggcgatgg ccgacgtcga catgcacctc
     1441 gtctgatccg cgccggcagc accacacccg tacgcgacct gccttccaga agtcatcctg
     1501 ccgtcatccg gcgccatccc gccggcggca cccgcgacca gaccatcggc aaagaccgac
     1561 ttcgtcagga gagagaaaca acatggccag actcggaaac ctgctcgacc agcccggcgt
     1621 gctcgccgcc ttcgagttcc gcgccagcgg cgaactgctc gaccacgagc tgcgcgccgg
     1681 ttcaccgatc acgaccgaca tcctcgaact gctggccagc atgtgcgcgg ccaacgtcag
     1741 catctgcggc atgcaggcgc gcggctggga gaagctcacc ggcatgagcg gcttctcgaa
     1801 cgtgcgcgag ttcacgctgg tcggcttcga ctggtcggtg gtcgtcagct gcgcgcagcg
     1861 cgaggcccag cgcgctgccg gccaggagac gatcgcgccg taccaggggg tcgtgctgat
     1921 caaccgcgat gcccgctacg aagcggcctg caaggcgctg gaagcctgag gaggccgaca
     1981 tgatcaagcg cctgctcgca gtcgacggcg tgctcgccgt ctgccgcttc cgcgacgacg
     2041 gcggcatggt cgagagttac ggcgtcgggc tggatcagga ccagctcggc aagctgtcgc
     2101 tggtcgcgca cgaataccgg cgcatgctgc agggccacgc cgacttgttc tcgatgttca
     2161 cgaacatgcg cggctggacg ccgccgcgcg gctgggcggt gcgcggcgaa aagctgacgc
     2221 tgtgcggcgt cgccggcgtc gtctgcatcc tcgacaacgc cgaagccgac tacgacgagg
     2281 tcctgaagga catgatcgag atcgcgcact ggtaggcagg ccgggcgtgc ggggagcgcc
     2341 cggcatcctc cgtgcgcgtt cgcccgcatc cgcccgcgcc ggcccggatg cagcaagcgg
     2401 cggtcggagc agacaatttc cggccgccgc gcgcgacggc agagggctgg ctttgataag
     2461 ctgcgccggt ggtttcggcg cgacgattgt tgctgctccg ctacgaaacc ccgcccgttc
     2521 cgtaccggag tctgcgaatg ccgtcgtctt tccatcgctg tctgcgggcg ctgctgctgc
     2581 ccgtcgtgct gctggccccg atttccgtcc ctgccgcccc ggcggcgacc gaaccggtcg
     2641 ccgccgagct gcgtgcccgt ctcgccacgc ccgaggcgct ggccggcgag gcgctgcgtg
     2701 agcgtccgct gctcgcgcgc ttctacgccg cccgcgccgg caagccggcc tggagcgagc
     2761 cgcgcctgct cgatgcgctg ctcgccgccg tcgacaaatc ccccgaggac ggcctcgatg
     2821 ccggcgacta tcaccgtgcc cggctggcga cggcgatcga cgccgcccgc cgcggcaggc
     2881 tcgatgcgac cgggctggcc gcgctcgacg tgctcgcgac cgatgcctgg ctcgcgctcg
     2941 cgcaggacct gctctccggg cggctcgacc cgcagcagct cgacaaggac tggacgctgc
     3001 gcccgcgcag ccgcgatctg gtcgcggcgc tgaacgaggc gctcgcgaac cggcgcatcg
     3061 gcgagtcgct cgcggcgctg gcgccggcct accccgaata cgcgcagctg cgccgcggtc
     3121 tcgccgagct gcgccgccag ctcgcggccg gcggctggcc gacggtcggt gcgctcgcga
     3181 agggcgagaa gatcgaaccc ggccagccgc acccgcgcgt gcgcgagatc cgcgcgcggc
     3241 tgctggcgac gaaggaagtc gaggcgacga cgccggtgct ggcgccggtc agcgtgccgg
     3301 ttccggctcc ggctgcaccc gcagcggcca cgacggcagc cggcggcgag accgccgcca
     3361 cgcctgcgca gccggcggcc gcggagccgg cgccggtcga gaccgtgctc gctgaggtct
     3421 acgacgccac gctggtcgag gccgtgcacc gcttccaggc ccgccacggc ctgaccgagg
     3481 acggcgtgat cggcgcggcg acggtcgcgg cgatgaacgt cagcgtgcgc gagcgcatcg
     3541 cacaggtgcg gctgaacctc gaacgctggc gctggctgcc cggcgatctc ggccagcgct
     3601 acatcgtcgt caacatcccg ggcttcgagc tgcaggtgat cgagcgcggc aggccggtgc
     3661 tcgaagcgaa ggtcgtggtc ggcacgcagg tcaagccgac gccggtgttc accgggcgca
     3721 tgcagcaggt cgtgttcgcg ccgtactgga acgtgccggg cacgatcgcc tccgaggagc
     3781 tgctgccgaa gctgcgcaag aatcccggcg agttcgtgaa gaccaacatg aaggtcatca
     3841 ccggcagcgg tgccgtggtc gatccgaccg ccgtgaactg gggcgagtac caccccggca
     3901 acttcccgtt ccggctgcgt caggagccgg gcgcggccaa tccgctcggg cgcgtgaagt
     3961 tcatcttccc gaaccgctac gacgtctacc tgcacgacac ctcgaagccg gcgctgttcg
     4021 agcgcagcca gcgcacctac agccacggct gcatccgcat ctccaggccc gcggagctgg
     4081 ccgaatacct gctgaagagc gatccgcgct ggaaccgcgc cgcgatcgat gcggcgatgg
     4141 atgccgggcg cgagcgtgcg gtgccggtgc cggagccgct gccggtgcat ctgctgtact
     4201 ggacgagctg ggtcgatgcg cagggcctgc tgcagttccg ggccgatgtc tacggacgcg
     4261 atctggcgct gcgcaaggcg ctgtcggcgc tgtagcgcgc gccggcctgt cgtcgccggc
     4321 aaacggccgg cgacggattt ccgcgccggt gacggcgtca agaatccggc gcaaggccgg
     4381 attctgcttt catgacaatg aattaatgat ctggcgcgaa tcctgctgaa gcacggctca
     4441 ccgtccgtcc ccgcaggttg cgcgccatgt ccagcactcc gttgcgatgc cccgaaccgt
     4501 ccgctccgtg cggccggtgc gcggatcggc gtgtggccgc gcctgccgtc ttctgcatct
     4561 tcggcagtac gaggagcgcg ccatgatccg catttcgacg tcgtacaccc aggaacaggg
     4621 cgtgcagtac atgcgcgatg cgcagaagcg cgtcgtcgac acccaggccg agctggcgac
     4681 cggcaagcgc atctcgtcgc cgtccgatga tccggtcggc gcggcgcgcc tgatcgacct
     4741 gcatcaccag atcgacagca ccgcgcgctt ccagaccaac atcgcgatgg cgaccgggcg
     4801 gctcgcgacc agcgacagca tcctcgatga ggccgggcag ctgacgcagc gcgcgcgcga
     4861 actggtcgtg caggccaaca acgcaccgct cgccgacagc gaccgcgtca gcatcgcgca
     4921 ggagctgcgc cagatccgcg gccagctgct cgaacacgcc aacagcaagg acggtaacgg
     4981 cgactacctg tacgccggct acaggaccca gaccacgccg ttcatcgaga ccaccggcgg
     5041 ccaggtcgtc tatcagggcg acagcggcca gcgcctgctc ggcatcgcgc cgacgcgctc
     5101 ggtggcggtc tccgatgccg gcgacagcgt gttcatgaac atccgcgccg gcaacggcgt
     5161 gttcgcgacc acggccgatg ccggcaacac cggttcggcg gtcatcgaac ccggcgacct
     5221 gctcgacccg accgcgttcg agcaccaccg cttccagatc gatttccagt cctcgaccga
     5281 tttcacgatc accgacctga gcgaggtgcc gccgaccggg ccggggccgg tgcagacgct
     5341 gccggccgac ggtcgcatcg agttcaacgg catctcggtg aagctcaccg gcgcgccggc
     5401 gaacggcgac cgcttcgagg tcgcaccggc gccgtaccag gacgcgttca cgacgctcga
     5461 caaggtcatc accgcgctgg aaaccccggt cgccgccacc ggcgcggcgc gcacgaagtt
     5521 cgcgcagacc atggccgacg cgatgcagaa cctcgaccag atgcattcga acttcctcga
     5581 cacgcgcacg cagatcggcg cccgcctcaa cggcctcgaa acccaggaat ccgcgaacag
     5641 cgaattcagc ctgcgcatgc agtcgatcgc caacgacatc gaagggctgg actacgccga
     5701 ggcgagcagc cggctcgcgg cctacacgct gacgctgcag gccgcgcagc agtcctacgt
     5761 gaagatccag aacctgaacc tgttcaatta tctgtaatca cctccgtcac ccgtcatcct
     5821 ccgaaactga ctgcagtgcg ctgcaagccg aggcacgcca tgttcgatgg caagagcatc
     5881 ctgatcaccg gcggtaccgg ctccttcggc cggaccttcg cgcgcacgct gctggcgcgc
     5941 caccagcccg agcgcgtgat cgtctattcg cgcgatgagc tgaagcagta cgagatggcg
     6001 caggagccgg ccttcgcgcg cgagcgggcg atgcgcttct tcatcggcga cgtgcgcgac
     6061 cgcgagcgcc tgcgtcaggc gatgcgcggc gtcgactacg tcgtgcacgc ggcggcgctc
     6121 aagcacgtgc cggtcgccga gtacaacccg atggagtgca tcaagaccaa tgtctacggc
     6181 ggcgagaacg tcatcgccgc ggccctcgac aacgacgtgg ccggcgtcat cgcgctcagc
     6241 accgacaagg ctgccaaccc tgcgaacctg tacggcgcct cgaagctcgc ctccgacaag
     6301 ctgttcatcg ccgccaacaa cctcgccggg cgccaccgca cgcgcttcgc ggtcgtgcgc
     6361 tacggcaacg tcgtcggctc gcgcggctcg gtgctgccgc tgttccggcg gctggtcgcc
     6421 gaaggcgcac gcacgctgcc gatcaccgat gcgcgcatga cgcgcttctg gatcacgctg
     6481 cagcagggcg tcgacttcgt gctgtcgggc ttcgcacgca tgcagggcgg cgagctgttc
     6541 gtgccgaagc tgccgtcggc gcgcacggtc gatctcgcgg tcgcggtcgc cccggagctg
     6601 ccgcacgaga tcatcggcat ccgccccggc gagaagctgc acgaggtcat gtgtccgcgc
     6661 gacgattcgc gcctgacgct ggagttcgcc gatcacttcg tgatccgtcc gtgcatcagc
     6721 ttcaaccgcc cggtcgacta ccgcgccaat ccgcttggcg agaccggcac cgaggtcgcc
     6781 gacggcttca agtacgactc cggcagcaac ccgcacgtgc tcgacgtgcc ccagctgcgc
     6841 cggctgatcg cggaggtcgc gtgaagcgca tgtccgccgt cccggcgccg tcggtgtcga
     6901 tcccctacgg ccgccaggac atttccgaag ccgacatcgc cgccgtcgtc gaggtgctgc
     6961 actccgactg gctgacgcag gggccggccg tgccgcgctt cgagcagctg ctcgccgccc
     7021 gcaccggggc gcgccacgcg gtcgccgtca acagcgcgac ctcggcgctg cagctcgtct
     7081 accgcgcgct cggcatcggt ccgggctcca gggtctggac cagcccgaac accttcgtcg
     7141 cgaccgcgag tgccgcggcg ctgctcggcg ccgaggtcga tttcatcgac atcgacccgc
     7201 gcacgctgaa cctctgcccg cagcggctgg aggaaaggct cgaagccgcc gggcgcgacg
     7261 gcacgctgcc cgatctgctc gtgccggtgc atttcgccgg ccgctcctgc gacatggccg
     7321 cgatcggcgc gctggcgaag cgctacggct tccgtgtggt cgaggatgcc gcgcacgcgc
     7381 tcggcgccga ggccgacggc gtgccggtcg gcgcctgccg gcattccgat gccgtggtgc
     7441 tgagcttcca cccggtcaag atcgtcacga ccggcgaggg cggggcggtg ctctgtcagg
     7501 acgatgccct tgccacgcgc ctgcaggagc tgcgcagcca cggcatcacc cgcgatccgg
     7561 cgcgcatgcc ggccgagccg gacggtgcct ggcagtacga ccagctcgaa ccgggctgga
     7621 acttccgcat gaccgacctg caggccgcgc tcggcgcgag ccagctcggc cggctcgatg
     7681 ccttcctcgc gcgccgtcgt gcgctcgccg tgcgctacga ggccctgctc gccaccctgg
     7741 cgctgcagcg cccgcagcgc gatgcgcgca tgcgctcggc ctggcacctg tacgtcgtgc
     7801 agctcgccga tgcgccgctg cgccgccggg tgttcgacgc gctgcgcgcc gccggcatcg
     7861 gcgtcaacgt ccattaccgc ccggtctacc tgcagccgtg gtggcgcgcg cgcggcttcg
     7921 cgcccggcca ctgccccgag gccgaacgct attacgccgg cgcgctgagc ctgccgctgc
     7981 atccgcgcct gagcgaatcc gaacagaacc acgtcgtcct gcagctcggg gaagcgctcg
     8041 ccggcctgtc gtgacgccga tccgcagccg aagccaaagc tgaaaccgaa gccggaactt
     8101 gcggaatttc aggagaaccg ccgccatgaa ccgcaccgtc gcgatcatcc aggcccgcat
     8161 gggttcgacc cgcctgcccg gcaaggtgct gctgccgctc tgcggccata gcgtgctcgc
     8221 gcacgtgatc acgcgcgcgc tcgccgtgcc cgaggtcgat gccgtcgtcg tcgcgaccag
     8281 cacgctcgcc gccgacgatg cggtcgccgc cgaggccgcg cgctaccggg ccatggtctg
     8341 ccgcggtccc gaggacgacg tgctcgaacg ctaccggctc gccgccgccg aagcggcggc
     8401 cgacaccgtg atccgcatca cgtcggactg tccgctgctc gatccggccg tggtcggcgc
     8461 gatgctgacg catttcctgc agcagcgcgg cgccggccgg gcgctcgatt acctcagcaa
     8521 cggcttgcag cgcagcttcc cgcgcggcct cgatgccgag atcttcacgc gcgccgtgct
     8581 cgagcgctgc gcgcgcgagg cctgggagcc gcacatgcgc gagcacgtga cgccttacgt
     8641 ctaccagcac ccggaccgct tccgcatcga ggcctggacg cagccgctcg atcagtccgc
     8701 gcagcgctgg accctcgaca cgcccgagga ctgggcgctg atctcggcga tcttcggcgc
     8761 gctcggcgac ggcctgaacg tctttccgag tacctcggtg catgcgctgc tcgacgcgca
     8821 tccggactgg cgcgcgctca atgcccacat cgaacagaag ccgctgccgg cggcagcggc
     8881 ctgacgagcc ggagcgagac catcatgagt ccctgtctgg cgatccgcgc cgatgccggc
     8941 cacgcgctcg gcagcggtca tgtgcagcgc tgcctggccc tggcttcggc gctgcgcgcg
     9001 cgcgggctga cgctgcgctt cctgatgcgc gcctgtcccg gccacctgct cgacgaggtg
     9061 cgtgccgccg gccacgaggt gcgggcgctg ccggcgctgc tgccggactg gctgaccgat
     9121 gccaccgtct gcctgtcgac gctcgaaggc tgcgccggcc tgatcgtcga tcactacggt
     9181 ctcgatgcgc gctggcagcg cgcgatcacc tgggcgagcg gtctgccggt gctgctcgcg
     9241 atcgacgatc tcgccgatcg tccgcacgcc gcgctgctgc tgctcgacgt gaacgacttc
     9301 gaggccggcg ggcagcgcta tgccgaactg ctgccgcccg gcagccggac gctgctcggt
     9361 ccgcagcatg cgctgctgcg cgaggaattc gcccgtgagc gggcgaggct gcgcacccgc
     9421 gaggcggcgg tgcggcgcgt gctgatcaac ttcggcggca gcgatccgcg cgatgccacg
     9481 gccatcgcct ggcaggcgct gcagacgccg gcctgcgccg ggctggaact cgacgtgatc
     9541 gtcggcagcg cctacgtcga tcgcagcgga ctcgccgaag cctgcgcagc gcgaccgcac
     9601 accgcattca ccgtgcaggc gcacgacatg gcggcgcgtc tggcagcggc cgatctggca
     9661 atcggtgccg gcggtaccgg ctgctgggag cgtgccgcgc tcggcgtgcc ggcgctggcc
     9721 tgcgcgatcg ccgacaacca gcgtgccacc gtcgcggcgc tggcggcccg cggtgcgctg
     9781 cgggcgctgc cggaaccgga cgtggacgcc tggcgtgccg cgatcctcga cctgatcggt
     9841 gacccgttcg cgcgcgcggc cctcgggcgc gcggcgcagt cactctgcga cggcgccggt
     9901 gccgggcgcg tcgccgatca tctgcttgcc ctgctggccg aaggtcgcaa cgcccagcgc
     9961 cagtccgcct gaccgaggtt gtcatcgtga atgcccagcc gcacccgacc gtcaccgatc
    10021 ccgaactcgg cttccgccgg ctcgatccgc tgccggccgc cgacgaactc gcggccttct
    10081 acgagagccg ttactacgac ctgatccgcc gcggcggccg ggcgccggag atcggccggc
    10141 tgctcgccgg cggcgagacc gcggcgcgcg agcgcgagtg gctggcggcg acgctgtacg
    10201 ccgacctcgt cgacggactg gaaacacacg gcgccggcgg cggccgggtg ctcgacatcg
    10261 gtgccggcac cggtgacctg gttgcgcacc tgcagtcggc gggcttgcgc gcgagcggca
    10321 tcgagccttc gcaggatgcg agccgcctgg cacgcgagcg cgggctggac gtggccgccg
    10381 cgaccgtcga gcagtgggcc gccgatccgg cacatcgcgg cgcctacgcc ggcgcggtgc
    10441 tgatcaacgt gctcgaacac gtgccgcagc cgcaggcgct gctcgcgacg atcggcacgc
    10501 tgctcgcgcc cggcggcgtg ctctgcgtgc gcgtgccgaa cgacttcagc gactggcagg
    10561 cggcggcggc tgcagcctgc ggccacgacg gctggtgggt cgccgcgccc gatcacatcc
    10621 attacttcag ccacgactcg ctggcacgca cgctcgccgc ctgcggcttc gagcccgtgc
    10681 agctgacgac cgacttcccg atggagttct tcctgctgat gggcgaggac tacgtcgccg
    10741 atccggccct cggccccggg ctgcacgagc ggcgccggcg cttcgagctg gcgctgccgg
    10801 cggctttgcg ccggcgcctg tacgcggcgt tcgcgtcggt cggggccggg cgcaacacgc
    10861 tcgccttcgc ggtgcggcgg ccgtgaacgc gccggcccct gccccgggct gcgtgctgat
    10921 caccagcgcc gcgcgcaagg tctggctggt cgaggccttc cgccgcgcgc tggccgccga
    10981 gggcggcggc gaggtgctgg cggccgactg cgatgcgctg gccccggcgc tgtatcgcgc
    11041 cgatcgcggc ctgctgctgc cgcgggccga tgcgccgggc tacgaggcga cgctgctcga
    11101 tctgtgccgg cgcgagcgcg tcgagctgct ggtgccgacc cgcgatgccg aactgccgtg
    11161 gtttgccgaa cgccgcgcgc gcttcgccga gatcggcacg cgcgtgctgg tgtcggcgcc
    11221 ggcggcgatc gaacgctgcc gcgacaagcg cgcgttcacg gacttctgcg ccgcacacgg
    11281 ttttgcgacg ccgccggcgg tgacgctgcc ggcagccgca gacgatttcc cgctgttcgc
    11341 gaagccgcgc atcggccagg gatcgggggg ggcgcgccgt atcgagtccg cggccggact
    11401 ggccgggctc gatgcggagc actggatcgt gcagcgcttc gacggcgggc ctgaatacac
    11461 gctcgatgtc tgcgccggct tcgacggcac ggtgctgtcg gtcgtgccgc gccggcggct
    11521 gcaggtgctc gccggggagt cgatcaccgg cgtcacggtc atggacccgg cgctggtcga
    11581 actcggcgcg gccgtcgtgc gcgcactcgg cctgatcggc cacgccaccg t
//
LOCUS       DAOQIE010000093        11602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2751_length_11602_cov_7.132502, whole genome shotgun sequence.
ACCESSION   DAOQIE010000093 DAOQIE010000000
VERSION     DAOQIE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2751_length_11602_cov_7.132502"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PK144_07505"
     CDS             complement(<1..928)
                     /locus_tag="PK144_07505"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNF65612.1"
                     /translation="MSEINKVVLAYSGGLDTSVILKWLQETYQCEVVTFTADIGQGEE
                     LEPARAKALAMGVQQIYIDDLREEFVRDFVFPMFRANTLYEGEYLLGTSIARPLIAKR
                     LIEIANETGADAISHGATGKGNDQVRFELGAYALKPGVKVIAPWREWDLTSRETLLDY
                     AEKHGIPVDRYGKKSPYSMDANLLHISYEGQVLENPWAEAEEDMWRWTVAPEAAPDKA
                     TYLELTFRAGDVTAIDGVDMSPATVLATLNRIGGDNGIGRLDLVENRYVGMKSRGCYE
                     TPGGTILLKAHRAIESLCLDREVAHLKDELMPR"
     gene            complement(1063..2061)
                     /gene="corA"
                     /locus_tag="PK144_07510"
     CDS             complement(1063..2061)
                     /gene="corA"
                     /locus_tag="PK144_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNF65613.1"
                     /translation="MTPAKEECAGIVICAAYADGRRIGDIKLDDIPQMLRRDGAFVWL
                     GLYEPDEALMQRVQQCFDLHDLAVEDAHKAHQRPKMEAYGDSLFIVLRTARWDDSAET
                     AAYGETHVFLGHNYLVSVRHGASQTYRPARLASERQPQLLAHGPSFALYALMDFVVDN
                     YFPVLSAYEDRLDELEADIFSGGADRDSTEEIYRLKRELVTLRRASLPLVDVCSDLMS
                     LRVALVPDSIRPYFRDVLDHLKRINDTLDTMREMLATVLQVSLALASVRQNEVTKALA
                     GWAAILAVPTVVFGLYGMNFDKMPELHWHYGYPAIVVATFLVCVWLYLRLKRTGWL"
     gene            2164..3198
                     /gene="pyrC"
                     /locus_tag="PK144_07515"
     CDS             2164..3198
                     /gene="pyrC"
                     /locus_tag="PK144_07515"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF65614.1"
                     /translation="MRLTLTRPDDWHLHVRDGAALATVVPHTARQFARAIIMPNLKPP
                     VTTTGQALAYRERILAAVPAGLDFEPLMTLYLTDRLDPAEIARARASGCIVACKLYPA
                     GATTNSDAGVTDIRLIHPVLAEMEKHGLLLLVHGEVTDADIDLFDREAVFIDRVLAPL
                     LRDFPGLKVVMEHITTREAVDFVAGAGPHLAATLTAHHLLYNRNAIFQGGIRPHYYCL
                     PVLKRETHRQALLAAATSGNPKFFLGTDSAPHARHTKEAACGCAGCYTAPFALELYAE
                     AFESAGALDRLDAFASRFGPAFYGLPVNAGTVTLEKTAVEVPAGYPYPEGETLVPLRA
                     GERLAWRQIA"
     gene            3296..4210
                     /locus_tag="PK144_07520"
     CDS             3296..4210
                     /locus_tag="PK144_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF65615.1"
                     /translation="MKTRSSAASRPPLPARDGVDPCSLRLPAGDWKELLGFLQERFPD
                     DSYVPWTARLARGEVVSEDGETLTTTSTYRPGLRIWYYREPGPEAALPYVETVLHRDA
                     HLLVVDKPHFLPVVPGGPWLRETLLVRLRVRLGLPGLTPLHRLDRLTAGLVLFSVDPA
                     TRAAYAALFPERRITKTYEALAPALPEVPAVRRSRLVAGEPFFRMCEVAGPPNTETRI
                     GLIERRDALGRYRLQPLTGRKHQLRVHMAALGAPVLNDPLYPQLQAGARDDPARPLAL
                     LARELRFTDPLSGEPRAFCSRLHLEFPA"
     gene            4223..4867
                     /locus_tag="PK144_07525"
     CDS             4223..4867
                     /locus_tag="PK144_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNF65616.1"
                     /translation="MTRKLFWDDPYRSALDTVVASVDGETVRLAETIFYAFSGGQESD
                     HGCIGGLPVLEARRQGHDIAYTLPAGHGLQGGMPVTVEIDWPRRYRLMRLHFAAELVL
                     ELVCARCPGIDRIGAHIGADKARIDFAWPQPLTPLLPDLHRQVSALIEADRPIISAFS
                     DAANERRYWEVEGIARVACGGTHLRRTGEVGVLALKRRNVGKGKERVEILLADG"
     gene            complement(5007..5366)
                     /locus_tag="PK144_07530"
     CDS             complement(5007..5366)
                     /locus_tag="PK144_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65617.1"
                     /translation="MYALNAEDLERMGMASLGLAISSQRAALRAGEELSGIGIAASGA
                     YLMIWLGFVGRLLEARSPDTVSECVQRLNEDWQRRVRHDMRLWMATLLETSDRLGSLA
                     AAQPVNPIVPLRRRAAG"
     gene            complement(5521..6042)
                     /gene="phaR"
                     /locus_tag="PK144_07535"
     CDS             complement(5521..6042)
                     /gene="phaR"
                     /locus_tag="PK144_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNF65618.1"
                     /translation="MSEARIIKKYPNRRLYDTAISSYITLEDVKRLVLERADFRVVDA
                     RSSDDITRGILLQIISEQEEQGNPIFTTEVLAHIIRFYGDTLQGMMGSYLEKSLQAFV
                     DQQHLVREQLRNLMGANPLQVISDLAEHNLSIWQKMNERLYKQGVELPRALQPGRAQE
                     TETARTDKPDGKK"
     gene            complement(6268..7449)
                     /locus_tag="PK144_07540"
     CDS             complement(6268..7449)
                     /locus_tag="PK144_07540"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNF65619.1"
                     /translation="MEDVVIVAAARTAIGSFMGTLSTTPAAVLGAKVVQALLARTGLQ
                     PNQIDEVILGHVLTAGCGENPARQVAVNSGLPVEVPAFTINKLCGSGLKAVHLGYQSI
                     VDGDNQIVIVGGQENMSLAPHVLANSRTGARMGDWKLQDTLLLDGLVCAFNNYHMGIT
                     AENIAEKYGISREEQDAFAYASQQKAIAAIDSGRFKDQIVGIQIPQKKGEPLVFDTDE
                     FPNRKSTPEVLAKLKPAFKKDGTITAGNASGVNDGAAALVLMTASKAAELGLKPLARI
                     AGYAKSGVDHKIMGTGPIPAVRKLMQKTGWTLDQLDLIEANEAFAAQAISVNRDLGWD
                     LSKVNVNGGAIAIGHPIGCSGARILTDLVHEMGRRDAKKGVATLCIGGGMGVALAIER
                     D"
     gene            complement(7889..8320)
                     /locus_tag="PK144_07545"
     CDS             complement(7889..8320)
                     /locus_tag="PK144_07545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF65620.1"
                     /translation="MQRSEFRHFIELPVHWGDQDALGHVNNVQFFRYLESARVAYVET
                     VFGLPVRADGEGVILADLQCAFRSQLEYPVTVEVGTRIARLGRSSFDVHAAIWRQGAE
                     APAATSRGVIVWFDFRAQKPAPLPDSLRERVRAWETLRPQE"
     gene            complement(8411..8815)
                     /gene="msrB"
                     /locus_tag="PK144_07550"
     CDS             complement(8411..8815)
                     /gene="msrB"
                     /locus_tag="PK144_07550"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNF65621.1"
                     /translation="MKFRVVKSEAEWRAQLSPEQFAVCRQKATERPFTGRYNEHSAAG
                     TYRCVCCGQPLFASRSKFNAGCGWPSFSLPLDEAAIAEETDRSHGMVRTEVMCSNCGA
                     HLGHVFDDGPGPDGLRYCINSVSLDFEAAGKG"
     gene            8986..9525
                     /locus_tag="PK144_07555"
     CDS             8986..9525
                     /locus_tag="PK144_07555"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNF65622.1"
                     /translation="MTELLAARVLAVPDHPQPGIVFRDVTPLLADAGAFRAAIDGLAA
                     AVRAAGWSPQYLLCPEARGFLFGAPLALALGAGFVPVRKPGKLPRATARADYALEYGT
                     DALEVHRDAFVAGSRVLLVDDVLATGGTLAAVHALAGQLGAEVLGALCLIELPALGGR
                     ARLAPLPVQALLRCGPAPD"
     gene            complement(9437..10075)
                     /locus_tag="PK144_07560"
     CDS             complement(9437..10075)
                     /locus_tag="PK144_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF65623.1"
                     /translation="MARLILFNKPYDVLSQFTDREHGRTTLADFIDLPGVHVAGRLDR
                     DSEGLLLLTDDGRLQARIADPRHKLAKTYLVQVEGEPQEAALEALRRGVVLRDGPTLP
                     AGVRRVPAPDWLWPRTPPIRTRATIPTAWLEIVLREGRNRQVRRMTAAVGLPTLRLLR
                     SAIGDWTLAGLAPGAWRAETVHLPSPAPARSAAAPAPAAAPGGRGRRGPAAR"
     gene            10225..10674
                     /locus_tag="PK144_07565"
     CDS             10225..10674
                     /locus_tag="PK144_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNF65624.1"
                     /translation="MPLTPSRCARCWLAVLLALAWPAMAGVYRWRDADGRMHYGDQPP
                     AGVQAEALPVDPAPPPRSAADAERRLLEDAERRAADTARGRSEARRREARAEAERERR
                     CSRLDERRRQLETGFAERRARGYTMAQERSYRSRLDALERELKSGCR"
     gene            10725..11510
                     /locus_tag="PK144_07570"
     CDS             10725..11510
                     /locus_tag="PK144_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HNF65625.1"
                     /translation="MPRFAANLSFLFTELPFPERFAAAATAGFGGVEFLFPYAWPVEA
                     IAGWRRAASVDSVLFNLPPGDWEAGERGLACLPGREAEFLDGIERALPYALALGTPRL
                     HAMAGLLPTGADPAHCRQVYVDNLRRAAARLAAHGITLLIEPINPRDMPGYFLNTQAD
                     AHAIREAVGAPNLRVQTDLYHAQIVEGDLAVKLERWLAHIGHLQIAGVPGRHEPDHGE
                     IHYPYLFALLDRLGYDGWIGCEYRPAAGTVEGLGWYAPWRTQR"
BASE COUNT         1679 a         4123 c         3979 g         1821 t
ORIGIN      
        1 agcgcggcat cagctcgtcc ttcaggtgcg cgacctcgcg atcgaggcac agcgattcga
       61 tcgcacggtg cgccttcagc aggatcgtgc cgccgggggt ttcgtagcag ccgcgcgact
      121 tcatgccgac gtagcggttc tcgaccagat ccagccggcc gatgccgttg tcgccgccga
      181 tgcggttcag cgtcgcgagc acggtcgccg ggctcatatc gacgccgtcg atcgcggtca
      241 cgtcaccggc gcggaaggtg agttcgagat aggtggcctt gtccggcgcg gcctcgggcg
      301 cgaccgtcca gcgccacatg tcctcctcgg cctcggccca ggggttttcg agcacctggc
      361 cttcgtagga gatgtgcagc aaattggcgt ccatcgagta cggcgacttc ttgccgtagc
      421 gatcgaccgg gatgccgtgc ttttcggcgt agtcgagcag ggtctcgcgc gaggtcaggt
      481 cccattcgcg ccacggcgcg atgaccttga cgccgggctt cagcgcgtag gcgccaagct
      541 cgaagcgcac ctggtcgttg cccttgccgg tcgcgccgtg cgagatcgca tcggcgccgg
      601 tctcgttggc gatctcgatc aggcgcttgg cgatcagcgg ccgggcgatc gaggtgccga
      661 gcaggtactc gccttcatag agggtgttgg cgcggaacat cgggaacacg aagtcgcgca
      721 cgaactcctc gcgcagatcg tcgatgtaga tctgctgcac gcccatcgcc agcgccttgg
      781 cgcgtgccgg ttcgagctcc tcgccctgac cgatgtcggc ggtgaaggtc accacctcgc
      841 actgataggt ctcctgcagc cacttcagga tcaccgaagt gtcgagaccg ccggaatagg
      901 ccagcacgac cttgttgatt tcggacatga acttccccag cgcgcgtcgg cggcggatac
      961 ggatggaacg gtcggccctg cacgccagtc gcgatgccgg ccgtggaaac gcgccagctt
     1021 acgcgccggt cacggccggt caaggcagcc ggcaagcccg cttcacagcc agccggtgcg
     1081 cttgagccgc aggtacagcc acacgcacac cagaaaggtc gccacgacga tcgccggata
     1141 gccgtagtgc cagtgcagct ccggcatctt gtcgaagttc atgccgtaca ggccgaacac
     1201 cacggtcggc accgccagga tcgccgccca gccggccagc gccttcgtga cctcgttctg
     1261 gcgcaccgag gcgagcgcga ggctcacctg cagcacggtc gcgagcattt cgcgcatcgt
     1321 gtcgagcgtg tcgttgatgc gcttgaggtg atcgagcacg tcgcggaagt acgggcggat
     1381 cgagtccggc accagcgcca cacgcagcga catcaggtcc gaacagacat cgaccagcgg
     1441 cagcgacgcg cgccgcaagg tcacgagttc gcgcttgagg cggtagatct cctcggtcga
     1501 gtcgcggtcc gccccgcccg agaagatgtc ggcttccagc tcgtcgagcc ggtcctcgta
     1561 ggccgagagc accgggaagt agttgtcgac gacgaaatcc atcagtgcgt acagcgcgaa
     1621 gctcggtccg tgggcgagca gctgcggctg gcgttcgctc gccagccgcg ccggccggta
     1681 ggtctgcgag gcgccgtggc gcaccgagac cagatagttg tggccgagga acacgtgcgt
     1741 ctcgccatag gcggcagtct cggccgagtc atcccagcgc gcggtgcgca gcacgatgaa
     1801 cagcgaatcc ccgtacgcct ccatcttcgg acgctggtgg gccttgtgcg catcctcgac
     1861 cgccagatca tgcaggtcga agcactgctg cacgcgctgc atcagggctt cgtccggctc
     1921 gtagaggccg agccagacga aggcaccgtc gcggcgcagc atctgcggga tgtcgtcgag
     1981 cttgatgtcg ccgatgcggc ggccatcggc ataggccgcg cagatgacga tgccggcaca
     2041 ttcctctttc gctggtgtca tgttcgtccc cccgtccgcc ggccgggcgt cggcgcccgt
     2101 gccttgagtg ctcgttatac tgccctgctg ctgatcgttc cgttccccgc cggagtgctg
     2161 ccgatgcgcc tgaccctgac ccgccccgat gactggcatc tgcacgtgcg cgacggcgcc
     2221 gcactcgcga ccgtcgtgcc gcacacggca cgccagttcg cgcgggcgat catcatgccg
     2281 aacctgaagc cgccggtgac gacgaccgga caggcgctcg cctaccgcga gcgcatcctc
     2341 gccgcggtgc cggccggact cgatttcgag ccgctgatga cgctgtacct gaccgatcgc
     2401 ctcgatccgg ccgagatcgc cagggcgcgc gcctcgggtt gcatcgtcgc ctgcaagctg
     2461 tatccggccg gcgcgacgac gaattccgat gccggcgtca ccgacatccg gctgatccac
     2521 ccggtgctcg ccgagatgga aaagcacggc ctgctgctgc tcgtgcacgg cgaggtcacc
     2581 gatgccgaca tcgacctgtt cgatcgcgag gcggtgttca tcgaccgcgt gctggcgccg
     2641 ctgctgcggg acttcccggg cctgaaggtg gtcatggaac acatcacgac ccgcgaggcc
     2701 gtcgacttcg tcgccggcgc cggtccgcac ctggccgcga ccctcaccgc gcaccacctg
     2761 ctctacaacc gcaacgccat tttccagggc ggcatccgcc cgcattacta ctgcctgccg
     2821 gtgctcaagc gcgaaaccca ccgccaggcc ctgctcgccg ccgcgacttc cggcaacccg
     2881 aagttcttcc tcggcaccga cagcgccccg cacgcccgcc acaccaagga agccgcctgc
     2941 ggctgcgccg gctgctatac ggcgccgttc gcgctggaac tctatgcgga agccttcgag
     3001 tccgccggcg cgctcgatcg gctcgatgcg ttcgcgagcc gcttcggacc ggcgttctac
     3061 ggactgccgg tcaatgccgg gacggtgacg ctggagaaga cagccgtgga ggtgccggcg
     3121 ggctacccgt atccggaagg cgagacgctg gtgccgctgc gagcggggga aagactcgcg
     3181 tggcgtcaga tcgcctgaat cctgcgcccc gccccggccg tccggatcgt cggtaccggt
     3241 ggccggcccc cggctccccg gatgccgctg cccgctcccg tgaacgctgg catccatgaa
     3301 aacccgctca tccgccgcgt cgcggccacc gctgcctgcg cgcgatggcg tcgatccctg
     3361 ctcgctgcgc ctgccggccg gcgactggaa ggagctgctc ggcttcctgc aggaacgctt
     3421 tcccgatgac agctacgtgc cctggacagc acggctggcg cgcggcgagg tcgtcagcga
     3481 ggacggcgaa accctgacga cgaccagcac ctaccgcccg gggctgcgca tctggtacta
     3541 ccgcgagccg gggccggaag cagcgctgcc gtacgtcgag accgtgctgc accgggatgc
     3601 gcacctgctg gtcgtcgaca agccgcactt cctgccggtc gtgcccggcg ggccgtggct
     3661 gcgcgagacg ctgctggtgc gcctgcgcgt gcggctcggg ctgccggggc tgacgccgct
     3721 gcaccggctc gaccggctga ccgccgggct ggtgctgttc tcggtcgatc cggccacgcg
     3781 cgcggcctat gcggcgctgt ttcccgaacg gcgcatcacc aagacctacg aggcgctggc
     3841 cccggccttg ccggaagtgc ccgctgtgcg ccgcagccgc ctggtcgccg gcgagccgtt
     3901 tttccggatg tgcgaggtcg ccggtccgcc gaacaccgag acgcgcatcg ggctgatcga
     3961 gcgacgtgat gccctgggcc gctaccggct gcagccgctc accggccgca agcaccagct
     4021 gcgcgtgcac atggccgcgc tcggcgcgcc ggtcctgaac gatccgctct acccgcagct
     4081 gcaggccggt gcgcgcgatg atccggcccg gccgctggcg ctgctggcgc gcgagctgcg
     4141 cttcaccgat ccgctgagcg gcgagccacg ggcgttctgc agccgcctgc atctggaatt
     4201 tcccgcctga gagaccaccg ccatgacccg caagctgttc tgggacgacc cgtaccgcag
     4261 cgcgctcgat accgtggtcg cgagcgtcga cggcgaaacc gtgaggctcg ccgaaaccat
     4321 cttctatgcg ttctcgggcg gacaggagag cgaccacggc tgcatcggcg gcctgccggt
     4381 gctcgaagcc cgcaggcagg ggcacgacat cgcctacacg ctgccggccg gacatggcct
     4441 gcagggcggc atgccggtca cggtcgagat cgactggccg cggcgctacc ggctgatgcg
     4501 gctgcacttc gcggccgaac tggtgctgga actcgtctgc gcgcgctgcc cgggcatcga
     4561 ccggatcggc gcgcacatcg gcgcggacaa ggcacgcatc gacttcgcgt ggccgcagcc
     4621 gctgacgccg ctgctgccgg acctccacag gcaggtcagc gcgctgatcg aagccgaccg
     4681 gccgatcatc agcgccttca gcgatgccgc gaacgagcgg cgctactggg aagtcgaggg
     4741 gatcgcacgc gtagcctgcg gcggcacgca cctgcgccgc accggcgagg tcggcgtcct
     4801 ggcactgaag cggcgcaacg tcggcaaggg caaggagcgg gtcgagatcc tgctggccga
     4861 tggctgatct cgccggaacg cgcggactgc cgcatcaagg gccggcctcc gcaaacgacg
     4921 acgcaccgca tgcgccaggg cagtacggtg cgtcgcgagg ccgcggcggg agggagcgga
     4981 agggtccgtc ccggagggct gcggtttcag ccggccgcgc ggcggcgcag cggcacgatc
     5041 gggttcaccg gctgggcggc ggccagcgag ccgaggcggt cgctggtctc cagcagcgtc
     5101 gccatccaca ggcgcatgtc gtggcgcacg cggcgctgcc agtcctcgtt caggcgctgc
     5161 acgcattcgc tgaccgtgtc ggggctgcgg gcttcgagca ggcgcccgac gaagccgagc
     5221 cagatcatca ggtaggcgcc gctggccgcg atgccgatgc ccgacagctc ctcgcctgcg
     5281 cgcagcgccg cccgctgcga gctgatggcc agaccgagac tggccatgcc cattcgctcc
     5341 agatcctcgg cgttcagcgc atacatgacg gctacctctt gatggtgcgt cgcaacattt
     5401 gttgcagtgc aatatagggt tttccctgat gagaagtcaa cgggctccag acatgcaaaa
     5461 gccgcccgaa ggcggccctg acagtccgaa acagaccgga actccggaat ccctgcctgc
     5521 ctacttcttg ccgtccggct tgtcggtgcg cgcggtttcc gtttcctgtg cacgccccgg
     5581 ctgcagtgcc cgcggcagct cgacgccctg cttgtacagg cgttcgttca tcttctgcca
     5641 gatcgacaga ttgtgctcgg cgaggtccga gatcacctgc agcgggttgg cgcccatcag
     5701 gttgcgcagc tgctcgcgca ccagatgctg ctggtcgacg aaagcctgca ggctcttttc
     5761 gaggtagctg cccatcatcc cctgcagcgt gtcgccgtag aaacggatga tgtgtgcgag
     5821 cacctcggtc gtgaagatcg ggtttccctg ctcctcctgc tcgctgatga tctgcagcag
     5881 gatgccgcgc gtgatgtcat cgctggagcg ggcatcgacg acgcggaagt ccgcccgttc
     5941 cagcacgagc cgcttgacgt cttcgagggt gatgtagctg ctgatcgccg tgtcgtagag
     6001 ccggcgattc ggatacttct tgatgatgcg cgcttcgctc atcggttccg accggttggg
     6061 ggacgccagg gccatccgct cgcccttgcg ccgggtcctg caggggaccg cagtgggaat
     6121 gaggaccacg gtctgacgcc gcagtccggt tgggtccggc atttcctccc gggcagtcgc
     6181 ctgccgtcat gccgccgtgc agtcccgccg tcgggaccgc gaaacgtccg gggcggcgtg
     6241 cctcgcgaca cgccgccccg accgggatca atcgcgctcg atcgcgagcg ccacacccat
     6301 gccgccgccg atgcagagcg tcgcgacgcc cttcttggcg tcacggcgac ccatctcgtg
     6361 gacgaggtcg gtcaggatgc gggcgccgga gcagccgatc gggtggccga tggcgatcgc
     6421 gccgccgttg acgttgacct tggacagatc ccagcccagg tcacggttga ccgagatcgc
     6481 ctgcgcggcg aaggcctcgt tggcctcgat caggtcgagc tggtcgagcg tccagccggt
     6541 cttctgcatc agcttgcgca cggccgggat cgggccggtg cccatgatct tgtgatcgac
     6601 acccgacttc gcgtagccgg cgatgcgcgc cagcggcttc aggcccagct cggccgcctt
     6661 gctcgcggtc atcagcacca gcgccgcggc gccgtcgttg acgcccgagg cgttgccggc
     6721 ggtgatcgtg ccgtccttct tgaacgccgg cttcagcttg gcgagcactt cgggcgtgga
     6781 cttgcggttc gggaactcgt cggtgtcgaa caccagcggc tcacccttct tctgcgggat
     6841 ctggatgccg acgatctgat ccttgaaccg gccgctgtcg atcgcggcga tcgccttctg
     6901 ctgcgaggca taggcgaagg catcctgctc ctcgcgcgag atgccgtact tctcggcgat
     6961 gttctcggcg gtgatgccca tgtggtagtt gttgaacgcg cagaccagac cgtcgagcag
     7021 cagggtgtcc tgcagcttcc agtcgcccat gcgcgcaccg gtgcgcgagt tcgccagcac
     7081 gtgcggcgcc aggctcatgt tctcctggcc gccgacgatg acgatctggt tgtcgccgtc
     7141 gacgatcgac tggtaaccga gatgcacggc cttcaggccg gaaccgcaca gcttgttgat
     7201 cgtgaaggcc gggacctcga ccggcaggcc cgagttgacg gcgacctgac gcgcggggtt
     7261 ctcaccgcag ccggccgtca gcacgtgacc gagaatgact tcgtcgatct ggttcggctg
     7321 caggccggtg cgggcgagca gcgcctgcac gaccttggca ccgaggaccg ccgccggcgt
     7381 ggtggacagc gtgcccatga acgaaccgat cgcggtacgg gcggcggcaa cgattacgac
     7441 gtcttccatt ttcaacctcc gataggagct cttgctgact ttcacggatc gggcgatccg
     7501 ccgatttccg cgtagtcatg atttgggacc ggtccggaat gctcgttctc cccctccggc
     7561 acttgcagcg ccggaaattt ccggacaggg cgcctgtttg acgagcgcgg ggcgatactg
     7621 cgggccgggt caaagcttgt caactttgcc cggcagtgca gcagcgtggt ctgcaaggtg
     7681 cagcaaacgc aggccggacg gcagcttggc gaactgcctc cccgtcgccg ccgcacagtg
     7741 taccgcgacg tatcgcggcg cagcacgagc ggttcaaacg ggcggatgac gcaaatcatt
     7801 ccgcctgccg atcaccggca tggtcgatgc ttcaaagagt gccggaagcg gcaccggcag
     7861 aggctgccgg ccggtgccgg ccaacgtcct attcctgcgg tctcagggtt tcccaggcgc
     7921 gcacgcgctc gcgcagggaa tccggcagcg gcgccggttt ctgcgcgcgg aaatcgaacc
     7981 agacgatgac gccgcgcgac gtggcggccg gggcctccgc cccctggcgc cagatcgccg
     8041 catgcacgtc gaagctgctg cgcccgagcc gggcgatgcg ggtgccgacc tcgaccgtga
     8101 ccgggtattc gagctggctg cggaacgcgc actgcaggtc ggcgaggatc acgccctcgc
     8161 catcggcgcg caccggcagt ccgaacacgg tttcgacgta ggcgacccgg gcactttcga
     8221 gatagcggaa gaactgcacg ttgttgacgt ggccgagggc gtcctgatcg ccccagtgca
     8281 ccggcagttc gatgaaatga cggaattcgc tgcgctgcat gcgtgggctc cggcggcggt
     8341 gacggggacg caccaccggt cccggcccgg tggcgcacgg caggcgccag cgcctgccgt
     8401 gcgcgcagca tcagcctttt ccggcggcct cgaaatccag cgacaccgaa ttgatgcaat
     8461 accgcaatcc gtccggtccg ggaccgtcgt cgaacacatg gccgaggtgc gcgccgcagt
     8521 tcgagcacat gacctcggtg cgcaccatgc cgtggctgcg gtcggtctcc tcggcgatcg
     8581 ccgcctcgtc gagcggcagc gagaagctcg gccagccgca gccggcgttg aacttgctgc
     8641 ggctcgcgaa cagcggctgg ccgcagcaga cgcagcggta ggtgccggcg gccgaatgct
     8701 cgttgtagcg accggtgaac ggccgctcgg tcgccttctg gcggcagacg gcgaactgct
     8761 cgggcgacag ctgcgcccgc cactcggctt cggacttgac gacgcggaat ttcatggaga
     8821 cctcctgctg gactgccgtg acgaccggtg cggaccggcg gggaacgacg gccgccggtg
     8881 ccgccggcga ccgggccgat atggcggtgg cgcgggccga ttaccagccg cccgcgcgcg
     8941 acgggctgcc tacactggcc gaagaccgct gccggagacc cggggatgac cgaactgctt
     9001 gccgcccgcg tgctcgccgt gcccgatcac ccgcagcccg gcatcgtgtt ccgcgacgtg
     9061 acgccgctgc tcgccgatgc cggtgcgttt cgcgccgcga tcgacggcct cgccgccgcc
     9121 gtgcgcgccg ccggctggag tccgcagtat ctgctctgcc ccgaggcgcg cggtttcctg
     9181 ttcggcgcgc cgcttgcgct cgcgctcggc gccggcttcg tgccggtgcg caagcccggc
     9241 aagctgccgc gcgccaccgc ccgtgccgat tacgcgctcg aatacggcac ggatgcgctc
     9301 gaagtgcacc gcgatgcctt cgtcgccggc agccgcgtgc tgctggtcga tgacgtgctc
     9361 gccaccggcg gcacgctcgc cgccgtgcac gcgctggccg gacagctcgg cgccgaggtg
     9421 ctcggggcgc tctgcctcat cgagctgccg gccctcggcg gccgcgcccg cctggcgccg
     9481 ctgccggtgc aggcgctgct gcgctgcggg ccggcgccgg actaggcaga tgcacggtct
     9541 cggcccgcca cgcgcccggc gcgagtccgg cgagcgtcca gtcgccgatc gcgctgcgca
     9601 gcagccgcag cgtcggcagg ccgacggcgg cggtcatgcg ccgcacctga cggttgcggc
     9661 cctcgcgcag cacgatctcc agccacgcgg tcgggatcgt cgcgcgcgtg cggatcggcg
     9721 gcgtgcgcgg ccagagccag tccggtgccg gcacgcgccg cacgccggcc ggcagcgtcg
     9781 gcccgtccct gagcacgacg ccgcggcgca gggcttcgag cgcggcctcc tgcggctcgc
     9841 cttcgacctg caccagatag gtcttcgcca gcttgtggcg cggatcggcg atgcgcgcct
     9901 gcagccggcc gtcgtcggtc agcagcagca ggccctcgct gtcgcgatcg agccggccgg
     9961 cgacgtggac gccgggcaga tcgatgaaat ccgcgagcgt cgtgcgcccg tgctcgcggt
    10021 cggtgaactg cgagagcacg tcatagggct tgttgaacag gatcagtctc gccatcgtcc
    10081 gccgccgggt tcgtcgtatc cgccgcaacc gccctggcgc aggcctgtgg gtcttcgtgc
    10141 agccgggcgg tcacgccgtg cagcataccg gcccgctgac gcaagacccc gtgcgaccgg
    10201 cccgggcagg acgggaatgt cccgatgccg ctgacgccat cccgctgcgc ccgctgctgg
    10261 ctggcggtgc tgctcgccct cgcctggccg gccatggccg gggtctaccg ctggcgcgat
    10321 gccgacggcc gcatgcacta cggcgaccag ccgccggccg gcgtgcaggc cgaagccctg
    10381 ccggtcgatc ccgccccgcc gccgcgcagt gcggccgatg ccgagcgccg gctgctggag
    10441 gacgccgagc gacgtgcagc cgacacggcg cgcgggcgca gcgaggcccg ccgccgcgaa
    10501 gcgcgcgccg aggccgagcg cgagcgccgc tgcagccggc tcgacgaacg ccgccgccag
    10561 ctcgaaaccg gattcgccga gcgccgcgcg cgcggctaca cgatggcgca ggagcgcagt
    10621 taccggagcc ggctcgatgc gctggaacgc gagctcaaat ccggctgtcg ctgacaccgg
    10681 cccctgcccc ccgttcttcc tgccatgccg cctgcggagt cgccatgccg cgctttgccg
    10741 cgaacctgag cttcctgttc accgagctgc cgtttccgga acgcttcgcc gccgcggcca
    10801 ccgccggttt cggcggggtc gagttcctgt ttccgtatgc ctggcccgtc gaggccatcg
    10861 ccggctggcg gcgcgccgcc agcgtcgaca gcgtgctgtt caacctgccg cccggcgact
    10921 gggaggccgg cgaacgcggc ctcgcctgtc tgccggggcg cgaggccgag ttcctggatg
    10981 ggatcgagcg cgcgctgccg tatgcgctcg ccctcggcac gccccggctg cacgcgatgg
    11041 ccgggctgct gccaaccggg gccgatccgg cgcactgccg gcaggtctat gtcgacaacc
    11101 tgcgccgcgc cgccgcacgg ctggccgcac acggcatcac gctgctgatc gaaccgatca
    11161 atccgcgcga catgcccggc tacttcctga acacgcaggc cgacgcgcac gcgatccgcg
    11221 aggcggtcgg cgcgccgaac ctgcgggtgc agacggacct gtaccacgcg cagatcgtcg
    11281 agggcgatct ggccgtgaag ctcgaacgct ggctggcgca catcggccac ctgcagatcg
    11341 ccggcgtgcc cgggcgccac gagcccgacc acggcgagat ccactacccc tacctgttcg
    11401 cgctgctcga ccggctcggc tacgacggct ggatcggctg cgaataccgt ccggcggccg
    11461 gcaccgtcga ggggctcggc tggtacgcgc cgtggcgaac gcagcgctga atccgccgcc
    11521 gggcgccgcc gcgccgcttg ccccgcacgc gcccgggccg gccggctatc gccgctggca
    11581 gcgcttgctg gccgcagcgg tg
//
LOCUS       DAOQIE010000094        11468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2798_length_11468_cov_7.730395, whole genome shotgun sequence.
ACCESSION   DAOQIE010000094 DAOQIE010000000
VERSION     DAOQIE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_11468_cov_7.730395"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..3376)
                     /locus_tag="PK144_07575"
     CDS             complement(2..3376)
                     /locus_tag="PK144_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF65626.1"
                     /translation="MIDTSLSGPGTRAHPSRDLLRHWFDPLTLVRGEALLRTGGVLEL
                     RPGTDGSVHGVVRGSHRPRYETRVRLIQNTRGLQLETECSCPVGVDCKHAAALLLRLL
                     ALGSGSDRPACVPPLAPVTPAPASGPSPALSAWLERVGHAALPKPAGDAAERLLYLLQ
                     PQGDAPDACALRVVSARALKKGGYGKPRNLDPGSVLVPGMYPGRFEDADRALLHELAL
                     ARDPYARTLGLAGERGAALLARVLASGRCHWLDENGPLLAEGPARPATVQWQTDPGGG
                     TRLAFVAGPPADAVLPLSPPWYVDAQAGCCGPLSTTLPPALAGALASAPVLEAEDIDP
                     LNDWLERHLPGVALPRPPRLQVATEADFRPQPRLRLYSRELGAAPAAGIGRGTRSGTG
                     LVDGVHLDFGYGDAHWLDWHDDTPVISRLRADRVVERIARRRDLERAAIERLYDADLI
                     ALRNLSWAAERLPRSCRHDFGFHDPDRWADFVLGELPRLRAEGWRIEIEPGFRYDLAE
                     PGEWFAELDESGGEDWFGLALGVELDGERVNLLPCLVELLARLPGGDARSWLAQLGDE
                     RRVMVPLPDGRLLPVPAARIRKILAVLVELYDGEGLNLRGKLSLARSQATRLAELEAA
                     LGDRTLTWHGGERLRTLLTELRRGPQPVAVPEGFHAQLRPYQQQGLEWLQFLRVTQLA
                     GVLADDMGLGKTVQTLAHIACEHAAGRLDRPVLIVAPTSLLPNWRDEAARFAPRLRVL
                     ALRGPDRARDFARIPEHDLVLTTYALLPRDREALARHTYHLLILDEAQAIKNPTAQAG
                     IVARALRARHRLCLTGTPMENHLGELWSLFDFLLPGLLGDARQFQRSFRKPIEKDGDR
                     ERRERLAARVAPFLLRRTKEQVAADLPPKTEIVRTIEIAGPQRDLYEAIRLSMQERVR
                     AEIAAKGLARSHIVILDALLKLRQVCCDPRLVKLGSARAVKGSAKLELLTEMLPELVD
                     EGRRVLVFSQFTQMLALIEKELTRLGIAYVLLTGTTEDRATPVRRFQAGEVPVFLISL
                     KAGGTGLNLTAADTVIHYDPWWNPAAEDQASDRAHRIGQEKPVFVYKLYTEGTVEEKI
                     RDLQVRKRALVESILTERDSAPQLAEDDLDALFAPL"
     gene            complement(3373..3681)
                     /locus_tag="PK144_07580"
     CDS             complement(3373..3681)
                     /locus_tag="PK144_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNF65627.1"
                     /translation="MSYVLAAVALMALATFATRAAPFLLFAGAGERPWLRFLARYLPP
                     AVMTVLVLYCLRDTFTSGAPGPLPALAGVVVTVAVHLARRNALLSIAAGTALHMLLLR
                     "
     gene            complement(3678..4376)
                     /locus_tag="PK144_07585"
     CDS             complement(3678..4376)
                     /locus_tag="PK144_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNF65628.1"
                     /translation="MPSPDVLAAALRTCLPVAFGYVPLGMAFGVLLVQSGQPAWLAPL
                     MGLCIYAGAAQFLAVGLLAARVPLADIALATLLLNLRHAFYGLSMIDRLPVRGWRRWY
                     AVFALTDETWSLLTGTRPPPAFATPPAAHDFVFAVSALNQGWWLLGCTLGGLGGLALG
                     GLDTHGLEFTLTALFVVLLVEQWHAVREPWPFLLAASAGAVSFAFAPPAQFLLVALAL
                     TLAGLLAVQRARPA"
     gene            4917..5357
                     /locus_tag="PK144_07590"
     CDS             4917..5357
                     /locus_tag="PK144_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65629.1"
                     /translation="MNNKLMACLLAATLALPLSAPAQAGSGDALIGGGVGGALGAVVG
                     HEISGRDGAIVGGALGAALGAAATTQGDRDYRYRRVYNGPVYERPYVVERPYYAPPPP
                     RYYAPPPRYVVERRVYVEDRGYRRGPPPWARHHHRHHRHWRDWD"
     gene            5543..7183
                     /locus_tag="PK144_07595"
     CDS             5543..7183
                     /locus_tag="PK144_07595"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNF65630.1"
                     /translation="MSAVPRLRAAGLVLIGLTATLVSACGGGGGPYAGAPVAGLPPPK
                     VTGALAGGMSGALLAKATEPRGAPAALPAAQFPQAGKSPVVRTAEQPVSTFSLDVDTA
                     SYALVRRLLNDGRLPPVDAVRIEEMVNYFPYAWPAPEARSRPLQVSTALLPTPWNPDT
                     RLLRIGIRGWAPATHERPPANLVFLIDVSGSMDAPDRLGLLQKSLGLLVERLRPEDRV
                     AIVSYAGGVRTVLEPTSGRERTKILAALDGLAAGGSTAGAAGLEQAYRLAREHFDRQA
                     VNRVILGTDGDFNVGLSDPAGLAQYVSEQRKAGVYLSVLTVGLDNLHDGTAQALAQAG
                     NGNAAYLDSVQEGRRVLVEQLAATMIPVADDAKVQVEFNPAEVSEYRLLGYETRALAR
                     ADFTNDAVDAGDLGVGHTVTALYEITPAGARARIEPGRYEAPAATAPNRHPGELAWVK
                     LRWKQPGAARSEALAQPVRAADAFAGIAAADADTRFAVAVAAFGQWLRQDTELRGYGI
                     DAIETLAQGARGEDGEGRRAEFVQLVRQAAALRGAPAR"
     gene            7246..8124
                     /locus_tag="PK144_07600"
     CDS             7246..8124
                     /locus_tag="PK144_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNF65631.1"
                     /translation="MTFSIVAHDPETGDLGVAVQSKFPNVGVSIPYAQAGVGAIATQC
                     YCNTSFGPRGLALLGHGASPQQAVEILTGNDAEREFRQLGIVDVQGRAASFTGARCFD
                     WAGSRHAAHVAVQGNVLAGAGVLDAMLERYQAGAGEPLPLRLIAALAAGQAAGGDRRG
                     QQSAAVKVVRAGGGYGGHDDRWCELSVCDHPTPIAELERLYAIYRLTYLRSDPGQLVP
                     LAGELAAEVQAILHERGFLAGYAAGDCDAGTLRALHDFMGWENYDERIRDDGLIDLEV
                     LADMRLRHAAWRASRA"
     gene            complement(8137..9549)
                     /locus_tag="PK144_07605"
     CDS             complement(8137..9549)
                     /locus_tag="PK144_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNF65632.1"
                     /translation="MPELARLPGRLLIVGFGSIGQGVLPLILRHLGVRAADIRIVSAT
                     DDGRAQAEAAGVGFCRCELNPDNLRSVLEPLLAAGDFLLNLSVAVSSLALVRLCRARG
                     ALYLDTCIEPWAGGYADPTLNPAQRSNYALREAALALRAETGPGPTAVLTHGANPGLV
                     SHFTKHALLDLARDTGRPAAPPVSRADWARLAQRLGVRVIHIAERDTQQPTTPKRPGE
                     FVNTWSIDGFAGEGLQPAELGWGSHERRLPPDGHRHARGCDAAIWLARPGASVRVRSW
                     TPLAGPYHGYLITHGESISLADALTVRGPGGSVSYRPTVHYAYHPCDAALLSLHELAG
                     SNWRLQPAQRLIVDEITGGRDELGVLLLGHERIAYWYGSRLDVSAARAAAPHNSATSL
                     QVTAGVLAGMVWAIENPRRGIVEPEDMDHARVLELAAPYLGELVGVYADWTPLAGRGE
                     LFPEATIDDDPWQFANFRVD"
     gene            9767..11266
                     /locus_tag="PK144_07610"
     CDS             9767..11266
                     /locus_tag="PK144_07610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65633.1"
                     /translation="MLSAPTAAPERSPRLLAAITLFVLFDLLVLAVNFWITWQVEHDA
                     LAINVAGRQRMLTQRIAKSLLLLGRPEDATQARRDLRAAKELFGETLAAFEAGGHVRD
                     GDGVEQPFRALEGSDARALVGEARALWQPLAAQIEAALAGAPGALEPAQRAAVRDTEM
                     LLVRMNRLTGVMQAQSNASTVRLRLLQAVVVGLALLNFALIVSLFRQQLRAAAQAGQA
                     LAELIDHLQDGVAVLDGERRIVMANAALGRLFGVEVGRLTGRPVGELLSHEQERDALL
                     GLRPDGARFAAQCTEVDISLHGRPLTLLTIADVTAEQERQAQLRRMAFQDPLTSVPNR
                     LLALDRLESALLRSLRARRRCAVLFIDLDGFKPINDHYGHATGDLVLIHVARVLQAQV
                     RAADTVARYGGDEFLVILPDLQVADDAMTVAAKISAALHGPVRIGPYELPLGVSIGIA
                     SCPEHAATPEALIAAADAAMYRAKRAGGGLIERARPATQSVTDRRSQPG"
BASE COUNT         1591 a         4065 c         4222 g         1590 t
ORIGIN      
        1 ttcagagcgg cgcgaacagc gcgtcgagat cgtcctcggc gagctgcggg gcgctgtcgc
       61 gttcggtcag gatgctctcg accagtgcgc gcttcctgac ctgcaggtcg cggatttttt
      121 cctcgaccgt gccctcggtg tagagcttgt agacgaacac cggcttttcc tgaccgatgc
      181 ggtgcgcacg gtccgaagcc tgatcctcgg cggccgggtt ccaccacggg tcgtagtgga
      241 tcacggtgtc ggcggcggtc aggttcaggc cggtgccgcc ggccttcagg ctgatcagga
      301 ataccggcac ttcgccggcc tggaagcggc gtaccggcgt ggcgcggtcc tcggtggtgc
      361 cggtcagcag cacataggcg atgccgagcc tggtgagttc cttctcgatc agcgccagca
      421 tctgcgtgaa ctgcgagaac accagcacgc gccggccctc gtcgacgagt tccggcagca
      481 tctcggtcag cagttcgagc ttggccgagc ccttgaccgc gcgcgcgctg ccgagcttga
      541 ccagccgcgg gtcgcagcag acctgccgca gcttgagcag cgcgtcgagg atgacgatgt
      601 ggctgcgcgc gaggcccttg gcggcgatct cggcgcgcac gcgctcctgc atcgacaggc
      661 ggatcgcctc gtagaggtcg cgctgcgggc cggcgatttc gatcgtgcgc acgatctcgg
      721 tcttcggcgg caggtcggcg gcgacctgtt ccttggtccg gcgcagcaaa aacggcgcga
      781 cgcgtgccgc gaggcgttcg cgacgttcgc ggtcgccgtc cttctcgatc ggcttgcgga
      841 agctgcgctg gaactggcgg gcatcgccga gcagaccggg cagcaggaag tcgaacaggc
      901 tccagagctc gccgaggtga ttttccatcg gcgtgccggt caggcacagg cggtgccggg
      961 cgcgcagggc gcgggcgacg atgccggcct gcgcggtcgg gttcttgatc gcctgcgcct
     1021 cgtcgaggat cagcaggtga tacgtgtgcc gggcgagcgc ctcgcggtcg cgcggcagca
     1081 gtgcgtaggt ggtcagcacc agatcgtgct cggggatgcg cgcgaaatcg cgggcgcggt
     1141 cggggccgcg cagcgccagc acgcgcaggc gcggcgcgaa gcgtgcggct tcgtcgcgcc
     1201 agttcggcag caggctggtc ggcgcgacga tcagcaccgg ccggtcgagg cggccggcgg
     1261 cgtgctcgca ggcgatgtgc gcgagcgtct gcacggtctt gccgaggccc atgtcatcgg
     1321 cgagcacgcc ggcgagctgc gtgacgcgca ggaactgcag ccattccagg ccctgctgct
     1381 ggtacgggcg cagctgggcg tgaaagccct ccggcaccgc gaccggctgc ggaccgcgcc
     1441 gcagttcggt gagcagcgtg cgcaggcgtt cgccgccgtg ccaggtgagc gtgcggtcgc
     1501 cgagtgcggc ttcgagctcg gcaagccgcg ttgcctggct gcgcgcgagc gacagcttgc
     1561 cgcgcaggtt caggccctcg ccgtcgtaga gctcgaccag caccgcgagg atcttgcgga
     1621 tgcgcgccgc cggcaccggc agcagccggc cgtcgggcag cggcaccatg acccggcgct
     1681 cgtcgccgag ctgcgcgagc cagctgcgcg cgtcgccgcc gggcaggcgc gcgagcagct
     1741 cgaccagaca gggcagcagg ttgacgcgct cgccgtcgag ctcgacgccg agggccagtc
     1801 cgaaccagtc ctcgccgccg ctctcgtcga gttcggcgaa ccattcgccg ggttcggcga
     1861 ggtcgtagcg gaagcccggc tcgatctcga tgcgccagcc ctcggcacgc agccgcggca
     1921 gttcgccgag cacgaaatcg gcccagcggt ccgggtcatg gaagccgaag tcgtgcctgc
     1981 agctgcgcgg caggcgctcg gcggcccagc tcaggttgcg cagcgcgatc aggtcggcgt
     2041 cgtacaggcg ctcgatcgcg gcgcgttcga ggtcgcgccg ccgggcgatg cgctcgacca
     2101 cgcgatcggc acgcaggcgg ctgatcaccg gcgtgtcgtc gtgccagtcc agccagtggg
     2161 catcgccgta gccgaagtcc agatgcacgc catcgacgag gccggtgccg ctgcgcgtgc
     2221 cgcggccgat gccggcggcc ggcgcggcgc cgagttcgcg gctgtacagg cgcaggcgcg
     2281 gctgcggacg gaagtcggcc tcggtcgcga cctgcagccg gggcgggcgc ggcagcgcga
     2341 cgccgggcag gtggcgctcc agccagtcgt tgagcgggtc gatgtcctcg gcttcgagca
     2401 ccggtgccga ggccagcgcc ccggccagcg ccggcggcag cgtggtgctg agcggcccgc
     2461 agcagccggc ctgcgcatcg acgtaccacg gcggcgacag cggcagcacc gcatcggccg
     2521 gcgggccggc gacgaaggcg agccgggtgc cgccgccggg atcggtctgc cactgcacgg
     2581 tggccgggcg ggccgggcct tcggcgagca gcggcccgtt ctcgtcgagc cagtggcagc
     2641 gcccgctcgc cagcacgcgc gcgagcagcg ccgcgccgcg ctcgccggcg aggccgagcg
     2701 tgcgggcgta cgggtcgcgc gccagcgcca gttcgtgcag cagcgcgcgg tcggcatcct
     2761 cgaagcggcc gggatacatg ccgggcacca gcaccgagcc cggatcgagg ttgcgcggct
     2821 tgccgtatcc gccctttttg agcgcgcgcg ccgagaccac gcgcagcgcg caggcgtcgg
     2881 gggcatcgcc ctgcggctgc agcaggtaca gcaggcgttc ggcggcatcg ccggccggct
     2941 tcggcagcgc ggcgtggccg acgcgttcga gccaggcgga cagcgccggc gagggaccgc
     3001 tcgccggggc cggcgtcacc ggcgcgagcg gcggcacgca ggccgggcgg tcgctgccgc
     3061 tgccgagggc cagcaggcgc agcagcagcg cggcggcgtg cttgcagtcg acgccgaccg
     3121 gacacgagca ctcggtttcg agctgcaggc cgcgggtgtt ctggatcagg cgcacgcgcg
     3181 tctcgtagcg cgggcggtgg ctgccgcgca cgacgccgtg cacgctgccg tcggtgccgg
     3241 ggcgcagctc cagcacgccg ccggtgcgca gcagggcctc gccgcgcacc agcgtcagcg
     3301 ggtcgaacca gtggcgcagc agatcccggc tgggatgcgc ccgcgtgccg gggccggaca
     3361 gactcgtgtc gatcatcgca gcagcaacat gtggagggcc gtcccggccg cgatgctgag
     3421 cagcgcattg cgccgggcga gatggacggc cacggttacg acgacgcccg cgagcgcggg
     3481 caacggcccc ggagccccgc tcgtgaacgt atccctgagg caatacagca ccagaacggt
     3541 catgaccgcc ggtggcagat aacgtgcgag gaagcgcagc cacggccgct cgccggcacc
     3601 ggcgaacagc aggaacggcg cggcccgggt cgcgaaggtc gcgagcgcca tcagcgccac
     3661 ggccgcgagc acgtagctca tgccggtcgt gcccgctgca cggccagcag cccggccagc
     3721 gtcagggcca gcgccaccag caggaactgc gcaggcggtg cgaacgcgaa gctgacggca
     3781 ccggcggacg cggcgagcaa aaacggccac ggctcgcgca ccgcgtgcca ctgctcgacc
     3841 agcagcacga cgaacagcgc ggtcagcgtg aattcgagcc cgtgcgtgtc gagcccgccg
     3901 agcgcgagtc cgccgagccc gccgagcgtg cagccgagca gccaccagcc ctggttcagc
     3961 gcgctgaccg cgaacacgaa atcgtgcgcg gccggcgggg tggcgaaggc cggtggcggg
     4021 cgcgtgccgg tcagcagcga ccaggtttcg tcggtcagcg cgaacaccgc ataccagcgc
     4081 cgccagccgc gcaccggcag ccggtcgatc atcgacaggc catagaaggc atgacgcagg
     4141 ttcagcagca gcgtcgcgag tgcgatgtcg gcgagcggca cccgggccgc gagcaggccg
     4201 accgcgagga actgcgcggc cccggcgtag atgcagagcc ccatcagcgg ggccagccag
     4261 gccggctggc cgctctgcac cagcagcacg ccgaacgcca tcccgagcgg cacatagccg
     4321 aacgccaccg gcagacaggt gcgcagggcg gcagcgagca cgtcggggct gggcatgtgc
     4381 ggcatccgga atgggggcga tccaaagtac cggatagtaa ctaaatacat ggctatcgtg
     4441 tgggaatacc gcagtgccgc aacgcgcgtt cctgccgcgc atcccggccc tctgcggcgc
     4501 ggagcattcg attgcgggct tgcgaccatg cagttatttt gaaatccggt ctgaccgcgc
     4561 ctccctgccg cgttggccgc ccgcaacagt caaatcgcgg atcgtcgctg caaggcgaca
     4621 atgcaagtcc ctgacggata tctgatcttt tccgcatccg gtccgctcgt cgtcgaaagg
     4681 cgacgaatct ccccgtgaat tccgcgatgt ccaggccgca tggcccggtt ttttctggaa
     4741 atttcggtgt tggcactgcg attgctttgg ctgatgccaa gggaggcgcg agtcgcggtc
     4801 cgggtttaag aacggccccg tccccgatcc ggccgcggct cgcgcacccc tcctgacgtg
     4861 acagccgtcg gcagcggtac tgccgcaatt cgtctcctcc agaggagtca aaaatcatga
     4921 acaacaaact catggcctgc ctgctggcgg cgacgctggc gctgccgctg agcgcgccgg
     4981 cgcaggccgg cagcggcgat gcgctgatcg gcggtggcgt cggtggtgcg ctcggtgcgg
     5041 tggtcggcca cgagatcagc ggtcgcgacg gcgcgatcgt cggcggcgcg ctcggcgcgg
     5101 cgctcggcgc ggcggccacg acgcagggtg atcgcgatta ccgttaccgg cgcgtctaca
     5161 acgggccggt ctacgaacgt ccgtatgtcg tcgagcggcc gtactacgcc ccgccgccgc
     5221 cgcgctatta cgcgccgccg ccgcgctacg tcgtcgagcg ccgcgtctac gtcgaggatc
     5281 gcggctatcg ccgcggcccg ccgccgtggg cccggcacca ccatcgccat caccggcact
     5341 ggcgcgactg ggactgaata cccgctgagc gctgccggtt ctctgcgacg ccgcccccag
     5401 actccgggcg gcgttttttt gtgcgtgcgt cgcgccggcc gaccacactg caggcccccg
     5461 cccgcatcgc cgtcttctgc aagctgcctg cgcacccttc tttcgcaacc tccatccgca
     5521 acctccatcc gggagtcgtt ccatgtctgc cgtcccgcgc ctgcgcgccg ccggtcttgt
     5581 gctgatcggc ctgaccgcca cccttgtctc ggcctgcggg ggcggcggcg gtccgtacgc
     5641 cggcgcaccg gtcgccggcc tgccgccgcc gaaggtgacg ggcgcactgg cgggcgggat
     5701 gtcgggggcg ctgctggcga aggccaccga accgcgcggc gcaccggcgg cgctgccggc
     5761 cgcgcagttc ccgcaggccg gcaagagccc ggtggtgcgc acggccgaac agccggtctc
     5821 gaccttcagc ctcgatgtcg acacggcctc gtatgcgctg gtgcgccggc tgctcaacga
     5881 cggccggctg ccgccggtcg atgcggtgcg catcgaggag atggtcaact acttcccgta
     5941 cgcgtggccg gcgcccgagg cccgcagccg gccgctgcag gtgtcgacgg cgctgctgcc
     6001 gacgccgtgg aacccggaca cgcgcctgct gcgcatcggc atccggggct gggcgccggc
     6061 cacgcacgag cggccgccgg cgaatctggt gttcctgatc gacgtgtccg gctcgatgga
     6121 cgcgcccgac cgcctcgggc tgctgcagaa gtcgctcggc ctgctggtcg agcggctgcg
     6181 gcccgaggac cgcgtcgcga tcgtcagcta cgccgggggc gtgcgcacgg tgctggagcc
     6241 gacctccgga cgcgagcgca cgaagatcct cgccgcgctc gatggtctcg ccgccggcgg
     6301 cagcacggcc ggggcggccg ggctggagca ggcctaccgg ctggcgcgcg agcacttcga
     6361 ccggcaggcg gtcaaccgcg tgatcctcgg caccgacggc gacttcaacg tcggcctgtc
     6421 cgatccggcc gggctcgcgc agtacgtgag cgagcagcgc aaggcgggcg tgtacctgtc
     6481 ggtgctgacc gtcggtctcg acaacctgca tgacggcacg gcgcaggcgc tggcgcaggc
     6541 cggcaacggc aatgccgcct atctcgacag cgtccaggaa ggccggcgcg tgctggtcga
     6601 gcagctcgcg gcgacgatga ttccggttgc cgacgatgcc aaggtgcagg tcgagttcaa
     6661 cccggccgag gtcagcgaat accggctgct cggctacgag acgcgcgccc tggcgcgggc
     6721 cgacttcacg aacgatgccg tcgatgccgg tgacctcggc gtcggccaca cggtcacggc
     6781 gctctacgag atcacgccgg ccggcgcccg ggcgcgcatc gagcccgggc gctacgaggc
     6841 accggcggcg acggcgccga accgccatcc cggcgagctc gcctgggtga agctgcgctg
     6901 gaagcagccg ggcgcggcgc gcagcgaagc cctcgcgcag ccggtgcgtg cggccgacgc
     6961 cttcgccggc atcgccgccg ccgatgccga cacgcgcttc gcggtcgcgg tcgccgcctt
     7021 cggacagtgg ctgcgccagg acacggagct gcgcggctac ggcatcgacg cgatcgaaac
     7081 cctggcgcag ggcgcacgcg gcgaggatgg cgagggccgg cgggccgagt tcgtgcagct
     7141 cgtgcgccag gccgcggcac tgcgcggcgc gccggcgcgc tgaggccgca ctacgctgcg
     7201 caaggcggcg ccgtgccgcc cgatgcccct gcggagtgcc tgcccatgac cttctcgatc
     7261 gtcgcccacg atcccgagac cggcgacctc ggcgtcgccg tgcagtcgaa gttcccgaac
     7321 gtcggcgtgt cgatcccgta cgcgcaggcc ggtgtcggcg cgatcgcgac gcagtgctac
     7381 tgcaacacca gcttcgggcc gcgcgggctg gcgctgctcg gtcacggcgc gagtccgcag
     7441 caggcggtcg agatcctgac cggcaacgat gccgagcgcg aattccgcca gctcggcatc
     7501 gtcgatgtgc aggggcgcgc cgcgagcttc accggcgcgc gctgcttcga ctgggccggc
     7561 agccgccacg ccgcgcacgt cgcggtgcag ggcaacgtgc tcgccggggc cggcgtgctc
     7621 gatgcgatgc tggagcgcta ccaggccggg gccggcgagc cgctgccgct gcgcctgatc
     7681 gccgcgctcg ccgccgggca ggcggccggc ggcgaccgcc gcggccagca gtcggcggcc
     7741 gtcaaggtcg tgcgtgccgg gggcggctac ggcggccacg acgatcgctg gtgcgagctg
     7801 tcggtctgtg accacccgac gccgatcgcc gagcttgagc gcctgtacgc gatctaccgg
     7861 ctcacctacc tgcgcagcga ccccgggcag ctcgtgccgc tcgccggtga actcgccgcc
     7921 gaggtgcagg cgatcctgca cgagcgcggc ttcctcgccg gctacgccgc cggtgactgc
     7981 gatgccggca cgctgcgcgc gctgcatgat ttcatgggct gggagaacta cgacgagcgc
     8041 atccgcgacg acggcctgat cgatctcgaa gtgctggccg acatgcgcct gcgccatgcc
     8101 gcgtggcgcg cgagccgcgc ctgaggcggg cagggttcag tcgacgcgaa agttggcgaa
     8161 ctgccaggga tcgtcgtcga tcgtggcttc cgggaacagc tcgccgcgcc cggcgagcgg
     8221 cgtccagtcg gcgtagaccc cgaccagctc gcccagatac ggcgcggcga gctccagcac
     8281 gcgggcgtga tccatgtcct cgggctcgac gatgccgcgc cgggggtttt cgatcgccca
     8341 caccatgccg gccagcacgc cggccgtgac ctgcaggctg gtggcgctgt tgtgcggtgc
     8401 ggcggcgcgc gcggcgctca cgtcgagccg cgaaccgtac cagtaggcga tgcgctcgtg
     8461 gccgagcagc agcacgccga gttcgtcgcg cccgccggtg atctcgtcga cgatcagccg
     8521 ctgcgccggc tgcagccgcc agttgctgcc ggcaagctcg tgcagcgaca gcagcgcggc
     8581 atcgcagggg tgataggcgt aatgcacggt cggccggtag ctgacgctgc ctccggggcc
     8641 gcgcacggtc agcgcatcgg cgagcgagat cgattcgccg tgcgtgatca ggtagccgtg
     8701 atacgggccg gcgagcggcg tccagctgcg cacgcgcacg ctcgccccgg gtcgcgccag
     8761 ccagatcgcc gcatcgcagc cgcgcgcgtg ccggtggccg tcgggcggca gccggcgctc
     8821 gtggctgccc cagccgagtt cggccggctg caggccctcg ccggcaaaac cgtcgatcga
     8881 ccaggtgttg acgaactcgc cggggcgttt cggggtcgtc ggctgctgtg tgtcgcgttc
     8941 ggcgatgtgg atcacgcgca cgccgaggcg ctgcgcgagc cgcgcccagt cggcgcgcga
     9001 gaccggcggt gcggccggcc ggccggtatc gcgggccaga tcgagcagcg cgtgcttggt
     9061 gaaatgactg accagccccg gattggcgcc gtgcgtcagc accgcggtcg gaccgggacc
     9121 ggtctcggcg cgcagcgcca gggccgcctc gcgcagcgcg tagttgctgc gctgggccgg
     9181 gttcagggtc gggtcggcgt agccgccggc ccagggttcg atgcaggtgt cgagatacag
     9241 cgcgccgcgt gcgcggcaga gtcgcaccag cgccagcgac gatacggcca ccgacaggtt
     9301 cagcaggaag tcgcccgctg ccagcagcgg ttcgaggacg ctgcgcaggt tgtcggggtt
     9361 cagttcgcag cggcagaagc cgacgccggc ggcctcggcc tgggcgcggc cgtcgtcggt
     9421 ggcgctgacg atgcggatgt cggccgcacg cacgccgagg tggcgcagga tcagcggcag
     9481 gacgccctgg cctatgctgc cgaagccgac gatcagcagg cgtccgggca gacgtgcaag
     9541 ttccggcatc tggaattcca cgtatttcgc agggtcgatg gcgggagcat gaccaatccg
     9601 ccgcagcccc gccaccccgg cgagtccctg accgagtccc tgaccaaagc gtatgctgcg
     9661 gtgcagcacg attatcgtta cagtggattc atatagaagc agccacactg ccagcaccgg
     9721 cagccgatgc cggttgtccc cgcgtcatcc ggagtgacga tcgaccatgc tgagcgcacc
     9781 gaccgccgcg cccgaacgct cgccgcgcct gctcgccgcc atcacgctgt tcgtgctgtt
     9841 cgacctgctg gtgctcgcgg tcaacttctg gatcacctgg caggtcgagc acgatgccct
     9901 ggccatcaac gtcgccggcc gccagcgcat gctgacgcag cgcatcgcca aatcgctgct
     9961 gctgctcggc cggcccgagg atgccaccca ggcccgtcgc gacctgcgcg ccgccaagga
    10021 gctgttcggc gagacgctcg cggccttcga agccggcggc cacgtgcgcg atggcgatgg
    10081 cgtcgaacag ccgttccggg cgctggaggg cagcgatgcg cgcgcgctgg tcggcgaggc
    10141 ccgggcgctg tggcagccgc tggccgcgca gatcgaggcg gcgctcgccg gtgcgccggg
    10201 ggcactggag ccggcgcagc gcgccgccgt gcgcgatacc gagatgctgc tcgtgcgcat
    10261 gaaccgcctg accggcgtca tgcaggcgca gtcgaatgcg agcacggtgc ggctgcgcct
    10321 gctgcaggcc gtggtcgtcg ggctggcgct gctgaacttc gcgctgatcg tctcgctgtt
    10381 ccgccagcag ctgcgggccg cggcgcaggc cggccaggcg ctggccgagc tgatcgacca
    10441 tctgcaggat ggcgttgcgg tgctcgacgg cgagcggcgc atcgtcatgg ccaatgccgc
    10501 gctcgggcgg ctgttcggcg tcgaggtcgg gcgactgacc ggccgcccgg tcggcgagct
    10561 gctgagccat gagcaggagc gcgacgcgct gctcggcctg cgccccgacg gcgcacgctt
    10621 cgcggcacag tgcaccgagg tcgacatcag cctgcacggc cggccgctga cgctgctgac
    10681 catcgccgat gtgaccgccg agcaggagcg ccaggcgcag ctgcggcgga tggctttcca
    10741 ggacccgctg accagcgtgc cgaaccggtt gctggcgctc gaccggctcg aatcggcgct
    10801 gctgcgcagc ctgcgcgcgc gccgccgctg cgcggtgctg ttcatcgacc tcgacggctt
    10861 caagccgatc aacgaccact acggtcatgc gaccggcgat ctggtgctga tccacgtcgc
    10921 gcgcgtgctg caggcgcagg tgcgggccgc ggacacggtc gcgcgctatg gcggcgacga
    10981 gttcctcgtg atcctgcccg acctgcaggt cgccgatgac gcgatgaccg tggccgcgaa
    11041 gatcagtgcg gcgctgcacg gcccggtgcg catcggtccg tacgagttgc cgctcggcgt
    11101 cagcatcggc atcgccagct gcccggagca cgcggccacg cccgaggcgc tgatcgccgc
    11161 cgccgacgcg gcgatgtatc gcgccaagcg cgccggcggc ggcctgatcg aacgggccag
    11221 acctgcgaca caatctgtca ccgaccggcg ctcgcagccg ggctgaactt ttccgggcct
    11281 gcaggcgtcc atcgtcatcg agcggatgtt cttcgcttgc ccgatcccct ccctggtcgg
    11341 gcggacccgg ccccccctgc cgggtcgtct tggagccccg gaccctcccc ctgtaccggg
    11401 gcttttttat gcacggcttt ccgggcgctc ttgaaaccgc tgccgcagcg ccgcatttcg
    11461 acggttcg
//
LOCUS       DAOQIE010000095        11457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2802_length_11457_cov_7.127872, whole genome shotgun sequence.
ACCESSION   DAOQIE010000095 DAOQIE010000000
VERSION     DAOQIE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2802_length_11457_cov_7.127872"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK144_07615"
     CDS             complement(<1..308)
                     /locus_tag="PK144_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65634.1"
                     /translation="MNMPAAPQFPLTLMYAVPAAAALAALAPSAAAAVQSALLDAAAG
                     RLKLAMAPGRREGVLAAAGRRVLCQPHGARLMVLLLAAAPPGDGPATARSVREVRMII
                     "
     gene            473..1249
                     /gene="pssA"
                     /locus_tag="PK144_07620"
     CDS             473..1249
                     /gene="pssA"
                     /locus_tag="PK144_07620"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNF65635.1"
                     /translation="MSQPTPETPVTEIKRPRGIYLLPNLFTTAALFAGFYGVLAAMNG
                     RYEAAAVAIFVAMILDGLDGRVARMTHTESEFGVQYDSLSDMVSFGVAPGLIMYQWTL
                     SGLGKAGWLAAFVYTAGAALRLARFNVQVGSIDKRYFMGLPSPSAAAVTMGLVWIGTD
                     LGLDPATLELPAFVLTLGAGLLMVSNFLFHSFKKIDLNGKVPFVVVTVVMLGFGLAYL
                     QTPAMVFGGFLIYALSGPTLAIVRWRRRRRRHEPDAGSGG"
     gene            complement(1238..2035)
                     /locus_tag="PK144_07625"
     CDS             complement(1238..2035)
                     /locus_tag="PK144_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNF65636.1"
                     /translation="MLELFLLPPGGPLCLALLGLLLRLRRPRLGSALITSGLLAAWLL
                     STPQMAATLNRALAPPPVLSDEAIRESGAEAIVVLAGGLSVRAPEYGPIDLNAITLAR
                     LRYGARLQRLTGLPVLVSGGYRDHGREPSEAARMAAVLSEEYGVREVWTEDRSQNTRE
                     NAAYSAPMLAAQGVQRVLLVSHAAHLPRALEAFARAGVDAVPAPTQSFLWTPPPGVEA
                     TTEAADWLPQAGAAWEAWYASHELLGRLWYRLRAALTPAPEAAPSAT"
     gene            complement(2129..3244)
                     /locus_tag="PK144_07630"
     CDS             complement(2129..3244)
                     /locus_tag="PK144_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNF65637.1"
                     /translation="MSSVLRRLCCLLAALAALLPAARAAEVPRALVLPPGFTIELWAE
                     LEDARTLVLGGDGRTVYVGSRRAGTVTALRDTDGDGRADARWTLATGLTMPNGLAVRG
                     DALYVADVDRLLRFARVEASFGQPVAPQVLRKDLPRDSAHGWRYLAFGPDGKLYLSIG
                     APCNVCEAPGYAELRRMNAEGRDEETVARGLRNSVGFAWRPGTRELWLTDNGRDWLGD
                     DQPPDEIDILITPGAHFGFPYCHGGRLPDPQYGRGRSCDDYSAPALRLDAHVAPLGIL
                     FYGGRQFPAEYRGRVFVAEHGSWNRSRKSGYRVMMADVVDGRPEDYRPFLIGFERDGE
                     VSGRPAYLLELPDGSLLLSDDQGGRIYRIRYSGGSGG"
     gene            3387..3632
                     /locus_tag="PK144_07635"
     CDS             3387..3632
                     /locus_tag="PK144_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNF65638.1"
                     /translation="MSLPPHDFPHLVHELPEFRERIRVLQAEDAHFARLYERYLDVSR
                     ELHGLGQDGEPLTGQAAVLASRRVHLKGVLYEMLRGD"
     gene            3710..4351
                     /locus_tag="PK144_07640"
     CDS             3710..4351
                     /locus_tag="PK144_07640"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNF65639.1"
                     /translation="MSHPDSHSDDERRRTLADELIEAGRFFFARGWVPATSGNFSARA
                     GDDDVLITVSGRHKGRLGTDDLMHIDLHGRPARAGQRSSAETGLHLQLYRRDPAIGAV
                     LHTHSVNATVLSMLAGGALVLRDLEVLKAFPGIATHESAVCVPVLANDQDIARLAARV
                     AEVLDRDPGCCGYLIAGHGFYTWGATVADAVRHVEAFEFLFECESLMRRMGRP"
     gene            4348..4893
                     /locus_tag="PK144_07645"
     CDS             4348..4893
                     /locus_tag="PK144_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HNF65640.1"
                     /translation="MSKLRVYRDDAPDTPIVEHTLGADIALELEGPEVLFSRWEADRE
                     LPADASNDDIIGAYRAQIDVLMTRYGFHAVDVVNVTPTHPDKDALRQKFLSEHTHSDY
                     EVRFFVEGSGQFYIHHADRVYMIFCQAGDLLSIPANMTHWFDMGPNPSFKCIRLFTTP
                     EGWVANFTGSDIAEHFPRLVN"
     gene            4916..5605
                     /gene="mtnC"
                     /locus_tag="PK144_07650"
     CDS             4916..5605
                     /gene="mtnC"
                     /locus_tag="PK144_07650"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNF65641.1"
                     /translation="MAIRAILTDIEGTTSSIAFVHEVLFPYARARLPAWLAAHAGEPE
                     VAGQLAATRAEAGLPPEAPLAQVIEVLLGWIDADRKATPLKALQGRLWADGYAAGDFH
                     GHVYADVPPCLRAWQAAGLVLAVYSSGSVQAQQQLFAHTACGDLTPLFSAYFDTRIGH
                     KREPASYTAICAALGLAPAGVLFLSDITEELDAARSAGLATVQLVRDGTPDPQAAHRQ
                     VTAFTAIDPYA"
     gene            5721..6512
                     /locus_tag="PK144_07655"
     CDS             5721..6512
                     /locus_tag="PK144_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65642.1"
                     /translation="MTPETPEPVPALEIVDLHKCFGSLEVLKGISLSARDGDVISVLG
                     SSGSGKSTCLRCINLLEQPTSGTIRVKGETLKLARDRGGQLASTDPKQLARIRSRLAM
                     VFQSFNLWAHMTVLDNLIEAPVHVLKLGRAEAIERAERLLHKVGLYERRNYYPAHLSG
                     GQQQRAAIARALAMEPDAILFDEPTSALDPELVGEVLKVMRVLAEEGRTMIVVTHEMG
                     FAREVSSRVVFLHQGRVEEDGPPAEVFGHPRSERCRQFLSGKLKG"
     gene            6588..7379
                     /locus_tag="PK144_07660"
     CDS             6588..7379
                     /locus_tag="PK144_07660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF65643.1"
                     /translation="MKKIALAALGCVLALGIGFGSVADAKEWKKVRIATEGAYPPFNF
                     VDADGKLAGFEIDLANALCAEMKVECTLVKQDWDGMIPALKARKYDLIMASMSITDER
                     KQQVDFSDWYYRMPARFVAKKGAGLTVTPEGLKGKTIGVQRETVSDKFLTDNYGSSVT
                     IKRYATQDEVYLDLTAGRLDAYVADIIVSRDAFLSKPEGKDYEFTGPEFSDPKWFGDG
                     IGAAMRKQDNDLTALFNKALKALIANGTYQKVAAKYPDFDSYLRK"
     assembly_gap    7381..7480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7504..8193
                     /locus_tag="PK144_07665"
     CDS             7504..8193
                     /locus_tag="PK144_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF65644.1"
                     /translation="MLDFHGYGASVFEGAWLTLEVALMSLVVAVALGLAGALAKLSRS
                     LVLRTIAGLYTTIIRGMPDLVLMLLIYFGGQTLVNVISDALGWEYIDINPFVAGVLTI
                     GLIFGAYMTETFRGALMAVPKGQLEAGYAYGMSATQVFVRIHLPQMLRYALPGFGNNW
                     LVLLKTTALVSVIGLDDIVRKAQLAAGATRLPFTFYGAVLLGFLAFTTVSVLLLRWAE
                     RRYSLGYERME"
     gene            8200..8892
                     /locus_tag="PK144_07670"
     CDS             8200..8892
                     /locus_tag="PK144_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019933499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF65645.1"
                     /translation="MEFGVILDNWPLYLEGLVTTVELTGLCLILGLLLAIPLGTVQAV
                     GPLWARGLVFAYSYVFRGTPLLVQTFLIYYGLGQFEAVQQSVFWPLLKEAYWCALIAF
                     TLNTGAYTTEIIRGSIVTMPRGEIEAARACGMDTLLMLRRIVLPSAFRRALPAYGNEV
                     IFMLQGSSIASTITLVDLTGAARVINSRYYLPYEAFLTAGLLYMALSFLLFAGFRKLE
                     QRWFAHLRPREH"
     gene            complement(8952..9722)
                     /locus_tag="PK144_07675"
     CDS             complement(8952..9722)
                     /locus_tag="PK144_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HNF65646.1"
                     /translation="MTAVAAKPEGLLIDLAGVLHVNDRALPGAAETLARLAAAGVPHA
                     FVTNTTRQTRTAIAAQLAGLGLPVPESRIVSAVDATHDWLAVRRLRPHLLVHPAVIAA
                     FADLEQESPDVVVLGDAADGFHYENLNHALQLLLGGAQLVAMADNRYFRDGERLALDI
                     GPFRAALEYASGVSATIIGKPAQAMFETGCARLGLPPQRVAMIGDDAEADVAAALAAG
                     LGAAVLVRTGKYRDGDERKCPRAGLADDFAAAVEMLLD"
     gene            9888..11303
                     /gene="thrC"
                     /locus_tag="PK144_07680"
     CDS             9888..11303
                     /gene="thrC"
                     /locus_tag="PK144_07680"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNF65647.1"
                     /translation="MQYLSTRGDAAARTFSDILLAGLAPDGGLYLPEHYPQVSAAELA
                     AWRELPYAELAFEILRKFATDLPEADLRALTAKTYTAAVYRSDAITPLRTLEPGLYIL
                     GLSNGPTLAFKDMAMQLLGNLFEYVLGQSGEELNILGATSGDTGSSAEYAMRGKRGIR
                     VFMLSPHGKMSAFQTAQMFSLLDPNIHNLAVRGVFDDCQDIVKAVSNDHAFKARHRIG
                     TVNSINWARVVAQVVYYFRGYFAATRSNDEEVAFAVPSGNFGNICAGHIARQMGLPIR
                     RLVLATNENDVLDEFFRSGVYRPRASAETWHTSSPSMDISKASNFERFVFDLMGRDAA
                     KVRELWARVDAGGAFDLSGTPEFAAITGRYGFVSGSSTHADRLATIRDTAARFDDVID
                     THTADGLKVAREFTEPGVPMIVLETALAAKFEETIIEALGRKPGRPDGYERLESLPQR
                     FEVIDADAAQVKAYIARHVGG"
     gene            11381..11457
                     /locus_tag="PK144_07685"
     tRNA            11381..11457
                     /locus_tag="PK144_07685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11415..11417,aa:Arg,seq:cct)
BASE COUNT         1721 a         4059 c         3879 g         1698 t
ORIGIN      
        1 tgtatgatca tgcgtacctc ccggacagac cttgccgtcg ccggaccgtc gccgggtgga
       61 gccgcggcga gcagcagcac catcagccgc gcaccgtgcg gctggcagag cacgcgccgg
      121 ccggcggcgg cgagcacgcc ctcgcgccgg ccgggcgcca tcgcgagttt cagccggccg
      181 gcggcggcat cgagcagggc gctctggacc gcggccgcgg cggatggcgc cagggccgcc
      241 agcgcggcgg cggcagggac ggcatacatc agcgtcagcg ggaactgcgg ggcggcgggc
      301 atgttcatga gtgaatagcc tgcgcgagcc gccggcggcc ggcaaggcgg tgtgcggtgg
      361 cgcccgcgcc ggcgcgcacg ccataatccg ccgcccgccc ggcggcgcgt gccctttccg
      421 cgccctcccg cgcccctccc gcgcctgcat gcgcgccgca ccggatcgcc ccgtgagcca
      481 gccgacgccc gagacccccg tcaccgaaat caagcgcccg cgcggcatct acctgctgcc
      541 gaacctgttc acgaccgcgg cgctgttcgc cggcttctac ggcgtgctgg cggcgatgaa
      601 cggccgctac gaggccgcag ccgtcgcgat cttcgtcgcg atgatcctcg atggtctcga
      661 cgggcgcgtg gcgcgcatga cgcacaccga aagcgagttc ggcgtgcagt acgacagcct
      721 ctccgacatg gtctcgttcg gcgtcgcgcc ggggctgatc atgtaccagt ggacgctgtc
      781 gggcctcggc aaggccgggt ggctggcggc cttcgtgtat acggccggcg cggcgctgcg
      841 tctggcgcgc ttcaacgtgc aggtcggcag catcgacaag cgctacttca tgggcctgcc
      901 gagcccgtcg gcggcggccg tgaccatggg gctggtgtgg atcggcaccg atctcggcct
      961 cgatccggcg acgctggagc tgccggcctt cgtgctgacg ctcggtgccg gcctgctgat
     1021 ggtcagcaac ttcctgttcc acagcttcaa gaagatcgac ctcaacggca aggtgccctt
     1081 cgtcgtcgtc accgtcgtga tgctcggctt cgggcttgcc tacctgcaga cgccggcgat
     1141 ggtgttcggc ggcttcctga tctacgcgct gtccgggccg acgctggcca tcgtgcgctg
     1201 gcgccgccgc cgtcgccgtc acgagcccga cgccggctca ggtggctgaa ggcgcggctt
     1261 cgggcgccgg cgtcagggcc gcgcgcagcc ggtaccacag ccggccgagc agttcgtggc
     1321 tcgcatacca ggcctcccag gccgcgccgg cctgcggcag ccagtcggcc gcttcggtcg
     1381 tggcttccac gcccggtggc ggcgtccaca ggaaggactg cgtcggggcc ggcaccgcat
     1441 cgacgccggc gcgcgcgaag gcttcgagcg cgcgcggcag atgcgcggcg tggctgacca
     1501 gcagcacgcg ctgcacgcct tgcgcggcca gcatcggcgc gctgtaggcg gcgttctcgc
     1561 gggtgttctg cgagcggtcc tcggtccaga cctcgcgcac gccgtattcc tcgctgagca
     1621 ccgccgccat gcgcgcggcc tcggacggct cgcggccgtg atcgcggtag ccgccgctga
     1681 ccagcaccgg cagcccggtc aggcgctgca gccgggcgcc gtagcgcagc cgcgccagcg
     1741 tgatcgcatt caggtcgatc ggcccgtatt cgggcgcgcg cacgctcagg ccgccggcga
     1801 gcacgacgat ggcctcggcg ccgctctccc ggatggcttc atcggacagc accggcggcg
     1861 gcgccagcgc gcggttcagc gtcgccgcca tctgcggcgt cgacagcagc caggcggcga
     1921 gcaggccgct cgtgatcagc gcgctgccga gccgcggccg gcgcaggcgc agcagcagcc
     1981 cgagcagcgc gaggcacagc gggccgccgg gaggcagcag gaagagttcc agcatcggtc
     2041 gcgtgtcctc agcaggccag aaagggcggc cgaggattcc agaggccggc gccccgcgac
     2101 aagcgccgat gcgcgccggc gacgggtttc agccgccgct gccgccgctg tagcggatgc
     2161 ggtagatgcg cccgccctgg tcatcggaga gcagcagcga gccgtccggc agttcgagca
     2221 ggtaggccgg ccggccgctg acctcgccgt cgcgctcgaa gccgatcagg aagggccggt
     2281 agtcctcggg ccggccatcg accacgtcgg ccatcatcac gcggtagccg ctcttgcggc
     2341 tgcggttcca ggagccgtgc tcggcgacga acacgcgccc gcggtactcg gccggaaact
     2401 gccggccgcc gtagaacagg atgccgagcg gcgcgacgtg ggcgtcgagg cgcagcgccg
     2461 gggccgagta gtcgtcgcag ctgcgcccgc gcccgtactg cgggtccggc aggcggccgc
     2521 cgtggcagta cgggaagccg aagtgcgcgc ccggcgtgat caggatgtcg atctcgtccg
     2581 gcggctggtc gtcgccgagc cagtcgcggc cgttgtcggt cagccagagt tcgcgcgtgc
     2641 cggggcgcca cgcgaagccg acgctgttgc gcaggccgcg ggcgacggtt tcctcgtcgc
     2701 gaccttcggc gttcatgcgc cgcagctcgg cgtagccggg cgcctcgcag acgttgcacg
     2761 gcgcgccgat cgacagatac agcttgccgt ccgggccgaa ggcgagataa cgccagccgt
     2821 gggcgctgtc gcgcggcagg tccttgcgca gcacctgcgg cgcgaccggc tggccgaagc
     2881 tcgcctcgac gcgcgcgaag cgcagcagcc ggtcgacatc ggcgacgtac agcgcatcgc
     2941 cgcgcacggc gagaccgttc ggcatggtga ggccggtggc cagcgtccag cgcgcatcgg
     3001 cgcggccgtc gccatcggtg tcgcgcagcg ccgtgaccgt gccggcgcgg cgcgagccga
     3061 cgtagacggt gcgtccgtcg ccaccgagca ccagcgtgcg ggcgtcctcc agctcggccc
     3121 agagctcgat cgtgaagccc ggcggcagca ccagggcgcg ggggacttcg gcggcgcggg
     3181 ccgcaggcag cagcgcagcg agcgcggcca gcagacagca gagacggcga agcactgagg
     3241 acatctgcgg actccgatga aagcgggggg gcggccggag ctttgaccgc gatcaggaca
     3301 agccgggcat gcctgtgatg caatgcggtg tcaggcacag ggcgcttgcg cccatgcaca
     3361 gcgccagaaa gaaggatatt gatcgcatgt cactcccccc gcacgacttc ccgcacctcg
     3421 tgcacgaact cccggaattt cgcgagcgca tccgggttct gcaggcggaa gacgcacact
     3481 tcgcacggct ctacgaacgc tacctcgacg tgagccgcga gctgcacggc ctcgggcagg
     3541 acggcgagcc cctcaccggt caggccgcag tgctggcgag ccggcgcgta cacctgaagg
     3601 gcgtgctcta cgagatgctg cgcggcgact gagggagcgg atgcacgggt ttacggccgc
     3661 cggagccttg actacagtgc tgcgccccgt gcaagagcgg atctgctgca tgagtcaccc
     3721 agacagtcat tccgatgacg agcgccgccg cacgctggca gacgagctga tcgaggccgg
     3781 acgcttcttc ttcgcgcgcg gctgggtgcc ggcgaccagc ggcaacttct cggcgcgcgc
     3841 cggtgacgac gacgtgctga tcacggtttc gggccggcac aagggccggc tcggcaccga
     3901 cgacctgatg cacatcgacc tgcacggccg cccggcccgg gccggtcagc gttcctcggc
     3961 cgaaaccggc ctgcacctgc agctgtaccg gcgcgatccg gccatcggcg ctgtgctgca
     4021 cacgcactcg gtcaacgcga cggtgctgtc gatgctcgcc ggcggggcac tggtgctgcg
     4081 cgatctcgaa gtgctgaagg ctttcccggg catcgccacg cacgaaagcg ccgtgtgcgt
     4141 gccggtgctc gccaacgatc aggacatcgc gcgcctcgcc gcgcgcgtcg ccgaagtgct
     4201 cgatcgtgat ccgggctgct gcggctatct gatcgccggc cacggcttct acacctgggg
     4261 cgcgacggtc gccgacgcgg tacgccacgt cgaggccttc gagttcctgt tcgaatgcga
     4321 atccctgatg cggaggatgg gcagaccatg agcaagctgc gtgtttaccg ggacgacgcc
     4381 ccggacaccc cgatcgtcga acacacgctc ggcgccgaca tcgcgctgga gctcgaaggc
     4441 ccggaggtgc tgttctcgcg ctgggaggcc gaccgtgaac tgccggccga tgccagcaac
     4501 gacgacatca tcggcgccta ccgcgcgcag atcgacgtgc tgatgacgcg ctacggcttc
     4561 cacgccgtcg atgtcgtcaa cgtcacgccg acgcacccgg acaaggacgc gctgcgccag
     4621 aagttcctga gcgagcacac gcactcggac tacgaggtgc gcttcttcgt cgagggcagc
     4681 ggccagttct acatccacca cgccgatcgc gtgtacatga tcttctgcca ggccggcgac
     4741 ctgctgagca tcccggcgaa catgacgcac tggttcgaca tggggccgaa cccgagcttc
     4801 aagtgcatcc ggctgttcac gacgcccgag ggctgggtcg cgaacttcac cggcagcgac
     4861 atcgccgagc acttcccgcg gctggtcaac tgagccggca cggcagcgca tgtccgtggc
     4921 gatccgcgcg atcctgaccg acatcgaagg cacgaccagc tcgatcgcct tcgtgcacga
     4981 ggtgctgttc ccgtatgcgc gcgcgcggct gcccgcctgg ctggccgcgc acgccggcga
     5041 gccggaagtc gccggacagc tcgccgcgac ccgtgccgag gccggtctgc cgccggaggc
     5101 gccgcttgcg caggtcatcg aagtgctgct cggctggatc gacgccgatc gcaaggccac
     5161 gccgctgaag gcgctgcagg gccggctgtg ggccgacggc tacgcggccg gcgatttcca
     5221 cggccacgtc tatgccgacg tgccgccctg cctgcgcgcc tggcaggcgg ccggtctcgt
     5281 gctcgcggtg tattcgtccg gctcggtgca ggcgcagcag cagctgttcg cgcataccgc
     5341 ctgcggtgac ctgacgccgc ttttttcggc gtacttcgac acgcgcatcg gccacaagcg
     5401 cgagccggcg tcctacacgg cgatctgcgc cgcgctcggc ctcgcgccgg ccggggtgct
     5461 gttcctgtcc gacatcaccg aagaactcga cgccgcgcgc agcgcagggc ttgcgaccgt
     5521 gcagctcgtg cgcgacggca cacccgatcc gcaggccgcg caccggcagg tcaccgcctt
     5581 caccgccatc gacccgtacg cctgagccgc cgccggcgct tgtcgtgcgc cggcgaccgg
     5641 ctgctatgct cgggcgcaac gcgatcgtca ccgatccgtc gcccgcacgt tcacgctcac
     5701 gccccgccag acgagacctg atgactcccg agactcccga accggtcccg gcgctggaaa
     5761 tcgtcgacct gcacaaatgc ttcggctcgc tcgaagtgct gaagggcatc tcgctgagcg
     5821 cccgcgacgg tgacgtgatc tcggtgctcg ggtcctccgg ctccggcaag agcacctgcc
     5881 tgcgctgcat caacctgctc gaacagccga cctccggcac gatccgggtc aagggcgaga
     5941 cgctgaagct cgcgcgcgac cgcggcggcc agctcgccag caccgacccg aagcagctcg
     6001 cgcgcatccg ctcgcggctg gcgatggtgt tccagagctt caacctgtgg gcgcacatga
     6061 ccgtgctcga caacctgatc gaggcgccgg tgcacgtgct caagctcggg cgcgccgagg
     6121 cgatcgagcg cgccgagcgc ctgctgcaca aggtcggcct gtacgagcgg cgcaactact
     6181 atccggcgca cctctccggc ggccagcagc agcgcgcggc gatcgcgcgg gcgctggcga
     6241 tggagcccga tgccatcctg ttcgacgaac cgacctcggc gctcgatccc gagctggtcg
     6301 gcgaggtgct gaaggtcatg cgcgtgctcg ccgaggaagg ccgcacgatg atcgtcgtca
     6361 cgcacgagat gggcttcgcg cgcgaggtct ccagccgcgt cgtgttcctg caccagggcc
     6421 gggtcgagga agacggcccg cccgccgagg tgttcggcca cccccgttcc gagcgctgcc
     6481 ggcagttcct gtccggcaag ctcaagggct gacgccggtc cggccgccag agcgtccgcc
     6541 cgcccgtccg catcgtcacg ttcatccccg gaggcttgcg taccaacatg aagaagatcg
     6601 ccctcgctgc gctcggctgc gtgctcgcac tcggtatcgg tttcggcagc gtcgccgacg
     6661 cgaaggaatg gaagaaggtg cgcatcgcca ccgagggcgc ctatcccccg ttcaacttcg
     6721 tcgatgccga cggcaagctc gccggcttcg agatcgacct cgccaacgcg ctgtgcgccg
     6781 agatgaaggt cgaatgcacg ctggtcaagc aggactggga cggcatgatc ccggcgctga
     6841 aggcgcgcaa gtacgacctg atcatggcct cgatgtcgat caccgacgag cgcaagcagc
     6901 aggtcgattt ctccgactgg tactaccgga tgccggcgcg cttcgtcgcc aagaagggcg
     6961 ccggcctgac cgtcaccccc gaagggctca agggcaagac catcggcgtg cagcgcgaga
     7021 cggtctccga caagttcctg accgacaact acggatcgag cgtgacgatc aagcgctacg
     7081 cgacccagga cgaggtctac ctcgacctga ccgccggccg gctcgatgcc tacgtcgccg
     7141 acatcatcgt cagccgcgat gccttcctgt ccaagcccga aggcaaggac tacgagttca
     7201 ccggccccga gttcagcgat ccgaagtggt tcggcgacgg catcggcgcg gcgatgcgca
     7261 agcaggacaa cgacctgacg gcgctgttca acaaggcgct gaaggcgctg atcgccaacg
     7321 gcacctacca gaaggtcgcg gcgaagtacc cggacttcga ttcgtatctg cgcaagtgag
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccattccacc cggacgcttg
     7501 ccgatgctcg atttccacgg atacggcgcc tcggtgttcg aaggcgcatg gctgacgctc
     7561 gaagtggcgc tgatgtcgct ggtggtcgcc gtcgcgctcg gccttgccgg tgcgctggcg
     7621 aagctgtcgc gctcgctggt gctgcgcacg atcgccgggc tgtacacgac catcatccgc
     7681 ggcatgcccg atctggtgct gatgctgctg atctacttcg gcggccagac gctggtgaac
     7741 gtcatcagcg atgcgctcgg ctgggagtac atcgacatca atcccttcgt cgccggcgtg
     7801 ctgaccatcg gcctgatctt cggcgcctac atgaccgaga ccttccgcgg cgcgctgatg
     7861 gcggtgccga agggccagct cgaagccggc tacgcctacg gcatgagcgc cacgcaggtg
     7921 ttcgtgcgca tccacctgcc gcagatgctg cgctacgcac tgccgggctt cggcaacaac
     7981 tggctggtgc tgctgaagac cacggcgctg gtgtcggtga tcggcctcga tgacatcgtg
     8041 cgcaaggccc agctcgccgc cggcgccacg cgcctgccgt tcacgttcta cggcgccgtg
     8101 ctgctcggtt ttctggcgtt cacgaccgtc tcggtgctgc tgctgcgctg ggccgaacgc
     8161 cgctacagcc tcggctacga gcggatggag tgagcggcga tggaattcgg cgtcatcctc
     8221 gacaactggc cgctgtacct cgaaggcctg gtcacgacgg tcgagctgac cggtctgtgc
     8281 ctgatcctcg gcctgctgct cgcgatcccg ctcggcaccg tgcaggcggt cgggccgctg
     8341 tgggcgcgcg gcctggtgtt cgcgtacagc tacgtgttcc gcggcacgcc gctgctggtg
     8401 cagaccttcc tgatctacta cggcctcggc cagttcgagg ccgtgcagca gagcgtgttc
     8461 tggccgctgc tgaaggaagc ctactggtgt gcgctgatcg cgttcacgct caacaccggc
     8521 gcctacacca ccgagatcat ccgcggctcg atcgtgacca tgccgcgcgg cgagatcgag
     8581 gccgcgcgcg cctgcggcat ggacaccttg ctgatgctgc ggcgcatcgt gctgccgagc
     8641 gccttccgcc gcgcgctgcc ggcctacggc aacgaggtca tcttcatgct gcagggctcg
     8701 tcgatcgcct cgacgatcac gctggtggac ctgaccgggg ccgcgcgcgt gatcaactcg
     8761 cgctactacc tgccgtatga ggctttcctg accgccggcc tgctgtacat ggcgctgtcc
     8821 ttcctgctgt tcgcgggctt ccgcaagctc gaacaacgct ggttcgcgca cctgcggccg
     8881 cgggagcatt gaggccccgc aaccgtgaac accgggtctg gcgcccgctg cgcgcgccgg
     8941 ccggccccgc ttcagtccag cagcatctcc actgcggccg caaagtcatc ggcgagcccc
     9001 gcccgcggac acttgcgctc gtcgccatcc cggtacttgc cggtgcgtac cagcactgcg
     9061 gcgccgaggc cggcggcgag cgcggcggcg acatcggcct ccgcatcgtc gccgatcatc
     9121 gccacacgct gcggcggcag gccgagccgc gcgcagccgg tctcgaacat cgcctgcgcc
     9181 ggcttgccga tgatggtcgc gctgacgccg ctcgcgtatt cgagcgcggc gcggaacggg
     9241 ccgatgtcga gcgcgagccg ctcgccgtcg cggaagtagc gattgtcggc catcgccacc
     9301 agttgcgcgc cgccgagcag cagctgcagc gcatggttca gattctcgta gtggaagccg
     9361 tcggcggcat cgccgagcac gacgacatcc ggcgactcct gttcgagatc ggcaaaagcc
     9421 gcgatcacgg ccggatgcac gagcagatgc ggacgcaggc ggcgcacggc cagccagtcg
     9481 tgcgtggcat cgacggcgct gacgatgcgc gattccggca ccggcaggcc gagcccggcg
     9541 agctgggcgg cgatcgcggt gcgcgtctgg cgcgtggtgt tggtgacgaa ggcatgcggc
     9601 acgccggccg cggcgaggcg cgccagcgtt tcggccgctc ccggcagcgc gcggtcgttg
     9661 acgtgcagca cgccggccag atcgatcagc agcccctcgg gtttcgcggc aacagcggtc
     9721 atctgcaggc tccggtcggc gggctgcgca ccagcataac gctttgctgc gctgcgcaaa
     9781 gcccgccggc gactagctga cggcttgcgg cgcacccgtg ggcgagggcg gtgcttgcgt
     9841 ataatcgccg ctctgtttcc gcgcccaccc ggggaatccg caccgtcatg cagtacctca
     9901 gcacgcgcgg cgacgccgcg gcccgcacgt tttccgacat cctgctcgcc ggcctggcgc
     9961 cggacggcgg cctctacctg cccgagcact atccgcaggt gagcgcggcc gaactcgccg
    10021 cctggcgcga gctgccgtat gcggaactgg ccttcgaaat cctgcgcaag ttcgcgaccg
    10081 acctcccgga agccgacctg cgcgcactga ccgcgaagac ctacaccgcc gccgtctacc
    10141 gcagcgacgc gatcacgccg ctgcgcacgc tcgaacccgg cctgtacatc ctcggtctct
    10201 cgaacggccc gacgctggcg ttcaaggaca tggcgatgca gctgctcggg aatctcttcg
    10261 agtacgtgct cggacagagc ggcgaggagc tgaacatcct cggcgccacc agcggcgaca
    10321 ctggatcgag cgccgagtac gcgatgcgcg gcaagcgcgg catccgcgtg ttcatgctgt
    10381 cgccgcacgg caagatgagc gccttccaga ccgcgcagat gttcagcctg ctcgacccga
    10441 acatccacaa cctcgccgtg cgcggcgtgt tcgacgactg tcaggacatc gtcaaggccg
    10501 tctccaacga tcacgccttc aaggcgcgcc atcgcatcgg cacggtcaac tcgatcaact
    10561 gggcgcgcgt ggtcgcgcag gtcgtgtact acttccgcgg ttacttcgcg gcgacccgca
    10621 gcaacgacga ggaagtcgcg ttcgcggtgc cgagcggcaa cttcggcaac atctgcgccg
    10681 gccacatcgc gcggcagatg ggcctgccga tccgccggct ggtgctcgcg accaacgaga
    10741 acgatgtgct cgacgagttc ttccgcagcg gcgtctaccg cccgcgcgcc agtgccgaga
    10801 cctggcacac gtcgagtccg tcgatggaca tctccaaggc ctcgaacttc gagcgcttcg
    10861 tgttcgacct gatgggccgc gatgccgcga aggtgcgcga actctgggcg cgggtcgacg
    10921 ccggcggcgc cttcgatctg tccggcacgc cggaattcgc cgcgatcacc gggcgctacg
    10981 gcttcgtctc cggcagcagc acgcacgccg accgcctcgc caccatccgc gacaccgccg
    11041 cgcgcttcga cgacgtgatc gacacgcaca ccgccgacgg cctcaaggtc gcgcgcgaat
    11101 tcaccgaacc cggcgtgccg atgatcgtgc tcgaaaccgc gctggcggcg aagttcgagg
    11161 aaaccatcat cgaagccctc ggccgcaagc ccgggcgccc ggacggctac gaacggctcg
    11221 aatccctgcc gcagcgcttc gaggtcatcg acgccgatgc cgcgcaggtg aaggcgtaca
    11281 tcgcgaggca tgtcggcggc tgatgccgcc ctcacggccg gcggagcagc catccgccgg
    11341 ccgaccgtgc tagcatccgg ctcccgcctg cccacacccc gccccggtag ctcagctgga
    11401 tagagcgtcc ccctcctaag gggaaggtcg gaggttcgaa tcctctccgg ggcacca
//
LOCUS       DAOQIE010000096        11435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2811_length_11435_cov_6.719156, whole genome shotgun sequence.
ACCESSION   DAOQIE010000096 DAOQIE010000000
VERSION     DAOQIE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2811_length_11435_cov_6.719156"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..405)
                     /gene="iscA"
                     /locus_tag="PK144_07690"
     CDS             complement(82..405)
                     /gene="iscA"
                     /locus_tag="PK144_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNF65648.1"
                     /translation="MAITLTEAAAKRAQTYIDKHEHGLGLRLGVRSSGCSGFAYVVKV
                     ADAIHADDQVFEDRGLKLVVDRKSLPFIDGTELDFVREGLNEAFRFTNPNVKSQCGCG
                     ESFAV"
     gene            complement(421..831)
                     /locus_tag="PK144_07695"
     CDS             complement(421..831)
                     /locus_tag="PK144_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNF65649.1"
                     /translation="MNYGPAAQIHFRDPQHAGTFACGTPGVSRATAGPSADGDLIRLE
                     LRFGRDGRIAEVRQSACGTVAAIAAASWLATQLPGLDAGTARALHHADLAAALGLPAQ
                     RLPASVLALRALAAALDAAAGRLPETGSDLRSTA"
     gene            complement(889..2043)
                     /locus_tag="PK144_07700"
                     /pseudo
     CDS             complement(889..2043)
                     /locus_tag="PK144_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2066..2545)
                     /locus_tag="PK144_07705"
     CDS             complement(2066..2545)
                     /locus_tag="PK144_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF65650.1"
                     /translation="MKLTTKGRYAVTAMLDLALHFHGEPVVLSDISERQNLSLAYLEQ
                     LFAKLRKQGLVASTRGPGGGYSLARSADAITLAEVIEAVDESVDATRCGGLGNCHGDH
                     QCLTHELWVELSSHIHAFLQGRTLGEMARRIGTRQPAVSTHPLRHFVQHDLRRAAGD"
     gene            complement(2643..3422)
                     /locus_tag="PK144_07710"
     CDS             complement(2643..3422)
                     /locus_tag="PK144_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF65651.1"
                     /translation="MLPNLRVVLVETSHPGNIGAAARAMKNMGASELVLVAPERYPDP
                     AASARAAGADDVLTAARVVATLPEALAGCACAFGASARPRHIVAEVMDARTAASRIAP
                     MLTAGETCALVFGRERTGLTNAEMAYCTQFVHIPTHPGFSSLNVAAAVQVLCYEVRVA
                     LLALQESGEAPAPFDRLAAHDDMEQFYAHLEQVLIEIGFHDPQRPRHLMRRLRRLFAR
                     AAVEQNELAILRGVLSAVQGGKQMRRTDAQLTQISGEVPNS"
     gene            3548..4345
                     /locus_tag="PK144_07715"
     CDS             3548..4345
                     /locus_tag="PK144_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF65652.1"
                     /translation="MHPTLTIAQRAAQAAAKVILRNIDRLDQLTVSEKGRHDFVSEVD
                     VQAEREIVKVLRKTYPDHAILAEESGAAGESDYQWIIDPLDGTTNFLHGHPQFAISIA
                     LKVRGRLESALIHDPLRQETYAAARGEGATLNDRRIRVKPAAGLDGALLGTGFPFRAP
                     RHMPAYLACFAALAEHCGEMRRAGSAALDLAYVAAGRLDGFWEIGLAEWDMAAGVLLV
                     QEAGGLVGDFGGGAEYFRTGNVVAGTPKVFKGLLQTIQPHLTPALSR"
     gene            4608..4952
                     /locus_tag="PK144_07720"
     CDS             4608..4952
                     /locus_tag="PK144_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65653.1"
                     /translation="MQKKTLAFLAPPFAIARFGGRVAWCAAPIGTVWMSAIVGILIGI
                     QGDMSTATREPMLWVIALGLAIWAVSSVWALMVIGGVDHDVAHDADSTLEHTVHAEED
                     ELDPLEQIRGMR"
     gene            complement(5108..6040)
                     /gene="secF"
                     /locus_tag="PK144_07725"
     CDS             complement(5108..6040)
                     /gene="secF"
                     /locus_tag="PK144_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNF65654.1"
                     /translation="MQLIPHTLDIRFMRFGRMSLILTLLLMLVCAGALVFKGLNYGLD
                     FTGGTVIEVRYAQAIEMPQVRSALAAGGFADAQTQYIGSAAEVLIRVPPHEGSDAAAL
                     STHILDALKLGNDGAVEVRRVEFVGPQVGGELVQNGSLAAVFAMLGILVYVAFRFEWR
                     LALGAIAATLHDVVFCIGVYALFGIEFDLTGLAAILAVIGYSVNDTVVVFDRIRENFR
                     RLRKDTPERVIDISINETLARTIMTSMTTLLAVVVLYVWGGDAIHTFSLIMIVGVLIG
                     TYSSIYVASATALHLGLSREDLLAKVKEEGDARP"
     gene            complement(6054..7907)
                     /gene="secD"
                     /locus_tag="PK144_07730"
     CDS             complement(6054..7907)
                     /gene="secD"
                     /locus_tag="PK144_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNF65655.1"
                     /translation="MNQFAWWKNLLIVLVCVIGALYALPNVFGEDPAVQISATRAAPV
                     DQNLIERVGSELQGLKIQPKTFELENGRLLVRLTGTDDQLRAADRLGQALGNDYIVAL
                     NLAPATPQWLRNLRASPMFLGLDLRGGVHFLMEVDMKAVVKQLEERYQEEIRTLLRTE
                     KIRYNAVGRAQGGGVKVSFRDGTDAQKGLGVIRDKLPALVPGPASSDGTELSFQFSEK
                     ELADKQTFALQQNITTLRNRVNELGVAEPIIQQQGDARIVVQLPGVQDTARAKEILGA
                     TATLEFRLVDETGDVAAALNGQVPVNDRLYRERNGRPLLLERRVILTGDYITDASSGI
                     EQQTGGSAVYINLDGKGARRMEDATKDAIGRMMAVVFIENKTETKTVDGKAQKVSTTV
                     EEVINVARIRDRLGARFQITGLDNPQEARNLALLLRAGALAAPIQIVEERTVGPSAGR
                     ENIEQGFDSSIAGFVVVAIFMIVSYRLCGLISVVALAVNVILIIAALSLLQATLTLPG
                     IAGIVLTMGMAVDANVLIYERLREELRLGLTPQAAIKAGFERAFATILDANVTHLIAG
                     IALFSLGSGPVKGFAVTLIIGILTSMFTAVTVSRALVNLVYGGRKVGRISV"
     gene            complement(8040..8372)
                     /gene="yajC"
                     /locus_tag="PK144_07735"
     CDS             complement(8040..8372)
                     /gene="yajC"
                     /locus_tag="PK144_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNF65656.1"
                     /translation="MSFLISDAYAQAAPAGAASDPIMQFLPLVVIMVAFYFILLRPQQ
                     KRQKEHKSMIEALKKGDEVVTGGGVLGRIVEVGDNFVSLEVADKVEIKVQKAAVSSLL
                     PKGTVKGL"
     gene            complement(8508..9635)
                     /gene="tgt"
                     /locus_tag="PK144_07740"
     CDS             complement(8508..9635)
                     /gene="tgt"
                     /locus_tag="PK144_07740"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNF65657.1"
                     /translation="MEFQLFTTDGAARRGRMTFARGTVETPAFMPVGTYGTVKAMTPE
                     EIREIGAEIILGNTFHLMLQPGTDVIGAHGDLHDFMHWSGPILTDSGGFQVFSLAELR
                     KISEQGVTFRSPVNGDKVFLGPEESMQVQRALGSDIVMIFDECTPYPATETEARKSME
                     LSLRWAKRSRAEHDRLANPNALFGIVQGGMYPVLRTQSAMGLAEIGFDGYAVGGLAVG
                     EPMAERNHILEHTVPQLPVERPRYLMGVGKPEDIVEAVARGIDMFDCVMPTRNARNGH
                     YFTWDGDVRIRNSRHRLDTGPISHDCDCYACRHYSRAYLKHLDRNQEILGARLATIHN
                     LHFYQQLMARIRAAISEGCFEEFRAGFHARRGGGAAADGCV"
     gene            complement(10218..10691)
                     /locus_tag="PK144_07745"
     CDS             complement(10218..10691)
                     /locus_tag="PK144_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF65658.1"
                     /translation="MKSSVYIETSVLSYLTARPSNDLRAMANQSVTAEWWETQKDHYS
                     LVASELVVSEAGCGHPEAAQRRLAVVNSLPLLQVSQQAQELAQALIQEYALPKKAEVD
                     ALHVALAAVNGVEYLLTWNCTHIANAHTRPKIEATCRAHGYEPPVICTPLELMEN"
     gene            complement(10791..>11435)
                     /locus_tag="PK144_07750"
     CDS             complement(10791..>11435)
                     /locus_tag="PK144_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HNF65659.1"
                     /translation="EGDEKQRLQALQHIDPSFAEIFFGSYPVLVEGDTEHAAFMAAVI
                     KKQHELLNSVTVIRARGKAILAPLVKVMEHFKIDFGIVHDCDPPFKKNGDNNGMWTEN
                     KKIRDAIVKARTSGVTVRHRVSVPDFERFLGGEEESKDKPFTAYRRITNEAALSEIVQ
                     KLLGELLSGDQHEPFDRETFPEGSDYIEVLSQKIGSWAQENGLSDEIRFKGKPT"
BASE COUNT         1807 a         3832 c         3888 g         1908 t
ORIGIN      
        1 gccggtcggc gcgcgggcgg caagtgtacc ggctgcaccg ggcagcggca acgcgcgtcc
       61 cggtgccgcc cggacctgac ttcagaccgc gaaactctcg ccgcagccgc actgcgactt
      121 gacgttcggg ttcgtgaacc ggaacgcctc gttcaggccc tcgcgcacga agtcgagttc
      181 ggtgccgtcg atgaacggca ggctcttgcg atcgacgacg agcttcaggc cccggtcctc
      241 gaacacctgg tcgtcggcat ggatggcatc ggcgaccttg accacgtagg cgaagcccga
      301 gcagcccgag ctgcgcacgc cgaggcgcag gccgagcccg tgctcgtgct tgtcgatgta
      361 ggtctgcgcg cgcttcgccg cggcttcggt cagcgtgatg gccatggtgg gactcctcgt
      421 tcaggcggtg gatcggaggt cggaaccggt ttcgggcagc cggccggcgg cggcgtcgag
      481 tgccgccgcg agggcgcgca gcgccagcac cgacgccggc aggcgctgcg ccggcaggcc
      541 gagggcggca gcaagatcag catgatgcag cgcacgggct gtgcccgcgt cgagcccggg
      601 cagctgcgtg gccagccagg atgcggcggc gatcgccgcg accgtgccgc aggccgactg
      661 gcggacttca gcgatgcgcc cgtcgcgccc gaagcgcagt tcgaggcgga tcaggtcgcc
      721 gtcggccgac ggaccggccg tggcacggct cacgcccggc gtgccgcagg cgaacgtgcc
      781 ggcatgctgc ggatcgcgga aatgaatctg ggcggcgggg ccgtaattca aggcggccag
      841 ggcttcggga tcggtgccgg cggcttcggc gagcggtgca tcggggcaga gcggcgacag
      901 tccgcgcagc cagcccacgg cccgcgtgat ccgggcgatg gccagatcga tgtcggcgag
      961 gctcgtcgat cgccccggcg acagccgcag gctgcagcgg gcgggggcat ccggcacgcc
     1021 cagcgcgcgc agcacgtgcg agggctgcgc ctcggccgag ctgcaggccg agccgcccga
     1081 cagcgcaagc cccggcagcg cggcgaacag gctctcgctg ccgatgccct cgaagccgag
     1141 gttcaggatg ccggcgacgc gctgctgcgg atgcccgtgc aggtgcgtgc cgggcaggtg
     1201 acagagcccg gcccgcagcc gttcatcgag cgccgcgacg tgccgcgcgt cggcctcgcg
     1261 ccgctcgcgc agcagccggc aggccgtgcc gaagccgacg atctggtgca cggccagcgt
     1321 gccggcgcgc aggccgcgct cctgaccgcc gccgtgcatc tgcgcatcga gccgcacgcg
     1381 cggcagccgg cgcacgtaca gcgcgccgat gcccttcggt ccgtacagct tgtgcgccga
     1441 cagactcatc aggtcgagct cgggcagcca tgccgccaga tcgatgcgcc cggccgcctg
     1501 cgcggcatcg acgtggaaca gcgcgccgtg cgcacgcacg agccgcccga gcgcggcgat
     1561 gtcctgcacg acgccggtct cgttgttgac ggccatcacc gacaccagcg cggtgtccgg
     1621 gcgcagcgcc gcagcgagtc gcgccggatc gagcaggccg tcggcgccgg gatcgagcac
     1681 ggtcagcccg gcgccggcgc ggcgcagctg cgcgcagaca tcgagcaccg acttgtgttc
     1741 ggtcgaacag gtgatcaggt gccggccgcg gcggctggcc tgcagcgcgc ccttcagcgc
     1801 cagattgctg gcttcggtgg ccccggaggt ccagaccagc tcggccggat cgcagccgag
     1861 cagcgccgcc agttcggcgc gtgcctgccc gacgcgcgcg gcagcccgcc ggccatgggc
     1921 gtgggtggtc gaggccggat tgccgaagtc cccgtcggcg cccagacatt cgagcatcgc
     1981 ggcaacgacc tgcgcatcga ccggcgtggt cgcggcgtga tcgagataga cggcgctgct
     2041 catcgcatcg gtgcgcgcac cggcctcagt cgccggcggc gcggcgcagg tcgtgctgca
     2101 cgaagtgacg cagcggatgc gtgctgacgg ccggctggcg ggtgccgatg cgccgcgcca
     2161 tctcgccgag cgtgcggccc tgcaggaagg cgtggatgtg gctggagagt tcgacccaga
     2221 gctcgtgggt caggcactgg tgatcgccgt gacagttgcc gaggccgccg cagcgcgtcg
     2281 catcgaccga ctcgtcgacg gcctcgatga cttcggcgag cgtgatcgca tcggcgctgc
     2341 gggccagcga atagccgccg ccggggccgc gcgtgctcgc gaccaggccc tgcttgcgca
     2401 gcttcgcgaa cagttgttcg aggtaggcga gcgacaggtt ctgacgttcc gagatgtccg
     2461 acagcaccac cggttcgccg tggaaatgca gcgcgagatc cagcatcgcg gttaccgcat
     2521 agcggccttt cgtcgtcagt ttcatgtctg cccccgtcgc ttccgcctcg atagtccggc
     2581 cccgtgcgta gcaggaccgt ctttagtgtg ggccgcttta tactcgtacc cgagaaaagc
     2641 ggtcaactat taggcacttc tccgctgatc tgcgtcagct gtgcatcggt gcggcgcatc
     2701 tgcttcccgc cctgcaccgc cgacagcacg ccgcgcagga tcgccagttc gttctgctcg
     2761 accgcggcgc gcgcgaacag ccggcgcagc cggcgcatca ggtggcgcgg gcgctgcgga
     2821 tcgtggaagc cgatctcgat cagcacctgc tccaggtgcg cgtagaactg ctccatgtcg
     2881 tcgtgcgccg ccagccggtc gaacggtgcc ggcgcctcgc cgctctcctg cagcgccagc
     2941 agcgcgacgc gcacctcgta gcagagtacc tgcacggcgg cggcgacatt cagcgagctg
     3001 aatccgggat gggtcgggat gtgcacgaac tgcgtgcagt aggccatctc ggcattggtc
     3061 agcccggtgc gctcgcgccc gaacaccagc gcgcaggtct cgccggccgt cagcatcggc
     3121 gcgatgcggc tcgcggcggt gcgcgcatcc atgacctcgg cgacgatgtg ccgcggacgc
     3181 gccgaggcgc cgaaggcgca ggcgcagccg gcgagcgcct cgggcagcgt cgcgaccacg
     3241 cgcgccgccg tgagcacgtc atcggcaccg gcggcgcgcg cactggcggc cgggtccgga
     3301 tagcgttccg gcgccaccag caccagctcg ctcgcgccca tgttcttcat cgcgcgcgca
     3361 gcggcgccga tgttgccggg atgactggtt tcgacgagga cgacgcggag gttcggcagc
     3421 agcatgcgtg caggcggtcc ggtacggcgc gggggcggat cggatatgct acgcgattct
     3481 ttgcccgacc cgggctccga tctttcccaa gaccgctcca gagcgccccc gaagcgaagc
     3541 cgccgccatg catccgaccc tcaccatcgc ccagcgcgcc gcccaggccg ccgccaaggt
     3601 catcctgcgc aacatcgacc ggctcgatca gctgacggtc agcgagaagg ggcggcacga
     3661 cttcgtcagc gaggtcgacg tgcaggccga gcgcgagatc gtcaaggtgc tgcgcaagac
     3721 ctaccccgat cacgcgatcc tcgccgagga gagcggcgcg gccggcgaat ccgattacca
     3781 gtggatcatc gatccgctcg acggcacgac caacttcctg cacggccacc cgcagttcgc
     3841 gatctcgatc gcgctgaagg tgcgcgggcg cctcgaatcc gcgctgatcc acgatccgct
     3901 gcgtcaggaa acctacgccg ccgcgcgcgg cgagggcgcg acgctcaatg accgccgcat
     3961 ccgcgtcaag cccgcggccg gcctcgacgg cgcgctgctc ggcaccggct tcccgttccg
     4021 ggcgccgcgg cacatgccgg cgtatctggc ctgcttcgcg gcgctggccg agcactgcgg
     4081 cgagatgcgc cgcgccggct cggcggcgct cgatctcgcc tacgtcgccg ccggccgcct
     4141 cgacggcttc tgggagatcg gtctggccga atgggacatg gccgccggcg tgctgctggt
     4201 gcaggaagcc ggcggcctgg tcggtgactt cggcggcggc gccgagtact tccgcaccgg
     4261 caacgtcgtc gccggcacgc ccaaggtatt caagggcctg ctgcagacca tccagccgca
     4321 cctgacgccg gcactgagcc gctgaatccc cccgcctccg gatctctgct gcggcgcgcc
     4381 ataaattacc ggatgccatg atggcaacga ctttcgttgc cgtcatgcgc aagagcctgc
     4441 acggccgcat cggactgctc tagtctgacc ccggtttgca cccggccgaa ggctgtctgc
     4501 cacggcagcg aacttcggga ctgaccgcgc actccgacgt accgccacag ttcccgcccc
     4561 cataagcaag cgcggcatac ccgatcacgg ggattcagga ggtcagcatg cagaagaaaa
     4621 ccctcgcctt tctcgcgccg ccgtttgcca tcgcccgctt cggcggccgc gtcgcctggt
     4681 gcgcagctcc gatcggcacg gtctggatga gcgcgatcgt cggcatcctg atcggcatcc
     4741 agggcgacat gagcacggcc acccgcgaac cgatgctgtg ggtcatcgcc ctcggcctgg
     4801 cgatctgggc cgtgtcctcg gtgtgggcgc tgatggtcat cggcggcgtc gatcacgatg
     4861 tcgcgcacga tgccgacagc acgctcgaac acacggtgca cgccgaggaa gacgaactcg
     4921 acccgctcga acagatccgc ggcatgcgct gaacgcgccg cgcggccgtc gcaaggcggc
     4981 cggaaaacac aaaggggagg ccggccggcc tccccttttt catgccccct tctctcccca
     5041 tctcactccc gcgagggccg aggggctgaa aagcgaagca ttcctgatga tggcggtgtg
     5101 cccggcctca cggccgcgcg tcgccctcct ccttcacctt cgccagcaga tcctcgcgtg
     5161 acaggccgag gtgcagtgcg gttgccgagg cgacgtagat cgacgagtac gttccgatca
     5221 gcacgccgac gatcatgatc agcgagaagg tgtggatcgc gtcgccgccc cagacgtaga
     5281 gcacgaccac cgcgagcagc gtcgtcatgc tggtcatgat cgtgcgggcc agcgtctcgt
     5341 tgatcgagat gtcgatgacg cgctccggcg tgtccttgcg cagccggcgg aagttctcgc
     5401 ggatgcggtc gaacacgacg acggtgtcgt tcaccgagta gccgatcacg gcgaggatcg
     5461 cggcgaggcc ggtcaggtcg aactcgatgc cgaacagcgc gtagacgccg atgcagaaca
     5521 ccacgtcgtg cagggtcgcg gcgatcgcgc cgagcgcgag ccgccattcg aagcggaagg
     5581 cgacgtagac gaggatgccg agcatcgcga acaccgccgc cagcgaaccg ttctgcacca
     5641 gctcgccgcc gacctgcgga ccgacgaact cgacgcggcg cacctcgacc gcaccgtcgt
     5701 taccgagctt cagcgcatcg aggatgtgcg tgctgagcgc cgcggcgtcg ctgccctcgt
     5761 gcggcggcac gcggatcagc acctcggcgg ccgagccgat gtactgggtc tgcgcatcgg
     5821 cgaagccgcc ggcggcgagc gccgagcgca cctgcggcat ctcgatggcc tgcgcgtagc
     5881 gcacctcgat gacggtgccg ccggtgaagt cgagcccgta gttgaggccc ttgaacacca
     5941 gcgcgccggc gcagaccagc atcagcagca gggtcaggat cagcgacatg cgcccgaagc
     6001 gcatgaagcg gatgtcgagc gtgtggggaa tcagctgcat ggcacgcgct ccgttacacc
     6061 gagatgcggc cgaccttgcg accgccgtag acgaggttga ccagtgcgcg gctgacggtg
     6121 acggccgtga acatcgaggt caggatgccg atgatcagcg tcaccgcgaa gcccttgacc
     6181 gggccggagc cgagcgagaa cagcgcgatg ccggcgatca ggtgcgtgac gttggcgtcg
     6241 aggatggtcg cgaacgcccg ctcgaagccg gccttgatcg ccgcctgcgg cgtcaggccc
     6301 aggcgcagct cctcgcgcag gcgctcgtag atcagcacgt tggcgtcgac cgccatgccc
     6361 atcgtcagca cgatgccggc gatgccgggc agcgtcagcg tcgcctgcag cagcgacagc
     6421 gccgcgatga tcaggatgac gttgaccgcc agcgccacca ccgagatgag gccgcagagc
     6481 cggtacgaga cgatcatgaa gatcgcgacg acgacgaagc cggcgatcga gctgtcgaaa
     6541 ccctgctcga tgttctcgcg tccggcgctc gggccgaccg tgcgttcctc gacgatctgg
     6601 atcggcgcgg ccagcgcgcc ggcgcgcagc agcagcgcga ggttgcgcgc ttcctgcggg
     6661 ttgtcgaggc cggtgatctg gaagcgcgcg ccgaggcggt cgcggatgcg cgcgacgttg
     6721 atgacttcct cgaccgtggt cgagaccttc tgcgccttgc cgtcgacggt cttcgtttcg
     6781 gttttgttct cgatgaacac caccgccatc atccggccga tcgcgtcctt ggtcgcatct
     6841 tccatgcgcc gcgcgccctt gccgtcgagg ttgatgtaga ccgccgaacc gccggtctgc
     6901 tgttcgatgc cggagctggc gtcggtgatg tagtcgccgg tcaggatcac gcgccgttcg
     6961 agcagcagcg gccggccgtt gcgctcgcgg tacaggcggt cgttgaccgg cacctggccg
     7021 ttgagcgccg ccgccacgtc gccggtctcg tcgacgaggc ggaattcgag cgtcgccgtg
     7081 gcgccgagga tttccttggc gcgcgccgtg tcctgcacgc ccggcagctg cacgacgatg
     7141 cgcgcatcgc cctgctgctg gatgatcggc tcggccacgc ccagctcgtt gacgcggttg
     7201 cgcagcgtcg tgatgttctg ctgcagcgcg aaggtctgct tgtcggcgag ttccttctcc
     7261 gagaactgga agctcagctc cgtgccatcc gacgaagccg gccccggcac cagcgccggc
     7321 agcttgtcgc ggatcacgcc gaggcccttc tgcgcatcgg tgccgtcgcg gaagctgacc
     7381 ttcacgccgc cgccctgcgc acggccgacc gcgttgtagc ggatcttctc ggtgcgcagc
     7441 agcgtgcgga tttcctcctg atagcgctct tcgagctgct tcacgaccgc cttcatgtcc
     7501 acttccatca ggaagtgcac gccgccgcgc aggtcgaggc cgaggaacat cgggcttgcg
     7561 cgcaggttcc tgagccactg cggcgtggcc ggggccaggt tcagcgcgac gatgtagtcg
     7621 ttgccgagcg cctgcccgag ccggtcggcg gcgcgcagct gatcgtcggt gccggtcagg
     7681 cgcacgagca gacggccgtt ctcgagctcg aaggtcttcg gctggatctt cagcccctgc
     7741 agctcgctgc cgacgcgctc gatcaggttc tggtcgaccg gcgcggcgcg ggtcgccgag
     7801 atctgcacgg cggggtcttc accgaaaacg ttgggcaggg cgtacagcgc gccgatcacg
     7861 cacacgagca cgatcagcag gttcttccac caggcgaact ggttcatcga cgacggctcc
     7921 cggccggagc gcagcgcgca tccggaaaac gacccgaccg cgccggcggc cgcgccgtcg
     7981 tccggtcctg cggacgaagg cgggccgccg gcgcggtcgc gaggctggct ggctggggct
     8041 cagaggccct tgaccgtgcc cttgggcagc agcgagctga cggcagcctt ctgcaccttg
     8101 atctcgacct tgtcggcgac ttcgagcgac acgaagttgt cgccaacctc gacgatgcgc
     8161 ccgagcacac cgccgccggt gacgacctcg tcacccttct tcagcgcctc gatcatgctc
     8221 ttgtgctcct tctgccgctt ctgctgcgga cgcagcagga tgaagtagaa ggcgaccatg
     8281 atcacgacca gcggcaggaa ctgcatgatc ggatcgctgg cggcgccggc gggggcggcc
     8341 tgggcgtagg cgtcggaaat caggaagctc atggactcac tttctccgtg gggtcggttc
     8401 gttgtgctgg ccgctcgtcc ggtcgggggc ggctctggcg ccggcagctt gtgcctgccg
     8461 gcaccggtcg ccgcccgtcg ggacggcggc cggcaaaagg tcgcgcatta tacacagccg
     8521 tccgcagccg ccccgccgcc acggcgggcg tggaagccgg cgcggaattc ctcgaagcag
     8581 ccctcgctga tcgcggcgcg gatgcgtgcc atcagctgct ggtagaagtg caggttgtgg
     8641 atggtggcca ggcgcgcgcc gaggatttcc tgattgcggt cgaggtgctt cagatacgcg
     8701 cgcgagtagt gccggcaggc gtagcagtcg cagtcgtggc tgatcgggcc ggtgtcgagc
     8761 cggtggcggc tgttgcggat gcgcacgtcg ccgtcccagg tgaagtagtg gccgttgcgg
     8821 gcgttccgcg tcggcatcac gcagtcgaac atgtcgatgc cgcgcgccac cgcctcgacg
     8881 atgtcctcgg gcttgccgac gcccatcagg tagcgcggcc gctccaccgg cagttgcggc
     8941 acggtgtgtt cgaggatgtg gttgcgctcg gccatcggct cgccgaccgc gagcccgccg
     9001 accgcgtagc cgtcgaagcc gatctcggcc agccccatcg ccgactgcgt gcgcagcacc
     9061 ggatacatgc cgccctgcac gatgccgaac agcgcgttcg ggttggccag gcggtcgtgc
     9121 tcggcgcgcg atcgcttcgc ccagcgcagc gacagttcca tggacttgcg cgcttcggtc
     9181 tcggtcgccg ggtagggcgt gcattcgtcg aagatcatga cgatgtcgga gccgagcgcg
     9241 cgctgcacct gcatggattc ctcgggaccg aggaacacct tgtcgccgtt gaccggcgag
     9301 cggaaggtca cgccctgctc gctgatcttg cgcagctcgg cgagcgagaa cacctgaaag
     9361 ccgccggagt cggtcaggat cggcccgctc cagtgcatga agtcgtgcag gtcgccgtgg
     9421 gcgccgatca cgtcggtgcc gggctggagc atcaggtgga aggtgttgcc gaggatgatc
     9481 tcggcgccga tctcgcggat ttcctccggc gtcatggcct tgacggtgcc gtaggtgccg
     9541 accggcatga acgccggggt ttcgaccgta ccgcgcgcaa acgtcatccg cccgcgccgc
     9601 gcggcgccgt cggtcgtgaa caactggaat tccatgggga tgcggccggc cggtgaaggg
     9661 cgcgacagtt tcgtgaccgc aaccgggctt ggcaagccgg ttaccgcgga cggcgcggca
     9721 aggctgcaca cccttgcgtg ttgacggtcc gggctgatct tgcgggaccg tggccacggt
     9781 tcacggtccc cgacgcttcc ccgccgcgac cgaaggcgct gaaaagctcc cggacaagca
     9841 ccccggcagc acctgcggct ggcgaggcag gcgcaggggc atgagcaagg cagcgagcgc
     9901 aggggatgac ggtgattgcc gtgaatctgc aggcgatgaa cagaatcaac gaagatgttg
     9961 cggattcgtg cgcacgcgga ttttgaaagc ttgtacggcg tgcgcatgaa tgcgcaccct
    10021 acccggctgg agcgtcttgt tggggagcca ctgtgttgac cgccggttta cgcggaggga
    10081 aggacaccat gcgctcggga tgagccgctt gatgcgtcat gaggtcttta cagatagcct
    10141 caatgtcata gttgaactgg cgtgcatact cgtctctaag tgcgcgtgtt tctgaaacaa
    10201 cggggtcttg ccacatatca gttctccatg agttcaagcg gagtgcagat gactggtggt
    10261 tcgtagccgt gcgctctgca ggttgcttcg attttaggcc gcgtatgcgc gttggcaata
    10321 tgcgtgcaat tccaggtgag aagatactca acgccgttta cggcagccag cgcaacgtgc
    10381 agggcatcta cttctgcttt tttcggcagg gcgtattcct ggatcaaggc ttgcgccaac
    10441 tcctgcgcct gctgagaaac ttgcagcagt ggcaggctgt tgacaacagc aagacgccgc
    10501 tgcgccgctt ctggatgacc gcatccggct tctgaaacaa ccagctcaga agccaccagg
    10561 ctgtaatggt ctttttgtgt ttcccaccat tccgccgtga cactttggtt ggccatggca
    10621 cgcaggtcgt tgcttggacg tgccgtcaag tagctcagaa cggatgtttc gatgtaaaca
    10681 gatgacttca ttgtgctctc agcgctgatg gtgcgcagcg gagctgcaca ctctacggga
    10741 ctgggacgaa aaagccaccc ttgaggtggc tttcttgttt ctgcctcggc ctacgtaggc
    10801 ttccctttga atcgaatttc atcggacagc ccgttctctt gggcccaact tccgatcttc
    10861 tggctcagca cttcgatgta gtcggagcct tctgggaagg tttctcgatc aaagggctca
    10921 tgttggtcac cgctcaacag ttcccccaag agcttctgga caatttcact caaggcagcc
    10981 tcatttgtga tgcgacggta tgctgtaaaa ggcttgtctt tcgactcttc ttcaccgcca
    11041 aggaatcgct cgaaatccgg gacactaact cgatgccgca ccgtcacccc tgatgtgcga
    11101 gccttcacaa tagcgtcccg aatcttcttg ttctcagtcc acattccgtt attgtcccca
    11161 ttcttcttga atggtgggtc gcagtcatga acgatcccga agtcaatctt gaagtgctcc
    11221 attactttta ccagtggagc aaggattgct ttgcctcgcg cacggatcac ggttacgctg
    11281 ttcaatagtt cgtgctgctt tttgattact gcagccatga atgcggcatg ttcggtatcg
    11341 ccctcaacca gtaccgggta ggagccgaaa aatatttcag cgaagctcgg atcaatgtgc
    11401 tggagcgcct gcaatcgttg cttctcatca ccttc
//
LOCUS       DAOQIE010000097        11384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2832_length_11384_cov_7.079354, whole genome shotgun sequence.
ACCESSION   DAOQIE010000097 DAOQIE010000000
VERSION     DAOQIE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2832_length_11384_cov_7.079354"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK144_07755"
     CDS             complement(<1..311)
                     /locus_tag="PK144_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNF65660.1"
                     /translation="MASHAGFVEYVCAQAGLGPALGHRKMFGEYALYLDGRVVALICD
                     DQLFLKPSAAGEALLGRAPAHHPYPNARAHFRLDAELDTPGRLQQLLIATAAALPLPP
                     P"
     gene            complement(336..905)
                     /locus_tag="PK144_07760"
     CDS             complement(336..905)
                     /locus_tag="PK144_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HNF65661.1"
                     /translation="MNPILPRLLGLLLPLLAVTLPAFAADAPTPAETRAAIRQMRSET
                     LKTLYALEPKARTQVRGAAGYAVFSSTGVHVLFIGGGGGRGVVHDNLTGKDVWMRSAS
                     VGVGLGLGARELNLVLIFRKRDTLKRFMEQGWQFGGEASATARAGSSGSDVTDIEARD
                     GILIYPLSQDGLMARGAIEGTKYWVDPDL"
     gene            complement(902..2194)
                     /locus_tag="PK144_07765"
     CDS             complement(902..2194)
                     /locus_tag="PK144_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF65662.1"
                     /translation="MSRRLRLWLLIAAALIASVYLLPRWTPELLLGYFRNGEPIDRFT
                     LLLAAVLLMPALFERLHLPGAVGLLVAGVLFGPSVLAIGNPENSTVELFLSIGRVFLM
                     FIAGLEVDLRLFHATRLRSFGYGLATFALPMLAGIGVGWLFDFGLNACFLIGSLLASH
                     TLLGLPIASRLGLARSEVVTVTLGATVFTDVASLLVLAVCISVHTTGFTWLSLLEQLF
                     SLGVYGVLVLIGLPWLGVRFVRRWRHREAPMFLFAVLCLLFAAMGAKAIELEDIVGAF
                     FAGLAVNRVIGHGPVRGRIEFLGATLFIPFFFVIIGYRLDLPVFAATLQTSLPFVATI
                     IAALLLGKLAAALVTGPVYGYARRETLLMWSLSLPQVAATLAVALTAYNTLNADGERL
                     ITETVLNSVIVLMVVTSVLGPLLTERFATTLRHRDARP"
     gene            2475..3032
                     /locus_tag="PK144_07770"
     CDS             2475..3032
                     /locus_tag="PK144_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNF65663.1"
                     /translation="MNIKDELVRHYHWLRVYGLNDSHSGNASVREDDTVWVTPTGCCA
                     DLLSSGDLVACDFEGRIGRGASLDARLHVAVYGSNPRARAILHSHGPYTVAMTMDNEE
                     FVPADFEGQYYFGRVPVITIPFDRYKELAPQKVAEVLAEVPIAVVRGHGVFAAAENIN
                     LAYKWTCSLELSAKTAFIAQQAGKI"
     gene            complement(3107..4450)
                     /locus_tag="PK144_07775"
     CDS             complement(3107..4450)
                     /locus_tag="PK144_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNF65664.1"
                     /translation="MLLPERWIAACVPRPTDTATHASVRRLGRILIAVFEAVFDGHLL
                     DRAASLAYTTLLSLVPLLAVSFSVLQAFGAKDVLEPLLRDALTPLGGQAALVGDRILE
                     FVGKMQVGVLGSLGIAMLLYTVITTVHKIELALNAIWDVAEPRRPMRRIADYLSVTFA
                     GPVLAFGAIGLTDTVRGAFAGLAGRGYLTLADLGGALLALVPHALGMAAFALLYGLLP
                     NTRVRPGAAIAGSLFAVTVWSLAGRLFASLLSDSNNYSAIYSGFAGAVLFILWLNVNW
                     MIVLLGATVSCAWQAPGALNAGKPDGHAAREALALALMRELGRIHREGLPPPIADELA
                     ERLCARQRAIRDLLARLREDGLIIESDTCGWLPARDNAHLEVRAILASVRGPLPAEAA
                     GATLLAPGERALTDCYTGCTLDRLIDLPDDAAPPAPGIESRPAARPNVRPRLGKA"
     gene            complement(4479..5120)
                     /gene="pdxH"
                     /locus_tag="PK144_07780"
     CDS             complement(4479..5120)
                     /gene="pdxH"
                     /locus_tag="PK144_07780"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNF65665.1"
                     /translation="MTTPLADLRRNYTRGTLDVTEVAADPLAQFGRWFEEAVAAQVPE
                     PNAMTLATVAPDGRPQARVMLLKACDAEGFTFFTNYGSHKAAALAAHPQAALCFWWIE
                     LERQVRVEGRIEPLPRADSAAYFAVRPRDSQIGAWASRQSQPVADRAALEAQFAEQAA
                     RWDGREVELPQHWGGYRLHPGRVEFWQGRPGRMHDRIVYVRTGDAWQIGRLQP"
     gene            5309..5536
                     /locus_tag="PK144_07785"
     CDS             5309..5536
                     /locus_tag="PK144_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HNF65666.1"
                     /translation="MDTQTHNLSTLFAQLGLPEDPAGIDSFIREHPLPPDMLVADAPF
                     WNQTQAKFLHDALREDSDWAEMVDELNVRLH"
     gene            complement(5616..6794)
                     /locus_tag="PK144_07790"
     CDS             complement(5616..6794)
                     /locus_tag="PK144_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF65667.1"
                     /translation="MSDAERPARILPVIIASQFAGSSLWFAGNAILPELQRDWGLAAD
                     APGHVTAAVQFGFIAGTLVFALLALADRFSPRAVFLVCALAGAGANLALVALPPSFAA
                     LLLLRFATGFCLAGIYPVGMKIAAGWYASGLGRALGWLVGALVVGTAFPHLLRGLGAA
                     LPWAGVLAGVSACAALGGLAMYWLVPDGPQLKRGARFDPGAVRTIFAGRELRSAAFGY
                     FGHMWELYTWWAFLPAIVAAWGVQHGTTPAVSLWSFAAIAAGALGCIIGGELSPRWGS
                     ARVAQVQLAVSCACCLLAPLAFGLPGPLFFAYLLVWGITVVGDSPQFSTLTARAAPPA
                     YLGSALTWVTAIGFGITVVSIQLFGSLVQHWPIAWLLPLLALGPLPGLLAIRPLAGQR
                     "
     gene            7051..7629
                     /locus_tag="PK144_07795"
     CDS             7051..7629
                     /locus_tag="PK144_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF65668.1"
                     /translation="MNTAASDDLQTLIARVALGDGAAFRHLYELCAPRMLGVALRILV
                     RRDRAEDAVQEAFVKVWQRASDYSATAGSPLAWLTAIVRHQALDLLRSDKRRHTESTD
                     DDDEDGPGRLDPPDERPDPLGLLEQAIDGLRLRVCFDVVPEQQRRCLALAYYHGLTHS
                     ELAEHIAAPIGSVKVWLRRGLDRLRRCLEAAP"
     gene            7626..8411
                     /locus_tag="PK144_07800"
     CDS             7626..8411
                     /locus_tag="PK144_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNF65669.1"
                     /translation="MNYDRPPLIDHLAAAYVLGTLSARARRRFERLCRTLPSAADAVR
                     DWERRLGGLAEPVPPVAPSPQLWHAIERRTLGVREHPETARQPEARTRRERSESSRLG
                     DWLRPLLGFACGLLLAVGLVRLYPQGFVPGEPVDETALPASYIGLLTNAEGQPVLLAS
                     IRRHGRELQFKVLKPLTVPAGKVAVLWALAPNAAPAVVGVVPFAGGETAAPLAKATLT
                     LAGSAEQLLSNVPKLALSFESAPPAAGAAPSPFALEGNCVKLW"
     gene            8452..10488
                     /locus_tag="PK144_07805"
     CDS             8452..10488
                     /locus_tag="PK144_07805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNF65670.1"
                     /translation="MTPQAVVTEAVRDLPGGAQRITAPAAIRTAYRPDIDGLRAVAVL
                     LVVIHHAFPQSGGGFIGVDVFFVISGYLITGILAGDIRQSRFRLAGFYARRIRRIYPA
                     LLVVLLASLLAGWFLMLPNELATLGRHVAGGSGFVANVLYWLQSGYFDRQAELQPLLH
                     LWSLGVEEQFYIVWPLLLAGLMRLPPRRCLQAVGALLLLSLLGCIVVSEHDLSAAFFL
                     PHTRFWELAAGGWLALQQAGQRPAADGVPATGTGRWPQHAASVAGLSAIGLGAVLIDR
                     HSAFPGAWALLPVGGALLLIQAGPAACINRHLLARAPMRWIGRISFSLYLWHWPLLVY
                     GRIWLVGEPDWPQRLALVAVSVLLAWLSYRWIETPFRSAPTRRAPRPWSPGPVRFLAG
                     MMAATFCAGVVLHLARGYPDRMPDKRDYIAFFDDYRYTRTHDLLRFDHHECNFYDIES
                     GRAKTAIAATCLRPAAAHRRSVLLWGDSHAQHLAYGLDRTLPADVALLHVGASGCPPN
                     VDDVHPDPLDSCNRANRLAWQAIGELHPDVVMLAQQGGHSITQYDALIARLHAGGVAQ
                     VVLIGPVPHWQPFLYQVVLKKYWQATPAQIGSNLDPAMFGLDAGLQRRYAGMPGVTYV
                     SMIGPLCHDGACLAYLDGDRKEKLMTYDYAHFTLPASEFVVRTVVYPAMSGLLN"
     gene            10717..11295
                     /locus_tag="PK144_07810"
     CDS             10717..11295
                     /locus_tag="PK144_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011270027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNF65671.1"
                     /translation="MSSIAIVFFSGYGHTLKQAEAVKAGAAGVEGAEVAFLQIDSDGN
                     LPESAWETLAAADAILFGSPTYMGGPAWQFKKFADASSRPWFTKAWKDKLAAGFTNSA
                     STNGDKYSTIQYFWTLSQQHGQIWVGTGLLPANGKANGPADVNWTAGFGGAMSISPFD
                     ASPDEAPRSGDLETARLLGERVAQLAGRFAKG"
BASE COUNT         1726 a         4031 c         4091 g         1536 t
ORIGIN      
        1 ttcgggggcg gcagcggcag ggccgcggcg gtcgcgatca gcagttgctg caggcgcccg
       61 ggcgtgtcga gttcggcgtc gagccggaaa tgcgccctgg cattcgggta gggatggtgt
      121 gccggcgcgc gcccgagcag cgcctcgccg gcggcgctcg gcttcaggaa cagctggtcg
      181 tcgcagatca gcgcgacgac ccggccgtca agatagagcg cgtattcgcc gaacatcttg
      241 cgatggccca gcgccggccc gagcccggcc tgcgcgcaga cgtattcgac gaaaccggcg
      301 tgcgaggcca tgggggcgtc ctccgggctg cgggttcaga gatccggatc gacccagtac
      361 ttcgtaccct cgatcgcgcc tcgggccatc agcccgtcct ggctgagcgg gtagatcagg
      421 atgccgtcgc gggcttcgat atcggtcaca tcgctgccgc tgctgccggc gcgggccgtg
      481 gcgctcgcct cgccgccgaa ctgccagccc tgctccatga agcgtttcag cgtgtcgcgc
      541 ttgcggaaga tcagcaccag gttcagctcc cgggcaccga gcccgaggcc gacgccgacg
      601 ctggccgagc gcatccagac gtccttgccg gtcaggttgt cgtgtacgac gccgcgcccg
      661 ccgccgccgc cgatgaacag cacgtgcacg ccggtgctgc tgaacaccgc gtagccggcc
      721 gcgccgcgca cctgcgtgcg tgccttcggc tccagcgcgt acagggtttt cagggtttcg
      781 ctgcgcatct ggcggatcgc ggcccgggtt tcggccggcg tcggtgcatc ggcggcgaag
      841 gccggcagcg tcacggcgag cagcggcagc agcaggccca gcagtcgggg aaggatcgga
      901 ttcatgggcg ggcatcccgg tggcgcagcg tggtggcgaa gcgctcggtc agcagcggac
      961 cgagcaccga agtcacgacc atcagcacga tgacgctgtt cagcaccgtt tcggtgatca
     1021 gccgttcgcc gtcggcattc agcgtgttgt aggccgtcag cgccacggcc agcgtcgcgg
     1081 ccacctgcgg cagcgacagc gaccacatca gcagcgtctc gcggcgcgca tagccgtaca
     1141 ccggcccggt caccagcgcg gcggcgagct tgccgagcag cagcgcggcg atgatcgtgg
     1201 cgacgaacgg caggctggtc tgcagcgtcg ccgcgaacac cggcaggtcg agccggtagc
     1261 cgatgatcac gaagaagaac gggatgaaca gcgtcgcgcc gaggaattcg atcctgccgc
     1321 gcacggggcc gtggccgatc acgcggttga ccgccagccc ggcgaagaag gcaccgacga
     1381 tgtcctccag ctcgatggcc ttggcgccca tcgccgcgaa cagcaggcag agcaccgcaa
     1441 acaggaacat cggcgcctcg cgatggcgcc agcggcgcac gaagcgcacg ccgagccatg
     1501 gcaggccgat cagcaccagc acgccgtaga cgccgagcga gaacagctgc tccagcaggc
     1561 tcagccacgt gaagccggtc gtgtgcaccg agatgcacac ggccagcacc agcagcgagg
     1621 cgacatcggt gaacaccgtg gcgccgagcg tgacggtgac cacctcgctg cgcgccaggc
     1681 cgaggcggct cgcgatcggc aggccgagca gcgtgtgcga ggcgagcagc gagccgatca
     1741 ggaagcaggc gttcagcccg aagtcgaaca gccagccgac gccgatgccg gcgagcatcg
     1801 gcagcgcgaa ggtcgcgagt ccgtagccga aggagcgcag ccgcgtcgcg tggaacagcc
     1861 gcaggtcgac ctccagtccg gcgatgaaca tcaggaacac gcggccgatg ctcaggaaca
     1921 actcgacggt gctgttttcg ggattgccga tcgccagcac cgatggcccg aacagcacgc
     1981 cggccaccag caggccgaca gccccgggca ggtgcaggcg ctcgaacagc gccggcatca
     2041 gcagcaccgc ggccagcagc agcgtgaagc ggtcgatcgg ctcgccgttg cggaaatatc
     2101 cgagcagcag ctcgggggtc cagcgtggca gcagatacac gctggcaatg agtgcggcgg
     2161 cgatcagcag ccacagacgc agacgacgcg acatgcgcag acactcccgg agggatttcg
     2221 gggttgcggc caccaccggg tggtcgcgcg tcgcagtatg gacgaatgtc gctgcgctgc
     2281 cgccgcgccg ttgtgccggt cagaccgcca ccggggccgg ccttccacac ccggggcttt
     2341 tcccgcacca tagcgccccc cgcggcccct gccgccgccc cctgtttccg ccgctgcggc
     2401 tgttgccgtc gccgcgcggc ccgccgcgac gctgcctgcg cgcccgaatc cgctgaactc
     2461 cggacttcct cccgatgaac atcaaagacg aactggttcg ccactatcac tggctgcgcg
     2521 tctacggcct gaacgactcg cacagcggca acgcctcggt gcgcgaggac gacacggtct
     2581 gggtgacgcc gaccggctgc tgcgccgatc tgctgagcag cggcgatctc gtcgcctgcg
     2641 acttcgaggg ccgcatcggc cgcggcgcct cgctcgatgc gcggctgcac gtcgccgtct
     2701 acggcagcaa tccgcgcgcg cgcgccatcc tgcacagcca cgggccgtac acggtggcga
     2761 tgacgatgga caacgaggag ttcgtgccgg ccgacttcga gggccagtac tacttcgggc
     2821 gcgtgccggt gatcacgatc ccgttcgacc gctacaagga gctggcgccg cagaaggtcg
     2881 ccgaggtgct ggccgaggtg ccgatcgcgg tcgtgcgcgg ccacggcgtg ttcgcggccg
     2941 ccgaaaacat caaccttgcc tacaagtgga cctgctcgct ggagctgtcg gcgaagaccg
     3001 ccttcatcgc gcagcaggcc ggcaagatct gatgccggtc cgatcctgac cggatcccgc
     3061 cggcacagcc ggtccgcggc tgcccgggtg tcgcgatcgg cagggctcag gccttgccga
     3121 gccgcgggcg cacgttcggg cgggcggccg ggcgggattc gatgccgggt gccggcggtg
     3181 ctgcgtcatc cggcaggtcg atcagccggt cgagcgtgca gccggtgtag cagtcggtca
     3241 gcgcgcgttc gccgggcgcg agcagcgtcg cgccggcggc ctcggccggc agcgggccgc
     3301 gcacgctggc gaggatggcg cgcacttcga gatgggcgtt gtcgcgcgcc ggcagccagc
     3361 cgcaggtgtc gctctcgatg atcaggccgt cctcgcgcag ccgcgccagc aggtcgcgga
     3421 tcgcgcgctg gcgggcgcag agcctctcgg cgagctcatc ggcgatcggc ggcggcaggc
     3481 cctcgcggtg gatgcggccg agttcgcgca tcagcgccag cgccagcgcc tcgcgcgcgg
     3541 cgtggccgtc gggcttgccg gcgttcagcg cccccggtgc ctgccaggcg caggacaccg
     3601 tcgcgccgag cagcacgatc atccagttga cgttcagcca caggatgaac agcacggcgc
     3661 cggcgaagcc cgagtagatc gccgagtagt tgttggaatc gctgagcagg ctcgcgaaca
     3721 gccgcccggc cagcgaccag accgtcaccg cgaacaggct gccggcgatc gccgcgcccg
     3781 ggcgcacgcg cgtgttcggc agcaggccgt agagcagcgc gaaggccgcc atgccgaggg
     3841 cgtgcggcac cagcgcgagc agcgcgccgc cgagatcggc gagcgtcagg tagccgcgcc
     3901 cggcgagtcc ggcgaaggcg ccgcgcacgg tgtcggtcag gccgatggcg ccgaaggcga
     3961 gcaccgggcc ggcgaaggtc accgacaggt aatcggcgat gcggcgcatc ggccggcgcg
     4021 gttcggcgac atcccagatc gcgttcagcg ccagctcgat cttgtgcacg gtcgtgatca
     4081 ccgtgtagag cagcatcgcg atgccgagcg aaccgagcac gccgacctgc atcttgccga
     4141 cgaactccag gatgcggtca ccgaccagcg ccgcctgacc gccgagcggc gtcagcgcat
     4201 cgcgcagcag cggttcgagc acgtccttgg cgccgaaggc ctgcagcacc gaaaagctca
     4261 ccgccagcag tggcaccagc gacagcagcg tcgtgtaggc gaggctggcg gcgcgatcga
     4321 gcaggtggcc gtcgaacacg gcctcgaaga ccgcgatcag gatgcgcccg agccggcgaa
     4381 ccgaggcgtg cgtggcggta tcggtcggcc gcggcacaca ggcagcgatc cagcgttcgg
     4441 gcagcagcat ccgtcctcct cgatccgcgc tctcgacttc agggctgcag gcgtccgatc
     4501 tgccaggcgt cgccggtgcg cacgtagacg atgcgatcgt gcatgcgtcc cggccggccc
     4561 tgccagaact cgacgcgccc ggggtgcagg cggtagccac cccagtgctg cggcagttca
     4621 acctcccgac cgtcccagcg cgccgcctgt tccgcaaact gcgcttcgag cgcggcgcga
     4681 tcggcgaccg gctggctctg gcgcgaggcc caggcgccga tctggctgtc gcgcgggcgc
     4741 acggcgaagt aggcggccga gtcggcgcgc ggcaagggtt cgatgcggcc ctcgacgcgc
     4801 acctgccgct ccagctcgat ccaccagaag cagagcgccg cctgcggatg cgcggcgagt
     4861 gcggcggcct tgtggctgcc gtagttggtg aagaacgtga agccttcggc gtcgcaggcc
     4921 ttcagcagca tcacgcgcgc ctgcggccgg ccgtcggggg cgacggtggc gagtgtcatc
     4981 gcgttcggtt cgggcacctg cgcggcgacc gcctcctcga accagcgccc gaactgggcg
     5041 agcggatcgg cggcgacttc ggtgacatcg agcgtgccgc gggtgtagtt gcggcgcaga
     5101 tcggcgagcg gggtggtcac ggcggacact ccttatggat ggagaaggga aacggaaagg
     5161 caagggccgc gcaggcatcg gccggatgcg cggccggagg aacggttgcg gcgggcgggg
     5221 tctataaagg tccgggtacg ggaggctgct cccgtaatcc cagagtccgg atgaccgccc
     5281 agatgactgc cgcgaggagt tcgacatgat ggacacccag acccataacc tgagcaccct
     5341 gttcgcccag ctcggcctgc cggaagaccc ggccggcatc gacagcttca tccgcgaaca
     5401 tccgctgccg ccggacatgc tggtggccga tgcgccgttc tggaaccaga cccaggcgaa
     5461 gttcctgcac gatgcgctgc gcgaggattc ggactgggcc gagatggtcg atgaactgaa
     5521 cgtccgcctg cattgagcat ggtcagccgc cgctgcggcg gcgcatgtcc cgacggcccg
     5581 cagccagcgg ctgcgggccg tcgcatgtgc gggcctcagc gctgtccggc cagcggccgg
     5641 atcgccagca gacccggcag cgggccgagc gccagcagcg gcagcagcca ggcgatcggc
     5701 cagtgctgca cgaggctgcc gaacagctgg atgctgacga cggtgatgcc gaagccgatc
     5761 gccgtcaccc aggtcagcgc cgagccgaga tacgccggcg gtgcggcgcg cgcggtcagc
     5821 gtcgagaact gcggcgagtc accgacgacg gtgatgcccc agaccagcag ataggcgaag
     5881 aacagcggcc ccggcagtcc gaaggcgagc ggcgcgagca ggcagcacgc gcacgacacc
     5941 gcgagctgca cctgcgcgac gcgcgcgctg ccccagcgcg gtgacagttc gccgccgatg
     6001 atgcagccga gcgcgccggc ggcgatcgcc gcgaagctcc agagcgacac cgcgggcgtg
     6061 gtgccgtgct gcacgcccca ggcggcgacg atggccggca agaaggccca ccaggtgtac
     6121 agctcccaca tgtgaccgaa gtagccgaag gccgccgagc gcagctcgcg gccggcgaag
     6181 atcgtgcgca ccgcgccggg atcgaagcgc gccccgcgct tcagctgcgg gccgtccggc
     6241 accagccagt acatcgccag tccgccgagc gccgcgcagg ccgacacgcc ggcgagcacg
     6301 ccggcccagg gcagcgccgc gccgagtccc cgcagcaggt gcgggaaggc cgtgccgacc
     6361 accagcgcac cgaccagcca gccgagcgcg cgcccgagtc cgctcgcata ccagccggcc
     6421 gcgatcttca tgccgaccgg gtagatgccg gccagacaga agccggtcgc gaagcgcagc
     6481 agcagcagcg cggcgaagct cggcggcagc gccaccagcg cgagattggc accggcaccg
     6541 gcgagcgcgc agacgaggaa gaccgcacgc ggcgaaaagc ggtcggcgag cgcgagcagc
     6601 gcgaacacca gcgtgccggc gatgaagccg aactgcacgg cggcggtcac gtgcccgggc
     6661 gcatcggcgg cgagccccca gtcgcgctgc agctcgggca ggatggcgtt gccggcaaac
     6721 cacagcgagg agccggcgaa ctgcgaggcg atgatcaccg gcaggatgcg ggcagggcgt
     6781 tcggcgtcgg acatgcgggc gacctgcgca ggcgggggag ggagaaggca gaagagcggc
     6841 gatcgtagcc gctggcggcg gtcgctgcgc cggctgcatc ggagccagac ggcggcgtgg
     6901 ccccgctgcg cagcggccgg aagccggcca catgagcgga ccgtcactgg caggccggcc
     6961 ggcgctgttt atgctgcacg gcagcggacc ggtgtccgtg cagggggaag gtgcgatgtg
     7021 catgacgacc agccgcagcg gagcggcgcg atgaacacgg cagccagcga cgatctgcag
     7081 accctgatcg cccgtgtcgc gctcggtgac ggcgcggcgt tccgccacct gtacgaactc
     7141 tgcgcgccgc gcatgctcgg cgtggccctg cgcatcctcg tgcgccgcga ccgcgccgag
     7201 gacgccgtgc aggaagcctt cgtgaaggtc tggcagcgcg cgagcgacta ttcggcgacc
     7261 gccggctcgc cgctggcgtg gctgacggcg atcgtgcgtc atcaggcgct cgacctgctg
     7321 cgcagcgaca agcgccggca caccgaatcg accgacgacg atgacgagga cggccccggc
     7381 cgcctcgatc cgcccgacga gcggcccgat ccgctcggcc tgctcgaaca ggcgatcgac
     7441 ggcctgcgcc tgcgcgtgtg cttcgatgtc gtgcccgagc agcagcgtcg ctgtctggcg
     7501 ctcgcgtact atcacgggct cacgcattcg gagctggccg agcacatcgc cgccccgatc
     7561 ggcagcgtca aggtctggct gcgccgcggc ctcgaccggc tgcgccgctg cctggaagcc
     7621 gccccatgaa ctacgaccgt ccgccgctga tcgatcacct cgccgctgcc tatgtgctcg
     7681 gcacgctgtc ggcgcgcgcg cgccggcgct tcgagcggct ctgccgcacg ctgccttcgg
     7741 ccgccgacgc cgtgcgcgac tgggagcgcc ggctcggcgg cctcgccgaa ccggtgccgc
     7801 cggtcgcacc gtcgccgcag ctctggcacg cgatcgagcg acgcacgctc ggcgtgcgcg
     7861 aacacccgga aaccgcccgt cagcccgaag cgcgcacgcg ccgggaacgc agcgaaagca
     7921 gccggctcgg cgactggctg cggccgctgc tcggtttcgc ctgcggcctg ctgctcgccg
     7981 tcgggctggt gcggctgtac ccgcagggct tcgtgccggg cgagccggtc gacgagaccg
     8041 cgctgccggc gagctacatc gggctgctga ccaacgccga aggccagccg gtgctgctcg
     8101 cgagcatccg ccgccacggc cgcgagctgc agttcaaggt gctgaaaccg ctgacggtgc
     8161 cggccggcaa ggtggcggtg ctgtgggcgc tggcgccgaa cgccgccccg gccgtggtcg
     8221 gcgtcgtgcc gttcgcgggc ggcgagaccg ccgcgccgct cgcgaaggcg acgctgacgc
     8281 tcgccggcag cgccgagcag ttgctgagca acgtgccgaa gcttgcgctg agcttcgaat
     8341 ccgccccgcc ggccgccggc gccgccccgt cgccgttcgc gctcgaaggc aactgcgtga
     8401 agctctggta ggtcgtccgc agccgcacgc gctgcagggc agggaaccgg catgacgccg
     8461 caggcggtgg tgacggaggc ggtccgggac ttgcccggcg gggcgcagcg gatcactgcg
     8521 ccggcggcga tccgcacggc ctaccgcccc gacatcgacg gcctgcgcgc cgtcgccgtg
     8581 ctgctggtgg tgatccatca cgcctttccg cagagcggcg gcggcttcat cggcgtcgac
     8641 gtgttcttcg tgatctccgg ctacctgatc accggcatcc tcgccggcga tatccgccag
     8701 tcgcgctttc gcctcgccgg cttctacgcc cggcgcatcc gccgcatcta tccggcgctg
     8761 ctcgtggtgc tgctggcgag cctgctcgcc ggctggttcc tgatgctgcc gaacgaactc
     8821 gccaccctcg gccggcacgt cgccggcggc agcggcttcg tcgccaatgt cctgtactgg
     8881 ctgcagtccg gttacttcga ccggcaggcc gaactgcagc cgctgctgca cctgtggtcg
     8941 ctcggcgtcg aggagcagtt ctacatcgtc tggccgctgc tgctggcggg gctgatgcgc
     9001 ctgccgccgc gccgctgcct gcaggccgtc ggcgcgctgc tgctgctgtc gctgctcggc
     9061 tgcatcgtcg tcagcgagca tgacctgagc gcggccttct tcctgccgca cacccgcttc
     9121 tgggaactcg cggccggcgg ctggctggcg ctgcagcagg ccggacagcg gccggccgca
     9181 gacggcgtgc cggcgacagg taccggtcgc tggccgcagc atgccgcgag cgtggccgga
     9241 ctgtccgcga tcggactcgg cgccgtgctg atcgaccggc attcggcgtt tccgggcgcc
     9301 tgggcgctgc tgccggtggg cggggcgctg ctgctgatcc aggccgggcc tgcggcctgc
     9361 atcaaccgtc acctgctggc ccgggcgccg atgcgatgga tcggccggat cagcttctcg
     9421 ctgtatctgt ggcactggcc gctgctggtg tacggccgca tctggctggt cggcgaaccg
     9481 gactggccgc agcggctggc gctggtggcc gtgtcggtgc tgctcgcctg gctgtcgtac
     9541 cgctggatcg agacaccgtt tcgcagcgcc ccgacgcggc gggcgccgcg gccctggtcg
     9601 cccggaccgg tgcgcttcct ggccggcatg atggcagcga ccttctgcgc cggagtggtg
     9661 ctccatctcg cccgcggcta tccggatcgc atgccggaca agcgcgacta catcgccttc
     9721 ttcgacgatt accgctacac ccgcacgcac gacctgctgc gcttcgatca ccacgagtgc
     9781 aacttctacg acatcgagag cggccgcgcg aagaccgcga tcgccgcgac ctgcctgcga
     9841 ccggccgccg cgcaccggcg cagcgtgctg ctgtggggcg attcacacgc ccagcatctc
     9901 gcctacggcc tcgaccggac gctgccggcc gatgtcgcgc tgctgcacgt cggcgcctcg
     9961 ggctgtccgc cgaacgtcga tgacgtgcat cccgatccgc tcgacagctg caaccgtgcc
    10021 aaccgcctgg cctggcaggc gatcggcgag ctgcaccccg atgtcgtgat gctcgcccag
    10081 cagggcgggc attcgatcac gcagtacgac gcgctgatcg cacgcctgca cgcgggcggc
    10141 gtcgcgcagg tcgtgctgat cgggccggtg ccgcactggc agcccttcct gtatcaggtg
    10201 gtgctgaaga agtactggca ggccacgccg gcgcagatcg gcagcaacct cgatcccgcc
    10261 atgttcgggc tcgatgccgg cctgcagcgc cgctatgccg gcatgcccgg cgtgacctac
    10321 gtgtcgatga tcggcccgct gtgccacgac ggcgcctgcc tcgcctacct cgacggcgat
    10381 cgcaaggaaa agctgatgac ctacgactac gcccacttca cgctgccggc ctccgaattc
    10441 gtcgtgcgca ccgtcgtgta tccggcgatg tcggggctgc tgaactgaag gcgggccgca
    10501 cggcgccggt ggcagcaaat ccgggcggcg gatcggcagg cgggtgctgt tctgctgcca
    10561 tgcagcgctt gtctgcagtc ggatggccgg gccggtggtc acccgactgg cggtgccgca
    10621 gccgcagccg gtccgggtac ggccccgggc gtggagcgcg tggcgtccgc atttcctctc
    10681 tcgccaggtg cggccgaagg ccgacaggag tcctgcatgt ccagcatcgc gatcgtgttc
    10741 ttcagcggct acggccacac cctgaagcag gccgaggccg tgaaggccgg ggctgccggg
    10801 gtcgagggcg ccgaggtggc gttcctgcag atcgacagcg acggcaatct gccggaatcg
    10861 gcctgggaga cgctggccgc ggccgacgcc atcctcttcg gcagcccgac ctacatgggc
    10921 ggtccggcct ggcagttcaa gaagttcgcc gacgccagct ccaggccctg gttcacgaag
    10981 gcctggaagg acaagctcgc cgccggcttc acgaactcgg cgtccacgaa cggcgacaag
    11041 tactcgacca tccagtactt ctggacgctg tcgcagcagc acggccagat ctgggtcggc
    11101 accggcctgc tgccggccaa cggcaaggcc aacggtccgg ccgacgtgaa ctggaccgcc
    11161 ggcttcggcg gcgcgatgtc gatctcgccc ttcgacgcca gccccgacga agccccgcgc
    11221 agcggtgacc tcgaaaccgc gcgcctgctc ggcgagcgcg tcgcgcagct ggcggggcgc
    11281 ttcgcgaagg gctgaaagcc ggcggcatcg gccgcctgcg ttgcgcacgg cgttgctgtc
    11341 cggccccgct gcctttgccg gagaggggcc gggcgcagcg ggca
//
LOCUS       DAOQIE010000098        11382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2833_length_11382_cov_8.121038, whole genome shotgun sequence.
ACCESSION   DAOQIE010000098 DAOQIE010000000
VERSION     DAOQIE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_11382_cov_8.121038"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="phaC"
                     /locus_tag="PK144_07815"
     CDS             complement(<1..1016)
                     /gene="phaC"
                     /locus_tag="PK144_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HNF65672.1"
                     /translation="MTPSDSADSAGADPYAELLDYGRRLGAGLDHLHAGGAPELAATA
                     REVICRADKLTLYRYLPRVAAPQKTPLLIVYALVNRPTMTDLQPDRSLVRGLLDAGLE
                     VYLIDWGYPDRADRLLTLDDYVNGYLHDCVETVCARHGLPAIHVLGICQGGVLSLAYA
                     ALHPGRVRNLIPMVTPVDFHVPEFLLTKLVRPLDLELMIGTLGNMPGPLLNFLFLSLS
                     PFRQGRQKYVHVADLAEDPGALANFVRMEHWIYDCPDLAGTAFREFVTATFHDNRLVR
                     GDFEIGGRRVDPARLLMPMLNIYATRDHIVPPQSSQALRRLYGGRDYSEIAFDGGHIG
                     IYVSR"
     gene            complement(1013..1927)
                     /locus_tag="PK144_07820"
     CDS             complement(1013..1927)
                     /locus_tag="PK144_07820"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HNF65673.1"
                     /translation="MPDTPEGPADRLSELLAAHCHALQEWLRSGAAGRAPTPDAALAA
                     WRAANPDTGDTFERTLAQGLRFVGLVEELLRLGAATGQTDADATDWRRLIDSLQTLPF
                     GASADDGWQAAWRSLAGQLPATWAALLPWLQQGGSSPGLAAELHRGLPLPGLAPAASR
                     ALWLELAGAWQEYSAADAAYRTLLDEAAAAAAKRLRAEVGREPPASVRALYDRWVEAS
                     EHVWAKLIREPRYAETQARLVHAMLHCRRALQQLVAELAAHVDLPTRAEIDTLELRLH
                     EQRTRQRQLERELAALRSAFAGRGDAQP"
     gene            complement(1952..3403)
                     /locus_tag="PK144_07825"
     CDS             complement(1952..3403)
                     /locus_tag="PK144_07825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF65674.1"
                     /translation="MRTLILGVLLLSLGTQSLAQSIALPDMGDPSQQVLGPQDERRIG
                     QRILMKLQDAGLVLEDPLASEYLASLGSRLALHADGAGSPLRFFWVRDPELNAFALPG
                     GFIGVHTGLMLETRSESELAGVVAHEIAHVVQHHITRAYADRQTIGLPTMAAMLAGIA
                     LAAAGGGGGQIGQAVAVGSMAASAQRIVNFTRANEQEADRVGTDLLIRAGFEPNGMAS
                     FFGRLENLSGSAAQIPEFLRTHPLALNRAADTENRSLRTRPTGRTPGDELYYRYMRAR
                     IAALSVATPAQAVRDHERRAAEGGTDEADRYGYVLALERAGRLDDAARELAKLPRPAA
                     AALALRLEEARLALLTGNTARAWSTYEALRRAYGNDYVLTLSYGEALVTRGDPRLAIR
                     LLDEQLRRRPESAELQELYARAAEQTGDIAGKHLAMSQFYELSGDVQAAFDQAEIGLR
                     APNASGQQLAQLRQRAQRLRELNEYEKASRLPR"
     gene            3552..3791
                     /locus_tag="PK144_07830"
     CDS             3552..3791
                     /locus_tag="PK144_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNF65675.1"
                     /translation="MNTVIDAELDAAGLNCPLPILKTKKALAALAGGQVLRVVATDPG
                     SVRDFEAYTRQTGHALLESREQDGRFVFVIRKAGG"
     gene            3947..>4385
                     /locus_tag="PK144_07835"
     CDS             3947..>4385
                     /locus_tag="PK144_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65676.1"
                     /translation="MKSWIEARERFWEQYRAALPTDTDQAVDAWRSLRAQHLALWEKL
                     VHDTLAGQTAWVDRLFAQLSNAPAPERVVEFRGQFEQGIRQWLETQDRLWGELFTLMR
                     GEAPVAADVPVAPEAPAAEPAPVEPVSQAPAEPVYEQPAPVYEQ"
     assembly_gap    4386..4485
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4486..4815
                     /locus_tag="PK144_07840"
     CDS             <4486..4815
                     /locus_tag="PK144_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65677.1"
                     /translation="SQAPAEPVYEQPAPVYEQQAPVYEQQAPVYEQQAPVYEQQAPVY
                     EQQAPVYEQQAPVYEQQAPVYEQQTPVYEPMPSEPVADDTVADAGESQGEGGDSAPKT
                     RRRRTSK"
     gene            complement(4900..5679)
                     /locus_tag="PK144_07845"
     CDS             complement(4900..5679)
                     /locus_tag="PK144_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNF65678.1"
                     /translation="MQLIAVQTDIRWEDKPANHEHVRELLAAAAPPPGALAVLPEMCM
                     TGFSMKVAKIDEGEARPGEQFLSALAQEFGIFICAGLVTRADDGRGLNQSVTWAPDGS
                     ELARYSKCHPFSFAHEDRHYAKGDGPVLYEWAGLRVAPTICYDLRFPELYRIAAGRGA
                     QLYTVIANWPQRREAHWRALLVARAIENQAWVIGVNRCGDDPWLHYGGASLIIDPRGE
                     IIADAGSGPGTIRAEVHLDALLDYRASFPALADMRGEFVPV"
     gene            5776..7179
                     /gene="asnS"
                     /locus_tag="PK144_07850"
     CDS             5776..7179
                     /gene="asnS"
                     /locus_tag="PK144_07850"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNF65679.1"
                     /translation="MTDLPTIATLLGGTVAVGTPVTVQGWVRTRRDSKAGLSFVAVHD
                     GSCFDAIQCVVPDTLANYRSEVLHLGSGCSVRVTGTLVASGGKGQTVEVQAAALEVIG
                     LVEDPETYPVQPKPHTFEYLRTVAHLRPRTNTFGAVARVRHCLAQAIHRFFSAHGYCW
                     VHTPIITASDCEGAGELFRVSTLDFANLPRTAEGRIDYAQDFFGREAFLTVSGQLNVE
                     AYCLALSRVYTFGPTFRAENSNTSRHLAEFWMVEPEIAFADLAADADLAEALLKSVFR
                     AVLDERGDDLAFFDDRILKGCIARLEALVEQPFARIDYSEAVRVLEGCGETFQFPVTW
                     GADLQSEHERFLAERHVGGPVVVMNYPKAIKSFYMRLNDDGRTVAAMDVLAPGIGEII
                     GGSQREERLDVLDARMRECGLDPAGYGWYRDLRRYGTVPHAGFGLGFERLVSYVTGMA
                     NVRDVIPFPRTPGSAEF"
     gene            complement(7198..7353)
                     /locus_tag="PK144_07855"
     CDS             complement(7198..7353)
                     /locus_tag="PK144_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65680.1"
                     /translation="MSMLAIIGLLLPSATVAVFAEWRARRIGAGTARRKAAEPWQRSG
                     SLFFSHD"
     gene            complement(7562..7637)
                     /locus_tag="PK144_07860"
     tRNA            complement(7562..7637)
                     /locus_tag="PK144_07860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7602..7604),aa:Asn,seq:gtt)
     gene            complement(7726..7801)
                     /locus_tag="PK144_07865"
     tRNA            complement(7726..7801)
                     /locus_tag="PK144_07865"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7766..7768),aa:Asn,seq:gtt)
     gene            complement(7894..9093)
                     /locus_tag="PK144_07870"
     CDS             complement(7894..9093)
                     /locus_tag="PK144_07870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNF65681.1"
                     /translation="MSLFSAVEMAPRDPILGLNEAYNADPRATKVNLGVGVYFTDAGR
                     IPLLRAVQEAEKARIAAAAPRGYLPIEGIAAYDKLVQELLFGTASPLLAEGRVVTAQA
                     LGGTGALKIGADFLRRLLPGASVAISDPSWENHRALFESAGFEVGSYRYYDAPNHGVD
                     LAGMLADLQALPANSIVVLHACCHNPTGADLTPAQWQQVLAVLAERGHVPFLDIAYQG
                     FGDGIDADAVAVRLFAESGLPFFVASSFSKSFSLYGERVGALSIVTGSRDESARVLSQ
                     LKRVIRTNYSNPPTHGASIVAAVLGSHELRALWEQELGEMRERIRAMRSGLVAGLAAA
                     GVPGDYGFVQRQRGMFSYSGLSAAQVERLKTEHGIYAVGTGRICVAALNTTNLPTVAA
                     AIAEVLR"
     gene            9225..11255
                     /gene="uvrB"
                     /locus_tag="PK144_07875"
     CDS             9225..11255
                     /gene="uvrB"
                     /locus_tag="PK144_07875"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNF65682.1"
                     /translation="MQRFELVTDYQPAGDQPAAIDALVRGLEDGLAHQTLLGVTGSGK
                     TFTMASVIARVQRPAMILAQNKTLAAQLYGEMKAFFPHNAVEYFVSYYDYYQPEAYVP
                     ASDTFIEKDASINAHIEQMRLSATKAILERADTIIVASVSSIYGLGDPELYLQMILHL
                     SRGERIDQRDILKRLTALQYVRNDAVLERGSFRVRGEVIDVHPADSETEAVRIELFDD
                     EIEQLSYFDPLTGVVARRVPRLTIHPKTHYVTPRARILETVDAVKLELAARVTELERA
                     GRLLEAQRIEQRTRFDLEMMLELGYCSGIENYSRYLSGRQAGEPPPTLFDYLPPHALL
                     IIDESHVTVPQLGGMYRGDRSRKETLVEYGFRLPSALDNRPLRFDEFERLAPQTIFVS
                     ATPGNYEGEHAGQVVEQLVRPTGLVDPQVEVRPARTQVDDLMSEIVARVRLDERVLVT
                     TLTKRMAEDLTEYLAEHGVRVRYLHADIETVERVEIIRDLRLGEFDVLVGINLLREGL
                     DMPEVSLVAILDADKEGFLRSERSLIQTIGRAARHLNGRAILYADRITDSMRRALDET
                     GRRRVKQEAHNAAHGIVPRSVRKAVADILEGGIPGAGRSRREPAAEAGLAESRGPYRV
                     LTPEQAAKKLRELEARMLRHARDLEFEEAARLRDEIVRIRETTFGAGQAAAG"
BASE COUNT         1696 a         3826 c         4113 g         1647 t
ORIGIN      
        1 cggctgacgt agatgccgat gtggccgccg tcgaaggcga tctcggaata gtcgcggccg
       61 ccgtagagcc ggcgcagcgc ctgcgaggac tgcggcggca cgatgtgatc gcgcgtcgcg
      121 tagatgttca gcatcggcat cagcagccgg gccggatcga cgcgccggcc gccgatctcg
      181 aagtcgccgc gcaccaggcg gttgtcgtgg aaggtcgcgg tcacgaactc gcggaaggcc
      241 gtgccggcga ggtccgggca gtcgtagatc cagtgctcca tgcgcacgaa gttggcgagg
      301 gccccggggt cttcggcgag gtcggcgacg tgcacgtatt tctgccggcc ctgacggaag
      361 gggctcaggc tcaggaacag gaagttcagc agcggccccg gcatgttgcc gagggtgccg
      421 atcatcagct cgagatcgag cgggcgcacg agcttggtca gcaggaactc cggcacgtgg
      481 aaatcgaccg gcgtcaccat cgggatcagg ttgcgcacgc gcccggggtg cagggccgcg
      541 taggcgaggc tcagcacgcc gccctggcag atgccgagca cgtggatcgc cggcaggccg
      601 tggcgggcgc agaccgtctc gacgcagtcg tgcaggtagc cgttgacata gtcatcgagc
      661 gtcagcagcc ggtcggcgcg gtccggatag ccccagtcga tcaggtagac ctcgagcccg
      721 gcatcgagca ggccgcgcac cagcgagcgg tcgggctgca ggtcggtcat cgtcgggcgg
      781 ttgaccagcg cgtagacgat cagcagcggc gtcttctgcg gcgccgccac gcgcggcagg
      841 tagcggtaca gcgtcagctt gtcggcgcgg cagatgacct cgcgcgccgt ggcggcgagc
      901 tcgggagcac ctccggcgtg caggtggtcg agtccggctc cgaggcggcg gccgtagtcg
      961 agcagttccg cgtacggatc ggcgccggcg gaatcggcgg aatcgctggg cgtcatggct
     1021 gcgcgtcacc gcggccggcg aacgcgctgc gcagcgccgc cagctcgcgt tcgagctggc
     1081 gctggcgcgt gcgctgctcg tgcaggcgca gttcgagcgt gtcgatctcg gcgcgggtcg
     1141 gcaggtcgac gtgggcggcg agttcggcga cgagctgctg cagcgcgcgg cggcagtgca
     1201 gcatcgcgtg caccagccgc gcctgcgttt cggcgtagcg cggctcgcgg atcagcttcg
     1261 cccagacgtg ctcgctcgcc tcgacccagc ggtcgtacag cgcacgcacg ctggccggcg
     1321 gctcgcggcc gacctcggca cgcaggcgct tggcggcggc cgcggcggct tcatcgagca
     1381 gcgtccggta ggcggcgtcg gctgccgagt attcctgcca ggcgccggcg agttcgagcc
     1441 agagcgcgcg cgaggccgcc ggcgcgaggc cgggcagcgg caggccgcgg tgcagctcgg
     1501 cggccagccc cggcgagctg ccaccctgct gcagccacgg cagcagcgcc gcccaggtcg
     1561 ccggcagctg cccggcgagc gagcgccacg ccgcctgcca gccatcgtcg gcgctggcgc
     1621 cgaagggcag ggtctgcagc gagtcgatca gccggcgcca gtcggtggca tcggcatcgg
     1681 tctggccggt ggcggcgccg aggcgcagca gttcctcgac caggccgacg aagcgcaggc
     1741 cctgggcgag cgtgcgctcg aaggtgtcgc cggtgtcggg gttggcggcc cgccatgccg
     1801 cgagcgcggc atccggcgtc ggcgcccgtc cggcggcccc gctgcgcagc cattcctgca
     1861 gggcgtggca gtgcgcggcg agcagttcgc tcaggcggtc ggcggggccc tcaggggtgt
     1921 ccggcatcgg gggcgggtct cggcgggcgg atcagcgcgg cagtctggag gctttctcgt
     1981 actcgttcaa ttcgcgcagc cgctgcgcgc gctggcgcag ctgcgcgagc tgctggccgc
     2041 tggcgttcgg ggcgcgcagg ccgatctcgg cctgatcgaa cgcggcctgc acgtcgccgg
     2101 agagctcgta gaactggctc atcgccagat gcttgccggc gatgtcgccg gtctgctcgg
     2161 cggcgcgtgc gtagagctcc tgcagctcgg ccgactccgg gcggcggcgc agctgctcgt
     2221 cgagcaggcg gatggcgagg cgcgggtcgc cgcgcgtgac cagcgcctcg ccgtagctca
     2281 gcgtcagcac gtagtcgttg ccgtaggcgc ggcgcagggc ctcgtaggtc gaccaggcgc
     2341 gggcggtgtt gccggtgagc agcgccagcc gggcttcttc gagacgcagc gccagcgccg
     2401 cggcggccgg gcgcggcagc ttcgccagtt cgcgcgcggc gtcgtcgagg cgcccggcgc
     2461 gttcgagcgc gagcacgtag ccgtagcgat cggcctcgtc ggtgccgcct tcggcggcgc
     2521 ggcgctcgtg gtcgcgcacc gcctgcgccg gcgtggcgac cgacagcgcg gcgatgcgtg
     2581 cgcgcatgta gcggtaatag agttcgtcgc cgggcgtgcg gcccgtgggg cgggtgcgca
     2641 ggctgcggtt ctcggtgtcg gcggcgcggt tcagcgccag cgggtgcgtg cgcaggaact
     2701 ccggaatctg cgccgccgag ccggagaggt tttcgagccg cccgaagaag ctcgccatgc
     2761 cgttcggctc gaagccggcg cggatcagca ggtcggtgcc gacgcggtcg gcctcctgct
     2821 cgttggcgcg cgtgaagttg acgatgcgct gggcgctcgc cgccatcgag ccgaccgcga
     2881 ccgcctgacc gatctggcca ccaccgccac cggccgccgc cagggcgatg ccggccagca
     2941 tcgccgccat ggtcggcagg ccgatggtct ggcgatcggc gtaggcgcgg gtgatgtggt
     3001 gctgcacgac gtgcgcgatc tcgtgcgcga cgacgccggc gagttcgctc tcgctgcgcg
     3061 tctccagcat caggccggta tgcacgccga tgaagccgcc gggcagcgcg aaggcgttca
     3121 gctccgggtc gcgcacccag aagaagcgca gcgggctgcc cgcgccatcg gcgtgcagcg
     3181 cgagccggct gccgagcgag gcgaggtatt cgctcgccag cgggtcttcg agcacgaggc
     3241 cggcgtcctg gagcttcatc aggatgcgct ggccgatgcg ccgttcgtcc tgcgggccga
     3301 gcacctgctg ggacggatcg cccatgtcgg gcagcgcgat cgactgcgcg aggctttgcg
     3361 tgccgaggct cagcagcagc acgccgagga tcagggttcg catcgggagc agatcaacct
     3421 cgctgagggc agggcggcgg cccggcttgc cgcgccggcg agcgcgcgac cagaatgccg
     3481 cgcagccgca ggctgcgtcc gacgcgccag cgtatcagca cgtcaccacc gcagagaggc
     3541 gcgcacgccc catgaatacc gtcatcgacg ccgaactcga tgcggccggc ctgaactgtc
     3601 cgctgccgat cctcaagacc aagaaggcgc tggcggcgct ggccggcggg caggtgctgc
     3661 gcgtggtcgc gaccgatccg ggctcggtgc gcgacttcga agcctacacg cgccagaccg
     3721 gtcacgcgct gctggaaagc cgcgagcagg acgggcgctt cgtgttcgtg atccgcaagg
     3781 ccggcggctg agccgcgccc gcatggcccc gacatgaaaa tggtttcatt ctgctgcacc
     3841 tgcagcatcg gtgttacact ccctgcgaac cgcttcgtgg tcgtttcata tcaattcaat
     3901 cgcttggagg attgccgcaa tgcaacagtg ggtgccggag gaaaccttga agtcctggat
     3961 cgaggcgcgt gagcgtttct gggagcagta tcgtgcggcg ctgccgaccg ataccgatca
     4021 ggccgtcgac gcctggcgct cgctgcgcgc ccagcatctg gcgctctggg agaagctcgt
     4081 ccatgacacg ctggccggtc agacggcctg ggtcgatcgc ctgttcgcgc agctgtcgaa
     4141 cgcgccggct cctgagcgcg tcgtcgagtt ccgcggccag ttcgagcagg gcatccggca
     4201 gtggctggag acccaggacc ggctctgggg cgagctgttc acgctgatgc gcggcgaagc
     4261 cccggtggcg gccgacgtac cggtcgcccc ggaagccccg gcggccgaac cggccccggt
     4321 cgagccggtg tcccaggctc cggccgagcc ggtctacgag cagccggcgc cggtgtatga
     4381 gcagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntccca ggctccggcc
     4501 gagccggtct acgagcagcc ggcgccggtg tatgagcagc aggccccggt ctacgagcag
     4561 caggccccgg tgtacgagca gcaggcgccg gtgtacgagc agcaggcgcc ggtctacgag
     4621 cagcaggccc cggtgtacga gcagcaggcg ccggtgtatg agcagcaggc gccggtgtac
     4681 gagcagcaga cgccggtcta cgagccgatg ccgtccgagc cggtggccga tgacaccgtg
     4741 gcggatgccg gtgagtcgca gggcgagggc ggcgattcgg cgccgaagac gcgtcgccgt
     4801 cgcaccagca agtaagcacg tcacgcgcag tccccccctg gcggggcggc cggccgcaag
     4861 gtccgccgcc ccgtcgtttt tacggacccg gggcaggcat cagaccggca cgaactcgcc
     4921 gcgcatgtcg gccagcgccg ggaaactcgc gcggtagtcg agcagcgcat cgaggtggac
     4981 ctcggcgcgg atcgtgccgg ggccgctgcc ggcgtcggcg atgatctcgc cgcgcgggtc
     5041 aatgatcagg ctggcgccgc cgtagtgcag ccacgggtcg tcgccgcagc ggttgacgcc
     5101 gattacccag gcctggttct cgatcgcgcg tgccaccagc agcgcccgcc agtgcgcctc
     5161 gcgccgctgc ggccagttgg cgatgaccgt gtagagctgc gcgccgcgcc cggcggcgat
     5221 ccggtacagc tccgggaagc gcaggtcgta gcagatggtc ggcgcgaccc gcagcccggc
     5281 ccattcgtac agcacggggc cgtcgccctt cgcgtagtgg cggtcctcgt gcgcgaacga
     5341 gaacggatgg cacttgctgt agcgcgccag ctcgctgcca tccggcgccc aggtcaccga
     5401 ctggttcagg ccgcggccgt catcggcgcg cgtgacgagg ccggcgcaga tgaagatgcc
     5461 gaattcctgc gccagcgcgc tcaggaactg ctcgcccggg cgcgcctcgc cctcatcgat
     5521 cttcgcgacc ttcatgctga agccggtcat gcacatctcg ggcagcacgg ccagcgcgcc
     5581 cggcggcggg gcggcggcgg cgagcagctc ccgcacgtgt tcgtgattgg cgggcttgtc
     5641 ttcccagcgg atgtcggtct gcacggcgat gagctgcatg gcggggcacc ctgcgcgagg
     5701 tcacggtcag ggcggcaata tgccaccatt gccggcgccg cgatccgcgc tgctgacttg
     5761 ccgggagtgc cgacgatgac cgatctgccc acgattgcca cgctgctcgg cggtaccgtc
     5821 gccgtcggca cgccggtcac cgtgcagggc tgggtgcgca cgcgccgcga ttcgaaggcg
     5881 gggctgtcgt tcgtcgcggt gcatgacggc tcctgcttcg acgcgatcca gtgcgtggtg
     5941 ccggatacgc tcgcgaacta ccgcagcgag gtgctgcacc tcggcagcgg ctgctcggtg
     6001 cgcgtcaccg gcacgctggt ggcctcgggc ggcaagggtc agacggtcga ggtgcaggcc
     6061 gcggcgctcg aagtcatcgg gctggtcgag gaccccgaaa cctatccggt gcagccgaag
     6121 ccgcacacct tcgaatacct gcgcacggtc gcgcacctgc gtccgcgcac caacaccttc
     6181 ggcgcggtcg cgcgcgtgcg ccattgcctg gcgcaggcga tccaccgctt cttcagcgcg
     6241 cacggctact gctgggtcca cacgccgatc atcaccgcca gcgactgcga gggcgccggc
     6301 gagctgttcc gggtcagcac gctcgatttc gccaacctgc cgcgcacggc cgagggccgc
     6361 atcgactacg cgcaggactt cttcgggcgc gaggccttcc tgaccgtgtc cgggcagctg
     6421 aacgtcgagg cctactgcct ggcgctgtcg cgcgtatata ccttcggccc gaccttccgc
     6481 gccgagaact ccaacacctc gcgccacctc gccgagttct ggatggtcga gccggagatc
     6541 gcctttgccg atctcgccgc cgacgccgat ctggccgagg cgctgctgaa gtccgtgttc
     6601 cgcgccgtgc tcgacgagcg cggcgatgat ctcgcgttct tcgacgaccg catcctgaag
     6661 ggctgcatcg cgcgcctcga agcgctggtc gaacagccgt tcgcgcgcat cgactacagc
     6721 gaggcggtgc gcgtgctgga gggctgcggc gagaccttcc agttcccggt cacctgggga
     6781 gcggacctgc agtccgagca cgagcgcttt ctggccgagc gccatgtcgg cgggccggtc
     6841 gtggtcatga actacccgaa ggcgatcaag agcttctaca tgcgcctgaa cgacgacggg
     6901 cgcacggtcg cggcgatgga cgtgctcgcg cccggcatcg gcgagatcat cggcggcagc
     6961 cagcgcgagg agcgtctcga cgtgctcgat gcgcgcatgc gcgaatgcgg actggatccg
     7021 gccggctacg gctggtaccg ggatctgcgc cgctacggca cggtgccgca tgcgggcttc
     7081 ggactggggt tcgagcgcct ggtcagctac gtgaccggca tggcgaacgt gcgcgacgtg
     7141 atcccgtttc cgcgcacgcc cggcagcgcc gagttctgaa ccccgggcgg ggcgcagtca
     7201 gtcgtgcgag aagaacaggc tgccgctgcg ctgccagggc tcggcggcct tgcgccgggc
     7261 ggtgccggca ccgatgcgtc gggcgcgcca ttcggcaaac acggcaaccg tggcgctggg
     7321 cagcagaagt ccgatgatgg cgagcataga catgacaggc acctccccct ccaatgtccg
     7381 ctgtccgtgc ggatcggtta atcgatcaga aagcatgcgg gacagcgtaa cgaacgtggc
     7441 gggccatcgc tgtgatgcct tgtttcccgg gccgggcagg gcgcgggcgg ttctgcttat
     7501 tcggcagcga tggctggcgg agaaagtaaa agagggttgc gatgatcgca accctccaaa
     7561 ttggctcccc gagacggact cgaaccgccg acccagtgat taacagtcac ttgctctacc
     7621 gactgagcta tcggggaatg aattaaattg tcgtaaaccg gtgaatcact ttgccgacgt
     7681 gcagacattg caatgcagca cggcggtaca ggtaaattcg gtaattggct ccccgagacg
     7741 gactcgaacc gccgacccag tgattaacag tcacttgctc taccgactga gctatcgggg
     7801 aaccgttgcg agccgcgcat cctatagatg gccgccacgc gcgtcaaccg cgatgcagcg
     7861 gccgcccggg cccgggcggc cgctgcgcga cgctcagcgc aggacttccg cgatcgcggc
     7921 ggcgaccgtc ggcaggttgg tggtgttcag cgcggccacg cagatgcgcc cggtgccgac
     7981 cgcgtagatg ccgtgctcgg tcttcaggcg ctcgacctgg gcggcgctca ggcccgagta
     8041 cgagaacatg ccgcgctggc gctgcacgaa gccgtagtcg ccgggcacgc cggccgcggc
     8101 gaggccggcg accaggccgc tgcgcatcgc gcggatgcgc tcgcgcatct cgccgagttc
     8161 ctgctcccac agcgcgcgca gttcgtggct gccgagcacg gcggcgacga tcgaggcgcc
     8221 gtgcgtcggc gggttcgagt agttggtgcg gatcacgcgc ttgagctgcg agagcacgcg
     8281 cgccgattcg tcgcggctgc cggtgacgat cgacagcgca ccgacacgct cgccgtacag
     8341 cgagaacgac ttcgagaacg agctggcgac gaagaacggc aggccggatt cggcgaacag
     8401 gcgcaccgcg accgcgtcgg catcgatgcc gtcgccgaag ccctggtagg cgatgtcgag
     8461 gaagggcacg tgaccgcgct cggcgagcac ggccagcacc tgctgccact gcgcgggcgt
     8521 caggtcggcg ccggtcgggt tgtggcagca ggcgtgcagc acgacgatcg agttcgccgg
     8581 cagggcctgg aggtcggcga gcatgccggc gaggtcgacg ccgtggttcg gggcgtcgta
     8641 atagcggtag ctgccgacct cgaagccggc cgattcgaac agcgcgcggt ggttttccca
     8701 gctcggatcg gagatcgcga cgctggcgcc gggcagcagg cggcgcagga aatcggcgcc
     8761 gatcttcagc gcgccggtgc cgccgagcgc ctgcgcggtg acgacgcggc cttcggcgag
     8821 cagcggtgaa gcggtgccga acagcagttc ctgcaccagc ttgtcgtagg cggcgatgcc
     8881 ttcgatcggc aggtagccgc gcggcgcggc cgcggcgatg cgggccttct cggcctcctg
     8941 gacggcgcgc agcagcggga tgcggccggc gtcggtgaag tagacgccca cgcccagatt
     9001 gaccttggtg gcgcgcggat cggcgttgta ggcctcgttg agcccgagga tcgggtcgcg
     9061 cggggccatt tcgacggcag agaacaggct catgtcggca gctcggagct aacagggaga
     9121 aagggcgaag aaccggagga tcaacggctt gtcttgaacg ggccgcagcg ggccagtata
     9181 tccccttccc catgcgccgc gaagcaggcc gaggagccac gagcatgcag cgattcgaac
     9241 tcgtcaccga ctatcagccg gccggcgacc agccggcggc gatcgacgcg ctggtgcgcg
     9301 gcctggagga cgggctcgcg caccagacgc tgctcggcgt caccggcagc ggcaagacct
     9361 tcacgatggc cagcgtcatc gcccgcgtgc agcgtccggc gatgatcctc gcgcagaaca
     9421 agacgctggc cgcgcagctc tacggcgaga tgaaggcgtt cttcccgcac aacgccgtcg
     9481 agtacttcgt ctcgtactac gactactacc agcccgaggc ctatgtgccg gcctcggaca
     9541 ccttcatcga gaaggacgcc tcgatcaacg cccacatcga gcagatgcgg ctgtcggcga
     9601 ccaaggcgat cctggagcgc gccgacacga tcatcgtcgc gtcggtgtcc tcgatctacg
     9661 gcctcggcga ccccgagctg tacctgcaga tgatcctgca cctgtcgcgc ggcgagcgca
     9721 tcgaccagcg cgacatcctc aagcgcctca cggcgctgca gtacgtgcgc aacgacgccg
     9781 tgctcgagcg tggcagcttc cgcgtgcgcg gcgaggtcat cgacgtgcac ccggccgatt
     9841 ccgaaaccga ggccgtgcgc atcgagctgt tcgacgacga gatcgagcag ctgtcgtact
     9901 tcgatccgct gaccggcgtc gtcgcgcggc gcgtgccgcg cctgaccatc cacccgaaga
     9961 cgcattacgt gacgccgcgc gcgcgcatcc tcgaaaccgt cgatgccgtg aagctcgaac
    10021 tcgccgcgcg cgtcaccgaa cttgaacgcg ccgggcgcct gctcgaagcc cagcgcatcg
    10081 agcagcgcac gcgcttcgac ctggagatga tgctggagct cggctactgc agcggcatcg
    10141 agaactactc gcgctacctg tccggccgcc aggccggcga gccgccgccg acgctgttcg
    10201 attacctgcc gccgcacgcg ctgctgatca tcgacgagtc gcacgtgacg gtgccgcagc
    10261 tcggcggcat gtaccgcggc gaccgttcgc gcaaggagac gctggtcgag tacggcttcc
    10321 ggctgccgtc ggcgctcgac aaccggccgc tgcgcttcga cgagttcgag cggctggcgc
    10381 cgcagacgat cttcgtgtcg gcgacgcccg gcaactacga aggcgagcac gccggccagg
    10441 tggtcgagca gctcgtgcgc ccgaccgggc tcgtcgatcc gcaggtcgag gtgcgcccgg
    10501 cgcgcacgca ggtcgatgac ctgatgtccg agatcgtcgc gcgcgtgcgc ctcgacgagc
    10561 gcgtgctggt cacgacgctg accaagcgca tggccgagga cctcaccgaa tacctcgccg
    10621 agcacggcgt gcgcgtgcgc tatctgcacg ccgacatcga gacggtcgag cgcgtcgaga
    10681 tcatccgcga cctgcgcctc ggcgagttcg acgtgctcgt cggcatcaac ctgctgcgcg
    10741 agggcctcga catgcccgag gtgtcgctgg tggcgatcct cgatgccgac aaggaagggt
    10801 tcctgcgctc cgagcgctcg ctgatccaga ccatcggccg cgcggcgcgc catctgaacg
    10861 gccgggcgat cctgtacgcc gaccgcatca ccgactcgat gcgccgcgca ctcgacgaga
    10921 ccgggcgccg gcgcgtgaag caggaagccc acaacgccgc gcacggcatc gtgccgcgca
    10981 gcgtgcgcaa ggcggttgcc gacatcctcg aaggcggcat tcccggcgcc ggtcgcagcc
    11041 ggcgcgagcc ggcggccgag gccggactcg ccgaatcgcg cggcccgtat cgggtgctga
    11101 cgccggagca ggcggcgaag aagctgcgcg agctcgaagc gcggatgctg cgccacgcgc
    11161 gcgatctgga gttcgaggag gcggcgcgcc tgcgcgacga gatcgtgcgc atccgcgaga
    11221 ccaccttcgg cgccggtcag gcagcggcgg gctgagccgg aaaaaatccg cacggccggc
    11281 ttgcatcggc cgggggcgat ccgtaacatg cgcggcctgc aaattgcagg cgcgtagctc
    11341 agctggttag agcaccacct tgacatggtg ggggtcgttg gt
//
LOCUS       DAOQIE010000099        11315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2849_length_11315_cov_8.177087, whole genome shotgun sequence.
ACCESSION   DAOQIE010000099 DAOQIE010000000
VERSION     DAOQIE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_11315_cov_8.177087"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1290)
                     /locus_tag="PK144_07880"
     CDS             complement(10..1290)
                     /locus_tag="PK144_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF65683.1"
                     /translation="MVNPMRSPGAGGRRVLLIAFHYPPASESSGIQRTLKFSQYLPEH
                     GWTPAVLSAHPRVYDRVSSEQLAEVPAGMPVLRAFALDTARHLSIAGRYPRALALPDR
                     HVSWALGGIVQGMALIRRFRPDVLWSTYPIASAQLIGATLARLTGLPWVADLRDSMTE
                     DEYPRDPVQRASFLKLEKRVVTHAARVVFTTPGARAMYAARYPEVPPERWTVIENGFD
                     EENFTRAAGREPPPRSDRARVRLVHSGVLYPSERDPRPFFDALAALKARGVVDAARLC
                     VVLRYTGHDALHRSEIERRGIQDLIELAPPVPYEAALAEQLAADGLLLFQAANCNHQI
                     PAKLYEYLRAGRPILALTDAAGDTAATLRAAGIDSIVALDDAAAIEAALATFLDRIED
                     GSAVRIGRPQAERHGRRARTGELAALLDALVASR"
     gene            complement(1284..2144)
                     /locus_tag="PK144_07885"
     CDS             complement(1284..2144)
                     /locus_tag="PK144_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HNF65684.1"
                     /translation="MERCFAIDCAGERLPAILHPGTPGAARGVLVVVGGPQTRVGSHR
                     QFVLLARQLAAAGVPVLRFDYRGMGDADGEARSFEDVDADLAAACAAFRRESPGLGEI
                     VIWGLCDAASAALFHAWRAPQGIAGLVLLNPWVRTDTGEARAYIGHYYRRRLLSAAPW
                     KKMLRGEFDFRGSLRSAFGLLRRALARTPADTQAGGADDPTQGLALPARMAECLRRFR
                     GPVLLVLSGDDLTAAEFRDCVQQSAQWQALLAEARVTRRELAGANHTFSRRVWRDAVA
                     GWTLEWLRTW"
     gene            complement(2174..3004)
                     /locus_tag="PK144_07890"
     CDS             complement(2174..3004)
                     /locus_tag="PK144_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HNF65685.1"
                     /translation="MLPEFLPGRCARLLAVRHAPAPGVALRGAVLCFVPFAEEMNKSR
                     RMLTLAARRLAADGWGVLIVDPHGTGDSGGDFVDARWDIWQDDYAACARWLQARHPGR
                     LVLWGVRTGALLAAELAGSAALAVDTLLYWQPVTVGDLALTQFLRLRLAAGLMDGSKE
                     TAAGLRERLNAGESLEIAGYELHPALAAALERSRLLPPPAGVPVLWLELVSEAGRAPG
                     AVAQKLLTGWRKDGHELRCETVVGDSFWATQEIAVVPALVEATAAMLAGAALPAGAGA
                     "
     gene            complement(3030..3278)
                     /locus_tag="PK144_07895"
     CDS             complement(3030..3278)
                     /locus_tag="PK144_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF65686.1"
                     /translation="MLNFEETRALIGDVLQLGERTAEIHEDTLLLGSIPEFDSMAVVS
                     LITAIEDRFGIAIDDDELSADTFASAGSLHAFIAARVD"
     gene            complement(3467..3994)
                     /locus_tag="PK144_07900"
     CDS             complement(3467..3994)
                     /locus_tag="PK144_07900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNF65687.1"
                     /translation="MNGFHPAAGVSVRAVEAADHAAIATVVDSAFAAAAHADGTEAAL
                     VAELRAAGGIACEWVAVEGTAPVGHIAFSAVTLDGRDCNWFGLAPLAVAPAYQGRGIG
                     ARLVRTGLAALQARGAGGCVVLGEPAYYARFGFAALPVLVCAWPVPPQYFQALAWQPL
                     ACAGIVRYHPLFGSV"
     gene            complement(3991..4293)
                     /locus_tag="PK144_07905"
     CDS             complement(3991..4293)
                     /locus_tag="PK144_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65688.1"
                     /translation="MKIKNMMRYSGYGPCSGPALLSGCGDNVRFWAVGALDGLDAGRT
                     ARGAGRRPFCMAVRPFALIVPRCKPPAGSRRPIPTQAALRALSGERSAQAGRGAGA"
     gene            4366..4731
                     /locus_tag="PK144_07910"
     CDS             4366..4731
                     /locus_tag="PK144_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNF65689.1"
                     /translation="MSALASLPRTSVTRHPLLVLLLAAPLAIGAVGCGHGPATDAGVG
                     ALTGAAVGAGVGGRKGALVGGVVGAAAGVAIGQEQERRRAEAERARYYDDGYGRNYDR
                     SYNRGYQDGYRRDPYYDNY"
     gene            4848..5315
                     /locus_tag="PK144_07915"
     CDS             4848..5315
                     /locus_tag="PK144_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNF65690.1"
                     /translation="MPVLFDSALLDLLRNHARDSPRRRAHHPLHRSLDEPCQRLLVAI
                     EPDSYVPPHRHLQPEKSEALIVLRGRLACFTFDESGTCTQRTVLAPGDGIDVPPGEWH
                     AVLALDPGTVFFETKAGPYRPLDETERSAWAPGEQDDGAAAYREWLRGLCANH"
     gene            complement(5340..6014)
                     /locus_tag="PK144_07920"
     CDS             complement(5340..6014)
                     /locus_tag="PK144_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNF65691.1"
                     /translation="MSFRIPDSLSLRRMPRGLAALLLAAALAVPVQARADVAETVLPA
                     ACTRDCAAPWGETLGGSTTTPAYSNCSAACFVPLPNREQGIFTGIRWQCVEYARRWWL
                     LNRGVFFGDVDVAADLWLKIPAVTRASDGSTQPLENHDNGDSAPPKVGDLLVYGREYL
                     RTGHLAVVVAVDAGAGRIDVAEQNFLNRPWPAADHARSLDLLERKGRWWVLDPYLLGW
                     KRIAGE"
     gene            complement(6109..6900)
                     /locus_tag="PK144_07925"
     CDS             complement(6109..6900)
                     /locus_tag="PK144_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNF65692.1"
                     /translation="MSLSIHIPKILGAAASAALLGMLLAPSGSHAEDHPAGPAHWSYE
                     GHDGPEHWATLDPKYATCSGGHSQSPINISDVQQSGLPPITFDYRKAAVDDVNNGHTV
                     QFQLANGSAITVAGRRYELLQAHFHEPSEHTVNGKSYPAEVHFVHRDANGALAVVGVL
                     IAEGTANRTLSTMWAYLPKAAGETQQGGKSSGFDPAGLLPKKREYYTYSGSLTTPPCT
                     EGVTWLLMQQPITASKAQIRQFGELFAMHPTNRPVQPLNGRVVAR"
     gene            complement(7068..7820)
                     /locus_tag="PK144_07930"
     CDS             complement(7068..7820)
                     /locus_tag="PK144_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF65693.1"
                     /translation="MNPKLSSPAAPKPDSGPDAEDGVLPAALEPLPGVDPGSSVVREP
                     WRRYALYGLAALALWGALWVLMLASGAADAVDARIGGFDPAAPLAGMMRDLTSFGSAT
                     FITLFALTGWIVLMARRRTRDALQVALAAAGSGLWVETLKPLYARARPDALPALTEWF
                     SASFPSGHTLMSAALYPTLAVVFGATLKPAVRQAWFAAALALVVTVALSRVYLRVHFP
                     TDVIGGSVFGLLWAWVAHHGLRALLPYRAPMG"
     gene            7877..8839
                     /locus_tag="PK144_07935"
     CDS             7877..8839
                     /locus_tag="PK144_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNF65694.1"
                     /translation="MDTLPISLILNPGSGSRRDPALAQSILDTLSAGGRQVEWLKPRR
                     GAEIPAMAAKAMANGPRLMVAAGGDGTVNAVAGAVLAHPGSELGVIPLGTFNYFARGL
                     RLPEDPLAAAGIVRDGQTTAISVGSANGRVFLVHASFGLYRSVIEARERHKNLIGRHR
                     LVAAISGLITALRPHPHYRLKLGIGDEAMRRVSSMVYFGANPLQNAELGVGGADTPES
                     GLAVFVLRPMTRWQVLVTTCHALFGHVADSESIEVFHAQEVTVERHRKRINLVLDGEI
                     FHEQSPLVVRWLPDALPVRVAPVIEDDAQALQDDAPPATMEPAA"
     gene            8836..9657
                     /locus_tag="PK144_07940"
     CDS             8836..9657
                     /locus_tag="PK144_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF65695.1"
                     /translation="MRTIAHVSDLHFGRIDPAASAALGAALRSLKPDLVIVSGDLTQR
                     ARSEQFREAAEFLAGLATPVLAVPGNHDVPLWHALARFLWPYHGYRRHIAVELEPVHE
                     DAELLVLGLNSARSLAFYGGRLNSEQVGRAVERLRHAGRERLKIVVSHHPLSAPLTAG
                     PEAESVGRGARMYRRLVDAGGEVFLAGHFHHTAHELAEPLATSRRRALLVHAGTATST
                     RRRASEGNGFNLLRYAGGHLSVVHYEHRSQRSTDFVPVTSRDYHRGDYGWEPLAH"
     gene            complement(9713..10612)
                     /locus_tag="PK144_07945"
     CDS             complement(9713..10612)
                     /locus_tag="PK144_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNF65696.1"
                     /translation="MTAFGAADWPYRLGCPVWASKTWNGGFYTPDAGPGDWLAQYSSV
                     FGTVEGNASFYGLPAPATVARWAQDAAPGFRFCFKVPRAITHEARLIDCRAATAEFLD
                     RLAPLAARDALGPLLLQLPPDYGPDGLARLLDWLDALPAGRTCAVEMRHPCFYDRSPA
                     ERALNRALHERGIDRATMDTRGLFSATDADPATREARRKKPRLPARALALGPRPFVRY
                     VGHPVLARNGHWLAQWAGQFATWITEGREPWFFAHMPDDRDAPALARAFHEELRSRIA
                     SLPALPPWPAGRLPQQSGLFGGN"
     gene            complement(10609..>11315)
                     /locus_tag="PK144_07950"
     CDS             complement(10609..>11315)
                     /locus_tag="PK144_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65697.1"
                     /translation="EGFTDPSFTYPLVRSGGLLVGGVASSIDPLPARIADREAELLAA
                     AIRSERPGLPVQTAALADPRFAAAGQAARLQALARGPLPPETYAALAPLRKAGRFVVY
                     ARLENDAVRQEHDIREHDLPDPMREARDRKGKRILVPLYDRAELVESFTTRRVVDVRY
                     TVLDLDARREVWSGRIEKSAEASNRVTLAEGESGSFGYVAPAVPPPGAFPPPPVAGRL
                     IEAAHRGFAENLPEAP"
BASE COUNT         1676 a         4091 c         4033 g         1515 t
ORIGIN      
        1 cggcgcggct cagcgcgatg caacgagcgc gtcgagcagc gcggcgagtt cgccggtgcg
       61 ggcacggcgg ccgtggcgct cggcctgcgg gcgtccgatg cgcacggcgc tgccgtcctc
      121 gatacgatcg aggaaggtcg caagcgcggc ctcgatcgca gcggcatcgt cgagcgcgac
      181 gatgctgtcg atgccggcgg cgcgcagcgt cgcggcggta tcgccggcgg cgtcggtcag
      241 cgccaggatc ggccggccgg cgcgcaggta ttcgtagagc ttggccggaa tctggtgatt
      301 gcagttggcg gcctggaaca gcagcaggcc gtcggccgcg agctgttcgg cgagcgcggc
      361 ctcgtagggc acgggcggcg ccagttcgat caggtcctgg atgccgcggc gctcgatctc
      421 gctgcggtgc agcgcatcgt ggccggtgta gcgcagcacc acgcagaggc gcgcggcatc
      481 gacgacgccg cgcgccttca gcgcggcgag cgcatcgaag aacggccgcg gatcgcgctc
      541 gctcgggtac agcacgccgc tgtgcacgag ccgcacgcgc gcccggtcgc tgcgcggcgg
      601 cggctcgcgg ccggcggcgc gcgtgaagtt ctcctcgtcg aagccgttct cgatgaccgt
      661 ccagcgttcg ggcggcacct ccggatagcg cgccgcgtac atcgcgcgtg cgcccggcgt
      721 cgtgaacacc acgcgtgcgg cgtgcgtgac gacgcgcttc tcgagcttca ggaagctcgc
      781 gcgctgcacc ggatcgcgcg ggtactcgtc ctcggtcatc gaatcgcgca ggtcggcgac
      841 ccacggcagg ccggtcaggc gcgccagcgt cgcgccgatc agctgcgcgc tggcgatcgg
      901 gtaggtcgac cacagcacgt cgggccggaa gcgccggatc agcgccatcc cctgcacgat
      961 gccgccgagc gcccagctca cgtgccggtc gggcagcgcc agcgcgcgcg gatagcggcc
     1021 ggcgatcgac aggtggcgcg cggtgtcgag cgcgaaggcc cgcagcaccg gcatgccggc
     1081 ggggacctcg gcgagctgct cgctgctgac gcggtcgtag acgcgcggat gcgcgctcag
     1141 caccgccggc gtccagccat gctccggcag gtactgcgag aacttcagcg tgcgctggat
     1201 gccgctgctc tcgctggccg gcggatagtg gaaggcgatc agcagcacgc gccgcccgcc
     1261 cgctccggga gaccgcatcg gattcaccat gtgcgcagcc actccagcgt ccagccggcc
     1321 acggcatcac gccacacgcg ccgggaaaag gtgtgattgg ccccggccag ctcgcgccgc
     1381 gtgacgcgcg cctcggcgag cagcgcctgc cactgcgccg actgctgcac gcagtcgcgg
     1441 aattcggccg cggtcaggtc gtcgccggac agcaccagca gcaccggccc gcggaagcgg
     1501 cgcaggcact cggccatgcg cgccggcagc gccaggccct gcgtcgggtc gtccgcaccg
     1561 cccgcctgcg tgtccgccgg cgtacgcgcc agcgcccggc gcagcagccc gaaggccgag
     1621 cgcagcgagc cgcggaagtc gaactcgccg cgcagcatct tcttccacgg cgcggcgctc
     1681 agcagccggc ggcggtagta atggccgatg taggcccgcg cctcgccggt atcggtgcgc
     1741 acccagggat tcagcagcac cagcccggcg ataccctgcg gggcgcgcca ggcgtgaaag
     1801 agcgccgccg aggcggcatc gcagagcccc cagatcacga tctcgccgag tccgggtgat
     1861 tcacggcgaa aggccgcgca ggcggcggcg agatcggcat cgacatcctc gaagctgcgc
     1921 gcctcgccgt cggcatcgcc catgccgcgg taatcgaagc gcagcaccgg cacgccggcc
     1981 gccgccagct gccgggcaag cagcacgaac tggcgatgac tgccgacgcg ggtctgcggc
     2041 ccgccgacca cgaccagcac gccgcgcgcc gcaccgggcg tgccggggtg caggatcgcc
     2101 ggcagccgct cgccggcgca gtcgatggcg aagcagcgct ccagcgcctc ggcagggacc
     2161 ggggccgcgg tgctcacgct ccggcaccgg ccggcagcgc ggcgccggcg agcatggcgg
     2221 ccgtcgcctc gaccagcgcc ggcacgaccg cgatttcctg cgttgcccag aagctgtcgc
     2281 cgacgacggt ctcgcagcgc agctcgtggc cgtccttgcg ccagcccgtg agcagcttct
     2341 gggcgacggc ccccggcgcc cggccggctt cggacacgag ttcgagccac agcaccggca
     2401 cgccggccgg cggtggcagc aggcggctgc gttcgagcgc ggcggcgagc gccgggtgca
     2461 gttcgtagcc ggcgatctcc agcgactcgc cggcgttcag gcgctcgcgc aggccggccg
     2521 cggtttcctt gctgccgtcc atcaggcccg cggccagacg caggcgcagg aactgcgtca
     2581 gcgccagatc accgaccgtc accggctgcc agtacagcag cgtgtcgacc gccagcgcgg
     2641 cgctgcccgc aagctccgca gcgagcagcg cgccggtgcg cacgccccac agcaccaggc
     2701 ggccggggtg gcgcgcctgc agccatcgcg cgcaggcggc gtagtcgtcc tgccagatat
     2761 cccagcgcgc gtcgacgaaa tcgcctccgc tgtcgccggt cccgtgggga tcgacgatca
     2821 gcacgcccca gccgtcggcc gccagccgcc gcgcggccag cgtcagcatg cgccgggact
     2881 tgttcatctc ctcggcgaag ggcacgaaac acagcacggc accgcgcagc gccacgccgg
     2941 gcgccggtgc atggcgcacg gcgagcagcc gtgcgcagcg accgggcagg aactcgggca
     3001 gcatcgggga agggaaggcc ggcgccgctt cagtcgacgc gggcggcgat gaaggcgtgc
     3061 aggctgccgg cactcgcgaa ggtatcggcg ctcagctcgt cgtcgtcgat cgcgatgccg
     3121 aagcggtcct cgatcgcggt gatcaggctg accacggcca tcgagtcgaa ttcggggatg
     3181 ctgccgagca gcagcgtgtc ttcgtggatt tcggcggtac gctcgccgag ctgcagcacg
     3241 tcgccgatga gggcgcgggt ttcttcgaag ttcagcatgg ggaccgcttc cgggctcggg
     3301 atcggggctg cctgtggcgc gacgacccgg atcgggccgg tgacgcacag ggcgcggaaa
     3361 gataccggag cggccctgcg cgcgccagtc gcacgcttgt gaacagcggc atcggccagg
     3421 attcccggaa gtttccgcaa gattgcgcca tcacgatgcc gaaggctcag accgatccga
     3481 acagcgggtg ataccgcacg atgccggcac atgccagcgg ctgccaggcg agtgcctgga
     3541 aatattgcgg cggcaccggc caggcgcaga ccagcaccgg cagcgcggcg aagccgaagc
     3601 gagcgtaata ggccggttcg ccgagcacga cgcagccgcc ggcgccgcgc gcctgcagcg
     3661 ccgcaagccc cgtgcgcacc agccgcgccc cgatgccgcg cccctgatac gccggtgcca
     3721 ccgccagcgg cgccagcccg aaccagttgc agtcgcgacc atcgagcgtc acggcactga
     3781 aggcgatgtg gccgaccggc gccgtgccct cgacggccac ccactcgcag gcgatgcccc
     3841 cggcggcccg cagctcggcc accagcgccg cctcggtgcc atcggcatgc gccgcagcgg
     3901 cgaaggcgga atcgacgacg gtggcgatcg cggcgtgatc ggcagcctcg acggcgcgca
     3961 ccgaaacccc tgcagccgga tggaagccgt tcatgcgccg gccccgcgac cggcctgcgc
     4021 gctgcgctcg cccgagagcg cacgcaaggc tgcctgcgtc gggatcggac gacggctgcc
     4081 ggcaggcggc ttgcagcgcg gcacgatgag cgcaaacggg cggacggcca tgcagaacgg
     4141 cctgcggccc gctccccggg ccgtcctgcc ggcatccagc ccatcgagcg caccaaccgc
     4201 ccaaaaccgg acgttgtcgc cgcaccccga cagcagcgcc ggtcccgagc agggtccgta
     4261 tccggaataa cgcatcatgt ttttgatttt catgaataac accggcatgg cactacaagt
     4321 gctctgtctc cctcacccgt tcccggcctg tcaggagccg ccatcatgtc cgccctcgcg
     4381 tcgctgcccc gcacgtccgt cacccgtcat ccgctgctgg tgctgctgct cgccgccccg
     4441 ctcgccatcg gggcagtcgg ctgcggccac ggcccggcca ccgatgccgg cgtcggtgcg
     4501 ctgaccggtg cagcagtcgg tgccggcgtc ggcggcagaa agggtgccct cgtcggtggc
     4561 gtggtcggag ccgctgccgg tgtcgcgatc ggtcaggaac aggaacgccg ccgggccgag
     4621 gccgaacgtg cgcgctatta cgacgacggc tacggacgca actatgaccg cagctacaac
     4681 cgcggctatc aggacggcta ccgtcgcgat ccgtactacg acaactactg agcccgatgt
     4741 gaccgggctc cggtttccgg cggctgcgcc cggcacatcc gcgtgagtgg acggcccgca
     4801 gccggtaggc tgccggacct gcccttcccg atgccggacc gtccgcgatg cccgtgctgt
     4861 tcgattccgc cctgctggac ctgctgcgca accatgcacg ggacagcccg cgccgccgtg
     4921 cgcatcaccc gctgcaccgc agcctcgatg aaccctgcca gcgcctgctg gttgcgatcg
     4981 agccggattc ctacgtcccg ccgcaccgcc atctgcagcc cgaaaaatcc gaagccctga
     5041 tcgtgctgcg cggccggctt gcctgtttca cgttcgacga atccggcacc tgcacgcagc
     5101 gcacggtgct ggcgcccggg gatggcatcg acgtgccgcc cggtgaatgg catgcggtgc
     5161 tggcgctcga tccgggcacg gtgttcttcg aaaccaaggc cggtccgtac cgacccctgg
     5221 acgagactga acggtccgca tgggcgccgg gcgagcagga cgacggtgct gcggcgtacc
     5281 gtgaatggct gcgaggtctc tgcgcgaacc actgaaccac gcactgtcgc atcgcccgct
     5341 cattcgccgg cgatgcgctt ccagccgagc agatacggat cgagcaccca ccagcggccc
     5401 ttgcgttcga gcagatcgag gctgcgcgca tgatcggcgg ccggccacgg ccggttcagg
     5461 aagttctgtt cggcaacatc gatgcgcccg gccccggcat cgacggcgac gacgaccgcg
     5521 agatggccgg tacgcaggta ctcgcggccg tagaccagca ggtctccgac cttcggcggg
     5581 gcgctgtcgc cgttgtcgtg gttttcgagc ggctgcgtgg agccgtcact ggcgcgcgtg
     5641 accgcaggga tcttcagcca cagatcggcg gcgacatcga catccccgaa gaacacgccg
     5701 cggttcagca gccaccagcg ccgtgcgtac tcgacgcact gccagcggat gccggtgaat
     5761 atcccctgct cgcgattcgg cagcggcacg aagcaggcgg cgctgcagtt ggaataggcc
     5821 ggcgtggtcg tgctgccgcc gagggtttcg ccccagggcg cggcacagtc gcgcgtgcag
     5881 gccgccggca ggacggtttc ggcgacatcg gcccgcgcct gcacgggcac ggcgagcgcg
     5941 gcggcgagca gcagcgcggc cagcccgcgc ggcatgcgtc gcaggctcag ggaatcggga
     6001 atgcggaagg acatggcggc aggcccggca cggcggcgaa ggtccgtgcg gtatgcccgc
     6061 cgccggccgg cgctgtcaac gcgcaccggc cgggaggccg gcaaggcctc agcgggcgac
     6121 gacgcgcccg ttcagcggct gtaccggacg gttggtcggg tgcatcgcga acagctcgcc
     6181 gaactgcctg atctgcgcct tcgacgcggt gatcggctgc tgcatcagca gccaggtcac
     6241 gccttcggtg cacggcggcg tcgtcagcga gccggagtag gtgtagtact cgcgcttctt
     6301 cggcagcagg ccggccggat cgaagccgct cgacttgccg ccctgctgcg tctcgccggc
     6361 cgccttcggc aggtaggccc acatcgtcga cagcgtgcgg ttcgccgtgc cttcggcgat
     6421 cagcacgccg accacggcca gcgcgccgtt ggcgtcgcgg tgcacgaaat gcacctcggc
     6481 cggataggac ttgccgttga cggtgtgctc gctcggctcg tggaagtggg cctgcagcag
     6541 ctcgtagcga cggccggcga cggtgatcgc gctgccgttg gcgagctgga actgcacggt
     6601 gtggccgttg ttcacgtcat cgaccgccgc cttgcggtag tcgaaggtga tcggcggcag
     6661 gccggactgc tgcacgtcgc tgatgttgat cggcgactgg ctgtgaccgc ccgagcaggt
     6721 cgcgtacttc ggatcgagcg tcgcccagtg ttcggggccg tcgtggcctt cgtagctcca
     6781 gtgcgccgga ccggccggat gatcctcggc gtggctgccg ctcggtgcga gcagcatgcc
     6841 cagcagcgcc gcggatgccg ctgcaccgag gattttcgga atatggatcg acaggctcat
     6901 gtttgactcc ccttcctcgt tttatgcggg atgcccgtgc gcgacgggcg tcgcatagtc
     6961 cgcggggagc ggtcgggaaa caaccggaat ttccggcttt taccggatgt tgcggattcg
     7021 tgccgatgct gcaccgcaca ctggccggcc ctgctgcggc ctgcgcctca gcccatcggc
     7081 gcgcggtacg gcagcagcgc ccgcaggccg tgatgcgcga cccaggccca cagcagcccg
     7141 aacacgctgc cgccgatcac gtcggtcgga aaatgcacgc gcaggtagac gcgcgacagc
     7201 gccacggtga cgaccagcgc cagcgctgcg gcgaaccatg cctggcgcac ggccggcttc
     7261 agcgtcgcgc cgaacacgac ggcgagcgtc ggatacagcg ccgcgctcat cagcgtgtgc
     7321 ccgctcggga acgaggcgct gaaccattcg gtcagcgccg gcagcgcatc ggggcgcgca
     7381 cgcgcataca ggggcttgag cgtctcgacc cacagcccgc tgccggccgc ggccagcgcc
     7441 acctgcagcg catcgcgggt gcggcgccgg gccatcagca cgatccagcc ggtgagcgcg
     7501 aacagcgtga tgaaggtcgc cgaaccgaag gaggtcaggt cgcgcatcat gccggccagc
     7561 ggcgcagccg gatcgaagcc gccgatgcgg gcatcgacgg catcggcagc accgcttgcg
     7621 agcatcagca cccacagcgc gccccagagc gccagcgcgg cgagaccgta cagcgcgtag
     7681 cgccgccacg gctcgcgcac gacgctgctg ccggggtcca cgcccggcag cggttcgagc
     7741 gcggccggca gcacaccgtc ctcggcatcg ggaccggaat cgggcttcgg ggcggcgggt
     7801 gaggacagct tcggattcat cgtctggact ccttcgggcc ggaacttgct gcgggcatcg
     7861 gctatcactg cccctcatgg ataccttacc catcagcctg atcctgaacc ccggctccgg
     7921 ttcccgccgt gacccggccc tggcgcagtc catcctcgac acgctctcgg ccggcggccg
     7981 gcaggtcgaa tggctcaagc cccggcgggg agccgagatc cccgccatgg cggcgaaggc
     8041 gatggcgaac ggaccacggc tgatggtggc cgccggtggc gatggcaccg tcaacgccgt
     8101 tgccggcgcg gtgctcgcgc atccgggcag cgaactcggc gtgatcccgc tcggcacctt
     8161 caactacttc gcacgcgggc tgcgcctgcc cgaggatccg cttgccgcag cgggcatcgt
     8221 gcgcgacggt caaaccactg cgatcagcgt cggcagcgcc aacggccggg tgtttctggt
     8281 gcatgcgagc ttcggactgt accgctcggt gatcgaggcg cgcgagcggc acaagaacct
     8341 gatcggccgt caccggctgg tcgcggcgat ctcgggcctg atcaccgcgc tgcgcccgca
     8401 tccgcactac cggctgaagc tcggcatcgg cgacgaggcg atgcgccggg tcagctcgat
     8461 ggtctacttc ggcgccaacc cgctgcagaa cgccgaactc ggcgtcggcg gcgccgacac
     8521 gccggagtcg ggtctggccg tgttcgtgct gcgaccgatg acgcgctggc aggtgctggt
     8581 gacgacctgc cacgcgctgt tcggtcatgt cgccgacagt gagtcgatcg aggtcttcca
     8641 cgcgcaggaa gtcacggtcg aacgtcaccg gaaacgcatc aacctcgtgc tcgacggcga
     8701 gatcttccac gagcaatcgc cgctggtggt gcgctggctg cccgatgcgc tgccggtgcg
     8761 cgtcgccccc gtcatcgaag acgacgcaca ggctctgcag gacgacgcac cgccggcgac
     8821 catggagccg gcggcatgag gacgatcgcg cacgtttcgg acctgcactt cggacgcatc
     8881 gacccggccg catccgcggc cctcggtgcg gcgctgcgca gtctgaaacc ggatctggtc
     8941 atcgtctccg gcgacctgac ccagcgtgcg cgcagcgaac agttccgcga ggccgccgag
     9001 ttcctggccg gcctcgccac gccggtgctg gccgtgcccg gcaaccatga cgtgccgctc
     9061 tggcacgcgc tggcgcgctt cctgtggccg taccacggct accggcggca catcgccgtc
     9121 gaactcgaac cggtgcatga ggatgccgaa ctgctggtgc tcggcctcaa cagcgcgcgc
     9181 tcgctggcct tctacggcgg acggctgaac agcgagcagg tcgggcgcgc cgtcgagcgc
     9241 ctgcgtcacg ccggtcgcga gcggctgaag atcgtcgtca gccatcaccc gctgtcggca
     9301 ccgctcacgg ccggtcccga ggccgaatcg gtcggacgtg gcgcgcgcat gtaccggcgg
     9361 ctggtcgatg ccggcggcga ggtgttcctg gccgggcatt tccatcacac cgcgcacgaa
     9421 ctcgccgaac cgctcgccac cagccgccgc cgggcgctgc tcgtgcatgc cggcaccgcg
     9481 acctcgacgc gccggcgcgc gagcgaaggc aacggcttca acctgctgcg ctacgccggc
     9541 ggtcacctga gcgtcgtgca ttacgaacac cgctcgcagc gcagcaccga cttcgtgccg
     9601 gtcacgagcc gcgactatca ccgcggtgac tacggctggg agccgctcgc gcactgacgg
     9661 cgtcgtgccc gcggcccgtc ccggcaaaac ccgggcaagg aatgtccggt tttcaattcc
     9721 ccccgaacag ccccgactgc tgcggcaggc gcccggccgg ccacggcggc aaggccggca
     9781 gcgacgcaat ccgcgagcgc agctcctcat gaaaggcccg cgccagcgcc ggggcatcgc
     9841 gatcatccgg catgtgcgca aagaaccacg gctcgcggcc ttccgtgatc caggtcgcga
     9901 actgcccggc ccactgcgcg agccagtgcc cgttgcgggc gagcacggga tgcccgacgt
     9961 agcgcacgaa cggccgcggt ccgagggcga gcgcgcgggc cggcagccgc ggtttcttgc
    10021 gccgggcctc gcgcgtcgcg gggtccgcgt ccgtggcgct gaagaggccg cgcgtgtcca
    10081 tggtcgcgcg gtcgatgccg cgctcgtgca gcgcgcggtt cagcgcccgc tcggcggggc
    10141 tgcggtcgta gaagcacgga tggcgcatct cgaccgcgca cgtccgcccg gccggcagcg
    10201 catccagcca gtcgagcagg cgcgcgagcc cgtcggggcc gtagtcgggc ggcagctgca
    10261 gcagcagcgg cccgagcgcg tcgcgggccg cgagcggcgc gagacgatcg aggaattccg
    10321 cggtcgcagc ccggcagtcg atgagccttg cctcgtgcgt gatcgcgcgc ggcaccttga
    10381 agcagaagcg gaagcccggc gcagcgtcct gcgcccagcg cgcgacggtc gccggcgccg
    10441 gcaggccgta gaagctggcg ttgccctcga ccgtgccgaa caccgacgag tactgcgcga
    10501 gccagtctcc cggcccggca tccggcgtgt agaagccgcc gttccaggtc ttcgaggccc
    10561 agaccgggca gccgagccgg tacggccagt cagcggcccc gaacgccgtc acggcgcctc
    10621 cggcaggttc tcggcaaagc cgcggtgcgc ggcctcgatc agccggccgg ccaccggcgg
    10681 cggcgggaag gcgcccggcg gcggcacggc gggcgcgacg tagccgaagc tgcccgattc
    10741 gccttcggcg agcgtgacgc ggttcgaggc ttcggcggac ttttcgatgc gcccgctcca
    10801 gacctcgcgc cgggcatcga ggtcgagcac cgtgtagcgc acgtcgacga cgcgccgcgt
    10861 cgtgaacgat tcgaccagct cggcgcggtc gtacagcggc accaggatgc gcttgccctt
    10921 gcgatcgcgc gcctcgcgca tcggatcggg caggtcgtgc tcgcggatgt cgtgctcctg
    10981 gcgcaccgca tcgttttcga ggcgcgcata gaccacgaag cgcccggcct tgcgcagcgg
    11041 cgccagcgcc gcataggtct cgggcggcag cggcccgcgc gccagcgcct gcagccgcgc
    11101 ggcctggccg gcagcggcga agcgcggatc ggccagcgcg gccgtctgca ccggcaggcc
    11161 ggggcgctcg ctgcggatcg ccgcggccag cagttcggcc tcgcgatcgg cgatgcgcgc
    11221 cggcagcgga tcgatcgacg aggcgacgcc gccgaccagc aggccgccgc tgcgcacgag
    11281 cgggtaggtg aaggacggat cggtgaagcc ttcgt
//
LOCUS       DAOQIE010000100        11242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2873_length_11242_cov_7.420220, whole genome shotgun sequence.
ACCESSION   DAOQIE010000100 DAOQIE010000000
VERSION     DAOQIE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2873_length_11242_cov_7.420220"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..653)
                     /locus_tag="PK144_07955"
     CDS             complement(102..653)
                     /locus_tag="PK144_07955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF65698.1"
                     /translation="MKFCSSCSAPVIVQIPEGDRLPRHVCIECGTIHYQNPRIVAGCI
                     PEWQGRVLLCRRAIEPRRGFWTLPAGFMENGETTDAAAARETFEEACARVEILGLYTL
                     YSLPHISQVHLLYRARLLDTEFAAGEESLEVGLYDEARIPWDELAFQTVRETLRHYFA
                     DRSRGEFPVHTGEIGPAPWQRRE"
     gene            1039..1945
                     /locus_tag="PK144_07960"
                     /pseudo
     CDS             1039..1945
                     /locus_tag="PK144_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010451277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(2064..2981)
                     /locus_tag="PK144_07965"
     CDS             complement(2064..2981)
                     /locus_tag="PK144_07965"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNF65699.1"
                     /translation="MDRTLETRLRQAGALASLPNVAMRVVALAHDPDVEAEPLLDEIQ
                     HDPPMTARVLRAANSVLYNHPRRITNLRQALVVMGTKGITHVALGFSLRGLMQPAGTS
                     PDFWRRSVYAALVGRELAHRLAPLAVEDVFVAALLQDLGILALEQALPADYARIVAGC
                     DGHAARAAAERAALGTDHAEVGAWLLGQWGLPELLIEAVARSHDPRGVAPARRTLVHC
                     VAASGVIADLWLDADHEAATERAWLAGDVWLGLDTEVLSGVLETVDRRLPDMMALFDL
                     EAVDPAQLDAVLAAARELLPHYAVSHGGR"
     gene            complement(3063..4019)
                     /locus_tag="PK144_07970"
     CDS             complement(3063..4019)
                     /locus_tag="PK144_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNF65700.1"
                     /translation="MNPLTWYDAPVRALLGGVFVLLVIASLIGRGLARRPRDGAGRAT
                     IENLNARIRAWWLMCALVAGALAGGLSGAVLLFGAISFLALREFVTLTPTRRADHEPL
                     FWVFFVFLPLQYWLVGTGWYGMFTILIPVYGFLFLPLRSALNADAQGFLARTARTQWA
                     LMVCVYCVSHAPALLTLELPGFDGSRVALLLWLLLVVQLSDVLQYVWGKLAGRRPIVP
                     RLSPNKTVEGFAGGIGSASLIGMLLAPVTPFTHGQAFGIALLVCLLGFAGGLVMSAIK
                     RDAGIKDFGAMIEGHGGVLDRIDSLCFSAPVFFHVVRYFWSP"
     gene            complement(4030..4671)
                     /locus_tag="PK144_07975"
     CDS             complement(4030..4671)
                     /locus_tag="PK144_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF65701.1"
                     /translation="MRRLARRGAALLLVGLCRGLTGVRALWLGSLPAAGARVYFANHA
                     SHLDGLVIWAALPPALRTRTHPVAARDYWDATPLRRWLALQVFGAVLIDRQPATAAGE
                     ADAARADVLAPLLAVLERGEALILFPEGTRGDGETVQPFRSGLWQLAQRCPRAELIPV
                     HLENLNRVLPKGSMLPVPLLCAARFGARFLPVDGETRADYLERARQAVLALAR"
     gene            complement(4671..5066)
                     /locus_tag="PK144_07980"
     CDS             complement(4671..5066)
                     /locus_tag="PK144_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65702.1"
                     /translation="MNPMKRMLPALLLVAVTPLAGAGALDGEYRGRADGERHLDLSEH
                     DDGQVSVTMSLDIPRTEGGRCRGEFVAHGLRDGRTITVEPATGKEACRIVIGVQAGQA
                     SISEQGEGCATLRDADCSFSGTLDRIRAR"
     gene            complement(5096..5683)
                     /locus_tag="PK144_07985"
     CDS             complement(5096..5683)
                     /locus_tag="PK144_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNF65703.1"
                     /translation="MNWLAHFYLAGPDPHARVGAMLGDFHRGPVETLADARWRAAVRE
                     HRAIDVWTDAHPLVCASRARLPAPLRRYGGIIIDVAYDHCLARDWANWSAQSLPAFVG
                     AVHAALRADYDRLPPRMQRSIDHLLQGRTLLSYREPAGVARALAGIATRLSRPSPLAA
                     AGPAVTALLPDWAGDFAVFFPELVAFVANRTDAGL"
     gene            complement(5702..7678)
                     /locus_tag="PK144_07990"
     CDS             complement(5702..7678)
                     /locus_tag="PK144_07990"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNF65704.1"
                     /translation="MRPEAWTRPAPGARLYGLPGLALLCAMAPHVTVVPLWLAAVFIA
                     TGLWRLGAEARDWALPNRWLLAAATVALCGAIGWQYRTLFGREAGVALLYAMTSLKLL
                     EWRVHGGQRDALVLMLLGYLLVMARMLYDQGPVTALWALASVALTLAAQVRVASDGGG
                     PPVAAVARLVGGLLARAVPLALVLFVLFPRVPGPLWSLPQDAAARTGVGEDMSPGTIA
                     ELSRSDAVAFRVRFTGGLPAADRRYWRGPVLDRYDGRTWRMSEELPGEHFPQTEASQA
                     LSYVVTLEPSGRRWLYALDLPDSLPAQTGVTASGMLIRRSAVTEVVRYEQRSVLEYRT
                     GPLASWDRSRLQRLPPSAAPRAQALGREWRTRFADPADRVQAALTMFRREPFRYTLSP
                     PLTPTDAVDEFLFDTRAGFCEHYAGAFVALMRAAEVPARVVTGYLGGEPSPGGDYFIV
                     RQSDAHAWAEVWLEGRGWTRIDPTAAIAPERVERGTASAIPDPDSLSLLSRRGESWVA
                     IALLKLDALEYAWNEWVLAYGPERQRELLGWLLGWRDIDWRGLILLLVSALAIVGLAL
                     ALLNRRSRRQGRDPLGRSYARFCARLAAIGLARQASEGPLSYRARIARLRPDLADATG
                     QILVRYAELRFGEHPDPQRLRELNAQLRRFRPRA"
     gene            complement(7668..8633)
                     /locus_tag="PK144_07995"
     CDS             complement(7668..8633)
                     /locus_tag="PK144_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF65705.1"
                     /translation="MSVEPRILRWHRRRHPLLRTAHPLTRRSIYILPTRTGLVFAAVL
                     LAMLGWSINYNASLGFALVFLLGTIALEAMRRTHDNLLGLTVHPPLAEPVFAGDTAFI
                     RLRLEAPAGEIRYGVVVRTAAGVETGGDLDGPLAALDLPVPAQRRGRLASGALRVSTC
                     FPLGLFTAWTWVEFQPSGLVWPQPAGVAQLPAGTHGKPQQQPARTESAGDEDFAGLRP
                     WRRGDPATHVAWKPSARSGQLQVKQFSAPPGGDELMLDLASAPGDGIEARLSQLTLWT
                     IEADRADVRFGLRLGATELAPARGSAHRFEVLERLALYGEGADAA"
     gene            complement(8637..9557)
                     /locus_tag="PK144_08000"
     CDS             complement(8637..9557)
                     /locus_tag="PK144_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF65706.1"
                     /translation="MRERLDAIIRQLGSIILGKEPALRLALACLLARGHLLIEDLPGM
                     GKTTLAQALARTLGLEYRRIQFTSDMLPADILGASVYDREQGAFVFHPGPVFAQLVLA
                     DEINRASPKTQSALLEAMEEGQVTSEGHTRRLPQPFFVIATQNPATQLGTFPLPESQL
                     DRFLLRVRLGYPDPKAERALLAGEDRRRLLDRLPVLIDPPGLLLLQARVERVHAADVL
                     LDYVQALISATRHGGQFETGLSPRAGLALLRVARAWALLAGRDHVLPEDVQVTLPALA
                     GHRLRPGAQNAGARADELLQRLVNSVPLPV"
     gene            complement(9665..10192)
                     /locus_tag="PK144_08005"
     CDS             complement(9665..10192)
                     /locus_tag="PK144_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNF65707.1"
                     /translation="MRYRLGDRRVVCPADGDWFIADDALVIGSVTLGVEASIWYRAVV
                     RGDSEQISIGARSNVQDGAVLHADPGFPLTIGADVTVGHLAMLHGCTIGDGTLVGIQS
                     TILNGAVIGEGCLIGAHTLIPERKVIPPRSLVMGVPGRVIRELDEAAVAGLLRSAAHY
                     VANFRRHRLDLQVQD"
     gene            complement(10200..10982)
                     /locus_tag="PK144_08010"
     CDS             complement(10200..10982)
                     /locus_tag="PK144_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF65708.1"
                     /translation="MTDPSATPVLPAAVVHGCAAGPAVPDVLLIHGAGGDHRHFPPAL
                     TDLPGVRVHALDLPGHGAAEGRFADSVEVYADAVAAYVESRGLTRVVLAGHSMGGAVV
                     MTLALRAPAWLRAQVLIGTGSRLKVLPLLFDLIDDDWPGAVATIVSGLFGPDADPAQV
                     EAVRGRYLDGPPARLRADLRACTRFDIGERLGTLRLPTLVVSGALDVMTPPKYGRALA
                     AAIPGAHHVEIAGAGHMLALERPQAVRAAIAGFLTTLDHAQE"
     gene            complement(11128..11203)
                     /locus_tag="PK144_08015"
     tRNA            complement(11128..11203)
                     /locus_tag="PK144_08015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11168..11170),aa:Gly,seq:gcc)
BASE COUNT         1685 a         3989 c         4116 g         1452 t
ORIGIN      
        1 aattccccgc atgcgcaggg atgaaccgaa gtcggcaagc atgaaaggct ggcgtgaaaa
       61 aaattcccgc atgcctcgac ctgaaccggc tcagccgccc ctcactcccg ccgctgccac
      121 ggggccgggc cgatttcgcc ggtatgtacc gggaattcgc cgcggctgcg gtcggcgaag
      181 taatggcgca gggtttcgcg cacggtctgg aaagccagtt cgtcccaggg aatccgggct
      241 tcgtcgtaca ggccgacttc gaggctttcc tcgccggcgg cgaattcggt gtcgagcagg
      301 cgggcgcggt acagcaggtg gacctggctg atgtgcggca ggctgtagag cgtgtacagg
      361 ccgaggattt cgacgcgggc gcaggcttcc tcgaaggttt cgcgggcggc agcggcgtcg
      421 gtggtttcgc cgttttccat gaagccggcg ggcagcgtcc agaagccgcg gcgcggttcg
      481 atcgcgcgcc ggcacagcaa cacccgcccc tgccactcgg ggatgcagcc ggcgacgatg
      541 cgcgggttct ggtagtggat cgtgccgcat tcgatgcaga cgtggcgcgg caggcggtcg
      601 ccctccggga tctgcacgat gaccggcgcg ctgcagctgc tgcagaactt catcgctcgc
      661 tcctcctgcg tttgtccaca cagcctgtgg acaaacacgt ggaaaacccg tgacggccac
      721 ccgccgaagc cgcgccgttg ctggcgaaat gacgatttgg cgacattttg cccacttgcg
      781 ttcttattga gaatcaatga cttgatataa gccattgatt ttcgacccgg cgacatcggc
      841 cgatcaggga cttgcagaac cgggcgatgc acttgtgcat aagtccgcgc ccggcggcgg
      901 gcgccatctt gcgcaagtgc ccgccgggct gcaatcgcag gcgcgcgcag gcgaccggaa
      961 gcgggtgctt gagccggctc cggcactcgt gcagaatccg cgcccccctg ccgcctgccc
     1021 tcccccgacg ccccagccat gcacgccaga gtcctgcgcg ccgacctgct gctgctgctg
     1081 accgcggcca tctggggctc cggcttcgtc gcccagcgcc tgggcatgga gagcatcggg
     1141 ccgatgctct acagcgcgct gcgcttcgcg ctcggcgggc tggtggtgct gccgctggcg
     1201 ctgcgcgccg atccgcggcc ctcggcgcgg ccgctggcgc cgcgcgccgc gctcggggcg
     1261 gccctgctcg ccgggctggt gctgttcggg ggcgtgacgc tgcagcagat cggcttgcag
     1321 tacacctcga tcgccaatgc cggcttcatc accggcctgt acgtggtgct ggtgccgctg
     1381 ctcggcctgc tgaccggcca gcgggcgccg gcagtggtgt ggatcggggc cggactcgcg
     1441 gtgatcgggt tgttcctgct cagcgtgcgc ggtgacttca cggtcgccgc cggcgactgg
     1501 ctgcagctcg ccggcgcgct gtgctgggcc gtgcacgtgc tgctgatcga tgcgctggcg
     1561 ccgcgcacgc acgcgctgcg cctcgcctgc cggcagttct tcgcctgcgc ggcggcgagc
     1621 ctgatcgcgg cgctgctgtt cgagccgatc gaatgggccg gcatcgtcgc tgcggcgccg
     1681 gccatcgcct ggggcgggct gctgtcggtc ggcaccggct acacgctgca ggtcatcgcg
     1741 cagcaggacg cgccggccgc gcacgcggcg ctgctgatga gcctcgaagc cgtgttcgcg
     1801 gcgctggccg gctggtggtg gctcggcgag ggactcgatg cgcgcgcgct cgccggctgc
     1861 acgctgatgc tcgccggcat gctgctcgcg cagggtctgg ccggcggcgg ccggccggcg
     1921 ggcggctgat accgatcaca cgtgaatgcg ttgttgatcc ccctctcccc aacccctccc
     1981 ccgcgagtgg ggaggggctg aaaagatcgg atgttcagat gtgaggggta tgaggcggct
     2041 gccgcgtcgc agccgcgccg gcctcagcgg ccgccgtgcg acacggcgta atgcggcagc
     2101 agttcgcggg cggcggcgag cacggcatcg agctgggccg gatcgacggc ttcgagatcg
     2161 aacagcgcca tcatgtccgg caggcgccgg tcgacggtct ccagcacgcc ggagagcacc
     2221 tcggtgtcga gcccgagcca gacatcgccc gcgagccagg cgcgttcggt ggcggcctcg
     2281 tgatcggcat ccagccacag atcggcgatg acgcccgagg cggcgacgca gtgcaccagc
     2341 gtgcgccgcg ccggcgccac gccgcgcggg tcgtgcgagc gcgccacggc ctcgatcagg
     2401 agttccggca acccccactg gccgagcagc caggcgccga cctcggcgtg gtcggtgccg
     2461 agcgccgcac gttcggcggc ggcgcgggcg gcatggccgt cgcagccggc gacgatgcgc
     2521 gcgtagtcgg cgggcagcgc ctgttcgagc gcgaggatgc cgaggtcctg cagcagcgcg
     2581 gcgacgaaca cgtcctcgac tgcgagcggc gcgaggcgat gcgcgagttc gcgcccgacc
     2641 agcgccgcat acaccgagcg tcgccagaaa tccggactgg tgccggccgg ctgcatcagg
     2701 ccgcgcagcg agaagccgag cgcgacgtgc gtgatgccct tggtgcccat gaccaccagc
     2761 gcctggcgca ggttggtgat gcggcgcgga tggttgtaga gcaccgagtt tgcagcgcgc
     2821 agcacgcgcg ccgtcatcgg cgggtcgtgc tggatctcgt cgagcagcgg ctcggcctcg
     2881 acatcgggat cgtgggcaag ggcgacgacg cgcatcgcca cgttcggcag gctggcgagc
     2941 gcaccggcct gacgcagacg ggtttcgagg gttctgtcca tcggatcgcg gggcctggtt
     3001 ggtccgctgg cagggccggc cgctggggac ggccgggacc ggtcgccgcg cagagccggg
     3061 gctcagggcg accagaaata gcgtacgacg tgaaagaaca ccggcgcgga aaagcacagt
     3121 gaatcgatgc gatcgagcac gccgccgtgg ccctcgatca tcgccccgaa gtccttgatg
     3181 ccggcatcgc gcttgatcgc cgacatcacc agcccgccgg cgaagccgag caggcagacg
     3241 agcagcgcga tgccgaaggc ctgcccgtgc gtgaacggcg tcaccggtgc gagcagcatg
     3301 ccgatcaggc tcgccgagcc gatgccgccg gcgaagccct cgacggtctt gttcggcgaa
     3361 agccgcggca cgatcggccg ccggccggcg agcttgcccc agacgtactg cagcacgtcc
     3421 gacagctgca cgaccagcag cagccagagc agcagcgcga cccggctgcc gtcgaagccc
     3481 ggcagctcga gcgtcagcag cgccggagcg tggctgacgc agtacacgca gaccatcagc
     3541 gcccactgcg tgcgcgccgt gcgcgccaga aaaccctggg catcggcatt gagcgcgctg
     3601 cgcagcggca ggaacaggaa gccgtagacc gggatcagga tcgtgaacat gccgtaccag
     3661 ccggtgccga ccagccagta ctgcagcggc aggaatacga agaagaccca gaacagcggc
     3721 tcgtgatcgg cgcggcgcgt cggcgtcagc gtcacgaact cgcgcagcgc cagaaacgag
     3781 atggccccga acagcagcac cgcaccgctc aggccgccgg cgagcgcccc tgcgaccagt
     3841 gcgcacatca gccaccaggc gcggatgcgg gcgttgaggt tctcgatggt cgcgcgtccg
     3901 gcgccatccc gcggccggcg cgccagcccg cggccgatca ggctcgcgat cacgagcagc
     3961 acgaacacgc cgccgagcag cgcgcgcacc ggggcgtcgt accaggtcag cggattcatg
     4021 ggttcgggtt caccgggcca gcgccagcac ggcctggcgc gcgcgttcga ggtaatcggc
     4081 ccgtgtctcg ccatcgaccg gcaggaagcg cgcgccgaag cgcgccgcgc acagcagcgg
     4141 caccggcagc atcgagccct tcggcagcac gcggttgagg ttttcgaggt ggaccgggat
     4201 cagctcggca cgcgggcagc gctgcgcgag ctgccagagt ccggagcgga agggctgcac
     4261 ggtctcgccg tcgccgcgcg tgccctcggg gaacaggatc agcgcctcgc cgcgttcgag
     4321 caccgcgagc agcggtgcca gcacgtcggc ccgggcagca tcggcttcgc cggcagccgt
     4381 ggccggctgg cggtcgatca gcaccgcgcc gaacacctgc agcgccagcc agcggcgcag
     4441 cggcgtcgca tcccagtaat cgcgcgcggc gaccggatgc gtgcgggtgc gcagcgccgg
     4501 cggcagcgcc gcccagatca ccaggccgtc gaggtggctc gcgtgattgg cgaagtacac
     4561 gcgcgcgccc gcagccggca ggctgccgag ccacagcgca cgcacgccgg tcaggccgcg
     4621 gcagagtccg accaggagca gcgccgcacc acggcgtgcg agcctgcgca tcagcgggcc
     4681 cggatgcggt ccagcgtgcc gctgaagctg cagtcggcgt cgcgcagcgt cgcgcagccc
     4741 tcgccctgtt cgctgatgct ggcctggccg gcctgcacgc cgatgacgat gcggcaggct
     4801 tccttgccgg tcgcaggctc gacggtgatc gtgcggccgt cgcgcagacc gtgcgcgacg
     4861 aactcgccgc ggcagcggcc gccttcggtg cgcgggatgt cgaggctcat cgtcacgctg
     4921 acctggccgt cgtcgtgctc gctcaggtcg agatggcgtt cgccgtcggc gcgtccgcgg
     4981 tattcgccgt cgagggcgcc ggcaccggcg aggggcgtga cggcgacgag cagcagggca
     5041 ggcagcatgc gcttcatggg attcatgaga ttcaaatcct gatccggatc ctgtttcaga
     5101 gccctgcatc ggtgcggttc gcgacgaagg cgaccagctc cggaaagaag actgcgaaat
     5161 ccccggccca gtccggcagc agcgccgtca ccgccggccc ggccgccgcc agcggcgacg
     5221 ggcggctcag gcgcgtggcg atgccggcca gggcccgggc gacgccggcg ggttcgcggt
     5281 acgaaagcag tgtccggccc tgcagcagat ggtcgatgct gcgctgcatg cggggcggca
     5341 gacgatcata atccgcgcgc aaggccgcgt gtaccgcccc gacgaaggcc ggcagcgact
     5401 gtgcggacca gttcgcccag tcccgggcca ggcagtggtc gtaggccacg tcgatgatga
     5461 tgccgccgta gcggcgcagc ggcgccggca gccgggcccg gctcgcgcat acgagcggat
     5521 gcgcatcggt ccagacatcg atcgcccggt gttcgcgcac cgccgcccgc cagcgcgcat
     5581 cggcgagggt ttcgaccggc ccgcggtgga agtcgccgag catcgcgccg acccgtgcgt
     5641 gcgggtcggg accggccaga tagaaatgtg cgagccagtt cacgggccgc ccgcacgggc
     5701 ttcaggcgcg cggccggaag cggcgcagct gcgcgttcag ctcgcgcagg cgctgcgggt
     5761 ccggatgctc gccgaagcgc agttcggcgt agcgcacgag gatctgtccg gtggcatcgg
     5821 cgagatccgg gcgcagccgg gcgatgcgcg cgcgatagga cagcggcccc tcgctggcct
     5881 gccgcgccag gccgatcgcg gccaggcgcg cgcagaagcg cgcatagctg cgcccgagcg
     5941 gatcgcggcc ctgccggcgg ctgcgccggt tcagcagggc gagcgcgagt ccgacgatgg
     6001 cgagcgccga gaccagcagc aggatcaggc cgcgccagtc gatgtcgcgc cagccgagca
     6061 gccagccgag cagctcgcgc tgacgttcgg gaccgtaggc gagcacccat tcgttccacg
     6121 catattcgag cgcatcgagc ttgagcagcg cgatcgcgac ccagctctcg ccgcggcgcg
     6181 acagcagcga caggctgtcg gggtccggga tcgccgaggc cgtgccgcgc tcgacgcgct
     6241 cgggcgcgat cgccgcggtc gggtcgatgc gcgtccagcc gcggccctcc agccagacct
     6301 cggcccaggc gtgggcgtcg gactggcgga cgatgaagta atcgccgccc ggcgagggct
     6361 cgccgccgag atagccggtg accacgcgcg ccggcacctc ggcggcacgc atcagcgcca
     6421 cgaaggcgcc ggcgtagtgt tcgcagaagc cggcgcgggt atcgaacagg aattcatcga
     6481 ctgcatcggt cggcgtcagc ggcggcgaca gcgtgtagcg gaagggctcg cgccggaaca
     6541 tcgtcagcgc cgcctgcacg cgatcggccg gatcggcgaa gcgcgtgcgc cattcgcgcc
     6601 cgagcgcctg cgcgcgcggt gcggcgctcg ggggcaggcg ctgcagacgg ctgcgatccc
     6661 agctcgccag cggcccggtg cggtattcga gcaccgagcg ctgctcgtag cgcacgacct
     6721 cggtcacggc gctgcgccgg atcagcatgc cggaggcggt gacgccggtc tgcgccggca
     6781 gcgagtccgg caggtcgagc gcgtacagcc agcgccggcc gctcggttcg agcgtgacca
     6841 cgtagctcag cgcctggctg gcttcggtct gcggaaagtg ctcgcccggc agttcctcgc
     6901 tcatgcgcca ggtgcggccg tcgtagcgat cgagcaccgg cccgcgccag tagcgacgat
     6961 ccgcagccgg caggccgccg gtgaagcgca cgcggaaggc gacggcatcg gagcggctca
     7021 gctcggcgat cgtgcccggt gacatgtcct cgccgacgcc ggtgcgggcg gcagcatcct
     7081 gcggcaggct ccacagcggc cccggcacgc gcgggaacag cacgaacagc accagcgcca
     7141 gcggtacggc gcgcgcgagc agaccgccga ccagccgcgc gacggccgcc accggcggtc
     7201 cgccgccgtc gctcgcgacg cgcacctgcg cagccagcgt cagcgcgacg ctcgccagcg
     7261 cccacagcgc cgtcaccggg ccctggtcat agagcatgcg cgccatgacc agcagatagc
     7321 cgagcagcat cagcaccagc gcatcgcgct gcccgccgtg cacgcgccat tcgagcagct
     7381 tcagcgaggt catcgcgtac agcagcgcga cgccggcctc gcgcccgaac agcgtgcggt
     7441 actgccagcc gatcgcgccg cacagcgcga cggtcgccgc cgccagcagc cagcggttcg
     7501 gcagcgccca gtcgcgtgcc tcggcgccga gccgccagag gccggtggcg atgaacaccg
     7561 ccgccagcca cagcggcacg accgtcacat gcggcgccat cgcgcagagc agcgcgagcc
     7621 ccggcaggcc gtagagccgg gcaccgggtg ccgggcgggt ccaggcttca ggccgcatcg
     7681 gcgccctccc cgtacagcgc caggcgctcc agcacctcga agcggtgcgc gctgccgcgc
     7741 gccggcgcga gttccgtggc gccgaggcgc aggccgaagc gcacatcggc ccggtcggcc
     7801 tcgatcgtcc acagcgtcag ctgcgacagc cgcgcctcga tgccgtcgcc gggcgcgctc
     7861 gccagatcga gcatcagctc gtcgccgccg ggcggcgcgc tgaactgctt gacctgcagc
     7921 tggccgctgc gcgccgacgg cttccacgcg acgtgcgtgg ccggatcgcc gcgccgccag
     7981 ggccgcaggc cggcgaagtc ctcgtcaccg gcgctctcgg tgcgcgccgg ctgctgctgc
     8041 ggcttgccgt gcgtgccggc cggcagctgc gccacgccgg ccggctgcgg ccagaccagc
     8101 ccggaaggct ggaactcgac ccaggtccag gccgtgaaca gcccgagcgg aaagcaggtc
     8161 gacacgcgca gcgccccgct cgcgagccgc ccgcggcgct gcgccggcac cggcaggtcg
     8221 agcgcggcca gcggtccgtc gagatcgccg ccggtctcga cgccggcagc ggtgcgcacg
     8281 accacgccgt agcggatctc gccggccggg gcctccagac gcaggcggat gaaagcggtg
     8341 tcgccggcga acaccggctc ggccagcggc ggatgcacgg tcagcccgag caggttgtcg
     8401 tgcgtgcggc gcatcgcctc aagcgcgatc gtgccgagca ggaacaccag cgcgaagccg
     8461 aggctggcgt tgtagttgat cgaccagccg agcatcgcca gcagcaccgc ggcgaacacg
     8521 aggccggtgc gcgtcggcag gatgtagatc gaacgccgcg tcagcggatg cgcggtgcgc
     8581 agcagcggat gccggcgacg gtgccagcgc aggatgcgcg gttcgacgct catcgatcag
     8641 accggcagcg gcaccgagtt gaccaggcgc tgcagcagct cgtcggcccg cgcgccggcg
     8701 ttctgcgcgc cggggcgcag gcgatgcccg gcgagcgccg gcagcgtcac ctgcacgtcc
     8761 tcgggcagca cgtgatcccg cccggcgagc agcgcccagg cacgcgcgac ccgcagcagc
     8821 gccagcccgg cgcgcggcga caggccggtc tcgaactggc cgccgtggcg ggtcgccgag
     8881 atcagcgcct gcacgtaatc gagcagcacg tcggcggcat gcacgcgctc gacgcgcgcc
     8941 tgcagcagca gcagcccggg cggatcgatc agcaccggca gccggtcgag caggcggcgg
     9001 cggtcctcgc cggcgagcag cgcgcgctcg gccttcggat ccgggtagcc gaggcgcacg
     9061 cgcagcagga agcgatcgag ctgcgattcc ggcagcggga aggtgccgag ctgggtcgcc
     9121 gggttctgcg tcgcgatcac gaagaacggc tgcggcagcc gccgcgtgtg accctcgctg
     9181 gtgacctgac cttcctccat cgcttcgagc agcgcgctct gcgtcttcgg cgaggcgcgg
     9241 ttgatctcgt cggcgagcac gagctgcgcg aacaccggcc ccggatggaa cacgaaggcg
     9301 ccctgctcgc ggtcgtagac cgaagcgccg aggatgtcgg ccggcagcat gtcgctggtg
     9361 aactggatgc gccggtattc gagcccgagc gtgcgcgcca gcgcctgcgc gagcgtggtc
     9421 ttgcccatgc cgggcaggtc ctcgatcagc aggtggccgc gcgcgagcag gcaggcgagc
     9481 gccaggcgca gcgccggctc cttgccgagg atgatgctgc cgagctggcg gatgatggca
     9541 tcgagccgct cgcgcagcgc ggccgtactg atcgcagttg ccggcgcggc agcggttccc
     9601 tgcacggctt ccaaggttct ttccatggtt ctcctgatcg cgtgcgcccg tggggcgggc
     9661 gctttcagtc ctggacctgc aggtccaggc ggtggcggcg gaaattggcg acgtaatgcg
     9721 cggcggagcg cagcaatccg gccacggcgg cctcgtccag ttcccggatc acgcgccccg
     9781 gcacgcccat gaccagcgag cgcggcggga tcaccttgcg ctcggggatc agcgtgtgcg
     9841 cgccgatcag gcagccctcg ccgatcacgg cgccgttgag gatcgtgctc tggatgccga
     9901 ccagcgtgcc gtcgccgatc gtgcagccgt gcagcatcgc cagatgcccg accgtcacgt
     9961 cagccccgat cgtcagcgga aagcccggat cggcatgcag cacggcgcca tcctgcacgt
    10021 tcgagcgcgc gccgatgctg atctgctcgc tgtcgccgcg cacgacggcg cgataccaga
    10081 tgctcgcctc gacgccgagc gtcaccgagc cgatgaccag tgcgtcatcg gcgatgaacc
    10141 agtcgccgtc cgcgggacag acgacgcgac gatccccgag ccggtagcgc atggcggcat
    10201 cactcctgag cgtgatcgag cgtggtgagg aatccggcga tggcggcgcg caccgcctgc
    10261 ggccgttcga gcgcgagcat gtgcccggcc ccggcgatct cgacgtggtg cgcgcccggg
    10321 atcgctgcgg cgagcgcgcg gccgtacttc ggcggcgtca tgacatcgag cgcgcccgag
    10381 accaccagcg tcggcaggcg cagcgtgccg agccgctcgc cgatatcgaa gcgggtgcag
    10441 gcccgcaggt cggcgcgcag ccgcgccggc gggccgtcga gatagcgccc gcgcacggcc
    10501 tcgacctgcg ccggatcggc atccggtccg aacaggccgc tgacgatggt cgcgaccgcg
    10561 cccggccagt cgtcgtcgat caggtcgaac agcagcggca gcaccttcag ccgcgaaccg
    10621 gtgccgatca gcacctgcgc ccgcagccag gccggcgcgc gcagcgccag cgtcatcacc
    10681 accgccccgc ccatcgagtg ccccgcgagc acgacgcgcg tgagcccgcg ggattcgacg
    10741 taggcggcga cggcatcggc gtagacctcg acgctgtcgg caaagcgccc ctcggccgcg
    10801 ccgtgaccgg gcaggtcgag tgcatgcacc cggacaccgg gcagatcggt cagggccggc
    10861 gggaagtggc gatgatcacc gccggcgccg tggatcagca gcacgtcggg gaccgctgga
    10921 ccggcggcgc aaccgtggac gacggcggcc ggcagcaccg gtgtggcgga cggatcggtc
    10981 atgagcgcgc tccgtggtac cggaacgccg gatgctagca gaaagcacag cccggcccgc
    11041 ctgcgccggg gccgtgatcg cgctcgcgat cgttaccggt gcgcaacaaa aaaccccggt
    11101 cgcgggaccg gggtttttcg tgaaatctgg agcgggaaac gaggctcgaa ctcgcgacct
    11161 caaccttggc aaggttgcgc tctaccaact gagctattcc cgcatgaatc gttgtggagc
    11221 gtcccttccg gggtcgtcca gg
//
LOCUS       DAOQIE010000101        11197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2887_length_11197_cov_7.289984, whole genome shotgun sequence.
ACCESSION   DAOQIE010000101 DAOQIE010000000
VERSION     DAOQIE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_11197_cov_7.289984"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1193
                     /gene="rsxD"
                     /locus_tag="PK144_08020"
     CDS             159..1193
                     /gene="rsxD"
                     /locus_tag="PK144_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HNF65709.1"
                     /translation="MLFETRTSPHVHLGNSVALVMRRVLYALAPGLLVLIVVFGPGVL
                     VQLALAAATALACETLMLLLRRRPLAPFLGDASALVTAALLAVALPPLGPWWLIVLGT
                     AFAIVIAKHLYGGLGYNPFNPAMIGYVVLLISFPLEMSTWAAPRGVGGHVAGFGEALA
                     LIFGGHGDHVLLDAVSSATPLDTLHTQLGLGQTVAAVRAGSPVFGWIGGSGWEWANLA
                     FLGGGLWMCQRRVAAWQIPAGMLGALALISTVFWIAEPARFAPPWFHVMSGAAVFGAF
                     FIATDPVSASTTPRGRLIYGAGIGLLTWLIRSLGGFPDGVAFAVLLMNLAAPTIDQYT
                     LPRAFGAGRA"
     gene            1199..1840
                     /gene="rsxG"
                     /locus_tag="PK144_08025"
     CDS             1199..1840
                     /gene="rsxG"
                     /locus_tag="PK144_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNF65710.1"
                     /translation="MSEVLQQMRRGGGVLGGFALLSVALVAATYFGTRGRIAASEQAA
                     LEAQLNALVPAARYDNHPTEDTIRLSDAAAFGSGEAVTVYRARRAGEPVAALATVIAP
                     DGYAGPIRLLVGVWADGRIAGVRVLAHKETPGLGDRIEIQRSDWIRSFDERSLTDPDA
                     AGWKVRKDGGRFDQFTGATITPRAIVKAVHGFLEYAQAHRAELYAPASGDRVP"
     gene            1837..2544
                     /locus_tag="PK144_08030"
     CDS             1837..2544
                     /locus_tag="PK144_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNF65711.1"
                     /translation="MSASAPSKSEILANGLWRNNTGLVALLGLCPLLATSSSAVNGLG
                     LGLATVLVLLLTNTTIALIRSFVPNEVRIPVFIVVIASAVTAVELAMHAFLPELHKVL
                     GIFIPLIVTNCCVLGRAESFASKNAPALAALDGVATGLGFTLVLTALGSLRELVGQGT
                     LFASADIMFGEAGRMLTVQVLHDYRGFLLAILPPGAFIGLALLIAGKNWLDARTNART
                     AATPVEAGCTPEAHARA"
     gene            2619..3257
                     /gene="nth"
                     /locus_tag="PK144_08035"
     CDS             2619..3257
                     /gene="nth"
                     /locus_tag="PK144_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNF65712.1"
                     /translation="MTPPQRREFFARLQAANPHPTTELQYASPFELLVAVILSAQATD
                     KSVNKATARLFPVANTPAQMLALGEDALRAHVQTIGLYRNKAANILKTCRLLLERHDG
                     EVPRTRAALEALPGVGRKTANVILNTAFGEPVIAVDTHIFRVANRTGLAPGRDVLAVE
                     RELERVVPDEYRQDAHHWLILHGRYVCTARRPQCPACVVRDLCACPDKTGDA"
     gene            3258..3728
                     /locus_tag="PK144_08040"
     CDS             3258..3728
                     /locus_tag="PK144_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNF65713.1"
                     /translation="MFGTDRNQLRRYYCETWRRHRAGETLEPLETLIATVIEAHPEYQ
                     ALLADPERALADEWTPQRGESNPFLHMGMHLGIQEQVQTDRPAGIRAAWLALARKLGD
                     PHAAEHAMMEALGEALWEAQRFGTAPDEAKYLARVQALAGIGPGPFPGSAGPRA"
     gene            3725..4282
                     /locus_tag="PK144_08045"
     CDS             3725..4282
                     /locus_tag="PK144_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF65714.1"
                     /translation="MSVPEVLEDLLARTALADRHAFEKLYRLSSAQLFGIVLRIVRDR
                     DLAADVLQETYVKIWHRAGDFRADLAQPLTWMGSIARHQAIDTLRRSARRLSTAEDID
                     EHYELADEAAESPADAVDRGLQGAALHRCLGELQGLQQRAVQLAWFRGLTHEDIAARL
                     DTPLGTVKSWLRRGLLRLKTCLEGA"
     gene            4279..5004
                     /locus_tag="PK144_08050"
     CDS             4279..5004
                     /locus_tag="PK144_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65715.1"
                     /translation="MNYRDPELRELLSAQYVLGTLKPRARRRFESLLPGRADLRRSVA
                     GWERRLPELLGPTPAIEAPDSVWPKLRTRLFPETLAAAPATTASPAPGLLARLSFWRG
                     WAAMASVVAFVFALALGLGKQTAIPGYASVVTSMQSHEPMWMLSAAPEMDRIYVKSIQ
                     PMVMSPDIGCLLWIRPEGSERMILVGELPWRGEEATLKIDKATRAQLKGQLVVTVERV
                     DRGMPDKPTAAPKFAGSWMPIRI"
     gene            complement(5060..6121)
                     /gene="mtnA"
                     /locus_tag="PK144_08055"
     CDS             complement(5060..6121)
                     /gene="mtnA"
                     /locus_tag="PK144_08055"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNF65716.1"
                     /translation="MSVLSDPAAHDRVRAVAWRDGALELLDQRLLPAREQRLRFDDAA
                     AVARAIRDMVVRGAPAIGIAAAYGVVLAARARFAADGEAWTQTIAADLERLADARPTA
                     VNLFWAIARMRRRIDALPAGVDPLPALLAEADAILAEDIAANRRMGELGAALIEPGRA
                     LLTHCNTGSLATGGYGTALGVIRSAWAGGRLTAVCADETRPWLQGARLTAWELVQDGI
                     PVTLIAEGAAAHRMKQGDIGWVVVGADRITADGSAANKIGTYGLAVLARFHGVRFMVV
                     APSSTIDMSLASGGDIPIEMRAPAELLELGGTRVAAEGAQAWNPSFDVTPAALIDAIV
                     TERGVVLAPDAAKMAALMA"
     gene            complement(6257..6955)
                     /locus_tag="PK144_08060"
     CDS             complement(6257..6955)
                     /locus_tag="PK144_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF65717.1"
                     /translation="MRVALLEDDRPQAEMMRTWLVEAGHDVQVFVQGKALVRELGRDS
                     YDLVVLDWMLPDIDGIQVLKWIREHVDWPMPVLFVTGKDEEDDIVQALDNGADDYMVK
                     PVKRMEMLARLKAVSRRAIAQDEGQKILEFGVYTVDLTTRAVLLNGQPIELTQKEFDL
                     TVFLFRGAGRVLSRGHILESVWGRSPDLNTRTVDTHVSRIRNKLDLRPEHGWQLKAVY
                     QHGYRLEPVEVPAT"
     gene            7179..7724
                     /locus_tag="PK144_08065"
     CDS             7179..7724
                     /locus_tag="PK144_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004140593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF65718.1"
                     /translation="MKLKLNNLALAAAVAIAVAAPAFADPEPAVTDPFGKAVKDPFDK
                     CVLAVGGTVLPECGPMVQKKRSELKLSGDTNFDFNKDTLKPAGKAELDKLVADMKQAK
                     QVDAVIVVGHTDSVGSDAYNQRLSERRAATVKNYLAAKGVPAGIIQASGRGESQPVAD
                     NATAAGRAQNRRVEITVEVTQ"
     gene            7873..9198
                     /locus_tag="PK144_08070"
     CDS             7873..9198
                     /locus_tag="PK144_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNF65719.1"
                     /translation="MQSIDTLLFPRWIVPVEPEAVVLDDMALAIDQGRILDLLPAAAA
                     RNRYHARNTLLLPNHCLLPGLVNAHTHLAMNLLRGYADDLPLMQWLTGHIWPAEARWV
                     GETFVHDGTLLAAAELIRGGVTCVNDMYFFPDVTARALGTAGLRAVVGLIAIDFPSAW
                     ASGPDEYLAKGLALHESLRHDPLLSTAFAPHAPYTVGEATLARIRALADELERPVHMH
                     VHETAGEIREAVAAHGCRPLARLDRLGLLSPALAAVHMTQLEDAEIARLAETGASVIH
                     CPESNLKLASGFCPVAKLEAAGVNVALGTDGAASNNDLDLFGEMRTAALLAKGVSGDA
                     AALPAHRALRMATLNGARALGLGEQTGSLVPGKWADVIAVDLGDLESRPLYHPISQLV
                     YASGRHQVTDVWVAGRQLLGNRELLTLDRSALLERAGHWRAQIGAGASA"
     gene            9234..9941
                     /gene="ubiG"
                     /locus_tag="PK144_08075"
     CDS             9234..9941
                     /gene="ubiG"
                     /locus_tag="PK144_08075"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014700795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNF65720.1"
                     /translation="MTTANVDQAEVAKFDALASRWWDPDGEFKPLHDINPLRLGFIKS
                     RAPLTDCAVLDVGCGGGVLSEALADEYAQVTGIDMAAAPLEIASLHLIESGLDVDYVQ
                     STAEDYADAHPDAFDVVTCMEMLEHVPEPASTIAACARLVRPGGHVFFSTLNRTPKAW
                     LLAVLGAEYLLRMLPRGTHEYAKFIRPSELARWAQLAGLEVREFTGVHYNPLSRRYTL
                     GPGLDVNYLVHCVRPQD"
     gene            10003..10674
                     /locus_tag="PK144_08080"
     CDS             10003..10674
                     /locus_tag="PK144_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNF65721.1"
                     /translation="MTAILFDLDGTLLDTAPDLVGALNRMRIEDGLPALDYASLRPAA
                     SHGTDALLAAGYGSTRHDAGHAALRERYLAQYRARIAERTAPFPGMDDTLATLEARGI
                     AWGIVTNKPSALTGALLAALGLDARACAVVSGDTLAVQKPDPAPLLHACTLAGLDPAR
                     CWYIGDAARDIEAGSRAGMRTLVAGWGYLGPDDEPAAWGADGTLAVPTELLDWLDHGP
                     EAAHD"
     gene            10667..>11197
                     /locus_tag="PK144_08085"
     CDS             10667..>11197
                     /locus_tag="PK144_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65722.1"
                     /translation="MTETPDDAGLAGRLAGLLPGALGGWLPAPVLADWSALATLSRTL
                     AGLEDSEARAEAIAALPPALARLAAADWWRPLPCGPVEDVATLLAEAQRAWRPYALAA
                     LAASGLDADERLRGQAVALGGGFFLLAQLGELTADCRRGTPWLALDELAKYRSSVAAL
                     AGGLTHPGFVAANLDRC"
BASE COUNT         1587 a         4220 c         3805 g         1585 t
ORIGIN      
        1 accggcagcc gccgaggcag cgccggcccc gggcgcaccg gcgctgagcg aggcgcagct
       61 cgcgaagctc gccgccgccc gcgcccgcgc cgaagcgaag aaggccgcgc aggccgtctg
      121 cgccccgtcc gatccgcaat cccgctgagt cgcaggccat gttgttcgaa acccgcacct
      181 cgccgcacgt ccacctcggc aacagcgtcg cgctggtcat gcgccgcgtg ctgtatgcgc
      241 tcgcgccggg cctgctggtg ctgatcgtcg tgttcggccc cggcgtactc gtgcagctcg
      301 cgctggccgc ggctacggcc ctggcctgcg agacgctgat gctgctgctg cgccgccggc
      361 cgctggcccc cttcctcggc gacgccagcg cgctggtcac ggccgcgctg ctggccgtgg
      421 cgctgccgcc gctcgggccg tggtggctga tcgtgctcgg cacggcgttc gcgatcgtca
      481 tcgccaagca cctgtacggc gggctcggct acaacccgtt caatccggcg atgatcggct
      541 acgtcgtgct gctgatctcc ttcccgctgg agatgagcac ctgggcagcc ccgcgcgggg
      601 tcggcggaca cgtcgccggc ttcggcgagg ccctcgccct gatcttcggt ggccacggcg
      661 atcacgtgct gctcgacgcc gtgagttcgg ctaccccgct cgacacgctg cacacgcaac
      721 tcggcctcgg ccagaccgtc gccgccgtgc gcgccggcag tccggtgttc ggctggatcg
      781 gcggcagcgg ctgggaatgg gcgaacctcg ccttcctcgg cggcgggctg tggatgtgcc
      841 agcggcgcgt cgccgcctgg cagatcccgg ccggcatgct cggcgcgctg gcgctgatct
      901 cgaccgtgtt ctggatcgcg gagcccgcgc gcttcgcacc gccgtggttc cacgtgatga
      961 gcggcgcggc ggtgttcggt gccttcttca tcgccaccga tccggtgtcg gccagcacga
     1021 cgccgcgcgg ccggctgatc tacggcgccg gcatcggcct gctgacctgg ctgatccgct
     1081 cgctcggcgg cttcccggat ggcgtggcgt ttgccgtgct gctgatgaac ctcgccgcac
     1141 cgaccatcga ccagtacacg ctgccgcgtg ccttcggcgc cgggagggcc tgaaagtcat
     1201 gagcgaggtc ctgcagcaga tgcgccgcgg gggcggcgtg ctcggcggtt tcgcgctgct
     1261 cagcgtcgcg ctggtcgccg ccacctactt cggcacgcgc gggcgcatcg ccgcctccga
     1321 acaggccgca ctcgaagcgc agctgaacgc gctggtgccc gccgcgcgct atgacaacca
     1381 tcccactgaa gacacgatcc ggctcagcga cgccgccgcc ttcggcagcg gcgaagcggt
     1441 gaccgtctac cgggcgcggc gcgccggcga gccggtcgcg gcgcttgcga ccgtcatcgc
     1501 gcccgacggc tacgccgggc cgatccggct gctggtcggc gtctgggccg atggccgcat
     1561 cgccggcgtg cgcgtgctcg cgcacaagga aacgccgggg ctcggcgatc gcatcgagat
     1621 ccagcgcagc gactggatcc gcagcttcga cgagcgcagc ctcaccgatc ccgacgccgc
     1681 cggctggaag gtgcgcaagg acggcgggcg cttcgaccag ttcaccggcg cgaccatcac
     1741 gccgcgcgcg atcgtgaagg ccgtgcacgg cttcctcgaa tacgcgcagg cgcatcgcgc
     1801 cgaactctac gccccggcca gcggagaccg agtcccatga gcgccagtgc cccgagcaag
     1861 tccgagatcc tcgccaacgg cctctggcgc aacaacaccg gtctggtcgc gctgctcggg
     1921 ctgtgtccgc tgctcgcgac ctccagctcg gcggtcaacg gcctcggcct ggggctggcg
     1981 acggtgctgg tgctgctgct gaccaacacg acgatcgcgc tgatccgcag cttcgtgccg
     2041 aacgaggtgc gcatcccggt gttcatcgtc gtgatcgcct cggcggtcac cgccgtcgaa
     2101 ctggcgatgc acgccttcct gcccgaactg cacaaggtgc tcggcatctt catcccgctg
     2161 atcgtcacca actgctgcgt gctcgggcgc gccgaatcct tcgcctccaa gaacgccccg
     2221 gcgctcgccg cgctcgacgg cgtcgcgacc gggctcggct tcacgctggt gctgaccgcg
     2281 ctcggcagcc tgcgcgaact cgtcggccag ggcacgctgt tcgcgagcgc cgacatcatg
     2341 ttcggcgagg ccggccgcat gctgaccgtg caggtgctgc acgactaccg cggcttcctg
     2401 ctcgcgatcc tgccgcccgg cgcgttcatc gggctggcgc tgctgatcgc cggcaagaac
     2461 tggctcgacg cccgtaccaa cgcccgcacg gccgcgaccc cggtcgaagc cggctgcacg
     2521 cccgaggccc acgcccgcgc ctgaacgccc ggggcgcatg atgggccggc ggccggcttg
     2581 cgctacgata agcgcctttc cgcgccgccc gcccgccgat gaccccgccg caacgccgcg
     2641 aattcttcgc ccgcctgcag gctgcgaacc cgcatccgac caccgaactg cagtacgcga
     2701 gcccgttcga gctgctggtg gcggtgatcc tgtcggcgca ggccaccgac aagagcgtca
     2761 acaaggccac ggcccggctg ttcccggtcg cgaacacgcc ggcgcagatg ctcgcgctcg
     2821 gcgaggacgc cctgcgcgcc cacgtgcaga ccatcggcct gtaccgcaac aaggccgcga
     2881 acatcctgaa gacctgccgg ctgctgctgg agcgccacga cggcgaggtg ccgcgcacgc
     2941 gcgccgcgct cgaagccctg cccggcgtcg ggcgcaagac cgcgaacgtc atcctcaaca
     3001 ccgccttcgg cgagccggtg atcgcggtcg acacgcacat cttccgcgtc gccaaccgca
     3061 ccggcctcgc ccccgggcgc gacgtgctcg ccgtcgagcg cgagctcgag cgcgtcgtgc
     3121 ccgatgaata ccgtcaggac gcgcaccact ggctgatcct gcacggccgc tacgtctgca
     3181 ccgcgcgccg gccgcaatgc ccggcctgcg tggtgcgcga cctctgcgcc tgccccgaca
     3241 agaccggaga cgcctgaatg ttcggcaccg accgcaacca gctgcgccgc tactactgcg
     3301 agacctggcg ccggcaccgc gccggcgaga ccctcgaacc gctcgaaacg ctgatcgcca
     3361 ccgtcatcga ggcgcatccg gaataccagg ccctgctcgc cgatcccgag cgcgcactcg
     3421 ccgacgaatg gacgccgcag cgcggcgaga gcaatccctt cctgcacatg ggcatgcacc
     3481 tcggcatcca ggagcaggtg cagaccgacc gcccggccgg catccgcgcc gcctggctcg
     3541 cgctcgcccg caagctcggc gacccgcacg ccgccgagca cgcgatgatg gaagccctcg
     3601 gcgaggcgct gtgggaggcg cagcgcttcg gcacggcgcc ggacgaggcg aagtatctcg
     3661 cccgcgtgca ggcgctcgcc ggcatcgggc cgggaccgtt cccgggctcg gcggggccgc
     3721 gtgcatgagc gtgcccgagg tgctcgaaga cctgctggcg cgcacggcgc tcgcggatcg
     3781 ccatgcattc gaaaagctgt atcgactgtc gtcggcgcaa ctgttcggca tcgtgctgcg
     3841 tatcgtccgt gaccgcgacc tcgccgccga cgtgctgcag gagacctacg tgaagatctg
     3901 gcaccgcgcc ggcgatttcc gcgccgatct cgcgcagccg ctgacctgga tgggctcgat
     3961 cgcccgccat caggcgatcg acacgctgcg gcgcagcgcc cggcgcctga gcacggccga
     4021 ggacatcgac gagcactacg agcttgccga cgaggcggcc gagagtccgg ccgacgccgt
     4081 cgatcgcggc ctgcagggtg cggccctgca ccgctgcctc ggcgagctgc agggcctgca
     4141 gcagcgcgcc gtgcagctcg cgtggtttcg cggcctcacg cacgaggaca tcgccgcgcg
     4201 gctggacacg ccgctcggta ccgtcaagtc ctggctgcgc cgcggcctgc tgcggctcaa
     4261 gacctgtctg gagggcgcat gaactaccgc gaccccgaac tccgtgaact gctgtcggcg
     4321 cagtacgtgc tcggcacgct gaaaccgcgc gcgcgccggc gcttcgagag cctgctgccc
     4381 ggccgcgccg acctgcgccg cagcgtcgcc ggctgggaac gccggctgcc ggaactgctc
     4441 ggcccgacgc cggcgatcga ggcaccggac agcgtctggc cgaagctgcg cacgcgcctg
     4501 ttcccggaaa cgctcgcggc ggccccggcg acgaccgctt cgcctgcgcc cggcctgctc
     4561 gcgcgcctga gcttctggcg cggctgggcg gcgatggcca gtgtcgtcgc cttcgtgttc
     4621 gcgctggcgc tcggcctcgg caagcagacc gcgatccccg gctacgccag cgtcgtcacc
     4681 agcatgcagt cccacgaacc gatgtggatg ctgtcggcgg ccccggagat ggatcgcatc
     4741 tacgtcaaga gcatccagcc gatggtcatg tcgccggaca tcggctgcct gctgtggatc
     4801 cggcccgagg gctcggagcg catgatcctc gtcggcgaac tgccgtggcg cggcgaggag
     4861 gcgacgctga agatcgacaa ggcgacgcgc gcgcagctca agggccagct cgtcgtcacc
     4921 gtcgagcgcg tcgatcgcgg catgcccgac aagccgaccg cggcaccgaa gttcgccggc
     4981 agctggatgc cgatccgcat ctgaaggcac ccgggcaggg cgcggaagac aggcccgctg
     5041 caggcgcccc cgccgccctt caggccatca gcgccgccat cttcgcggca tccggcgcga
     5101 gcaccacgcc gcgctcggtg acgatcgcgt cgatcagcgc cgccggcgtc acgtcgaagg
     5161 acggattcca ggcctgcgcg ccttcggccg cgacgcgcgt gccgccgagt tcgagcagct
     5221 ccgcgggggc acgcatctcg atcgggatgt ccccgccgct cgcgaggctc atgtcgatcg
     5281 tcgagctcgg cgccacgacc atgaacctca cgccgtggaa gcgcgccagc acggcgagcc
     5341 cgtaggtgcc gatcttgttg gcggccgagc cgtcggcggt gatgcggtcg gcaccgacca
     5401 cgacccagcc gatgtcgccc tgcttcatgc ggtgcgcggc cgcgccctcg gcgatcagcg
     5461 tcaccgggat gccgtcctgc accagctccc aggccgtcag ccgcgcgccc tgcagccacg
     5521 gccgcgtctc gtcggcgcag acggccgtga gccgcccgcc ggcccaggcg ctgcggatca
     5581 cgccgagcgc ggtgccgtag ccgccggtgg cgagcgagcc ggtgttgcag tgcgtgagca
     5641 gcgcccggcc cggctcgatc agcgccgcgc ccagctcgcc catgcgccgg ttggcggcga
     5701 tgtcctcggc gaggatggcg tcggcctcgg cgagcagcgc cggcagcgga tcgacgccgg
     5761 ccggcagcgc gtcgatgcgc ctgcgcatgc gcgcgatcgc ccagaacagg ttgaccgcgg
     5821 tcgggcgcgc atcggcgagc cgttcgagat cggccgcaat cgtctgcgtc cacgcctcgc
     5881 catcggccgc aaagcgcgcg cgggccgcga gcacgacgcc gtaggccgcg gcgatgccga
     5941 tcgccggcgc accgcgcacg accatgtcgc ggatcgcgcg cgcgacggct gcggcatcgt
     6001 cgaagcgcag ccgctgctcc cgggccggca gcaggcgctg gtcgagcagt tcgagcgcac
     6061 cgtcgcgcca ggcgacggca cggacgcggt catgcgcggc agggtcggac aggacggaca
     6121 tcggcaggac tccccggact accggaaggg ccgcggcagg cggccggttc gatggagggg
     6181 cgccagtgta gcgatccgtc gggcacgaaa aacccccgcg gcccgcatcg ggcagcgggg
     6241 gtcctgcggc cggggctcag gtggccggga cttcgaccgg ttccagccgg tagccgtgct
     6301 ggtagacggc cttcagctgc cagccgtgct cggggcgcag gtcgagcttg ttgcggatgc
     6361 ggctgacgtg cgtgtcgacc gtgcgcgtgt tgaggtcggg gctgcggccc cagacgctct
     6421 ccaggatgtg gccgcgcgac aacacgcgcc cggcgccccg gaacaggaac acggtcaggt
     6481 cgaattcctt ctgcgtcagc tcgatcggct ggccgttcag cagcacggcg cgcgtggtca
     6541 ggtcgaccgt gtagacgccg aactcgagga tcttctgccc ctcgtcctgc gcgatcgccc
     6601 ggcgcgacac cgccttcagc cgcgccagca tctccatgcg cttgaccggc ttgaccatgt
     6661 agtcgtcggc gccgttgtcg agcgcctgca cgatgtcgtc ctcctcgtcc ttgccggtca
     6721 cgaacagcac cggcatcggc cagtcgacgt gctcgcggat ccacttgagc acctgaatgc
     6781 cgtcgatgtc cggcagcatc cagtcgagca cgaccagatc gtaggaatca cggccgagtt
     6841 cgcggaccag ggccttgccc tggacgaaca cctgtacgtc gtggccggcc tcgaccagcc
     6901 acgtccgcat catctccgcc tgggggcggt catcctcaag cagtgcgacg cgcaaggcac
     6961 tctcccttta ttccaatcgc aacacttttt gcaattctga cacaggggtg tatcatccga
     7021 aactaggaaa accgattttt ccgttatact cggcccgact tgggagccgg tagcaaagga
     7081 tgccgaccgc cccttccgaa accatcccga gaagaagtgt caacgccggc tggttagacc
     7141 gttccggact ttgcttgcag tgacttggag gttagacgat gaaacttaag cttaacaacc
     7201 ttgccctggc cgcggccgtt gccatcgcag ttgcggcccc ggccttcgcc gaccccgagc
     7261 cggccgtcac cgacccgttc ggcaaggcgg tcaaggaccc gttcgacaag tgcgtgctcg
     7321 ccgtcggcgg caccgtgctg ccggaatgcg gcccgatggt gcagaagaag cgctcggaac
     7381 tgaagctttc gggtgacacg aacttcgact tcaacaagga cacgctgaag cccgccggca
     7441 aggccgagct cgacaagctc gtcgccgaca tgaagcaggc caagcaggtc gatgccgtca
     7501 tcgtcgtcgg tcacaccgac agcgtcggct ccgatgccta caaccagcgc ctgtccgagc
     7561 gtcgcgccgc gaccgtcaag aactacctgg ccgcgaaggg cgtcccggcc ggcatcatcc
     7621 aggcctcggg ccgcggcgag tcgcagccgg ttgccgacaa cgcgacggcc gccggtcgcg
     7681 cgcagaaccg ccgcgtcgag atcaccgtcg aagtcacgca gtaagcttcg acctcgcctc
     7741 gcacaccccg cacgccccgg ccatcggccg gggcgtcgtc atttggccgg ccgccggcgg
     7801 atgcagaacc gctgccgcag gcacttcgca gaaccggcat aatcgctgcc atgcgccgag
     7861 ccggagccct ccatgcagtc catcgacacc ctgttgtttc cgcgctggat cgtgccggtc
     7921 gagcccgaag cggtcgtgct cgacgacatg gcgctcgcga tcgaccaggg ccgcatcctc
     7981 gacctgctgc cggccgctgc cgcgcgcaac cggtatcacg cgcgcaacac gctgctgctg
     8041 ccgaatcact gcctgctgcc gggcctcgtc aacgcgcaca cgcatctggc gatgaacctg
     8101 ctgcgcggct acgccgatga cctgccgctg atgcagtggc tgaccggcca catctggccg
     8161 gccgaggcgc gctgggtcgg cgagaccttc gtgcatgacg gcacgctgct cgccgcggcc
     8221 gagctgatcc gcggcggcgt cacctgcgtc aacgacatgt atttcttccc ggacgtgacg
     8281 gcgcgcgcgc tcggcacggc cggcctgcgc gccgtggtcg gcctgatcgc catcgacttc
     8341 ccgagcgcct gggcctcggg gccggacgaa tacctcgcca agggcctggc gctgcacgaa
     8401 tccctgcgcc acgacccgct gctgagcacc gcgttcgcgc cgcacgcgcc gtacacggtc
     8461 ggcgaggcca cgctcgcacg catccgcgcg ctggccgacg agctcgaacg cccggtgcac
     8521 atgcacgtgc acgagaccgc cggcgagatc cgggaggccg tcgccgcgca cggctgccgg
     8581 ccgctggcgc gcctcgaccg cctcggactg ctgtcgccgg ccttggccgc cgtgcacatg
     8641 acccagctcg aagacgccga gatcgcccgc ctcgccgaga ccggcgccag cgtcatccac
     8701 tgccccgaat cgaacctgaa gctcgcctcc ggcttctgcc cggtcgcgaa gctcgaagcc
     8761 gccggcgtca acgtcgcgct cggcaccgac ggcgcggcca gcaacaacga tctcgacctg
     8821 ttcggcgaga tgcgcaccgc cgccctgctc gccaagggcg tcagcggcga tgccgcggcg
     8881 ctgccggcgc accgggcgct gcgcatggcg acgctcaacg gcgcgcgcgc gctcgggctc
     8941 ggcgagcaga ccggctcgct ggtgccgggc aagtgggccg atgtcatcgc cgtcgatctc
     9001 ggcgatctcg aaagccggcc gctgtatcac ccgatctcgc agctcgtcta cgcgagcggc
     9061 cgccaccagg tcaccgatgt ctgggtcgcc ggccgccagc tgctcggcaa ccgcgagctg
     9121 ctgacgctgg atcgcagcgc gctgctcgaa cgcgccgggc actggcgcgc gcagatcggt
     9181 gccggcgcga gcgcctgaac ccgcaagttt ccgccttgac cgaggaacgc cgcatgacga
     9241 ccgccaacgt cgatcaggcc gaagtcgcca agttcgatgc cctcgccagc cgctggtggg
     9301 atccggacgg cgagttcaag ccgctgcacg acatcaaccc gctgcgcctc ggcttcatca
     9361 agtcgcgcgc cccgctgacg gactgcgcgg tgctcgatgt cggctgcggc ggcggcgtgc
     9421 tgagcgaagc cctcgccgat gaatacgcgc aggtcaccgg catcgacatg gccgctgcac
     9481 cgctcgaaat cgccagcctg cacctgatcg aatccgggct cgatgtcgat tacgtgcagt
     9541 cgacggccga ggactacgcc gacgcgcatc cggacgcctt cgacgtggtc acctgcatgg
     9601 agatgctcga acacgtgccg gaaccggcct cgaccatcgc cgcctgcgcg cggctggtgc
     9661 gcccgggcgg gcacgtgttc ttctcgacgc tgaaccgcac gccgaaggcc tggctgctgg
     9721 ccgtgctcgg ggccgaatac ctgctgcgca tgctgccgcg cggcacgcac gagtacgcga
     9781 agttcatccg cccctcggag ctggcgcgct gggcgcagct cgccgggctg gaggtgcgcg
     9841 agttcaccgg cgtgcactac aacccgctgt cgcgccgcta cacgctcggc cccgggctcg
     9901 acgtgaacta cctcgtgcac tgcgtgcggc cgcaggactg ataccggtca cgaccgaacg
     9961 cccgagcctt tcagcccctc ccctgacgac ggaagccccc gcttgaccgc catcctgttc
    10021 gatctcgacg gcacgctgct cgacaccgcg ccggatctgg tcggcgcgct gaaccgcatg
    10081 cgcatcgagg acggcctgcc ggccctcgac tacgccagcc tgcgcccggc cgcctcgcac
    10141 ggcaccgacg cgctgctcgc ggccggctac ggcagcacgc gccatgacgc cggccacgcc
    10201 gcgctgcgcg agcgctatct cgcgcagtac cgcgcacgca tcgccgagcg caccgcgccg
    10261 tttcccggca tggacgacac gctggccacg ctggaggcac gcggcatcgc ctggggcatc
    10321 gtcaccaaca agccctcggc gctgaccggc gcgctgctcg cggcgctcgg gctggacgcc
    10381 cgcgcctgcg cggtggtcag cggcgacacg ctggcggtgc agaagcccga tccggcgccg
    10441 ctgctgcacg cctgcacgct cgccggtctc gatccggcgc gctgctggta catcggcgac
    10501 gccgcgcgcg acatcgaggc cggcagccgc gccggcatgc gcacgctggt ggccggctgg
    10561 ggctacctcg gcccggacga cgaacccgcg gcctggggcg ccgacggcac gctcgccgtg
    10621 ccgaccgaac tgctcgactg gctggaccac ggtccggagg ccgctcatga ctgaaacgcc
    10681 ggacgatgcc ggacttgccg ggcgcctcgc cggcctgctg cccggagcgc tcggcggctg
    10741 gctgccggcg ccggtgctcg cggactggtc ggcgctggcc acgctcagcc gcacgctggc
    10801 aggcctggag gattccgagg cccgcgccga ggcgatcgcg gcactgccgc cggcgctggc
    10861 ccgcctcgcc gccgccgact ggtggcgacc gctgccgtgc ggtccggtcg aggacgtggc
    10921 gacgctgctc gccgaggcgc agcgcgcgtg gcgcccgtat gcgctcgccg cactcgcggc
    10981 gagcgggctc gatgccgacg agcgcctgcg cggtcaggca gtcgcgctcg gcggcggctt
    11041 cttcctgctc gcgcagctcg gcgagctgac cgcggactgc cggcgcggca cgccgtggct
    11101 cgcgctcgat gagcttgcga agtaccgcag cagcgtcgcg gcgctggccg gcggcctcac
    11161 gcacccgggc ttcgtcgccg ccaacctcga tcgctgc
//
LOCUS       DAOQIE010000102        11149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2905_length_11149_cov_7.422481, whole genome shotgun sequence.
ACCESSION   DAOQIE010000102 DAOQIE010000000
VERSION     DAOQIE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_11149_cov_7.422481"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK144_08090"
     CDS             complement(<1..450)
                     /locus_tag="PK144_08090"
                     /inference="COORDINATES: protein motif:HMM:NF040448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HNF65723.1"
                     /translation="MSTDPFLRRLLRTVADDGSAGFGRRAQPGEWAVPGACAFAAEDP
                     AQLPAARAAAFESGFLGLGSQGWSAEVEVVDIDARTLDGLTLRLAAWLREEVGAPDLD
                     TALAVAREELDYALQLAADAAPGSRLRVTRRLRDGDIHEEYRLLGSAT"
     gene            complement(447..1352)
                     /locus_tag="PK144_08095"
     CDS             complement(447..1352)
                     /locus_tag="PK144_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNF65724.1"
                     /translation="MATPPARRPLLSHAGRPAQYPITVVDESGTARDIHIPMERALTL
                     YVDRRELVTLMTLGLEPEWLALGYLRTQGLVDALDDIAAVQVDWEVEAVAVRTRQGIE
                     DWDARLGVRTVTTGCGQGTMFGRLSERAVSVRIEAPVLRQSLLYALGERLRLRDTLYK
                     HTGSIHGCALCAGDEVLCFVEDVGRHNAVDAIAGWMWLEGVEGAGKLFYTTGRLTSEM
                     VLKTAQMGVPVLVSRSGTTQAAVELARRIGVTLIGRLRNRHFFVYHGAERVRFDAVPP
                     ALPRTQGGKEHLPADEAALSPEGRP"
     gene            1568..2110
                     /locus_tag="PK144_08100"
     CDS             1568..2110
                     /locus_tag="PK144_08100"
                     /inference="COORDINATES: protein motif:HMM:NF023177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HNF65725.1"
                     /translation="MTASDPGSLPEGAGTAADALPARVPVAVLMERVPVSGNRWIDHR
                     WQCAAVLVGDPASASAAPAIIDDGHGRELRIWRGLTLQLWPDEAESYRHNLDGTQPGL
                     FVATRPGADGAPEPFLVSACGDHASALEEMSEAVHRVALPPGLMPWLEAWIAAHWKPE
                     PRRKRQRTDWKEGGGPGPQS"
     gene            complement(2118..3047)
                     /locus_tag="PK144_08105"
     CDS             complement(2118..3047)
                     /locus_tag="PK144_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF65726.1"
                     /translation="MDLIDDLNLFVRVVEAGGMSAAARALGLSTQIVSQRVQRLEARI
                     GVRLLHRTTRQLQPTEAGERFLRQARALVEDAGALLAGFSAERDRITGTLRMTLPASF
                     GQRHVAPLLPEFLALHPQLALSLRFSDRLVDLVAGGLDLGIRIGTLTDSTLASRRLAA
                     NRRLLCASPAYLARAGQPQNPEALSAHECLVLVDGSERQDDWRLFGPGGRECRVRVRG
                     RLEADHGGALREAALGGLGIALKSTWDVAADLAAGRLLPVLPDWQPEPTAIWAVFPQR
                     RHLPPRVSACIEFFAARFGPLPPWERMLATQAG"
     gene            3231..4244
                     /locus_tag="PK144_08110"
     CDS             3231..4244
                     /locus_tag="PK144_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HNF65727.1"
                     /translation="MRAVGLTRYLPVDDPQSLIDCTLPRPGPGPHDLLVRVAAVSVNP
                     VDTKVRAPKAAVEATPRVLGWDAAGTVEAIGSEVTLFRPGDEVWYAGSIVRPGSNAEF
                     QCVDERIVALKPRSLDFAQAAALPLTTITAWEALFERLGLDADGADAGRSLLIIGGAG
                     GVGSIAIQLARRAGLTTIVTASRPETIEWCRALGATHVIDHRQPLPAQLEALGFRQVD
                     AIANFANTDAYWAVMCELIAPQGRICAIVENRAPLELGLLKAKSAGFVWEFMFTRAQF
                     GTPDMIAQHRLLTRVAGLVDAGELRTTLRETLGRIDAANLREAHRRLERGTGIGKLVL
                     AGW"
     gene            4362..5393
                     /locus_tag="PK144_08115"
     CDS             4362..5393
                     /locus_tag="PK144_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF65728.1"
                     /translation="MSRRFPLLCAALFAALGLHALPSAAADAPVLNVYSARHYQTDEA
                     LYAGFTEQTGIRVNRIEGKEDELLERLKNEGAASPADVLVTVDAARLAKADALGLFAP
                     VESKTLAARIPEYLRTPTWIAFSTRARVIVYAKDSVNPADVASYESLADPKLKGKVCS
                     RPGAHPYNLSLMAGLIGHLGEAKATEWAQGLVANFARPPRGGDTDQIKAVAAGECGVA
                     ISNSYYLARLMRSDKPEDQAVVAKVGMVWPNQASYGTHINVSGAGVLKHAPNREAAVK
                     FLEYLASDRAQVYFADGNNEWPAVAGVKVDNPALAKLGPFKSDTQSVGVLAANQAEAL
                     KIFDRVGFR"
     gene            complement(5348..5608)
                     /locus_tag="PK144_08120"
     CDS             complement(5348..5608)
                     /locus_tag="PK144_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65729.1"
                     /translation="MLPLLIPPAALLWWSFGSSPLLNEPAMLRLRDHAVLAFPLTQLL
                     YLSLLALAPLIEHRLRRQAMSVSAVPEVQRNPTRSKIFSASA"
     gene            6064..6840
                     /locus_tag="PK144_08125"
     CDS             6064..6840
                     /locus_tag="PK144_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65730.1"
                     /translation="MSAEHVLETRGLVKEFRGFVAVDHVDLQVRRGHIHALIGPNGAG
                     KTTVFNLLTKFLTPTRGTILFNGTDITAERPAQIARRGIVRSFQISAVFPHLTALENV
                     RVALQRKLGTSFHFWKSPKTLDRLDARADELLDAVGLAQYRDTVTVEMPYGRKRALEI
                     ATTLALEPELMLLDEPTQGMGHEDVDRVAELIRRVAEGRTILMVEHNLSVVSRLADRI
                     TVLARGATLAEGDYASVSQDPRVLEAYIGKAVDDTAGAHA"
     gene            6837..7559
                     /locus_tag="PK144_08130"
     CDS             6837..7559
                     /locus_tag="PK144_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65731.1"
                     /translation="MTSAFQPDFEQLRVENLHAYYGESHILHGIALDVRRGELVTLLG
                     RNGAGKTTTLRAILGLTGRRSGAIHLNGQNIIDWPTHRIAHLGVGYCPEERGIFASLS
                     VEENLVLPPAVRSGGMSVEELYAMFPNLAERRKSQGTRLSGGEQQMLAMARILRTGAN
                     LLLLDEITEGLAPVIVQRLGEVLTLLKSRGYTVILVEQNFRFAAPLADRHYVIEHGRV
                     VDTIGRAELPAKMEALHQRLGV"
     gene            7648..8862
                     /locus_tag="PK144_08135"
     CDS             7648..8862
                     /locus_tag="PK144_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF65732.1"
                     /translation="MRHFRTVLSTAVAAALGSALAQAAISDDVIKIGVLTDMSGTYSD
                     LAGPGVVAAAEMAIADAGGKVAGKPIVMLKADHQNKADIAANKAREWNDVEKVDAYVD
                     LVTTSTALAVMEIAKNANKVVLVSGAGSSRITNEDCNPVTVHWTYDTYAMAKGTGSAM
                     VKEGGKTWFFLTADYAFGQNLQKDVSAVVEASGGKVLGAVKHPFPASDFSSFILQAQA
                     SGAQVIGLANAGADTTNAIKAANEFGIVAGGQKLAGLLVFISDVHSLGLPVAQGLTLT
                     TGFYWDYDDQTRAWSKRFFEKTGKMPTMVQAGVYSSLMHYFKAIEAAGSDDGPTVIKQ
                     MKSGPVEDFFSRHGKIRDDGRLVHDMFLAEVKKPSESKAPWDYYKILRVIPGDEAYLP
                     LSESKCPLVRKS"
     gene            8931..9815
                     /locus_tag="PK144_08140"
     CDS             8931..9815
                     /locus_tag="PK144_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNF65733.1"
                     /translation="MDFLNIPLPAVLAQLVVGLINGAFYALLSLGLAIIFGLLNIINF
                     AHGAQYMLGAFFAWFGVQYLGIDYWFALLLVPIAVGAIGMLLERFLLSKLYREDHLYG
                     LLLTFGLALIIEGVFVQLYGVSGKPYAVPEALRGALNLGFMFLPKYRAWVIVASVVVC
                     LGAWLVIERTKLGAYLRAGTENPRLLQAFGVNVPMMITLTYGFGVALAAFAGVLAAPI
                     FQVSPKMGSDLIIVVFAVVVIGGMGSILGSILTGLGLGLVEGLTKVVWPEASSTVIFV
                     IMAIVLLVRPAGLFGRER"
     gene            9846..10790
                     /locus_tag="PK144_08145"
     CDS             9846..10790
                     /locus_tag="PK144_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNF65734.1"
                     /translation="MYGKYTHAIHAGLLLIALIAPFGIYPVFLMKMLCFALYACAFNL
                     LLGYAGLLSFGHAAFFGLAAYTTGHVLKELGLPTPLGLALGTLAAGALGFVVGLLAIR
                     RQGIYFAMITLALSQMVYFFFLQAPFTGGENGLQGIPRGSFFGLDLGNDLTLYYVVLG
                     IFVAAYALIQRTVHSPFGQVLKAIKENEPRAISLGYEVDRYKLLAMVLSASLAGLAGA
                     LKSVVLQLASLGDAHWHASGEVVLMTLLGGMGTLLGPVVGAGTVVSLQNYLASSPIGA
                     YLPAPVVLGAIFVICVLAFRRGFVGELLEVLRKSFNIR"
BASE COUNT         1695 a         3971 c         3745 g         1738 t
ORIGIN      
        1 cgtcgcgctg ccgagcagcc ggtattcctc gtggatgtcg ccgtcgcgga ggcgccgcgt
       61 cacgcgcagc cgcgagccgg gtgcggcgtc ggcggcgagc tgcagcgcgt agtcgagttc
      121 ctcgcgcgcg acggcgagcg cggtgtcgag atccggggcg ccgacctcct cgcgcagcca
      181 ggcggcgagg cgcagcgtca ggccgtcgag cgtgcgcgca tcgatgtcga cgacctcgac
      241 ctcggcgctc cagccctgcg agccgagacc gagaaagccg ctttcgaagg ccgcggcccg
      301 cgccgccggc agttgcgccg ggtcctcggc ggcgaatgca caggcgccgg gcacggccca
      361 ttcccccggc tgcgcgcgcc gtccgaaacc ggcactgccg tcgtcggcga cggtgcgcag
      421 cagccggcgc aggaagggat cagtgctcat gggcggccct ccggggacag tgcggcctca
      481 tcggccggca ggtgttcctt gccaccctgc gtgcgcggca gcgcgggcgg cacggcgtcg
      541 aaccgcacgc gttcggcgcc gtgatagacg aagaagtggc ggttgcgcag ccggccgatc
      601 agcgtcacgc cgatgcgccg cgccagttcg accgccgcct gcgtcgtgcc cgagcgcgac
      661 accagcaccg gcacgcccat ctgcgcggtc ttcagcacca tctccgaggt cagccgcccg
      721 gtggtgtaga acagcttgcc ggcgccctcg acgccttcga gccacatcca gccggcgatc
      781 gcgtcgacgg cgttgtggcg gccgacgtcc tcgacgaagc agagcacctc gtcaccggcg
      841 cagagcgcgc agccgtggat cgagccggtg tgcttgtaca gcgtgtcgcg cagccgcagc
      901 cgctcgccga gcgcatacag cagcgactgg cgcagcacgg gcgcctcgat gcggaccgaa
      961 acggcccgct cgctgaggcg gccgaacatc gtgccctggc cgcagccggt ggtgacggtg
     1021 cgcacgccga ggcgcgcatc ccagtcctcg atgccctggc gcgtgcgcac ggccacggcc
     1081 tcgacctccc agtcgacctg cacggcggcg atgtcatcga gcgcatcgac gaggccctgc
     1141 gtgcgcaggt agccgagcgc cagccactcc ggttcgaggc cgagcgtcat cagcgtcacc
     1201 agctcgcgcc ggtccacgta cagcgtcagc gcgcgttcca tcgggatgtg gatgtcccgc
     1261 gccgtgccgc tctcgtcgac gacggtgatc gggtattgcg cgggccggcc ggcgtggctc
     1321 agcagcgggc ggcgggcagg gggcgtggcc atgaaacgct tccgcaaggg ctgaaggcgg
     1381 acagagtgca tcttcccgcc ccggcagatg caaatctgcc gggtcaaagt gacgcgatcc
     1441 ggaaggccgc cggctgcggg cggccatgat tagcacgatc cctgcccgat ccggtccgca
     1501 ctgctgcgac tggcgccgca acggcgattg cgtatcgtgg gcgctcctgc caccgagggc
     1561 tgccgccatg actgcctccg atcccggttc cctgcccgaa ggcgcgggga ccgctgccga
     1621 tgccctgccg gcccgcgtgc cggtcgccgt gctgatggag cgcgtgccgg tcagcggcaa
     1681 ccgctggatc gatcatcgct ggcagtgcgc ggccgtgctg gtcggtgacc cggccagcgc
     1741 cagcgcggca ccggcgatca tcgacgatgg ccatggccgc gagctgcgca tctggcgggg
     1801 gctcacgctg cagctgtggc cggacgaggc cgaaagctac cgccacaacc tcgatggcac
     1861 gcagccgggc ctgttcgttg cgacccgccc cggcgcggac ggcgcgcccg agccctttct
     1921 ggtcagcgcc tgcggcgatc acgccagcgc gctcgaggag atgagcgagg ccgtgcatcg
     1981 cgtcgcgctg ccacccgggc tcatgccgtg gctggaggcg tggatcgccg cgcactggaa
     2041 gccggaaccg cgcaggaagc gccagcgcac ggactggaag gagggcggcg gtccgggccc
     2101 gcagtcctga gccttcctca gccggcctgc gttgccagca tccgctccca gggcggcagc
     2161 ggtccgaagc gcgccgcgaa gaactcgatg caggccgaca cccgcggcgg caggtgccgg
     2221 cgctgcggga acaccgccca gatcgcggtc ggctccggct gccagtccgg cagcaccggc
     2281 agcagccggc cggcggcgag gtctgcggcc acgtcccagg tcgatttcag cgcgatgccg
     2341 agaccgccga gtgcagcctc gcgcagcgcc ccgccgtggt cggcctccag ccggccgcgc
     2401 acgcgcacgc ggcactcgcg cccgccgggg ccgaacagcc gccagtcgtc ctgacgctcg
     2461 ctgccatcga ccagcaccag gcattcgtgc gcgctcaggg cctcgggatt ttgcggctgg
     2521 ccggcgcggg ccagataggc gggacttgcg cacagcagcc ggcggttcgc tgccagccgg
     2581 cggctcgcca gcgtcgaatc ggtcagcgtg ccgatgcgga tgccgagatc gaggccgccg
     2641 gcgacgagat cgacgagccg gtcgctgaag cgcagcgaca gcgcaagctg cgggtgcagc
     2701 gccaggaact ccggcagcag cggcgcgacg tggcgctggc cgaacgaggc cggcagcgtc
     2761 atgcgcagcg tgccggtgat gcggtcgcgc tcggcgctga agccggcgag cagtgccccg
     2821 gcgtcctcga ccagcgcgcg ggcctgacgc aggaagcgct cgccggcctc ggtcggctgc
     2881 agctgccggg tcgtgcggtg cagcaggcgc acgccgatcc gcgcttcgag ccgctgcacg
     2941 cgctggctga cgatctgcgt cgacagcccg agcgcccgtg ccgccgcact catgccgccg
     3001 gcctcgacga cgcgcacgaa caggttcaga tcgtcgatca ggtccagggg agtctcctgc
     3061 cgtgctgtgc cggcgcaagc cctggtgcag ggcgcgcatc atcaaggatt tcttgatagt
     3121 gaatcgccgg attgcgtctt tatcaagcga tcctgccgct tcaggctgtg ctcaccttca
     3181 acgaatgccg gagccccgcc atgaagccca gcgatcagga cagccgcacg atgcgcgccg
     3241 tcggcctgac ccgttatctg ccggttgacg atccgcagtc gctgatcgac tgcacgctgc
     3301 cgcggcccgg gcccggtccg cacgacctgc tggtgcgcgt cgccgccgtg tcggtgaatc
     3361 cggtcgacac caaggtgcgg gcgccgaagg ccgcggtcga agcgacgccg cgcgtgctcg
     3421 gctgggatgc cgccggcacc gtcgaggcga tcggcagcga agtgacgctg ttccggcccg
     3481 gggacgaggt ctggtatgcc ggcagcatcg tgcgccccgg cagcaacgcc gaattccagt
     3541 gcgtcgatga acgcatcgtc gcgctgaaac cgcgctcgct cgattttgcg caggccgcgg
     3601 cgctgccgct gacgacgatc accgcctggg aggccttgtt cgagcgtctc ggcctcgatg
     3661 cggacggtgc cgatgccggg cgcagcctgc tgatcatcgg cggggccggc ggcgtcggtt
     3721 cgatcgcgat ccagctcgcc cggcgcgccg gcctgacgac gatcgtgacc gcctcgcggc
     3781 cggaaaccat cgaatggtgt cgtgcgctcg gtgcgaccca cgtcatcgat caccgccagc
     3841 cgctgccggc gcagctcgaa gcgctcggtt tccggcaggt cgatgcgatc gcgaactttg
     3901 ccaataccga tgcctactgg gcggtgatgt gcgagctgat cgcgccgcag ggccgcatct
     3961 gcgcgatcgt cgagaaccgc gcgccgctcg aactcgggct gctgaaggcc aagagcgccg
     4021 gtttcgtctg ggaattcatg ttcacgcgcg cgcagttcgg cacgccggac atgatcgcgc
     4081 agcaccggct gctgacgcgc gtcgccgggc tcgtcgatgc cggggaactg cgcacgacgc
     4141 tgcgcgagac gctcgggcgc atcgatgccg ccaacctgcg cgaggcgcac cgccggctgg
     4201 agcgcggcac cggcatcggc aagctggtgc tggccggctg gtgagccatg cggctgtcat
     4261 cgccatgcgg atgacagccg ttctcattta cagcgggaga tccattcggc agactccgcc
     4321 cgaccctcga atggtcgatc acctgtaagg agtcctgtcc gatgtcgcgt cgatttccgc
     4381 tgctgtgcgc cgccctgttt gcggcgctcg gcctgcatgc cctgccctcg gccgcggccg
     4441 atgcgccggt gctgaacgtc tattccgccc ggcattacca gaccgacgag gcgctgtatg
     4501 ccggtttcac cgagcagacc ggcatccgcg tcaaccgcat cgaaggcaag gaggacgaac
     4561 tgctggagcg cctgaagaac gaaggcgccg ccagtccggc cgacgtgctg gtcaccgtcg
     4621 atgccgcgcg tctggccaag gccgatgcgc tcggactgtt cgcgccggtc gaatcgaaga
     4681 cgctggccgc gcgcatcccc gagtacctgc gcacgccgac ctggatcgcg ttctcgacgc
     4741 gcgcccgcgt catcgtctac gccaaggatt cggtgaatcc ggccgatgtc gccagctatg
     4801 aatcactcgc cgatccgaag ctcaagggca aggtctgctc gcgccccggc gcgcacccgt
     4861 acaacctgtc gctgatggcc ggactgatcg ggcatctcgg cgaggcaaaa gcgaccgagt
     4921 gggcgcaggg cctggtcgcg aacttcgcgc gtccgccgcg cggcggcgac accgaccaga
     4981 tcaaggcggt cgcggccggc gagtgcggcg tggcgatctc gaacagctat tacctcgcgc
     5041 ggctgatgcg ctcggacaag cccgaggatc aggccgtggt cgcgaaggtc ggcatggtct
     5101 ggccgaacca ggcgagctac ggcacgcaca tcaacgtctc gggcgccggc gtgctgaagc
     5161 acgcgccgaa tcgcgaggcg gccgtgaagt tcctcgaata cctcgcgagc gaccgggcgc
     5221 aggtctactt cgccgacggc aacaacgagt ggccggcggt tgccggcgtc aaggtcgaca
     5281 acccggcgct cgcgaagctc ggaccgttca agtccgacac gcagtcggtc ggcgtgctgg
     5341 ccgcgaatca ggccgaggcg ctgaagatct tcgaccgcgt cggattccgc tgaacctcgg
     5401 gcactgcgga tacggacatc gcctgccggc gcaggcggtg ttcgatcagc ggtgcgagtg
     5461 cgagcaggct cagatacagc agttgcgtca gcgggaatgc cagcacggca tggtctcgca
     5521 gcctcagcat cgccggctcg ttcagcagcg ggctgctgcc gaagctccac cacagcagcg
     5581 ccgccggcgg tatcagcagc ggcagcatca gcaggcagcg gatcagcagg gagctgaagg
     5641 tctttatgat caggtggttg ccgatcagga cacagcagag gagcaaaagc agcagcatgt
     5701 cggcgttcct tcgcaacggg gagggccggc gggcgctgcc ggcggggtgt atctaatcct
     5761 gttgcaccat ttcagttcaa gctccggcat ggctcttctg aacttttttt cacgattccg
     5821 ggcctcaagt ccgcccggat cgggccgggg ccgcgtccgg agccggcgcc ggaggctcga
     5881 cccgcgcaag ccgatcccgc gcgggactgc ctgaaaacgc tgcttgcacc cggcaggcgc
     5941 cgtctacgct tgttcaaaac cattcaacag aagccgtcgc acgctgcgct tcgcagcgcc
     6001 tgcgtccggg gtttcgctcg cttcacatgc ctgccgtccg ccgacgacgg ccacgagggg
     6061 aagatgagtg cagagcacgt actcgaaacg cgcgggctcg tgaaggagtt ccgcggcttc
     6121 gtcgccgtcg atcacgtcga cctgcaggtg cgccgcggcc acatccacgc gctgatcggc
     6181 ccgaacggcg ccggcaagac caccgtcttc aacctgctga ccaagttcct gacgccgacg
     6241 cgcggcacga tcctgttcaa cggcaccgac atcaccgccg agcggccggc gcagatcgca
     6301 cggcgcggca tcgtgcgctc gttccagatc tcggcggtgt tcccgcatct caccgcgctc
     6361 gagaacgtgc gcgtcgcgct gcagcgcaag ctcggcactt cctttcattt ctggaagtcg
     6421 ccgaagacgc tcgatcgcct cgatgcccgc gccgatgaac tgctcgatgc cgtggggctg
     6481 gcgcagtacc gcgacaccgt gacggtcgag atgccgtacg ggcgcaagcg cgcgctggag
     6541 atcgcgacca cgctcgcgct cgaacccgag ctgatgctgc tcgacgagcc gacccagggc
     6601 atgggccacg aggatgtcga tcgcgtcgcc gagctgatcc gccgcgtcgc cgaggggcgc
     6661 accatcctga tggtcgagca caacctgagc gtggtgtcgc ggcttgccga tcgcatcacc
     6721 gtgctcgcgc gcggcgccac gctcgccgag ggcgactacg ccagcgtctc gcaggacccg
     6781 cgcgtgctgg aggcctacat cggcaaggcc gtcgatgaca ccgcgggagc ccacgcatga
     6841 ccagcgcctt ccagcccgac ttcgagcagc tgcgcgtcga gaacctgcat gcctattacg
     6901 gcgaatcgca catcctgcac ggcatcgcgc tcgacgtgcg ccgcggcgaa ctggtgacgc
     6961 tgctcgggcg caacggcgcc ggcaagacca cgacgctgcg cgcgatcctc ggtctcaccg
     7021 gccggcgcag cggcgccatc cacctcaacg gccagaacat catcgactgg ccgacgcacc
     7081 gcatcgccca cctcggcgtc ggctactgcc cggaggagcg cggcatcttc gcgagcctct
     7141 cggtcgagga gaacctcgtg ctgccgccgg ccgtgcgctc gggcggcatg agcgtcgagg
     7201 agctctacgc gatgttcccg aacctcgccg agcggcgcaa aagccagggc acgcgcctct
     7261 ccggcggcga gcagcagatg ctggcgatgg cgcgcatcct gcgcaccggc gccaacctgc
     7321 tgctgctcga cgagatcacc gaaggcctcg ccccggtcat cgtccagcgc ctcggcgagg
     7381 tgctgacgct gctgaagtcg cgcggctaca cggtgatcct cgtcgagcag aacttccgct
     7441 tcgccgcgcc actcgccgac cggcattacg tgatcgagca cggccgcgtc gttgacacca
     7501 tcgggcgcgc cgagctgccc gcaaagatgg aagccctgca ccagcgcctc ggcgtctgaa
     7561 ccgctcgtgc cctgcgccgg aacgcgccat tccaacaaga accacccgca gccctttccg
     7621 tccggaaacc cagaggagaa ccccccgatg cgtcacttcc gcaccgtgct gtccaccgcc
     7681 gtcgcggctg ccctgggcag cgccctcgca caggccgcga tctccgatga cgtgatcaag
     7741 atcggcgtgc tcaccgacat gtccggcacc tactccgatc ttgccggtcc cggcgtcgtc
     7801 gccgccgccg agatggccat cgccgatgcc ggcggcaagg tcgccggcaa gccgatcgtg
     7861 atgctgaagg ctgatcacca gaacaaggcc gacatcgccg ccaacaaggc gcgcgagtgg
     7921 aacgatgtcg agaaggtcga tgcctacgtc gatctggtca ccaccagcac cgcgctcgcg
     7981 gtcatggaga tcgccaagaa cgcgaacaag gtcgtgctgg tctccggggc cggctcctcg
     8041 cgcatcacca acgaggactg caaccccgtc accgtgcact ggacctacga cacctacgcg
     8101 atggccaagg gcaccggctc ggcgatggtc aaggagggcg gcaagacctg gttcttcctg
     8161 accgccgact acgccttcgg ccagaacctg cagaaggacg tcagtgccgt ggtcgaggcg
     8221 agcggcggca aggtgctcgg cgcggtcaag catccgttcc cggcgagcga cttcagctcc
     8281 ttcatcctgc aggcgcaggc ctcgggcgcg caggtcatcg gccttgccaa cgccggtgcc
     8341 gacaccacca acgccatcaa ggccgccaac gagttcggca tcgtcgccgg cggccagaag
     8401 ctcgccggcc tgctggtgtt catctccgac gtgcacagcc tcggcctgcc ggtcgcgcag
     8461 ggcctgacgc tgaccaccgg cttctactgg gactacgacg accagacccg cgcctggtcg
     8521 aagcgcttct tcgagaagac cggcaagatg ccgaccatgg tgcaggccgg cgtgtactcg
     8581 tcgctgatgc attacttcaa ggcgatcgag gccgccggct ccgatgacgg cccgaccgtc
     8641 atcaagcaga tgaagtccgg cccggtcgag gacttcttct cgcgccacgg caagatccgc
     8701 gatgacggcc gcctcgtgca cgacatgttc ctcgccgagg tcaagaagcc gtccgagtcg
     8761 aaggcgccgt gggactacta caagatcctg cgcgtgatcc ccggcgacga ggcctacctg
     8821 ccgctttccg agagcaagtg cccgctggtc aggaagtcgt aagcccctcc gtgcgcgcac
     8881 cggccggcgg cgccggtgtg cgcgcctgaa ccccgcgccg gaggctcgtc atggacttcc
     8941 tgaacatccc gctgccggcc gtgctcgcgc agctcgtcgt cggcctgatc aacggcgcct
     9001 tctacgcgct gctgtcgctc ggcctcgcga tcatcttcgg gctgctgaac atcatcaact
     9061 tcgcgcacgg cgcgcagtac atgctcggcg ccttcttcgc ctggttcggc gtgcagtacc
     9121 tcggcatcga ctactggttc gcgctgctgc tcgtgccgat cgcggtcggc gccatcggca
     9181 tgctgctgga gcgcttcctg ctctcgaagc tctaccgcga ggatcacctg tacgggctgc
     9241 tgctgacctt cgggctggcg ctgatcatcg agggcgtgtt cgtgcagctc tacggcgtct
     9301 cgggcaagcc ctacgccgtg cccgaggcgc tgcgcggggc gctcaatctc ggcttcatgt
     9361 tcctgcccaa gtaccgcgcc tgggtcatcg tcgcctcggt cgtcgtctgc ctcggcgcct
     9421 ggctggtgat cgagcgcacc aagctcggtg cctacctgcg cgccggcacc gagaacccgc
     9481 gcctgctgca ggccttcggc gtcaacgtgc cgatgatgat cacgctcacc tacggcttcg
     9541 gcgtcgcgct cgcggccttc gccggcgtgc tcgccgcgcc gatcttccag gtcagcccga
     9601 agatgggctc ggacctgatc atcgtcgtgt tcgcggtggt cgtcatcggc ggcatgggct
     9661 cgatcctcgg ctcgatcctc accggcctcg gtctcggcct ggtcgagggc ctgaccaagg
     9721 tcgtctggcc cgaggcttcg agcacggtga tcttcgtgat catggccatc gtgctgctgg
     9781 tacgcccggc cggcctgttc ggccgggaac gctgaccgat ccggcccgcg aggagaatcc
     9841 gaacgatgta cggcaagtac acccatgcca tccacgccgg cctgctgctg atcgcgctca
     9901 tcgcgccgtt cggcatctac ccggtgttcc tgatgaagat gctctgcttc gcgctctatg
     9961 cctgcgcgtt caacctgctg ctcggctacg cggggctgct gtccttcggg catgccgcct
    10021 tcttcgggct ggctgcgtac accaccggtc acgtgctgaa ggaactcggc ctgccgacac
    10081 cgctcggcct cgcgctcggc acgctcgccg ccggtgcgct cggcttcgtg gtcggcctgc
    10141 tggcgatccg ccgccagggc atctacttcg cgatgatcac gctcgccctg tcgcagatgg
    10201 tgtacttctt cttcctgcag gcgccgttca ccggcggcga gaacggcctg cagggcatcc
    10261 cgcgcggcag cttcttcgga ctcgatctcg gcaacgacct gacgctgtac tacgtcgtgc
    10321 tcggcatctt cgtcgccgcc tacgcgctga tccagcgcac cgtgcactcg ccgttcgggc
    10381 aggtgctgaa ggcgatcaag gagaacgagc cgcgggcgat ctcgctcggc tacgaggtcg
    10441 atcgctacaa gctgctggcg atggtgctgt cggcgagcct cgcggggctt gccggggcgc
    10501 tcaagagcgt cgtgctgcag ctcgcctcgc tcggcgatgc gcactggcac gcctcgggcg
    10561 aggtcgtgct gatgacgctg ctcggcggca tgggcacgct gctcggcccg gtggtcggcg
    10621 ccggcaccgt cgtcagcctg cagaactacc tcgcctccag cccgatcggc gcctatctgc
    10681 cggcaccggt cgtgctcggc gcgatcttcg tcatctgcgt gctcgccttc cgccgcggct
    10741 tcgtcggcga actgctcgaa gtgctgcgca agtccttcaa catccgctga acccccgcat
    10801 cgcgacggca gaggtctgtc gtcgccccgc ctgcctttgc ctgcgccgcg tccgtgccgg
    10861 agtccgcaag tccggactcc ggccacggcc tttgcctgac tgccgatgcg gccgccggca
    10921 cgcaggcggc acgctgccat cgtgagcttt gtctgctgct gcgcgagaga agccggcagc
    10981 ggcagggcgc tgcgttttcc ctgcgcagac gcggacgttt ccggtcatac ggagcgtgtc
    11041 accagttaca tcgagcgcat ccccagtcgt accgagcaca tccccagtca tatggagcac
    11101 atccccagtc atatggagca catccccagt catatggagc acatcccca
//
LOCUS       DAOQIE010000103        11062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2934_length_11062_cov_6.227492, whole genome shotgun sequence.
ACCESSION   DAOQIE010000103 DAOQIE010000000
VERSION     DAOQIE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2934_length_11062_cov_6.227492"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK144_08150"
     CDS             <1..878
                     /locus_tag="PK144_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNF65735.1"
                     /translation="GPFTVESLSPHRILGVDEDDELIDSLREPDAEYGARQSFPQMIL
                     ENLKTAGVQQAHKEDRISFTALMAWPGDMVCAEGRYLEAEGVEKRAAIFIGPEFGTVQ
                     RADLVEAAREAADAGFDVLIACAFNYEAHTTDFARLGKLPVLKARMNADLHMAEDLKN
                     TGKGNLFVIFGEPDITLLPEGDMLRVKVNGVDVFKPQTGEVISDGADGIACWFIDTDY
                     NEESFFVRHAYFLGANDPYSALKTTLKAEIDADAWASLNSDTSRPFRKPKSGRIAVKV
                     INHLGDEVMKVFRVA"
     gene            901..1191
                     /locus_tag="PK144_08155"
     CDS             901..1191
                     /locus_tag="PK144_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF65736.1"
                     /translation="MNTADAIRLLAESKPMLAERFGVKDLALFGSTARGSAGAASDVD
                     VLVAFDGPATSARYFGVQFYLEDLMGCAVDLVTDKALRPELRPFVEREAIHV"
     gene            1184..1537
                     /locus_tag="PK144_08160"
     CDS             1184..1537
                     /locus_tag="PK144_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNF65737.1"
                     /translation="MSDTLQREWRFYIDDMIDASRKVLAYTAGLDQAGFVASGLHYDA
                     SVRNIEIIGEAATHVPEDVRNGHPLIPWRQIVATRNRLIHGYLGIDNDTLWSIIRDDM
                     PDLLEALVRLKESQG"
     gene            1540..3657
                     /locus_tag="PK144_08165"
     CDS             1540..3657
                     /locus_tag="PK144_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF65738.1"
                     /translation="MEFRIADTFTDSLGRLTGDEQKLVKTTAFDLQLNPASPGMNFHK
                     LDRARDQNFWSVRVGSDIRLIVHRSETSLLLCYVDHHDKAYEWAERRKLETHPKTGAA
                     QLIEIRETVQEIVVPVYVQSELPLPATPAKPAPAAKLLFLAVSEDELLSYGVPPEWLP
                     DVKQATEDSLLALADHLPAEAAEALLELATGGKPRLPTPSTVNPFDHPDAQRRFRVMS
                     NVEELQSALDYPWEKWTVFLHPEQRQWVERDFNGPARVAGSAGTGKTIVALHRAAYLA
                     RQHPDARVLLTTFSNTLANALQSKLNRLLTHEPRLAERIDVHSLEAIGLRLHKTQVGV
                     AKLASREEILQLVGDAATAVPGHRFGRHFLLTEWEQVVDAWQLDHWEAYRDVVRLGRK
                     TRLPEAQRAVLWAIFEHVRAGLRDKGLVTASGLFTALAHALKARGNPVFDFAVVDEAQ
                     DIGVAHLRFLAALGGQFGGGEGAVRPNALFFAGDLGQRIFQQPFSWKSLGVDVRGRSR
                     TLRVNYRTSHQIRSQADRLLGPGVTDVDGNTEDRSDTVSVFNGPPPRIQIFNDAEKES
                     AAIGRWLAECGSAGILPHEIGVFVRSAAQIDRAEDAVRLAALPYQILDEHVATEHDHV
                     SISTMHLVKGLEFRAVAVMACDDEVIPLQERIETVGDNGDLQEVYDSERQLLYVACTR
                     ARDRLLVTGTKPSSEFLDDLTGG"
     gene            complement(4116..5768)
                     /locus_tag="PK144_08170"
     CDS             complement(4116..5768)
                     /locus_tag="PK144_08170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF65739.1"
                     /translation="MSLRNRILLATTVVLGSLLAALYGLGREELQRGYREAETAQART
                     HLESVRQLIDYELRALDDVTIDWSAWDETWRFALAPAQEYLDVNLTDGGLDAIRVDLF
                     ALYADSARPVFAVQRPLRTRPPGSEGGPAPSQDEAGEHDGLRIPPQLRRLPPEAVQAL
                     AQLPELPALARGESEVLRASVRLGAHAYLIAVRPVLTSQRQGPAHGALVLGRRLDATL
                     LASLSQVTHLPLRFEEPALDWPQADADIALARGEDGALVGRLRLHDPFGNTVAVLRSD
                     RLPDLGAQAAAAEKYLSAALLVLGGGCLLLVLLLLENWVLRRIAAIDAIVDAVEQSRD
                     TRQYRIHDAGQDELGRLGRAINRMLDTLHAAHLEIAHEAAHDPLTGLANRRRALDTLA
                     HVIATGGGPLALLIIDLDGFKQVNDTLGHIAGDMVLRTVATRLSAETRRTDLAARLGG
                     DEFLVLILGDTAEEDALWLARKVIAAIARPVPHAGTQARVSASIGIALYPQHGENSEA
                     LFAAADAAMYAAKRAGRDGFRVAGEVAAADNDADGGGEAARG"
     gene            6029..7075
                     /locus_tag="PK144_08175"
     CDS             6029..7075
                     /locus_tag="PK144_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF65740.1"
                     /translation="MSMSILLTLYLLPSVLVVLWAERRARLREGAAAAVRTTREAGTP
                     AVHRLPRQAAIPAPAPAPAMTTETLGRLVDGLNAYFSGGDGAGDEARLAIESDGSIRA
                     RYRGLQLHSRYAALHARSGVVSGRQAGVQVFTARGEAVSSWMPYARTLDKDQVVRLDR
                     LIRTLHVLNATHTPWNGRTLPLWLPVHPLHLRSLPGAFGAAFADILQRCGRTPADIVL
                     EFTDADRLPAERLTAAIEGFRSRGFGIACLLRGDDPDWLRRILALAPDALRLGPAHLE
                     AATGSHHERRRLAARITQARQAGVKVWLSGASTSDRIDLAMALGADGWLDEAVSADLA
                     LPDTGTAGLPLAAR"
     gene            complement(7088..9367)
                     /locus_tag="PK144_08180"
     CDS             complement(7088..9367)
                     /locus_tag="PK144_08180"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNF65741.1"
                     /translation="MSDEVLKQLALQYHRAPTPGKIEVVPTKPLATQRDLALAYAPGV
                     TAACEAIARDPAEVANLTARGNLVAVVTNGSSVLSLGPLGPLAAKPVMEGKGVLFKKF
                     AGIDVFDIELDENDPSMLVEVIARMEPTFGGILLEDIKAPECFEVEQRLRERMKIPVF
                     DDDQHGTAIVVAAALLNGLRVVGKHISEIRLVCAGVGASAVACLDLLVRLGLPAARIS
                     VIDSEGVLHSGRAERLDPYKAKWVRDTAERTLAGLLKDADVFLGLSAPEVLHPSDLRG
                     MAPNPLILALASPEPEIRPEHAHLVRSDAIVCTSLPDHPNHVNNVLCFPYMFRGALDC
                     GASTINEAMTIACVHALADLAMEDPDEIAMAAYGEHTLSFGPNYLIPKPFDPRLILEL
                     PVAVARAAMESGIATRPIEDFDAYREELSRHVYRSGLIMKPVFERAKADPKRVVYCDG
                     EDDRVLRAVQMVVDEGLGRPILIGRSERIRERIEQLGLHLYIGEDFELIDQRDHIYFD
                     PCWKEYHRLLERQGITEANAQHRVRTRPAVLGALLVKLGHADTMITGLQARYRRQLEY
                     LVDVFGLPEGVAAPAAISLLLGPRGPLFLCDTHVTPKPSASAIASMALMAADQVRRFG
                     LVPKLALLSHSNFGSDDTRSSRRMAKALTLLREQAPELEVEGEMQGDTALSEVLRRRL
                     FPHSRLSGTANLLIMPSLDAGNIAYNLLRMVTDSVAIGPMLMGLALPGHVLTPAATVR
                     RVVNMTAVAVVDAQINPGR"
     gene            complement(9489..9902)
                     /locus_tag="PK144_08185"
     CDS             complement(9489..9902)
                     /locus_tag="PK144_08185"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF65742.1"
                     /translation="MSDPLPSLQPEGEPAIRTIAMPADANPAGDIFGGWLMAQMDLAA
                     GNVAARRARGRCVTVSVDHIAFLSPVYVGDEVSLHARVVRVGRTSMTIHVEAWRRSRD
                     GDDRVRVTEANFTFVALDQGRRPRVLPPPTAQDPC"
     gene            complement(9998..>11062)
                     /locus_tag="PK144_08190"
     CDS             complement(9998..>11062)
                     /locus_tag="PK144_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNF65743.1"
                     /translation="AHPVAQGAMELQLEPGRIVLDVHASMEQVLVQAAYGPAATATLP
                     ERIRLHGEYLRAHLRLAADDVALQAGPAEALAARPGRAAWRLVFPLPAGSRPARLRLW
                     QDVLREIEYAPGNPWEASYVLRVSGAGAGEGGLLASAQTLSLSLSPAPSAPAAAPPAP
                     AVPVAQWIAHGLRHILAGYDHLLFVAALTLGARRLGELVWLVGAFTLAHTLTLALSAL
                     DVIRLPSGLVEPMIAASIVAVALLNVFGSTDARTPHRRLLCAFGFGLFHGLGFAGGLL
                     EAFADLGGGAAVTAILAFSVGVEGGHLAVVLPLWALLAALRHRRAGSDPRALRRGASV
                     AISLAGLLYLAAALGHGPLA"
BASE COUNT         1834 a         3707 c         3788 g         1733 t
ORIGIN      
        1 ccggtccttt cacggtcgag agcctgagcc cgcaccgcat cctgggcgtc gatgaagacg
       61 acgaactcat cgacagcctg cgcgagcctg atgccgaata tggcgccaga caatccttcc
      121 cgcagatgat tctggaaaac ctgaaaaccg cgggcgtgca gcaggcgcac aaggaagacc
      181 gcatcagttt caccgcgctg atggcctggc cgggcgacat ggtatgcgcc gaggggcgct
      241 atctcgaagc cgagggcgtg gagaagcgcg ccgcgatctt catcggccca gaattcggca
      301 ccgtgcaacg cgccgatctg gtcgaggccg cgcgcgaagc cgccgatgcc ggtttcgacg
      361 tgctgattgc ctgcgccttc aactacgagg cgcataccac cgactttgcc aggctcggca
      421 agctgccggt gctcaaggcg cgcatgaacg ccgacctgca catggccgaa gacctgaaga
      481 acaccggcaa gggcaacctg ttcgtgatct tcggcgagcc ggacatcacg ctcctgcctg
      541 aaggcgatat gctgcgcgtg aaggtgaatg gcgtcgacgt gttcaagccg cagaccgggg
      601 aggtcatcag cgacggcgcg gacggcatcg cctgctggtt catcgacacg gactacaacg
      661 aggaaagctt cttcgtgcgc cacgcctact ttctcggcgc caacgatccc tacagcgcct
      721 tgaagaccac gctcaaggcc gaaatcgacg ccgacgcctg ggcctcgctc aacagcgaca
      781 cttcgcgccc gttcaggaaa ccgaagtccg gacgcattgc cgtgaaggtc atcaatcacc
      841 tgggagatga ggtgatgaag gtattccggg tggcatgatg cagtctgaaa ggagacaccc
      901 atgaatacag ccgacgccat ccgcctgctg gccgagagca agccgatgct ggccgaacgc
      961 ttcggggtga aggatctggc gctgttcggc tcgaccgcgc gtggcagtgc cggcgctgcc
     1021 agcgatgtcg acgtactggt ggcattcgac ggcccggcga cctcggcacg ctatttcggc
     1081 gtgcagttct atctggaaga cctgatgggc tgtgcggtcg atctggtcac cgacaaggcg
     1141 ctgcggccgg aattgcggcc cttcgtcgag cgtgaggcca ttcatgtctg ataccttgca
     1201 gagggaatgg cggttctaca tcgacgacat gatcgatgcc tcccggaagg tgctggctta
     1261 cacagcgggc ctcgatcagg cggggtttgt agccagcggg ctccattacg acgccagcgt
     1321 gcgcaatatc gagatcatcg gcgaggcagc cacccacgtt cccgaggacg tgcgcaacgg
     1381 ccacccgcta attccctggc gccagatcgt cgccacgcgc aatcggttga tccacggcta
     1441 tctgggcatc gacaacgaca cgctgtggag catcatccgg gacgacatgc cggatttgct
     1501 ggaagcactg gtcaggttga aggaaagtca gggctgagca tggaattccg tatcgccgac
     1561 acgttcaccg acagcctggg acgcctgacc ggcgacgagc agaagctcgt caagacgacg
     1621 gcgttcgatc tgcagctgaa tcccgccagc cccggcatga atttccacaa actggatcgc
     1681 gccagggacc agaacttctg gtcggttcgc gtcggcagcg atattcgtct gattgttcac
     1741 cgcagcgaaa ccagcctgtt gctctgctac gtcgatcacc acgacaaggc ttacgaatgg
     1801 gcggagcggc gcaagctgga gacgcatcca aaaaccggcg cggcgcaact gatcgaaatc
     1861 cgcgagacgg tgcaggaaat cgtcgtgccg gtctatgtgc agagtgagtt gccgttgccg
     1921 gcaacgcctg cgaaaccagc ccctgcagcg aagctcttgt tcctggcggt gtccgaagac
     1981 gaactgctga gttacggtgt tccgccggag tggctgcccg atgtgaagca agccaccgag
     2041 gacagcctgc ttgctttagc cgatcacttg cctgccgaag ctgccgaagc acttctggaa
     2101 cttgcgacag gcggcaaacc tcgcttgccg accccgtcga cggtgaatcc gttcgaccac
     2161 cccgacgcgc aacgccgatt ccgggtgatg agcaatgtcg aggagttgca gagcgctctc
     2221 gattatccat gggagaaatg gacggtcttc cttcacccgg aacagcgcca atgggtagag
     2281 cgcgatttca acggaccggc acgcgtcgct ggttcggcgg gcaccggcaa gaccattgtg
     2341 gccctgcacc gcgccgcgta cctggcacgc cagcatcccg atgcgcgtgt attgctgacc
     2401 acgttttcga acactctggc caatgccctg caatcgaagc tgaatcgact gctgacccac
     2461 gagcctcgac ttgccgagcg gatcgacgtg cactcgctcg aagccatcgg gctgcgattg
     2521 cacaagaccc aggtcggcgt tgcaaagctt gcgtcccgcg aggaaattct ccagctcgtg
     2581 ggcgatgccg ccacggcagt accgggacac aggttcggtc gccacttcct gctgaccgaa
     2641 tgggaacagg tggtcgacgc ctggcaactc gatcattggg aggcatatcg cgacgtggtg
     2701 cgccttggcc gcaaaacccg gctgccggaa gcgcagcgcg cagttctgtg ggcgattttc
     2761 gagcacgtcc gcgccgggtt gcgcgacaaa ggtctggtaa cggcgtccgg actattcact
     2821 gcgttggcgc acgcactgaa agcacgcggc aaccctgtct tcgattttgc tgtcgtcgat
     2881 gaggctcagg atattggagt cgcccatctg cgatttcttg ctgccctcgg tggccaattc
     2941 ggtggcggcg aaggcgcggt tcgtccgaat gcgttgtttt ttgcaggaga cctcgggcag
     3001 cgcatcttcc agcagccgtt ttcgtggaag tcgcttggtg tcgatgtgcg cggacggtcc
     3061 cgcacgctgc gcgtgaatta tcgtacatcg caccagattc gcagccaggc agaccgattg
     3121 ctcggcccgg gggtgactga tgttgatggc aataccgaag atcgcagcga taccgtgtcc
     3181 gtgttcaatg gtccgccgcc acgcatccag atattcaacg atgctgaaaa agaaagcgcc
     3241 gccatcgggc gctggcttgc cgagtgtgga agcgccggca tcctgccgca cgagattggc
     3301 gtgttcgtac gatcagccgc gcaaatcgat cgggccgagg acgccgttcg cctcgccgcg
     3361 ttgccttacc agattctgga tgagcatgtg gcgacggagc atgaccacgt atcgatcagc
     3421 accatgcatc tggtaaaggg tctggagttc cgcgcagtgg ccgtgatggc ctgcgacgat
     3481 gaagtgatcc cgctgcagga gcgtatcgaa acggtcggcg acaatggcga tttgcaggaa
     3541 gtttatgact ccgagcggca gctgctttac gttgcctgca cgcgagcacg ggatcggttg
     3601 ctggtcactg gaacaaagcc gtcgtctgag ttcctggacg atctgaccgg tggttgaatt
     3661 tcgttctgcc cgacccgggt tgcggacgtc agaaagcctg tccatcgaca gggcgtgccg
     3721 gaaaaacccc tgatccccgg cccttccccc aggacgggca tgagtatcta cacatctcca
     3781 gcggccctgc ggatgccgga ataaccggcc ggtagatcat cgataaattc aagtcgttga
     3841 ccgtaaagca aatcatgcaa aaccgcaggg caggtcaggc gcgcaccgga cccggtatcc
     3901 cgttacctca gaacggactg cgcgccgtaa cccgccttcg ggatgcccgg tgcgcctggc
     3961 ccgaaggcgg gttacggcgc gggtgaggcc gtgcaaatca ttgtcaccat gggtcgcgcc
     4021 taacccgccc tacgtcagtg ccatgcggtg acttgtgcag atacccatgc cagacaggga
     4081 atggtgaaaa accacgcatt tacgcgggat cggtatcagc cccgcgccgc ctcgccgccg
     4141 ccgtccgcat cgttatcggc cgccgcaacc tcgcccgcca cccgaaagcc atcccgcccg
     4201 gcccgcttgg ccgcatacat cgccgcatcg gcggccgcga acaaggcctc ggagttctcg
     4261 ccgtgctgcg ggtacagcgc gatgccgatg ctcgccgaca cgcgcgcctg ggtgccggcg
     4321 tgcggcaccg gccgggcgat tgcggcgatg accttgcgcg cgagccacag cgcgtcctcc
     4381 tcggccgtgt cgccgaggat cagcaccagg aattcatcgc cgccgaggcg tgcggccagg
     4441 tcggtgcgcc gggtctcggc actcagccgg gtggcgaccg tgcgcagcac catgtcgccg
     4501 gcgatgtggc cgagcgtgtc gttgacctgc ttgaagccgt cgaggtcgat gatcagcaag
     4561 gccagcggcc cgccgccggt cgcgatcacg tgcgccagcg tgtcgagcgc gcgccggcgg
     4621 ttggcgaggc cggtcagcgg gtcgtgcgcg gcctcgtggg caatctccag atgcgcggcg
     4681 tgcagcgtgt cgagcatgcg gttgatcgcg cgcccgagcc ggccgagttc gtcctgcccg
     4741 gcatcgtgga tgcggtactg gcgggtgtcg cggctctgct cgaccgcatc gacgatcgcg
     4801 tcgatcgcgg cgatgcgcct gagcacccag ttctccagca gcagcagcac cagcagcagg
     4861 cagccgccgc cgagcacgag cagcgcggcg ctcaggtatt tctcggccgc cgccgcctgg
     4921 gcaccgagat ccggcagccg gtcgctgcgc agcaccgcca cggtgttgcc gaagggatcg
     4981 tgcaggcgca ggcgcccgac cagggcgccg tcctcgccac gcgccagcgc gatgtcggcg
     5041 tccgcctgcg gccagtcgag ggccggttcc tcgaagcgca gcggcaggtg cgtgacctgg
     5101 ctgaggctgg cgagcagcgt tgcgtcgagc cgccggccga gcaccagcgc gccgtgggcc
     5161 ggcccctgac gctggctggt cagcaccggc cgcacggcga tcaggtaggc atgcgcgccg
     5221 agacgcacgc tggcgcgcag gacttcgctt tcgccgcggg ccagcgccgg cagctcgggc
     5281 agttgcgcca gggcctgcac cgcttcgggc ggcagccgcc gcagctgcgg cggaatgcgc
     5341 aggccgtcgt gttcgcctgc ctcgtcctgc gacggtgcag gtccgccctc gctgcccggc
     5401 gggcgggtgc gcaacggccg ctgcacggcg aacaccggcc gggcgctgtc cgcatacagc
     5461 gcgaacaggt cgacgcggat cgcatcgagg ccgccgtcgg tgaggttcac gtcgaggtat
     5521 tcctgcgccg gcgccagcgc gaagcgccag gtctcgtccc aggccgacca gtcgatggtc
     5581 acgtcatcga gcgcgcgcag ttcgtagtcg atgagctggc gcacgctttc gaggtgggtg
     5641 cgcgcctgcg ccgtttcggc ctcccggtag ccgcgctgca gttcctcgcg accgagaccg
     5701 tacagggcag ccagcaggct gccgagcacg acggtggtcg cgagcaggat gcggttgcgc
     5761 agactcattg cgcgtcaggg ccttgatgca gggtcgggtt gtccatgggc gtcgcagtat
     5821 gccggcttgc accccggcct gcacggctgg ccggtccggt tcccgggatc ggttccggag
     5881 gcattcgcgc agtctttaat aactaaatat tattataata atattttata ttatttattt
     5941 gaatatcact ccggccgtat cttggctccg cggctccggc cgaaagcacg aaatccgccc
     6001 gtccggttcc ggcaagggag aactgagcat gtcgatgtcg atcctgttga cgctgtacct
     6061 gttgcccagc gtgctcgtgg tgctctgggc cgagcggcgc gcccgtctgc gcgagggcgc
     6121 tgccgcagcc gtgcggacga cccgcgaggc cggcacgccg gccgtgcacc ggctgccgcg
     6181 tcaggctgcg attccggcac cggcaccggc accggccatg accaccgaaa cgctgggccg
     6241 gctcgtcgat ggcctgaacg cctatttctc cggaggtgac ggggccgggg acgaggcgcg
     6301 cctcgcgatc gaaagcgatg gcagtatccg cgcccgctac cgcgggctgc agctgcacag
     6361 ccgctacgcc gcgctccatg cccgcagcgg cgtggtcagc ggccggcagg ccggcgtgca
     6421 ggtcttcacg gcacgcggcg aggcggtgtc gagctggatg ccgtatgcac gcacgctcga
     6481 caaggatcag gtcgtgcgcc tggaccggct gatccgcacg ctgcacgtgc tgaacgccac
     6541 gcatacgccg tggaacggcc gcacgctgcc gctgtggctg ccggtacatc cactgcacct
     6601 gcgttcgctg ccgggggctt tcggggcggc gtttgccgac atcctgcagc gctgcgggcg
     6661 cacgccggcg gacatcgtgc tcgaattcac cgatgccgac cggctgccgg cggaacggct
     6721 gacggccgcg atcgaaggct tccgcagccg cggcttcggc atcgcctgcc tgctgcgcgg
     6781 cgacgatccg gactggctga ggcgcatcct ggcgctggcc ccggatgcgc tccggctcgg
     6841 cccggcgcat ctggaagcgg ccacgggttc acatcacgaa cgccggcggc tcgccgcccg
     6901 catcacgcag gcccggcagg ccggcgtgaa ggtctggctg tccggtgcct cgaccagcga
     6961 ccggatcgat ctggccatgg cgctcggggc cgacggctgg ctcgacgagg ccgtgtccgc
     7021 cgacctggcc ctgccggaca ccggcacggc cggcctgccg ctggccgcac gctgagcgcc
     7081 cggcgcctca gcgccccgga ttgatctgcg cgtcgaccac ggccaccgcc gtcatgttga
     7141 cgacgcggcg cacggtcgcc gccggcgtca gcacgtggcc gggcagcgcg aggcccatca
     7201 gcatcgggcc gatcgccacc gagtcggtga ccatgcgcag caggttgtag gcgatgttgc
     7261 cggcatcgag cgaaggcatg atcagcaggt tggcggtgcc cgagagcctg gagtgcggga
     7321 acagccgccg gcgcagcacc tcggagagcg ccgtgtcgcc ctgcatctcg ccttccactt
     7381 cgagttccgg tgcctgctcg cgcagcagcg tcagcgcctt ggccatgcgc cgcgacgagc
     7441 gcgtgtcgtc actgccgaag ttcgaatgcg acagcagcgc gagcttcggc accagaccga
     7501 agcggcgcac ctgatcggcg gccatgagcg ccatcgacgc gatcgcgctc gcgctcggct
     7561 tcggcgtcac gtgcgtgtcg cagaggaaca gcggtccgcg cgggccgagc agcaggctga
     7621 tcgccgccgg tgcggcgacg ccctccggca ggccgaacac gtcgaccagg tattcgagct
     7681 ggcggcggta gcgcgcctgc aggccggtga tcatcgtgtc ggcgtgcccg agcttgacga
     7741 gcagcgcgcc gagcacggcc gggcgcgtgc gcacgcggtg ctgggcgttg gcctcggtga
     7801 tgccctgacg ctccagcagc cggtggtatt ccttccagca cggatcgaaa tagatgtggt
     7861 cgcgctggtc gatcagctcg aagtcctcac cgatgtacag gtgcaggccg agctgctcga
     7921 tgcgctcgcg gatgcgctcg ctgcgcccga tcaggatcgg ccgcccgagc ccctcgtcga
     7981 cgaccatctg cacggcgcgc agcacgcggt cgtcctcgcc gtcgcagtag acgacgcgct
     8041 tcggatcggc cttcgcgcgc tcgaacaccg gcttcatgat caggccgctg cgatagacgt
     8101 ggcgcgagag ctcctcgcgg taggcgtcga agtcctcgat cggccgcgtc gcgatgcccg
     8161 actccatcgc cgcccgcgcg accgcgaccg gcagctccag gatcaggcgc ggatcgaagg
     8221 gcttcgggat caggtagttc ggcccgaagc tcagcgtgtg ctcgccgtag gcggccatcg
     8281 cgatctcgtc ggggtcctcc atcgccagat cggcgagcgc gtgcacgcag gcgatggtca
     8341 tcgcctcgtt gatcgtgctc gccccgcagt cgagcgcgcc gcggaacatg tacgggaagc
     8401 acagcacgtt gttcacgtgg ttcgggtgat ccggcaggct ggtgcagacg atcgcgtcgc
     8461 tgcgcaccag atgcgcgtgc tcggggcgga tctcgggctc ggggctcgcg agcgcgagga
     8521 tcagcgggtt cggcgccatg ccgcgcaggt cggacggatg cagcacctcc ggcgccgaca
     8581 ggccgaggaa cacgtcggcg tccttcagca gccccgcgag cgtgcgctcg gcggtgtcgc
     8641 gcacccactt cgccttgtac gggtcgagcc gctcggcgcg gccgctgtgc agcacgccct
     8701 cggaatcgat gacgctgatg cgcgccgccg gcaggccgag ccgcaccagc aggtcgaggc
     8761 aggcgaccgc cgaggcgccg acgccggcgc agacgaggcg gatctcggag atgtgcttgc
     8821 cgaccacgcg caggccgttg agcagcgccg cggcgacgac gatggccgtg ccgtgctggt
     8881 cgtcatcgaa caccgggatc ttcatgcgct cgcgcaggcg ctgctcgacc tcgaagcact
     8941 ccggcgcctt gatgtcttcg agcaggatgc cgccgaaggt cggctccatg cgcgcgatga
     9001 cctcgaccag catcgacggg tcgttctcgt cgagttcgat gtcgaacacg tcgatgccgg
     9061 cgaacttctt gaacagcacg cccttgcctt ccatgaccgg cttcgcggcc agcggcccga
     9121 gcggcccgag gctcagcacc gacgagccgt tggtgacgac ggcgaccagg ttgccgcgcg
     9181 cggtgaggtt ggcgacctcg gccggatcgc gggcgatcgc ctcgcaggcg gcggtgacgc
     9241 cgggcgcgta ggcgagcgcc aggtcgcgct gcgtggcgag cggcttggtc ggcacgacct
     9301 cgatcttgcc gggcgtcggc gcccggtggt actgcagcgc aagctgcttc aggacttcgt
     9361 cgctcatggc ggcgggacct ctgcgaatac ggcgactgcg atgaatgcgg cgaatacggc
     9421 gaaaaatccg tcgaaactcg tcgaaatccg tcgggatgcg cccggaacag gacaggaacg
     9481 tcagcggctc agcacgggtc ctgcgcggtc ggcggcggca gcacgcgcgg ccggcggccc
     9541 tgatcgagcg cgacgaaggt gaagttggcc tcggtcacgc gcacgcggtc atcgccgtcg
     9601 cgcgagcggc gccaggcctc gacgtggatg gtcatcgacg tgcgcccgac gcgcacgacg
     9661 cgcgcgtgca ggctgacctc gtcgccgacg tagaccgggc tcaggaaggc gatgtgatcg
     9721 accgacaccg tcacgcagcg gccgcgggcg cggcgcgcgg cgacgttgcc ggcggcgaga
     9781 tccatctgcg ccatcagcca gccaccgaag atgtcgccgg ccggattggc atcggccggc
     9841 atcgcgatcg tgcggatcgc gggttcacct tcgggttgca gggaaggaag cggatcggac
     9901 atggacgacg gaaccggcag cggcggcggg gccgcacagc gtagcgcgag cctcggcaat
     9961 gctcatccgc gatgtgctgc accctgcgcg atggatttca cgccagcggg ccgtgcccga
    10021 gcgcggcggc cagatacagc agccccgcga gcgagatcgc gaccgaggcg ccccggcgca
    10081 gggcgcgcgg atcgctgccg gcgcgccggt ggcgcagcgc cgcgagcagc gcccacagcg
    10141 gcagcacgac cgcgagatgc ccgccctcga cgccgacgct gaacgcgagg atggccgtga
    10201 cggccgcacc gccgccgaga tcggcgaagg cctccagcag cccgccggca aagccgaggc
    10261 cgtggaacag cccgaagccg aacgcgcaca gcagccgccg gtgcggcgtg cgcgcatcgg
    10321 tgctgccgaa cacgttcagc agcgcaaccg cgacgatgct ggcggcaatc atcggctcga
    10381 cgaggccgga cggcagccgg atcacatcga gcgccgacag cgcgagcgtc agcgtgtgtg
    10441 cgagcgtgaa cgcaccgacc agccagacca gctcgccgag gcgccgcgca ccgagcgtca
    10501 gcgccgcgac gaacagcagg tggtcgtagc cggcgaggat gtgccgcagg ccgtgggcga
    10561 tccactgcgc caccggcacg gccggggccg gcggtgccgc ggccggagcg gacggcgccg
    10621 ggcttaatga caggctcagc gtctgcgccg aagccagcag cccgccttcg ccggctccgg
    10681 ccccggacac gcgcagcacg tagctcgcct cccacggatt gcccggcgcg tactcgatct
    10741 cgcgcagcac gtcctgccag agcctcaggc gtgccggccg gctgccggcc ggcagcggga
    10801 acaccagccg ccacgccgcc cgccccggcc gcgccgccag ggcttcggcc ggaccggcct
    10861 gcagggccac gtcatcggcc gcgagcctca ggtgcgcgcg cagatattca ccgtgcaggc
    10921 ggatgcgctc gggcagcgtg gccgtggccg ccgggccgta ggcggcctgc accagcacct
    10981 gctccatcga cgcatgcacg tcgagcacga tgcggccggg ttcgagctgc agttccatcg
    11041 cgccctgcgc gaccggatgc gc
//
LOCUS       DAOQIE010000104        11059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2935_length_11059_cov_7.507088, whole genome shotgun sequence.
ACCESSION   DAOQIE010000104 DAOQIE010000000
VERSION     DAOQIE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2935_length_11059_cov_7.507088"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1336
                     /locus_tag="PK144_08195"
     CDS             281..1336
                     /locus_tag="PK144_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65744.1"
                     /translation="MTADPEKTSAFHEAPAAAGATPAGTTLPASAAWRATLDAAVAAA
                     GQAWLAGRRTRVAPFARRHFGWRGAWRLNRRAFGKDLLRAPVNVLWAAPHLLLQLGAR
                     AGRKAGLKRTAERLEQLPAGWRTDVERELEWLIRTELLELPCTQPGRTSSRDTLFEAV
                     LAQPAVAGALVEALQALDALGQRTELHGRLADFLRQYTDTRAAAADLANSLLALAAGA
                     AAFQSFTPGTVAMGAAAAAGIAQQLAVANFTLGPTLGALWYGVFPASASAGLVAATTG
                     GLIAALGVLAACSGLVTDPLQQALGLHERRLHKLLDALDAELAGQSPRGPALRDAYVA
                     RVFDLLDLIQTAAKALR"
     gene            1435..1662
                     /locus_tag="PK144_08200"
     CDS             1435..1662
                     /locus_tag="PK144_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNF65745.1"
                     /translation="MDIAERITIEPGKRGGRACVRGMRIAVADVLGWLAQGMTVAEIV
                     ADYPELEPEDVSACLTFAAERERGTVTVHAA"
     gene            1659..1991
                     /locus_tag="PK144_08205"
     CDS             1659..1991
                     /locus_tag="PK144_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNF65746.1"
                     /translation="MKLLLDQNLSRRLVPVLQAAYPGSTQVALLGLACASDRDIRDHA
                     AQHGYVIVTKDADFRDMAVLHGVPPRIIWLRSGNGPRQAVAERLLAAHPMLEAAFADP
                     ATVCIELC"
     gene            complement(2075..4747)
                     /locus_tag="PK144_08210"
     CDS             complement(2075..4747)
                     /locus_tag="PK144_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF65747.1"
                     /translation="MSVGKPQQVRATVASTLAFTVCFAVWTIFSIIGIQIKKQLGLSE
                     TEFGLLVGTPILTGSLIRLVLGVWTDQIGGRVVFLWVMLSAAIATWLLTYAQSYQMFL
                     VAALGVGIAGGSFAVGIAYVSKFFDTRSQGTALGVFGAGNVGAALTKFVAPFVMVAYG
                     WQTVAQVWAAAIAITGVLFYLCTEDDPSLKARRATGTKPQSFLQQMEPLKHLQVWRFS
                     LYYFFVFGAFVALALWLPRYLTGAYGMDIKEAGMVAAMYSIPASLFRIVGGWLSDKYG
                     ARTIMYWTFIVSAICTLILSYPPADFVVRGIHGPISFHLSTGVGGFIAIAFVLGFFMS
                     LGKAAVYKHIPVYYPQHVGSVGGVVGMIGGLGGFLLPITFGMLNDVTGIWQSCFMLLF
                     VIVTVSLLWMHFSILRMERRVVPQLKDLPQDLPELVSESPMTLTEWQPEDKDFWENKG
                     RKIAQRNLWISIPNLLLAFAVWMVWSVVVVNLPNLGFKYSANQLFWLAALPALSGATL
                     RIFYSFIVPIFGGRRWTTISTASLLLPCIWIGFAVQNPETPYLVMLILALLCGFGGGN
                     FSSSMANISFFFPKSQKGTALGLNAGLGNLGVSTVQFVVPLVITASVFGGLGGEPLTW
                     TKGDVTKQMWLQNAGFIWVPFIIASAIAAWFGMNDIASAKSSFKDQAVIFKRKHNWLM
                     CWLYLGTFGSFIGFSAGFPLLTKTQFPDVNPTQYAFLGPLVGALIRPVGGWLSDKLGG
                     ARVTFWNFIAMIAAVFGVMAFLPHAGQGGSFAGFLLMFMVLFVTTGIGNGSTFRMIPV
                     IFMTERQRAAAGQGEAAQEQAIKDANKEAAAVLGFSGAIGAYGGFFIPKSFGTSIELT
                     GSATAALIGFVAFYVSCIVVTWWWYSRKGAEAPC"
     gene            5191..7059
                     /locus_tag="PK144_08215"
     CDS             5191..7059
                     /locus_tag="PK144_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF65748.1"
                     /translation="MTLPPLPPLRDSLLWRMGLLLGGIALLALIGMSNALLTAVASAG
                     DAAAINIAGSLRMQSYRIATRLGAAAGPAAVRDELDEFERRFTHPQLLHAIPADAGDA
                     RRLDYSALATQWHERLRPLALELGSGTARPERLRTALAEIDDFVAGIDGFVGRLETHA
                     EAKLTALRWVQGAILLATLLLAVLAWRSVRLRLLPPLRELLGLADRVRRGDFIGRAEH
                     VGRDELGVLAQAFNAMAEDLSKMYADLESRVEAQTHALRRSNRSLELLYATTRRLNEA
                     PPTAATLTAVLAEVERYTGARGSSLCLVDAQSLKLQQKISTHPDFPERCRKPDCGSCV
                     GDGSSSEPAHLPQTLSVPVQELGEYYGVLLLEAPGIGVEDATQVQLLETVARHIASTL
                     RATRQEVSKRRLALLEERNVIARELHDSLAQQLSYLKIQVSRLQSALPDGQPAAVGEI
                     LAELREGLSSAYRELRELLTTFRITMAEDGLQPALERALREFGTRGNLATVLDDRLNG
                     HPLSPHQEIHVLQIVREALSNIVHHARARTARVTLAHVDGRMQVTIEDDGVGLSDNPE
                     RERHYGLTIMRERTRSLHGELQLGRSTHGGTRVALDFPATHAEPPTDTSAEESLHV"
     gene            7052..7711
                     /gene="narL"
                     /locus_tag="PK144_08220"
     CDS             7052..7711
                     /gene="narL"
                     /locus_tag="PK144_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HNF65749.1"
                     /translation="MSDAPQTLLVIDDHPLLRKGVSQLVAMEPEMRLVGEAADGSEGL
                     KLARQLEPDLILLDLQMKGMNGLETLKAIKALDTDSRVIMFTVSDSEDDILAALQAGA
                     DGYLLKDMEPEEMLANLRMAARGRLVLSGPVTELLARSLRNESSSGALPAADLTDREH
                     EILLHIAEGRSNKLIARALDITEGTVKVHVKHLLKKLNLRSRVEAAVWAVKSGIAQRH
                     G"
     gene            7919..8194
                     /locus_tag="PK144_08225"
     CDS             7919..8194
                     /locus_tag="PK144_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65750.1"
                     /translation="MSGPNRKLDRNDRYFRLPSAGSPVRLPAAVAHGAQPRLEDWCLI
                     AGDRLSGRVYDDPRFEDGEHITTSPVLLLSNHAAATCTTHYRLGRPA"
     gene            complement(8258..9109)
                     /locus_tag="PK144_08230"
     CDS             complement(8258..9109)
                     /locus_tag="PK144_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNF65751.1"
                     /translation="MRQLSPAGQQAVDDISRRHGFSSDAVRAMLDAVINGNGTMAQFS
                     HHEFGGSGQWMAGGMLMLGDMFNHGLKSRVDALCQELSTLVANQPGLLQTGSFQSQSQ
                     GSGHNDSYQQQGNWNPPSGQSGTGSSLFTPQADWWGPELRWPNSTGAQNGVRYAYFAQ
                     AHRLAIETGGHVTIYDTLDHQIGGVSQQQGSSGTLSFTSQYGTVNLSSLPVISGGGRQ
                     PDAWSPSSSPSSPAPMNSPAPAAASGTASESDIFATIEKLAGLRAKGFISDDEFNAKK
                     AELLSRL"
     gene            9430..10824
                     /locus_tag="PK144_08235"
     CDS             9430..10824
                     /locus_tag="PK144_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNF65752.1"
                     /translation="MPGRVEFSFGPQRRSARTDGSTVCRILILADLVGETLRPPIAER
                     QPMRVSAATLDALMARLAPRLELAAGSGLPALTIDFASLDDLHPDQLLARVPLFADID
                     ASARRLAVQPQAAPPVPAGQPASPAEDFAALLGGSVGRSAAGSDMQQKAEDFVRRLLA
                     GTSSATPVPDPRQTVLDAACALARTQALRALLHHPDFQRLESAWRSLAMLVAGVDNDA
                     GVEVWVLDIGRCELCADLAADLPILANLILQSAEQTGEWGLLVSTETFGASAEDVTVL
                     AAFGAIAGDCGATVLAGMDPAITIDPAGFAADAISAARWSAVRALVGSNRIALAAPRL
                     LLRRPYGARSDPLSSAAFEEVDACPAHEDFLWGSGGVGLALLLGQAWEQNEGWEFDPD
                     QWRELDDLPMALLARNGERELVPCAERWLSDVAAQTLDGLGATVLLSRRDRNQASVLR
                     FRSLAGALRGRWND"
BASE COUNT         1815 a         3766 c         3792 g         1686 t
ORIGIN      
        1 acgctcgctg ctggcccgcc agcgcgccgg cgccagaccc gcggtggcgc tgcaatctgc
       61 agtgcactga tccggcagtt gattttcatc cttcccccac cgggggaagg gccgtggatg
      121 agggggcggt atggcacgga atcatggcgg gtgatggtgc tctgatcgct gccctgcgga
      181 ccgggatgcc tgccgatccc ggccggcagc gggcatcccg ctgcggcatc atccggccag
      241 ggctgcgaac cgcagcgcca tgcgccgggt cgatatccgg atgactgccg atcccgaaaa
      301 gacctctgcc ttccacgaag cccccgctgc ggccggcgcg actccggccg gcacaacgct
      361 gccggccagc gctgcctggc gcgcgacgct cgatgccgcc gtcgccgcgg ccggtcaggc
      421 ctggcttgcc gggcgccgca cccgtgtcgc gccgttcgcg cgccggcatt tcggctggcg
      481 cggcgcctgg cggctgaacc ggcgggcctt cggcaaggat ctgctgcgtg cgccggtcaa
      541 cgtgctgtgg gccgcaccgc acctgctgct gcagctcggg gccagagccg ggcgcaaggc
      601 cgggctgaaa cgcacggccg agcggctgga gcagctgccg gcgggctggc gcaccgatgt
      661 cgagcgcgag ctggaatggc tgatccgcac cgagctgctg gagctgccct gcacgcagcc
      721 cggacgcacg agttcgcgcg atacgctgtt cgaggccgtg ctcgcgcagc cggccgtcgc
      781 cggcgcgctg gtcgaggcgc tgcaggccct cgatgcgctc gggcagcgga cggagctgca
      841 cgggcggctc gccgacttcc tgcgccagta caccgacacg cgcgcggcgg cggccgatct
      901 cgccaactcg ctgctggcgc tcgcggccgg cgccgcggcc ttccagtcct tcacgcccgg
      961 caccgtggcg atgggcgcgg cggccgcggc cggcatcgcg cagcagctcg ccgtcgccaa
     1021 cttcacgctc ggcccgacgc tcggcgcgct gtggtacggc gtgttcccgg ccagcgcctc
     1081 ggccggtctg gtcgcggcga ccaccggcgg cctgatcgcc gcactcggcg tgctcgccgc
     1141 ctgctcgggc ctcgtcaccg acccgctgca gcaggcgctc ggcctgcacg aacgtcgcct
     1201 gcacaagctg ctcgacgcgc tcgacgccga actcgccggc cagagcccgc gcggcccggc
     1261 gctgcgcgac gcctacgtcg cgcgcgtgtt cgacctgctc gacctgatcc agacggcggc
     1321 gaaggcgctg cggtgaaggc aggtgcagcg actgccctgt tggcggtggc tggacgttcg
     1381 ggatgggggg cgtaaagtcc ggaaaacaca cacgggcacc gcgagggcag accgatggac
     1441 atcgccgaac gcatcaccat cgagccgggc aagcggggtg gtcgcgcgtg cgtgcgcggt
     1501 atgcgcatcg cggtcgcgga cgtgctcggc tggctggcgc agggaatgac ggtggccgag
     1561 atcgtcgccg actacccgga gctggagccc gaggatgtgt cggcctgtct gacgttcgcg
     1621 gccgagcggg agcggggcac cgtgacggtg catgccgctt gaagctgctg ctcgaccaga
     1681 acctgtcgcg gcgactcgta ccggtgctgc aggccgccta cccgggcagc acacaggtgg
     1741 cattgctggg gctggcgtgc gcgagcgatc gggacatccg tgatcatgct gcgcagcatg
     1801 gctatgtcat cgtgaccaag gatgccgatt tccgggacat ggcagtcctg catggcgtgc
     1861 ctccacggat catctggctg aggagcggca acggcccgag acaggcggtc gccgaacggt
     1921 tgctggccgc tcatccgatg ctggaagccg cgtttgcgga tcccgcgacc gtctgcatcg
     1981 aactctgctg acggaacccg ctgcgcttcc cgccaaaaac cgacggcccc gtctccgggg
     2041 ccgtcgtcgt cgttgccggc gaatcagggg cggttcagca cggcgcttcc gcacccttgc
     2101 gcgagtacca ccaccaggtc accacgatgc agctcacgta gaacgccacg aagccgatca
     2161 gggccgcggt cgcgctgccg gtcagctcga tcgaggtgcc gaagctcttc gggatgaaga
     2221 agccgccgta ggcgccgatc gcgccggaga agccgagcac ggcggcggcc tccttgttcg
     2281 cgtccttgat cgcctgctcc tgcgcggcct cgccctgacc ggccgccgcg cgctggcgct
     2341 cggtcatgaa gatcaccggg atcatgcgga aggtcgagcc gttgccgatg ccggtggtca
     2401 cgaacagcac catgaacatc agcaggaagc cggcgaagct gccgccctgg ccggcgtgcg
     2461 gcaggaaggc catgacgccg aacaccgccg cgatcatcgc gatgaagttc cagaaggtca
     2521 cgcgcgcgcc gccgagcttg tccgagagcc agccgccgac cgggcggatc agcgcgccga
     2581 ccagcgggcc gaggaacgcg tactgcgtcg ggttcacgtc cgggaactgc gtcttggtca
     2641 gcagcgggaa gcccgccgag aagccgatga acgagccgaa ggtgccgagg tacagccagc
     2701 acatcagcca gttgtgcttg cgcttgaaga tcaccgcctg atccttgaac gaggatttcg
     2761 ccgaggcgat gtcgttcatg ccgaaccacg cggcgatcgc cgacgcgatg atgaacggca
     2821 cccagatgaa gccggcgttc tgcagccaca tctgcttggt gacgtcgccc ttggtccagg
     2881 tcagcggctc gccgccgaga ccgccgaaca cgctggcggt gatgaccagc ggcacgacga
     2941 actgcacggt ggatacgccg aggttgccga ggccggcgtt gaggccgagc gccgtgccct
     3001 tctgcgactt cgggaagaag aagctgatgt tggccatcga gctggagaag ttgccgccgc
     3061 cgaagccgca cagcagcgcg aggatcagca tcacgaggta cggcgtttcg gggttctgca
     3121 ccgcgaagcc gatccagatg cacgggagca gcagcgaggc ggtgctgatc gtggtccagc
     3181 gccggccgcc gaagatcggc acgatgaagc tgtagaagat gcgcagcgtc gcgcccgaca
     3241 gcgccggcag cgcggcgagc cagaacagct ggttggccga gtacttgaag ccgaggttcg
     3301 gcaggttcac gaccacgacc gaccagacca tccacaccgc gaacgcgagc agcaggttcg
     3361 ggatcgagat ccacagattg cgctgcgcga tcttccggcc cttgttttcc cagaagtcct
     3421 tgtcctccgg ctgccattcg gtcagcgtca tcgggctttc cgagaccagc tccggcaggt
     3481 cctgcggcag atccttcagc tgcggcacga cgcgccgttc catgcgcagg atcgagaagt
     3541 gcatccacag cagcgacacg gtgacgatca cgaacagcag catgaagcag ctctgccaga
     3601 tgccggtcac gtcattgagc atgccgaagg tgatcggcag caggaagccg ccgaggccgc
     3661 cgatcatgcc gaccacgccg ccgaccgagc cgacgtgctg cgggtagtag accgggatgt
     3721 gtttgtagac cgccgccttg ccgagcgaca tgaagaaacc gagcacgaag gcgattgcga
     3781 tgaagccgcc gacgccggtg gacaggtgga agctgatcgg gccgtggatg cccctgacga
     3841 cgaaatcggc cggcgggtac gacaggatca gcgtgcagat cgccgagacg atgaaggtcc
     3901 agtacatgat cgtgcgcgcg ccgtacttgt ccgacagcca gccgccgacg atgcggaaca
     3961 gcgaggccgg aatcgagtac atcgcggcga ccatgccggc ttccttgatg tccatgccgt
     4021 aggcgccggt cagatagcgc ggcagccaca gcgccagcgc cacgaaggca ccgaacacga
     4081 aaaagtagta cagcgagaag cgccacacct gcaggtgctt cagtggctcc atctgctgca
     4141 ggaagctctg cggcttcgtg ccggtcgcgc gtcgggcctt caggcttgga tcatcctcgg
     4201 tgcagagata gaacagcacg ccggtgatcg cgatcgcggc cgcccagacc tgcgcgacgg
     4261 tctgccagcc gtaggcgacc atcacgaacg gcgcgacgaa cttggtcagc gccgcgccga
     4321 cgttgccggc accgaacacg ccgagcgccg tgccctgcga gcgggtatcg aagaacttcg
     4381 acacgtaggc gatgccgacc gcgaacgagc cgccggcgat gccgacgccg agcgcggcga
     4441 ccaggaacat ctggtagctc tgcgcgtagg tcagcagcca ggttgcgatc gccgccgaca
     4501 gcatcaccca caggaacacg acgcgtccgc cgatctggtc ggtccagacg ccgagcacga
     4561 gccggatcag cgagccggtc aggatcggcg tgccgaccag caggccgaat tcggtctcgc
     4621 tgaggccgag ttgttttttg atctggatgc cgatgatcga gaagatcgtc cagaccgcga
     4681 agcagacggt gaaggcaagg gtggaagcca ccgtcgcccg cacctgttgg ggttttccga
     4741 ccgacatatg cagatacctc ggatgaccgg atcagagcgt ttcccggatc gtggaatccg
     4801 gactgagcgc catcgtcggt gtcgggcggc gggtgactgc ttgatgtgaa tcaatggctt
     4861 ccggtggggg taggctgaat tgccacgcgc gctgccccgt agggagtaaa acaggctcag
     4921 gcggcaagtg cctgaatgcg ctatcaatgc aggcccgggg cgccggaatc gcgcccgggc
     4981 ggacgcaggc ggtgccggct ccggctaccc cgtcctgccg ccggataccc cgcccgcgcg
     5041 ggcgggtgag agcgaaggtg gcagtgccgg agccggccgg tgcgccttga ccggcatcaa
     5101 ggtcgcggct ccgggccctg acgaggctgt gcagtccgca agccggcccc ttcgaccacc
     5161 gaccggattt cctgcgagag acccccgctg atgaccctgc ccccgctgcc gccgctgcgc
     5221 gattccctgc tctggcgcat gggcctgctg ctcggcggca tcgcgctgct cgcgctgatc
     5281 ggcatgagca acgcgctgct gaccgcggtc gccagtgccg gcgacgctgc ggcgatcaac
     5341 atcgccggct cgctgcgcat gcagagctac cggatcgcga cccggctcgg tgccgcggcc
     5401 gggccggctg ccgtgcgcga cgaactcgac gagttcgagc gccgcttcac gcacccgcag
     5461 ctgctgcacg cgatcccggc cgatgccggc gacgcgcgtc ggctcgacta cagcgcgctg
     5521 gccacgcagt ggcacgagcg gctgcggccg ctggcgctgg aactgggcag cggtacggca
     5581 cggcccgagc gcctgcgcac ggcgctcgcc gagatcgacg acttcgtcgc cggcatcgac
     5641 ggcttcgtcg gccggctgga gacgcacgcc gaggccaagc tgaccgcgct gcgctgggtg
     5701 cagggcgcga tcctgctcgc gacgctgctg ctggcggtgc tggcctggcg cagcgtgcgc
     5761 ctgcggctgc tgccgccgct gcgcgaactg ctcgggcttg ccgaccgcgt gcgccgcggc
     5821 gatttcatcg gtcgggcgga gcacgtcggt cgcgacgaac tcggcgtgct ggcgcaggcc
     5881 ttcaacgcga tggccgagga tctgtcgaag atgtacgccg acctcgaatc gcgcgtcgag
     5941 gcgcagacgc atgcgctgcg ccgctccaac cgttcgctgg aactgctgta tgcgaccacg
     6001 cgccggctca acgaggcgcc gccgaccgcc gcgacgctga ccgcggtgct cgccgaggtc
     6061 gagcgctaca ccggggcgcg cggttcgagc ctgtgcctcg tcgatgcgca gtcgctgaag
     6121 ctgcagcaga agatcagcac gcatccggac ttcccggagc gctgccgcaa gcccgactgc
     6181 ggcagttgcg tcggcgacgg cagcagcagc gaaccggcgc atctgccgca gacgctgtcg
     6241 gtgccggtgc aggagctcgg cgagtactac ggcgtgctgc tgctcgaagc ccccggcatc
     6301 ggcgtcgagg acgccacgca ggtgcaactg ctggaaaccg tcgcccgcca catcgcctcg
     6361 acgctgcgcg ccacgcgtca ggaggtctcg aagcggcgtc tggcgctgct ggaggagcgc
     6421 aacgtcatcg cccgcgagct gcacgattcc ctggcccagc agctctcgta cctgaagatc
     6481 caggtcagcc gcctgcagtc ggcgctgccc gacgggcagc cggccgcggt cggcgagatt
     6541 ctcgcggagc tgcgcgaggg cctcagcagc gcctaccgcg agctgcgcga gctgctgacg
     6601 accttccgca tcacgatggc cgaggacggc ctgcagccgg cgctcgaacg ggcgctgcgc
     6661 gagttcggca cgcgcggcaa cctcgccacc gtgctcgatg accgcctgaa cggccacccg
     6721 ctgtcaccgc atcaggaaat ccatgtgctg cagatcgtgc gcgaggcgct gtcgaacatc
     6781 gtccatcatg cgcgggcccg caccgcacgc gtcacgctcg cgcatgtcga tggtcgcatg
     6841 caggtcacga tcgaggacga cggcgtcggc ctgtccgaca atcccgagcg cgagcgccac
     6901 tacgggctca ccatcatgcg cgagcgcacg cgcagcctgc acggcgaact gcagctcggg
     6961 cgcagcaccc acggcggcac gcgcgtggcg ctcgacttcc cggcgaccca cgccgaaccc
     7021 ccgaccgata cctccgccga ggagtccctc catgtctgat gccccgcaaa ccctcctcgt
     7081 catcgatgac cacccgctgc tgcgcaaggg cgtatcgcaa ctggtggcca tggagccgga
     7141 aatgcgcctg gtcggcgagg ccgccgatgg cagtgaaggc ctgaagctcg cccgccagct
     7201 cgaacccgac ctgatcctgc tcgacctgca gatgaagggc atgaacgggc tggagacgct
     7261 gaaggcgatc aaggcgctcg ataccgattc gcgcgtgatc atgttcacgg tctcggattc
     7321 cgaggatgac atcctcgccg cgctgcaggc cggcgccgat ggctacctgc tgaaggacat
     7381 ggagcccgag gagatgctcg ccaacctgcg catggcggcg cgcggccgtc tggtgctgtc
     7441 ggggccggtg accgaactgc tggcgcgctc gctgcgcaac gaaagcagct ccggcgcgct
     7501 gccggcggcc gatctcaccg atcgcgagca cgagatcctg ctgcacatcg ccgaggggcg
     7561 cagcaacaag ctgatcgccc gcgcgctcga catcaccgag ggcacggtca aggtgcacgt
     7621 caagcacctg ctgaagaagc tcaacctgcg ctcgcgcgtc gaggcggcgg tctgggccgt
     7681 caagagcggc atcgcgcagc gtcacggctg aagcagcggc ccggtgcgca gggccggcgc
     7741 aggccatgga aacagcaagt cacggttttg atacgaaagc gtgaaacttc cggatgaacg
     7801 gaagttcaga atccgcattg attccacgtc cgccaaccct gcacagcgag gaacgcacca
     7861 gcgcccgggt acgggtccag gccaggtggg gcagacacga acagatgcgg aggccagcat
     7921 gtccggcccg aatcgaaagc tcgaccgaaa cgaccgttat ttccgcctgc cgtccgccgg
     7981 aagcccggtc aggctgccgg cggcggtggc gcatggcgcg cagccgcggc tggaggactg
     8041 gtgcctgatc gccggggatc gcctgagcgg ccgggtctac gacgatccgc gtttcgagga
     8101 cggcgagcac atcaccacca gtccggtgct gctgctcagc aatcacgccg cggcgacgtg
     8161 cacgacgcac tatcgcctgg gccggcccgc ctgaatccat tccttcgcaa gcacaccggt
     8221 cccagcagac agccggcgac gttccggtcg ccggatctca gagccgtgac agcagttctg
     8281 ccttcttggc gttgaattca tcgtcgctga tgaaaccctt cgcgcgcagg ccggcgagtt
     8341 tctcgatggt cgcgaagatg tcggattcgc tggcggtgcc ggatgcagca gccggtgccg
     8401 gcgagttcat cggcgcaggg gaggacggtg acgatgacgg ggaccaggca tccggctgcc
     8461 tgccgccgcc ggaaatcacc ggcaggctgg agaggttcac ggtgccgtac tggctcgtga
     8521 agctcagcgt gccggagctg ccctgctgtt gcgagacgcc gccgatctgg tgatcaagcg
     8581 tgtcgtagat cgtcacgtgg ccaccggttt cgatggcgag gcggtgggcc tgcgcgaagt
     8641 acgcatagcg cacgccgttc tgcgccccgg tgctgttcgg ccagcgcagc tccggacccc
     8701 accagtccgc ctgcggcgtg aacaggctgg agccagtgcc ggactgaccg gacggcgggt
     8761 tccagttgcc ctgctgctgg tacgaatcgt tgtggccgct gccctggctc tgcgactgga
     8821 agctgccggt ctgcagcagg ccgggctggt tcgcgaccag cgtcgagagt tcctggcaga
     8881 gggcatcgac gcgcgacttc aggccgtggt tgaacatgtc gcccagcatc agcatgccgc
     8941 cggccatcca ctggcccgaa ccaccgaatt cgtggtggct gaactgcgcc atcgtgccgt
     9001 tgccgttgat gacggcgtcg agcatggcgc gtacggcatc gctgctgaag ccatgacggc
     9061 gggaaatatc gtcgacggcc tgctggccgg cgggggaaag ctgtcgcatc ggattcatct
     9121 cgcttccgga cgggcagggg ttgttcgcca cggtccggtc agatagccgt atttacggcg
     9181 gggggcgagt ctaggagggt cgcgcgatgg aggcaacgcg tttcgcacga ttgtcgcctg
     9241 taaccgacaa atcagggttt cccgcccatt cgtcgccaga tcgccggaat ttctcgtcgg
     9301 atcgctgtcc gtccgtgatc ccgctcggct acgctcgacg cagatgccgc attgccggtg
     9361 ccatcgcgcc ggcaatgccg ttgcgatcga atctgcccga aaccgaatct gcccggaggt
     9421 ggatgcgcca tgcccggacg cgtcgaattt tcctttggcc cgcagcgccg cagcgcccgg
     9481 accgatggca gcacggtctg ccggatcctg atcctcgccg atctggtcgg cgaaaccctg
     9541 cgtcccccga ttgccgaacg ccagccgatg cgcgtcagtg ccgcaacgct cgatgcgctg
     9601 atggccagac tggcaccgcg gctcgaactg gccgccggtt ccggcctgcc ggcgctgacg
     9661 atcgacttcg ccagcctcga cgacctgcat ccggatcagc tgctggcgcg cgtacccttg
     9721 tttgcagaca tcgacgccag cgcccggcgg ctggccgtgc agccgcaggc cgcaccgccg
     9781 gttccagccg gacagccggc atcgccggcg gaggacttcg ccgcgctgct cggcggcagc
     9841 gtcggcagga gcgctgccgg cagcgacatg cagcagaaag ccgaggattt cgtgcgtcgc
     9901 ctgctcgccg gcacgtcatc ggcaacgccg gtgccggacc cgcgccagac cgtgctcgat
     9961 gccgcctgcg cactggcccg cacgcaggcc ctgcgggcct tgctgcacca tccggacttc
    10021 cagcggctcg aatccgcctg gcgttcgctg gcgatgctgg tggcgggcgt cgacaacgat
    10081 gccggcgtcg aggtctgggt gctcgacatc ggtcgctgcg aactctgcgc cgatctcgcc
    10141 gccgacctgc cgatcctggc gaacctcatc ctgcagagtg ccgaacagac gggggagtgg
    10201 ggactgctgg tcagcaccga aaccttcggc gcctcggccg aagacgtgac cgtgctggca
    10261 gccttcggcg ccatcgccgg ggactgtggc gccacggtgc ttgcaggcat ggacccggcc
    10321 atcaccatcg atccggccgg tttcgcggcc gatgccattt ctgccgcgcg ctggtcagcc
    10381 gtgcgggccc tggtcggcag caaccggatc gcgctcgcgg ctccgcgcct gctgctgcgc
    10441 cgtccctatg gcgcgcgctc cgatccgctt tcctcggccg ccttcgagga agtcgatgcg
    10501 tgcccggcgc acgaggattt cctctggggc agtggtggtg tcgggcttgc cctgttgctc
    10561 gggcaggcgt gggagcagaa cgaaggctgg gaattcgacc cggaccagtg gcgggaactc
    10621 gacgacctgc cgatggcact gctcgcccgc aacggcgagc gcgaactcgt gccctgcgcc
    10681 gagcgctggt tgagcgatgt cgccgcccag accctcgatg gtctgggcgc caccgtgctg
    10741 ctcagtcgac gcgaccgcaa tcaggcctcg gtcctccgtt tccgctcact ggccggcgcc
    10801 ctgcggggac gctggaacga ctgaaatcaa aatcaatcgt gcagggatgc acggttattt
    10861 ttaatttgta tttttatacc atcatatttt caataaaact taaataaaac cggatatctg
    10921 acaactgcca gatatccgga aaacagaagc attcaggcag ccgtcggaac gcccgcgccc
    10981 gtcgccgtaa cggctcgatc cagaaacacc ttcatgcgcc gcggccgcac ccagaccggt
    11041 tcgccggtgg cgtagcctt
//
LOCUS       DAOQIE010000105        11042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2947_length_11042_cov_7.669701, whole genome shotgun sequence.
ACCESSION   DAOQIE010000105 DAOQIE010000000
VERSION     DAOQIE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11042
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2947_length_11042_cov_7.669701"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK144_08240"
     CDS             <1..293
                     /locus_tag="PK144_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNF65753.1"
                     /translation="PAPQQVDIDPILLRPVDDLELTVRSANCLKAESIYYIGDLIQRT
                     EVELLKTPNLGKKSLTEIKDVLASHGLSLGMKLENWPPPQLAGDKADDRASA"
     gene            373..747
                     /gene="rplQ"
                     /locus_tag="PK144_08245"
     CDS             373..747
                     /gene="rplQ"
                     /locus_tag="PK144_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNF65754.1"
                     /translation="MRHRKSGRKLNRTSSHRDAMFRNMTASLIAHELIKTTLPKAKEL
                     RRVAEPLITLAKSDSVAGRRLAFARLRDRDAVTKLFNELGPRYTTRPGGYLRILKCGF
                     RAGDNAPMAYVELVDRPAAAAE"
     gene            complement(842..1051)
                     /locus_tag="PK144_08250"
     CDS             complement(842..1051)
                     /locus_tag="PK144_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65755.1"
                     /translation="MPPAPALPITTDPAASTLRRLRLSDEIAFLESRLAEMGHGGDCA
                     YEHALSRAYTDRLIERRQELSRLLH"
     gene            complement(1134..1964)
                     /gene="nadC"
                     /locus_tag="PK144_08255"
     CDS             complement(1134..1964)
                     /gene="nadC"
                     /locus_tag="PK144_08255"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNF65756.1"
                     /translation="MNAADIAAQVRAALAEDIGSGDLTADLIPATNRAEATVIAREPA
                     VICGVAWFDEVFRQIDPSIRIDWRVRDGERVSADQLLCTLHGPSRALLSGERTALNFL
                     QTLSGTASLAKRYAEAVAGTGATVLDTRKTLPGLRLAQKYAVRCGGCSNHRIGLYDAI
                     LIKENHIMAAGSITAAVTQARALHPGIRIEVEVETAEELAEAVACTPDVIMLDNHDVP
                     RMREAVAFVAGRIPLEASGNVSLDTIRAYAETGVDYISVGGLTKDVRALDLSMRFRSR
                     "
     gene            complement(1999..2616)
                     /locus_tag="PK144_08260"
     CDS             complement(1999..2616)
                     /locus_tag="PK144_08260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF65757.1"
                     /translation="MLPPLTRHHWIFDLDGTLTVAVHDFPAMRRRLGIPGHVDIIDWI
                     DTRGAAEAARLHRALDAIEHEYASLARAAEGMPELLAALETAGCRLGLLTRNTREVAG
                     QVLRRLGVDRHFAEETLFGRHEAPPKPRPDGISRLLQHWQAEAGDTVMVGDHLHDLLS
                     GRAAGTATVLISPSDPPPDWRIHIDCHFGSPGQLLAGWLAARPPT"
     gene            complement(2625..3938)
                     /locus_tag="PK144_08265"
     CDS             complement(2625..3938)
                     /locus_tag="PK144_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNF65758.1"
                     /translation="MNRRHPPALLVGLLLACFAIEAPAVDLLQVYRDAKDGDPQLRAA
                     DASRQAAGENKPQAIANLLPQIGASLERDHDSAVRGLNGVSSYNSHSYGVTLTQPIFR
                     KENYVQLRRADALVGQAEADYADADQQFLLRVATRYFTVLASLDNLRFARAEKDAVAR
                     ELEQARRRFDVGLVTVVDVQEAQARHDLTVAQEITAENTLEDAREALRVVTGQEYGPL
                     DELGERMPLKMPDPARVDPWIDRALANNPTLRSAQQAADAAKETVDLQRAGHYPSLDL
                     VASHYDNDFGPRGGDLSGNRVGIQVNIPLFQGGAVTSRTRQAAYQHEAAREQVEATQR
                     DIVRQVRNAHRGQQAAISQVKALKQALVSNRSSLDATRAGLDVGTRTIVDVLDAEREL
                     YRAERDYAKARYDYVLNRLLLKQAAGDLGEADLVEINAWLQRPKS"
     gene            complement(4053..4373)
                     /locus_tag="PK144_08270"
     CDS             complement(4053..4373)
                     /locus_tag="PK144_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF65759.1"
                     /translation="MRQLDPLELITWRRGLQENPQYLDVRERWEFALCRLEDSINIPL
                     GELAERYDEIDGERPVVCICHHGIRSLHAASFLEHEGFVEVVNLAGGIDAWARLADPA
                     VPRY"
     gene            complement(4386..5036)
                     /locus_tag="PK144_08275"
     CDS             complement(4386..5036)
                     /locus_tag="PK144_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNF65760.1"
                     /translation="MNFEQARFNMIEQQIRPWEVLDQQVLEVLAKIRREAFVPEQYRQ
                     LAFSDIRIPLGHGETMMNPNVEGRLLQALSVRPEDRILEIGTGSGYVTACLATLGSSV
                     LSVDIHADFTETAQARLQAAGLHNVQLQTGDAARGWGNLRYDVIAITGALPVLDPLWR
                     DLLTVGGRLFAVVGTAPIMEAILVTRVGEQEWTSESLFETELPCLLNSARPPVFTF"
     gene            5300..6025
                     /locus_tag="PK144_08280"
     CDS             5300..6025
                     /locus_tag="PK144_08280"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNF65761.1"
                     /translation="MSEHGDWQELLASGPALDALNLRLEAMSAEQRMEWAAGRFGDAL
                     VLSSSFGAQAAVSLHMATRVLPGIPVILVDTGYLFPETYRFVEDLTERLKLNLHIYRS
                     PLSPAWQEARYGRLWEQGLEGIEHYNRINKVEPMQRALDELQAQAWIAGLRRQQAKSR
                     SGLSVALPRNGRLKVHPIIDWTDRDVGMYLRRHDLPYHPLWEQGYVSIGDVHTTTRLA
                     DGMTEEETRFFGLKRECGLHEHV"
     gene            6067..6618
                     /locus_tag="PK144_08285"
     CDS             6067..6618
                     /locus_tag="PK144_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF65762.1"
                     /translation="MRIHPTRLTASARLAAVSGWLLALAGWRCAGQPPAGSCVAIGAP
                     HTSNWDFCLMLALALHHRLRLVWLGKHTLFCGPLGWLMMWLGGVAVDRTRTNGLVETA
                     VEAFRAHEQLILVIAPEGTRRRGVRWKTGFWHIADAAGVPVVLGYIDWRNKVVGLGPA
                     MHTSGDLDADLMAIRAFYQPYER"
     gene            complement(6642..7799)
                     /gene="rnd"
                     /locus_tag="PK144_08290"
     CDS             complement(6642..7799)
                     /gene="rnd"
                     /locus_tag="PK144_08290"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNF65763.1"
                     /translation="MSAADWLYVDTAPELEALVARLQGSPWLALDTEFIRERTYYPQL
                     CLLQVATPGLIACVDPLALPDIGPLLDVLYDPATVKVLHAASQDLEIFYHLRGAVPQP
                     VFDTQIAASLLGHGEQAGYGTLVESLLSITLDKSQSRTDWAQRPLDATQLAYAADDVR
                     HLRSVYGLLLDELARLGRRDWLEQDFIDLCRSDRYAPNPPEAWRRIKGARMLRGVQLA
                     VLARLAEWREQQAMASDRPRKWIAADDLLLDLARVQPRDEAQLGKLRTLEAATARRHG
                     ATLLALVAEARAQPKESWPQLPQRRQLAAEQEALVDAMMAIVRLRGLQKGVSTAQLAA
                     RRELEQLVEGERDLPLLHGWRAGVAGREVLDLLEGRLALAVRDGQLLTFDR"
     gene            complement(7801..8283)
                     /gene="moaC"
                     /locus_tag="PK144_08295"
     CDS             complement(7801..8283)
                     /gene="moaC"
                     /locus_tag="PK144_08295"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNF65764.1"
                     /translation="MSEFTHFNAAGAAHMVDVGTKPDTHRVAIAEGRITMHAGTLAKI
                     LAGDHRKGDVLGIARIAGIMGAKRTADLIPLCHPIALTRVDIEFDTQAIPDAVLCRAT
                     AETVGPTGVEMEALTAVQTALATIYDMCKAVDRGMCIEAVRLLEKRGGRSGHWQRSAD
                     "
     gene            complement(8355..8888)
                     /gene="ppa"
                     /locus_tag="PK144_08300"
     CDS             complement(8355..8888)
                     /gene="ppa"
                     /locus_tag="PK144_08300"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNF65765.1"
                     /translation="MILDRVTAGRDLPNDFNVIIEIPANADPVKYEVDKETGALFVDR
                     FMNVAMHYPCNYGYIPNTLCGDGDPLDVLVVTPFPLVSGCVVRCRPLGLLRMSDEGGQ
                     DSKLVAVPIKKQCTLYENVEKIEDLPEVTLKQIAHFFEHYKDLDKGKWVKVDGWGSLD
                     AAQAEIADSVRLYNEAK"
     gene            9332..9886
                     /locus_tag="PK144_08305"
     CDS             9332..9886
                     /locus_tag="PK144_08305"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNF65766.1"
                     /translation="MRIVLLGAPGSGKGTQAENIVARYGITHVSTGDLLRAEVAAGTE
                     LGKKAKAIMDAGGLVSDEIVLGMIEERMNKGLSAGFLLDGFPRTLAQAEALDAMLAKL
                     EKPLDCTLFFDVDFDEITKRLLSRGRADDNEDTIRKRLAVYEEQTAPLISYYEGTGKL
                     KTVKGVGSVDEISQRIFDVLDAHR"
     gene            complement(10053..>11042)
                     /gene="gltA"
                     /locus_tag="PK144_08310"
     CDS             complement(10053..>11042)
                     /gene="gltA"
                     /locus_tag="PK144_08310"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNF65767.1"
                     /translation="KQKTDFESTIKKHTMVHEQLTKFYSGFRRDAHPMAVMTGVVGAL
                     SAFYHDAMDFSDAEHRNISFNRLVAKMPTIVAMAYKYNTGAPFMYPDNDLDYAANFMR
                     MMFGNPCEKYTPNPVLVRALDIIFTLHADHEQNASTSTVRLAGSSGANPFACIAAGIA
                     CLWGPAHGGANEACLQMLEEIGDVSRVGEYIKRAKDKNDSFKLMGFGHRVYKNFDPRA
                     KIIKGICHQVLSKYGNDPRMELAMRLEEIALKDEYFVERKLYPNVDFYSGIIYKALGI
                     PVEMFTPMFAIARTVGWISHWMEMISDPSKKIGRPRQLYIGAAKRDVPAISAR"
BASE COUNT         1824 a         3485 c         3801 g         1932 t
ORIGIN      
        1 agccggcgcc gcagcaggtc gacatcgacc cgatcctgct gcgcccggtc gacgacctcg
       61 aactgaccgt gcgttcggcc aactgcctga aggccgaaag catctactac atcggtgacc
      121 tgatccagcg caccgaggtg gagctgctga agacgccgaa tctcggcaag aagtcgctga
      181 ccgagatcaa ggacgtgctg gcctcgcacg gcctctcgct cggcatgaag ctcgaaaact
      241 ggccgccgcc gcagctcgcc ggcgacaagg ccgacgatcg ggcgagcgcc tgatcgcagc
      301 gcggggaagt cgccgcaggg ccgcttcccc gcgccttggg cgccccctga atccattcaa
      361 ggaatcacca ccatgcgtca tcgcaaatct ggtcgtaagc tgaatcgcac cagcagccat
      421 cgcgacgcga tgttccggaa catgaccgcg tcgctgatcg cccacgagct gatcaagacc
      481 acgctgccaa aggccaagga actgcgccgg gtcgccgagc cgctgatcac tctcgccaag
      541 tccgacagcg tggccggtcg ccgtctggct tttgcgcgcc tgcgtgatcg cgacgccgtg
      601 accaagctgt tcaacgaact cggtccgcgc tacaccacgc gtccgggtgg ttacctgcgc
      661 atcctgaagt gcggcttccg cgccggcgac aacgcgccga tggcctacgt cgaactggtc
      721 gatcgtccgg ctgccgccgc cgagtgatcc ggggcgttct tcatcgcttc gaaaataaaa
      781 aaccgggcat cgcccggttt tttactggcc gtttctcatg ggccgggcct ggcgcgggtt
      841 ttcagtgcag gagtcgcgac agctcctggc ggcgttcgat caggcgatcg gtgtaggcac
      901 ggctcagcgc gtgttcgtac gcgcagtcac cgccgtggcc catctctgcg agccgcgatt
      961 cgagaaaggc aatctcgtcg ctgagccgca gccgccgcag ggtcgaagcg gcggggtcgg
     1021 tggtgatcgg cagggcaggg gcagggggca tgatggcggt agtccgggat cgtggctgaa
     1081 ccccgccatc attgcgggcg gcgatgacgt ccgtatgacg cgagcggtgc cggtcagcgg
     1141 ctgcgaaagc gcatcgacag atcgagggcg cgcacgtcct tggtcagtcc gccgacggag
     1201 atgtaatcga cgccggtttc cgcataggcg cggatcgtgt ccagactgac gttgcccgag
     1261 gcctccagcg gaatgcgtcc ggcgacgaag gccaccgcct cgcgcatgcg cggcacgtca
     1321 tggttgtcga gcatgatcac gtcgggggtg caggccaccg cctcggcgag ttcctcggcc
     1381 gtctcgacct cgacctcgat cctgatgccg gggtgcaagg cacgggcctg ggtgacggcc
     1441 gccgtgatcg agccggccgc catgatgtgg ttctccttga tcaggatcgc gtcatagagc
     1501 ccgatgcggt ggttgctgca gccgccacag cgcacggcgt acttctgggc gaggcgcagg
     1561 ccgggcaagg tcttgcgggt gtcgagcacg gtggcgccgg ttccggccac ggcttcggca
     1621 taacgcttcg ccaggctcgc cgtgcccgac agggtctgca ggaagttcag cgccgtgcgt
     1681 tcaccgctca gcagcgctcg cgacgggccg tgcagcgtgc ataacagctg atcggcactc
     1741 acccgttcgc catcccggac ccgccagtcg atgcggatcg acggatcgat ctggcggaac
     1801 acctcgtcga accaggccac accgcagatc acggcaggct cgcgggcgat gaccgtggct
     1861 tcggcgcgat tcgtggccgg aatcaggtcg gcggtcagat cgccgctgcc gatgtcctcg
     1921 gcaagcgcag cgcggacctg ggcagcgatg tcggcggcat tcatgacgcg ttcgtctccg
     1981 gggggcggct actactactt atgtgggagg gcgggcagcg agccagccgg cgagcagctg
     2041 gcccggactg ccgaagtgac agtcgatgtg gatgcgccag tccggtggcg gatcgctggg
     2101 gctgatcagc acggttgcgg tgccggctgc gcgtccgctc agcaggtcat gcaggtgatc
     2161 gccgaccatg acggtatcgc cggcttcggc ctgccagtgc tgcagcagtc gggagatgcc
     2221 gtccggtcgc ggcttgggcg gcgcttcgtg ccggccgaac aatgtttctt cggcaaaatg
     2281 ccgatccaca ccgagccttc gcaacacctg cccggcgacc tcgcgggtat tgcgggtcag
     2341 cagtcccagc cggcagccgg ccgtttccag ggccgcgagc agttcgggca taccctccgc
     2401 cgcgcgtgcg aggctcgcgt attcgtgttc gatggcatcg agggcccgat gcaggcgcgc
     2461 ggcttcggct gcgccgcggg tgtcgatcca gtcgatgatg tcgacgtgcc cgggaatgcc
     2521 gagccggcgg cgcatcgccg gaaagtcatg gaccgcaacc gtcagcgtgc cgtcgagatc
     2581 gaaaatccag tggtgacgcg tcaggggcgg cagcacggct cgggtcagga tttcgggcgc
     2641 tgcagccagg cgttgatctc gaccagatcg gcttcgccga ggtcaccggc tgcctgcttc
     2701 agcagcaggc ggttcagcac gtagtcgtag cgtgccttgg catagtcgcg ttcggcccgg
     2761 tacagctcgc gctcggcatc gagcacgtcg acgatcgtgc gcgtgccgac atcgaggccg
     2821 gcgcgcgtgg catcgagcga gctgcggttc gacaccagcg cctgcttcag ggccttgacc
     2881 tgactgatcg ctgcctgctg gccacgatgg gcgttgcgga cctggcggac gatgtcacgc
     2941 tgcgtggcct cgacctgctc gcgtgccgct tcgtgctggt aggctgcctg ccgggtgcgc
     3001 gaggtcacgg caccgccctg gaacagcgga atattcacct ggattccgac gcggttgccg
     3061 gacagatcgc cgccgcgcgg gccgaaatcg ttgtcgtaat ggctggcgac caggtcgagc
     3121 gacggataat ggccggcacg ctgcaggtcg acggtttcct tggcggcatc ggccgcctgc
     3181 tgcgccgagc gcagcgtcgg gttgttggcc agggcgcggt cgatccacgg atcgacgcgg
     3241 gccggatccg gcatcttcag cggcatgcgc tcgccgagtt catcgagcgg cccgtattcc
     3301 tggccggtga cgacacgcag ggcttcgcga gcgtcttcga gcgtgttttc ggcggtgatt
     3361 tcctgcgcga ccgtcaggtc atggcgggcc tgcgcttcct gcacgtcgac gacggtgacc
     3421 agacccacgt caaagcgccg ccgggcctgt tccagttcgc gggcgacggc gtccttctcg
     3481 gcgcgtgcga agcgcaggtt gtcaagtgag gcgagcacgg tgaaatagcg cgtggcgacc
     3541 cgcagcagga actgctggtc ggcatcggca taatcggctt cggcctggcc gaccagtgca
     3601 tcggcccgcc gcagctggac gtagttttcc ttgcggaaaa tcggctgggt gagcgtgacg
     3661 ccgtagctgt gcgagttgta cgacgaaacc ccgttcaggc cgcgtacggc ggagtcgtga
     3721 tcccgctcga gcgaggcacc gatctgtggc agcaggttgg caatggcctg gggcttgttc
     3781 tcaccggcag cctgtcggct ggcgtcggca gcgcgcaact gcggatcgcc gtccttggca
     3841 tcccggtata cctgcagcag gtcgaccgcc ggggcttcga tggcgaaaca ggccagcagc
     3901 aggccgacga gcagggcagg agggtggcgg cggttcatgg cgatccatca tgcggcgagc
     3961 aagggagaca gggtggcatg acctagaggc ctcacgtcat gcaggcgtcg gggtatacgg
     4021 gcaatggcgc gatccattcg tcaatgccgg catcaatacc ggggtacggc agggtctgcc
     4081 agtctcgccc aggcatcgat gccgccggcc agattcacca cctcgacgaa accttcgtgc
     4141 tcaagaaaac tggccgcgtg caggctgcgg atgccatggt ggcagatgca gaccaccggt
     4201 cgttcgccgt cgatctcgtc ataccgctcg gcgagttcgc cgagcggtat gttgatcgaa
     4261 tcttccagcc ggcacagcgc gaactcccag cgttcgcgca cgtcgaggta ctgggggttt
     4321 tcctgcagtc cgcgccgcca ggtgatcagt tccagcggat cgagctgacg catgacggat
     4381 ctggctcaga acgtgaaaac gggcgggcga gccgaattca gcaggcaggg cagttcggtc
     4441 tcgaacaggc tctcgctcgt ccactcctgc tcgccgacgc gggtcaccag gatcgcttcc
     4501 atgatcggcg ccgtgccgac caccgcgaac aggcggccgc cgaccgtcag caggtcgcgc
     4561 cagagcgggt cgagcaccgg cagggctccg gtgatcgcga tcacgtcgta gcgcaggttg
     4621 ccccagccgc gcgcggcatc gccggtctgc agctgcacat tgtgcaggcc ggcagcctgc
     4681 agccgggcct gcgcggtttc agtgaaatca gcgtggatgt cgaccgacag cacgctggag
     4741 ccgagcgtgg cgagacaggc cgtgacgtag ccggagccgg tgccgatttc gaggatgcgg
     4801 tcttcgggac gcaccgagag ggcctgcagc agccggcctt caacgttcgg attcatcatc
     4861 gtctcgccgt ggccgagcgg aatgcggatg tccgaaaacg cgagctggcg gtactgctcg
     4921 ggtacgaagg cttcgcgccg gattttcgcg aggacttcca gcacctgctg atccagcacc
     4981 tcccaggggc ggatctgctg ttcgatcatg ttgaagcggg cctgctcgaa gttcatgtcg
     5041 ctcatcatcg aaatccctag gaagccggaa gggttcaggc gccgctgcgc cacaggaggc
     5101 gggggagtgt atcacaggcg ggagcgccac cggctcggct cagtgtggac ggttcagcgg
     5161 cgcgggtcgg cgggaagcgt gctctgctga tgccggatgg aataagcata tccgcattga
     5221 ttacttttgg ttttaaagcg gctccggtat cgtgcgcgcc atctgataac aggcagttgt
     5281 tcggctggga gagacaccga tgagcgaaca tggcgactgg caggaattgc tcgcgagtgg
     5341 tcctgcactc gatgcgctca acctgcgtct cgaagcgatg agtgccgaac agcgcatgga
     5401 atgggcggcc gggcgcttcg gcgacgcact ggtgctgtcg tccagcttcg gtgcccaggc
     5461 ggcggtcagc ctgcacatgg cgacccgggt gctgccgggg attccggtca tcctggtcga
     5521 taccggttat ctgttcccgg agacctaccg cttcgtcgag gatctgaccg aacggctgaa
     5581 gctcaacctg cacatctacc gctcgccgct gtcgccggcc tggcaggagg cgcgttacgg
     5641 ccggctgtgg gagcaggggc tcgaaggcat cgagcactac aaccgcatca acaaggtcga
     5701 gccgatgcag cgcgcactcg atgaactgca ggcccaggcc tggatcgccg ggctgcgccg
     5761 ccagcaggcg aagagccgtt cgggcctgtc ggtggcgctg ccgcgcaatg gccgcctgaa
     5821 ggtgcatccg atcatcgact ggaccgaccg tgacgtcggc atgtacctgc gccgccatga
     5881 tctgccctac cacccgctct gggagcaggg ctacgtgtca atcggcgacg tgcacacgac
     5941 cacgcgtctg gccgatggca tgaccgagga agaaacgcgc ttcttcggcc tcaagcgcga
     6001 gtgcggtctg cacgagcacg tctgaatccc tagactgacg cgctcctgaa gccggagcgc
     6061 gtcggcatgc gtatccatcc cacccgtctc accgccagcg cgcggctcgc ggccgtttcc
     6121 ggctggctgc ttgcgctcgc cggctggcgc tgcgccggtc agccgcccgc cggcagttgc
     6181 gtcgccatcg gtgcgccgca cacctcgaac tgggacttct gcctgatgct ggcgctggcg
     6241 ctgcatcacc ggctgcggct ggtctggctc ggcaagcaca cactgttctg cggcccgctc
     6301 ggctggctga tgatgtggct cggcggtgtg gccgtcgatc gcacccgcac gaatggcctg
     6361 gtcgagacgg ccgtggaggc cttccgtgcg catgagcagc tgattctggt catcgctccg
     6421 gaaggcaccc gcaggcgcgg ggtgcgctgg aagaccggct tctggcacat cgccgatgct
     6481 gccggcgtgc cggtcgtgct cggctacatc gactggcgga acaaggtggt cgggttgggg
     6541 ccggccatgc acacgagcgg cgatctcgat gccgatctga tggcgatccg ggcgttctac
     6601 cagccgtacg agcgctgagg tccggccgga agatttcgcg ctcagcgatc gaaggtgagc
     6661 aactggccgt cccggaccgc cagcgccagc cggccttcga gcagatccag cacttcccgg
     6721 ccggcgacac cggcccgcca gccgtgcagc agcggcaggt cgcgctcgcc ttcgaccagt
     6781 tgttcgagtt cgcgccgcgc ggcgagctgc gcggtgctga cgcctttctg caggccgcgc
     6841 agccgcacga tcgccatcat tgcgtccacc agggcttcct gttcggcggc gagctgccgg
     6901 cgctgcggca gctgcggcca ggattccttc ggctgcgccc gggcttcggc aaccagcgcc
     6961 agcagggtgg cgccgtgacg ccgggcggtc gcggcttcga gcgtgcgcaa cttgccgagc
     7021 tgggcctcgt cgcgcggctg cacgcgggcc agatcgagta gcaggtcgtc ggcggcgatc
     7081 catttgcgtg gccggtcgct cgccatcgcc tgctgttcgc gccattcggc gagccgggcc
     7141 agcaccgcga gctgcacgcc gcgcagcatg cgggcgccct tgatccggcg ccaggcttcg
     7201 ggcggattgg gcgcgtagcg atcgctgcgg cagagatcga tgaaatcctg ctccagccag
     7261 tcccggcgac cgagtcgggc gagttcgtcg agcagcaggc cgtagacgct gcgcaggtgg
     7321 cgcacgtcat cggccgcata ggcaagctgg gtggcgtcga gcgggcgctg cgcccagtcg
     7381 gtacggctct gcgacttgtc gagcgtgatc gacagcaggc tttcgaccag ggtgccgtag
     7441 ccggcctgct cgccgtggcc gagcaggctg gcggcgatct gcgtgtcgaa caccggctgc
     7501 ggcaccgcgc cgcgcaggtg atagaagatt tcgagatcct gcgacgccgc atgcaggacc
     7561 ttcaccgtcg ccggatcgta cagcacgtcg agcagcgggc cgatgtccgg cagcgccagc
     7621 ggatcgacac aggcgatcag tccgggtgtg gcgacctgga gcaggcagag ttgcgggtaa
     7681 taagtgcgtt cgcggatgaa ttcggtatcg agcgcaagcc acgggctgcc ttgcagccgc
     7741 gccaccaggg cttcgagttc gggggcggtg tcgacgtaca gccagtccgc agcgctcatg
     7801 tcagtccgcg ctgcgctgcc agtggccgga gcggccaccc cgtttctcca gcaggcgcac
     7861 ggcttcgatg cacatgccgc ggtcgacggc cttgcacatg tcgtagatgg tcgcgagcgc
     7921 cgtctgcacg gcggtcaggg cttccatctc gacgccggtc ggaccgacgg tttcggcggt
     7981 ggcccggcag aggacggcat ccgggatcgc ctgcgtgtcg aactcgatgt cgacccgggt
     8041 cagcgcgatc gggtggcata gcgggatcag gtcggcggtg cgcttggcgc ccatgatgcc
     8101 ggcgatgcgc gcgatgccga gcacgtcacc cttgcggtgg tcgccagcaa ggatcttcgc
     8161 gagcgtgccg gcgtgcatcg tgatgcggcc ttcggcgatc gcgacgcggt gcgtgtcggg
     8221 cttggtgccg acatcgacca tgtgcgcagc ccccgccgcg ttgaagtgcg tgaattcgct
     8281 catggcatcc ggcgggaagg caccggagcc ggcgacggcc ggccccggcg cggaacgaga
     8341 ggatcgggcg gggattactt ggcttcgttg tacagccgga cgctgtcggc gatctcggcc
     8401 tgcgctgcgt ccagcgaacc ccagccgtcg accttgaccc acttgccctt gtcgagatcc
     8461 ttgtagtgct cgaagaagtg cgcgatctgc ttcagcgtga cttccggcag atcctcgatc
     8521 ttctcgacgt tctcgtagag ggtgcactgc ttcttgatcg gcaccgcgac cagcttggag
     8581 tcctggccgc cttcgtcgga catgcgcagc aggccgagcg gacggcagcg cacgacgcag
     8641 cccgagacca gcgggaacgg cgtcacgacc agcacgtcga gcggatcacc gtcgccgcac
     8701 agcgtgttcg ggatgtagcc gtaattgcac gggtagtgca tcgccacgtt catgaagcga
     8761 tccacgaaca gcgcgccggt ttccttgtcg acttcgtact tgaccgggtc ggcgttggcc
     8821 ggaatctcga tgatgacgtt gaaatcgttc ggcaggtcac ggcctgcggt cactcggtcc
     8881 agaatcatgt gcttttcccc ctgcaggaat atccggttgg acggaagcgc gcagcctatc
     8941 acggcgacgg tgcgaagtca ggcgggccca ggcatgttgc gtcgccggcg cgcggaaatg
     9001 cggggcagac tgctgcgcac ggggtttcgg atgacaaaat aggacaggaa aggaatttca
     9061 gggggtttgc ccggcctggc ggttgcaagg gattgattgt gcaggctgac cggcaatgtc
     9121 ctccgcatgc ggcaacaagc ttgtgcagtg ccgcaagcgg gcgtgcagcc ggttccgcag
     9181 gcggttttgc gttgacaagc cggggagaaa cagaggaaag tatgttttcg aacaaatcaa
     9241 gatgttaacg gcgccggccg gtccggacgc cgattatcct tccgcggccg caagagccgc
     9301 cgacatgcaa gatcaggatg ggaataccgc tatgagaatc gtgttgttgg gggcacccgg
     9361 ctcgggcaag ggcacgcagg cggagaacat cgttgcgcgc tatggcatca cccatgtctc
     9421 gaccggcgac ctgctgcggg ccgaagtcgc ggccggcacc gaactcggca agaaggccaa
     9481 ggcgatcatg gatgccggtg ggctggtatc cgacgagatc gtgctcggca tgatcgagga
     9541 gcgcatgaac aagggcttgt cggccggctt cctgctcgac ggcttcccgc gcacgctggc
     9601 tcaggccgaa gcactcgatg cgatgctcgc gaagctcgaa aagccgctcg actgcacact
     9661 gttcttcgac gtcgacttcg acgagatcac caagcgcctg ctgtcgcgcg gccgtgccga
     9721 cgacaacgag gacaccatcc gcaagcgcct ggccgtctac gaagagcaga ccgcgccgct
     9781 gatctcctat tacgaaggca ccggcaagct gaagaccgtc aagggtgtcg gcagcgttga
     9841 cgagatttcg cagcgcatct tcgacgttct cgacgcgcac cgctgattcc gttcccggac
     9901 ccggagtccg gtgaccccgc ccgcgccctg gcgccggcgg ggtttttcat gtccgcgtgt
     9961 caggaggatc ggctgaatcc gcccggcgcg ccggcaaata aaaaaggcga tccgcgaacg
    10021 gatcgccttt tcgtacaggc ttgcgtgggc gatcagcggg cgctgatggc cggcacgtcg
    10081 cgcttggcgg cgccgatgta cagctggcgc ggacggccga tcttcttgga cggatcggag
    10141 atcatttcca tccagtgcga aatccagccg accgtgcgcg cgatcgcgaa catcggcgtg
    10201 aacatctcga ccggaatgcc gagcgccttg taaatgatgc ccgagtagaa gtcgacgttc
    10261 ggatacagct tgcgttcgac gaagtattcg tccttcagcg cgatttcctc aagacgcatc
    10321 gccagttcca tgcgcggatc attgccgtac ttggacagga cctggtggca gatgcccttg
    10381 atgatcttcg cgcgcgggtc gaagttcttg tagacgcggt gaccgaagcc catcagcttg
    10441 aacgaatcgt ttttatcctt ggcgcgcttg atgtattcgc cgacgcgcga aacgtcgccg
    10501 atctcttcca gcatctgcag gcaggcctcg ttggcaccac cgtgtgccgg gccccacagg
    10561 caggcgatac cggcagcgat acaggcgaac gggttggcac ccgacgaacc ggccagacgc
    10621 acggtcgagg tcgaagcgtt ctgctcgtgg tcggcgtgca gcgtgaagat gatgtccagg
    10681 gcgcgaacca gcaccgggtt cggcgtgtac ttctcgcacg ggttgccgaa catcatgcgc
    10741 atgaagttgg cggcgtagtc cagatcgttg tccggataca tgaacggggc gccggtgttg
    10801 tacttgtagg ccatggcgac gatcgtcggc atcttggcca cgaggcgatt gaagctgatg
    10861 ttgcggtgct cggcgtccga gaagtccatc gcgtcgtggt aaaacgccga gagggcgccg
    10921 acgacgccgg tcatcacggc catcgggtgg gcatcacggc ggaaaccgga atagaacttg
    10981 gtcagctgct catggaccat cgtgtgcttc ttgatggtcg attcgaaatc ggtcttctgc
    11041 tt
//
LOCUS       DAOQIE010000106        11009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2963_length_11009_cov_8.421946, whole genome shotgun sequence.
ACCESSION   DAOQIE010000106 DAOQIE010000000
VERSION     DAOQIE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_11009_cov_8.421946"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK144_08315"
     CDS             complement(<1..675)
                     /locus_tag="PK144_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNF65768.1"
                     /translation="MTRLLARLLRRSIPPALSHGNALDIHTDGDACFEALWADLDAAG
                     QSIWMETYILDPDAIGLETLARLRAAAARGCEVILVYDAVGSGSLGERHLEALHAAGA
                     RVHAFNPLLRNPLRRAHAHDHGQAPRPWLFRDHRKLTLIDGRIGWLGGRNIGEHYAGE
                     RLGCPGAFRDTQVRVRGPAVADLAMAFQETLDELHAAQPPLPPASPPAGTASVEVLRG
                     NGLRDRR"
     gene            841..2727
                     /gene="parE"
                     /locus_tag="PK144_08320"
     CDS             841..2727
                     /gene="parE"
                     /locus_tag="PK144_08320"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNF65769.1"
                     /translation="MTTAYDAAAIEVLSGLDPVRKRPGMYTDTARPNHLAQEVIDNSV
                     DEAMAGHAHAIDVVLHADGSVTVTDDGRGMPVDLHPEEGIPGVELILTRLHAGGKFSN
                     ANYRFSGGLHGVGVSVVNALSKRLEVVVKRGGQRWGMQFADGNKASELAALGSCPKRE
                     TGTTVRFWPDPRYFDSPKLSVSRLRHVLRAKAVLCPGLRVSFTDEEANERVEWCYSDG
                     LTDYLKEALAGVELLPDPPFTGRFEGEREAVEWAVVWLPEGGEAITESYVNLIPTAQG
                     GTHVNGLRTGLTEAVREFCEFRNLLPRGVKLAPEDVWDGTGYVLSARLAEPQFSGQTK
                     ERLSSRECAVFIAGVVKDAFSLWLNQHVEQAERIAALALAAAGKRLRAAKKVVRKRVT
                     SGPALPGKLADCAAQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEV
                     ESGEVMASQEVHDIAVAIGVDPGAEALDGLRYGKVCILADADSDGLHIATLLCALFVR
                     HFRPLVAAGHVYVAMPPLYRIDVGKEVWYALDEAEKDAQLDRLLAEKKKGKVQVTRFK
                     GLGEMNPLQLRETTMAPDTRRLVQLTLEHGDDTDPMLDMLLARKRAADRRQWLETRGN
                     LAQV"
     gene            2876..3433
                     /locus_tag="PK144_08325"
     CDS             2876..3433
                     /locus_tag="PK144_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65770.1"
                     /translation="MRVPAVIDVEASGFGRGSYPIEVGYVLPDGRRFCTLIRPQPGWQ
                     HWDAGAERIHRIPKSILQSRGREPAEVCERLDADLGGRVVYSDAWSHDLSWLHRLYDA
                     AGSTPAFRLESIRSLLGEQELAGWHPAREAAARDLCGERHRASVDALSVQHAYCRLKG
                     LSFPEPSALPGPAPMPRLVLSRLAC"
     gene            3541..5778
                     /gene="parC"
                     /locus_tag="PK144_08330"
     CDS             3541..5778
                     /gene="parC"
                     /locus_tag="PK144_08330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNF65771.1"
                     /translation="MSVPSASAGTEAVPLREFTERAYLDYSMYVILDRALPHVGDGLK
                     PVQRRIVYAMSELGLTAAAKHKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLIDGQGNWGSVDDPKSFAAMRYTEARLAPYAQTLLAELGQGTVDWQPNFDGTLEEPV
                     LLPARLPNVLLNGTTGIAVGMATDIPPHNLREIAAACVCLLDEPDAGIERLCELVPGP
                     DYPTGGEIVTPREELAKLYRSGNGSVRVRARWEHDKGSDEIVITELPFQTSGARVLEQ
                     IAAQMQAKKLPMVEDLRDESDHENPIRLVVVLRSNRVDVEPLMDHLFATTDLEKSFRA
                     NLNLIGLDGRPRVMDLRELLGEWLVYRRQTVTRRLRHRLERVEARLHLLEGLLIAFLN
                     LDEVIRIVRYEDDPKAALMARFALSVLQAEAILETKLRQLAKLEEMKIRGEQDELARE
                     RDELLATLGSEKRLTRLLRTELLADAEKYGDARRSRLVQRRAAQALDETALAPSEPVT
                     VVLSQKGWIRAGKGWELDGSSLTYKSGDAFLAQAQGRSNQLAVCLDDGGRAYTLSVHD
                     LASARGYGEPLTGRLDPPAGARFEHLLLGTPDELWLLASDHGYGYVCRYEDLLGRNRS
                     GKASVSLSAGARLLAPLRVADAAGLRIAIAGSSGHLLVFPLAELPQLAKGKGNRLIAL
                     APDKGERVAALALLPAGAGLMLVSGKRRLKVKIEDHLGERGRRGVLVGGGKVRIDALE
                     VSGAG"
     gene            5942..6736
                     /locus_tag="PK144_08335"
     CDS             5942..6736
                     /locus_tag="PK144_08335"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNF65772.1"
                     /translation="MSQTLNALDDLPLQPQLRDLSPDRPLVWLRKGWRDTLATPVASL
                     LYGGLFVLMGWLLIHHVAAGNLQLAFTLAWGFMLVGPFLATGFYELSRRLEAGEPAQL
                     WHAITALRHNPAALLLLGLASTLFLVAWVRVSAVVYAIVLGGSPDMTLAQIAHSLMSV
                     EGVVFIAFYFAIGGVLATFAFCLSVISFPMLLDRRADVITAIVASFGTAYRNRATMLR
                     WAGWIALLTGLGFVTVIGLALTLPLIGHASWHAYRDAVDYDALPPA"
     gene            7079..8494
                     /locus_tag="PK144_08340"
     CDS             7079..8494
                     /locus_tag="PK144_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF65773.1"
                     /translation="MSDILDRDGKKLSRRDFLKTTGLIVGGATMAGATPAWLRSAAWA
                     AGSEGIEKTDVTFGIIPLTDCAPIVVAYEKGYFKKYGLNVTVSKEASWANIRDKVAIG
                     ELDGAHMLAGMPIATTLGIGSLKQPTITAFSMDLNGNAITVSNELYDRMMAADPEAMK
                     ANPISARALKKVIDADKAAGKEPMTFAMVFPVSTHNYEIRYWMASAGINPDNDVRLIV
                     IPPPQMVANLKAGNIVGYCVGEPWNERAVEEGIGRTLITNYEIWNNKLEKVFGVNQAW
                     AEKNPNTHKAAVMALLEAAQWMDKPENRMEVVKLIAQKSYVNAPENIVKMSMTGTFSY
                     VKGSDPRPLPDFNVFYRYAATYPWASQAAWYITQMYRWGQIEEPMNILKTARSVYRAD
                     LYRDAAKALGLPVPTTDVKTEGSHAKGWTLNEASSPIAMGPDLFFDGGKFDPLKPADY
                     LKTFPVHSMKVDLAKLAAANG"
     gene            8699..9520
                     /gene="ntrB"
                     /locus_tag="PK144_08345"
     CDS             8699..9520
                     /gene="ntrB"
                     /locus_tag="PK144_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNF65774.1"
                     /translation="MSTASTASASASPSRIRLKWLANVALPLLTFGVVLAVWSFISLK
                     ITSDIPAPTVAWQRAIELFSDPFYQKGPNDMGIGWQVLYSLGRVLGGFALAVLVGIPV
                     GFMMGASETVNRAVYPLVQVLRPVSPLAWLPIGLLLFKAVDPSALFVIFITSIWPVIV
                     NTAAGVKAIPQDYMNVARVLQLGKLEITRRILIPGALPHIITGMRLSLGVAWMVIVAA
                     EMLTGGIGIGFFVWDEWNNLNVASIIVAIGVIGVVGIALEAAMNLVQNRLDYTRR"
     gene            9546..10340
                     /locus_tag="PK144_08350"
     CDS             9546..10340
                     /locus_tag="PK144_08350"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65775.1"
                     /translation="MNAYLNIDNVDMTFHTQSGTFHALRNINLKIAKGEFVSLIGHSG
                     CGKSTVLNIVAGLLNATRGGVLLENREVNRPGPDRAVVFQNHSLLPWLSVYDNVRIAV
                     DKVFGKTTSSAERDEMTRLNLKRVHMDHAIHKRPGEISGGMKQRVGIARALAMQPKVL
                     LMDEPFGALDALTRAHLQETVMELQVELGNTVIMITHDVDEAVLLSDRIVMMTNGPAA
                     TIGEVLKVDLPRPRNRLELADDVSYNHYRAEVLKFLYERQKRPDAA"
BASE COUNT         1770 a         3791 c         3714 g         1734 t
ORIGIN      
        1 gcgccggtcg cgcaggccgt tgccgcgcag cacctcgacg ctggcagtgc cggctggagg
       61 cgatgccggc ggcagcggtg gctgtgcggc gtgcagctca tcgagcgtct cctggaaggc
      121 catggccaga tcggcgaccg ccgggccgcg cacgcgcacc tgcgtgtcgc ggaaggcccc
      181 cgggcagccg aggcgctcgc cggcatagtg ctcgccgatg ttgcgcccgc cgagccagcc
      241 gatgcgcccg tcgatcagcg tgagcttgcg gtgatcgcgg aacagccacg ggcgcggtgc
      301 ctgcccgtgg tcgtgggcat gggcgcggcg cagcggattg cgcagcagcg ggttgaacgc
      361 atgcacccgg gcaccggccg cgtgcagggc ttcgaggtgg cgctcgccga ggctgcccga
      421 accgaccgcg tcgtagacca ggatcacctc gcagccgcgc gcggccgccg cccgcaggcg
      481 ggccagggtt tcgagaccga tggcatcggg atcgaggatg taggtttcca tccagatcga
      541 ctgcccggcg gcatccagat ccgcccacag cgcctcgaag caggcatctc cgtcggtgtg
      601 gatgtcgagc gcgttgccat ggctcagggc cggcggaatc gagcggcgca gcaggcgggc
      661 gagcaatcgg gtcatcgttc gcggatcctc gatggctctg gtcggacagg aaaacatgaa
      721 cgtaatgcaa gggatcgtgc gtcatcccgg cagcaggtcg gttacactga tgctgcaact
      781 gcacgtaaca gctgcagcgc aacaacgcac gtgcgttttt ccgacgactt cacgagaccc
      841 atgaccaccg cctacgacgc cgccgcgatc gaagtgctga gcgggctgga cccggtgcgc
      901 aagcgcccgg gcatgtacac cgacaccgca cgtccgaatc atctcgcgca ggaagtcatc
      961 gacaacagcg tcgacgaagc gatggccggc cacgcgcacg ccatcgatgt cgtgctgcac
     1021 gccgatggct cggtgacggt caccgacgac ggccgcggca tgccggtgga ccttcatccc
     1081 gaggaaggca ttcccggcgt cgagctgatc ctgacgcggc tgcacgccgg cggaaagttc
     1141 tcgaacgcca actaccgctt ctcgggcggt ctgcacggcg tcggcgtgtc ggtcgtcaac
     1201 gcgctgtcga agcggctcga agtcgtcgtg aagcgcggcg gccagcgctg gggcatgcag
     1261 ttcgccgacg gcaacaaggc gagcgagctc gccgcgctcg gcagctgccc gaagcgcgag
     1321 accggcacga cggtgcgctt ctggcccgac ccgcgctact tcgactcccc gaagctgtcg
     1381 gtgtcgcggc tgcggcacgt gctgcgcgcc aaggccgtgc tgtgtccggg cctgcgcgtg
     1441 agcttcaccg acgaggaagc gaacgagcgc gtcgagtggt gctacagcga cggcctcacc
     1501 gattacctga aggaagcgct cgccggcgtc gagctgctgc ccgatccgcc gttcaccggc
     1561 cgcttcgagg gcgagcgcga ggccgtcgag tgggcggtcg tctggctgcc cgagggcggc
     1621 gaggcgatca ccgagagcta cgtcaacctg atcccgaccg cgcagggcgg cacgcacgtc
     1681 aacggcctgc gcaccggcct gaccgaagcg gtccgcgagt tctgcgaatt ccgcaacctg
     1741 ctgccgcgcg gcgtgaagct cgcgcccgag gacgtctggg acggcacggg ctacgtgctg
     1801 tcggcgcggc tggccgagcc gcagttctcg ggccagacca aggaacgcct cagttcacgc
     1861 gaatgcgcgg tgttcattgc cggggtggtc aaggatgcct tcagcctgtg gctgaaccag
     1921 cacgtcgagc aggccgagcg catcgcggca ctggcgctgg cggcggccgg caagcgtctg
     1981 cgcgccgcta aaaaggtcgt gcgcaagcgc gtgaccagcg gcccggcgct gccgggcaag
     2041 ctcgccgact gtgcagcgca ggacctcgcc cgtaccgaac tgttcctggt cgagggcgat
     2101 tcggccggcg gctcggccaa gcaggcgcgt gaccgcgagt tccaggcggt gatgccgctg
     2161 cgcggcaaga tcctcaacac ctgggaggtc gagtccggcg aggtcatggc ctcgcaggaa
     2221 gtccacgaca tcgccgtcgc gatcggtgtc gatccggggg ccgaagccct cgacggcctg
     2281 cgctacggca aggtctgcat cctcgccgat gccgattccg acggcctgca catcgcgacg
     2341 ctgctctgtg cgctgttcgt gcgccacttc cggccgctgg tggcggccgg tcatgtgtac
     2401 gtggcgatgc cgccgctgta ccgcatcgat gtcggcaagg aggtctggta tgcgctcgac
     2461 gaggccgaga aggacgccca gctcgatcgc ctgctggccg agaagaagaa gggcaaggtg
     2521 caggtgacgc gcttcaaggg cctcggcgag atgaacccgc tgcagctgcg cgagacgacg
     2581 atggcgcccg acacgcgccg gctcgtgcag ctgacgctcg aacacggcga cgacaccgac
     2641 ccgatgctcg acatgctgct cgccaggaag cgcgccgccg accggcgcca gtggctggaa
     2701 acgcgcggca acctcgcgca ggtctgagcg gccaggcctg aacaggcagg gcagccgcgg
     2761 atggattccg ggttcaaaag gctacacgcg caccgtcaca gcagatgacg ggcatcaaac
     2821 cagaaggttg gagaggggaa cggtttcttt caatccggct ttgtgagggc ggaccatgcg
     2881 cgtgcccgcg gtgattgatg tcgaggcttc cggtttcggc cggggcagtt accccatcga
     2941 ggtcggctac gtgctgccgg atggccggcg tttctgcacg ctgatccgcc cgcagcccgg
     3001 atggcagcac tgggatgccg gcgccgagcg catccaccgc attccgaaaa gcatcctgca
     3061 gagccgcggc cgggagcctg ccgaggtctg cgagcggctc gatgccgatc tcggcggacg
     3121 cgtcgtctac tcggatgcct ggagtcacga cctgagctgg ctgcaccgcc tctatgacgc
     3181 cgccggcagc accccggcgt tccggctcga atcgatccgt tcgctgctcg gcgagcagga
     3241 actcgctggc tggcatccgg cgcgcgaggc tgccgcgcgc gacctgtgcg gcgagcgcca
     3301 ccgtgccagc gtcgatgcgc tgagcgtcca gcacgcctac tgccggctca agggcctgag
     3361 ctttccggag ccctcggcgc tgccggggcc ggcgccgatg ccgcggctgg tgctgtcgcg
     3421 cctggcctgc tgaacccccg cgcgcgagcc cgccggtttc gcctgcgggc tgcctgacgg
     3481 cggccggcgg ctgcgcttac actgcgcggc ccctccagca atccccgtgc ccgcgccccc
     3541 atgagtgtcc cctctgcctc cgccggcacc gaagccgtgc cgctgcgcga attcaccgaa
     3601 agggcgtacc tcgactattc gatgtacgtg atcctcgacc gcgcgctgcc gcacgtcggc
     3661 gatggcctga agcccgtgca gcggcgcatc gtctacgcga tgtccgaact tggcctgacc
     3721 gctgccgcca agcacaagaa gtcggcgcgc acggtcggcg atgtgctcgg caagttccat
     3781 ccgcacggcg attcggcctg ttacgaggcc atggtgctga tggcccagcc cttcagctac
     3841 cgctacccgc tgatcgacgg ccagggcaac tggggttcgg tcgacgatcc gaaatccttc
     3901 gcggcgatgc gctacaccga ggcgcggctc gcgccctatg cgcagacgct gctcgccgaa
     3961 ctcggccagg gcacggtcga ctggcagccg aacttcgacg gcacgctgga ggagcccgtg
     4021 ctgctgccgg cgcggctgcc gaacgtgctg ctcaacggca cgaccggcat cgccgtcggc
     4081 atggccaccg acattccgcc gcacaacctg cgcgagatcg ccgccgcctg cgtctgcctg
     4141 ctcgatgagc cggatgccgg catcgagcgg ctgtgcgaac tcgtgcccgg acccgattac
     4201 ccgaccggcg gcgagatcgt cacgccgcgc gaggaactcg cgaagctcta ccgcagcggc
     4261 aacggctcgg tgcgcgtgcg ggcgcgctgg gagcacgaca agggcagtga cgagatcgtg
     4321 atcacggagc tgccgttcca gacctcgggc gcgcgcgtgc tggagcagat cgccgcgcag
     4381 atgcaggcga agaagctgcc gatggtcgag gacctgcgcg acgagtccga ccacgagaac
     4441 ccgatccggc tggtggtcgt gctgcgctcg aaccgcgtcg atgtcgagcc gctgatggat
     4501 cacctgttcg cgaccaccga cctcgaaaaa tccttccgcg ccaacctgaa cctgatcggg
     4561 ctcgatggcc ggccgcgggt catggacctg cgcgagctgc tcggcgaatg gctggtctac
     4621 cggcgccaga cggtcacgcg ccggctgcgt caccggctgg agcgcgtcga ggcgcggctg
     4681 cacctgctcg aaggcctgct gatcgcgttc ctgaacctcg acgaggtgat ccgcatcgtc
     4741 cgctacgagg acgatccgaa ggcggcgctg atggcgcgct tcgcgctgtc ggtgctgcag
     4801 gccgaggcca tcctggagac gaagctccgc cagctcgcga agctcgaaga aatgaagatc
     4861 cgcggcgagc aggacgaact cgcccgcgaa cgcgacgaac tgctggccac gctcggttcc
     4921 gaaaagcggc tgacgcggct gctgcgcacg gaactgctcg ccgatgccga aaaatacggc
     4981 gacgcccgcc gctcgcggct ggtgcagcgg cgcgccgcgc aggcgctcga tgaaaccgcg
     5041 ctggcgccgt ccgagccggt caccgtcgtg ctgtcgcaga agggctggat ccgcgccggc
     5101 aagggctggg agctcgacgg cagcagcctg acctacaagt ccggggatgc cttcctcgcg
     5161 caggcgcagg ggcgcagcaa ccagcttgcc gtctgcctcg acgacggcgg acgtgcctac
     5221 acgctgagcg tgcatgatct cgcctcggcg cgcggctacg gcgagccgct gaccggccgc
     5281 ctcgatccgc cggccggcgc gcgcttcgag cacctgctgc tcggcacgcc ggacgaactc
     5341 tggctgctcg cctccgacca cggctacggc tacgtctgcc gctacgagga cctgctcggg
     5401 cgcaaccgct ccggcaaggc ctcggtgagc ctctccgccg gcgcccggct gctggcgccg
     5461 ctgcgcgtcg cggacgcagc cggcctgcgc atcgccatcg ccggcagcag cggtcacctg
     5521 ctggtctttc cgctcgccga actgccgcag ctcgccaagg gcaagggcaa ccggctgatc
     5581 gcgctggcgc cggacaaggg cgagcgggtc gcggcgctgg cgctgctgcc ggccggagcc
     5641 gggctgatgc tggtcagcgg caagcgccgg ctgaaggtga agatcgagga ccatctcggc
     5701 gagcgcggcc gccgcggcgt gctggtcggc ggcggcaagg tgcgcatcga cgctctggag
     5761 gtcagcggcg ccggctgaag gcgggccagc ggcgccggcg gacccggggt gcgatgacaa
     5821 tttctccacg cacacggcgg cagtgatggc cgtgctgcgg ggctcgacta cggtagcggg
     5881 cagtgccgca cgcgatggca cccccgctga tccggaccca ggagagagag gttcacgcat
     5941 catgtcgcag accctgaacg ccctagacga tctgccgctg cagccgcagc ttcgtgatct
     6001 cagccccgac cggccgctgg tctggctgcg caagggctgg cgcgacaccc tggccacgcc
     6061 ggtggcgagc ctgctctacg gcgggctgtt cgtgctgatg ggctggctgc tgatccacca
     6121 cgtcgcggcc ggcaacctgc agctcgcctt cacgctcgcc tggggcttca tgctggtcgg
     6181 gcccttcctc gccaccggct tctacgaact gtcgcgccgc ctcgaagccg gcgaaccggc
     6241 gcagctctgg cacgcgatca ccgcgctgcg gcacaacccg gcggcgctgc tgctgctcgg
     6301 gctggcgagc acgctgtttc tcgtcgcctg ggtgcgcgtc tcggccgtcg tctacgcgat
     6361 cgtgctcggc ggctcgccgg acatgacgct cgcgcagatc gcgcattcgc tgatgagcgt
     6421 cgagggcgtg gtgttcatcg ccttctattt cgcgatcggc ggcgtgctcg ccaccttcgc
     6481 gttctgcctg agcgtgatct ccttcccgat gctgctcgac cggcgcgccg acgtgatcac
     6541 ggccatcgtc gcgagcttcg gcaccgccta ccgcaaccgc gcgacgatgc tgcgctgggc
     6601 cggctggatc gcgctgctga ccgggctcgg cttcgtgacc gtgatcggcc tggcgctgac
     6661 gctgccgctg atcggccatg ccagctggca tgcctaccgc gatgcagtcg attacgacgc
     6721 cttgccgcct gcctgatcct ggcggggtag gccggcatac ctctctggtg cattacgaag
     6781 cggcaagaat cggcgtggct tgcaccgatg tggagcatgt gcggcggcgg ggtatcgggt
     6841 tcgtatgatc aagatcatga aatgattgaa aaatcatgat tggcatgagt ctggcattta
     6901 tctgatccaa gctttcagac ctgccaaagg tggtagggat cccctgaaag ccggcgacca
     6961 acggcggtcg ccaaagcgga caacggcgtc cgatacccca gcgcgcttct ccctgctgcg
     7021 ctcgggctat cgggcgcttt tttttggctt tcattttgag tgatgcagga gttcggacgt
     7081 gagcgacatt ctcgatcggg atggcaagaa gctgagccga cgcgattttc tgaagaccac
     7141 ggggctgatc gtgggaggtg cgacgatggc aggtgccact ccggcttggc tgcgcagtgc
     7201 cgcctgggcg gccggttccg aaggcatcga gaagaccgat gtgaccttcg gcatcattcc
     7261 gctgaccgac tgcgcgccga tcgtcgttgc ctacgagaag ggctacttca agaagtacgg
     7321 cctgaacgtc accgtctcga aggaagcctc ctgggcgaac atccgcgaca aggtcgccat
     7381 cggcgaactc gacggtgcgc acatgctcgc cggcatgccg atcgcgacga cgctcggcat
     7441 cggctcgctg aagcagccga cgatcaccgc gttcagcatg gatctcaacg gcaacgcgat
     7501 cacggtctcg aacgagctct acgaccgcat gatggcggcc gacccggagg cgatgaaggc
     7561 gaacccgatt tcggcccggg cgctgaagaa ggtcatcgat gccgacaagg cggccggcaa
     7621 ggagccgatg accttcgcga tggtgttccc ggtctcgacg cacaactacg agatccgcta
     7681 ctggatggcc tcggccggca tcaacccgga caacgacgtg cgcctgatcg tcatcccgcc
     7741 gccgcagatg gtcgccaacc tcaaggccgg caacatcgtc ggctactgcg tcggcgagcc
     7801 gtggaacgag cgcgcggtcg aggagggcat cggccgcacg ctgatcacca actacgagat
     7861 ctggaacaac aagctcgaaa aggtgttcgg cgtcaaccag gcctgggccg agaagaaccc
     7921 gaacacccac aaggccgcgg tcatggcgct gctcgaagcc gcgcagtgga tggacaagcc
     7981 cgagaaccgc atggaagtgg tgaagctgat cgcgcagaag tcctacgtca acgcgcccga
     8041 gaacatcgtc aagatgtcga tgaccggcac gttctcgtac gtcaagggct ccgatccgcg
     8101 gccgctgccg gacttcaacg tcttctaccg ctacgccgcg acctatccgt gggcctcgca
     8161 ggccgcctgg tacatcacgc agatgtaccg ctggggccag atcgaggagc cgatgaacat
     8221 cctcaagacc gcgcgcagcg tctaccgcgc cgatctgtac cgcgatgcgg ccaaggcgct
     8281 cggcctgccg gtgccgacca ccgacgtgaa gaccgaaggc agccacgcca agggctggac
     8341 gctcaacgag gcatcgagcc cgatcgcgat gggtccggac ctgttcttcg acggcggcaa
     8401 gttcgatccg ctgaagccgg ccgactacct gaagaccttc ccggtgcaca gcatgaaggt
     8461 cgatctcgcg aagctcgccg ctgccaacgg ctgacgttcc cgccctttcc tgaccatccc
     8521 ccgacccggg cggcgatccc gcccggtctg acggagcctc cgcgccatga ctgcgatcaa
     8581 gaaactgcag ctcgtccacc cggccccggc cgaatccgcc gcaacgccgg ttcagtccgg
     8641 gaatgacgcg gcaggctccc cggcccccac ggccggcgcc gcgaccgaga accgtccgat
     8701 gagcaccgcc agtaccgctt ccgcctccgc ttccccttcg cgcatccggc tcaagtggct
     8761 cgccaacgtc gcgctgccgc tgctgacctt cggggtcgtg ctggccgtgt ggtccttcat
     8821 cagcctgaag atcaccagcg acatcccggc gccgacggtg gcctggcagc gtgccatcga
     8881 gctgttcagc gacccgttct accagaaggg tccgaacgac atgggcatcg gctggcaggt
     8941 gctgtactcg ctcgggcgcg tgctcggcgg cttcgcgctg gcggtgctgg tgggcattcc
     9001 ggtcggcttc atgatgggcg cgagcgagac cgtcaaccgc gccgtgtacc cgctggtgca
     9061 ggtgctgcgc ccggtcagtc cgctcgcctg gctgccgatc ggcctgctgc tgttcaaggc
     9121 cgtcgatccg tcggcgctgt tcgtgatctt catcaccagc atctggccgg tgatcgtcaa
     9181 caccgcggcc ggcgtcaagg ccatcccgca ggactacatg aacgtcgcgc gcgtgctgca
     9241 gctcggcaag ctggagatca cccgccgcat cctgatcccc ggtgcgctgc cgcacatcat
     9301 caccggcatg cgcctgtcgc tcggcgtggc ctggatggtc atcgtcgctg ccgagatgct
     9361 gaccggtggc atcggcatcg gcttcttcgt ctgggatgag tggaacaacc tgaatgtcgc
     9421 ctcgatcatc gtcgccatcg gcgtgatcgg tgtggtcggc atcgcgctcg aagccgccat
     9481 gaacctcgtc cagaaccgcc tcgactacac ccgccgctga tcggttgcgt cccggagccc
     9541 atgccatgaa cgcctatctg aacatcgaca acgtcgacat gaccttccac acgcagagcg
     9601 gcaccttcca cgcgctgcgc aacatcaacc tgaagatcgc caagggcgag ttcgtgtcgc
     9661 tgatcggtca ctcgggctgc ggcaagtcga cggtgctgaa catcgtcgcc ggcctgctca
     9721 acgccacccg cggcggcgtg ctgctcgaaa accgcgaggt caaccgcccg gggccggacc
     9781 gcgccgtggt gttccagaac cactcgctgc tgccgtggct gagcgtctac gacaacgtgc
     9841 gcatcgccgt cgacaaggtg ttcggcaaga ccacctcgtc ggccgagcgc gacgagatga
     9901 cgcgcctgaa cctcaagcgc gtgcacatgg accacgccat ccacaagcgc ccgggcgaga
     9961 tctccggcgg catgaagcag cgcgtcggca tcgcccgcgc gctggcgatg cagccgaagg
    10021 tgctgctgat ggacgagccc ttcggtgcac tcgacgcgct gacccgcgcg cacctgcagg
    10081 agaccgtcat ggagctgcag gtcgagctcg gcaacaccgt gatcatgatc acgcacgatg
    10141 tcgacgaggc cgtgctgctg tccgaccgca tcgtcatgat gaccaacggt ccggccgcga
    10201 ccatcggcga ggtgctgaag gtcgacctgc cgcgtccgcg caaccgcctc gaactggccg
    10261 atgacgtctc gtacaaccac tatcgcgccg aagtgctgaa gttcctgtat gagcgccaga
    10321 agcggcccga cgccgcctga tcgcgccgcg ttcccgccgc cgttcccccc gcagttccca
    10381 tccgtttcgg tactgaccgg gcgccccctt gccggggcgc ctccggcccc tgcctgtccg
    10441 gagtccgccc atgtccagtt gcggtcgcac cgctgtccct gcgcgctgcc ggtggcaggc
    10501 gctgcgcggc gcgtatgctc cgggggacag acgttcttca agtagttttt ttataccatc
    10561 atatttttgt ttcagcttca gcacgcctcg tttcggaccc tcctcaaata caagccccca
    10621 ggtcacggag tccgtccgca ggccctccct gcgcaggcct ctttcacgaa ccacaggctc
    10681 tggaagcctg gggcgtcagg cgtgcacgga tacccggcgc gaagcggcac aagcgtcgct
    10741 gcccggacgc aggcatcgaa tccgcagcct gtgcgcgcgg ccggatggct gcggagcgca
    10801 tttccgggcc cgtggcgctg tatctgccgt gcgctcgatg gcgatgctgg tggtcccgcg
    10861 agagcagggc ttgcagatgc atgggaatgc cgcccgcccg gaagcggtcc ccgcagacgg
    10921 gttcgcgggc ttgcgcaggc cggcagggcc tgcgtctgcg gctcgtgccg tcctgccgcg
    10981 cggggcaggg cgggatgccc ggcgagccg
//
LOCUS       DAOQIE010000107        10968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_2975_length_10968_cov_7.259782, whole genome shotgun sequence.
ACCESSION   DAOQIE010000107 DAOQIE010000000
VERSION     DAOQIE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2975_length_10968_cov_7.259782"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK144_08355"
     CDS             <1..482
                     /locus_tag="PK144_08355"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNF65776.1"
                     /translation="PARLADFFAAWRTAPAGFWVLAGLAVFLSMIWFTDAAIQYGLFF
                     GSGRWSPADGFDARLGGYAAFAGLTGGGLALAAWLVNGFAMPLTCRRGLALTPAVAAG
                     LRALRAHPGVLALWAGVLGGGSLATFALCYPALVVTVPVLAYGSLAACEEIFPGSGT"
     gene            complement(431..1654)
                     /locus_tag="PK144_08360"
     CDS             complement(431..1654)
                     /locus_tag="PK144_08360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF65777.1"
                     /translation="MMFDVKTLMFFMALSALLMAGVITLADQGRAPQRSLSGLQLWTL
                     SLLVQAAGWFVLVASNSLIPAGFAGPLALGCVSLCWGLRLQALDRCAGRDGTLWRVPA
                     LVGVSAGISLLSPASEWLVGLHSLLYGLLACACARQVRRLPDHGHVTAQSIMALALLL
                     LGGVLIARSAELLLARALWRQVEIWRTVLEQASFLSGYASTIIVTIGLVLLARAGHEE
                     HLQRLAGTDALTGLLNRRSFLSRAGQALRPQTPAAVLMLDLDHFKIINDSFGHPVGDA
                     VLRTVSATLASGLRREDLLARFGGEEFAALLPQTGARTALTIGERLRTAVAAQHAGAE
                     LGGRRCTISIGIAAGSGDLPIDVLITEADRALYTAKREGRNRCVLVEVPALVDGESPQ
                     VPEPGKISSQAARLP"
     gene            complement(1872..3212)
                     /locus_tag="PK144_08365"
     CDS             complement(1872..3212)
                     /locus_tag="PK144_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF65778.1"
                     /translation="MKFPYRHALSLLTGAIAGAGLAWVGAVSAADPAAPPALPFEELR
                     AFTEILERVRHDYVEPVDDRKLLESAVRGMLAGLDPHSSYLDKEGYREMQVGTTGEFG
                     GLGIEVGQEDGFVKVISPIDDTPAARAGIRAGDLIVRIDDTPTKGLGLADSVKLMRGS
                     VGSSVKLTVVRAGEDKPLEITLTRDVITVRSVKSRALEPGYGYVRITQFQSRTAGYLR
                     EQLATLKQAGGGRLKGLVLDLRNNPGGVLSGAVEVADAFLDSGLIVYTEGRESGSRQN
                     FQAGAGDLIDGVPLVVLVNGGSASASEIVAGALQDQRRAVIMGTRTFGKGSVQTIMPL
                     ADGTAVKLTTARYYTPSGRSIQAQGIEPDIRVDAVRVTGVEAADTVKEADLAGHLNNP
                     GQRGNETGGAGEADPAQAAPAGAGRSTELPLVQSDYALAEALNLLKGLALLGRK"
     gene            complement(3495..5036)
                     /gene="gpmI"
                     /locus_tag="PK144_08370"
     CDS             complement(3495..5036)
                     /gene="gpmI"
                     /locus_tag="PK144_08370"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNF65779.1"
                     /translation="MPATRRPLVLAILDGWGYSEDPAHNAILGAHTPNWDRLWATHPH
                     TLIDTSGLSVGLPAGQMGNSEVGHMNLGAGRVVYQELTRIGLAIEHGGFFENPVLCKA
                     VDAAVAGGGAVHVMGLLSPGGVHSHEEHLQAMVRLAAGRGAQRVYVHAFLDGRDTPPR
                     SAADSLAAMDAVCAATGVARIATLVGRYYAMDRDNRWERVQAAYELIVDARGAHTAAD
                     AAAGLAAAYGRGENDEFVAPTVIGAPVTVADGDAVVFMNFRADRARELTRAFVEPDFS
                     GFARARAPALAGFVCLTQYQKSIPAPVAYAPEDLSNVLGDWLAQHGRTQLRIAETEKY
                     AHVTFFFSGGREPPYPGEERILIPSPKVATYDLQPQMSAPEVTDRLVEAIRARAFDLI
                     VVNYANPDMVGHTGLYAAAVQAVEAIDAALGRLDAALAEVGGEMLVTADHGNVESMWD
                     ADSAQANTAHTTNVVPFLYVSHAGRRGTPAGTGALCDVAPTVLKLLNLPQPPEMTGRP
                     LLALD"
     gene            5223..5648
                     /locus_tag="PK144_08375"
     CDS             5223..5648
                     /locus_tag="PK144_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF65780.1"
                     /translation="MQTFLDFAARNWLLFAAFLAILVALLVTEFLRLSRGVKAIGVQD
                     ALRRLNDETAVLVDVRDPGEFRNGHISGARHLPAGEFDRRIGELDKYRDRELIVCCQH
                     GNRSAPLAAKLKKAGFTSVMNLAGGVAAWTGAGLPLVRK"
     gene            5645..5929
                     /gene="grxC"
                     /locus_tag="PK144_08380"
     CDS             5645..5929
                     /gene="grxC"
                     /locus_tag="PK144_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNF65781.1"
                     /translation="MNAPARRPEIIVYASRWCPYCRAAFALLDRKGVAYEVLDVDEQP
                     ALWNEIDARTGRDTVPQTFIDGHHVGGYDDMAALDRAGGLDPLLFPKAPG"
     gene            6076..6567
                     /gene="secB"
                     /locus_tag="PK144_08385"
     CDS             6076..6567
                     /gene="secB"
                     /locus_tag="PK144_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNF65782.1"
                     /translation="MSEQNSAPQQPQQHFEIQKLYTKDISFETPNSPMIFTEQWTGES
                     QLQLHNESAQLADNIHEVVLTVTVTTKQGDKTAYLCEVRQAGIFTIAGFAPDQLAAML
                     GAYCPNVLFPYAREAVSNLIQRGGFPPLQLAPVNFDALYMQHLQAKQAQQMQAGQPSS
                     GTA"
     gene            6611..7606
                     /locus_tag="PK144_08390"
     CDS             6611..7606
                     /locus_tag="PK144_08390"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNF65783.1"
                     /translation="MATLAVLGAGSWGTALALLLARNGHAVRLWGHDPAQVARLATER
                     ENAAFLPGVTLPGRITPGADLAAACAGAAMVLVVVPSHAFVATLDALAALAQPPAALA
                     WATKGLEPRTGRFLHEVARERLGSRPLAVVSGPNFAREVARGLPTATTVASLDAGWAA
                     TVAGFLHGRDFRAYTVTDVSGVELGGAVKNVLAIACGVADGLGYGANARAALITRGLA
                     EMVRLGLACGGRRETFMGLAGVGDLVLTCTDDQSRNRRFGLALGRGAGVGEALAGIGQ
                     VVEGAAAVAEVLKLARSHGVEMPISEQVEAVVHGGRPPAAAVEALLAREPRDDGV"
     gene            7616..8059
                     /locus_tag="PK144_08395"
     CDS             7616..8059
                     /locus_tag="PK144_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNF65784.1"
                     /translation="MYGWLLLLHILGASVWTGGHLVLSLVILPGVLRERSAAKLLAFE
                     SGFEKIGMPALVVQVVTGLLLAWRQLPQLTAWFDPANPLAHAILAKLGLLLATFLLAL
                     DAKLRVLPHFDDSRLTDMAWHIVPVTVLSVLFVAAGVSFRTGWLY"
     gene            complement(8126..9010)
                     /locus_tag="PK144_08400"
     CDS             complement(8126..9010)
                     /locus_tag="PK144_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNF65785.1"
                     /translation="MRPDTLRKHTHSEPSARSHALPRNSRKARHLALAGLGGLLAAMV
                     LLAPKPVRAESGKLSIDRRCVLPAQMVRLDGALERTRARIDRREPLTIVAIGSSSTEG
                     YGASSPANTYPAQLGSLLRTRYVGEPIEVINKGRGGEVATQTLARFGKDVYPNDPTLV
                     IWQAGANDVVRNVPLEQFAATLKEGLAALRSAGIDVILMPPQYAPQTAAAKQIEAYVE
                     LIEQLGDEFDVPVVQRYELMQQLAAKDAPLLAAMLTADGLHQNDLGYHCLAAQVAMGI
                     DTLDAGSTGGTLATTLAP"
     gene            complement(9051..9851)
                     /locus_tag="PK144_08405"
     CDS             complement(9051..9851)
                     /locus_tag="PK144_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65786.1"
                     /translation="MTPPRCRRPVLFLLAALLGAGVHAEVVPPVPSADEDDDSPVAPE
                     VSECGDPSLIRGTPFPLSHVAAARLENRDVTILVLGTASTAGRAGPTSPTLSYPVRME
                     HWLEARWGDRRVHVVNLARPGQTAAQQIEKLPEALATYHPELLVWQTGTVDAIRGVDI
                     NEFGDAIEHGLQYAENAAADTLLIDAQYGSEAFVLYDAGPYLGYLRQIAMGRRAALFP
                     RFEIMRRWVEQGLLDLDPADAESQRRVADRIHDCLGRYLADMIDRASR"
     gene            complement(9848..>10968)
                     /locus_tag="PK144_08410"
     CDS             complement(9848..>10968)
                     /locus_tag="PK144_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HNF65787.1"
                     /translation="AGRDARLDFFRGLALIYIFLDHIPSNVVAWITVRNYGFCDATEI
                     FVFIAGYSATLAYGSQMRRHGFLYGSASLLQRAWQLYIAHIFLFVLFTAQIGWVETRF
                     RNPMYSEEMNLLGFLSEPHVNLIQALLLRFRPTNMDVLPLYIALLLGFAPLLWLLLHR
                     QWLVLGLSGLLYLGAGANNWNLAGYPPGRTWFFNPLCWQFLFVIGACCALSPAAMAVL
                     TRQRRWLQPLCIAYLLFALAVVGTWYWPALETWLPAWLARLIYPIDKTNLDILRLLHF
                     LALAYLVTQRVGPDAAFLHGRLARPFVICGRHSLEIFCLGTLLAFTGHFYLTEFHGAL
                     PAQIAVSVAGTLLMILVAWIMEWYRNTGTQGRPSGASA"
BASE COUNT         1572 a         3923 c         3927 g         1546 t
ORIGIN      
        1 ggccggcgcg gctcgcggac ttcttcgccg cctggcgcac ggcgccggcc ggcttctggg
       61 tgctggccgg gctggcggtg ttcctgtcga tgatctggtt caccgatgcg gcgatccagt
      121 acgggctgtt cttcggcagc ggccgctggt cgccggccga cggcttcgat gcccgcctcg
      181 gcggctatgc ggccttcgcc ggcctcaccg gcggcgggct ggcgctggcg gcctggctgg
      241 tcaacggctt cgcgatgccg ctcacctgcc ggcgcggcct ggcgctgacc ccggcggtgg
      301 ccgccggcct gcgggcgctg cgcgcgcacc cgggcgtgct ggcgctgtgg gccggcgtgc
      361 tcggcggcgg cagcctcgcg accttcgcgc tgtgctatcc ggcgctggtc gtgaccgtgc
      421 cggtgctcgc ctacggcagc ctcgccgcct gcgaggagat ctttcccggc tccgggacct
      481 gaggcgactc gccgtcgacc agcgccggca cctcgaccag cacgcagcgg ttgcgcccct
      541 cgcgcttggc cgtgtacagc gcacggtcgg cctcggtgat cagcacatcg atcggcagat
      601 cgccgctgcc ggcggcgatg ccgatgctga tcgtgcagcg ccggcctccg agctcggctc
      661 ccgcatgctg ggccgcgacc gccgtgcgca ggcgctcgcc gatggtcagc gcggtacggg
      721 caccggtctg cggcagcagc gccgcgaact cctcgccgcc gaagcgtgcg agcagatcct
      781 cgcggcgcag gccactggcg agggtcgcgg acaccgtgcg cagcacggca tcgccaaccg
      841 gatggccgaa gctgtcattg atgatcttga agtgatcgag atcgagcatc agcaccgcgg
      901 ccggcgtctg cgggcgcagc gcctgcccgg cccggctcag gaaactgcgc cggttcagca
      961 gcccggtcag cgcatcggta ccggcgagcc gctgcaggtg ttcctcgtgg ccggcgcggg
     1021 cgagcagcac cagaccgatc gtgacgatga tggtgctggc gtagccgctc aggaagctcg
     1081 cctgttcgag gacggtgcgc cagatctcga cctgccgcca cagggcacgc gccagcagca
     1141 gttcggcact gcgcgcgatc aggacgcccc cgagcagcag cagcgcgagc gccatgatgc
     1201 tctgcgcggt cacgtgcccg tgatccggca ggcgccggac ctgccgggcg caggcgcagg
     1261 cgagcagccc gtacagcagc gagtgcaggc cgaccagcca ttcggaggcg ggcgacagca
     1321 gcgagatgcc ggcggacacc ccgaccagcg ccggcacccg ccagagcgtg ccgtcccgtc
     1381 cggcgcagcg atccagcgcc tgcaggcgca ggccccagca caggctcacg cagccgagcg
     1441 ccagcggccc ggcaaagccg gccgggatca gtgaattgct cgccaccagc acgaaccagc
     1501 cggcggcctg caccagcagc gacagcgtcc acagctgcag gccgctcagc gaacgctgcg
     1561 gcgcacgacc ctgatcggcc agcgtgatca cgcccgccat cagcagcgcg ctgagcgcca
     1621 tgaagaacat cagggtcttg acatcgaaca tcatcgggca tcctgccgtt cagcagacgg
     1681 cagcaatggc ggatgctgcg ccggcatccc gtccatcacc gcccgtcatc ctgcatcggc
     1741 attcctgcgg acggctttga tgcagatcgc ctccgcggcc ccagcgacgg ctccgcacct
     1801 gccggcgcga gccagccggc acgtccggtg tacggtttcg cgccggccgg acctgccact
     1861 ggccgcacga ctcacttgcg tccgagcagc gccagcccct tcagcaggtt cagggcctcg
     1921 gcgagcgcgt agtcgctctg cacgagcggc agttcggtgg acctgccggc gccggccggc
     1981 gccgcctggg ccggatcggc ctcgccggcc ccgccggttt cgttgccgcg ctgcccgggg
     2041 ttgttcaggt ggccggcgag atcggcctcc ttgaccgtgt cggcggcctc gacgccggtg
     2101 acgcgcacgg cgtcgacgcg gatgtccggc tcgatgccct gggcctggat cgagcggccg
     2161 ctcggcgtgt agtagcgcgc cgtggtcagc ttcacggccg tgccatcggc gagcggcatg
     2221 atggtctgca ccgagccctt gccgaaggtg cgcgtgccca tgatcacggc gcggcgctgg
     2281 tcctgcagcg cgccggcgac gatctcggac gccgaggccg aaccgccgtt gaccagcacg
     2341 accagcggca cgccgtcgat caggtcgccg gcaccggcct ggaagttctg gcgcgagccg
     2401 gattcgcggc cctcggtgta gacgatcagc cccgagtcga ggaaggcatc agcgacctcg
     2461 acggcgcccg acagcacgcc gccggggttg ttgcgcaggt cgagcaccag gcccttgagc
     2521 ctgccgccgc cggcctgctt cagcgtcgcg agctgctcgc gcaggtagcc ggcggtgcgc
     2581 gactggaact gcgtgatgcg cacgtagccg tagccgggct ccagcgcgcg gctcttgacg
     2641 ctgcgcaccg tgatcacgtc gcgggtcagc gtgatctcca gcggcttgtc ctcgccggcg
     2701 cgcacgacgg tcagcttcac cgaggagccg accgagccgc gcatcagctt caccgaatcg
     2761 gcgaggccga ggcccttggt cggcgtgtcg tcgatgcgca cgatcaggtc gccggcgcgg
     2821 atgccggcgc gcgccgccgg cgtgtcgtcg atcggcgaga tgaccttgac gaagccgtcc
     2881 tcctgcccga cctcgatgcc gagcccgccg aactcgccgg tggtgccgac ctgcatctcg
     2941 cggtagccct ctttgtcgag gtaggacgag tgcggatcga ggccggcgag catgccgcgc
     3001 acggcgcttt cgagcagctt gcggtcatcg accggctcga cgtagtcgtg gcgcacgcgt
     3061 tcgaggatct cggtgaaggc gcgcagttcc tcgaacggca gcgccggcgg tgccgccgga
     3121 tccgcggccg acaccgcgcc gacccaggcg agaccggcgc cggcgatggc gccggtgagc
     3181 aacgacagag cgtggcgata cggaaacttc atgacgactc cgggtgcgcg gcgcgcgaca
     3241 gaacggacgg aacggacaaa acgaaggaag gccgccgcgg cggccccggc gcggtcgatt
     3301 ctggcgcgga tcgcggcccg atggcaggtg tcaggctgtt gcacggccat gcgggccggg
     3361 ctgcgccggg gagcctgcgc cccgcggggg aggagccggt tccggcccgg gccttgtgcg
     3421 tcggcccggg tcgtgcgccg gcgccgccac ccgcggggtg cagggcatgc ggaacgcgcg
     3481 gacggaaggg gatttcagtc cagcgccagc agcggccggc cggtcatctc cggcggctgc
     3541 ggcaggttca ggagtttcag cacggtcgga gccacgtcgc agagcgcgcc ggtgccggcc
     3601 ggcgtgccgc ggcgcccggc gtgcgagacg tacaggaagg gcacgacgtt ggtcgtgtgc
     3661 gcggtgttgg cctgcgcgct gtcggcgtcc cacatgctct cgacgttgcc gtgatcggcg
     3721 gtgaccagca tctcgccacc gacctcggcg agcgcggcat cgagccggcc gagcgcggcg
     3781 tcgatggcct cgaccgcctg cacggcggcg gcgtagaggc cggtgtggcc gaccatgtcg
     3841 gggttcgcgt agttgacgac gatcaggtcg aaggcgcgcg cgcggatcgc ctcgaccagc
     3901 cggtcggtga cctcgggcgc gctcatctgc ggctgcaggt cgtaggtcgc gaccttcggc
     3961 gacgggatca ggatgcgctc ctcgcccgga tacggcggct cgcgtccgcc gctgaagaag
     4021 aaggtcacgt gcgcatactt ttcggtctcg gcgatgcgca gctgcgtgcg gccgtgctgc
     4081 gcgagccagt cgccgagcac gttggagagg tcctcgggcg cgtaggcgac cggcgccggg
     4141 atcgacttct ggtactgcgt caggcacacg aagccggcga gggccggcgc gcgggcgcgc
     4201 gcgaagccgc tgaaatccgg ttcgacgaag gcgcgcgtca gctcgcgcgc ccggtcggcg
     4261 cggaagttca tgaacacgac cgcatcgccg tcggcgaccg tcaccggcgc gccgatcacg
     4321 gtcggcgcga cgaattcgtc gttctcgccg cgcccgtagg ccgcggccag cccggcggcg
     4381 gcatcggcgg ccgtgtgcgc gccccgggca tcgacgatca gttcatacgc cgcctgcacg
     4441 cgctcccagc ggttgtcgcg atccatcgcg tagtagcgtc cgaccagcgt cgcgatgcgc
     4501 gcgacgccgg tggccgcaca gacggcatcc atcgccgcga gcgaatcggc ggcgctgcgc
     4561 ggcggcgtgt cgcggccgtc gaggaaggca tgcacgtaca cgcgctgcgc accgcgtccg
     4621 gcggccagcc gcaccatcgc ctgcaggtgc tcctcgtggc tgtgcacgcc ccccggcgac
     4681 agcaggccca tgacgtgcac ggcaccgccc cccgcgaccg cggcatcgac ggccttgcac
     4741 agcaccgggt tttcgaagaa cccgccgtgc tcgatggcga ggccgatgcg ggtcagctcc
     4801 tgatacacga cgcggccggc gccgaggttc atgtgcccga cctcggagtt gcccatctgc
     4861 ccggccggca ggccgaccga cagccccgag gtgtcgatca gcgtgtgcgg gtgcgtggcc
     4921 cacagccggt cccagttcgg cgtatgcgcg ccgaggatcg cgttgtgggc cggatcctcg
     4981 ctgtaacccc agccgtcgag gatcgcgagg acgagggggc ggcgcgtggc gggcatggca
     5041 gggggttctc cgcggttgca ggatggatgg ccgtgccggg cgcacacgcc cggcacacct
     5101 aagggcgtga aaggataaag acttcccccc tgcggatgcc agccgcacgg tgcgcgcccg
     5161 gggcgcgtcc gggcgggtta caatcgccgt ccctcaccgc cgccccgccg ggtttccgcc
     5221 gcatgcagac ctttctcgac ttcgccgcgc gcaactggct gctgtttgcc gccttcctcg
     5281 cgatcctcgt cgccctgctc gtcaccgaat tcctgcgcct gtcccgcggc gtcaaggcga
     5341 tcggcgtgca ggatgcgctg cgccggctca acgacgagac cgccgtgctg gtcgacgtgc
     5401 gcgatcccgg cgaattccgc aacggccaca tcagcggcgc ccgccacctg ccggccggcg
     5461 agttcgaccg gcgcatcggc gagctcgaca agtaccgcga ccgcgaactc atcgtctgct
     5521 gccagcacgg caaccgctcg gcaccgctgg cggcgaagct gaagaaggcc ggcttcacgt
     5581 cggtcatgaa cctcgccggc ggcgtcgccg cgtggaccgg cgccggcctg ccgctggtgc
     5641 gcaagtgaac gcgccggcgc gccggcccga gatcatcgtc tacgcctcgc gctggtgccc
     5701 gtactgccgc gcggccttcg cgctgctcga tcgcaagggc gtcgcctacg aggtgctcga
     5761 tgtcgacgag cagccggcgc tgtggaacga gatcgacgcg cgcaccggcc gtgacaccgt
     5821 gccgcagacc ttcatcgacg gccaccacgt cggcggctac gacgacatgg ccgcactcga
     5881 tcgcgccggc ggcctcgacc cgctgctgtt cccgaaggcc cccggctgaa agcaagcccc
     5941 cgcccgcacg ccggcgcacg tgcttggccg taccgcagcg cgcgcgtata aagcctgacc
     6001 cgcccgggcg cacgccgtcc gcgccgccag caaccctcaa gaccctccaa gaccacggtc
     6061 cggaagccga cacccatgag cgagcagaac agcgccccgc agcagccgca gcagcacttc
     6121 gagatccaga agctgtacac gaaggacatc tccttcgaga ccccgaactc gccgatgatc
     6181 ttcaccgagc agtggaccgg cgagagccag ctgcagctgc acaacgaatc cgcgcagctc
     6241 gccgacaaca tccacgaggt cgtgctgacc gtcaccgtca ccaccaagca gggcgacaag
     6301 accgcctacc tgtgcgaagt ccgccaggcc ggcatcttca cgatcgccgg cttcgcaccc
     6361 gaccagctcg cggcgatgct cggtgcctac tgcccgaacg tgctgttccc gtacgcgcgc
     6421 gaggcggtct cgaacctgat ccagcgcggc ggcttcccgc cgctgcagct cgcgccggtg
     6481 aacttcgacg cgctgtacat gcagcacctg caggccaagc aggcgcagca gatgcaggcc
     6541 ggccagccga gcagcggcac ggcctgaccg gcctgctccg gctgaccctg ccccgcccgc
     6601 ccggcacctg atggccacgc tcgcggtact cggcgccggc tcctggggca cggcgctcgc
     6661 gctgctgctg gcgcgcaacg gtcatgccgt gcgcctgtgg ggccacgatc cggcgcaggt
     6721 cgcacggctc gccaccgaac gcgagaacgc cgccttcctg cccggcgtga cgctgcccgg
     6781 gcgcatcacg ccgggcgccg atctcgccgc cgcctgcgcc ggcgcggcga tggtgctggt
     6841 ggtcgtgccg agccacgcct tcgtcgcgac gctcgatgcg ctcgccgcgc tggcgcagcc
     6901 gccggcggcg ctcgcctggg ccaccaaggg gctggagccg cgcaccggac gcttcctgca
     6961 cgaggtcgcg cgcgagcgcc tcggcagccg gccgctggcg gtggtctccg gcccgaactt
     7021 cgcgcgcgag gtcgcgcgcg gcctgccgac cgcgaccacg gtcgcgagcc tcgacgccgg
     7081 ctgggcggcg acggtcgccg gcttcctgca cgggcgcgac ttccgcgcct acaccgtcac
     7141 cgacgtgagc ggcgtcgaac tcggcggcgc ggtcaagaac gtgctcgcga tcgcctgcgg
     7201 cgtcgccgac ggcctcggct acggcgccaa cgcccgcgcg gcgctgatca cgcgcggtct
     7261 ggccgagatg gtgcgcctcg gcctcgcctg cggcggccgg cgcgagacct tcatggggct
     7321 cgccggcgtc ggcgatctgg tgctcacctg caccgatgac cagtcgcgca accgccgctt
     7381 cggcctcgcg ctcgggcgcg gggccggcgt cggggaggca ctcgccggca tcggtcaggt
     7441 cgtcgagggc gcggccgcag tcgccgaagt cctgaagctc gcgcgcagcc acggtgtcga
     7501 gatgccgatc tccgagcagg tcgaggccgt cgtccacggc ggccgcccgc cggcggcggc
     7561 ggtcgaggcc ctgctggcgc gcgagccgcg cgatgacggg gtgtgaggac ggcccatgta
     7621 cggatggctg ctgctgctgc acatcctcgg tgccagcgtc tggaccggcg ggcacctcgt
     7681 gctgtcgctc gtgatcctgc ccggtgtgct gcgcgagcgc tcggcagcga agctgctcgc
     7741 gttcgaatcg ggcttcgaga agatcggcat gccggcgctg gtcgtgcagg tcgtcaccgg
     7801 cctgctgctc gcctggcggc agctgccgca gctgacggcg tggttcgatc cggccaatcc
     7861 gctcgcgcac gcgatccttg ccaagctcgg actgctgctc gcgaccttcc tgctcgcgct
     7921 cgatgccaaa ctgcgcgtgc tgccgcactt cgatgattcg cggctcaccg acatggcctg
     7981 gcacatcgtg ccggtgacgg tgctgtcggt gctgttcgtc gcggccggcg tgtcgtttcg
     8041 caccggctgg ctgtactgaa acgcctgcac cggcaatgcg cccgcccctg ccgcgcaccg
     8101 cctcgcggca gggacgcgcg ccggcttacg gcgccagcgt cgtcgccagc gtgccgccgg
     8161 tgctgccggc atcgagcgtg tcgatgccca tcgccacctg cgcggcgagg cagtggtagc
     8221 cgaggtcgtt ctgatgcagg ccgtcggcag tcagcatcgc cgccaacagc ggggcatcct
     8281 tcgcggcgag ctgctgcatc agttcgtagc gctgcacgac cggcacgtcg aactcgtcgc
     8341 cgagctgctc gatcagctcg acataggcct cgatctgctt cgcggctgcg gtctgcggcg
     8401 cgtactgcgg cggcatcagg atcacgtcga tgccggcact gcgcagcgcc gccagacctt
     8461 ccttgagcgt cgcggcgaac tgttcgagcg ggacgttgcg caccacgtca ttggcgccgg
     8521 cctgccagat caccagcgtc gggtcgttcg gatagacatc cttgccgaag cgcgccagcg
     8581 tctgcgtggc cacctcgccg ccgcggccct tgttgatgac ctcgatcggc tcgccgacgt
     8641 agcgcgtgcg cagcaggctg ccgagctggg ccggataggt gttggccggc gagctggcgc
     8701 cgtagccttc ggtcgacgag gagccgatcg cgacgatggt cagcggctcg cgccggtcga
     8761 tccgggcgcg cgtgcgctcc agcgcgccgt cgaggcgcac catctgcgcc ggcagcacgc
     8821 agcggcggtc gatcgacagc ttgccgcttt cggcacgcac cggcttcggg gcgagcagca
     8881 ccatcgccgc cagcaggccg ccgaggccgg cgagcgccag atgccgggcc ttgcggctgt
     8941 tgcgcggcag cgcgtggctg cgcgcgctcg gctcggagtg ggtgtgcttg cgcagggtat
     9001 cgggtcgcat cgggtgtctc cctgttatgg cgctgcgcct tcaccggcgc tcagcgtgaa
     9061 gcccggtcga tcatgtcggc caggtaccgg ccgagacagt cgtggatgcg atcggcaacc
     9121 cggcgctgtg actcggcgtc cgccggatcg aggtcgagca agccctgctc gacccaccgg
     9181 cgcatgattt cgaaacgcgg gaacagcgcg gcgcgccgtc ccatcgcgat ctggcgaagg
     9241 taacccagat aagggccggc atcatagaga acaaaggctt cgctgccgta ctgcgcgtcg
     9301 atcagcagcg tgtcggccgc ggcgttctcg gcgtactgca ggccgtgctc gatggcatcg
     9361 ccgaactcgt tgatgtcgac gccgcggatc gcatcgacgg tgccggtctg ccagaccagc
     9421 agttcggggt gataggtcgc gagcgcctcg ggcagcttct cgatctgctg ggcggcggtc
     9481 tggccggggc gggcgaggtt gacgacgtgc acgcgcctgt cgccccagcg cgcctccagc
     9541 cagtgctcca tgcgcaccgg atagctcagc gtcggcgagg tcgggccggc acgcccggcg
     9601 gtcgaggccg tgccgagcac gaggatggtc acgtcgcggt tctcaagccg cgccgcggcg
     9661 acgtgcgaca gcgggaacgg cgtgccgcgg atcagcgacg gatcgccgca ttcgctgact
     9721 tcgggcgcga ccggggaatc atcgtcctcg tccgccgacg ggacgggcgg cacgacctcg
     9781 gcatgcactc cggcaccgag cagcgcggcg agcaggaaca gcacgggacg gcggcagcgg
     9841 gggggagtca tgccgaagct ccggagggac ggccctgggt gccggtgttg cggtaccact
     9901 ccatgatcca ggcgacgagg atcatcagca gcgtgccggc gacgctgacg gcgatctgcg
     9961 ccggcagcgc accgtggaat tcggtcaggt agaagtggcc ggtgaacgcc agcagcgtgc
    10021 cgaggcagaa gatctccagc gaatgccggc cgcagatcac gaacggccgc gccagccggc
    10081 cgtgcaggaa cgccgcatcc gggccgacgc gctgggtcac gaggtatgcg agcgccagaa
    10141 agtgcagcag gcgcaggatg tcgaggttgg tcttgtcgat cgggtagatc agccgcgcca
    10201 gccaggccgg cagccaggtc tccagcgccg gccagtacca ggtgccgacc accgccagcg
    10261 cgaacagcag ataggcaatg cacagcggct gcagccagcg gcgctgtcgc gtcagcaccg
    10321 ccatcgccgc cggactcagc gcgcagcagg ccccgatcac gaacaggaac tgccagcaga
    10381 gcggattgaa gaaccaggtc cgcccgggcg gatagccggc gaggttccag ttgttggcac
    10441 cggcgccgag gtacagcagc cccgacagtc cgagcaccag ccactggcgg tgcagcagca
    10501 gccacagcag cggcgcgaag ccgagcagca gcgcgatgta cagcggcagc acgtccatgt
    10561 tggtcggccg gaagcgcagc agcagcgcct ggatcaggtt cacgtgcggt tcgctgagga
    10621 agccgagcag gttcatttcc tccgagtaca tcggattgcg gaagcgcgtt tccacccagc
    10681 cgatctgcgc cgtgaacagc acgaacagga agatgtgcgc gatgtacagc tgccaggcgc
    10741 gctgcagcag gctggcgctg ccgtacagga agccgtggcg gcgcatctgg ctgccgtagg
    10801 cgagcgtcgc gctgtagccg gcgatgaaca cgaagatctc ggtcgcatcg cagaagccgt
    10861 agttgcggac cgtgatccac gcgaccacgt tcgacgggat gtgatcgagg aagatgtaga
    10921 tcagcgccag cccgcggaag aaatccagcc gggcgtcgcg accggcgg
//
LOCUS       DAOQIE010000108        10907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3002_length_10907_cov_7.580538, whole genome shotgun sequence.
ACCESSION   DAOQIE010000108 DAOQIE010000000
VERSION     DAOQIE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_10907_cov_7.580538"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PK144_08415"
     CDS             <1..978
                     /locus_tag="PK144_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF65788.1"
                     /translation="PGEGTLLLFRAEPWGDADRQLLALLAGSYAQARRLARLAARKSL
                     LARLPRGRRAWYWASALLVLALLAAWPIRQSVLAPAELVPRLPALVRAPVDGVVESFQ
                     IEPNQRVSAGQPLLRLDTTEVRSKLEVATRAREVALAEFQTASQQAFSDARAKATLAT
                     LAGRVEQQAAERDYYATLLERLQLRAPQDGIAIFDDPNDWLGRPVAVGQKILVVARPE
                     DIELEVHLPASDPLLLAAGDALLFFPNVAPDRPLGARLRVIGYTAVPGADGVAAYRLK
                     ASLDAGRTGNAADLRPGLRGTARVYGADTTLGAWLLRRPLAVLARWLNL"
     gene            984..3110
                     /locus_tag="PK144_08420"
     CDS             984..3110
                     /locus_tag="PK144_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF65789.1"
                     /translation="MSPATTGSASLPPLREELKLYPGPPARDGAPGWTLHDPATNRYV
                     RLGWLEIEFLRRWALGSPEAVLAAVRAETTLTAAAADLDAFVRFLADQQLLAIGHADT
                     SRLHAERRARLQRGWASWLLHHYLFLRVTLLRPDALLRAMQPWLAWVWTRACALTVAV
                     LALGGLLAILGRWERFTHEFPFDFDLRGALAVGACLAVSKVLHEFGHACTLHHYGCRV
                     PAMGVAFIVFWPVCWTDASAAWQLVERRKRLAIGAAGVLVELALAAIASWLWLLLPDG
                     ALRNGMHVLAGTAWLLTLAVNLNPLMRFDGYHLLADALDEPNLQARSFALGRWRLREA
                     LFGLGEAPPERLPAARRRLLVAYAWATWVYRFGLFVGIALAVYHYFFKALGIALMLVE
                     LGWFIALPLYSELRAWHERRARLRHWRAWRVPMLLLAALAALLLLPWHGSVSAPAVLL
                     AAREVQVFAGQPGRVQKLAVAEGSTVAAGERLLVLDNPDLEHRIATLTQQLAVLEAVT
                     ATHTLDARLNDRNPIERQELLRVATELEGARAQAARLTVRAPWPGRVADLAPDLREGA
                     WLGAAEPLLTLVGTGAPKAVGYVAEADLHRIAVGARASFHASDRLRGLRLRITRIEPS
                     TAQRLPELALASPLGGDLAARLDEQQSAIPIEARYRIELQPEDASLPPPSPPLPGELS
                     IETPAEALIVRLWREVAGALVRESGW"
     gene            complement(3154..3846)
                     /locus_tag="PK144_08425"
     CDS             complement(3154..3846)
                     /locus_tag="PK144_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007352454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNF65790.1"
                     /translation="MFEREHHRRIASVLAALDATLLEQSRCWFGGGTAIAMQLGEYRE
                     SVDIDFLCADRDGYRLLRSAVFGRGVQGLMRYPVRELRESRADQYGIRSVLEVEGVPI
                     KFEIVREARIDLAGSQVSGMPVGCVDRMGLFAEKLLANVDRGLDRSTLSRDALDLIVM
                     QQAWGDAGELPKSAWMLAESAYGDAIGKGWRVAIERLRDEPDYRVRCLDALDIKPDAR
                     TCIEDFLGRSRS"
     gene            complement(3839..4066)
                     /locus_tag="PK144_08430"
     CDS             complement(3839..4066)
                     /locus_tag="PK144_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65791.1"
                     /translation="MNGENAQAPQTVRVADYPQLRLLCWNRRDAVLAAAEAFALYEAN
                     WRHVDAATLDAQERVLIEALVREYGRGVLNV"
     gene            complement(4102..5355)
                     /locus_tag="PK144_08435"
     CDS             complement(4102..5355)
                     /locus_tag="PK144_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNF65792.1"
                     /translation="MSIESGDTFLRIGLSVALGLLIGLQREATGPEFAGLRSFALTAL
                     LGTLCGLLAERFGGWVLVAGLAAITTLLVLGNVMQWRGRVDDLEPSVTTELALLLMFG
                     LGAYLAFGAWEVAVVVAGLSVVLLHFKVELHALARRLGGEDLRAIMQFVLITFIVLPV
                     LPNRSFGPLEVFNPFEIWLMVTLIVGISLGGYLLYKAFGGGAGVLLGGLLGGAVSSTA
                     TTLAYARRVRDGLAAPLAAVAIALASAVMLVRVVLIVALLAPALALALLPAAGAMLAA
                     TLVPVAWHWRRSRRQDNAMPAQKNPAELGPAMLIAALYALILFVLAAARRYLGHDALY
                     LVSVVAGLTDVDAIVLSVTRFAAEGSLPEVLGWRLIVTAVLSNAIFKLTLAASLGGRE
                     VLLRTLVLLAPSLVIGTGMVVAWPD"
     gene            complement(5384..6202)
                     /locus_tag="PK144_08440"
     CDS             complement(5384..6202)
                     /locus_tag="PK144_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65793.1"
                     /translation="MNARLRAAAALLLAALLLAMSPARAEVPVLVTVDLRHRNAEELI
                     PLLRPVAGELTLSGQGAVLIVRGPQARIDELLGVLDALDVPPRTLLVSLRQGSIDEVD
                     ASGVGLEGGIGTGASGTTASGRVTVEHYGTRGRDDTVQTVRVLDGREAFVTLGRSVPV
                     PERYGVATGPRGGVAAGVDMGYRDASTGFLVRPRVSGETVTVEVAPRSVRPGPDGSFD
                     TASLSTTLSGRLGQWLLIGGSAEARRAARDAVVHSTRSRSGSERVWLLKVEPAP"
     gene            complement(6199..6510)
                     /locus_tag="PK144_08445"
     CDS             complement(6199..6510)
                     /locus_tag="PK144_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF65794.1"
                     /translation="MYDPRNLKSLKLLAQYAPEAMAAYRGLQHAAAAEGALDGKAKAL
                     IALACAHVARCPYSIDTRLAEARAAGAHEAEIAEAVLVAAAVQAGATVMHGSHCYGTP
                     E"
     gene            complement(6565..6927)
                     /locus_tag="PK144_08450"
     CDS             complement(6565..6927)
                     /locus_tag="PK144_08450"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNF65795.1"
                     /translation="MANQPHRGLRRIVNATGYSWAGLKAAWHNEEAFRQESLLALCLT
                     PLALWLGDTSLERALLIGSLLLIVIVELLNSGIEAVVDRIGTERHELSGRAKDIGSAA
                     VFVSLLNAAVVWGLILFG"
     gene            complement(6946..7446)
                     /gene="tpx"
                     /locus_tag="PK144_08455"
     CDS             complement(6946..7446)
                     /gene="tpx"
                     /locus_tag="PK144_08455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNF65796.1"
                     /translation="MATITLRGNPIHTNGELPAAGTRAPDFHLVAPGLADVHLENYAG
                     KKKLLNIVPSLDTPTCQLSTKKFNDHAKAHPDTVILIVSADLPFAQGRFCTGEALDNV
                     IPLSMMRSRNFAKDYGVLIEDGPLAGITARAVVVLDENDTVLYAQLVPEIADEPDYAA
                     ALAALA"
     gene            complement(7535..7915)
                     /gene="gcvH"
                     /locus_tag="PK144_08460"
     CDS             complement(7535..7915)
                     /gene="gcvH"
                     /locus_tag="PK144_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNF65797.1"
                     /translation="MNPSELLYTKEHEWLRAEDDGSYTLGVTEYAQKQLGDIVFCEQP
                     VIGAKFEAEEPVGTLESVKAVAEVYVPVGCEITAINEALNDSPELVNEDPYGEGWLFR
                     LKATAAPEGLLSADAYEQYLQSAE"
     gene            8414..9634
                     /locus_tag="PK144_08465"
     CDS             8414..9634
                     /locus_tag="PK144_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF65798.1"
                     /translation="MTASTRSAPGYWSIALAAAAILAITMGARQTLGLFVAPLDASTG
                     LGVASISFALAIGQFVWGASQPVFGALADHYGPGRALVAGGVLLALGMALTPFMQSEP
                     GLILTLGVLAAAGAGAGSFSILIGAAAGLLPPARRAFASGFINAGGSFGQFVFAPFLQ
                     VLIAGFGWMQAMLTMAVLTLATIPLAWPLRRTPEQAAAHASTEGIGLGRQLRIAFADR
                     SYWCLHAGFFTCGFHIAFLVTHLPGEVGLCGLPASVSAAALAIIGLANIAGSLAAGSL
                     SQRYRMKHLLALMYASRAVIVALYLLAPKTPLTFYVFAAALGFTWLATVPPTAGLVGK
                     LFGVRYLATLFGMTLLSHQIGGFFGAWLGGLSRVGFGDYQWMWYADIVLALLAALANL
                     PIRENRPLPAAAPA"
     gene            complement(9631..>10907)
                     /locus_tag="PK144_08470"
     CDS             complement(9631..>10907)
                     /locus_tag="PK144_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65799.1"
                     /translation="NFRDTGRSWVVIDADPDLRRVAAVFDPQRGTVVPFAQALAQSFG
                     VPVERLGDRLNGHTFGIELDGELFVRSVAAIDTGTGALSFFCDVNPGDELKLLAATDF
                     VAQTKRDVQDFLRGKPKPLAVLMNDCVLRRLNNEPRLADLAGLWPVPAAGFSTFGELF
                     GINVNQTLSAVAFFAPGPSPFTDDLVDRFPVHYARFREYFTACRLRRAESLNGLRARV
                     LAHLLGQFEANTALAGEVAEALAQTAGIRSTVEAIRGALLTELATAGDNADVQALAEG
                     FASFERHTQGFRDVLKTIDRVASQTNLLALNATIEAVRAGEAGRGFAVVATEVKKLAN
                     DTRASLGRTEEVIRSMTAALGELGTHIDSARNRFGALHAHQQGTVGQVETIVANAGLI
                     ERALAGLGERAGAQRAAMAELAGDLDLLRRLD"
BASE COUNT         1528 a         3911 c         3858 g         1610 t
ORIGIN      
        1 cccggcgagg gcacgctgct gctgttccgg gccgagccct ggggtgatgc cgaccgccag
       61 ctgctggccc tgctcgccgg cagctacgcg caggcgcggc ggctcgcgcg gctggcggcc
      121 cgcaagtcgc tgctcgcgcg gctgccgcgc ggccggcgcg cgtggtactg ggcatcggcg
      181 ctgctggtgc tggcgctgct cgcggcgtgg ccgatccgcc agagcgtgct ggcgccggcc
      241 gagctggtgc cgcggctgcc ggcgctggtg cgcgcgccgg tcgacggcgt cgtcgagtcc
      301 ttccagatcg aaccgaacca gcgcgtcagc gccggccagc cgctgctgcg gctcgacacc
      361 accgaggtgc gcagcaagct cgaagtcgca acgcgcgcgc gcgaggtcgc gctcgccgaa
      421 ttccagaccg cctcgcagca ggccttcagc gatgcgcgcg ccaaggcgac gctggcgacg
      481 ctggccgggc gcgtcgaaca gcaggccgcc gagcgcgatt actacgcgac gctgctcgaa
      541 cgcctgcagc tgcgcgcgcc gcaggacggc atcgcgatct tcgacgaccc gaacgactgg
      601 ctcggccggc cggtcgcggt cgggcagaag atcctcgtgg tcgcgcgccc cgaagatatc
      661 gaactcgaag tccacctgcc ggccagcgat ccgctgctgc tcgccgccgg cgacgcgctg
      721 ctgttcttcc cgaacgtcgc cccggaccgg ccgctcggcg cgcggctgcg cgtgatcggc
      781 tacacggccg tgcccggcgc cgatggcgtc gccgcctacc ggctgaaggc cagcctcgat
      841 gccggccgca ccggcaatgc cgccgacctg cgccccggcc tgcgcggcac cgcccgcgtc
      901 tacggtgccg acaccacgct cggcgcctgg ctgctgcgcc ggccgctggc ggtgctggcg
      961 cgctggctga acctgtgagc cttgtgagcc cggccacgac gggcagcgcg agcctgccgc
     1021 cgctgcgcga ggagctgaag ctgtatccgg ggccaccggc gcgcgacggc gcgccgggct
     1081 ggacgctgca cgacccggcc accaaccgct acgtgcgcct cggctggctg gagatcgagt
     1141 tcctgcgccg ctgggcgctc ggttcgcccg aggccgtgct cgccgcggtg cgggccgaga
     1201 cgacgctgac ggccgccgcc gctgatctcg atgcgttcgt gcgcttcctc gccgatcagc
     1261 agctgctcgc catcggccat gccgacacca gccgcctgca cgccgagcgg cgcgcacggc
     1321 tgcagcgcgg ctgggcgagc tggctgctgc atcactacct gttcctgcgc gtgacgctgc
     1381 tgcggcccga tgccttgctg cgcgcgatgc agccgtggct ggcctgggtg tggacgcgcg
     1441 cctgcgcgct gacggtcgcg gtgctggcgc tcggcgggct gctggcgatc ctcggccgct
     1501 gggagcgctt cacgcacgaa ttcccgttcg acttcgatct gcgcggcgcg ctggcggtcg
     1561 gcgcctgcct cgcggtctcc aaggtgctgc acgagttcgg ccacgcctgc acgctgcacc
     1621 attacggctg ccgcgtgccg gcgatgggcg tggccttcat cgtgttctgg ccggtgtgct
     1681 ggaccgacgc cagcgccgcc tggcagctgg tcgaacgccg caagcgtctg gcgatcgggg
     1741 ccgccggcgt gctggtcgag ctggcgctcg cggccatcgc ctcatggctc tggctgctgc
     1801 tgccggacgg cgcgctccgc aacggcatgc acgtgctcgc cggcaccgcc tggctgctga
     1861 cgctggccgt gaacctgaat ccgctgatgc gcttcgacgg ctatcacctg ctcgccgatg
     1921 cgctcgacga gccgaacctg caggcgcgtt ccttcgcgct cgggcgctgg cggctgcgcg
     1981 aggcgctgtt cggcctcggc gaagcaccgc ccgagcggct gccggcggcg cgccgccggc
     2041 tgctggtggc gtatgcgtgg gcgacctggg tgtaccgctt cgggctgttc gtcggcatcg
     2101 cgctcgcggt ctaccactac ttcttcaagg cgctggggat tgcgctgatg ctggtcgaac
     2161 tcggctggtt catcgcgctg ccgctgtact cggagctgcg cgcctggcac gaacgccgcg
     2221 caagactgcg ccactggcgg gcctggcgcg tgccgatgct gctgctcgcg gccctcgcgg
     2281 cgctgctgct gctgccgtgg cacggctcgg tgtcggcgcc ggccgtgctg ctggcggcgc
     2341 gcgaggtgca ggtgttcgcc ggccagccgg ggcgcgtgca gaagctggcg gtcgccgaag
     2401 gctcgacggt cgcggccggc gagcggctgc tcgtgctcga caatcccgat ctcgaacacc
     2461 gcatcgcgac gctgacgcag cagctcgcgg tgctcgaagc cgtgaccgcg acgcacacgc
     2521 tcgatgcgcg cctgaatgac cgcaatccga tcgagcgtca ggagctgctg cgcgtcgcca
     2581 ccgaactcga aggcgcgcgc gcgcaggccg cgcggctgac ggtgcgcgcg ccgtggccgg
     2641 gccgcgtcgc cgacctcgcg cccgacctgc gcgaaggcgc gtggctcgga gccgccgagc
     2701 cgctgctgac gctggtcggc accggcgcac cgaaagccgt cggctacgtc gccgaggccg
     2761 acctgcaccg catcgccgtc ggcgcgcgtg cgagcttcca cgccagcgac cgcctgcgcg
     2821 gcctgcggct gcgcatcacg cgcatcgaac cgtcgaccgc ccagcgcctg ccggaactgg
     2881 cactggcctc accgctcggc ggcgacctcg ccgcccgcct cgacgagcag cagagcgcca
     2941 tcccgatcga ggcccgctac cggatcgaac tgcagcccga ggatgcctcc ctgcccccgc
     3001 cctcaccgcc gctgcccggc gaactgtcga tcgaaacccc ggccgaggca ctgatcgtgc
     3061 ggctgtggcg ggaggtggcg ggggcgctgg tgcgggagag cgggtggtga ccggatggct
     3121 gcagcgaagg aattcccggc aagcgccttt tcgtcaggac cggcttctgc caaggaagtc
     3181 ctcgatgcat gtacgcgcgt ccggtttgat atccagcgca tccaggcagc ggacacgata
     3241 gtccggctca tcccgcagtc gctcgatcgc cactcgccag cctttgccga ttgcatcgcc
     3301 ataggccgat tctgcgagca tccatgccga tttcgggagt tcgccagcat caccccatgc
     3361 ctgctgcatg acgatcagat ccagcgcatc ccgactcagc gtgctgcgat ccagcccccg
     3421 atcgacgttg gccagcagct tttcggcaaa cagccccatc cggtccacgc atcccaccgg
     3481 catgccagat acctgactac ccgccagatc aatgcgggcc tcgcgcacaa tctcgaattt
     3541 gatgggcaca ccctcgactt cgagaacgct gcggatgccg tattgatcgg ctctggattc
     3601 gcgcaactcc ctgacgggat aacgcatgag cccctgcaca cctcgcccga acacggctga
     3661 acgcagcagc cggtatccgt cgcggtctgc acagaggaaa tcgatgtcga ccgattcacg
     3721 gtattcaccg agctgcatgg cgattgctgt tccgccacca aaccagcaac gtgactgttc
     3781 gagcaacgtg gcatcgagtg ctgcgagcac gctggcgatg cggcgatggt gttcacgctc
     3841 aaacattcaa cacccccctg ccgtattccc ggaccagtgc ctcgatcagc acccgctcct
     3901 gcgcatcgag cgttgccgca tcgacatggc gccagttggc ctcgtacagc gcgaaggcct
     3961 cggcggcggc cagtacggca tcccggcggt tccagcacag caggcgcagt tgcggatagt
     4021 cggcgacccg cacggtctgc ggcgcctggg cgttttcccc gttcatcgcg gcttctcctg
     4081 tcgaagcttc cgatccggcc atcagtccgg ccacgccacg accatgccgg taccgatcac
     4141 cagtgacggt gcgagcagca ccagcgtgcg cagcagcacc tcgcgaccgc cgagcgaggc
     4201 ggcgagcgtc agcttgaaga tggcgttcga cagcaccgcg gtgacgatca gccgccagcc
     4261 gagcacctcc ggcagcgagc cttcggccgc gaagcgcgtc accgacagca cgatggcatc
     4321 gacatcggtc aggccggcga cgaccgatac caggtacagc gcgtcgtggc cgaggtagcg
     4381 gcgcgcggcg gcgagcacga acaggatcag cgcgtacagc gccgcgatca gcatcgccgg
     4441 gccgagttcg gccgggttct tctgcgccgg catggcgttg tcctgccggc gcgagcggcg
     4501 ccagtgccac gcgaccggca cgagcgtcgc ggcgagcatc gcgccggcgg ccggcagcag
     4561 cgccagcgcc agcgccgggg cgagcagcgc gacgatcagc acgacgcgca ccagcatcac
     4621 agccgaggcc agcgcgatcg cgaccgcggc gagcggtgcg gcgagcccgt cgcgcacgcg
     4681 ccgggcgtag gcgagcgtgg tcgccgtgct cgataccgcg ccgccgagca ggccgccgag
     4741 cagcacgccg gcaccgccgc cgaaggcttt gtagagcagg tagccgccga ggctgatgcc
     4801 gacgatcagc gtcaccatca gccagatctc gaacgggttg aacacttcga gcgggccgaa
     4861 gctgcggttc ggcagcaccg gcagcacgat gaaggtgatc agcacgaact gcatgatcgc
     4921 gcgcaggtcc tcgccgccga gccggcgcgc cagcgcgtgc agttcgacct tgaagtgcag
     4981 cagcacgacg ctcagaccgg caacgacgac cgcgacctcc caggcgccga aggcaagata
     5041 agccccgagc ccgaacatca gcagcagcgc gagttcggtg gtcaccgacg gttcgaggtc
     5101 atcgacgcgc ccgcgccact gcatgacgtt gccgagcacc agcagcgtcg tgatcgccgc
     5161 cagccccgcc accagcaccc agccgccgaa gcgctcggcg agcagtccgc acagcgtgcc
     5221 gagcagcgcc gtcagcgcga agctgcgcag gcccgcgaac tccgggccgg tcgcctcgcg
     5281 ctgcaggccg atcagcaggc cgagcgcgac cgacaggccg atgcgcagga aggtgtcgcc
     5341 ggactcgatg ctcatcggcc cgggccggcg tcatgcgctg cgatcacggc gccggctcga
     5401 ccttcagcag ccagacgcgc tcgctgccac tgcgcgagcg ggtcgaatgc acgacggcat
     5461 cgcgcgcggc gcggcgcgcc tcggccgagc cgccgatcag cagccactgg ccgagccggc
     5521 cgctcagcgt cgtgctgagg ctcgcggtat cgaaactgcc atcgggcccg ggacgcaccg
     5581 aacgcggcgc gacctcgacc gtcaccgtct cgccgctgac gcgcgggcgc accagaaagc
     5641 cggtcgaggc atcgcggtag cccatgtcga cgccggccgc cacgccgccg cgcgggccgg
     5701 tcgcgacgcc gtagcgctcg ggcaccggca ccgagcgccc gagcgtgacg aaggcctcgc
     5761 ggccatcgag cacgcgcacg gtctgcacgg tatcgtcgcg cccgcgcgtg ccgtagtgct
     5821 cgacggtcac ccgcccgctc gccgtcgtac cgctggcgcc ggtgccaatg ccgccttcca
     5881 gcccgacgcc ggaggcatcg acctcgtcga tcgagccctg acgcaggctg acgagcagcg
     5941 tgcgcggcgg cacgtcgagg gcgtcgagca cgccgagcag ttcgtcgatg cgcgcctgcg
     6001 ggccgcgcac gatcagcacg gcgccctgac cgctcagcgt cagctcgccg gcgaccgggc
     6061 gcagcagcgg aatcagctcc tcggcgttgc ggtgcctgag atcgaccgtg accagcaccg
     6121 gcacctcggc ccgggccggc gacatggcca gcagcagcgc ggccagcagc agcgcggccg
     6181 cggcgcgcag ccgcgcgttc attccggcgt gccgtagcag tgcgatccgt gcatgacggt
     6241 ggcgccggcc tgcaccgcgg cggcgaccag cacggcctcg gcgatctcgg cctcgtgcgc
     6301 gccggcggcg cgcgcctcgg ccagccgcgt gtcgatgctg tacgggcagc gcgcgacgtg
     6361 tgcgcaggcg agcgcgatca gcgccttggc cttgccgtcg agcgcgcctt cggcggcggc
     6421 ggcgtgctgc aggccacggt atgcggccat cgcttccggt gcgtactgcg cgagcagctt
     6481 caggcttttg agattgcggg ggtcatacat ggtggcgcga ctccggcccg gggacaggcc
     6541 gcaagcgtag gccgcgccgg gccgtcagcc gaacaggatc aggccccaga ccacggcggc
     6601 gttgagcagc gacacgaaca ccgccgccga gccgatgtcc ttggcgcggc cggagagctc
     6661 atgacgctcg gtgccgatgc ggtcgacgac cgcctcgatg ccggaattca gcagctcgac
     6721 gatgacgatc agcagcaggc tgccgatcag cagcgcgcgt tcgagcgagg tatcgccgag
     6781 ccacagcgcg agcggcgtga ggcacagcgc gagcaggctt tcctgccgga aggcctcctc
     6841 gttgtgccag gcggccttca gccccgccca cgaatacccg gtggcattga cgatgcggcg
     6901 caggccgcga tggggctgat tggccaatcg gcgtgaccgc gcgcgtcagg cgagcgccgc
     6961 gagcgcggcg gcgtagtcgg gctcgtcggc gatctcgggc acgagctgcg cgtacaggac
     7021 cgtatcgttc tcatcgagca cgaccacggc gcgtgcggtg atgccggcca gcgggccgtc
     7081 ctcgatcagc acgccgtagt ccttggcgaa gttgcgcgaa cgcatcatcg acagcgggat
     7141 gacgttgtcg agcgcctcgc cggtgcagaa gcgcccctgc gcgaacggca ggtcggccga
     7201 gacgatcagg atcacggtgt ccggatgcgc cttggcgtgg tcgttgaact tcttggtcga
     7261 cagctggcag gtcggcgtgt cgaggctcgg cacgatgttc agcagcttct tcttgccggc
     7321 gtagttttcg agatgcacgt cggcgaggcc gggagcgacc aggtggaagt ccggtgcgcg
     7381 cgtgccggcg gccggcagct cgccgttggt gtggatcggg ttgccccgca gcgtgatcgt
     7441 ggccatgagc ggcgtctcct gatggtgggt gaggtgatgt gccgcccggc gggcgggcgg
     7501 cgactccggc tctgcggccg gtacgggcag cggcttattc ggcgctctgg aggtactgct
     7561 cgtaggcatc ggcgctcagc agcccctcgg gggccgccgt cgccttgaga cggaacagcc
     7621 agccctcgcc gtacgggtcc tcgttgacca gttcggggct gtcgttcagg gcctcgttga
     7681 tggcggtgat ctcgcagccg acgggcacgt acacctcggc gacggccttc acggattcga
     7741 gcgtgccgac cggttcctcg gcctcgaact tcgctccgat caccggctgt tcacagaaca
     7801 cgatgtcgcc gagctgcttc tgtgcatatt cggtaacgcc gagcgtatag ctgccgtcgt
     7861 cttcggcgcg cagccattca tgttccttcg tgtacagcag ttcgctgggg ttcatggcgg
     7921 acactctcct gtgcgcgggg gcgcggcggg gatgacggga agggtcggaa ggcgggcggg
     7981 gtccggccaa agccggcagg ccgagactat atgcagacgc agcaggggct gccagcgtgc
     8041 gccggactga aaaacgggcg cgcggatgac gaatgaatga cggggcggag gcctggcgaa
     8101 tgacaaaaaa gaagggcgtg cgtccgccga cgcacgccct caatgaggta gagagagatg
     8161 agcgcgccgc gcggtgcctg ccaccgtgtc gcgaactcaa gacaaatgat aatgattctc
     8221 gattgatccg gcaagcatgg atcaacgccg gatcacgccg gcagcgcttc gtgcgtgcat
     8281 ggcagccatc gctgcgcccg actggtcagg ctgccggcgg atttccatag tgcggcaccc
     8341 gaatccgtct gatctgtccg gtgcagcccg tggcgcgcac cgctgcccgc cgtctgcccg
     8401 cgaggacccg cccgtgaccg cttccacccg ctccgccccc ggctactgga gcatcgcgct
     8461 cgccgccgcc gcgatcctcg cgatcacgat gggcgcgcgc cagacgctcg gcctgttcgt
     8521 cgcgccgctc gatgcctcga ccgggctcgg ggtcgcgtcg atcagcttcg cgctggcgat
     8581 cggccagttc gtctggggcg cctcgcagcc ggtgttcggg gcgctggccg atcactacgg
     8641 gccgggccgg gcgctggtgg ccggcggcgt gctgctcgcg ctcggcatgg cgctgacgcc
     8701 gttcatgcag agcgagccgg gcctgatcct gacgctcggc gtgctggccg cggccggagc
     8761 cggggccggc agcttctcga tcctgatcgg tgcggccgcc ggcctgctgc cgccggcacg
     8821 aagggcgttc gcgtccggct tcatcaatgc cggcggctcg ttcgggcagt tcgtgttcgc
     8881 gcccttcctg caggtgctga tcgccggttt cggctggatg caggcgatgc tgacgatggc
     8941 ggtgctgacg ctggcgacga tcccgctcgc gtggccgctg cggcgcacgc ccgagcaggc
     9001 ggccgcacac gcctcgaccg agggcatcgg cctcggccgg cagctgcgca tcgccttcgc
     9061 cgatcgcagc tactggtgcc tgcatgccgg attcttcacc tgcggcttcc acatcgcctt
     9121 cctggtcacg cacctgcccg gcgaggtcgg gctgtgcggg ctgccggcga gcgtgtcggc
     9181 ggcggcgctg gcgatcatcg ggctcgccaa catcgccggc agcctcgccg ccggctcgct
     9241 gtcgcagcgc taccgcatga agcacctgct ggcgctgatg tacgcctcgc gcgccgtgat
     9301 cgtcgcgctg tacctgctgg cgccgaagac gccgctgacc ttctacgtgt tcgccgccgc
     9361 gctcggcttc acctggctcg cgaccgtgcc gccgaccgcc ggtctggtcg gcaagctgtt
     9421 cggcgtgcgc tatctggcga cgctgttcgg catgacgctg ctgtcgcacc agatcggcgg
     9481 cttcttcggc gcctggctcg gcggcctcag ccgcgtcggt ttcggcgact accagtggat
     9541 gtggtacgcc gacatcgtgc tcgcgctgct cgcggcactc gcgaacctgc cgatccgcga
     9601 gaaccgcccg ctgccggcgg cggcaccggc ctaatcgagc cggcgcagca ggtcgaggtc
     9661 gccggcgagt tccgccatcg ccgcgcgctg cgccccggcg cgctcgccga gcccggccag
     9721 cgcgcgctcg atcaggccgg cgttggcgac gatggtctcg acctggccga ccgtgccctg
     9781 ctggtgcgca tgcagcgcgc cgaagcggtt gcgcgccgag tcgatgtggg tgccgagttc
     9841 gccgagcgcg gccgtcatgc tgcggatgac ttcctcggtg cgcccgagcg aggcgcgcgt
     9901 gtcgttggcg agcttcttga cctcggtcgc gaccaccgcg aagccgcgcc cggcctcgcc
     9961 ggcgcgcacg gcctcgatcg tcgcgttgag cgccagcagg ttggtctggc tggcaacgcg
    10021 gtcgatggtc ttcagcacgt cgcggaaacc ctgcgtgtgc cgctcgaagc tcgcgaagcc
    10081 ctcggccagc gcctgcacgt cagcgttgtc gccggcggtt gccagctcgg tcagcagcgc
    10141 gccgcggatg gcttcgacgg tcgagcggat gccggcggtc tgcgccagcg cctcggcgac
    10201 ctcgccggcg agcgcggtgt tggcctcgaa ctggccgagc agatgggcga gcacgcgcgc
    10261 gcgcaggccg ttcagggact cggcgcggcg cagccggcag gccgtgaagt actcgcggaa
    10321 acgcgcgtaa tgcaccggga agcgatcgac gagatcgtcc gtgaacggtg acggccccgg
    10381 tgcgaagaag gccaccgcgc tcagcgtctg gttgacgttg atgccgaaca gctcgccgaa
    10441 ggtcgagaac ccggccgccg gcaccggcca cagaccggcg agatcggcga ggcgcggctc
    10501 gttgttgagc cggcgcagca cgcagtcgtt catcagcacg gccagcggct tcggcttgcc
    10561 gcgcaggaag tcctgcacgt cgcgcttcgt ctgcgcgacg aaatcggtcg ccgccagcag
    10621 cttcagttcg tcgccgggat tcacgtcgca gaagaacgac agcgccccgg tgccggtgtc
    10681 gatcgccgca accgagcgca cgaacagctc gccgtcgagc tcgatgccga aggtgtggcc
    10741 gttgagcctg tcgccgagcc gctccaccgg cacgccgaag gactgcgcca gcgcctgcgc
    10801 gaacggcacg accgtgccgc gctgcggatc gaacaccgcg gctacccggc gcaggtccgg
    10861 atcggcgtcg atgaccaccc agctgcgacc ggtgtcgcgg aagttct
//
LOCUS       DAOQIE010000109        10895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3008_length_10895_cov_7.734594, whole genome shotgun sequence.
ACCESSION   DAOQIE010000109 DAOQIE010000000
VERSION     DAOQIE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3008_length_10895_cov_7.734594"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PK144_08475"
     CDS             complement(<1..176)
                     /locus_tag="PK144_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HNF65800.1"
                     /translation="MDDALWKLCLARLEHELSEQQLNTWVRPLQAIVEDGGLRLLAPN
                     KFVLDHVRENFLDV"
     gene            415..549
                     /gene="rpmH"
                     /locus_tag="PK144_08480"
     CDS             415..549
                     /gene="rpmH"
                     /locus_tag="PK144_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNF65801.1"
                     /translation="MKRTFQPSVLHRKRTHGFRARMATKNGRLVLKARRAKGRARLTP
                     "
     gene            555..917
                     /gene="rnpA"
                     /locus_tag="PK144_08485"
     CDS             555..917
                     /gene="rnpA"
                     /locus_tag="PK144_08485"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNF65802.1"
                     /translation="MAGGARFERRARLVRKADFQAVFDRAEKSGDRYFTVLARPNDLG
                     HARLGLAISKRASKLAVVRNTIKRLVRESFRQRLAGLGGLDFVVMARPGLAEVEHPAL
                     FEALDRHWSRLVKRCARS"
     gene            902..1129
                     /gene="yidD"
                     /locus_tag="PK144_08490"
     CDS             902..1129
                     /gene="yidD"
                     /locus_tag="PK144_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNF65803.1"
                     /translation="MRTLVMLLIRAYRLLLSPLLGPRCRFHPSCSAYALEAVERFGTL
                     RGGWLALRRLGRCHPWHPGGYDPVPPSLERD"
     gene            1129..2799
                     /gene="yidC"
                     /locus_tag="PK144_08495"
     CDS             1129..2799
                     /gene="yidC"
                     /locus_tag="PK144_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNF65804.1"
                     /translation="MENQRLVLVIALAFTAFLIWQQWITEHAPKPAPAAVATTAPATP
                     GSAEAGRSDVPLAPGTPAATAQNSALARGARIKVTTDVYEAEIDSVGGDLRRIGLRRY
                     PESLEQREQPFVLMRDAGGEVFVAQSGLRAAKGAAPDHYAVYTAERSDYALTEGQDSL
                     VVRLDWTDASGVKVVKTYTFHRGSYVIDVAETVENGSAEDWQGVAYWQLQREAPASSG
                     GGIGQTYTYTGGIVSTPEKTYEKHSFDDLKKTPVNETVTGGWVAMIQHYFLGALIPAS
                     QSERNVFYSLAVGGNRYVLGMSGAQLASVAPGASSTFQSKLYVGPKIQDRMAEVAPHL
                     ELTVDYGKLTILAEPLFWALKHLHALLGNWGWAIIVLTLLLKAAFYKLSETSYRSMAN
                     MRKLGPKLQSLKERYGDDKQKLNQAMMEMYRKEKINPLGGCLPIVVQIPVFIALYWML
                     LESVELRQAPFMGWIQDLSVKDPYYVLPLIMGVSMFVQQKLSPAPPDPVQAKVMMAMP
                     VVFTFMFLWFPSGLVLYWVVNNCVSILQQWVITKRIEAGEDKAAATSG"
     gene            2909..4309
                     /gene="mnmE"
                     /locus_tag="PK144_08500"
     CDS             2909..4309
                     /gene="mnmE"
                     /locus_tag="PK144_08500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNF65805.1"
                     /translation="MHESADPAAAPETRVTATPDTTDTIAAIATAPGRGGVGIVRISG
                     PAVPGIAEGLIARLPPPRQAVCRHFRAADGTPIDAGLALYFPAPHSYTGEAVLELQGH
                     GGPVVLDLLLARACALGARPARPGEFTERAFLNGRMDLAQAEAVADLIDAASAQAARA
                     ALASLDGVFSQRVHALADGLLELRLWVEAAIDFPEEDIDFLADGALLARADALASGLD
                     ALLAAAVQGRLLSEGMRVVIAGRPNAGKSSLLNRLAGYEAAIVTDIPGTTRDVLRERI
                     ALDGLPLHVIDTAGLRDSDDPVEQEGIRRAWQEIGRADRLLLLVEDAEGLTAADAALR
                     ARLPATLALTLVRNKIDLSGAAPGEAQGEWGPEVRLSARTGAGLDALAAHLKHCMGFA
                     DKQEGAFTARRRHLDALERTRVHLQDARLRLAGRQGELAAEDLRRAHAALGEITGEVS
                     ADDLLGAIFGQFCIGK"
     gene            4555..5574
                     /locus_tag="PK144_08505"
     CDS             4555..5574
                     /locus_tag="PK144_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65806.1"
                     /translation="MHRSWFSRLFGGSTRDEAFAPVPATEARKAEAGGFDSAESLRAC
                     LDCLPGNVFFADPAFRLTYMNDAARHTVQAVEPEIRRVFGVSAADILNGSIHRFHRDP
                     ARVEAILQNYVQTLPREATFSFGSVTLRTAINAVLTPDRRLLGYIVNWEDVSRLRSME
                     AEVEERRERARQSAEDLLHRLNELRTSVQEISHSAAQVVEVSNTGQTTADAASESIRK
                     LGTQSQEIGSVLKLIDTIAAQTRLLALNATIEAARAGDAGKGFAVVANEVKELAAATA
                     GATEEISAKIGAIRSGVTEAVEAIERIQSIMREVHRLQMTVAAAVEEQSVVVGELGRG
                     ASEVL"
     gene            5712..6083
                     /locus_tag="PK144_08510"
     CDS             5712..6083
                     /locus_tag="PK144_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65807.1"
                     /translation="MGKRALVIDDSRAMRRVIANILTERGFEVLEAENGQVALDQLEA
                     NPGIEVALVDWNMPVMNGYDFIKAVRAQPKYARLTMMMVTTETEMSQVVRALAAGANE
                     YVMKPFTADALVEKLELLGLL"
     gene            6193..7017
                     /locus_tag="PK144_08515"
     CDS             6193..7017
                     /locus_tag="PK144_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNF65808.1"
                     /translation="MTPSSADYDYIRRLVQDHSAIMLEAGKEYLLGLRLQGLMTRHGC
                     ADIPALVQKLRSDRSGALSREVVEAMTNNETSFFRDLHPFETLRKVIVPELIRRRAAE
                     RTLDIWCGASSSGQEIYSVALTLREHFPELVNWRLNIVSTDLSREMVQRGRDGRYSQI
                     EVNRGLPAMLLVKYFERSGMDYYVKPDIRRMVDFRELNLNGPWSGVPSRLDIVFMRNV
                     LIYFEDAVKRRILAKVHDLLRPDGYLFLGGSETTLNLEDRLEQVQLERTRCFRRRH"
     gene            7160..7624
                     /locus_tag="PK144_08520"
     CDS             7160..7624
                     /locus_tag="PK144_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF65809.1"
                     /translation="MQKLPTVLALSTTVLALAVSLSGCEQSDPDSPAFKRRQAFKQIV
                     RAAEPLGKMARGKDPYQPDTFIDRAHELQQVAGLPWDHFPPPQPTDREKTRAKPAIWQ
                     QTEKFETERRNFIAAVNGVVAAGDTRSLDRVRPAVEKLENACMSCHKAFRAD"
     gene            7737..9425
                     /gene="argS"
                     /locus_tag="PK144_08525"
     CDS             7737..9425
                     /gene="argS"
                     /locus_tag="PK144_08525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNF65810.1"
                     /translation="MKTQLAELLSQALARLQAEGLLPAGAAPAEPALERTRDRAHGDY
                     ASNVALACAKAAKRKPRELAEALVAALPASALIAKVEIAGPGFINFFLAADAQAAVVR
                     RVLMQGEGYGRSNTGAGRKVMVEYVSANPTGPLHVGHGRAAAIGDTLARLLAATGWEV
                     TREFYYNDAGVQIANLAVSVQARAQGLTPESPDWPADGYRGDYILEVAKAYLAGESVH
                     ADDREVTGKADPDDTDAIRAFAVAYLRREQDLDLKAFGVGFDRYFLESSLYADGLVEA
                     TVARLAETGHSYEADGALWLRSTDFGDDKDRVMRKRDGTFTYFLPDVAYHRTKWERGH
                     VRAITELGADHHGSLARVRAGLRALDIGIPTGWPEYVLHQMVTVMRGGEEVKISKRAG
                     GYVTLRDLIDEVGRDATRFFFLMRKADSHLVFDVDLARQQSLDNPVYYLQYAHARVCS
                     VLRQAGEKGLDYDEAAGFAALARLVEPHEETLLTLLDRYPEVIELAAGSDEPQLLPAY
                     LRELAQAFHAYYNAHAFLVDDAALRNARLALILAVRRVLASGLDLLGVSAPETM"
     gene            9436..10218
                     /locus_tag="PK144_08530"
     CDS             9436..10218
                     /locus_tag="PK144_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF65811.1"
                     /translation="MSVRRDYKPRTAEKKPANRGVRVLGWAVVTLALFGAGAGGWGWW
                     QKHRAAKAEEAAAAAAAAGQAGPAGGKPSLATLKAQDARAVGSSSTAARPGALPELPP
                     SRFSFYHDLQKRQVQIQQDEIGRSGKGPVPEGALNAQTIVATPPAQAPAPAPAPAPKP
                     APAASPPPAAAQQTASATPAASGRGYLVQAGVFSTSAQAERVRASLALLGVAARIEAG
                     TTADGAAVSRVRLGPYKDAAQAKAVTDKLGEAGIKAIAIKLD"
     gene            complement(10234..10770)
                     /locus_tag="PK144_08535"
     CDS             complement(10234..10770)
                     /locus_tag="PK144_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNF65812.1"
                     /translation="MNPRLLPVALAAALALGPALADEAPLPVATQLDLDRYAGRWYEI
                     ARLPMPYEDDCTGDVRAEYARRPDGGIEVINRCRTAAGESIARGSARVLGPGRLQVRF
                     APDWLAWLPWVWADYQVIELDPQYRWALVGQPSRRYLWVLARTPQLDAAVTEGLLARA
                     RELGFDLTELIRTPQDGG"
BASE COUNT         1690 a         3880 c         3745 g         1580 t
ORIGIN      
        1 atcacgtcga ggaagttctc gcgcacatgg tcgagcacga acttgttcgg cgcgagcagg
       61 cgcaggccgc cgtcctcgac gatggcctgc agcgggcgca cccaggtatt cagttgctgt
      121 tcgctcagct cgtgctccag acgggcgaga cagagcttcc agagcgcgtc gtccacgcgg
      181 gactcctccg gcggcaggct cgggcgaaag ccgcggagtc tatgcgttcg gggccgagtt
      241 atccacagtc ggtccccgct tcgtccacgc ttcctgcacc ggttcttgca ttgacagtgc
      301 cggggccgga ccgctaatct cccgcgcctt gtttgcgcgc cgcgtccgcc ccggacgtgc
      361 gccgccagcg acaccccaga aaaatcccac tgaattcaac cggatcgaac cgccatgaag
      421 cgcaccttcc agcccagcgt cctgcaccgc aagcgtaccc acggtttccg tgcccgcatg
      481 gccaccaaga acggtcgcct ggtgctgaag gcccgccgcg ccaagggtcg tgcgcgcctg
      541 acgccgtaag cgccgtggcc ggcggcgcgc gcttcgaacg gcgcgcccgc ctggtgcgca
      601 aggccgactt ccaggcggtc ttcgaccggg cggaaaaatc cggcgaccgt tacttcacgg
      661 tgctggcccg tccgaacgac ctcggccatg cccggctcgg gctcgcgatc tccaagcggg
      721 cctcgaagct cgccgtggtc cgcaacacga tcaagcgcct cgtgcgcgaa agcttccggc
      781 agcgcctcgc cgggctcggt ggcctcgatt tcgtcgtgat ggcccggccc gggctcgccg
      841 aagtcgaaca tcccgccctc ttcgaagcgc tggaccggca ctggagccga ctggtgaagc
      901 gatgcgcacg ctcgtgatgc tgctcatccg cgcctatcgc ctgctgctga gtccgctgct
      961 cggcccccgc tgccgctttc atccgagctg ttccgcgtac gcgctcgaag ccgtcgaacg
     1021 cttcggcacg ctgcgcggcg gctggctggc cctgcggcgc ctcgggcgct gccacccctg
     1081 gcacccgggc ggctacgatc ccgttccccc gagcctcgag agagactgat ggaaaaccag
     1141 agactcgtgt tggtcatcgc gctcgccttc acggccttcc tgatctggca gcagtggatc
     1201 accgagcacg cgccgaaacc ggcaccggca gcggtcgcga ccactgcccc ggccacgccg
     1261 ggcagcgccg aggccgggcg cagcgacgtg ccgcttgcgc cgggcacgcc ggccgccacg
     1321 gcgcagaaca gcgccctggc gcgcggtgcg cgcatcaagg tcacgaccga tgtctacgag
     1381 gccgagatcg acagcgtcgg cggcgacctg cgccgcatcg gcctgcgccg ctatccggaa
     1441 tcgctggagc agcgcgagca gcccttcgtg ctgatgcgcg atgccggcgg cgaggtgttc
     1501 gtcgcgcagt ccggcctgcg ggccgccaag ggggccgcgc ccgatcacta cgccgtctat
     1561 accgccgagc gcagcgacta tgcgctgacc gagggccagg actcgctggt ggtgcggctc
     1621 gactggaccg acgcctccgg cgtgaaggtg gtcaagacct acaccttcca ccgcggcagc
     1681 tatgtcatcg atgtcgccga gaccgtcgaa aacggctccg ccgaggactg gcagggcgtg
     1741 gcctactggc agctgcagcg cgaggcgccg gcgagcagcg gcggcggcat cggccagacc
     1801 tacacctata ccggcggcat cgtctcgacg ccggaaaaga cctacgagaa gcattccttc
     1861 gatgacctga agaagacgcc ggtcaacgag accgtcaccg gcggctgggt cgcgatgatc
     1921 cagcactact tcctcggcgc gctgatcccg gcctcgcagt cggagcgcaa cgtcttctac
     1981 tcgctcgcgg tcggcggcaa ccgctacgtg ctcggcatga gcggcgcgca gcttgcgagc
     2041 gtcgcgccgg gggcgagcag caccttccag tcgaagctct acgtcggccc gaagatccag
     2101 gaccgcatgg ccgaggtcgc gccgcacctg gagctgaccg tcgactacgg caagctgacg
     2161 atcctcgccg agccgctgtt ctgggcgctg aagcacctgc acgcgctgct cggcaactgg
     2221 ggctgggcga tcatcgtgct gacgctgctg ctgaaggcgg cgttctacaa gctctccgag
     2281 accagctacc gctcgatggc caacatgcgc aagctcgggc cgaagctgca gtcgctgaag
     2341 gaacgctacg gcgacgacaa gcagaagctc aatcaggcga tgatggagat gtaccgcaag
     2401 gagaagatca acccgctcgg cggctgcctg ccgatcgtcg tgcagatccc ggtgttcatc
     2461 gcgctgtact ggatgctgct cgaaagcgtc gagctgcggc aggcgccgtt catgggctgg
     2521 atccaggacc tctcggtcaa ggacccgtac tacgtgctgc cgctgatcat gggcgtgtcg
     2581 atgttcgtgc agcagaagct gtcgccggcc ccgcccgatc cggtgcaggc caaggtcatg
     2641 atggcgatgc cggtggtctt cacgttcatg ttcctgtggt tcccgtccgg tctggtgctg
     2701 tactgggtgg tcaacaactg cgtgtcgatc ctgcagcagt gggtgatcac gaagcgcatc
     2761 gaggccggcg aggacaaggc cgcggccacg agcggctgag cctttccccg tccatcccgc
     2821 aaggcccgcg aggggcgtgt gacgcagacc gcgtccgcgc ccctcgcgca tttgtgccgt
     2881 ccgccgttcc tgctccgagt ctgccgcgat gcacgaatcc gccgatcccg ctgccgctcc
     2941 cgaaacccgg gtcaccgcca ccccggacac caccgacacg attgccgcga tcgccaccgc
     3001 gccgggccgc ggtggcgtcg gcatcgtgcg catcagcggg ccggcggtgc ccgggatcgc
     3061 cgaagggctg atcgcgcggc tgccgccgcc gcgtcaggcg gtctgccggc atttccgcgc
     3121 cgccgacggc acgccgatcg atgccggtct ggcgctgtac ttcccggcgc cgcattcgta
     3181 caccggcgag gccgtgctcg aactgcaggg ccacggcggg ccggtggtgc tcgacctgct
     3241 gctcgcgcgc gcctgcgcgc tcggcgcgcg cccggcgcgg cccggcgagt tcaccgagcg
     3301 cgccttcctg aacgggcgca tggatctcgc ccaggccgag gcggtcgccg acctgatcga
     3361 cgccgccagc gcccaggccg cgcgcgccgc gctcgcctcg ctcgatggcg tgttctcgca
     3421 gcgcgtgcac gcgctcgccg acgggctgct ggaactgcgg ctgtgggtcg aggccgcgat
     3481 cgactttccg gaggaggaca tcgacttcct cgccgacggg gcgctgctcg cccgcgccga
     3541 tgcgctcgcg agcgggctcg atgcactgct cgccgccgcc gtgcaggggc ggctgctcag
     3601 cgagggcatg cgcgtcgtca tcgccgggcg gccgaatgcc ggcaagtcct cgctgctcaa
     3661 tcgtctggcc ggctacgagg cggcgatcgt caccgacatc ccgggcacga cgcgcgacgt
     3721 gctgcgcgag cgcatcgcgc tcgacggcct gccgctgcac gtcatcgaca ccgccggcct
     3781 gcgcgattcc gacgatccgg tcgagcagga aggcatccgc cgtgcctggc aggagatcgg
     3841 acgcgccgac cggctgctgc tgctggtcga ggacgccgag ggtctgacgg ccgcggatgc
     3901 ggcgctgcgt gcgcggctgc cggccacgct ggcgctgacg ctggtgcgca acaagatcga
     3961 cctgagcggg gctgcgcccg gcgaggctca aggcgagtgg gggccggaag tccggctgag
     4021 cgcgcgtacc ggggccggtc tcgatgcgct cgccgctcac ctaaagcatt gcatgggctt
     4081 tgccgataag caggaaggtg cgttcacggc ccgccgccgc cacctcgacg ccctcgaacg
     4141 gacgcgcgtg catctgcagg atgcgcgcct gcgcctggcc gggcgtcagg gcgagcttgc
     4201 ggccgaggat ctgcgccgcg cccatgccgc gctcggcgag atcaccggcg aggtcagcgc
     4261 cgatgacttg ctcggcgcga tcttcgggca attttgcata ggcaagtaag gcttgccacc
     4321 ggaattgcgc agtgcggcat aaccttgaat tactgtaatt tttcctgaca cggcatcgac
     4381 gccggtttgt gctgcacgca atcatggccc tccccgacgg cagcggatgc cggggcgctg
     4441 caccggtccc cggaagcgtc cgcccgccgc cgccgcagcg gccgccggca gtggcgcagt
     4501 cccttgcaga tgccccgacg atctccctga ccccccgcat tccggagagc gcttatgcac
     4561 cgcagctggt tttcccgcct gttcggcggc tcgacacgtg acgaggcctt tgctcccgtg
     4621 ccggcgacgg aggccaggaa agccgaggcc ggcggcttcg attcggccga gagcctgcgc
     4681 gcctgcctcg actgcctgcc cggcaacgtg ttcttcgccg acccggcctt ccggctcacg
     4741 tacatgaacg acgcggcgcg gcacaccgtg caggcggtcg agcccgagat ccggcgcgtg
     4801 ttcggcgtca gcgccgccga catcctgaac ggctcgatcc accgcttcca ccgcgatccg
     4861 gcgcgcgtcg aggccatcct gcagaactac gtgcagacgc tgccgcgcga ggcgaccttc
     4921 tcgttcggca gcgtcacgct gcgcaccgcg atcaacgccg tgctgacccc ggaccggcgc
     4981 ctgctcggct acatcgtcaa ctgggaggac gtctcgcgcc tgcgctcgat ggaggccgag
     5041 gtcgaggaac ggcgcgagcg cgcccgccag tccgccgaag acctgctgca ccggctcaac
     5101 gagctgcgca ccagtgtgca ggagatctcg cacagcgccg cgcaggtggt cgaagtctcg
     5161 aacaccggcc agaccaccgc cgatgccgcc agtgaatcga tccgcaagct cggcacgcag
     5221 agccaggaga tcggcagcgt gctgaagctg atcgacacca tcgccgcgca gacgcggctg
     5281 ctggcgctga acgccacgat cgaggccgcg cgcgccggcg atgccggcaa gggcttcgcg
     5341 gtggtcgcca acgaggtcaa ggaactggcg gcagcgacgg ccggggccac cgaggaaatt
     5401 tccgcgaaga tcggcgccat ccgcagcggt gtcaccgagg cggtcgaggc gatcgagcgc
     5461 atccagtcga tcatgcgcga ggtgcaccgg ctgcagatga cggtggccgc ggcggtcgag
     5521 gaacagagcg tcgtggtcgg cgagctcggc cgcggcgcga gcgaagtgct gtaagcccgg
     5581 cgcgacggcc ggcgggcgac cggtttcaac agatttcaac tgaatttcag acggcggccc
     5641 ggccgccgat ggtgcattca agaccggcac gcgaagctgc ggatcgccgg aagcattggg
     5701 agaatgaggc gatgggcaag cgtgcactgg tgatcgacga ttcgcgggcg atgcgacgcg
     5761 tgatcgcgaa catcctgacc gagcgcggct tcgaggtgct ggaggccgag aacggccagg
     5821 tcgccctcga ccagctcgaa gcgaatcccg gcatcgaggt cgcgctggtc gactggaaca
     5881 tgccggtcat gaacggctac gacttcatca aggccgtgcg tgcgcagccg aagtacgcgc
     5941 gcctgacgat gatgatggtc acgaccgaaa ccgagatgag ccaggtggtg agggccctgg
     6001 ccgccggcgc gaacgaatac gtcatgaagc cgttcacggc cgatgcgctg gtcgagaagc
     6061 ttgaactgct cggactgctc tgaacctcgc gggaccgcga ccggatcgca cggacaagac
     6121 cgcagcccgg acgagcgtca ggatccgtga cgacgcgggg gcgcacaaga aagctgtacc
     6181 gggagtgagg cgatgacacc gagcagtgcc gattacgact acatccgccg gctggtgcag
     6241 gaccactcgg ccatcatgct ggaggccggc aaggaatacc tgctcggcct gcgcctgcag
     6301 ggcctgatga cccgccacgg ctgcgccgac atccccgcgc tggtgcagaa gctgcgcagt
     6361 gaccgcagcg gcgcgctgtc gcgcgaggtc gtcgaggcga tgaccaacaa cgagacctcg
     6421 ttcttccgcg acctgcatcc gttcgagacg ctgcgcaagg tcatcgtgcc cgagctgatc
     6481 cgccgccgcg ccgccgagcg cacgctcgac atctggtgcg gggcctcgtc gagcggccag
     6541 gagatctact cggtggcgct gacgctgcgc gagcacttcc cggagctggt gaactggcgg
     6601 ctcaacatcg tctcgaccga cctgtcgcgc gagatggtcc agcgcggccg cgacggccgc
     6661 tacagccaga tcgaggtcaa ccgcggcctg ccggcgatgc tgctcgtgaa gtacttcgag
     6721 cgctcgggca tggattacta cgtcaagccc gacatccggc gcatggtcga tttccgcgag
     6781 ctgaacctca acggcccgtg gagcggcgtg ccctcgcgcc tcgacatcgt gttcatgcgc
     6841 aacgtgctga tctacttcga ggatgccgtc aagcgccgca tcctcgccaa ggttcacgac
     6901 ctgctgcgcc cggacggcta cctgttcctc ggcggctccg agaccacgct gaacctcgaa
     6961 gaccgcctcg aacaggtgca gctcgaacgc acgcgctgct tccgccgccg gcactgagcc
     7021 ggcacgggcc ggtgatacgg aaaagcctcg tttaagccgg ccatccaccg gcatctgcta
     7081 gagtccgcgc cttctcgcgc gggcctccgg caacagcagc acgggtgccc gctccgtcgc
     7141 tgcccccgga tgtccgccca tgcagaagct cccgaccgtg ctcgccctga gcaccaccgt
     7201 cctcgcgctc gccgtctcgc tttccggctg cgagcagtcc gatccggaca gcccggcctt
     7261 caagcgccgt caggccttca agcagatcgt ccgggccgcc gaaccgctcg gcaagatggc
     7321 gcgcggcaag gacccgtacc agcccgacac cttcatcgac cgtgcgcacg aactgcagca
     7381 ggtcgccggc ctgccctggg atcactttcc gccgccgcag cccaccgacc gcgagaagac
     7441 gcgcgcgaag ccggcgatct ggcagcagac cgaaaaattc gagaccgagc gccgcaactt
     7501 catcgccgcg gtcaacggcg tggtcgccgc cggcgacacg cgcagcctcg atcgcgtgcg
     7561 cccggcggtc gagaagctcg aaaacgcctg catgagctgc cacaaggcct tccgcgccga
     7621 ctgagcctgc agcgctgccg tgctgcgggt gcaacacgcc gccttcaagt cgcggggggg
     7681 cgtggctaca ctgggcgccc tttttccgcc cgccgtcccg cccgaggctt cgcgctttga
     7741 agacgcaact cgccgaactc ctgtcgcagg cgctcgcccg cctgcaagcc gaaggtctgc
     7801 tgccggccgg cgccgctccg gccgaaccgg cgctcgaacg cacgcgcgac cgtgcgcacg
     7861 gcgactacgc gagcaacgtc gcgctggcct gcgcgaaggc ggcgaagcgc aagccgcgcg
     7921 agctggccga ggcgctggtc gcggcgctgc cggcctcggc gctcattgcg aaggtcgaga
     7981 tcgccggtcc cggcttcatc aacttcttcc tcgccgccga tgcgcaggcc gcggtcgtgc
     8041 gccgcgtgct gatgcagggc gaaggctacg gtcgctcgaa caccggcgcc gggcgcaagg
     8101 tcatggtcga gtacgtgtcg gccaacccga ccggcccgct gcacgtcggc cacggccgcg
     8161 ccgcggcgat cggcgacacg ctggcgcggc tgctggcggc gaccggctgg gaggtcacgc
     8221 gcgagttcta ttacaacgat gccggcgtgc agatcgccaa cctcgccgtc tcggtgcagg
     8281 cgcgcgcgca ggggctgacg ccggaatccc cggactggcc ggccgacggc taccgcggcg
     8341 actacatcct ggaggtcgcg aaggcgtatc tcgccggtga gtccgtgcac gccgatgacc
     8401 gcgaggtcac cggcaaggct gatccggacg ataccgacgc gatccgggcc ttcgcggtcg
     8461 cgtatctgag gcgcgagcag gatctcgatc tgaaggcctt cggcgtcggc ttcgaccgct
     8521 acttcctcga atccagcctg tacgccgacg ggctggtcga ggcgaccgtc gcgcgcctgg
     8581 ccgagaccgg ccacagctac gaggccgacg gcgcgctgtg gctgcgctcg accgacttcg
     8641 gcgacgacaa ggatcgcgtg atgcgcaagc gcgacggcac gttcacgtac ttcctgcccg
     8701 atgtcgccta ccaccgcacc aagtgggagc gcggccacgt gcgcgcgatc accgagctcg
     8761 gcgccgacca ccacggctcg ctcgcgcgcg tgcgcgccgg cctgcgggcg ctcgacatcg
     8821 gcatcccgac cggctggccc gagtacgtgc tgcaccagat ggtcaccgtg atgcgcggcg
     8881 gcgaggaggt gaagatctcc aagcgcgccg gcggttacgt gacgctgcgc gatctgatcg
     8941 acgaggtcgg acgcgatgcg acgcggtttt tcttcctgat gcgcaaggcc gattcgcatc
     9001 tggtgttcga tgtcgatctg gcccgccagc agtcgctcga caacccggtc tactacctgc
     9061 agtacgcgca tgcgcgcgtc tgcagcgtgc tgcgtcaggc gggcgagaag gggctcgact
     9121 acgacgaggc ggccgggttc gcggcgctgg cgcggctggt ggagccgcac gaggaaaccc
     9181 tgctgacgct gctcgaccgc tatccggaag tcatcgaact tgcagccggc agcgacgagc
     9241 cgcagctgct gccggcctat ctgcgcgaac tcgcgcaggc cttccatgcc tactacaacg
     9301 cgcacgcctt cctggtcgat gacgcggcgc tgcgcaacgc ccgcctcgcc ctgatcctcg
     9361 cggtgcgccg cgtgctcgcc agcggcctcg acctgctcgg cgtttcggcg ccggagacca
     9421 tgtgagccat agccgatgag cgtgcgccgc gactacaagc ccaggacggc tgaaaagaag
     9481 cctgccaacc gcggcgtgcg cgtgctcggc tgggccgtcg tcacgctggc gctgttcggg
     9541 gccggagccg gaggctgggg ctggtggcag aagcacaggg cggcaaaagc cgaggaggcg
     9601 gccgccgcag cggcagcggc cggtcaggcc ggcccggccg gcggcaagcc gtcgctggcg
     9661 acgctgaagg cgcaggatgc gcgcgcggtc ggcagctcct cgaccgccgc ccggcccggc
     9721 gcgctgcccg agctgccgcc gtcgcgcttt tcgttctatc acgacctgca gaagcgtcag
     9781 gtgcagatcc agcaggacga gatcggccgc agcggcaagg gtccggtgcc cgaaggcgcg
     9841 ctgaacgcgc agaccatcgt cgccactccg ccggcgcaag ctccggctcc ggctccggct
     9901 ccggcgccga aaccggctcc ggccgccagc ccgccgccgg ccgccgcgca gcagacggcg
     9961 agcgccacgc cggccgccag cggtcgcggc tatctggtgc aggccggcgt gttctcgacc
    10021 agcgcgcagg ccgagcgcgt gcgcgcgagc ctcgcgctgc tcggcgttgc agcgcgcatc
    10081 gaggccggca cgaccgccga tggcgccgcc gtcagccgcg tgcgcctcgg gccgtacaag
    10141 gacgccgccc aggccaaggc cgtcaccgac aagctcggcg aggccggcat caaggcgatc
    10201 gcgatcaagc tcgactgacg ggcagggcgg atttcagccg ccgtcctgcg gcgtgcggat
    10261 cagctccgtc agatcgaagc cgagttcgcg cgcccgcgcg agcaggcctt ccgtgaccgc
    10321 ggcatcgagc tgcggcgtgc gcgccagcac ccacaggtag cggcgcgaag gctggccgac
    10381 cagcgcccag cggtactgcg ggtcgagttc gatgacctgg taatcggccc agacccaggg
    10441 cagccacgcg agccagtccg gtgcgaagcg cacctgcagc cggccggggc cgagcacgcg
    10501 ggcgctgccg cgggcgatcg attcgccggc ggccgtgcgg cagcggttga tgacttcgat
    10561 gccgccatcc gggcggcggg catattcggc gcgcacgtcg ccggtgcagt cgtcctcgta
    10621 cggcatcggc agccgggcaa tctcgtacca gcgcccggcg tagcgatcga ggtcgagctg
    10681 cgtggccacc ggcagcgggg cctcgtcggc aagggcaggg cccagggcca gggcggcggc
    10741 gagcgcgaca ggcagcaggc ggggattcat cggggcggct ccggggtttt gcgggtgccg
    10801 gcagccgacc gccgccggcg ccgccggttc ccgctgcctc aggttgatca cgcaagcctg
    10861 cgtctttcag tccttcgccc ctcccgggag acagc
//
LOCUS       DAOQIE010000110        10886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3010_length_10886_cov_7.754409, whole genome shotgun sequence.
ACCESSION   DAOQIE010000110 DAOQIE010000000
VERSION     DAOQIE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3010_length_10886_cov_7.754409"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK144_08540"
     CDS             complement(<1..231)
                     /locus_tag="PK144_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNF65813.1"
                     /translation="MSVEVRVPNLPESVADATLVNWHKKPGDAVKRDENLVDLETDKV
                     VLEVPAPTDGVLGEILQADGSTVKAGDLIATIV"
     gene            complement(270..3128)
                     /locus_tag="PK144_08545"
     CDS             complement(270..3128)
                     /locus_tag="PK144_08545"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNF65814.1"
                     /translation="MSTSFDQFRQTSVLSGGNAAYIEDLYESFLQDPDSVSENWRAYF
                     LGLRAGGNGAAEHLHGPVREVFAKIGQNPRAIISLLPQLSAGESLNPEAAHKQAGVLR
                     LINAHRTRGHQAATLDPLALRERPAVPDLDLAEHGLSEADLDAGFDVGSLQVGKQQLP
                     LREILELVRRVYTGNIGSEYMHIHTTEEKRWIQHRLEGTGARLDLNAAEKKELLDWLV
                     AAEGLEKYLHTKYVGQKRFSLEGGDSLIPMMDALIQHAGASGAKEIVIGMAHRGRLNM
                     LVNILGKAPAELFKEFEGKKKTSAEISSGDVKYHMGFSSDVATPGGVVHLALAFNPSH
                     LEIVNPVVEGSVRARLERRKSGGQDSPLPFLEVLPVLIHGDAAFAGQGVNQELFQMAS
                     VRGYRTGGTVHIVVNNQIGFTTSNPLDVRSSTYCTDVAKIIQAPVFHVNGDDPEAVLF
                     VTKLAMDYRTTFGKDVVIDLVCYRRHGHNEADEPSATQPMMYKKIKARSTAATLYAEQ
                     LVREGVLADGEFTQRVADYRAKLDAGIQVSRPTLEGVSNPYAVDWAPYKNEDWSQPGE
                     TGFPMERLQQLAEKLQTIPDGHEPHARVAKIVDDRRKMAAGALALDWGFAENMAYATL
                     LEEGFQVRVSGEDCGRGTFFHRHAVLHSVVDGSAYIPLANVRPDQPTFTVIDSILSEE
                     AVLGFEYGYATAEPNGLIIWEAQFGDFANGAQVVIDQFISSGQTKWGRLCGLVMLLPH
                     GFEGQGPEHSSARLERYLQLCAEHNMQVIVPSTPAQCFHMLRRQCKRSFRKPAIVMSP
                     KSLLRHRLAVNTLEDLANGRFQEIIPEIDALDPAKVTRIVFCSGKVYYDLLEKRREQN
                     LENVAVVRIEQLYPFPKADYDTEIARYPNATDIVWCQEEPQNQGAWYQSRHHLEAGLS
                     GKHTLSYAGRAASASPAVGSHQVHVEQQTALVSQALGL"
     gene            complement(3453..3860)
                     /locus_tag="PK144_08550"
     CDS             complement(3453..3860)
                     /locus_tag="PK144_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNF65815.1"
                     /translation="MGMQPTPYERLGGAEKIRGLVDRFYELMDTLPEAAAIRRLHPDD
                     LSGSREKLFQYLSGWLGGPPLYESRYGHPRLRARHLPFPIDSGGRDQWLLCMNRALDE
                     SGADPALLDSLRKALAQVADHMRNRPDHDPPVA"
     gene            complement(4092..5756)
                     /gene="ettA"
                     /locus_tag="PK144_08555"
     CDS             complement(4092..5756)
                     /gene="ettA"
                     /locus_tag="PK144_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNF65816.1"
                     /translation="MAQYIYTMNRVSKVVPPKREILKNISLSFFPGAKIGVLGLNGSG
                     KSSLLRIMAGIDTDIHGEARPQAGIKVGYLAQEPQLDAGKDVRGNLETAVQPIKDALA
                     RLDAVYAAYAEPDADFDKLAAEQAELEAYLQATDGHDLERTLEIAADALRLPPWDADV
                     TRLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVAWLERFLHEYPGTVVAVTHD
                     RYFLDNVAGWILELDRGHGIPWEGNYSSWLEQKEQRLEREEKQENARIKAMKQELEWV
                     RSNPKGRHAKSKARLARFEELESQEFQKRNETQELYIPPGPRLGELVVEAENLKKGFG
                     ERLLIDGLNFRLPRGGIVGVIGPNGAGKSTLFRMICAQETPDSGSLRVGDTVQLAYVD
                     QSRDALTGSNTVWQEISDGQDLIQIGSYQINSRAYCGRFNFKGTDQQKFIKDLSGGER
                     NRVHLAKLLRSGGNVLLLDEPTNDLDVETLRALEEALLAFAGCAVVISHDRWFLDRIA
                     THILAFEGDSQVVWFEGNYADYEADRKRRLGVEADQPHRIRYRRLA"
     gene            complement(5928..6200)
                     /locus_tag="PK144_08560"
     CDS             complement(5928..6200)
                     /locus_tag="PK144_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65817.1"
                     /translation="MIPRLPTGLLLGCACCLLAGLPALAAGDSVRRDELFGLIPTGTR
                     TGSNPLQRADQQTCDELARRARLAADIGNRAGQQDSQRQQREAGCP"
     gene            complement(6197..6457)
                     /locus_tag="PK144_08565"
     CDS             complement(6197..6457)
                     /locus_tag="PK144_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65818.1"
                     /translation="MTNLAAMLGRCVRRAICPVLAHDWRFRDIAADGRCTAYCRFCGK
                     CEPARLLLRSPPAAAAPPSVLAAAPTAPDVIDLQARQRELAA"
     gene            complement(6606..6857)
                     /locus_tag="PK144_08570"
     CDS             complement(6606..6857)
                     /locus_tag="PK144_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNF65819.1"
                     /translation="MRALLLPLFAALLAAPAVTDAGEIYRWKDRDGAWHYGDRPPPGA
                     DAEWYDPRPRAGVDRQATPPAAGGSPGAPPAPKPSSGPR"
     gene            6999..8678
                     /locus_tag="PK144_08575"
     CDS             6999..8678
                     /locus_tag="PK144_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNF65820.1"
                     /translation="MEPIWLAEYPPGVPATIDPDSTGTLPELFAESCARFADRAACRS
                     LGHEISYAELARRAGEFTAWLQGLGVAPGTRVALMMPNVLQYPVALFGTLAAGCTVVG
                     VNPLYTPRELEHQLADSGAEVIVILENFAHVLQKVVARTAIRHIVVTAMGDLLPVPRR
                     WLVNAVVRHVKKLVRPYELPRAIPFADTQAGGTPQPVAVAATDVAFLQYTGGTTGLSK
                     GAMLTHRNLAANLLQATAWITPALGGVGNGTIVTALPLYHIFSLTANCLTFLRMGWCN
                     LLIPNPRDIPAFVDELAKAPVHGITGVNTLFNALLNHAEFGRIDWSQLKVSLAGGMAL
                     QRAVAERWAKATGVTLVEAYGLTETSPAVCVNPLSLAEYNAAIGLPLPSTEISIRDAD
                     GVELGIDEAGELCVRGPQVMAGYWQRPDETVKVLDADGWLRTGDIARVDARGYVHLVD
                     RKKDMILVSGFNVYPNEVEDYVMQHPGVLEAAAVGAEDPVCGEVVRLVVVRRDPQLTA
                     EALIAHCRTGLTGYKIPKFVEFRDELPKTPVGKILRRKLRDGDDAGDEGAA"
     gene            8787..10373
                     /locus_tag="PK144_08580"
     CDS             8787..10373
                     /locus_tag="PK144_08580"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNF65821.1"
                     /translation="MSPIAPNSDPRPEALPEKLIVFDTTLRDGEQSPGASMTRDEKIR
                     IAKMLERMRVDVIEAGFPMASPGDFESVQAVARAVRESTVAGLCRATAADIDRCGEAL
                     KDAAQARIHTFIATSPIHMKMKLRMEPDAVIEAAVAAVQRARQWTDDVEFSAEDAGRS
                     EIDFLCRIVEAVIDAGARTINIPDTVGYNVPQQFAETIRALRARVPNAGRAVFSVHCH
                     NDLGLAVANSLAAVLAGARQVECTINGLGERAGNAALEEIVMAVRTRQDVFGCDTRID
                     TTQIVPASRLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVG
                     WTANRMVLGKHSGRNAFRARMEELGFGFGSDAELNTAFARFKDLADKKHEIFDEDLQA
                     LVTEAALEAGNERVRLAYLRVGSETGEIPVAEVVLSVDGSERRARAQGSGPVDAAFKA
                     IEQILEAGAELKLYSVNAITGGTDAQGEVTVRVEKAGRVVNGQGADTDIVIASAKAYV
                     NALGKLGANAGRAHPQLGDV"
     gene            10390..>10886
                     /locus_tag="PK144_08585"
     CDS             10390..>10886
                     /locus_tag="PK144_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65822.1"
                     /translation="MSDRHRRYLAALGIPLWLSRRVLPGAAQPVPADAQALSGPPFEP
                     DPAAAQQAPEGPPALPAGVETRRAAPAGDEAQRLQLPDADADAAAVEPLPAPPQARPA
                     SPARPALAAEAIEPPPAPAVAEPAPALRPVRPPPATPVPADIDEWLPPETDPPPSAPP
                     RPRAQA"
BASE COUNT         1601 a         3696 c         3842 g         1747 t
ORIGIN      
        1 aacgatggtc gcgatcaggt cgccggcctt cacggtcgag ccgtcggcct gcaggatctc
       61 gccgagcacg ccgtcggtcg gcgccggcac ttcgagcacg accttgtcgg tttcgagatc
      121 gacgaggttc tcgtcgcgct tgaccgcatc acccggcttc ttgtgccagt tgaccagcgt
      181 ggcgtcggcg acggactcgg ggaggttcgg aactcggact tctacgctca ttctcgttat
      241 tccctgttga tctggttatg ggccggggct cagaggccga gggcctggct gacgagagcg
      301 gtctgttgtt cgacgtggac ctggtgcgag ccgacggccg gcgaggccga ggcggcacgg
      361 ccggcgtagc tgagcgtgtg cttgccgctc aggccggctt cgaggtgatg gcggctctgg
      421 taccacgcac cctggttctg cggctcttcc tggcaccaga cgatgtcggt ggcattcgga
      481 tagcgcgcga tctcggtgtc gtagtcggcc ttcgggaacg ggtagagctg ctcgatgcgc
      541 acgaccgcga cgttctcgag gttctgctcg cgacgctttt cgagcaggtc gtagtagacc
      601 ttgcccgagc agaacacgat gcgcgtgacc ttggccggat cgagggcgtc gatctccggg
      661 atgatctcct ggaagcggcc gttggcgagg tcttcgagcg tgttcaccgc caggcggtga
      721 cgcagcaggc tcttcgggct catgacgatc gccggcttgc ggaagctgcg cttgcactgg
      781 cgacgcagca tgtggaagca ctgcgccggc gtcgacggca cgatgacctg catgttgtgc
      841 tcggcgcaca gctgcaggta gcgctccaga cgcgccgagg aatgctccgg accctgaccc
      901 tcgaagccgt gcggcagcag catcaccagg ccgcagagcc ggccccactt ggtctggccc
      961 gaggagatga actggtcgat gaccacctgc gcgccgttgg cgaagtcacc gaactgcgct
     1021 tcccagatga tcaggccgtt cggctcggcg gtcgcgtagc cgtactcgaa gccgagcacg
     1081 gcctcttcgg acaggatcga gtcgatgacc gtgaaggtcg gctggtcggg cctgacgttg
     1141 gcgagcggga tgtaggccga gccgtcgacg acgctgtgca gcaccgcatg gcggtggaag
     1201 aacgtgccgc gaccgcagtc ctcgccggag acgcgcacct ggaagccttc ttcgagcagc
     1261 gtcgcgtagg ccatgttctc ggcgaagccc cagtcgaggg ccagcgcacc ggcggccatc
     1321 ttgcggcggt catcgacgat cttggcgacg cgggcgtgcg gctcgtggcc atcggggatg
     1381 gtctgcagct tctcggcgag ctgctgcagg cgctccatcg ggaagccggt ctcgccgggc
     1441 tgcgaccagt cctcgttctt gtacggtgcc cagtcgaccg cgtacgggtt cgagacgcct
     1501 tcgagcgtcg ggcgcgagac ctggatgccg gcgtcgagct tggcgcggta gtcggcgacg
     1561 cgctgcgtga actcgccgtc ggcgagcacg ccctcgcgca cgagctgctc cgcatacagc
     1621 gtcgcggcgg tcgagcgggc cttgatcttc ttgtacatca tcggctgcgt ggccgagggc
     1681 tcgtcggcct cgttgtggcc gtggcggcgg tagcagacca gatcgatgac cacgtccttg
     1741 ccgaaggtcg tgcggtagtc catcgccagc ttggtgacga acagcacggc ctcgggatcg
     1801 tcgccgttca cgtggaacac cggcgcctgg atgatcttgg cgacgtcggt gcagtacgtg
     1861 gacgaacgca cgtcgagcgg gttcgaggtc gtgaagccga tctggttgtt gacgacgatg
     1921 tgcacggtgc cgccggtgcg gtagccgcgc acgctcgcca tctggaacag ctcctggttg
     1981 acgccctggc cggcgaaggc cgcgtcaccg tggatcagca ccggcagcac ttcgaggaac
     2041 ggcagcggcg agtcctggcc gccggacttg cgccgctcca gacgcgcacg caccgagccc
     2101 tcgaccaccg ggttgacgat ttccaggtgc gaggggttga aggcgagcgc cagatgcacg
     2161 acgccgcccg gcgtcgccac gtccgacgag aagcccatgt ggtacttgac gtcgcccgag
     2221 gagatttcgg cgctggtctt cttcttgccc tcgaactcct tgaacagctc cgccggcgcc
     2281 ttgccgagga tgttcacgag catgttcagg cggccgcggt gggccatgcc gatgacgatt
     2341 tccttcgcac cgctcgcgcc ggcgtgctgg atcagcgcgt ccatcatcgg gatcagcgag
     2401 tcgccgcctt cgagcgagaa gcgcttctgg ccgacgtact tggtgtgcag gtacttttcg
     2461 aggccctcgg cagcgaccag ccagtcgagc agttccttct tctcggccgc gttcaggtcg
     2521 agcctcgcgc cggtgccttc gaggcgatgc tggatccagc gcttctcctc ggtggtgtgg
     2581 atgtgcatgt actccgagcc gatgttgccg gtgtacacgc gacgcacgag ctcgagaatc
     2641 tcgcgcagcg gcagctgctg cttgccgacc tgcagcgagc cgacgtcgaa gccggcatcg
     2701 agatcggcct cgctcaggcc gtgctcggcg agatcgaggt ccggcaccgc cgggcgctcg
     2761 cgcagcgcca gcgggtcgag ggtcgcggcc tggtggccgc gcgtgcggtg ggcgttgatc
     2821 aggcgcagca cgccggcctg cttgtgcgcg gcctcgggat tcagcgactc gccggcgctg
     2881 agctgcggca gcagcgagat gatcgcgcgc gggttctggc cgatcttcgc gaacacttcg
     2941 cgcaccggcc cgtgcaggtg ctcggccgcg ccgttgccgc cggcgcgcag gccgaggaaa
     3001 tacgcccgcc agttctcgga aaccgaatcg ggatcctgca ggaagctctc gtagaggtct
     3061 tcgatatacg cagcattgcc gccggacagg accgaagtct gccggaactg atcgaatgag
     3121 gtgctcattt ggcttctgtt gactccccgt cgttgaaaaa ccccgtgcga accgccggac
     3181 agcttcggct cggtcagggt gctcgtaggc ctcgacctgg atgctgattt tttcggtacc
     3241 actcgccgga tccgggaccg gcctgccgtc ttgctgcggc tcttttcgcc gcggtatgtc
     3301 ccgcggtcgg tttatttatt cgttttcatc gcggcagccg tgccgtcgtt cgttcttccc
     3361 ttcgccggtg ctggcaccgg aggttttgcg cggcagcggc cgcacttcgt gaaatctgga
     3421 tttcgtatcg tgcctcggac gctccggctg gttcaggcca ccggcgggtc gtgatcgggc
     3481 cggttgcgca tgtggtcggc cacctgggcc agcgccttgc gcagcgaatc gagcagcgcc
     3541 ggatcggcgc cgctttcatc gagtgcacgg ttcatgcaga gcagccactg gtcgcggccg
     3601 ccggagtcga tcgggaacgg caggtgacgc gcgcgcaggc gcggatgtcc gtagcgggac
     3661 tcgtacagcg gcggaccgcc gagccagccg gacaggtact ggaagagctt ttcccgcgat
     3721 ccggaaaggt cgtccggatg caggcggcgg attgccgcgg cttcgggcag cgtgtccatc
     3781 aactcgtaga aacggtcgac gagcccgcgg attttctccg cgccgccgag tcgttcataa
     3841 ggagttggct gcatgcccat cgtgaaggag tttcgcgctg ccctgagcca cgcttgcgcc
     3901 cggttccggt tcggtccggg tctggaagtc ggcggattct aaaccttgcg gcacacgact
     3961 tgaagctgca aaacctcgct gggcgagtgc attcgtttcg gatgacgaag cgctccggag
     4021 gcttgcgccg gactgcgaag aagcgtgcgg cggtcgcagc atgaaggcga gcgccgcacg
     4081 gggcggggat ttcaggccag gcgcctgtag cggatgcggt gcggctggtc agcctcgacg
     4141 ccgaggcggc gcttgcggtc ggcctcgtag tcggcgtagt tgccctcgaa ccagaccacc
     4201 tgcgaatcgc cctcgaaggc gaggatgtgc gtggcgatgc ggtcaaggaa ccagcgatcg
     4261 tgcgagatca ccaccgcgca gccggcgaag gcgagcaggg cttcctccag cgcgcgcagc
     4321 gtctcgacat cgaggtcgtt ggtcggctcg tcgagcagca gcacgttgcc gccgctccgg
     4381 agcagcttcg cgaggtgcac gcggttgcgc tcgccgcccg agaggtcctt gatgaacttc
     4441 tgctggtccg tgcccttgaa gttgaagcgc ccgcagtagg cgcgcgagtt gatctggtag
     4501 ctgccgatct ggatgagatc ctgaccgtcg gagatttcct gccagaccgt gttgctgccg
     4561 gtcagcgcgt cgcggctctg gtcgacgtag gcgagctgca ccgtgtcgcc gacgcgcagg
     4621 ctgccgctgt ccggggtttc ctgggcgcag atcatccgga acagcgtcga cttgccggcg
     4681 ccgttcggac cgatgacgcc gacgatgccg cccctgggca gacggaagtt caggccgtcg
     4741 atcagcaggc gctcgccgaa gcccttcttc aggttctcgg cctcgaccac cagctcgccg
     4801 agccgcggcc ccggcgggat gtagagctcc tgggtctcgt tgcgcttctg gaattcctgc
     4861 gattcgagtt cctcgaagcg cgccagacgc gccttggact tggcgtggcg gcccttgggg
     4921 ttgctgcgca cccattcgag ctcctgcttc atcgccttga tgcgcgcgtt ctcctgcttc
     4981 tcctcgcgct ccaggcgctg ctccttctgc tccagccacg aggagtagtt gccctcccac
     5041 gggatgccgt gcccgcggtc gagttcgagg atccagccgg cgacgttgtc gaggaagtag
     5101 cgatcgtgcg tgaccgcgac cacggtgccc ggatactcgt gcaggaagcg ttcgagccag
     5161 gcgaccgatt cggcgtcgag gtggttggtc ggctcatcga gcagcagcat gtcgggcttc
     5221 gacagcagca gccggcagag cgcgacgcgg cgcttctcgc cgcccgagag cctggtgaca
     5281 tcggcgtccc acggcggcag gcgcagcgcg tcggcggcga tctccagcgt gcgctccaga
     5341 tcgtggccgt cggtggcctg cagataggct tcgagttcgg cctgctcggc ggcgagcttg
     5401 tcgaagtcgg cgtcgggttc ggcataggcc gcgtagacgg catcgaggcg cgcgagcgcg
     5461 tccttgatcg gctgcacggc ggtctcgagg ttgccgcgca cgtccttgcc ggcatcgagc
     5521 tgcggctcct gcgccagata gccgaccttg atgccggcct gcggccgggc ctcgccgtgg
     5581 atgtcggtgt cgatgccggc catgatccgc agcagcgagg acttgcccga gccgttgagg
     5641 ccgagcacgc cgatcttggc gcccgggaag aacgacagcg agatgttctt caggatctcg
     5701 cgtttcggcg gcaccacctt cgaaacgcgg ttcatcgtgt agatgtactg agccatgcgg
     5761 agtcccaggg ggtcgaggta cgcgcgaatg ccgggcgcgc cgcagcggtc acggtcgacg
     5821 ggcgtccggc agcgggatca tcgcgacggg cagtgcggca ccgccggcga ggcgccgaac
     5881 gatacaccgc attgccggcc ccggcacggg gccggggcag gcgccggcta cgggcagccg
     5941 gcctcgcgct gctggcgctg ggaatcctgc tggccggcgc ggttgccgat gtcggccgcg
     6001 agccgggcgc ggcgggcgag ttcgtcacag gtctgctggt cggcccgctg cagggggttc
     6061 gagccggtgc gcgtaccggt cgggatcagg ccgaacagct cgtcccggcg gacggaatcg
     6121 ccggcggcaa gcgccggcag gccggcgagc aggcagcagg cgcagccgag cagcaggccg
     6181 gtcggcagcc gggggatcat gcggcgagtt cccgttgccg ggcctggagg tcgatgacat
     6241 ccggagcggt cggggccgcc gccagcactg aaggcggtgc ggcggccgcg ggcgggctgc
     6301 gcagcagcag ccgtgccggt tcgcacttgc cgcagaagcg gcagtacgcg gtgcagcgtc
     6361 cgtcggccgc gatgtcgcgg aaacgccagt cgtgggccag caccgggcag atcgcgcgac
     6421 gcacgcagcg gccgagcatg gcggcaagat tcgtcatggc gacgggcctc tgcaacgggg
     6481 ggagggggcg cgtccttgtg ctggtgcagc atcaggtctt gcgaggctag cggcgcgatt
     6541 gtgcaggtgc aagaaattcg caaagcgatg gattgcgcag tgcggcaaaa cacgggctgg
     6601 cggtctcagc gcgggccgga ggacggtttc ggggccgggg gcgcgcccgg cgagccgccg
     6661 gccgccggcg gcgtcgcctg acggtcgacg ccggcacgcg ggcgcgggtc gtaccactcg
     6721 gcgtcggcgc ccggaggcgg gcggtcgccg tagtgccagg caccgtcgcg atccttccag
     6781 cggtagatct cgccggcatc ggtgacagcc ggggcggcga gcagggcggc gaacagcggc
     6841 aggaggagcg cgcgcatgac ggtctccggg cgtggcgatg actccgagtc tagtccgcgc
     6901 cggcaggaag ccgcgggggc ggccgtatac gcttgccggg atggctgcca acaccgatgc
     6961 aggcgccatc ctcttccgca accgcacggg agccccccat ggaaccgatc tggctggccg
     7021 aatacccccc cggcgtgccg gcgacgattg accccgacag caccggcacg ctgccggaac
     7081 tgttcgccga atcctgcgca cgctttgctg accgggcagc ctgtcgcagc ctcgggcacg
     7141 agatcagcta cgccgaactc gcgcgccgtg ccggcgagtt caccgcctgg ctgcagggcc
     7201 tcggcgtggc gccgggcacg cgcgtcgcgc tgatgatgcc gaacgtgctg cagtacccgg
     7261 tggcgctgtt cggcacgctc gccgccggct gcacggtggt cggcgtcaat ccgctgtaca
     7321 cgccgcgcga gctcgaacac cagctcgcgg attccggtgc cgaggtcatc gtgatcctcg
     7381 agaacttcgc gcacgtgctg cagaaggtcg tcgcgcgcac ggcgatccgg cacatcgtgg
     7441 tcacggcgat gggcgacctg ctgccggtgc cgcggcgctg gctggtcaac gccgtcgtgc
     7501 gccacgtgaa gaagctggtg cgtccgtacg agctgccgcg ggcgatcccg ttcgccgaca
     7561 cccaggccgg cggcacgccg cagccggtgg cggtggccgc gaccgacgtg gctttcctgc
     7621 agtacaccgg cggcacgacc gggctgtcca agggcgcgat gctcacgcac cgcaacctcg
     7681 ccgccaacct gctgcaggcg acggcctgga tcacgccggc gctcggcggc gtcggcaacg
     7741 gcaccatcgt cacggcgctg ccgctctatc acatcttctc gctgaccgcg aactgcctga
     7801 ccttcctgcg catgggctgg tgcaacctgc tgatcccgaa cccgcgcgac atcccggcct
     7861 tcgtcgatga gctcgcgaag gcgccggtgc acggcatcac cggcgtcaac acgctgttca
     7921 acgcgctgct gaaccacgcc gagttcgggc gcatcgactg gtcgcagctg aaggtgtcgc
     7981 tcgccggcgg catggcgctg cagcgggcgg tcgccgagcg ctgggcgaag gccaccggcg
     8041 tgacgctggt cgaggcctac ggcctcaccg aaacctcgcc ggccgtgtgc gtgaacccgc
     8101 tgtcgctcgc cgagtacaac gccgcgatcg gcctgccgct gccctcgacc gagatctcga
     8161 tccgcgatgc cgacggcgtc gaactcggca tcgacgaggc cggcgaactc tgcgtgcgcg
     8221 gcccgcaggt gatggccggc tactggcagc ggcccgacga aaccgtgaag gtgctcgatg
     8281 ccgacggctg gctcaggacc ggcgacatcg cccgcgtcga tgcgcgcggc tacgtgcatc
     8341 tcgtcgatcg caagaaggac atgatcctgg tctcgggctt caacgtgtac ccgaacgagg
     8401 tcgaggacta cgtgatgcag caccccggcg tgctcgaagc cgccgcggtc ggcgccgagg
     8461 acccggtctg cggcgaggtg gtcaggctcg tcgtcgtgcg ccgcgatccc cagctcacgg
     8521 ccgaggccct gatcgcgcac tgccgcaccg gcctcaccgg ctacaagatt ccgaaattcg
     8581 tcgaattccg cgacgagctg ccgaagacgc cggtcggcaa gatcctgcgc cgcaagctgc
     8641 gcgacggtga cgacgccggg gacgagggcg cggcctgagt gcacgggtgc gcggcttggc
     8701 agtgccgggg tgcaatccta tagtgccgcg tctgtcctgc ccgttgccgc ctgcccgccg
     8761 tgcgcgcagg cccggagtct gcgccgatga gtccgatcgc cccgaattcc gacccccgcc
     8821 ccgaagccct gcccgagaag ctgatcgtct tcgacacgac gctgcgcgac ggcgagcaga
     8881 gccccggcgc ctcgatgacg cgcgacgaga agatccgcat cgcgaagatg ctcgaacgca
     8941 tgcgcgtcga cgtgatcgag gccggcttcc cgatggcgag ccccggcgac ttcgaatccg
     9001 tgcaggcggt cgcgcgcgcg gtgcgcgagt cgaccgtcgc cggactctgc cgcgccacgg
     9061 ccgccgacat cgaccgctgc ggcgaggcgc tgaaggacgc cgcgcaagcg cgcatccaca
     9121 ccttcatcgc caccagcccg atccacatga agatgaagct gcgcatggag cccgacgccg
     9181 tcatcgaggc ggcggtcgcg gccgtgcagc gcgcgcgcca gtggaccgac gatgtcgagt
     9241 tctcggccga ggatgccggg cgctccgaga tcgacttcct ctgtcgcatc gtcgaggcgg
     9301 tcatcgacgc cggcgcacgc acgatcaaca ttccggacac ggtcggctac aacgtgccgc
     9361 agcagttcgc cgagaccatc cgcgcgctgc gcgcgcgcgt gccgaacgcc ggacgtgcgg
     9421 tgttctcggt gcactgccac aacgacctcg ggctcgcggt cgccaactcg ctggccgccg
     9481 tgctcgccgg cgcccgccag gtcgaatgca cgatcaacgg cctcggcgag cgcgccggca
     9541 acgccgcgct tgaggaaatc gtcatggctg tgcgcacgcg ccaggatgtc ttcggctgcg
     9601 acacgcgcat cgacaccacg cagatcgtgc cggcctcgcg gctggtctcg ggcatcaccg
     9661 gctttccggt gcagccgaac aaggccatcg tcggcgccaa cgccttcgcg cacgaatccg
     9721 gcatccatca ggacggcgtg ctcaagcacc gcgagaccta cgagatcatg cgcgccgagg
     9781 acgtcggctg gaccgccaac cgcatggtgc ttggcaagca ttcggggcgg aacgccttcc
     9841 gtgcgcgcat ggaggaactc ggcttcggct tcggctccga tgccgagctg aacaccgcct
     9901 tcgcgcgctt caaggacctt gccgacaaga agcacgagat cttcgacgag gacctgcagg
     9961 cgctcgtcac cgaggccgcg ctcgaagccg gcaacgagcg cgtgcgtctc gcctatctgc
    10021 gcgtcggctc cgagaccggc gagatcccgg tcgccgaggt cgtgctgagc gtcgacggca
    10081 gcgaacggcg cgcccgcgcc cagggctccg gcccggtcga tgccgcgttc aaggccatcg
    10141 agcagatcct cgaagccggc gccgagctga agctctactc ggtcaacgcc atcaccggcg
    10201 gcaccgacgc ccagggcgag gtcaccgtgc gcgtcgaaaa ggccgggcgc gtcgtcaacg
    10261 gccagggcgc cgacaccgac atcgtcatcg cctcggccaa ggcctacgtc aacgccctcg
    10321 gcaagctggg cgcgaatgcc gggcgcgcgc acccgcagct cggcgacgtc tgagtccgcc
    10381 gtttccggga tgtccgaccg tcaccgccgc tatctcgccg cgctcggcat cccgctgtgg
    10441 ctgagccggc gcgtgctgcc gggcgcagcg cagccggtgc cggcggacgc acaggcgctg
    10501 tccgggccgc ccttcgagcc ggaccccgcc gccgcgcagc aggcgcctga gggcccgccg
    10561 gcgctgcccg ccggcgtgga aacccgcagg gcagcgcctg ccggcgacga ggcgcagcga
    10621 ctgcagcttc cggatgccga tgccgatgcc gctgccgtcg agccgctgcc cgcaccgcca
    10681 caggcccgtc ccgcatcccc cgcgcggccg gccctcgccg ccgaagcgat cgaaccgcct
    10741 cctgcccccg ccgttgcaga gcctgcgccg gcgctgcggc ccgtgcgccc gccgccggcc
    10801 accccggtgc cggccgacat cgacgaatgg ctgccacccg aaaccgatcc gccaccgtcc
    10861 gcaccgccgc gcccgcgcgc gcaagc
//
LOCUS       DAOQIE010000111        10790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3054_length_10790_cov_7.198510, whole genome shotgun sequence.
ACCESSION   DAOQIE010000111 DAOQIE010000000
VERSION     DAOQIE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3054_length_10790_cov_7.198510"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..914)
                     /gene="ugpQ"
                     /locus_tag="PK144_08590"
     CDS             complement(168..914)
                     /gene="ugpQ"
                     /locus_tag="PK144_08590"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNF65823.1"
                     /translation="MSSGRWCWPRVLAHRGAGRFAPENTLAALRKGAALGARALECDV
                     KLSRDGVCVLLHDDTLERTTDGHGRASQHDWATLARLDAGSWHSPEYAGEPPAQLGAI
                     AAACRALDHAINVEIKPEPGLETGTGWSVAHACEQLWAGAALPPLLSSFSVAALHSAR
                     LAAPRLPRALLVEAIPPDWRAQLESLEAVALHCWHGALTPALAAEVKSAGFALFCYTV
                     DEPQRARELFGWGVDGLCTDRPDVVTEAIA"
     gene            complement(911..2473)
                     /locus_tag="PK144_08595"
     CDS             complement(911..2473)
                     /locus_tag="PK144_08595"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNF65824.1"
                     /translation="MPCSPRALRERLRIRLPLLLAPMAAPAGTPALCAAVANAGGLGS
                     LCGAELPPEALRAAICAVRALTDQPFAVHLHAPVPYVIDGDRIEYTRLLLAPYHDALG
                     LAEPPEPMPPPSFEAQLEVLLDEGIEVASFAGGLPPDELLAPLLAAGVTVIGTATHLL
                     EALVLEESGVHIVVAQGAEAGGLRGGFAGAPERVALGTCALVPLLADHLSLPVVAAGG
                     LMDGRGIAAALNLGASGVMLGTAFLGCPETGLSPACRERLLAATELDSINGRLWLGRC
                     ARQLDDRLQRELASEAGNLPVWPVQQQLTTALRHAAAAQDRPELQPLWAGQGIALLRA
                     APAAGLIAAWIDDAGRRLGQPLVQPPELPNPHVDDDGSEAVPAALPDPATGDDAGRDE
                     PDGTEDTEDDPAGDLPDVGDAGSLQVLAAAPADELPEVIWHELPDPADEPAAAPCAPE
                     PAAPEAGLPADAAADWSLPPVATASLDQLAAEAAPCEAERFDEVDRPAPLQLGAAAPA
                     PPATARPLAEVP"
     gene            complement(2535..3353)
                     /locus_tag="PK144_08600"
     CDS             complement(2535..3353)
                     /locus_tag="PK144_08600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNF65825.1"
                     /translation="MNPSALPAAAPQPELVDSHSHFDVGEFDADRDAAYARARAAGVV
                     QQVVPGVIARSWDALDAVVARYPGLYAAWGLHPCYLAEHRPAHLAELRARVAARRPVA
                     IGECGLDAFLPQPDLPAQETYFLEQLRIARDAGLPVIVHARRAVDAVLKCIRRIGGRE
                     GLSGVVHSFAGSIEQAERLREHGFLVSLGGPLSYERAQRLHRLVRALPADGLLLETDA
                     PDQPGVQHRGERNEPAYLPEVLATVAALRGESPAAVAAASTAAARRLFGLPAAA"
     gene            3488..4156
                     /locus_tag="PK144_08605"
     CDS             3488..4156
                     /locus_tag="PK144_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF65826.1"
                     /translation="MRPSFPVLTMFAALAAAGPVAAGVLADFEGLATPPPVDGATGFE
                     FANGGSLDYAGVRWDDRIQVVGDAYRVDTATPGPLFGLPHSGSFFINNHNGADGILLE
                     TTQVLTGAWFARNEYYGFGGGADQVSITALGAGGDLLTLSLDLPELLAGLPEQPGFFD
                     TSAFLALSGITGYRIDRRETGPFSADWVADDFTFEAAVTAIPAPSVLALFGLGGLGLC
                     RRRR"
     gene            4244..4627
                     /locus_tag="PK144_08610"
     CDS             4244..4627
                     /locus_tag="PK144_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65827.1"
                     /translation="MKPHPLTFVLPALLLAACAASDPGAAPARVADGVWVGGNGMTLY
                     VFDKDVAGSGRSACNGACAANWPPLAAAADAPAGGDWTTIRRDDGSRQWAWRGRPLYF
                     WSKDTQPGQRSGDGFNQVWHVARAG"
     gene            complement(4634..6100)
                     /locus_tag="PK144_08615"
     CDS             complement(4634..6100)
                     /locus_tag="PK144_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF65828.1"
                     /translation="MSGSANLPGHFRPRVLIVEDSRAMAEVYMGYLKREAWRLVHVET
                     GAEALAVIEREPPHALLLDLNLPDMNGMEILRAVVARGLPVAVVVITANDSVLLAVQA
                     MRDGAWDYLVKTQFDADRLRLTVRNALERQRLDQLVATLRDGARDGYCGFIGSSLPMQ
                     AVYRIIEAAAPSRATVFITGESGTGKEICAEAIHRQSPRRDGPFIALNCAAIPRDLME
                     SEIFGHVKGAFTGATSERKGAAALADGGTLFLDELCEMPAELQTKLLRFVQTGSFQPV
                     GSGRIEKVDVRFICATNRDPRQEVDEGRFREDLYYRLHVIPLHLPPLREREDDVLAIA
                     RHFLVEYAAEEGRRFQGFRADTEAVLLEYGWPGNVRELQNVVRNIVVLNDAGEVTPQM
                     LPAPLSRVVAAGEAAVTARSESREVPPAAALAAELPPGDPTLRIRPLEQVERELIEDA
                     IAACDGNIPKAAALLEISASTIYRKRQAWADADAAHSA"
     gene            complement(6267..7391)
                     /locus_tag="PK144_08620"
     CDS             complement(6267..7391)
                     /locus_tag="PK144_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF65829.1"
                     /translation="MHDLSPGAARAFADCSAGPSDAPAPRPRVLVLDDDAAQRELIRL
                     YLQDDYDVIGYAAADAALAALAQQRYDLVVSDIHLPGLDGLGFRARLAERNDTDLLPF
                     IFITGTADERLKRDADQLGIDDYLLKPLRKRELLGTLRRVLARGRARRLRWIDAAREQ
                     LERTLRPQLPERLGGCRVVSGQRGSALLDGLYAGRGHDPAEGVSQVILVRLAGDDDEY
                     GSAAQLRASYLAGLLRHLPHGQTPAATLATLLARTSVDPVLAGVRAEALVVHIGPSAQ
                     VSLAGSGRVGAWVLASGGFAQSLELAGPLERRLAPGERLLLMSRAPAPLAGGGSVPGF
                     LAQITARQPLPMAAERLLDLLGADPGLLLLLEPLAPAGRS"
     gene            complement(7525..10476)
                     /locus_tag="PK144_08625"
     CDS             complement(7525..10476)
                     /locus_tag="PK144_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF65830.1"
                     /translation="MKDQPPQRSLSNRLFGFLRAEEPASGAGEPMPATIDAAVIAAAA
                     EAILTLDERGTIVRFNPAAEKLSGYAAAEVIGRNFRVLLPAAVLETLGDDPLRTLTRG
                     LPGATSELGVRRKDGSLASVELSVSAFSAEGRAYFAAIVRDISRRKRAESQLRASEGL
                     KAAMLEAALDAVITIDQDGRLLEFNPAAERIFGYARAEVLGQPMADLLIPGRLRSEHR
                     VGMARYLDTGEATQMIGRRTEVMALRADGSEFPAEIAVTPIQAGPQRLFTAFLRDITE
                     RRQAEEELRIAKDQAESANRAKSEFLATMSHEIRTPMNAVLGTLTLLLDTPLTEEQRN
                     FAETASESGKALLTIINDILDFSKIEAGKLDLESTEFDLVQLIEGVAELLAPRAHGKH
                     IEIATWIGVDLPARVTADAGRLRQILLNLAGNAVKFTRKGGVALHVAREPVARPAGRI
                     RLRFEIQDTGIGIPREAQARLFEKFSQSDPSHSRKYGGTGLGLAISRRLVELMGGEIG
                     FTSAPGLGSTFWFTIDVGCPSGDRLTTPGVIATLGGVRVLIVEDNPVTRRLRERELAS
                     WGMAVEAVANGTAALATLKDARSGGRPFDVALIDQWLADMRGEELGQGILRDPQLEST
                     ALILMATMGTPSIAQRVRRMGFHASLTKPVRQQSLHRWLAVAIGLNDAEEARRREESE
                     VRLTAPEPVPAAPPPRKFGRVLLVEDSQVNQTVAIAMLKKAGYQVDAVGNGLEAVQAV
                     RTLPYDLVLMDLAMPEMDGFEATAEIRHMPGPERDLPIIAMTANALPGDRERCLAAGM
                     DDYLTKPIDRAQLLATLERWLAQPEADAARRAAEAANDAGNSTGPQPVVSAAAAAAPP
                     PPSPLPSPPVAPAVPVVDQAVLDQLEADTDHDVLGRVVQLFVEESRVRLDSIDAALTR
                     DDWKTLQREAHTLKSSAGTFGARQLQEHARQLNDFCRAGQLERARELALSIRAVAVPA
                     LIELDRLFPRR"
BASE COUNT         1378 a         3793 c         4115 g         1504 t
ORIGIN      
        1 tccccggatt catgccggtc acacgtaaac gcatggtttt tctccccctc tccccaaccc
       61 ctctcccacg aggggcgagg ggctgaaaaa gcgaagcgtt ctgatgtgat cggtatccat
      121 ccaccgcgga ttcatcacgg atgcgcatcc ccggcgcacc ggccggttca ggcgattgct
      181 tcggtcacca cgtccggccg atccgtgcaa agcccgtcca cgccccatcc gaacagttcc
      241 cgcgcccgtt gcggttcatc gaccgtgtag cagaacagcg cgaaaccggc cgatttcacc
      301 tctgccgcca gagccggcgt cagtgcgccg tgccagcagt gcagcgccac ggcctccagg
      361 gattcgagct gcgcacgcca gtccggcggg atcgcctcga ccagcagcgc gcgcggcagc
      421 cgcggggcgg cgagccgggc cgagtgcagc gcggcgacgc tgaagctcga cagcagcggc
      481 ggcagcgcag cgcccgccca gagctgctcg caggcgtgcg cgacggacca gccggtgccg
      541 gtctccagac cgggttcggg tttgatctcg acgttgatcg cgtgatcgag cgcgcggcag
      601 gcagcggcga tggcgccgag ctgcgcgggc ggttcgccgg cgtattcggg cgaatgccag
      661 ctgccggcgt cgaggcgggc gagcgtggcc cagtcgtgct gcgaggcgcg gccgtggccg
      721 tcggtggtgc gctccagcgt gtcgtcatgc agcagcacgc agaccccgtc gcggctcagc
      781 ttcacgtcgc attccagtgc gcgcgccccg agtgccgcgc ccttgcgcag cgccgccagc
      841 gtgttttccg gcgcgaagcg cccggcgccg cgatgcgcga gcacgcgcgg ccagcaccag
      901 cggccgctgc tcatggcact tccgccagcg ggcgcgcggt tgctggcggt gcgggcgcgg
      961 cggcgccgag ctgcagcggc gccgggcggt cgacctcgtc gaagcgttcg gcctcgcagg
     1021 gcgcggcctc ggcggcgagc tgatcgaggc ttgcggtcgc gaccggcggc agcgaccagt
     1081 cggcggccgc atcggccggc agcccggcct cgggcgccgc cggctccgga gcgcagggcg
     1141 cggcggcggg ctcatcggcc gggtccggaa gctcgtgcca gatgacttcg ggcagctcgt
     1201 ccgccggggc tgcggccagc acctgcaggc tgccggcatc gcccacgtcc ggcaggtcgc
     1261 cggccggatc gtcttcggtg tcctcggtgc cgtcgggttc gtcgcggccg gcgtcgtcgc
     1321 cggtggccgg atcgggcagc gccgccggca cggcctcgct gccgtcgtca tcgacgtgcg
     1381 ggttcggcag ctccgggggc tgcaccagcg gctggccgag ccggcggccg gcgtcgtcga
     1441 tccaggcggc gatcagtccg gcggccgggg cggcgcgcag cagtgcgatg ccctgaccgg
     1501 cccacagcgg ctgcagctcg ggccggtcct gcgcggccgc ggcgtggcgc agcgccgtcg
     1561 tcagctgctg ctgcaccggc cagaccggca ggttgccggc ctcgctggcc agttcgcgct
     1621 gcaggcggtc atcgagctgg cgcgcgcagc gcccgagcca cagccggccg ttgatgctgt
     1681 cgagttcggt ggccgcgagc aggcgctccc ggcaggccgg actcaggccg gtttccggac
     1741 agccgaggaa ggccgtgccg agcatgacgc cgctggcacc gaggttcagc gccgcggcga
     1801 tgccgcgccc gtccatcagc ccgccggcgg cgaccaccgg cagcgacagg tgatcggcga
     1861 gcagcggcac cagcgcgcag gtgccgagcg cgacgcgctc cggggcgccg gcgaagccgc
     1921 cgcgcaggcc gccggcctcg gcgccctggg cgacgacgat gtgcacgccg gactcttcga
     1981 gcaccagggc ttcgagcaga tgcgtggcgg tgccgatgac ggtgacgccg gcggcgagca
     2041 gcggcgcgag cagctcatcg ggcggcaggc cgccggcgaa gctcgcgacc tcgatgcctt
     2101 catcgagcag cacttcgagt tgcgcctcga acgagggcgg cggcatcggt tcgggcggtt
     2161 cggcgaggcc gagcgcgtcg tggtagggcg caagcagcag gcgcgtgtac tcgatgcggt
     2221 cgccgtcgat cacgtacggc accggcgcgt gcagatggac ggcgaagggc tggtcggtca
     2281 gcgcgcgcac ggcgcagatc gcggcgcgca gcgcctcggg tggcagttcg gcgccgcaca
     2341 ggctgccgag cccgccggca ttggcgaccg ccgcacagag cgccggcgtg ccggccggag
     2401 cggccatcgg ggccagcagc agcggcaggc ggatgcgcag ccgctcacgg agcgcgcggg
     2461 gcgaacaggg catgtcggga ctcctgtcgg gggatcgcgg ccgattatgc ccgcgccgcc
     2521 cgcggatgca tccgtcaggc cgctgccggc agcccgaaca gccggcgggc ggcggcggtg
     2581 ctggcggcgg cgacggctgc gggcgactcg ccgcgcagcg cggcgaccgt ggccagcacc
     2641 tcgggcaggt aggcgggttc gttgcgctcg ccgcggtgct gcacgccggg ctggtccggg
     2701 gcgtcggttt cgagcagcag gccgtcggcc ggcagtgcgc gcaccagccg gtgcaggcgc
     2761 tgcgcgcgct cgtagctcag cggtccgccg aggctgacca ggaagccgtg ctcgcgcagc
     2821 cgctcggcct gctcgatcga gccggcgaag ctgtgcacga cgccgctcag gccctcgcgc
     2881 ccgccgatgc ggcggatgca cttcagcacg gcatcgacgg cgcggcgtgc atgcacgatc
     2941 accggcaggc cggcgtcgcg ggcaatgcgc agctgttcga ggaaatacgt ctcctgcgcg
     3001 ggcaggtccg gttgcggcag gaaggcgtcg agcccgcact cgccgatcgc gaccgggcgg
     3061 cgagcggcga cgcgcgcgcg cagttcggcc agatgcgcgg gccggtgctc ggcgaggtag
     3121 cacgggtgca ggccccaggc cgcgtacagg ccggggtagc gcgcgaccac ggcatcgagc
     3181 gcgtcccagc tgcgcgcgat gacgccgggc acgacctgct gcacgacgcc ggcggcgcgc
     3241 gcgcgggcat aggcggcatc gcggtcggcg tcgaattcgc cgacatcgaa atggctgtgc
     3301 gaatcgacca gttccggctg cggggccgca gcgggcaggg cggatggatt cacgggcatc
     3361 ggatacggtc cttgccggac gcggacgccc ggcgtgctgg ctatgctgcc ggagtcgcag
     3421 ccgcggagaa ctaggggatg accggccggc tgtctgtcac cggccgttcc cgtcgtggag
     3481 gtccatgatg cgcccttcgt tccctgtact gacgatgttc gccgcgctgg ccgctgccgg
     3541 tccggtcgcg gccggcgtgc tcgctgattt cgaaggtctg gcgacccctc cgccggtcga
     3601 tggcgctacc ggcttcgagt tcgccaacgg cggcagcctc gattacgccg gcgtgcgctg
     3661 ggatgaccgt attcaagtgg tcggcgatgc ctaccgcgtc gacaccgcaa cgccggggcc
     3721 gctgttcggg ctgccgcatt cgggcagctt cttcatcaac aaccacaacg gcgccgacgg
     3781 catcctgctc gaaaccacgc aggtgctgac cggcgcctgg ttcgcgcgca acgagtatta
     3841 cggcttcggc ggcggtgccg atcaggtgtc gatcaccgcg ctcggcgcgg gcggcgacct
     3901 gctgacgctg agcctcgatc tgccggagct gctggcgggc ctgcccgagc agccgggctt
     3961 cttcgacacc tcggccttcc tcgcgctgtc cggcatcacc ggctatcgca tcgaccggcg
     4021 cgagaccggg ccgttctcgg ccgactgggt cgccgatgac ttcacgttcg aggcggccgt
     4081 gaccgcgatt ccggcgccat cggtgctggc gctgttcggc ctcggcggcc tcgggctctg
     4141 ccggcgccgg cgctgaagcc gcggaagggt cgggcgggtc gcggtgtagc aggggctgcg
     4201 gacagcgtcg tccgtgccat ccgaaccttc cggagcctgc gccatgaagc cgcacccgct
     4261 gaccttcgtc ctgcccgccc tgctgctcgc cgcctgcgcc gccagtgatc ccggcgcggc
     4321 tccggcccgc gtcgccgatg gcgtctgggt cggtggcaac ggcatgacgc tgtacgtgtt
     4381 cgacaaggat gtcgccggtt ccggcaggag tgcctgcaac ggcgcctgcg ccgcgaactg
     4441 gccgccgctc gcggccgccg ccgatgcccc ggccggcggc gactggacga cgatccggcg
     4501 cgacgacggc agccgccagt gggcctggcg gggccggccg ctgtacttct ggagcaagga
     4561 cacgcagccg ggccagcgca gcggcgacgg cttcaaccag gtctggcacg tcgcgcgcgc
     4621 cggctgaggc cgctcaggcg ctgtgcgcgg cgtcggcgtc ggcccaggcc tggcgcttgc
     4681 ggtagatggt cgaggcgctg atctccagca gcgccgcggc cttcgggatg ttgccgtcgc
     4741 aggcggcgat ggcatcctcg atcagctcgc gctcgacctg ctccagcggc cggatgcgca
     4801 gcgtcggatc gccgggcggc agctccgcgg cgagcgccgc cgccggcggg acttcgcggg
     4861 attcgctgcg cgcggtgacc gctgcctcgc cggcggcgac cacccgcgac agcggcgccg
     4921 gcagcatctg cggcgtgacc tcgccggcgt cgttgagcac gacgatgttg cgcacgacgt
     4981 tctgcagctc gcgcacgttg ccgggccagc cgtattcgag cagcacggcc tcggtgtcgg
     5041 cgcggaagcc ctggaagcgc cggccttcct cggccgcgta ctcgacgagg aagtgccggg
     5101 cgatcgcgag cacgtcgtcc tcgcgctcgc gcagcggcgg caggtgcagc gggatcacgt
     5161 gcaggcggta gtacagatcc tcgcggaagc gcccttcgtc gacttcctgg cgcggatcgc
     5221 ggttggtcgc gcagatgaag cgcacgtcga ccttctcgat gcgtccggaa ccgaccggct
     5281 ggaagctgcc ggtctgcacg aagcgcagca gcttggtctg cagctcggcc ggcatctcgc
     5341 agagctcgtc gaggaacagc gtgccgccgt cggcgagcgc ggccgcgccc ttgcgctcgc
     5401 tggtggcgcc ggtgaaggcg cccttcacgt ggccgaagat ctcgctctcc atcaggtcgc
     5461 gcgggatcgc cgcgcagttg agcgcgatga acgggccgtc gcggcgtgga ctctggcggt
     5521 ggatggcctc ggcgcagatt tccttgccgg tgccgctctc gccggtgatg aacacggtcg
     5581 cgcggctcgg ggccgcggcc tcgatgatcc ggtacaccgc ctgcatcggc agcgaactgc
     5641 cgatgaagcc gcagtagccg tcgcgggcac cgtcgcgcag cgtcgcgacc agctggtcga
     5701 gccgctggcg ctccagggcg ttgcgcacgg tcaggcgcag gcggtcggcg tcgaactgcg
     5761 tcttgaccag ataatcccag gcgccgtcgc gcatcgcctg cacggcgagc agcaccgaat
     5821 cgttggcggt gatgacgacg acggcgaccg gcagcccgcg cgcgaccacg gcgcgcagga
     5881 tctccatgcc gttcatgtcc ggcaggttca ggtcgagcag cagcgcgtgc ggcggttcgc
     5941 gttcgatgac ggcgagtgcc tcggcaccgg tctcgacgtg gacgagccgc caggcttcgc
     6001 gcttcagata ccccatgtac acctcggcca tcgcgcggct gtcctcgacg atcagcacgc
     6061 ggggccggaa gtgaccgggc agattggcag aaccgctcat catggactcc ctgcgcagcg
     6121 gcgcgcgccg gcgggaacgg cgccggcgga cactgtagtg cgcgccggca gccgcgggaa
     6181 ccgcaggctt gcaagcaggt gtcacgattg cggatcgggg ggcgggaggt ctgcggcccg
     6241 ctgcgctcat cggcccggtc cccgcttcag ctgcggcccg ccggcgcgag cggttcgagc
     6301 agcagcagca ggccgggatc ggcgccgagc aggtcgagca ggcgctcggc ggccatcggc
     6361 agcggctggc gcgcggtgat ctgcgcgagg aagcccggca cgctgccgcc gccggcgagc
     6421 ggcgccggcg cgcggctcat cagcagcagg cgttcgccgg gcgcgagccg gcgttcgagc
     6481 ggtccggcga gttcgagact ctgcgcgaaa ccgccgctgg cgagcaccca ggcgccgacc
     6541 cggccgctgc cggcgagcga cacctgggcg gacgggccga tgtgcacgac cagcgcctcg
     6601 gcgcgcactc cggcgagcac cggatcgacg ctggtgcgcg cgagcagcgt cgccagcgtc
     6661 gccgccggcg tctggccgtg cggcagatgg cgcagcaggc cggcgagata gctcgcgcgc
     6721 agctgcgcgg ccgaaccgta ttcgtcgtcg tcgccggcga gccgcaccag gatcacctgg
     6781 ctcacgcctt cggccggatc gtggccacgg cccgcgtaca ggccgtcgag cagggcgctg
     6841 ccgcgctggc cgctgaccac ccggcagccg ccgaggcgct cgggcagctg cgggcgcagc
     6901 gtccgttcga gctgttcgcg cgcggcatcg atccagcgca ggcgccgggc gcgaccgcgc
     6961 gcgagcacgc ggcgcagcgt gccgagcagt tcgcgcttgc gcagcggctt cagcaggtag
     7021 tcgtcgatgc cgagctggtc ggcatcgcgc ttgaggcgtt catcggcggt gccggtgatg
     7081 aagatgaacg gcagcaggtc ggtgtcgttg cgctcggcga gccgcgcgcg gaaaccgagg
     7141 ccgtcgaggc cgggcaggtg gatgtcggag accacgaggt cgtagcgctg ctgcgcgagg
     7201 gcggcgagcg cggcgtcggc ggcggcgtag ccgatcacgt catagtcgtc ctgcaggtac
     7261 agccggatca gctcgcgctg cgcggcatcg tcatcgagca cgagcacgcg cggccggggc
     7321 gcgggcgcgt cggacgggcc ggcgctgcag tctgcaaacg cccgggccgc gccgggggac
     7381 aggtcgtgca ttttgcttcc ggaatcggca agaggatcgg gggcgggggc cgggacgctg
     7441 cccggtgccg cgatgccctg cctgcacgca ggagccgtgc cagtgccggg tgatccggga
     7501 gccgatgccg ggggcaggcg tgcgctaccg gcgcgggaac agccggtcga gttcgatcag
     7561 cgccggcacc gcgaccgcgc ggatggacag cgcgagttcg cgcgcccgtt cgagctggcc
     7621 ggcgcgacag aagtcgttga gctggcgggc gtgttcctgc agctggcggg cgccgaaggt
     7681 gccggcgctg gatttcagcg tgtgcgcctc gcgctgcagc gtcttccagt catcgcgggt
     7741 cagtgcggcg tcgatcgaat cgaggcgcac gcgcgattcc tcgacgaaca gctgcacgac
     7801 gcgcccgagc acgtcgtgat cggtgtcggc ttcgagctga tcgagcacgg cctgatcgac
     7861 gaccggcacg gccggtgcca cgggcgggga cggaagcggg gacggcggcg gcggcgcggc
     7921 tgccgcggct gcgctcacca ccggctgcgg gccggtgctg ttgccggcgt cattggcggc
     7981 ctcggcggcg cggcgggcgg cgtcggcctc gggttgcgcc agccagcgct ccagcgtcgc
     8041 cagcagctgg gcacggtcga tcggcttggt caggtagtcg tccatgccgg cggcgaggca
     8101 gcgctcgcga tccccgggca gggcgttggc cgtcatcgcg atgatcggca ggtcgcgctc
     8161 ggggccgggc atgtggcgga tctcggcggt ggcctcgaag ccgtccatct ccggcatggc
     8221 cagatccatc agcaccaggt cgtagggcag cgtgcgcacc gcctgcacgg cttcgaggcc
     8281 gttgccgacc gcatcgacct gatagccggc cttcttcagc atcgcgatcg cgacggtctg
     8341 gttgacctga ctgtcctcga ccagcagcac gcggccgaac ttgcgcggcg gcggcgcggc
     8401 cgggaccggc tcgggcgcgg tcaggcggac ttcgctttcc tcgcgccggc gcgcctcctc
     8461 ggcatcgttg aggccgatcg ccaccgccag ccagcggtgc aggctctgct ggcgcaccgg
     8521 cttggtcagc gaggcgtgga agcccatgcg ccgcacgcgc tgcgcgatcg acggcgtgcc
     8581 catggtcgcc atcaggatca gcgccgtgct ctcgagctgc gggtcgcgca ggatgccctg
     8641 gccgagttcc tcgccgcgca tgtcggcgag ccactggtcg atcagcgcca cgtcgaaggg
     8701 ccggccgccg ctgcgcgcgt ccttgagcgt cgcgagcgcc gccgtgccgt tggcgaccgc
     8761 ttcgaccgcc atgccccagc tcgcgagttc gcgctcgcgc agccggcgcg tgaccgggtt
     8821 gtcctcgacg atcagcacgc gcacgccgcc gagcgtggcg atcacgcccg gcgtggtcag
     8881 gcgatcgccc gacgggcagc cgacatcgat cgtgaaccag aaggtcgagc cgaggccggg
     8941 ggcgctggtg aagccgatct cgccgcccat cagctcgacc agccggcgcg agatcgccag
     9001 gccgaggccg gtgccgccgt acttgcgcga gtgcgagggg tcggactgcg agaacttctc
     9061 gaacagccgg gcctgcgcct cgcgcgggat gccgatgccg gtgtcctgaa tttcgaagcg
     9121 cagccggatg cgcccggccg ggcgcgcgac cggttcgcgc gcgacgtgca gcgccacgcc
     9181 gcccttgcgc gtgaacttga cggcgttgcc ggcgaggttc agcaggatct gccgcagccg
     9241 cccggcgtcg gcggtcacgc gcgccggcag gtcgacgccg atccaggtgg cgatctcgat
     9301 gtgcttgccg tgcgcgcgcg gcgccagcag ctcggcgacg ccctcgatca gctgcacgag
     9361 gtcgaactcg gtgctctcca gatcgagctt gccggcctcg atcttcgaga agtcgaggat
     9421 gtcgttgatg atggtcagca gcgccttgcc ggattcgctg gcggtctcgg cgaagttgcg
     9481 ctgctcctcg gtcagcggcg tgtcgagcag cagcgtcagc gtgccgagca cggcgttcat
     9541 cggcgtgcgg atctcgtgac tcatggtcgc gaggaattcg gacttggcgc ggttggcgga
     9601 ttcggcctgg tccttggcga tgcgcagctc ctcctcggcc tggcgccgtt cggtgatgtc
     9661 gcgcaggaag gccgtgaaca gccgctgcgg gccggcctgg atcggcgtca ccgcaatctc
     9721 ggccgggaac tcgctgccgt cggcgcgcag cgccatcacc tcggtgcgcc ggccgatcat
     9781 ctgcgtggcc tcgccggtgt cgaggtagcg cgccatgccg acccggtgct cgctgcgcag
     9841 ccggcccggg atcagcaggt cggccatcgg ctggccgagc acctcggcgc gcgcgtagcc
     9901 gaagatgcgc tcggcggccg gattgaattc gagcagccgg ccgtcctgat cgatcgtgat
     9961 caccgcatcg agtgcggctt cgagcatcgc cgccttcagg ccctcgctgg cgcgcagctg
    10021 cgattcggcg cgcttgcgcc gcgagatgtc gcgcacgatc gccgcgaagt aggcccgccc
    10081 ctcggccgag aaggcgctga cgctcagctc gaccgaggcc agcgagccgt ccttgcggcg
    10141 cacgccgagt tcgctggtgg cgccgggcag gccgcgggtc agcgtgcgca gcggatcatc
    10201 gcccagggtt tcgagcaccg ccgccggcag cagcacgcgg aagttgcggc cgatcacctc
    10261 ggcggcggca tagccggaga gtttttcggc cgccgggttg aagcgcacga tcgtgccgcg
    10321 ctcatcgagc gtcaggatcg cctcggcggc cgcggcgatg accgcggcat cgatcgtcgc
    10381 cggcatcggc tcgccggccc cggaagcggg ttcctcggcc cggaggaagc cgaacaaccg
    10441 gttcgacagg ctgcgctgcg ggggctggtc tttcatgtcg cgacagggat cggtgcggac
    10501 cggcggcgcg cggccatggc ggcccggacg tcggagcgag gcgtccggtt tcctcgggcg
    10561 tccggcgagg ccggggcgcg agcggcagcg tagcgtgcgc cgcgcgcggc tgcgcggatt
    10621 gtcgcggtac gccggggtac tgcccgttca ggccggcgca tacagcgccg cgagccgggc
    10681 cgcggcggcg cgcacggcat cggtgccggc gtggccgaac acggcgccga tcacggccag
    10741 cgcatcggcg ccggccctga ccaggccggc gccgttgccg gcgtcgatgc
//
LOCUS       DAOQIE010000112        10766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3064_length_10766_cov_6.537578, whole genome shotgun sequence.
ACCESSION   DAOQIE010000112 DAOQIE010000000
VERSION     DAOQIE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_10766_cov_6.537578"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1118)
                     /locus_tag="PK144_08630"
     CDS             complement(300..1118)
                     /locus_tag="PK144_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF65831.1"
                     /translation="MMKKQIAVALGAALALGGTSLAHAGYYQYANDFRAHYAFSDFSN
                     ADNVTTLTFSNISGNLEFNIPPAGLWSVSRKGEFDVDYNGTGVPSPFQAASVCNSVGK
                     AGWDFCSNVSSFIPLASVNGSYTDIAGTHFIYNWDTDTFTSDGNAYTVGDFTGSATGI
                     TLLLGAFLGPVGGFIASAIGDGSVTVHQVFDGAANTWVLTLTETSGVGIESILNQLDT
                     GTSGIPGLPAPLQNAMIDGEVFANGTIHIPEPASVALLGLGLAGLATARRRKAA"
     gene            1448..2839
                     /gene="sthA"
                     /locus_tag="PK144_08635"
     CDS             1448..2839
                     /gene="sthA"
                     /locus_tag="PK144_08635"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNF65832.1"
                     /translation="MSEQKYDVIVIGTGPGGEGAAMKATKSGKLVAVVEKYNAVGGGC
                     THWGTIPSKALRHAINRMMEFNRSALYRSLGRAVRFSYPDLLKTAESVIAQQATVRRD
                     FYDRNGIPIYRGTARFVDAHTIELRGAQPVRLRADHFVLATGSRPYRPSDVDFNHPRV
                     FDSDTILSMAYTPESISIYGAGVIGCEYASMFRNMGCKVNLINTRDQLLSFLDDEITH
                     ALNYHLRDQGVVIRHNETYERVEAQSDGVVMHMKSGKRIKSDIVLWANGRTGNAQHMG
                     LEELGIRPNGRGQIDVNAHYQTALDHIYAVGDVIGYPSLASASYDQGRFAATHMADGV
                     CDSRLVEYVPTGIYTSPEISSVGRTERELTEACIPYEVGYAWFKSLARAQITGKTVGM
                     LKLLFHRDTLELLGIHCFGDQAAEIVHIGQAIMAQRGEANSLLYFVNTTFNYPTMAEA
                     YRVAALNGLNRAR"
     gene            2939..4069
                     /locus_tag="PK144_08640"
     CDS             2939..4069
                     /locus_tag="PK144_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNF65833.1"
                     /translation="MDVTPYLKLMVDREASDLFFYVGAPVNIKIEGTVMPLGNVPLKP
                     GAVREIAYSLMTDSQIREFERDLESNFALSVSEIGRFRANVFRQRGEVSMVIRYIKQR
                     IPDFVELSLPQILGNLVMEKRGLVLMVGSTGSGKSTSLAAMIDHRNSNAAGHILTIED
                     PIEFVHHHKKSVIGQREIGIDTHSYGDALKNALREAPDVILIGEVRDAETMKYSISYA
                     ETGHLCLSTLHANHANGALERIINFFPEIARPQLLMDLSQNLRAIVSQRLIIGVDGRR
                     VPAVEIMLNTPYISELILKGKFESIKEAMAQGTEIGMKTFDQALYDLYKGGRISKDEA
                     VRNADSRNNVSLQIRLSEGMGQGKAAPDPLGASLKVTPKDVR"
     gene            4175..5341
                     /locus_tag="PK144_08645"
     CDS             4175..5341
                     /locus_tag="PK144_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF65834.1"
                     /translation="MTRPRIAARMAGIAPFHVMELLARAQALEAAGRDIVHMEVGEPD
                     FVTAAPVVEAGRRALAEGRTAYTAAKGLTALRTAISDDYARRYGVGVDPDCILVTPGA
                     SGALLLAAGVLLDPGERCLLADPSYPCNRHFVRFCGAEALGIPVGADTRFQLTPELVA
                     AHWDARTAAVMLASPSNPTGTCVPPAALAGIHAEVRARGGALIVDEIYQGLVYDGEPA
                     TALAGGEDVFVINSFSKYFGMTGWRLGWLVAPASHVREAEKLAQNLFLAASTPAQHAA
                     LAAFTPEAEAIFVERREAFRRRRDRLVPALRELGFGVPVQPDGAFYVYADAGRHTDDA
                     YAFCFELLEAAGVAITPGIDFGAHEAATHVRFAYTTGLDRIELALERLRRFLGR"
     gene            complement(5413..7110)
                     /locus_tag="PK144_08650"
     CDS             complement(5413..7110)
                     /locus_tag="PK144_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF65835.1"
                     /translation="MNDPLSQTTEFLRGIEAALDPFGIGRACRETVTAWLQQPQQLAE
                     SMARLSADVLRVQAHAWKKALGVESDDVEPVSGFDERFQDPAWTAHPGFDALKEFYLL
                     QTHWLQDAIFATPGLDPASVQRAAFWVRQVLDAIAPTNFFWTNPLAIAKAIESGGTTL
                     RQGLTHFARDLAAGDVLMVEPDAFELGRELAATEGVVVFRNELLEVIQYTAQTPQVHA
                     MPVVLVAPWINKFYILDLKPRTSLVRHLVSQGFTVFVTSWRNAGAEARDTGFEDYLMH
                     GVRAAIDTARAITGSPEVHAVGYCLGGTALSTLMAWYAAGDEPCPVAHWTLFTTLTDF
                     SDPGEIGVFLTPETVDFIERNMQRKGYLDGAEMARSFRLLRPNSLIWHYVVHSYLYGA
                     ELPPFDVLFWNMDTTRMPATMHAWYLRELYLGNRLAKGEITLGGRRLQLGRVQQPLYA
                     VGCEQDHIAPWKQTFLTCGLVGGPVRYVLATSGHILGIINPPVDPPKRRYWVGDASGC
                     SDGPAWLNGVAKVPGSWWEDWVPWLAARCGELRELPPLGTAEYPVLAAAPGTYVLQK"
     gene            complement(7304..7894)
                     /locus_tag="PK144_08655"
     CDS             complement(7304..7894)
                     /locus_tag="PK144_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF65836.1"
                     /translation="MNSLPEQLVDGTAGDSGFDVGARLRAVREANGLSQRELARRAGV
                     TNGTISLIEQNKNSPSVSSLKKVLDGIPMTLAEFFAQDEPPQDQVFFRAAELVTLTRG
                     AVSFRQVGARAGHALQILHEHYAPGADTGRAMLRHDGEEGGIVLRGRIELTVGDRREI
                     LGPGDAYHFSSRIPHRFRNPGDEECELISACTPPYL"
     gene            8264..9370
                     /locus_tag="PK144_08660"
     CDS             8264..9370
                     /locus_tag="PK144_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF65837.1"
                     /translation="MSTQSGGDAFVRFREVRKTYDGEHLVVKNLNLDIGRGEFLTLLG
                     PSGSGKTTALMMLAGFETPTHGEILLDGKPLHNLPPYRRDIGMVFQNYALFPHMTVAE
                     NLAFPLSVRKLPKADIAQKVARALDMVRLGALAGRYPAQMSGGQQQRVALARALVFEP
                     KLVLMDEPLGALDKQLREHMQLEIKQLHAQLGLTVVYVTHDQSEALTMSDRVAVFNDG
                     VIQQIDAPETLYEHPQNSFVAHFIGENNRLAGEVQTIDDRSCTVRLDDGPLVQARAVN
                     VGGVGSRTSASIRPERLRLAGAAQGADNVFEARIRDFIYLGDHLRVRLEFAGSREFTV
                     KVPVSQFDPRWRAGDTLAVGWSADDLRALDPVAA"
     gene            9477..10535
                     /locus_tag="PK144_08665"
     CDS             9477..10535
                     /locus_tag="PK144_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF65838.1"
                     /translation="MQSTKFQSKRILSALLAAGLCSAATLAGAADLTVISFGGSNQKA
                     QEVAFYKPFQASGAGTVVAGEYNGEIAKIKAMVETGNVSWDIVEVEGPELLRGCSEGL
                     YEKLDWAKLGDKSKFVPAAVSECGAGIFVWSTALAYNADKLAKAPASWADFWDVKTFP
                     GKRGLRKGAKYTLEFALLADGVPEADIYKLLATKEGVARAFRKLDELKPHIQWWEAGA
                     QPPQWLAAGDVVMSSAYNGRIAAAQKEGKNLKIVWNGSIYDIDHWTIVKGSKNKDMAM
                     KFIAFANQADRQAVYSQNIPYGPTNNDAIAQIDPKLAADLPTAPANLKGAFATSTEFW
                     VEYGEDLEERFNAWAAGK"
BASE COUNT         1843 a         3537 c         3651 g         1735 t
ORIGIN      
        1 gatggatcgg gccattggcg taaggcagag cgctggtgac gaggatggtg cgcgcagggg
       61 tcgtcatggc gggtgatcgg cggcgggagg caagggcggg aacctagcaa catcgattgc
      121 ccgtggctac cttcttacag cattatttaa tcggataaac cgctcgcaca tgaataagtc
      181 attgatttat ctgattattt agtttttaat gaatgcacaa ttgtgtcttt atggttagac
      241 aattgttgca taaaagaaag cggcggcttg cgccgccgct gtataatgct tctaggtgct
      301 tatgcggcct tgcggcgacg agcggttgcg aggccggcga ggccgaggcc gagtagggcg
      361 accgatgctg gttccggaat atgaattgta ccgttggcga acacttcgcc atcgatcatg
      421 gcgttctgca agggcgccgg taagccgggg atgccagacg ttccggtgtc aagttggttg
      481 aggatgcttt caatgcctac gccggatgtt tcggtcagag ttaggaccca ggtatttgca
      541 gcaccgtcaa atacttgatg tacggtcaca gaaccgtcgc cgatggcgct ggcgatgaat
      601 ccgccgacgg gcccgagaaa cgctcccaga agcagcgtga taccagtggc cgagccagtg
      661 aaatcaccga cggtatacgc gtttccgtca gacgtgaagg tgtcggtatc ccagttgtag
      721 atgaaatggg tgccggcgat gtctgtataa cttccgttta cagatgcaag tggaatgaac
      781 gacgacacat tgctgcaaaa gtcccatccg gccttgccga cgctgttgca cacgctggca
      841 gcctggaacg gcgaggggac accggttcca ttgtagtcga cgtcgaattc acccttgcga
      901 ctcacgctcc acaggccggc tggcggaatg ttgaattcca agttgccgct gatgttgctg
      961 aatgtcagtg tggtgacatt atcggcgttc gaaaaatccg aaaaggcata gtgggcacga
     1021 aagtcattcg catactggta gtagcctgca tgcgcgagac tcgtgcctcc gagcgccaga
     1081 gcggcgccga gcgcgaccgc aatctgcttc ttcatcatgt gtttagccct ttggttcaga
     1141 tcgtaaggcc ggaagccagg agcgcggcgc cagtccgcgg gaattagcct cagtcctgag
     1201 agcagcgcgc tgtctcctgt caggcaagca atgggccatg attgataaca tgatgaatat
     1261 aaagggatgt agctagggtg atatgggaga gggtgaaccc acatgtaaaa taatctgact
     1321 gcagtggagg ccgccggccg ggggggcggg cggcttgtcg gcgtgttgcc ggtatactgc
     1381 ggcgcaccgg ccggtcatgg cgtgcatccg gcggcatgcc gcaggtcaac atcagcaagg
     1441 ggcagagatg agcgagcaga agtacgacgt gatcgtgatc ggcacgggac cgggcggcga
     1501 aggggcggcg atgaaggcca ccaagtcggg caagctggtt gcagtggtcg agaagtacaa
     1561 cgccgtcggt ggcggctgca cgcactgggg cacgatcccg tccaaggccc tgcgccatgc
     1621 gatcaaccgg atgatggagt tcaaccgcag cgcgctgtac cgcagcctcg gccgggcggt
     1681 gcggttttcg taccccgacc tgctgaagac ggccgagtcg gtgatcgcgc agcaggcgac
     1741 cgtgcgccgc gacttctacg accgcaacgg catcccgatc tatcgcggca ccgcccgctt
     1801 cgtcgatgcg cacacgatcg agctgcgggg cgcccagccg gtgcggctgc gcgcggatca
     1861 cttcgtcctc gccaccggtt cacgtccgta ccggccgtcc gacgtcgact tcaaccatcc
     1921 gcgcgtgttc gacagcgaca cgatcctgag catggcctac acgccggagt cgatctcgat
     1981 ctacggcgcg ggcgtgatcg gctgcgaata cgcgagcatg ttccgcaaca tgggctgcaa
     2041 ggtgaacctg atcaacaccc gcgaccagtt gctgtccttc ctcgatgacg agatcacgca
     2101 cgcgctgaac tatcacctgc gcgaccaggg ggtcgtgatc cgccacaacg agacctacga
     2161 gcgcgtcgag gcgcagtccg acggcgtggt catgcacatg aagtccggca agcgcatcaa
     2221 gagcgacatc gtgctgtggg ccaacggccg caccggcaac gcccagcaca tggggctcga
     2281 agaactcggc atccgcccga acggccgcgg ccagatcgat gtcaacgcgc actaccagac
     2341 ggcgctcgat cacatctacg cggtcgggga tgtcatcggc tatccgagtc tcgccagcgc
     2401 ctcctacgat caggggcgct tcgccgcgac gcacatggcc gacggcgtct gcgacagccg
     2461 gctcgtcgag tacgtgccga ccggcatcta cacctcgccg gagatcagct cggtcgggcg
     2521 caccgagcgg gagctgaccg aggcctgcat cccgtacgag gtcggctatg cgtggttcaa
     2581 gagcctcgcg cgtgcgcaga tcaccggcaa gaccgtcggc atgctgaagc tgctgttcca
     2641 ccgcgatacg ctggaactgc tcggcatcca ctgcttcggc gatcaggccg ccgagatcgt
     2701 gcacatcggc caggcgatca tggcgcagcg cggcgaggcc aattcgctgc tgtacttcgt
     2761 caacacgacc ttcaattatc cgaccatggc cgaggcgtac cgtgtcgcgg ccctgaacgg
     2821 actgaaccgc gcccgctgat ccgcagcggg cgaggcaggt cgtggcggca ggcagcggct
     2881 tcgcgcaggc gcctggtgcg ccgcggcggc ggcagtgatc gatgggggga ggcagtccat
     2941 ggatgtcaca ccgtatctga agctgatggt ggaccgcgag gcctcggatc tcttcttcta
     3001 cgtgggggcg ccggtcaaca tcaagatcga aggcacggtc atgccgctcg gcaacgtgcc
     3061 gctgaagccc ggtgcggtgc gcgagattgc ctactcgctg atgaccgaca gtcagatccg
     3121 cgaattcgaa cgcgacctcg aatcgaactt cgcgctgtcg gtcagcgaga tcggccgttt
     3181 ccgcgccaac gtcttccgcc agcgcggcga ggtgtcgatg gtcatccgct acatcaagca
     3241 gcggattccg gatttcgtcg agctgagcct gccgcagatc ctcggcaatc tggtcatgga
     3301 gaagcgcggg ctggtgctga tggtcggttc gaccggctcg ggcaagtcaa cctcgctcgc
     3361 ggcgatgatc gatcaccgca acagcaatgc cgccggccac atcctgacca ttgaagaccc
     3421 gatcgaattc gtgcatcacc acaagaagtc ggtgatcggc cagcgcgaga tcggcatcga
     3481 cacgcacagc tacggcgatg cgctgaagaa cgcgctgcgc gaggcgccgg acgtgatcct
     3541 gatcggcgag gtgcgcgacg ccgagacgat gaagtactcg atcagctacg ccgagaccgg
     3601 ccacctgtgc ctgtcgacgc tgcacgccaa ccatgccaac ggcgcgctgg agcgcatcat
     3661 caacttcttc ccggaaatcg cgcggccgca gctgctgatg gacctgtcgc agaacctgcg
     3721 cgcgatcgtg tcgcagcgcc tgatcatcgg ggtcgacggc cggcgcgtgc cggcggtcga
     3781 gatcatgctg aacacgccgt acatctccga gctgatcctc aagggcaagt tcgagagcat
     3841 caaggaggcg atggcgcagg gcaccgagat cggcatgaag accttcgacc aggcgctgta
     3901 cgacctgtac aagggcgggc gcatcagcaa ggacgaggcc gtgcgcaacg ccgattcgcg
     3961 caacaacgtc agcctgcaga tccgcctgtc cgaaggcatg ggtcagggca aggcggcgcc
     4021 ggacccgctc ggtgcctcgc tgaaggtcac gccgaaggac gtgcgctgag cgcgcacccg
     4081 ccgttttccc gcccgcgcga ccgcagcccg ctgcggtcgc gtcgttttgg cggacctgaa
     4141 ggttcatgcc gatgactgtc gaggaagcct cccgatgacc cgtccccgca tcgccgcacg
     4201 gatggccggc atcgccccgt ttcacgtcat ggaactgctc gcccgcgccc aggcgctcga
     4261 agccgccggc cgcgacatcg tgcacatgga ggttggtgag ccggatttcg tcacggccgc
     4321 gccggtcgtc gaggccggcc ggcgtgcgct cgccgagggg cgcaccgcgt acacggccgc
     4381 caaggggctc acggcgctgc gcacggcgat cagcgatgac tacgcacgcc gctacggcgt
     4441 cggcgtcgat ccggactgca tcctcgtcac gcccggtgcc agcggtgcgc tgctgctcgc
     4501 cgccggcgtg ctgctcgatc cgggcgagcg ctgcctgctc gccgatccga gctatccgtg
     4561 caaccggcat ttcgtgcgct tctgcggcgc cgaggcgctc ggcatcccgg tcggggccga
     4621 cacccgcttc cagctgacgc cggaactcgt cgccgcgcac tgggatgcgc gcaccgcggc
     4681 ggtgatgctc gcctcgccgt cgaatccgac cggcacctgc gtgccgccgg cggccctggc
     4741 cggcatccac gccgaggtgc gcgcgcgcgg cggggcgctg atcgtcgacg agatctatca
     4801 gggtctggtc tacgacgggg agccggcgac ggcgctcgcc gggggcgagg acgtgttcgt
     4861 gatcaacagc ttctcgaagt acttcggcat gaccggctgg cggctcggct ggctggtggc
     4921 gccggcctcg cacgtgcgcg aggccgagaa gctcgcgcag aacctgttcc ttgccgcctc
     4981 gacgccggcc cagcacgccg cactcgcggc cttcacgccc gaggccgagg cgatcttcgt
     5041 cgagcggcgc gaggccttcc gccgccggcg cgaccggctg gtgccggcgc tgcgcgaact
     5101 cggcttcggc gtgccggtgc agccggacgg cgcgttctac gtctacgccg acgccggccg
     5161 gcacacggac gatgcctacg cgttctgctt cgaactgctg gaggccgccg gggtggcgat
     5221 cacgccgggc atcgacttcg gtgcgcacga ggccgcgacg catgtgcgct tcgcgtacac
     5281 gaccgggctg gatcgcatcg aactcgcgct ggagcgtctg cgccgttttc tcgggcgctg
     5341 aggcggatca gtcgcgggcg tgcaggggct gcgaaatccc ctgccgccgg tgagccgtga
     5401 gtgcgtgcgc ggctacttct gcagcacgta ggtgccgggc gctgcagcga gcaccggata
     5461 ttcggcggtg ccgagcggcg gcagttcgcg cagttcgccg cagcgtgcgg cgagccacgg
     5521 cacccagtcc tcccaccacg agcccggcac cttggcgacg ccgttgagcc aggccgggcc
     5581 gtcgctgcag ccgctggcgt cgccgaccca gtagcggcgc ttcggcggat cgaccggcgg
     5641 attgatgatg ccgaggatgt ggccggaggt cgcgagcacg tagcgcaccg gtccgccgac
     5701 caggccgcag gtgaggaagg tctgcttcca cggcgcgatg tggtcctgct cgcagccgac
     5761 cgcgtacagc ggctgctgca cgcgcccgag ctgcagccgc cggccgccga gcgtgatctc
     5821 gcccttcgcg agccggttgc cgaggtacag ctcgcgcagg taccaggcgt gcatcgtcgc
     5881 cggcatgcgc gtcgtgtcca tgttccagaa cagcacgtcg aacggtggca gctcggcgcc
     5941 gtacaggtag ctgtgcacga cgtagtgcca gatcaggctg ttcgggcgca gcagccggaa
     6001 gctgcgcgcc atctcggcgc cgtcgagata gcccttgcgc tgcatgttgc gttcgatgaa
     6061 gtccacggtt tccggcgtca ggaagacgcc gatctcgccg gggtcggaga agtcggtcag
     6121 cgtcgtgaac agcgtccagt gcgcgaccgg gcagggctcg tcgccggcgg cgtaccaggc
     6181 catcagcgtc gacagcgccg tgccgccgag gcagtagccg accgcgtgca cttcgggcga
     6241 gccggtgatc gcgcgcgcgg tgtcgatcgc cgcgcgcacg ccgtgcatca ggtagtcctc
     6301 gaagccggtg tcgcgtgcct cggcgccggc gttgcgccag ctggtcacga acaccgtgaa
     6361 gccctgggat accagatggc gcacgaggct ggtgcgcggc ttcaggtcga ggatgtagaa
     6421 cttgttgatc cacggtgcga ccagcacgac cggcatcgcg tggacctgcg gcgtctgcgc
     6481 cgtgtactgg atcacttcca gcagctcgtt gcggaacacc acgacgcctt cggtggcggc
     6541 cagctcgcgg ccgagttcga aggcatcggg ctcgaccatc agcacgtcgc cggcggcgag
     6601 gtcgcgggcg aagtgggtca ggccctggcg cagcgtcgtg ccgccgcttt cgatggcctt
     6661 ggcgatcgcg agcggattgg tccagaagaa gttggtcggc gcgatcgcat cgagcacctg
     6721 ccgcacccag aacgccgcgc gctgcaccga ggccggatcg aggccggggg tggcgaagat
     6781 cgcgtcctgc agccagtgcg tctgcagcag gtagaactcc ttcagcgcat cgaagcccgg
     6841 atgtgcggtc caggccgggt cctgaaagcg ctcgtcgaaa cccgagaccg gctcgacatc
     6901 atcggactcg acgccgagcg ccttcttcca ggcgtgcgcc tgcacgcgca gcacgtcggc
     6961 actgaggcgc gccatcgact cggcgagctg ctgcggctgc tgcagccagg ccgtgaccgt
     7021 ctcgcgacag gcacggccga tgccgaacgg atcgagtgcg gcttcgatgc cgcgcaggaa
     7081 ctcggtggtc tgggacaggg gatcgttcat cagcgacggc tccggaaccg ggcggaatca
     7141 acccggtggc acgtgcggga ttgcggggca cagagcgtag aggccgcggt cgtggccggc
     7201 gagccgtcag gccctcattc cggctgtcat cggcggcgcg cgcacgatgt ttacagaaag
     7261 tcgcgcgccc ggctcacgga gacccgcgca gatctcgcag atatcacaga tacggcggcg
     7321 tgcaggccga gatcagctcg cattcctcgt cgccggggtt gcggaagcgg tgcgggatgc
     7381 ggctggagaa gtggtacgca tcgccggggc cgagaatctc gcgccggtcg ccgaccgtca
     7441 gctcgatgcg tccgcgcagc acgatgccgc cttcctcgcc gtcgtggcgc agcatcgcgc
     7501 ggccggtgtc ggccccgggc gcgtagtgct cgtgcaggat ctgcagcgcg tggccggcgc
     7561 gggccccgac ctgacggaag gacacggcac cgcgcgtcag cgtcaccagc tcggcggcgc
     7621 ggaagaacac ctggtcctgc ggcggttcgt cctgcgcgaa gaactcggcg agcgtcatcg
     7681 ggatgccgtc gagcaccttc ttcagcgacg agaccgaggg gctgttcttg ttctgctcga
     7741 tcagcgagat cgtgccgttg gtgacgccgg cgcggcgcgc cagttcgcgc tgcgacaggc
     7801 cgttggcctc gcgcaccgcg cgcagccgcg cgccgacatc gaagccgctg tcgcccgcgg
     7861 tgccgtccac gagctgttcc ggaaggctgt tcatcgtttg tcccgttgcg ccgccacccc
     7921 ggtcggtgcc ggctacgctg tcaacccgga cgccccggag cggggcgaag cgctgcacct
     7981 gtctggtgca gcgccggatg tgccggtcac ggtgcgcaac cgtggacgcg acaggcggcg
     8041 gattttaccg ggttcccgtc cgattggctc gaaataataa acgcttcatt gcggaacaac
     8101 gggcgtatcg cagggttgcc acggtttatg tggctttgcg ttgttgagtt ttttaaacac
     8161 tgttagcctg caccgcacat gataactgcg cttcggccga ggggcaccga ccggtgtcct
     8221 tgacagccgc agccgtgaca gcccccagcc aaagaggcgc cacatgagca cgcagtccgg
     8281 cggcgacgcc ttcgtccggt tccgcgaggt ccgcaagacc tacgacggcg aacacctggt
     8341 cgtgaagaac ctgaacctcg acatcggccg cggcgagttc ctgacgctgc tcgggccctc
     8401 gggttccggc aagaccacgg ccctgatgat gctggccggt ttcgagacgc cgacgcacgg
     8461 cgagatcctg ctcgacggca agcccctgca caacctgccg ccgtaccggc gcgacatcgg
     8521 catggtgttc cagaattacg cgctgttccc gcacatgacg gtggccgaga acctcgcctt
     8581 cccgctgtcg gtgcgcaagc tgccgaaggc cgacatcgcg cagaaggtcg cgcgcgcgct
     8641 cgacatggtc cgcctcggtg cgctggccgg gcgctacccg gcgcagatgt ccggcggcca
     8701 gcagcagcgc gtggcgctcg cccgggcgct ggtgttcgag ccgaagctgg tgctgatgga
     8761 cgagccgctc ggggcgctcg acaagcagct gcgcgagcac atgcagctgg agatcaagca
     8821 gctgcacgcg cagctcggcc tgaccgtcgt ctacgtcacg cacgaccaga gcgaggcgct
     8881 gacgatgtcg gaccgcgtcg cggtgttcaa cgacggcgtg atccagcaga tcgacgcccc
     8941 cgaaaccctg tacgaacacc cgcagaactc cttcgtcgcg cacttcatcg gcgagaacaa
     9001 ccgcctggcc ggcgaggtgc agacgatcga tgaccgcagc tgcaccgtgc gcctcgacga
     9061 cggtccgctg gtgcaggcgc gggccgtcaa cgtcggcggc gtcggcagcc gcacctcggc
     9121 ctcgatccgg cccgagcgcc tgcgtctggc cggcgccgcg cagggcgccg acaacgtctt
     9181 cgaggcgcgc atccgcgact tcatctatct cggcgatcac ctgcgcgtgc gtctcgagtt
     9241 cgccggcagc cgggaattca cggtcaaggt gccggtgtcg cagttcgacc cgcgctggcg
     9301 tgccggcgac accctcgccg tcggctggtc ggccgacgac ctgcgcgcgc tcgatccggt
     9361 cgccgcctga tccaccgctt ccgcaacaac gacggacggc ggccagcccg gcgccgcccg
     9421 cagccgccgg tggcgcagca cgccggtcct tcaaccctca gggggagcag caaatcatgc
     9481 agtccacgaa gttccagtcc aagcgcatcc tgtccgccct gctcgccgcc ggcctgtgct
     9541 cggccgcgac gctggccggg gccgccgacc tgaccgtcat ctccttcggc ggcagcaacc
     9601 agaaggcgca ggaagtggcc ttctacaagc ccttccaggc gagcggcgcc ggcactgtcg
     9661 tcgccggcga gtacaacggc gagatcgcca agatcaaggc gatggtcgag accggcaacg
     9721 tctcgtggga catcgtcgag gtcgaaggcc ccgagctgct gcgcggctgc tccgagggcc
     9781 tgtacgagaa gctcgactgg gccaagctcg gggacaagtc gaagttcgtg ccggcggcgg
     9841 tcagcgagtg cggtgccggc atcttcgtgt ggtcgacggc gctcgcctac aacgccgaca
     9901 agctcgccaa ggccccggcc tcgtgggcgg acttctggga cgtgaagacc ttcccgggca
     9961 agcgcggcct gcgcaagggc gccaagtaca cgctcgaatt cgcgctgctc gccgacggcg
    10021 tgccggaagc cgacatctac aagctgctcg cgaccaagga aggcgttgcc cgcgccttca
    10081 ggaagctcga cgagctgaag ccgcacatcc agtggtggga ggccggtgcg cagccgccgc
    10141 agtggctcgc cgccggcgac gtggtgatgt cctcggccta caacggccgc atcgccgctg
    10201 cgcagaagga aggcaagaac ctcaagatcg tctggaacgg cagcatctac gacatcgacc
    10261 actggacgat cgtcaagggt tcgaagaaca aggacatggc gatgaagttc atcgccttcg
    10321 cgaaccaggc cgaccgtcag gccgtgtact cgcagaacat cccctacggt ccgaccaaca
    10381 acgacgccat cgcgcagatc gatccgaagc tcgccgccga cctgccgacc gcgccggcca
    10441 acctcaaggg cgcgttcgcg accagcaccg agttctgggt cgagtacggc gaggatctcg
    10501 aagagcgttt caacgcctgg gccgccggca agtaagcgcg cctgaagcga gcatcctgcc
    10561 tttcctgagc cgcgcaggcg gcatggggat cgacaccagc gacaggttcc ggacggttcc
    10621 tgcgcaccgg ctccggtcgc accggaccgt cagggtgatg caatggcttc gaggcaagcc
    10681 cctcccccct cgtgggggag gggttgggga gagggggaac gcccgggcgc agtgccagcg
    10741 gcttcccccc cccccccccc cccccc
//
LOCUS       DAOQIE010000113        10754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3069_length_10754_cov_7.647631, whole genome shotgun sequence.
ACCESSION   DAOQIE010000113 DAOQIE010000000
VERSION     DAOQIE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_10754_cov_7.647631"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PK144_08670"
     CDS             complement(<1..1725)
                     /locus_tag="PK144_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HNF65839.1"
                     /translation="MCGICGELRLDGNRPDPDLLQRMTDRLARRGPDSAGHWQDGPLA
                     FGHRRLAIIDLSARSNQPMLDAAAGLAIVFNGTIYNYRELRAELQGRGHTFASSGDTE
                     VILKAYAEWGERCVERLIGMFAFAIRNLRTQELFLARDRLGIKPLYYARTPAAFRFAS
                     NVQALLEAADIDTGVDPVALHFQLTLHAVVPAPRTVLAGVRKLPPATTLTILADGRER
                     QQTYWTLAAARPVMARSDDEWLEAVHERLLTAVRRRMDVADVPVGVLLSGGLDSSLLV
                     ALLAEAGKEKLLTFSVGFEDQPEEKGSEFEYSDQVVARYATDHHKFLIPNGEVLKRLP
                     EAVTCMAEPMVGQDAVAFFLLSEQVSKHVKVVQSGQGADEVFAGYFWYPQMAAAEGED
                     SLARFASRYFDRDHAEFLQAVEPHWHGADHSSEHIRRLLEAPGADTYLDRVLRMDVTT
                     LIVDDPVKRVDNMTMAWGLEARVPFLDHELVELAMQLPPELKIGGDGKYVLKKIARGL
                     LPDAVIDRPKGYFPVPALKFVRGPFLDFMREVLDSRACRERGLLQRPYLDTLLADAPP
                     AAQTGGGNR"
     gene            1976..2557
                     /locus_tag="PK144_08675"
     CDS             1976..2557
                     /locus_tag="PK144_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNF65840.1"
                     /translation="MAHELPALPYAKDALQPHISAETLEYHYGKHHNAYVTNLNNLIK
                     GTDFETLSLEDIVLKSSGGIFNNAAQVWNHTFYWNCLSPNGGGEPAGALADAINAKFG
                     SFAAFKEEFSKCAVGTFGSGWAWLVKNADGSVELMSTSNAGTPMTSGKTALLTCDVWE
                     HAYYIDYRNLRPKYVEAFWNLVNWEFAAAQYGA"
     gene            2916..3653
                     /locus_tag="PK144_08680"
     CDS             2916..3653
                     /locus_tag="PK144_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65841.1"
                     /translation="MRTDHQLLEQLLELHEPWEIRAVRTDIHGRRCEVEVGLAAPRTW
                     FGLVRKPSAPAVERGEWRHLNLAGFRYVVRVVAPASVSLERYEWTGEPEQPFTRAMSA
                     RLTRLLAEGVSLEGICRLFGVDPGEIWRLKRSLETRSVRVSPLDAAGAAPDTGAGPAA
                     GAAPGLPEADDPVWGRLAGGDISIDIGVLSLKLLLTRVRSQYEAMPDDESRRLKAREL
                     HRFFVKNLRMLGAELQQLRASALREEA"
     gene            3656..4675
                     /locus_tag="PK144_08685"
     CDS             3656..4675
                     /locus_tag="PK144_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65842.1"
                     /translation="MNSGTTLPVRAPGADLGDLLDVDAVSALILAGERLLLAGDEALL
                     ARLPRGTWIGGTIPYFVAADGGTSSRERLFCTRLPAEVEVDSIRLYDAASLAHVCADA
                     PAEGFSVMILPAFSEVHSLFARNAPNYEDMYLKPLIGWIAGVHLDDLGRATPKVFDGS
                     RGEGCEERAVVLHARLPEPRYARIEIVNLFRPGSGPGLRFTDKGFSAAKVLVDGIEQD
                     FAAWLRRQGTDTRLPLVADYCGAMVNVSIKSVEAGRVDFYAPVFPDIEYRIAAPIDDY
                     VARFLAALPADVPPVTFSCNCILNYLYSNLEGRHTGPMRGPATFGEIAYQLLNQTLVY
                     LTIEG"
     gene            4777..6099
                     /gene="aroA"
                     /locus_tag="PK144_08690"
     CDS             4777..6099
                     /gene="aroA"
                     /locus_tag="PK144_08690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNF65843.1"
                     /translation="MSETRFVVEPGGRLTGRIRVPGDKSISHRSIMFGSLADGVTEVS
                     GFLEGEDCLATLNAFRAMGVRIDGPAAGRVTIHGVGLHGLKPPAGPLDMGNSGTSMRL
                     MSGILSGQAFDTVLTGDASLSKRPMKRVTAPLAQMGARIETTERGTAPLHIRGASGLH
                     GIDYVLPMASAQVKSCLLLAGLYASGTTRVTEPAPTRDHTERMLEGFGYPLVREGERT
                     FSVGGGHRLGATAIDVPADISSAAFFLVGASIAPGSDLTLEHVGINPTRTGVIDILRL
                     MGADIEVQNPRTVGGEPVADLRVRAAPLRGIRIPEALVPLAIDEFPVLFVAAACAAGE
                     TVLTGAEELRAKESDRIQVMADGLVAMGIDARPTADGIVIQGGAFARGAEIATHHDHR
                     IAMSFTIAGLRGAGTHVIRDAGFVDTSFPGFVELARTAGIDVRAERSA"
     gene            6096..6797
                     /gene="cmk"
                     /locus_tag="PK144_08695"
     CDS             6096..6797
                     /gene="cmk"
                     /locus_tag="PK144_08695"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNF65844.1"
                     /translation="MKETVPVIAVDGPSGVGKGTLCRSLAEALGFHLLDSGSLYRLTA
                     LAAARAGLRDDDAGGLAQVAATLDVRFDSGEDDLVVLLAGEDVTAAIRTESAGMAASK
                     VAIVPEVRAALLQRQRDFRRAPGLVADGRDMGTVVFPDAPLKIFLVASPAERARRRHK
                     QLSEKGVDANLAALETEIAERDARDAGRASAPLKPAPDAVILDTDDLGIDAVRDRALA
                     LVAGRLHVPGQQDRS"
     gene            6950..8620
                     /gene="rpsA"
                     /locus_tag="PK144_08700"
     CDS             6950..8620
                     /gene="rpsA"
                     /locus_tag="PK144_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNF65845.1"
                     /translation="MSESFAELFEQSLSQSQMVPGSIVMGKVVDIRGDSVVVNAGLKS
                     EGLIPLEQFLNDDGVIEVKIGDEVEVALDAIEDGFGETRLSREKAKRARVWSVLEGAF
                     EGDEIVRGTISGKVKGGFTVDIGEIRAFLPGSLVDVRPVRDAAYLEGKELEFKVIKLD
                     RRRNNVVVSRRAVVEQEYSAEREALLESLQEGQIVKGVVKNLTDYGAFLDLGGIDGLL
                     HITDMAWKRVKHPSEVVAVGDEVEVKVLKFDRERNRVSLGLKQLGDDPWVALARRYPV
                     GTRLFGKVTNIADYGCFVEIEEGVEGLVHVSEMDWTNKNVNPGKVVSIGDEVEIMVLD
                     IDEDRRRISLGMKQCQPNPWDEFCATHNKGDRVSGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVPGEEAVRDYKKGQEVEAVVLAVDPERERISLGIKQLEKDPFSNFVADNPK
                     GSIVKGRVASVDARGAVIDLGNGVEGTLRVSELARERIEDARTVLKDGEEIEAKFIGV
                     DRKNRTITLSIKAKEYAEEAEVLQDYSRKSATGATLGDLLKEQMGRED"
     gene            8747..9076
                     /locus_tag="PK144_08705"
     CDS             8747..9076
                     /locus_tag="PK144_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNF65846.1"
                     /translation="MTKSELIELLARKQSHLSYKDVENAAKVMIEQMSQSLSGGDRIE
                     IRGFGSFSLHFRPPRVGRNPKTGDAVTLPGKFVPHFKPGKELRERVNASIENDDPRPT
                     ARIAEPA"
     gene            9200..9487
                     /locus_tag="PK144_08710"
     CDS             9200..9487
                     /locus_tag="PK144_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNF65847.1"
                     /translation="MFRIVGILLFLLVLVVGIEFSAVNSAPVTVNYLLGTVSLPLALV
                     VVAAFTVGILVAVAVGTLAMVPLRLRAASLRREVEARQFEIERLRARQPGT"
     gene            9498..10655
                     /gene="lapB"
                     /locus_tag="PK144_08715"
     CDS             9498..10655
                     /gene="lapB"
                     /locus_tag="PK144_08715"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNF65848.1"
                     /translation="MLELLWLLLPVAAASGWWAAARSLERKETPPNDVFRGLNYLLDE
                     KPDRAIEVFGRMVEADPDAVDIQLTLGSLFRRRGEVDRALHIHSELLRRAQLSAGQRG
                     RARLELGEDYLRAGLFDRAEQLFTELLELPDYRSRALVRLLSIYEQEREWQRALDACD
                     EVERATGTPMRIVAAHYCCELAEEAVRASDRATARSWLDRALVHDERCARAAILRGRL
                     ELSDGNPAGALDAYTRVVTRAPEYLPEVLGPLQRCYAQLHRQADWVTLLRGLVNQYHV
                     GSVTGALAQALREREGPEAALVFLEAEVAQHPSTEGLRTLIDLRLAAEQPARAGDLEL
                     LLRIGRQVTDLGPRYRCDNCGFVGRTLHWSCPGCKRWNTVKPIPDRLRRNP"
BASE COUNT         1712 a         3808 c         3615 g         1619 t
ORIGIN      
        1 gcggtttccc ccgccggttt gagccgcggg cggcgcatcg gccagcagcg tgtccagata
       61 gggacgctgc aacaggccgc gttcccggca ggcgcgcgaa tcgagcacct cgcgcatgaa
      121 gtcgagaaac ggcccgcgca cgaacttcaa ggccggcacc gggaaatagc ctttggggcg
      181 gtcgatgacc gcgtcgggca gcaggccgcg ggcgattttc ttcagcacat acttgccgtc
      241 gccaccgatc ttcaactccg gcggcaattg catggccagt tccaccagtt catggtcgag
      301 gaacggcacg cgcgcttcca ggccccaggc catggtcatg ttgtcgacgc gcttgaccgg
      361 gtcatcgacg atcagcgtcg tcacgtccat gcgcagcacg cgatcgaggt aggtgtcggc
      421 cccgggcgct tcgagcaggc gccggatgtg ctcgctgctg tgatcggccc cgtgccagtg
      481 cggctcgacc gcctgcagga actccgcgtg atcgcggtcg aagtagcgcg acgcgaagcg
      541 cgcgaggctg tcctcgccct cggccgcggc catctgcgga taccagaagt agccggcgaa
      601 gacctcgtcg gccccctgcc cgctctgcac gaccttgacg tgcttcgaga cctgctcgga
      661 cagcaggaag aaggccaccg cgtcctggcc gaccatcggc tcggccatgc aggtgacggc
      721 ctcgggcagg cgcttgagca cctcgccgtt cgggatcagg aacttgtggt gatcggtcgc
      781 gtagcgcgcg accacctgat cggaatactc gaactccgag cccttctcct cgggctggtc
      841 ctcgaagccg accgagaagg tcagcagctt ctccttgccg gcctcggcga gcagcgccac
      901 cagcaggctg gaatcgagcc cgcccgagag cagcacgccg accggcacgt cggcgacatc
      961 catgcgccgg cgcacggcgg tgagcaggcg ctcgtgcacg gcttcgagcc attcgtcgtc
     1021 ggacctcgcc atgaccggac gtgcggcggc gagcgtccag taggtctgct gacgctcgcg
     1081 gccatcggca aggatggtca gcgtcgtggc cggcggcagc ttgcgcacgc cggcgagcac
     1141 cgtgcgcggg gccggcacca cggcgtgcag cgtcagctgg aagtgcagcg cgaccggatc
     1201 gaccccggtg tcgatgtcgg cggcttcgag cagcgcctgc acgttcgacg cgaagcggaa
     1261 ggccgccggc gtgcgcgcgt agtacagcgg cttgatgccg agccggtcgc gggcgaggaa
     1321 cagctcctgc gtgcgcaggt tgcggatcgc gaacgcgaac atgccgatca ggcgctcgac
     1381 gcagcgctcg ccccactcgg cgtaggcctt caggatcacc tcggtgtcgc cgctcgatgc
     1441 gaacgtgtgg ccgcgcccct gcagctcggc gcgcagttcg cggtagttgt agatcgtgcc
     1501 gttgaacacg atcgcgagcc cggcggcggc atcgagcatc ggctggttcg agcgcgccga
     1561 gaggtcgatg atcgcgaggc gccggtggcc gaaggcgagc gggccgtcct gccagtgccc
     1621 ggcgctgtcc ggcccgcggc gcgcgagccg gtcggtcatg cgctgcagca ggtcggggtc
     1681 gggacgattg ccgtctaggc gcagttcgcc gcagatgccg cacatgtcgt cgccagctcc
     1741 gtatcggtat ccgagggggg cggccagcct agcacgcacc ctgcgcgcgc ggccgtcgtt
     1801 gaactgacat acccgcctgc atagactccg cggcgtgtcc tccccccgcc gaatccggga
     1861 acacgggccc ggcgcccggg ttcccattcc ctgcggtccg ccgcaacgct cgccccgccg
     1921 ttccgtagcg gtcattccct gaaaacgagc ctcaagccac aaggagacac cgaccatggc
     1981 ccatgaactg cccgccctgc cgtacgcgaa ggatgccctg cagccgcaca tctccgcgga
     2041 aacgctggag tatcactacg gcaagcatca caacgcctac gtcaccaacc tcaacaacct
     2101 gatcaagggc accgacttcg agacgctgag ccttgaggac atcgtgctga agtcctccgg
     2161 cggcatcttc aacaacgccg cgcaggtctg gaaccacacc ttctactgga actgcctgtc
     2221 gccgaacggc ggcggtgaac ccgcgggcgc gctcgccgat gcgatcaacg ccaagttcgg
     2281 ttcgttcgcg gccttcaagg aagaattcag caagtgcgcg gtcggcacct tcggctccgg
     2341 ctgggcctgg ctggtcaaga acgccgacgg ctcggtcgag ctgatgagca cctcgaacgc
     2401 cggcacgccg atgacctcgg gcaagaccgc gctgctgacc tgcgacgtct gggagcacgc
     2461 ctactacatc gactaccgca acctgcggcc gaagtacgtc gaggccttct ggaacctggt
     2521 caactgggag ttcgccgcgg cgcagtacgg cgcctgaacc cggcgggcgc cacccggcgc
     2581 cccgccaccg cctcccccat gacgggccgg tcctccggcc cgtctgcttt tgcggggccg
     2641 gcgtcaccgg ctgtcacgcc gctggcacag ccacgcgggc gcgcatgcag cgtccggtca
     2701 ttcccgcacc acggcttcag ctgcaagcca ctgatccgtc atggtcagac cagaatggca
     2761 ccagcgttgc aggggtaagt gtgttccgta ccggcaacgg acatcatcca gaccacggca
     2821 gcggtcgccc cggaaagggc ctcacagaac cgcgcccgtc accaccacac acctgggcgc
     2881 tgaccatcag ggatcgggag gagagtcgtc tcaccatgcg tacggatcac caactgctgg
     2941 aacaactgct ggaactgcac gagccgtggg aaatccgcgc cgtgcgcacc gacatccatg
     3001 gccgtcgctg cgaggtcgag gtcgggctgg ccgcgccgcg cacctggttc ggcctggtgc
     3061 gcaagccttc ggccccggcc gtcgagcgcg gcgaatggcg gcacctgaac ctcgccggct
     3121 tccgttacgt cgtgcgcgtg gtcgcgccgg cgagcgtcag cctcgagcgc tacgaatgga
     3181 ccggcgagcc ggagcagccg ttcacgcgcg cgatgtcggc gcgcctgacg cggctgctcg
     3241 ccgaaggcgt cagcctcgaa ggcatctgcc ggctgttcgg cgtcgacccg ggcgagatct
     3301 ggcggctcaa gcgcagcctc gaaacccgca gcgtgcgggt cagtccgctc gacgccgcag
     3361 gcgcggcgcc cgacaccggc gcgggtccgg ccgccggagc ggcgcccggc ctgcccgagg
     3421 ccgacgatcc ggtctggggc cggctcgccg gcggcgacat cagcatcgac atcggcgtgc
     3481 tgagcctcaa gctgctgctg acgcgcgtgc gcagccagta cgaggccatg cccgatgacg
     3541 aatcgcgacg gctgaaggcg cgcgagctgc accgtttctt cgtcaagaac ctgcgcatgc
     3601 tcggtgccga actgcagcag ctgcgcgcca gcgccctcag ggaggaagcg tgatgatgaa
     3661 ctccggtacg acgctgccgg tccgcgcacc gggcgccgac ctcggcgatc tgctcgacgt
     3721 ggacgcggtg tcggcgctga tcctcgccgg cgaacgcctg ctgctcgccg gtgacgaggc
     3781 cctgctggca cgcctgccgc gcggcacctg gatcggcggc acgatcccgt acttcgtcgc
     3841 ggccgacggc ggcaccagca gccgcgagcg gctgttctgc acgcggctgc cggccgaggt
     3901 cgaggtcgat tcgatccggc tctacgacgc ggcgagcctc gcgcacgtct gcgccgacgc
     3961 gccggccgag ggcttctcgg tgatgatcct gccggcgttc tcggaggtgc attcgctgtt
     4021 cgcgcgcaac gcgccgaact acgaggacat gtacctgaag ccgctgatcg gctggatcgc
     4081 cggcgtgcat ctcgacgacc tcggccgcgc cacgccgaag gtgttcgacg gcagccgcgg
     4141 cgagggctgc gaggagcgcg cggtcgtgct gcatgcgcgc ctgcccgagc cgcgctacgc
     4201 gcgcatcgag atcgtcaacc tgtttcgccc cggcagcggc cccggcctgc gcttcaccga
     4261 caagggcttc tcggcggcga aggtgctggt cgatggcatc gagcaggatt tcgccgcctg
     4321 gctgcgccgt cagggcaccg acacgcgcct gccgctggtc gccgactact gcggcgcgat
     4381 ggtcaacgtc agcatcaaga gcgtcgaggc cgggcgcgtc gatttctacg cgccggtgtt
     4441 cccggacatc gagtaccgca tcgccgcgcc gatcgacgac tacgtcgccc gcttcctcgc
     4501 cgcgctgccg gccgacgtgc cgccggtgac cttcagctgc aactgcatcc tgaactacct
     4561 gtactcgaac ctcgaaggcc ggcacaccgg cccgatgcgc gggccggcga ccttcggcga
     4621 gatcgcctac cagctgctga accagacgct ggtgtacctg acgatcgagg gctgacggcg
     4681 aaggcggtcc ggacacggcg cgcgggcttt tgcgcgcgcg ccgtgcggtg taccttgcgc
     4741 gccttcgcgc cgcgcagccc ccgcaggaat cccgccatgt ccgaaacccg cttcgtcgtc
     4801 gagcccggcg gccggctgac cggccgcatc cgcgtgccgg gtgacaagtc gatctcgcac
     4861 cgctcgatca tgttcggatc gctggccgat ggcgtcaccg aggtcagcgg cttcctcgaa
     4921 ggcgaggact gcctggcgac gctgaacgcc ttccgggcga tgggcgtgcg catcgacggc
     4981 cctgcggccg gtcgcgtcac catccacggc gtcggcctgc acggtctgaa gccaccggcc
     5041 gggccgctcg acatgggcaa ctccggcacc tcgatgcggc tgatgagcgg catcctgtcc
     5101 ggccaggcct tcgacacggt gctgaccggc gacgcctcgc tgtcgaagcg gccgatgaag
     5161 cgcgtgaccg cgccgctcgc gcagatgggc gcgcgcatcg aaaccaccga gcgcggcacg
     5221 gcaccgcttc acatccgcgg cgcgagcggg ctgcacggca tcgactacgt gctgccgatg
     5281 gcgagtgcgc aggtcaagtc ctgcctgctg ctcgccggcc tgtacgcgag cggcacgacg
     5341 cgcgtgaccg aaccggcacc gacgcgcgat cacaccgaac gcatgctcga aggcttcggt
     5401 tatccgctcg tgcgcgaggg cgagcgcacg ttctcggtcg gcggcggcca ccggctcggc
     5461 gcgaccgcga tcgacgtgcc ggccgacatc tcctcggcgg ccttcttcct ggtcggggcc
     5521 agcatcgcgc cgggctcgga tctcacgctc gaacacgtcg gcatcaatcc gacgcgcacc
     5581 ggcgtcatcg acatcctgcg gctgatgggc gctgacatcg aggtgcagaa cccgcgcacc
     5641 gtcggcggcg agccggtcgc cgacctgcgc gtgcgcgccg cgccgctgcg gggcatccgc
     5701 attcccgagg ccctcgtgcc gctcgcgatc gacgagttcc cggtgctgtt cgtcgccgcc
     5761 gcctgcgccg cgggcgagac cgtgctgacc ggggccgagg aactgcgcgc caaggaatcc
     5821 gaccgcatcc aggtcatggc cgacggcctc gtggcgatgg ggattgacgc gcgcccgacc
     5881 gccgacggca tcgtcatcca gggcggcgcc ttcgcgcgcg gcgccgagat cgccacgcac
     5941 cacgaccacc gcatcgcgat gagcttcacg atcgccggcc tgcgcggcgc cggcacgcac
     6001 gtgatccgcg acgccggctt cgtcgacacc tcgttcccgg gcttcgtcga gctggcgcgc
     6061 acggccggca tcgacgtgcg cgcggagcgc agcgcatgaa ggaaaccgtg ccggtcatcg
     6121 ccgtcgacgg cccgagcggc gtcggcaagg gcacgctgtg ccggagcctc gccgaagccc
     6181 tcggcttcca cctgctcgat tccggctcgc tgtaccggct gacggcgctc gccgccgccc
     6241 gcgccggcct gcgcgacgac gatgccggcg ggctcgcgca ggtggccgca acgctcgacg
     6301 tgcgcttcga cagcggtgag gacgatctcg tcgtcctgct cgccggcgag gacgtgaccg
     6361 ctgcgatccg caccgaatcg gccggcatgg cggcctcgaa ggtcgccatc gtgcccgagg
     6421 tgcgcgccgc gctgctgcag cgccagcgcg atttccgccg cgcgcccggc ctggttgccg
     6481 acggccgcga catgggcacg gtcgtgtttc ccgatgcccc gctgaagatc tttctggtcg
     6541 caagccccgc cgaacgcgcg cggcgccgac ataagcagtt gagcgagaag ggagtcgatg
     6601 ctaacctcgc cgcgctcgaa acggagatcg ccgagcgcga cgcccgtgac gcggggcgcg
     6661 ccagcgcgcc gctgaaaccg gcgccggatg cggtgatcct cgataccgat gacctcggca
     6721 tcgatgccgt ccgcgaccgc gcgctggcgc tggtcgccgg acgtctgcac gttcccgggc
     6781 agcaggaccg gtcctgaaaa accggtcagg ggtttcgcag cggcctgatt cgagccgcgg
     6841 cgaaacggca gtgaaacggt ggcgcgctga caacgtgccg acaacgcaac cgaaaacccg
     6901 aacaggccct gcctgtgaag gttcttcatt ttcttccggg ttccaacaca tgtccgaaag
     6961 ttttgccgag ctgtttgaac agagcctgtc ccagagccag atggtcccgg gttccatcgt
     7021 catgggcaag gtcgtggaca tccgcggcga ttcggtggtc gtcaacgccg gcctgaagtc
     7081 cgagggtctg atcccgctcg aacagttcct gaacgacgac ggcgtcatcg aagtcaagat
     7141 cggtgacgag gtcgaagtcg cgctcgatgc gatcgaggac ggcttcggcg aaacccgcct
     7201 gtcgcgcgag aaggccaagc gcgcccgcgt ctggagcgtg ctcgaaggcg ccttcgaagg
     7261 cgacgagatc gtgcgcggca cgatcagcgg caaggtcaag ggtggcttca cggtcgacat
     7321 cggcgagatc cgcgccttcc tgccgggttc gctggtcgac gtgcgtccgg tgcgcgacgc
     7381 cgcctacctc gaaggcaagg aactcgaatt caaggtcatc aagctcgacc gccgtcgcaa
     7441 caacgtcgtg gtctcgcgcc gcgccgtggt cgagcaggag tacagcgccg agcgcgaggc
     7501 gctgctcgaa agcctgcagg aaggccagat cgtcaagggc gtggtcaaga acctcaccga
     7561 ttacggtgcg ttcctcgacc tcggcggcat cgacggcctg ctgcacatca ccgacatggc
     7621 gtggaagcgc gtcaagcacc cgtccgaagt cgtcgccgtc ggtgacgagg tcgaggtcaa
     7681 ggtgctgaag ttcgaccgcg agcgcaaccg cgtctcgctc ggcctgaagc agctcggcga
     7741 cgatccgtgg gtcgcactcg cccgccgcta cccggtcggc acccgcctgt tcggcaaggt
     7801 cacgaacatc gccgactacg gctgcttcgt cgagatcgag gaaggcgtcg agggtctggt
     7861 ccacgtctcc gaaatggact ggaccaacaa gaacgtcaac cccggcaagg tcgtcagcat
     7921 cggcgacgaa gtcgagatca tggtgctcga catcgacgag gaccgccgcc gcatctcgct
     7981 cggcatgaag cagtgccagc cgaacccgtg ggacgagttc tgcgcgaccc acaacaaggg
     8041 cgatcgcgtc agcggcaaga tcaagtcgat caccgacttc ggcatcttca tcggtctcga
     8101 cggcggcatc gacggcctcg tgcacctgtc cgacatctcc tggaacgtcc ccggcgaaga
     8161 ggccgtgcgt gactacaaga agggccagga agtcgaggcg gtcgtgctcg ccgtggatcc
     8221 ggagcgcgag cgcatctcgc tcggcatcaa gcagcttgag aaggatccgt tctcgaactt
     8281 cgtcgccgac aacccgaagg gcagcatcgt caagggccgt gtcgcctcgg tcgatgcccg
     8341 cggcgcggtg atcgatctcg gcaacggcgt cgaaggcacg ctgcgtgtct ccgaactggc
     8401 gcgcgagcgc atcgaggatg cccgcacggt gctgaaggac ggcgaggaga tcgaggccaa
     8461 gttcatcggc gtcgatcgca agaaccgcac gatcacgctg tcgatcaagg ccaaggaata
     8521 cgccgaggaa gccgaggtgc tgcaggacta cagccgcaag tccgccaccg gcgcgacgct
     8581 cggcgacctg ctgaaggagc agatgggtcg cgaggactga tccccgcgcg agcccgtggt
     8641 ccgggacggc atcggcaccg tcccggacca ctccatggcg gcgatccgcc gctgccccgc
     8701 agaaccgccg gaaccccatt tttcgagtac tgccggaaca tcatccatga ccaaatcgga
     8761 actgatcgaa ctgctggccc gcaagcagag ccacctcagt tacaaggacg tcgaaaacgc
     8821 cgccaaggtg atgatcgaac agatgagcca gtcgctgtcg ggcggtgacc gcatcgaaat
     8881 ccgcggcttc ggcagtttct cgctgcattt ccggccgccg cgcgtcggtc gcaatcccaa
     8941 gaccggcgac gccgtgacgc tgcccggcaa gttcgtgccg cacttcaagc ccggcaagga
     9001 actgcgcgag cgcgtcaacg cctcgatcga aaacgacgat ccgcgcccga ccgcccgcat
     9061 cgccgaaccg gcctgaacac ccgtcctcgt gcggacggca tgccggtagc ggtgccgtcc
     9121 gtgctgtcct gagccaattt ccgtctgtcc ccgacgccgg gatggcgtcc gtcctgctgc
     9181 ccgtggagca ccctgatcca tgtttcgcat cgtcggcatc ctgctgttcc tgctcgtgct
     9241 cgtcgtcggc atcgaattct cggcggtcaa ctccgcgccg gtgaccgtca attacctgct
     9301 cggcacggtg tcactgccgc tggcgctggt cgtggtcgct gccttcacgg tcggcatcct
     9361 ggtcgcggtc gcggtcggca cgctggcgat ggtgccgctg cgcctgcgcg ccgcgtcgct
     9421 gcgccgtgag gtcgaggccc ggcagttcga gatcgagcgc ctgcgcgcca ggcagccggg
     9481 cacctgagcc ggaccgcatg ctcgaactgc tctggctgct gctgccggtg gcggcagcct
     9541 cgggctggtg ggccgcggca cgcagtctcg agcgcaagga gacgccgccg aacgacgtgt
     9601 tccgcggcct caactatctg ctcgatgaga agcccgatcg cgccatcgag gtcttcggcc
     9661 gcatggtcga ggccgacccc gatgccgtcg atatccagct gacgctcggc agcctgttcc
     9721 gccggcgcgg cgaggtcgac cgcgcgctgc acatccacag cgagctgctg cgccgtgcgc
     9781 agctgagtgc cggccagcgc ggccgtgcgc gcctcgaact cggtgaggac tatctgcgcg
     9841 caggcctgtt cgaccgcgcc gagcaactgt tcaccgaact gctcgaactt cccgattacc
     9901 gctcgcgggc actggtacga ctgctgtcga tctacgaaca ggagcgcgag tggcagcggg
     9961 cactcgacgc ctgcgacgag gtcgagcggg ccaccggcac gccgatgcgc atcgtggccg
    10021 cgcactactg ctgcgaactg gccgaagagg ccgtgcgtgc cagtgatcgc gccacggccc
    10081 gcagctggct cgaccgcgcc ctggtccatg acgagcgctg tgcgcgggcc gcgatcctgc
    10141 gcggccggct ggagctttcg gacggcaatc cggcgggtgc gctcgacgcc tatacgcgcg
    10201 tggtcacgcg agccccggaa tacctgcccg aagtgctcgg ccccctgcag cgctgctacg
    10261 cgcagctcca ccggcaggcc gactgggtga cactgctgcg cggcctggtc aaccagtatc
    10321 acgtcggcag tgtcaccgga gccctcgcgc aggcgctgcg cgaacgggaa ggtccggagg
    10381 ctgcgcttgt cttcctcgaa gccgaggtcg cgcagcaccc ctcgaccgag gggctgcgca
    10441 cgctgatcga tctgcgactc gccgccgagc agccggcgcg cgccggggac ctcgaactgc
    10501 tgctgcgcat cggccggcag gtgaccgatc tcggcccgcg ttaccgctgc gacaactgcg
    10561 gcttcgtcgg acgcacgctg cactggtcct gtccgggctg caagcgctgg aacaccgtca
    10621 agccgatccc cgatcgcctg cgccgcaatc cctgaagccc ccgcctgact gtcccccgcc
    10681 ggagattctg ccccgatgag caccccgtcc ccgcaggcgc gcgacccgcg cctgatcgtc
    10741 gcgctcgatt accc
//
LOCUS       DAOQIE010000114        10584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3133_length_10584_cov_7.095926, whole genome shotgun sequence.
ACCESSION   DAOQIE010000114 DAOQIE010000000
VERSION     DAOQIE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_10584_cov_7.095926"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..563
                     /locus_tag="PK144_08720"
     CDS             18..563
                     /locus_tag="PK144_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF65849.1"
                     /translation="MPGDLAMWFFILAELTVFAALFLAFAYARLWHTAEFAAGQTLID
                     RGAGAINTVLLITSSAFVVQAVACIRADDRPGCGRWLLAAIATGLGFVVFKSAGFAQA
                     FAAGVSLDGGVFPMFYLLLTGFHFLHVLLGLVILAAVWVKNRAGAYSAAGHTGVETGA
                     SYWHMVDLVWIVLFPLVYVLR"
     gene            582..836
                     /locus_tag="PK144_08725"
     CDS             582..836
                     /locus_tag="PK144_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNF65850.1"
                     /translation="MLRIWIVLMLLTAAAWAVGIKGWSGDAVAAAVLATTFIKGQLLV
                     DHYLGLACVRGPWRWLLSAWLVVVLGAVGMAFHLARGAGA"
     gene            833..1822
                     /locus_tag="PK144_08730"
     CDS             833..1822
                     /locus_tag="PK144_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF65851.1"
                     /translation="MSDEGRQRLRLWLRCGFFALFVLAPVFDVFRLDLTRGHFVLFGL
                     DWTLGIDDFTAGRIDATELSLRVVLRAFVPGLAFVLGGLWLAWKYGRLYCGWLCPHGS
                     VVETVNALMRRAGGTPSLWERRPATPRQADGSVFEPKALWWLPTALVVTGFAGLWAVT
                     LLTYLLPPAEVWGNLWHLEPTRNQALFIGVATGLFTLEFTLARHLFCRFACAAGLFQS
                     HAWVANRRALVITYQRDRAADCAGCEAVCEIACPMRLKPRTIKRRKFACTQCTACISA
                     CTTVQQHNPAGSLLAWQSGEAALAESGQRALRRIEVTGSRKVGAAADVRSEAA"
     gene            2045..2626
                     /locus_tag="PK144_08735"
     CDS             2045..2626
                     /locus_tag="PK144_08735"
                     /inference="COORDINATES: protein motif:HMM:NF023176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HNF65852.1"
                     /translation="MSEDASFLRRWAQRKADAQRPAEAAGPDIDPVSEASPAGSSPPP
                     ADASPTAADASVPLPPIESLGPDSDYRPFLAQGVDKALRTQALRKLFHQPEYNVIDPL
                     NDYIDDFTDFAPLGDVMTADLRHRLEQEAEAVAQRLQVEEPPLAGAAPSAVPADAAPA
                     AGVPAVTDAPSAGEKLISSSPEADVPPESPSRA"
     gene            2619..4250
                     /locus_tag="PK144_08740"
     CDS             2619..4250
                     /locus_tag="PK144_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF65853.1"
                     /translation="MPDPDPSSAITSADARAQALAAAAAVAVTPTSTVDFVSQGRVLI
                     LGPGTATLMAAGRLCTTLQVVVLASGPAAVPVPAGVTLLYREQRRLTLDGQLGGFFAT
                     LDADGRSFDLGERIDAQRPVFDLVLDLGDEPAFRREWGPLGYYRPLDAEALEAALAEL
                     PELCGEFEKPKFFAYDASICAHGQAGLDGCRRCIEACPAEAIESLLDRVRVNPNLCQG
                     GGACASACPSGAIVYRYPPAADTLLRLRKLLTTYRAAGGERPVLVFMDEHAGIDPLAW
                     PGRVLPVLLAETASAGMDLWLGALAYGAAAVRVLRAPDLAPKLERALDEQLGFARAIL
                     AGLGWPAEALGWLAVDGDPLTGAEMPAMPAAGYAGSEAKRERLWAAIDHLHAHAPQPA
                     EVLALPQGAPFGQILVNPETCTLCMACVSVCAPKALADGGEEPKLRFVEAACVQCGLC
                     ETACPEHAIERETRLLLPFEQRRRQRTLHEDQPHHCPQCGKAFATQSMIRTITGRLAH
                     HPMFAGDGLRRLLLCEDCRVRDLYRSEGLGQRPGP"
     gene            complement(4308..5009)
                     /locus_tag="PK144_08745"
     CDS             complement(4308..5009)
                     /locus_tag="PK144_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF65854.1"
                     /translation="MTDSRPYTIEALKLGPMDNFVYLIADRASARAAVVDPAWDVPAV
                     LARAAGLGLHITDALLTHSHQDHINGVGALLEATDARVHLHRDEAAFWGAELVRPALH
                     RGGDTLLLGSTPIEVLHTPGHTPGSVCYRVHDEVVTGDTLFVYGCGRCDLRGGDPNLM
                     HDSLCRLCRELPGGTLIRPGHDYGITPVSTMLEQMQGNPFLHFHEDQRAGFVEYRMHL
                     HDREEPYGPEPAPQG"
     gene            complement(5453..5857)
                     /locus_tag="PK144_08750"
     CDS             complement(5453..5857)
                     /locus_tag="PK144_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF65855.1"
                     /translation="MTRQIFVNLPVRDLARTRSFFESLGFEFNEKFSNDEAACLIVGD
                     GRYVMLLAENFFSTFTHKPVCDARSATEVLVCLGCDSRAEVDDLVARARAAGGSVPRD
                     AQDHGFMYAHAFEDPDGHIWELVYMAENAEAA"
     gene            complement(6070..7278)
                     /gene="nifS"
                     /locus_tag="PK144_08755"
     CDS             complement(6070..7278)
                     /gene="nifS"
                     /locus_tag="PK144_08755"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNF65856.1"
                     /translation="MNAPVYLDNNATTRVDPAVLAEMLPFFTELFGNPSSMHHYAAGV
                     AEAVQLARRQVQSLLGAKHDSEIIFTSCGTEANNTALYSALAANPGRREIITSVVEHP
                     AILAVCEHLEKNEGVTVHRIPVDAKGRLEIGAYQAALSDKVAIVSFMSANNETGTLFP
                     VEELAVMAKEVGALFHTDAVQAVGKLPMNLADSAIDMLSLSGHKLHAPKGVGALYLKR
                     GTRFRPLLRGGHQERGRRAGTENVTGIVGLGKAAELAQAAMEHENTVVRTLRDRLQTG
                     ILQSISHCFVTGDIEHRLPNTVNIAFEYIEGEAILLLLSRAGIAASSGSACTSGSLEP
                     SHVMRAMNIPYTAAHGSTRFSLSRDTTDADVDRVLAVMPEIIARLRQLSPYWGKDGPK
                     ATPDFAPAYA"
     gene            complement(7283..8206)
                     /gene="nifU"
                     /locus_tag="PK144_08760"
     CDS             complement(7283..8206)
                     /gene="nifU"
                     /locus_tag="PK144_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNF65857.1"
                     /translation="MWDYSDKVKDYFFNPVNAGVLEEANAVGDVGSISCGDALRLMLK
                     VDPETDVILDARFQTFGCGSAIASSSALTEMIKGMTLDQALKVTNQDIAAYLDGLPPE
                     KMHCSVMGAEALHAAIANYRGEEWKDDHEEGELVCKCFGVDAALIERTVRDNGLVTVE
                     QVANYTKAGGGCCSCHEKIEDILIAVNGELAATAKPAIVQQPKPRTLVQIAPLKPEPA
                     PAPAKPAMTMVRKIQLIEKVLEDIRPSLKMDGGNAELIDVDGDTVYLHLTGACMGCSS
                     SGLTIAGIQERLMLAFGKPMRVVPSKPTAEV"
     gene            complement(8212..8580)
                     /locus_tag="PK144_08765"
     CDS             complement(8212..8580)
                     /locus_tag="PK144_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF65858.1"
                     /translation="MVMLTDKAVDAVRGVLTAQTETVAGLRVLVKTGGCAGYEYALGL
                     EVEPAADDAVLAYGDDVKVFVDPVSLPLLDGVTVDFVEDITGVGFRFDNPNAVKTCGC
                     GSSFSTDANAPGGTCSSKRA"
     gene            8894..>10584
                     /gene="nifA"
                     /locus_tag="PK144_08770"
     CDS             8894..>10584
                     /gene="nifA"
                     /locus_tag="PK144_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HNF65859.1"
                     /translation="MGTQAHAGSRHHLELVTIYEISKILSRSLDLRRSLHDVLNLLAT
                     HLHVRRAMVCLSAGDGHDLQVVAETGLSPDEAKRAQFRTGEGIIGRIFKSGMPIVVPD
                     INNEPLYLHRTAERPQPGEIVAFLGVPLREGTNVVGVLSVDRRTEGEPASRFDTDLRF
                     LMMIGNLIGQTIRLHRMVEHAQENLRVENRRLATELKGKYTIENVIGRSKRMQQVFAE
                     VHQAAPGRSTVLLRGESGTGKEVIARAIHTLSNRHDGPFVKVNCAALPESLLESELFG
                     HEKGAFTGATQERKGRFEMAHGGTLFLDEIGDISPGFQAKLLRVLQEREFERVGGNRT
                     IRVDVRLITATNRNLEEAVTKGEFRADLYFRINVLSIFLPALRERREDIPLLVEHFVE
                     RVGEELGRDLRVSPEAMHVMANCHWPGNVRELENCVERMATMARGNVIRDSDLACQKN
                     LCFSSVFWKPNDHRVIPIVNGNAAASAVSSATGATPGGIAPPTPVLPVNGSSSYSSSA
                     PVASAPVVSPVPPVTVDEADFGEPELRTSRERLVSAMEQCGWVQAKAARLLGLTPR"
BASE COUNT         1591 a         3507 c         3734 g         1752 t
ORIGIN      
        1 tcgacgatcg tccgccgctg cccggcgatc tggcgatgtg gttcttcatc ctcgccgagc
       61 tgacggtgtt cgcggcgctg ttcctggcct tcgcgtatgc gcggctctgg cacacggccg
      121 agttcgcggc cgggcagaca ctgatcgatc gcggcgccgg agcgatcaat accgtgctgc
      181 tgatcacgtc gagtgccttt gtcgtgcagg cggttgcgtg catccgtgcg gatgaccgtc
      241 cgggttgcgg gcgctggctg ctggcggcga tcgcgaccgg gctcggcttc gtcgtattca
      301 agtccgcagg cttcgcgcag gccttcgcgg ccggggtttc gctcgatggc ggcgtgtttc
      361 cgatgttcta cctgctgctg accggctttc acttcctgca cgtgctgctc gggctggtga
      421 tcctcgccgc ggtgtgggtg aagaaccgcg ccggggcgta ttcggcggcc ggacacaccg
      481 gggtcgagac cggggcgtcg tactggcaca tggtcgatct ggtctggatc gtcctgttcc
      541 cgctggtcta cgtgctgcgc tgaggcgggg aggatatcgg gatgctgcgt atctggatcg
      601 tgctgatgct gctgaccgct gccgcgtggg cggtcgggat caagggctgg agtggcgatg
      661 cggtggcggc cgccgtgctg gcgacgacct tcatcaaggg gcagctgctc gtcgatcact
      721 acctgggact cgcctgcgtg cgcggaccgt ggcgctggct gctgtcggcg tggctcgtcg
      781 tcgtgctcgg cgcggtcggc atggcgtttc atctggcacg gggggcaggg gcatgagcga
      841 cgagggtcgt cagcggctgc ggctttggct gcgctgcggt ttcttcgcgc tgttcgtgct
      901 ggcgccggtg ttcgatgtgt tccggctcga tctgacgcgc gggcacttcg tgctgttcgg
      961 gctggactgg acgctcggca tcgacgactt cacggccggg cgcatcgatg cgacggaact
     1021 gtcgctgcgc gtcgtgctgc gcgccttcgt gccggggctc gcgttcgtgc tcggcggtct
     1081 ctggctggcg tggaagtacg ggcggctgta ctgcggctgg ctgtgtccac atgggtcggt
     1141 ggtcgagacg gtcaacgcgc tgatgcgtcg cgccggcggc acgccgagtc tctgggaaag
     1201 acgcccggca acgccgcgac aggccgatgg ttcggtgttc gagccgaagg cgctctggtg
     1261 gctgccgacc gcgctcgtcg tgaccggctt cgccggcctc tgggccgtga cgctgctgac
     1321 ctatctgctg ccgccggccg aggtctgggg caatctgtgg cacctcgaac cgacgcgcaa
     1381 tcaggcgctg ttcatcggcg tggcgaccgg gctgttcacg ctcgaattca cgctggcgcg
     1441 gcatctgttc tgccgcttcg cctgcgcggc cggactgttc cagagtcatg cgtgggtggc
     1501 gaatcgcagg gcgctggtga tcacctatca gcgcgatcgc gccgccgatt gcgccggctg
     1561 cgaggcggtc tgcgagattg cctgtccgat gcgcctgaag ccgcgcacga tcaagcgcag
     1621 gaagtttgcc tgcacgcagt gcacggcctg catcagtgcc tgcacgacgg tgcagcagca
     1681 caaccccgcc ggcagcctgc tcgcgtggca aagcggcgag gcggcgcttg ccgaatccgg
     1741 gcagcgggcc ttgcggcgca ttgaagtgac cggcagccgc aaggtgggcg cggcggcgga
     1801 cgtgcgatcc gaagcggcct gaagcggaaa aggccggaca gccggcgtgc agccggtccg
     1861 tgccggtgcc ggggctttct gctgcgcagc gaagctttgg cggggccggg cgaaaggctt
     1921 gatccgcaag ggatcgatgc ggaagcgcgc atccggcgcc ttggtccgca ggcggcttgc
     1981 agatcggccc ggcagttcct acgctgccgg ccccccgtgc tgctgcgagt cgcaggccag
     2041 gaccatgagc gaagatgcct ccttcctgcg ccgctgggcg cagcgcaagg ccgatgcgca
     2101 acgccccgcc gaagcggccg gcccggacat cgatcccgtg tccgaagcgt cgccggccgg
     2161 gtcatcgccg ccgcctgcgg acgcaagtcc gacggctgcc gatgcttccg tgccgctgcc
     2221 gccgatcgaa tcgctcggac cggattccga ctaccggccc ttcctcgcgc agggcgtcga
     2281 caaggcgctg cgcacgcaag ccctgcgcaa gctgtttcat cagcccgaat acaacgtcat
     2341 cgatccgctc aacgattaca tcgatgactt caccgacttc gcgccgctcg gtgacgtgat
     2401 gaccgccgac ctgcgccatc ggctcgaaca ggaggccgag gctgtcgcgc agcggctgca
     2461 ggtcgaagaa ccgccgctgg ccggcgcggc cccgtccgcg gtccctgccg atgccgcgcc
     2521 ggcagcaggc gtgccggccg tgaccgatgc tccgtccgcc ggagagaagc tgatttcctc
     2581 aagtcctgag gccgatgtgc cgccggagag cccttcccgt gcctgatccc gatccttcct
     2641 ccgcgatcac gagcgcggat gcgcgcgcgc aggcgctggc cgcggccgcc gccgtggcgg
     2701 tgacgccgac ctcgaccgtc gatttcgtct cgcagggccg ggtgctgatc ctcgggccgg
     2761 gtacggcgac gctgatggcg gccgggcggc tctgcacgac gctgcaggtc gtcgtgctcg
     2821 cgtccgggcc ggccgccgtg ccggtgccgg ccggtgtgac gctgctgtat cgcgagcagc
     2881 gccggctgac gctcgatggc cagctcggcg gcttcttcgc gacgctcgat gcggacgggc
     2941 gttcgttcga tctcggcgaa cgcatcgatg cgcagcggcc ggtgttcgat ctcgtgctcg
     3001 atctcggcga cgagccggcg ttcaggcgcg aatggggacc gctcggctat taccggccgc
     3061 tggatgccga agccctcgaa gccgcgctcg ccgagctgcc ggagctgtgc ggcgagttcg
     3121 agaagccgaa attcttcgcc tacgacgcct cgatctgcgc gcacggccag gccggactcg
     3181 atggctgccg gcgctgcatc gaagcctgcc cggccgaggc gatcgaatcg ctgctcgatc
     3241 gcgtgcgtgt caatccgaac ctctgtcagg gcggtggcgc ctgcgcgagc gcctgcccga
     3301 gcggtgcgat cgtctaccgc tatccgccgg cggccgacac gctgctgcgc ctgcgcaagc
     3361 tgctgacgac gtaccgcgcc gccggcggcg agcggccggt gctggtgttc atggacgagc
     3421 acgccggcat cgatccgctg gcctggccgg ggcgcgtgct gccggtgctg ctcgccgaga
     3481 ccgcctcggc cggcatggac ctctggctcg gggcgctggc gtacggcgcg gcagcggtgc
     3541 gcgtgctgcg cgcaccggat ctggcgccga agctcgaacg cgcgctcgat gagcagctcg
     3601 ggtttgcgcg cgcgatcctc gccggactcg gctggccggc cgaggcactc ggctggctgg
     3661 ccgtggacgg cgatccgctg acaggtgccg aaatgccggc catgccggcg gcgggctatg
     3721 ccggcagcga ggccaagcgc gaacggctgt gggcggcgat cgatcatctg catgcgcacg
     3781 cgccgcagcc ggccgaggtg ctggccctgc cgcagggcgc accgttcggg cagatcctcg
     3841 tcaatcccga aacctgcacg ctgtgcatgg cctgtgtgtc ggtgtgcgcg ccgaaggcgc
     3901 tcgccgatgg cggggaggaa ccgaagctgc gcttcgtcga ggcggcctgc gtgcagtgcg
     3961 ggctgtgcga gaccgcctgt ccggagcatg cgatcgagcg cgagacccgg ctgctgctgc
     4021 cgtttgaaca gcgccgccgt cagcgcacgc tgcatgagga tcagccgcac cactgcccgc
     4081 aatgcggcaa ggccttcgcg acgcaatcga tgatccgcac gatcaccggg cggctcgccc
     4141 atcacccgat gttcgccggc gacggcctgc gccggctgct gctctgcgag gactgccgcg
     4201 tgcgcgacct gtaccgcagc gagggactgg ggcagcggcc gggaccctga gggcaggcac
     4261 cgcaggcgca gcctgccggc cgggcgtttg cgtgcagccg cgagccttca gccttgcggc
     4321 gccggttccg gcccgtaggg ctcctcgcga tcgtgcaggt gcatgcggta ttcgacgaaa
     4381 ccggcgcgct gatcctcatg aaaatgcagg aagggattgc cctgcatctg ttcgagcatc
     4441 gtcgagaccg gcgtgatgcc gtaatcatgg ccggggcgga tcagcgtgcc gccgggcagt
     4501 tcgcggcaga gccggcagag cgaatcgtgc atgaggttcg ggtcgccgcc gcgcaggtcg
     4561 cagcgtccgc agccgtacac gaacagcgtg tcgccggtga cgacctcgtc atggacgcgg
     4621 tagcagaccg aaccgggcgt gtggccgggg gtgtgcagca cctcgatcgg ggtggagccg
     4681 agcagcagcg tgtcgccgcc gcggtgcagg gccggacgca cgagctccgc accccagaac
     4741 gccgcctcgt cgcgatgcag atgcacccgg gcgtcggtgg cttcgagcag ggctccgacg
     4801 ccgttgatgt gatcctggtg gctgtgcgtc agcagcgcgt cggtgatgtg caggccgagt
     4861 ccggccgcgc gcgccagcac ggccggcacg tcccaggccg gatcgacgac ggcggcgcgg
     4921 gcgctggcgc gatcggcgat caggtagacg aagttgtcca tcggcccgag cttcagggct
     4981 tcgatcgtgt acgggcggga gtcggtcacg gggcttctcc ggtgggcgag gacggcatgg
     5041 ctgcgccggc agggcggatg cacgacggaa aagtgcatcg gctgcggggt ttcaccggcg
     5101 cttgcgcatg ccgctgcaag catggctcag cctgccggcg atgccggcgg gccggatcag
     5161 caggcgatcc ggccgcggcg ggcggtcatg cacccggcag cacagccgga gtccggaaga
     5221 cgggcataaa aaaacgcggt cgggcggggg gcgaacgacc gcgtaaacag aggaggagga
     5281 gaagcgacat ccacgaagat gtcgcgttgc agcatagccg agtattttgc ttgggtaagt
     5341 tgatcatgct gcatggcagc aatacgctcg ggtatgcacg gatacggcgg tttgcgcatg
     5401 gcattgccgg cggcgatctg cagggtccga ggaggccggt ccgcccaccg ggtcaggcgg
     5461 cctcggcgtt ctcggccatg tacaccagct cccagatgtg gccgtccggg tcctcgaagg
     5521 cgtgcgcata catgaaaccg tggtcctggg cgtcgcgcgg cacgctgccg ccggcggcgc
     5581 gggcgcgggc aaccagatcg tcgacctcgg cgcggctgtc gcagcccagg cagaccagca
     5641 cctcggtggc gctgcgggca tcgcagaccg gcttgtgcgt gaaggtcgag aagaagttct
     5701 cggcgagcag catcacgtag cggccatcgc cgacgatcag gcaggcggcc tcgtcgttgg
     5761 agaatttttc gttgaattcg aagccgagcg attcgaagaa gctgcgggtg cgcgcgagat
     5821 cgcgcaccgg caggttgacg aagatctggc gcgtcatgcc tgtttcctcc tgcactgtgg
     5881 gggtgtccga cagcgtagac ggaggaagcc ggggcgcgtt ccggccccgg gtgacggtgc
     5941 ggacaggtgc cgccgcgcca aaaaagacgg ccgtaccaga tcggtacggc cgagcccgtg
     6001 cggggccatc aggatgggaa tcggtatcgg gccctgcagg gggccggtgt gagagtcccg
     6061 ggccgatgct caggcgtagg cgggtgcgaa gtccggcgtg gccttcggac cgtccttgcc
     6121 ccagtacggc gagagctggc gcagccgggc gatgatctcg ggcatcaccg ccagcacgcg
     6181 gtcgacatcg gcgtcggtgg tgtcgcgcga cagcgagaag cgcgtcgaac cgtgcgcggc
     6241 cgtgtacggg atgttcatcg cccgcatcac gtgcgagggc tccagcgaac ccgaggtgca
     6301 ggccgagccg ctcgatgcag cgatgccggc acgcgacagc agcagcagga tcgcctcacc
     6361 ctcgatgtac tcgaaggcga tgttgaccgt gttcggcagc cggtgctcga tgtcgccggt
     6421 cacgaagcag tgcgagatcg actgcaggat gccggtctgc agccggtcac gcagcgtgcg
     6481 cacgacggtg ttctcgtgct ccatcgccgc ctgtgcgagc tctgccgcct tgccgaggcc
     6541 gacgatgccg gtgacgttct cggtgccggc ccggcggccg cgctcctgat gcccgccgcg
     6601 cagcagcggc cggaagcgcg tgccgcgctt gaggtacagc gcgccgacgc ccttcggcgc
     6661 gtgcagcttg tgaccggaca gcgacagcat gtcgatcgcc gaatcggcga ggttcatcgg
     6721 cagcttgccg accgcctgca cggcgtcggt gtggaacagc gcaccgactt ccttggccat
     6781 gaccgcgagt tcctcgaccg ggaacagcgt gccggtctcg ttgttcgcgc tcatgaacga
     6841 cacgatcgcg accttgtccg acagcgccgc ctggtacgcc ccgatctcca gccggccctt
     6901 cgcgtcgacc gggatgcgat gcacggtcac gccctcgttc ttttcgaggt gctcgcagac
     6961 ggcgaggatg gccggatgct cgacgaccga ggtgatgatc tcgcgccggc cggggttggc
     7021 ggcgagcgcc gagtacagcg cggtgttgtt ggcctcggtg ccgcaggacg tgaagatgat
     7081 ctccgagtcg tgcttcgcgc cgagcagcga ctgcacctga cggcgcgcga gctgcacggc
     7141 ctcggcgacg ccggcggcgt agtggtgcat cgacgacggg ttgccgaaca gctcggtgaa
     7201 gaacggcagc atctcggcca gcacggccgg atcgacgcgg gtcgtggcgt tgttgtcgag
     7261 gtagaccggg gcgttcatgg cttcagacct ccgcggtcgg cttgctcggc accacgcgca
     7321 tcggcttgcc gaaggcaagc atcagacgct cctggatgcc ggcgatggtc aggcccgagg
     7381 aactgcagcc catgcaggcg ccggtcaggt gcagatagac ggtgtcgccg tcgacatcga
     7441 tcagctcggc gttgccgccg tccatcttca gcgacgggcg gatgtcctcc agcaccttct
     7501 cgatgagctg gatcttcctg accatcgtca tcgccggctt ggccggggcc ggggccggtt
     7561 cgggcttcag cggcgcgatc tgcaccagcg tgcgcggctt cggctgctgc acgatcgccg
     7621 gcttcgcggt cgcggccagc tcgccgttga cggcgatcag gatgtcctcg atcttctcgt
     7681 ggcaggagca gcagccgccg ccggccttgg tgtagttcgc gacctgctcg acggtgacga
     7741 gaccgttgtc gcgcaccgtg cgctcgatca gcgccgcatc gacgccgaag cacttgcaga
     7801 ccagctcgcc ttcctcgtgg tcatccttcc actcctcgcc gcggtaattc gcgatcgcgg
     7861 cgtgcagggc ctcggcgccc atgaccgagc agtgcatctt ctcgggtggc aggccgtcga
     7921 ggtaggcggc gatgtcctgg ttcgtgacct tcagcgcctg atcgagcgtc atgcccttga
     7981 tcatctcggt cagcgccgag ctggaggcga tcgccgaacc gcagccgaaa gtctggaagc
     8041 gcgcatcgag gatgacgtcg gtctcgggat cgaccttcag catcaggcgc agcgcatcgc
     8101 cgcaggagat cgaaccgacg tcgccgacgg cattggcttc ttcgagcacg ccggcgttga
     8161 ccgggttgaa gaagtaatcc ttgaccttgt ccgagtagtc ccacatggcc gtcacgccct
     8221 cttgctggag caggtgccgc cgggcgcgtt ggcgtccgtc gagaaggaag agccgcagcc
     8281 gcaggtcttc acggcattgg ggttgtcgaa gcggaagccg acgccggtga tgtcctcgac
     8341 gaagtcgacc gtcacgccat cgagcagcgg caggctgacc ggatcgacga acaccttcac
     8401 gtcatcgcca taggcgagca ccgcatcatc ggcggccggc tcgacttcga ggccgagcgc
     8461 gtattcgtag ccggcgcagc cgccggtctt cacgagcacc cgcaggccgg ccaccgtttc
     8521 cgtctgggcg gtcagcacgc cacgcacggc gtccaccgct ttgtccgtca gcatcaccat
     8581 tgttcggtac ctcgcagtac tcgggttcgg gcagggttgc ggggcttcga gcaaccgccg
     8641 tgccatgatc tgcgtcagct tccaagggat tgatgcggca gaagaagcgg cgaaataccc
     8701 acagaacgtc cggtctggtg caattgtcgg attccctaca tgcgacatgc gccatgcagg
     8761 tgcagtgtcg ggtgtcggct acccgacgat tacggtcgaa ctacttacaa atcagacagg
     8821 tgtggtgtct ggctccggta ttgcattgat cctgtgcatg gttttcacag gattcccccc
     8881 ggaggccgat gacatgggaa cccaagcaca cgccggcagc cgtcaccatc tggaactggt
     8941 gacgatctac gagatcagca agatcctttc ccgctcgctg gatctgcgcc gctcgctgca
     9001 cgacgtgctg aacctgctgg caacacacct gcacgtgcgc cgcgcgatgg tctgcctcag
     9061 tgccggcgac ggtcatgacc tgcaggtcgt cgccgaaacc ggcctgtcgc cggacgaggc
     9121 gaagcgcgcg cagttccgca ccggcgaagg catcatcggc cgcatcttca agagcggcat
     9181 gccgatcgtc gttcccgaca tcaacaatga accgctgtac ctgcaccgca ccgccgaacg
     9241 gccgcagccc ggcgagatcg tcgcctttct cggcgtgccg ctgcgcgaag gcaccaacgt
     9301 cgtcggcgtg ctgagcgtcg accggcgcac cgaaggcgaa ccggccagcc gcttcgacac
     9361 cgacctgcgc ttcctgatga tgatcggcaa cctgatcggc cagaccatcc ggctgcaccg
     9421 catggtcgag cacgcccagg agaacctgcg tgtcgagaac cggcgcctcg ccaccgagct
     9481 gaagggcaag tacacgatcg agaacgtcat cggccgcagc aagcgcatgc agcaggtgtt
     9541 cgccgaggtc caccaggccg cacccgggcg ctcgaccgtg ctgctgcgcg gcgaaagcgg
     9601 caccggcaag gaagtcatcg cccgcgccat ccacacgctc agcaaccgcc acgacgggcc
     9661 gttcgtgaag gtcaactgcg cggccctgcc cgaatcgctg ctcgaatccg aactgttcgg
     9721 ccacgagaag ggcgccttca ccggcgccac gcaggagcgc aagggccgct tcgagatggc
     9781 ccacggcggc acgctgttcc tcgacgagat cggcgacatc tcgccggggt ttcaggccaa
     9841 gctgctgcgc gtgctgcagg agcgtgaatt cgagcgcgtc ggcggcaacc gcacgatccg
     9901 cgtcgacgtg cgcctgatca ccgccaccaa ccgcaacctc gaagaagccg tcaccaaggg
     9961 cgaattccgc gccgacctct atttccgcat caacgtcctc agcatcttcc tgccggcgct
    10021 gcgcgaacgg cgcgaggaca tcccgctgct ggtcgagcat ttcgtcgaac gcgtcggcga
    10081 ggaactcggc cgggacctgc gcgtcagccc cgaggccatg cacgtgatgg ccaactgtca
    10141 ctggccgggc aatgtccgcg agctggaaaa ctgcgtcgag cgcatggcga cgatggcgcg
    10201 cggcaacgtc atccgcgaca gcgacctcgc ctgccagaag aacctgtgtt tctccagcgt
    10261 gttctggaaa ccgaacgatc accgcgtgat cccgatcgtc aacggcaacg ccgccgccag
    10321 cgccgtctct tcggccaccg gcgccacccc cggcggcatc gccccgccga ctccggtcct
    10381 gccggtcaac ggcagcagca gctacagcag cagcgctccg gttgcatcgg ctccggttgt
    10441 ttccccggtg ccgccggtga cggtcgacga agcggatttc ggtgaaccgg aactgcgcac
    10501 ctcgcgcgaa cggctggtat cggcaatgga acagtgcggc tgggtccagg ccaaggccgc
    10561 ccgcctgctc gggctgacac cgcg
//
LOCUS       DAOQIE010000115        10567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3142_length_10567_cov_7.641933, whole genome shotgun sequence.
ACCESSION   DAOQIE010000115 DAOQIE010000000
VERSION     DAOQIE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_10567_cov_7.641933"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK144_08775"
     CDS             complement(<1..217)
                     /locus_tag="PK144_08775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261929.1"
                     /note="The point of entry for the majority of electrons
                     that traverse the respiratory chain eventually resulting
                     in the reduction of oxygen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNF65860.1"
                     /translation="MGIEGLLEKGVVTTTADKLINWARTGSLWPMTFGLACCAVEMMH
                     AGASRYDLDRFGIVFRPSPRQSDVMIVA"
     gene            complement(208..564)
                     /locus_tag="PK144_08780"
     CDS             complement(208..564)
                     /locus_tag="PK144_08780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNF65861.1"
                     /translation="MLENYLPILVFVLIGVVVGGVAIAAGMVLGPNHPDSEKLSPYEC
                     GFEAFEDSRMKFDVRYYLVAILFIIFDLEIAFLFPWAVVLDQIGGFGFVAMAVFLGIL
                     VIGFIYEWKKGALEWE"
     gene            complement(780..864)
                     /locus_tag="PK144_08785"
     tRNA            complement(780..864)
                     /locus_tag="PK144_08785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(828..830),aa:Leu,seq:gag)
     gene            complement(895..1221)
                     /gene="secG"
                     /locus_tag="PK144_08790"
     CDS             complement(895..1221)
                     /gene="secG"
                     /locus_tag="PK144_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNF65862.1"
                     /translation="MYTVILFVHVLIAIALIVLVLLQQGKGADAGAAFGSGASATVFG
                     ARGSANFLSKTTAMLATGFFVTSLVLAYFASATSQPVSVVQPVPAQQLPSGPADVPSL
                     PAQPGK"
     gene            complement(1234..1980)
                     /gene="tpiA"
                     /locus_tag="PK144_08795"
     CDS             complement(1234..1980)
                     /gene="tpiA"
                     /locus_tag="PK144_08795"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNF65863.1"
                     /translation="MRRRLVAGNWKMNGSVAAVRDWCATVVQAAAEAPVDIALFPPYP
                     LLAQAMQSLAGTNVQLGAQDVSMHASGAYTGEVSAAMLIEAGCRQVLVGHSERRSYHG
                     ETDEQVAAKFAVALDAGLVPVLCIGETLAQREGGETDAVVGRQLRAVLERVGGDGLAK
                     GVIAYEPVWAIGTGRTASPMQAQAVHENIRGILRDANATMAGSMRILYGGSVNGDNAA
                     QLFEMPDIDGALVGGASLNAREFLRIVRTA"
     gene            complement(2039..3376)
                     /gene="glmM"
                     /locus_tag="PK144_08800"
     CDS             complement(2039..3376)
                     /gene="glmM"
                     /locus_tag="PK144_08800"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNF65864.1"
                     /translation="MRYFGTDGIRGRVGEAPVTADFMLKLGWAAGKVLAGAGAQKVLI
                     GKDTRISGYMFESALEAGLSAAGVNIALLGPMPTPAIAYLTRTLRAAAGIVISASHNP
                     YWDNGIKFFARDGRKLPDEIEAAIELELDRPMTTAGPSELGKAERIIDAAGRYIEFCK
                     STVPAALDLRGLHIVIDCANGATYHTAPNVFSEIGARVTVLAASPDGLNINHECGSTR
                     PAALQKAVLEAGADLGIAFDGDGDRVIMVDRHGAVVDGDELLYIIACDRQRMHGLRND
                     AVVGTQMSNLGLELGLRELGIALYRSRVGDRFVMELLSEHGLCVGGESSGHIICLDRT
                     TTGDGIVSALQVLAAMTATGRDLRELLAGMHKCPQVMVNVPVRRRIDWGSCTPVVEAV
                     RATESDLAGRGRVLLRPSGTEPVVRVMVEGDDAARVRRLAHQLAEIVAASAAD"
     gene            complement(3479..4258)
                     /gene="folP"
                     /locus_tag="PK144_08805"
     CDS             complement(3479..4258)
                     /gene="folP"
                     /locus_tag="PK144_08805"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNF65865.1"
                     /translation="MGVLNVTPDSFSDGGRWIEPAAALEHAYAMVEAGAALIDIGGES
                     TRPGAAEVSEAEELRRVIPLVEHLSAGLAVPVSVDTSKPAVMRAAIAAGAGLINDVRA
                     LRACGALEVVADSQAAVCLMHMQGEPRTMQQAPAYADVVAEVREFLLARAAACLAAGI
                     GSQRIVLDPGFGFGKTLTHNLSLLRHLDALTGGQWPVLVGLSRKSMIGAVLDLPLERR
                     VTGSVAAALLAAAAGARIVRVHDVRETVEALGILRAVRAAP"
     gene            complement(4324..6240)
                     /gene="ftsH"
                     /locus_tag="PK144_08810"
     CDS             complement(4324..6240)
                     /gene="ftsH"
                     /locus_tag="PK144_08810"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNF65866.1"
                     /translation="MVKNILLWVVIAVVLMSVFNSFGPRTSPSQQLTYSQFLEEVKQG
                     RVAHVSIDGRSISGRTSTGEKFMTYSPETDNKAMIGTLLDNRVDVEGRPPEQQSVWMQ
                     IFISWFPMLLLIGVWVFFLRQMQGGAGGRGAMSFGKSRARMMSEDQIKVTFADVAGCD
                     EAKEEVGELVEFLRDPGKFQRLGGKIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQILK
                     VHMRKVPVGGDVRPSLIARGTPGFSGADLANLVNEAALFAARANKREVGMEEFEKAKD
                     KIMMGAERKSMVMSESEKKLTAYHEAGHAIVGRTVPDHDPVYKVSIIPRGRALGVTMF
                     LPEQDRYSYSRQRLESQISSLFGGRIAEVLIFGSDSVTTGASNDIERATEIARNMVTK
                     WGLSDRLGPLTYSEDDGEVFLGRSVTRHKNVSDETAHVIDEEIRSIIDRNYQRAEQIL
                     RDHMNALHKMSEALIKYETIDSDQIDDILAGREPRPPQDYADVEPPAAGPGASASNGG
                     KGDAGPIGGPAQQH"
     gene            complement(6314..6934)
                     /gene="rlmE"
                     /locus_tag="PK144_08815"
     CDS             complement(6314..6934)
                     /gene="rlmE"
                     /locus_tag="PK144_08815"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNF65867.1"
                     /translation="MARSRSSHRWLKEHFDDEFVRRAQAEGWRSRAVYKLQEINERDR
                     LLKPGTTLVDLGAAPGGWSQYAARALGERGRVLALDILPMDALAGVDFIQGDFREETV
                     FAQLLATLAGTPVDLVISDMAPNTSGLKAVDQPKQMYLVELALDFARQTLRPGGDFLV
                     KVFQGEGFDALLKDLRADFGSVATRKPKASRARSAELYLLARNFRS"
     gene            complement(6924..7190)
                     /locus_tag="PK144_08820"
     CDS             complement(6924..7190)
                     /locus_tag="PK144_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65868.1"
                     /translation="MSEEKGARSVARGSLSVAASPLNGVKFELGVILLVGLLLVPVVG
                     RVFAEPLWQLLALAAYGVAGALWLVLRARRVLGEQARRSAGDGA"
     gene            7208..7501
                     /gene="yhbY"
                     /locus_tag="PK144_08825"
     CDS             7208..7501
                     /gene="yhbY"
                     /locus_tag="PK144_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNF65869.1"
                     /translation="MLKQSQKRHLKALAHPLKPVVIVGANGVTPAVLDEVRLALEHHE
                     LIKVRVNAGDREQREALIADIVEATGCELVQRIGHIAAFFRRNPDKPRITLPA"
     gene            complement(7485..7964)
                     /locus_tag="PK144_08830"
     CDS             complement(7485..7964)
                     /locus_tag="PK144_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNF65870.1"
                     /translation="MSSPVRARLRDWGPVALEQLLTVVWLGSLWTVGYLVAPVLFATL
                     PERALAGQVAGRLFTLVAWLGLGCGAALLALALWRWRRAQASLTLVLLPALMLLLVLT
                     GEFGLQPRMAELKAVGESGGAMFARLHGIAALLYLAESLLGLGWLVLRVRETLRRAA"
     gene            8396..9181
                     /locus_tag="PK144_08835"
     CDS             8396..9181
                     /locus_tag="PK144_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF65871.1"
                     /translation="MIARPAPLRPAPAALPAALLSSRPPALRRGLRTGGTFAAALALH
                     VGALGHWTTPETLPQPLPMAPLVVDLIEAVPPPDPLAAAPAETAPPPEPEAPPPEPPP
                     PPEPLPQAITAPPKPPLPAPPKPKPKPVVQPPKPKPTPRPPAPNAARSETPPSPLPPA
                     PVAAAPSTTAANFSAAYLRNPPPRYPPDSRSAGEAGRVLLRVRVTAEGRAESVEIAAS
                     SGHARLDDAALAAVRRWRFVPAQQAGAPVAATVTVPILFKLES"
     gene            9244..9879
                     /locus_tag="PK144_08840"
                     /pseudo
     CDS             9244..9879
                     /locus_tag="PK144_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
     gene            10052..10477
                     /locus_tag="PK144_08845"
     CDS             10052..10477
                     /locus_tag="PK144_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF65872.1"
                     /translation="MAFGSLGSSHSRPNAEINTTPLVDVMLVLLVIFILTAPLFTHTV
                     KLDLPRAAHRTDPQPPPAVTLSLDAAGALFWDNAALAESELAPRLAALAAQQPQPELH
                     LRADRETRYERIAQLMAAARNAGVNRIGFVTDPQPAATH"
BASE COUNT         1846 a         3840 c         3311 g         1570 t
ORIGIN      
        1 cggccacgat catgacgtcg gactggcgcg gactcggccg gaacacgatg ccgaatcgat
       61 cgaggtcgta acgggaagcg ccggcgtgca tcatttcgac cgcacagcag gcaaggccga
      121 aagtcatcgg ccagagcgaa ccggtacgcg cccagttgat cagcttgtcg gccgtggtgg
      181 tgacaacgcc tttttcaaga agcccttcta ttcccattcc agtgccccct tcttccactc
      241 gtagatgaag ccgatgacga gaatgcccag gaacacggcc atcgccacga aaccaaagcc
      301 accgatctga tcgagcacga cagcccacgg gaacaggaag gcgatttcga gatcgaagat
      361 gatgaacagg atggccacga ggtaatagcg gacatcgaac ttcattcgcg aatcttcgaa
      421 ggcctcgaag ccgcactcgt aaggcgagag cttttcactg tccggatgat tcggacccag
      481 caccatgccg gcagcaatgg ccacgccacc gacgacgaca ccgatcagca cgaacacgag
      541 aatggggagg taattttcca gcatcaacgt cgttccttct gtgcgaggac tgtctaggaa
      601 gtgatgcttc tgtggcaaac cccgctgcat cgggacatgc caaagcggcg gaagtctagg
      661 ttcaccccgc cggcaagtca agaaatccag ggcgggttta atttcacaat cagagactta
      721 cccgagcaaa acacttcagt aataaaaaag gcgctcttgc gagcgcccgg atacatcaat
      781 ggtgccgacg cccggactcg aaccgggacg gcgttagcca ctaccacctc aagatagcgt
      841 gtctaccaat tccaccacgt cggcatcaaa ctgaaaatca cggcatgccg gtcattactt
      901 gcctggctgc gccggcaggc ttggcacatc agccggcccc gaaggcagct gctgcgccgg
      961 caccggctgc acgacactga ccggctgcga ggtcgcactc gcgaaatacg ccagcaccag
     1021 actggtaacg aagaaccccg tagccagcat ggctgtcgtc ttgctcagaa agttcgccga
     1081 accgcgcgcg ccgaatacgg ttgccgaagc accacttcca aacgcagcac cggcatcggc
     1141 acccttaccc tgctgtaata ataccagcac gatcagggca atggcaatca gcacatgaac
     1201 aaacaggatg accgtataca tttcgaaacc gaatcaggcg gttcgcacaa tgcgcagaaa
     1261 ctcgcgggca ttcagtgaag ccccaccaac cagcgcaccg tcgatatccg gcatttcgaa
     1321 caattgggcg gcattgtcac cattgacact gccgccgtac agaatcctca tggagcctgc
     1381 catcgtagca tttgcatccc gcaggattcc acggatattt tcgtgaacgg cctgagcctg
     1441 catcggcgaa gccgtcctgc cggtaccgat tgcccagacc ggctcgtagg caatgacacc
     1501 cttcgccaga ccgtcaccac cgactcgctc cagcaccgcc cgcaactggc gaccgacgac
     1561 tgcatcggtt tcaccgccct cgcgctgcgc cagcgtttcg ccgatacaga gcaccggcac
     1621 cagcccggca tccagcgcca cggcaaactt ggcggcaacc tgctcatcgg tttcgccgtg
     1681 atagctgcga cgctccgaat gcccgaccag cacctgccgg caacccgcct cgatcagcat
     1741 cgccgccgac acttcaccgg tgtacgcacc cgacgcatgc atcgagacat cctgcgcacc
     1801 gagctgcacg ttcgtcccgg ccagcgactg catggcctga gcgagcagcg ggtacggagg
     1861 aaacagtgcg atgtcgaccg gagcctctgc ggccgcctgc acgacggtcg cgcaccagtc
     1921 acgcaccgcc gccaccgagc cgttcatttt ccagttaccg gcgaccaatc gtctgcgcat
     1981 ggactccccc cgcaaggccg cggactctag ccataacctt accggaaagc aacctgactc
     2041 agtcggccgc gctcgcggcg acgatctcgg cgagctgatg cgcgagccgg cgtacccggg
     2101 cggcatcgtc gccttcgacc atgacgcgca cgaccggctc ggtaccggaa ggccgcagca
     2161 gcacgcgccc gcgtccggcc agatcggatt cggtcgcgcg cacggcttcg acgaccggcg
     2221 tgcagctgcc ccagtcgatg cgtcgtcgca ccggcacgtt caccatgacc tgcgggcact
     2281 tgtgcatgcc tgccagcagc tcgcgcaggt cgcgaccggt ggccgtcatc gctgccagca
     2341 cctgcagggc cgaaacgatg ccgtcgccgg tcgtggtgcg atccagacag atgatgtgtc
     2401 cggagctttc accaccgacg cacagaccgt gctcgctgag cagctccatc acgaagcggt
     2461 cgccgaccct ggaacggtac aaggcaatgc cgagttcgcg caggccgagt tcgagcccca
     2521 ggttgctcat ctgcgtgccg acgaccgcat cgttgcgcag gccatgcatg cgctggcgat
     2581 cacaggcgat gatgtacagc agttcatccc catcgacgac cgcaccgtgg cggtcgacca
     2641 tgatcacgcg gtcgccatcg ccatcgaatg cgatgccgag atcggccccg gcttcgagca
     2701 ccgccttctg cagcgccgcc ggccgggtcg atccgcactc atgattgatg ttcaggccgt
     2761 ccggcgaggc ggccagcacc gtgacccggg caccgatctc ggaaaacacg ttcggcgcgg
     2821 tgtggtaagt cgcgccgttg gcgcagtcga tgacgatgtg cagtccacgc agatccagcg
     2881 cggctggcac cgtgctcttg cagaattcga tgtagcggcc ggcggcatcg atgatgcgct
     2941 cggccttgcc gagttccgaa ggcccggccg tcgtcatcgg ccgatcgagt tcgagctcga
     3001 tggcagcctc gatctcgtcg gggagcttgc gaccatcacg cgcgaagaac ttgatgccat
     3061 tgtcccagta gggattgtgc gatgcgctga tgacgatgcc ggcggcagcg cgcagcgtgc
     3121 gcgtcagata ggcgatggcc ggcgtcggca tcggcccgag cagcgcgata ttgacgccgg
     3181 cggctgaaag cccggcttcc agcgccgatt cgaacatgta acccgaaatc cgcgtgtcct
     3241 tgccgatcag gactttctgg gcaccggcgc cagccagcac cttgccggcc gcccacccca
     3301 gcttgagcat gaaatcggcc gtgaccggtg cctcgccgac ccgcccccga atgccgtcgg
     3361 taccgaaata acgcatctgt ctacccttat tcagtctggc cacgaccggc cggacggctg
     3421 gtctggcgct tccaaaaatt ccagaaaccc aaaaaccgga atgaccggga cgctgcgctc
     3481 agggtgcagc gcgcacggcc ctcaggatgc ccagcgcctc gaccgtctcg cgcacgtcgt
     3541 gcacccgcac gatgcgggca ccggcggcgg ccgcgagcag tgctgcggcc acgctgcccg
     3601 tgacccgccg ctccagcggc aggtcgagca cggcgccgat catggacttg cgcgacagcc
     3661 ccacgagcac cggccattgt ccaccggtca gcgcatcgag gtggcgcagc aatgaaaggt
     3721 tgtgtgtcag cgtcttgccg aagccgaagc cgggatcgag cacgatgcgc tgcgacccga
     3781 tgccggccgc caggcaggca gcggcgcggg ccaacaaaaa ctccctgacc tcggccacga
     3841 catccgcgta cgccggcgcc tgctgcatcg tgcgcggctc tccctgcatg tgcatcagac
     3901 agaccgcggc ctgactgtcg gcaacgacct ccagcgcacc acaggcacgc agggcccgga
     3961 cgtcgttgat gaggccggca ccggccgcga tggcggcacg catgaccgcc ggcttgctgg
     4021 tgtcgaccga aaccggcacg gccagcccgg cactcaggtg ctcgaccagc ggaatgaccc
     4081 ggcgcagctc ctcggcctcg gatacctcgg cggcaccggg ccgggttgat tcaccgccga
     4141 tgtcgatcag cgccgcaccg gcctcgacca tggcgtacgc atgttcgagc gccgctgccg
     4201 gctcgatcca gcgaccgcca tccgaaaagg agtcgggggt cacgttcagg acccccatga
     4261 cgacgggccc tgaaagatcc agggcccgtg aaccgcattg caactgcacg ataccggtca
     4321 gcctcagtgc tgctgcgcgg gaccaccgat cgggccggca tcgcccttgc cgccattcga
     4381 tgccgaggcc cccggcccgg cggccggcgg ttcgacatcg gcgtaatcct gcggcggtcg
     4441 cggttcccga ccggcgagga tgtcgtcgat ctggtcggaa tcgatcgtct cgtacttgat
     4501 cagcgcctcg gacatcttgt gcagcgcgtt catgtgatcg cgcaggatct gctcggcacg
     4561 ctggtaattg cgatcgatga tcgaacggat ttcctcatcg atcacgtgcg cggtctcgtc
     4621 cgagacgttc ttgtggcgcg tgaccgaacg gccgaggaag acctcgccat cgtcctcgct
     4681 gtaggtcagc ggcccgagcc ggtccgacag gccccacttc gtgaccatgt tgcgggcgat
     4741 ctcggtcgcc cgctcgatgt cgttcgaggc accggtggtc accgaatccg agccgaagat
     4801 cagcacctcg gcgatgcgcc cgccgaacag gctggagatc tggctctcca gacgctggcg
     4861 cgagtagctg tagcggtcct gttcgggcag gaacatcgtc acgccgaggg cgcggccacg
     4921 cgggatgatg ctgaccttgt agaccggatc gtggtccgga acggtgcgcc cgacgatcgc
     4981 atgaccggcc tcgtggtagg cggtcagctt cttctcggac tcgctcatga ccatggactt
     5041 gcgctcggcg cccatcatga tcttgtcctt ggccttctcg aactcctcca tgccgacttc
     5101 gcgcttgttg gcacgggccg cgaacagcgc ggcctcgttg accagattgg cgagatcggc
     5161 accggagaag cccggcgtgc cgcgggcgat caggctcgga cgcacgtcgc cgccgaccgg
     5221 caccttgcgc atgtgcacct tcaggatctg ctcgcgaccg cgcacgtccg gcagcggcac
     5281 gaccacctgc cggtcgaagc ggccggggcg cagcagcgcc ggatcgagca cgtccgggcg
     5341 gttggtcgcg gcgatgacga tgatgccctc gttgccctcg aagccgtcca tctcgaccag
     5401 cagctggttc agcgtctgct cgcgctcgtc atgcccgccg ccgagaccgg cgccgcggtg
     5461 acggccgacc gcgtcgatct cgtcgatgaa gatgatgcac ggcgcgtgct tcttggcctg
     5521 ctcgaacatg tcgcgcacgc gcgaggcacc gacgccgacg aacatctcga cgaagtcgga
     5581 accggagatc gagaagaacg gcaccttggc ctcgccggcg atcgccttgg cgagcagcgt
     5641 cttgccggtg ccgggcgagc cgaccatcag cacgccgcgc gggatcttgc cgccgaggcg
     5701 ctggaacttg cccggatcgc gcaggaactc gacgagctcg ccgacttcct ccttggcctc
     5761 gtcgcagccg gcgacatcgg cgaaggtgac cttgatctgg tcctcgctca tcatgcgcgc
     5821 ccgcgacttg ccgaagctca tcgcaccgcg tccgccggcc ccgccctgca tctggcgcag
     5881 gaagaacacc cagacgccga tcagcagcag catcgggaac cagctgatga agatctgcat
     5941 ccagaccgac tgctgctccg gcggccggcc ttcgacatcg acgcggttgt cgagcagcgt
     6001 gccgatcatc gccttgttgt cggtctcggg gctgtaggtc atgaacttct cgccggtgct
     6061 ggtgcgaccg gagatcgagc gcccgtcgat cgacacgtgc gcgacgcgcc cctgcttgac
     6121 ctcttcgagg aactgcgaat acgtcagctg ctgagacggc gacgtgcgcg gtccgaagct
     6181 gttgaacaca gacatcagga cgacggcaat caccacccag agcaggatgt ttttgaccat
     6241 gtcgttcaag gtggcacctc gccgtgcgaa agaccggaca ccgaccccgg cgcccggatg
     6301 aagaatgacg ttactacgac cggaaattcc gggccagcag atacagttcg gcgctgcgcg
     6361 cccgcgaggc cttcggcttg cgcgtggcca ccgagccgaa atccgcacgc agatccttca
     6421 gcagcgcatc gaagccctcg ccctggaaca ccttgaccag gaagtcacca ccgggcctga
     6481 gcgtctgacg ggcgaagtcg agcgcgagtt cgaccagata catctgcttg ggctggtcga
     6541 cggccttcag accgctggta ttgggggcca tgtcggaaat cacaaggtcc accggcgtgc
     6601 cggccagcgt cgcgagcagc tgcgcgaaca cggtctcctc gcggaaatcc ccctggatga
     6661 agtcgacgcc ggccagcgca tccatcggca ggatgtcgag cgcgagcacc cggccgcgct
     6721 cgccgagcgc gcgcgccgcg tactgcgacc agccccccgg cgcggcgccg agatcgacga
     6781 gcgtcgtgcc gggcttcagc agccggtcgc gctcgttgat ttcctgcagc ttgtagacgg
     6841 cgcgcgagcg ccagccctcg gcctgggcgc gccggacgaa ctcatcgtcg aagtgctcct
     6901 tcagccagcg gtggctgctg cggctacgcg ccatcgccgg cactcctgcg agcctgctcg
     6961 ccgagcacgc gccgcgcgcg cagcaccagc cacagcgcgc cggcgacgcc gtaagcggcc
     7021 agcgccagca gctgccacag cggctcggca aacacgcgcc cgaccaccgg caccagcagt
     7081 agtccgacca gcaggatcac gcccagctcg aacttgacgc cgttcagcgg gctcgcggca
     7141 accgacaggg agccgcgtgc tacactccgc gccccttttt cctcagacat ttccactccg
     7201 gtcagacatg ctcaagcaaa gccagaagcg tcacctgaag gccctcgccc acccgctgaa
     7261 acccgtggtg atcgtcggcg ccaacggcgt gacgcctgcc gtcctcgacg aagtccgcct
     7321 cgcgctggag catcacgagc tgatcaaggt gcgcgtcaac gccggcgacc gcgaacagcg
     7381 cgaggcactg atcgccgaca tcgtcgaggc caccggctgc gaactggtcc agcgcatcgg
     7441 ccacatcgcg gccttcttca gacgcaatcc ggacaagccg cgcatcacgc tgcccgcctg
     7501 agcgtctcgc gcacgcgcag gaccagccag cccagaccga gcaggctctc ggcgagatac
     7561 agcagcgccg cgatgccgtg caggcgcgca aacatcgccc ccccgctctc cccgaccgcc
     7621 ttcagctccg ccatgcgcgg ctgcaggccg aattcgccgg tcagcacgag cagcagcatc
     7681 agcgccggca gcagcaccag cgtcagcgat gcctgcgccc gccgccagcg ccacagtgcg
     7741 agggcgagca gtgccgcacc gcaaccgagc cccagccagg ccacgagcgt gaacagccgg
     7801 ccggcaacct gacctgccag cgcgcgctcc ggcagcgtcg cgaacaggac cggcgcgacc
     7861 agatagccga ccgtccacag cgaaccgagc cagacgacgg tcagcaactg ctccagcgca
     7921 accggccccc agtcacgcag ccgcgcccgg acagggctgc tcatgggcat cgccccgcgg
     7981 aacacacgat cgcgaccttg ggacaggcaa acggccggtg accgtcaacg gcatcggcac
     8041 gacagaggcg ggcggcaggc agggggcgga aggaagacat cgcgggcgat tgtgctttgc
     8101 gggctcgccg ccggcaacgc cgatgcgcag ccaagcggta tgcacggatg acgggcagga
     8161 cggccggctg cctgcgccgc acccgccatg cctgctgccc ggttacggca cgacggcgct
     8221 gccgtatccg gcgccggggg ttgcacccgg gttttgatac ccccggaaca ctggcgggtg
     8281 acgggtctcg tccgccggtt ctgcgatcgt tgcagccgga ttccacaatt tacaaacagc
     8341 aagtattctc attaacatcc gcagtcattc ctgttcatga cgcccggatg aagccgtgat
     8401 cgcccgccct gcgccactgc gccccgctcc cgccgccctg ccggccgccc tgctgtcgtc
     8461 gcgaccaccg gcgctgcgcc gcgggctgcg caccggcggc accttcgctg cggccctggc
     8521 cctgcacgtc ggcgcactcg gtcactggac gacgcctgaa accctgccgc agccgctgcc
     8581 gatggcgccg ctggtcgtcg acctgatcga agccgtaccg cccccggacc cgctcgccgc
     8641 tgccccggcc gagacggctc ccccacccga acccgaagcg ccgccacccg aaccgccgcc
     8701 cccgcccgaa ccgctgccgc aggcgatcac tgcgccgccg aagcctcccc tgcccgcacc
     8761 gccgaagcca aagccgaagc cggtcgtgca gccgccgaaa ccgaagccga ccccccgtcc
     8821 gccggccccg aatgcggcgc gcagcgaaac cccgccctcg ccgctgccac cggcgccggt
     8881 cgccgccgcg ccctcgacga cggctgcgaa cttcagcgcc gcctacctgc gcaatccgcc
     8941 gccgcgctac ccgcccgact cacgcagtgc cggcgaggcc gggcgcgtgc tgctgcgcgt
     9001 gcgcgtcacg gccgagggcc gcgccgagtc ggtcgagatc gccgcgagca gcggccatgc
     9061 gcgccttgat gacgcggcgc tcgcggccgt gcgccgctgg cgtttcgtgc cggcgcagca
     9121 ggccggcgcg ccggtcgccg ccaccgtcac cgttccgatc ctgttcaagc tggagtctta
     9181 agcccgatgt ttccgaccga cgcgctgttt ccgcccggct tcctcgccga ggtcctcgcc
     9241 gatctgcctt cgggtctggc ccacctgttc gccggtgccg acgccgtcag cctcggctcg
     9301 gcgctggcgc tgctggcgat gtcgctgctg tcgtggacgc tgatcctcgc caagctcgcc
     9361 gcacggctgg cgcgcatgcc ggcgcgccgg cgcgcgcagc gcgtctgggc cgagaccgaa
     9421 acggtcgccg aggccgagca gcgcctgcag cgcacgcccg gtgccgaagc ctggctggag
     9481 ctgttcgccg atggccgtgc cgcggccgac tggctgcgcc gccacggccc cggccacgcc
     9541 ggcgacccga tcgccgcggc gctgacgcag acgctgaacc ggctcggcgc gcgcttcgag
     9601 agcgggctga cggcgctcgc gtcgatcggc agcacggcgc cgttcgtcgg tctgctcggc
     9661 acggtctggg gcatccagca cgcgctggtc gccatcgccg ccgccggcag tgcgcagatc
     9721 gacaaggtcg ccggtccggt cggcgaggcg ctgatcatga ccgccttcgg tctggcggtc
     9781 gcgctgccgg cggtgctggc ctacaacgcc tgcacgcgcg gccagcgcct gctcagtgcc
     9841 gaactcgaag gcttcgcgca tgcgctgcac gcgcgcctgc acgccgaact gaacggtgtg
     9901 ccggattccg gcgccgcgct gctgccggcc gcggccgtgc caccggcccc cgtcagcgaa
     9961 gccgtcccgc gtcccgcagc ggcgagcgag cgcccggccg aatcaccgca ggccggcatc
    10021 gtgcgcctct gcccggccgc gggggtgtga catggccttc ggcagcctcg gcagcagcca
    10081 ttcccgcccg aacgccgaga tcaacaccac gccgctggtc gacgtgatgc tcgtgctgct
    10141 ggtgatcttc atcctgaccg cgccgctgtt cacgcacacg gtcaagctcg acctgccgcg
    10201 cgccgcgcac cgcaccgatc cgcagccgcc gccggcggtg acgctgtcgc tcgatgccgc
    10261 cggcgcgctg ttctgggaca acgccgcgct cgccgaaagc gaactcgcgc cccggctcgc
    10321 cgccctcgcc gcgcagcagc cgcagcccga actgcacctg cgcgccgatc gcgagacccg
    10381 ctacgaacgc atcgcgcagc tgatggccgc ggcgcgcaat gccggagtca accgcatcgg
    10441 cttcgtgacc gatccgcagc cggccgcgac gcactgaggg cggatcggcg ggcaccggcg
    10501 gcgctcctct ttccgccggc tccggacaag ccgtcggcag ctcgcgcggg cctgaaagac
    10561 ggcacgg
//
LOCUS       DAOQIE010000116        10543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3156_length_10543_cov_6.982933, whole genome shotgun sequence.
ACCESSION   DAOQIE010000116 DAOQIE010000000
VERSION     DAOQIE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_10543_cov_6.982933"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK144_08850"
     CDS             complement(<1..763)
                     /locus_tag="PK144_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019594967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF65873.1"
                     /translation="MSPVTLLRDAPLRTLLGACLPGGGRARLSILIYHRVLATHDPLQ
                     PDIPDAALFRRHMHLLARQFRPLPLLEAVRRLREGSLPARAVCVTFDDGYADNAAIAL
                     PLLQQSGVPATFFVSTGFLEGGRMFNDTVFEVVRRLPEGTLSPDEVPGLDAPLAIGAG
                     AASRIAAAHALIGAVKYLEPAARQAVVGRLAARVRDLPDDLMMTHAQLRALHAAGMEI
                     GGHTVSHPILTRTPAAVARAEIAGGRAGLEALIDAP"
     gene            complement(760..2088)
                     /locus_tag="PK144_08855"
     CDS             complement(760..2088)
                     /locus_tag="PK144_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HNF65874.1"
                     /translation="MRDIAILLIVTYGLIRTVREPVIGILLWCWIGYMNPHRLSYGFA
                     YSMPWAFMTAAVLIPSLLFSKEPKKLPGHWLVSVWLAFVVWMCITTVFAQDFSTSAEQ
                     LDKVLKIQLVNAFMMMIMGTRERLNMLLWVIVISIGFFGLKGGIFTILGGGGSKVWGP
                     PGSFIADNNEIGLAMLTTMPLMYYLRQQSASKWVRHALALTMLMTAFAVIGTSSRGSM
                     IAGLTAAAFLWLRSPHKIGLGLGLLVLLPVLYLFAPSSWHERMGTIETYDQDASAMGR
                     INAWGMAFRLASNRLTGGGFVPWTPENFAKYADVPSDVHAAHSIYFGVLGDHGWPGLI
                     LFVAIAVGSLVSVSRALKICRAAPELTWLSQMLAMVQVSLIVFLVGGSFYSLAYFDLY
                     WNLVTIVMLGHVMARRYEAEHPELFVRKTLTGRPAPPTREERLARPGNPR"
     gene            complement(2095..2934)
                     /locus_tag="PK144_08860"
     CDS             complement(2095..2934)
                     /locus_tag="PK144_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNF65875.1"
                     /translation="MSLDLRRFAKLPLYLRAFGPLAGLRLYAAIERAGLPVKSETVQA
                     HAVPGYDAPVSLRRARADHATFWQCLVQRQYDLDRFPQTARLQQTYEAMLARGERPLI
                     IDGGANIGLAALWLARRFPQAQIVSVEPDTGNLALLRRNLAGLGARAQVLDGGLWPRS
                     GRLRIVNPESGSAAFRVEETTGDGPGTLRAYTIDEICTLAGCREPLIVKLDIEGAQQY
                     LFASATEWVGRAHLITLELDDWLFPWRGSSRPFFRVLGEHPFDYLLGGESVFCFRDAG
                     AAV"
     gene            complement(2982..3890)
                     /locus_tag="PK144_08865"
     CDS             complement(2982..3890)
                     /locus_tag="PK144_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65876.1"
                     /translation="MKLLQQRFAQLRRKWHELQFRRAAAGVRSIGPIRLAAEGPIVLS
                     MVQQRDVDAYLLALGTFVRRLPARRIVVVADPSLDAADQAVLRRQVPDIEIVAQHDFR
                     PEGLPSGGCWERLVAITRYVSEGYVIQLDADTVATGPLDEVAACVRDNRAFTLGTDDH
                     FVLQPASEAADFARSRASPDAHIQVHAEAALDRLPAEVFPAYIRGCAGFAGFPAGYEM
                     LPKLLQLSAVMEQAIGARWRQWGSEQFASNVMVANAPASCVLPHPKYCAPHRRRADSV
                     FLHFIGYVRYIDGTYARLARGYLKTA"
     gene            complement(3891..5405)
                     /locus_tag="PK144_08870"
     CDS             complement(3891..5405)
                     /locus_tag="PK144_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF65877.1"
                     /translation="MNITQRMQIGASLMLLAKFLERGLGLISTVILARLLLPGDFGLV
                     AMAAPIVMFIELLRSFSFDMALIQKADAEAAHFDTAWTLNLLCTTLIALLMVLAAAPA
                     ADFYQEPRFAPVMAWLALGTFIQGFENIGTVNFRKELDFGQEFRLMLYKKLASITLTA
                     LLAWWLRNYWALVWGTLASRVFGVLLSYHMSHYRPSLRLSHWRDLMHFSKWMVVNNLL
                     FFLNLRGVDFVIARLFGAAVLGQYLVAIEISTILSVELVAAANRAFFPGFVKLSGEPN
                     TVVRHCTTVMAAAAMWLLPLAMLLYLLGDLFVHAMFGDKWPLTGELIGEFGLFGALTA
                     FTGSLSYAYMAVGHPRFSSVAAGVQSLIVLGLLVLNGYWGRPQWIGHCFVLTACLVLF
                     VHAAMVKHVFPALQLLRWLRGVARMSLAAAGAGLVISSLKAPLLGSGLPEPVVLVGLG
                     IGGVALYGAGVWLLWQLDHCKDGPERSILTRIPRLQAYIERRRSPYDWIAEGRI"
     gene            complement(5402..6625)
                     /locus_tag="PK144_08875"
     CDS             complement(5402..6625)
                     /locus_tag="PK144_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase, exosortase A
                     system-associated"
                     /protein_id="HNF65878.1"
                     /translation="MRILHVLDHSIPLHSGYTFRTRAILREQRRLGWSTGHVTGSKQV
                     TPASPYAGPEENVDGLHFYRTPPDLRRRAGWPLFDQWAVVTGLSARLDEVIEDFRPDV
                     LHAHSPVLNGLAALSAARRHGLPLVYEVRAFWEDAAVDHGTTREGSLRYRATRALETF
                     VLERADAVTTICHGLRRDILGRGVPADRVTVIPNAVDVGQFGPGGQPDPALLARHGLA
                     GKRVLGFIGSFYAYEGLDLLLEALPQIRAQAPDVQLLLVGGGPQDGALRAQAERLGLA
                     DAVHFTGRVPHAEVNAWYDLIDVLCYPRHPMRLTELVTPLKPLEAMAQGRLFVASDVG
                     GHRELIRDGETGALFRAGSAEALAERVLRLLAAPASWPKLRAAGREFVETERNWAVSV
                     GRYRGVYARALGTGA"
     gene            complement(6631..8559)
                     /locus_tag="PK144_08880"
     CDS             complement(6631..8559)
                     /locus_tag="PK144_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018951560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HNF65879.1"
                     /translation="MCGIAGIVDLRERRNIEADVLASMNQVQFHRGPDEGGVHIEPGV
                     GLAHRRLSIIDLSSGQQPLWNEDHTVVVVFNGEIYNYQGIAAELQALGHTFRTHSDTE
                     VIVHAWEQWGEACVTRFRGMFAFALWDRNTETLFLARDRLGKKPLHYALLPDGQLIFG
                     SELKILAAHPRFPRALDPLAVEDYFAYGYIPDPRSIYRAALKLPPAHTLCLRRGEPLP
                     APKSYWDAPFAPLPAMSVEDAGAELIERLREATRIRLMSEVPLGAFLSGGVDSSAVVA
                     MMAGLSDEPVKTCSIAFDDPRFNEAQFAQQVADRFRTEHRVETVDPDDFDLIDRLAWL
                     YDEPYADSSALPTYRVCQLARRSVTVALSGDGGDENLAGYRRYKWHSYEERLRSALPL
                     GVRRPLFGLLGKVYPKADWAPRVLRAKTTFEAMARDTVEGYFHGVSVMSDRMRAPLYS
                     AAFRRELQGYHALEVMRAHAANAPTEDPLSLIQYIDLKTYLPGDILTKVDRASMAHSL
                     EVRVPLLDHELVEWISGLPPDFKLRGGEGKYVFKKALEPVLPHDILYRPKMGFAVPLA
                     AWFRGPLAARLREAVLGATMRACGVFDMDYLATLVGQHQSGRRDHSAALWSLLMFESF
                     LRNAGVGADAGPVWREAA"
     gene            8848..10398
                     /locus_tag="PK144_08885"
     CDS             8848..10398
                     /locus_tag="PK144_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF65880.1"
                     /translation="MDALWICLAFAAGLLAHRIGLPPLVGYLAAGFGLHAAGERSGPA
                     IEQVAHLGVLLLMFTIGLKLRLQYLARPEIWGGGLAHLGLFALLAGVLVWLLAAQAPG
                     PATALGLALGFSSTVIAAKSLEAKQELRSFHGRVAIGILIVQDLAAVGLLSVAAEAPH
                     PGAAALLLLPLARPLLLKLLSACGHDELLLVFGLALAAGAGALFHALGLSAELGALAA
                     GALLAAHGRAGELASALWGLKEAFLLGFFLQIGLAGGPDAATFFGALGLLLLLPLKAA
                     ALFLVLIAFRLRARSAFLSALSLASYSEFALIVAQLGVQAGWLDAQWLTLLALAIAAS
                     FALGAPLNRLAHPLFERWEARLLGFERHVRHPDEEPIHLGAAHLLVIGMGRAGTVAYD
                     YLVQHERVVGLDSDLSRVGDHLHAGRRVLYADAEDPGLWHHLHLSGIRAVLLMMRDAE
                     ARVIAARQLRRHGFTGLVAAISHYPDEAAAVTAAGADMTFTRLDQVGLGLAEHVLGEL
                     KQRAAAGG"
BASE COUNT         1642 a         3727 c         3686 g         1488 t
ORIGIN      
        1 ccggagcatc gatgagagct tcgagtccgg cacggccccc ggcaatctcg gcgcgcgcca
       61 ccgcggccgg cgtgcgcgtc aggatcggat ggctgaccgt gtggccgccg atctccatgc
      121 cggcagcgtg cagcgcgcgc agctgcgcgt gcgtcatcat caggtcgtcg ggcaggtcgc
      181 gcacgcgcgc ggccagccgc ccgaccaccg cctgacgcgc ggccggctcc agatacttga
      241 cggcgccgat cagtgcgtgc gcagccgcga tccgggacgc cgcaccggcg ccgatcgcga
      301 gcggcgcgtc gagccccggc acctcatcgg gcgacagcgt gccttccggc agccggcgca
      361 cgacctcgaa caccgtgtcg ttgaacatgc gcccgccttc gaggaagccg gtcgacacga
      421 agaaggtcgc cggcacgccg ctctgctgca ggagcggcag cgcgatcgcg gcgttgtcgg
      481 cgtagccgtc gtcgaaggtc acgcacacgg cccgcgccgg caggctgccc tcgcgcaggc
      541 ggcgcacggc ttcgagcagc ggcagcggcc ggaactggcg ggccagcagg tgcatgtgcc
      601 ggcgaaacag cgcggcatcg gggatgtcgg gctgcagcgg gtcatgggtg gcgagcacgc
      661 ggtgatagat caggatgctc agccgcgccc gcccgccgcc gggcaggcag gcaccgagca
      721 gcgtgcgcag cggcgcgtcc cgcagcagcg tgaccggact catcgcggat tccccggcct
      781 cgccagccgc tcctcgcgcg tcgggggcgc cggccggccg gtcagggtct tgcgcacgaa
      841 cagctccgga tgttcagcct cgtagcgccg cgccatgacg tgaccgagca tgacgatggt
      901 caccagattc cagtacaggt cgaagtaggc gaggctgtag aagctgccgc cgaccaggaa
      961 cacgatcagg ctgacctgca ccatcgccag catctgcgag agccaggtca gctccggggc
     1021 ggcgcggcag atcttcagcg ccctcgacac gctgaccagc gagccgactg caatggccac
     1081 gaacaggatc agacccggcc agccgtggtc gccgagcaca ccgaagtaga tgctgtgggc
     1141 cgcatgcacg tcactcggca catccgcgta ttttgcgaag ttctccggcg tccacggcac
     1201 gaagccgcca ccggtcaggc ggttgctggc cagccggaag gccatgcccc aggcgttgat
     1261 gcgccccatc gccgaggcgt cctggtcata ggtctcgatc gtgcccatgc gctcgtgcca
     1321 cgaagacggt gcgaacaggt acagcaccgg cagcagcacg agcaggccga ggccgagccc
     1381 gatcttgtgc gggctgcgca gccacaggaa ggctgcggcg gtcaggccgg cgatcatgct
     1441 gccgcgcgaa ctggtgccga tcaccgcgaa cgccgtcatc agcatggtca gcgccagcgc
     1501 gtggcggacc cacttgctgg cgctctgctg gcgcaggtaa tacatcagcg gcatcgtcgt
     1561 cagcatcgcc agaccgatct cgttgttgtc ggcgatgaac gagcccggcg gcccccagac
     1621 cttggagccg ccgccgccga ggatcgtgaa gatgccgccc ttgaggccga agaagccgat
     1681 cgagatcacg atcacccaca gcagcatgtt cagccgctcg cgcgtgccca tgatcatcat
     1741 catgaaggcg ttgacgagct ggatcttcag caccttgtcg agctgctcgg cggaggtgct
     1801 gaagtcctgc gcgaacaccg tcgtgatgca catccagacc acgaacgcca gccacaccga
     1861 caccagccag tgcccgggca gcttcttcgg ctccttcgag aacagcaggc tcgggatcag
     1921 caccgccgcg gtcatgaacg cccacggcat cgagtacgcg aagccgtagc tcaggcggtg
     1981 cggattcatg tagccgatcc agcaccacag caggatgccg atcaccggct cgcgcaccgt
     2041 gcggatcagg ccgtaggtga cgatcagcag gatggcgatg tcacgcatcg gcgttcacac
     2101 cgcggccccg gcatcgcgga agcagaacac gctctcgccg ccgagcaggt agtcgaacgg
     2161 atgctcgccg agcacccgga agaacggccg gctgctgccg cgccacggga acagccagtc
     2221 gtcgagttcg agcgtgatca ggtgcgcgcg cccgacccat tcggtcgcac tcgcgaacag
     2281 gtattgctgc gccccctcga tgtcgagctt gacgatcagc ggctcgcggc agccggcgag
     2341 cgtgcagatc tcgtcgatcg tgtacgcgcg cagcgtgccg gggccgtcgc cggtggtttc
     2401 ctcgacgcgg aaggccgcgg agccggactc ggggttgacg atgcgcagcc gcccgctgcg
     2461 cggccagaga ccgccgtcga gcacctgcgc gcgcgcgcca agcccggcga ggttgcgtct
     2521 gagcagcgcc agattgccgg tgtccggttc gaccgagacg atctgcgcct gcgggaaacg
     2581 ccgcgccagc cacagcgccg ccagcccgat gttggcgccg ccgtcgatga tcagcggccg
     2641 ctcgccgcgc gcgagcatgg cctcgtaggt ctgctgcagg cgcgccgtct gcgggaagcg
     2701 gtcgaggtcg tactggcgct gcaccaggca ttgccagaag gtcgcgtgat cggcgcgggc
     2761 ccggcgcagg ctcaccggcg cgtcgtagcc cggcaccgca tgcgcctgca cggtttccga
     2821 cttcaccggc agcccggcgc gttcgatggc ggcatacagg cgcagccccg ccagcggccc
     2881 gaaggcccgc aggtacaggg gcagtttcgc gaagcgccga agatccagac tcatgccaga
     2941 aactctcccc agaaactctc cgtgatgccg ccgtgcgggc ctcaggcggt cttcaggtag
     3001 ccgcgtgcga gccgcgcgta ggtgccgtcg atgtagcgca cgtagccgat gaagtgcagg
     3061 aacaccgaat cggcccggcg ccggtgcggc gcgcagtact tcgggtgcgg cagcacgcag
     3121 ctcgccggcg cgttggcgac catcacgttc gacgcgaact gctccgagcc ccactgccgc
     3181 cagcgcgccc cgatcgcctg ctccatgacc gccgacagct gcagcagctt cggcagcatc
     3241 tcgtagccgg ccgggaaacc ggcgaagccg gcgcagccgc ggatgtaggc cgggaacacc
     3301 tccgccggca gccggtcgag cgcagcctcg gcgtgcacct ggatgtgggc gtccgggctg
     3361 gcacgcgagc gtgcgaagtc cgccgcctcg ctcgccggct gcagcacgaa gtggtcatcg
     3421 gtaccgagcg tgaacgcgcg gttgtcgcgc acgcaggcgg ccacctcgtc gagcgggccg
     3481 gtcgcgaccg tgtcggcatc gagctggatc acgtagccct cgctcacgta gcgcgtgatc
     3541 gcgaccagcc gctcccagca gccgccgctc ggcaggccct cggggcggaa atcgtgctgc
     3601 gcaacgatct cgatgtccgg cacctgccgg cgcagcacgg cctgatcggc cgcatcgagg
     3661 ctcggatcgg cgacgacgac gatgcgccgc gccggcagcc ggcgcacgaa ggtgccgagg
     3721 gcgagcaggt aggcatcgac atcgcgctgc tggaccatcg acagcacgat cggcccctcg
     3781 gcggcgagcc ggatcggacc gatgctgcgc acgccggccg cggcgcgccg gaactgcagt
     3841 tcatgccact tgcgccggag ctgcgcgaat cgttgctgca ggagcttcat tcagatgcga
     3901 ccttccgcga tccagtcata agggctgcgc cggcgttcga tgtacgcctg caggcgggga
     3961 atccgggtca ggatgctgcg ctcggggccg tccttgcagt gatcgagctg ccagagcagc
     4021 cagacgccgg cgccgtagag cgccacgccg ccgatgccga gcccgaccag cacgaccggc
     4081 tccggcaggc cgctgccgag cagcggcgcc ttcaggctgc tgatgaccag accggcgccg
     4141 gcggcggcga ggctcatgcg cgcgacgccg cgcagccagc gcagcagctg cagggccggg
     4201 aacacgtgct tgaccatcgc cgcatgcacg aacagcacga ggcaggcggt cagcacgaag
     4261 cagtgcccga tccactgcgg ccgcccccag tagccgttca gcaccagcag ccccagcacg
     4321 atcaggctct gcacgccggc ggccaccgaa ctgaagcgcg ggtggccgac ggccatgtac
     4381 gcatacgaca gcgagccggt gaacgccgtc agcgcaccga acagcccgaa ctcgccgatc
     4441 agctcgccgg tcagcggcca cttgtcgccg aacatcgcat gcacgaacag gtcgccgagc
     4501 aggtacagca gcatcgccag cggcagcagc cacatcgccg ccgccgccat caccgtcgtg
     4561 cagtggcgca cgacggtgtt cggctcgccg gagagcttga cgaaacccgg aaagaaggca
     4621 cggttggcgg cggccaccag ctcgaccgac aggatcgtgc tgatctcgat cgccaccagg
     4681 tactggccga gcaccgcggc accgaacagg cgggcgatga cgaaatcgac gccgcgcagg
     4741 ttcaggaaga acagcaggtt gttgacgacc atccacttcg agaagtgcat caggtcgcgc
     4801 cagtggctca ggcgcagcga cggccggtag tggctcatgt ggtaggacag cagcacgccg
     4861 aacacccggc tcgccagcgt gccccagacc agcgcccagt aattgcgcag ccaccacgcc
     4921 agcagcgccg tcagcgtgat gctggcgagc ttcttgtaca gcatcagccg gaattcctgg
     4981 ccgaagtcga gttccttgcg gaagttgacg gtgccgatgt tctcgaagcc ctggatgaag
     5041 gtgccgagcg cgagccaggc catcaccggc gcaaagcgcg gctcctgata gaagtccgcc
     5101 gccggcgcgg cggcaagcac catcagcagc gcgatcagcg tcgtgcagag caggttcagc
     5161 gtccaggcgg tatcgaaatg tgcggcttcg gcatcggcct tctggatcag cgccatgtcg
     5221 aagctgaacg agcgcagcag ctcgatgaac atcacgatcg gcgcagccat cgccaccagg
     5281 ccgaagtcgc ccggcagcag cagccgcgcg aggatcacgg tgctgatcag cccgagcccg
     5341 cgttcgagga acttcgcgag cagcatcagc gaggcgccga tctgcatgcg ctgcgtgatg
     5401 ttcatgcacc ggtcccgagt gcgcgcgcgt agacgccgcg gtagcgcccg acgctgaccg
     5461 cccagttgcg ttcggtctcg acgaactcgc gcccggccgc gcgcagcttc ggccacgagg
     5521 ccggggccgc gagcagcctc agcacgcgct cggccagcgc ttcggcgctg ccggcgcgga
     5581 acagcgcacc ggtctcgccg tcgcggatca gctcgcggtg gccgccgaca tcggaggcga
     5641 cgaacagccg gccctgggcc atggcttcga gcggtttgag cggcgtgacc agctcggtca
     5701 gccgcatcgg atgacgcggg tagcagagca cgtcgatcag gtcgtaccag gcgttgacct
     5761 cggcgtgcgg cacgcggccg gtgaagtgca cggcatcggc gagccccagg cgctcggcct
     5821 gcgcgcgcaa ggcgccatcc tgcggcccgc cgccgaccag cagcagctgc acgtcggggg
     5881 cttgcgcgcg gatctgcggc agcgcttcga gcagcaggtc cagcccctcg taggcgtaga
     5941 acgaaccgat gaagccgagc acgcgcttgc cggcgaggcc gtggcgcgcg agcagcgccg
     6001 gatcgggctg gccgccgggg ccgaactgcc cgacatcgac ggcgttcgga atcaccgtca
     6061 cccgatcggc cggcacgccg cgtccgagga tgtcgcggcg caggccatga cagatcgtgg
     6121 tcacggcgtc ggcgcgttcg agcacgaagg tttcgagcgc gcgcgtcgcg cggtagcgca
     6181 ggctgccttc gcgggtcgtg ccgtgatcga cggcggcgtc ctcccagaac gcgcgcacct
     6241 cgtagaccag cggcaggccg tggcggcgcg ccgccgacag cgccgcgagg ccgttcagca
     6301 ccggcgaatg cgcgtgcagg acatccggac ggaagtcctc gatgacctca tcgagccgcg
     6361 ccgacagccc ggtgaccaca gcccactggt cgaacagcgg ccagccggcg cgccggcgca
     6421 ggtccggcgg cgtgcggtag aagtgcaggc cgtcgacatt ttcctcgggg ccggcgtacg
     6481 gcgaggccgg cgtgacctgc ttgctgccgg tcacatgccc ggtgctccag ccgagccggc
     6541 gctgctcgcg caggatcgcg cgcgtgcgga aggtgtagcc gctgtgcagc gggatcgaat
     6601 gatcgaggac gtgcaggatg cgcatgcggc tcaggcggcc tcgcgccaga ccggaccggc
     6661 gtcagcgccg accccggcgt tgcgcaggaa ggactcgaac atcagcagcg accagagcgc
     6721 cgccgagtgg tcgcgccggc cgctctggtg ctgcccgacc agcgtcgcga ggtagtccat
     6781 gtcgaacacg ccgcaggcgc gcatggtcgc gccgagcacc gcttcgcgca ggcgcgcggc
     6841 cagcggtccg cggaaccacg ccgccagcgg caccgcgaag cccatcttcg gccggtacag
     6901 gatgtcgtgc ggcaggaccg gttcgagcgc cttcttgaac acgtacttgc cttcgccgcc
     6961 gcgcagcttg aagtccggcg gcaggccgct gatccactcg accagctcgt gatcgagcag
     7021 cggcacgcgc acctccagcg aatgcgccat gctggcgcgg tcgaccttgg tcaggatgtc
     7081 gccgggcagg taggtcttga ggtcgatgta ctggatcagc gacagcggat cctcggtcgg
     7141 ggcgtttgcg gcgtgcgcgc gcatgacctc gagcgcgtgg tagccctgca gttcgcgccg
     7201 gaacgccgcc gagtacagcg gtgcgcgcat gcggtcgctc atgaccgaga cgccgtggaa
     7261 gtagccctcg accgtgtcac gggccatggc ctcgaaggtg gtcttggcgc gcagcacgcg
     7321 cggcgcccag tcggccttcg ggtagacctt gccgagcagc ccgaacagcg gccggcgcac
     7381 gccgagcggc agtgccgagc gcaggcgctc ctcgtagctg tgccacttgt agcggcggta
     7441 gccggcgaga ttctcgtcgc cgccgtcgcc ggagagcgcg acggtgacgc tgcggcgtgc
     7501 gagctggcag acgcggtagg tcggcagcgc cgaggagtcg gcgtagggct cgtcatacag
     7561 ccaggcgagc cggtcgatca ggtcgaagtc gtcgggatcg acggtctcga cgcggtgttc
     7621 ggtcctgaac ctgtcggcga cctgctgcgc gaactgcgcc tcgttgaagc gcggatcgtc
     7681 gaaggcgatc gagcaggtct tgaccggctc gtcggacagg ccggccatca tcgcgacgac
     7741 ggcgctcgaa tcgacgccgc cggacagaaa cgcgccgagc ggcacttccg acatcagccg
     7801 gatgcgcgtc gcctcgcgca ggcgttcgat cagctcggcg ccggcgtcct cgaccgacat
     7861 cgccggcagc ggcgcgaacg gtgcgtccca gtaggacttc ggcgccggca gcggctcgcc
     7921 gcggcgcagg cacagcgtgt gcgccggtgg cagcttcagc gccgcgcgat agatcgagcg
     7981 cggatcggga atgtagccgt aggcgaagta gtcctcgacc gcgagcggat cgagcgcgcg
     8041 cgggaagcgc ggatgcgcgg cgaggatctt cagctccgag ccgaagatca gctggccgtc
     8101 cggcagcagc gcgtagtgca gcggcttctt gccgagccgg tcgcgggcca ggaacagggt
     8161 ttcggtgttg cgatcccaca gcgcgaacgc gaacatgccg cgaaagcgcg tgacgcaggc
     8221 ctcgccccac tgctcccagg catgaacgat gacttccgta tcgctatgcg tgcggaacgt
     8281 atgcccgagc gcctgcagtt cggcggcgat gccctggtag ttgtagatct cgccgttgaa
     8341 gacgacgacg accgtgtggt cctcgttcca gagcggctgc tggccgctcg acagatcgat
     8401 gatcgacagc cgccggtgcg cgaggccgac cccgggctcg atgtgcacgc cgccctcgtc
     8461 ggggccgcgg tggaactgga cctggttcat cgacgcgagc acgtctgcct cgatgttccg
     8521 ccgttcgcgc aagtcgacga tgcctgcgat tccgcacatg gtgcgctgcc actccgggtt
     8581 cgtgttgggt cattcgaagg cgtgatccct cgccgacggc ctgcacgcag cgatccgcag
     8641 ccggaaaagc aggccggcca tcgggattcc ctgtcgttgc ggtgccgcat tatgccgggc
     8701 catcccgccg gccgcagtcg gattcggtca acttgcgtca agtgatgccg ctcaggtcca
     8761 cgatgccccg gcctgcgcgg cccgtcacca ctgtgctagc gtgcgcgccc tgcctcagcc
     8821 cccgtccttg atcgccgccc gccgcacatg gatgccctct ggatctgcct cgccttcgct
     8881 gccggcctgc tcgcgcaccg catcggcctg ccgccgctgg tcggctatct cgccgccggc
     8941 ttcggcctgc atgcggccgg cgaacgcagc ggcccggcga tcgagcaggt cgcgcacctc
     9001 ggcgtgctgc tgctgatgtt cacgatcggc ctgaagctca ggctgcagta cctcgcacgg
     9061 cccgagatct ggggcggcgg gctcgcgcac ctcggcctgt tcgcgctgct cgccggcgtg
     9121 ctggtctggc tgctcgccgc gcaggcgccc ggaccggcga ccgccctcgg actcgcgctc
     9181 ggcttttcga gcaccgtgat cgccgccaag tcgctggaag ccaagcagga actgcgctcc
     9241 ttccacggcc gggtcgcgat cggcatcctg atcgtgcagg acctcgccgc ggtcggcctg
     9301 ctgtcggtgg cggccgaggc gccgcacccg ggcgccgccg cgctgctgct gctgccgctg
     9361 gcgcgcccgc tgctgctgaa gctgctgtcg gcctgcggtc atgacgaact gctgctggtg
     9421 ttcggcctcg cgctcgccgc cggcgccggc gcgctgttcc acgcgctcgg gctctcggcc
     9481 gaactcggcg cgctggccgc cggcgcgctg ctcgccgcgc acgggcgcgc cggtgaactc
     9541 gccagcgcgc tgtgggggct gaaggaagcc ttcctgctcg gcttcttttt gcagatcggc
     9601 ctcgccggcg gtccggatgc agcaacgttc ttcggcgcgc tcgggctgct gctgctgctg
     9661 ccgctgaagg ccgcggcact gttcctcgtg ctgatcgcgt tccggctgcg cgcgcgcagc
     9721 gccttcctga gcgcgctgtc gctcgcgagc tacagcgagt tcgcgctgat cgtcgcgcag
     9781 ctcggcgtgc aggccggctg gctcgacgcg cagtggctga cgctgctggc gctggcgatc
     9841 gcggcctcgt tcgcgctcgg cgcgccgctg aaccgcctcg cgcacccgct gttcgagcgc
     9901 tgggaagcgc gcctgctcgg cttcgaacgc cacgtgcgcc accccgacga ggagccgatc
     9961 cacctcggcg ccgcgcatct gctggtgatc ggcatgggcc gcgccggcac cgtcgcctac
    10021 gactacctcg tgcagcacga gcgcgtggtc gggctcgaca gcgacctgtc gcgggtcggc
    10081 gatcacctgc acgccggccg gcgcgtgctc tacgccgatg ccgaggaccc gggcctctgg
    10141 caccacctgc acctgtccgg catccgcgcc gtgctgctga tgatgcgcga cgccgaggcg
    10201 cgcgtgatcg ccgcgcgcca gttgcgccgc cacggcttca ccggcctcgt ggccgcgatc
    10261 agccattacc ccgacgaagc cgccgcggtc accgccgccg gtgccgacat gaccttcacg
    10321 cgcctcgatc aggtcgggct cggtctcgcc gaacacgtgc tcggcgagct gaagcagcgc
    10381 gcggcggcag gcggctgaca tgcacttcgc gacgctgaac ctctgcaacc tcggcgccga
    10441 tgccgccgca tggcggctcg cgcacttcgg ccggctgatc gcgcagacgc tcgccgcgcc
    10501 ggcactgctc gcgctgcagg agctcaagag cgaggcggag ccg
//
LOCUS       DAOQIE010000117        10457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3195_length_10457_cov_6.844645, whole genome shotgun sequence.
ACCESSION   DAOQIE010000117 DAOQIE010000000
VERSION     DAOQIE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_10457_cov_6.844645"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PK144_08890"
     CDS             <1..281
                     /locus_tag="PK144_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65881.1"
                     /translation="DGRGLAAKPRQPDAKAADSGTGHAGEAVQDERMEPARPSRNPHL
                     PAPVALSPMDDLRPPVSSPMDAGCDPVRMWRKAHMPQGQLQLHSSGSS"
     gene            complement(260..1384)
                     /locus_tag="PK144_08895"
     CDS             complement(260..1384)
                     /locus_tag="PK144_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65882.1"
                     /translation="MNIDLAGKNCLGLELVEWPLPIHEVLYEAEQPIVFLTYSSVLCQ
                     PLLAYLADEESEESSIILASASSEKISNLKKGIIGVREALVSDWMWLVRGSHSDGAAK
                     IWAIRESDVPDGYLPVHGTPLLPEHTVVFSARALGDDIALGRMPCSVVSLVADAVRSS
                     LKVLLDHVRAANTEGRPKDAQRKLYDLPVQRLRFSSFEVGLAEPDHGLFRDESMQVVI
                     SSLTRGLKWAQDAAGQVSLGEGDAEELDAILRATLALTPPGSGVVREIEVSGAWLQGR
                     RFRLDRNARLKVSKQLRAHRAEKIVVYKGRIGEVDDDNLSITIRDTSDGVDRKGFLRE
                     EFWDEVRAHYYYSNRVAISGIERNGRLNVTAVVAQDDPDE"
     gene            complement(1374..1730)
                     /locus_tag="PK144_08900"
     CDS             complement(1374..1730)
                     /locus_tag="PK144_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65883.1"
                     /translation="MQLPDNCPPDVASQRELVVYRIVKTNPPTLDDFKTHAQLGLAPH
                     VDYCRRCSLSLFDSYSNAMHRLNLSPKLGAHVAQATLTPAHGLISPVNKAGHMEWWAY
                     KGKVMPDEFTVSDDEY"
     gene            complement(1995..2498)
                     /locus_tag="PK144_08905"
     CDS             complement(1995..2498)
                     /locus_tag="PK144_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65884.1"
                     /translation="MTFDHAAGNGIRSPARFGAAEHDRPEPATAWEAHRNWQESVTMA
                     GNSYKDREDQIVAVGDAWKRYAADKTLAGLTLEQFQQRATECRQALRRVEDLENQLLG
                     ARNERERLFREMKQLIMYIKNAVSGDPSLGPDSSMYEAMGYVRTSDRKSGLKRPRKGE
                     DEETKGS"
     gene            complement(2729..2977)
                     /locus_tag="PK144_08910"
     CDS             complement(2729..2977)
                     /locus_tag="PK144_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65885.1"
                     /translation="MHRTMLVRKCADFQMDVAHFLLNVASLQRKCGHFQFDPAHFQWK
                     RAWFDWKCTRQKAGCADLMQMCAKLVRWQAGEKRADAA"
     gene            3036..>3697
                     /locus_tag="PK144_08915"
     CDS             3036..>3697
                     /locus_tag="PK144_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65886.1"
                     /translation="MVWKQASLICKNANFLWKAGRRIDRDAGFICKDASQRGNGTGFG
                     GPDASFDGPGAACDCRDMRCVCKRVPPVHAGTGFARQRMRRVCKRAGLMGGIANFKAS
                     AAYLHPLAASRIAPAPSRAPFLHRHGSRHRRIRTICGRPGTGMDQNAGYRGKTASEAR
                     KRADKSGNSPPECPETLLFPELVHDLDHGAGHRGRHRCFGDSKMSYRIFSTEHQGQVV
                     SR"
     assembly_gap    3698..3797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3902..4702)
                     /locus_tag="PK144_08920"
     CDS             complement(3902..4702)
                     /locus_tag="PK144_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNF65887.1"
                     /translation="MQLALYQIDAFTDRPFAGNPAAVVPLERWLPDAVLAAIAAENNL
                     SETAFFVPEGEGFRLRWFTPGIEVDLCGHATLASAWVLYERLGWRAARIPFVCASGPL
                     GVRRLEDGRLELDFPARPLLATDAGLAEAVAAALGAQPVEVRSSDTNLFAVFACEADI
                     RALRPDFAALARLPKQGVIVTAPGDHADFVSRYFAPAAGIDEDPVTGSAHCELTPYWA
                     ARLGRDRLHAQQVSKRHGELQVCLDGARVRIAGHAVPYLEGRITLPGD"
     gene            complement(4837..5073)
                     /locus_tag="PK144_08925"
     CDS             complement(4837..5073)
                     /locus_tag="PK144_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HNF65888.1"
                     /translation="MSTIRELSLPLSRSLPSLRELVALPATGMGRIVRTAGLWYRRHN
                     ERRALAALPDYLLKDIGLSDADVWAESRKWFWQE"
     gene            5175..6551
                     /locus_tag="PK144_08930"
     CDS             5175..6551
                     /locus_tag="PK144_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNF65889.1"
                     /translation="MWTPDLNPYAGPRYRAIADALEADLREGRLAPGARLPTHRELAE
                     RLGVTVGTITRAYAEAEARGLTRGTVGRGTFVRETRVQAPVMIHAPAPGLIDLSVNRP
                     ALLPQIRALQQTLTELPATLDLAALLNYAPTCGLPAHRAAGAGFIAMLGLPGYTTEQI
                     VITCGAQHALLAVLMAMAEPGDVLLTEALAYPGLIAAASQARLRVVPVAIDEHGLIPE
                     ALREAAREHRPKLLATVATLHNPTTAVLPAARRAEIAEIAREFDFALLDDDVYGFLVP
                     ERPPALASFAPERSCYVTCTSKALMPGLRIGYVAAPSAWAERISAAVRTSVWMAPPLM
                     AEIAARWIAEGTAERLMDWQREEIRARQQLAAGRLAAHPYRAQPGGFHLWLPLPEPWR
                     EGEFVAAARARGVAVAPAEVFAVGRQPTPPAVRVCVSAQPDRESLAQGLDVLAGLLAQ
                     PPSAPLLM"
     gene            6648..7370
                     /locus_tag="PK144_08935"
     CDS             6648..7370
                     /locus_tag="PK144_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNF65890.1"
                     /translation="MTEALFRDDAYLADCTAIVTAVTRTDTGTTVECDRTVFYALGGG
                     QPGDTGTLTRADGRRLAILDTRKGETPGTIQHWLAADAEPPAVGERVHLALDWTRRHR
                     HMRMHTALHLLSAVIPAGVTGGSVSADKGRLDFDLPDQLLDKADIEARLNALIAGDHA
                     VGTRWISDAELDAQPELVKTLSVQPPRGQGRVRLVGIAGIDLQPCGGTHVARTGEIGR
                     ILVTKIEKKSTHNRRVILGFAD"
     gene            7529..9157
                     /locus_tag="PK144_08940"
     CDS             7529..9157
                     /locus_tag="PK144_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNF65891.1"
                     /translation="MPDDPHVAPSAAVAEALGVVHAYHARSKHRLERYAAGPETLDWS
                     AQPDPFRTYTGAGVQLLPLGADALASRWQAVHRPGAIPPQPLNADTLGTLLELTLGLA
                     AWKQAGPDRWAVRCNPSSGNLHPTEGYVLAQGLPGLADGLWHYRPREHALERRCAWPA
                     PAVSSPRLYVGFSAVLWRELWKYGERAFRYCQLDAGHALGALAYAAAALGWHLRPVSI
                     GHEALAALLGLDRDADFGKAEREEPELLVEVLAGGAASHTLPVIPPDAVWHGQANVLD
                     RWPMYRWPVVDEVARASRRPAGVSEHASPADAALPPLQPAATEVRAATLIRARRSAQR
                     FVRDAQQDLASFLRLLDALLPRTGLPPWSGLNAPVRVHPLLFVHRVEGLAAGVYVLPR
                     SASAVTTLRSALSEGFSWDKPEGVPAHLPLYRLADGTTAKITRTLSCHQALAADCTFT
                     LALLAEFDATLAAAPWAYRELHHEAGLIAQALYLHAEAEGLRGTGIGCFFDDAVHALL
                     GLADARFQSLYHFTVGRPQPDARIASEPPYPDRR"
     gene            9203..10015
                     /locus_tag="PK144_08945"
     CDS             9203..10015
                     /locus_tag="PK144_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF65892.1"
                     /translation="MSDRHYTRLSPAEAETLLATGLASVFDVREKPFFAQDHLPGARH
                     LDDAGMDAALLGTPRRQPVLIYCHHGNASRAVAQAFADFGFTQVSDLAGGWEAWRAHR
                     AAGIQAVRGVAPREPLPAVLAGWLQAEGYPADDLDARLANGTTPLMRAARLGLADRVA
                     DLVIAGAPLESRNADGNTALWLACFSDDADTLDILLNAGASPDNLNDNGATCLMYAAS
                     AGKAGVVQALLAAGADASLRSLDDFTALDMASTEACLNLLRPGRGRRAPGTA"
BASE COUNT         1751 a         3584 c         3322 g         1700 t
ORIGIN      
        1 cagatggcag gggattggca gcgaagccgc ggcagccgga tgcgaaggct gcggattcgg
       61 gaacgggaca tgcgggcgaa gcggtgcagg atgagcggat ggagccagcc cgcccctcgc
      121 gaaacccgca tttacctgcc ccggtcgcgc tgagcccgat ggatgacctg aggccgccgg
      181 tctcaagtcc gatggacgca ggctgcgatc cggtcaggat gtggcgcaaa gcgcacatgc
      241 cgcaaggtca actgcagctt cactcgtccg ggtcgtcctg agcgaccacg gcggtcacat
      301 tcaggcgacc attgcgctcg atgcccgata ttgcaacgcg attggaatag taataatggg
      361 cacgaacctc gtcccagaac tcctcgcgca agaaaccctt gcgatcgacg ccgtcactgg
      421 tatcccggat ggtgatgctc agattgtcat catccacttc accgatccgc cccttgtaca
      481 cgacgatttt ctcggcccga tgcgcccgta actgcttact gactttgagg cgagcattgc
      541 ggtccagacg aaaacgccgg ccctgcagcc aggcaccgga gacttcgatt tcacgcacaa
      601 ccccggaacc tggaggcgtc agcgccagcg ttgcgcgcag gatggcatcc agttcctctg
      661 catccccctc gcccagcgac acctgccccg cagcatcctg cgcccatttc aagccacgcg
      721 tcagagatga gatgacaacc tgcatgcttt cgtccctgaa aagaccatga tcaggctctg
      781 ccaatccaac ttcaaaactg gaaaaacgca atctctgcac cggcaaatca taaagttttc
      841 gttgggcatc cttgggacgc ccctctgtgt tggccgcccg cacatgatca agcagcactt
      901 tgagcgaaga tcgcactgcg tccgcaacca atgaaacgac actacatggc atgcgaccga
      961 gcgcaatatc atctcctaat gcccgcgctg aaaaaacaac ggtgtgttcg ggcaaaagcg
     1021 gagtgccatg aacgggcaga tagccatctg gcacatccga ttccctgatt gcccagattt
     1081 ttgctgcacc atcggaatgc gaaccacgaa ccagccacat ccagtcagaa accagagctt
     1141 ccctgacgcc aatgatgcct tttttaagat tgctgatttt ttctgaggaa gccgatgcca
     1201 gaattatgga actttcttca gactcctcat ccgccaaata agcaagcaat ggctggcaaa
     1261 gcacagaaga gtaagtcaag aatacgattg gctgctcggc ctcatacaaa acctcatgaa
     1321 tcggcaacgg ccactcgacg agttccaacc ccaagcagtt tttgcctgcc agatcaatat
     1381 tcatcatcgc tgaccgtgaa ttcgtccggc atcaccttgc ctttataagc ccaccactcc
     1441 atatgccccg ctttattaac aggcgagatc aggccatgcg ctggtgtcag agttgcctgc
     1501 gcgacatgag cccccagttt cgggcttaaa ttcagacgat gcatcgcatt cgaataacta
     1561 tcaaacaagg acaacgaaca gcgccgacaa taatcgacat gcggcgcaag cccaagctga
     1621 gcatgtgttt tgaaatcatc cagagtcggc gggttggttt ttacgatacg atacacaacc
     1681 aactctcttt gggatgcaac gtccggcgga caattatctg gcaactgcat catgaattcc
     1741 gacacaatcc cgtcctcgca tcgaatatga ttcccttacc acctatataa accacacact
     1801 atagtgtaaa cacatcatga atcaataaca acaaccccca ctcggaacgc ctcggaccat
     1861 atccagacga tccaaaagct cccatgacgc tcctgatttt ggagcacaca aacatgagct
     1921 gaaaaatcaa acggcgcaca cacggcatcc tctgccacgc cgcggtgcgc cgtttccatg
     1981 tcttgtccga gtgtttacga ccccttcgtc tcctcatcct cccccttcct gggacgcttc
     2041 agtccgctct tgcggtcgct cgtgcgcacg tagcccatgg cctcgtacat cgagctgtcc
     2101 gggccgaggc tcgggtcgcc gctgacggcg ttcttgatat acatgatcag ttgcttcatc
     2161 tccctgaaca ggcgttcgcg ctcgttgcgg gcgccgagga gctggttttc gaggtcttcg
     2221 acgcggcgca gggcctgtcg gcattcggtg gcgcgctgct ggaattgctc cagcgtcaga
     2281 ccggccagcg tcttgtcggc tgcatagcgt ttccaggcat cgcccacggc gacgatctgg
     2341 tcttcgcggt ctttgtagct gtttcctgcc atggtgactg actcctgcca gttccggtgc
     2401 gcttcccatg ccgtcgccgg ctccggccgg tcgtgctccg ctgccccgaa gcgcgcaggg
     2461 ctgcgaattc cattgccggc agcatggtcg aaagtcatcg gcctgcaagt cgcgcagccg
     2521 cgccggcggc cggtgcagca ttacgatcgc gtcatgtttt ccggatcaaa ccgcagccat
     2581 ccggccgcgg ccgcgccgga gcggccgcgg cctcgttgcc gtcatttccc tgacatttca
     2641 ataccctgat tccactccgt cccccagccc ctccccgatg aacgctgcag ggctgccagc
     2701 gtcgctgcgg acagccggga tcgattcttc atgccgcgtc agcccgcttt tctcccgcct
     2761 gccaacgcac cagcttcgcg cacatctgca tcaaatccgc acatccggct ttctggcgag
     2821 tacatttcca gtcgaaccat gcccgtttcc actggaaatg cgcaggatcg aactggaaat
     2881 gcccacattt ccgttgcaaa ctcgcaacat tcaacaggaa atgcgcgaca tccatctgga
     2941 aatctgcaca tttccgcacc aacatcgtgc gatgcatgac atgcccgcac catttccatc
     3001 ctcacctggc agggttggaa tggaaatctg cgaagttggt ctggaaacag gcaagcttga
     3061 tctgcaagaa cgcgaacttc ctctggaaag cggggcgtcg tatcgacagg gatgccggct
     3121 tcatctgcaa ggacgcgagt cagcgcggca acggcacggg cttcggcgga ccggacgcga
     3181 gtttcgacgg ccccggcgca gcatgcgatt gcagggacat gcgctgcgtc tgcaagcgtg
     3241 tgccgcctgt ccatgcaggc accggcttcg cccgccagcg catgcggcgt gtctgcaagc
     3301 gcgcaggctt gatgggcggg atcgcgaact tcaaggcttc ggctgcgtat ctgcacccgc
     3361 tcgcagcatc gcgcatcgcg ccggcgccat cgcgcgcacc attcctgcac aggcatggca
     3421 gccggcatcg ccggattcgc acgatctgcg gacggcccgg caccggaatg gatcagaatg
     3481 cgggttatcg gggcaaaacc gcatcggaag cgcggaaaag agcagacaag tccggcaatt
     3541 ccccgccgga atgtccggaa actttacttt ttcccgaact tgtccatgat ctggatcacg
     3601 gcgccggtca tcgtggccgg caccgttgct tcggagacag caagatgagc taccggattt
     3661 tcagcaccga acatcagggt caggtcgttt ccagggannn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnntgc gcggccggtg gcgtttcggg gtccggaaag cctgcggatc
     3841 gatgccggcc ggcagcggct ccgcaacgga cccgaaaccg cgccggccgc gttgaaccgc
     3901 ctcagtctcc gggcagcgtg atccgccctt cgagatacgg cacggcatgg ccggcgatgc
     3961 gcacgcgcgc gccatcgaga catacctgca gttcgccgtg gcgcttcgag acctgctgcg
     4021 cgtgcaggcg atcccggccc agccgtgcgg cccagtacgg ggtcagctcg cagtgggcgg
     4081 agccggtgac cgggtcttcg tcgatgccgg cggccggggc gaaatagcgg ctgacgaagt
     4141 cggcatgatc gccgggggcg gtgacgatga cgccctgctt cggcagacgt gcgagggccg
     4201 cgaagtccgg gcgcagcgca cggatgtcgg cttcgcaggc gaagacggcg aacaggttgg
     4261 tgtcggagct gcgcacctcg accggctgcg cgccgagcgc ggcggcgacg gcttcggcga
     4321 gcccggcgtc ggtggcgagc agcgggcggg cgggaaaatc cagctccagc cgaccgtcct
     4381 ccagccggcg cacgccgagc gggccgctgg cgcagacgaa aggaatgcgc gctgcgcgcc
     4441 agccgaggcg ctcatagagc acccaggcgc tggcgagcgt ggcatggccg cagagatcga
     4501 cctcgatgcc gggcgtgaac cagcgcagcc ggaagccttc gccttcgggc acgaagaagg
     4561 cggtttccga caggttgttc tcggcggcga tcgcggccag cacggcatcg ggcagccagc
     4621 gttcgagcgg cacgaccgcc gccgggttgc cggcgaaggg ccggtcggtg aaggcgtcga
     4681 tctggtacag ggcgagctgc atggcgaagg ctccggggac aagggctgtg cgggcggtca
     4741 cgcgcggttc cggcatcgag ggtgacaatg tgcattgtca ccctgcccgc ggcctgcagc
     4801 gcctgcaaag gccgctgcat cggccgcgcg ccgcgctcat tcctgccaga accacttgcg
     4861 cgattcggcc cagacatcgg catcgctcaa gccgatgtcc ttcagcaggt aatcgggcag
     4921 ggccgccagg gcgcggcgtt cattgtggcg gcgataccag aggccggcgg tgcggacgat
     4981 gcgccccatt cccgtggccg gcagagcgac gagctcgcgc agcgaaggca gggaccggga
     5041 aagaggaaga gacaattcgc ggattgtgct catttttgca tctccagcag caggggcgat
     5101 cactacattg tcaccgcgac atgcagagta gttgacacaa tacggatgtc aagccattct
     5161 ttgtcaccat gacaatgtgg acgccagacc tcaaccccta cgccggcccg cgctaccgcg
     5221 cgatcgccga tgccctcgaa gccgacctgc gcgaggggcg gcttgcgccg ggcgcgcgcc
     5281 tgccgacgca ccgcgagctg gccgagcgcc tcggcgtgac cgtcggcacg atcacgcgcg
     5341 cctatgccga ggccgaagcg cgcggactga cgcgcgggac ggtcgggcgc ggcaccttcg
     5401 tgcgcgagac gcgcgtgcag gcgccggtga tgattcatgc gccggccccg gggctgatcg
     5461 atctgtcggt gaaccgcccg gcgctgctgc cgcagatccg ggcgctgcag cagacgctga
     5521 ccgagctgcc ggccacgctc gatctcgcgg cgctgctcaa ctacgcgccg acctgcggcc
     5581 tgccggcgca ccgcgccgcc ggcgccggct tcatcgcgat gctcgggctg ccgggctata
     5641 cgacggagca gatcgtcatc acctgcggcg cccagcacgc cctgctcgcg gtgctgatgg
     5701 cgatggccga gcccggcgat gtgctgctga ccgaagccct cgcgtatccg gggctgatcg
     5761 cggcggccag tcaggcgcgg ctgcgcgtcg tgccggtcgc gatcgacgag cacgggctga
     5821 tccccgaagc gctgcgggaa gcggcgcgcg agcaccggcc gaagctgctc gcgaccgtcg
     5881 cgacgctgca caacccgacc actgcggtgc tgccggcggc gcgccgggcc gagatcgccg
     5941 agatcgcccg cgagttcgac ttcgcgctgc tcgatgacga tgtctacggc tttctggtgc
     6001 cggagcgccc gccggcgctc gcgagcttcg cgcccgagcg cagctgctac gtgacctgca
     6061 cgtcgaaggc gctgatgccg ggcctgcgca tcggctatgt cgcggcgccg tcggcctggg
     6121 ccgagcgcat cagtgcggcc gtgcgcacct cggtgtggat ggcaccgccg ctgatggccg
     6181 agattgccgc gcgctggatc gccgagggca cggccgagcg cctgatggac tggcagcgcg
     6241 aggagatccg cgcgcgccag cagctcgcgg ccgggcggct ggccgcgcat ccgtaccgcg
     6301 cgcagcccgg gggcttccac ctgtggctgc cgctgccgga gccgtggcgc gagggcgagt
     6361 tcgtcgccgc ggcgcgcgcg cgcggggtcg ccgtggcgcc ggccgaggtg ttcgcggtcg
     6421 ggcgtcagcc gacaccgccg gcggtgcgcg tctgcgtcag cgcccagccc gatcgcgaat
     6481 cgctggcgca ggggctggac gtgctcgccg gcctgctggc gcagccgccg tcggcaccgc
     6541 tgctgatgtg aagacacggc atcggcccgg acttcgacgc atcctgccgg tgtttcgctc
     6601 gatcccgccc gtcccttgac gattcttgcc tgccggagcc tgccgccatg accgaagcgc
     6661 tgtttcgcga cgacgcctac ctcgccgact gcacggcgat cgtcaccgcc gtgacgcgca
     6721 ccgacacggg gacgacggtc gaatgcgatc gcaccgtgtt ctacgcgctc ggcggcggtc
     6781 agcccgggga taccggcacg ctgacgcggg ccgacggccg ccggctggcg atcctcgata
     6841 cgcgcaaggg cgaaaccccc ggcacgatcc agcactggct cgccgccgat gccgagccgc
     6901 ccgccgtcgg cgagcgcgtg catctggcgc tcgactggac gcgccggcac cggcacatgc
     6961 gcatgcacac ggcgctgcat ctgctgtcgg cggtgatccc ggccggcgtc accggcggct
     7021 cggtcagcgc cgacaagggc cggctcgact tcgatctgcc cgatcagctg ctcgacaagg
     7081 ccgacatcga ggcgcgcctg aacgcactga tcgccggtga tcacgcggtc ggcacgcgct
     7141 ggatcagcga tgccgaactc gatgcgcagc cggagctggt gaagacgctg tcggtgcagc
     7201 cgccgcgcgg tcagggccgc gtgcgcctgg tcgggatcgc cggcatcgac ctgcagccct
     7261 gcggcggcac gcacgtcgcg cgcaccggcg agatcggccg catcctggtc acgaagatcg
     7321 agaagaaatc cacgcacaac cgccgcgtga tcctcggctt cgcggactga caccgcgccc
     7381 gcgactgtcg gcaagcgcac gccgcgtcgg ctttgtcgga tatccgtgca tgccggcagg
     7441 actccatctg catgatcctc aagcgttttg tccggatggc gcactcctcg catgctgcag
     7501 cgcacattcc tgacggaccg ctgccgccat gcccgacgac ccgcacgtcg ccccttccgc
     7561 tgccgtcgcc gaagccctgg gcgtggtgca cgcctatcac gcgcgcagca agcaccggct
     7621 ggagcgctac gccgccggcc cggagacgct ggactggagc gcgcagcccg atccgttccg
     7681 cacctacacg ggcgccggcg tgcagctgct gcccctcggc gccgatgcgc tcgcgagccg
     7741 ctggcaggcc gtgcaccggc ccggcgcgat cccgccgcag ccgctgaacg ccgatacgct
     7801 cggcacgctg ctcgaactga cgctcggcct cgcggcctgg aagcaggccg gaccggatcg
     7861 ctgggcggtg cgctgcaatc cgtcgagcgg caacctgcat ccgaccgagg gctacgtgct
     7921 cgcgcaaggc ctgccgggcc tcgccgacgg cctctggcac taccggccgc gcgagcacgc
     7981 gctggagcgg cgctgcgcgt ggccggcccc ggcggtctcg tcgccacgtt tatatgtggg
     8041 cttcagcgcc gtgctgtggc gcgaactctg gaagtacggc gaacgcgcct tccgctactg
     8101 ccagctcgat gccggccacg cgctcggcgc cctcgcctat gccgcggccg cgctcggctg
     8161 gcacctgcgg ccggtgtcga tcggacacga ggcactggcg gcactgctcg gtctcgatcg
     8221 cgacgccgac ttcggcaagg ccgagcgcga ggagccggaa ctgctggtcg aggtgctggc
     8281 cggtggcgct gcgtcgcaca cgctgcccgt catcccgccg gacgccgtct ggcacgggca
     8341 ggccaatgtg ctcgatcgct ggccgatgta ccgctggccg gtggtcgatg aggtcgcccg
     8401 cgccagccgc cggccggccg gcgtgtcgga gcacgcatcg ccggcggatg ccgcactgcc
     8461 gccgctgcag cccgctgcga ccgaggtccg cgccgcgacg ctgatccgcg cacggcgcag
     8521 cgcacagcgc ttcgtgcgcg acgcgcagca ggatctcgct tccttcctgc gcctgctcga
     8581 tgcgctgctg ccgcgcaccg gcctgccgcc gtggagcggg ctgaatgcgc cggtgcgcgt
     8641 gcatccgctg ctgttcgtgc accgcgtcga ggggctggcg gcaggcgtct acgtactgcc
     8701 ccgcagtgcg tccgccgtga cgacgctgcg ctcggcgctg tcggagggct tcagctggga
     8761 caaacccgag ggtgtgccgg cgcatctgcc gctgtaccgg ctcgccgatg gcacgaccgc
     8821 gaagatcacg cgcacgctga gctgccatca ggcgctcgcg gccgactgca cgttcacgct
     8881 ggcgctgctc gccgagttcg atgccacgct cgccgccgcc ccctgggcgt atcgcgaact
     8941 gcatcacgag gccgggctga tcgcgcaggc gctgtacctg cacgccgagg ccgagggcct
     9001 gcgcggcacc ggcatcggct gcttcttcga tgacgccgtg cacgcgctgc tcgggctcgc
     9061 ggatgcgcgc ttccagtcgc tgtaccactt cacggtcggc cggccgcagc cggatgcgcg
     9121 catcgccagc gagccgccgt atcccgaccg ccgctgattc ccctgtcccg cccctttccg
     9181 cctgccccgg agcctgctgc cgatgagcga tcgccattac actcgcctga gcccggccga
     9241 agccgaaacc ctgctcgcca ccggcctggc cagcgtcttc gacgtgcgcg agaagccgtt
     9301 cttcgcgcag gatcatctgc ccggcgccag acacctcgac gatgccggca tggatgccgc
     9361 gctgctcggc acgccgcgtc gccagccggt gctgatctac tgccatcatg gaaacgccag
     9421 ccgcgcggtc gcgcaggcct tcgcggactt cggcttcacg caggtcagcg atctcgccgg
     9481 cggctgggaa gcctggcgtg cgcaccgggc ggcgggcatc caggcggtgc gcggcgttgc
     9541 cccgcgcgaa ccgctgccgg cggtgctcgc cggctggctg caggccgaag gctatccggc
     9601 ggatgatctc gatgcgcgac ttgccaacgg cacgacgccg ctgatgcgcg ccgcgcgcct
     9661 cggactggcc gatcgggtcg ccgatctggt gatcgccggt gcaccgctcg aatcgcgcaa
     9721 tgccgacggc aacaccgcgc tgtggctcgc ctgcttcagc gacgacgccg acacgctcga
     9781 catcctgctg aacgccggcg ccagccccga caacctcaac gacaatggcg cgacctgcct
     9841 gatgtacgca gcctcggccg gcaaggccgg ggtcgtgcag gcgctgctcg ccgccggggc
     9901 cgacgccagc ctgcgctcgc tcgatgactt cacggcgctg gacatggcct cgaccgaagc
     9961 ctgcctgaac ctgctgcgcc ccggacgcgg acggagagcc ccgggcacgg cctgaggcgg
    10021 gcatcgttgc ggtccgcgat ggtgcaatgc agcgttaaaa atcctgacac tcaccgagcg
    10081 gcctgcgcac ggcaagcctg cgacgagctc cgccttagcg gccagccgcc ctcaggccct
    10141 tcaatccgct tcaacacctc aaggccctga tccggagcgt tttcctgtaa ccggatcgtg
    10201 gcgggaaacg tagggtagag aagcgccatc gcttcgacca ttgtcgaaag atgtcgccac
    10261 gccatggctc gcaacttgct ttgcagaatg cgtcagcgcc cggctcaaga agcgggccca
    10321 gcttccgacg tttaccgtca gcagccatca acttcccgcc gcgcatggcg aggaagccag
    10381 ctagtgtcgc gcctgggtac agggaaagta ccggccgtga caggaatgtt tgaggtgtta
    10441 gcgatgcgcc tcccggc
//
LOCUS       DAOQIE010000118        10454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3196_length_10454_cov_6.838350, whole genome shotgun sequence.
ACCESSION   DAOQIE010000118 DAOQIE010000000
VERSION     DAOQIE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3196_length_10454_cov_6.838350"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1413)
                     /locus_tag="PK144_08950"
     CDS             complement(151..1413)
                     /locus_tag="PK144_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNF65893.1"
                     /translation="MNAPTALPAGNGATLSDPDDPVVITGTGLVSALGCDAAAALASI
                     AAGGSGIATVESFTRPARAALVREFTPTDYVAKAKLRRMDPLNVFAIGAATQALAMAG
                     LDEAGRLGCGAVVGSGFAGYRSVVEHQKKLVRDGIRTLSPIHFPTTVYNATAGLLAIE
                     LQVLGPNSTVTGVDASGEHALYYAATLLARGQCEQILAIGADELSPALFDGFDALGLL
                     ERDTTRSPLPFGRERHGFAPGEGAAALLLERESTARARGATILGRLLGFGFANGASTP
                     FAHNPDPALIATAATEALGRARLTAGQIDWCSASACGSPTRDAAEAAGLASLFAAHGT
                     PLRALKAWTGEYAASGIQRLLLGLHAARLGALPAMPAGDYLPEVAPLLAADCAAPRRC
                     LHLGTGVGGNTVAIVVEAAATGRDREAA"
     gene            complement(1410..2207)
                     /locus_tag="PK144_08955"
     CDS             complement(1410..2207)
                     /locus_tag="PK144_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF65894.1"
                     /translation="MAKAAHRAPAAGARVLGLALGTRLTLRAGATLVVDGDSRIVVDP
                     GPFQSLAELAAALQPHGLTPADIDTVYFTHLHFDHYTPAAIPPGVRRVCIPAAERAFI
                     DALTPLAADLDAYRARLLETHERIAPVFLREFVRLARDARYDFARLPYADRLVELGPD
                     ARPSAHTRTVALPGHCPGQLGLEVTTAHGTVLIAGDAVLSLADWQAPDVSHHLIVHER
                     AALIASRERAGRADCVVPGHGDWFSPRSGRVLAADSDLIASTGVATA"
     gene            complement(2200..2973)
                     /gene="fabG"
                     /locus_tag="PK144_08960"
     CDS             complement(2200..2973)
                     /gene="fabG"
                     /locus_tag="PK144_08960"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNF65895.1"
                     /translation="MNTAAAPVPASAPAPVALVTGASRGIGRAIATALAASGHRVAVN
                     YRSRADEAESLVAGITAAGGEAAAFAADVADPQQVDAMVAAVRTHFGAPVAVLVNNAG
                     LLRDGFLAMMPETAWDAVLDTNLRGAFLVTRAVVRDMMRARAGTIVNVASVSGVIGTP
                     GQANYAASKGGLIALTRSNAQELARYGIRVNAVAPGFIETEMLTEMNPRQLDAALSRV
                     PMGRAGKPEEVGAVVAFLASPAASYVTGQTLVIDGGLCG"
     gene            complement(2970..4181)
                     /locus_tag="PK144_08965"
     CDS             complement(2970..4181)
                     /locus_tag="PK144_08965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNF65896.1"
                     /translation="MQRIAITGIGIVSPIGNDTELFEAALRLGRSGVGPNDWHDTEGY
                     GSHRIGRVRDFTPPAAAGRFERRHLSTVDQYALAATAEALGRAGLDAEARRSLRIGVV
                     FGSGGSVENVENYVFSALAERPLRPSTLLPLSPDNAGGAVAAHYGLRGPRSSIMTACS
                     SGAAAVGYGADLLREGHADVMLVGGMEALSYVTLSGFNALGALSPTINRPFDLNRDGI
                     VIGEAAAVLVLEPLERAKARGAHVFAEFVSYGLAADGNHITAPSPEGDGMARAMRTAL
                     RLGGLAPEAIAYINAHGTGTALNDASETAAIRRTFGAHADRLCLSSIKPMIGHTLSAA
                     GAIEAAATVLSLAGQFAPPTLNYETPDPACDLDVVPNVARNLDMEYAMSNSLAFGGNN
                     ASLIFRRAEAA"
     gene            complement(4226..4495)
                     /locus_tag="PK144_08970"
     CDS             complement(4226..4495)
                     /locus_tag="PK144_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNF65897.1"
                     /translation="MNLDNIKTGLKEIFAERLNLDVAGVDAGDDDKLFSDDGWGLDSV
                     DVIDIVLGVEQKFGVAIRADDDVKVHFSTLNTLAAYIGEQMKAAA"
     gene            complement(4538..6454)
                     /locus_tag="PK144_08975"
     CDS             complement(4538..6454)
                     /locus_tag="PK144_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF65898.1"
                     /translation="MSALLDGCTAIARGAWEGGASFAAGYPGSPVTGVLDATAGYPEI
                     RRQWAANEKIALETALGAAWSGARALVVMKHVGLNVAADPLYNAPYTGVRGALVIVVG
                     DDPGASSSQNEQDTRLVAKAAGVPVLEPSSVAEALVFTRLAFEISARHDLPVIVRVTT
                     PIAYGSTGAVAGARWPVPAAAGHARPIQKYLLLPAFVPARHTALIERLSALGASPAAR
                     WFCETSLPPAAAAGTRHPVGIVCAGFPAAAVRELCADTVPVLTLGLVHPLPEAPIRAF
                     AQHCERLLVVEESSHYLEDAIRALGLPVARRSHYDGVGSFGPKQLLSDEAPVVNALVQ
                     PLIDAAEQRRRHIPILAPRTPGFCAGCSHRGIFSLLAKRDLYVVGDIGCNTLGAIQPY
                     GALHANLCMGASISVLQGYLAALGPDAAQRTIAVLGDSTFFHSGLPAILSAVHNGLPG
                     TVLVLDNAGSAMTGHQQTAHARDAEGWRTLLGALGVPDVRVVDALDLGELDAALDAAL
                     ATDALSVVVLKGDCVQSLPRKGPTNYRYTVRTDACSGCGDCLKVDCPAIVQLAASAAG
                     GPRKVAITHECIGCGLCSQTCPEHAIVPRTVQTVSPRLARLLAPLPWRRLIRGLRGVA
                     PVRRLLDRFETETY"
     gene            complement(6451..7359)
                     /locus_tag="PK144_08980"
     CDS             complement(6451..7359)
                     /locus_tag="PK144_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNF65899.1"
                     /translation="MSVVLMFPGLGSEYPELLARQCAEDPQAAERLADWRERLGVPLT
                     DTPPADPRARELARQMAIHAVNLLAWERHGAAYPQAAVCGHSLGFYAAAVAAGALDAD
                     ASFAWIGAVFERAWARFEHDRDCVYVLTARDTLDGPALAAAWGVELLAVNGPCQIVVY
                     GAPAVLEALCAALAGRLVRHGPLGSCCPFHSVRMAPVCVEVAHWLATSGLQPRAPQRP
                     LWSHLDATRLMDAEAVRATLITQPQLRVRWDALIGRLAADGHDTFVELGPNRILAQAA
                     RWIVPGSLTVLSTDSARRIRRAGSPA"
     gene            complement(7377..8141)
                     /locus_tag="PK144_08985"
     CDS             complement(7377..8141)
                     /locus_tag="PK144_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65900.1"
                     /translation="MHAPAARTEALHAGRQRPHADLPAGPVLHQPSAHGPRSSLSVCV
                     AGLGGLGATTLNARLAELLRTSHGEVCAIDQLGVARRRASVTNLLRAGRGVRCAGWCG
                     SPYDVLIALEESESLRQLDRIGPQTLVLLADCRVETIAGYSGAPLPSHAVVRSTLDQH
                     AGYVLQLPVTRELGRHQLNGAHGTALFFGAFAALLGLTVAEAEHHVSTGHARGAATLL
                     ANTGHAAAVRAGLLGWLNGGHRQHAATAVVTPAVAA"
     gene            8669..9541
                     /locus_tag="PK144_08990"
     CDS             8669..9541
                     /locus_tag="PK144_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF65901.1"
                     /translation="MSADHRRRSGLLLVLAAAAGFGLMAVFARSAYAAGVNPPTLLAL
                     RFTLAALLLVAIVQARARPWPRGRALIGLIAMGGGFYTLIAQCYFVALTQASAALVAL
                     VLYTFPVLVMAINVACFGERLTVAKCGAALLAMAGLALTIGGELHGSAGGIALALVAA
                     AGYAAYILIGDRVARGVDPLCGGAVVIAASAVTSALLALAEGVTLPQTGGGWLAVTGI
                     ALVSTVFAMTAFLEGLSRVGSTTAAMASTFEPVVTVASAAWLLGETVSPARLAGGGLI
                     LAAVVLLARAPHRP"
     gene            9583..>10454
                     /locus_tag="PK144_08995"
     CDS             9583..>10454
                     /locus_tag="PK144_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNF65902.1"
                     /translation="MTMRPRRSVLYMPGSNARALDKARSLDVDALILDLEDSVAPEAK
                     GLGREQIAAALTEGGYGHREVIVRVNALSTPWGHDDVRALAKLGADAFLFPKIDSPAQ
                     VIEAVTALDAAGAPSGTGVWIMAETPLGILNIAAIAACSPRLRCIVLGTSDLAKDLRA
                     RHSPGRIAFVTSLGLCVLAARASGLDALDGVHLDLDDEAGLAAVCEQGRDMGFDGKTL
                     IHPRQIAAANRAFAPSAAEIGEARAIIHGWTQAAAEGKGVCRVNGKLVEHLHVIEATR
                     IVALAEAIAARDALA"
BASE COUNT         1417 a         3655 c         3997 g         1385 t
ORIGIN      
        1 cggcgtcgag ccggacctcg ccgcggccgc ggcggccggg ctcgtcgatg acgacgcgcc
       61 ccggcagcag gaagggcggg cggtgcggca ggcgggcgcg gatgtcggcg gcgccgaatt
      121 cgagcggcgc gcggcactcg gcgatggcgc tcatgcggcc tcccggtcgc ggccggtcgc
      181 ggccgcttcg acgacgatcg cgacggtgtt gccgccgacg ccggtgccga ggtgcaggca
      241 gcggcgcggc gcggcgcagt cggcggcgag cagcggcgcg acctcgggca ggtagtcacc
      301 ggccggcatc gccggcagcg caccgaggcg cgccgcgtgc aggccgagca gcaggcgctg
      361 gatgccggag gccgcgtact cgccggtcca ggccttcagc gcgcgcagcg gcgtgccgtg
      421 cgccgcgaac agcgacgcga gcccggcggc ctcggcggca tcgcgcgtcg gcgagccgca
      481 ggccgaggcc gagcaccagt cgatctggcc ggccgtgagc cgggcgcgcc cgagcgcttc
      541 ggtggcggcg gtcgcgatca gcgccggatc ggggttgtgc gcgaacggcg tcgaggcgcc
      601 gttggcgaag ccgaagccga gcagccggcc gaggatggtc gcgccgcgcg cccgcgccgt
      661 gctctcgcgc tccagcagca gcgccgccgc gccttcgccg ggcgcgaaac cgtggcgctc
      721 gcgcccgaac ggcagcggcg agcgcgtggt gtcgcgttcg agcaggccga gcgcgtcgaa
      781 gccgtcgaac agcgccggcg acagctcgtc ggccccgatc gcgaggatct gctcgcactg
      841 gccgcgcgcg agcagcgtcg ccgcgtaata cagcgcgtgc tcgccgctcg catcgacgcc
      901 ggtgacggtc gagttcgggc cgagcacctg cagctcgatc gcgagcagcc cggccgtggc
      961 gttgtagacc gtggtcggga agtggatcgg gctcagcgtg cggatgccgt cgcgcacgag
     1021 cttcttctga tgctcgacga cgctgcggta gccggcgaag cccgagccga ccacggcgcc
     1081 gcagcccagg cggcctgcct cgtcgagccc ggccatggcc agcgcctgcg tggcggcgcc
     1141 gatcgcgaac acgttcagcg ggtccatgcg ccgcagcttg gccttggcga cgtaatcggt
     1201 cggcgtgaac tcgcgcacca gcgccgcacg cgccggccgc gtgaagctct cgaccgtcgc
     1261 gatgccgctg ccgccggcgg cgatgctcgc gagcgccgcc gcggcgtcgc agccgagcgc
     1321 gctgaccaga ccggtgccgg tgatgacgac cggatcgtcg gggtcactga gggtcgcgcc
     1381 gttgccggcg ggcaatgcag tcggggcgtt catgcggtgg ccactcccgt ggatgcgatg
     1441 aggtcggaat cggccgcgag cacgcggccg ctgcgcggcg agaaccagtc gccgtgaccg
     1501 ggcacgacgc agtcggcccg gccggcgcgc tcgcgcgagg cgatgagcgc cgcgcgctcg
     1561 tggacgatca ggtgatgcga gacatccggc gcctgccagt cggcgagcga cagcacggcg
     1621 tcgccggcga tcagcaccgt gccgtgcgcg gtcgtcacct ccaggccgag ctggccgggg
     1681 cagtgaccgg gcagcgccac ggtgcgcgtg tgcgcgctcg gccgggcgtc cggaccgagt
     1741 tcgaccaggc gatcggcgta cggcagccgc gcgaagtcgt agcgcgcgtc gcgggcgagg
     1801 cgcacgaact cgcgcaggaa caccggcgcg atgcgttcgt gggtttcgag cagccgggcg
     1861 cggtaggcgt cgaggtcggc ggcgagcggc gtcagcgcgt cgatgaaggc gcgctcggcc
     1921 gccgggatgc agacccggcg cacgcccggc gggatggccg ccggcgtgta gtgatcgaag
     1981 tgcaggtgcg tgaagtacac cgtgtcgatg tcggccggcg tcagcccgtg cggctgcagg
     2041 gccgcggcca gctcggcgag gctctggaac gggccggggt cgacgacgat gcgcgagtca
     2101 ccgtcgacga ccagcgtcgc gccggcgcgc agcgtcaggc gggtgccgag cgccagcccg
     2161 agcacgcggg cgccggcggc cggggcgcga tgcgcagcct tagccacaga gccccccgtc
     2221 gatcaccagc gtctggccgg tcacgtagct cgcggccggc gaggcgagga aggcgacgac
     2281 ggcgccgact tcctccggct tgccggcacg gcccatcggc acgcgcgaca gtgccgcatc
     2341 gagctggcgc ggattcatct ccgtgagcat ctcggtctcg atgaagcccg gcgcgacggc
     2401 gttgacgcgg atgccgtagc gcgccagctc ctgcgcgttc gagcgcgtca gcgcgatcag
     2461 cccgcccttc gaggccgcgt agttggcctg gcccggcgtg ccgatgacgc cgctgaccga
     2521 ggcgacgttg acgatggtgc cggcgcgcgc ccgcatcatg tcgcgcacga cggcgcgcgt
     2581 gaccaggaag gcgccgcgca ggttggtgtc gagcacggca tcccaggcgg tctccggcat
     2641 catcgccagg aagccgtcgc gcagcaggcc ggcgttgttg accagcaccg cgaccggcgc
     2701 gccgaagtgc gtgcgcaccg cggcgaccat ggcatcgacc tgctgcgggt cggcgacatc
     2761 ggccgcgaag gccgcggcct cgccgccggc ggccgtgatc ccggcgacca gcgattcggc
     2821 ctcgtcggcg cggctgcggt agttgacggc gacacggtgg ccgctggcgg cgagcgccgt
     2881 ggcaatggcg cggccgatgc cgcgcgaggc gccggtgacg agcgccaccg gcgcgggggc
     2941 ggaagcggga acgggagcgg cggcggtgtt catgcggcct ccgcgcggcg gaagatcagc
     3001 gaggcgttgt tgccgccgaa ggcgagcgag ttggacatcg cgtactccat gtcgaggttg
     3061 cgcgcgacgt tcgggacgac gtcgaggtcg cacgcggggt cgggggtttc gtagttcagc
     3121 gtcggcggcg cgaactggcc ggccagcgac agcacggtcg ccgcggcctc gatggcaccg
     3181 gcggccgaga gcgtgtggcc gatcatcggc ttgatcgaac tgaggcagag gcggtcggcg
     3241 tgggcgccga aggtgcgccg gatcgcggcg gtctcggagg catcgttgag cgccgtgccg
     3301 gtgccgtggg cgttgatgta ggcgatcgcc tcgggcgcga gaccgccgag gcgcagcgcc
     3361 gtgcgcatcg cccgcgccat gccgtcgcct tccggcgagg gcgcggtgat gtggttgccg
     3421 tcggcggcca ggccgtagct gacgaactcg gcgaacacgt gcgcaccgcg cgccttcgcc
     3481 cgctccagcg gttcgagcac cagcaccgcc gccgcctcgc cgatgacgat gccgtcacgg
     3541 ttgaggtcga acggccggtt gatggtcggc gacagcgcgc cgagggcgtt gaagcccgac
     3601 agcgtcacgt aggacagcgc ctccatgccg ccgaccagca tcacgtcggc gtgaccctcg
     3661 cgcagcaggt cggcgccgta gccgaccgcc gcggcgcccg aggagcacgc ggtcatgatg
     3721 ctggagcgcg ggccgcgcag gccgtagtgc gcggcgaccg cgccgccggc gttgtccggc
     3781 gacagcggca gcagcgtcga gggccgcagc ggccgttcgg cgagcgccga gaacacgtag
     3841 ttctcgacgt tctcgacgct gccgccgctg ccgaacacca cgccgatgcg caggctgcgg
     3901 cgcgcttcgg cgtcgagccc ggcacgcccg agcgcctcgg cggtcgccgc cagcgcgtac
     3961 tggtcgacgg tcgacaggtg acggcgctcg aagcgtccgg cggcggccgg cggcgtgaag
     4021 tcgcgcacgc gaccgatccg gtgcgagccg tagccttccg tgtcgtgcca gtcgttggga
     4081 ccgacgccgg agcgcccgag gcgcagggcg gcctcgaaca gctccgtgtc attgccgatc
     4141 ggcgagacga tgccgatgcc ggtgatcgcg atgcgctgca tgggcgtgga ctccgtggcg
     4201 ggggcgggca gagggcggcg ggaactcagg ccgcggcctt catctgctcg ccgatgtagg
     4261 cggcgagcgt gttcagcgtg ctgaagtgca ccttcacgtc gtcatcggcc cggatcgcga
     4321 cgccgaactt ctgctcgacg ccgagcacga tgtcgatcac gtcgacggaa tcgaggcccc
     4381 agccgtcatc ggagaacagc ttgtcgtcgt caccggcatc gacgccggcc acgtcgaggt
     4441 tcaggcgctc ggcaaagatt tccttcaggc cggtcttgat gttgtcgagg ttcatggcgg
     4501 actcctgcaa ttgcacttgg gcggtggtgg ggacgggtca gtaggtttcg gtttcgaagc
     4561 ggtcgagcag gcggcgcacc ggggcgacgc cgcgcagacc gcggatcagg cgccgccagg
     4621 gcagcggcgc cagcaggcgg gcgagccgcg gggagaccgt ctgcacggtc cgcggcacga
     4681 tcgcgtgctc ggggcaggtc tgcgagcaga gcccgcagcc gatgcattca tgggtgatgg
     4741 cgaccttgcg cgggccgccg gcggccgagg ccgcgagctg cacgatcgcc gggcagtcga
     4801 ccttcaggca gtcgccgcag ccgctgcagg cgtcggtgcg caccgtgtag cggtagttgg
     4861 tcgggccctt gcgcggcagg ctctgcacgc agtcgccctt gagcacgacc accgacagcg
     4921 catcggtggc gagcgcggca tcgagtgcgg catcgagttc gccgagatcg agcgcatcga
     4981 cgacgcgcac gtccggcacg ccgagcgcgc cgagcagcgt gcgccagccc tcggcgtcgc
     5041 gggcatgggc ggtctgctgg tggccggtca tcgccgagcc ggcgttgtcg agcaccagca
     5101 ccgtacccgg caggccgttg tgcaccgcgc tcaggatcgc cggcaggccg gagtggaaga
     5161 aggtcgagtc gccgagcacc gcgatcgtgc gctgcgcggc gtcggggccg agcgcggcca
     5221 gatagccctg cagcacgctg atgctcgcgc ccatgcagag attcgcgtgc agcgcgccgt
     5281 agggctggat cgcgccgagc gtgttgcagc cgatgtcgcc gaccacgtac aggtcgcgct
     5341 tcgcgagcag cgagaagatg ccgcggtgcg agcagccggc gcagaagccg ggcgtgcgcg
     5401 gcgcgaggat cgggatgtgc cggcggcgct gctcggcggc atcgatcagc ggctgcacca
     5461 gcgcgttgac gaccggcgcc tcgtcgctga gcagctgctt cgggccgaag ctgccgacgc
     5521 cgtcgtaatg actgcggcgg gcgaccggca ggccgagcgc gcggatcgca tcctccaggt
     5581 agtgcgagct ttcctcgacc accagcaggc gctcgcagtg ctgcgcgaag gcgcggatcg
     5641 gtgcttccgg cagcggatgc acgaggccga gcgtcagcac cggcacggtg tcggcgcaga
     5701 gttcccgcac cgccgcggcc gggaagccgg cgcagacgat gccgaccggg tggcgcgtgc
     5761 cggccgcagc cgccggcggc agcgaggtct cgcagaacca gcgcgcggcg ggactggcgc
     5821 cgagcgccga cagccgctcg atcagcgccg tgtgccgcgc cggcacgaag gccggcagca
     5881 gcaggtactt ctggatcggc cgcgcatggc cggcggcggc cggcaccggc cagcgcgccc
     5941 cggcgaccgc gccggttgag ccgtaggcga tcggcgtggt cacgcgcacg atcaccggca
     6001 ggtcgtggcg ggcgctgatc tcgaaggcga ggcgcgtgaa caccagcgcc tcggccaccg
     6061 acgagggctc cagcaccggc acgccggcgg ccttggcgac caggcgcgta tcctgctcgt
     6121 tctgcgagga cgaggcgccc ggatcgtcgc cgacgacgat gaccagcgca ccgcgcacgc
     6181 cggtgtacgg cgcgttgtag agcgggtcgg cggcgacgtt gaggccgacg tgcttcatga
     6241 ccaccagcgc acgcgcaccg gaccaggcgg caccgagggc ggtttccagc gcgatcttct
     6301 cgttcgccgc ccactggcgg cggatctccg ggtagccggc cgtggcatcg agcacgccgg
     6361 tcaccggcga acccggatag ccggccgcga agctggcgcc gccttcccat gcgccgcggg
     6421 cgatggccgt gcagccgtcg agcagggcac tcatgcggga ctcccggcgc gccggatgcg
     6481 gcgcgcgctg tcggtggaaa gcacggtgag cgaacccggc acgatccagc gcgcggcctg
     6541 cgcgaggatg cggttcgggc cgagttcgac gaaggtgtcg tgaccgtcgg cggcgagccg
     6601 cccgatcagc gcgtcccagc gcacgcgcag ctgcggctgc gtgatcagcg tcgcgcgcac
     6661 ggcttcggca tccatgagcc gcgtggcgtc gaggtgcgac cagagcgggc gctgcggcgc
     6721 acgcggctgc aggccgctcg tggcgagcca gtgcgcgacc tcgacacaca ccggcgccat
     6781 gcgcaccgag tggaaggggc agcacgaacc gagcgggccg tggcgcacga ggcgtccggc
     6841 gagggccgcg cacagggctt cgagcaccgc cggcgcgccg tagacgacga tctggcaggg
     6901 gccgttgacg gcgagcagct ccactcccca ggccgcggcg agcgccggac cgtcgagcgt
     6961 gtcgcgggcg gtcagcacgt acacgcagtc gcggtcgtgc tcgaagcgcg cccaggcccg
     7021 ctcgaacacc gcgccgatcc acgcgaacga ggcgtcggca tcgagcgcac cggcggcgac
     7081 cgccgcggcg tagaagccga ggctgtggcc gcagaccgcg gcctgcggat acgccgcgcc
     7141 gtggcgctcc caggcgagca ggttcacggc gtggatcgcc atctgccggg ccagctcgcg
     7201 tgcgcgcggg tcggccggcg gcgtgtcggt cagcggcacg ccgaggcgct cgcgccagtc
     7261 ggcgaggcgt tcggcggcct gcgggtcctc ggcgcactgg cgcgcgagca gttccggata
     7321 ttcggaaccg aggccgggaa acatcagcac gacggacatc gcgggcgctc cggggctcag
     7381 gccgcaacgg ccggcgtgac gacggccgtc gccgcgtgct ggcggtgacc gccgttcagc
     7441 cagccgagca ggccggcgcg cacggccgcc gcgtggccgg tgttggcgag cagcgtcgcg
     7501 gcaccgcggg cgtggccggt cgagacatgg tgttcggctt cggcgaccgt gagcccgagc
     7561 agcgccgcga aggctccgaa gaacagcgcc gtgccgtgcg caccgttgag ctggtggcga
     7621 ccgagttcgc gcgtcaccgg cagctgcagc acgtagccgg cgtgctggtc gagggtgctg
     7681 cgcaccacgg cgtggctcgg cagcggcgcg ccgctgtaac cggcgatggt ctcgacgcgg
     7741 cagtcggcga gcagcaccag cgtctgcggg ccgatgcgat cgagctggcg caggctctcg
     7801 gattcctcca gcgcgatcag cacgtcgtac ggcgaaccgc accagccggc gcagcgcacg
     7861 ccgcgcccgg cgcgcagcag gttggtcacc gaggcgcggc ggcgggcgac gccgagctgg
     7921 tcgatcgcgc agacctcgcc gtggctcgtg cgcagcagtt ccgcgagccg cgcgttcagc
     7981 gtcgtcgcgc cgaggccgcc gaggccggca acgcatacgg acagggagct gcgcgggcca
     8041 tgcgcggacg gctggtgcag gaccggtccg gcgggcaggt cggcgtgggg acgctgacgg
     8101 ccggcgtgca gggcttcggt gcgggcggcg ggtgcgtgca tgcgatcaac ctgcgtctgg
     8161 aggagtgaga agataaattt ccggtaattc cggaaaaggc attccggaac aacttgatgg
     8221 aacggaaatc tacggacctg ctggttatcc gtcattacga cgggcatcgt attttggaga
     8281 cgcaagcagt gcttaccacc gcaaattttt agctttttcc ccgtacggcg gatgctgtac
     8341 tcaaatcaat ggattcaagc gcttgcgttt cttgctgtgc gattgcccgg ctgtgagtcc
     8401 ggttttcatt caaacttcgt catctgcgcg tcgggttgca tgactgccga aagtccgccc
     8461 cacgtggggc gacgggcgct gcatcgatgc gccgccggcg gtcccggggc cggttccggc
     8521 ctccgcgacg ccgcgccggt catgcaggcc atgcagatat ccgatgagac ggacaggcgc
     8581 cgtgatctct gcagatgcac gctgcgtctg cagccggcag aatccgcggc cccgggcgcc
     8641 cggcgcccgc agccggtcct gcccgtccat gtctgccgat caccgccgcc gcagcggcct
     8701 tctgctcgtg cttgccgctg ctgccggctt cggactgatg gcggtgttcg cgcgcagcgc
     8761 ctacgccgcc ggcgtgaatc cgccgacgct gctggcgctg cgcttcacgc tggcggcgct
     8821 gctgctggtc gcgatcgtgc aggcgcgcgc ccggccgtgg ccgcgcggcc gcgcgctcat
     8881 cgggctgatc gcgatgggcg ggggcttcta cacgctgatc gcgcagtgct acttcgtggc
     8941 gctgacgcag gcgagtgccg cgctggtggc gctggtgctg tacacgtttc cggtgctggt
     9001 catggcgatc aacgtcgcct gcttcggcga gcgcctgacg gtggcgaagt gcggcgcggc
     9061 gctgctcgcg atggcggggc tcgcgctgac catcggcggc gagctgcacg gcagcgccgg
     9121 cggcatcgcg ctggcgctgg tggccgcagc cggctacgcc gcctacatcc tgatcggtga
     9181 ccgcgtcgcg cgcggcgtcg atccgctctg cggcggcgcg gtcgtgatcg cggccagcgc
     9241 cgtcaccagc gcactgctgg cgctggccga gggcgtgact ctgccgcaga ctggcggcgg
     9301 ctggctggcc gtgaccggca tcgcgctggt ctcgaccgtg ttcgcgatga ccgcgtttct
     9361 cgaagggctg tcgcgcgtcg gctcgaccac tgcggcgatg gcctcgacct tcgagcccgt
     9421 ggtcacggtc gccagcgccg cgtggctgct cggggaaacc gtcagccccg cccggctcgc
     9481 cggcggcggg ctgatcctcg ccgccgtcgt gctgctcgcg cgtgccccgc atcgcccatg
     9541 acgggcagca cgccggctca catccatata aaggagtcac gcatgacgat gcgcccccga
     9601 cgatccgtgc tctacatgcc cggttccaat gcccgcgccc tcgacaaggc gcgcagcctc
     9661 gatgtcgacg cactgatcct cgatctggag gattcggtcg cccccgaggc caagggactc
     9721 ggccgcgagc agatcgccgc cgccctgacc gagggtggct acgggcaccg cgaggtcatc
     9781 gtgcgcgtca acgcgctgtc cacgccctgg ggccacgatg acgtgcgcgc cctcgcaaag
     9841 ctcggcgccg acgccttcct gttcccgaag atcgattcgc cggcgcaggt gatcgaggcc
     9901 gtcaccgcgc tcgatgccgc cggtgccccg tccgggaccg gcgtctggat catggccgag
     9961 acgccgctcg gcatcctcaa catcgccgcg atcgccgcct gctcgccgcg gctgcggtgc
    10021 atcgtcctcg gcacctcgga cctcgccaag gacttgcgcg cccgccacag ccccgggcgc
    10081 atcgccttcg tgacctcgct cggcctctgc gtgctcgccg cccgcgccag cggcctcgac
    10141 gcgctcgacg gcgtgcacct cgacctcgac gacgaggccg gcctcgccgc agtctgcgag
    10201 cagggccgcg acatgggctt cgacggcaag acgctgatcc accccaggca gatcgccgcc
    10261 gccaaccgcg ccttcgcgcc gtccgcggcc gagatcggcg aagcccgcgc catcatccac
    10321 ggctggaccc aggccgctgc cgagggcaag ggcgtctgcc gcgtcaacgg caagctggtc
    10381 gagcacctgc acgtgatcga ggcgacgcgc atcgtcgcgc tcgccgaggc gattgcggcg
    10441 cgtgatgcgc tggc
//
LOCUS       DAOQIE010000119        10398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3231_length_10398_cov_7.521899, whole genome shotgun sequence.
ACCESSION   DAOQIE010000119 DAOQIE010000000
VERSION     DAOQIE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3231_length_10398_cov_7.521899"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="pyk"
                     /locus_tag="PK144_09000"
     CDS             complement(<1..1089)
                     /gene="pyk"
                     /locus_tag="PK144_09000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNF65903.1"
                     /translation="MHRLRNAKIVATLGPASSAPDTIRALFDAGADVFRLNFSHGSHE
                     DHRARLERIRAIERETGRPVGVLADLQGPKLRVGTFAHGPVTLAEGAAFRLDLERDRP
                     GDAGRVAMPHPEIFAALTEGAELLLDDGRLRLRVERCGPGYADTRVIAGGLLSERKGV
                     NVPDVVLPLSALTAKDRADLDFALELGVDWIALSFVQRAADLDELRALVRGRAGLVAK
                     LEKPAAIASLDAIVAASDALMVARGDLGVELPPEQVPAIQKRIVRACRRAGKPVIVAT
                     QMLESMVGAPVPTRAEASDVATAIYDGADAVMLSAESASGRFPLEAVAMMDRIIARTE
                     ADPEGRAASGDGGALPAAQGAATDRAAAI"
     gene            complement(1092..2399)
                     /locus_tag="PK144_09005"
     CDS             complement(1092..2399)
                     /locus_tag="PK144_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNF65904.1"
                     /translation="MSMNMDIRNPRRLLRAMFDAAVASARPEHCIPAHLPAAPAGRTV
                     VIGAGKASAAMAQALERHWPGELSGLVVTRYGYAVPCERIDIVEAAHPVPDAAGEAAA
                     RRLLEQVRGLGADDLVISLISGGGSALLALPGEGLSLADEQAVSRALLACGATISEMN
                     CVRRHLSGIKGGRLAAACHPARVVNLLISDVPGDDPADIASGPTVADVTTCADALAIV
                     RRQRIELPAAAWRRLEDGSGETLKPDDPRLARVSTRMVSTPQQALEAAAAVARAAGVT
                     PFILGDAIEGEARDVGRVLAGITRQVATRGQPFTAPCVLISGGETTVTVRGSGRGGRN
                     VEYLLAQTIALQGLTGVHALAADTDGVDGQEEIAGAFAGPDTLMRARALGIRPDAALA
                     ANDGHGFFGALGDALITGPTLTNVNDFRAILITGDAATPFPEG"
     gene            complement(2412..3767)
                     /locus_tag="PK144_09010"
     CDS             complement(2412..3767)
                     /locus_tag="PK144_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNF65905.1"
                     /translation="MLRHLPGKLLGKLYVQVLIGVSAGILLGVLDPGLGADLKPLGDA
                     FIKLIKMVFAPVIFATVVLGIARMENMKELGRVGARALIYFEVLSTFALLLGLIVVNL
                     VQPGQGMNIDPAALDARAIASYTSAPKSAGFVDFMLNLIPVSVVDALAKNDILQILVF
                     AVFFGVALSHIGPRAKPVVDFLDSFVHGIFAVVGMVMRLAPIAAFGAMAFTVGKYGFG
                     SIASLAKLMGTMYITCLLFVSLVLGGIARLCGFSLWRFLVYIKDEIFTVLATSSSESV
                     VPQLMRKLEHAGVSKPVVGLVVPSGLTFNPDGQCIYYTMAAIFIAQATNTPLTLTDQM
                     VVLGVLLLSSKGSAGVTGSGFITLAATLASLGSIPVAGMVLLLGVDRFMSEARAITNT
                     IGNAVGTLAIARWVGAVDRERLDAVLAGRAEPQNLETLYEEDDAAPAPPARDFPLRRT
                     A"
     gene            complement(3893..4981)
                     /locus_tag="PK144_09015"
     CDS             complement(3893..4981)
                     /locus_tag="PK144_09015"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HNF65906.1"
                     /translation="MKTYKIATIPGDGIGKEVVPAGREVLAAVAAAGQGFRFEFTDYD
                     WGGDYYRTHGVMMPEDGLETLRGQDAILFGSAGDPDIPDHITLWGLRLKICQGFDQYA
                     NVRPTRILPGIDAPLKRCRAEDLDWVIVRENSEGEYSGVGGRVHQGHPIETATDVSVL
                     TRVGCERVLRFAFELARSRPRKQLTVITKSNAQRHAMVMWDQIAAEIAREFPDVRWDK
                     ELVDAATARMVNRPASLDTIVATNLHADILSDLAAALAGSLGIAPTGNIDPQRRYPSM
                     FEPIHGSAFDIMGKGLANPVGTFWSVVMLLEHLGERAAAASVMRAVERVTADAALHTR
                     DLGGTATTADVTRAVCAFLAADAVSRAA"
     gene            5098..6027
                     /locus_tag="PK144_09020"
     CDS             5098..6027
                     /locus_tag="PK144_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF65907.1"
                     /translation="MGAAIQPTDLGFFATLAASPSLSAAARELGISTAAVSRHLAQIE
                     ARLGVLLVNRTSRRMGLTPEGEVFLEHARRILGEIDDLDQLLADARGTPKGLLRVNAT
                     LGFGRLHVAPVTSRYVASHPGVDVQLQLSVSPPPVTDDLYDVCVRFGEPADGRVIARR
                     LAGNRRLLCASPRYLAAHGVPQTPDDLRRCNCITIRQGDDAYGTWRLSDARAPATGEE
                     TVKVRGNLTTNDGEIAVHWALDGHGILLRAEWDIARYLQSGRLVQVLPDYRTPDADIY
                     CVYPRRHQLSQRVRTFVDFLAHAFATAGAAQPR"
     gene            complement(6035..6184)
                     /locus_tag="PK144_09025"
     CDS             complement(6035..6184)
                     /locus_tag="PK144_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65908.1"
                     /translation="MYLQGRRLIRYSRGHVTVLDRSGLEAASCPRYEADRRIYARIMD
                     SMTRA"
     gene            complement(6541..7572)
                     /locus_tag="PK144_09030"
     CDS             complement(6541..7572)
                     /locus_tag="PK144_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNF65909.1"
                     /translation="MPRRPAIKPLRTPKTRTLAGPASHIGTGLRRLLSGIATPAEAPI
                     VHARAAGRWEEGSWGLGPLAQRRFRLYLPPGATRARPVPVLLLLHGCGQDSASFAAST
                     RVARLAQREQFAVLMPEQTQQANAQRCWNWFRSEAQVATECLLLLSIVEHACLRWPLR
                     RDRLYALGLSAGGAMALTLGLRHPERFVAVGSHSGAVPASASNPLQAAAAMQGRRLPD
                     VKQLRLRLFGRRPPPMIVLHGDADRIVAPACAQASAALWLELLPAGTALKSRGRELQR
                     GERLGMHVTDWQHADGDPYVRAVRVHELGHAWSGGAADQAFSEPAGPDALTLAWRFFD
                     AATEARGRR"
     gene            7985..9271
                     /locus_tag="PK144_09035"
     CDS             7985..9271
                     /locus_tag="PK144_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF65910.1"
                     /translation="MPDALIYAHAPSYDAGHHRWIMGAFTMQALRFNFRTLCRNAAER
                     GEQLQARLLDALGARHGAKALPDFGIERAAQLIGCTANYIRSLEARAEAPQPRLIEAG
                     SLQRRVYNYNDVNHLRTVMGRGVHRPDGAAAARVVFSNLKGGVAKTTMSLHFAQYCAR
                     EGYRVLLVDADPQATTTGAFGFIPDLDLGDGDDLSQALLAGPQHLETAIRRTHWAQLD
                     LLPSRLALQYVDWQLSQPAERTHAELGAPPVRLTRALAQVEDRYDLIVIDTPPSLGML
                     SINAIAAASLIVMPIVPHMYDISSSVQYFRILEQVCELYAREIDVARLAILLTKVDNS
                     TETLNNIAVINGAYGELILSNRMGLTKELQKSASDQLSIYEIDQPRGSRDTYHRAVMM
                     LDAVNSELLLAVRQLWQQQVLVGIEAAEAGTGRVRA"
     gene            9268..10158
                     /locus_tag="PK144_09040"
     CDS             9268..10158
                     /locus_tag="PK144_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF65911.1"
                     /translation="MSRRGSGGLKGLAGFLGSGEHQFGQSLMDESAAGVARLPVAQLV
                     PGRFQPRRTIDEAWLAELADSVRRLGVIEPVAVRPLGEGRYEILAGEMRWRAAQQAGL
                     VELPVIVHDVDDRTAASMALVENLLRRDLDPIEEAHALARLGSEFGLRNRELAELLSR
                     SESAISRTLGLLALAPGVQALLQARRLDAGHARVLLGLPEADQEALAQAAVDLGLSVR
                     ELEKRRQRLLAGERPALAEPVVPADAELDALERQFTQRLGAGVRLRYSAARQRGRIEI
                     DFRDLDGLRALLARLPEAGG"
BASE COUNT         1518 a         3599 c         3802 g         1479 t
ORIGIN      
        1 gatggccgcg gcgcggtcgg tcgccgcgcc ctgcgccgcg ggcagggcac cgccgtcgcc
       61 gctcgcggcc cggccctcgg ggtcggcctc ggtgcgcgcg atgatgcggt ccatcatcgc
      121 caccgcctcc agcgggaagc ggccggaggc cgactcggct gacagcatca ccgcgtcggc
      181 accgtcgtag atggcggtgg cgacgtccga ggcctcggcg cgggtcggca ccggcgcgcc
      241 gaccatggat tcgagcatct gcgtggcgac gatcactggc ttgccggcgc ggcggcaggc
      301 ccgcacgatg cgcttctgga tcgccggcac ctgctcgggc ggcagctcca cgccgagatc
      361 gccgcgcgcg accatcagcg cgtcgctggc ggcgacgatc gcatcgaggc tggcgatcgc
      421 ggccggtttt tccagcttgg cgacgaggcc ggcacggccg cggaccagcg cgcgcagctc
      481 gtcgaggtcc gcggcccgct gcacgaacga cagcgcgatc cagtcgacgc cgagttcgag
      541 cgcgaagtcg agatcggcgc ggtccttggc ggtcagcgct gacagcggca gcaccacgtc
      601 cggcacgttg acgcccttgc gctccgacag caggccgccg gcgatgacgc gggtatcggc
      661 gtagccgggg ccgcagcgtt cgacgcgcag acgcaggcgg ccgtcatcga gcagcagctc
      721 ggcgccctcg gtcagtgcgg cgaagatttc cgggtgtggc atcgccaccc ggccggcatc
      781 gccggggcgg tcgcgctcca ggtcgagccg gaacgccgcc ccttcggcga gcgtcaccgg
      841 gccgtgcgcg aaggtgccga cgcgcagctt cggtccctgc aggtcggcaa gcacgccgac
      901 cggccggccg gtctcgcgct cgatggcgcg gatccgttcc agccgcgcgc ggtgatcctc
      961 gtggctgccg tggctgaagt tgaggcggaa cacatcggcg ccggcatcga acagcgcgcg
     1021 gatggtatcg ggggcggagc tcgccgggcc gagggtggcg acgatcttgg cattgcgcag
     1081 tcggtgcatg gtcagccttc cggaaacggt gtggccgcat cacccgtgat caggatggcg
     1141 cggaagtcat tgacgttggt gagggtcggg ccggtgatca gcgcatcgcc gagcgcaccg
     1201 aagaagccgt ggccgtcatt ggccgcgagg gcggcatccg ggcggatgcc gagggcgcgg
     1261 gcgcgcatca gcgtgtcggg gccggcgaag gcgccggcaa tctcctcctg gccgtcgacg
     1321 ccgtcggtat cggcggcgag cgcatgcacg ccggtcaggc cctgtagcgc gatcgtctgc
     1381 gccagcaggt actcgacgtt gcgcccgccg cgaccgctgc cgcgcacggt gacggtggtc
     1441 tcgccgccgg agatcagcac acacggggcg gtgaacggct ggccgcgcgt ggcgacctgg
     1501 cgggtgatgc cggcgagcac cctgcccacg tcgcgcgcct cgccctcgat cgcatcgccg
     1561 aggatgaagg gcgtgacgcc ggcggcacgc gccaccgctg ctgcggcttc cagcgcctgc
     1621 tgcggcgtcg acaccatccg ggtgctgacc cgcgccagcc gcggatcgtc gggcttcagc
     1681 gtctcgccgc tgccgtcttc gagccggcgc caggccgcgg ccggcagttc gatgcgctgg
     1741 cgccgcacga tcgccagcgc atcggcgcag gtcgtgacat cggccacggt cgggccggag
     1801 gcgatgtcgg cggggtcatc gccgggcacg tccgagatca gcaggttcac cacgcgcgcc
     1861 ggatggcagg ctgccgccag ccggccgccc ttgatgccgg agaggtggcg gcgcacgcag
     1921 ttcatctccg agatcgtcgc gccgcaggcc agcagcgcgc ggctgacggc ctgttcgtcc
     1981 gcgaggctca ggccttcgcc cggcagcgcc agcagcgccg agccgccgcc ggagatcagg
     2041 ctgatgacga gatcatcggc gcccagcccc cggacctgct ccagcagccg gcgggcggcg
     2101 gcctcgccgg cggcatcggg caccgggtgg gcggcctcga cgatgtcgat gcgctcgcac
     2161 ggcaccgcgt agccgtagcg ggtcacaacc aggccgctca gttcgcccgg ccagtgccgt
     2221 tcgagcgcct gtgccatcgc ggccgaggcc ttgccggcac cgatcaccac ggtccggccg
     2281 gcgggcgctg cgggcagatg ggcggggatg cagtgttcgg gtcgggcact ggcgacggcg
     2341 gcatcgaaca tcgcgcgcag cagccggcgg ggattgcgga tatccatgtt catgctcatg
     2401 tcctccgccg ctcaggccgt gcgccgcagc ggaaagtcgc gggccggcgg cgcaggcgct
     2461 gcgtcgtcct cctcgtacag cgtttccagg ttctgcggtt cggcgcggcc ggccagcacg
     2521 gcgtccagcc gttcgcggtc gacggcgccg acccagcggg cgatggccag cgtgccgacg
     2581 gcgttgccga tggtgttggt gatggcgcgg gcttcggaca tgaagcgatc gacgccgagc
     2641 agcagcacca tgccggcgac cgggatgctg ccgagcgagg caagcgttgc ggcgagcgtg
     2701 atgaagccgg agccggtgac gccggccgaa cccttggagc tcagcagcag cacgccgagc
     2761 acgaccatct ggtcggtcag cgtcagcggc gtgttggtgg cctgggcgat gaagatcgcg
     2821 gccatcgtgt agtagatgca ctgcccgtcc gggttgaagg tcaggcccga gggcacgacc
     2881 agcccgacca ccggcttcga gacgccggca tgttcgagct tgcgcatcag ctgcggcacg
     2941 accgattcgg acgaactggt cgcgagcacc gtgaagatct cgtccttgat gtagacgagg
     3001 aagcgccaga ggctgaagcc gcagagccgg gcgatgccgc cgagcacgag gctcacgaac
     3061 agcaggcagg tgatgtacat cgtgcccatc agcttcgcga gcgaggcgat cgagccgaag
     3121 ccgtacttgc cgaccgtgaa tgccatcgcg ccgaaggccg cgatcggggc aagccgcatc
     3181 accatgccga cgaccgcgaa gatgccgtgc acgaaggaat cgaggaaatc cacgaccggc
     3241 ttcgcacgcg gcccgatgtg cgacagcgcc acgccgaaga acaccgcgaa caccaggatc
     3301 tgcaggatgt cgttcttcgc cagcgcatcg accacgctga ccgggatcag gttgagcatg
     3361 aagtcgacga agccggcgga cttcggcgca ctcgtgtagc tggcgatcgc cctggcatcg
     3421 agggcggccg gatcgatgtt catgccctgg ccgggctgga ccaggttgac gacgatcagc
     3481 ccgagcagca gcgcgaaggt cgacagcacc tcgaagtaga tcagcgcgcg cgcaccgacg
     3541 cggccgagct ccttcatgtt ctccatgcgc gcgatgccga gcacgacggt ggcgaagatc
     3601 accggcgcga acaccatctt gatcagcttg atgaaggcgt caccgagcgg cttgagatcg
     3661 gcaccgaggc cgggatcgag cacgccgagc aggatgccgg cggatacgcc gatcagtacc
     3721 tgcacgtaga gtttgccgag gagcttgcca ggcagatgtc gaagcatcgg agttctcctc
     3781 ccgtggtgga cgggacagac gaggaccctg ccgctcttgc tggcggcatc gcggatcggg
     3841 aacggcgggg aggcggtccg gcacgcagca ggcgcagccg gaccgccgcg gctcaggcgg
     3901 cgcggctgac ggcgtccgcg gcgaggaagg cgcacaccgc gcgggtgacg tcggccgtgg
     3961 tggccgtgcc gcccagatcg cgggtgtgca gggccgcatc ggcggtgacg cgctcgaccg
     4021 cgcgcatcac gctggccgcc gcggcgcgct cgcccaggtg ttcgagcagc atcacgacgg
     4081 accagaaggt gccgaccgga ttggccaggc ccttgcccat gatgtcgaag gccgaaccgt
     4141 gtatcggctc gaacatcgac gggtagcggc gctgcggatc gatgttgccg gtcggcgcga
     4201 tgccgaggct gccggcgagg gcggcggcca gatcgctcag gatgtcggcg tgcaggttgg
     4261 tcgcgacgat ggtgtcgagc gaggccgggc ggttgaccat gcgcgcggtc gcggcatcga
     4321 ccagttcctt gtcccagcgc acgtccggga actcgcgggc gatctcggcg gcgatctggt
     4381 cccacatcac catcgcgtga cgctgggcgt tgctcttggt gatcaccgtc agctgcttgc
     4441 gcggccgcga gcgggccagt tcgaaggcga agcgcagcac gcgctcgcag ccgacgcggg
     4501 tcagcaccga cacgtcggtg gccgtctcga tcggatggcc ctgatgcacg cgcccgccga
     4561 cgcccgagta ctcgccttcg gagttctcgc gcacgatcac ccagtccaga tcctcggcgc
     4621 ggcagcgctt cagcggtgcg tcgatgcccg gcaggatgcg ggtcggacgc acgttcgcgt
     4681 actggtcgaa gccctggcag atcttcaggc gcaggcccca gagcgtgatg tggtcgggga
     4741 tgtccgggtc gccggccgag ccgaacagga tcgcgtcctg gccgcgcagc gtctccaggc
     4801 cgtcctccgg catcatcacg ccgtgcgtac ggtagtagtc gccaccccag tcgtaatcgg
     4861 tgaactcgaa gcggaaaccc tggccggcag cggcgacggc ggcgagtacc tcgcggcccg
     4921 cgggcacgac ctccttgccg atgccgtcgc cggggatggt ggcgattttg taggtcttca
     4981 tgggggttct cctctctcgg ggtggtgtgg acgagcggac tgtacggact cgtccttctc
     5041 ccggagcggg gctaggctca aaccgtcatt aacctgaagt taaaggcggg cccgctcatg
     5101 ggagctgcaa tccagccgac cgatctcggt ttcttcgcca cgctcgcggc cagccccagc
     5161 ctgagcgcgg cggcgcgcga gctcggcatc agcacggcgg cggtcagccg gcatctggcg
     5221 cagatcgagg cgcgcctcgg cgtgctgctg gtcaaccgca ccagccggcg catggggctc
     5281 acgcccgagg gcgaggtgtt cctggagcac gcgcggcgca tcctcggcga gatcgacgat
     5341 ctggatcagc tgctcgccga tgcgcgcggc acgccgaagg ggctgctgcg tgtcaacgcc
     5401 acgctcggct tcggccggct gcacgtcgcc ccggtgacct cgcgctacgt cgcgagccac
     5461 cccggcgtcg acgtgcagct gcagctgtcg gtcagtccgc cgccggtcac cgacgatctc
     5521 tacgacgtct gcgtgcgctt cggcgaaccg gccgacggtc gcgtgatcgc gcggcggctg
     5581 gcgggcaacc gccgcctgct ctgcgcctcg ccgcgctacc tcgcggcgca cggcgtgccg
     5641 cagaccccgg acgacctgcg ccgctgcaac tgcatcacga tccgccaggg cgacgacgcc
     5701 tacggcacct ggcgcctcag tgacgcccgc gccccggcca ccggcgagga aacggtgaag
     5761 gtgcgcggca acctgaccac caacgacggc gagatcgcgg tgcactgggc gctcgacggt
     5821 cacggcatcc tgctgcgcgc cgaatgggac atcgcccgct acctgcagag cggccggctg
     5881 gtgcaggtgc tgcccgacta ccgcacgccg gatgccgaca tctactgcgt gtatccgcgc
     5941 cggcaccagc tctcgcagcg cgtgcgcacc ttcgtcgact tcctcgcgca cgccttcgcc
     6001 acagccggcg ccgcgcagcc ccgctgagcc ccggttacgc gcgggtcatg gaatccatga
     6061 tccgcgcgta tatgcgccgg tcggcctcgt agcgcgggca cgaagcggct tcgagaccgc
     6121 tgcggtcgag caccgtgacg tggccgcggc tgtagcggat cagccggcgg ccctgcaggt
     6181 acacgactga taccgatcac gcgtaaatgc gttaatgcgt tgttttcccc tctcccgcga
     6241 ggggcgaggg gctgaaaaga cggcgtgttc agctgtgatg ggtatgactg gcggtctcgc
     6301 ggccggacgg gccgctccgg cggcgggcgg cctggtcaga acgtggcggg agctgcggga
     6361 aggcggcgtc ggtccggtga cggcccggac gggggcggac gcaggtctgc cgcgggcgtg
     6421 caggccgttg tccgggatcg atgggctgcg atggtgccgc cgggccgggt ggcgcctgcc
     6481 ggcgaggccg gtcgtccggc gcgctggcgg cgccggtttc cacggctgtc cgggcgcgcc
     6541 tcagcgccgt ccgcgcgcct cggtcgccgc gtcgaagaaa cgccaggcca gcgtcagcgc
     6601 gtccggcccg gccggttccg agaaggcctg gtcggcggcg ccgccgctcc aggcgtggcc
     6661 gagttcgtgg acgcgcacgg cgcgcacgta gggatcgccg tcggcgtgct gccagtcggt
     6721 gacgtgcatg ccgaggcgct cgccgcgctg cagttcgcgg ccgcggcttt tcagcgccgt
     6781 gccggccggc agcagctcca gccacagcgc cgccgaggcc tgcgcgcagg ccggtgcgac
     6841 gatgcgatcg gcgtcgccgt gcagcacgat catcggcggc ggccggcgtc cgaacagacg
     6901 caggcgcagc tgtttcacgt ccggcaggcg ccggccctgc atcgcggcag cggcctgcag
     6961 cgggttgctg gcgctcgccg gcaccgcgcc cgaatgcgag ccgaccgcga cgaagcgttc
     7021 cgggtgacgc agaccgagcg tgagcgccat cgcgccgccg gccgacagcc cgagcgcgta
     7081 cagccggtcg cggcgcagcg gccagcgcag gcaggcgtgt tcgacgatcg acagcagcag
     7141 caggcattcg gtcgcgacct gtgcctcgct gcgaaaccag ttccagcagc gctgggcgtt
     7201 ggcctgctgc gtctgctcgg gcatcagcac cgcgaactgc tcgcgctgcg cgaggcgcgc
     7261 aacgcgcgtg ctggcggcga agctggcgct gtcctgcccg cagccgtgca gcagcagcag
     7321 gacgggcacc ggccgggcgc gcgtcgctcc cggcggcagg tacagccgga aacgccgctg
     7381 cgccagcggc ccgaggcccc agctgccttc ctcccagcgc ccggcggcgc gggcgtggac
     7441 gatcggcgcc tcggccggtg tggcgatgcc ggacagcagc cggcgcaggc cggtaccgat
     7501 atggctggcg ggcccggcga gcgtgcgggt tttcggcgta cgcaggggtt tgatggcggg
     7561 acgacgcggc ataagggcga cggattcggc gatcgggggg tgtgggcagc ctacccgagc
     7621 cgccgggggc agcgggcgat cggcgtttgc ggatcgtgga gacttgccac gtggcaagcc
     7681 gcgacggatc gggcggaccg gcagaacggg ttctggcgtg gtgcaagcgg aggcttgcac
     7741 cgggctgcgc cagatcagcc ctcgctcgcc ggcccgccgc ttgcaggcat gcgacctgtg
     7801 tcggaaaccc gtgtcgctcg tatcagaatt acgtcaagtc gttgtattga tgcgactgtt
     7861 ttccacagat tctgtggaca aagacggcag ttctgtgaca ggcgtcaaaa gcctgtcatg
     7921 ccgcagcgcg acatatgcct gcgatcggtg atccgggcgt gatccggact cgtgcgtatc
     7981 attcatgcct gatgcgttga tttatgcgca cgcgccttcc tacgatgccg gccatcaccg
     8041 atggatcatg ggggcattca cgatgcaggc gctgcgcttc aacttccgca cgctgtgccg
     8101 aaacgccgcc gaacgcggcg agcagttgca ggcgcggctg ctcgatgcgc tcggggcgcg
     8161 ccacggtgcc aaggcgctgc cggatttcgg catcgagcgc gccgcgcagc tgatcggctg
     8221 cacggccaac tacatccgca gcctggaggc acgcgccgag gcaccgcagc cgcggctgat
     8281 cgaggccggc agcctgcagc ggcgcgtata caactacaac gacgtcaacc acctgcgcac
     8341 ggtcatgggc cgcggcgtgc accggccgga cggagccgcg gcggcgcgcg tggtgttctc
     8401 gaacctcaag ggcggcgtcg ccaagaccac gatgtcgctg cacttcgcgc agtactgcgc
     8461 gcgcgagggc taccgcgtgc tgctggtcga tgccgatccg caggcgacca ccaccggcgc
     8521 cttcggcttc atcccggatc tcgatctcgg tgacggcgat gacctgtcgc aggcgctgct
     8581 cgccggcccg cagcacctcg aaaccgcgat ccggcgcacg cactgggcgc agctcgacct
     8641 gctgccctcg cgcctggcgc tgcagtacgt cgactggcag ctgtcgcagc cggccgaacg
     8701 cacccatgcc gaactcgggg cgccgccggt gcgcctgacg cgcgcgctcg cgcaggtcga
     8761 ggatcgctac gacctgatcg tcatcgacac gccgccgtcg ctcggcatgc tgtcgatcaa
     8821 cgccatcgcc gcggccagcc tgatcgtgat gccgatcgtg ccgcacatgt acgacatctc
     8881 ctcgtcggtg cagtacttcc gcatcctcga acaggtctgc gagctgtacg cgcgcgagat
     8941 cgacgtggcg cggctggcga tcctgctgac caaggtcgac aacagcaccg agacgctgaa
     9001 caacatcgcc gtgatcaacg gcgcctacgg cgagctgatc ctcagcaacc gcatgggcct
     9061 gaccaaggag ctgcagaagt cggcgtccga tcagctcagc atctacgaga tcgaccagcc
     9121 gcgcggctcg cgcgacacct accaccgcgc ggtgatgatg ctcgatgccg tcaacagcga
     9181 gttgctgctc gcggtgcgcc agctctggca gcagcaggtg ctggtcggca tcgaggcggc
     9241 cgaggccggg accggccggg tgcgcgcatg agccggcgcg gcagcggcgg tctgaagggc
     9301 ctggccggtt ttctcggcag cggcgagcac cagttcgggc agagcctgat ggacgagtcc
     9361 gccgccggtg ttgcgcgcct gccggtcgcg cagctcgtgc ccggacgctt ccagccgcgg
     9421 cgcacgatcg acgaagcctg gctcgccgaa ctggccgaca gcgtgcgccg gctcggtgtc
     9481 atcgagccgg tcgccgtgcg cccgctcggc gaaggccgct acgagatcct cgccggcgag
     9541 atgcgctggc gtgccgcgca gcaggcgggg ctcgtcgagc tgccggtcat cgtccacgat
     9601 gtcgatgacc gcacggcggc gtcgatggcg ctggtcgaga acctgctgcg ccgcgacctc
     9661 gatccgatcg aggaggcgca cgcgctcgcg cgcctcggca gcgagttcgg cctgcgcaac
     9721 cgcgaactcg ccgaactgct gagccgctcc gaatccgcca tcagccgcac gctcggcctg
     9781 ctcgcgctcg cccccggcgt gcaggcgctg ctgcaggcgc gccgcctcga tgccggtcac
     9841 gcccgcgtgc tgctcgggct gccggaggcg gatcaggagg cgctggcgca ggctgcggtc
     9901 gatctcggcc tgagcgtgcg cgagctggaa aaacgccggc agcggctgct cgccggcgaa
     9961 cggccggcgc tcgccgagcc ggtcgtgcct gccgatgccg aactcgatgc gctcgaacgc
    10021 cagttcacgc agcgcctcgg cgccggcgtg cgcctgcgct acagcgcggc aaggcagcgc
    10081 ggccgcatcg agatcgactt ccgcgacctc gacggcctgc gtgcgctgct ggcgcggctg
    10141 ccggaggcgg gcggctgaag ccggaacttg ccacgtggca agtcgaaggt ggcaacgcgc
    10201 atccgccggc ggtgacggct ggcgtcccgg ctcacgctga acgtcgggcc gggcgcagcc
    10261 ggaacttgcc acgtggcaag tcgaaggcgg cagcgcgcat ccgccggcgg tgacggctgg
    10321 cgtcccggtt cgccgtgaac gccgggaccg gatgcagccg caacttgcca cgtggcaagt
    10381 ccggtgccct cctgatca
//
LOCUS       DAOQIE010000120        10346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3254_length_10346_cov_6.463317, whole genome shotgun sequence.
ACCESSION   DAOQIE010000120 DAOQIE010000000
VERSION     DAOQIE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_10346_cov_6.463317"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK144_09045"
     CDS             complement(<1..383)
                     /locus_tag="PK144_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF65912.1"
                     /translation="MTATTAGHDAVHDGHDHGHEHAPPRGLWRWLSTTNHKDIGTMYL
                     VLSLTMFFIGGAMALVIRAELFRPGLQLVEPMFFNQMTTMHALVMIFGAVMPAFVGLA
                     NWLIPMMVGAPDMALPRMNNWSFWI"
     gene            complement(398..1582)
                     /gene="coxB"
                     /locus_tag="PK144_09050"
     CDS             complement(398..1582)
                     /gene="coxB"
                     /locus_tag="PK144_09050"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009833313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNF65913.1"
                     /translation="MSAFRPCGLAATGVLGLWSAAVHADFQLNMTPGVTAVSHRIYEL
                     HMLIFWICVAIGIVVFGVMFWSVYHHRKSRGAVAAQFHESTTIEIAWTIVPVLILVGM
                     AIPATKTLVQMEDTSNADLTIKVTGYQWKWRYDYLGEDIGFYSSLKTDARQIHGETDK
                     GEHYLLEVDRPLVVPVGRKVRILTTASDVIHAWWVPALGWKRDAIPGFVNESWAKIDQ
                     PGTYRGQCAELCGKDHGFMPIVVVAMNQPDYDKWLADTKAAAASERNSADRVWGKAEL
                     MAKGAEVYRSCAACHQASGEGIAGMFPPLIHGKPFSGPEALLEPLRTRGFLSADGKIT
                     VGPLDKHAEIVLKGIPGTPMAGYAGQLSDVEVAAVLTYIRNSWGNDFGDAVQPSMIKT
                     LR"
     gene            complement(1712..2200)
                     /locus_tag="PK144_09055"
     CDS             complement(1712..2200)
                     /locus_tag="PK144_09055"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNF65914.1"
                     /translation="MVDVECHDEDHEVCFELRPNRSLSATGALLFFAATGCVCVTIAF
                     VCLYFGFWPVVPFTGLELAALGAALWWTQRDNRRRESIRIGAQQVIVERSGRRPMRVA
                     LTRCWVRVSLEPDASGRGSRLELGTHGRPVEIGGFLTEAERQALAGSLRARLRCGRHG
                     YP"
     gene            complement(2570..4750)
                     /gene="pbpC"
                     /locus_tag="PK144_09060"
     CDS             complement(2570..4750)
                     /gene="pbpC"
                     /locus_tag="PK144_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNF65915.1"
                     /translation="MRRNPVWRGAAHTRLRFIPCPGAGQGRGRGRQTTDRPVAGPWSW
                     RARIGVLLLAAVFFALVTADRLLPPELSRLQHESLRVLAADGTLLHVERSADGYWRLP
                     VRLAEVDPAYVAALLAFEDQRFRRHPGVDPLALARAAGQWLRHGRIVSGGSTITMQLA
                     RLLEPRPRTLGAKLIECLRALQLEAHFSKDELLAMYLTLAPYGGNLEGLRAASLAWFG
                     KSPRELRPAEYALLVALPQSPTRLRPDRRPEAARLARDKVLMRLAAAGVLDAARAAEA
                     REEAVPAQRLTLPRAVPHLAAALHAAQPQAFELHSTLDARVQAETATLAQRALAGLGA
                     QVNAALVVVEQRSGRVLAYVGAAELRDPQRHGSVDLLRALRSPGSTLKPLVYGLAFDD
                     GVLHPDTRIDDVPTRFGDYSPGNFMERYYGEVSVREALQRSLNVPAVAVLDRVGPLRV
                     AQRLREAGLELQLPDGAAPGLPIVLGGAGMRLWDLAGLYAALANGGSAPPLTLMEAVA
                     AVPRPPMRLLGPVAAEQLARILEDAPPPAEAVPPGDTRQPRRIAYKTGTSYGFRDAWA
                     LGYDGAHTVGVWVGRPDGSPNPGHFGRNTAAPLLWRVFDLLAPSPRVAFVEQTQLPDG
                     GRALAPNWRHLAGSAARDTRATLALSFPLPGARVALPADGAPLPLVASGGLRPLRWLV
                     NGVPLTGSPMRRSVAWQPDGEGAARVTVVDAAGQSASAEFWLMR"
     gene            complement(4747..9972)
                     /locus_tag="PK144_09065"
     CDS             complement(4747..9972)
                     /locus_tag="PK144_09065"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNF65916.1"
                     /translation="MRALPLLCLLFAWLSAMPAHAFEDKTLGVAADQYRAALIAQAGK
                     VLPPDTQAKADAALKAKKWGEAIVPLELLVRAHPDDGPLWLKLARARGNSNPQDERAR
                     PAAYAALRLAKSAEEQAAALFVLGRLTEVNQDGRAALAMYDEGLALVPDEGIARRAAN
                     LRATLIIKPVNAAVEEESATPRFCVEFSDPLSKARQYHYEDFLQTEPAVRLSVTVRDT
                     KLCAEGVEWGQGYRVTVRAGVPGAKGDRTASSETFTLAFPDRKPSVAFRGNGYVLPRK
                     AGQMLPVVSVNLPAAELKLLRINDRNLIREVLNGSFDEALYGSDEDAIASDRGEEVWH
                     GSVEFAGDKNRETVTLLPLKTVLPETKPGVYVLTARPPRKQAPAAGDEDDDYDYDVHA
                     TQWLIVSDLGLSVLRGADGLHVFVRSLESGRPAPNVELRLLARNNTELATVTSDAEGR
                     AKFDPGLLRASGGRAVRGLYAYGPGGDFNLLDLGQPAFDLSDRGVDGRVQPVGLDAFL
                     YTERGVYRPGEAVELSALLRDAGASAVENLPVTLIVRRPDGEEAERRVLAPGAPGAYH
                     TRLELPANARSGTWTARLHAEPDGPAIGERRFQVEDFVPQQIKVELATDQPFLTPDSA
                     VVLAISGQFLYGAPAAGLPVEMDATLEADPDPYPQFAGYRFGLVQDEWEPAYQNLEGL
                     PQATDEEGRAVARIEAPPVPDTGRPLRLSVRVGIEEPGGRPVRRVVRLPYRSTPYALG
                     IRPGFDAGQGLKQGAAAPFELIAVGADGKVREAKGLRWELYRENWDYYWFHNGDTGRF
                     DYKLLLRDGAPVAGGALDIGMDAPAKLNTAALDYGPYRLEVYDPAGTTASSVRFTVGW
                     YAAPSAGDRPDQLRVTLDQPRYAAGASAKVRVQPPFAGEVLLTVVSHKLWLAHSQYVP
                     AEGATIDLPVDADWGPGVYVSATAFRAGREAPAVGPGRAIGLAWLALDDAPRRLDVKL
                     DAPAEWRPRQTVQLPVTLSGADGSSGGKGVTLTVAAVDEGILQLTDFETPDPVAWYLG
                     KRRLGLDMLDVYGRLIAPAEGRAGELRSGGDADSRLLDGAQLKHRQSVALFSGFVTPD
                     AQGRVSVPLALPDFNGRLRLMAVAWDRTRVGRGEAQVPVRDPLVAEAYLPRFLAPGDR
                     AQVRTQFDDLAAVPGDWQVQYAVSGPVTIEGSPAQTLALGNGRPKQVRGALALSATGV
                     GDARLTLTVTGPDGSRIEREWPFTVRAAQAVQTFAETKALAPGAKLALGADLLGGWLP
                     GSGRVDVLASSRPPIDVPALLDALDRYPYGCAEQTTSRALPLLYANDLARDWLGGEAV
                     DPALPKRVQDAIARLLAQQRSDGGFGLWRALDDPNPWLNAYVMNFLVRAKEQGYLVPE
                     VALKHGLAALHDALNSRSDPDERELATRAASAYVLARAQAATPGELRYLHDAWLAKMP
                     TELAAAQLGAALARYGDSERARAAFDHAFALQARPYYWWDYGSALRDRAALLALATEA
                     GQSARIGDLATDVAREADGRRYLSTQEQAWLLLAAQALGAGQGQLAISRDGAPWAQGK
                     ARARLRPEAAALERGLTLANDAQAQAWVRVTRSGIPSADQPAASEGITVTRQWYTLDG
                     KPLPPGPGPVKAGTTLVAVIGGEVAAGEYVQGLVVDLLPAGFELENAALGPNRAPSEL
                     PWLPTLTEPDHVELRDDRYVAAIGLEDGTRRFTLAYLVRAITPGDYRVPAVLAEDMYR
                     PQLRGRGAMGRVKIE"
     gene            10166..>10346
                     /locus_tag="PK144_09070"
     CDS             10166..>10346
                     /locus_tag="PK144_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNF65917.1"
                     /translation="MSTVYWREVDRVYIAEVPELPGCMADGASQEEAVANARQVIEYW
                     IATAKELGHAAPEPRG"
BASE COUNT         1508 a         3717 c         3676 g         1445 t
ORIGIN      
        1 atgatccaga acgaccagtt gttcatgcgc ggcagcgcca tgtccggcgc gccgaccatc
       61 atcgggatca gccagttggc aagcccgacg aaggccggca tcaccgcgcc gaagatcatg
      121 accagcgcgt gcatcgtggt catctggttg aagaacatcg gctcgaccag ctgcaggccg
      181 ggccggaaca gctcggcgcg gatcaccagc gccatcgccc cgccgatgaa gaacatcgtc
      241 agcgacagca cgaggtacat cgtgccgatg tccttgtggt tggtcgtgct cagccagcgc
      301 cacagcccgc gcggcggcgc gtgctcgtgg ccgtggtcgt gtccgtcgtg aacggcatcg
      361 tgtccggcgg tggtcgcggt catgtccggt ctcccgttca gcgcagggtc ttgatcatcg
      421 agggctggac cgcgtcgccg aagtcattgc cccaggaatt gcggatgtag gtcagcacgg
      481 cggcgacctc gacgtccgag agctggccgg cgtagcccgc catcggcgtg ccggggatgc
      541 ccttcagcac gatctcggcg tgcttgtcga gcggcccgac cgtgatcttg ccgtcggccg
      601 acaggaagcc gcgcgtgcgc agcggttcga gcagcgcctc ggggccggag aacggcttgc
      661 cgtggatcag cggcgggaac atgccggcga tgccttcgcc actggcctga tggcaggccg
      721 cgcagctgcg gtagacctcg gcacccttgg ccatcagctc ggccttgccc cagacgcgat
      781 cggccgagtt gcgctcggag gcagcggcag ccttggtgtc ggcgagccat ttgtcgtaat
      841 caggctggtt catcgcgacg acgacgatcg gcatgaagcc gtgatccttg ccgcagagct
      901 cggcgcactg gccgcggtag gtgccgggct ggtcgatctt cgcccaggac tcgttgacga
      961 agcccgggat cgcatcgcgc ttccagccga gcgccggcac ccaccaggcg tggatcacgt
     1021 ccgaggccgt ggtcaggatg cgcaccttcc tgccgaccgg cacgaccagc ggcctgtcga
     1081 cttcgagcag gtagtgctcg cccttgtcgg tctcgccgtg gatctggcgg gcgtcggtct
     1141 tcaggctcga atagaagccg atgtcctcgc cgaggtagtc gtagcgccac ttccactggt
     1201 agccggtgac cttgatcgtc aggtcggcgt tggaggtgtc ttccatctgc accagcgtct
     1261 tcgtggccgg gatcgccatg ccgaccagga tcagcaccgg cacgatcgtc caggcgatct
     1321 cgatcgtcgt gctctcgtgg aactgcgccg ccactgcgcc gcgcgacttg cggtggtggt
     1381 acacggacca gaacatcacg ccgaacacga cgatgccgat cgccacgcag atccagaaga
     1441 tcagcatgtg cagctcgtag atgcggtggc tgacggcggt gacgccgggc gtcatgttga
     1501 gctggaagtc ggcgtgtacc gccgcgctcc acagcccgag gacgccggtc gcggcgaggc
     1561 cgcagggacg gaaggctgac atgcccgatg tactcctcag gtggcgaccc gtacgtcgcg
     1621 ggtccggagc gggtgtgtgc agggcacccg ccacgatggt tgcaagcctt cggtcggcga
     1681 ccgccggaaa gcgccggggc cgtctcctta tttatgggta tccgtgcctg ccgcagcgca
     1741 gccgcgcgcg cagcgagccg gccagcgcct gtcgctcggc ttcggtcagg aagccgccga
     1801 tctcgaccgg ccggccgtgc gtgccgagtt cgagccggct gccgcgcccg ctcgcgtccg
     1861 gctccagcga cacgcgcacc cagcagcggg tcagcgccac gcgcatcggt cgccggccgc
     1921 tgcgctcgac gatcacctgc tgcgctccga tgcggatcga ctcgcgccgg cggttgtcgc
     1981 gctgcgtcca ccacagcgcc gcaccgagcg ccgccagctc caggccggtg aacggcacga
     2041 ccggccagaa gccgaagtaa aggcagacaa aggcgatcgt cacgcagacg cagccggtcg
     2101 ctgcgaagaa cagcagcgcg ccggtggcgg acagggaacg gttcggccgc agctcgaagc
     2161 agacctcatg atcttcgtca tggcattcaa cgtcgaccat gaccctcgct ccggcatcac
     2221 ggatggtcac ggtgtagaac gggggccaaa gactgtcaac ggcgtgaaat tcaatgacgc
     2281 aacgcagcat tcacgcaaac attaccgatt tttttgaaac cccttgcgga tcaatccccc
     2341 gccttccggc agtgatgaac tggtttgccg aagtttcatg caacgcaagc gaagggtcgg
     2401 agcgggacgc ggagtcgacg tggattcatt gaaaatttcc gaccgaaatc cttaccggta
     2461 acacacgaaa ctcccgacgc gggacggcgc ttgccggcaa cccgatcgcc tgcagcccgc
     2521 cgggcactgc ggcaacgtcc cggccggcgc ccggtccgcc ggcggcgctt cagcgcatca
     2581 gccagaactc ggcgctggcg ctctggccgg cggcatcgac gacggtcaca cgcgcggccc
     2641 cctccccgtc cggctgccag gcgacgctgc gccgcatcgg cgagccagtg agcggcacgc
     2701 cgttgaccag ccagcgcagc ggccgcaggc caccactcgc gaccagcggc agcggcgcac
     2761 cgtcggccgg caacgcaacg cgcgcgccgg gcagcggaaa gctcagcgcc agcgtcgcgc
     2821 gcgtgtcgcg ggcagccgag ccggccagat gccgccagtt cggcgccagc gcccggccgc
     2881 cgtccggcaa ctgagtctgc tcgacgaagg cgacgcgcgg gctcggggcc agcaggtcga
     2941 acacccgcca cagcagcggt gcggcggtgt tgcgcccgaa gtgccccggg ttcggcgagc
     3001 catccggccg gccgacccag acgccgaccg tatgcgcacc gtcgtaaccg agcgcccagg
     3061 cgtcgcgaaa gccgtaggag gtgccggtct tgtaagcgat gcggcgcggc tggcgcgtgt
     3121 cgccgggcgg cacggcctcg gccggcggcg gcgcgtcttc gaggatgcgc gcgagctgct
     3181 cggcggcgac cggcccgagc aggcgcatcg gcggccgggg cacggccgct accgcttcca
     3241 tcagcgtcag cggcggtgcg ctgccgccgt tggcgagcgc cgcgtacagg ccggcgagat
     3301 cccagagccg catgccggcg ccaccgagca cgatcggcag ccccggcgcg gcaccgtccg
     3361 gcagctgcag ttcgagcccg gcctcgcgca ggcgctgcgc gacgcgcagc ggcccgacgc
     3421 gatcgagcac cgccaccgcc ggcacgttca gcgagcgctg cagcgcctcg cgcacgctga
     3481 cctcgccgta gtagcgctcc atgaagttgc cgggcgaata gtcgccgaag cgcgtcggca
     3541 catcgtcgat gcgcgtgtcc ggatgcagca cgccgtcgtc gaaggcgagg ccgtagacca
     3601 gcggcttcag cgtcgagccc ggcgagcgca gcgcgcgcag cagatcgacg ctgccgtggc
     3661 gctgcggatc acggagctcg gcggcaccga cgtaggcgag cacgcgcccg ctgcgctgct
     3721 cgacgacgac gagcgcggca ttgacctgcg cccccagtcc ggcgagcgcg cgctgcgcga
     3781 gcgtcgcggt ctcggcctgc acgcgcgcat cgagcgtcga gtgcagttcg aaggcttgcg
     3841 gctgcgccgc gtgcagcgcg gcggcaagat gcggcaccgc gcgcggcagc gtcaggcgct
     3901 gtgccggcac ggcctcctcg cgcgcctcgg cagcccgggc ggcatcgagc acgccggccg
     3961 cggccaggcg catcagcacc ttgtcgcgcg ccaggcgtgc ggcctcgggc cggcgatccg
     4021 ggcgcagccg ggtcggcgac tgcggcagcg ccaccagcag cgcgtattcg gccggacgca
     4081 gctcgcgcgg cgacttgccg aaccacgcga ggctcgccgc gcgcaggcct tcgaggttgc
     4141 cgccgtacgg ggccagcgtc agatacatcg ccagcagctc gtccttggaa aagtgcgctt
     4201 cgagctgcag cgcgcgcagg cattcgatga gcttggcgcc gagcgtgcgc gggcgcggtt
     4261 cgagcaggcg cgcgagctgc atcgtgatcg tcgaaccgcc ggaaacgatc cgcccgtgcc
     4321 ggagccactg gccggcggcg cgcgcgagcg cgagcggatc gacgcccgga tgccggcgga
     4381 agcgctggtc ctcgaaggcg agcagcgccg cgacgtaggc cggatcgacc tcggcgagcc
     4441 gcaccggcag ccgccagtag ccatccgccg agcgctcgac gtgcagcagc gtgccgtcgg
     4501 cggcgagcac gcgcagggat tcgtgctgca ggcgcgacag ctcgggcggc agcaggcggt
     4561 cggccgtgac cagcgcgaag aagaccgcag ccagcagcag tacaccgata cgcgcgcgcc
     4621 acgaccacgg cccggcaacc ggccggtccg tcgtttgcct gccgcgcccc ctcccctgtc
     4681 ccgctccggg acaggggatg aaccgcaggc gggtgtgcgc cgccccccgc cacaccgggt
     4741 tccgcctcac tcgatcttca cccgccccat cgccccgcgc ccgcgcagct gcggccggta
     4801 catgtcctcg gcgagcacgg ccggcacgcg gtagtcgccg ggcgtgatgg cgcgcaccag
     4861 atacgcgagc gtgaagcggc gcgtgccgtc ctccagcccg atcgcggcga cgtagcggtc
     4921 atcgcgcagc tcgacgtgat cgggctcggt cagcgtcggc agccacggca gttcggacgg
     4981 tgcgcggttc gggccgaggg cggcgttttc gagttcgaag ccggccggca gcagatcgac
     5041 gaccagtccc tgcacgtact cgccggccgc gacctcgccg ccgatcacgg cgaccagcgt
     5101 cgtgccggcc ttgaccgggc ccgggccggg cggcagcggc ttgccgtcga gcgtgtacca
     5161 ctggcgcgtg accgtgatgc cctcgctcgc cgccggctgg tcggcgctcg ggatgccgct
     5221 gcgcgtcacg cgcacccagg cctgcgcctg cgcgtcgttg gcgagcgtga ggccgcgttc
     5281 gagcgctgcg gcttccggcc gcagccgcgc acgcgccttg ccctgcgccc acggcgcgcc
     5341 gtcgcggctg atcgcaagct ggccctgacc ggcgccgagc gcctgcgcag cgagcagcag
     5401 ccaggcctgc tcctgcgtgc tgaggtagcg ccggccgtcg gcctcgcgcg cgacatcggt
     5461 ggccagatcg ccgatgcgtg cgctctggcc ggcctcggtg gcgagcgcga gcagggcggc
     5521 gcggtcacgc agcgcactgc cgtaatccca ccagtaatac ggccgcgcct gcagcgcgaa
     5581 ggcatggtcg aaggccgcgc gcgcgcgctc gctgtcgccg tagcgcgcca gcgccgcgcc
     5641 gagctgcgcg gccgccagct cggtcggcat cttcgcgagc caggcatcgt gcaggtagcg
     5701 cagctcgccg ggcgtggccg cctgcgcgcg cgccagcacg taggcactgg ccgcgcgcgt
     5761 cgcgagttcg cgctcgtccg ggtcgctgcg gctgttcagc gcatcgtgca gcgccgccag
     5821 cccgtgcttc agcgccacct ccggcaccag atagccctgc tccttcgcgc gcacgaggaa
     5881 gttcatgacg taggcgttca gccacggatt cggatcatcg agcgcgcgcc acagcccgaa
     5941 gccgccatcg ctcctctgct gcgcgagcag ccgggcgatg gcgtcctgca cgcgcttcgg
     6001 cagcgccgga tcgaccgcct cgccgccgag ccagtcgcgg gcgagatcgt tggcgtacaa
     6061 cagcggcagc gcgcggctgg tggtctgctc ggcgcagcca tacggatagc gatcgagcgc
     6121 atcgaggagc gccggcacgt cgatcggcgg ccgtgacgag gcgagcacgt cgacgcgtcc
     6181 gctgcccggc agccagccgc cgagcagatc ggcgccgagt gcgagcttcg cgcccggcgc
     6241 gagcgccttc gtctcggcga aggtctgcac ggcctgcgcg gcgcgcaccg tgaacggcca
     6301 ttcgcgttcg atgcggctgc cgtccgggcc ggtcacggtc agcgtcaggc gcgcatcgcc
     6361 aacgccggtg gcactgagcg caagcgcgcc gcgcacctgc ttcggccggc cgttgccgag
     6421 cgcgagcgtc tgcgcaggcg agccttcgat cgtcaccggc ccgctgacgg cgtactgcac
     6481 ctgccagtcg ccgggcacag cggcgagatc atcgaactgc gtgcgcacct gcgcgcggtc
     6541 gccgggcgcg aggaagcgcg gcagataggc ttcggcgacc agcggatcac gcaccggcac
     6601 ctgtgcctcg ccgcgaccga cgcgcgtgcg gtcccaggcg accgccatca gccgcagccg
     6661 gccgttgaag tccggcagcg ccagcggcac gctcacgcgc ccctgcgcat ccggcgtcac
     6721 gaagcccgag aacagcgcga cgctctgccg gtgcttgagc tgcgcgccgt cgagcaggcg
     6781 gctgtcggcg tcgccgccgc tgcgcagttc gccggcacgg ccctcggccg gtgcgatcag
     6841 ccggccgtag acatcgagca tgtcgagtcc gagccggcgc ttgccgagat accaggcgac
     6901 cgggtccggc gtctcgaagt cggtgagctg caggatgccc tcatcgaccg cggcgacggt
     6961 cagcgtcacg cccttgccgc cgctgctgcc atcggcgccg ctgagcgtca ccggcagctg
     7021 cacggtctgg cgcggccgcc attcggccgg cgcatcgagc ttcacgtcga gccggcgcgg
     7081 cgcgtcatcg agtgcgagcc aagcaagccc gatcgcccgg cccggcccga ccgccggcgc
     7141 ctcgcgcccg gcccggaacg ccgtcgcgct cacgtacacg cccggtcccc agtcggcatc
     7201 gaccggcaga tcgatggtcg cgccctcggc cggcacgtac tgcgagtgcg cgagccagag
     7261 cttgtggctc acgaccgtca gcagcacctc accggcaaac ggcggctgca cgcgcacctt
     7321 cgcgctcgcg cccgcggcgt agcgtggctg atcgagcgtc acgcgcagct gatcgggccg
     7381 gtcgccggcg ctcggggcgg cgtaccagcc gaccgtgaag cgcacgctgc tcgccgtcgt
     7441 gccggccggg tcgtagacct ccagccggta cgggccgtag tcgagtgcag ccgtgttgag
     7501 cttcgccggc gcgtccatcc cgatgtcgag cgcgccccct gcgaccggcg cgccgtcgcg
     7561 cagcagcagc ttgtagtcga agcggccggt gtcgccgttg tggaaccagt agtaatccca
     7621 gttctcgcgg tacagctccc agcgcaggcc tttggcctcg cgcaccttgc cgtcggcccc
     7681 gaccgcgatc agctcgaacg gcgctgccgc gccctgcttc aggccctgcc cggcgtcaaa
     7741 gccggggcgg atgccgagcg catacggtgt gctccggtac ggcaggcgca cgacgcggcg
     7801 caccggccgt ccgcccggtt cctcgatgcc gacgcgcacg ctcaggcgca gcggccggcc
     7861 ggtatcgggc accggcggcg cctcgatgcg cgccaccgcc cggccctcct cgtcggtggc
     7921 ctgcggcaga ccttccaggt tctggtaggc cggttcccat tcgtcctgca ccagtccgaa
     7981 gcggtagccc gcgaattgcg gatacggatc gggatcggct tcgagcgtcg cgtccatctc
     8041 gaccggcagg ccggcggccg gcgcgccgta caggaactgg ccgctgatcg cgagcacgac
     8101 cgccgaatcc ggcgtcagga acggctggtc ggtggcgagt tccaccttga tctgctgcgg
     8161 cacgaagtcc tcgacctgga agcgccgctc gccgatcgcc ggcccgtccg gctcggcgtg
     8221 caggcgcgcg gtccaggtgc cgctgcgggc gttggccggc agttcgaggc gggtgtgata
     8281 cgcgcccggt gcgccggggg cgagcacgcg ccgctcggcc tcctcgccgt cggggcggcg
     8341 cacgatcagc gtcaccggca ggttctcgac cgcgctcgcg ccggcatcgc gcagcagcgc
     8401 gctcagctcc accgcctcgc cgggccggta gacgccgcgc tccgtataca ggaaggcatc
     8461 gagcccgacc ggctgcacgc ggccatcgac gccgcgatcg gacagatcaa acgccggctg
     8521 gccgagatcg agcaggttga agtcaccgcc cggcccgtag gcatagaggc cgcgcaccgc
     8581 gcgcccgccg ctggcgcgca gcaggccggg atcgaacttc gcgcgcccct cggcatcgct
     8641 ggtcacggtc gcgagttcgg tgttgttgcg cgcgagcagc cgcagttcga cgttcggcgc
     8701 cggccggccg gattcgagcg aacgcacgaa cacgtgcagg ccatcggcgc cgcgcagcac
     8761 gctcaggccg aggtccgaga cgatcagcca ctgcgtcgcg tgcacgtcgt agtcgtagtc
     8821 atcgtcctcg tcgccggcgg cgggggcctg cttgcgcggc gggcgggcgg tcagcacgta
     8881 gacgccgggt ttcgtctccg gcagcacggt cttgagcggc agcagcgtca ccgtctcgcg
     8941 gttcttgtcg ccggcgaact cgacgctgcc gtgccagacc tcctcgccgc ggtcgctggc
     9001 gatcgcatcc tcgtcggagc cgtagagcgc ctcgtcgaag ctgccgttga ggacttcgcg
     9061 gatcaggttg cggtcgttga tgcgcagcag cttcagttcc gccgccggca ggttgacgct
     9121 gaccaccggc agcatctgcc cggccttgcg cggcagcacg tagccgttgc cacggaaggc
     9181 cacactcggc ttgcggtccg ggaacgccag cgtgaaggtc tcgctgctgg ccgtgcgatc
     9241 gcctttcgcc cccggcacgc cggcgcgcac cgtcacccgg tagccttgtc cccactcgac
     9301 accctcggcg cagagcttgg tgtcgcgcac cgtcaccgac agccgcaccg ccggctcggt
     9361 ctgcaggaag tcctcgtagt ggtactggcg ggccttcgaa agcgggtcgc tgaattcgac
     9421 gcagaagcgc ggcgtcgcgg actcttcctc caccgccgcg ttgaccggct tgatgatgag
     9481 cgtcgcgcgc aggttggcgg cgcgcctggc gatgccctcg tccggcacca gcgccagccc
     9541 ctcgtcatac atcgcgagcg ccgcgcgccc gtcctgatta acctcggtca gccgcccgag
     9601 cacgaacagc gccgccgcct gttcctcggc gctcttggcg aggcgcagcg ccgcatacgc
     9661 cgccggccgc gcgcgctcgt cctgcggatt gctgttgccg cgggcgcgcg cgagcttcag
     9721 ccacagcggc ccgtcatcgg ggtgcgcgcg caccagcagc tcaagcggca cgatcgcctc
     9781 cccccacttc ttcgccttca acgccgcatc ggccttcgcc tgcgtatccg gcggcagcac
     9841 cttgccggcc tgcgcgatca gcgccgcccg gtactgatcg gccgcgacgc cgagggtctt
     9901 gtcttcaaag gcatgggcag gcatggcgga cagccacgca aacagcaggc acagcagcgg
     9961 cagggcacgc atggtgaagg gattccctgg ggagcgtatg gacagaagcg gaggatgccg
    10021 cgcaggggcg ggacggcggg taaggatagg cggtcgggat gggggcggga gtacggcggg
    10081 tgacgggtgg aaattgtcgc gggggtgagg cagtatcgag gggcaatgtc gcagacaggg
    10141 agaggctaga tgtcgatcga atacgatttc gaccgtctat tggagagagg tggatcgggt
    10201 ctacatcgct gaagtaccgg aactgcccgg gtgcatggcg gatggggcca gccaagagga
    10261 ggctgtggcg aatgcacgac aggttattga gtactggatt gctacggcga aggaacttgg
    10321 gcacgcagcg ccggagccaa gagggt
//
LOCUS       DAOQIE010000121        10293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3273_length_10293_cov_7.519047, whole genome shotgun sequence.
ACCESSION   DAOQIE010000121 DAOQIE010000000
VERSION     DAOQIE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_10293_cov_7.519047"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..416)
                     /locus_tag="PK144_09075"
     CDS             complement(165..416)
                     /locus_tag="PK144_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65918.1"
                     /translation="MRILLANLPADADAGAVTAFLAGHGMADATDVTLVPGSGSEPAA
                     TFELPVSAAVAETVALHLSGKAWQGQVLRATVFVTPAGD"
     gene            complement(544..2907)
                     /locus_tag="PK144_09080"
     CDS             complement(544..2907)
                     /locus_tag="PK144_09080"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HNF65919.1"
                     /translation="MSQTNPNLGQPAPAGLPERLQALADRWSDWQDELGGVLGHDLQR
                     VVAAAAARQSRRCDQAARLATAAWQPGQTAGVLHYAVDSLQRTLLFWDTLRERGNDFI
                     ELYRAGNPPLLAFDYETLIDGRNFDEQPVNYALVRILPPAGVTIDARKRPYLIVDPRA
                     GHGAGIGGFKPESQVGVALRAGHPVYFVIFFRDPVPGQRLRDVAAAEARFLAEVVRRH
                     PDSDKPCVIGNCQGGWAVMGMVAAHPDAAGPLVINGAPMSYWAGTTGHNPMRYSGGML
                     GGSWLAQLTSDLAAGSFDGAWLVQNFENLDPAHNYWQKFYKLYAEVDTEASRFRDFEK
                     WWGGYVTLTGSEIRAIVDNLFIGNRLAAGEIPLDRGETLDLRRIRSPIVVFCSEGDNI
                     TPPQQALNWILDVYRDDATLRAFGQTIVYLRHLKVGHLGVFVSGSVARKEYTEIVGTL
                     DAIERLPPGLYEMLIDEDGRTPAGDPRYRVELAQRSMSDIVALDTDSRREEDYFRVVA
                     ALSELNAKAYDLLASPVLQALAVPESVELQKMMHPLRAGHWAFSDWNPWLTALGPWVS
                     WARSMRAPVEAGHPLRQLEQLWVDGIGDLFDLYRDTRDMSVELTFYGLYGFLNVLGIP
                     KPGERERSATSDAERVQREIAAWVDGHIASGGYLEGYARMALLLFHAQGGIGRDGFVQ
                     VLERLDAMPEVAALDREARRRIVREQSLIIAHAPERALATLAELLVTPGERERALAAL
                     DALFAGEALSDAAAALRKLLRSTFDSGTSGPVRSGSPGRSRKSSPAA"
     gene            complement(3021..3269)
                     /locus_tag="PK144_09085"
     CDS             complement(3021..3269)
                     /locus_tag="PK144_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65920.1"
                     /translation="MKITLTHIPETADIDAVRAFLREHGIDDAGEIVLIEGDGSLPAV
                     AFELEVSAAVAESIAAHLRGKLWDGHVIGAGLLLMSGH"
     gene            3591..4115
                     /locus_tag="PK144_09090"
     CDS             3591..4115
                     /locus_tag="PK144_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNF65921.1"
                     /translation="MAKYKVQPSGRERFFPENDIIVSKTDLKGHITYANRVFLDIAGL
                     DEDEALGEPHCLIRHPDMPRCVFRLLWDTIAGGSECFAYVKNMAVNGDHYWVLAHVTP
                     TFGPGGDIIGYHSNRRVPRRDAVAKAEALYRTLLAEEQRHTDRSAGLAASYALMQRLL
                     AEQGVAYDEFVLGL"
     gene            4093..5328
                     /locus_tag="PK144_09095"
     CDS             4093..5328
                     /locus_tag="PK144_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF65922.1"
                     /translation="MNSSSVSEAVARTDATELETLRAEVARYRHWMSEVTRVCRSAAD
                     GDLEPRLIHIDAEGDLAMMLHSINRMLDMSDALLRESSATLDYASHDKYFRPILPHGL
                     RGSFKRTAEQINGAIGEMQRKSVSLHTAEDRRQKLAHSFEASIRGVAQTLAAAVTELA
                     STAESLKRIADLTVDRATSAAAASTQTAERMSRIAESTHGLAESIGDIGRRVSASTEL
                     AHQTAQDVQRADGTVRELAGCTSSIGRVSGLIQKIAGQTRLLALNATIEAVRAGSAGR
                     SFAVVASEVKALSNQTAGATDDIGNQINRIQGATDEVVRSIADITRSITDIDRNSSSI
                     SSTVQEQMRATDDISSAVRAAADGSRAVSADLQEMMEAATGTTSAADELLVAARELSH
                     MAEQLNAEVDDFVHHLYSS"
     gene            5418..6059
                     /locus_tag="PK144_09100"
     CDS             5418..6059
                     /locus_tag="PK144_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNF65923.1"
                     /translation="MLGIFHVTLTELLALHGGPGAVARLLEQAGLPAGTRFRIDTCYP
                     DTDAERLLATAAQVLGLDAAALHRAYAAHAGAWALRVFPSFFELAPDSREFLRSQPRF
                     AGLLDAGAQCAGPDPLPAGAPGPIEIETPAPDALRIRWRASPGLLALYRELLRWVVRH
                     YRDVADVRMQVNGSGTGMVAIYDLSWIRFAGDTRRHERFETTAPDESPVPPAA"
     gene            6031..7245
                     /locus_tag="PK144_09105"
     CDS             6031..7245
                     /locus_tag="PK144_09105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNF65924.1"
                     /translation="MSPLFPPPPEAGPGDDAMRLEIEAIADGVCRVLDGDFSIRVGTG
                     SADHTIQKLAMLINFLLATGERQTERLNRMNALLEARVAARTRELEEAQMQLRRALGR
                     LEATHAHSEALFDAALDVIITIDTGGIIRSANRATSATLGYEPGELLGRNVSVLMPAP
                     MRDEHDAYLHRYLATGEARVINVGREVTVRRRDGSTYLAFLTISEYRIGGRPYFVGFL
                     RDIAYFKQREQQLRRLAELDPLTGVPNRSGIIYALERLLTETGEQAPGLVGLFYIDLD
                     DFKPVNDTLGHDYGDALLIAVAERLRRRVRGSDLVGRLGGDEFVVVAGPVPTVGDIEH
                     IRVTLAEALAQPFEVLDRELGLGGSIGVALYPRDGTTPEKLLRVADQSMYATKRRRKQ
                     RRHGPAPDGQAG"
     gene            complement(7242..7772)
                     /locus_tag="PK144_09110"
     CDS             complement(7242..7772)
                     /locus_tag="PK144_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF65925.1"
                     /translation="MSEEWFDVVDEHDRPLGRALRAEVHRLGLRHRSVHMLVFNAAGS
                     VFLQKRSLAKDNCPGLWDVSAAGHVGAGEDYDAAAPRELAEELGCVPAVAPELLFALE
                     AAEHTGWEFVRVYRVEAEGPFELDPVEIAEGRWFTPAEVDAWLAGRAAETTATLRIIW
                     SRVRAAAAGLPGADGR"
     gene            8167..9426
                     /gene="amt"
                     /locus_tag="PK144_09115"
     CDS             8167..9426
                     /gene="amt"
                     /locus_tag="PK144_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNF65926.1"
                     /translation="MTPQALAATDPAALKLALDTVWLVTTGALVFFMQAGFALLESGQ
                     SRAKNAINVIMKNYTDCCFGAFAFWALGYGLMFGKSPDGWLGRDHFLLGNAEQLDYSL
                     LFFQTMFAATAATIVSGALAERTHYWAYITGSIVITGVIYPVFGAWAWGSLYEGTGWL
                     KQIGFIDFAGSTVVHSVGGWCALAGILVVGPRLGRFGPNGEPRTIPGHNLGSVALGGF
                     ILWLGWFGFNGGSTAVAEAGIGRIVLNTHFGAAAGAIGTIAMQRLLQAPVLLTATVNG
                     SIAGLVSVTAGCATMEPLHAMFTGLIAGMLMVASERAMLHLRLDDVVGAVPVHGVCGA
                     WGTLAAGLFYAPHLFDPTRVTVQVIGICTAFIWAFPSAWLMFKAIDLTIGLRARGIAE
                     QRGLDYAEHHEQGYPEFQGIALHEEIR"
     gene            9428..10072
                     /locus_tag="PK144_09120"
     CDS             9428..10072
                     /locus_tag="PK144_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65927.1"
                     /translation="MAAVHDFQHDPGFAEQQDALSTLLESLLSPEGVRAALTVWSRDY
                     AHRPGALRAYANCLVREFGVPLGLREMHRQLVQHLLLPATRRGPVLLAAAAQTPDRAV
                     PALTRAFVLLVGALLARCGQQDPKAATALRSALVGAAMTAKLSRADRELLVAWLDGSG
                     PVPARAYDEALLRELVHAAWAGLCTLWGPPVADRQLGAAVHAAEEQCAEVRRLL"
     gene            10029..>10293
                     /locus_tag="PK144_09125"
     CDS             10029..>10293
                     /locus_tag="PK144_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65928.1"
                     /translation="MPPRSSAPRSGACSERRRARAGARGSRGGSAYPCAPMSDPALRI
                     LRTVFGYDAFRGAQAEIVAHVAGGGDALVLMPTGGGKSLCYQIP"
BASE COUNT         1566 a         3685 c         3573 g         1469 t
ORIGIN      
        1 cgcggatggt gcgggcgacg gcgtaggcga tcgaactctc gttggcgatg ccgaccacca
       61 ggccgcgcct gccgtgcagg ctgaagggat tgctcatcag tcgtgttcct catcccgttg
      121 tgatcctgac ccggccgggg ccgggccgct tgtggagggc gtgctcagtc cccggccggc
      181 gtcacgaaca cggtcgcgcg cagcacctgg ccctgccagg ccttgccgct caggtgcagg
      241 gcaacggtct cggcgaccgc ggcactgacc ggcagctcga aggtggcggc cggctccgag
      301 ccggagccgg gcacgagggt gacatcggtg gcatcggcca tgccgtgtcc ggcgagaaag
      361 gccgtgacgg ccccggcatc ggcgtcggcg ggaaggttgg caagcaggat gcgcatggtg
      421 cggactccgg aggaagccaa ggagcggaac gaccggcgcg cctgcgggcg cgccggtccg
      481 gctgacgaaa gcccgccggc ggaaaagacg gcgggctggc aagaaaaacg gaaaccggcg
      541 tgttcaggcg gccggtgacg atttgcggga acgtccggga gaacccgacc gtaccgggcc
      601 tgaggtcccg gaatcgaagg tgctgcgcag cagcttgcgc agcgcggccg ccgcatccga
      661 cagcgcctcg ccggcgaaca gcgcatcgag cgcggcgagg gcccgctcgc gctccccggg
      721 cgtgaccagc agttccgcca gcgtggccag cgcgcgctcg ggcgcgtgcg cgatgatcag
      781 gctctgctcg cgcacgatgc gccggcgcgc ctcgcgatcg agcgcggcga cctcgggcat
      841 cgcatcgagg cgctccagca cctgcacgaa gccgtcgcgg ccgatgcccc cctgcgcgtg
      901 gaacagcagc agcgccatgc gcgcgtagcc ttcgaggtat ccgccgctgg cgatatggcc
      961 atcgacccag gccgcgatct cgcgctgcac gcgctcggca tcgctggtgg cgctgcgctc
     1021 gcgctcgccc ggtttcggga tgccgagcac gttcaggaag ccgtagaggc cgtagaacgt
     1081 cagctcgacg ctcatgtcgc gcgtgtcgcg gtacaggtcg aacaggtcgc cgatgccgtc
     1141 gacccagagc tgttcgagct ggcgcagcgg atgtccggct tcgaccggtg cgcgcatcga
     1201 gcgcgcccag ctcacccacg ggccgagcgc cgtgagccac gggttccagt cgctgaaggc
     1261 ccagtgaccg gcgcgcagcg gatgcatcat cttctgcagc tcgacgctct cgggcacggc
     1321 cagcgcctgc agcaccggcg atgcgagcag gtcgtaggcc ttcgcgttca gctccgacag
     1381 cgcggcgacg acgcggaagt agtcctcctc gcggcgcgag tcggtgtcga gcgcgacgat
     1441 gtcgctcatc gaacgctgcg cgagttcgac gcgatagcgc ggatcgccgg ccggcgtgcg
     1501 gccgtcctcg tcgatcagca tctcgtagag gccgggcggc aggcgctcga tggcatcgag
     1561 cgtgccgacg atctcggtgt attccttgcg cgcgaccgaa ccggagacga acacgccgag
     1621 gtggccgacc ttcaggtggc gcagatagac gatggtctgg ccgaaggcac gcagcgtcgc
     1681 atcgtcgcgg tagacatcga ggatccagtt cagcgcctgc tgcggcggcg tgatgttgtc
     1741 gccctcggag cagaacacca cgatcggcga gcggatgcgg cgcaggtcca gggtctcgcc
     1801 gcgatcgagc ggaatctcgc cggccgcgag ccggttgccg atgaacaggt tgtcgacgat
     1861 ggcgcggatc tcggagccgg tcagcgtcac gtagccgccc caccacttct cgaagtcgcg
     1921 aaagcgcgag gcctcggtgt cgacctcggc gtagagcttg tagaacttct gccagtagtt
     1981 gtgcgccgga tcgaggttct cgaagttctg caccagccag gcgccgtcga agctgccggc
     2041 agccagatcg ctggtcagct gcgccagcca gctgccgccg agcatgccgc cgctgtagcg
     2101 catcgggttg tgcccggtcg tgccggccca gtacgacatc ggcgcgccgt tgatgaccag
     2161 cggcccggcg gcatcgggat gcgcggcaac catgcccatc accgcccagc cgccctggca
     2221 gttgccgatc acgcagggct tgtcgctgtc cggatggcgg cgcacgacct cggcgaggaa
     2281 gcgcgcctcg gcggcggcca cgtcgcgcag ccgctgaccc ggcaccggat cgcggaagaa
     2341 gatcacgaag tacaccggat ggccggcccg cagcgcgacg ccgacctgcg attcgggctt
     2401 gaagccgccg atgccggcac cgtggccggc acgcggatcg acgatcaggt acgggcgctt
     2461 gcgtgcatcg atcgtgacgc cggccggcgg caggatgcgc accagcgcgt agttgaccgg
     2521 ctgctcgtcg aagttgcgcc cgtcgatcag ggtctcgtag tcgaaggcga gcagcggcgg
     2581 attgccggca cggtacagct cgatgaagtc gttgccgcgc tcgcgcagcg tgtcccagaa
     2641 cagcagcgtg cgctgcagcg agtcgacggc gtagtgcagg acgcctgccg tctgccccgg
     2701 ctgccatgcc gcggttgcga gacgtgcggc ctgatcgcag cggcgcgact gacgcgcggc
     2761 ggcggcggcc acgacgcgct gcaggtcatg gccgagcacg ccgccaagtt cgtcctgcca
     2821 gtccgaccag cggtcggcga gcgcctgcag gcgttccggc agtccggccg gcgccggctg
     2881 cccgaggttc ggattcgtct gactcatgag ctgaccctca cgggagatga caggagaagc
     2941 accgcgccgg cctgccggtg cagtgcgatg aaggcaggat ttcgcagcaa tgcggcggct
     3001 cgtgcggtgc aggccggccg tcagtgaccg gacatcagca gcaggccggc gccgatgacg
     3061 tggccgtccc agagcttgcc gcgcagatgc gcggcgatgg actcggcgac cgcggcactg
     3121 acctcgagct cgaaggccac ggccggcaga ctgccgtcgc cctcgatcag cacgatctcg
     3181 ccggcatcgt cgatgccgtg ctcgcgcagg aaggcgcgca cggcatcgat gtcggcggtt
     3241 tcggggatgt gggtcagggt gatcttcatc aggcggtttc cctgcaggac gggttcggca
     3301 gcgcacggaa gcggtcccgg caggacctgc aaacaccggc gcggacctca cgcagcctag
     3361 cagtgcagcg aaaaactgcc ctgccctgca gcccgcgcgc aagcggccga tatggggcag
     3421 tgaactcgcg tgtcgaccgc tgccgcgatg ccgtcggcac cgggcggggc atggaggcat
     3481 tgcatagact gccggctggc cgcgcaccgg ccgcgcttcc tatagcggtc tgctggctca
     3541 ccaaaccaac atccggcgcc gccgatgccg cggacgcggg ggagagagcg atggcgaagt
     3601 acaaggtcca gcccagcggg cgtgaacggt ttttccctga aaacgacatc atcgtttcga
     3661 aaaccgacct gaaagggcac atcacctacg caaaccgcgt gttcctcgac atcgccggtc
     3721 tcgacgagga cgaggccctg ggcgaaccgc actgcctgat ccgccacccc gacatgccgc
     3781 gctgcgtgtt ccggctgctg tgggacacca tcgccggcgg cagcgaatgc ttcgcctacg
     3841 tcaagaacat ggccgtcaac ggcgatcact actgggtgct cgcccacgtg acgccgacct
     3901 tcggccccgg cggcgacatc atcggctacc actccaaccg gcgcgtgccg cggcgcgacg
     3961 ccgtcgccaa ggccgaagcg ctgtaccgca cgctgctcgc cgaagagcag cgccacaccg
     4021 accgcagtgc cggtctcgcc gcctcgtatg cgctgatgca gcgcctgctc gccgaacagg
     4081 gagtcgccta cgatgaattc gtcctcggtc tctgaagcgg tcgcgcgcac ggacgccacc
     4141 gagctggaga cgctgcgtgc cgaagtcgcg cgctaccggc actggatgag cgaggtcacg
     4201 cgcgtctgcc gctcggcggc cgacggggat ctcgaaccgc ggctgatcca catcgacgcc
     4261 gaaggcgacc tcgccatgat gctgcacagc atcaaccgca tgctcgacat gagcgacgcg
     4321 ctgctgcgcg aatcgagtgc gacactcgat tacgcgagcc acgacaaata cttccggccg
     4381 atcctgccgc acggcctgcg cggcagcttc aagcgcacgg ccgaacagat caacggcgcg
     4441 atcggcgaga tgcagcgcaa gagcgtctcg ctgcacacgg ccgaggatcg ccgccagaag
     4501 ctcgcgcatt ccttcgaggc ctcgatccgc ggcgtcgcgc agacgctggc ggcggcggtc
     4561 accgagcttg cctcgaccgc cgaatcgctc aagcgcatcg ccgacctgac cgtcgatcgc
     4621 gccaccagcg ccgcggcggc ctcgacgcag accgccgagc gcatgagccg catcgccgag
     4681 tccacgcacg gtctggccga gtcgatcggt gacatcggcc ggcgcgtcag cgcctcgacc
     4741 gaactcgcgc accagaccgc gcaggacgtg cagcgcgccg acggcaccgt gcgcgaactc
     4801 gccggctgca ccagcagcat cggccgcgtc agcggcctga tccagaagat cgccggccag
     4861 acgcggctgc tggcgctgaa cgcgacgatc gaggccgtgc gcgccggcag cgccgggcgc
     4921 agcttcgcgg tggtcgcctc ggaggtgaag gcgctgtcga accagaccgc cggggccacc
     4981 gacgacatcg gcaaccagat caaccgcatc cagggcgcga ccgacgaggt cgtgcgctcg
     5041 atcgccgaca tcacgcgctc gatcaccgac atcgaccgca actcctcgtc gatctcgagc
     5101 acggtgcagg agcagatgcg cgcgaccgat gacatcagct ccgccgtgcg cgccgccgcc
     5161 gacggctcgc gcgcggtgtc ggccgatctg caggagatga tggaagccgc gaccggcacg
     5221 acctcggccg ccgacgaact gctggtggcg gcgcgcgagc tgtcgcacat ggccgagcag
     5281 ctcaacgccg aggtcgacga cttcgtgcat cacctgtaca gcagctgagc cggcgacttc
     5341 cgtaacgccc ccgggcagcg ccgcgttacc atgcggtaat gtttcggggg cgccgtcgcg
     5401 ctccgttcga gggagcgatg ctcgggatct tccacgtcac gctgacggaa ctgctcgccc
     5461 tgcacggtgg cccgggcgcc gtcgcgcggc tgctcgaaca ggccggcctg ccggccggca
     5521 cgcgctttcg catcgatacc tgctatccgg acaccgacgc cgaacggctg ctcgccaccg
     5581 ccgcgcaggt gctggggctc gacgccgcgg cgctgcaccg ggcctatgcc gcgcacgccg
     5641 gtgcctgggc gctgcgggtg ttcccgagct ttttcgaact ggcgccggat tcgcgcgagt
     5701 tcctgcgcag ccagccgcgc ttcgccggac tgctggatgc cggcgcgcag tgcgccgggc
     5761 cggacccgct gccggccggc gcccccggac cgatcgagat cgaaaccccg gccccggatg
     5821 cgctgcgcat ccgctggcgc gccagccccg gcctgctggc gctgtatcgc gaactgctgc
     5881 gctgggtcgt gcgccattac cgcgacgtgg ccgacgtgcg catgcaggtg aacggcagcg
     5941 gcaccggcat ggtcgccatc tacgatctgt cctggatccg ctttgccggg gacacgcgca
     6001 ggcatgagcg cttcgagacc accgcacccg atgagtcccc tgttcccccc gccgcctgag
     6061 gccggccccg gggacgacgc gatgcgcctc gaaatcgagg cgatcgccga cggggtctgc
     6121 cgcgtgctcg acggcgactt ctcgatccgg gtcggcaccg gttcggccga tcacacgatc
     6181 cagaagctgg cgatgctgat caacttcctg ctggccaccg gcgagcgcca gaccgagcgg
     6241 ctcaaccgca tgaacgcgct gctcgaagcg cgcgtcgcgg cgcgcacgcg cgagctggag
     6301 gaagcccaga tgcagctgcg ccgcgcgctc ggacggctcg aagccacgca cgcgcacagc
     6361 gaggcgctgt tcgacgccgc gctcgacgtg atcatcacca tcgacaccgg cggcatcatc
     6421 cgctcggcca accgcgccac cagcgccacg ctcggctacg agccgggcga gctgctcggg
     6481 cgcaacgtca gcgtgctgat gccggcgccg atgcgcgacg agcacgacgc ttacctgcac
     6541 cgttatctcg ccaccggcga ggcgcgcgtg atcaacgtcg ggcgcgaggt gacggtgcgc
     6601 cggcgcgacg gcagcaccta tctggccttc ctgaccatca gcgagtaccg catcggcggg
     6661 cggccgtact tcgtcggctt cctgcgcgac atcgcctact tcaagcagcg cgagcagcag
     6721 ctgcgccggc tcgccgaact cgacccgctg accggcgtgc cgaaccgcag cggcatcatc
     6781 tacgcgctgg agcgcctgct caccgaaacc ggcgaacaag cgccggggct ggtcgggctg
     6841 ttctacatcg atctcgacga cttcaagccg gtcaacgaca cgctcggcca cgactacggc
     6901 gatgcgctgc tgatcgcggt cgccgagcgg ctgcggcgcc gggtgcgcgg ctccgatctg
     6961 gtcgggcggc tcggcgggga tgaattcgtc gtggtcgccg gcccggtgcc gacggtcggc
     7021 gacatcgagc acatccgcgt cacgctcgcc gaagcgctgg cgcaaccctt cgaggtgctc
     7081 gatcgcgaac tcggcctcgg cggcagcatc ggtgttgcgc tgtacccgcg cgacggcacg
     7141 acgccggaga agctcctgcg cgtcgccgac cagtcgatgt atgcgaccaa gcgccggcgc
     7201 aagcagcgcc gtcacggccc ggcgccggac ggtcaggccg gctagcggcc gtcggccccg
     7261 ggcaggccgg cggcggccgc acgcacgcgc gaccagatga tgcgcagcgt cgccgtggtt
     7321 tcggcggcgc gccccgccag ccaggcatcg acctcggccg gcgtgaacca gcggccctcg
     7381 gcgatctcga ccgggtccag ctcgaacggc ccttcggcct cgacccggta gacgcgcacg
     7441 aactcccagc cggtgtgctc ggcagcctcg agcgcaaaca gcagctcggg cgccacggcc
     7501 ggcacgcagc cgagctcctc ggccagctcg cgcggggcgg ccgcgtcata gtcctcgccg
     7561 gccccgacgt ggccggccgc cgagacatcc cagagccccg gacagttgtc cttggcgagc
     7621 gagcgcttct gcaggaacac cgagccggcg gcgttgaaca ccagcatgtg cacggaccgg
     7681 tgacgcagcc cgagccggtg aacctcggcg cgcagcgcgc gcccgagcgg ccggtcgtgc
     7741 tcgtcgacca cgtcgaacca ttcctcggac atcacgtttc accctgcgcc ccggacggcg
     7801 gcaacgccgc accttgccac ggcgggccgg caggcgccta gcaggaacgg cacagaccgt
     7861 gtatggacgt aatttaattg cgggctctcc ggatgccatc cgggcggccc gcacgaatga
     7921 aatccagata aaacatgaat ttgaaccagc acctgcgaaa cccgggcacg gggctgcgga
     7981 ttttccggtc cgaccccggc aggcggcgta tgcgcacgtc cggcgggcgc gggtagcatg
     8041 cgggcgcatc gaccggtcgc tgcggcagcg gcgcaccggc gagcccggtt tcccttcccg
     8101 cacggacgat tcggacatgc tgccccgccc cccttcccgg ccctcgccgc cctcgcgctg
     8161 ctgacggtga ctccgcaggc gctcgccgcc accgatccgg ccgccctgaa actcgcgctc
     8221 gataccgtct ggctggtgac caccggcgcg ctggtgttct tcatgcaggc cggcttcgcg
     8281 ctgctcgaaa gcggccagtc gcgcgccaag aacgccatca acgtcatcat gaagaactac
     8341 accgactgct gcttcggggc gttcgcgttc tgggcgctcg gttacggcct gatgttcggc
     8401 aaaagccccg acggctggct cgggcgcgat cacttcctgc tcggcaacgc cgaacagctc
     8461 gactacagcc tgctgttctt ccagaccatg ttcgcggcca cggccgccac catcgtcagc
     8521 ggcgcgctcg ccgagcgcac gcactactgg gcctacatca ccggttcgat cgtcatcacc
     8581 ggcgtcatct atccggtgtt cggcgcctgg gcctggggca gcctgtacga agggaccggc
     8641 tggctgaagc agatcggctt catcgacttc gccggctcga ccgtcgtgca ttcggtcggc
     8701 ggctggtgcg cgctcgccgg catcctcgtg gtcgggccgc ggctcgggcg cttcgggccg
     8761 aacggcgagc cgcgcacgat ccccgggcac aacctcggct cggtcgcgct cggcggcttc
     8821 atcctgtggc tcggctggtt cggcttcaac ggcggctcga ccgccgtggc cgaggccggc
     8881 atcggccgca tcgtgctgaa cacgcacttc ggggccgccg ccggcgcgat cggcacgatc
     8941 gcgatgcagc gcctgctgca ggccccggtg ctgctgacgg cgaccgtcaa cggctcgatc
     9001 gccggcctgg tgtcggtgac ggccggctgc gcgaccatgg agccgctgca tgcgatgttc
     9061 accggcctga tcgccgggat gctgatggtc gcctccgaac gggcgatgct gcacctgcgg
     9121 ctcgacgacg tggtcggtgc cgtgccggta cacggcgtct gcggcgcctg gggcacgctc
     9181 gccgccggtc tgttctatgc cccgcacctg ttcgatccga cccgcgtcac cgtgcaggtc
     9241 atcggcatct gcacggcctt catctgggcc ttcccgagcg cctggctgat gttcaaggcc
     9301 atcgacctga cgatcggcct gcgcgcgcgc ggcatcgccg agcagcgcgg tctcgattac
     9361 gccgaacatc acgaacaggg ctatccggaa ttccagggca tcgccctgca cgaggagatc
     9421 cgctgacatg gctgccgtac acgatttcca gcatgacccg ggcttcgccg agcagcagga
     9481 cgccctgtcg acgctgctcg aaagcctgct gtcccccgaa ggcgtgcgcg ccgcgctcac
     9541 cgtctggtcg cgcgactacg cgcaccggcc cggggcgctg cgcgcctacg cgaactgcct
     9601 ggtgcgcgag ttcggcgtgc cgctcggcct gcgcgagatg caccgccagc tcgtgcagca
     9661 cctgctgctg ccggcaacgc gccgcggtcc ggtgctgctg gccgcggcgg cgcaaacccc
     9721 ggaccgggcg gtgccggcgc tgacgcgcgc cttcgtgctg ctggtcggtg cactgctcgc
     9781 gcgctgcggg cagcaggacc cgaaggccgc gacggcactg cgcagtgcgc tggtcggcgc
     9841 ggcgatgacg gcgaagctct cgcgcgccga tcgcgagctg ctggtcgcgt ggctggacgg
     9901 cagcggtccg gtgccggcgc gggcctacga cgaagccctg ctgcgcgaac tcgtgcatgc
     9961 ggcctgggcc gggctctgca cgctctgggg tccgccggtc gccgaccgcc agctcggcgc
    10021 ggccgtgcat gccgccgagg agcagtgcgc cgaggtccgg cgcctgctct gaacgccggc
    10081 gggcgcgagc cggcgcacgt ggcagccgtg gcgggtccgc gtatccttgc gcccccatgt
    10141 ccgatcccgc cctgagaatc ctgcgcaccg tcttcggcta cgacgccttc cgtggcgcgc
    10201 aggccgagat cgtcgcgcac gtcgccggcg gcggggatgc gctggtgctg atgccgaccg
    10261 gcggcggcaa gtcgctgtgc taccagatcc cgg
//
LOCUS       DAOQIE010000122        10268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3280_length_10268_cov_6.473906, whole genome shotgun sequence.
ACCESSION   DAOQIE010000122 DAOQIE010000000
VERSION     DAOQIE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10268
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_10268_cov_6.473906"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..663
                     /gene="dcd"
                     /locus_tag="PK144_09130"
     CDS             94..663
                     /gene="dcd"
                     /locus_tag="PK144_09130"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNF65929.1"
                     /translation="MSIKSDHWIRRMAREARMIEPFEPGQVRADADGARIVSYGTSSY
                     GYDVRCADEFKVFTNVHSTVVDPKAFDARSFVEVQGDRCIIPPNSFALARTVEYFRIP
                     RDVLVICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAVIYANEGVAQMLF
                     IGGDEVCGTSYADRGGKYQGQTGVTLPRT"
     gene            complement(707..1444)
                     /locus_tag="PK144_09135"
     CDS             complement(707..1444)
                     /locus_tag="PK144_09135"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNF65930.1"
                     /translation="MKTPCRPCRSLLAALGLALLAAGAPAAPQPPFRAQYVIGAAGFE
                     VGTAEVHGRAGDDGRLQVEARADGSGVVGLLFGELGTARFTVEQRPDGDRPLGFTIRV
                     DSPFDAEVALAYDWARGRLRVDYNGERREDTLAADTGDPFALMLALGDARRSGRPPPN
                     FWQTPDREGLRRYSVVRGTDETLATPLGPLAAERYDCIRVPRAGEDTLRIVVWLAPSL
                     DWLPVQGTRLRNGSEKARFRLAAWARE"
     gene            complement(1441..2172)
                     /locus_tag="PK144_09140"
     CDS             complement(1441..2172)
                     /locus_tag="PK144_09140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNF65931.1"
                     /translation="MRHCPALLRPAALLLGLALASPAPAAPLPALTARYALIAHGLTV
                     ARAELLSRPTPAGYELHARVEGGGLTGLFGELGRAELTGAAVPGRPDSRPLSFLVHSK
                     PPFTSATTLLYDWPAGRLHARHGESASDLPLEADLADPLAFFVELLEARRSGRPAPPR
                     LRVPYASGIVEYHLDSLGRETVPTALGARDAEKYELSRPDGGSGRVRITLWLSPADDW
                     LPVLAVRSRKHKERARAEIESLSRP"
     gene            complement(2233..2655)
                     /locus_tag="PK144_09145"
     CDS             complement(2233..2655)
                     /locus_tag="PK144_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNF65932.1"
                     /translation="MIHQEILIVRTPGRCTTDLSTQIGAVVGRSQVRTGIAHVFVQHT
                     SASLMLCENADPSVRRDLETFMARLAPDGAPWYAHDMEGPDDMPAHIRSVLTSNGLTL
                     PVGEGRLLLGTWQGVYLWEHRQSSHQRRLVVTVQGEQH"
     gene            complement(2652..3380)
                     /locus_tag="PK144_09150"
     CDS             complement(2652..3380)
                     /locus_tag="PK144_09150"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNF65933.1"
                     /translation="MPRRLIPGLLAAALAWPLTALAALPVPGFTARYSLALSGVPVGE
                     ATVTLDEPAPGRYRMRSEAAAKGQLAMLLPDRLSEEAEGTFSDDGPQPGHYRHERRGG
                     RERESVRIDFDPAAGEVRIEEAGSAPAKLALVPGMLDPLSLQLRAMWLLRQGRPPESF
                     TLVDDASPDTYRVESAGRETVKTGRGPVEAEVLRRGRPGDKRTATFWFAPKFGYVPVQ
                     IVQTRKGRETLRMTLIDVSGEAVR"
     gene            complement(3482..4141)
                     /gene="purN"
                     /locus_tag="PK144_09155"
     CDS             complement(3482..4141)
                     /gene="purN"
                     /locus_tag="PK144_09155"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNF65934.1"
                     /translation="MGSATKPGIVVLISGRGSNLQSILDRAASGELPVEVRAVISNRP
                     GVYGLQRARDAGVPALVLDHKDFADRTSFEAELIRQIDGFGPALVVLAGFMRILEPGF
                     CEHYRGRMLNIHPSLLPKYRGVHTHERALAAGETEHGVSIHFVTPELDGGPVFLQATV
                     PVLPGDDADTLAARVLVEEHKLYPLAIRWFAEGRVRLDGDTVLFDGAPLTAPKRLDDG
                     A"
     gene            complement(4141..5199)
                     /gene="purM"
                     /locus_tag="PK144_09160"
     CDS             complement(4141..5199)
                     /gene="purM"
                     /locus_tag="PK144_09160"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNF65935.1"
                     /translation="MPVSDQTPPIRLSYRDAGVDIDAGEALVERIKPHARRTARPGVL
                     GGLGGFGALFELPLDRYRQPVLVSGTDGVGTKLRLALDTGLHDTIGIDLVAMCANDIA
                     VAGAEPLFFLDYYATGKLDVDTAATVIQGIADGCALAGCALVGGETAEMPGMYEGADY
                     DLAGFCVGIVEKERIIDGSRVAPGDVLIGLASSGPHSNGYSLIRRILAVTGTSLDEPF
                     AGTTLGRALLAPTRIYVPAMLKLLEQIDVHSFAHITGGGLPDNLPRVLADGTRAVIDA
                     ASWQRPEIFRWLQQQGGVIESEMYRTFNCGIGMVVCVAPADVETTLASLAASGETAWV
                     MGRIEAAGDDAQPRVEIR"
     gene            5409..5972
                     /locus_tag="PK144_09165"
     CDS             5409..5972
                     /locus_tag="PK144_09165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNF65936.1"
                     /translation="MRARDIPNLITGLRLLLVPPILWLLWHDDHGAALLLFLIAGASD
                     ALDGALARGFHWQSRLGGILDPIADKSLLVGCFLVLGAGGELPLWLVALVLGRDLVIV
                     LGAAAWHWLIGALEARPSLISKLNTFMQLAVVLAVLLSHGATPLPDWLLRLLEWTMAL
                     TTLWSGIDYVWHWSRLARRHVRGEVAG"
     gene            5969..7021
                     /locus_tag="PK144_09170"
                     /pseudo
     CDS             5969..7021
                     /locus_tag="PK144_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            7249..7971
                     /locus_tag="PK144_09175"
     CDS             7249..7971
                     /locus_tag="PK144_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65937.1"
                     /translation="MKDQMPLKLAARERASFDNYVPGANLAVLEHLRRYTGSPGEPVL
                     YLHGPDGSGRTHLVQAVCSAAAARGQTTVRVGLTDTERLPLAALDGLENLAVVCIEDL
                     DAIAGQRTWEAALDQLCARLVARGGGLVMTGARVAVAAGFTHPGFAKRVAQAMDFALA
                     PLDAAGFAALLAARAGRLGLALPREVAALLLNHCADHPGRLIAALDALERALPQARGR
                     LGVTGVRALLRAEGLLAAPPSG"
     gene            8006..8554
                     /locus_tag="PK144_09180"
     CDS             8006..8554
                     /locus_tag="PK144_09180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF65938.1"
                     /translation="MPPESLLSADPLCPACPGVRLRIATPADLPLILGYIRALADYER
                     LSDQVVADEALLDAHLFGERPAAEVLLAELDGAPAGFALYFTSFSTFLGRPGIYLEDL
                     WVEPACRRRGVARTLLVALARLALARGCGRFEWSVLDWNEPAIRFYRALGAVGMDEWT
                     VQRVSGAALVRLAGGEIAADAG"
     gene            complement(8567..8884)
                     /locus_tag="PK144_09185"
     CDS             complement(8567..8884)
                     /locus_tag="PK144_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF65939.1"
                     /translation="MSSKGKPLDEAELEAWEATRDLAAELLQSVREMKAGQVHVVSSP
                     VIEARRKTGLSQSQFAALLGVSVRTLQGWEQGRKQPSGAARTLLAIASKNPKALLDVI
                     GEL"
     gene            complement(8868..9179)
                     /locus_tag="PK144_09190"
     CDS             complement(8868..9179)
                     /locus_tag="PK144_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF65940.1"
                     /translation="MFTVVETSLFQRQWPFYWSEEERGAFAAYIAGHPAAGDVVPESG
                     GIRKVRWSRSGSGKSGGVRVIYYTRIHEGMVVLLTLYAKSKTDNLTGQQLKEIRRVLE
                     G"
     gene            9449..9919
                     /locus_tag="PK144_09195"
     CDS             9449..9919
                     /locus_tag="PK144_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65941.1"
                     /translation="MTIPAETELTAEARKAISDAFDTIEKNLPFLISLTPDERKGRVL
                     MGDRNKPFVERTLEGAHIAPELLPRNIVIANVERQLALYRALSPVVARMTSLAEQIED
                     THSQVGSEVYTQALEMYRHFKAAGRTEGLDELIDNMGKRFNGQGRKKKTPPKDE"
BASE COUNT         1420 a         3562 c         3674 g         1612 t
ORIGIN      
        1 gcggtgatcg gtataaagaa gggcgcgcgg ttagaatcgc gcgcccttcc ttcatctgat
       61 ccggcccgcc tgcggccgag ccgaggtttg tccgtgagca tcaagtccga ccactggatc
      121 cggcgcatgg cccgtgaggc gcgcatgatc gagccgttcg agcccggtca ggtgcgcgcc
      181 gatgccgacg gtgcgcgcat cgtctcctac ggcacgtcga gctacggcta cgacgtgcgc
      241 tgcgccgacg aattcaaggt gttcaccaac gtgcattcga cggtcgtcga cccgaaggcc
      301 ttcgatgcgc gcagcttcgt cgaggtgcag ggcgatcgct gcatcatccc gccgaactcc
      361 ttcgcgctgg cgcgcacggt cgagtacttc cgcatcccgc gcgacgtgct ggtgatctgc
      421 ctcggcaaga gcacctacgc acgctgcggc atcatcgtca acgtcacgcc gctcgaaccg
      481 gagtgggaag gccacgtgac gctggagttc tcgaacacga cgccgctgcc ggcggtgatc
      541 tacgcgaacg agggcgtcgc gcagatgctg ttcatcggcg gcgacgaggt ctgcggcact
      601 tcgtatgccg atcgcggcgg caagtaccag ggccagaccg gcgtgacgct gccgcgcacc
      661 tgagcggaag gtgaagaggg gcggggagac gcttgcaggc ccggcctcac tcccgcgccc
      721 aggctgccag ccggaagcgc gccttctcgc tgccgttgcg aagccgcgtg ccctgcaccg
      781 gcagccagtc gagcgagggc gcgagccaca cgacgatgcg cagcgtgtcc tcgcctgcgc
      841 gcggcacgcg gatgcagtcg tagcgttcgg cggcgagcgg gccgagcggc gtggccagcg
      901 tctcgtcggt gccgcgcacg acgctgtagc ggcgcaggcc ctcgcgatcc ggcgtctgcc
      961 agaagttcgg cggcggccgg ccgctgcggc gggcgtcgcc gagcgcgagc atcagcgcga
     1021 acgggtcgcc ggtgtcggcc gcgagcgtgt cctcgcgccg ctcgccgttg taatcgacgc
     1081 gcagccgccc gcgtgcccag tcgtaggcga gcgcgacctc ggcatcgaag ggcgaatcga
     1141 cgcggatcgt gaagccgagc ggccggtcgc cgtccgggcg ctgctcgacc gtgaagcgtg
     1201 ccgtgccgag ttcgccgaac agcaagccga ccacaccgct gccgtcggcg cgcgcctcga
     1261 cctgcagccg gccgtcgtcg ccggcgcggc cgtggacctc ggcggtgccg acctcgaagc
     1321 cggcggcgcc gatcacgtac tgcgcgcgaa acggcggctg cggcgcggcc ggcgcgccgg
     1381 cggcgagcag cgcgagcccc agtgcggcga gcagcgatcg gcaggggcgg caaggcgtct
     1441 tcatggccgg ctcaggctct cgatctcggc gcgcgcgcgt tccttgtgct tgcggctgcg
     1501 cacggcgagc accggcagcc agtcatcggc cggtgacagc cacagcgtga tgcgcacgcg
     1561 cccggagccg ccgtccggac gcgacagctc gtacttctcg gcgtcgcgcg caccgagtgc
     1621 ggtcggcacg gtttcgcggc cgaggctgtc gaggtggtac tcgacgatgc cggaggcata
     1681 cggcacgcgc agccgcggcg gcgccgggcg gccgctgcgc cgggcttcga gcagctctac
     1741 gaagaaggcg agcggatcgg cgagatcggc ttcgagcggc aggtcgctgg cgctctcgcc
     1801 gtggcgggca tgcagcctgc cggccggcca gtcgtagagc agcgtcgtag ccgacgtgaa
     1861 cggcggcttc gaatgcacga ggaagctgag cggccggctg tccgggcggc cgggcaccgc
     1921 ggcgccggtc agctcggcgc ggccgagttc gccgaacagc ccggtcaggc cgccgccctc
     1981 gacgcgggcg tgcagctcgt agccggccgg tgtcggccgg ctcagcagct cggcgcgcgc
     2041 gacggtcagg ccgtgcgcga tcagcgcgta gcgggcggtc agcgccggca gcggtgcggc
     2101 cggcgccggg ctggcgaggg cgaggccgag cagcagtgcg gccggacgga gcagggcagg
     2161 gcagtggcgc atggcggtca gacgcgacgc agcgtttccg ggttcgcgcg cgcagtgcgt
     2221 ttcgtgcgcc gctcagtgct gttcgccctg cacggtgacc accagccggc gctggtggct
     2281 ggactggcgg tgttcccaca gatacacgcc ctgccaggtg ccgagcagca gccggccctc
     2341 gccgaccggc agcgtcaggc cattcgaggt cagcacgctg cggatgtgtg ccggcatgtc
     2401 gtccgggcct tccatgtcgt gggcgtacca cggcgcgcca tcgggcgcga gccgggccat
     2461 gaaggtttcg agatcgcggc gcaccgacgg gtcggcattc tcgcagagca tcagcgaggc
     2521 gctggtgtgc tgcacgaaca cgtgggcgat tccggtgcgt acctgactgc gcccgaccac
     2581 ggcgccgatc tgcgtgctga ggtcggtggt gcagcgcccc ggcgtgcgca cgatcaggat
     2641 ttcctggtga atcatcggac ggcttccccg ctcacgtcga tcaaggtcat gcgcagggtt
     2701 tcgcgcccct tgcgcgtctg cacgatctgc accggcacgt aaccgaactt cggcgcgaac
     2761 cagaaggtgg cggtgcgctt gtcgccgggc cggccgcggc gcagcacctc ggcctcgacc
     2821 gggccgcggc cggtcttcac ggtctcgcgc ccggcgctct cgacacggta ggtgtcgggg
     2881 ctggcgtcat cgaccagcgt gaagctttcc ggcggccggc cctgtcgcag cagccacatc
     2941 gcgcgcagct gcaggctcag cggatcgagc atgccgggga cgagcgcgag cttcgccggc
     3001 gccgagccgg cctcctcgat ccgcacctcg ccggccgccg gatcgaaatc gatgcgcacc
     3061 gattcgcgct cgcgtccgcc gcggcgctcg tggcggtagt ggccgggctg cggaccgtcg
     3121 tcgctgaacg tgccttcggc ttcctcgctc agccgatcgg gcagcagcat cgccagctgg
     3181 ccttttgcgg ctgcttcgga gcgcatgcgg tagcgccccg gtgccggctc atcgagcgtg
     3241 acggtggcct cgccgaccgg cacgccggac agcgccagcg aatagcgggc cgtgaagccg
     3301 ggcaccggca gcgcggccag ggcggtcagc ggccaggcca gcgcggcggc gagcaggcca
     3361 gggatcaggc ggcgcggcat gcgggcactc cggacgggaa ggcggatatc cgccagccta
     3421 cacccgcggc gcgcgtgcgg cctgcggcat ccggcgtagc cgctgcggga aaacccgcag
     3481 ctcaggcgcc gtcgtccagc cgcttcggtg ccgtcagcgg cgcgccgtcg aacagcacgg
     3541 tatcgccatc gaggcgcacg cggccctcgg cgaaccagcg gatggccagc ggatacagct
     3601 tgtgttcctc gaccagcacg cgcgcggcca gggtgtcggc atcgtcgccg ggcagcaccg
     3661 gcaccgtcgc ctgcaggaac accgggccgc cgtcgagctc gggcgtgacg aagtggatgc
     3721 tgacgccgtg ctcggtctcg ccggcggcga gcgcgcgttc gtgcgtgtgc acgccgcggt
     3781 acttcggcag cagcgacgga tggatgttga gcatgcgtcc gcggtagtgc tcgcagaagc
     3841 ccggctccag gatgcgcatg aagccggcaa gcacgacgag tgccggcccg aagccgtcga
     3901 tctgccggat cagctcggcc tcgaagctcg tgcggtccgc gaagtccttg tgatcgagca
     3961 ccagcgccgg cacgccggca tcgcgcgcgc gctgcagccc gtacaccccg ggacggttgc
     4021 tgatcaccgc gcgcacctcg accggcagtt caccgctcgc ggcacggtcg aggatgctct
     4081 gcaggttgct gccgcggccc gagatcagca cgacaatgcc gggtttcgtg gccgagccca
     4141 tcagcggatc tccacgcgcg gctgcgcgtc gtcaccggcg gcctcgatgc ggcccatgac
     4201 ccaggccgtc tcgccgctgg cggcgaggct cgcgagcgtg gtttcgacat cggccggcgc
     4261 cacgcacacg accatgccga tgccgcagtt gaaggtccgg tacatctcgg attcgatcac
     4321 accgccctgc tgctgcagcc agcggaagat ctccggccgc tgccagctcg ccgcgtcgat
     4381 gacggcgcgc gtgccgtcgg cgagcacgcg cggcaggttg tcgggcaggc cgccgccggt
     4441 gatgtgcgcg aagctgtgca cgtcgatctg ctccagcagc ttcagcatcg ccggcacgta
     4501 gatgcgcgtc ggcgcgagca gcgcgcgccc gagcgtggtg ccggcaaagg gctcatcgag
     4561 gctggtgccg gtgacggcga ggatgcgccg gatcagcgaa tagccgttcg agtgcgggcc
     4621 gctcgacgcc agaccgatca gcacgtcgcc gggcgcgacc cggctgccgt cgatgatgcg
     4681 ctccttctcg acgatgccga cgcagaagcc ggcgaggtcg taatcggcgc cctcgtacat
     4741 gccgggcatc tcggcggtct cgccgccgac cagcgcgcag ccggcgagcg cgcagccatc
     4801 ggcgatgccc tggatgacgg tcgcggcggt gtcgacatcg agcttgccgg tggcgtagta
     4861 atcgaggaag aacagcggct cggcgccggc aaccgcgatg tcgttggcgc acatcgccac
     4921 caggtcgatg ccgatggtgt cgtgcaatcc ggtatcgagc gcgaggcgca gcttggtgcc
     4981 gacgccgtcg gtgccggaga ccagcaccgg ctggcggtag cgatccagcg gcagctcgaa
     5041 cagcgcgccg aagccgccga ggccgccgag cacgccgggg cgggccgtgc gccgggcgtg
     5101 cggcttgatg cgttcgacga gcgcctcgcc ggcgtcgata tcgacgcccg cgtcgcgata
     5161 gctgaggcgg atgggcggag tctggtcgct cacggggatc acctgggtcg ggcggcggac
     5221 ggaggggaac aggcagcggg ccgcagccgg cgtgcgaaac cgggcattct acacatgccc
     5281 ggcggcaaga ccggagccgg ggccgtgtgg cagcgtccga cgctcatttg acagtgcgcg
     5341 aggcggctca ccagactgcc cggcactccc gaccgccgcg tttcccgccc gctccctgca
     5401 tgccctccct gcgcgcccgc gacatcccga acctgatcac cggcctgcgc ctgctgctgg
     5461 tgccgccgat cctgtggctg ctgtggcacg acgatcacgg tgccgcgctg ctgctgttcc
     5521 tgatcgccgg cgcttccgat gccctcgacg gtgcgctcgc gcgcggtttt cactggcaga
     5581 gccgcctcgg cggcatcctc gacccgatcg ccgacaagtc gctgctggtc ggctgctttc
     5641 tggtgctcgg tgccggcggc gagctgccgc tgtggctggt ggcgctggtg ctcggccgcg
     5701 atctggtcat cgtgctcgga gccgccgcct ggcactggct gatcggcgcg ctcgaagccc
     5761 ggccctcgct gatttccaag ctcaacacct tcatgcagct cgccgtcgtg ctcgcggtgc
     5821 tgctcagtca cggcgccacg ccgctgccgg actggctgct gcgcctgctc gaatggacga
     5881 tggcgctgac cacgctctgg agcggcatcg actacgtctg gcactggagc cggctcgcgc
     5941 gccggcacgt gcgcggcgag gtggccggat gaacgccgac gagcggcgca cctgggcgct
     6001 cgggctggtg gggctggtgg tcgccggcta cggcctgtgg ctgctgtcgc cggtgctcgc
     6061 gcccttcctg ttctcggccg tgctcgccta tctcggtgac ccgctcgccg accggctcga
     6121 acggctcggg ctgtcgcgca cgctgtcggt ggtgacggtg ttcgtcagca tgttcgcgct
     6181 gatcctggtg ctgctgctgt tcatcgtgcc gatgctcgaa cgccaggcca cgcagctctt
     6241 cgaccaggcg ccggcggcga tcgactggta caacgtcgag gtgcgcggct ggctgcgcca
     6301 gcagttcaac ctgccgctgc cgcagctgcg cttcgacgcg atcaagaagg aagtcgtcac
     6361 cgcgctgcag tcggccggct ccgacagcca cgcgctgatc gccaccatca gccagtcctc
     6421 ggcggtgctg gtcggctgga cggtgacgct gctgctggtg ccggtggtga ccttctacct
     6481 gctgcgcgac tgggacctgc tggtcgagga catccgccac ctgctgccgc gctcggtcga
     6541 gccgcaggtc tcgaagctcg cgcgcgaatc cgatgccgtg ctcggcgcct tcctgcgcgg
     6601 gcagttcgcg gtgatgctcg cgctcgccgt gatctacggc ggcggcctga cgctggtcgg
     6661 gctcgactac ggcctgctga tcggcctgat cgccgggctg gtgagcttcg tgccgtactt
     6721 cggcttcttc ttcggcatcg tgctcgcggg gctggcggcg atggtgcagt tccacgcgcc
     6781 gctgccgctg cttgcgacct tcggcgtgtt cgcgttcggg cagctgctcg aatccttcat
     6841 cctgacgccg cgcttcgtcg gcggcagcat cggcctgcac ccggtggcgg tgatcttcgc
     6901 gctgatggcc ggcgagcacc tgttcggctt cttcggcatc ctgctggcgc tgccggtggc
     6961 ctcggtggtc atggtgctgc tgcgccacac gcacgcgcac tacgtgcaga gcgaactcta
     7021 cgagaccccg ccggcgccgc atttcgagcc tgatgagatc gtcgagtccg cggtcacgca
     7081 cgagaccatc cgcgcgctcg atgccgatgc tgccggggac gaggccgggg cggcggcgga
     7141 cgatgacgaa gtgctggcgc gtgtcaccac cgtcgagcgc accgaaaccg tcaccaccga
     7201 aaccatcgag gcgctgcgcc cggccggcgc accgccggag cgcccggcat gaaggaccag
     7261 atgccgctga agctcgccgc ccgcgagcgc gcgagcttcg acaactacgt gcccggcgcc
     7321 aacctcgccg tgctcgaaca cctgcgccgc tacaccggca gccccggcga gccggtgctg
     7381 tacctgcacg gccccgacgg cagcgggcgc acgcacctgg tgcaggcggt gtgcagcgcc
     7441 gccgccgccc gcggccagac gacggtgcgc gtcggcctca ccgacaccga acgcctgccg
     7501 ctcgccgcgc tcgacggcct cgaaaacctc gccgtggtct gcatcgagga cctcgacgcc
     7561 atcgccggcc agcgcacctg ggaggccgcg ctcgatcagc tctgcgcgcg gctggtggcg
     7621 cgcggcggcg ggctggtgat gaccggtgcc cgcgtcgcgg tcgcggccgg cttcacgcat
     7681 ccgggcttcg cgaagcgcgt cgcgcaggcg atggacttcg cgctcgcccc gctcgatgcc
     7741 gccggcttcg ccgcgctgct cgccgcccgc gccggccggc tcgggctggc gctgccgcgc
     7801 gaggtcgccg cgctgctgct caatcactgc gccgatcacc ccggccggct gatcgccgcg
     7861 ctcgatgcgc tggagcgcgc gctgccgcag gcccgcggcc ggctcggcgt caccggcgtg
     7921 cgtgcgctgc tgcgcgccga gggcctgctc gccgcgccgc cgtccggctg aaccctccgc
     7981 cccgccgtcc gtggagcccc tgcccgtgcc gcccgaatcc ctgctgtccg ccgatccgct
     8041 gtgcccggcc tgtcccggcg tgcggctgcg catcgccacg cctgccgacc tgccgctgat
     8101 cctcggctac atccgcgcgc tcgccgacta cgagcgtctg tccgatcagg tggtcgccga
     8161 cgaggcgctg ctcgatgcgc atctgttcgg cgaacgcccg gccgccgagg tgctgctcgc
     8221 cgaactcgac ggcgccccgg ccggcttcgc gctgtacttc accagcttct cgaccttcct
     8281 cggccgcccc ggcatttacc tcgaagatct ctgggtcgaa ccggcctgcc gccgccgcgg
     8341 cgtggcccgc acgctgctgg ttgcactcgc gcggctggcg ctggcgcgcg gctgcgggcg
     8401 cttcgagtgg tcggtgctcg actggaacga accggcgatc cgcttctacc gcgcgctcgg
     8461 cgccgtcggc atggacgaat ggacggtgca gcgcgtcagc ggcgcggcac tggtgcggct
     8521 ggcgggcggg gagattgcgg cggatgcggg ctgagcgttt gccggcttac agttcgccga
     8581 tcacatccag cagcgccttg gggtttttgc tggcaatcgc cagcaaggtg cgggctgcac
     8641 cgctcggttg cttgcgaccc tgttcccagc cctgcagggt gcgaacggac actccaagca
     8701 atgcagcgaa ctgcgattgc gacaagccgg ttttcctgcg cgcttcgatg accggcgaac
     8761 tcacgacatg cacctgtccc gccttcattt cacgcacgga ctgcagcagt tctgccgcaa
     8821 gatcccgcgt ggcttcccag gcttccagtt ctgcttcgtc gagtggctta cccttcgagg
     8881 acacggcgga tctccttcag ttgctggccg gtcaggttgt cggtctttga tttcgcatac
     8941 aaggtgagca gtacgaccat gccctcatgg atgcgggtgt agtaaatgac ccgcaccccg
     9001 ccggacttgc ccgagcccga gcgactccag cgtactttgc gtatgccgcc ggattcgggc
     9061 acgacatcgc cggcagcagg atgcccggcg atataggcgg cgaaagcgcc gcgttcctct
     9121 tccgaccagt aaaacggcca ctgccgctga aagaggctgg tttcgacgac ggtgaacatg
     9181 ccgcgaccct acgcctcggg cgcagggtcg tctacttgcg caaacgatgg gccaccgcat
     9241 cagtccattc tgtccggcag tttcgggacc ggctttccgg ctttcccgcc tcgcctgaac
     9301 aaatcgtcat ccgcaatacg acatctgcct gatggttccg ccggcggatt cgggggtatt
     9361 cgcgtggcag gaccgcgacg ggaagcgcag gggcgcgacg gcggtgccgg cgcggcgtgg
     9421 gcgccaacca cgggaggagg agatgatgat gacgattccc gctgaaaccg aactgacggc
     9481 cgaggcgcgc aaggcgatca gcgatgcctt cgacaccatc gagaagaacc tgccgttcct
     9541 gatctcgctg acgcccgacg agcgcaaggg ccgggtgttg atgggggacc gcaacaagcc
     9601 gttcgtcgag cgcacgctgg agggcgcgca cattgcgccg gagctgctgc cgcgcaacat
     9661 cgtgattgcc aatgtcgagc ggcagctggc gctgtaccgg gcgctgtcgc cggtggtggc
     9721 gcgcatgacc agtctggccg agcagatcga ggatacgcac agccaggtgg ggtcggaggt
     9781 ctacacgcag gcgctggaga tgtaccgcca cttcaaggcg gcgggccgca ccgagggtct
     9841 ggacgagctg atcgacaaca tgggcaagcg cttcaacggt cagggccgca agaagaagac
     9901 gccaccgaag gacgagtgat ccggtctgat ccctgaaccc cgctgcctgt gccggcccgc
     9961 cactgcgcga gccggcacgg gcagcggggt ttgttttgtc cggtgccggt ggtgtgcgca
    10021 ggcgagcggc tggccgtggt cgggcagcgg cttgcctttg gaggggagct gactaggtat
    10081 tttcggaagt cagcataggg tgattgctaa catggtttcc gttgaagcga aactggttat
    10141 gtttgaagcg aaaccagtta tgtccgcagc ggaactggtt atgtctgaag tgaaaccggg
    10201 tttcggtctg aggggaactg gttatgtccg gagtgaaacc gggtttcgtt tcaagtgaaa
    10261 ccggttat
//
LOCUS       DAOQIE010000123        10262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3282_length_10262_cov_7.797982, whole genome shotgun sequence.
ACCESSION   DAOQIE010000123 DAOQIE010000000
VERSION     DAOQIE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3282_length_10262_cov_7.797982"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(993..1340)
                     /locus_tag="PK144_09200"
     CDS             complement(993..1340)
                     /locus_tag="PK144_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65942.1"
                     /translation="MARRRQAQGSQLRRRRYDSRHVYRRVRPMNAPYQPPTDILDVLR
                     DIEFEDALTAEDARYVDTREARGSQKTLRMLSRMFGLQLPDGPFRWPLTKHVLDAAQN
                     AGSHENTAVAFSH"
     gene            1417..2169
                     /locus_tag="PK144_09205"
     CDS             1417..2169
                     /locus_tag="PK144_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF65943.1"
                     /translation="MSFDALHLFVLLAAAFVAGALNAVAGGGSFLTLPALVWAGVAPV
                     AANATGTAALLPGYVASAWGYREDLRAHAGAGLLALICTGAAGGALGAALLLATPDKA
                     FRALVPWLLLAATALFAAGPWLLARLKRDGEAGVAASLATVFAVSVYGGYFNGGLGIL
                     LLAAFGLLGQRDLNRMNGLKNLLSAVLTLIAVSLYALGGAVQWAPALAMALAATAGGY
                     AGARIGRRIPAKWLRAGIVATGTVMAGLFFRG"
     gene            2317..3486
                     /gene="argE"
                     /locus_tag="PK144_09210"
     CDS             2317..3486
                     /gene="argE"
                     /locus_tag="PK144_09210"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNF65944.1"
                     /translation="MPVAAPDLLSMIRGLVALPSVSAVQPTWDQGNLAVVERLADWLE
                     ALGFRCELLALDDPRKANLVATLGSGPGGLVLAGHTDTVPYDSSGWRHDPFGGIEADG
                     RIYGLGTADMKSFLALAAEAARGLRAQDLRRPLVILATADEESSMDGARALVAAGRPL
                     GRYAVIGEPTGLKPIRLHKGVMMEAIRVQGRAGHASDPSLGNSALEGMHRVIGELLAM
                     RAGWATRYRNPQFAVEVPTLNLGHIHGGDNPNRICAHCELHIDIRPLPGMCLHALREE
                     IDAHLARALEGTGLGWSSEALIEGIEPFETPADSRLVAAAEQLTGHTAGSVAFGTEGP
                     YLNKLGMDTIILGPGDIAQAHQPDEYLALDRIPPTLELLRALIRRFCIDVPDPAA"
     gene            3510..4835
                     /gene="argA"
                     /locus_tag="PK144_09215"
     CDS             3510..4835
                     /gene="argA"
                     /locus_tag="PK144_09215"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNF65945.1"
                     /translation="MNDPKKTLVQGLRQAVPYINALEHKTLVILFGGEAVVDVERFAS
                     LIHDIALLDSLGLKLVLVHGARPQVEAELAEMGHEPRFADGVRITDTVALDAVKEAVG
                     ATRILIEGLLSMGLANSPMHGARLRVGSGNAVVARPLGVREGVDFQYTGEVRRVDGEL
                     IRAWHQADTIVLLSPLGYSPTGEVFNVSAREVAVMAARTLGAEKLVMLTEEGGIVDHD
                     GRLLSDLSVPQAERLLAADTAMLDADQAEALDNAIAACRGGVRRVHLVPRRLDGALLL
                     ELFTRDGVGTMVFADQYEGARPATIDDVGGLIELIRPLEEDGTLVRRSRERLENEIDR
                     FTLLERDGAIIGCAALYAFPDEAIGELACVAVHADYRRGGRADALLGAIEARARGMGL
                     TRLFVLTTRTAHWFRERGFEPADLDALPVGKREMYNFQRRSKVFVKPLL"
     gene            5147..5461
                     /locus_tag="PK144_09220"
     CDS             5147..5461
                     /locus_tag="PK144_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF65946.1"
                     /translation="MKAIHGIAGALLLGAIVAAPAQADGLALAQKNNCLACHAVDQKL
                     VGPSYKEVAAKYKGDATAPDKLAAKVKAGGAGSFGQIPMPPNPQVSDADMKEIIAWIL
                     AL"
     gene            5676..5933
                     /locus_tag="PK144_09225"
     CDS             5676..5933
                     /locus_tag="PK144_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65947.1"
                     /translation="MVPALCQDCLRVWEFVDWETAMQATCTCGGQICSCPKCYRRIQS
                     LHARRWEAAQLGLPNGVVLLGWNAYDGAQFTDLRREPLKHD"
     gene            6318..9236
                     /locus_tag="PK144_09230"
     CDS             6318..9236
                     /locus_tag="PK144_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNF65948.1"
                     /translation="MTHPAFEHLRSRAVPALKLELQEYRHRATGARHVHLASDDPHKA
                     FLVAFLTVPQDSTGVAHILEHTALCGSRRYPVRDPFFMMTRRSLNTFMNAFTGADWTA
                     YPFASLNRKDFENLLDVYLDAAFFPLLDPLDFAQEGHRVEFADDSADAPLVFKGVVYN
                     EMKGAMSSPVQALAQRLQSALFPTTTYHHNSGGEPGVIPDLTHERLVAFHGTHYHPSN
                     AVFMSYGDLPAAELQQHFEERALSAFTARPLDLAIPDERRYDAPVAVEAPYAADAEAL
                     ADGTHVVLGWLLGRTTDPLETARARVLSDVLLDNSSSPLRHALETSELGAAPSPLCGF
                     DDSTRETTFTCGLEGCAPDTAAAVEQLVLETITRVAEDGVAPEVVESVLHQLELQSRE
                     ISGDGYPYGLKLMLDALTPLMHGGDPLDGLDIDPVLAELRREAAEPGFIPQLARRLLL
                     DNPHRVRLSLKPDPELAARQAAAEIERLAALRAQLDAEAQQQVRAQAAALAARQARHD
                     DPALLPKVTLADVPAELAIATGETRQLNGVETAWYAQPTNGLVYQQWVIDLPALDPGL
                     AGLVPLYALTLSEVGSAGRDYQETQALQAAVTGGLSARCLVRAAVDDPQQLRAVLVLA
                     GKALARNAGALTQLLRDTLLEPRFDELDRLAELVAQYRAQRDEEVVDHGHALAMGAAS
                     EALSPAGALISQWGGLTAIERLRELDDALSADDDEADAALATYAQRLAALHARVLCAP
                     RRLLVVAEAGRHGELAELIATHGAGPAAAGTAVTLAPALPDSPPVQAWTLATQVSFCA
                     RAYPAVAAGHPDAPALVVLGDFLRNGFLHRAIRERGGAYGAGAGLHADSGSFRFFSYR
                     DPRLAETLADFDASIDWLLSTRHEAQPLEEAILGVVAAIDRPGSPAGEAINAWFSELF
                     GRTPEQRRRFRARVLAVTIEDLQRVARTWLRPELARTAVITSAAALAASGLNAEVRTL
                     "
     gene            complement(9405..>10262)
                     /locus_tag="PK144_09235"
     CDS             complement(9405..>10262)
                     /locus_tag="PK144_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF65949.1"
                     /translation="EDCVNAVGVDVNTASVPLLTRVSGLNATLARNIVAWRDANGAFR
                     NRAQLLKVPRLGDKTFEQAAGFLRIAHGDNPLDRSAVHPEAYPVVERILKRAGRDIGG
                     VLGDTGFLRGLRPEQYTDGRFGVPTVRDIIAELEKPGRDPRPEFRTAAFREGVEDLKD
                     LEADMVLEGVVTNVTNFGAFVDIGVHQDGLVHVSCLSEKFVKDPHQVVRAGDVVRVKV
                     LEVDVKRRRIALTMRLADQARDHQPGAGKPAGTARSGRPPQAQPPARAQKPAGGGAFA
                     DAFARARRK"
BASE COUNT         1499 a         3643 c         3520 g         1600 t
ORIGIN      
        1 gctctgccgc gccgcggcgg atgccgggca tcgccggttc gacgcaaccg ccaccccgcc
       61 agcctcgcag cccgcacatc cgcccatcga gccccccgcc ctgcagcccg actccggaag
      121 tttgcggccg gacagcgcgg gcttgtgttc aatgccagtg caataacgct ccaactttgt
      181 gcgcgacatt atctggcgaa cccatttcca ttcgaatctc gcacatttcc aatggaaaaa
      241 tgctcgatca caatggaaat ggatacattt ccgttgcaat ctcgcaacat tgccatggaa
      301 acacgcgaat ctccattgca acctcgtaca tttccatccc gtttttgtgc agcgaagctt
      361 cagcccgcca catttccgtg catcgcgagc acatttccag tggaaagatg cgaagtctga
      421 atggaaatgt gcatggttgg aatggaaacg ggcgtgcttc gatgccgatg gcgcgcggtt
      481 tgacggcaaa gcggcaggat gaatggcatc gggcgtgggt tggaggatgg ggggcgggcg
      541 gttgcggtcg tgcccggcgc ggtatgaaca atctgagcat tcgtaggatg ggctgaacgc
      601 agtgaagccc atcattcgcg taccgttcgc ggaagatggg cttccttcgt cagcccatcc
      661 tacgggcttg gcatggcaag ccgggcgcca atcctccagc cagcctacgg cgatgcatcc
      721 ggctcgaagc cgtgcgcgcg catgattgcc gcgatttcct cggcttccgg aatccgcatc
      781 gacaacgccg ccacacgtgc ctgacgcagc agggcttcgg cccgggggcg gtcttgcgga
      841 tgcgcagggc tcgtcgtaat cccccaggtc ctgcagcaat ccggccagtt cgtcgagcag
      901 cacgacgcga atcccgtcgt ccatagcctt gcgggtcttt cagccagtca cgcagtccgg
      961 cgatgcgggc acgttccggt agcaggcgtg ggtcaatgac tgaatgccac tgcggtattt
     1021 tcgtgggaac cggcgttttg agcggcgtcg agaacatgct tggtcagcgg ccagcgaaac
     1081 ggcccgtccg gcagttgcag gccgaacatg cgcgacagca tgcgcagcgt cttctgactg
     1141 ccgcgcgcct cgcgcgtatc gacgtaacgc gcatcttcgg cagtcagggc atcctcgaac
     1201 tcgatgtccc gcagcacatc gaggatgtcg gtcggtggct ggtacggagc attcatcggt
     1261 cgaacccggc ggtaaacgtg gcggctatcg tagcgcctgc gccgcagctg cgatccctgg
     1321 gcctgccgcc ggcgcgccac gtcccggccc cgcggccgtg ccgcctgcag cgcagggccc
     1381 tagactcgcg tccccttccc tgcccgagtc ctgcccgtga gcttcgatgc cctgcacctg
     1441 ttcgtcctgc tcgccgcggc cttcgtcgcc ggggcgctga atgccgtggc cgggggcggg
     1501 agtttcctga cgctgccggc gctggtctgg gccggcgtgg cgccggtggc ggccaatgcc
     1561 accggcaccg cggcgctgct gccggggtac gtggccagtg cctggggcta tcgcgaggat
     1621 ctgcgcgcgc atgccggtgc ggggctgctg gcgctgatct gcaccggcgc ggctggcggt
     1681 gcgctcgggg cggcgctgct gctggcgacg ccggacaagg cgttccgggc gctggtgccg
     1741 tggctgctgc tggcggcaac agccttgttc gcggccgggc cgtggctgct cgcgcgcctg
     1801 aagcgcgacg gcgaggccgg ggtggcggcg agtctggcga ccgtgtttgc ggtgtcggtg
     1861 tacggcggtt acttcaacgg cgggctgggc atcctgctgc tcgcggcctt cgggctgctc
     1921 gggcagcgcg atctcaaccg catgaacggg ctgaagaacc tgctctcggc ggtgctgaca
     1981 ctgatcgccg tgagcctcta tgcgctcggc ggcgccgtgc agtgggcgcc ggcgctggcg
     2041 atggcgctcg cggcgaccgc cggcggctat gccggggcac gcatcggccg gcgcattccg
     2101 gcgaagtggc tgcgggcggg catcgtcgcg accgggacgg tgatggcggg gctgttcttc
     2161 cgcggctgag cggaccggag ccgcgcgagg gcggtcgctt cgggccagcc ggggctgtgc
     2221 agcggcgatg ccgggcatcg cgggctttgc ctcaacggat gcgcgatccg ccggtctaga
     2281 atccccgtcc ctgccgtcac cccgggagtc ccgaacatgc ctgttgccgc tcccgatctc
     2341 ctcagcatga tccgcgggct cgtggcgctg ccttcggtca gtgccgtgca gccgacctgg
     2401 gatcagggca atctcgccgt cgtcgaacgg ctggccgact ggctcgaagc gctcggcttc
     2461 cgctgcgagc tgctcgcgct cgacgatccg cgcaaggcca atctcgtcgc cacgctcggc
     2521 agcggcccgg gcgggctggt gctcgccgga cacaccgaca ccgtgcccta cgattcctcg
     2581 ggctggcgcc acgacccgtt cggcggcatc gaggccgacg ggcgcatcta cggactcggc
     2641 accgccgaca tgaaatcctt cctcgcgctc gcggccgagg cggcgcgcgg gctgcgtgcg
     2701 caggacctgc gccggccgct ggtgatcctc gcgacggccg acgaggaatc gagcatggac
     2761 ggggcgcgtg cgctggtcgc cgccggccgg ccgctcgggc gctacgcggt gatcggcgag
     2821 ccgaccgggc tgaagccgat ccgcctgcac aagggcgtga tgatggaggc gatccgcgtg
     2881 cagggccgcg ccggtcatgc ctcggacccg agcctcggca acagcgcgct cgaaggcatg
     2941 caccgcgtga tcggcgagct gctggcgatg cgcgccggct gggcgacgcg ctaccgcaat
     3001 ccgcagttcg cggtcgaggt gccgacgctg aacctcggcc acatccacgg cggcgacaat
     3061 ccgaaccgca tctgcgcgca ctgcgagctg cacatcgaca tccggccgct gccgggcatg
     3121 tgtctgcacg cgctgcgcga ggagatcgac gcgcatcttg cgcgtgcgct cgaaggcacc
     3181 ggcctcggct ggagcagcga ggcgctgatc gagggcatcg aaccgttcga gacaccggcc
     3241 gattcgcggc tggtggcggc ggccgagcag ctcaccggcc acaccgccgg ttcggtggcc
     3301 ttcggcaccg aaggtccgta cctgaacaag ctcggcatgg acacgatcat cctcggcccc
     3361 ggcgacatcg cccaggccca ccagcccgac gagtatctgg cgctcgatcg cattccgccg
     3421 acgctggaac tgttgcgcgc gctgatccgt cgcttctgca tcgacgtgcc cgatcccgct
     3481 gcatgacgcc ctccctgacc gaacgcgcga tgaacgatcc caagaaaacg ctggtgcagg
     3541 gcctgcgcca ggccgtgccg tacatcaatg ccctcgaaca caagacgctg gtgatcctgt
     3601 tcggcggcga ggccgtcgtc gatgtcgagc gcttcgcctc gctgatccac gacatcgcgc
     3661 tgctcgactc gctcggcctg aagctcgtgc tcgtgcacgg cgcccggccg caggtcgagg
     3721 ccgaactcgc cgagatgggc cacgaaccgc gcttcgccga tggcgtgcgc atcaccgaca
     3781 cggtcgcgct cgatgcggtc aaggaggcgg tcggtgcgac gcgcatcctg atcgagggcc
     3841 tgctgtcgat ggggctcgcc aattcaccga tgcacggcgc gcggctgcgc gtcggctccg
     3901 gcaacgccgt cgtcgcccgg ccgctcggcg tgcgcgaggg cgtggatttc cagtacaccg
     3961 gcgaggtgcg ccgcgtcgac ggcgaactga tccgcgcctg gcatcaggcc gacaccatcg
     4021 tgctgctgtc gccgctcggc tattcgccga ccggcgaggt gttcaacgtc tcggcgcgcg
     4081 aggtcgcggt gatggcggcg cgcacgctcg gggccgagaa gctggtgatg ctgaccgagg
     4141 aaggcggcat cgtcgatcac gacggccggc tgctgtcgga cctgagcgtg ccgcaggccg
     4201 agcgcctgct cgcggccgat acggcgatgc tcgacgccga tcaggccgaa gccctcgaca
     4261 acgccatcgc cgcctgccgc ggcggcgtgc gccgcgtgca tctggtgccg cgcaggctcg
     4321 acggcgcgct gctgctggag ctgttcacgc gcgatggcgt cggcacgatg gtgttcgccg
     4381 accagtacga gggcgcgcgc ccggcaacga tcgacgatgt cggcggcctg atcgagctga
     4441 tccgcccgct cgaagaggac ggcacgctgg tgcgccgctc gcgcgagcgg ctggagaacg
     4501 agatcgaccg cttcacgctg ctcgaacgcg acggcgcgat catcggctgc gcggcgctgt
     4561 acgccttccc ggacgaggcg atcggcgaac tcgcctgcgt ggccgtgcac gccgattacc
     4621 gccgcggcgg ccgcgccgat gcgctgctcg gggcgatcga ggcgcgcgcg cgcggcatgg
     4681 gtctcacgcg cctgttcgtg ctgacgacgc gcaccgcgca ctggtttcgc gaacgcggct
     4741 tcgaaccggc cgatctcgat gcgctgccgg tcgggaagcg cgagatgtac aacttccagc
     4801 gccgctcgaa ggtgttcgtg aagccgctgc tgtgagccgg tgcgaggttt tttcgtctgt
     4861 cttcgcatgt cattgagcaa tcgcaacgcc gcagcccgca gcaaccggca acatccgggc
     4921 gcgcggtttc ggatgacggc aatccggccg gcagtcagcg gcataccacg caatagcgac
     4981 tgcggcggct cgacggagcc ggatttctgg tcaaaactgc gcgccggcga tggcggcccc
     5041 ggtgtgccac cccgccccgg acgcccgccc ggctgtcgcc cgccggcaca gcccggcatg
     5101 gaccgccggt actgaacccc tgtaccccct tcgaggagag agccacatga aagccattca
     5161 cggcatcgcg ggcgccctgc tgctcggcgc gatcgtcgcc gctccggcgc aggccgacgg
     5221 cctcgcgctg gcgcagaaga acaactgcct cgcctgccac gccgtcgacc agaagctggt
     5281 cggcccgtcc tacaaggaag tcgccgcgaa gtacaagggt gacgccacgg cccccgacaa
     5341 gctggccgcc aaggtcaagg ccggcggtgc cggttccttc ggccagatcc cgatgccgcc
     5401 gaatccgcag gtgtcggacg ccgacatgaa ggaaatcatt gcctggatcc tggcgctgta
     5461 agctccgcgc ccgcgcagaa actaaaacgg ccgcccccgg gcggccgttt tcattggcgg
     5521 gcaccgcagc cgcacgccct ccggaacagg cccgattctt gcgcattctc cggtgatgcc
     5581 gcctgcatgc gacaaaaccg acacggcccg tgtgcgcgac agcttccgtc aagaccttga
     5641 tcgatcggga tcaacagccc ggaggacagt gctacatggt ccctgccctt tgtcaggatt
     5701 gtctgcgcgt gtgggaattc gtcgactggg aaaccgcgat gcaggccacg tgtacctgcg
     5761 gcggccagat ctgttcctgc ccgaagtgct accgccgcat ccagtccctg cacgctaggc
     5821 gctgggaggc cgcgcaactc ggcctgccga acggcgtcgt gctgctcggc tggaacgcct
     5881 acgacggcgc gcagttcacc gacctgcgac gcgaaccgct gaagcacgac tgatccgcag
     5941 gacacggccg ggcggctgcg ccgcacgcgg ccggtcgcag cgccagtgcc cggcggccgt
     6001 gacaggaacg gcgcgctgcc ccgcgcccgc agcctgcacc ggtgcagccg tgcatccgct
     6061 gcgctgcgca cggtctgctg ctgcgcgtca tgcattgctg ccatgccacg gaacgatgcc
     6121 tgtcgtgttc cgggattgct gaatacatga tggtataagt aatgatctga acaggttacc
     6181 acccacctcc gggccggcgt tcaggctgca tcctgcggca ccgtcagcgg ggcttccggt
     6241 cgaaagctga tacgcttttt ccatccggcc cgagggggcc ggcccgtatc gctccgactg
     6301 ccttcccgag acgcccgatg acgcaccccg ccttcgaaca cctgcgcagc cgtgccgtgc
     6361 cggcgctgaa gctggaactg caggagtacc gccaccgcgc caccggcgcc cggcacgtgc
     6421 atctggcgag cgacgatccg cacaaggcct ttctggtcgc gttcctgacc gtgccgcagg
     6481 actcgaccgg cgtcgcgcac atcctcgaac acacggcgct ctgcggcagc cgccgctacc
     6541 cggtgcgcga tccgttcttc atgatgacgc gccgctcgct gaacaccttc atgaacgcct
     6601 tcaccggcgc cgactggacg gcgtatccgt tcgcgagcct caaccgcaag gacttcgaga
     6661 acctgctcga tgtctacctc gacgccgcct tcttcccgct gctcgatccg ctcgacttcg
     6721 cgcaggaagg ccatcgcgtc gagttcgccg atgattccgc cgacgcgccg ctggtgttca
     6781 agggcgtcgt ctacaacgag atgaagggcg cgatgtcgag cccggtgcag gcgctggcgc
     6841 agcgcctgca gagcgcgctg ttcccgacca cgacctatca ccacaacagc ggcggcgagc
     6901 ccggggtcat tcccgatctc acgcatgaac gactcgtcgc cttccacggc acgcattacc
     6961 acccgtcgaa cgcggtgttc atgagctacg gtgacctgcc ggccgccgag ctgcagcagc
     7021 acttcgagga acgcgcgctg tcggcgttca cggcccggcc gctcgacctg gcgattccag
     7081 acgagcgccg ctacgacgcg ccggtcgcgg tcgaagcccc gtatgcggcc gatgccgagg
     7141 cgcttgccga cggcacgcac gtcgtgctcg gctggctgct cgggcgcacc accgatccgc
     7201 tcgaaaccgc gcgcgcgcgg gtgctttccg atgtgctgct cgacaactcc agctcgccgc
     7261 tgcgccacgc gctggagacc agcgaactcg gcgccgcccc gtcgccgctg tgcggcttcg
     7321 acgattcgac gcgcgagacc accttcacct gcggcctcga aggctgcgcg ccggacacgg
     7381 cggcggcggt cgagcagctc gtgctggaga ccatcacccg cgtcgcagag gacggcgtcg
     7441 cgcccgaggt cgtcgaatcg gtgctgcacc agctcgaact gcagagccgc gagatcagcg
     7501 gcgacggcta tccgtacggc ctgaagctga tgctcgatgc gctgacgccg ctgatgcacg
     7561 gcggcgatcc gctcgacggt ctcgacatcg atccggtgct cgccgagctg cgccgcgagg
     7621 ccgccgagcc cggcttcatc ccgcaactcg cgcgccggct gctgctcgac aatccgcacc
     7681 gcgtgcgcct gagcctgaag cccgatccgg aactcgccgc ccgtcaggcc gcggccgaga
     7741 tcgagcggct tgctgcgctg cgtgcgcagc tcgatgccga agcgcagcag caggtgcgcg
     7801 cgcaggctgc agccctcgcc gcgcgccagg cccgccatga cgatccggcg ctgctgccga
     7861 aggtcacgct cgccgacgtg ccggcggaac ttgcgattgc cacgggcgag acgcggcagc
     7921 tcaacggcgt cgagaccgcc tggtacgcgc agccgaccaa cggcctcgtc tatcagcagt
     7981 gggtcatcga cctgccggcg ctcgaccccg gactcgccgg cctcgtgccg ctgtacgcgc
     8041 tgacgctcag cgaggtcggc agcgccggtc gcgactatca ggaaacccag gccctgcagg
     8101 cggcggtgac cggcggcctc agcgcccgct gcctggtgcg cgccgcggtc gatgatccgc
     8161 agcagctgcg tgcggtgctg gtgctcgccg gcaaggcgct ggcgcgcaac gccggcgcgc
     8221 tgacgcagct gctgcgcgac acgctgctgg agccgcgctt cgacgaactc gaccgcctcg
     8281 ccgaactcgt cgcgcagtac cgcgcccagc gcgacgagga agtcgtcgac cacggccatg
     8341 cgctggcgat gggcgcggcc agcgaggcgc tgtcgccggc cggtgcgctg atctcgcagt
     8401 ggggcggcct gacggcgatc gaacggctgc gtgagctcga cgatgcgctg tccgcggatg
     8461 acgacgaagc ggatgcggcc ctcgcgacct acgcgcagcg gctcgcggcc ctgcacgccc
     8521 gggtgctgtg cgcgccgcgc cggctgctgg tggtcgccga agccggacgg catggcgagc
     8581 tggcggaact catcgccacg cacggcgccg gtccggccgc ggccggcacg gccgtgacac
     8641 tcgccccggc gctgcccgac agcccgccgg tgcaggcctg gacgctggcc acgcaggtca
     8701 gcttctgcgc ccgcgcctat ccggcggtcg cagcgggaca tccggatgca ccggcgctgg
     8761 tcgtgctcgg ggacttcctg cgcaatggct tcctgcaccg cgcgatccgc gagcgcggcg
     8821 gtgcctacgg cgccggcgcc ggcctgcacg ccgacagcgg cagcttccgc ttcttctcgt
     8881 accgcgatcc gcgtctggcc gagacgctgg ccgacttcga tgcctcgatc gactggctgc
     8941 tgagtacacg gcacgaagcc cagccgctgg aggaagccat cctcggcgtc gtcgccgcga
     9001 tcgaccgtcc cggctccccg gccggcgagg cgatcaatgc ctggttcagc gagctgttcg
     9061 ggcgcacgcc ggagcagcgc cggcgttttc gcgcccgcgt gctggcggtg acgatcgagg
     9121 atctgcagcg cgtcgcccgg acctggctgc gcccggagct ggcgcgcacg gcggtgatca
     9181 ccagcgccgc ggcgctggcc gcgagcgggc tgaacgcgga agtccggacg ctctgagaga
     9241 ccgctgccag accactcgtt tcagcccctc ctcctccggg ggaggggttg gggcgagggc
     9301 ttccttcggg cctcaatacg ccccggcaac catgattccg tgccggaaaa gccctcatcc
     9361 ccggcccttc ccccgtgggg gaaggtgaag cggccggcat tccgctactt gcgccgcgcc
     9421 cgggcaaacg catccgcgaa cgccccgccg ccggccggct tctgcgctct ggctggcggc
     9481 tgcgcctgtg gcggacggcc gctgcgggcc gtaccagccg gcttgcccgc gcccggctgg
     9541 tggtcgcgcg cctgatcggc cagccgcatc gtcagcgcga tgcgccggcg cttcacgtcg
     9601 acctccagca ccttcacgcg cacgacatcg ccggcgcgca ccacctggtg cgggtccttg
     9661 acgaacttct cggacagaca cgagacgtgc acgaggccgt cctgatgcac gccgatgtcg
     9721 acgaaggcgc cgaagttcgt gacgttggtg acgacgcctt cgagcaccat gtccgcttcg
     9781 aggtccttca ggtcctcgac gccttcgcgg aaggccgcag tgcggaactc cgggcgcggg
     9841 tcgcggccgg gcttttccag ctcggcgatg atgtcgcgca cggtcggcac gccgaagcgg
     9901 ccgtcggtgt actgctccgg acgcaggccg cgcaggaagc cggtgtcgcc gagcacgccg
     9961 ccgatgtcgc gcccggcgcg cttcaggatg cgctcgacga ccggataggc ctccgggtgc
    10021 acggccgaac ggtcgagcgg gttgtcgccg tgggcgatgc gcaggaatcc ggccgcctgc
    10081 tcgaaggtct tgtcgccgag gcgcggcacc ttcagcagct gcgcgcggtt gcggaaggcg
    10141 ccgttggcgt cgcgccacgc gacgatgttg cgcgccagcg tcgcgttcag gcccgacacg
    10201 cgcgtcagca gcggcaccga ggcggtgttg acatcgacgc cgacggcgtt gacgcagtcc
    10261 tc
//
LOCUS       DAOQIE010000124        10242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3290_length_10242_cov_7.198292, whole genome shotgun sequence.
ACCESSION   DAOQIE010000124 DAOQIE010000000
VERSION     DAOQIE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_10242_cov_7.198292"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..2671
                     /locus_tag="PK144_09240"
     CDS             470..2671
                     /locus_tag="PK144_09240"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNF65950.1"
                     /translation="MRMTTRFIASLLAVLAVVAVALSPLIGGLISRWFQRDIEIRSGL
                     VFHTLEAPLAERLGRHDADGIRTLFDDVDRDERVAGVGWCDARNVLVHRSRRMPDGFS
                     CRFAPATTGEPVFRSETSFGRQLLVGAFPLAGGRLVIVHDIGYITDRSASAQGYLVVS
                     LIVLAALISMVTVLVARLTLRDWLRTVRDSFAQPVASAAKPSSADLAPLVDEIRQRLR
                     ALDGVRRSVDDFRLNWAPETLAAILDRELPDSEVIVVSNREPYIHNLVDGRIDLQIPA
                     SGLVSALEPVIRACSGTWIAHGSGSADNLSVDAHDHIAVPPDAPAYTLRRVWLSEAEQ
                     DGYYYGLANEGLWPLCHIAFVRPVFRDGDWQQYVAVNRKFADAVIAEARTPRPIVLVQ
                     DYHFALLPRMVRERLPEATIVTFWHIPWPNAESFSICPWRGEILRGLLGSSILGFHTQ
                     FHCNNFLSAVDRFLESRIDQEEATVTHGGETTLVRHYPISIEWPPQAIRSLPPAAVCR
                     AQVQERLGIGADVRLGVGVERFDYTKGILDRFLAVEALLVNHPEWVGRFSFLQIAAPT
                     RNRLPVYQQLRAEAEALAERINARYGRPNWRPIVLLAEHHSPVEVFRLFRAADVCVVS
                     SLHDGMNLVAKEFVAARDDERGVLVLSTFAGASRELLEALIVNPYDTAGMARALVAGL
                     SMSGAEQGERMRLMREIVKENNVYRWAGRMLLDAARIRKRGHLLGWMETMN"
     gene            2795..3577
                     /gene="otsB"
                     /locus_tag="PK144_09245"
     CDS             2795..3577
                     /gene="otsB"
                     /locus_tag="PK144_09245"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNF65951.1"
                     /translation="MTDPTYLPPPPVPDAPWALFLDVDGTLIELAERPDAVAVPPELP
                     LLLNALDEALGGALALVSGRALAEVETLFAPHRYAIAGGHGAEWRPAADAAIECRPKP
                     ALAAARKRLEAFVCTHPGTLLETKPHALALHWRNAPAAESEALACMASVQAGLDSGFR
                     LLRGKSVCELLPEGFDKGAVVAGFCTSPPFAGRVPVFIGDDVTDEAGFAAVLARGGIA
                     VHVGTAGRPTCATRRLTDPAAVRAWLARDVLPACQRYGVPNP"
     gene            3574..5361
                     /locus_tag="PK144_09250"
     CDS             3574..5361
                     /locus_tag="PK144_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNF65952.1"
                     /translation="MSDLNLGILGNCIIAALVDTEARIVWGCLPRLDGDPVFCALLDN
                     GPDPERGGYWSLTLPNGRTREQAYLPNTAVLRTVIGDAEGNAIEILDFAPRHKQYGRM
                     YRPPMLVRCVRPLSGRPRIVTHLLPRFQNGELRPNITRGSNHARYVGPTQTLRLTTDA
                     PLSYVLEHVPFLLDRPMHFVLGPDEILNADIAETAGGLLARTRDYWRDWARSLTIPFE
                     WQDEVIRAAVTLKLCAYEETGAVVAALTTSIPEAAHSQRNWDYRFCWLRDAYFVIQAM
                     NRLGVTTTMEAYIGYIMDIVGDVETQGLGPLFAITRSADLSERVAPALAGYRGMGPVR
                     IGNEAWLQVQNDVYGSVVLAATHVFFDRRLELADRHGLFEQLERIGSRAVQVWDKPDA
                     GLWELRTREHVHTFSAVMCWAACDRLAKIAGVIGRRERSAYWRERADAMHARICAEAW
                     HEPGGYFASCFGGGDLDASLLLLNELDFLTADDPRFAATVDAIGTSLRSGDFLYRYQI
                     PDDFGRPETAFTVCAFWYVEALTALGRRDEARDLFGKLLARRNPLGLLSEDLDWHTGE
                     QWGNFPQTYSMVGLINCAMRLSIGWEEAF"
     gene            5465..5836
                     /locus_tag="PK144_09255"
     CDS             5465..5836
                     /locus_tag="PK144_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65953.1"
                     /translation="MHAADHPAAAWAARTVFDPVQIDCTTAVMLKILDGKCRMFEAEK
                     RAVAAVYAIVHKQPGRLLDAGVHALIRQAQAAPADAALRERVYEQRVLAETAISRPVM
                     KAYKAWLRAEGVIGGDGGAGD"
     gene            6058..6777
                     /locus_tag="PK144_09260"
     CDS             6058..6777
                     /locus_tag="PK144_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNF65954.1"
                     /translation="MLRDATPWLSIIVPVLDDAPALDVLLERLRPLRTQGVELIVVDG
                     GSRDDSVALACARSTCVLTAPAGRARQMNAGAALARGQALLFLHADSVPPDSVDALIG
                     AALGHGAHWGRFDVCLEGRDRRLGLVARLMNLRSRLSGIATGDQAIFVRADSFVQVGG
                     YPEQALMEDIELSRRLKRLGRPQCLAGPVRSSGRRWDEHGFWRTVTLMWWLRLAYRLG
                     LAPDRLARWYYGRGPRGTAGA"
     gene            6774..7409
                     /locus_tag="PK144_09265"
     CDS             6774..7409
                     /locus_tag="PK144_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNF65955.1"
                     /translation="MTAAGLALRLAVFAKAPLPGYAKTRLIPLLGAEGAARLAGTLIA
                     RTLALAEAVRPQALTLWCAPSAGHPYFRALAAGPSPPRLCTQTGADLGARMHAAFTDE
                     LARGPTLLIGTDCPALTAGHLHAAAEALLGTVAGPAADAVFLPAEDGGYVLVGLHTPQ
                     PRLFEDLPWSTPALMDATRARLRQAGLRGAEPATLWDLDRPADYRRWLKEG"
     gene            7533..8546
                     /locus_tag="PK144_09270"
     CDS             7533..8546
                     /locus_tag="PK144_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNF65956.1"
                     /translation="MPATPSSTAAVSALGPEDGLLYGYWLRPGLLAQRIGLAELDEAL
                     ATETGTVWLHFNLSNVRARRWLAQSERVPEEMKTALEERDDRPRIDRLDDGLFAVISD
                     FDYQEPGEFGLLRTLWIHAGTRLMLTARAHAVRSTDSLRVWVCGGLHMRSGIELFIEL
                     LSVRSDLLERLVDEMTEQVNEIEDAILTGGGAEREHLARIRRRCVRLRRTFGPERTAL
                     QKLDRQRAGWIDEDAIEQWHDATETLAFLLDDQSELYERAKLLQEEIASRVAEDTNRN
                     LYVLSVLSAVLLPMTLVTGIFGMNTAGLPFASGDHAFGWVLLIILAAGLGTAALLFFK
                     RLL"
     gene            complement(8530..>10242)
                     /locus_tag="PK144_09275"
     CDS             complement(8530..>10242)
                     /locus_tag="PK144_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNF65957.1"
                     /translation="AYGLSLWLWARGEPHLYFEAAAVIVALVLLGKVLEARARAGTGA
                     ALRELAALRPETACVLREGVETEVPVARLQVGDVCLVRPGARIPADGRVLEGRSHADE
                     SLLTGESVPVAKAPGDAVTGGALNGEGLLRIEVGAVGAESRLARIVRLVERAQAAKAP
                     VQRLADRVAAVFVPAVVGLAVLTVAGWLLAGAGLETALVNAVAVLVIACPCALGLATP
                     AAILVGTGVAARRGILIRDAAALERAQAVRLVAFDKTGTLTEGRPRLLAHDGPQAAAA
                     LRIAAALSSGSEHPLARALLDAAGSGLPAASGLQALPGRGVCGTIDGRGYRLGSRRLL
                     DEAGAVPGAALLETAAAAEAQGHTVSWLFETGETGAAGLPRVLGWFAFGDALRPGARE
                     AIAALHADGIDTVLISGDRRPAAEAIARELGIGQVIAEVLPEDKAAAIERLKGQGRIV
                     AMVGDGINDAPALAAADVGIALATGTAVAIEAAGITLMRADPRLVAEAIEVSRATLAK
                     IRQNLFWAFGYNVLGIPLAAFGGLSPMFAGAAMAFSSASVVGNALRLRRWQPAQAHTA
                     TADA"
BASE COUNT         1417 a         3719 c         3573 g         1533 t
ORIGIN      
        1 gcgttcatga cgccacctcc gggggcaagt cagggatcag acctgacctt gcctgtcggc
       61 cgggggggtg ggacatgatc tgcgtcagcg ggcgatctgc cggcatgtgg ctgacgtttc
      121 gtaatgcgga gtaaaaaaga cgtcacatcc ggccggaagc tctggatttc atgctgatgt
      181 atcgaattgc gaaatatgtt ccgatatgcg gaacaaaatg tcgggtcgat tgcatgacat
      241 gcatggatca cgatcgctgt ccggcgtgcg ggcggcatgg tgcagtgcct cgtcaggatg
      301 tgtcttgcgg cacgtttgct gctttaatcc cggggcgccc ctgacccggt ctgtccgccg
      361 gcagccttgg cacctgcttc agacttccgg ccccggcggt cgtgtcaggc cgatccgccc
      421 cgtgcgcgga cgtaccgcga ccgtgcacgc ctccccccgg aagctgccca tgcgcatgac
      481 cacccggttc atcgcatcgc tgctggcggt gctggccgtc gtcgccgtgg ccctgtcgcc
      541 actgatcggc ggcctgatct cgcgctggtt ccagcgcgac atcgaaatcc gttccggcct
      601 ggtgttccac acgctcgagg ccccgctggc cgagcgcctc gggcgtcacg acgccgatgg
      661 catccgtacg ctgttcgacg atgtcgatcg tgacgaacgt gtggccggcg tcggctggtg
      721 cgatgcccgc aacgtgctcg tgcatcgcag ccggcgcatg ccggacggct tctcgtgccg
      781 tttcgcgccg gcaaccaccg gcgagccggt cttccgcagc gagacctcgt tcggccggca
      841 gttgctggtc ggtgcgtttc cgctcgccgg cggccggctc gtgatcgtgc acgacatcgg
      901 ctacatcacc gaccgcagcg ccagtgccca gggttatctg gtcgtgtcgc tgatcgtgct
      961 cgcggcgctg atctcgatgg tgacggtgct ggtcgcacgg ctgacgctgc gcgactggct
     1021 gcgcaccgtg cgcgacagct tcgcgcagcc ggtggcgagc gcagcgaagc cgtcctccgc
     1081 cgatctggcg ccgctggtcg acgagatccg ccagcggttg cgcgcgctcg acggcgtgcg
     1141 ccgcagcgtc gatgacttcc ggctgaactg ggcaccggag acgctggcag cgatcctcga
     1201 tcgtgagctg ccggattccg aggtcatcgt cgtttccaac cgcgagccct acatccacaa
     1261 cctcgtcgac ggccggattg atctgcagat tccggcgagc ggcctggtct cggcgctgga
     1321 gccggtgatc cgtgcctgtt ccggcacctg gatcgcgcac ggcagcggaa gcgccgataa
     1381 cctgagcgtc gatgcacacg accacattgc cgtgccgccc gatgcaccgg cctacacgct
     1441 gcgccgcgtc tggctcagcg aggccgagca ggacggctac tactacgggc tcgccaacga
     1501 gggcctctgg ccgctctgcc acatcgcctt cgtgcgcccg gtcttccgcg atggcgactg
     1561 gcagcagtac gtggcggtca accgcaagtt tgccgacgcc gtcatcgccg aggcccgcac
     1621 gccgcggccg atcgtgctgg tgcaggacta ccacttcgcg ctgctgccgc gcatggtgcg
     1681 cgaacgtctg ccggaggcga cgatcgtgac cttctggcac atcccgtggc cgaacgccga
     1741 atcgttcagc atctgcccgt ggcgcgggga aatcctgcgc ggactgctcg gcagttccat
     1801 cctcggcttc cacacgcagt tccactgcaa caacttcctg tcggcagtcg accgcttcct
     1861 ggaaagccgc atcgatcagg aagaggcaac ggtcacgcac ggcggtgaga cgacgctggt
     1921 gcgccattat ccgatctcga tcgaatggcc gccgcaggcg atccgcagcc tgccgccggc
     1981 cgcggtctgc cgcgcacagg tgcaggaacg cctcgggatc ggcgccgacg tgcgcctcgg
     2041 cgtcggtgtc gagcgcttcg attacaccaa gggcatcctc gaccgctttc tggcggtcga
     2101 ggcgctgctg gtcaaccatc cggaatgggt cggccgcttc tcgttcctgc agatcgccgc
     2161 gccgacacgc aaccgcctgc cggtctacca gcagttgcgt gccgaggccg aggccctggc
     2221 cgagcgcatc aatgcccgtt acggccggcc gaactggcga ccgatcgtgc tgctcgccga
     2281 gcatcacagt ccggtcgaag tgttccggct gttccgtgcc gccgatgtct gtgtggtcag
     2341 cagcctgcac gacggcatga acctggtcgc caaggagttc gtcgccgcgc gtgacgacga
     2401 gcgcggcgtg ctggtcctca gcacctttgc cggggcctcg cgcgaactgc tggaggcgct
     2461 gatcgtcaat ccctacgaca ccgccggcat ggcccgggcg ctggtcgcgg ggctgtcgat
     2521 gtccggggcc gaacagggcg aacgcatgcg gctgatgcgc gagatcgtca aggagaacaa
     2581 cgtctatcgc tgggccgggc gcatgctgct cgacgccgca cgcatccgca aacgcggtca
     2641 tctgctcggc tggatggaga ccatgaactg aagctgcgtc cgcgcgccga gggaacgatc
     2701 ggcgcgtgct gctttctgcg ccagggatgc gcgcgcagac ctcaggccag cggcactgcc
     2761 cttgctcacc ccgtgccccc gagcagagaa gctgatgacc gatccgacgt acctgccacc
     2821 gccgcccgtt ccggatgctc cctgggcgct gtttctcgat gtcgatggca cgctgatcga
     2881 actggccgag cggcccgatg ccgttgccgt gccgccggag ctgccgctgc tgctgaacgc
     2941 cctcgacgag gcgctcggcg gcgcactggc gctggtcagc ggccgggccc tggccgaggt
     3001 cgaaaccctg ttcgcgccac accgctatgc gattgccggc ggccacggcg ccgaatggcg
     3061 gccggccgcc gatgcggcga tcgagtgcag gccgaagccg gcgcttgcgg ccgcgcgcaa
     3121 acggctcgaa gcctttgtct gcacgcatcc gggcacgctt ctcgaaacca aaccgcacgc
     3181 gctggcgctg cactggcgca acgcaccggc ggccgagtcc gaggcgctcg cctgcatggc
     3241 ctcggtacag gccgggcttg acagcggctt ccggctgctg cgcggcaagt cggtctgcga
     3301 gctgctgcca gagggcttcg acaagggggc agtcgttgcc ggattctgca ccagcccgcc
     3361 tttcgccggc cgtgtgccgg tgttcatcgg cgacgacgtc accgacgagg ccggctttgc
     3421 cgccgtgctc gcgcgcggcg gcatcgccgt gcatgtcggt accgccggtc gcccgacctg
     3481 cgccacccgc cgcctgaccg accccgccgc cgtgcgagcc tggctcgccc gcgacgtgct
     3541 gcccgcctgc cagcgttacg gagtcccgaa cccatgagcg acctgaatct cggcattctc
     3601 ggcaactgca tcatcgcggc cctggtcgac accgaggccc gcatcgtctg gggctgcctg
     3661 ccgcggctcg acggggatcc ggtgttctgc gcgctgctcg acaacgggcc ggacccggag
     3721 cgcggcggct actggagcct gacgctgccg aacggccgca cgcgcgagca ggcctatctg
     3781 ccgaacaccg cggtgctgcg caccgtgatc ggcgacgccg aaggcaatgc catcgagatc
     3841 ctcgatttcg cgccgcgcca caagcagtac gggcgcatgt accggccgcc gatgctggtg
     3901 cgctgcgtgc ggccgctgtc cggacggccg cgcatcgtca cgcacctgct gccgcgcttc
     3961 cagaacggcg agctgcgccc gaacatcacc cgcggcagca accacgcgcg ctacgtcggg
     4021 ccgacgcaga cgctgcgcct gaccaccgac gcaccgctgt cctacgtgct cgaacacgtg
     4081 cccttcctgc tcgaccggcc gatgcacttc gtgctcgggc cggacgagat cctgaacgcc
     4141 gacatcgccg agaccgccgg cggcctgctg gcgcgcacgc gcgactactg gcgcgactgg
     4201 gcgcgctcgc tgacgatccc gttcgaatgg caggacgagg tgatccgcgc cgcggtgacg
     4261 ctgaagctct gcgcctacga ggaaaccggc gccgtggtcg cggcgctgac cacctcgatc
     4321 ccggaggccg cgcattcgca gcgcaactgg gattaccgct tctgctggct gcgcgatgcc
     4381 tacttcgtga tccaggcgat gaaccgcctc ggcgtcacga cgacgatgga ggcctacatc
     4441 ggctacatca tggacatcgt cggcgatgtc gagacccagg gtctcggccc gctgttcgcg
     4501 atcacgcgca gcgccgatct ctccgagcgc gtcgcgccgg cgctcgccgg ctaccgcggc
     4561 atggggccgg tgcgcatcgg caacgaggcc tggctgcagg tgcagaacga tgtctacggc
     4621 agcgtcgtgc tcgctgcgac ccacgtgttc tttgaccgcc ggctcgaact cgccgaccgt
     4681 cacggcctgt tcgaacagct cgaacgcatc ggctcgcgcg ccgtgcaggt ctgggacaag
     4741 cccgatgccg ggctctggga gctgcgcacg cgcgagcacg tgcacacgtt ctcggcggtg
     4801 atgtgctggg cagcctgcga ccggctcgcc aagatcgccg gcgtgatcgg ccggcgcgaa
     4861 cgctcggcct actggcgcga gcgcgccgat gcgatgcacg cgcgcatctg cgccgaggcc
     4921 tggcacgagc cgggcggcta cttcgcgtcc tgcttcggcg gcggggacct cgatgcctcg
     4981 ctgctgctgc tgaacgaact cgacttcctc acggctgacg atccgcgctt cgcggccacc
     5041 gtcgatgcga tcggcaccag cctgcgctcc ggcgacttcc tgtaccgcta ccagattccg
     5101 gacgacttcg gccggcccga gaccgcgttc acggtctgcg cgttctggta cgtcgaggcg
     5161 ctgaccgcgc tcgggcgccg cgacgaggcc cgcgacctgt tcggcaagct gctggcgcgg
     5221 cgcaaccccc tcggcctgct gtccgaggat ctcgactggc acaccggcga gcagtggggc
     5281 aacttcccgc agacctactc gatggtcggg ctgatcaact gcgcgatgcg gctgtcgatc
     5341 ggctgggaag aagccttctg agcccgccct gcggcagcgg ccgccgctga aggcgcgccg
     5401 cgcttgcagc cggcgcggcg ctggcgtctg ctgggctttc cgatcgccga cccggagccg
     5461 cgccatgcac gctgccgatc atcctgccgc cgcctgggcg gcccgcaccg tcttcgatcc
     5521 cgtgcagatc gactgcacga cggcggtgat gctgaagatc ctcgacggca agtgccgcat
     5581 gttcgaggcc gagaagcgcg cggtcgcggc ggtctacgcc atcgtccaca agcagccggg
     5641 gcggctgctc gatgccggcg tgcatgcgct gatccgtcag gcgcaggcgg caccggcgga
     5701 tgccgcgctg cgggagcgcg tctacgaaca gcgcgtgctc gccgagaccg cgatctcgcg
     5761 gccggtcatg aaggcctaca aggcctggct gcgcgccgaa ggcgtgatcg gcggcgacgg
     5821 gggcgctggc gactgaccca cgccgaccgc cgcgcgcacc cgatgcgacc tcgatgtgac
     5881 ctcgatgcga tcacaacgat ggtggcgcgg cgcagtgggc gggatcggcc gtgctatacg
     5941 tccccggcat gcccgtcgat cgagtcgccc gatgatgccc gtgcccccgc catccgtccg
     6001 caccggagcg cacccgtgag tgccgtcccg gattccgccg gacacccggc tgcgtcgatc
     6061 ctgcgcgatg ccacgccgtg gctgtcgatc atcgtgccgg tgctcgacga tgcgccggcc
     6121 ctcgatgtgc tgctggagcg gctcaggccg ctgcgcacgc agggggtcga gctgatcgtc
     6181 gtcgatggcg gcagccgcga tgattcggtg gcgctcgcct gcgcgcgcag cacctgcgtg
     6241 ctcacggcgc ctgccgggcg cgcgcgacag atgaacgccg gcgcggcgct ggcacgcggt
     6301 caggcgctgc tgttcctgca cgccgacagc gtgccgcccg acagcgtcga tgcgctgatc
     6361 ggcgccgcgc tcggccacgg tgcgcactgg gggcgcttcg acgtatgcct cgaaggccgc
     6421 gaccgccggc tcggactggt cgcgaggctg atgaacctgc gctcgcgcct gagcggcatc
     6481 gccaccggcg accaggcgat cttcgtgcgc gccgacagct tcgtgcaggt cggcggctat
     6541 ccggagcagg cgctgatgga ggacatcgag ctgagccggc gcctcaagcg cctcggccgg
     6601 ccgcagtgcc tggccggacc ggtgcgcagc tccggccggc gctgggacga gcacggcttc
     6661 tggcgcaccg tgacgctgat gtggtggctg cggctggcct accggctcgg tctggcgccg
     6721 gaccggctgg cgcgctggta ctacggacgg gggccgcggg gcacggccgg cgcatgacgg
     6781 cggccgggct ggcgctgcgc ctggccgtct tcgccaaggc accgctgccg ggctacgcca
     6841 agacgcggct gattccgctg ctcggcgccg agggcgcggc gcggctcgcc ggaaccctga
     6901 tcgcacgcac gctcgcgctc gccgaagccg tgcggccgca ggcgctgacg ctctggtgcg
     6961 caccgtcggc cgggcatccg tacttccgcg cgctggcggc cgggccaagt ccgccgaggc
     7021 tctgcacgca gaccggcgcc gatctcggcg cgcgcatgca cgcggccttc accgatgaac
     7081 tcgcccgcgg tccgacgctg ctgatcggca ccgactgccc ggcgctcacg gccgggcacc
     7141 tgcacgccgc ggccgaggcg ctgctcggga ccgtcgccgg tccggcggcc gatgccgtgt
     7201 tcctgccggc cgaggatggc ggttacgtgc tggtcgggct gcacacgccg cagccgcggc
     7261 tcttcgaaga cctgccgtgg agcacgccgg cgctgatgga cgcgacgcgc gcgcgcctgc
     7321 ggcaggccgg cctgcgcggg gccgagccgg cgacgctgtg ggacctcgac cggcccgcag
     7381 actaccggcg ctggctgaag gaaggctgaa gcgcccgggc gccgatgatg cacctgcaca
     7441 atgacggcgc tgcggatcgg ttacgatgcc gcaaaccccg gccgccatcc gccggccggt
     7501 cctgccgcct ccagcttcgg accctccctc ccgtgccagc cacgccgtct tcgactgccg
     7561 ccgtttccgc cctcggtccc gaggacggcc tgctgtacgg ctactggctg cgtccggggc
     7621 tgctcgcgca gcgcatcgga ctcgccgagc tcgacgaggc gctcgccacc gagaccggca
     7681 cggtctggct gcacttcaac ctgagcaacg tccgcgcccg gcgctggctc gcgcagtcgg
     7741 agcgcgtgcc cgaggagatg aagacggcgc tggaggagcg cgacgaccgt ccgcgcatcg
     7801 accggctcga cgacggcctg ttcgcggtga tcagcgactt cgattatcag gagcccgggg
     7861 aattcgggct gctgcgcacg ctgtggatcc acgccggcac gcggctgatg ctgaccgcgc
     7921 gcgcccacgc ggtgcgcagc accgacagcc tgcgcgtctg ggtctgcggc ggcctgcaca
     7981 tgcgcagcgg catcgagctg ttcatcgagc tgctgtcggt gcgctccgac ctgctcgaac
     8041 gcctggtcga cgagatgacc gagcaggtca acgagatcga ggacgcgatc ctgaccggcg
     8101 gcggcgccga gcgcgaacac ctcgcgcgca tccgccgccg ctgcgtgcgc ctgcgccgca
     8161 ccttcggccc ggagcgcacg gcgctgcaga agctcgaccg ccagcgcgcc ggctggatcg
     8221 acgaggacgc catcgagcag tggcacgacg cgaccgagac gctggccttc ctgctcgatg
     8281 accagagcga gctctacgag cgcgccaagc tgctgcagga agaaatcgcc tcgcgcgtcg
     8341 ccgaggacac caaccgcaac ctgtacgtgc tgtcggtgct gtcggccgtg ctgctgccga
     8401 tgacgctggt caccggcatc ttcggcatga acaccgccgg cctgccgttc gcgagcggcg
     8461 atcacgcctt cggctgggtg ctgctgatca tcctcgccgc cggcctcggc acggcggcgc
     8521 tgctgttctt caagcgtctg ctgtagccgt gtgcgcctgc gccggctgcc agcggcgcag
     8581 gcgcagcgcg ttgccgacca cgctcgcgct cgagaacgcc atcgccgcgc cggcgaacat
     8641 cggcgacagc ccgccgaagg ccgcgagcgg gatgccgagc acgttgtagc cgaaggccca
     8701 gaacaggttc tggcggatct tcgcgagcgt cgcgcgcgag acctcgatcg cctcggcgac
     8761 caggcgcgga tcggcgcgca tcagcgtgat gccggcggcc tcgatcgcga ccgcggtgcc
     8821 ggtcgcaagc gcgatgccga catcggccgc ggccagcgcc ggcgcgtcgt tgatgccgtc
     8881 cccgaccatc gccacgatcc ggccctgacc cttcaggcgc tcgatcgcgg cagccttgtc
     8941 ctcgggcagc acctcggcga tgacctgccc gatgccgagt tcgcgggcga tggcctcggc
     9001 cgccgggcgg cggtcgccgg agatcagcac ggtgtcgatg ccatcggcgt gcagcgcagc
     9061 gatggcttcg cgggcacccg gacgcagcgc gtcgccgaac gcgaaccagc cgagcacgcg
     9121 cggcaggccg gccgcgccgg tttcgccggt ttcgaacagc caggacacgg tatggccctg
     9181 cgcttctgcg gccgcggcgg tttcgagcag cgcggccccg gggacggcgc cggcctcatc
     9241 gagcaggcga cggctgccga gtcgatagcc gcgtccatcg atcgtgccgc atacgccgcg
     9301 accgggcagg gcctgcagcc cgctggcggc cggcagccca ctgccggcgg catcgagcag
     9361 cgcgcgcgcc agcggatgct cggagccgct cgacagcgcc gcggcgatcc gcagcgccgc
     9421 ggccgcttgc gggccgtcgt gcgcgagcag ccgcggccgg ccttcggtca gcgtgccggt
     9481 cttgtcgaag gcgaccagcc tcacggcctg cgcgcgttcg agggccgcgg catcgcggat
     9541 caggatgccg cgccgggccg cgacgccggt gccgaccagg atcgccgccg gcgtcgccag
     9601 cccgagcgcg cacgggcagg cgatgaccag caccgcgacg gcattgacca gcgccgtctc
     9661 aagcccggcc ccggccagca gccagccggc gacggtcagc accgcgaggc cgacgacggc
     9721 cggcacgaac acggcggcca cgcgatcggc gagccgctgc accggggcct tcgccgcctg
     9781 cgcgcgttcg acgaggcgca cgatgcgcgc gagcctggat tcggcgccga cggcgccgac
     9841 ctcgatgcgc agcaggccct cgccgttcag cgcgccgccg gtgacggcat cgccgggggc
     9901 tttcgcgacc gggaccgatt cgccggtcag caggctctcg tcggcgtggc tgcggccttc
     9961 gagcacgcgg ccgtcggccg gaatgcgcgc gccggggcgc acgaggcaga catcgccgac
    10021 ctgcagccgt gcgaccggca cttcggtctc gacgccttcg cgcagcacgc aggcggtttc
    10081 gggccgcagc gccgccagtt cgcgcagcgc cgcgccggtg ccggcgcgcg cgcgggcttc
    10141 gagcaccttg ccgagcagca ccagcgcgac gatcacggcg gcggcctcga agtacagatg
    10201 cggctcgccg cgcgcccaga gccacagcga caggccgtag gc
//
LOCUS       DAOQIE010000125        10214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3305_length_10214_cov_8.162319, whole genome shotgun sequence.
ACCESSION   DAOQIE010000125 DAOQIE010000000
VERSION     DAOQIE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_10214_cov_8.162319"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="PK144_09280"
     CDS             complement(<1..1483)
                     /locus_tag="PK144_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNF65958.1"
                     /translation="MTPAGVKIVRHISHGLREIGFLLLATVAAFLFIALASYVPADPA
                     WTHTGTSAPMNLGGVAGAWFADIALYFFGYPAYLFPLGLVFAGWRLFKRNGLAEIDAE
                     IIVFRTVGFLVTVGTGCGLAALHLQPGALRGSAGGIVGDLCGHGLINAFGPGGGTLFL
                     AALFLAGVTLASGLSWLSLVDLIGKSIWDVAALFARAGSWVAERIAPRRDEAGDDEPL
                     QQGRAPELSLAGGRQEPVLVAGLPPQAPPVPVSKPVTEPEKPATGSDGPAEPATAGKR
                     GRIEPSFDATTAPWDLAEDDEPKPPLPVTRTSVPTVRPLDDDEDDDGLGVPAPEALPA
                     TVAVRTGEPESDMGMDMDDDETPSQTQSAPQAGGARPLPPAAVATDEDEDEHENARSL
                     PAAVPDSAELVIGARHNAPIEVPVAQPIAAAVPTPAPTPLPAPVPALVAVPAVPAIAP
                     RPQPRAEGVSEAAAALFGLRPKREALDPLPPLELLDLPREQAAG"
     gene            1758..2906
                     /locus_tag="PK144_09285"
     CDS             1758..2906
                     /locus_tag="PK144_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF65959.1"
                     /translation="MHLRCHESFRELPAAEWNTLVRDEDPFLQHAFLVALENHGVTGP
                     GTHWQPRPLSVRDDDGRLLGGCPLFLKTDSWGEFVFDWGWADAYHRNGLRYYPKLVAA
                     IPHTPATGQRLLVAAGADAGAVMALLAEGARELAAGLGCSTLHVLFPQAGELESLAAA
                     GLTHRLGCQFHWQDRGYRDFDDFLDAFSAKKRKNLRQERRRVREAGLELERLPGNAVS
                     EAQWRVFHRFYRNTFEQRGCPAPMSLPFFLEIGRTLGERVLLVLARRGHDIVAAAISY
                     AGAHTLYGRHWGCAESFDALHFEACYYQGIEHCIEHGLTRFEPGAQGEHKVARGFEPV
                     LTHSAHWVAHPGFRQAIDAFLHRERPAIVEYARELTARLPYRSGEAAA"
     gene            2903..3619
                     /gene="aat"
                     /locus_tag="PK144_09290"
     CDS             2903..3619
                     /gene="aat"
                     /locus_tag="PK144_09290"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNF65960.1"
                     /translation="MIAWLDARNPNQPFPPAEAALVEPNGLLAAGGSLAPARLLNGYR
                     QGIFPWYGKGQPILWWSPDPRTIFLPDRIRVSHSLRKVLRSGRYTVTVDRAFACVIAE
                     CAAPRPEAPESWITREMREAYCRLHVLGYAHSIETWHGERLVGGLYGVALGGVFFGES
                     MFSRMNDASKVALVHLGRLLVQRGFGFIDCQLETGHLLSLGAQSLPRAEFLKLLHEHV
                     HRLLPATPWTLPAEAAPGAG"
     gene            3622..4356
                     /locus_tag="PK144_09295"
     CDS             3622..4356
                     /locus_tag="PK144_09295"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNF65961.1"
                     /translation="MAAPRSELGSLRLFLTDAHPCSYLAGHTARNLVADPLAVCDTLY
                     SALVLHGFRRSGEHVYRPHCQGCHACQSLRIPVRQFQPNRSQRRTQARNLDLQVSLVE
                     PVLREEHFQLFARYLALRHPDGGMDDATPDSYLGFIRSSWSRTFLAEFRDADGRLLAV
                     AVVDVLGDGLSAVYTYYDPAAEARAPGTAAILWQIEEARRRGLAYVYLGYWIAGCRKM
                     NYKTRFRPCEVFINGDWRSPPETPVR"
     gene            4521..4739
                     /gene="infA"
                     /locus_tag="PK144_09300"
     CDS             4521..4739
                     /gene="infA"
                     /locus_tag="PK144_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNF65962.1"
                     /translation="MSKEDHIEMEGTVIETLPNTMFRVQLENGHVVTAHISGRMRKHY
                     IRILTGDKVRVELTPYDLSKGRITYRGR"
     gene            complement(4806..7067)
                     /gene="clpA"
                     /locus_tag="PK144_09305"
     CDS             complement(4806..7067)
                     /gene="clpA"
                     /locus_tag="PK144_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNF65963.1"
                     /translation="MLSKELEFTINLAFREARDRRHEFMTVEHLLLALLDNPSAAEVL
                     RACGANMDKLKSDLQGFIRESTPVLAADDPRETQPTLGFQRVLQRAVLHVQSSGNKEV
                     TGANVLVAIFGEQDSQAVYFLKRQDISRLDVINYISHGISKVSDDGDHGNAAQNSEEE
                     PGEQPSGRPLDNYASNLNEMARQGRIDPLIGRAEEVERTIQILCRRRKNNPLFVGEAG
                     VGKTAIAEGLAKLIVEGKVPDVLKKATIYSLDLGSLVAGTKYRGDFEKRLKAVLTQLR
                     KERNAVLFIDEIHTVIGAGAASGGVMDASNLIKPALASGDLKCIGSTTYQEYRGIFEK
                     DRALARRFQKIDVPEPSVEETYQILKGLKPRFEEHHDVKYAPKALRAAAELSARYIND
                     RHLPDKAIDVIDEAGASQRLLTPNRRKKVIGVPEIEAIVAKIARIPPKSVSASDKDTL
                     KNLDRNLKLVVYGQDQAIGALSNAIKMARSGLGNEMKPIGAFLFAGPTGVGKTEVTRQ
                     LAKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAILKHPHAVLLLDE
                     IEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRNVILVMTTNAGADQIERSGIGFTTED
                     HSTDSLEVIKRMFSPEFRNRLDAIIQFKALESDTIGHVVDKFIIELEAQLVAKHVTLE
                     VSGAARAWLAVRGYDRKMGARPMARVIQEHIKKPLAEELLFGRLASGGHVLIDADGDG
                     LVIDFAEATAEVS"
     gene            complement(7123..7446)
                     /gene="clpS"
                     /locus_tag="PK144_09310"
     CDS             complement(7123..7446)
                     /gene="clpS"
                     /locus_tag="PK144_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNF65964.1"
                     /translation="MAQEHNPRHGDGGLAVEKAKPELKPPRMYKVVLLNDDYTPMEFV
                     VHVLEAFFGMNREQATQVMLHVHTRGKGVCGVYTRDIAETKVAQVNAFSRTHQHPLLC
                     TMEEA"
     gene            complement(7630..8904)
                     /gene="aceA"
                     /locus_tag="PK144_09315"
     CDS             complement(7630..8904)
                     /gene="aceA"
                     /locus_tag="PK144_09315"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNF65965.1"
                     /translation="MPHLSLEQLKNDWAENPRWKGITRAYSAEDVVRLRGTAGVEYTL
                     ATKGAEKLWKYLNTEPFINALGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGAMY
                     PDQSLYPANSVPAVVERINNTLMRADELHHAEGNDSIDFFQPIVADAEAGFGGVLNAF
                     ELMKAMIKAGASGVHFEDQLASVKKCGHMGGKVLVPTREAVEKLTAARLAADLMGVPT
                     LLVARTDAEAADLLTSDVDDNDKPFCTGERTVEGFYRVKPGLEQAISRGLAYAPVADL
                     VWCETGKPDLEYARKFAEAIHAKFPGKMLAYNCSPSFNWKKNLDDATIAKFQRELGAM
                     GYKFQFITLAGFHSLNYSMFNLAYGYARQNMSAFVELQEAEFKAAEKGFTAVKHQREV
                     GTGYFDSVTQTIQGGQSSTVALKGSTEEEQFH"
     gene            9115..10056
                     /locus_tag="PK144_09320"
     CDS             9115..10056
                     /locus_tag="PK144_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF65966.1"
                     /translation="MNSPLPRHYYKHNRLQQLRGFCYAVQTGSISRAAERMFLSQPSV
                     SLQIQALERELGVTLFERRGPRIRLTPEGQTLYEMALPLLEGLDGLPETFLARRGSVE
                     SGELNITAGEATILYLLPDVVTRFSAAHPGIRVRFHNVPGREGLRLLREDEVDFAVGS
                     FREVPDDMSYQPIHAYDQTLITPLEHPLAGRLEVSLADISPYEFILPPRGLNTLSMVE
                     LAFQAQNLSLNVRLEAGGWAVIKRYVEMGLGISIVSGICLTPDDKLSSLSLNRYFPRR
                     TYGVAMRRGKFLTPQAKRFIEFIDPNFFAPDTDPADA"
BASE COUNT         1599 a         3532 c         3333 g         1750 t
ORIGIN      
        1 agccagcggc ctgctcgcgc ggcaggtcca gcagttcgag cggcggcagc ggatcgagcg
       61 cctcgcgctt cggccgcagc ccgaacagcg ccgccgcggc ctcggacacg ccctcggcac
      121 gcggctgcgg tcgcggtgca atcgcaggaa ccgcaggaac cgccactagc gcggggactg
      181 gcgccggcag cggtgtcggg gccggagtcg ggacagccgc agcaatcggc tgcgccaccg
      241 gcacctcgat cggcgcgttg tgccgtgccc cgatcaccag ctcggcagag tccggcaccg
      301 ctgccggcaa cgacctggcg ttctcgtgct cgtcctcatc ttcgtccgta gccaccgcag
      361 ccggcggcaa cgggcgtgct ccgccggctt gcggtgcgga ctgcgtctgt gacggcgtct
      421 catcgtcgtc catgtccatg cccatgtccg attccggctc gccggtacgc acagccacgg
      481 ttgccggcaa tgcctccggc gccggtacgc ccagcccgtc gtcatcctcg tcatcgtcga
      541 gcggacgaac cgtcggtacg gacgtacggg tcaccggcag cggcggcttc ggttcgtcgt
      601 cctctgcgag gtcccacgga gccgtcgtcg catcgaagga cggttcgatc cggccgcgct
      661 tgccggcggt agccggctcg gccgggccgt cgctgccggt ggcaggtttt tccggttccg
      721 tgaccggctt cgacaccggc acaggcggcg cctgcggcgg cagccctgca accagcaccg
      781 gctcctgccg gccgccggcc agcgacagct cgggcgccct gccctgctgc agtggctcat
      841 cgtcgccggc ttcgtcccgg cgcggagcga tgcgctcggc aacccagctg ccggcccggg
      901 cgaacagcgc cgcgacatcc cagatcgact tgccgatcag atcgaccagc gacagccacg
      961 acagccccga ggccagcgtc acgccggcga ggaacagcgc ggccaggaac agcgtgccgc
     1021 caccgggtcc gaaggcgttg atcagcccgt gcccgcacag atcgccgacg atgccgccgg
     1081 cactgccgcg cagggctccg ggctgcaggt gcagggcggc cagtccgcag ccggtgccga
     1141 ccgtgaccag aaagccgacg gtacggaaca cgatgatctc ggcgtcgatc tcggcaaggc
     1201 cgttgcgctt gaacagccgc cagcccgcga acaccaggcc gagcgggaac aggtaggcgg
     1261 ggtagccgaa gaagtacagc gcgatgtcgg caaaccacgc gccggccacg ccgccgaggt
     1321 tcatcggtgc actggtgccg gtatgcgtcc aggccggatc ggccggcacg tacgaggcca
     1381 gtgcgatgaa caggaaggcc gccacggtcg cgagcagcag gaagccgatc tcgcgcaggc
     1441 catggctgat gtgacggacg atcttgacgc ccgcgggcgt cacgtttttt ctgcgtgcct
     1501 gctgagccaa gctgccacct gtgtgaagcg atctggggat gggtccgtgc cgcgcaaggc
     1561 gcgtcgaagc ggacggcagg gcggcggagc agccggaggg gtgatgaggg gatgcattgt
     1621 cccatcccgc cccggtgctg gcaaagcgcg caccacgtgc gcggccgtcg acttgcccct
     1681 aagcttggaa tcgggtgtca aacttcgccg cgcccggcgc gtcgaagcgg caccggtcac
     1741 acgagccccc caagagcatg cacctgcgct gccacgaatc cttccgcgag ctgcccgctg
     1801 ccgagtggaa caccctggtc cgcgacgaag acccctttct tcagcatgcg tttctcgtcg
     1861 cgctcgaaaa ccacggcgtc accggccccg gcacgcactg gcaaccacga ccgctgagcg
     1921 tgcgcgacga tgacggccgc ctgctcgggg ggtgtccgct gttcctgaag accgactcct
     1981 ggggcgagtt cgtgttcgac tggggctggg ccgatgccta ccaccgcaac ggcctgcgct
     2041 attacccgaa gctggtggcg gcgatcccgc acacgccggc gaccggccag cggctgctgg
     2101 tcgccgccgg tgccgatgcc ggcgcggtca tggcgctgct tgccgaaggt gcgcgcgaac
     2161 tcgccgccgg gctcggctgc tcgacgctgc acgtgctgtt cccgcaggcc ggtgaactcg
     2221 aatccctggc ggcggccggg ctcacgcacc ggctcggctg ccagttccac tggcaggatc
     2281 gcggctaccg cgatttcgac gatttcctcg acgccttctc ggccaagaag cgcaagaacc
     2341 tgcgccagga acgccggcgc gtgcgcgagg ccggcctcga actcgaacgc ctgcccggga
     2401 atgccgtcag cgaggcgcag tggcgggtct tccaccgctt ctaccgcaac accttcgagc
     2461 agcgcggctg tccggccccg atgagcctgc cgttcttcct ggagatcggc cgcacgctcg
     2521 gcgaacgcgt gctgctggtg ctcgcccggc gcgggcatga catcgtcgcc gccgcgatca
     2581 gctacgccgg tgcgcacacg ctgtacggcc gccactgggg ctgcgccgag tccttcgacg
     2641 cgctgcactt cgaagcctgt tactaccagg gtatcgagca ctgcatcgag cacgggctga
     2701 cacgcttcga acccggcgcg cagggcgagc acaaggtggc gcgcggcttc gaaccggtgc
     2761 tgacgcactc ggcgcactgg gtcgcacatc cgggcttccg tcaggcgatc gacgccttcc
     2821 tgcatcgcga gcgcccggcc atcgtcgagt acgcacgcga actcaccgca cggctgccgt
     2881 atcgcagcgg cgaggccgcg gcatgatcgc ctggctcgac gcccgcaatc cgaaccagcc
     2941 gtttccgccc gccgaggcgg cgctggtcga accgaacggc ctgctcgccg ccggcggcag
     3001 cctggcgccg gcccggctgc tgaacggcta ccggcagggc atcttcccgt ggtacggcaa
     3061 gggccagccg atcctgtggt ggtcaccgga cccgcgcacg atcttcctgc ccgaccgcat
     3121 ccgggtctcg cacagcctgc gcaaggtgct gcgcagcggt cgctacacgg tcacggtcga
     3181 tcgcgccttc gcgtgcgtga tcgccgaatg cgccgcaccg cgaccggagg ccccggaatc
     3241 ctggatcacc cgcgagatgc gcgaggccta ctgccggctg cacgtgctcg gctacgcgca
     3301 ttcgatcgag acctggcacg gcgagcgtct ggtcggcgga ctgtatggcg tcgcgctcgg
     3361 cggcgtgttc ttcggcgaat caatgttcag ccgcatgaac gatgcgtcca aggtcgcgct
     3421 cgtgcatctc ggccggctgc tggtgcagcg cggcttcggc ttcatcgact gccagctcga
     3481 aaccggccat ctgctgagcc tcggcgcgca gagcctgcca cgcgccgagt tcctgaagct
     3541 gctgcacgag cacgtccacc gcctgctgcc ggccacgccg tggacgctgc cggccgaagc
     3601 agcgcccgga gccggctgac gatggccgct ccgcgctccg aactcggcag cctgcggctg
     3661 ttcctgaccg atgcgcatcc gtgcagctat ctggccggcc acacggcgcg caacctcgtc
     3721 gccgacccgc tcgccgtctg cgacacgctg tactcggcgc tggtgctgca cggtttccgg
     3781 cgcagcggcg agcacgtcta ccggccgcac tgccagggct gccacgcctg ccagtcgctg
     3841 cgcatcccgg tccggcagtt ccagccgaac cgctctcaac ggcgcacgca ggcccgcaac
     3901 ctcgacctgc aggtcagcct ggtcgaaccg gtgctgcgcg aggagcactt ccagctgttc
     3961 gcccgctacc tggcgctgcg ccacccggac ggcggcatgg acgatgccac ccccgacagc
     4021 tacctcggct tcatccgttc cagctggagc cggaccttcc tcgccgaatt ccgcgacgcc
     4081 gacggccggc tgctggcggt cgcggtcgtc gacgtgctcg gggacggact gtcggcggtc
     4141 tacacgtatt acgatcccgc ggccgaagcg cgggcacccg gcaccgcggc gatcctctgg
     4201 cagatcgagg aggcgcgccg ccgcggtctg gcctacgtct acctcggata ctggattgcc
     4261 ggctgccgca agatgaacta caagactcgc ttccggccct gcgaggtctt catcaacggc
     4321 gactggcgct ccccgcccga aacgcctgtc cgctgaaccg cccggccgcg accgtccacc
     4381 ctcgcgtacg aattcaccct gtcccgtgct gccggtcctt gatgctgaag ggccgtttgg
     4441 ctattttggc gcgccgcacc agaccgaccg caccgaccga tcgccctcac cgccgctttt
     4501 ccccctgcca gagagtcctg atgtcgaaag aagaccatat cgaaatggaa ggaacggtca
     4561 tcgagaccct tccgaacacg atgttccgcg tacagctcga aaacggccac gtcgtcaccg
     4621 cccacatttc cggccgcatg cgcaagcact acatccgcat cctgaccggc gacaaggtcc
     4681 gcgtcgaact gacgccgtac gacctgtcga aaggccgcat cacctatcgc ggccgctgaa
     4741 aaacaagaac cggcagggtc gcccccgccg gtccggtgca gcacgcggcg tcagcgccgg
     4801 tccggtcagc tcacttccgc cgtcgcctcg gcgaaatcga tcaccagccc gtcaccgtcg
     4861 gcatcgatca gcacgtgccc gcccgaggcg aggcgtccga acagcagttc ctcggccagc
     4921 ggcttcttga tgtgctcctg gatcacgcgc gccatcggcc gggcgcccat cttgcggtcg
     4981 tagccgcgca cggcgagcca ggcgcgggcg gcgccggaga cttccagcgt cacgtgcttg
     5041 gcaaccagct gcgcttcgag ctcgatgatg aacttgtcga cgacgtggcc gatcgtgtcg
     5101 ctctccagcg ccttgaactg gatgatcgca tcgaggcggt tgcggaactc cggcgagaac
     5161 atgcgcttga tgacttcgag gctgtcggtc gagtgatcct cggtcgtgaa gccgatgccg
     5221 gagcgctcga tctggtctgc accggcgttg gtggtcatga ccaggatgac gttgcggaaa
     5281 tccgccttgc ggccgttgtt gtcggtcagc gtgccgtgat ccatgacctg cagcagcagg
     5341 ttgaacacgt ccgggtgcgc cttctcgatc tcgtcgagca gcagcacggc atgcgggtgc
     5401 ttcaggatcg catcggtcag caggccgccc tggtcgaagc cgacgtagcc gggcggtgcg
     5461 ccgatcagcc gtgacaccgt gtgccgctcc atgtactcgg acatgtcgaa gcgcagcagc
     5521 tcgatgccga gcgccttggc gagctggcgc gtgacctcgg tcttgccgac gccggtcggg
     5581 ccggcgaaca ggaaggcacc gatcggcttc atctcgttgc caagcccgga gcgcgccatc
     5641 ttgatcgcgt tcgacagcgc cccgatcgcc tgatcctggc cgtagacgac cagcttcagg
     5701 ttgcggtcga ggttcttcag cgtgtccttg tcggaagccg agacgctctt cggcgggatg
     5761 cgcgcgatct tggcgacgat ggcctcgatc tcgggcacgc cgatgacctt cttgcgccgg
     5821 ttcggcgtca gcagccgctg gctggcgccg gcctcgtcga tgacatcgat cgccttgtcc
     5881 ggcaggtggc ggtcattgat gtagcgcgcc gacagctcgg ccgcggcacg cagcgccttc
     5941 ggtgcgtact tgacgtcgtg gtgttcctcg aagcgcggct tcaggccctt caggatctgg
     6001 taggtctcct cgaccgaagg ctccggcacg tcgatcttct ggaagcgccg tgccagcgcg
     6061 cgatccttct cgaagatgcc gcggtattcc tggtaggtcg tcgagccgat gcacttcagg
     6121 tcaccggagg cgagcgccgg cttgatcagg ttcgaggcgt ccatgactcc gcccgaggcc
     6181 gcaccggcgc cgatgacggt gtggatctcg tcgatgaaca gcacggcgtt gcgctccttg
     6241 cgcagctgcg tcagcaccgc cttcaggcgc ttctcgaagt cgccccgata cttggtgccg
     6301 gcgaccagcg agccgaggtc gagcgagtag atcgtcgcct tcttcagcac gtcgggcacc
     6361 ttgccctcga cgatcagctt cgcgagtcct tcggcgatcg cggtcttgcc gacgccggcc
     6421 tcgccgacga acagcgggtt gttcttgcgc cgccggcaga ggatctggat cgtgcgctcg
     6481 acttcctcgg cccggccgat cagcggatcg atgcgcccct gacgggccat ctcgttgagg
     6541 ttgctggcgt agttgtcgag cggccggccg ctcggctgct cgccgggttc ctcttcggaa
     6601 ttctgggcgg cattgccgtg atcgccgtcg tcggacacct tggaaatccc gtgcgagatg
     6661 tagttgatga cgtcgagccg gctgatgtcc tggcgcttca ggaagtacac cgcctgcgaa
     6721 tcctgctcgc cgaagatcgc caccagcacg ttggcaccgg tgacctcctt gttgcccgag
     6781 gactgcacgt gcagcacggc gcgctgcagg acgcgctgga agccgagcgt cggctgggtc
     6841 tcgcgcggat catcggcggc gagcaccggc gtgctctcgc ggatgaagcc ctgcagatca
     6901 ctcttcaact tgtccatgtt ggccccgcag gcgcgcagaa cctcggcggc cgacgggtta
     6961 tcgagcaagg caagcagcag gtgctcgacg gtcatgaact catggcgccg atccctcgcc
     7021 tcgcggaacg cgaggttgat ggtgaactcc agttccttgc tcaacatggt gaacgacctc
     7081 gtcgataagc aaatggggtg gcaatgacgt cctttttcct tgtcaggctt cttccatggt
     7141 gcagagcagc gggtgctggt gggtgcgtga gaacgcgttg acctgggcga ccttggtctc
     7201 ggcgatgtcc cgagtataga caccacatac ccccttcccg cgggtatgga catgcaacat
     7261 gacctgcgtg gcctgctcgc ggttcatgcc gaagaacgct tcgagcacgt gcacgacgaa
     7321 ctccatcggc gtgtagtcgt cgttgagcag caccaccttg tacatgcgcg gcggcttcag
     7381 ttccggcttg gccttctcga ccgccagtcc gccgtcgccg tgccgtgggt tgtgttcctg
     7441 agccatgctg ggatgaaccg atgtctgtcg ttgccggagc gccggtccgt gcggacccac
     7501 gcccctagat atacggaagc ggcacggatt cttccagagc cgtcctgcat tgacctgaga
     7561 caaaaaaagg gcaggcgacc cggaggtcgc ctgccctgtc gtgctgcatc cgcccccgga
     7621 gggaccggat cagtggaact gttcttcttc ggtcgagccc ttcagcgcga cggtggacga
     7681 ctggccgccc tggatcgtct gcgtgacgga gtcgaagtag ccggtgccga cctcgcgctg
     7741 gtgcttgacg gcggtgaagc ccttctcggc ggccttgaac tcggcttcct gcagctcgac
     7801 gaaggcgctc atgttctggc gcgcgtagcc gtaggcgagg ttgaacatcg agtagttcag
     7861 gctgtggaag ccggccagcg tgatgaactg gaacttgtag cccatcgcgc ccagctcgcg
     7921 ctggaacttg gcgatcgtcg cgtcgtcgag gttcttcttc cagttgaacg acggcgagca
     7981 gttgtaggcc agcatcttgc ccgggaactt cgcgtggatc gcctcggcga acttgcgcgc
     8041 gtactcgaga tccggcttgc cggtctcgca ccagaccaga tcggcgaccg gcgcgtaggc
     8101 caggccgcgg ctgatcgcct gctccagacc cggcttgacg cggtagaagc cttcgacggt
     8161 gcgctcgccg gtgcagaacg gcttgtcgtt gtcgtcgaca tcgctggtca gcaggtcagc
     8221 ggcttcggcg tcggtacggg cgacgagcag cgtcggcacg cccatgaggt cggcggccag
     8281 acgcgcggcg gtcagcttct cgacggcttc gcgggtcggc accagcacct tgccgcccat
     8341 gtggccgcac ttcttgaccg aggcgagctg gtcctcgaag tgcacgcccg aggcgccggc
     8401 cttgatcatg gccttcatga gttcgaaggc attcagcacg ccaccgaagc cggcctcggc
     8461 gtcggcgacg atcggctgga agaagtcgat cgagtcgttg ccttcggcat ggtgcagttc
     8521 gtcggcgcgc atcagcgtgt tgttgatgcg ctcgacgacg gccggcaccg agttggccgg
     8581 atacagcgac tggtcgggat acatcgcgcc ggcgaggttg gcatcgcccg cgacctgcca
     8641 gcccgacagg tagatcgcct tcaggccggc cttgacctgc tgcatggcct ggttgccggt
     8701 cagcgcgccc agcgcattga tgaacggctc ggtgttcagg tacttccaga gcttctcggc
     8761 gcccttcgtg gccagcgtgt actcgacgcc ggcagtgccg cgcagacgga caacgtcttc
     8821 ggccgagtag gcacgggtga tgcccttcca gcgcggattt tcggcccagt cgttcttcag
     8881 ctgctcaagg ctcaggtgcg gcatgcgacg gttctccagt aacaagaggt gtggcgcgaa
     8941 acaggcgccg ggatgatgaa cggcgcttca tgtaatttca catcgcgaaa cgtgatcgcg
     9001 taccgagaaa ttgcgcgcag tctaatgtgg ctatgcagca attgacaagg ctcaatcgtc
     9061 aatactaaac attcacgcca tcaatataag acagtcaaca tctgcccgcc gacgatgaat
     9121 tcgccgctgc cccgccacta ctacaaacac aaccggctgc agcagttgcg cggcttctgc
     9181 tatgcggtgc agaccggcag catctcgcgg gcggccgagc gcatgttcct gagccagccg
     9241 tctgtctccc tgcagattca ggcgcttgag cgggagctgg gcgtaacgct gttcgaacgc
     9301 cgcggcccgc gcatccggct cacgcccgag ggccagaccc tctacgagat ggcactgccg
     9361 ctgctcgaag gcctcgatgg cctgcccgag accttcctcg cccggcgcgg cagcgtcgag
     9421 tcgggggagc tcaacatcac ggccggcgaa gccacgatcc tctacctgct gcccgacgtg
     9481 gtgacgcgct tcagcgccgc gcaccccggc atccgcgtgc gctttcacaa tgtgccgggg
     9541 cgcgagggcc tgcggctgct gcgcgaggac gaggttgatt tcgcggtcgg atcgttccgg
     9601 gaagtgccgg acgacatgag ctaccagccg atccacgcct acgaccagac gctgatcacg
     9661 ccgctcgaac acccgctcgc cggccgcctc gaagtgtcgc tcgccgacat cagtccctac
     9721 gaattcatcc tgccgccgcg cggcctgaac acgctgtcga tggtcgaact cgccttccag
     9781 gcgcagaacc tgagcctgaa cgtccggctc gaagccggcg gctgggcggt gatcaagcgc
     9841 tatgtcgaga tgggcctcgg catctcgatc gtctcgggca tctgcctgac gccggacgac
     9901 aagctgtcgt cgctgtcgct gaaccgctat ttcccgcgcc gcacctatgg cgtggcgatg
     9961 cgtcgcggaa aattcctgac cccccaggcc aagcgcttca tcgaattcat cgacccgaac
    10021 ttcttcgcgc cggacaccga tccggccgat gcatgaagag atcaggtgct tcagccgcag
    10081 ccggctttat tcccttccgg tcgcgccagt ccggttccaa tgttgccgca cagcgccaga
    10141 tgcccaggcc tcgatggccg cgaaggtctg ggcaccgcct cgaacctgtg gaagccgccg
    10201 ttgcgcgccc ccca
//
LOCUS       DAOQIE010000126        10202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3320_length_10202_cov_6.236622, whole genome shotgun sequence.
ACCESSION   DAOQIE010000126 DAOQIE010000000
VERSION     DAOQIE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_10202_cov_6.236622"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK144_09325"
     CDS             <1..420
                     /locus_tag="PK144_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65967.1"
                     /translation="LLPGAAAGATAGAARRSRWLSPWACALLLGWALLTTSLQTDRFG
                     LDLSGWHLLALPVAWLAGRLHGRGALGLAPGAAGLELGLSLLGGGADWSAGSPALHLT
                     LYAPAPGTAVIDALVLPLCAHAGARRAGRNSTVPERA"
     gene            complement(463..1137)
                     /locus_tag="PK144_09330"
     CDS             complement(463..1137)
                     /locus_tag="PK144_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF65968.1"
                     /translation="MRAMILAAGRGERMRPLTDLWPKPLLEAGGRSLIEHHLYRLAGA
                     GFTEVVINLGHLGHLIPPRLGDGSRYGLRIDYSPEPPGALETGGGIQHALGLLGEAPF
                     LLINGDIWSEHPLAALRTALAPGDLAHVVLAPNPPYKPQGDFALVGGRVRDDLDPRLT
                     YCGLGVIDPRLFVGHGPGRFPLAPLLTAAMAEDAVSGELHQGDWRDIGTPERLAELDV
                     WLALRR"
     gene            complement(1137..2135)
                     /locus_tag="PK144_09335"
     CDS             complement(1137..2135)
                     /locus_tag="PK144_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNF65969.1"
                     /translation="MNDRLERVRAWAGRTLPGRVLDIAPASADASFRAYFRVRWPGGS
                     AIVMDAPPAHEDCRPFVRIAGALAQLGLNVPRVLAAAIGAGLLLLSDLGDTQYLDKLG
                     DATADRLYGDALDALARLALGGDPASGVLPPYDDALLLREMNLFRDWFLVRHLGLTLD
                     GTEHALLDTTFALLSEKARAQPQVWVHRDYHSRNLMVCAPHDPGVLDFQDAVIGAVTY
                     DLVSLLRDAYIDWPDARVAGWVESYRQRLAGSVLARASADAFLRDFELMGAQRQLKVC
                     GIFARLAWRDGKHRYLADIPRVFEYLLGACARHPELAGLGRFLHARVVPALAGETA"
     gene            2227..4542
                     /gene="lptD"
                     /locus_tag="PK144_09340"
     CDS             2227..4542
                     /gene="lptD"
                     /locus_tag="PK144_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNF65970.1"
                     /translation="MSVTQRSNIITALCLALAATPALAQSGPGTKQKTGPAATQPLRI
                     DDDGRVLGWALCPVDPLANLVTKDTGAPAEGPTTYTADTAESSPEQARLAGDVRVARG
                     DQRLAAPEVVLDRQKNIVHAPKGVQAGTPALGVEAGTGDMNLDTDTADLRDARYYLPQ
                     RNAQGSAGRVVDRRQDQKTDLDEATYSTCQRDAEVWRVRATEMHLDHAAGRGEAWHAK
                     IDIEGVPVLYLPYLSFPLNNDRQSGFLAPKLGFDSTSGLDLAVPYYWNIAPNYDATIT
                     PRYIAERGLLLGTEFRFLTGDAKGITSFEFLPNDRDRDGRDRGSFHFFGSGHYGPFSS
                     TLLYDWASDDNYFRDLGNGLDITDTDYLERRLDTYVVNPFGNLLIRFRGYQTLNGEDF
                     TRPKQYAQLPQVAFWNTWIVPNQVSFELHSEATRFTHPDQVEGSRFFIEPALSLPLQT
                     TWGFLTPRLALNATAYQLDYRRDQPDDYRDSPTRVAPVASLDTGLIFDRPLTISGTGF
                     TQTLEPRLYYLRVPYRRQTDIPLFDTTTYSESYDWLFYDNRFAGIDRLGDANQLTTAI
                     SSRLLGASDGVERLRVSVGQIQYFSDRKVQLGADSPTLTNNSDLVAEATLRLDQNWYA
                     RAATLWDTTESGSRRTAYDLRYHDADGRILNVAYRYANGVPDPISGQTSLEQIDLAGH
                     WPINPRWRVFGRYNYSLLYSHTTDTFAGFEYGDCCWAVRLLGRQYREYPQDEDPKNAV
                     MLEIELKGLTSVGASIDRFLDKSIIGYQRAD"
     gene            4571..5914
                     /locus_tag="PK144_09345"
     CDS             4571..5914
                     /locus_tag="PK144_09345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF65971.1"
                     /translation="MKPLLLALTLALALPTTVIAQELRFGPSTPAAGGAGRELDSVAA
                     VVDDDVITRAELDQAVQTALGQLRARGGQLPPMDVVRKQVLERQILLKLQERAAARNG
                     ISVDDLTLNAAIATVAQRNGVSVDELRRRVERDGFSFARYREEIRRELVSSRLRQKVV
                     DPSITVSDQEVDLALGIAPGAGPGAAAAPAREYRLAHILVGLPEGADAAQIEAAQAKA
                     DALVAELRKGADFARLATSVSDAGDALNGGEIGWRSAAQLPPAFAEAIGSMSAGQVTA
                     PLRGTNGFEIVRVIEVRNAGEAAPGQIAQTEVRHILLRAAPGESDDAVRQRALRLRAG
                     LNDAGFAAAARAQSQDPGSAPKGGELGWVSPGQLVPEFERAMAALKPGEISQPVKTSF
                     GWHLIEVKNRRQVAGKVEDARRMQAREAIYKRKIEEEWDIWLRRLRDEAYVDIRL"
     gene            5940..6956
                     /gene="pdxA"
                     /locus_tag="PK144_09350"
     CDS             5940..6956
                     /gene="pdxA"
                     /locus_tag="PK144_09350"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNF65972.1"
                     /translation="MSDSVLPRLIVTAGEPAGIGPELCVRLAQAPWPCALVVAGNREL
                     LAHLAGVLGLPLTTVAWDPAAAPRPHEPGRLTVLDLPLAAEVIPGRADPANAAHVLAM
                     LAAACDGTRDGHFDALVTGPVHKGVINDAGTPFSGHTEFFAERLGGARPVMMLAAGTL
                     RVALATTHLPLRAVADAITRESLTEVLTILDHDLRTRFAIAAPRILVCGLNPHAGEGG
                     HLGREELDVIEPVLAELRAAGLDLSGPLPADTLFQPQRLAHADAVLAMYHDQGLPVLK
                     YAGFGQAVNITLGLPVIRTSVDHGTAFDLAGSGRASAASLQAALACALDLVERRRRAA
                     GSPA"
     gene            6953..7750
                     /gene="rsmA"
                     /locus_tag="PK144_09355"
     CDS             6953..7750
                     /gene="rsmA"
                     /locus_tag="PK144_09355"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNF65973.1"
                     /translation="MNGPTGAHQARKRFGQHFLHDPHVIARIIAAVAPLPGEHLVEIG
                     PGLGAITVPLLRACGQLSAVELDRDVIPHLRAATAGLGRLDILEADALTVDFSALRHD
                     DRPLRLVGNLPYNISTPLLFHFLEAAAHIRDMHFMLQKEVVERMAAGPGDADYGRLSV
                     MLQARLKVVPLFEIGPGAFRPPPKVDSAIVRLVPLPEPRVPAALQAAFAEVVRLAFAQ
                     RRKQLRNTLKTVLAADAIEAAGVDPAARAETLDLAAYVRLAEAIPPR"
     gene            7783..8901
                     /gene="tal"
                     /locus_tag="PK144_09360"
     CDS             7783..8901
                     /gene="tal"
                     /locus_tag="PK144_09360"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNF65974.1"
                     /translation="MNNIDDHPLRRLNAAGQSPWYDNIHRSMLEGGALARLIRDDDLR
                     GVTSNPAIFEKAIAGTDEYDDAIRQALAARPGLPARELFFLLALDDIRAAADVLLPVH
                     RATDGVDGFVSMEVSPDLAHDTEATIREAHELAASLDRPNVMIKVPATRAGIPAIEEL
                     IADGIPVNVTLLFAVGRYAEVAEAYLRGLERRLDHGQPLARIASVASFFVSRVDSALD
                     PLLATQAPALAGTLAIANARLARARFHALFDGPRFARLAAAGARPQRLLWASTGTKNP
                     AYSDVLYVETLIGSDTVNTMPPATWTAFRDHGRVAATLDTGLEAAEAAVAQLAGLGID
                     LATVTERLEAEGVAAFLKSFETLLAALDAKARAVASAA"
     gene            8927..9319
                     /gene="apaG"
                     /locus_tag="PK144_09365"
     CDS             8927..9319
                     /gene="apaG"
                     /locus_tag="PK144_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNF65975.1"
                     /translation="MKPTDAPALHDIRISVETEYIAAQSEPARDRYAFAYTVTIENRG
                     GLPAKLLTRHWIITDAGGRTIEVRGDGVIGEQPYLRPGEGFRYTSGTVIDTPVGRMHG
                     SYHLIDDDGTEFDAPIPPFVLSMPRTLH"
     gene            9335..9931
                     /locus_tag="PK144_09370"
     CDS             9335..9931
                     /locus_tag="PK144_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF65976.1"
                     /translation="MSLRTSYTLLAPIYDRMVAAATQAARRASLARLPADARQVLLVG
                     AGTGLDFPLLPAGPQYTAIDLTPAMLDRARQRAAARPEVAIELRSGDALALDFADASF
                     DAVILHLIVAVVPDAARALAESARVLRPGGRVLLYDKFLRPGQSAPLRRLLSPLLGRI
                     ASRTDVVLEHHLPPTLRVLEDEASLAGGWFRRVLLERV"
BASE COUNT         1511 a         3861 c         3431 g         1399 t
ORIGIN      
        1 ctgctgcccg gggccgcggc cggtgcgacg gccggcgccg cgcgccgctc gcgctggctg
       61 tcgccgtggg cctgcgcgct gctgctcggc tgggcgctgc tgacgacgag tctgcagacc
      121 gatcgcttcg ggctcgacct cagcggctgg catctgctcg cgctgccggt cgcatggctc
      181 gcgggccggc tgcacgggcg cggcgcgctc gggctcgcgc ccggcgccgc cgggctcgag
      241 ctcggtctgt cgctgctcgg cggcggcgcg gactggtcgg ccggatcgcc ggcgctgcac
      301 ctgacgctgt acgccccggc gcccggcacc gccgtgatcg atgcgctcgt gctgccgctg
      361 tgcgcgcacg ccggcgcgcg ccgcgcgggg cgcaattcaa ccgtgccgga gcgagcctga
      421 cccgctcttg tgtcttgaca gcccgggtgg gctggatcgg cctcagcggc gcagcgccag
      481 ccacacgtcg agttcggcca gccgctcggg cgtgccgatg tcgcgccagt cgccctggtg
      541 cagttcgccg ctgaccgcgt cctcggccat cgccgcggtc agcagcggcg ccagcggaaa
      601 gcgccccggg ccgtggccga cgaacagccg cggatcgatg acgccgaggc cgcagtaggt
      661 caggcgcgga tcgagatcgt cgcgcacgcg gccgccgacg agtgcgaagt cgccctgcgg
      721 cttgtacggc gggttcggtg ccagcacgac gtgcgcgaga tcgccggggg cgagcgccgt
      781 gcgcagggcg gcgagcgggt gctcgctcca gatgtcgccg ttgatcagca ggaacggcgc
      841 ctcgccgagc aggccgagcg cgtgctggat gccgccgccg gtttccagcg cgccgggcgg
      901 ttccggcgaa tagtcgatgc gcaggccgta gcggctgccg tcgccgagcc gcggcgggat
      961 caggtggccg aggtggccga ggttgatgac gacctcggtg aagcccgcgc cggcgaggcg
     1021 gtacagatga tgctcgatca gcgagcgccc accggcttcg agcaggggct tcggccagag
     1081 gtcggtcagc ggccgcatgc gttcgccgcg cccggcggcg aggatcatcg cgcgcatcag
     1141 gcggtctccc cggccagcgc cggcaccacg cgcgcgtgca ggaagcgccc gagcccggcg
     1201 agttccgggt ggcgtgcgca ggcgccgagc aggtactcga acacgcgcgg gatgtcggcg
     1261 agatagcggt gcttgccgtc gcgccaggcc agccgcgcga agatgccgca gaccttgagc
     1321 tggcgctgcg cgcccatcag ctcgaaatcg cgcaggaagg catcggcact cgccctcgcc
     1381 agcacactgc cggcgaggcg ctggcggtag gactcgaccc agccggcgac gcgcgcatcc
     1441 ggccagtcga tgtaggcatc gcgcagcagc gagacgaggt cgtaggtgac ggcgccgatc
     1501 acggcatcct ggaagtcgag cacgccggga tcgtgcggcg cgcagaccat caggttgcgc
     1561 gagtgatagt cgcgatgcac ccagacctgc ggctgcgcac gcgccttctc cgacagcagc
     1621 gcaaacgtcg tgtcgagcag cgcgtgctcg gtgccatcga gcgtgagtcc cagatgccgg
     1681 accaggaacc agtcgcggaa caggttcatc tcgcgcagca gcagggcatc gtcatacggc
     1741 ggcagcacgc cgctcgccgg atcgccgccg agcgcgaggc gcgccagcgc atcgagcgcg
     1801 tcgccgtaga ggcggtcggc ggtcgcatca ccgagcttgt cgagatactg cgtgtcgccg
     1861 agatcactga gcagcagcag gccggcgccg atggcggcgg cgagcacgcg cggcacgttc
     1921 aggccgagct gcgccagcgc gccggcgatg cgcacgaacg gccggcagtc ctcgtgcgcg
     1981 ggcggcgcgt ccatgacgat cgccgagccg cccggccagc gcacacggaa gtaggcacgg
     2041 aagctcgcat cggcggaggc cggtgcgatg tcgagcacgc ggcccggcag ggtccggccg
     2101 gcccaggccc ggacgcgttc cagtcgatcg ttcacgtggg gggttcctcg ggcgcgtggg
     2161 ggagcgccga aattgaagct gcaaggggtt tcgtgcgatg gttacgcgct tccagaagct
     2221 gcgtccatgt ccgtgaccca acgctccaac atcataacag cgctctgtct ggcgctggcc
     2281 gccaccccgg cgctggcgca gtccggaccc ggcaccaagc agaagaccgg ccccgccgcg
     2341 acgcagccgc tgcgcatcga cgatgacggc cgcgtgctcg gctgggcgct gtgcccggtc
     2401 gatccgctcg cgaatctggt cacgaaggac accggcgccc cggccgaggg cccgaccacc
     2461 tataccgccg acaccgccga gagcagcccc gaacaggcgc gccttgccgg cgacgtgcgc
     2521 gtcgcccgcg gcgaccagcg gctcgcagcc cccgaggtcg tgctcgatcg ccagaagaac
     2581 atcgtgcatg cgccgaaggg cgtgcaggcc ggcacgccgg cgctcggcgt agaggccggg
     2641 accggcgaca tgaacctcga taccgacacc gccgacctgc gcgacgcacg ctactacctg
     2701 ccgcagcgca acgcccaggg ttcggccgga cgcgtcgtcg accgccgcca ggaccagaag
     2761 accgatctcg acgaagccac ctactcgacc tgccagcgcg acgccgaggt ctggcgcgtg
     2821 cgcgcgaccg agatgcacct cgatcatgcc gccggccgcg gcgaggcctg gcacgcgaag
     2881 atcgacatcg aaggcgtgcc ggtgctgtac ctgccgtatc tgtcgttccc gctgaacaac
     2941 gaccgccagt cgggcttcct cgcgccgaag ctcggctttg acagcaccag cggcctcgac
     3001 ctggccgtgc cgtactactg gaacatcgcg ccgaactacg acgcgaccat cacgccgcgc
     3061 tacatcgccg agcgcggcct gctgctcggt accgagttcc gcttcctgac cggtgacgcc
     3121 aagggcatca ccagcttcga gttcctgccg aatgaccggg accgggacgg ccgcgaccgc
     3181 ggctccttcc acttcttcgg cagcggccat tacggcccgt tctcgagcac gctgctgtac
     3241 gactgggcct cggacgacaa ctacttccgc gatctcggca acggcctcga catcaccgac
     3301 accgattacc tcgaacgccg gctcgacacc tacgtcgtca acccgttcgg caacctgctg
     3361 atccgcttcc gcggctacca gacgctgaac ggcgaggact tcacccgccc gaagcagtac
     3421 gcgcagctgc cgcaggtggc gttctggaac acctggatcg tgccgaacca ggtgagcttc
     3481 gagctgcaca gcgaggcgac ccgcttcacg catccggacc aggtcgaggg ctcgcgcttc
     3541 ttcatcgagc cggcgctcag cctgccgctg cagaccacct ggggcttcct gaccccgcgc
     3601 ctggcactga acgccaccgc ctaccagctc gactaccgcc gcgaccagcc cgatgactac
     3661 cgcgacagcc cgacgcgcgt cgcgccggtc gcgagcctcg acaccggcct gatcttcgat
     3721 cggccgctga cgatctccgg caccggcttc acgcagacgc tggagccgcg cctgtactac
     3781 ctgcgcgtgc cctaccgccg ccagaccgac atcccgctgt tcgacacgac cacgtacagc
     3841 gagtcctacg actggctgtt ctacgacaac cgcttcgccg gcatcgaccg cctcggcgat
     3901 gccaaccagc tgaccacggc gatcagctcg cggctgctcg gcgccagcga cggcgtcgag
     3961 cggctgcgcg tgtcggtcgg ccagatccag tacttctccg atcgcaaggt gcagctcggg
     4021 gccgacagcc cgacgctcac caacaactcc gatctggtgg ccgaagccac gctgcgcctg
     4081 gaccagaact ggtacgcccg cgccgccacg ctgtgggaca ccaccgaatc cggctcgcgg
     4141 cgcaccgcct acgacctgcg ctaccacgac gccgacggcc gcatcctgaa cgtcgcctac
     4201 cgctacgcca acggcgtgcc cgatccgatc tccggccaga ccagcctcga acagatcgac
     4261 ctcgccggcc actggccgat caacccgcgc tggcgcgtgt tcggccgcta caactattcg
     4321 ctgctgtaca gccacacgac cgataccttc gccggcttcg agtacggcga ctgctgctgg
     4381 gccgtgcgcc tgctcggccg tcagtaccgc gagtatccgc aggatgaaga cccgaagaat
     4441 gccgtcatgc tcgaaatcga gctgaagggg ctgacctcgg tcggtgccag catcgaccgc
     4501 ttcctcgaca aatccatcat cggctaccag cgtgccgact gagccgcccg tacggacccg
     4561 tccacccgcc atgaaaccgc tgctgctcgc cctgaccctg gccctggccc tgcccacgac
     4621 cgtcatcgcg caggaactgc gcttcggacc gtcgacccct gccgccggcg gtgcgggccg
     4681 tgaactcgat tcggtggcgg ccgtcgtcga tgacgacgtg atcacgcgcg ccgaactcga
     4741 tcaggcggtg cagaccgcac tcggccagct gcgcgcgcgc ggcggccagc tgccgccgat
     4801 ggacgtggtc cgcaagcagg tgctggagcg ccagatcctg ctgaagctgc aggagcgcgc
     4861 cgcggcgcgc aacggcatca gcgtcgatga cctgacgctg aatgccgcga tcgccaccgt
     4921 cgcgcagcgc aacggcgtca gcgtcgacga gctgcgccgg cgcgtcgagc gcgacggctt
     4981 cagcttcgcg cgctaccgcg aggaaatccg ccgcgaactg gtgtcctcgc ggctgcgcca
     5041 gaaggtcgtc gacccgagca tcaccgtctc cgatcaggag gtcgacctcg cgctcggcat
     5101 cgctcccggt gccggccccg gcgccgctgc ggccccggcg cgcgagtacc ggctcgcgca
     5161 catcctcgtc ggcctgcccg aaggcgccga cgccgcgcag atcgaagccg cgcaggccaa
     5221 ggccgatgcg ctggtcgcgg aactgcgcaa gggcgccgac ttcgcacgcc tcgcgacctc
     5281 ggtgtccgat gccggcgatg cgctcaacgg cggcgagatc ggctggcgct cggccgcgca
     5341 gctgccgccg gccttcgccg aggcgatcgg ctcgatgtcg gccgggcagg tgacggcgcc
     5401 gctgcgcggc accaatggct tcgagatcgt gcgcgtgatc gaggtgcgca acgccggcga
     5461 ggcagcaccc gggcagatcg cgcagaccga ggtccgccac atcctgctgc gcgccgcccc
     5521 cggcgagagc gatgacgccg tgcgccagcg cgcgctgcgc ctgcgcgccg gcctcaacga
     5581 cgcgggcttc gccgctgcgg cgcgcgccca gtcgcaggac cccggcagcg ccccgaaggg
     5641 cggcgagctc ggctgggtca gcccgggcca gctggtgccg gagttcgagc gggcgatggc
     5701 ggccctgaag cccggcgaga tcagccagcc ggtcaagacc tcgttcggct ggcatctgat
     5761 cgaggtcaag aaccgccgtc aggtcgccgg caaggtcgag gacgcccgcc gcatgcaggc
     5821 gcgcgaggcc atctacaagc gcaagatcga agaggaatgg gatatctggc tgcggcggct
     5881 gcgcgacgaa gcctacgtcg acatccgcct ctgacgcgcg ccgccccggt ccgcgccgca
     5941 tgtccgattc cgtgctgccc cgcctgatcg tcaccgccgg cgaaccggcc ggcatcgggc
     6001 cggaactctg cgtgcgtctg gcgcaggcgc cgtggccgtg cgcgctggtg gtggccggca
     6061 accgcgaact gctcgcgcat ctggccggcg tgctcggcct gccgctcacc accgtcgcct
     6121 gggatccggc cgctgccccc cggccgcacg aacccggccg gctgacggtg ctcgacctgc
     6181 cgctcgcagc tgaagtcatc cccggccggg ccgatccggc caacgccgcg cacgtgctcg
     6241 cgatgctggc ggcggcctgc gacggcaccc gcgacggaca cttcgatgcg ctggtcaccg
     6301 gcccggtgca caagggcgtc atcaacgatg ccggcacgcc gttcagcggc cacaccgaat
     6361 tcttcgccga acgcctcggt ggcgcgcggc cggtgatgat gctcgctgcc ggcacgctgc
     6421 gcgtggcgct cgcgaccacg cacctgccgc tgcgtgcggt ggccgatgcg atcacgcgcg
     6481 aaagcctcac cgaagtgctg acgatcctcg atcacgacct gcgcacgcgc ttcgcgatcg
     6541 ccgcgccgcg catcctggtc tgcggcctga acccgcacgc cggcgagggc ggacacctcg
     6601 ggcgcgagga gctcgacgtg atcgaacccg tgctcgccga gctgcgcgcc gccggtctcg
     6661 atctgtccgg gccgctgccg gccgacaccc tgttccagcc gcagcgcctc gcgcacgccg
     6721 acgcggtgct ggcgatgtat cacgatcagg gcctgccggt gctgaagtac gcgggcttcg
     6781 ggcaggcggt caacatcacg ctcggcctgc cggtgatccg cacctcggtc gatcacggca
     6841 cggccttcga tctggccggc agcggtcgcg cctcggccgc cagcctgcag gccgcactcg
     6901 cctgcgcgct cgatctggtc gaacgccgcc gccgcgccgc cggatcgccg gcgtgaacgg
     6961 accgaccggc gcgcatcagg cgcgcaagcg cttcggccag cacttcctgc acgatccgca
     7021 cgtgatcgcg cgcatcatcg ccgcggtcgc gccgctgcca ggcgagcatc tggtcgagat
     7081 cggccccggg ctcggtgcga tcaccgtgcc gctgctgcgc gcctgcgggc agttgagcgc
     7141 ggtcgaactc gatcgcgacg tgatcccgca cctgcgcgcc gccaccgccg gcctcggcag
     7201 gctcgacatc ctcgaagccg atgcgctgac ggtcgatttc tctgcactgc gccacgacga
     7261 ccgccctctg cggctggtcg gaaacctgcc atacaacatc tcgacgccgc tgctgtttca
     7321 cttcctcgaa gccgcggcgc acatccgcga catgcacttc atgctgcaga aggaagtcgt
     7381 cgagcgcatg gctgccggcc ccggcgatgc cgactacggc cggctgtcgg tgatgctgca
     7441 ggcgcgcctg aaggtcgtgc cgctgttcga gatcggcccc ggtgccttcc ggccgccacc
     7501 gaaggtcgat tcggcgatcg tgcgcctcgt gccgctgccc gagccgcgcg tgccggcggc
     7561 cctgcaggcg gccttcgccg aggtcgtgcg gctggccttc gcgcagcggc gcaagcagtt
     7621 gcgcaacacg ctgaagaccg tgctcgccgc ggacgcgatc gaggccgccg gcgtcgatcc
     7681 ggccgcgcgc gccgaaacgc tggatctggc cgcctacgtg cgtctggccg aagccatacc
     7741 tccgcgctga tccggcggcg cggtccacca gggagaacga tcatgaacaa catcgacgac
     7801 cacccgctgc gccgcctcaa cgccgccggc cagagcccgt ggtacgacaa catccaccgc
     7861 agcatgctcg aaggcggcgc cctcgcccgc ctgatccgcg acgacgacct gcgcggcgtc
     7921 acctcgaacc cggcgatctt cgagaaggcc atcgccggca ccgacgaata tgacgatgcc
     7981 atccgccagg ccctcgccgc ccgccccggg ctgccggcgc gcgagctgtt cttcctgctc
     8041 gcgctcgatg acatccgtgc cgccgccgac gtgctgctgc cggtgcaccg cgccaccgac
     8101 ggcgtcgacg gcttcgtcag catggaggtc tcgcccgacc tggcgcacga caccgaggcc
     8161 accatccgcg aggcgcacga actcgccgcc agcctcgacc ggccgaacgt catgatcaag
     8221 gtgccggcca cgcgcgccgg catcccggcg atcgaggaac tgatcgccga cggcatcccc
     8281 gtcaacgtca cgctgctgtt cgcggtcggg cgctacgccg aggtcgccga agcctatctg
     8341 cgcgggctgg agcggcggct cgatcacggc cagccgctgg cgcgcatcgc ctcggtcgcg
     8401 agcttcttcg tgtcgcgcgt cgatagcgcg ctcgatccgc tgctcgccac ccaggccccg
     8461 gcgctggccg gcacgctggc gatcgccaac gcccggctgg cgcgggcgcg cttccatgcg
     8521 ctgttcgacg gcccgcgttt cgcgcggctc gccgctgccg gcgcccgtcc gcagcgcctg
     8581 ctgtgggcga gcaccggcac caagaatccg gcctacagcg acgtgctcta cgtcgagacc
     8641 ctgatcggca gcgataccgt caacaccatg ccgccggcga cctggacagc gttccgcgac
     8701 catggccgcg tcgcggccac gctcgacacc ggcctcgaag ccgccgaagc cgcggtcgcg
     8761 caactcgccg gactcggcat cgaccttgct acggtcaccg aacgcctcga agccgaaggc
     8821 gtcgccgcct tcctgaaatc cttcgagacc ctgctggctg cgctcgacgc caaggcgcgc
     8881 gccgtcgcca gtgccgcctg acccgccccg cccgagagcc tcccccatga agccgaccga
     8941 cgcgcccgcc ctgcacgaca tccgcatctc ggtcgagacc gaatacatcg cggcgcaatc
     9001 ggaaccggcc cgcgaccgct acgccttcgc ctacaccgtc accatcgaga accgcggcgg
     9061 cctgccggcg aagctgctga cccggcactg gatcatcacc gatgccggcg gacgcacgat
     9121 cgaggtgcgc ggcgacggcg tgatcggcga acagccgtat ctgcgccccg gcgaaggctt
     9181 ccgctacacc agcggcaccg tcatcgacac cccggtcggg cgcatgcacg gcagctatca
     9241 cctgatcgat gacgacggca ccgaattcga cgcgccgatc ccgcccttcg tgctgtcgat
     9301 gccgcgcacg ctgcactgaa gccgtgcacg gccgatgagc ctgcgcacga gctacacgct
     9361 gctcgcgccg atctacgacc gcatggtcgc agcggccacg caggcggcgc ggcgcgcgag
     9421 cctcgcgcgc ctgccggccg atgcccggca ggtgctgctg gtcggggccg gcaccgggct
     9481 ggatttcccg ctgctgccgg ccggtccgca gtacacggcg atcgacctga ccccggcgat
     9541 gctcgatcgc gcccgccagc gcgccgcggc gcggccggaa gtcgcgatcg aactgcgcag
     9601 cggcgatgcg ctggcgctcg atttcgccga tgcgagcttc gatgccgtga tcctgcacct
     9661 gatcgtcgcc gtcgtgcccg acgccgcgcg cgcgctcgcc gaatccgcac gcgtgctgcg
     9721 ccccggcggc cgcgtgctgc tgtacgacaa gttcctgcgt cccggccagt ccgccccgct
     9781 gcgccggctg ctcagcccgc tgctcggccg catcgccagc cgtaccgatg tcgtgctcga
     9841 acatcacctg ccaccgacgc tgcgcgtgct cgaagacgaa gcctcgctgg ccggcggctg
     9901 gttccggcgc gtgttgctgg aacgggtctg atcgcggacc cagggcctga aggcgggttc
     9961 cggcgcaggc caggccgtgt gcaggacatt gaccatcagc agaccgcgcc tgacccgccc
    10021 tgcgtccggg ctgacagcgt gcggccgatg cccgcgtgca tgggaatgtc ttgcggccgg
    10081 ggcgcgcagc cttgcgaatg ccccccgaaa gcctttcacc cgcggtgcaa aggcttccac
    10141 ctgcacacca aagcctttga cccgcactcc aaagcctttg acccacactc caaagccttt
    10201 ga
//
LOCUS       DAOQIE010000127        10138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3347_length_10138_cov_8.425964, whole genome shotgun sequence.
ACCESSION   DAOQIE010000127 DAOQIE010000000
VERSION     DAOQIE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3347_length_10138_cov_8.425964"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..386)
                     /locus_tag="PK144_09375"
     CDS             complement(3..386)
                     /locus_tag="PK144_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HNF65977.1"
                     /translation="MSLKKTTPDSAKLDRLVAEARKAREQREAGYREQALKLYPWICG
                     RCAREFTRVNLHELTVHHRDHNHDNNPPDGSNWELLCLYCHDNEHSRHADLVRGADYS
                     SAAPAPAATFKAFGALADLLKKQDG"
     gene            546..1646
                     /locus_tag="PK144_09380"
     CDS             546..1646
                     /locus_tag="PK144_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF65978.1"
                     /translation="MPDPSPAPFPESRPLLDRTLVVRTLGLALLALLIAGCALTLLPF
                     LTAILWGLILTVSFAPLHAGLTRRIGRRLATTVIVALPVLAVLLPLFLFADGIASNFM
                     VLTAKAEALLSGNSPPSAPEWLNDIPLFGHDLHDWLNEVLHDEVALTEQLRKLIAPTR
                     DFLLLALRKLGAGLLDLTLSVIVMGVLLNDLHRIGVRTRQFLAQFAGTRAAELLAVAS
                     ATVRGVVAGLLGTALAQALLAGIGFAIAGTGSAVFYGFVTFFLSFVPFGPVFVWGPLA
                     WQLYGAGSTGMAIFLAVWGAAVVGTIDNILKPLLIGRGLELPLLLVFLGVLGGALSFG
                     LIGVFVGPVLLAVGQNLARLWFVEGVARRPEG"
     gene            complement(1884..3500)
                     /locus_tag="PK144_09385"
     CDS             complement(1884..3500)
                     /locus_tag="PK144_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNF65979.1"
                     /translation="MNPQNPQNPQDPNLPQSPQASQNPQTPPAATVPPVARFARARHG
                     AGHRRGLALESTGLRRSLLAAALVAAFGAGAVLHGGSELIEQAHAEAPAVTPAPARIV
                     TPDFGALVERYGPAVVNIQVEGTVRTAAQQPQIQIDPNDPLYDFLRRFGLPGLRGRGG
                     PAPDVVRGEGSGFIVRADGVILTNTHVVDGAEHVTVRLTDRREFKARVLGKDDQSDIA
                     VLKIDASDLPTVRIGDPARSRVGDWVLAIGSPFGFENSVSAGIVSAKARTLPDDNYVP
                     FLQTDVAVNPGNSGGPLFNADGEVIGVNSQIYSRTGGYQGISFAIPIDVAMKVEQQIV
                     AHGKVTRGRLGVAIQDVSAPLAAAFGLKTPHGALVSSVEPGSAGEAAGLEAGDVIVAL
                     DGQTIERSADLPPRIADSKPGSHVELTVIRKGEEKTLKATVGEFRAAAREAGDEAAPG
                     QGRLGLALRPLSPQEKRGNDLDSGLLVQQASGPAARAGVAPGDVVLAINGEPVNTVAQ
                     LKERLAHAGKQIALLIQRGEARIFVPVDLG"
     gene            3655..4923
                     /locus_tag="PK144_09390"
     CDS             3655..4923
                     /locus_tag="PK144_09390"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNF65980.1"
                     /translation="MEIHLVGGAVRDALLGRSVGERDWVVTGATPDELLALGYKRVGK
                     DFPVFLHPETGEEYALARTERKTAPGYHGFAIDAAPHVTLEDDLARRDLTVNAIAQAA
                     DGRLIDPFGGRRDLEARVLRHVSPAFAEDPVRILRLARFAARYAPLGFTVAPETQALM
                     RAMVAAGEVDALQPERVWQELARALGEPVPGRFIEVLRDCGALARLLPELDRLWGVPQ
                     PPQHHPEIDTGVHVLLVLEQAVRLGADVPTRYAALLHDLGKGTTPPAEWPKHTAHEAR
                     GAALAREVSERLRAPKEFRELAVLVAELHTRAHRALELRTTTLLETLERLDALRRPER
                     FARWLLACEADARGRTGLETRPYPQAAWLAQAREAAAAVPVQPLVERGLKGPEFGEAL
                     RLARLDALRAARKRRAAAADAAGEGSAATS"
     gene            5003..5221
                     /locus_tag="PK144_09395"
     CDS             5003..5221
                     /locus_tag="PK144_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65981.1"
                     /translation="MNVGNIGSSSIEQFISNQRPPSQSEGAPQQRHVEAERQQASEAL
                     VRSAPAAPKDIPAFDPERRIGAYIDVTA"
     gene            5598..7142
                     /locus_tag="PK144_09400"
     CDS             5598..7142
                     /locus_tag="PK144_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF65982.1"
                     /translation="MRTLACSALVLVTGLMPVAVVHAAPFPAGPLGSSSQSTYLLPSM
                     TGVALTPLLTVGDAAGNGYRMAGIPDGLGAFDNGDGSFTLLMNHELGASAGAVHAHGA
                     TGAFVSEWVIDKSTLTVLSGRDQIQTVNLLNTSTGQYQPAINATLTRLCSADLPAPTA
                     FFNPGSGLGTSTRFFMNGEETSGGRAFVHTVTGADAGQSYEFAALGHAAWENVVARPT
                     ADDRTVVLGTDDTHPNGDAANAKGRVYVYFGDKQSTGTDLEKAGLTNGTTWAIKVNGA
                     AIEDRSAAANFGLDADGKASFSLVDDGSGTNFLRPEDITWDTQNPDRAYFVTTDGLDD
                     ADPASRTSRLWRITLNDVNDPAAGGVIELLIDGRQPGQPQMMDNLTVAADGTVLIQED
                     VGNTARLGKVWRYDPNTKTTVEVAAHDPARFLLGAPGFLTQDEESSGIIDVTALFDGV
                     AGYDTGTYQYFLLDVQAHYALTDPALVEGGQLLLMAVQAVPEPGSLSLMAAMLGFTLY
                     GARRKR"
     gene            7474..9144
                     /locus_tag="PK144_09405"
     CDS             7474..9144
                     /locus_tag="PK144_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNF65983.1"
                     /translation="MARLAAEDDAEARGAVFTRIEVVEFILDLVGYTPDRPLHACRLL
                     EPAAGCGEFLRVAVRRLLAAWRAAGSPGDTDILAVLGPALQAVELHHDSCRRARAALG
                     EELAAAGIGADTAQALIRQWLRQGDFLLSALPASFDFVVGNPPYVRQELIPPVLLAEY
                     RRRYATLYDRADLYVPFIERALSLLAAGGRLGFICPDRWMKNRYGGPLRRLVAEGFHL
                     QAVIDMDETPAFHSEVAAYPAIVVIARARPGRTRIAHRPPLDAGVLGPLAAALQAAAK
                     PDPSLPEVREQQLVCLRGSDAPWLLDTDARTNLIRRIEAGFPLLEATGCRVGIGVATG
                     ADRVFIGDYASLDVEPQRKLPLAMTADLESGELRWRGLGVVNPFGDDGRLVDLRDWPR
                     LRAYLERHRDVIAARHCARKSPGNWYRTIDRIHPALAAEPKLLIPDIKGSAHVVHEPG
                     RLYPHHNLYYVTATAWDLHALQAVLLSGLTRAFIASYSTRMRGGYLRFQAQYLRRLRL
                     PYWHELAGPLRQELIHAARSRDLAACDRAVAQLYGLSAAEQAELLAQR"
     gene            9145..9891
                     /locus_tag="PK144_09410"
     CDS             9145..9891
                     /locus_tag="PK144_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017173568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HNF65984.1"
                     /translation="MALDLADYELRATAAVQAFWNSRETARQRQQAAGLLDAGERAGV
                     TAGRNMDGFCGLIVALAHANGLDAACIHRARRLLTLPGYFRPTKQWDLLIVDPHDGAL
                     IAAIELKSQVGPSFGNNFNNRTEEAIGTAHDFWTAYREGALGATLRPFTGWLMLVEDA
                     PGSRRPVRDASPHFRVLPEFAAASYLRRYELLCERLVREQLYTAAAVIASPRSAAGDG
                     QWTELSPATGLRTFVSALAGHMAAVAAQRG"
     gene            9872..>10138
                     /locus_tag="PK144_09415"
     CDS             9872..>10138
                     /locus_tag="PK144_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65985.1"
                     /translation="MPRSAAETDQARSDHVFSQQAGGIRLNLSGRMAHRSGGAPLLLL
                     HARCRFGGWQKQAVCTRTARLSAQRDLIAAAGDLRRDVAVAIGLC"
BASE COUNT         1381 a         3651 c         3543 g         1563 t
ORIGIN      
        1 cctcagccgt cctgcttctt cagcagatcg gccagtgcgc cgaaagcctt gaatgtcgca
       61 gccggcgccg gtgcggcgct ggagtagtcg gcgccgcgca ccagatcggc gtggcgcgag
      121 tgctcgttgt cgtggcagta caggcacagc agctcccagt tgctgccgtc cggcgggttg
      181 ttgtcgtggt tgtggtcgcg gtggtgcacg gtcagctcgt gcaggttgac gcgcgtgaac
      241 tcgcgcgcgc agcgcccgca gatccacgga tacagcttca gcgcctgttc gcggtaaccg
      301 gcctcgcgct gctcgcgggc cttgcgtgcc tcggcgacca gacggtcgag cttggcgctg
      361 tccggggtgg ttttcttcag gctcatggca gcggcgcctc cggcgcggac ggcaggggtt
      421 acgagggggg ctgacagcag ccgcgatcgt agcgcgcgcc ggccgtgctc gccggccgtg
      481 ctcgccgagc gtcccggcca gccgttacgc tgaccgctct cccgctgact gccggactcg
      541 cgtcgatgcc cgatccttcg cccgcacctt tcccggaatc gcgcccgctg ctcgatcgca
      601 cgctggtcgt gcgcacgctc ggcctcgcgc tgctcgcgct gctcatcgcc ggctgcgcgc
      661 tgacgctgct gccgttcctg accgcgatcc tctggggact gatcctgacc gtgtcgtttg
      721 cgccgctgca cgcggggctc acgcggcgca tcggccggcg gctggcgacg acggtgatcg
      781 tggcgctgcc ggtgctggcg gtgctgctgc cgctgttcct gttcgccgac ggcatcgcca
      841 gcaacttcat ggtgctgacc gcgaaggccg aggcgctgct gtccggcaac tcgccgccga
      901 gcgcgccgga atggctcaac gacatcccgc tgttcggtca cgacctgcac gactggctga
      961 acgaggtgct gcacgacgag gtggcgctga ccgagcagct gcgcaagctg atcgcgccga
     1021 cgcgcgattt cctgctgctg gcgctgcgca agctcggcgc cgggctgctc gacctgacgc
     1081 tgtcggtgat cgtcatgggc gtgctgctga acgacctgca ccgcatcggc gtgcgcacgc
     1141 gccagttcct cgcgcagttt gccggcacgc gcgcggccga gctgctggcg gtcgccagtg
     1201 ccaccgtgcg cggcgtggtt gccggcctgc tcggcacggc gctggcgcag gcgctgctcg
     1261 ccggcatcgg cttcgcgatc gccggcaccg gcagcgccgt gttctacggc ttcgtgacct
     1321 tcttcctgtc cttcgtgccc ttcggtccgg tgttcgtgtg gggaccgctc gcctggcagc
     1381 tgtacggggc cggctcgacc ggcatggcga tctttctggc ggtctggggc gcggccgtgg
     1441 tcggcacgat cgacaacatc ctcaagccgc tgctgatcgg ccgcgggctg gagctgccgc
     1501 tgctgctggt gttcctcggc gtgctcggcg gcgcgctgtc cttcgggctg atcggcgtgt
     1561 tcgtcggtcc ggtgctgctc gcggtcggcc agaacctcgc gcggctgtgg ttcgtcgagg
     1621 gcgtcgcgcg ccggccggag ggctgatccc tgcggggagc aaggacggga gccggccaaa
     1681 aaaaacccgc cggatgcctc cgcaagcatt ccggcgggaa accaggggga gcgggacgcg
     1741 cgggcggatc ggccggtaag ggggagggaa ggccggcctg cgggtccgct cccggtacag
     1801 cctctcggga gcgagagtcg gtcacggcgt gcagcgcctg ccggcgctgc acgcggggtc
     1861 agtgatggat cgggagggac cggtcagccg agatcgaccg gcacgaagat gcgggcttcg
     1921 ccgcgctgga tcagcagcgc gatctgcttg ccggcatgcg cgaggcgctc cttcagctgc
     1981 gcgacggtgt tgaccggctc gccgttgatc gcgagcacca cgtcgccggg cgccacgccg
     2041 gcgcgcgcgg ccgggccgct cgcctgctgc accagcaggc cgctgtcgag gtcgttgccg
     2101 cgcttctcct gcggactcag cggccgcagc gccagcccga gccggccctg accgggcgcg
     2161 gcctcgtcgc cggcctcgcg cgcggcggcg cggaactcgc cgaccgtcgc cttcagcgtc
     2221 ttttcctcgc ccttgcggat caccgtcagc tcgacgtggc tgccgggctt gctgtcggcg
     2281 atgcgcggcg gcaggtcggc cgagcgttcg atggtctggc cgtcgagcgc gacgatcacg
     2341 tcgccggctt cgaggccggc ggcctcgccg gcgctgccgg gttcgacgct gctgaccagc
     2401 gcgccatgcg gcgtcttcag gccgaaggcg gcggcgagcg gcgccgagac atcctggatc
     2461 gcgacgccga gccggccgcg cgtgaccttg ccgtgcgcga cgatctgctg ctcgaccttc
     2521 atcgccacgt cgatcgggat cgcgaagctg atgccctgat agccgccggt gcgcgagtag
     2581 atctgcgagt tgacgccgat gacctcgccg tcggcattga acagcgggcc gccggagttg
     2641 ccggggttga ccgcgacatc ggtctgcagg aacggcacgt agttgtcatc gggcagcgtg
     2701 cgcgccttgg cgctgacgat gccggccgag acgctgttct cgaagccgaa cgggctgccg
     2761 atcgcgagca cccagtcgcc gacgcgcgag cgcgccgggt cgccgatgcg cacggtcggc
     2821 aggtcgctcg cgtcgatctt cagcacggcg atgtcgctct ggtcatcctt gccgagcacc
     2881 ctggccttga actcgcgccg gtcggtcagg cgcacggtga cgtgctcggc gccgtcgacg
     2941 acgtgggtgt tggtcaggat cacgccgtcg gcgcgcacga tgaagcccga gccttcgccg
     3001 cgcaccacgt ccggcgccgg cccgccgcgc ccgcgcaggc cgggcaggcc gaagcggcgc
     3061 aggaagtcat acagcggatc gttcgggtcg atctggatct gcggctgctg cgcggcggtg
     3121 cgcaccgtgc cttcgacctg gatgttgacg acggccgggc cgtagcgctc gaccagcgcg
     3181 ccgaagtcgg gcgtgacgat gcgcgccggc gccggcgtga ccgccggagc ctcggcatgc
     3241 gcctgctcga tcagctcgct gccgccgtgc agcacggcac cggcgccgaa ggccgcgacc
     3301 agcgccgcgg cgagcaggct gcggcgcagg ccggtggatt cgagcgccag tccgcgacga
     3361 tgaccggcac cgtgccgggc gcgcgcgaag cgggcgacgg gcgggacggt cgcggccggc
     3421 ggcgtctgcg ggttctgcga cgcttgcggg gactgcggaa ggttcgggtc ctgcgggttc
     3481 tgcgggttct gcgggttcat gtgcgtgcct cctcgcgggc gcttcggctg ccgcgttcaa
     3541 tgggcgcacg ataggcagga cgatgtcgcc acggcttacc cgtggcttaa cgcggcgtaa
     3601 cggccgtgcg tggaacctgc gcggaacctg tgtggaagga tgaggagggc agcgatggaa
     3661 attcatctgg tcggcggggc ggtgcgcgat gcgctgctcg ggcggtcggt cggcgagcgc
     3721 gactgggtgg tgaccggcgc cacgcccgat gaactgctgg cactcggcta caagcgcgtc
     3781 ggcaaggact tcccggtgtt cctgcacccc gaaaccggcg aggagtacgc gctggcgcgc
     3841 accgagcgca agaccgcgcc cggctaccac ggcttcgcga tcgacgccgc cccgcacgtg
     3901 acgctggagg acgacctcgc ccggcgcgac ctgaccgtca acgccatcgc ccaggccgcc
     3961 gacggccggc tgatcgatcc cttcggcggc cggcgcgatc tcgaagcgcg ggtgctgcgg
     4021 cacgtgtcgc cggccttcgc cgaggacccg gtgcgcatcc tcaggctcgc gcgcttcgcg
     4081 gcccgctatg caccgctcgg cttcacggtc gcgccggaaa cgcaggcgct gatgcgcgcc
     4141 atggttgccg ccggcgaggt cgatgcgctg cagcccgagc gcgtctggca ggagctggcg
     4201 cgcgcgctcg gcgaaccggt gccggggcgc ttcatcgagg tgctgcgcga ctgcggcgcg
     4261 ctcgcacggc tcctgcccga actcgaccgg ctctggggcg tgccgcagcc gccgcagcat
     4321 cacccggaga tcgacaccgg cgtgcacgtg ctgctggtgc tcgaacaggc cgtgcgcctc
     4381 ggcgccgacg tgccgacgcg ctacgcggcg ctgctgcacg acctcggcaa gggcacgacg
     4441 ccgccagccg agtggccgaa gcacactgcg cacgaggcac gcggcgcggc gctcgcccgc
     4501 gaggtcagcg agcgcctgcg cgcgccgaag gaattccgcg aactcgccgt gctggtcgcc
     4561 gaactgcata cgcgcgcgca ccgtgcgctg gagctgcgca cgacgacgct gctggaaacc
     4621 ctcgaacgcc tcgacgcgct gcgccgcccc gagcgcttcg cgcgctggct gctcgcctgc
     4681 gaggccgatg cgcgcgggcg caccgggctg gaaacgcggc cgtatccgca ggctgcatgg
     4741 ctcgcgcagg cgcgcgaggc ggcagcggcg gtgccggtgc agccgctggt cgagcgcggc
     4801 ctcaagggtc cggagttcgg cgaggcgctg cgtctggcac gcctcgatgc gctgcgcgcg
     4861 gcgcgcaagc gccgggccgc agcggcggac gcggcggggg aggggagtgc ggcgacatcg
     4921 tgagaagccg ttgcgacgac tggcatttcc attacatcag cgtaaggttg ctgcctgccg
     4981 tacccgtcaa ggagtcaccg ccatgaacgt cggcaacatc ggctcgtcct cgatcgagca
     5041 gttcatcagc aaccagcgtc cgccctccca gtccgaaggc gccccgcagc agcgccacgt
     5101 cgaagccgag cgccagcagg ccagcgaagc cctggtccgc tccgcccccg ccgccccgaa
     5161 ggacatcccc gccttcgacc cggaacggcg catcggcgcc tatatcgacg tcactgcctg
     5221 aaaccaccgc gcttacgccc ccacgccccg aagccatgcc ggttccgggg ccgggtggca
     5281 gtgccgctca cctttgctca aggcgcagca aacccgtcgc aagccgcagc atcccggccg
     5341 ccatcactgc cgatcccgct gccacgttcc acgtgaaacc ccgcccggcg ctccacgggc
     5401 gctgtctttg aactgccgca gggctttcac acaggccgcg tagcgtccgc ctgcccgcgg
     5461 accgcacgcg gcgcggtcgc tgccgggttc agggaacgca gagtccgtcc ggcagcttcc
     5521 ggtgccgtag ccgctgcgtc cgcaggcgtc gatccctcga ccgcttccct tccgtagcct
     5581 gcacggagcc tgttcccatg cgtacgcttg cctgttccgc cctggtgctc gtcaccggcc
     5641 tgatgcctgt tgccgtcgtt catgccgcgc cgttcccggc cggtccgctc ggcagctcgt
     5701 cccaatccac ctatttgctg ccgtcgatga ccggcgtcgc gctgaccccg ctgctgacgg
     5761 tcggcgatgc cgccggcaac ggttaccgga tggccggcat ccccgacggc ctcggggcct
     5821 tcgataacgg cgacggcagc ttcacgctgc tgatgaacca cgaactcggc gccagcgccg
     5881 gtgccgtgca tgcgcacggc gccaccggcg ccttcgtctc cgagtgggtc atcgacaaga
     5941 gcacgctgac ggtgctgagc ggtcgcgacc agatccagac cgtcaacctg ctgaacacct
     6001 ccaccggcca gtaccagcca gcgatcaacg ccacgctgac gcgcctctgc tcggccgacc
     6061 tgccggcgcc gaccgccttc ttcaacccgg gcagcggtct gggcaccagc acgcgcttct
     6121 tcatgaacgg cgaggagacc agcggcggcc gggcgttcgt gcacaccgtc accggcgccg
     6181 atgccggcca gagctacgag ttcgccgcgc tcggccacgc cgcctgggag aacgtcgtcg
     6241 cccggccgac tgccgatgac cgcaccgtcg tgctcggcac cgacgacacc catccgaacg
     6301 gcgatgccgc caacgccaag ggccgcgtct acgtctactt cggcgacaag caatcgaccg
     6361 gcaccgatct ggagaaggcc ggcctgacca acggcacgac ctgggcgatc aaggtcaacg
     6421 gcgcggcgat cgaggatcgt tcggctgcgg ccaacttcgg gctggacgcc gacggcaagg
     6481 ccagcttctc gctggtcgat gacggcagcg gcaccaactt cctgcgcccc gaggacatca
     6541 cctgggatac gcagaacccg gaccgcgcct acttcgtgac caccgacggt ctcgacgacg
     6601 ccgacccggc ctcgcgcacc agccggctgt ggcggatcac gctcaatgac gtgaacgacc
     6661 cggccgccgg tggcgtcatc gaactgctga tcgacggccg ccagcccggc cagccgcaga
     6721 tgatggacaa cctgacggtc gccgccgatg gcacggtgct gatccaggaa gacgtcggca
     6781 acaccgcgcg tctgggcaag gtctggcgct acgacccgaa cacgaagacc acggtcgagg
     6841 tcgccgccca cgacccggcg cgcttcctgc tcggcgcccc cggcttcctg acccaggacg
     6901 aggaatcctc cggcatcatc gacgtgaccg cgctgttcga cggcgtcgcc ggctacgaca
     6961 ccggcaccta ccagtacttc ctgctcgacg tgcaggcgca ctacgcgctc accgacccgg
     7021 cgctggtcga gggtgggcag ttgctgctga tggcggtgca ggcggtgccg gaaccgggca
     7081 gcctgtcgct gatggcggcg atgctcggct tcacgctcta cggcgcccgc cgcaagcgct
     7141 gacgcccgcc ccggcggcag agcgggccgc cgcggaatcc gtctgaacct gcctgaacct
     7201 gcctgaacgc acggcgcacc ggaccggcgg tgcgccgttt ttcattgtgc tggcacgggt
     7261 ggaagagggc cgcgcgcagc ggatagtcgc ccatgcggca ggcagtaccg gcttccacgc
     7321 cgcacatgcg cagccggagc agccccagta cgatgttccg gcgcgatgat ctcgccgctg
     7381 ccgtggaatc gtctcttgtc ctcgctgccc ctgtttcccg ataccgacgc gctcacggcc
     7441 cagccggtct gtgcgcccgc cgtcaccgcc gccgtggcca gactcgcggc cgaggacgat
     7501 gccgaagccc gcggtgcggt gttcacgcgc atcgaggtcg tcgagttcat tctggatctg
     7561 gtcgggtata cgccggaccg gccgctgcat gcctgccgtc tgctggaacc ggccgccggt
     7621 tgcggcgaat tcctgcgggt ggccgtgcgc cgcctgctcg cggcctggcg agcggctggt
     7681 agccccggcg acaccgacat cctggccgtg cttggtccgg cgctgcaggc cgttgagctg
     7741 catcacgaca gctgccgtcg tgcccgcgcc gcactcggcg aggaactggc cgcggccggc
     7801 atcggggcgg acacggcgca agccctgatc cggcaatggc tgcggcaggg cgacttcctg
     7861 ctgagcgcgc tgccggcctc gttcgacttc gtcgtcggca atccgcccta cgtgcggcag
     7921 gagctgattc cgccggtgct gctggccgaa taccgccgcc gttacgcgac gctctacgat
     7981 cgcgccgacc tgtacgtgcc gttcatcgaa cgcgcgctgt cgctgctcgc cgccggtggc
     8041 cggctcggct tcatctgccc ggaccgctgg atgaagaacc gctacggcgg cccgctgcgc
     8101 cggctggtgg ccgaaggctt ccacctgcag gcggtcatcg acatggatga aacgcccgcc
     8161 ttccacagcg aggtggccgc ttatccggcc atcgtcgtga ttgcccgcgc ccggcccggc
     8221 cgcacgcgca tcgcccatcg tccgccgctc gatgccggag tgctcgggcc gctggctgcc
     8281 gcgctgcagg ccgcggcaaa gcctgatccg tcgctgccgg aggtgcgcga gcaacaactg
     8341 gtctgcctgc gcggcagtga tgcaccgtgg ctgctggata ccgatgcgcg cacgaacctg
     8401 atccgccgca tcgaagccgg tttcccgctg ctcgaagcca ccggctgccg ggttggcatc
     8461 ggggtcgcga ccggagccga tcgcgtgttc atcggcgact acgccagcct cgatgtcgag
     8521 ccgcaacgca agctgccgct ggcgatgacg gccgacctcg aatccggtga gctgcgctgg
     8581 cgcgggctcg gggtcgtcaa tcccttcggc gacgatggcc ggctggtcga tctgcgcgac
     8641 tggccacggc tgcgcgcgta tctggagcgg caccgggacg tgatcgcagc ccgccactgc
     8701 gcgcgcaagt cccccggcaa ctggtaccgg accatcgacc gcatccaccc ggcgctggcc
     8761 gcggaaccga agctgctgat cccggacatc aagggctcgg cccacgtcgt ccatgaaccc
     8821 ggccgcctgt atccgcatca caatctgtat tacgtcacgg ccacggcctg ggacctgcac
     8881 gcgctgcagg ccgtgctgct gtcggggctg acccgggcct tcatcgccag ctactcgacg
     8941 cggatgcgtg gcggttatct gcggtttcag gcccagtacc tgcgccggct gcgactgccg
     9001 tactggcacg agctggccgg accgctgcgt caggaactga tccatgccgc ccgcagccgc
     9061 gacctcgccg cctgcgatcg ggccgtggcg cagctctatg ggctgagcgc agccgaacag
     9121 gcagaactgc tggcgcaacg ctgaatggca ctcgatctgg ccgattacga actgcgggcc
     9181 acagccgcgg tgcaggcgtt ctggaacagc cgcgagaccg cacgccagcg ccagcaggcg
     9241 gccggcctcc tcgatgcggg cgagcgcgcc ggcgtcaccg ccggcaggaa catggatggc
     9301 ttctgcgggc tgatcgtcgc gctggcacac gcgaacggac tcgacgcggc atgcatccat
     9361 cgcgcacggc gcctgctgac gctgcccggc tacttccggc cgaccaagca gtgggatctg
     9421 ctgatcgtcg atccgcacga tggcgccctg atcgccgcga tcgaactgaa aagtcaggtc
     9481 ggaccatcgt tcggcaacaa cttcaacaac cgtaccgaag aagccatcgg taccgcgcac
     9541 gacttctgga cggcataccg cgaaggcgca ctcggggcca cgctgcgccc gttcaccggc
     9601 tggctgatgc tggtcgagga cgcgcccggc tcgcgtcgtc cggtgcggga cgcatcgccg
     9661 catttccggg tgctgccgga atttgccgcc gcttcctacc tgcggcgcta cgagctgctg
     9721 tgcgagcgtc tggtgcgcga acagctctac accgccgctg cggtgatcgc ctcgccacgc
     9781 agcgccgccg gagatgggca gtggacggaa ctgtcaccgg ccaccgggct gcggaccttc
     9841 gtcagcgccc tggccggcca catggcggcg gttgccgcgc agcgcggctg aaaccgatca
     9901 ggcccgttcg gaccacgtat tcagccagca ggccggcggc atccgtctga acctgtcggg
     9961 acgaatggcc caccggagcg gcggtgcgcc gcttctgctt ctgcatgctc gctgccggtt
    10021 cggcggctgg cagaagcaag ccgtctgcac gcgcaccgcg cggctttcag cgcagcgtga
    10081 cctgattgcc gctgccggtg atctgcgtcg agacgttgcc gtggccatcg gtctttgc
//
LOCUS       DAOQIE010000128        10121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3354_length_10121_cov_7.997914, whole genome shotgun sequence.
ACCESSION   DAOQIE010000128 DAOQIE010000000
VERSION     DAOQIE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_10121_cov_7.997914"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1030)
                     /locus_tag="PK144_09420"
     CDS             complement(2..1030)
                     /locus_tag="PK144_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF65986.1"
                     /translation="MSRFAPRFLRPLLAVACAVVLTVGGSARADEPRDLLNVSYDVAR
                     EFYKDVNAQFVPYWQRSHGETVTVNQSHGGSSKQARAVIDGLAADVVTMNQQTDIDAI
                     AKTGLIRSDWRQALPFGSAPYGSTIVFLVRKGNPKGIQDWPDLGRDGVQIVIPNPKTA
                     GNGRYSYLGAWAWAKEQYGSDAAAREFVGKVFRNVPVLDAGGRAATTTFMQRGIGDVL
                     LTFESEVLQITREFSPDAFDVVIPSLSIDADAPVAVVDKVVDRKGSRALAEGYLRFLY
                     TPEGQRLAAKHYLRPRDPAVFAEFREQFRNVRLVTVDAAFGGWPQAQATHFADGGEFD
                     RLYQPGSR"
     gene            complement(1149..1739)
                     /locus_tag="PK144_09425"
     CDS             complement(1149..1739)
                     /locus_tag="PK144_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF65987.1"
                     /translation="MSLPQACTRFTFEDYLVWEPTQELRHEYLNGEVFAMTGARRNHV
                     TVTVNLTSALRNLVRGTPCRVYASDMKLRIASADAACYPDVSVSSDERDRRTDLFIAH
                     PLLIIEVLSPSTAAWDRSRKFDLYRQIDTLQEYVLIEPELRSVDVFRRRPAGDWLFQP
                     FRDDEAVELAALGCTLPMALIYEDVDFPPDAAADPD"
     gene            1897..3264
                     /gene="miaB"
                     /locus_tag="PK144_09430"
     CDS             1897..3264
                     /gene="miaB"
                     /locus_tag="PK144_09430"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNF65988.1"
                     /translation="MPRKLFIKTHGCQMNEYDSAKMGDVLRESHGLELTDDPAQADVL
                     LLNTCSIREKAQEKVYSELGRWRELKLERPGLVIGVGGCVASQEGAALRERAPFVDVV
                     FGPQTLHRLPALLAAADEHRGAVVDVSFPEIEKFDSLPEPRAEGPTAFVSVMEGCSKY
                     CTFCVVPYTRGEEVSRPFDDVLVEVATLAAQGVREVTLLGQNVNAYRGVMADGSIADF
                     AALIEVVAAIDGIDRIRYTTSHPIEFSDRLIEVYASVPELVDHLHLPVQSGSDRILAA
                     MKRRHTVLEYKSKIRRLRAIRPDLSLSTDIIVGFPGETEADFQATLKLIDEIGYDHSF
                     SFLYSPRPGTPAAELPDDVPLEQKKARLQLVIDRFNANAARIAAGMVGTVQRVLVERH
                     ARKDARQLAGRTPNNRVVNFDGPERLIGHFVDVRITEALPNSLRGEFVACEEIGMPAA
                     TVANA"
     gene            3285..4280
                     /locus_tag="PK144_09435"
     CDS             3285..4280
                     /locus_tag="PK144_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF65989.1"
                     /translation="MPAADGAVTQVLDLEPADNARLAQLCGHLDEHLRQIERRLGITI
                     ANRGGHFSLNGPEAATRIGAAVIEALYRAAGEEELNPARVHLFLQEAGADDIELPDPV
                     EAEPEEVAIRTRRGIVRGRGINQQRYLYAIQRHDINFGVGPAGTGKTYLAVACAVEAL
                     ERDEVRRLVLVRPAVEAGERLGFLPGDLVQKVDPYLRPLYDALYEMLGFERVAKLMER
                     SVIEIAPLAFMRGRTLNDAFIILDEAQNTTVEQMKMFLTRIGFGSTAVITGDTSQVDL
                     PKGVRSGLAHALEVLDGVDGISMTRFLPRDVVRHPLVQRIVIAYERHEQKQGGSA"
     gene            4277..4756
                     /gene="ybeY"
                     /locus_tag="PK144_09440"
     CDS             4277..4756
                     /gene="ybeY"
                     /locus_tag="PK144_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNF65990.1"
                     /translation="MSRLALDLQIETGAGALPPAADFRRWAEAALAGADFHDPCEMSV
                     RIVGIAESAALNAGYRHKDGPTNVLSFPFETPPGIVLDAELLGDLVICAPVVVREAGE
                     QHKPEAAHWAHLTVHGTLHLLGYDHLDDAEAEAMEALEIRILAGLGYPNPYADDERP"
     gene            4753..5631
                     /locus_tag="PK144_09445"
     CDS             4753..5631
                     /locus_tag="PK144_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNF65991.1"
                     /translation="MKEDRSGDSTGTTGRSWLERLGQALAGGPEDRDDLKDILKEAQA
                     RGIIDAEALSMMEGALAVAERQVRDVMIPRGQMIVMQADWPVERLLAEIIESGHSRLP
                     VIGEDRDEVLGILLVKDLLRWFASGAGEFDLRSFLRAPTFIPESKRLDVLLREFRANR
                     LHMAIVVDEYGGVAGLVTIEDLIEQIVGEIDDEHDEAGDDGDIRQIGEHEYIVRALTP
                     LADFNVRFDAHFDEDEADTVGGLVLLDLERVPKRGEVVEIERFRFRVLRADNRRVHLF
                     GLSVLDPVVPVDAANA"
     gene            5722..6372
                     /locus_tag="PK144_09450"
     CDS             5722..6372
                     /locus_tag="PK144_09450"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNF65992.1"
                     /translation="MDEWTQRRARMVEEQIEARGIHDPDVLAAMRRVPREDFVPEAMR
                     AWACDDSPLSIGDGQTISQPYIVALMIAAAAIRPGGRVLEVGTGSGYAAAVMAELAAH
                     VYTIERIEALAAQAAGRFARLGCTNIELRTGDGSGGWPEAAPFDAILVAASGPCVPEV
                     LRAQLAPGGRLVMPVDDGWLQRLVCVTREAAGSYREDFLCDVRFVPLIGAHGWEGA"
     gene            complement(6479..6913)
                     /locus_tag="PK144_09455"
     CDS             complement(6479..6913)
                     /locus_tag="PK144_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF65993.1"
                     /translation="MLPPGFYAGTFPDSAVGTVHRAPGDYPSGQEGDVLMVESTVIGI
                     PCPGLNGGPMFRHSEAFSFQVATDDQAETDRLWNAIVSNGGQESACGWYRDPWGLSWQ
                     ITPRALTAAIADPDRAAARRAFEAMMQMGKIDIATIEAARRG"
     gene            7004..8593
                     /gene="lnt"
                     /locus_tag="PK144_09460"
     CDS             7004..8593
                     /gene="lnt"
                     /locus_tag="PK144_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNF65994.1"
                     /translation="MTAPLADPAASPSAVSSGSTDSGAPAAGRLAAGHPLALLLAPIA
                     GLLLPLAFAPYRLWPLGLLLPAVLLWLIDGERPRRALLIGLLFGLGSYGHGIWWIYIS
                     LHRYGGAPPAFAGLATFLVVLLMAAYVALFAGLLNRLAPAPGARRWLLAAPGLWALLE
                     WVRSWAFTGFPWLALGYAQVESPLAGFAPILGTFGLGALVMLVAGSLRLALARLRSGA
                     RAALAPLAVALLVPALGFGLGRIDWSAPAGRVLAVGLAQGNIAQEMKWRPGKLEETIA
                     VYAGLSQRAQAQGHRDLIVWPETALPAFLSDLDDNFLAELRAWAAETGTDLLIGAPDG
                     DRARRDYRNAVASIGRTPGVYAKHRLLPFGEYLPLRGLLMIFRDFVQIPMADFTAGAA
                     DQPLLVAAGVPVGVSICFESVFGSEVRRALPAAQLLVVVSNDAWFGDSLAPHQHLQIA
                     QMRALEAARPLVRATNTGLTAIVDERGRITAQAPAFVETVLDGDVQPRSGVTPYVRFG
                     DGPAAGLAAVLLLAGALRRRG"
     gene            complement(8648..8821)
                     /locus_tag="PK144_09465"
     CDS             complement(8648..8821)
                     /locus_tag="PK144_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF65995.1"
                     /translation="MQRLIHRQRLRRIQDQQQQHALRRGRGQVWELAQMLDNADGKRD
                     SDRPAAEPVRQPH"
     gene            complement(8917..9432)
                     /locus_tag="PK144_09470"
     CDS             complement(8917..9432)
                     /locus_tag="PK144_09470"
                     /inference="COORDINATES: protein motif:HMM:NF018946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HNF65996.1"
                     /translation="MTEPRHRLAEAYARLLERLHLHDADRDASEATPPLRARIDRAAA
                     DAHHLGELTKEEAGLLAHWARRDLADAGRHLAETGQELGSWLRFDTELVEERLLDWFG
                     RAADQSRLELLEFADTLERLSHYETGEVTGPGTLACEACGERIEFRGTGTIPSCPKCG
                     HTRFERPVDPS"
     gene            9589..>10121
                     /locus_tag="PK144_09475"
     CDS             9589..>10121
                     /locus_tag="PK144_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF65997.1"
                     /translation="MEESYQPQAVEAAAQRYWNEQRSFEVAADPAREKFYCLSMFPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGRNVVQPMGWDAFGMPAENAAIKNSVAPARWTY
                     ENIDYMRAQLKSLGLGIDWSRELATCTPDYYRWEQWLFTRLFEKGVIYRRNGVVNWDP
                     VDQTVLANEQVIDGRGWR"
BASE COUNT         1463 a         3497 c         3572 g         1589 t
ORIGIN      
        1 gtcagcggct gccgggctgg tagagacggt cgaactcgcc gccatcggcg aagtgggtcg
       61 cctgcgcctg cggccagccg ccgaaggcgg catcgacggt cacgaggcgc acgttgcgga
      121 actgctccct gaattcggcg aacaccgccg gatcgcgcgg gcgcagatag tgcttcgcgg
      181 ccaggcgctg gccctcgggc gtgtacagga agcgcaggta gccctcggcg agggcgcggc
      241 tgcccttgcg atcgacgacc ttgtcgacga cggcgaccgg cgcatcggca tcgatgctga
      301 gcgacgggat cacgacatcg aaggcgtcgg ggctgaattc cctggtgatc tgcagcactt
      361 cgctctcgaa ggtcagcagc acgtcaccga tgccgcgctg catgaaggtc gtcgtcgcgg
      421 cacggccgcc ggcgtcgagc accggcacgt tgcggaacac cttgccgacg aactcgcgcg
      481 cggcggcgtc gctgccgtat tgctccttcg cccaggccca ggcgccgagg tagctgtagc
      541 ggccgttgcc ggccgtcttc gggttcggga tgacgatctg cacgccgtcg cgcccgagat
      601 cgggccagtc ctgaataccc ttcgggttgc ccttgcgcac caggaagacg atggtcgagc
      661 cgtacggcgc cgagccgaac ggcagcgcct gacgccagtc gctgcggatc aggccggtct
      721 tcgcgatcgc gtcgatgtcg gtctgctggt tcatcgtcac gacatcggcg gcgagcccgt
      781 cgatgacggc gcgcgcctgc ttgctggagc cgccgtgcga ctggttgacg gtgacggtct
      841 cgccgtggct gcgctgccag tacggcacga actgcgcgtt cacgtccttg tagaactcgc
      901 gcgccacgtc gtacgagacg ttcagcaggt ccctgggctc atcggcgcgg gcactgccgc
      961 cgacggtgag cacgactgcg caggcgacag cgagcagcgg gcggagaaag cggggggcga
     1021 agcgggacat gcggcaatac ctcggcagga gcgcatctca tggcggccga gcatatccgc
     1081 ggattttata ttgcttaaaa taataatgaa ttagttttat attctttttt tgtataaatg
     1141 cgcgtgcatc agtccggatc ggcagcagcg tcgggcggaa aatccacatc ctcgtagatc
     1201 agcgccatcg gcagcgtgca gccgagcgcg gccagttcga ccgcctcgtc gtcgcgaaac
     1261 ggctggaaca gccagtcgcc ggcgggcctg cgccggaaca cgtcgacgct gcgcagctcc
     1321 ggctcgatca gcacgtattc ctgcagtgtg tcgatctgcc ggtacagatc gaacttgcgg
     1381 ctgcggtccc aggcggccgt cgagggcgac agcacctcga tgatcagcag cggatgggcg
     1441 atgaacagat cggtgcgccg gtcgcgttcg tcactgctca cgctcacgtc cgggtagcag
     1501 gccgcatcgg cgctggcaat gcgcagtttc atgtcgctgg catagacgcg gcagggcgtc
     1561 ccgcgcacga gattgcgcaa ggccgacgtc aggttgacgg tcaccgtgac gtggttgcga
     1621 cgggcgccgg tcatcgcgaa gacttcgccg ttcaggtact cgtggcgcag ctcctgcgtc
     1681 ggctcccaga ccagatagtc ctcgaacgtg aaacgggtgc aggcttgcgg caggctcatg
     1741 tccgtgactc cgggcaggcg acgggtccga gtatggcccg gctcgccggg ccgtcgcgag
     1801 tgtttgtcgg cgccggcgcc ggtgctagac tcccgccccc gcatcccctt gagtgcgttg
     1861 aaaactttac tgacgctgct ccgtcgcagg aacccgatgc cgcgcaaact ctttatcaag
     1921 actcacggtt gccagatgaa cgagtacgac tccgcgaaga tgggagatgt actgcgcgag
     1981 tcacacggtc tggaactcac cgacgatccc gcccaggccg atgtcctgct gctgaacacc
     2041 tgttcgatcc gcgagaaggc gcaggagaag gtctactccg agcttggccg ctggcgcgag
     2101 ctgaagctcg aacgcccggg cctcgtcatc ggcgtcggcg gctgcgtcgc cagccaggaa
     2161 ggcgcggcgc tgcgcgagcg cgcgcccttc gtcgacgtgg tgttcggccc gcagacgctg
     2221 caccggctgc cggcgctgct cgcggcggcc gacgagcacc gcggcgccgt cgtcgacgtg
     2281 tccttccccg agatcgagaa gttcgacagc ctgcccgagc cgcgcgccga gggaccgacc
     2341 gcgttcgtgt cggtcatgga aggctgctcg aagtactgca ccttctgcgt cgtgccgtac
     2401 acgcgcggcg aggaggtctc gcggccgttc gatgacgtgc tggtcgaggt cgcgacgctg
     2461 gcggcgcagg gcgtgcgcga ggtcacgctg ctcggccaga acgtcaacgc ctaccgcggc
     2521 gtcatggccg atggctcgat cgccgatttc gcggcgctga tcgaggtggt cgcggcgatc
     2581 gacggcatcg accgcatccg ctacacgacc tcgcatccga tcgagttcag cgaccggctg
     2641 atcgaggtct atgccagcgt gccggaactg gtcgatcacc tgcacctgcc ggtgcagagc
     2701 ggctcggacc gcatcctcgc ggcgatgaag cgccggcaca cggtgctcga atacaagtcg
     2761 aagatccgca gactgcgcgc gatccggccg gatctgtcgc tgtcgaccga catcatcgtc
     2821 ggcttccccg gcgagaccga ggccgatttc caggcgacgc tgaagctgat cgacgagatc
     2881 ggctacgacc acagcttcag cttcctgtac agcccgcgcc cgggcacgcc ggccgccgaa
     2941 ctgcccgacg acgtgccgct ggagcagaag aaggcgcggc tgcagctcgt catcgaccgc
     3001 ttcaacgcca atgccgcgcg catcgccgcc ggcatggtcg gcacggtgca gcgcgtcctg
     3061 gtcgagcgcc acgcgcgcaa ggacgcccgc cagctcgccg ggcgcacgcc gaacaaccgc
     3121 gtcgtcaact tcgacgggcc cgagcgcctg atcgggcatt tcgtcgacgt gcgcatcacc
     3181 gaggccctgc cgaactcgct gcgcggcgag ttcgtcgcct gcgaggagat cggcatgccg
     3241 gctgcgacgg tggcgaacgc gtgaataccg tcctgcccgc accgctgccc gccgccgacg
     3301 gtgccgtcac gcaagtcctc gacctcgaac ccgccgacaa cgcccgtctc gcccagctct
     3361 gcggccacct cgacgagcac ctgcgccaga tcgagcgccg gctcggcatc accatcgcca
     3421 accgcggcgg gcatttcagc ctcaacggtc ccgaggccgc gacgcggatc ggtgccgcgg
     3481 tcatcgaggc gctgtaccgc gccgccggcg aggaggagct gaacccggcc cgggtgcacc
     3541 tgttcctgca ggaggccggc gccgacgaca tcgagctgcc cgatccggtc gaggccgagc
     3601 ccgaggaggt cgcgatccgc acccggcgcg gcatcgtgcg cgggcgcggc atcaaccagc
     3661 agcgctacct gtacgcgatc cagcgtcacg acatcaattt cggcgtcggc ccggccggca
     3721 ccggcaagac ctatctcgcc gtcgcctgcg cggtcgaggc gctggagcgc gacgaggtgc
     3781 gccggctggt gctggtgcgt ccggcggtcg aggccggcga gcggctcggc ttcctgcccg
     3841 gcgacctggt gcagaaggtc gatccgtatc tcaggccgct gtacgacgcg ctctacgaga
     3901 tgctcggctt cgagcgcgtc gccaagctga tggagcgcag cgtcatcgag atcgcgccgc
     3961 tcgcgttcat gcgcgggcgc acgctcaacg acgccttcat catcctcgac gaggcgcaga
     4021 acaccacggt cgaacagatg aagatgttcc tgacccgcat cggcttcggc tcgacggcgg
     4081 tgatcaccgg cgacacctcg caggtcgacc tgccgaaggg cgtgcgctcg ggtctcgcgc
     4141 atgcgctgga ggtgctcgat ggtgtcgacg gcatcagcat gacgcgcttc ctgccgcgcg
     4201 acgtggtccg ccacccgctg gtgcagcgca tcgtcatcgc ctacgagcgc cacgagcaga
     4261 agcagggcgg cagcgcatga gccggctcgc gctcgacctg cagatcgaga ccggcgccgg
     4321 cgcgctgccc ccggcggccg atttccgccg ctgggccgaa gccgcgctgg ccggggcgga
     4381 ttttcacgac ccctgcgaga tgagcgtgcg catcgtcggc atcgccgaga gcgcggcgct
     4441 caacgccggc taccggcaca aggacggccc gaccaacgtg ctgtccttcc cgttcgaaac
     4501 cccgcccggc atcgtgctcg atgccgagct gctcggcgat ctggtcatct gtgccccggt
     4561 cgtcgtgcgc gaggccggcg aacagcacaa gcccgaagcc gcgcactggg ctcacctgac
     4621 cgtgcacggc accctgcacc tgctcggcta cgatcacctc gacgatgccg aagccgaggc
     4681 catggaagcg ctcgaaatcc gcattctggc cggtctcggc tatccgaacc cctacgcaga
     4741 cgacgaacga ccatgaagga agaccgatcc ggcgacagta ccggcacgac cggccgcagc
     4801 tggctggaac gcctcggaca ggccctcgcg ggcggacccg aggatcgaga tgacctgaag
     4861 gacatcctca aggaagcgca ggcgcgcggc atcatcgatg ccgaggccct gtcgatgatg
     4921 gaaggcgcgc tggcggttgc cgagcggcag gtgcgcgacg tcatgattcc gcgcgggcag
     4981 atgatcgtca tgcaggccga ctggccggtc gagcgcctgc tcgccgagat catcgagtcc
     5041 ggccactcgc gcctgccggt gatcggcgag gatcgcgacg aggtgctcgg catcctgctg
     5101 gtcaaggacc tgctgcgctg gttcgcgagc ggtgccggcg agttcgacct gcgcagcttc
     5161 ctgcgcgccc cgaccttcat ccccgagagc aagcgcctcg acgtgctgct gcgcgagttc
     5221 cgcgccaacc gcctgcacat ggcgatcgtc gtcgacgagt acggcggggt cgccggcctc
     5281 gtgaccatcg aggacctgat cgagcagatc gtcggcgaga tcgacgacga gcacgacgag
     5341 gccggcgatg acggcgacat ccgccagatc ggcgagcacg agtacatcgt gcgcgcgctg
     5401 acgccgctcg cggacttcaa cgtccgcttc gacgcgcatt tcgacgagga cgaggccgac
     5461 accgtcggcg gcctggtgct gctcgacctc gaacgcgtgc ccaagcgcgg cgaggtcgtc
     5521 gagatcgagc gcttccgctt ccgcgtgctg cgcgccgaca accgccgcgt gcacctgttc
     5581 gggctgagcg tgctcgaccc ggtggtgccg gtggacgcag cgaacgcctg aggccgggca
     5641 gcggcgcgtg cgcagcgccg ccatgcgctc cgtatgccgt cgcgcccggc gtcagccggg
     5701 ctggtgtcag gaggaaccgt cgtggacgag tggacgcagc ggcgcgcgcg catggtcgag
     5761 gagcagatcg aggcgcgcgg catccacgat ccggacgtgc tcgcggcgat gcgccgcgtg
     5821 ccgcgcgagg acttcgtacc cgaggcgatg cgcgcatggg cctgcgacga ttcgccgctg
     5881 tcgatcggcg acgggcagac gatctcgcag ccgtacatcg tggcgctgat gatcgccgca
     5941 gcggcgatcc ggcccggcgg gcgcgtgctc gaagtcggca ccggctcggg ctacgcggcg
     6001 gcggtcatgg ccgagctggc cgcgcatgtc tacaccatcg agcgcatcga agcgctggcc
     6061 gcgcaggcgg ccgggcgctt tgcgcgcctc ggctgcacga acatcgaact gcgcaccggc
     6121 gacggcagcg gcggctggcc cgaggcggcg cccttcgacg cgatcctcgt cgccgcgagc
     6181 ggtccgtgcg tgcccgaggt gctgcgggcg caactggcgc cgggcgggcg gctcgtgatg
     6241 ccggtcgatg acggctggct gcagcggctc gtgtgcgtga cgcgcgaggc agccggttcg
     6301 taccgggaag acttcctgtg cgacgtgcgc ttcgtgccgc tgatcggcgc gcacggctgg
     6361 gaaggggcgt gagccggcgc aggccggagc gacgaaggca ttccggccct gctgcatgga
     6421 ttccccggcg aaattgccgg ggttcgcaag cgcacgcgaa cctgcgggct gccgggcgtc
     6481 agccgcgccg ggccgcctcg atcgtcgcga tgtcgatttt gcccatctgc atcatcgcct
     6541 cgaaagcgcg cctggcggcg gcgcgatcgg gatcggcgat ggcggccgtc agcgcgcgcg
     6601 gcgtgatctg ccacgacaga ccccatgggt ccctgtacca gccgcaggcg ctttcctgac
     6661 caccattgct gacgatcgcg ttccacagcc gatcggtttc ggcctggtcg tcggttgcga
     6721 cctggaacga gaaggcctcg ctgtgcctga acatcgggcc cccattcagg ccggggcaag
     6781 ggatgccgat caccgtggac tcgaccatca gcacatcgcc ctcctggccc gatggatagt
     6841 cgccgggcgc gcgatgcacc gtgcccactg cgctgtccgg gaaggtgccg gcgtagaacc
     6901 ccgggggcag cagggcgcgg gctgacggcg cctcgcgctc cgtcgcggtc cgcccgggcg
     6961 catcggctag actgccgctc ccgccgctgc caccccgttc gtcgtgaccg caccgctcgc
     7021 cgatcctgcc gcttcgcctt ccgccgtttc ctccggttcc acggattccg gtgccccggc
     7081 cgccggtcgc cttgctgccg gccatccgct cgcgctgctg ctggctccga tcgcgggtct
     7141 gctgctgccg ctggcgtttg cgccgtaccg gctctggccg ctcgggctgc tgctgccggc
     7201 cgtgctgctg tggctgatcg acggcgagcg cccgcggcgg gcgctgctga tcggcctgct
     7261 gttcgggctc ggcagctacg gccacggcat ctggtggatc tacatcagcc tgcaccgcta
     7321 cggcggtgcg ccgccggcgt tcgcggggct tgcgaccttt ctggtcgtgc tgctgatggc
     7381 ggcctacgtc gcgctgttcg ccgggctgct gaaccggctc gcgcccgcgc ccggcgcccg
     7441 gcgctggctg ctcgcggcac cggggctgtg ggcgctgctc gaatgggtgc gttcgtgggc
     7501 cttcaccggc tttccgtggc ttgcgctcgg ctatgcgcag gtcgagtcac cgctggccgg
     7561 ctttgcgccg atcctcggca ccttcgggct cggtgcgctc gtgatgctgg tggccggctc
     7621 gctcaggctc gcactcgcgc ggctgcgcag cggtgcgcgc gccgcgcttg cgccgctcgc
     7681 cgtcgcgctg ctcgtgccgg cactcggctt cgggctcggg cgcatcgact ggagcgcgcc
     7741 ggcgggtcgg gtgctggcgg tcggcctcgc acagggcaac atcgcgcagg agatgaaatg
     7801 gcggcccggc aagctggagg agacgatcgc cgtctatgcc ggcctgtcgc agcgcgcgca
     7861 ggcgcagggg catcgtgacc tgatcgtgtg gccggagacg gcgctgccgg ccttcctgtc
     7921 cgatctcgac gacaactttc tcgcagaact gcgcgcctgg gcggccgaaa ccggcaccga
     7981 cctcctgatc ggcgcgccgg acggtgatcg cgcccggcgc gactaccgca acgctgtcgc
     8041 gtcgatcggg cgcacgcccg gcgtctacgc caagcaccgg ctgctgccct tcggcgaata
     8101 cctgccgctg cgcgggctgc tgatgatctt ccgcgacttc gtgcagatcc cgatggccga
     8161 cttcacggcc ggagccgccg accagccgct gctggtcgcg gccggcgtgc cggtcggcgt
     8221 gtcgatctgc ttcgagtcgg tgttcggcag cgaagtgcgc cgcgcgctgc cggcggcgca
     8281 gctgctggtg gtggtcagca acgacgcctg gttcggcgac agcctcgcgc cgcaccagca
     8341 cctgcagatc gcgcagatgc gggcgctgga ggccgcgcgg ccgctggtgc gcgcgaccaa
     8401 caccggcctc acggcgatcg tcgatgaacg cgggcggatc acggcgcagg cgccggcctt
     8461 cgtcgagacc gtgctcgatg gcgacgtgca gccgcgcagc ggcgtcacgc cctacgtgcg
     8521 cttcggggac gggccggcgg cagggctggc ggccgtgctg ctgctggccg gagccctgcg
     8581 ccggcggggc tgacgagacc ggcggttgcg gccggcttcc ctgccggacc gccggttcgt
     8641 catggcgtca gtgcggttgc cggaccggct cggcggcggg gcgatcggaa tcgcgcttgc
     8701 cgtcggcgtt gtcgagcatc tgggcgagtt cccagacctg gccgcggcca cgacgcagcg
     8761 cgtgctgctg ctgctggtcc tggatacggc gcaggcgctg ccggtggatg agtcgctgca
     8821 tggcggtcgt accggtaacg ggccccgagg cgggcgagcg cgagcatacg cgcgtgttcc
     8881 tgaaaatgaa atttttttaa gccgggaact tccgtcttac gacggatcga cggggcgttc
     8941 gaagcgcgta tgcccgcact tcgggcacga cggaatcgtg ccggtgccgc ggaactcgat
     9001 gcgctcgccg caggcctcgc aggccagcgt gccggggccg gtgacctcgc cggtctcgta
     9061 atgactgagc cgttccagcg tgtccgcgaa ttccagcagc tccagccgtg actggtcggc
     9121 cgcgcgcccg aaccagtcga gcaggcgctc ctcgaccagt tcggtgtcga agcgcagcca
     9181 gctgccgagc tcctggccgg tctcggcgag atggcggccg gcgtcggcga ggtcgcgccg
     9241 cgcccagtgc gcgagcaggc cggcttcctc cttcgtcagt tcgccgaggt gatgggcgtc
     9301 cgcggcggcg cggtcgatgc gcgcgcgcag cggcggcgtg gcttccgagg cgtcccggtc
     9361 ggcgtcgtgc aggtgcaggc gttcgagcag ccgggcgtag gcctcggcga ggcggtggcg
     9421 cggttcggtc atggcggggt ttcctccgta cggcgttccc ggcctgcagt ctcgacccgg
     9481 cgctcacggc cggcaagcac ggtccgtgct gcactgtgcc gtacggcgcg gcctcgggta
     9541 tgcttcgtcc ctttttcgga acgccacctg agccccgggc cgagcatgat cgaagagtcc
     9601 taccagccgc aggccgtcga ggccgccgcg cagcgctact ggaacgagca gcgcagcttc
     9661 gaggtcgccg ccgacccggc gcgcgagaag ttctactgcc tgtcgatgtt cccgtacccg
     9721 agcggccggc tgcacatggg ccacgtgcgc aactacacga tcggcgacgt gatcgcgcgc
     9781 taccagcgca tgctcggcag gaacgtcgtg cagccgatgg gctgggacgc cttcggcatg
     9841 ccggccgaga acgccgcgat caagaacagc gtcgcgccgg cgcgctggac ctacgagaac
     9901 atcgactaca tgcgcgcgca gctgaagtcg ctcggtctcg gcatcgactg gtcgcgcgaa
     9961 ctcgccacct gcacgcccga ttactaccgc tgggagcagt ggctgttcac gcgcctgttc
    10021 gagaagggcg tcatctaccg caggaacggc gtcgtcaact gggatccggt cgaccagacc
    10081 gtgctcgcca acgagcaggt catcgacggc cgcggctggc g
//
LOCUS       DAOQIE010000129         9956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3446_length_9956_cov_8.442582, whole genome shotgun sequence.
ACCESSION   DAOQIE010000129 DAOQIE010000000
VERSION     DAOQIE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_9956_cov_8.442582"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1350
                     /gene="moaA"
                     /locus_tag="PK144_09480"
     CDS             334..1350
                     /gene="moaA"
                     /locus_tag="PK144_09480"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNF65998.1"
                     /translation="MSADSLQTPAPKLIDRFGRHIDYIRLSVTDRCDFRCVYCMAEDM
                     NFLPRAKVLTLEEMAAVGRAFAELGVRKIRLTGGEPLVRRDVMTLVRELGALAQLREL
                     VITTNGSQLERYAAELKAAGVRRLNISLDSLRPERFRRITRTGDLEVVLRGIRAAQAA
                     GFERTKLNAVVLKHRNHDEVVDLVRFACDEGLDLSFIEEMPLGVIGDHDRAEAYYSSD
                     DIRRDLAGHFDLIPTTEQTGGPSKYYRVGGSASRIGFISPHSHNFCGDCNRVRVSAEG
                     QLLLCLGQEHSVDLRHVLRAHPGDPERLKQAIVEAMHIKPKGHDFNLKAQPVILRHMN
                     MTGG"
     gene            1450..1917
                     /locus_tag="PK144_09485"
     CDS             1450..1917
                     /locus_tag="PK144_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF65999.1"
                     /translation="MQRSLYIDPQKCTGCLQCEMACSYENEQAFNPSKSRIKVFAFHE
                     EGRFVPYTCTQCAEAWCLHACPVEAITKNPATGAMEVNEKTCVGCKVCTIACPFGTVN
                     YNTATGKVIKCDLCQGDPACAKACPTGAITYVDSSWTGLDKMRAWAAKANTAA"
     gene            1937..3793
                     /locus_tag="PK144_09490"
     CDS             1937..3793
                     /locus_tag="PK144_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNF66000.1"
                     /translation="MGWNNKVLRVNLSTGSMSVEPLRMDWARDYLGQRGLATKYLVEE
                     TDPKVDPLSPDNKMIMTTGPLTGTEASTSGRYSVVTKGPLTNAIACSNSGGFFGAELK
                     YAGWDMIIFEGRSPKPVYLSIVDDKAELLDAAEIWGKSVWETEHWVHKKHHDPLVRVA
                     TIGAGAEKGVLYAAIVNDLHRAAGRSGVGAVMGSKNLKAVVVRGTIGMNKVKDPVAFH
                     AASEAAKKVLADNAVTGAGLPKYGTQVLMNVINEIGAMPTRNWREVQFEGASAISGEA
                     MYEPRQSDGKPNLVRNGACFGCTIACGRISTIDPTHFSVQGEGKERYKHASGGLEYEA
                     AWALGSDTGIDDLDALTYANFLCNEHGLDPISFGATVAAAMELYEVGALTKEQTGGIE
                     FKFGNAEALCKAAELTGKGEGFGLELGLGSRRLCEKYGHPEFSMTVKSQEFPAYDARG
                     IQGMGLTYATSNRGACHLRSYTVASEVLGIPVKTDPLVTDGKPELVKAFQDATAVVDS
                     TGLCVFTTFAWTLADFQPQIQAACEGDWSMDKLNEVGERIWNLERQYNLDAGLTAADD
                     TLPKRLLTEAAKTGPAAGRVNDLGKMMPVYYELRGWTADGKLKPETRKRLGV"
     gene            3867..5180
                     /locus_tag="PK144_09495"
     CDS             3867..5180
                     /locus_tag="PK144_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF66001.1"
                     /translation="MIHRYVIVGAGPAGVIAAETLRTTDPDADITLIGDEPERPYSRM
                     AIPYLLIEKIGEEGTHLRKGNDHYDSKGIRVLRDRVEKVDAKTKRLTLKSGGAMDYDK
                     LLVCTGSRAVRPPVPGFDLPGVHTCWTLEDARNIARLAEKGAKVVLVGAGFVGCIVLE
                     ALALRGVDLTVLEMGDRMVPRMMSPIAGGLIKSWCEKKGVRVITSTRVTGITENGGLM
                     NKLVNTLTGKPTRALKVTLNTGETLDADLVVSAAGVAPNIEFLQGCGLEIERGILIDH
                     YMQTNVPDIYAAGDVAQGRDFSTGGLEVHAIQPTASEHGRTAGLNMAGVPTYYRGSFN
                     MNVLDTLGLISSSFGLWMGVEGGDRVEMCEPDRFRYLQLQFDGDIMVGATSLGLTQHV
                     GVLRGLIQGKVPLGPWKAKLMDDPTRIMEAYLACNFAARVAPKAA"
     gene            5189..5440
                     /locus_tag="PK144_09500"
     CDS             5189..5440
                     /locus_tag="PK144_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNF66002.1"
                     /translation="MQITLKLYATLTPHLPAGARRNVATLEVADGASVAGVIDDLHLP
                     PALVHLVLVNGSFVGPEDRAARVLRAGDALAVWPPVAGG"
     gene            5433..5738
                     /locus_tag="PK144_09505"
     CDS             5433..5738
                     /locus_tag="PK144_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66003.1"
                     /translation="MAESSALLWQGEMALDRREFLRVLPAACAGRPHAQAGDAIRVEL
                     GAGARLRIELGPTRERRIALLALPATVITILADGPVPAALASAFLRDFENAYRRGGG"
     gene            complement(5743..6135)
                     /locus_tag="PK144_09510"
     CDS             complement(5743..6135)
                     /locus_tag="PK144_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66004.1"
                     /translation="MNLLPARLRWIAVLPALLPALLPSLPALAGEPLEVYVARLSAAD
                     HYNSSGERLRSAAAIIRQDRANLHRYGVRDPEDETDRFFAAADNRARLERLLADGFLD
                     RTTERIIVEDTPLVRVEVWPNHVEVRLK"
     gene            complement(6192..6662)
                     /locus_tag="PK144_09515"
     CDS             complement(6192..6662)
                     /locus_tag="PK144_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNF66005.1"
                     /translation="MTPARPRRLLIVFHSQSGRTARLAAAVAAGARECGGIETLLRRA
                     PEAGLDDLLGCDGLLLGTPENFGYMSGLLKDFFDRTYYPAEGRTVGLPYAVFVSCGND
                     GSGAVREIGRIARGYGWKEVAEALIVRGEPDDEALQRAHELGAALAAGLELGIF"
     gene            7122..7490
                     /locus_tag="PK144_09520"
     CDS             7122..7490
                     /locus_tag="PK144_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66006.1"
                     /translation="MSKTILIIDDSPSLRQIVAYTLTQAGHAVVEAASGDEALQRLDG
                     RVLNLVICDLHMPGINGLEFVAKLKARHEYRFVPVLMLTTEASDTCREAGRAAGAKAW
                     MVKPFQPAALCAAVTRLLAA"
     gene            7499..7813
                     /locus_tag="PK144_09525"
     CDS             7499..7813
                     /locus_tag="PK144_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005905535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF66007.1"
                     /translation="MLHIDGNGRRLTLAGRFTIYHVAELLAYLRGLELAPMLEIDLSG
                     VTDFDSAGVQLLLALQREQQQHGRALRLLQHSAAVLDVFTLYELSAHFGDPVLLPAGG
                     GA"
     gene            7810..>9956
                     /locus_tag="PK144_09530"
     CDS             7810..>9956
                     /locus_tag="PK144_09530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNF66008.1"
                     /translation="MNLPLQAVMQTFCIEARERIAELEAGLLTLEQAPSDTEALSAVF
                     RAAHTIKGSAGMLGLDAVSAFTHGFEDVLDALRTGARRFEHRLGELLLLAADHLALLL
                     DAAEAGEVPDAALQAAGTALLQALHEEPQASAVPALPPAPAPMPVERERPAAETGGPE
                     FWHLSVRFGPDALRNGFDPLGCLRYLHTLGRVLRVVTLTDALPDWAGFDAETCFLGFE
                     IDLDSSHERETIAATFEFVADDCTLHILPPNSQAAQYLDLIRALPEDDLSIGEILVRS
                     GCLSRLELEQGLRAQAQAAAGSKRLGELLVDRHCVDADVVRAALERQAHGRTRRTAEA
                     HTLRVQAPRLDELIERIGELVTVGAGATLLARELGLPALDELATTLNRLVSGIRDNAL
                     QLRMVPIGETFARFPRVVRDLGQELGKDIDLELCGEDTELDKAIVEPLVDPLMHLVRN
                     AVDHGIEPLAVRRARGKPGVGRVRLSARHEGANMLIEVADDGGGLDGERIRSKAVAAG
                     LIGAGEHLDAQGLARLIFAPGLSTRDTVTDVSGRGVGLEVVQRNIEALRGSIEIESEA
                     GAGTTVRLRLPLTLAIIDGFLIRCAGTSFVLPLDQVEECLDFDVAALHAPPRQLDLRG
                     RPLPLIRLHEFFGLGTPCGRRASVVVVRCGERRAGLLVDELLGEFQTVIKPLGPLFEH
                     LRALGGTTILGSGEVALIVDVPALFALAAARTPP"
BASE COUNT         1603 a         3436 c         3402 g         1515 t
ORIGIN      
        1 ccggcggccc ggcctgcggc cgtgacggtg cggccgcagg ggcagccggc gggctggcct
       61 ctgcggatgc cgggcgcggc atccgcggcc tgccggccgg cgttgctagg ctggtacgtg
      121 tgccgtgcgt accggaggcg gtgggacagt ctgtaccacc aaaaagccgc tgccggggca
      181 ggttgccgac gcgcaagctg gcaatatcag gtgcttgcaa aactgtgctc aaacgccctg
      241 gcctctggcc cctgccttgc atgtacgccg atccagaacc tgcgcaccgg accgtcagga
      301 ccgggtgacg cccagaagag gatgccttcc gaagtgtcag cggactccct ccagacgccg
      361 gctccgaaac tgatcgaccg tttcggccgg cacatcgatt acatccgtct gtcggtcacc
      421 gaccgctgcg atttccgctg cgtgtactgc atggccgagg acatgaactt cctgccgcgc
      481 gcgaaggtgc tgacgctgga ggagatggcc gcggtcggcc gggctttcgc cgaactcggc
      541 gtgcgcaaga tccgcctcac cggcggcgag ccgctggtgc ggcgcgacgt gatgacgctg
      601 gtgcgcgagc tcggcgcgct ggcgcagctg cgcgagctgg tgatcacgac caacggctcg
      661 cagctcgaac gctacgccgc cgaactgaag gccgccggcg tgcgtcgcct caacatcagc
      721 ctcgacagcc tgcgcccgga gcgcttccgg cgcatcacgc gcaccggcga tctcgaggtc
      781 gtgctgcgcg gcatccgcgc cgcgcaggcc gccggcttcg agcgcaccaa gctcaacgcc
      841 gtcgtgctca agcaccgcaa ccacgacgag gtcgtcgatc tcgtgcgctt cgcctgcgac
      901 gaggggctcg acctctcctt catcgaggaa atgccgctcg gcgtcatcgg cgaccatgac
      961 cgcgccgagg cctattactc gtccgacgac atccgccgcg acctcgccgg ccacttcgac
     1021 ctgatcccga ccaccgagca gaccggcggg ccgtcgaagt actaccgcgt cggcggcagt
     1081 gcgagccgca tcggcttcat ctcgccgcac agccacaact tctgcggcga ctgcaaccgc
     1141 gtgcgcgtgt cggccgaggg ccagctgctg ctctgcctcg gtcaggagca ttcggtcgac
     1201 ctgcgccacg tgctgcgcgc ccatcccggt gacccggagc gcctgaagca ggcgatcgtc
     1261 gaggcgatgc acatcaagcc caagggacac gatttcaacc tgaaggcgca gcccgtgatc
     1321 ctgcgccaca tgaacatgac gggcggctga acccgccgca gcgtgccggc aaccgccgtg
     1381 aatgcggtga attgccgatc cgagtaccac tggaccatca caaaccatca agagagagag
     1441 ggcctgtcga tgcaacgatc cctgtacatc gacccgcaga agtgcaccgg atgcctgcag
     1501 tgcgagatgg cctgctccta cgagaacgag caggccttca atccctcgaa gtcgcgcatc
     1561 aaggtgttcg cgttccacga ggaaggccgc ttcgtgccct acacctgcac gcagtgcgcc
     1621 gaagcctggt gcctgcatgc ctgcccggtc gaagccatca ccaagaaccc ggccaccggc
     1681 gcgatggagg tcaacgagaa gacctgcgtc ggctgcaagg tctgcacgat cgcctgtccg
     1741 ttcggcaccg tgaactacaa caccgccacc ggcaaggtca tcaagtgcga cctctgccag
     1801 ggcgacccgg cctgcgccaa ggcctgcccg accggcgcga tcacctacgt ggattcgagc
     1861 tggaccggac tcgacaagat gcgtgcctgg gccgcgaagg ccaacacggc tgcctgagcg
     1921 caggggagac tgagagatgg gctggaacaa caaggttttg cgcgtcaacc tgagcacggg
     1981 ctcgatgtcg gtcgagccgc tgcgcatgga ctgggccagg gactacctcg gccagcgcgg
     2041 tctggcgacc aagtatctgg tcgaggagac cgatccgaag gtcgatccgc tgtcgcccga
     2101 caacaagatg atcatgacca ccggtccgct gaccggcacc gaggcctcga ccagcggtcg
     2161 ctactcggtg gtcaccaagg gcccgctgac caacgcgatc gcctgctcga actccggcgg
     2221 cttcttcggc gccgagctca agtacgccgg ctgggacatg atcattttcg aaggcaggtc
     2281 gccgaagccg gtgtacctgt cgatcgtcga tgacaaggcc gagctgctcg acgccgccga
     2341 gatctggggc aagtcggtct gggagaccga gcactgggtg cacaagaagc accacgatcc
     2401 gctggtgcgc gtcgcgacca tcggtgccgg tgccgagaag ggcgtgctgt acgcggcgat
     2461 cgtcaacgac ctgcaccggg cggccggccg ctccggcgtc ggcgcggtca tggggtcgaa
     2521 gaacctcaag gccgtcgtcg tgcgcggcac gatcggcatg aacaaggtca aggatccggt
     2581 cgcgttccac gccgcctccg aagccgccaa gaaggtgctg gccgacaacg ccgtcaccgg
     2641 cgccggcctg ccgaagtacg gcacgcaggt gctgatgaac gtcatcaacg agatcggtgc
     2701 gatgccgacc cgcaactggc gcgaagtcca gttcgagggc gcctcggcga tctccggcga
     2761 ggccatgtac gagccgcgcc agagcgacgg caagccgaac ctggtgcgca acggcgcctg
     2821 cttcggctgc acgatcgcct gcggccgcat ctcgaccatc gacccgacgc acttctcggt
     2881 gcagggcgag ggcaaggagc gctacaagca cgcctccggc ggcctcgaat acgaagccgc
     2941 gtgggcgctc ggctccgaca ccggcatcga tgacctcgat gcgctgacct acgcgaactt
     3001 cctctgcaac gagcacggcc tcgatccgat ctccttcggt gcgaccgtcg ccgcggcgat
     3061 ggagctgtac gaagtcggcg cgctgaccaa ggagcagacg ggcggcatcg agttcaagtt
     3121 cggcaacgcc gaggcgctct gcaaggcggc cgagctgacc ggcaagggcg agggcttcgg
     3181 cctcgaactc ggcctcggct cgcggcgcct gtgcgagaag tacggccatc cggaattctc
     3241 gatgaccgtc aagagccagg aattcccggc ctacgacgcg cgcggcatcc agggcatggg
     3301 cctgacctat gcgacctcga accgcggcgc ctgccacctg cgcagctaca cggtcgcctc
     3361 cgaggtgctc ggcatcccgg tcaagaccga tccgctggtc accgacggca agccggaact
     3421 ggtcaaggcc ttccaggacg cgaccgccgt cgtcgattcg accggcctgt gcgtgttcac
     3481 gaccttcgcg tggacgctcg cggacttcca gccgcagatc caggccgcct gcgaaggcga
     3541 ctggagcatg gataagctca acgaggtcgg cgagcgcatc tggaacctgg agcgccagta
     3601 caacctcgat gccggcctga ccgctgccga tgacacgctg ccgaagcgcc tgctgaccga
     3661 agccgcaaag accggcccgg ctgccggccg cgtcaacgat ctcggcaaga tgatgccggt
     3721 gtactacgag ctgcgcggct ggaccgccga cggcaagctg aagcccgaga cgcgcaagcg
     3781 gctcggcgtc tgagacgcca gcaccctgcg caacggcccg gccaatcgcc gggccgttcg
     3841 ttcgaacggc ttgcgaggaa tactccatga tccaccgcta cgtcatcgtc ggtgccggcc
     3901 cggccggcgt catcgccgcc gaaaccctgc gcacgaccga tcccgacgcc gacatcacgc
     3961 tgatcggcga cgaacccgaa cgcccgtact cgcgcatggc gattccctac ctgctgatcg
     4021 agaagatcgg cgaggaaggc acgcacctgc gcaagggcaa cgatcactac gattccaagg
     4081 gcatccgcgt cctgcgcgac cgggtcgaga aggtcgatgc caaaacgaag cgcctgacgc
     4141 tgaagtccgg cggcgcgatg gactacgaca agctgctggt ctgcaccggc tcgcgtgccg
     4201 tgcgtccgcc ggtgccgggt ttcgacctgc ccggcgtgca cacctgctgg acgctcgagg
     4261 acgcccgcaa catcgcccgg ctcgccgaga agggcgcgaa ggtggtgctg gtcggcgccg
     4321 gcttcgtcgg ctgcatcgtg ctcgaagccc tggcgctgcg cggtgtcgat ctgaccgtgc
     4381 tcgaaatggg cgaccgcatg gtgccgcgca tgatgagccc gatcgccggc ggcctgatca
     4441 agagctggtg cgagaagaag ggcgtgcgcg tgatcacctc gacgcgcgtg accggcatca
     4501 ccgaaaacgg cggcctgatg aacaagctgg tcaacacgct gaccggcaag ccgacgcgcg
     4561 cgctgaaggt cacgctgaac accggcgaaa ccctcgatgc cgatctggtc gtctcggccg
     4621 ccggtgtcgc cccgaacatc gagttcctgc agggctgcgg gctggagatc gagcgcggca
     4681 tcctgatcga tcactacatg cagaccaacg tcccggacat ctatgcggcc ggcgacgtgg
     4741 cgcagggccg cgacttctcg accggtggtc tcgaagtgca tgcgatccag ccgaccgcct
     4801 ccgagcacgg ccgcaccgcc ggcctgaaca tggccggggt gccgacgtat taccgcggca
     4861 gcttcaacat gaacgtgctc gacaccctcg gcctgatctc cagctccttc gggctgtgga
     4921 tgggcgtcga ggggggcgat cgcgtcgaga tgtgcgagcc cgatcgcttc cgctacctgc
     4981 agctgcagtt cgatggtgac atcatggtcg gcgcgacctc gctcggcctg acgcagcacg
     5041 tcggcgtgct gcgcgggctg atccagggca aggtgccgct cggcccgtgg aaggcgaagc
     5101 tcatggatga cccgacgcgg atcatggagg cgtatctcgc ctgcaacttc gccgcccgcg
     5161 tggcgcccaa ggctgcctga gaccgacgat gcagatcacg ctgaagctgt atgcgacgct
     5221 gaccccgcac ctgcccgccg gcgcccggcg caacgtcgcg acgctggaag tcgcggacgg
     5281 tgccagcgtc gccggcgtca tcgacgatct gcatctgccg ccggccctcg tgcatctggt
     5341 gctggtcaac ggcagcttcg tcggccccga ggaccgggcc gcgcgcgtgc tgcgcgccgg
     5401 cgacgcgctc gcggtctggc cgccggtcgc cggtggctga gtcttcggcc ctgctgtggc
     5461 agggggagat ggcgctcgac cggcgcgagt tcctgcgcgt gctgccggcc gcctgtgccg
     5521 gccggccgca cgcgcaggcc ggcgatgcga tccgggtcga gctcggtgcc ggtgcaaggc
     5581 tgcgcatcga actcggcccg acccgcgagc ggcgcatcgc gctgctcgcg ctgccggcca
     5641 ccgtcatcac catcctcgcc gacggtccgg tgcccgcggc actggcgagt gccttcctgc
     5701 gcgatttcga gaacgcctac cggcgcggcg gcggctgaag gttcacttca gccgcacttc
     5761 gacgtggttg ggccagacct cgacccgcac cagcggcgtg tcctcgacga tgatccgttc
     5821 ggtcgtgcgg tccaggaagc cgtcggcgag caggcgttcg agccgggcgc ggttgtcggc
     5881 cgcggcgaag aagcggtcgg tttcgtcctc cggatcgcgt acgccgtagc ggtgcaggtt
     5941 ggcgcgatcc tggcggatga tcgccgcagc cgagcgcagg cgctcgccgc tgctgttgta
     6001 gtgatcggcc gcactcaggc gcgcgacgta gacctccagg ggttcgccgg cgagggccgg
     6061 cagcgacggc agcagggccg gcagcagggc cggcagcacg gcgatccagc gaaggcgggc
     6121 aggcagcagg ttcatgtcgg gaaagctccg ctcgggtcaa gggcagcggc cgcacatgcc
     6181 ggccggcggt ttcagaaaat ccccagttcc agccccgcag ccagcgccgc gccgagttcg
     6241 tgcgcgcgtt gcagcgcctc gtcgtccggc tcgccgcgca cgatcagcgc ctcggccact
     6301 tccttccagc catagccgcg ggcgatgcgt ccgatctcgc gcaccgcgcc gctgccgtcg
     6361 ttgccgcagc tgacgaacac cgcatacggc agcccgacgg tacggccctc ggccgggtag
     6421 taggtacggt cgaagaagtc cttcaggagc ccggacatgt agccgaagtt ctcgggcgtg
     6481 ccgagcagca ggccgtcgca gccgagcaga tcgtcgaggc cggcctcggg cgcacgcctg
     6541 agcagcgttt cgatgccgcc gcattcgcgt gcgccggccg cgacggcagc ggcgaggcgc
     6601 gccgtgcggc cgctctggct gtggaacacg atcagcaggc gccgggggcg ggcgggagtc
     6661 acggcgggag tccggcacag ggcagggacg tggagcgtag cgccgtccgg cccggctgtc
     6721 ccggaggccg ggttcaggcc ggcgtccggc agcgattcgc ccatgccatc gctgcggcat
     6781 ttccgcggcg gccggtccgg ccggatatag ccggatgccc atggacagcg ccgcaaatac
     6841 ccggattcat tcgcacgatt gcgcagcctg cggattcgtc cggcaggaag tgccggtttt
     6901 ttgttatcgg ttatcgtcac gaacatcccc tgaatcaggt gccggaattc cggcgcttct
     6961 gataattgct gtgagatgcc ggtcagtact ggtgtcggct gctggcacga atccgtgcgg
     7021 tggtcacaat gcggcatttc gtcgacggtg cagtcctcaa ggaattgccg gagccgtcga
     7081 aattcagatt gctgaacatt catcgcccca gggacatgac gatgtcgaaa accattctca
     7141 tcatcgatga ctcgccgtcc ttgcgccaga tcgtggccta cacgctgacc caggccggcc
     7201 acgcggtcgt cgaggctgcg agcggcgacg aggccttgca gcggctcgac gggcgcgtgc
     7261 tgaacctggt catctgcgac ctgcacatgc cgggcatcaa cggcctggag ttcgtcgcga
     7321 aactcaaggc ccgccacgaa tatcgcttcg tgccggtgct gatgctgacc accgaggcga
     7381 gcgacacctg ccgcgaggcc gggcgtgccg ccggggccaa ggcctggatg gtgaagccct
     7441 tccagccggc ggcgctgtgc gcggcggtca cgcggctgct ggccgcctga gccacgccat
     7501 gctgcacatc gacggcaatg gcaggcggct gacgctggcc gggcgattca cgatctatca
     7561 cgtcgcggaa ctgctcgcgt atctgcgcgg cctggaactg gcgccgatgc tggaaatcga
     7621 cctgtccggc gtgaccgatt tcgacagtgc cggcgtgcaa ttgctgctgg ccctgcagcg
     7681 ggaacagcag cagcacggcc gcgcattacg cctgctccag cacagtgctg cggtgctcga
     7741 cgtgttcacg ctgtatgagc tgtccgcgca tttcggggat ccggtcctgc tgccggccgg
     7801 cggtggcgca tgaacctgcc gctgcaggcc gtgatgcaga ccttctgcat cgaggcccgc
     7861 gaacggattg cggagctcga agccgggctg ctgaccctcg aacaggcgcc ctccgatacc
     7921 gaggccctga gtgcggtgtt ccgcgccgca cacacgatca agggctcggc cggcatgctc
     7981 gggctggatg cggtttcggc attcacgcac ggcttcgagg acgtgctcga tgcgctgagg
     8041 accggcgcgc gccgcttcga gcacaggctc ggcgaactgc tgctgctcgc cgccgatcat
     8101 ctggcgctgc tgctcgatgc cgccgaggcc ggcgaagtgc cggatgcggc gctgcaggcc
     8161 gccggcacgg cgctgctgca ggcgctgcac gaagagccgc aggcatcggc ggtccctgcg
     8221 ctcccgccgg caccggcccc gatgccggtc gagcgcgagc ggccggcggc cgaaaccggc
     8281 gggccggagt tctggcatct gtcggtgcgc ttcgggccgg atgcgctgcg caacggcttc
     8341 gacccgctcg gctgcctgcg ctacctgcac acgctcggac gcgtgctgcg cgtcgtgacg
     8401 ctgaccgatg cgctgccgga ctgggccggc ttcgatgccg agacctgctt cctcggcttc
     8461 gagatcgacc tcgattcgtc gcacgagcgc gagacgatcg ccgcgacctt cgagttcgtc
     8521 gccgatgact gcaccttgca catcctgccg ccgaacagcc aggccgcgca gtatctggac
     8581 ctgatccgcg cgctgcccga ggacgacctg tcgatcggcg agatcctcgt gcgcagcggc
     8641 tgcctgagcc gcctcgaact cgaacagggg ctgcgcgcgc aggcgcaggc ggcggccggc
     8701 agcaagcgcc tcggtgaact gctggtcgac cggcactgcg tggacgccga tgtcgtccgg
     8761 gcggcccttg agcgtcaggc gcacggccgt acccggcgca ccgccgaagc gcacacgctg
     8821 cgcgtgcagg cgccgcggct cgatgagctg atcgagcgca tcggcgaact cgtgaccgtc
     8881 ggggccggcg cgacgctgct cgcgcgcgaa ctcggcctgc cggcgctcga tgagctggcg
     8941 acgacgctga accggctggt ttccggcatc cgcgacaacg cgctgcagct gcgcatggtg
     9001 ccgatcggcg agaccttcgc gcgctttccg cgcgtcgtgc gcgacctcgg ccaggaactc
     9061 ggcaaggaca tcgatcttga gctctgcggc gaggacaccg aactcgacaa ggccatcgtc
     9121 gagccgctgg tcgatccgct gatgcacctg gtgcgcaacg ccgtcgatca cggcatcgag
     9181 ccgcttgcgg tgcgccgtgc gcgcggcaag cccggcgtcg gtcgcgtgcg gctgtcggcc
     9241 cgccacgagg gcgcgaacat gctgatcgag gtcgccgatg acggtggcgg cctggatggc
     9301 gagcgcatcc gcagcaaggc cgtcgccgcc ggcctgatcg gtgccggcga gcacctcgat
     9361 gcacaggggc tggcaaggct gatcttcgcg ccgggactgt cgacgcgcga cacggtcacg
     9421 gatgtctcgg gccgcggtgt cggcctggag gtcgtgcagc gcaacatcga ggcgctgcgc
     9481 ggcagcatcg aaatcgaatc cgaagccggg gccggcacga ccgtgcgcct gcgcctgccg
     9541 ctgacgctgg cgatcatcga cggcttcctg atccgctgcg ccggcacgag cttcgtgctg
     9601 ccgctcgacc aggtcgagga atgcctggat ttcgacgttg cggcgctgca tgccccgccc
     9661 cgccagctcg acctgcgcgg caggccgctg ccgctgatcc gcctgcatga attcttcggc
     9721 ctcggcacgc cgtgcggccg gcgcgccagt gtcgtggtcg tgcgctgcgg cgagcgccgc
     9781 gccggcctgc tggtcgatga actgctcggc gaattccaga ccgtcatcaa gccgctcgga
     9841 ccgctgttcg agcacctgcg cgcactcggc ggcacgacga tcctcggcag cggcgaagtc
     9901 gcgctgatcg tcgatgtgcc ggcgctgttc gcgctggcag cggcgcgcac gccgcc
//
LOCUS       DAOQIE010000130         9936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3452_length_9936_cov_6.726748, whole genome shotgun sequence.
ACCESSION   DAOQIE010000130 DAOQIE010000000
VERSION     DAOQIE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3452_length_9936_cov_6.726748"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1206
                     /locus_tag="PK144_09535"
     CDS             226..1206
                     /locus_tag="PK144_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNF66009.1"
                     /translation="MSASFLRRQHPGRGACLGLLPVLCLVSVSDSAGALDNPLDYDGS
                     HFLGLYVPLMLASLVLAMLLRRWLRLPGPDPRHQPRLDVYETAYLAGGAGRTVDTALA
                     VMALRGEIGVSPDGLRLQACGRPQLRDPIERKVFGALTLEGRPPGLHRRVRPVLCAEL
                     DARLRPQGLLPTPVQAAMVRLLPAACTGSVLLLGLLKLAIGLSRHRPVGFLVVCCLVL
                     GIATLVLLLKAVPRSRHGDAVLKQLRSGLSARTVESRQDPRLALVMALFGVAAVAHLL
                     PEDLAEQLAPAGSGGGDSGSGSDGSDSGGGDSGGDSGGGGCGGCGGGGGD"
     gene            1360..3120
                     /locus_tag="PK144_09540"
     CDS             1360..3120
                     /locus_tag="PK144_09540"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNF66010.1"
                     /translation="MREPRELHFDTRDGSALFYRHWPALDGPGEQALLLLHRGHEHSG
                     RLQHVVDELDLPGFAVFAWDARGHGRSPGPRGHCPGIATATGDLDTFVRHLRDRHGIA
                     PENLVVIAQSVGAVIAATWAHDYAPPVRALVLAAPAFKVKLYVPLARPALRLRYALGG
                     NFEVQSYVKAKFLTHDPARIASYDADPLITRAIAVNMLLGLYEAGERVVEDAAAITLP
                     VLVLVSGADFVVHRAPQRRFFERLGSKVKAYHELPGFYHDTFGELRRSEAIGLVRAFV
                     RKCFDAPFERPDLRTADRHGHTFDEYRRLQAPLPEGSWKIPYYAAVRAGLALGARLAE
                     SIRIGHETGFDSGSTLDAVYRNRPAGFGPPGRLIDRLHLDSAGWAGIRIRKQHVEALL
                     ADAVRALRRAGRPVRIVDIAAGHGRYVLDGLATLPEPLEAADSVLLRDYSPINVAAGR
                     QLIAARGLAATVRFEQGDAFDRDALAALTPKPTVAIVSGLYELYPDNAPLAASLSGLA
                     AALEAGGFLIYTGQPWHPQLELIARALASHRDGAPWVMRRRTQGELDQLVAAAGFDKC
                     TQLIDDAGIFTVSLAVRRAS"
     gene            3117..4466
                     /locus_tag="PK144_09545"
     CDS             3117..4466
                     /locus_tag="PK144_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HNF66011.1"
                     /translation="MSAAGSATGSTAGAHEARPWPRALLWLAGLGPLFFLSYGAANWL
                     ASRRAGVGSLVFDWEHAIPFWPWTILPYWSIDLLYALSLFVCTTRRELDAHGRRLLTA
                     QLVAVSAFVLVPLRFTFERPPVDGLPGALYALLGSFDLPYNQAPSLHIALLLILWVRF
                     DAHTGRRWRWLLHVWFALIGVSVLTTWQHHFIDVPSGMWLGLFAIALFPTDGTLPALS
                     RPDARRIALAARYGLGAAACAVAGAWAGGLGWLLLWPAGALALVALIYLAGDPRLFRK
                     GHDGRMNPAMLVLFGPYLFGAWLNARLWTLVHPEPSEIAPGVWLGRVPRRRERERAGF
                     DALLDLSAELPVDRRGVASRGVPMLDLLPPEPAQIEAAVQALEGLHGGRHATLVCCAL
                     GYSRSALVSAAWLFASGRADSLAAAVGQVRKSRPRVVLKPAHLARLNEWAGTRGLPT"
     gene            4463..4903
                     /locus_tag="PK144_09550"
     CDS             4463..4903
                     /locus_tag="PK144_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66012.1"
                     /translation="MSDMSDINPDPAMAVAVTAALLDAGRVAAAIGHGSALLAGAALL
                     ATLPASGTTRLCCGLALALWPVSGWLALRVALDARLFATLDADEASFTRFDACLPALG
                     LYPSQPGPRPLPLRCRGALRLWRSLLAVAGVQFALVLVAALARH"
     gene            complement(4925..6568)
                     /locus_tag="PK144_09555"
     CDS             complement(4925..6568)
                     /locus_tag="PK144_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HNF66013.1"
                     /translation="MSISDPGRVPAAQAATRLNLGQLLCNASRQFPQQLIVHGERRFT
                     YAEFGRRVHRLAAVLDALGVRAGETVAVLDWDSHRYLEAFLAVPMLGAVLHTVNVRLS
                     PEQIAWTIEHAADDVILVHEDFLPLLDAIRPRLAHVRRFVLLCDHAGGEALGGLPFAG
                     EYEALLAAATGDFAFPEVAEDARATTFYTTGTTGLPKGVFFSHRQLVLHTLGVRSTLS
                     GTGQGHIGADDVYMPITPMFHVHAWGMPLVALSMGLKQVYPGRYEPAKLLRLIVREGV
                     SFSHCVPAILQMLLNCPDGREMDLDGWKVLIGGSALPESLAIAALERGIDVFGGYGMS
                     ETCPILCVAQLTPEVLRRPFAEQVRIRCKAGRAAPLVQLRIVDDEMNDVAHDGIAQGE
                     VIARAPWLTEGYLHNPEAGAALWRGGWLHTGDIGTIDADGYLKITDRLKDVIKTGGEW
                     VSTLMLEDLILRLHEVAECAVVGIPDPRWDERPLALVVLRGGASLDPAMIRAHLAGFV
                     AEGLIPKYALPERIEQVEAIPKTSVGKLDKKAIRAQYAG"
     gene            6882..9461
                     /gene="acnB"
                     /locus_tag="PK144_09560"
     CDS             6882..9461
                     /gene="acnB"
                     /locus_tag="PK144_09560"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNF66014.1"
                     /translation="MLDAYRKHAEERAAQGIPALPLSAQQTADLVELLKNPPAGEEAF
                     LLDLLTHRVPAGVDQAAYVKAAFLAAVAKGEASSPLLNRVRATELLGTMLGGYNIQPL
                     IDLLDDAECAAAAGKALSHTLLMFDYKNAVKDKADAGNATAAAVLKSWADAEWFTSRP
                     KVPESVTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADQPGSVG
                     PIKQLAALQAKGNPIAYVGDVVGTGSSRKSATNSVLWWTGEDIPYVPNKRFGGYCFGS
                     KIAPIFFNTMEDAGALPVEVDVSKMDMGDVVELRPYDGKALKNGEVIAEFSFKSDVLL
                     DEVQAGGRINLIIGRGLTDWAREQLGLGASPVFRRPAAQVESSQGFSLAQKMVGKACG
                     VTGIRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPV
                     DVQTHHTLPDFISSRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPA
                     GSGLVAFAAATGSMPLDMPESVLVRFKGTMQPGITLRDLVNAIPLYAIKAGLLTVAKQ
                     GKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCAIALNKEPIAEYLNSNITL
                     MKWMIANGYSDAKTLERRIKGMEAWLAAPELMKADADAEYAAVIDIDLADIKEPILAC
                     PNDPDDVKLLSEVAGDKIDEVFIGSCMTNIGHFRAAGKLLEGKSDIPVRLWIAPPTKM
                     DAMILSEEGYYGILGRSGARMEMPGCSLCMGNQAQIRKGSTAISTSTRNFPNRLGIDT
                     RVYLGSAELASICAMLGKIPTVDEYMANIGVINAKAGEIYRYMNFDQIAEFSEVAGKV
                     EVA"
BASE COUNT         1523 a         3547 c         3309 g         1557 t
ORIGIN      
        1 gttcaaacgc tcgcgggcgc ggctgacacg caggagcatt ccgtcgatga gatggatctg
       61 ctgctggatg gcggcgagcg aggtggcaaa atcgtcgcgc tgcttcttgg acatgagcag
      121 gctgatcttc gggcggctgt tgcgcagcct gcgccggacg ctggcccgct tcggccttgc
      181 ctctgaaccc gcaccaggtc ctgtccgcga ccccgggaga aagtcatgag cgcatcgttc
      241 ctgcgccgcc agcatccggg tcgcggcgcc tgcctcggcc tgctgccggt gctgtgtctc
      301 gtcagcgtca gcgacagcgc cggcgcactc gacaacccgc tcgactacga cggcagccac
      361 ttcctcggac tctacgtgcc gctgatgctg gcgagcctcg tgctggccat gctgctgcgc
      421 cgctggctgc gtctgcccgg tcccgatccg cgccaccagc cccggctcga tgtctacgaa
      481 accgcgtatc tcgccggcgg cgccgggcgc acggtcgata cggccctggc cgtgatggcg
      541 ctgcgcggcg agatcggcgt cagccccgac ggcctgcgcc tgcaggcctg cggccggccg
      601 cagctgcgcg acccgatcga acgcaaggtg ttcggggccc tgacgctgga gggccggccg
      661 ccgggcctgc accgccgtgt ccgccccgtg ctttgcgccg aactcgatgc acggctgcgc
      721 ccgcagggac tgctgccgac gccggtgcag gcggcgatgg tccggctgct gccggccgcc
      781 tgcaccggat cggtgctgct gctcgggctg ctgaagctcg cgatcggtct gtcacggcat
      841 cgaccggtcg gcttcctcgt cgtctgctgc ctcgtgctcg gcatcgcaac gctggtgctg
      901 ctgctgaagg ccgtgccgcg cagccgccac ggcgacgccg tgctgaaaca gctgcgcagc
      961 ggcctgtcgg cccgcaccgt cgaatcgcgg caggatccgc gcctcgcctt ggtcatggca
     1021 ctgtttggcg tggcggccgt cgcgcatctg ctgcccgagg acctcgccga acaactggcg
     1081 ccggccggtt ccggcggcgg cgattcgggc agtggatcgg acggcagcga cagcggcggc
     1141 ggggacagcg gtggcgacag cggcggtggc ggctgcgggg gctgtggcgg cggcggtggc
     1201 gactgaagcg tgagcctgcg ggctggcccc gggcacaggc cggctgatcc gccaccgcac
     1261 acaccggcat tgcagcaagt ccggcaaccg gcagccatgc ttgtcgggcg cggccggccc
     1321 ccgcgcattc ccccgccccg cccgacgacg gagccctcga tgcgcgaacc ccgggaactg
     1381 cacttcgaca cgcgtgacgg cagcgcgctg ttctaccggc actggccggc cctcgacggc
     1441 cccggcgagc aggcgctgct gctgctgcac cgcggccacg agcattcggg ccggctgcag
     1501 cacgtggtcg atgagctcga tctgccgggt ttcgcggtgt tcgcctggga tgcccgcgga
     1561 cacggccgtt cgccggggcc acgcggtcat tgccccggca tcgccaccgc caccggcgat
     1621 ctcgacacct tcgtgcgcca cctccgcgac cggcacggca tcgcgcccga aaacctcgtc
     1681 gtgatcgcgc agagcgtcgg tgccgtgatc gccgcgacct gggcgcacga ctacgcgccg
     1741 ccggtgcgtg cgctggtgct cgcggccccg gccttcaagg tcaagctgta cgtgccgctg
     1801 gcgcgcccgg cgctgcggct gcgttacgcg ctcggcggca acttcgaggt gcagtcgtac
     1861 gtgaaggcga agttcctgac ccacgatccg gcgcgcatcg ccagctacga cgccgatccg
     1921 ctgatcacgc gcgcgatcgc cgtcaacatg ctgctcgggc tgtacgaggc cggcgagcgc
     1981 gtggtcgagg acgccgcggc gatcacgctg ccggtgctcg tgctggtctc gggcgccgac
     2041 ttcgtcgtgc accgcgcgcc gcagcggcgc ttcttcgagc ggctcggctc gaaggtgaag
     2101 gcgtatcacg agctgccggg cttctatcac gacaccttcg gcgaactgcg ccgcagcgag
     2161 gcgatcgggc tggtgcgcgc cttcgtgcgc aagtgcttcg atgcgccgtt cgagcggccg
     2221 gacctgcgca cggccgaccg ccacggccat accttcgacg aataccggcg gctgcaggcg
     2281 ccgctgccgg aaggctcgtg gaagattccg tactacgcgg cggtgcgcgc cggcctcgcg
     2341 ctcggcgccc ggctcgccga atcgatccgc atcggccacg agaccggctt cgactcgggc
     2401 tcgacgctcg atgccgtcta ccgcaaccgc ccggccggct tcggcccgcc cggacggctg
     2461 atcgaccggc tgcacctcga ttcggccggc tgggccggca tccgcatccg taagcagcac
     2521 gtcgaggcgc tgctcgccga tgccgtgcgc gcgctgcgcc gcgcgggccg cccggtgcgc
     2581 atcgtcgaca tcgccgccgg ccacggccgc tacgtgctcg atggcctcgc gacgctgccc
     2641 gagccgctcg aagcggcgga ttcggtgctg ctgcgcgact acagcccgat caacgtcgcc
     2701 gccggccggc agctgatcgc cgcacgcggg ctggcggcca cggtgcgctt cgagcagggc
     2761 gacgccttcg atcgcgacgc gctcgcggcg ctgacaccga aacccaccgt cgcgatcgtc
     2821 tccggcctct acgagctgta cccggacaac gcgccgctgg cagcgtcgct gtcgggcctc
     2881 gccgcggcgc tcgaagccgg cggcttcctg atctacaccg gccagccctg gcatccgcag
     2941 ctcgaactga tcgcacgcgc gctggcgagc caccgcgacg gcgcaccgtg ggtgatgcgc
     3001 cggcgcacgc agggcgagct cgaccagctc gtcgccgcgg ccggcttcga caagtgcacg
     3061 cagctcatcg acgatgccgg catcttcacg gtgtcgctgg ccgtgcggag ggcgtcatga
     3121 gtgccgcagg aagtgccacc ggaagtaccg ccggggcgca cgaagcccgc ccgtggccgc
     3181 gggcgctgct ctggctcgcc ggcctcgggc cgctgttctt cctgagctac ggcgctgcca
     3241 actggctcgc gagccggcgc gccggcgtcg gcagcctggt gttcgactgg gaacacgcga
     3301 tccccttctg gccgtggacg atcctgccgt actggtcgat cgacctgctc tacgcgctgt
     3361 cgctgttcgt ctgcacgacg cggcgcgagc tcgacgcgca cggccggcgg ctgctgacgg
     3421 cgcagctcgt cgcggtcagc gccttcgtgc tggtgccgct gcgcttcacg ttcgagcgcc
     3481 cgccggtcga cggcctgccc ggggcgctgt acgcgctgct cggcagcttc gacctgccgt
     3541 acaaccaggc gccgtcgctg cacatcgcgc tgctgctgat cctgtgggtg cgcttcgatg
     3601 cccacaccgg ccggcgctgg cgctggctgc tgcacgtctg gttcgcgctg atcggcgtgt
     3661 cggtgctgac gacctggcag catcacttca tcgacgtgcc gagcggcatg tggctcggcc
     3721 tgttcgcgat cgcgctgttc ccgaccgacg gcacgctgcc ggcgctgagc cgccccgatg
     3781 cccggcgcat cgcgctcgcg gcgcgctacg gcctcggggc cgcggcctgt gccgtggccg
     3841 gcgcctgggc cggcgggctc ggctggctgc tgctgtggcc ggccggggcg ctggcgctgg
     3901 tggcactgat ctatctcgcc ggcgatccgc ggctgttccg caagggccac gacgggcgca
     3961 tgaacccggc gatgctggtg ctgttcgggc cgtacctgtt cggcgcctgg ctgaacgcgc
     4021 ggctctggac gctggtgcat cccgagccgt ccgagatcgc tcccggcgtc tggctcgggc
     4081 gcgtgccgcg ccggcgcgag cgcgagcgcg ccggtttcga tgcgctgctc gacctgagcg
     4141 ccgaactgcc ggtcgaccgg cgcggcgtcg ccagccgcgg cgtgccgatg ctcgacctgc
     4201 tgccgccgga gccggcgcag atcgaggccg ccgtgcaggc gctcgaaggc ctgcacggcg
     4261 gccggcacgc gacgctggtc tgctgcgcgc tcggctattc gcgcagcgcc ctggtcagcg
     4321 ccgcgtggct gttcgcgagc ggccgcgccg acagcctcgc ggctgccgtc gggcaggtgc
     4381 gcaagtcccg cccgcgcgtg gtgctgaaac ccgcgcatct ggcgaggctg aacgaatggg
     4441 cgggcacccg ggggctgccg acatgagcga catgagcgac atcaatcccg acccggcaat
     4501 ggccgtggcc gtcaccgccg cgctgctcga tgccggccgg gtcgctgccg ccatcggcca
     4561 cggcagcgcg ctgctcgccg gtgccgcgct gctcgccacg ctgccggcca gcggcacgac
     4621 gcggctctgc tgcggactgg cgctggcgct gtggccggtg agcggctggc tggcgctgcg
     4681 cgtcgcgctc gatgcgcggc tgttcgcaac gctcgatgcc gacgaggcga gcttcacgcg
     4741 cttcgatgcc tgcctgccgg cactcggcct gtatccgtcg cagcccggcc cgcgaccgct
     4801 gccgctgcgc tgtcgcggcg cactgcggct gtggcgcagc ctgctcgccg tcgccggcgt
     4861 gcagttcgcg ctggtgctgg tggccgccct cgcgcggcac tgaaacccgg ccggccggcc
     4921 gcgctcagcc ggcgtactgc gcgcggatcg ccttcttgtc gagcttgccg acgctggtct
     4981 tcgggatcgc ctcgacctgt tcgatgcgct cgggcagcgc gtacttcggg atcagccctt
     5041 cggcgacgaa accggccaga tgcgcgcgga tcatcgcggg gtccaggctg gccccgccgc
     5101 gtagcacgac cagcgccagc ggccgttcgt cccagcgcgg atcggggatg ccgaccaccg
     5161 cgcattcggc gacctcgtgc aggcgcagga tcaggtcttc gagcatcagc gtcgacaccc
     5221 actcgccgcc ggtcttgatg acatccttga ggcgatcggt gatcttcagg tagccatcgg
     5281 cgtcgatcgt gccgatgtcg ccggtgtgca gccagccgcc gcgccagagc gccgcgccgg
     5341 cctcgggatt gtgcagatag ccctcggtca gccacggcgc gcgggcgatc acctcgccct
     5401 gcgcgatgcc gtcgtgcgcc acgtcgttca tctcgtcatc gacgatgcgc agctgcacca
     5461 gcggcgcggc gcgcccggcc ttgcagcgga tgcgcacctg ctcggcgaag ggccggcgca
     5521 ggacctcggg cgtcagctgc gcaacgcaga ggatcggaca ggtctcggac atgccgtagc
     5581 cgccgaacac gtcgatgccg cgctccagcg ccgcgatcgc cagtgactcc ggcagcgccg
     5641 aaccgccgat cagcaccttc cagccgtcga gatccatctc gcggccgtcc gggcagttca
     5701 gcagcatctg caggatggcc ggcacgcagt gcgagaagct cacgccctcg cgcacgatca
     5761 gcctgagcag cttcgccggc tcgtaacggc ccggatacac ctgcttcagc cccatcgaca
     5821 gcgccaccag cggcatgccc caggcgtgca cgtggaacat cggcgtgatc ggcatgtaga
     5881 catcgtcggc gccgatgtgg ccctgcccgg tgccggaaag cgtcgagcgc acgccgagcg
     5941 tgtgcagcac cagctggcgg tggctgaaga acacgccctt cggcaggccg gtggtgccgg
     6001 tcgtgtagaa ggtcgtcgcg cgcgcatcct cggccacctc cgggaaggca aagtccccgg
     6061 tcgccgccgc cagcagcgcc tcgtactcgc cggcaaacgg caggccgccc agggcctcgc
     6121 ccccggcgtg atcgcagagc agcacgaagc ggcgcacgtg cgccagccgc gggcggatcg
     6181 catcgagcag cggcaggaag tcctcatgca cgaggatcac gtcgtccgcg gcatgctcga
     6241 tcgtccacgc gatctgctcg ggcgacaggc gcacgttcac cgtgtgcagc accgccccga
     6301 gcatcggcac cgcgaggaag gcttcgaggt agcggtgcga atcccagtcg agcaccgcga
     6361 ccgtctcgcc ggcgcgcacg ccgagggcat cgagcacggc agcgagccgg tgcacgcgcc
     6421 ggccgaactc ggcatacgtg aagcgccgct cgccgtgcac gatgagctgc tgcgggaact
     6481 ggcgcgaggc gttgcagagc agctggccga gattcaggcg cgtcgccgcc tgggcggccg
     6541 gcacgcggcc gggatcggaa atggacatgg tgtggagact cccctgctca tgacggccgg
     6601 tctgctgccg gtcctttgac cccgagtctg gcctgcgcgc tgcgcactgt cagccatcca
     6661 gcccccgggg aatgtccaca atgtcgcatg ccgcaacggc ttccggtttt ccgcgtgcgc
     6721 cgattccgtt tagactgccg cgatttttcc ggacaagcgg ccacagagcc gcagccggag
     6781 cgaggcgcag cacgaaccgc gcctcacatc gcttcatgga tcggggtttt ccagacccgc
     6841 tgcccgccgg cagctcatca cgcaacgagg gggtttaaac cgtgttagat gcctatcgca
     6901 aacacgccga ggaacgcgct gcccagggca ttccggccct gccgctgtcc gcccagcaga
     6961 ccgcggacct ggtcgaactc ctgaagaatc cgcccgcagg cgaagaagcc ttcctgctcg
     7021 acctgctgac ccaccgcgtg ccggccggcg tcgaccaggc cgcctacgtc aaggccgcct
     7081 tcctcgccgc cgtcgccaag ggcgaggcga gctcgccgct gctcaaccgc gtgcgcgcca
     7141 ccgagctgct gggcaccatg ctcggcggct acaacatcca gccgctgatc gacctgctcg
     7201 acgacgccga atgcgccgcc gccgccggca aggcgctgtc gcacacgctg ctgatgttcg
     7261 actacaagaa cgccgtcaag gacaaggccg atgccggcaa cgccaccgcc gcggccgtgc
     7321 tgaagtcctg ggccgacgcc gagtggttca cgagccgccc gaaggtcccg gagagcgtca
     7381 ccgtcaccgt attcaaggtc accggcgaaa ccaacaccga tgacctgtcc ccggctcccg
     7441 acgcctggtc gcgtccggac atcccgctgc acgcgctggc gatgctgaag aacccgcgtc
     7501 cgggcatcga agccgatcag cccggctcgg tcggtccgat caagcagctc gccgcgctgc
     7561 aggccaaggg caacccgatc gcctacgtcg gcgacgtggt cggcaccggc tccagccgca
     7621 agtccgcgac caactccgtg ctgtggtgga ccggcgagga catcccctac gtcccgaaca
     7681 agcgcttcgg cggctactgc ttcggcagca agatcgcccc gatcttcttc aacacgatgg
     7741 aagacgccgg cgcgctgccg gtcgaagtcg acgtgtcgaa gatggacatg ggcgacgtcg
     7801 tcgagctgcg cccctacgac ggcaaggcgc tgaagaacgg cgaagtcatc gccgagttca
     7861 gcttcaagtc cgacgtgctg ctcgatgaag tccaggccgg cggccgcatc aacctgatca
     7921 tcggccgcgg cctgaccgac tgggcgcgcg agcagctcgg cctcggcgcc tcgccggtgt
     7981 tccgtcgtcc ggccgcgcag gtcgaatcga gccagggctt ctcgctcgcg cagaagatgg
     8041 tcggcaaggc ctgcggcgtc accggcatcc gtccgggcac ctactgcgaa ccgaagatga
     8101 ccacggtcgg ctcgcaggac accaccggcc cgatgacccg cgacgagctg aaggacctcg
     8161 cctgcctcgg cttctcggcc gacatggtga tgcagtcgtt ctgccacacc gccgcctatc
     8221 cgaagccggt cgacgtgcag acgcaccaca cgctgccgga cttcatctcc agccgcggcg
     8281 gtgtcgcgct gcgcccgggc gacggcgtca tccactcgtg gctgaaccgc ctgctgctgc
     8341 cggataccgt cggcaccggc ggcgactcgc acacgcgctt cccgatcggc atcagcttcc
     8401 cggccggctc gggcctggtc gccttcgccg ccgccaccgg ctcgatgccg ctcgacatgc
     8461 cggaatcggt gctcgtgcgc ttcaagggca cgatgcagcc cggcatcacg ctgcgtgatc
     8521 tcgtcaacgc gatcccgctg tacgcgatca aggcggggct gctgaccgtc gccaagcagg
     8581 gcaagaagaa catcttctcg ggccgcatcc tcgagatcga aggtctgccg gatctcaagg
     8641 tcgagcaggc cttcgaactg tccgatgcct cggccgagcg ttccgccgcc ggctgcgcga
     8701 ttgccctgaa caaggagccg atcgccgaat acctgaactc gaacatcacg ctgatgaagt
     8761 ggatgatcgc gaacggctat tcggatgcga agacgctgga gcgccgcatc aagggcatgg
     8821 aagcgtggct cgccgcgccc gaactgatga aggccgatgc cgacgccgaa tacgcggccg
     8881 tcatcgacat cgacctcgcc gacatcaagg aaccgatcct cgcctgcccg aacgacccgg
     8941 acgacgtgaa gctgctgtcc gaagtcgccg gcgacaagat cgacgaagtc ttcatcggct
     9001 cgtgcatgac caacatcggt cacttccgcg ccgccggcaa gctgctcgaa ggcaagtccg
     9061 acatcccggt gcgcctgtgg atcgccccgc cgaccaagat ggacgcgatg atcctcagcg
     9121 aggaaggcta ttacggcatc ctcggccgca gcggcgcgcg catggaaatg ccgggctgct
     9181 cgctgtgcat gggcaaccag gcgcagatcc gcaagggctc gaccgccatc tcgacctcga
     9241 cccgcaactt cccgaaccgc ctcggcatcg acacccgcgt ctatctcggc tcggccgaac
     9301 tggcgtcgat ctgcgcgatg ctcggcaaga tcccgacggt cgacgagtac atggccaaca
     9361 tcggcgtcat caacgccaag gccggcgaaa tctaccgcta catgaacttc gatcagatcg
     9421 cggagttcag tgaggtggcg ggcaaggtgg aagtcgcctg aacgtcggat gatgtgatgt
     9481 gcgcgcaaag gattgcggga tgaaatgaaa aaggccccgg atcggaaggt tcggggcttt
     9541 ttactgcaac gacagcagac tctttgatag aaatgattgg aaaaataaaa aaagcactct
     9601 cgttcccaat cgactctgaa tcagtagaaa tacctgtcaa tacgattgtt aatattgaat
     9661 ttcttatgcc atttgaacaa gacaatggac gacactgcat gtataaactt atcatctcta
     9721 gcgattgcag cataattata tccgaattcg aattaaagat ttatgttgaa atactataaa
     9781 ccattataat accgtaaaaa gcatcttaac cacacgtaag agaagaaatt caaatcatga
     9841 aaaaatcatt attaatctca attacagtat tattaacccg acaaacaaac accaattaca
     9901 tccgctatcc ttcctgagca cctgttgatt gtttga
//
LOCUS       DAOQIE010000131         9900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3468_length_9900_cov_8.551143, whole genome shotgun sequence.
ACCESSION   DAOQIE010000131 DAOQIE010000000
VERSION     DAOQIE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3468_length_9900_cov_8.551143"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK144_09565"
     CDS             <1..375
                     /locus_tag="PK144_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66015.1"
                     /translation="SFRLPRAAAMTDAPTPAELRARLAGTPDTAGLPEALLERLAAED
                     PALRAQAAAELTELYNLTWREGQAPVDEAGFAAALTLVQVGWQAGALQWLRYTAPALI
                     GPAAVVLYADDEGRGFRACIEA"
     gene            540..1001
                     /locus_tag="PK144_09570"
     CDS             540..1001
                     /locus_tag="PK144_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66016.1"
                     /translation="MPSSVALLPIRARSLLLAVALGAAALPAAAGKYTDQVNILLDRV
                     DTAMAKRGFEQTHTPKIDKLRQGDSDSFSFTLKKGMSYKIVSVCDADCSDLDLVLRDE
                     GGNDIDNDDQRDDMPIVSVQPKWTGKFTLRVKMYSCNNNPCYYGIGIYGTR"
     gene            complement(1054..1242)
                     /locus_tag="PK144_09575"
     CDS             complement(1054..1242)
                     /locus_tag="PK144_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66017.1"
                     /translation="MNAVARCGLCLALAAGVSACAVVPAEPYGYGYGYGYRPAPVAVV
                     APPVVVGGWWHPYRYRRW"
     gene            complement(1239..1574)
                     /locus_tag="PK144_09580"
     CDS             complement(1239..1574)
                     /locus_tag="PK144_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66018.1"
                     /translation="MASRRIRAALLLAAAVAAPLTAAVAGEFVPVRRVVEEVIVTEAP
                     PPPREEVIIERPSPRHVWVAGHWQRVGNEWVWQNGHWLQPPRLGAGWVPGHWDQRPRG
                     WVWVPGHWA"
     gene            complement(1677..2414)
                     /locus_tag="PK144_09585"
     CDS             complement(1677..2414)
                     /locus_tag="PK144_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF66019.1"
                     /translation="MPAARADEGIARALLRAQPQRFAGQPLTATMFANVRARAHATLA
                     LAFALLALAALLTALIGDAPLSRHLSSRFWVAGQRSLLKWLTDAGLYAGYACFIALLV
                     LGWWRRQRLWFEVARVWLAAQLIGGLLVVRVLKMWIAHPRPDTGAGDAPLQWLGLTWN
                     AAYNSFPSGHSADIATSAFCLCLLLARPWQRAWVLGFAALIGLTRILLAKHWASDVLA
                     GSAVGLTVAALIAGFWLRRLATGDAPA"
     gene            complement(2395..3987)
                     /locus_tag="PK144_09590"
     CDS             complement(2395..3987)
                     /locus_tag="PK144_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF66020.1"
                     /translation="MMSPVAAPAAGRDAFWRDAALLVLALLAVHALNLAVHRDLWVQD
                     EARYGEVLREMLESGRWLVPHLNGFPYPDKPPVYFWLVAALGALTGQGELAFRLFTLL
                     STVAATFGTWVLGRRLAGPAAGRWAALCFVTLFGTLVVGQIARMDMTLCATCVWAWVA
                     LHAHETRRSRAALVGFWLASAAGVAIKGPIALLFTVLPALLRPLGARDWAGFAALKPL
                     HGLGLLVGGVLAWIAAVWLDGESAYLVTIWNEQLVGRAVNSWSHREPVWFYVTLLPVL
                     LMPWTGLAVSGARTFWQSEPIAARFVLACLLVALLAISAISGKLFIYLQPLLPLCAVL
                     AGVGCTTLLAQSRIGTEQALLPVVWFTLLAGGIGWGTHDVLGNNPRGFALATILGLCA
                     ILGFRLSRQPPRRWLIGWTGLSAVVSWVVFGALITLVNPLYSGKPLGEAIARLAPGSR
                     PVAIVHSTRGILNYYAGRLMTEVERTDIAAWYLLHPDALVVIQSAHLSYWFPEGKVPA
                     CTTDETYSVELKEYRVLAGCPR"
     gene            complement(3984..4574)
                     /locus_tag="PK144_09595"
     CDS             complement(3984..4574)
                     /locus_tag="PK144_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNF66021.1"
                     /translation="MPAAIESESALAATRAWVEHLVVGLNLCPFASLPFTKKTIRFAV
                     CDASQPAVIAERLAGELALLAEADPAIVQTTLLILPNAFADFLDFNDFLDVADALVEE
                     AGLAGVLQVASFHPGYQFADEAPDDVSHWTNRSPHPVLHLIREDDIERAAATHPDVDG
                     IPDANIAKLRTLGRPAIEALLERCRAAGEAARKPAE"
     gene            4824..5249
                     /locus_tag="PK144_09600"
     CDS             4824..5249
                     /locus_tag="PK144_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF66022.1"
                     /translation="MKIKDLLATKTGRPVTVAPTATIAEAVRAMHGNRVGAVLIPDAT
                     GCPLGIFTERDVVRLYAEGDDLTQVTVEQRMTCSVVLGKPDDSIDSVMSLMTERRFRH
                     LPVVDGGTLLGLVSIGDVVKAKLDEAAKEAETLREYIHS"
     gene            5308..5733
                     /locus_tag="PK144_09605"
     CDS             5308..5733
                     /locus_tag="PK144_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HNF66023.1"
                     /translation="MPPFWQTKSLAELSPQEWESLCDGCGRCCLHKLEDEDTGELFHT
                     RVACRLLDTHSCRCTRYANRRRWVPDCVRLTPADVPRFRWLPETCAYRRLAEGRPLPE
                     WHPLLTGDRESVHAAAASARGWCISERDGVDLHAHIIGR"
     gene            5818..6513
                     /locus_tag="PK144_09610"
     CDS             5818..6513
                     /locus_tag="PK144_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF66024.1"
                     /translation="MNLRCFTVGLFQVNAWLIEDPETGACVLIDTGESDEVASELAAL
                     TPRPDLRAILLTHAHFDHAGGLVPLQALYPQAETCLPRLERPMFDLLPRQGELFGAPQ
                     FSKPCGRIDREIDDDELVRVGALECRFVSTPGHTPGQGCWQVGGHLFVGDTIFAGSVG
                     RTDFPLSDPGLAQKSLAKLMTLPGELHVHCGHGPDTTLAAELKGNPYLAHVRRALGIK
                     EPRGGYARGGNWG"
     gene            6520..7341
                     /locus_tag="PK144_09615"
     CDS             6520..7341
                     /locus_tag="PK144_09615"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005893851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNF66025.1"
                     /translation="MCELLGMSANVPTDIRFSFTGLMQRGGHTGPHRDGWGIAFYGER
                     GARLFHDPCPSCESEVARLVQQHSIKSRIVISHIRRANRGRVCLENTHPFTRELWGRS
                     WVFAHNGELKGIKRRPCGPYEPIGSTDSEHAFCWLLGRIRERFPQPPRVPATLWRYVA
                     GLAAELNQLGIFNFLLSDSRWLYAHCSTKLAWLTRRAPFGEARLTDADMAVDFQGETT
                     PDDVVTVIATQPLTGNERWNLLAPGDFIVFDGGEPVWQHGVPAGCATPSAFLHPG"
     gene            complement(7364..7801)
                     /locus_tag="PK144_09620"
     CDS             complement(7364..7801)
                     /locus_tag="PK144_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF66026.1"
                     /translation="MLRRLAYVCLSVVLAGCASGLSERRARVLASAEALIGTPYRWGG
                     TDRSGLDCSGLVQLSYAEAGLRVPRTTTALLRDGRPAADPEPGDLLLFSENEPGKPTH
                     VGIYAGDGRMIHASSRGGRVLKSSLDTPYWRRNLIGCVRYLDG"
     gene            7909..8391
                     /locus_tag="PK144_09625"
     CDS             7909..8391
                     /locus_tag="PK144_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF66027.1"
                     /translation="MKAASGMVVSMHYTLTDDAGEVLDTSQGRGPFDYLHGHGNIIPG
                     LESRLEGAEPGFKDKVTVAPADGYGERNDQAVFDVPRDQFPPDEEIRVGMQVHGEGPQ
                     GVIAFTVVDVAEDSIRLDGNHPLAGKTLHFDVEILDIRAASEEEIAHGHVHGPHGHHH
                     "
     gene            8439..>9900
                     /locus_tag="PK144_09630"
     CDS             8439..>9900
                     /locus_tag="PK144_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HNF66028.1"
                     /translation="MNAPIRYTLLATAPHTHRFLSRCTVAEPAADGQCFVLPAWIPGS
                     YLIREFAKNVIAFTACDGDGAPVAVTKTGKDGWRCAPCRGPLSVELEVHALDLSVRTA
                     WLDTTRAYANGTALFPRLPDRADAPCELEVVLPQGETYAGWRVATGLTALATDARGAG
                     LYAAADYDELVDCPIEIGDWQHARFTACGVPHEIVIAGRCTVDLDRLARDLERVCSAQ
                     IRLFGEPAPFERYLFLVMAVGDGYGGLEHRNSTSLLCARDDLPQPGAAELTDGYRTFL
                     GLCSHEYFHAWNVKRIRPAAFTPYDYQRENYTRLLWAFEGITSYYDDLMLRRSGLIDE
                     AAWLQLLGETATRVWRGPGRHRQSVAESSFDAWTKFYRPDADTPNAVVSYYAKGALVA
                     LALDLTLRRLSRGTRSLDDLMRALWQRYGRPGLGVPEDGIESLAAELAGQPLADFFAA
                     CVHGTGDLPLAELLAGHGLVFRLRPAEGATDKGGRAG"
BASE COUNT         1531 a         3579 c         3484 g         1306 t
ORIGIN      
        1 tccttccgtc tgccgcgagc cgccgccatg accgatgccc cgacccctgc cgagctgcgc
       61 gcccggctcg ccggaacccc cgacaccgcc gggctgcccg aggccctgct cgaacgtctg
      121 gccgccgaag acccggcgct gcgcgcgcag gcggccgcgg agctgaccga gctgtacaac
      181 ctgacctggc gcgaaggtca ggcgccggtc gacgaggccg gcttcgcggc ggcgctcacg
      241 ctcgtgcagg tcggctggca ggccggtgcg ctgcagtggc tgcgctacac ggcgccggcg
      301 ctgatcgggc cggcagcggt cgtgctgtac gccgatgacg agggccgggg ctttcgcgcc
      361 tgcatcgagg cctgagcacg gaatgcctgc cggattgagg tttgattgat ccgggtccgc
      421 agcacttccg atgaacagac cgtaagattc gcgcccgcgg ccggcctctg ctccggccgc
      481 gtccgtttcc cctgtccgct ccgcccggcc cgtacaccag aaccagaagg ctcatgccca
      541 tgccgtcttc cgttgcgctg ctcccgatcc gtgcccgctc cctgctgctc gccgtcgcgc
      601 tcggcgcggc cgccctgccg gcagcggccg gcaagtacac cgatcaggtc aacatcctgc
      661 tcgatcgcgt cgataccgcg atggcgaagc gcggcttcga gcagacgcac acgccgaaga
      721 tcgacaagct gcgacagggc gacagcgaca gcttctcgtt cacgctgaag aagggcatgt
      781 cctacaagat cgtgtcggtc tgcgatgccg actgcagcga tctcgacctc gtgctgcggg
      841 acgagggcgg caacgacatc gacaacgacg accagcgcga cgacatgccg atcgtcagcg
      901 tccagccgaa gtggaccggc aagttcacgc tgcgcgtgaa gatgtactcg tgcaacaaca
      961 acccctgcta ctacggcatc ggcatctacg gcacacgctg atgcgccagg gccggcggcg
     1021 ccggacgccg ccggcggttc gggcgcggcg ccgtcaccag cgccggtagc ggtacggatg
     1081 ccaccagccg ccgaccacca ccggcggcgc gacgacggcg accggcgccg gccggtagcc
     1141 ataaccgtat ccgtagccgt atggctcggc cggcaccacg gcgcaggccg atacgccggc
     1201 ggcgagcgcg agacagagcc cgcaacgggc aacggcgttc acgcccagtg tcccggcacc
     1261 cacacccagc cgcgcgggcg ctgatcccag tgacccggca cccagccggc gccgaggcgc
     1321 ggcggctgca gccagtggcc gttctgccag acccactcgt tgccgacccg ctgccagtgg
     1381 ccggcgaccc agacgtggcg cggcgacggg cgctcgatga tgacttcctc gcgcggcggc
     1441 ggcggcgctt cggtgacgat gacttcctcg accacgcgcc gtaccggcac gaactccccg
     1501 gccacggccg cggtgagcgg tgccgcgacg gcagcggcga gcagcagggc tgcccggatt
     1561 cgacgggatg ccatgatggc ggtcctcccg aggacggatg acaccggaaa cgtccggcac
     1621 gcccgcttcg agccggcgcg ccgcgccgcg ttcgcgccgg atcgcttagg cgaggcttaa
     1681 gccggggcgt cgccggtggc gagccggcgc agccagaaac cggcgatcag cgccgctacc
     1741 gtcagtccga ccgcgctgcc ggccagcacg tcgctggccc agtgcttggc gagcaggatg
     1801 cgcgtcagcc cgatcagcgc cgcgaagccg agcacccagg cgcgctgcca cgggcgcgcc
     1861 agcagcaggc agaggcagaa ggcgctggtg gcgatgtcgg ccgagtgccc ggacgggaag
     1921 ctgttgtagg cggcattcca ggtcagcccc agccactgca gcggcgcatc gccggcgccg
     1981 gtgtcggggc gcgggtgtgc gatccacatc ttcagcacgc gcacgacgag caggccgccg
     2041 atgagttgcg cggcgagcca tacgcgcgcg acttcgaacc acagccgctg acgccgccac
     2101 cagccgagca cgagcagggc gatgaagcac gcatagccgg cgtacaggcc ggcgtcggtc
     2161 agccacttca gcaggctgcg ctgtccggcg acccagaagc ggctggagag gtgccggctg
     2221 agcggcgcat cgccgatgag tgccgtcagc agcgccgcca gcgcgagcag cgcgaaggcg
     2281 agcgcgagcg tggcgtgggc gcgggcgcgc acgttcgcga acatcgtcgc cgtcagcggc
     2341 tgcccggcga agcgctgcgg ctgcgcgcgc agcagcgcgc gagcgatgcc ctcgtcagcg
     2401 cgggcagccg gcaaggacac ggtattcctt cagctcgacc gagtaggtct cgtcggtcgt
     2461 gcaggccggc accttgcctt ccgggaacca gtacgacagg tgcgcggact ggatcacgac
     2521 cagcgcatcg ggatgcagca gataccacgc ggcgatgtcg gtgcgctcga cctcggtcat
     2581 cagccggccg gcgtagtagt tcaggatgcc gcgcgtcgaa tgcacgatcg ccaccggccg
     2641 cgagccgggc gccagccggg cgatggcctc gccgagcggc ttgccggagt acagcgggtt
     2701 gacgagcgtg atcagcgcgc cgaacaccac ccaggacacc acggccgaca gtccggtcca
     2761 gccgatcagc cagcgtcgcg gcggctggcg cgacagccgg aagccgagga tcgcgcacag
     2821 gccgaggatc gtggccagcg cgaagccgcg cgggttgttg ccgagcacgt cgtgcgtgcc
     2881 ccagccgatg ccgccggcga gcagcgtgaa ccacaccacc ggcagcagcg cctgttcggt
     2941 gccgatgcgt gactgcgcga gcagcgtcgt gcagccgacg ccggcgagca ccgcgcagag
     3001 cggcaacagc ggctgcaggt agatgaacag cttcccggag atcgccgaga tcgcgagcag
     3061 cgcgaccagc aggcaggcga ggacgaagcg cgccgcgatc ggttcgctct gccagaacgt
     3121 gcgcgcgccg ctgaccgcca gcccggtcca cggcatcagc agcaccggca gcagcgtcac
     3181 gtagaaccag accggctccc ggtgcgacca ggaattcacg gcccggccga ccagctgctc
     3241 gttccagatg gtcacgagat acgcgctctc gccgtcgagc cagaccgccg cgatccacgc
     3301 cagcacgccg ccgaccagca ggccgaggcc gtgcagcggc ttcagcgcgg cgaagcccgc
     3361 ccagtcgcgc gcgccgaggg ggcgcagcag cgccggcagc accgtgaaca gcagcgcgat
     3421 cggacccttg atcgcgacgc cggcggcact cgcgagccag aagccgacga gcgccgcgcg
     3481 cgagcggcgg gtctcatgcg cgtgcagcgc cacccaggcc cagacgcagg tcgcgcagag
     3541 cgtcatgtcc atgcgcgcga tctggccgac caccagcgtg ccgaacagcg tcacgaagca
     3601 gagtgctgcc cagcgcccgg ccgccggacc ggcgaggcgt cgcccgagca cccaggtgcc
     3661 gaaggtcgcg gcgacggtcg acagcagcgt gaacagccgg aacgccagct cgccctggcc
     3721 ggtcagcgcg ccgagtgcgg cgaccagcca gaagtacacc ggcggcttgt ccggatacgg
     3781 gaagccgttc aggtgcggca ccagccagcg cccggattcg agcatctcgc gcagcacctc
     3841 gccgtagcgg gcctcgtcct gcacccagag atcgcgatgc acggcgaggt tcagcgcatg
     3901 cacggcgagc agggcgagca cgagcagcgc ggcatcgcgc cagaaggcat cccggccggc
     3961 agcgggggcg gcgaccggtg acatcattcg gcaggcttcc gggcggcttc gccggcggcc
     4021 cggcagcgtt cgagcagcgc ctcgatcgcc gggcgtccga gcgtgcgcag cttcgcgatg
     4081 ttggcgtccg ggatgccgtc gacatccgga tgcgtggcgg cggcgcgctc gatgtcgtcc
     4141 tcgcggatca ggtgcagcac cggatgcggc gagcggttgg tccagtgcga gacatcgtcg
     4201 ggggcctcgt cggcgaactg gtatccggga tggaacgagg ccacctgcag cacgccggcg
     4261 agcccggctt cctcgaccag cgcatcggcg acatcgagga agtcgttgaa gtccaggaag
     4321 tccgcgaagg cgttcggcag gatcagcagc gtggtctgca cgatcgccgg atcggcctcg
     4381 gcgagcagcg cgagttcgcc ggccagccgt tcggcgatga ccgccggctg cgaggcgtcg
     4441 cagaccgcga agcggatcgt cttcttcgtg aacggcagcg acgcgaacgg gcagagattc
     4501 agtccgacga cgaggtgttc gacccaggcg cgcgtggcgg cgagggccga ttcggattcg
     4561 atggctgcgg gcatggacgc gaccgggtcg ggggctgccg gcaccggccg gcggcagaag
     4621 ccgggtattg tgccgatccg cgccggtccg tccagccgca cagcccgcgg ccgccggcgc
     4681 cgaggccggc gacagccgtc gcgctgcagc accggggcag gttggacccc cgtgcgcccg
     4741 gtgctactct ggccgggcgc aggccccgct gcggcggggg cgacgaaacg acagagactc
     4801 tggcaaaacc tgggggatac atcatgaaga tcaaggacct gctggcgacc aagaccggtc
     4861 gtcccgtgac ggtggcgccg acggcgacca tcgctgaagc cgtcagggcc atgcacggca
     4921 accgcgtcgg cgcggtgctg atccccgacg ccaccggctg tccgctcggc atcttcaccg
     4981 agcgcgacgt cgtgcgcctg tacgccgagg gcgatgacct gacgcaggtg accgtcgagc
     5041 agcgcatgac ctgctcggtg gtgctcggca aacccgatga cagcatcgac tcggtgatgt
     5101 cgctgatgac cgagcggcgc ttccggcatc tgccggtcgt cgacggcggc acgctgctcg
     5161 ggctggtctc gatcggcgac gtggtcaagg ccaagctcga cgaggccgcc aaggaagccg
     5221 agacgctgcg cgagtacatc cactcctgag ccctgccggc cggaccggcg cgtgccgccg
     5281 gtccggccgc ttcacccggt ttcgccgatg ccgccgttct ggcagaccaa gtccctcgcc
     5341 gagctgagcc cgcaggaatg ggaatcgctg tgtgacggct gcgggcgctg ctgcctgcac
     5401 aagctcgagg acgaggacac cggcgagctg ttccacaccc gcgtcgcctg ccgcctgctc
     5461 gatacccaca gctgccgctg cacgcgatat gcgaaccgcc ggcgctgggt gccggactgc
     5521 gtgcggctca cgcccgccga cgtgccgcgc tttcgctggc tgcccgagac ctgcgcctat
     5581 cgccggctcg ccgagggccg gccgctgcct gagtggcatc cgctgctgac cggggatcgc
     5641 gaatccgtgc atgcggccgc cgcctccgcg cgcggctggt gcatcagcga gcgcgacggc
     5701 gtcgacctgc acgcgcacat catcggacgc tagcgtccgg cgtggttccc cgaaaaccac
     5761 cgaaggccac cgaaagccat tgaaaatccc tcgacccccc cgcgaccgat cccgcccatg
     5821 aacctgcgct gctttaccgt cggcctgttc caggtcaacg cctggctgat cgaagacccc
     5881 gagaccgggg cctgtgtcct gatcgatacc ggcgagagcg acgaggtcgc cagcgagctg
     5941 gccgcgctca cgccgcggcc ggatctgcgc gcgatcctgc tgacgcacgc gcatttcgat
     6001 cacgccggcg gactcgtgcc gctgcaggcg ctgtatccgc aggccgagac ctgcctgccg
     6061 cggctggagc gcccgatgtt cgacctgctg ccgcgtcagg gcgagctgtt cggtgccccg
     6121 cagttctcga agccctgcgg gcgcatcgac cgcgagatcg acgatgacga actcgtgcgg
     6181 gtcggcgcgc tcgaatgccg cttcgtgtcg acgcccggac acacgccggg gcagggctgc
     6241 tggcaggtcg gcgggcacct gttcgtcggc gacacgatct tcgccggctc ggtcgggcgc
     6301 accgactttc cgctcagcga tccggggctc gcgcagaaga gcctcgcgaa gctgatgacg
     6361 ctgcccggcg agctgcacgt gcactgcggc cacggcccgg acacgacgct ggccgccgag
     6421 ctgaagggca atccctacct cgcgcacgtg cgccgtgcgc tcggcatcaa ggagccgcgc
     6481 ggcggctacg cccgcggcgg caactggggc tgagccgcga tgtgcgagct gctcggcatg
     6541 agcgcgaacg tgccgaccga catccgcttc agcttcaccg ggctgatgca gcgcggcggt
     6601 cacaccggtc cgcaccgcga cggctggggc atcgcattct acggcgagcg cggcgcgcgg
     6661 ctgttccacg acccctgccc gagctgcgaa tccgaagtcg cgcgcctcgt gcagcagcat
     6721 tcgatcaaga gccgcatcgt catcagccac atccgccgcg ccaaccgcgg ccgggtctgc
     6781 ctggagaata cgcatccgtt cacgcgcgag ctgtgggggc gcagctgggt gttcgcgcac
     6841 aacggcgagc tgaagggcat caagcgccgg ccctgcggcc cgtatgagcc gatcggcagc
     6901 accgacagcg agcatgcgtt ctgctggctg ctcgggcgca tccgcgagcg cttcccgcag
     6961 ccgccgcgcg tgccggcgac gctgtggcgc tacgtcgccg ggctcgcggc cgagctgaac
     7021 cagctcggca tcttcaactt cctgctctcc gacagccgct ggctgtacgc gcactgcagc
     7081 acgaagcttg cgtggctgac gcggcgcgcg ccgttcggcg aggcgcgcct gaccgatgcc
     7141 gacatggccg tcgacttcca gggcgagacc acgcccgatg acgtggtcac cgtcatcgcc
     7201 acgcagccgc tgaccggcaa cgagcgctgg aacctgctcg cgcccgggga tttcatcgtg
     7261 ttcgacggcg gcgaaccggt ctggcagcac ggggtgccgg ccggctgcgc gacgccgtcg
     7321 gcgttcctgc atcccggctg acgaagcgct cgcgcacggc cgctcagccg tcgagatagc
     7381 ggacgcagcc gatcaggttg cgccgccagt acggcgtgtc gaggctcgac ttcagcaccc
     7441 ggccgccgcg gctcgatgcg tggatcatgc ggccgtcacc ggcgtagatg ccgacgtgcg
     7501 tcggcttgcc gggttcgttc tcggagaaca gcagcaggtc gccgggctcg gggtcggcgg
     7561 ccggtcggcc gtcgcgcagc agcgcggtgg tggtgcgcgg cacgcgcagg ccggcttcgg
     7621 cgtaactgag ctgcacgagg ccgctgcagt cgaggccgct gcgatcggtg ccgccccagc
     7681 ggtagggcgt gccgatcagc gcctcggcac tggcgagcac gcgcgcgcgc cgttcggaca
     7741 gccccgatgc gcagccggcc agtacgacgg acaggcagac gtacgcgagg cggcggagca
     7801 tggcggcggc accggaaggc ggcagggcag gggatgcgcc cgagtatagc cggcgggcgc
     7861 ctgtgctaag gtcgtgcctt cccgtcccct cccagcggta aaccctccat gaaagcagct
     7921 tccggcatgg tcgtctccat gcattacacc ctgaccgacg acgccggcga agtgctcgac
     7981 accagccagg gccgcggtcc cttcgactac ctgcacggcc acggcaacat catccccggt
     8041 ctcgaaagcc ggctcgaagg tgccgagccc ggcttcaagg acaaggtcac ggtcgcaccg
     8101 gccgacggct acggcgaacg caacgaccag gccgtgttcg acgtgccgcg cgaccagttc
     8161 ccgccggacg aggagatccg cgtcggcatg caggtgcacg gcgaaggccc gcagggcgtc
     8221 atcgccttca ccgtcgtcga tgtcgccgag gacagcatcc gcctcgacgg caaccacccg
     8281 ctggccggca agacgctgca tttcgatgtc gagatcctcg acatccgcgc cgcgtccgag
     8341 gaagaaatcg cccacggcca cgtccacggc ccgcacggcc accaccactg agccgctgcg
     8401 accgcggggc cggcccccgg tcgcttcccg atgcctgcat gaacgcaccg atccgctaca
     8461 cgctgctcgc cacggcgccg cacacccacc gcttcctcag ccgctgcacg gtcgccgagc
     8521 cggctgccga cgggcagtgc ttcgtgctgc cggcgtggat tccgggcagt tacctgatcc
     8581 gcgagttcgc gaagaacgtc atcgcgttca cggcgtgcga cggcgacggt gcgccggtgg
     8641 cggtcacgaa gaccggcaag gacggctggc gctgcgcgcc gtgccgcgga ccgctgagcg
     8701 tcgaactcga agtccatgcg ctggatctct ccgtgcgcac ggcctggctc gacaccacgc
     8761 gcgcctacgc caacggcacg gcgctgttcc cgcggctgcc ggatcgcgcc gatgcgccct
     8821 gcgagctgga agtcgtgctg ccgcagggcg agacctacgc cggctggcgc gtcgcgaccg
     8881 ggctcacggc gctcgccacc gacgcgcgcg gtgccggcct gtacgcggcg gccgattacg
     8941 acgagctggt cgactgcccg atcgagatcg gtgactggca gcacgcgcgc ttcacggcct
     9001 gcggagtgcc gcacgagatc gtcatcgccg ggcgctgcac ggtcgatctc gaccggctcg
     9061 cgcgcgacct ggagcgcgtc tgcagcgcgc agatccggct gttcggcgag ccggcgccgt
     9121 tcgagcgcta tctgtttctg gtcatggcgg tcggcgacgg ctacggcggg ctcgaacacc
     9181 gcaactccac gagcctgctg tgcgcgcgcg acgacctgcc gcagccgggg gctgccgagc
     9241 tcaccgacgg ctaccggacc tttctcggtc tctgcagcca cgagtacttc cacgcctgga
     9301 acgtcaagcg catccggccg gcggcgttca cgccgtacga ctaccagcgc gagaactaca
     9361 cgcggctgct gtgggccttc gagggcatca cctcgtatta cgacgacctg atgctcaggc
     9421 gctccggcct gatcgacgag gccgcctggc tgcagctgct cggcgagacc gcgacccgcg
     9481 tctggcgcgg gccggggcgg catcgccagt cggtggcgga gtcgagcttc gatgcgtgga
     9541 ccaagttcta ccgccccgac gccgataccc cgaacgccgt ggtcagctat tacgccaagg
     9601 gcgcgctggt ggcgctcgcc ctcgacctga cactgcgccg cctgagccgg ggcacgcgct
     9661 cgctcgatga cctgatgcgc gcgctgtggc agcgctacgg ccggcccggc ctcggcgtgc
     9721 ccgaggacgg catcgaatcg ctggccgccg agcttgccgg ccagccgctc gccgacttct
     9781 tcgccgcctg cgtgcatggc accggcgatc tgccgctcgc ggagctgctc gccggacacg
     9841 gcctcgtgtt ccggctgcgt ccggccgagg gcgccaccga caagggcggc cgggccggca
//
LOCUS       DAOQIE010000132         9726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3550_length_9726_cov_7.350222, whole genome shotgun sequence.
ACCESSION   DAOQIE010000132 DAOQIE010000000
VERSION     DAOQIE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_9726_cov_7.350222"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..622
                     /locus_tag="PK144_09635"
     CDS             71..622
                     /locus_tag="PK144_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66029.1"
                     /translation="MPSSSRWLRRLLLSPLVLLAALVLLFEEWGWRPLARAMAALGRL
                     PWLRALEARIARLSPWGAAVAFIVPAAVLLPFKLAALALIAHGALVPGVFVILLAKLT
                     GTAIAARLYVLCEPQLMSLGWFAWARAKLLAFHDLVHAWLARQWLWQATHALVLAVRR
                     RVQALKPGVLGRWWRRRRSAGRG"
     gene            782..1423
                     /gene="lexA"
                     /locus_tag="PK144_09640"
     CDS             782..1423
                     /gene="lexA"
                     /locus_tag="PK144_09640"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNF66030.1"
                     /translation="MSALTLTEQRIYRYIRDFMEETGRGPTRAEIAAGVGMKSRGTVQ
                     RYVQALAAAGLIEIDGQRWHGIRLKSAQVPVDARADAHPDLTGAEDWSLPLLGRIAAG
                     QPIEAVADEQTLDLRLLGGANRYALRVRGESMIEAGILDGDTVIVEACEAARNGDIVV
                     ALIEGGEVTLKYYHPRPDGTVLLVPANARMEPMRFRADSVRVQGRVVGQLRLY"
     gene            1603..1746
                     /locus_tag="PK144_09645"
     CDS             1603..1746
                     /locus_tag="PK144_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66031.1"
                     /translation="MCPSPFVAVSHDSGLSAGHPMRRWVLRAWRFGLIGNRRAARMLS
                     RLA"
     gene            complement(1943..2545)
                     /locus_tag="PK144_09650"
     CDS             complement(1943..2545)
                     /locus_tag="PK144_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF66032.1"
                     /translation="MSTKPVIGLLLAAGLSSRYRAVSGRHKLLEAVPDGLPGAGTPLA
                     LCAARSLLAGADAVLAIVRPEDEPLAGLLAAAGCEVLDFASPGLGASLAFGVRARPEA
                     GGWLVLPADLPFVRPSTVQAVRAALAVSPCAAPAYRNQRGHPVGFGARHYSALAALDG
                     DRGARDLLETRGWQRIGVDDPGCVADIDVPADLEACLRPR"
     gene            complement(2542..3450)
                     /locus_tag="PK144_09655"
     CDS             complement(2542..3450)
                     /locus_tag="PK144_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF66033.1"
                     /translation="MDSLTGLRVFVKVVETGSFTAAAEALELSRAMVSKHVQTLEARL
                     GVRLLQRSTRRVALTEAGSRYHARASAWLEDMDTTEAELGQLTTEPRGTLRISAPMSF
                     GRVYVAPLLGEFLNRHPGLRIDLALADRAVDLIEDGYDLAVRIGELADSSLVCRRLAA
                     TRLLLCASPGYLARYGTPDHPAELSGHECLSYRLSDTPSDWHFSDADGHTATVRIDGR
                     VRADNGEALCEAAANGLGIVLQPDFIAGPFLQAGRLLALLPDWNTREIGIHAVYPERR
                     YLPARARRFIDFLAERYALAPWRMES"
     gene            complement(3619..4233)
                     /locus_tag="PK144_09660"
     CDS             complement(3619..4233)
                     /locus_tag="PK144_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNF66034.1"
                     /translation="MEPSHSAALLSLFPDGMPAARMWPSRPEQNIKQLYLNGFNQHGT
                     DTGLLPLPGRKGRRGMFPDIATEVSVMRNNKNSSLIAATLTLALGAGAAWAANPVNPG
                     DAPTTTPSGRTPSGQVMPGGSEGAKTDATVVPQPVDKTMFPTFAQADLNKSGTIEPTE
                     IGMVKGLDFQKADVDHNGTLSKTEYEQAVQSATAPAGKLKDSRG"
     gene            complement(4400..5152)
                     /locus_tag="PK144_09665"
     CDS             complement(4400..5152)
                     /locus_tag="PK144_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HNF66035.1"
                     /translation="MTLTAAGERLLERARALLREAGAAAEEVRRIGRGELGELRIAFT
                     SSLPFSELIPRLLYDYRRRYPDVRLELRDTGTQRQLDLLLAGRLDLGFVRTDDPQLPA
                     GLAWTELHRDPLRLVIHEAHPLAGRSEVAIAELAEEAFVMFPEDSGTGLLRQFHALCR
                     TAGFTPHIVQEAREVSTIIGLVAAGLGLSILPAPLACIGIARVRFLALTDAAAQSIVL
                     LATRAGPLPAAVANFVEQAATGARLGAPPTTG"
     gene            complement(5337..5462)
                     /locus_tag="PK144_09670"
                     /pseudo
     CDS             complement(5337..5462)
                     /locus_tag="PK144_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            5604..6869
                     /locus_tag="PK144_09675"
     CDS             5604..6869
                     /locus_tag="PK144_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66036.1"
                     /translation="MSAPALAASRLPAMHHAGFTDPAGPQAGIRVGTPEYRQMRRALF
                     LGGFATFSLLYCVQPLLPLYSHEWQVAPAQASTVLSAATGGLALTLILASAVSDRLGR
                     RGLMAGAVLLASLLGLACSMAAGLAQLVWLRALEGIVLAGLPAVAMAYVSEEMEPRGL
                     ATAMGLYIAGSAFGGMSGRIATAWLAELWDWRSAMAAQALLSLGIGLVFWKSLPASRA
                     FRPRPLRLDALRAGLRLHLSDAGLPWLYALAFLLMGCFVSLYNYLGYRLAGAPFGFGA
                     GWIGTLSVFYVSGMLGSGWTGRLTARYGVQRVIMVMTLLMLAGLLATLSEALPLLLAG
                     VALATFGYFGAHAGASAWVGRRAREARGLASALYLSAYYAGSSLLGSVSGLVWHLDGW
                     SAVVALLATLLAGCLLIAWRLRRLAASEA"
     gene            complement(6936..7568)
                     /gene="ribA"
                     /locus_tag="PK144_09680"
     CDS             complement(6936..7568)
                     /gene="ribA"
                     /locus_tag="PK144_09680"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNF66037.1"
                     /translation="MPAQPHPPLPPALVRRKVTVPLADGTPALFHSFDGLVDGREHLA
                     LGLGPQPADGIPLVRLHSECLTGDVFGSQRCDCGPQLEEALQRIGTAGGYVLYLRQEG
                     RGIGLYAKLDAYALQDRGLDTYAANRHLHQPEDARDYRCAAQMLVALGVTRIRLLSNN
                     PDKAEQLAQHGIEVIERVPTGVFLTAHNHAYLSAKVRFGSHDLDIDDTAP"
     gene            complement(7780..7989)
                     /locus_tag="PK144_09685"
     CDS             complement(7780..7989)
                     /locus_tag="PK144_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNF66038.1"
                     /translation="MSEHVYKKIELVGSSPVSIEAAIENAVARAAQTLHSMRWLEVVE
                     TRGHIENGKVAHWQVTIRIGFTLDG"
     gene            complement(8211..9320)
                     /locus_tag="PK144_09690"
     CDS             complement(8211..9320)
                     /locus_tag="PK144_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNF66039.1"
                     /translation="MKTPANSAAALLSPYSLGPLTLRNRVVMAPLTRSRADAQTLAPR
                     ALNAEYYAQRAGAGLIISEGSQVSQQGQGYAWTPGIYSEAQIAGWRQVTDAVHALGGT
                     IFIQLWHVGRISHPELQPDGALPVAPSAIRPEGQAFTNDGFKPMVEPRALETSEIPGI
                     VEQFRQAARNARAAGFDGAEIHSANGYLLEQFLRSHTNRRSDAWGGSLENRTRLLLEV
                     VAAVGSVFGLERTGVRLSPATTVNDIGPGEPELAAYVLKALDASGVGFVHMVEGVTGG
                     ARTVEGFDFRALRKLFPRTWIANNGYDGALGAEAVASGHAELVAFGKPWISNPDLVER
                     LATGLPLAQPDKATLYGGDAHGYTDYPRATPAAIA"
     gene            9502..>9726
                     /locus_tag="PK144_09695"
     CDS             9502..>9726
                     /locus_tag="PK144_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF66040.1"
                     /translation="MSALHLYAVLVEAGSFTRAADRLGCTKGHVSKQLAALERSLGMQ
                     LLHRSTRRLAPTAAGSALLPLARRLTAVDDE"
BASE COUNT         1340 a         3330 c         3556 g         1500 t
ORIGIN      
        1 ggcggcgcgc tggtcgatgc gcatgccgcc ccgatgtccg cagttcaccc gaacgccgag
       61 attcccgccg atgccctcgt catcccgctg gctgcgccgt ctgctgctgt ccccgctggt
      121 gctgctcgcc gccctcgtgc tgctgttcga ggaatggggc tggcggccgc tcgcgcgagc
      181 gatggccgcg ctcgggcgcc tgccgtggct gcgggcgctg gaggcgcgca tcgcgcggct
      241 gtcgccgtgg ggcgcggccg tggccttcat cgtgccggcg gcggtgctgc tgccgttcaa
      301 gctcgcggcg ctggcactga tcgcgcacgg tgcgctcgtg cccggcgtgt tcgtgatcct
      361 gctcgcgaag ctgaccggca cggcgatcgc cgcgcggctg tacgtgctgt gcgagccgca
      421 gctgatgagc ctcggctggt tcgcgtgggc ccgcgcgaag ctgctcgcct ttcatgatct
      481 cgtgcatgcc tggctcgcac gccagtggct gtggcaggcg acgcatgcgc tggtgctcgc
      541 cgtgcgtcgg cgcgtacagg cactgaagcc gggcgtgctc ggccggtggt ggcgccggcg
      601 gcgcagcgcc gggcgcggct gaccgggccc ggccggctgc ggagcaaagg aggtagcgca
      661 cgacccatac ggatgcatcg ctgctctgcg cgcttgcagg catgtgccgt ccggacagcg
      721 cttgcttgta ctccaaatgt tctctattct tcgctccatg gtcgctcatg gagccgaacc
      781 gatgtctgcg ctgacgctga ccgaacaacg catctaccgc tacatccgcg atttcatgga
      841 agaaaccggc cgcggcccga cgcgcgccga gatcgccgcc ggcgtcggca tgaagtcccg
      901 cggcaccgta cagcgttatg tacaggcgct cgccgccgcc ggactgatcg agatcgacgg
      961 gcagcgctgg cacggcatac ggttaaagtc cgctcaggtt ccggtcgatg cccgtgccga
     1021 tgcccatccg gatctcaccg gggctgagga ctggtcgctg ccgctgctcg gacgcatcgc
     1081 cgccggccag ccgatcgagg cggtcgcgga cgaacagacg ctggacctgc gcctgctcgg
     1141 cggtgccaac cgctacgcgc tgcgcgtgcg cggtgaatcg atgatcgagg ccggcatcct
     1201 cgacggcgac acggtgatcg tcgaagcctg cgaagcggcg cgtaacggcg acatcgtcgt
     1261 cgcgctgatc gaaggcggcg aggtgacgct gaagtactac cacccgcgcc cggacggcac
     1321 ggtgctgctg gtgccggcca atgcccgcat ggagccgatg cgctttcggg ctgactcggt
     1381 acgggtgcag ggacgcgtcg tgggtcagtt aaggctgtat taacgccggt ctgacaccgg
     1441 caacccggat cgtgatttcc ggtcgcatcg ttgtcagatg acttgcatgg aatgggggga
     1501 acgatgcgta aatgtccgtc tgccggctgc gttcccccgc gaccggcatc cattcacggg
     1561 gcccaagagg tcaccggtcc gatcaagaca ggagcagcaa acatgtgccc gagcccgttc
     1621 gtcgccgtat cccatgattc cggtctctcc gccgggcacc cgatgcgtcg ctgggtcctg
     1681 cgcgcctggc gcttcggcct gatcggcaac cgccgcgccg cacgcatgct gtcgcgcctc
     1741 gcgtgaggcc gcgggcgcag ccgaaggtgg attcgccggc gtgcgggcgc cgggtttcca
     1801 cgagcggctg acgcaggccc gggcggtgtc gctcacggac gccgcaccgc gttcaccgct
     1861 tgttgcaccg tctgttccag ggcatcgccc ttccggtttc cggaccgccc ggtccgctgc
     1921 ctgaaagccc cgcgaagcgg catcagcgtg ggcgcagaca ggcttccaga tccgccggca
     1981 cgtcgatgtc ggccacacag cccggatcat cgaccccgat ccgctgccag ccccgggttt
     2041 ccagcaagtc gcgcgcaccg cgatcgccat cgagcgccgc gagcgccgag tagtgtcgtg
     2101 cgccgaagcc gaccggatgg ccgcgttggt tccggtatgc aggggcagcg caggggctga
     2161 ctgccagtgc ggcgcgcacg gcctgcacgg tcgaaggccg cacgaagggc aggtcggccg
     2221 gcagcaccag ccagccgccg gcctccgggc gggcacgcac tccgaacgcg agactcgcgc
     2281 cgaggcccgg acttgcgaag tccaggactt cgcagccggc cgcggcgagc aggccggcga
     2341 gtggttcatc ctccggtcgc acgatcgcca gcaccgcgtc cgcaccggcc agcagcgagc
     2401 gcgctgcaca cagcgccaga ggcgtgccgg cgcccggcag gccatccggc accgcttcga
     2461 gcagcttgtg ccgaccgctg acggcgcggt aacggctgct gaggccggcg gcgagcagca
     2521 ggccgatgac cggtttcgtg ctcatgactc catccgccac ggcgccagcg cgtagcgctc
     2581 ggccagaaaa tcgatgaagc gccgggcccg ggccggcaga tagcggcgtt ccgggtaaac
     2641 ggcgtggatg ccgatctcgc gcgtgttcca gtccggcagc agcgcaagca gccggccggc
     2701 ctgcaggaaa gggccggcga tgaagtccgg ctgcagcacg atgccgagcc cgttcgcggc
     2761 cgcctcgcac agcgcctcgc cattgtcggc acgcacgcgg ccgtcgatgc gcacggtggc
     2821 cgtgtggccg tcggcgtcgc tgaagtgcca gtccgaaggc gtgtcggaga gccggtagct
     2881 caggcattcg tgaccggaca gctcggccgg atggtccggc gtgccgtagc gggcgagata
     2941 gccgggcgag gcgcacagca gcaggcgggt ggcggcgagg cgccggcaga cgaggctgga
     3001 gtcggcgagt tcgccgatgc gcacggcgag gtcgtagccg tcctcgatca ggtcgacggc
     3061 gcgatcggcg agcgccaggt cgatgcgcag gcccgggtga cggttgagga actccccgag
     3121 cagcggcgcg acgtagacgc gcccgaagct catcggtgcg ctgatgcgca gtgtcccgcg
     3181 cggctcggtg gtgagctgtc caagctccgc ctcggtcgtg tccatgtcct ccagccaggc
     3241 gctggcgcgc gcgtggtagc ggctgccggc ttcggtcagc gccacgcgcc gggtcgagcg
     3301 ctgcagcagg cgcacgccga ggcgtgcctc cagcgtctgc acgtgcttgc tgaccatcgc
     3361 ccgcgacagc tccagcgcct cggccgcggc cgtgaagctg ccggtctcga cgaccttgac
     3421 gaagacccga aggccggtga gggagtccat gatcgggaag gccccccggc gtccgggccg
     3481 aagccgccgg agccgtcttg ctgcgcgaag gaccgacaag tgtacggccc gctcccccgc
     3541 aggaggaacg ggccgtgacg aggggccgcc gctgccggga aaccccggca gtggcgtatc
     3601 gcgcgaccgg gggccgtgtc agccgcgcga atccttgagc ttgccggccg gcgcggtggc
     3661 cgactggacc gcctgttcgt actcggtctt gctcagcgtg ccgttgtgat cgacgtcggc
     3721 cttctggaaa tcgagaccct tgaccatgcc gatctcggtc ggctcgatgg tgccggactt
     3781 gttcaggtcg gcctgggcaa aggtcgggaa catcgtcttg tcgacgggct gcggcacgac
     3841 ggtcgcatcg gtcttcgcac cttcggaacc gcccggcatc acctggccgg aaggcgtacg
     3901 gccggacggc gtcgtggtcg gcgcatcacc ggggttgacc ggattggccg cccaggcggc
     3961 accggcgccg agcgcgaggg tcagggtcgc agcgatcagg gacgagttct tgttgttgcg
     4021 catgacggaa acctctgtgg cgatatcggg gaacattccc cgccggcctt tccggcctgg
     4081 cagcggcagt agtccagtat ccgtgccatg ttgattaaat ccgtttaaat acaattgctt
     4141 gatgttttgt tccgggcgag acggccacat gcgggcagcc ggcatcccgt cgggaaacag
     4201 cgacagcagt gcggcggagt gagatggctc cacgaaaaat caatgacttg caaagtcgcc
     4261 gtgcagacac gccgctttcg gcgcagcgcg cctcagagga aggaattatc cagacaatct
     4321 gtggataact gccggccgga cggcaagcgg accgcggagg tactgcttgc ggcagcgatg
     4381 gcgaaattcc cgccagtcgt caaccggtgg ttggtggtgc cccgagccgc gcaccggtcg
     4441 ccgcctgctc gacgaagttg gcgaccgcgg ccggcagcgg accggcgcgg gtggcgagca
     4501 ggacgatcga ctgcgccgcc gcatcggtga gcgccagaaa gcgcacgcgc gcgatgccga
     4561 tgcaggcgag cggcgccggc aggatcgaca ggcccagccc ggcggcgacc aggccgatga
     4621 tcgtcgacac ctcgcgcgcc tcctgcacga tgtgcggcgt gaagccggcc gtgcggcaga
     4681 gcgcgtggaa ctggcgcagc agcccggtcc cggaatcctc cgggaacatc acgaacgcct
     4741 cctcggccag ttcggcgatc gccacctcgc tgcgtccggc gagcggatgc gcttcgtgga
     4801 tcaccagccg cagcggatcg cggtgcagtt cggtccaggc gagcccggcc ggcagctgcg
     4861 gatcgtcggt gcgcacgaag ccgagatcga ggcggccggc gagcagcagg tcgagctggc
     4921 gctgcgtgcc ggtgtcgcgc agttccagcc gcacgtccgg atagcggcgg cggtagtcat
     4981 acagcaggcg cggaatcagc tccgagaacg gcagcgagct cgtgaaggcg atgcgcagct
     5041 cgccgagttc gccgcggccg atgcgccgga cctcctcggc ggccgccccg gcctcgcgca
     5101 gcagcgcgcg cgcccgctcc agcagacgct cgccggcggc ggtgagcgtc acgtgccggc
     5161 gcgagcgctc gaacagcgcg acgccgagtt cggcttcgag cgcctgaatc tgctggctca
     5221 gcggcgagga ttcttttgtg cgcgcacaat gggcggcgcg cccgctccag cagacgctcg
     5281 ccggcggcgg tgagcgtcac gtgccggcgc gagcgctcga acagcgcgac gccgagttcg
     5341 gcttcgagcg cctggatctg ctggctcagc ggcggctgcg cgatgtgcag gcgttcggcg
     5401 gcgcgcgtga agtggcgctc ctccgcgacg gcgacgaaat agcgcagacg gcgcaggtcc
     5461 atgacggcgg ctccgggcaa ggacggtacg gggggcggga ctgatatcga aacgatatca
     5521 atactgcctg aaaaccatat tggacctatc agactgcagc gccctagcct tgtgcatccg
     5581 tgatccccgc tcggtgatgt ccgatgtccg cccctgccct tgctgcttcc cgcctgcccg
     5641 ccatgcacca tgccggtttc accgatcctg cagggccgca ggccggcatc cgcgtcggta
     5701 cgcccgaata ccggcagatg cgccgggcgc tgttcctcgg cggcttcgcg accttctcgc
     5761 tgctgtactg cgtgcagccg ctgctgccgc tgtactcgca cgaatggcag gtggcaccgg
     5821 cgcaggcgag cacggtgctg tcggcggcaa ccggcgggct ggccctgacg ctgatccttg
     5881 cgagcgccgt gtccgatcgc ctcggccggc gcgggctgat ggccggcgcc gtgctgctcg
     5941 cgtcgctgct cggactggcc tgctcgatgg ccgcgggact ggcgcagctc gtgtggctgc
     6001 gtgcgctcga aggcatcgtg ctcgccggcc tgccggcggt ggcgatggcc tatgtgtccg
     6061 aggaaatgga gccgcgcggg ctggcgacgg cgatgggcct ctacatcgcc ggcagcgcct
     6121 tcggcggcat gagcgggcgc atcgccacgg cctggctcgc ggagctgtgg gactggcgca
     6181 gcgcgatggc cgcgcaggcc ctgctgtcgc tggggatcgg gctggtgttc tggaagagcc
     6241 tgccggcttc gcgggcgttc cggccgcggc cgctgcgcct cgatgcgctg cgcgccgggc
     6301 tgcgcctgca tctgtccgat gccggcctgc cgtggctgta cgcgctcgcc ttcctgctga
     6361 tgggctgttt cgtcagcctg tacaactacc tcggctaccg gctggccggc gccccgttcg
     6421 gcttcggtgc cggctggatc ggcacgctgt cggtgttcta cgtgtccgga atgctcggct
     6481 cgggctggac cgggcggctc acggcgcgct acggcgtgca gcgcgtgatc atggtgatga
     6541 cgctgctgat gctcgccggg ctgctcgcga cgctgtcgga agcgctgccg ctgctgctcg
     6601 ccggcgtcgc cctcgcgacc ttcggctact tcggcgccca cgccggcgcg agcgcctggg
     6661 tcgggcggcg ggcgcgcgag gcgcgcggac tggcctcggc gctgtatctc agcgcgtatt
     6721 acgccggctc cagcctgctc ggcagcgtct cggggctggt ctggcacctc gatggctgga
     6781 gcgccgtcgt cgcgctgctc gcgacgctgc tcgccggctg cctgctgatc gcctggcggc
     6841 tgcgccggct ggcggcgagc gaagcctgaa cgcctcgccc cggcccggcg ggcctcttca
     6901 aaggggctgc gcctgtcgct gcaacgggcg ccggatcacg gcgccgtgtc gtcgatgtcg
     6961 aggtcgtggc tgccgaagcg caccttggcg ctgaggtacg catggttgtg tgcggtcagg
     7021 aacacgccgg tcggcacgcg ctcgatgacc tcgataccgt gctgcgcgag ctgttcggcc
     7081 ttgtccgggt tgttgctgag caggcggatg cgcgtgacgc cgagggcgac gagcatctgc
     7141 gcggcgcagc ggtaatcgcg cgcgtcctcg ggctggtgca ggtggcggtt ggcggcatag
     7201 gtgtcgaggc cgcgatcctg cagcgcgtag gcatcgagct tggcgtacag gccgatgccg
     7261 cggccctcct ggcgcaggta cagcacgtag ccgccggcag tgccgatgcg ctgcagggct
     7321 tcctcaagct gcggaccgca gtcacagcgc tgcgagccga acacgtcgcc ggtcaggcat
     7381 tcggaatgca ggcgcaccag cgggatgccg tcggccggct gcgggccgag gccgagcgcg
     7441 aggtgctcgc gcccgtcgac gaggccgtcg aagctgtgga acagcgcggg cgtgccgtcc
     7501 gcgagcggta ccgtgacctt gcggcgcacg agcgcgggcg gaagcggcgg gtgaggctgg
     7561 gctggcatgt tgcaggtgtc cgtggtgggt cgggctgcac gccggcagcg ctggcgcacg
     7621 cgggtcggca gacctcgatg cgcgccgtgc gccgccggcg tccggaaccc ggccggtgcc
     7681 ggtgcggccg gtgtccgccg cagcgggcgg accgaagccc gccactttcg cagcttgtgc
     7741 gcccggcatc cagcccgtcc cgctctgtgg ctcgcaggct cagccgtcga gcgtgaaacc
     7801 gatgcggatc gtcacctgcc agtgcgcgac cttgccgttc tcgatgtggc cgcgcgtctc
     7861 gacgacttcc agccagcgca tgctgtgcag ggtctgcgcg gcgcgggcaa cggcgttctc
     7921 gatggccgcc tcgatcgata ccggcgacga gccgacgagt tcgatcttct tgtagacgtg
     7981 ctcgctcatg ggatgtgcct gctccttcgg tgcggacccg cgcccggaaa cgtcgcacgc
     8041 cgcgggcggg tccatgccgc ggcgcgctcc gaactccaga ccccggacgc acagaaagca
     8101 atcccgcagc gcggcaatcc gcagcgaccg gtcgccggaa gcacgcggca gcgcgggccc
     8161 ggcggcggcg ggcggcgtcg cgcacgacga gcgcccgcgc atgcccgcgt tcaggcaatc
     8221 gccgccggcg tggcgcgcgg atagtcggtg tagccgtgcg catcgccgcc gtagagggtc
     8281 gccttgtccg gctgcgcgag cggcaggccg gtcgcgaggc gttcgaccag gtcggggttg
     8341 gagatccacg gcttgccgaa ggcgaccagc tcggcatgac cgctggccac ggcttcggca
     8401 ccgagggcac cgtcgtagcc gttgttggcg atccaggtgc gcgggaacag cttgcgcagc
     8461 gcccggaaat cgaagccctc gaccgtgcgc gccccgccgg tgacgccctc gaccatgtgc
     8521 acgaagccga ccccgctggc atcgagcgcc ttcagcacgt acgcggcgag ttccggctcg
     8581 ccggggccga tgtcgttgac cgtggtcgcc ggcgacagcc gcacgccggt gcgctccagc
     8641 ccgaacacgc tgccgaccgc ggcgacgact tcgagcagca ggcgcgtgcg gttctccagg
     8701 ctgccgcccc aggcgtcgct gcgccggttg gtgtggctgc gcaggaactg ctccagcaga
     8761 tagccgttgg cgctgtggat ctcggcgccg tcgaagccgg cggcccgggc gttccgtgcc
     8821 gcctgccgga actgttcgac gatgccgggg atttcggagg tttccagcgc gcgcggctcg
     8881 accatcggct tgaagccatc gttcgtgaac gcctggcctt cggggcggat cgccgacggg
     8941 gccaccggca gcgcgccgtc cggctgcagc tccgggtgcg agatgcgccc gacgtgccag
     9001 agctggatga agatcgtgcc gccgagcgcg tgcacggcgt cggtgacctg ccgccagccg
     9061 gcaatctgcg cctcgctgta gatgccgggg gtccaggcat agccctggcc ctgctgcgag
     9121 acctgcgagc cctcgctgat gatcaggccg gcaccggcgc gctgggcata gtactcggcg
     9181 ttcagcgcgc gcggcgccag cgtctgcgca tcggcgcgcg agcgcgtcag cggggccatg
     9241 accacgcggt tgcgcagggt cagcgggccg agggaatacg ggctcagcag ggcggcggcg
     9301 gaattcgcgg gcgtcttcat gatcaaggcc tcatggggtt gcgaacagga aacgattcgc
     9361 actatacaaa gcctttgtcc gccgataagc cgggattccg gcaacgcatc gtttaccaga
     9421 ggaaacgaac ctttgagtac gacccctgcc cgtgcgctgc cggatcgctc ggtcgccgat
     9481 gccgcggccg ccgcgcacga tctgtcggcg ctgcatctgt acgcggtgct ggtcgaggcc
     9541 ggcagcttca cgcgcgccgc cgaccggctc ggctgcacca agggccacgt cagcaagcag
     9601 ctggcggcgc tggagcgcag cctcggcatg cagctgctgc accgctcgac gcgccggctg
     9661 gcgccgaccg ccgccggcag cgcgctgctg ccgctcgccc ggcgcctgac cgcggtcgat
     9721 gacgag
//
LOCUS       DAOQIE010000133         9663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3580_length_9663_cov_6.907369, whole genome shotgun sequence.
ACCESSION   DAOQIE010000133 DAOQIE010000000
VERSION     DAOQIE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_9663_cov_6.907369"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1431
                     /gene="gabT"
                     /locus_tag="PK144_09700"
     CDS             142..1431
                     /gene="gabT"
                     /locus_tag="PK144_09700"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNF66041.1"
                     /translation="MIRMSTNADLMLRRNAALPRGVGQAFPIFIERARNAEVWDVEGR
                     RYIDFGGGIAVLNVGHNHPRVVAAVQAQAEKLAHTCFMVTAYEDYVTVCERLNALSTI
                     PGAKSFLVSTGAEAVENAVKIARAYTKRPAVIAFGGGFHGRTLLGMGLTGKVAPYKAG
                     FGPFPGGLYHAAYPCPLHGVSIDDALASVEKLFKYDVDPQTVAAIILEPVQGEGGFYI
                     APPAFLQKLRALCDAHGILLIVDEIQTGAGRTGTWLAIEQSGGIVPDLITMAKSIGGG
                     YPVAAVIGRPEVMDAPNPGGLGGTYAGNPVACAAARAVLDVFADEHLLDRARQLGEHL
                     RARLEALKTRFASIAEVRGLGPMVAFELCTDADPARPAAELTKALTALAAERGLILLA
                     CGVYGNVIRILVPLTAEPAIVDEGLDILAGCLETLGA"
     gene            1633..3984
                     /locus_tag="PK144_09705"
     CDS             1633..3984
                     /locus_tag="PK144_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66042.1"
                     /translation="MKSKVASALPQLLLALLVLAIVASLGVAGLRLRERLNAPPPDTP
                     NAAHALASTTERALLRVALTIGRLQTGEAPVALREAHAQLAAVLTEAGASTPAASQLL
                     ALPDAAAAWGEARGLADELAPLVKLPQLDAPLLAGRFVPRLDATAARIGKVADAADAA
                     SRQPDPDRNADLRALIALALAGLAGLLLAGTLAHRQSQLARLREQVAHGIAERTVGQQ
                     QTSERLVTLSHAIEQSPAIVLIADASGCVEYANPRFETVTGYPVSEILGKRVGTLPTD
                     RLDADERAQMWADLSAGKIWQRESESRTRRDDPYWERTQIAPVRDLQGELTHFVVVKE
                     DVSERKQFEAKLLRQARYDELTGLPNRLLAMERLTAEIDRSDRADGAISALLFVDVQG
                     LKRVNDSYGHSFGDRLLVAIGMRLLERIGRHDMVARFGGDEFLLLLARRSDVGAVRRF
                     AKELVKAFTSAIDIQGQQVHTSITVGGALAPLDGRDADTLLRCADTARSHAKAEGGST
                     CRFYDESMRRRALETAQLDNLLRGALERNEFSLAYQPVVDLASGRPIGAEALLRWNSP
                     VLGPVSPDRFIQLAEETSLIVPIGEWVLEQACRELAAWREMLNLPLHMAINVSSRQFH
                     DYRFVDRVQTILQNHELPGDAIVLELTERLFLGDDGTSIEVMRELAEQGVRIAIDDFG
                     TGYSSLSYLSRFPVHLVKIDRAFVKGLPEDSESAGLSAAIVAMAGALGLEVICEGVET
                     PEQQTYLAGLGAQHGQGWLFGKPLPSADIRQLLLNRASSAPAA"
     gene            complement(3988..4731)
                     /gene="cysZ"
                     /locus_tag="PK144_09710"
     CDS             complement(3988..4731)
                     /gene="cysZ"
                     /locus_tag="PK144_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HNF66043.1"
                     /translation="MISPFLRGARCLLTGLSLLGHERLRSFVLLPLVINLLLFGGGLW
                     WGGSELSAWLDAQIAKLPDWLHWLHWLLVPLLVAAAALVTFYGFSIVANLVGAPFNGW
                     LAERAEALFDPAAQVPERPLWQEVTIAFASALRATLYFLLRAIPLLLLFFIPVVNIAA
                     PFIWAAFGAWMLALQYLDYPLGNHGIDFSEQRRAHRADRARTFGFGCAVLLLTLVPVL
                     NFVAMPAAVIGATALAVERRRTAGVAALR"
     gene            4998..5714
                     /gene="tolQ"
                     /locus_tag="PK144_09715"
     CDS             4998..5714
                     /gene="tolQ"
                     /locus_tag="PK144_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNF66044.1"
                     /translation="MTAEMSFTHLILQASWVVQLVMLLLLLISLASWTVIFRKRIALS
                     HARRQADTFEDDFWSGVDLAALYTRVSRREDEAEGLEAVFLAGFQEFLRLRAQTGVDP
                     ESVVAGSQRAMRAALSRETDELELQLPVLATAASVSPYIGLFGTVWGIMNAFMGLGAA
                     KTATLQQVAPGIAEALIATAIGLFAAIPAVIAYNRYSTEVDRLVNRYDTFAEEFSNIL
                     QRQAHSLRPRQPQAEVHGPA"
     gene            5701..6129
                     /gene="tolR"
                     /locus_tag="PK144_09720"
     CDS             5701..6129
                     /gene="tolR"
                     /locus_tag="PK144_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNF66045.1"
                     /translation="MARRSSRKKSVSEINVVPLIDVMLVLLIIFMVTAPLLNQGVEVD
                     LPKGVAEALPQQEQEPMVLTVDREGHLYLNRADDPKLALSTEDLLIRARAVLQREPKT
                     VVLVKGDKGVEYGKVVAAMHLLTQAGAAKVGLSMDLPDGA"
     gene            6137..>6983
                     /gene="tolA"
                     /locus_tag="PK144_09725"
     CDS             6137..>6983
                     /gene="tolA"
                     /locus_tag="PK144_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNF66046.1"
                     /translation="MGPDLVSPKPHHRSPRQAGRLRSLVLSVALHGSLLALALLTLSP
                     HVMPPPPAAPKPPESGEIIQATVVDEAKVAAEVQRLEAAERQKREAEAARQRQLDQQA
                     RAAEERRAREEQRLKDLQARQEEAARRAEAEQRRQEEATRRAEAEQRRQEDAARRAEA
                     ETKQRLAQLAQQQQEAERKAQEARAKADADARAARVAEERREAEEKRAADAKAKAEAE
                     ARARAEAARKAEAEKKKLAAEAEAREKAEDEAREKAEAAKKAAAAARAKADADAKAKA
                     DADAKA"
     assembly_gap    6984..7083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7084..7488
                     /gene="tolA"
                     /locus_tag="PK144_09730"
     CDS             <7084..7488
                     /gene="tolA"
                     /locus_tag="PK144_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNF66047.1"
                     /translation="AEAKARADAEAKAAAARKDLADLLAREAKERAAQQQVTAAALSA
                     YQSLLVNHVEPRYQPPPACPEGGVADISIKLSPGGEVVSASVRRSSGNAVCDGAALAA
                     LKRASPLPLPRDPVLAERLQQESFVFKFKVAE"
     gene            7500..8792
                     /gene="tolB"
                     /locus_tag="PK144_09735"
     CDS             7500..8792
                     /gene="tolB"
                     /locus_tag="PK144_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNF66048.1"
                     /translation="MLKTLPTRARAFAALLLTLAAVTTARAELTIEIVKGVEGALPIA
                     IAPFGGNAGLPADIAAIITADLKRSGRFSPLASSQYADRPTELSQVNYASFRSIGVPH
                     LAVGQVRAGGAGYAVQFQLADVYQGSQLLGSSFDVRSGDLRRVAHHIADLIYEKLTGE
                     KGAFNTRIAYVTARGTSSYTLVVADSDGYNPQVVLRSPLPVMSPAWSPDGSKLAYVSF
                     ENRAAQIVVQNVFTGARQTISASPGINGAPAWSPDGSQLAFTLSKDGNPEIYRYDLAS
                     RQTQRLTSTSAIDTEATWSPDGGSIVFTSDRGGGPQLYRMPAGGGGAERLTFSGDYNS
                     RPTFSPDGKSIAYVTRSGGRFHIAVMDASGRNTRLLTDGGMDESPSFAPNGGMILYAT
                     QQGGRGVLAAVSTDGRVRQTLLSTDGEVREPAWSPYGR"
     gene            8852..9430
                     /gene="pal"
                     /locus_tag="PK144_09740"
     CDS             8852..9430
                     /gene="pal"
                     /locus_tag="PK144_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNF66049.1"
                     /translation="MNAERIPTPKNRSLQTLKTVLAAATLATLAACSSTGSTSGDGSS
                     GSGGSGTGAYGASAGGQYGSGGAYGSGGPAGGYVGGPGATQEQRVVYFDYDSADIRAD
                     SRPIIQAHAQYLQQTPGSVAILEGHADERGSREYNIALGERRSVAVRRAMEALGVQPN
                     QMRTVSYGEERPAVGGHDESAYAQNRRVEIAY"
     gene            9445..>9663
                     /locus_tag="PK144_09745"
     CDS             9445..>9663
                     /locus_tag="PK144_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66050.1"
                     /translation="MTAAALRRLALATALLAALPAGAQQRDQSSLIYELLDRINQLED
                     EVRQLRGDVEMLRYRQEQGAHAQPAPAAP"
BASE COUNT         1515 a         3546 c         3265 g         1237 t
ORIGIN      
        1 tgaagcccgc ccccggccgg caggccgctc cgctggctcc ggtgccgcgg ggcggtcacg
       61 gccgatgagc tgtctcccgc gcggacgcat caccgtcgcg tccgccgtcc ttccgccgcc
      121 acgagcggcc cagcgagagt gatgatccgc atgagcacca acgccgacct gatgcttcgc
      181 cgcaatgccg cgctgccgcg cggtgtcggt caggccttcc cgatcttcat cgagcgcgcg
      241 cgcaatgccg aggtctggga cgtcgagggc cgccgctaca tcgacttcgg cggtggcatc
      301 gccgtgctca acgtcggcca caaccacccg cgcgtggtcg ccgcggtgca ggcgcaggcc
      361 gaaaagctcg cacacacctg cttcatggtc accgcctacg aggactacgt gacggtctgc
      421 gagcggctga acgcgctgtc gacgattccc ggggccaaga gcttcctggt cagcaccggc
      481 gccgaagcgg tcgagaacgc cgtcaagatc gcgcgggcgt acacgaagcg tccggcggtg
      541 atcgccttcg gcggcggctt ccacggccgc acgctgctcg gcatgggcct gaccggcaag
      601 gtcgcgccgt acaaggccgg cttcggaccc ttcccgggcg gcctctatca cgccgcctac
      661 ccgtgcccgc tgcacggcgt gtcgatcgac gacgcgctcg cctcggtcga gaagctcttc
      721 aagtacgacg tcgatccgca gacggtcgcc gcgatcatcc tcgaaccggt gcagggcgaa
      781 ggcggcttct acatcgcgcc gccggccttc ctgcagaagc tgcgcgcgct ctgcgatgcg
      841 cacggcatcc tgctgatcgt cgacgagatc cagaccggcg ccgggcgcac cggtacctgg
      901 ctcgcgatcg agcagtcagg cggcatcgtg cccgacctga tcacgatggc caagtcgatc
      961 ggcggcggtt atccggtcgc ggccgtcatc ggccggcccg aggtcatgga cgcgccgaac
     1021 cccggcggcc tcggcggcac ctacgccggc aacccggtgg cctgcgccgc ggcgcgcgcg
     1081 gtgctcgacg tgttcgccga cgagcacctg ctcgatcgcg cccgccagct cggcgagcac
     1141 ctgcgcgccc ggctcgaagc gctgaagacg cgcttcgcga gcatcgccga ggtgcgcggc
     1201 ctcgggccga tggtcgcctt cgaactctgc accgacgccg atccggcgcg cccggccgcc
     1261 gagctgacga aggcgctgac ggcgctcgcc gccgagcgcg ggctgatcct gctggcctgc
     1321 ggcgtctacg gcaacgtcat ccgcatcctc gtgccgctga ccgccgaacc ggcgatcgtc
     1381 gacgagggcc tggacatcct cgccggctgt ctggagacgc tcggcgcctg agccgctccc
     1441 ccgcaccggc cggccccgtc tccccgaccc ggccgaccgg tgcggcatct tccgcaaagc
     1501 agcattgatc gaggtcgtta cagactcatt cgtcgctgtc ccgcccattt tccttatgga
     1561 taatggccgt tcgcccatca cctccttcac ataaaggagc cgccgggccg gcggcagtcg
     1621 ggagacgtgc tcatgaagtc caaggtcgcc tccgccctgc cgcaactgct gcttgcgctg
     1681 ctggtgctcg ccatcgtcgc cagcctcggc gtcgccggcc tgcgcctgcg cgaacgactg
     1741 aacgccccgc cccccgatac gccgaacgcc gcacacgcac tggcgagcac gaccgagcgc
     1801 gcgctgctgc gcgtcgcgct gacgatcggc cggctgcaga ccggcgaggc accggtcgcg
     1861 ctgcgcgagg ctcacgcgca gctcgcggcc gtgctcaccg aggccggcgc cagcaccccg
     1921 gcggcgagcc agctgctggc gctgccggac gccgccgccg cctggggcga ggcgcgcgga
     1981 ctcgccgatg aactcgcacc gctggtgaag ctgccgcagc tcgacgcgcc gctgctcgcc
     2041 ggacgcttcg tgccgcggct cgacgccacc gccgcgcgca tcggcaaggt ggccgatgcc
     2101 gccgacgccg ccagccgcca gcccgacccg gaccgcaacg ccgacctgcg cgccctgatc
     2161 gcgctcgcgc tcgcgggcct cgccgggctg ctgctcgccg gcacgctggc gcatcgccag
     2221 tcgcagctcg cgcggctgcg cgagcaggtc gcgcacggca tcgccgaacg caccgtcggc
     2281 cagcagcaga cctccgagcg gctggtgacg ctgtcgcacg cgatcgagca gagcccggcc
     2341 atcgtgctga tcgccgatgc cagcggctgc gtcgaatacg ccaatccgcg cttcgagacc
     2401 gtgaccggct atccggtcag cgagatcctc ggcaagcgcg tcggcacgct gccgaccgac
     2461 cggctcgatg ccgacgagcg cgcgcagatg tgggccgacc tgtcggccgg caagatctgg
     2521 cagcgcgaga gcgaatcgcg cacgcgccgc gacgatccgt actgggagcg cacgcagatc
     2581 gcgccggtgc gcgatctgca gggcgagctg acgcacttcg tcgtggtcaa ggaggatgtc
     2641 agcgagcgca agcagttcga ggcgaagctc ctgcgtcagg cgcgctacga cgaactgacc
     2701 ggcctgccga accggctgct ggcgatggag cgcctcaccg ccgagatcga ccgctccgac
     2761 cgggccgacg gcgcgatctc ggcgctgctg ttcgtcgacg tgcagggcct caagcgcgtc
     2821 aacgacagct acggccacag cttcggcgac cgcctgctgg tggcgatcgg catgcgcctg
     2881 ctcgaacgca tcggccgcca cgacatggtc gcgcgcttcg gcggcgacga gttcctgctg
     2941 ctgctggcac ggcgcagcga cgtgggtgcc gtgcgccgct tcgcgaagga gctggtcaag
     3001 gccttcacgt cggcgatcga catccagggc cagcaggtgc acacctcgat cacggtcggc
     3061 ggcgcgctgg cgccgctcga cggccgcgat gccgacacgc tgctgcgctg cgccgacacc
     3121 gcgcgcagcc acgccaaggc cgagggcggc agcacctgcc gcttctacga cgagagcatg
     3181 cgccggcgcg cgctggagac cgcgcagctc gacaacctgc tgcgcggggc gctggagcgc
     3241 aacgagttct cgctcgcgta ccagccggtc gtcgatctcg ccagcggccg gccgatcggc
     3301 gcagaggcgc tgctgcgctg gaacagcccg gtgctcggcc cggtgtcgcc ggatcgcttc
     3361 atccagctcg ccgaggaaac cagcctgatc gtgccgatcg gcgaatgggt gctcgaacag
     3421 gcctgccgtg aactggcggc ctggcgcgag atgctgaacc tgccgctgca catggccatc
     3481 aacgtctcgt cgcgccagtt ccacgactac cgcttcgtcg accgcgtgca gacgatcctg
     3541 cagaaccacg agctgccggg cgatgcgatc gtgctggagc tgaccgagcg gctgttcctc
     3601 ggcgacgacg gcacctcgat cgaggtcatg cgcgaactcg ccgagcaggg cgtgcgcatc
     3661 gcgatcgacg acttcggcac cggctattcg tcgctgagct acctgagccg cttcccggtg
     3721 catctggtca agatcgaccg cgccttcgtc aagggcctgc ccgaagactc cgagagcgcc
     3781 ggactcagcg ccgcaatcgt cgcgatggcc ggcgcgctcg gcctcgaagt catctgcgaa
     3841 ggcgtcgaga cccccgagca gcagacctat ctcgccggcc tcggcgcgca gcacggtcag
     3901 ggctggctgt tcggcaagcc gctgccttcg gccgacatcc gccagctgct gctcaaccgc
     3961 gcgtcgagcg cgccggcggc ctgagcttca gcgaagcgcg gcgacgcctg ccgtgcgccg
     4021 gcgctcgacc gccagcgccg tcgccccgat caccgctgcc ggcatcgcga cgaagttcag
     4081 caccggcacc agcgtcagca gcagcaccgc gcagccgaag ccgaaggtgc gggcgcggtc
     4141 ggcacggtgc gcgcggcgct gttcgctgaa gtcgatgccg tgattgccga gcgggtagtc
     4201 gaggtactgc agcgccagca tccacgcgcc gaacgccgcc cagatgaaag gtgcggcgat
     4261 gttgacgacc gggatgaaga acagcagcag cagcgggatc gcgcgcagca ggaagtacag
     4321 cgtcgcgcgc agcgcgctgg caaacgcaat cgtcacctcc tgccacagcg gccgttccgg
     4381 cacctgcgcc gcgggatcga acagcgcctc ggcgcgctcg gccagccagc cgttgaacgg
     4441 tgcgccgacc agattggcga cgatgctgaa tccatagaag gtcaccagcg ccgccgcggc
     4501 gacgagcagc ggcaccagca gccagtgcag ccagtgcagc cagtcgggca gcttcgcgat
     4561 ctgcgcatcg agccaggccg acagctcgct gccgccccac cacaggccgc cgccgaacag
     4621 cagcaggttg atcacgagcg gcagcagcac gaagctgcgc aaccgctcgt ggccgagcag
     4681 gctcaggccg gtcagcaggc agcgcgcgcc gcgcaggaag ggactgatca tcgggattcg
     4741 ctcatgcagc gggacgggag gtgtgcggct gcgacagcgg gccgcggaac tttacgccgc
     4801 cggcgccgtt ctgccgtcgc atcgtgctga tacaatgctg caccgcattc aagcgtgccg
     4861 gtagtccggt gcgccgcgcc cttccccccg tgcatcggat gcccgccccg gaacttcccc
     4921 ggcgggccgg ccgcattgtc cggcgcggag ccggtcgcgc cgccgtcccg acacgcgccg
     4981 ttccggagta ggttgcgttg accgccgaga tgtccttcac acacctgatc ctgcaggcga
     5041 gctgggtggt gcagctggtg atgctgctgc tgctgctgat ctcgctggcc tcatggacgg
     5101 tgatcttccg caagcgcatc gcgctcagcc acgcccgccg tcaggccgac accttcgagg
     5161 acgacttctg gtccggcgtc gacctcgccg cgctgtacac gcgcgtcagc cgccgcgagg
     5221 acgaggccga gggcctcgaa gccgtgttcc tcgccggttt ccaggagttc ctgcgcctgc
     5281 gcgcgcagac cggcgtcgat ccggaatccg tggtcgccgg cagccagcgc gcgatgcgcg
     5341 cggcgctgtc gcgcgagacc gacgagctcg aactgcagct gccggtgctc gcgaccgcgg
     5401 cctcggtcag cccgtacatc ggcctgttcg gcacggtctg gggcatcatg aacgccttca
     5461 tggggcttgg tgccgccaag accgcgacgc tgcagcaggt cgcgcccggc atcgccgagg
     5521 cgctgatcgc gaccgcgatc ggcctgttcg cggcgattcc ggcggtgatc gcgtacaacc
     5581 gctactcgac cgaggtcgat cgcctcgtca accgctacga caccttcgcc gaggagttct
     5641 cgaacatcct gcagcgtcag gcgcacagcc tgcgcccgcg ccagccgcag gccgaagtcc
     5701 atggcccggc gtagctcgcg caagaaatcg gtcagcgaga tcaacgtcgt gccgctgatc
     5761 gacgtgatgc tggtgctgct gatcatcttc atggtcacgg cgccgctgct gaaccagggc
     5821 gtcgaggtcg acctgccgaa aggcgtcgcc gaagccctgc cgcagcagga gcaggagccg
     5881 atggtgctga ccgtcgatcg cgaggggcat ctgtacctga accgcgccga cgacccgaag
     5941 ctcgcgctga gcaccgagga tctgctgatc cgtgcccgtg ccgtgctgca gcgcgagccg
     6001 aagaccgtcg tgctggtcaa gggcgacaag ggcgtcgaat acggcaaggt ggtcgcagcc
     6061 atgcacctgc tgacccaggc cggggccgcc aaggtcggcc tgtcgatgga cctgcccgac
     6121 ggtgcctgag cacgacatgg gtcctgatct cgtgagcccg aaaccgcacc accgctcgcc
     6181 ccgccaggcc ggccggctgc gctcgctcgt gctgtcggtg gcgctgcacg gctcgctgct
     6241 ggcactggcg ctgctgacgc tgagcccgca tgtgatgccg ccgcccccgg ctgcgccgaa
     6301 gccgccggag tcgggcgaga tcatccaggc caccgtcgtc gatgaggcga aggtcgctgc
     6361 cgaggtccag cgcctcgaag ccgccgagcg ccagaagcgc gaggccgagg ccgcacgcca
     6421 gcgccagctc gaccagcagg cacgcgccgc cgaggagcgg cgcgcccgcg aggaacagcg
     6481 gctgaaggat ctgcaggccc gccaggagga agccgcccgt cgtgccgaag ccgagcagcg
     6541 ccggcaggag gaagccacac gccgggccga ggccgagcag cgccggcagg aggatgccgc
     6601 gcgtcgcgcc gaagccgaga cgaagcagcg cctcgcgcag ctcgcccagc agcagcagga
     6661 ggccgaacgc aaggcgcagg aagcccgcgc caaggccgac gccgacgcca gagccgcccg
     6721 cgtcgccgag gagcgccgcg aggccgagga aaagcgtgcc gccgatgcga aggcaaaggc
     6781 cgaagccgaa gccagagcgc gcgccgaggc cgcgagaaag gccgaggccg aaaaaaaaaa
     6841 gctagcggct gaggccgagg cccgcgagaa ggccgaagac gaggcccggg agaaagccga
     6901 agcggcgaag aaggcggccg cagccgccag ggcgaaggcc gacgccgatg caaaggccaa
     6961 agccgatgcc gatgcgaagg ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnngccgagg ccaaggccag ggcggatgcc gaagccaagg ccgcggcagc gcggaaggat
     7141 ctcgccgacc tgctcgcacg cgaagccaag gagcgcgccg cgcagcagca ggtcacggcc
     7201 gcagcactca gtgcctacca gagcctgctg gtcaaccacg tcgagccgcg ctaccagccg
     7261 ccgccggcct gccccgaggg cggggtcgcg gacatttcga ttaagctgtc gccgggcggc
     7321 gaggtcgtct cggccagcgt gcggcgcagc agcggcaatg ccgtctgcga cggcgccgca
     7381 ctcgccgcac tgaagcgcgc ctcgccgctg ccgctgccac gcgatcccgt gctggccgag
     7441 cggctgcagc aggaaagctt cgtgttcaag ttcaaggttg ccgagtgagg aagcgcaggg
     7501 tgctcaagac tctcccgacg cgcgcgcgtg cctttgccgc gctgttgctg acgctggccg
     7561 ccgtgaccac ggcccgcgcc gaactgacca tcgagatcgt caagggtgtc gaaggggccc
     7621 tgcccattgc gattgcgccg ttcggcggca acgccgggct gccggccgac attgccgcga
     7681 tcatcacggc cgatctcaag cgcagcggcc gcttctcgcc gctcgcatcg agccagtacg
     7741 ccgaccgccc gaccgagctg tcgcaggtca actacgcgag cttccgctcg atcggcgtgc
     7801 cgcacctcgc ggtcggtcag gtgcgcgccg gcggggccgg ctacgccgtg cagttccagc
     7861 tcgccgatgt ctatcagggc tcgcagctgc tcggcagcag cttcgacgtg cgtagcggcg
     7921 acctgcggcg cgtcgcgcac cacatcgccg acctgatcta cgagaagctc accggcgaga
     7981 agggcgcgtt caacacgcgc atcgcctacg tcaccgcgcg cggcaccagc agctacacgc
     8041 tggtggtcgc cgattccgac ggctacaacc cgcaggtcgt gctgcgctcg ccgctgccgg
     8101 tgatgtcgcc ggcctggtcg ccggacggca gcaagctcgc ctacgtcagc ttcgagaacc
     8161 gcgccgcgca gatcgtcgtg cagaacgtat tcaccggcgc tcgccagacc atctcggcct
     8221 cgcccggcat caacggcgcg ccggcctggt cgccggacgg cagccagctc gcgttcacgc
     8281 tgtcgaagga cggcaacccc gagatctacc gctacgacct cgcctcgcgc cagacgcagc
     8341 gcctgaccag cacctcggcg atcgacaccg aggccacgtg gtcgccggac ggcggctcga
     8401 tcgtgttcac gtccgatcgc ggcggcggtc cgcagctcta ccggatgccg gcgggcggcg
     8461 gcggcgccga gcgcctgacc ttctccggcg actacaactc gcggccgacc ttctcgccgg
     8521 acggcaagag catcgcctac gtcacgcgca gcggcggacg cttccacatc gccgtcatgg
     8581 acgcgagcgg ccgcaacacc cgcctgctga ccgacggcgg catggacgaa tcaccgagct
     8641 tcgcgccgaa cggcggcatg atcctctacg ccacgcagca gggcgggcgc ggcgtgctcg
     8701 cggccgtgtc caccgacggc cgcgtcaggc agacgctgct gtcgaccgac ggcgaagtcc
     8761 gcgaacccgc gtggtcgccg tacggccgct gaaccgcagc cctccgatcc gccccgatcc
     8821 cgaagaaccc gcacaggaat ccgatccgaa catgaacgcc gagcgcatcc cgaccccgaa
     8881 gaaccgctcc ctgcagacgc tgaagaccgt gctcgccgcc gccacgctgg caacgcttgc
     8941 cgcctgctcc tcgaccggca gcacctccgg cgatggttcc agcggcagcg gcggcagcgg
     9001 caccggagcc tacggcgcca gtgccggcgg ccagtacggc agcggcggtg cctacggcag
     9061 cggcggcccg gccggcggct acgtcggcgg ccccggcgcc acgcaggagc agcgcgtcgt
     9121 gtacttcgac tacgacagcg ccgacatccg cgccgactcg cggccgatca tccaggcgca
     9181 cgcgcagtac ctgcagcaga cgccgggctc ggtggcgatc ctcgaaggcc acgccgacga
     9241 gcgcggcagc cgcgaataca acatcgccct cggcgagcgg cgcagcgtcg cggtgcgtcg
     9301 cgccatggaa gcgctcggcg tgcagccgaa ccagatgcgc acggtcagct acggcgagga
     9361 gcgcccggcc gtcggcggcc acgacgagag cgcctacgcg cagaaccgtc gcgtcgagat
     9421 cgcctactga gccgcgccgc cgccatgacc gccgccgcgt tgcgccgcct cgccctcgcg
     9481 accgccctgc tcgccgcgct gcccgccggc gcccagcagc gcgaccagtc ctcgctgatc
     9541 tacgagctgc tcgatcgcat caaccagctc gaagacgaag tccgccagct gcgcggcgac
     9601 gtcgagatgc tgcgctaccg ccaggaacag ggcgcgcacg cccagccggc gccggcagcc
     9661 ccg
//
LOCUS       DAOQIE010000134         9598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3618_length_9598_cov_7.395264, whole genome shotgun sequence.
ACCESSION   DAOQIE010000134 DAOQIE010000000
VERSION     DAOQIE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_9598_cov_7.395264"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PK144_09750"
     CDS             complement(<1..173)
                     /locus_tag="PK144_09750"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF66051.1"
                     /translation="MTDASTTRPDPATAHAARLFLQSAGRRYPLRLALLFGSRARGDF
                     HVGSDADIAVLRDG"
     gene            complement(305..1774)
                     /locus_tag="PK144_09755"
     CDS             complement(305..1774)
                     /locus_tag="PK144_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNF66052.1"
                     /translation="MTTSFPPAAAAGLAPLLMPAVAELVPPAWAGYRPLIVEALDGFL
                     GRLSGARLDAIVAAQLELPEDATAAARVVALARACPSLHKLGQVLARDRRLDAQLRVR
                     LQTLESLPPETPAEVWLPLIRAEIGAVPHLEIAPQALAEASVAIVVPFARHGGIAPDA
                     RGVFKVLKPGIEAQLAEELAIWAELGDWLEARSAELGLPELPYRETLSGVQGLLLNEI
                     RPETEQAHLAAACRRHAEDPNVLIPQPLEYCTPRLTAMTRIDGDKITEATGHRPRLAR
                     ALYAALLAQPFWQPQAQTMFHADPHAGNLRVTADGRLAILDWSLVAVLDREARIALVR
                     VVLAALAQDGALFARALAALGRPVREDALRALSAAALAEVRTGRLPGFDWLLSVLDRA
                     ATTRALEFPESLVLLRKALFTLAGVVAELDADCGAVPLLLKHAFDARSFDPRGFGPHV
                     FAGDLGTLWLSGLPIAARWWAGYWQDWSGGRLPFLPVPG"
     gene            1930..2670
                     /locus_tag="PK144_09760"
     CDS             1930..2670
                     /locus_tag="PK144_09760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF66053.1"
                     /translation="MTMNHDDPLLPAGPQPLNRDSYDAIASAWDAARRCLDEREAGWL
                     LDLVRGLRPPAPVLDLGCGSGRPLGEWLLARGLQVTGIDQSARLLALARTRLPQGRWL
                     EADIGGWIPDTDTDTDTDTDTDTDTDTDKNFAAVLIWDALFHLPRALHLPLLARIFRL
                     LQPGGRLLLSSGGSAQPAFTDTMHGARFFYDALAPDVLLAGLLDLGFGLERIEYLNPP
                     TRGRDKGRIAVLARRPAAAPATVPHAAE"
     gene            2819..5545
                     /gene="acnA"
                     /locus_tag="PK144_09765"
     CDS             2819..5545
                     /gene="acnA"
                     /locus_tag="PK144_09765"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNF66054.1"
                     /translation="MTHDPFDVLRVFTTASGQHGRYCSLAALESAGLGAVSRLPVSLR
                     LVLESVLRHCDGENITEAHVRALAGWQPQAPRSEEIPFVVARVVLQDFTGVPLLCDLA
                     AMRDAAAAAGGEPGIVEPLVAVDLVVDHSVMIDHWGRPDALELNLQREYERNRERYAF
                     LKWGTQALRSFRVVPPGTGIVHQVNLEYLCRGVHERDGITYPDTLVGTDSHTTMIDAL
                     GVLGWGVGGIEAEAAMLGQPLYFLTPDVVGVELTGRLREGVTATDLVLAVTERLRRER
                     VVGCFVEFCGEGTAGLSVTDRATIANMAPEYGATTGFFPVDARTLEYLQHTGRSDEEI
                     ERFEAYFRAQGLFGVPAAAELDFSRLVTIDLAAVVPSLAGPKRPQDRIALDELPARFR
                     QLLAGRPDEGGYGLTAEQIDARVPLAGGGEPAENREDALPADLGHGDVLIAAITSCTN
                     TSNPEVMIAAGLLARAAVGRGLTVRAGIKTSLAPGSRVVTDYLERAGLLAPLAELGFA
                     LSAYGCTTCIGNAGELAPAIEQAVAGHGLVCAAVLSGNRNFEARIHPRIRANFLGSPP
                     LVVAYALAGRIDIDLAHEPLGHDADGRPVFLRELWPARAEIADTLAHASDPDSYRRRY
                     GAIDGGPLWQDIPVPNGARYHWPDSAYIARPPFFDGFTRTPSPPADIRDARALLILGD
                     SVTTDHISPAGSFRADSPAGRYLQSLGVPPAEFNSYGSRRGNHEVMIRGTFANVRIRN
                     RMLAPGADGKPVEGGWTLLDGERVTVYDAAMEYGRRGVPTLVFAGEEYGTGSSRDWAA
                     KGTLLLGVRAVVARSYERIHRSNLVGMGVLPLQFSGEDSAHTLNLGPDDRFDLPPLAS
                     LKPQQTLELTIRSPDGSVRQVPVLCRIDTPIELTCFRHGGILPWVLRQLLDD"
     gene            5595..6989
                     /gene="fumC"
                     /locus_tag="PK144_09770"
     CDS             5595..6989
                     /gene="fumC"
                     /locus_tag="PK144_09770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNF66055.1"
                     /translation="MNMRIEHDSLGDVEVPDERLWGAQTQRSLEHFRISTEKMPPELI
                     RALARLKRAAALANAGLGVLPEPVAQAIVQAAGEIIDGGHADEFPLAVWQTGSGTQTN
                     MNLNEVIANRASELLGGPRGQGRKVHPNDAVNAGQSSNDAFPSAMHLAAVEAVHRQVL
                     PALATLRETLADKAVACAGVIKIGRTHLQDATPLTLGQEMSGWVAQLAHAERHLTDAL
                     PHLCELALGGTAVGTGLNAHPQFAERAIAELAHSTGLPFVPAPNRFEALAAHDALVHA
                     HGVLKSLAAALVKIANDVRWLASGPRSGLGELTIPENEPGSSIMPGKVNPTQCEALTM
                     ACAQVFGNDVAIGFAGSQGNFELNVYKPLIIHAFLQSTRLLADGMDSFRRHCALGIEP
                     NLPRIRELLDRSLMLVTALNRHIGYDRAAAIARKAHTEGLTLKEAALALGFVSAEQFD
                     EWVRPEQMLGPGTD"
     gene            7007..7429
                     /locus_tag="PK144_09775"
     CDS             7007..7429
                     /locus_tag="PK144_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF66056.1"
                     /translation="MHQAIRIRELDHVVLRVRDPARMQHFYETVLGCPVERRLDIGLI
                     QLRAGASIIDLVPFDSELGRQGGHAPDHDGPNLDHFCVRVEPWDEAAIRAHLAAHGLA
                     PGPTERRYGAEGMGPSIYLRDPEGNVVELKGPPDGQSA"
     gene            7584..8849
                     /locus_tag="PK144_09780"
     CDS             7584..8849
                     /locus_tag="PK144_09780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNF66057.1"
                     /translation="MSVAAREACSVRRFRVRSCATLLLATLPPVAAADVVGIDASIFW
                     LLVSVMAITGLAALFVQQRMQRRLDRAERGLQRAVSEQREAERRYRLLAAHGSELIWT
                     MSLGTPQFTYLSPALERLCQFAPSSMQNRPPEELCTLESAALLSAEHARLSRQAGDAS
                     IPEVRFELELRCADGTTLWTDTRLGVLRDESGTATAIYGVVSDMTEQRKLRARLDFLA
                     HHDALTGLPNRMLFFDRLERAVAAARRDGTRLALLYLDLDGFKEINDSLGHPAGDTLL
                     MQVALRLSASVRDSDTVARMGGDEFTVILRTVRDAPSVARITEKILTALSEPIAIDAQ
                     RVRIGVSIGASQFPEHGDQVGTLVALADAAMARSKRGGRNRWSMAIPDTPVLKSVPVE
                     APPAETPPPEGAGSVRPVLAAVRNPLSES"
     gene            8980..9564
                     /locus_tag="PK144_09785"
     CDS             8980..9564
                     /locus_tag="PK144_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNF66058.1"
                     /translation="MPRLPRLLSALAFAAVAHGAQAIPINYVATLSGAAEAPPNASAG
                     TGSAHVVIDTDAHTYAIHFDFSGLTGTTTAAHIHGPTASPGTGTAGVMTTTPAFPGFV
                     TGVSSGSYDQLFDMTVASTFNPAFVTAQGGLANAEAAFAAALASGSAYLNIHSSFVTA
                     GEIRGFLTQIPEPASLGLLAIGALGLGRLRARRR"
BASE COUNT         1403 a         3565 c         3305 g         1325 t
ORIGIN      
        1 ccgtcccgca gcaccgcgat gtcggcgtcg ctgccgacgt ggaaatcccc ccgcgcccga
       61 ctgccgaaca acagggccag ccgtagcgga tagcgtcggc cagcggactg gaggaaaagt
      121 cgagcggcat gtgccgtggc ggggtccggc cgtgtagtgg aagcatccgt catcatcgcc
      181 cgcctccccg tcgtccccgg ttgcctgccc atcaccagac gcagccggtt ccccggcgtt
      241 taccacatcg tgcgggccgg actgtcatcc gtgcaaatcc tgccgcacgc cgccgcccgg
      301 cacctcagcc cggcaccggc aggaacggca gccggccgcc gctccagtcc tgccagtaac
      361 cggcccacca gcgggccgcg atcggcagcc cggacagcca cagcgtgccg agatcgcccg
      421 cgaacacgtg cgggccgaag ccgcgcgggt cgaaggaacg ggcgtcgaag gcgtgcttca
      481 gcagcagcgg cacggcgccg cagtctgcat cgagttcggc gacgacgccg gcgagcgtga
      541 acagcgcctt gcgcagcagc accagcgact ccggaaactc cagcgcacgc gtcgtcgcgg
      601 cccggtcgag caccgacagc agccagtcga agcccggcag ccggccggtg cgcacctcgg
      661 cgagcgcggc cgccgacagc gcgcgcaggg cgtcttcccg gaccgggcgg ccgagcgcgg
      721 cgagcgcgcg cgcaaacagc gcgccgtcct gcgcgagcgc cgcgagcacg acgcgcacca
      781 gcgcgatgcg cgcctcgcga tcgagcacgg ccaccagact ccagtcgagg atcgcgagcc
      841 gcccgtcggc ggtcacacgc aggttgccgg cgtgcgggtc ggcatgaaac atcgtctgcg
      901 cctgcggctg ccagaacggc tgcgcgagca gcgccgcgta cagcgcgcgg gccaggcgcg
      961 ggcgatggcc ggtggcttcg gtgatcttgt cgccgtcgat gcgcgtcatc gccgtcagcc
     1021 gcggcgtgca gtattcgagc ggctgcggga tcagcacgtt cgggtcttcg gcgtggcggc
     1081 ggcaggcggc ggcgaggtgc gcctgttcgg tctcggggcg gatctcgttg agcagcagcc
     1141 cctgcacgcc gctgagcgtc tcgcggtacg gcagttcggg caggccgagc tcggcgctgc
     1201 gcgcttccag ccagtcgccg agttcggccc agatcgcgag ttcctcggcg agctgcgcct
     1261 cgatgccggg cttcagcacc ttgaacacgc cgcgcgcatc cggcgcgatg ccgccgtggc
     1321 gcgcaaacgg cacgacgatc gcgacgctgg cctcggccag tgcctgcggc gcgatctcca
     1381 gatgcggcac cgcaccgatc tcggcgcgga tcagcggcag ccagacctcg gccggcgtct
     1441 cgggcggcag cgattccagc gtctgcagcc gcacgcgcag ctgcgcatcg agccggcggt
     1501 cgcgcgccag cacctgcccg agcttgtgca ggctcgggca ggcacgcgcg agcgcgacca
     1561 cgcgcgctgc ggccgtcgcg tcctcgggca gttcgagctg cgcagcgacg atggcatcga
     1621 gccgcgcgcc cgacagccgc ccgaggaagc cgtcgagcgc ctcgacgatc agcggccggt
     1681 atccggccca ggccggcggc accagttcgg cgaccgcggg catcagcagc ggcgcgaggc
     1741 cggcggcagc ggccggcggg aaggaggtgg tcatgtcggc attccctgaa atggcgcatc
     1801 gcatgcgata cggaagtgaa aacagcgcag cgctgcgggt tcccggacgg attttccgcc
     1861 cgtgcagccg gggccatcga tcgcggcccc ggcagcggcc atgctgaagg caggaatcag
     1921 gccgcaatca tgacgatgaa ccatgacgat ccgctgctgc cggccgggcc gcagccactg
     1981 aaccgcgaca gctacgacgc catcgccagc gcctgggatg ctgcgcgccg ttgcctggac
     2041 gagcgcgaag ccggctggct gcttgatctg gtgcgcgggc tgaggccgcc ggcaccggtg
     2101 ctcgatctcg gctgcggcag cggccggccg ctgggggaat ggctgctggc gcgcgggctg
     2161 caggtcaccg gcatcgatca gtctgcccgc ctgctggcgc tggcccgcac ccggctgccg
     2221 cagggacgct ggctggaagc cgacatcggg ggctggattc cggacacgga cacggacacg
     2281 gacacggaca cggacacgga cacggacacg gacacggaca agaatttcgc cgcggtgctc
     2341 atctgggatg cgctgtttca cctgccgcgt gccctgcacc tgccgctgct ggcccgcatc
     2401 ttccgtcttc tgcagcccgg tggccggctg ctgctcagct cgggcggcag cgcgcagccg
     2461 gccttcaccg acaccatgca cggcgcgcgc ttcttctacg acgcgctggc gccggatgtg
     2521 ctgctcgcag ggctgctcga tctcggcttc ggactcgaac gcatcgagta cctgaatcca
     2581 ccgacccgcg gccgcgacaa gggccgcatc gccgtgctcg cgcgcaggcc cgctgcagcg
     2641 ccggcaacgg ttccccacgc tgccgaatag cagcgcttcc ggagcaggcg gcggacgcct
     2701 ctcctacgct gatcgacaga ccgatcgcaa gcccttgagc ccgcagccgg gaatccgtcg
     2761 accgctgcgc agtcgcaggt tgcaggcctg tccgatccgt tgccggagaa tccttccgat
     2821 gacccacgat ccgttcgacg tgctgcgcgt cttcacgacc gcctccggcc agcatggccg
     2881 ttactgcagc ctcgccgcgc tcgagagcgc cggtctcggc gccgtgtcgc ggctgccggt
     2941 gtcgctgcgt ctggtgctgg aatcggtgct gcgccactgc gatggcgaga acatcaccga
     3001 ggcgcacgtg cgcgccctgg ccggctggca gccgcaggcg ccgcgcagcg aggaaatccc
     3061 gttcgtggtc gcgcgcgtcg tgctgcagga cttcaccggc gtgccgctgc tctgcgatct
     3121 ggcggcgatg cgcgatgccg cggccgccgc cggcggcgag cccggcatcg tcgagccgct
     3181 ggtcgcggtc gatctggtcg tcgatcactc ggtgatgatc gatcactggg gccggcccga
     3241 tgcgctcgaa ctgaacctgc agcgcgaata cgaacgcaac cgcgagcgct acgccttcct
     3301 gaagtggggc acgcaggcct tgcgcagctt ccgcgtcgtg ccgcccggca ccggcatcgt
     3361 ccatcaggtc aacctcgaat acctgtgccg cggcgtgcac gagcgcgacg gcatcaccta
     3421 cccggacacg ctggtcggca ccgattcgca cacgacgatg atcgacgcgc tcggcgtgct
     3481 cggctggggc gtcggcggca tcgaggccga ggcggcgatg ctcggccagc cgctgtactt
     3541 cctgacgccg gacgtggtcg gcgtcgagct gaccggccgg ctgcgcgagg gcgtgaccgc
     3601 caccgatctg gtgctggccg tgaccgagcg cctgcgcagg gagcgcgtgg tcggctgctt
     3661 cgtcgagttc tgcggcgagg gcacggcggg cctgtcggtc accgaccgcg cgaccattgc
     3721 caacatggcg cccgaatacg gcgcgacgac cggcttcttc ccggtcgatg cgcgcacgct
     3781 cgagtacctg cagcacaccg ggcgcagcga cgaggagatc gagcgcttcg aggcctactt
     3841 ccgcgcccag ggcctgttcg gcgtgccggc ggcggccgag ctcgacttct cgcggctcgt
     3901 gaccatcgat ctcgcggccg tcgtgccgag cctcgccggc ccgaagcgcc cgcaggaccg
     3961 catcgcgctc gatgaactgc cggcgcgctt ccgccagctg ctcgccggcc ggccggacga
     4021 aggcggctac gggctgacgg cggagcagat cgatgcgcgc gtgccgctcg caggcggcgg
     4081 cgagccggcc gagaaccgcg aggacgcgct gccggccgac ctcggccacg gcgacgtgct
     4141 gatcgccgcg atcacctcgt gcacgaacac ctcgaacccg gaggtgatga tcgccgccgg
     4201 cctgctcgca cgtgccgccg tgggccgtgg cctgacggtg cgtgccggca tcaagacctc
     4261 actcgccccc ggctcgcgcg tggtcacgga ttacctcgaa cgcgcggggc tgctggcgcc
     4321 gctcgccgaa ctcggctttg cgctgtcggc ctacggctgc acgacctgca tcggcaacgc
     4381 cggcgaactc gcgccggcga tcgagcaggc cgtcgccggg cacgggctgg tgtgcgcggc
     4441 ggtgctctcc ggcaaccgca acttcgaggc gcgcatccac ccgcgcatcc gcgccaactt
     4501 cctgggctcg ccgccgctgg tggtcgcgta tgcgctggcc gggcgcatcg acatcgacct
     4561 cgcgcacgag ccgctcggcc acgatgccga cggccggccg gtgttcctgc gcgaactctg
     4621 gccggcgcgc gccgagatcg ccgacacgct cgcgcacgcg agcgacccgg acagctaccg
     4681 ccgccgctac ggcgcgatcg acggcgggcc gctgtggcag gacatcccgg tgccgaacgg
     4741 cgcgcgctac cactggccgg actcggccta catcgcccgg ccgccgttct tcgacggctt
     4801 cacgcgcacg ccctcgccgc cggccgacat ccgcgatgcg cgcgcgctgc tgatcctcgg
     4861 tgactcggtg accaccgacc acatctcgcc ggccgggagc ttccgcgccg actcgccggc
     4921 cgggcgctac ctgcagtcgc tcggcgtgcc gccggccgag ttcaacagct acggctcccg
     4981 gcgcggcaac cacgaggtca tgatccgcgg caccttcgcg aacgtgcgca tccgcaaccg
     5041 gatgctggcc cccggtgcgg acggcaagcc ggtcgagggc ggctggacgc tgctcgacgg
     5101 cgagcgggtg acggtctacg acgcggcgat ggaatacggc cggcgcggcg tgccgacgct
     5161 ggtgttcgcc ggcgaggaat acggcaccgg ttcgagccgc gactgggcgg ccaagggcac
     5221 gctgctgctc ggcgtgcgtg cggtggtcgc gaggagttac gagcgcatcc accgctcgaa
     5281 cctggtcggc atgggcgtgc tgccgctgca gttcagcggc gaggacagcg cccacacgct
     5341 gaacctcggc ccggacgacc gcttcgacct gccaccgctc gcgagcctca agccgcagca
     5401 gacgctggaa ctgacgatcc gcagccccga tggcagcgtg cgccaggtgc cggtgctctg
     5461 tcgcatcgac acgccgatcg aactcacctg cttccgccac ggcggcatcc tgccctgggt
     5521 gctgcgccag ctgctcgacg actgagggcg gtcacgagca gaggccggac ggctgcttcc
     5581 ccggagtgat cccgatgaac atgcgcatcg aacacgacag cctcggcgac gtggaagtcc
     5641 ccgacgaacg gctctggggc gcacagacgc agcgctcgct ggagcacttc cgcatctcga
     5701 ccgagaagat gccgccggag ctgatccggg cactggcgcg cctcaagcgc gccgccgcgc
     5761 tcgccaacgc cgggctcggc gtgctcccgg agcccgtcgc gcaggcgatc gtgcaggcgg
     5821 ccggcgagat catcgacggc ggccatgccg atgaatttcc gctcgcggtc tggcagaccg
     5881 gctccggcac gcagaccaac atgaacctca acgaggtgat cgccaaccgc gccagcgagc
     5941 tgctcggcgg cccgcgcggc cagggccgca aggtgcaccc gaacgatgcg gtcaacgccg
     6001 gccagtcctc gaacgacgcc ttcccgagcg cgatgcacct cgctgcggtc gaggccgtgc
     6061 accggcaggt gctgccggcg ctggcgacgc tgcgcgagac gctcgccgac aaggccgtcg
     6121 cctgcgccgg cgtcatcaag atcggccgca cgcacctgca ggacgccacg ccgctgacgc
     6181 tcgggcagga gatgtcgggc tgggtcgcgc agcttgcgca cgccgaacgc catctgaccg
     6241 atgccctgcc gcacctctgc gaactcgcgc tcggcggcac cgcggtcggc accggcctca
     6301 acgcccatcc gcagttcgcc gaacgcgcga tcgcggaact ggcgcacagc accggcctgc
     6361 ccttcgtgcc ggcaccgaac cgcttcgagg cgctggccgc gcacgatgcg ctggtgcacg
     6421 cgcacggcgt gctgaagtcg ctggccgcgg cgctggtcaa gatcgccaac gacgtgcgct
     6481 ggctcgcctc ggggccgcgc tcgggcctcg gcgagctcac gatcccggag aacgagccgg
     6541 gcagctcgat catgcccggc aaggtcaacc cgacgcagtg cgaggcgctg acgatggcct
     6601 gcgcgcaggt gttcggcaac gacgtggcga tcggcttcgc cggctcgcag ggcaacttcg
     6661 agctgaacgt ctacaagccg ctgatcatcc atgccttcct gcagagcacg cggctgctcg
     6721 ccgacggcat ggacagcttc cgccgccact gcgcgctcgg catcgaaccg aacctgccgc
     6781 gcatccgcga gctgctcgac cgttcgctga tgctggtgac ggcgctgaac cgccacatcg
     6841 gctacgaccg cgccgccgcg atcgcacgca aggcgcacac cgagggcctg acgctgaagg
     6901 aggccgcgct ggcactcggc ttcgtcagcg ccgaacagtt cgacgaatgg gtgcgcccgg
     6961 agcagatgct cggccccggt actgactgag gaacgggagc cgcacgatgc atcaggccat
     7021 ccgcatccgt gaactcgatc acgtcgtgct gcgcgtgcgc gatccggcac gcatgcagca
     7081 cttctacgaa accgtgctcg gctgcccggt cgagcggcgg ctcgacatcg gcctgatcca
     7141 gctgcgcgcc ggtgcctcca tcatcgatct ggtgccattc gacagcgaac tcggccgtca
     7201 gggcgggcac gcgccggatc acgacggccc gaacctcgat cacttctgcg tgcgcgtcga
     7261 gccctgggac gaggccgcga tccgcgcgca tctggccgcc cacggcctcg cgcccggccc
     7321 gaccgaacgc cgctacggcg ccgaaggcat ggggccgtcg atctacctgc gcgacccgga
     7381 aggcaacgtc gtcgagctga agggtccgcc ggacgggcag agcgcctgag gtctgcagcc
     7441 ctcggcgcgc cggccggccg ctcatgcgaa atttcccgtt accggctgcg gatgttcgta
     7501 cgcttctggc atggtagcga cacccgtccc gtgccggtcc gccacggggt ccagaccacc
     7561 gtgttcgccg aggccgccgc ccgatgtccg ttgccgccag ggaagcctgc tccgtccgcc
     7621 gattccgggt ccgcagctgc gcgacactgc tgctcgccac cctgccgccg gtggcggcgg
     7681 ccgatgtggt cggcatcgat gcctcgatct tctggctgct ggtctcggtc atggcgatca
     7741 ccggtctggc ggcgctgttc gtgcagcagc gcatgcagcg ccggctcgac cgtgccgaac
     7801 gcgggctgca gcgcgccgtc agcgagcagc gcgaggccga acgccgttac cggctgctgg
     7861 ccgcgcacgg cagcgagctg atctggacga tgtcgctcgg gacgccgcag ttcacgtatc
     7921 tgagcccggc cctcgaacgg ctgtgccagt tcgcgccatc gagcatgcag aaccggccgc
     7981 cggaggaact ctgcacgctc gaatcggctg cgctgctgag cgccgaacac gcccggctga
     8041 gccgtcaggc cggcgatgcg tcgattccgg aagtccgctt cgaactcgaa ctgcgctgcg
     8101 ccgacggcac gacgctctgg accgatacac gcctcggcgt gctgcgcgac gagagcggga
     8161 ccgcgactgc gatctacggc gtcgtcagcg acatgaccga gcagcgcaag ctgcgtgccc
     8221 ggctcgattt cctcgcccac cacgacgcgc tgaccgggct gccgaaccgc atgctgttct
     8281 tcgaccggct ggagcgcgcg gtcgccgccg cccgccgcga cggcacccgg ctggcgctgc
     8341 tgtacctcga cctcgacggt ttcaaggaga tcaacgacag cctcggccac cctgccggcg
     8401 acacgctgct gatgcaggtg gcgctcaggc tctcggccag cgtccgcgat tccgacacgg
     8461 tcgcgcgcat gggcggggac gagttcacgg tgatcctgcg cacggtgcgc gacgccccct
     8521 cggtcgcgcg catcaccgag aagatcctca ccgcgctgtc cgaaccgatc gcgatcgacg
     8581 cccagcgcgt gcgcatcggc gtcagcatcg gcgccagcca gtttccggag cacggcgatc
     8641 aggtcggcac gctggtggca ctggccgatg cagcgatggc gcgcagcaag cgcggcggtc
     8701 gcaaccgctg gagcatggcg atcccggata cgccggtgct gaagtcggta ccggtggaag
     8761 ccccgcctgc cgaaacaccg ccaccggagg gcgccggcag cgtgcgtccg gtgctggccg
     8821 cggtacgcaa tcccctgtcc gaatcgtaag ccggccgccc cggctgccct gcttccgctg
     8881 cgactgccgg cgactacgct ggcgctgtgc cggatcacac cctgccggca catgcccgtg
     8941 gaacccagtc aacaaaagcc atcagggagt gccttgccca tgccccgcct tccccgcctg
     9001 ttatccgccc tcgccttcgc cgccgtcgcc catggtgcgc aggccatccc gatcaactac
     9061 gtcgcgacgc tgagcggtgc cgccgaagca ccgccgaatg cctcggccgg caccggcagc
     9121 gcgcacgtgg tcatcgatac cgatgcgcat acgtacgcga tccacttcga cttcagcggc
     9181 ctgaccggca cgaccaccgc cgcccacatc cacggcccga ccgcctcgcc cggtaccggc
     9241 acggccggcg tgatgacgac gaccccggcg tttccgggct tcgtgaccgg cgtcagctcg
     9301 ggcagctacg accagctctt cgacatgacg gtggcctcga ccttcaaccc tgccttcgtg
     9361 accgcgcagg gcggcctcgc caatgccgaa gcggcgtttg ccgccgcgct cgcctccggc
     9421 agcgcctatc tgaacatcca ctcgagcttc gtgaccgccg gcgagatccg cggcttcctg
     9481 acgcagatcc cggaaccggc cagcctcggc ctgctggcga tcggtgccct cgggctcgga
     9541 cggctgcgcg cgcgccggcg ctgatcgccg cgcccggccc ccggccagag gccccggt
//
LOCUS       DAOQIE010000135         9581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3623_length_9581_cov_7.393345, whole genome shotgun sequence.
ACCESSION   DAOQIE010000135 DAOQIE010000000
VERSION     DAOQIE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3623_length_9581_cov_7.393345"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PK144_09790"
     CDS             <1..675
                     /locus_tag="PK144_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF66059.1"
                     /translation="VRIAVHEAVRGRGIGRALLATLADAAARDGFDLLGTSFSAETGV
                     LRFWQAAGLRPVRVGSGHDAASGAPPLMLLRALSAAGQAMLAPLLARFATDLPLRLAG
                     PLSALEAELAAALLAGAGAAAGVDTAHDAATVTAFAHGARDWASAWPALWRAAGRALA
                     EAGSPLTPAERALLVAALLQHQPWPALARLIDASGRAAVETRLRQVFARLADHAGPAP
                     AAARGR"
     gene            complement(748..2484)
                     /locus_tag="PK144_09795"
     CDS             complement(748..2484)
                     /locus_tag="PK144_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNF66060.1"
                     /translation="MQPSDRLILLAGLLMLVSVITTRLSRRIGMPILLVFLGFGMLAG
                     EDGPGGLAWSDVPTSHFIGSLALAVILFDGGMRTQLKSFRVGLRPALGLATLGVLFSA
                     AITGLFAAWALGFDYVEGLLIGAIVGSTDASAVFGVLHAHGLRLKERVGATLEIESGL
                     NDPMAIFLTVAVLEWLLAGRPALDAAPLLLFAQQMGVGAVLGLAGGYLVWLLLDRLDL
                     GAVFTPLFAFAAGLALYGTTAFFGGSGFLAVYLGGLVIGNRPSVGLSLVLRLHEGLAW
                     LAQIAMFLILGLLVTPSQIVPVAWPALLVGLGLMFAARPLAVWLCLLPFRFSPGERLF
                     IAWVGLRGAVPIVLAMFPLLADLPHAGTMFNVAFVVVLLSLSVQGWTVAPLARRLGLE
                     LPPAVTPQRHVVSELPGTPGAVFASYVVAVNAPVAGGVLGTLGLPPASRPVAVARGGR
                     LLAEPASVRLVAGDAVHLLAPPGDLEVLDRQFGGRPQRPGPAERAFFGEWVLNGDANL
                     EALAGLYGLQLAADERTLTVGALLTRAFRKPVVGDRLRRGRLEIVVRELDGDRVLRVG
                     LKLHRNGETVAG"
     gene            complement(2675..3292)
                     /locus_tag="PK144_09800"
     CDS             complement(2675..3292)
                     /locus_tag="PK144_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF66061.1"
                     /translation="MNAVVCPRLTVADYLAGERDAELRHEYENGFVRAMTGTSRAHNS
                     IAMNVAAVLHSHLRGSPCRVHVSDVKVHVDLADDQRFYYPDVLVDCGSGGDAYVASAP
                     KLVVEVLSASTERRDRAEKAQGYRRLPTLAEYVLIAQDVRRVEVYRRSEGWEFERTID
                     SAAAPVRFESLGLELTLDAIYEGLELPADPGDSPKPAHGSPDSAG"
     gene            complement(3464..3889)
                     /locus_tag="PK144_09805"
     CDS             complement(3464..3889)
                     /locus_tag="PK144_09805"
                     /inference="COORDINATES: protein motif:HMM:NF019460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HNF66062.1"
                     /translation="MPSTLFVCTTCRRADNVDDATTESCGSKLKRELEAAAESLPEAA
                     RPKIEGYECLMNCERACNVAIRAQGKSGFLLGGFAPVLADARALLDYTWLYNASPGGV
                     VPYRDWPDGVRGRFCGRIPAASADPLPQARSEPALEGEK"
     gene            complement(4171..4701)
                     /locus_tag="PK144_09810"
     CDS             complement(4171..4701)
                     /locus_tag="PK144_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNF66063.1"
                     /translation="MPAPESVGVSEVLRLRAEPLTQAAFAPFGDVIEPAAASRHYPIN
                     AGSAERFHDVMRIDTGTADGHTVLSLVFGQPVTLPARLRLLERHPLGSQSWLPLDGQR
                     FLIVVAPAGEQWALADVRVFVSDGRQGVNYARGVWHHPLLSLGETPGRYVVVDRDGPG
                     DNCEERPLPRVVELVI"
     gene            complement(4701..5714)
                     /gene="alc"
                     /locus_tag="PK144_09815"
     CDS             complement(4701..5714)
                     /gene="alc"
                     /locus_tag="PK144_09815"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNF66064.1"
                     /translation="MTATAQQQAPDWTRQYPNLASARLGARALTVSDEWFAPRERLLD
                     DAEAVFIPGKYDDHGKWMDGWETRRKRVTGHDWCIVRLAVPGTVYGVDIDTSHFTGNF
                     PPAASIDGCCLAEGGDPDADTVWQPLLAAVSLGGDAHHFHVLDARLPVTHVRLNIFPD
                     GGVARLRVHGRALAGDTAPGAELELSSMLLGGRIVACNNSHYGPPHALLAPGRGVNMG
                     DGWETRRRREPGNDWVIVELARPGRITRIEVDTAHFKGNYPDACSLQAACAGAGTDQS
                     MITQSMFWPELLPAQKLSADAIHHFDAEALAALGPVTHVRFNIHPDGGVSRLRVFGRV
                     ESV"
     gene            complement(5863..6792)
                     /gene="puuE"
                     /locus_tag="PK144_09820"
     CDS             complement(5863..6792)
                     /gene="puuE"
                     /locus_tag="PK144_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNF66065.1"
                     /translation="MSDQPYPRDLIGYGRTPPHARWPQQARIAVQFVLNYEEGGENCV
                     LHGDAGSEQFLSELFAPASFPDRHLSMESIYEYGSRVGVWRILREFERRGLPLTVFGV
                     AMALERHPEVTTAFVELGHEIACHGWRWIHYQNIDEATEREHLRIGLEVIEHLTGTRP
                     LGWYTGRDSPNTRRLVVDDGGLLYDSDYYGDDLPFWTEVTRSDGVTMPHLIVPYTLDA
                     NDMRFSLPQGFSHGDEFFEYLRDSFDVLYAEGAETPRMLSVGLHCRLIGRPGRFRALQ
                     RFLDHIERHDRVWVCRRIDIARHWHAEHPYVPG"
     gene            complement(6810..7544)
                     /locus_tag="PK144_09825"
     CDS             complement(6810..7544)
                     /locus_tag="PK144_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNF66066.1"
                     /translation="MRICVINPNTSAAMTETIGRAARRVAAAGTEIVAQQPDAGPESI
                     ESHVDEAFAAVGVAELVRRGELLGFDGYVLACFGDPGLLAARELAKGPVLGIAEAAFH
                     AAAMLAGRFSVVTTLARTVPIAEHLLHAYGLERRCVRVRAAEIPVLDLDTPAAYARIA
                     GECRRARDEDGIGALVLGCGGMAELAPELSRELGLPVVDGVTAAVKFVESLIALGLGT
                     GKTGDGAYPPPKRYVGRFADWTTTAC"
     gene            complement(7913..8668)
                     /locus_tag="PK144_09830"
     CDS             complement(7913..8668)
                     /locus_tag="PK144_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNF66067.1"
                     /translation="MNVKTASVLPLGGLETGPRERGRDEEIYRSLYTAIVEHHLAPGA
                     RLPEDALAESFGISRTGIRKVLQRLAHERLIELAPNRGATVAQPSVQEAREVFAARRI
                     VECAALTEIGREPPPVAALDELKRIVAAETDAQARGDKREAIHLSGLFHLRVLALADN
                     RPLSDFLGQLVSRTSLVIAMYGTPLAAGCNRCRHEQLIELLAAGDTAAAARWMDEHLR
                     EIEAGCAFAESEPQATDLKRIFAEIAKRPRSEV"
     gene            9238..>9581
                     /locus_tag="PK144_09835"
     CDS             9238..>9581
                     /locus_tag="PK144_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNF66068.1"
                     /translation="MSHPLPPGYSDRLYNEDLAPLGEGKNWNWYNIFAFWMSDVHSVG
                     GYIFAGSLFALGLSSWQVLVSLLVGICLVNVLCNLVAKPSQQAGVPYPVACRMSFGVW
                     GANIPAIIRGLIA"
BASE COUNT         1525 a         3413 c         3304 g         1339 t
ORIGIN      
        1 gtgcgcatcg ccgtccacga ggccgtgcgc gggcgcggca tcggccgggc gctgctcgcg
       61 acgctggccg acgccgccgc gcgcgacggc ttcgatctgc tcggcaccag tttctcggcc
      121 gaaaccggcg tgctgcgctt ctggcaggcg gcggggctgc ggccggtgcg cgtcggcagc
      181 ggtcacgatg ccgcgagcgg tgccccgccg ctgatgctgc tgcgcgcgct gtcggcagcc
      241 gggcaagcga tgctcgcgcc gctgctcgcg cgcttcgcga ccgacctgcc gctgcggctc
      301 gccggaccgc tgtcggcgct ggaagcggag ctggccgcgg cgctgctcgc cggcgccggt
      361 gccgcggccg gcgtcgatac cgcccacgac gcggcgaccg tcaccgcctt cgcacacggc
      421 gcacgcgact gggcgagtgc ctggccggcg ctgtggcgcg ccgcgggccg ggcgctcgcg
      481 gaggccggtt caccgctgac gcccgccgaa cgcgcgctgc tcgtggccgc gctgctgcag
      541 caccagccct ggccggccct cgcgcggctc atcgacgcga gcggccgggc cgcggtcgaa
      601 acgcggctgc ggcaggtgtt cgcccggctc gcggatcacg ccgggccggc cccggcagcg
      661 gctcgcggac gctgaccggg acgccggctc ctgcccgccg cccggaccgg gggcagtcga
      721 aagaaggcaa gcgccggccc cgcgccttca gccggccacc gtctccccgt tgcggtgcag
      781 cttcaggccg acgcgcagga cgcgatcgcc gtcgagttcg cgcacgacga tctccagccg
      841 cccgcgtcgc agccggtcgc cgaccacggg cttgcggaag gcgcgcgtca gcagcgcacc
      901 gaccgtcagc gtgcgttcgt cggcggcgag ctgcaggccg tacagcccgg ccagtgcttc
      961 cagattcgca tcgccgttga gcacccattc accgaagaac gcccgctcgg ccggaccggg
     1021 gcgctgcgga cggccgccga actggcgatc gagcacttcg agatcaccgg gcggggcgag
     1081 caggtgcacg gcatcgccgg cgacgaggcg cacgctcgcc ggttcggcca gcagccggcc
     1141 gccgcgggcc accgccaccg gccggctggc cggcggcagg ccgagcgtgc cgagcacacc
     1201 accggcgacc ggcgcgttga cggcgacgac gtagctcgcg aacaccgcgc ccggcgtgcc
     1261 cggcagctcc gagaccacat ggcgctgcgg cgtcaccgcc ggcggcagct ccagtccgag
     1321 ccggcgcgcc agcggtgcga ccgtccagcc ctgcaccgac agcgacagca gcacgacgac
     1381 gaaggcgacg ttgaacatcg tgccggcatg cggcaggtcg gcgagcagcg ggaacatcgc
     1441 cagcacgatc ggcaccgccc cgcgcagccc cacccaggcg atgaacagcc gctcgcccgg
     1501 cgagaagcgg aagggcagca ggcagagcca taccgccagc ggccgggcgg cgaacatcag
     1561 cccgaggccg acgaggagcg ccggccacgc caccggcacg atctgcgacg gcgtcaccag
     1621 caggccgagg atcaggaaca tcgcgatctg tgccagccac gcgagcccct cgtgcaggcg
     1681 cagcaccagc gacaggccga ccgaaggccg gttgccgatc accagcccgc cgagatagac
     1741 ggcgaggaag ccgctgccgc caaagaacgc cgtcgtgccg tacagcgcca gcccggccgc
     1801 gaacgcaaag agcggcgtga acaccgcgcc gagatcgagc cggtcgagca gcagccagac
     1861 cagataaccg ccggcgagcc cgagcacggc gccgacgccc atctgctgcg cgaacagcag
     1921 cagcggcgcg gcatcgaggg ccggccggcc ggcgagcagc cattcgagca cggcgacggt
     1981 caggaagatc gccatcgggt cgttgaggcc ggattcgatc tccagcgtcg cgccgacgcg
     2041 ctccttcaga cgcaggccgt gcgcgtgcag cacgccgaac accgccgagg cgtcggtcga
     2101 gccgacgatc gcgccgatca gcaggccctc gacatagtcg aagccgagcg cccaggcggc
     2161 gaacaggccg gtgatcgcgg cactgaacag cacgccgagc gtcgccagcc cgagcgccgg
     2221 gcgcaggccg acgcggaagc tcttgagctg cgtgcgcatg ccgccgtcga acaggatcac
     2281 ggcgagcgcc agactgccga tgaagtggct ggtcggcacg tcgctccagg ccaggccgcc
     2341 ggggccgtcc tcgccggcga gcatgccgaa gccgaggaac accagcagga tcggcatgcc
     2401 gatcctccgg ctcagccggg tggtgatgac gctgaccagc atcagcaggc cagcgagcag
     2461 gatcaggcga tcgctcggtt gcacgggtcg attccgcaga aggagggaaa cgggcgccga
     2521 cccccgcgtt caggcgccgg ccatctgcgg gtgcagcata cggaagaccg tgcggcgcgc
     2581 cggtgacgcg ccactcatta aattatggac agaaccccgg acaggccctg caacggcccg
     2641 ccggcatcct cgccggcgcc tgcgcgcccc gcactcagcc ggcggaatcc gggctgccgt
     2701 gcgcgggctt cggcgagtct cccggatcgg ccggcagctc cagcccctcg tagatcgcat
     2761 cgagcgtcag ttcgagaccg aggctttcga agcgcaccgg cgcggcggcg ctgtcgatcg
     2821 tgcgctcgaa ctcccagcct tcgctgcgcc ggtagacctc gacgcggcgc acgtcctgcg
     2881 cgatcagcac gtactcggcg agcgtcggca gccgccggta gccctgggcc ttctcggcac
     2941 ggtcacgccg ctcggtcgag gccgacagca cctcgacgac cagcttcggt gccgacgcca
     3001 cataagcatc accgccgctg ccgcagtcga cgagcacgtc cgggtaatag aagcgctggt
     3061 cgtcggcgag atcgacgtgg accttgacgt cggagacatg cacacgacac ggcgagccgc
     3121 gcagatgact gtgcagtacc gccgcgacat tcatggcgat gctgttgtgc gcccggctgg
     3181 tgccggtcat cgcccgcacg aagccgttct cgtactcatg ccggagttcg gcatcgcgct
     3241 cgccggcgag gtaatcggcg accgtcaggc ggggacagac gactgcgttc atggcgatgg
     3301 acctctgctg ccggacgcga ctgacctgca gtctcgacgc cgatgcccgc agaggcaagc
     3361 ctgcagatcg gaaagaaaaa acccgctgca cggacagcgg gttgcgatca ggagcgtgag
     3421 gccactcccg gattcacgga cgccggccgg cgccggccgc tgcttacttc tcgccttcca
     3481 gcgccggctc ggagcgggcc tgcggcagcg gatcggcgct cgcggccggg atgcgaccgc
     3541 agaagcggcc gcgcacgccg tccggccagt cgcgataggg cacgacaccg cctggcgagg
     3601 cgttgtacag ccacgtgtag tcgagcagcg cgcgcgcatc ggcgagcacc ggcgcgaagc
     3661 cgccgagcag gaagccgctc ttgccctgcg cgcggatcgc gacgttgcag gcgcgctcgc
     3721 agttcatcag gcattcatag ccttcgatct tcggacgggc ggcctcgggc aggctctcgg
     3781 ctgcggcttc cagctcgcgc ttgagcttgc tgccgcagga ctcggtggtc gcatcatcga
     3841 cgttgtcggc gcgacggcag gtggtgcaga cgaacagcgt ggacggcatc agcacacctc
     3901 cgcacaacgg cgtgccggac gcaaacagac cctgagcttg cgcatcgctg gaaccctctt
     3961 cccgtgcttg acgtttatcg ttgttggaat gcagccggat tcgctgagac cggcgcagcc
     4021 gcactggacg gacgcatcag ccgcggcggc cgggtggcgt ccgtggccga cggaagcatg
     4081 ccgggctggt gcgtgccgga gtatccacgt ccggcgcgcc gcagcaagcc cccgcgctgc
     4141 gctgccgcag cgcgcgcagg gtgaaatgcc tcagatcacg agttccacca ctcgcggcag
     4201 cgggcgttcc tcgcagttgt cgccgggacc gtcgcgatcg acgacgacgt agcggcccgg
     4261 cgtctcaccg agcgacagca gcgggtgatg ccagacgccg cgcgcgtagt tgacgccctg
     4321 gcggccgtcg ctgacgaaca cgcgcacgtc cgcgagcgcc cactgctcgc cggccggcgc
     4381 gaccacgatc aggaagcgct ggccatcgag cggcagccag ctctggctgc cgagcggatg
     4441 gcgttcgagc agcctgagcc ttgccggcag cgtcaccggc tggccgaaga ccaggctcag
     4501 caccgtgtgc ccgtctgcgg tgccggtgtc gatgcgcatg acatcgtgga agcgctcggc
     4561 gctgccggcg ttgatcggat agtgccggct ggcggcggcc ggctcgatca cgtcgccgaa
     4621 cggcgcgaac gccgcctgcg tcagcggctc ggcgcgcagg cgcagcactt cggaaacccc
     4681 gacggactcc ggagccggca tcacaccgac tccacgcgcc cgaacacgcg caggcggctg
     4741 acaccgccgt ccggatggat gttgaagcgc acgtgcgtga ccgggccgag cgcggccagg
     4801 gcttcggcat cgaagtgatg gatcgcgtcg gccgacagct tctgtgccgg cagcagctcg
     4861 ggccagaaca tcgactgcgt gatcatcgac tggtcggtgc ctgcccctgc gcaggcggcc
     4921 tgcagcgagc aggcgtccgg gtaattgccc ttgaagtgcg cggtatcgac ctcgatgcgc
     4981 gtgatccgcc cgggccgcgc cagctcgacg atgacccagt cgttgccggg ctcgcgccgc
     5041 cggcgcgttt cccagccatc gcccatgttg acgccgcgcc ccggcgcgag cagcgcatgc
     5101 ggcgggccgt agtggctgtt gttgcaggcg acgatgcgcc cgccgagcag catcgacgac
     5161 agctccagct ccgcgcccgg cgccgtgtcg ccggcaagcg cgcggccgtg cacgcgcagc
     5221 cgcgccacgc cgccatccgg gaagatgttc aggcgcacgt gcgtgaccgg caggcgggca
     5281 tcgagcacgt ggaagtgatg cgcatcgccg ccgagcgaca cggccgcgag cagcggctgc
     5341 cagacggtat cggcatccgg atcgccgccc tcggcgaggc agcagccgtc gatcgacgcc
     5401 gccggcggga agttgccggt gaagtgcgag gtgtcgatgt cgacgccgta caccgtgccc
     5461 ggcacggcca gacgcacgat gcaccagtca tggccggtga cgcgcttgcg gcgcgtctcc
     5521 cagccgtcca tccatttgcc gtgatcgtcg tacttgccgg gaatgaacac ggcctcggca
     5581 tcgtcgagca ggcgctcgcg cggtgcgaac cattcatcgg acacggtcag ggctctggcg
     5641 ccaagtctgg cgctggcgag attcgggtac tggcgggtcc agtcgggggc ttgctgctgt
     5701 gcggtggcgg tcatcgggtg aacctcggga aagacggcgg cggtcgatgg gccgccggcc
     5761 gggggaattc gctgaaacgc tgtgccggca acctccccct ctctccaatc ccctctccgg
     5821 ccaggggcga ggggctgaac agcagacgga tggccggagg aatcagcccg gcacgtacgg
     5881 atgctccgca tgccagtgcc gggcgatgtc gatgcgccgg cagacccaga cacggtcgtg
     5941 gcgctcgatg tgatcgagaa aacgctgcag cgcacggaag cgtccggggc ggccgatcag
     6001 ccggcagtgc aggccgaccg acagcatcct cggcgtctcc gcgccctcgg cgtagagcac
     6061 gtcgaagctg tcgcgcaggt actcgaagaa ctcgtcgccg tgcgaaaagc cctgcggcag
     6121 cgagaagcgc atgtcgttgg catcgagcgt gtacggcacg atcaggtgcg gcatggtcac
     6181 gccgtcgctg cgcgtgacct cggtccagaa cggcaggtcg tcgccgtagt agtcagagtc
     6241 gtacagcagg ccgccgtcat cgacgaccag ccggcgcgtg ttcggcgaat cgcggccggt
     6301 gtaccagccg agcgggcgcg tgccggtcag gtgctcgatg acttccagcc cgatgcgcag
     6361 gtgctcgcgc tcggtggcct cgtcgatgtt ctggtagtgg atccagcgcc agccgtggca
     6421 ggcaatctcg tggccgagtt cgacgaaggc ggtcgtgact tccggatggc gttcgagcgc
     6481 catcgccacg ccgaacacgg tcagcggcag gccgcggcgc tcgaattcgc gcaggatgcg
     6541 ccagacgccg acgcgcgagc cgtattcgta gatcgattcc atcgacagat gccggtccgg
     6601 gaagctcgcc ggcgcgaaca gctcggacag gaactgctcg ctgccggcat cgccgtgcag
     6661 cacgcagttc tcgccgccct cctcgtaatt gagcacgaac tgcacggcga tgcgcgcctg
     6721 ctgcggccag cgcgcgtgcg gcggcgtgcg gccgtagccg atcaggtcgc ggggataagg
     6781 ctggtcggac attacaggct ccgtggcatt cagcacgccg tcgtggtcca gtcggcaaaa
     6841 cggccgacgt aacgcttcgg cggcggatag gcaccgtcgc cggtcttgcc ggtgccgagt
     6901 ccgagcgcga tcagcgactc gacgaacttc acggctgccg tgaccccatc gaccaccggc
     6961 agcccgagtt cgcgcgacag ctccggcgcg agttccgcca tgccgccgca gccgagcacc
     7021 agcgcaccga tgccgtcctc gtcgcgcgcg cgccggcatt caccggcgat gcgtgcatag
     7081 gccgccggag tgtcgagatc gagcaccggt atttcggcgg cacgcacgcg cacgcagcgc
     7141 cgttccagac cataggcgtg cagcaggtgt tcggcgatcg gtacggtacg cgcgagcgtc
     7201 gtgaccaccg agaaacgccc ggccagcatc gccgcggcgt gaaaggccgc ttcggcgatg
     7261 ccgagcaccg ggcctttcgc cagttcgcgc gcggcgagca ggcccggatc gccgaagcag
     7321 gcgagcacgt agccatcaaa accgagcagc tcgccgcggc gcaccagttc ggcgacgccg
     7381 actgcggcga aagcctcatc gacgtgactc tcgatcgact ccggcccggc atccggctgc
     7441 tgcgcgacga tctcggtgcc cgccgccgcg acgcgccggg cggcccggcc gatggtttcg
     7501 gtcatcgcgg cgctggtgtt ggggttgatc acgcagatgc gcatcgggct ggcctcaagg
     7561 gttgatcggg atggaaagga caggctgtgc cggattcagc gtcgtgctga tttccggcgt
     7621 ttccccctct ccccaacccc tcccccgcca ggggggaggg gctttactgc gcaacttccg
     7681 gatcgacaga tgattcgtcg gtggaagtcg aacaatcggt gctgacacga actcgaccga
     7741 ccgcattgcg tcgacggcca aatccaatgc ggacacccga cacaaatgtg cgatcacttc
     7801 ccaaaacacc aacgctgcat ctttcaagcc cctctcccct cgcgggagag gggttgggga
     7861 gagggggaaa aacaccttcc ctacattacg tgcactgcag cgaaaatctg cctcaaacct
     7921 cactccgcgg ccgcttcgca atctccgcaa agatgcgctt cagatcggtc gcctgcggtt
     7981 cggattcggc aaacgcgcag ccggcctcga tctcgcgcaa atgttcgtcc atccagcgcg
     8041 ccgccgcggc cgtatcgccg gcggccagca gttcgatcag ctgctcgtgc cggcagcggt
     8101 tgcagccggc cgcgagcggc gtcccgtaca tcgcaatcac cagcgaggtg cgcgacacca
     8161 gctggccgag gaagtccgac agcggccggt tgtcggcgag tgccagcacg cgcaggtgaa
     8221 acagccctga caggtggatc gcctcgcgct tgtcgccgcg cgcctgcgcg tcggtttcgg
     8281 cggcgacgat gcgcttcagc tcgtccagtg ccgcgaccgg cggcggctcg cggccgattt
     8341 cggtcagggc cgcgcattcg acgatgcgcc gcgccgcgaa cacctcgcgc gcctcctgca
     8401 cgctcggctg cgcgaccgtc gcaccgcggt tcggcgccag ctcgatcagc cgctcgtgcg
     8461 caaggcgctg caacaccttg cggatgccgg tgcggctgat gccgaagctc tcggccagcg
     8521 catcctcggg cagccgcgcg ccgggcgcga ggtggtgctc gacgatcgcc gtatacaggc
     8581 tgcggtagat ctcctcgtcc cgcccgcgct cgcgggggcc ggtttcgagt ccgcccagcg
     8641 gcaggacgga agcggtcttg acgttcatgg cggtctccgt aggccgggca ctgctgccga
     8701 tcctgctcgc cctgccgcgc ggatttccgc gcccctgcct cgccccgctg cggacagggt
     8761 cgggaaacag gccatcccgc acgaccttgc ccgcgatcgg ctcgcccgga cagcacgtcg
     8821 gtttcgatgc gcaaaggcta aaggcttttg tatacaaact caagccttca ttccccgcga
     8881 ccatctgccc aacccgtcgg ctccggcgca cgatccaggc gcgtcgatgc ctgattccag
     8941 agcaggaagc acgactgaat gcaccatgaa tgcgcgacaa atcagtgcgt aagcgcttgt
     9001 ctggtgcatg aatttatcgt catgctgcag ttgcggaatt ttcgcggcat gcctctttac
     9061 ctgcatacgt gtatatgcct tgattgtata cacgtcggca ctgccagacc gacgcgcccc
     9121 gctgcccgac gccgccacga ccgctccccc ggtccgcccg cgcaggcaac gacaccgccc
     9181 ggcgccctcc cgaacccgat cggcgctcgc ggcaaccctg accaggagat tgcccgcatg
     9241 tcccatccgc tcccccccgg ctacagcgac cgcctgtaca acgaggatct cgccccgctc
     9301 ggcgaaggca agaactggaa ctggtacaac atcttcgcct tctggatgtc cgacgtgcac
     9361 agcgtcggcg gctacatctt cgccggcagc ctgttcgcgc tcgggctgtc gagctggcag
     9421 gtgctggtgt cgctgctggt cggcatctgc ctcgtcaacg tgctctgcaa tctggtcgcg
     9481 aaaccgagcc agcaggccgg cgtgccatat ccggtcgcct gccggatgtc gttcggcgtc
     9541 tggggcgcga acatcccggc gatcatccgc ggcctgatcg c
//
LOCUS       DAOQIE010000136         9579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3624_length_9579_cov_7.281814, whole genome shotgun sequence.
ACCESSION   DAOQIE010000136 DAOQIE010000000
VERSION     DAOQIE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3624_length_9579_cov_7.281814"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK144_09840"
     CDS             <1..388
                     /locus_tag="PK144_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNF66069.1"
                     /translation="GDGLAALRESFLLAPCSNGNIALMVALARRNGWHWDAILGAEPA
                     RAYKPQPLVYLHAVAALGLTPAETLMVAAHAADLAAAGALGLRTAFLARAAEYGRPNP
                     ADQPDFPVDFHVDSLPALAAQLRAGG"
     gene            complement(393..3599)
                     /locus_tag="PK144_09845"
     CDS             complement(393..3599)
                     /locus_tag="PK144_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66070.1"
                     /translation="MPGTPLRRLTDEDYLPWLQVGVSFVLIAALVLALGWWFIADYRR
                     EVERRIDQLERETLAQQETLLRRRVDETRIWLDWLRSRAETLLAERVREQAEAAYELV
                     YSMHRLLDGPLGTAETQKLIVETLRPLRFFDGRGYYFIDTLDGDCVLLPTSPEREGHS
                     LLPIRDDNGVCIMCELIRVATQPEGAGLLRYRWYPPNDATRMDDKLTWVRRFEPYRWL
                     LGTGEYLDTMMRMLQREALDRLRALRFEDDGYIAVFHRDGRMMVTPANPDLEGSNGRT
                     TLDATRRAVLEQLLASAGAGGGVVRYDWNRPAGGPLRTKLSVVTPFDAWGWTLVAGLY
                     LDDLDVTLASQRGELEAGVRARERATLWALALALSVGLGSSIAYARWVQARLRARRRE
                     LEASARRLYLTQRCVDAAADSIFLADAGLCVVYANDAACRTLGRPAAAITGRSLGELG
                     LAPPGVPTQPDSYEATPTGPDGRSLALEVAWTPLETGGERYACAIARDVAERKRVEAE
                     LRLASLVFEIGNDAMMITDADNRILAVNPAFTRVTGYAPAEAIGCNPNLLSSGRQPEA
                     FYAALWDALARKGHWSGELWNRRKDGSAYPQWLSISVLRDAQGRAVRHVAAFTDISER
                     KANEARIRHLADYDPLTDLPNRTLLRERIQQTMHAAASGGPGFALVFLDLDHFKHIND
                     TLGHAAGDALLCAVGTRLRSVVRSGDTVSRTGGDEFVLLLPGLVEAADTERLTAKLMR
                     TLAEPYLIGGKELRVTPSLGYACYPHDATDIDTLLGHADAAMYQAKAQGRNNAQPFTP
                     RLSAHLAERFELENALRGALERQELELHYQPQYFVDGRPRGAEALLRWQHPQLGRVSP
                     ERFIPIAEETGLIRPIGDWVLRSACAQAVAWQRAGIPPLTIAVNVSAVQFAQADWVDT
                     VAATLGASGLAPCCLQLELTESVLMKDPAAAALTLKALRATGVRLSIDDFGTGYSSLA
                     YLKRFPLDELKIDRSFIDGLPGDAEDAAIVHAILQLARSLGLETVAEGVETEAQRRFL
                     QAHGCTAVQGWLYAPALPAGAAGERLHPPACRASGRAN"
     gene            complement(3913..4335)
                     /locus_tag="PK144_09850"
     CDS             complement(3913..4335)
                     /locus_tag="PK144_09850"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF66071.1"
                     /translation="MSSPENGTEFPRNRQPTMRTVAMPADTNPYGQIFGGWIMAQMDL
                     AGSVLAIEYAQGRVATVAVHSMEFHQPVTVGDLVSSFARIERIGTTSITVRVEVFAQR
                     PALCVKVTEAVIVYVAVDGEGQRRPLPEHEPAFQSVYG"
     gene            4491..5459
                     /locus_tag="PK144_09855"
     CDS             4491..5459
                     /locus_tag="PK144_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HNF66072.1"
                     /translation="MAKARTVCMYLGEQLGRYGFGDDHPFGEDRIHAFMDEMHRRELD
                     WQVRILDSVACTESELLLFHTPEYVARVQEGSRTGEGMLDYGDTPAYVGVYDHVSHVV
                     GAVLDATHKLMNDQMRRCFVPIAGLHHAMPDTAAGFCVFNDVGVAIKVLQQQYGLERI
                     AYVDIDAHHGDGLFYPFETDPTVIFADIHEDGRYNFPNTGFPHEIGKGDAKGRKLNVP
                     LLPLSGDQEFNMMWEKVEGFVDDWKPQFIFVNCGADGLDGDPLAHLHYSQQAHNRAIR
                     SLCRIAERHSEGRILAVGGGGYDLRNVGRAWATVVNALLESPMTVN"
     gene            5596..6858
                     /gene="glyA"
                     /locus_tag="PK144_09860"
     CDS             5596..6858
                     /gene="glyA"
                     /locus_tag="PK144_09860"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNF66073.1"
                     /translation="MFGKDMQIAGYDDELWAAMQAEAQRQEDHIELIASENYTSPRVL
                     QAQGSVLTNKYAEGYPGKRYYGGCEYVDVAESLAIARAKQLFGADYANVQPHSGSQAN
                     AAVYMALCEPGDTVLGMSLAHGGHLTHGARVNFSGKIYNAVQYGIDDEGRIDYDALAR
                     IAVECRPKMIVAGFSAYSQVLDFARFRQIADQVGAYLFVDMAHVAGLVAAGLYPNPVP
                     FADVVTTTTHKTLRGPRGGLILAKANEAIEKKLNSLVFPGTQGGPLMHVIAAKAVAFK
                     EALEPAFRSYALQVVLNAKAMAGVCLERGYKVVSGGTENHLFLVDLVSHGITGKAADA
                     ALGQAHITVNKNSVPNDPQSPFVTSGIRIGSPAITTRGFKEAEVRQVAHWVCDVLDAV
                     DKPELEATVTRVRGEVAALCARFPVYTH"
     gene            6881..7351
                     /gene="nrdR"
                     /locus_tag="PK144_09865"
     CDS             6881..7351
                     /gene="nrdR"
                     /locus_tag="PK144_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNF66074.1"
                     /translation="MHCPFCGAADTRVVDSRLSPEGDRVRRRRECIACGARFTTFELA
                     ELVMPRVVKRDGRREPFAGDKLRAGMLRALEKRPVGAEAIDAALARITRRVRALGESE
                     VSSNQLGEWMMDELRRLDPVAYVRFASVYRSFEDVNAFREEIERLQAPADGREP"
     gene            7387..8478
                     /gene="ribD"
                     /locus_tag="PK144_09870"
     CDS             7387..8478
                     /gene="ribD"
                     /locus_tag="PK144_09870"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNF66075.1"
                     /translation="MARALALAARGLNTTSPNPRVGCVLVAPDGEVIGEGWHERAGEP
                     HAEVHALRAAGGRARGATAYVTLEPCSHHGRTPPCADALIEAGVARVVAAMADPNPQV
                     AGRGLARLRAAGIAVECGLLEAEAQALNAGFVRRMTRARPWLRLKLAASLDARTALAS
                     GESQWITGEAARADVQRLRARACAVLTGSGTVLADDPGLDVRLPGTQRQPLRVVLDTG
                     LRTPLAARLIGRPGRTLIVCGPQAGVAEAAALAAYGVDIERVPVQGGGLDLDAVLASL
                     AARGCNEVHAECGARLAGALLAAGRVDELVVYLAPLLLGDAARGLARLPGLERMEQRL
                     EFDWTDVRSVGRDLRLTLAPRGPAAPAVS"
     gene            8561..>9579
                     /gene="ribB"
                     /locus_tag="PK144_09875"
     CDS             8561..>9579
                     /gene="ribB"
                     /locus_tag="PK144_09875"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNF66076.1"
                     /translation="MSLNTIEELLTDLRQGRMVLVMDDEDRENEGDLLMIASLTRPED
                     VNFMARYGRGLICLTLTRERCQQLRLPLMVTNNGTPFATNFTVSIEAAEGVTTGISAY
                     DRAHTIRTAVRPDARPEDLVQPGHIFPLMAQTGGVLTRAGHTEAGCDLARLAGYEPAA
                     VICEVLNEDGTMAHRPELERFATEHGIKMGTIADLIRYRLANEPTVERVAECPLPTKH
                     GDFRLYAYQDVLTKQLHYALVKGRPGPADTALVRVHVQNTLGDLFGARIAECGWPLDD
                     ALARIAADPAPGVVIVLARQEEPREMVRRIRHYQVHGPQAARPPAPESAQELRTYGIG
                     AQILI"
BASE COUNT         1424 a         3370 c         3397 g         1388 t
ORIGIN      
        1 cggcgacggg ctcgcggccc tgcgcgagtc cttcctgctg gccccgtgct cgaacggcaa
       61 tatcgcgctg atggtggcgc tggcgcgccg caacggctgg cactgggatg cgatcctcgg
      121 ggccgagccg gcgcgggcct acaagccgca gccgctcgtc tacctgcatg cggtcgccgc
      181 gctcggcctc acaccggccg aaaccctgat ggtcgcggcg catgctgccg atctcgccgc
      241 cgccggcgcg ctcggtctgc gcacggcctt cctcgcgcgt gccgccgaat acggccggcc
      301 gaaccccgcc gaccagccgg atttcccggt cgatttccac gtcgacagcc tgccggccct
      361 ggcggcacaa ctgcgggccg gcggctgaga agctaattcg ccctgcccga ggcccggcag
      421 gccggcgggt gcagccgttc gccggccgcc ccggccggca aagccggggc gtacagccag
      481 ccctggaccg ccgtgcagcc gtgcgcctgc aggaagcggc gctgggcctc ggtctcgacg
      541 ccttcggcga ccgtctccag gccgaggctg cgcgcgagct gcaggatggc gtgcacgatc
      601 gcggcgtcct cggcgtcccc gggcaggccg tcgatgaagc tgcggtcgat cttcagctcg
      661 tcgagcggga agcgcttcag gtacgcgagg ctcgaatagc cggtgccgaa gtcgtcgatc
      721 gacagacgca cgccggtcgc gcgcagcgcc ttcagcgtca gcgcggctgc ggccgggtcc
      781 ttcatcagca cgctctcggt cagttcgagc tgcaggcagc agggcgcgag gccggaagcg
      841 ccgagcgtgg cggcgaccgt atcgacccag tcggcctgcg cgaactgcac ggcggaaaca
      901 ttgacggcga tggtcagcgg cgggatgcct gcccgctgcc aggccacggc ctgcgcgcag
      961 gcgctccgca gcacccagtc gccgatcggc cggatcagtc cggtttcctc ggcgatcggg
     1021 atgaagcgct cgggcgagac gcgcccgagc tgcggatgct gccagcgcag cagcgcctcg
     1081 gccccgcgcg ggcggccgtc gacgaagtac tgcggctggt agtgcagttc cagttcctgg
     1141 cgttcgagcg ccccgcgcag cgcgttctcc agctcgaagc gctcggccag atgcgcgctc
     1201 aggcgcggcg tgaacggctg ggcgttgttg cggccctgcg ccttggcctg gtacatggcc
     1261 gcgtcggcgt ggccgagcag cgtgtcgatg tcggtggcat cgtgcgggta gcaggcgtag
     1321 ccgaggctcg gcgtcacgcg cagctccttg ccgccgatca ggtacggctc ggccagcgtg
     1381 cgcatcagct tggcggtgag ccgctcggtg tcggccgcct cgaccagacc gggcagcagc
     1441 agcacgaact cgtcgccgcc ggtacggctg accgtgtcgc cgctgcgcac gaccgagcgc
     1501 agccgcgtgc cgaccgcgca gagcagcgcg tcgccggcag cgtggccgag cgtgtcgttg
     1561 atgtgcttga aatgatcgag gtcgagaaac accagcgcga agcccggtcc gccgctcgcg
     1621 gcggcgtgca tcgtctgctg gatgcgctcg cgcagcagcg tgcggttcgg caggtcggtc
     1681 agcgggtcgt agtcggcgag gtggcggatg cgggcctcgt tcgccttgcg ttcgctgatg
     1741 tcggtgaacg cggcgacatg gcgcacggcg cgaccttgcg cgtcgcgcag caccgagatg
     1801 ctcagccact gcggataggc cgagccgtcc ttgcgccggt tccagagctc gccgctccag
     1861 tgacccttgc gcgcgagcgc gtcccagagc gcggcataga acgcctccgg ctggcggccc
     1921 gaggacagca ggttcgggtt gcagccgatg gcctcggccg gcgcatagcc ggtcacgcgc
     1981 gtgaaggccg ggttgacggc gaggatgcgg ttgtcggcat cggtgatcat catcgcgtcg
     2041 ttgccgatct cgaacaccag gctcgcgagg cgcagctcgg cttcgacgcg cttgcgttcg
     2101 gccacatcgc gcgcgatcgc gcaggcatag cgctcgccgc cggtttcgag cggcgtccac
     2161 gccacctcca gcgccagcga ccgcccgtcg gggccggtcg gggtcgcctc gtagctgtcc
     2221 ggctgcgtcg gcacgccggg cggtgcgaga ccaagctcgc cgaggctgcg accggtgatg
     2281 gccgcggccg ggcgcccgag cgtgcggcag gcggcgtcgt tggcatagac gacgcaaagc
     2341 cccgcatcgg cgaggaagat cgaatcggcg gccgcgtcca cgcagcgctg cgtcaggtac
     2401 agccggcgtg cgctcgcttc gagctcccgg cgccgggcgc gcagccgcgc ctgtacccag
     2461 cgcgcgtagg cgatcgagct gccgaggccg acactcagcg ccagcgccag cgcccacagc
     2521 gtggcgcgct cgcgcgcccg gacgccggct tcaagctcgc cgcgctggct ggcgagcgtg
     2581 acatcgaggt catcgagata cagaccggcg accagcgtcc agccccaggc gtcgaagggc
     2641 gtgaccaccg aaagcttcgt gcgcagcgga ccgcctgcgg gccggttcca gtcgtagcgc
     2701 acgacgccgc cgccggcacc ggcgcttgcg agcagctgtt cgagcaccgc acggcgcgtt
     2761 gcatcgaggg tggtgcggcc gttgctgcct tcgagatcgg gattcgccgg cgtgaccatc
     2821 atccggccgt cgcggtggaa cacggcgatg tagccgtcgt cctcgaagcg cagcgcgcgc
     2881 agccggtcga gtgcctcgcg ctgcagcatg cgcatcatcg tgtcgaggta ttcgccggtg
     2941 ccgagcagcc agcgatacgg ctcgaagcgc cgcacccagg tcagcttgtc gtccatgcgc
     3001 gtggcgtcgt tcgggggata ccagcgatag cgcagcaggc cggcaccttc gggctgcgtc
     3061 gcgacgcgga tcagctcgca catgatgcag acgccgttgt cgtcgcggat cggcagcagc
     3121 gagtggccct cgcgctccgg cgaggtcggc agcagcacgc agtcgccatc gagcgtatcg
     3181 atgaagtagt agccgcgacc gtcgaagaag cgcagcgggc gcagcgtctc gacgatcagc
     3241 ttctgcgttt cggccgtgcc gagcgggccg tcgaggaggc ggtgcatcga atagaccagt
     3301 tcgtacgcgg cctcggcctg ctcgcgcacg cgctcggcga gcagcgtctc ggcacgcgag
     3361 cgcagccagt cgagccagat ccgggtttcg tcgacacggc ggcgcagcag ggtttcctgc
     3421 tgtgcgagcg tttcacgttc gagctgatcg atccggcgtt cgacctcgcg gcggtagtcg
     3481 gcgatgaacc accagccgag cgcgagcacg agcgcggcga tcagtacaaa ggacacaccg
     3541 acctgcagcc agggcagata gtcctcatcg gtgagccggc gcaggggggt accgggcatt
     3601 gcgggtttca tggtgcgaaa ttttccacca tgccatcgac ctgacgcatg ggcgggctcg
     3661 cagggacaca caacttatct gtttgttaat gttctggcca ttccctttgc accggccgct
     3721 ccggcggggc tgtgccggca gggaaggcgg cggaagtccg gagtgtccgg aacaagcgca
     3781 cgggaaaggg atggagccga cggcgactgc ccggcaggca ggcgggatcc tgcgcgggag
     3841 cgccggaacc gggcgcgggc tccgggaggc cgcacacccg ctcgcggctg cgggagcgtg
     3901 ggggcgtcgc gatcagccgt acacggactg gaaggccggc tcgtgttccg gcagcggccg
     3961 gcgctggccc tcgccatcga cggcgacgta gacgatcacc gcctcggtga ccttcacgca
     4021 cagcgccggg cgctgcgcga agacctcgac gcgcacggtg atcgaggtcg tgccgatgcg
     4081 ctcgatgcgc gcgaagctgc tgaccagatc gccgacggtg accggctggt ggaactccat
     4141 cgaatgcacg gcgacggtcg cgacccgccc ctgcgcgtat tcgatcgcca gcaccgagcc
     4201 ggccaggtcc atctgcgcca tgatccagcc gccgaagatc tggccgtagg ggttggtgtc
     4261 ggccggcatc gcgacggtgc gcatggtcgg ctggcggttg cgcggaaact cggtaccgtt
     4321 ctcggggctg gacatgcggg gattacctcg gcgagattgc tgttttcacc ggcggcgcag
     4381 cccttgacgg cggtcatggc agcggcgcac gcccggcagt aagacggaaa accaacgata
     4441 acgcagcgcg acccgcacga cgggcgcgcg cacgacctgt aggaggggcg atggcgaagg
     4501 cgcgtaccgt ttgcatgtac ctcggtgagc agctcggccg ctacggcttc ggcgacgacc
     4561 acccgttcgg cgaggaccgc atccacgcct tcatggacga gatgcaccgt cgcgagctcg
     4621 actggcaggt gcgcatcctc gattcggtgg cctgcaccga gtccgaactg ctgctgttcc
     4681 atacgcccga atacgtcgcg cgcgtgcagg aaggctcgcg caccggcgag ggcatgctcg
     4741 attatggcga cacgccggcc tacgtcggcg tctacgacca cgtctcgcac gtggtcggcg
     4801 cggtgctcga tgccacgcac aagctgatga acgaccagat gcggcgctgc ttcgtgccga
     4861 tcgccggcct gcaccatgcg atgccggaca ccgcggccgg cttctgcgtg ttcaacgatg
     4921 tcggcgtcgc gatcaaggtg ctgcagcagc agtacggact ggagcgcatc gcctacgtcg
     4981 acatcgacgc gcaccacggc gacgggctgt tctatccgtt cgagaccgat ccgacggtga
     5041 tcttcgccga catccacgag gacggccgct acaacttccc gaacaccggc ttcccgcacg
     5101 agatcggcaa gggcgatgcg aagggccgca agctcaacgt gccgctgctg ccgctctccg
     5161 gcgatcagga attcaacatg atgtgggaga aggtcgaggg cttcgtcgac gactggaagc
     5221 cgcagttcat cttcgtcaac tgcggtgccg acggcctcga cggcgatccg ctcgcgcacc
     5281 tgcactactc gcagcaggcg cacaaccgcg cgatccgcag cctctgcagg attgccgagc
     5341 gccacagcga gggccgcatc ctcgccgtcg gcggcggcgg ctacgacctg cgcaacgtcg
     5401 gacgcgcgtg ggcgaccgtc gtcaacgcgc tgctggaatc gccgatgacc gtgaactgac
     5461 ggctgtcagc tccgcgttcc aggccgcaac cggcacgcag gctgccggtt gcggcctttt
     5521 tcattacact agcgcgcttt tccagccggc cggacgcccc ctgcgtccgg ccgcacgctt
     5581 tcaggggacg cggaaatgtt cggtaaagac atgcagatcg ccggctacga cgacgaactc
     5641 tgggcggcca tgcaggccga ggcccagcgt caggaagatc acatcgaact gatcgcctcg
     5701 gaaaactaca ccagcccgcg cgtcctgcag gcgcagggtt cggtgctgac caacaagtac
     5761 gcggaaggct atcccggcaa gcgttactac ggcggctgcg agtacgtcga tgtggccgag
     5821 agcctggcga tcgcgcgcgc gaagcagctc ttcggtgccg actacgccaa cgtccagccg
     5881 cattcgggct cgcaggccaa cgccgccgtc tacatggcgc tctgcgagcc gggcgacacc
     5941 gtgctcggca tgagcctcgc gcacggcggt cacctgaccc acggtgccag ggtcaacttc
     6001 tccggcaaga tctacaacgc cgtgcagtac ggcatcgacg acgagggccg catcgactac
     6061 gacgcgcttg cgcgcatcgc cgtcgagtgc aggccgaaga tgatcgtcgc cggcttctcg
     6121 gcgtattcgc aggtgctgga cttcgcgcgc ttccggcaga tcgccgatca ggtcggcgcg
     6181 tatctgttcg tcgacatggc gcacgtcgcc ggactggtcg ccgccggcct gtatccgaac
     6241 ccggtgccgt tcgccgacgt ggtcacgacc acgacgcaca agacgctgcg cggcccgcgc
     6301 ggcggcctga tcctcgcgaa ggccaacgag gcgatcgaga agaagctcaa ttcgctggtc
     6361 ttcccgggca cgcagggcgg tccgctgatg cacgtgatcg ccgccaaggc cgtcgccttc
     6421 aaggaggccc tggagccggc gttcaggagc tacgcgctgc aggtcgtgct caacgccaag
     6481 gccatggccg gcgtctgtct ggagcgcggc tacaaggtcg tctccggcgg caccgagaac
     6541 cacctgttcc tcgtcgatct ggtcagccac ggcatcaccg gcaaggccgc ggatgccgcg
     6601 ctcggtcagg cgcacatcac cgtcaacaag aactcggtgc cgaacgatcc gcagtcgccg
     6661 ttcgtgacct cgggcatccg catcggctcg ccggcgatca cgacccgcgg cttcaaggag
     6721 gccgaggtgc ggcaggtcgc gcactgggtc tgcgacgtgc tcgacgccgt cgacaagccc
     6781 gagctcgaag cgacggtcac gcgcgtgcgc ggcgaagtcg cggcgctctg cgcgcgcttc
     6841 ccggtctaca cgcactgaag ccgccccggc gcgcgccgcg atgcactgtc cgttctgcgg
     6901 cgctgccgac acccgcgtcg tcgactcgcg cctgtcgccc gaaggtgatc gcgtgcgccg
     6961 gcggcgcgaa tgcatcgcct gcggcgcgcg cttcacgacc ttcgagctcg ccgagctggt
     7021 gatgccgcgc gtcgtcaagc gcgacggccg gcgcgagccc ttcgccgggg acaagctgcg
     7081 cgccggcatg ctgcgcgcac tcgaaaagcg cccggtcggc gccgaggcga tcgacgcggc
     7141 gctggcgcgc atcacccggc gcgtgcgcgc gctcggtgag tccgaggtca gctcgaacca
     7201 gctcggcgaa tggatgatgg acgagttgcg ccggctcgat ccggtcgcct acgtgcgctt
     7261 cgcgtcggtg taccgcagct tcgaggacgt gaacgccttc cgcgaggaga tcgagcgcct
     7321 gcaggcaccg gcggacggac gcgaaccgtg agccggcctg ctgatcccgc caccgatgcc
     7381 cgccacatgg cacgcgcgct cgcgctcgcc gcgcgcggcc tgaacacgac cagcccgaac
     7441 ccgcgcgtcg gctgcgtgct ggtcgcgccc gatggcgagg tgatcggcga gggctggcac
     7501 gaacgcgccg gcgagccgca cgccgaggtc cacgcgctgc gcgcagccgg cggacgcgcg
     7561 cgcggcgcca cggcctacgt gacgctcgaa ccctgctcgc accacggccg cacgccgccc
     7621 tgcgccgatg cgctgatcga ggccggcgtc gcgcgcgtgg tcgcggcgat ggcggacccc
     7681 aatccgcagg tcgccggccg cggactcgcg cgcctgcgcg ccgccggcat cgccgtcgaa
     7741 tgcggcctgc tcgaagccga agcgcaggcg ctgaacgccg gcttcgtgcg gcgcatgacg
     7801 cgcgcgcggc cgtggctgcg gctgaagctc gccgcgagcc tcgatgcgcg cacggcgctc
     7861 gcgagcggcg aatcgcagtg gatcaccggc gaggcggcgc gcgccgacgt gcagcgcctg
     7921 cgcgcgcggg cctgcgccgt gctgaccggc agcggcacgg tgctcgccga tgatcccggc
     7981 ctcgacgtgc gcctgcccgg cacgcagcgc cagccgctgc gcgtcgtgct cgataccggg
     8041 ctgcgcacgc cgctcgccgc gcgcctgatc ggccggcccg ggcgcacgct gatcgtctgc
     8101 ggcccgcagg ccggcgtcgc cgaggcggcg gcgctggcgg cgtacggcgt cgacatcgag
     8161 cgcgtgccgg ttcagggcgg cgggctcgat ctcgacgcgg tgctcgcaag cctggcggcg
     8221 cgcggctgca acgaggttca tgccgagtgc ggcgcgcggc tggccggggc gctgctcgcc
     8281 gccgggcggg tcgatgagct cgtcgtctat ctggcaccgt tgctgcttgg cgatgccgcg
     8341 cgcggcctcg cgcggctgcc gggcctcgaa cgcatggagc agcgcctcga attcgactgg
     8401 accgacgtgc gcagcgtcgg tcgcgacctg cgcctgacgc tcgccccgcg cgggccggcg
     8461 gcgccggcgg tatcctgagc gcattcatcg ggtacgcggc catcgggccg tgcctctgtc
     8521 accgccggta ccggcgtccg gtaccggcct gcgagtcatc atgtcgctga acacgatcga
     8581 agaactgctc accgacctgc gtcaggggcg catggtgctg gtgatggacg acgaggaccg
     8641 cgagaacgaa ggcgatctgc tgatgatcgc ctcgctgacg cggcccgagg atgtcaactt
     8701 catggcccgc tacggtcgcg gcctgatctg cctgacgctg acccgcgagc gctgccagca
     8761 gctgcggctg ccgctgatgg tcaccaacaa cggcacgccg ttcgcgacca acttcacggt
     8821 ctcgatcgag gccgccgagg gtgtgaccac cggcatctcc gcgtacgacc gcgcccacac
     8881 gatccgtacc gccgtgcgcc ccgatgcgcg ccccgaggac ctcgtccagc ccggccacat
     8941 cttcccgctg atggcgcaga ccggcggcgt gctgacgcgc gccggccaca ccgaggccgg
     9001 ctgcgacctc gcgcgcctcg ccggctacga gccggcggcg gtgatctgcg aggtgctgaa
     9061 cgaggacggc acgatggcgc accggccgga gctcgaacgc ttcgccaccg agcacggcat
     9121 caagatgggc acgatcgcgg atctcatccg ctaccgtctc gccaacgagc cgaccgtcga
     9181 gcgcgtcgcc gaatgcccgc tgccgacgaa gcacggcgac ttccgtctct atgcctatca
     9241 ggacgtgctg acgaagcagc tgcattacgc gctggtcaag ggccggccgg ggccggccga
     9301 caccgcgctg gtgcgcgtac acgtgcagaa cacgcttggg gatctcttcg gtgcgcgcat
     9361 cgccgaatgc ggctggccgc tcgacgatgc gctggcccgc attgccgccg accccgcgcc
     9421 cggcgtcgtc atcgtgctcg cgcgtcagga ggagccgcgc gaaatggtgc ggcgcatccg
     9481 ccattatcag gtgcacggac cgcaggccgc caggccgccg gcgccggagt cggcgcagga
     9541 gctgcgcacc tacggcatcg gcgcgcagat cctgatcga
//
LOCUS       DAOQIE010000137         9502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3657_length_9502_cov_10.349211, whole genome shotgun sequence.
ACCESSION   DAOQIE010000137 DAOQIE010000000
VERSION     DAOQIE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3657_length_9502_cov_10.349211"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1222)
                     /locus_tag="PK144_09880"
     CDS             complement(8..1222)
                     /locus_tag="PK144_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF66077.1"
                     /translation="MADDTRDTPTPPPATGPAGSALHAAIAEYGVIALVLQGGGALGS
                     YQGGVAQGLLEAGVEPDWVAGISIGALNAAIIAGNPPERRLERLRAFWDAICAPQWLP
                     ASWAVQWPEAIVSRIDGWPRSLANAWDATRAILEGQPGFFRPRLPPYEVLLADTPSAA
                     SFYDTSPLRATLLEFADFERINHGPMRVSVGAVNVGTGNFTYFDNRQMRLAPEHFMAS
                     GALPPGFPAVEIDGEFYWDGGLVSNTPLSVILDEHPRPDALVFQVDLWSAGGTPPANV
                     AEVATRQKDIQYSSRTRMITELQRRDQQLRKLLLDLVKLVPGEHHDHPARREAVRLAC
                     ARRVNVQHLIYRDKPCESHYKDYQFGPATMREHWASGLDDIRKALSDPQRLVKPSADK
                     PFVTWDGDKKVV"
     gene            complement(1333..2115)
                     /locus_tag="PK144_09885"
     CDS             complement(1333..2115)
                     /locus_tag="PK144_09885"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNF66078.1"
                     /translation="MSLHGKVALITGAASGIGRNTAETLAAAGAAVAIADIRIEAAEQ
                     TAAEIRAATGSPAFGLAMDVTDEQAVGHGTQAVIEHLGGLDILVSNAGIQIVSPIEDF
                     AFSDWKRMLAIHLDGAFLTTRAALRHMYAHGGGTVIYMGSVHSHEASPLKSAYVAAKH
                     GLLGLARVLAKEGAAHNVRAHVVCPGFVRTPLVDKQIPEQAKELGISEEEVVKHVMLG
                     ETVDAQFTAVEDIGTMIRFLCEFPSTALTGQSFIVSHGWHMQ"
     gene            2337..4544
                     /locus_tag="PK144_09890"
     CDS             2337..4544
                     /locus_tag="PK144_09890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF66079.1"
                     /translation="MTPAHRALRRLILAFAVLTLIGVGAYWFVLLRDLDTQVETEAAH
                     LGEHAEYRALAAAESLDATLRQLDAVLTGARQDWIDEAGDFDALAQAALRALPPGLAI
                     QLFVIGTDGLLKWSSLGPAPANRLGDRDYFRRLAAAVDDPLEISEPVIGRLTGRWSIQ
                     FARRIERHGRFAGVVALSVSPERWARDIEKYAPSDGDSLALFRPDGAYLLRMPDPERH
                     YGQRVPADRPFLGGDPNQHVYTTAAVVDGLVRTYAWRRLDAGPIVVAGIGYEAALAPA
                     RAFAQRQILQAVLGTGLLLACAIVLRVLMRRADQATRALAESAERQRAVLSSMAEGVM
                     ILAPGGRIEYANAAACRELGLPSERELYHPADTVELDMLRPDGSPMPVEDYPSRRSAL
                     SGADFDNELLGRRAADGSVRWFTITTRALHRERSRAPYMVLMTFRDITALRSAEAEAR
                     LSQTVFEAAAEGILVTDGEHRIIAANPAFERLSGHRLDELCGQTPQLLTADLPQPVTW
                     AEVLDALATHDGWEGEGLIRRADARALAVLIHVTVLRDGAGRPWRHVALFSDITQRKR
                     REDEAWQRAHYDVLTGLPNRRLLDERLQRALALAEPGRHELAVLFVDLDRFKPVNDVH
                     GHALGDALLERVARRLEAAVREEDTVARYGGDEFVIAMLMPPGAPAAETVAVKLVEQL
                     ARPYRLDGRTLEIGASIGIAVQRAGDDAQTLLARADAAMYRAKQAHCGVWRDP"
     gene            5173..5871
                     /locus_tag="PK144_09895"
     CDS             5173..5871
                     /locus_tag="PK144_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNF66080.1"
                     /translation="MNKRVLIIVTSTPLIDGTGHPTGTWADDIAIPYYALREARCLVD
                     FASPLGGTAPIDPRSLCEVEGALPPSVVQFVADRELQQQLAGTRRVAEIDPLRFDALF
                     VPGGHGALWDLPGDAGVAQVVSSMLGYGQIVATLCHGAAALLGATGYGGRPLVAGRRV
                     TSITDAEESAVGLTAVLPVSLEQRLRELGAEFVHDELWRAYVTRDHALITGQNPQSAP
                     LIARHLVDALRLAG"
     gene            complement(5991..7541)
                     /gene="hutH"
                     /locus_tag="PK144_09900"
     CDS             complement(5991..7541)
                     /gene="hutH"
                     /locus_tag="PK144_09900"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNF66081.1"
                     /translation="MNASPILLRPGALDLALLRAVQEGEPPLCLEPGCRTAIDAAQAT
                     VTRVLAEGRTVYGVNTGFGLLAQTRIAAADLDTLQRSLVRSHAAGVGAPLPESTVRLV
                     LVLKIASLARGHSGVRAELVEALLALYTHGIRPLIPSRGSVGASGDLAPLAHLAAVLI
                     GEGEATYRGVRMSGAAALAAAGLAPLALAPKEGLALLNGTQVSTALALEGLFAAENLL
                     VAALVTGSLSVEAALGSRRPFDARIHAVRGHAAQIDIAAACRALLDHSEIERSHEHCG
                     KVQDPYSLRCQPQVMGACLEQIRHAAAVLLTEANAVSDNPLVFADDDDILSGGNFHAE
                     PVAFAADGLALALAETGALAERRIAMLIDANLSNGLPAFLVAHGGLNSGFMAAQVTAA
                     ALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLAELAANTAGIVAIEWLAAAQ
                     GVDLRAPHRSSAALRTALALLRAAVPFYERDRCFAPDIARATALIEGGQLRAAVRDAL
                     PAGLLPSG"
     gene            7731..9431
                     /locus_tag="PK144_09905"
     CDS             7731..9431
                     /locus_tag="PK144_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66082.1"
                     /translation="MNEPPVDGARGISALGQRLALTGASDGLWEWNPLTKALYVSPRL
                     LAILGYEQDLLPDTHAWLALIHPEDRDGYNHAVATHLQGRTPYFEHEYRVRAADGAWR
                     WVSARGIAARDAGGRAYCMAGSVADITARKVMEAGMRHLAHHDALTGLPNRTLLEDRF
                     AQALSGARRSGGGLALLFLDLDRFKDVNDAWGHPVGDALLRAVAERLKTSVRAVDTVA
                     RHGGDEFIVVLPATGTPAAAALVARKILAALDAPVEFDAYRLYVRASLGIALYPRDGT
                     DFATLLKCADTALYRAKSAGRGCLRFYTAQMDAELRGRLAIEQGLRTVLSEGGLVLHY
                     QPVFAAGTPGRPLAAEALLRWQRPDGVCVPPAEFIPIAEETGLIVPIGDWVLAEACRQ
                     AQRWHAWIAAGAAIAVNLAVPQLRQPRLPERIAQLLADHGLPPAALEIELTESMLMQR
                     GSASEHVLHRLHAMGLRIAIDDFGTGYSSLAYLRRLPIDTLKIDRSFVAELDTAGGGA
                     IPGAVIALAGQLGMQTTAEGVETLAQAQWLHARGCDRLQGYLHGRPVPADEFERCHLR
                     "
BASE COUNT         1289 a         3420 c         3432 g         1361 t
ORIGIN      
        1 cggcgcttca gacgaccttt ttgtccccgt cccaggtcac gaagggtttg tccgcgctcg
       61 gcttcacgag gcgctgcggg tcgctcaggg ccttgcggat gtcgtcgagg ccgctggccc
      121 agtgttcgcg catcgtcgcc gggccgaact ggtagtcctt gtaatggctc tcgcagggct
      181 tgtcgcggta gatcaggtgc tggacgttga cgcgccgcgc gcaggccagg cgcacggcct
      241 cgcgccgcgc cggatggtcg tggtgctcgc cgggaacgag cttcaccaga tcgagcagca
      301 gcttgcgcag ctgctgatcg cggcgctgca gctcggtgat catgcgcgtg cggcttgaat
      361 actggatgtc cttctgccgg gtcgcgacct cggcgacgtt ggccggcggc gtgccgccgg
      421 cgctccagag atcgacctgg aacaccagcg cgtccgggcg cgggtgttcg tcgaggatca
      481 ccgacagcgg cgtgttcgag accaggccgc cgtcccagta gaactcgccg tcgatctcga
      541 ccgccggaaa gcccggcggc agcgcgcccg aggccatgaa gtgctcgggc gcgaggcgca
      601 tctggcggtt gtcgaagtac gtgaagttgc cggtgccgac gttcacggcg ccgaccgaca
      661 cgcgcatcgg cccgtgattg atgcgctcga agtcggcgaa ttcgagcagc gtcgcgcgca
      721 gcggcgaggt gtcgtagaag ctcgccgccg agggcgtgtc ggcgagcagc acctcgtagg
      781 gcggcagccg cggccggaag aagccgggct ggccttcgag gatcgcgcgc gttgcgtccc
      841 aggcgttggc gaggctgcgc ggccagccgt cgatgcggct gacgatggcc tcgggccact
      901 gcacggccca ggaggccggc agccactgcg gcgcgcagat cgcatcccag aacgcgcgca
      961 ggcgttcgag ccgccgctcc ggcgggttgc cggcgatgat cgcggcgttc agcgcgccga
     1021 tcgagatgcc ggccacccag tccggttcga cgccggcctc cagcaggccc tgcgcgacgc
     1081 cgccctgata cgaaccgagc gcgccgccgc cctgcagcac cagggcgatg acgccgtact
     1141 cggcgatggc ggcgtgcagc gcactgccgg ccgggccggt cgccggcggc ggggtggggg
     1201 tgtcgcgggt atcgtcggcc atgcggggca gtctcgtgcg agcggggaat ccggcggccc
     1261 gggggtctcg tgcccaccgt agcgcaggcc ggcgccggac gcgccggcgg gtctgcgtgc
     1321 cggctgcggg gtctactgca tgtgccagcc gtggctgacg atgaagctct ggccggtcag
     1381 cgccgtgctc gggaactcgc acaggaagcg gatcatcgtg ccgatgtcct cgaccgcggt
     1441 gaactgcgca tcgacggtct cgccgagcat cacgtgcttc acgacttcct cctcgctgat
     1501 gccgagttcc ttcgcctgct ccggaatctg cttgtcgacc agcggcgtgc gcacgaagcc
     1561 cgggcagacg acgtgggcgc gcacgttgtg ggctgcgcct tccttcgcca gcacgcgcgc
     1621 cagcccgagc aggccgtgct tggcggcgac gtaggccgac ttcagcggcg aggcctcgtg
     1681 cgaatgcacc gagcccatgt agatcaccgt gccgccgccg tgcgcgtaca tgtgccgcag
     1741 ggcggcgcgc gtggtcagga aggccccgtc gagatggatc gccagcatcc tcttccagtc
     1801 ggagaacgcg aagtcctcga tcgggctgac gatctggatg ccggcgttcg agaccaggat
     1861 gtcgaggccg ccgaggtgct cgatgacggc ctgcgtgccg tgcccgaccg cctgctcgtc
     1921 ggtcacgtcc atcgccagac cgaaggcggg gctgccggtg gcggcccgga tttcggcggc
     1981 ggtctgttcg gcggcttcga tgcggatgtc ggcgatcgcg accgcggcgc cggcagcggc
     2041 cagggtttcg gcggtgttgc ggccgatgcc gctcgcagca ccggtgatca gggcgacttt
     2101 accgtggagg ctcatggggg attcctcttg cgggtggacg aaagcgcgac cggccgggtg
     2161 cccggattca attgcaagac ccggaccggc aagacccgga ccgcaaaccg cggcgatctt
     2221 cgtgatgcgg ccatgccgcc cggaccccgg atcgaggctg cagccgcggc cgtgcctgca
     2281 ggcaccgacc gccggatacc gagaccgttg tcttaagcct ggcttacgct aggcacatga
     2341 ctcctgcgca ccgcgccctg cgccgcctga tccttgcctt tgccgtgctg accctgatcg
     2401 gtgtcggtgc ctactggttt gtgctgctgc gcgatctcga tacccaggtc gagaccgagg
     2461 cggcgcacct cggcgagcac gccgaatacc gggcgctggc cgcggccgag agcctcgacg
     2521 cgacgctgcg ccagctcgat gccgtgctca ccggtgcgcg gcaggactgg atcgacgagg
     2581 ccggcgactt cgatgcgctc gcacaggcgg cgctgcgcgc gctgccgccg gggcttgcga
     2641 tccagctgtt cgtgatcggc accgacggcc tgctcaagtg gtcgagcctg ggtccggcgc
     2701 cggcgaaccg gctcggggat cgtgactact tccgccgcct cgccgcagcc gtggatgacc
     2761 cgctggagat cagcgagccg gtgatcggcc ggctgaccgg gcgctggagc atccagttcg
     2821 cgcggcgcat cgagcgccac ggccgcttcg ccggcgtcgt ggcgctgtcg gtgtcgcccg
     2881 agcgctgggc acgcgacatc gagaagtacg ccccctcgga cggcgattca ctggcgctgt
     2941 tccggcccga cggggcctat ctgctgcgca tgcccgaccc cgagcgccac tacggccagc
     3001 gcgtgccggc cgaccggccc tttctcggcg gcgacccgaa ccagcacgtc tacacgaccg
     3061 ctgccgtcgt cgacggtctg gtgcgcacct acgcgtggcg gcgcctcgat gccgggccga
     3121 tcgtggttgc cggcatcggc tacgaggccg cgctcgcgcc ggcgcgcgcc ttcgcgcagc
     3181 gccagatcct gcaggccgtg ctcggcaccg gcctgctgct cgcctgcgcg atcgtgctgc
     3241 gcgtgctgat gcgccgcgcc gatcaggcca cgcgcgcgct cgccgaaagc gccgagcgcc
     3301 agcgcgccgt gctgtcgtcg atggccgagg gcgtcatgat cctcgcgccg ggcgggcgca
     3361 tcgagtacgc caacgccgct gcctgccgcg aactcgggct gccgagcgaa cgcgagctgt
     3421 accacccggc cgataccgtc gagctcgaca tgctgcgccc ggacggcagc ccgatgccgg
     3481 tcgaggacta tccgagccgc cgcagtgcgc tcagcggcgc cgacttcgac aacgaactgc
     3541 tcggccggcg cgccgccgac ggcagcgtgc gctggttcac gatcacgacg cgcgcgctgc
     3601 accgcgagcg cagccgcgcg ccgtacatgg tgctgatgac cttccgcgac atcacggcgc
     3661 tgcgcagcgc cgaggccgag gcgcggctga gccagaccgt gttcgaggcc gcggccgaag
     3721 gcatcctcgt caccgacggc gagcaccgca tcatcgccgc caacccggcc ttcgagcgcc
     3781 tgagcggcca ccggctcgac gagctgtgcg gacagacgcc gcagctgctc accgctgatc
     3841 tgccgcagcc ggtgacctgg gccgaggtcc tcgatgcgct ggccacgcat gacggctggg
     3901 aaggcgaggg cctgatccgg cgtgcggacg cccgggcgct ggcggtgctg atccacgtca
     3961 ccgtgctgcg cgacggcgcc ggccggccgt ggcggcatgt ggcgctgttc agcgacatca
     4021 cgcagcgcaa gcgccgcgag gacgaggcct ggcagcgcgc gcactacgac gtgctgaccg
     4081 gcctgccgaa ccgccgcctg ctcgacgagc gcctgcagcg cgcgctggcg ctggccgaac
     4141 ccggccggca cgagctcgcc gtgctgttcg tcgatctcga ccgcttcaag ccggtcaacg
     4201 atgtccacgg ccacgcgctc ggcgacgcgc tgctcgaacg cgtcgcgcgc cggctcgaag
     4261 ccgcggtgcg cgaggaggac acggtcgcgc gctacggcgg cgacgagttc gtgatcgcga
     4321 tgctgatgcc gcccggcgcg cccgccgccg aaaccgtcgc cgtcaagctg gtcgagcagc
     4381 tcgcgcgccc gtaccggctc gacggccgca cgctggagat cggcgccagc atcggcatcg
     4441 ccgtgcagcg cgccggcgac gacgcgcaga cgctgctcgc gcgcgccgac gcggcgatgt
     4501 accgcgccaa gcaggcgcac tgcggcgtct ggcgcgaccc atagcccccg ggcgcgtgac
     4561 cagtgcttgc aggacgcacg gattcggcaa gcttcccgga agcccgccgc cgcctgtgta
     4621 aggctggaag ccgcaagcgc aaggcttttt cccttgcgct ccgcgctcga agccgcaagc
     4681 gcaaggcttt ttcccttgca cctcgcgctc gaagccgcaa gcgcaaggct ttttcccttg
     4741 cgctccgcgc tcgaagccgc aagcgcaagg ctttttccct tgcgcctcgc gctcgaagcc
     4801 gtgcacgcaa ggctttttcc cttgcgctcc gcgctcgaag ccttgcacgc agggcttttt
     4861 cccttgcact tcgcgctcga agccttgcac gcgacgcgaa aagcgtcgcg accggcgccc
     4921 gcagcctcag cgccgccgcc ggcagccggt cccggcccgc ggtctggcgc gcgcccgctc
     4981 ctgcgggcgc cgggttccgc acggtcgccg gagggtgtcc ccgtgcaggc cgctcccggt
     5041 ggtccctgaa aaacaaggat cgtcagacat ttgcgtgccg tgatcttgcc ggatgctcat
     5101 gtgcgcggct gaaccgctcc cccccggcag ggtcggtccg cacacgggct gagccgtgga
     5161 caggagcgga tcatgaacaa gcgtgtgctg atcatcgtca cgtccacgcc gctgatcgac
     5221 ggcaccggcc acccgaccgg cacctgggcc gatgacatcg cgattccgta ttacgcgctg
     5281 cgcgaagccc gctgcctcgt ggatttcgcc tcgccgctcg gcggaacggc gccgatcgat
     5341 ccgcgcagcc tgtgcgaggt cgagggcgcg ctgccgccct cggtcgtgca gttcgtcgcg
     5401 gaccgcgagc tgcagcagca gcttgccggg acccggcgcg tcgccgagat cgatccgctg
     5461 cgcttcgatg cgctgttcgt gcccggcggc cacggcgcgc tgtgggatct gccgggcgat
     5521 gccggcgtcg cgcaggtggt cagctcgatg ctcggctacg gccagatcgt cgcgacgctc
     5581 tgccacggcg ccgcggcgct gctcggcgcc accggctacg gcggccggcc gctggtcgcc
     5641 ggccggcgcg tgacctcgat caccgatgcc gaggaaagcg cggtcggcct gaccgcggtg
     5701 ctgccggtgt cgctggaaca gcgcctgcgc gagctcggcg ccgaattcgt gcacgacgag
     5761 ctgtggcgcg cgtacgtgac gcgcgaccac gcgctgatca ccggccagaa cccgcagtcg
     5821 gcaccgctga tcgcgcgcca tctggtcgac gcgctgcgcc tcgccggctg aacgcccctg
     5881 ccggcctcat gccagtcaca gctgaacacc cgatcgtttc agcccctccc ccctcgcggg
     5941 ggaggggttg gggagagggg gatcaacagc gcatgcacgt gtgatcggta tcagccgctc
     6001 ggcagcagtc cggccggcag tgcgtcacgc accgcggcgc gcaactgccc gccctcgatc
     6061 agcgccgtgg cgcgggcgat gtcgggcgcg aagcagcggt cgcgctcgta gaacggcacc
     6121 gcggcgcgca gcagcgccag cgccgtgcgc agcgctgccg agctgcggtg cggtgcgcgc
     6181 aagtccacgc cctgcgccgc cgccagccat tcgatcgcga cgatgccggc ggtgttggcc
     6241 gccagctccg cgagccggcg cgccgcgaag gtcgccatcg acacgtgatc ctcctgattg
     6301 gcgctggtcg gcaggctgtc gaccgaggcc ggatgcgcga gcgacttgtt ctcggaggcg
     6361 agtgccgcgg ccgtgacctg cgcggccatg aagccggagt tgaggccgcc gtgcgcgacc
     6421 aggaacgccg gcaggccgtt gctcaggttg gcgtcgatca gcatcgcgat gcgccgctcg
     6481 gcgagcgcgc cggtttcggc gagcgcgagc gccagaccat cggccgcgaa cgccaccggc
     6541 tcggcgtgga agttgccgcc ggagaggatg tcgtcgtcgt cggcgaacac cagcgggttg
     6601 tcggacacgg cgttggcctc ggtcagcagc accgcggcgg cgtggcggat ctgttcgagg
     6661 caggcgccca tgacctgcgg ctggcagcgc agcgagtacg ggtcctgcac cttgccgcag
     6721 tgctcgtgcg agcgttcgat ctcgctgtga tcgagcagcg cgcggcaggc ggcggcgatg
     6781 tcgatctgcg ccgcatggcc gcgcacggcg tggatgcgcg catcgaaggg ccggcggctg
     6841 ccgagcgcgg cctcgaccga caggctgccg gtgacgaggg ccgcgacgag caggttctcg
     6901 gccgcgaaca ggccctcgag cgcgagcgcc gtcgatacct gcgtgccgtt gagcagcgcc
     6961 agcccctcct tcggcgccag cgcgagcggc gccagccccg ccgccgccag cgccgcggca
     7021 ccgctcatgc gcacgccccg gtacgtcgcc tcgccctcgc cgatcagcac cgccgccaga
     7081 tgcgcgagcg gcgccagatc gccggacgcg ccgaccgagc cgcgcgacgg gatcagcggc
     7141 cggatgccgt gcgtgtacag cgcgagcagc gcctcgacga gttcggcgcg cacgccggaa
     7201 tggccgcgcg cgaggctcgc gatcttcagc accagcacca ggcgcacggt cgattccggc
     7261 agcggcgccc cgacgccggc ggcgtgcgag cgcaccaggc tgcgctgcag cgtgtcgaga
     7321 tcggccgctg cgatgcgcgt ctgcgcgagc aggccgaagc cggtgttgac gccgtagacc
     7381 gtgcggcctt cggcgagcac gcgcgtgacc gtcgcctggg cagcgtcgat cgccgtccgg
     7441 cagccgggct caagacagag cggcggctcg ccttcctgca cggcgcgcag cagcgccagg
     7501 tcgagcgcgc cggggcgcag caggatcggg gaagcgttca tcgcgcaagg ctccggccgc
     7561 ggccgacgcg actgcccgat ggcgcagccc gcgtgccgcg gcaccgcgaa atagtggcag
     7621 atgccggtgc cgggcacgaa cccgtgccgg acggcgccgg cgatgcggcc tcgggcagat
     7681 gcgggggctg cacccgtgag gctgccgcgg cccggcgata tgctgcggcg atgaacgaac
     7741 caccggtgga cggcgcgcgc ggcatcagcg ccctgggaca gcgtctggcg ctgacgggcg
     7801 cgagcgacgg gctgtgggaa tggaatccgc tgaccaaggc cttgtacgtg tcgccgcgcc
     7861 tgctcgcgat cctcggctac gagcaggatc tgctgcccga cacgcatgcg tggctggccc
     7921 tgatccaccc cgaggatcgc gacggctaca accatgccgt cgccacgcat ctgcaggggc
     7981 gcacgccgta tttcgagcac gagtaccgtg tgcgcgccgc cgacggtgcc tggcgctggg
     8041 tgtcggcgcg cggcatcgcg gcgcgcgacg ccggcggtcg cgcgtactgc atggccggct
     8101 cggtcgccga catcaccgcg cgcaaggtga tggaggcggg gatgcgccac ctcgcgcatc
     8161 acgatgcact gaccggcctg cccaaccgca cgctgctcga agaccgcttc gcgcaggcgc
     8221 tgtccggtgc ccggcgctcg gggggcgggc tggcgctgct gtttctcgat ctcgaccgct
     8281 tcaaggatgt caacgatgcc tggggccacc cggtcggcga tgcgctgctg cgcgcggtgg
     8341 ccgagcgtct gaagacgagc gtgcgcgccg tcgatacggt cgcgcgtcac ggcggcgacg
     8401 agttcatcgt cgtgctgcca gcaaccggaa ccccggccgc tgcggccctc gtcgcgcgca
     8461 agatcctcgc ggcgctcgat gccccggtcg agttcgacgc gtaccggctg tacgtgcgcg
     8521 caagcctcgg catcgcgctg tacccgcgcg atggcacgga cttcgccacg ctgctcaagt
     8581 gcgccgacac ggcgctgtac cgcgccaaga gcgccggccg cggctgtctg cgcttctaca
     8641 cggcgcagat ggatgccgag ctgcgcggcc ggctggcgat cgagcagggc ctgcgcacgg
     8701 tgctgagcga gggcgggctg gtgctgcact accagccggt cttcgccgcc ggtacgcccg
     8761 ggcggccgct cgccgccgag gcgctgctgc gctggcagcg cccggacggt gtctgcgtgc
     8821 cgccggccga attcatcccg atcgccgagg aaaccgggct gatcgtgccg atcggcgact
     8881 gggtgctcgc cgaggcctgc cgtcaggcgc agcgctggca tgcgtggatc gccgccggcg
     8941 ctgcgatcgc cgtcaatctg gccgtgccgc agctgcgtca gccgcggctg cccgagcgca
     9001 tcgcccagct gctggcagac cacgggctgc cgccggcagc gctggagatc gagctgactg
     9061 aatcgatgct gatgcaacgt ggcagcgcgt ccgagcacgt gctgcaccgc ctgcatgcaa
     9121 tgggcctgcg catcgccatc gacgacttcg gcaccggcta ttcctcgctc gcctacctgc
     9181 gccggctgcc gatcgacacc ctgaagatcg accgcagttt cgtcgccgaa ctcgacacgg
     9241 ccggcggcgg cgccatcccc ggcgcggtca tcgcgctcgc cggccagctc ggcatgcaga
     9301 ccacggccga aggcgtggaa accctcgcgc aggcgcagtg gctgcatgcg cgcggctgcg
     9361 accggctgca gggttacctg cacggccgcc cggtgccggc ggacgaattc gaacgctgcc
     9421 atctccgctg agcgcgggcg gcggcttcag cccgcgtgcc acagcaccgc gaaatagtgg
     9481 cagacgctgc cgccgagcac ga
//
LOCUS       DAOQIE010000138         9424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3697_length_9424_cov_8.296510, whole genome shotgun sequence.
ACCESSION   DAOQIE010000138 DAOQIE010000000
VERSION     DAOQIE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_9424_cov_8.296510"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /gene="rpoB"
                     /locus_tag="PK144_09910"
     CDS             <1..1817
                     /gene="rpoB"
                     /locus_tag="PK144_09910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNF66083.1"
                     /translation="YNLTKYTRSNQNTCINQRPLVNLGDAVEEGDVLADGPSTDLGEL
                     ALGQNMLVAFMPWNGYNFEDSILISERVVKEDRFTTIHIEELTCVARDTKLGPEEITA
                     DIPNVGEAALAKLDEAGIVYIGAEVAAGDILVGKVTPKGETQLTPEEKLLRAIFGEKA
                     SDVKDTSLRVPTGMDGTVIDVRVFTRDGVEKDSRAKSIEAAELARVKKDLNDQLRILE
                     EDTFQRVERMLIGKTAEGGPDGLAAGAKIAKSYLQKLPRDRWFEIRLRDDEANEQLEA
                     FAEKRKQQRKEFDKKFEDKRAKLIQGDDLAPGVLKMVKVYLAVKRRVQPGDKMAGRHG
                     NKGVVSMIVPEEDMPYMEDGTPVDVCLNPLGVPSRMNIGQILETHLGWAAKGAGLRIG
                     RMLQAKAAVEDLRALLHDIYNTTGQLDQVDLAQFSDAEVVRLCENLRAGVPTATPVFD
                     GCSEDELKALLRLAGLAETGQTRLIDGRTGDPFDREVTVGYMYMLKLNHLVDDKMHAR
                     STGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKV
                     YKNIVDGDHRMEAGMPESFNVLVKEIRSLGINIELDRD"
     gene            1896..6113
                     /gene="rpoC"
                     /locus_tag="PK144_09915"
     CDS             1896..6113
                     /gene="rpoC"
                     /locus_tag="PK144_09915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNF66084.1"
                     /translation="MKDLLNYLPKTETLDDFDAIRIGLASPDMIRTWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVVCEKCGVEVTLAKVRRERM
                     GHIELAAPVAHIWFLKSLPSRIGLLLDMTLRDIERVLYFESFVVINPGLTPMERGQLL
                     SDENYLDAIEQYGDDFDARMGAEAVHELLKSMDLKELAGRLREELVDCTSETKLKRIS
                     KRLKLMESFIESGNHPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELSAPDIIVRNEKRMLQEAVDSLLDNGRRGRAITGTNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKKMALELFKPFIFSKLIRRGVAT
                     TIKAAKKMVEREQPEVWDVLEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAFNADFDGDQMAVHVPLSIEAQLEARALMMSTNNILSPANGDPIIVPSQDVVL
                     GLYYMTRERINARGEGMRFTDVKEVHRAYETRQAELQARIEVRIVETLIHADGSRERV
                     RRRVSTTVGRALLFEIFPEGLPFELANQVLKKKTISGLINQCYRRLGLKDTVIFADQL
                     MYTGFHYAMRSGSSVGFTDMRIPDEKAGILARAESEVKQIEEQYASGLVTNGERYNKV
                     VDIWSRTNDEVTKAMMRGLGKETVVNREGQTVEQDSFNSVFMMADSGARGSQAQMRQL
                     AGMRGLMAKPDGSIIETPITANFREGLDVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEEDCGTREGLLMTPIVEGGDVVEPLRERVLGRVVAEDVCRPGSGEV
                     VAPAGTLLDEHWVDVLESAGIDQVLVRSPITCETRYGVCSSCYGRDLARGHIVNIGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAVSSVQVKARGRIRLHNLKTVTNKDGN
                     LVAVSRSGEVTVMDDNGRERERYKIPYGAVLTVKEGDSVEAGQIAANWDPHTHPVVTE
                     VDGRVRFLDFEEGSTVTRKTDEITGLSSLEVLDPKARGSLGKDKRPTVRLVDAAEQPL
                     KLAGTDLPAQYPLPPGAIVNLTDGAEVRVGDIVARIPQESSKTRDITGGLPRVADLFE
                     ARKPKEPAILAEISGTVSFGKDTKGKQRLVLTDKDGGTHEELIPKWRHVNVFEGEQVE
                     RGEVIADGEPNPMDILRLRGVRDLAAYLVKEIQDVYRLQGVKINDKHIEVIIRQMMRK
                     VEISEPNNARFIKGEQVERTRVLEENDRVRGDNGVPAHWHPLLLGITKASLATESFIS
                     AASFQETTRVLTEAAVRGSRDDLRGLKENVIVGRLIPAGTGLAYHADRRGGRTTPRPG
                     SSFGTEFSTAEFGSVDYSAGGESHAEE"
     gene            6315..6689
                     /gene="rpsL"
                     /locus_tag="PK144_09920"
     CDS             6315..6689
                     /gene="rpsL"
                     /locus_tag="PK144_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006083605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNF66085.1"
                     /translation="MATINQLVRKPRSSKPEKSAVPALNGCPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTIRGCL
                     DTSGVQKRRQGRSKYGAKRPKS"
     gene            6744..7214
                     /gene="rpsG"
                     /locus_tag="PK144_09925"
     CDS             6744..7214
                     /gene="rpsG"
                     /locus_tag="PK144_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNF66086.1"
                     /translation="MPRRREVPKRTILPDPKFGNEMLAKFMNMVMERGKKSVAEKIIY
                     GALDAITEKGKDDAMSVVEQALDNVRPAVEVKSRRVGGATYQVPVEVRQVRRNTLAMR
                     WIIDAARKRGEKSMARRLAGELLDASESRGAAVKKREDTHRMADANKAFAHYRW"
     gene            7232..9325
                     /gene="fusA"
                     /locus_tag="PK144_09930"
     CDS             7232..9325
                     /gene="fusA"
                     /locus_tag="PK144_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF66087.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMAQQFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYGVPRIAFVNKMDRAGANFLRVVQQMKD
                     RLKANPVPLQIAIGAEDAFEGIVDLITMKAIYWNDDTQGTSFELRDVPADLADLCAEW
                     REKLIEAAAEANEELMDKYLEGEELSEDEIHAAIRSRTIAGEIVPALCGSAFKNKGVQ
                     AMLDAVVRYLPAPVDIPAVTGTLDDGSEASREPADAAPFSALAFKIATDPFVGTLTFF
                     RVYSGVLSSGDSVLNSVKGKKERIGRIVQMHANSREEIKEVLAGDIAAAVGLKDVTTG
                     DTLCDPEKPIVLERMEFPEPVISVAVEPKTKSDQEKMGLALNRLAQEDPSFRVHTDEE
                     SGQTIISGMGELHLEIIVDRMKREFKVEANVGAPQVAYRETIRASVEQEGKFVRQSGG
                     RGQYGHVWLKIEPQEAGKGYEFVNGIVGGVVPREYVPAVDKGIQEAMADGVLAGFPVV
                     DVKVTLFDGSYHDVDSNEMAFKIAGSMGFKEGFRKARPALLEPIMQVEVETPEDYMGD
                     VIGDLNRRRGLVQGMDDSPSGKIVKAEVPLAEMFGYATSLRSATQGRATYSMEFAKYN
                     EAPANIAEAVIKKAS"
BASE COUNT         1775 a         3086 c         3158 g         1405 t
ORIGIN      
        1 tctacaacct gaccaagtac acgcgctcga accagaacac ctgcatcaac cagcggccgc
       61 tggtgaatct cggtgatgcg gtcgaggagg gtgacgtgct ggccgacggt ccctcgaccg
      121 acctcggcga gctggcgctc ggccagaaca tgctggtcgc gttcatgccg tggaacgggt
      181 acaacttcga ggactcgatc ctgatctcgg agcgcgtcgt caaggaggac cgcttcacga
      241 cgatccacat cgaggagctg acctgcgtcg cgcgcgacac caagctcggg cccgaggaaa
      301 tcaccgccga catcccgaac gtcggcgagg cggcgctcgc gaagctcgat gaggccggca
      361 tcgtctacat cggtgccgag gtcgcggccg gggacatcct ggtcggcaag gtcacgccga
      421 agggcgagac ccagctcacg cccgaggaaa agctcctgcg cgcgatcttc ggcgagaagg
      481 cctcggacgt gaaggacacc tcgctgcgcg tgccgaccgg catggacggc accgtcatcg
      541 acgtgcgcgt gttcacgcgc gacggcgtcg agaaggacag ccgtgccaag tcgatcgagg
      601 cagccgagct tgcccgcgtc aagaaggacc tcaacgacca gctgcgcatc ctggaggagg
      661 acaccttcca gcgcgtcgag cgcatgctga tcggcaagac cgccgagggc ggcccggatg
      721 gcctcgcggc cggcgccaag atcgccaagt cctacctgca gaagctgccg cgtgaccgct
      781 ggttcgagat ccgcctgcgc gacgacgaag ccaacgaaca gctggaggcc ttcgccgaga
      841 agcgcaagca gcagcgcaag gagttcgaca agaagttcga ggacaagcgc gcgaagctga
      901 tccagggcga cgacctcgcg ccgggcgtgc tgaagatggt caaggtctac ctggccgtga
      961 agcgtcgcgt gcagcccggc gacaagatgg ccggccgcca cggcaacaag ggtgtcgtgt
     1021 cgatgatcgt gcccgaggag gacatgccgt acatggagga cggcacgccg gtcgacgtgt
     1081 gcctgaaccc gctcggcgtg ccttcgcgca tgaacatcgg ccagatcctc gaaacccacc
     1141 tcggctgggc ggcgaagggc gcgggcctgc gcatcggtcg catgctgcag gcgaaggcgg
     1201 cggtcgagga cctgcgcgcg ctgctgcacg acatctacaa caccaccggc cagctcgatc
     1261 aggtcgatct cgcgcagttc tcggacgccg aggtcgtgcg cctctgcgag aacctgcgcg
     1321 ccggcgtgcc gaccgcgaca ccggtgttcg acggctgctc cgaggacgag ctgaaggcgc
     1381 tgctgcgtct ggccggcctg gccgagaccg gccagacccg gctgatcgac ggtcgcaccg
     1441 gtgacccgtt cgatcgcgag gtcacggtcg gctacatgta catgctcaag ctcaaccacc
     1501 tcgtcgacga caagatgcac gcgcgctcga ccggtccgta ctcgctggtc acgcagcagc
     1561 cgctcggcgg caaggcgcag ttcggcggcc agcgcttcgg cgagatggag gtctgggcgc
     1621 tcgaggccta cggcgccgcg tacacgctgc aggagatgct gacggtcaag tccgacgacg
     1681 tgaacgggcg caccaaggtc tacaagaaca tcgtcgacgg cgaccaccgc atggaggccg
     1741 gcatgcccga gtccttcaac gtgctggtca aggaaatccg ctcgctcggc atcaacatcg
     1801 agctggatcg ggactgacga gagccccgcg ccgcccccgg ttcagttccg gggcggcgcg
     1861 gccggcggct tgcgaacaga cagagcggtc acgagatgaa agatctgctg aattacctcc
     1921 cgaagacgga aaccctcgac gacttcgatg cgatccgcat cggtctggcc tcgccggaca
     1981 tgatccggac ctggtcgtac ggcgaggtca agaagcccga gacgatcaac taccgcacgt
     2041 tcaagccgga acgcgacggc ctgttctgcg ccaagatctt cggaccggtg aaggactacg
     2101 agtgcctctg cggcaagtac aagcgcctga agcaccgcgg cgtggtctgc gagaagtgcg
     2161 gcgtcgaggt gacgctcgcc aaggtgcgcc gcgagcgcat gggccacatc gagctggccg
     2221 cgccggtcgc gcacatctgg ttcctgaagt cgctgccctc gcgcatcggc ctgctgctcg
     2281 acatgacgct gcgcgacatc gagcgcgtgc tgtacttcga atccttcgtc gtcatcaatc
     2341 cgggcctgac gccgatggag cgcggccagc tgctgagcga cgagaattac ctcgacgcga
     2401 tcgagcagta cggcgatgac ttcgacgcgc gcatgggcgc cgaggccgtg cacgagctgc
     2461 tgaagtcgat ggacctcaag gagctcgccg ggcgcctgcg cgaggaactg gtcgactgca
     2521 cctccgagac caagctcaag cgcatctcca agcgcctgaa gctgatggag tccttcatcg
     2581 agtccggcaa ccatcccgag tggatggtca tgaccgtgct gccggtgctg ccgcccgacc
     2641 tgcggccgct ggtgccgctc gacggcggac gcttcgcgac ctcggatctc aatgacctgt
     2701 accggcgcgt catcaaccgc aacaaccgcc tgaagcgcct gctggagctg tcggcgcccg
     2761 acatcatcgt gcgcaacgag aagcgcatgc tgcaggaggc cgtcgactcg ctgctcgaca
     2821 acggccgccg cggccgcgcg atcaccggca ccaacaagcg gccgctgaag tcgctcgccg
     2881 acatgatcaa gggcaagcag gggcgcttcc gccagaacct gctcggcaag cgcgtcgact
     2941 actcgggccg ttcggtcatc gtggtcggcc cgacgctgcg cctgcaccag tgcggcctgc
     3001 cgaagaagat ggcgctggag ctgttcaagc ccttcatctt ctcgaagctg atccgccgcg
     3061 gcgtcgcgac gaccatcaag gccgcgaaga agatggtcga gcgcgagcag ccggaagtct
     3121 gggacgtgct cgaagaggtc atccgcgagc atccggtgat gctgaaccgc gccccgacgc
     3181 tgcaccgcct cggcatccag gccttcgagc cggtgctgat cgaaggcaag gcgatccagc
     3241 tgcatccgct ggtgtgcacg gcgttcaacg ccgacttcga cggcgaccag atggccgtgc
     3301 acgtgccgct gtcgatcgag gcgcagctcg aagcgcgcgc gctgatgatg tcgaccaaca
     3361 acatcctgag cccggccaac ggtgatccga tcatcgtgcc gtcgcaggac gtggtgctcg
     3421 gcctctatta catgacccgc gagcgcatca acgcccgcgg cgaaggcatg cgcttcaccg
     3481 acgtgaagga agtgcaccgc gcctacgaga cgcgccaggc cgaactgcag gcgcgcatcg
     3541 aggtgcgcat cgtcgagacg ctgatccacg ccgacggcag ccgcgagcgc gtgcgccggc
     3601 gcgtgagcac gaccgtcggc cgcgcgctgc tgttcgagat cttcccggaa ggcctgccgt
     3661 tcgagctggc caaccaggtg ctgaagaaga agacgatctc gggcctgatc aaccagtgct
     3721 accggcgcct cggcctgaag gacaccgtca tcttcgccga ccagctgatg tacaccggct
     3781 tccactacgc gatgcgttcg ggctcgtcgg tcggcttcac cgacatgcgc atccccgacg
     3841 agaaggccgg catcctggcg cgcgccgaat ccgaggtgaa gcagatcgag gagcagtacg
     3901 cctcgggtct ggtcaccaac ggcgagcgct acaacaaggt ggtcgacatc tggtcgcgca
     3961 ccaacgacga agtcaccaag gcgatgatgc gcggcctcgg caaggagacc gtcgtcaacc
     4021 gcgaaggtca gaccgtcgag caggactcct ttaatagtgt gttcatgatg gccgactccg
     4081 gcgcccgcgg ttcgcaggcg cagatgcgtc agctcgccgg catgcgcggc ctgatggcca
     4141 agcccgacgg ctcgatcatc gagacgccga tcaccgcgaa cttccgcgaa ggcctggacg
     4201 tgctgcagta cttcatctcc acgcacggcg cccgcaaggg tctggccgac acggcgctga
     4261 agaccgcgaa ctccggctac ctgacccgcc gtctggtcga cgtggcccag gatctggtgg
     4321 tcaccgagga agactgcggc acgcgcgaag gcctgctgat gacgccgatc gtcgaaggcg
     4381 gcgatgtcgt cgagccgctg cgcgagcggg tgctcgggcg cgtggtcgcc gaggatgtct
     4441 gccgcccggg cagcggcgag gtcgtggctc cggccggcac gctgctcgac gagcactggg
     4501 tcgacgtgct cgaaagcgcc ggcatcgacc aggtgctggt gcgcagcccc atcacctgcg
     4561 agacccgcta cggcgtctgc tcgtcgtgct acggccgtga cctggcgcgt ggccacatcg
     4621 tcaacatcgg cgaggcggtc ggcgtcatcg ccgcgcagtc gatcggcgag cccggcacgc
     4681 agctgacgat gcgcaccttc cacatcggcg gtgcggcctc gcgtgcggcg gcggtcagca
     4741 gcgtgcaggt caaggcccgc ggccgcatcc gcctgcacaa cctgaagacc gtcaccaaca
     4801 aggacggcaa cctggtcgcg gtgtcgcgtt cgggcgaagt cacggtcatg gacgacaacg
     4861 gccgtgagcg cgagcgctac aagattccgt acggtgccgt gctgacggtc aaggaaggcg
     4921 acagcgtcga ggccggccag atcgccgcga actgggatcc gcacacgcac ccggtggtca
     4981 ccgaggtcga cggtcgcgtg cgcttcctgg acttcgagga gggttcgacc gtcacgcgca
     5041 agaccgacga gatcaccggt ctgtccagcc tcgaggtcct ggacccgaag gcgcgcggct
     5101 cgctcggcaa ggacaagcgt ccgaccgtgc gtctggtcga tgccgccgag cagccgctga
     5161 agctcgccgg caccgacctg ccggcgcagt acccgctgcc gccgggggcg atcgtcaacc
     5221 tcaccgacgg cgccgaggtg cgcgtcggcg acatcgtcgc gcgcattccg caggagtcct
     5281 cgaagacccg tgacatcacc ggcggtctgc cgcgcgtcgc ggatctcttc gaagcgcgca
     5341 agcccaagga gccggcgatc ctcgccgaaa tctcgggcac ggtcagcttc ggcaaggaca
     5401 ccaagggcaa gcagcgcctg gtgctgaccg acaaggatgg cggcacccac gaggaactga
     5461 ttccgaagtg gcgccacgtc aacgtcttcg agggcgagca ggtcgagcgc ggcgaggtca
     5521 tcgccgatgg cgagccgaat ccgatggaca tcctgcgcct gcgcggcgtg cgggatctcg
     5581 ccgcctatct ggtcaaggag atccaggacg tctaccgcct gcagggcgtg aagatcaacg
     5641 acaagcacat cgaggtgatc attcgccaga tgatgcgcaa ggtcgagatc agcgagccga
     5701 acaacgcccg cttcatcaag ggcgagcagg tcgagcgcac gcgcgtgctc gaagagaacg
     5761 atcgcgtgcg cggcgacaac ggcgtgccgg cgcactggca cccgctgctg ctcggtatca
     5821 ccaaggcctc gctggccacc gagtcgttca tctcggcggc ctcgttccag gagaccacgc
     5881 gcgtgctgac cgaggcggcc gtgcgcggct cgcgtgacga cctgcgcggc ctcaaggaga
     5941 acgtcatcgt cggtcgcctg atcccggccg gtaccggact cgcctaccat gccgaccgtc
     6001 gcggcgggcg caccacgccg cgcccgggtt cgtccttcgg caccgagttc agcaccgccg
     6061 aattcggcag cgtcgattac agcgccggcg gcgaaagcca cgccgaggag taagccacac
     6121 gcgccgcccc ggaacggccc cgtcgccgct ccggggcggt gtcatttcgg ggctccggga
     6181 acgcattgac ttttccgggg ttttccgcag ctcagcttga cagggtacag acccgcccct
     6241 aatatcccgc gcctttggcg gacagcgggc ggtcttgcgg cctgtcgtct tgtgttcgag
     6301 gttcaggagt agcaatggca acgatcaacc agctggttcg caagccgcgc agcagcaagc
     6361 ccgaaaagag cgccgtgcca gccctcaacg gctgcccgca gaagcgcggc gtctgcacgc
     6421 gcgtatacac caccaccccg aagaagccga actcggcgct gcgcaaggtt gcccgcgtgc
     6481 ggctgaccaa cggcatggaa gtgaccagct acatcggcgg tgaaggccac aacctgcagg
     6541 agcactccgt ggtcctgatc cgcggcggtc gcgtcaagga cctcccgggt gtgcgctatc
     6601 acacgatccg tggctgcctc gacacctcgg gcgtgcagaa gcgccgtcag ggtcgttcga
     6661 agtacggcgc caagcgtccg aagtcctgac cggcagccgc cgttcctccg cagagtttcc
     6721 agatttccag gtaagcccga gccatgccca gaagaagaga agttccgaag cgcaccatcc
     6781 tcccggatcc gaagttcggg aacgagatgc tggccaagtt catgaacatg gtcatggagc
     6841 gcggcaagaa gtcggtcgcc gagaagatca tctacggcgc gctcgacgcg atcaccgaga
     6901 agggcaagga cgatgcgatg tccgtcgtcg agcaggcgct cgacaacgtg cgtccggccg
     6961 tcgaggtcaa gtcccgccgc gtcggcggtg ccacctatca ggtgccggtc gaggtgcgtc
     7021 aggtgcgtcg caacacgctg gcgatgcgct ggatcatcga tgccgcccgc aagcgtggcg
     7081 agaagtcgat ggcccgtcgt ctggccggcg agctgctcga cgcttccgag agccgcggtg
     7141 cggctgtcaa gaagcgtgag gacacgcatc gcatggcgga tgccaacaag gcgtttgccc
     7201 actaccgctg gtaagcccga ggattccgac cgtggcgcgt aaaaccccca tcgagcgtta
     7261 ccgtaacatc ggtatcagcg cccacatcga cgccggcaag accacgacga ccgagcgcat
     7321 cctgttctac accggtgtga accacaagat cggtgaagtg catgatggcg ccgcgacgat
     7381 ggactggatg gagcaggagc aggagcgtgg catcacgatc acctccgccg cgaccacctg
     7441 cttctggaag ggcatggcgc agcagttccc cgagcaccgc atcaacatca tcgacacgcc
     7501 cggacacgtc gacttcacga tcgaagtcga gcgttcgatg cgcgtgctcg acggtgcgtg
     7561 catggtgtac tgcgccgtcg gtggtgtgca gccgcagtcg gaaaccgtct ggcgtcaggc
     7621 caacaagtac ggtgtgccgc gcatcgcgtt cgtcaacaag atggaccgcg ccggtgccaa
     7681 cttcctgcgc gtcgtgcagc agatgaagga tcgcctgaag gcgaatccgg tgccgctgca
     7741 gatcgcgatc ggtgccgagg atgccttcga gggcatcgtc gacctgatca cgatgaaggc
     7801 catctactgg aacgacgaca cccagggcac gagcttcgag ctgcgtgacg tgccggccga
     7861 tctggcggat ctctgtgccg aatggcgcga gaagctgatc gaggcggcgg ccgaggcgaa
     7921 cgaggagctg atggacaagt acctggaggg cgaggagctc tccgaggacg agatccatgc
     7981 ggccatccgt tcgcgcacga tcgccggcga gatcgtgccg gcgctgtgcg gttcggcgtt
     8041 caagaacaag ggcgtgcagg cgatgctcga cgccgtcgtg cgctacctgc cggcgccggt
     8101 cgatatcccg gccgtgaccg gcacgctcga tgacggcagc gaagccagtc gcgaacccgc
     8161 cgatgccgcg ccgttctcgg cgctggcgtt caagatcgcg accgatccgt tcgtcggcac
     8221 gctgaccttc ttccgcgtct attccggcgt gctcagctcc ggtgactcgg tgctgaactc
     8281 ggtcaagggc aagaaggagc gcatcggccg catcgtgcag atgcacgcga actcccgcga
     8341 ggagatcaag gaagtgctcg ccggcgacat cgctgcggcg gtcggcctga aggacgtgac
     8401 caccggtgac acgctctgcg atcccgagaa gccgatcgtg ctggagcgca tggagttccc
     8461 ggagccggtg atctcggttg cggtcgagcc gaagaccaag agcgaccagg aaaagatggg
     8521 tctggcgctg aaccgtctgg cgcaggagga cccgtccttc cgcgtgcaca ccgacgagga
     8581 atccggtcag acgatcattt cgggcatggg cgagctgcac ctcgaaatca tcgtcgatcg
     8641 catgaagcgc gagttcaagg tcgaggccaa cgtcggtgcg ccgcaggtcg cctaccgcga
     8701 gacgatccgc gcctcggtcg agcaggaagg caagttcgtg cgccagtccg gcggtcgcgg
     8761 ccagtacggc cacgtctggc tgaagatcga gccgcaggaa gccggcaagg gctacgagtt
     8821 cgtcaacggc atcgtcggcg gcgtcgtgcc gcgcgaatac gtgccggcgg tcgacaaggg
     8881 catccaggaa gcgatggccg acggcgtgct ggccggcttc ccggtcgtcg acgtcaaggt
     8941 cacgctgttc gacggctcgt atcacgatgt cgactccaac gaaatggcgt tcaagatcgc
     9001 cggctccatg ggcttcaagg aaggtttccg caaggcgagg ccggccctgc tggagccgat
     9061 catgcaggtc gaggtcgaga cgcccgagga ctacatgggc gacgtgatcg gcgacctgaa
     9121 ccgccgccgt ggcctcgtgc agggcatgga cgactcgccg tcgggcaaga tcgtcaaggc
     9181 cgaggtgccg ctggccgaga tgttcggcta cgcgacctcg ctgcgttcgg cgacgcaggg
     9241 tcgggcgacc tactcgatgg agttcgcgaa gtacaacgag gccccggcga acatcgccga
     9301 ggcggtgatc aagaaggcgt cctgaacgcc cttccggaat tcatgaggta agcagcagtg
     9361 gctaaggaaa aattcgagcg caagaagccg cacgtgaacg tgggcacgat cggtcacgtc
     9421 gacc
//
LOCUS       DAOQIE010000139         9362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3723_length_9362_cov_8.373160, whole genome shotgun sequence.
ACCESSION   DAOQIE010000139 DAOQIE010000000
VERSION     DAOQIE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3723_length_9362_cov_8.373160"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PK144_09935"
     CDS             <1..165
                     /locus_tag="PK144_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF66088.1"
                     /translation="NLKNGQSVVVRINDRGGFQRHGRILDLSPAAARALSMTGAGVAR
                     VGLKRIGGDS"
     gene            complement(182..1114)
                     /locus_tag="PK144_09940"
     CDS             complement(182..1114)
                     /locus_tag="PK144_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF66089.1"
                     /translation="MSPKPTAPDAGPCRLDWDDLRHFLAIARHGTLSGAARALGVRQS
                     TMSRRLAALEARAGAQLLQKTPDGYRPSAAGEAILGPVERIEAEADTVARTITGTDLR
                     LAGSVRITSVETLATLVLMPVLAGFARRYPDIELELLADSRDLDLSRREADVALRLAP
                     YTQPGVVARRLARVGFGVYASADYLDRHGRPDFAAGAPGQYTVLPSDELRQAPETQWF
                     TARTHAARPGLRCNRRQAALAAIEAGMGIGCLARYLGDGRGLVRLDAGPPPPARALWY
                     GVHEDIRHTARIRALAAALAEGFAAAAARLDPPD"
     gene            1264..2103
                     /locus_tag="PK144_09945"
     CDS             1264..2103
                     /locus_tag="PK144_09945"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNF66090.1"
                     /translation="MSAETIPAPGSTPGTAPDAAAALPTLFLSHGSPMLSLLEAPARD
                     FLEALGPELPRPRAILVASAHWETAQPSVNAVERNETIHDFHGFPQALFELRYPAPGS
                     PALAARVADLLHEAGLPCRSDARRGLDHGAWSVLRLMYPDADIPVVQVSLQRGPGPAH
                     HLELGRALAPLRHEGVLVIGSGSFTHDLRRFRGQPIDAPDAPDIAPFCEWMDTALREG
                     RTADLLGYRQRAPYALAQHPSEEHLLPLFVALGAAGPAARARRLHASNTYGMLRMDAY
                     AFA"
     gene            2456..3979
                     /locus_tag="PK144_09950"
     CDS             2456..3979
                     /locus_tag="PK144_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF66091.1"
                     /translation="MPPFRPTAGLTTGVLALALAACTVTPTPVTDGERQARVSADLAQ
                     LTRPVEPVTAPITLDEALRRAVLHNLDQRVKLMETAVAQGQLDVVRWDMLPQLSAQAG
                     YRSRSNDAGSFSKNLSTGVENPDVITSAEDRTRRTAGLVLSWNVLDFGLSYARARQAA
                     DQTLIAAERQRKVLHNIRQDVRQAYWRAWTAQRLLPEVTRLETRTGRALGESREIERQ
                     KLAEPLVALGYQKSLLDTLRELATRRQELELARTELAALMGLPPGTAFRLAPPPANDP
                     VLLALVKTPVEQLIERAFAARPELRQEDYQKRITAMEANKALLGLFPNLNIVGGRSYD
                     SNHYLLNNTWNEVGAGLSLGLLKLFSLPDTLELVERQQQLDELRRLQLGMAIMVQVHL
                     AVQRHALASDDLKLAATAAGVEQRIVGQTRSAVGAQTAHPLDLARAEADGLLAEVRRA
                     LAEAEVQASLARLAASIGEDPAVPGAGTTTAGTPPGPATTPARAADARPRTDAHHAT"
     gene            3969..4700
                     /locus_tag="PK144_09955"
     CDS             3969..4700
                     /locus_tag="PK144_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF66092.1"
                     /translation="MPPERSTLACLLLLAGMSAAADEAVQARALLVPEHEAALAAPMA
                     GRVLEITRREGEAFAKGELLVRFDCAVLEATQRRHRAELGAARRVLDARRRLYRLSSS
                     SELELALAESEVEKAAAQLELVQAQLADCRIEAPYAGRVERREARAYEAVAAGQPLLR
                     VLDDRAIEIRINAPSAWLRWLQPGAAFTVTIDETGATHAARLERLNARVDAASQTIEL
                     IARFTEARPAGLLAGMSGVARFTPP"
     gene            4697..6031
                     /locus_tag="PK144_09960"
     CDS             4697..6031
                     /locus_tag="PK144_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF66093.1"
                     /translation="MTLPPPVTDPALGALVERLATLRELELAARRAPDEAALAFTAVN
                     DSHRLLPYHWAAWCRPGRDGRLQVRALSGLSAVEREAPLVQALEAALADRDLAAADAP
                     RLLPAGTLASDPQLQALWLPLRAPNGGHRLGGLWLARPEAWPPAAQTLLAELAECHAH
                     ALQALSARPPRRSLRTRSLVTLTALALLALGFVPVRLSVLAPAEVVARRPLPVAAPID
                     GVIERFLVQPNEAVEAGAPLFEFDTTTLRARLEVAERAVAVAAAEHQRAVQKAFADAA
                     SRAEVALLAAKLDESRASRDAVAAQLERSRVSAAQAGVAVYTDPDDWLGRPVATGEKI
                     LTLADAADTELAVQVPVDDALILGPGAEVQLFLNTDPLAPLGARLTRSAYEPQPTPEG
                     TLAYPSRAAFDAGTPLPRLGLKGTARVYGEPVTLGYYLMRRPLAALRRWIGL"
     gene            6028..8157
                     /locus_tag="PK144_09965"
     CDS             6028..8157
                     /locus_tag="PK144_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF66094.1"
                     /translation="MNAGAALPPLREELRLEAGPRLPDGAPSWTLHDPLRQRFFRLGW
                     LEVECLRHWGLGSPAAIAGAVAAHTPLAPSPEQVGALADFLAAQQLVRLTPGALHAQR
                     LAQARAARPGLLGWLLHHYLFFRVPLLRPERWLAPLAARLDALYTRTMLVTALVALLL
                     GLYLVGRQWESFTGTFAYFFSAGGLLAWGCALAGSKVLHELGHAVTARRLGVPVPTMG
                     VAFLVLWPVLYTDTTAVWRHPGRRARLAVGAAGMAAELLLAIIATLAWSFLPDGPARS
                     AAFVLASSGWIATLAINASPFMRFDGYFLLSDALDLPNLHERSFALARWQLRRWLFGL
                     DVPAPEPFAPLRHAGLVAFAFATWAYRLVLFLGIALLVYHFAFKPLGIALMAVEIGWF
                     IVRPLAMELRVWWRLRATLMASRRSLLSASVLGAAVLLAILPWHTSLELPATLDAVHV
                     QSLHTPEPARLRRLAVEPGASVAAGDVIAELEQPDLAAELDATGHRVRALREWLRSET
                     SRLAAPERQQRVAEELAGAERAARGLQERQARLTLRAPYAGRVAALADGLLPGVWLSP
                     RQTLAQIIDPAAARIEALVPEDRLTTVARAVADGAAARFHPDTPERPALGALVLALAP
                     AASRTLDAQPLLASVYGGPLAATRDAHGRLLPQTPVYRLDLQPAAGAAAPERRLRGTL
                     VLEVEAEPLLTQVWRRIAAVLIRESGF"
     gene            8278..>9362
                     /locus_tag="PK144_09970"
     CDS             8278..>9362
                     /locus_tag="PK144_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNF66095.1"
                     /translation="MTRNPRRPAGSRSPASLMLALEPRLMFDAAALSSAADTLSHADP
                     AGEAAVAAVEAARASDSPAAAGTASDSAAAAGTATVATAAPAPTADTGTTAPALLAAI
                     ASDSPQTTAADATVASAPAGQRHELAIIDTSLTGWQTLAAGLRDGIEVLTIDGSRDGF
                     AQLADALAGRERGGLDAIHVFSHGSEGQWRLGSSALDVNTEAAHASELQAIAAALAAD
                     GDLLLYGCDTGAGSRGTAFLDRLASATGADVAASTDRSGSATAGGNWTLEAQRGAIET
                     ATAADAAKLADYNGTLATYTVTSTADSGAGSFRQAVLDANASGGTDTIVFDNSLDNQT
                     ITLTGGVISVTDSLIIDGDLDGDDTPDV"
BASE COUNT         1201 a         3617 c         3314 g         1230 t
ORIGIN      
        1 aacctgaaga acggccagag cgtcgtcgtg cgcatcaatg accgcggcgg cttccagcgc
       61 cacggccgca tcctcgacct gtcgccggcg gccgcgcgcg cgttgtcgat gaccggcgcc
      121 ggcgtcgccc gtgtcggcct gaagcggatc ggcggcgaca gctgaagcga ccgctgccgg
      181 ttcagtccgg cggatccagc cgcgccgcgg cggctgcgaa gccctcggcg agggctgcag
      241 ccagcgcgcg gatgcgcgcg gtgtggcgga tgtcctcatg cacgccgtac cacagcgcgc
      301 gcgccggcgg cggcgggccg gcgtcgaggc gcacgagtcc gcggccgtcg ccgagatagc
      361 gcgccagaca gccgatgccc atgccggcct cgatcgccgc cagcgccgcc tgccggcggt
      421 tgcagcgcag gcccgggcgt gccgcgtgcg tgcgcgccgt gaaccactgc gtttccggcg
      481 cctggcgcaa ctcgtccgag ggcagcaccg tgtactggcc cggcgccccg gcggcgaagt
      541 ccggccggcc gtggcgatcg aggtagtccg cactcgcgta cacgccgaag ccgacgcgcg
      601 ccagccggcg cgcgaccacg cccggctgcg tgtacggcgc cagacgcagc gccacgtcgg
      661 cctcgcgccg gctcaggtcg agatcgcggc tgtcggcgag cagttcgagc tcgatgtccg
      721 gatagcgccg tgcgaaaccg gcgagcaccg gcatcagcac cagcgttgcc agcgtctcga
      781 cgctggtgat gcgcacgctg ccggccaggc gcaggtcggt gccggtgatc gtgcgcgcga
      841 cggtgtcggc ctcggcctcg atgcgctcga ccggcccgag gatggcctcg ccggcggcgc
      901 tcggccggta gccgtccggc gtcttctgca gcagttgtgc gccggcgcgg gcttcgaggg
      961 ccgccagacg ccgggacatc gtcgactggc gcacgccgag cgcgcgcgcc gcccccgaca
     1021 gcgtgccgtg ccgggcaatc gcgaggaaat ggcgcaggtc gtcccagtcg agccggcacg
     1081 ggccggcatc cggagcagtc ggcttcggtg acatcgcgcg ctcccgcagc ggtgcatgcg
     1141 ttttcgcaat atgcgttttc gcaaacatag tgcgcggttt tcggcaatgg tgatgcgtcg
     1201 atgcagacca cacgatgcag gtatcgccgc ctgcgtgcgg cccgatgcca tccggagtcc
     1261 gccatgtccg ccgaaaccat ccctgccccg ggcagcactc ctggcacggc ccccgatgcc
     1321 gctgccgccc tgccgacgct gttcctcagc cacggctcgc cgatgctgtc gctgctcgaa
     1381 gccccggcgc gcgatttcct cgaagcgctc ggcccggagc tgccgcgccc gcgcgcgatc
     1441 ctggtcgcct ccgcgcactg ggagacggcg cagccgagcg tcaacgccgt cgagcgcaac
     1501 gagacgatcc acgacttcca cggcttcccg caggcgctgt tcgagctgcg ctatccggcg
     1561 cccggctcgc cggcgctcgc cgcgcgcgtc gccgatctgc tgcacgaagc cggcctgccg
     1621 tgtcgcagcg atgcccggcg cgggctcgat cacggtgcct ggtcggtgct gcggctgatg
     1681 tatccggacg ccgacatccc ggtcgtgcag gtctcgctgc agcgcggccc cgggccggcg
     1741 catcacctcg aactcggccg cgcgctggcg ccgctgcgtc acgaaggcgt gctggtgatc
     1801 ggctcgggca gcttcacgca cgacctgcgc cgcttccgcg gccagccgat cgatgcgccg
     1861 gatgcgcccg acatcgcgcc gttctgcgag tggatggaca cggcgctgcg cgaggggcgc
     1921 acggccgatc tgctcggcta ccgccagcgc gcgccgtacg cgctcgccca gcacccgagc
     1981 gaggaacacc tgctgccgct gttcgtcgcc ctcggcgccg ccggccctgc tgcgcgcgcc
     2041 aggcgcctgc acgccagcaa cacctacggg atgctgcgca tggatgctta cgcattcgcg
     2101 tgaaacacgg cagcggtggt ttgttgcggg tgcacaaccg ccgccgtcgc cttgaagcaa
     2161 tctctgcaag cccgggccgg cgccggtgcc gcatgcccgg gctttgtctt cggccggcct
     2221 ccggcgccgg aaaagcgcga agatgaacgg aaattcaggt ttttccgttt cctgccgaca
     2281 tcccggcaca cgccatccct gccggatccg gtgaggctgg cgccggcgaa gctgtcagga
     2341 atatttacag ttgccgcagc cggcttctgg caaatctctg cgccttcgca accccttgcc
     2401 cggacgcgtc cgccgtgacg cgacccgact caccctgcgt aaggaaacca tgccgatgcc
     2461 tccgtttcga ccgacagccg gcctgaccac cggcgtgctg gcactcgcgc tcgctgcctg
     2521 caccgtcacg ccgacccccg tgaccgatgg cgagcgacag gcgcgcgtct cggccgatct
     2581 ggcgcagctg acccggccgg tcgagccggt caccgcgccg atcacgctcg acgaggcgct
     2641 gcgccgggcg gtcctgcaca acctcgacca gcgcgtgaag ctgatggaaa ccgcggtcgc
     2701 gcagggtcag ctcgacgtgg tgcgctggga catgctgccg cagctgagcg cgcaggccgg
     2761 ctaccgctcg cgcagcaacg acgccggctc gttcagcaag aacctctcga ccggcgtcga
     2821 gaaccccgac gtgatcacct cggccgagga ccgcacgcgg cgcacggccg gactggtgct
     2881 gagctggaac gtgctcgatt tcgggctttc gtacgcgcgc gcccgccagg ccgccgacca
     2941 gacgctgatc gctgccgaac gccagcgcaa ggtgctgcac aacatccgtc aggacgtgcg
     3001 ccaggcctac tggcgggcct ggaccgccca gcgcctgctg cccgaagtca cgcgcctcga
     3061 aacgcgcacc ggccgcgcgc tcggcgaatc gcgcgagatc gagcgccaga agctcgccga
     3121 accgctggtc gcgctcggct accagaaatc cctgctcgac acgctgcgcg aactcgcgac
     3181 ccgccgtcag gaactcgaac tggcgcgcac cgaactcgcg gcgctgatgg gcctgccgcc
     3241 cggcaccgcc ttccggctcg cgccgccgcc ggcgaacgat ccggtgctgc tcgcgctggt
     3301 gaagacgccg gtcgagcagc tgatcgagcg cgccttcgcc gcgcgcccgg agctgcgcca
     3361 ggaggactac cagaagcgca tcacggcgat ggaggcgaac aaggccctgc tcgggctgtt
     3421 cccgaacctc aacatcgtcg gcgggcgcag ctacgacagc aaccactacc tgctcaacaa
     3481 cacctggaac gaggtcggtg ccggcctgtc gctcgggctg ctgaagctgt tctcgctgcc
     3541 cgacacgctg gagctggtcg agcgccagca gcagctcgac gagctgcgcc ggctgcagct
     3601 cggcatggcg atcatggtgc aggtgcatct ggccgtgcag cgccacgcgc tcgccagcga
     3661 cgacctgaag ctcgccgcca ccgccgccgg cgtcgagcag cgcatcgtcg gccagacgcg
     3721 cagcgccgtc ggggcgcaga ccgcgcaccc gctcgacctg gcgcgcgccg aggccgacgg
     3781 cctgctcgcc gaggtgcgcc gcgcgctcgc cgaggccgag gtgcaggcga gcctcgcgcg
     3841 gctcgccgcc agcatcggtg aggacccggc cgtgccgggc gccggcacca cgacggcggg
     3901 cacgccgccc ggccccgcca cgacgccggc ccgtgctgcc gatgccaggc cgcgcacgga
     3961 tgcgcaccat gccacctgaa cgaagcacgc tcgcctgcct gctgctgctc gcgggcatga
     4021 gcgccgcggc cgacgaggcc gtgcaggcgc gggcgctgct ggtgcccgag cacgaggcgg
     4081 cgctggctgc gccgatggcc gggcgcgtgc tggagatcac gcggcgcgag ggcgaggcct
     4141 tcgccaaggg cgagctgctg gtgcgcttcg actgcgccgt gctcgaagcc acgcagcggc
     4201 gccaccgcgc cgagctcggc gccgcgcgcc gcgtgctcga tgcgcgccgg cgcctgtacc
     4261 ggctcagctc cagcagcgag ctcgaactgg cgctcgccga gagcgaggtc gagaaggccg
     4321 ctgcccagct cgaactggtg caggcgcagc tcgccgactg ccgcatcgag gcgccgtacg
     4381 ccgggcgcgt cgaacgccgc gaggcacgcg catacgaggc ggtcgccgcc ggccagccgc
     4441 tgctgcgcgt gctcgatgac cgcgcgatcg agatccgcat caacgccccc tcggcctggc
     4501 tgcgctggct gcagccgggg gcggcgttca cggtcacgat cgacgagacc ggcgccacgc
     4561 acgcggcgcg cctcgaacgc ctgaatgccc gcgtcgatgc cgcgagccag accatcgaac
     4621 tgatcgcccg cttcaccgaa gcccggccgg ccggactgct ggccggcatg agcggcgtgg
     4681 cccggttcac gccgccatga cgctgccgcc gcccgtgacc gatccggccc tcggcgcgct
     4741 ggtcgagcgg ctggcgacgc tgcgcgaact cgaactcgcg gcgcggcgcg cgcccgacga
     4801 ggcggcgctg gcgttcacgg ccgtcaacga cagccaccgg ctgctgcctt atcactgggc
     4861 ggcgtggtgc cggcccgggc gcgacggccg cctgcaggtg cgcgcgctgt cggggctttc
     4921 ggccgtcgag cgcgaggcgc cgctggtgca ggcgctcgaa gccgcgctcg ccgaccggga
     4981 tctggccgcg gccgatgctc cgcgcctgct gccggccggc acgctggcga gcgatccgca
     5041 gctgcaggcg ctgtggctgc cgctgcgcgc gccgaatggc gggcaccggc tcggcggcct
     5101 ctggctcgcg cgtcccgagg cctggccgcc ggcagcacag acgctgctcg ccgaactcgc
     5161 cgagtgccac gcccacgccc tgcaggcgct gtcggcccgg ccgccgcgcc ggtcgctgcg
     5221 cacgcgcagc ctcgtcacgc tgacggcgct ggcgctgctc gcgctcggct tcgtgccggt
     5281 gcgcctgtcg gtgctggcgc cggccgaggt ggtcgcccgc cggccgctgc cggtcgccgc
     5341 cccgatcgac ggcgtcatcg agcgcttcct cgtgcagccg aacgaggcgg tcgaggccgg
     5401 ggcgccgctg ttcgagttcg acacgaccac gctgcgcgca cgcctcgaag tcgccgagcg
     5461 cgcggtcgcg gtcgcggccg ccgagcatca gcgtgcggtg cagaaggcgt tcgccgatgc
     5521 cgcgagccgt gccgaggtgg cgctgctcgc cgcgaagctc gacgagagcc gcgcctcgcg
     5581 cgatgccgtc gccgcgcagc tcgaacgcag ccgcgtcagc gccgcgcagg ccggcgtcgc
     5641 cgtctacacc gatcccgatg actggctcgg gcgaccggtc gccaccggcg agaagatcct
     5701 gacgctcgcc gatgcggccg acaccgagct ggccgtgcag gtgccggtcg acgatgcgct
     5761 gatcctcggc cccggcgccg aggtgcagct gttcctgaac accgacccgc tggccccgct
     5821 tggcgcccgg ctgacgcgca gcgcctacga gccgcagccg acgcccgagg gcacgctcgc
     5881 ttacccgtcg cgcgccgcct tcgacgccgg cacgccgctg ccgcgcctcg gcctcaaggg
     5941 cacggcgcgg gtctacggcg agccggtgac gctcggttac tacctgatgc gccggccgct
     6001 ggcggcgctg cgccgctgga tcgggctgtg aacgccggcg ccgcactgcc gccgctgcgc
     6061 gaggagctgc gcctcgaagc cggcccgcgc ctgcccgatg gcgcgccgag ctggacgctg
     6121 cacgacccgc tgcgccagcg cttcttccgc ctcggctggc tggaggtcga gtgcctgcgc
     6181 cactgggggc tcggctcgcc ggcggcgatc gccggggcgg tcgccgcgca cacgccgctc
     6241 gcaccctcgc cggagcaggt cggcgcgctc gccgacttcc tcgccgcgca gcagctcgtg
     6301 cgcctgaccc ccggcgcgct gcacgcgcag cgcctcgcgc aggcgcgcgc cgcgcgcccc
     6361 ggcctgctgg gctggctgct gcaccactac ctgttcttcc gcgtgccgct gctgcgcccc
     6421 gagcgctggc tggcgccgct cgcggcccgg ctcgatgcgc tgtacacgcg cacgatgctg
     6481 gtcacggcgc tcgtggcgct gctgctcggc ctgtatctgg tcggccggca gtgggagagc
     6541 ttcaccggca ccttcgcgta cttcttcagt gccggcggcc tgctcgcctg gggctgcgcg
     6601 ctcgccggca gcaaggtcct gcacgaactc ggtcacgccg tcaccgcacg gcgcctcggc
     6661 gtgccggtgc cgacgatggg cgtggccttc ctggtgctgt ggccggtgct gtacaccgac
     6721 acgaccgcgg tctggcgcca tcccgggcgc cgcgcccggc tcgcggtcgg tgctgccggc
     6781 atggccgcgg agctgctgct cgcgatcatc gcgacgctgg cgtggagctt cctgcccgac
     6841 ggcccggcgc gctcggcggc cttcgtgctc gcgagcagcg gctggatcgc gacgctcgcg
     6901 atcaacgcca gcccgttcat gcgcttcgac ggctacttcc tgctcagcga tgcgctcgac
     6961 ctgccgaacc tgcacgagcg cagcttcgcg ctggcgcgct ggcagctgcg ccgctggctg
     7021 ttcggactgg acgtgccggc gccggagcct tttgcgccgc tgcgtcacgc cggactggtc
     7081 gcgttcgcgt tcgcgacctg ggcttaccgg ctggtgctgt tcctcggcat cgcgctgctc
     7141 gtctatcact tcgcgttcaa gccgctcggc atcgcgctga tggcggtgga gatcggctgg
     7201 ttcatcgtcc ggccgctggc gatggagctg cgcgtgtggt ggcggctgcg cgcgacgctc
     7261 atggccagcc gccgcagcct gctgagcgcg agtgtgctcg gtgccgccgt gctgctggcg
     7321 atcctgccct ggcacacgag cctcgaactg ccggcgacgc tcgatgccgt gcacgtccag
     7381 agcctgcaca cgcccgagcc ggcgcggctg cggcggctgg cggtcgagcc cggtgcgagc
     7441 gtcgcggccg gcgacgtgat cgccgaactc gaacagcccg atctcgccgc cgaactcgac
     7501 gccaccggcc accgcgtgcg ggcgctgcgc gaatggctgc gcagcgagac cagccggctc
     7561 gccgcccccg agcgccagca gcgcgtcgcc gaggaactcg ccggcgccga gcgcgccgcg
     7621 cgcggcctgc aggaacgtca ggcgcggctg acgctgcgcg cgccctacgc cgggcgtgtt
     7681 gccgcgctcg ccgatggcct gctgcccggc gtctggctgt cgccgcgcca gacgctcgcg
     7741 cagatcatcg acccggctgc ggcgcgcatc gaggcactgg tgcccgaaga ccggctcacg
     7801 accgtcgccc gtgccgtcgc cgacggtgcc gcggcacgct ttcatcccga cacccccgag
     7861 cgcccggcgc tcggcgcgct cgtgctcgcc ctcgctccgg ccgccagccg cacgctcgat
     7921 gcgcagccgc tgcttgcctc cgtgtacggc gggccgctcg ccgcgacccg cgatgcccac
     7981 ggccggctgc tgccgcagac gccggtctac cggctcgacc tgcagcccgc cgccggggcc
     8041 gctgcgcccg agcgccggct gcgcggcacg ctggtgctcg aagtcgaggc cgagccgctg
     8101 ctgacgcagg tctggcgccg gatcgccgcg gtgctgatcc gtgagagcgg cttctgaacc
     8161 ggccggcgcc cggcgccggt ggcggatgcg cctgaaccgc accctgaccc gaaccttgcc
     8221 ccgaactccg gcacgcccga tggcgccggc acgaactcgc aggactggag agccgacatg
     8281 acccgcaacc cccgtcgccc tgccggcagc cgcagccccg cgagcctgat gctggcgctg
     8341 gagccgcgcc tgatgttcga cgccgccgcc ctcagcagcg ccgcggacac actcagccac
     8401 gccgacccgg ccggcgaagc ggcggtggcc gcggtcgagg ccgccagagc cagcgacagc
     8461 ccggctgcgg ccggcacggc cagcgacagt gccgcggccg ccggtactgc gaccgtcgcc
     8521 accgccgcgc cggccccgac ggccgacacc ggtacgaccg caccggcact gctggcagcg
     8581 atcgcgagcg attcgccgca gacgacggct gcggacgcca ccgtggccag cgccccggcc
     8641 gggcagcgcc atgagctggc catcatcgac acctcgctca ccggctggca gacactcgcc
     8701 gccggcctgc gtgacggcat cgaggtgctg acgatcgacg gcagccgcga cggcttcgcg
     8761 cagctcgccg acgcgctcgc cgggcgcgag cgtggcgggc tcgatgccat ccacgtcttc
     8821 agccacggca gcgagggcca gtggcggctc ggcagcagcg cgctcgatgt caacaccgag
     8881 gccgcacacg cgagcgaact gcaggccatc gctgccgcgc tcgccgcgga tggcgacctg
     8941 ctgctctacg gctgcgacac cggcgccggc agccgcggca cggccttcct cgatcgcctt
     9001 gccagcgcca ccggcgccga cgtggccgcc tcgaccgatc gcagcggcag cgccacggcc
     9061 ggcggcaact ggacgctgga agcgcagcgt ggcgcgatcg aaaccgcgac ggcggccgat
     9121 gccgcgaagc ttgcggatta caacggcacg ctggcgacct acacggtcac gagcaccgcc
     9181 gacagcggcg ccggcagctt ccgtcaggcc gtgctcgatg ccaacgccag cggcggcacc
     9241 gacaccatcg tgttcgacaa cagcctcgac aaccagacga tcacgctcac gggcggcgtc
     9301 atcagcgtga ccgacagcct gatcatcgat ggcgacctcg acggcgacga cacgccggat
     9361 gt
//
LOCUS       DAOQIE010000140         9359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3725_length_9359_cov_6.978611, whole genome shotgun sequence.
ACCESSION   DAOQIE010000140 DAOQIE010000000
VERSION     DAOQIE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_9359_cov_6.978611"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /locus_tag="PK144_09975"
     CDS             <1..1778
                     /locus_tag="PK144_09975"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNF66096.1"
                     /translation="GGEVLQIQSLEVPVAYYNNKLKLIAGEGGKFTLLDPDKNPVLEG
                     EVGKPAETRIGHETLRIFVSDLQARPGVTFKVYKFDVLTALKNLTDNLKAAETAKSSG
                     IISVSYSDYDPVLAVRIVSEMANLYLRQNVERKSADAQQTLSFLDKHLPLLKQNMDNA
                     IATLNAYRIKAGSVDLQKETLDVLDQSVTLQSDIVKLRQKRDELMQRFTASHPQVIAI
                     DAQIARLSNLLKDIDRKVQVLPDAQKEILQLERDAKVATDLYITLLNNAQQLQVAKAG
                     TVGNVRIIDYPLVPIEQDKPKVPLVIAIAVGLGIFLGVISAFVRHALRGGVEDPDLIE
                     KGLGLPVYATIPHSKLQDKLSRKLHNNSGLNLILFSQDKDDLAIESLRSLRTTLHFVL
                     MEAKNNVIMVTGPSPGLGKSFVSMNLGAVLASAGRRTLIIDADLRRGHLHEYTGLPRG
                     QGVSDLVSGQVDVYGAIRPTLVQNLEIMSTGAVPPNPSELLLHEKFAQMIGEVSKQYD
                     HVLIDSPPVLAVTDAAVVGRLAGASLLVVKAGANPMREIEVSVKRLRQAGINLRGVLF
                     NNVTKIGSRYGAGKYVYQYAYTKSK"
     gene            1928..2944
                     /gene="galE"
                     /locus_tag="PK144_09980"
     CDS             1928..2944
                     /gene="galE"
                     /locus_tag="PK144_09980"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNF66097.1"
                     /translation="MSLILLTGGAGYIGSHTAVELLARGHEVLLVDNFCNSSPRVLER
                     LARITGQSVACERIDVRDANALADVFARHPVDAVIHFAALKAVGESCTQPLRYFDNNI
                     GGTIGLLQSMQAAGVSRLVFSSSATVYGQPEACPITESAPLRVTNPYGRTKLVMEELI
                     GDVCTAHPDFHAAILRYFNPVGAHESGLIGEEPTGIPNNLMPFVCQVAAGRREFLSVF
                     GGDYPTRDGTGVRDFIHVVDLAKAHVAAVDRLLRVPESMTVNLGTGCGYSVLELVQSF
                     ERASNRRIPYRIVGRRAGDVAECYADPRQAHEILAWRAEFGIERMCTDAWRWQSMFPR
                     GYAD"
     gene            3056..4774
                     /locus_tag="PK144_09985"
     CDS             3056..4774
                     /locus_tag="PK144_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNF66098.1"
                     /translation="MLAVSRIGPDMILAAAMTVLLVSGVLKPGEALSGFGNPGVLTVA
                     VLFVTVAGLRETGGIQWIAAFVLGRPGALGRAQFRLIGTSSVLSAFVNNTPIVAMLTP
                     AVQEWSRRAGHAPSKLLLPLDYATILGGMCTLIGTSTNLVVNGLIARQGLAPLGMFDP
                     MWVGLPCVLVGGLYLMTVGRWLLPARRSAVEQVANAREYTLEMIVEEQGPLVGLSVEE
                     AGLRALPQLYLIEIVREGRVVQAIAPQEILRADDRLVFVGIVDSVLDLRRMRGLRPAT
                     DQIFKLAVRHNQRCLIEAVVSPVSPCVGRTIRESRFRNRYNGAVIAVSRRGERVHRKL
                     GDIVLQAGDTLLIEAGAQFARHHRYARDFLLVSAVDESTATDHSRAPIALGILAALVI
                     GTTVFDVDTLYGALLAAAAMIATRCVNQGQARRSIDYQLIVIIACSFALGLAIEKTQV
                     AKLVAQLMMLSVSNDPWGTLAVIYVLTVLATELLTNNAAAVLMFPIGVAAARQLGVSE
                     LPFVMSVMVGASAGFISPIGYQTHLMVMGPGGYRFSDFFRIGTPLSVLVGLTTIVVAP
                     LAWPFR"
     gene            4918..6630
                     /locus_tag="PK144_09990"
     CDS             4918..6630
                     /locus_tag="PK144_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNF66099.1"
                     /translation="MTTLIAPHGGTLKNLYLTPELAAAEKQKAKELLSWDLDHRQLCD
                     IELLLNGAFSPLEGFLGRADYDRVVSEMRLADGTLWPMPIMLDVSEAFAAQVQVGATI
                     ALRDPEGVLIATMEIADLWTPDKALEAQNVFGTTDEKHPGVAYLMRKAGPVYIGGKLK
                     GVEEPMHYDFRSLRNSPAEMRALFNKLGWRKIVAFQTRNPMHRAHQELTFRAAKLAQA
                     NLLIHPVVGMTKPGDIDHYSRVRCYEHLIKQYPEQSTMLSLLHLAMRMGGPREAVWHA
                     IIRKNHGCTHFIVGRDHAGPGKNSKGKDFYGPYDAQEMVAQYAEELGIEMVPFQEMVY
                     VEELAEYVPQDEVKPGQTVLNISGTEFRRRLMEGLEIPAWFSYPEVVAELRRTHPPKH
                     LQGFTVFFTGLSGSGKSTIANALMVKLMELGGRRVTMLDGDLVRKNLSSELGFSKEHR
                     NINILRIGYVASEITKNGGIAICAPIAPYTQTRKDVRDMIAPLGGFIEIHVATTVETC
                     EQRDRKGLYAKARAGIIKEFTGISDPYEAPENPEMKIQTEECTPDEAAQRILLKLESL
                     GYIR"
     gene            6713..7597
                     /gene="rfbA"
                     /locus_tag="PK144_09995"
     CDS             6713..7597
                     /gene="rfbA"
                     /locus_tag="PK144_09995"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNF66100.1"
                     /translation="MDRKGLILAGGSGTRLHPLTLSVSKQLMPVYDKPMIYYPLTTLM
                     LAGIREILIITTPRDQEAFQALLGDGSQWGVRLEYAVQPSPDGLAQAFLIGEEFLAGS
                     PAALVLGDNIYHGHGLPELLRRADAREQGATVFGYYVRDPERYGVVEFDAAGRAVSVE
                     EKPAQPKSNYAITGLYFYDADVVEVAKAVRPSARGELEITDVNRIYLERGQLNVELMG
                     RGYAWLDTGTHDSLLDAANFIQVMQARQGLQIACPEEIAWRLGWIDAEHLERMAKPLA
                     KNGYGRYLLDLLRQGVRP"
     gene            7594..8160
                     /gene="rfbC"
                     /locus_tag="PK144_10000"
     CDS             7594..8160
                     /gene="rfbC"
                     /locus_tag="PK144_10000"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNF66101.1"
                     /translation="MKLIETALPGVLILEPQVHGDARGFFVETYRASWFEAHGLTAPV
                     FVQDNQSRSRRGVLRGLHYQTVNPQGKLVRCARGAVFDVAVDVRRGSPHFGQSVGVVL
                     DDESHRQLWIPPGFAHGFCVLSDVADFAYKCTSYYDPASDAGIRWNDPALGIDWPLED
                     IEPLLSAKDQLLPLLAGQADDKLTVYGG"
     gene            8165..9043
                     /gene="rfbD"
                     /locus_tag="PK144_10005"
     CDS             8165..9043
                     /gene="rfbD"
                     /locus_tag="PK144_10005"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNF66102.1"
                     /translation="MSILVTGANGQVGYELVRRAGPLPVTGLTRSQLDICNAQAVQAA
                     IGQHEARIVINAAAYTAVDKAETEPQAAFAVNRDGPATLAAACAAAGIPLLHLSTDYV
                     FDGSKPAPYTEDDPVAPLGTYGASKLAGEDAIRASGCAHLILRVAWVFGAHGNNFVRT
                     MLRLGREREQLRVVADQLGGPTPAAAIADALLQLAGRHLAGEALPNGTWHFAGVPHTS
                     WHGFANAALEAAAARGLLARVPRIDAITSAEYPTPARRPANSRLDSTRAETGLGLDVP
                     DWRAGLASILDEWKSA"
     gene            9040..>9359
                     /locus_tag="PK144_10010"
     CDS             9040..>9359
                     /locus_tag="PK144_10010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF66103.1"
                     /translation="MSYTPRNVLVTGGAGFIGANYVRWLLGEDAGVHIVNLDALTYAG
                     STGNLKGLPGEERHTFVQGDICDAALVARLLREHAIDTVVHFAAESHVDRSISGPGEF
                     VRTNV"
BASE COUNT         1744 a         2815 c         2943 g         1857 t
ORIGIN      
        1 ggggtggtga agttctgcag atacaaagtc tcgaagtccc ggtcgcgtac tacaacaaca
       61 aactcaagct gattgcaggt gaaggcggca agttcacgct gcttgatccg gacaagaatc
      121 ccgtacttga gggtgaggtc ggtaaaccgg ctgaaacaag gattggtcat gaaaccttgc
      181 ggatatttgt ttccgatttg caggcacgac caggcgtgac tttcaaggtt tacaaattcg
      241 atgtgctgac tgcactgaag aatctcactg acaatctcaa ggcagccgag accgccaagt
      301 catccggtat catctcggta tcttacagtg attatgatcc ggtgctcgca gtccgtattg
      361 tcagtgaaat ggcgaatctg tatctgcgcc agaatgttga gcgcaagtct gccgatgcac
      421 agcaaacact cagttttctg gacaagcacc tgccactgct gaagcagaat atggataatg
      481 cgatcgcgac cttgaatgcg tatcgcatca aggcaggttc cgtcgatctg caaaaggaaa
      541 cactcgacgt tcttgatcag tcggtcactc ttcagtcaga tattgtcaag ttgcgtcaga
      601 aacgcgatga gttgatgcag cggttcacgg catcgcatcc tcaggtgatt gcaatcgatg
      661 cccagattgc gcgattgagc aacctgctga aagatatcga tcgcaaggtc caggtattgc
      721 cggacgcgca gaaagaaatt ctgcaacttg agcgtgacgc caaggtggcg accgatctgt
      781 atatcacgtt gctgaacaac gcccagcagt tgcaggtagc caaggcgggc actgtcggaa
      841 acgtgcgcat catagactat ccactggtgc cgattgaaca ggacaagccg aaggtgcccc
      901 tggtcattgc cattgccgtt gggcttggca tttttttggg cgtcatttcg gctttcgtac
      961 gacatgcgct acgtggtggc gtcgaagatc cggacttgat cgagaaaggc ctgggtttac
     1021 cggtctatgc aacgattccc cacagcaagt tgcaggacaa gctcagtcgt aaactgcata
     1081 ataattccgg tctcaacctg atcctgttca gtcaggacaa ggatgatctg gcgatcgaga
     1141 gtttgcgcag tctgcgcacg acgctgcatt ttgtgctgat ggaagccaag aacaacgtca
     1201 tcatggtgac cggccccagt ccgggactcg gcaaatcttt cgtcagcatg aacctcggtg
     1261 cggtcctggc gagtgctggc cgcagaacat tgatcatcga cgccgatttg cgccgcggcc
     1321 atctgcatga atatacggga ttgccacgcg gtcagggcgt gtccgatctc gtcagtggac
     1381 aagtggatgt ctatggggcg atccggccga cgctggtcca gaatcttgag atcatgagta
     1441 ctggcgcagt cccgccgaat ccctctgaac tgctgctgca cgaaaagttt gcccagatga
     1501 tcggcgaggt ttcaaaacag tacgatcatg tattgatcga ttcaccgccg gtgctggccg
     1561 tgaccgatgc agcagtcgtt ggtcggctgg ccggtgcatc gctgcttgtc gtcaaggccg
     1621 gagcgaatcc gatgcgggaa attgaagtta gcgtcaagcg tctgcgacag gccggcatca
     1681 atcttcgagg ggtattgttc aataatgtga cgaagatcgg cagtcgctac ggggccggca
     1741 agtatgttta tcagtacgca tataccaaga gcaaataagc gccggacggg ccccacaggt
     1801 aaaaatcgat cgtgatgcaa gatgcaggtg accgctgtga tccggtgtct ggcgacaccg
     1861 gtcgtcagtg cattcgagtg accttttgca tgcctgacag gtcgattcac cttggtcgag
     1921 gtggctgatg tcgctgatcc tgctgacggg cggtgccgga tacatcggtt cgcatacggc
     1981 cgtcgagctg ctcgctcgcg ggcatgaagt cctgctcgtt gacaatttct gcaacagttc
     2041 gccgcgggtg ctcgaacggc ttgcacggat cactggccag tccgtggctt gcgaaagaat
     2101 cgatgtgcgt gatgcaaacg cgctggcaga tgtctttgca cgccatcccg ttgatgcagt
     2161 catacatttt gcggctctca aggccgttgg cgaatcgtgc acgcagccgc tgcgttattt
     2221 cgataacaat atcgggggca cgatcggact gctgcagtcg atgcaggctg ccggggtttc
     2281 caggttggtg ttcagttcat cggcgaccgt ctacgggcag cccgaggcct gcccgatcac
     2341 cgaaagtgca ccactgcggg tcacgaatcc ctatggccga accaagctgg tcatggagga
     2401 gctgatcggt gatgtctgta cggctcatcc ggattttcac gccgcaatcc tgcgttactt
     2461 caatccggtc ggggctcacg aaagtggcct gatcggtgag gagccgacgg gtattcccaa
     2521 caacctgatg ccttttgtct gtcaggtggc tgcggggcgg cgcgagtttc tgagtgtgtt
     2581 cggcggtgat tatccgaccc gggacggcac gggcgtacgc gatttcatac acgtcgtcga
     2641 tcttgcgaaa gcccatgttg ccgcggtcga tcgtctgctg cgtgtgcctg aaagcatgac
     2701 ggtcaatctc ggtaccggtt gcggttacag cgtgctggag ctcgtgcagt ccttcgagcg
     2761 ggcaagtaac cggaggattc cttatcgcat cgtcggccgg cgtgccggcg atgttgccga
     2821 atgctatgcg gacccacggc aggcacatga aattctggcc tggcgggcgg agttcggtat
     2881 cgagcgaatg tgtaccgatg catggcgctg gcagtcgatg ttccccaggg gctacgccga
     2941 ctgacgctcc ggctctgttc agacgatcga gggagattcc ggtttggcag tacatgacta
     3001 tgcactggac tggcagggct ggctgacgat cgccgtggtg ctctggacgc tggtcatgct
     3061 ggcggtcagt cgcatcggac cggacatgat cctggcggcg gcgatgacgg tactgctggt
     3121 ttcgggggtg ctgaaaccgg gtgaagcact cagcggcttc ggcaatcccg gcgtgctgac
     3181 ggtggcggtg ctgttcgtga ccgtggccgg attgcgtgag acaggcggca tccagtggat
     3241 tgccgcgttc gtgctgggca ggcccggtgc cctcggtcgc gcgcagtttc ggctgatcgg
     3301 caccagcagc gtgctcagtg ccttcgtcaa caatacgccg atcgtggcga tgctgacgcc
     3361 ggcggtgcag gaatggagtc gccgtgccgg acatgccccg tcgaaactgc tgctgccact
     3421 cgactatgcg acgattcttg gcggcatgtg cacgctgatc ggcacgagta cgaatcttgt
     3481 tgtcaatggt ctgatcgcgc ggcaagggct tgcacctctg ggcatgttcg atccgatgtg
     3541 ggtgggtctg ccgtgtgtgc tggtcggcgg cctttatctg atgacggtcg gccgctggct
     3601 gctgccggcc cgacgttcgg cagtcgagca ggttgccaat gcgcgtgagt acacgctcga
     3661 aatgatcgtc gaggagcagg gaccgctggt cgggctgagc gtcgaagagg ccggcctgcg
     3721 cgcgctgccg cagctctatc tgatcgagat cgtgcgtgag ggcagggtgg tgcaggcgat
     3781 tgccccgcag gaaatcctgc gcgcggatga ccggctggtt ttcgtcggca tcgtggattc
     3841 ggtgcttgat ctgcgccgca tgcgtggctt gcgcccggca accgaccaga tcttcaaact
     3901 ggccgtccgc cataatcagc gttgcctgat cgaagccgtg gtctcgccgg tttcaccctg
     3961 tgtcggacgc acgatccggg aatcccgttt tcgcaatcgc tacaacggcg cggtcatcgc
     4021 ggtttcacgg cgcggcgaac gcgtgcatcg caaactcggc gacatcgtgc tgcaggccgg
     4081 cgatacgctg ttgatcgagg caggtgccca gtttgcgcgg caccatcgct atgcgcgtga
     4141 ctttctgctg gtcagcgctg tcgatgaatc gaccgccacc gatcattcac gggcaccgat
     4201 cgcactcggc attctcgcag ctctggtgat cggcacgacc gtatttgacg tcgataccct
     4261 ctatggtgcc ctgctggcag ccgcagcgat gatcgcgacg cgttgcgtca atcagggcca
     4321 ggcacgtcgt tcgatcgatt accagctcat cgtcatcatt gcctgctcgt tcgcgctcgg
     4381 actggcgatc gagaagaccc aggttgcaaa gctggttgca cagctgatga tgttgtcggt
     4441 cagcaacgat ccctggggca cgctcgcggt gatctacgtg ctgacggtgc tggcgaccga
     4501 gctgctgacc aacaatgccg cagccgtgct gatgtttccg atcggcgtgg ctgcagcacg
     4561 ccagctcggt gtcagcgaac tgccgttcgt catgagcgtg atggtcggcg cctcggctgg
     4621 tttcatctcg ccgatcggct accagacaca tctgatggtc atggggccgg gcggttatcg
     4681 cttctcggat ttcttccgga tcggcacgcc gctcagcgtg ctggttggtc tgaccacaat
     4741 cgtcgtcgcg ccgctcgcat ggccgttccg ctgaacgaca tgcacaacat gtgtggccga
     4801 cccgtgcata ccgctgcccc tgttgttctg tccgccgggg ctcatggggt atgctgcatt
     4861 gcagtagcgc cgctgtgcgg cgctcccgat tcaacgacct gatggaagcc tgatcgcatg
     4921 acgacgctga ttgcccccca tggcggcacc ctcaagaacc tgtatctgac gcccgaactg
     4981 gctgcagccg agaagcagaa ggcgaaggaa ctgctgtcct gggatctgga ccaccgccag
     5041 ctctgtgaca tcgaactgct gcttaacggt gcgttttcgc cgcttgaggg ctttctcggg
     5101 cgtgccgact atgaccgcgt ggtcagcgaa atgcggctgg ccgacggtac gctgtggccg
     5161 atgccgatca tgctcgatgt cagcgaggcg tttgcggccc aggttcaggt cggcgcgacg
     5221 atcgcattgc gcgatcccga gggcgtgctg atcgcgacga tggagattgc cgatctgtgg
     5281 acgccggaca aggcgcttga agcgcagaac gtgttcggca cgaccgacga gaaacacccg
     5341 ggtgtcgcct acctgatgcg caaggccggc ccggtctaca tcggtggcaa gctcaagggc
     5401 gtcgaagagc cgatgcacta cgacttccgc agcctgcgca actcgccggc cgaaatgcgg
     5461 gcgctgttca acaagctcgg ctggcgcaag atcgttgcgt tccagacgcg caatccgatg
     5521 catcgcgcgc atcaggaact gaccttccgc gcggccaagc ttgcccaggc caacctgctg
     5581 atacaccccg tcgtcggcat gaccaagccc ggtgatatcg accattattc gcgcgtgcgc
     5641 tgctacgagc acctgatcaa gcagtatccg gagcagagca cgatgctgtc gctgctgcat
     5701 ctggcgatgc gcatgggcgg accgcgggag gccgtctggc acgcgatcat ccgcaagaat
     5761 cacggctgca cgcatttcat cgtcggtcgt gaccatgccg gtccgggcaa gaattcgaaa
     5821 ggcaaggatt tctacggtcc gtacgatgcg caggaaatgg tggcgcagta cgccgaagaa
     5881 ctcggtatcg agatggtgcc gttccaggag atggtctacg tcgaggaact ggccgagtat
     5941 gttccgcagg acgaggtcaa gccgggtcaa accgtgctga acatttccgg caccgagttc
     6001 cgtcgccgtc tgatggaagg gctggaaatt ccggcctggt tctcgtatcc ggaagtggtc
     6061 gccgaactgc gccgtacgca tccgccgaag cacctgcagg gcttcacggt gttcttcacg
     6121 gggctgtccg gttccggcaa gtcgaccatt gccaacgccc tgatggtcaa gctgatggaa
     6181 ctcggcggcc ggcgggtgac gatgctggac ggcgatctgg tgcgcaagaa cctgtcttcg
     6241 gaactcgggt tcagcaagga gcaccgcaac atcaatatcc tgcgcatcgg ctatgtcgcc
     6301 agcgagatca ccaagaacgg tggcatcgcg atctgtgcgc cgatcgcacc ctatacacag
     6361 acccgcaagg atgtgcgcga catgatcgcg ccgctcggcg gtttcatcga aatccacgtt
     6421 gcgaccacgg tcgaaacctg cgagcagcgt gatcgcaagg gcctgtatgc caaggcccgc
     6481 gccggcatca tcaaggaatt caccggtatt tccgatccgt acgaagcgcc ggagaatccc
     6541 gagatgaaga ttcagaccga ggagtgcacg cccgacgagg ctgcgcagcg catcctgctg
     6601 aagctggaaa gcctcggcta catccgctga cctcgcattg agggcaatgc cgcacgggaa
     6661 ggaagagttc tttcttcctt cctgaagtcc gagttcattc gagagattcc tcatggatcg
     6721 caaaggcctc atcctcgctg gcggctccgg cacgcggctg catccgctga cgttgtcggt
     6781 gagcaagcag ctgatgccgg tgtacgacaa gccgatgatc tactacccgc tgacgacgct
     6841 gatgctcgcc ggcatccggg agatcctgat catcacgacg ccgcgcgacc aggaggcatt
     6901 ccaggcgctg ctcggcgacg gcagccagtg gggcgtgcgg ctggaatacg ccgtgcagcc
     6961 gagtccggat ggcctcgcgc aggccttcct gatcggcgag gaattcctcg ccggcagtcc
     7021 ggcggcgctg gtgctgggcg acaacatcta tcacggccac ggcctgccgg aactgctgcg
     7081 ccgggccgat gcacgggagc agggcgcgac ggtgttcggc tactacgtgc gcgatcccga
     7141 gcgctacggc gtggtcgagt tcgatgcagc cgggcgtgcg gtgtcggtcg aggaaaaacc
     7201 ggcgcagccg aagtcgaatt acgcgatcac cggcctgtac ttctacgatg ccgatgtggt
     7261 cgaggtggca aaagccgtca gaccttcggc ccgaggtgag ctggagatca ccgatgtcaa
     7321 ccgcatctac ctggagcgcg ggcagctcaa cgtcgagctg atggggcgcg gttatgcgtg
     7381 gctcgacacc ggcacgcatg attcgctgct cgatgcggcg aacttcattc aggtcatgca
     7441 ggcccgtcag ggactgcaga tcgcctgccc cgaagaaatc gcctggcgcc tcggctggat
     7501 cgacgccgaa cacctcgagc gcatggcaaa accgctcgcc aagaacggct acggccgcta
     7561 cctgctcgat ctgctgcgac agggtgtgcg cccatgaaac tgatcgagac cgcactgccg
     7621 ggcgtactga tcctcgaacc gcaggtgcat ggtgatgccc gcggcttttt cgtcgagacc
     7681 taccgcgcca gctggttcga ggcgcatggc ctgacggcac ccgtgttcgt gcaggacaac
     7741 cagtcccgct cgcgccgcgg cgtattgcgc ggcctgcatt accagactgt caacccgcag
     7801 ggcaagctgg tgcgctgcgc gcgcggcgcg gtgttcgatg tcgccgtgga cgtgcgccgc
     7861 ggttcgccgc atttcggcca gtccgtgggc gtcgtgctcg atgatgaatc gcatcgccag
     7921 ctgtggattc cgcccgggtt tgcacacggt ttctgcgtgc tcagcgacgt ggccgacttt
     7981 gcctacaaat gcaccagtta ctacgacccg gcctccgatg ccggcatccg ctggaacgac
     8041 ccggcgctcg gcatcgactg gccgcttgag gacatcgaac cgctgctgtc agccaaggat
     8101 caactgctgc cgctgcttgc cggacaggct gacgacaagc tgaccgtata cgggggctga
     8161 accgatgagc attctcgtca ccggcgcaaa cggtcaggtc ggatatgaac tggtgcgccg
     8221 tgccggcccg ttgccggtca cgggcctcac gcgcagccag ctcgacatct gcaacgcgca
     8281 ggcggtgcag gcggccatcg gccagcatga ggctcgcatc gtcatcaacg ctgccgccta
     8341 caccgccgtc gacaaggccg aaaccgagcc gcaggccgcc ttcgcggtca atcgcgacgg
     8401 tccggccacg ctggctgccg cctgcgcggc tgccggcatc ccgctgctgc atctgtccac
     8461 cgactatgtg ttcgatggca gcaaacccgc accgtatacc gaagacgatc cggttgcacc
     8521 gctgggaacc tacggcgcca gcaagctcgc cggcgaggat gccatccgtg ccagcggctg
     8581 cgcgcatctg atcctgcgcg tcgcctgggt gttcggtgcc cacggcaaca acttcgtgcg
     8641 cacgatgctc aggctcggcc gcgagcgcga acagctgcgt gtcgtcgccg accagctcgg
     8701 cggtccgacc ccggcagccg ccattgccga cgcgctgctg caactggccg ggcgacacct
     8761 cgccggagaa gccctgccga acggaacctg gcatttcgcc ggcgtgccgc acaccagctg
     8821 gcacggcttt gccaatgccg cgctcgaagc cgctgccgcc cgcggcctgc tcgcccgcgt
     8881 gccgcgcatc gacgccatca ccagcgccga atacccgacc cccgcccggc gaccggccaa
     8941 ctcccggctc gacagcacgc gcgccgaaac cggactcggt ctcgacgttc cggactggcg
     9001 tgccggtctt gcctccatcc tcgacgaatg gaaatccgca tgagctacac cccccgcaac
     9061 gtcctggtca ccggcggtgc cggcttcatc ggcgccaact acgtacgctg gctgctcggc
     9121 gaggatgccg gggtgcacat cgtcaacctc gacgcgctga cctacgccgg cagcaccggc
     9181 aacctgaagg gtctgcccgg tgaggagcgt cataccttcg tgcagggcga catctgcgac
     9241 gccgcgctgg tcgcccggct gctgcgcgag cacgccatcg acaccgttgt gcactttgcc
     9301 gccgaatcgc acgtcgatcg ctcgatcagc ggtcccggcg agttcgtgcg caccaacgt
//
LOCUS       DAOQIE010000141         9351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3731_length_9351_cov_6.682444, whole genome shotgun sequence.
ACCESSION   DAOQIE010000141 DAOQIE010000000
VERSION     DAOQIE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_9351_cov_6.682444"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="PK144_10015"
     CDS             <1..1807
                     /locus_tag="PK144_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNF66104.1"
                     /translation="DTIATLRLAEQLGGETTTLAGRAIAEEILSHARARNVTRIVVGH
                     SQRRGWRAWFRSGLVDALLRGAGDIEIHAVPAPAATPEPPAAAAASTPLPPATPVPWR
                     GYGLAFAGVMLATAVAHLMTPRFELANLIMVYLLAVVFVARRGEREPALLAAVLAVLA
                     FDFFFVPPQLTFAVSDTQYLVTFAIMLVVGGIISQLTVREHEQTEHARLRERRTAALH
                     RLSKNLASLRGADALLDAAVRHIAEVFEAEVLALLPDDAGRLTVRAGWHASFAMDAKE
                     QSVAQWVHDLGRAAGLGTATLPYVDAVYVPLLGKRGAVGALRVKPALAERLVLPEQMR
                     LLEAFANQTALALEVDRLGEEAREAKLAAETERLRSSLLASVSHDLRTPLAAIIGSAS
                     SLAEYGDGLDAEERRELALNIHAEAERLNRLVANLLQATRLEAGTLTPLREAHDLEAV
                     IGSALERLSRALAGRPFHTDIADGLPLVSIDALLIEQVFVNLIDNALRYTPAGSPIEL
                     TVRLEDGDVRVELADRGPGLPAAALGQVFERFWRGAPDSHPGGAGLGLAICRGIVELH
                     GGRIEALNRTGGGAVFRFTLPVALPLALADAGELP"
     gene            1807..2511
                     /locus_tag="PK144_10020"
     CDS             1807..2511
                     /locus_tag="PK144_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66105.1"
                     /translation="MQPPKPVLLVVEDELAIRRFLRTALRAQGYTLFEAATGTEALTL
                     AAQERPDLILLDLGLPDLDGLDVLGRLREWSTVPVIILSARGQEADKVRGLDAGADDY
                     LAKPFGVPELLARLRVALRHRSRSGTADPVFTAGGLSVDLAAHRVTRDGSDIHLSPRQ
                     FRVLAVLVRHAGRVVTQQQLIREVWGEASDATSDSVRIAMHGLRHRLEPDPVRPRWLR
                     TEPGIGYRLVGEEIAD"
     gene            2589..2879
                     /locus_tag="PK144_10025"
     CDS             2589..2879
                     /locus_tag="PK144_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNF66106.1"
                     /translation="MSESPARSPRSARLGLRATPEQEALLKRAAEIRHKSLTDFVLDS
                     ACLAAEQTLLDQRLFRISEGEYAALLELLDRPAADNPGLQQLFSKPDPWQAG"
     gene            2879..3376
                     /locus_tag="PK144_10030"
     CDS             2879..3376
                     /locus_tag="PK144_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF66107.1"
                     /translation="MPAPPEALQAHHACTAFRCGRPALDDWLQRHALQAQGSGSARSF
                     VVCEAGRVIAYYSLTVGQIEPAAAPDRIRQGMGRFPLPVVLLARLAVDGAYQGRGIGA
                     ALLRDAIRRSLIIAEQAGVRAMLVHPLDEHAARFYDACGFVPAPFGAGHRLLLLKDAR
                     RLMAQ"
     gene            complement(3438..6182)
                     /gene="fdhF"
                     /locus_tag="PK144_10035"
     CDS             complement(3438..6182)
                     /gene="fdhF"
                     /locus_tag="PK144_10035"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNF66108.1"
                     /translation="MSADDAGPAPIPFTLDGRALDARPGETILSAAARHGIAIPYLCA
                     ADGLAPAGNCRACVVAIDGERTLAPACCRTPAAGMQVQLETERVRKVQRLVLELLAAE
                     TAGAVQKPDSELTHWQQALGVTSTRFAARMQPPADASHPAIRVALDACIQCTRCLRAC
                     RDVQVNDVIGLAARGHDTRIVFDLDDPLAASSCVACGECVQACPTGALLPAREAVSVA
                     ERVVPSVCPYCGVGCQLDYHVAGQRILRVSGRDGPANHGRLCVKGRFGFDYVSHPQRL
                     TQPLIRRHGVPKSLEGFDPERPERCFRPASWEEALDYVASRLLSLRDLHGSDALAGFG
                     SAKGSNEEAYLFQKLVRTGFGSNHVDHCTRLCHASSVAALLEGIGSGAVSNPVADVMS
                     AEAEVIVVIGANPTVNHPVAATFMKQAAKAGKTLILIDPRRTELARHARYVLQFRPDT
                     DVALLNALLHTVIDEGLTDEAFIRERTEDFERLREQVAGCSPEAMAPVCGVPAALIRE
                     VARVYARARGAMIFWGMGISQHVHGTDNARCLIALALATGQIGRPGTGLHPLRGQNNV
                     QGASDAGLIPMVFPDYRRVDDPEARARFERLWGTALDPVPGLTVVEIMHAILEGRIRG
                     MYILGENPAMSDPNLHHARAALAALEHLVVQDLFLTETAAFADVVLPASAFPEKTGTF
                     TNTDRRVQLGRAALDPPGEARQDLWIIQQIARRLGLDWDYPDARTVFDEMRQAMDSIA
                     GITWERLEAEHAVTYPCLHEGDPGEPVIFTDRFPRPSGRARFVPAALIPAAERPDADY
                     PCVLITGRQLEHWHTGSMTRRAGVLDALEPEAIVTLHPEDYAALNPGPDGLRVRLETR
                     RGQLSARARPDPALMRGQVFMAFCYVEAAANLLTVDALDPYGKIPEFKYCAVRVVRAG
                     "
     gene            complement(6179..7891)
                     /locus_tag="PK144_10040"
     CDS             complement(6179..7891)
                     /locus_tag="PK144_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNF66109.1"
                     /translation="MNGRRSGRGGPKGRQVDAQALAEVRETIGELPRERSLLIEHLHA
                     LQDRFRHLAPAHLVALAAELGLAPVEVYEVASFYHHFDVLADGDPPPPGLTVRVCTSL
                     SCELAGAPALAEAIEAAVHAGVRVQRVPCVGRCDVAPLAIVGRNHVPWATVAKVMVRV
                     DDARTEPEHRPVWIDLDGYRAHGGYRLLCDCVEGRRSLDELLATLDAAALRGLGGAGF
                     PAGRKWRTVRSFPGPRCMAVNLDEGEPGTFKDRFWLEQDPHRFFEGLLLAAWAVGADE
                     IYVYTRDEYPVCRRVLAEAHAELLANPPSPCALPVIHFRRGAGAYICGEESAMLESIE
                     GRRGLPRLRPPYVAERGLHGRPTLVQNFETLYWLRDILERGAGWWNGHGRHGRRGLRS
                     FSVSGRVREPGVKLAPAGITLHELIDEYCGGMLPGHALHAYFPGGASGGILPARLAGL
                     PLDFDTLQPYGCFIGSAAVIVLSDQDSVRAAALNAMRFFADESCGQCTPCRVGTAQAA
                     QLMAAPRWDTALLADLAVVLREASICGLGQAAPNTFECALRYFPEEFPELSREARTPG
                     DPAP"
     gene            8079..8681
                     /locus_tag="PK144_10045"
     CDS             8079..8681
                     /locus_tag="PK144_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66110.1"
                     /translation="MKVDKIRTLKAELKRAFALRLDAVKDARSLNELQRSLADDTTFR
                     DAMTRLMASEDPGMSLTQEPQLLHRLMEKGAARWRELIAAGGGACTVEEVARVTGMSA
                     DAVRKAAQRRQLLAYREGTRLMFPACQFEHGRVLDGLRELAMQLPPGLSDRAVVSFFL
                     SPPGTQESARSPIERLRAGDPAEIEQARQAARLYLEQAAT"
     gene            8678..9289
                     /locus_tag="PK144_10050"
     CDS             8678..9289
                     /locus_tag="PK144_10050"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNF66111.1"
                     /translation="MSRLPEVTDDENSLPCRSLPAGSRWHRIARLAHASPLYFDRAAG
                     SRFNSGSATFGTLYLAETLTGALAESLCRNAALRLRCHRHTPLPELAARGDYLVTIGV
                     PMRVLDLTVPNLVRHGLTADLYGEYDARRPVPYRWGPAWADHVHGLGLDGILYPSRHH
                     TACICLAVFERSITDFRHALVAPLDRHPEALRVLDEVFDWAIV"
BASE COUNT         1332 a         3260 c         3386 g         1373 t
ORIGIN      
        1 cgacacgatc gcgacgctcc ggctggccga acagctcggc ggcgaaacca cgacgcttgc
       61 cggccgcgcg atcgccgagg aaatcctgag ccatgcgcgc gcgcgcaacg tcacgcgcat
      121 cgtcgtcggc cactcgcagc ggcgcggctg gcgggcgtgg ttccgttcgg ggctggtcga
      181 tgcgctgctg cgcggggccg gcgacatcga gatccacgcg gtgccggccc cggccgcaac
      241 gccggaacca ccggctgcgg ccgccgccag cacgccgctg ccgcccgcga cgccggtgcc
      301 gtggcgcggc tacgggctgg ccttcgccgg cgtcatgctc gcgaccgcgg tcgcgcacct
      361 gatgacgccg cgcttcgagc tggcgaacct gatcatggtc tacctgctcg cggtcgtgtt
      421 cgtcgcgcgg cgcggcgagc gcgagccggc gctgctcgcg gcggtgctgg cggtgctcgc
      481 attcgacttc ttcttcgtgc cgccgcagct gaccttcgcg gtctccgaca cgcagtacct
      541 cgtcaccttc gcgatcatgc tcgtcgtcgg cggcatcatc agccagctca ccgtgcgcga
      601 gcacgagcag accgagcacg cgcggctgcg cgagcggcgt accgcggcgc tgcaccggct
      661 gtcgaagaac ctcgccagcc tgcgcggggc cgatgcgctg ctcgatgccg ccgtgcgcca
      721 catcgccgag gtgttcgagg ccgaggtgct ggcgctgctg cccgatgacg ccgggcgcct
      781 caccgtgcgt gccggctggc acgcgagctt cgcgatggat gccaaggagc agagcgtcgc
      841 gcagtgggtg cacgacctcg gtcgcgccgc cgggctcggc accgcgacgc tgccttacgt
      901 cgatgccgtc tacgtgccgc tgctcggcaa gcgcggcgcg gtcggggcgc tgcgcgtgaa
      961 gccggcgctc gccgaacgcc tggtgctgcc cgagcagatg cgcctgctcg aagccttcgc
     1021 gaaccagacc gcgctcgcgc tcgaggtcga ccggctcggc gaggaggcgc gcgaggcgaa
     1081 gctcgcggcc gagaccgagc gcctgcgcag ctcgctgctg gcgtcggtgt cgcacgacct
     1141 gcgcacgccg ctcgcggcga tcatcggctc ggcatcgagc ctcgccgaat acggcgacgg
     1201 gctcgacgcc gaagagcgcc gggagctcgc gctgaacatc cacgccgagg ccgagcgcct
     1261 gaaccggctg gtcgccaacc tgctgcaggc cacgcgcctg gaggccggca cgctgacgcc
     1321 gctgcgcgag gcccatgatc tcgaagccgt gatcggcagt gcgctggagc gcctgtcgcg
     1381 ggcgctggcc gggcggccgt tccataccga catcgccgat ggtctgccgc tggtgtcgat
     1441 cgacgcgctg ctgatcgagc aggtgttcgt gaacctgatc gacaacgcgc tgcgctacac
     1501 gccggccggc agtccgatcg agctgacggt gcggctggag gatggcgacg tgcgggtcga
     1561 actcgccgac cgcggcccgg gcctgccggc ggccgcgctc ggacaggtgt tcgagcgctt
     1621 ctggcgcggc gcgccggaca gccacccggg cggcgccggg ctgggactcg cgatctgtcg
     1681 cggcatcgtc gagctgcacg gcggccgcat cgaggcgctg aaccggaccg gcggcggtgc
     1741 ggtgttccgc ttcacgctgc cggtggcctt gccgctcgcg ctggccgatg ccggggaact
     1801 gccgtgatcc agccgccgaa gccggtgctg ctcgtcgtcg aggacgaact ggcgatccgc
     1861 cgcttcctgc gcacggcgct gcgcgcgcag ggctacacgc tgttcgaggc cgcgaccggc
     1921 accgaggcgc tgacgctcgc cgcccaggag cgccccgacc tgatcctgct cgatctcggc
     1981 ctgcccgacc tcgacggcct cgacgtgctc gggcgcctgc gtgaatggag cacggtgccg
     2041 gtcatcatcc tgtcggcgcg cggtcaggag gctgacaagg tgcgcggcct cgacgccggc
     2101 gccgacgatt acctcgccaa gcccttcggc gtgcccgagc tgctggcgcg gctgcgcgtg
     2161 gcgctgcgcc accgcagccg cagcggcacg gccgatccgg tgttcacggc cggcggcctg
     2221 agcgtcgatc tcgccgcgca tcgcgtcacg cgcgacggca gcgacatcca cctgagcccg
     2281 cggcagttcc gcgtgctcgc cgtgctcgtg cgccacgccg ggcgcgtggt cacgcagcag
     2341 cagctgatcc gcgaggtctg gggcgaggcc tcagacgcca cttccgattc cgtgcgcatc
     2401 gcgatgcacg ggctgcggca ccgcctggag ccggacccgg tgcggccgcg ctggctcagg
     2461 acggagccgg ggatcggata ccggctggtc ggggaggaaa tcgccgactg aagcaggcag
     2521 ggcagcacaa ggatcgtcgt acgcctgtcg tgctaacgtc tgcgcattcc cgtagccgga
     2581 gcgccaccat gtccgaatct cctgcacgtt cgccccgcag cgcccggctg ggtctgcgtg
     2641 ccacccccga gcaggaagcc ctgctgaagc gtgccgccga aatccggcac aagtcactga
     2701 ccgatttcgt gctcgacagc gcctgcctgg cggccgagca gaccttgctg gatcagcggc
     2761 tgttccggat cagcgagggg gaatacgccg cgctgctcga actgcttgac cgcccggccg
     2821 cggacaatcc cggcctgcag caactgttct cgaagcccga tccctggcag gcgggctgat
     2881 gccggcgcca cccgaggccc tgcaggccca tcacgcatgc acagcattcc gctgcggccg
     2941 gcccgcgctc gatgactggc tgcagcggca tgcgctgcag gcgcagggca gcgggtcggc
     3001 gcgaagcttc gtcgtctgtg aggccggacg ggtgattgcc tactacagcc tgacggttgg
     3061 ccagatcgag ccggccgctg caccggatcg catccgtcag ggcatgggcc gctttccgct
     3121 gccggtcgtg ctgctggcgc gactggcggt cgatggtgcg tatcaggggc gtggcatcgg
     3181 cgccgccctg ctgcgcgatg cgatccgccg ttcactgatc attgccgagc aggccggagt
     3241 gcgggcgatg ctggtgcatc cgctcgatga gcacgctgcg cggttctacg acgcttgcgg
     3301 tttcgtgccg gcaccattcg gagcgggcca ccggctgttg ctgctgaagg atgccaggcg
     3361 gctcatggcg caatgaccac atctgctgcg tggttgttat acagcccggg attctgttta
     3421 acagctgccg tccgccctca gcccgcgcgc acgacccgca ccgcgcagta cttgaactcc
     3481 ggaatcttcc cgtacgggtc gagcgcgtcg acggtcagca ggttggcggc ggcctcgaca
     3541 tagcagaagg ccatgaacac ctggccgcgc atcagcgccg ggtcggggcg ggcgcgggcg
     3601 ctgagctggc cgcggcgggt ttcgaggcgc acccgcaggc cgtcggggcc ggggttcagc
     3661 gcggcgtagt cctcggggtg cagcgtcacg atggcttcgg gttcgagtgc atcgagcacg
     3721 ccggcgcggc gcgtcatgct gccggtatgc cagtgttcga gctggcgacc ggtgatcagc
     3781 acgcacgggt agtcggcatc cgggcgctcg gcggccggga tcagcgccgc cggcacgaag
     3841 cgcgcacggc cggacggacg cggaaaacgg tccgtgaaga tcaccggctc gcccggatcg
     3901 ccttcgtgca ggcacggata cgtgaccgcg tgttcggctt cgagccgttc ccaggtgatg
     3961 ccggcgatgc tgtccatcgc ctggcgcatc tcgtcgaaca ccgtgcgcgc gtccgggtaa
     4021 tcccagtcca gaccgagccg gcgggcgatc tgctggatga tccagaggtc ctggcgagcc
     4081 tcgccgggcg ggtcgagcgc ggcgcggccg agctgcacgc gccggtcggt gttcgtgaag
     4141 gtgccggtct tctccgggaa ggcgctcgcc ggcagcacga catcggcaaa tgccgcggtc
     4201 tcggtcagga acaggtcctg cacgaccaga tgttcgagcg cggcgagcgc ggcgcgggcg
     4261 tggtgcaggt tcgggtccga catcgccggg ttctcgccga ggatgtacat gccgcgaatc
     4321 cggccttcga ggatcgcgtg catgatctcg acgacggtca ggcccggcac cggatcgagc
     4381 gccgtgcccc acagccgctc gaagcgcgcc cgggcttcgg ggtcgtcgac gcgccggtag
     4441 tccgggaaca ccatcggaat aaggccagcg tccgaggccc cctgcacgtt gttctggccg
     4501 cgcagcggat gcaggccggt gccgggccgg ccgatctggc cggtcgcgag cgcgagcgcg
     4561 atcaggcagc gggcgttgtc ggtgccgtgc acgtgctggc tgatgcccat gccccagaag
     4621 atcatcgcgc cgcgcgcccg cgcatagacg cgcgcgacct cgcggatcag cgccgccggc
     4681 acgccgcaga ccggcgccat cgcctcgggg ctgcagccgg cgacctgctc gcgcagccgc
     4741 tcgaagtcct cggtgcgctc gcggatgaag gcttcgtcgg tcaggccctc gtcgatgacg
     4801 gtgtgcagca gcgcgttcag cagcgccacg tcggtgtcgg gccggaactg cagcacgtag
     4861 cgggcgtggc gcgcgagttc ggtgcgccgc ggatcgatca ggatcagcgt cttgccggct
     4921 ttcgcggcct gcttcatgaa cgtcgccgcg accggatgat tgacggtcgg attggcgccg
     4981 atgacgacga tgacctcggc ctcggcgctc atgacatcgg cgaccgggtt cgacaccgcg
     5041 cccgagccga tgccttcgag cagcgccgcg accgacgagg catggcagag ccgcgtgcag
     5101 tgatcgacgt ggttcgagcc gaagccggtg cgcaccagct tctggaacag gtaggcctcc
     5161 tcgttgctgc ccttggccga gccgaagccg gcgagcgcat cggacccgtg caggtcgcgg
     5221 agcgacagca ggcggctcgc aacgtaatcg agcgcctcct cccagctcgc cggccggaag
     5281 cagcgttcgg ggcgctcggg atcaaagcct tcgagcgact tcggcacgcc gtggcggcgg
     5341 atcagcggct gcgtcaggcg ctgcgggtgg ctcacgtagt cgaagccgaa gcggcccttg
     5401 acgcacagcc ggccgtggtt ggccgggccg tcgcggccgc tgacgcgcag gatgcgctgg
     5461 ccggcgacgt ggtaatcgag ctggcagccg acgccgcagt acgggcagac cgagggcacg
     5521 acgcgctcgg cgacgctgac ggcttcgcgc gccggcagca gcgcgccggt cgggcaggcc
     5581 tgcacgcact cgccgcaggc gacgcaggaa ctcgccgcca gcgggtcgtc gaggtcgaag
     5641 acgatgcgcg tgtcatgacc acgggcggcg aggccgatca cgtcgttgac ctgcacgtcg
     5701 cggcaggcgc gcaggcagcg cgtgcactgg atgcaggcat cgagcgcgac gcggatcgcc
     5761 ggatgcgagg catcggccgg cggctgcatg cgcgcggcga agcgtgtcga cgtgacgccg
     5821 agcgcctgct gccagtgcgt cagttcggag tcgggcttct gcacggcgcc ggcggtttcg
     5881 gcggcgagca gttcgagcac gaggcgctgg accttgcgca cgcgttcggt ttcgagctgc
     5941 acctgcatgc cggccgccgg cgtgcggcag caggccggtg ccagcgtgcg ctcgccgtcg
     6001 atcgcgacca cgcaggcgcg gcagttgccg gccggtgcga gaccgtcggc ggcacagaga
     6061 tacgggatcg cgatgccgtg gcgcgccgcg gcgctgagga tcgtctcgcc ggggcgggcg
     6121 tcgagcgcgc ggccgtcgag cgtgaacggg atcggggcgg ggccggcgtc atcggcgctc
     6181 acggggcggg gtctccgggc gtgcgcgctt cccgggacag ctcgggaaac tcttccggga
     6241 agtagcgcag cgcgcattcg aaggtgttcg gtgccgcctg gccgaggccg cagatcgagg
     6301 cctcgcgcag gacgacggcg agatcggcga gcagcgccgt gtcccagcgc ggcgcggcca
     6361 tcagctgcgc ggcctgcgcg gtgccgacgc gacacggcgt gcactggccg caggattcgt
     6421 cggcgaagaa gcgcatcgcg ttcagcgccg cggcgcgcac cgaatcctga tccgacagca
     6481 cgatgaccgc cgccgagccg atgaagcagc cgtagggctg cagcgtgtcg aaatcgagcg
     6541 gcaggccggc gaggcgcgcc ggcaggatgc cgcccgaggc gccgcccggg aaatacgcat
     6601 gcagcgcgtg gccgggcagc atgccgccgc agtattcgtc gatcagctcg tgcagcgtga
     6661 tgccggccgg cgcgagcttg acgcccggct cgcgcacccg gccgctgacc gagaacgagc
     6721 gcaggccccg gcggccgtgc cggccatgac cgttccacca cccggcgccg cgttcgagga
     6781 tgtcgcgcag ccagtacagc gtctcgaagt tctgcaccag cgtcgggcgg ccgtgcagcc
     6841 cgcgctcggc gacatacggc ggacgcagcc gcggcaggcc gcgcctgccc tcgatcgatt
     6901 cgagcatcgc cgattcctcg ccgcagatgt aggcgccggc gccgcggcgg aagtggatca
     6961 ccggcagcgc gcagggcgag ggtggatttg cgagcagttc ggcatgggcc tctgcgagca
     7021 cgcgccggca caccggatac tcgtcgcggg tatagacgta gatctcgtcc gccccgaccg
     7081 cccaggctgc gagcagcagg ccctcgaaga agcggtgcgg gtcctgctcc agccagaagc
     7141 ggtccttgaa ggtgccgggt tcgccttcgt cgaggttgac ggccatgcac cgcggccccg
     7201 gaaagctgcg cacggtccgc cacttgcgcc cggccgggaa gcccgccccg ccgagcccgc
     7261 gcagcgccgc ggcatcgagc gtcgccagca gctcatcgag gctgcgccgg ccctcgacgc
     7321 agtcgcagag cagccggtag ccgccgtggg cgcggtagcc gtcgaggtcg atccataccg
     7381 gccggtgttc gggctcggtg cgggcatcgt cgacgcgcac catgaccttg gccacggtcg
     7441 cccacggcac gtggttgcgc ccgacgatcg caagcggcgc cacgtcgcag cgcccgacgc
     7501 agggcacgcg ctgcacgcgc acgccggcat gcacggcggc ttcgatcgcc tcggccagcg
     7561 ccggcgcacc ggccagttcg cagctcagcg aggtgcacac gcgcaccgtc agccccggcg
     7621 gcggcgggtc gccgtcggcg agcacgtcga agtggtgata gaagctcgcg acctcgtaca
     7681 cctcgaccgg cgccagcccg agttcggccg cgagtgccac gagatgcgcg ggcgccagat
     7741 gccggaagcg gtcctgcagc gcgtgcaggt gctcgatcag cagcgagcgc tcgcgcggca
     7801 gctccccgat cgtctcgcgc acctcggcca gtgcctgcgc atcgacctgc cggcccttcg
     7861 gcccgccgcg tccgctccgt cgcccgttca tgatcgtctg gcctcgtcgg gatgccggaa
     7921 caagtgggca agcgtaggtg ctcgaccgga cagtcgccca ggcaggcgtg caggtatcca
     7981 tggcgcccgg gcttgtcatg cctgtccatc ctgtccatat tgtccaggct gtcccgcaag
     8041 aatcggttga aaccgtggct gactgatcct gaggctggat gaaggtggac aagattcgca
     8101 cgctgaaagc cgaactgaag cgcgcttttg cgctgcggct cgacgctgtc aaagacgccc
     8161 ggtccctgaa cgaactgcag cgctcgctgg ccgatgacac gactttccgc gatgccatga
     8221 ctcgcctgat ggcatccgaa gaccccggaa tgtccctgac tcaggaaccg cagctgctgc
     8281 accggttgat ggagaaaggt gcagcgcgct ggcgtgaact gattgcagcg ggcggcggtg
     8341 cctgcacggt cgaagaggtt gcgcgtgtga ccggcatgag cgccgatgcc gtgcgcaagg
     8401 cggcccagcg caggcagttg ctggcttatc gggagggaac ccggctgatg tttccggcct
     8461 gtcagttcga gcatggccgt gtgctcgacg gcctgcgcga actggccatg cagttgccgc
     8521 cgggcctgtc ggatcgcgcc gtcgtgagct tcttcctgtc gccgcccgga acgcaggaaa
     8581 gtgccagatc gccgatcgaa cggctgcgcg ccggtgatcc ggcggaaatc gaacaggccc
     8641 gtcaggccgc ccggctgtat ctggagcagg ccgcgacgtg agccgcttgc cggaagtcac
     8701 ggacgatgaa aacagcttgc cctgccgttc cttgccggcc ggcagccgct ggcaccggat
     8761 cgcaaggctg gcgcatgcat caccgctgta tttcgaccgg gcagcgggca gccgcttcaa
     8821 ttcaggcagt gccaccttcg gcacgctgta tctggcagaa acactgacgg gggcgctcgc
     8881 cgaaagcctc tgccgcaatg ctgccctgag gctgcgttgt catcggcata cgccgctgcc
     8941 ggaactcgcg gcccggggcg attaccttgt cacgatcggt gtaccgatgc gggtgctgga
     9001 tctgacggtt ccgaatctgg tgcgtcacgg cctgacggcc gacctctacg gcgaatacga
     9061 tgcccgccgg ccagtaccgt atcgctgggg gccggcttgg gccgatcacg ttcacggtct
     9121 cggactggac ggcatcctgt acccgtcccg gcatcacacg gcctgcatct gtctggcggt
     9181 gttcgagcga agcatcaccg acttcaggca tgccctggtc gcaccgctcg atcgccaccc
     9241 ggaagccttg cgcgtgctgg acgaggtatt cgactgggcc attgtctgac gggcccggct
     9301 caccccgccg ccggcagccg caccgtgaac accgccccgc ccgccgcgcc g
//
LOCUS       DAOQIE010000142         9299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3756_length_9299_cov_8.577888, whole genome shotgun sequence.
ACCESSION   DAOQIE010000142 DAOQIE010000000
VERSION     DAOQIE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3756_length_9299_cov_8.577888"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1472)
                     /locus_tag="PK144_10055"
     CDS             complement(24..1472)
                     /locus_tag="PK144_10055"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNF66112.1"
                     /translation="MSQAQHDLILQDWKNRQEIAEEMLPLLGRLYRDRGLVLLVYGRP
                     IVNSSTIEILKAHRYVRYHEGAELDVADTLAVLKAIAGMDVAPARLDIGKLAVGFRKA
                     GTAVSLADYLGEQLADIVGDHAQKSLEPKDVVLFGFGRIGRLLARLLIERTGAGNKLR
                     LRAIVVRKGKGADLQKRASLLRRDSVHGPFNGSITVDEENNAIIANGNFIKVIYADKP
                     GAVDLTEYEINDALIVDNTGKWADLEGLTQHLSGKGAKKVLLTAPSKGDGIKNIVYGV
                     NHGTIEDSDLVVSAASCTTNAITPVLKALNDKYGIENGHVETVHSYTNDQNLIDNYHK
                     SDRRGRAAPLNMVITSTGAAKAVAKALPELKGKLTGNAIRVPTPNVSMAILSLNLQSE
                     VTKDGINEFLRETALHSKLSDQIDYTASTELVSTDIVGSRHAGVVDSQATIAADKRVV
                     LYVWYDNEFGYSCQVVRVMQEMVGIRLPNLPH"
     gene            1773..3347
                     /gene="gshA"
                     /locus_tag="PK144_10060"
     CDS             1773..3347
                     /gene="gshA"
                     /locus_tag="PK144_10060"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNF66113.1"
                     /translation="MYRSAQARLTRLLDAAGPVAVRGCLKGLEREALRVAPDGRIAQT
                     RHPRALGAALTHPWITTDYSEALLEFITPPVADTQDCTRFLDEVHRFVLPRLGDEKIW
                     PGSMPCVVDGEDGIPIAEYGSSNAGYMKHVYRRGLGYRYGKLMQVIAGVHFNFSLPEA
                     FWAALREIDGSIAAQPAYVSDGYFRLTRNVQRVGWLVPYLFGASPAVCRSFIGARGED
                     LDEWDDGTLYLPYATSLRMSGIGYQNRKEKKCGVHVRYDGLDAYVASLARAVNTPCPE
                     YEAIGVCRAGDWRQLSGNILQIENEYYSTVRPKQPPLGNEKVTAALARRGVRYVELRS
                     LDVDPYQPTGVDAVTLRFVEALLIFCTLADSPRIELQEQLDVNANLERVARAGRDPGL
                     LLARRGASIGLRDWGLGILAALEPVCAVLDGADPESPYTAALNEQRGKLEDPARTPSA
                     RVLAEMGEREEGFFDFGWRLATEHAATLRRRPLRPAREAEFRVLAERSHAEQAAMETA
                     DDIGFEEYLRRYFAQR"
     gene            complement(3361..3507)
                     /locus_tag="PK144_10065"
     CDS             complement(3361..3507)
                     /locus_tag="PK144_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66114.1"
                     /translation="MRACIGQGLRADLERLDAEPVSRLLAALRAEGVPEETLAKAVEA
                     LTTA"
     gene            complement(3585..5918)
                     /locus_tag="PK144_10070"
     CDS             complement(3585..5918)
                     /locus_tag="PK144_10070"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF66115.1"
                     /translation="MTLRRLRIGTRLALGFGAILVIVAATLTAAMLHDLAGIQTASRA
                     ERLNGSIGLVIASMQREQRAAEALATLVASQPSVVEAFAAGDRERLLAEFGGAFAALK
                     TGQNLEQFQFHGPGAISFLRVHQPQQHGDDLSATRRTIVDAQQSGKAVAGLEADAGGF
                     GLRAVVPIRRGEQLLGTVEFGVAMGQGFFDELMRWYLLRARLVTLDDAGQLRRLADTA
                     GNWPDLGEAELRAAAKGTPAMGDIITGDAHLAVLIMPIADYAGRTAGALEVFQDQTLF
                     EQTARQIRQTALALGAGLLLLGCLLAWWLARGIARPLGAMAEAAQAIAAGDTSCEVRY
                     VGRDEVGRLADAFRAMLAYLRELTQAVDALDRGDTARRHTARSEADRLGTSVARTQAT
                     VAELLAETQSLVHAAEVGELARRGRTERFSGDYGALVASVNHLLDTRAAARERETAAE
                     AERLAAARAATEQLATQVETLLARVDAAARGDLTVQVDGSDEDAIGRVGAGLSRLLGD
                     LRGHLGAIGSAAQALGTRAADLDALARRLRGDADGHRTRTADAAADAEDVGRRLDAVA
                     AATEQMSATAREIAHNTEQASGIGSEAERAAADAETLMQRLAESGNQIGDVLRLITSI
                     AEQTNLLALNATIEAARAGEAGKGFAVVAGEVKELARATAEATGRIHERIDNIQRDTA
                     QAQTAIARTRDIIGRVNAFEQSIAQAAEQQSLAAAEMAGHVAEAAQRGQHIAQVVREL
                     AAGADGTVHASAEVLDSARALSETAGTLTERVGQFRT"
     gene            6316..8976
                     /gene="aceE"
                     /locus_tag="PK144_10075"
     CDS             6316..8976
                     /gene="aceE"
                     /locus_tag="PK144_10075"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNF66116.1"
                     /translation="MTAEQYKDVDPQETREWLAALDGVIAHVGAERAHWLIEELIERT
                     RLAGDLAPFSANTPYVNTVPVEKQAPIPGDQDIEHRIRSFVRWNAAMMVLRANRETNV
                     GGHIASFASAATLYEVGYNHFWRAPSDGHNGDLLFIQGHSSPGIYSRAFLLGRLSAEQ
                     MDNYRQETDGKGLSSYPHPWLMPDFWQFPTVSMGLGPLLAIYQARFMRYLEDRGHGVH
                     NNRKVWAFLGDGETDEPESLGAIGMAGREKLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEAEFRGSGWNVIKIVWGSKWDALLARDKSGILMKRMMEIVDGEYQTMKAKDGAYVRE
                     SFFNTPELKALVADWSDDDIWQLNRGGHDPHKVYAGFKAAVDHKGQPTVILAKTIKGY
                     GMGEAGEAANIAHQAKKMKFDDLKGFRDRFKIPVSDEQIPEYPYLTFAEGSKELDYMR
                     ARRMELGGYRPGRHPSALQLPVPGLAAFDALLKATGEGREISTTMAFVRILNTLVKDR
                     NVGKYVVPIVPDESRTFGMEGMFRQIGIWSQVGQQYTPQDADQLMFYKESKDGQILQE
                     GINEAGAMADWISAATSYAVHGVPTIPVYIFYSMFGFQRVGDLAWLAGDMRARGFLVG
                     GTAGRTTLNGEGLQHEDGHSHLWSAAVPNCISYDPTFAYELAVIVQSGLKRMYQDHED
                     IYYYLTVMNENYEHPEMPAGAEADIIKGMYAFRKGGESKGPRVQLLGSGTIFREVIAA
                     AELLRDEWGVESDIWGCPSFTELSRNGNDTVRWNLLHPTEAPQKSHVEQCLEGTEGPV
                     IAATDYMRLFSEQIRAFVPRRYVTLGTDGFGRSDTREKLRNFFEVDRRWVTVAALKAL
                     ADEGTIERSKVAEAIAKYGIDPSKPNPRLI"
     gene            9154..>9299
                     /locus_tag="PK144_10080"
     CDS             9154..>9299
                     /locus_tag="PK144_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain alpha-keto acid dehydrogenase
                     subunit E2"
                     /protein_id="HNF66117.1"
                     /translation="MSNLIEVKVPDIGDFKDVDVIEVSVQPGDTVKVDQTLIVLESDK
                     ASMDV"
BASE COUNT         1419 a         3125 c         3282 g         1473 t
ORIGIN      
        1 caggatgcgg accggggttc agttcagtgc ggcaggttcg gcaggcggat gccgaccatc
       61 tcctgcatca cgcgcacgac ctggcagctg tagccgaact cgttgtcgta ccagacgtag
      121 agcacgacgc gcttgtcggc ggcgatggtc gcctgcgagt cgacgacgcc ggcgtggcgc
      181 gagccgacga tgtcggtcga gaccagctcg gtcgaggcgg tgtagtcgat ctggtcggac
      241 agcttgctgt gcagcgccgt ctcgcgcagg aactcgttga tgccgtcctt ggtgacttcg
      301 gactgcaggt tcagcgacag gatcgccatc gacacgttcg gcgtcggcac gcggatggcg
      361 ttgccggtca gcttgccctt gagttccggc agcgccttgg cgacggcctt ggcggcaccg
      421 gtcgaggtga tgaccatgtt cagcggcgcg gcgcggccgc ggcgatcgga cttgtggtag
      481 ttgtcgatca ggttctggtc gttggtgtac gagtgcacgg tctcgacgtg accgttctcg
      541 atgccgtact tgtcgttcag cgccttcagc accggggtga tggcgttggt cgtgcacgag
      601 gcggccgaga cgaccaggtc gctgtcctcg atcgtgccgt ggttgacgcc gtagacgatg
      661 ttcttgatgc cgtcgccctt ggacggagcg gtcagcagga ccttcttcgc gcccttgccg
      721 ctcaggtgct gggtcaggcc ttcgaggtcg gcccacttgc cggtgttgtc gacgatcagc
      781 gcatcgttga tctcgtattc ggtcaggtcg acggcaccgg gcttgtcggc atagatgacc
      841 ttgatgaagt tgccgttggc gatgatggcg ttgttttctt catcgaccgt gatcgagccg
      901 ttgaacgggc cgtgcacgga gtcgcgacgc agcaggctgg cgcgcttctg caggtcggcg
      961 cccttgccct tgcgcacgac gatcgcgcgc aggcgcagct tgttgccggc gccggtgcgc
     1021 tcgatcagca ggcgggccag caggcggccg atgcggccga agccgaacag cacgacatcc
     1081 ttcggttcca gcgacttctg cgcgtgatcg ccgacgatgt cggcgagctg ttcgccgagg
     1141 tagtccgcga gcgagacagc ggtgccggcc ttgcggaagc cgacggcgag cttgccgatg
     1201 tcgaggcggg ccggggccac gtccatgccg gcgatggcct tcagcacggc cagcgtgtcg
     1261 gcgacatcga gctcggcgcc ttcgtggtag cggacgtagc ggtgggcctt gaggatttcg
     1321 atcgtggagc tgttgacgat cggacgaccg tagaccagca ggaccaggcc gcggtcgcga
     1381 tagagccggc cgagcagcgg cagcatctct tcggcgattt cctgacggtt cttccagtcc
     1441 tgcagaatca gatcgtgctg tgcttggctc attgttcgct ttccgttggg catgggttgc
     1501 tgctcgctcg atcgttccgc atgtcgaaaa atcctgcaga aacagtcgca agcgggcgcg
     1561 gattgtaatg atcgggctcc ctgcacggca agcacgcagg tcaaccaaac cgcgtcctca
     1621 cggtcaagct gcgtgtcggc ggcgtgctgg ctgcagagcg gcgatgcatg ccggcggcgg
     1681 ggtgctgcgg atgtgcgcgc tatcatgcgc gtcctgacgc ggatgaccgg cccggaccgc
     1741 cgcgttccgt gcccccagcg agtgatccct ccgtgtaccg atccgcccaa gcccgtctga
     1801 cccgcctcct cgatgccgcc ggtccggtgg ccgtgcgcgg ctgcctgaag gggctcgaac
     1861 gcgaggcgct gcgcgttgcg ccggatggcc gcatcgcgca gacccggcac ccgcgcgcgc
     1921 tcggcgcggc gctgacgcat ccgtggatca cgaccgacta ttccgaggcg ctgctggagt
     1981 tcatcacgcc gccggtcgcc gatacgcagg actgcacgcg ctttctcgac gaggtgcatc
     2041 gcttcgtgct gccgcgcctc ggcgacgaga agatctggcc gggcagcatg ccctgcgtcg
     2101 tcgatggcga ggacggcatc ccgatcgccg agtacggcag ctccaatgcc ggctacatga
     2161 agcacgtcta ccgccgcggg ctcggctacc gctacggcaa gctgatgcag gtgatcgccg
     2221 gcgtgcattt caacttctcg ctgccggagg ccttctgggc ggcgctgcgc gagatcgacg
     2281 gcagcatcgc cgcgcagccg gcctatgtgt cggacggcta cttccggctg acgcgcaacg
     2341 tgcagcgtgt cggctggctg gtgccctacc tgttcggcgc ttcgccggcc gtgtgccgct
     2401 cgttcatcgg cgcgcgcggc gaggatctcg acgaatggga cgacggcacg ctgtacctgc
     2461 cgtacgcgac ctcgctgcgc atgagcggca tcggctacca gaaccgcaag gagaagaagt
     2521 gcggcgtgca cgtgcgctac gacggcctcg atgcctacgt cgcgagcctc gcgcgcgccg
     2581 tcaacacgcc ctgtcccgag tacgaggcga tcggggtctg ccgggccggc gactggcggc
     2641 agctgagcgg caacatcctg cagatcgaga acgagtacta cagcaccgtg cggcccaagc
     2701 agccgccgct cggcaacgag aaggtcacgg cggcgctggc gcgccgcggc gtgcgctacg
     2761 tcgagctgcg ctcgctcgat gtcgatccgt accagccgac cggcgtcgat gcggtgacgc
     2821 tgcgcttcgt cgaggcgctg ctgatcttct gcacgctggc cgacagtccg cgcatcgagc
     2881 tgcaggagca gctcgatgtg aatgccaacc tcgaacgcgt ggcacgcgcc gggcgtgatc
     2941 ccggcctgct gctggcgcgc cgcggcgcca gcatcggcct gcgcgactgg gggctcggca
     3001 tccttgcggc gctggaaccg gtgtgtgcgg tgctcgacgg cgccgatccg gaatcgccgt
     3061 acacggcggc gctgaacgag cagcgcggca agctcgaaga cccggcgcgc acgccgtcgg
     3121 cgcgcgtact cgcggagatg ggcgagcgcg aggagggttt cttcgacttc ggctggcgcc
     3181 tggccaccga acacgccgcg acgctgcgcc gccggccgct gcggccggcg cgcgaggccg
     3241 agttccgcgt gctcgccgag cgctcgcacg ccgagcaggc ggcgatggag accgccgacg
     3301 acatcggctt cgaggaatac ctgcgccggt atttcgcgca gcgctgaggc ggccggcggt
     3361 tcaggcggtc gtcagggcct cgaccgcttt cgccagcgtt tcctcgggga cgccctcggc
     3421 ccgcagcgct gccagcagcc ggctgaccgg ttcggcatcg agccgctcca gatcggcgcg
     3481 caggccctgc ccgatgcagg cgcgcatcag gggctggcag ccggaatggc cgggcagggg
     3541 gggcgatgcg cttccgccat ccgcccacct cttgcagcgc ccgctcaggt cctgaactgt
     3601 ccgacccgct cggtcagcgt gccggcggtt tcgctcagtg cgcgggcgga gtcgaggacc
     3661 tcggcgctgg cgtgcaccgt gccgtcggcg ccggcggcga gttcgcgcac gacctgggcg
     3721 atgtgctggc cgcgctgcgc agcctcggcg acgtggccgg ccatctcggc ggcggcgagc
     3781 gactgctgtt cggcggcctg cgcgatcgac tgctcgaagg cgttgacgcg accgatgatg
     3841 tcgcgggtgc gggcgatggc ggtctgggcc tgcgcggtgt cgcgctggat gttgtcgatg
     3901 cgctcgtgga tgcggccggt ggcctcggcg gtggctctcg ccagttcctt gacctcgccg
     3961 gcgaccacgg cgaaaccctt gccggcctcg ccggcgcgtg cggcctcgat ggtggcgttc
     4021 agcgccagca gattggtctg ctcggcgatc gacgtgatca ggcgcagcac gtcgccgatc
     4081 tggttgccgg attcggcgag gcgctgcatc agggtttcgg catcggccgc cgcgcgttcg
     4141 gcctcgctgc cgatgccgct ggcctgctcg gtgttgtgtg cgatctcgcg cgcggtcgcg
     4201 ctcatctgct cggtggcggc ggcgacggca tcgagccggc ggccgacgtc ctcggcatcg
     4261 gcggcggcat cggcggtgcg ggtgcggtgg ccgtcggcat cgccgcgcag ccggcgggcc
     4321 agcgcatcga ggtcggccgc gcgcgtgccg agcgcctgcg cggcgctgcc gatggcaccg
     4381 agatggccgc gcaggtcgcc gagcaggcgc gagagtccgg caccgacccg gccgatggcg
     4441 tcttcatcgc tgccgtcgac ctgcacggtc aggtcgccac gcgcggcggc atcgacgcgg
     4501 gcgagcagcg tctcgacctg cgtggccagc tgctcggtgg cggcccgggc ggcggcgagg
     4561 cgttcggcct cggcggcggt ctcgcgttcg cgggcggccg cgcgggtgtc gagcaggtgg
     4621 ttgacgctcg cgaccagcgc gccgtagtcg ccgctgaaac gctcggtgcg cccgcgccgc
     4681 gccagctcgc cgacctcggc cgcgtgcacc agggactgtg tttcggccag cagctcggca
     4741 accgtcgcct gcgtgcgcgc gacgctggtg ccgagccggt cggcctcgct gcgcgccgtg
     4801 tggcggcgcg cggtgtcgcc gcgatcgagc gcatcgaccg cctgcgtcag ctcgcgcagg
     4861 taggcgagca tggcgcggaa ggcatcggcg agcctgccga cctcgtcgcg gccgacatag
     4921 cggacctcgc agctggtgtc gccggcggcg atcgcctgcg cggcttcggc catcgcgccg
     4981 agcggccggg cgatgccgcg cgccagccac caggcgagca ggcagccgag cagcagcagg
     5041 ccggcaccga gcgcgagcgc ggtctggcgg atctgccggg cggtctgctc gaacagggtc
     5101 tggtcctgga acacttccag cgcaccggcc gtgcggccgg cgtaatcggc gatcggcatg
     5161 atcagcaccg ccagatgcgc gtcgccggtg atgatgtcgc ccatcgccgg cgtgcccttg
     5221 gcggcggcgc gcagttcggc ctcgccgagg tcgggccaat tgccggcggt gtcggccagg
     5281 cgccggagct ggccggcgtc atcgagcgtc accagccgcg cgcgcagcag ataccagcgc
     5341 atcagctcgt cgaagaagcc ctgacccatc gccacgccga actcgaccgt gccgagcagc
     5401 tgctcgccgc ggcggatcgg cacgaccgcg cgcaggccga agccgccggc gtcggcttcg
     5461 agcccggcga ccgccttgcc gctctgctgg gcgtcgacga tggtcctgcg cgtggcggag
     5521 agatcgtcgc cgtgctgctg cggctggtgc acgcgcagga aggagatcgc gccgggcccg
     5581 tggaactgga actgttcgag gttctgcccg gttttgagcg ccgcgaacgc gccgccgaac
     5641 tcggcgagca ggcgctcgcg atccccggcc gcgaaggcct cgaccaccga gggctggctc
     5701 gccaccagcg tcgccagcgc ctcggcggcg cgctgctcgc gctgcatcga ggcgatgacc
     5761 agaccgatcg agccgttcag ccgctcggcg cggctcgccg tctggatgcc ggcgaggtcg
     5821 tgcagcatcg cagcggtcag cgtcgcggcg acgatcacga ggatcgcgcc gaatccgagg
     5881 gcgaggcggg tgccgatgcg caggcggcgt agcgtcatgg cgaattcctg aacaggcgat
     5941 gggggggccc gcagcgtgcg gtaccccggg gagccgtttc cctgcgctgg agcaggcgca
     6001 gattttcggc acacgaaaat tcacgatcta tggttaccgg gcggtcggaa cccggttttc
     6061 gccatttcac atcgtgaaaa ctctgcgaaa tggcggcact tcacatcgtg gaaaaataca
     6121 tagatggtct tttaggatgc gcatcggcgg gcgcgagtga tccacgcgca gccagcgtcg
     6181 ctgcagaccg acaacggtag gcgacgcagc agcatctgca gacacacaaa ggatgccgtc
     6241 caggcccgag gtctggttcc cgggaggagg aggacggccg tcgcaacatt cacctcgatg
     6301 ccatgaggtt tcgtcatgac tgccgaacaa tacaaagatg tcgatccgca ggagacccgc
     6361 gaatggctgg ctgccctcga cggtgtcatc gcccatgtgg gcgccgagcg ggcgcactgg
     6421 ctgatcgagg agctgatcga gcgcacgcgg ctggccggtg atctggcccc gttcagcgcc
     6481 aacaccccgt acgtcaacac cgtcccggtc gagaagcagg cgccgatccc gggcgatcag
     6541 gacatcgagc accgcatccg ttcgttcgtg cgctggaacg cggcgatgat ggtgctgcgc
     6601 gccaaccgcg agaccaacgt cggcggccac atcgccagct tcgcgtcggc cgcgaccctc
     6661 tacgaggtcg gctacaacca cttctggcgt gcgccgagcg acggccacaa cggtgacctg
     6721 ctgttcatcc agggtcactc gtcccccggc atctactcgc gcgccttcct gctcgggcgc
     6781 ctgagcgccg agcagatgga caactaccgc caggagaccg acggcaaggg cttgagctcc
     6841 tacccgcatc cgtggctgat gccggacttc tggcagttcc cgaccgtgtc gatgggcctc
     6901 ggcccgctgc tcgcgatcta ccaggcgcgc ttcatgcgct acctcgaaga ccgcggccac
     6961 ggcgtgcaca acaaccgcaa ggtctgggcc ttcctcggcg acggcgagac cgacgagccc
     7021 gagtcgctcg gtgcgatcgg catggccggg cgcgagaagc tcgacaacct gatcttcgtg
     7081 atcaactgca acctgcagcg tctcgacggt ccggtgcgcg gcaacggcaa gatcatccag
     7141 gagctcgaag ccgagttccg cggctccggc tggaacgtca tcaagatcgt ctggggcagc
     7201 aagtgggacg cgctgctcgc ccgcgacaag tccggcatcc tgatgaagcg gatgatggaa
     7261 atcgtcgacg gcgagtacca gacgatgaag gccaaggacg gtgcctacgt ccgcgagagc
     7321 ttcttcaaca cgccggagct gaaggcgctg gttgccgact ggtccgacga cgacatctgg
     7381 cagctcaacc gcggcggtca cgatccgcac aaggtctacg ccggcttcaa ggccgccgtc
     7441 gatcacaagg gtcagccgac cgtgatcctg gccaagacca tcaagggtta cggcatgggt
     7501 gaggccggtg aggcggcgaa catcgcccac caggccaaga agatgaagtt cgatgatctc
     7561 aagggcttcc gcgaccgctt caagattccg gtcagcgacg agcagatccc cgagtatccg
     7621 tacctgacct tcgccgaagg ctcgaaggag ctcgactaca tgcgcgcgcg gcgcatggaa
     7681 ctcggcggct accgcccggg ccgtcacccg agcgcgctgc agctgccggt gcccggcctc
     7741 gcagccttcg atgcgctgct caaggcgacc ggcgagggcc gcgagatcag cacgacgatg
     7801 gccttcgtgc gcatcctcaa cacgctggtc aaggaccgca acgtcggcaa gtacgtcgtg
     7861 ccgatcgtcc cggacgagtc gcgcaccttc ggcatggaag gcatgttccg ccagatcggc
     7921 atctggtcgc aggtcggcca gcagtacacg ccgcaggatg ccgaccagct gatgttctac
     7981 aaggagtcca aggacggcca gatcctgcag gaaggcatca acgaagccgg cgcgatggcc
     8041 gactggatct cggcggcgac ctcctacgcc gtgcacggcg tgccgaccat cccggtctac
     8101 atcttctatt cgatgttcgg cttccagcgc gtcggcgacc tcgcctggct cgccggtgac
     8161 atgcgcgccc gcggcttcct ggtcggcggc accgccggtc gcacgacgct caacggcgag
     8221 ggcctgcagc acgaggacgg ccacagccac ctgtggtcgg cggcggtccc gaactgcatc
     8281 agctacgacc cgactttcgc ctacgagctg gcggtcatcg tgcagtccgg cctcaagcgc
     8341 atgtaccagg accacgagga catctactac tacctgacgg tgatgaacga gaactacgag
     8401 cacccggaga tgccggccgg ggccgaggcc gacatcatca agggcatgta cgccttccgg
     8461 aagggcggcg agtccaaggg gccgcgcgtg cagctgctcg gctcgggcac gatcttccgc
     8521 gaggtcatcg ccgcggccga gctgctgcgc gacgagtggg gtgtcgagtc cgacatctgg
     8581 ggctgcccga gcttcaccga gctgtcgcgc aacggcaacg acaccgtgcg ctggaacctg
     8641 ctgcacccga ccgaggcgcc gcagaagtcg cacgtcgagc agtgcctgga aggcaccgaa
     8701 ggcccggtca tcgccgcgac cgactacatg cgcctgttct cggagcagat ccgcgccttc
     8761 gtgccgcgcc gctacgtgac gctcggcacc gacggcttcg gccgctccga cacccgcgag
     8821 aagctgcgca acttcttcga ggtcgaccgc cgctgggtca cggtcgccgc gctgaaggcg
     8881 ctggccgacg agggcacgat cgagcgcagc aaggtcgccg aggcgatcgc gaagtacggc
     8941 atcgatccgt ccaagccgaa cccgcgcctg atctgaggca ggtccacggc cgcgcgcacc
     9001 ggcagcaccg gtgcgcgccg gatcgacagg attccgtacg gatccttcac gcgtgagtcc
     9061 cattcggcgg tcacggacgc tgacggcgcg ggcagggcgg ttgagcaccg cccgccgcgc
     9121 gccgcggacg gtccgggccg cgaggcaacg agcatgagca atctcatcga agtgaaagtg
     9181 ccggacatcg gcgatttcaa ggacgtggac gtcatcgagg tctccgtcca gcccggcgac
     9241 acggtcaagg tcgaccagac cctgatcgtg ctcgaatccg acaaggcgag catggacgt
//
LOCUS       DAOQIE010000143         9269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3774_length_9269_cov_7.733992, whole genome shotgun sequence.
ACCESSION   DAOQIE010000143 DAOQIE010000000
VERSION     DAOQIE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3774_length_9269_cov_7.733992"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            817..1599
                     /locus_tag="PK144_10085"
     CDS             817..1599
                     /locus_tag="PK144_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF66118.1"
                     /translation="MLRINRLAAALLAAGLLAGASGASATQIININSGSQWLSTTDAL
                     VATQWFHGNFTVGADGVPATAPYPNLVTTPNDIVPGSSAQLMWACGPGSTLCDNTGTN
                     GPTEVWFGMPLDLKANPIARLGAAQIIADDYFEFIVNGQFVQTGYLNDHQNPVSGQPV
                     PIGIDFTSYLHDGYNWLMIHALDGYFPTLGAACDPGYTDRPVQDARGTFCAGDRVGEY
                     AFVDGVIVSVPVPMTAALMGAGLLGLVRRRNRPATGAMRLMA"
     gene            1609..2112
                     /locus_tag="PK144_10090"
     CDS             1609..2112
                     /locus_tag="PK144_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66119.1"
                     /translation="MTITPKPWFTYYDLGVDLQRCFEDNCTRLYQTARQSLSDEEGRE
                     PPARWWQAGLGCLGALRPWLLGWQSLADEQGEYLATALLRCQRQLGEQRERLAEEMLS
                     VWLAGLQRLEPPGPFALLDQLLGLPAASAKQQLPVVAKPGENPPPSESGAQASPAGKS
                     RASAVTS"
     gene            2205..3149
                     /locus_tag="PK144_10095"
     CDS             2205..3149
                     /locus_tag="PK144_10095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF66120.1"
                     /translation="MCPEAAPEAAPSDPDGREAWELGWRRLLDSFGREPAEVAFVFER
                     DGRCRLATTGRAFSCLGQDDGRDWRVRLPEPVRAVIEQLAHLARLGVSTSRGVCCNGR
                     QYQFRSLALRPSEPAPGTVLVLGQDVTEANAAMQALHVLGSRLLGLAERDVLLDIANR
                     RCFERSLQREWRRLRREGGHLAVLFVDLDDFKACNDRFGHAWGDVCLLRVATVLQQVA
                     TRPADLVARYGGDEFAVLLPDTDEAGARLLAGQMHALLQGQQDPQHPRLTLTIGAAAA
                     EAGQLAMASELLDLADRALYRGKTRGRNCTVAASELAG"
     gene            3272..3721
                     /locus_tag="PK144_10100"
     CDS             3272..3721
                     /locus_tag="PK144_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNF66121.1"
                     /translation="MNLVKWDPLRDIEDVTTRVIDLLANPLRRRDGGRESLTVADWAP
                     AVDISETAEAFCLKVELPDVSKEDVHVTLDGDLLTIAGERRQTQTGKDTLVHRVECSY
                     GAFWRSFHLPSPIDAGAIDAQFKDGMLTVKLPKTEVKAVKGIEISIH"
     gene            3947..6643
                     /locus_tag="PK144_10105"
     CDS             3947..6643
                     /locus_tag="PK144_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNF66122.1"
                     /translation="MFGLGKTRPGGGGSAAMAGEVARWDADGALWHLGSRRTGLSETE
                     AATRQRMYGDNRITQAPRTPLWRRLLQEFGNFFARVLWAAAALAFLAESQTPGQGMRE
                     LGFAIVAVILINGVFSFWQVQRAAQALAALQALLPQQVTLLRDGHCERRDSVLLVPGD
                     VIRLGAGERVPADCRLIEACDLRVSLATLSGESAPLTRASAACAQAHPLEADNLLLAG
                     TLLLSGHGTALVYATGMDTEFGRIAGLTQAAEDSASPLQREIAHISRLVTLLATALAV
                     GFFAIGQWLELPLHTSLMFAIGILVANVPEGLLPTVTLALAMATQRMARRRALVRSLP
                     AVEALGSTTVICSDKTGTLTENRMRVCRAATVAGVLAEGEALPGPLAEIARCCHDLQR
                     QADGRMIGDPLEQALHAWAGAGPGCRRIDERAFDAVRRRMSVLVARAGGERWLLCKGA
                     PETVLPRCRQAWTPAGIVTLSHSERSALVRLQSAWADAGQRVLVLAWRPHDDTHIGEE
                     DGLILAGLVGLEDPPRPQVPEAIARCRAAGVRVIMVTGDHPQTALAIAREIGLVCSER
                     PVVLQGDGLVRMSAAQRARALAAPEVICARVSAEQKLLVVEALQAGGEVVAVTGDGVN
                     DAPALKRADVGIAMGLAGTDVAREAADIVLLDDHFATIVDAIEEGRTVYANLRRFLTY
                     ILSSNIPELVPYLVFVLAGVPLPLTVIQILAIDLGTDLLPALALGAEPPRPGVMNRPP
                     RPRGERLLNGALLARAYGWIGLLQAAVSLAVYAAVLDAGGWHYGEALAAQAPLYREAT
                     GACLAAIVVCQMANLFLCRDPRLPAWSAAGHNPLLLPALAAEFGTILVILYTPFGQQL
                     FGTAALPVTVWLQALAGAALLAGLEEGRKALLRAADRWRAPA"
     gene            complement(6785..7051)
                     /locus_tag="PK144_10110"
     CDS             complement(6785..7051)
                     /locus_tag="PK144_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66123.1"
                     /translation="MNARTLITAAILTFAVAGFAQARESDSYPPIEWTHGDAAQTTPA
                     TAPRTAATDSFDARMRATGSDEHYCALDYPLNPKTGTNAEASLC"
     gene            7224..8141
                     /locus_tag="PK144_10115"
     CDS             7224..8141
                     /locus_tag="PK144_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF66124.1"
                     /translation="MDRLVAMAMFVRVVESGSFSAVARELDTTQPNVSKRVRALEARL
                     GSRLIARSTRQLSLTDEGRRYYEDCRRILDAVDAAEHSFLTGREVVAGPLRVAAPVSF
                     GRRRIAARLGDFLDRFPQVTVELLLSDDNSDLVADNIDVALRLGPLADSGLIARSLGQ
                     ARRITAATPGYLARRGEPRTPAELAGHDCLVFTPTPTVNLWHYRHGGERLSVSVGGRV
                     RTNSSEAIRELALAGLGIARAPRWLFGDELADGRIVPILRDYEPEPIPIHAVSPANRR
                     QSARVHAFIDYLASIWAADPQAGITPAGG"
     gene            complement(8122..9111)
                     /locus_tag="PK144_10120"
     CDS             complement(8122..9111)
                     /locus_tag="PK144_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNF66125.1"
                     /translation="MTTFPASVILPAACLCAGLMGYAIQRGATCTVAAVDELLHEHRG
                     TRLLAIVEAALWVLGGLLLARAAGLPVPMPAGYAAGMTTVLGAGLLGAGAALNRACVF
                     GAIARFGNGDWAYLATPFGYWLGCRAVVLLQAMAPQALAQPAPVLQAPALLTLLAGLW
                     LGGRLLAGVLRGLRGGRAGFIRRLAAGVWAPHAATLVIGVTFLLLLALVGPWTYTDLL
                     AELAHGPAMAAGPRLLLFAALLAGAAWGGLSAGRFRPLAPHLSALVRCTAGGALMGLG
                     SLLIPGGNDGLLLLGMPLLWPHAWLAFAVMVVTIALWLTLAARVPLRFSRRPA"
BASE COUNT         1358 a         3193 c         3383 g         1335 t
ORIGIN      
        1 gtggcggctg atccgcagcg ccgggcgcgc gctgccgcag gaggcgttca tcgagccgga
       61 gcacaccgcg caagccggca ccgcgcaagc ctctctcccc cggcttccgg cttcttccga
      121 cggctgagcc gacccccaag gggccgatcc ccgccagcca cgaggccggc ccgcggaccg
      181 gcaaaccctc ccgatgccgg ctttcctcct gccgcacagg cccggaccgc gctgtgcgcc
      241 gattcccggc agtcccctca tgcaggcact gcgtatcgcg tcgatgactg ccggcagagg
      301 cgtgtccggg tgatcggaag agggttcatc catgcgcaaa tcgcatggct ttcgatggcg
      361 attccgggtt gcacctgcga gcaggaatcc atcttctctt gcctcacgct tgcggatgaa
      421 acgcgcctgg cacggaggtt cgcctcactt cagtcggtcc gggcgtcaga tagacccgca
      481 gcgcctgcct gcttgttttc ttgaaaacag aaaaagtcaa gccgtattca tggtgatgaa
      541 atatgacgct caaattttca agttaataaa aatcaagaaa attatgggga cgacacgatg
      601 catgcatggc atttcaatat gcagttcaga aatgcagaaa tgtatccaca ctattttttt
      661 gtgtttaatg caatatacgt ctcacaggta tagattggtc ttaccggcca caggggtgca
      721 gggaaacaat gatgggccga ccgcctgttc acggagggca caagcaagct gccgatatcg
      781 aatcggcatg ggtcaatccg gattcagggg gttccgatgc tcaggatcaa ccgtcttgcc
      841 gcggcactgc tcgccgccgg cctcctcgct ggcgcctctg gtgcctcggc cacgcagatc
      901 atcaacatca acagcggcag ccagtggttg tcgaccaccg acgcgctggt tgccacccaa
      961 tggttccacg gcaacttcac ggtgggagca gatggtgtgc cggcgacggc gccgtatccg
     1021 aatctggtca ccacgccgaa cgacatcgtg cccggtagct cggcgcagct gatgtgggcc
     1081 tgcgggccgg gtagcacgct ctgtgacaac accggcacca acgggccgac cgaggtctgg
     1141 ttcggcatgc cgctcgacct gaaggcgaat ccaatcgccc ggctcggggc ggcgcagatc
     1201 attgccgacg actacttcga attcatcgtc aacggccagt tcgtgcagac cggctatctg
     1261 aatgatcacc agaatccggt cagcggccag ccggtgccga tcggcatcga tttcaccagt
     1321 tacctgcacg atggctacaa ctggctgatg atccatgccc tcgatggcta cttcccgacc
     1381 ctgggtgcgg cctgtgatcc cggctacacc gaccgcccgg ttcaggatgc ccgcggcacg
     1441 ttctgcgccg gggatcgtgt cggcgagtac gccttcgtcg atggcgtcat cgtttcggtg
     1501 ccggtgccga tgaccgcggc gctgatgggc gccgggctgc tcggtctggt acgtcggcgc
     1561 aaccggccgg cgacgggcgc gatgcgcctg atggcctgag gaggtcccat gactattacc
     1621 ccgaaaccct ggttcacgta ctacgacctg ggcgtcgacc tgcagcgctg cttcgaagac
     1681 aactgcacgc gcctttatca gaccgcccgc cagagcctga gcgacgagga ggggcgcgag
     1741 ccgccggccc ggtggtggca ggcggggctc ggctgcctcg gggcgctgcg gccgtggctg
     1801 ctcggctggc agtcgctggc cgatgagcag ggcgaatatc tggcgacggc gctgctgcgc
     1861 tgccagcgcc agctcggcga gcagcgcgag cggcttgccg aagagatgct cagcgtctgg
     1921 ctggctggtc tgcagcgact ggaaccgccg gggccgttcg cactgctcga tcagctgctg
     1981 ggtttgccgg cggcgagtgc gaagcagcag ttgcccgtgg tggcgaagcc cggggaaaac
     2041 ccgccgccgt ccgaatccgg agcgcaggcc agccctgccg gaaagagccg cgccagcgcg
     2101 gtcaccagct gacatggccc gcccctggcc ggggcgctgg cagcgcaacc tgtgtgcctg
     2161 gtaccgggag cggcaccggt tgtaccgggg tggcggccgg gaaggtgtgc cctgaagcgg
     2221 cccccgaggc ggcaccgtcc gatcccgacg gccgcgaagc ctgggagctt ggctggcggc
     2281 gactgctcga cagcttcggg cgcgagcccg cggaggtggc gttcgtgttc gagcgcgacg
     2341 gccgctgccg gctggcgacc accggcaggg cgttttcctg tctgggccag gatgatggcc
     2401 gcgactggcg ggtgcggttg ccggagccgg tgcgcgcagt catcgaacag ctcgcgcatc
     2461 tggcccggct cggggtctcg acaagccgcg gcgtgtgctg caacggacgg cagtaccagt
     2521 tccgcagcct ggcgctgcgc cccagcgaac cggcgcccgg aacggtgctg gtgctggggc
     2581 aggacgtgac cgaagccaac gccgcgatgc aggccctgca cgtgctcggc agccggctgc
     2641 tcgggctggc cgagcgcgac gtgctgctcg acatcgccaa ccggcgctgc ttcgagcgca
     2701 gcctgcagcg ggaatggcgc cggctccggc gcgagggcgg ccacctggcc gtgctgttcg
     2761 tcgatctcga cgacttcaag gcctgcaacg accgcttcgg ccatgcctgg ggtgatgtct
     2821 gcctgctgcg cgtggccacc gtgctgcagc aggtcgcgac ccgcccggcc gatctggtgg
     2881 cccgctacgg cggcgacgag tttgccgtgc tgctgcccga taccgacgag gccggcgccc
     2941 ggctgctcgc cgggcagatg cacgcgctgc tgcagggcca gcaggacccg cagcaccccc
     3001 ggctgacgct gaccatcggc gcggccgcgg cggaggcggg gcagctggcg atggcgagcg
     3061 aactgctcga tctcgccgat cgggcgctgt atcgcggcaa gacccggggg cgcaactgca
     3121 cggttgcggc ttcggagctt gcgggctgaa ccgatgcaag cggcgacgga gtgcctgccg
     3181 tcgccggtgc gtgccggcag tcccgagtcc ggcggaccgg gggactgccg gcagacctgc
     3241 ggtccgcatg aggtttccga gggggtatcc gatgaacctg gtcaagtggg atccgctgcg
     3301 cgacatcgag gacgtcacca cccgggtgat cgatctgctg gccaatccgc tgcgccgacg
     3361 cgacggcgga cgggaaagcc tgaccgtcgc cgactgggcg ccggccgtcg acatcagcga
     3421 gacggccgaa gccttctgcc tgaaggtcga gttgccggac gtcagcaagg aagacgtgca
     3481 tgtgacgctc gacggcgacc tgctgacgat cgccggcgag cggcgccaga cgcagaccgg
     3541 caaggacacg ctcgtgcatc gcgtggagtg cagctacggc gcgttctggc gcagcttcca
     3601 cctgccgagt ccgatcgatg ccggcgcgat cgatgcgcag ttcaaggacg gcatgctgac
     3661 ggtgaagctg ccgaagacgg aggtgaaggc ggtcaagggc atcgagatca gcatccattg
     3721 actggatgac aacggtgcac gcccacccgg cctcggggcg gagcgctctt cggggggagg
     3781 ggctcccgag tgccgggcgg cagcgtgtcg ccagtgagtc cggtcgccat gcgggcggcg
     3841 accggtatcc gggggacgaa aaggtccctg attcggccgg tgcccggaga cgcggcggca
     3901 ccggcctttc ttttttgaag cctgcatgcg ggcgggggca tgagacatgt tcgggctggg
     3961 aaagacgcgc ccgggaggtg gcggcagcgc ggcaatggcc ggcgaggtcg cacgctggga
     4021 cgcggacggg gcgctctggc atctgggcag ccggcgcacg gggctttccg agacggaagc
     4081 cgcgacgcgg cagcggatgt acggcgacaa ccgcatcacg caggctccgc gcacgccgct
     4141 ctggcggcgg ctgctgcagg agttcggcaa tttcttcgcg cgggtgctgt gggcggcggc
     4201 ggcgctggcg tttctggcgg agagccagac gcccggccag ggcatgcgcg aactcggctt
     4261 cgccatcgtc gccgtcatcc tgatcaacgg cgtgttctcg ttctggcagg tgcagcgcgc
     4321 cgcgcaggcg ctggctgcgc tgcaggcgct gctgccgcag caggtgacgc tgctgcgcga
     4381 cgggcactgc gagcggcgcg acagcgtgct gctggtgccg ggggacgtga tccgcctcgg
     4441 ggccggcgag cgggtgccgg ccgactgccg gctgatcgag gcctgcgacc tgcgcgtgag
     4501 cctcgcgacg ctgagcggcg aatcggcacc gctgacccgc gccagcgcgg cctgcgcgca
     4561 ggcgcatccg ctggaggccg acaacctgct gctcgccggc acgctgctgc tcagcggcca
     4621 cggcacggcc ctggtgtacg cgaccggcat ggacaccgag ttcgggcgca tcgccggcct
     4681 gacgcaggcg gccgaagaca gcgcctcgcc gctgcagcgc gagatcgcgc acatctcgcg
     4741 gctggtcacg ctgctcgcca ccgcgctcgc ggtcggcttc ttcgccatcg gccagtggct
     4801 ggagctgccg ctgcacacca gcctgatgtt tgccatcggc atcctggtcg ccaacgtccc
     4861 cgagggtctg ctgccgacgg tgacgctggc actcgccatg gccacgcagc gcatggcgcg
     4921 acgccgggcg ctggtgcgca gcctgccggc ggtcgaggcg ctcggctcga caacggtgat
     4981 ctgttcggac aagaccggca cgctgaccga aaaccgcatg cgcgtgtgcc gcgcggccac
     5041 cgtcgccggc gtgctggccg agggcgaagc gctgccgggg ccgctggccg agatcgcgcg
     5101 ctgctgccac gacctgcagc ggcaggccga cggccgcatg atcggcgatc cgctggaaca
     5161 ggcgctgcac gcctgggccg gggccggacc gggctgccgg cgcatcgacg agcgggcctt
     5221 cgatgccgtg cgccggcgca tgtcggtgct ggtcgcgcgc gccggcggcg agcgctggct
     5281 gctctgcaag ggcgcgcccg aaaccgtgct gccgcgctgc cggcaggcct ggacgccggc
     5341 gggcatcgtc acgctcagcc acagcgaacg ctcggcgctg gtgcgcctgc agtcggcctg
     5401 ggccgatgcc ggccagcgcg tgctggtgct ggcctggcgc ccgcacgacg acacgcacat
     5461 cggcgaggag gacggcctga tcctcgccgg cctcgtcggc ctggaggatc cgccgcgccc
     5521 gcaggtgccc gaggccatcg cccgctgccg cgccgccggc gtacgcgtga tcatggtcac
     5581 cggcgaccat ccgcagaccg cgctcgcgat cgcccgcgag atcggcctgg tgtgcagcga
     5641 gcggccggtc gtgctgcagg gcgacgggct cgtgcgcatg agtgccgcgc agcgtgcccg
     5701 ggcgctcgcc gcgcccgagg tgatctgcgc gcgcgtcagc gccgagcaga agctgctggt
     5761 cgtcgaggcg ctgcaggccg gcggcgaggt cgtcgccgtc accggcgacg gcgtcaacga
     5821 tgcgccggcc ctcaagcgcg ccgatgtcgg catcgccatg ggcctcgccg gcaccgacgt
     5881 ggcgcgcgag gcggccgaca tcgtgctgct cgatgaccac ttcgcgacca tcgtcgatgc
     5941 gatcgaggaa ggccgcacgg tctacgccaa cctccgccgc ttcctgacct acatcctcag
     6001 ctccaacatc ccggagctgg tgccgtatct ggtgttcgtg ctggcgggcg tgccgctgcc
     6061 gctgacggtg atccagatcc tcgcgatcga cctcggcacc gacctgctgc cggccctcgc
     6121 cctcggcgcc gaaccaccgc gcccgggggt gatgaacagg cccccgcgcc cgcgtggcga
     6181 acgcctgctg aacggcgcgc tgctcgcgcg cgcctacggc tggatcggcc tgctgcaggc
     6241 ggccgtgtcg ctcgcggtct acgccgcggt gctcgatgcc ggcggctggc actacggcga
     6301 ggcgctcgcc gcgcaggcgc cgctgtaccg ggaggcgacc ggcgcctgcc tcgcggcgat
     6361 cgtcgtctgc cagatggcca acctgttcct ctgccgcgac ccgcggctgc cggcctggtc
     6421 ggcggccggc cacaacccgc tgctgctgcc ggcactggcg gccgaattcg gcaccatcct
     6481 cgtcatcctc tacacgccct tcggccagca gctgttcggc acggccgcgc tgcccgtgac
     6541 ggtctggctg caggccctgg ccggcgcggc gctgctcgcc gggctggagg aagggcgcaa
     6601 ggcactgctc agggcggccg accgctggcg tgctccggcc tgagggcacc gggccgggcc
     6661 ggaacccgtc gggtcaggac gcgcggacga acgcggcggc ccgaagcctc gccttcacgc
     6721 tttcaaaaag cacagacccc gccgcaacgg ggtctgcagt caggcgcgcg gctgcgccag
     6781 ggcatcagca caggctcgct tcggcgttgg tgccggtctt cgggttcagc gggtaatcga
     6841 gtgcgcagta gtgctcgtcg ctgccggtgg cgcgcatgcg ggcatcgaag ctgtcggtgg
     6901 cggcggtgcg cggcgcggtg gccggggtgg tctgcgcggc gtcgccgtgc gtccattcga
     6961 tcggcgggta gctgtcgctc tcgcgggcct gggcgaaacc ggcgacggcg aaggtcagga
     7021 tggcagcggt gatcagggtt ctggcgttca tggcgtggac ctcgggaggc aggttctggt
     7081 cggatccccc gtcgcggcct tgctgcagtg cggcgggatg gcgtgcagtc tcggccgcgc
     7141 cggccccggc gataagacgc gcaatcgata tagtcggcat gccctggcgg catgaatcgg
     7201 agcatcgggg tatcggagcg aagatggaca gactggtggc gatggcgatg ttcgtgcgcg
     7261 tggtcgaatc gggcagcttc tcggcggtgg cgcgcgaact cgacaccacg cagccgaacg
     7321 tcagcaagcg ggtgcgcgcg cttgaagcgc ggctcggcag ccggctgatc gcgcgcagca
     7381 cgcgccagct gtcgctgacc gacgagggcc ggcgctatta cgaggactgc cggcgcatcc
     7441 tcgatgccgt cgatgccgcc gagcacagct tcctgaccgg gcgcgaggtc gtcgccgggc
     7501 cgctgcgtgt cgccgcgccg gtcagcttcg gccggcggcg gatcgcggcg cggctcgggg
     7561 atttcctgga ccgctttccg caggtgacgg tcgagctgct gctcagcgac gacaacagcg
     7621 atctggtcgc cgacaacatc gacgtggcgc tgcgcctcgg gccgctcgcc gacagcggcc
     7681 tgatcgcgcg ctcgctcggg caggcgcgcc ggatcacggc cgcgacgccg ggatatctcg
     7741 cgcgtcgcgg cgagccgcgc acgccggccg aactcgccgg gcacgactgc ctggtgttca
     7801 cgccgacgcc gaccgtcaac ctctggcact accgccacgg cggcgagcgg ctgtcggtca
     7861 gcgtcggcgg gcgcgtgcgc accaacagct ccgaggcgat ccgcgagctg gcgctcgccg
     7921 ggctcggcat cgcgcgcgcg ccgcgctggc tgttcggcga cgaactggcc gatggccgca
     7981 tcgtgccgat cctgcgcgac tacgagccgg agccgatccc gatccacgcg gtgtcgccgg
     8041 ccaaccgccg ccagtcggcg cgcgtgcatg ccttcatcga ttatctggcg tcgatctggg
     8101 cggccgatcc gcaggccggc atcacgccgg ccggcggctg aagcgcagcg gcacgcgcgc
     8161 tgcgagcgtg agccacagcg cgatggtcac gaccatgacc gcgaacgcga gccaggcgtg
     8221 cggccacagc agcggcatgc cgagcagcag caggccgtcg ttgccgccgg ggatcagcag
     8281 gctgccgaga cccatcagcg caccgccggc cgtgcagcgg accagcgcgc tcaggtgcgg
     8341 tgccagcggc cggaagcgcc cggcgctcag cccgccccag gccgcgccgg cgagcagcgc
     8401 cgcgaacagc agcaggcgcg ggccggcggc catcgccgga ccgtgtgcga gttcggcgag
     8461 cagatcggtg taggtccacg gcccgaccag cgccagcagc agcaggaagg tcacgccgat
     8521 gaccagcgtc gccgcgtgcg gcgcccagac gccggccgcc agccgccgga tgaaacccgc
     8581 gcgcccgccg cgcagtccgc gcagcacgcc ggcgagcagc cgcccgccga gccagaggcc
     8641 ggcaagcagc gtcagcagcg ccggcgcctg cagcaccggg gccggctgcg cgagcgcctg
     8701 cggtgccatg gcctgcagca gcaccacggc gcggcagccg agccagtagc cgaacggcgt
     8761 ggccagatac gcccagtcgc cgttgccgaa gcgcgcgatg gcgccgaaca cgcaggcgcg
     8821 gttcagcgct gcaccggcgc cgagcagccc ggcgccgagc accgtcgtca tgccggcggc
     8881 gtagccggcc ggcatcggta ccggcaggcc ggccgcgcgc gcgagcagca ggccgccgag
     8941 cacccacagc gccgcctcga cgatcgcgag cagccgcgtg ccacggtgtt cgtgcagcag
     9001 ttcgtcgacg gcggcgaccg tgcaggtcgc accgcgctgg atcgcgtagc ccatcaggcc
     9061 cgcgcagaga caggcggcgg ggaggatgac ggaggcggga aaggtggtca tcggcggcgg
     9121 actccgggcg tgggggcgag tgccggtgcg accggctgct gccggcgatc actgccagaa
     9181 tccggccagg tccggatgtg actgaaaaga atcagaagat caacggactg gctgcgctgc
     9241 agtcctttgc ggcgcagacg gagacctcg
//
LOCUS       DAOQIE010000144         9167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3823_length_9167_cov_7.809921, whole genome shotgun sequence.
ACCESSION   DAOQIE010000144 DAOQIE010000000
VERSION     DAOQIE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3823_length_9167_cov_7.809921"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK144_10125"
     CDS             complement(<1..809)
                     /locus_tag="PK144_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNF66126.1"
                     /translation="MADSNTPPFNLPNVNLPDTQKLAENLGKIAERSQSLVQEFAARD
                     NFQVPDPGIVAKSFLELSQKLLADPAKIIEAQMNFWQEYLNVVQATTRRLAGETVEPV
                     VKPAPDDKRFKDEAWVENAVFDFIKQSYLLAARTIHKTATGVEGLDTKTAQRVEFYTR
                     QYIDALAPTNFALTNPRVVKETLESGGENLIKGLANLLEDLDRGKGKLRVRMTDLEAF
                     EPGKNVAATPGKVVFQTPLMQLIQYTPTTEEVYQKPLLIVPPWINKYYILDL"
     gene            1024..1641
                     /locus_tag="PK144_10130"
     CDS             1024..1641
                     /locus_tag="PK144_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66127.1"
                     /translation="MATGGFEGVRRGWLLAVLVAALPLAQADEVEDRVGRGLAAYRKG
                     QYAEALQALAVASSAVQAKLAGEYRNLLPPAPAGWEAEDAEVRAITVPAADGGSGVQL
                     SRRYFREEEELALEVAVDSPLIAGLDLMLSDPKLLASDPAVRPYRIGDNRGLIRRHGD
                     DLEVSVMVGRRVLVRVSGSNLKEDAEAESLLSSIDFAGLSQRLLN"
     gene            complement(1649..3517)
                     /locus_tag="PK144_10135"
     CDS             complement(1649..3517)
                     /locus_tag="PK144_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66128.1"
                     /translation="MRTHPPPRPLHPRLLAELERIGLREARGPVPAALWQRLTEGLSR
                     HFTAWDEEQRHFDHTIEQVYAELARLGASPALRAGAERNTLLTVLNSIADGLCVLDRS
                     GCATLVNPAAVQLLGRPQEALGGRPLLPLLLGPGVEPPDYAGLLARGETWRDEEALFV
                     RGDGERIPVAWSLTPILAGENGEPGGAVLVCRDVARQRELTRSLSHRAAHDPLTGLVN
                     RSEFEQRVAQRIDDGRPHVLMFMDLDRFKQVNDGGGHAAGDALLRGLAIVLAAQIRGS
                     DTLARLGGDEFAVLLDGCPAAHGERVAEKMLAAVRDFRLDWQGQQYAVGVSIGLAALD
                     GRYPGTAEALAGADAACYAAKLAGRDCWRLHAPEATVAAAPVAGSDWGTRLRAAIHDR
                     RLALYAQPIRPLTGGGAPAPADAELFLRLLDPELGELRPGAFLDAAERLQLAAALDRA
                     ALGIVLDTLATRPGDTHRYVLNLGSASLADDEYLPHLRARLAATGVAAGRLAFDVNAR
                     DALAGQPAIGTTMAGLRALGCRVLIDDVGLGLAAPAAWRTLQADGLKLDGALVRALPG
                     GRIEQGFVAAIAALGQALALPVIAESVETAAQLAALQALGIGHGQGEALGAPQRWA"
     gene            complement(3551..4015)
                     /locus_tag="PK144_10140"
     CDS             complement(3551..4015)
                     /locus_tag="PK144_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF66129.1"
                     /translation="MNPPSPPDRSSSDDDHGFPSPEATEFAFYAAIEAADLDGMRRVW
                     EPGEACTCVHPLGPRLQGRPQVLEGWKRIFGSGTRLRFTIGSVRELDGGELRVRLVQE
                     HITVLDADEQPAQPVLATNVYRRGSDGHWRMVLHHASPGRAIAIGGPGGRLH"
     gene            4367..4885
                     /gene="fabA"
                     /locus_tag="PK144_10145"
     CDS             4367..4885
                     /gene="fabA"
                     /locus_tag="PK144_10145"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HNF66130.1"
                     /translation="MSTRQNSYTYEELLACGRGELFGPGNAQLPLPQMLMVDRILSIT
                     EDGGEFGKGQIIAELDVRPDLWFFGCHFTGDPVMPGCLGLDAMWQLVGFYLGWMGGPG
                     RGRALGCGEVKFSGQVLPSAKVITYRISMKRVILRKLVMGIADAVMEVDGRPIYSGKD
                     LRVGLFTSTDGF"
     gene            4896..6116
                     /gene="fabB"
                     /locus_tag="PK144_10150"
     CDS             4896..6116
                     /gene="fabB"
                     /locus_tag="PK144_10150"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HNF66131.1"
                     /translation="MRRVVVTGIGIVSSIGVNRHEVLAALREGRSGIEFSERYRELGL
                     RSQVHGPIRIEKEALIDRKVLRFMGDAAAFNYLAMREAIEDAGLPPGMVSNERTGLIV
                     GSGGGSPEHQVDAVDLLRTKGVRRVGPYMVTRTMSNTTSACLATPFRIRGVNYSISSA
                     CATSAHCIGNAAELIQWGKQDVMFAGGGEEVHANMTVLFDAMGALSSKYNDTPTRASR
                     AYDADRDGFVISGGGGVVVLEALDHALARGAKIYAEITGYGATSDGYDMVAPSGEGAV
                     RCMRQAMATCPTPIDYINAHGTSTPLGDTRELDAVRETFAGGRVPYISSTKSLTGHAL
                     GAAGVNEAIYSLLMLEHDFVAASANIERLDPQAEGLPIALERIDNAGLNTVMSNSFGF
                     GGTNATLIMSRYRG"
     gene            complement(6370..7005)
                     /locus_tag="PK144_10155"
     CDS             complement(6370..7005)
                     /locus_tag="PK144_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNF66132.1"
                     /translation="MGTTLGQRIREAREARGWSQLRLGESVGVSRAAVSQWESGESKG
                     LRPENLVAAARALAVNVEWLVTGVGAMRPDELAAGEGRSDYPLIDWSTAARWPDIGRN
                     AYRVGAEEWLPSPVPLGPAAFVLRVRGCSMAPRLLEGELVYIDPDRPPVHGGIVLVLP
                     GGATEPVLRELIVEGDRRVLRASNPDWPDPLRALSGLDTLIGSVAARLERF"
     gene            7039..7353
                     /locus_tag="PK144_10160"
     CDS             7039..7353
                     /locus_tag="PK144_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66133.1"
                     /translation="MLDPDVKHAYHPSEISGAGPVSGIHNNIICTGRCAIDFPSISVF
                     WFWPYLMLSLGSSVLLVGCLMLRVAWREHMPAHYRLLGGIAVLAGLAILLCAGWLGIR
                     GC"
     gene            complement(7340..7789)
                     /locus_tag="PK144_10165"
     CDS             complement(7340..7789)
                     /locus_tag="PK144_10165"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNF66134.1"
                     /translation="MSPPPALALSGALLQAFLATRYTALTPFGPVEIRIGKRHPVLER
                     ELARCGHDRWVYIGACNPRAQLLDDAHNRRRHLHFGRLLARLGRPGWSGHGLPADAAW
                     PPEPAWLVPGLDAALAHRLALRYGQLAYVCGDRETVAELRCSGLSSP"
     gene            7873..8643
                     /locus_tag="PK144_10170"
     CDS             7873..8643
                     /locus_tag="PK144_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNF66135.1"
                     /translation="MQGEIRISREQAVLCLRVHRPGRRNALTNELYGALADAILGAQD
                     DPAVRVLLITGGEDCFTAGNDLGEFAAGPPADTDAPVFRFLSALVACEKPLVAAVCGV
                     AVGIGTTLLCHCDLVYAAQDARFQLPFVSLGLCPEAASSLLLPRLAGHARASEWLLLG
                     EPFGAAAAQAAGLVNEVLPAGEVLPRALARAQQLARQPPAAVRLTRQLLKAADRAAVE
                     RTLGIEAQAFLGRLASPEAAEAFAAFAEKRAPDFSRFD"
     gene            complement(8667..>9167)
                     /locus_tag="PK144_10175"
     CDS             complement(8667..>9167)
                     /locus_tag="PK144_10175"
                     /inference="COORDINATES: protein motif:HMM:NF017855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNF66136.1"
                     /translation="AVHFATALPWLRWLASELPALLPGLAARLALAGLPNLPPPCPLD
                     VLAGGEWPVPGVDHAYTANTAHILPWRGVIALFAGLARSLRPDGLFFLYGPVLDGPAS
                     ADSNLRFDAALRARDPLQGVRELADLLPLAKAHGFELLSIEAMPRDNVTLIWRLRPAP
                     PAALPT"
BASE COUNT         1306 a         3165 c         3325 g         1371 t
ORIGIN      
        1 aggtcgagga tgtagtactt gttgatccac ggcggcacga tcagcagcgg cttctgatag
       61 acctcttccg tggtcggcgt gtactggatc agctgcatca gcggcgtctg gaacacgacc
      121 ttgcccggcg tcgccgcgac gttcttgccc ggctcgaagg cttcgaggtc ggtcatgcgc
      181 acgcgcagct tgcccttgcc gcgatcgagg tcctccagca ggttcgcgag gcccttgatg
      241 aggttctcgc cgccggattc gagggtttcc ttgaccacgc gcgggttggt cagcgcgaag
      301 ttcgtcggcg ccagggcatc gatgtactgg cgcgtgtaga actcgacgcg ctgcgcggtc
      361 ttggtgtcga ggccctcgac gccggtcgcg gtcttgtgga tcgtgcgcgc ggcgagcagg
      421 tagctctgct tgatgaagtc gaacaccgcg ttctcgaccc aggcctcgtc cttgaagcgc
      481 ttgtcatcgg gcgcgggctt gaccaccggt tcgacggtct cgccggcgag gcgccgcgtc
      541 gtcgcctgca cgacgttcag gtattcctgc cagaagttca tctgcgcttc gatgatcttc
      601 gccggatcgg ccagcagctt ctgcgacagc tccagaaacg acttggcgac gatgcccggg
      661 tccggcacct ggaagttgtc gcgggccgcg aactcctgca cgaggctctg gctgcgctcg
      721 gcgatcttgc cgaggttctc cgcgagcttc tgcgtgtcgg gcaggttgac gttcgggaga
      781 ttgaacggag gcgtgttgct gtcggccatg aaggcgctcc ctggtggtgg attcgccggt
      841 cacggccggc gctgtcatcg tggaaagccg ggcgggcacg ccccggacgg gcagggggtg
      901 ctgcggcggg gccgtacatt aagccttcgc accgcatcgc ggaatacgca gcgggtgcag
      961 gtttgcccga ggccgggcat tgtggaacca tgccgcacga agatttcggc aggtgatccg
     1021 gtgatggcga cgggcgggtt cgagggcgta aggcggggct ggctgctggc cgtgctggtc
     1081 gcggcgctgc cgctggcaca ggcggacgaa gtcgaggatc gcgtcggccg cgggctggcg
     1141 gcgtatcgca agggtcagta cgccgaggcg ctgcaggcgc tggccgtcgc gagcagcgcc
     1201 gtgcaggcga agctcgccgg cgaataccgc aacctgctgc cgccggcgcc ggccggctgg
     1261 gaagccgagg atgccgaggt ccgcgccatc accgtgcctg ctgccgacgg tggcagcggc
     1321 gtgcagctga gccggcgcta cttccgcgag gaagaggaac tcgcgctcga ggtcgccgtc
     1381 gattcgccgc tgatcgccgg gctggacctg atgctgtccg atccgaagct gctcgccagc
     1441 gacccggccg tgcgcccgta ccgcatcggc gacaatcgcg gcctgatccg ccgccacggt
     1501 gacgatctcg aagtgtcggt gatggtcggg cggcgcgtgc tggtgcgcgt cagcggcagc
     1561 aacctcaagg aagacgccga agccgagtcg ctgctgtcgt cgatcgactt cgccggcctg
     1621 tcgcagcggc tgctgaactg acagcccttc aggcccagcg ctgcggcgcg ccgagcgcct
     1681 cgccctggcc gtggccgatg ccgagcgcct gcagcgcggc gagctgtgcc gccgtctcca
     1741 ccgactcggc gatgaccggc agcgccagcg cctgtcccag cgccgcgatc gccgcgacga
     1801 aaccctgctc gatgcgcccc ccgggcaggg cgcgcaccag cgcgccgtcg agcttcaggc
     1861 catcggcctg cagcgtccgc cacgccgccg gagccgcgag tccgagcccg acgtcgtcga
     1921 tcagcacgcg gcagccgagc gcccgcagtc cggccatggt cgtcccgatc gccggctggc
     1981 cggcaagcgc atcgcgggca ttcacgtcga acgcgaggcg gccggcggca accccggtgg
     2041 ccgccaggcg cgcgcgcagg tgcggcagat attcatcatc ggcgaggctc gcgctgccga
     2101 ggttcagcac gtagcggtgc gtatcgccgg ggcgggtcgc cagggtgtcg agcacgatgc
     2161 cgagcgcggc ccgatccagc gccgccgcga gctgcaggcg ctcggccgca tcgaggaagg
     2221 ctccggggcg cagctcgccg agttcgggat cgagcaggcg caggaacagc tcggcatcgg
     2281 ccggcgccgg tgcgcccccg ccggtcaagg gccggatcgg ctgcgcgtac agcgccaggc
     2341 gccggtcatg gatcgccgca cgcagccggg ttccccagtc gctgccggcc accggcgctg
     2401 ccgcgaccgt ggcctcgggg gcgtgcagcc gccagcagtc gcgcccggcg agcttggccg
     2461 cgtagcaggc ggcatcggca ccggccaggg cttcggccgt gcccggatag cggccgtcga
     2521 gcgcggcaag cccgatgctg acgccgaccg cgtactgctg gccctgccag tcgagccgga
     2581 agtcgcgcac ggccgcgagc attttttcgg cgacgcgctc gccgtgggcg gccggacagc
     2641 cgtcgagcag cacggcgaat tcgtcgcccc cgagccgggc gagggtgtcg gagccgcgga
     2701 tctgggccgc gagcacgatg gcgagcccgc gcagcagcgc atcgccggcg gcatgaccgc
     2761 caccgtcgtt gacctgcttg aagcggtcga ggtccatgaa catcagcacg tgcgggcggc
     2821 cgtcgtcgat gcgctgcgcg acgcgctgtt cgaactcgct gcggttgacg aggccggtca
     2881 gcggatcgtg cgcggcccgg tgcgacaggc tgcgcgtcag ctcgcgctgg cgcgccacgt
     2941 cgcgacagac cagcaccgcc ccgccgggct cgccattctc gccggcgagg atcggggtca
     3001 gcgaccacgc caccgggatg cgctcgccgt cgccgcgcac gaacagggcc tcctcgtcac
     3061 gccaggtttc gccgcgcgcg agcaggccgg cgtagtcggg cggctcgacc ccggggccga
     3121 gcagcagcgg cagcagcggc cggccgccga gcgcctcctg cggccggccg agcagctgca
     3181 cggcggccgg gttcaccagc gtcgcgcagc cgctgcgatc gagcacgcac aggccgtcgg
     3241 cgatcgagtt cagcaccgtc agcagcgtgt tgcgttcggc gccggcgcgc agcgccggac
     3301 tggcaccgag ccgggcgagc tcggcgtaaa cctgctcgat ggtgtggtcg aaatgccgct
     3361 gttcctcgtc ccaggccgtg aagtgacggc tcaggccctc ggtcagccgc tgccagaggg
     3421 ctgccggcac cggcccgcgc gcctcgcgca ggccgatgcg ctcgagctcg gcgagcagcc
     3481 gcggatgcag cgggcggggc ggcggatggg tgcgcacggg atgaggccgg aaccgggaag
     3541 cgaggcgcgg ctagtgcagc cgcccgcccg gtccgccgat ggcgatcgcg cgcccgggcg
     3601 aggcgtgatg cagcaccatg cgccagtggc cgtcgctgcc gcgccggtag acgttggtgg
     3661 ccagcaccgg ctgcgccggc tgctcgtcgg catcgagcac ggtgatgtgc tcctgcacga
     3721 ggcgcacgcg cagttcaccg ccgtcgagct cgcgcacgct gccgatcgtg aagcgcaggc
     3781 gcgtgccgct gccgaagatg cgcttccagc cttcgagcac ctgcggccgg ccctgcaggc
     3841 gcggcccgag cggatgcacg caggtgcagg cctcgccggg ctcccagacg cggcgcatgc
     3901 cgtcgagatc ggcggcttcg atggcagcgt agaaggcgaa ttcggtggct tccggactcg
     3961 ggaaaccgtg gtcgtcgtcg gagctgctgc ggtcgggagg cgagggtggg ttcatggccg
     4021 caggacgata cggactcgcc acgccggcgc caagcgccgg tgcccggatt ttgcgtcgtg
     4081 ccggtgccgt atcgctgccg cggtgccgcc gcagcggcgg cggcggcacc tcgtctgcct
     4141 gcacggcagc gtcggcggca cgcacctctg gcgccatgat gtgcgtatgt agctgatccg
     4201 gaaggcttcg ccggtgggcg gctgcgccgc ttgcgggcgt tgcgcatcat ccggaaacct
     4261 gtgcaaacgg cggctgcacc agatgaaaag ccggtatact cgcgcgcctt gtcacgcccg
     4321 tggcacacgt gtcccgggcc gatcctgcca gagaacgcca tctcccgtga gcacccgtca
     4381 gaacagttac acctacgaag aactcctcgc ctgtggtcgc ggcgagctgt tcggccccgg
     4441 caacgcccag ctgccgctgc cgcagatgct gatggtcgat cgcatcctca gcatcaccga
     4501 ggacggtggc gaattcggca agggccagat catcgccgag ctcgacgtgc gccccgacct
     4561 ctggttcttc ggctgccact tcaccggcga tccggtcatg ccgggctgcc tggggctcga
     4621 tgccatgtgg cagctggtcg gtttttacct cggctggatg ggcggaccgg gccgcggccg
     4681 ggcgcttggc tgcggcgagg tcaagttctc gggtcaggtg ctgccgagcg cgaaggtgat
     4741 cacctatcgc atcagcatga agcgcgtgat cctgcgcaag ctggtcatgg gcatcgccga
     4801 tgccgtcatg gaggtcgacg gccgcccgat ctactccggc aaggatctgc gggtcgggct
     4861 gttcacttcc accgacggct tctgaggggg ctgccatgcg tcgcgtcgtc gtcaccggca
     4921 tcggtatcgt ttcgagcatc ggcgtcaacc gccacgaggt gctcgccgcg ctgcgcgagg
     4981 gccgaagcgg catcgagttc agcgagcgct accgggagct gggcctgcgc tcgcaggtcc
     5041 acggcccgat ccgcatcgag aaggaggcgc tgatcgaccg caaggtcctg cgcttcatgg
     5101 gcgatgcggc cgcgttcaac tacctggcga tgcgcgaggc gatcgaggat gccggcctgc
     5161 cgcccggaat ggtctcgaac gagcgcaccg gcctgatcgt cggctccggc ggcggctcgc
     5221 cggaacacca ggtcgatgcc gtcgacctgc tgcgcaccaa gggcgtgcgc cgcgtcgggc
     5281 cgtacatggt cacgcgcacg atgagcaaca cgacctcggc gtgcctggca acgccgtttc
     5341 gcatccgcgg cgtcaactat tcgatcagct cggcctgcgc gaccagcgcg cactgcatcg
     5401 gcaatgccgc cgaactgatc cagtggggca agcaggacgt gatgttcgcc ggcggcggcg
     5461 aggaagtgca tgcgaacatg accgtgctgt tcgacgccat gggcgcgctg tcctcgaagt
     5521 acaacgacac gccgacgcgc gcctcgcgcg cctacgatgc cgatcgtgac gggttcgtga
     5581 tctccggcgg cggtggtgtc gtcgtgctcg aagccctcga tcacgcactg gcacgcggcg
     5641 cgaaaatcta cgccgagatc accggctacg gtgcgacttc cgacggctac gacatggtcg
     5701 ccccgtccgg cgagggcgcg gtgcgctgca tgcgtcaggc gatggcaacc tgcccgacgc
     5761 cgatcgacta catcaacgcc cacggcacct cgaccccgct cggtgacaca cgcgagctcg
     5821 atgcggtgcg cgagaccttc gccggcggtc gcgtgccgta catcagctcg acgaagtcgc
     5881 tgaccggaca cgcgctcggg gccgccggcg tcaacgaggc gatctattcg ctgctgatgc
     5941 tggagcacga cttcgtcgcc gcctccgcca acatcgagcg gctggacccg caggccgaag
     6001 gtctgccgat cgcgctcgaa cgcatcgaca acgccgggct gaacacggtg atgtcgaaca
     6061 gcttcgggtt cggcggcacc aacgcgacgc tgatcatgag caggtatcgc ggctgaagcc
     6121 cgtcagctgc cgtccgtgcg agggcggcgt ccgtttaggg acgtcgcctt tttcattcat
     6181 gcaggccgct gccaggggag ggcagggtgg agaagccggt caggtcggcg ggggaacagg
     6241 tcaggcccgg cagggaacgg ccccggatcg ccgggaagcc ggcaccggtt tggcgggggc
     6301 aggccggcgc ggggccgcaa cacccggacg tggccgcgca accgggcagg cagggctcac
     6361 cggcagggat cagaagcgtt cgagccgggc ggccacgctg ccgatcagcg tatccagccc
     6421 cgacagcgca cgcagcggat cgggccagtc ggggttcgag gcgcgcagca cccgccggtc
     6481 gccctcgacg atcagctcgc gcagcaccgg ttcggtggcg ccgcccggca gcaccagcac
     6541 gatgccgcca tgcaccggcg gccggtcagg gtcgatgtag acgagttcgc cttcgagcag
     6601 gcggggcgcc atgctgcagc cgcgcacgcg cagcacgaac gcggcgggtc cgagcggcac
     6661 cggcgacggc agccattcct cggcgccgac ccggtaggcg ttgcggccga tgtcgggcca
     6721 gcgtgctgcg gtgctccagt cgatcagcgg gtaatcgctg cggccttcgc cggcggcgag
     6781 ttcatcgggg cgcatggcgc cgactcccgt gaccagccac tcgacgttga cggcgagcgc
     6841 acgtgcggcc gccacgaggt tttcggggcg caagcccttc gattccccgc tttcccattg
     6901 cgagacggcc gcacggctga cgccgacgga ttcgcccagc ctgagctgtg accatcccct
     6961 ggcctcccgt gcttcccgga tgcgttgtcc aagtgttgtt cccatggtgc ggcatgctac
     7021 tgccgtaccc gggcaggcat gcttgatccg gatgtcaagc atgcttacca tcctagcgaa
     7081 atttccggag ccggaccagt ctccggcatc cacaacaaca tcatctgcac agggaggtgt
     7141 gccatcgact ttccatccat ctccgtcttc tggttctggc cttacctgat gctgtcgctc
     7201 ggcagctcgg tgctgctggt cggctgtctg atgctgcggg tggcctggcg tgaacacatg
     7261 ccggcgcact accgcctgct cggcgggatc gcagtcctgg ccggtctggc gatcctgctt
     7321 tgcgccggct ggctggggat caggggctgc tgagtccgct gcagcgcagt tcggccaccg
     7381 tttcacggtc gccgcagacg tatgcaagct gaccgtagcg gagtgcaagc cggtgtgcga
     7441 gtgccgcatc cagccccggc accagccagg ccggctccgg cggccaggcg gcatcggccg
     7501 gcaggccgtg gccgctccag cccggccgcc cgagccgcgc cagcagtcgt ccgaagtgca
     7561 ggtgccgcct gcggttgtgc gcatcatcga gcagctgcgc ccgcggattg caggcaccga
     7621 tgtataccca gcggtcgtgg ccgcaccgtg ccagctcgcg ctcgaggacc ggatgccgct
     7681 tgccgatgcg gatctcgacc ggaccgaacg gcgtcagcgc cgtgtagcgg gtcgccagaa
     7741 acgcctgcag cagcgccccg gacagcgcaa gggccggggg cggactcatg cccgatcacc
     7801 cggcccggca ctggccgtgg acccgggctc ggcgtagctt cgtgccggta gcggatcact
     7861 gggggcatcg gcatgcaggg tgaaatccgg atcagccgcg aacaggccgt gctctgcctg
     7921 cgcgtgcatc ggcccgggcg gcgcaacgcc ctgaccaacg aactgtatgg cgcgctcgcc
     7981 gatgcgatcc tcggcgcgca ggacgacccg gccgtgcgtg tgctgctgat caccggcggc
     8041 gaggactgct tcacggccgg caatgatctc ggcgaattcg cggccggtcc gccggccgac
     8101 accgatgcac cggtgttccg tttcctgagc gccctggtcg cctgcgagaa gccgctggtt
     8161 gcggccgtct gcggcgtggc ggtcggcatc ggcacgacgc tgctctgcca ctgcgatctc
     8221 gtctacgccg cgcaggatgc ccgcttccag ctgccgttcg tcagcctcgg cctctgcccg
     8281 gaagcggctt ccagcctgct gctgccgcgg cttgccggcc atgcgcgtgc ctccgaatgg
     8341 ctgctgctcg gcgagccgtt cggggccgcg gccgcgcagg cggcggggct ggtcaacgag
     8401 gtgctgcccg ccggcgaagt cctgccccgc gcgctggccc gcgcgcagca gctcgcccgc
     8461 cagccgccgg ccgccgtgcg cctgacccgg cagttgctga aggccgccga ccgggcggcg
     8521 gtcgagcgca cgctgggcat cgaggcgcag gcctttctgg gccggctggc ctccccggag
     8581 gccgcggagg ctttcgcggc gttcgctgag aagcgcgcgc cggacttctc gcgcttcgac
     8641 tgatccgctc gcggcctgca ggcgattcat gtcggcaggg cggcaggcgg cgcgggccgc
     8701 aagcgccaga tcagcgtcac gttgtcgcgc ggcatcgctt cgatcgacag aagctcgaag
     8761 ccgtgcgcct tcgccagggg cagcagatca gcgagttcgc gcacgccctg cagcggatcg
     8821 cgggcgcgca gcgcggcatc gaagcgcagg ttgctgtccg ccgaggccgg gccgtcgagc
     8881 accggcccgt acaggaagaa caggccatcc gggcgcagcg agcgcgccag tccggcgaac
     8941 agggcgatga cgccgcgcca cggcaggatg tgggcggtat tggccgtgta ggcgtgatcg
     9001 acaccgggca ccggccattc gccgccggcc agcacatcca gcgggcaggg cggcggcagg
     9061 ttcggcagcc cggcgagcgc gagccgggcg gcaagaccgg gcagcagggc cggcagctca
     9121 ctcgcgagcc agcgcagcca gggcagggcg gtggcgaaat gcacggc
//
LOCUS       DAOQIE010000145         9148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3837_length_9148_cov_6.232377, whole genome shotgun sequence.
ACCESSION   DAOQIE010000145 DAOQIE010000000
VERSION     DAOQIE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_9148_cov_6.232377"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PK144_10180"
     CDS             <1..634
                     /locus_tag="PK144_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF66137.1"
                     /translation="DADAALAEARRDWQALTGLDALPRRIDERAAQEPQLHPERLAAL
                     AASRAAAGRVELAGRSAEERTKLRFGVRGERAARGEDEDYSLAVAVSIPFGGERFRRA
                     EVESARAELAEAAAEEARLADTLPGALAAARAQLALERERLAALQTRQSLARERSRLA
                     GRAHDLGQLDLPALLQARAAAIEVEAEAELQGLRVARAVARLNQTQGVLP"
     gene            631..2364
                     /locus_tag="PK144_10185"
     CDS             631..2364
                     /locus_tag="PK144_10185"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF66138.1"
                     /translation="MSRQDWRQHLPRTPSLLTSALPALTVLVLVLALAGPAPVSAHGG
                     EDHSHEAAAAAPALPVALAPRFEATSPAFQLLGILENGRLTLYLDRFADNAPVAGATL
                     TVEVDGRTQPAPETGAATYALAETGALANPGTHELLVSVTAGDEQDVLIGTLSVPPAA
                     ASGAAAPAAAGGPGSIGGPGWGLAGLALLLGYGLGRLGRRTAPASPLLIAGALGLALA
                     ALPPAAHAHGGEDHGHDEAAPLPATLTGDAPRRLPDGSVFVPKPVGRLLGLRTQLAAM
                     ATLAQTVVVPGHLIADPNRSGRVQAPQAGRIVPAAGGIAHLGQPVRAGEVLAWLEPLA
                     ASLEAAGQSAGLAELDSQIELATQRLTRLQQLAGSVAQREIDEARTELAGLRKRRSAL
                     GTGLGGREPLRAPVDGVVSRSTLVAGQIVEARETLVEILDPGAWWVEAVVFDAGLAGN
                     IDAALAETDNGRTYRLGFLGMGFERRAQGLPMQWRVDGDGRGLSAGQPVRLLLQTRSR
                     SRGVPLPVAALVRGADGESRVWVQAGIERFVPRRVQVQPLDGERVLVTGGLADGERFL
                     VIGASLLAQLR"
     gene            2381..5506
                     /locus_tag="PK144_10190"
     CDS             2381..5506
                     /locus_tag="PK144_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF66139.1"
                     /translation="MFDRLIRTSLALRLPTLVAALVLLVWGVQLARELPVDVFPDLNR
                     PTVTLMTEAEGMAPEEVESLVTVPLETAMNGLAGVRRVRSVSGVGLSVLYVEFDWGTD
                     VFRNRQQVAERIGSVQAQLPPGIVPQMGPVTSIMGEIMLIALKAGDGSGDGHGGEAVT
                     PMQLRDLADWTLRPQLLAIAGVAQAIPIGGEVRQIQVVPDPALMEDLDVTPQQLVEAL
                     QGFGANVSGGFLDHGGREVLIRALGRSARIADLEGLVVAVRDDRPVYLKQLASVRVAA
                     RTKRGDASVDGRPGVIVSVQKQPSADTVDLSRRIDATLANLARTLPPGVQVEVLFRQA
                     NFISAAVGNVEDALRDGAIMVAIVLFLFLLNVRTTLISLTAIPLSLLVAVLVFEAFGL
                     SINTMTLGGLAIAIGELVDDAVVGVENVLRRLRQNRALAEPRPALAVIAQATLEVRSA
                     IFYATLIIVLVFVPLFALAGIEGRLFTPLGIAYMVSMLASMLVSVTVTPVLCHYLLAR
                     GGVLEHGDSPLVAWLKRQDARLLGWSFRHTQGLALAAVGLVLAAGYGLTQLPRAFLPA
                     FNEGSYTVSLLLNPGTSLAESNRIGTLAERLLLSVPEVVHTGRRTGRAELDEHAEGVH
                     SSEIDVDLKPSERPRAVVLAELRARLALLPAAVNIGQPISHRLDHLLSGVRAQIALKL
                     FGDDPDTLRGLAAGLEEKLRTLPGLVDVQLEKQVPIPQVQVQLDYARAARYGVTPAAL
                     GEALETLVNGRVVTQLIERERRYELVLRLAEAERTPQRLGELLVGTPGGWIPLRLVAD
                     IIETVGPNQIARDDGRRRIVISANGDGRDMAAIVADLRALLAATPLPEGYHTTLEGQF
                     QAQESATRLIAGLALAAVGLIFVVLYARYRAVVFALLIMANIPLATVGGVAALWLAGQ
                     VLSVASLVGFITLAGISTRNGILKVSHYLNLMLHEGEDFSDAMIIRGSLERLTPVLMT
                     ALVAATGLVPLLLTPDAPGREILYPVAVVIFGGLISSTVLDTILTPVLFRRFGRAAAL
                     RLAAAGGEERY"
     gene            5611..5985
                     /locus_tag="PK144_10195"
     CDS             5611..5985
                     /locus_tag="PK144_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66140.1"
                     /translation="MNTPFNTLFPKLFLVALAGLPAAVLAHGDAATGAHGGTLIESGE
                     VVLEWIGARNTICLSDHGSGAPIPSAQAGGRIIVAGAGAQELTPADGNCLRYPTALAA
                     GSKAVVSIRLPGRPPVQARLEP"
     gene            complement(6342..7268)
                     /gene="ntrB"
                     /locus_tag="PK144_10200"
     CDS             complement(6342..7268)
                     /gene="ntrB"
                     /locus_tag="PK144_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNF66141.1"
                     /translation="MSATSANIIPLPVASAEPAAAPAPATAAAEPVAADSAPAYQPVP
                     RWRQHLAAFARTVIPPLLTIALLLLVWQLLCARPGASLPAPSKVLEDTWQLISDPFFD
                     NGGTDKGLFWHMLASLKRVAYGYAIAAVVGVALGALIGCSVWAMRGLDPLFQVLRTIP
                     PLAWLPLSLAAFRDGEPSAIFVIFITAVWPIIINTAVGIRNIPQDYQNVARVLRMNAW
                     EYSTIIMLPAAAPYIFTGLRIGIGLSWLAIVAAEMLIGGTGIGFFIWDAWNSSLISDI
                     ILALVYVGLTGFFLDRLIAFAGRLATRGTASA"
     gene            complement(7346..8635)
                     /locus_tag="PK144_10205"
     CDS             complement(7346..8635)
                     /locus_tag="PK144_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNF66142.1"
                     /translation="MSDQNSKFSRRQFLKAAAVSAAAGLMPGLLPRPLYAAADAPETT
                     KAILGFIALTDAAPLIVAKEKGMFAKYGMPDVEVVKQASWGTTRDNLVLGSQGNGIDG
                     AHILTPMPYLISTGKVTQNNVPTPMYILARLNLAGQCISVGAEYLDLKIGLDAKPFKT
                     ALEAKKASGKAVKAAMTFPGGTHDLWIRYWLAAGGIDPDKDIETIVVPPPQMVANMKV
                     GTMDCFCVCEPWNEQLKNQKIGYTALTTGELWNGHPEKAFAMRADWVDKNPKATQALL
                     LAVMEAQQWCDKPENREEMAKIVSKRQWINAPFEDIVARMKGTFDYGTGKVVENSPFI
                     MKYWADHASYPYQSHDLWFLTENVRWGKFDGSEDFKALIAKVNREDLWRQAATTAGVT
                     SLPASPSRGPEKFFDGKVFDPANPAAYLASLDIKRKG"
BASE COUNT         1225 a         3208 c         3393 g         1322 t
ORIGIN      
        1 cgatgccgat gccgcgctcg ccgaggccag gcgcgactgg caggcgctga ccggcctcga
       61 tgcgctgccg cggcgcatcg acgagcgtgc ggcgcaggag ccgcagctgc atccggagcg
      121 cctcgccgcg ctggccgcga gccgggccgc cgccggtcgc gtcgagctgg ccggacgcag
      181 cgccgaggag cgcacgaagc tcaggttcgg cgtgcgcggc gaacgcgccg cccgcggcga
      241 ggacgaggac tacagcctgg ccgtggccgt cagcatcccg ttcggcggcg agcgtttccg
      301 ccgcgccgag gtcgagagcg cgcgcgccga gcttgccgag gccgcggccg aggaagcgcg
      361 gctcgccgac acgctgcccg gcgcgctcgc cgcggcacgc gcacagctcg cgctcgaacg
      421 cgaacggctg gcggcgctgc agacgcgcca gtcgctcgcg cgcgaacgca gccgtctcgc
      481 cgggcgcgcc cacgacctcg gccagctcga cctgccggcg ctgctgcagg cgcgcgccgc
      541 cgccatcgag gtcgaggccg aagccgaact gcagggcctg cgcgtcgcgc gcgccgtcgc
      601 gcggctgaat cagacgcagg gggtgctgcc atgagccggc aggactggcg gcagcacctg
      661 ccgcgcacac catccctgct gacatccgcg ctgccggcgc tgaccgtgct cgtgctcgtg
      721 ctggccctcg ccggcccggc gccggtgtcc gcgcacggcg gcgaggacca cagccacgag
      781 gccgctgccg ccgccccggc gctgccggtg gcgctggccc cgcgcttcga ggcgacgagt
      841 ccggccttcc agctgctcgg catcctcgag aacggccggc tgacgctgta tctcgaccgc
      901 ttcgccgaca acgcgccggt cgccggcgcg acgctgacgg tcgaggtcga tggccgcacg
      961 cagcccgccc ccgaaaccgg cgcggccacc tacgcgctcg ccgagaccgg cgcgctcgcg
     1021 aaccccggca cgcacgagct gctggtcagc gtcacggccg gcgacgagca ggacgtgctg
     1081 atcggcacgc tgagcgtgcc gccggccgcg gcgtccggag cggcggcccc ggccgctgcg
     1141 ggcggcccgg gcagcatcgg cggccccggc tgggggctgg ctggactggc gctgctgctc
     1201 ggctacggcc tgggccggct cggacgccgc actgcaccgg catcgccgct gctgatcgcc
     1261 ggtgcgctcg ggctcgcgct cgcggcgctg ccgccggcgg cgcacgcgca cggcggcgag
     1321 gatcacggtc acgacgaggc cgcgccgctg ccggcgacgc tgaccggcga tgcgccgcgg
     1381 cggctgcccg atggcagcgt gttcgtgccg aagccggtcg ggcgcctgct cgggctgcgc
     1441 acgcagctcg cggcgatggc gacgctcgcg cagaccgtcg tcgtgccggg gcatctgatc
     1501 gccgatccga accgcagcgg ccgcgtgcag gcaccgcagg ccggacgcat cgtgccggcg
     1561 gccggcggca tcgcgcacct cggccagccg gtgcgcgccg gcgaggtgct ggcctggctg
     1621 gagccgctgg cggcgagcct cgaagccgcc ggccagagcg ccggactcgc cgagctcgac
     1681 agccagatcg aactggccac gcagcgcctg acgcgcctgc agcagctcgc cggcagcgtc
     1741 gcgcagcgcg agatcgacga ggcgcgcacc gagcttgccg gcctgcgcaa gcgccgttcg
     1801 gcgctcggca ccggactcgg cgggcgcgag ccgctgcgcg cgccggtcga tggcgtcgtc
     1861 agccgcagca cgctggtcgc cggccagatc gtcgaggcgc gcgagacgct ggtcgagatc
     1921 ctcgaccccg gcgcctggtg ggtcgaggcc gtggtgttcg atgccggcct cgccggcaac
     1981 atcgacgccg cactcgccga aaccgacaac ggccgcacgt accggctcgg cttcctcggc
     2041 atgggcttcg agcggcgcgc ccagggcctg ccgatgcagt ggcgcgtcga cggggacggc
     2101 cgcggcctca gcgccggcca gccggtgcgg ctgctgctgc agacgcgcag ccgcagccgc
     2161 ggcgtgccgc tgccggtcgc cgccctcgtg cgcggggccg atggcgagtc gcgcgtctgg
     2221 gtgcaggccg gcatcgagcg cttcgtgccg cggcgcgtgc aggtccagcc gctcgacggc
     2281 gagcgcgtgc tggtcaccgg ggggctggcc gacggcgagc gcttcctggt cattggcgcg
     2341 agcctgctcg cgcagctgcg ctgaagcgga gggcgctgtc atgttcgatc gcctgatccg
     2401 caccagcctg gcgctgcgcc tgccgacgct ggtcgccgcg ctggtgctgc tggtctgggg
     2461 cgtgcagctc gcacgcgaac tgccggtcga tgtgttcccg gacctcaacc gcccgaccgt
     2521 gacgctgatg accgaagccg agggcatggc gcccgaggag gtcgagagcc tggtcacggt
     2581 gccgctcgaa accgcgatga acggcctggc cggcgtgcgc cgcgtgcgct cggtgtccgg
     2641 cgtcggcctg tcggtgctct acgtcgagtt cgactggggc accgacgtgt tccgcaaccg
     2701 ccagcaggtc gccgagcgca tcggcagcgt gcaggcgcag ctgccgccgg gcatcgtgcc
     2761 gcagatgggg ccggtcacgt cgatcatggg cgagatcatg ctgatcgcgc tgaaggccgg
     2821 cgacggcagc ggcgacgggc acggcggcga agccgtgacg ccgatgcaac tgcgcgatct
     2881 ggccgactgg acgctgcgcc cgcagctgct cgccatcgcc ggcgttgcgc aggcgatccc
     2941 gatcggcggc gaggtgcgcc agatccaggt cgtgcccgac ccggcgctga tggaggacct
     3001 cgacgtgacg ccgcagcagc tcgtcgaggc gctgcagggc ttcggcgcca acgtcagcgg
     3061 cggcttcctc gatcacggcg ggcgcgaggt gctgatccgc gcgctcgggc gcagcgcccg
     3121 catcgccgat ctcgaagggc tggtggtcgc ggtgcgcgac gaccggccgg tgtacctcaa
     3181 gcagctcgcc agcgtgcgcg tcgccgcgcg caccaagcgc ggcgacgcca gcgtcgacgg
     3241 ccggcccggc gtcatcgtct cggtgcagaa gcagccgagc gccgacaccg tggacctgag
     3301 ccggcgcatc gacgcgacgc tcgcgaatct cgcgcgcacg ctgccgcccg gcgtgcaggt
     3361 cgaggtgctg ttccggcagg cgaacttcat ctcggccgcg gtcggcaacg tcgaggatgc
     3421 gctgcgcgac ggcgcgatca tggtcgcgat cgtgctgttc ctgttcctgc tcaacgtgcg
     3481 cacgacgctg atctcgctga ccgcgatccc gctgtcgctg ctggtggcgg tgctggtgtt
     3541 cgaggcgttc gggctttcga tcaacacgat gacgctcggc ggactcgcga tcgcgatcgg
     3601 cgagctggtc gacgatgccg tggtcggcgt cgagaacgtg ctgcggcggc tgcgccagaa
     3661 ccgggcgctc gccgaaccgc gcccggcgct cgcggtgatc gcgcaggcga cgctcgaggt
     3721 gcgctcggcg atcttctacg cgacgctgat catcgtgctg gtgttcgtgc cgctgttcgc
     3781 gctcgccggc atcgaggggc ggctgttcac gccgctcggc atcgcctaca tggtgtcgat
     3841 gctcgcgagc atgctggtgt cggtgacggt gacgccggtg ctctgccact acctgctcgc
     3901 gcgcgggggc gtgctcgaac acggtgacag cccgctggtg gcctggctca agcgccagga
     3961 tgcgcgcctg ctcggctggt cgttccggca cacgcagggg ctggcgctgg ccgcggtcgg
     4021 actggtgctc gcggccggct acggcctcac gcagctgccg cgtgccttcc tgccggcctt
     4081 caacgagggc agctacacgg tcagcctgct gctgaacccc ggcacctcgc tcgccgagtc
     4141 gaaccgcatc ggcacgctgg ccgagcgcct gctgctgtcg gtgcccgagg tcgtgcacac
     4201 cggccggcgc accggccgcg ccgaactcga cgagcacgcc gagggcgtgc attcgagcga
     4261 gatcgatgtc gacctgaagc cttcggaacg tccgcgcgcc gtcgtgctcg ccgagctgcg
     4321 cgcgcggctc gcactgctgc cggcggcggt caacatcggc cagccgatct cgcaccggct
     4381 cgatcacctg ctctcgggcg tgcgcgcgca gatcgcgctg aagctgttcg gcgatgaccc
     4441 ggacacgctg cgcggcctcg ccgccgggct ggaggaaaaa ctgcgcacgc tgcccggcct
     4501 cgtcgacgtg cagctcgaga agcaggtgcc gatcccgcag gtgcaggtgc agctcgacta
     4561 cgcgcgcgcc gcacgctacg gcgtgacccc ggccgcgctc ggcgaggcgc tggagacgct
     4621 ggtcaacggc cgcgtcgtca cgcagctgat cgagcgcgaa cggcgctacg agctggtgct
     4681 gcgtctggcc gaggccgagc gcacgccgca gcgcctcggc gagctgctgg tcggcacgcc
     4741 gggcggctgg attccgctgc gcctggtcgc cgacatcatc gagaccgtcg gcccgaacca
     4801 gatcgcccgc gacgacggcc gccggcgcat cgtgatctcg gccaacggcg atggccgcga
     4861 catggcggcg atcgtcgccg acctgcgcgc gctgctggcg gcaacgccgc tgcccgaggg
     4921 ctaccacacg acgctcgaag gccagttcca ggcccaggaa tcggcgacgc ggctgatcgc
     4981 cgggctggcg ctcgccgcgg tcgggctgat cttcgtcgtg ctgtacgcgc gctaccgcgc
     5041 ggtggtgttc gcgctgctga tcatggccaa catcccgctc gcgaccgtcg gcggcgtggc
     5101 ggcgctgtgg ctggccgggc aggtgctgtc ggtcgcgagt ctggtcggct tcatcacgct
     5161 cgccggcatc agcacgcgca acggcatcct gaaggtcagc cactacctga acctgatgct
     5221 gcacgagggc gaggacttca gcgacgcgat gatcatccgc ggcagcctcg aacgcctgac
     5281 gccggtgctg atgacggcgc tggtcgcggc caccggcctg gtgccgctgc tgctgacgcc
     5341 ggacgcaccg gggcgcgaga tcctgtaccc ggtcgcggtc gtgatcttcg gcggcctgat
     5401 cagctcgacc gtgctcgaca cgatcctgac gccggtgctg ttccgccgct tcggccgcgc
     5461 ggcggcgctg aggctcgcgg cggccggcgg cgaggagcgc tactgatggg caccggtggc
     5521 cggctgccgg cgatccgcgc ctcgccggcc ggccccggcc ctgcgcgctg cccgcctgca
     5581 tccccccgac tgatccggag ttccgaagcc atgaatacgc cgtttaacac cctgtttccg
     5641 aagctgttcc tcgtcgcgct ggccggcctg ccggcggccg tcctcgccca cggcgacgcc
     5701 gccaccggcg cgcacggcgg cacgctgatc gagtccggcg aggtcgtgct cgaatggatc
     5761 ggcgcgcgca acaccatctg cctcagcgat cacggcagcg gcgccccgat cccgagcgcg
     5821 caggccggtg gccggatcat cgtcgccggc gccggcgcgc aggaactcac gccggccgac
     5881 ggcaactgcc tgcgctatcc gacggcgctc gccgccggca gcaaggccgt cgtctcgatc
     5941 cggctgccgg ggcggccgcc ggtgcaggcg cggctggaac cgtgagcccg cctgccccgg
     6001 cgggcatggg tatctacaca agtcagcggt tccgcggaca ggatggatcg accaccaacg
     6061 accgcgcgtc gatctcccct ctccccttgc gggagagggg ccgggggaga ggggtagacg
     6121 ccggcactgc gtccggacct tgccccctct ccccaacccc tcccccgcga gtggggaggg
     6181 gcttgttcat caatgccttg ccgtcgttct gatccatccg cgctgtccgg acaagtccgg
     6241 gaaccgatgc ggcaagttgt gtggataccg atgcccggcg gaacggggca atgaaaagcc
     6301 ccggccgcgc gcagggagcg cggccggggc cggaggggcg ctcaggccga ggcggtgccg
     6361 cgggtggcga ggcggccggc gaaggcgatc aggcgatcga ggaagaagcc ggtcaggccg
     6421 acgtagacca gcgccaggat gatgtcgctg atcagcgagc tgttccacgc atcccagatg
     6481 aagaagccga tgccggtgcc gccgatcagc atctcggcgg cgacgatcgc gagccaggac
     6541 aggccgatgc cgatgcgcag gccggtgaag atgtacggcg cggccgccgg cagcatgatg
     6601 atcgtcgagt attcccaggc gttcatgcgc agcacgcggg cgacgttctg gtagtcctgc
     6661 ggaatgttcc ggatgccgac cgcggtgttg atgatgatcg gccagaccgc ggtgatgaag
     6721 atcacgaaga tcgccgaggg ctcgccgtcg cgaaacgccg ccagcgacag cggcagccac
     6781 gcgagcggcg gaatcgtgcg cagcacctgg aacagcggat cgagcccgcg catcgcccag
     6841 acgctgcagc cgatcagcgc gccgagggcc acgccgacga cggcggcgat cgcatagccg
     6901 taggcgacgc gcttgaggct ggccagcatg tgccagaaca ggcccttgtc ggtgccgccg
     6961 ttgtcgaaga acggatcgga gatcagctgc caggtgtctt ccagcacctt cgacggggcc
     7021 ggcaggctgg cgccgggccg ggcacacagc agctgccaga ccagcagcag cagcgcgatg
     7081 gtcagcagcg gcggaatgac ggtgcgggcg aacgcggcga ggtgctggcg ccagcgcgga
     7141 accggctggt aggccggtgc gctgtcggcg gcgaccggct cggccgcggc ggtggcggga
     7201 gcaggcgccg cggccggttc cgcactggcg acgggaaggg gaatgatgtt ggcgctggtg
     7261 gcactcatcg cgcggcactc cgggggtgga tgctcaggga agcggcggcc ggcggtgccc
     7321 gcgcacgccg gtccggcggg ctgcgtcagc ccttgcgctt gatgtcgagg ctcgcgaggt
     7381 aggcggccgg attggccgga tcgaacacct tgccgtcgaa gaacttctcg gggccgcgcg
     7441 acggcgaggc cggcaggctg gtgacgccgg cggtcgtggc ggcctggcgc cagaggtcct
     7501 cgcggttgac cttcgcgatc agggccttga agtcctcgct gccgtcgaac ttgccccagc
     7561 gcacgttctc ggtcaggaac cagaggtcgt ggctctggta cgggtaggag gcgtgatcgg
     7621 cccagtactt catgatgaag gggctgttct cgaccacctt gccggtgccg tagtcgaagg
     7681 tgcccttcat gcgcgcgacg atgtcctcga acggcgcgtt gatccactgg cgcttggaga
     7741 cgatcttcgc catctcctcg cggttctcgg gcttgtcgca ccactgctgg gcttccatga
     7801 ccgcgagcag cagcgcctgc gtcgccttcg ggttcttgtc gacccagtcg gcgcgcatcg
     7861 cgaaggcttt ctcggggtgg ccgttccaga gctcgccggt ggtcagggcc gtgtagccga
     7921 tcttctggtt cttcagctgc tcgttccacg gctcgcagac gcagaagcag tccatcgtgc
     7981 cgaccttcat gttcgcgacc atctgcggcg gcggcacgac gatggtctcg atgtccttgt
     8041 ccgggtcgat gccgccggcg gcgagccagt agcggatcca gagatcgtgc gtgccgcccg
     8101 ggaaggtcat cgccgccttg accgccttgc cggaggcctt cttggcttcc agcgccgtct
     8161 tgaacggctt ggcgtcgagg ccgatcttga ggtcgaggta ctcggcaccg accgagatgc
     8221 actgaccggc gaggttcagg cgggcgagga tgtacatcgg cgtcggcacg ttgttctgcg
     8281 tgaccttgcc ggtggagatc agatacggca tcggcgtcag gatgtgcgca ccgtcgatgc
     8341 cgttgccctg cgagccgagc acgaggttgt cgcgcgtcgt gccccaggag gcctgcttga
     8401 cgacctcgac atcgggcatg ccgtacttcg cgaacatgcc cttttccttg gcgacgatca
     8461 gcggcgcggc gtcggtcagc gcgatgaagc cgaggatggc cttggtcgtt tccggcgcgt
     8521 cagcggctgc atacagcggg cgcggcagca ggcccggcat gagcccggcc gccgcggaga
     8581 cagcggcggc cttcaggaac tggcggcgcg agaacttgga attctgatcg gacatgaggg
     8641 cgtgcgctcc tggtgcgatg aggatcgccc cgcaggcccg cacgccgcgg cgacgatccg
     8701 tgatgacggt ggggccggcc gtcaggcatc gccaacggac cgcaaaccac cgtgcttacg
     8761 taatccaccc gccgttgggt gaaggaatca ggccggagac cgccgttggt ccccgttcgc
     8821 gatcattaaa caaagttcat gccagcccct gaatcgtgca tggtttttgc cgtgcgccgc
     8881 tgacttgcct gcaggctgcg ctacgaaatt cgtgattcag gtcagtggct tgccgtgccg
     8941 gtgcgggtgc agggcgcaag cccggggcgg ggcatgggcc gggcggcatg gcggttcgta
     9001 tcagtgcggt gcataccccc ttggtggcat gcatcatgct ggtgcgctac ctcgcccgga
     9061 agcgtgcagc cccgtgcgca ggcattgccg gggccggcag cccggttgcc gggatggcgg
     9121 gggaggggcg tctgcgggcc gggtggcg
//
LOCUS       DAOQIE010000146         9126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3850_length_9126_cov_7.585162, whole genome shotgun sequence.
ACCESSION   DAOQIE010000146 DAOQIE010000000
VERSION     DAOQIE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3850_length_9126_cov_7.585162"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2050
                     /gene="recQ"
                     /locus_tag="PK144_10210"
     CDS             <1..2050
                     /gene="recQ"
                     /locus_tag="PK144_10210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNF66143.1"
                     /translation="GGGKSLCYQIPALLRAGLGLVVSPLIALMHDQVDALTQLGVRAA
                     YLNSSLDAASVRETERAFAAGELDLLYVAPERLVTERCLGLLARTPLALFAIDEAHCV
                     SQWGHDFRPEYRALSVLHERFPDVPRVALTATADALTRDDIREHLGLLDARVFVSSFD
                     RPNIRYRIVAKDRAREQVLAFIRAEHAGEAGIIYCLSRRSVDETAAWLAGRGIHALPY
                     HAGLEAGVRRDHQQRFLREEGVVMVATVAFGMGIDKPNVRFVAHLDLPKSVEGYYQET
                     GRAGRDGLPADAWLSYGLNDAIQLRRMLAESGADERQKRVESLKLDSLLAYCETPRCR
                     RQVLLAYFGEQYPGPCGNCDTCLSPVETWDATVAAQKALSCVHRTGARFGVAHLIDVL
                     LGKATDKVAQHGHDKVSTFAIGSELGEAQWRSVYRQLVAAGLLAVDLEGYGALKLTNA
                     ARPVLRGETALHLRKDPPPNRRTREREPATGGPRRESPAQTLSAEQRVLWDALRQKRL
                     ELAREQGVPPYVIFHDATLLDFIVRAPRTLAELATVSGVGRSKLERYGETFLAVLLGH
                     EAGEAPAADAEAGDPAAQARSLLLRGAGVDEIAARLDVPRSQVLGWLAPLVRRGEAEL
                     DEVLPQLSVAELETIEAAFRSVDPAGWHDLKPIHAALGERHDFGTLACIRAALRRPDA
                     "
     gene            2246..2446
                     /locus_tag="PK144_10215"
     CDS             2246..2446
                     /locus_tag="PK144_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66144.1"
                     /translation="MHTLSTEQLLTWIRWYRRHRRTALARIPEDVPGTSGPDAAPTCE
                     PACEPADATPAVTRRQAGALKR"
     gene            complement(2455..3108)
                     /locus_tag="PK144_10220"
     CDS             complement(2455..3108)
                     /locus_tag="PK144_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNF66145.1"
                     /translation="MDQAVGLDVSCEPPWLQVDLGAPHRCLGWAVHRPGRVVARHVVW
                     LQVDEAELAPGVDPAALLGARLAARGLARAVGLMTARRVSAWEAGRAVVGDVGVDTVL
                     TLGLSNAVHVAGEAGPQPWRPGTINLLARLSVPLTDAALVEAVSIAAEARTAALLETA
                     GPAGGPLTGTGTDCIVIAVPPGPGRPYAGLHTATGQALGRAVYDTTRRAALAWQAQR"
     gene            3248..3814
                     /locus_tag="PK144_10225"
     CDS             3248..3814
                     /locus_tag="PK144_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNF66146.1"
                     /translation="MPVRQRLSIRTAGLCVWLGLAMMAALAPARAAPPGTLPVPVELP
                     APPPGPPVLTIHTPDGPRTYTRAGIEALGLLRVTTHTFWPADDGVYEGVPLARLLADA
                     GLAGAARIRVRALDEYSQAIPRADWAEHGLLLATRRDGRLLDIAGKGPLRLIYPRDSD
                     PALAGPGFRLRWVWMIESIEAEADAAAP"
     gene            3822..5828
                     /locus_tag="PK144_10230"
     CDS             3822..5828
                     /locus_tag="PK144_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66147.1"
                     /translation="MPAMNEARGHASAWVTRWPLLGVLFGLLLIAGGASWHVWRTTIG
                     IESQLPIASLENERDLGVLLQDVRSVRAQLELLLLRADESRLQAARLAIDVARTRARD
                     SRPLLLPDQYARQLDIVRAELERALDAFESGLGPPPDTERLSAARGPINRAAIASKQL
                     FDDVAGHSMMQLQAQGHTLARFRTSLAELLLFVLGASAGLAALILMLRKTLHARLAAE
                     RRLEHLAHHDPLTGLGNRTQFRMWLEDACLLAGQHGRSRRFALLLLDLDRFKDVNDSY
                     GHDAGDALLKAMAARISIAAGPRGRVARLGGDEFALLLPSSDTAAAGAVAETLVEALA
                     RPVAWAGLELYCGASVGLAACPEDGGGAQELVTNADLALYRAKAAGRSRWQGYTRELR
                     DALAVRRALESELRRALGSDEFELYYQPQLALASQRLAGVEALLRWRHPVRGLIPPGE
                     FIPLAEETGLIVPLGVLVLNLACRQIRAWREAGLTPPRVAVNLATAQFLNGNLADTVA
                     QLLAGYDIEHAALEFEVVERTLLDRNQGAVLEQLTALHAAGALIALDDFGTGYASLAH
                     LKLFPVHRLKIDRSFVADIHRDPGDEAIVRAIVQLGHAFGLEVIAEGVESVAQLEFLR
                     LHGCDLVQGYLYARPLPADEISAWVRDGRRPPEAQALPGRRSEG"
     gene            6012..6866
                     /locus_tag="PK144_10235"
     CDS             6012..6866
                     /locus_tag="PK144_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF66148.1"
                     /translation="MSENRFTGTERYVAPPELTLAVNAAATLGRPLLVKGEPGTGKTR
                     LAAEVAAALGAPLIEWHIKSTTKARQGLYEYDAVARLRDSQLGDPRVGDIGHYIVRGK
                     LWEAFACPVRPVLLIDEIDKADIEFPNDLLLELDRMEFHVYETRETVRAVHRPIIVIT
                     SNNEKELPDAFLRRCFFHYIRFPDRDTMIAIVAVHFPDLKKDLLAEALNVFFEIREMP
                     GLKKRPSTSELLDWLKLLLAEDIPPEALRAGEERRSIPPLHGALLKNEQDVHLFEKLI
                     FLHKRQGR"
     gene            6872..8065
                     /locus_tag="PK144_10240"
     CDS             6872..8065
                     /locus_tag="PK144_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF66149.1"
                     /translation="MLTGFIEALRANGLPIGLGEYLTLLDGLRARVTGPSVEDFYFYA
                     RLALIKDERLYDRYDRVFADYFGGIEAVFPPDPEHPRLAIPAEWLRRLSELHLSDEER
                     AQIEELGWDELMRRLIERLAEQRDAHHGGDHWIGTGGSSPYGHSGVNPAGVRLGGEGR
                     EGRAVKVWDRREYRNLDDTVELGTRNLKVALRKLRQFARSGAADTLDLDDTIESTARN
                     AGWLDLKLVPERHNAVKVLLLLDVGGSMDWHVRICEELFSAARAEFKHLKHYYFHNCV
                     YESVWIDNRRRRTERVPTAELLNTYGPDWRLVIVGDATMSPYEITQVNASVEHVNEEP
                     GAVWLQRLFAAWPRAVWLNPQPEAAWEQTMSLRLIQRLARQRMYPLTLAGIDRAIRSL
                     RSGEA"
     gene            8306..>9126
                     /locus_tag="PK144_10245"
     CDS             8306..>9126
                     /locus_tag="PK144_10245"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNF66150.1"
                     /translation="MSDPARSAVKAEAVRLDPSSDARGALAVITAAAARQIAANRSLL
                     VDGYDAEAVHQLRVGARRLRAALKLFGRYEPAVRRPPFLPALRTLVAAAGAARDPDVL
                     LDATLPRLAGGSDDPGAVAELARHYAGERDRAHRQLLEACADPASAALLIAIDGWISR
                     LPALGSRVLAEPVTGFAPRLLDRCARRLRHRGRHLLHLDAEARHAVRIAAKELRYAGD
                     GCASLYEAAAAEDWLHALAQLQDTLGELNDLAVLDARLRDAAPAGHGSAAARVRAL"
BASE COUNT         1274 a         3511 c         3117 g         1224 t
ORIGIN      
        1 cggcggcggc aagtcgctgt gctaccagat cccggcgctg ctgcgcgccg gcctcggcct
       61 cgtcgtctcg ccgctgatcg cgctgatgca cgatcaggtc gacgcgctga cccagctcgg
      121 cgtgcgcgcg gcgtacctga actccagcct cgatgccgcc agcgtgcgcg aaaccgagcg
      181 cgcattcgcc gccggcgaac tcgacctgct ctacgtcgcg cccgagcggc tggtcaccga
      241 gcgctgcctc ggcctgctcg cgcgcacgcc gctcgcgctg ttcgcgatcg acgaggcgca
      301 ctgcgtgtcg cagtggggcc acgacttccg gcccgagtac cgggcgctgt cggtgctgca
      361 cgagcgcttt ccggacgtgc cgcgcgtggc gctgaccgcg accgccgacg ccctgacgcg
      421 cgacgacatc cgcgagcacc tcggcctgct cgatgcgcgc gtgttcgtgt cgagcttcga
      481 ccggccgaac atccgctacc gcatcgtcgc caaggaccgc gcccgcgagc aggtgctcgc
      541 cttcatccgg gccgagcatg ccggcgaggc cggcatcatt tactgcctgt cgcgcaggag
      601 cgtcgacgaa accgccgcct ggctggccgg acgcggcatc cacgccctgc cctaccacgc
      661 cgggctggag gccggggtgc gccgcgatca ccagcagcgt tttttacggg aggaaggcgt
      721 ggtcatggtc gcgaccgtcg ccttcggcat gggcatcgac aagccgaacg tgcgcttcgt
      781 cgcgcacctc gacctgccga agtcggtcga gggctattac caggagaccg gccgcgccgg
      841 ccgcgacggc ctgccggccg acgcctggct cagctacggg ctcaacgacg cgatccagct
      901 gcggcgcatg ctcgccgaat ccggcgccga cgagcgccag aagcgcgtcg aatcgctgaa
      961 gctcgacagc ctgctcgcct actgcgagac cccgcgctgc cgccggcagg tgctgctcgc
     1021 gtacttcggc gagcaatacc ccggaccctg cggcaactgc gacacctgcc tgtcgccggt
     1081 cgagacctgg gatgcgaccg tcgccgcgca gaaggcgctg tcctgcgtgc accgcaccgg
     1141 cgcgcgcttc ggcgtcgcgc acctgatcga cgtgctgctc ggcaaagcca ccgacaaggt
     1201 cgcgcagcac ggccacgaca aggtcagcac cttcgccatc ggcagcgaac tgggcgaggc
     1261 gcagtggcgc tcggtctacc ggcagctggt cgccgccggc ctgctcgcgg tcgatctcga
     1321 aggctacggt gcgctgaagc tgacgaacgc cgcgcgaccg gtgctgcgcg gcgagacggc
     1381 gctgcacctg cgcaaggacc cgccgccgaa ccggcgcacg cgcgaacgcg aaccggccac
     1441 cggcggaccg cgccgcgaaa gcccggcgca gacgctgtca gcagagcagc gcgtgctgtg
     1501 ggacgcgctg cgccagaagc gcctcgaact ggcgcgcgag cagggcgtgc cgccgtacgt
     1561 gatcttccac gatgcgacgc tgctcgattt catcgtccgc gcaccgcgca cgctcgccga
     1621 actggcgacc gtctccggcg tcgggcgcag caagctcgaa cgctacggcg agaccttcct
     1681 ggccgtgctg ctcggacacg aggccggcga ggccccggct gccgacgccg aggccggcga
     1741 tccggccgcc caggcccgca gcctgctgct gcgcggtgcc ggcgtcgacg agatcgccgc
     1801 gcgcctcgat gtgccgcgca gccaggtgct cggctggctg gccccgctgg tgcgccgcgg
     1861 cgaggccgaa ctcgacgagg tgctgccgca gctgtcggtc gccgagctcg aaaccatcga
     1921 ggccgccttc cgcagcgtcg atccggccgg ctggcacgac ctgaagccga tccacgccgc
     1981 gctcggcgaa cgccacgatt tcggcacgct cgcctgcatc cgcgccgccc tgcgccgccc
     2041 ggacgcctga atcatccggc aaggcccgca gcccgagaac gcaggatcgc accgtgatct
     2101 gattcatcag cagccgtgca cctcctgcat cgcccgccgc gactgccatc ggctaccctg
     2161 tgcgtcccgg tccgccgtgc ccgatccctc tcgtcccccg cccgcctccc accccgaaga
     2221 ttccatttcc cgcaaggagc aacggatgca caccctgagc acggaacagc tgctgacctg
     2281 gatccgctgg tatcggcgtc atcgccgcac ggccttggcg cgcattcccg aggacgtgcc
     2341 cggcacgtcc ggacccgacg cggcgccgac ctgcgaaccg gcctgcgaac cggccgacgc
     2401 aacccccgcc gtgacccgcc gccaggccgg cgctttgaag cgctgagcgc cacctcagcg
     2461 ctgcgcctgc caggccagcg ccgcgcgccg ggtggtgtcg tagaccgccc ggcccagcgc
     2521 ctgaccggtc gcggtgtgca ggccggcgta cggccggccg ggacccggcg gcacggcgat
     2581 gacgatgcag tcggtgccgg tgccggtcag cgggccgccg gcgggaccgg cggtttcgag
     2641 cagcgccgcg gtgcgcgcct cggcggcgat cgacaccgcc tcgaccagcg ccgcgtcggt
     2701 gagcggcacc gacagccgcg ccagcagatt gatcgtgccc ggccgccacg gctgcgggcc
     2761 ggcctcgccg gcgacgtgga ccgcgttcga cagaccgagc gtcagcaccg tatcgacccc
     2821 cacatccccg accaccgccc gcccggcctc ccaggcgctg acccggcgcg cagtcatcag
     2881 gccgacggcc cgcgccagcc cgcgcgcggc cagccgcgca cccagcagcg ccgccggatc
     2941 gacgccgggg gcgagttccg cctcgtcgac ctgcagccag accacgtgcc gggcgacgac
     3001 gcggcccggc cggtgcacgg cccagcccag acagcggtgc ggcgccccga gatcgacctg
     3061 cagccacggc ggctcgcagc tcacgtccag tccgacggcc tgatccatgc cggcctcgcc
     3121 ccctgcgcct gcgctccgcg gtattgcggc cgcgccattg tcacggaacg gccgaccgca
     3181 cacactcccg gcacccgatc cccgccgctg cgcgttatct cttctcccgg agtcgagccc
     3241 gccgctcatg cccgtccgcc agcgtctttc gatccgcacc gccggcctgt gcgtctggct
     3301 cggcctcgcc atgatggccg ccctggcgcc tgcacgcgcc gcgccgcccg gcacgctgcc
     3361 ggtgccggtc gagctgccgg ccccgccgcc ggggccgccg gtgctgacga tccacacgcc
     3421 ggacggcccg cgcacctata cccgcgccgg catcgaggcg ctcggcctgc tgcgcgtgac
     3481 cacgcacacg ttctggcccg cggacgatgg cgtctacgaa ggcgtgccgc tcgcgcggct
     3541 gctcgccgat gccggactcg ccggggcggc gcgcatccgc gtgcgggcgc tcgatgaata
     3601 ctcgcaggcg atcccgcggg ccgactgggc cgaacacggg ctgctgctgg ccacgcgccg
     3661 cgacggccgc ctgctcgaca tcgccggcaa gggcccgctg cggctgatct atccgcgcga
     3721 cagcgacccg gcgctcgccg gccccggctt ccggctgcgc tgggtgtgga tgatcgagag
     3781 catcgaagcc gaagcggacg ctgccgcgcc gtgaacctcc catgcctgcc atgaatgaag
     3841 ctcgcgggca cgcttccgcc tgggtgacgc gctggccgct gctcggcgtg ctgttcggcc
     3901 tgctgctgat cgccggcggc gcgagctggc acgtctggcg cacgacgatc ggcatcgaga
     3961 gccagctgcc gatcgccagc ctcgagaacg aacgcgatct cggcgtgctg ctgcaggacg
     4021 tgcgctcggt gcgtgcgcag cttgagctgc tgctgctgcg cgccgacgag tcccgcctgc
     4081 aggcggcccg cctggccatc gatgtcgccc gcacccgcgc ccgcgacagc cgtccgctgc
     4141 tgctgccgga tcagtacgca cgccagctcg acatcgtgcg cgccgaactc gaacgcgcgc
     4201 tcgatgcctt tgaatcggga ctgggccccc cgcccgacac cgagcgcctg agcgccgcgc
     4261 gcgggccgat caaccgcgcc gcgatcgcca gcaagcagct tttcgatgac gtggccgggc
     4321 attcgatgat gcagctgcag gcgcagggcc atacgctcgc gcgctttcgc accagcctcg
     4381 ccgaactgct gctgttcgtg ctcggcgcca gcgccggcct ggccgccctg atcctgatgc
     4441 tgcgcaagac cctgcacgcg cgccttgccg ccgaacgccg gctcgaacac ctcgcccacc
     4501 acgatccgct gaccgggctc ggcaaccgca cgcagttccg gatgtggctg gaggatgcct
     4561 gcctgctcgc cggccagcac ggccgcagcc ggcgcttcgc gctgctgctg ctcgatctcg
     4621 accgtttcaa ggacgtgaac gacagctacg gccacgacgc cggcgacgcg ctgctgaagg
     4681 ccatggccgc ccgcatctcg atcgccgccg gcccgcgcgg ccgggtcgcg cgcctcggcg
     4741 gcgacgaatt cgcgctgctg ctgccgagca gcgacaccgc ggccgccggt gccgtcgccg
     4801 agacgctggt cgaggcgctg gcgcggccgg tggcctgggc cgggctcgaa ctgtactgcg
     4861 gtgccagcgt cgggctcgcg gcctgtcccg aggacggcgg cggcgcgcag gagctcgtca
     4921 ccaatgccga tctggcgctg taccgcgcca aggccgccgg ccgcagccgc tggcagggct
     4981 atacgcgcga gctgcgcgac gcgctcgccg tgcgccgggc gctggaatcc gagctgcgcc
     5041 gcgcgctcgg cagcgacgag ttcgagctgt actaccagcc gcagctcgcc ctcgccagcc
     5101 agcgcctcgc cggcgtggag gcgctgctgc gctggcgcca cccggttcgc ggcctgatcc
     5161 cgcccggcga gttcatcccg ctcgccgagg aaaccgggct gatcgtgccg ctcggcgtgc
     5221 tggtgctgaa cctcgcctgc cgccagatcc gcgcctggcg cgaggcgggc ctgacgccgc
     5281 cgcgcgtggc cgtgaacctc gcgaccgcgc agttcctgaa cggcaacctc gccgacacgg
     5341 tcgcgcagct gctcgccggc tacgacatcg agcacgcggc gctcgagttc gaggtggtcg
     5401 agcgcacgct gctcgaccgc aaccagggcg cggtgctcga acagctcacg gcgctgcacg
     5461 ccgccggcgc gctgatcgcg ctcgatgact tcggcaccgg ctacgcctcg ctcgcgcacc
     5521 tgaagctgtt cccggtgcac cggctgaaga tcgaccgcag cttcgtcgcc gacatccacc
     5581 gcgaccccgg cgacgaggcc atcgtgcgcg ccatcgtcca gctcggccac gccttcgggc
     5641 tcgaagtcat cgccgagggc gtcgagagcg tcgcgcagct cgaattcctg cgcctgcacg
     5701 gctgcgatct ggtgcagggc tacctgtacg cgcggccgct gccggccgac gagatcagcg
     5761 cctgggtgcg cgacggccgc cggccccccg aggcacaggc actgccgggg cggcgcagcg
     5821 agggctgaga ggccgaagcc ggggccgcgc gccccgcacc ggcgcgcgga ctccttcgac
     5881 cgcttccggc cggcctgcac ctgccatgca cgccggtccg gcacatctgc cgtgcttgcc
     5941 gtgcgcaccg gcccgcggtc catactgcgc cggcccgttt ccccgcgtgc ccgacgagga
     6001 gaggatccgt aatgagcgaa aaccgcttca ccggcaccga gcgctacgtc gcgccgcccg
     6061 agctgacgct cgccgtcaac gccgccgcca cgctcgggcg gccgctgctg gtcaagggcg
     6121 agcccggcac cggcaagacg cggctcgccg ccgaggtcgc cgccgcgctc ggtgcgccgc
     6181 tgatcgagtg gcacatcaag agcacgacca aggcccgcca ggggctgtat gaatacgacg
     6241 cggtcgcgcg cctgcgcgat tcgcagctcg gcgatccgcg cgtcggcgac atcggccact
     6301 acatcgtgcg cggcaagctc tgggaggcct tcgcctgccc ggtgcggccg gtgctgctga
     6361 tcgacgagat cgacaaggcc gacatcgaat tcccgaacga cctgctgctc gaactcgacc
     6421 gcatggagtt ccacgtctac gagacgcgcg agaccgtgcg cgccgtgcac cggccgatca
     6481 tcgtcatcac ctcgaacaac gagaaggaac tgccggacgc cttcctgcgc cgctgcttct
     6541 tccactacat ccgcttcccg gaccgcgaca cgatgatcgc catcgtcgcc gtgcacttcc
     6601 cggacctgaa gaaggacctg ctcgccgagg cgctgaacgt gttcttcgag atccgcgaga
     6661 tgccgggcct gaagaagcgg ccgtcgacct cggagctgct cgactggctg aagctgctgc
     6721 tcgccgagga catcccgccc gaggcgctgc gcgccggcga ggagcggcgc agcatcccgc
     6781 cgctgcacgg cgcgctgctg aagaacgagc aggacgtgca cctgttcgag aagctgatct
     6841 tcctgcacaa gcgccaggga cgctgagcgc cgtgctgacc ggcttcatcg aggcgctgcg
     6901 cgcaaacggc ctgccgatcg ggctcggcga gtacctgacg ctgctcgacg gtctgcgcgc
     6961 gcgcgtgacc gggccgtcgg tcgaggattt ctacttctac gcgcgcctcg cgctgatcaa
     7021 ggacgagcgc ctctacgacc gctacgaccg cgtgttcgcc gactacttcg gcggcatcga
     7081 agcggtcttc ccgcccgacc ccgaacaccc gcggctcgcg atcccggccg agtggctgcg
     7141 ccggctctcc gagctgcacc tgagcgacga ggagcgtgcg cagatcgagg aactcggctg
     7201 ggacgagctg atgcgccgcc tcatcgagcg cctcgccgag cagcgcgacg cgcaccacgg
     7261 cggcgaccat tggatcggca ccggcggcag ctcgccgtac gggcactccg gcgtcaaccc
     7321 ggccggcgtg cgcctcggcg gcgagggccg cgaaggccgg gcggtgaagg tctgggaccg
     7381 gcgcgagtac cgcaacctcg acgacacggt cgagctcggc acgcgcaacc tcaaggtggc
     7441 gctgcgcaag ctgcgccagt tcgcgcgcag cggcgccgcc gacacgctcg atctcgacga
     7501 caccatcgaa tcgaccgcgc gcaacgccgg ctggctggac ctgaagctgg tgccggagcg
     7561 ccacaacgcc gtgaaggtgc tgctgctgct cgatgtcggc ggctcgatgg actggcacgt
     7621 gcgcatctgc gaggagctgt tctcggcggc gcgcgccgag ttcaagcacc tgaagcatta
     7681 ctacttccac aactgcgtct acgagagcgt ctggatcgac aaccgccgcc ggcgcaccga
     7741 gcgcgtgccg acggccgagc tgctgaacac ctacgggccg gactggcggc tggtgatcgt
     7801 cggcgatgcg acgatgagcc cctacgagat cacgcaggtc aacgccagcg tcgagcacgt
     7861 caacgaggag cccggtgccg tctggctgca gcggctgttc gcggcctggc cgcgagcggt
     7921 gtggctgaac ccgcagccgg aagcggcctg ggaacagacg atgtcgctgc ggctgatcca
     7981 gcgcctcgcc cgccagcgca tgtacccgct gacgctggcc gggatcgatc gcgcgatccg
     8041 ctcgctgcgc agcggcgagg cctgatcggc atgggcggta tcaggaaacc ggcaaaaaaa
     8101 gaacccgtcc ggagtgcgcc ccggacgggt gacgagttgc gacttctcga atgcggtctg
     8161 atcgacctgt ggctgccgga tccctgagcg ccgctgccgt gtccgttcgg caaaggggct
     8221 cgatcacgag ttccgggagc ctagcgcggt accgggcgcg cgccagtgcc cggccgctaa
     8281 atccacaagt tctcacagga atttaatgtc cgaccccgcc cgcagcgccg tcaaggccga
     8341 agccgtccgg ctcgatccgt ccagcgatgc ccgcggcgcg ctcgccgtga tcaccgccgc
     8401 cgccgcccgc cagatcgcgg ccaatcgcag cctgctcgtc gacggctacg acgccgaagc
     8461 cgtgcatcag ctgcgcgtcg gcgcccgccg cctgcgggcg gccctgaagc tgttcggccg
     8521 ctacgagccc gccgtgcgcc gcccgccgtt cctgccggcg ctgcgcacgc tggtggccgc
     8581 cgccggtgcg gcgcgcgatc ccgatgtgct gctcgatgcg acgctgccgc ggctggccgg
     8641 cggcagcgac gatccgggcg cggtcgccga actcgcgcgc cactacgccg gcgagcgtga
     8701 ccgcgcgcac cggcagctgc tcgaagcctg cgccgatccg gcctctgcgg cgctgctgat
     8761 cgcgatcgac ggctggatca gccggctgcc ggcgctcggg agccgggtcc tggccgagcc
     8821 ggtcaccggc tttgcaccgc ggctgctcga tcgctgcgcc cggcggctgc gccaccgcgg
     8881 ccgtcacctg ctgcacctcg atgccgaagc ccgccacgcc gtgcgcatcg ccgccaagga
     8941 actgcgctac gccggcgacg gctgcgcgtc gctgtacgaa gcggcggcgg cagaggactg
     9001 gctgcatgcg ctcgcgcagc tgcaggacac gctcggcgag ctcaacgacc tcgccgtgct
     9061 cgatgcgcgc ctgcgcgacg ccgctccggc cggccacggc agcgcagccg cccgcgtgcg
     9121 ggccct
//
LOCUS       DAOQIE010000147         9118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3854_length_9118_cov_6.867814, whole genome shotgun sequence.
ACCESSION   DAOQIE010000147 DAOQIE010000000
VERSION     DAOQIE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3854_length_9118_cov_6.867814"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    14..113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(405..1214)
                     /locus_tag="PK144_10250"
     CDS             complement(405..1214)
                     /locus_tag="PK144_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF66151.1"
                     /translation="MSLPPPSAPSRRTLALLAAAALALGWLLLDTGVGPGRWIEVNGH
                     ELYYETRGYGPPLLLLHGGGDSGAGSFSRQFDDFTAAHRLIAPDQIGQGRTPDVPGPL
                     SYTAMMNDTIALLDQLRPGPVDVVGYSDGGILALMLAVRRPDLVRRLVVSGANLSPDG
                     LTAKELAELRTAVARARGRSDPTLGDRLNTLWLHSPTPEEISPALLAGLDKPVLVMSG
                     DRDAITLEHTLAIYRALPKAELCILPGTGHGTFGARPQWVNPIILDFLGRS"
     gene            complement(1268..1714)
                     /locus_tag="PK144_10255"
     CDS             complement(1268..1714)
                     /locus_tag="PK144_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF66152.1"
                     /translation="MNAVANRLYLRLARPGDADAIAGLMTLLGYPVEAGETAGRLERL
                     LRHPDAAVLVGGLEDGPVQGCLALHFIPQLGLAGDFCRIAYFSVAPAAQGQGLGRALE
                     AEATRLAQQRGCDRIEVHSHERRHGAHVFYRRRGYEESPRYLIRRP"
     gene            complement(1711..2040)
                     /locus_tag="PK144_10260"
     CDS             complement(1711..2040)
                     /locus_tag="PK144_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNF66153.1"
                     /translation="MITRIRQGRTRPGQAGEYEPLLLEHVFPEIAARALSGWPGYELL
                     RRERSHEVEFVTIMHFDDQAALHTFDHLDGSAEGVPPEARTLLAHFDSLSSHYRTVAV
                     RAGGHTT"
     gene            complement(2126..2656)
                     /locus_tag="PK144_10265"
     CDS             complement(2126..2656)
                     /locus_tag="PK144_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66154.1"
                     /translation="MSTDPLLTSDFAEALLATQTGPQAPATLRFDATRTGLAFGGTVP
                     AVRVYAFGPASLARHWHPGFPTPAQLEYAIAAVEDELMRVHRHCGPPPSLASAVCPDP
                     EPRALAASLGLPGSGRVQLLREAVEHGFGRFAACAEGRPSDSGGLPQDTNGMALLLIL
                     RELMHHLPLAALELPA"
     gene            complement(2729..3565)
                     /locus_tag="PK144_10270"
     CDS             complement(2729..3565)
                     /locus_tag="PK144_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003147081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF66155.1"
                     /translation="MSNSGHLAPALTPRPADAPERSVPALERLPRAVYARAESLPAGS
                     WVRPHRHDWVQLSWALSGVLEVRTDTGCWLAPPQRAVWIPPGTLHEVNASARAEMRSL
                     YLDASRTAWAPPRCRVLAVSPLVRELILALSARPLEYDPDGPAGRLAEVLLDAIAELP
                     ELDFGLPLPADARLRRLCEALQAEPADARTLPEWAARCGLSERTLTRRFRRETGLSFA
                     DWRRRLRLLLALGALEAGRSVTAVALDSGYDSLSAFIAAFRNEFGTTPKALFAPAPVN
                     DG"
     gene            3666..4655
                     /locus_tag="PK144_10275"
     CDS             3666..4655
                     /locus_tag="PK144_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNF66156.1"
                     /translation="MLLPHLRRLLRDRFLLGMLLAVGLAVLTPQFGRSGGTLHLDALS
                     NAGIFAVFFLHGIGLSMQSLRAGLLNWRLHTLVQAHTFLVFPLLWLALDALFGAWVPQ
                     GLMLGFWYLCALPSTISSSVAMTALGRGNVPGAIFNATLSSLLGIVLTPLLVSLMVAH
                     GGEGGRGPGEAILNIAGLLLAPFVLGQLLRPWLGAWFAERKRWTGLIDRGVILMLVYG
                     SFCDATAGGLWSDYGAGVLATAFAGSALLLAIVLGLSRRAARLFGFSVEDEVAAVFCG
                     SKKTLASGMPMAKLLFGASPALGLVVLPILLYHPLQLFVCSVMAERYAARPAR"
     gene            complement(4659..5330)
                     /locus_tag="PK144_10280"
     CDS             complement(4659..5330)
                     /locus_tag="PK144_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNF66157.1"
                     /translation="MSPPPAPVLVIGNKNYSSWSLRPWLLLKQLGIAFTEVRLPLDTP
                     QFVTEIGRWSPSGRVPVLHHGELVVWDSLAIAEYVNEVLAPGRVWPKALEARAIARSV
                     AAEMHSGFGALRSELPMNIRAHRRVEPSVDCNADIARLQALIRESRLLHGADGPWLFG
                     EFSAADAMLAPVAWRFTTYDVPHDDIVGAWLSTVLECEAMRAWANDARTETEIIAADE
                     AGIPV"
     gene            complement(5454..6101)
                     /locus_tag="PK144_10285"
     CDS             complement(5454..6101)
                     /locus_tag="PK144_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNF66158.1"
                     /translation="MSQITLVTGTRNNSPWSLRPWLLMRQLGIPFDEIRLPIGARERL
                     PDGGRWSPESGIPVLKHGALTVRDSLAIAEYLNEALAPGRVWPADLASRAVARSVSAE
                     IHSNFSVFRRELPMDVAGRHSPVLSADCRADIAQVQSVIRDCRLRHGRGGPWLFGEFS
                     AADAMYAPVAVRFRSYGIATDSAVAGWVRALLELPAMIEWIDDARDESAITCAVA"
     gene            complement(6462..7787)
                     /locus_tag="PK144_10290"
     CDS             complement(6462..7787)
                     /locus_tag="PK144_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66159.1"
                     /translation="MSALPVTELRRARPAAGLLASMLVLTLASAFVPALTPWPAAACA
                     WSAAALLWPRLPQRQHRQALGMAGIGVAGMLAGVLSGVPADLRLAATGNQTLIGLLCA
                     VSFLRLVTMPDAQTRSEALPRGRTALWRTLLGVHLFGAVINIPAVFILADRLLRNGRL
                     GRRAALVLTRGFCACTFWSPFFAAMAAALNFAPGARLGIIILTGLPLAALALWLTALD
                     TDDGRRDTRGAGFVGYPMHLRTLAVPGTLVGLVLGTHLLWPQTGITTIVTLYAPLLCL
                     VVLLGRRGRAGLHAFAGHALRRLGDGAPELVLFLAAGLLAAGITGLLAATGGQLPFVR
                     FGPIEASLTLLLIVAAAFVGVHPVIAITTFGTWLAPLAPDPTLLTSTLTMGWAIGVPA
                     GPLSGLHLSMQGRYGIPVFAFQRWNNTYIPKLLAASILALHLQAALRGL"
     gene            7925..8632
                     /locus_tag="PK144_10295"
     CDS             7925..8632
                     /locus_tag="PK144_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF66160.1"
                     /translation="MPYIPTQNAWNGEADCRTCSVRGSVLFAGLQQNDFDLIHQPIDD
                     IAVPAQGRVYHVHEPGARVFTLRSGLVKLVQYLPDGSQRIVRLLRGNDVLGLEALLGQ
                     PYQHDAIALTAVELCAIPREVVAVLAREQPALHTELLKRWQQALSDADVWLTELSTGT
                     ARARVARLLLRLAGPRPVEAFRLLGREDIGAMLGLTPETVSRTVAELRRQKLLREQGA
                     GLFTVDAPALRRIADGG"
     gene            8684..>9118
                     /locus_tag="PK144_10300"
     CDS             8684..>9118
                     /locus_tag="PK144_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNF66161.1"
                     /translation="MTGIRALLFDVFGTLVDWRTSVAREAEAVLAPLGHALDWPAFAD
                     DWRARYQPAMEAVRCGLEPYVALDELHRRNLDAVLASRGIELDEARCRHLNLAWHRLD
                     AWPDVGDGLAALRAPFLLAPCSNGNIALMVALARRNGWHWDAI"
BASE COUNT         1311 a         3061 c         3364 g         1282 t
ORIGIN      
        1 tccccggtcg tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagcgc
      121 atccccagtc gtaccgagcg catccccagt cgtaccgagc gcatccccag tcgtaccgag
      181 cgcatccccg gtcgtaccga gcgcatcccc agtcgtaccg agcgcatccc cagtcgtacg
      241 gagcgcatcc ccggtcgtat ggagcgcgtc cccggtcatg cgcagcgcat ccccggcgcg
      301 gtgcggcgca ttgatcgcga gggggacagg aagagcggcg gggtccggcg gggggcggtg
      361 ccggtgccgg ggcgtcgtgc ggcaggcgtg ccgggccggc ggcttcagct gcgtccgagg
      421 aaatcgagga tgatcgggtt cacccactgc ggccgcgcgc cgaaggtgcc gtggccggtg
      481 ccgggcagga tgcagagttc ggccttcggc agcgcgcggt agatcgccag cgtgtgttcg
      541 agcgtgatcg catcgcgatc gccggacatc accagcaccg gcttgtcgag gccggcgagc
      601 agcgccgggc tgatttcctc cggggtcggc gaatgcagcc acagcgtgtt caggcgatcg
      661 ccgagcgtcg gatcgctgcg gccgcgggcg cgggcgaccg cggtgcgcag ttcggcgagt
      721 tccttcgcgg tcagcccgtc cggcgacaga ttggcgccgc tgaccacgag gcggcgcacc
      781 agatcgggcc ggcgcacggc cagcatcagc gcgaggatgc cgccatcgct gtagccgacc
      841 acatcgaccg ggccgggtct gagctgatcg agcagcgcga tggtgtcgtt catcatcgcc
      901 gtgtagctga gcggccccgg cacgtcgggc gtgcggccct gaccgatctg gtccggggcg
      961 atcaggcgat gcgcggcggt gaaatcatcg aactggcgcg agaacgagcc ggcaccggag
     1021 tcgccgccgc cgtgcagcag cagcagcggc gggccgtagc cgcgggtttc gtagtacagc
     1081 tcgtggccgt tgacctcgat ccagcgcccc ggaccgaccc cggtgtcgag cagcagccag
     1141 ccgagcgcca gcgcggccgc ggcgagcagc gccagcgtgc gtcgcgacgg cgccgacggg
     1201 ggaggcaggc tcatgaaggg cggggcggcg cgtcactgca ctcggcgacg cgcgggctgt
     1261 cggcgggtca gggacggcgg atcaggtagc gcggcgattc ctcgtagccg cgccggcggt
     1321 agaacacgtg cgcgccgtgg cggcgctcgt ggctgtgcac ctcgatgcgg tcgcagccgc
     1381 gctgctgcgc gaggcgcgtg gcctcggcct ccagcgcccg gccgagtccc tgtccctgcg
     1441 cggccggggc gacgctgaag taggcgatgc ggcagaagtc gccggccagg ccgagctgcg
     1501 gaatgaagtg cagcgccaga cagccctgca ccgggccgtc ctcgaggccg ccgaccagca
     1561 ccgcggcatc gggatgacgc agcagccgtt cgagccgccc ggcggtctcg ccggcttcga
     1621 ccggatagcc gagcagcgtc atcaggccgg cgatcgcatc ggcatcgccc ggccgggcga
     1681 ggcgcaggta cagacggttg gcgacggcgt tcatgtggtg tgacctccgg cacggaccgc
     1741 gacggtccgg tagtgcgagg acagggaatc gaagtgcgcg agcagcgtcc gggcttcggg
     1801 tggcacgccc tcggctgatc catcgaggtg atcgaaggtg tggagtgcgg cctgatcgtc
     1861 gaaatgcatg atggtcacga actcgacctc atggctgcgc tcgcggcgca gcagctcgta
     1921 gccgggccag cctgacaggg cgcgggcggc gatctccgga aacacgtgtt cgagcagcag
     1981 aggttcgtac tcgccggcct gcccgggacg ggtccggcct tgccggatgc gcgtgatcat
     2041 caagggagtc cgtccgcaaa gctaaagatt tggacagaaa ctagacgacg ccgtgatccg
     2101 gctcaatccc acgatttctg tgtcttcagg ccggcagttc gagggcggcg agcggcaggt
     2161 ggtgcatcag ttcgcgcagg atcagcagca gtgccatgcc gttcgtgtcc tgcggcaggc
     2221 cgccgctgtc ggacggccgg ccttcggcgc aggcggcgaa gcgcccgaag ccgtgttcga
     2281 cggcctcgcg cagcagttgc acgcggccgc tgcccggcag gccgaggctc gcggccagtg
     2341 cccgtggctc cgggtccggg cagacggcgg acgccagcga cggcggcggc ccgcagtggc
     2401 ggtgcacgcg catcagttcg tcctcgacgg cggcgatcgc gtattccagc tgcgcgggcg
     2461 tcgggaagcc ggggtgccag tgacgggcga ggctggccgg cccgaaggca tagacgcgta
     2521 ccgccggcac ggtgccgccg aaggccaggc cggtgcgggt ggcgtcgaag cgcagcgtcg
     2581 ccggcgcctg cggcccggtc tgcgtggcga gcagggcttc ggcgaagtcg gaggtcagga
     2641 gaggatcggt gctcatgcgc ggctccctgc ggaggcacgg ccgggcaggg tcatctgccg
     2701 cgccatgcgg gaaatcatca gtcgcaaatc atccatcgtt caccggtgcc ggtgcgaaca
     2761 gcgccttcgg cgtcgtgccg aactcgttgc ggaaggcagc gatgaaggcg gaaagcgaat
     2821 cgtagccgct gtcgagcgcg accgcggtga cgctgcgccc ggcttcgagc gcgccgaggg
     2881 cgagcagcag gcgcaggcgc cggcgccagt cggcgaagct caggccggtc tcgcggcgga
     2941 agcgccgcgt cagcgtgcgc tcggacaggc cgcagcgcgc cgcccattcg ggcagcgtgc
     3001 gtgcatcggc cggttcggcc tgcagggctt cgcagagccg gcgcaggcgc gcatcggccg
     3061 gcagcggcag gccgaagtcg agttcgggca gctcggcgat ggcatcgagc agcacttccg
     3121 ccagccgtcc ggccggtccg tcggggtcgt attcgagcgg ccgggcgctc agtgcgagga
     3181 tcagttcgcg caccagcggg ctcacggcca gcacccggca gcgcggcggg gcccaggcag
     3241 tgcgtgacgc atcaagataa aggctgcgca tctcggcacg cgccgaggca ttgacctcgt
     3301 gcagcgtgcc gggcgggatc cacaccgcgc gctgcggcgg tgccagccag cagccggtgt
     3361 cggtgcgcac ttcgagcacg ccgctgagcg cccacgacaa ctgcacccag tcatggcgat
     3421 gcggacgcac ccaggagccg gccggcagcg attcggcgcg tgcgtagacg gcgcgcggca
     3481 ggcgttcgag cgccggcacg ctgcgctcag gcgcatcggc gggccgtggc gtcagggcgg
     3541 gcgcgagatg gccggaattc gacatcgatt gaccccgcgg cgacgaccgg acaggccgtt
     3601 cctgcgcagt atgcccggca gacggtcgcg ttcccggcga ccgtaccggt gccggagatt
     3661 ccgccatgct gctgcctcac ctgcgtcgcc tgctgcgcga ccgtttcctg ctcggcatgc
     3721 tgctcgccgt cggactcgcg gtgctgacgc cgcaattcgg gcgcagcggc ggcacgctgc
     3781 acctcgatgc gctctcgaat gccggcatct ttgcggtgtt cttcctgcac ggcatcgggc
     3841 tgtcgatgca gagcctgcgc gcgggcttgc tgaactggcg gctgcacacg ctggtgcagg
     3901 cgcatacctt cctcgtcttc ccgctgctgt ggctagcact cgatgcgctg ttcggcgcct
     3961 gggtgccgca ggggctgatg ctcggcttct ggtatctgtg cgcgctgccc tcgacgatct
     4021 cctcgtcggt ggcgatgacc gcgctcgggc gcggcaacgt gccgggggcg atcttcaacg
     4081 ccacgctgtc gagcctgctc ggcatcgtgc tgacgccgct gctggtcagc ctgatggtcg
     4141 ctcacggcgg ggagggcggg cgcggccccg gcgaggcgat cctgaacatc gccggcctgc
     4201 tgctcgcgcc cttcgtgctc gggcagctgc tgaggccgtg gctcggcgcc tggtttgcag
     4261 agcgcaagcg ctggacgggc ctgatcgacc gcggcgtgat cctgatgctg gtctacggct
     4321 cgttctgcga cgcgacggcc ggcgggctgt ggtcggacta cggcgccggc gtgctcgcga
     4381 cggcgtttgc cggcagtgcg ctgctgctgg cgatcgtgct ggggctgagc cggcgcgctg
     4441 cacgcctgtt cggcttttcg gtcgaggacg aggtggcggc ggtgttctgc ggctcgaaga
     4501 agacgctggc ctcgggcatg ccgatggcga agctgctgtt cggcgccagc cccgcgctcg
     4561 ggctggtggt gctgccgatc ctgctctatc acccgctgca gctgttcgtc tgctcggtga
     4621 tggccgaacg ctacgcggcc cggccggcac gttgagcttc agaccggtat gccggcctcg
     4681 tcggcggcga tgatctcggt ttcggtgcgc gcgtcgttcg cccaggcgcg catcgcctcg
     4741 cattcgagca ccgtcgacag ccaggcaccg acgatgtcgt cgtgcggcac gtcgtaggtc
     4801 gtgaagcgcc aggcgaccgg cgcgagcatc gcatcggcgg ccgagaactc gccgaacagc
     4861 cacgggccgt cggcgccgtg cagcagccgg ctttcgcgga tcagcgcctg cagccgcgcg
     4921 atgtcggcgt tacagtcgac cgagggctcg acgcgccggt gcgcgcggat gttcatcggc
     4981 agctcgctgc gcagcgcccc gaagcccgag tgcatctcgg cagccaccga acgggcaatg
     5041 gcgcgggctt cgagcgcctt cggccagacg cggccggggg cgaggacttc gttgacgtac
     5101 tcggcgatgg cgagcgaatc ccagaccacc agctccccgt ggtgcagcac cggcacgcgc
     5161 ccgctcggac tccagcggcc gatctcggtg acgaactgcg gggtgtcgag cggcaggcgg
     5221 acctcggtga aggcgatgcc gagctgcttc agcagcagcc acggccgcag cgaccacgag
     5281 gagtagttct tgttgccgat gacgaggacg ggagcggggg gcggagacat ggcagacagc
     5341 accggtacgc ttgcgcccgt cgggcgcggg agcctgccag catagccgac accgcacccc
     5401 gcacccgtca gcgaagcttt ccggaagtca agatttgtgc aggccggccg gtttcaggcc
     5461 accgcgcagg tgatggccga ttcgtcgcgg gcatcgtcga tccattcgat catcgccggc
     5521 agttcgagca gggcgcggac ccagcccgcg acggcgctgt cggtggcgat gccgtagctg
     5581 cggaaacgca cggcgaccgg cgcgtacatc gcatcggcgg ccgagaactc gccgaacagc
     5641 cagggtccgc cgcgaccatg gcgcaggcgg cagtcccgga tcaccgactg cacctgcgcg
     5701 atgtcggcac ggcagtcggc cgacagcacc gggctgtggc gcccggcgac atccatcggc
     5761 agctcgcgcc ggaacaccga gaagttgctg tggatctctg cggacaccga gcgggcgacg
     5821 gcacggctgg cgagatcggc gggccacacg cggccggggg cgagggcctc gttcaggtat
     5881 tcggcgatcg ccagcgaatc gcgcacggtc agcgcgccgt gtttcaggac ggggatgccg
     5941 gactccggac tccagcgacc gccatccggg agccgctcgc gcgcaccgat cggcaggcgg
     6001 atctcatcga aggggatgcc gagctggcgc atcagcagcc agggacgcag cgaccacggg
     6061 gagttgttcc gggtgccggt gacgagcgtg atctgactca tggggcggtc tccctgggtt
     6121 gttgttagat cgctgtcctt gcgcgacacg gctgtatccg gatgcagacg tatcgggatg
     6181 attcaggaat ttccggaccg gcgcaaaccg tcagcgagtg tggaatttta aggtgtgcgc
     6241 cagcgccccg ttgccgccgc cacccctgct gaccacctcg tcggcccgga tcgggcattc
     6301 ctgccgccgg gccagcgacc agtccggcgg tcggttgata acgtgctgaa tgttacagga
     6361 tttgtcaaag tatttgtcat aacgagtttg taaggtccgg gcgtgcttga caagcgcggc
     6421 tcgcacccgg tttgtaaccg tccgccctcc cgcggcagcc gtcacagacc gcgcagggcg
     6481 gcctgcaggt gcagcgcgag gatcgatgcg gccagcagct tcgggatgta agtgttgttc
     6541 cagcgctgga acgcaaacac cgggatgccg tagcgcccct gcatcgacag gtgcagcccc
     6601 gacagcggtc cggccggcac gccgatcgcc cagcccatcg tcagcgtgct ggtcagcagc
     6661 gtcgggtccg gcgcgagcgg cgcgagccag gtgccgaagg tcgtgatcgc gatcaccgga
     6721 tgcacgccga cgaaggccgc cgcgacgatc agcagcagcg tcaggcttgc ctcgatcggc
     6781 ccgaagcgca cgaacggcag ctgcccgccg gtggcggcga gcagcccggt gatgccggcg
     6841 gcgagcaggc cggcggcgag gaacagcacc agttccggcg cgccgtcgcc gaggcggcgc
     6901 agcgcgtgcc cggcgaaagc gtgcagcccc gcgcggccgc ggcgcccgag cagcacgacg
     6961 aggcagagca gcggcgcata cagcgtgacg atggtcgtga tgccggtctg cggccacagc
     7021 aggtgcgtcc cgagcaccag cccgaccagc gtccccggca ccgccagcgt gcgcaggtgc
     7081 atcggatagc cgacgaagcc cgcaccgcgc gtgtcgcggc ggccgtcgtc ggtgtcgagt
     7141 gccgtcagcc acagcgccag ggctgccagc ggcaggccgg tgaggatgat gatgccgagc
     7201 cgcgccccgg gcgcgaagtt cagcgccgcc gccatcgcgg cgaagaacgg tgaccagaac
     7261 gtgcaggcgc agaagccgcg cgtcagcacc agcgccgcgc gccggccgag gcggccgttg
     7321 cgcagcagcc ggtcggcgag gatgaacacc gccgggatgt tgatgacggc gccgaacaga
     7381 tgcacgccga gcagcgtgcg ccagagcgcc gtgcgcccgc gcggcagcgc ttcgctgcgg
     7441 gtctgtgcgt ccggcatcgt caccagccgc aggaaggaca cggcgcagag cagtccgatc
     7501 agcgtctggt tgccggtggc ggcgaggcgc agatccgcag gcacgccgga cagcacgcct
     7561 gccagcatgc cggcaacgcc gatgccggcc atgccaagcg cctgccggtg ctggcgctgc
     7621 ggcaggcgcg gccacagcag ggcggcggcg ctccaggcgc aggcggcggc cggccacggc
     7681 gtcagcgccg gcacgaaggc cgaggcgagc gtcagcacga gcatcgaggc aagcagcccg
     7741 gcggccggcc gggcgcggcg cagttcggtc acgggcaggg cgctcatgcg aggaggcggg
     7801 gcgggcgggg actgcagggt tcgcagccgc cggcgctcgc gtgccggcgg ctgcgctcac
     7861 aggcattccg caggggcgaa gacttgtata taaaccgccg gcccggcggt ttatatacaa
     7921 gtccatgccc tacatcccga cccagaacgc ctggaacggc gaagccgact gccgcacctg
     7981 cagcgtgcgg ggctcggtgc tgttcgccgg cctgcagcag aacgatttcg atctgatcca
     8041 ccagccgatc gacgacatcg ccgtgccggc gcagggccgg gtctatcacg tccatgaacc
     8101 gggcgcgcgc gtgttcacgc tgcgcagcgg cctggtcaag ctcgtgcagt acctgcccga
     8161 cggcagtcag cgcatcgtgc ggctgctgcg cggcaacgac gtgctcgggc tcgaagccct
     8221 gctcggccag ccctaccagc acgatgcgat cgcgctgacc gcggtcgagc tctgcgcgat
     8281 tccgcgcgag gtggtcgcgg tgctggcgcg cgagcagccg gcgctgcaca ccgagctgct
     8341 gaagcgctgg cagcaggcgc tcagcgatgc cgatgtctgg ctgacggagc tttcgaccgg
     8401 cacggcgcgc gcgcgcgtcg cgcgactgct gctgcgtctg gccggcccgc ggccggtcga
     8461 ggcgttccgg ctgctcggac gcgaggacat cggcgcgatg ctcggcctga cgcccgagac
     8521 cgtcagccgc accgtcgccg aactgcgtcg ccagaagctg ctgcgcgagc agggcgccgg
     8581 cctgttcacg gtcgatgcgc cggcgctgcg ccggatcgcc gatggcggct gatgcgcgct
     8641 gccgccgttc ccgtcccgtc gtctgctgtc gtgaggtgga gcaatgaccg gcatccgcgc
     8701 cctgctgttc gacgtgttcg gcacgctggt ggactggcgc acttcggtgg cccgcgaagc
     8761 cgaagccgtg ctggcgccgc tcgggcatgc cctcgactgg ccggcgttcg ccgatgactg
     8821 gcgcgcgcgc taccagccgg ccatggaggc ggtgcgctgc ggtctggagc cctatgttgc
     8881 gctcgatgaa ctgcaccggc gcaacctcga cgccgtgctc gcgagccgcg gcatcgagct
     8941 cgatgaagcc cggtgccggc acctgaacct cgcctggcac cgcctcgacg cctggccgga
     9001 tgtcggcgac gggctcgcgg ccctgcgcgc gccgttcctg ctggcaccgt gctcgaacgg
     9061 caacatcgcg ctgatggtgg cgctggcgcg ccgcaacggc tggcactggg atgcgatc
//
LOCUS       DAOQIE010000148         9115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3856_length_9115_cov_7.561258, whole genome shotgun sequence.
ACCESSION   DAOQIE010000148 DAOQIE010000000
VERSION     DAOQIE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3856_length_9115_cov_7.561258"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK144_10305"
     CDS             complement(<1..208)
                     /locus_tag="PK144_10305"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF66162.1"
                     /translation="MSYTPRNVLVTGGAGFIGANYVRWLLDEDAEVRIVNLDALTYAG
                     SIGNLKGLPGEERHHFVQGDICDAV"
     gene            complement(653..1663)
                     /locus_tag="PK144_10310"
     CDS             complement(653..1663)
                     /locus_tag="PK144_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNF66163.1"
                     /translation="MMKILVTGAAGFIGFHLAQRLLARGDEVIGLDNLNDYYDVALKR
                     ARLAQLEAQPGFRFVGLDLADRPGIAALFEAERFERVVNLAAQAGVRYSLKNPHAYVD
                     SNLVGFVNILEGCRHNGVEHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAASKKAN
                     ELMAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVFNHGRMQRD
                     FTYIDDIVEGVVRVTDRVAAPNPQWSGDAPDAGTSYAPYRLYNIGNNNPVELLRMIEI
                     LEDCLGRRAEKNLLPIQPGDVPATYADVDDLSRDVGFAPATALETGIARFVEWYRAYH
                     GV"
     gene            complement(1704..2825)
                     /gene="wecB"
                     /locus_tag="PK144_10315"
     CDS             complement(1704..2825)
                     /gene="wecB"
                     /locus_tag="PK144_10315"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNF66164.1"
                     /translation="MSAKILCVVGARPNFMKIAPIMAAFRARAPQLVVKLVHTGQHYD
                     VAMNDRFFEQLGIPTPDISLEVGSASHAVQTAEIMKRFEPVLLEERPAAVLVVGDVNS
                     TIACALVAAKLGVRVVHVEAGLRSRDRTMPEEINRILTDQLSDRLYTTEAEALDNLRA
                     EGIDAARVCFAGNVMIDTLRASEAQAVPAAQTLAAAADPAVFRQAGHALLTLHRPSNV
                     DDPVVLARLLGVIDEVSRELPVVFPVHPRTQGKIREAGLGAVLDGTRWLVLPPVGYFE
                     MLGLMREARLVLTDSGGLQEETTALGVPCLTLRENTERPITIAQGTNTLVGTDADAIR
                     AAVREVLATGGKAGRTPERWDGHAAGRIVADLAEWLAPA"
     gene            complement(2905..4182)
                     /gene="tviB"
                     /locus_tag="PK144_10320"
     CDS             complement(2905..4182)
                     /gene="tviB"
                     /locus_tag="PK144_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNF66165.1"
                     /translation="MMDLSNVRIGLIGLGYVGLPLAVEFGKHYPTVGFDIKADRVAEL
                     QAGRDSTLEVEAEELALADQLIYTSALEDIRACNVYIVTVPTPIDAAKRPDLTPLVKA
                     SETLGKVLKRGDVVVYESTVYPGATEEVCVPILEAVSGLKFNLDFFAGYSPERINPGD
                     KEHRVTTIRKVTSGSTPEVAEFVDRLYGSIIVAGTHRASSIKVAEAAKVIENTQRDVN
                     IALINELALIFGRLGIDTEEVLLAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEI
                     GYHPEVILAGRRINDGMGVHVAECVVKLMTQKRMHVVGSRVLVMGLTFKENCPDLRNT
                     RVTDIIAELKTYHAHVDVYDPWVSVAEAEHEYGIHPVSELIDGQYDAIILAVAHNEFR
                     KLGIERIRALGKPGAVLYDIKYVLPRSAVDGRL"
     gene            complement(4238..6070)
                     /gene="glmS"
                     /locus_tag="PK144_10325"
     CDS             complement(4238..6070)
                     /gene="glmS"
                     /locus_tag="PK144_10325"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNF66166.1"
                     /translation="MCGIVGAIAERNVVPILLEGLRRLEYRGYDSAGIATLDDERRLQ
                     RVRAVGKVQALTDSLAANPAPGRLGIAHTRWATHGVPSERNAHPHVSGSELALVHNGI
                     IENYKDLKAELEGLGYRFESETDTEVVAHLVARYLAEERDLLAAVQRAVSRLTGAYSL
                     GVISRHEPGRLIAARQGSPLVIGIGIGEHFIASDVFALLPVTQEFVFLEEGDVADLSR
                     SAFTIYARDGRLVERARATSAQSAGVADKGPYRHYMLKEIHEQPAVVADTLEGRLHGG
                     RVLESCFGHEAGALFDQVRQVHIVACGTSYHAGMVARYWLEAWGVPATVEVASEYRYR
                     DPVIPPGTLFVSISQSGETADTLAALRGAKARGCLASLAICNVPESSLVRESALVLMT
                     RAGREIGVASTKAFTTQLVALRLLAIVLGRRHSLDAAAETQAVAELMQLPARLEQALA
                     LDPVVEHLAETFGAKAHALFLGRGAQFPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALVDPAMPVICVLPDNALHEKVISNLQEVRARGGELFLFADEAVDVHLDGDAVHVLRI
                     APTPDSIAPMVFTVPLQLLAYHVAVLRGTDVDQPRNLAKSVTVE"
     gene            complement(6239..7423)
                     /locus_tag="PK144_10330"
     CDS             complement(6239..7423)
                     /locus_tag="PK144_10330"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNF66167.1"
                     /translation="MSDGILVLNAGSSSIKFAVYADAADGPVEAAHGLIEDIGGAKPH
                     FVAKAPDGAKLHEALPESEPKYDHARALGDLLAWLGAHPELPTPRAVGHRVVHGGTRY
                     SAPVRVDADVRSELERYVPLAPLHQPHNLAGIDAIRALNPDLPQVACFDTAFHRTQPE
                     LARLFALPAQYRNEGVQRYGFHGLSYEYVAGRLPGLIGARGQGKVIVAHLGNGASMCA
                     IEDGRSVASTMGFTAVEGLPMGTRTGSIDPGVLLYLMDAHGMGPKQLTDLLYKRSGLL
                     GLSGVSNDMRTLLASDVPEAKLAVDYFCYRIARELGSLAAALGGLDALVFTGGIGEHA
                     APVRAQVCAQAAWLGIAFDPAANAADAPVITTASSRVTVAVVPTDEEGVIARHARRLT
                     LG"
     gene            complement(7420..8826)
                     /locus_tag="PK144_10335"
     CDS             complement(7420..8826)
                     /locus_tag="PK144_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNF66168.1"
                     /translation="MNDETHLIQNRTFDEIEIGQSASHTRTLNRRDIELFALLSNDLN
                     PTHIDEEFARGAKFSRVVGHSMWSAGLISGVLGTELPGPGTVYRGQTLNFHGVVSVGD
                     VITATVTVSGKNEAARTIELDCRCTDQTGRVVLDGRAEVYAPPVKIHRAAIALPEVHF
                     SEHGARHKKLVDEAARLPAIPTAVVHPVDQVSLEGAVRAAEAGLIVPLLVGPEAKIRA
                     TAEQHGLHIKGFEIVDVPHSHAAAETGVALVREGRAEVLMKGSLHTDEFMGAVVAKAT
                     GLRTGRRVSHAFIMDVPTYPKPLLITDAAINIYPDLMDKADIVQNAIELMHALGVAQP
                     KVAILSAVETVTPKIGSTMDAAALCKMADRGQIKGAILDGPLAFDNAISKSAAKTKGI
                     VSVVPGEADILLAPDLEAGNMIAKQLEYLADAAAAGVVLGARCPIVLTSRADGTLARM
                     ASCAVAALMAHAKRGSLK"
BASE COUNT         1411 a         3035 c         3209 g         1460 t
ORIGIN      
        1 gcacggcgtc gcagatgtca ccctgcacga agtggtgacg ctcctcaccg ggcagaccct
       61 tcaggttgcc gatgctgccg gcgtaggtca gcgcatcgag gttgacgatg cgtacctccg
      121 catcctcatc gagcagccag cgcacgtagt tggcaccgat gaagccggca ccgccggtca
      181 ccagaacgtt gcggggggtg tagctcatgc gttcttccca tctgcagggt ggtccgggcg
      241 gcaaagatca ccggtggcgt accgtcctgc cgtgtacggc ggctcgcccc cgatgtccag
      301 gccacagagc agctcatggc gatacgggcg gcaggggccg gcaccggaga gggccacgaa
      361 aaggctgcgg actgccatga agtcggcacc cgatgattct catgccttgg cccggcgccg
      421 gactccgcca atctgcaggg gagccattgc aatgaccaga agacccggtg cctgcgtccg
      481 gcacctgtat atgagcagcg aaccctgtac cggcccgccg gagaaagacc acggaacagc
      541 gcttgcctct ggtgggcatg cagcgcgccc cgaaccggac tcgaaggcgg cggattattc
      601 cacagcagac aggcacgcag aaggccggga ttgctcggcc agacggcggg gctcagacgc
      661 cgtggtaggc tcgataccat tcgacgaaac gtgcgatgcc ggtctccagc gccgtggccg
      721 gtgcgaagcc gacgtcgcgg ctgagatcat cgacatcggc gtaggtcgcc ggcacgtcgc
      781 cgggctggat tggcagcagg ttcttttcag cgcgccggcc gaggcagtcc tcgaggatct
      841 cgatcatgcg cagcagctcg accgggttgt tgttgccgat gttgtagagc cggtacgggg
      901 cgtagctggt gccggcgtcc ggtgcatccc ccgaccactg cggattgggc gcggcgacgc
      961 gatcggtcac gcgcacgacg ccttcgacga tgtcgtcgat gtacgtgaag tcgcgctgca
     1021 tgcggccatg gttgaacacg tcgatcggcc ggccttcgag gatcgccttc gtgaacagga
     1081 acagcgccat gtccggccgg ccccagggcc cgtagaccgt gaagaagcgc aggccggtgg
     1141 tcggcagccc gtacaggtgg ctgtaggtgt gtgccatcag ctcgttggcc ttcttgctgg
     1201 cggcgtacag gctgaccggg tgatcaacgt tgtgatgaac cgagaacggc atcgcggtgt
     1261 tggcgccata caccgaactg ctcgacgcgt agacgaggtg ttcgacgccg ttgtgccggc
     1321 agccttcgag gatgttgacg aagccgacca ggttgctgtc gacgtaggcg tgcgggttct
     1381 tcagcgaata acggacgccg gcctgcgcgg cgaggttgac tacgcgctcg aagcgttcgg
     1441 cctcgaacaa tgcggcgatg cccgggcggt cagccagatc gagtccgacg aagcggaagc
     1501 ccggctgcgc ttcgagctgc gcgagccgcg cccgcttgag cgccacgtcg tagtaatcgt
     1561 tgaggttgtc gagcccgatg acttcatcgc cgcgcgcgag caggcgctgc gccagatgga
     1621 agccgatgaa gccggcggcg ccggtgacga ggatcttcat gatctgcggt tccgcaaaag
     1681 ggcgatggga ggcagcacgg ccgtcaggcc ggggcgagcc attcggcgag atcggcgacg
     1741 atgcgcccgg cggcatggcc gtcccagcgc tcgggcgtgc gcccggcctt gccgccggtc
     1801 gcgagcacct cacgcaccgc ggcacggatc gcgtcggcgt cggtgccgac cagcgtgttg
     1861 gtgccctgcg cgatcgtgat cgggcgctcg gtgttctcgc gcagcgtcag gcagggcacg
     1921 ccgagcgccg tggtttcctc ctgcaggccg cccgagtcgg tcagcaccag ccgcgcctcg
     1981 cgcatcaggc cgagcatctc gaagtagccg accggcggca gcaccagcca gcgcgtgccg
     2041 tcgagcacgg caccgaggcc ggcctcgcga atcttgccct gcgtgcgcgg atgcaccgga
     2101 aacacgaccg gcagctcgcg gctcacctcg tcgatcacgc cgagcaggcg cgcgagcacg
     2161 accggatcat cgacgttcga tggccggtgc agcgtcagca gcgcatggcc agcctgccgg
     2221 aacacggccg gatccgcggc cgcagccagc gtctgcgcgg ccggtacggc ttgcgcctcg
     2281 ctggcgcgca gcgtgtcgat catgacgttg ccggcgaagc agacgcgcgc ggcatcgatg
     2341 ccttcggccc tgaggttgtc gagcgcctcg gcctcggtcg tgtacaggcg gtccgagagc
     2401 tgatcggtca ggatgcggtt gatctcctcg ggcatcgtgc ggtcgcgcga gcgcagcccg
     2461 gcctcgacgt gcacgaccct gacgccgagc ttcgcggcga ccagcgcgca ggcgatcgtc
     2521 gagttgacat ccccgaccac cagcaccgcc gccggccgtt cctcaagcag taccggctcg
     2581 aagcgcttca tgatttcggc ggtctgcacc gcgtgcgagg ccgagccgac ctcgagcgag
     2641 atgtccggcg tcgggatgcc gagctgctcg aagaagcggt cattcatcgc cacgtcgtag
     2701 tgctggccgg tgtgcacgag cttgacgacc agttgcgggg cccgggcacg gaacgcggcc
     2761 atgatcgggg cgatcttcat gaagttcggc cgggcgccga cgacgcagag aatcttcgct
     2821 gacatggact taccggagct gacggatgcg aaaccgcccg cttcgggggc gggcggcgtt
     2881 tcggtggcag gcgaaggccg aagctcagag ccggccgtcg acggcgctgc gcggcagcac
     2941 gtacttgatg tcgtagagca cggcgccggg cttgccgagg gcgcggatgc gctcgatgcc
     3001 gagcttgcgg aactcgttgt gcgcgacggc gaggatgatc gcgtcgtact ggccgtcgat
     3061 cagctccgag accggatgga tgccgtactc gtgttcggcc tcggccaccg agacccatgg
     3121 atcgtagaca tcgacgtgcg cgtggtaggt cttcagctcg gcgatgatgt cggtgacgcg
     3181 ggtgttgcgc aggtccgggc agttttcctt gaaggtcagg cccatgacca gcacgcgcga
     3241 gccgaccacg tgcatgcgct tctgcgtcat cagcttgacc acgcattcgg ccacgtgcac
     3301 gcccatgccg tcgttgatgc gccggccggc gaggatgacc tcggggtgat agccgatctc
     3361 ctgcgccttg tgcgtcaggt agtacggatc gacgccgatg cagtgcccgc cgaccagccc
     3421 gggccggaac ggcaggaagt tccacttggt gccggcggcg agcaggactt cctcggtgtc
     3481 gatgccgaga cgaccgaaga tcagcgccag ctcattgatc agcgcgatgt tcacgtcgcg
     3541 ctgcgtgttc tcgatgacct tggcggcctc ggcgaccttg atgctgctcg cgcggtgcgt
     3601 gccggcgacg atgatgctgc cgtacagccg gtcgacgaac tcggcgacct cgggcgtcga
     3661 gccggaggtg accttcctga tggtcgtgac gcggtgttcc ttgtcgccgg ggttgatgcg
     3721 ctcggggctg tagccggcga agaagtcgag gttgaacttc aggcccgaca ccgcttcgag
     3781 gatcggcacg cagacctcct cggtcgcgcc cggatacacc gtcgattcat aaacgaccac
     3841 gtcgccgcgc ttgagcacct tgccgagcgt ctcgctggcc ttcacgagcg gcgtgagatc
     3901 cggacgcttg gcggcgtcga tcggcgtcgg caccgtgacg atgtagacat tgcaggcacg
     3961 gatgtcttcg agcgcgctcg tgtagatcag ttgatcggca agcgcgagtt cctcggcttc
     4021 gacttcgagc gtgctgtcgc ggccggcctg cagctcggcg acgcgatcgg ccttgatgtc
     4081 gaaaccgacg gtcggatagt gcttgccgaa ctcgacggcc agcggcaggc cgacgtagcc
     4141 gaggccgatc aggccgatgc ggacgttgct gagatccatc attggtctct ctggagggga
     4201 aagggggagg aattccggcc tggcgggcgg cggcgcccta ctccaccgtc accgatttcg
     4261 cgaggttgcg cggctggtcg acatcggtgc cgcgcagcac tgcgacgtgg taggccagca
     4321 gctgcagcgg caccgtgaac accatcgggg cgatcgaatc cggggtcggt gcgatacgca
     4381 gcacgtgcac ggcgtcgcca tccagatgca catcgaccgc ctcgtcggcg aacaggaaca
     4441 gctcgccgcc acgggcgcgc acttcctgca ggttcgagat caccttctcg tgcagcgcgt
     4501 tgtccggcag cacgcagatc accggcatcg ccggatcgac cagcgccagc ggaccgtgct
     4561 tgagttcgcc ggccgcgtag gcctctgcat ggatgtagct gatttctttg agcttcagcg
     4621 ccccttcaag ggcgatcggg aactgcgcgc cgcgcccgag gaacagcgcg tgcgccttgg
     4681 ccccgaaggt ctcggccaga tgctcgacga ccggatcgag cgccagcgcc tgctccaggc
     4741 gtgccggcag ctgcatgagt tcggcgacgg cctgtgtctc ggctgccgcg tcgaggctgt
     4801 ggcggcgacc gagcacgatc gcgagcaggc gcagcgccac cagctgggtc gtgaacgcct
     4861 tggtcgaggc cacaccgatc tcgcggccgg cgcgggtcat cagcaccagc gccgattcgc
     4921 gcaccagcga gctttccggc acgttgcaga tcgcgaggct cgccagacac ccgcgtgcct
     4981 tcgcaccgcg cagcgccgca agcgtgtcgg cggtctcgcc ggactgcgag atgctgacga
     5041 acagcgtgcc gggcgggatc accgggtcgc ggtagcggta ttcgctcgcg acctcgaccg
     5101 tggccggcac gccccaggct tcaagccagt accgcgcgac cataccggcg tgatagctgg
     5161 tgccgcaggc gacgatgtgg acctggcgca cctgatcgaa cagcgcgccg gcctcgtggc
     5221 cgaagcagct ttccagcacg cggccgccgt gcagccggcc ttcgagcgtg tcggcgacca
     5281 cagccggctg ctcgtggatt tccttgagca tgtagtgccg gtacggcccc ttgtcggcga
     5341 caccggcact ctgcgccgag gtcgcgcgcg cacgctcgac cagccggccg tcgcgcgcgt
     5401 agatcgtgaa cgcgctgcgc gacaggtcgg caacatcgcc ttcctccagg aacacgaact
     5461 cctgcgtgac cggcagcagc gcgaacacgt cggacgcgat gaagtgctcg ccgatgccga
     5521 tgccgatcac cagcggcgag ccctgacgtg cggcgatcag ccggcccggc tcgtggcgcg
     5581 agatcacgcc gaggctgtag gcgccggtca ggcgcgacac cgcgcgctgc accgcggcga
     5641 gcaggtcgcg ctcctcggcc aggtagcgcg cgaccagatg cgcgacgacc tcggtgtcgg
     5701 tctcggattc gaagcgatag ccgagtcctt cgagttcggc cttcagatcc ttgtagttct
     5761 cgatgatgcc gttgtgcacc agcgcgagct cgctgccgct gacgtgcgga tgagcgttgc
     5821 gctcgctcgg cacgccgtgc gtggcccagc gcgtgtgtgc gatgccgagc cggcccggag
     5881 cgggattcgc cgccaggctg tcggtgagcg cctggacctt gccgaccgcg cgcacgcgct
     5941 ggagacgacg ctcgtcatcc agggtcgcaa tcccggcgga gtcgtagccg cggtattcga
     6001 gccggcgcag tccctccagc aggatcggga cgacgttgcg ctcggcgatg gcgccgacga
     6061 ttccgcacat ggcaaggatt cctgttgcag cgggaggata cggggcgcca gcgtaccgac
     6121 ccgcgccgcc gaccgacaag ccggccggcg ggacggtcga tgattgttca cgtggataaa
     6181 cgggaagtcc ggccatgagg cgtgagcagg gcaccgcccg ctcacgcccc gggcttcctc
     6241 accccagcgt cagccggcgt gcgtgccggg cgatgacgcc ttcctcgtcg gtcggcacga
     6301 ccgcgaccgt cacgcggctc gatgccgtcg tgatcaccgg cgcatccgcg gcgttggccg
     6361 ccggatcgaa ggcgatgccg agccaggccg cctgcgcgca gacctgcgcg cgcaccggcg
     6421 cggcgtgctc gccgatgccg ccggtgaaca ccagcgcatc gagtccgccg agcgcggctg
     6481 cgagtgaacc gagttcgcgc gcgatgcggt agcagaagta atcgaccgca agcttcgcct
     6541 ccggcacgtc cgaggcgagc agcgtgcgca tgtcgttgga cacgccggac agcccgagca
     6601 ggcccgagcg cttgtacagc aggtcggtga gctgcttcgg ccccatgccg tgcgcgtcca
     6661 tcaggtacag cagcacgccg gggtcgatcg agccggtgcg cgtgcccatc ggcaggccct
     6721 cgacggccgt gaagcccatc gtgctcgcga cgcttctgcc gtcctcgatc gcgcacatcg
     6781 aggcaccgtt gcccagatgc gcgacgatga ccttgccctg cccgcgcgcg ccgatcagcc
     6841 cgggcaggcg cccggcgacg tattcatacg acaggccgtg gaagccgtag cgctgcacgc
     6901 cctcgttgcg gtactgcgcc ggcagcgcga acagccgtgc cagctccggc tgcgtgcggt
     6961 ggaaggcggt gtcaaagcac gccacctgcg gcagatccgg attcagcgcg cggatcgcgt
     7021 cgatgccggc gaggttgtgc ggctggtgca gcggggcgag cggcacgtag cgctccagtt
     7081 cgctgcggac gtcggcatcg acgcgcaccg gggcgctgta gcgcgtgccg ccgtgcacga
     7141 cccggtggcc gacggcgcgc ggcgtcggca gttccggatg cgcgccgagc caggcgagca
     7201 ggtcgccgag cgcgcgggca tggtcgtact tcggctcgga ctccggcagc gcctcgtgca
     7261 gcttcgcacc atccggcgcc ttggcgacga agtgcggctt ggcaccgccg atgtcctcga
     7321 tcaggccgtg cgcggcctcg accggcccgt ctgcggcatc ggcatagact gcgaacttga
     7381 tgctcgacga accggcgttc aggaccagga tgccgtcact catttcagcg acccccgctt
     7441 ggcgtgcgcc atcagcgccg cgaccgcaca cgaggccatg cgcgccagcg tgccgtcggc
     7501 gcgcgaggtc agcacgatcg ggcagcgcgc gccgagcacg acgccggccg ccgcggcatc
     7561 ggcgaggtat tcgagctgct tggcgatcat gttgccggct tcgagatcgg gcgcgagcag
     7621 gatgtcggcc tcgccgggca cgaccgagac gatgcccttg gtcttggccg cggacttgct
     7681 gatggcgttg tcgaaggcaa gcggcccgtc gaggatcgcg cccttgatct ggccgcggtc
     7741 ggccatcttg cagagcgccg cggcatccat ggtcgaaccg atcttcggcg tcaccgtctc
     7801 gaccgccgac aggatcgcga ccttcggctg cgccacgccg agcgcatgca tcagctcgat
     7861 ggcgttctgc acgatgtcgg ccttgtccat caggtccgga tagatgttga tcgccgcgtc
     7921 ggtgatcagc agcggcttcg gataggtcgg cacgtccatg atgaaggcat ggctgacgcg
     7981 ccggccggtg cgcaggcccg tggccttggc caccaccgcg cccatgaatt cgtcggtgtg
     8041 cagactgccc ttcatcagca cctcggcgcg gccctcgcgc accagcgcca cgccggtctc
     8101 ggcggccgcg tgcgaatgcg gcacgtcgac gatctcgaaa cccttgatgt gcaggccgtg
     8161 ctgctcggcg gtggcgcgga tcttggcctc gggtccgacc agcagcggca cgatcagccc
     8221 ggcctcggcg gcgcgcaccg cgccttcgag cgagacctga tcgaccgggt gcacgaccgc
     8281 ggtcgggatc gccggcaggc gcgcggcctc gtcgacgagc ttcttgtggc gcgcgccatg
     8341 ctcgctgaaa tgcacctcgg gcagcgcgat cgcggcgcgg tggatcttga ccggcggcgc
     8401 gtagacctcg gcgcgaccgt cgagcaccac gcgaccggtc tggtcggtgc agcggcagtc
     8461 gagctcgatc gtgcgcgccg cttcgttctt gccgctgacc gtgaccgtgg cggtgatcac
     8521 gtcaccgacc gacacgacgc cgtggaaatt cagcgtctgg ccgcgataga ccgtgcccgg
     8581 ccccggcagc tcggtgccga gcacgccgga gatcaggccg gcgctccaca tgctgtggcc
     8641 gaccacgcgc gagaacttgg cgccgcgcgc gaattcctcg tcgatgtgcg tgggattcag
     8701 atcgttcgac agcagcgcga acagctcgat gtcgcggcgg ttcagcgtgc gcgtgtggct
     8761 ggcactctgg ccgatctcga tctcgtcgaa ggtgcggttc tggatcagat gggtttcgtc
     8821 gttcatgacc gtcgttccgg gacaagcgtg aggggaaagg tgcgaaggtg gggtgaacca
     8881 gggcgacgtg gcaggcaggg cgtgaattca cgccctgccc ccgcgcctca ccgcacgcag
     8941 cctcagcccc ggacgtggta gcccgcgtcg acgaaaccga tgttgccggt gatgttgttg
     9001 ccggtctggg cgatgtagaa ggccgcgagc cggccgacgt cctcgggcgt cgccagccgt
     9061 ccgagcggcg agcgcgtggt cgcatcggcc atcagctcgt cgaagtcctt gatgc
//
LOCUS       DAOQIE010000149         9072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3883_length_9072_cov_6.965399, whole genome shotgun sequence.
ACCESSION   DAOQIE010000149 DAOQIE010000000
VERSION     DAOQIE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_9072_cov_6.965399"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PK144_10340"
     CDS             complement(<1..628)
                     /locus_tag="PK144_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNF66169.1"
                     /translation="MASNPKEDAGRTPAAARAPRSRGLLGTVLLGLVLLVAVLALNPV
                     YTVESGEVVVLSTFGRYDPEPRLPGLHFRMPLVQSTHFLDVRLQTLNFRGREDAPDGD
                     GILNHPGIQVLDAKNLPITLDLSLQFTPHAAEAAGLLERYGRNYADRLIVPLVREVVR
                     DVVGGYQSEQIANQRSEIGRGLEAKLIEALKAYPFTVENVALREIGLPA"
     gene            709..1092
                     /locus_tag="PK144_10345"
     CDS             709..1092
                     /locus_tag="PK144_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNF66170.1"
                     /translation="MNLEKVVFGFFILLAATLNFGFVLGEIDNPVHHDVYELAAALVV
                     SLIATVLKLGDRTHMGAVLLATSLVADLQLIAAAVVWAIAAHAYGAGEAPRVVSSVVS
                     LASGAALANFISIVMLLAETINLRR"
     gene            1105..2922
                     /locus_tag="PK144_10350"
     CDS             1105..2922
                     /locus_tag="PK144_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF66171.1"
                     /translation="MNLVHLLLQRRRPGSGGDTAPLDPREGSAAAINSVVFLFLRRMR
                     LPLVVLISVYAVSIAGLVAIPGVDPEGRPWRFDLFHAFYFISYTGSTIGFGEVPYAFT
                     PAQRLWVTVCIYLTVIGWLYAVGTILNLLQDPAFRRALAESRFAGEVRGLAEPFWIIC
                     GYGEAGSLLVRALCRRGVQTVVLDADAERLARLDLEDVGFDVPCLAADARASRILRLA
                     GLVHPRCLGVVAIAADDAIDVKVAITAKLLNPRLRVIARAEHASTAANLASFETDRII
                     DAYETFGEHLAMALVEPSLHMLYEYLIRQPGQPLPPRLVPPRGQWIVCGHGRFGSAAY
                     RHLAGAGLPVRVIESDPDDAPEDAVLGDATTANTLCSAGALSAAGLVAGTDDDADNLS
                     IAYTARLLNPDLFVIARANHREEEPLFAAAGLDLVISPSRLIVWRVLGELIAPQTNRF
                     LALAQRRGEAWAAPLLARLDACSEGLTPDTWETTIDAAQAPALHALLAAGRRPDFAAL
                     LRDPQDRSRPLPVLLLLRVGADGHEQLLPPPEAQPVAGDRLLFAGRSGSAATLAWIFG
                     SPKTLEYVLDGVEHPDGTVWRWLARRRAAHPRPVQGNTP"
     gene            3230..4432
                     /locus_tag="PK144_10355"
     CDS             3230..4432
                     /locus_tag="PK144_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HNF66172.1"
                     /translation="MSAAQPALADRLHQRLFDALYAHSLVYNACWEDPAVDRRALAIG
                     PDDRLLVITSAGCNVLDYALCAPAAIHAVDANPRQSALLELKLAGIRRLAYADFWALF
                     GEGAHRYAPELYRDTLRAELSPFAARYWDARIDWFAPRQPQQGFYWRGLSGWVARGFR
                     ALLVARPKLRHAVEALLEAHDLDAQRAHYDAAVAPLLWNRSVRWAVSRPLTMSLLGVP
                     HPQRIEVERQHPDGIAGFVREAIDYVFRELPLADNYFWALYLRGRYTPDCCPEYLKRD
                     NFYRLKDGLAARIAVHTDTVTGFLQATDERLSRFVLLDHMDWMSAFHPQALAAEWAAI
                     LDRATPDARLIFRSAHARPEYLETIDVPGRGPLTARLHFERELAGRLHAGDRVHTYAG
                     FHIATLAA"
     gene            4562..5296
                     /locus_tag="PK144_10360"
     CDS             4562..5296
                     /locus_tag="PK144_10360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF66173.1"
                     /translation="MSIAGDARVLAALLRGQPRDGDHAARLDAFYAPQAAAYDGFRER
                     LLHGRRELMLELAARVAPGGTLVELGGGTGRNLGFLGAQLADFDAVTLVDLCRPLLAQ
                     ARQRAAHWPNVRIVEADACDWQPSAPVDAVVCAYALSMIPDWYRAIDNAVAMLAPGGL
                     LAVADFYVSRARPAPGHVRHSTFARHFWPLWFGHDGVHPSPDHLPCLESKLDTVLLRE
                     ARAPLPWLPGLRAPYYVWIGRRRGAG"
     gene            5380..6174
                     /locus_tag="PK144_10365"
     CDS             5380..6174
                     /locus_tag="PK144_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF66174.1"
                     /translation="MSHSPYLAAAIEAARAGEEVIRKYYQSNLAITVKADRTPVTVAD
                     VETEQAIKGVLTTAFPEHGFFGEETGRSNADSEYLWLVDPIDGTKSFVREYPFFSTQI
                     ALMHRGELVLGVSNAPVFGDGEMAYAERGYGSFLNGSRIRVSDFDALDAATLSLGNIK
                     TLTKDESCWTRIGELIRSVSRIRGFGDFYHYHLLASGKIELIVESDVNILDVAALSVI
                     IGEAGGTVTQFDGRPLDLDATTIVASNGRLHGAALDAIGGCRACVS"
     gene            6162..6680
                     /gene="cobU"
                     /locus_tag="PK144_10370"
     CDS             6162..6680
                     /gene="cobU"
                     /locus_tag="PK144_10370"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNF66175.1"
                     /translation="MRELILGGARSGKSRYAAERARRSGLDVRCIVTAEAFDAEMQER
                     ITRHRAERPAHWRTIESPRGLAETLAAQAAPTRCLLVDCLTLWLSNLLCAGDEAALEA
                     ETEALLALAPTLPGRLLLVSNEVGWGIVPDDPLARRFRDEAGRLHQRLAGRLERVTLV
                     VAGLPLALKTPS"
     gene            6677..7729
                     /gene="cobT"
                     /locus_tag="PK144_10375"
     CDS             6677..7729
                     /gene="cobT"
                     /locus_tag="PK144_10375"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNF66176.1"
                     /translation="MSEPFFDWLHAPLRRPDAAAAAEAAARQAQLTKPAGSLGRLEAL
                     AIRLAGLQGTPRPRLDRVHIAVFAADHGVVAEGVSAYPQAVTAEMLRNFAHGGAAIAV
                     LARLHAATLEVVDLGTVVPPGPLPGVLDRRIAPGTRNLAREPAMTDEQLGRALAAGAE
                     SVARAHAAGAQLYIGGEMGIGNTTASAALAAALLDQPAAALVGPGTGVDADGLRRKLA
                     AVEAALARARTSDPLAALREFGGFELAALTGAYLAAAQHGLPVLVDGFIASVAALAGL
                     RLQPALADWLLYAHCSAEPGHRRVLAALGAEPLLDLGLRLGEASGAAVALPLLRMACA
                     LHDEMATFADAGVSRA"
     gene            7726..8349
                     /locus_tag="PK144_10380"
     CDS             7726..8349
                     /locus_tag="PK144_10380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014420183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF66177.1"
                     /translation="MNRYTTVIDLMRHGEPEGGRLFRGSKDDPLSATGWQQMRTVAAE
                     FRPWQAVVSSPLRRCREFAEELATRHGLPVEIDPLFREIGFGDWEGLSAEQVEVRHPG
                     ALAEYWRAPEGYTPPGGEPLVRFDARIEAAWDGLLERHRGAHVLLVCHGGVIRACLRQ
                     VLGLPLANLWRIDVPYAAPTRVRIHGDHGEPVPILEFLNGGFAGWWL"
     gene            complement(8363..8770)
                     /locus_tag="PK144_10385"
     CDS             complement(8363..8770)
                     /locus_tag="PK144_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HNF66178.1"
                     /translation="MSRRTGACDPACPCGSGRAFADCCSPYLAGSAEPPTAEALMRSR
                     YSAFVRRDERWLRTSWHPDTCPAGLDVAADPVQWLGLKVLAVQGGGAGDAEGTVEFVA
                     RGKRNGRAFRMHETSRFVRVDGAWRYLDGDISN"
     gene            complement(8864..>9072)
                     /locus_tag="PK144_10390"
     CDS             complement(8864..>9072)
                     /locus_tag="PK144_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005303151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNF66179.1"
                     /translation="RPNFGSRNGPTELEAGTALTAEADALRALGHELRILELNSGVQA
                     IRRAAGGGWIGAADARREGRAGGE"
BASE COUNT         1167 a         3391 c         3171 g         1343 t
ORIGIN      
        1 cggccggcag cccgatctcg cgcagcgcga cgttctcgac cgtgaacgga taagccttca
       61 gcgcctcgat gagcttcgct tcgagcccgc ggccgatctc gctgcgctgg ttggcgatct
      121 gctcgctctg gtagccgccg accacgtcgc gcacgacctc gcgcaccagc ggcacgatca
      181 gccggtcggc gtagttgcgg ccatagcgtt cgagcaggcc ggcggcctcg gcggcatgcg
      241 gcgtgaactg cagcgacaga tcgagcgtga tcggcaggtt cttggcgtcg agcacctgga
      301 tgccggggtg attgaggatg ccgtcgccgt ccggcgcatc ctcgcggccg cggaagttca
      361 gcgtctgcag gcgcacgtcg aggaagtgcg tgctctgcac cagcggcatg cggaagtgca
      421 ggccgggcag gcgcggttcg gggtcgtagc ggccgaaggt cgacagcacg acgacctcgc
      481 cgctctcgac cgtgtacacc gggttcagcg ccagcaccgc caccagcagc accagcccca
      541 gcagcacggt gccgagcagg ccgcgcgagc ggggcgcgcg ggcagcggcc ggggtgcggc
      601 cggcgtcctc tttcgggttc gaagccatgt tccctcccgg gctttcacga acggcggcgg
      661 ccggctgcct acactcggcc gacaacgaca ccgccagggg gatggtgcat gaatctcgaa
      721 aaggtcgtct tcggcttctt catcctgctc gccgcgacgc tgaacttcgg cttcgtgctc
      781 ggcgagatcg acaatcccgt gcatcacgac gtctacgagc tggccgcggc gctggtcgtg
      841 agcctgatcg cgacggtgct gaagctcggg gatcgcacgc acatgggcgc ggtgctgctc
      901 gcgaccagtc tggtcgcgga cctgcagctg atcgcggcgg ccgtggtctg ggcgatcgcg
      961 gcgcacgcct acggggccgg cgaggcaccg cgggtcgtgt cctcggtggt gtcgctcgcc
     1021 agcggcgcgg cgctcgcgaa cttcatctcg atcgtcatgc tgctcgccga aaccatcaac
     1081 ctgcgccgct gacgaccgcc gaagatgaac ctcgtccacc tgctgctgca gcgtcgccgg
     1141 cccggctccg ggggcgacac ggcgccgctc gatccgcgcg aaggcagcgc ggcggcgatc
     1201 aacagcgtcg tgttcctgtt cctgcggcgc atgcgcctgc cgctggtggt gctgatcagc
     1261 gtctacgcgg tgtcgatcgc cgggctggtc gcgattccgg gtgtcgatcc ggagggccgg
     1321 ccgtggcgct tcgacctgtt ccacgccttc tacttcatca gctacaccgg ttcgacgatc
     1381 ggcttcggcg aggtgccgta cgcgttcacg ccggcgcagc ggctgtgggt gacggtgtgc
     1441 atctacctga ccgtgatcgg ctggctgtat gcggtcggca cgatcctgaa cctgctgcag
     1501 gacccggcct tccgccgggc gctggccgag agccgcttcg ccggcgaggt gcgcgggctg
     1561 gccgagccgt tctggatcat ctgcggctac ggcgaggccg gcagcctgct ggtgcgggcg
     1621 ctgtgccggc gcggcgtgca gacggtcgtg ctcgatgccg atgccgagcg cctcgcgcgg
     1681 ctcgacctgg aggacgtggg cttcgacgtg ccgtgcctcg cggccgatgc gcgcgcctcg
     1741 cgcatcctgc ggctggccgg gctcgtgcat ccgcgctgcc tcggcgtggt cgcgatcgct
     1801 gccgacgatg cgatcgacgt gaaggtcgcg atcaccgcga agctgctgaa cccgcgcctg
     1861 cgcgtgatcg cgcgcgccga gcacgcctcg accgcggcca atctcgcgtc gttcgagacc
     1921 gaccgcatca tcgatgccta cgagaccttc ggcgagcatc tggccatggc gctggtcgag
     1981 ccgagcctgc acatgctcta cgaatacctg atccgccagc ccggccagcc gctgccgccg
     2041 cggctggtgc cgccgcgcgg gcagtggatc gtctgcggtc acgggcgctt cggcagcgcc
     2101 gcgtaccggc atctggccgg cgccggcctg ccggtgcgcg tgatcgagtc cgatccggac
     2161 gatgcgcccg aggatgccgt gctcggcgat gcgacgaccg ccaacacgct gtgctcggcc
     2221 ggggcgctca gtgcggccgg gctggtcgcc ggcaccgacg acgacgccga caacctgtcg
     2281 atcgcctaca cggcgcggct gctgaatccg gacctgttcg tgatcgcacg cgccaatcac
     2341 cgcgaggagg agccgctgtt cgcggccgcc ggcctcgatc tggtgatctc gccgagccgg
     2401 ctgatcgtct ggcgcgtgct cggcgagctg atcgcgccgc agaccaaccg cttcctcgcg
     2461 ctcgcgcagc gccgcggcga ggcctgggcc gcgccgctgc tggcacggct cgacgcctgc
     2521 agcgagggcc tgacgcccga tacctgggag acgacgatcg acgccgcgca ggccccggcc
     2581 ctgcacgcgc tgctcgccgc cgggcggcgg ccggacttcg cggcgctgct gcgcgacccg
     2641 caggaccgca gccggccgct gccggtgctg ctgctgctgc gcgtcggcgc cgatggccac
     2701 gaacagctgc tgccgccgcc cgaggcgcag ccggtcgccg gcgaccggct gctgttcgcc
     2761 ggccggagcg gcagcgcggc gacgctcgcg tggatcttcg gcagcccgaa gacgctcgaa
     2821 tacgtgctcg atggcgtcga gcaccccgac ggcacggtgt ggcgctggct cgcacgccgg
     2881 cgggccgcgc atccgcggcc ggtgcaggga aacactcctt aacgtgcccc taagtcggcg
     2941 gcgactaggc ttgcctccat cgagccggac ggcaagccgg agcgatatct cccaggtcac
     3001 gcagcatgct gggggcgacc ggcagacccg gtcgccccct ctttttttgc ctgcgccttc
     3061 tgcctgcgcc ttccgcctgc cctgcctgcc tcgtgcccgc cggttttccg cagcgagccg
     3121 cggcgcgcac atcccgcgcg tcaccgttcc gaccgcggcc cgtcaccgga cggtcatcgc
     3181 cgccaagcag gctgcggcga atgccccgag ccttccggag aacccgacgg tgtccgccgc
     3241 ccagcccgct cttgccgacc gcctgcacca gcgtctgttc gatgcgctct acgcgcactc
     3301 gctggtctac aacgcctgct gggaagaccc ggccgttgat cgccgggcgc tggcgatcgg
     3361 ccccgacgac cggctgctgg tgatcaccag tgccggctgc aacgtgctcg actacgcgct
     3421 gtgcgccccg gccgcgatcc acgccgtcga cgccaatccg cgccagagcg cgctgctgga
     3481 gctgaagctc gccggcatcc ggcggctggc ctatgccgac ttctgggcgc tgttcggcga
     3541 aggcgcgcac cgctacgcgc ccgagctgta ccgcgacacg ctgcgcgccg agctgtcgcc
     3601 gttcgcggcg cgctactggg atgcgcgcat cgactggttc gcgccgcgcc agccgcagca
     3661 gggcttctac tggcgcgggc tgtcgggctg ggtcgcgcgc ggtttccggg cgctgctggt
     3721 ggcgcgcccg aagcttcgcc acgccgtcga ggcgctgctc gaagcgcacg atctcgatgc
     3781 ccagcgcgcg cactacgacg ccgcggtcgc accgctgctg tggaaccgca gcgtgcgctg
     3841 ggcggtgtcg cggccgctga cgatgagcct gctcggcgtg ccgcacccgc agcgcatcga
     3901 ggtcgagcgc cagcatccgg acggcatcgc cggcttcgtg cgcgaggcga tcgactacgt
     3961 gttccgcgag ctgccgctcg ccgacaacta cttctgggcg ctgtacctgc gcggccgcta
     4021 cacgccggac tgctgcccgg agtacctgaa gcgcgacaac ttctaccggc tgaaggacgg
     4081 gctcgccgcg cgcatcgccg tgcacaccga caccgtcacc ggcttcctgc aggccaccga
     4141 cgagcgcctg tcgcgcttcg tgctgctcga tcacatggac tggatgagcg ccttccatcc
     4201 gcaggcgctc gccgccgaat gggcagcgat ccttgatcgg gcgacgccgg atgcgcgact
     4261 gatcttccgc agcgcccacg cccggcccga gtacctggaa acgatcgacg tgcccggccg
     4321 cggcccgctg acggcccggc tgcacttcga gcgcgagctg gccgggcgcc tgcacgccgg
     4381 cgaccgcgtg cacacctacg cgggcttcca catcgccacg ctcgccgcct gaccgacctc
     4441 tgcgcgaatg cgcggttttt ccctctctcc gccctccccc gcgagggcgc aggggccgaa
     4501 aaaacgcgcg ttccgcagtg ctcggcctgc atgtccctga acgccgcccg gaggaagccc
     4561 gatgtcgatc gccggagatg cccgcgtcct cgccgcgctg ctgcgcggcc agccccgtga
     4621 cggcgaccac gccgcgcggc tcgacgcctt ctacgccccg caggccgctg cctacgacgg
     4681 cttccgcgag cgcctgctgc acggccggcg cgagctgatg ctcgaactcg ccgcgcgcgt
     4741 cgcgcccggc ggcacgctgg tcgaactcgg cggcggcacc ggccgcaacc tcggcttcct
     4801 cggcgcgcag ctcgccgatt tcgacgcggt gacgctggtc gatctgtgcc ggccgctgct
     4861 cgcgcaggcg cgccagcgcg ccgcacactg gccgaacgtg cgcatcgtcg aggccgacgc
     4921 ctgcgactgg cagccttcgg cgccggtcga tgccgtggtc tgcgcctatg cgctgtcgat
     4981 gatcccggac tggtatcgcg ccatcgacaa cgccgtcgcg atgctcgcgc ccggcggcct
     5041 gctcgcggtc gccgacttct acgtctcgcg cgcgcggccg gcgcccggtc acgtgcgcca
     5101 ctcgaccttc gcgcggcact tctggccgct gtggttcggg cacgacggcg tgcatccgag
     5161 cccggatcac ctgccctgcc tcgaatcgaa gctcgacacc gtgctgctgc gcgaggcgcg
     5221 cgcgccgctg ccgtggctgc cgggcctgcg cgcgccgtat tacgtgtgga tcggccgccg
     5281 gcgcggtgcc ggctgagcgc cacgcaccgg gcatgggccg ctcgcggagc gctccgttag
     5341 actgcagcgt ggaattcccc ctgcagagac cccgtaccga tgagccacag cccctatctc
     5401 gccgccgcca tcgaggccgc gcgtgccggc gaggaagtca tccgcaagta ctaccagagc
     5461 aacctcgcga tcacggtcaa ggccgaccgc acgccggtca cggtcgccga cgtcgagacc
     5521 gagcaggcga tcaagggcgt gctgacgacg gccttcccgg agcacggctt cttcggcgag
     5581 gaaaccgggc gcagcaacgc cgattccgag tatctgtggc tggtcgaccc gatcgacggc
     5641 accaagagct tcgtgcgcga gtacccgttc ttctcgacgc agatcgcgct gatgcaccgc
     5701 ggcgagctgg tgctcggggt ttccaacgca ccggtgttcg gcgatggcga gatggcctac
     5761 gccgagcgcg gctacggcag cttcctgaac ggctcgcgca tccgcgtcag cgacttcgac
     5821 gcgctcgatg ccgcgacgct gtcgctcggc aacatcaaga cgctgacgaa ggatgaaagc
     5881 tgctggacgc gcatcggcga gctgatccgc agcgtctcgc gcatccgcgg cttcggcgac
     5941 ttctaccact accacctgct cgcctccggc aagatcgagc tgatcgtcga gtccgacgtg
     6001 aacatcctcg atgtcgcggc gctgtcggtg atcatcggcg aggccggcgg cacggtgacg
     6061 cagttcgacg gccggccgct cgatctcgac gcgacgacca tcgtcgccag caacggcagg
     6121 ctgcacggtg ccgcactcga tgcgatcggc gggtgccgcg catgcgtgag ctgatcctcg
     6181 gcggcgcccg ctccggcaag tcgcgctacg ccgccgaacg cgcccgccgc tcgggcctcg
     6241 acgtgcgctg catcgtcacc gccgaggcat tcgatgccga gatgcaggag cgcattaccc
     6301 gccaccgcgc cgagcggccc gcgcactggc gcacgatcga gtcgccgcgc ggcctcgccg
     6361 aaaccctggc cgcccaggcc gcgccgaccc gctgcctgct ggtcgactgc ctgacgctgt
     6421 ggctgtcgaa cctgctctgc gccggtgacg aggccgcgct cgaagccgag accgaagcgc
     6481 tgctcgcgct ggccccgacg ctgccgggcc ggctgctgct ggtctcgaac gaggtcggct
     6541 ggggcatcgt gccggatgat ccgctggcgc ggcgcttccg cgacgaggcc ggccgcctgc
     6601 accagcgcct cgccgggcgg ctggagcgcg tgacgctggt cgtcgccggc ctgccgctgg
     6661 cgctgaagac gccgtcatga gcgaaccctt cttcgactgg ctgcacgcac cgctgcgcag
     6721 gccggatgcc gccgctgccg ccgaagcggc ggcgcgtcag gcccagctca ccaagccggc
     6781 cggcagcctc ggccgcctcg aagcgctggc gatccggctc gccggcctgc agggcacgcc
     6841 gcgcccgcgg ctcgatcgcg tgcacatcgc ggtgttcgcg gccgatcacg gcgtcgtcgc
     6901 cgagggcgtg tcggcctatc cgcaggcggt cacggccgag atgctgcgca acttcgcgca
     6961 cggcggcgcg gcgatcgcgg tgctcgcgcg cctgcacgcc gcgacgctcg aagtcgtcga
     7021 tctcggcacc gtcgtcccgc ccggtccgct gcccggcgtg ctcgaccggc gcatcgcccc
     7081 cggcacgcgc aacctcgcgc gcgagccggc gatgacggat gaacagctcg ggcgcgcgct
     7141 cgccgccggc gccgaatcgg tcgcgcgcgc ccacgccgcc ggcgcgcaac tctatatcgg
     7201 cggcgagatg ggcatcggca acaccacagc gagcgcggcg ctggccgcgg cgctgctcga
     7261 ccagccggcc gccgcgctgg tcggccccgg caccggcgtc gatgccgacg ggttgcggcg
     7321 caagctcgcg gcggtcgagg cggcgctggc gcgtgcccgc acgagcgatc cgctcgccgc
     7381 gctgcgcgag ttcggcggct tcgaactggc ggcgctgacc ggcgcgtatc tggccgccgc
     7441 gcagcacggg ctgccggtgc tggtcgacgg cttcatcgcc tcggtcgccg cgctcgccgg
     7501 cttgcggctg caaccggcgc ttgccgactg gctgctgtac gcgcactgct cggccgagcc
     7561 cggtcaccgg cgcgtgctgg ccgcactcgg cgccgagccg ctgctcgacc tgggtctgcg
     7621 gctcggcgag gccagcggcg cggcggtggc cttacccttg ctgcgaatgg cgtgtgcctt
     7681 gcatgatgaa atggccacct tcgccgacgc cggggtatcg cgcgcatgaa ccgctacacg
     7741 accgtcatcg acctgatgcg ccatggcgaa ccggaaggcg gacgcctgtt ccgcggctcg
     7801 aaggacgacc cgctgtcagc gaccggctgg cagcagatgc gcacggtcgc ggccgagttc
     7861 agaccctggc aggcggtggt cagctcgccg ctgcgccgct gccgcgagtt cgccgaggaa
     7921 ctcgccacgc gccacggcct gccggtcgag atcgatccgc tgtttcgcga gatcggcttc
     7981 ggcgactggg agggcctgag cgccgagcag gtcgaggtcc gccaccccgg ggcgctggcc
     8041 gagtactggc gcgcgcccga aggctacacg ccgccgggcg gcgaaccgct ggtgcgcttc
     8101 gacgcgcgca tcgaagcggc ctgggacggc ctgctcgaac gccaccgcgg cgcgcacgtg
     8161 ctgctggtct gccacggcgg cgtgatccgc gcctgcctgc gtcaggtgct cgggctgccg
     8221 ctcgccaacc tctggcgcat cgacgtgccc tatgcggcac cgacccgcgt gcgcatccac
     8281 ggcgatcacg gcgaaccggt gccgatcctc gaattcctga acggcggctt cgccggctgg
     8341 tggctctgaa cgtccggcgg catcagttgc tgatgtcgcc gtcgaggtag cgccaggcac
     8401 catccacgcg tacgaagcgg ctggtctcgt gcatccggaa ggcgcgaccg ttgcgcttgc
     8461 cgcgcgcaac gaattcgacc gtgccttccg catcccctgc cccgccgccc tgcacggcca
     8521 gcaccttcag ccccagccac tgcaccggat cagcggccac atcgagcccg gccgggcagg
     8581 tgtccgggtg ccagctcgtg cgcagccagc gctcgtcgcg gcgcacgaac gcgctgtagc
     8641 gcgagcgcat cagcgcctcg gccgtcggcg gctcggcgct gccggcgagg taagggctgc
     8701 agcagtcggc gaacgcgcgc cccgagccgc aggggcaggc cgggtcgcag gccccggtac
     8761 ggcgactcat gccgggcccc gccggacgga cgccccgccg ggcgctgcgg gcgcgggaaa
     8821 ggcgtcagaa aaccttacat tcacgtgcct tcgaacctcg cattcattcc ccgcccgccc
     8881 ggccttcgcg gcgcgcatcg gcggccccga tccagccgcc acccgcggcg cggcggatcg
     8941 cctgcacgcc gctgttgagt tccaggatgc gcagctcatg accgagtgcg cgcagcgcgt
     9001 cggcctcggc cgtcagcgcg gtgccggcct ccagctcggt cgggccgttg cggctgccga
     9061 agttcggccg gt
//
LOCUS       DAOQIE010000150         9062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3890_length_9062_cov_8.513156, whole genome shotgun sequence.
ACCESSION   DAOQIE010000150 DAOQIE010000000
VERSION     DAOQIE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_9062_cov_8.513156"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK144_10395"
     CDS             <1..421
                     /locus_tag="PK144_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF66180.1"
                     /translation="PPAVEPPPARTQGRPPASAIAPPPVPAKPPARPRTPQPAAEPLA
                     RAPDTAWVVQLAATESETRLRSYAERNGLSGYVILRTRRNGQDWHVLLLGGFHPNQAA
                     AETALTRLPEALRSETTPWVRSVRSLRRLLPPADPAP"
     gene            679..5142
                     /gene="gltB"
                     /locus_tag="PK144_10400"
     CDS             679..5142
                     /gene="gltB"
                     /locus_tag="PK144_10400"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNF66181.1"
                     /translation="MQTNQQQGLYRPEFERDNCGFGLIAQMDDQASHWLVKTSIAALA
                     RLTHRGAVAADGKSGDGCGLLMKKPDGFLRIVAAEAGLTLGSLYACGLVFLNTDAQKA
                     QAARTLLETELGKEGLTVAGWRTVPTDPACCGEEALKTLPLFEQIFINAPEGMDPAQF
                     ERHLVVARRRTAYGLMDSDKAFYVVTLSAHVLGYKGLVMPAALPEFYKDLEDPRLESS
                     ICVFHQRFSTNTMPQWKLAQPFRYLAHNGEINTVQGNRNWALARGYTFSSPLLPMQDL
                     RPMVNMTGSDSSSLDNMLEVLLAGGMDVFRAMRLLVPPAWQNVDHLDPDLRAFYEYNS
                     MHMEPWDGPAGIVLTDGRYAACTLDRNGLRPARWVITKDRHMTIASEIGVYDYTPGEV
                     VAKGRVKPGEMLAVDTATGTLLSPADIDNLLKTRKPYRQWLAQNTKYLKSIFPKDVAS
                     SEPLKADELAVYEKLFQVSFEERDQVLRILAEGAQEAVGSMGDDTPFAVLSERARSPY
                     DYFRQQFAQVTNPPIDPLREAIVMSLEGCMGAEGNMFVEDELLSERLVISSPVLSEGK
                     FRAILKLNDGRYAHQRIDLNYPASEGLAQAVARFADEAVAAVQAGKVMIVLSDRSIEP
                     GRLPAHAALAVGAVHQRLVHEGLRCKANLMVETATARDPHQFAVLIGFGASMVYPYLA
                     FEVIEDMVRTGEITSKDTAKLAENYRKGINKGLFKIISKMGISTINSYRGAQLFEIVG
                     LHEEVVGLCFTGTTSRIQGANFADLEADVQALAAGAWRPRVAREQGGLLKYVHGGEYH
                     AYNPDVVQQLQAAVNSGDFKLWKVYSESVNTRPVATFRDLLQLKADGAKPVPLDQVEP
                     LDAILKRFDSAGMSLGALSPEAHETLAEAMNRLGGRSNSGEGGEDPARYGTVKVSKIK
                     QIASGRFGVTPAYLTSAEVLQIKVAQGAKPGEGGQLPGDKVDATIARLRFSRPGVALI
                     SPPPHHDIYSIEDLAQLIFDLKQVNPRALVSVKLVAEAGVGTIAAGVAKAYADLITIA
                     GYDGGTGASPLTSVKYAGSPWELGLTETHQTLRRNNLRDKVRLQTDGGLKTGLDVIKA
                     AILGAESFGFGTGPMIAMGCKYLRICHLNNCATGVATQNNVLRLNHYKGTVEKVMNYF
                     RFIAQEVRELMAMLGITRFTDLIGRTDLLEILPGSTGKHARLDLAPILSDGGVAADKP
                     RFCVEPHNAPFDKGELAEQMVRDTLAAIEAKRGGEFAYTVSNTVRSIGARLSGEIARR
                     YGDQGMNDAPVTLRLTGTAGQSFGVWNAGGLHMILEGDANDYVGKGMAGGKLVIHPPR
                     GSGFVEQDTTIIGNTCLYGATGGKLFAAGLAGERFAGRNSGAITVVEGVGDHGCEYMT
                     GGVVAVLGGTGVNFGAGMTGGFAFVLDEDNTFVDRYNHELIDIHRISTEAMEPHRHYL
                     RGLIEEFVAETGSPWGRQILADFRRYASKFWLVKPKAADLATLLDTLLQAA"
     gene            5223..6638
                     /locus_tag="PK144_10405"
     CDS             5223..6638
                     /locus_tag="PK144_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF66182.1"
                     /translation="MSKAKPMLFLDVSRQDPAKTPAEVRRHEFNEIYGQFDAKLAATQ
                     AGRCLHCGNPYCSWKCPVHNHIPHWLQLINEGNLFEAAELSHKTNSLPEICGRVCPQD
                     RLCEGACTLNDGFGAVTIGSVEKYITDEAIKAGWRPKLDHVKPTGKKVAIIGAGPAGL
                     GCADILVRNGVKAVVYDRHPQIGGLLTFGIPPFKLEKDVLQTRRELMEGMGVEFRLGV
                     EVGTDLSFQSLLDEYDAVFLGMGTYTYMKGGFPGEDLAGVHDALPFLISNINRLLGYE
                     RSAADFIDVAGQRVVVLGGGDTAMDCNRTSIRQGATSVTCAYRRDEANMPGSKREVAN
                     AREEGVKFLWNRQPVEIVGNGRVEGVKVVTTRLGAPDARGRRSPEVVPGSEEIIAADT
                     VLIAFGFRPDPKPWFAEFGIATDDRGRIAVRAAGSERQFQTGNPKVFAGGDMVRGSDL
                     VVTAVFEGREAALGILDYLEV"
     gene            6763..7842
                     /gene="hemE"
                     /locus_tag="PK144_10410"
     CDS             6763..7842
                     /gene="hemE"
                     /locus_tag="PK144_10410"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNF66183.1"
                     /translation="MNPPLKNDRFLRALLREPVDATPVWMMRQAGRYLPEYRATRARA
                     GSFMSLCTAPELACEVTLQPLARFPLDAAILFSDILTVPDAMGLGLSFAEGEGPRFAH
                     PVRSAAEIERLGVPDPELELGYVMDAVRLIRRELAGRVPLIGFSGSPWTLATYMVEGG
                     SSKEFAKVKGLMYEQPQVLHKLLGTVTRAVIAYLNAQIGAGAQAVMVFDTWGGALSPA
                     TYRSFSLDYMARIVEGLNREAEGRRVPVILFTKGGGAWLEAMADTGCDALGLDWTTDI
                     GEARARVGSRVALQGNLDPCVLYASPERIRAEVGQVLAGYGRGSGHVFNLGHGIHPHV
                     DPEHAGAMVAAVHELSRAYHGGQTD"
     gene            7859..8371
                     /locus_tag="PK144_10415"
     CDS             7859..8371
                     /locus_tag="PK144_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF66184.1"
                     /translation="MDQQQTSNPARGGRRILSCGVIVLRASAGGSGGNWEYLLLRAFN
                     YWDFPKGMREPGEHPLSTALREVREETTIDSLDFRWGEVFRETPPYNHGCKVARYYLA
                     VAPADATVDLPVSPELGRPEHSEFRWVTRREAWSLLTPRVRAILRWSDTILSRPPRQD
                     APAPLPSPAD"
     gene            8506..>9062
                     /locus_tag="PK144_10420"
     CDS             8506..>9062
                     /locus_tag="PK144_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF66185.1"
                     /translation="MQTHKYRPLKFRHLDTGVTCFRPRQHLPDRITQDSPATDAMTDL
                     RQVSAQMVSLQTPMNVALERMIKGGVRLLLVADPDGTIRGVITSRDIQGEKPMKLMEK
                     TGTKREELTVRDIMTLYPRLEVLDITDVLRATVGDIIATLREQGRQHALVVDTDPDSG
                     KEAVRGIFSLSQIGQQLGLEIHPQAR"
BASE COUNT         1481 a         3301 c         2986 g         1294 t
ORIGIN      
        1 gccgccggcc gtggagccgc cgccggcccg cacgcagggc cggccaccgg cttccgccat
       61 cgccccgccg cccgtgcccg caaaaccgcc ggcacgtccg cgcacgccgc agccggcggc
      121 ggagccgctc gcgcgcgcac cggatacggc ctgggtggtg cagctcgccg cgaccgaatc
      181 cgaaacgcgg ctgcgcagct acgccgaacg caacggcctc agcggctacg tgatcctgcg
      241 cacccggcgc aacggccagg actggcacgt gctgctgctc ggcggtttcc atccgaatca
      301 ggcggcggcc gaaacggccc tgacccgcct gcccgaggcg ctgcgcagcg aaaccacgcc
      361 ctgggtgcgc agcgtgcgtt cgctgcgccg gctgctgccg cccgccgatc ccgcgccctg
      421 acggcgcggg ctgcaccgtt tcggcgcacc cgcatcgggc atgcgcgatt gagtcaggct
      481 gcgggaaaag tcaaggcttc gctgtccgct tacgtttacc gatgcggcac tgcaccgtta
      541 tactgcgcgg tccgcgtcac tggccgccgg catgaatcct gctcataccg gctccgatgc
      601 gcagccagcc ccccacggtc cggcatcccc ggccggccgt cacccacgtt agcggcatac
      661 tctgcaggcg gagcaaagat gcagacgaat cagcagcagg gcctgtaccg gcccgaattc
      721 gagcgcgaca actgcggctt cggcctcatc gcgcagatgg acgatcaggc cagccactgg
      781 ctcgtcaaga cctccatcgc cgcgctcgcc cgcctgaccc accgcggcgc ggttgccgcc
      841 gatggcaagt ccggtgacgg ctgcggcctg ctgatgaaga aacccgacgg cttcctgcgc
      901 atcgtcgccg ccgaggccgg cctgacgctg ggctcgctgt atgcctgcgg cctggtgttc
      961 ctgaacaccg atgcgcagaa ggcgcaggcc gcgcgcacgc tgctcgaaac cgaactcggc
     1021 aaggaaggcc tgacggtcgc cggctggcgc accgtgccga ccgatcccgc ctgctgcggc
     1081 gaggaagccc tgaagacgct gccgctgttc gagcagatct tcatcaacgc ccccgaaggc
     1141 atggacccgg cgcagttcga gcgccacctg gtcgtcgccc gccggcgcac ggcctacggg
     1201 ctgatggaca gcgacaaggc cttctacgtc gtgacgctgt cggcgcatgt gctcggctac
     1261 aagggtctgg tgatgccggc ggcgctgccg gagttctaca aggatctcga agacccgcgg
     1321 ctggaatcgt cgatctgcgt gttccaccag cgcttctcga ccaacacgat gccgcagtgg
     1381 aagctcgcgc agccgttccg ctacctcgcg cacaacggcg agatcaacac ggtccagggc
     1441 aaccgcaact gggcgctggc gcgcggctac acgttctcgt cgccgctgct gccgatgcag
     1501 gacctgcggc cgatggtcaa catgaccggc tcggactcca gctcgctcga caacatgctc
     1561 gaagtgctgc tcgccggcgg catggacgtg ttccgtgcga tgcgcctgct ggtgccgccg
     1621 gcctggcaga acgtcgatca cctcgatccg gacctgcgcg cgttctacga gtacaactcc
     1681 atgcacatgg agccgtggga cggcccggcc ggcatcgtgc tgaccgacgg ccgctacgcc
     1741 gcgtgcacgc tcgatcgcaa cggcctgcgg ccggcgcgct gggtgatcac gaaggaccgg
     1801 cacatgacga tcgcctcgga gatcggcgtc tacgactaca cgcccgggga ggtcgtcgcc
     1861 aagggccgcg tcaagcccgg cgagatgctg gccgtcgaca ccgcgaccgg cacgctgctc
     1921 agccccgccg acatcgacaa cctgctgaag acgcgcaagc cctaccgcca gtggctcgcg
     1981 cagaacacca agtacctgaa gtcgatcttc ccgaaggatg tcgccagctc cgagccgctg
     2041 aaggccgatg aactcgccgt ctacgagaag ctcttccagg tcagcttcga ggagcgcgac
     2101 caggtgctgc gcatcctcgc cgagggcgcg caggaagccg tcggctcgat gggcgacgac
     2161 acgccgttcg cggtgctctc cgagcgcgcg cgttcgccgt acgactactt ccgccagcag
     2221 ttcgcgcagg tcacgaaccc gccgatcgat ccgctgcgcg aagcgatcgt catgtcgctc
     2281 gaaggctgca tgggcgccga aggcaacatg ttcgtcgagg acgagctgct gtccgaacgc
     2341 ctcgtgatct cctcaccggt gctctccgaa ggcaagttcc gcgcgatcct gaagctcaac
     2401 gacggccgct acgcgcacca gcgcatcgac ctgaactacc cggcctccga aggtctggcg
     2461 caggcggtcg cgcgcttcgc cgacgaggcg gtcgccgccg tgcaggccgg caaggtcatg
     2521 atcgtgctgt ccgaccgctc gatcgagccg ggccggctgc cggcgcacgc cgcgctcgcc
     2581 gtcggtgccg tgcaccagcg cctcgtgcac gagggcctgc gctgcaaggc caacctgatg
     2641 gtcgagaccg cgaccgcccg cgacccgcac cagttcgcgg tgctgatcgg cttcggcgcg
     2701 tcgatggtct acccctacct ggccttcgag gtcatcgagg atatggtccg caccggcgag
     2761 atcacctcga aggacacggc caagctcgcc gagaactacc gcaagggcat caacaagggc
     2821 ctgttcaaga tcatctcgaa gatgggcatc agcaccatca acagctaccg cggcgcgcag
     2881 ctgttcgaga tcgtcggcct gcacgaggaa gtcgtcgggc tctgcttcac cggcaccacc
     2941 agccgcatcc agggcgcgaa cttcgccgac ctcgaagccg acgtgcaggc gctcgccgcc
     3001 ggtgcctggc gcccgcgcgt ggcgcgcgag cagggcggac tgctgaagta cgtgcacggc
     3061 ggcgagtacc acgcctacaa cccggacgtg gtccagcagc tgcaggccgc cgtcaacagc
     3121 ggcgacttca agctctggaa ggtttattcc gagtccgtca acacccggcc ggtcgcgacc
     3181 ttccgcgacc tgctgcagct gaaggccgat ggtgcgaagc cggtgccgct cgatcaggtc
     3241 gagccgctcg atgcgatcct caagcgcttc gactcggccg gcatgtcgct cggcgcgctg
     3301 tcgcccgagg cgcacgagac gctcgccgaa gccatgaacc gcctcggcgg gcgctcgaac
     3361 tccggcgagg gcggcgagga cccggcgcgc tacggcacgg tgaaggtctc gaagatcaag
     3421 cagatcgcct cggggcgctt cggcgtgacg ccggcgtatc tcaccagcgc cgaggtgctg
     3481 cagatcaagg tcgcgcaggg cgccaagccc ggcgagggcg gccagctgcc cggtgacaag
     3541 gtcgatgcga cgattgcccg gctgcgcttc tcgcgcccgg gtgtcgcgct gatctcgccg
     3601 ccgccgcacc acgacatcta ctcgatcgag gacctcgcgc agctgatctt cgacctcaag
     3661 caggtcaacc cgcgggcgct ggtgtcggtg aagctcgtcg ccgaggccgg tgtcggcacg
     3721 atcgccgccg gtgttgccaa ggcctacgcc gacctcatca cgatcgccgg ctacgacggc
     3781 ggcaccggtg cctcgccgct gacctccgtc aagtacgccg gcagtccgtg ggagctcggc
     3841 ctgaccgaga cgcaccagac gctcaggcgc aacaacctgc gcgacaaggt gcggctgcag
     3901 accgacggcg gcctgaagac cggcctcgat gtcatcaagg ccgcgatcct cggcgccgag
     3961 agcttcggct tcggcaccgg cccgatgatc gcgatgggct gcaagtacct gcgcatctgc
     4021 cacctgaaca actgcgcgac cggcgtggcg acgcagaaca acgtgctgcg cctgaaccac
     4081 tacaagggca cggtcgagaa ggtgatgaac tacttccggt tcatcgccca ggaagtccgc
     4141 gagctgatgg ccatgctcgg catcacgcgc ttcacggacc tcatcggccg caccgacctg
     4201 ctggagatcc tgcccggcag caccggcaag cacgcgcgcc tcgatctggc accgatcctc
     4261 tccgacggcg gcgtggcggc cgacaagccg cgcttctgcg tcgagccgca caacgcgccg
     4321 ttcgacaagg gcgagctggc cgagcagatg gtgcgcgaca cgctggcggc gatcgaggcg
     4381 aagcgcggcg gcgagttcgc gtacacggtc agcaacaccg tgcgctcgat cggtgcgcgc
     4441 ctctccggcg agatcgcgcg ccgctacggc gaccagggca tgaacgacgc gccggtcacg
     4501 ctgcgcctga ccggcaccgc cggccagagc ttcggcgtct ggaatgccgg cggcctgcac
     4561 atgatcctcg aaggcgacgc caacgattac gtcggcaagg gcatggccgg cggcaagctg
     4621 gtcatccacc cgccgcgcgg ctcgggcttc gtcgagcagg acacgacgat catcggcaac
     4681 acctgcctgt acggtgcgac cggcggcaag ctgttcgcgg cggggctcgc cggcgagcgc
     4741 ttcgcggggc gcaactccgg cgcgatcacc gtcgtcgagg gcgtcggtga ccacggctgc
     4801 gagtacatga ccggcggcgt ggtcgcggtg ctcggcggca ccggcgtcaa cttcggcgcc
     4861 ggcatgaccg gcggcttcgc gttcgtgctc gacgaggaca acaccttcgt cgatcgctac
     4921 aaccacgagc tgatcgacat ccaccgcatc tcgaccgagg cgatggaacc gcaccgccac
     4981 tacctgcgcg gactgatcga ggagttcgtc gccgagaccg gcagcccgtg gggccggcag
     5041 atcctcgccg acttccgccg ctatgcgtcg aagttctggc tggtcaagcc gaaggccgcc
     5101 gatctggcca cgctgctcga cacgctgctg caggccgcct gacgcgctct gccgcgcacc
     5161 ggggccccgg ccccggtgcc gcggtgcctg cccgatatcg cctctcaccc aagcggatcg
     5221 tcatgtccaa agccaaaccg atgctgttcc tcgacgtgag ccgtcaggat ccggcgaaga
     5281 cgccggccga agtccgccgg cacgaattca acgaaatcta cggccagttc gacgccaagc
     5341 tggcggcgac ccaggccggg cgctgcctgc actgcggcaa cccgtactgc tcgtggaagt
     5401 gcccggtgca caaccacatc ccgcactggc tgcagctgat caacgaaggc aacctgttcg
     5461 aggcggccga gctgtcgcac aagaccaact cgctgcccga gatctgcggc cgcgtctgcc
     5521 cgcaggatcg cctgtgcgaa ggcgcctgca cgctgaacga cggcttcggc gcggtcacga
     5581 tcggttcggt cgagaagtac atcaccgacg aggcgatcaa ggccggctgg cggccgaagc
     5641 tcgatcacgt caagcccacc ggcaagaagg tcgcgatcat cggggccggc ccggccggcc
     5701 tcggctgcgc cgacatcctc gtgcgcaacg gcgtcaaggc cgtcgtctac gaccgccatc
     5761 cgcagatcgg cggcctgctg accttcggca tcccgccgtt caagctcgaa aaggacgtgc
     5821 tgcagacccg gcgcgagctg atggaaggca tgggtgtcga gttccggctc ggcgtcgagg
     5881 tcggcaccga cctgagcttc cagagcctgc tcgacgagta cgacgccgtg ttcctcggca
     5941 tgggcacgta cacgtacatg aagggcggct tcccgggcga ggacctcgcc ggcgttcatg
     6001 acgcgctgcc cttcctgatc tcgaacatca accgcctgct cggctacgag cgcagcgccg
     6061 ccgacttcat cgatgtcgcc ggccagcgcg tcgtcgtgct cggcggcggc gacacggcga
     6121 tggactgcaa ccgcacctcg atccgccagg gcgcgaccag cgtcacctgc gcgtaccgcc
     6181 gcgacgaggc caacatgccc ggctcgaagc gcgaggtcgc caacgccagg gaagaaggcg
     6241 tgaagttcct ctggaaccgc cagccggtcg agatcgtcgg caacggccgg gtcgagggcg
     6301 tcaaggtggt caccacccgg ctcggcgcgc ccgatgcccg cggccggcgc agccccgagg
     6361 tcgtgcccgg ctccgaggag atcatcgcgg ccgacaccgt gctgatcgcc ttcggcttcc
     6421 gtccggaccc gaagccgtgg ttcgccgagt tcggcatcgc caccgatgat cgcggccgca
     6481 tcgccgtgcg cgccgccggc agcgagcgcc agttccagac cggcaacccg aaggtgttcg
     6541 ccggcggcga catggtgcgc ggttccgatc tggtcgtgac cgcggtcttc gaaggccgcg
     6601 aagcggcact cggcatcctc gactacctcg aagtctgagc ctcccgcccc ccgcagcggc
     6661 tgccgcaagc ctccggcggc cgctgcggcg gcgcatcgtg gccgatcggc gcgcgatgcg
     6721 gcacactgcc ggcctttccg cgccgtgttg ccgagcccgc cgatgaaccc gcccctgaag
     6781 aacgaccgat tcctgcgcgc gctgctgcgc gaacccgtcg atgcgacgcc ggtctggatg
     6841 atgcgtcagg ccggccgcta cctgcccgag taccgcgcca cgcgcgcgcg cgccggcagc
     6901 ttcatgtcgc tctgcaccgc tccggagctg gcctgcgagg tcacgctgca gccgctcgcg
     6961 cgcttcccgc tcgatgcggc gatcctgttc tccgacatcc tgaccgtgcc cgatgcgatg
     7021 gggctcggcc tgtccttcgc cgagggcgag gggccgcgct tcgcgcaccc ggtgcgcagc
     7081 gccgccgaga tcgaacgcct cggcgtgccc gatcccgaac tcgaactcgg ctacgtgatg
     7141 gacgccgtgc gcctgatccg ccgcgaactc gccggtcgcg tgccgctgat cggcttctcc
     7201 ggcagcccgt ggacgctcgc gacctacatg gtcgaaggcg gctccagcaa ggagttcgcg
     7261 aaggtcaagg gcctgatgta cgaacagccg caagtgctgc acaagctgct cggcacggtc
     7321 acgcgcgcgg tcatcgccta cctgaacgcg cagatcggcg ccggtgcgca ggcggtgatg
     7381 gtgttcgaca cctggggcgg tgcactgtcg ccggccacct accgcagctt ctcgctcgac
     7441 tacatggcgc gcatcgtcga aggcctcaac cgcgaggccg aaggccggcg cgtgccggtg
     7501 atcctgttca cgaagggcgg cggagcctgg ctcgaagcga tggccgacac cggctgcgat
     7561 gcgctcggcc tcgactggac caccgacatc ggcgaggccc gtgcccgcgt cggcagccgc
     7621 gtggcgctgc agggcaacct cgatccctgc gtgctgtacg cctcgcccga gcgcatccgc
     7681 gccgaggtcg gtcaggtgct cgccggctac ggtcgcggca gcggtcacgt cttcaacctc
     7741 ggccacggca tccacccgca cgtcgatccg gaacacgccg gcgcgatggt cgccgcggtc
     7801 cacgaactgt cgcgcgccta tcacggcggc cagaccgact gagacgacag ggagcgtcgt
     7861 ggaccagcag cagacctcga acccggcccg tggcggccgc cgcatcctgt cctgcggcgt
     7921 catcgtgctg cgcgcgtccg ccggcggcag cggcggcaac tgggaatatc tgctgctgcg
     7981 cgccttcaac tactgggact tcccgaaagg catgcgcgag cccggcgagc acccgctcag
     8041 cacggcgctg cgcgaggtgc gcgaggaaac cacgatcgac tcgctcgatt tccgctgggg
     8101 cgaggtgttc cgcgagacac cgccgtacaa ccacggctgc aaggtcgcgc gctattacct
     8161 ggccgtggcc ccggccgatg ccacggtcga cctgccggtc agccccgagc tcggccggcc
     8221 cgagcacagc gaattccgct gggtgacgcg gcgcgaggcc tggagcctgc tgacgccgcg
     8281 cgtgcgcgcg atcctgcgct ggtccgacac catcctgagc cgtccgccgc gccaggacgc
     8341 gcccgcgccg ctgccgtcgc ccgccgactg aaattcacat taagcccgcc ctgcggcgcg
     8401 gtcgggccga tttacagttc cggcccccgc gtctatgcct atggcccgcg tccccaagaa
     8461 cgaagcccag gcagttcaag accaccgccc cggaggcccg ccatcatgca gactcacaag
     8521 taccgcccgc tgaaattccg ccacctcgac accggggtca cctgcttccg cccgcgccag
     8581 catctgcccg atcgcatcac gcaggacagc ccggcgaccg acgcgatgac cgacctgcgt
     8641 caggtcagcg cccagatggt gtcgctgcag acgccgatga acgtcgcgct ggaacgcatg
     8701 atcaagggcg gcgtgcgcct gctgctggtg gccgacccgg acggcacgat ccgcggcgtc
     8761 atcaccagcc gcgacatcca gggcgagaag ccgatgaagc tgatggagaa gaccggcacg
     8821 aagcgcgagg aactgaccgt acgcgacatc atgacgctgt acccgcgcct cgaagtgctc
     8881 gacatcaccg acgtgctgcg cgccacggtc ggcgacatca tcgcgacgct gcgcgagcag
     8941 ggccgccagc acgcgctggt ggtcgacacc gatccggaca gcggcaagga ggcggtgcgc
     9001 ggcatcttct cgctgtcgca gatcggccag cagctcggcc tcgaaatcca cccgcaggcg
     9061 cg
//
LOCUS       DAOQIE010000151         8967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3949_length_8967_cov_7.832810, whole genome shotgun sequence.
ACCESSION   DAOQIE010000151 DAOQIE010000000
VERSION     DAOQIE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_8967_cov_7.832810"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PK144_10425"
     CDS             <1..1161
                     /locus_tag="PK144_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF66186.1"
                     /translation="EALEIRNFRLLRELRLSGLPRFGALVGVSGSGKSMLFDVFAFLR
                     EAMLGDVTAAVARRGGYRALAAPGMAGEPVELSLRMRLPPGGQEQVLCYTLRFEADAG
                     GRPVIVREKLYQEPPADGGGAEFIEVLRERTYAIACVGADAPDGAEPRYEEQSLHAPD
                     TLAVAALGQFRRFAAASALRAAILGWRVVDLDACARPHAAPAEVGADPASLAQAAGRL
                     HAQQPERFALAVAALRQRVPGLAAVEVETGRDGRCTLRFRDAHGEEAFDVQQLAEGTR
                     RLFAWLLLLHEVPPHSLLALETPECRLHPDLLPELADLLRAASLQGTQVLIATHAPAL
                     LNALTLDEILCLRRDASGVSVTCAGASEDLRALYEAGEAPGRLWAQGLFEAL"
     gene            complement(1410..1829)
                     /locus_tag="PK144_10430"
     CDS             complement(1410..1829)
                     /locus_tag="PK144_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66187.1"
                     /translation="MKTMQTPAATSAAVLRSRSSRLRLAALLAPVVMAGTLAACSSDP
                     VIVQQPIERERVVVREVPQSPTVIVPQAPPATRTEVIPPAPSMQHVWVPGAWVWRDGW
                     VWQAGHYEQRPYANARWIDGRWDSTPAGWRWTPGHWG"
     gene            complement(1945..2361)
                     /locus_tag="PK144_10435"
     CDS             complement(1945..2361)
                     /locus_tag="PK144_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66188.1"
                     /translation="MNKTLIAATVLSLSSGLALAAGTTSDDARAPAIQPVPGTESPAR
                     TPQTDGREAVPPRDKVAKGEPFKTFDDNHDGLISGDELEDIKDLDLARVDRNHDGHIS
                     PAEYEATGPFEQKSLIQKAKDALSSIGDSAETRTTH"
     gene            complement(2617..3078)
                     /locus_tag="PK144_10440"
     CDS             complement(2617..3078)
                     /locus_tag="PK144_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNF66189.1"
                     /translation="MPYWLMKSEPEAFSLDALAAAPAQTTHWDGVRNYQARNWMRDRM
                     RPGDGVFFYHSNTAQPGIVGLAEVTSPGYPDHTAWTPGHVHEDPKSTPAQPLWYMVDI
                     RYVRHLRRTISLTELKTHAGALGDFPLLRRGNRLSVMPVGDAQWTYILGLE"
     gene            complement(3195..3905)
                     /locus_tag="PK144_10445"
     CDS             complement(3195..3905)
                     /locus_tag="PK144_10445"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HNF66190.1"
                     /translation="MLNLRTSLAGTLGSFLLLSAAEAATLSAGVQNFDYQYDYAGAGF
                     IGGGNGGLLPFAFFNPALGTLTGVRFTLDSRHTATITVSATNLSPQDNLAVQGGYSGD
                     VFMDLLDPNLVYGTLYSEVLSLGAGCDTGAPGSCSASDSFNLVLANVNDIDFTYFALF
                     EGTGSYSVSMNVGGQLFAEQVFPGSGPATGSGSGRWFGTLSVVYEYDPAATAIPVPAT
                     PALVALGLGALARLRYRR"
     gene            complement(4030..4635)
                     /locus_tag="PK144_10450"
     CDS             complement(4030..4635)
                     /locus_tag="PK144_10450"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003303307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNF66191.1"
                     /translation="MTEETASAIDVIALRRRLRAARRALSPAAQQAAAEAVAVRIRAL
                     PAYAAARSIAVYLACDGELDPAVLVAGARRDGRTLLLPRLLPEAGRMDFAEWPADAPL
                     VANRFGIPEPEGTPLHAPEAVDLVLTPLVGFDAHGGRLGMGGGYYDRSFAFLLARPRP
                     SRPYLVGLAHACQRVDRLEVRPWDVPLDAVVSDTGGYPQAA"
     gene            complement(4858..5169)
                     /locus_tag="PK144_10455"
     CDS             complement(4858..5169)
                     /locus_tag="PK144_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66192.1"
                     /translation="MTTSSAALIAALLGIALNNYSAEATEPAQDGAPTTQSTRTSQTS
                     QPSQTLPSPVNAPAARPQMLPTANPASGETVRPVKRQMAPLRPGDRFAWLETALQHVA
                     A"
     gene            complement(5523..6263)
                     /gene="phbB"
                     /locus_tag="PK144_10460"
     CDS             complement(5523..6263)
                     /gene="phbB"
                     /locus_tag="PK144_10460"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNF66193.1"
                     /translation="MARVAVVTGGMGGLGEAICIKLAALGDKVVATYSPGNKTAEEWL
                     AGMKAQGFDFSAVPVDVTDFDSCAACVKQVEADIGPIDVLVNNAGITRDMTFKKMTKA
                     DWDAVIKTNLDSVFNMTKQVMDGMMARNWGRVVNISSVNGIKGAFGQTNYAAAKAGMH
                     GFTKALALEVCKKGVTVNTISPGYIGTKMVRAIPQEVLDTKILPLIPMGRLGEPNEVA
                     GLVAYLCSEEAAFLTGANISINGGQHMF"
     gene            6650..7852
                     /gene="ilvA"
                     /locus_tag="PK144_10465"
     CDS             6650..7852
                     /gene="ilvA"
                     /locus_tag="PK144_10465"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNF66194.1"
                     /translation="MISLDDIIKARRRIRDSLPPTPCARAAQLSRQLGCELFIKCENL
                     QMTGSFKVRGALARILQLTEAERAAGVIAASAGNHAQGVAWAAQKFGVPATIVMPETA
                     PLAKIRGTAAFGADIVLAGTGYDAAYARAVELQRDSGATFIHAFDDPAVMAGQGSAGL
                     EIIEQLGDFDLVLVPVGGGGLAGGIATVIKQTRPGVQVIGVQSARVPSMQASLAAGAP
                     TPVPPAATLADGIAVARVGVHTYPQAAAWLDGIVTVDEESIARAILTLLEQEKLLAEG
                     AGAVGIAALLDGVVGDPAGKRIVAVVSGGNIDPIELAKIVERGLERDGRLAHLRVVVA
                     DRPGSIAEVAGLVARERANILQISQRRDAAEVGLREAELDLTLETRGLDHVTGIVQAL
                     RDGGFRVR"
     gene            7917..>8967
                     /locus_tag="PK144_10470"
     CDS             7917..>8967
                     /locus_tag="PK144_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66195.1"
                     /translation="MSDSVPAPLTAAEVRAVVLGSVLALFLAALDQSIVATALPAIAA
                     ELGRVELMSWVVTAYLLASTCATPVLGKASDVLGRRRVLAGCLIVFLGGSVLCSLATS
                     MPMLIAARALQGLGGGGLITLAQATVADVVPPRERGRYVAYFASVWACAGLLGPTLGG
                     LLTQYLGWPWIFWINLPLGLAALVITDRVLRRLPVRGGTRGRIDLPGIVLLSTATTAL
                     LLALSFGGHRWPWASAPILGLLALAVVPALLFAHRQRHTAEPLLPPAFFRDPVVAPTG
                     AALFLVFGGFLAVAVLVPAWLQIGLGRSAAAAGLLMMPMSLAAPLTATLTGHYLRATG
                     RYRRPPQLGLPLAAVA"
BASE COUNT         1251 a         3086 c         3210 g         1420 t
ORIGIN      
        1 gaagccctcg aaatccgcaa cttccgcctg ctgcgtgagc tgcgcctgtc cgggctgccg
       61 cgtttcgggg cgctggtcgg tgtcagcggc agcggcaagt cgatgctgtt cgacgtgttc
      121 gcgttcctgc gcgaagccat gctcggcgat gtcacggctg ccgtggcgcg gcgcggcggc
      181 taccgggcgc tggctgcgcc gggcatggcc ggggagccgg tcgagctgtc cttgcggatg
      241 cggctgccgc cgggcgggca ggagcaggtg ctctgctaca cgctcaggtt cgaagccgac
      301 gccggcgggc ggccggtgat cgtgcgcgag aagctctatc aggaaccgcc ggccgatggc
      361 ggcggggccg agttcatcga ggtgctgcgc gagcgcacct acgccatcgc ctgtgtcggg
      421 gccgatgcgc cggatggcgc cgagccgcgc tacgaggagc agtcgctgca cgcgccggac
      481 acgctcgcgg tcgcggccct cggccagttc cggcgctttg cggccgcgag cgcgctgcgg
      541 gcggcgatcc tcggctggcg ggtggtcgat ctcgatgcct gcgcgcgccc gcacgcggcc
      601 ccggccgagg tcggcgccga tcccgcgtca ctggcgcagg ccgccgggcg tctgcacgcg
      661 cagcagcccg agcgtttcgc gctggccgtc gcggcgctgc gccagcgggt gccggggctg
      721 gcggcggtgg aggtcgaaac cggccgggat ggccgctgca cgctgcgctt tcgcgacgcg
      781 cacggcgagg aagccttcga cgtgcagcag ctcgccgaag gcacgcgccg cctgttcgcc
      841 tggctgctgc tgctgcacga ggtcccgccg cattcgctgc tggccctcga aacccccgag
      901 tgccgcctgc atcccgacct gctgcccgaa ctcgccgacc tgctgcgcgc cgcgagcctg
      961 cagggcacgc aggtgctgat cgccacgcac gcgccggccc tgctcaatgc gctgacgctc
     1021 gatgaaatcc tctgcctgcg ccgcgacgcc agcggcgtgt cggtgacgtg cgccggcgcc
     1081 tccgaggact tgcgcgcgct gtacgaagcc ggcgaggcgc cggggcggct gtgggcgcag
     1141 ggcttgttcg aggcgctgtg agtcgcacgg ggcatcggcg gactcgaagc ctcgatccgc
     1201 gacgccctgg tgccggcgct gctgctcgat tggctgcgcc acttggtatg gaaaaggagg
     1261 cgagggctct ttgtcgcaca aagcgcccgt caacccggct tgcgtgccgg aaagcccatc
     1321 tgcagccctt cccccgagag ggggaagggt gaaaagcggt tgccggatca gcgccttgca
     1381 aggtccggcc accttcagtc gcgaaggact cagccccagt ggcccggcgt ccagcgccat
     1441 ccggccggcg tcgaatccca gcggccatcg atccagcgcg cgttcgcata cgggcgctgc
     1501 tcgtagtggc cggcctgcca gacccagccg tcgcgccaca cccaggcgcc cggcacccag
     1561 acgtgctgca tcgacggggc cggcggaatg acttcggtgc gcgtcgccgg cggcgcctgc
     1621 ggcacgatga cggtcgggga ctgcggcacc tcgcgcacga cgacgcgctc gcgctcgatc
     1681 ggctgctgga cgatgaccgg gtccgacgag cacgcggcca gcgtgccggc catcacgacc
     1741 ggcgccagca gcgccgccag gcgcagccgg gaggaacggg aacggagcac ggcggcggat
     1801 gtggcggcag gggtttgcat cgttttcatt ttttatcgac tcctcggcag gaagaaccgg
     1861 ccccgggggg caggccggcg ccttgtccgg cctgacgcgg cggggcgggg ggaaccctca
     1921 ccgcgtcagg ccggacacgg ggactcagtg cgtggtgcgg gtctcggcgc tgtcgccgat
     1981 cgagctgagc gcgtccttgg ccttctggat caggctcttc tgctcgaacg gtccggtggc
     2041 ttcgtattcg gccgggctga tgtggccgtc gtggttgcga tcgaccctgg cgagatccag
     2101 gtccttgatg tcctcgagtt cgtcgccgga gatcaggccg tcgtggttgt catcgaaggt
     2161 cttgaacggc tcgcccttgg cgaccttgtc gcgcggcggg acggcttcgc gaccatcggt
     2221 ctgcggcgtg cgcgccgggc tttcggtgcc tggcaccggc tggatggccg gcgccctggc
     2281 gtcgtcgctg gtggtgccgg cggccagcgc caggccggaa ctcagggaca atacggtggc
     2341 agcgatcagg gtcttgttca tctcacacct cactggacag cggtcggtgg tggaaggccc
     2401 gcagtgccgg accggtgacc gaagccagag caggcaggat gccagcctga tcaattcatc
     2461 gtgaattcat gcacttgatg agtttccgcc ttccgcgtgg atgagacggc tgcgcttgat
     2521 gctgtcgccc gagggcgacg aaatttccct gtcctgagca aggtcatgaa cgtcgtgccc
     2581 gggccgcagg ccgcgccggc cgcggacagg catggctcat tcgagtccga ggatgtacgt
     2641 ccactgggcg tcgccgaccg gcatcaccga cagccggttg ccgcggcgca gcagcggaaa
     2701 atcgccgagc gctccggcgt gcgtcttcag ttcggtcagc gaaatcgtgc gccgcagatg
     2761 gcgcacgtag cggatgtcga ccatgtacca gagcggctgc gccggggtgc tcttcgggtc
     2821 ttcgtgcaca tggccggggg tccacgcggt gtggtccgga tagccggggc tcgtgacctc
     2881 ggcgaggccg acgatgcccg gctgtgcggt gttcgagtgg tagaagaaca cgccgtcgcc
     2941 ggggcgcatc cggtcgcgca tccagttgcg cgcctggtaa ttgcgcacgc cgtcccagtg
     3001 cgtggtctgc gccggggcgg cggcgagggc atcgaggctg aaggcctcgg gttcggactt
     3061 catcagccag tacggcatgc gcttgactcc ggcgggcgac agggcggggc cggattctac
     3121 cggtccgccc gcaggcgtcg ctgccgccgg atcacgccat cgcgcctgcc ccggtcagcg
     3181 ggcgccgcgg cgcctcagcg ccggtaacgc agccgggcca gcgcaccgag tccgagggcc
     3241 accagcgccg gggttgccgg caccggaatc gccgttgcgg ccggatcgta ttcgtacacg
     3301 accgacagcg tgccgaacca gcgaccgctg ccgctgccgg tggccggccc ggagcccgga
     3361 aacacctgct cggcgaacag ctggccgccg acgttcatgc tgaccgaata gctgcccgtg
     3421 ccctcgaaca gggcgaaata cgtgaagtcg atgtcgttga cgttggcgag cacgagattg
     3481 aacgaatcgc tggccgaaca gctccccggt gcgccggtat cgcaaccggc accgagtgac
     3541 aggacttcgg aatacagcgt gccgtagacg agattcggat cgagcaggtc catgaacacg
     3601 tcgccggaat agccgccctg taccgccagg ttgtcctgcg gcgaaagatt ggttgccgac
     3661 acggtgatcg tcgccgtatg gcggctgtcg agggtgaagc gcacgccggt cagcgtgccg
     3721 agcgccggat tgaagaacgc gaacggcagc aggccgccgt tgccgccgcc gatgaaaccg
     3781 gccccggcgt agtcgtactg ataatcgaag ttctggacac ccgccgacag cgtggcggcc
     3841 tcggccgcgc tcagcagcag gaagctgccg agcgtgccgg caagggaagt gcggaggttc
     3901 aacatcgggc gatctcctca gttcgtcgtt atgccgcacc ggcaacggac cggatgcagc
     3961 tcgtccgtcg gagcgcgcgt ggcggcggcg ctgcgcggac gaatcccgag cgtagaagag
     4021 tcgcgcgctt caggccgcct gcggataccc cccggtgtcg ctgaccacgg catcgagcgg
     4081 cacgtcccag ggacggactt cgagccggtc gacgcgctgg caggcatggg cgagaccgac
     4141 gagatacggc cgcgacgggc gcggtcgcgc cagcaggaac gcgaagctgc ggtcgtaata
     4201 gccgccgccc atgccgagcc ggccgccgtg cgcatcgaag ccgaccagcg gcgtcagcac
     4261 cagatcgacg gcttcggggg cgtgcagcgg cgtgccctcc ggctccggga tgccgaagcg
     4321 gttcgccacg agcggcgcat cggccggcca ttcggcgaaa tccatgcgcc cggcctccgg
     4381 cagcagccgc ggcagcagca gcgtgcggcc atccctgcgc gcccctgcga ccagcacggc
     4441 cggatccagc tcgccgtcgc atgccagata cacggcgatg ctgcgggccg ccgcataggc
     4501 cggcagcgcc cggatgcgga ccgcgacggc ttcggccgcc gcctgctgcg cggccggcga
     4561 cagggcccgg cgggccgcac gcaggcggcg gcgcagggcg atgacatcga ttgcggaggc
     4621 ggtttcttcg gtcacggcgg ctccggcggc gagggacgac ggccgggcac gctagggcgc
     4681 cgctccgatc cccgcaagcc cccggccgca gacatgaaaa cgccccgctg cgcagccagc
     4741 gcggcggggc gcggaaatcg gggcggatcg gatcgaagcg tccgggatca ggcccgtgac
     4801 cgcggccgac aaggtccgct cagggaccgc ggccgggcat gggtgaagtg catgagctca
     4861 ggcggcgacg tgctgcagag cggtctccag ccatgcgaac cggtcaccgg ggcgcagcgg
     4921 cgccatctgg cgcttgaccg ggcgcaccgt ctcgccgctg gccggattgg cggtcggcag
     4981 catctgcgga cgcgcggccg gggcgttcac cggggacggc aaggtctggg agggctgtga
     5041 agtctgggat gtgcgggtcg actgcgtcgt cggcgcgcca tcctgcgcag gttcggtggc
     5101 ttcggcgctg tagttgttca gggcgatgcc cagcagggcg gcgatcaggg cggcggagct
     5161 ggtggtcatg gcctcaacct cgtggtccgt ggtcgtggtg ccggagcggc gtggcgtgca
     5221 tggccatgac ttgtcgaaat gcgtgccaat ccagattttt atattttcat tcaatgattt
     5281 gaacaaaatc tgccgatcag gccgctgccc ctcgctccga tcggccttcc ggaaaccccg
     5341 cagcgcggcc gaatccgtcg ggcatcagcg acaggtgcgc cggcggtttc acaagtcctt
     5401 gattccagtc gactgacagc cgtcgctgtc gaacgacgtg catgaaaaaa gccggcggac
     5461 gcagccgccg gcttcatcgg acgctcgcga ccgccggact tgccggcggc cggcgggcag
     5521 gctcagaaca tgtgctggcc gccgttgatc gagatgttgg cgccggtcag gaacgccgct
     5581 tcctcggagc acagatacgc gaccagaccc gcgacctcgt tcggctcgcc gaggcggccc
     5641 atcgggatca gcggcaggat cttggtgtcg agcacttcct gcgggatggc gcggaccatc
     5701 ttggtgccga tgtagcccgg cgagatggtg ttgacggtca cgcccttctt gcagacctcg
     5761 agcgccagtg ccttcgtgaa gccatgcatg ccggccttgg ccgctgcgta gttggtctgg
     5821 ccgaaggcgc ccttgatgcc gttgaccgac gagatgttga cgacgcgccc ccagttgcga
     5881 gccatcatgc cgtccatgac ctgcttggtc atgttgaaca cggaatccag gttggtcttg
     5941 atgaccgcgt cccagtcggc cttggtcatc ttcttgaagg tcatgtcgcg ggtgatgccg
     6001 gcgttgttga ccagcacgtc gatcggaccg atgtcggcct cgacctgctt gacgcaggcc
     6061 gcgcaggagt cgaagtcggt gacatcgacc ggcactgccg agaagtcgaa gccctgcgcc
     6121 ttcatgcccg cgagccattc ctcggcggtc ttgttgcccg gcgaataggt cgccacgacc
     6181 ttgtccccca gcgccgcgag cttgatgcag atcgcctcgc cgagtccccc catgccgccg
     6241 gtcactacag ccactcgtgc cattgcaaaa cctcctcgtt cgaattcagc acatgaaccc
     6301 ggccgctcct ttgcgggcac gaacgtgacc tgtgaggcag gcgaacgccc cgtgcggatc
     6361 ggcgggccgg tttttgtggt gcttctttaa aagtgtttcc tgtcgcgttt cgggcatgcg
     6421 gcagcggttc ctgccggcat gactgctttg cacgcgtgca ccgctcgtga cggtaccgcc
     6481 cccctgctgt tatgcgaccc ggctagtatg ttctcgcccg gacgggatgc caagccacgc
     6541 ggcgcacccg attgaccgga atcaagcgct tgatgagaaa aggatttcta ctcatgcgcc
     6601 acgtgaaatg aactattctt catttcttat aacgggattt gaaatcatca tgatttcact
     6661 tgatgacatc atcaaggctc gccggcgcat ccgcgacagc ctgccgccga cgccgtgcgc
     6721 ccgcgccgcg cagctgtcgc gccagctcgg ctgcgagctg ttcatcaagt gcgaaaacct
     6781 gcagatgacc ggctcgttca aggtgcgcgg cgcgctcgcg cgcatcctgc agctgaccga
     6841 ggccgagcgc gccgccggcg tcatcgccgc ctcggccggc aaccacgcgc agggcgtggc
     6901 ctgggcggcg cagaaattcg gcgtgccggc gaccatcgtc atgccggaaa ccgcgccgct
     6961 tgcgaagatc cgcggcactg ccgccttcgg ggccgacatc gtgctcgccg gcaccggcta
     7021 cgacgccgcc tatgcgcgcg ccgtcgaact gcagcgcgac agcggcgcga ccttcatcca
     7081 cgccttcgac gatccggcgg tgatggccgg ccagggttcg gcggggctgg agatcatcga
     7141 gcagctcggc gatttcgacc tggtgctggt gccggtcggc ggcggcggcc tcgccggcgg
     7201 catcgccacc gtcatcaagc agacccggcc cggcgtgcag gtgatcggcg tgcagagcgc
     7261 gcgcgtgccg tcgatgcagg cgagtctcgc cgccggcgcg ccgacgccgg tgccgccggc
     7321 ggcgacgctc gccgacggca tcgcggtcgc gcgtgtcggc gtgcacacct atccgcaggc
     7381 cgcggcctgg ctcgacggca tcgtcaccgt cgacgaggaa tcgatcgcgc gcgcgatcct
     7441 gaccctgctc gaacaggaaa agctgctggc cgaaggcgcc ggcgcggtcg gcatcgcggc
     7501 gctgctcgat ggcgtcgtcg gcgacccggc cggcaagcgc atcgtcgccg tggtctcggg
     7561 cggcaacatc gacccgatcg aactcgcgaa gatcgtcgag cgcgggctgg aacgcgacgg
     7621 gcggctcgcg cacctgcgcg tcgtcgtcgc cgaccggccc ggctcgatcg ccgaggtcgc
     7681 cggcctggtc gcgcgcgagc gcgccaacat cctgcagatc agccagcgcc gtgacgccgc
     7741 cgaggtcggc ctgcgcgagg ccgaactcga cctgacgctg gaaacccgcg ggctcgatca
     7801 cgtcaccggc atcgtgcagg cgctgcgcga cggcggtttc cgcgtacgct gacgaaccgc
     7861 cgccgttcgc gcgggtccag attccgtcgc cccgcctcat ccgccggtat cccgagatgt
     7921 ccgattccgt ccccgcgccg ctgaccgctg ccgaggtccg cgccgtcgtg ctcggctcgg
     7981 tgctggcgct gttcctcgcc gcgcttgacc agagcatcgt cgccacggcg ctgccggcga
     8041 tcgccgccga gctcggccgg gtcgagctga tgtcctgggt ggtcaccgcc tacctgctcg
     8101 cctcgacctg cgccaccccg gtgctcggca aggcgagcga cgtgctcggg cgccggcgcg
     8161 tgctcgccgg ctgcctgatc gtgttcctcg gcggctcggt gctctgctcg ctcgcgactt
     8221 cgatgccgat gctgatcgcg gcgcgcgcgc tgcagggcct cggcggcggc gggctgatca
     8281 cgctggcgca ggcgacggtc gccgacgtgg tgccgccgcg cgagcgcggg cgctacgtcg
     8341 cctacttcgc gtcggtctgg gcctgcgccg gcctgctcgg accgacgctc ggcgggctgc
     8401 tgacgcaata cctgggctgg ccgtggatct tctggatcaa cctgccgctc ggactcgcgg
     8461 cgctcgtgat caccgaccgc gtgctgcgcc ggctgccggt gcgcggcggc acgcgcgggc
     8521 gcatcgacct gcccggcatc gtgctgctgt cgacggcgac gacggcgctg ctgctggcgc
     8581 tgagcttcgg cggccaccgc tggccgtggg cctcggcgcc gatcctcggc ctgctggcgc
     8641 tcgccgtcgt tccggcgctg ctgttcgcgc accgccagcg gcacacggcc gagccgctgc
     8701 tgccgccggc cttcttccgc gatccggtgg tcgcaccgac cggcgcggcg ctgttcctgg
     8761 tgttcggcgg cttcctcgcg gtcgcggtgc tggtgccggc ctggctgcag atcggcctcg
     8821 gcaggagcgc cgccgcggcc gggctgctga tgatgccgat gtcactggcc gcgccgctga
     8881 ccgcgacgct gaccggccac tacctgcgcg cgaccggccg ctaccgccgg ccgccgcagc
     8941 tcggcctgcc gctggcggcg gtggcac
//
LOCUS       DAOQIE010000152         8962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3950_length_8962_cov_7.726732, whole genome shotgun sequence.
ACCESSION   DAOQIE010000152 DAOQIE010000000
VERSION     DAOQIE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3950_length_8962_cov_7.726732"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PK144_10475"
     CDS             complement(<1..792)
                     /locus_tag="PK144_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNF66196.1"
                     /translation="MSVPARVFISYSHEPAAQRPRVEALAAALKDHGIGVVIDHDYDT
                     TCTHWPRWCMEQTAKGVTDFILCICTDEYSRRIVGDVPADVGKGVYWEGSLFIAELYD
                     DKNNMRLIPVLLDGEPAASIPRFLAGWTQCPIRAFTPDDAGFALLLGIVRRTAPLCRA
                     ALNDLDYQSRVEVSCPPDVACGHIPAHGPRNGFIGRHAELAALDAAWADTGRTRLLTL
                     IAPGGTGKTALVLHWLAGLRDAGWDEAQRVFGWSFYSQGTAEDRQA"
     gene            complement(857..1804)
                     /locus_tag="PK144_10480"
     CDS             complement(857..1804)
                     /locus_tag="PK144_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNF66197.1"
                     /translation="MSAPAPVTPEQRERLLRLATRASVATALLLIVGKTVAWLMTGSV
                     SVLASLVDSVMDALASLVNLVAVRVSLAPPDAEHRFGHGKAEALAGLGQATFIAGSAL
                     FLCLQAVDRLLRPQPLTDIGVGAAVIVFAIVATAVLLAIQRHVIRRTGSTAIRADALH
                     YLTDLATNASILLALGLGHFGYDGLDPLFALGIAVYILKSAWEIGAEAVSLLMDHELP
                     AETKQRIVELAREQHGVLGVHDLRTRQSGHTIFVQLHLELADSLPLAVAHAIGDAVES
                     AIENEFPGAEILIHQDPVSAVAQERRRMLAHQMGERAGR"
     gene            1942..2109
                     /locus_tag="PK144_10485"
     CDS             1942..2109
                     /locus_tag="PK144_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66198.1"
                     /translation="MSEKHRPSIPNRTAQAEAWEALTLFARQAQRQPAANAQAARCRP
                     PVSHPQQSGKD"
     gene            2298..2471
                     /locus_tag="PK144_10490"
     CDS             2298..2471
                     /locus_tag="PK144_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66199.1"
                     /translation="MDTDCPARPAETRESPESTTPAQPILVPRWLRVAMRPQPDSDNG
                     HRSGNRWQAARKH"
     gene            complement(2532..3479)
                     /locus_tag="PK144_10495"
     CDS             complement(2532..3479)
                     /locus_tag="PK144_10495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF66200.1"
                     /translation="MRRVPLAGFDGLPLLAECFAASAPASAVLLLVHGAGECPVRYVE
                     LIADWRRRHPDHAICAVTHRGHGAAGRVPPLCGDDPVERQKMHVDHFDDYARDLDIYA
                     TLARSLAPGRPLYALAHSMGAAILLRWLQGGERGVAAAAITAPMVEIRGLWGLPVSRD
                     ALGRLALATVGRQRPSAWAANQGPYHEPLFRDARGLNGLTASERRFAMRARLIERHPY
                     SRIGGPTWGWVAAAVAAGRRIFAEVGRLAGVPMLCVVPGIDRLVWPPSGLRLAARIRA
                     TGGDCTLLSLPEARHEVLIETDAIRTQVLEAVEARFAGP"
     gene            complement(3593..4576)
                     /locus_tag="PK144_10500"
     CDS             complement(3593..4576)
                     /locus_tag="PK144_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNF66201.1"
                     /translation="MSISPDDPEPMYRIGTVSRLTGIATVTLRMWERRYNVVDPGRSS
                     GRNRLYSRDDIARLALIKRLVDAGHSISTVAGLTLVQLHERLEVQEKPRQLAALPVRV
                     LTPVRVAVLGDALPARIARAGSELAGLAIVASQRERARFLADAPAQEPQVLVIEVPAL
                     GPETAAGVEELRQACGARHVVIVYGFGRRATVAKLDTAYSTPLRAPVGMAELRIACRA
                     GLGVAALPLPERQPVDLTPSPDGIPQRRYDTDTLERIAASTPTLACECPHHLVDLVAG
                     LSAFEDYSAQCENRSPEDAALHHWLRATTAQARALMEEALTRVAEAEGLLR"
     gene            complement(4617..5714)
                     /locus_tag="PK144_10505"
     CDS             complement(4617..5714)
                     /locus_tag="PK144_10505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNF66202.1"
                     /translation="MKAVLALDPLLPLRSAAAGVGQRFDALAIELAERGWLPDAVLRA
                     GIRRLLAARLDSEARRVAQRTAWIESLHAGPIALATDAANRQHYEVPAAFFEHVMGRH
                     LKYSCGFWPEGVHTLDEAEAAMLALTCERAALADGQDILELGCGWGSLTLWMAERYPH
                     ARITAVSNSRTQRRYIQSRLRARGLRNVQLITADMNDFASGPSLNGAFDRVVSVEMFE
                     HMHNWPALLACVKGWLRAEGRLFLHVFCHRRYSYPFVDEGSDDWMARHFFTGGLMPGA
                     DLPMRFQDDLQLARHWWIDGRHYQATCEAWLAGQDRRRDEILRLFAADGDPAEAPRQF
                     RRWRMFFLACSELFGYREGSEWGVGHYLYTR"
     gene            complement(5862..6611)
                     /locus_tag="PK144_10510"
     CDS             complement(5862..6611)
                     /locus_tag="PK144_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNF66203.1"
                     /translation="MTLAWAHQQRTHDATAVDVCWAAGMGGLAIAAALLAGGDPARRT
                     LVALLGGLWSARLAGFLYRNRVRGHAGEDARYRRLRAAFGRHAGAGFLAFYQGQALAT
                     ALFALPFLACAQAQAPLSGPAAIAALLVWALAVGGESLADRQLARWRAEPAHRGRTCR
                     TGLWAWSRHPNYFFEWLHWWTYPLLALGSEAFWPTLAGPLLMYATLHYATGIPHTERQ
                     ALAHRGADYAAYQREVPEFFPRPPRPPERGA"
     gene            complement(6673..7263)
                     /locus_tag="PK144_10515"
     CDS             complement(6673..7263)
                     /locus_tag="PK144_10515"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HNF66204.1"
                     /translation="MSRTGRWLLVNLAAFQIVWWSAVLGAAHGLPWAGPLTALGACAL
                     HVRRIAPETRSAELTMLAYALCFGLACDATLMAFGLIRYAPGLGIGPDWLAPAWMAAL
                     WPTFATTLNHSLGWLRRHPLCAALFGALAGPLAYGGGVVLGAAGWPRGALAGMTAIAA
                     LWAVTLPLLSTLSAVTVIPAEPEPSPEPVRPLAGSL"
     gene            complement(7260..8522)
                     /locus_tag="PK144_10520"
     CDS             complement(7260..8522)
                     /locus_tag="PK144_10520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNF66205.1"
                     /translation="MNEQTLPAAPAAAAGAGDTADRLLRRVVFQRLRGLARGRLILVD
                     ADGQHSFGPGGHGPQVRVEILDPGVWARIALRGTVGVGEAYVDGGWRADDLTALVRLF
                     VLNKPVMDGMERGLARLALPLLKLWHWLHPNTRHGSRANIAAHYDLGNAFYQTFLDET
                     LMYSCALFEHPGMSLAEASQAKNERICRKLGLRAGDHVLEIGTGWGGFALHAAGRHGC
                     RVTTTTISREQYELARERVAAAGLGNRVTVLFEDYRELAGRYDQLVSIEMIEAVGHRW
                     FDTYFRTCSRLLAPDGQMLLQAITIRDQEYARTKRSVDFIQRHVFPGGCLPSVQALAD
                     TVARVTDLRALHLEDIGPHYATTLRHWRERFLANLDRVRALGFDDTFLRLWEYYLAYC
                     EGGFAERYLGTVQLLLAKPECRRAVLPS"
     gene            complement(8519..>8962)
                     /locus_tag="PK144_10525"
     CDS             complement(8519..>8962)
                     /locus_tag="PK144_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HNF66206.1"
                     /translation="LAEVTNLPWGERHCYVLDLHDQPPAARVPFAKRLHVSPFLPMAL
                     DYLWHGYAPGERLGVHLEVFARDRQPGAAGAPLFDATLHLARRPLDAAQAALALVRYP
                     LMSLQIVAAIHWQALRLWLKRVPVFERPVPPVPPVSTTCPGETDR"
BASE COUNT         1393 a         3167 c         3275 g         1127 t
ORIGIN      
        1 cgcctgccgg tcctcggccg tgccctggct gtagaagctc cagccgaaca cgcgctgcgc
       61 ctcgtcccag ccggcgtcgc gcagccccgc cagccagtgc agcaccagcg ccgtcttgcc
      121 ggtgccgccg ggggcgatca gcgtcagcag gcgggtgcgg ccggtgtcgg cccaggcggc
      181 atcgagcgcg gccagttcgg cgtggcgacc gatgaagcca ttgcgcgggc catgcgccgg
      241 aatatgtccg caggccacgt ccggcgggca ggacacctcc acgcgcgact ggtaatccag
      301 atcgttgagg gccgcccggc agagcggagc cgtgcgccgg acgatgccga gcaacagcgc
      361 gaaacccgca tcgtccggcg tgaatgcccg gatcgggcac tgggtccatc cagcaagaaa
      421 gcgcggaatg ctggcggcgg gctcgccatc gagcagaacc gggatcaggc gcatgttgtt
      481 cttgtcgtca tacagctcgg cgatgaacag cgagccttcc caatacacgc ctttgccgac
      541 atcggccggc acatctccga cgatgcgccg gctgtattcg tccgtgcaga tgcacaggat
      601 gaaatcggtc acgcccttcg ccgtctgctc catgcaccag cgcggccagt gggtacaggt
      661 cgtgtcgtag tcgtgatcga tgacgacgcc gatgccgtga tccttcaggg ctgcagccag
      721 cgcctcgacc cgcggccgtt gcgctgcggg ctcgtggctg tagctgatga acacccgcgc
      781 gggcacggac atcgcggact cccttccggt cggggaatgc ccggcgagcg tagccgaagc
      841 agcgcgcgcg gaacgctcag cgcccggcgc gttcgcccat ctgatgcgcg agcatgcgcc
      901 ggcgctcctg cgcgacggcg ctgaccgggt cctgatggat caggatttcg gcgccgggga
      961 attcgttttc gatcgccgat tcgacggcat cgccgatcgc gtgcgcgacc gcgagcggca
     1021 gcgagtcggc gagttcgagg tgcagctgca cgaagatcgt gtgcccggac tggcgcgtgc
     1081 gcaggtcatg cacgccgagc acgccgtgct gctcgcgcgc cagctcgacg atgcgctgct
     1141 tggtttcggc cggcagctcg tgatccatca gcaggctgac ggcctcggcg ccgatctccc
     1201 acgcgctctt gaggatgtag acggcgatgc cgagcgcgaa cagcgggtcg agcccgtcgt
     1261 agccgaaatg accgagcccg agcgcgagca ggatgctggc gttggtggcg aggtcggtca
     1321 ggtagtgcag cgcatcggcg cggatcgccg tcgagccggt gcggcggatg acgtggcgct
     1381 ggatcgccag cagcacggcg gtcgcgacga tcgcgaacac gatcaccgcc gcgccgacgc
     1441 cgatgtcggt cagcggctgc gggcgcagca ggcgatcgac cgcctgcagg cacaggaaca
     1501 gcgccgaacc ggcgatgaag gtcgcctgcc cgagcccggc cagcgcctcg gccttgccgt
     1561 ggccgaagcg gtgctcggca tcgggcggcg ccagcgacac gcgcaccgcg accagattca
     1621 ccagcgaggc cagcgcatcc atcaccgaat ccaccagcga ggccagcacc gacaccgagc
     1681 cggtcatcag ccacgccacc gtcttgccga cgatcagcag cagcgccgtc gcaacggaag
     1741 cacgggtcgc gagccgcagc aggcgttcgc gctgctcggg agtgacgggg gcgggggcag
     1801 acattgcgga ctccggtcgc gggcgggcgg cgcgaattgt acggggggaa ccttgcaatg
     1861 cagcaaggtg gcaccggccg cgacggatcg tcatggacga tcggcagagt agcggcatcc
     1921 accgagcggg gaaccggagc catgtccgaa aagcaccgtc cgagcatccc gaaccgcacc
     1981 gcgcaggccg aagcctggga ggcgctgacg ctgttcgccc gtcaggcgca acgccagccc
     2041 gcagcgaatg cgcaggctgc acgttgcaga ccgcctgttt cacatccgca acaatccggc
     2101 aaggactgaa acgcgaaaca gttcgcggct ctgcacgaca caaagtgcgg atttacagga
     2161 agttaatcaa aatgcaacgc gagttttcaa acgacaggca gattacggcg ggcgccggat
     2221 aggcgccgcc gcaacagcgg cgcatgctga gggccatccg atcctccccc cgcccgcatc
     2281 gcccgaggca tacccagatg gacaccgact gcccggcccg cccggccgaa acccgcgaat
     2341 ccccggaatc caccactccg gcccagccga tcctcgtgcc ccgctggctg cgcgtggcca
     2401 tgcgcccgca gccggacagc gacaacggcc accgcagcgg caaccgctgg caggcggcgc
     2461 gcaagcactg atccgcccgc ggtcttcctg aatttccgca acgacgtcgg ctccgaccgg
     2521 cgtcggtcgt ttcagggccc ggcaaagcgc gcttccaccg cctccagcac ctgcgtgcgg
     2581 atcgcgtcgg tttcgatcag cacctcgtgg cgggcttcgg gcagcgacag cagcgtgcag
     2641 tcgccgccgg tcgcgcggat gcgtgcggcc agacgcagac ccgaaggcgg ccagaccaga
     2701 cggtcgatgc cgggcacgac gcagagcatc ggcacgccgg cgagccgccc gacctcggcg
     2761 aagatgcgcc ggccggcggc gaccgccgcc gccacccagc cccaggtcgg gccgccgatg
     2821 cgcgaatacg ggtgacgctc gatcagccgc gcgcgcatcg cgaagcgccg ttcgctggcc
     2881 gtgaggccgt tgagcccgcg ggcatcgcga aacagcggtt cgtggtacgg cccctgattc
     2941 gccgcccagg cgctcggacg ctggcgcccg acggtggcca gcgccagccg gccgagtgcg
     3001 tcgcggctga ccggcaggcc ccagaggccg cggatctcga ccatcggcgc cgtgatcgcc
     3061 gccgccgcca cgccgcgctc gccgccctgc agccagcgca gcaggatcgc cgcccccatc
     3121 gaatgcgcga gcgcgtacag cggccggccc ggcgccaggc tgcgcgcgag cgtcgcgtag
     3181 atgtccagat cgcgcgcgta gtcgtcgaaa tgatcgacgt gcatcttctg acgctcgacc
     3241 ggatcgtcgc cgcagagcgg cggcacgcgg ccggcagcac cgtggccgcg gtgcgtgacc
     3301 gcgcagatgg cgtggtccgg atggcggcgg cgccagtcgg cgatcagctc gacgtagcgc
     3361 accgggcatt cgccggcccc gtgcacgagc agcagcacgg cgctggccgg ggcgctcgcg
     3421 gcgaagcatt cggcgagcag cggcaggccg tcgaaaccgg cgagcggcac gcgccgcacg
     3481 gcggcctcgt gcgcggcaag cagcgcatcg agccgttcgc agtccggacc ggcggcttgc
     3541 gccagcgcgg catccgatgc ccatgtggcc gcggcggccc ggtcggcggc gctcagcgca
     3601 gcagcccttc ggcctcggcg acgcgggtca gggcctcttc catcagcgcc cgcgcctgcg
     3661 cggtggtcgc gcgcagccag tgatgcagcg ccgcgtcctc ggggctgcgg ttctcgcact
     3721 gcgcgctgta gtcctcgaag gccgacagcc cggccacgag atcgacgagg tgatgcgggc
     3781 attcgcaggc gagcgtcggc gtcgaggcgg cgatgcgctc cagcgtgtcg gtgtcgtagc
     3841 gccgctgcgg gatgccgtcc ggcgacggcg tcaggtcgac cggctggcgc tcgggcagcg
     3901 gcagcgccgc gacgccgagc ccggcgcggc aggcgatgcg cagctccgcc atgccgaccg
     3961 gcgcgcgcag cggcgtgctg taggcggtgt cgagcttcgc gaccgtcgcg cgccggccga
     4021 agccgtagac gatgaccacg tgccgcgcgc cgcaggcctg gcgcagttcc tcgaccccgg
     4081 cagccgtctc cggcccgagc gccggcacct cgatgaccag cacctgcggc tcctgcgccg
     4141 gggcatcggc gaggaagcgc gcgcgctcgc gctggctggc gacgatggca aggcccgcca
     4201 gttcgctgcc ggcgcgggcg atgcgcgccg gcagcgcatc gccgagcacg gccacgcgca
     4261 ccggcgtcag cacccgcacc ggcagtgccg cgagctgccg gggcttctcc tggacttcga
     4321 ggcgctcgtg cagctgcacg agcgtcaggc cggcgacggt gctgatcgag tgtccggcat
     4381 cgaccagacg cttgatcagc gccagccggg cgatgtcgtc gcgcgagtac agccggttgc
     4441 gaccgctgga gcgaccggga tcgacaacgt tgtagcgccg ctcccacatg cgcagcgtga
     4501 ccgtggcgat gccggtcagg cgggagacgg tgccaatgcg gtacatcggt tcgggatcgt
     4561 cgggtgaaat gctcatgacg gcttgtctcg gatgccggac atccatggcc gcaggctcag
     4621 cgggtgtaca ggtagtgacc gacaccccat tcactgccct cccggtaacc gaacagttcg
     4681 ctgcacgcca gaaagaacat gcgccagcgc cggaactgcc ggggggcttc ggccggatca
     4741 ccgtcggcgg caaacagccg caggatttcg tcgcgccggc ggtcctggcc ggcgagccag
     4801 gcctcgcagg tcgcctggta gtgacgcccg tcgatccacc agtgacgggc cagttgcagg
     4861 tcgtcctgga agcgcatcgg cagatccgcg cccggcatca ggccgccggt gaagaaatgt
     4921 cgtgccatcc agtcgtcact gccttcgtcc acgaacggat aactgtagcg tcgatggcaa
     4981 aagacgtgca ggaacaatcg cccttcggcg cgcagccagc ccttcacgca cgcgagcagc
     5041 gccggccagt tgtgcatgtg ctcgaacatc tcgaccgaca cgacgcgatc gaacgccccg
     5101 ttcagggagg gcccgctggc gaagtcgttc atgtcggccg tgatgagctg cacgttgcgc
     5161 aggcctcttg cacgcaggcg cgactggatg tagcgccgct gcgtgcgcga gttcgacacc
     5221 gccgtgatgc gtgcgtgcgg gtagcgctcg gccatccaca gcgtcagcga gccccagccg
     5281 cagccgagtt cgaggatgtc ctgaccatcg gcgagcgcgg cgcgctcgca ggtcagggcc
     5341 agcatcgccg cctcggcctc gtcgagcgtg tgcacgccct cgggccagaa gccgcaggag
     5401 tacttcaggt gccggcccat cacgtgttcg aagaaggccg ccggcacctc gtagtgctgg
     5461 cggttggcgg cgtcggtcgc gagcgcgatc gggccggcgt gcagggattc gatccaggcc
     5521 gtgcgctgcg cgacgcggcg cgcctcgctg tcgagccgcg cggcgagcag ccggcggatg
     5581 ccggcgcgca gcacggcatc gggcagccag ccgcgctcgg ccagttcgat cgcgagggca
     5641 tcgaaacgct ggccgacacc ggccgcggca ctgcgcagcg gcagcagggg atcgagggcg
     5701 aggacggctt tcatcggaac atctcctgcg ggcacggacg gggacggggc gacggtcgcc
     5761 gggccggtca cgcagcagcg cctcgctttc cagccgcctc gccccccatg gaagaggcgc
     5821 cgtggagcgg cggaacccgg ggcatctgcg cgtgaccggt ctcaggcacc tctttcggga
     5881 gggcgcggcg gacgggggaa gaattccggc acctcgcgct ggtaggcggc gtaatcggcg
     5941 ccgcggtgcg cgagcgcctg gcgttcggtg tgcgggatgc cggtggcgta gtgcagcgtc
     6001 gcgtacatca gcagcggtcc ggccagcgtc ggccagaagg cctcgctgcc gagcgcgagc
     6061 agcggatagg tccaccagtg cagccattcg aagaagtagt tcgggtgtcg cgaccaggcc
     6121 cacagcccgg tgcggcaggt ccggccccgg tgcgccggct cggcccgcca gcgcgcgagc
     6181 tgccggtcgg ccagggattc gccgccgacc gcgagcgccc agaccagcag cgcggcaatg
     6241 gcagccggcc cggacagcgg cgcctgcgcc tgcgcgcagg cgaggaaggg cagcgcgaac
     6301 agcgccgtgg ccagcgcctg cccctgatag aaggccagga agccggcgcc ggcgtgtctg
     6361 ccgaaggccg cgcgcaaccg ccggtagcgg gcatcctcgc cggcgtggcc gcgcacgcgg
     6421 ttccggtaca ggaaaccggc aagacgcgcc gaccacaggc cgccgagcag cgcgaccagc
     6481 gtccggcgcg ccggatcacc gccggcgagc agcgccgccg cgatcgcgag gccgcccatg
     6541 ccggcggccc agcagacatc gacggccgtg gcatcgtgcg tgcgctgctg gtgcgcccac
     6601 gcgagcgtca tcaccagcgc cgcggccgcc cagccgccga ggaacagggc cgtgacgctc
     6661 accggccatg cctcagagcg atccggccag cgggcgcacc ggttccggcg atggctccgg
     6721 ctcggccgga atcaccgtca cggccgagag cgtgctcagc agcggcagcg tcacggccca
     6781 gagcgccgcg atcgccgtca ttccggccag cgcaccgcgc ggccagccgg cggccccgag
     6841 cacgacgccg ccgccgtagg cgagcgggcc ggccagcgcg ccgaagagcg ccgcgcacag
     6901 cggatggcgg cgcagccagc cgaggctgtg gttcagcgtc gtcgcgaacg tcggccacag
     6961 cgccgccatc cacgccggcg ccagccagtc cggcccgatg ccgagcccgg gcgcgtagcg
     7021 gatcaggccg aaggccatca gcgtcgcgtc gcaggcaaga ccgaagcaga gcgcgtaggc
     7081 gagcatcgtc agctccgccg agcgcgtctc cggcgcgatg cgccgcacgt gcagggcgca
     7141 ggctcccagc gccgtcagcg gtccggccca gggcaggccg tgcgcggcgc cgagcacggc
     7201 cgaccaccag acgatctgga aggcggcgag attcacgagc agccagcggc ccgtgcggct
     7261 catgacggca gcaccgcgcg ccggcactcg ggcttggcca gcagcagctg cacggtgccg
     7321 agataacgct cggcgaagcc gccctcgcag taggcgaggt agtactccca gaggcgcagg
     7381 aaggtgtcgt cgaagccgag cgcgcgcacg cggtcgaggt tggcgaggaa gcgctcgcgc
     7441 cagtggcgca gcgtcgtcgc gtagtgcggg ccgatgtctt cgaggtgcag cgcgcgcagg
     7501 tcggtcacgc gcgcgaccgt gtcggccagc gcctgcaccg agggcaggca gccgcccggg
     7561 aagacatggc gctggatgaa gtcgacgctg cgcttcgtgc gcgcgtactc ctggtcgcgg
     7621 atcgtgatcg cctgcagcag catctggccg tccggcgcca gcagccggct gcaggtgcgg
     7681 aagtaggtgt cgaaccagcg gtggccgacc gcctcgatca tctcgatcga caccagctgg
     7741 tcgtagcggc cggcgagttc gcggtaatcc tcgaacagca ccgtgacccg gttgccgagc
     7801 ccggcggcag cgacgcgctc gcgcgcgagt tcgtactgct cgcgcgagat cgtcgtcgtc
     7861 gtcacgcggc agccgtggcg cccggctgcg tgcagcgcga agccgcccca gccagtgccg
     7921 atctccagca cgtggtcgcc ggcacgcagg ccgagcttgc ggcagatgcg ctcgttcttc
     7981 gcctgcgagg cttcggcgag gctcatgccg gggtgctcga acagcgcgca ggaatacatc
     8041 agcgtctcgt cgaggaaggt ctggtagaag gcgttgccga ggtcgtagtg cgcggcgatg
     8101 ttggcgcggc tgccgtggcg ggtgttcgga tgcagccagt gccagagctt cagcagcggc
     8161 agcgccaggc gcgccagccc ccgttccatg ccgtccatga ccggcttgtt cagcacgaac
     8221 agccgcacca gcgccgtcag gtcatcggcc cgccagccgc cgtcgacgta ggcctcgccg
     8281 acgccgaccg tgccgcgcag cgcgatgcgc gcccagacgc cggggtcgag gatctcgacg
     8341 cgcacctgcg gcccgtgccc gccggggccg aagctgtgct ggccgtcggc atcgacgagg
     8401 atcagccgcc cgcgcgcgag cccgcgcagg cgctggaaca ccacgcggcg caacagccgg
     8461 tcggcggtat cgccggcacc ggcagcggcg gccggtgcgg cagggagcgt ctgttcgttc
     8521 atcggtcggt ctccccgggg caggtggtgg aaacaggcgg cacgggcggc acgggccgct
     8581 cgaacaccgg cacgcgcttg agccacagcc gcagcgcctg ccagtggatc gcggcgacga
     8641 tctgcaggct catcagcggg tagcgcacga gcgcgagtgc ggcctgcgcg gcatcgagcg
     8701 ggcggcgggc gaggtgcagc gtcgcgtcga acagcggtgc gccggcggcg ccgggctggc
     8761 ggtcgcgcgc gaagacttcg aggtgcacgc cgaggcgttc gccgggcgcg tagccgtgcc
     8821 acaggtagtc gagcgccatc ggcaggaacg gcgacacgtg caggcgcttg gcgaagggca
     8881 cgcgcgcggc cggcggctgg tcgtgcaggt cgagcacgta gcagtgacgc tcgccccagg
     8941 gcaggttcgt gacctcggcg ag
//
LOCUS       DAOQIE010000153         8890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_3988_length_8890_cov_7.690436, whole genome shotgun sequence.
ACCESSION   DAOQIE010000153 DAOQIE010000000
VERSION     DAOQIE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_8890_cov_7.690436"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..1198)
                     /locus_tag="PK144_10530"
     CDS             complement(296..1198)
                     /locus_tag="PK144_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66207.1"
                     /translation="MNPDPESGTRLRAGLAAALLTLALPAAAADLADAITTAAAALQP
                     LAGDALAVSVCTLTLAGRAMPLLQLTERAADGKPQRRSLLLDDALALRGRLDHGSGAV
                     QRCSGDRLVIRGSVEIDGRRLDGPVLRITADAQVQADATDPNQLAIAGREPPASGRPA
                     AVVQAARAQGFDALRRAAVAPRGQTLAAWQPVCVLEAGGVRVPVLGWLALTLPKAAAG
                     DYVPPARGSSGLLLTDASGAPLAQLDVPPGAVTACHGAVLQLAQPLYGGDCKARGWRC
                     SGEEGDVVELLPGRREFGLGRSGR"
     gene            complement(1284..2057)
                     /locus_tag="PK144_10535"
     CDS             complement(1284..2057)
                     /locus_tag="PK144_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNF66208.1"
                     /translation="MALTQGFKDTMTAAVADPAAADYDTALKAEIDAYAARFKGTPDF
                     PDPLDWRLFKAQLLIESGGPQGKVWKGRVMQIGNKGDPGLSVVKAAAEGRIADAEGAD
                     LVLSADLKSAMKDPKQSTDQPVLNLRIGLCYLFARLIKNIRDEDIIDTSRPPETWKVL
                     KGDSWWKIARHFRIKVKDLQDLNSSAVTRGLFPGMELHVRWGKHVRELTGWRTFDYAT
                     IADRYNGGGDADYADKLEFVHKLLLAAPKPAPTPGAPKK"
     gene            complement(2179..2493)
                     /locus_tag="PK144_10540"
     CDS             complement(2179..2493)
                     /locus_tag="PK144_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNF66209.1"
                     /translation="MDERLKTNLRRQSFWQRAAYMLVFAVILGFAGSLAALLALFQLG
                     ALLLTGEPNARLRPVADGLTRYLLQLWRYQTFLTETPPFPFAEWPVSDAGAREHIVLP
                     HG"
     gene            complement(2837..3451)
                     /locus_tag="PK144_10545"
     CDS             complement(2837..3451)
                     /locus_tag="PK144_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66210.1"
                     /translation="MVRIVPPVRPAPREWNIMRNAHSALYSAVLAGILAIGIGSAAQA
                     AMSEAQYDAAVKAADARLKTDKERCSTMSGNAEDVCKAQAEAEHTRVTEQAKAEMKGT
                     SKARLEAAEEVAEANYKVAKERCDDFSGDPKDICLKEAKATYEKQKADITVLEKGNTA
                     DARKDARETKRDADFEVAKAKCDQFSGDVKDKCVKDAKATYKVE"
     gene            complement(3547..3744)
                     /locus_tag="PK144_10550"
     CDS             complement(3547..3744)
                     /locus_tag="PK144_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNF66211.1"
                     /translation="MNEDIIKGKWKQLKGSLKAKWGDLTDDDLDIAEGNSDYLAGRIQ
                     ERYGRTKDEAKREVDEYTRTL"
     gene            4034..5536
                     /locus_tag="PK144_10555"
     CDS             4034..5536
                     /locus_tag="PK144_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNF66212.1"
                     /translation="MSLALAHCRAQLGIDAPPVAVEVHLSNGLPGLAIVGLPEAAVKE
                     SKDRVRGAILNSRHEYPQRRITVNLAPADLPKEGGRFDLPIALGILAASGQLPGEPLA
                     ALECLGELALGGALRGVRGVLPAALAARDAGRALLLPADNADEAALVEGLTVLPARHL
                     LEVCAHLRGDVRLAPHRLDGLPAADPALPDLADVLGQAHARRALEIAAAGAHNLLYCG
                     PPGTGKSMLAARLPGILPPMSEAEAIETAAVASVSTQGFRLQDWRRRPFRAPHHTASG
                     VALVGGGGNPRPGEISLAHHGVLFLDELPEFNPRVLEVLREPMESGHITIARAARSAD
                     YPARFQLIAAMNPCPCGYLGDAERSCSCSPEQVKRYRARVSGPLLDRIDLQLEVPRLP
                     RTALRGETPEEPSAVVRVRVLAARERQLARAGKANARLEPRELTRDCRLGDAEQRLLE
                     AAMERLGLSPRAYHRILKVARTIADLAASEGITTAHLSEAIGYRRLDRGR"
     assembly_gap    5610..5709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5745..6161
                     /locus_tag="PK144_10560"
     CDS             5745..6161
                     /locus_tag="PK144_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNF66213.1"
                     /translation="MTHDPNCLFCRIVRGEIPAVQVFEDEDTIAFMDIFPASEGHALV
                     IPKLHAVNLLEMPDDALTAVHRSARRLAAAMMRGLGPAGFQIRQFNGAEAGQTVFHYH
                     VHVVPCYPGARPGLHGRDQADPAVLAGLAGRIRAAL"
     gene            complement(6221..6517)
                     /locus_tag="PK144_10565"
     CDS             complement(6221..6517)
                     /locus_tag="PK144_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNF66214.1"
                     /translation="MLDPKVIDEFARRIAESVPPGLRTLQQDMEKNFRAAMQAAFARM
                     DLVTREEFDVQTQVLTRTRARLEALERRVADLEARLPADGAESATAPGPAASDL"
     gene            6825..7163
                     /gene="glnK"
                     /locus_tag="PK144_10570"
     CDS             6825..7163
                     /gene="glnK"
                     /locus_tag="PK144_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNF66215.1"
                     /translation="MKLVTAIIKPFKLDDVREALSEIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEIAIDDPLVERVVDAVTKAANTGKIGDGKIFVYDLEQAIRI
                     RTGEAGAAAL"
     gene            7230..8576
                     /locus_tag="PK144_10575"
     CDS             7230..8576
                     /locus_tag="PK144_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016562483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNF66216.1"
                     /translation="MTNNPLIKGLGALGLLLLPALALAQEAAAPAAAPTLDAGNTAWM
                     LTSTALVLFMTIPGLALFYAGMVRSKNVLSVMMQCFAITALVTLLWVVYGYSVAFDTT
                     GMVKGEVNLYSFIGGLGKAFMAGVGRDSLTLTIPESVFATFQMTFAIITPALIVGAFA
                     ERMKFSALLLYIVLWFTLSYLPIAHMVWSGDGALMWDWGVLDFAGGTVVHINAGLAGF
                     IACLVIGRRKGFPVTAMPPHNLGYTLIGASMLWVGWFGFNAGSAVAADGSAGMAMLTT
                     QVATAAAALAWMFAEWIAHGKPSVLGIASGAVAGLVAVTPASGTAGPMGALLLGLISG
                     LVCFLASTKLKRAMGYDDSLDVFGVHAVGGIIGAILTGLFADAAFGGKGLAEGVSIAD
                     QLWIQTKGALVTVVYSSLISFISLKVVDAIVGIRVTEEEETQGLDISLHEERGYIL"
     gene            8730..>8890
                     /locus_tag="PK144_10580"
     CDS             8730..>8890
                     /locus_tag="PK144_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNF66217.1"
                     /translation="MKLVSAIIKPFKLDDVREALAEIGIQGITVTEVKGFGRQKGHTE
                     LYRGAEYVV"
BASE COUNT         1250 a         3052 c         3037 g         1451 t
ORIGIN      
        1 cgcgcccggc gcggaacggg ggtgaaggac ggggacggca atggaaacgg ggaaggggac
       61 tgacgcgaaa tccgcagcgg cctggcgcag tgagagggaa ccgatcggca gcgcatcgag
      121 cagaagtcgg tgggcatcga acgattgatc cgccagccga tcgcgttcag gcattccgga
      181 tgtgctgcgt ctcggccgtc ctctgccact cgcactgtcc gctgtcgcgg ctgcgcctga
      241 cgcacccagc ggtgcagcgt cttagccgtg cagccgatct gtcgtggttc atttctcagc
      301 gccccgaccg tcccagcccg aactcccgcc gccccggcag cagctcgacc acatcgccct
      361 cttccccgct gcagcgccag ccgcgcgcct tgcagtcgcc gccatacagc ggctgcgcga
      421 gctgcagcac ggcgccgtgg caggcagtga cggcgccggg cggcacgtcg agctgcgcca
      481 gaggcgcgcc gctggcgtcg gtcagcagca ggccgctgct gccgcgcgcc ggcggcacgt
      541 aatcgccggc ggcggccttc ggcagcgtca gcgccagcca gccgagcacc ggcacgcgca
      601 cgccgccggc ttcgagcacg cagaccggct gccacgccgc cagcgtctgg cctctgggcg
      661 cgacggcggc gcggcgcagt gcatcgaagc cctgcgcgcg cgcagcctgc acgacagccg
      721 ccggccggcc gctcgccggc ggttcgcggc cggcgatcgc gagctggttc gggtcggtgg
      781 catcggcctg cacctgcgcg tcggccgtga tgcgcagcac cgggccgtcg agcctgcggc
      841 cgtcgatctc gacgctgccg cggatcacca gccgatcgcc gctgcagcgc tgcacggcac
      901 cgctgccgtg atcgagccgg ccgcgcagcg cgagcgcgtc atcgagcagc agcgagcggc
      961 gctgcggttt gccatcggcg gcgcgctccg tgagctgcag cagcggcatc gcccggccgg
     1021 cgagcgtcag cgtgcagacc gacacggcca gcgcatcgcc ggcgagcggc tgcagcgcgg
     1081 cggcggcggt ggtgatggca tcggcgagat cggcggcggc ggccggcagc gccagcgtca
     1141 gcagcgcggc ggcgaggccg gcgcgcagcc gggtgccgga ttccggatcg gggttcaagg
     1201 ccgctcgccg attgcggcag cggggggcgg gaccggaaga acggcgctgc acattcacct
     1261 caggcgtccg ggcaggatcg atttcacttc ttcggcgcgc ccggggtcgg tgccggcttc
     1321 ggcgctgcga gcagcagctt gtgcacgaat tcgagcttgt cggcgtaatc ggcatcgccg
     1381 ccgccgttgt agcgatcggc aatggtcgcg tagtcgaagg tgcgccagcc ggtcagctcg
     1441 cgcacgtgct tgccccagcg cacgtgcaac tccatgcccg ggaacaggcc cctggtgacg
     1501 gcgctgctgt tgaggtcctg caggtccttg accttgatgc ggaagtggcg ggcgatcttc
     1561 caccacgaat cgcccttgag caccttccag gtctcgggcg ggcgcgaggt gtcgatgatg
     1621 tcctcgtcgc ggatgttttt gatcagacgc gcgaacaggt agcagagccc gatgcgcagg
     1681 ttcagcaccg gctggtcggt cgactgcttc gggtccttca tggccgactt gagatcggcc
     1741 gacagcacga ggtcagcccc ttcggcatcg gcgatgcggc cctcggccgc ggccttcacc
     1801 accgacaggc ccggatcgcc cttgttgccg atctgcatga cccggccctt ccagaccttg
     1861 ccctgcgggc cgccgctctc gatcagcagc tgcgccttga acagccgcca gtcgagcggg
     1921 tccgggaagt ccggcgtgcc cttgaagcgg gcggcgtagg cgtcgatctc ggccttcagc
     1981 gccgtgtcgt agtcggcggc ggccgggtcg gcgaccgcgg ccgtcatcgt gtccttgaag
     2041 ccctgcgtca gcgccatcgc ggccccccgc gcttcaacgc caccggagat cggtgcaatg
     2101 ccggcgaagt cccggcggaa tgcgaccggg ccgcggcacg cgaggtgcgc ggcggcccgg
     2161 ccggaacttg cacccgcatc agccgtgcgg cagcacgatg tgctcgcgcg cgccggcgtc
     2221 gctgaccggc cactccgcga acgggaacgg cggcgtctcg gtcaggaagg tctggtagcg
     2281 ccagagctgg agcaggtagc gcgtcaggcc gtcggcgacc gggcgcagcc gcgcgttcgg
     2341 ctcgccggtc agcagcagcg cgccgagctg gaacagcgcg agcagcgccg cgaggctgcc
     2401 ggcgaagccg aggatcaccg cgaacaccag catgtaggcg gcgcgctgcc agaagctctg
     2461 gcggcgcagg ttggtcttca gtcgttcgtc catcgttcgg gtacctgggc aggtggacga
     2521 ccgccggacg gcggccgggc gcgcggcacg acaggcgacg cgtcgctgca agcgtagatg
     2581 gcggcgcccg cggcggattt catcgggcgg ttgcacgccg ttacaagaag agcgcgctgc
     2641 ggcacgtggg cgtacccgtc ggcgtccgca gacgaatgtc atcggattgc cacgaatacc
     2701 gctcaaaccg atacggacgc cacgctatcc agccgcagcc ggcgcgtggc gtccgtatcg
     2761 gtgcaactcg gtacagatca gtacgatcag tacggatcgg tccggcgggg aaatccggat
     2821 cgcgatgcgg tctgattcat tcgaccttgt aggtcgcctt cgcatccttc acgcacttgt
     2881 ccttgacgtc gccgctgaac tggtcgcact tggccttggc gacttcgaaa tcggcgtcac
     2941 gcttggtttc cctggcatcc ttgcgggcat cggccgtatt gcccttttcg agcacggtga
     3001 tgtcggcctt ctgcttttcg taggtggcct tggcttcctt caggcagatg tccttcggat
     3061 cgccggagaa atcatcacag cgttccttgg caaccttgta attggcttca gcgacttctt
     3121 cggcggcttc gagacgggcc ttgctggtgc ccttcatttc cgccttggcc tgttcggtga
     3181 cgcgcgtgtg ttcggcttcg gcctgggcct tgcagacatc ctcggcattg ccggacattg
     3241 tcgaacacct ttccttgtcg gtcttgaggc gcgcatcggc ggccttgacg gcggcatcgt
     3301 actgggcctc gctcatcgcc gcctgtgcgg cggaaccgat gccgatggcc aggatgccgg
     3361 ccagaacggc gctgtacagg gcactgtgag cattacgcat gatgttccac tcccgggggg
     3421 ccgggcgcac cggtggcacg atgcgtacca ccggttgcgc atccggtttt ggtatgggca
     3481 gagaatcaag ggcccgcatc aggacgggcg ggtgccgttt ccggcaccgg gcgaatcacg
     3541 aaggaattac agcgtgcgcg tgtattcgtc gacttcgcgc ttggcttcat ccttggtgcg
     3601 gccgtaacgt tcctgaatgc ggccggcgag gtagtcgctg ttaccttcgg cgatgtcgag
     3661 atcgtcatcg gtcagatcgc cccacttggc cttgagcgaa cccttgagct gcttccactt
     3721 gcccttgatg atgtcttcat tcatggtcgt ttcctcctga gggcaaatcc gttcccgctc
     3781 ggcgaaatgc cgacggtcga acctgaattt gccggtttgg ggatccgtcg ggcgggaaag
     3841 cattcccgtc gtgtcccgac ggcggtaaag accggggcat ttcgcgggta gttccgcagg
     3901 cgtcattttt ttcgatcgaa aagcacagat tccctgatta aaaatcgttc atgtcggggg
     3961 ctgcggccgt gtggccacgg ctggctatgc tgccggaatc agggatttcg ccgccgcacg
     4021 gaggccccgc cgcatgtcgc tcgctctcgc ccactgccgc gcccagctcg gcatcgacgc
     4081 gccgccggtc gcggtcgagg tccacctgtc gaacggcctg ccggggctgg cgatcgtcgg
     4141 cctgccggag gcggccgtga aggagagcaa ggaccgcgtg cgcggcgcga tcctgaactc
     4201 gcgccacgaa tatccgcagc ggcgcatcac cgtgaatctg gccccggccg acctgccgaa
     4261 ggaaggcggg cgcttcgacc tgccgatcgc gctcggcatc ctcgccgcca gcggccagct
     4321 gccgggcgag ccgctcgccg ccctcgaatg cctcggcgaa ctcgcgctcg gcggcgcgct
     4381 gcgcggcgtg cgtggcgtgc tgccggcggc actggcggcg cgcgatgccg gccgggcgct
     4441 gctgctgccg gccgacaacg ccgacgaggc ggcgctggtc gagggcctga ccgtgctgcc
     4501 ggcccgtcat ctgctggagg tctgcgcgca tctgcgcggc gatgtgcggc tcgcaccgca
     4561 ccggctcgat ggtctgccgg cggccgatcc ggcgctgccg gatctggccg acgtgctcgg
     4621 gcaggcgcat gccagacgcg cgctggagat cgccgcggcc ggcgcgcaca acctgctgta
     4681 ttgcgggccg cccggcaccg gcaagtcgat gctggcggcg cgcctgccgg gcatcctgcc
     4741 gccgatgtcc gaggccgagg ccatcgagac cgcggccgtg gcctcggtca gcacgcaggg
     4801 cttccggctg caggactggc ggcggcggcc gttccgcgca ccgcatcaca cggcctcggg
     4861 cgtggcgctg gtcggcggcg gcggcaatcc gcgacccggc gagatctcgc tcgcgcacca
     4921 cggcgtgctg ttcctcgacg agctgccgga gttcaacccg cgcgtgctgg aggtgctgcg
     4981 cgagccgatg gaatccggcc acatcacgat cgcgcgtgcg gcgcgctcgg ccgactatcc
     5041 ggcgcgcttc cagctgatcg cggcgatgaa cccctgcccc tgcggctatc tcggtgacgc
     5101 cgagcgcagc tgcagctgct cgcccgagca ggtcaagcgc taccgggcgc gcgtgtccgg
     5161 cccgctgctc gatcgcatcg acctgcagct cgaagtgccg cgcctgccgc gcacggcgct
     5221 gcgcggcgag actcccgagg agccgagcgc cgtggtgcgc gtgcgcgtgc tggctgcgcg
     5281 cgaacgccag ctcgcgcgtg ccggcaaggc gaacgcgcgg ctcgaaccgc gggagctgac
     5341 gcgcgactgc cggctcggcg acgccgagca gcggctgctg gaggcggcga tggagcgcct
     5401 cggcctcagt ccgcgcgcgt atcaccgcat cctgaaggtc gcgcgcacga tcgccgatct
     5461 ggccgcgagc gaggggatca ccacggcgca cctgtccgag gcaatcggct accggcggct
     5521 ggatcgcggc cgctgacgcg ctgcggagcg cgcgctaggc tggccgccgg taccggtgcc
     5581 acattccatc aggccatcag gccatcaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnc catcaggcca tcaggccatc aggagacgcg cgcgatgacg cacgacccga
     5761 actgtctgtt ctgccgcatc gtccgcggcg agattcccgc cgtgcaggtg ttcgaggacg
     5821 aggacacgat cgccttcatg gacatcttcc cggccagcga gggtcacgcg ctggtgatcc
     5881 cgaagctgca cgccgtcaac ctgctggaga tgcccgacga tgcgctcacc gccgtgcacc
     5941 gcagcgcccg gcggctggcg gcggcgatga tgcgcgggct cggaccggcc ggtttccaga
     6001 tccgccagtt caacggcgcc gaagccggac agacggtatt ccactaccac gtgcacgtcg
     6061 tgccctgcta tcccggcgcc cggcccggcc tgcacggccg tgaccaggcc gatccggcgg
     6121 tgctggccgg gctcgccgga cgcatccgcg ccgccctgtg atcgtccacg cccgcagcgg
     6181 cctgcacggt atgcgtgcgg gccgccgcgg gcggacggct tcagaggtcc gaggccgccg
     6241 gcccgggcgc ggtggcggac tctgcaccgt ccgcgggcaa ccgtgcctcc agatccgcca
     6301 cgcgccgctc cagggcttcg agccgtgccc gcgtgcgcgt cagcacctgc gtctgcacgt
     6361 cgaattcctc gcgcgtgacc agatccatgc gcgcgaacgc cgcctgcatc gcggcgcgga
     6421 aattcttttc catgtcctgc tgcagcgtgc gcagacccgg cggcacgctc tcggcgatgc
     6481 gacgggcaaa ttcgtcgatg actttcgggt cgagcatggc gggagtcctc gggggtggtt
     6541 gggtccggtc agggtatccg gccggcggga acggggtgcg tgcaccaata tggtgccctg
     6601 atatggcgca ccattccctg gtggtgcgag catgcgcgcg gctttggtgc gcccacggct
     6661 tcaaacgtct gaaacatcgg cgtggccggc caatggcatg tcctgtgctt cacgccgcaa
     6721 agcagcatac cgccggccgg ggcatcgcag cagcgggccg agacgtcacc ggtcgctgcg
     6781 cacggcgaat cagatcgaac cgcgggccat caggagctcc ctacatgaaa ctggtcactg
     6841 ccatcatcaa gccgttcaag ctcgacgacg tgcgtgaagc gctgtccgag atcggcgtgc
     6901 agggcatcac cgtcaccgag gtcaagggct ttggtcgcca gaagggccac accgaactct
     6961 atcgcggcgc cgagtacgtc gtggacttcc tgccgaaggt gaagctcgag atcgcgatcg
     7021 atgatccgct ggtcgagcgc gtcgtcgacg ccgtcaccaa ggccgccaac accggcaaga
     7081 tcggcgacgg caagatcttc gtctacgacc tcgaacaggc gatccgtatc cgcaccggcg
     7141 aggccggtgc cgccgcgctc tgatcccatc acgacagcgc ggccggaccg cgcgcacccc
     7201 ggactcatcc ttgcaggagt ttcccgacaa tgacgaataa ccccctgatc aagggcctcg
     7261 gcgcccttgg cctgctgttg ctgccggcgc tggcgctggc ccaggaagcc gccgcccccg
     7321 ccgccgcgcc gacgctcgat gccggcaaca ccgcctggat gctgacctcg acggcgctcg
     7381 tgctgttcat gaccattccc ggtctggcgc tgttctacgc cggcatggtg cgttcgaaga
     7441 acgtgctgtc ggtgatgatg cagtgcttcg cgatcaccgc gctggtgacg ctgctgtggg
     7501 tcgtctacgg ctactcggtc gccttcgaca cgaccggcat ggtcaagggc gaggtcaacc
     7561 tgtactcgtt catcggcgga ctcggcaagg ccttcatggc cggcgtcggc cgcgacagcc
     7621 tgacgctgac catcccggag tcggtgttcg cgaccttcca gatgaccttc gcgatcatca
     7681 ccccggcgct gatcgtcggc gccttcgccg agcgcatgaa gttctcggcg ctgctgctgt
     7741 acatcgtgct gtggttcacg ctgtcctacc tgccgatcgc gcacatggtc tggtcgggtg
     7801 acggcgcgct gatgtgggac tggggcgtgc tcgacttcgc cggcggcacc gtcgtgcaca
     7861 tcaacgccgg tctggccggt ttcatcgcct gcctcgtgat cggccggcgc aagggcttcc
     7921 cggtcaccgc gatgcccccg cacaacctcg gctacacgct gatcggcgcc tcgatgctgt
     7981 gggtcggctg gttcggcttc aacgccggtt cggccgtggc tgccgacggt tcggccggca
     8041 tggccatgct gaccacgcag gtcgccaccg ccgccgccgc gctggcctgg atgttcgccg
     8101 agtggatcgc gcacggtaag ccgtcggtgc tcggcatcgc ctccggcgcc gtcgccggcc
     8161 tcgtcgccgt gaccccggcc tccggcaccg ccggcccgat gggtgcgctg ctgctcggcc
     8221 tgatctccgg tctggtctgc ttcctggcct cgaccaagct caagcgcgcg atgggctacg
     8281 acgactcgct cgacgtgttc ggcgtgcacg ccgtcggtgg catcatcggc gcgatcctga
     8341 ccggcctgtt cgccgatgcg gccttcggcg gcaagggtct ggccgagggc gtctcgatcg
     8401 ccgatcagct gtggatccag accaagggcg cgctggtgac ggtcgtctac agctcgctga
     8461 tcagcttcat ctcgctgaag gtcgtcgacg cgatcgtcgg catccgcgtc accgaggaag
     8521 aagagaccca gggtctcgac atctcgctgc acgaggagcg cggctacatc ctgtaagccg
     8581 ccgccggcct gctgtcagcc gctgccgggc cttgcgcccg gccggccatc tgccgccgcg
     8641 catgtccgga gcccggacat gcgcggcctt gtttttggtt tatagtccgg ccatccccat
     8701 ctggcgcacc accccgagga cagcccccca tgaagctcgt ttccgcgatc atcaagccgt
     8761 tcaagctcga cgacgtgcgt gaagcgctgg ccgagatcgg catccagggc atcaccgtca
     8821 ccgaggtcaa gggcttcggc cggcagaaag gtcataccga gctgtaccgc ggcgccgagt
     8881 acgtcgtcga
//
LOCUS       DAOQIE010000154         8818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4024_length_8818_cov_6.397467, whole genome shotgun sequence.
ACCESSION   DAOQIE010000154 DAOQIE010000000
VERSION     DAOQIE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4024_length_8818_cov_6.397467"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK144_10585"
     CDS             <1..379
                     /locus_tag="PK144_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNF66218.1"
                     /translation="ATASPALAAAAATPPLPVANVPPPAAGAAPAPAPAGPAAPAESR
                     IELQASADSWAEVRDAGGKLLFQGTLRPGSPQSITGRPPFRMKLGHAQHVTIRLDDSL
                     VDSGVYVPKRGSVARFTLGREPG"
     gene            448..1728
                     /gene="hisS"
                     /locus_tag="PK144_10590"
     CDS             448..1728
                     /gene="hisS"
                     /locus_tag="PK144_10590"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNF66219.1"
                     /translation="MASAKNLQAIRGMNDLLPADAARWAGFEALIRDVLTGYGYREIR
                     TPIVEATELFCRSIGEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRACIEHGLLHN
                     QQQRLWYAGPMFRHERPQKGRYRQFHQIGAEAFGMDGPDIDAEMICMTARFWQRLGLA
                     DVRLQLNSLGTPACRSVYRDKLVAHLRSGYELLDEDARRRLDSNPLRILDTKNPAMRE
                     LVDAAPVLADHLDDESARHFERLRALLDAAGVGYELNPRLVRGLDYYTKTVFEWVTDR
                     LGAQGTVCAGGRYDGLVEQLGGRPVPGVGFAMGIERLLGLLEGRDDIAAATQPQVYVV
                     MAGEAAEAAGLVLAERLRDALPQLRLQLNAGGGQIRNQMKRADKSGADWALVLGEQEA
                     AQACVGLKSLRTDAAQETLAQGGIAAALAARLGL"
     gene            1787..2422
                     /locus_tag="PK144_10595"
     CDS             1787..2422
                     /locus_tag="PK144_10595"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF66220.1"
                     /translation="MDDYATEEQRVEALRDWWRQNATSILAGVVLALAGILGWQWWQQ
                     RQSGTSEQASLVYDGVLAALAKKDAEQVRTRGQALISEKPDSAYAALAALAMARSALD
                     GGDVAAALASLDWAAQHAPSPELVSIANLRAARLLIAEKRFDEAAQRLDKVGGSGLAP
                     EREELRGDLAFARGDLKAARSAWQAALAAAGPENSVLIQLKLDNLPAEEPK"
     gene            2422..3606
                     /gene="bamB"
                     /locus_tag="PK144_10600"
     CDS             2422..3606
                     /gene="bamB"
                     /locus_tag="PK144_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNF66221.1"
                     /translation="MTASVPTPLRRLAAAAVLAALSSGCSWLTSWAIGTDNSAPPAEL
                     VSIANPISIQRVWDVSIGDGAGKAFVRLQPAFDGDALYAAARDGDVAAIDAATGNKRW
                     TRDTDLAISGGVGAGAGIVVVGSSKGEIVALRQADGAVAWKAQVSSEVLAPPRIGEGI
                     VLVRVADGRFFAFDAASGRQLWNYAASVPLLSLRGASAPLIGQGIAIAGLDNGRLLLL
                     DLKSGRPVFERTLAPPRGKTEMDRLVDVDAEPRIAGSVLYIAPFHGAVTAIDLSNGST
                     LWNRDISTYAGLDVSPQQVFVTAADDTVWALDRASGSALWKNADLSGRKLSAPVAAGS
                     VVAVGDFEGYIHFLDANTGRLVGRVELDGKGIMGTPLVRNGMLFAYGKGGKLAGYRPG
                     GG"
     gene            3684..5216
                     /gene="der"
                     /locus_tag="PK144_10605"
     CDS             3684..5216
                     /gene="der"
                     /locus_tag="PK144_10605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNF66222.1"
                     /translation="MLPVIALVGRPNVGKSTLFNCLTRTRDALVADFPGLTRDRQYGL
                     GKIGPSPYLVVDTGGIVGAEAEGIDALIAQQAVKAIDEADAVLLMVDGRAGLTAADEE
                     IAAQIRRTGRPLYLVVNKTEHLDLASALADFHSLGVGAPWAISATHQRGVHSLMERVL
                     EAVSAALPEGETRELTRTTLGADDGSIRLCVVGRPNVGKSTLINRIVGEERVLAFDLP
                     GTTRDAVAVPFEKDGTRYTLIDTAGVRRRSRVDEAIEKFSVVKTLQAIEEAHVVVMVL
                     DARQGISDQDATLLGYALESGRALVIAVNKWDRLDIETRDRVRSEMARRLSFIDFATT
                     HYISALHGSGVMDLFASVKQAYGAATRKLSTPELTRLVEDAVAQHAPPLVHGHSIKLR
                     YAHQGGSNPPVIVIHGNRVEHVPDAYRRYLVNLFRKVLKLHGTPVRIEFKANVNPYEP
                     EGHVQKKQYHRPRREDREKKLAARKEAREQRKSAPATPAVPGQRPAARKPVPRKPGGR
                     KS"
     gene            complement(5358..5738)
                     /locus_tag="PK144_10610"
     CDS             complement(5358..5738)
                     /locus_tag="PK144_10610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNF66223.1"
                     /translation="MSNERRLFTRIPFVANVEVVIDGVPHPAELHDISLMGVLVRVDD
                     GASAALDTPVHVLLALDTERTVVIATHGRIVHNEDGRLGCRFDQVDLDSMIHLRRLME
                     LNLGDAERIKREIYEMVRLPEGQS"
     gene            complement(5869..>8818)
                     /locus_tag="PK144_10615"
     CDS             complement(5869..>8818)
                     /locus_tag="PK144_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66224.1"
                     /translation="AATGRLESAAHAGRVTLPAVGWRIEPQSLSTALQLPPGWRLLGA
                     EGPARADGAWLARWNLLDFFLLLLTTLAIGRLAGAAYGGLALVGLALVWHEPQAPRAL
                     WLNLAAALALLHVLPPGRLAKLAGLWRALGLAALGLACLVYAVPQVRGALYPQLAVPD
                     GMRATSVADEGFAAGGLAGNALTLNAPAAAPPPAPAAPVVQSELADEAGAGSDERTAR
                     ERAMREYEKRKSERAMKSQDSSSAPRRQQQVERYTADVQVQTGPGQPDWRWSSARLDW
                     SGPVAPEATLTLWLLSPPLAAAWRLTGVLLLFVLLWRLARCRPRPPAAAAPVEPAPIP
                     SAAAPALLLLAALGLAGLPRPAAADFPPDHLLEELRTRLSEPPACAPACATLEHLRLQ
                     LDPARLVITADYAAAADTLAALPLAGARVEPVAVTLDGQPAWLARAEDGAWAVRLPAG
                     RHRVVLDVPLPPELVTLDLPLVLAPAEVEVEAEGWQVSGVRNGRAGDGNLRLLRAQDD
                     SAGETRLEAGTLPPWLRVTRELVLGPADWSVETTVERLSQPGAAIGVEIPLLPGERPT
                     GAGLRIEHGRLALTLGPEDTESRWSSTLARSDTLTLSAGDGPWSEVWRLSAAPLWHVE
                     SDGTPLVHRYTAGNGDWQPEWRPRPGEALKLAISRPEGVPGAVVTLDAARLTLTPGPD
                     GALAILDLRVRASRGLDHLLGLPEGALVQQVLADGRPLALSTVEGRLLLPVRPGAQEL
                     RIVWRQPGALGAHYVTPLPDPGLPGANLRVDVQVPADRWVLLTGGPRLGPAVLFWGVL
                     AVLAAGAVALARLRFAPLGLIGWLLLAIGLAPLDPLLLLVPVGWFALCTWRERHGARL
                     GPLAFNALQLGLALATFALLAVLVLAVQQGLLGAPDLQIAGNGSSGHLLRWYQDRSPP
                     DWQPGTFWSAPLWVYRLLMLGWALWLAWAVLGWLRWGWQCWGRDGHWRRRTSGRTAAA
                     TVWTDGNASE"
BASE COUNT         1281 a         3244 c         3191 g         1102 t
ORIGIN      
        1 ggccacggcc tcgccggcgc tggcggcggc ggcggcgacg ccgcccctgc cggtcgcgaa
       61 cgtaccgcca ccggctgcgg gtgctgcgcc ggcgccagca ccggctggcc cggcggctcc
      121 ggccgagtcg cggatcgagc tgcaggccag tgccgacagc tgggccgagg tgcgcgatgc
      181 cggcggcaag ctgctgttcc agggcacgct gcgtccgggc agcccgcagt cgatcaccgg
      241 ccggccgccg tttcgcatga agctcggtca cgcgcagcac gtcacgatcc ggctcgacga
      301 cagcctcgtc gactccgggg tctacgtgcc gaagcgcggt tccgtcgcgc gcttcacgct
      361 cggccgcgaa ccgggctgaa gcggctgccg cccggcattc ctcccgacac cttcccttcc
      421 cgaccttccc cacatacagc gaacacgatg gcgagcgcga agaacctgca agcgatccgc
      481 ggcatgaacg acctgctgcc ggccgatgcc gcccgctggg ccggcttcga ggcgctgatc
      541 cgcgacgtgc tcaccggcta cggctatcgc gagatccgca cgccgatcgt cgaggccacg
      601 gaactcttct gccgctcgat cggcgaggtc accgacatcg tcgagaagga gatgtacacc
      661 ttcgccgacc gcaacggtga cagcctgacg ctgcgccccg agggcacggc gagctgcgtg
      721 cgcgcctgca tcgagcacgg cctgctgcac aaccagcagc agcggctctg gtacgccggg
      781 ccgatgttcc gccacgagcg cccgcagaag ggccgctacc ggcagttcca ccagatcggc
      841 gccgaagcct tcggcatgga tggcccggac atcgatgccg agatgatctg catgactgcg
      901 cgcttctggc agcggctcgg actcgccgac gtgcgcctgc agctgaactc gctcggcacg
      961 ccggcctgtc gcagcgtcta tcgcgacaag ctggtcgcgc atctgcggag cggttacgag
     1021 ctgctcgacg aggacgcgcg ccggcggctc gacagcaatc cgctgcgcat cctcgatacc
     1081 aagaacccgg cgatgcgtga actcgtcgac gctgcgccgg tgctcgccga tcacctcgac
     1141 gacgaatcgg cgcggcactt cgagcgcctg cgcgcgctgc tcgatgccgc cggcgtcggc
     1201 tacgagctca atccgcggct ggtgcgcggg ctcgactact acaccaagac cgtgttcgag
     1261 tgggtcaccg accgcctcgg tgcccagggc acggtctgcg ccggcggccg ctacgacggc
     1321 ctcgtcgagc agctcggcgg caggccggtg ccgggcgtcg gcttcgcgat gggcatcgag
     1381 cgcctgctcg gcctgctcga aggccgcgac gacatcgccg ccgccacgca gccgcaggtg
     1441 tatgtggtca tggccggcga ggcggccgaa gcggcgggtc tcgtgctcgc cgagcgcctg
     1501 cgcgacgcgc tgccgcagct tcgcctgcag ctcaacgccg gcggcggcca gatcaggaac
     1561 cagatgaagc gcgccgacaa gtccggtgcc gactgggcgc tggtgctcgg cgagcaggag
     1621 gccgcgcaag cctgcgtcgg cctgaagtcg ctgcgcaccg acgccgccca ggaaaccctg
     1681 gcgcagggcg gcatcgccgc ggcgctggcc gcgcggctcg gcctctgagc cttccccgaa
     1741 cccgttaccc ggccgcacgg cggcggagct gaatcccgac atggcgatgg acgactacgc
     1801 aaccgaggaa caacgcgtcg aggcgctgcg cgactggtgg cgccagaacg ccacctcgat
     1861 cctggccggc gtcgtgctgg cgctggccgg catcctcggc tggcagtggt ggcagcagcg
     1921 ccagtccggc acctccgaac aggcttcgct ggtctacgac ggcgtgctgg cggcgctcgc
     1981 gaagaaggac gccgagcagg tgcgcacgcg cggccaggcg ctgatctcgg agaaacccga
     2041 ctcggcctat gccgcgctgg cggcactggc gatggcgcgc tcggcgctcg atggcgggga
     2101 tgtggccgcc gcgctcgcga gcctcgactg ggccgcgcag cacgcgccgt cgccggaact
     2161 cgtcagcatc gccaacctgc gcgcggcgcg gctgctgatc gccgagaagc gcttcgacga
     2221 ggccgcgcag cgcctcgaca aggtcggcgg cagcggactt gcgcccgagc gcgaggaact
     2281 gcgcggcgac ctggccttcg cgcgcggcga cctgaaggcc gcccgcagtg cctggcaggc
     2341 cgcgctcgcc gccgccggcc ccgagaacag cgtgctgatc cagttgaagc tcgacaacct
     2401 gccggccgag gagccgaagt gatgaccgcc tccgttccga cgccgctgcg ccgcctcgcc
     2461 gccgctgccg tgcttgccgc actgtccagc ggctgcagct ggctgacctc ctgggccatc
     2521 ggcaccgaca actcggcgcc gccggccgag ctcgtgagca tcgccaaccc gatctcgatc
     2581 cagcgcgtct gggacgtctc gatcggcgac ggcgccggca aggccttcgt gcgcctgcag
     2641 ccggccttcg acggcgatgc gctgtacgcg gccgcgcgcg acggtgatgt tgccgccatc
     2701 gatgccgcga ccggcaacaa gcgctggacc agggacaccg atctcgccat cagcggcggt
     2761 gtcggggccg gggccggcat cgtcgtggtc ggcagcagca agggcgagat cgtcgcgctg
     2821 cgccaggccg acggcgccgt cgcctggaag gcgcaggtat cgagcgaggt gctcgcgccg
     2881 ccgcgcatcg gcgagggcat cgtgctcgtg cgcgtcgccg acgggcgctt cttcgcgttc
     2941 gatgccgcga gcggccgcca gctgtggaac tacgccgcca gcgtgccgct gctgtcgctg
     3001 cgcggggcct cggcgccgct gatcgggcag ggcatcgcga tcgccggcct cgacaacggc
     3061 cggctgctgc tgctcgatct gaagtccggc cggccggtct tcgagcgcac gctggcaccg
     3121 ccgcgcggca agacggaaat ggaccggctg gtcgatgtcg atgccgagcc gcgcatcgcc
     3181 ggttcggtgc tgtacatcgc gcccttccac ggcgccgtga ccgcgatcga cctcagcaac
     3241 ggctcgacgc tgtggaaccg cgacatctcg acctacgccg gtctcgatgt ctcgccgcag
     3301 caggtgttcg tgaccgctgc cgatgacacg gtctgggcgc tggaccgcgc cagcggctcg
     3361 gcgctctgga agaacgccga tctctccggg cgcaagctca gcgccccggt cgccgccggc
     3421 agcgtggtcg cggtcggtga cttcgagggc tacatccact tcctcgacgc gaacaccggc
     3481 cggctggtcg ggcgcgtcga actcgacggc aagggcatca tgggcacgcc gctggtgcgc
     3541 aacggcatgc tgttcgcgta cggcaagggc ggcaagctcg ccggctaccg ccccggcggc
     3601 ggctgagccc agcccttccg atcctcgtca ttccccgccg ggccccgagt ggcccggctt
     3661 cgttttttac ggaggaccgc agcatgctcc cggtcatcgc cctcgtcggc cgccccaacg
     3721 tcggcaagtc gacgctgttc aactgcctca cgcgcacgcg cgatgcgctg gtcgcggact
     3781 tccccggcct gacccgcgac cgccagtacg ggctcggcaa gatcggcccg agcccgtatc
     3841 tggtcgtcga caccggcggc atcgtcggtg ccgaggccga aggcatcgat gcgctgatcg
     3901 cgcagcaggc ggtcaaggcc atcgacgagg ccgatgccgt gctgctgatg gtcgacggcc
     3961 gcgccggcct gaccgcggcc gatgaggaga tcgccgcgca gatccgccgc accggccggc
     4021 cgctgtacct cgtcgtcaac aagaccgagc acctcgacct cgccagcgcg ctcgccgact
     4081 tccactcgct cggcgtcggt gcaccgtggg cgatctcggc cacgcaccag cgcggcgtgc
     4141 attcactgat ggagcgcgtg ctcgaagccg tcagcgccgc gctgcccgag ggcgagacgc
     4201 gcgagctcac ccgtaccacg ctcggcgccg acgacggctc gatccggctg tgcgtggtcg
     4261 ggcggcccaa cgtcggcaag tcgacgctga tcaaccgcat cgtcggcgag gagcgcgtgc
     4321 tcgccttcga cctgccgggc acgacccggg acgcggtcgc cgtgcccttc gagaaggacg
     4381 gcacgcgcta cacgctgatc gacaccgccg gcgtgcgccg gcgctcacgc gtcgacgagg
     4441 cgatcgagaa gttcagcgtc gtcaagacgc tgcaggcgat cgaggaggcg cacgtcgtgg
     4501 tcatggtgct cgacgcgcgc cagggcatct cggatcagga tgcgacgctg ctcggctacg
     4561 cgctcgaaag cggccgggcg ctggtcatcg ccgtcaacaa gtgggaccgg ctcgacatcg
     4621 agacgcgcga ccgcgtgcgc tcggaaatgg cgcgccgcct gtcattcatc gacttcgcga
     4681 ccacgcacta catctcggcg ctgcacggct cgggcgtcat ggacctgttc gcgtcggtga
     4741 agcaggccta cggcgcggcg acccgcaagc tgtcgacgcc ggagctgacg cgcctcgtcg
     4801 aggacgccgt cgcccagcac gcgccgccgc tcgtgcacgg tcacagcatc aagctgcgct
     4861 acgcccacca gggcggcagc aatccgccgg tcatcgtcat ccacggcaac cgcgtcgagc
     4921 acgtgccgga cgcctaccgc cgctatctcg tcaacctgtt ccgcaaggtg ctgaagctgc
     4981 acggcacgcc ggtgcgcatc gagttcaagg ccaacgtcaa cccctacgaa cccgaaggcc
     5041 acgtgcagaa gaagcagtac caccgcccgc gccgcgagga ccgcgagaag aagctcgccg
     5101 cgcgcaagga agcccgcgaa cagcgcaagt ccgcccccgc caccccggcc gtccccggcc
     5161 agcgcccggc ggcccgcaag ccggtgccgc gcaagccggg cggacgcaaa agctgagcat
     5221 cctcgccccg gcgcatcccc cgcgaaccgg gtgccggggg tgcgcagact ccgcagttgc
     5281 agccgggggc gcaagaaaat gccctgccgg agcagctccg gcagggcggg ggaaaggggc
     5341 gcggcagcgg ggccgggtca ggactggcct tccggcaggc gcaccatctc gtagatctcg
     5401 cgcttgatgc gctcggcgtc gccgaggttc agctccatca gccggcgcag gtggatcatc
     5461 gagtcgaggt cgacctgatc gaagcggcag ccgaggcggc cgtcctcgtt gtgcacgatc
     5521 ctgccgtgcg tggcgatgac gacggtgcgc tcggtgtcga gtgcgagcag cacgtgcacc
     5581 ggcgtgtcga gcgcggcgct ggcgccgtcg tcgacacgca ccagcacgcc catcagcgag
     5641 atgtcgtgca gctcggcggg atgcggcacc ccgtcgatca cgacttcgac attggcgacg
     5701 aaagggatgc gcgtgaacag gcggcgttcg ttggacatgc ggagtacctg gctgttgggg
     5761 ccgcgtgtgg cagcgggatg gccggcaagc tatgcccggc aggcatgcga gtacaagcgc
     5821 tgcaatacat ggaaacaagt gcgatatcgg cggcttgcgt cagaagtttc attcgcttgc
     5881 attcccgtcg gtccagaccg tcgctgcggc cgtcctgccg gacgtgcgcc ggcgccagtg
     5941 accgtcgcgg ccccagcact gccagcccca gcgcagccag ccgagcacgg cccacgccag
     6001 ccacagcgcc cagccgagca tcagcagccg gtagacccag agcggtgccg accagaacgt
     6061 cccgggctgc cagtccggcg ggctgcggtc ctgataccag cgcagcagat ggccgcttga
     6121 accgttgccg gcgatctgca ggtccggcgc accgagcagg ccctgctgca cggcgagcac
     6181 cagcaccgcg agcagcgcga acgtcgccag cgccagcccg agctgcagcg cgttgaaggc
     6241 cagcggcccg agccgtgccc cgtggcgctc gcgccacgtg cagagggcga accagccgac
     6301 cggcaccagc agcagcagcg gatcgagcgg cgcgaggccg atcgcgagca gcagccagcc
     6361 gatcaggccg agcggtgcga agcgcaggcg cgcgagcgcc acggcgccgg ccgcgagcac
     6421 cgccagcacg ccccagaaca gcacggccgg gccgagccgc ggcccgccgg tgagcagcac
     6481 ccagcgatcg gccggcacct gcacatcgac gcgcaggttg gcaccgggca ggccgggatc
     6541 gggcagcggc gtcacgtagt gcgcaccgag ggcaccgggc tggcgccaga cgatgcgcag
     6601 ctcctgcgcg ccggggcgca ccggcagcag cagccggccc tcgaccgtgc tcagcgccag
     6661 cggccggccg tcggcgagca cctgctgcac cagcgccccc tccggcaggc cgagcaggtg
     6721 atcgaggccg cggctggcgc gcacgcgcag atcgaggatc gccagcgccc cgtccggccc
     6781 cggcgtcagc gtcagccgcg ccgcatcgag cgtgaccacg gccccgggca cgccctcggg
     6841 acggctgatc gcgagcttca gcgcctcgcc ggggcgcggg cgccattcgg gctgccagtc
     6901 gccgttgccg gcggtgtagc ggtgcaccag cggcgtgccg tcggattcga cgtgccagag
     6961 cggcgcggcc gacagccgcc agacctcgga ccacggcccg tcgccggcgc tgagcgtgag
     7021 cgtgtcgctg cgtgcgagcg tgctgctcca gcggctttcg gtgtcctcgg ggccgagggt
     7081 cagcgccagg cggccgtgct cgatgcgcag gccggcgccg gtcggacgct cgccgggcag
     7141 cagcggaatc tcgacgccga tcgccgcccc gggctgtgac aggcgctcga ccgtggtctc
     7201 gacgctccag tcggccgggc cgagcacgag ttcgcgcgtc acgcgcagcc acggcggcag
     7261 cgtgccggct tcgagccggg tttcgccggc gctgtcgtcc tgcgcgcgca gcaggcgcag
     7321 gttgccgtcc ccggcgcggc cgttgcgcac gccgctgacc tgccagccct cggcctcgac
     7381 ctcgacttcg gccggggcga gcacgagcgg caggtcgagc gtgaccagct cgggcggcag
     7441 cggcacgtcg agcacgacgc gatgccggcc ggccggcaga cgcacggccc aggcaccgtc
     7501 ctcagcgcgt gcgagccagg ccggctggcc gtcgagcgtc accgcgaccg gttcgacgcg
     7561 ggcgccggcg agcggcagcg cggcgagcgt gtcggcggcg gccgcgtagt cggcggtgat
     7621 gaccagacgg gccggatcga gctgcagccg caggtgttcg agcgtcgcgc aggcgggcgc
     7681 acaggccggg ggttccgaca ggcgcgtgcg cagttcttcg agcagatgat cgggcgggaa
     7741 gtcggccgcg gccgggcgcg gcaggccggc gaggccgagt gcggcgagca gcagcagcgc
     7801 cggggcggcg gcagacggga ttggtgccgg ttcgaccggt gccgccgcag ccggcgggcg
     7861 cggccggcag cgcgcgagcc gccacagcag cacgaacagc agcagcacgc cggtgagccg
     7921 ccaggcggcg gcgagcggcg gcgacagcag ccacagcgtc agcgtcgcct ccggtgcgac
     7981 cggaccgctc cagtcgagcc gcgcgctgct ccagcgccag tccggctggc cggggccggt
     8041 ctgcacctgc acatcggcgg tgtagcgctc gacctgctgc tgccggcgcg gagccgacga
     8101 ggagtcctgc gatttcatcg cgcgctcgct cttgcgcttc tcgtattcgc gcatcgcccg
     8161 ttcgcgggcc gtgcgctcgt cgctgccggc gccggcttcg tcggccagct cggactggac
     8221 caccggcgcg gccggtgccg gcggcggggc ggcagccggt gcgttcagcg tcagcgcgtt
     8281 gccggcgagg ccgccggcgg cgaaaccctc gtcggcgacg gacgtggcgc gcatgccgtc
     8341 gggtacggcg agctgcggat acagcgcgcc gcgcacctgc ggcacggcat agacgaggca
     8401 ggcgagcccg agcgccgcca gcccgagcgc gcgccagagg ccggcgagct tcgccagccg
     8461 cccgggcggc agcacgtgca gcagcgcgag cgcggcggcg aggttcagcc acagcgcgcg
     8521 cggagcctgg ggttcgtgcc agaccagcgc cagtccgacg agcgccagcc cgccgtaagc
     8581 cgccccggcg agccggccga tcgcgagcgt cgtcagcagc agcaggaaga aatcgagcag
     8641 gttccagcgc gcgagccagg cgccgtcggc gcgcgccggc ccttcggccc cgagcaggcg
     8701 ccagcccggc ggcagctgca gcgcggtgct gagcgattgc ggctcgatgc gccagccgac
     8761 cgccggcagc gtcacgcgcc cggcgtgcgc ggcggattcg agccgcccgg tcgcggcc
//
LOCUS       DAOQIE010000155         8812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4027_length_8812_cov_6.882037, whole genome shotgun sequence.
ACCESSION   DAOQIE010000155 DAOQIE010000000
VERSION     DAOQIE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_8812_cov_6.882037"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK144_10620"
     CDS             <1..407
                     /locus_tag="PK144_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNF66225.1"
                     /translation="MAWGLEARVPFLDHELVELAMQVPPALKLAEGGKGILKRLARGR
                     IPDGVIDRPKGYFPVPALKYVRGEFLDFMHGVLDTPAARARGVFRREYLDTLFAAPEA
                     WMTRLQGSKLWHAALFELWFQLNVDPVRGSRS"
     gene            513..836
                     /gene="grxD"
                     /locus_tag="PK144_10625"
     CDS             513..836
                     /gene="grxD"
                     /locus_tag="PK144_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNF66226.1"
                     /translation="MDDVLKRIDEQVKNNPVLIYMKGTPQFPQCGFSMRAAQALAGTG
                     KPFAYVNVLEDPEIFQNLPRYADWPTFPQIYIAGELVGGCDITVELYQNGELQKMVDA
                     VAPAE"
     gene            complement(922..2046)
                     /gene="gcvT"
                     /locus_tag="PK144_10630"
     CDS             complement(922..2046)
                     /gene="gcvT"
                     /locus_tag="PK144_10630"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNF66227.1"
                     /translation="MSAVETGLARTPLHDLHLELGARMVPFAGYSMPVQYPAGVLKEH
                     QHTRMAAGLFDVSHMGQVRLDGAQAAAALETLVPVDVLGLPAGRQRYAVFTNDAGGIL
                     DDLMIVNAGDHLFLVVNAACKAQDLAHLRARLEPAVTVTELADRALLALQGPAAAEVF
                     LRLAPAAAGMKFMDFRTLEVAGIPCFASRSGYTGEDGFEISVPAARAVELARALLAEP
                     EVAPIGLGARDSLRLEAGLCLYGHELDPTTTPIEAAIGWAIQKVRRTGGARAGGFPGA
                     DTILAQFDAGTARLRVGLAGSTRAPVREGAPLLDAGGREVGCVTSGSFGPSAGQPVAM
                     AYVETASAAPGTELSALVRGRPVPMTVTTMPFVPQRYHRG"
     gene            complement(2077..2598)
                     /locus_tag="PK144_10635"
     CDS             complement(2077..2598)
                     /locus_tag="PK144_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNF66228.1"
                     /translation="MTTNPPAAARRPVSPRLWNMLGFLACLAGMMYALYAQYFLHLEP
                     CPLCIFQRIALIALGAVFIIAALHDPQGWGSRIYALLILLVAAAGAAIAARHAWLQSL
                     PPEQVPACGPGLDYLWQNFPIADLLNTVLRGSGECAKIDWELFHLSMPWWTLFMFVGL
                     GLAGFVRNLLGTR"
     gene            complement(2705..3046)
                     /locus_tag="PK144_10640"
     CDS             complement(2705..3046)
                     /locus_tag="PK144_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNF66229.1"
                     /translation="MTDTNTDTGTDAVKNPAPAARPRIEIVYCTQCRWLLRAAWLAQE
                     LLSTFDTDLGEVALIPGTGGVFEMRCDGVTIWSRKAEGRFPEAKEVKQRVRDCIDPGR
                     DLGHSDRPRTG"
     gene            complement(3052..3774)
                     /gene="mtgA"
                     /locus_tag="PK144_10645"
     CDS             complement(3052..3774)
                     /gene="mtgA"
                     /locus_tag="PK144_10645"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNF66230.1"
                     /translation="MSDHAPESGPRRRLRLRTLLLALPLAGLLLSLLLVLPLRWLAPV
                     TSAFMIAVRLDDEAGRWPEYRWVPYEAISAQLALAVLAGEDQRFAEHHGFDTVEIERA
                     LSAADQGGKLRGASTISQQVAKNLFLWSGRSYVRKALEAWFTLLIEACWSKRRILEMH
                     LNLAQYGPRTFGAEAAAQRFFGKPAWDLDAGEAARLAAVLPNPRLYRVEAPNATVRTR
                     ERHIRTQMRNLGGTGFLDRLSE"
     gene            3850..6255
                     /locus_tag="PK144_10650"
     CDS             3850..6255
                     /locus_tag="PK144_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66231.1"
                     /translation="MFDFKRLSIRNKLMAIMLLTTGSVLALATLVMVVNEALTLERST
                     RQQLAALADVIAASARGPMSLSDPVTAYDMLEALRSKGNIVYAQLRNPDGSVFAEFRR
                     EHDARIPDYPPPGDSETGVFQEAGQLIHYQRPVLLEWRRVGTLQLAADFGELYASLGR
                     YSLLLLMMMLTTFTFAVLIAAKLQRLISEPLLALHAATQRVREQRDYSVRVHHDADDE
                     TGALVRGFNDMLEQIRQRDLELARLAERLGAEVEHRTQELSGANRELQSMVGELRSEK
                     ERAEAASLAKTQFLASISHEIRTPMNGLLGMAELLLHTALDHRQRHLSTMIQRSGRTL
                     LGIINDILDQSKIEAGRLELDAEEFALREHLEQTIELYAEDARRHGLAFRADYAPHLP
                     RRVIGDPGRLAQVLGNLLANAIKFTPHGSITVHVDALPGAGPEVTIGCEVIDTGIGIA
                     PADQARIFEAFTQTDTSATRRYGGTGLGLTIARQIVQRMGGHIGLSSAPDAGSTFWFA
                     VQLRRVQTPAALEEHALRSARVLLVGLGAVREPIHRFFRLWGAHGTDVDSAAEALALI
                     AGAAAAGEPFQVAVVGERLPDLGSSDFAAHPGTQALGLVLLHAADATAPGCEFGRCVA
                     LALPLRARPLLRALAASIASLAPAAATDPPPPAVTLLTQPKALRARVLLAEDNPINEE
                     VACIILGELGCEVHAVTDGRAAVEAWRDGHWDMIFMDGQMPVLDGIGATAEIRRLEQE
                     LDRAHTPIIALTAHAVHGDRERYLQAGMDDYVSKPFTPEDLERALAQWLGPADAQAVL
                     RAG"
     assembly_gap    6266..6365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6416..7606)
                     /locus_tag="PK144_10655"
     CDS             complement(6416..7606)
                     /locus_tag="PK144_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNF66232.1"
                     /translation="MRPLSRLPRWRPFTVLVAVLAGTGLWLWHSGHPPPPAPPPATAV
                     SAALGGGSDGAWARAYAPRPFTFPADHGAHADFRNEWWYVTGHLRAADGRRFGYQFTL
                     FRIGLHPGPLPADSAWRAQQWYLGHFAVSDLDDGRHQARERYSRAALGLAGATREPLA
                     IWLEDWRLDGGPDAGFPMRLRARDGGLSLDLQLTPLAPVVLQGEDGLSRKSSEPGNAS
                     YYYSFPRLASRGTLSLDGRNPALAVEGTSWLDREWSTSALGPGQSGWDWFALQLADGR
                     NLMLYRLRRSDGTTDPHSAGTLSDATGRTTRLSASGFTLTPEGRWTSPDGAAHYPAGW
                     RLDIPSLGLKLRVRPQLADQEMRLSVRYWEGAVAVDGHDAQGLVHGEGYLEMTRYEDG
                     PAVP"
     gene            complement(7634..>8812)
                     /locus_tag="PK144_10660"
     CDS             complement(7634..>8812)
                     /locus_tag="PK144_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF66233.1"
                     /translation="SSGIIRCRRLMPLARMAVRNLGRHLARTSAAVAALSVAFAAAFG
                     MAMMIASFRDGVNDWLLRLLNADFYAAPLAPEGGEVPPFAPGVLEALQHDPAVAALAS
                     YRRLEIPVDGRPVQLIITGLPPVARAGYKLLQGDPARAWAAFDAGSVLIGEPLATRLR
                     LGPGDELALATDHGPRRYPIAGVYTDYGSEHGRALIHPATAARDFAPGAPASAGVFLK
                     PGVDPQAARAGLEARLAPLQAVELRPNHTIVERSLAIFDRTFTITAVLRVLALVVAAA
                     GVLGTLLALALERGREFAVLRALGLLPGELLRLLAIECGLLGLAAGLLALPLGGLTGL
                     ILTDIVNRRAFGWSLPLTLPLDALVQTVLLAVIAALAAGLYPAWRLARTPPAAALREE
                     "
BASE COUNT         1281 a         3080 c         3189 g         1162 t
ORIGIN      
        1 ccatggcctg ggggctggag gcgcgcgtgc ccttcctcga tcacgagctg gtcgagctgg
       61 cgatgcaggt gccgccggcg ctcaagctcg ccgagggcgg caagggcatc ctcaagcgcc
      121 tcgcgcgcgg gcgcatcccg gacggcgtca tcgaccggcc gaagggttac ttcccggtgc
      181 cggcgctcaa atacgtgcgc ggcgagttcc tcgacttcat gcacggcgtg ctcgataccc
      241 cggcggcgcg cgcgcgcggc gtgttccgtc gggagtacct cgacacgctg ttcgcagcgc
      301 ccgaagcctg gatgacgcgg ctgcagggca gcaagctctg gcacgccgcg ctgttcgaac
      361 tgtggttcca gctcaacgtc gacccggttc gcggctcgcg aagctgattc ccctgcgggg
      421 cgtgtgcttg tccgcgtccg gcgtgcgcgt acactgccgc cccgttcccc agagcgccgg
      481 gaccgcccgg cgcgccgcag aggtaaagaa cgatggacga cgtcctcaag cgcatcgatg
      541 aacaggtcaa gaacaacccc gtgctcattt atatgaaggg cacgccgcag ttcccgcagt
      601 gcggcttctc gatgcgcgcg gcccaggcgc tcgccggcac cggcaagccg ttcgcgtacg
      661 tgaacgtgct cgaagacccg gaaatcttcc agaacctgcc gcgctacgcc gactggccga
      721 ccttcccgca gatctacatc gccggcgaac tcgtcggcgg ctgcgacatc accgtcgagc
      781 tgtaccagaa cggcgagctg cagaagatgg tcgacgcggt cgcaccggcc gaataagccg
      841 cggcgacgcg gtcttcccgc ctgcaacagc ccggctgcgg ccgggctgtg tcgtttccgg
      901 cgggcaggtt ctgaaggcgc gtcagccgcg gtgatagcgc tgcggcacga acggcatcgt
      961 cgtgaccgtc atcggcaccg gccggccgcg cacgagggcg gaaagctccg tgcccggggc
     1021 tgcgctcgcg gtctcgacgt aggccatcgc caccggctgg ccggcgctcg ggccgaagct
     1081 gccgctggtg acgcagccga cctcgcggcc gccggcatcg agtagcgggg ctccttcgcg
     1141 caccggcgcg cgcgtgctgc cggcaaggcc gacgcgcagc cgcgccgtgc cggcgtcgaa
     1201 ctgcgcgagg atggtgtccg cgcccggaaa cccgccggcg cgcgcaccgc ccgtgcgccg
     1261 caccttctgg atcgcccagc cgatcgccgc ctcgatgggg gtcgtcgtcg ggtcgagttc
     1321 gtggccgtac aggcagagcc cggcttcgag gcgcagcgaa tcgcgcgcgc cgagaccgat
     1381 cggcgcgacc tcgggctcgg cgagcagcgc acgcgccagt tcgacggcgc gggcagccgg
     1441 caccgagatc tcgaagccgt cctcgccggt gtagccggag cggctcgcga aacacgggat
     1501 gccggcgacc tccagcgtgc ggaagtccat gaacttcatg ccggcggcgg ccggggccag
     1561 acgcaggaac acctcggcgg cggccggccc ctgcagcgcg agcagtgcgc ggtcggcgag
     1621 ttccgtgacc gtgaccgccg gttcgagccg cgcgcgcagg tgcgcgaggt cctgcgcctt
     1681 gcacgcggcg ttgacgacga ggaacaggtg atcgccggcg ttgacgatca tcaggtcatc
     1741 gaggatgccg ccggcgtcgt tcgtgaatac ggcgtagcgc tggcgcccgg ccggcaggcc
     1801 gagcacgtcg accggcacca gggtttcgag cgcggcggcg gcctgtgcac cgtcgaggcg
     1861 cacctggccc atgtgcgaga catcgaacag gccggcggcc atgcgcgtgt gctggtgctc
     1921 cttcagcacg ccggcggggt attgcaccgg catcgagtag ccggcgaacg gcaccattct
     1981 cgcgccgagt tcgaggtgca ggtcgtggag cggggtgcgg gcgagaccgg tttcgacagc
     2041 gctcatgcag gcatctccgg atcaggggcg gggcgctcag cgcgtgccga gcaggttgcg
     2101 cacgaaaccg gccagaccga ggccgacgaa catgaacagc gtccaccacg gcatcgacag
     2161 gtggaacagc tcccagtcga tcttcgcgca ctcgccgctg ccccggagca cggtgttcag
     2221 caggtcggcg atcgggaagt tctgccacag gtaatcgagt cccggcccgc aggccggcac
     2281 ctgctcgggc ggcaggctct gcagccaggc gtggcgcgcg gcgatcgccg cgccggcagc
     2341 ggcgacgagc aggatcagca gcgcatagat gcgcgagccc cagccctgcg gatcgtgcag
     2401 cgcggcgatg atgaacaccg cgccgagcgc gatcagcgcg atgcgctgga agatgcagag
     2461 cggacagggt tcgaggtgca ggaagtattg cgcgtacagc gcgtacatca tgccggccag
     2521 gcaggcgagg aaacccagca tgttccagag ccggggcgac accgggcgcc gggcagcggc
     2581 gggcgggttg gtcgtcatcg gaggcagacc tcggacagga gcaggaagcg gaaaacgggc
     2641 atgcacgccg gtgcagatga cggcgattat cgcaccggtg caggaatcac gggcgcatgg
     2701 ctgctcagcc ggtgcgcggc cggtcgctgt ggccgagatc gcgcccgggg tcgatgcaat
     2761 cgcgcacgcg ctgcttgacc tccttggcct ccgggaagcg tccctcggcc ttgcgcgacc
     2821 agatcgtcac gccgtcgcag cgcatctcga acacgccgcc ggtgccggga atcagcgcca
     2881 cctcgccgag atcggtatcg aaggtgctga gcagctcctg cgcgagccac gcggcgcgca
     2941 gcagccagcg gcactgcgtg cagtagacga tctcgatgcg cgggcgggcg gcgggggcgg
     3001 ggttctttac ggcgtcggtg ccggtgtcgg tgttggtgtc ggtcatgcgg ttcactcgct
     3061 caggcgatcc agaaagcccg tgccacccag attgcgcatc tgcgtgcgga tgtggcgttc
     3121 gcgggtgcgc accgtggcgt tcggtgcctc gacccggtac agccgcggat tcggcagcac
     3181 cgccgccagc cgtgcggcct cgccggcgtc gaggtcccag gccggcttgc cgaagaagcg
     3241 ctgcgcggcg gcctcggcgc cgaaggtgcg cggaccgtac tgcgcgaggt tcaggtgcat
     3301 ctccaggatg cgccgcttgc tccagcaggc ctcgatcagc agcgtgaacc aggcctccag
     3361 ggccttgcgc acgtagctgc gcccggacca caggaacagg ttcttcgcca cctgctggct
     3421 gatcgtgctc gccccgcgca gcttgccgcc ctgatcggcc gcgctcagtg cgcgctcgat
     3481 ctcgacggta tcgaagccgt gatgttcggc gaagcgctgg tcctcgccgg cgagcacggc
     3541 cagcgcgagc tgcgccgaaa tcgcctcgta cggcacccag cggtattccg gccagcgtcc
     3601 ggcctcgtcg tcgaggcgta cggcgatcat gaacgcgctg gtcacgggcg cgagccagcg
     3661 cagcggcagc accagcagca gcgacagcag caggccggcg agcggcaggg ccagcagcag
     3721 cgtgcgcagg cgcaggcggc ggcgcggacc ggattcgggg gcgtggtcgc tcatgcgccg
     3781 agtatgcccg gcgcgcgcgg cgggcgggca tgcgtagaat cggcgcacgc ccgtcgtccg
     3841 caggcagcca tgttcgattt caagcgtctg tccatccgca acaagctcat ggcgatcatg
     3901 ctgctgacca ccggcagcgt gctggcgctg gcgacgctgg tgatggtggt caacgaggcg
     3961 ctgacgctgg agcgctcgac ccgccagcag ctcgccgcgc tcgccgacgt gatcgctgcc
     4021 agcgcccgcg ggccgatgtc gctcagcgat cccgtcaccg cctacgacat gctcgaagcc
     4081 ctgcgcagca agggcaacat cgtctacgcg cagctgcgca accccgacgg cagcgtgttc
     4141 gccgagttcc gccgcgagca cgacgcgcgc atcccggact acccgccgcc cggcgacagc
     4201 gagaccggcg tgttccagga ggccggccag ctcatccact accagcgccc ggtgctgctc
     4261 gaatggcggc gcgtcggcac gctgcagctc gccgccgact tcggcgagct gtacgcgagc
     4321 ctcgggcgct actcgctgct gctgctgatg atgatgctga ccaccttcac gttcgcggtg
     4381 ctgatcgccg ccaagctgca gcgcctgatc tccgaaccgc tgctcgcgct gcacgccgcg
     4441 acccagcgcg tgcgcgagca gcgcgactac agcgtgcgcg tgcatcacga tgccgatgac
     4501 gagaccgggg cactggtgcg cggcttcaac gacatgctcg aacagatccg ccagcgcgac
     4561 ctcgaactgg cgcggctggc cgagcgtctc ggcgccgagg tcgagcaccg cacgcaggag
     4621 ctctccgggg ccaaccgcga gctgcagtcg atggtcgggg agctgcgcag cgagaaggag
     4681 cgggccgagg cggcgagcct cgccaagacg cagttcctcg ccagcatcag ccacgagatc
     4741 cgcacgccga tgaacggcct gctcggcatg gccgagctgc tgctgcacac cgcgctcgat
     4801 catcgccagc gccacctctc gaccatgatc cagcgctccg ggcgcacgct gctcggcatc
     4861 atcaacgaca tcctcgacca gtcgaagatc gaggccgggc gcctcgaact cgacgccgag
     4921 gaattcgcgc tgcgcgagca tctggagcag accatcgagc tctacgccga ggatgcccgc
     4981 cgccacggcc tggcgtttcg cgccgactat gccccgcacc tgccgcggcg cgtgatcggc
     5041 gatcccgggc ggctcgcgca ggtgctcggc aacctgctcg ccaacgccat caagttcacg
     5101 ccgcacggct cgatcaccgt gcacgtcgat gcgctgcccg gtgccggtcc cgaggtcacg
     5161 atcggctgcg aggtcatcga caccggcatc ggcatcgccc cggccgacca ggcgcgcatc
     5221 ttcgaggcct tcacgcagac cgacacttcc gccacccgcc gctacggcgg caccggcctc
     5281 ggcctgacca tcgcccgcca gatcgtgcag cgcatgggcg ggcacatcgg cctgagcagc
     5341 gcgcccgatg ccggctcgac cttctggttc gccgtgcagc tgcgccgcgt gcagacgccg
     5401 gcggcgctgg aggagcacgc gctgcgcagc gcccgcgtgc tgctggtcgg cctcggcgcc
     5461 gtgcgcgagc cgatccaccg cttcttccgg ctctggggcg cacacggcac cgatgtcgac
     5521 agcgccgccg aagccctggc gctgatcgcc ggcgcggccg cggccggcga gccgttccag
     5581 gtggcggtgg tcggtgagcg cctgcccgat ctcggcagca gcgacttcgc cgcccacccc
     5641 ggcacgcagg cgctcgggct ggtgctgctg cacgccgccg atgccacggc gccgggctgc
     5701 gaattcgggc gctgcgtggc gctggcgctg ccgctgcgcg cccggccgct gctgcgcgcg
     5761 ctggccgcga gcattgccag cctcgcccct gcggccgcta ccgatccgcc cccgccggcg
     5821 gtcacgctgc tgacgcagcc gaaggcattg cgggcgcgcg tgctgctcgc cgaggacaac
     5881 ccgatcaacg aggaggtcgc gtgcatcatc ctcggcgaac tcggctgcga ggtgcacgcc
     5941 gtcaccgacg gccgcgccgc ggtcgaggcc tggcgcgacg gccactggga catgatcttc
     6001 atggatggcc agatgccggt gctcgacggc atcggcgcca ccgccgaaat ccgccggctg
     6061 gagcaggagc tcgaccgcgc gcacacgccg atcatcgcgc tgaccgcgca cgccgtgcat
     6121 ggtgatcgcg aacgctatct gcaggccggc atggacgact acgtcagcaa gccgttcacg
     6181 cccgaggacc tcgaacgcgc gctggcgcag tggctggggc cggccgatgc gcaggcggtg
     6241 ctgcgggcgg gctgagcggg ggctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnccgcg tgcggcatgc ccgatcccgt cgagcatccc gccgctgccc cggcctcagg
     6421 gcacggccgg cccgtcctcg tagcgcgtca tctccagata gccctcgccg tgcacgaggc
     6481 cttgcgcatc gtggccgtcg acggcgactg cgccttccca gtagcgcacc gacagccgca
     6541 tctcctgatc ggcaagctgc gggcgcacgc gcagcttcag cccgagcgac gggatgtcca
     6601 gccgccagcc ggccgggtag tgcgcggcac cgtcggggct ggtccagcgg ccttcggggg
     6661 tgagggtgaa gccggaggcg gagaggcgcg tcgtgcggcc ggtggcgtca ctgagcgtgc
     6721 cggccgagtg cgggtcggtc gtgccgtcgc tgcggcgcag gcggtacagc atcaggttgc
     6781 ggccgtcggc gagctgcagc gcgaaccagt cccagccgct ctggccgggg ccgagcgcgc
     6841 tggtgctcca ttcgcgatcg agccagctcg tgccctcgac cgcgagcgcg gggttccggc
     6901 catcgaggct cagcgtgccg cggctcgcga ggcgcgggaa cgagtagtag tacgaggcgt
     6961 tgccgggctc gctgctcttg cggctcaggc cgtcctcacc ctgcagcacc acgggtgcga
     7021 gcggcgtgag ctgcagatcg agcgacaggc cgccgtcacg ggcacgcagg cgcatcggga
     7081 agccggcatc gggaccgccg tcgaggcgcc agtcctcgag ccagatcgcc agcggttcgc
     7141 gggtggcacc ggcgaggccg agcgcggcgc ggctgtagcg ttcgcgggcc tgatgccggc
     7201 cgtcgtcgag atccgagacc gcgaagtggc cgaggtacca ctgctgcgcg cgccaggccg
     7261 agtcggccgg cagcgggccg gggtggaggc cgatgcggaa cagcgtgaac tggtagccga
     7321 agcggcggcc atcggcggcg cgcaggtgac cggtcacgta ccaccattcg ttgcggaagt
     7381 ccgcgtgcgc gccgtgatcg gccggaaacg tgaaggggcg cggcgcgtat gcgcgtgccc
     7441 aggcgccgtc gctgccgccg ccgagggcag ccgagacggc ggtggccggt ggcggggccg
     7501 gtggtggcgg gtggccgctg tgccagagcc agagccccgt gccggcgagc acggcgacga
     7561 ggacggtgaa cggacgccag cgcggcagcc ggctgagcgg gcgcatcgct ttcattcctc
     7621 gttcattcct ctttcattcc tcgcgcagcg ccgcggccgg cggcgtgcgt gccagccgcc
     7681 aggccgggta caggccggcg gcgagcgcgg cgatgaccgc gagcagcacg gtctgcacga
     7741 gcgcatcgag cggcagcgtc agcggcagcg accagccgaa ggcgcggcgg ttgacgatgt
     7801 cggtcaggat caggccggtc agtccgccga gcggcagcgc cagcagtccg gcggccagcc
     7861 cgagcaggcc gcattcgatg gcgagcaggc gcagcagctc gcccggcagc aggccgagcg
     7921 cgcgcagcac cgcgaattcg cgcccgcgtt cgagcgcgag cgcgagcagc gtgccgagca
     7981 cgccggcggc ggcaaccacc agcgccagca cgcgcagcac ggcggtgatc gtgaaggtgc
     8041 gatcgaagat cgccagcgag cgctcgacga tcgtgtggtt cgggcgcagc tcgaccgcct
     8101 gcagcggcgc gaggcgcgct tcgagtccgg cgcgcgccgc ctgcggatca acgcccggtt
     8161 tcaggaacac gccggccgag gccggtgcgc ccggtgcgaa gtcgcgcgcg gcggtggccg
     8221 ggtggatcag cgcgcggccg tgttcggagc cgtagtcggt gtagacgccg gcgatcgggt
     8281 agcggcgcgg gccgtggtcg gtcgcaagcg cgagttcatc ccccggaccg agccgcaggc
     8341 gcgtcgccag cggttcgccg atcagcacgc tgccggcatc gaaggccgcc caggcgcggg
     8401 ccgggtcgcc ctgcagcagc ttgtagccgg cgcgcgccac cggcggcagc ccggtgatga
     8461 tcagctgcac cggccggccg tcgaccggaa tctccagccg ccggtagctg gcgagcgcgg
     8521 cgacggccgg atcgtgctgc agcgcttcga gcacgccggg tgcaaacggc ggcacctcgc
     8581 cgccttcggg ggcgagcggc gcggcgtaga agtcggcgtt cagcagcctg agcagccagt
     8641 cgttcacgcc gtcgcggaag ctcgcgatca tcatcgccat gccgaaggcg gcggcgaagg
     8701 ccaccgacag cgcggcgacc gcggcgctgg tgcgcgcgag atgccggccg aggttgcgca
     8761 cggccatgcg cgcgagcggc atcagccggc gacacctgat tatcccgctg ct
//
LOCUS       DAOQIE010000156         8808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4031_length_8808_cov_8.052553, whole genome shotgun sequence.
ACCESSION   DAOQIE010000156 DAOQIE010000000
VERSION     DAOQIE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_8808_cov_8.052553"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1542)
                     /gene="glnG"
                     /locus_tag="PK144_10665"
     CDS             complement(133..1542)
                     /gene="glnG"
                     /locus_tag="PK144_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNF66234.1"
                     /translation="MARKEHVWVIDDDHSIRWVLEKALSRETMAVSAFDSGNAALAAL
                     ERGSAPDAIVADIRMPGINGLELLARLREQVPDLPVIIMTAHSDLDSAVAAYQGGAFE
                     YLPKPFDVDEAVALVRRACDLRRQQAASADHGGPMPEIIGEAPAMQEVFRAIGRLSRS
                     NITVLINGESGTGKELVARALHRHSPRAERPFIALNMAAIPRDLLESELFGHERGSFT
                     GAQAARQGRFEQADGGTLFLDEIGDMPSELQTRLLRVLAEGEFYRVGGHSPIKVDVRV
                     IAATHQDLENRVRQGQFREDLFHRLNVIRIHLPPLRKRREDIPLLMAHFLKLAARELD
                     VEPKTLRSDVEGFLQQLDWPGNVRQLENLCRWLTVMASGREVHMEDLPSELKLTPIGA
                     SVGGEGGGDWRQALSDWAAATLIRGESEILNTALPEFERTLINVALKHSGGHRQDAAR
                     LLGWGRNTLTRKIKELNME"
     gene            complement(1556..2617)
                     /gene="glnL"
                     /locus_tag="PK144_10670"
     CDS             complement(1556..2617)
                     /gene="glnL"
                     /locus_tag="PK144_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNF66235.1"
                     /translation="MTAVVRTDYPRRVTDNLTTGVVVLDRHLRTVYMNPAAEMLFALS
                     FRQALGAHFRDLAIGADNLEAGMRRCLDSGHPYTERELTLTLPGEHVITVDSSVTPVV
                     EHEQILELLVELRQVDRQLRISREENLIAQHTTTQVLVRNLAHEIKNPLGGLRGAAQL
                     LERELPDERLKEYTRIIIHEADRLRGLVDRMLGPMHPPARQAVNLHEVLERVRTLVVA
                     EGLGSTEIVRDYDPSIPPLQGDFDQLVQAVLNIVRNASQALAGTGTITLRTRVTRQCT
                     IGSRRYKLVARLDIVDDGPGIPPAIKERIFYPMITGRPDGTGLGLSIAQGLVSQHGGL
                     IECNSVPGDTTFTLLLPLE"
     gene            complement(2948..4357)
                     /gene="glnA"
                     /locus_tag="PK144_10675"
     CDS             complement(2948..4357)
                     /gene="glnA"
                     /locus_tag="PK144_10675"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HNF66236.1"
                     /translation="MSAEDVLSKIKDEGIKFVDFRFTDTRGKEQHVTVPAHTVDADVF
                     ESGKMFDGSSISGWKGINESDMILMPDPESAFVDPFFDEPTINITCDVIEPATMQGYE
                     RDPRSVARRAEAYLQSTGIADTAYFGPENEFFIFDDVRYSTEIGRVGYQIDSEEAAWN
                     TNKQYSDGNTGHRPGIKGGYFPVPPVDSLHDIRAAMCLALAEIGQRVEVHHHEVATAG
                     QCEIGVGFHTLVRKADEVQSLKYIIQNVAHNYGKTATFMPKPLHGDNGSGMHVHQSLA
                     KDGKNLFTGDLYGGLSEMALFYIGGIIKHAKALNAFTNASTNSYKRLVPHFEAPVMLA
                     YSARNRSASIRIPFVSNPKGRRIEVRFPDSTANPYLAFAAMMMAGLDGIQNKIHPGEA
                     MDKDLYDLPPEEEALIPQVCFSLDQALAELDKDREFLTKGGVFTDDLIDAYIGLKNQE
                     VQKVRMIPHPAELELYYSL"
     gene            4783..5967
                     /locus_tag="PK144_10680"
     CDS             4783..5967
                     /locus_tag="PK144_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF66237.1"
                     /translation="MNAPHAAAPHRPRVGLLLTGGGARAAYQVGVLQAIAEWLPPGAP
                     TPFAVISGSSAGAINAALLASHAGAFATGVDALSRLWGRLACSDVFRTDWRAALVSGL
                     LWTGSLAAGGLGRLTPRSLLDSRPLRALLGRHIDFAAVRTAIEDGTLHAFSLSAAGYN
                     SGQSITFFMGHAGLRPWQRTRRIGMPRPLTLDHLMASCAIPFVFPAVRIGYDWYGDGS
                     MRQIAPLSAPLHLGADRLLVIGVREETPDEPDRPILRLEYPSPGQIAGYILDTLFMDA
                     LSADIERARRINRTLALIPPERRAQLALRPIEIATVFPSRDVRLIAAHHEAHFPRSVR
                     YLLHSLGATRGRGRPLISYLLFEGGFCGELIALGYRDAQDARERLCTLLGLAHPAATL
                     RP"
     gene            6144..7073
                     /gene="glyQ"
                     /locus_tag="PK144_10685"
     CDS             6144..7073
                     /gene="glyQ"
                     /locus_tag="PK144_10685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNF66238.1"
                     /translation="MRDVSTFQGLILALQEYWAEQGCAVLQPYDMEVGAGTFHTATTL
                     RAVGPEPWAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDDIQDLYLGSLAR
                     LGIDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCRPVTGE
                     ITYGLERIAMYLQNVESVYDLTWTDGPFGRISYGDVFHQNEVEQSTYNFEQADTAWLF
                     RQFDQCESESARLVALGLALPAYEQMLKASHTFNLLDARKAISVTERQRFILRVRTLA
                     RACAQAYHDARAALGFPMCRTPAAITATTGTGA"
     gene            7076..>8808
                     /gene="glyS"
                     /locus_tag="PK144_10690"
     CDS             7076..>8808
                     /gene="glyS"
                     /locus_tag="PK144_10690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNF66239.1"
                     /translation="MSQVETNDLLVEIGTEELPPKALPALSEAFEAGIRDGLAKAGLN
                     FGAVHRYAAPRRLAVLVHELPMQQPERRLERRGPAVSAAFGPDGTPTKATEGFARSCG
                     VEVAALTRVATDKGEWLAHVSTEPGAPLAQLLPGIVEAALAALPIPKRMRWGARTQEF
                     VRPAHWIVLLHGTQVVPGEILGLPTGRETRGHRFHHPAPIAIPKPAAYAPLLADQGRV
                     LADFASRREAIRNQAVRAAAGLGGEAVIRPELLDEVTALVEWPVVLVGSFEPRFLAVP
                     NEALISTMQDNQKYFPVVDAQGRMLPHFITIMNIESRDPAQVRAGNERVIRPRFSDAE
                     FFWNQDRKRRLDSHVESLKTVVFQQKLGTLHDKSGRVAQLAQTLAGVLGADAALAARA
                     AQLAKCDLMTNMVGEFPELQGTMGRYYAAHDGEAAELAQALEEQYRPRFAGDAIATSA
                     TGRIVALADRLDTLVGIFAIGQPPSGAKDPFALRRAALGALRTIIEGGLEIDLHATLT
                     GAAAAFPAALAAPAAVDAVFDFMIERLRAFYTERVGEPVRPDEFDAVAACRPTRPLDF
                     DRRLRALAAFRR"
BASE COUNT         1310 a         3068 c         3020 g         1410 t
ORIGIN      
        1 cgtccgaagt cgtccgaggg aatccgaggc cagccggtgc gcaaacggta catctgacgg
       61 tacatctagc cggattcgcg ccggcaacaa agacaattgc cattgatttt caatgaaaat
      121 acccggcaat gatcattcca tgttcagttc cttgatcttg cgcgtcagcg tgttgcggcc
      181 ccagccgagc agccgggcgg cgtcctggcg gtggccgccg gagtgcttca gcgcgacgtt
      241 gatcagcgtg cgctcgaact ccggcagcgc ggtgttgaga atctcgctct cgccgcggat
      301 cagcgtcgcc gcggcccagt ccgacagcgc ctgacgccag tcgccgccgc cctcgccgcc
      361 gacgctcgcg ccgatcggcg tcagcttcag ctcgctcggc aggtcctcca tgtgcacttc
      421 gcggcccgag gccatcaccg tcagccagcg gcacaggttt tcgagctggc ggacgttgcc
      481 gggccagtcg agctgctgca ggaagccttc gacatccgag cgcagcgtct tcggctccac
      541 gtcgagttcg cgcgccgcga gcttcaggaa gtgcgccatc agcagcggga tgtcctcgcg
      601 gcgcttgcgc agcggcggca ggtggatgcg gatgacgttc agccggtgga acaggtcctc
      661 gcggaactgc ccctgacgca cccggttctc gaggtcctga tgggtcgcgg cgatcacgcg
      721 cacgtcgacc ttgatcgggc tgtgaccgcc gacgcggtag aactcgccct cggcgagcac
      781 gcgcagcagc cgcgtctgca gctccgaggg catgtcgccg atctcgtcga ggaacagcgt
      841 gccgccgtcg gcctgctcga agcggccctg acgcgcggcc tgcgcgccgg tgaaggagcc
      901 gcgctcgtgg ccgaacagct cggattcgag caggtcgcgc gggatcgccg ccatgttcag
      961 tgcgatgaac ggccgctcgg cgcgcgggct gtgccggtgc agcgcgcggg cgaccagctc
     1021 cttgccggtg ccggattcgc cgttgatcag caccgtgatg ttcgagcgcg acagccggcc
     1081 gatggcgcgg aacacctcct gcatcgccgg cgcctcgccg atgatctccg gcatcggtcc
     1141 gccgtgatcg gcgctggccg cctgctggcg gcgcaggtcg caggcgcggc gcaccagcgc
     1201 gacggcttca tcgacatcga agggcttggg caggtactcg aaggcaccgc cctgataggc
     1261 ggcgacggcg ctgtcgaggt cggagtgcgc ggtcatgatg atgaccggca ggtccggcac
     1321 ctgctcgcgc agccgcgcca gcagttcgag gccgttgatg ccgggcatgc ggatgtcggc
     1381 gacgatcgca tccggcgcgc tgccgcgttc gagcgcggcg agcgcggcgt tgccgctgtc
     1441 gaaggccgaa accgccatcg tctcccggct cagggccttt tccagcaccc agcggatcga
     1501 atggtcgtcg tcgatgaccc atacgtgttc ttttctggcc atggcgggct ctcgcttact
     1561 ccagcggcag cagcaacgtg aaggtggtgt cgccgggcac gctgttgcac tcgatcaggc
     1621 cgccgtgctg gctcaccagt ccctgggcga tggacaggcc gagcccggtg ccgtcggggc
     1681 ggccggtgat catcgggtag aagatgcgtt ccttgatcgc cggcgggatg ccgggaccgt
     1741 cgtcgacgat gtcgaggcgc gcgaccagct tgtagcggcg cgagccgatc gtgcactggc
     1801 gcgtcacgcg cgtgcgcagc gtgatcgtgc cggtgccggc gagcgcctgc gaggcgttgc
     1861 gcacgatgtt gagcaccgcc tgcaccagct ggtcgaagtc accctgcagc ggcgggatgc
     1921 tcggatcgta gtcgcgcacg atctcggtgc tgccgagccc ttcggcgacc accagcgtgc
     1981 gcacgcgctc cagcacctcg tgcaggttca cggcctggcg cgccggcggg tgcatcgggc
     2041 cgagcatgcg atcgacgagg ccgcgcaggc ggtcggcctc gtggatgatg atgcgggtgt
     2101 attccttcag gcgctcgtcc ggcagctcgc gttcgagcag ttgcgcggcc ccgcgcaggc
     2161 cgccgagcgg gttcttgatc tcgtgggcga ggttgcgcac cagcacctgc gtggtcgtgt
     2221 gctgcgcgat caggttctcc tcgcgcgaga tgcgcagctg ccggtcgacc tgacgcagct
     2281 cgaccagcag ttcgaggatc tgctcgtgct cgaccaccgg cgtgaccgag ctgtcgacgg
     2341 tgatcacgtg ctcgccgggc agtgtcagcg tcagctcgcg ctcggtgtac ggatggccgc
     2401 tgtcgaggca gcggcgcatg ccggcttcga ggttgtcggc cccgatcgcc agatcgcgga
     2461 aatgcgcgcc gagcgcctgc cggaaggaca gcgcgaacag catctcggcc gccggattca
     2521 tgtagaccgt gcgcagatga cggtcgagga ccacgacgcc ggtcgtcagg ttgtccgtca
     2581 cgcggcgcgg ataatcggtt ctcaccacgg ctgtcatctc cggatacatg ccgtcgggtt
     2641 tagcaagaag tggtccaacc gggtgcaagc gccggaatcc cgtccggttt tttgggccgg
     2701 cgcgcagttg cagcgcgcca gcggcgggca gtggcgcacg gcgatccgga cgcgatgcac
     2761 catcatcaac cacagcaccg ctttggtgcg catcctggcg gtgcaaacct ttgctgcacg
     2821 atcgtgcagc cgggcggtcc gcgccacgat cgcgcccgcc agctgcgggc aagaaaaaac
     2881 cccgtgccgc agggcacggg gttccggcgc cgggcggccg cgtgcagcgg ccgtcagcgg
     2941 agcgatctca gaggctgtag tacagctcga gctcggccgg gtgcgggatc atgcgcacct
     3001 tctggacttc ctgattcttc aggccgatgt aggcatcgat caggtcgtcg gtgaacacgc
     3061 cgcccttggt caggaactcg cggtccttgt ccagctcggc cagcgcctgg tcgaggctga
     3121 agcagacctg cgggatcagg gcttcctctt ccggcggcag gtcgtacaga tccttgtcca
     3181 tggcctcgcc cggatggatc ttgttctgga tgccgtcgag accggccatc atcatcgccg
     3241 cgaacgccag gtagggattg gcggtggaat ccgggaagcg cacctcgatg cggcggccct
     3301 tcggattcga cacgaacggg atgcggatcg acgccgagcg gttgcgggcc gagtaggcca
     3361 gcatgaccgg ggcttcgaag tgcggcacca ggcgcttgta gctgttggtc gaggcgttcg
     3421 tgaacgcgtt cagcgccttg gcgtgcttga tgatgccgcc gatgtagaac agcgccattt
     3481 ccgacaggcc gccgtacagg tcgccggtga acaggttctt gccgtccttc gccagcgact
     3541 ggtgcacgtg catgcccgag ccgttgtcgc cgtgcagcgg cttcggcatg aaggtcgccg
     3601 tcttgccgta attgtgcgcg acgttctgga tgatgtactt cagcgactgc acttcgtcag
     3661 ccttgcgcac cagcgtgtgg aagccgacgc cgatctcgca ctggccggcg gtcgcgactt
     3721 cgtggtggtg cacttcgacg cgctggccga tctcggcgag cgccaggcac atcgcggcgc
     3781 ggatgtcgtg caggctgtcg accggcggca ccgggaagta gccgcccttg atgcccggac
     3841 ggtgaccggt gttgccgtcg ctgtactgct tgttggtgtt ccaggcggct tcttcggagt
     3901 cgatctggta gccgacgcgg ccgatctcgg tcgagtagcg cacgtcgtcg aaaatgaaga
     3961 attcgttctc cgggccgaag tacgcggtat cggcgatgcc ggtgctctgc aggtaggcct
     4021 cggcgcggcg cgccaccgag cgcggatcac gctcgtagcc ctgcatcgtc gccggttcga
     4081 tgacgtcgca ggtgatgttg atcgtcggtt cgtcgaagaa cggatcgaca aaggcggatt
     4141 ccggatccgg catgaggatc atgtcggact cgttgatgcc cttccagccg gagatcgacg
     4201 agccgtcgaa catcttgccg ctctcgaaca cgtccgcatc gacggtgtgc gccgggacgg
     4261 tgacgtgctg ctccttgccg cgcgtgtccg tgaagcggaa gtccacgaac ttgatacctt
     4321 cgtctttgat cttgctcaga acgtcttcgg cagacatcta gtttcctccg cgatgagaac
     4381 gggctttgac tcagcgattc aaaaacaaca aaaagcatga aaacgaagtt ttcggcgagg
     4441 catcggttca gcacgataca tgccactcgt gccgaggcac ccgatccggt catccggcag
     4501 ggggccatgc accgccacga ccggctgatg cacgataaca gtgcaaaaag aagtgcatcc
     4561 gcaccggcag ggagcggtat tgacggcttg ttgcgcttca cccctcgcgc gcatcccgcg
     4621 gcggcgtatg ttgccactgc tgccggaaaa attgcatccc ggcaaggttc ggaccccatc
     4681 tgtggataag tttctggata aaccctccgg ttatccacat gggcatgtcg cagtgcagcg
     4741 cgaatgccgg cagcacccgg cacggcgcgg acgcggcaac ccatgaacgc accgcacgca
     4801 gccgcaccgc accggccgcg ggtcgggctg ctgctgaccg gcggcggcgc gcgcgcggcg
     4861 tatcaggtcg gcgtgctgca ggcgatcgcc gaatggctgc cgcccggcgc gccgacgccg
     4921 ttcgcggtga tctccggcag ctcggccggg gccatcaacg ccgcgctgct cgcaagccac
     4981 gccggcgcct tcgcgaccgg tgtcgacgca ctctcccggc tgtggggcag gctggcctgc
     5041 tccgacgtgt tccgcaccga ctggcgcgca gcgctcgtca gcggcctgct gtggaccggt
     5101 tcgctcgccg ccggcggcct cgggcggctg acgccgcgct cgctgctcga cagccggccg
     5161 ctgcgcgcgc tgctcggccg gcacatcgac ttcgcggcgg tgcgcacggc gatcgaggac
     5221 ggcacgctgc acgcgttcag cctcagcgcc gccggctaca actccgggca gtcgatcacc
     5281 ttcttcatgg gccatgcggg gctgcggccc tggcagcgca cgcggcgcat cggcatgccg
     5341 cggccgctga cgctcgatca cctgatggcc tcgtgcgcga tcccgttcgt gttcccggcc
     5401 gtgcgcatcg gctacgactg gtacggcgac ggctcgatgc gccagatcgc gccgctgtcg
     5461 gcgccgctgc acctcggcgc cgaccggctg ctggtgatcg gcgtgcgcga ggagaccccg
     5521 gacgaacccg accggccgat cctgcgcctc gaatacccgt cgccgggcca gatcgccggc
     5581 tacatcctcg acacgctgtt catggatgcg ctgtcggccg acatcgagcg cgcgcggcgc
     5641 atcaaccgca cgctggcgct gatcccgccc gagcgccgcg cgcagctggc gctgcggccg
     5701 atcgagatcg ccaccgtgtt cccgagccgc gacgtgcgcc tgatcgccgc gcaccacgaa
     5761 gcgcacttcc cgcgctcggt gcgctacctg ctgcacagcc tcggcgcgac gcgcggacgc
     5821 ggccggccgc tgatcagcta cctgctgttc gaaggcggct tctgcggcga gctgatcgcg
     5881 ctcggctacc gcgacgcaca ggacgcgcgc gagcgcctct gcacgctgct cggcctcgca
     5941 catccggccg ccacgctgcg gccctgaagc ccgccgcacg gcgggccggc cgttcccacg
     6001 cgggcggttt cacgtgaaac cgccccggcc cgtgcgcctg cgccaacttc ggacggatga
     6061 acggatatac tcgcgcgtcc ccggccccgc ccccggtgcg atcaccggcg cggggggctg
     6121 tcgagcgagc ggagaaagcc agcgtgcgcg atgtttcgac gtttcagggc ctgatcctgg
     6181 ccctgcagga atactgggcc gagcagggct gtgcggtgct gcagccctac gacatggaag
     6241 tgggcgccgg caccttccac accgcgacca cgctgcgggc ggtcgggccg gaaccgtggg
     6301 cggcggccta cgtgcagccc tcgcgccgcc cgaccgacgg ccgctacggc gagaacccga
     6361 accggctgca gcactattac cagttccagg tcgtcatcaa gccctcgccg gacgacatcc
     6421 aggacctcta cctcggctcg ctcgcgcgcc tcggcatcga cccgctggtg cacgacatcc
     6481 gcttcgtcga ggacaactgg gaatcgccga cgctcggcgc ctggggcctc ggctgggagg
     6541 tctggctcaa cggcatggaa gtcacgcagt tcacgtactt ccagcaggtc ggcggcctcg
     6601 actgccggcc ggtgaccggc gagatcacct acggtctcga acgcatcgcg atgtatctgc
     6661 agaacgtcga gagcgtctac gacctgacct ggaccgacgg cccgttcgga cgcatcagct
     6721 acggcgacgt gttccaccag aacgaggtcg agcagtcgac ctacaacttc gagcaggccg
     6781 acaccgcctg gctgttccgg cagttcgacc agtgcgaatc cgaatcggcg cggctggtcg
     6841 cgctcgggct ggcgctgccg gcctacgagc agatgctcaa ggcctcgcac accttcaatc
     6901 tgctcgatgc gcgcaaggcg atctcggtga ccgagcgcca gcgcttcatc ctgcgcgtgc
     6961 gcacgctcgc acgcgcctgc gcgcaggcct atcacgatgc ccgcgccgcg ctcggcttcc
     7021 cgatgtgccg gacccccgca gcgatcacgg ccacgaccgg aacgggagcc tgaacatgtc
     7081 gcaggtggag acgaacgatc tgctggtcga gatcggcacc gaggagctgc cgccgaaggc
     7141 cctgccggcg ctctcggaag ccttcgaggc cggcatccgc gacggcctcg ccaaagccgg
     7201 cctgaacttc ggcgccgtgc accgctacgc cgcgccgcgc cgcctcgccg tgctcgtgca
     7261 tgagctgccg atgcagcagc ccgagcgccg gctggagcgc cgcggtccgg cggtgagcgc
     7321 cgccttcggc ccggacggca cgccgacgaa ggccaccgaa ggcttcgcgc gctcctgcgg
     7381 cgtcgaggtc gcggcgctga cgcgcgtggc gaccgacaag ggcgagtggc tcgcgcacgt
     7441 cagcaccgag cccggcgcgc cgctcgcgca gctgctgccc ggcatcgtcg aggccgcgct
     7501 cgcggcgctg ccgatcccca agcgcatgcg ctggggggcg cgcacgcagg aattcgtgcg
     7561 cccggcgcac tggatcgtgc tgctgcacgg cacgcaggtc gtgcccggcg agatcctcgg
     7621 cctgccgacc gggcgcgaga cgcgcggtca ccgcttccac catcccgcgc cgatcgcgat
     7681 cccgaagccg gccgcgtatg cgccgctgct cgccgatcag ggccgcgtgc tcgccgattt
     7741 cgccagccgg cgcgaggcga tccgcaacca ggcggtgcgc gcggcggccg gcctcggcgg
     7801 tgaggcggtg atccgccccg agctgctcga cgaggtcacg gcgctggtcg agtggccggt
     7861 cgtgctggtc ggcagcttcg agccgcgctt cctcgcggtg ccgaacgagg cgctgatctc
     7921 gacgatgcag gacaaccaga agtacttccc ggtcgtcgat gcgcaggggc gcatgctccc
     7981 gcacttcatc acgatcatga acatcgagtc gcgcgacccg gcgcaggtgc gcgccggcaa
     8041 cgagcgcgtg atccggccgc gcttcagcga cgccgagttc ttctggaacc aggaccgcaa
     8101 acgccgcctc gacagccacg tcgagagcct caagaccgtc gtcttccagc agaagctcgg
     8161 cacgctgcac gacaagtccg gacgcgtcgc gcagctggcg cagacgctcg ccggcgtgct
     8221 cggcgccgat gccgcgctcg ccgcccgcgc cgcgcagctc gccaagtgcg acctgatgac
     8281 caacatggtc ggcgagttcc cggagctgca gggcacgatg ggccgctact acgccgccca
     8341 cgacggcgaa gcggccgaac tcgcgcaggc gctggaggaa cagtaccggc cgcgcttcgc
     8401 cggcgacgcc atcgccacca gcgccaccgg ccgcatcgtc gcgcttgccg atcgcctcga
     8461 cacgctggtc ggcatcttcg cgatcggcca gccgccctcg ggcgcgaagg acccgttcgc
     8521 gctgcgccgc gccgcgctcg gggcgctgcg cacgatcatc gagggcgggc tggaaatcga
     8581 cctgcacgcg acgctgaccg gggccgccgc ggccttcccg gcggcgctcg cggcgccggc
     8641 agccgtcgat gcggtgttcg acttcatgat cgagcggctg cgcgcgttct acaccgaacg
     8701 cgtcggcgag ccggtgcgac ccgacgagtt cgatgccgtt gccgcctgcc gcccgacccg
     8761 gccgctcgac ttcgaccgcc ggctgcgcgc gctcgcggcc ttccgccg
//
LOCUS       DAOQIE010000157         8801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4041_length_8801_cov_11.127144, whole genome shotgun sequence.
ACCESSION   DAOQIE010000157 DAOQIE010000000
VERSION     DAOQIE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4041_length_8801_cov_11.127144"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..987)
                     /locus_tag="PK144_10695"
     CDS             complement(7..987)
                     /locus_tag="PK144_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF66240.1"
                     /translation="MAADPQTASPIDPNVERQTRVFLEALLAAGGPPLETLAPKDARA
                     VLTGAQASVDLKLPPAEVSERTIEQDGHRVRLHIVRPAGVSGVTPAFLFIHGGGWILG
                     DFPTHERLVRDLVAGSGATAIFVNYTPSPEARFPVAIEENHAAAKWVAAHAEELGIDA
                     QRLGVAGNSVGGNMSTVLCLMAKDRGGPALRCQILLWPVTNADFSSASYQQFAEAHFL
                     TQAMMRWFWDAYTTDPAERAHRYASPLRASDAELAGLPPALIQTAECDVLRDEGEAYA
                     RRLDAAGVEVTLTRHGGLIHDYGLLNPVAHIPAVQTAVRQAAAQLKHYLG"
     gene            complement(1191..2783)
                     /locus_tag="PK144_10700"
     CDS             complement(1191..2783)
                     /locus_tag="PK144_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66241.1"
                     /translation="MRHRTFRWLWLAAIASNIGTWMHEVGAGWLMTTLSPSPLLVSLV
                     QAAGAAPMFVLALPAGALSDIVDRRRFLIGVQIWMALVAAALAAATLTGAMTPGLLLG
                     LTAALGIGSALMAPAWAALTPELVPKPELQSAIALSSVGINVARAIGPALAGLIVSSV
                     GPWATFALNAASFAGVIVVLAGWRRPPAVSTLPSERFFGALRAGWRYARSAPRLQAVI
                     VRALAFFVFASAGLGLLPLLVRRLPDGSAGVYGALLACVGVGAVGGAFLLPKLRERVS
                     ADQLVAGASALYAGVLLALALLPSAPWLAPVMLASGAAWIAVLSSFQVAAQTAAPPWV
                     RARALAVYMLAFNGAMAAGAALWGTIAAHQAPAVALVAAAAGLLLLILPVRRFRLACA
                     AADDLSPSLHWPMPLAEGTAIEHDRGPVLVTVEYEIPPAHAAAFVQAMQAMARVRRRN
                     GALSWGLFQDTDRPQRWVEYFIDESWLEHLRHHGRITHADAEIEARVRALHSGPHGPR
                     IHHHVGPPAPGSGLLPLGAGEG"
     assembly_gap    2827..2926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2972..4858)
                     /locus_tag="PK144_10705"
     CDS             complement(2972..4858)
                     /locus_tag="PK144_10705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF66242.1"
                     /translation="MSADASAAPSANSGSGAELILLNGRFHTVDRERPDASAVAIRAG
                     RFVAVGDAAEVMRWQGPASVVVSLDGHCAIPGLIDSHLHLIRGGLNYNLELRWEGLPS
                     LADALALLRRQAQRTPPGQWLRVVGGWTEFQFAEKRLPTLAELNAAAPETPVFVLHLY
                     DRALLNRAALRAVGYTRDTPEPPGGAIERDAAGEPTGLLLAKPNAMILYATLAKGPKL
                     PLEQQANSTRHFMRELNRLGVTSAIDAGGGFQNYPDDYAVIDRLAADGELTVRIAYNL
                     FTQRPGQELDDFTHWTETVPLRQGDDWYRHNGAGEMLAFSAADFEDFVMPRPELPVRM
                     ETELEAVVRRLVAGRWPFRLHATYDESITRMLDVFERVDRDLPFAGLPWFFDHAETVS
                     PRNLERIRALGGGIAIQHRMAFQGEYFVERYGAEAARATPPVARMLELGIPVGAGTDA
                     TRVASYNPWTALYWLVSGRTVGGLALAPHGLARDTALELWTAGSAWFSSDQGNKGRIR
                     VGELADLAVLSADYFSVPEEDIRAIESLLTVVGGRIVHAAGGFAPLAPPPLPVLPEWS
                     PVAIAPGHHRPAPPAAALPHQCLGACAVHGHAHDRARRAAVPVAAGELQGFWGAFGCS
                     CFAF"
     gene            complement(4919..5539)
                     /locus_tag="PK144_10710"
     CDS             complement(4919..5539)
                     /locus_tag="PK144_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNF66243.1"
                     /translation="MNAAAPAAAAPEPPEPSEPPAAVFGGAHAGHVSFVIRHRVEPRR
                     RADYERWLTRIMPVAATYPGHLGVSVIRPDAPGTPYTIVLRFERLEQLRRWMESADRR
                     ELIEQIRPLLAAGEDLDIRPGAAFWFTPAGAQPPRLWKQVLMTFAVIWPLTLIVPQLW
                     QPAFTRWPWLGGWLAGNFVVALSIVLIVVCAVMPWLTRRLAGWLTR"
     gene            complement(5536..6207)
                     /locus_tag="PK144_10715"
     CDS             complement(5536..6207)
                     /locus_tag="PK144_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009923512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNF66244.1"
                     /translation="MTDRTALEQLTPDNSVLLFIDHQPQMAFGVANIDRQTLKNNVVA
                     LAKSAKVFGVPTFITTVETESFSGYTWPELLDVYPDAALLERTSMNSWDDAKVRDAIN
                     GSGRRKLILSGLWTEVCINSAANSAMLDGYEVYVVADACGGTSKDAHDYSMLRMIQAG
                     IRPLTWQQTLLEWQRDWARREHYDEVMAIVREHSGAYGMGVDYAYTRVHGAQQRGQWR
                     GSVRK"
     gene            6446..8146
                     /locus_tag="PK144_10720"
     CDS             6446..8146
                     /locus_tag="PK144_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF66245.1"
                     /translation="MTDPSPPNGPAFAALRADIEHLLQRFTLGEHGGRMLLQGRRMVV
                     QSARSLGQLREELIRMLGADLARGVLKRYGYQSGFQDGHALRLRHPELPVELQMHLGT
                     VLHEREGHARIETEPEGTHVDLARGVLDIRSRWHDSVEAEQHHLLLGRSSEPVCWTLS
                     GYASGHLSYLLQREVVIVETACRAQGHPYCRFRVGFREDMEKHFPGCADDYRRLDLGR
                     LFRELEQTVEAQSRTIRELREQLGAAPAAGAAASAAFSTLTGRSPALARAIDIARAVA
                     GVSSTVLVTGESGTGKELLARGIHAASARAAGPWIALNCATLTESLQIAELFGHAKGA
                     YTGAAGERAGLFEAASGGTLFLDEVGELSPSAQAALLRVLQEGVIVRVGEHRERPVDV
                     RIVAATHRDLEARVGAGQFRADLFYRLNVVSIRMPALRERDNDVLLLAQSLLERYGAR
                     FGRNVQALSPEVLRLFLAHPWPGNVRELANVLERAVLLARGTRIELEDLPDGFAAAVH
                     GDGAAAALPGAPEVIGQQERERIRAALERSHGRRDVAATLLGISRATLWRRMKRLGLA
                     "
     gene            8207..8653
                     /locus_tag="PK144_10725"
     CDS             8207..8653
                     /locus_tag="PK144_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF66246.1"
                     /translation="MSEQTVRLHRVLRAPPGRVYRAFLDADALCRWLPPYGFLCRVHE
                     LDAQVGGHFRMSFRNFATGREHFFGGEYLELQPEQRIRYVDRFDDPNLPGLMNVTIRL
                     RAVLCGTDLQIEQGGLPEAIPVEMCYLGWQESLVQLASLVEPEIAD"
BASE COUNT         1235 a         3090 c         3199 g         1177 t
ORIGIN      
        1 ccggcctcag cccagataat gcttcagctg cgccgccgcc tggcgcaccg ccgtctgcac
       61 ggccggaatg tgcgcgaccg ggttcagcag gccgtagtcg tggatcaggc cgccgtggcg
      121 ggtcagcgtc acctcgacgc cggcggcatc gaggcggcgg gcgtaggcct cgccctcgtc
      181 gcgcagcacg tcgcactcgg cggtctggat cagcgccggc ggcaggccgg cgagttcggc
      241 gtcgctggcg cgcagcggcg aggcgtagcg gtgggcgcgc tcggccggat cggtcgtgta
      301 ggcgtcccag aaccagcgca tcatcgcctg cgtcaggaag tgcgcctcgg cgaactgctg
      361 gtagctcgcg ctcgagaaat cggcgttggt caccggccag agcaggatct ggcagcgcag
      421 cgccggaccg ccgcgatcct tcgccatcag gcagagcacc gtcgacatgt tgccgccgac
      481 gctgttgccg gccaccccga ggcgctgcgc gtcgatgccg agctcttcgg catgcgccgc
      541 gacccacttc gccgccgcgt ggttctcctc gatcgccacc ggaaagcgcg cctcgggcga
      601 gggcgtgtag ttgacgaaga tcgccgtcgc gccggagccg gcgaccagat cgcgcaccag
      661 gcgctcgtgg gtcgggaagt caccgaggat ccagccgccg ccgtggatga acaggaaggc
      721 cggcgtcacg ccgctcacgc cggccgggcg cacgatgtgc aggcgcacgc ggtgaccgtc
      781 ctgctcgatc gtgcgctcgc tgacctcggc cggcggcagc ttcaggtcga cgctcgcctg
      841 cgcgccggtc agcacggcgc gcgcatcctt cggtgccagg gtttccagcg gtggcccgcc
      901 ggccgcgagc agggcttcga gaaagacgcg ggtctggcgt tcgacgttcg ggtcgatggg
      961 cgaagcggtc tgcggatcgg cggccatgga gggttcctcg gcggggcttc ggaaagaacg
     1021 accgtccggc cacgcggggg cgtgtggacg cacggtcgcg gtgagcgcac tcggcgctgg
     1081 aatcgaattg cttgcaaact gaaagcctat gaagaagccg gtgctcccgg catgaacatc
     1141 cggcaagccg ggtgcggggg gcgttgctgc agcagtcccg gttttcgggc tcagccttcg
     1201 ccggctccga gcggcagcag gccgctgccc ggggccggcg ggccgacgtg gtgatggatg
     1261 cgcgggccgt gcggccccga gtgcagcgcc cgcacgcgcg cctcgatttc tgcatcggcg
     1321 tgcgtgatgc ggccgtggtg gcgcaggtgt tcgagccagg attcgtcgat gaagtactcg
     1381 acccagcgct gcggccggtc ggtgtcctgg aacaggcccc agctcagcgc gccgttgcgc
     1441 cggcgcacgc gcgccatcgc ctgcatcgcc tgcacgaaag cggccgcgtg cgccggcgga
     1501 atctcgtatt cgaccgtcac cagcaccggc ccgcggtcgt gctcgatggc cgtgccttcg
     1561 gccagcggca tcggccagtg cagcgacggc gacaggtcat cggccgccgc gcaggcgagc
     1621 cggaagcggc gcaccggcag gatcagcagc agcagcccgg ccgcggcggc gacgagcgcc
     1681 acggccggcg cctggtgcgc ggcgatcgtg ccccagagcg ccgcgccggc ggccatcgcg
     1741 ccgttgaacg cgagcatgta gacggcgagc gcgcgtgccc gcacccacgg cggcgcggcg
     1801 gtctgcgcgg cgacctggaa cgaggacagc acggcgatcc aggccgcacc gctggccagc
     1861 atcaccggcg ccagccacgg cgccgacggc agcagcgcca gcgcgagcag cacgccggcg
     1921 tacagcgcgc tcgccccggc cacgagctgg tcggccgaca cgcgctcgcg cagcttcggc
     1981 agcaggaagg cgccgccgac cgcgccgacg ccgacgcagg cgagcagcgc gccgtagacg
     2041 ccggcgctgc cgtccggcag ccgccgcacc agcagcggca gcaggccgag cccggcgctc
     2101 gcgaacacga agaaggccag cgcgcgcacg atcaccgcct gcagccgcgg ggcgctgcgc
     2161 gcgtagcgcc agccggcccg cagcgccccg aagaagcgct cggacggcag cgtcgatacc
     2221 gccggcggcc ggcgccagcc ggcgagcacg acgatgacgc cggcaaacga cgcggcgttc
     2281 agcgcgaagg tcgcccacgg cccgacgctg ctgacgatca gcccggccag cgccggcccg
     2341 atcgcgcgcg cgacgttgat gccgacgctg gagagcgcga tcgccgattg cagctcgggc
     2401 ttcggcacca gctccggcgt cagcgccgcc caggccggcg ccatcagcgc cgagccgatg
     2461 ccgagtgccg cggtcagccc gagcagcagc cccggcgtca tcgcgccggt gagcgtcgcc
     2521 gccgcgagtg cggcggcgac cagcgccatc cagatctgca cgccgatcag gaagcgccgg
     2581 cgatcgacga tgtcggacag cgcgccggcc ggcagcgcca gcacgaacat cggcgccgcc
     2641 ccggcggcct gcaccagcga caccagcagc gggctcggcg acagcgtcgt catcagccag
     2701 ccggcgccga cctcgtgcat ccaggtgccg atgttcgacg cgatcgccgc cagccacagc
     2761 cagcggaagg tgcgatggcg cagcggactc caggcgctgg ccggcgcccc ggccggcggc
     2821 gggaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatc ggatcgtttc
     2941 cttcaggccg gcggcggcgg tgcgcgccgc ttcagaacgc gaagcacgag cagccgaacg
     3001 cgccccagaa gccctgcagc tcgccggcgg cgaccgggac tgcggcgcgg cgggcgcggt
     3061 cgtgggcgtg gccgtgcacg gcgcaggcac ccaggcactg gtgcggcagc gcggcggcgg
     3121 gcggagcggg gcggtgatgg cctggcgcga tcgcgaccgg ggaccattcc ggcagcaccg
     3181 gcagcggcgg cggcgccagc ggggcgaagc cgcccgcggc gtgcacgatg cggccgccga
     3241 cgacggtcag cagcgattcg atcgcgcgga tgtcctcttc cggcaccgag aagtaatcgg
     3301 ccgacagcac ggccaggtcg gccagctcgc cgacgcggat gcggcccttg ttgccctgat
     3361 ccgacgagaa ccaggcgctg ccggccgtcc agagttcgag cgcggtatcg cgcgcgagac
     3421 cgtgcggggc cagcgccagg ccgccgaccg tgcggccgct gaccagccag tacagcgccg
     3481 tccacgggtt gtagctcgcc acgcgcgtgg catcggtgcc ggcgccgacc gggatgccga
     3541 gttcgagcat gcgcgcgacc ggcggcgtcg cgcgcgcggc ctcggcgccg tagcgctcga
     3601 cgaagtactc gccctggaag gccatgcggt gctggatggc gatgccgccg ccgagcgcgc
     3661 ggatgcgttc gaggttgcgc gggctgaccg tctcggcgtg gtcgaagaac cacggcaggc
     3721 cggcgaacgg caggtcgcga tcgacgcgct cgaacacgtc gagcatgcgc gtgatcgatt
     3781 cgtcgtaggt cgcgtgcagc cggaacggcc agcgtccggc gacgagccgg cgcacgacgg
     3841 cttcgagctc ggtctccatg cgcaccggca gctccgggcg cggcatcacg aagtcctcga
     3901 agtcggcggc cgagaacgcc agcatctcgc cggcgccgtt gtgccggtac cagtcatcgc
     3961 cctggcgcag cggcacggtt tcggtccagt gcgtgaagtc gtcgagctcc tgcccggggc
     4021 gctgcgtgaa caggttgtag gcgatgcgca cggtcagctc gccgtcggcg gcgaggcggt
     4081 cgatcaccgc gtagtcgtcc gggtagttct ggaagccgcc gccggcgtcg atggcgctgg
     4141 tgacgccgag gcggttcagc tcgcgcatga agtggcgcgt cgagttggcc tgctgctcca
     4201 gcggcagctt cgggcctttc gccagcgtcg cgtacaggat catcgcgttc ggcttcgcga
     4261 gcagcaggcc ggtcggctcg ccggcggcgt cgcgctcgat cgcgccgccg ggcggctcgg
     4321 gcgtgtccct ggtgtagccg acggcgcgca gcgcggcgcg gttgagcagt gcgcggtcgt
     4381 acaggtgcag cacgaacacc ggcgtttccg gtgccgcggc gttcagctcg gcgagcgtcg
     4441 gcaggcgctt ctcggcgaac tggaattcgg tccagccgcc gaccacgcgc agccactgcc
     4501 cgggcggcgt gcgctgcgcc tggcggcgca gcagcgccag cgcatcggcg agcgagggca
     4561 ggccctccca gcgcagttcg aggttgtagt tcaggccgcc gcggatcagg tgcaggtgcg
     4621 agtcgatcag gccgggaatc gcgcagtggc catcgaggct gacgacgacg ctggccggcc
     4681 cctgccagcg catcacctcg gcagcatcgc cgaccgcgac gaagcgcccg gcgcggatgg
     4741 cgacggcgct cgcgtccggg cgctcgcgat cgacggtatg gaagcggccg ttcagcagga
     4801 tcagctcggc gccggagccg gagttggcgg agggagcagc ggatgcgtcg gcggacatgc
     4861 gcggatacct cggggggagc ggggggagag ccgccgcgca cggtgcggcg gcggggattc
     4921 agcgcgtcag ccagccggcg agccggcgcg tcagccacgg catcaccgcg cagacgacga
     4981 tcagcacgat gctgagcgcg acgacgaaat tgccggccag ccagccgccg agccacggcc
     5041 agcgcgtgaa cgccggctgc cagagctgcg gcacgatcag cgtcagcggc cagatcaccg
     5101 cgaaggtcat cagcacctgc ttccagagcc gcggcggctg cgcgccggcc ggcgtgaacc
     5161 agaaggccgc accggggcgg atgtccaggt cctcgccggc ggcgagcagc ggccggatct
     5221 gctcgatcag ttcgcgccgg tcggccgatt ccatccagcg gcgcagctgc tccaggcgct
     5281 cgaagcgcag cacgatcgtg tacggcgtgc cgggcgcatc cgggcggatg acgctgacgc
     5341 cgagatggcc cggatacgtg gccgccaccg gcatgatccg cgtcagccag cgctcgtagt
     5401 ccgcgcgccg gcggggttcg acgcggtggc ggatcacgaa gctgacgtgg ccggcgtggg
     5461 caccgccgaa caccgcggcg ggcggttcgg aaggctcggg cggctcgggc gctgcggctg
     5521 ccggtgcggc ggcgttcact tgcgcacgct gccgcgccac tggccgcgct gctgtgcgcc
     5581 gtgtacgcgc gtgtaggcgt agtcgacacc catgccgtag gcgccgctgt gctcgcgcac
     5641 gatggccatg acctcgtcgt agtgctcgcg ccgcgcccag tcgcgctgcc attcgagcag
     5701 ggtctgctgc caggtcagcg gccggatgcc ggcctggatc atgcgcagca tcgagtagtc
     5761 gtgcgcgtcc ttgctggtgc cgccgcaggc atcggcgacg acgtagacct cgtagccgtc
     5821 gagcatcgcc gagttggcgg ccgagttgat gcagacctcg gtccagaggc cggagaggat
     5881 cagcttccgg cggccgctgc cattgatcgc gtcgcgcacc ttggcatcgt cccaggaatt
     5941 catgctggtg cgctccagca gggctgcgtc cggatagacg tcgagcagct ccggccacgt
     6001 gtagccgctg aaggattcgg tctcgaccgt ggtgatgaag gtcggcacgc cgaacacctt
     6061 cgccgacttg gcgagggcga cgacgttgtt cttcagcgtc tggcgatcga tgttggcgac
     6121 gccgaaggcc atctgcggct ggtgatcgat gaacagcagc acgctgttgt cgggcgtcag
     6181 ctgttcgagc gcggtacggt cggtcatggg tcggctcctg cggaggggat gagctttctt
     6241 tcgaagaccc ggccgggcga gccagcctgc gcaggtttcg ggccagttcg cacaaggtgg
     6301 aaaaaaccga gtgttacact cggaatgtga acaggcgttc atgcacggtt gcagcctgcg
     6361 ttgcagacac gtttcatggt tgcgctgtca tgaaacatcc gctgcgacat ccccctgatt
     6421 ttcctgcggc tgaaaccctt ccccgatgac cgatccctct cccccgaacg gcccggcctt
     6481 cgccgcgctg cgtgccgaca tcgagcatct gctgcagcgc ttcacgctcg gcgagcacgg
     6541 cggacgcatg ctgctgcagg gccggcgcat ggtcgtgcag tcggcgcgct cgctcgggca
     6601 gctgcgcgag gagctgatcc gcatgctcgg cgccgatctg gcgcgcggcg tgctgaaacg
     6661 ctacggctac cagtccggct tccaggacgg ccacgcgctg cgcctgcgcc atccggagct
     6721 gccggtcgag ctgcagatgc acctcggcac cgtgctgcac gagcgcgagg gccacgcgcg
     6781 catcgagacc gagcccgagg gcacgcacgt cgatctggcg cgcggcgtgc tcgacatccg
     6841 ctcgcgctgg cacgactcgg tcgaggccga gcagcaccac ctgctgctcg ggcgcagcag
     6901 cgaaccggtg tgctggacgc tctccggcta tgccagcgga cacctgagct acctgctgca
     6961 gcgcgaggtc gtgatcgtcg agaccgcctg ccgcgcccag ggccatccgt actgccgctt
     7021 tcgcgtcggc tttcgcgagg acatggaaaa acacttcccc ggctgcgcgg atgactatcg
     7081 ccgactcgac ctcggccggc tgttccgcga gctggagcag accgtcgagg cgcagagccg
     7141 cacgatccgc gaactgcgcg agcagctcgg cgccgcgccg gccgccggcg cggcggcctc
     7201 ggcggcgttc tcgacgctga ccggccgcag ccccgcgctc gcgcgcgcca tcgacatcgc
     7261 gcgcgcggtc gccggcgtca gctcgacggt gctggtgacc ggcgagagcg gcaccggcaa
     7321 ggagctgctg gcgcgcggca tccacgccgc ctcggcgcgc gccgccggac cgtggatcgc
     7381 gctcaactgc gcgacgctga ccgaaagcct gcagatcgcc gagctgttcg gccacgcgaa
     7441 gggcgcctat accggcgccg ccggcgagcg cgccggcctg ttcgaggccg cgagcggcgg
     7501 cacgctgttc ctcgacgagg tcggcgagct gagcccctcg gcgcaggccg cgctgctgcg
     7561 cgtgctgcag gaaggcgtga tcgtgcgcgt cggcgagcac cgcgagcggc cggtcgacgt
     7621 gcgcatcgtc gccgccacgc accgcgacct cgaagcgcgg gtcggggccg ggcagttccg
     7681 cgccgatctc ttctaccggc tcaacgtcgt cagcatccgc atgccggcgc tgcgcgagcg
     7741 cgacaacgac gtgctgctgc tcgcgcagtc gctgctcgaa cgctacggcg cgcgcttcgg
     7801 gcgcaacgtg caggcgctgt cgccggaggt gctgcggctg ttcctcgcgc atccgtggcc
     7861 cggcaacgtc cgcgaactcg ccaacgtgct ggagcgcgcc gtgctgctcg cacgcggcac
     7921 gcgcatcgag ctggaggacc tgcccgatgg cttcgccgct gccgtgcacg gcgacggcgc
     7981 tgccgcggcg ctgccgggcg cgcccgaagt catcgggcag caggaacgcg agcgtatccg
     8041 cgccgcgctc gagcgcagcc acggccggcg cgatgtcgcg gccacgctgc tcggcatcag
     8101 ccgcgcgacg ctgtggcggc gcatgaagcg cctcggactg gcctgagggc cgtcagccgc
     8161 tacgctgacg caggtttccg ccgctcccgc cgaggtcgca gccgtcatgt ccgagcaaac
     8221 cgtccgcctg caccgcgtgt tgcgcgcccc gcccgggcgg gtctaccgcg ccttcctcga
     8281 tgccgatgcg ctgtgccgct ggctgccgcc ctacggcttc ctgtgccgcg tgcacgagct
     8341 cgacgcgcag gtcggcgggc acttccgcat gtcgttccgc aacttcgcga ccggacgcga
     8401 acacttcttc ggcggcgaat acctcgaact gcagccggag cagcggatcc gctacgtcga
     8461 tcgcttcgat gacccgaacc tgcccggcct gatgaacgtg acgatccggc tgcgcgcggt
     8521 gctctgcggc accgatctgc agatcgagca gggcggcctg cccgaagcca tcccggtgga
     8581 gatgtgctac ctcggctggc aggaatcgct cgtgcagctc gccagcctgg tcgagccgga
     8641 gattgcggac tgataccgcc cacgccggaa cgctcgcctt ttttcagccc cccggccctc
     8701 gtgggagagg gaccggacca gactgatctg gcagggcttt ccgcagcggc gcttcagacg
     8761 acctttttgt ccccgtccca ggtcacgaag ggtttgtccg c
//
LOCUS       DAOQIE010000158         8800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4043_length_8800_cov_8.028702, whole genome shotgun sequence.
ACCESSION   DAOQIE010000158 DAOQIE010000000
VERSION     DAOQIE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_8800_cov_8.028702"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PK144_10730"
     CDS             complement(<1..1048)
                     /locus_tag="PK144_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66247.1"
                     /translation="MSTPPVAITGIGLVSALGLDAVVACAAARAGLNRLRALDVLQWS
                     AAEVFGGEPVAGHAVPLVASGFVGFAKTLALAHHALADLFARTPLAGAEAAHFGMVLL
                     VSDGFLEDRLHDPEAEEDLPPPSQRWRAEAARLVPLLARLAGLPPARCRIVHGHHAEF
                     AEALALGAEAIARQRLQGCLVGALDCAFEPQRLIAAAEAELLKTAGNPVGHLPGEAGG
                     FVLLEAAGRSGGGRKALARLGGLERRRTRAWDPEAAAPDGSELAAALIGALPPAPGPG
                     WFVGDLNGEAARAQEWGEVLVRVQLARAGAADWPLELPALAFGETGAAAGVVGKAVAL
                     HQFARRCAPPGSALL"
     gene            complement(1079..1540)
                     /locus_tag="PK144_10735"
     CDS             complement(1079..1540)
                     /locus_tag="PK144_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HNF66248.1"
                     /translation="MPVTVGVNMLSVVHAGSNGITTAFPDVCKTPAPPAPPIPIPYPN
                     IAQSSDSASTASSVTADGNPICVKDSNFRMSTGDEPGSLLGVASNKIKGKAEFVNFSF
                     DVQAEGKNVCRALDLMLHNDKNTPPFPVLQGPIIALPSDEDVVCWCCGESF"
     gene            complement(1625..2077)
                     /locus_tag="PK144_10740"
     CDS             complement(1625..2077)
                     /locus_tag="PK144_10740"
                     /inference="COORDINATES: protein motif:HMM:NF040117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HNF66249.1"
                     /translation="MKQITLRIPAAPAPVPAGSGGTEGLWSGIDAEGRPLVDWPGNAH
                     GAPQPARFLAHVLDGQRPGHAPLAVWLDVSGAQPLILGVLATRLAPADVALDGERVVL
                     EAACELELRCGPARIVLTRDGEVIVRGVNITSRAAASHRIRGASVQIN"
     gene            complement(2080..4254)
                     /gene="tssI"
                     /locus_tag="PK144_10745"
     CDS             complement(2080..4254)
                     /gene="tssI"
                     /locus_tag="PK144_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNF66250.1"
                     /translation="MPITQANRPVTVSCSLGTDKLLLRRLHGRERLGRLFEYELELAS
                     EDFAIDPAGLLGKPLGVELELPSGDGSRWFHGIVASFGQSGSDGRIATYRVRLRPWFW
                     LLTHCSDCRIFQAKSVPDIVKQVFENLGFADYELKLNGSYAPREYCVQYRETDFDFVS
                     RLLEKEGIFYYFRHEDGRHVLVLVDDNASLAAQPGYEEVPYFPPDQLGRRPRDHLWAW
                     SQALSVQTSALTLNDFDFERPSAQLLAASSASGPAAGYKRYDYPGAYTQTSPGEHYAR
                     TQLEALRTRAAECRFTGDARGLSCGALFTLSGCPNDKLNIELLITSAEYRLTQDAYHS
                     GPNAGKLDFELDIAAQDNQLPFRSEPLTPPAQVLGPQTAIVVGPAGEEIFTDKYGRVK
                     VHFHWDRYDGQDEHSSCWIRVAQVWAGKGWGGMMIPRIGQEVVVDFLEGDPDRPLITG
                     RVYNGEQPVPFALPANATQSGWRSRSSKGGSTANCNEIRMEDLKGSEQLFIHAEKDEL
                     HEVENDQTLSVGHDRSETTGRDRTLVVGRDKSETVDRHKTVHIVGNHVERIDMNMEVS
                     VASNLTETVGINYSETVGAAMELTVGGLLNIAVGAAMSEEVGLQKTDVVGGAHSVTIG
                     GNRSIEVGGAVTETIDKDQTNKIGKNLTTEVGENCKETVKKDYSLQAKRIQLVADDEI
                     ALKTGSAELTLKKDGTILIKGAKITIKGSGDVVIKGSKIAEN"
     gene            complement(4276..5634)
                     /locus_tag="PK144_10750"
     CDS             complement(4276..5634)
                     /locus_tag="PK144_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF66251.1"
                     /translation="MYPDSGEDPPTEAVTLEQALALAVGWQREGRLDAAEPVYRQILA
                     VLPDQPDALHFLGLLEFQRGHEDAALEWLARAVAVAPGHAGLRSNHGNVLQTLGRLDE
                     AEREYRAALAAEPALVAAWNNLGVVLHAAGRRHEAIEAYRHALELEASSAADHHRLAT
                     LLERCHEYEAAAVHHRAALALDPQHLGATRALGHALSRLGRLDEAAAAYRAWLALEPG
                     HPLAELRLAACGGAPAPARCSDAVVRGLFDQYAGHFDAHLRGTLDYHAPERLADALAL
                     VLDAPAAALDVLDAGCGTGLCGPLLRPYARQLSGVDLSPKMLEQAASLALYDALETAE
                     LCAWLNAHPGAFDLLVAADTLCYFGPLADFAAAAAAALRPGGWLAFTLETHTEALDHR
                     LNACGRYSHAPDYPLRVLAGAGFGQLRSQPVVLRRERGAPVEGLVVLARRPATPGYAC
                     AD"
     gene            5815..6762
                     /locus_tag="PK144_10755"
     CDS             5815..6762
                     /locus_tag="PK144_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF66252.1"
                     /translation="MHPPALRIRQLVKTYRSGVTALHGIDLDVPDGDFFALLGPNGAG
                     KSTTIGIVSSLVNKTAGRVEIFGHDLEAQAWAAKRLIGLVPQEFNFNQFEPVIEIVVN
                     QAGYYGIPRHVARPRAEELLERLGLAAKARDMARELSGGMKRRLMIARALIHAPRLLI
                     LDEPTAGVDIELRRSMWEFLRETNAAGTTIILTTHYLEEAESLCRNIAIIDRGRLVEH
                     TDMKSLLARLHTETFVLNLAAPLVALPLLPGYVLDRVDDTTLEVEVSKETGMNALFAG
                     LAAHGIEVLSLRNKSNRLEELFIRLVESGAEARAAGTPA"
     gene            6759..7592
                     /locus_tag="PK144_10760"
     CDS             6759..7592
                     /locus_tag="PK144_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012128865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66253.1"
                     /translation="MKPTPPDILAPALGAGEAPPSEFAGWWTAFVTLLIKEVLRFARI
                     WIQTILPPAVTTALYFVIFGKLLGAQLPPMDGIGYPQYIAPGLIMLAIITNSYANVVS
                     SFYGSKFQHNIEELLVAPMPNWLILAGFVGGGVLRGLAVGVVVTMIALTFTDLHVTHW
                     GVTVGVVFLTAVLFSLAGLINGIYANSFDDIAIVPTFVLTPLTYLGGIFYSINLLPDF
                     WQNVSLVNPILYMVNAFRYGILGVSDIGMLQAFSLIGAFVLGLGAFALWLLKRGVGIR
                     N"
     gene            complement(7620..8438)
                     /locus_tag="PK144_10765"
     CDS             complement(7620..8438)
                     /locus_tag="PK144_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNF66254.1"
                     /translation="MTARSLRPDAGQPGMLRVLCLLLAGLLLLAGCDREDAQARARAA
                     DPVPSNLPATPKSFETAKRQLLGEVYRDRRVDFYCGCRFDADKQVDAAGCGLEARSNA
                     SRATRIEIEHVFPAAQFGNFRQCWREPREFAECTTAGGRRLGGRECCEKVDPVFRAAH
                     NDLHNLYPVVGEVNGDRQDYNWGMLAGEQRAYGRCDFEIDAGERRVEPPARIRGDIAR
                     TMLYMAQTYGFRLSDQDRKLYEAWNRQDPPDSFERERNRRIRALQGNANPFVGD"
     gene            complement(8568..>8800)
                     /locus_tag="PK144_10770"
     CDS             complement(8568..>8800)
                     /locus_tag="PK144_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNF66255.1"
                     /translation="GRQKGHTELYRGAEYVVDFLPKVKVEIACEQSNVERVIDAIARA
                     ANTGKIGDGKLFVFNLEQAIRIRTGETGSEAL"
BASE COUNT         1255 a         3067 c         3139 g         1339 t
ORIGIN      
        1 ccagcagcgc gctgcccggc ggcgcgcagc ggcgggcgaa ctggtgcagc gcgacggcct
       61 tgccgaccac gccggccgcc gcgccggttt cgccgaaggc gagcgccggc agctccagcg
      121 gccagtcggc ggccccggcg cgcgcgagct gcacgcgcac cagcacctcg ccccactcct
      181 gcgcgcgcgc ggcctcgccg ttgagatcgc cgacgaacca gcccgggccc ggggccggcg
      241 gcagcgcgcc gatcagcgcg gccgcgagtt cactgccatc gggcgccgcg gcctcggggt
      301 cccaggcccg cgtgcgccgg cgttcgaggc cgccgaggcg cgcgagcgcc ttccgcccgc
      361 cgccggagcg gccggcagct tcgagcagca cgaacccgcc ggcctcgccg ggcaggtgac
      421 cgaccgggtt gccggcggtc ttcagcagct cggcttcggc ggcggcgatc aggcgctgcg
      481 gctcgaacgc gcagtcgagc gcgccgacca gacagccctg cagccgctgc cgggcgattg
      541 cctcggcacc gagcgccagc gcctcggcga actcggcatg gtggccgtgc acgatgcggc
      601 agcgcgccgg cggcaggccg gcgaggcgcg ccagcagcgg caccagccgc gccgcctcgg
      661 cgcgccagcg ctgcgagggc ggcggcaggt cctcctcggc ctcgggatcg tgcaggcggt
      721 cttcgagaaa gccgtcggac accagcagca ccatcccgaa gtgcgcggcc tcggcgccgg
      781 cgagcggggt gcgggcgaac aggtcggcga gcgcgtgatg ggcgagcgcg agcgtcttgg
      841 cgaagccgac gaaaccgctg gcgaccagcg gcacggcgtg gccggcgacc ggctcgccgc
      901 cgaagacctc ggccgcgctc cactgcagca cgtcgagcgc gcgcaggcgg ttgagaccgg
      961 cgcgcgccgc ggcacaggcg acgacggcgt cgaggccgag tgccgacacc aggccgatgc
     1021 cggtgatggc gacgggaggc gtgctcatcg gaggctcatc gacaagggtt cgacggcttc
     1081 agaaactttc gccgcagcac cagcagacca cgtcctcgtc cgacggcagc gcgatgatcg
     1141 gcccctgcag caccgggaag ggcggcgtgt tcttgtcgtt gtgcagcatc aggtcgagcg
     1201 cgcggcagac gttcttgccc tcggcctgca cgtcgaacga gaaattcacg aactcggcct
     1261 tgcccttgat cttgttcgat gcgacgccga gcaggctgcc gggctcatcg ccggtgctca
     1321 tgcggaagtt cgaatccttg acgcagatcg gattgccgtc ggcggtgacg ctgctcgcgg
     1381 tcgaggccga atcgctcgac tgcgcgatgt tcgggtacgg gatcggaatc ggcggcgccg
     1441 gcggggccgg cgtcttgcag acatccggaa acgcggtcgt gatgccgttg ctgccggcat
     1501 gcacgaccga aagcatgttg acgccgacgg tcacgggcat cgcgaaaacc ccgggtgggc
     1561 aaggaagcgg tgaaggatcg gaggagggcc ggtggaggtg cgcgggtggc ggtccgcccg
     1621 gccgtcagtt gatctgcacc gacgcgccgc ggatgcggtg gctggcggcg gcgcggctgg
     1681 tgatgttgac gccgcgcacg atgacctcgc cgtcgcgcgt gagcacgatg cgcgccggcc
     1741 cgcagcgcag ttcgagttcg cacgcggctt cgagcacgac gcgctcgccg tcgagcgcga
     1801 catcggccgg cgcgagccgc gtggcgagga cgccgaggat cagcggctgc gcgcccgaga
     1861 catcgagcca gaccgcgagc ggcgcgtggc ccggacgctg gccgtcgagc acgtgcgcga
     1921 ggaagcgcgc cggctgcggc gcgccgtgcg cattgccggg ccagtcgacc agcggccggc
     1981 cttcggcgtc gatgccgctc cagagcccct cggtgccgcc gctgcccgcc ggaaccggcg
     2041 cgggtgctgc cggaatccgc agcgtgatct gcttcatgct cagttctccg cgatcttgct
     2101 gcccttgatg accacgtcgc cgctgccctt gatcgtgatc ttcgcgccct tgatcaggat
     2161 ggtgccgtcc ttcttcagcg tcagctcggc cgagccggtc ttcagcgcga tctcgtcatc
     2221 ggcgaccagc tggatgcgct tggcctgcag cgagtagtcc ttcttgacgg tttccttgca
     2281 gttctcgccg acctcggtgg tcaggttctt gccgatcttg ttggtctggt ccttgtcgat
     2341 ggtctcggtg acggcgccgc cgacctcgat cgagcggttg ccgccgatcg tcaccgaatg
     2401 cgccccgccg accacgtcgg tcttctgcag gccgacctcc tcgctcatcg ccgcgccgac
     2461 ggcgatgttc agcaggccgc cgacggtcag ctccatcgcc gcgccgacgg tttcggaata
     2521 attgatgccg acggtctcgg tcaggttcga ggcgaccgag acctccatgt tcatgtcgat
     2581 gcgctcgaca tggttgccga cgatgtgcac ggtcttgtgc cggtcgacgg tctcggactt
     2641 gtcgcgcccg accaccagcg tgcggtcgcg cccggtggtc tcggagcggt cgtggccgac
     2701 cgacagggtc tggtcgttct cgacctcgtg cagctcgtcc ttctcggcgt ggatgaacag
     2761 ctgctcggag cccttgagat cctccatgcg gatctcgttg cagttcgccg tcgagccgcc
     2821 cttgctggag cggctgcgcc agccgctctg cgtggcgttg gccggcagcg cgaacggcac
     2881 cggctgctcg ccgttgtaga cgcggccggt gatcagcggc cggtccggat cgccttcgag
     2941 gaagtcgacg acgacctcct ggccgatgcg cggaatcatc atcccgcccc agcccttgcc
     3001 ggcccagacc tgcgcgacgc ggatccagca cgagctgtgc tcgtcctggc cgtcgtagcg
     3061 gtcccagtgg aagtgcacct tcacgcgccc gtacttgtcc gtgaagattt cctcgccggc
     3121 cggaccgacg acgatcgcgg tctgcggccc gagcacctgt gccggcggcg tcagcggctc
     3181 gctgcgaaac ggcagctggt tgtcctgcgc ggcgatgtcg agttcgaagt cgagtttccc
     3241 ggcgttcggg ccgctgtggt aggcgtcctg cgtcagccgg tattcggccg acgtgatcag
     3301 cagttcgatg ttgagcttgt cgttcgggca gccgctcagc gtgaacagcg caccgcacga
     3361 caggccgcgg gcatcgccgg tgaagcggca ttcggcggcg cgcgtgcgca gggcttcgag
     3421 ctgcgtgcgc gcgtagtgct cgccggggct ggtctgcgtg taggcgccgg ggtagtcgta
     3481 gcgcttgtag ccggcggccg gaccgctcgc gctcgaggcg gccagcagct gcgccgacgg
     3541 gcgctcgaag tcgaagtcgt tcagcgtcag cgccgaggtc tgcacgctca gcgcctgcga
     3601 ccacgcccac aggtgatcgc gcggccggcg cccgagctgg tcgggcggga agtacggcac
     3661 ttcctcgtag ccgggctgcg cggcgaggct ggcgttgtca tcgacgagca ccagcacatg
     3721 gcgcccgtcc tcgtggcgga agtaatagaa gatgccttcc ttctccagca ggcggctgac
     3781 gaagtcgaaa tcggtctcgc ggtactgcac gcagtattcg cgcggcgcgt agctgccgtt
     3841 gagcttcagt tcgtagtcgg caaagccgag gttctcgaac acctgcttga cgatgtccgg
     3901 cacgctcttc gcctggaaga tgcggcagtc cgagcagtgc gtcagcagcc agaaccacgg
     3961 ccgcagccgc acgcggtagg tggcgatgcg cccgtcgctg ccgctctgcc cgaagctcgc
     4021 gacgatgccg tggaaccagc gcgagccgtc gccggacggc agttcgagct cgacgccgag
     4081 cggcttgccg agcagcccgg ccgggtcgat cgcgaagtcc tcggaggcga gttcgagttc
     4141 gtattcgaac aggcgcccga gccgctcgcg gccgtgcagc cggcgcagca gcagcttgtc
     4201 ggtaccgagg ctgcagctga ccgtgaccgg tcggttcgcc tgcgtgatcg gcatcggggg
     4261 tctccgggga tgcgttcagt ccgcgcaagc atagccgggc gtcgccggcc ggcgcgcgag
     4321 cacgaccaga ccctcgaccg gcgcgccgcg ctcgcgacgc agcacgaccg gctggctgcg
     4381 cagctgcccg aagccggccc cggcgagcac gcgcagcgga taatccgggg cgtggctgta
     4441 gcggccgcag gcattgagcc ggtgatcaag ggcttcggtg tgggtttcga gcgtgaacgc
     4501 cagccagccg cccgggcgca gcgcggcagc ggcagcggcg gcaaagtccg cgagcggccc
     4561 gaagtaacag agtgtgtcgg cggcgaccag caggtcgaac gcgccgggat gagcattgag
     4621 ccaggcacac agttcggcgg tttcgagtgc gtcgtacagc gccagcgacg ccgcctgttc
     4681 gagcatcttc ggtgacaggt cgacgccgct cagctgccgc gcatacgggc gcagcagcgg
     4741 gccgcacagg ccggtgccgc agccggcatc gagcacgtcg agcgcggcgg ccggggcgtc
     4801 gagcaccagc gccagcgcat cggcgaggcg ctcgggcgcg tggtaatcga gcgtcccgcg
     4861 caggtgcgcg tcgaaatggc cggcgtactg gtcgaacagc ccgcgcacga cggcatcgct
     4921 gcagcgcgcc ggcgccggtg cgccgccgca ggcggcaagg cgcagttcgg cgagcggatg
     4981 gccgggttcg agcgcgagcc aggcgcggta ggccgcggcg gcctcgtcga gccggccgag
     5041 gcgcgacagc gcatggccga gcgcgcgcgt ggcaccgagg tgctgcggat caagcgcgag
     5101 cgccgcgcgg tgatgcacgg ccgcggcctc gtattcgtga cagcgttcaa gcagcgtcgc
     5161 cagccggtgg tgatcggccg ccgagctggc ttcgagttcg agcgcgtgcc ggtaggcctc
     5221 gatcgcctcg tgccgccggc cggcggcatg cagcacgacg ccgaggttgt tccacgcggc
     5281 gaccagcgcc ggctcggcgg cgagggcggc gcggtattcg cgctcggcct cgtcgagccg
     5341 gccaagggtc tgcagcacgt tgccatggtt gctgcgcagg cccgcgtggc cgggggcgac
     5401 cgcgacggcg cgggcgagcc attcaagcgc cgcgtcctcg tgcccgcgct ggaattcgag
     5461 cagaccgagg aagtgcagcg catcgggctg atccggcagt acggcgagga tctgccggta
     5521 gaccggctcc gccgcatcga gccggccctc gcgctgccag ccgaccgcga gcgcgagcgc
     5581 ctgttcgagc gtgacggctt cggtcggggg gtcttcaccg ctgtccgggt acatggcggg
     5641 ttctcctgca ggcgcttgcg tgccggcgag cgtagcagcc gcgaccggcg cgccgggccc
     5701 gggggcaagc acggcttatg cacggtcaac ccgcgcccat accgccatcg gcttgccgct
     5761 gtccggggca ggcgaccata gttggcgccc gcccgccgac cccgccgcac cgacatgcat
     5821 ccgcccgccc tgcgcatccg ccagctcgtc aagacctacc gcagcggcgt caccgcgctg
     5881 cacggcatcg acctcgacgt gccggacggc gacttcttcg cgctgctcgg cccgaacggc
     5941 gccggcaagt cgacgacgat cggcatcgtc agctcgctgg tcaacaagac cgccggccgc
     6001 gtcgagatct tcggccacga cctcgaagcc caggcctggg cggcgaagcg gctgatcgga
     6061 ctggtgccgc aggaattcaa cttcaaccag ttcgagccgg tcatcgagat cgtcgtcaac
     6121 caggccggct attacggcat cccgcgccac gtcgcccgcc cgcgcgccga ggaactgctc
     6181 gaacggctcg ggctcgcggc caaggcccgc gacatggcgc gcgagctctc cggcggcatg
     6241 aagcggcggc tgatgatcgc gcgcgcgctg atccacgccc cgcgcctgct gatcctcgat
     6301 gaaccgaccg ccggcgtcga catcgagctg cgccgctcga tgtgggagtt cctgcgcgag
     6361 accaacgccg ccggcacgac catcatcctg accacgcact acctcgaaga ggccgagagc
     6421 ctctgtcgca acatcgcgat catcgaccgc ggccggctgg tcgagcacac cgacatgaag
     6481 tcgctgctcg cgcgcctgca caccgaaacc ttcgtgctga acctcgccgc gccgctcgtc
     6541 gcgctgccgc tgctgcccgg ctacgtgctc gatcgcgtcg acgacacgac gctggaggtc
     6601 gaggtcagca aggagaccgg catgaacgcg ctgtttgccg gcctggccgc acacggcatc
     6661 gaggtgctga gcctgcgcaa caagtcgaac cggctcgaag agctgttcat ccgtctcgtc
     6721 gagagcggcg ccgaggcccg cgccgccgga acccccgcat gaaaccgacc ccgcccgaca
     6781 tcctcgcccc ggcgctcggc gccggcgaag cgccgccgtc cgagttcgcc ggctggtgga
     6841 ccgcgttcgt gacgctgctg atcaaggagg tcctgcgctt cgcgcgcatc tggatccaga
     6901 ccatcctgcc gccggccgtg accacggcgc tgtacttcgt gatcttcggc aagctgctcg
     6961 gcgcgcagct gccgccgatg gacggcatcg gctacccgca gtacatcgcg cccggcctga
     7021 tcatgctggc gatcatcacc aacagctatg cgaatgtggt ttcgagcttc tacggctcga
     7081 agttccagca caacatcgag gaactgctgg tcgcgccgat gccgaactgg ctgatcctcg
     7141 ccggcttcgt cggcggcggc gtactgcgcg ggctggcggt cggggtggtc gtgacgatga
     7201 tcgcgctgac cttcaccgac ctgcacgtca cgcactgggg cgtgaccgtc ggcgtcgtgt
     7261 tcctgaccgc ggtgctgttc tcgctggccg ggctgatcaa cggcatctac gcgaacagct
     7321 tcgatgacat cgcgatcgtg ccgaccttcg tgctgacgcc gctgacctat ctcggcggca
     7381 tcttctattc gatcaacctg ctgccggact tctggcagaa cgtgtcgctg gtcaacccga
     7441 tcctgtacat ggtcaacgcc ttccgctacg gcatcctcgg cgtgtccgac atcggcatgc
     7501 tgcaggcctt ctcgctgatc ggcgccttcg tgctcgggct cggcgccttc gcgctgtggc
     7561 tgctgaaacg cggggtcggc atccgcaact gaagccccgc ccgccgcctc cggccgggct
     7621 cagtcaccga cgaagggatt ggcattgccc tgcagcgcgc ggatgcggcg gttgcgttcg
     7681 cgctcgaagc tgtccggcgg gtcctgccgg ttccaggcct cgtagagctt gcggtcctga
     7741 tcggagagcc ggaaaccgta ggtctgcgcc atgtacagca tcgtgcgcgc gatgtcgccg
     7801 cggatgcgcg ccggcggctc gacgcgccgc tccccggcgt cgatctcgaa gtcgcagcgg
     7861 ccgtaggcgc gctgctcgcc ggcgagcatg ccccagttgt aatcctggcg atcgccgttg
     7921 acctcgccga ccaccggata caggttgtgc aggtcgttgt gcgcggcgcg gaacaccgga
     7981 tcgaccttct cgcagcactc gcgcccgccg agacgccggc cgccggccgt cgtgcattcg
     8041 gcgaactcgc gcggctcacg ccagcactgg cggaagttgc cgaactgcgc ggccgggaac
     8101 acgtgctcga tctcgatgcg cgtcgcgcgg ctggcattgc tgcgcgcctc cagcccgcag
     8161 ccggcggcat cgacctgctt gtcggcatcg aagcggcagc cgcagtagaa atccacgcgc
     8221 cggtcgcggt agacctcgcc gagcagctgg cgtttggcgg tctcgaagct cttcggcgtc
     8281 gccggcaggt tcgagggcac cggatcggca gccctggcac gggcctgcgc gtcctcgcga
     8341 tcacagccgg cgagcagcag caggccggcg agcagcaggc agagaacacg aagcatgccg
     8401 ggctgaccgg catcggggcg cagggatcgg gcggtcatgg gcggggctgt gcaggacatc
     8461 ggcatccgtg ccgggatcgg atcgggaccg cggcaggggc cgggaaccac agatgcgaaa
     8521 ggcgccgcag gcgcccttcg gggtgaagca gacggggagc gtccggccta cagcgcttcg
     8581 ctgccggtct cgccggtgcg gatgcggatc gcctgttcga ggttgaacac gaacagcttg
     8641 ccgtcgccga tcttgccggt gttggcggcg cgcgcgatcg cgtcgatgac gcgctcgaca
     8701 ttcgactgct cgcaggcgat ctcgaccttc accttgggca ggaagtcgac gacgtactcg
     8761 gcgccgcggt acagctcggt atgacctttc tgccggccga
//
LOCUS       DAOQIE010000159         8796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4045_length_8796_cov_7.893262, whole genome shotgun sequence.
ACCESSION   DAOQIE010000159 DAOQIE010000000
VERSION     DAOQIE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4045_length_8796_cov_7.893262"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK144_10775"
     CDS             complement(<1..539)
                     /locus_tag="PK144_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF66256.1"
                     /translation="MRKATHPIPSASVHPARARLRLSVLAVCVALAAGCAAVGPDYER
                     PPVEVPGQWRLPQPSVNELANGAWWQQFADPALDALIQAALDENRDLRIAASRVEEYR
                     GRYGATRAEQFPQVSASGSGGRARLAESQRLPGQPATGTTWQVDLGVSFELDLWGRLR
                     RATESARADLLAQEEARRTV"
     gene            complement(532..3732)
                     /locus_tag="PK144_10780"
     CDS             complement(532..3732)
                     /locus_tag="PK144_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNF66257.1"
                     /translation="MISRFFIDRPVFAGVLSIVIVIAGLAAMSGLPIAQFPEITPPLL
                     QITASYPGADAETASGTVAAPIEQQVNGVDNMIYMQSNNSNSGDTGLNVYFDIGTDVD
                     RVLTDLTNRANLANPQLPQDVTRNGVVIKKSSPNILMAITILSDGSRDPNEVANYTSI
                     NIVDELKRIPGANQVSMFGLTDYAMRIWLRPDRMAELGITTADVRKAIQEQNTQPAAG
                     RIGQEPTAGPVQLSFPVTARGRLSTPEEFDDIMVRSNPDGSSIRIRDIARSELGIRSY
                     DVSSQLNGKDATVILVYQQPGANALTVATDIRAALDRLSKNFPSGVSYVVPYDTTKFV
                     EVSIEAVIHTFFEAVVLVVVVVFLFLQNWRATVIPLLAVPVSIIGTFAGMYALDFSIN
                     TLTLFGMILAIGIVVDDAIVVVESVEHNMAQGMSPKEAARRTMDEVSGPVVAIVLVLC
                     AVFVPVGFLGGITGQLYKQFAITIAISVVISGIVALTLSPALAAVLLKPSHGEKNIFF
                     RGFEAGFHKITGGYLLGATFLIKRALLGTVLFAGVLAIAYGLFRTVPTSFVPDEDQGY
                     LIVAAFLPDGASLERTREVAEQIAKEAQQNPAVENVLALGGFNMLDSQNKPNAATVFV
                     PLKDWKERKDPAQYADAVRKQLQQKFAASIGDAMVLVFNPPPIPGLGTTGGFEFWVQN
                     RGEGGTAALEKAVHELIAKSKEEPALAGLSTLIRANSQQLFVDLDRDRARSLGVQVTD
                     VFDLLQGLFGTVYVNDFNKFGRTYRVILQAEPDYRTRPEDINKVYVRASSGQMVPLSA
                     LVQLRYTTGPDNVARFNGFVAARINGGPAPGYSSGQALAAMEKLAREHLPPNMYFEWA
                     GQAFQEKKAGSSSALAFGFGLVMVFLILAAQYEKWTLPLSVLVAVPFGVCGALIAVFA
                     RGLNNDVYFQIGLVTLIGLAAKNAILIVEFAVEYRHAGRSAMDAALEAARLRFRPIVM
                     TSLAFILGCVPLAISSGAGANSRISIGTGVIGGMLGATVLAIFFVPLFYRLFDRGESQ
                     GHGHAEAPSGVVPDVHALPPDPSAPLPPAPEGDRHA"
     gene            complement(3749..4888)
                     /locus_tag="PK144_10785"
     CDS             complement(3749..4888)
                     /locus_tag="PK144_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF66258.1"
                     /translation="MTAPRPASLAGLAGVCLFAALAAAPAARAQQAPPRPPAEVTVVE
                     VAPRNTPAQFEATGKTESSRLVEIRARVEGYLDRIAYQEGSVVKQGDLLFQLDPKPFE
                     AALDSARAALAQAQARAVNASATLRRVRPLAQEKALSQKDLDDAVAADLEAQAAVAAA
                     KAQVRTAELNLGYTTIRSPLTGKAGDSAQREGSLVTPGSAGLLTTLVQTDPMWVVFGL
                     GEQDVLRFRSGVAAGTLKTPGQGKVAVEIVLADGRVFPQQGVITYVAPTVDAQTGTVT
                     LRAEVPNPDGSLSPGQFVRTRAVNIELVDALMVPQRAVMQGAKGKFVYVVVDGKAEVR
                     PVEPGQFYGDQWIITKGLKAGDPVVVDGAIKLAPGAPVKVTGPAK"
     gene            5224..7857
                     /locus_tag="PK144_10790"
     CDS             5224..7857
                     /locus_tag="PK144_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66259.1"
                     /translation="MHACTRRTPARNAAGWLCAAALACSLSALAAPTPPATAQTPSAA
                     SPAQPPADEGWPRVWRHGEQELVIHEPQIDAWDDWARLRLRAAIAVRENGKGEPRYGV
                     LEISTPTEIDRDSNTVLMSKPVYTPRFAQAEPLEAGRLAAIVREVLPDGQPLVVSLDR
                     LLPYLEQQKLEQRAVQVSLDPPLIFHSTRPATLLGFYGAPEFRPVGDSGLQAAVNTPA
                     TVLLDAAADGPAYYLLLGESWLTTRSLRDGPWTQPAAMPAGFAKLPEESWGELRQAGT
                     GKHLAETPAVYYAEAPAELLITRGAPKYRPVRGTKLQAISNTESAAFLHLGERRHYLL
                     IAGRWFRAASLEGPWEAATTSLPADFAKIPETDPLAWVLASVPGTEAAEDAVRLASVP
                     QKATLQRADLKFSANYAGEPRFRPIEGSDGVRYATNASDPVLAVADRYYAVKDGVWFE
                     AAAPAGPWQVCTSVPPALYTIPASSPMHNVTYVQVYDSTPTTVTTGYTAGYNGQYVMN
                     GLLLFGAGMALGYALHDHDDDYWYRPWYWGGAPRPYYSYGYGAVYRPGYGYGAAGWAR
                     GPYAAAAGRTWYNPATGAWARGGAVATPYGVAGGRAGYNPWTDTYGARIGGANAYGSW
                     GRSYVERGDDWVAAGHRSGPAGNTGWVRTSEGGKALVRNGQVYAGKDGEVYRRTGRGE
                     WQQYSGGQRWQAASTARTNAAVSGQPRTGTAQGLRDTAPAGRERPATRPETRPATRPA
                     ERPETRPATRPAERPETRPATRPAERPETRPAPRPETRPAGRPETRPETARPRPETRP
                     QPAPARSDFATRQTPSRAARETAPAGLDRDFGARSRGERSIERSQHSTRSFERSQRAS
                     SGFSGGGGGRVGGGGGGGRRR"
     gene            complement(7951..8502)
                     /locus_tag="PK144_10795"
     CDS             complement(7951..8502)
                     /locus_tag="PK144_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66260.1"
                     /translation="MSMPGTDDPSASSTSKLSPAERDKARADIRALRERTLADLYRRR
                     PATRAEIEKAAGYAVIGASGLNVVLLVGAQGAGVVVDRRSGQETFVRMLRAGTGPGLG
                     YMDYRFVLVFKSATALDQFLKLGADVSAQGNVTVKPSGSGESTDTMTSFTPGVSVYQL
                     MDKGVNVQANWGGTKYYRDPDLN"
BASE COUNT         1334 a         3125 c         3123 g         1214 t
ORIGIN      
        1 acggtgcgcc gcgcctcctc ctgcgcgagc agatcggcgc gcgccgactc ggtggcgcgg
       61 cgcagccggc cccacaggtc gagctcgaag gacacgccga gatcgacctg ccaggtcgtg
      121 ccggtcgccg gctggccggg caggcgctgg ctctcggcga gccgcgcccg gccgcccgag
      181 ccgctcgccg agacctgcgg gaactgctcg gcacgggtcg caccgtagcg tccgcggtac
      241 tcctcgacgc gcgacgcggc gatgcgcagg tcgcggttct cgtcgagcgc ggcctggatc
      301 agcgcatcga gcgccggatc ggcgaactgc tgccaccagg cgccgttggc cagctcgttg
      361 acgctcggct gcggcagccg ccactggccc ggcacctcga ccggcggacg ttcgtagtcg
      421 ggaccgaccg ccgcgcagcc ggccgcgagc gcgacacaga cggcgagcac ggacaggcgc
      481 aggcgcgcgc gcgcaggatg cacggaagca gacgggatcg ggtgcgtggc cttacgcatg
      541 gcggtcgccc tccggggcgg gcggcagcgg tgcggacgga tcgggaggca gcgcgtgcac
      601 gtcgggcacg accccggacg gcgcttcggc gtggccgtgg ccctggcttt caccccggtc
      661 gaacagccgg tagaacagcg gcacgaagaa gatcgcgagc acggtcgcgc cgagcatgcc
      721 gccgatgacg ccggtgccga tcgagatgcg gctgttggcg ccggcaccgc tgctgatcgc
      781 gagcggcacg cagccgagga tgaacgcgag cgaggtcatc acgatcggcc ggaagcgcag
      841 ccgtgcggcc tcgagcgcgg catccatcgc cgagcgcccg gcgtggcggt actcgacggc
      901 gaactcgacg atcaggatgg cgttcttcgc cgccagaccg atcagcgtca ccaggccgat
      961 ctggaagtac acgtcgttgt tgaggccgcg cgcgaagacc gcgatcagcg cgccgcagac
     1021 gccgaagggc accgccacca gcacgctgag cggcagcgtc catttctcgt actgcgccgc
     1081 gaggatcagg aacaccatga ccagaccgaa gccgaaggcg agcgccgacg acgagccggc
     1141 cttcttctcc tggaaggcct ggccggccca ctcgaagtac atgttcggcg gcaggtgctc
     1201 gcgcgcgagc ttctccatcg ccgccagcgc ctgaccggac gaatagcccg gtgccggccc
     1261 gccgttgatg cgcgcggcga cgaagccgtt gaagcgcgcg acgttgtccg ggccggtcgt
     1321 gtagcgcagc tgcaccagcg ccgacaacgg caccatctgg ccgctcgacg cgcgcacgta
     1381 gaccttgttg atgtcctcgg gccgcgtgcg gtagtccggc tcggcctgca ggatcacgcg
     1441 gtaggtacgg ccgaacttgt tgaagtcgtt gacgtagacg gtaccgaaca gcccctgcag
     1501 caggtcgaac acgtcggtga cctgcacgcc gagcgagcgc gcgcggtcgc ggtcgagatc
     1561 gacgaacagc tgctgcgagt tggcgcggat cagcgtgctc aggccggcga gcgccggttc
     1621 ctccttggat ttcgcgatca gctcatgcac ggccttctcg agcgccgccg tgccgccctc
     1681 gccgcggttc tgcacccaga actcgaaacc gccggtggtg ccaaggccgg gaatcggcgg
     1741 cggattgaac accagcacca tcgcatcgcc gatgctggcg gcgaacttct gctgcagctg
     1801 cttgcgcacc gcatcggcgt actgcgccgg gtccttgcgt tccttccagt ccttcagcgg
     1861 cacgaacacg gtcgcggcgt tcggcttgtt ctgcgagtcg agcatgttga agccgccgag
     1921 cgccagcacg ttctcgaccg ccgggttctg ctgcgcctcc ttcgcgatct gctcggccac
     1981 ctcgcgcgtg cgttcgaggc tggcaccatc gggcaggaag gcggcgacga tcagatagcc
     2041 ctgatcctca tcgggcacga agctggtcgg caccgtgcgg aacagcccgt acgcgatcgc
     2101 gagcacgccg gcgaacagca ccgtgccgag cagcgcgcgc ttgatcagga aggtcgcgcc
     2161 gagcaggtag ccgccggtga tcttgtggaa gcccgcctcg aagccgcgga agaagatgtt
     2221 cttctcgccg tgcgagggct tcagcagcac cgcggccagg gccggcgaca gcgtcagcgc
     2281 cacgatgccg gagatcacga ccgagatcgc gatcgtgatc gcgaactgct tgtagagctg
     2341 gccagtgatg ccgccgagga agccgaccgg cacgaacacc gcgcagagca ccagcacgat
     2401 ggcgaccacc gggccgctga cctcgtccat ggtccggcgc gcggcctcct tcggcgacat
     2461 gccctgcgcc atgttgtgct cgaccgactc gacgacgacg atcgcgtcgt cgacgacgat
     2521 gccgatcgcg aggatcatgc cgaacagcgt cagcgtgttg atcgagaagt cgagcgcata
     2581 catgccggcg aaggtgccga tgatcgacac cggcaccgcc agcagcggga tgaccgtcgc
     2641 gcgccagttc tgcaggaaca ggaacacgac gacgacgacg agcacgaccg cctcgaagaa
     2701 ggtgtggatg accgcctcga tcgacacctc gacgaacttg gtcgtgtcat agggcacgac
     2761 gtagctgacg ccgctcggga agttcttcga cagccggtca agcgcggcgc ggatgtcggt
     2821 ggcgacggtc agcgcgttgg cgcccggctg ctggtagacg aggatcacgg tcgcgtcctt
     2881 gccgttcaac tggctcgaca cgtcgtagct gcggatgccg agttcgctgc gggcgatgtc
     2941 gcggatgcgg atcgaggagc cgtccgggtt cgagcgcacc atgatgtcgt cgaactcctc
     3001 gggcgtggag aggcgcccgc gcgccgtgac cgggaacgac agctgcaccg gtccggcagt
     3061 cggctcctgc ccgatgcgcc cggccgccgg ctgcgtgttc tgctcctgga tggccttgcg
     3121 cacgtcggcg gtggtgatgc cgagctcggc catgcggtcg ggccgcagcc agatgcgcat
     3181 cgcgtagtcg gtgaggccga acatcgacac ctggttggcg ccggggatgc gcttgagctc
     3241 gtcgacgatg ttgatcgatg tgtagttggc gacctcgttc gggtcgcgcg agccgtcgga
     3301 caggatcgtg atcgccatca ggatgttcgg cgaggacttc ttgatcacga cgccgttgcg
     3361 cgtcacgtcc tgcggcagct gcggattggc gaggttggcg cggttggtga ggtcggtcag
     3421 cacgcgatcg acatcggtgc cgatgtcgaa gtacacgttc aggccggtgt caccgctgtt
     3481 cgagttgttc gactgcatgt agatcatgtt gtcgacgccg ttgacctgct gctcgatcgg
     3541 cgcggcgacc gtgccggagg cggtttcggc gtcggcgccg gggtaggacg cggtgatctg
     3601 cagcagcggc ggcgtgatct ccgggaactg cgcgatcggc aggccgctca tcgccgcgag
     3661 gccggcgatg acgatgacga tcgacagcac gccggcgaac accgggcggt cgatgaagaa
     3721 gcgcgaaatc atctcggggc gcctccgctc acttggccgg gccggtgact ttcaccggcg
     3781 cgcccggtgc gagcttgatc gcaccgtcga cgacgaccgg atcgccggcc ttgaggccct
     3841 tggtgatgat ccactggtca ccgtagaact gccccggctc gaccgggcgc acctcggcct
     3901 tgccgtcgac gaccacgtac acgaacttgc ccttcgcgcc ctgcatcacg gcgcgctgcg
     3961 gcaccatcag cgcatcgacg agttcgatgt tgaccgcgcg cgtgcgcacg aactgccccg
     4021 ggctcagcga gccgtcgggg ttcggcacct cggcgcgcag cgtcacggtg ccggtctgcg
     4081 catcgacggt cggcgcgacg taggtgatca cgccctgctg cgggaacacg cgcccgtcgg
     4141 cgagcacgat ctcgacggca accttgccct gccccggcgt cttcagcgtg ccggcggcca
     4201 cgccgctgcg aaagcgcagc acgtcctgct cgccgaggcc gaacacgacc cacatcggat
     4261 cggtctgcac cagcgtcgtc agcagcccgg ccgagcccgg cgtcaccagc gagccctcgc
     4321 gctgcgccga atcgccggcc ttgccggtca gcggcgagcg gatcgtcgtg tagccgaggt
     4381 tcagctccgc cgtgcgcacc tgcgccttgg cggcggcgac cgcggcctgg gcttcgagat
     4441 cggcggccac cgcatcgtcg aggtccttct ggctcagcgc cttctcctgc gcgagcgggc
     4501 gcacgcgcct gagcgtcgcg gaggcattga cggcgcgtgc ctgcgcctgc gcgagcgcgg
     4561 cgcgggcgct gtcgagtgca gcctcgaacg gcttcggatc gagctggaac agcaggtcgc
     4621 cctgcttcac gaccgagcct tcctgatagg cgatgcggtc gagatagccc tcgacgcgcg
     4681 cgcggatctc gacgaggcgc gagctttcgg tcttgccggt ggcctcgaac tgcgccggcg
     4741 tgttgcgtgg cgcgacctcg acgacggtca cctcggccgg cggacggggc ggagcctgct
     4801 gggcacgggc ggcgggcgcg gcggcaagcg cggcgaacag acagacgccg gcgaggccgg
     4861 ccagcgatgc ggggcgcggg gcggtcacgg tcttgggctc cctgggatcg gatggatggc
     4921 ggtagaagag cttgcagcat cccgcagcgc ggcctgcccc gcacggtccc gcgcgcgcgg
     4981 aatcatgtat cggcaggccg gggtgacggg ggtgtgcgtc aggatggatc gatgcacccg
     5041 gctggcacgg aagcggtgtg cgggcaccgg ttccgggcgt accgggcttc cctggacggc
     5101 aagcatagaa aaaccggctg gacattactg cgccggatgc tgcactgcat gtagcaatgc
     5161 taaccggcac cggcccgtcc cgtcccgttg ccggtccccg cccctgtccg gagtatttcc
     5221 gtcatgcacg cctgtacccg ccgtacaccg gcccgcaacg cggccggctg gctctgtgcc
     5281 gccgcgctcg cctgctcgct gtccgccctc gccgccccga ccccgcccgc caccgcccag
     5341 accccgtctg ccgccagccc cgcacagccg ccggccgacg agggctggcc gcgcgtctgg
     5401 cgccacggcg agcaggaact cgtgatccac gagccccaga tcgacgcctg ggacgactgg
     5461 gcccggctgc gcctgcgcgc ggcgatcgcg gtgcgcgaga acggcaaggg cgagccgcgc
     5521 tacggcgtgc tcgaaatctc gacgccgacc gagatcgacc gcgacagcaa caccgtgctg
     5581 atgagcaagc ccgtgtacac gccgcgcttc gcgcaggcgg agccgctcga agccgggcgg
     5641 ctcgccgcga tcgtgcgcga ggtgctgccc gacggccagc cgctggtcgt gtcgctcgat
     5701 cgcctgctgc cctacctcga acagcagaag ctcgaacagc gcgcggtgca ggtgagcctc
     5761 gatccgccgc tgatcttcca cagcacccgg ccggcgacgc tgctcggctt ctacggtgcc
     5821 cccgaattcc ggccggtcgg cgacagcggc ctgcaggcgg cggtcaacac gccggcgacc
     5881 gtgctgctcg atgccgctgc tgacggcccg gcctactacc tgctgctcgg cgaatcgtgg
     5941 ctgacgacgc gcagcctgcg cgacgggccg tggacgcagc cggcggcgat gccggcgggc
     6001 ttcgcaaagc tgcccgagga gagctggggc gaactgcgcc aggccggcac cggcaagcac
     6061 ctcgccgaga ccccggccgt ctattacgcc gaagcaccgg ccgagctgct gatcacgcgc
     6121 ggggcgccga agtaccgccc ggtgcgcggc acgaagctgc aggcgatcag caacaccgag
     6181 tccgccgcct tcctgcacct cggcgagcgt cgccattacc tgctgatcgc cgggcgctgg
     6241 ttccgcgccg ccagcctcga aggcccgtgg gaagcagcga cgacctcgct gccggccgac
     6301 ttcgcgaaga tccccgaaac cgatccgctc gcctgggtgc tcgcgtcggt gcccggcacc
     6361 gaggcggccg aggatgccgt gcggctcgcc tcggtgccgc agaaggcgac gctgcagcgc
     6421 gcggacctga aattcagcgc gaactacgcc ggcgagccgc gcttcaggcc gatcgagggc
     6481 agtgacggcg tgcgctatgc cacgaacgcc tccgatccgg tgctcgccgt cgccgatcgc
     6541 tactacgcgg tcaaggacgg cgtctggttc gaggccgccg cgcccgcggg cccctggcag
     6601 gtctgcacga gcgtgccgcc ggcgctctac acgatcccgg cgagcagccc gatgcacaac
     6661 gtcacctacg tgcaggtcta cgacagcacg ccgaccacgg tcacgaccgg ctacacggcc
     6721 ggctacaacg gccagtacgt gatgaacggc ctgctgctgt tcggggccgg catggcgctc
     6781 ggctacgcgc tgcacgatca cgacgacgac tactggtacc gcccgtggta ctggggcggc
     6841 gcgccgcgcc cgtactactc ctacggctac ggcgcggtct atcgccccgg ctacgggtac
     6901 ggcgccgccg gctgggcgcg cggcccgtat gcggccgccg ccgggcgcac ctggtacaac
     6961 ccggcgaccg gcgcctgggc gcgcggcggc gcggtcgcga ccccgtacgg cgtcgccggc
     7021 ggccgggccg gctacaaccc gtggaccgac acgtatggtg cgcgcatcgg cggagccaac
     7081 gcctacggca gctggggccg cagctacgtc gagcgcggcg acgactgggt cgccgccggc
     7141 caccgcagcg gcccggccgg caacaccggc tgggtgcgga cctcggaagg cggcaaggcg
     7201 ctggtgcgca acggacaggt ctacgccggc aaggacggcg aggtctaccg gcgcaccggt
     7261 cgcggcgaat ggcagcagta ctcgggcggc cagcgctggc aggcggcgag cacggcgcgc
     7321 acgaacgccg ccgtcagtgg ccagccgcgc accggcaccg cgcagggcct gcgcgacacc
     7381 gctcccgccg gacgcgaacg ccccgccaca cgcccggaaa cccgtcccgc cacccgtccg
     7441 gctgaacgcc cggaaacccg ccccgccacc cggccggccg aacgcccgga aacccggccg
     7501 gccacccgtc cggctgaacg cccggaaacc cgtcccgccc cgcgtccgga gacccggccg
     7561 gccgggcgac cggaaacccg tcccgaaacc gcccgcccgc gccccgaaac ccggccgcag
     7621 cccgcaccgg cacgcagcga cttcgcgacc cgccagacgc cttcccgtgc cgcccgcgag
     7681 accgctcccg ccggcctcga ccgcgacttc ggcgcccgct cgcgcggtga gcgcagcatc
     7741 gaacgcagtc agcacagcac ccgcagcttc gagcgcagtc agcgcgcgag cagcggcttc
     7801 agtggcgggg ggggcggacg tgtcggcggg ggcggcggcg gcggacgccg gcgctgaggg
     7861 tgcctcccgc agcgcccgca agggcagaac ggccccggcc tcgcgacgcc cgcgggccgg
     7921 gcgccgcacc gggccggggg cgctgcctgc tcagttcagg tccggatcgc ggtagtactt
     7981 ggtgccgccc cagttggcct gcacgttcac gcccttgtcc atgagctggt agacgctgac
     8041 gccgggcgtg aacgaggtca tcgtgtcggt ggactcgccg ctgccgctgg gcttcaccgt
     8101 cacgttgccc tgggcgctca cgtcggcgcc gagcttcagg aactgatcga gcgccgtggc
     8161 gctcttgaag accagcacga agcggtagtc catgtagccg aggcccggac cggtgccggc
     8221 gcgcagcatg cgcacgaagg tttcctgacc gctgcgccgg tcgacgacga cgccggcgcc
     8281 ctgggcaccg accagcagca cgacgttgag gccggaggca ccgatcacgg catagccggc
     8341 ggccttctcg atctcggcgc gcgtcgccgg acgccggcgg tacagatcgg ccagcgtgcg
     8401 ctcgcgcagc gcacggatgt cggcccgtgc cttgtcgcgc tcggcgggcg agagcttgct
     8461 cgtggacgat gccgacggat catcggtgcc cggcatggac atgcaggcag ccaggctcgt
     8521 gcccagcagg acggcggccg tgaaacggca caggggactg gatttcaacg tgaatacccc
     8581 cagggtcggc tcaggaaaac gtcaagagga tcaaggctgc ggttcagccc agtgcgtgat
     8641 agccggcatc gacgtagccg atgttgccgg tgatgttgct gccggtctcg gcgatgtaga
     8701 aggctgcgag ccggccgaca tcctcgggcg tcgccagccg cccgagcggc gagcgcgtgg
     8761 tcgcatcggc catcagctcg tcgaagtcct tgatgc
//
LOCUS       DAOQIE010000160         8750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4067_length_8750_cov_8.196665, whole genome shotgun sequence.
ACCESSION   DAOQIE010000160 DAOQIE010000000
VERSION     DAOQIE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4067_length_8750_cov_8.196665"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK144_10800"
     CDS             <1..192
                     /locus_tag="PK144_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66261.1"
                     /translation="LSVGHDALGLGHDALSVGRDASSVGHDALGLGHDALNVGRDASS
                     VGHDALSVGRKASDLARKA"
     gene            518..952
                     /locus_tag="PK144_10805"
     CDS             518..952
                     /locus_tag="PK144_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF66262.1"
                     /translation="MHETFTKSMARNIYYGGAAFFVLVFLGLTFDTTHTLPKRDNRAN
                     LTETVALGKKVWENNNCIGCHTLLGEGAYFAPELGNVYKRFGNSTEAIKAFIRSRPEN
                     GVPGRRSMPQFHLTDQELEGIAQFLKYSSEINTANWPPNIQG"
     gene            984..2390
                     /locus_tag="PK144_10810"
     CDS             984..2390
                     /locus_tag="PK144_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF66263.1"
                     /translation="MQYLSQKVAKPYFVAAIGLFAGQILFGLIMGIQYLVGDFLFPAI
                     PFNVARMVHTNLLIVWLLFGFMGASYYLVPEEAERELESPLLAKILFWVFLIAGALTV
                     VGYLAVPYASLAKLTGNDVLPTMGREFLEQPTITKVGIVVVALGFLYNVGLTILKGRK
                     TVINLVLLTGLAGLAVLFLFSFYNPDNLVLDKFYWWWVVHLWVEGVWELILGSILAFV
                     LIKVTGVDREIIEKWLYVIIAMALITGIIGTGHHYFWIGTPEYWQWLGSVFSAMEPLP
                     FFAMTVFAFNMVNRRRREHPNQAATLWALGTSVMAFLGAGVWGFLHTLAPVNYYTHGT
                     QITAAHGHMAFYGAYVMIVLTIISYAMPMLRGQTAANSRRSQVLEMWSFWLMTVSMVF
                     ITLFLTGAGILQVYLQRYSAQPLPFMVVQEQISLFYWLRLIAGLVFTIGLGAYIASFF
                     VGRTEPEAVAMKSVPVTA"
     gene            2526..3308
                     /locus_tag="PK144_10815"
     CDS             2526..3308
                     /locus_tag="PK144_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNF66264.1"
                     /translation="MSELPYYAPAGNEIALFEAAWRERLPVLLKGPTGCGKTRFVAHM
                     AARLGRPLYTVACHDDLTAADLVGRHLIGDGGTWWSDGPLTRAVREGAICYLDEVVEA
                     RKDTTVVLHPLTDDRRILPIERTGETLAAPAEFMLVVSYNPGYQNLLKGLKPSTRQRF
                     VAMAFDYPDAAREREILMREAGIDAGLAERLVRLGHGLRALKDHDLDEGASTRLLIYA
                     ATLMRAGLAPRAACRAALIEPLTDDTATLLALQEVVDVAFPG"
     gene            3289..3687
                     /locus_tag="PK144_10820"
     CDS             3289..3687
                     /locus_tag="PK144_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66265.1"
                     /translation="MSRFPAERRPDADGRRLAVRAQALYLANLLILPGLAFAALLWLR
                     RRRAGVPLLAQAHLDAALHASLWALALLPGVAGLLVLVAGLRAPATWMTLILWVVSMH
                     TALVLWGLLALAAALAGRLPPPPWRGRISG"
     gene            3986..7294
                     /locus_tag="PK144_10825"
     CDS             3986..7294
                     /locus_tag="PK144_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66266.1"
                     /translation="MNAAPLTVPPAPLTAALLLAAAYVLAGAAGLALALPPDTEIAVW
                     PAAGVAAGGLLAGGLRLWPAVWIGSFVINLWLRLSLLGQPPTASAFLIPAGIACGAAA
                     QAWVGARLSAALLARPHPLAHGTETLSFLLRVGPLACLISATTATLLLVASASVPVEA
                     AGGLFWTWWCGDTLGVLLFTPIVLAWMAPAHRDWHARRRLLSLPLVASLLLTTTAVHQ
                     ASEAERRRTEQDFERQALPLATAIIGTLQRHVEAVQSLADFLAAVPPEPQAFRRYATG
                     QIARLPGLRVLQWSPRVPAAALAEFEAAQRDAGRPGYRVFSIDDASGRRVPLPALPAY
                     YPIQFNEPATGNEAALGLDVHARRISRPALEQSRRSGSVAVTPGLRLVQETGEQTGVV
                     FYQAVPPGRPGSEPNGFAGGVLRLGDTLAAAIAPLPRSALIVRLTDETQGAPLPLQVD
                     PDWPAHLSAGQHWSQPLVVGDRQWRLEIAPRQALPTGQHGPVAHAVQAGALLFNGVLA
                     AFLLVTSGRTTLIENIIATRTQRLQREIEDRRRAEAGLRQAAIVYEYTREGILVLDAN
                     GRVQAVNPAFERISGFTLAQWQGRRPQKLFVPRQRRRLLADIRRTLASGGHWQGETWN
                     LDHEGSPYPSWLTVSAVPGENGQPGGYIAVFSDISTIKQSQAELEHLAHHDPLTGLPN
                     RLLLGARLSHAILRARREGERMALMFLDLDRFKIVNDSLGHRIGDRLLVAAAARLAEG
                     LRASDTLARLGGDEFVLLVDRIADPEVVGRLATRLVEACNAPFTIDEHELYLGASVGI
                     ALYPDDGLDAETLLRNADAAMYEAKAHGRNTYRYYLPSLTDSAEARVRIEGELRRALA
                     HGELRLHYQPQVELADARVIGCEALVRWQHPTRGLVPPNDFLPLAEDTGLILPIGAWV
                     LDEACRQARAWLDAGHELRVAVNIAVPQVMRQDLPALVSEILARHRLPARYLELELTE
                     SLLLRDPERGAHVMSQLAELGVGLAIDDFGTGYSSLAYLKRLPIDRLKIDRLFVRELP
                     EDSDDCAIVRATLDMASHLRLSVVAEGVETAAQAEFLLAHGCGHAQGWRYGKAVPPAE
                     FPLVPVLVVPPDTP"
     gene            7461..>8750
                     /locus_tag="PK144_10830"
     CDS             7461..>8750
                     /locus_tag="PK144_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNF66267.1"
                     /translation="MNQPKHRSTAPLLAVTSIALGLGLAPGSAHAEDASHPGVGQGEV
                     RYQGAPSGIDAAQAKDQNAGAPQMTAEEFAQAKKIFFERCAGCHGVLRKGATGKPLTP
                     DVTRARGTDYLKVFINYGSPAGMPNWGSSGELTEQQVDMMARYIQIEPPLPPEFGMKE
                     MKDTWKVIVPPGQRPTKKMNAFNIQNIFSVTLRDSGEVALVDGDTKQIINIVKTGYAV
                     HISRLSASGRYLYVIGRDARINLIDLWMEKPDNVAEIKIGLEARSVDTSKFKGYEDRF
                     AIAGSYWPPQFVIMNGDTLEPKKIVSTRGMTVDTQDYHPEPRVAAIVASHEHPEFIVN
                     VKETGKILLVNYADIDNLQVTEIGAARFLHDGGWDATHRYFLTAANKSNKVAVVDSRE
                     RKLAALVDVGAIPHPGRGANFVDPQFGPVWATSHLGDD"
BASE COUNT         1263 a         3184 c         2936 g         1367 t
ORIGIN      
        1 ttgagcgtcg gtcacgacgc tttgggcctc ggtcacgacg ctttgagcgt cggtcgcgac
       61 gcttcgagcg tcggtcacga cgctttgggc ctcggtcacg acgctttgaa cgtcggtcgc
      121 gacgcttcga gcgtcggtca cgacgctttg agcgtcgggc gcaaggcttc ggaccttgcg
      181 cgcaaggctt gaaacctcgc atccgcggct tccagcgtcg cggatgggct tgcgagcctt
      241 gcgcccgtcg ctggcgcagc agcgaccgcc ctgcgccgcg cctcacccgc tccggccggg
      301 caggcggatg tgccgccgga caccgggcca tgcgcgatga ccccggccgg aaatcgtttc
      361 gacacacccc cggagttaga ccggcttacc cacgaggtgg agtggccggc agcgcttgat
      421 gcgagtcaag gtgcgcagcc ccttgccaat cgctcaatgc cgccagcgcc ggagcctgca
      481 cggtttccgg acttcgccac caacctgggg aaatgccatg cacgagactt tcaccaagtc
      541 catggctcgg aacatctatt acggaggcgc agccttcttc gtactggtgt tcctcggcct
      601 gaccttcgat accacgcata cgctgccgaa acgcgacaac cgcgccaacc tgaccgagac
      661 cgtcgcgctc ggcaagaagg tctgggaaaa caacaactgc atcggctgcc acacgctgct
      721 cggcgagggt gcgtatttcg cgcccgaact cggcaacgtc tacaagcgct tcggcaacag
      781 caccgaggcg atcaaggcct tcatccggag ccggccggaa aacggcgtgc cgggccggcg
      841 ctcgatgccg cagttccacc tgacggatca ggaactcgaa ggcatcgcgc aattcctgaa
      901 gtacagctcc gaaatcaaca ccgccaactg gccgccgaac atccagggct gatccgcctg
      961 cgcccgcccg aggacatcac gtcatgcaat acctgtcgca aaaggtcgcg aaaccgtatt
     1021 tcgtcgccgc gatcgggctg ttcgccggcc agatcctgtt cggcctgatc atgggcatcc
     1081 agtatctggt cggagacttc ctgttcccgg ccattccgtt caacgtggcc cgcatggtcc
     1141 acaccaatct gctgatcgtc tggctgctgt tcggcttcat gggcgcgagc tattacctcg
     1201 tgcccgagga ggccgagcgc gagctcgaaa gcccgctgct cgcgaagatc ctgttctggg
     1261 tgttcctgat cgccggcgcg ctgaccgtcg tcggttatct ggccgtgccg tatgcgtcgc
     1321 tcgcgaagct gaccggcaat gacgtgctgc cgaccatggg ccgcgaattc ctcgaacagc
     1381 cgaccatcac caaggtcggc atcgtcgtgg tcgcgctcgg tttcctctac aacgtcggcc
     1441 tgaccatcct caagggccgc aagaccgtca tcaacctggt gctgctgacc ggactcgccg
     1501 ggctggccgt gctgttcctg ttctcgttct acaaccccga taacctggtg ctcgacaagt
     1561 tctactggtg gtgggtcgtg cacctgtggg tcgagggcgt ctgggagctg atcctcggtt
     1621 cgatcctcgc cttcgtgctg atcaaggtca ccggcgtcga tcgcgagatc atcgagaagt
     1681 ggctgtacgt gatcatcgcg atggcgctga tcaccggcat catcggcacc ggccaccact
     1741 acttctggat cggcacgccg gagtactggc agtggctcgg ctcggtgttc tcggcgatgg
     1801 aaccgctgcc gttcttcgcg atgacggtgt tcgcgttcaa catggtcaac cgccgccgcc
     1861 gcgagcaccc gaaccaggcc gcgacgctgt gggcgctcgg cacctcggtg atggccttcc
     1921 tcggcgccgg cgtctggggc ttcctgcaca cgctcgcgcc ggtcaactac tacacgcacg
     1981 gcacgcagat caccgccgcg cacggccaca tggccttcta cggcgcctac gtgatgatcg
     2041 tgctgaccat catcagctac gcgatgccga tgctgcgcgg ccagaccgcc gccaacagcc
     2101 gccgctcgca ggtgctggag atgtggtcgt tctggctgat gacggtgtcg atggtgttca
     2161 tcacgctgtt cctgaccggc gccggcatcc tgcaggtcta cctgcagcgc tacagcgccc
     2221 agccgctgcc gttcatggtc gtgcaggagc agatcagcct gttctactgg ctgcggctga
     2281 tcgccggcct ggtgttcacc atcggcctcg gtgcctacat cgcgagcttc ttcgtcgggc
     2341 gcaccgagcc ggaagcggtg gcgatgaagt cggtaccggt gacggcctga agccccggcc
     2401 ggccccgggc gtgagggacg accgtccctc acgccttttt catccgggtc ccgggtgccg
     2461 cgcggatggt cctttggctg cgcggtgctc ccctgccctt tcccctgccc cgtcccggag
     2521 ccgccatgtc cgagcttccg tattacgccc cggccggcaa cgagatcgcg ctgttcgagg
     2581 ccgcctggcg cgagcggctg ccggtgctgc tcaagggccc gaccggctgc ggcaagacgc
     2641 gcttcgtcgc gcacatggcc gcccgcctcg gccggccgct gtatacggtc gcctgccacg
     2701 acgacctgac cgccgccgat ctggtcggcc ggcacctgat cggcgacggc ggcacctggt
     2761 ggagcgacgg gccgctgacg cgcgcggtgc gcgagggtgc gatctgctac ctcgacgaag
     2821 tcgtcgaggc gcgcaaggac accaccgtcg tgctgcaccc gctgaccgac gaccggcgca
     2881 tcctgccgat cgagcgcacc ggcgaaaccc tcgccgcacc ggcggaattc atgctcgtgg
     2941 tctcatacaa ccccggctac cagaacctgc tgaagggcct gaagccctcg acccgccagc
     3001 gcttcgtggc gatggccttc gactacccgg acgcggcacg cgagcgcgaa atcctgatgc
     3061 gcgaggccgg catcgacgcc gggctggccg agcgcctcgt gcgcctcggc cacggcctgc
     3121 gtgcgctgaa ggatcacgac ctcgacgagg gcgcctcgac gcggctgctg atctacgccg
     3181 cgacgctgat gcgcgccggc ctcgcgccgc gcgccgcctg ccgggcggcg ctcatcgaac
     3241 cgctgaccga tgacacggcc acgctgctcg cgctgcagga agtcgtcgat gtcgcgtttc
     3301 ccggctgagc gcaggcccga tgccgacggc cgccggctgg cggtgcgcgc gcaggcgctg
     3361 tacctcgcca acctgctgat cctgccgggc ctcgcgttcg cggcgctgct gtggctgcgc
     3421 cgccggcgtg ccggtgtgcc gctgctcgcg caagcgcacc tcgatgcggc gctgcacgcg
     3481 agcctctggg cactggccct gctgccgggc gtggccgggc tgctggtgct ggtcgccggt
     3541 ctgcgtgcgc cggcgacctg gatgacgctg atcctgtggg tggtcagcat gcacacggcg
     3601 ctggtgctct gggggctgct ggcgctggcc gcggccctgg caggacggct gccgccgccg
     3661 ccgtggcgcg gccgcatcag cgggtgagcg cgatcacgcg cgaacggcct gtcctgcccg
     3721 tcctgtctct gcctatcctt ctcccgcgcc cggccgggcc ctgccgagcc ggtgcgtgcg
     3781 gccggcacga aacggctgca acaagcgatt tcatccggca ttgaggtttt ccggtaccgt
     3841 cgcgctgccg cgcgcagcgg atcccggcat tcgcgaacgc tgactttgcg gctagccttg
     3901 cgcgcggcat gtccgccccg ccccgaaccc gctcttctgg tccggcatgc cgtttccggc
     3961 cccagtgctg ccgtcggttt cgtcgatgaa cgccgccccc ctcactgtac cgcccgcccc
     4021 cctgacggca gccctgctgc ttgccgcagc ctatgtgctt gccggcgcgg ccgggctggc
     4081 gctggcgctg ccgcccgata ccgagatcgc cgtctggccg gcggccggtg tcgccgccgg
     4141 cggcctgctc gccggcggcc tgcggctgtg gccggcggtg tggatcggct ccttcgtgat
     4201 caacctgtgg ctgcgcctga gcctgctcgg ccagccgccg acggcctcgg cgttcctgat
     4261 tccggccggc atcgcctgcg gggccgccgc gcaggcctgg gtcggcgcgc ggctgagcgc
     4321 ggcgctgctc gcgcgcccgc acccgctcgc gcacggcacc gagacgctgt ccttcctgct
     4381 gcgcgtcgga ccgctcgcct gcctgatcag cgccacgacc gccacgctcc tgctggtcgc
     4441 cagcgcctcg gtgccggtcg aggccgcggg cgggctgttc tggacctggt ggtgcggcga
     4501 tacgctcggc gtgctgctgt tcacgccgat cgtgctggcc tggatggcgc cggcgcaccg
     4561 cgactggcat gcgcgccggc ggctgctgag cctgccgctc gtggcctcgc tgctgctgac
     4621 cacgacggcc gtgcatcagg cgagcgaggc cgaacgccgg cgcaccgagc aggatttcga
     4681 acgccaggcg ctgccgctcg cgaccgcgat catcggcacg ctgcagcgcc acgtcgaggc
     4741 cgtgcagtcg ctcgccgact tcctcgctgc cgtgccgccg gagccacagg ccttccgccg
     4801 ctatgcgacc ggccagatcg cacggctgcc gggcctgcgc gtgctgcagt ggtcgccgcg
     4861 cgtgccggcg gcggcactgg cggaattcga ggccgcccag cgcgacgccg gccgcccggg
     4921 ctaccgggtg ttctcgatcg acgatgccag cggcaggcgc gtgccgctgc cggcgctgcc
     4981 ggcgtattac ccgatccagt tcaacgagcc tgcgaccggc aacgaggcgg cgctcggtct
     5041 cgatgtccac gcccgccgca tctcgcgccc ggcgctggaa cagtcgcgcc gcagcggcag
     5101 cgtcgcggtc acgcccggcc tgcgcctggt gcaggagacc ggcgagcaga ccggcgtcgt
     5161 gttctatcag gcggtgccgc cgggccgccc cggttccgaa ccgaacggct tcgccggcgg
     5221 cgtgctcagg ctcggcgaca cgctggccgc ggcgatcgcg ccgctgccgc gcagcgccct
     5281 gatcgtgcgc ctgaccgacg agacccaggg cgcgccgctg ccgctgcagg tcgaccccga
     5341 ctggccggcg cacctgtccg ccggccagca ctggagccag ccgctggtgg tcggcgatcg
     5401 ccagtggcgg ctggagatcg cgccgcgcca ggcgctgccg accggccagc acggaccggt
     5461 cgcgcacgcg gtgcaggccg gtgcgctgct gttcaacggc gtgctcgcgg ccttcctgct
     5521 cgtgacctcg gggcgcacga cgctgatcga gaacatcatc gccacgcgca cgcagcgcct
     5581 gcagcgcgag atcgaggacc gccgccgcgc cgaggccggg ctgcggcagg cggcgatcgt
     5641 ctacgagtac acgcgcgagg gcatcctggt gctcgatgcc aacggccgcg tgcaggcggt
     5701 gaacccggcc ttcgagcgca tcagcggttt cacgctcgcg cagtggcagg gccggcgccc
     5761 gcagaagctg ttcgtgccgc gccagcgccg gcggctgctc gccgacatcc gccgcacgct
     5821 cgcctccggc ggtcactggc agggcgagac ctggaacctc gatcacgaag gctcgccgta
     5881 tccgtcctgg ctgacggtca gcgccgtacc gggcgagaac ggccagcccg gcggctacat
     5941 cgcggtgttc tcggacatct cgacgatcaa gcagtcgcag gcggaactcg aacacctcgc
     6001 ccaccacgac ccgctgaccg ggctgccgaa ccggctgctg ctcggggcgc gcctgagcca
     6061 cgcgatcctg cgcgcccggc gcgagggcga gcgcatggcg ctgatgttcc tcgatctcga
     6121 ccgcttcaag atcgtcaacg acagcctcgg ccaccgcatc ggcgaccggc tgctggtggc
     6181 ggcggcggca cggctcgccg agggcctgcg cgccagcgac acgctggcgc gcctcggcgg
     6241 cgacgagttc gtgctgctgg tcgatcgcat cgccgatccg gaggtggtcg gccggctggc
     6301 gacgcgcctg gtcgaggcct gcaacgcccc gttcacgatc gacgagcacg agctctacct
     6361 cggggccagc gtcggcatcg cgctgtatcc cgatgacggg ctcgacgccg aaacgctgct
     6421 gcgcaacgcc gacgccgcga tgtacgaggc caaggcccac gggcgcaaca cctaccgcta
     6481 ctacctgccg agcctgaccg acagtgccga ggcgcgcgtg cgcatcgagg gggaactgcg
     6541 ccgcgcgctc gcccacggcg aactgcgcct gcactaccag ccgcaggtcg aactcgccga
     6601 tgcccgcgtg atcggctgcg aggcgctggt gcgctggcag catccgaccc gcgggctggt
     6661 gccgccgaac gacttcctgc cgctcgccga ggacaccggc ctgatcctgc cgatcggcgc
     6721 ctgggtgctc gacgaggcct gccgccaggc gcgcgcctgg ctggacgccg gccacgaact
     6781 gcgcgtggcg gtcaacatcg ccgtgccgca ggtcatgcgt caggacctgc cggcgctggt
     6841 ctcggaaatc ctcgcgcgcc accgcctgcc ggcgcgctat ctggagctgg agctgaccga
     6901 atcgctgctg ctgcgcgatc cggagcgcgg cgcacacgtc atgtcgcagc tcgccgagct
     6961 cggcgtcggg ctggcgatcg atgacttcgg caccggctat tcctcgctcg cctacctcaa
     7021 gcgcctgccg atcgaccggc tgaagatcga ccggctgttc gtgcgcgagc tgcccgagga
     7081 cagcgacgac tgcgcgatcg tgcgcgcgac gctggacatg gccagtcacc tgcgcctgtc
     7141 ggtggtcgcc gaaggcgtcg agaccgccgc gcaggcggaa ttcctgctcg cgcacggctg
     7201 cggtcacgcc cagggctggc gctacggcaa ggccgtgccg cccgcggagt tcccgctggt
     7261 gccggtgctg gtggtacccc cggatacccc ttagggatca gtctcctgtg tgcccgggcg
     7321 cggcttgatg agcgtcaagg gtcggccccg ccccccgggc gtcagatagc cgcggcaagc
     7381 aggcgcaacg gtcgcatccg ctcgaccgca gcgcacgagt tccgcagcca tccgcaagtc
     7441 gcacggggga gcaccagaac atgaatcagc cgaaacaccg cagcacggcc ccgctgctgg
     7501 ccgtcacgtc gatcgccctc ggcctcggcc tcgcgcccgg cagcgcgcac gccgaggacg
     7561 ccagccaccc gggcgtcggc cagggcgagg tccgctatca gggcgcgccg agcggcatcg
     7621 acgccgcgca ggcgaaggac cagaacgccg gcgcgccgca gatgacggcc gaggagttcg
     7681 cgcaggccaa gaagatcttc ttcgagcgct gcgccggctg ccacggcgtg ctgcgcaagg
     7741 gcgcgaccgg caagccgctg acgcccgatg tcacgcgcgc acgcggcacc gattacctga
     7801 aggtgttcat caactacggc tcgccggccg gcatgccgaa ctggggcagc tcgggcgagc
     7861 tgaccgagca gcaggtcgac atgatggcgc gctacatcca gatcgagccg ccgctgccgc
     7921 ccgaattcgg catgaaggaa atgaaggaca cctggaaggt catcgtgccg cccgggcagc
     7981 gcccgacgaa gaagatgaat gccttcaaca tccagaacat cttctcggtg acgctgcgcg
     8041 actccggcga ggtggcgctg gtcgacggcg acaccaagca gatcatcaac atcgtcaaga
     8101 ccggctacgc cgtgcacatc tcgcggctgt cggcctcggg ccgctacctg tacgtgatcg
     8161 gccgcgatgc gcgcatcaac ctgatcgacc tgtggatgga gaagcccgac aacgtcgccg
     8221 agatcaagat cggcctcgaa gcgcgctcgg tcgacacctc gaagttcaag ggctacgagg
     8281 accggttcgc gatcgccggc tcctactggc cgccgcagtt cgtgatcatg aacggcgaca
     8341 cgctggagcc gaagaagatc gtctcgacgc gcggcatgac cgtcgacacg caggactacc
     8401 accccgagcc gcgcgtcgcc gcgatcgtcg cctcgcacga gcacccggag ttcatcgtca
     8461 acgtcaagga gaccggcaag atcctgctgg tcaactacgc cgacatcgac aacctgcagg
     8521 tcaccgagat cggcgccgcg cgcttcctgc acgacggcgg ctgggacgcg acgcaccgct
     8581 acttcctgac cgccgcgaac aagtccaaca aggtcgccgt ggtcgactcc agggagcgca
     8641 agctcgccgc cctcgtcgat gtcggcgcga tcccgcaccc ggggcgcggc gcgaacttcg
     8701 tcgatccgca gttcggtccg gtctgggcca ccagccatct cggcgatgac
//
LOCUS       DAOQIE010000161         8679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4112_length_8679_cov_7.429615, whole genome shotgun sequence.
ACCESSION   DAOQIE010000161 DAOQIE010000000
VERSION     DAOQIE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_8679_cov_7.429615"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PK144_10835"
     CDS             complement(<1..1635)
                     /locus_tag="PK144_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HNF66268.1"
                     /translation="MNAPLAPAIAIVGLACRYADAGTPQALWQMALAQRRAFRRLPRE
                     RLNLADYAEAVAGADGTHASEAAVLHGWHFDRERFRIPAATVRASDPAHWLALEVAAD
                     ALADAGFGDGAGLPRLTTGVVIGNTLTGEVSRAASLRLRWPYARRTVAARLAAEGWPA
                     ARQREFLAGLEACWKAPFPVPDADTLAGGLANTIAGRICNHYDLGGGGWSVDGACASS
                     LLAIAQACSALAAGDLDVAIAGGVDLSLDPFELVGFARVGALAREDMRVFDAAGTGFW
                     PGEGCGLALLMRAADARAQGLRPYALIRGWAVASDGSGGLTRPEVAGQRRAIERAWQR
                     AGRSPADAALFECHGTGTAVGDPVELQALAAARGAVTGMAAAVGSIKANIGHTKAAAG
                     AAGLIKTALALREGLLPPTTGCRTPHPLFAGAASGLRVLADGEPWPDAAARLAGVSGF
                     GFGGIDVHLALSAADEAPAAAATLSETQRRWLRSAQDTELLLLAAPDAAALDARIASL
                     AACAAQLSRAELVDLACTLSAQADARLPARAALLAST"
     gene            complement(1632..2552)
                     /locus_tag="PK144_10840"
     CDS             complement(1632..2552)
                     /locus_tag="PK144_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enediyne biosynthesis protein UnbU"
                     /protein_id="HNF66269.1"
                     /translation="MAGPDLRLAALRRFATAITVLNVAGYTLLGFEPAWAHPLVALAT
                     AYALELLLETLEARSRGLRPRFLGGGFGGFVDFLLPAHITGLAVSMLIYTNARLAPVA
                     FAVAVGIGSKYLLRVPVGPGGATRHFLNPSNTGIAVTLLLFPWIAITPPYQFAENLSG
                     AGDWLLPCVFIGLGSFLNAKLTRKLPLILGWLGGFALQAGLRAWLADTPLAAGLAPMT
                     GIAFLLFTFYMVTDPATTPVAPRAQFAFGAGVAAAYGVLMALHVVFGLFFALFAVCSI
                     RGAWLYAQHLRLSRRVASPAGVLGKPETAP"
     gene            complement(2552..4465)
                     /locus_tag="PK144_10845"
     CDS             complement(2552..4465)
                     /locus_tag="PK144_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019032452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNF66270.1"
                     /translation="MLKSSTALGRLLHSPPRWLSRALALLAILALYGLARLPEPSADE
                     RAALAAAFRFVQGPLEGVPGPMRGVREVSPAFAGISAWISSVGAGVALADFDGDGLAN
                     DACLVDPRTDLVSVLPVPGLVAPGSTARFAAVALDPRPLPYDRTMAPMGCLPGDFDGD
                     GRMDALVYYWGRTPLIFLGDGRGGFRPLELAAQPARWYTNSATLADLDGDGRPDLVIA
                     NYFPDGSRLLDPASRAPETMQHSMSRAFNGGPAHFFLEAGSHAGRPAFTEVSVDLPAD
                     VRGGWTLALGAADLDGDLLPELYFANDFGPDRLLHNRSMPGALRFVSLEGRRGLFDPA
                     SKVLGRDSFKGMGIDFADLDGSGRLGMFVSNIAAEHALQESHFAFLPGGPAGLMQQGI
                     APYTDQSEALGLSRSDWGWDLKLADFANDGRLELVQATGFVRGSVNRWPELQELAMAN
                     DELLPDPRIWADFRPGADLSGNAHPPFFVRGADGRYVDLAADIGLGRPMISRGLAIAD
                     VDGDGDLDLAIANQWEPSLFLRNDCPRCGRSLELVLRVPLADGASRAAIGAEARVRLP
                     DGRVRVAQVDGGNGHSGRRAPELHFGLGASEAPVPVTLRWRAADGQVHVEERRLAPGR
                     HVLLLDAAAPGGA"
     gene            complement(4459..5415)
                     /locus_tag="PK144_10850"
     CDS             complement(4459..5415)
                     /locus_tag="PK144_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HNF66271.1"
                     /translation="MARSAGPACALLGIGREAASFERRGFLPAPPAVQQRLEGIGRTF
                     IDGYRAALSAASAEALAAQLDAEIPLAGRGFAYEGAGMGLALLDLLLPWTASRLQAFL
                     AGPALAHCYIVHVGAGWALARLPWGEGYRRRLDPLLGWLAYDGWGFHRGYFGGGRIPA
                     AYPARLGMAARRVHDQGLGRSLWFVCGADPWRVAMAVAGLDAGRHADLWAGVGLAATY
                     AGGAGDAALAELRRLAGAHAGWLAQGAAFAAKARLHAGNPTADTERGCRALCGCDAVT
                     AAAATDRALQTLPADGPEPAYALWRHRIHALLRPRSEEPVPC"
     gene            complement(5507..6283)
                     /locus_tag="PK144_10855"
     CDS             complement(5507..6283)
                     /locus_tag="PK144_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF66272.1"
                     /translation="MNRLSIWLCRLAGLPLLWLTLAVPAHAVPLANGLDGFRTTPGSS
                     FTLPAALPAGFFGSKNGTDSELIAAGTVIAVEGNIALPFATAKKFLVGPGCHTGSTNH
                     HCYEMPVIELFDTVVRRTTDAELTPGGGPVAIGVMLEYLSLRSSSPLEVHYGSEPSSF
                     FDIFVELDGPQTAGSISIHDFQSDNTGLMDTVLPVTYSVTFAEVGGGLQTTINGLQDV
                     MQSQNNSIFVVPEPGTLWLLGAAAVAGLRRRRRIPDRTRA"
     gene            complement(6356..7183)
                     /locus_tag="PK144_10860"
     CDS             complement(6356..7183)
                     /locus_tag="PK144_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF66273.1"
                     /translation="MQTRLGPPALALTGAFWLAAPAMAVPLVIDPGFDLFATDAAGTF
                     ATLPGIGPVQLEGLPFDTGAGDRGNVDTILLRRGPALTLNGIGDSGSMPLELVALSLR
                     SVAPVQISPGVFADVHVNVNKPIEDIGAPGGNDDGACDPGESCTAANPERPLDALPAS
                     AGALTITRTGENGGTFDSFFDVFAEITLTLPGTDTVIGSSTFQDTFVQQSGLWSSTPG
                     VGDAHTAQLPAGGFLPGVVAELALLASHRAGPAIVPEPATLALFGLGALWLSRRRPG"
     regulatory      7622..7698
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            7772..>8679
                     /locus_tag="PK144_10865"
     CDS             7772..>8679
                     /locus_tag="PK144_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNF66274.1"
                     /translation="MNAVVQTEQSSFSDFHVADLSLAGWGRKEIAIAETEMPGLMAVR
                     AEYATSQPLKGARITGSLHMTIQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAA
                     AGIPVFAYKGESLDDYWDYSHRIFEWADGGYSNMILDDGGDATLLLMLGTRAESDRSV
                     LAKPTCEEEIALFASIERTLARDPKWYSTRLACIKGVTEETTTGVHRLYQLQKEGRLP
                     FPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVLGYGDVGKGCAQSL
                     RGLGATVWVTEIDPICALQAAMEGYRV"
BASE COUNT         1355 a         3084 c         3171 g         1069 t
ORIGIN      
        1 ggtcgaagcg agcagcgccg cgcgggccgg cagccgcgca tcggcctgcg ccgacagcgt
       61 gcaggcgagg tcgaccagct cggcgcgcga cagctgcgcc gcgcaggccg ccagcgaggc
      121 gatccgcgca tcgagggccg cggcgtcggg tgcggcgagc agcagcagtt cggtgtcctg
      181 ggcgctgcgc agccagcggc gctgggtctc gctcagcgtc gcggccgccg ccggcgcctc
      241 gtcggccgcg gacagcgcca gatgcacgtc gatgccgccg aagccgaaac cgctgacgcc
      301 ggcgagccgt gcggcagcgt cgggccaggg ctcgccgtcg gcgagcacgc gcagtccgct
      361 cgccgcgccg gcgaacagcg gatgcggcgt gcgacagccg gtggtcggcg gcagcagtcc
      421 ttcgcgcagc gccagcgcgg tcttgatcag cccggccgcg ccggccgcgg ccttggtgtg
      481 gccgatgttg gccttgatcg agccgaccgc ggccgccatg ccggtcacgg cgccgcgcgc
      541 cgcggccagc gcctgcagct cgaccggatc gccgaccgcg gtgccggtgc cgtggcattc
      601 gaacagcgcc gcgtcggccg gggagcggcc ggcgcgctgc caggcgcgct cgatcgcacg
      661 gcgctggccg gccacctcgg gccgcgtcag cccgccgctg ccgtccgagg ccacggccca
      721 gccgcggatc agcgcgtacg gccgcaggcc ctgcgcccgc gcatcggctg cgcgcatcag
      781 cagcgccagt ccgcagccct cgccgggcca gaagccggtg ccggcggcat cgaacacgcg
      841 catgtcctcg cgcgccagcg ccccgacgcg cgcgaagccg accagctcga acggatcgag
      901 gctgagatcc acaccgccgg cgatggcgac atcgagatca ccggcggcga gcgcgctgca
      961 ggcctgcgcg atcgcgagca gcgaggacgc gcaggcgcca tcgacgctcc agccgccgcc
     1021 gccgaggtcg tagtggttgc agatgcgccc ggcgatcgtg ttcgcgaggc cgccggccag
     1081 cgtgtcggca tcgggcaccg ggaacggcgc cttccagcac gcttcgagtc cggcgaggaa
     1141 ttcccgctgc cgcgccgccg gccagccttc ggccgcgagc cgggcggcga ccgtgcgccg
     1201 cgcgtacggc cagcgcaggc gcaggctggc ggcgcgcgag acctcgccgg tcagcgtgtt
     1261 gccgatcacg acgccggtcg tcaggcgcgg cagcccggcg ccgtcgccga agccggcatc
     1321 ggcgagcgca tcggcggcga cttcgagtgc cagccagtgc gccgggtcgc tcgcgcgcac
     1381 ggtcgcggcc ggaatgcgga agcgctcgcg gtcgaagtgc cagccgtgca gcacggcggc
     1441 ctccgaggca tgcgtgccgt cggccccggc cacggcttcg gcgtagtcgg cgaggttcag
     1501 gcgctcgcgc ggcagccgcc ggaaggcccg gcgctgcgcg agcgccatct gccagagcgc
     1561 ctgcggcgtg ccggcgtccg catagcggca ggcgagaccg acgatggcga tcgccggcgc
     1621 gagcggcgcg ttcatggtgc ggtctccggc ttgccgagca cgccggccgg cgacgcaacc
     1681 cgccggctca ggcgcaggtg ctgcgcgtac agccaggcgc cacggatcga gcagaccgcg
     1741 aacagcgcga agaacagccc gaacaccacg tgcagcgcca tcagcacgcc gtaggcggcc
     1801 gcgacgccgg cgccgaaggc gaactgcgcg cgcggtgcga ccggcgtcgt cgccgggtcg
     1861 gtgaccatgt agaacgtgaa cagcaggaag gcgatgccgg tcatcggcgc gagcccggcc
     1921 gcgagcggcg tatcggccag ccacgcgcgc aggcccgcct gcagcgcgaa accgccgagc
     1981 cagccgagga tcagcggcag cttcctcgtg agcttcgcgt tcaggaagct gccgaggccg
     2041 atgaacacgc agggcagcag ccagtcgccg gccccggaga ggttctcggc gaactggtac
     2101 ggcggcgtga tcgcgatcca cgggaacagc agcagcgtca cggcgatgcc ggtgttcgac
     2161 gggttcagga agtggcgcgt cgcgccgccc ggcccgaccg gcacgcgcag cagatacttc
     2221 gagccgatgc cgaccgcgac cgcgaaggcc accggcgcga gccgggcgtt ggtgtagatc
     2281 agcatcgaca cggccagccc ggtgatgtgc gccggcagca ggaaatcgac gaagccgccg
     2341 aagccgccgc cgaggaagcg cgggcgcagg ccgcggctgc gcgcctccag ggtttcgagc
     2401 aggagttcga gcgcgtatgc ggtcgcgagc gccaccagcg gatgcgccca ggccggctcg
     2461 aaaccgagca gcgtgtagcc ggcgacattc agcaccgtga tcgcggtcgc gaagcggcgc
     2521 agtgccgcga gtcgcaggtc gggtccggcc atcacgcacc tcccggggcc gcggcatcga
     2581 gcagcagcac gtggcggccc ggtgccagcc tgcgttcctc gacgtggacc tgaccgtcgg
     2641 cggcgcgcca gcgcagcgtc accggcaccg gagcctccga agccccgagc ccgaagtgca
     2701 gctccggcgc gcgccggccc gagtggccgt tgccgccatc gacctgcgcg acacgcacgc
     2761 ggccatcggg caggcgcacg cgcgcctcgg cgccgatcgc cgcacggctc gcgccatcgg
     2821 cgagcggcac gcgcagcaca agttccagcg agcggccgca gcgcgggcag tcgttgcgca
     2881 ggaacagcga cggctcccac tggttggcga tcgcgagatc gaggtcgccg tcgccatcga
     2941 catcggcgat cgcgaggccg cggctgatca tcggccggcc gagcccgatg tcggcggcga
     3001 gatcgacgta acggccatcg gccccgcgca cgaagaacgg cgggtgcgca ttgccgctca
     3061 ggtcggcgcc gggccggaag tcggcccaga tgcgcggatc gggcagcagt tcgtcgttgg
     3121 ccatcgccag ttcctgcagc tccggccagc ggttgacgct gccgcgcacg aagccggtcg
     3181 cctgcaccag ttcgagccgg ccgtcgttgg cgaagtcggc gagcttcagg tcccagcccc
     3241 agtcgctgcg cgacaggccg agcgcctcgc tctgatccgt gtacggcgcg atgccctgct
     3301 gcatcagccc ggccgggccg cccggcagga acgcgaagtg gctctcctgc agcgcgtgct
     3361 cggcggcgat gttgctgacg aacatgccga gccggccgct gccgtcgagg tcggcaaagt
     3421 cgatgcccat gcccttgaac gaatcgcgcc cgagcacctt cgaggccggg tcgaacaggc
     3481 cgcgccggcc ttcgagcgac acgaagcgca gcgccccggg catcgagcgg ttgtgcagca
     3541 ggcggtcggg accgaagtcg ttggcgaagt acagttccgg cagcaggtcg ccatcgagat
     3601 cggccgcgcc gagcgcgagc gtccagccgc cgcgcacgtc ggccggcaga tcgacgctga
     3661 cctcggtgaa ggccggccgg cccgcgtgac tgccggcctc caggaagaaa tgcgccggcc
     3721 cgccgttgaa ggcgcgcgac atcgagtgct gcatcgtttc gggcgcgcgg ctggccggat
     3781 cgagcaggcg cgagccgtcc gggaagtagt tggcgatgac gagatccggg cggccgtcgc
     3841 cgtcgaggtc ggccagcgtc gcgctgttcg tgtaccagcg cgccggctgt gcggcgagtt
     3901 cgagcggccg gaaaccgccg cggccgtcgc cgaggaagat cagcggcgtg cgcccccagt
     3961 agtagacgag cgcatccatg cgcccgtcgc cgtcgaagtc gccgggcagg cagcccatcg
     4021 gcgccatggt ccggtcgtac ggcagcgggc gcggatcgag cgcgacggcc gcaaagcgtg
     4081 cagtactgcc tggtgccacc agccccggca ccggcagcac cgagacgaga tcggtgcgcg
     4141 gatcgaccag acaggcatcg ttggcgaggc cgtcgccgtc gaaatcggcg agcgcgacgc
     4201 cggcgccgac cgaggagatc cacgcggaaa tgccggcgaa cgccggactg acttcgcgca
     4261 cgccgcgcat cggcccgggc acgccttcga gcgggccctg cacgaagcgg aaggcggcgg
     4321 cgagcgcggc gcgctcgtcg gcggacggct ccggcagccg ggccagcccg tacagcgcca
     4381 ggatcgccag cagggcgagc gcccggctga gccagcgcgg cggcgaatgc agcaggcgcc
     4441 cgagggccgt ggaagatttc agcatgggac gggctcctcg ctgcgcgggc gcagcagcgc
     4501 gtggatgcgg tggcgccaga gcgcataggc cggctcgggg ccatcggccg gcagcgtctg
     4561 cagcgcgcgg tcggtggcgg ccgccgcggt gacggcatcg cagccgcaca gcgcgcgaca
     4621 gccgcgttcg gtgtccgctg tcgggttgcc ggcgtgcagc cgggccttgg ccgcgaacgc
     4681 cgcgccctgc gcgagccagc cggcgtgcgc accggccagc cggcgcaact cggccaaggc
     4741 tgcgtcgccg gcgccgccgg cataggtcgc ggccagcccg acgccggccc acagatcggc
     4801 atgccggccg gcgtccagcc cggcaacggc catggccacg cgccaggggt cagccccgca
     4861 gacgaaccac aggctgcggc cgagcccctg gtcgtggacc cggcgggctg ccatcccgag
     4921 ccgcgccgga tacgccgccg gaatgcgtcc gccgccgaag tagccgcggt gaaagcccca
     4981 gccgtcgtag gccagccagc cgagcagcgg atcgagccgc cgccggtagc cctcgcccca
     5041 gggcagccgc gccagcgccc agccggcgcc gacgtggacg atgtagcaat gcgcgagcgc
     5101 cggcccggcg aggaaggcct gcagccgcga cgccgtccac ggcagcagca gatcgagcag
     5161 cgccagcccc atgccggcgc cctcgtaggc aaagccgcgc ccggccagcg ggatttccgc
     5221 atcgagttgt gcggcgagcg cttcggccga ggcagcggac agggcggccc ggtatccgtc
     5281 gatgaaggtc cggccgatgc cttcgagccg ctgctgcacg gccggaggcg ccggcaggaa
     5341 accgcgccgc tcgaagctcg ctgcttcgcg gccgatgccg agcagcgcgc aggcaggtcc
     5401 ggcggatcgg gccatggcgg cctccggaaa agggaaaggc ttcggggaag ccggtgcgcc
     5461 ggcccgcatc gggacgcggc gcagggaggg gatcacacgg cgccgttcag gcccgggtgc
     5521 ggtcgggtat ccggcggcga cggcgcaggc ccgcgacggc cgcggcgccg agcagccaga
     5581 gcgttcccgg ctccggcacg acgaagatgc tgttgttctg cgactgcatc acgtcctgca
     5641 ggccgttgat cgtggtctgc aggccaccgc ccacctcggc gaaggtcacg ctgtaggtca
     5701 ccggcagcac ggtatccatc aggccggtgt tgtcgctctg gaagtcgtgg atgctgatgc
     5761 tgccggcggt ctgcgggccg tcgagttcga cgaagatgtc gaagaagctc gacggttcgg
     5821 acccgtagtg gacttcgagc gggctgctcg atctcagcga caggtattcg agcatcacgc
     5881 cgatggcgac cggcccgccg ccgggggtca gttcggcatc ggtggtgcgg cgcacgaccg
     5941 tgtcgaacag ctcgatgacc ggcatctcgt agcagtggtg gttcgtcgag ccggtatggc
     6001 agccggggcc gaccaggaac ttcttcgcgg ttgcgaacgg cagcgcgatg ttgccttcga
     6061 cggcgatcac agtaccggca gcgatcagct ccgaatcggt gccgttcttg ctgccgaaaa
     6121 agccggccgg cagcgccgcc ggcagcgtga acgaggaccc cggggtggtc ctgaacccgt
     6181 ccagcccgtt cgcgagcggg acggcgtggg cgggaaccgc cagggtcagc cacagcagcg
     6241 gcagaccggc cagtcgacac agccagatcg acaacctgtt cattgcgctt ctcccgatgt
     6301 cggcagcgca gggcgcgctg gatcaagccc ccggagaggg caccgcagcg gtgcttcagc
     6361 ccggtcggcg ccggctcagc cagagggcac cgagtccgaa cagcgccagg gtcgccggct
     6421 ccgggacgat ggccggaccc gcccggtgac tggcgagcag ggccagttcg gcgaccacac
     6481 ccggcaggaa acccccggcg gggagctggg ccgtatgggc atcgccgacg cccggcgtgc
     6541 tgctccagag gccggactgc tgcacgaagg tgtcctggaa ggtgctgctg ccgatcacgg
     6601 tgtccgtgcc gggcagcgtc agcgtgatct cggcgaacac gtcgaagaac gaatcgaagg
     6661 tgccgccgtt ctcgccggtg cgcgtgatcg tcagcgcacc ggccgaggcc ggcagcgcat
     6721 cgagcgggcg ctcggggttc gcggccgtgc aggactcgcc gggatcgcag gcaccgtcgt
     6781 cgttgccacc gggagcgccg atgtcctcga tcggcttgtt gacgttcaca tggacatcgg
     6841 cgaacacgcc ggggctgatc tgcaccggcg caacgctgcg cagcgacagc gcgacgagtt
     6901 cgagcggcat gctgcccgag tcgccgatgc cgttgagcgt cagcgccggc ccccggcgca
     6961 gcaggatggt gtcgacgttg ccgcggtcac cggccccggt atcgaagggc agaccttcga
     7021 gctgcaccgg cccgatgccg ggcagggtcg cgaacgtgcc ggcggcatcg gttgcgaaca
     7081 ggtcgaaccc ggggtcgatc accagcggca ccgccatcgc cggagcggcc agccagaacg
     7141 cgcccgtcag ggccagcgcg ggcggcccca gccttgtctg cattgacatc ttcttctccc
     7201 ccggcctgcc cgatcaaact gacacggaca gatcgacgta tcacctctgt ggtaggccgg
     7261 actgcggccg tgaccggcag cccggaatgc cccggttgtt tcccggtggt cagtcctgac
     7321 aatgcattgg ctacgccaca gcgataaaaa tcaatcagat caactgcatc gaacaaacgc
     7381 actgcgacac tggtttcatg gatgtaaaaa aaccggacat cacgcgtctg tatggctgtg
     7441 tacggcagcc acaggcagca cagtcagggg ccggacggcc cgccgcccat ggacccggcg
     7501 acggttgcgc tgcccgaagc ggcaaaaccc tgtaaagaaa ttgaaataca cgcgtccgga
     7561 cgcgggcctg cacccacgtg ccggcttggc gacaacggcc gtgctccgta tgattcgcgc
     7621 cctgcccgag gagcgctgca acggggaaac acccacccgc caggctcggt caccgtttcc
     7681 caacggcgct cacctgaccc cgtgccgggc acgggatgtc ccgggtgagc cgacgacatc
     7741 tcccccggcc acatcgacag gagccctctc catgaacgct gtggtccaga ccgaacagtc
     7801 ctccttctcc gatttccacg tcgccgacct gtcgctcgcc ggctggggac gcaaggaaat
     7861 cgcgatcgcc gaaaccgaga tgcccggcct gatggccgtg cgcgccgagt acgcgacgag
     7921 ccagccgctc aagggcgcgc gcatcaccgg ctcgctgcac atgacgatcc agaccgccgt
     7981 gctgatcgag acgctggtcg cgctcggtgc cgaggtgcgc tgggcctcgt gcaacatctt
     8041 ctcgacccag gaccatgccg ccgccgcgat cgccgcggcc ggcattccgg tgttcgcgta
     8101 caagggcgaa tcgctggacg attactggga ctactcgcac cgcatcttcg agtgggccga
     8161 cggcggctac tcgaacatga tcctcgacga cggcggtgac gcgacgctgc tgctgatgct
     8221 cggcacgcgc gccgagtccg accgctcggt gctggcgaag ccgacctgcg aggaggagat
     8281 cgcgctgttc gcgtcgatcg agcgcacgct ggcgcgtgat ccgaagtggt attcgacgcg
     8341 gctcgcctgc atcaagggcg tcaccgagga aaccaccacc ggcgtgcacc gcctgtacca
     8401 gctgcagaag gaaggccggc tgccgttccc ggcgatcaac gtcaacgatt cggtcaccaa
     8461 gtccaagttc gacaacctct acggctgccg cgaatcgctg gtcgacggca tcaagcgtgc
     8521 gaccgacgtg atgatcgccg gcaagatcgc ggtcgtgctc ggctacggcg atgtcggcaa
     8581 gggctgcgcg cagtcgctgc gcggcctcgg cgcgaccgtc tgggtcaccg agatcgaccc
     8641 gatctgcgcg ctgcaggcgg cgatggaagg ctatcgcgt
//
LOCUS       DAOQIE010000162         8548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4203_length_8548_cov_8.239256, whole genome shotgun sequence.
ACCESSION   DAOQIE010000162 DAOQIE010000000
VERSION     DAOQIE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_8548_cov_8.239256"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /gene="dnaX"
                     /locus_tag="PK144_10870"
     CDS             complement(<1..1083)
                     /gene="dnaX"
                     /locus_tag="PK144_10870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNF66275.1"
                     /translation="MSHQVLARKWRPKRFAELVGQEHVVRALGNALANGRLHHAFLFT
                     GTRGVGKTTIARILAKSLNCETGITAEPCGVCAACREIDEGRFVDLIEVDAASRTKVE
                     DTRELLENVQYAPARGRYKVYLIDEVHMLSTSSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFSLKRLPAALIQSRLADILDAEGIAHEPAALAALARAGDGSMRDAL
                     SLLDQAIAFGAGRVGALDTAAMLGTIDRQHVVHLAEALAGGDAASVLAAIERLDREAP
                     DYDGVLAELLSLLQRVALAQAVPGASDELYDSDTLVRLAAAFTAEDVQLCYQIALHAR
                     RDLPLAPEPRGGFEMALLRMLCFRPAT"
     gene            complement(1161..1257)
                     /gene="ffs"
                     /locus_tag="PK144_10875"
     ncRNA           complement(1161..1257)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK144_10875"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1464..2738
                     /locus_tag="PK144_10880"
     CDS             1464..2738
                     /locus_tag="PK144_10880"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNF66276.1"
                     /translation="MKLETLAIHAGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVAGNIYTRIMNPTTAVLEARMAALEGGIGALGLASGQAAITYAIQTIAEAGDNIVA
                     TGTLYGGTYNLFAHTLPQYGIEVRFVDYRDPASAAALIDGRTKAVFCETIGNPLGNVV
                     DFEAFSQVAHAHGVPLIVDNTVATPYLCRPFEHGADIIVHSLTKYLGGHGTCIGGVLV
                     DSGKFPWAEHKERFRRLNEPDVSYHGVTYTEALGAAAYIGRARVVPLRNMGAAISPFN
                     SFLVLQGIETLALRMDRHCANALAVAGFLRDHAAVEWVGYPGLPDSPFRPLVDRYMGG
                     KGSGLVTFGVKGGREAGARFQDALQLVTRLVNIGDAKSLACHPASTTHRQLNAAELAK
                     AGVSEDMVRLSIGIEHIDDILADLDQALAATR"
     gene            2792..3385
                     /locus_tag="PK144_10885"
     CDS             2792..3385
                     /locus_tag="PK144_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNF66277.1"
                     /translation="MLHRHLVAWLRSFFVSDFGRPARGPAPLFAALAFVLVVIGVLVL
                     QPWGMSSSAGPAPVGATEDCTVRSVQDGDTVTLACNAQERRVRLWGIDAPELGQGDWG
                     RLARSGLTARLRKENVRLEVLDTDQYGRTVGRIWLGKTDIGLEQVHAGRAAVYREFNN
                     SSEYTTAEAEARRAGRGIWEAPGAQQTPWLWRRLNPR"
     gene            3550..4626
                     /locus_tag="PK144_10890"
     CDS             3550..4626
                     /locus_tag="PK144_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66278.1"
                     /translation="MLPTCTADPSGGVRLDGSWTLAGFACGLPLLPEKPVPRIDAGGL
                     TALDTAGALQILALEAGSDTHWDGLTAGHATLLDLVRAQAARERPPPPPVPGCLEALG
                     RRATAQLCEVRDFLGFFGEMSLAFWRALLLPHRFRWTPFLANIQSAGLAAMPIVGLLS
                     FLLGVVIAYQGGSQLGRYGANIFIVELVSLTMLREMSVIMTAIIVAGRTGSAWTAQIG
                     TMRVTEEIDALRTIGIRPMDMLVIPKVFALMVALPLLTVYADAMGVLGGMVMADLQLG
                     VSFQEFLQRFPQAVSVRSFLIGVGKAPVFALLIALVGCYQGFKVSGGAEAVGRQTTVS
                     VVQSIFLVIVVDAAFSVLFSWLKI"
     gene            4611..5426
                     /locus_tag="PK144_10895"
     CDS             4611..5426
                     /locus_tag="PK144_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF66279.1"
                     /translation="MAEDLTPPPSPPPVGDAPPVIEIRGLGTRFGAQVVHEDLELTVR
                     RGEIVGLVGGSGSGKTTLLREVALLDAPSAGSVRLFGAETVGLDESEAFALRRRFGMM
                     FQHGALFSSLTVRENVAVPLHEHTTLPEAEIDRLAELKVALAGLPPGSGDKYPSQLSG
                     GMIKRAGVARALALDPDLLFLDEPTAGLDPVGAAALDELVLELRAALGLTVLIVTHDL
                     DTLWRITDRVAFLGERRVLACEPIESLSRRPHPLIRQYFCGPRARAAEAAAED"
     gene            5448..6371
                     /locus_tag="PK144_10900"
     CDS             5448..6371
                     /locus_tag="PK144_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNF66280.1"
                     /translation="MENKVNYTLVGVFVLALSAALIGTLLWLTVGTEEKQYDIYRLFF
                     RDSVAGLNPKAAVRLRGVAVGEVAGIALDRKQPDRVIVDLKIERGTPILESTKAVLAT
                     QGLTGIAAIELSVDNPNAPPLKRREDEPFPVIPTGPSLVQRLDNAVTQVLGDFGKLSN
                     RLQALLDQDNLDAVHDTLGNLRTLSAALAKQTATLDQVLRATADTAEAASGAAKEIRT
                     LGARLKTTVGLVDDTVRSFGQTARTLDTAVAGTRRDLQQFAGSAGPELAGLLAELRRM
                     TEVVERLGGELERNPRMLLFGRKNDNPGPGE"
     gene            6407..7042
                     /locus_tag="PK144_10905"
     CDS             6407..7042
                     /locus_tag="PK144_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNF66281.1"
                     /translation="MTRVRLTLLALLLGGCSAINLGSSPDAPRTIWLLSAVPAAAPAG
                     AARPAGVLLVDKPEARAGFDTPRMAYSRQPLAIDYYTRSEWADVPARMLQPLIVQTLA
                     QGGTWTAVLPAPNPGRADVRLTTNVLELVHDLSAGEPGVVRLRLRAVFTDVVDRRVLA
                     TREFAAVEPAASANAEGYAAAADRADARVLAGLAAFTAETFAPAPARVGRR"
     gene            7219..8421
                     /locus_tag="PK144_10910"
     CDS             7219..8421
                     /locus_tag="PK144_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF66282.1"
                     /translation="MSSIRMFHCRFSCRTAIRPWWPAALLGLLGLLAGGLPLAQAGTV
                     EPIRIGSVLTLSGPGAALGEPELKTLQLYVERLNAAGGLLGRPLRLIHVDDGGELAKV
                     DPAFRRLLDAERVDVLIGGTTSSASLAALPLVERAEVPFLSLAGSVLIVEPAKKWVFK
                     TAHTDRMACDKVFADLRARGLTRVALLSEASGYGRSGRFQCLARAAASGVQVVLDDSH
                     DPKEADFSAPLARIGASDAQALLVLGVGASSARITRAVHAAGLKLALYHTHSIGSKAF
                     IEAAGDAAEGVRFPAAALIIAAQLPEADPQKRVALEYTQAYTAAYGGSVSTFGGHAYD
                     ALMQWAEAVRRAGSVEPDAVRRALEATHGYVGTGGIVHYAPTDHLGLDATAFRMIEIR
                     NGDWRLID"
BASE COUNT         1222 a         3031 c         3038 g         1257 t
ORIGIN      
        1 cgttgccgga cggaagcaga gcatgcgcag cagcgccatc tcgaagccgc cgcgcggctc
       61 gggcgcgagc ggcaggtcgc ggcgcgcgtg cagcgcgatc tggtagcaga gctgcacgtc
      121 ctcggccgtg aacgcggcgg cgagacggac gagcgtatcg ctgtcgtaca gctcgtcgct
      181 ggcaccgggc acggcctgcg cgagcgcgac gcgctgcagc agcgacagca gctcggcgag
      241 cacgccgtcg taatccggcg cctcgcgatc gagccgctcg atcgccgcga gcacactggc
      301 cgcatcgccg ccggccaggg cttcggcgag gtgcacgacg tgctggcggt cgatcgtgcc
      361 gagcatcgcc gcggtgtcga gggcgccgac gcgaccggcg ccgaaggcga tggcctgatc
      421 gagcagcgac agcgcatcgc gcatcgagcc gtcgccggcg cgcgcgagtg cggcgagcgc
      481 ggccggttcg tgtgcgatgc cttcggcgtc gaggatgtcg gcgagccggc tctggatcag
      541 cgccgccggc agacgcttga ggctgaactg caggcagcgc gacagcacgg tgaccggcag
      601 cttctgcggg tcggtggtcg cgagcaggaa cttcacgtgc ggcggcggct cttcgagcgt
      661 cttcagcagc gcgttgaagc tgctggtgct cagcatgtgc acttcgtcga tcaggtagac
      721 cttgtagcga ccgcgcgccg gtgcgtactg gacgttttcg agcagctcgc gcgtgtcctc
      781 gaccttggtg cgcgaggccg catcgacctc gatcagatcg acgaagcggc cctcgtcgat
      841 ctcgcggcag gccgcacaga cgccgcaggg ctcggcggtg atgccggtct cgcagttcag
      901 cgacttggcg aggatgcgcg cgatcgtggt cttgccgacg ccgcgcgtgc cggtgaacag
      961 gaaggcgtga tgcaggcggc cgttggcgag cgcgttgccg agcgcgcgca cgacgtgctc
     1021 ctgcccgacc agttccgcga agcgcttcgg ccgccatttg cgcgccagaa cctgatggct
     1081 catctcgtgc cgcctgccgg gtggttcgcg tgggccgccg cgccctgcca tgcctgacgc
     1141 ggccggaaat gggtggtggc ccgtaccaga cgcaccccgg cgcccgcatc ggccgctacc
     1201 gttgctccct tccgggcctg gcggggttca cggcctagcg tcgcgagggg accgatacgg
     1261 gccaccatag aaaacgtgca gctcgaagac tgcgctcctg cgaccggccg gccgcgaggg
     1321 aggcgcagtc tagccgaagc gccggccgcg gggaaaccgc cgcaggcgca gtccgggaac
     1381 cagcttctac gctaggggcc gggcggtcgc agaccgggag cagcctcccg tccgcccgca
     1441 atcccatcac ccgagtgcag acgatgaagc tcgaaaccct cgcgatccac gccggctaca
     1501 gccccgaccc gaccacgaag gcggtcgcgg tgccgatcta ccagaccacg tcgtatgcgt
     1561 tcgacgacac gcagcacggc gcggacctgt tcgacctgaa ggtcgccggc aacatctaca
     1621 cgcgcatcat gaacccgacc acggcggtgc tggaggcgcg catggcggcg ctcgaaggcg
     1681 gcatcggcgc gctcggactc gcctccggac aggcggcgat cacctacgcg atccagacca
     1741 tcgccgaggc cggcgacaac atcgtcgcca ccggcacgct ctacggcggc acctacaacc
     1801 tgttcgcgca cacgctgccg cagtacggca tcgaggtgcg cttcgtcgat taccgcgatc
     1861 cggcctcggc ggcggcgctg atcgacggcc gcaccaaggc ggtgttctgc gagacgatcg
     1921 gcaatccgct cggcaacgtc gtcgacttcg aagccttctc gcaggttgcg cacgcgcacg
     1981 gcgtgccgct catcgtcgac aacacggtcg cgacgccgta tctgtgccgg ccgttcgagc
     2041 atggcgccga catcatcgtg cactcgctga ccaagtacct cggaggccac ggcacctgca
     2101 tcggcggcgt gctggtcgat tcggggaaat ttccctgggc cgagcacaag gaacgcttcc
     2161 gccgcctgaa cgagccggat gtctcctatc acggcgtgac ctacaccgag gcgctcggcg
     2221 cggcggccta catcggccgc gcgcgcgtcg tgccgctgcg caacatgggc gcggcgatct
     2281 cgccgttcaa cagcttcctg gtgctgcagg gcatcgagac gctggcgctg cgcatggacc
     2341 ggcactgcgc gaatgcgctc gcggtcgccg gattcctgcg cgatcacgcg gcggtcgagt
     2401 gggtcggcta tccgggcctg cccgacagcc cgttccggcc gctggtcgat cgctacatgg
     2461 gcggcaaggg ctcgggcctc gtgaccttcg gcgtcaaggg cggacgcgag gccggtgcgc
     2521 gcttccagga tgccttgcag cttgtgacgc ggctggtcaa catcggcgac gccaagagcc
     2581 tggcctgcca tccggcgagc accacgcacc gccagctcaa cgccgcggaa ctggcgaagg
     2641 ccggcgtcag cgaggacatg gtgcggctgt cgatcggcat cgagcacatc gacgacatcc
     2701 tcgccgacct cgaccaggcg ctggccgcga cgcgctgaag cgccgggggc gggccgccgg
     2761 acacaggcct tccagggaga ttccgaacac gatgctgcac cgtcacctcg tcgcctggct
     2821 gcgcagtttc ttcgtcagcg atttcggccg gccggcacgc ggtccggcgc cgctgttcgc
     2881 ggcgctcgcc ttcgtgctcg tcgtcatcgg cgtgctggtg ctgcagccct ggggcatgag
     2941 cagcagcgcc ggaccggcgc cggtcggggc gaccgaggac tgcacggtgc gctcggtgca
     3001 ggacggcgac accgtcacgc tcgcctgcaa cgctcaggag cggcgcgtgc ggctctgggg
     3061 catcgacgcc ccggaactcg gccagggcga ctgggggcgg ctcgcgcgca gcggcctgac
     3121 ggcgcggctg cgcaaggaga acgtgcgcct cgaagtgctg gacaccgacc agtacgggcg
     3181 cacggtcggg cgcatctggc tcgggaagac cgacatcggt ctcgaacagg tccacgccgg
     3241 gcgcgccgcc gtctatcgcg agttcaacaa cagctccgaa tacacgaccg ccgaagccga
     3301 ggcccggcgc gccgggcgcg gcatctggga agcccccggg gcgcagcaga cgccgtggct
     3361 gtggcggcgg ctgaatccgc gatgaatccg cgaggagggt ccgggcggaa tccgggcgga
     3421 gtccatgccg ggtccgtgct gaagcggtgc tgaatccgtg ccgatgcgcc tgccggcagc
     3481 cgccgtcgcg tggcttggcg cccgtgcggc gcccgtgaaa cactcgaagc catgacgact
     3541 gccgagctcg tgcttcccac ctgcaccgcc gacccgtccg ggggcgtgcg gctcgatggc
     3601 agctggacgc tcgccggctt tgcctgcggc ctgccgctgc tgccggaaaa gccggtgccg
     3661 cgcatcgacg ccggcggcct gaccgcgctc gacaccgccg gggcgctgca gatcctcgcg
     3721 ctcgaagccg gcagcgacac gcactgggac ggcctgaccg cagggcacgc gacgctgctc
     3781 gatctggtgc gcgcccaggc tgcgcgcgag cgcccgccgc cgccgccggt gccgggctgc
     3841 ctcgaagcac tcggccggcg cgcgacggcg cagctctgcg aggtgcggga tttcctcggt
     3901 ttcttcggcg agatgagcct cgcgttctgg cgcgcgctgc tcctgccgca ccggttccgc
     3961 tggacgccgt ttctcgccaa catccagagc gcggggctcg cggcgatgcc gatcgtcggg
     4021 ctgctgtcct tcctgctcgg cgtcgtcatc gcctatcagg gcggctcgca gctcgggcgc
     4081 tacggcgcca acatcttcat cgtcgagctg gtcagcctga cgatgctgcg cgagatgtcg
     4141 gtgatcatga ccgcgatcat cgtcgccggg cgcaccggct cggcgtggac cgcgcagatc
     4201 ggcacgatgc gcgtgaccga ggagatcgac gcgctgcgca ccatcggcat ccggccgatg
     4261 gacatgctgg tgatcccgaa ggtgttcgcg ctgatggtcg cgctgccgct gctgaccgtg
     4321 tacgccgatg cgatgggcgt gctcggcggc atggtgatgg ccgacctgca gctcggcgtg
     4381 agcttccagg agttcctgca gcgcttcccg caggcggtgt cggtgcgctc cttcctgatc
     4441 ggcgtcggca aggcgccggt gttcgcgctg ctgatcgcgc tggtcggctg ctatcagggc
     4501 ttcaaggtct cgggcggcgc cgaggcggtc ggccggcaga cgaccgtctc ggtcgtgcag
     4561 tcgatctttc tggtcatcgt cgtcgatgcg gcgttctcgg tgctgttctc gtggctgaag
     4621 atctgacgcc gccgccatca ccaccaccgg ttggcgacgc gccgccggtc atcgagatcc
     4681 gcggcctcgg cacgcgcttc ggtgcgcagg tcgtgcatga agacctcgaa ctgaccgtgc
     4741 gtcgcggcga gatcgtcggg ctggtcggcg gcagcggcag cggcaagacc acgctgctgc
     4801 gcgaggtcgc gctgctcgat gccccctcgg ccggcagcgt gcggctgttc ggcgccgaga
     4861 ccgtcggtct cgacgagagc gaagccttcg cgctgcgccg gcgcttcggc atgatgttcc
     4921 agcacggcgc gctgttctcg tcgctgaccg tgcgcgagaa cgtcgccgtg ccgctgcacg
     4981 agcacacgac gctgcccgag gccgagatcg accggctcgc ggagctgaag gtcgcgctcg
     5041 ccggcctgcc gccgggcagc ggcgacaagt atccgtcgca gctctcgggc ggcatgatca
     5101 agcgcgccgg cgtggcgcgc gcactcgcgc tcgatccgga cctgctgttc ctcgacgagc
     5161 cgaccgccgg cctcgacccg gtcggcgccg cggcgctcga tgaactggtg ctggaactgc
     5221 gtgccgcgct cggactcacg gtactgatcg tcacgcacga tctcgacacg ctctggcgca
     5281 tcaccgaccg cgttgccttc ctcggcgagc ggcgcgtgct cgcctgcgag ccgatcgaat
     5341 cgctgtcgcg gcgcccgcat ccgctgatcc gccagtactt ctgcggtccg cgcgcgcggg
     5401 ccgccgaggc agccgccgaa gactgagccg cgcgcaggag gcagccgttg gagaacaagg
     5461 tcaactacac gctggtcggg gttttcgtgc tggccctgtc ggcggcgctg atcggcacgc
     5521 tgctgtggct gacggtcggc accgaggaaa agcagtacga catctaccgg ctgttcttcc
     5581 gcgactcggt cgccggcctg aacccgaagg cggcggtgcg cctgcgcggc gtggcggtcg
     5641 gcgaggtcgc cggcatcgcg ctcgatcgca agcagcccga tcgcgtcatc gtcgatctga
     5701 agatcgagcg cggcacgccg atcctcgaaa gcacgaaggc cgtgctcgcg acccagggcc
     5761 tgaccggcat cgccgcgatc gagctgtcgg tcgacaaccc gaacgcaccg ccgctgaagc
     5821 gacgcgaaga cgagccgttt ccggtgatcc cgaccgggcc gtcgctggtg cagcgcctcg
     5881 acaacgccgt gacccaggtg ctcggggatt tcggcaagct gtcgaaccgg ctgcaggcgc
     5941 tgctcgatca ggacaacctc gatgccgttc acgacacgct cggcaacctg cgcacgctca
     6001 gtgcggcgct cgcgaagcag accgccacgc tcgatcaggt gctgcgcgcc accgccgata
     6061 ccgccgaggc cgcgagcggc gcggcgaagg agatccgcac cctcggcgcg cgcctgaaga
     6121 ccacggtcgg cctcgtcgat gacaccgtgc gcagcttcgg acagaccgca cgcacgctcg
     6181 acacggcggt cgccggcacc cggcgcgacc tgcagcagtt cgccggcagc gccggccccg
     6241 aactcgccgg cctgcttgcc gagcttcggc gcatgaccga ggtcgtcgag cgcctcggcg
     6301 gcgaacttga acgcaatccg cgcatgctgc tgttcgggcg caagaacgac aatccggggc
     6361 cgggcgaatg aatgccgccg gggcctgcat gaagcgagga ctgccgatga cccgtgtccg
     6421 cctcacgctg ctcgcgctgc tgctcggcgg ctgctcggcg atcaatctcg gcagctcgcc
     6481 ggatgcaccg cgcacgatct ggctgctgtc ggccgtgccg gcggccgcac cggccggcgc
     6541 ggcgcgcccg gccggcgtgc tgctggtcga caagcccgag gcccgcgccg gcttcgacac
     6601 gccgcgcatg gcgtattcgc gccagccgct ggcgatcgac tactacaccc gcagcgaatg
     6661 ggccgacgta ccggcgcgca tgctgcagcc gctgatcgtg cagacgctcg cgcagggcgg
     6721 cacctggacg gcggtgctgc cggccccgaa tccgggccgt gccgacgtgc gcctgacgac
     6781 gaacgtgctc gaactcgtgc acgatctgtc ggccggcgag ccgggcgtcg tgcggctgcg
     6841 cctgcgggcc gtgttcaccg atgtcgtcga ccggcgcgtg ctggcgacgc gcgaattcgc
     6901 cgccgtggag ccggcggcga gtgcgaatgc cgagggctac gcggcggcgg ccgatcgcgc
     6961 cgatgcccgc gtgctcgccg ggctcgcggc gttcacggcc gaaaccttcg cgccggcacc
     7021 ggcacgtgtt ggccggcgct gagcgccatc cggcatccgg catccgcaag cgcgcagtac
     7081 cgcgattccg ggcagtaccg cagtaccgca atgccttttc agccgtagcg aagaccagac
     7141 ccgaaacacg ggcaaatggc ggcgtgccag tgacgggcgc caaccatcca gggataccag
     7201 ggaaagagga gaaaaccgat gtccagcatt cgcatgttcc actgccggtt cagctgccgc
     7261 acagcgatcc ggccgtggtg gccggcagcg ctgctcggtc tgctcgggct gctcgctggc
     7321 ggcctgccgc tggcgcaggc cggcaccgtc gagccgatcc gcatcggttc ggtgctgacg
     7381 ctgagcggcc ccggcgccgc ccttggcgag ccggagctga agacgctgca gctctacgtc
     7441 gagcgcctga acgccgccgg cggtctgctc gggcggccgc tgcggctgat ccacgtcgat
     7501 gatggcggcg aactcgccaa ggtcgatccg gctttccggc gcctgctcga cgccgagcgt
     7561 gtcgatgtgc tgatcggcgg caccaccagc agcgcctcgc tggcggcgct gccgctggtc
     7621 gagcgcgccg aggtgccgtt cctgtcgctc gccggctcgg tactgatcgt cgagccggcc
     7681 aagaaatggg tgttcaagac cgcgcacacc gaccgcatgg cctgcgacaa ggtgttcgcc
     7741 gacctgcgcg cgcgcggcct gacgcgggtg gcgctgctgt cggaagcgag cggctacggc
     7801 cgttccgggc gcttccagtg tctggcgcgc gccgcggcca gcggcgtgca ggtcgtgctc
     7861 gacgacagtc acgatccgaa ggaagccgac ttctcggccc cgctcgcgcg catcggtgcg
     7921 agcgacgcgc aggcgctgct cgtgctcggc gtcggtgcca gctcggcccg catcacgcgc
     7981 gccgtacacg cggccggcct gaagctcgcg ctgtaccaca cgcattcgat cggctcgaag
     8041 gccttcatcg aggccgccgg tgacgcagcc gaaggcgtgc gctttccggc cgcggcgctg
     8101 atcatcgccg cccagctgcc ggaggccgat ccgcagaagc gcgtcgccct cgaatacacg
     8161 caggcctata ccgccgcgta tggcggcagc gtctcgacct tcggcggtca tgcctacgat
     8221 gcgctgatgc agtgggcgga ggccgtgcgt cgcgctggca gcgtcgagcc cgacgcggtg
     8281 cgccgggcgc tggaggcgac gcacggctac gtcggcaccg gcggcatcgt ccactacgcg
     8341 ccgaccgatc acctcgggct cgacgccacg gcattccgga tgatcgagat ccgcaacggt
     8401 gactggcggc tgatcgactg aagcggttgc cgcgacgctg cgcggcgctt tcagtccgcg
     8461 gccggccaga acggcggctg cgcgcgccag tgccgccagt gcctgcgcag cggctcccgc
     8521 aactcgcgag ccgccgccgc cgccaggg
//
LOCUS       DAOQIE010000163         8517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4230_length_8517_cov_6.231624, whole genome shotgun sequence.
ACCESSION   DAOQIE010000163 DAOQIE010000000
VERSION     DAOQIE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4230_length_8517_cov_6.231624"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /gene="ggt"
                     /locus_tag="PK144_10915"
     CDS             complement(<1..1610)
                     /gene="ggt"
                     /locus_tag="PK144_10915"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017786376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNF66283.1"
                     /translation="MRCPCLILAAALWLGGPVAVADAPVPAPEAASGWTPKTAVQARE
                     FMVAAAHPLAVETGRDVIRRGGSAADAAIAMQLVLGLVEPQSSGLGGGAFVLYWDAAA
                     GQVKSLDGRETAPMEADGRLFLDADGQPLAFLAAVIGGRSVGVPGTPKLLAELHRRWG
                     RLPWAELFAPAIRLAREGFPVSPRLAGAIAALGDGGSHRDGFGNDAARELFLHADGTP
                     LRAGERLVNPAYADTLATLAAEGVGPFYQGPIAEDIVRTVGSHAANPGRMSTGDLAAY
                     TVRERVPVCAPYRQWRVCGMGPPSSGGVTLLQMLGILERLPPPPAGQPPALAALHRFA
                     EAGRLAYADRERYLADPDYALVPVEGLLDPDYLEVRAGLFDAQRSLGRAAAGSPPGAP
                     SRGEARAAELPSTSHLVIVDRDGNAVSMTTTIENGFGSRLLVRGFLLNNELTDFAFAP
                     TGADGQPVANRVEPGKRPRSSMAPTLVFGPDGRLLLALGSPGGSSIINYVAKTLLAVL
                     DDHLDLQAAIDRPNFGSRNGPTELEAGTA"
     gene            complement(1671..2042)
                     /locus_tag="PK144_10920"
     CDS             complement(1671..2042)
                     /locus_tag="PK144_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF66284.1"
                     /translation="MSDRDPSVPELERFEPGPRYSEAVAAGGLVFLAGQVPEDPEAGI
                     TQQAAEVLAAVERALALAGSTRARLVSATIYLREMADYAAFNAVWDAWLLPHPAPARA
                     CVQALLADPRWRVEVQVVAAR"
     gene            complement(2117..2353)
                     /locus_tag="PK144_10925"
     CDS             complement(2117..2353)
                     /locus_tag="PK144_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66285.1"
                     /translation="MEDLKTLKHTGRTPSLDPPVLQRLRELHAALARLPGGRDVHDAL
                     DPLLRGLSPDQRERMREQITGRIFSRRSSSSRGL"
     gene            complement(2371..3135)
                     /locus_tag="PK144_10930"
     CDS             complement(2371..3135)
                     /locus_tag="PK144_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF66286.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAIAAARGRRTLVVDLDPQANSS
                     HYLLGGAADGEGPTVADFFDDMLSFKFNPPGPEHFARPTPFVNLFVMPSQGRLEELGP
                     KLESRYKMFKLREALESLRGFDEIFIDTPPAVNFFTRSALIAAERCLIPFDCDSFSRR
                     ALYLLLDNVREMQADHNRGLQVEGIVVNQFQSQARLPRQLVEELRAEGLPVLDAFLST
                     SVKIRESHERALPMLHLDPRHKLTQEFEALYGLLAA"
     gene            complement(3249..4124)
                     /locus_tag="PK144_10935"
     CDS             complement(3249..4124)
                     /locus_tag="PK144_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNF66287.1"
                     /translation="MPLPLLLALLLSFPIPARADLIVTDDTGRAVQLPAPARRIVAVA
                     PHAAELLHAAGAGAALVGVVQYADFPPAVRTLPSVGSYTGFDLEALLALKPDLVIGWD
                     SGNPPAQLARIEALGIPVFRTEARVADDIATDIEKLGELAGTAAVATQAAAAFRQRLA
                     QLRREHAGRRPVRVFYQVWARPLVTLNGEHPVSHVLADCGGVNVFAALPALAPQIDEE
                     AVLAADPEVIIGGGMDGERPEWLEDWRRRWPQLAAVRGGHLYAVPPDWLHRSGPRLLD
                     GMERVCAQLDAARGG"
     assembly_gap    4253..4352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4353..4709)
                     /locus_tag="PK144_10940"
     CDS             complement(<4353..4709)
                     /locus_tag="PK144_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66288.1"
                     /translation="MSPCAPLTLQHPLSPELAGALMALAARLDGRRWFLIGAGARDLL
                     LAECPHARQPLRLLFQQHLPIDLVPWGHQDANGSIAWPPDFAVRMSVIGYDEAYAANP
                     LMPAAAGPDERLDCARR"
     gene            complement(4706..5581)
                     /locus_tag="PK144_10945"
     CDS             complement(4706..5581)
                     /locus_tag="PK144_10945"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HNF66289.1"
                     /translation="MKRAETVLARPQLPGRLAAEARLLLCERVTAAQAGRLRAAGVRY
                     LDAAGNAWLHDGTLFVLIEGREPVTSIRSRMPRLLRPAGLKLLFLLLREPAAVAATYR
                     ELAARSGISLGAVGQVMQDLRAAGHLLSEQGRARLVRRRTLLDRWVPAYQENLRPRLV
                     FGDYTPLAAGTDWQAFTPAPGVDYWGGDAAADRLTHYLASRAPSLYTRETLPSRLALR
                     QRWRAARPEDARAGLVIRVLRAFWPLEADDPRHPGLVPPLLVYADLLGNGDDRSREAA
                     ARVYDAYLAVDLDAP"
     gene            complement(5843..6841)
                     /gene="cobD"
                     /locus_tag="PK144_10950"
     CDS             complement(5843..6841)
                     /gene="cobD"
                     /locus_tag="PK144_10950"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNF66290.1"
                     /translation="MLEHGGRLNAAAARYRIDRADWLDLSTGINPDGWPVPQLPAEVW
                     QRLPEDDDALPIAVEQHYGRPGLPVAGSQAALATLPRLRAACRVGVIGPTYGEHAHAW
                     QRAGHEVRTLTVGAVEAALGQLDVLVAVNPDNPTGRLLASSTLLGWHRRLAARGGWLL
                     VDEAYMDAEPSESLLAVREQPGLIVLRSLGKFWGLAGVRAGFVFAEADLRAALAGLLG
                     PWSVSGPARAVAVRALADTHWQDHARACLGHAARRFARLLTRHGLPPAGLTALFQWVP
                     HPAAAELHEGLARRGVLVRHYAEPGGLRFGLPPDEAALRRLELALAGVRAERLGRR"
     gene            complement(7023..7943)
                     /gene="cbiB"
                     /locus_tag="PK144_10955"
     CDS             complement(7023..7943)
                     /gene="cbiB"
                     /locus_tag="PK144_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNF66291.1"
                     /translation="MPMSSVSATVLVPALAYLLDRLLGEPPLRWHPLVWFGRWAGALE
                     RRLYRDSIRRGGLVWMLAVLPPTLLAGGLGALPGFGPWLGAGLLMLALGARSLDEHAQ
                     AVAVPLAAGELPAARQQLARIVSRDTAALDEAGIARAAVESVLENGNDAICGALFWYA
                     LAGPAGAVGYRLANTLDAMWGYRTARHERFGKVAARIDDALNWLPARLCAASYALAGD
                     ARTAWRCWRTQARHWDSPNAGPVMAAGAGALGLALGGPASYGGHLETRPALGAGRAPR
                     AAGIGRACALVRRASGLWVLLAMLSSGLRG"
     gene            8052..>8517
                     /locus_tag="PK144_10960"
     CDS             8052..>8517
                     /locus_tag="PK144_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF66292.1"
                     /translation="MSARTLMIQGTASDAGKSTLVAALCRLLARRGVAVAPMKPQNMA
                     LNSAVTVDGGEIGRAQALQAQAARLDAHTDMNPVLLKPNTDTGAQVIVHGRAIGTMDA
                     VAYHAYKPVALAAVLASHARLAARYEHVIVEGAGSPAEINLRAGDIANMGFAE"
BASE COUNT         1259 a         3089 c         3053 g         1016 t
ORIGIN      
        1 gcggtgccgg cctccagctc ggtcgggccg ttgcggctgc cgaagttcgg ccggtcgatc
       61 gccgcctgca ggtcgaggtg gtcatcgagc acggccagca gcgtcttggc gacgtaattg
      121 atgatgctgc tgccgcccgg tgagccgagc gcgagcagca gccggccgtc cgggccgaac
      181 accagcgtcg gcgccatcga cgagcgcggg cgcttgccgg gctcaacgcg gttggcgacc
      241 ggctggccgt cggcaccggt cggcgcgaac gcgaagtcgg tcagctcgtt gttgagcagg
      301 aagccgcgca ccagcagccg gctgccgaag ccgttctcga tcgtcgtggt catgctgacg
      361 gcgttgccgt cacgatcgac gatgaccaga tgcgaggtcg acggcagttc ggcggcacgc
      421 gcctcgccgc gcgacggcgc gccgggcgga ctgccggccg cagcgcggcc gagcgagcgc
      481 tgcgcatcga acagcccggc gcgcacttcg aggtaatccg gatcgagcag gccctcgacc
      541 ggcaccagcg cgtagtccgg atcggccaga tagcgttcgc gatcggcata ggcgagccgc
      601 ccggcctcgg cgaagcggtg cagcgccgcg agcgccggcg gctgcccggc cgggggcggc
      661 ggcaggcgtt cgaggatgcc gagcatctgc agcagcgtca cgccgcccga actcggcggc
      721 cccatgccgc acacccgcca ctgccggtac ggcgcgcaca ccggcacgcg ctcgcgcacc
      781 gtgtatgcgg ccagatcgcc cgtgctcatg cgcccgggat tggccgcgtg cgagccgacc
      841 gtgcgcacga tgtcctcggc gatcggcccc tgatagaacg gcccgacgcc ttcggcggcc
      901 agcgtcgcca gcgtgtcggc gtaggccgga ttcacgagcc gctcgccggc gcgcagcggc
      961 gtgccgtcgg cgtgcaggaa cagctcgcgc gcggcatcgt tgccgaagcc gtcgcggtga
     1021 ctgccgccat cgccgagcgc ggcgatggcg ccggcgagcc gcggcgacac cgggaagccc
     1081 tcgcgcgcca gccggatcgc cggcgcgaac agctcggccc acggcagccg gccccagcgc
     1141 cggtgcagct cggcgagcag cttcggcgtg ccgggcacgc cgaccgaacg gccgccgatc
     1201 acggcagcga ggaaggcgag cggctggccg tcggcatcga ggaacagccg gccgtcggcc
     1261 tccatcggtg cggtctcgcg gccgtcgagc gacttcacct gcccggccgc ggcatcccag
     1321 tacagcacga aggcgccgcc gccgagcccc gaggactgcg gctcgacgag gccgagcacc
     1381 agctgcatcg cgatcgcggc atcggcggcc gaaccgccgc ggcggatcac gtcgcgaccg
     1441 gtctcgaccg ccagcggatg cgcggcggcg accatgaact cgcgcgcctg cacggccgtc
     1501 ttcggcgtcc agccgctcgc ggcctcgggc gccggcaccg gcgcatcggc aacggcgacc
     1561 gggccgccga gccagagcgc ggcggcgagg atcaggcacg gacagcgcat cgacaatctc
     1621 cggaacatgc cagcgaaagg caggaaatga acggggaacc acccgcggct tcagcgcgcc
     1681 gcaaccacct gcacctcgac gcgccagcgc ggatcggcga gcagcgcctg cacgcaggcg
     1741 cgcgccggtg ccggatgcgg caggagccac gcatcccaga cggcgttgaa cgccgcgtag
     1801 tcggccatct cgcgcaggta gatggtcgcc gacaccagcc gcgcgcgcgt gctgccggcc
     1861 agcgccagcg cccgctcgac ggcggcgagc acctcggcgg cctgctgcgt gatgccggcc
     1921 tccgggtcct cgggcacctg accggcgagg aagaccagcc caccggccgc cacggcctcg
     1981 gaatagcgcg ggccgggctc aaaacgctcg agctccggca cggacggatc gcgatcggac
     2041 acggtgttcc cctcgttcgc agccccggcg gaacgcggcg caccgacccg gggctgcggg
     2101 cggcgcgcct cgggcctcac agcccgcgtg atgatgacga gcgccggctg aagatgcgcc
     2161 cggtgatctg ctcgcgcatg cgctcgcgct ggtccggcga caggccgcgc agcagcggat
     2221 cgagcgcgtc gtgaacatca cggccgccgg gcagccgcgc gagtgccgcg tgcaactcgc
     2281 gcaggcgctg cagtaccggc ggatcgaggc tgggcgtccg cccggtgtgc ttcagggtct
     2341 tgaggtcttc catggccgcc gtgtgcaagc tcaggccgcc agcaggccgt acaaggcctc
     2401 gaattcctgc gtgagcttgt gacgcgggtc gaggtgcagc atcggcagtg cgcgttcgtg
     2461 cgattcgcgg atcttgaccg aggtcgacag gaaggcatcg agcaccggca gaccctcggc
     2521 gcgcagttcc tcgaccagct ggcgcggcag ccgcgcctgc gactggaact ggttgacgac
     2581 gatgccctcg acctgcaggc cgcggttgtg gtcggcctgc atctcgcgga cgttgtcgag
     2641 cagcagatac agcgcgcggc gcgagaaact gtcgcagtca aacgggatca ggcagcgctc
     2701 ggcggcgatc agcgccgaac gcgtgaagaa gttcacggcc ggcggcgtgt cgatgaagat
     2761 ctcgtcgaag ccgcgcaggc tttcgagcgc ctcgcgcagc ttgaacatct tgtagcgcga
     2821 ttcgagcttg ggcccgagtt cctccagccg gccctgcgag ggcatcacga acaggttcac
     2881 gaacggcgtc ggccgcgcga agtgttcggg tccgggcggg ttgaatttga acgacagcat
     2941 gtcatcgaag aaatccgcga ccgtcggccc ctcgccgtcg gcggccccgc cgagcaggta
     3001 gtgcgaggaa ttggcctgcg gatcgagatc gacgaccagc gtgcgccggc cgcgggccgc
     3061 ggcgatcgcc gcgagattgc aggtgatcgt cgacttgccg accccgcctt tctggttgaa
     3121 cacgacgcga cgcatgatct ttgtggtcct tcccccgggt ggtgcgccag tttaacgaac
     3181 cgtgacaggg aaattccgca ttcgttgcgc tgcgtcaagg ccggaccggc cggccggcag
     3241 accgccgttc agccgccgcg cgccgcatcg agctgcgcgc agacccgctc catgccatcg
     3301 agcaggcgcg gcccggagcg gtgcagccag tccggcggca ccgcgtacag atgcccgccc
     3361 cgcacggccg ccagttgcgg ccagcgccgg cgccagtcct ccagccactc cgggcgctcg
     3421 ccgtccatgc cgccgccgat gatgacctcg ggatcggcgg cgagcacggc ttcctcgtcg
     3481 atctgcggcg ccagtgccgg cagcgccgcg aacacgttga cgccaccgca gtcggcgagc
     3541 acgtggctga ccggatgctc gccgttcagc gtcaccagcg gcctggccca gacctgatag
     3601 aacacgcgca ccggccgccg cccggcatgc tcgcgacgca gctgcgcgag ccgctgccgg
     3661 aacgccgccg ccgcctgcgt cgccaccgcc gcggtgccgg ccagctcgcc gagcttctcg
     3721 atgtcggtgg cgatgtcatc ggcgacgcgc gcctcggtgc ggaacaccgg aatgccgagc
     3781 gcctcgatgc gcgcgagctg cgccggcgga ttgccgctgt cccagccgat caccagatcg
     3841 ggcttcagcg ccagcagcgc ttcgaggtcg aagccggtat acgaaccgac gctcggcagc
     3901 gtgcgcaccg ccggcggaaa atccgcgtac tgcacgaccc cgaccagcgc cgccccggcg
     3961 ccggcggcat ggagcagctc ggcggcatgc ggcgccaccg caacgatccg ccgcgccggc
     4021 gccggcaact gcacggcacg gccggtgtcg tcggtgacga tcagatcggc acgggcggga
     4081 atggggaagc tcagcaggag cgcgagcagc agcggcagag gcatgtcagg tgcgtccgtg
     4141 tgcagggtcg atgtgcggga gtgcatccgc gatgccgtcg gcataggcct gcagcaggcg
     4201 ccgtgcgcaa tccagccgct catccggccc cgcggcggcc ggcatcagcg gannnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgccgtg cgcaatccag ccgctcatcc
     4381 ggccccgcgg cggccggcat cagcggattt gccgcatagg cctcgtcgta gccgatgacg
     4441 ctcatgcgca cggcgaagtc cggcggccag gcgatgctgc cgttcgcatc ctgatggccc
     4501 cagggcacga gatcgattgg cagatgctgc tggaacagca gccgcagcgg ctgccgggcg
     4561 tgcggacact cggcaagcag caggtcacgg gcgccggcgc cgatcaggaa ccagcgccgg
     4621 ccgtcgagtc gggcggccag cgccatcagt gccccggcca gttccgggga cagcggatgc
     4681 tgcagcgtca gcggcgcaca cggactcacg gcgcgtccag atcgacggcc agatacgcgt
     4741 cgtacaccct ggcggccgcc tcccgggacc ggtcatcccc gttgccgagc agatcggcgt
     4801 agacgagcag gggcggcacc agccccggat gacgcggatc atcggcctcc agcggccaga
     4861 aggcccgcag cacgcggatg accagaccgg cgcgggcatc ttccggccgc gctgcacgcc
     4921 agcgctgacg cagcgcgagc cgggaaggca gggtttcgcg ggtgtacagg ctcggggccc
     4981 gggacgccag atagtgcgtc agccggtcgg cagccgcatc gccaccccag taatcaacgc
     5041 ccggagccgg cgtgaacgcc tgccagtcgg tcccggctgc cagcggggtg tagtcgccga
     5101 acacgagtct tggccgcagg ttttcctgat aggccgggac ccagcgatcc agcagggtcc
     5161 gccggcgcac gagccgcgcg cggccctgtt cgctgagcag atgaccggcg gcgcgcaggt
     5221 cctgcatcac ctgtccgacc gcgccgagcg agatgccgct gcgcgctgcc agttcgcgat
     5281 aggtcgctgc gacggccgcc ggctcgcgca gcagcaggaa cagcagcttc agtccggccg
     5341 gacgcagcag acgcggcatc cggctgcgga tcgaagtcac cggctcgcgt ccctcgatca
     5401 gcacgaacag cgtgccgtcg tgcagccagg cgttgccggc agcatccaga tagcgcaccc
     5461 cggcagcccg cagtcgcccg gcctgggccg cggtcacacg ctcgcagagc aacagccgtg
     5521 cttccgcggc gagtcgcccg ggcagctgcg gacgggccag cacggtttcc gcccgcttca
     5581 tttccacctc gaagcgatgc ccgtcgacct gcaggtgacc atcgggctcg ccggccgtcg
     5641 tggccgaccc gatcccgcgg tggaattcga ccgtcaggcc ggtcagccgg cgcagggcac
     5701 tggctgcgtg ctgaggccag tctgcgcttg tcgtcgggtc cggcgtcatc tgttcatttt
     5761 ttcgctgcgt tcacgaacaa tgaacgtagc agaaaaatga acaagaacgg caagccttcg
     5821 gccggatgcg ccggccggtg cctcaacgcc gccccagccg ctcggcgcgc acgccggcca
     5881 gcgccagttc cagccggcgc agcgcggcct cgtccggcgg caggccgaag cgcaggccgc
     5941 cgggttcggc gtagtggcgc accagcacgc cgcggcgggc gaggccttcg tgcagttcgg
     6001 ccgccgccgg gtgcggcacc cactggaaca gcgcggtcag cccggccggc ggcaggccgt
     6061 ggcgggtcag caggcgcgcg aagcgccggg cggcgtggcc gaggcaggcg cgggcgtggt
     6121 cctgccagtg cgtgtcggcg agcgcccgga cggcaaccgc gcgtgccggg ccgctgacgc
     6181 tccacgggcc cagcagcccg gcgagggcgg cgcgcaggtc ggcctcggcg aacacgaagc
     6241 cggcgcgcac gccggccagg ccccagaact tgccgagcga gcgcagcacg atcaggccgg
     6301 gctgctcacg cacggcgagc aggctctcgc tcggctcggc gtccatgtag gcctcatcga
     6361 ccagcagcca gccgccgcgc gcggccagtc gccggtgcca gccgagcagc gtgctcgaag
     6421 cgagcagccg gccggtcggg ttgtcggggt tgacggcgac gagcacgtcg agctgcccga
     6481 gcgcggcctc gaccgcgccg accgtcagcg tgcgcacctc gtggccggcg cgctgccagg
     6541 cgtgcgcgtg ctcgccgtag gtcgggccga tcacgccgac ccggcaggct gcgcgcagcc
     6601 gcggcagcgt cgccagcgcg gcctgcgaac cggcgaccgg cagccccggc cggccgtagt
     6661 gctgctcgac ggcgatgggc agcgcatcgt cgtcctccgg caggcgctgc cagacctcgg
     6721 ccggcagttg gggcaccggc cagccgtccg ggttgatgcc ggtcgacagg tcgagccagt
     6781 cggcacggtc aatccggtag cgcgcggcag cagcattcag ccggccgccg tgttcgagca
     6841 gcatcaagat cggggtctcc gcaggtcggg cgcgacggtg cggacgctcc ggcgcggtcg
     6901 ggggacgcat gataccgcca gagccggcac gcgccttgct taacatcaag acagctttgt
     6961 cggagcggga aaaattcgtt acacccctgc cgcgcagggc cgcggtaaag gcgcgccggc
     7021 gctcagccgc gcaggccgga gctcagcatc gcgagcagca cccacagccc gcttgcgcgc
     7081 cgcaccagcg cgcaggcccg gccgatgccg gcggcccgcg gcgcccgccc ggcaccgagc
     7141 gccggacgcg tttcgaggtg accgccgtag ctcgccggac cgccgagcgc gaggccgagc
     7201 gcaccggccc cggccgccat gaccgggccg gcgttcgggc tgtcccagtg ccgggcctgc
     7261 gtgcgccagc agcgccaggc cgtgcgtgca tcgccggcga gggcgtagct cgccgcgcac
     7321 agccgcgccg gcagccagtt cagcgcgtcg tcgatgcgtg ccgcgacctt gccgaagcgc
     7381 tcgtggcgcg ccgtccggta gccccacatc gcatcgagcg tgttcgccag ccggtagccg
     7441 acggcgccgg ccggcccggc cagcgcgtac cagaacagcg cgccgcagat cgcgtcgttg
     7501 ccgttttcca gcaccgattc gacggccgcg cgggcgatgc cggcttcatc gagcgcggcc
     7561 gtatcgcgcg agacgatgcg cgcgagctgc tgccgcgccg ccggcagctc gccggcggcg
     7621 agcggcacgg ccacggcctg cgcgtgctcg tcgaggctgc gcgcgccgag cgcgagcatc
     7681 agcagcccgg cgccgagcca cggcccgaag cccggcagcg ccccgagccc gccggccagc
     7741 agcgtcggcg gcagcaccgc gagcatccag accagcccgc cgcgccggat cgaatcccgg
     7801 tacagccggc gttcgagcgc accggcccag cgaccgaacc agaccagcgg gtgccagcgc
     7861 agcggcggtt cgccgagcag gcggtcgagg aggtacgcga gcgcgggcac gagcacggtg
     7921 gcggagacag aagacatcgg cattccgggg cgggcggtct gggggcgcgc gagtgtagcc
     7981 acggcgcagc ggcccgctac actcgcgcgc cccttcaccg ccctccccga catgccttcg
     8041 agagcccgcc gatgagcgcc cgcacgctga tgatccaggg caccgcctcc gatgccggca
     8101 agagcacgct ggtcgccgcg ctgtgccggc tgctggcgcg ccgcggtgtt gcggtcgcgc
     8161 cgatgaagcc gcagaacatg gcgctcaaca gtgccgtcac cgtcgatggt ggcgaaatcg
     8221 gccgcgcgca ggcgctgcag gcgcaggcgg cacgcctcga tgcgcacacc gacatgaacc
     8281 cggtgctgct gaaaccgaat accgacaccg gcgcgcaggt catcgtccac ggccgcgcca
     8341 tcggcacgat ggatgccgtc gcctaccacg cctacaagcc ggtggcactc gcggccgtgc
     8401 tcgcctcgca cgcgcggctg gcggcgcgct acgaacacgt catcgtcgag ggtgccggct
     8461 cgccggccga gatcaacctg cgcgccggcg acatcgccaa catgggcttc gccgagg
//
LOCUS       DAOQIE010000164         8464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4270_length_8464_cov_6.867166, whole genome shotgun sequence.
ACCESSION   DAOQIE010000164 DAOQIE010000000
VERSION     DAOQIE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_8464_cov_6.867166"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PK144_10965"
     CDS             complement(<1..951)
                     /locus_tag="PK144_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF66293.1"
                     /translation="MTEPFPIPGPPPAPDWYTGSRAHWRDASPELPEPLARSLDEPED
                     YLPGTALVAAVNVALTLGQPLLLTGEPGVGKTCLAANVASELALGTPLKCEVRSTTEA
                     GDLFYTFDAMAQFRDAHAGADAPSARWLDKPGRPGGAAPEAAPPVGVRRYLALQGLGL
                     AILLTRTPDDALVRRYWDPVRNDGPFRGPCRSVVLIDEIDKAPRDVPNDLLNQIELAY
                     FRIREDDNRRIDADPALWPIVIMTSNSEKHLPDAFLRRCVYHHIEFPDAVTLARIVDA
                     RLARVTVRERREPLAPTLVAEALQLFAALRSAPALRRRPGT"
     gene            complement(948..2441)
                     /locus_tag="PK144_10970"
     CDS             complement(948..2441)
                     /locus_tag="PK144_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNF66294.1"
                     /translation="MDERDLGPVARIATAGGRYVGVGLHVRPGGHVLSCAHVVADALG
                     CPDAQFRDEPPAGSVRVNFPFGPAPAAGYTACVADGGWQRNRRPDEAEEPSGRVDIAV
                     LRLDAPAPGVPCADFTADGWRWTGDGDAWTWGLPDGSDAGIPTRCTRLGPRTNGRTLL
                     DSSARNTVNAAIRITAGFSGGGVWLDRERRFAGLVATEAGGVAAEMIPARVLRAVWPA
                     LDPAATTPVAGSDPRDLLCLLDRTAACATLYRTLGAALPPRAPLAFFIDGDKRDWHDG
                     FVAHLRIAGRTQPLPEPVRGAAELLAALEPWPLRWEPDLGLDGLYAALVHALGLPAAT
                     APDASTLAAALAGRGGGLLLRYDIECEYWGLGAAERRLLRAWLDLLFGLGEPAQPLVV
                     LLCLRRTPAPASLAARLRRWWGRRFAQPAHARVSSIALGPVGTQDVCDWLEFTVRPAA
                     RHRARELDALRLELERELGYRNDLSMDHVLGCERLLRFLATDGSPAP"
     gene            complement(2463..2828)
                     /locus_tag="PK144_10975"
     CDS             complement(2463..2828)
                     /locus_tag="PK144_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HNF66295.1"
                     /translation="MRTGDTKIVVFTLASGESVPAEVEDDLVGLSFRPVSRAPDPGEL
                     PRSVQEALARLQPLADAVLDTLSGLTARPDQVEVQFGIKLSAAAGAVIAKAGSEANFA
                     VKLNWKRAEPVPRKRYGKR"
     gene            complement(2925..3227)
                     /locus_tag="PK144_10980"
     CDS             complement(2925..3227)
                     /locus_tag="PK144_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66296.1"
                     /translation="MRTALVSLVLTTLLAGVPLADAVAWTRHGSSSGPHGSSSVDASG
                     SCADRSCSRSVTRTGPAGRTSTHQGEAGCADGSCSGSSTTTGPRGATRTRSGSLSR"
     gene            3539..4330
                     /locus_tag="PK144_10985"
     CDS             3539..4330
                     /locus_tag="PK144_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF66297.1"
                     /translation="MNDTTGAARSAPPRVGLFVTCLADTIRPSVAFATVKLLEDAGCR
                     VEAPASQTCCGQPAFNSGDRADARALAEQTIAAFEGFDYVVGPSGSCLGMLRHYPELF
                     ETGSPWHARAERLAAKCFEITSFLVDVLGVERVQARYDGTVTYHDSCSGLRELGVKAQ
                     PRKLLATVEGLTLTEMNENEVCCGFGGTFCIKYPDISNRMVTEKVANIRASGAGTLLA
                     GDLGCLMNMAGKLKRQGSDVRVWHVAEVLAGMTDGAPIADAAPSR"
     gene            4349..5779
                     /locus_tag="PK144_10990"
     CDS             4349..5779
                     /locus_tag="PK144_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNF66298.1"
                     /translation="MQITSPRFKQNAHEALGDAALQRALGNLKAGFPGKRAAAVARMP
                     EFEQLRDAARDLKNHILEHLDFYLETFEANVKRRGGHVHWARDAEEARAIVLDICRKA
                     GAKTVTKGKSMITEEIGLNDHLAANGIEPIETDLGEYIIQLRGEHPSHIIAPAIHVNL
                     DQVSDAFHAHHGKYGKTERQTEPRALMNEAREILRDKFIAADVGITGANFCIAETGTT
                     VIVTNEGNGDLTQTLPRVHVAVTSIEKVVPTLEDATLLHRLLSRSATGQEIEVYGTWS
                     SGPRRPEDPDGPAEYHVVLLDNGRSNMLGNEFREMLRCIRCSACMNHCPVYGAIGGHA
                     YGWVYPGPMGSVLTPAFIGVEQAGNLPNASTFCGRCESVCPVRIPLPKLMRHWRAREF
                     EKHLTPQGFRFGLGVWAWLARRPKLYRLATQVATRVLKPLGGKTNRIGSLPLAGGWTA
                     WRDLPAPQGRTFFEQWKDRQQAGGAK"
     gene            5776..6450
                     /locus_tag="PK144_10995"
     CDS             5776..6450
                     /locus_tag="PK144_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNF66299.1"
                     /translation="MSGREAILGNIRKGLKRSGPLAGTALELCQTRLREHRPNLIPAR
                     AQLPHAGQVELFVQMAQEASATVARVTERAAVPAEVARYLADTGFAAEVIVAPDASIA
                     GLDWAAAGVATEARHARNGDRATVAPAFAGIAETGTLMLLAGPQSPTTLNFLPDAHIV
                     VLDAARIVGPYEDAWAMLRSERGEGWMPRVVNWITGPSRSADIEQKLQMGAHGPIRLH
                     IVLIGD"
     gene            complement(6536..7309)
                     /locus_tag="PK144_11000"
     CDS             complement(6536..7309)
                     /locus_tag="PK144_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66300.1"
                     /translation="MQYGLLIVEGQHDAAVIAKLLSDFNLKTIQNEKKLASYWRPLYA
                     NLKFPQGGDLLKRMDVPIFVENNTHSIAIRTADSDTNIASALEATLSGLGSKQDQLQA
                     IGIVCDADQQTASARMSKLCAALAGCNLTFAASTPGVVHAGPPRTGIMVFPDNLGPGT
                     LETLLEDCAATHYPQLQVLACNYMTDGAGHLDESERRNLEKGANRQKATIAVIGALLQ
                     PGASIAVTLRQDRWLTGSALAQPRIAALRTFLKDLLDLP"
     gene            complement(7309..8376)
                     /locus_tag="PK144_11005"
     CDS             complement(7309..8376)
                     /locus_tag="PK144_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF66301.1"
                     /translation="MRHLDSLTIGAFRGLRDVKLEGLGSVNVLVGDNNSGKTSVLEAL
                     ALFARPFDDKTWLASASLREIGGPGFLLSDAVNWFFPHSAERDEHSPVRLSGFASGKK
                     EASRVEASIRLVNATDPDVAGLVMPRRYVDVSRFLDENNAENATLGFWGSNWSVDRGD
                     PEPKMLPLRLLSSVAHRLTVFQTVEISSVLQKNDKKPVLDALQLFDQDIIDFLLLTDG
                     DGKNSRPYLRHRRLGPKSPAPLSIFGDGLRRAFSIALSLHSARHGLLLLDEIETAIHP
                     TALTTFFGWLIKVSRQSKIQLFCTTHSLEAVDALMAAETDHPGDIVVHRLEHTDNGTV
                     ATRIRGELLREMRYDFGMDVR"
BASE COUNT         1339 a         2877 c         2948 g         1300 t
ORIGIN      
        1 cgtgccgggc cggcggcgca gcgccggcgc gctgcgcagc gccgcgaaca gctgcagggc
       61 ctcggccacc agcgtcgggg ccagcggctc gcggcgctcg cggaccgtga cgcgcgcgag
      121 ccgggcatcg acgatgcgtg cgagcgtcac ggcgtccggg aactcgatgt ggtggtagac
      181 gcagcggcgc aggaaggcat cgggcagatg cttttcggag ttgctggtca tgatcacgat
      241 cggccacagc gccgggtcgg cgtcgatgcg ccggttgtcg tcctcgcgga tgcggaagta
      301 cgcgagttcg atctggttca gcaggtcgtt cggcacgtcg cgcggcgcct tgtcgatctc
      361 gtcgatcagc acgaccgagc ggcacgggcc gcggaacggc ccgtcgttgc gcaccggatc
      421 ccagtaacgg cgtaccagcg catcgtccgg cgtgcgcgtc agcaggatcg cgagtccgag
      481 gccctgcagc gcgaggtagc ggcgcacgcc gacgggcggc gcggcctcgg gggctgcgcc
      541 gccggggcgg ccgggtttgt cgagccagcg tgcagaagga gcgtccgcac ccgcgtgtgc
      601 gtcgcgaaac tgcgccatcg cgtcgaacgt gtagaacagg tctccggcct cggtcgtcga
      661 gcgcacctcg cacttcagcg gggtgccgag cgcgagttcg cttgcgacat tggccgcgag
      721 acaggtcttg ccgacgccgg gctcgccggt cagcagcagc ggctggccga gcgtcagcgc
      781 gacgttgacg gcggcgacca gcgccgtccc cggcaggtaa tcctcgggct cgtcgaggct
      841 gcgcgcgagc ggttcgggca attcggggga tgcgtcgcgc cagtgcgcgc ggctgccggt
      901 gtaccagtcg ggggcgggcg gcgggccggg gatcgggaag ggctcggtca tggggcaggg
      961 ctcccgtcgg tcgcgagaaa gcgcaggagc cgctcgcagc cgagtacgtg gtccatcgac
     1021 agatcgttgc ggtagccgag ttcgcgctcc agttcgagcc gcagcgcgtc gagctcgcgg
     1081 gcacggtggc gtgcggccgg tcgcaccgtg aactccagcc agtcgcacac gtcctgggtg
     1141 ccgaccggcc cgagcgcgat gctcgacaca cgcgcgtgcg cgggctgcgc gaagcgccgg
     1201 ccccaccagc ggcgcagccg cgccgccagc gaggccggcg ccggcgtgcg gcgcaggcac
     1261 agcagcacga cgagcggctg cgccggctcg ccgagcccga acagcaggtc gagccacgcg
     1321 cgcagcagcc gccgctcggc cgcgcccagt ccccagtact cgcattcgat gtcgtagcgc
     1381 agcagcaggc cgccgccacg gccggcgagc gccgcggcga gcgtgcttgc gtcgggcgcc
     1441 gtggctgcag gcagcccgag cgcgtgcacg agggcggcgt acagcccgtc gaggccgaga
     1501 tcgggctccc agcgcagcgg ccagggttcg agcgcggcca gcagctcggc cgcgccgcgc
     1561 accggctcgg gcagcggctg cgtgcggccg gcgatgcgca ggtgcgcgac gaagccgtcg
     1621 tgccagtcgc gcttgtcgcc gtcgatgaag aacgcgagcg gcgcgcgcgg cggcagcgcg
     1681 gcgccgagcg tgcggtacag cgtcgcgcaa gcggccgtac ggtccagcag gcagagcaaa
     1741 tcgcggggat cggaccccgc gaccggggtc gtcgccgcgg gatcgagcgc cggccacacc
     1801 gcgcgcagca cccgcgccgg gatcatctcg gcggcgacgc cgccggcctc ggtcgcgacg
     1861 aggccggcaa agcgccgctc gcggtccagc cagacgccgc cgccgctgaa gccggcagtg
     1921 atccggatcg ctgcgttcac ggtgttgcgg gcgctgctgt cgagcagcgt gcggccgttg
     1981 gtgcgcgggc cgagccgcgt gcagcgggtc gggatgccgg catcgctgcc gtccggcagt
     2041 ccccaggtcc aggcgtcgcc gtcgccggtc caccgccagc cgtcggccgt gaagtccgca
     2101 cacggcacgc ccggtgccgg tgcgtcgagg cgcagcacgg cgatgtcgac ccgtccgctg
     2161 ggttcctcgg cttcgtccgg ccggcggttg cgctgccagc cgccatcggc gacgcacgcc
     2221 gtgtagccgg cggccggcgc ggggccgaac ggaaagttca cgcggaccga tccggcgggc
     2281 ggctcgtcgc gaaactgcgc gtcgggacag cccagcgcat cggccacgac gtgcgcgcag
     2341 ctcagcacgt ggccgccggg ccgcacgtgc aggcccacgc cgacgtagcg cccgccggcc
     2401 gtggcgatgc gagcgaccgg tccgagatcc ctttcgtcca caggggcgtc cctggcgtgg
     2461 attcagcgct tgccgtagcg cttgcgcggc accggttcgg cccgcttcca gttcagcttc
     2521 accgcgaagt tcgcctcgct gccggccttg gcgatgaccg cgccggcagc agcactcaac
     2581 ttgatcccga actgcacctc gacctgatcg ggtcgagccg tgagcccgct cagcgtatca
     2641 agcacggcat cggcaagtgg ctgcagccgg gcgagggctt cctgcacgct gcgcggcagc
     2701 tcgccggggt ccggcgcgcg gctgaccggc cggaagctca ggccgaccag atcatcttcg
     2761 acctcggccg gcacgctttc gccggaagcc agcgtgaaca ccacaatttt cgtgtcgcca
     2821 gtgcgcattg tcggaacctc cccgggcggc ctgccgcggt gagtcaggca cgcgtgcagc
     2881 ggtttcgcaa ggcgtggacg gccgggtgtc gagtcggcaa gggttcagcg actgagcgac
     2941 cccgagcgcg tgcgcgtcgc tccgcgcggg ccggtcgtcg tgctgctgcc gctgcagctg
     3001 ccatcggcgc agcccgcctc gccctgatgc gtggacgtcc gcccggccgg gccggtgcgc
     3061 gtgacgctgc gcgagcagct gcggtccgcg cagctgccgg aggcatcgac gctgctgctg
     3121 ccgtgcgggc cgctgctgct gccgtggcgg gtccacgcga cggcgtcggc gagcggcacg
     3181 ccggcgagca gggtggtgag gacgaggctg acgagggcgg tgcgcatgac gggctcccgg
     3241 gatgcaggac agggaaggga caggccgctc ccatcctgcc ctgacggtgt gacggctgcg
     3301 tcgtgcgcag gtcacgactg gtatcgggct gccggtgccg cttcgaccgg acacctccgg
     3361 ctgccgcccg gccggtgcga cccggcgact acgctttccg catcccggcg gccggttttc
     3421 acggcgggtt gcccgggcag ctcgtggtca acaaaaaaac catgcgaact catgactttg
     3481 ggccagttct ggtacaatga tggcgctggt aaaatggtct taccacttag gagagaagat
     3541 gaacgatacc actggcgccg cccgcagcgc cccgccgcgc gtcggcctgt tcgtgacctg
     3601 tctggccgat acgatccggc cgtcggtcgc gtttgcgacc gtcaagctgc tcgaagacgc
     3661 cggctgccgg gtcgaggcac cggcgagcca gacctgctgc ggccagccgg ccttcaactc
     3721 cggggatcgc gccgatgccc gcgccctcgc cgagcagacc atcgccgcgt tcgagggctt
     3781 cgactacgtc gtcgggccgt cgggctcgtg cctcggcatg ctgcgccact atccggagct
     3841 gttcgagacc ggcagcccct ggcacgcgcg cgccgaaagg ctggccgcga agtgcttcga
     3901 gatcacgtcc ttcctcgtcg acgtgctcgg cgtcgagcgc gtgcaggcgc gttacgacgg
     3961 caccgtcacc taccacgatt cctgctccgg cctgcgcgaa ctcggcgtca aggcgcagcc
     4021 gcgcaagctg ctcgccacgg tcgagggcct gacgctcacc gagatgaacg agaacgaggt
     4081 ctgctgcggc ttcggcggca ccttctgcat caagtatccg gacatctcca accgcatggt
     4141 caccgagaag gtcgccaaca tccgcgccag cggcgccggc acgctgctcg ccggcgatct
     4201 cggctgcctg atgaacatgg ccggaaagct caagcgccag ggctcggacg tgcgtgtctg
     4261 gcacgtcgcc gaagtgctgg ccggcatgac cgacggcgcg cccattgcgg acgccgcccc
     4321 gagccgctga agccccggag cccgagccat gcagatcacc agtccccgtt tcaagcagaa
     4381 cgcccacgag gcactcggcg atgccgcgct gcagcgtgcc ctcggcaacc tcaaggccgg
     4441 ctttccgggc aagcgcgccg ccgcggtcgc gcgcatgccc gagttcgagc agctgcgcga
     4501 tgccgcgcgc gatctgaaga accacatcct cgaacacctc gacttctacc tcgaaacctt
     4561 cgaggccaac gtcaagcgcc gcggcggtca cgtgcactgg gcgcgcgatg ccgaggaagc
     4621 gcgcgcgatc gtgctcgaca tctgccgcaa ggccggggcg aagacggtca ccaagggcaa
     4681 gtcgatgatc accgaggaga tcggcctcaa cgatcacctc gcggccaacg gcatcgagcc
     4741 gatcgagacc gatctcggcg agtacatcat ccagctgcgc ggcgagcacc cgagccacat
     4801 catcgcgccg gcgatccacg tcaacctcga ccaggtttcc gacgccttcc acgcccatca
     4861 cggcaagtac ggcaagaccg agcgccagac cgagccgcgg gcgctgatga acgaggcgcg
     4921 cgagatcctg cgcgacaagt tcatcgccgc cgacgtcggc atcaccgggg cgaacttctg
     4981 catcgccgag accggcacga cggtcatcgt caccaacgag ggcaacggcg acctgacgca
     5041 gacgctgccg cgcgtgcacg tggccgtcac cagcatcgag aaggtcgtgc cgacgctgga
     5101 agacgccacg ctgctgcacc ggctgctgtc gcgctcggcg accgggcagg agatcgaggt
     5161 ctacggcacc tggtcctccg gcccgcgccg gcccgaagac ccggacggcc ctgccgagta
     5221 ccacgtcgtg ctgctcgaca acggccgctc gaacatgctc ggcaacgagt tccgcgagat
     5281 gctgcgctgc atccgctgct cggcgtgcat gaaccactgc ccggtgtacg gcgcgatcgg
     5341 cggccacgcc tacggctggg tgtatccggg gccgatgggt tcggtgctga caccagcctt
     5401 catcggcgtc gaacaggccg gcaacctgcc gaacgccagc accttctgcg ggcgctgcga
     5461 gagcgtctgc ccggtgcgca tcccgctgcc gaagctgatg cgtcactggc gcgcgcgcga
     5521 gttcgaaaag cacctgacgc cgcagggctt ccgcttcggg ctcggcgtct gggcctggct
     5581 cgcgcgccgg ccgaagctgt accggctggc cacccaggtg gcgacgcgcg tgctgaagcc
     5641 gctcggcggc aaaaccaacc gcatcggctc gctgccgctc gccggcggct ggacggcgtg
     5701 gcgcgacctg ccggcgccgc agggcaggac cttcttcgaa cagtggaaag accggcagca
     5761 ggccgggggt gcgaaatgag cgggcgtgag gcgatcctcg gcaacatccg caagggcctc
     5821 aagcgcagcg gcccgctggc gggcacggcg ctggagcttt gtcagacgcg gctgcgcgag
     5881 caccggccga acctgatccc ggcgcgcgcg cagctgccgc acgccggcca ggtcgagctg
     5941 ttcgtgcaga tggcgcagga ggcgtcggca acggtcgcgc gcgtcacgga gcgcgccgcc
     6001 gtgccggccg aggtcgcgcg ctatctggcc gacaccggct tcgccgccga ggtcatcgtc
     6061 gcgccggatg cgtcgatcgc cggcctcgac tgggccgccg ccggtgtggc caccgaagcc
     6121 cgccacgccc gcaacggcga tcgcgccacc gtcgcaccgg ccttcgccgg catcgccgag
     6181 accggcacgc tgatgctgct cgccggcccg cagagcccga cgacgctgaa cttcctgccc
     6241 gatgcgcaca tcgtcgtgct cgatgccgcg cgcatcgtcg gcccctacga ggacgcctgg
     6301 gcgatgctgc gcagcgagcg cggcgagggc tggatgccgc gcgtcgtcaa ctggatcacc
     6361 ggcccgtccc gctcggccga catcgagcag aagctgcaga tgggcgcaca cggcccgatc
     6421 cggctgcaca tcgtgctgat cggcgactga gcgggagctg ccgtgaacga cgcgggcgct
     6481 gtccgggagg gcggcgcccg tgaagtttcc ggggacaggg ttggccgtga aacgcttacg
     6541 gcaggtcgag cagatctttc aggaatgtgc gcagtgcagc gatgcgcggc tgcgcgagcg
     6601 cgctgcctgt cagccagcga tcctggcgca gcgtgacagc gatcgatgca cccggctgca
     6661 gcaatgcgcc gatcaccgca atcgtggctt tctgccggtt ggcgcctttt tccagattgc
     6721 ggcgttcgga ttcgtcgagg tgaccagcgc catccgtcat gtaattgcag gccagtacct
     6781 gcagttgcgg atagtgtgtg gcggcgcaat cttccagcag cgtttccagc gtgccggggc
     6841 cgagattatc aggaaagacc atgatgccag tgcgcggtgg accggcatgg acgacgccgg
     6901 gtgtgctggc cgcgaaagtg agattgcagc cagccagtgc tgcgcacagt ttcgacatgc
     6961 gcgcactcgc ggtttgctga tccgcatcgc agacgatgcc gatggcctgc aattgatcct
     7021 gtttactgcc gagtcctgac agtgtggctt cgagggcgga agcaatgttc gtgtcgctgt
     7081 cggcagtcct gatcgcgatc gaatgcgtgt tgttttcgac aaatatcggt acatccatgc
     7141 gtttcagcag atcgccgccc tgcgggaatt tcaggtttgc gtacagggga cgccagtagg
     7201 aagcaagctt tttttcattc tgaatggttt tcaggttgaa gtcggataga agtttggcaa
     7261 tgacggccgc atcgtgttgc ccctcgacga tcagcaggcc gtattgcatc agcgcacatc
     7321 catgccaaag tcgtagcgca tttcgcgcag cagctccccg cgaatgcggg tggcgacggt
     7381 gccgttgtca gtgtgttcca gtcgatgcac gacgatgtcg cccggatgat cggtttcggc
     7441 agccatcagg gcgtcgacgg cttcgaggct gtgggtggtg cagaaaagct gaattttgct
     7501 ttgtcggctg actttgatca gccagccaaa gaacgtggtc aatgcggttg gatgtatggc
     7561 ggtttcaatc tcgtcaagca gcaataaacc atggcgtgcc gaatgcaacg acaacgcaat
     7621 agaaaaggct cgccgtaagc catcaccaaa aatgctcagc ggtgccggcg atttcgggcc
     7681 aagtcgtcgg tgtcgcaggt aggggcgtga attctttcca tcaccatccg tcagcagcag
     7741 aaaatcaatg atgtcctggt caaatagctg caatgcatcc aggactggct ttttgtcgtt
     7801 tttctgcaag acactggaga tttcgactgt ctgaaatacc gtgagccggt gggcgactga
     7861 agataacaaa cggagcggga gcatcttggg ttccgggtcg ccccggtcca ctgaccagtt
     7921 gctgccccag aaaccaagcg tcgcattctc ggcattgttt tcatcgagaa accggctcac
     7981 atcgacgtat cgtctcggca ttaccagtcc tgccacatcc ggatcggtcg cgttgaccag
     8041 gcggatggag gcttcgacgc gggatgcttc tttttttccg ctggcgaatc cggacaggcg
     8101 tacaggcgag tgttcatctc gctctgcaga atgcggaaaa aaccagttca cggcatcaga
     8161 caaaaggaag ccagggccgc caatttcgcg caggcttgcg ctggccagcc aggttttgtc
     8221 atcgaaaggc cgtgcgaaca gcgccagcgc ctccagcacc gaggtcttgc cggaattgtt
     8281 gtccccgacc agcacgttga cgctgccgag tccttccagc ttcacgtcgc gcagaccgcg
     8341 gaaggcgccg atggtcaggc tgtccagatg tcgcatcgcc gtcctccgcg tcgtatcagc
     8401 cgcttattcc gccagccact tccttgcccg ctgcaccgcc gcccgcacct gcgccggcgc
     8461 cgtg
//
LOCUS       DAOQIE010000165         8383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4328_length_8383_cov_7.509486, whole genome shotgun sequence.
ACCESSION   DAOQIE010000165 DAOQIE010000000
VERSION     DAOQIE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4328_length_8383_cov_7.509486"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PK144_11010"
     CDS             <1..1545
                     /locus_tag="PK144_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019333145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNF66302.1"
                     /translation="FAHRYGLPVEPVIRTPAGDATPLPWQPAYAEHGTLIHSGEFDGL
                     DFDGAYAAMAERLGALGLGGPQTRFRLRDWGISRQRYWGCPIPIVHCADCGDVPVPAD
                     QLPVVLPEDVVPDGSGNPLAKLPAFYETSCPKCGAPARRETDTMDTFVESSWYYARYA
                     SPKFDGGMVEPGAAHYWLPVDQYVGGIEHAILHLLYARFFHKLMRDEGLAGSDEPFVR
                     LLTQGMVVAETYFREDAAGRKSWFNPAEVRVERDGKGRIVSAILPADGQPVQFGGIEK
                     MSKSKNNGIDPQSLIDRCGADTARLFMMFAAPPDASLEWSDAGIEGASRFLKRLWAWG
                     HEHAAALAARPAIDAGALDAGLKALRREIHEALGQALFDYQRQQFNTVVSAAMKMLNA
                     LAKLPEGAAADGVRAEGWAILLRVLAPVVPHVTHVLWAALDRGPLLDAPLPQPDAGAL
                     ARDSLTLAVQVNGKLRAQIEVAADADRLTIETAALADAQVQRFLEGRPAKKVIVVPGK
                     LVNVVG"
     gene            1548..2075
                     /gene="lptE"
                     /locus_tag="PK144_11015"
     CDS             1548..2075
                     /gene="lptE"
                     /locus_tag="PK144_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNF66303.1"
                     /translation="MASVLRIAILKAVFVVLLAGLSGCGFQLRGQLALPPVLERTHLQ
                     VAAGERSTLYQHLKAGLQVNGVSLVSDRRSATAVVEVGSESVSKQTVVKSGSGSALEY
                     ELFYSVDFRVTGADNKVLISQQNVRLSRDLLYDNATVIGRDTGETRATEDMRKDAAQA
                     ILRRIAALSSAQPAR"
     gene            2090..3118
                     /gene="holA"
                     /locus_tag="PK144_11020"
     CDS             2090..3118
                     /gene="holA"
                     /locus_tag="PK144_11020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNF66304.1"
                     /translation="MRQRPEQLAATLARGLKPVYLIHGEEPLQALEAADAVRAAARAQ
                     GFSEREVLNADAGFDWNSLAAAAGTQSLFGERRLVELRIPSGKPGDAGARALRAWCSR
                     PPEDTVLMVASGRLDAQQMKSAWFTALDEAGVSVQTFALETRAFPEWLTRRALARGLK
                     LAPEAVALLATQTEGNLLAAAQELDKLWMLHGDAPLDAEALLAAVADSARFGIYDLAD
                     AALDGDPARCARILGSLRAEGEEAVLTLWALAREIRTLLQAAQECARGSAPEAALTRL
                     KVWDKRKPLMRRALARLDAARLQRLLADCARVDRTVKGQEAGEPWDALLALALGLAGC
                     DAGLARSA"
     gene            3209..4498
                     /locus_tag="PK144_11025"
     CDS             3209..4498
                     /locus_tag="PK144_11025"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNF66305.1"
                     /translation="MTASVQETQAGADVRSYMQELGRRARVAARAMSRADTAAKNAAL
                     LAIAAQIEAALPALLEANAADLEAARAGGLGAALLDRLALGEKGIRGMAQGLREVAVQ
                     PDPVGEITDLKFRPSGIQVGRMRVPLGVIGIIYESRPNVTADAAALCLKAGNACILRG
                     GSEAARSNRAIAACIAAGLEAAGLPAEAVQVVATTDRAAVGELIRMEQYVDVIVPRGG
                     KSLIARIAAEARVPVIKHLDGICHVYVDDRADIGKAVRVAFNAKCRRYGICGAMETLL
                     VHEAVAPYVLPQLAELYGAAGVELRGCERTRQLLPAALAATEEDWSTEYLDAILSIRV
                     VDGLDQAIAHIEQYGSHHTDSIITEDYTRARRFLREVDSASVMVNASTQFADGGEYGL
                     GAEIGISTDKLHARGPVGVEGLTTQKYVVLGDGSTRA"
     gene            4491..5159
                     /locus_tag="PK144_11030"
     CDS             4491..5159
                     /locus_tag="PK144_11030"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNF66306.1"
                     /translation="MPESHAALALDDDERAELDAVLVAGGHDLEYADGLFCALICGPA
                     AVDRTCWLTLLFPVPVTGDAAARVLELVERHWNDIAHRLSLTREALDEDSFYLPFTHV
                     RSGAPGEALTGQRWAEGFRAGMAMAPDAWRPLLADEQVLAQLAPIVSLARPAGDARFG
                     AAADREALTDLLPLSAYRLARQLAVRRRTPLRVERPGRNAPCPCGSGRKYKHCCGAGR
                     PVTH"
     gene            5177..5833
                     /gene="nadD"
                     /locus_tag="PK144_11035"
     CDS             5177..5833
                     /gene="nadD"
                     /locus_tag="PK144_11035"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNF66307.1"
                     /translation="MQPAIGLYGGTFDPVHHAHLRLALEVREALGLAGLRFVPCHVPA
                     LRAAPGASGEQRRAMLELAIRGEPGLSVDDRELRRSGPSYTIDTLASLRAELGPEVPL
                     VWLLGADAWAQLPRWRDWQRLTDFAHLAVLARPGAAACETPADPALAAFLAEHRADPG
                     CLRERAAGGVWFQPASLLDISASAVRAALRAGRSARYWLPEAVLDYIARHGLYRPAGA
                     "
     gene            5879..6199
                     /gene="rsfS"
                     /locus_tag="PK144_11040"
     CDS             5879..6199
                     /gene="rsfS"
                     /locus_tag="PK144_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNF66308.1"
                     /translation="MSAEELKNVVVDALDEVKGVDIRVLDVREKSSFADLMVIATGTS
                     DRHVKALAGKVQEKCREAGERPIGVEGEREGQWVLIDLADVVVHVMTAQTRDFYNLEK
                     LWGA"
     gene            6220..6690
                     /gene="rlmH"
                     /locus_tag="PK144_11045"
     CDS             6220..6690
                     /gene="rlmH"
                     /locus_tag="PK144_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNF66309.1"
                     /translation="MRIHLLAVGQKMPAWVEAGYVEYAGRLPRECELRLVEIAAGRRT
                     KNADVARILKDEGERTLAAVPKGAHVVALDVRGREHTTETLAQRLAAWLADGRDVALL
                     VGGPEGLDAACTAAAGETWSLSRLTFPHPLVRIVVAESLYRAWSLLNHHPYHRA"
     gene            6814..7389
                     /locus_tag="PK144_11050"
     CDS             6814..7389
                     /locus_tag="PK144_11050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNF66310.1"
                     /translation="MSTVLRLASTSPRRRELLEQVGIGYTLIRAEVDETPLPAEPPAA
                     YVERLARAKAEAGLQAAGGEGLVLGADTTVVADGLILGKPRDRAEGLALLERLSGREH
                     AVLSAVALATAGRTEARVQVSRVHFRATTAAEREAYWATGEPADKAGGYAVQGRAAVF
                     IERLDGSYSGVMGLPLYETCELLRAFGVEVL"
     gene            7470..>8383
                     /locus_tag="PK144_11055"
     CDS             7470..>8383
                     /locus_tag="PK144_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNF66311.1"
                     /translation="MCEEILMNVTPRETRVAIVENGVLQEIYIERTRRRGLVGNVYKG
                     RVVRVLPGMEAAFVDIGLERAAFLHVSDIQTCETLTGEHGPGERRPPEITDLVREGQE
                     LLVQVVKDPLGTKGARLTTHVTIPSRYLVYLPGSDAVGVSVKIEDETERARLKGIISE
                     YRAEFGGGYIARTAGEGATAPALRADMQFLQRLWTSIRERAREARPGTVVHEDLPLTL
                     RVLRDFVGGKVDKVRVDSNETYERMEQFAGRYVPEMLPKLEHYPGERPIFDLYGIEDE
                     LARALDKRVQLKSGGYLIIDQTEAMTTI"
BASE COUNT         1172 a         3066 c         3021 g         1124 t
ORIGIN      
        1 ttcgcgcacc gctacggcct gccggtcgag ccggtgatcc gcacgccggc cggcgatgcg
       61 acgccgctgc cctggcagcc ggcctacgcc gagcacggca cgctgatcca ttccggcgag
      121 ttcgacggcc tcgacttcga cggcgcctat gcggcgatgg ccgagcgcct cggcgcgctc
      181 ggtctcggcg ggccgcagac ccgcttccgg ctgcgcgact ggggcatctc gcgccagcgc
      241 tactggggct gcccgatccc gatcgtgcac tgcgccgact gcggcgacgt gccggtgccg
      301 gccgatcagc tgccggtcgt gctgcccgag gacgtggtcc cggacggcag cggcaacccg
      361 ctcgcgaagc tgccggcctt ctacgagacc tcctgcccga agtgcggcgc gccggcccgg
      421 cgcgagaccg acacgatgga caccttcgtc gagtcgagct ggtactacgc gcgctacgcc
      481 tcgccgaagt tcgacggcgg catggtcgag cccggggccg cgcactactg gctgccggtc
      541 gatcagtacg tcggcggcat cgagcacgcg atcctgcacc tgctgtacgc gcgcttcttc
      601 cacaagctga tgcgcgacga gggtctggcc ggctccgacg agcccttcgt gcgcctgctg
      661 acccagggca tggtcgtcgc cgagacctac ttccgcgagg acgccgccgg ccgcaagagc
      721 tggttcaacc cggccgaggt ccgggtcgag cgcgacggca agggccgcat cgtctcggcg
      781 atcctgcccg ccgacggcca gccggtgcag ttcggcggca tcgagaagat gtcgaagtcc
      841 aagaacaacg gcatcgatcc gcagtcgctg atcgatcgct gcggtgccga caccgcgcgc
      901 ctgttcatga tgttcgccgc ccccccggat gcctcgctgg agtggtccga cgccggcatc
      961 gagggcgcct cgcgcttcct caagcgcctg tgggcctggg ggcacgagca tgccgcggcg
     1021 ctcgccgccc gcccggccat cgacgccggc gcgctcgacg ccggcctcaa ggccctgcgc
     1081 cgcgagattc acgaagccct cggtcaggca ctgttcgact accagcgcca gcagttcaac
     1141 accgtcgtct cggcggcgat gaagatgctg aacgcgctcg cgaagctgcc ggagggcgct
     1201 gcggccgacg gcgtgcgggc cgaaggctgg gcgatcctgc tgcgcgtgct ggcaccggtc
     1261 gtgccgcatg tcacgcacgt gctgtgggcc gcgctcgatc gcggtccgct gctcgatgcg
     1321 ccgctgccgc agcccgatgc cggcgcgctc gcgcgtgaca gcctgacgct ggccgtgcag
     1381 gtcaacggca agctgcgcgc gcagatcgag gtggcggccg atgccgatcg cctgacgatc
     1441 gagaccgccg cgctcgccga tgcccaggtg cagcgcttcc tggagggccg tccggcgaag
     1501 aaggtcatcg tcgtgcccgg caagctcgtc aacgtggtcg gctgaacatg gcaagcgtgc
     1561 tgcgcatcgc gatcctgaag gcggtcttcg tcgtgctgct ggccgggctg agcggctgcg
     1621 gcttccagct gcgcggccag ctcgcgctgc cgccggtgct cgaacgcacg catctgcagg
     1681 tggcggccgg cgagcgctcg acgctgtacc agcacctgaa ggccgggctg caggtcaacg
     1741 gcgtcagcct cgtcagcgat cgccgctcgg cgacggccgt ggtcgaggtc ggcagcgagt
     1801 cggtcagcaa gcagacggtg gtcaagagcg gctcgggcag cgcgcttgaa tacgaactgt
     1861 tctacagcgt cgatttccgc gtcaccggtg ccgataacaa ggtgctgatc agccagcaga
     1921 acgtacgcct gagccgcgac ctgctgtacg acaacgccac ggtgatcggc cgcgataccg
     1981 gcgagacgcg tgcgaccgag gacatgcgca aggatgccgc gcaggcgatc ctgcggcgca
     2041 ttgcggcgct ttcgtcggcg cagccggcac gctgagcacg ggagcgggca tgcgccagcg
     2101 tcccgagcag ctcgccgcga cgctcgcgcg cggactgaag ccggtgtacc tgatccacgg
     2161 cgaggagccg ctgcaggcgc tcgaggccgc cgacgccgtg cgcgccgcgg cgcgtgcgca
     2221 gggcttcagc gagcgcgagg tgctgaatgc cgatgccggc ttcgactgga acagcctcgc
     2281 ggcggcggcc ggcacgcagt cgctgttcgg cgagcgccgg ctggtggagc tgcgcatccc
     2341 cagcggcaag cccggcgacg ccggcgcccg ggcgctgcgc gcgtggtgca gccgcccgcc
     2401 cgaggatacg gtgctgatgg tggccagtgg caggctggac gcgcagcaga tgaagtcggc
     2461 ctggttcacg gcactcgacg aggccggcgt cagcgtgcag accttcgcgc tcgagacgcg
     2521 ggcgtttccg gagtggctga cgcgccgggc cctggcgcgc ggcctgaagc tcgcaccgga
     2581 agccgtggcg ctgctggcga cgcagaccga gggcaacctg ctcgccgccg cgcaggagct
     2641 cgacaagctg tggatgctgc acggcgacgc cccgctcgat gccgaggcgc tgctggccgc
     2701 ggtcgccgac agcgcgcgct tcggcatcta cgacctcgcc gatgccgcgc tcgatggcga
     2761 tccggcgcgc tgcgcgcgca tcctcggcag cctgcgcgcc gagggcgagg aagccgtgct
     2821 gacgctctgg gcgctggcgc gcgagatccg cacgctgctg caggccgcgc aggagtgcgc
     2881 acgcggcagc gcgcccgagg cggcgctgac gcggctgaag gtctgggaca agcgcaagcc
     2941 gctgatgcgc cgggcgctgg cgcgactcga tgcggcacgg ctgcagcggc tgctcgccga
     3001 ctgcgcgcgc gtcgatcgca cggtcaaggg ccaggaggcc ggcgagccct gggatgcgct
     3061 gctcgcgctc gcgctcgggc tggccggctg cgacgccggg ctggcgcgca gcgcctgagc
     3121 ccccatatcc cggcagaccc ggccgctttc cggccggacg gaatgttcag gcagtcgccc
     3181 gcagcggctg cgcttcgagg tgaagacgat gactgcaagc gtgcaggaaa cccaggccgg
     3241 cgccgacgtg cgcagctaca tgcaggaact cggccggcgc gcgcgcgtcg cggcgcgggc
     3301 gatgagccgt gccgacaccg ccgcgaagaa cgccgcgctg ctcgcgatcg ccgcgcagat
     3361 cgaggccgcg ctgccggcgc tgctcgaagc caacgccgcc gatctcgagg ccgcgcgcgc
     3421 cggcggcctc ggcgcggcgc tgctcgatcg gctcgcgctc ggcgagaagg gcatccgcgg
     3481 catggcccag ggcctgcgcg aggtcgccgt ccagcccgat ccggtcggcg agatcaccga
     3541 cctgaagttc cgcccctcgg gcatccaggt cgggcgcatg cgcgtgccgc tcggcgtcat
     3601 cggcatcatt tatgaatcgc gtccgaacgt gaccgccgat gccgcggcgc tgtgcctgaa
     3661 ggccggcaac gcctgcatcc tgcgcggcgg ctccgaggcg gcgcgctcga accgcgcgat
     3721 cgccgcctgc atcgccgccg gcctcgaagc cgccggcctg ccggccgagg ccgtgcaggt
     3781 ggtcgcgacc accgaccgcg ccgcggtcgg cgagctgatc cgcatggagc agtacgtcga
     3841 tgtcatcgtg ccgcgcggcg gcaagagcct gatcgcgcgc atcgccgccg aggcgcgcgt
     3901 gccggtgatc aagcacctcg acggcatctg ccacgtctac gtcgacgatc gcgccgacat
     3961 cggcaaggcc gtgcgcgtcg ccttcaacgc caagtgccgg cgctacggca tctgcggcgc
     4021 gatggaaacc ctgctcgtgc acgaggcggt cgcgccgtac gtgctgccgc agctcgccga
     4081 gctctacggc gccgccggcg tcgagctgcg cggctgcgag cgcacgcgcc agctgctgcc
     4141 ggccgcgctc gcggcgaccg aggaggactg gtcgaccgaa tacctcgacg cgatcctgtc
     4201 gatccgcgtc gtcgacggcc tcgaccaggc gatcgcgcac atcgagcagt acggctcgca
     4261 ccacaccgac agcatcatca ccgaggacta cacgcgcgcc cggcgcttcc tgcgcgaggt
     4321 cgattcggcc tcggtcatgg tcaacgcctc gacgcagttc gccgatggcg gcgagtacgg
     4381 gctcggtgcc gagatcggca tctcgaccga caagctgcac gcccgcggcc ccgtgggcgt
     4441 cgagggcctg accacgcaga agtacgtcgt gctcggggat ggcagcaccc gtgcctgagt
     4501 cgcacgccgc gctggcgctc gatgacgacg agcgcgccga actcgacgcg gtgctcgtcg
     4561 ccggcgggca cgatctcgaa tacgccgacg gcctgttctg cgcgctgatc tgcggtccgg
     4621 ccgcggtcga tcgcacctgc tggctgacgc tgctgttccc ggtgccggtc accggggacg
     4681 ccgccgcgcg cgtgctggaa ctcgtcgagc gccactggaa cgacatcgcg caccggctgt
     4741 cgctcacgcg cgaggcgctc gacgaggaca gcttctatct gccgttcacg cacgtgcgca
     4801 gcggcgcgcc aggcgaggcg ctgaccggcc agcgctgggc cgagggcttt cgcgccggca
     4861 tggcgatggc gccggatgcc tggcggccgc tgctcgccga cgagcaggtg ctcgcgcagc
     4921 tcgcgccgat cgtgtcgctg gcgcgcccgg ccggcgatgc gcgtttcggg gcggcggccg
     4981 atcgcgaggc gctgaccgac ctgctgccgc tctcggccta ccggctcgcg cgccagctcg
     5041 ccgtccgccg gcgcacgccg ctgcgcgtcg agcggcccgg gcgcaacgcg ccctgcccgt
     5101 gcggcagcgg gcgcaagtac aagcactgct gcggggccgg ccggcccgtg acgcactgaa
     5161 ggccccctgc gtgcccatgc agcccgcgat cggcctctac ggcggcacct tcgacccggt
     5221 ccaccacgcc cacctgcgcc tcgcgctgga ggtgcgcgag gcgctcgggc tcgccgggct
     5281 gcgcttcgtg ccctgccatg tgccggcctt gcgcgccgcc cccggcgcga gcggcgagca
     5341 gcgccgggcg atgctggaac tcgcgatccg cggcgaaccg ggcctgagtg tcgatgatcg
     5401 cgagctgcgc cgcagcgggc cgtcgtacac gatcgacacg ctcgcgagcc tgcgcgccga
     5461 actcggtccc gaggtgccgc tggtctggct gctcggggcc gatgcctggg cgcagctgcc
     5521 gcgctggcgc gactggcagc ggctgaccga ctttgcgcat ctggccgtgc tggcacggcc
     5581 gggtgctgcg gcttgcgaga ccccggccga tcctgcgctc gcggcctttc tggccgaaca
     5641 ccgcgccgat cccggctgcc tgcgcgagcg cgccgccggc ggcgtgtggt tccagccggc
     5701 atctttgctc gacatctccg cgagcgcggt gcgtgccgcg ctcagggccg ggcgctcggc
     5761 acgctactgg ctgcccgaag ccgtgctcga ctacatcgcc cgccacggcc tgtaccggcc
     5821 ggccggcgcc tgatccatcc ccccactgcc gcgaatacgg gaaaccaccg acaacatgct
     5881 gtccgccgaa gaactgaaga acgtcgtcgt cgatgccctc gacgaggtca agggcgtcga
     5941 catccgcgtg ctcgacgtgc gcgagaaatc gagcttcgcc gacctgatgg tcatcgccac
     6001 cggcacctcc gaccgccacg tcaaggcgct cgccggcaag gtccaggaga aatgccgcga
     6061 ggccggcgaa cgcccgatcg gcgtcgaggg cgagcgcgag ggccagtggg tgctgatcga
     6121 cctcgccgac gtggtcgtgc acgtgatgac cgcgcagacc cgcgacttct acaacctcga
     6181 aaagctctgg ggcgcctgag ccgcgcagcg ggcgcaccca tgcgcatcca cctgctcgcc
     6241 gtcggccaga agatgccggc ctgggtcgag gccggctacg tcgagtacgc cggccggctg
     6301 ccgcgcgaat gcgagctgcg cctggtcgag atcgccgccg gccggcgcac gaagaacgcc
     6361 gatgtcgcgc gcatcctgaa ggacgagggc gagcgcacgc tggcggcggt gccgaagggc
     6421 gcgcacgtcg tcgcgctcga cgtgcgcggg cgcgagcaca cgaccgaaac cctcgcgcag
     6481 cgcctcgccg cctggctcgc cgacggtcgc gacgtggcgc tgctggtcgg cggccccgag
     6541 gggctcgatg ccgcctgcac ggctgcggcc ggcgagacct ggtcgctgtc gcggctgacc
     6601 ttcccgcatc cgctggtgcg catcgtcgtc gccgagagcc tgtaccgggc ctggagcctg
     6661 ctgaaccacc atccgtacca tcgcgcctga gccgcgccgc aagccgcgcc tgatccggag
     6721 ccgaccggca aagcccccgc agggcagggt ccgggaaagg cgcgccccgg cccggtcccg
     6781 acgaacccgc atccgccccc gcccggagtc ccgatgtcca ccgtcctgcg cctcgcctcg
     6841 acctccccgc gccgccgcga actgcttgag caggtcggca tcggctacac gctgatccgc
     6901 gccgaggtcg atgaaacccc gctgcccgcg gagccgccgg cggcctacgt cgagcgcctc
     6961 gcgcgcgcca aggccgaggc gggcctgcag gcggccggcg gcgaggggct ggtgctcggg
     7021 gccgacacca ccgtcgttgc cgacgggctg atcctcggca agccgcgcga ccgcgccgag
     7081 ggcctcgcgc tgctcgaacg cctgtccggg cgcgaacacg cggtattgtc ggcggtcgca
     7141 ctggcgacgg ccggacgcac cgaggcgcgc gtgcaggtgt cgcgcgtgca tttccgggcg
     7201 accacggccg ccgagcgcga agcctactgg gccaccggcg agcccgccga caaggccggc
     7261 ggctacgctg tgcagggcag ggcggcggtg ttcatcgaac ggctcgacgg cagctactcg
     7321 ggcgtcatgg ggctgccgct ttacgagacc tgcgaattgc tgcgcgcctt cggcgtggag
     7381 gttctgtgag cgaaggcatc gacgagagca gcggcaccgg catgcccggc gcgacgccgg
     7441 ccccggccgg cgtcaccgag accggacgca tctgcgagga aatcctgatg aacgtcacgc
     7501 cgcgcgagac gcgcgtggcg atcgtcgaga acggcgtgct gcaggagatc tacatcgagc
     7561 gcacgcgccg ccgcggtctg gtcggcaacg tctacaaggg ccgcgtcgtg cgcgtgctgc
     7621 cgggcatgga ggcggccttc gtcgacatcg gcctggagcg cgccgccttc ctgcacgtct
     7681 ccgacatcca gacctgcgag acgctgaccg gcgagcacgg ccccggcgag cgtcgtccgc
     7741 ccgagatcac cgacctcgtg cgcgaaggtc aggaactgct cgtgcaggtg gtcaaggacc
     7801 cgctcggcac caagggcgcg cgcctgacca cgcacgtgac catcccgtcg cgctacctcg
     7861 tctacctgcc cggctccgat gccgtcggcg tgtcggtgaa gatcgaggac gagaccgagc
     7921 gcgcgcgcct caagggcatc atcagcgagt accgcgccga gttcggcggc ggctacatcg
     7981 cgcgcacggc cggcgagggc gcgaccgcgc cggcgctgcg cgccgacatg cagttcctgc
     8041 agcggctgtg gacctcgatc cgcgagcgcg cgcgcgaggc ccggcccggc accgtcgtgc
     8101 acgaggacct gccgctgacg ctgcgcgtgc tgcgcgattt cgtcggcggc aaggtcgaca
     8161 aggtgcgcgt cgattccaac gagacctacg agcgcatgga gcaattcgcc ggccgctacg
     8221 tgccggagat gctgccgaag ctggagcact accccggcga gcggccgatc ttcgatcttt
     8281 acggcatcga ggacgaactc gcgcgcgcgc tcgacaagcg cgtgcagctg aagtccggcg
     8341 gctacctgat catcgaccag accgaggcga tgacgacgat cga
//
LOCUS       DAOQIE010000166         8373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4333_length_8373_cov_8.135850, whole genome shotgun sequence.
ACCESSION   DAOQIE010000166 DAOQIE010000000
VERSION     DAOQIE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4333_length_8373_cov_8.135850"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PK144_11060"
     CDS             complement(<1..1099)
                     /locus_tag="PK144_11060"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HNF66312.1"
                     /translation="MARISRQAYADMYGPTTGDRVRLGDTGLLIEIEADYTVYGDEVK
                     FGGGKVIRDGMGQSQLTRAQGAVDTVITNAVIVDHWGIVKADIGLKDGRIAAIGKAGN
                     PDVQPGVSIVIGPGTEVIAGEGLIVTAGGLDTHIHFICPQQIEEALMSGVTTMIGGGT
                     GPATGTNATTCTPGPWHIHRMLQAADAFPMNIGFTGKGNASLPEALREQVEAGVIGLK
                     LHEDWGTTPAAIDCCLSVAEEYDVQVAIHTDTLNESGFVEDTVAAFRGRAIHTYHTEG
                     AGGGHAPDIIKVCGEPNVLPSSTNPTRPYTVNTVDEHLDMLMVCHHLDPAIPEDVAFA
                     DSRIRRETIAAEDILHDLGAFSMLSSDSQAMG"
     gene            complement(1101..1406)
                     /locus_tag="PK144_11065"
     CDS             complement(1101..1406)
                     /locus_tag="PK144_11065"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HNF66313.1"
                     /translation="MIPGEILPEDGDIELNAGRPSVVLAVANTGDRPIQVGSHYHFFE
                     TNPALDFERPLARGMRLDIPAGTAVRFEPGQTREVTLVAYAGEGRVYGFRGDVMGEL"
     gene            complement(1559..1861)
                     /gene="ureA"
                     /locus_tag="PK144_11070"
     CDS             complement(1559..1861)
                     /gene="ureA"
                     /locus_tag="PK144_11070"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HNF66314.1"
                     /translation="MELTPREKDKLLLFTAGLLAERRRARGLKLNYPEAVALISCALM
                     EGARDGRSVAELMSWGRTLLTRADVMDGVAELIPEVQVEATFPDGTKLVTVHQPIV"
     gene            complement(1942..2781)
                     /locus_tag="PK144_11075"
     CDS             complement(1942..2781)
                     /locus_tag="PK144_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNF66315.1"
                     /translation="MTAALRSDVAGGWQARLELGFAPVAARTALVHSAHRGPLRVQRP
                     FHPEADGSCHVYLLHPPGGVVGGDGLALDVQLAPGARALLTTPSASRFYRSAGARALQ
                     RQLLRVGAGARLDWLPQETIVFDGARLASTTRLELAGDAAACAWEIVCLGRPAAGEGW
                     THGEARFGFELWRDGRPLLLEHTPCRPGSALARAAWGLGGHVTFATLVATGACSERLA
                     RLRESLGSADRLGLTHCDDLLVARYRGPDAAEARRLFTAIWRDWRTAGGGEAPAPPRV
                     WAT"
     gene            complement(3249..3908)
                     /gene="msrA"
                     /locus_tag="PK144_11080"
     CDS             complement(3249..3908)
                     /gene="msrA"
                     /locus_tag="PK144_11080"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002776604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNF66316.1"
                     /translation="MFGFGKKLTLPGAGEALPGREVAMRVPEKHYVNGHALRPPFPEG
                     FQQAMFGLGCFWGAERRFWQQPGVWSTAVGYAAGLTPNPGYEEVCSGRTGHAEVVLVV
                     WDPAETCYEALLKTFWEAHDPTQGMRQGNDVGTQYRSGIYAFDDAQLAAAEASKAAYQ
                     QALDARGLGTITTEIRPAPVFYYAEAYHQQYLAKNPGGYCGLGGTGVACPIGLDVAAG
                     G"
     gene            4130..4450
                     /locus_tag="PK144_11085"
     CDS             4130..4450
                     /locus_tag="PK144_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66317.1"
                     /translation="MKLHRSLPVLPLLAALAGSGFALAPLPAGADTVIIIRDAPPPPR
                     YERRPGARPGHVWVPGHWGWYRGHHVWRAGHWVKARPHRHYVEPAWVHTPRGWRYEPG
                     YWRR"
     gene            complement(4493..5164)
                     /locus_tag="PK144_11090"
     CDS             complement(4493..5164)
                     /locus_tag="PK144_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66318.1"
                     /translation="MYRLTKLQRMHELFCARRTPITIDGLMQELGCSRATVLQLTELL
                     RDRMGAPLEHEGAEYHYRRPEADDYTLPAMWLNAAELDTVLQLQALLGPIPPGPLAQA
                     LEPLRRRVARLVDLQPGVSGSAWRRIRVSEPGARRVQPAHMAAIAQAVLSRLRLRLEG
                     EFIAGEYSPQYLFHCRGRWLLDAWHHGAEARRTIALEEIAAAGLLDKPAVDIEEDEPP
                     FACLF"
     gene            complement(5368..5643)
                     /locus_tag="PK144_11095"
     CDS             complement(5368..5643)
                     /locus_tag="PK144_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF66319.1"
                     /translation="MNLARGAHVVEADLLAALAGGRLSAAVLDVFGQEPLPAGHALRD
                     HPEVTVTPHIAALTRIDAALDSIAAAITAIEAGATAVPGRVDPARGY"
     gene            complement(5640..6317)
                     /locus_tag="PK144_11100"
     CDS             complement(5640..6317)
                     /locus_tag="PK144_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF66320.1"
                     /translation="MQILFCHPNAAPGEAERWQAGLTARLGAAGLGVPGFTIRTPEVA
                     APPADYALLWNPPPAALLGQTRLRAAFALGAGVDALLGADVLPPGVPLYRLEDAGMAA
                     QMVEYALHAVLRWQRRFDEYEALAARGEWRTLTPRPRPLIAVLGLGALGGAVASAFAG
                     LGYRVRGWSRSPRTLAGVEGFAGPEALMACLDGAGVLIDLLPLTAGTRGLLDGRVFAR
                     LMPGRCS"
     gene            6400..8373
                     /locus_tag="PK144_11105"
     CDS             6400..8373
                     /locus_tag="PK144_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNF66321.1"
                     /translation="MSLTARLKELVVGAPRDPLVAEARRRTALLTVLAWAGLGADGLS
                     SVCYGPEEAFLALGQHTHLGLYLAVATFATVFVIALAYNQVIELFPGGGGGYKVATQL
                     LGTRAGLVSGAALMVDYVLTIAISIAASMDALFSLLPPGWGGAKRYIELTLLLLLVLL
                     NLRGYRETMRVLLPIALGFVLVHGGLLIWGIGAHAGQLPDLIRASVAETGSLTADVGW
                     LMVTGLFLKAYSLGGGTYTGLEAISNNVNRIAEPRVRNGKRTMGLMALTLAMTAGGLI
                     LLYLLWAAEPVAGQTLNAVTFRSLISTWQVGDTLPEQLLLWLVLLLEAGLLLVAANTG
                     FYSGPGMLGNMAADAWVPRHFRSLSARLVTQNGVVLMGAGAFVILLWSGGEVSLLIVL
                     YTINVFLTLSLALAGLVCHWWRERHDDPRWLRRLSLALAACCLTSGILLVTTVEKFAE
                     GGWVTVLITSAVIALCAVVRRHYDAVRDELRKVDALLAPPLGPEEDDEPRPPKVDRSA
                     ATAIFLVGQNRGIAIHAFLWVFRMFPGHFRNCVFLAVGEVDAGAVNGQNALRSLRLRT
                     ENSLHYFVRVCARNDIAAETRAAFGPDPVEELHRLTVEVMDDFPNSVCFASKLIFASD
                     SWLGPMLHNQTALSMQRRMHLDGRQMVIVPLKV"
BASE COUNT         1171 a         2982 c         2964 g         1256 t
ORIGIN      
        1 ggcccatcgc ctgcgagtcc gaggacagca tcgagaacgc gccgaggtcg tgcaggatgt
       61 cctcggcggc gatggtctcg cggcggatgc gcgaatcggc gaaggcgaca tcctcgggga
      121 tcgccgggtc gaggtggtgg cagaccatca gcatgtcgag gtgctcgtcg acggtgttga
      181 cggtgtacgg ccgcgtcggg ttggtcgagg acggcagcac gttcggctcg ccgcagacct
      241 tgatgatgtc cggcgcgtgg ccgccgccgg cgccctcggt gtggtaggtg tggatcgccc
      301 ggcccctgaa ggccgcgacc gtgtcctcga cgaagcccga ttcgttcagc gtgtcggtgt
      361 ggatcgccac ctgcacgtcg tattcctcgg ccaccgacag gcagcagtcg atcgctgccg
      421 gcgtcgtgcc ccagtcctcg tgcagcttca ggccgatgac gccggcctcg acctgctcgc
      481 gcagcgcctc gggcaggctg gcgttgccct tgccggtgaa gccgatgttc atcggaaagg
      541 catcggcggc ctgcagcatc cggtggatgt gccaggggcc gggcgtgcag gtcgtggcgt
      601 tggtgccggt cgccgggccg gtgccgccgc cgatcatcgt cgtcacgccc gacatcagcg
      661 cctcctcgat ctgctgcggg cagatgaagt ggatgtgcgt gtcgaggcca ccggcggtga
      721 cgatcagccc ctcgccggcg atgacctcgg tgccggggcc gatgacgatg ctgacgcccg
      781 gctgcacgtc ggggttgccg gccttgccga tcgcggcgat gcgcccgtcc ttgaggccga
      841 tgtcagcctt gacgatgccc cagtgatcga cgatgaccgc gttggtgatg acggtgtcga
      901 ccgcgccctg cgcgcgcgtg agctgcgact ggcccatgcc gtcgcggatc accttgccgc
      961 cgccgaactt cacctcgtcg ccgtagacgg tgtagtcggc ctcgatctcg atcagcagcc
     1021 cggtgtcgcc gaggcgcacg cgatcgcccg tggtcgggcc gtacatgtcg gcataggcct
     1081 gccggctgat gcgcgccatc tcagagttcc cccatcacgt cgccgcgaaa gccgtaaaca
     1141 cggccctcgc cggcgtaggc caccagcgtc acctcgcgcg tctgtccggg ctcgaagcgc
     1201 acggcggtgc cggccgggat gtcgaggcgc atgccgcgcg cgagcggacg ctcgaaatcg
     1261 agcgccggat tggtctcgaa gaagtggtag tgcgagccga cctggatcgg ccggtcgccg
     1321 gtgttggcga ccgcgagcac gacgctcggc cggccggcgt tcagttcgat gtcgccgtct
     1381 tcgggcagga tttcaccggg aatcatcgtc gcggcactcc tcgggaacgc aggatcgggg
     1441 cggggcggat gcgcggcgtc aggcaggacc gaccggccgt gccgccttga aacccctccc
     1501 cctcgcgggg gcaggggctg gggacagggg aaaaccgggc ttcagcgccg gaccggcatc
     1561 agacgatcgg ctgatgcacg gtcacgagct tggtgccgtc cgggaaggtc gcctcgacct
     1621 gcacctcggg gatcagctcg gcgacgccgt ccatcacgtc ggcccgcgtc agcagcgtgc
     1681 ggccccagct catcagctcg gcgacgctcc tgccgtcgcg cgcgccttcc atcagcgcac
     1741 acgagatcag cgcgacggcc tcggggtagt tgagcttcag gccgcgtgcc cggcgccgct
     1801 cggcgagcag gccggcggtg aacagcagca gcttgtcctt ctcccgggga gtgagttcca
     1861 tggttcgggc tctccgcgtc agggatgtga ggggaatcag ggatgtgatg ggaaacggtg
     1921 tgcaaacgtc agccggcgcg atcaggtcgc ccagacgcgc ggcggggccg gggcttcgcc
     1981 gccaccggcc gtgcgccagt cacgccagat cgccgtgaac agccggcgcg cctcggcggc
     2041 atcgggtcca cgatagcggg cgacgagcag atcgtcgcag tgcgtgaggc cgagccgatc
     2101 ggcgctgccg agcgattcgc gcagccgggc gagccgctcg ctgcaggcac cggtcgcaac
     2161 cagcgtggcg aaggtgacgt ggccgccgag gccccaggcc gcgcgcgcca gcgccgagcc
     2221 cggccggcac ggcgtgtgtt cgagcagcag cggccggccg tcgcgccaga gttcgaagcc
     2281 gaagcgcgcc tcgccgtgcg tccagccctc gccggccgcc ggccggccga ggcagacgat
     2341 ctcccaggcg caggcggcgg catcgccggc gagttccagc cgcgtcgtgc tcgcaagccg
     2401 cgcgccgtca aagacgatgg tctcctgcgg cagccagtcg agccgcgccc cggcgccgac
     2461 ccgcagcagc tgccgctgca gcgcccgcgc gccggcactg cggtagaagc ggctcgccga
     2521 aggtgtggtc agcagcgcgc gggcaccggg ggcgagctgc acgtcgagcg cgagcccgtc
     2581 accgccgacc acgccgcccg gcgggtgcag cagatagacg tggcagctgc cgtccgcctc
     2641 gggatggaac ggccgctgca cgcgcagcgg gccgcgatgg gcgctgtgca ccagcgcggt
     2701 gcgcgctgcg accggcgcaa agccgagttc gagccgcgcc tgccagccac cggccacatc
     2761 gctgcgcagc gcggccgtca tgcgagtcct gccgcttcag accggtgacg ggtggcaccg
     2821 gcagcgtcag gggccggtgg gccggggttg cggatcaggg catcgggcat cggacaggac
     2881 tcacgacagg gctgcgccgg ctgatacacg tttcgggccg tccgcgacaa gcccctgatc
     2941 cggcccgggg cgccgccgcc gtgagcggca cagtcgatga actttcgttt acctagtgcg
     3001 aactgcaccg gcgcgcagat ttcgcggaat tcaaaactta atttttattc atgtatttat
     3061 ccgagacagg cactcgggta tggattctga cccctgcaga ccgctcttgc gcagcgcacc
     3121 atgcctgcct acgctgcaac gcgacatctc cggatgttct cctcctgtgg tggtatctgg
     3181 ccgggcccga aaagcccggc cttttgtttg tgcgtcagcg gattacgccg gactgcgcag
     3241 tccgtccgtc agccgccggc cgccacgtcc agcccgatcg gacaggcgac gccggtaccg
     3301 cccagcccgc agtaaccgcc cgggttcttg gccagatact gctggtggta ggcctcggcg
     3361 taatagaaca ccggtgccgg gcggatctcg gtcgtgatcg tgccgaggcc gcgcgcatcg
     3421 agcgcctgct ggtaggcggc cttcgaggct tcggcggctg cgagctgcgc atcatcgaag
     3481 gcatagatgc cgctgcggta ctgggtgccg acatcgttgc cctgacgcat gccctgggtc
     3541 gggtcgtgcg cttcccagaa ggtcttcagc agcgcctcgt agcaggtttc ggccgggtcc
     3601 cagaccacca gcaccacctc ggcatgaccg gtgcgacccg agcagacctc ctcgtagccc
     3661 ggattcggcg tcaggccggc ggcgtaaccg accgcggtgc tccagacgcc gggctgctgc
     3721 cagaagcggc gctcggcgcc ccagaagcag ccgaggccga acatcgcctg ctggaagcct
     3781 tccgggaacg gcgggcgcag cgcgtggccg ttgacgtagt gcttctcggg cacgcgcatc
     3841 gcgacctcgc gcccgggcag ggcctcgccg gcgccgggca gggtgagctt cttgccgaat
     3901 ccgaacatga tgcagtcctc cgcggacggt tcgccccggc ggggatgtcc ggggtcgccc
     3961 ttcaataggg tccggggctt acgcatgcaa gggcgcagcg gacgcaggca cggccctgcg
     4021 cgtaccgcgg acgtgacacg gcgttgcgcg ccggtgaaaa cccggacacc cgaatccggt
     4081 cccattccgg tgcggtgaac gcctcaccgt gccatcggga gtcgttgcca tgaagctcca
     4141 tcgaagcctg cccgtattgc cgctgctcgc cgcgctcgcc ggcagcggct tcgcgctcgc
     4201 gccgctgccg gcgggcgcgg acaccgtgat catcatccgc gacgccccgc cgccgccgcg
     4261 ttacgagcgg cgcccgggcg cgcgccccgg ccacgtgtgg gtgccgggtc actggggctg
     4321 gtatcgcggt catcacgtct ggcgggccgg gcactgggtc aaggcacgcc cgcaccggca
     4381 ctacgtcgag ccggcctggg tgcatacgcc gcgcggctgg cgctacgagc cgggttactg
     4441 gcgccgctga gcggaccggg cgccaccgcc gtggcacaga cgcgacgccg gctcagaaca
     4501 gacaggcaaa cggcggctcg tcttcctcga tgtcgaccgc cggcttgtcc agcagtccgg
     4561 cggccgcgat ttcttcgagc gcgatcgtgc ggcgtgcctc ggcgccgtga tgccaggcat
     4621 cgagcagcca gcggccgcgg cagtggaaca ggtactgcgg cgagtattcg ccggcgatga
     4681 actcgccttc caggcgcagg cgcaggcgcg acagcacggc ttgggcgatg gcggccatgt
     4741 gcgcgggctg cacgcgccgg gcgcccggct cgctgacgcg gatgcgccgc cacgccgaac
     4801 cgctcacgcc gggctgcagg tcgaccagcc gcgccacccg ccggcgcagc ggctccagcg
     4861 cctgcgccag cggtccgggc ggaatcgggc cgagcagcgc ctgcaactgc agcacggtgt
     4921 cgagttcggc ggcgttcagc cacatcgccg gcagcgtgta gtcgtcggcc tcggggcggc
     4981 ggtagtgata ctcggcgccc tcgtgttcga gcggggcacc catgcgatcg cgcagcagtt
     5041 cggtcagttg cagcacggtc gcgcggctgc agccgagttc ctgcatcagt ccgtcgatgg
     5101 tgatcggcgt gcggcgtgca caaaacagct cgtgcatgcg ctggagcttg gtcaggcgat
     5161 acatggcgga tgtccctctc atccatccgt ggcaacgcga cacagaataa tacgatttgc
     5221 cggccattcc gtacggcaga tggcgtaatc cgccccgcct catgcctgca ggatctgtga
     5281 tccggaccgg ctgcacgcag gcccgggcct tgcggccgca gaccttgggt cagcgctgcc
     5341 agccggacgc tgcgccgccg gtgctgctca gtaaccgcgg gccggatcga cgcgaccggg
     5401 cacggccgtg gcgccagcct cgatcgccgt gatggcggcg gcgatgctgt cgagagccgc
     5461 gtcgatgcgc gtcagcgccg cgatgtgcgg cgtgaccgtg acctccggat gatcccgcag
     5521 cgcatgaccg gccggcagcg gctcctgccc gaacacatcg agcaccgccg ccgacagccg
     5581 gcccccggcg agcgcggcca gcaggtcggc ctcgaccacg tgcgcgccgc gcgcgaggtt
     5641 cacgaacagc gccccggcat cagccgcgcg aacacccggc catcgagcag gccgcgcgtg
     5701 ccggcggtca gcggcagcag gtcgatcagc acgccggcgc catcgaggca ggccatcagg
     5761 gcttccggtc ccgcgaagcc ctcgacgccg gccagcgtcc gcggcgaacg gctccagccg
     5821 cgcacgcgat agccgagtcc ggcgaaggcg gaagccaccg cgccgccgag cgcgccgagg
     5881 ccgagcacgg cgatcagcgg tcgcggccgt ggcgtcagcg tccgccattc gccgcgcgcc
     5941 gcgagcgcct cgtattcgtc gaagcgccgc tgccagcgca gcacggcgtg cagcgcgtat
     6001 tcgaccatct gcgcggccat gccggcgtct tcgagccggt acagcggcac gccgggcggc
     6061 agcacatcgg caccgagcag cgcatcgacg ccggccccga gcgcgaaggc cgcacgcagc
     6121 cgcgtctgcc cgaggagtgc cgccggcggc ggattccaca gcagcgcgta atcggccggc
     6181 ggcgctgcaa cctccggcgt ccggatcgtg aagccgggca cgcccaggcc ggccgcgccg
     6241 agccgcgcgg tcagtcccgc ctgccagcgc tcggcctcgc cgggggcggc gttcgggtga
     6301 cagaacagga tctgcatgac ctgcgtcctc gccggcggcc gggaccggct tgatagactg
     6361 gccagtctag ggggccgaac gcccgcacgg gatcaccgca tgtcgctgac agccagactc
     6421 aaggaactcg tcgtcggcgc gccgcgcgac ccgctggtcg ccgaggcgcg ccggcgcacg
     6481 gcactgctga ccgtactggc ctgggccgga ctcggcgccg acggtctttc atccgtgtgt
     6541 tacggccccg aggaagcctt cctcgcgctc ggccagcaca cgcacctcgg cctgtacctc
     6601 gccgtcgcga ccttcgcgac cgtgttcgtg atcgcgctcg cctacaacca ggtcatcgag
     6661 ctgttcccgg gcggcggcgg cggttacaag gtcgccacgc agctgctcgg cacgcgcgcc
     6721 ggcctggtgt cgggcgcggc gctgatggtc gattacgtgc tgaccatcgc catctcgatc
     6781 gccgcgtcga tggatgcgct gttctcgctg ctgccgccgg gctggggcgg cgccaagcgc
     6841 tacatcgagc tgacgctgct gctgctgctg gtgctgctga acctgcgcgg ctaccgcgag
     6901 accatgcgcg tgctgctgcc gatcgcgctc ggtttcgtgc tcgtgcatgg cggcctgctc
     6961 atctggggca tcggtgccca cgccggccag ctgccggacc tgatccgcgc ctcggtcgcc
     7021 gaaaccggct cgctgaccgc cgacgtcggc tggctgatgg tcaccggcct gttcctgaag
     7081 gcctattcgc tcggcggcgg cacctacacc ggcctcgaag cgatctcgaa caacgtcaac
     7141 cgcatcgctg aaccgcgcgt gcgcaacggc aagcgcacga tggggctgat ggcgctgacg
     7201 ctggcgatga ccgccggcgg cctgatcctg ctgtacctgc tgtgggccgc cgagccggtc
     7261 gccggccaga cgctgaacgc cgtgaccttc cgctcgctga tctcgacctg gcaggtcggc
     7321 gacacgctgc ccgagcagct gctgctgtgg ctggtgctgc tgctcgaagc cgggctgctg
     7381 ctggtggccg ccaacaccgg cttctacagc ggccccggca tgctcggcaa catggccgcc
     7441 gacgcctggg tgccgcggca tttccgctcg ctgtcggcgc ggctggtcac gcagaacggc
     7501 gtcgtgctga tgggcgccgg cgccttcgtg atcctgctgt ggtcgggcgg cgaggtgtcg
     7561 ctgctgatcg tgctgtacac gatcaacgtg ttcctgacgc tgtcgctggc gctggccggg
     7621 ctggtctgcc actggtggcg cgagcgccac gacgacccgc gctggctgcg gcggctgtcg
     7681 ctcgcgctcg ccgcctgctg cctgacctcc ggcatcctgc tggtgaccac ggtcgagaag
     7741 ttcgccgaag gcggctgggt gacggtgctg atcaccagcg ccgtcatcgc actctgcgcg
     7801 gtcgtgcgcc gtcactacga cgccgtgcgc gacgaactgc gcaaggtcga tgcgctgctc
     7861 gccccgccgc tcggccccga ggaggacgac gaaccgcgcc cgccgaaggt cgaccgcagc
     7921 gccgcgaccg cgatcttcct ggtcggccag aaccgcggca tcgccatcca cgccttcctg
     7981 tgggtgttcc ggatgttccc ggggcacttc cgcaactgcg tgttcctcgc cgtcggcgag
     8041 gtcgacgccg gcgccgtcaa cggccagaac gcgctgcgct cgctgcggct gcgcaccgag
     8101 aactcgctgc actacttcgt gcgcgtctgc gcccgcaacg acatcgccgc cgaaacccgc
     8161 gccgccttcg gccccgaccc ggtcgaggag ctgcaccggc tgaccgtcga ggtcatggac
     8221 gacttcccga acagcgtctg cttcgcctcg aaactgatct tcgcctccga ctcctggctc
     8281 ggcccgatgc tgcacaacca gaccgcgctg tcgatgcagc ggcgcatgca cctcgacggc
     8341 cggcagatgg tcatcgtgcc gctgaaggtc tga
//
LOCUS       DAOQIE010000167         8365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4341_length_8365_cov_4.054031, whole genome shotgun sequence.
ACCESSION   DAOQIE010000167 DAOQIE010000000
VERSION     DAOQIE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4341_length_8365_cov_4.054031"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..859)
                     /locus_tag="PK144_11110"
     CDS             complement(239..859)
                     /locus_tag="PK144_11110"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNF66322.1"
                     /translation="MSDAIHFYEPAQGHGLAHDPFNAIVAPRPIGWISSHDPAGRLNL
                     APYSFFNAFNYRPPIVGFASVGRKDSLNNIERTGEFGWNLVTRALATAMNASSATVAP
                     EVDEFALAGLTPVASRRIAVPRVAESPVSFECRLSQIVQLRTADGRLVPSWLVLGEVV
                     GVHIATALLVDGVYDTAAAAPLLRGGGPGDYFTIDAGARWQMTRPD"
     gene            complement(961..1248)
                     /gene="catC"
                     /locus_tag="PK144_11115"
     CDS             complement(961..1248)
                     /gene="catC"
                     /locus_tag="PK144_11115"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HNF66323.1"
                     /translation="MLYCVRMDVRVPHDVDPHRFEALKAEEKARAQLLQRDGRWRHLW
                     RVAGCYANVSVFDVTDHDELHTLLSTLPLFPYMEIVVTPLARHPSALDAAT"
     gene            complement(1324..2496)
                     /locus_tag="PK144_11120"
     CDS             complement(1324..2496)
                     /locus_tag="PK144_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HNF66324.1"
                     /translation="MSDILTPTRPALPVADLQRDLCVHSVTSHIVDVPTVRRHKLSSL
                     SVAAQSYVIVRVRLDNGVEGIGEAATLGGPRWSEECVEGIKATIDAHLAPALTGVRAD
                     AFELAGARLDAAAKRNNAAKAALETALFDAVGRSLGLPVAALLGGAVRECIPVLWTLA
                     SGDAGQELEEAEAKLAARLHDTFKVKIGAQSAAADLARLGRLAAALAGRARLIVDANQ
                     AWDETTANRCTPALAALGIELLEQPLPAWNLAGLARVRARSAVPLLADESVFTPQDML
                     ALAQAGAADAVSLKLVKHGGLLGLRRVAAVAEAAGVGLYGGCLLESSIGAAAHLHAFA
                     GLRELAWGCEHFGPQILTGDLVTEPLHYADGHVHLPSGPGLGLVLDEEQLRRYARR"
     gene            complement(2580..3431)
                     /locus_tag="PK144_11125"
     CDS             complement(2580..3431)
                     /locus_tag="PK144_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HNF66325.1"
                     /translation="MAELSMATFTDTVIGAIGPGATPRVRTVMTALIRHLHDFAREVK
                     LTTPEYRAACDFIVRIGRMSDDKRNEAVLASDVFGLESLVDVLDHHHAERGAATDSAV
                     LGPFWREHSPLLDHGASIVRGGQEGESVFVCGRVLGVNGRPVGNARIAVWETAPNGLY
                     EQQDPEQPDFNLRGQFCTDAEGRYAFRALRPIAYPIPFDGPAGELLQMMDRHPFRPAH
                     IHFRVRAPGYHELVTQIFDRDDRYLASDSVFAVKDSLTVEFRPAPAGADTRWHVAFDI
                     VLEPLAG"
     gene            complement(3611..4396)
                     /locus_tag="PK144_11130"
     CDS             complement(3611..4396)
                     /locus_tag="PK144_11130"
                     /EC_number="1.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate
                     dehydrogenase"
                     /protein_id="HNF66326.1"
                     /translation="MIHAGRFNGRIAVVTGAAQGIGRTVAVNMAREGARVALVDRSPL
                     IAEVHAEILAAGGEALAITADLERYGESRLAINEAHARFGRIDILVNNVGGTIWAKPF
                     EQYAEDEIEAEVRRSLFPTLWGCRAVLPFMIAQQAGTIVNVSSVATRGVNRVPYGAAK
                     GGVNALTACLAFECAGHGIRVLGIAPGGTEAPPRRIPRNTVAADEQTQAWYRQIVEQT
                     VESSLMKRYGTLDEQAAAILFLASDEASYITGTILPVAGGDQG"
     gene            complement(4393..5469)
                     /gene="benC"
                     /locus_tag="PK144_11135"
     CDS             complement(4393..5469)
                     /gene="benC"
                     /locus_tag="PK144_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HNF66327.1"
                     /translation="MNHRIALSFEDGVTRFVDCRAGDTLADAAYRAGINIPLDCRDGA
                     CGTCKAYCEAGRYDGGSYIDEAMSAGEAAAGYVLACQMRPQSDCVVRIAASAALCRTT
                     AATHRGRIGSVTRLSGTAVAFSIELDAPAALGFLPGQYVNVRVPGTDQKRAYSFSCGP
                     GRASVSFLLRDTAAGLLPAYLRERAEAGAAIEFEGPLGSFYLREIRRPVLMLAGGTGL
                     APFLAMLEQIAETGTDYPVHLIYGVTGDADLVELDRLDTAARRIADFTYVCCVADPAS
                     PHPNRGYVTHHLAADKLHDGDVDVYLCGPPPMVDAVRAHFAAHGIRPANFHYEKFVTS
                     GLVTAIGDSIATATAAIPETVLPQ"
     gene            complement(5504..6004)
                     /gene="benB"
                     /locus_tag="PK144_11140"
     CDS             complement(5504..6004)
                     /gene="benB"
                     /locus_tag="PK144_11140"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HNF66328.1"
                     /translation="MSGIDLSIEDVRQFLFREARYLDDREWDRWLELYAPDAEFWMPA
                     WDDDDGLTRDPQREISLIYYSRRDGLNDRVYRIRTERSGATSLPEPRTSHNINNVEII
                     DQDGSRCRVRCNWFTLYYLYKTVDTYFGTSYYTLDLSGPQPLIRNKKIVLKNDCIHHV
                     VDIYHV"
     gene            complement(6009..7355)
                     /gene="benA"
                     /locus_tag="PK144_11145"
     CDS             complement(6009..7355)
                     /gene="benA"
                     /locus_tag="PK144_11145"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase large subunit"
                     /protein_id="HNF66329.1"
                     /translation="MNAALAGQIATTLVEDREHGIHRCRRDIFTDPALFELEMQHIFE
                     GNWIYLAHDSQIPNPNDYLTTHIGRQPIFIARNRQGELNAFINACSHRGSMLCRFKKG
                     NKSTYTCPFHGWTFSNSGKLLKVKDPDGAGYPDGFSRNGSHDLTKVARFESYRGFLFG
                     SLNPDVLPLAEHLGEATKIIDMIVDQAPDGLEVLRGSSTYVYDGNWKLQAENGADGYH
                     VSSVHWNYVATTNRRKTGETADDVKAMDASSWARQKGGYYAFEHGHMLLWMQWPNPQD
                     RPLYERRDELIERFGAARADWMIGYLRNLCLYPNVYLMDQFSSQIRQIRPLAVDKTEV
                     TIYCIAPKNESPEARARRIRQYEDFFNATGMATPDDLEEFRACQMGYQGRAAQWNDMC
                     RGAQHWIDGADAGAREIGLQPVSSGVRTEDEGLYPVQHAYWVETMSKAAAAAADQE"
     gene            7871..>8365
                     /locus_tag="PK144_11150"
     CDS             7871..>8365
                     /locus_tag="PK144_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF66330.1"
                     /translation="MELRHLRYFVTVVREGNFTRAAERLHMAQPPLSRQIQQLEDRLG
                     VVLLDRQSRPLQPTEAGRFFYEQAVNVLERVETVVAQTRRYAHAEKRRFGIGFVGSTL
                     YGALPMLLRRFRAAYPQLNVELLELGSVQQVAALKDGRIDVGFGRIPVEDPAIARQLL
                     REESL"
BASE COUNT         1318 a         2726 c         2947 g         1374 t
ORIGIN      
        1 accgcgagca gccgcgcagc cggcggcggt gcggcgcggg acgggacggc gacggtctcg
       61 tcggcagcgt cggcagcgtc ggccgccgcc tcgggcggat cggaacgggc gtcgtccata
      121 aggcactcct gcggaaccgg gggcggggga acggtgggcc gggacgggaa acgatctgta
      181 atcgtccggc atgacgatga tcgcacgttg cgcagccggg gccgacggtc gcggcgcttc
      241 agtccgggcg tgtcatctgc cagcgcgcgc cggcgtcgat cgtgaagtaa tcccccggcc
      301 cgccgccgcg cagcagcggt gcggcggcag ccgtgtcgta gacaccatcg accagcagcg
      361 ccgtggcgat gtgcacgccg acgacctcgc cgagcaccag ccagctcggc accaagcggc
      421 cgtcggcggt gcgcagctgc acgatctggc tcagccggca ttcgaaggac accggacttt
      481 cggccacgcg cggcaccgcg atccggcgcg aggccaccgg cgtcagcccg gccagcgcga
      541 actcgtcgac ttcgggcgcg accgtcgcgc tgctcgcgtt catcgccgtg gccagcgcgc
      601 gcgtgaccag gttccagccg aactcgccgg tgcgctcgat gttgttcagg ctgtccttgc
      661 gaccgacgct cgcgaacccg acgatcggcg gccggtagtt gaaggcgttg aagaagctgt
      721 acggcgcgag gttcagccgc ccggccggat cgtgcgagga aatccagccg atcggccgcg
      781 gcgcgacgat ggcgttgaac ggatcgtgcg cgaggccgtg gccctgcgcg ggttcgtaga
      841 agtggatcgc atcgctcatg gtgcggactc cgggcagcag gtgcgaagac gccgcccggt
      901 cgcggaaccc gggcggcggc agggaatgcc acggtacgcc ggcgcggccg cggccggggc
      961 tcaggtcgcc gcatcgagcg ccgacggatg ccgggcgagc ggcgtcacga cgatttccat
     1021 gtacgggaac aagggcagcg tcgagagcag cgtgtgcagc tcgtcgtggt cggtcacgtc
     1081 gaagacgctg acgttcgcgt agcagccggc cacgcgccac agatggcgcc agcggccgtc
     1141 gcgctgcagc agctgcgcgc gcgccttttc ctcggccttc agggcttcga accggtgcgg
     1201 gtccacatcg tgcggcacgc gcacgtccat ccggacgcag tacagcatcg gaatcacctc
     1261 tcgaaatggt tcggggttta aggaaacagg aggaaacagg gcaatgccgc cggcgccgcg
     1321 gcttcagcgg cgcgcgtagc ggcgcagctg ctcctcatcg agcacgaggc cgaggccggg
     1381 accggacggc aggtgcacgt ggccgtcggc gtagtgcagc ggctcggtga cgaggtcgcc
     1441 ggtcaggatc tgcggcccga agtgctcaca gccccatgcg agttcgcgca ggcccgcgaa
     1501 cgcgtgcagg tgcgcggccg cgccgatcga gctttcgagc aggcagccgc cgtacaggcc
     1561 gacgccggcg gcctcggcca cggccgcgac gcggcgcagg ccgagcaggc cgccgtgctt
     1621 gacgagcttc agcgacaccg catcggccgc gccggcctgc gcgagcgcga gcatgtcctg
     1681 cggcgtgaac acgctttcgt cggcgagcag cggcaccgcc gagcgcgccc gcacacgcgc
     1741 gagcccggcg agattccagg ccggcagcgg ttgttcgagc agctcgatgc cgagggcggc
     1801 gagtgccggc gtgcagcggt tcgcggtcgt ctcgtcccag gcctgattgg catcgacgat
     1861 cagccgcgcg cggccggcca gcgccgcggc gagccggccg agccgcgcca gatcggccgc
     1921 ggccgactgc gccccgatct tgaccttgaa ggtgtcgtgc aggcgcgcgg cgagcttggc
     1981 ctcggcctcc tcgagctcct ggccggcgtc gccggaggcg agcgtccaca gcaccgggat
     2041 gcactcgcgc accgcgccgc cgagcagggc cgcgaccggc aggccgaggc tgcggccgac
     2101 ggcgtcaaac agcgccgtct ccagcgcggc cttggcggcg ttgttgcgct tcgcggccgc
     2161 atcgagccgc gcgccggcga gctcgaaggc gtcggcgcgc acgccggtca gcgccggcgc
     2221 gaggtgggcg tcgatcgtcg ccttgatgcc ttccacgcat tcctcgctcc agcgcgggcc
     2281 gccgagcgtg gccgcctcgc cgatgccttc gacgccgttg tcgaggcgca cgcgcacgat
     2341 cacgtagctc tgcgcggcga ccgacagcga ggacagcttg tgccggcgca cggtcggcac
     2401 gtcgacgatg tggctcgtga ccgaatgcac gcagaggtcg cgctgcaggt ccgccaccgg
     2461 cagcgccggg cgggtcgggg tcaggatgtc ggacatgggt gagactccgg agctggccgg
     2521 tccgggcgga aatgcccgga ccggggacgc cgccgtgacg gcagcgggcg gggctgcgtt
     2581 cagccggcga gcggttccag cacgatgtcg aaagcgacgt gccagcgcgt gtcggccccg
     2641 gccggggccg gccggaattc gacggtcagc gaatccttga ccgcgaacac cgaatcgctc
     2701 gccagataac gatcgtcgcg gtcgaagatc tgcgtgacca gttcgtgata acccggtgcg
     2761 cgcacgcgga aatggatgtg ggccgggcgg aacggatggc ggtccatcat ctgcagcagt
     2821 tcgccggccg gaccgtcgaa cggaatcgga tacgcgatcg ggcgcaaggc gcgaaatgcg
     2881 tagcggccct cggcatcggt gcagaactgc ccgcgcaggt tgaaatccgg ctgctccgga
     2941 tcctgctgct cgtagagtcc gttcggtgcg gtttcccaga ccgcgatccg cgcattcccg
     3001 accggccggc cgttgacgcc gagcacgcgc ccgcagacga ataccgattc gccctcctga
     3061 ccgccgcgca cgatcgacgc gccatgatcg agcagcggcg aatgctcgcg ccagaacggc
     3121 ccgagcacgg cgctgtcggt ggcggccccg cgttcggcat ggtgatggtc gaggacatcg
     3181 acgaggcttt cgagtccgaa cacgtcgctc gcgagcacgg cttcgttgcg cttgtcgtcg
     3241 gacatccggc cgatgcgcac gatgaaatca caggccgcgc ggtattcggg cgtcgtcagt
     3301 ttcacctcgc gggcaaaatc gtgcaggtgg cggatcagcg ccgtcatgac ggtgcgcaca
     3361 cgcggcgttg cgccgggacc gatcgcgccg atgacggtgt cggtgaaggt agccatcgat
     3421 aattcagcca tttcaagttc tcctctggtg gtggatattc ggcggatgta cgggcattcc
     3481 cggatttccg cgcttcggat gtaaaaattc atgtggcacg ctgcattcat cgacctggca
     3541 gcgaccggtg ttccggctgc cggggggctg cggatttcct gtccgcatgt ccctgccccg
     3601 gcaatggctt tcagccctga tcgccgccgg cgaccggcag gatcgtgccg gtgatgtacg
     3661 aggcttcgtc cgaggcgaga aacaggattg ccgcggcctg ttcgtcgagc gtgccgtagc
     3721 gtttcatcag cgaggattcg acggtctgtt cgacgatctg ccggtaccac gcctgcgttt
     3781 gttcatccgc ggccaccgta ttgcgcggaa tgcgccgtgg tggtgcctcg gtgccgccgg
     3841 gcgcaatgcc aagcacgcgg atgccgtggc ccgcgcattc gaacgcgagg caggcggtca
     3901 gcgcattgac gccgcctttc gccgcgccgt acggcacgcg attgacgcca cgcgtcgcca
     3961 ccgaggacac gttaacgatg gtgccggcct gctgcgcgat catgaacggc agcaccgcgc
     4021 gacagcccca gagcgtcgga aacagcgagc ggcggacttc ggcctcgatt tcgtcctcgg
     4081 cgtactgctc gaacggcttt gcccagatcg tgccgccgac gttgttgacg aggatgtcga
     4141 tgcggccgaa gcgcgcgtgc gcctcgttga tcgcgagtcg ggattcgccg tagcgttcga
     4201 gatcggccgt gatcgccagg gcctcgccgc cggcggcgag gatttcggcg tggacttccg
     4261 cgatcagcgg cgagcggtcg acgagtgcga cgcgcgcacc ttcgcgcgcc atgttcacgg
     4321 ccaccgtgcg gccgatgccc tgcgccgcac cggtgacgac ggcgatcctg ccattgaaac
     4381 gaccggcatg gatcattgcg gcagcaccgt ttcggggatg gctgccgtgg cggtggcgat
     4441 gctgtcgccg atggcagtca cgaggccgct ggtcacgaat ttctcgtaat ggaaattcgc
     4501 cggccggatg ccgtgtgcag caaagtgcgc gcgcacggca tcgaccatcg gcggcggccc
     4561 gcacagatac acgtccacgt cgccgtcgtg cagtttgtcg gccgccaggt gatgggtgac
     4621 gtaaccgcgg ttcggatgcg gactggccgg atcggcgacg cagcacacat acgtgaaatc
     4681 ggcgatgcgc cgtgctgccg tgtcgagccg gtcgagttcg accagatcgg catcgccggt
     4741 cacgccgtaa atcaggtgca ccggataatc cgtgccggtt tcggcgatct gctcgagcat
     4801 cgccagaaac ggcgcgagtc cggtgccgcc ggcgagcatc agcaccggcc ggcggatttc
     4861 gcgcagatag aaactgccga gcggcccctc gaattcgatc gccgcaccgg cttcggcgcg
     4921 ttcgcgcaga taagccggca gcaggccggc cgcggtgtcg cgcagcagga acgacacgct
     4981 cgcgcgcccc gggccgcagc tgaatgaata cgcgcgcttc tggtcggtgc ccggcacgcg
     5041 gacgttgacg tactggcccg gcaggaagcc gagcgcggcc ggcgcatcga gctcgatcga
     5101 gaatgcgacg gcggtgccgg agagccgcgt gaccgaaccg atgcggccgc ggtgcgtggc
     5161 cgcggtggtc cggcacagcg cggcactcgc cgcgatgcgc acgacgcagt ccgactgcgg
     5221 gcgcatctgg caggccagca cgtagccggc tgcggcctct ccggcgctca tggcctcgtc
     5281 gatgtagctg ccgccgtcgt agcggccggc ttcgcagtaa gccttgcagg tgccgcaggc
     5341 accgtcgcgg cagtcgagcg gaatgttgat cccggcccgg taagccgcat cggcgagcgt
     5401 gtcgccggcc cggcaatcga cgaagcgcgt gacgccgtct tcgaaactca gggcgatccg
     5461 gtgattcatg gcgttctcct cctccagtcg tccgtcatcg gcgtcagacg tgatagatgt
     5521 cgacgacgtg atggatgcag tcgtttttca gcacgatctt cttgttccgg atcagcggct
     5581 gcggaccgga cagatcgagc gtgtaatacg aggtgccgaa atacgtatcg acggtcttgt
     5641 agaggtaata cagcgtgaac cagttgcagc gcacccggca ccggctgccg tcctgatcga
     5701 tgatctcgac gttgttgatg ttgtgcgagg tgcgcggctc cggcaggctg gttgcaccgg
     5761 aacgctcggt ccggatgcgg tagacgcgat cgttcagacc gtcccggcgc gagtaataga
     5821 tcagcgaaat ctcgcgctgc ggatcgcggg tcaggccgtc gtcgtcatcc caggccggca
     5881 tccagaattc agcgtccggc gcatacagtt caagccagcg atcccattcg cgatcgtcga
     5941 gataacgcgc ctcgcgaaac agaaactggc gcacgtcctc gatggaaaga tcgatgccgc
     6001 tcatggattc actcctggtc cgcagccgca gcggcggcct tgctcattgt ttcgacccag
     6061 taggcgtgct ggaccggata cagaccttcg tcctcggtgc gcacgccgct ggacaccggc
     6121 tgcagaccga tttcacgggc accggcgtcg gccccgtcaa tccagtgctg cgcgccgcgg
     6181 cacatgtcgt tccattgcgc cgcccgcccc tgatacccca tctggcaggc gcggaattcc
     6241 tcaagatcgt cgggcgtggc catgccggtg gcgttgaaga agtcctcgta ctggcggatg
     6301 cgccgggcgc gcgcctcggg gctttcgttc ttcggcgcga tgcagtagat cgtgacttcg
     6361 gtcttgtcga cggcaagcgg ccggatctga cggatctgcg aactgaactg gtccatcaga
     6421 tacacgttcg ggtacaggca gaggttgcgc agataaccga tcatccagtc ggcccgcgcc
     6481 gcgccgaaac gctcgatcag ttcgtcgcgc cgttcgtaca gcggccggtc ctgcggattc
     6541 ggccactgca tccacagcag catgtggccg tgctcgaacg cgtaataacc gcctttctgc
     6601 ctggcccagc tgctcgcatc catcgccttc acatcgtcgg cggtttcgcc ggtcttgcgc
     6661 cggttggtgg tcgcgacgta attccagtgc accgaactga cgtgatagcc gtcggcgccg
     6721 ttttcggcct gcagcttcca gttgccgtcg tagacgtagg tcgaggaacc gcgcagcact
     6781 tcgagtccgt ccggggcctg atcgacgatc atgtcgatga tcttcgtcgc ctcgccgaga
     6841 tgttcggcga gcggcagcac gtccgggttc aggctgccga acaggaagcc gcggtaggat
     6901 tcgaagcgtg cgaccttcgt caggtcgtgc gagccgttgc ggctgaaacc gtccggatag
     6961 ccggccccgt ccggatcctt gaccttcagc agcttgccgg agttactgaa ggtccagccg
     7021 tggaacgggc aggtgtaggt ggacttgttg cctttcttga accggcagag catcgagccg
     7081 cggtggctgc aggcgttgat gaaggcattg agttcgccct ggcggttgcg ggcgatgaag
     7141 atcggctggc ggccgatgtg cgtggtcaga taatcgttgg gattcggaat ctggctgtcg
     7201 tgggcaaggt agatccagtt gccctcgaag atatgctgca tttccagttc gaacagcgct
     7261 ggatcggtga agatgtcgcg gcggcaacgg tgaatgccgt gttcgcgatc ttcgaccagt
     7321 gtcgtggcga tctgtccggc aagggctgca ttcatcgtga tgttctcctc gttcggtgtc
     7381 tgggaacggt tgcagcaccg gtggccgtca gcgcggcggt cggcacgaac cgttgaagac
     7441 ggggctggaa tgcctgccga tgcggtgaac ggcatttcat ttccgcgccg gcggaccggc
     7501 ctgtcccgtt tccggtttct actgcgggga ccatcatcat caggccggac acggccgtcc
     7561 aagaccgaat gagtccgccc cgataccctg cgggtatcgg ggcgaagggc ggttttttcg
     7621 ggatgccgca gatgcgtgaa aatccgggca gatcacgaat ccgggcggat tcgcaggtgc
     7681 cgaccgtgcc gtgcggcggc ggaatgcggt gggcggcgtc ccttgcgcaa tgcagtatcc
     7741 ggagccggct gcgcggcggt gcgcatgggc gcctagcatg gaagcagcag cggtggccgg
     7801 accatcgtcg tccggccgcc gcggcgtcac gtcgtcagct cattccctcg acagcagcgc
     7861 ggtccgtacc gtggaactcc gacacctgcg ttacttcgtg accgtcgtgc gcgagggcaa
     7921 cttcacgcgc gcggccgagc ggctgcacat ggcgcagccg ccgctgagcc ggcagatcca
     7981 gcaactggag gaccggctcg gcgtcgtgct gctcgatcgg cagagcaggc cgctgcagcc
     8041 gaccgaggcc ggacgcttct tctacgaaca ggcggtgaac gtgctcgaac gcgtcgaaac
     8101 cgtcgtcgcg cagacgcggc gctacgccca tgccgagaag cgccgcttcg gcatcggctt
     8161 cgtcggctcg acgctgtacg gggcgctgcc gatgctgctg cgccgcttcc gcgccgcgta
     8221 cccgcaactg aacgtcgagc tgctcgaact cggcagcgtg cagcaggtcg cggcgctgaa
     8281 ggacggacgc atcgatgtcg gcttcggacg catcccggtc gaggacccgg cgatcgcgcg
     8341 ccagctgctg cgcgaggaaa gcctg
//
LOCUS       DAOQIE010000168         8347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4356_length_8347_cov_7.367342, whole genome shotgun sequence.
ACCESSION   DAOQIE010000168 DAOQIE010000000
VERSION     DAOQIE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4356_length_8347_cov_7.367342"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..824)
                     /locus_tag="PK144_11155"
     CDS             complement(192..824)
                     /locus_tag="PK144_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNF66331.1"
                     /translation="MNRLPLTLYMTPGSCSTGIHILLEEIGVPFAAHIVNLPKGEHRQ
                     PAYLAINPRGTIPALRLEDGSALAGFTAIAYWLARRFPKAGLLPAEETAVARVLEALD
                     YVTGTIHGQGFTRIFTTDAYTTDPALQPAVQARGREIVAEGFALLAVQLGGRDWLVGE
                     TFSIADAALFYVEFWADHLGLPLPAHCRAHFERLRARPAVQQVLREEGYR"
     gene            complement(916..2112)
                     /locus_tag="PK144_11160"
     CDS             complement(916..2112)
                     /locus_tag="PK144_11160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005966369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF66332.1"
                     /translation="MRAFERSLADFPAPSSEALAVCARLVDQIAADIAAAGGRLRFDH
                     YMQRALYAPGLGYYAAGSRKFGAGGDFTTAPELSPLFSRCLARAGAPVLAALGGGDVL
                     EFGAGTGTMAAGVLAELARLDCLPGRYRILDASAELRERQRATLAAKVPALLDRVEWL
                     DALPPAGSLRGVVLGNELLDAMPVRRFRIGADGPQELYVRGDAATGFELVAGPADAGC
                     RAAVAAIEAAVGPLAEGYESEWNPALPAWFASLGETLAAGAAILIDYGHPRRAYYHPS
                     RARGTFLCHYRHRAHDDALVWPGLQDLTAHVDFTAVADAALAAGFEVAGYCAQAWFLF
                     GCGLEALLAEVDPEDLPRHVEAVRQVKLLTLPGEMGERFQAIGLTKGIGLRLPAFALR
                     DERGRL"
     gene            2181..2930
                     /locus_tag="PK144_11165"
     CDS             2181..2930
                     /locus_tag="PK144_11165"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNF66333.1"
                     /translation="MEDITGDARVALVTGAARRVGAEIVRTLHATGWRIALHYRSAAA
                     EAETLASELNARRTASVATFAADLLDTAALPGLVEAAATVWGRLDLLVNNASSFYATP
                     VGQATEAHWDDLVGSNLKAPFFLSQAAAPWLRAHEGCIVNIVDLHAGRPMDEHPIYCA
                     AKAGLASLTRSLARELAPEVRVNGIAPGAILWPEHSIDAGERERILGRVPLGRAGSPA
                     DIARTLLFLVQYAPYVTGQIIAVDGGRTTGV"
     gene            2993..3685
                     /locus_tag="PK144_11170"
     CDS             2993..3685
                     /locus_tag="PK144_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HNF66334.1"
                     /translation="MRRPTLAALCLLCTLLTLLPVASAPAKTVRYVSDSLEVPLRAGA
                     SLRHRIVRMLPSGTQVEVLETDTDKGFALVRTADGTVQGWLPNQYLMETPSARTALGT
                     MQTEFDRVRSENASLKERLDTLTTQKVDAETSFSQLSSDNHRLAQELATIRRTAANAI
                     AIDQQNKDLQERVVNLERELQIVQQENQSLADRSNRDWFLLGAGVLVSGVLVGLILPR
                     LRVQRRSRWGEL"
     gene            complement(3691..4050)
                     /gene="folB"
                     /locus_tag="PK144_11175"
     CDS             complement(3691..4050)
                     /gene="folB"
                     /locus_tag="PK144_11175"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNF66335.1"
                     /translation="MDIIFIRDLSIETTIGVYEWERGIRQTIVLDLELGTDVRRSAAS
                     GALEDALDYMAVATRLGAYVGGERWELVETLAERIAELLITEFHVPWLRLTLTKPAAV
                     RGARAVGVRIERSAGGG"
     gene            4208..4807
                     /gene="plsY"
                     /locus_tag="PK144_11180"
     CDS             4208..4807
                     /gene="plsY"
                     /locus_tag="PK144_11180"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNF66336.1"
                     /translation="MTSALLCLVFAYLCGSVSTAILVCRLMGLPDPRSEGSRNPGATN
                     VLRIGGRKAAAITLAGDFLKGLIPVLVARAFGQGPEVLAATAFAAFLGHLYPVFFGFQ
                     GGKGVATALGVMVGLSWQVALAALATWLVIARVWKISSLAALGAATLSPLYALLFGLP
                     GAYALALLPMVALLLWRHRENIRRIRAGTESRIGDKKKT"
     gene            complement(4819..5844)
                     /gene="tsaD"
                     /locus_tag="PK144_11185"
     CDS             complement(4819..5844)
                     /gene="tsaD"
                     /locus_tag="PK144_11185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNF66337.1"
                     /translation="MIVLGIETSCDETGVALYDGARGLRAHALYSQVALHAEYGGVVP
                     ELASRDHVRKLVPLLDEAFAAAGLERGAVEGIAYTAGPGLIGALMVGASFARALAAAW
                     QVPALGVHHMEGHLLAPMLEADPPAFPFLALLVSGGHTLLVAVAGVGRYRILGESIDD
                     AAGEAFDKTAKLLGLPYPGGPALARLAGRGDPARFTFPRPMTDRPGLDFSFSGLKTHA
                     LNTLASLSGSEEDRADVARAFEDAVVDTLFLKCRRALRETGLKRLVVAGGVGANERLR
                     EQLAALMQARGGRAYYPRPAFCTDNGAMIAYAGHCRLAAGEREDGPIRAVPRWPLDAL
                     QPPPALA"
     gene            5969..6184
                     /gene="rpsU"
                     /locus_tag="PK144_11190"
     CDS             5969..6184
                     /gene="rpsU"
                     /locus_tag="PK144_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNF66338.1"
                     /translation="MPVVKVKENEPFDIALRRFKRACEKAGILAEIRAREFYEKPTQE
                     RKRKQAAAVKRHQKKLAREVARRTRLY"
     gene            6209..6655
                     /locus_tag="PK144_11195"
     CDS             6209..6655
                     /locus_tag="PK144_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNF66339.1"
                     /translation="MSLKARLTEDMKDAMRAKDKDRLATIRLILAALKQREVDERIEL
                     DDTQVLAVLDKMLKQRRESIAQFESAGRTDLAEKEAAEAAIIQTYLPAALSPAELDAL
                     IDATITATGAAGVRDMGKVMAAIKAQAQGRADMSEVSARIRTRLGG"
     gene            6766..>8347
                     /gene="dnaG"
                     /locus_tag="PK144_11200"
     CDS             6766..>8347
                     /gene="dnaG"
                     /locus_tag="PK144_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNF66340.1"
                     /translation="MSGRIPQGFIDQLLARTDLVEVIGARVPLKKQGREFAACCPFHN
                     EKSPSFYVSPSKQFFHCFGCGAHGNAIGFLMDYEHLAFPEAVEELARSAGIEVPREGG
                     SAAAGQQDQYAEILHWLARADQWFRQQLRHHPERARPIEYLRGRGLAGALAAEFGIGY
                     APPERDALLQALLAEGATRKLLLAAGLVAERDGGTLVDRFRGRVMFPIRDRRGRTIAF
                     GGRVLGDGQPKYLNSPETPVFHKGRELYGLYEARMAARQIGRLVVVEGYMDVVMLAQH
                     GLRCAVATLGTSTTAEHAEKLFRITHDIVFCFDGDRAGREAAWRALENVLPQVRDGRR
                     IGFLFLPDGEDPDSLVQQEGAEAFAARLDGALPLTDYLAERLRAQVNLNTDEGAARLM
                     ELAKPLLERMPDSPARDLLVQALDRGSVLRRHTRLQQAPAGVGEFRRKVDYRKSPVRH
                     AIALLLAEPALAAEAGERASYARIDAPGVDLLLELIDIFRDSPHLPAGAAVERYRGTA
                     RQRPLERLLAWAPEVPANE"
BASE COUNT         1232 a         3015 c         2976 g         1124 t
ORIGIN      
        1 acctcgtgcg ccggccgctg gccgctgccg agcgcgccgc gctcggcgag gccttcgccg
       61 cggtgttcgt ggctgcggcc gggctgctgc tgctcggcct gctgctgtgg tcgcgcgtag
      121 ccgaacggcc gctgcgcggg gcggacgctg cgccaatcag cgccgaaacc tgacccgggc
      181 agcgcaccgg ctcagcgata gccttcctcg cgcagcacct gctgcacggc cggccgcgcg
      241 cgcaggcgct cgaagtgcgc gcggcagtgc gccggcagcg gcaggccgag gtgatcggcc
      301 cagaactcga cgtagaacag cgcggcatcg gcgatcgaga aggtctcgcc gaccagccag
      361 tcgcggccgc cgagctgtac cgcgagcagc gcgaagcctt cggcgacgat ctcgcgcccg
      421 cgcgcctgca cggccggctg cagcgccggg tcggtcgtgt aggcatcggt cgtgaagatg
      481 cgcgtgaagc cctgaccgtg gatcgtgccg gtcacgtagt cgagcgcttc gagcacccgc
      541 gccacggcgg tttcttcggc cggcagcagc ccggccttcg ggaagcgccg cgccagccag
      601 tacgcgattg cggtgaagcc ggcgagcgcg ctgccatctt cgaggcgcag cgccggaatc
      661 gtgccgcgcg ggttgatcgc cagatacgcc ggctggcgat gctcgccctt cggcaggttg
      721 acgatgtgcg cggcgaaggg cacgccgatc tcttcgagca ggatgtggat gccggtcgag
      781 cacgaaccgg gcgtcatgta cagcgtcagc ggcaaacggt tcatggcggc caccggtagc
      841 gggagtcagg gggacgatcg gaggcggcgg cagcgacggc gagcgccccg agggacggca
      901 ggcatgcgcc cggcctcaca gccgcccccg ctcgtcgcgc agcgcgaacg ccggcagacg
      961 caggccgata cccttcgtca gcccgatcgc ctggaagcgc tcgcccatct cgccgggcag
     1021 cgtcagcagc ttgacctgac gcacggcctc gacgtggcgc ggcaggtctt cgggatcgac
     1081 ctcggcgagc agcgcttcca ggccgcagcc gaacaggaac caggcctgtg cgcagtagcc
     1141 ggcgacctcg aagccggcgg cgagcgcggc atcggcgacg gcggtgaaat cgacgtgcgc
     1201 ggtcaggtcc tgaagccccg gccagaccag cgcgtcgtcg tgggcgcggt ggcggtagtg
     1261 gcacaggaag gtgccgcgcg cgcgcgacgg atggtaatag gcgcggcgcg ggtggccgta
     1321 gtcgatcagg atcgcggccc cggcggcgag cgtctcgccg aggctcgcaa accacgccgg
     1381 cagcgccgga ttccattccg attcgtagcc ctcggcgagc ggcccgaccg cggcctcgat
     1441 cgccgcgacc gcggcccggc agccggcatc ggcggggccg gcgaccagtt cgaagccggt
     1501 tgcggcatca ccgcggacgt acagctcctg cgggccgtcg gcgccgatgc gaaagcgccg
     1561 caccggcatc gcatcgagca gttcgttgcc gagcacgaca ccgcgcaggc tgccggccgg
     1621 cggcagtgca tcgagccatt cgacgcgatc gagcagcgcc ggcaccttcg cggccagcgt
     1681 tgcgcgctgg cgctcgcgca attcggcgct ggcatcgagg atgcggtagc gcccgggcag
     1741 gcagtcgagc cgcgccagtt cggcgagcac gccggcggcc atcgtgccgg tgccggcgcc
     1801 gaattcgagc acgtcgccac cgccgagtgc ggcgagcacc ggcgcgccgg cacgcgccag
     1861 acagcgcgag aacagcggcg agagttccgg cgcggtcgtg aaatccccgc cggcgccgaa
     1921 cttgcggctg ccggcggcgt agtaaccaag gccgggcgcg tacagcgcgc gctgcatgta
     1981 gtgatcgaag cgcagccgcc cgccggcagc ggcgatgtcg gcggcgatct gatcgaccag
     2041 ccgggcgcag acagccagcg cctcggatga gggcgccgga aaatcggcga gggagcgttc
     2101 gaaggcgcgc atggagcttt ccgcagcggg cacaagtcgg cgaagctagc cgcaaccgac
     2161 tgcgcgaggg ggcgagggcc gtggaggata tcaccgggga tgcacgcgtc gcgctggtca
     2221 ccggcgcagc gcgccgggtc ggcgccgaga tcgtgcgcac gctgcacgcg accggctggc
     2281 gcatcgcgct gcactaccgc agcgccgccg ccgaggccga gacgctcgct tccgaactga
     2341 acgcccggcg cacggcctcg gtcgcgacct tcgccgccga cctgctcgac accgcggcgc
     2401 tgcccgggct ggtcgaggcg gcggcgacgg tctggggccg gctcgacctg ctggtcaaca
     2461 acgcctcgtc gttctacgcg acgccggtcg ggcaggcgac cgaggcgcac tgggacgatc
     2521 tggtcggctc gaacctgaag gcaccgttct tcctgagcca ggccgcggcg ccgtggctgc
     2581 gcgcgcacga gggctgcatc gtcaacatcg tcgacctgca cgccggccgg ccgatggacg
     2641 agcacccgat ctactgcgcc gccaaggcag gtctggcctc gctgacgcgc tcgctggcac
     2701 gcgagctggc cccggaggtg cgcgtcaacg gcatcgcgcc gggcgcgatc ctgtggccgg
     2761 agcactcgat cgacgccggc gagcgcgagc gcatcctcgg gcgcgtgccg ctcgggcgcg
     2821 ccggttcgcc ggccgacatc gcgcgcacgc tgctgttcct ggtgcagtac gcgccctacg
     2881 tgaccgggca gatcatcgcc gtcgacggcg ggcgcacgac cggcgtctga agccgggtcg
     2941 gggtacactc gccggctctg tgccgcatgc cccgttgtga ggaagtcccc cgatgcgccg
     3001 tccgacgctc gccgcgctct gcctgctctg caccctgctc acgctgctgc cggtcgcctc
     3061 cgcgccggcg aagaccgtcc gctacgtcag tgacagcctc gaagtgccgc tgcgcgccgg
     3121 cgccagcctg cgccaccgga tcgtgcgcat gctgccgagc ggcacgcagg tcgaggtgct
     3181 ggagaccgac accgacaagg gtttcgcgct ggtgcgcacg gccgatggca ccgtgcaggg
     3241 ctggctgccg aaccagtacc tgatggaaac gccgtcggcg cgcacggccc tcggcaccat
     3301 gcagaccgag ttcgatcgcg tgcgctccga gaacgccagc ctcaaggaac gcctcgacac
     3361 gctgaccacg cagaaggtcg atgccgagac gagcttctcg cagctgtcct ccgacaacca
     3421 ccggctggcg caggaactgg cgacgatccg caggaccgcc gccaacgcca tcgccatcga
     3481 ccagcagaac aaggacctgc aggagcgcgt cgtcaacctg gagcgcgagc tgcagatcgt
     3541 gcagcaggag aaccagtcgc tggccgatcg cagcaacagg gactggttcc tgctcggggc
     3601 cggcgtgctg gtctcgggcg tgctggtcgg gctgatcctg ccgcggctgc gcgtgcagcg
     3661 gcgcagccgc tggggcgagc tctgagcgaa tcagccgccg ccggcgctgc gctcgatgcg
     3721 cacgccgacg gcgcgcgcac cgcgcaccgc ggccggcttg gtcagcgtca gccgcagcca
     3781 cggcacgtgg aactccgtga tcagcagctc ggcgatgcgc tcggcgagcg tctcgaccag
     3841 ttcccagcgc tcgccgccga cgtaggcgcc gaggcgcgtg gcgaccgcca tgtagtcgag
     3901 cgcatcctcc agcgcaccgc tggcggcgct gcggcgcacg tcggtgccga gttcgaggtc
     3961 gagcacgatg gtctggcgga tgccgcgctc ccattcgtag acgccgatcg tggtctcgat
     4021 gctcaggtcg cggatgaaga tgatgtccat tcgccggctc ggtggctttt gcgagaagat
     4081 ccatgatcgc cggaaacgac cgccaatgct cccccgcgca tcgccgccgc aggagcgccg
     4141 gccatcgccg tccgtgttcc tccttccctg cggcttcccg ccgcctccca ggactcctgc
     4201 tccgcccatg accagcgccc tgctctgcct cgtgttcgcg tatctgtgcg gctcggtctc
     4261 gaccgcgatc ctcgtctgcc ggctcatggg cctgcccgat ccgcgctcgg agggctcgcg
     4321 caaccccggc gcgaccaacg tgctgcgcat cggcggcagg aaggccgccg cgatcacgct
     4381 ggccggtgac ttcctgaaag ggctgatccc ggtgctggtc gcgcgcgcct tcgggcaggg
     4441 gccggaagtg ctggcggcga cggcgttcgc ggccttcctc ggccatctgt acccggtgtt
     4501 cttcggcttc cagggcggca agggcgtggc gaccgcgctc ggcgtcatgg tcgggctgtc
     4561 gtggcaggtg gcgctggccg cgctcgcgac ctggctggtg atcgcgcggg tctggaagat
     4621 ttcctcgctc gcggcactcg gcgccgcgac gctgtcgccg ctgtacgcgc tgctgttcgg
     4681 cctgcccggg gcctacgcgc tggcgctgct gccgatggtc gcgctgctgc tgtggcgcca
     4741 ccgcgagaac atccgccgca tccgcgccgg caccgaaagc cgcatcggcg acaagaagaa
     4801 gacctgatcc ggccccggct acgcgagggc cggcggcggc tgcagcgcat cgagcggcca
     4861 gcgcggcacg gcgcggatcg ggccgtcctc gcgctcgccg gccgcgagcc ggcagtgacc
     4921 ggcataggca atcatcgcgc cgttgtcggt gcagaaggcc gggcgcgggt aataggcccg
     4981 gccgccgcgc gcctgcatca gcgcggcgag ctgctcgcgc aggcgctcgt tggcaccgac
     5041 gccgccggcc accaccagcc gcttcaggcc ggtctcgcgc agcgcccgcc ggcacttcag
     5101 gaacagcgtg tcgacgacgg catcctcgaa ggcacgggcg acatcggccc ggtcctcctc
     5161 gctgccggag aggctggcga gcgtgttcag cgcgtgggtc ttcaggccgc tgaagctgaa
     5221 atccagcccc ggccggtcgg tcatcggccg cgggaacgtg aagcgcgccg gatcgccgcg
     5281 tccggccagc cgcgccagcg ccggcccgcc cggatacggc aggccgagca gcttggcggt
     5341 cttgtcgaag gcctcgccgg cggcatcgtc gatcgattcg ccgaggatgc ggtagcgccc
     5401 gacgccggcc acggcgacca gcagcgtgtg gccgccggag acgagcagcg cgaggaacgg
     5461 gaaggccggc ggatcggctt cgagcatcgg cgcgagcaga tggccttcca tgtgatgcac
     5521 gccgagcgcc ggcacctgcc aggccgcggc cagcgcacgc gcgaagctcg ccccgaccat
     5581 cagcgcgccg atcagcccgg ggccggcggt ataggcgatg ccttcgaccg cgccgcgttc
     5641 gagcccggcg gcggcgaagg cctcgtcgag cagcggcacg agcttgcgca cgtgatcacg
     5701 cgaggcgagc tccggcacca ccccgccata ttcggcgtgc agcgccacct ggctgtagag
     5761 cgcgtgcgcg cgcagaccgc gcgcgccgtc gtagagcgcg acgccggtct cgtcgcagga
     5821 ggtttcgatg ccgagcacga tcatcgcggg attgtccggg cgggcgccgg cacgacgcaa
     5881 gccgcttttg caatggtccg gacaaaggct tagactcgcg cgctcgccgt taccggccca
     5941 gcccgctgcc tatcgaggta actccttaat gcccgtcgtg aaagtgaagg aaaacgagcc
     6001 gttcgacatc gccctgcgcc gcttcaagcg cgcgtgcgag aaggccggca tccttgccga
     6061 gatccgtgcc cgcgagttct acgagaagcc cacccaggaa cgcaagcgca agcaggcggc
     6121 cgcggtcaag cgccaccaga agaagctcgc gcgcgaagtc gcccgtcgca cccgcctgta
     6181 ctgattccgc tccggacacc cccgccgcat gtcgctgaaa gcccgcctca ccgaggacat
     6241 gaaggacgcg atgcgcgcga aggacaagga ccgtctcgcg acgatccgcc tgatcctcgc
     6301 cgcgctgaag cagcgcgagg tcgacgagcg catcgagctc gacgacacgc aggtcctcgc
     6361 cgtcctcgac aagatgctca agcagcggcg cgagtcgatc gcgcagttcg agagcgccgg
     6421 ccgcaccgat ctggctgaaa aggaggccgc cgaggccgcc atcatccaga cctacctgcc
     6481 ggccgcgctg agccccgccg aactcgatgc tttgatcgac gcgaccatca ccgccaccgg
     6541 cgcggccggc gtgcgcgaca tgggcaaggt catggccgcc atcaaggcgc aggcgcaggg
     6601 ccgggcggac atgtccgagg tcagcgcccg catcaggacc cggctcggcg gctgatcccg
     6661 cccgtgcccg atggccccga gaacccggcc cggcgccggg ttctcgcgtt ttcgcggcca
     6721 ttcacgcgct agtctcgctg caaccgcgcc gcctggaatc accgcatgtc cggtcgcatc
     6781 ccgcagggct tcatcgacca gctgctcgcc cgcaccgacc tcgtcgaggt catcggcgcg
     6841 cgcgtgccgc tgaagaagca gggacgcgaa ttcgccgcgt gctgcccgtt ccacaacgag
     6901 aagtcgccgt cgttctacgt gagtccgtcc aagcagttct tccactgctt cggctgcggc
     6961 gcccacggca acgccattgg cttcctgatg gactacgagc atctggcctt cccggaagcg
     7021 gtcgaggaac tcgcgcgcag cgccggaatc gaggtgccgc gcgagggcgg cagcgcagcg
     7081 gccggccagc aggatcagta cgccgaaatc ctgcactggc tcgcgcgcgc cgatcagtgg
     7141 ttccgccagc agctgcgcca tcacccggag cgggcccggc cgatcgaata cctgcgcggc
     7201 cgcggcctcg ccggcgcgct cgccgccgag ttcggcatcg gctacgcgcc gccggagcgc
     7261 gacgcgctgc tgcaggcgct gcttgccgag ggcgcgacgc gcaagctgct gctcgccgcc
     7321 ggcctggtcg ccgagcgcga cggtggcacg ctggtcgatc gcttccgcgg gcgcgtgatg
     7381 ttcccgatcc gcgaccggcg cggacgcacg atcgccttcg gcggtcgcgt gctcggcgac
     7441 ggccagccga agtacctgaa ttcgccggaa acgccggtgt tccacaaggg ccgcgaactc
     7501 tacggcctgt acgaggcgcg catggccgcg cgccagatcg gccggctggt ggtggtcgag
     7561 ggctacatgg acgtggtcat gctcgcgcag cacggcctgc gctgcgcggt cgccacgctc
     7621 ggcacctcga ccacggccga gcacgccgag aagcttttcc gcatcacgca cgacatcgtc
     7681 ttctgcttcg acggcgaccg cgccggacgc gaggccgcct ggcgcgcgct ggagaacgtg
     7741 ctgccgcagg tgcgcgacgg ccgacgcatc ggcttcctgt tcctgcccga tggcgaggac
     7801 ccggattcgc tggtacagca ggagggcgcc gaagccttcg ccgcccggct cgatggcgcg
     7861 ctgccgctga ccgactacct cgccgaacgg ctgcgcgcgc aggtcaacct gaacaccgac
     7921 gaaggcgcgg cgcggctgat ggaactggca aagccgctgc tcgaacgcat gccggattcg
     7981 ccggcgcgcg acctgctggt gcaggcgctc gatcgcggtt cggtgctgcg ccggcacacg
     8041 cgcctgcagc aggccccggc cggcgtcggc gagttccggc gcaaggtcga ttaccgcaag
     8101 agcccggtgc gccacgccat cgcgctgctg ctcgccgagc ccgcgctggc cgccgaggcc
     8161 ggcgagcgcg cgagctatgc acggatcgat gcacccggcg tcgatctctt gcttgaactg
     8221 attgatatct tcagggacag cccgcatcta ccggcaggtg ccgcagtcga acgctatcgt
     8281 ggaaccgccc ggcagcggcc gctggagcgc ctgctggcct gggcaccgga ggtgccggcc
     8341 aacgagt
//
LOCUS       DAOQIE010000169         8322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4376_length_8322_cov_8.269868, whole genome shotgun sequence.
ACCESSION   DAOQIE010000169 DAOQIE010000000
VERSION     DAOQIE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4376_length_8322_cov_8.269868"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PK144_11205"
     CDS             complement(<1..694)
                     /locus_tag="PK144_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNF66341.1"
                     /translation="MSNATVTLKDNASGKTVDMPIYTPTHGDPVVDIGALNKEFGYFT
                     YDPGFVSTASCKSAITYLDGDKGELLYRGHPIEQLAEQCTHLEVSYLLLNGELPTAEQ
                     LKNFEYTISHHTLIKDNLLKFFDGFNYDAHPMAMLTGVVGSLAGFYHDNLNILDPEHR
                     KITAHRLIAKLPTIAAACYKHNIQQPFMYPRNDLSYCGNLLHMMFSTPCEEFEIDPVA
                     EKALDVLFILHAD"
     gene            complement(1123..1365)
                     /locus_tag="PK144_11210"
     CDS             complement(1123..1365)
                     /locus_tag="PK144_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNF66342.1"
                     /translation="MKKGIHPEYREVVFQDLSADFSFLTRSTIRTKDKITWTDGKEYP
                     LVKVEVSSASHPFYTGKQKIVDTAGRVDRFRRKYGL"
     gene            1585..2904
                     /gene="rimO"
                     /locus_tag="PK144_11215"
     CDS             1585..2904
                     /gene="rimO"
                     /locus_tag="PK144_11215"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNF66343.1"
                     /translation="MANTEPRVGFVSLGCPKALVDSEQILTRLRAEGYAISPDYRGSD
                     LVVVNTCGFIDAAIEESLDAIGEALTENGRVIVTGCLGARPERITEVHPKVLAVTGPH
                     ALEEVMGAVHEHLPKPHDPFLDLVPPAGIKLTPRHYAYLKISEGCNHRCTFCIIPQMR
                     GDLVSRPVGDVLLEAENLVRAGVQELLVISQDTSAYGVDVRYRTGFWGGRPVKTQMSA
                     LAEALGTLGVWVRLHYVYPYPSVDDVIPLMAEGKILPYLDVPLQHASPRVLKAMKRPG
                     DIDGTLARIRRWREQCPELTLRSTFIVGFPGETDDDFERLLDFLEEAELDRVGCFAYS
                     PVEGAPANALDGAVPEALKQERLARFMEVQAGISAAKLEQRIGSECELLIDGYAEDGR
                     AVGRSAAEAPEIDGIVAIEDSSHCRPGQRVRVRIIDADEHDLYAELL"
     gene            complement(2929..4653)
                     /locus_tag="PK144_11220"
     CDS             complement(2929..4653)
                     /locus_tag="PK144_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66344.1"
                     /translation="MGLFILSRRERPDPLQPAWLAAHRRHGFREPLRLTAGDWRLDLF
                     GALNGLGPQLVQRDNGDFAACTGVFFYRGARGTAGLESLLDDFDGERFPWSGSRGHFA
                     VIVCKAGRMFLATDALGGYKVYHDGGRTLLSSSFTALRANLAQATPDAQGAWEYAWNG
                     ATYGTRTFIREIRQLRRGSLLTLGPQAEISAEYRLPAADAPQHASIDEAAEDYAARLR
                     ALFRVYAAGGGRFRTALSGGYDSRLMLALLLDAGLDPQVYVYGRAGDDDVRVAKAIAA
                     GEGLALEHVDKSQTAPPPSAGHTERAWVRFDGWKNDGLFDSGVDAEDRLLRVPGDVSV
                     LNGSAGEVFRNFFYLPDRRYRARDVVWSFYSRHDPQAMTGAFDAEAYDTAMSADLLAA
                     IGTTAPDDQALTRADIEALYPLHRARYWTARDVGLNQRFGPLLFPYLEPAVFSGTERE
                     PIAWKDYGRLEAKIIERVHPALARYPSAYGFVPADGPDLRYRLKMQLTFLRPTWLRRY
                     SYRLQQRGPLPPRPAWLGDAALSAAGLDPALPHMRALFVPERLRDAEVFNRVCTMELA
                     LAGQPLPG"
     gene            complement(4756..5025)
                     /locus_tag="PK144_11225"
     CDS             complement(4756..5025)
                     /locus_tag="PK144_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator, Rof"
                     /protein_id="HNF66345.1"
                     /translation="MHIDIDDADPAADPYVPIDCELYSRYELAIMHRRTLRLVWRDEA
                     GGTHLETVLPCDLQTRMHQEFLLAETLDGEPRCLRLDRIRHVDFL"
     gene            complement(5027..5215)
                     /locus_tag="PK144_11230"
     CDS             complement(5027..5215)
                     /locus_tag="PK144_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HNF66346.1"
                     /translation="MLESWFVDCPWCGEGFEADIDTSAGDAEYVEDCRVCCAPVVFRI
                     RVAADGSLLAVETLREGD"
     gene            complement(5229..6392)
                     /gene="hemW"
                     /locus_tag="PK144_11235"
     CDS             complement(5229..6392)
                     /gene="hemW"
                     /locus_tag="PK144_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNF66347.1"
                     /translation="MPNFTAPIPLAVYVHLPWCVRKCPYCDFNSHAATGPLPEAAYVD
                     ALLADLEHELPGVWGRRVDTVFIGGGTPSLFSPEAIERLLAGLRARLPLRPDAEITLE
                     ANPGSVEAGRYAELRAAGITRLSIGVQSFDDDQLGRLGRIHGRREAMRAAEAAHAAGY
                     PDGFNLDLMYGLEQQTPATALADLAIAIDLEPAHLSHYQLTLEPNTLFHARPPVLPDD
                     DTVADIEAACRARLEARGYERYEISAYAQPGRRCRHNLNYWEFGDYLGLGAGAHGKRT
                     DPHAGEIRRTRKARNPRDYLETAPRGTAGHEETVPEAELPFEFLLNALRLIDGVPAPL
                     YAERTGLALEALEAPLATARARGLLEADPQRLRASAHGLQFVNDLLQLFMTPR"
     gene            6594..7196
                     /locus_tag="PK144_11240"
     CDS             6594..7196
                     /locus_tag="PK144_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66348.1"
                     /translation="MSMDKSMVLLKTVKGLDEIQNKVHGLAPKRRRVLIMVDGRMTVA
                     EMLERLAALGSDLEAQLESLLSEGFLAPAGAASARAAPAPGAAPALKPAALDASPLPG
                     QVEAVIPQFAEFNLDKAKGFARYVVLGALGPVGGKRAARIEQTRSAGELRAELDELRD
                     VLPKLMPKRQAEQVWKQLEPLMLSITEAPSVSAMPQTLPP"
     gene            complement(7193..7813)
                     /gene="rdgB"
                     /locus_tag="PK144_11245"
     CDS             complement(7193..7813)
                     /gene="rdgB"
                     /locus_tag="PK144_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNF66349.1"
                     /translation="MNDVPSGPRRVVLATGNAGKVRELAACLADLGCEVLPQSHFGVP
                     EAEETGLSFVENAILKARNAAAHTGLPAIADDSGIEVDALDGAPGIYSARFAGPGADD
                     AANNARLLAELAGVPPARRSARYRCVLVYLRHARDPMPLICAGTWEGTVLSAARGSGG
                     FGYDPLFQPADCTVTAAELALADKNRLSHRGRALAALHAALAAGAR"
     gene            7957..>8322
                     /locus_tag="PK144_11250"
     CDS             7957..>8322
                     /locus_tag="PK144_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66350.1"
                     /translation="MHPDVILSRTGLAQTEIRDRTRQLTPRLRRLLLLVDGHTSVAGT
                     VERYGGLFGPDIGTLIDDLHSRGYLAGTAMTAAGDDAPLVPADTPLTLDAARERALEL
                     LATVVGPVKSTGHAYRPSVA"
BASE COUNT         1284 a         2772 c         2994 g         1272 t
ORIGIN      
        1 ggtcggcgtg caggatgaac agcacgtcga gtgccttctc ggcgaccgga tcgatctcga
       61 actcctcgca gggagtcgag aacatcatgt gcagcaggtt tccgcagtac gacaggtcgt
      121 tgcgcggata catgaacggc tgctggatgt tgtgcttgta gcaggccgcg gcgatggtcg
      181 gcagcttcgc gatcaggcgg tgcgccgtga tcttgcggtg ctccggatcg aggatgttca
      241 ggttgtcgtg atagaagccg gcgagcgagc cgacgacgcc ggtcagcatc gccatcgggt
      301 gcgcgtcgta gttgaagccg tcgaagaact tcagcaggtt gtccttgatc agcgtgtgat
      361 ggctgatcgt gtactcgaag ttcttcagct gctcggcggt cggcagttcg ccgttgagca
      421 gcaggtagct gacttcgagg tgcgtgcact gctcggcaag ctgctcgatc gggtggccgc
      481 ggtacagcag ttcacccttg tcgccgtcga ggtaggtgat cgcgctcttg cacgaggcgg
      541 tcgacacgaa gcccggatcg tacgtgaagt aaccgaattc cttgttgagg gcgccgatgt
      601 ccacgacggg gtcaccgtgc gtgggggtgt agatcggcat gtcgacggtc ttgccggacg
      661 cattgtcctt cagggtgacg gtcgcgttgg acattcggct acctccatta ttcggattca
      721 gtgcgggaga tgctgcaagg cccggccctg tcgggcgact tcctgcatgc cggctgccgc
      781 ggagttcggg aggtcccgag gccgatggcc ccggaccgga cgactccgca tccggtcgca
      841 tcacgcagcg cacgggtgaa cgtcgctggc ggatgcggac cggaccccca tttcagctgg
      901 catgccacgc cccgggatcc gggcggggcg ggatcataaa ccgaagcgtc acatcctgtc
      961 ccggcctttc gatgcctcat tgcgaaaacc gcaatgctgg atccatgtat ttgaaatatc
     1021 agttttttta taggctctcg ccggattccg ccggccggcg gaaggcaaga aaaaaggcat
     1081 cgcgaggatg cctttttccg gatgccgcag gatttcctgc gcttacaggc cgtacttgcg
     1141 gcggaagcgg tcgacgcggc cggcggtgtc gacgatcttc tgcttgccgg tatagaacgg
     1201 gtgcgaggcg ctggagactt cgaccttgac cagcggatat tccttgccgt cggtccaggt
     1261 gatcttgtcc ttggtccgga tcgtcgagcg ggtcaggaac gagaagtccg ccgacaggtc
     1321 ctggaacacc acttcgcggt attcggggtg gatgcctttc ttcatgaacg tcaaaccttg
     1381 cgtgcgtgag ccggtggacg acgcgggcac gcgccggtcc gggaagccgt gcaccccgtc
     1441 gggggcgaca aaggggcggt acgataagca aatcgtgccg ggcacgcaag catcgccgcc
     1501 ggccggggct tgccgccacc ggcaggctcc ggtataagcc gcgcgcttgc gcggcgtccg
     1561 taaccgcttt accggaaaga aaaaatggcc aacaccgaac cccgggtcgg cttcgtcagc
     1621 ctcggctgcc cgaaggcgct cgtcgattcc gaacagatcc tgacccggct gcgggccgag
     1681 ggctacgcga tctcgcccga ctatcgcggc tccgatctgg tcgtcgtcaa cacctgcggt
     1741 ttcatcgatg ccgcgatcga ggagtcgctc gatgcgatcg gcgaggcgct gaccgagaac
     1801 ggccgcgtca tcgtcaccgg ctgcctcggc gcccggcccg agcgcatcac cgaggtgcat
     1861 ccgaaggtgc tggcggtgac cggcccgcat gcgctcgaag aagtcatggg ggcggtgcat
     1921 gaacatctgc cgaagccgca cgatccgttt ctggatctcg tgccgccggc cggcatcaag
     1981 ctgacgccgc gccattacgc ctacctgaag atttccgaag gctgcaacca tcgctgcacg
     2041 ttctgcatca tcccgcagat gcgcggcgat ctggtcagcc gcccggtcgg cgacgtgctg
     2101 ctggaggccg agaacctcgt gcgcgccggc gtgcaggaac tgctggtgat ctcgcaggac
     2161 accagtgcct acggcgtcga cgtgcgctac cgcaccggct tctggggcgg gcggccggtg
     2221 aagacgcaga tgagcgcgct cgccgaggcg ctcggcacgc tcggcgtctg ggtgcggctg
     2281 cactacgtct atccgtaccc gagcgtcgat gacgtgatcc cgctgatggc ggaagggaag
     2341 atcctgccct acctcgacgt gccgttgcag cacgcgagcc cgcgcgtgct gaaggccatg
     2401 aagcggcccg gcgacatcga cggcacgctg gcgcgcatcc gccgctggcg cgagcagtgc
     2461 ccggaactca cgctgcgcag caccttcatc gtcggcttcc cgggcgagac cgatgacgac
     2521 ttcgagcgcc tgctcgactt cctcgaagag gccgaactcg atcgggtcgg ctgcttcgcg
     2581 tattcgccgg tcgagggggc tccggccaac gcgctcgacg gcgcggtgcc cgaggcgctg
     2641 aagcaggagc ggctggcgcg cttcatggaa gtgcaggccg gcatcagcgc cgcgaagctc
     2701 gaacagcgca tcggcagcga gtgcgagctg ctgatcgacg gctacgccga ggatggccgt
     2761 gcggtcggac gcagcgccgc cgaggcgccg gagatcgacg gcatcgtcgc gatcgaggac
     2821 agcagccact gccggccggg ccagcgcgtg cgcgtgcgca tcatcgatgc cgacgagcac
     2881 gacctgtacg ccgaactgct ctgacgccca tgcccgcccg ctgcggcctc agccgggcag
     2941 cggctggccg gcgagcgcga gttccatcgt gcagacgcgg ttgaacacct cggcatcgcg
     3001 aagcctttcc ggcacgaaca gcgcgcgcat gtgcggcagc gccggatcga ggccggcggc
     3061 gctgagcgcg gcatccccga gccaggccgg gcgcggcggc agcgggccgc gctgctgcag
     3121 ccggtagctg tagcggcgca gccaggtcgg gcgcaggaag gtcagctgca tcttcagccg
     3181 gtagcgaaga tccgggccgt cggccggcac gaagccgtag gcgctcggat agcgcgcgag
     3241 cgccggatgc acgcgctcga tgatcttcgc ctccagccgt ccgtagtcct tccacgcgat
     3301 cggctcgcgc tcggtgccgc tgaacacggc cggctccaga tacgggaaca gcagcggccc
     3361 gaagcgctgg ttcaggccga catcacgcgc ggtccagtag cgcgcccggt gcagcggata
     3421 cagcgcctcg atgtcggcgc gggtgagcgc ctggtcgtcg ggcgccgtcg tcccgatcgc
     3481 cgccagcagg tcggcgctca tcgccgtgtc gtaggcttcg gcatcgaagg cgccggtcat
     3541 cgcctgcggg tcgtggcgcg agtagaacga ccagaccacg tcgcgcgccc ggtagcgccg
     3601 gtcgggcagg tagaagaagt tgcggaacac ctcgccggcc gagccgttca gcaccgagac
     3661 atcgcccggc acgcgcagca ggcggtcctc ggcatcgacg ccgctgtcga acaggccgtc
     3721 gttcttccag ccgtcgaagc gcacccaggc gcgctcggtg tggccggccg agggcggcgg
     3781 tgcggtctgc gacttgtcga cgtgttcgag cgcgaggcct tcgccggcgg cgatggcctt
     3841 ggcgacgcgc acgtcgtcgt ccccggcgcg gccgtagacg tagacctgcg gatccagccc
     3901 ggcatcgagc agcagcgcca gcatcagccg cgagtcatag cccccggaga gcgcggtgcg
     3961 gaagcgcccg ccgccggccg cgtagacgcg aaacagcgcg cgcagccgcg cggcgtagtc
     4021 ctcggcggcc tcgtcgatcg aggcgtgctg cggtgcatca gcggccggca gccggtattc
     4081 ggcgctgatt tccgcctgcg gtccgagggt gagcaggctg ccgcggcgca gctggcggat
     4141 ctcgcggatg aaggtccggg tgccgtaggt cgcgccgttc caggcgtatt cccaggcgcc
     4201 ctgggcatcg ggcgtcgcct gcgcgagatt cgcgcgcagc gccgtgaagc tgctcgacag
     4261 cagcgtgcgt ccgccgtcgt ggtagacctt gtagccgccg agcgcgtcgg tggcgaggaa
     4321 catccgcccg gccttgcaga cgatcaccgc gaaatggccg cggctgccgg accacggaaa
     4381 gcgctcgcca tcgaagtcat cgagcaggga ttcgaggccg gccgtgccgc gcgccccccg
     4441 gtaaaagaac accccggtgc aggcggcgaa gtcgccgttg tcgcgctgca cgagttgcgg
     4501 gccgaggccg ttcagcgccc cgaacaggtc gagccgccag tcgccggccg tgaggcgcag
     4561 cggctcgcgg aagccgtggc gccggtgcgc ggccagccat gccggctgca gcggatcggg
     4621 gcgctcgcgc cgcgacagga tgaacagacc cacggacgat cccccggcag gccccggagc
     4681 ggggcggggc ggcactatcc gggaagccgc cggccgcgtg caagcgcacc gccggcgtcc
     4741 gggccggccg cggggctaca ggaagtcgac gtggcggatg cggtccagcc gcaggcagcg
     4801 cggttcgccg tccagggttt cggcgagcag gaattcctgg tgcatgcgcg tctgcaggtc
     4861 gcagggcagc acggtttcga ggtgcgtgcc gccggcctcg tcgcgccaga ccagccgcag
     4921 cgtgcgccgg tgcatgatcg ccagctcgta gcggctgtag agctcgcagt cgatcggcac
     4981 atacgggtcg gcggccgggt cggcgtcatc gatgtcgatg tgcatgtcag tcgccctcgc
     5041 gcagggtttc gacggcgagc agcgagccgt cggcagcgac gcggatgcgg aacacgaccg
     5101 gtgcgcagca gacccggcag tcctcgacgt attcggcatc gccggcggag gtgtcgatgt
     5161 ccgcctcgaa gccttccccg caccacggac agtcgacgaa ccaggattcg agcatcggtg
     5221 cagtcccttc agcgcggggt catgaacagc tgcagcagat cgttcacgaa ttgtaggccg
     5281 tgcgcgctgg cgcgcaggcg ctgtggatcg gcttcgagca ggccgcgcgc gcgcgccgtc
     5341 gccagcggcg cttcgagcgc ttcgagcgca agcccggtgc gttcggcata cagcggcgcc
     5401 ggcacaccgt cgatcaggcg cagcgcgttc agcaggaact cgaacggcag ctcggcttcc
     5461 ggcacggttt cctcgtggcc ggccgtgccg cgcggggcgg tttccaggta atcgcgcggg
     5521 ttgcgggcct tgcgcgtgcg gcggatctcg ccggcgtgcg ggtcggtgcg cttgccgtgg
     5581 gcaccggcac cgaggccgag gtagtcgccg aactcccagt agttcaggtt gtgccggcag
     5641 cggcggccgg gctgcgcgta ggcggagatt tcataacgct cgtagccgcg ggcttcgagg
     5701 cgcgcgcggc aggcggcctc gatgtcggcg accgtgtcgt catcgggcag caccgggggc
     5761 cgggcgtgga acagcgtgtt cggctccagc gtcagctggt agtgcgacag gtgcgccggc
     5821 tccagatcga tcgcgatcgc gagatcagcg agcgccgtgg ccggcgtctg ctgctccagg
     5881 ccgtacatca gatcgaggtt gaagccgtcg ggatagcccg cggcgtgtgc ggcctcggcg
     5941 gcgcgcatcg cctcgcgccg gccgtggatg cgcccgagcc ggccgagctg gtcgtcatcg
     6001 aagctctgca cgccgatcga cagccgcgtg atgccggcgg cgcgcagttc cgcgtagcgc
     6061 ccggcctcga ccgagcccgg attggcttcg agcgtgatct cggcatccgg gcgcagcggc
     6121 aggcgcgcgc gcaggccggc gagcaggcgc tcgatcgctt cgggcgagaa caggctcggc
     6181 gtgccgccgc cgatgaatac ggtatcgaca cgccgccccc agacgcccgg cagttcgtgt
     6241 tcgaggtcgg ccagcagcgc atcgacgtag gcggcttccg gcagcgggcc ggtcgcggcg
     6301 tgcgagttga agtcgcagta cgggcacttg cgcacgcacc acggcaggtg cacgtatacg
     6361 gcgagcggga tcggggcggt gaagttcggc atggcgtagg gcccgggtga cggacgaaac
     6421 ggaatcacgg cgccgcattt gttcctggcg ccgggctttg tcatgatttg aaacttttca
     6481 ttaccagaat ccggaagccg cgcaccggca gcgcgcagtg aaccggaccc ccagggacgc
     6541 cggatcggca cctatcaagg gacatacggg gcaagcggag gaggactcgc ggcatgagca
     6601 tggacaagtc gatggttttg ctgaaaaccg tcaagggact cgatgaaatc cagaacaagg
     6661 tccatggcct ggcgccgaaa cgccggcgcg tgctgatcat ggtcgacggc cgcatgacgg
     6721 tcgccgagat gctcgagcgg ctcgccgccc tcggcagcga tctggaagcc cagctcgaaa
     6781 gcctgctgag cgaagggttt ctggcgccgg ccggtgcggc atcggcgcgc gcggccccgg
     6841 caccgggcgc tgcgccggcc ctgaagccgg ccgccctcga tgcctccccg ctccccgggc
     6901 aggtcgaggc cgtgatcccg cagtttgcgg agttcaacct cgacaaggcc aagggcttcg
     6961 cccgctacgt cgtgctcggc gcgctcggcc cggtcggcgg caagcgtgcc gcgcgcatcg
     7021 agcagacgcg ctcggccggc gagctgcgcg ccgaactcga cgagctgcgc gacgtgctgc
     7081 cgaagctgat gccgaagcgc caggccgagc aggtctggaa gcagctcgaa ccgctgatgc
     7141 tgtcgatcac cgaggcgcca tcggtcagcg cgatgccgca gaccctgccg ccctaacgtg
     7201 cgcctgccgc cagcgccgcg tgcagcgccg ccagcgcgcg gccgcggtgg ctcagccggt
     7261 tcttgtcggc gagcgccagt tcggcggccg tcaccgtgca gtcggcgggc tggaacagcg
     7321 ggtcgtagcc gaagccgccg ctgccgcgcg ccgcgctgag caccgtgcct tcccaggtgc
     7381 cggcgcagat cagcggcatc gggtcgcggg cgtggcgcag atacaccagc acgcagcggt
     7441 agcgcgcgct gcgtcgtgcc gggggcacgc cggccagctc cgccagcagc cgggcgttgt
     7501 tggcggcgtc atcggcgccc ggcccggcga agcgcgccga atagatgccc ggcgccccgt
     7561 cgagcgcgtc gacctcgatg ccggagtcat cggcgatcgc cggcaggccg gtgtgtgcgg
     7621 cggcgttgcg cgccttcagg atcgcgttct cgacgaagct cagcccggtc tcctcggctt
     7681 cgggcacgcc gaagtgcgac tgcggcagga cttcgcagcc gagatcggcg aggcaggcgg
     7741 ccagctcgcg caccttgccg gcgttaccgg tcgcgagcac gacgcggcgc ggtccggacg
     7801 gtacatcgtt catcggaatt tcctcgttct gaatcagggt ttcgctcggc ttgttgcatt
     7861 gcagcggccg gttcatgatc cggctgcccg ggacacggaa aaaataaccg ggcagcacgg
     7921 cttcagacag accgatcaga ccgagggccg cccgccatgc accccgatgt catcctgagc
     7981 cggaccggtc tcgcacagac cgaaatccgc gatcgcaccc gtcagctcac cccccgcctg
     8041 cgccgcctgc tgctgctcgt ggacgggcac accagcgtcg ccggcaccgt cgagcgctac
     8101 ggcggcctgt tcggcccgga catcggcacg ctgatcgatg acctgcacag ccgtggctac
     8161 ctggccggca cggcgatgac cgcggccggc gacgacgcgc cgctcgtgcc ggcggacacg
     8221 ccgctgacgc tcgacgccgc gcgcgagcgc gcgctcgaac tgctggccac ggtggtcggg
     8281 ccggtgaagt cgacggggca cgcgtacaga ccctcggtcg cg
//
LOCUS       DAOQIE010000170         8315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4380_length_8315_cov_7.648305, whole genome shotgun sequence.
ACCESSION   DAOQIE010000170 DAOQIE010000000
VERSION     DAOQIE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_8315_cov_7.648305"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK144_11255"
     CDS             complement(<1..186)
                     /locus_tag="PK144_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66351.1"
                     /translation="MHAFRGWHSLDRPVPDAVQAALLPPAGSVVAPGTTRSAPGTVLC
                     AEHPGALAHDEAGRCALL"
     gene            complement(249..929)
                     /locus_tag="PK144_11260"
     CDS             complement(249..929)
                     /locus_tag="PK144_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF66352.1"
                     /translation="MPKPSAELPALLLGMLLLAGPLAGFAQQNCGSLENAFGPYNYND
                     PLIRQSGYGGSKGTIIDLVEGAHLRTFESLSRPDFETMGSIDYTLRAIPNNPRALFLL
                     IRLHRKLNGNLPKPPRVVGDSSAWPGTPECYFERAMRFVPRDPIVRQLLGVYLHWNGK
                     YQQALDAYGQATDLGLKSAELYYNIGLAHFELKQYAEAKDNAHKAYDMGYPSPGLRNK
                     LGSVGQWP"
     gene            complement(910..1977)
                     /locus_tag="PK144_11265"
     CDS             complement(910..1977)
                     /locus_tag="PK144_11265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF66353.1"
                     /translation="MSWTFLPAATGFARQRAAWAALNEAGRRSPLLDPDFVEPLLALP
                     GHGTPVLAIRGEASAPQALLLLERQGLGRWRSFVPMQMPITPLVCQPGLDAGTLIGEL
                     ITALPALLMSLDLLQLDPQFDPRPGPQAGYHELDYIETARITIDAPFESWWAGRSKNL
                     RQNLNKQRNRFEREGVCLRLECVRDPAGVAAAIADYGRLESAGWKSEGGTAVSPDNAQ
                     GHFYREVLERFCRRGQGRIYRYFYDDRLVTTDLCIEHDGTLIVLKTTYDESIQGSSPA
                     MLMRQAYLAELFADPGLRTIEFYGRAMDWHRRLTDDLRTIYHRGYIRWPQLRRLVHWL
                     RDARHGTASPRETLEHAETER"
     gene            complement(1974..3173)
                     /locus_tag="PK144_11270"
     CDS             complement(1974..3173)
                     /locus_tag="PK144_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF66354.1"
                     /translation="MPHTHLPVVPVAPILNLDARPPLETHPVPSVLDAGDVRLLTAGR
                     LGECHALRLLGIGPGDRVLLPAYHCISMLYPIQWLGAEPVFYRLHPDTSIDFEDMAAH
                     LAAGAKAVIATHFFGFAQDIGRVRALCDAHDAALIEDCAHAFFGQWQGRPLGSFGDYA
                     IASPVKFFPVFDGGCLISARHSLEAVRPRTGDAAFQIKAVLDPLERATLYGRLSLLGS
                     LLWLKNTLWTALKRRRGRTDGAAAPVIGPSSSGGGAELALEWLETGMSRAARLGMQRG
                     RRASARIAQARRANYRRLAAALSGLPGCRPLRPELPDETVPYVLPLLVDAPAQVFPAL
                     KQARVPVLRWEDMPEAALTACPVANRYRSALLQLPCHQALRSHEIDWIAAQVRAAVAA
                     AGTGVPA"
     gene            complement(3337..5385)
                     /gene="fusA"
                     /locus_tag="PK144_11275"
     CDS             complement(3337..5385)
                     /gene="fusA"
                     /locus_tag="PK144_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF66355.1"
                     /translation="MAYTTRDIRNLALLGQSGAGKTSLAEALLVAAGRIAVAGSVEKG
                     TTVCDSDPLEKAQGRSLDAALVSFDRDGCHVNLLDTPGAPDFLGQAIAVLPAVETAAI
                     VVNAQAGIEPVTQRMMARAAERGLCRLIVVNKIDADGVDLPGLLAQLQETFGRECLPL
                     NLPAQGAGAVVDCFFNPAGEADFPSVAAAHQALIDQVVEVDEALMATYLEQGEELDPA
                     QLHAPFEQALREGHLIPVCFVSARTGAGIPELLDILTRLAPNPGEGNPPACLLQRGDE
                     PAVPYAVEPDPAKHVVAHVFKVLIDPFVGKLALFRIHQGTVTKDSQLYIGDGRKPFKV
                     GHLFTLFGRDHPEVPNGIPGDIRAVAKIDDIHRDAILHDSHDEDYLHLAPSRFPMPVA
                     GIALAARTHNDENRLADVLHRLLEEDPCLTLEANAATRETVLRGLSDQHLRSTLDKMR
                     ERYRLEVDTRPPRIAYRETLQARAEGHYRHKKQTGGAGQFGEVHLRVEPLERGAGFEF
                     ASEVVGGAIPGQFIPAVEKGVREACASGAIAGYPVVDVRVVVTDGKHHPVDSKEVAFA
                     TAGREAFLAAIREARPVVLEPIVDLDVQIPQDAMGSVTGDLSGRRGRILGTRTLNAGR
                     VAVVAQAPLAELDDYPARLKAMTGGSGGYTLELARYEAVPPAVQKELIAQYRPRAEDA
                     "
     gene            complement(5473..5991)
                     /locus_tag="PK144_11280"
     CDS             complement(5473..5991)
                     /locus_tag="PK144_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNF66356.1"
                     /translation="MNTRFEVIEADITKLAVDAIVNAANASLLGGGGVDGAIHRAAGP
                     QLYEECRMLGGCPTGEARVSAGYRLPAHWVIHTVGPVWRGGTENEAALLASCYRESLR
                     LAVGIGAASIAFPAISCGIYGYPLDQATAIAVASARAHAEAHAQPQRIVFCCFGSAAF
                     ETYRRALGAVAH"
     gene            6159..7436
                     /gene="serS"
                     /locus_tag="PK144_11285"
     CDS             6159..7436
                     /gene="serS"
                     /locus_tag="PK144_11285"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNF66357.1"
                     /translation="MLDPRLLRSELDDTARRLARRGYVLDTGRIRGLEEQRKATQVRA
                     QELQAVRNSRSKAIGKARGAGEDIAPLLAEVASLGDELKAAETALAALQAELDEIYSG
                     IPNLPDDSVPDGRDEQDNVELRRWGTPRQFDWTPLDHADLLPGFLDFESAAKLTGARF
                     SVLHGPLARLHRALIQFMLDLHTVEHGYSEVYVPYLVNADTLRGTGQLPKFEADLFRL
                     SGEFPYYLIPTAEVPVTNLARGEILEAATLPRQYAAHTPCFRSEAGSYGKDVRGLIRQ
                     HQFEKVELVHLVRPDDSFAALERLTAHAETVLQRLELPYRVIVLCTGDMGFGAVRTHD
                     IEVWLPGQGKYREISSCSNMGDFQARRMQARWRNPATGKPELLHTLNGSGLAVGRTLV
                     AILENYQQADGSVTMPAALKPYLGGLESIRPKR"
     gene            7705..8262
                     /locus_tag="PK144_11290"
     CDS             7705..8262
                     /locus_tag="PK144_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66358.1"
                     /translation="MISMKQPSLVLHSHNVPGYKYQMWATWTVPKSLPASSLVDWIVS
                     AVDRSPEMALKNIIINTHGSPGYLHIGSGVGVKDLNEFKRLRGKSIGYIWVIACEVSK
                     GVDLGNVCPVDEYNGPYFCTELAKASGTFVVASEELQSVDPGFTVFSVFVPSGYVDDY
                     EGAVYRYSPAGGRTRISANGSDAYD"
BASE COUNT         1220 a         2834 c         3013 g         1248 t
ORIGIN      
        1 gagcagcgcg cagcgtccgg cctcatcgtg tgccagtgcg ccgggatgct cggcgcagag
       61 caccgtgccg ggcgcgctcc gcgtggttcc gggcgcgacg acgctgcctg cgggcggaag
      121 gagggcggct tgcaccgcat cggggacggg gcggtccagg ctgtgccagc cacggaatgc
      181 atgcatgggg atcggcggat tcgtcggggg cagggagatt tccggtgacc ggcgtcagcg
      241 cgcagcgctc acggccactg gccgaccgag ccgagcttgt tgcgcaggcc cggactcgga
      301 tagcccatgt cgtaggcctt gtgtgcgttg tccttggctt cggcgtactg cttcagctcg
      361 aagtgcgcga ggccgatgtt gtaatagagt tcggcgctct tgaggccgag atcggtggcc
      421 tgcccgtagg catcgagcgc ctgctggtac ttgccgttcc agtgcaggta gacgccgagc
      481 agctggcgca cgatcggatc gcgcggcacg aagcgcatcg cacgctcgaa gtagcactcc
      541 ggtgtccccg gccacgcgct gctgtcgccg accacgcgcg gcggtttcgg caggttgccg
      601 ttgagcttgc ggtgcaggcg gatcagcagg aacagcgcgc gcggattgtt cgggatcgcg
      661 cgcagcgtgt agtcgatgct gcccatcgtc tcgaagtcgg gacggctcag ggattcgaaa
      721 gtacgcagat gtgcgccttc gacgagatcg atgatggtgc ctttggagcc gccgtaaccg
      781 ctctggcgta tcagcgggtc attgtaattg tatggcccga aagcattttc caggctgccg
      841 cagttctgct gcgcgaaccc ggccagcggc ccggccagca gcagcatgcc cagcagcagg
      901 gccggcagct cagcgctcgg tttcggcatg ttccagtgtc tcccggggcg aggccgtgcc
      961 gtggcgggca tcgcgcagcc agtgcacgag ccggcgcagc tgcggccagc ggatgtagcc
     1021 gcggtggtag atcgtgcgca ggtcatcggt caggcgccgg tgccagtcca tcgcccgtcc
     1081 gtagaactcg atcgtgcgca ggcccggatc ggcgaacagc tcggccagat aggcctgacg
     1141 catcagcatc gccggagagc tgccctggat ggattcgtcg taagtcgtct tcagcacgat
     1201 cagcgtgccg tcgtgctcga tgcagagatc ggtcgtgacc aggcggtcgt cgtagaaata
     1261 acggtagatg cggccctgac cacgacggca gaagcgttcg agcacctcgc ggtagaagtg
     1321 cccctgggcg ttgtccggcg acaccgccgt gccaccctcg gacttccagc cggcgctttc
     1381 cagccggccg taatcggcga tcgcagcggc gacaccggcc ggatcgcgga cgcattcgag
     1441 gcgcaggcac acgccctcgc gctcgaagcg gttgcgctgc ttgttgaggt tctggcgcag
     1501 gttcttgctg cgcccggccc accagctctc gaaaggcgca tcgatcgtga tgcgggcggt
     1561 ctcgatgtag tcgagctcgt ggtagcccgc ctgcggtccg ggccgcggat cgaactgcgg
     1621 gtcgagctgc agcaggtcga ggctcatcag cagggcgggc agcgcggtga tcagttcgcc
     1681 gatcagcgtg ccggcatcga ggccgggctg gcatacgagc ggcgtgatcg gcatctgcat
     1741 cggcacgaag ctgcgccagc ggccgagccc ctggcgttcg agcagcagca gggcctgggg
     1801 cgccgaggcc tcgccgcgga tcgcgagcac cggcgtgccg tgtccgggca gcgccagcag
     1861 cggttcgacg aagtcgggat ccagcagcgg gctgcggcgg cccgcctcgt tcagcgccgc
     1921 ccaggcagca cgctggcgtg cgaacccggt ggcggcgggc aggaaggtcc agctcatgcg
     1981 ggtactccgg tgccggcggc ggcgaccgcc gcgcgcacct gtgcggcaat ccagtcgatt
     2041 tcgtggctgc gcagcgcctg gtggcagggc agctgcagca gcgcgctccg gtagcggttg
     2101 gcgaccgggc aggcggtgag tgcggcctcc ggcatgtcct cccagcgcag caccggcacc
     2161 cgggcctgct tcagcgccgg aaacacctgc gccggagcat cgaccagcag cggcagcacg
     2221 tagggcacgg tctcgtccgg cagctccggg cgcagcggcc ggcagccggg caggccgctg
     2281 agcgccgcgg caaggcgccg gtagttggcg cgacgcgcct gcgcgatccg ggccgaggcc
     2341 cggcgcccgc gctgcatgcc gaggcgcgcg gcccggctca tgccggtctc cagccattcg
     2401 agtgcgagtt cggccccgcc gcccgacgag gaagggccga tgaccggcgc cgcggcgccg
     2461 tcggtgcgac cgcgccggcg cttgagcgcc gtccacagcg tgttcttcag ccacagcagg
     2521 ctgccgagca gcgacagccg gccgtacagc gtcgcgcgct ccagcggatc gagcacggcc
     2581 ttgatctgga aggcggcatc gccggtgcgc ggacgcacgg cttcgaggga atggcgggcc
     2641 gagatcaggc agccgccgtc gaagaccggg aagaacttca ccgggcttgc gatcgcgtag
     2701 tcgccgaagc tgccgagcgg ccgtccctgc cactggccga agaacgcgtg tgcgcagtcc
     2761 tcgatcagcg ctgcatcgtg cgcatcgcag agcgcgcgca cgcggccgat gtcctgcgcg
     2821 aagccgaaga aatgcgtggc gatcacggcc tttgcgccgg cggcgaggtg tgcggccatg
     2881 tcctcgaaat cgatcgaggt gtccgggtgc aggcggtaga acaccggctc ggcaccgagc
     2941 cactggatcg gatacagcat cgagatgcag tgataggccg gcagcagcac gcgatcaccg
     3001 gggccgatgc cgagcaggcg cagcgcgtgg cattcgccga gccggccggc ggtcagcagc
     3061 cgcacgtcgc cggcatcgag cacggacggc accgggtgcg tctcgagcgg cggccgggca
     3121 tcgaggttca ggatcggcgc gacgggcacg acgggcagat gcgtatgggg catgagcggg
     3181 ctgggcgggt ccgtgcgcgg aggtgtcggg gcaggggcgc gcgcacgata gcacagccgc
     3241 cgcgcctctc cggcggggcg gcggtatccg ggcggtgaca gccggttaca acggcgcagc
     3301 gcggcacccg gggatcaggt gccgctcggc gggggctcag gcgtcctcgg cacgcggccg
     3361 gtactgggcg atgagttcct tctgcacggc cggcggcacg gcctcgtagc gtgccagctc
     3421 cagcgtgtag ccgccgctgc cgccggtcat cgccttcagc cgcgccggat agtcgtcgag
     3481 ttcggcgagc ggtgcctgcg cgaccacggc cacgcgcccg gcgttgagcg tgcgcgtgcc
     3541 gaggatgcgc ccgcgccggc cggagagatc gccggtgacg ctgcccatcg cgtcctgcgg
     3601 aatctgcacg tcgagatcga cgatcggttc gagcacgacc ggacgcgcct cgcgtatcgc
     3661 cgcgaggaag gcctcgcgcc cggcggtcgc gaacgcgacc tccttggaat cgaccggatg
     3721 atgcttgccg tcggtgacga cgacgcgcac gtcgacgacc ggatagccgg cgatcgcgcc
     3781 gctcgcgcag gcctcgcgca cgcccttctc gaccgccggg atgaactggc cggggatggc
     3841 gccgccgacg acttcggagg cgaactcgaa gccggcgccg cgttcgagcg gctcgacgcg
     3901 caggtggacc tcgccgaact ggccggcgcc gccggtctgc ttcttgtggc ggtaatggcc
     3961 ctcggcgcgc gcctgcagcg tctcgcggta ggcgatgcgc ggcgggcggg tgtcgacctc
     4021 cagccggtag cgctcgcgca tcttgtcgag cgtcgagcgc aggtgctggt cggagagccc
     4081 gcgcagcacg gtctcgcggg tcgcggcgtt ggcttcgagc gtcaggcagg ggtcttcttc
     4141 gagcagccgg tgcagcacgt cggcgaggcg gttctcgtcg ttgtgcgtgc gcgcggcgag
     4201 cgcgatgccg gcgaccggca tcgggaagcg cgacggcgcg aggtgcaggt aatcctcgtc
     4261 atgcgagtcg tgcaggatgg cgtcgcggtg gatgtcgtcg atcttggcga cggcgcggat
     4321 gtcgccgggg atgccgttcg ggacttccgg gtgatcgcgt ccgaacagcg tgaacaggtg
     4381 cccgaccttg aagggcttgc ggccgtcgcc gatgtagagc tggctgtcct tcgtgaccgt
     4441 gccctgatgg atgcggaaca gcgcgagctt gccgacgaag gggtcgatca gcaccttgaa
     4501 cacgtgcgcg acgacgtgct tcgccggatc gggctcgacc gcgtacggca cggccggctc
     4561 gtcgccgcgc tgcagcaggc aggccggcgg attgccttcg cccgggttcg gcgcgagccg
     4621 ggtcaggatg tcgagcagct ccgggatgcc ggcaccggtg cgcgccgaca cgaagcagac
     4681 cgggatcagg tggccttcgc gcagcgcctg ttcgaagggc gcgtgcagct gcgccgggtc
     4741 gagttcctcg ccctgttcga ggtaggtcgc catcagggct tcatcgacct cgaccacctg
     4801 gtcgatcagc gcctggtgcg cggcggcgac cgacgggaaa tcggcctcgc cggccgggtt
     4861 gaagaagcag tcgacgaccg caccggcgcc ttgcgccggc aggttcagcg gcaggcactc
     4921 gcggccgaag gtttcctgca gctgcgcgag cagtcccggc aggtcgacgc cgtcggcgtc
     4981 gatcttgttg acgacgatca gccggcagag gccgcgctcg gcggcgcgcg ccatcatgcg
     5041 ctgcgtgacc ggctcgatgc cggcctgggc gttgacgacg atcgccgcgg tctcgaccgc
     5101 cggcagcacg gcgatcgcct gcccgaggaa gtccggcgca ccgggcgtgt cgagcaggtt
     5161 gacgtggcag ccgtcgcgat cgaaggacac cagcgccgcg tcgagcgagc ggccctgggc
     5221 cttttccagc ggatcggaat cgcagaccgt cgtgcccttc tcgacgctgc cggcgacggc
     5281 gatgcgcccg gccgccacga gcagcgcttc ggcgagcgag gtcttgccgg cgccggactg
     5341 gccgagcagg gcgaggttgc ggatgtcgcg ggtcgtgtaa gccatagttc ttgtctccgc
     5401 ctcgggtcgt gatcgcagca atgccattcg gtccagctta ggcagcgccg ccccccgatt
     5461 cgatgccgtg tgtcaatgcg ccactgcgcc cagcgcccgg cggtaggtct cgaacgcggc
     5521 actgccgaag cagcagaaca cgatgcgctg cggctgcgca tgggcttcgg cgtgcgcgcg
     5581 ggcactggcg acggcgatcg cggtggcctg atcgagcgga tagccgtaga tgccgcagct
     5641 gatcgccgga aacgcgatcg aggccgcacc gatcccgacg gcgaggcgca ggctctcgcg
     5701 gtagcagctc gcgagcagcg ccgcctcgtt ctccgtgccg ccgcgccaca ccggcccgac
     5761 cgtgtggatc acccagtgcg ccggcagccg gtagccggcg ctcacgcgcg cctcgccggt
     5821 cgggcagccg ccgagcatgc ggcactcctc gtagagttgc ggcccggcgg cgcggtggat
     5881 cgcgccatcg acgccgccgc cgccgagcag cgaggcattc gcagcgttga cgatggcgtc
     5941 gaccgcgagc ttcgtgatgt cggcctcgat gacttcgaat cgcgtgttca tgacgacctc
     6001 ccgctgagct gagctgcgcg accggggccg gtgcgcagac ggcgagcgta ggccgcccgc
     6061 cgccggtctg ccggtatcgt cggcgcgccg ctgcccgcat aatccgcgcc cttcgcggct
     6121 cccgcgccgc gcctcgcccg ttttccggag agattccgat gctcgacccc aggctgctcc
     6181 gttccgaact cgacgacacc gcccgccgtc tggcgcggcg cggctacgtg ctcgacaccg
     6241 gtcgcatccg cggcctcgaa gaacagcgca aggccacgca ggtgcgcgcg caggagctgc
     6301 aggccgtgcg caacagccgc tcgaaggcga tcggcaaggc acgcggggcc ggtgaggaca
     6361 tcgccccgct gctcgccgag gttgcgagcc tcggcgacga gctgaaggcc gccgagacgg
     6421 cgctcgccgc gctgcaggcc gagctcgacg agatctactc cggcatcccg aacctgcccg
     6481 atgactcggt gccggacggg cgcgacgagc aggacaacgt cgagctgcgc cgctggggca
     6541 cgccgcggca gttcgactgg acgccgctcg atcacgccga cctgctgccg ggctttctcg
     6601 atttcgagtc ggccgccaag ctcaccggcg cgcgcttcag cgtgctgcac gggccgctcg
     6661 cgcgcctgca ccgggcgctg atccagttca tgctcgacct gcacaccgtc gagcacggct
     6721 acagcgaggt ctacgtcccg tatctggtca acgccgacac gctgcgcggc accggccagc
     6781 tgccgaagtt cgaggcggat ctcttcagac tgagtggcga gttcccgtac tacctgatcc
     6841 cgaccgccga ggtgccggtc accaacctcg cgcgcggcga gatcctcgaa gccgcgacgc
     6901 tgccgcgcca gtacgccgcg cacacgccgt gcttccgctc cgaggccggt tcctacggca
     6961 aggacgtgcg cggcctgatc cgccagcacc agttcgagaa ggtcgaactc gtgcatctgg
     7021 tgcgccccga cgacagcttc gccgcgctcg aacgcctgac cgcgcacgcc gagaccgtgc
     7081 tgcagcgtct ggagctgccg tatcgcgtca tcgtgctgtg caccggcgac atgggcttcg
     7141 gcgccgtgcg cacgcacgat atcgaggtct ggctgccggg gcagggcaag taccgcgaga
     7201 tttcctcgtg ctcgaacatg ggcgacttcc aggcccggcg catgcaggca cgctggcgca
     7261 accccgccac cggcaagccc gagctgctgc acacgctgaa cggctccggc ctcgcggtcg
     7321 gacgcacgct ggtggcgatc ctcgagaact accagcaggc cgacggctcg gtgacgatgc
     7381 cggcggcgct gaagccctat ctcggcggcc tggagtcgat ccggccgaag cgctgaagcc
     7441 gaaacggcgg gccgcatccg cgccgctcgc cggggtggat gcggccctct cgtcgttccc
     7501 ggcggacccc ggcggccgcc ggcgaaccgc agacccggcg cagcggtgcc ggccgctgca
     7561 gtcacgatgc tccgctgccg caccgctgca acacgccctg cacccgcagg ccctgatctg
     7621 acaggatttt ccgcaagtcg gccccggcgc acctagattg cgcttaccgc gctgcccggc
     7681 gcgggccacc gcggagtgat cgtcatgatt tcgatgaagc agccttcgct cgtgctgcat
     7741 tcgcacaacg tgcccggtta caagtaccag atgtgggcga cctggacggt gccgaagagc
     7801 ctgccggcga gttcgctggt cgactggatc gtctcggccg tcgaccgctc gccggagatg
     7861 gcgctgaaga acatcatcat caacacgcac ggctcgcccg gctacctgca catcggcagc
     7921 ggcgtcggcg tgaaggatct caacgaattc aagcgcctgc gcggcaagtc gatcggctac
     7981 atctgggtga tcgcctgcga ggtcagcaag ggcgtcgatc tcggcaacgt ctgcccggtg
     8041 gacgagtaca acggcccgta cttctgcacc gagctcgcca aggccagcgg caccttcgtc
     8101 gtcgcctccg aggaactgca gtcggtcgat cccggcttca cggtgttctc ggtgttcgtg
     8161 ccctcgggct acgtcgatga ctacgagggg gcggtctatc gctacagccc ggccggcggg
     8221 cgcacgcgca tctcggccaa tggctccgat gcgtatgact gattcccctg tctgatttcc
     8281 tccgccggcc ggcggaggaa atcagacagg ggaat
//
LOCUS       DAOQIE010000171         8219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4454_length_8219_cov_8.748163, whole genome shotgun sequence.
ACCESSION   DAOQIE010000171 DAOQIE010000000
VERSION     DAOQIE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4454_length_8219_cov_8.748163"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="tssL"
                     /locus_tag="PK144_11295"
     CDS             <1..471
                     /gene="tssL"
                     /locus_tag="PK144_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HNF66359.1"
                     /translation="VAPAPPPPAPTPPRLKGFLEDEIRQGLVAVDDRDDRSVITIRGD
                     GLFASGAATVKDAYLPVLGRIATELKRVPGEVLVTGHSDNKPIATLRFPSNWKLSQAR
                     AESVLELLARDTGQPLRFTAEGKADTEPVAPNDTNDGRAQNRRVEITLFAPGVR"
     gene            502..4047
                     /gene="tssM"
                     /locus_tag="PK144_11300"
     CDS             502..4047
                     /gene="tssM"
                     /locus_tag="PK144_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HNF66360.1"
                     /translation="MKAFIKNWLIPLLALGALAALIWFVGPLIAIGGWVPFESETVRW
                     IAIGVVALLWLIWRIVRLISAKKHEREMLAGLVEAPPDPAAVESAEEIAALRRRLDEA
                     VGVLKKARLGASGGERQTLYQLPWYILIGPPGSGKTTALIHSGLNFPLAEHFGKDAIR
                     GVGGTRNCDWWFTDEAVLLDTAGRYTTQDSHAAVDKAAWLGFLDLLKQHRRRRPISGA
                     LIAISVAELMQSSEAERNAHARAIRARIQELHERLGIRFPIYVLLTKSDLIAGFTEFF
                     DDLGKDERGQVWGMTFPLDETGNGEPPLARFATEFAALEQRLSARQTERLQGERDPGR
                     RALIYAFPKQFEALRDLLDGFLNEAFRPNAYEQRPLLRGVYFTSGTQEGTPIDRLMAG
                     MAQTFRLARTQLGSLGNSGRSYFLTRLFRETVFPEQGLAGVNLKLERRRAWLQAGAWA
                     AALLITLGMGALWLLSWQGNRTLITDTDAQVTDVRKLLEKVSPDQREALAVLPLLDAV
                     RTLPGGYAERDEGTPWRLGFGLYQGDMLGSEATAVYRRMLHGALLPRLILRLEQQIRA
                     NQANPDILFVALKTYLMLENPDHYEPDAIKAWIALDWDNNLPRDVTREQRQHLREHLD
                     ALFEKRPLPLPLALDRQLIGQTQAILVRVPLPVRAYDRMKRFVAQDNKAYPDFRIDTA
                     AGRDAGLVLRRASGKPLGEGVPGFYTYRGYTEGFGKAGTELVARLAEESWVLGDVGRI
                     APQDLPQLIEDVRRLYFADYIKVWDALIADVGVVPLGNTQQAIDILRVLSGPGSPLKL
                     WLEAVARETNLARKEGLAAAGDKAADSAVDKLKQQLASLIGSTGATPPPAVTAAQELD
                     PVTRHFADLAAQVRKDGSNPAPIDATLAAFNDLYVKLNTQAGASGDALIKDAAGQTAA
                     VVRGLKLDADRAPPAVGAVMKAVADQADGLTMGGVRNQINQLWQAEVADFYRQTLAGR
                     YPLARSPRDAALADFGEFFAPDGRIDKFFKNYLARYVDQSTKPWRWQPNATDKLGISA
                     GVLVQFQNAATIRDAYFRAGTAGPGVRFDLEPVSMDAEIAQIIVDLDGQLLTYAHGPT
                     RATAMQWPTPNGAGNLQISITPPGNSGRTGLSYDGPWALFRMLDQQSVRGSGEVYTVT
                     INVDGRSASFRLRANSAFNPFRLPALGAFSCPDRL"
     gene            4048..4761
                     /gene="tagF"
                     /locus_tag="PK144_11305"
     CDS             4048..4761
                     /gene="tagF"
                     /locus_tag="PK144_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010447864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HNF66361.1"
                     /translation="MAACGFYGKLASRGDFVSRRLPREFVEPWDGWLQAGLSASREQL
                     GDGWLDCYLTSPLWRFVLAPGCCGPQVTVAGVLMPSVDRVGRYFPLTLACSLDGELTP
                     DALARDAGDWYAQAEALALATLEETLDLDGWDAAVAALGAPAAAVGLALPGTPMLGHF
                     EDLADAGSMAGRLAQQRLRPDWRGSSLWWTLGSERIEPCVILAEGLPPAGTLGSFFDG
                     DFARAGYAAGDTAGSTHDQ"
     gene            4835..5605
                     /locus_tag="PK144_11310"
     CDS             4835..5605
                     /locus_tag="PK144_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNF66362.1"
                     /translation="MLGDHPLRFESSAVTHVGMVRKVNEDAVLDQSGRGMWAVADGMG
                     GHAAGDFASRTIVQTLSRLPPAPTISVYVRSVQTSLQHINELLRQEAARRERETIGAT
                     VAVLVAAADELACLWAGDSRLYRFRRPRLEQLTRDHSYVQELVDAGALSPEEAQRHPM
                     GNVVTRAVGAHETLEVDAHPVITRPGDVLLLCTDGLSKVVSDDEIAQVLATRRPAEAT
                     RELVNLALLRGAPDNVSLVVVRVDADTGTVTQPVTPVV"
     gene            complement(5619..6068)
                     /locus_tag="PK144_11315"
     CDS             complement(5619..6068)
                     /locus_tag="PK144_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66363.1"
                     /translation="MLVPALALALLGGCTIPLPAWLGGGGGGPPSPAKPAAGVVAPLS
                     IDDAGLLATRERRLELGLRLSSRSGETQWVSVRFQTPDGQDCVSLKELPAQGRLLFTC
                     AQARLVAELDYPVTIESWRDAGLQSAFAPIRTTLRFTRNDLVAAGVR"
     gene            complement(6192..7547)
                     /gene="hslU"
                     /locus_tag="PK144_11320"
     CDS             complement(6192..7547)
                     /gene="hslU"
                     /locus_tag="PK144_11320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNF66364.1"
                     /translation="MTEMTPREIVQELDRHIVGQAAAKRAVAIALRNRWRRMQITGPL
                     RAEITPKNILMIGPTGVGKTEIARRLAALARAPFIKVEATKFTEVGYVGRDVETIIRD
                     LVDAAMKLAREDATRKVRVRAEENAIERVLDALLPRPRPAATGGFFSNEPAEPPPSEN
                     TATRQKMREKLLRGELDEREIEIELAASAPGVEIMAPPGMEEMTNQLQSLFQNLAQNR
                     TRARKLRIREALKLLQEEEAARLVNPDDLKHQALEAVEQNGIVFIDEIDKVCRRGEYS
                     GADVSREGVQRDLLPLVEGCTVSTKYGAVKTDHILFIASGAFHLAKPSDLVPELQGRL
                     PIRVELDALSAQDFVRILTEPHASLTEQYKALLATEGFGVTFAPDAVQRLAEIAHSVN
                     ERTENIGARRLHTVLERLLEDVSFEAADRDGQTLLIDAAYVDAHLGQLVQDEDLSRFI
                     L"
     gene            complement(7567..8100)
                     /gene="hslV"
                     /locus_tag="PK144_11325"
     CDS             complement(7567..8100)
                     /gene="hslV"
                     /locus_tag="PK144_11325"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNF66365.1"
                     /translation="MEQFRGTTILAVRRNGRIAIGGDGQVTLGNTVMKGNARKVRRLY
                     QGKVVAGFAGGTADAFTLFERFEGKLEKHGGNLTRAAVELAKDWRTDRMLRRLEALMI
                     VADASAMLVISGNGDVIDPEHDLAAIGSGGAFAQAAARALLAHSELDARGVVEAALNI
                     AAEICIYTNPHLTIEEL"
BASE COUNT         1220 a         2967 c         2841 g         1191 t
ORIGIN      
        1 gtcgcaccgg ccccgccgcc gcccgcgccg acaccgccgc ggctcaaggg ctttctcgag
       61 gacgagatcc gccagggact ggtcgccgtc gacgaccgcg acgaccgcag cgtcatcacg
      121 atccgcggcg acggcctgtt cgcctcgggc gcggcgacgg tcaaggacgc ctatctgccg
      181 gtgctcgggc gcatcgccac cgaactgaag cgcgtgcccg gcgaggtgct ggtcaccggc
      241 cacagcgaca acaagccgat cgcgacgctg cgcttcccgt cgaactggaa gctgtcgcag
      301 gcgcgcgccg agtcggtgct cgaactgctc gcccgcgaca ccggccagcc gctgcgcttc
      361 accgccgaag gcaaggccga caccgagccg gtcgccccga acgacaccaa tgacggtcgg
      421 gcgcagaacc ggcgcgtcga gatcaccctg ttcgctcccg gcgtgcgctg aaccggcact
      481 gcgtccggga cgaggaccgt catgaaggct ttcatcaaga actggctgat cccgctgctc
      541 gcgctcggtg cactcgcggc gctgatctgg ttcgtcggcc cgctgatcgc gatcggcggc
      601 tgggtgccgt tcgaatccga gacggtgcgc tggatcgcga tcggtgtggt cgcgctgctg
      661 tggctgatct ggcgcatcgt gcggctgatc agcgcgaaga agcacgagcg cgagatgctc
      721 gccggtctgg tcgaggcgcc gcccgatccg gcggcggtcg aatcggccga ggagatcgcc
      781 gcgctgcgca ggcgcctcga cgaggccgtc ggcgtgctga agaaggcgcg cctcggcgcg
      841 agcggcggcg agcgccagac gctctaccag ctgccgtggt acatcctgat cggcccgccg
      901 ggctcgggca agacgacggc gctgatccac agcggcctga acttcccgct cgccgagcac
      961 ttcggcaagg acgcgatccg cggcgtcggc ggcacccgca actgcgactg gtggttcacc
     1021 gacgaggccg tgctgctcga caccgccggc cgctacacca cgcaggacag ccacgccgcg
     1081 gtcgacaagg ccgcgtggct cggcttcctc gacctgctga agcagcaccg ccgccgccgc
     1141 ccgatcagcg gcgcgctgat cgcgatctcg gtcgccgagc tgatgcagtc ctccgaggcc
     1201 gagcgcaacg cccatgcgcg cgcgatccgc gcccgcatcc aggagctgca cgagcgcctc
     1261 ggcatccgct tcccgatcta cgtgctgctg accaagagcg acctgatcgc cggcttcacc
     1321 gagttcttcg atgatctcgg caaggacgaa cgcggtcagg tctggggcat gaccttcccg
     1381 ctcgacgaga ccggcaacgg cgagccgccg ctcgcgcgct tcgccaccga gttcgcagcg
     1441 ctcgaacagc gcctgtcggc gcgccagacc gagcgcctgc agggcgagcg cgatccgggg
     1501 cgccgggcgc tgatctacgc cttcccgaag cagttcgagg cgctgcgcga cctgctcgac
     1561 ggcttcctga acgaggcctt ccgcccgaac gcctacgagc agcgcccgct gctgcgcggc
     1621 gtgtacttca ccagcggcac gcaggaaggc acgccgatcg accggctgat ggccggcatg
     1681 gcacagacct tccggctggc gcgcacgcag ctcggcagcc tcggcaacag cggccgcagc
     1741 tacttcctga cccggctgtt ccgcgagacc gtgttcccgg agcagggcct cgccggcgtc
     1801 aacctgaagc tcgaacgccg gcgcgcctgg ctgcaggccg gtgcctgggc cgcagcgctg
     1861 ctgatcacgc tcggcatggg cgcgctgtgg ctgctgagct ggcagggcaa ccggacgctg
     1921 atcaccgata ccgacgcgca ggtcaccgac gtgcgcaagc tgctggagaa ggtctctccg
     1981 gaccagcgcg aggcgctggc ggtgctgccg ctgctcgatg ccgtgcgcac gctgcccggc
     2041 ggctacgccg agcgggacga aggcacgccg tggcggctcg gcttcgggct gtaccagggc
     2101 gacatgctcg gcagcgaggc cacggccgtg taccggcgca tgctgcacgg cgcactgctg
     2161 ccgcggctga tcctgcgcct ggagcagcag atccgcgcca atcaggccaa tccggacatc
     2221 ctgttcgtgg cgctgaagac ctacctgatg ctcgaaaacc cggatcacta cgaacccgat
     2281 gcgatcaagg cctggatcgc gctcgactgg gacaacaacc tgccgcgcga cgtgacgcgc
     2341 gagcagcgcc agcacctgcg cgagcacctc gacgcgctgt tcgagaagcg cccgctgccg
     2401 ctgccgctcg cgctcgaccg ccagctgatc ggccagacgc aggcgatcct cgtgcgcgtg
     2461 ccgctgccgg tgcgcgccta cgaccgcatg aagcgcttcg tcgcgcagga caacaaggcc
     2521 tacccggact tccgcatcga caccgccgcc ggccgcgatg ccgggctggt gctgcgccgc
     2581 gcgagcggca agccgctcgg cgagggcgtg ccgggcttct acacctaccg cggctacacc
     2641 gagggcttcg gcaaggccgg caccgagctg gtcgcgcgcc tcgccgagga gagctgggtg
     2701 ctcggcgatg tcgggcgcat cgcaccgcag gatctgccgc agctgatcga ggacgtgcgc
     2761 cggctgtact tcgccgacta catcaaggtc tgggacgcgc tgatcgccga tgtcggcgtc
     2821 gtgccgctcg gcaacaccca gcaggcgatc gacatcctgc gcgtgctctc cggccccggc
     2881 tcgccgctga agctctggct cgaagccgtc gcccgcgaga ccaacctcgc gcgcaaggaa
     2941 ggtctggccg cggccggcga caaggctgcc gacagcgccg tcgacaagct caagcagcag
     3001 ctcgcgagcc tgatcggctc gaccggcgcc accccgccgc cggcggtcac ggccgcgcag
     3061 gagcttgatc cggtcaccag gcacttcgcc gacctcgccg cacaggtcag gaaggacggc
     3121 agcaatccgg cgccgatcga cgcgacgctc gccgccttca acgacctgta cgtgaagctc
     3181 aacacccagg ccggtgccag cggcgatgcg ctgatcaagg acgccgccgg ccagaccgcg
     3241 gcggtcgtac gcgggctcaa gctcgacgcc gatcgcgcgc caccggccgt cggcgcggtc
     3301 atgaaggccg tcgccgatca ggccgacggc ctgaccatgg gtggcgtgcg caaccagatc
     3361 aaccagctct ggcaggccga ggtcgccgac ttctaccgcc agacgctggc cgggcgctac
     3421 ccgctggcgc gcagcccgcg cgatgccgcg ctcgccgact tcggcgagtt cttcgctccg
     3481 gacggccgca tcgacaagtt cttcaagaac tatctcgcgc gctacgtcga ccagtcgacc
     3541 aagccctggc gctggcagcc gaacgccacc gacaagctcg gcatctcggc cggcgtgctc
     3601 gtgcagttcc agaacgccgc gaccatccgt gatgcctact tccgcgccgg caccgccggc
     3661 cccggcgtgc gcttcgacct cgaaccggtg tcgatggatg ccgagatcgc gcagatcatc
     3721 gtcgatctcg acggccagct gctgacctac gcgcacggcc cgacccgcgc cacggccatg
     3781 cagtggccga ccccgaacgg cgccggcaac ctgcagatca gcatcacgcc gcccggcaac
     3841 agcggccgca ccggcctgag ctacgacggg ccgtgggcgc tgttccggat gctcgaccag
     3901 cagtcggtgc gcggcagcgg cgaggtgtac acggtcacga tcaacgtcga tggccgcagc
     3961 gccagcttcc ggctgcgcgc caacagcgcc ttcaacccgt tccggctgcc ggcgctcggc
     4021 gccttcagct gcccggatcg gctgtagatg gccgcctgcg gtttttacgg caagctcgcg
     4081 agccgcggcg acttcgtgtc gcgccgcctg ccgcgcgagt tcgtcgaacc ctgggacggc
     4141 tggctgcagg cgggcctcag cgccagccgc gagcagctcg gcgacggctg gctggactgc
     4201 tacctgacca gcccgctgtg gcgcttcgtg ctggcgccgg gctgctgcgg accgcaggtc
     4261 acggttgccg gcgtgctgat gccgagcgtc gaccgggtcg ggcgctactt tccgctgacg
     4321 ctcgcctgca gcctcgatgg tgagctcacg cccgacgcgc tggcccgcga tgccggcgac
     4381 tggtacgcac aggccgaagc cctggcgctc gcgacgctgg aggaaacgct ggacctcgac
     4441 ggctgggatg ccgccgtcgc cgccctcggc gccccggccg ccgcggtcgg gctggcgctg
     4501 ccgggcacgc cgatgctcgg gcactttgaa gacctcgccg acgccggcag catggccggc
     4561 cggctcgcgc agcagcgcct gcgccccgac tggcgcggca gctcgctgtg gtggacgctg
     4621 ggctcggagc gcatcgagcc ctgcgtgatt ctggccgagg gcctgccgcc tgccggcacg
     4681 ctcggcagct tcttcgacgg ggacttcgcc cgggccggct atgccgcggg cgacaccgca
     4741 ggatccacgc atgatcaatg atgacaactt gttaccgtcc ggcgcgccgc tgccactacc
     4801 ttccagtcgc accgacgaca ccggggacag cgcgatgctc ggcgatcacc ccttgcgctt
     4861 cgaatcgagc gccgtcacgc acgtaggcat ggtccgcaag gtcaacgagg atgccgtgct
     4921 cgaccagtcg ggccgcggca tgtgggccgt ggccgatggc atgggcggtc acgcggccgg
     4981 cgatttcgcg tcccggacca tcgtgcagac gctgtcgcgc ctgccgccgg cgccgacgat
     5041 cagcgtctat gtccgctcgg tgcagaccag cctgcagcac atcaacgagc tgctgcgtca
     5101 ggaagccgca cgccgcgagc gcgagaccat cggcgcaacg gtcgcggtgc tggtcgccgc
     5161 cgctgacgaa ctggcctgtc tgtgggccgg tgacagccgc ctgtaccgct tccgccgtcc
     5221 gaggcttgaa cagctgacgc gcgatcacag ctacgtgcag gaactcgtcg atgccggcgc
     5281 cctgagcccg gaggaagccc agcgccaccc gatgggcaac gtcgtcacgc gcgccgtcgg
     5341 tgcccacgag acgctggagg tcgatgccca cccggtgatc acccgccccg gcgacgtgct
     5401 gctgctctgc accgacggcc tgagcaaggt cgtcagcgac gacgagatcg cgcaggtgct
     5461 cgccacccgc cggccggcgg aggccacccg tgaactcgtc aatctggcgc tgctgcgcgg
     5521 cgcgcccgac aacgtcagcc tcgtcgtcgt gcgcgtcgac gccgacaccg gcacggtcac
     5581 gcagccggtg acgccggtcg tctgagcacg cgcgcccgtc agcgcacgcc ggcggcgacc
     5641 agatcgttgc gcgtgaagcg cagcgtcgtg cggatcggcg cgaaggcgct ctgcaggccg
     5701 gcgtcacgcc agctctcgat cgtgaccggg taatcgagtt cggcgacgag ccgcgcctgc
     5761 gcacaggtga acagcagccg gccctgcgcc ggcagttcct tcaggctcac gcagtcctgg
     5821 ccgtcgggcg tctggaaacg caccgacacc cactgcgtct cgcccgaacg gctcgacagc
     5881 cgcagcccca gttcgagccg ccgctcgcgc gtcgcgagca ggccggcatc gtcgatcgac
     5941 agcggtgcca ccaccccggc cgccggcttc gccggtgacg gcggtccgcc gccaccgccg
     6001 ccgagccagg ccggcagcgg gatcgtgcag ccgccgagca gggcgagcgc gagggcgggg
     6061 acaagcaccg cacgggcgcg gacccggcgg acagggcaag ggacagtcgg gacgggcatc
     6121 ggtggcggca tccggaaacg gcagcgggcg cccactataa gcgcccgccg ggcttcggat
     6181 cgcgccatgc gtcagaggat gaagcggctg agatcctcgt cctgcaccag ctggccgagg
     6241 tgcgcatcga cgtaggccgc gtcgatgagc agcgtctggc cgtcgcggtc cgcggcttcg
     6301 aacgagacat cctccagcag ccgttcgagc acggtgtgca gccggcgcgc gccgatgttc
     6361 tcggtgcgct cgttgacgct gtgggcaatc tcggcgaggc gctgcacggc gtcgggcgcg
     6421 aaggtgacgc cgaagccctc ggtcgcgagc agggccttgt actgctcggt cagcgaggcg
     6481 tgcggctcgg tcaggatgcg cacgaagtcc tgcgcgctca gggcatcgag ttcgacgcgg
     6541 atcggcaggc ggccctgcag ctccggcacc agatccgagg gcttggccag atggaaggcg
     6601 cccgaggcga tgaacaggat gtggtcggtc ttcacggctc cgtacttggt cgacaccgtg
     6661 cagccttcga ccagcggcag caggtcacgc tgcacgcctt cgcgcgacac gtcggcgccg
     6721 ctgtattcgc cgcgccggca gaccttgtcg atctcgtcga tgaacacgat gccgttctgc
     6781 tcgacggcct ccagcgcctg atgcttgaga tcatcggggt tgacgagccg cgcggcttct
     6841 tcttcctgca gcagcttcag cgcctcgcgg atgcgcagct tgcgcgcgcg cgtgcggttc
     6901 tgcgcgaggt tctggaacag gctctgcagc tggttcgtca tttcctccat gccgggcggc
     6961 gccatgatct cgacgccggg ggcgctggcg gcgagctcga tctcgatctc gcgctcgtcg
     7021 agttcgccgc gcaggagctt ctcgcgcatc ttctggcgcg tggcggtgtt ctcggacggc
     7081 ggcggctcgg ccggttcgtt gctgaagaag ccgccggtcg ccgccgggcg cgggcgcggc
     7141 agcagcgcgt cgagcacgcg ctcgatggcg ttctcctcgg cgcgcacgcg caccttgcgc
     7201 gtcgcatcct cgcgcgcgag cttcatcgcc gcatcgacga gatcgcggat gatggtttcc
     7261 acgtcgcgcc cgacgtagcc gacctcggtg aacttggtgg cctcgacctt gatgaacggg
     7321 gcacgcgcaa gggcggcgag gcggcgggcg atctcggtct tgccgacacc ggtcgggccg
     7381 atcatcagga tgttcttcgg cgtgatctcg gcgcgcagcg gaccggtgat ctgcatgcgc
     7441 cgccagcggt tgcgcagcgc gatcgcgacg gcacgcttgg cggccgcctg gccgacgatg
     7501 tggcggtcca gttcctggac gatttctcgg ggcgtcattt cggtcatgtc ggggatccgg
     7561 gccggctcac agctcttcga tcgtcaggtg cgggttcgtg tagatgcaga tctcggcggc
     7621 gatgttcagc gccgcctcga cgacgccgcg cgcatcgagt tccgagtgcg cgagcagcgc
     7681 ccgcgccgcc gcctgcgcga aggccccgcc ggagccgatc gccgccagat cgtgctcggg
     7741 atcgatgaca tcaccgttgc cggaaatcac cagcatcgcg ctggcatcgg cgacgatcat
     7801 cagcgcttcc agccggcgca gcatgcgatc ggtacgccag tccttggcga gttcgacggc
     7861 ggcccgcgtc aggttgccgc cgtgcttttc gagcttgccc tcgaagcgtt cgaacagcgt
     7921 gaaggcatcg gccgtaccgc cggcgaaacc ggccaccacc ttgccctgat agagccggcg
     7981 caccttgcgg gcgttgccct tcatgaccgt gttgccgagc gtgacctgac cgtcaccgcc
     8041 gatggcgatg cggccgttgc ggcgcaccgc gaggatcgtg gtgccgcgaa actgttccat
     8101 cgaagtggcc tccggagctg ccctgatgac gaagcggctg ccgcgcgggt gaggcggcag
     8161 cacacgaacc gtcgaaatgc ggcgctgcgg cagcggtttc aagagcgccc ggaaagccg
//
LOCUS       DAOQIE010000172         8195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4472_length_8195_cov_6.615111, whole genome shotgun sequence.
ACCESSION   DAOQIE010000172 DAOQIE010000000
VERSION     DAOQIE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_8195_cov_6.615111"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PK144_11330"
     CDS             complement(<1..1449)
                     /locus_tag="PK144_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNF66366.1"
                     /translation="MAERIRSLSRPFRRSRRVAARVAALAVALALAGCMVGPDYQRPA
                     LELPAGTAGVPRIADDWWRSFNDPVLDGFEAEALAHNLDLAEAVARIDEARASLRGAR
                     GQQLPDATVGVNQSRSRISGVGSTPLPAGTPLTSSDRRVSLDLSWEIDLWGRLARGTE
                     AARAELAASEAARDGVRLSLTATVARSLFEARALAAQLDITRQTVTSRRQTAGLQQKR
                     FRAGSVSELEASQAEAEAADAAARLPDLEQQLAAARNALAVLLGRTPQALVERPVGDL
                     GGEEKLPPLPSVPAALPSDLLERRPDVRSAEQQLVAANARIGAAKADYFPRLTLTGSY
                     GAESQALSDLFTGPANTWSLAAGLVQPLFTGGRIAANVEGAEARTAQAEIAYRRTVQA
                     AFRETLDALVAHRSASERLRAREEREAALARSLKLANRRYEAGYSSWLDVLDAERGLF
                     QARLDRVEARRVRLDAAIGIIEAMGGGWTVPGK"
     gene            complement(1442..4612)
                     /locus_tag="PK144_11335"
     CDS             complement(1442..4612)
                     /locus_tag="PK144_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNF66367.1"
                     /translation="MNSRYFIERPILASVLSIVIVLAGLASMRALPIAQYPDIVPPEV
                     QVTAFYPGASAEVVAATVAAPLEQAINGVDDMLYVRSNSSAGALTIAASFAIGTDPDQ
                     ATINVNNRVQSALQSLPEEVRRQGVKAEKQSSNLLQIVMLDSPSKRFDTVFISNYALV
                     NVVDELRRINGVGNAMIFSARDYSMRIWIRPDRLASLGLTPSDIAAAVREQNSQFAAG
                     KFGQAPMGTPQEFTYTVTTQGRLATPEEFGAIVLRAEADGTLLRLRDVARIELGAADY
                     GMVAKRSGNETVAIGIFLAPGANALAVAGAVDARMKELARHFPVGLQWTAPYDTTRFV
                     EISIREVVKTLAEAMGLVFLVVFLFLQSARATIIPFLAVPVSLIGTFAGMYLLGFSIN
                     TLTLFGMVLAIGIVVDDAIVVLENVERIMRTQRLPPREATIQAMQEVSGPVIAIVLVL
                     CAVFVPVGFAGGLAGQMYKQFAITIAVSVVLSGFVALTLTPALCATLLKPVAHEPGRF
                     FRAFNRGFEHVTNGYSAGVRFFLKRALLGVALFAGMLVLTWGLFQRVPGSLVPDEDQG
                     YVMTMAILPPGASLERTIAVNDRINEFALANPAVDNVIALSGLDLLSFSNVPFYGAAF
                     IPLKHWEDRTAPGQDAQSLVGQFFMQGAQIRDAIVLAFNPPPISGMSTTGGFEAYLQS
                     RGGGGSKALAGEVQKFLAAAGQRPELAGLNTIFNASVPQIDIRLDREKARALGVPVNT
                     VFETMQATFGALYVNDFNKLGRTFKVQVQSEAEYREKPDDLKYVYVRSTDGRMIPLTA
                     LVEVVRSAGAEVVDRYNVFPAAKIVGGPAPGYSSGQALAAMEQVAAQTLGEDYSLVWT
                     GSAYQERLTGGTSTLVFGFGLVMVFLILAALYERWTLPVAVVLAVPFALFGALAAVWV
                     RGMANDVYFQIGLVTLIGLAAKNAILIVEFALLKAQDGVPVAQAALEAARLRFRPIIM
                     TSLAFVLGVVPLALSTGAGAASRHSIGTGVIGGMLAATFLATFFVPLFFRLVVRHAPK
                     PHHHGAAADPHDPSDPSAKAPEPHHG"
     gene            complement(4697..5926)
                     /locus_tag="PK144_11340"
     CDS             complement(4697..5926)
                     /locus_tag="PK144_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF66368.1"
                     /translation="MIRLFTRPLSRLFRRPDLILMRLVTVWIFAGALIVLVAGCDSRA
                     NNDSANHAMPPPEVARSVVRAADVELPLEYVAQVGGSREVQVRARVEGILQKRTYTEG
                     KPVKAGETLFLIDPAPFQAALDRARADLAEQEVRLAQADKELKRVQPLFADRAVSQKD
                     RDAAQAERDQASAAVKSARAAVRTAEIDLGYTRVTAPIAGITSREVRSEGSLVRPGDA
                     SGALTTLNQVDPAYVRFTYAENEALMIRKAIAEGRLRMPAGGPQVEVVLADGSVHPQA
                     GRIDFTDTLVDPQTGTVGVRATVANPDATLLPGQFVRVRLKGVTRPGAIVVPQRAVMQ
                     GPDGQFVWVIGDGDKVEFRPVVTGASIGKGWIVEEGLKGGERVATDNLLKLAPGALVV
                     EPKPAPAAAAGGHAPQG"
     gene            6111..6728
                     /locus_tag="PK144_11345"
     CDS             6111..6728
                     /locus_tag="PK144_11345"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNF66369.1"
                     /translation="MVRRTKAEAEQTRSLILDTAERLFFTKGVSRTTLAEIATAAGLT
                     RGAIYWHFANKLELFNAMHERVALPFTEAMEKLLHAEDPLAALRERCVLDLQDIAACP
                     QLQQVLAVLPHKCEYVDELGELCLRFDQQNKCFVEHSETVLARAHALGQLRDGVEPRR
                     AAIALHNYMLGTISDWLFARDLRYRLADEAGPLVDLFFAGLERPA"
     gene            complement(6832..8088)
                     /locus_tag="PK144_11350"
     CDS             complement(6832..8088)
                     /locus_tag="PK144_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF66370.1"
                     /translation="MRPFLLPSLFAFVASLPFAALVHADEVVIDPIQREHDRLQALTT
                     PEPPTPGSWHMGAGPKAAEPLRARLAEWGYDVPRGDDNALAAALVDFQRINGLKETGS
                     LDKSTVAKLSMSREDRLVRLRYTLAAETAQPIDRTVPRLVIVNIPSYKLRAFESGQLA
                     LSSNVVVGRPKRPTPELSVFMKAVKYNPTWTPPPTIIKEDLIPLFGRDPSAVTRKGLV
                     VLDRGGRQIPPDAIGPISAAGFHAAGYRMYQPAGDSNALGKLKFEITDTPSVYLHDTN
                     HRNDFGKPVRALSSGCVRVHQYRPLAAWLMGLSEDEIQKRINRRRTVVEPSPQIPIYL
                     VYRPAEAIDGHVVYYSDIYGKLNGPLPKPKPIPVIEPAPAPEATPAAPSADPSVPPVA
                     PVTPAAVPVATTPAVATASEPAPATR"
BASE COUNT         1262 a         2813 c         2943 g         1177 t
ORIGIN      
        1 cttccccggc accgtccacc ccccgcccat cgcctcgatg atcccgatcg ccgcatcgag
       61 gcgtacgcgc cgggcctcga cgcggtcgag cctggcctgg aacaggccgc gttcggcgtc
      121 gagcacgtcg agccagctcg aatagccggc ttcgtagcgg cggttggcga gcttcagcga
      181 gcgggcgagg gcggcctcgc gttcctcgcg ggcgcgcagg cgctcgctgg cgctgcggtg
      241 cgcgaccagc gcatccagcg tctcgcggaa ggccgcctgc accgtgcgcc ggtacgcgat
      301 ctcggcctgc gccgtgcgcg cctcggcgcc ctcgacgttc gcggcgatgc gcccgccggt
      361 gaacagcggc tgcacgaggc cggcggcgag cgaccaggtg ttggccgggc cggtgaacag
      421 atccgacagc gcctgactct cggcgccgta ggagccggtc agcgtcagcc gcgggaagta
      481 gtcggccttt gccgcgccga tgcgggcgtt ggcggcgacg agctgctgct cggcggagcg
      541 cacgtcgggc cggcgttcga gcaggtcgga gggcagagcc gccggcaccg agggcagcgg
      601 cggcagcttc tcctcgccgc cgagatcgcc gaccgggcgc tcgaccagcg cctgcggcgt
      661 gcgcccgagc agcacggcga gcgcgttgcg ggcggcggcg agctgctgtt cgagatcggg
      721 caggcgcgcc gcggcatcgg cggcttcggc ctcggcctgg ctggcttcga gttcggaaac
      781 cgaaccggca cggaaccgct tctgctgcag cccggcggtc tggcggcgcg aggtgacggt
      841 ctggcgggtg atgtcgagct gcgcggccag cgcgcgcgcc tcgaacaggc tgcgcgcgac
      901 ggtcgcggtc agcgacaggc gcacgccgtc gcgcgcggcc tcgctggcgg cgagttcggc
      961 gcgcgccgcc tcggtgccac gcgcgagccg cccccacagg tcgatctccc aggacaggtc
     1021 gagcgacacg cgccggtcgc tgctggtcag cggcgtgccg gccggcagcg gcgtcgagcc
     1081 gacgccgctg atgcgcgagc ggctctggtt cacgccgacg gtcgcatccg gcagctgctg
     1141 gccgcgggca ccgcgcaggc tggcgcgggc ctcgtcgatg cgcgcgaccg cttcggcgag
     1201 gtcgaggttg tgcgcgagcg cctcggcctc gaagccgtcg agcaccggat cgttgaagct
     1261 gcgccaccag tcatcggcga tcctcggcac gccggccgtg ccggccggca gctccagcgc
     1321 cgggcgctgg tagtcggggc cgaccatgca gccggccagc gccagtgcga cggcgagcgc
     1381 ggcgacccgt gcggcgacgc ggcgcgaccg gcggaacggg cgtgacaggg aacggatgcg
     1441 ctcagccatg atgcggctcc ggagccttcg cggacgggtc ggaggggtcg tgcgggtcag
     1501 ccgcagcgcc gtggtgatgc ggtttcggcg catggcgcac gacgaggcgg aagaacagcg
     1561 gcacgaagaa ggtcgcgagg aaggtcgcgg ccagcatgcc gccgatgacg ccggtgccga
     1621 tcgaatggcg gctcgccgcc ccggcgccgg tcgacagcgc gagcggcacg acgccgagca
     1681 cgaaggcgag cgaggtcatg atgatcggcc ggaagcgcag ccgtgcggct tcgagcgcgg
     1741 cctgcgcgac cggcacgccg tcctgcgcct tcagcagcgc gaactcgacg atcaggatcg
     1801 cgttcttcgc ggccaggccg atcagcgtca ccaggccgat ctggaagtac acgtcattgg
     1861 ccatgccgcg cacccagacc gcggccagcg cgccgaacag cgcgaacggc acggcgagca
     1921 cgacggcgac cggcagcgtc cagcgctcgt acagcgcggc gaggatcaaa aacaccatga
     1981 ccagaccgaa accgaacacc agcgtcgagg tgccgccggt caggcgctcc tgataggccg
     2041 agccggtcca caccagcgag tagtcctcgc cgagggtctg cgcggcgacc tgctccatcg
     2101 ccgccagcgc ctgcccggag ctgtagccgg gtgccggacc gccgacgatc ttcgcggccg
     2161 ggaacacgtt gtagcgatcg acgacctcgg ccccggcgct gcgcacgacc tcgaccagcg
     2221 ccgtcagcgg gatcatgcgg ccgtcggtcg agcgcacgta gacgtacttc aggtcatcgg
     2281 gcttctcgcg gtactcggcc tcggactgca cctgcacctt gaaggtgcgg ccgagcttgt
     2341 tgaagtcgtt gacgtacagc gcgccgaagg tcgcctgcat ggtctcgaac acggtgttga
     2401 ccggcacgcc gagcgcgcgc gccttctcgc gatcgagccg gatgtcgatc tgcggcacgc
     2461 tggcattgaa gatcgtgttc agcccggcga gctccgggcg ctggcccgcg gcggcgagga
     2521 acttctgcac ctcgccggcc agcgccttcg aaccgccgcc gccgcggctc tgcagatagg
     2581 cctcgaagcc gccggtcgtc gacatgccgc tgatcggcgg cgggttgaac gcgagcacga
     2641 tggcatcgcg gatctgcgcg ccctgcatga agaactgccc gaccagcgac tgcgcgtcct
     2701 gaccgggcgc ggtgcggtct tcccagtgct tcagcgggat gaaggcggcg ccgtagaacg
     2761 gcacgttcga gaacgacagc agatccagcc ccgacagcgc gatgacgttg tcgaccgcgg
     2821 ggtttgcgag cgcgaactcg ttgatgcggt cgttgacggc gatcgtgcgt tcgagcgagg
     2881 cgccgggcgg caggatcgcc atcgtcatca cgtagccctg atcctcatcc ggcaccagcg
     2941 agcccggcac gcgctggaac agcccccagg tcagcaccag catgccggcg aacagcgcca
     3001 cgccgagcag cgcgcgcttc aggaagaagc gcacgccggc cgaataacca ttggtgacgt
     3061 gctcgaagcc gcggttgaag gcgcgaaaga accgccccgg ctcgtgggcg acgggcttca
     3121 gcagcgtcgc gcacagcgcc ggcgtcagcg tcagcgcgac gaagcccgac agcacgaccg
     3181 acacggcgat cgtgatcgcg aactgcttgt acatctgccc ggccagtccg ccggcgaagc
     3241 cgaccggcac gaacaccgca cagagcacga gcacgatggc gatgaccggg ccgctgacct
     3301 cctgcatcgc ctggatcgtc gcctcgcgcg gcggcaggcg ctgggtgcgc atgatgcgct
     3361 cgacgttctc cagcacgacg atggcgtcgt cgacgacgat gccgatcgcc agcaccatgc
     3421 cgaacagcgt cagcgtgttg atcgagaagc cgagcaggta catgccggcg aaggtgccga
     3481 tcagcgacac cggcacggcc aggaacggga tgatggtcgc gcgcgcgctc tgcaggaaca
     3541 ggaacacgac gaggaacacc agacccatcg cctcggcgag ggtcttgacc acctcgcgga
     3601 tggagatctc gacgaagcgc gtcgtgtcat acggcgccgt ccactgcagg ccgaccggga
     3661 agtgccgggc cagctccttc atgcgcgcat cgacggcgcc ggcgaccgcg agcgcattgg
     3721 caccgggcgc gaggaagatg ccgatcgcga ccgtctcgtt gccgctgcgc ttggcgacca
     3781 tgccgtagtc ggcggcgcca agctcgatgc gcgcgacatc acggagccgc agcagcgtgc
     3841 cgtcggcctc ggcgcgcagc acgatcgcgc cgaattcctc cggcgtcgcc agacgcccct
     3901 gcgtcgtcac cgtgtaggtg aattcctgcg gcgtgcccat cggcgcctgc ccgaacttgc
     3961 cggccgcgaa ctgcgagttc tgctcgcgca ccgcggcggc gatgtcggac ggcgtcagcc
     4021 cgaggctcgc gagccggtcg ggccggatcc agatgcgcat cgagtagtcg cgcgccgaga
     4081 agatcatcgc gttgccgacg ccgttgatgc ggcgcagctc gtcgacgacg ttgaccagcg
     4141 cgtagttgct gatgaacacc gtgtcgaagc gcttgcttgg cgaatcgagc atgacgatct
     4201 gcagcaggtt cgagctctgt ttttcggcct tgacgccctg gcggcgcact tcctcgggca
     4261 gcgactgcag cgccgactgc acgcggttgt tgacgttgat cgtcgcctga tccggatcgg
     4321 tgccgatcgc gaagctggcg gcgatggtca gcgcgccggc cgaggagttc gagcgcacgt
     4381 acagcatgtc gtcgacgccg ttgatcgcct gttcgagcgg cgcggcgacg gtcgcggcga
     4441 ccacctcggc gctggcaccc gggtagaagg ccgtgacctg cacctcgggc ggcacgatgt
     4501 ccgggtattg cgcgatcggc agcgcgcgca tcgacgcgag gccggcgagc acgatgacga
     4561 tcgacagcac gctcgcgagg atcgggcgtt cgatgaagta gcgggaattc atgcgcgggc
     4621 tccccggggg tgggcgcggg acggcacggc gcgcgggcgg gcgcgcgccg gcgatgcaat
     4681 gggtgccaga gcgggttcag ccctgcggcg cgtggccgcc ggccgcggcg gccggtgccg
     4741 gtttcggctc gacgacgagc gcgccgggcg cgagcttcag caggttgtcg gtcgcgaccc
     4801 gctcgccgcc cttcagccct tcctcgacga tccagccctt gccgatcgag gcgccggtga
     4861 cgaccggccg gaactcgacc ttgtcgccgt cgccgatgac ccagacgaac tggccgtccg
     4921 gtccctgcat caccgcacgc tgcggcacga cgatcgcgcc ggggcgcgtg acgcccttca
     4981 gccgcacgcg cacgaactgg ccgggcagca gcgtggcatc ggggttggca accgtcgcgc
     5041 gcacgccgac cgtaccggtc tgcggatcga ccagcgtgtc ggtgaagtcg atccgcccgg
     5101 cctgcggatg cacgctgcca tcggcgagca cgacctcgac ctgcggtccg ccggccggca
     5161 tgcgcagccg gccctcggcg atcgccttgc ggatcatcag cgcctcgttc tcggcgtagg
     5221 tgaagcgcac gtaggccgga tcgacctggt tcagcgtcgt cagcgcgccg cttgcatcgc
     5281 cgggacgcac gaggctgcct tcggagcgca cctcgcggct ggtgatgccg gcgatcggag
     5341 cggtcacgcg cgtgtagccg aggtcgatct cggccgtgcg caccgcggcg cgcgcggact
     5401 tgaccgccgc actcgcctga tcgcgctcgg cctgcgcggc gtcgcggtcc ttctggctga
     5461 cggcccggtc ggcgaacagc ggctgcacgc gcttcagctc cttgtcagcc tgcgcaagcc
     5521 gcacctcctg ctcggcgaga tcggcacggg cgcgatcgag cgcggcctgg aaaggcgccg
     5581 ggtcgatcag gaacagcgtc tcgccggcct tgaccggctt gccttcggtg taggtgcgct
     5641 tctgcaggat gccctcgacg cgtgcgcgca cctgcacctc gcgcgaaccg ccgacctgcg
     5701 cgacgtattc gagcggcagc tcgacatcgg ccgcacggac gacactgcgc gcgacctcgg
     5761 gcggcggcat cgcgtggtta gcgctgtcat tgttggcgcg gctgtcgcag ccggcgacca
     5821 gcacgatcag ggcaccggcg aagatccata cggtgacgag gcgcatcagg atcaggtccg
     5881 gccggcggaa gagccgtgac agcgggcgcg tgaacaggcg gatcatgggc aggactcccg
     5941 gagcgcaccg gcggatgcgt gtgcgcggac aagcgccacg gcgcgacgtt cgatgatgtt
     6001 gcagtgcact ttacatacat tcatgtatgt atgcaaagcc ccgcgaagcg atacactgtc
     6061 cgcaaccggc accgactccg ccgttttccc gggattcacc ccgcaccgac atggtcagac
     6121 gcaccaaggc cgaggccgag cagacgcgct cgctgatcct cgacaccgcc gaacgcctgt
     6181 tcttcacgaa gggcgtctcg cgcaccacgc tcgccgagat cgcgacggcc gccgggctga
     6241 cgcgcggcgc gatctactgg catttcgcga acaagctcga actgttcaac gcgatgcacg
     6301 aacgcgtcgc gctgccgttc accgaggcga tggaaaagct gctgcacgcc gaagacccgc
     6361 tggcggcgct gcgcgagcgc tgcgtgctcg acctgcagga catcgccgcc tgcccgcaat
     6421 tgcagcaggt gctggcggtg ctgccgcaca agtgcgagta cgtcgacgaa ctcggcgagc
     6481 tgtgcctgcg cttcgaccag cagaacaagt gcttcgtcga gcacagtgaa accgtgctcg
     6541 cacgcgcgca cgcgctcggg cagttgcgcg atggtgtcga accgcggcgc gcggcgatcg
     6601 cgctgcacaa ctacatgctc ggcacgatca gcgactggct gttcgcgcgg gacctgcgct
     6661 accggctcgc cgacgaggcc ggcccgctgg tcgatctgtt cttcgccggc ctcgaacgtc
     6721 cggcctgacg cctgcagccg cacgcccttt tttgccggag accggaaaaa aacgaaggcc
     6781 gcaccgggca cggaccggtg cggccttcgt cttctggcag aggccgggtt ttcagcgcgt
     6841 cgccggcgcc ggctcgctcg ccgtagccac ggccggcgtc gtcgccaccg gcacggcggc
     6901 cggcgtcacc ggcgcgaccg gcggcaccga cggatcggcg gacggcgccg ccggcgtggc
     6961 ttccggggcc ggcgccggct cgatgaccgg gatcggcttc ggtttcggca gcgggccgtt
     7021 cagcttgccg tagatgtcgc tgtaatagac gacgtgaccg tcgatggcct cggccgggcg
     7081 gtagaccaga tagatcggaa tctgcggcga aggctcgacg acggtgcggc ggcggttgat
     7141 gcgcttctgg atctcatcct cgctcaggcc catcagccag gcggcgagcg gccggtactg
     7201 gtgcacgcgc acgcagcccg aggacagcgc ccgcaccggc ttgccgaagt cgttgcggtg
     7261 gttggtgtcg tgcaggtaga cgctcggcgt gtcggtgatc tcgaacttca gcttgccgag
     7321 cgcgttggaa tccccggccg gctggtacat ccggtagccg gcggcgtgga agccggcggc
     7381 gctgatcgga ccgatcgcat cgggcggaat ctggcggccg ccgcgatcga gcacgaccag
     7441 ccccttgcgc gtgaccgccg acggatcacg gccgaacagc gggatcaggt cttccttgat
     7501 gatggtcggc ggcggcgtcc aggtcgggtt gtacttgacc gccttcatga acaccgacag
     7561 ctccggcgtc gggcgcttgg gccgaccgac gacgacgttg gaactgagcg caagctgacc
     7621 ggactcgaag gcgcgcagct tgtaggacgg gatgttgacg atcaccagcc gcgggaccgt
     7681 gcgatcgatc ggctgcgcgg tttccgccgc cagcgtgtag cgcaggcgca ccagacgatc
     7741 ctcgcgcgac atcgacagct tcgcgaccgt ggacttgtcg aggctgccgg tttccttcag
     7801 gccgttgatg cgctggaaat cgacgagggc cgcggccagc gcgttgtcat caccgcgcgg
     7861 cacgtcgtaa ccccattcgg ccagccgcgc ccgcagcggc tcggcggcct tcggaccggc
     7921 gcccatgtgc cagctgcccg gggtcggcgg ttccggcgtg gtcagcgcct gcaggcgatc
     7981 gtgttcgcgc tggatcggat cgatgacgac ttcatcggcg tgaaccagcg cagcaaacgg
     8041 cagggaagcc acgaaggcga acagcgaggg caggaggaag ggacgcatgc tgcgctccgg
     8101 aggatcggcg gaatggaaaa ttctaccgtg agacggtccg gatgcgctcg gtcattgaca
     8161 tggttttcat ttgacataag gcatcttatg cggcc
//
LOCUS       DAOQIE010000173         8147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4516_length_8147_cov_7.546342, whole genome shotgun sequence.
ACCESSION   DAOQIE010000173 DAOQIE010000000
VERSION     DAOQIE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4516_length_8147_cov_7.546342"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1040)
                     /locus_tag="PK144_11355"
     CDS             complement(60..1040)
                     /locus_tag="PK144_11355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNF66371.1"
                     /translation="MSAHGPAVLEAVDLCRHYPVRRGLLQPVQTVRALDGVSFTLAAG
                     RTLAVVGESGCGKSTLARLLTLIEPPTSGALALLGDDCSASTAAQRREAQRRVQMVFQ
                     NPYGSLNPRQKIGAILAEPLKIHTKLDAAAREAAVRALMTRVGLRPEHHDRYPHMFSG
                     GQRQRVAIARALMLRPAVVVADEPVSALDVSIQAQVLNLLLELQEETGVAYVFISHDL
                     AVVRHIADEVLVMYLGRAVEQGPADAVFSAPRHPYTRALLAAAPRLDAGDSERVPLRG
                     ELPSPLSPPAGCAFHTRCPFADGECAQSRPAPRIVDGRLVACHHAERIGG"
     gene            complement(1037..2032)
                     /locus_tag="PK144_11360"
     CDS             complement(1037..2032)
                     /locus_tag="PK144_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF66372.1"
                     /translation="MALLEIENLCVDFGTEARPFRAVDRVSFTLERGEVLGIVGESGS
                     GKSVTSLALMGLIDFPGRVRAGRLYFDGEDLLALSERARRRLTGKDIAMVFQDPMSGL
                     DPCYTVGFQIMETLAVHEGGSRRARRQRARELLERVGIPDPASRLDAYPHQLSGGMSQ
                     RVMIALAIACNPSLLIADEPTTALDVTIHAQILDLLGELQVRNRMGMMLITHDLAVVA
                     GACSRLLVMYAGQVVESGTVGAVFAAPRHPYTAALLAALPERAEPGARRLYALPGVVP
                     GQYDRRQGCLLAPRCPHASAQCRAVEPALRFDADGRAVRCHTPLDAAGRPAGAAA"
     gene            complement(2048..2929)
                     /locus_tag="PK144_11365"
     CDS             complement(2048..2929)
                     /locus_tag="PK144_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006534626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF66373.1"
                     /translation="MEAVLPAPPAPWRENWRHFRANPGAFAGLIVIVVLIALALAAPW
                     IAPHAPDAQYRDALLKPPAWSAGGDPRFWLGTDDVGRDLLSRLLFGARLSLAVGCIVV
                     TLSLGAGIVLGLVAGFARGVVDALIMRLMDVMLALPSLLLAIAIVAVLGPSLVNAMVA
                     IAIVVLPHYVRLTRAAVLAELSKDYVTASELAGAGRLRQMFVNVLPNCLAPLIVQATL
                     GFSSAILDAAALGFLGLGAQPPTPEWGTMLADARQFIQRAWWVVSFPGLAILITVLAF
                     NLMGDGLRDALDPRLKR"
     gene            complement(3012..4022)
                     /locus_tag="PK144_11370"
     CDS             complement(3012..4022)
                     /locus_tag="PK144_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF66374.1"
                     /translation="MIGFLLRRGLLLLPTFLGVTAIAFALIHLIPGDPLEMMAGERGM
                     DPARHARMMAELGFDRPLWQQYLGYLGDLLHGDLGRSINTRQPVLGEFLTLFPATVEL
                     SLAAMLIATLVGLPVGILAAVRRGTLFDHGVMGVSLAGYSMPIFWWALLLILLFSVQL
                     GWTPVSGRISVMFYVEPVTGFLLIDTLLAGDVDAFLSALHHLVLPAIVLGTIPLAVIA
                     RMTRSAMLEVIREDYIRAARARGLSPLRVIGVHALRNALIPVVTVIGLQTGTLLAGAI
                     LTETIFAWPGVGKWLIDSIARRDYPAVQGGILLVAAVVMAVNLAVDLLYGLINPRIRH
                     GR"
     gene            complement(4057..5652)
                     /locus_tag="PK144_11375"
     CDS             complement(4057..5652)
                     /locus_tag="PK144_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF66375.1"
                     /translation="MSRRQTAVVLALAALLAPPLAQAAKTLVYCSEGSPEGFNAQFYT
                     AGTTFDAASQPIYNRLTEFERGATTLVAGLAESWTISPDGLVYTFNLRKGVKFHTTAK
                     FRPTRDFNADDVLFSFQRQLDPNHPFHKVSGGSYEYFEGMDMPAVIDRIEKLDDHTVR
                     FTLKRPEAPFLANIAMDFASIMSAEYADAMLKAGTPEVIDLEPVGTGPFQRVAYQKDA
                     VIRYKANPDYWRGKAPIDNLVFAITPDASVRYAKLDANECQVMSYPNPADLDRMRQDP
                     KVELLSQPGLNIGYLAFNTKKKPFDDVRVRQALNFAVNKAAIIEAVYQGAGTVAKNPI
                     PPTIWSYNDAVQDYAYDPQRAKQLLQQAGLADGFETDLWAMPVQRPYNPNARRMAEMI
                     QADWAKIGVKAKIVSYEWGEYLKRLKAGDHQTALMGWTGDNGDPDNFLATLLSCEAAD
                     NGSNYAKWCFQPYQDLVTKARQVSDPAERTQLYGQAQVVFKEQAPWITIAHSVVYQPV
                     RKEVQGFRIHPFGLNLFYGVDLK"
     gene            complement(5722..6888)
                     /locus_tag="PK144_11380"
     CDS             complement(5722..6888)
                     /locus_tag="PK144_11380"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66376.1"
                     /translation="MPARLALWYFAYFAAVGVFLPFWPVYLRELGFPAAAIGTLLALY
                     SASRLVAPHLWGWLADRSGRRMAIVRAGGAATVLCFNGVFAVGDSFAGQAAVMAVFCF
                     FWTAALPQFEAVTLAHLGRDAHRYGRIRLWGSIGFIVVAAGGGAVVAACGVLVIPWAM
                     LAGLVAILVAGLLVPEPPVARAQDSATPLGQLLRTKAVWALIATGFLQQAAHGPYYTF
                     FTLYLGEHGIGERTAGLLWALGVVAEVGLFAWLPHGLPRVGLRRLLQLAVALSVLRWL
                     LLGLAVEVWALLLVAQLLHAASFAAFHACTLQLVQQRFGPAHAGQGQALYSSLGTGAG
                     AAFGAWLAGTLWLPWGGALTFVVAAGCSALAWLLVQYGLHTLQTVDTPATNPGP"
     gene            complement(6982..7350)
                     /gene="cowN"
                     /locus_tag="PK144_11385"
     CDS             complement(6982..7350)
                     /gene="cowN"
                     /locus_tag="PK144_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-fixation sustaining protein CowN"
                     /protein_id="HNF66377.1"
                     /translation="MTAPASTILSATTLSATTLATTLAATTGAATTLAADRYVSFRGI
                     DFDGHCRALIETIERYHAGEGAREPFWGWFLDLRRNDPAKRDDLLLLSSLVNPLRERF
                     EAHGDAEALALLERAEEECF"
     gene            complement(7447..>8147)
                     /locus_tag="PK144_11390"
     CDS             complement(7447..>8147)
                     /locus_tag="PK144_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF66378.1"
                     /translation="RLRYAAPLPCLPPAAALTATPEFLHFLRTPAGRELAGQFTSQRL
                     ARHTLLSTPGDARDRVFIVLGGRLRVYLADDERELTLAFLEAGDVYATHTPTFVRTVS
                     ACTLLMLDTPRFAELLQRQPLLTPAIMRVLGRLLGNSVSIIENLAFRDVRTRLAHWLA
                     GQARRHGQHIDGMLAVPMPLTFADIAQLLGTTRQSVSAGIGELERAGLIVRRGRRLLL
                     VPDAQALLRGPGGS"
BASE COUNT         1347 a         2857 c         2874 g         1069 t
ORIGIN      
        1 acccctctcc cacgaggggc gaggggctga aaagcgaggc aagcgttccg gtgtgaccgt
       61 tatccgccga tgcgctcggc gtgatggcag gccaccagcc ggccgtcgac gatgcgcggt
      121 gccgggcgcg actgcgcgca ctcgccgtcg gcgaacgggc agcgggtgtg gaaggcgcag
      181 cccgccggcg gtgacagcgg cgacggcagc tcgccgcgca gcggcacgcg ctcgctgtcg
      241 cccgcatcca gccgcggcgc ggccgcaagc agcgcgcgcg tgtacggatg acgcggcgcg
      301 ctgaacaccg catcggccgg gccctgttcg accgcgcgac cgaggtacat gaccagcacc
      361 tcatcggcga tgtggcgcac gaccgccaga tcgtgcgaga tgaacacgta ggcgacgccg
      421 gtctcctcct gcagctccag cagcaggttc agcacctgcg cctggatcga cacgtcgagc
      481 gccgacaccg gctcgtcggc gacgacgacg gcgggcctga gcatcagcgc ccgggcgatg
      541 gcgacgcgct ggcgctggcc gccgctgaac atgtgcggat agcggtcgtg atgctcgggg
      601 cgcaggccga cgcgcgtcat cagcgcgcgc accgccgcct cgcgcgcggc ggcatcgagc
      661 ttcgtgtgga ttttcagcgg ctcggcgagg atcgcgccga tcttctggcg cgggttcagc
      721 gagccgtagg ggttctggaa caccatctgc accctgcgct gcgcctcgcg ccgctgcgcg
      781 gcggtgctcg cgctgcagtc gtcgccgagc agcgccagcg cgcccgaagt cggcggctcg
      841 atcagcgtca gcagccgcgc cagcgtcgac ttgccgcagc cggactcgcc gaccacggcg
      901 agcgtgcggc cggcggcgag cgtgaacgac acgccatcga gcgcacgcac ggtctgcacc
      961 ggctgcagca ggccgcgccg gaccgggtag tggcggcaca gatcgacggc ttcgagcacg
     1021 gccggaccgt gcgcgctcat gcggcggccc ccgccggccg gccggcggca tcgagcggcg
     1081 tgtggcagcg cacggcgcga ccgtcggcat cgaagcgcag cgccggctcg acggcccggc
     1141 actgtgcgct cgcgtgcggg cagcgcggtg ccagcaggca gccctggcgg cggtcgtact
     1201 ggccgggcac gacgccgggc agcgcgtaca ggcggcgcgc gccgggttcg gcgcgctcgg
     1261 gcagcgccgc gagcagcgcg gcggtatacg ggtggcgcgg agccgcgaac accgcaccga
     1321 ccgtgccgct ctcgaccacc tggccggcgt acatcaccag cagccggctg caggcgccgg
     1381 cgacgacggc cagatcgtgc gtaatcagca tcatgcccat gcggttgcgc acctgcagct
     1441 cgccgagcag atcgaggatc tgcgcatgga tggtcacgtc gagcgccgtg gtcggctcgt
     1501 cggcgatcag caggctcggg ttgcaggcga tcgcgagcgc gatcatcacg cgctggctca
     1561 tgccgccgga gagctggtgc ggatacgcat cgaggcggct cgccgggtcc gggatgccga
     1621 cgcgctccag cagctcgcgc gcgcgctgcc ggcgggcgcg gcggctgccg ccctcgtgca
     1681 cggccagcgt ctccatgatc tggaagccga ccgtgtagca ggggtcgagg ccgctcatcg
     1741 ggtcctggaa caccatcgcg atgtccttgc cggtcaggcg ccggcgcgcg cgctcggaca
     1801 gcgccagcag atcctcgcca tcgaaataca gccgcccggc gcgcacgcgg ccggggaagt
     1861 cgatcaggcc catcagcgcc agcgaggtca ccgacttgcc ggagccggac tcgccgacga
     1921 tgccgagcac ctcgccgcgt tcgagcgtga acgacacgcg atcgacggcc cggaacggcc
     1981 gcgcctcggt gccgaagtcc acgcacaggt tttcgatctc gagcagcgcc accggcgacg
     2041 gactccccta gcgcttcagg cgcggatcga gcgcatcgcg cagtccgtcg cccatcaggt
     2101 tgaaggccag caccgtgatc aggatcgcca gccccggaaa gctgaccacc caccacgcgc
     2161 gctggatgaa ctggcgcgca tcggcaagca tcgtccccca ctccggtgtc ggcggctgcg
     2221 cgccgaggcc gaggaaaccg agggcggcgg catcgaggat cgccgacgag aagccgagcg
     2281 tcgcctgcac gatcagcggc gccagacagt tcggcagcac gttgacgaac atctgccgca
     2341 gccgtccggc cccggccagt tcgctggcgg tcacgtagtc cttcgacagc tcggcgagca
     2401 cggccgcgcg cgtcaggcgc acgtagtgcg gcagcacgac gatcgcgatc gcgaccatgg
     2461 cgttgacgag gctcgggccg agcacggcga cgatcgcgat cgcgagcagc aggctcggca
     2521 gcgccagcat cacgtccatc agccgcatga tcagcgcatc gacgacgccg cgcgcgaagc
     2581 ccgcgaccag gccgagcacg atgccggcac cgagcgacag cgtcacgacg atgcagccga
     2641 ccgcgagcga cagccgcgcc ccgaacagca ggcgcgacag caggtcgcgg ccgacatcgt
     2701 cggtgccgag ccagaagcgc ggatcgccgc cggcgctcca ggccggcggt ttcagcagcg
     2761 catcgcggta ctgcgcatcg ggcgcgtgcg gcgcgatcca cggcgcggcg agcgccagcg
     2821 cgatcagcac gacgatgacg atcagcccgg cgaaggcacc gggattggcg cggaagtgcc
     2881 gccagttctc gcgccacggc gccggcggcg ccggcagcac ggcctcgatt tcctgcagcg
     2941 cctcgtggct gccggccgcg aagcttccgg ccggcacggt ggccgccggg acctgtggct
     3001 cccccggacg ctcagcggcc atggcggatg cgcgggttga tgaggccgta gagcaggtcg
     3061 acggcgaggt tcacggccat caccaccgcc gcgaccagca ggatgccgcc ctgcaccgcc
     3121 gggtagtcgc gccgcgcgat cgagtcgatc agccacttgc cgacgcccgg ccacgcgaag
     3181 atggtctcgg tcaggatcgc gccggcgagc agcgtgccgg tctgcaggcc gatgacggtc
     3241 acgaccggga tcagcgcgtt gcgcagcgca tgcacgccga tcacgcgcag cggcgacagg
     3301 ccgcgggcgc gggcggcacg gatgtagtcc tcgcggatca cttcgagcat cgccgagcgc
     3361 gtcatgcgcg cgatcaccgc gagcggaatc gtgccgagca cgatcgccgg cagcacgaga
     3421 tggtgcagcg ccgacaggaa ggcatcgaca tcgccggcga gcagcgtgtc gatcagcagg
     3481 aagccggtga ccggctcgac gtagaacatc accgagatcc gccccgacac cggcgtccag
     3541 ccgagctgca ccgagaacag caggatcaag agcagcgccc accagaagat cggcatcgaa
     3601 tagccggcga gcgacacgcc catgacgccg tgatcgaaca gcgtgccgcg gcgcacggcc
     3661 gcgaggatgc cgaccggcag cccgaccagc gtcgcgatca gcatcgccgc gagcgacagc
     3721 tcgacggtcg ccggaaacag cgtcaggaac tcgccgagca ccggctggcg ggtgttgatc
     3781 gagcggccga ggtcaccgtg cagcaggtcg ccgagatagc cgaggtactg ctgccacagc
     3841 ggccggtcga aaccgagttc ggccatcatc cgcgcgtggc gcgccgggtc catgccacgc
     3901 tcgccggcca tcatctccag cggatcgccg gggatcaggt ggatcagcgc gaacgcgatc
     3961 gccgtgacgc cgaggaaggt cggcagcagc agcagcccgc ggcgcagcag gaagccgatc
     4021 acgccggccg ccccggccgc ggatgcgcag gccgcatcac ttcaggtcca cgccgtagaa
     4081 caggttcagc ccgaaggggt gaatcctgaa accctgcact tccttgcgca ccggctggta
     4141 gacgaccgag tgcgcgatcg tgatccacgg cgcctgctcc ttgaacacca cctgcgcctg
     4201 tccgtagagc tgcgtgcgct cggccggatc gctgacctgg cgggccttcg tgaccaggtc
     4261 ctgatacggc tggaagcacc acttcgcgta gttgctgccg ttgtcggcgg cctcgcagga
     4321 cagcagcgtc gcgaggaagt tgtccgggtc gccgttgtcg ccggtccagc ccatcagcgc
     4381 tgtctggtga tcgccggcct tcaggcgctt gaggtactca ccccattcgt agctgacgat
     4441 cttcgccttc acgccgatct tcgcccagtc ggcctggatc atctcggcca tgcgtcgcgc
     4501 gttcgggttg tacgggcgct gcaccggcat cgcccacagg tcggtctcga agccgtcggc
     4561 gaggccggcc tgctgcagca gctgcttcgc gcgctgcggg tcgtaggcgt agtcctgcac
     4621 cgcgtcgttg tacgaccaga tggtcggcgg gatcgggttc ttcgcgaccg tgccggcgcc
     4681 ctgatagacg gcctcgatga tcgcagcctt gttgacggcg aaattcagcg cctgccgcac
     4741 gcgcacgtca tcgaagggtt tcttcttggt gttgaaggcc agatagccga tgttcaggcc
     4801 cggctggctc agcagctcga ccttcgggtc ctgacgcatg cgatccagat cggccggatt
     4861 cggatagctc atgacctggc attcgttggc gtcgagcttg gcgtagcgca ccgaggcatc
     4921 gggcgtgatc gcgaacacca gattgtcgat cggcgccttg ccgcgccagt aatcgggatt
     4981 ggccttgtag cggatcactg catccttctg gtacgcgacg cgctggaacg gcccggtgcc
     5041 gaccggttcg aggtcgatga cttcgggcgt gccggccttc agcatcgcat cggcgtattc
     5101 ggccgacatg attgacgcga aatccatcgc gatgttggcg agaaacggcg cctccgggcg
     5161 cttcagcgtg aagcgcaccg tgtgatcgtc gagcttctcg attctgtcga tcaccgccgg
     5221 catgtccatg ccttcgaagt attcgtagct gccgcccgag accttgtgga acgggtggtt
     5281 cggatcaagc tggcgctgga acgagaacag cacgtcatcg gcgttgaagt cgcgcgtcgg
     5341 cctgaacttc gccgtcgtgt ggaatttgac gcccttgcgc aggttgaacg tgtagacgag
     5401 gccatccggc gagatcgtcc atgactcggc gagcccggcg accagcgtcg tcgcaccgcg
     5461 ctcgaactcg gtcagccggt tgtagatcgg ctgcgaggcg gcatcgaagg tcgtgccggc
     5521 cgtatagaac tgcgcgttga agccctcggg actgccttcc gagcagtaga ccagcgtttt
     5581 cgcggcctgg gcgagcggcg gcgcgagcag ggcggcaagg gcaagaacga cggcggtctg
     5641 acgacgggac atcatggtct ggcgactccg cagcacggtg aatggcgcgc agcgtagccc
     5701 agtggcacgc agcaaagcgg ttcaggggcc cggattcgtc gccggggtgt caactgtctg
     5761 caacgtgtgc aggccgtact gcaccagcag ccaggccagc gccgaacagc cggccgccac
     5821 gacgaaggtc agcgccccgc cccagggcag ccacagcgtg ccggccagcc acgcgccgaa
     5881 cgccgccccg gccccggtgc cgagactcga atacagcgcc tgtccctgcc cggcgtgcgc
     5941 cggcccgaag cgctgctgca cgagctgcag cgtgcaggcg tgaaaggccg cgaagctcgc
     6001 cgcatgcagc agctgtgcga cgagcagcag cgcccacacc tcgaccgcca gcccgagcag
     6061 cagccagcgc agcacgctca gcgccaccgc cagctgcagc agccgccgca ggccgacgcg
     6121 cggcagcccg tgcggcagcc acgcgaacag cccgacctcg gccaccacac cgagcgccca
     6181 cagcagcccg gccgtgcgct cgccgatgcc gtgctcgccg agatacagcg tgaagaacgt
     6241 gtagtacgga ccgtgcgcgg cctgctgcag gaagccggtc gcgatcagcg cccagaccgc
     6301 cttcgtgcgc agcagctgcc cgagcggtgt cgccgaatcc tgcgcacgcg cgaccggcgg
     6361 ctccggcacc agcagcccgg ccacgaggat cgcgaccagc ccggccagca tcgcccacgg
     6421 gatcacgagc accccgcatg cggccaccac cgccccgccg ccggccgcga cgacgatgaa
     6481 gccgatcgac ccccacagcc ggatgcgccc gtagcggtgc gcatcacgcc ccagatgcgc
     6541 gagcgtcacc gcctcgaact gcggcagcgc cgccgtccag aagaagcaga acaccgccat
     6601 caccgccgcc tgcccggcaa aactgtcgcc gaccgcgaac acgccgttaa agcacagcac
     6661 cgtcgccgcg ccgccggccc gcacgatcgc catgcgccgc ccgctgcgat ccgccagcca
     6721 cccccacaga tgcggcgcca ccagccgcga ggccgaatac agcgccagca gcgtgccgat
     6781 cgctgccgcc gggaagccca gctcgcgcag gtacaccggc cagaacggca ggaacacgcc
     6841 gacggcggcg aaataggcga agtaccagag ggcaaggcgg gcgggcatga cgacagtgcg
     6901 cagtcggcag aggggcgcgc atcgtagcgg aagcgcgcgc tctgccgtgg cttcctcatg
     6961 ccaccagccg atggcaccgg ctcagaagca ctcctcctcc gcgcgttcca gcaaggccag
     7021 cgcctcggca tcgccatgcg cttcgaagcg ttcgcgcagc ggattgacga ggctcgacag
     7081 cagcagcagg tcgtcgcgct tcgccggatc gttgcggcgc aggtcgagaa accagcccca
     7141 gaacggttcc cgtgcgcctt ccccggcgtg gtagcgctcg atggtttcga tcagcgcgcg
     7201 gcagtggccg tcgaagtcga tgccgcggaa cgagacgtag cgatcggcgg ccagggtcgt
     7261 ggcagccccg gtcgtggcgg ccagagtcgt tgcaagggtc gttgcagaca gggttgttgc
     7321 agacaggatc gtgctggcag gggcggtcat ggacgggttc cgggggatgg cgggaaaggg
     7381 aggagcagca tccctcgtgc ggctgcgatc cgtccgtcgg ccggccgacg tttccgggcc
     7441 tgaatttcag cttccgcccg gtccgcgcag caaggcttgc gcatccggca ccagcagcag
     7501 gcgccggccg cggcgcacga tcagcccggc gcgttccagt tcgccgatgc cggccgagac
     7561 gctctggcgc gtggtgccga gcagttgcgc gatgtcggcg aacgtcagcg gcatcggcac
     7621 ggccagcatg ccgtcgatgt gctggccatg acggcgggcc tgcccggcga gccagtgcgc
     7681 gagccgcgtg cgcacgtcgc gaaatgccag gttctcgatg atcgagaccg agttgccgag
     7741 caggcgtccg agcacgcgca tgatcgccgg cgtcagcagc ggctggcgct gcagcagttc
     7801 ggcgaagcgc ggcgtgtcga gcatgagcag cgtgcaggcg ctgaccgtgc gcacgaaggt
     7861 cggcgtatgc gtcgcgtata cgtcgccggc ttcgaggaag gcgagcgtca gctcgcgctc
     7921 gtcatcggcc agatacacgc gcagccgccc gccgagcacg atgaacacgc gatcccgggc
     7981 atcgcccggc gtgctgagca gcgtgtggcg cgcgaggcgc tgactggtga actgtccggc
     8041 cagctcgcgg ccggccggtg tgcgcaggaa gtgcaggaac tcgggagtgg cggtcaaggc
     8101 ggcagccggc ggcagacaag gaagcggcgc agcgtaccgg agccttg
//
LOCUS       DAOQIE010000174         8139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4528_length_8139_cov_6.988248, whole genome shotgun sequence.
ACCESSION   DAOQIE010000174 DAOQIE010000000
VERSION     DAOQIE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4528_length_8139_cov_6.988248"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1281)
                     /gene="hemL"
                     /locus_tag="PK144_11395"
     CDS             complement(1..1281)
                     /gene="hemL"
                     /locus_tag="PK144_11395"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNF66379.1"
                     /translation="MTRSETLFAEAQQYIPGGVNSPVRAFRGVGGTPIFFKRGEGAYL
                     IDEDDRRYIDYVGSWGPMIAGHAHPQILAAVIDAAQHGLSFGAPTAIESVLARKVIEL
                     VPSIELVRMCSSGTEATMSAIRIARGYTGRDRIVKFEGCYHGHSDSLLVKAGSGMLTL
                     GVPTSPGVPAELAQHTLTLPFNDLDALAACFAEYGSTIAGVIVEPVAGNMNCIPPVPG
                     FLEALRTHCDAAGAVLIFDEVMTGFRVARGGAQQHYGITPDMTTLGKVIGGGMPVGAF
                     GGKRAIMEKLAPLGPVYQAGTLSGNPVAMAAGLATLALIEAPGFHERLSALSARLCAG
                     LEQAAGAAGIALTTNHVGGMFGFFFTDKKVSSYAEATSCDVERFKRFFHGMLAEGVYL
                     APSAFEAGFVSSALTDADIDATIAAAERVFGRIG"
     gene            1664..2914
                     /locus_tag="PK144_11400"
     CDS             1664..2914
                     /locus_tag="PK144_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNF66380.1"
                     /translation="MTPHRLAPAAAGLLLCSSLPAQATIVLQTLTDAVAAPMYAAATA
                     ADPAHLYVAERDGTVRRVERTSGAVEATPFLDIRSQVNTGGEGGLLGIAFDPGYAGNG
                     HFYVNYLDNSQQTRVVRYTAAGGVVDPGSAQTVIAISQPQLNHNGGWIGFGPDNQLYV
                     ATGDGGGSDDNDAGHTPGIGNAQDLSSPLGKILRLDVDSDAFPADPQRNYAIPTDNPL
                     LGGAAAPSEIWAYGLRNPFRAGFDRLTGDLWIGDVGQLAREEIDFQPAGIGGQDYGWR
                     LREGTIATPSGGVGGPNPDAVDPVHEYDHTVGRSVIGGTVYRGSIAELYGRYFFADFN
                     AGRLWTLDGSGVTDWTDLLTAIGAPLLGIVGFSEDVDGELYLMDLGGAIYRIAEIPAP
                     GGLALLLAGLGLLRLRAARPPVLG"
     gene            3083..4198
                     /locus_tag="PK144_11405"
     CDS             3083..4198
                     /locus_tag="PK144_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNF66381.1"
                     /translation="MTPAPTLFRTLLLAALVGVSGSAVADRVKDLASVGGVRSNQLIG
                     YGLVVGLAGTGDQTSQTPFTVQSLRNMLTGLGVVLPANISLQLKNVAAVSVHAELPPF
                     AKAGQTVDVTVSSLGNAKSLRGGSLLLSPLRGTDGEVYAIAQGNVVVGGLGVEGAGSS
                     ITVNVPSSGRIANGATVERPVPDGMTHAEGPLMLNLRQSDFTTAQRVADAINRKLHSQ
                     VAAPMDATTITVDAPADPRYRVAFLASIENIEVEPAETAAKVIVNSRTGTVVVGSNVR
                     VFPAAITHGSLTVTITDDTTVSQPSPLSGGSTVVVPRQNIDVKEEKRPMFLFKPGVKL
                     EDIVKAVNQIGASPSDLVAILEALKAAGALRAELLVI"
     gene            4202..5263
                     /locus_tag="PK144_11410"
     CDS             4202..5263
                     /locus_tag="PK144_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF66382.1"
                     /translation="MATLTASAPVSLPSVSAPRLLRAALPLLIAAPVVLGVQAGGVWV
                     LAQLAKPEPAASAPVATTAAPRTAAPLFASTSSSVPVLAKAPAAEAAAPPAPVISRAA
                     TPEVPAGERPVPAASVEPGAGKPVTAAPAQAPAPGPDAGPVQAASAPEAPVARVEAVA
                     PKPAPASADTADAAPPAVSPVSPTPPAVSPVTRPAASVLYVPPPYVPVAPYAVAAAQA
                     PARAAVTAVATPAPVAHPAGDKVPAPAPAGTEPERQELLGPDWVRAQPVNNRTLQLMA
                     GSSLEVLREYVRSHDFADVPVAYIPSIRDGQPWYYLIAGSFASVQESSAAERVLKNVQ
                     SGLKPWTRRFGGMQALIKP"
     gene            5279..6310
                     /gene="flgJ"
                     /locus_tag="PK144_11415"
     CDS             5279..6310
                     /gene="flgJ"
                     /locus_tag="PK144_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNF66383.1"
                     /translation="MSTNALSSAAGTPPPVPTALGDFAGLARLRTQSAQQTPEAQREV
                     ARQFEALFIQQMLKTMRAASPGDSLVDSDASRTMRDFHDQKLAESLSQRGDFGIAQLL
                     LKQIAPDAASGRQAGPAIPAAGPAPKLDANALEVLRRVERVRDDGRLEATTAPVASAA
                     YKPGTPEAFVREVWPHAQAAARQLGVAPEVLVAQAALESAWGRSMPTRADGTPSFNLF
                     GIKADRRWQGARVENATTEFVGGRSVREVAGFRAYGSWGESFADYARFIQDNPRYRQA
                     LASAADNSAYLHELHKAGYATDPGYAAKIKRILGGDALNEALRGMGVNTGINTGVNLS
                     AGTAVPGPG"
     gene            6329..>8139
                     /gene="flgK"
                     /locus_tag="PK144_11420"
     CDS             6329..>8139
                     /gene="flgK"
                     /locus_tag="PK144_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNF66384.1"
                     /translation="MADLLNTGLSSLRAAQLALSTAGHNIANVNTEGYSRQRATLETR
                     LPQYVGEGFIGTGVNVATISRSFDKFLTQQVRSHTATQSQLDVQQGLTEQVDSLLSDG
                     QASLAPLLQGVYDAMQAVAEAPAATAPRQTLLAQAQSLTDRFHEIDSQLGTLRTNVNQ
                     GIRDAVDQINDYAGALAKVNKEIGIASGTGGSSAPPNDLFDERDRLLQKLSEQVGVQA
                     VIAGNGTANVYVGSGQALVTGFDVQKLEVGPDAYTGEDLAVRYAGQRQGTDLAPYLTG
                     GKIGGYVAFRDGMLSRVQGDVGLFAAGIADAVNSQQTRGLDLQGRLGEAMFTTSAARV
                     FAHDSNDGDAQVSVTIARVPDLQRSDYELRYANGNYSLLRLSDSQVIATGSGPGLTGD
                     GLDIDIPSGTPANGDRYLIQPTRLAASQIEVALIGTDQIAAAAPIATAAARANIGTGS
                     ISAGTVNTLPTTASDVLKPVEIRFTSETTYDLVDVSDPNNPVTLQSASAYSASAGATI
                     GVNGWEVRVSGQPQNGDVFTVGPNTAGSGDNRNMLALAGLATKRVLIGSTADLKGFYA
                     GVIGDIGTQSQRISTAVTAQTALLNQAKQARESASGV"
BASE COUNT         1279 a         2929 c         2822 g         1109 t
ORIGIN      
        1 tcagccgatc cgcccgaaca cccgttccgc cgccgcgatc gtcgcgtcga tgtcggcatc
       61 ggtcagtgcc gacgaaacaa accccgcctc gaacgccgag ggcgccagat acacgccttc
      121 ggcgagcatg ccgtggaaga agcgcttgaa gcgttccacg tcgcagctcg tggcttcggc
      181 gtagctcgac acctttttgt cggtgaagaa gaacccgaac atgccgccga cgtggttcgt
      241 cgtcagcgcg atgccggcgg caccggcggc ttgctccagc cccgcgcaga gccgtgccga
      301 cagcgcactc aggcgctcgt ggaagcccgg cgcctcgatc agtgcgagcg tcgcaaggcc
      361 cgcggccatc gccaccggat tgcccgacag cgtgccggcc tgatagaccg ggccgagcgg
      421 ggcgagcttt tccatgatcg cgcgcttgcc gccgaaggcc ccgaccggca tgccgccgcc
      481 gatgaccttg ccgagcgtgg tcatgtccgg cgtgatgccg tagtgctgct gcgcgccgcc
      541 cctggcgacg cgaaagccgg tcatgacctc atcgaagatc agcacggcac cggccgcgtc
      601 gcagtgcgtg cgcagggctt cgaggaagcc cggcaccggc gggatgcagt tcatgttgcc
      661 ggcgaccggc tcgacgatga cgccggcgat cgtgctgccg tactcggcaa agcaggcggc
      721 gagcgcgtcg aggtcgttga acggcagcgt cagcgtgtgc tgcgcgagtt ccgccggcac
      781 gcccggcgag gtcggcacgc cgagcgtcag catgccggag ccggccttca cgagcagcga
      841 atccgaatgg ccgtggtagc agccctcgaa cttcacgatc cggtcgcgtc cggtgtagcc
      901 gcgcgcgatg cggatcgcgc tcatcgtcgc ctcggtgccc gaggagcaca tgcgcaccag
      961 ctcgatcgac ggcaccagct cgatgacctt cctcgccagc accgactcga tcgcggtcgg
     1021 cgccccgaac gacagcccgt gctgcgcggc atcgatgacg gcggcgagga tctgcgggtg
     1081 cgcgtggccg gcgatcatcg gcccccagga gccgacgtag tcgatgtagc gccggtcgtc
     1141 ctcgtcgatc aggtacgcgc cttcgccacg cttgaagaag atcggcgtgc cgccgacgcc
     1201 gcggaaggca cgcacgggcg agttcacgcc gccgggaatg tactgctgcg cctcggcgaa
     1261 cagggtttcg gaacgggtca tcgcggaagt ctcgctggcc ggtgaagcgc cggcagccgc
     1321 cggcggggaa gggcgacctt gtagcgcagc cgccggcaaa cgcaaacccc ggcatggccg
     1381 gtgtgtgggt cgcgattaaa agcccgtaag ccgcttgcga cgccggcatg accccataag
     1441 gtttgccgca ggtccggcca cggccggacc cggccgcatg gacgtcgcag gcagcaccgg
     1501 ggtgcggcgc cctgccgaag caccgggaaa caccggttgc cgggtcgatg acttgccgac
     1561 gggagttgcg ccgcaccagc acgcggcgcc ggggaaacag aaagcgcgga tgccggcgat
     1621 gcccggcatc cgccggaatc cgcgcgaccc gaggacgctt ccgatgactc cgcaccgcct
     1681 tgcaccggcc gccgccggcc tgctgctttg ttccagcttg cccgcccagg ccacgatcgt
     1741 gctgcagacg ctgaccgatg ccgtcgcggc accgatgtac gccgccgcga ccgctgccga
     1801 tccggcacac ctctatgtcg ccgagcgcga cggcaccgtg cgccgcgtcg agcgcacgag
     1861 cggtgcggtc gaggcgacgc ctttcctcga catccgcagc caggtcaaca ccggcggcga
     1921 gggcgggctg ctcggcatcg ccttcgatcc gggctacgcc ggcaacgggc acttctacgt
     1981 caactacctc gacaactcgc agcagacgcg cgtcgtgcgc tacacggccg ccggtggcgt
     2041 cgtcgatccg ggctcggcgc agacagtgat cgcgatcagc cagccgcagc tcaaccacaa
     2101 cggcggctgg atcggcttcg ggccggacaa ccagctgtac gtcgcgaccg gcgatggcgg
     2161 cggttccgac gacaacgacg ccggccacac gccgggcatc ggcaacgctc aggacctgag
     2221 ctccccgctc ggcaagatcc tgcgcctcga cgtggacagc gacgccttcc cggccgaccc
     2281 gcagcgcaac tacgcgatcc cgaccgacaa cccgctgctc ggcggcgcgg ccgcgccgag
     2341 cgagatctgg gcctacggcc tgcgcaatcc gtttcgcgcc ggcttcgacc ggctgaccgg
     2401 cgatctgtgg atcggcgatg tcggccagct cgcgcgcgag gagatcgact tccagccggc
     2461 cggcatcggc ggtcaggact acggctggcg gctgcgcgag ggcacgatcg cgacgccgtc
     2521 gggcggcgtc ggcgggccga atcccgatgc cgtcgatccg gtgcacgagt acgaccacac
     2581 ggtcgggcgc tcggtgatcg gcggcaccgt ctaccgcggc tcgatcgccg aactctacgg
     2641 ccgctacttc ttcgccgact tcaacgccgg ccggctgtgg acgctcgacg gcagcggcgt
     2701 caccgactgg acggacctgc tgacggcgat cggggcgccg ctgctcggca tcgtcggctt
     2761 ctcggaggat gtcgacggcg agctgtacct gatggatctc gggggcgcga tctaccgcat
     2821 cgccgagatt ccggcaccgg gtggcctggc gctgctgctg gccgggctcg ggctgctgcg
     2881 gctgcgggcg gcacgacctc cggtgctggg ctgaagcctc cgggcgtccg gcgctatccg
     2941 ggcccgcgtc ggccgcacgg cgccggaaat ccggcggaag ttcatcgtca tccataaaaa
     3001 ataaataaaa tcaatgtatt gatctatggc ctgaatcctg cttgaagcgg cggcatccac
     3061 gccgtttcgc aggattcccg tcatgacccc agccccgacc ctgttccgta cgctgctgct
     3121 cgccgccctc gtcggcgtat ccggcagcgc ggtcgccgac cgcgtcaagg atctggcgag
     3181 tgtcggcggc gtgcgcagca accagctgat cggctacggc ctggtcgtcg gtctggccgg
     3241 taccggcgac cagacctcgc agacgccgtt cacggtgcag tcgctgcgca acatgctgac
     3301 cggcctcggc gtcgtgctgc cggccaacat cagcctgcag ctgaagaacg tcgccgcggt
     3361 gtcggtgcat gccgagctgc cgccgttcgc gaaggccggc cagaccgtcg acgtgaccgt
     3421 gtcctcgctc ggcaacgcca agagcctgcg cggcggttcg ctgctgctgt cgccgctgcg
     3481 cggcaccgac ggcgaggtct acgcgatcgc gcagggcaac gtcgtcgtcg gcgggctcgg
     3541 cgtcgagggc gccggttcca gcatcaccgt caacgtgccg agctccgggc gcatcgccaa
     3601 cggcgcgacc gtcgagcgcc cggtgccgga cggcatgacg cacgccgaag ggccgctgat
     3661 gctgaacctg cgccagagcg acttcacgac cgcgcagcgc gtcgccgacg ccatcaaccg
     3721 caagctgcac agccaggtcg ccgcgccgat ggatgcgacg acgatcacgg tcgatgcacc
     3781 ggccgatccg cgttaccgcg tcgccttcct tgccagcatc gagaacatcg aggtcgagcc
     3841 ggccgagacg gcggcgaagg tcatcgtcaa ctcgcgcacc ggcaccgtcg tcgtcggcag
     3901 caacgtgcgc gtgttcccgg ccgcgatcac gcacggcagc ctgaccgtga ccatcaccga
     3961 tgacacgacg gtcagccagc cgtcgccgct cagcggcggc agcaccgtcg tcgtgccgcg
     4021 ccagaacatc gacgtgaagg aagagaaacg gccgatgttc ctgttcaagc ccggcgtgaa
     4081 gctcgaagac atcgtcaagg ccgtcaacca gatcggtgcc tcgccgtcgg atctggtcgc
     4141 gatcctcgaa gccctgaagg ccgccggtgc gctgcgcgcc gaactgctgg tgatctgaac
     4201 catggccacg ctgactgctt ccgcccccgt ctccctgccg tccgtctccg ctccccggct
     4261 gctgcgcgcc gcgctgccgc tgctgatcgc cgcgccggtc gtgctcggcg tgcaggccgg
     4321 cggcgtctgg gtgctcgcgc agctcgcgaa acccgagccg gccgccagcg ccccggtcgc
     4381 cacgacggcg gcgccgcgca cggcagcgcc gctgttcgcc tccacgtctt catcggtgcc
     4441 ggtgctcgcg aaggcaccgg ccgccgaagc ggctgcgccg cccgctccgg ttatcagccg
     4501 tgcagcgacg ccggaagtgc cggccggcga acgcccggtg ccggcggcaa gcgtggagcc
     4561 gggcgccggc aaaccggtga ctgccgcacc ggcccaggct ccggctccgg gcccggatgc
     4621 agggccggtg caggcggcga gcgcgccgga agcgccggtc gcccgggtgg aagcggtggc
     4681 gccgaagccg gcacctgcct cggcggatac ggcggatgcc gcaccgccgg ccgtgtcgcc
     4741 ggtgtcgccg acgccgccgg ccgtcagtcc ggtgacgcgg ccggcggcga gcgtgctgta
     4801 cgtaccgccg ccgtatgtgc cggtggcacc gtatgcggtc gccgcagcgc aggccccggc
     4861 gcgcgccgcc gtgacggcgg tcgcgacccc ggcgccggtc gcgcatccgg ccggagacaa
     4921 ggtccctgcg ccggcgccgg ccgggacgga gcccgagcgc caggaactgc tcggccccga
     4981 ctgggtgcgg gcacagccgg tgaacaaccg cacgctgcaa ctgatggcgg gcagttcgct
     5041 agaggtgctg cgcgaatacg tgcgcagcca cgacttcgct gacgtgccgg tcgcctacat
     5101 cccgtcgatc cgcgacggcc agccctggta ctacctgatc gccggcagct ttgcgtcggt
     5161 gcaggaatcc tcggccgccg agcgggtgct gaagaacgtg cagtccggcc tgaagccgtg
     5221 gacgcggcgc ttcggcggca tgcaggccct gatcaagccg taagcggagg ccgcagccat
     5281 gtcgacgaat gcgctcagct ccgccgccgg cactccgccg ccggtgccga ccgcgctcgg
     5341 ggatttcgcc ggactggcgc ggctgcgcac gcagagcgcg cagcagacgc cggaagccca
     5401 gcgcgaggtt gcccgccagt tcgaggcgct gttcatccag cagatgctga agaccatgcg
     5461 ggccgcgagc cccggcgatt cgctggtcga cagcgacgcc agccgcacga tgcgcgactt
     5521 ccatgaccag aagctggccg agagcctgtc gcagcgcggc gacttcggca tcgcgcagct
     5581 gctgctgaag cagatcgcgc cggatgccgc gagcggccgt caggccgggc cggcgatccc
     5641 ggccgccgga ccggcgccga agctcgatgc caatgcgctg gaggtgctgc gccgcgtcga
     5701 gcgcgtgcgc gacgacggca ggctcgaagc gacgacggcg ccggtggcga gcgccgccta
     5761 caagcccggc acgccggagg ccttcgtgcg cgaggtctgg ccgcacgcgc aggccgcggc
     5821 gcgccagctc ggcgtcgcgc ccgaggtgct ggtcgcgcag gcggcgctgg aatcggcctg
     5881 gggccgctcg atgccgacgc gcgccgacgg cacgccgagc ttcaacctgt tcggcatcaa
     5941 ggccgatcgc cgctggcagg gcgcgcgcgt cgaaaacgcc acgaccgagt tcgtcggcgg
     6001 caggtcggtg cgcgaggtcg cgggcttccg cgcctatggc tcgtggggcg agagcttcgc
     6061 ggactacgcg cgcttcatcc aggacaaccc gcgctaccgg caggccctcg ccagtgccgc
     6121 cgacaacagc gcctacctgc acgaactgca caaggccgga tacgccaccg atccgggcta
     6181 cgccgccaag atcaaacgca tcctcggcgg tgacgcgctg aacgaggcgc tgcgcggcat
     6241 gggtgtgaac acgggcatca acacgggtgt gaacctgtcc gccggcaccg cggtgccggg
     6301 tccgggctga cgcgagagga gggccgccat ggccgatctg ctgaataccg gcctgtccag
     6361 cctgcgtgcc gcgcagctcg cgctgtcgac cgccggccac aacatcgcca acgtcaacac
     6421 cgagggctac agccgccagc gcgccacgct ggaaacccgg ctgccgcagt acgtcgggga
     6481 aggcttcatc ggcaccggcg tcaacgtcgc gacgatctcg cgcagcttcg acaagttcct
     6541 gacccagcag gtgcgctcgc acaccgcgac gcagagccag ctcgacgtgc agcagggcct
     6601 gaccgagcag gtcgacagcc tgctgtccga tggtcaggcg agcctcgcgc cgctgctgca
     6661 gggtgtgtac gacgcgatgc aggcggtcgc cgaggccccg gctgcgaccg cgccgcgcca
     6721 gaccctgctc gcgcaggcgc agtcgctgac cgaccgcttc cacgagatcg actcgcagct
     6781 cgggacgctg cgcaccaacg tcaatcaggg catccgcgat gcggtcgacc agatcaacga
     6841 ctatgccggc gcgctcgcga aggtcaacaa ggagatcggc atcgccagcg gcaccggcgg
     6901 cagttcggcg ccgccgaacg atctgttcga tgagcgcgac cggctgctgc agaagctctc
     6961 cgagcaggtc ggcgtgcagg cggtgatcgc cggcaatggc acggccaacg tctatgtcgg
     7021 ttccggtcag gcgctggtca cgggtttcga cgtgcagaag ctggaggtcg ggcccgatgc
     7081 ctataccggc gaggatctcg cggtgcgcta cgccggccag aggcagggca ccgacctcgc
     7141 gccctacctg accggcggca agatcggcgg ctacgtggcg tttcgcgacg gcatgctgag
     7201 ccgcgtgcag ggcgatgtcg gcctgttcgc cgccggcatc gccgatgccg tcaacagcca
     7261 gcagaccagg ggactcgacc tgcagggccg gctcggcgag gcgatgttca cgacctcggc
     7321 ggcgcgcgtg ttcgcccatg acagcaacga cggcgatgcg caggtcagcg tcacgatcgc
     7381 cagggtcccg gacctgcagc gttcggacta cgaactgcgc tacgccaacg gcaattattc
     7441 gctgctgcgc ctcagcgaca gccaggtcat cgccaccggc agcggcccgg gcctgaccgg
     7501 cgacgggctc gacatcgaca tcccgtccgg cacgccggcc aacggcgatc gttatctgat
     7561 ccagccgaca cggctcgccg cttcccagat cgaggtcgcc ctgatcggca ccgaccagat
     7621 cgccgctgcc gcgccgatcg cgaccgccgc ggcgcgcgcc aacatcggca ccggcagcat
     7681 cagcgccggc accgtgaaca ccttgccgac gaccgcgagc gatgtgctga aaccggtcga
     7741 gatccgcttc accagcgaga ccacgtacga cctcgtcgat gtcagcgatc cgaacaatcc
     7801 ggtgacgctg cagagcgcca gcgcgtacag cgcgagtgcc ggcgccacga tcggcgtcaa
     7861 cggctgggag gtgcgcgtct ccggccagcc gcagaacggt gatgtgttca cggtcggtcc
     7921 gaacacggcc ggctccggcg acaaccgcaa catgctggcg ctggccgggc tggccacgaa
     7981 gcgcgtgctg atcggcagca cggccgacct caagggcttc tacgccggcg tcatcggcga
     8041 catcggcacg cagtcgcagc gcatcagtac cgcggtgacg gcgcagacgg cgctgctgaa
     8101 tcaggcgaag caggcgcgtg aatcggcttc cggcgtcaa
//
LOCUS       DAOQIE010000175         8066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4594_length_8066_cov_7.975159, whole genome shotgun sequence.
ACCESSION   DAOQIE010000175 DAOQIE010000000
VERSION     DAOQIE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4594_length_8066_cov_7.975159"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..816
                     /locus_tag="PK144_11425"
     CDS             292..816
                     /locus_tag="PK144_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66385.1"
                     /translation="MTTPCDSRTCAPAGACVASCRQRLRRGVWLAGCLLVLGGCVEPA
                     YYGGSPYYEERNSYYESRPVYTQPDYYGGSYYRPTPRVQAYYYDRGYSHARRDRDWHD
                     HDRDRRGRDYRDDDRRSRNDGRGDEHREARRDQDRSRDSGDHRASSGRRTDLEWTDRM
                     RRAQGGNGETRPYD"
     gene            809..1132
                     /locus_tag="PK144_11430"
     CDS             809..1132
                     /locus_tag="PK144_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66386.1"
                     /translation="MTDAVRQPEVRRPALVRWGLAGILLALTGGCVAVAPDYGYGYGY
                     GYAPAYSPAPPAYSYAPAPVVVAPPVVVRPYYRPYYRDYRPYPRPYYRPYHNHGGWGH
                     GHRRY"
     gene            1113..1589
                     /locus_tag="PK144_11435"
     CDS             1113..1589
                     /locus_tag="PK144_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66387.1"
                     /translation="MAIAATERRTLRGRTGLLLALCCQAGAAAEPSPFTGLWADAPGL
                     HRLVLVDAPDGHLQAVLYGTLADGRRYVVSAGSVREADDGLWLDWPAPAAYRGPVAGA
                     DALAGHRAELPPAAGLAPGPSRWQAVRAAGGLRLRCLHGCAGWPAELHLTPMPAGR"
     gene            1821..2021
                     /locus_tag="PK144_11440"
     CDS             1821..2021
                     /locus_tag="PK144_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66388.1"
                     /translation="MCTFDSPFERCPVCQQIVLLDSTQKECAHEHSCVPGQVCPLGAY
                     FDGLKFQESAQERKVIAIQPLG"
     gene            complement(2221..2952)
                     /gene="tatC"
                     /locus_tag="PK144_11445"
                     /pseudo
     CDS             complement(2221..2952)
                     /gene="tatC"
                     /locus_tag="PK144_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
     gene            complement(2956..3378)
                     /gene="tatB"
                     /locus_tag="PK144_11450"
     CDS             complement(2956..3378)
                     /gene="tatB"
                     /locus_tag="PK144_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNF66389.1"
                     /translation="MFEIGFWELVLIGVVALVVIGPERLPGVARTVGRWVGRARQIVS
                     TVRSEVERELQIEEMKRTIAEHDTAEQFRQLHDQIRTLESELRSSVQSADPRTAATPE
                     PLAPPVETAAPLPAASSPPTAPAATPASPAPAAAAHRD"
     gene            complement(3394..3657)
                     /gene="tatA"
                     /locus_tag="PK144_11455"
     CDS             complement(3394..3657)
                     /gene="tatA"
                     /locus_tag="PK144_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNF66390.1"
                     /translation="MGFGSIWHWLIVLLIVLVVFGAKKLRNVGSDLGAAVKGFKDAMA
                     ENDNKPETPAQPAEPPKPAGSIEHGRVIEGQATREHDREPPKV"
     gene            complement(3743..4096)
                     /locus_tag="PK144_11460"
     CDS             complement(3743..4096)
                     /locus_tag="PK144_11460"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNF66391.1"
                     /translation="MTSSPAGALLRADDVLARLAAVLESRRGADPASSYVAKLYGKGL
                     DAILKKIGEEATETVLAAKDGDAQHLVYEIADLWFHTLVLLAHQNLGPEAVLAELDRR
                     FGLSGLAEKAARTPD"
     gene            complement(4093..4485)
                     /gene="hisI"
                     /locus_tag="PK144_11465"
     CDS             complement(4093..4485)
                     /gene="hisI"
                     /locus_tag="PK144_11465"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNF66392.1"
                     /translation="MSTAALDAIRWTADGLVPVIAQEAGSGRVLMFAWMNREALARTV
                     ATGEAVYWSRSRGRLWHKGEESGHVQKVRDLRLDCDADVLLLEVEQVGGIACHTGRHH
                     CFFQALDANGDWTAVEAVLKRPDEIYRS"
     gene            complement(4500..5273)
                     /gene="hisF"
                     /locus_tag="PK144_11470"
     CDS             complement(4500..5273)
                     /gene="hisF"
                     /locus_tag="PK144_11470"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018918913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNF66393.1"
                     /translation="MGLAKRIIPCLDVDAGRVVKGVNFVEIRDAGDPVEIARRYDAEG
                     ADEITFLDITASSDDRATMVHVVEQVAGQVFIPLTVGGGIRSVADVRRMLNAGADKVS
                     INTAAVSRPELVREAADYFGNQCIVVAIDAKRTSAAGEPLRWEVFTHGGRRRTGLDAI
                     EWAQRMAAFGAGELLLTSMDRDGTKIGFDLELTRAITEAVPVPVIASGGVGNLDHLAD
                     GVQLAGADAVLAASIFHFGEYTIGEAKRHMAARGIEVRL"
     gene            complement(5275..6012)
                     /gene="hisA"
                     /locus_tag="PK144_11475"
     CDS             complement(5275..6012)
                     /gene="hisA"
                     /locus_tag="PK144_11475"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNF66394.1"
                     /translation="MLLIPAIDLKDGKCVRLRQGRMEDSTVFSDDPVAMAGRWVQAGA
                     QRLHLVDLNGAFAGRPQNAEVVRRIADAFPDLPIQVGGGIRSEETVETYLNAGVRYVI
                     IGTKAVQEPHFVSDLCMTFPGHVIVGLDARDGKVAVDGWSKLSKHDVIDIAQRFERDG
                     VEAIVYTDIGRDGMMQGVNVEATVRLAQAVSIPVIASGGVTNLDDIKALCAVEGEGVA
                     GTILGRALYEGTLDLAEAQRYVDQRKT"
     gene            complement(6060..6713)
                     /gene="hisH"
                     /locus_tag="PK144_11480"
     CDS             complement(6060..6713)
                     /gene="hisH"
                     /locus_tag="PK144_11480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNF66395.1"
                     /translation="MSAVIAVIDYGMGNLHSVAKALEHVAPLARVRVTQRATDILEAD
                     RVVFPGQGAIGHCMQELARLDLVETVRRAAREKPFLGMCLGPQALMSHSDENGGIAAL
                     DVFAGDVHRFPEGLRDAHGLRLKIPHMGWSCVQQIRPHALWAGIEDGARFYFVHSYHL
                     APADPALVAGETEYGIRFATALARANVFAVQFHPEKSAAAGLQLLANFAAWDPDRTA"
     gene            complement(6746..7339)
                     /gene="hisB"
                     /locus_tag="PK144_11485"
     CDS             complement(6746..7339)
                     /gene="hisB"
                     /locus_tag="PK144_11485"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNF66396.1"
                     /translation="MSARTAEIRRDTLETQIRVAINLDGQGHGRFDTGVPFLEHMLDQ
                     VARHGLIDLDISCRGDLHIDAHHTVEDVGIAFGQALRDALGDRRGIRRYGHAYVPLDE
                     ALSRVVVDFSGRPGLEYFVEYPRARIGEFDVDLIREFFQGFVNHAQLTLHLDNLRGRN
                     AHHIAETLFKAFGRALRMAAEPDPRMQGIMPSTKGTL"
     gene            7510..>8066
                     /locus_tag="PK144_11490"
     CDS             7510..>8066
                     /locus_tag="PK144_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNF66397.1"
                     /translation="MNDCSPKPLDLAMLRLACQNCSLHELCLPVGIGLDDVGRLDQII
                     KRKRPLARADHIFRLGDEFRSVYAIRAGSVKTYTMTETGGEQIIGFYLPGEIIGLDAI
                     HNGAHPSSARTLETTSICEIPFAQLEDLAVAIPSLGRQLVRIMSREIHSDDELLTLLG
                     KKSAEERLASLLMSLSLRLQQRGFSA"
BASE COUNT         1213 a         2671 c         2891 g         1291 t
ORIGIN      
        1 gcccttcgag cagcgcggcg gtcgcagcgc cgatgccggt gcgatgaccg gtgacgatcg
       61 ccaccttgcc ggcaagaccg ggcaggcagg gggcgggcgg gggattcggc atgcgggcag
      121 gctccgggcg cgggcagggg cgcgagtatg tcgcaagcgg tgacggaccg ctgccgctgg
      181 ccctgctgcg ccatactgac cgtctgacgc acgcaaccac cggggcgtgt gccgtgtctg
      241 aatcctggcc ggtgttctcc cttccgtgcc cggcgctgcc gaggttcgat catgaccact
      301 ccctgcgatt cccggacgtg cgcgccagcc ggcgcgtgcg ttgcttcgtg tcgccagcgc
      361 ctgcggcgcg gcgtctggct ggccggctgc ctgctcgtgc tcggcggctg cgtcgagccg
      421 gcctactacg gcggctcgcc gtattacgaa gaaagaaatt cgtattatga aagcaggccg
      481 gtgtacacgc agccggatta ctacggcggc agctattacc ggccgactcc ccgggtccag
      541 gcgtattact acgaccgcgg atactcgcat gcgcggcggg atcgcgactg gcatgaccac
      601 gaccgtgacc ggcgcggtcg cgattaccgc gatgatgacc ggcgctcgcg caatgacggt
      661 cgcggcgatg agcatcgcga agcccgccgc gatcaggacc ggtcccggga ttccggcgat
      721 catcgggcct cttccggccg gcgtaccgat ttggaatgga cggaccgcat gcgcagggcc
      781 cagggtggta acggtgaaac acgtccttat gactgatgcc gtccggcagc ctgaagtccg
      841 gaggccggcc cttgtccgct gggggcttgc aggcatcctg ctggccctga caggcggctg
      901 cgtggcggtc gcgcccgatt acggctacgg ctacggctac ggctacgccc cggcctattc
      961 gccggcgccg ccggcctaca gctacgcccc cgcgcccgtg gtcgtcgcgc cgccggtggt
     1021 cgtgcggccg tactaccggc cttattaccg cgattaccgg ccttatcctc gcccttatta
     1081 ccggccttat cacaatcacg gcggctgggg gcatggccat cgccgctact gagcgccgga
     1141 ccctccgggg ccggacgggc ctgctgctcg cactctgctg ccaggcgggt gcggcagcag
     1201 agccgtcgcc gttcaccggc ctctgggccg atgcccccgg cctgcaccgg ctggtgctgg
     1261 tggacgctcc ggatggacac ctgcaggccg tgctctacgg cacgctggcc gatggccggc
     1321 gctacgtggt cagtgccgga tcggtccggg aagccgatga cggcctgtgg ctcgactggc
     1381 cggctccggc cgcctatcgc gggccggtcg ccggcgcgga tgcgctcgcc gggcaccggg
     1441 ccgaactgcc gccggcggcc ggcctcgcgc ccggacccag tcgctggcag gccgtgcgcg
     1501 cggccggcgg attgcggttg cgctgcctgc acggctgtgc cggctggccc gcggagctgc
     1561 atctgacacc gatgccggcc gggcgctgag cccgggttct cgcaggacga atttccagtt
     1621 cacgccgttt cggcaccgcc ggcattgatg tgcggcagcc ggggcgtgga cgcgattcgc
     1681 aatcctgatc ccggtcaatg aaaagcccgg cagattgtga gcaaatgtgg gcagacacgc
     1741 agatacaacg atagacaggg atgcgcggga tcggattttc gaatcacgaa ccacagagca
     1801 ccaatgagag gagagccatc atgtgtacct tcgactcgcc gtttgaacgc tgtccggtct
     1861 gccagcagat cgtgctgctc gactcgaccc agaaggaatg cgcacacgaa cacagctgcg
     1921 tgccgggaca ggtctgcccg ctcggcgcgt atttcgacgg cctgaagttt caggagagcg
     1981 cacaggagcg caaggtcatc gccatccagc cgctcggctg agacggcaag tcattcggga
     2041 gggggaaccc ggagctgacc gtacacggac gtgcggcagg caaaaaagcg gggcggcggc
     2101 aggatgccga ccgccccgac tccatctgga tgggttgcgg atggatgggt tacggatgga
     2161 tgggctgcgc gggccgggaa ggccggctca gtcgccttcc tcggcgatgg ccggcgtcgc
     2221 ggcccgccgg cgttcgatca gccgggcgat gtagacgccg atctcgaaca gcatccacat
     2281 cggcaccgcc agcagcgtct gcgagatcac gtccggcggg gtcagcaccg cgccgaccac
     2341 gaaggcaccg accaccacgt acgggcgctt ggcggcgagc tgatcggcgg tcatgacgcc
     2401 gaacagcacc agcacgacgg tcgcaaccgg cacctcgaag cagacgccga acgcgaagaa
     2461 gatcgtcagc acgaagtcga ggtagtggtt gatgtcggtc atcaccgcca cgccctgcgg
     2521 tgcggtggtg gtcaggaacg cgaagaacac cggcaggatc acccagtacg cgaaagccat
     2581 gccggcgtag aacagcgccg tgctggccgc gaccagcggc agcgcgtact gcttctcgcg
     2641 cggatacagg cccggcgcga cgaaggccca gacctggtac aggatccacg gcatcgccag
     2701 gaagatcgcc atgaccaggc agagcttcag tggcgtcagg aacggcgaga tcacgtcgat
     2761 cgcgatcatg ctgctcgatg ccggcatgtg cgcgatcagc ggctgcgcga actggctgta
     2821 gacgccgttg gcaaacggca gcaggcagag cagcacggcg aagacgccgg ccaccatgcg
     2881 cagcagccgg tcccgcagtt cgagcagatg cgtcaggatc ggctgttcgc tgtcgtggat
     2941 ctgctggctc acggttcagt ctcggtgcgc ggcggcggcc ggggcgggtg atgccggcgt
     3001 tgcggcgggg gcggtggggg gcgaactggc ggccggcagc ggtgcggcgg tttcgaccgg
     3061 tggcgcgagc ggctccggcg tggcggccgt gcgcgggtcg gcgctctgca cgctggagcg
     3121 cagttcgctt tccagcgtgc ggatctggtc gtgcagctgg cggaactgct cggccgtgtc
     3181 gtgctcggcg atcgtgcgct tcatctcctc gatctgcagt tcgcgctcga cctcggagcg
     3241 cacggtcgag acgatctgcc gcgcccgccc gacccagcgc ccgaccgtgc gcgcgacgcc
     3301 gggcagccgt tcggggccga tcacgaccag cgcgacgacg ccgatcagca ccagctccca
     3361 gaagccgatt tcgaacatcg tgcggctgcc ggctcagacc ttcggcggct cgcggtcgtg
     3421 ctcgcgggtc gcctgaccct cgatcacgcg accgtgctcg atgctgccgg ccggcttcgg
     3481 cggctcggcc ggctgcgccg gggtttccgg cttgttgtcg ttctcggcca tcgcgtcctt
     3541 gaagcccttg acggcggcgc cgaggtcgga gccgacgttg cgcagcttct tggcgccgaa
     3601 cacgaccagc acgatcagca gcacgatcag ccagtgccag atgcttccga aacccatggg
     3661 ggcagtcctc tcttctctct ttcagtggcc ggcgccgcgg gatgcgggcg ccggggcggg
     3721 cgcgcgggcc ggagcggggg tgtcagtccg gcgtacgcgc ggccttctcg gccagtcccg
     3781 acaggccgaa gcgccggtcg agttcggcga gcacggcctc gggaccgagg ttctggtgcg
     3841 cgagcagcac cagcgtgtgg aaccagaggt cggcaatctc gtagaccaga tgctgcgcat
     3901 ccccgtcctt ggcggcgagc acggtctcgg tggcttcctc gccgatcttc ttcaggatcg
     3961 cgtcgagtcc cttgccgtag agcttcgcga cgtaggagct ggccggatcg gcgcccctgc
     4021 ggctttcgag cacggcggcg aggcgcgcga gcacgtcgtc ggcgcgcagc agggcgccgg
     4081 cgggggagct ggtcatgagc ggtagatctc gtcggggcgc ttcaggaccg cttcgaccgc
     4141 ggtccagtcg ccattggcat cgagcgcctg gaagaagcag tgatgacggc cggtgtggca
     4201 ggcgatgccg ccgacctgct cgacttcgag cagcagcacg tcggcgtcgc agtcgaggcg
     4261 caggtcgcgg accttctgca catgcccgga ttcctcgccc ttgtgccaga gccggccgcg
     4321 cgagcgcgac cagtacacgg cctcgccggt ggcgacggtg cgcgcgagcg cctcgcggtt
     4381 catccacgcg aacatcagca cgcggccgct gccggcttcc tgggcgatga ccggcaccag
     4441 gccgtcggcg gtccagcgga tcgcatcgag ggcggcggtc gagatcgtgg ccggggcgct
     4501 cacagccgca cctcgatgcc gcgcgcggcc atgtggcgct tggcctcgcc gatcgtgtat
     4561 tcgccgaagt ggaagatgct ggcggcgagc acggcatcgg cgccggcgag ctgcacgccg
     4621 tcggcgagat gatcgaggtt gccgacgccg cccgaggcga tcaccggcac cggcaccgcc
     4681 tcggtgatgg cgcgtgtcag ttcgaggtcg aagccgatct tcgtgccgtc gcggtccatg
     4741 ctggtcagca gcagttcgcc ggcaccgaag gcggccatgc gctgcgccca ttcgatggcg
     4801 tcgaggccgg tgcgccggcg tccgccgtgc gtgaagactt cccagcgcag cggctcgccg
     4861 gcggcgctgg tgcgcttggc gtcgatcgcg acgacgatgc actggttgcc gaagtagtcg
     4921 gcggcctcgc gcaccagctc gggccggctg accgcggcgg tgttgatcga gaccttgtcg
     4981 gcgccggcgt tgagcatgcg ccgcacgtcg gcgaccgagc ggatgccgcc gccgacggtc
     5041 agcgggatga acacctgacc ggcgacctgc tcgacgacgt ggaccatggt cgcgcggtca
     5101 tcggaactgg cggtgatgtc gaggaaggtg atctcgtcgg caccctcggc gtcgtagcgg
     5161 cgcgcgatct cgaccggatc gccggcatcg cggatctcga cgaagttgac tcccttgacg
     5221 acgcgtccgg cgtccacgtc gaggcagggg atgatgcgtt tggcgaggcc catttcaggt
     5281 cttccgttgg tccacgtagc gctgcgcctc ggcgagatcg agcgtgccct cgtacagcgc
     5341 gcgcccgagg atggtgccgg cgacgccttc gccttcgacc gcgcacaggg ccttgatgtc
     5401 atcgagattg gtgacgccgc ccgaggcgat gaccgggatc gacacggcct gcgcgaggcg
     5461 cacggtggcc tcgacgttga cgccctgcat catgccgtcg cggccgatgt cggtgtagac
     5521 gatggcctcg acgccatcgc gttcgaagcg ctgcgcgatg tcgatcacgt cgtgcttcga
     5581 gagcttcgac cagccatcga cggcaacctt gccgtcgcgg gcatcgagtc cgacgatcac
     5641 gtgccccgga aaggtcatgc acaggtccga cacgaagtgc ggctcctgca cggccttggt
     5701 gccgatgatc acgtagcgca cgccggcgtt gaggtaggtc tcgacggttt cctcgctgcg
     5761 gatgccgccg ccgacctgga tcggcaggtc cggaaaggca tcggcgatgc ggcgcacgac
     5821 ctcggcgttc tgcggccggc cggcgaaggc gccgttgagg tcgacgaggt gcaggcgctg
     5881 cgcgccggcc tgcacccagc gcccggccat cgccaccgga tcgtcggaga acacggtgga
     5941 gtcctccatc cgtccctggc gcaggcggac gcacttgccg tctttcagat cgatggccgg
     6001 tatcagcaac atgggcgagg tcctctgtcc ccgggggtgc tagcgagcgg tggaagtcgt
     6061 caggccgtcc ggtcggggtc ccaggcggcg aagttggcga gcagctgcag tccggcggcc
     6121 gcgctcttct cgggatggaa ctgcacggcg aacacgttgg cgcgggccag ggcggtggcg
     6181 aagcggatgc cgtattcggt ctcgccggcg accagggccg gatcggccgg ggcgaggtgg
     6241 tagctgtgca cgaaatagaa gcgggcgccg tcctcgatgc cggcccagag cgcgtgcggg
     6301 cgtatctgct gcacgcagct ccagcccatg tgcggaatct tcagccgcag gccgtgcgca
     6361 tcccgcagcc cttccgggaa gcggtgcacg tcgcctgcga acacgtcgag ggcggcgatg
     6421 ccgccgttct cgtcgctgtg cgacatcagc gcctgcggcc cgaggcacat gccgaggaag
     6481 ggtttctcgc gggcggcgcg gcgcacggtt tcgaccaggt cgagccgggc gagctcctgc
     6541 atgcagtggc cgatcgcgcc ctgacccggg aagacgacgc ggtcggcctc caggatgtcg
     6601 gtggcgcgct gcgtgacgcg cacgcgtgca agcggcgcaa cgtgttcgag ggccttggca
     6661 accgagtgca ggttgcccat tccgtagtcg atgacggcga tgacggcaga cattcagcga
     6721 ttcccggcga cggcggtggt cggcgtcaga gcgtgccctt ggtcgagggc atgattccct
     6781 gcatgcgcgg atcgggttct gcggccatgc gcagggcccg tccgaacgcc ttgaacaggg
     6841 tttcggcgat gtggtgggcg ttgcgcccgc gcaggttgtc gaggtgcagc gtcagctggg
     6901 cgtggttgac gaagccctgg aagaattcgc ggatcaggtc gacgtcgaac tcgccgatgc
     6961 gcgcccgcgg gtactcgacg aagtattcga gcccgggccg gccggagaaa tcgacgacga
     7021 cgcgcgacag cgcctcgtcg agcggcacat aggcgtggcc gtagcggcgg atgccgcggc
     7081 ggtcgccgag cgcatcgcgc agggcctgac cgaaggcgat gccgacatcc tcgaccgtgt
     7141 ggtgcgcgtc gatgtgcagg tcgccgcggc agctgatgtc gaggtcgatc aggccgtggc
     7201 gggcgacctg gtcgagcatg tgttcgagaa acggcacgcc ggtgtcgaag cggccgtggc
     7261 cctggccgtc gaggttgatc gcgacgcgga tctgcgtttc gagcgtgtcg cgccggatct
     7321 cggcagttcg tgcggacatg tgcggtgggg ttcccggctg gcgcatcttg caaatcgtta
     7381 gcatatacgg gtgtgccggc cactggaaac gctgcggaca tgcgcatttg cgcctccggt
     7441 ggtttcgttg cgtcaggtca atgaatctgg tgcaatgaag tgtcaacgcc tcgtgccggt
     7501 atgccgccga tgaacgactg ttccccgaag ccgctcgatc tcgccatgct gcgcctggcc
     7561 tgccagaact gcagtctcca tgagctgtgc ctgccggtcg gcatcggcct cgacgatgtc
     7621 ggccgcctcg accagatcat caagcgcaag cgcccgctgg cgcgcgccga ccacatcttc
     7681 cggctcggcg acgagttccg ctcggtctac gcgatccgcg ccggctcggt gaagacctac
     7741 acgatgaccg agaccggcgg cgagcagatc atcggcttct acctgcccgg cgagatcatc
     7801 ggcctcgacg ccatccacaa cggcgcgcat ccgtcgagcg cccgcacgct cgaaaccacc
     7861 agcatctgcg aaatcccgtt cgcgcagctc gaagacctcg cggtcgcgat cccctcgctc
     7921 gggcgccagc tcgtgcgcat catgagccgc gagatccact ccgacgacga actgctgacc
     7981 ctgctcggca agaagtcggc cgaggagcgc ctcgcctcgc tgctgatgag cctgtcgctg
     8041 cgcctgcagc agcgcggctt ctcggc
//
LOCUS       DAOQIE010000176         8048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4612_length_8048_cov_7.871387, whole genome shotgun sequence.
ACCESSION   DAOQIE010000176 DAOQIE010000000
VERSION     DAOQIE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4612_length_8048_cov_7.871387"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(450..1649)
                     /gene="tyrS"
                     /locus_tag="PK144_11495"
     CDS             complement(450..1649)
                     /gene="tyrS"
                     /locus_tag="PK144_11495"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNF66398.1"
                     /translation="MATVAESLDIIKRGAQELLIESELIERLGSGRPLRVKAGFDPTA
                     PDLHLGHTVLINKLRQFQELGHEVLFLIGDFTGMIGDPTGKNVTRKPLSREDVLANAE
                     TYREQVFRILDPARTQVLFNSAWYDTMKPADFIQLAARHTVARMLERDDFSKRYAAGQ
                     PIAIHEFLYPLVQGYDSVAMRADVELGGTDQKFNLLVGRELQKDYGQTPQIVLTMPIL
                     EGTDGVQKMSKSLGNYIGIRDAADDMFGKLMSISDTLMWRYLELLSFRPMSEIEAWQR
                     EVEAGLNPRDVKFRLGAELVDRFHGSGAGERAVAAFVERFQKGALPDELPEVTVQAVN
                     GALPIANLLKEAGLVKSTSDALRMLAQGAVRLDGERVSDRGLSLQAGSTVVAQVGKRA
                     VARVTIA"
     gene            1776..2894
                     /locus_tag="PK144_11500"
     CDS             1776..2894
                     /locus_tag="PK144_11500"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNF66399.1"
                     /translation="MRNDDAPLIGLMSGTSMDAVDAVLVRFEAGRPRTLARHALPLPA
                     ELRSALLGLAAPGHDELDRAAQLDNRVARLFAQAVRELLELGGLRAGQVRAIGSHGQT
                     IRHAPSAAEPYTLQIGNPALLAELTGITVVADFRRRDLAAGGQGAPLAPAFHAAFFAR
                     DAADTAVINIGGIANLTHLPAGGGPVTGFDTGPGNLLLNAWTERYLGEPMDRDGAWSA
                     GGEVNAALLDRLLADDYFSAPPPKSTGREHFHLDWLDTHCRALGRALPPRSVAATLVE
                     LSARTIADACHAHAPVADAWLICGGGVHNRLLMARLSALAGPVPVRSTAACGVDPDDL
                     EALGFAWLAQRTLSGLPGNLPEVTGARGPRILGGIYPA"
     gene            complement(2882..4066)
                     /gene="nhaA"
                     /locus_tag="PK144_11505"
     CDS             complement(2882..4066)
                     /gene="nhaA"
                     /locus_tag="PK144_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNF66400.1"
                     /translation="MLSALRHFLKLEAASGILLFLAAVLAMIVANSPLAPWYGQFLDL
                     PVAVRIGPLEIAKPLLLWINDGLMAVFFLLVGLELKREVLEGELADPRQVALPAIAAI
                     GGMIVPALVYCAFNSGDPVALRGWAIPAATDIAFALGILALLGSRVPLALKVFLVSIA
                     IFDDLGAIVIIALFYTQDLSMLALGISLACLPLLLLLNRCNVTTFTPYMLIGLLMWTA
                     LLKSGIHATLAGVVLALFVPMRDRKAPESSPLRRLEHDLHTGVAFGILPLFAFANAGI
                     SFAGVGLQALTHPVALGVAGGLLIGKPLGVYGASLLGLKLGIGRLPESVDRRALFGVA
                     VLCGIGFTMSLFIGSLAFPEDAHAELFAERFGIVAGSLVSGLIGFVLLRRALPAAAVQ
                     AG"
     gene            complement(4249..4587)
                     /gene="erpA"
                     /locus_tag="PK144_11510"
     CDS             complement(4249..4587)
                     /gene="erpA"
                     /locus_tag="PK144_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNF66401.1"
                     /translation="MSEESLLVFTDSAATKVRSLIEEENNPALMLRVFVSGGGCSGFQ
                     YGFTFDEEVQDGDTMVENCGVRLLVDPMSYQYLVGAEIDYTEGLEGAQFVIRNPNATT
                     TCGCGSSFTV"
     gene            complement(4714..5751)
                     /gene="argC"
                     /locus_tag="PK144_11515"
     CDS             complement(4714..5751)
                     /gene="argC"
                     /locus_tag="PK144_11515"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNF66402.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAAHPGVELTAITSRSEKGLKVAD
                     MFPNLRGRVDLAFVEPSPAVLEACDAVFFATPNGTAMKMVPELLAAGVRVIDLAADFR
                     LGDPAEWQQWYGMAHACPELLAEAVYGLPEVNREAIRAARLVANPGCYPTAVQLGFLP
                     LIEAGLIDDAQLIADAKSGVSGAGRKAEVHTLLCEAGENFKAYGVGGHRHLPEIRQGL
                     ARAAGHAVGLTFVPHLTPMIRGIHATLYGRLKSGAGAGDLQALYESRYASETFVDVLP
                     AGSHPETRSVRGANTCRIAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNLNLMFGQPE
                     TAGIDGIALLP"
     gene            6007..7284
                     /locus_tag="PK144_11520"
     CDS             6007..7284
                     /locus_tag="PK144_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNF66403.1"
                     /translation="MVRAHDPRPGLFLLGCAALCGVLSGLVMVAFRLLIDGAQMRLLG
                     AGRLDDFAALAPLTRIALAGAGGAALGLLWARLPAATRAVGVAHVLERCAYHGARLPG
                     INALLQFLGAASCLICGHSVGREGPAIHIGATAGSLLAERAALSLDARRLLVACGTAG
                     GIAAAFNTPLAGVAFAVEVLLGLSALPNLTPVLLAAVVATAISRGFYGDHALLPAAPP
                     GFDALAQLPGLALQGALTGLLAVAFVQTLRTVAGRSQAAGPALRLTLGGLACGVIGAG
                     WPEVMGLGYGTARAAIDGTLTALTLAPLLAAKFAATVLGLGLGLPGGLIGPSLVLGAL
                     AGGLSGHWLPGADPGLCALLGMGAMMGATLNAPLAALVALLELTQDPRLILPAMLTIA
                     AADLVSHRVFGLPSVFVAQLQMRGIDWPPRERP"
     gene            7346..8020
                     /locus_tag="PK144_11525"
     CDS             7346..8020
                     /locus_tag="PK144_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNF66404.1"
                     /translation="MTAVDQLALALGAGWASGLNLYAAVLALGYFNATGDLLLPPELQ
                     ILSDPLVMLAAGLMYCVEFFADKTPGVDSGWDALHTFLRIPAGAVLAAGAVGDVSPAL
                     QIAAGLVGGTLAAGTHFTKAGARVLINTSPEPFSNWAASVTEDVAVFAAIWTAVHHPA
                     LWLALLALSVALIVWLLPRLWRGIRRVLSSLARWFGFGSGRPPASPIPPAGPPPAPPR
                     LPPPAP"
BASE COUNT         1167 a         2873 c         2778 g         1230 t
ORIGIN      
        1 tcaaccatta ccgccagtca atcattcccg ccttccagcg aggccgcgca ttctaacgtt
       61 acttagcgat gcgtcaagcc ctgaattcgc ttcactgctt tcgctgatta ccggaaactg
      121 cattccgact ttcagcgagg cgcgcattct acacgccgtt tttcgttctg caagcggtta
      181 ttttcaaacc gctttcgctt tccggattcc agcatcgcag cacaaccgca attaccggaa
      241 cccctcagca aggccgcgca ttctacgaat cagatcgctt tcgtcaagca ccggaatcag
      301 cgaatccact gaatccagaa accgccccga gcctgattgt ttcgagaatg tcacgcttcg
      361 cgattgcccg ccggatggcg gccggtcgcg aagagcggcg cattctacgg atcattgccg
      421 ggccgtcaag cccgcgtgaa ggttttattt caggcgatag tcacccgggc gacggcgcgc
      481 ttgccgacct gggcgacgac ggtactgccg gcctgcaggc tcagcccgcg gtcgctgacg
      541 cgttcaccgt cgaggcgcac cgcgccctgg gcgagcatgc gcagcgcgtc cgaggtgctc
      601 ttcacgaggc cggcctcctt gagcaggttg gcgatcggca gcgcgccgtt gacggcctgc
      661 acggtgacct cgggcagctc gtccggcagc gcacccttct ggaagcgttc gacgaaggcc
      721 gcgaccgcgc gctcgccggc accgctgccg tggaagcgat cgaccagttc ggcgccgagg
      781 cggaacttca cgtcgcgcgg gttcaggccg gcttcgacct cgcgctgcca ggcctcgatc
      841 tcgctcatcg gccggaacga cagcagttcg aggtagcgcc acatcagcgt gtcggaaatc
      901 gacatgagct tgccgaacat gtcgtcggcg gcatcgcgga tgccgatgta gttgccgagc
      961 gacttcgaca tcttctgcac gccgtccgtg ccttcgagga tcggcatcgt cagcacgatc
     1021 tgcggtgtct ggccgtagtc cttctgcagt tcgcggccga ccagcaggtt gaacttctgg
     1081 tcggtgccgc cgagttcgac atcggcgcgc atcgccaccg agtcgtagcc ctgcaccagc
     1141 ggatacagga attcatggat cgcgatcggc tggccggcgg cgtagcgctt gctgaagtcg
     1201 tcacgctcca gcatgcgtgc gaccgtgtgc cgcgcggcga gctggatgaa gtcggccggc
     1261 ttcatcgtgt cgtaccaggc cgagttgaac agcacctgcg tgcgcgcggg gtcgaggatg
     1321 cggaacacct gctcgcggta ggtctcggcg ttggcgagca cgtcctcgcg cgacaggggc
     1381 ttgcgcgtga cgttcttgcc ggtggggtca ccgatcatgc cggtgaagtc gccgatcagg
     1441 aacagcacct cgtggccgag ttcctggaac tggcgcagct tgttgatcag cacggtgtgg
     1501 ccgaggtgca ggtccggcgc ggtcgggtcg aaaccggcct tgacgcgcag cggccggccg
     1561 ctgccgagcc gctcgatcag ctccgactcg atcagcagct cctgtgcgcc gcgcttgatg
     1621 atgtccagcg attccgccac cgtcgccatc gtgtttcgtg ctctcgaccg tctcatgcag
     1681 ggggcgcgca ggataccgga agcgaccggc gcgcgcgaac gcatggcgga tcagacactt
     1741 ccgctgccca ccttgccttg tcaggaaacc tccggatgcg caacgacgac gccccgctca
     1801 tcggcctgat gtccggcacc agcatggatg ccgtcgatgc cgtgctcgtg cgcttcgagg
     1861 ccggccggcc gcggacgctg gcgcgccacg cactgccgct gccggcggag ctgcggtcgg
     1921 cgctgctcgg gctggccgcg cccggccatg acgagctcga ccgcgccgcg cagctcgaca
     1981 accgcgtcgc gcggctgttc gcgcaggccg tgcgcgagct gctcgaactc ggcggcctgc
     2041 gcgccgggca ggtgcgcgcg atcggctcgc acggccagac catccgccac gccccgtcgg
     2101 ccgccgaacc gtacacgctg cagatcggca atccggcgct gctcgccgag ctgaccggca
     2161 tcaccgtcgt cgccgacttc cgccgccgcg acctcgccgc cggcggtcag ggcgcgccgc
     2221 tggcgccggc cttccatgcg gcgttctttg cgcgcgacgc ggccgatacc gcggtcatca
     2281 acatcggcgg catcgccaac ctcacgcacc tgccggccgg cggcgggccg gtgaccggct
     2341 tcgataccgg ccccggcaac ctgctgctga acgcctggac cgagcgttac ctcggcgagc
     2401 cgatggaccg cgacggcgcc tggtcggccg gcggcgaggt caacgccgcg ctgcttgatc
     2461 gcctgctcgc cgatgactat ttctcggcgc cgccgccgaa gagcaccggc cgcgagcatt
     2521 ttcatctcga ctggctggat acgcactgca gggcgctggg ccgggcgctg ccgccgcgca
     2581 gcgtcgcggc aacgctggtc gagctgagcg cgcgcacgat cgccgatgcc tgccatgcgc
     2641 atgcccccgt ggccgatgcc tggctgatct gcggcggcgg cgtgcacaac cgcctgctga
     2701 tggcgcggct ttcggccctc gccggcccgg tgccggtgcg ctcgaccgcg gcctgcggcg
     2761 tcgatccgga cgacctcgaa gcgctcggct tcgcgtggct cgcgcagcgc acgctgtccg
     2821 gactgcccgg caacctgccc gaggtcaccg gggcgcgcgg gccgcggatc ctcggcggga
     2881 tctatccggc ctgaaccgcc gccgccggca gcgcccggcg cagcagcacg aagccgatca
     2941 ggcccgagac cagcgaaccg gcgacgatgc cgaagcgctc ggcgaacagt tcggcgtgcg
     3001 catcctccgg gaaggccagc gagccgatga acaggctcat cgtgaagccg atgccgcaga
     3061 gcaccgcgac gccgaacagc gcccgccggt cgacgctctc gggcaggcgg ccgatgccga
     3121 gcttcagccc gagcaggctg gcgccgtaga cgccgagcgg cttgccgatc agcaggccgc
     3181 cggcaacacc gagcgcaacc gggtgcgtca gcgcctgcag gccgacgccg gcgaacgaga
     3241 tgccggcgtt tgcgaacgcg aacagcggca ggatgccgaa ggcgaccccg gtgtgcaggt
     3301 cgtgttcgag ccggcgcagc ggcgaggact cgggcgcctt gcgatcgcgc atcggcacga
     3361 acagcgccag caccacgccg gcgagcgtcg cgtggatgcc ggatttcagc agcgccgtcc
     3421 acatcagcag cccgatcagc atgtagggcg tgaaggtcgt gacattgcag cggttcagca
     3481 gcagcagcag cggcaggcac gcgagcgaga tgccgagcgc cagcatcgac agatcctgcg
     3541 tgtagaacag cgcaatgatc acgatcgcac cgagatcgtc gaagatcgcg atcgacacca
     3601 ggaacacctt cagcgccagc ggcacgcgcg agccgagcag cgccaggatg ccgagcgcga
     3661 aggcgatgtc ggtcgccgcc gggatcgccc agcccctgag cgccaccgga tcgccggagt
     3721 tgaacgcgca gtagaccagc gccggcacga tcatgccgcc gatcgccgca atcgccggca
     3781 gcgcgacctg acgcggatcg gcgagttcgc cttcgagcac ctcgcgtttg agttccagtc
     3841 cgaccagcag gaagaacacg gccatcaggc cgtcgttgat ccacagcagc agcggcttgg
     3901 cgatctccag cggcccgatg cgcacggcca ccggcaggtc gaggaattgc ccgtaccagg
     3961 gcgcaagcgg cgaattggcg acgatcatcg ccagcaccgc ggcaaggaac agcaggatgc
     4021 cgctggcggc ttcgagtttc aggaagtgtc tgagggcact gagcatcggg cggcgtctcc
     4081 ggtcgggcgg cggcgggccg tgcggcctgc cgcggcgggc aagggaaggg cgcagtcggc
     4141 ccgaacctta gcacgcccgc ccgcgccgct tgccggaaac gacgaagagg ccttgcggcc
     4201 tcttcgtggg aagacgaaaa acggggaagg acgaaaaacc gcgcgtgctc agaccgtgaa
     4261 cgacgatccg cagccgcagg tggtcgtggc gttcgggttg cggatcacga actgcgcgcc
     4321 ttcgaggccc tcggtgtagt cgatctcggc gccgaccaga tactggtagc tcatcggatc
     4381 gacgagcagg cgtacgccgc agttctcgac catcgtgtcg ccgtcctgga cttcctcgtc
     4441 gaacgtgaag ccgtactgga agcccgaaca gccgccgccg ctgacgaaca cgcgcagcat
     4501 cagggccgga ttgttttctt cctcgatcag cgagcggacc ttggtcgccg ccgagtcggt
     4561 gaacacgagc agggattctt cggacatggc cgggtactcc tcgcgcaggc tgtgcgctgc
     4621 gaaaaatcgg gaatgcaccg gattatgggg ttcccgagca aagcgatcaa caatacccga
     4681 tccgcacgcc ggcgggccag gggtgccttg tggtcacggc agcagcgcga tgccgtcgat
     4741 gccggcggtt tcgggctggc cgaacatcag gttcaggttc tgcacggcct gaccggccgc
     4801 gcccttgacc agattgtcga tgaccgacag cacgacgacg gtgtcgccgc cctgcggccg
     4861 gtgcacggcg atccggcagg tgttggcgcc gcgcaccgag cgcgtctccg ggtgcgagcc
     4921 ggccggcagc acgtcgacga aggtctcgct cgcgtagcgg gactcgtaca gcgcctgcag
     4981 atcgccggca ccggcaccgg atttcagccg cccgtacagc gtcgcgtgga tgccgcggat
     5041 catcggcgtc aggtgcggca cgaaggtcag gccgacggcg tggccggcgg cgcgcgcgag
     5101 gccctggcgg atctcgggca ggtggcggtg gccgccgacg ccgtaggcct tgaagttctc
     5161 gccggcctcg cagagcagcg tgtgcacctc ggccttgcgc ccggcgccgg acacgccgga
     5221 cttggcatcg gcgatcagct gcgcatcgtc gatcagcccg gcctcgatca gcggcaggaa
     5281 gccaagctgc accgcggtcg gatagcagcc ggggttggcc accagccgtg ctgcgcggat
     5341 cgcctcgcgg ttgacctcgg gcaggccgta gacggcctcg gccagcagct cggggcaggc
     5401 gtgcgccatg ccgtaccact gctgccattc ggccggatcc ccgaggcgga agtcggctgc
     5461 gaggtcgatg acccggacgc cggccgcgag cagctccggc accatcttca tcgccgtgcc
     5521 gttcggcgtc gcgaagaaca ccgcgtcaca ggcttcgagc acggccggcg acggttcgac
     5581 gaaggccaga tcgacgcgcc cgcgcaggtt cgggaacatg tcggcgacct tcaggccttt
     5641 ttccgatcgc gaggtgatcg cggtcagctc gaccccggga tgcgcggcga gcagccgcag
     5701 aagttcgacg ccggtatacc cggtgccgcc gacgatgccg accttgatca tggcctgcca
     5761 ctccgtaacc gaaacattca gtagatgaag ccgggaggaa tccgggagga atccggaacc
     5821 ggtgcgtgcc ggaagccaca cccgctcacc ggtcgcgacg cccgcaggcc gccgtcaccg
     5881 ccccgcccgg cccggtggcc ggagcaaaaa gggcggcaat cataacgcca tgccgttacg
     5941 cgcgcgtatc cggcgcgcac cgtggcatgc tgcgcggcca tgccgaatcc gagccccccg
     6001 cccgccgtgg tacgtgcgca cgatccgcgg ccgggcctgt tcctgctcgg ctgcgcggcg
     6061 ctctgcggcg tgctgtcggg gctggtgatg gtcgccttcc ggctgctgat cgacggcgcc
     6121 cagatgcgac tgctcggcgc cggccggctc gacgacttcg ctgcgctggc gccactgacg
     6181 cgcatcgcgc tcgccggggc cggtggcgcc gcactcggcc tgctgtgggc acggttgccg
     6241 gcagcgacgc gggctgtcgg tgtcgcgcac gtgctggagc gctgcgcata ccacggcgcc
     6301 cggctgccgg ggatcaacgc gctgctgcag ttcctcgggg ccgcgagctg cctgatctgc
     6361 gggcattcgg tcggccgcga ggggccggcg atccacatcg gcgccaccgc cggcagcctg
     6421 ctcgccgagc gcgcggcgct gtcgctcgat gcccgccggc tgctggtcgc ctgcggcacg
     6481 gccggcggca tcgccgcggc cttcaacacg ccgctcgccg gcgtcgcctt cgccgtcgag
     6541 gtgctgctcg gcctgtcggc gctgccgaac ctgacgccgg tactgcttgc cgcggtcgtg
     6601 gcgacggcga tctcgcgcgg cttctacggc gatcacgcgc tgctgcccgc ggccccgccg
     6661 ggcttcgatg ccctcgcgca gcttccgggg ctcgcgctgc agggcgcgct gaccggcctg
     6721 ctggccgtgg ccttcgtgca gacgctgcgc acggtcgccg gccgctcgca agcggccggg
     6781 ccggcgctgc gcctgacgct cggcgggctg gcctgcggcg tgatcggcgc cggctggccg
     6841 gaggtcatgg gtctcggcta cggcacggcg cgcgcggcga tcgacggcac gctgacggcg
     6901 ctgacgctgg cgccgctgct cgccgccaag tttgcggcga ccgtgctcgg tctcggactc
     6961 ggcctgcccg gcgggctgat cggcccgtcg ctggtgctcg gtgcgctcgc cggcggcctg
     7021 agcggccatt ggctgccggg cgccgatccg gggctgtgtg cgctgctcgg catgggcgcg
     7081 atgatgggcg cgacgctgaa cgcaccgctg gcagccctgg tcgcactgct cgaactgacg
     7141 caggacccgc ggctgatcct gccggcgatg ctgaccatcg ccgcggccga tctggtctcg
     7201 caccgcgtgt tcggactgcc gtcggtgttc gtcgcccagc tgcagatgcg cggcatcgac
     7261 tggccgccgc gcgagcggcc ctgaggccgc acggccggcc gcccgctttc cccaccctcc
     7321 gcctgccccg caggaggtac gtcacatgac cgccgtcgat cagctcgccc tcgccctcgg
     7381 cgccggctgg gcttccggcc tcaacctgta tgccgcggtg ctcgcgctcg gctacttcaa
     7441 cgccaccggc gacctgctgc tgccgccgga gctgcagatc ctcagcgacc cgctggtgat
     7501 gctcgcggcc gggctcatgt actgcgtcga gttcttcgcc gacaagacgc ccggcgtcga
     7561 cagcggctgg gacgcgctgc acaccttcct ccgcatcccg gccggtgccg tgctcgccgc
     7621 cggcgccgtc ggcgacgtgt cgccggccct gcagatcgcc gccggcctgg tcggcggcac
     7681 gctcgccgcc ggcacgcact tcacgaaggc cggcgcgcgc gtgctgatca acacctcgcc
     7741 ggagccgttc agcaactggg cggcctcggt gaccgaggat gtcgccgtgt tcgcggcgat
     7801 ctggaccgcc gtgcatcacc cggcgctgtg gcttgcgctg ctcgcgctgt cggtggcgct
     7861 gatcgtctgg ctgctgccgc ggctctggag gggtatccgg cgcgtgctat cctcgctcgc
     7921 ccgctggttc ggcttcggca gcggccggcc accggcatcg ccgatcccgc ccgccggccc
     7981 gccgccggct cccccgcggc tgccgccgcc tgctccctga tcacccgcgc catgaaactc
     8041 accgacaa
//
LOCUS       DAOQIE010000177         8010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4640_length_8010_cov_8.033438, whole genome shotgun sequence.
ACCESSION   DAOQIE010000177 DAOQIE010000000
VERSION     DAOQIE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_8010_cov_8.033438"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="PK144_11530"
     CDS             complement(<1..1773)
                     /locus_tag="PK144_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNF66405.1"
                     /translation="MSDLAKIVVILAAAVLAVPLAKRLGLGAVIGYLLAGVLIGPWGL
                     ALIREHEEILHVGELGVVLLLFIVGLELQPSRLWALRRPVFGLGGLQVLLTGGAVMAV
                     MQWLGWHPGTAFVIGFGLSLSSTAFVLQLLGERGELTARHGRDAFAVLLMQDVAVIPM
                     LAILPLFAPSERFEPGEDLQKALVAGLAIAGVVLAGRYLLPRLFRLVAAAHSHEIATA
                     AALLVVAATALGFTHIGLSMSLGAFLVGMLLSDSEFRHELEANIEPFKGLLLGLFFVA
                     VGLSANLGLLTKEPGVLLALTAALIAVKALLLFLIARLFGTAPRPACKVAVLLAQGGE
                     FAFVLFAVARDLALLTTRQQDELNLAVTLSMAASPMLVRLTDSLAERLAERRRRRSPA
                     QAEPIAAAPVLIAGYGPFGQTVARYLRVHGIPCTVLETDLKRIELGRALGQTVHFGDA
                     SRIELLRAASAGDAHVLVIAVDDGETSLRIARTVRHHFPRLRTISRACDVRHTLALME
                     LGVGVVTSDTYLSALEMSEHVAVALGVPAGQAHTDVLHFRAHDQRNLDRLRAVHHDEA
                     RMLDTARAIIAEMEEVFRERRHTPA"
     gene            complement(1849..2166)
                     /locus_tag="PK144_11535"
     CDS             complement(1849..2166)
                     /locus_tag="PK144_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66406.1"
                     /translation="MLDFAAVEALLPEVAARLRAEFEDASEQSDAELHAFLRPVLRHA
                     ALHGFADADTGFVYAACAWLTGEDFASAFETPKTILAGSAPVADKAAALEDWLTGLID
                     PAD"
     gene            2370..2834
                     /locus_tag="PK144_11540"
     CDS             2370..2834
                     /locus_tag="PK144_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HNF66407.1"
                     /translation="MNRPATRSVHLAGSAALAVLLIAGGLAAPRAHADPRPHTVITEE
                     VRQEVTVSRHSVGPATTPPPAPTVTEMTPPPPPGAVVAADVPPPPPRVEQPVGQPAPE
                     YRWVPGHWSWENGAWAWTPGTWEQPPQQSAEWVPGRWEQRPDGWLWIPGYWS"
     gene            complement(2981..3775)
                     /locus_tag="PK144_11545"
     CDS             complement(2981..3775)
                     /locus_tag="PK144_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF66408.1"
                     /translation="MRLDAGCELEFEILEPTPMLLMLRPRSGEGQWVEAERYALQPRV
                     PASEYADAWGNLCQRIVARPGRLHISTQVRVDTDDTIALAPGAPRTPVDQLPVEVIGF
                     LLASRYAESDALADQARDVTAGATPGYDQVEAIRAWIHRSFEYRYGVSNESTSARDTI
                     RMRKGVCRDFAHVGIALTRALGIPARYVAGYLHELEPMDLHAWFEAYVDGRWYTFDAT
                     QAGPRGGRVIIGYGRDALDVALATPFGDTKLDNMQAWVRAVPEAAG"
     gene            complement(3876..4712)
                     /locus_tag="PK144_11550"
     CDS             complement(3876..4712)
                     /locus_tag="PK144_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF66409.1"
                     /translation="MSFRPSLPGLRAGLCALLLSVLSLPVQAAERFITVASTTSTEQS
                     GLFGYLLPKFTGKTGIEVRVVAVGTGQAIKIAQQGDADVLFVHHQPSEEKFVAEGYGV
                     QRFPVMYNDFIVVGPGTDPAAIEGGHDVVAAFRAIAGKQAPFASRGDDSGTHKAEQAL
                     WKAAGIDVKAASGSWYRETGSGMGPTLNTAAGLPAYALTDRGTWLSFRNRGPLKIVVE
                     GDKRLFNQYGILLVSPAKFPHVRQADAQAFIDWVISPEGQQAIAGYRINGEQLFFPDY
                     GR"
     gene            complement(4791..5252)
                     /locus_tag="PK144_11555"
     CDS             complement(4791..5252)
                     /locus_tag="PK144_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNF66410.1"
                     /translation="MSSASSTLPRPPLKVSAGNQIHGHVVTVERGAVNAEVLIDIGGG
                     ASMVAIVTDESIDALTLRPGREVYALVQASSVIFTTERMPFVSARNALWGVVDALRTG
                     AVNDEISMRLDAGQRVTATLTHTSVHELGLAPGRRACALIKASSVLLAVPA"
     gene            5365..6189
                     /locus_tag="PK144_11560"
     CDS             5365..6189
                     /locus_tag="PK144_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNF66411.1"
                     /translation="MSPIDPPSPADIATQLAFFARDGQTLLKPERVALLEHIQHSGSI
                     SAAARAIPMSYKAAWDAIDAMNNLAPQTLVERVAGGQRGGGSRLTEYGARIVARYRAI
                     ERMQRQLIDLIAMDDPQMLGDLPGLGLLFGLRTTARNLFSGRVARVAAGAVSSEVVID
                     IGAQDRLVAIVGRQDVEALRLVPGAAVLALVQATQVMLALAEAGLRTSARNRLDGTVL
                     CIGPGAVNAEVQIALAGGKRLTAMMTMESLQALGLEPGQPCTAWIKASSIVLAAIG"
     gene            6509..>8010
                     /locus_tag="PK144_11565"
     CDS             6509..>8010
                     /locus_tag="PK144_11565"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF66412.1"
                     /translation="MIYAKPGQPGSKVTFKSRYANFIGGEWVPPVEGRYFENVSPVDG
                     KVFCEIPRSSPADIELALDAAHRAKDAWGKASPTERSNTLLKIADRMEANQEMLAVAE
                     TWDNGKPIRESLAADTALAIDHFRYFAGCIRAQEGSIGEIDHDTVAYHFHEPLGVVGQ
                     IIPWNFPLLMAAWKIAPALGAGNCIVLKPAEQTPVSILVLAELIQDILPPGTLNIVNG
                     FGREVGQALATSKRIAKIAFTGSTATGRLVMQYATENIIPVTLELGGKSPNIFFDDVM
                     AKDDSFREKALEGFAMFALNQGEVCTCPSRALVQESIADEFLALAVERVSRIKQGDPL
                     DTDTMVGAQASADQVEKIMSYIDIGRQEGAQCLIGGERNQLGDSLGRGYYIKPTVFKG
                     NNKMRIFQEEIFGPVLSVTTFKDEAEALEIANDTIFGLGSGVWTRDGVRAYRMGRGIQ
                     AGRVWTNCYHHYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNLLVCYSQ"
BASE COUNT         1279 a         2825 c         2768 g         1138 t
ORIGIN      
        1 ggccggcgtg tgccggcgct cgcggaagac ctcttccatc tcggcgatga tcgcgcgcgc
       61 ggtgtcgagc atgcgtgcct cgtcgtgatg cacggcgcgc agccggtcga ggttgcgctg
      121 gtcgtgcgcg cgaaagtgca gcacgtcggt gtgtgcctgc ccggccggca cgccgagcgc
      181 gaccgcgacg tgctcgctca tctccagcgc cgacaggtag gtgtcgctgg tgacgacgcc
      241 gacgccgagc tccatcagcg ccagcgtgtg gcgcacgtcg caggcgcgcg agatcgtgcg
      301 cagcctcggg aagtgatgac gcaccgtgcg cgcgatgcgc agcgaggtct cgccgtcatc
      361 gaccgcgatc accagcacat gcgcgtcgcc ggccgaggcc gcgcgcagca gctcgatgcg
      421 cgaggcatcg ccgaagtgca cggtctggcc gagggcgcgg ccgagttcga tgcgcttgag
      481 gtcggtctcc agcaccgtgc aggggatgcc gtgcacgcgc aggtagcggg cgacggtctg
      541 gccgaagggg ccgtagccgg cgatcagcac cggcgccgcg gcgatcggct cggcctgcgc
      601 cggggagcgc cggcgccggc gctcggcgag ccgctcggcg aggctgtcgg tgaggcgcac
      661 cagcatcggg ctcgccgcca tcgacagcgt cacggcgagg ttcagctcgt cctgctggcg
      721 cgtggtcagc agcgccagat cgcgcgcgac cgcaaacagc acgaacgcga actcgccgcc
      781 ctgcgcgagc agcaccgcga ccttgcaggc cgggcgcggc gcggtgccga acagccgggc
      841 gatcaggaac agcagcagcg ccttcaccgc gatcagcgcc gcggtcagcg ccagcagcac
      901 gccgggttcc ttggtcagca ggccgaggtt cgccgacagc ccgacggcga cgaagaacag
      961 cccgagcagc aggcccttga acggctcgat gttggcttcg agctcgtggc ggaactcgga
     1021 atccgacagc agcatgccga ccaggaaggc cccgagcgac atgctcaggc cgatgtgcgt
     1081 gaagccgagc gcggttgccg cgaccacgag cagcgccgca gccgtggcga tctcgtgcga
     1141 atgcgcggcg gcgaccagcc ggaacagccg cggcagcagg tagcgcccgg cgagcacgac
     1201 gccggcgatc gccagccccg cgaccagcgc cttctgcagg tcctcgccgg gttcgaagcg
     1261 ctcgctcggc gcgaacagcg gcaggatcgc cagcatcggg atcaccgcca cgtcctgcat
     1321 cagcagcact gcgaaggcgt cgcggccgtg gcgcgcggtc agctcgccgc gctcgccgag
     1381 cagctgcagc acgaaggcgg tcgaggacag cgacagcccg aagccgatca cgaaggccgt
     1441 gcccggatgc cagccgagcc actgcatcac cgccatcacc gcgccgccgg tcagcagcac
     1501 ctgcaggccg ccgagcccga acaccggccg gcgcagcgcc cacagccgcg agggctgcag
     1561 ttcgaggccg acgatgaaca gcagcagcac gacgccgagt tcgccgacgt gcaggatttc
     1621 ctcgtgctcg cggatcagcg ccagccccca cggcccgatc agcacgccgg cgagcagata
     1681 gccgatcacg gccccgagcc cgagccgctt cgcgagcggc acggccagca ccgcggcggc
     1741 gaggatgacg acgattttcg cgagatcgga cacgatggcg gttgcggcgg caggacggcg
     1801 ggaatgcgtg cccgacagtg tgcctgagcc agatgacggc gcgccgcgtc agtccgccgg
     1861 gtcgatcagg ccggtcagcc agtcctccag cgccgcggcc ttgtcggcaa ccggcgcgct
     1921 gccggcgagg atggtcttcg gcgtttcgaa ggcgctcgcg aaatcctcgc cggtcagcca
     1981 cgcgcaggcc gcgtagacga agccggtgtc ggcatcggca aagccgtgca gcgcggcgtg
     2041 gcgcagcacc gggcgcagga aggcgtgcag ttcggcatcg gactgctcgc tcgcgtcctc
     2101 gaactcggcg cgcagccgcg cggcgacctc gggcagcagc gcctcgacgg cggcgaaatc
     2161 gagcatggcg gcatctccgc ggcttcggga acggaggcgg cacggtagcc gcgcgcccgg
     2221 ctgcggtcca ccgccggcca tgccagccgg aacttccgcc cgggcgtgcg gctctgtcgg
     2281 gatgtgccgg gcccgtcccg gacagctcac gccgctgtga cacggggccc cgagcggcga
     2341 caacgaaccc gaaccccgga gcttcgatca tgaaccgacc tgccacgcgt tccgtccatc
     2401 tcgccggcag tgccgcgctc gcggtgctgc tgattgccgg cggtctggcc gcgccgcgcg
     2461 cgcacgccga tccgcgtccg cacacggtga tcaccgagga agtccgtcag gaagtcacgg
     2521 tcagccgcca ctcggtcggc ccggccacca ccccgccacc agcgccgacc gtcaccgaaa
     2581 tgaccccccc gccgccgccc ggcgccgtgg tcgccgccga cgtgccgccg ccgccgccgc
     2641 gcgtcgagca gccggtcggc cagccggccc cggaataccg ctgggtgccg ggccactgga
     2701 gctgggagaa cggcgcctgg gcatggacgc cgggcacctg ggagcagccg ccgcagcaga
     2761 gtgccgaatg ggtgccgggc cgctgggagc agcgtccgga cggctggctg tggattccgg
     2821 gctactggtc gtaagcccgg cgccggccgg cgccggcgcg tgaggttttc cgcgccgatc
     2881 ttcaccggga agccggatac gcgcttcccc gcactccacc ggaaacaccg cgggccctgt
     2941 cgccggatcg gcgcagggcc cgcggccgtt tgcggcccgc tcagccggcg gcttccggca
     3001 ccgcccgcac ccaggcctgc atgttgtcga gcttcgtgtc gccgaacggg gtcgccagcg
     3061 ccacgtcgag cgcatcgcgg ccgtagccga tgatcacgcg cccgccgcgc ggcccggcct
     3121 gcgtggcgtc gaaggtgtac cagcgcccgt cgacatacgc ctcgaaccag gcgtgcaggt
     3181 ccatcggctc cagttcgtgc aggtagccgg cgacgtagcg ggccgggatg ccgagcgcgc
     3241 gcgtcagcgc gatgccgacg tgcgcgaagt cgcggcacac gcccttgcgc atgcggatcg
     3301 tgtcgcgcgc cgaggtcgat tcgttgctga cgccgtagcg gtattcgaag ctgcggtgga
     3361 tccaggcgcg gatcgcctcg acctggtcgt agccgggcgt ggcgccggcc gtcacgtcgc
     3421 gcgcctgatc ggcgagcgcg tcggattcgg cgtagcggct ggcgagcagg aagccgatga
     3481 cctcgaccgg cagctgatcg accggcgtgc gcggcgcccc gggcgcgagc gcgatcgtgt
     3541 catcggtgtc gacgcgcacc tgcgtcgaga tgtgcagccg cccgggccgg gccacgatgc
     3601 gctggcagag attgccccag gcatcggcgt actcgctggc cggcacgcgc ggctgcagcg
     3661 cgtagcgttc cgcttccacc cactgacctt cgccgctgcg cggacgcagc atcagcagca
     3721 tcggcgtcgg ttcgaggatt tcaaattcga gctcacagcc ggcatcgagg cgcatggcgg
     3781 attctctctc cgtatggcgc gccggcaccc agcgcgccgg ccgcgtgcgc aggcagacgg
     3841 cgcgcggacg cggaagttcg ccccgcgccg gcgcctcagc ggccgtaatc cggaaagaac
     3901 agctgctcgc cgttgatccg gtagccggcg atggcctgct ggccttccgg cgagatcacc
     3961 cagtcgatga aggcctgggc gtcggcctgc ctgacgtgcg ggaacttcgc cgggctgacc
     4021 agcaggatgc cgtactggtt gaacagcctc ttgtcgccct cgacgacgat cttcagcggc
     4081 ccgcggttcc tgaacgacag ccaggtgccg cgatcggtca gcgcgtaggc cggcaggccg
     4141 gcagcggtgt tcagcgtcgg ccccatgccc gagccggtct cgcgatacca gctaccggaa
     4201 gcggccttca cgtcgatgcc ggccgccttc cacagcgcct gttcggcctt gtgcgtgccg
     4261 gaatcgtcgc cgcgcgaagc gaacggagcc tgctttccgg cgatggccct gaaggccgcg
     4321 accacgtcgt gtccgccctc gatggcggcc gggtcggtgc cgggaccgac gacgatgaag
     4381 tcgttgtaca tgaccgggaa gcgctgcacg ccgtagccct cggcgacgaa cttctcctcg
     4441 gagggctggt ggtgcacgaa cagcacgtcg gcatcgccct gctgggcgat cttgatcgcc
     4501 tgaccggtgc cgaccgcgac cacgcgcacc tcgatgccgg tctttccggt gaacttcggc
     4561 agcaggtaac caaagagccc ggactgctcg gtcgaggtgg tcgaggccac ggtgatgaag
     4621 cgctcggccg cctgcaccgg cagcgacagc accgacagca gcagcgcaca gaggccggcg
     4681 cgcaggcccg gcagcgaagg acggaaggac atcgcgacga ctcctcgggg gcttctgtct
     4741 cgggtttttt tcgtcatggg gcatgcacgg cgccgcagcc gcgcggcccc tcaggccggc
     4801 acggcgagca gcacgctcga agccttgatc agcgcgcagg cgcgccgccc cggtgccagc
     4861 ccgagttcgt ggacgctggt gtgcgtcagc gtcgcggtga cgcgctggcc ggcgtcgagg
     4921 cgcatggaaa tctcgtcgtt gacggcgccg gtgcgcagcg catcgacgac gccccacagc
     4981 gcgttgcgcg ccgagacgaa gggcatgcgc tcggtcgtga agatcacgct cgacgcctgc
     5041 accagcgcat agacttcccg gcccggacgc agcgtcagcg cgtcgatgct ctcgtcggtg
     5101 acgatcgcca ccatcgaggc cccgccgccg atgtcgatca gcacctccgc gttgaccgcg
     5161 ccgcgctcga ccgtgacgac atgaccgtga atctggttgc cggcgctgac cttcagcggc
     5221 gggcgtggca gggtggacga cgcgctggac acggcgacgg acctcggtga cggacggcag
     5281 ggatggcccg gtcggctggc cggacgcggc agactttata tgaatcaaaa tatatagcga
     5341 agtacagtgg cgactctcaa gcccatgagc ccgatcgatc ctccgtcccc ggccgacatc
     5401 gccacgcagc tcgccttctt cgcgcgcgac gggcagacgc tgctgaagcc cgagcgcgtc
     5461 gcactgctcg aacacatcca gcacagcggc tcgatctcgg ccgccgcgcg cgcgatcccg
     5521 atgagctaca aggcggcctg ggatgcgatc gacgcgatga acaacctcgc gccgcagacg
     5581 ctggtcgagc gcgtcgccgg cggccagcgc ggcggcggtt cgcggctgac cgagtacggg
     5641 gcgcgcatcg tcgcgcgcta ccgcgcgatc gagcgcatgc agcgccagct gattgatctg
     5701 attgccatgg acgatccgca gatgctcggc gacctgcccg gtctcggcct gctgttcggg
     5761 ctgcgcacga cggcgcgcaa cctgttttcc gggcgggtgg cgcgggtcgc ggccggtgcg
     5821 gtgtcgagcg aggtggtcat cgacatcggc gcccaggacc ggctggtggc catcgtcggg
     5881 cgccaggatg tcgaggccct gcgcctggtg ccgggggcgg cggtgctggc gctggtgcag
     5941 gccacgcagg tgatgctggc gctggcggag gccgggctgc gcaccagcgc ccgcaaccgc
     6001 ctcgacggca ccgtgctctg catcggcccg ggcgccgtca atgccgaggt gcagatcgcg
     6061 ctcgccggcg gcaagcggct gacggcgatg atgacgatgg aaagcctgca ggcgctcggc
     6121 ctcgaaccgg gtcagccctg cacggcctgg atcaaggctt cgagcatcgt gctggccgcc
     6181 atcggctgaa gccccggcac ccgccccgcc gcggtttcgc ctacctcttt cgtatcaatc
     6241 agttgtatgt gtcgcacccg gtgcagcgcc ttgtctgcgc cggagcggcg tcacagcctg
     6301 tgccgctgca gggacgattt ccattggctt tttcatggga atgaattttc cgcaatgtca
     6361 caaactcctg ttttcccaca ggaagttttt ttatgaatgt cactttatag aatggcatcc
     6421 atattgcgat ggctctcctg cccaaactgg cacgaccgca gcagacggcc gtgacgacca
     6481 cccatcaaag aagatgcagg agagcctcat gatttatgca aagcctggcc agcccggttc
     6541 gaaggtcacc ttcaagtcgc gctacgccaa cttcatcggc ggcgagtggg tgccgccggt
     6601 cgaaggccgc tacttcgaga acgtctcgcc ggtcgacggc aaggtgttct gcgagattcc
     6661 gcgctcctcg ccggccgaca tcgagctggc gctcgatgcc gcacaccgcg ccaaggatgc
     6721 gtggggcaag gcctcgccga ccgagcgttc gaacaccctg ctgaagatcg ccgaccgcat
     6781 ggaagcgaac caggagatgc tcgcggtcgc cgagacctgg gacaacggca agccgatccg
     6841 tgaatcgctc gccgccgaca ccgcgctcgc gatcgatcac ttccgctact tcgccggctg
     6901 catccgcgcg caggaaggtt cgatcggcga gatcgaccac gacacggttg cctatcactt
     6961 ccacgagccg ctcggcgtcg tcggccagat catcccgtgg aacttcccgc tgctgatggc
     7021 ggcgtggaag atcgccccgg cgctcggtgc cggcaactgc atcgtgctga agcctgccga
     7081 gcagaccccg gtctcgatcc tcgtgctcgc cgagctgatc caggacatcc tgccccccgg
     7141 cacgctgaac atcgtcaacg gcttcggccg cgaagtcggc caggcgctcg ccaccagcaa
     7201 gcgcatcgcc aagatcgcct tcaccggctc gaccgccacc ggccgtctgg tcatgcagta
     7261 cgcgaccgag aacatcatcc cggtgacgct cgaactcggc ggcaagtcgc cgaacatctt
     7321 cttcgacgac gtgatggcga aggacgactc cttccgcgag aaggcgctcg aaggcttcgc
     7381 gatgttcgcg ctgaaccagg gcgaggtctg cacctgcccg tcgcgcgcgc tggtgcagga
     7441 atcgatcgcc gacgagttcc tcgcgctcgc cgtcgagcgc gtcagccgca tcaagcaggg
     7501 tgatccgctc gacaccgaca ccatggtcgg cgcgcaggcc tcggccgatc aggtcgagaa
     7561 gatcatgtcg tacatcgaca tcggccgtca ggaaggcgcg cagtgcctga tcggcggcga
     7621 gcgcaaccag ctcggcgaca gcctcggccg cgggtactac atcaagccga cggtgttcaa
     7681 gggcaacaac aagatgcgca tcttccagga ggagatcttc ggccccgtgc tgtcggtcac
     7741 gaccttcaag gacgaagccg aagcgctgga aatcgccaac gacaccatct tcggcctcgg
     7801 ctccggcgtc tggacccgcg acggcgtgcg cgcctaccgc atgggccgcg gcatccaggc
     7861 cggccgcgtc tggaccaact gctaccacca ctacccggcg catgccgcgt tcggtggcta
     7921 caagcagtcc ggcatcggtc gcgagacgca caagatgatg ctcgaccact accagcagac
     7981 caagaacctg ctggtctgct acagccagaa
//
LOCUS       DAOQIE010000178         7997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4649_length_7997_cov_6.669101, whole genome shotgun sequence.
ACCESSION   DAOQIE010000178 DAOQIE010000000
VERSION     DAOQIE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4649_length_7997_cov_6.669101"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PK144_11570"
     CDS             <1..1401
                     /locus_tag="PK144_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNF66413.1"
                     /translation="LAACTVGPDYARPAAPVPGEYREADGWQRAQPADARLRGDWWTI
                     YGDPQLDALVREAEVSNQNLALAEASLRQAQALAREAGAALYPVAGVGAGVTRARSSA
                     AGATGTAGGRTTSQYSLPLELSWEIDLWGRLRRGAEAGQATAEASAADLEAARLSVRA
                     ELVTNWLALRTLDARRRLLADTVEAYARALAMTRNRHAAGVASGAEVAQAETQLESTR
                     AQALDLDSSRATLEHAIAVLAGRPPATLRIAVREGPLPPVPALPAGLPSTLLERRPDI
                     AAAERRVAAANAGIGVAEAAYYPSLNFSASAGFEAASLAKWISAPARVWALGPQLALT
                     LFDGGARAAQVEAAGASWQGTVASYRQTVLTGFQEVEDALATLRILAAEAAVQDRAVT
                     AARRSLELTMNQYRAGILAYLGVVVVQAQALAAESSAIDLQGRRLAASVALIRALGGG
                     WQAGDPSPALTRADTR"
     gene            1568..1831
                     /locus_tag="PK144_11575"
     CDS             1568..1831
                     /locus_tag="PK144_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66414.1"
                     /translation="MNATAASSPKIESDRTARRLAADLPPGYTLRGFDLGHGTEWWVC
                     DERGQIVDGNRLGAGHGRQRFQVALLLALCMAGLVVALGLYAG"
     gene            complement(1906..2100)
                     /locus_tag="PK144_11580"
     CDS             complement(1906..2100)
                     /locus_tag="PK144_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNF66415.1"
                     /translation="MTKNVGQIDKILRLLVGLVLIALTLSGQIGVWGWIGVLPIATAL
                     MNWCPAYTLLGVRTCPVEPK"
     gene            2399..2713
                     /locus_tag="PK144_11585"
     CDS             2399..2713
                     /locus_tag="PK144_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF66416.1"
                     /translation="MDRSANAAAQLLKALASPVRLKILCQLVDGEKAVGQLADLLQAR
                     DALVSQHLALLRKDGLVRTRREAQTIWYSIASPAAERIIRTLYELYCAGDGAGPAPGP
                     KP"
     gene            2740..3285
                     /locus_tag="PK144_11590"
     CDS             2740..3285
                     /locus_tag="PK144_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HNF66417.1"
                     /translation="MSLTRLDARSLQVRLAEGRADLYDIREPAEYAREHIDGARLLPA
                     GRLGRELPDRTPGRLAVFMCQSGMRTTENATRILGCISGEVAMLDGGLEAWKRAGLPV
                     IEDRSAPLPLMRQVQIVAGSLVLLGVVLGFLLGPAWFGLAGFVGAGLVFAGLTGFCGM
                     ARLLAQLPWNRIDPPSAPRAV"
     gene            complement(3296..3472)
                     /locus_tag="PK144_11595"
     CDS             complement(3296..3472)
                     /locus_tag="PK144_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HNF66418.1"
                     /translation="MSDHPALPPRPPRPHPLECCGRGCRPCVFDYWRRAVADWEAECR
                     RRGIDPPPAAHDAG"
     regulatory      3587..3698
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            3803..4405
                     /locus_tag="PK144_11600"
     CDS             3803..4405
                     /locus_tag="PK144_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNF66419.1"
                     /translation="MEAFLVSTFVVAIAEIGDKTQLLALILAARFRRPWPIVLGILVA
                     TIANHFAAGALGAAVASLFGPELLRRALGISFLAVAVWALIPDRMDEDEPVPASAHGA
                     FIATTVAFFLAEIGDKTQIATVALAARFDALAWVVAGTTIGMMLANVPAVLLGHRLGR
                     AAFALGWVRYVAAATFALLGLAALFDVNLDPSGWFGHTPG"
     gene            4608..5228
                     /locus_tag="PK144_11605"
     CDS             4608..5228
                     /locus_tag="PK144_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNF66420.1"
                     /translation="MRLRYSPTSPFVRKISVAALELGLAERITRVPTNPWDPASDIAH
                     DNPLGRIPALVLDDGSGTVLYDSPVIVEYLDALAGGGRLLPAAGPARWAALRRAALAD
                     GIIDAGIAVLVERVRRPEAYRWPEWIEFQTLAITRGLAGFDAEITQLSTDAPGIAEIG
                     LMCALDWLDFRGIGSGWRDAHPALVAWHGQLALRPSFAGTLPKDPA"
     gene            5296..6468
                     /locus_tag="PK144_11610"
     CDS             5296..6468
                     /locus_tag="PK144_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF66421.1"
                     /translation="MQADPILFAVFLIFTGAAVLAALALFARQSLLIAYIALGGLCGP
                     AGFNLVAEPRVIEQIGHVGIIFLLYLLGLNLHPQKLLTMLREATRVTAISSAAFAAVA
                     AIIVWGFGYTFAEGIVIGAVMMFSSTIIGLKLLPTTALHQRHMGEVMISVLLLQDILA
                     IFILLLLQGAGQDGGTDWLALGQRLLALPLLFGLALLLERKLLMPLIARFDVIQEYVF
                     LTAVGWCLGMAQLAHALGLSYEIGAFVAGVALATSIIATFIAESLKPLRDFFVIMFFF
                     SVGASFELGALGSVLLPALVLAAAMLWVKPRVFTRLLLAGGEKPRLAYEIGVRLGQVS
                     EFSLLIAVLATQAGVIGTRASSLIQAATLLTFIASSYWVVRRFPTPMAVNDRLRRD"
     gene            complement(6498..6851)
                     /locus_tag="PK144_11615"
     CDS             complement(6498..6851)
                     /locus_tag="PK144_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNF66422.1"
                     /translation="MSAEHPPILDPTVLGELRELLEDAYGDLLQNFLDDLAEQLPAME
                     EALMRGAAMTLGQIAHKLKSASGSVGALRLSALAFELEQLGKSGDVSRALPVLAQLEA
                     VAHDTQRAISAELAT"
     gene            7016..7498
                     /locus_tag="PK144_11620"
     CDS             7016..7498
                     /locus_tag="PK144_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNF66423.1"
                     /translation="MALEIEHKFLVTGDGWRQAVERSVRFSQGYLVSDDIRSVRVRLE
                     GDEARLNIKRSGIGTSRAEYEWPIPPAEAAEILATLCGAKVEKTRHFVPHAGHLWEID
                     EFAGDNAGLVVAEVELAFEGEPFARPDWVGADVSHELRYYNSQLAVRPYRDWTDAERG
                     "
     gene            7495..>7997
                     /locus_tag="PK144_11625"
     CDS             7495..>7997
                     /locus_tag="PK144_11625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNF66424.1"
                     /translation="MNGLLAPVAHVPRVRVDLAGQRLWLLAADGRIEAGFAVSTAANG
                     PGERMHSGCTPRGRHRIRAKIGAAAPVGTVFRGRRPTGETWSPAYAATQPARDWILSR
                     ILWLGGLEPGRNRYGDCDSQRRYIYIHGTPDTEPMGVPRSHGCIRMRNADVIELFDRV
                     HAGDEVW"
BASE COUNT         1034 a         2868 c         2854 g         1241 t
ORIGIN      
        1 ctcgccgcct gcacggtcgg ccccgactac gcgcgcccgg ctgcgccggt gccgggcgag
       61 tacagggagg ccgacggctg gcagcgcgcg cagccggccg atgcgcggct gcgcggcgac
      121 tggtggacga tctacggcga cccgcagctc gatgcgctgg tgcgcgaggc cgaagtctcg
      181 aaccagaacc tcgcgctcgc cgaggccagc ctgcgtcagg cgcaggcgct ggcgcgcgag
      241 gccggcgccg cgctgtatcc ggtggccggg gtcggtgccg gcgtcacgcg cgcgcgcagc
      301 agcgccgcgg gtgcgaccgg cacggccggc gggcgcacga ccagccagta ttcgctgccg
      361 ctggagctgt cctgggagat cgatctgtgg gggcggctgc gccgtggtgc cgaggccggt
      421 caggcgacgg ccgaggcgag cgcggccgat ctcgaggccg cgcgcctgag cgtgcgcgcc
      481 gagctggtca cgaactggct ggcgctgcgc acgctcgatg cccggcgccg gctgctcgcc
      541 gataccgtcg aggcctacgc gcgcgcactc gccatgacgc gcaaccgcca cgccgccggc
      601 gtcgcctccg gcgccgaggt cgcgcaggcc gaaacccagc ttgaatcgac gcgcgcgcag
      661 gcgctcgatc tcgacagcag ccgcgcgacg ctcgaacacg cgatcgccgt gctcgccggc
      721 cgcccgccgg cgacgctgcg catcgccgtg cgcgagggtc cgctgccgcc ggtgcccgca
      781 ctgccggccg gcctgccctc gacgctgctc gaacgccggc ccgacatcgc cgcggccgaa
      841 cggcgggtcg cggcggccaa cgccggcatc ggtgtggccg aggccgcgta ctacccgagc
      901 ctgaatttct cggcctcggc cggcttcgag gcggcgagcc tcgcgaagtg gatctcggcg
      961 ccggcgcggg tctgggcgct cggtccgcag ctggcgctga cgctgttcga cggcggggcc
     1021 cgcgccgcgc aggtcgaggc ggccggtgcg agctggcagg gcacggtcgc gagctatcgc
     1081 cagacggtgc tgaccggctt ccaggaggtc gaggacgcgc tggcgacgct gcgcatcctc
     1141 gcagccgagg ccgcggtgca ggatcgggcc gtcaccgccg cgcgccgctc gctggaactg
     1201 acgatgaacc agtaccgcgc cggcatcctc gcctacctcg gcgtcgtcgt cgtgcaggcg
     1261 caggcgctgg ccgccgagag cagcgcgatc gacctgcagg gccggcggct cgcggccagc
     1321 gtcgcgctga tccgcgcgct cggcggtggc tggcaggcgg gtgatccctc gccggcgctg
     1381 acgcgggccg acacccgctg acgcgcagcg ccagcttgtg acgcctgctg tcaggagcgt
     1441 cgtgccgggt tacgacttgg tcatgtttct cttcccggcc ttggttctgc cgcgacgcac
     1501 aagcagaatc gttgcgccgc atccaagcgg gtgcggcagc ccctcaccgc agagttgcac
     1561 ggatgccatg aacgccactg ccgcaagctc cccgaagatc gaatccgacc ggaccgcccg
     1621 ccgcctcgct gccgatctgc cgcccggcta cacgctgcgc ggtttcgacc tcggtcatgg
     1681 caccgaatgg tgggtctgcg acgagcgggg tcagatcgtc gatggcaacc ggctcggcgc
     1741 cggccacggt cgccagcgtt ttcaggtcgc gctgctgctg gcgctgtgca tggccggtct
     1801 ggtggtcgcg ctcgggctgt atgccggctg aagcgcgctg atccgcccgg atgaaaaaag
     1861 ccccgctgat gcggggcttt tcgtttgtgc cggcgggcgc gcgacttact tcggctcgac
     1921 cgggcaggtg cgcacgccga gcagcgtgta ggccgggcac cagttcatca gcgcggtggc
     1981 gatcggcagc acgccgatcc agccccagac gccgatctgg ccgctcagtg tcagggcgat
     2041 cagcacgagg ccgacgagca ggcgcaggat cttgtcgatc tgtccgacgt tcttggtcat
     2101 gacgaatgtc ctccgggatg gtggatgccg ggtaggcgtg acggctctgt gttttccgct
     2161 ctttccgaac cctttccccc agggggaggg gctcgggaag acggtgtgtt ccgccgggac
     2221 gggtatgaaa gacctgcccg cgaccgatgc ccggcatcgg cgcgcgagac gttccgctag
     2281 gctggcagac ccgtctacac cgcggcggcg ggtgacattc ccttgccatg catcgggttt
     2341 ccgctgtttg acagcataat catgtattcg tatattcgca aatatgaaca ttgacgagat
     2401 ggaccgctcc gcgaacgccg cggcgcagtt gctgaaggcc ctcgccagtc cggtgcgcct
     2461 gaagatcctc tgccagctcg tcgatggcga gaaagccgtc ggccagctcg ccgatctgct
     2521 gcaggcccgc gacgcgctgg tctcgcagca tctggcgctg ctgcgcaagg acgggctggt
     2581 gcgcacgcgg cgcgaggcac agacgatctg gtactcgatc gcgagcccgg cggccgaacg
     2641 catcatccgc acgctctacg agctgtactg cgccggcgac ggcgcgggac cggctcccgg
     2701 acccaagccg taaccgactg actgacggag acctgcccca tgagcctgac ccgcctcgat
     2761 gcccgcagcc tgcaggtgcg cctcgccgag ggccgtgccg acctgtacga catccgcgag
     2821 ccggccgagt acgcccgcga gcacatcgac ggcgcgcggc tgctgccggc cggccggctc
     2881 ggccgcgagc tgcccgatcg cacgccgggg cggctcgcgg tgttcatgtg ccagagcggc
     2941 atgcgcacca ccgagaacgc cacgcgcatc ctcggctgca tcagcgggga ggtggcgatg
     3001 ctcgacggcg ggctggaggc ctggaagcgc gccggcctgc cggtgatcga ggaccgctcg
     3061 gcgccgctgc cgctgatgcg tcaggtgcag atcgtcgccg gcagtctggt gctgctcggc
     3121 gtcgtgctcg gcttcctgct cggcccggcc tggttcgggc tggccggctt cgtcggtgcc
     3181 ggcctcgtgt tcgccggcct caccggcttc tgcggcatgg cgcggctgct cgcgcagctg
     3241 ccgtggaacc gcatcgatcc gccgtccgcg ccgcgcgccg tctgaagccg gggggtcatc
     3301 cggcgtcgtg cgccgcgggc ggcggatcga tgccgcgtcg ccggcactcg gcctcccagt
     3361 cggcgacggc gcgtcgccag tagtcgaaca cgcagggccg gcagccgcgt ccgcagcatt
     3421 cgagcggatg cgggcgcggc ggacgcggtg gcagggcagg gtggtcggac atcacgggct
     3481 tccacgcacg gggagagggc aattatcgcc gctgcggctg catgcgatgc gggcgtccgg
     3541 cgcaccgccg gctgattgac gcgctgcagc agacacggct agcctgtgca ctcccatcgg
     3601 ggagtagtcg ccctgcctgc acgcgagggg cgcgcgtcaa cagacttgcc gctgcacggc
     3661 ggcatggcgc gcgcagtccg gaccggagct tccggtccgg ggctggcgag acctttggca
     3721 tcggtgccgc aagtccgggt cgggcgggcg gcgccgggcc atcggcacgc gtcttcgccc
     3781 gaccccgcgt atccgcgctc ccatggaagc cttcctcgtc tcgaccttcg tggtcgccat
     3841 cgccgagatc ggcgacaaga cccagctgct cgcgctgatc ctcgccgccc gcttccggcg
     3901 gccgtggccg atcgtgctcg gcatcctcgt cgccaccatc gccaatcact tcgccgccgg
     3961 cgcgctcggg gcggcggtcg cgagcctgtt cgggccggag ctgctgcgcc gggcactggg
     4021 gatttccttc ctcgcggtcg cggtctgggc gctgatcccg gaccgcatgg acgaggacga
     4081 gccggtgccg gccagcgccc acggcgcctt catcgccacg acggtggcct tcttcctggc
     4141 cgagatcggc gacaagaccc agatcgcgac cgtcgcgctc gcggcgcgct tcgatgcgct
     4201 cgcgtgggtg gtcgccggca cgacgatcgg catgatgctc gcgaacgtgc cggccgtgct
     4261 gctcggccac cggctcgggc gcgcggcctt cgcgctcggc tgggtgcgct acgtcgccgc
     4321 cgcgaccttc gcgctgctcg ggctggcggc gctgttcgac gtgaacctcg atccgtccgg
     4381 ctggttcggg cacacgccgg gctgacgggc atcgccggcg ggaacggcgc ggcctcccgc
     4441 cggcatcgca agcgcgaccc gcatcgtccg ccgcacgcac cggccgcctg ccggtgcgtg
     4501 catgccagcg atgcagcagg cgcgggctgc gccgtccaga cttcgcggcg cccggcacgg
     4561 ccccgacgcg gaccgtgcct tccgtccgcc gcttccggag cctgcccatg cgcctgcgct
     4621 attcgcccac gtcgcccttc gtccgcaaga tctcggtcgc cgccctcgaa ctggggctcg
     4681 ccgagcgcat cacgcgcgtg ccgaccaacc cctgggatcc ggcgagcgac atcgctcacg
     4741 acaatccgct cgggcgcatc ccggcgctcg tgctcgatga cggcagcggc acggtgctct
     4801 acgacagtcc ggtcatcgtc gaatacctcg atgcgctcgc cgggggcggg cggctgctgc
     4861 cggcggccgg gccggcgcgc tgggcggcgc tgcgccgggc cgcgctcgcc gacggcatca
     4921 tcgatgccgg catcgcggtg ctggtcgagc gcgtgcgccg gcccgaggcc taccgctggc
     4981 cggagtggat cgagttccag acgctggcga tcacgcgcgg cctcgcgggc ttcgatgccg
     5041 agatcacgca gctgtccacc gatgcgcccg gcatcgccga gatcggcctg atgtgcgcgc
     5101 tcgactggct ggacttccgc ggcatcggca gcggctggcg cgatgcgcat ccggcgctcg
     5161 tcgcctggca cgggcagctg gccttgcggc cgtccttcgc cggcacgctg ccgaaggacc
     5221 cggcctgacg gtgcacggct ggcggaatcg gcgaggcccg acgatgcttg cggctcgccg
     5281 cgaggaccgc ccgccgtgca agccgatccg atcctgttcg ccgtattcct gatcttcacc
     5341 ggtgccgccg tgctggcggc gctggcgctg ttcgcgcgcc agtcgctgct gatcgcctac
     5401 atcgcgctcg gcggcctgtg cggcccggcc gggttcaatc tggtcgccga gccgcgcgtg
     5461 atcgagcaga tcggtcacgt cggcatcatc ttcctgctct atctgctcgg tctgaacctg
     5521 cacccgcaga agctgctgac gatgctgcgc gaggcgacgc gcgtcacggc gatcagctcg
     5581 gcggcgttcg cggccgtcgc ggcgatcatc gtctggggct tcggctacac cttcgccgaa
     5641 ggcatcgtca tcggcgcggt gatgatgttc tccagcacca tcatcggcct gaagctgctg
     5701 ccgaccacgg cgctgcacca gcggcacatg ggcgaggtga tgatctcggt gctgctgctg
     5761 caggacatcc tcgcgatctt catcctgctg ctgctgcagg gcgccggcca ggatggcggc
     5821 accgactggc tcgcgctcgg gcagcggctg ctggcgctgc cgctgctgtt cgggctggcg
     5881 ctgctgctgg agcgcaagct gctgatgccg ctgatcgcgc gcttcgacgt gatccaggag
     5941 tatgtctttc tgaccgcggt cggctggtgc ctcggcatgg cgcagcttgc gcacgcgctc
     6001 gggctgtcct acgagatcgg cgccttcgtc gccggcgtcg cgctcgcgac cagcatcatc
     6061 gcgaccttca tcgccgagag cctgaagccg ctgcgcgact tcttcgtgat catgttcttc
     6121 ttctcggtcg gcgcgagctt cgagctcggt gcgctcggct cggtgctgct gccggcgctg
     6181 gtgctcgcgg cggcgatgct gtgggtcaag ccgcgcgtgt tcacgcgcct gctgctcgcc
     6241 ggcggtgaaa aaccacggct ggcctacgag atcggcgtgc gcctcggtca ggtcagcgag
     6301 ttctcgctgc tgatcgcggt gctggcaacg caggccggcg tcatcggcac ccgggcctcg
     6361 tcgctgatcc aggcggcgac gctgctgacc ttcatcgcca gctcgtactg ggtggtgcgc
     6421 cgtttcccga cgccgatggc ggtcaacgac cggctgcgcc gcgactgagg cgcggcgcga
     6481 agacgcaggt cggggggtca ggtcgccagt tcggcgctga tcgccctctg cgtgtcgtgc
     6541 gcgacggctt cgagctgcgc gagcaccggc agcgcgcgcg agacatcccc ggacttgccg
     6601 agctgttcga gctcgaaggc cagggccgac agccgcagcg cgccgacgct gccgctcgcg
     6661 gacttgagct tgtgtgcgat ctggccgagc gtcatggcgg cgccacgcat cagggcctct
     6721 tccatcgccg gcaactgttc ggcgagatcg tcgaggaagt tctgcaacag gtcaccgtag
     6781 gcatcttcga gcagctcacg cagctcgccg agcacggtcg gatcgaggat cggcgggtgt
     6841 tcagcactca tcggcggtat ccagggggca gggcgggcga agatataccc tcgcccgggc
     6901 atccattaac atccgtccac tcacggccgg cgtgcttgtt gcaaaccctc acagtgtctg
     6961 caaccggttc gcgcttgcat ccgaatcttc gcatccgggg acgggtttcg caccgatggc
     7021 gctcgagatc gaacacaaat ttctggtcac cggcgacggc tggcgacagg ccgtcgagcg
     7081 cagcgtgcgc ttcagtcagg gctacctggt cagcgacgac atccgctcgg tgcgcgtgcg
     7141 cctcgaaggc gacgaggccc ggctgaacat caagcgctcc ggcatcggca ccagccgcgc
     7201 cgagtacgaa tggccgatcc cgccggccga ggccgccgaa atcctcgcga cgctctgcgg
     7261 cgcgaaggtc gagaagaccc gccacttcgt gccgcacgcc gggcatctgt gggagatcga
     7321 cgagttcgcc ggcgacaacg ccgggctggt ggtcgccgag gtcgagcttg cctttgaagg
     7381 tgagccgttc gcgcgcccgg actgggtcgg ggccgatgtc tcgcacgagc tgcgctacta
     7441 caactcccag ctcgccgtgc gcccgtaccg cgactggacc gacgccgagc gcggatgaac
     7501 gggctgctgg caccggtggc gcacgtgccg cgcgtgcgcg tcgatctggc cggacagcgg
     7561 ctgtggctgc tcgcggccga tggtcgcatc gaggccggct tcgcggtctc gaccgctgcc
     7621 aacggccccg gcgagcgcat gcacagcggc tgcacgccgc gcggccgcca ccgcatccgc
     7681 gcgaagatcg gcgccgctgc gcccgtcggc acggtgttcc gcggccgccg cccgaccggc
     7741 gaaacctgga gtcccgcgta tgccgccacc cagccggccc gcgactggat cctgagccgc
     7801 atcctgtggc tcggcggcct ggagccgggc cgcaaccgct acggcgactg cgacagccag
     7861 cgccgctaca tctacatcca cggcacgccc gacaccgaac cgatgggcgt gccgcgctcg
     7921 cacggctgca tccgcatgcg caacgccgat gtcatcgagc tgttcgaccg ggtgcacgcc
     7981 ggcgacgagg tctggat
//
LOCUS       DAOQIE010000179         7938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4713_length_7938_cov_7.617024, whole genome shotgun sequence.
ACCESSION   DAOQIE010000179 DAOQIE010000000
VERSION     DAOQIE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4713_length_7938_cov_7.617024"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..407)
                     /locus_tag="PK144_11630"
     CDS             complement(78..407)
                     /locus_tag="PK144_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66425.1"
                     /translation="MNPAALEAMIARGQDGALLRTTLGKLYLDTGDAARAHAHLERAV
                     GLDAQYSAAWKLLGHARAALGDPAGALAAWTQGIAVAEARGDLQAAKEMAVFHRRLLR
                     SLPPDAP"
     gene            complement(404..976)
                     /locus_tag="PK144_11635"
     CDS             complement(404..976)
                     /locus_tag="PK144_11635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF66426.1"
                     /translation="MPVTDRDRWDARYRDGIPQQRPCATLTAHLHLLPARGRALDLAC
                     GLGANARVLAAAGLDTEAWDVSPVAIGRLQALAAAEGLRITATACDLLETPPAPQSFD
                     VIVVAYFFERALLPALAAALRPGGLLFYETFTQRSAHGPSNPDWKLADNELPSALPGL
                     RLRYYRDEQDAGDPALGLRGIAGYIGQRPA"
     gene            complement(996..2372)
                     /gene="trkA"
                     /locus_tag="PK144_11640"
     CDS             complement(996..2372)
                     /gene="trkA"
                     /locus_tag="PK144_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNF66427.1"
                     /translation="MKILILGAGQVGSSVAYNLASEDNDITVVDLRADVLADLQDRID
                     IRTVCGACSHPEILEAAGAADADMLIALTSSDEVNMIACAVADSLFRTPTKIARVRTA
                     EYTQYPQLFGAHGLPIEKLIAPEQLVTDHIQRLIEHPGALQVLDFADGLVRLVGVRAI
                     AGGTLVGKQLRTLPAHMPGIHARVAAIFRAGEAITPDGDTVIEADDEVFFIAAKKSIR
                     AVMAELRRLDKPVKRVILAGGGNIGRRLAHALEDRFQVKIIERNPEVARRIAEQLDRT
                     IVLAGDAADEELLLEENIEHTDVFCAVTNDDEANILSSMLAKRLGARKVMALINRPAY
                     VDLVEATGQIDVAISPQQATIGALLTHIRRGDVVRVHALRRGAAEAIEAIAHGDRQTS
                     KVVGRRIDEIRLPPGTTIGAVVRNGEVTICHHDTLIEPDDHVILFLTDKKRIHEVERL
                     FQVAVTFL"
     gene            complement(2423..3796)
                     /locus_tag="PK144_11645"
     CDS             complement(2423..3796)
                     /locus_tag="PK144_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF66428.1"
                     /translation="MSSAHILVVDDEPDIRDLVKEILEDENYEVSTAENGAAAREARR
                     ARRPDLILLDIWMPDIDGISLLKEWAEGDGLPCPVIMMSGHGTVETAVEATRLGAWDF
                     IEKPLSMAKLLLTLERALEADKLRRENAGLKRHARPVTEPVGRSETVQKLKAQAQRIA
                     QHEAPVLISGEPGTGKEIYARYVHAHSPRRGGPFVDVGVGSIARENSALELFGSEEGE
                     RLHYGRLEQASGGTLFLDELADMAPETQAKLAGALENGSFLRLGGREPVHLNVRVIAA
                     THRDLESEVAAGRFREDLYYHLNVLPIRLPPLREHCEDVPDLLSFYVNHFVDQESLPY
                     RNFTMAARNRLRNYGWPGNIRELKNLVQRLLILGGAEEITLEEVESAISHTTPGRQRD
                     ISQIPLDLPLREAREQFERAYLLQQFKECDGNVARLAEKVGMERTNLYRKLRALGIDP
                     KRTPEDE"
     gene            complement(3793..6006)
                     /locus_tag="PK144_11650"
     CDS             complement(3793..6006)
                     /locus_tag="PK144_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66429.1"
                     /translation="MSMRRIAVRGLLVNAALLAVLLVALALMGEATGNSARFGELYSW
                     LLVGSALGFVLLAFLIGRNLWDLYRQVRNDEPGSRLTLRLFVLFSLISVAPVSVVYYF
                     SVQFIARSIDSWFDVRVEKALEDALELSRTALDLRMREVLKQTVRLAGQLGETPGPPS
                     TLLLAELREESDAQELAVLKADGTPLVVAASEAASLVPERPPDVVLAQLRQGGNYVAL
                     DPTAELGFQLRAVVPVPAHATIGDDLLLQALYPVTDRFNSLGHSVQAAFDAYRELVFL
                     REPLRTTFIMTLSLVLLFGVLSALSAAVYFARKFVQPVRDLADGTRAVAAGQYDKQLA
                     PAANDELGFLVQSFNEMTRNLASARDSALQSRHMLEGQRAYLETVLARLSSGVLTLDH
                     DGHPRTFNAATLHILGLDAGHPLSAEGESPLAHLLGAVAGHLAGESAEWRQEVTLFAP
                     GGRRVLMCRGSRLPDAVGLKGGHVLVFDDITTLVQAERDAAWTEVARRLAHEIKNPLT
                     PIQLAAERIRHKYLGKLGEEEGRVLDRGTHTIVQQVAAMKEMVDAFNDYARAPQLRIV
                     PVPLNEFVTDVLYLYRDYPAGVEIQLCTDPAHPVIEADKNRLRQLLHNVVKNAIEAIR
                     DGHGSKLMISTRVHDGVELVFQDDGPGFPEGSLGNVFEPYVTTKPKGTGLGLAIVKKI
                     VEEHGGLIRAENAQQGGARITIRFPASHARTPLTPAPAAAPAPGAVPSTRAEEAG"
     gene            complement(6014..6589)
                     /locus_tag="PK144_11655"
     CDS             complement(6014..6589)
                     /locus_tag="PK144_11655"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNF66430.1"
                     /translation="MTAFTDRHRTPCLALLLGLLCLWLAPVPAAADGFRVLRASSTSE
                     RGLLRLDALIDLQFTEAPLDALRNGVPLTIEIDVEIVRDRDLLWDETVASLSQRFRLE
                     YHALARQYVAVNLNTNDLRSFPTLAGALDAIGRLHDFPLIDRALLKPPGNYYGRLRAS
                     LDIEALPPPLRPVAYLSTDWRLGSTWYAWSL"
     gene            complement(6586..>7938)
                     /gene="rsmB"
                     /locus_tag="PK144_11660"
     CDS             complement(6586..>7938)
                     /gene="rsmB"
                     /locus_tag="PK144_11660"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNF66431.1"
                     /translation="RGGERREPRPRAARRGPPLDARLAAAHALADVLDGRMSLNAALP
                     RYSERVVARDRGLAAELAYGTLRLYPRLALLLDQLLERPLAAGTGLVRGLLLCGLYQL
                     DHLRVPEYAAVASTVEAARALRHQPWAAGLVNAVLRNRQRRRAALDAALAAEPVAVHA
                     HPAWLLERLQADWPADWPAIVEASNQQAPMMLRVNLARESRDEALARLAAAGIAAAAH
                     AQVASAVVLEHPAEVSALPGFADGRLSVQDGAGQLAAPLLAPEPGDRVLDACCAPGGK
                     TGHLLETALGLELLALDIDATRLVRVRENLDRLGYDAELLAADAAAPADWWDGRPFER
                     ILLDAPCSATGVIRRHPDIKALRQPADIDALAARQRALLDALWPLLAPGGILLYATCS
                     LLRAENETVIAAFLAAHPDAAELVIDADWGLALRHGRQILPGSAGLDGFYYARLLRTP
                     "
BASE COUNT         1114 a         2656 c         2990 g         1178 t
ORIGIN      
        1 gccacacaca gggcgcctcc gggcgccctg tgcttttgtg gagggtcgtg cggcgggctg
       61 aggacgcggg caggaagcta cggggcgtcc ggtggcaggc tgcgcagcag gcgacggtgg
      121 aacaccgcca tctccttcgc ggcctgcagg tcgccgcggg cttcggcgac cgcgatgccc
      181 tgcgtccatg ccgcaagcgc accggccgga tcgccgagtg cggcgcgggc atggccgagc
      241 agcttccagg cggccgagta ctgcgcatcg agcccgaccg cgcgttcgag gtgggcatgt
      301 gcgcgcgccg cgtcgccggt gtcgaggtac agcttgccga gcgtcgtgcg cagcagggcg
      361 ccgtcctggc cgcgcgcgat catggcttcg agggcggcgg gattcatgcc gggcgctggc
      421 cgatgtagcc ggcgatgccg cgcaggccga gcgccgggtc gccggcgtcc tgctcgtcgc
      481 ggtaatagcg caggcgcagg ccgggcaggg ccgatggcag ttcgttgtcg gcgagcttcc
      541 agtcggggtt cgacgggccg tgggcgctgc gctgcgtgaa ggtctcataa aacagcaggc
      601 cgccggggcg cagcgccgcg gcgagtgccg gcagcagcgc gcgctcgaag aagtacgcga
      661 cgacgatcac gtcgaagctc tgcggagccg gtggcgtttc cagcaggtcg caggcggtgg
      721 cggtgatcct caggccttcg gcggcggcga gcgcctgcag tcgcccgatc gcgaccggcg
      781 acacgtccca agcctcggtg tcgagcccgg ccgcggccag cacgcgggcg ttggcaccga
      841 ggccgcaggc cagatccagc gcccggccgc gggccggcag caggtgcagg tgtgcggtca
      901 gcgtcgcgca ggggcgctgc tgcgggatgc cgtcgcggta acgggcatcc cagcgatcgc
      961 ggtcggtgac gggcatgcgg ggttcccggg gtggctcaga ggaaggtcac ggcgacctgg
     1021 aacaggcgct cgacctcgtg gatgcgcttc ttgtcggtca ggaacagaat cacgtgatcg
     1081 tcgggttcga tcagcgtgtc gtggtggcag atggtgacct cgccgttgcg cacgacggcg
     1141 ccgatggtcg tgccgggcgg caggcggatc tcgtcgatgc gccggccgac gaccttgctg
     1201 gtctggcggt cgccgtgggc gatggcctcg atggcttcgg ccgcaccgcg gcgcagcgca
     1261 tgcacgcgca cgacatcgcc gcggcggatg tgcgtcagca gcgcgccgat cgtcgcctgc
     1321 tgcggcgaga tcgccacgtc gatctggccg gtggcctcga ccaggtcgac gtaggccggg
     1381 cggttgatca gcgccatgac cttgcgggcg ccgaggcgct tggcgagcat cgacgacagg
     1441 atgttggcct cgtcgtcatt ggtcacggcg cagaacacgt cggtgtgctc gatgttctct
     1501 tcgagcagca gctcctcgtc ggcggcatcg ccggcgagca cgatcgtgcg gtcgagctgt
     1561 tcggcgatgc ggcgtgcgac ctcggggttg cgctcgatga tcttgacctg gaagcggtcc
     1621 tccagcgcgt gcgcgagccg ccggccgatg ttgccgccgc cggcgaggat cacgcgcttg
     1681 accggcttgt cgagcctgcg cagctccgcc atcaccgcgc ggatgctctt cttcgcggcg
     1741 atgaagaaca cctcgtcgtc ggcctcgatc accgtgtcgc cgtcgggcgt gatcgcctcg
     1801 ccggcgcgga agatcgccgc gacgcgcgcg tggatgccgg gcatgtgtgc cggcagcgtg
     1861 cgcagctgct tgccgaccag cgtgccgccg gcaatcgcgc gcacgccgac cagacgcacg
     1921 aggccgtcgg cgaagtccag cacctgcagc gcgccggggt gctcgatcag gcgctggatg
     1981 tggtcggtga cgagctgctc gggcgcgatc agcttctcga tcggcaggcc gtgcgcgccg
     2041 aacagctgcg ggtactgcgt gtactcggcg gtgcgcacgc gcgcgatctt ggtcggcgtg
     2101 cggaacaggc tgtcggcgac cgcgcaggcg atcatgttga cctcgtcgct gctggtcagc
     2161 gcgatcagca tgtcggcatc ggcggctccg gcggcttcga ggatctccgg gtgcgaacag
     2221 gcgccgcaga cggtgcggat gtcgatgcgg tcctgcaggt cggcgagcac gtcggcgcgc
     2281 aggtcgacga cggtgatgtc gttgtcctcg gaagcgaggt tgtacgcgac cgagctgccg
     2341 acctggccgg caccgaggat caggatcttc acgatgtcct cacggaatcc tcacgacggc
     2401 accgggctgc ttcgtgcccg gctcattcgt cctcgggcgt gcgcttcgga tcgatgccga
     2461 gggcgcgcag cttgcggtac aggttcgtgc gctccatgcc gaccttctcg gcgaggcgcg
     2521 cgacgttgcc gtcgcattcc ttgaactgct gcagcaggta ggcgcgctcg aactgctcgc
     2581 gcgcctcgcg cagcggcagg tcgagcggaa tctggctgat gtcgcgctgg cgcccgggcg
     2641 tcgtgtgcga gatcgccgat tcgacctctt cgagcgtgat ctcctcggcg ccgccgagga
     2701 tcagcaggcg ctgcacgagg ttcttcagtt cgcggatgtt gccgggccag ccgtagttgc
     2761 gcaggcggtt gcgcgccgcc atcgtgaagt tgcggtacgg caggctttcc tgatcgacaa
     2821 aatgattgac gtagaacgac agcaggtcgg gcacgtcctc gcagtgctcg cgcagcggcg
     2881 gcagccggat cggcagcacg ttcaggtggt agtagagatc ctcgcggaag cgcccggccg
     2941 cgacctcgga ttcgaggtcg cgatgcgtcg cggcgatcac gcgcacgttc aggtgcaccg
     3001 gctcgcggcc gccgaggcgc aggaaggagc cgttctcgag cgcgccggcg agcttggcct
     3061 gggtctcggg cgccatgtcg gcgagctcgt cgaggaacag cgtgccgccg ctcgcctgtt
     3121 cgagccggcc gtaatgcagg cgctcgcctt cctcggagcc gaacagctcc agcgccgagt
     3181 tctcgcgcgc gatcgagccg acgccgacat cgacgaacgg cccgccgcgg cgcgggctgt
     3241 gcgcatgcac gtagcgcgcg tagatctcct tgccggtgcc gggctcgccg gagatcagca
     3301 ccggtgcctc gtgctgggcg atgcgctggg cctgggcctt gagcttctgc acggtctcgc
     3361 tgcgcccgac cggctcggtc accggccgcg cgtggcgctt gaggccggcg ttctcgcggc
     3421 gcagcttgtc ggcttcgagc gcgcgttcga gcgtcagcag cagcttggcc atcgacagcg
     3481 gcttctcgat gaagtcccag gcgccgaggc gcgtggcctc gaccgcggtc tcgaccgtgc
     3541 cgtggccgga catcatgatc accgggcagg gcaggccgtc gccctcggcc cattccttga
     3601 gcaggctgat gccgtcgatg tccggcatcc agatgtcgag caggatcagg tccgggcgcc
     3661 gcgcacggcg ggcctcgcgg gcggcggcgc cgttttccgc ggtcgagacc tcgtagttct
     3721 cgtcttcgag gatttccttg accagatcgc ggatgtcggg ctcgtcgtcg acgacgagga
     3781 tgtgcgctga actcatcctg cctcctcggc acgggtcgat ggcacggcac ccggcgccgg
     3841 tgcggcggca ggtgcgggtg tcagtggggt gcgggcgtga ctggcgggaa agcggatggt
     3901 gatgcgagcg ccgccctgct gggcattctc cgcacggatc aggccgccgt gctcttcaac
     3961 gatttttttg acgatcgcca atcctagtcc cgtgcccttg ggtttggtag tcacgtaggg
     4021 ttcgaacacg ttgccgaggc tgccctcggg aaagccgggg ccgtcatcct ggaagaccag
     4081 ctcgacgccg tcgtgcacgc gcgtcgagat catcagcttc gagccgtggc cgtcgcggat
     4141 ggcttcgatg gcgttcttga cgacgttgtg cagcagctgc cgcagccggt tcttgtcggc
     4201 ctcgatgacc gggtgcgccg ggtccgtgca gagctggatc tccacgccgg ccgggtagtc
     4261 gcgatacaga tagagcacgt cggtgacgaa ctcgttcaac ggcaccggca cgatgcgcaa
     4321 ctgcggcgcg cgcgcgtagt cgttgaacgc atcgaccatc tccttcatcg ccgcgacctg
     4381 ctgcacgatc gtgtgcgtgc cgcgatcgag cacgcggcct tcctcctcgc ccagcttgcc
     4441 gaggtatttg tggcggatgc gctcggcggc gagctggatc ggcgtcagcg ggttcttgat
     4501 ctcgtgcgcg aggcggcgcg cgacctcggt ccaggcggcg tcgcgctcgg cctgcaccag
     4561 cgtcgtgatg tcgtcgaaca ccagcacgtg cccgcccttg aggccgacgg catccggcag
     4621 ccgcgagccg cggcacatca gcacgcgccg cccgccgggg gcgaacagcg tgacctcctg
     4681 ccgccattcg gcgctctcgc cggcgagatg tccggcgacg gcgccgagca ggtgcgcgag
     4741 cggcgattcg ccctcggccg acagcgggtg gccggcatcg aggccgagga tgtgcagcgt
     4801 cgcggcgttg aaggtgcgcg ggtggccgtc gtgatcgagc gtcagcacgc ccgaggacag
     4861 gcgcgcgagc acggtttcga ggtaggcgcg ctgaccttcg agcatgtgcc ggctctgcag
     4921 cgccgagtcg cgcgcgctgg cgaggttgcg cgtcatctcg ttgaaggact gcacgaggaa
     4981 gccgagctcg tcgttggccg ccggcgcgag ctgcttgtcg tactggccgg cggcgaccgc
     5041 gcgcgtgccg tcggcgaggt cgcgcaccgg ctgcacgaac ttgcgcgcga agtacacggc
     5101 cgccgacagc gcactcagca cgccgaacag cagcaccagc gacagcgtca tgatgaaggt
     5161 cgtgcgcagc ggctcgcgca ggaagaccag ttcgcggtag gcatcgaagg ccgcctgcac
     5221 cgaatgcccg agcgagttga agcggtcggt gaccgggtac agcgcctgca gcagcaggtc
     5281 atcgccgatg gtcgcgtgcg ccggcaccgg cacgaccgcg cgcagctgga agccgagttc
     5341 ggcggtcggg tcgagcgcga cgtagttgcc gccctggcgc agctgcgcga gcacgacatc
     5401 cggcgggcgc tccggcacca ggcttgcggc ctcgctggcg gcgaccacca gcggcgtgcc
     5461 gtcggccttc agcacggcca gctcctgcgc atcgctctcc tcgcgcagct ccgcgagcag
     5521 cagcgtcgac ggcgggcccg gggtctcgcc gagctggccg gcgaggcgca cggtctgctt
     5581 gagcacctcg cgcatgcgca ggtcgagcgc ggtgcgcgac agttcgaggg catcctccag
     5641 cgccttctcg acgcgcacgt cgaaccacga atcgatcgag cgcgcgatga actgcaccga
     5701 gaagtaatac acgaccgaca ccggcgcgac cgagatcagc gagaacagca cgaacagccg
     5761 cagcgtcagc cgcgagccgg gttcgtcgtt gcgcacctgc cggtacagat cccagaggtt
     5821 gcgcccgatc aggaacgcga gcagcacgaa gccgagcgcg ctgccgacca gcagccacga
     5881 gtacagctcg ccgaagcgcg cggagttgcc ggtcgcctcg cccatcagcg ccagcgccac
     5941 cagcagcacc gccagcagcg ccgcgttgac cagcaggccg cgcactgcaa tccgccgcat
     6001 gctcatgacg acctcatagc gaccacgcgt accaggtcga gccgagccgc cagtccgtcg
     6061 acaggtaggc gaccgggcgc agcggtggcg gcagcgcctc gatgtcgagg ctcgcgcgca
     6121 gccgcccgta atagttgccg gggggcttga gcagcgcccg gtcgatcagc gggaagtcgt
     6181 gcagccggcc gatggcatcg agcgcgccgg caagcgtcgg gaagctgcgc aggtcattgg
     6241 tgttgaggtt cacggccacg tactggcgcg cgagcgcgtg gtattcgagc cggaagcgct
     6301 ggctgaggct ggcgacggtc tcgtcccaca gcagatcgcg gtcgcggacg atctcgacat
     6361 cgatctcgat ggtgagcggc acgccgttgc gcagcgcatc gagcggggcc tcggtgaact
     6421 gcaggtcgat cagcgcgtcg aggcgcagca ggccgcgctc gctggtcgaa ctggcgcgca
     6481 gcacgcggaa gccgtcggcg gcggccggca ccggcgcgag ccacagacac agcagcccga
     6541 gcagcagcgc gaggcacggg gtccggtggc ggtcggtgaa agcggtcacg gggtcctgag
     6601 caggcgtgcg tagtagaagc cgtcgaggcc ggcgctgccc ggcaggatct ggcggccgtg
     6661 gcgcagcgcg agcccccagt ccgcgtcgat gacgagttcc gcggcatcgg ggtgggcggc
     6721 gaggaaagcc gcgatcacgg tctcgttttc ggcgcgcagc agcgagcagg tggcgtacag
     6781 caggataccg cccggggcca gcagcggcca cagcgcatcg agcagcgcgc gctggcgggc
     6841 ggcgagcgcg tcgatgtcgg ccggctggcg cagcgccttg atgtcgggat ggcggcggat
     6901 cacgccggtg gccgagcacg gcgcatcgag caggatgcgc tcgaagggcc ggccgtccca
     6961 ccagtctgcc ggtgccgcgg catcggcggc gagcagttcg gcgtcgtagc cgagccggtc
     7021 gaggttctcg cgcacgcgca cgaggcgcgt ggcatcgatg tcgagcgcca gcagttcgag
     7081 gccgagtgcg gtttcgagca ggtggccggt cttgccgccg ggcgcgcagc aggcatcgag
     7141 cacgcgatcg cccggctcgg gcgcgagcag cggcgcggcg agctggccgg cgccgtcctg
     7201 caccgacagc cggccatcgg cgaagccggg cagggcgctg acctcggcgg ggtgctccag
     7261 cacgacggca ctcgccacct gcgcgtgtgc cgcggcggcg atgccggccg ccgcgagccg
     7321 cgccagtgcc tcgtcgcggc tttcgcgcgc caggttcacg cgcagcatca tcggggcctg
     7381 ctggttgctg gcctcgacga tcgccggcca gtccgcgggc cagtcggcct gcagccgctc
     7441 cagcagccag gccggatgcg cgtgcacggc caccggctcg gcggccagtg cggcatcgag
     7501 tgccgcgcgc cggcgctggc ggttgcgcag cacggcgttg acgaggccgg ccgcccaggg
     7561 ctggtgtctg agtgcgcgcg ccgcctcgac cgtgctggcg acggccgcgt actcgggcac
     7621 gcgcaggtga tcgagctggt acagcccgca gagcagcagg ccgcgcacga ggccggtgcc
     7681 ggcagcgagc ggccgttcga gcagctgatc gagcagcagc gcgaggcgcg gatacagccg
     7741 cagcgtgccg taggcgagtt cggcggccag accgcggtcg cgcgccacga cgcgctcgct
     7801 gtagcgcggc agtgcggcat tcagcgacat gcggccgtcg agcacgtcgg cgagcgcgtg
     7861 cgcggcagcg aggcgcgcat cgagcggcgg accgcggcgg gcagcgcggg gccgcggttc
     7921 gcggcgctcg ccgccgcg
//
LOCUS       DAOQIE010000180         7914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4733_length_7914_cov_10.109301, whole genome shotgun sequence.
ACCESSION   DAOQIE010000180 DAOQIE010000000
VERSION     DAOQIE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_7914_cov_10.109301"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..633
                     /locus_tag="PK144_11665"
     CDS             172..633
                     /locus_tag="PK144_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF66432.1"
                     /translation="MNMPVRGRGIALGIIALGFATTAAAGSAVGSTMVLAAAPTEMPV
                     IQQSGTIRYLSGGVGDEERTAMNQVENQFNLGITLATANGSFLSDAQIRISDHKGSTV
                     LETVPNGPMLFVQLPPGEYKVDAQGFGQHYSKTVHVGERMSKITFTWPAQG"
     gene            complement(733..1221)
                     /gene="bfr"
                     /locus_tag="PK144_11670"
     CDS             complement(733..1221)
                     /gene="bfr"
                     /locus_tag="PK144_11670"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNF66433.1"
                     /translation="MQGNPQIIATLNQLLTGELSAADQYLYHAHRYLDWGLHALWERT
                     HHEMEEERQHAQVLIERILFLEGRPDVASRDPLALGSDVESMLKNDLALEYQVVGALR
                     AAIAQCEQLGDYQTRNELLPLLADTEEDHAHWLEQQLGLIDRIGLENYLQSQMGQAPS
                     AS"
     gene            complement(1323..2039)
                     /locus_tag="PK144_11675"
     CDS             complement(1323..2039)
                     /locus_tag="PK144_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNF66434.1"
                     /translation="MNDLFQLWVYLAASPLLWLLVTVLAYLTGVWLFRISKANPLFTP
                     VLVGVVIVVSVLLVTGTPYPVYFEGAKFVHFLIGPATVALAVPLYSVFGRLKTMLRPL
                     LIALFAGALTSIGSAIGIAWALGASRATLMSLAPKSVTMPIAMGVAERIGGIPSLAAV
                     AVAVTGISGAMMARGLLNRLRIEDMAVRGFAAGLAAHAVGTARAHQIHPDAGAFAALA
                     MGLNGIVTALLLPLLVRLSG"
     gene            complement(2036..2419)
                     /locus_tag="PK144_11680"
     CDS             complement(2036..2419)
                     /locus_tag="PK144_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNF66435.1"
                     /translation="MIQAFAVLLFFQFLGEVGVQGFGLPFPGPLLGMLLLFVALLVHG
                     RVPEGLRALSQSLIGNLMLLFIPAVSGVMIHWQKVAADGVAFVVSGVLATALTLVVTA
                     LLLKHLLARRAHGDADNGPDAGTPS"
     gene            2622..4229
                     /locus_tag="PK144_11685"
     CDS             2622..4229
                     /locus_tag="PK144_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNF66436.1"
                     /translation="MSTLHSSLNPRAGEFRRNAEAMAALVADLRAQVADIARGGGDAA
                     MRRHVARGKLPVRERIRTLLDVGSPFLECSQLAAHGVYAEPVPAAGLVTGIGRIHGRE
                     CVIVANDATVKGGTYYPLTVKKHLRAQEIARENHLPCVYLVDSGGAHLPNQDEVFPDR
                     EHFGRIFYNQATLSALGIAQIAVVMGSCTAGGAYVPAMADESIIVRAQGTIFLAGPPL
                     VRAATGEVVSAEELGGADVHCRRSGVTDHYALDDAHALGLARRAVANLNRVKPVGLAL
                     LPARAPLHPPEELHGVIPADPRQPYDVREVIARLVDASEFDEFKALYGPTLVTGFAHL
                     YGYPVGIVASNGVLFSEAALKGAHFVELCCQRGIPLVFLQNITGFMVGRRYEAGGIAK
                     DGAKMVTAVACAQVPKFTVLIGGSFGAGNYGMCGRAYGPRFLWMWPNARISVMGGEQA
                     AGVLAQVKREAIERDGGQWSAEDEDAFKAPIRAQYETQGHPYYASARLWDDGIIDPAD
                     TRRVLGLGISAALNRPIERTGFGVFRM"
     gene            4296..4598
                     /locus_tag="PK144_11690"
     CDS             4296..4598
                     /locus_tag="PK144_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF66437.1"
                     /translation="MYTVLRSSDFDAWLSALNDHTGKARILSRIDSAERGNLGDCASV
                     GDGVHEMRVHVGPGYRVYFTIRGRLMIFLLLGGDKSTQKKDIRRAKAIVKLLGDAP"
     gene            4595..4882
                     /locus_tag="PK144_11695"
     CDS             4595..4882
                     /locus_tag="PK144_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNF66438.1"
                     /translation="MTTFSRYDATDYLDNEEVIAEYLSAALEDPDPDVLLAALANVAK
                     ARGIAQLARETGLGRESLYKALAPGAKPRYETIAKLLNALGVRLQAHTLNS"
     gene            4913..5695
                     /locus_tag="PK144_11700"
     CDS             4913..5695
                     /locus_tag="PK144_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNF66439.1"
                     /translation="MAIEASLTTRHVAIVTLNRPELHNAFDDALIAELTAELRRLDAD
                     DSVRIVVLAAAGKSFSAGADLHWMRRMAAYDEAQNLADARALAELMRTLDGLSKPVIA
                     QVQGAAYGGGVGLIACCDVVIAAETASFCLSETRLGLIPAVISPYVVAAIGARQARRW
                     CVSAEAFDAATALRLGLVHEVVAPGRLAARVDELLATLAGNGPQAMREAKRLVRDVSR
                     RPLDAALIDDTARRIARLRASPEGREGVSAFLDKRAPAWRDG"
     assembly_gap    5696..5795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5797..7827
                     /locus_tag="PK144_11705"
     CDS             5797..7827
                     /locus_tag="PK144_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNF66440.1"
                     /translation="MFDKILIANRGEIACRVIASCRRLGIRSVAVYSDADAQALHVGL
                     ADEAVRIGPAKAADSYLCAARIVGAALASGAQAVHPGYGFLSENAAFAEAVEAAGLVF
                     IGPPPAAIRAMGSKSAAKVRMAQAGVPVVPGYHGEDQSLERLTAEAIATGFPLLLKPS
                     AGGGGKGMKVVTHAGELGAQIESARREAAASFGDEQLLIERYLERPRHIEIQIFADAH
                     GRCVSLFERDCSLQRRHQKVIEEAPAPGLDPARRQAMGEAACTAALAIGYRGAGTVEF
                     IAEHEEAGDGRFFFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPLAQAQIPL
                     HGHAIEARIYAEDPARDFLPASGRLVHLHWPAGDAHVRIDTGVRQGDAVTPHYDPLLA
                     KLIVHDEDRPRALARLRRALADCRIVGVANNLRFLAALAAHPGYAAGAVHTGFIADQR
                     ATLLPAPATAPMRVLALATLGRLLKAARDAARRAAASAEPDSPWQHGDGWRLNEDNHQ
                     VLRYRDGDDGPVQAVIAHYRPGHWRLELPDGSTATVAGRLDDEDVVHAEIDGRRTRAT
                     LVRTGAQLHVLGLDGAWTLTLHDPLAQAMAGEAAGAGRLSAPMPGTVVAVRVAPGEAV
                     EAGQVLLILEAMKMEHMLRAPRAGTVASIACTVGETVTEGTELLKLAGEEEG"
BASE COUNT         1260 a         2660 c         2736 g         1158 t
ORIGIN      
        1 gggaccggat gcagccgcaa cttgccacgt ggcaagtccg gtgccctcct gatcagcgat
       61 gtaccgggtt ttcaggtact tgcgtcggct gccggaaccc ggcgcaccgc gggtggtcga
      121 tgcaggtgct cgccctgatg ggcctttgcc atccccccgg aggaagccat catgaacatg
      181 cctgttcgcg gccgcggtat cgctctcggc atcatcgccc tcggtttcgc caccacggct
      241 gcagccggtt cggcggtcgg ctcgaccatg gtgctggcgg cagcgccgac cgaaatgccg
      301 gtgatccagc agtccggcac gatccgctac ctgagcggcg gtgtcggcga tgaagaacgc
      361 accgcgatga atcaggtcga gaaccagttc aatctcggca tcacgctggc gacggccaac
      421 ggttcgttcc tgagcgatgc gcagatccgc atcagcgacc acaagggttc gaccgtgctc
      481 gaaaccgtgc cgaacggccc gatgctgttc gtgcagctgc cgcccggcga atacaaggtc
      541 gatgcccagg gcttcggcca gcactactcg aagaccgtcc acgtcggcga acgcatgagc
      601 aagatcacgt tcacgtggcc ggcgcagggc tgatcatcgc ccgcatcgac tgaaagcctg
      661 aaaagccagc gccccgaccg tctgctgcgg tcggggcgct ggcttttcag gggcgatgga
      721 aaaaccgcgg gatcagctcg ccgatggtgc ctggcccatc tgcgactgca ggtagttctc
      781 cagcccgatc cggtcgatca ggccgagctg ctgctccagc cagtgcgcgt ggtcttcctc
      841 ggtgtcggcg agcagcggca gcagctcgtt gcgcgtctgg tagtcgccga gctgctcgca
      901 ctgcgcgatc gcggcgcgca gggcgccgac cacctggtat tcgagcgcga ggtcgttctt
      961 cagcatcgac tcgacatcgc tgccgagcgc gagcggatcg cggctggcga catcgggcct
     1021 gccttcgagg aacaggatgc gttcgatcag cacctgcgcg tgctggcgtt cctcttccat
     1081 ctcgtggtgc gtgcgctccc agagcgcgtg caggccccag tcgaggtagc ggtgcgcgtg
     1141 gtacaggtac tggtcggccg cgctcagttc gccggtcagc agctggttca gggtggcgat
     1201 gatctgcggg ttgccttgca tcgttgcggg cctttgacag gaacagggaa gggacggacc
     1261 gcgcctcaat aacgcgatcg gccgtatttg tcatccgttc tcatttgtcc ggccgcggtc
     1321 gttcagccgg acagccgcac cagcaacggc agcagcagcg cggtgacgat gccgttcagc
     1381 cccatcgcca gcgccgcgaa ggcaccggcg tccgggtgga tctggtgggc gcgggcggtg
     1441 ccgaccgcgt gtgccgccag tccggcggcg aagccgcgca cggccatgtc ctcgatgcgc
     1501 aggcggttca gcaggccgcg cgccatcatc gcgccggaga tgccggtcac cgccaccgcc
     1561 accgccgcca gcgacgggat gccgccgatg cgctcggcca cacccatcgc gatcggcatc
     1621 gtcaccgact tcggcgccag cgacatcagc gtcgcgcgcg aggcaccgag cgcccaggcg
     1681 atgccgatgg cactgccgat cgaggtcagc gcgccggcga acagcgcgat cagcagcggc
     1741 cggagcatcg tcttcagccg gccgaacacg ctgtacagcg gtacggccag cgccacggtc
     1801 gccgggccga tcaggaagtg cacgaacttc gcgccctcga agtacaccgg atacggcgtg
     1861 ccggtcacga gcagcaccga aaccacgatg acgacgccga ccagcaccgg cgtgaacagc
     1921 ggattggcct tgctgatgcg aaacagccag acgccggtca gatacgcgag cacggtcacg
     1981 agcagccaca gcagcggcga ggcggccaga tacacccaga gctggaacag gtcgttcatg
     2041 acggcgtacc cgcatccgga ccgttatccg cgtcgccgtg cgcccgtcgg gccagcagat
     2101 gcttcagcag cagcgcggtc acgaccagcg tcagcgccgt cgccagcacg ccggagacga
     2161 cgaaggccac gccgtcggcg gcgaccttct gccagtggat catcacgccg ctgaccgccg
     2221 ggatgaacag cagcatcagg ttgccgatca gcgactgcga cagtgcccgc agcccttcgg
     2281 gcacgcgccc gtgcacgagc agcgccacga acagcagcag catgccgagc agcggccccg
     2341 gaaacggcag gccgaagccc tgcacaccga cctcgccgag gaactggaag aacagcagca
     2401 cggcaaacgc ctggatcatc gcgaacaact cctcggccgt gacacgccgg ccgggcaggg
     2461 gggaaggaac gccgggggga tcagcgtggt gttgcgctta tagccagctc cggccggcgg
     2521 acgcatgcgg cacatgccag ccagacgccg atgcaccagc ggcaggcggc tgacgcgtgc
     2581 gtcaactaga ttcgcgccgc cgtcccgccc ggagcctgcc gatgagcacg ctgcattcga
     2641 gcctgaaccc gcgcgccggg gagttccgcc gcaatgccga agccatggcc gcgctggtcg
     2701 ccgacctgcg cgcgcaggtc gcggacatcg cgcgcggcgg cggcgatgcg gcgatgcgcc
     2761 ggcacgtcgc gcgcggcaag ctgccggtgc gcgagcgcat ccgcacgctg ctcgacgtcg
     2821 gctcgccgtt cctggagtgc tcgcagctcg ccgcgcacgg ggtgtacgcc gagccggtgc
     2881 cggccgccgg gctcgtcacc ggcatcggcc gcatccacgg ccgcgaatgc gtgatcgtcg
     2941 ccaacgacgc cacggtcaag ggcggcacct actacccgct gacggtgaag aagcacctgc
     3001 gcgcgcagga gatcgcgcgc gagaaccatc tgccctgcgt gtacctggtc gattccggcg
     3061 gtgcgcacct gccgaaccag gacgaggtct ttccggatcg cgagcacttc ggccgcatct
     3121 tctacaacca ggcgacgctc tcggcgctcg gcatcgcgca gattgccgtg gtcatgggct
     3181 cgtgcacggc cggcggtgcc tacgtgccgg cgatggccga cgaatcgatc atcgtgcgtg
     3241 ctcagggcac gatcttcctg gccggcccgc cgctcgtgcg cgccgcgacc ggcgaggtgg
     3301 tcagtgccga ggaactcggc ggtgccgacg tgcactgccg ccgctcgggc gtcaccgatc
     3361 actacgcgct cgacgatgcg cacgcgctcg gtctggcgcg gcgcgcggtc gcgaacctga
     3421 accgcgtcaa gcccgtcgga ctcgcgctgc tgccggcgcg cgcgccgctg catccgcccg
     3481 aagagctgca cggcgtgatc ccggccgatc cgcgccagcc ctacgacgtg cgcgaggtca
     3541 tcgcgcgcct cgtcgatgcc agcgagttcg acgagttcaa ggcgctgtac ggcccgacgc
     3601 tggtcaccgg cttcgcgcac ctgtacggct atccggtcgg catcgtcgcc agcaacggcg
     3661 tgctgttctc ggaggcggcg ctgaagggcg cgcacttcgt cgaactctgc tgccagcgcg
     3721 gcatcccgct ggtgttcctg cagaacatca ccggtttcat ggtcgggcgc cgctacgagg
     3781 ccggcggcat cgccaaggac ggcgcgaaga tggtcaccgc cgtcgcctgc gcgcaggtgc
     3841 cgaagttcac ggtgctgatc ggcggcagct tcggggccgg caactacggc atgtgcgggc
     3901 gcgcctacgg gccgcgcttc ctgtggatgt ggccgaacgc gcgcatcagc gtcatgggcg
     3961 gcgagcaggc cgccggcgtg ctcgcgcagg tgaagcgcga ggcgatcgaa cgcgacggcg
     4021 gccagtggag cgccgaagac gaggatgcct tcaaggcgcc gatccgcgcg cagtacgaaa
     4081 cccagggcca cccgtactac gccagcgccc ggctctggga cgacggcatc atcgatccgg
     4141 ccgacacgcg ccgggtgctc ggcctcggca tttcggcggc gctgaaccgg ccgatcgaaa
     4201 ggacggggtt cggggtgttc cggatgtgag gcggcaagcg gacggaatca agaagcggct
     4261 tgtcagcctg tatccagtcg gatacggttg acgccatgta caccgtgctc agatccagcg
     4321 atttcgatgc gtggctttcc gctctcaatg accataccgg caaagcgcgg attctgtccc
     4381 gtatcgattc agccgaacgc ggcaatctcg gcgactgcgc atcggtcggc gatggcgtac
     4441 atgagatgcg cgtgcatgtc ggacccggct atcgggtcta tttcacgata cgtggccgac
     4501 tgatgatctt cctgctgctc ggtggcgaca agtcaacgca gaaaaaggat atcaggcgcg
     4561 ccaaggctat tgtcaaactg ctcggggacg cgccatgact acattttcca gatatgatgc
     4621 aacggattat ctcgacaatg aggaagtcat tgccgaatat ctgtcggctg ccctggagga
     4681 cccagacccc gacgtactgt tggccgcact ggcaaatgtt gccaaggcgc gcggcattgc
     4741 tcaattggcc agggaaaccg gtctcggtcg ggaaagtctg tacaaggcgc ttgctcccgg
     4801 cgcaaaacca agatacgaaa cgatagccaa gttgttgaat gcactcgggg taagattgca
     4861 ggcgcacacc ctgaattcct gaagttccgc ccggatgcat ggcgtgaatc gtatggctat
     4921 tgaagctagt ttgactacaa ggcatgtcgc aatcgttact cttaatcgtc cagaactgca
     4981 taacgctttc gacgacgcgc tgatcgccga gctgacggcg gaattacggc gcctcgacgc
     5041 cgatgattcg gtccgcatcg tcgtgctcgc cgcggccggc aagagctttt cagccggcgc
     5101 cgacctgcac tggatgcggc gcatggctgc ttacgacgag gcgcagaacc tcgctgacgc
     5161 ccgcgcgctg gccgaactga tgcgcacgct cgatggcctg tcgaagccgg tcatcgctca
     5221 ggtgcagggc gcggcgtatg gcggcggtgt cgggctgatc gcctgctgcg atgtcgtgat
     5281 cgccgccgaa accgcgagtt tctgtctgtc cgaaacccgg ctcgggctga tcccggcggt
     5341 gatctcgccg tacgtggtcg ccgcgatcgg cgcgcgtcag gcacggcgct ggtgcgtcag
     5401 cgccgaagcc ttcgacgccg cgaccgcgct gcggctcggg ctggtgcacg aagtggtcgc
     5461 gcccggacgg ctggcggcgc gcgtcgatga actgctggcg acgctcgccg gcaacggtcc
     5521 gcaggcgatg cgcgaagcca agcggctggt gcgcgacgtg agccgccgcc cgctcgacgc
     5581 ggcgctgatc gacgacaccg cccggcgcat cgcccgcctg cgtgcaagcc ccgagggccg
     5641 cgaaggggtt tcggcgtttc tcgacaagcg ggcgccggcg tggcgtgacg gctgannnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncatgt tcgacaagat cctcatcgcc
     5821 aaccgcggcg aaatcgcctg ccgggtgatc gcgagctgcc ggcggctcgg catccgcagc
     5881 gtcgccgtgt attcggatgc cgatgcgcag gcgctgcacg tcgggcttgc cgacgaggcc
     5941 gtgcgcatcg ggccggcgaa ggcggccgac agctatctgt gtgccgcgcg catcgtcggg
     6001 gccgcgctcg cgagcggtgc gcaggccgtg catccgggct acggcttcct gtccgagaac
     6061 gcggcgttcg ccgaggcggt cgaggccgcc gggctcgtgt tcatcgggcc gccgccggcg
     6121 gcgatccggg cgatgggctc gaagagcgcg gccaaggtgc gcatggcgca ggccggcgtg
     6181 ccggtggtgc cgggctatca cggcgaggat cagtcgctcg aacggctcac ggccgaagcc
     6241 atcgccaccg gctttccgct gctgctgaag ccgagcgccg gcggcggcgg caagggcatg
     6301 aaggtcgtca cgcacgccgg cgagctcggt gcgcagatcg agtcggcgcg gcgcgaggcg
     6361 gcggcgagct ttggcgatga gcagctgctg atcgagcgct acctcgaacg cccgcgccac
     6421 atcgagatcc agatcttcgc cgatgcccac ggccgctgcg tgtcgctgtt cgagcgcgac
     6481 tgctcgctgc agcgccggca ccagaaggtc atcgaggaag ccccggcccc gggcctcgat
     6541 ccggcgcgcc ggcaggcgat gggggaggcg gcctgcacgg cggcgctggc gatcggctat
     6601 cgcggcgccg gcaccgtcga gttcatcgcc gagcacgagg aagccggcga cgggcggttc
     6661 ttcttcatgg agatgaacac gcgcctgcag gtcgagcatc cggtcacgga aatgatcacc
     6721 ggcctcgatc tggtcgaatg gcagctgcgt gtcgccgccg gcgaaccgct gccactcgcg
     6781 caggcgcaga tcccgctgca cggccacgcg atcgaggcgc gcatctacgc cgaggatccg
     6841 gcgcgcgact tcctgccggc gagcggccgg ctcgtgcacc tgcactggcc ggcgggcgat
     6901 gcgcacgtgc gcatcgacac cggtgtgcgc cagggcgatg cggtcacgcc gcactacgac
     6961 ccgctgctcg cgaagctgat cgtccacgac gaggaccgcc cgcgcgcgct ggcgcggctg
     7021 cggcgggcgc tcgccgactg ccgcatcgtc ggggtggcga acaacctgcg cttcctcgcc
     7081 gcgctcgcgg cccaccccgg atacgcggcc ggcgccgtgc ataccggctt catcgccgac
     7141 cagcgcgcga ccctgctgcc ggcgccggcc acggcgccga tgcgcgtgct ggcgctggcg
     7201 acgctcgggc ggctgctgaa ggccgcgcgc gatgccgcgc gccgcgccgc cgccagcgcc
     7261 gagccggatt cgccgtggca gcacggcgac ggctggcggc tcaacgagga caaccatcag
     7321 gtgctgcgtt atcgcgatgg cgacgatggc ccggtccagg ccgtgatcgc gcactaccgg
     7381 cccgggcact ggcggctgga actgccggat ggctcgacgg cgacggtcgc cggccggctc
     7441 gatgacgagg atgtcgtgca cgccgaaatc gacggccggc gcacgcgcgc gacgctggtg
     7501 cgcaccgggg cacagctgca cgtgctcggc ctcgacggcg cctggacgct gacgctgcac
     7561 gatccgctgg cgcaggccat ggccggcgag gcggccgggg ccggacgcct gagcgccccg
     7621 atgccgggca cggtggtcgc ggtgcgggtc gcgccgggcg aggcggtcga ggccgggcag
     7681 gtattgctga tcctcgaagc gatgaagatg gaacacatgc tgcgcgcccc gcgcgccggc
     7741 acggtcgcca gcatcgcctg caccgtgggc gagacggtca cggaaggcac ggagctgctg
     7801 aagctggccg gcgaggaaga gggctgaacg gcggtgtgcc cgtgcctgcc gcggcgcact
     7861 cagccgccgg cccggctcag cgcgaacgcg ctcagaaaac cgagcacggt gatc
//
LOCUS       DAOQIE010000181         7892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4756_length_7892_cov_7.809749, whole genome shotgun sequence.
ACCESSION   DAOQIE010000181 DAOQIE010000000
VERSION     DAOQIE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4756_length_7892_cov_7.809749"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK144_11710"
     CDS             complement(<1..211)
                     /locus_tag="PK144_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNF66441.1"
                     /translation="MDETLLARLGACTHLPTLPAVAMRIIELGDDPDAGIGEIADVVC
                     RDPALTAKLLKIANSPLYAQRRKVEN"
     gene            complement(457..1470)
                     /gene="ilvC"
                     /locus_tag="PK144_11715"
     CDS             complement(457..1470)
                     /gene="ilvC"
                     /locus_tag="PK144_11715"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNF66442.1"
                     /translation="MNVYYDKDADLSLIQGKTVAIIGYGSQGHAHANNLSESGVQVVV
                     GLRAGGASWQKAVNAGLSVKEVDAAVEGADVVMILLPDENIAGVYQQIEKKIKQGAAL
                     AFAHGFNIHYNQVVPRADLDVIMIAPKAPGHTVRSTYKAGGGVPHLIAVYQDKSGAAR
                     DIAMSYAVANGGGKAGIIETSFREETETDLFGEQAVLCGGAVELIKAGFETLVDAGYA
                     PEMAYFECLHELKLIVDLIYEGGIGNMNYSISNNAEYGEYVTGPRVVTEATKAAMRQC
                     LTDIQTGEYAKSFILENRAGAPTLTSRRRLTAEHQIEVVGEKLRAMMPWIKANRLVDT
                     SKN"
     gene            complement(1549..2040)
                     /gene="ilvN"
                     /locus_tag="PK144_11720"
     CDS             complement(1549..2040)
                     /gene="ilvN"
                     /locus_tag="PK144_11720"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNF66443.1"
                     /translation="MRHIISILLENESGALSRVAGLFSARGYNIESLTVAPTEDESLS
                     RLTLVTYGNDAIIEQIVKQLNKLIDVVKLVDLTEGAHIERELMLVKVRATGELRAEMF
                     RLTEIFRGRMLDVSESTYTIEMTGPSDKLDAFLGTVGADNVVEVARSGVTGIARGPKG
                     LHV"
     gene            complement(2043..3785)
                     /locus_tag="PK144_11725"
     CDS             complement(2043..3785)
                     /locus_tag="PK144_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018122290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HNF66444.1"
                     /translation="MELTGAEIVVRCLKEEGVEYLFGYPGGSVLHIYDALHGQDDVKH
                     VLVRHEQAAAHAADGYSRASGKPGVVLVTSGPGLTNAVTGIATAYMDSVPMVILSGQV
                     PTKLIGNDAFQEVDSVGITRPCVKHNFLVKDVKDLAVTIKKAFYIATTGRPGPVLVDL
                     PKDITAARVEFDYPKKIKLRSYNPTVKGHAGQIRKAVELLVQARRPMIYTGGGVVLSD
                     GSKDLVELTRLLNFPITNTLMGLGAYPATDRQFVGMLGMHGTYEANLAMHHCDVLFAV
                     GARFDDRVTGNLEKFCPNARIIHIDIDPSSISKNVPVELPIVGSVPNVLRQMIAVLKE
                     EGLKPDAEAIAQWWRQIDEWRAIESLAYRNSDTIIKPQYVVEQLYKVTGGQAIVTSDV
                     GQHQMWAAQYYLFDRPRQWINSGGLGTMGFGLPAAMGAKFAFPDLDVACITGEGSIQM
                     MLQELSTMKQYGTPVKIVNLNNRYLGMVRQWQEFFYEKRYSMSYMEALPDFRKLAEAY
                     GHIGLQIEKPADVEGALREAFAMKDRTVFLDFLTDPEENVYPMIPAGGGQAEMLLAGR
                     DAMKNVHEEGMILV"
     gene            4061..4489
                     /locus_tag="PK144_11730"
     CDS             4061..4489
                     /locus_tag="PK144_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNF66445.1"
                     /translation="MNRARPALLLVLGWLALGALPADAAVYKWVDAEGRTHYSSTPPP
                     GADAKPVVIREDPLPPPAAGSPPAAPPAAGGGNADNAPRPPDQARAAACDTARHNLAL
                     LEKDGPVAKAGPDGKNVLLEGEARARELDRARKAVAYLCD"
     gene            4583..6295
                     /locus_tag="PK144_11735"
     CDS             4583..6295
                     /locus_tag="PK144_11735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNF66446.1"
                     /translation="MRVSRFPLYTVKETPADAEVISHKLMLRAGLIRKIAAGLYTWMP
                     LGLRVLRKVEAVVREEMNGIGCLEILMPAVQPAELWQESGRWQQYGPELLRLKDRHER
                     DFCFGPTHEEIITDLFRREIRSYRQLPVAFYQVQTKFRDEVRPRFGVMRAREFLMKDA
                     YSFHVDQASLQQTYDDMHGAYSRVFTRLGLDFRAVLADTGSIGGSGSHEFHVLADSGE
                     DDIAFSDASGYAANVELAEALAPAGERPAAGEALTRVHTPKVKTIAELAAFLKIPAER
                     TLKAVVVDGSDGRPVLLLLRGDHELNEIKAGKLPEVAEPLTFSTPAAISAAFGAQPGS
                     LGPVGFDGVVIADRSAAVLADAVTGANEDDWHLSGFNFGRDCPEPRVADLRNVVAGDP
                     SPDGQGRLSIRRGIEVGHIFQLGRKYSDALAANVLGEDGQNLTVTMGCYGIGVSRVVA
                     AAIEQNWDERGILWPAAMAPFQLALLPMNAHKSAAVREAVEQLYADCRAAGIDVLLDD
                     REARPGVMFADMELIGIPHRVVIGEKGLAAGSVEYKGRRDAEALAVPLAGLIDFLRER
                     LGAA"
     gene            6413..6907
                     /gene="nudB"
                     /locus_tag="PK144_11740"
     CDS             6413..6907
                     /gene="nudB"
                     /locus_tag="PK144_11740"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNF66447.1"
                     /translation="MAASRVFPDTRNFCRCPMTASPWKRPESVLVVVHTRGGLVLLLR
                     RLAPPDHWQSVTGSLEWHESAPKVAARRELLEETGIDGGRLIDTGVVNRYPILPPWRA
                     RYAPEATENREYVFLLELAEPCPIRLCPEEHDAVVWLPRLAAAQQVFSYTNREAVLAF
                     VPRQ"
     gene            6964..7542
                     /locus_tag="PK144_11745"
     CDS             6964..7542
                     /locus_tag="PK144_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNF66448.1"
                     /translation="MNQHSSRSRLRSAIALALCLGVGGLTLAGCASTATSESAGEYVD
                     NAAITAKVKEKYLADPGIKSLKIEVATYKGVVQLTGFVESADQKARAEQLAASVQGVE
                     SVKNEIVVLRKGETAGQYMDSAAITAKVKAKIAEDPHLSAFAIEVETQRGIVQLSGFV
                     DSSAQRTAAGRIARSVEGVKSVKNSLTVKPRS"
     gene            complement(7649..>7892)
                     /locus_tag="PK144_11750"
     CDS             complement(7649..>7892)
                     /locus_tag="PK144_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66449.1"
                     /translation="PAEGEASATAPTPAREPRRERRPRPDGEAPRRRADRGDRKEPRR
                     EREPVRASAAPEQPVESAFASLLREALAKQDAPEKP"
BASE COUNT         1217 a         2734 c         2675 g         1266 t
ORIGIN      
        1 ggttctcgac cttgcgccgc tgcgcgtaca gcggcgaatt ggcgatcttc agcagcttcg
       61 cggtcagggc cgggtcacgg cagaccacgt cggcgatctc gccgatgccg gcatccgggt
      121 cgtcgccgag ttcgatgatg cgcatcgcga cggcgggcag cgtcggcagg tgcgtgcagg
      181 caccgagtcg ggctagcagg gtttcatcca tcggcgtacg gcgtcctcga ctttcataag
      241 gatgcgacac gagcggcagg cggcgtgtcc ggaccgagcc taccataacc gccgcgtgac
      301 cctcgggtca tcccaggcga accggtcatg cctgcaggcg tacacgaaag cagacgacca
      361 gaccgcggtc gcacgcacga agcacgcata aaaaagggag ggctggcgcc ctcccttccg
      421 ctgtcgagcc ggcgcgctgc gccgggacgc gcttcttcag ttcttgctgg tgtcgaccag
      481 gcggttggcc ttgatccacg gcatcatcgc gcgcagcttc tcgccgacca cctcgatctg
      541 gtgctcggcg gtcagacggc ggcgcgaggt cagcgtcggg gcgccggcgc ggttttcgag
      601 gatgaagctc ttcgcgtact cgccggtctg gatgtcggtc aggcactggc gcatcgcggc
      661 cttggtcgcc tcggtcacga cgcgcgggcc ggtcacgtac tcgccgtact cggcgttgtt
      721 cgagatcgag tagttcatgt tgccgatgcc gccctcgtag atcaggtcga cgatcagctt
      781 cagctcgtgc aggcactcga agtaggccat ctccggcgcg tagccggcat cgaccagcgt
      841 ctcgaagccg gccttgatca gctcgacggc gccgccgcag agcacggcct gctcgccgaa
      901 cagatccgtc tcggtctcct cgcggaagct ggtctcgatg atgccggcct tgccgccgcc
      961 gttggcgacc gcgtaggaca tcgcgatgtc gcgggccgcg ccggacttgt cctgatagac
     1021 ggcgatcagg tgcggcacgc cgccgccggc cttgtaggtc gagcgcacgg tgtggcccgg
     1081 ggccttcggc gcgatcatga tcacgtcgag atcggcgcgc ggcacaacct ggttgtaatg
     1141 gatgttgaag ccgtgcgcga aggcgagcgc cgcaccctgc ttgatcttct tctcgatctg
     1201 ctgatagacg ccggcgatgt tctcgtccgg cagcaggatc atgaccacgt cggcgccctc
     1261 gacggcggcg tcgacttcct tgacgctcag gccggcgttg acggccttct gccacgaggc
     1321 accgccggca cgcaggccga cgacgacctg cacgccggac tcgctgaggt tgttggcgtg
     1381 ggcgtgaccc tgcgagccgt agccgatgat ggcaacggtc ttgccctgga tgagggagag
     1441 gtcggcgtct ttgtcgtagt aaacgttcat gcgggagttc cctgcgggcg gtagcgcggg
     1501 gcaggccttg gtagcggccg gccccgccag gaaggtggag tcagtcgttc agacgtgcag
     1561 gcccttcggc ccgcgcgcga tgccggtgac gccggaacgc gccacctcga cgacgttgtc
     1621 ggcaccgacg gtgccgagga aggcatcgag cttgtcgctc ggaccggtca tctcgatcgt
     1681 gtacgtgctc tcggacacgt cgagcatgcg gccgcggaag atctcggtga gccggaacat
     1741 ctcggcgcgc agctcgccgg tcgcgcgcac cttcacgagc atcagctcgc gctcgatgtg
     1801 cgccccttcg gtcaggtcga cgagcttgac cacgtcgatc agcttgttca gctgcttgac
     1861 gatctgctcg atgatcgcgt cgttgccgta ggtgaccagc gtcagccgcg acagcgactc
     1921 atcctcggtc ggcgcgacgg tcagcgactc gatgttgtag ccgcgggcgg aaaacaggcc
     1981 cgccacgcgc gacagggcgc cggattcgtt ctccagcagg atcgaaatga tgtgtcgcat
     2041 gattacacca ggatcatccc ttcttcgtgc acgttcttca tcgcgtcgcg cccggcgagc
     2101 agcatctcgg cctggccgcc gccggcaggg atcatcgggt agacgttctc ttcgggatcg
     2161 gtcaggaagt cgaggaacac ggtgcggtcc ttcatcgcga aggcttcgcg cagtgcgcct
     2221 tcgacatcgg ccggcttctc gatctgcagg ccgatgtggc cgtaggcctc ggcgagcttg
     2281 cggaagtccg gcagcgcttc catgtacgac atcgagtagc gcttctcgta gaagaactcc
     2341 tgccactggc gcaccatgcc cagatagcgg ttgttcaggt tcacgatctt caccggcgtg
     2401 ccgtactgct tcatcgtcga gagttcctgc agcatcatct ggatgctgcc ctcgccggtg
     2461 atgcaggcca cgtcgaggtc cgggaacgcg aacttcgcgc ccatcgccgc cggcaggccg
     2521 aagcccatcg tgccgaggcc gccggagttg atccactggc gcggacggtc gaacaggtag
     2581 tactgcgcag cccacatctg gtgctggccg acgtcggagg tcacgatcgc ctggccgccg
     2641 gtgaccttgt agagctgctc gaccacgtac tgcggcttga tgatcgtgtc gctgttgcgg
     2701 tacgcgagcg actcgatcgc gcgccattcg tcgatctgcc gccaccactg ggcgatggcc
     2761 tcggcatcgg gcttgaggcc ttcctccttc agcaccgcga tcatctggcg cagcacgttc
     2821 ggcaccgagc cgacgatcgg cagctcgacc ggcacgttct tcgagatcga cgacggatcg
     2881 atgtcgatgt ggatgatgcg ggcgttcggg cagaactttt cgaggttgcc ggtcacgcgg
     2941 tcatcgaagc gcgcaccgac ggcgaacagc acgtcgcagt gatgcatcgc cagattcgct
     3001 tcataggtgc cgtgcatgcc gagcatgccg acgaactggc ggtcggtggc cggataggca
     3061 ccgagcccca tcagcgtgtt ggtgatcggg aagttcagca gccgggtgag ctcgacgaga
     3121 tccttcgaac cgtccgacag cacgacgccg ccgccggtgt agatcatcgg ccggcgcgcc
     3181 tgcaccagca gctcgaccgc cttgcggatc tggccggcgt ggcccttcac ggtcgggttg
     3241 tacgagcgca gcttgatctt cttcgggtag tcgaactcga cgcgcgcggc ggtgatgtcc
     3301 ttcggcaggt cgaccagcac cggaccgggg cggccggtcg tggcgatgta gaaagccttc
     3361 ttgatcgtga ccgcgagatc cttcacgtcc ttgaccagga agttgtgctt cacgcacgga
     3421 cgcgtgatgc cgaccgagtc gacctcctgg aaggcatcgt tgccgatcag cttggtcggc
     3481 acctgcccgg acaggatcac catcggcacc gaatccatgt aggcggtggc gatgccggtg
     3541 acggcgttgg tcaggcccgg accggaggtc acgagcacga cgccgggctt gcccgaggca
     3601 cgcgagtaac catcggcggc gtgcgcggcg gcctgttcgt gccgcaccag cacgtgcttc
     3661 acgtcgtcct ggccgtgcag cgcatcgtag atgtgcagta cggagccgcc cggatagccg
     3721 aagaggtatt cgaccccctc ttccttcagg caacggacaa cgatctcggc accggtgagt
     3781 tccacggcag gagcctcctg ggactgggca tatgggttga cggcggcgtg gcgccgcgca
     3841 gactggcaaa atcgccggta tcgtcgtggc tgcgcgcccc gcggatcagc aggaacggcc
     3901 tttcgggcac cgcccgcgga gtcgcggacg gggtcatcgg tggcagccag ttcgcacgga
     3961 ccgggcaaac tatccgcagc ccccccaaag gtcaaggacg gcgggcattc ccgccgcacg
     4021 accgccctcc caacacgctg aaaccgcagg agtacgcatc atgaaccgcg cccgcccggc
     4081 cctgctgctg gttctcggct ggctcgcact cggcgccctg cccgccgatg ccgcggtcta
     4141 caagtgggtc gatgccgaag gccgcacgca ttattcgtcc acgcctccgc ccggggccga
     4201 cgcgaagccg gtcgtcatcc gcgaggaccc gctgccgccg cccgccgccg gttcgccgcc
     4261 ggccgccccg ccggctgcgg gcgggggcaa tgccgacaac gcgccgcgcc cgcccgatca
     4321 ggcgcgcgcc gccgcctgcg acaccgcccg ccacaacctc gcgctgctcg aaaaggacgg
     4381 ccccgtcgcc aaggccggcc cggacggaaa gaacgtgctg ctcgaaggcg aggcgcgcgc
     4441 ccgcgagctg gatcgcgcgc gcaaggccgt cgcgtatctc tgcgactgag gccgccggcg
     4501 ccgccccctg cgccggcgca gtgcggctcc ggtacactcg cgccctttcc gtctcgcgag
     4561 tcaccggagt cccgccgccg ccatgcgcgt atcccgcttc ccgctctaca ccgtcaagga
     4621 aaccccggcc gacgccgagg tgatcagcca caagctgatg ctgcgcgccg gcctgatccg
     4681 caagatcgcc gccggcctct acacgtggat gccgctcggg ctgcgcgtgc tgcgcaaggt
     4741 ggaggcggtc gtgcgcgagg agatgaacgg catcggctgc ctggagatcc tgatgccggc
     4801 cgtgcagccg gccgagctct ggcaggaatc cggccgctgg cagcagtacg gccccgagct
     4861 gctgcgcctg aaggaccgcc acgagcgcga cttctgcttc ggcccgacgc acgaggagat
     4921 catcaccgat ctcttccggc gcgagatcag gagctaccgg cagctgccgg tcgcgttcta
     4981 tcaggtgcag acgaagttcc gcgacgaggt gcggccgcgc ttcggcgtca tgcgcgcgcg
     5041 cgagttcctg atgaaggacg cctactcctt ccacgtcgat caggcctcgc tgcagcagac
     5101 ctacgacgac atgcacggcg cctactcgcg cgtgttcacg cgcctcggcc tcgacttccg
     5161 ggccgtgctc gccgacaccg gctcgatcgg cggctccggc tcgcacgaat tccacgtgct
     5221 cgccgactcc ggcgaggacg acatcgcctt ctccgatgcc tccggctacg ccgccaacgt
     5281 cgagctggcc gaagccctgg ccccggccgg cgaacgcccg gccgccggcg aggcgctgac
     5341 gcgcgtgcac acgccgaagg tcaagaccat cgccgagctt gccgccttcc tgaagatacc
     5401 ggccgaacgc acgctgaagg ccgtcgtcgt cgacggcagc gacggccggc cggtgctgct
     5461 gctgctgcgc ggtgaccacg agctgaacga gatcaaggcc ggcaagctgc cggaagtggc
     5521 cgagccgctg acgttctcga ccccggctgc gatcagcgcc gccttcggcg cccagcccgg
     5581 ctcgctcggc ccggtcggct tcgacggcgt ggtcatcgct gatcgcagcg ctgccgtgct
     5641 cgccgatgcc gtgaccggcg ccaacgagga cgactggcac ctcagcggct tcaacttcgg
     5701 ccgcgactgc ccggagccgc gcgtcgccga cctgcgcaac gtcgtcgccg gcgacccgag
     5761 cccggacggc cagggccggc tttcgatccg ccgcggcatc gaggtcggcc acatcttcca
     5821 gctcgggcgc aagtacagcg acgcgctcgc cgcgaacgtg ctcggcgagg acggccagaa
     5881 cctgaccgtg accatgggct gctacggcat cggcgtctcg cgcgtcgtcg ccgccgcgat
     5941 cgaacagaac tgggacgagc gcggcatcct gtggccggcg gcgatggcgc cgttccagct
     6001 cgcgctgctg ccgatgaacg cgcacaagag cgccgccgtg cgcgaggcgg tcgagcagct
     6061 ctacgccgac tgccgggccg ccggcatcga cgtgctgctc gatgaccgcg aggcgcgccc
     6121 cggcgtgatg ttcgcggaca tggagctgat cggcatcccg caccgcgtcg tcatcggcga
     6181 gaaggggctt gccgccggca gcgtcgagta caagggccgg cgcgacgccg aagccctcgc
     6241 cgtgccgctt gcaggtctca tcgacttcct gcgcgagcgc ctcggcgcgg cctgagggaa
     6301 gacccttccc gggccaggca gctgcgcgca ggacgcgcgc ctgccgggtg cgcaggcccg
     6361 gcgccccgag cgggcgcgcg tcaggctcat cgacgcctcc ggagcggccg caatggccgc
     6421 ttcccgggtc ttcccggata cccgcaactt ttgcaggtgc ccgatgaccg cctcgccatg
     6481 gaaacgcccc gaatccgtgc tcgtcgtcgt gcacacgcgc ggcggcctcg tgctgctgct
     6541 gcgccgcctc gccccgcccg atcactggca gtcggtgacc ggcagcctcg aatggcacga
     6601 gtccgcccca aaagttgcgg cccggcgcga actcctggag gaaaccggga tcgatggggg
     6661 caggctgatc gataccggcg tcgtcaaccg ctatccgatc ctgccgccgt ggcgggcgcg
     6721 ctacgcgccc gaagccacgg aaaaccggga atacgtgttt ctgctcgaac tcgcggaacc
     6781 ttgtccgatc cggttgtgtc ctgaggaaca cgacgctgtc gtctggctgc cacgtttggc
     6841 agcagcgcaa caggtgtttt cgtatacgaa tcgcgaggcc gtgctcgcgt tcgtccccag
     6901 gcagtgaaac cgctgtccag ctgcactccg gcagccattc agaacaacag aaggagaatc
     6961 ccgatgaacc agcactcgag ccgttcgcgc ctgcgttccg ccattgcact ggccctctgc
     7021 ctcggcgtcg gtggcctgac cctcgccggt tgtgccagca cggccaccag cgagagcgcc
     7081 ggcgagtacg tcgacaacgc cgcgatcacc gccaaggtca aggagaagta cctggccgat
     7141 ccgggcatca agagcctgaa gatcgaggtt gcgacctaca agggcgtggt gcagctgacc
     7201 ggcttcgtcg agtcggccga ccagaaggcg cgcgccgagc agctcgccgc gagcgtccag
     7261 ggcgtcgagt cggtcaagaa cgagatcgtc gtgctgagaa agggcgagac cgccggtcag
     7321 tacatggaca gcgccgcgat caccgccaag gtcaaggcga agatcgccga ggacccgcac
     7381 ctcagcgcct tcgcgatcga ggtcgagacg cagcgcggca tcgtgcagtt gagcggcttc
     7441 gtcgacagct ccgcgcagcg cacggcggcc ggtcgcatcg cgcgctcggt cgagggcgtg
     7501 aagtcggtga agaacagcct gaccgtcaag ccgcgcagct gagcggctgc cgcgtcgccc
     7561 gtcatcgggg cccggccgga gcgatccggc cgggccccgg cgtttttcag cgcggctccc
     7621 tgccgacatg gcgggagccg ttgccgcctc agggcttttc cggggcgtcc tgcttggcca
     7681 gcgcctcgcg cagcagcgac gcgaacgcgc tctcgaccgg ctgctcgggc gcggcgctgg
     7741 cgcgcaccgg ttcgcgctcg cggcgcggtt ccttgcgatc accgcgatcg gcacggcggc
     7801 gcggcgcctc gccatccgga cgcggccggc gctcgcggcg cggctcgcgg gcgggggtgg
     7861 gggcggtggc agaagcctcg ccttcggccg gc
//
LOCUS       DAOQIE010000182         7873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4781_length_7873_cov_7.171655, whole genome shotgun sequence.
ACCESSION   DAOQIE010000182 DAOQIE010000000
VERSION     DAOQIE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4781_length_7873_cov_7.171655"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..231)
                     /locus_tag="PK144_11755"
     CDS             complement(52..231)
                     /locus_tag="PK144_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66450.1"
                     /translation="MNHTIPENLTVAVETTARLLGEIRRQRNLRQQMSDPSMRTNAAT
                     RQPPVPHHGSPGDTH"
     gene            533..1774
                     /locus_tag="PK144_11760"
     CDS             533..1774
                     /locus_tag="PK144_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF66451.1"
                     /translation="MNSLFWSGRPFVLAATLACALLSACSVPARLPAVPAEQTTQATV
                     PGMVNVRYWPDVDTAALERLSLETIRRMQAYRAAQGQTGPLPPAHFLALSGGGDDGAF
                     GAGLLNGWTATGTRPSFGIVTGISTGALIAPFAFLGPAYDPVLRKVYTETRQADIFLT
                     RPVFAAINNDAMADTQPLWTLIGQNVDEDFLARIGAEYRKGRLLLIATTDIDARRPVL
                     WDMGAIATSGAPGALDLFRRIMLASAAIPGVFPPVLIDVEVDGKSHQEMHVDGGAAAQ
                     VFLYPPSLNVKVLSKRYGIERERHAWIIRNARLDPSWASVERRTMSIAGRAVSSLIQY
                     QGVGDLYRIYATTQRDGVDYNLAYIGSDFRVKHRDDFDPEYMRPLYEYGERLARNGYP
                     WQKTPPGYIAPPPAKTMVVQH"
     gene            2061..3470
                     /locus_tag="PK144_11765"
     CDS             2061..3470
                     /locus_tag="PK144_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF66452.1"
                     /translation="MPIPRDETRAARARADGLDLKRLLLRRVTLFALAICLGGIVTVL
                     VQTQAELRRQVARIGPTLLERLINEEAMQPRGSFLMGLEELKLASLDPLGEWLDICVE
                     AEDLFGRQRVRRCFGERLALPAALRGLPALLLDGRELYRGPIVQYPGYRAGEFLITPN
                     LDSLARSAWRQIRTVLGMTAGILLLNLFVYLPVRRALRPSEQILAVMEKMRAGDLSVR
                     MPRPALHELQRIASGFDELADRLQQTLDEQRRLAQRLIAVREEERRHLGRELHDEFGQ
                     CLTSIGAEAVFIGGQVRERLPELLPAVGSISEVTAKMLEALQGILSQLRPVGLESFGL
                     QAILEQFLAGWQRRQPDCRFTLDLDGPLDDLPDELTVSLYRIVQESLTNALRHGSPTQ
                     VTVQLQRHDRHCRLCIEDDGEAGENPTAGSGLGVLGMRERVLALGGRFSMTPRSPRGM
                     RVLAEFPAESLSAERSFHA"
     gene            3463..4116
                     /locus_tag="PK144_11770"
     CDS             3463..4116
                     /locus_tag="PK144_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF66453.1"
                     /translation="MPDPIRVLLIDDHVVVRVGYRRLLEAQPDIRVAGEAATAREALD
                     WLRQQEAATTEPELVILDLGLPDLGGVELIRRLLQRQPRLRILVFTMHREPIFATQAL
                     RAGALGYVTKSSPPEVLIDAVYRVAARRQTISPDIAPDLALALIAPPEKPLAESLSPR
                     EFEILRLLLEGSSPEEIGARLSISAKTVQNCHYQIKTKLGARSDIELTRIALRAGLI"
     gene            complement(4227..4895)
                     /locus_tag="PK144_11775"
     CDS             complement(4227..4895)
                     /locus_tag="PK144_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66454.1"
                     /translation="MSPSDGHDSPAAAGHRRPAGQTGSPRAGVPRPGRSRSPPRAERP
                     PDRRQSAVAAVAGRALQWRWHQPDAGLCRRIPMPEHTFDDSSIRWNRFGEFEPAFHFT
                     ILDIDESRRITDVLFRLPAEAQIVLHRHLVLNHTFVVQGEHRLYEPDGSLREARATGT
                     HTVSPASERPHREGGGAQDAIVHFSIRPDGADDLYELLDDAGNPLARVTWPMLVELYR
                     AQAA"
     gene            4964..6418
                     /locus_tag="PK144_11780"
     CDS             4964..6418
                     /locus_tag="PK144_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66455.1"
                     /translation="MKVSRAELIAVLAQFNPWWRGEGIGDLPRWRRAAFRELSRWLLA
                     PPAPRAVLLSGARQIGKTTLLLQAVEVLLQAGVPAANIVYATFDHPLLKLAGMEAVLE
                     AWREREPRTDGPEYLLLDEAQFIRDWGTWIKHQVDFRKDRRIVFTGSAMPLTEAGQES
                     GVGRWHTIRLTTLSFYEYLQIKRLTLPELPPLRSLRELFDWPPAQFYRATEAASAYAG
                     HFHEFLLRGGFPQSAQVDSITQAQRLLREDIIDKVLKRDMTALFGVRRVLDLEHTFLY
                     LCLHDGGLLDMGDLCSNLEVKRPTAQSFIELLEATHLIYRLPPFGYGKDVLRGRFKIY
                     LADAAIAPAVMLKGKNLLDDAAALGVATETAVFKHLFARYYARNVRFSYWRGRRDHEV
                     DLVAEADGELIPFEVKYRARHTGARELKGLIELCGQKQITRGYVVTKALDDFGLLSGL
                     PAAPDGQAADIRIMRIPAALLCYWMGASELVAED"
     gene            6860..7315
                     /locus_tag="PK144_11785"
     CDS             6860..7315
                     /locus_tag="PK144_11785"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNF66456.1"
                     /translation="MDSPDQEPGRADDEGPQHTVTVPAFALNRTEVSFADYDRFAETT
                     GRRKPADRWGHGNQPVINVSWNDAQAYAVWLSEQTGRKYRLPSEAEWEYAARAGTTTP
                     FWTGNCIRTDQANYDGTFDYNNFCDSVLRMPDLPVWRSDRWRHAPERGR"
BASE COUNT         1339 a         2668 c         2597 g         1269 t
ORIGIN      
        1 cgcagccgga atcagccgga atcagccgga atcagccgga atcagccgga atcagtgcgt
       61 gtcgccgggg ctgccgtggt ggggcaccgg cggctggcgc gtggcggcgt tcgtgcgcat
      121 ggacggatcg ctcatctgct ggcgcaggtt gcgctggcgg cggatctcgc cgagcaggcg
      181 ggcggtggtt tcgacggcga cggtcaggtt ttcgggaatg gtgtggttca tggcgtctgt
      241 ccttggcgtt cgaaggcggc taaacagctg attggctgtc agaaacactg tacaacgaca
      301 gcattgctta ccactgcgac ttctctcaat acgatatctg tcgtattcgt aatgtacgaa
      361 agaagttgca acaccgttcg tctgcggact gaggcaaggc tgcgggcgag tccgcggggc
      421 tgcaccgccg gcacggacac gcaagccatg cgcggccccg ggcacgcgcg cgagacatgt
      481 ctacgctcgg catgccaccg taggcgctgg tatcgcatcg aggacaaccg ccttgaacag
      541 tctcttctgg tccggccggc cgttcgtgct ggctgccacc ctggcatgcg cgctgctttc
      601 ggcctgttcg gtgccggcac ggctgcctgc cgtgcccgcc gagcagacca cgcaggcaac
      661 cgtgcccggc atggtcaacg tccgttactg gccggatgtc gacaccgccg ccctggagcg
      721 gctcagcctc gaaaccatcc ggcgcatgca ggcataccgc gcggcccagg gccagaccgg
      781 accgctgccg ccggcgcatt tcctcgcgct gtcggggggc ggcgatgacg gtgccttcgg
      841 ggccgggctg ctgaacggct ggacggccac cggcacgcgc ccgagcttcg gcatcgtcac
      901 cggcatcagc accggggcgc tgatcgcgcc gtttgcgttc ctcggcccgg cctacgaccc
      961 ggtgctgagg aaggtctaca ccgagacccg gcaggcggac atcttcctga cgcggccggt
     1021 gttcgcggcg atcaacaacg atgcgatggc cgacacgcag ccgctctgga cgctgatcgg
     1081 ccagaacgtc gacgaggact tcctcgcacg catcggcgcc gaataccgca agggccggct
     1141 gctgctgatc gcgaccaccg acatcgacgc ccgccggccg gtgctgtggg acatgggcgc
     1201 gatcgccacg agcggtgcgc ccggcgcgct cgatctgttc cggcgcatca tgctggcctc
     1261 ggcggcgatc ccgggcgtgt tcccgccggt gctgatcgat gtcgaggtcg acggcaagtc
     1321 ccatcaggag atgcacgtcg acggcggtgc cgccgcgcag gtgttcctgt atccgccgtc
     1381 gctgaacgtg aaggtgctgt cgaagcgcta cggcatcgag cgcgagcgcc atgcgtggat
     1441 catccgcaac gcccggctcg atccgtcctg ggccagcgtc gagcgccgca cgatgagcat
     1501 cgccggccgg gcggtgtcgt cgctgatcca gtaccagggc gtcggcgatc tgtaccgcat
     1561 ctacgcgacc acgcagcgcg acggggtcga ctacaacctc gcctatatcg gcagcgactt
     1621 ccgggtcaag caccgcgacg acttcgaccc cgagtacatg cgtccgctgt acgaatacgg
     1681 cgaacgcctc gcccgcaacg gctatccgtg gcagaagacg ccgccgggct acatcgcacc
     1741 gccgccggca aagacgatgg tggtccagca ctgacaggcg cgggcaatcc gcgaccggcg
     1801 caccggctgc cagcctcgcc agcaggccat tgccgtccgg ccggacgtgc cggacagcac
     1861 ccgccgcatc ggctcgaccg ccccttcccg cccgccccgg acctgaaagc cccggggcgg
     1921 cgaggcatcc cggacctcaa aaaagccctg cccctcgcga ttgcgggccg gcaagcccgc
     1981 gtccgtcccc tgccctttcc ccgcgtccgg actgcttcat gatttcccgg aacccgacag
     2041 cccggagaca ggtggccacg atgccgatcc cccgtgacga aacccgcgct gcacgcgcac
     2101 gggcagacgg gctcgacctc aagcgcctgc tgctgcggcg cgtgacgctg ttcgcgctgg
     2161 cgatctgcct cggcggcatc gtcacggtgc tggtgcagac gcaggccgaa ctgcgccggc
     2221 aggtcgcgcg catcgggccg acgctgctgg agcggctgat caacgaggaa gccatgcagc
     2281 cgcgcggcag cttcctgatg gggctggagg agctgaaact cgcgtcgctg gacccgctcg
     2341 gcgaatggct ggacatctgc gtcgaggccg aggacctctt cggccgccag cgcgtgcgcc
     2401 gctgcttcgg cgaacgcctg gcgctgccgg cggcgctgcg cggcctgccg gcgctgctgc
     2461 tcgatggccg cgagctgtac cgcggtccga tcgtccagta ccccggctac cgggccgggg
     2521 aattcctcat cacgccgaac ctcgacagcc tggcgcgctc ggcctggcgg cagatccgca
     2581 ccgtgctcgg catgaccgcc ggcatcctgc tgctgaacct gttcgtgtat ctgccggtgc
     2641 gccgggcgct gcgcccgagc gagcagattc tggcggtcat ggagaagatg cgcgccggcg
     2701 acctgtcggt gcgcatgccg cgtccggccc tgcacgagct gcagcgcatc gccagcggct
     2761 tcgacgagct cgccgaccgg ctgcagcaga cgctcgacga acagcgccgg ctggcgcagc
     2821 ggctgatcgc cgtgcgcgag gaggaacgcc gccacctcgg ccgggagctg cacgacgaat
     2881 tcggccagtg cctgacctcg atcggcgccg aggcggtgtt catcggcggt caggtgcgcg
     2941 agcgcctgcc cgagctgctg ccggcggtcg gctcgatcag cgaggtgacg gcgaagatgc
     3001 tggaggcgct gcagggcatc ctgagccagt tgcgcccggt cgggctggaa tccttcggcc
     3061 tgcaggcgat cctcgaacag ttcctggccg gctggcagcg ccgccagccg gactgccgct
     3121 tcacgctcga tctcgacggc ccgctcgacg acctgcccga tgaactcacc gtcagcctct
     3181 accgcatcgt gcaggagagc ctgaccaatg ccctgcgcca cggctcgccc acgcaggtga
     3241 cggtgcagct gcagcgccat gaccggcact gccggctctg catcgaggac gacggcgagg
     3301 ccggcgaaaa tccgacggcc ggcagcggac tcggcgtgct cggcatgcgc gagcgcgtcc
     3361 tcgcgctggg cgggcggttc tcgatgacgc cgcgcagccc gcgcggcatg cgcgtgctgg
     3421 ccgaattccc ggccgaatcc ctcagtgccg agcggagctt ccatgcctga tcccatccgg
     3481 gtgttgctga tcgacgatca cgtcgtggtc cgcgtcggct accggcgcct gctggaggcg
     3541 cagcccgaca tccgcgtcgc cggcgaagcc gcgaccgcgc gcgaggcgct ggactggctg
     3601 cggcagcagg aggccgcgac cacggaaccc gaactggtga tcctcgacct cggcctgccc
     3661 gatctcggcg gcgtcgaact gatccgccgg ctgctgcagc gccagccgcg cctgcgcatc
     3721 ctggtgttca cgatgcaccg cgagccgatc ttcgcgacgc aggcgctgcg cgccggcgcc
     3781 ctcggctacg tgaccaagag cagcccgccg gaagtgctga tcgacgccgt gtaccgggtc
     3841 gccgcgcgcc gccagacgat cagcccggac atcgccccgg acctggcgct ggccctgatc
     3901 gcgccgccgg aaaaaccgct cgcggaatca ctgtcgccgc gcgaattcga aatcctgcgc
     3961 ctgctgctgg aaggcagcag ccccgaggaa atcggcgcgc gactgtcgat cagcgccaag
     4021 accgtgcaga actgccatta ccagatcaag accaaactcg gcgcccgcag cgacatcgaa
     4081 ctgacgcgca tcgccctgcg tgccgggctg atctgatgcc ggatcatgcc gatcacaaat
     4141 gaacacgccg tcttttcagc ccctcgcccc tcgcgggaga ggggctgggg agaggggaaa
     4201 aaccgcgcat ttacgtgtga tcggtattac gccgcctgcg cccggtacaa ctcgaccagc
     4261 atcggccagg taacccgcgc gagcggattg ccggcatcgt cgagcagttc atacaggtca
     4321 tcggcaccat cggggcggat gctgaaatgc acgatggcat cctgcgcgcc gccgccttcg
     4381 cgatgcggcc gctcgctggc cggcgaaacg gtatgcgtgc cggtggcgcg cgcttcgcgc
     4441 aggctgccat ccggctcata cagccgatgc tcgccctgca cgacgaaggt gtgattcagc
     4501 accagatgcc ggtgcagcac gatctgcgct tcggcgggca ggcggaacag cacatcggtg
     4561 atacggcggc tttcgtcgat atccagaatc gtgaaatgaa acgccggctc gaattcgccg
     4621 aaacggttcc agcggatcga tgaatcgtcg aaggtgtgct cgggcatcgg aattctcctg
     4681 cagaggccgg catcgggctg atgccaccgc cattgcagcg cccggccggc aacggcagcc
     4741 acagcagact gccggcgatc gggcgggcgt tctgcccgcg gaggggatcg ggatcggccg
     4801 gggcgaggga cgcctgcgcg cgggctgccg gtctgtcctg ccggccggcg gtggcccgcg
     4861 gctgccgggc tgtcgtgccc gtccgacggc gacatgagac caatcatcgc aatcagtgaa
     4921 ggtatacttt cactaaatcc aaaaaatagt gagatttcac gcgatgaagg tctccagagc
     4981 cgagctgatc gccgtgcttg cgcagttcaa tccgtggtgg cgcggcgagg gcatcggcga
     5041 tctgccccga tggcggcggg cagccttccg ggagctgtcc cggtggctgc tcgcgccgcc
     5101 ggcgccgcgt gcggtcctgc tgtccggcgc gcgccagatc ggcaagacca cgctgctgct
     5161 gcaggccgtg gaggtgctgc tgcaggcggg cgtgccggcg gccaacatcg tctatgcgac
     5221 cttcgaccat ccgctgctga agctcgccgg catggaggcc gtgctcgaag cctggcgcga
     5281 gcgcgagccg cggacggatg ggccggaata cctgctgctc gacgaagccc agttcatccg
     5341 cgactggggc acctggatca agcatcaggt cgacttccgc aaggaccggc gcatcgtgtt
     5401 caccggctcg gcgatgccgc tgacggaagc cggccaggaa tccggtgtcg gccgctggca
     5461 caccatccgg ctgaccacgc tgtcgttcta cgaatacctg cagatcaagc gcctgacgct
     5521 gccggagctg ccgcccctgc gcagcctgcg cgaactgttc gactggccgc cggcgcagtt
     5581 ctaccgcgcg accgaagccg ccagcgccta tgccggccat ttccacgaat tcctgctgcg
     5641 cggcggtttt ccgcagagcg cgcaggtcga cagcatcacg caggctcagc ggctgctgcg
     5701 cgaggacatc atcgacaagg tgctcaagcg cgacatgacg gcgctgttcg gcgtgcgtcg
     5761 cgtgctcgat ctggaacaca ccttcctgta cctgtgcctg cacgatggcg gtctgctcga
     5821 catgggcgac ctgtgctcga atctggaggt caagcgcccg accgcgcaga gtttcatcga
     5881 actgctcgaa gcaacgcacc tgatctatcg cctgccgccg ttcggttacg gcaaggacgt
     5941 gctgcgcggg cggttcaaga tttatctggc cgatgccgcg attgcaccgg ccgtgatgct
     6001 gaagggcaag aacctcctcg acgatgccgc cgctcttggg gtggcgaccg aaaccgcggt
     6061 gttcaagcac ctgttcgccc gctattacgc gagaaacgtg cgtttcagct actggcgcgg
     6121 gcgcagggat catgaagtcg atctggtggc cgaggccgac ggcgaactga tcccgttcga
     6181 ggtcaagtac cgcgcccggc acaccggcgc ccgcgaactc aagggcttga tcgaactgtg
     6241 cggacagaag cagatcacgc gcggctacgt cgtcaccaag gcactcgatg acttcggact
     6301 gctgagcggc ctgccggcag cccctgacgg gcaggcggca gacatccgga tcatgcggat
     6361 accggcggcg ctgctgtgtt actggatggg ggcgagtgaa ctggtggcag aagattgaga
     6421 cagggatgct ctgcatggaa ccatcacccg ctcgaacaac ccgatatccc agattggaaa
     6481 atcgattcag aacatactgc acaaatttgg aaatcgtctt actgacttag gaaataaaat
     6541 attcacaaaa atacgcatcg ctctggcgac agcattgatg accggtctga tcctgttgtt
     6601 ttctgggaaa cttccttacc agcacgattt ggctgcctct ctgtccaact ggcttaacaa
     6661 ccttgtcagt agtaacacaa ccccaagcaa tcctcctacg tttcgagatc ctttcaccca
     6721 taaattaact ccagcagaag tcgctgaaat caaaagctca ggcgctccat caccaacgac
     6781 actgaaacct tttcgcgccc cctgaagatc ggcggcttcg gccccgagat gattccgctg
     6841 ccggccggca ccgttcacga tggactcacc ggatcaggag ccggggcgcg ccgacgacga
     6901 aggcccgcag cacaccgtga ccgtaccggc tttcgcgctc aaccgcaccg aggtcagctt
     6961 tgccgattac gaccgctttg ccgaaacgac cggccgcagg aaaccggcag atcgctgggg
     7021 ccacggcaat cagccggtca tcaacgtgag ctggaacgat gcacaggctt acgccgtctg
     7081 gctcagcgag cagaccggca ggaaataccg cctgccgagc gaggcggaat gggaatatgc
     7141 ggcccgcgcc ggcacgacga cgccattctg gactggcaac tgcatcagaa ccgatcaggc
     7201 caattacgac ggaaccttcg actacaataa cttttgtgac tctgtgctgc gaatgccgga
     7261 cttgcctgtc tggagaagtg atcgatggcg gcatgcacct gaacgcggcc ggtgaaatga
     7321 ttcaggcggt ttagggcgaa attccggaaa attatccgga tgtcgggatt gccgggtttg
     7381 tcgtcatgcc caatcacgtt cacggcatca tcgtgctcgc aggggcaagc ccgcgtgtag
     7441 ggcccccccc gtggttgccc agattttagg tcgccaccaa tggcagcagg gcaaccaccg
     7501 ggggttgccc ctacggcgtg gggacgtatt gagggcgatg gcaccggttc ccggcagtat
     7561 ccgccgtagg ggcaggcccc cgtgcctgcc ccgaatcccg cgcctgcccg gaatccggcg
     7621 ccggtgccgg tcccggggca accaccgggg gttgccccta cggcgtggga acggatgcgg
     7681 acgatggcac cggttcccgg cggtatccgc ccgtaggggc aggcccccgt gcctgccccg
     7741 cccgtgcccg ccccgccccg gaatcccgtg cctgcccgga atccggcgcc ggtgccggtt
     7801 ccggggcaac caccgggagt tgcccctacg gcgtggggac gtattgaggg cgatggcacc
     7861 ggttcccggc agt
//
LOCUS       DAOQIE010000183         7814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4839_length_7814_cov_8.219100, whole genome shotgun sequence.
ACCESSION   DAOQIE010000183 DAOQIE010000000
VERSION     DAOQIE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4839_length_7814_cov_8.219100"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK144_11790"
     CDS             <1..196
                     /locus_tag="PK144_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66457.1"
                     /translation="SFACEEYVYSLPDIEDYIGQKIPVEPIEPGLLAEVRPARRSEHH
                     GERRPHGRPPRRRHNGGRPG"
     gene            complement(301..1041)
                     /locus_tag="PK144_11795"
     CDS             complement(301..1041)
                     /locus_tag="PK144_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007204108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF66458.1"
                     /translation="MPSDWKAEPAGLLPLGFEAACYAVGKRTLLGPIDCVLDSTPGVH
                     LVLGPNGAGKSLFLRLAHGLLQPTGGQVRWATEAAACRQRQAMVFQHPVVLRRPVRAN
                     VEYALGLRGLARAERHRRSEEALARAGLTALAARMAPVLSGGERQRLALARAWALHPD
                     VLFLDEPTASLDPAAAAQVEALIGDFRARGTHIVMSTHDLAQARRLGGEVLFFHRGRL
                     LERAPAAGFFAQPATREAAAYVRGELFW"
     gene            complement(1019..1723)
                     /locus_tag="PK144_11800"
     CDS             complement(1019..1723)
                     /locus_tag="PK144_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66459.1"
                     /translation="MNELVAAFWLAGDLLAGGDAQLYGIVVLSLQLSAGAVLVSTLLG
                     LPFGALLGLGRFPGRRVLIVITNAFMGMPPVVVGLFVYLMLSRSGPLGELGWLFRPEA
                     MVVAQVVLITPIVVALSRQIVEDLWAQLGDQLRSLGCSPLRAVPTLLREGRWSLLTVV
                     LAGFGRSVSEVGAIIIVGGNIAHVTRTMTTAIALETSKGDLPLALALGLILILIALLV
                     NVAADVVKTRAQRLEG"
     gene            complement(1746..2609)
                     /gene="purU"
                     /locus_tag="PK144_11805"
     CDS             complement(1746..2609)
                     /gene="purU"
                     /locus_tag="PK144_11805"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNF66460.1"
                     /translation="MPYAAAHFILTLACPDTTGIVAAVSGFLAEYGNNIVQSDQFGDA
                     DTGRFFLRVSFCASERLPDATAMRAAFTPIGERFAMDWEIHDTAIKPRMVILVSKFGH
                     CLNDLLYRYRVGALNVEIPAVISNHREFYQLAASYNIPFHYLPIMNDDKAAQESRILE
                     IIRHEQADLVVLARYMQVLSDAMCRELEGRAINIHHSFLPSFKGARPYHQAYERGVKR
                     IGATAHYVTADLDEGPIIEQEVAPVNHAMTPDDLVAVGRDLENVVLARAVKAHLERRV
                     LLNGRRTVVFP"
     gene            2806..3684
                     /gene="folD"
                     /locus_tag="PK144_11810"
     CDS             2806..3684
                     /gene="folD"
                     /locus_tag="PK144_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNF66461.1"
                     /translation="MADIIDGKAHAAGLRAHLARRVAQLHADAGFVPGLAVVLVGEDP
                     ASQVYVRNKAAQTRECGMQSIEHRLPASTTQDELLALVATLNADPEVDGILVQLPVPA
                     HIDPERVLLAIDPAKDVDGFHPLNAGLLATGGSGLVPCTPLGSLMLLKDRLGSLAGLH
                     AVVVGRSNIVGKPMAQLLLREDCTVSVVHSRTREPAALCRQADIVVAAVGRPRMIGPD
                     WVKPGATVIDVGINRIALDDGRTKLVGDVDFDAVAALAGAITPVPGGVGPMTIACLLH
                     NTLTAACRRRGVELPD"
     gene            complement(3716..5842)
                     /locus_tag="PK144_11815"
     CDS             complement(3716..5842)
                     /locus_tag="PK144_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF66462.1"
                     /translation="MIESPAAQPPSPGRPAGSGLRAPAGLGVLGLLLAVWHDEWSVPA
                     AAGIVVLALAAGLALRIAALERAARQALAAELDETRRRLDAIIGQSPSALSLKDLDGR
                     YVCVNGNVERLLQRPAAGILGRTDSELYLPDTARVFREHDRQVLETRRRHSVEESVVV
                     DGEPRLYISHMFPVPDAHGEPALVCRIALDITETRRAQDALVASEARLRAIFAGIGDA
                     AIYCDTGRSIELVNPAFTKIFGYPAQEVIGRSTAMLYADAADFEAAGRVCYHPDMDSD
                     PLPRTLRYRRRDGSLFWSESVGMPVRNDRDELIGYLGVHRDVSARLATERALRDSESR
                     LRLFVEYAPAALAMFDRDMRYLVASHRWRQDYGLGERPLVGVSHYEVFPEIPESWREA
                     HRRGLAGEVLGAPADRFVRVDGSEQWIRWEIRPWRDAGGAVAGIVIFADDISALLRAS
                     EEVRRLNAELEQRVEARTAELRAVNAELDSFAYAVSHDLRAPLRALSGFSNALIEDYG
                     PRLDGEARGFLGEIENASRRMAELIDGLLVLSRCSSGGTLRCEPVDLSALAAAVLARL
                     ARAEPQRQVEVRIEPGLEVCGDPRLLGQVLENLLDNAWKYTARQPSATIRVYAEPGAQ
                     AGERWICVADDGAGFDMANAARLFQPFQRLHRQAEFPGLGIGLATVQRIVRRHGGRIE
                     ARSAPGTGAVFRFTLPGRAALPVEAA"
     gene            complement(5973..6689)
                     /locus_tag="PK144_11820"
     CDS             complement(5973..6689)
                     /locus_tag="PK144_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66463.1"
                     /translation="MNRPAFPFRWQRLPVALLLLVAGCGPQRDPADLFPLERGRAWTY
                     RMQTLNGPDFRRETLRIEALGPRELDGRTVWVRHTSDGTEYYLERDARGLRRVAQRSV
                     LDDLPRWDAPERTVLPAAPAVGQHWSVPGRPYLLHRVRPVVEDLRQTRPFEFEYGIED
                     TDATVEVPAGRFAHCVHVRGEASVYLVADPRFGPQDVPITQDEWYCPGTGLVKLERRE
                     PIEAADVYGGSLTLELEAAR"
     gene            complement(6686..7207)
                     /locus_tag="PK144_11825"
     CDS             complement(6686..7207)
                     /locus_tag="PK144_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66464.1"
                     /translation="MSAAGWGITGAYVLLALLVGSLWLHARLAVWVKLAAVGLVTASY
                     WLVWTTLPGFAGWPAEDELPARFQLLASEIREPDAANGFPGAVHVWVVDLAGDRPAAA
                     PRAYRLAYGKDLHASLDAAKKRIQEGLPQVGRRTDDVRLGVAIDRSRFGERHDELDRL
                     LIENLPDPRLPEK"
     gene            complement(7204..>7814)
                     /locus_tag="PK144_11830"
     CDS             complement(7204..>7814)
                     /locus_tag="PK144_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66465.1"
                     /translation="SLRRKRRDFELDPFTDLLFNALLGITFMFLLAFVAMNPKAKTGT
                     IEAKAEFIITVTWPDNSPDDIDTWVESPDGEVVWFKHPDAGLMHLDRDDRGLVGDQII
                     VDGRVISNPLNEEVVTLRGVVPGEYTVNVQYYESGTRAPVPVEVRVVKVNPHLSVVYY
                     GQLKLEHINSEVTAVRFTVMPDGRVTGINTLAKPLAAVRGLK"
BASE COUNT         1194 a         2859 c         2720 g         1041 t
ORIGIN      
        1 cagcttcgcc tgcgaggagt acgtctactc gctgccggac atcgaggact acatcggcca
       61 gaagatcccg gtcgagccga tcgagcccgg cctgctcgcc gaggtgcgcc cggccaggcg
      121 cagcgagcac cacggcgagc gccgtccgca cggccgtccg ccgcgccgcc ggcacaacgg
      181 cggccgcccg ggctgatacc gatcacgcgt gaatgcgttg ctgatctccc tctccacgcc
      241 cctccccctc aaggggggag gggctgaaaa gaccgggagt tcagccttga tcggcatgag
      301 ctaccagaac agctcgccac gcacgtaggc tgccgcctcg cgcgtggccg gctgcgcgaa
      361 gaaaccggcg gccggcgcgc gttccagcag ccggccgcgg tggaagaaca gcacctcgcc
      421 accgagccgg cgcgcctgcg ccagatcgtg cgtgctcatg acgatgtgcg tgccgcgcgc
      481 gcggaaatcc ccgatcagcg cctcgacctg cgcggcagcg gccggatcga ggctcgcggt
      541 cggctcgtcg aggaacagca catccgggtg cagcgcccag gcccgcgcca gcgccagccg
      601 ctggcgctcg ccgccggaga gcaccggcgc catgcgcgca gccagcgccg tcaggccggc
      661 acgcgccagg gcctcctcgc tgcgccggtg gcgttcggcc ctcgccaggc cgcgcaggcc
      721 gagcgcgtat tcgacgttgg cgcgcaccgg acggcgcagc acgaccgggt gctggaacac
      781 catcgcctga cgctgccggc aggccgcggc ctccgtcgcc cagcgcacct gcccgcccgt
      841 cggctgcagc aggccgtgcg cgaggcgcag gaacaggctc ttgccggcgc cgttggggcc
      901 gagcaccaga tgcacgccgg gtgttgagtc gagcacacag tcgatcggcc cgagcagcgt
      961 gcgcttgccg accgcgtagc acgccgcctc gaagccgagc ggcagcaggc cggccggctc
     1021 agccttccag tcgctgggca cgggtcttca cgacatcggc agccacgttc acgagcagcg
     1081 cgatcaggat caggatcagc ccgagcgcca gcgccagcgg caggtcgccc ttgctggtct
     1141 ccagcgcgat cgccgtggtc atcgtgcgcg tcacgtgcgc gatgttgccg ccgacgatga
     1201 tgatcgcgcc gacctcggac accgagcgcc cgaagccggc gagcacgacg gtcagcaggc
     1261 tccagcgccc ctcccgcagc agcgtcggca ccgcgcgcag cgggctgcag ccgagcgagc
     1321 gcagctgatc gccgagctgc gcccagaggt cctcgacgat ctggcggctc agcgcgacga
     1381 cgatcggcgt gatcagcacc acctgcgcga cgaccatcgc ctcgggccgg aacagccagc
     1441 cgagttcgcc gagcgggccg ctgcgcgaca gcatcagata gacgaacagg ccgacgacca
     1501 ccggcggcat gcccatgaag gcgttggtga tgacgatcag cacgcgccgg cccggaaagc
     1561 gcccgagccc gagcagcgcc ccgaacggca ggccgagcag cgtcgagacc agcaccgcgc
     1621 cggcactgag ctgcaaggac agcacgacga tgccgtagag ctgcgcatcg ccgccggcga
     1681 gcaggtcacc ggccagccag aatgcggcga cgagctcgtt cacgagcgat gccacccgcc
     1741 ggaccttacg ggaacacgac cgtgcgccgg ccgttgagca gcacgcggcg ttcgaggtgg
     1801 gccttgaccg cgcgtgcgag cacgacgttc tcgagatcgc gcccgaccgc gaccagatcg
     1861 tccggcgtca tcgcgtggtt caccggcgcg acctcctgct cgatgatcgg gccttcgtcg
     1921 aggtcggccg tcacgtagtg cgcggtcgcg ccgatgcgct tgacgccgcg ctcgtaggcc
     1981 tgatggtagg gccgcgcgcc cttgaagctc ggcaggaagc tgtggtggat gttgatcgca
     2041 cggccttcga gctcgcggca catggcatcg gagagcacct gcatgtagcg cgcgagcacg
     2101 accagatcgg cctgctcgtg gcggatgatc tccaggatgc gcgactcctg cgccgccttg
     2161 tcgtcgttca tgatcggcag gtagtggaac ggaatgttgt agctggcggc gagctggtag
     2221 aactcgcggt ggttcgagat cacggccgga atctcgacgt tcagcgcccc gacccggtag
     2281 cggtacagca ggtcgttcag gcagtggccg aacttcgaca ccaggatgac catgcgcggc
     2341 ttgatcgcgg tgtcgtggat ctcccagtcc atcgcgaagc gctcgccgat cggcgtgaag
     2401 gccgcgcgca tcgccgtggc atccggcagg cgctcgctgg cgcagaagct cacgcgcagg
     2461 aagaagcggc cggtgtcggc gtcgccgaac tggtcgctct gcacgatgtt gttgccgtat
     2521 tcggcgagga aacccgacac ggcggcgacg atgccggtgg tgtccgggca ggcgagcgtc
     2581 aggatgaaat gggcggcggc gtacggcatg gcggcatctg tccgggcgcc gcggcgcgcc
     2641 ggctcgtggg gcggatgggc tggacgcacg gaccggaagc cacccgcgcg acgtaaacgc
     2701 gcgagcataa gcagcgatcc ctgcacgaac aagcactgcg cagcgctatg atgcggcccg
     2761 ccttccgctc cccgcccccg gatcagcccc aggagacgcc cgcccatggc cgacatcatc
     2821 gacggcaagg cccacgccgc cggcctgcgc gcccatctcg cccgccgtgt cgcgcagctg
     2881 cacgccgatg ccggcttcgt gcccggcctc gccgtcgtgc tggtcggcga ggacccggcg
     2941 agccaggtct acgtgcgcaa caaggccgcg cagacgcgcg aatgcggcat gcagtccatc
     3001 gaacaccgcc tgccggcgag caccacgcag gacgaactgc tggcgctggt cgcgacactg
     3061 aacgccgacc ccgaagtcga cggcatcctc gtgcagctgc cggtgccggc gcatatcgac
     3121 ccggagcgcg tgctgctcgc catcgacccg gccaaggatg tcgacggctt ccacccgctc
     3181 aacgccggcc tgctcgccac cggcggcagc ggcctggtgc cgtgcacgcc gctcggctcg
     3241 ctgatgctgc tgaaggatcg cctcggcagc ctcgccggcc tgcacgcggt cgtggtcggg
     3301 cgctcgaaca tcgtcggcaa gccgatggcg cagctgctgc tgcgcgagga ctgcacggtc
     3361 agcgtcgtgc attcgcgcac gcgcgagccg gcggcactct gccggcaggc cgacatcgtc
     3421 gtcgccgcgg tcggccgccc gcgcatgatc ggcccggact gggtgaagcc cggtgccacc
     3481 gtcatcgatg tcggcatcaa ccgcatcgcg ctcgacgacg gcaggacgaa gctggtcggc
     3541 gatgtcgact tcgacgccgt cgcagcgctc gccggcgcga tcacaccggt gccgggcggc
     3601 gtcggcccga tgaccatcgc ctgcctgctg cacaacacgc tgaccgccgc ctgccggcgg
     3661 cgcggggtcg aactgccgga ctagcagcct gcccgggcgc tgcaccgcgc agcgctcagg
     3721 ccgcctcgac cggcagcgcc gcgcgccccg gcagcgtgaa gcggaacacc gcgccggtgc
     3781 cgggggcgct gcgtgcctcg atgcgtccgc cgtggcggcg cacgatgcgc tgcacggttg
     3841 ccagcccgat gccgagcccc ggaaactccg cctgccggtg caggcgctgg aagggctgga
     3901 acaggcgcgc ggcattggcc atgtcgaagc cggcgccgtc atcggccacg cagatccagc
     3961 gctcgccggc ctgcgcgccg ggctcggcgt agacgcggat cgtcgccgag ggctgcctgg
     4021 ccgtgtactt ccaggcgttg tcgagcaggt tttcgagcac ctgtccgagc aggcgcggat
     4081 ccccgcagac ctccagcccg ggttcgatgc gcacctcgac ctgccgctgc ggctcggccc
     4141 gcgccagccg tgcgagcacg gcggcggcca gcgccgacag gtccacgggc tcgcagcgca
     4201 gcgtgccgcc gctgctgcag cgcgacagca ccagcagacc gtcgatcagc tcggccatgc
     4261 gccggctggc gttctcgatc tcgccgagga aaccgcgcgc ctcgccgtcg aggcgcgggc
     4321 cgtagtcctc gatcagcgca ttgctgaagc cgctcaacgc ccgcagcggc gcgcgcaggt
     4381 catgcgagac cgcgtacgcg aagctgtcga gttcggcgtt gaccgcccgc agctcggccg
     4441 tgcgcgcctc gacgcgctgt tcgagttcgg cgttgagccg gcgcacctcc tccgaggcgc
     4501 gcagcagcgc gctgatgtca tcggcaaaga tgacgatgcc ggcgaccgca ccgccggcat
     4561 cgcgccacgg gcggatctcc cagcggatcc actgctcgct gccgtcgacg cgcacgaagc
     4621 ggtcggccgg cgcgccgagc acctcgccgg ccaggccgcg gcggtgggcc tcgcgccagg
     4681 actcggggat ctccgggaac acctcatagt gcgagacccc gaccagcggc cgctcgccga
     4741 ggccgtagtc ctgccgccag cggtggctcg cgaccagata gcgcatgtcg cgatcgaaca
     4801 tcgccagcgc cgccggcgcg tattcgacga acagccgcag gcgcgattcg ctgtcgcgca
     4861 gcgcccgctc ggtcgccagc cgcgcgctga catcgcggtg cacgccgagg tagccgatca
     4921 gctcgtcgcg gtcattgcgc accggcatgc cgaccgactc gctccagaag agactgccgt
     4981 cgcgccgccg gtagcgcagc gtgcgcggca gcggatcgct gtccatgtcc gggtgatagc
     5041 agacgcgacc ggccgcctcg aagtcggccg catcggcgta gagcatcgcc gtgctgcgcc
     5101 cgatcacctc ctgcgccgga taaccgaaga ttttcgtgaa cgccgggttg accagctcga
     5161 tgctgcgccc ggtgtcgcag tagatcgccg catcgccgat gccggcgaag atcgcgcgca
     5221 gccgcgcctc gctggcaacg agcgcgtcct gcgcgcggcg ggtctcggtg atgtcgagcg
     5281 cgatgcgaca gaccagcgcc ggctcgccgt gcgcatccgg caccgggaac atgtgcgata
     5341 tgtagagccg cggctcgccg tcgacgacga cgctttcctc gaccgaatgc cgcctgcgcg
     5401 tttccagcac ctgccggtcg tgctcccgga acacgcgcgc ggtatcgggc agatacagct
     5461 cgctgtcggt acgcccgagg atgccggccg ccgggcgctg cagcaggcgc tcgacgttgc
     5521 cgttgacgca gacgtagcgg ccgtcgaggt ccttcagcga cagcgccgac ggcgactggc
     5581 cgatgatcgc gtcgagccgg cggcgggttt cgtcgagttc ggcggcgagc gcctgccgcg
     5641 cggcccgctc cagcgccgcg atgcgcagcg ccagcccggc ggcgagcgcc agcacgacga
     5701 tcccggccgc tgcggggacg gaccactcat cgtgccagac ggcgagcagc aggccgagca
     5761 cgcccaggcc ggccggtgcg cgcaggccgg agccggccgg ccggcccggc gacgggggct
     5821 gtgcagcagg ggattcgatc atcgcggact ccggacgcag cctcggcagc gccccgggac
     5881 cgcgtcctcc ctgcctcacg accggggcct cgtccggata gcacagcccg gcgcccgcca
     5941 ccttgcgcgc cgtcaactcc gcagccgatc gttcagcgcg cggcttcgag ttccagcgtc
     6001 agcgagccgc cgtagacatc ggccgcctcg atcggctcgc gccgttcgag cttcacgagg
     6061 ccggtgccgg ggcagtacca ctcgtcctgc gtgatcggca cgtcctgcgg gccgaagcgc
     6121 ggatcggcga ccagatagac gctggcctcg ccgcgcacgt gcacgcagtg ggcgaagcgc
     6181 ccggccggca cctcgaccgt cgcatcggtg tcctcgatgc cgtactcgaa ctcgaacggc
     6241 cgcgtctgcc gcaggtcctc gaccaccggg cgcacgcgat gaagcagata cggccgcccc
     6301 ggtaccgacc agtgctgacc gaccgccggg gcagccggca gcaccgtgcg ctcgggagca
     6361 tcccagcgcg gcaggtcatc gagcacgctg cgctgcgcga cgcgacgcag gccgcgcgca
     6421 tcgcgctcca ggtagtactc ggtgccgtcc gaggtatggc gcacccagac cgtgcgcccg
     6481 tcgagttcgc gcggaccgag ggcttcgatg cgcagcgtct cgcgccggaa gtccgggccg
     6541 ttcagcgtct gcatgcggta ggtccacgcc cgcccgcgct ccagcgggaa caggtcggcc
     6601 ggatcgcgct gcggcccgca gccggcgacg agcagcagca gcgcgaccgg cagccgctgc
     6661 cagcgaaacg gaaaggcagg ccggttcatt tttccggcag ccgcggatcg ggcaggttct
     6721 cgatcagcag gcgatcgagt tcgtcgtgac gttcgccgaa gcgcgaacgg tcgatcgcca
     6781 cgccgaggcg cacgtcatcg gtacgccgcc cgacctgcgg cagcccttcc tggatgcgct
     6841 tcttcgcggc gtcgagcgag gcgtgcagat ccttgccgta ggcgagccgg tacgcgcgcg
     6901 gcgccgccgc cggccggtcg ccggcgagat cgacgaccca gacgtggacc gcgcccggaa
     6961 agccgttcgc ggcgtcgggc tcgcgaatct cgctcgcgag cagctggaag cgcgccggca
     7021 gctcgtcttc ggccggccag ccggcgaaac ccggcagcgt cgtccagacc agccagtaac
     7081 tggccgtgac cagccccacg gccgcgagct tgacccacac cgccagccgc gcatgcagcc
     7141 acaggctgcc gaccagcagc gccagcagca cgtaggcacc ggtgatgccc cagccggcgg
     7201 cgctcacttc agcccccgga ccgctgccag cggcttggcc agcgtgttga tgccggtcac
     7261 gcgcccgtcc ggcatgaccg tgaagcgcac cgcggtcacc tcgctgttga tgtgttcgag
     7321 cttcagctgc ccgtagtaga cgacgctcaa gtgcggattg accttcacga cgcgcacctc
     7381 gaccggcacc ggcgcgcgcg tgcccgattc gtagtactgg acattgaccg tgtattcccc
     7441 gggcacgacg ccacgcagcg tcacgacttc ctcgttcagc ggattcgaga tcacgcgacc
     7501 gtcgacgatg atctgatcgc cgaccaggcc gcggtcgtcg cgatcgaggt gcatcagccc
     7561 ggcatccgga tgcttgaacc agaccacctc gccgtccggg ctctcgaccc aggtgtcgat
     7621 gtcgtcggga ctgttgtccg gccaggtcac cgtgatgatg aactcggcct tggcctcgat
     7681 cgtgccggtc ttcgccttcg ggttcatcgc cacgaaggcg agcaggaaca tgaaggtgat
     7741 gccgagcagc gcgttgaaca gcaggtccgt gaacggatcg agctcgaagt cgcgacgctt
     7801 gcggcgcagg gaca
//
LOCUS       DAOQIE010000184         7811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4843_length_7811_cov_6.918257, whole genome shotgun sequence.
ACCESSION   DAOQIE010000184 DAOQIE010000000
VERSION     DAOQIE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_7811_cov_6.918257"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1154)
                     /locus_tag="PK144_11835"
     CDS             complement(3..1154)
                     /locus_tag="PK144_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNF66466.1"
                     /translation="MEGQGASPRRRLALLIDAENISDAGLVRRLLDLLGRHGEVRIRR
                     AYGDWSGERLRRWREALVQHAIQAMQVFPGAAGKNAADIALVVDAMDLLHSGGVDGFC
                     LLASDSDYTRLVVRLREADRYVIVAGRDSTPLALRAAADLFVAVEPLGEIMPAAVLPS
                     PEQPLPMPKPAPSLEPSARQTTDPVAIPVVAEEEPVLMRLVRKIVPATAETAGPAEAG
                     MPEPLETPPLPVTNTDPKSQAEIRNLLCQALADGQWVTMSALGSALRQLVPGFSTAQF
                     GFPKLWTLILAHGDLIDHRPGASGPIGEVRLTPAAVKPDPSPLLRQALASRAWMTLAA
                     AGTAIRACDPGFDVKRYGHAQLQKLLRAHPQLVECRPASGTVQHVRLRA"
     gene            complement(1159..4050)
                     /gene="glnE"
                     /locus_tag="PK144_11840"
     CDS             complement(1159..4050)
                     /gene="glnE"
                     /locus_tag="PK144_11840"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNF66467.1"
                     /translation="MHELDSVLATVPQPLRPGVALHWQRYCEACARDGIEPVAAGWHA
                     ALVRVWACSDFVAENCTRTPRLLAGLIAGEALAAPDVPGAIGGRVAAALADCSDEAVL
                     SARLRTLRRREMVRIAWRDLSGAAKLAETLEDLSALADACVDAALTWLYDAASRRHGE
                     PRDERGRAQRLVVLGMGKLGGRELNYSSDIDLIFAFPEHGSTAGPRPLENQEFFLRLA
                     QKLIRVLDALTADGFVFRVDMRLRPFGDSGPLVASFAALETYYQSQGRDWERYAMIKA
                     RAMAGDLEAGERLLETLRPFVFRRYLDYGAFESLRNLKAMIAQEVERKGLQDNVKLGP
                     GGIREIEFIGQAFQLIYGGREPPLRVRGIREVLERLADSRRLPEDAVDRLQHAYDCLR
                     RTENRLQAWQDRQTHRLPEDDTGRLRLAFSMGFADWPAFRQTLDAHRSFVAERFAQVF
                     ALGREAPVAEGMAALAAVWHETVSREAAAARLAAAGYDHPPAALEALDRLRGSLAYRS
                     MSAHGRERLDRLMPALLREAGNGPQPAVTLARLAPLLEAVARRSVYLALLHEKPVALG
                     QLVALAGASPWIAEQLTRFPVLLDELLNPAVLYAPPGPEALAAEVRQMLSRVPEGDHE
                     AHLLVLRQFKQINVLRVAAADVSGALPLMVVSDHLTAIAETLLHEALVQAWADVTVRH
                     GLPRCVVDGVARTAGFAIIAYGKLGGLELGYGSDLDLVFLHDSAGEEQETDGERPLDN
                     ATFFARLAQRLIHVLTAPTGAGVLYEIDTRLRPSGRAGMMVASIDGYAAYQRAGAWTW
                     EHQALVRARPVTGSAAIAERFCALRQEVLARVREPAVLRREVREMREKMREHLGSRNP
                     AAFHLKQDRGGMADIEFIVQYLVLAHTAEEPILVRYTDNVRQLAALEATGILRSADAN
                     LLRDAYRQLRRRAHLAKLRDEPATITGDELKPYRDGVERLWQRIMEAED"
     gene            complement(4186..6570)
                     /locus_tag="PK144_11845"
     CDS             complement(4186..6570)
                     /locus_tag="PK144_11845"
                     /inference="COORDINATES: protein motif:HMM:NF017870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HNF66468.1"
                     /translation="MADIMGKAIWAGVGLVAVAVAGVVGGAAWGGREAERQYRDWLAT
                     LEDPQAGLHASSLAYERGLFSATARTRIEWRAPFAAADVPPLALIAEHRVQHGPIAPA
                     DWLAEGRWTPRLGAMRTTLHVDPASAAALPGLRSAPEAARIDSQIGFDGDSTHDVTIA
                     ALDYADAASGDALQLKPLTGRYVLDRQRHLRGELHGDGLTLRFSVAAPPPEAPAVPEL
                     PDAATGAAPSADEGSQTPPPSPQGPLPREPRIVDAGGLKFDFDQTVQAGGYWPGQATI
                     AAQRIAVGDGSGEPLAAFEQPQVRVTLASADGQLYDLREAFSVERVLAHGQNVGPLRF
                     TLVGERLARAALDDLRGFYRTLAQAGGPASIEEPAMQAQLAAIGRKAIAARPRFGFTD
                     IDLRLPGQQPVTGRLLIGLRPPAEDNLPPQAFLREALDTIDLLIDAPALAWGEAGNAG
                     ADSGRIEGLRFTATATPGLASVDADFRLDRLVTHVIGLAGPTDVTLSGLRGDSRQKRG
                     QTTLLIGDANVGLDELHVTGGAGEEPSNLKLSRFTIQVLATEDGEYLGFRERFALEAL
                     ELDGKPQGRGELSFVASRLHGPTLLKLQRAGDEFSTLSPGSTPDPTALIAAMMEFLPH
                     HPVLAFENVHIEKTGEPLPLDARLALTLQPPTDPSAPDAALQYLQAAQGQVAVPESFV
                     LALVTEQVHTAMANEAEMAEMERRMQQEEAGAQADPDRPEAGTEPDTPLPEPDPQQVA
                     ERARQVYDENLAQLLAQGWLKRADGRLSTVIDYRERKLTLNGVELPVAQMLGGE"
     gene            complement(6737..>7811)
                     /gene="groEL"
                     /locus_tag="PK144_11850"
     CDS             complement(6737..>7811)
                     /gene="groEL"
                     /locus_tag="PK144_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNF66469.1"
                     /translation="EGMQFDRGYLSPYFINNQQSMAAELDGPYILLCDKKVSNIRDLL
                     PVLEGVAKSGKPLLIIAEDVEGEALATLVVNTIRGIVKVAAVKAPGFGDRRKAMLQDI
                     AILTGGQVISEEVGLTLEKASLADLGTAKKVVVAKENTTIIDGAGSEAEIKARVQQIR
                     AQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVE
                     EGVVPGGGVALIRALQAIKGVKGDNHDQDVGVAIALRSMEEPLRQIVANAGEEPSVIL
                     NTVAGGEGNYGYNAATGVYGDMIEMGILDPTKVTRSALQNAASVAGLLITTEAMIAEL
                     PKKEEPAAGGMGGMGGMGGMDMM"
BASE COUNT         1054 a         2803 c         2823 g         1131 t
ORIGIN      
        1 gctcaggcgc gcaggcgcac gtgctggacg gtgccgctcg ccggccggca ctcgaccagt
       61 tgcggatggg cacgcagcag cttctgcaac tgggcgtgac catagcgttt cacgtcgaag
      121 ccggggtcac aggcccggat cgcagtgcca gcggctgcaa gcgtcatcca ggcacggctg
      181 gccagcgcct gccgcaacag gggcgacgga tcgggtttga ctgcggcggg tgtcaaccga
      241 acctcgccga tcgggccgct cgcacccggc cggtgatcga tcaggtcgcc atgggccagg
      301 atcagggtcc agagcttcgg aaaaccgaat tgcgccgtgc tgaatcccgg cacgagctgg
      361 cgcagggcac ttccaagggc cgacatcgtg acccactgcc cgtcggccag ggcctgacag
      421 agcaggttgc ggatttcggc ctgcgacttc ggatcggtgt tcgtgacggg caacggaggc
      481 gtctccagag gctccggcat gccggcctct gccggccctg ccgtttccgc ggtcgccggc
      541 acgattttcc gtaccagccg catcaggacc ggctcctcct ccgccaccac cgggatagcc
      601 accggatcag tggtctgacg ggctgacggc tccagtgaag gcgccggttt cggcatcggc
      661 aggggttgct ccggcgacgg cagcaccgct gccggcatga tctcgccgag cggttcgacc
      721 gccacgaaca ggtccgccgc cgcgcgcagc gccagcggcg tcgagtcgcg gccggcaacg
      781 atgacgtagc gatccgcctc gcgcagccgc acgacgagcc gcgtgtagtc actgtcgctg
      841 gcgagcaggc agaaaccgtc cacgcccccg ctgtgcagca ggtccatcgc atcgacgacc
      901 agcgcgatgt cggcggcgtt cttgcctgca gcgcccggga acacctgcat ggcctggatc
      961 gcgtgctgca ccagcgcctc gcgccagcgc cgcaggcgct cgccgctcca gtcgccgtag
     1021 gcgcggcgga tgcggacctc gccgtggcgc ccgagcaggt cgagcagccg gcgcacgaga
     1081 ccggcgtcgg agatgttctc ggcgtcgatc agcagtgcca gccgccggcg cggcgacgct
     1141 ccctgtcctt ccatgccttc agtcctccgc ttccatgatc cgctgccaca gccgctcgac
     1201 gccgtcgcgg tagggtttca gctcgtcgcc ggtgatcgtc gccggctcgt cacgaagttt
     1261 cgccagatgc gcgcggcggc gcagctgccg gtaggcgtca cgcagcaggt tggcgtcggc
     1321 cgagcgcagg atgccggtgg cttcgagcgc ggcgagctgg cggacgttgt cggtgtagcg
     1381 cacgaggatc ggctcctcgg ccgtgtgggc gagcaccagg tactggacga tgaactcgat
     1441 gtcggccatg ccgccgcggt cctgcttcag gtggaaggcg gccggattgc gcgagccgag
     1501 gtgctcgcgc atcttctcgc gcatctcgcg cacctcgcgg cgcagcacgg ccggttcgcg
     1561 cacgcgggcg aggacttcct gacgcagcgc gcagaagcgc tcggcgatcg ccgccgagcc
     1621 ggtcacgggg cgcgcgcgca ccagcgcctg atgctcccag gtccaggccc cggcgcgctg
     1681 gtaggcggcg tagccgtcga tgctcgcgac catcatgccg gcgcgaccgc tcgggcgcag
     1741 gcgggtgtcg atctcgtaga gcacgccggc cccggtcggc gcggtcagca cgtggatcag
     1801 gcgctgcgcg agccgcgcga agaacgtggc gttgtcgagc ggacgctcgc cgtcggtttc
     1861 ctgctcctcg ccggcggaat cgtgcaggaa caccagatcg aggtccgagc cgtagccgag
     1921 ttcgaggccg ccgagcttgc cgtaggcgat gatcgcgaag ccggcggtgc gcgcaacgcc
     1981 gtcgacgacg cagcgcggca ggccgtggcg gacggtgacg tcggcccagg cctgcaccag
     2041 cgcctcgtgc agcagggttt cggcgatcgc ggtcaggtga tcgctgacca ccatcagcgg
     2101 cagtgccccg ctcacgtcgg cggcggcgac gcgcagcacg ttgatctgct tgaactggcg
     2161 cagcaccagc aggtgcgcct cgtgatcgcc ttcgggcacg cgcgacagca tctgccggac
     2221 ctcggcggca agcgcctcgg ggccgggcgg cgcatagagc acggccgggt tcagcagctc
     2281 atcgagcagc accgggaagc gcgtcagctg ctcggcgatc cacgggctgg ccccggcgag
     2341 cgcgaccagc tggcccagcg ccaccggctt ctcgtgcagc agcgccagat acaccgagcg
     2401 gcgcgcgacg gcttcgagca gcggtgccag cctcgccagc gtcaccgccg gctgcgggcc
     2461 gttgccggcc tcgcgcagca gcgccggcat cagccggtcg agccgctcgc ggccgtgcgc
     2521 gctcatcgag cggtacgcga gactgccccg cagccggtcc agcgcttcga gcgcggccgg
     2581 cggatggtca tagccggcgg cggcgagccg cgcggcggcg gcctcgcggc tgaccgtctc
     2641 gtgccagacc gccgccagcg cggccatgcc ttcggcgacc ggcgcctcgc gcccgagcgc
     2701 gaacacctgc gcgaagcgtt cggcaacgaa gctgcggtgc gcatcgagcg tctggcggaa
     2761 ggccggccag tcggcaaagc ccatcgagaa cgccagccgc agccggccgg tgtcgtcctc
     2821 gggcaggcgg tgggtctggc ggtcctgcca ggcctgcagg cggttctcgg tgcggcgcag
     2881 gcagtcgtag gcatgctgca ggcgatcgac ggcgtcctcg ggcagccggc ggctgtcggc
     2941 gagccgctca agcacctcgc ggatgccgcg cacgcgcagc ggcggctcgc gtccgccgta
     3001 gatcagctgg aaggcctgac cgatgaactc gatctcgcgg atgccgccgg ggccgagctt
     3061 gacgttgtcc tgcaggccct tgcgctcgac ctcctgcgcg atcatcgcct tgaggttgcg
     3121 caggctctcg aaggcgccgt aatcgagata acggcggaac acgaagggtc gcagcgtctc
     3181 cagcaggcgc tcgccggctt cgaggtcgcc ggccatcgcc cgcgccttga tcatcgcgta
     3241 gcgctcccag tcgcggccct gcgactggta gtaggtttcg agcgccgcga agctcgccac
     3301 cagcggcccg gaatcgccga acggccgcag gcgcatgtcg acgcggaaca cgaagccgtc
     3361 ggcggtcagc gcatcgagca cgcggatcag cttctgcgcc agacgcagga agaactcctg
     3421 gttctccagc ggccgcgggc cggcggtgct gccgtgctcg gggaaggcaa agatcaggtc
     3481 gatgtcggac gaatagttca gctcgcgccc gccgagcttg cccatgccga gcacgaccag
     3541 ccgctgcgcg cgcccgcgct cgtcgcgcgg ctcgccgtgg cgccggctcg ccgcgtcgta
     3601 cagccaggtc agcgcggcat cgacgcaggc atcggcgagc gcgctcaggt cttccagggt
     3661 ttcggcgagc ttcgccgcgc cggacagatc ccgccaggcg atgcgcacca tctcgcgccg
     3721 gcgcagcgtg cgcaggcgcg ccgacagcac ggcctcatcg gaacaatccg cgagcgccgc
     3781 agccacgcgc ccgccgatcg cgccgggcac gtccggcgct gcgagcgcct ccccggcgat
     3841 cagcccggcg agcaggcgcg gcgtgcgcgt gcagttttcg gcgacgaagt cgctgcaggc
     3901 ccagacccgg accagcgcgg cgtgccagcc ggcggcaacc ggttcgatgc cgtcgcgtgc
     3961 gcaggcctcg cagtagcgct gccagtgcag ggccacgccc ggacgcagtg gctgcggcac
     4021 ggtggccagt acggaatcga gttcatgcat ggtggtgcgc ctcctgccgg cgtctgcacc
     4081 gaaggggtgc atcggcgcgg caggctccag cgtagccgcc ggtgcgcgcg ctggcagccg
     4141 gcgcgtggcc gccggctgac gcaggtcagt gcagggtggc cgggctcact cgccgccgag
     4201 catctgcgcg accggcagct ccacgccgtt cagcgtcagc ttgcgctcgc ggtagtcgat
     4261 gacggtgctc aggcgcccgt cggcacgctt gagccagccc tgcgcgagca gctgcgcgag
     4321 gttttcgtcg tagacctgcc gggcgcgctc ggcgacctgc tgcgggtcgg gttcgggcag
     4381 cggcgtgtcg ggctcggtgc cggcctccgg gcggtccgga tcggcctggg caccggcctc
     4441 ctcctgctgc atgcggcgct ccatctcggc catttcggcc tcgttggcca tggccgtgtg
     4501 cacctgctcg gtaacgaggg ccagcacgaa ggactccggc accgccacct gcccctgcgc
     4561 ggcctgcagg tactgcagcg cggcatccgg cgccgacgga tcggtcgggg gctgcagcgt
     4621 cagggccagc cgcgcatcga gcggcagcgg ctcgccggtc ttctcgatgt gcacgttctc
     4681 gaaggcgagc accgggtggt gaggcaggaa ttccatcatc gccgcgatca gggccgtcgg
     4741 atcgggcgtg ctgccggggc tcagggtcga gaactcgtcg ccggcgcgct gcagcttcag
     4801 cagcgtcggc ccgtgcaggc gcgaggcgac gaagctcagc tcgccgcggc cctgcggctt
     4861 gccgtcgagc tccagcgcct ccagcgcgaa gcgttcgcgg aagccgaggt attcgccgtc
     4921 ctcggtggcg agcacctgga tcgtgaagcg gctgagcttc aggttgctcg gctcctcgcc
     4981 ggcaccgccg gtcacgtgca gctcgtcgag tccgacattg gcatcgccga tcagcagcgt
     5041 cgtctggccg cgcttctggc ggctgtcgcc gcgcaggccg ctcagcgtca cgtcggtcgg
     5101 gccggcgagg ccgatgacat gggtgaccag ccggtcgagc cggaaatcgg catcgacgct
     5161 cgcgagcccc ggcgtggcgg tcgccgtgaa gcgcaggccc tcgatgcggc cggaatcggc
     5221 gccggcgttg ccggcctcgc cccatgcgag cgccggggcg tcgatcagca ggtcgatcgt
     5281 gtcgagcgcc tcgcgcagga aggcctgcgg cggcagattg tcctcggccg gcgggcgcag
     5341 gccgatcagc agccggccgg tgaccggctg ctggccgggc aggcgcaggt cgatgtcggt
     5401 gaagccgaag cgcggccggg ccgcgatcgc cttgcgcccg atcgccgcga gctgcgcctg
     5461 catggccggc tcctcgatcg aagccgggcc gccggcctgc gccagcgtgc ggtagaagcc
     5521 gcgcaggtca tcgagcgcgg cgcgcgccag ccgctcgccg accagcgtga agcgcagcgg
     5581 cccgacgttc tggccgtgcg cgagcacgcg ctcgaccgag aaggcctcgc gcaggtcgta
     5641 gagctggccg tcggcactcg ccagcgtcac gcgcacctgc ggctgctcga aggcggcgag
     5701 cggctcgccg ctgccatccc cgaccgcgat gcgctgcgcg gcgatcgtcg cctgcccggg
     5761 ccagtagccc ccggcctgca cggtctggtc aaaatcgaac ttcagcccgc cggcatcgac
     5821 gatgcgcggc tcgcgcggca gcggcccctg cgggctcggc ggcggcgtct ggctgccttc
     5881 atcggccgaa ggggccgcac cggtggcggc atcgggcagc tcgggcaccg ccggcgcttc
     5941 gggcggcggg gcggcgacgc tgaagcgcag cgtcaggccg tcgccgtgca gttcgccgcg
     6001 cagatgccgc tggcggtcga gcacgtagcg gccggtgagc ggcttcaact gcagcgcgtc
     6061 accgctggcg gcatcggcgt agtcgagcgc ggcgatcgtc acgtcgtgcg tactgtcgcc
     6121 atcgaaaccg atctgcgaat cgatgcgcgc cgcctcggga gcgctgcgca gccccggcag
     6181 cgccgcggca ctggccggat cgacgtgcag cgtcgtgcgc atcgcgccga ggcgtggcgt
     6241 ccagcggcct tcggcgagcc agtcagccgg tgcgatcggg ccgtgctgga cgcggtgttc
     6301 ggcgatcagc gccagcggcg gcacatcggc ggcggcgaac ggcgcccgcc attcgatgcg
     6361 cgtgcgcgcc gtggcgctga acaggccgcg ctcgtaggcg aggctgctcg cgtgcaggcc
     6421 ggcctgcgga tcctccagcg ttgcgagcca gtcgcggtac tggcgttccg cctcgcgccc
     6481 gccccacgcc gccccgccga cgacgccggc cacggccacc gccaccagcc ccacgcctgc
     6541 ccagatcgcc ttgcccatga tgtccgccat gcctcgatgc ggcgccctcc gccgcgcagg
     6601 ccgcgagtat tccacacgcc ggcggagcct gcggtacggc accggccgca ggcagccccg
     6661 aaaagacaga gccccgccga agcggggctc tggtcaggcg aggcggccgc acgcgggcgg
     6721 ccgccggcgc acggacttac atcatgtcca tgccgcccat gccacccatg ccgcccatgc
     6781 caccggcggc cggctcttcc ttcttcggca gctcggcgat catggcttcg gtggtgatca
     6841 gcaggccggc gaccgaggcg gcgttctgca gcgccgaacg ggtgaccttg gtcggatcga
     6901 ggatgcccat ctcgatcatg tcgccgtaga cgccggtcgc ggcgttgtag ccgtagttgc
     6961 cctcaccgcc ggcgacggtg ttcaggatca ccgacggctc ctcgccggcg ttggcgacga
     7021 tctggcgcag cggctcttcc atcgagcgca gcgcgatcgc gacgccgacg tcctgatcgt
     7081 ggttgtcgcc cttgacgccc ttgatcgcct gcagcgcgcg gatcagcgcg acgccgccgc
     7141 ccgggaccac gccttcctcg accgcagcgc gcgtggcgtg cagcgcgtcc tcgacgcggg
     7201 ccttcttttc cttcatctcg acttcggtcg cggcaccgac cttgatcacg gccacgccgc
     7261 cggccagctt ggcgacgcgc tcctgcagct tctcgcggtc gtagtcggag gtggcttcct
     7321 cgatctgcgc gcggatctgc tggacgcggg ccttgatctc ggcctcggaa ccggcgccgt
     7381 cgatgatcgt ggtgttttcc ttggcgacga cgaccttctt ggcggtgccg aggtcggcga
     7441 gcgaggcctt ctccagcgtc aggccgactt cctcggagat cacctggccg ccggtcagga
     7501 tcgcgatgtc ctgcagcatg gccttgcggc ggtcaccgaa gcccggcgcc ttgacggcgg
     7561 cgaccttgac gatgccgcgg atggtgttga cgaccagcgt cgccagcgcc tcgccctcga
     7621 catcctcggc gatgatcagc agcggcttgc cggacttggc gacgccttcg agcaccggca
     7681 gcaggtcacg gatgttcgag accttcttgt cgcagagcag gatgtacggg ccgtcgagct
     7741 cggcggccat cgactgctgg ttgttgatga agtacggcga caggtagccg cgatcgaact
     7801 gcatgccctc g
//
LOCUS       DAOQIE010000185         7810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4844_length_7810_cov_8.154997, whole genome shotgun sequence.
ACCESSION   DAOQIE010000185 DAOQIE010000000
VERSION     DAOQIE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4844_length_7810_cov_8.154997"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PK144_11855"
     CDS             <1..707
                     /locus_tag="PK144_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF66470.1"
                     /translation="ALHDASTIAAVIVEPVAGSTGVLIPPQGYLERLRAICDRHGILL
                     IFDEVITGYGRTGAPFAFQRLGVTPDLVTTAKGLTNGTVPMGAVLCRPGIYETFMQGP
                     PAAIELFHGYTYSGHPLACAAGLATLDVYAEENLLTRALELEDDFATALHDLRGLPNV
                     VDIRNLGLVGAVELAPRPGAPGARASEAFLECYRRGLLVRYTGDTLAFSPPLVISRAQ
                     IGEIFAGVRAVLAELA"
     gene            774..2276
                     /locus_tag="PK144_11860"
     CDS             774..2276
                     /locus_tag="PK144_11860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNF66471.1"
                     /translation="MSIREISHWIGGHCVPGTSGRSGPVFDPARGVQTGSVAFASVEE
                     VDAAVQAARAAFPAWAATPPLARARVMFRFRELLEARAAQIAALISSEHGKVISDARG
                     ELTRGIEVVEFACGIPQLLKGEFSGNVGSAVDSTALRQPLGVVAGITPFNFPAMVPMW
                     MFPVAIASGNTFVLKPSERDPGTAGLLAELLAEAGLPAGVFNVVHGDRIAVDALLDHP
                     DVAAVSFVGSTAIARHVYARGCAAGKRVQALGGAKNHLVVMPDADLEQAVDALIGAAY
                     GSAGERCMAISVAVAVGEATADALVARLAPRVAGLRVGPGDDALAEMGPLVSAEHRAR
                     VAGYIARGIEEGAEAVVDGRGLRIEGCEDGFFLGGTLFDRVRPDMAIWREEIFGPVLA
                     IVRVPDYAAALALVNSHEYGNGAALFTRDGDTARHFTEHVSAGMVGINVPIPVPMAFH
                     SFGGWKASLFGDHHVHGPEGVRFYTRLKTVTSRWPTGIRAGAQFAMPTLG"
     gene            2525..3682
                     /locus_tag="PK144_11865"
     CDS             2525..3682
                     /locus_tag="PK144_11865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF66472.1"
                     /translation="MSAVRSIADHLFAPLRVQSTDIADLFGVWNHSSQLRRQRRVVLK
                     SRTRLMAALFALLVPLWILPDLLLLDTDHWLPLAGLRVASAVVFMGLAWSRDPGCGRL
                     EALLRVGLLLAVPTVFHLLAAPLLADLDTGGPRGVLLQVYELLPFVVVAGLAVFPLTV
                     LETLLYALPIFMAYLYGVHINGEFRFAENVGTLWLLLLVTGAAMVSSLSQLQYMIALV
                     RRASADTLTGALNRAAGEETLELLFGMAVARGEPLALMFIDLDRFKSINDNWGHEAGD
                     TVLRAMAGQVRTALRRGDVLVRWGGEEFLVLLANSDVHGARLVVERLRAAGFGERPDG
                     APLTASIGLAERLIDRVSDWPELIEIADQRMYAAKLGGRDRAILPGETIVR"
     gene            3779..6487
                     /locus_tag="PK144_11870"
     CDS             3779..6487
                     /locus_tag="PK144_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66473.1"
                     /translation="MAVPLKLLVIEDVETDFLLAVRVLTRHGYAPQATRVDRLDALEA
                     ALAEGGWELAISDYNLPGLSFDASFARVCAQPGLPLILLSGSVGEERAVELLKSGVAD
                     FVLKSNLGRLPPAVDRCLRERAESRARAEAESALADYRAQLESLVATRTEELRQRERD
                     LQAILDNVPALIGYWDRDLRNRFANQAYHDWLGIPAGGMHGMHKREALGPVRYERVHT
                     AVETALRGEPGLIENEFPHADGSCRYVQTHYLPDLRDGEVHGVYVLVSDVTELKRAEQ
                     AAEAARLDAERLARVKADFLANMSHEIRTPLNAVLGFAELGLDQSEGRRVHETLRRIH
                     DAGQLLLGIVDDILDYSKIEAGKLTLEDAPFAPGAVVDRVIDVIAGRARERGLELCID
                     EAPDLPQQSRGDALRLAQVLLNLMSNAVKFTERGGCVTLSVRHDDDGFTFGVTDTGIG
                     MAAGQLARLFQPFEQADGSITRRFGGTGLGLAICARLTALMGGRIRVDSRCGAGSRFE
                     VCVPLPALPAPPPVLPDGAVAILGLTPAESTALIGALAVHGVTGLQVGADAPLPAAVG
                     ALVAARGADQGRALLGRAAGRRLVLVGAPVHDSPALLRGIPCVDRPLRPRQVLAALAS
                     AVPGPSTPLPGVSRQPRLAGLHLLVIEDNETNRLLLEAMLEREGARLTLAVNGLRALA
                     SLTALGPAQFDLVLTDLQMPEMDGYETARRIAALAPDLPVLALSAHALPDERERCLVA
                     GMKEQLTRPVSADALVDAVLRYCRRPPGVPAVPPAAAPERAVTEARAAAGPAPLDWAA
                     IEADFPGRPDFLRRLIDTVLASNAGVPARIAEAQAAQDLGALGFVAHSLKGMSGTLHA
                     GALRALATRVETLARRGDAGAWALCEALEGALAELLDALRARRRTL"
     gene            6496..7383
                     /gene="ehuB"
                     /locus_tag="PK144_11875"
     CDS             6496..7383
                     /gene="ehuB"
                     /locus_tag="PK144_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033294 - ectoine binding [Evidence IEA];
                     GO_function: GO:0033295 - hydroxyectoine binding [Evidence
                     IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="HNF66474.1"
                     /translation="MDTPGSSAPAGGLRRPVLIVAALLILGAAGLAMLALLPGEEDAW
                     TRIRRSGSVRIGHTAEAPYAWRTPDGRVTGEAPELARAVFAQLGIQHIEWVEVQFGSL
                     IPQLRTGRFDVIAAGMYVTPARERQIAFSHPSYCTHAALLVRHADAQRLRGYADLVQS
                     PGARLAVLAGAVEGEAALAAGVPAGRILAFPDPASAAQAVADGEAEALALSAPSVQWL
                     ADRDPRLRRAAEAVTAAPPEGCGAFGFRQEDRELRRRFDAQLRAFLGTPAHAALVRPF
                     GFTPAELPPALQPAPERRP"
     gene            7380..>7810
                     /locus_tag="PK144_11880"
     CDS             7380..>7810
                     /locus_tag="PK144_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66475.1"
                     /translation="MSGRLQGLGWGERAVLLAALLVLVSGGLLWGVGSASTRTYTQYI
                     ASTDSLGRARQALTEARLGLARLSAREPGLDRNVVLAPLVRAQQAARAALAGQSGLTT
                     LAASQPPAGELAAEFAEFIRALDAFGLAALDADGLGAPGTAL"
BASE COUNT          947 a         2875 c         2855 g         1133 t
ORIGIN      
        1 tggcgctgca cgatgcctcg accatcgccg cggtcatcgt cgagccggtc gccggttcca
       61 ccggcgtgct gattccgccg cagggctatc tggagcgcct gcgcgcgatc tgcgaccgcc
      121 acggcatcct gctgatcttc gacgaggtca tcaccggcta cggccgcacc ggcgcgccgt
      181 tcgcgttcca gcgcctcggc gtgacgccgg acctcgtgac caccgccaag ggcctgacca
      241 acggcaccgt gccgatgggc gcggtgctct gccggcccgg catctacgaa accttcatgc
      301 agggaccgcc ggcggcgatc gagctgttcc acggctacac ctactcgggc catccgctcg
      361 cctgcgccgc cgggctggcg acgctcgatg tctacgccga agaaaacctg ctgacgcgcg
      421 cgctcgaact ggaggacgac ttcgccaccg cgctgcacga cctgcgcggg ctgccgaacg
      481 tcgtcgacat ccgcaacctc ggcctggtcg gggccgtcga gctggcgccg cgccccggcg
      541 caccgggcgc gcgcgccagc gaggccttcc tcgagtgcta ccgccgcggc ctgctggtgc
      601 gctacaccgg cgacacgctc gccttcagcc cgccgctggt catcagccgc gcgcagatcg
      661 gcgagatctt cgccggcgtg cgcgcggtgc tggccgagct ggcctgaggc gcgggccttc
      721 cgcctcacct ccgtcttcct cacatccgtc tttgtccccc cgaggttcgt gcgatgagca
      781 tccgcgagat ttcccactgg atcggcggcc actgcgtgcc cggcaccagc gggcgcagcg
      841 gcccggtctt cgacccggcg cgcggcgtgc agaccggctc ggtggcgttc gcgtcggtcg
      901 aggaggtcga cgccgccgtg caggccgccc gcgcggcctt cccggcctgg gcggcgacgc
      961 cgccgctggc gcgtgcgcgc gtgatgttcc gcttccgcga gctgctcgaa gcccgcgccg
     1021 cgcagatcgc ggcgctgatc tcgtccgagc acggcaaggt gatcagcgat gcgcgcggcg
     1081 agctgacgcg cggcatcgag gtcgtcgagt tcgcctgcgg catcccgcag ctgctcaagg
     1141 gcgagttctc cggcaacgtc ggcagcgcgg tcgattcgac ggcgctgcgc cagccgctcg
     1201 gcgtcgtcgc cggcatcacg ccgttcaact tcccggcgat ggtgccgatg tggatgttcc
     1261 cggtggcgat cgccagcggg aacaccttcg tgctgaagcc ctccgagcgc gatcccggca
     1321 ccgccggcct gctcgccgag ctgctcgccg aggccgggct gccggccggc gtgttcaacg
     1381 tcgtgcatgg cgaccggatc gccgtcgatg cgctgctcga tcacccggac gtggccgcgg
     1441 tcagcttcgt cggctcgacc gcgatcgccc ggcacgtcta cgcgcgcggc tgcgcggccg
     1501 gcaagcgcgt gcaggcgctc ggcggtgcca agaaccatct ggtcgtgatg ccggatgccg
     1561 acctcgaaca ggcggtcgat gcgctgatcg gcgccgccta cggctcggcc ggcgagcgct
     1621 gcatggcgat ctcggtggcg gtcgcggtcg gcgaggccac cgccgatgcg ctggtcgcac
     1681 ggctggcacc gcgcgtcgcc ggcctgcgcg tcggtcccgg ggacgatgcg ctggccgaga
     1741 tgggaccgct ggtcagcgcc gaacaccgcg cgcgcgtcgc cggctacatc gcccgcggca
     1801 tcgaggaggg cgccgaggcg gtcgtcgatg gccgcggcct gcgcatcgag ggctgcgagg
     1861 acggcttctt cctcggcggc acgctgttcg accgcgtgcg cccggacatg gcgatctggc
     1921 gcgaggagat cttcggcccg gtgctcgcga tcgtgcgcgt gccggactac gcggccgcac
     1981 tggcgctggt caacagccac gagtacggca acggcgcggc gctgttcacg cgcgatggcg
     2041 acaccgcgcg ccacttcacc gagcacgtca gcgccggcat ggtcggcatc aacgtgccga
     2101 tcccggtgcc gatggccttc cacagcttcg gcggctggaa ggcgagcctg ttcggtgacc
     2161 accacgtgca cggtcccgag ggcgtgcgct tctatacccg gctgaagacg gtcaccagcc
     2221 gctggccgac cggcatccgc gccggtgcgc agttcgcgat gccgacgctc ggctgaacca
     2281 tcccgcacac aattgcgacg ctgcttgcgt cgggttcgca tttgcgaagc cggcacgaat
     2341 ggccggaaac attgatcctg agcaatgcag gcgacggtgt gctcccgcat cgtgcatgac
     2401 cgtcgcatca cggggccgtg atgcgcaatg gccgtggttg cggcttgcga atcaccgtgc
     2461 cccggaccgg tcaggaaacc ggtgatccgc ggggcgcacc accgaccccc cgaggcccct
     2521 gccgatgtct gcggtccgct cgatcgccga tcacctgttt gcgccgctgc gcgtgcagtc
     2581 gaccgacatc gccgacctgt tcggcgtctg gaaccattcc tcgcagctgc gccgccagcg
     2641 gcgcgtggtg ctgaagtcgc gcacgcggct gatggccgcg ctgttcgcgc tgctggtgcc
     2701 gctgtggatc ctgcccgacc tgctgctgct cgacaccgac cactggctgc cgctcgccgg
     2761 cctgcgcgtg gcgagtgcgg tggtgttcat ggggctcgcg tggtcgcggg acccgggctg
     2821 cgggcggctg gaggcgctgc tgcgcgtcgg cctgctgctg gcggtgccga ccgtgttcca
     2881 cctgctcgcg gcgccgctgc tcgccgatct cgataccggc ggcccgcgcg gcgtgctgct
     2941 gcaggtctac gagctgctgc ccttcgtcgt cgtcgccggg ctggcggtgt ttccgctgac
     3001 ggtgctggag acgctgctgt atgcgctgcc gatcttcatg gcctatctgt acggcgtgca
     3061 catcaacggc gaattccgct tcgccgagaa cgtcggcacg ctgtggctgc tgctgctcgt
     3121 gaccggcgcg gcgatggtgt cgagcctgag ccagctgcag tacatgatcg cgctggtgcg
     3181 ccgggccagc gccgacacgc tgaccggggc cctgaaccgc gccgccggcg aggaaacgct
     3241 cgaactgctg ttcggcatgg cggtcgcgcg cggcgagccg ctggcgctga tgttcatcga
     3301 tctcgaccgc ttcaagagca tcaacgacaa ctggggtcac gaggccggcg acacggtgct
     3361 gcgcgcgatg gccgggcagg tgcgcacggc gctgcgccgc ggcgacgtgc tggtgcgctg
     3421 gggcggcgag gaattcctcg tgctgctcgc caacagcgac gtgcacgggg cgcggctggt
     3481 ggtcgagcgg ctgcgcgcgg ccggcttcgg cgagcggccc gacggcgcgc cgctgacggc
     3541 gagcatcggg ctcgccgagc gcctgatcga ccgcgtcagc gactggccgg agctgatcga
     3601 gatcgccgac cagcgcatgt acgcggccaa gctcggcggg cgtgaccggg cgatcctgcc
     3661 cggcgagacc atcgtccgct aagctgctcc ggtcgttgcc tcaaagccca ctcaagggct
     3721 agggttatgc ggctccccgc cgggacgcaa gccttcgagg cccacgggag cccacgtctt
     3781 ggccgtaccg ctgaagctgc tggtcatcga ggatgtcgaa accgacttcc tgctcgccgt
     3841 gcgcgtgctg acgcgccacg gctatgcgcc gcaggccacg cgcgtcgatc gcctcgatgc
     3901 cctcgaagcc gcgctcgccg agggcggctg ggagctggcg atctcggact acaacctgcc
     3961 gggcctgagc ttcgatgcca gtttcgcgcg cgtgtgcgcg cagcccggcc tgccgctgat
     4021 cctgctctcg ggcagcgtcg gcgaggagcg ggcggtcgag ctgctgaagt ccggcgtcgc
     4081 cgacttcgtg ctcaagagca acctcggccg cctgccgccg gccgtcgacc gctgcctgcg
     4141 cgagcgcgcc gaatcgcgcg cgcgtgccga ggccgaaagc gcgctggcgg actaccgcgc
     4201 gcagctcgaa tccctggtcg cgacccgcac cgaggagctg cgccagcgcg agcgggacct
     4261 gcaggccatc ctcgacaacg tgccggcgct gatcggctac tgggaccgcg acctgcgcaa
     4321 ccgcttcgcc aaccaggcct accacgactg gctcggcatt ccggccggcg gcatgcacgg
     4381 catgcacaag cgcgaggccc tcggcccggt gcgctacgag cgcgtgcaca ctgccgtcga
     4441 gacggcgctg cgcggcgagc ccggcctgat cgagaacgaa ttcccgcacg ccgacggcag
     4501 ctgccgctac gtgcagacgc attacctgcc ggacctgcgc gacggcgagg tccacggcgt
     4561 gtacgtgctg gtcagcgacg tgaccgaact gaagcgcgcc gagcaggccg ccgaggccgc
     4621 ccggctcgat gccgagcggc tcgcgcgcgt caaggccgac ttcctcgcca acatgagcca
     4681 cgagatccgc acgccgctca acgccgtgct cggcttcgcc gaactcgggc tcgaccagtc
     4741 cgagggccgg cgcgtgcacg agacgctgcg ccgcatccat gacgccggcc agctgctgct
     4801 cggcatcgtc gatgacatcc tcgactactc gaagatcgag gccggcaagc tgacgctgga
     4861 ggatgcgccg ttcgcgcccg gggccgtcgt cgatcgcgtc atcgacgtga tcgccggccg
     4921 cgcgcgcgag cgcgggctgg agctttgcat cgacgaggcg ccggacctgc cgcagcagag
     4981 ccgtggcgat gcgctgcgtc tggcgcaggt gctgctgaac ctgatgtcga acgccgtgaa
     5041 gttcacggag cgcggcggct gcgtgacgct cagcgtgcgc cacgacgacg acggcttcac
     5101 cttcggcgtc accgacaccg gcatcggcat ggccgccgga cagctcgcgc ggctgttcca
     5161 gcccttcgag caggctgacg gcagcatcac gcgccgcttc ggcggcaccg gcctcgggct
     5221 ggcgatctgc gcgcggctga cggcactgat gggcgggcgc atccgcgtcg acagccgctg
     5281 cggggccggc agccgcttcg aggtctgcgt gccgctgccg gcgctgccgg cgccgccgcc
     5341 ggtgctgccg gacggcgcgg tggcgatcct cggcctgacg ccggccgaaa gcacggcgct
     5401 gatcggcgca cttgccgtgc acggcgtcac cggcctgcag gtcggtgccg atgcgccgct
     5461 gccggcggcc gtcggcgccc tggtcgccgc gcgcggtgcc gatcaggggc gtgcgctgct
     5521 cggccgcgcc gccggtcgcc ggctggtgct ggtcggggcg ccggtgcacg actcgccggc
     5581 gctgctgcgc ggcattccct gcgtcgaccg gccgctgcgc ccgcgccagg tgctcgccgc
     5641 gctcgccagc gccgtgccgg ggccgtccac gccgctgccc ggcgtctccc gccagccgcg
     5701 gctggccggc ctgcacctgc tcgtgatcga ggacaacgaa accaaccgcc tgctgctcga
     5761 agccatgctc gagcgcgagg gtgcgcggct gacgctggcc gtgaacgggc tgcgggcgct
     5821 ggccagcctc acggccctcg gcccggcgca gttcgatctg gtgctgaccg acttgcagat
     5881 gccggagatg gacggctacg agaccgcccg gcgcatcgcc gcgctcgctc ccgatctgcc
     5941 ggtgctcgcc ctcagtgcgc atgcgctgcc ggacgagcgc gagcgctgcc tggtcgcggg
     6001 catgaaggaa cagctgacgc gcccggtcag cgccgatgcg ctggtcgacg ccgtgctgcg
     6061 ctactgccgc aggccgccgg gggtgcccgc cgtcccgccg gccgcggcgc cggagcgggc
     6121 cgtgaccgag gccagggctg ctgccggacc ggccccgctc gactgggcgg cgatcgaggc
     6181 cgacttcccg gggcgccccg acttcctgcg ccggctcatc gacaccgtgc tcgccagcaa
     6241 cgccggcgtg ccggcgcgca tcgccgaggc gcaggccgcg caggatctcg gcgcgctcgg
     6301 tttcgtcgcg cacagcctca agggcatgag cggcacgctg cacgccgggg cactgcgcgc
     6361 gctcgcgacc cgggtcgaga ccctggcgcg gcgcggcgat gccggggctt gggcgctgtg
     6421 cgaggcgctc gaaggggcgc tggccgaact gctcgacgcg ctgcgggcgc ggcgacggac
     6481 gctgtagagc cctccttgga cacgcccggg tcttccgcgc cggccggcgg cctgcgccgg
     6541 ccggtgctga tcgtggccgc gctgctgatc ctgggcgcgg ccgggctcgc gatgctggcg
     6601 ctgctgcccg gcgaggagga cgcctggacg cgcatccggc gcagcggcag cgtgcgcatc
     6661 ggccacacgg ccgaagcccc gtatgcgtgg cgtacgcccg acggccgcgt caccggcgag
     6721 gcgcccgaac tcgcgcgcgc cgtgttcgcg cagctcggca tccagcacat cgaatgggtc
     6781 gaggtgcagt tcggctcgct gatcccgcaa ttgcgcacgg ggcgcttcga tgtcatcgcc
     6841 gccggcatgt acgtgacccc ggcgcgcgaa cggcagatcg ccttcagcca cccgagctac
     6901 tgcacgcacg ccgcgctgct ggtgcgtcac gccgatgcgc agcgcctgcg cggctatgcc
     6961 gatctggtgc agtcccccgg cgcgcggctc gcggtgctcg ccggcgcggt cgagggtgag
     7021 gccgcactcg ccgccggcgt gccggccggg cgcatcctcg cctttcccga tcccgcgagt
     7081 gccgcgcagg cggtggccga tggcgaggcc gaggcgctgg cgctgtcggc gccgagcgtg
     7141 cagtggctgg ccgatcgcga tccgcggctg aggcgcgccg ccgaagccgt caccgcggcg
     7201 ccgcccgagg gctgcggcgc cttcggcttc cggcaggagg accgggaact gcgccgccgc
     7261 ttcgatgcgc agctgcgcgc cttcctcggc acgcccgcgc atgcggcgct ggtgcggccg
     7321 ttcggcttca cgccggccga actgccgccg gcgctgcagc cggcgccgga gcgccggccg
     7381 tgagcggacg gctgcaaggc ctcggctggg gcgagcgtgc ggtgctgctc gcggcgctgc
     7441 tggtgctggt ctcgggcggc ctgctgtggg gcgtcggcag cgcctcgacg cgcacctaca
     7501 cgcagtacat cgccagcacc gacagcctgg gccgcgcccg tcaggcgctg accgaggcgc
     7561 ggctggggct ggcgcggctg tcggcccgcg agccggggct cgatcgcaac gtcgtgctgg
     7621 cgccgctggt gcgcgcgcag caggcggccc gcgccgctct ggccggccag agcggactga
     7681 cgacgctggc cgcatcgcag ccgcccgcgg gcgagctggc cgcggagttc gcggagttca
     7741 tccgtgcgct cgatgccttc gggctggccg cgctcgatgc cgacggactc ggcgcgccgg
     7801 gcacggcgct
//
LOCUS       DAOQIE010000186         7790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4859_length_7790_cov_7.241888, whole genome shotgun sequence.
ACCESSION   DAOQIE010000186 DAOQIE010000000
VERSION     DAOQIE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_7790_cov_7.241888"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..701)
                     /locus_tag="PK144_11885"
     CDS             complement(69..701)
                     /locus_tag="PK144_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNF66476.1"
                     /translation="MFENIQHWIAEYGYLTILIWTFIEGETVVIVAGVIAKLGHLELP
                     LVMLSAFVGSLCGDQFYFFIGRKQGRKLLDKHPKWAGRVAWINRHLERHRDFLVLTFR
                     FYYGVRNITPFVIGASDISTVRFIVLNMIGAAVWAISFAGAGYLFGEAMETFLGRMHN
                     AQLYILGTIIGLAVLAWLFMRLRRRAKDRQIEADAVVQAPEAKSPPAPDA"
     gene            complement(835..2262)
                     /locus_tag="PK144_11890"
     CDS             complement(835..2262)
                     /locus_tag="PK144_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNF66477.1"
                     /translation="MPGSNPLLRVLLTCLVLLAAGRAPAVELPDFTTLVEQYAPAVVS
                     VSARTAAKDAADEPDEDKPGKPPVQNPFEGTPFEEPFRRFFEQMPHGNSPRPRELPAS
                     IGSGFVISADGYVLTNAHVVEGADTVTVGLRDRTELIAKVIGADKRSDVALLKIDGAA
                     PLPVVRLGDAEQLKVGQWVLAIGSPFGFESSATAGIVSALGRSLPSDSYVPFIQTDAA
                     VNPGNSGGPLFNFNGEVVGINSQIYSRSGGYQGLSFAIPINVAMDVAEQLKTKGRVAR
                     GWLGVLIQEVTPDLAKSFGLDRARGALIGQVLPEGPALKAGLKPGDIVLAFNGRPLLR
                     SSELPPLVGSYLPGKDATLSVFRDGRETTITVKIEELPDDARQAGKAGSGTRRGLGLT
                     LGELPPEAARKGETGVVVREVAPGPAARAGIRTGDVIVRLDNQPVTGVAQFEELAAKL
                     GKGKPVPVLVRRGESPLFLALTPPE"
     gene            complement(2297..3466)
                     /locus_tag="PK144_11895"
     CDS             complement(2297..3466)
                     /locus_tag="PK144_11895"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF66478.1"
                     /translation="MPPTSRPPPERRPTTLLLALFCGSIPDPAHLQGADWEQLLPYAR
                     RHEALGRLATVLAARGVLDRVPARVRFHLDSARVRAQSRMRRLRWEALNLRLVLPGVP
                     VLVLKGVACALRGLPSVQGRLSADLDVLVPKARIAEAEAGLLAGGWEHAAHSDYDDAY
                     YRAWMHELPAFHHAHRGTQLDLHHGILPPTARLHPDPALLLAAAEPVPGVPDLYVPCA
                     SDLLLHAAVHACYDGAFAQPLRDLLDLHGLITDLGDETFWQALPARAVALDLARPLWY
                     ALTLVQRELGTPVPAAVFTALAAHAPPPQLAGVLPALVHAVLHERPMAPLAALALYIR
                     SHWLRMPPRLLLPHLARKAVQRLRTRLGVAAPGAPAAAVEVATPAEPPRPPPGQP"
     gene            3710..4099
                     /locus_tag="PK144_11900"
     CDS             3710..4099
                     /locus_tag="PK144_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66479.1"
                     /translation="MQDHEHSGTDGQDAAAPVSLRRRRLLKSAAVVAVPAVLTLHAGY
                     ARAANSSAVRCALNTVNGTGIRITNENTPGLYKEMGADSQLHAVWFQIDPSSGGITPV
                     HYNETGYKVGTNASFLSSGCVNSALGI"
     gene            4104..4448
                     /locus_tag="PK144_11905"
     CDS             4104..4448
                     /locus_tag="PK144_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66480.1"
                     /translation="MHGTPEPRHPAAFWRPNAFSRLHWQASDEAGEFHLVHDETSGRT
                     HFLTPLGAWLLQRLAEAPAGCAQLQLELRADCDDPDAPGLDQALADALDLLAAELLIE
                     PCPPSWPPASAS"
     gene            4424..5368
                     /locus_tag="PK144_11910"
     CDS             4424..5368
                     /locus_tag="PK144_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNF66481.1"
                     /translation="MAACLGELSRAELRARLADNALRLRIGPFAYALGSEWPGLADWL
                     APLYARYPRFPDDECADFRVRLRWRGLHGRLRRELAVEFEGERLAYRPFGPDYAPPHL
                     EWGLNWGVAFHAGRLLMLHAAVVARGERALLLPAQPGSGKSTLAAALGFRGWRFLSDE
                     FCLIDLQRFELLPFPRLVALKNASVPAMRAHAPAAVIGRDFPNTAKGCLAHLQPPPGS
                     IEAMDRPAVPAWIVFPQFRAGSRARLEAVPPERTMARLCTNAFNYEHTGLTGFETVAR
                     LVSGCPARALEFGTLDDAIAAIDALADGREPLSAAGAR"
     gene            complement(5346..5885)
                     /locus_tag="PK144_11915"
     CDS             complement(5346..5885)
                     /locus_tag="PK144_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66482.1"
                     /translation="MSSSTPLLACLLLATAGSAFAASFDCTRAGTLVEQAICSNPELS
                     DLDDAMSNAYREALAQAADAAVVQATQRRWLSEVRNPCRNTGCLRSAYRTRLRELAAV
                     SPAVQPARELRIVGRVRYGTLDSAIETESGRSYGFGSDSAIGARILDTCGDRGVCEVS
                     GFVDADDTLTRVLSVRRLR"
     gene            6049..7011
                     /gene="accA"
                     /locus_tag="PK144_11920"
     CDS             6049..7011
                     /gene="accA"
                     /locus_tag="PK144_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNF66483.1"
                     /translation="MNMNFLDFEQPIAELEAKLQELRKLCNDTELNISDEMERLETKS
                     RELTESIFKTLTPWQVSQIARHPQRPYTLDYVEHLFTGFEELHGDRAYADDPAIVGGL
                     ARFDGRPVMVIGHQKGRDTREKLRRNFGMPRPEGYRKALRLMKLAERFRLPIITFIDT
                     PGAYPGIGAEERGQSEAIARNLFVMAQLRVPVLCTVVGEGGSGGALAIGVGDKVNMLQ
                     YGTYSVISPEGCASILWKSADKAPDAAQAMGITAPDLQKLGLIDEIIPEPLGAAHRDP
                     AQTAAAIRTALLAGLAELDGLDLDALLARRHERLMAYGEFTVRE"
     gene            7008..>7790
                     /gene="tilS"
                     /locus_tag="PK144_11925"
     CDS             7008..>7790
                     /gene="tilS"
                     /locus_tag="PK144_11925"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNF66484.1"
                     /translation="MNDTACATAAGTVERTLVAALSRLCAAWPQTRRLTVGFSGGLDS
                     TVLLHVLAHLRPAGLQLDALHVDHGWRPESADWARHCIARATALGVGCRVLRIEARAA
                     PGESPEAAARTARYAALGARLDAGNALLTAQHADDQAETVLLMLLRGAGPKGLAAMPE
                     DAALGSGRLWRPLLGVSRAALQAYATHYRLDWLDDPANTDTDYDRVFLRERLMPVLAG
                     RFPAAARTLARSARLSAEAQAAVETLAAQDLDGLTGGGAGPAG"
BASE COUNT         1124 a         2739 c         2818 g         1109 t
ORIGIN      
        1 cgcttgcccg gaacgaagcc gcccgccgct cgtggggcgg gcggtgcggc gatgccggcg
       61 ggcgcggttc aggcgtccgg ggccggaggg gacttggctt cgggggcctg cacgacggca
      121 tcggcttcga tctgccggtc cttcgcgcgc cggcgcagcc gcatgaacag ccacgcgagc
      181 accgccagcc cgatgatcgt gccgaggatg tacagctgcg cgttgtgcat gcggccgagg
      241 aaggtttcca tcgcctcgcc gaacaggtag ccggcgccgg cgaagctgat cgcccagacc
      301 gcggcgccga tcatgttcag cacgatgaag cgcaccgtgg agatgtcgct cgcgccgatc
      361 acgaacggcg tgatgttgcg cacgccgtaa tagaaccgga aggtcagcac caggaagtcg
      421 cggtggcgtt cgaggtggcg gttgatccag gccacgcgcc cggcccactt cggatgcttg
      481 tcgagcagct tgcggccctg cttgcggccg atgaagaagt agaactggtc gccgcagagg
      541 ctgccgacga aggccgacag catcaccagc ggcagttcga ggtgtccgag cttggcgatg
      601 acgccggcga cgatgacgac ggtttctcct tcgatgaacg tccagatcag aatcgtcaga
      661 tagccgtatt cggctatcca gtgctggatg ttttcaaaca tgcgcacgca ctcgggctgt
      721 cggtaggagc aggggcggcg gaccgcgcgg gggcgccatt gtgtcggagc ctccgtgacg
      781 cgtccatgac gcggcgccgc agtgcggtcc tgcgctgcgg cgccgtgctg cggctcactc
      841 gggcggcgtc agtgccagga acagcgggga ttcgccgcgc ctgaccagca ccggcaccgg
      901 cttgcccttg ccgagctttg cggcgagttc ctcgaactgc gcgactccgg tgaccggctg
      961 gttgtcgagg cgcacgatca cgtcgccggt gcggatgccg gcgcgagcgg ccggtcccgg
     1021 ggcgacctcg cgcacgacca cgccggtctc gcccttgcgc gcggcctcgg gcggcagctc
     1081 gccgagcgtc aggccgaggc cgcgccgggt gccgctgccg gccttgccgg cctgacgcgc
     1141 atcgtcgggc agttcctcga tcttcacggt gatcgtggtt tcccggccgt cgcggaacac
     1201 cgacagcgtc gcgtccttgc ccggcaggta gctgccgacc agcggcggca gctcgctgga
     1261 gcgcagcagc ggccggccgt tgaacgcgag cacgatgtcg ccgggcttca ggccggcctt
     1321 cagcgccggg ccttcgggca gcacctggcc gatcagcgca ccgcgcgccc ggtcgaggcc
     1381 gaaggacttc gccagatccg gcgtgacctc ctggatcagc acgccgagcc agccgcgcgc
     1441 gacgcggccc ttggtcttga gctgttcggc gacatccatc gcgacgttga tcgggatcgc
     1501 gaacgacagg ccctgatagc cgccggagcg cgagtagatc tgcgagttga tgccgaccac
     1561 ctcgccgttg aagttgaaca gcgggccgcc ggagttgccg gggttgaccg cggcgtcggt
     1621 ctggatgaac ggcacgtagg agtcactcgg caggctgcgc ccgagcgcgc tgacgatgcc
     1681 ggcggtcgcg ctcgattcga agccgaacgg cgagccgatc gccagcaccc actggccgac
     1741 cttgagctgt tcggcatcgc cgaggcgcac gaccggcagc ggcgcggcac cgtcgatctt
     1801 cagcagcgcc acgtcgctgc gcttgtcggc accgatcacc ttcgcgatca gctcggtgcg
     1861 gtcgcgcaac ccgaccgtca ccgtgtcggc gccctcgacg acgtgggcgt tggtcagcac
     1921 gtagccgtcg gccgagatca cgaagcccga gccgatcgag gccggcagct cgcgcgggcg
     1981 cggcgaattg ccatgcggca tctgctcgaa gaagcgccgg aacggctcct cgaacggcgt
     2041 gccctcgaac ggattctgga ccggcggctt gccgggtttg tcctcgtcgg gctcgtcggc
     2101 ggcgtccttc gcggcggtgc gggcgctgac gctgaccacg gccggcgcgt actgctcgac
     2161 cagcgtcgtg aagtcgggca gctcgacggc cggcgcgcgg ccggcggcga gcagcacgag
     2221 gcaggtcagc agcacccgca ggagcggatt cgatccgggc atctgggcct ccgtagccgt
     2281 cagtaggcag aaatcatcag ggttgtccgg ggggcggccg gggcggttcg gccggggtgg
     2341 cgacctcgac ggccgccgcc ggtgcaccgg gcgcggccac gccgaggcgc gtgcgcagcc
     2401 gctgcacggc cttgcgggcg aggtgcggca gcagcaggcg tggcggcatg cgcagccagt
     2461 gcgaacgaat gtagagcgcc agcgccgcca gaggtgccat cggtcgttca tgcagcaccg
     2521 catgcaccag cgccggcagc acgccggcca gctgcggcgg cggggcgtgt gcggccagcg
     2581 ccgtgaacac ggccgccggc accggcgtgc cgagttcgcg ctgcaccagc gtcagcgcgt
     2641 accagagcgg ccgcgccaga tcgagcgcca cggcccgcgc cggcagcgcc tgccagaagg
     2701 tctcgtcgcc gagatcggtg atcagcccgt gcaggtcgag cagatcgcgc agcggctgcg
     2761 cgaaggcgcc gtcgtaacag gcatgcacgg ccgcgtgcag cagcaggtcg ctcgcgcacg
     2821 gtacatataa atccggcacg cccggcaccg gctcggccgc tgccagcagc agggccggat
     2881 cgggatgcag gcgcgcggtc gggggcagga tgccgtggtg cagatcgagc tgcgtgccgc
     2941 ggtgcgcgtg gtggaaggcc ggcagttcgt gcatccaggc gcggtagtag gcgtcgtcgt
     3001 aatcgctgtg cgcggcgtgc tcccagccgc cggcgagcag cccggcctcg gcttcggcga
     3061 tccgcgcctt cggcaccagc acgtcgagat cggctgacag ccgtccctgc accgagggca
     3121 gcccgcgcag cgcacaggcc acgcccttga gcaccagcac cggcacgccg ggcagcacga
     3181 ggcgcaggtt cagcgcctcc cagcgcagcc ggcgcatccg cgactgcgcg cgcacccgcg
     3241 cgctgtcgag atggaagcgc acgcgcgccg gcacgcgatc gagcacgccc cgcgccgcga
     3301 gcaccgtggc cagccgcccg agcgcctcgt gccggcgcgc gtacggcagc agttgctccc
     3361 agtcggcgcc ctgcaggtga gccggatcgg gaatcgaacc gcagaacagc gccagcagca
     3421 gggtggtcgg gcggcgctcg ggcggcgggc gggacgtggg cggcatcggg gacaggtggg
     3481 tggcagttgg gaaattttta cagtgcccgg ccggcagtgg caggggtgaa gcagacctgg
     3541 ttttgaattc agtgacttgg ggttttgtgt ctgattggct tgaaatctgc ataaagccgc
     3601 acgagacaca gggaaactcg gctgcaagcc ttgcggatgg cccggtggag tggcatccgg
     3661 gtgccgattc gtcagactat aaggcaaggc cttttgagga cagcaagcga tgcaagacca
     3721 cgaacactcc ggcacggatg gtcaggacgc cgcagccccg gtatccctgc gccgccgccg
     3781 gctgctgaag tcggctgccg tcgtggccgt tcccgcggtg ctgaccctgc acgccggcta
     3841 tgcgcgtgcc gcgaacagca gcgccgtgcg ctgcgcgctc aacaccgtca acggcaccgg
     3901 catccggatc accaacgaga acacccccgg cctgtacaag gaaatggggg cggacagcca
     3961 gctgcacgcg gtctggttcc agatcgatcc gtccagcggc ggcatcacgc cggtccacta
     4021 caacgagacc ggttacaagg tcggcaccaa cgcctcgttc ctgtcgagcg gctgcgtgaa
     4081 ctccgcgctc ggcatctgag cccgtgcacg gcactcccga accccgccat ccggccgcct
     4141 tctggcggcc gaatgctttt tcgcgcctgc actggcaggc gagcgacgag gccggcgaat
     4201 tccacctcgt ccacgacgag acctcgggcc gcacgcactt cctgacgccg ctcggtgcct
     4261 ggctgctgca gcggctcgcg gaggcgccgg ccggctgcgc gcagctgcag ctcgaactgc
     4321 gcgccgactg cgacgatccg gatgcgcccg gcctcgacca ggcgctcgcc gatgcgctcg
     4381 acctgctcgc tgccgaactg ctgatcgaac cctgtccgcc gtcatggccg cctgcctcgg
     4441 cgagctgagc cgcgccgaac tccgggcgcg gctggccgac aatgcgctgc gcctgcgcat
     4501 cggcccgttc gcgtacgcgc tcggctccga atggccgggg ctggccgact ggctggcgcc
     4561 gctctatgcg cgctatccgc ggtttccgga cgatgaatgc gcggatttcc gcgtgcgcct
     4621 gcgctggcgc gggctgcacg ggcggctgcg gcgcgagctg gcggtcgagt tcgagggcga
     4681 gcggctggcc taccggccgt tcggtcccga ctacgcgccg ccgcacctcg aatgggggct
     4741 gaactggggg gtggccttcc acgccggccg gctgctgatg ctgcacgcgg cggtggtcgc
     4801 gcgcggtgag cgcgcgctgc tgctgccggc ccagcccggc agcggcaaga gcacgctcgc
     4861 ggccgcgctc ggattccgcg gctggcggtt tctgtccgac gagttctgcc tgatcgacct
     4921 gcagcgcttc gagctgctgc cgttcccgcg gctggtggcg ctgaagaatg cctcggtgcc
     4981 ggcgatgcgc gcgcacgcgc cggcggccgt gatcggccgg gattttccga acaccgccaa
     5041 gggctgtctc gcgcacctgc agccgccgcc cggcagcatc gaggccatgg accgcccggc
     5101 cgtgccggcg tggattgttt tcccgcagtt ccgggccggc agccgcgcac ggctcgaagc
     5161 cgtgccgccg gagcgcacga tggcccgtct ctgcacgaat gccttcaact acgagcacac
     5221 cggcctcacc ggcttcgaga ccgtggcgcg gctggtgagc ggctgtccgg cgcgggccct
     5281 cgaattcggc acgctcgatg acgccatcgc cgccatcgac gcgctcgcgg atggccggga
     5341 gccgctcagc gcagccggcg cacgctgagc acgcgcgtca gcgtgtcgtc ggcatcgacg
     5401 aagccgctga cctcgcagac gccgcgatcc ccgcaggtat cgaggatgcg cgcgccgatc
     5461 gccgagtcgc tgccgaagcc gtagctgcgc ccgctttcgg tctcgatcgc cgaatcgagc
     5521 gtgccgtagc gcacgcgccc gacgatgcgc agctcccgcg ccggctgcac cgccggcgac
     5581 accgcggcga gttcgcgcag gcgcgtgcgg taggcgctgc gcaggcagcc ggtgttgcgg
     5641 cagggattgc gcacctcgct cagccagcgc cgctgcgtgg cctgcaccac ggccgcatcg
     5701 gcggcctgcg ccagcgcctc gcggtaggcg ttgctcatcg cgtcgtcgag atcggagagt
     5761 tccgggttgc tgcagatcgc ctgttcgacc agcgtgccgg cgcgcgtgca gtcgaagctc
     5821 gcggcaaagg cgctgccggc ggtggcaagc agcaggcagg cgagcagcgg ggtggaggag
     5881 gacatgatcg gcggttcctg cgcgggtggt ggggacgtgc gccagcgtag ccgccgccgg
     5941 acccgccggg gccaccgcgc ccgcgcccgg ccgcctgcct tgccgcttcg gcattcaccg
     6001 gtacactacg cggcttccct gcgccgccgc ggacgccgac gcctcatcat gaacatgaat
     6061 ttcctcgact tcgagcaacc gatcgccgag ctggaagcga agctgcagga gttgcgcaag
     6121 ctctgcaacg acaccgagct gaacatctcg gacgaaatgg aacggctcga aaccaagagc
     6181 cgcgaactga ccgagagcat cttcaagacg ctgacgccgt ggcaggtctc gcagatcgcc
     6241 cggcatccgc agcgtccgta cacgctcgac tacgtcgaac acctgttcac cggcttcgag
     6301 gagctgcacg gcgatcgcgc ctatgccgat gatccggcca tcgtcggcgg actggcccgg
     6361 ttcgacggtc gtccggtcat ggtcatcggc catcagaagg gccgcgacac cagggaaaaa
     6421 ctgcgccgca acttcggcat gccgcggccc gagggctacc gcaaggcgct gcggctgatg
     6481 aagctcgccg agcgcttccg gctgccaatc atcaccttca tcgacacccc cggtgcctat
     6541 ccgggcatcg gtgccgagga gcgcggccag tccgaggcca tcgcccgcaa cctgttcgtg
     6601 atggcgcagc tgcgcgtgcc ggtgctctgc accgtggtcg gcgagggcgg ctcgggcggg
     6661 gccctggcga tcggcgtcgg tgacaaggtc aacatgctgc agtacggcac ctactcggtg
     6721 atctcgcccg agggctgcgc gtcgatcctg tggaagagcg ccgacaaggc accggacgcc
     6781 gcgcaggcga tgggcatcac cgcccccgat ttgcagaagc tcggcctgat cgacgagatc
     6841 atccccgagc cgctcggggc cgcgcaccgc gatccggcgc agaccgccgc cgcgatcagg
     6901 acggcgctgc tcgccggact cgccgaactc gacgggctcg acctcgatgc gctgcttgcg
     6961 cgccggcacg agcggctgat ggcctacggg gaattcacgg tccgcgagtg aatgacactg
     7021 cctgcgccac cgcggccggg accgtcgagc gcacgctggt cgcggcgctg tcgcggctct
     7081 gtgcggcctg gccgcagacc cgccggctga cggtcggctt cagcggcggg ctcgattcga
     7141 cggtgctgct gcacgtgctg gcgcacctgc gcccggccgg gctgcagctc gatgcgctgc
     7201 acgtcgatca cggctggcgg ccggagtccg ccgactgggc gcgccactgc atcgcgcgcg
     7261 cgacggcgct cggtgtcggc tgccgggtgc tgcgcatcga ggcccgtgcc gcgcccggcg
     7321 aatcccccga ggctgcggcg cgcacggcgc gctacgcggc gctcggcgcg cgcctcgatg
     7381 ccggcaatgc gctgctcacc gcccagcatg ccgatgacca ggccgaaacc gtgctgctga
     7441 tgctgctgcg cggggccggc ccgaaggggc tcgcggcaat gccggaagac gcagccctcg
     7501 ggagcgggcg gctgtggcgg ccgctgctcg gcgtgtcgcg ggccgcgctg caggcctacg
     7561 cgacgcatta ccgcctcgac tggctcgacg acccggccaa caccgatacg gactacgacc
     7621 gcgtgttcct gcgggagcgg ctgatgccgg tgctcgccgg gcgcttcccg gctgcggccc
     7681 gcacgctggc gcgcagcgcc cggctcagcg ccgaggcgca ggccgcggtc gagacgctgg
     7741 ccgcgcagga tctcgatggc ctgaccggcg gcggcgccgg gcctgccggg
//
LOCUS       DAOQIE010000187         7781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4870_length_7781_cov_6.804038, whole genome shotgun sequence.
ACCESSION   DAOQIE010000187 DAOQIE010000000
VERSION     DAOQIE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4870_length_7781_cov_6.804038"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..730
                     /locus_tag="PK144_11930"
     CDS             248..730
                     /locus_tag="PK144_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNF66485.1"
                     /translation="MIEALQLLFLLFVANGAPILATRVLDTAGDHPIDGGHRFLDGRP
                     LFGPSKTWRGLGVALLASTLAALLLGWPAWIGLLIGAAAMLGDLLSSFLKRRLGLASS
                     AQALGIDQVPEALLPLLAVRETLALDGWVIAALVAAFVVLELLLSRLLYRLHIRRQPY
                     "
     gene            complement(743..1768)
                     /locus_tag="PK144_11935"
     CDS             complement(743..1768)
                     /locus_tag="PK144_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF66486.1"
                     /translation="MPDSAILAGTGERRGAAVPMDPPAAMPPVRPDPELAALAARLSP
                     VHLRQRLNMEGAHGKAVFGYGRTFFHLENWYSIHALIRGVLKLGGLYGRGLRNALAIA
                     VTRHEARLPALPAAFDGLRVLQLSDLHLDASEAFVHALAERVRGVEADLTVLTGDFRY
                     RTHGDWRPALERLERLRPQLPGEVYAILGNHDTVRMVPRIESLGIRLLMNEHVTLTRA
                     DARLHLAGIDDPHFFEAGNIERAADGIPAEDCAILLSHSPETWWQAAHAGFDLLLAGH
                     THGGQVCLPGGLPLMLNADCPRAYCRGPWRYRDMLGYTSTGAGSSIVGVRYNCPPEIV
                     VHTLRRG"
     gene            complement(1970..3757)
                     /locus_tag="PK144_11940"
     CDS             complement(1970..3757)
                     /locus_tag="PK144_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNF66487.1"
                     /translation="MKASTAPRPQAGRRLGLEEVLRLLVEDGEISEASAAELRKQRTR
                     SANSAHPLVFIGEQRLPSAQQPKRVLSTEMLTEWLAGRTGFPYFHIDPLKIDVSKVCG
                     VVSHAYATRFHILPVAVSDTAVTIATAEPFDREWQSVLEQGLRLNIRPVIANPADIVR
                     YLAEFYSLARSVQSAMKSPQSLPSAITNFEQLVKLGGKHGVDANDRAIVHIVDWLLQY
                     AFEQRASDIHIEPRRDECLVRFRIDGALHLVYQGPMTVMVAAINRIKLLARMDVVEKR
                     RPQDGRIKTGTPNGEEIELRLSSMPTAFGEKLVLRIFDPNVLLKSRAELGFSDEENRL
                     WSGLVHRPHGIVLVTGPTGSGKTTTLYSTLRELATPEVNVCTIEDPIEMVYPYLNQMQ
                     VQPNLGIDFALGVRTLMRQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNDAP
                     AAVVRLMDLGVPPYLIRSTVLGVVAQRLVRTVCPHCAEPEPVDAAAWEALVRPWSVAM
                     PAQSMRGAGCLECRMTGFRGRTGLYEMLMLSPELALHVDSPLDLDAMRAQAIREGMRP
                     LRIAGALKVAAGLTTIDEVARNTPPVGRG"
     gene            complement(3995..4765)
                     /locus_tag="PK144_11945"
     CDS             complement(3995..4765)
                     /locus_tag="PK144_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HNF66488.1"
                     /translation="MTTDSQHPRKAELYRMVMKEHLCPFGLKSLDLLKREGYAVDDHW
                     LRTHQETDAFMARHGVETTPQTFIDGQRIGGYDDLRRFFGKPLRDPDAVTYQPVIAVF
                     GMAALMALAACQAAFGTVLTRQAGEWFIAFSMCLLAMLKLRDLESFSNMFLGYDLLAQ
                     RRVRYAYLYPFGEALAGVLMIAGGAGLWLAVPVAFVIGTIGAISVFKAVYIDRRELRC
                     ACVGGDSHVPLGFVSLTEDLMMVAMAVWMGLKYLWPLL"
     gene            5050..5580
                     /locus_tag="PK144_11950"
     CDS             5050..5580
                     /locus_tag="PK144_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNF66489.1"
                     /translation="MRTYIRTRNKGGCYFFTVNLAERPGDGLLVRHVDHLRSAFRETR
                     ADHPFMIDAIVILPDHLHCIWQLPPDDDDYPTRWRLIKSGFSRRVVTNERVSESRQRK
                     HERGLWQRRYWEHAIRDDEDYRQHLDYIHYNPVKHGLVESPKDWDYSSFRRFVDQGMY
                     SLEWAANMEIDRLHLE"
     gene            5767..7605
                     /locus_tag="PK144_11955"
     CDS             5767..7605
                     /locus_tag="PK144_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HNF66490.1"
                     /translation="MDTLVFVLIAVATIVVMLIAAAFAYRHAARLQRTLADAQQRAQE
                     AEATLQGAITENLARGRRIDDLSTELAEYELRLQRANADLARYRTISNAEAEAARLLQ
                     TAREAIAQERAELIASRQRAEAEAERIRTQAAQTREHAQADASALLERSRQEAMAQTE
                     AIQTEALLARKEAQALRARLREDAKNLQARTDAEIARVTAQAQRLIDEAQEKAAAIAG
                     EALRARDKADLFERTVTAMKNIIEGYGERYFVPTHTLLDDVADVFGHTEAGQELKTAR
                     ALSAAMVRGGQAATCDYVEAQRRQTAINFVVDAFNGKADAILARLRHDNVGKLEQELR
                     DAFTLVNFHGAAFRDARILPSYLDARLAELRWGAIAYELQLREREEQRLIKERMREEE
                     KARREYERAIREAAKEEEQLQRALARARREIEQATGEQRARYEAQLAELAAKLQIAEE
                     KNQRALSMAQQTRAGHVYVISNIGSFGENVLKIGMTRRLEPLDRVRELGDASVPFSFD
                     VHALVYSEDAPGLERELHRRFNALRLNKVNFRKEFFRLELAHVRQTMTDLGLDPHFTL
                     TAEAREYRESIAVDHLPAEHRAAVLDSLLELEETADADAANAEEEA"
BASE COUNT         1364 a         2554 c         2632 g         1231 t
ORIGIN      
        1 actgagctat tcccgcatga atcgttgtgg agcgtccctt ccggggtcgt ccaggggtcg
       61 tccaggtgtc gtcggtgacg acggcgcgca tcttacggag ccactcccgg gcaggcaagc
      121 cccggatgaa ggtttcgttg catttatttg aacgatggca agcgcacgac tggcatcggc
      181 agcgaagctc gcggacactc gcattccggc gcatcgcccg accgtccccg tgtgcaccgg
      241 agcctgcgtg atcgaagccc tgcagctgct gttcctgctg ttcgtcgcca acggcgcgcc
      301 gatcctggcg acgcgcgtgc tcgataccgc cggcgatcac ccgatcgacg gcggccaccg
      361 cttcctcgac ggccggccgc tgttcggtcc gagcaagacc tggcgcggcc tcggcgtggc
      421 gctgctggcc agcacgctcg ctgcgctgct gctcggctgg ccggcgtgga tcggtctgct
      481 gatcggcgca gcggccatgc tcggcgatct cctttcaagt tttctgaagc gccggctcgg
      541 ccttgcatcg agcgcgcagg ctctcggcat cgatcaggtc ccggaggccc tgctgccgct
      601 gctggcggtg cgcgagaccc tggcgctcga tggctgggtc atcgccgcgc tggtggcggc
      661 gttcgtggtg ctggaactgc tgctgtcgcg cctgctgtac cggctgcaca tccgccgcca
      721 gccctactga agcgcagcgg cctcagcccc ggcgcagcgt gtgcacgacg atctccggcg
      781 ggcagttgta gcgcacgccg acgatcgacg aaccggcccc ggtcgaggtg tagccgagca
      841 tgtcgcggta gcgccacgga ccgcggcagt aggcgcgcgg gcagtcggcg ttcagcatca
      901 gcggcaggcc gccgggcagg cagacctggc cgccgtgcgt gtggccggcg agcagcaggt
      961 cgaagccggc gtgcgcggcc tgccaccagg tttccggcga gtgcgacagc aggatcgcgc
     1021 agtcctcggc cgggatgccg tcggcggcgc gctcgatgtt gccggcctcg aagaagtgcg
     1081 gatcatcgat gccggcgagg tgcaggcgcg cgtcggcgcg cgtcagcgtc acgtgctcgt
     1141 tcatcagcag ccggatgccg agcgactcga tgcgcggcac catgcgcacg gtgtcgtggt
     1201 tgccgaggat cgcgtagacc tcgcccggca gctgcggacg caggcgttcg agccgttcga
     1261 gcgccggacg ccagtcgccg tgcgtgcggt agcggaaatc cccggtgagg acggtcaggt
     1321 cggcctcgac gccgcgcacg cgctcggcca gcgcgtgcac gaaggcctcg gaggcatcga
     1381 ggtgcaggtc ggacagctgc agcacgcgca ggccgtcgaa ggccgccggc agcgccggca
     1441 gccgcgcctc gtgccgggtc acggcgatcg cgagcgcgtt gcgcagcccc cggccgtaga
     1501 ggccgccgag cttcagcacg ccgcggatca gcgcgtggat cgaataccag ttttcgagat
     1561 ggaagaaggt gcggccgtag ccgaacacgg ccttgccgtg cgcgccctcc atgttcaggc
     1621 gctggcgcag atgcaccggg ctcaggcggg ccgcgagtgc ggcgagttcg ggatcgggcc
     1681 ggacgggggg catggcagca ggggggtcca tcggtacggc agcgccgcgc cgttcgccgg
     1741 tgccggcgag aatcgcggaa tcaggcatgt tcggtcactc cccggtttgt tgccccttcc
     1801 ctggagagag gctttgcagt gcgcgcggcc ccgcacgcgt gacgttcatt cgccgtccgc
     1861 atttaagcag ccgccaccgg catgcggcgg cgcaccaatg atgaaaagtt gataagggcc
     1921 ttgtatcgca gcgatccgtt gccagcgcgc aacagcgccg ccggcgacct cagcccctgc
     1981 cgaccggcgg cgtgttgcgg gcgacctcgt cgatcgtcgt caggccggcg gcgaccttca
     2041 gcgcaccggc gatgcgcagc ggccgcatgc cctcgcggat cgcctgcgcg cgcatcgcgt
     2101 ccagatcgag cggcgaatcg acgtgcaggg ccagctccgg actcagcatc agcatctcgt
     2161 acaggccggt gcggccgcga aagccggtca tccggcattc gaggcagccg gcaccgcgca
     2221 tggactgcgc cggcatcgcc accgaccacg gccgcaccag ggcttcccag gcggcggcat
     2281 cgaccggctc gggttcggcg cagtgcggac agacggtgcg caccagccgc tgcgcgacca
     2341 cgccgagcac ggtcgagcgg atcaggtagg gcggcacgcc gaggtccatc aggcgcacga
     2401 ccgccgcggg cgcatcgttg gtgtgcagcg tcgacagcac cagatgcccg gtcagcgccg
     2461 cctgcaccgc catctcggcg gtttcgaggt cgcggatctc gccgaccatg atcacgtccg
     2521 ggtcctgacg catcagcgtg cgcacgccga gcgcgaagtc gatgccgaga ttcggctgta
     2581 cctgcatctg attcagatac gggtacacca tctcgatcgg gtcctcgatc gtgcagacgt
     2641 tgacctccgg cgtcgccagc tcgcgcagcg tcgagtacag cgtcgtggtc ttgcccgagc
     2701 cggtcgggcc ggtgaccagc acgatgccgt gcggccggtg cacgagtccg ctccagaggc
     2761 ggttctcctc gtccgagaag ccgagttcgg cgcggctctt cagcagcacg ttcggatcga
     2821 agatgcgcag caccagcttc tcgccgaagg cggtcggcat cgacgacagg cgcagctcga
     2881 tctcctcgcc gttcggcgtg ccggtcttga tgcgcccgtc ctgcggccgg cgcttctcga
     2941 ccacgtccat gcgcgcgagc agcttgatgc ggttgatcgc cgcgaccatc accgtcatcg
     3001 gcccctgata gacgaggtgc agcgcgccgt cgatgcgaaa gcgcaccagg cattcgtcac
     3061 ggcgcggctc gatgtggatg tcgctggcgc gctgctcgaa ggcgtactgc agcagccagt
     3121 cgacgatgtg gacgatggcg cggtcgttgg catcgacgcc gtgcttgccg ccgagcttga
     3181 ccagctgctc gaagttggtg atcgcgctcg gcagcgactg cggcgatttc atcgccgact
     3241 gcaccgagcg cgcgaggctg tagaactcgg cgagatagcg gacgatgtcg gccggattgg
     3301 cgatcaccgg ccggatgttc aggcgcagcc cctgttcgag cacgctctgc cattcgcggt
     3361 cgaagggctc ggcggtggcg atggtgacgg cggtgtcgct gactgcgacc ggcaggatgt
     3421 ggaagcgcgt ggcgtaggcg tgcgagacga cgccgcagac cttggacacg tcgatcttca
     3481 gcgggtcgat gtggaagtac ggaaagccgg tgcgcccggc gagccattcg gtcagcatct
     3541 cggtgctgag cacgcgcttc ggctgctgcg cgctcggcag gcgctgctcg ccgatgaaca
     3601 ccagcggatg cgcgctgttc gcgctgcgcg tgcgctgctt gcgcagttcg gccgcactcg
     3661 cctcgctgat ctcgccgtcc tcgaccagca gccgcagaac ctcttcgagc ccgagcctgc
     3721 gcccggcctg cggccgcggc gccgtggatg ccttcatgat cactgcctcg ctgtcgctgc
     3781 cggtgccgca gcggcggaag cgccggcacc gtccccgcac cggcctgcgg cgcggggtcg
     3841 gacaccttag aggatttcgg ccggtggtgg catgggtggt ccggtcaaga aatgcgaaac
     3901 cgccggaccc ggacgggcag cacggcaggc agccggagat gcagcaggcc aacgcgggcg
     3961 gacttgtgcg gacagaggcc cgcgcccgcc ggctttacag caagggccac agatacttca
     4021 gccccatcca caccgccatc gccaccatca tcagatcctc ggtcagcgac acgaagccga
     4081 gcggcacgtg gctgtcgccg ccgacgcagg cgcatctgag ttcgcgccgg tcgatataga
     4141 cggccttgaa caccgagatc gcgccgatcg tgccgatcac gaacgcgacc ggcacggcca
     4201 gccagagtcc ggcgccgccg gcgatcatca gcacgccggc gagcgcctcg ccgaacggat
     4261 agaggtacgc ataccgcacc cggcgctgcg cgagcaggtc gtagccgagg aacatgttcg
     4321 agaagctttc gaggtcgcgc agcttcagca tcgcgagcag gcacatcgag aaggcgatga
     4381 accattcccc ggcctgccgc gtgagcacgg tgccgaacgc ggcctggcag gcggccagcg
     4441 ccatcagcgc cgccatcccg aacaccgcga tgaccggctg gtaggtgacg gcgtccggat
     4501 cgcggagggg tttgccgaag aagcggcgca ggtcgtcgta gccgccgatg cgctggccgt
     4561 cgatgaaggt ctgcggcgtc gtctcgacgc cgtgccgggc catgaaggcg tcggtttcct
     4621 gatgcgtcct cagccagtgg tcgtcgacgg catagccttc gcgcttcagc agatcgaggg
     4681 acttgaggcc gaaggggcag aggtgctcct tcatgaccat gcggtacagc tcggccttgc
     4741 gggggtgttg agagtcggtc gtcatcgcgg gctccggtcg gggggaacgg atatcggacg
     4801 agccggggat gcgcaggccc gtccaaagat gaccgcggtg cgatgcaggc gttgcacgcg
     4861 atagggggag tgccagagcc gggcggatcg ggatgcgggg agcttgtctg gcgcggccgg
     4921 cggtggtctg gtggaagggc agcggaaaat ggaagcggca gcgcgtgatt ctgcgccggc
     4981 aggctgggtt gaggaacgaa acccagcact ccagactgca cggaaccgtc tgccggctgc
     5041 gacaggaaca tgcgcaccta catccggact cgaaacaagg gtggctgcta ttttttcacg
     5101 gtgaatctcg ccgagcggcc cggagacggt ctgctggttc gccacgtcga tcacctgcga
     5161 tcggcattcc gggaaacgcg agccgatcat ccattcatga tcgatgcgat cgtgatcctg
     5221 ccggatcatc tgcattgcat ctggcaactg cccccagacg atgatgatta tccgacccga
     5281 tggcggctga tcaaatcagg attttcgcgg cgagtcgtca caaacgaacg ggtatcggaa
     5341 tcacggcagc gaaaacatga acgcggattg tggcagaggc gctattggga acatgcgatt
     5401 cgtgatgatg aggattaccg gcagcatctg gattatattc attacaatcc ggtcaagcat
     5461 ggacttgtcg aatctccgaa ggattgggat tattcatctt ttcggcggtt cgtagatcag
     5521 ggcatgtatt cgcttgaatg ggcggcgaat atggagatcg atcgtttgca tctggagtga
     5581 gcttgatgct gggtttcgtt cctcaaccca gcctacacgc tatctgccag cgacgagatc
     5641 accgtgctcg gcatcctgac tcgccatctt gcgcaggcgg tgcagaactg acctcgcgca
     5701 agccattacc ggtgcacctc cgctaccatc gccgcatcga tctggctgca caaggagtac
     5761 agggacgtgg atacgctcgt gttcgttctc atcgccgtgg cgacgatcgt cgtcatgctc
     5821 atcgccgccg cctttgccta ccgccatgct gcgaggttgc agcgaacgct cgccgatgcc
     5881 caacagcgag cgcaggaagc cgaagcgacc ctgcaaggag cgatcaccga aaacctggcg
     5941 cgcgggcggc gaatcgacga tctgtccacg gaactggccg agtacgaact gcggctgcag
     6001 cgcgctaatg ccgatctggc gcgttaccgc acgatcagca atgccgaggc cgaagccgca
     6061 cgcctgctgc agaccgcccg cgaagccatc gcccaggaac gtgccgaact cattgccagc
     6121 cgtcagcggg ccgaggcgga ggccgaacgc atacggacgc aggcggcgca aactcgcgag
     6181 cacgcacagg ctgatgcttc agcattgctc gaacgcagcc ggcaggaagc catggctcaa
     6241 accgaggcga tccaaaccga ggccctgctg gcccggaagg aagcacaggc gttgcgtgcc
     6301 cggctgcgcg aagacgccaa aaatctgcaa gcccgcaccg acgccgaaat cgcccgcgta
     6361 accgcccaag cgcaacgtct gatcgacgaa gcccaggaaa aagccgccgc gatcgctggc
     6421 gaagcgcttc gcgcgcgcga caaggccgat ctgttcgagc gcaccgtcac ggccatgaag
     6481 aacatcatcg aaggctacgg cgagcgctat ttcgtgccca ctcacaccct gctcgacgat
     6541 gtcgccgatg tcttcggtca cactgaagcc gggcaggaac tcaagacggc gcgggcgctg
     6601 tcggccgcca tggtgcgcgg cggtcaggcg gccacctgcg attacgtgga agcccagcgc
     6661 cggcaaaccg ccatcaactt cgtcgtcgat gccttcaacg gcaaggcgga cgccatcctg
     6721 gcgcggctgc gtcacgacaa cgtcggcaag ctcgaacagg agctgcgtga cgcattcacc
     6781 ttggtcaact tccacggcgc tgccttccgc gatgcccgca tcctgcccag ctacctcgat
     6841 gcacggctgg ccgagctgcg ctggggcgcg atagcctacg aactgcaact gcgcgagcgc
     6901 gaggaacagc gcctgatcaa ggaacgcatg cgcgaggagg agaaagcccg gcgggagtac
     6961 gagcgggcca tccgcgaagc ggccaaggaa gaagagcagt tgcagcgcgc tcttgcccgc
     7021 gcccgacggg aaatcgagca ggctaccggt gaacagcggg cgcggtacga ggcgcaactg
     7081 gccgaactcg cggcaaagct gcagatcgcg gaggagaaga atcagcgagc cttgtcgatg
     7141 gcgcagcaaa cccgcgccgg ccatgtttac gtcatctcga acatcggctc gttcggtgag
     7201 aatgtcctca agatcggcat gacgcgacgc ctggagcccc tcgaccgcgt gcgcgaactg
     7261 ggtgacgcct cggtgccctt ctccttcgat gtgcacgcac tggtatacag cgaggacgca
     7321 ccggggctgg agcgggaact gcatcgccgt ttcaatgccc tgcggctgaa caaggtcaac
     7381 ttccgcaagg agttcttccg gctggaactc gcacacgtcc gccagaccat gaccgatcta
     7441 gggctggatc ctcatttcac tctgaccgcc gaggcgcgcg agtaccggga atcgatcgcc
     7501 gtcgatcacc tgccggccga acatcgtgca gcagtgctgg actcgctgct tgaactcgaa
     7561 gaaaccgccg acgccgacgc cgccaatgca gaggaagaag cgtaacctcg cggttgcgaa
     7621 cgtcgaggat tgttgaaaat cggtgaaacc catcacacgc gtaatgaact tcagatggcg
     7681 cgaacgggcg gccagacggc gcaagtaatc cgcgcagcac cgcccatcat ccccccgccc
     7741 cccgcaccct cccccattcc agcccgaacc gcgccagata c
//
LOCUS       DAOQIE010000188         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4880_length_7770_cov_7.477382, whole genome shotgun sequence.
ACCESSION   DAOQIE010000188 DAOQIE010000000
VERSION     DAOQIE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_7770_cov_7.477382"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1002
                     /locus_tag="PK144_11960"
     CDS             109..1002
                     /locus_tag="PK144_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66491.1"
                     /translation="MYLPAQFVNAYVDLFTWILDGSDWSFTGTPILHYLQSGKKPQSP
                     NAQLAHQRFMSRLAQVDPKFTTAKTFNYNDYDYINLSIRRCFIGKACPWEIQETLQLA
                     SELGLTNPDTVYKYCSDNLGVDCGGFVAAYWGEGVPHMADTQPVGSLGFLPRTFWSDS
                     HLWPDVRARRRKSAGEISPGDAAIFFKDIKGDDPDIAKQRDSSGTLIEGTGSEAFHIG
                     LVNSVSGGGDKITQLEIAESSGAASKFGSDGVNVRFASVQSSGIARGLIYAQTGANER
                     IYFLGPPAGWGPELPYTYGEE"
     gene            complement(1093..1755)
                     /locus_tag="PK144_11965"
     CDS             complement(1093..1755)
                     /locus_tag="PK144_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HNF66492.1"
                     /translation="MTARSLLCRRVLCLPVLLLVTALAGAREPYGFRAGVFDDYLLAL
                     SWSPTYCLTHADDAGQCGASKGYGFVLHGLWPQNRSGPYPQDCATRESLTPEATAFGA
                     TIFPSRSLIAHEWQKHGSCSGLDALGYFRLADQARTAIRVPDVFESPRRAQTTTAAGI
                     VAAFVSANPGLPAAAVVPVCRGMVFAELRICLDRQLSPLACAGNVRSNCGSRPVRVPA
                     AR"
     gene            complement(1828..2289)
                     /locus_tag="PK144_11970"
     CDS             complement(1828..2289)
                     /locus_tag="PK144_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNF66493.1"
                     /translation="MISLPLLDPPYWNWWLLAVVLMALEMVAPGFFFLWMGIAAALVG
                     VLLAGFPGLPVEWQWLIFALLSVGAIVGWQFWQRRHPSETDTPTLNRRGEQAVGRILV
                     LDQPIVAGSGRARLGDSTWPIRGPDLPAGTRVRVVAVDGTVLRVEALAGDG"
     gene            complement(2286..3260)
                     /locus_tag="PK144_11975"
     CDS             complement(2286..3260)
                     /locus_tag="PK144_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF66494.1"
                     /translation="MVISGFGVFVIGLVILMILLLVLGVCSVPQGMEYTVERFGAYKR
                     TLDPGLNFIIPVVDRIGRRMNMMEQVLDVPSQEIITKDNAMIRVDGVVFFQVLDAARA
                     AYEVSNLEFATLNLTMTNIRTVMGSMDLDELLSKRDEINARLLTVVDEATAPWGLKVT
                     RIEIKDIAPPMDLVDSMARQMKAERDKRAAILEAEGKRQSEILKADGLKQAAILEAEG
                     RRESAFRDAEARERMAQAEARATQMVSEAIAANGAQAVNYFVAQKYVDALAQIGRAPN
                     QKVILMPLDTTGVLGSIAGIAELAQEAMKERAAPPVAGRVPPQPGQPS"
     gene            3655..3921
                     /locus_tag="PK144_11980"
     CDS             3655..3921
                     /locus_tag="PK144_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66495.1"
                     /translation="MSTTTNVRTGYALAAAAAALFAGGLIAPTLASAADGEVKCLGAN
                     ACKGQSACAGEHNSCKGQNACKGQGWINTSSAQECSSKGGKPIL"
     gene            4036..4860
                     /locus_tag="PK144_11985"
     CDS             4036..4860
                     /locus_tag="PK144_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019216183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNF66496.1"
                     /translation="MSLTGFGLGLRPDHYADILATHPAVDWFEALTENYLVPGGKPLS
                     WLTRVREHYPLVLHGVSLSIGSTAPLDRDYLQAVKALAARVEPEWISDHLCWTGAHGL
                     NLHDLLPLPYTEEAVAHVAARVREVQDVLGRQILLENVSSYAEFAHAPLTEWQFLSAV
                     AEAADCLILLDINNIHVGARNHGYDPLEYLDGVPAARIRQHHLAGHLDLGELVIDTHD
                     HPVADPVWALYAEAVRRYGPVPAMIERDDRIPPLAELLAELDHARAIARHALAEAA"
     gene            4862..5656
                     /locus_tag="PK144_11990"
     CDS             4862..5656
                     /locus_tag="PK144_11990"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNF66497.1"
                     /translation="MSANLPGLAPLLDAFQRHVLDDDPAVAEWVRATPALPAARRLAV
                     YHEAYRLRLVEVCTHDYAGLQTLIGEDAFAALVLDYARARPSVYRSARDFSAALPDWL
                     QDEPERLPAVAQEMLRFERALADALDAADAPQPGTEALAAIDPAGWGELVPLAHPALT
                     RLTLGWDVPALWAEMRAGAEARAPRRSEAPIEWAVWRAPDLMTQYVSLPVAEAAVLDA
                     ALAGDDFATICETLCARLPQQEVAAAAVTALCAALGRGQLAGLRSG"
     gene            5717..6292
                     /locus_tag="PK144_11995"
     CDS             5717..6292
                     /locus_tag="PK144_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNF66498.1"
                     /translation="MKLRSFLLSGIVLAAVAGSGGCGREARDAEGGTPLLRAAERGDL
                     VRVRHLLLDRAEPEAVDRCHWTPLMKAALNGHAEVVDTLLAGGALTETRDRAGYTALM
                     LAAQNGHVPAITRLLAAGAWIEAREPFRDTTALMLAARGGHAAAVEALLAAGADPAAT
                     DRAGLRAAGHALEAGHPQLAQRLADAGSQRH"
     gene            complement(6308..6883)
                     /locus_tag="PK144_12000"
     CDS             complement(6308..6883)
                     /locus_tag="PK144_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HNF66499.1"
                     /translation="MPDSDPSRLLAQAGPRRGRPPLERWQPPLSGDIDIRIDRDGRWW
                     HEGRPIEREALVQLFAGILRREADGGHYLVTPIEKWRITVEDAPLLAGPPERLDGPAG
                     PRLRFTTSVGDVFDAGPQYPIEVEYRGDEPRPYLHVHHGLRARLTRAAFVELAGLAEP
                     ASLGGREVLGVYSDGAFFVLGPAGEGGEGGN"
     gene            6991..7269
                     /locus_tag="PK144_12005"
     CDS             6991..7269
                     /locus_tag="PK144_12005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF66500.1"
                     /translation="MAKARARHILVDSEAACLDIKRQIEEGADFAEMARLHSSCPSKR
                     QGGDLGEFGPGMMVREFDEAVFSGEVGKVLGPVRTQFGWHLLEVTQRS"
     gene            7275..7673
                     /locus_tag="PK144_12010"
     CDS             7275..7673
                     /locus_tag="PK144_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66501.1"
                     /translation="MRASRLLLSAALAAALAGCTGLSLPAGKSSPDPVGLAKLDGLWR
                     GAGKADGLTVRIARAGSSQPRMMYGFRDEAPTQPQCTVRAPATLSCKHESGLTTTYTQ
                     SGDSTLDFTAQGPKEGDEVLRAQLSRMQEP"
BASE COUNT         1151 a         2770 c         2707 g         1142 t
ORIGIN      
        1 ttcgcgtcta ccgtctttcc gggcagcggg cgatccgggc ggcttcgatc cgtgcccgtt
       61 caggaggaac gaccctgacc cttcagccgc cggcatgagg tcatcgccat gtatcttccg
      121 gctcagttcg tgaacgccta cgtcgacctg ttcacgtgga ttctcgatgg cagtgactgg
      181 agtttcaccg gcacgccgat cctgcattac ctgcaaagcg gcaagaagcc gcagtccccg
      241 aatgcgcagc tggcccacca gcgtttcatg agccggctcg cgcaggtcga tccgaagttc
      301 acgacggcca agactttcaa ttacaacgat tacgattaca tcaacctgtc gatccgccgc
      361 tgcttcatcg gcaaggcctg cccgtgggaa atccaggaaa ccctgcagct cgcctcggaa
      421 ctcggcctga ccaatccgga taccgtctac aaatactgct cggacaatct cggcgtcgat
      481 tgcggcggtt tcgtcgcggc ctactggggc gagggcgtgc cgcacatggc cgatacccag
      541 ccggtcggca gcctcggctt tctgccgcgc accttctggt ccgacagcca tctgtggccg
      601 gacgtacgcg cgcgccggcg caagtcggcc ggtgaaatca gccccggcga tgcggcgatc
      661 tttttcaagg acatcaaggg cgatgatccg gacatcgcca agcagcgcga cagcagcggc
      721 acgctgatcg aaggcaccgg ctcggaagcc ttccacatcg gcctcgtcaa cagcgtcagc
      781 ggcggcggcg acaaaatcac gcagctggag attgccgaat cgagcggagc cgcttcgaaa
      841 ttcggcagcg atggtgtcaa cgtgcgcttt gcgtcggtcc agtcctcggg catcgccagg
      901 gggctgattt acgcgcagac cggcgccaac gaacgcattt acttcctcgg cccgccggcg
      961 ggctgggggc cggagctgcc gtatacctac ggcgaggaat gaacggcgcg ggcctcacac
     1021 gaatccggac gccggcagtg gggccggact tcgccggtac agttcttcat gccgatcacc
     1081 ggcagtgcct tctcagcgtg ccgccggcac ccgtaccggg cgcgacccgc agttgctgcg
     1141 cacgttgcct gcgcacgcga gcggcgacag ctgccggtcg agacagatgc gcagttccgc
     1201 gaacaccatg ccccggcaga ccggcacgac ggccgctgcc ggcaggccgg gattggcgct
     1261 gacgaaggcg gcgacgatgc cggcggccgt cgtcgtctgc gcccggcgcg ggctttcgaa
     1321 cacgtcgggc acgcggatgg cggtgcgcgc ctggtcggcg aggcggaaat agcccagcgc
     1381 atccagcccg ctgcagctgc cgtgcttctg ccattcgtgc gcgatcaggc tgcggctcgg
     1441 aaagatcgtc gcgccgaagg cggttgcttc cggcgtcagg ctttcgcggg ttgcgcagtc
     1501 ctgcggatac ggcccggagc ggttctgcgg ccacaggccg tgcagcacga agccatagcc
     1561 cttgctcgca ccgcactggc cggcatcgtc cgcgtgcgtc aggcagtagg tcggcgacca
     1621 ggacagcgcc agcaggtagt catcgaacac gccggcgcgg aaaccgtacg gctcgcgcgc
     1681 tccggcgagt gctgtgacga gcagcagcac cggcaggcag agcacgcggc ggcacagcag
     1741 cgatcgggca gtcatggcag cggtctccgg ggccttgccg gcagggtcgg ggggccggga
     1801 aggaaagcgc gccggccggg gccggattca gccgtcgccg gccagtgcct cgacgcgcag
     1861 caccgtgccg tcgacggcga cgacacgcac gcgcgtgccg gccggcaggt cggggccgcg
     1921 gatcggccag gtcgagtcgc cgagtctggc gcggccgctg ccggcgacga tcggctgatc
     1981 gagcacgagg atgcggccga cggcctgttc gccgcggcgg ttcagcgtcg gggtgtcggt
     2041 ctcggagggg tggcggcgct gccagaactg ccagccaacg atcgcgccga ccgacagcag
     2101 cgcgaagatc agccactgcc attcgaccgg cagccccgga aagccggcga gcagcacgcc
     2161 gaccagagcg gcggcgatgc ccatccacag gaagaagaag ccgggcgcga ccatctccag
     2221 cgccatcagc acgacggcga gcagccacca gttccagtac ggcggatcga gcagcggcag
     2281 gctgatcatg agggctgtcc gggctgcggc ggcacgcggc cggccaccgg cggcgcggcg
     2341 cgctccttca tggcttcctg cgcgagttcg gcgatgccgg cgatcgagcc gagcacgccg
     2401 gtggtgtcga gcggcatcag gatcaccttc tggttcgggg cgcggccgat ctgcgcgagc
     2461 gcatcgacgt acttctgcgc gacgaagtag ttgacggcct gcgcgccgtt ggcggcgatg
     2521 gcctcggaga ccatctgcgt ggcgcgggcc tcggcctgcg ccatgcgctc gcgcgcctcg
     2581 gcatcgcgga aggccgattc gcgccggccc tcggcttcga ggatcgccgc ctgcttgagg
     2641 ccgtcggcct tgaggatctc ggactggcgc ttgccttcgg cttcgaggat cgcggcgcgc
     2701 ttgtcgcgct cggccttcat ctgccgggcc atcgaatcga cgaggtccat cggcggcgcg
     2761 atgtccttga tctcgatgcg cgtgaccttg aggccccagg gcgcggtggc ctcgtcgacg
     2821 acggtcagca gccgggcgtt gatctcgtcg cgcttggaga gcagctcgtc gaggtccatc
     2881 gagcccatga ccgtgcggat gttggtcatg gtcaggttca gcgtcgcgaa ttcgaggttg
     2941 ctgacctcgt acgcggcgcg cgcggcgtcg agcacctgga agaacaccac gccgtcgacg
     3001 cggatcatgg cgttgtcctt ggtgatgatc tcctgcgagg gcacgtcgag cacctgctcc
     3061 atcatgttca tgcgccggcc gatgcgatcg acgaccggga tgatgaagtt cagccccggg
     3121 tcgagcgtgc gcttgtaggc accgaagcgc tcgaccgtgt actccatgcc ctgcggcacc
     3181 gagcacacgc cgagcacgag cagcaggatc atcaggatca cgaggccgat gacgaaaacc
     3241 ccgaagccgc tgatcaccat gatgcgttct cccgagtggt ggacgatgcg cagaccctag
     3301 caaacgccgc cggccgcggc tgccgcgacc ggccacggtc ggacacggcc gaccacgacc
     3361 ggccgcggcc cggagcggcc accgcggcag gccggcacac gcaagctatt gttggcgcgg
     3421 gtgcgacgcc gctggcatgg cgtgtgaaac agccggcttg cgccgcgccc gccggccggg
     3481 cgcgcgggac ccggaagatt tttcttcaaa aataattcca cggcccgcgc atccaccggc
     3541 gcagcggctg cgaatacggg gtgtcgaccg gcagcacgcc ggcgccgacc cgaaagcagt
     3601 ccgcacacac caccacaagc ctgccattcc acgttgcatc cggagatcaa gaccatgagc
     3661 accacgacca acgtccgtac cggttatgcc ctcgccgccg ccgctgccgc gctgttcgcc
     3721 ggcggcctga tcgccccgac cctcgccagc gccgccgacg gcgaggtcaa gtgtctcggc
     3781 gccaatgcct gcaagggcca gtcggcctgc gccggcgagc acaactcctg caagggccag
     3841 aacgcctgca agggccaggg ctggatcaac acctcctcgg cgcaggagtg ctcgtccaag
     3901 ggcggcaagc cgatcctctg atgcaccggc ggcgggcgcc ccggtcccgg gcgcccgccg
     3961 cgcggttttt ccgcttcccc gcttttccgt tttcgctttc ccgctgttcc accctgctgc
     4021 cacgagctgc cgaccatgtc gctgaccggc ttcggtctgg gcctgcgccc cgaccattac
     4081 gcggacatcc tcgccacgca cccggccgtc gactggttcg aggcgctgac cgagaactac
     4141 ctcgtgcccg gcggcaagcc gctttcgtgg ctcacgcgcg tgcgcgagca ctacccgctg
     4201 gtgctgcatg gggtatcgct gtcgatcggt tcgaccgcac cgctcgatcg cgattatctg
     4261 caggccgtga aggcgctggc agcgcgcgtc gaacccgagt ggatttccga tcacctgtgc
     4321 tggaccggtg cccacggcct gaacctgcac gacctgctgc cgctgcccta taccgaggag
     4381 gcggtcgcgc acgtcgcggc gcgcgtgcgc gaggtgcagg acgttctcgg ccggcagatc
     4441 ctgctcgaaa acgtgtcgag ctacgccgag ttcgcgcacg cgccgctgac cgagtggcag
     4501 ttcctgagcg cggtcgccga ggcggccgac tgcctgatcc tgctcgacat caacaacatc
     4561 cacgtcggcg cccgcaacca cggctacgac ccgctcgaat acctcgatgg cgtgccggcg
     4621 gcgcgcatcc gccagcacca tctggccggc cacctcgatc tcggcgaact cgtcatcgac
     4681 acccacgacc acccggtcgc cgatccggtc tgggcgctgt acgccgaggc cgtgcgccgc
     4741 tacgggccgg tgccggcgat gatcgagcgc gacgaccgca tcccgccgct cgccgagctg
     4801 ctcgccgaac tcgatcacgc gcgcgcgatc gcgcgccacg ccctcgccga ggccgcctga
     4861 gatgagtgcg aacctgcccg gcctcgcccc gctgctcgat gccttccagc gccacgtgct
     4921 cgatgacgat ccggcggtcg ccgagtgggt gcgcgccacg ccggcgctgc cggccgcgcg
     4981 ccggctggcg gtgtaccacg aggcctaccg gctgcggctg gtcgaggtct gcacgcacga
     5041 ttacgccggc ctgcagacgc tgatcggcga ggacgccttc gcggcgctgg tgctcgatta
     5101 cgcgcgcgcg cggccgtcgg tgtaccgctc ggcgcgcgat ttctcggcgg cgctgccgga
     5161 ctggctgcag gacgaacccg agcggctgcc ggcggtggcg caggagatgc tgcgcttcga
     5221 gcgcgcgctc gccgatgcgc tcgatgccgc cgacgcgccg cagcccggca ccgaggcgct
     5281 ggcggcgatc gatccggccg gctggggcga gctggtgccg ctcgcgcatc cggcgctgac
     5341 ccggctgacg ctcggctggg acgtgccggc gctgtgggcg gaaatgcgcg ccggcgccga
     5401 ggcgcgcgcg ccgcgccgga gcgaggcgcc aatcgaatgg gcggtgtggc gcgcgcccga
     5461 cctgatgacg cagtacgtgt cgctgccggt cgccgaggcc gcggtgctcg atgccgccct
     5521 cgccggcgat gacttcgcga cgatctgcga gacgctctgc gcgcggctgc cgcagcagga
     5581 agtcgccgcc gcggcggtca cggcgctgtg cgccgcgctc ggccgcggcc agctcgccgg
     5641 gctgcgctcc ggctgaaccg caggccgctg cgaatccgca gcgacgggcc gcggggccgc
     5701 cggcacactg gccggcatga agctccgatc cttcctcctg tccggcatcg tgctcgccgc
     5761 cgtggccggg tccggcggct gcgggcgtga agcgcgcgat gccgagggcg gcacgccgct
     5821 gctgcgcgcc gccgagcgcg gcgatctcgt ccgggtccgg catctgctgc tcgatcgcgc
     5881 cgaaccggaa gccgtggacc gctgccactg gacgccgctg atgaaggcgg ccctgaacgg
     5941 ccatgccgag gtcgtcgata ccctgctcgc cggcggtgcc ctgaccgaaa cgcgcgaccg
     6001 tgccggctat acggcgctga tgctggcggc ccagaacggc catgtgccgg cgatcacgcg
     6061 gctgctggca gccggagcct ggatcgaggc gcgcgagccg ttccgggaca ccacggccct
     6121 gatgctggcg gcgcggggcg gtcatgcagc ggccgtcgag gcgctgctcg ccgccggggc
     6181 cgatccggcg gcaacggacc gcgccggcct gcgcgcggcc ggacatgcgc tcgaagccgg
     6241 tcacccgcag ctggcgcagc ggctggcgga cgccggctcg cagcggcact gaaggcagcc
     6301 gaaagcgtca attcccgccc tccccgccct ccccggccgg accgagcacg aagaacgcgc
     6361 cgtcgctgta tacgccgagc acctcgcggc cgccgaggct cgccggttcg gcgaggccgg
     6421 ccagttcgac gaaggcggcg cgcgtcagcc gggcgcgcag gccgtgatgc acgtgcaggt
     6481 aggggcgcgg ttcgtcgccg cgatattcga cctcgatcgg gtactgcggc ccggcatcga
     6541 agacatcgcc gacgctggtc gtgaagcgca gccgcgggcc ggccggaccg tcgagccgct
     6601 cgggcgggcc ggcgagcagc ggcgcatcct cgaccgtgat ccgccacttt tcgatcggcg
     6661 tgaccaggta atgcccgccg tcggcctcgc gacgcaggat gccggcgaac agctgcacca
     6721 gcgcctcgcg ttcgatcggc cggccttcgt gccaccagcg gccgtcgcgg tcgatgcgga
     6781 tgtcgatgtc gccgctgagc ggcggctgcc agcgttcgag cggcgggcgg ccgcggcggg
     6841 ggccggcctg cgcgagcagg cgggacggat cgctgtccgg catgtcatat cctccgcggc
     6901 cgttgcatcg ccggtcgctt gctgttcaag ccgccggacc gccgctaagc tcccggcccc
     6961 gtttcccatg agcagatgga gagattcccc atggccaaag cccgtgcccg acacatcctc
     7021 gtcgattccg aggcggcctg cctcgacatc aagcgccaga tcgaggaagg cgccgacttc
     7081 gccgagatgg cccgcctgca ttcctcgtgt ccgtcgaagc gccagggcgg cgacctcggc
     7141 gagttcggcc ccggcatgat ggtgcgcgaa ttcgacgaag ccgtgttcag cggcgaggtc
     7201 ggcaaggtgc tcggcccggt ccggacgcag ttcggctggc acctgctcga agtcacgcag
     7261 cggagctgat cgtgatgcgt gcgtcccgtc tgctgctgtc cgccgcgctc gcggccgccc
     7321 tcgccggctg caccggcctg tcgctgccgg ccggcaagtc gtcacccgac ccggtcgggc
     7381 ttgcgaagct cgacggtctc tggcgcggtg ccggcaaggc cgatggcctc accgtgcgca
     7441 tcgcacgcgc cggttcctcg cagccgcgca tgatgtacgg cttccgcgac gaggccccga
     7501 cgcagccgca atgcacggtg cgcgcgccgg cgacgctgag ctgcaagcac gagagcgggc
     7561 tgacgacgac gtatacccag tccggcgaca gcacgctcga tttcacggcg cagggcccga
     7621 aggaaggcga cgaggtgctg cgcgcgcagc tctcgcgcat gcaggagccc tgatcccgcg
     7681 cccccgcagc cagccgtcac gataaaaaag cccgggcatc caccccgggc tttttttgtg
     7741 gctgcggctt cgtgcgggag cctcagcgct
//
LOCUS       DAOQIE010000189         7717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4916_length_7717_cov_7.025972, whole genome shotgun sequence.
ACCESSION   DAOQIE010000189 DAOQIE010000000
VERSION     DAOQIE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_7717_cov_7.025972"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /gene="ilvD"
                     /locus_tag="PK144_12015"
     CDS             <1..1808
                     /gene="ilvD"
                     /locus_tag="PK144_12015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNF66502.1"
                     /translation="GARALWRATGMKDGDFGKPIIAVANSFTQFVPGHVHLKDLGQLV
                     AREIEAAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADAVEYMVNAHCADALVC
                     ISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKTRLAGQDVKLDLVDAMVMAADSKA
                     SDEEVAQVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGTLLATHADRRELFLE
                     AGRRIVTLARRHYEEGDHSVLPRAIANFQAFENAMSLDIAMGGSTNTVLHLLAAAQEG
                     GVQFTMSDIDRLSRRVPNLCKVAPATQKYHIEDVHRAGGIMAILGELDRAGLLHHDVK
                     TVHSDTLAAALAANDIAVATCTDAARKRYLAGPAGIPTQEAFSQDCRWPTADADRKNG
                     CIRDRAHAYSQDGGLAVLFGNLAPDGCIVKTAGVDESILKFTGPARIMESQEDAVAAI
                     LNDEIRAGDVVLIRYEGPKGGPGMQEMLYPTSYLKSRGLGKACALITDGRFSGGTSGL
                     SIGHVSPEAAEGGNIALVEEGDPIEIDIPKRSIRIVVSDKELSERRSRMTASGARAWK
                     PVSRERVVSTALKAYAAMTTSASRGAVRDLSQLER"
     gene            1894..2292
                     /locus_tag="PK144_12020"
     CDS             1894..2292
                     /locus_tag="PK144_12020"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNF66503.1"
                     /translation="MDQQRLQERIAAFLKALGQLERAARQPKDEFLRDSVIQRFEFTH
                     ELAWKMLKLRLEAELVLARTPKETLQEALKAGFLDDGSGWSVLQRMRNLTSHTYDESL
                     AETVYAFAVGEGLELYRRLAVQARAWQMTD"
     gene            2274..2657
                     /locus_tag="PK144_12025"
     CDS             2274..2657
                     /locus_tag="PK144_12025"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF66504.1"
                     /translation="MADDGLSFGLSAEILEWLRTVFARYPGIERVLVFGSRAKGTYRN
                     ASDIDLAVLAPGMDEKTFAQLWNDLDDLPLVFTLDVLHWDRLPAGRIRDHVIRDGRQV
                     YPVAEPVRMPGAPSPLAEPVPGPDG"
     gene            complement(2672..3631)
                     /gene="corA"
                     /locus_tag="PK144_12030"
     CDS             complement(2672..3631)
                     /gene="corA"
                     /locus_tag="PK144_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNF66505.1"
                     /translation="MLIAFLIRDGRLEQTPLHVQDDAPIPPDLMQQIVWVDLNEPTEE
                     EREWVEAQFGLELPEYREMNDLEPSARLYEDEDGLHLHSYFLSDQEEQPRNITVAFAV
                     LGGRLFTLHEDDLSTFRLFRLRARRQIGLVKDVGDIILGLFEAKVEHIADKLEEIYSE
                     LEALSHRVLAETDSDMEDVLSGLAVQEDMNGKARLILLDTRRTLSFLLRYAIAGRGED
                     IREILRDVESLTSHTAFLFDKVNFLMDAAMGFINIEQNKIIKIFSIAAVVFLPPTLVA
                     SLYGMNFQHMPELEWEYGYPMAICLMILAGVAPYWFFKRKGWL"
     gene            3757..4101
                     /locus_tag="PK144_12035"
     CDS             3757..4101
                     /locus_tag="PK144_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF66506.1"
                     /translation="MEQPDSGNLFAGIPARLDAEAFGLLYRSPGVRIERILSRGQSTP
                     PGAWYDQPGDEWVVLLAGHAELEFADGRRRALLPGDWLLLPAHCRHRVAATSTEPAAV
                     WLAVHIEPGPPA"
     gene            complement(4116..4574)
                     /locus_tag="PK144_12040"
     CDS             complement(4116..4574)
                     /locus_tag="PK144_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF66507.1"
                     /translation="MSVKPIPDGYTAVTPYLVVRDAASLLGFIQAAFDAELVMRMPRP
                     DGALAHAELRIGGAALMLGEACEQLGEMPASLYLYLPDVDAAYARALTAGATAVRAPE
                     DMFYGDRVASVRDAHGNLWSIGTHIEDVPPEELPARAAAAWGQPPSGVAH"
     gene            complement(4663..>7717)
                     /locus_tag="PK144_12045"
     CDS             complement(4663..>7717)
                     /locus_tag="PK144_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66508.1"
                     /translation="TAVSLYLHARLQSHMRSTAEQMQVIAAAHARAIEEWWLLHESNA
                     GLLALAGGAHSPVHRLKLDAQRKSRANTWLQSIVLTHDYLSAVLLDASGMPVAESVRP
                     GSPLVSGVAPGAFAQLLAREEGGYLPATDGLSLLWLKPLGVEAGWLVLRHGLQEVQAL
                     LDPGPQALPGLQLRLVQHTAQGMQYLQLPSGAEPAKPDDPLIARLPAPGHPAELLTDT
                     LGPDGQPVLAAIHGNIGPDWTLVALMSRQAILEALHPQIIQTLVMAVITSLVCTLAMW
                     SARRHQIDAALRVSHDELQRVVEARTAELCQSLERLHAEAVRREQVQAERDGLFSLAG
                     DLLAIVDDKGMFLRVNPAWERTLGHPVVQIEGTHWLDWLHPDDVQRSLETAAQLAGGQ
                     PLQHFANRCRHADGGWRWLEWSAVRDPQHGHIYAVARDVTERRENEQKLRLAHRGLDA
                     SPNSVLIADAVQPGLIVLYANPAYERLSGRPLAGVIGQPCDFMTDADTDPQALVELRA
                     ALAKGCDTRIEMQCRRADGKPFWTNLHLAPVREPHGRISHWVVIQQDVTARRSIEARL
                     VHQAGHDALTGLPNRTRLRERLKHALARARRSGGRIAVLFVDLDRFKTINDSLGHAVG
                     DALLCEVAQRLKQSVRTGDLVARLGGDEFVVLAEHIGRPDDAARVARKLIEVIELPYA
                     VAGREFVLSASIGIGLYPDDAGDLDTLLRVADTAMYGVKHGGRNGYGYYEPAMHERAL
                     AALERERDLRGALERGEFELHYQPQVELASGRIVAVEALLRWRHPQRGLVPPDQFIPI
                     AEESGLIVPIGDWVTRTACAQLGEWQRLGLGPLRMAVNLSARQFRRAEIVAETLAAVE
                     AAGIAPQSLELEITESLLMDDVERALTRMHALGDRGLRLAMDDFGTGYSSLAFLKRFP
                     VGMLKIDRLFVRDLTENAANAAIARTIVQLGQNLGLTVLAEGVEEAEQAEMLRGFGCT
                     LIQGWLTGRPQPAADLRKLLEAQRAGQTVLPPAPPPARVVALRAG"
BASE COUNT         1208 a         2638 c         2712 g         1159 t
ORIGIN      
        1 cgggcgcccg cgcgctgtgg cgcgctaccg gcatgaagga cggcgacttc ggcaagccga
       61 tcatcgcggt cgccaactcc ttcacgcagt tcgtgcccgg ccacgtgcac ctgaaggacc
      121 tcggccagct cgtcgcgcgc gagatcgagg ccgccggcgg catcgccaag gaattcaaca
      181 ccatcgccgt cgacgacggc atcgcgatgg ggcacggcgg catgctgtac tcgctgccct
      241 cgcgcgagct gatcgccgat gccgtcgagt acatggtcaa cgcccactgc gccgacgcgc
      301 tggtgtgcat ctcgaactgc gacaagatca cgcccggcat gctgatggcg gcgatgcgcc
      361 tgaacatccc ggtggtgttc gtctccggcg ggccgatgga agccggcaag acgcgcctcg
      421 ccggtcagga cgtgaagctc gatctggtcg acgcgatggt catggccgcc gattcgaagg
      481 caagcgatga agaggtcgcc caggtcgagc gttcggcctg cccgacctgc ggctcgtgct
      541 cgggcatgtt cacggccaac tcgatgaact gcctgaccga ggcgctgggc ctgagcctgc
      601 ccggcaacgg cacgctgctc gcaacccatg ccgaccggcg cgagctgttc ctcgaagccg
      661 gccggcgcat cgtgacgctg gcgaggcgcc actacgaaga gggcgatcac tcggtgctgc
      721 cgcgcgcgat cgccaacttc caggccttcg agaacgcgat gagcctcgac atcgccatgg
      781 gcggctcgac caacaccgtg ctgcacctgc tggccgcagc ccaggaaggc ggcgtgcagt
      841 tcacgatgag cgacatcgac cgcctgagcc ggcgcgtgcc gaacctctgc aaggtcgcgc
      901 cggcgacgca gaagtaccac atcgaggacg tgcaccgggc cggcggcatc atggccatcc
      961 tcggcgagct cgatcgcgcc ggcctgctgc accacgacgt gaagaccgtg cacagcgaca
     1021 cgctcgccgc cgcactcgcc gccaacgaca tcgccgtggc gacctgcacc gacgccgcgc
     1081 gcaagcgtta tctcgccggc ccggccggca ttccgacgca ggaagccttc agccaggact
     1141 gccgctggcc cacggccgat gccgaccgca aaaacggctg catccgcgac cgggcgcacg
     1201 cctattcgca ggacggcggt ctggcggtgc tgttcggcaa cctcgcgccg gacggctgca
     1261 tcgtcaagac ggccggcgtc gacgagtcga tcctgaagtt caccggcccg gcgcggatca
     1321 tggaaagcca ggaggacgcc gtcgccgcga tcctgaacga cgagatccgc gccggcgatg
     1381 tcgtgctgat ccgctacgaa ggtccgaagg gcggcccggg catgcaggag atgctgtatc
     1441 cgaccagcta cctgaagtcc aggggcctcg gcaaggcctg cgcgctgatc accgacgggc
     1501 gcttttcggg cggcacctcg ggcctgtcga tcggccacgt cagccccgag gcggccgagg
     1561 gcggcaacat cgcgctggtc gaggagggcg atccgatcga gatcgacatc ccgaagcgca
     1621 gcatccgcat cgtcgtgtcc gacaaggagc tctccgaacg ccgcagccgc atgaccgcga
     1681 gcggtgcccg ggcctggaag ccggtcagcc gcgagcgcgt cgtgtcgacg gccctgaagg
     1741 cctacgcggc gatgacgacc tcggcgagcc gcggcgcggt gcgggacctg tcgcagctgg
     1801 agcgctgagc gaggctgacc ggacgcagca ccggcggcgt gcagggacgc tgccggtgct
     1861 gcggcggcgc agaaccggga gcacgcaagc cggatggatc agcaacgcct gcaggaacgc
     1921 atcgccgcct ttctgaaggc gctcggacag ctggagcgcg cggcccggca gccgaaggac
     1981 gaattcctgc gcgattcggt catccagcgc ttcgagttca cgcatgaact cgcctggaaa
     2041 atgctgaagc tgcggctcga agccgagctc gtcctggcgc gcacgccgaa ggaaacgctg
     2101 caggaggcgc tgaaagccgg gttcctcgac gatggcagcg gctggagcgt cctgcagcgc
     2161 atgcgcaacc tgaccagcca tacctacgat gaatcgctcg ccgaaacggt gtacgccttc
     2221 gccgtcggcg aggggctgga actgtatcga cggctcgccg tacaggcccg tgcatggcag
     2281 atgacggact gagcttcggc ctgtcagccg agattctcga atggctgcgt accgtgttcg
     2341 cgcgttatcc cggcatcgag cgcgtactgg ttttcggctc gcgtgcgaaa ggcacctacc
     2401 gcaacgcctc ggacatcgac cttgccgtcc tcgccccggg gatggacgaa aagaccttcg
     2461 cgcagctttg gaacgatctc gacgatctgc ccctggtgtt cacgctcgat gtcctgcact
     2521 gggaccggtt gccggccgga cgcatccgcg atcatgtgat ccgcgatggc aggcaggtgt
     2581 acccggtcgc agaaccggta cggatgcccg gcgcaccaag tcctctggcc gagcctgtgc
     2641 ccgggccgga tggctgaccc tcagctgctt gctacagcca gcccttcctc ttgaagaacc
     2701 agtacggcgc cacgccggcc aggatcatca ggcagatcgc catcgggtag ccgtattccc
     2761 attccagctc cggcatgtgc tggaagttca tgccgtacag cgaggcgacc agcgtcgggg
     2821 gcaggaacac gacggcggcg atcgagaaga tcttgatgat cttgttctgc tcgatgttga
     2881 tgaagcccat cgccgcgtcc atcaggaagt tgaccttgtc gaacaggaag gcggtgtggc
     2941 tggtcagcga ttcgacatcg cgcaggatct cgcggatgtc ctcgccgcgg ccggcgatcg
     3001 cgtagcgcag caggaaggac agcgtgcggc gcgtgtcgag caggatcagg cgtgccttgc
     3061 cgttcatgtc ctcctgcacg gccagccccg agagcacgtc ctccatgtcc gaatcggtct
     3121 cggcgagcac gcggtggctc agggcttcga gttcggagta gatctcttcg agcttgtcgg
     3181 cgatgtgttc gaccttggcc tcgaacaggc cgaggatgat gtcgccgaca tccttgacca
     3241 gcccgatctg gcggcgcgcg cgcagccgga acagccggaa ggtcgaaagg tcgtcctcgt
     3301 gcagcgtgaa cagccggccg ccgagcacgg cgaaggcgac cgtgatgttg cgcggctgtt
     3361 cctcctgatc gctcaggaag tacgaatgca ggtgcaggcc gtcctcgtcc tcgtacaggc
     3421 gcgccgaggg ttcgagatca ttcatctcgc ggtactcggg cagttccagc ccgaactgcg
     3481 cctcgaccca ctcgcgctcc tcctcggtcg gctcgttgag atcgacccag acgatctgct
     3541 gcatgaggtc cggcgggatc ggcgcgtcgt cctgcacgtg cagcggcgtc tgctccagac
     3601 gaccgtcgcg gatcaggaag gcgatcagca tggggcgggc tcccggagcg tggcggctga
     3661 cgtgggcagg aaggcggccg cgagtgtatc cggcgcccgg aaacggtgca tccttgccac
     3721 atgcagggca ggggaatgga tcagggggcg aggaacatgg aacagccgga cagcggcaat
     3781 ctgtttgcgg gcattcctgc ccgtctcgat gcggaagcct tcgggctgct gtaccggagc
     3841 cccggcgtgc gcatcgaacg catcctgtcg cgcggccaga gtaccccgcc cggggcctgg
     3901 tacgaccagc ccggcgacga atgggtggtg ctgctcgccg gtcacgccga actggagttt
     3961 gccgatggca ggcgccgggc gctgctgccc ggcgactggc tgctgctgcc ggcgcactgc
     4021 cggcaccggg tggcggcgac cagcaccgag ccggcggcgg tatggctggc ggtgcacatc
     4081 gaacccggcc cgccggcgtg agcggccggc gtgactcagt gcgccacgcc cgagggcggc
     4141 tgaccccagg ccgcggcggc gcgggccggc agttcttcgg gcggcacgtc ctcgatgtgc
     4201 gtgccgatcg accacaggtt gccgtgggcg tcgcgcacgc tggcgacgcg atcgccgtag
     4261 aacatgtctt cgggtgcgcg taccgccgtg gctccggcgg tcagcgcacg cgcgtaggcg
     4321 gcatcgacat ccggcaggta caggtacagc gaggccggca tctcgcccag ctgttcgcag
     4381 gcttcgccga gcatcagcgc cgcaccgccg atgcgcagct ccgcatgggc gagcgcacca
     4441 tccgggcgcg gcatgcgcat caccagctcg gcgtcgaacg cggcctggat gaacccgagc
     4501 agcgacgcgg catcgcgcac gaccagatac ggggtgacgg cggtgtaacc gtccggaatc
     4561 ggcttgacgc tcatggcagg gttcctcggt ggccgggcat ggcccctgtt tctgagccgc
     4621 cggcggccgt gaggttcgcc tgcaggccac gaaggccgcc gttcagcccg cgcgcaaggc
     4681 cacgacccgc gccggcggcg gggccggcgg cagcacggtc tgaccggccc gctgggcttc
     4741 gagcagtttg cggaggtctg cggccggctg cggccggccg gtcagccagc cctggatcag
     4801 cgtgcagccg aagccgcgca gcatctcggc ctgctcggcc tcctcgacgc cctcggcgag
     4861 cacggtcagg ccgagattct ggccgagctg cacgatggtg cgcgcgatgg cggcgttggc
     4921 ggcgttttcg gtgagatcgc gcacgaacag ccggtcgatc ttcagcatcc cgaccgggaa
     4981 gcgcttcagg aaggcgagcg acgaatagcc ggtgccgaag tcgtccatcg ccagtcgcag
     5041 gccgcggtcg ccgagtgcgt gcatgcgggt cagcgcgcgt tcgacatcgt ccatcagcag
     5101 gctctcggtg atctccagtt ccagcgattg cggcgcgatg ccggcggctt cgaccgcggc
     5161 cagcgtctcg gcgacgatct cggcgcggcg gaactgccgg gccgacaggt tcacggccat
     5221 gcgcagcggt ccgagcccga ggcgctgcca ttcgccgagc tgcgcgcagg cagtgcgcgt
     5281 gacccagtcg ccgatcggca cgatcagccc gctttcctcg gcgatcggga tgaactggtc
     5341 cggcggcacc aggccgcgct gcggatgacg ccagcgcagc agcgcctcga ccgcgacgat
     5401 gcgcccgctc gcgagttcca cctgcggctg gtagtgcagt tcgaactcgc cgcgctccag
     5461 cgccccgcgc aggtcgcgtt cgcgttcgag cgcggcgagc gcccgctcgt gcatcgccgg
     5521 ttcgtagtag ccgtagccgt tgcgaccgcc gtgcttgacg ccgtacatcg cggtgtcggc
     5581 aacgcgcagc agcgtgtcga ggtcgccggc atcgtccgga tacaggccga tgccgatgct
     5641 cgccgacagc acgaactcgc gcccggcgac ggcgtagggc agctcgatga cctcgatcag
     5701 cttgcgcgcc acgcgcgcgg cgtcgtcggg ccggccgatg tgctcggcga gcacgacgaa
     5761 ctcgtcgccg ccgaggcgcg cgaccagatc gccggtgcgc acgctctgct tcaggcgctg
     5821 ggcgacctcg cagagcagcg catcaccgac cgcgtggccg aggctgtcgt tgatggtctt
     5881 gaagcggtcg agatcgacga acagcaccgc gatgcgcccg ccgctgcgcc gcgcgcgtgc
     5941 cagcgcgtgc ttgaggcgtt cgcgcaggcg cgtgcggttc ggcagcccgg tcagcgcgtc
     6001 gtgcccggcc tgatgcacga gtctggcctc gatgctgcgc cgcgcggtga cgtcctgctg
     6061 gatcacgacc cagtgcgaga tccgtccgtg cggctcgcgc accggtgcca ggtgcaggtt
     6121 ggtccagaac ggcttgccgt cggcgcgccg gcactgcatc tcgatgcgcg tgtcgcagcc
     6181 tttggcgagc gcggcgcgca gctcgacaag ggcctgcgga tcagtgtcgg catcggtcat
     6241 gaaatcgcac ggctggccga tgaccccggc gagcggccgg ccgctcaggc gctcgtaggc
     6301 cggattggcg tacagcacga tcagccccgg ctgcacggca tcggcgatca gcacgctgtt
     6361 cggtgaggca tcgaggccgc gatgcgccag acgcagcttc tgttcgtttt cgcgccgttc
     6421 ggtgacatcg cgcgccaccg cgtagatgtg gccgtgctgc ggatcacgca ccgccgacca
     6481 ctccagccag cgccagccgc cgtcggcatg ccggcagcgg ttggcgaaat gctgcagcgg
     6541 ctggccgccg gccagctgcg cggcggtctc caggctgcgc tgcacgtcat ccggatgcag
     6601 ccagtcgagc cagtgcgtgc cttcgatctg caccaccgga tggccgagcg tgcgctccca
     6661 ggccggattc acgcgcagga acatgccctt gtcatcaacg atcgccagca ggtcaccggc
     6721 gagcgagaac aggccgtcgc gttcggcctg cacctgttcg cggcgaacgg cctcggcgtg
     6781 caggcgctcc aggctctggc acagctcggc ggtgcgggcc tcgaccacgc gctgcagttc
     6841 gtcgtgcgag acgcgcagcg ccgcatcgat ctggtggcgt cgcgcgctcc acatcgccag
     6901 cgtgcagacc agcgaggtga tgacggccat gaccagggtc tggatgatct gcgggtgcag
     6961 ggcttccagg atggcctggc gcgacatcag cgcgaccagc gtccagtccg gaccgatgtt
     7021 gccatggatg gccgcgagca ccggctggcc gtccgggccg agggtatcgg tcagcagttc
     7081 ggccggatgg ccgggggccg gcaggcgggc gatcagcgga tcgtcgggct tcgccggttc
     7141 tgcgccggac ggcagttgca ggtactgcat gccctgcgcc gtatgctgga ccagccgcag
     7201 ctgcaggccg gggagggcct gcggaccggg atcgagcagc gcctgcactt cctgcaggcc
     7261 gtgacgcagc accagccagc cggcctcgac cccgagcggt ttcagccaca gcaggctcag
     7321 gccgtcggtc gccggcagat aaccgccctc ttcgcgggcg agcagctggg caaaggcgcc
     7381 gggggcgacg ccggacacga gcggcgaacc cgggcgcacc gactcggcca ccggcatgcc
     7441 gctcgcatcg agcagcacgg cactcaggta gtcgtgggtc agcacgatcg actgcagcca
     7501 ggtattggcg cgggacttgc gctgcgcgtc gagcttcagc cggtgcaccg gcgagtgcgc
     7561 gccaccggcg agcgccagca ggccggcgtt gctctcgtgc agcagccacc attcctcgat
     7621 cgcccgggcg tgcgcggccg cgatcacctg catctgctcc gccgtgctgc gcatgtgcga
     7681 ctgcaggcgg gcgtgcaggt acaggctgac cgccgtc
//
LOCUS       DAOQIE010000190         7712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4925_length_7712_cov_7.357973, whole genome shotgun sequence.
ACCESSION   DAOQIE010000190 DAOQIE010000000
VERSION     DAOQIE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4925_length_7712_cov_7.357973"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PK144_12050"
     CDS             <1..1145
                     /locus_tag="PK144_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNF66509.1"
                     /translation="FTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFATSMVGHWHG
                     GLGLAGVIACALFAAVSGSSPATVVAIGSILLPAMLKQGFPNRFGAGVIATSGALGIL
                     IPPSIVMVMYSVATNTSVGALFMAGVVPGLMLAGFLAFTTWYRAKKFGYPRQPKATMQ
                     ERIKAFRESVWGLLLIIIVIGGIYAGLFTPTEAAAMSAVYAFIVAVFIYKDMGLKDVP
                     KVLLSSANMSAMLLYIITNAVMFSFIMTDQKIPQTLAEIMIGSGLGPITFLLMCNIVL
                     LLAGNVMEPSSIVLIFAPILFPIAMQLGIDPVHFGILIVVNMEVGMCHPPVGLNLYVA
                     SGITKMGITELTIAVWPWLLTMLVFLVIVTYVPAISLTLPKLLGVM"
     gene            complement(1249..1683)
                     /locus_tag="PK144_12055"
     CDS             complement(1249..1683)
                     /locus_tag="PK144_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF66510.1"
                     /translation="MSQSASALQHMVRLAQRLSTAFEAAVGMHLARWRILNHLHEHGA
                     STQKHMKTLIGVDAAALTRQLQLLEAEGLVRRRSHPDDQRLTEVALTAAGTDRLAAGR
                     VARDAFVARLLAGLDAEALAAFEATLAHIGRNLDARTQEPDA"
     gene            complement(1680..3302)
                     /locus_tag="PK144_12060"
     CDS             complement(1680..3302)
                     /locus_tag="PK144_12060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66511.1"
                     /translation="MSAVPLAGTEPVPAAGTAAPAGLYGAQALRAMLGIALVAMLVAL
                     DQTVVGTAMPTIVAELRGFEQFAWVATAYLLSSTTLLPVLGRLGDLFGRKPFVIAAII
                     VFTGASALCGQAGSMPALIAARALQGVGGAMIIATAFTSIADLFPDTAHRLRWQALMS
                     SSFALASGIGPVLGGLLTEHFGWRSVFYVNLPLGLLALVLVIAYLPRSRGTRAEGAGF
                     DLPGALWLSGGLAALLLWVELGQRAGAWFAGALPLWLAGGVLALLLFVRRQLHARHPL
                     LPLRLLAIPRVRRVVLCALAAGAVMFAALFYAPLLLQGAYGHSPHEAGLIVTPLVLAI
                     ALGSLINGRLYGRLADPESLLLPGWLLLGAGVLGVQAVGAQTSAWLAAGAFLMCGLGL
                     GFLFPNFTLLVQTSVPRAELGVATALVQTVRALGSMAGTVVLGVLVTGTWRTAMDAAG
                     SGAGAQALRPLFADPQLLLDPAGLHQATTLAARLPDGPAQLAQLLDAAHRALAAGIHL
                     GLLLAAGLAALVLIALLAGRWRRRGRVAEAGT"
     gene            complement(3486..4511)
                     /locus_tag="PK144_12065"
     CDS             complement(3486..4511)
                     /locus_tag="PK144_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNF66512.1"
                     /translation="MTTSKSVSLPLIEIDQPLLDAAEPCPQRRGFLRTAGLLVSSLIA
                     LPVLARSAGNGKHTVSTARSTAKSASASTARTSARAAKPAAGASVAGRAATGKRMPAK
                     PVIALRQAPAGKSNLKIRVDARGKPVGGRVVAQIRGGSTYKPQLRSTGRGYVDRGEQR
                     IGRGSIVADTRGLEAPGIITESKTASTESLFRNGDRVLSLYAPGTGESVREVFWTPNT
                     GYLPQSIKEISWALRDHHNDEFRLFDPRALDILYAMQLMLQRPETHIISGYRSPETNA
                     LLREESRGVAKNSFHMQARALDVRMPGLGVGQMRMAALSLRAGGVGYYPRSNFVHIDS
                     GPVRSWG"
     gene            4675..5163
                     /locus_tag="PK144_12070"
     CDS             4675..5163
                     /locus_tag="PK144_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNF66513.1"
                     /translation="MSKAKTPVTAAIRLLRQHKVGFSDHLYEYEERGGTAHSAHALGL
                     PEHAVVKTLIMEDDRHEPLIVLMHGDLQVSTKELARQIGARSIAPCAPEVANRHSGYL
                     VGGTSPFGTRHAMPVYVEATILELPRIWINGGKRGYLIGIEPSVLSTVLKPLPVRVGI
                     PG"
     assembly_gap    5181..5280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5281..5450)
                     /locus_tag="PK144_12075"
     CDS             complement(<5281..5450)
                     /locus_tag="PK144_12075"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNF66514.1"
                     /translation="MDEQRLIDLEIRYAHQDAALNELSDLVYRQGRELDALRRLVGDL
                     RRQLQAMAPSNIA"
     gene            5549..>7712
                     /gene="rlmKL"
                     /locus_tag="PK144_12080"
     CDS             5549..>7712
                     /gene="rlmKL"
                     /locus_tag="PK144_12080"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HNF66515.1"
                     /translation="MSADLPFFATAPRGVEPLLAAELAALGAAPVREGRGGVSFGGPL
                     ETAYRVCLWSRTASRVLRLLARVPAADADALYAGVLDLPWEDHLDPDGTLAVDFVAAG
                     RSAIRHTQFGAQRVKDAVCDRLRERCGRRPSVDRAAPDLRINVHASGETADVALDLSG
                     ESLHRRGYREDGALAPLKENLAAALLLKAGWPAIVEAGGALLDPMCGSGTFVIEAALI
                     AADRAPGLLRPDWGFFGWRGHDAGLWRQLVLEARERAAAGLAKPLPSLVGSDHEARAI
                     RAALTNAGQAEIAKLVHFERRELAGAVPPKGAPGLVIVNPPYGERLGELEALADTYAA
                     LGDVLKARFGGWQAAVFTGNPDLGKRMGLRAGKVHAFYNGPIECRLLRFEVTPEQHVD
                     REAVDRRRAGQAIERALAAGADALVNRVQKNLKTLGKWARREGIDCWRVYDADIPEYA
                     LAIDLYGEHAHVQEYEAPKSIDPDKAQARLEQALTVLPQALGLPRERIALKVRRRQRG
                     LEQYEKQAERREYLEVREGEARLWVNLHDYLDTGLFLDHRLTRARVAALARGRDFLNL
                     FAYTGTATVQAALAGARSTTSVDLSATYLDWAQANLELNGLTGPEHRFERADCLAWLE
                     RARGRWGVIFLDPPTFSNSKRMDGTLDVQRDHVPLIRAAAALLEDDGVLVFSNNHRRF
                     RLDTDALAALGLAAEDISAQTIPKDFERNPRIHRCWLIR"
BASE COUNT         1160 a         2747 c         2612 g         1093 t
ORIGIN      
        1 tgttcaccgg catcgagaag ttcgagatca tggcgatccc gttcttcatc ctcgccggca
       61 acttcctgac ccacggcggc gtggcgcgcc ggatgatcaa cttcgcgacc tcgatggtcg
      121 ggcactggca cggcggcctg ggcctggccg gcgtcattgc ctgcgcgctg ttcgcggccg
      181 tgtcgggatc ctcgccggcg accgtggtcg cgatcggctc gatcctgctg ccggcgatgc
      241 tgaagcaggg tttcccgaac cgcttcggtg ccggcgtcat cgccaccagc ggcgcgctcg
      301 gcatcctgat cccgccgtcg atcgtcatgg tcatgtactc ggtcgcgacc aacacctcgg
      361 tcggcgcgct gttcatggcc ggcgtcgtgc cgggcctgat gctcgccggc ttcctggcgt
      421 tcacgacctg gtaccgcgcg aagaaattcg gctatccgcg ccagccgaag gcgacgatgc
      481 aggagcgcat caaggccttc cgcgagtcgg tctggggcct gctgctgatc atcatcgtca
      541 tcggcggcat ctacgccggc ctgttcacgc cgaccgaggc cgcggcgatg agcgccgtct
      601 acgccttcat cgtcgccgtg ttcatctaca aggacatggg cctgaaggac gtgccgaagg
      661 tgctgctcag ttcggcgaac atgtcggcga tgctgctgta catcatcacg aatgccgtga
      721 tgttcagctt catcatgacc gaccagaaga tcccgcagac gctggccgag atcatgatcg
      781 gcagcggcct cggcccgatc accttcctgc tgatgtgcaa catcgtgctg ctgctcgccg
      841 gcaacgtcat ggagccgtcg tcgatcgtgc tgatcttcgc gccgatcctg ttcccgatcg
      901 cgatgcagct cggcatcgac ccggtgcact tcggcatcct gatcgtcgtc aacatggagg
      961 tcggcatgtg tcatccgccg gtcggcctga acctgtacgt ggcctcgggc atcaccaaga
     1021 tgggcatcac cgagctgacc atcgccgtgt ggccgtggct gctgacgatg ctggtcttcc
     1081 tcgtgatcgt gacctacgtg ccggcgatct cgctgacgct gccgaagctg ctcggcgtca
     1141 tgtgatgcgc ggctgatcga cgcaacccct ctgcgaaggc ccggcaggcg aagccgggcc
     1201 ttcggttttt cacgctgcac catccgccgc cggcccggct gcggcgggtc aggcgtcggg
     1261 ttcctgcgtc cgggcatcga ggttgcgccc gatgtgcgca agcgtcgcct cgaaggccgc
     1321 gagcgcttcg gcatcgaggc cggccagcag gcgcgcgacg aaggcatcgc gggccacgcg
     1381 cccggcggca aggcgatcgg ttccggccgc ggtcagcgcc acctcggtca ggcgctggtc
     1441 gtccggatgg ctgcgccggc gcaccagacc ttcggcttcg agcagctgca gctgccgggt
     1501 cagcgccgcg gcatcgacgc cgatcagcgt cttcatgtgc ttctgcgtgc tggccccgtg
     1561 ctcgtgcagg tggttcagga tgcgccagcg cgccagatgc atgccgaccg ccgcctcgaa
     1621 ggccgtcgac agccgctgtg cgagccgcac catgtgctgc agcgcggaag cagactggct
     1681 catgtccccg cctccgccac ccggccgcgc cgccgccagc ggccggcgag cagcgcgatc
     1741 agcaccagcg cggcgagccc ggccgcgagc agcaggccga ggtggatgcc ggccgcgagc
     1801 gcccggtgcg cggcatcgag cagttgcgcg agctgcgccg ggccgtccgg caggcgcgcc
     1861 gccagcgtcg tggcctgatg cagaccggcc ggatcgagca gcagctgcgg atcggcgaac
     1921 agcggccgca gcgcctgcgc gccggcaccg ctgccggcgg cgtccatcgc cgtgcgccag
     1981 gtgccggtga ccagcacgcc gagcacgacc gtgccggcca tgctgccgag cgcgcgcacg
     2041 gtctgcacca gcgccgtcgc cacgccgagt tcagcgcgcg gcacgctggt ctgcaccagc
     2101 agcgtgaagt tcgggaacag gaagccgagt ccgagcccgc acatcaggaa ggcgccggcc
     2161 gcaagccacg ccgaggtctg cgcgccgacc gcctgcacgc cgagcacgcc ggccccgagc
     2221 agcagccagc cgggcagcag cagggattcc ggatcggcga gccgcccgta cagccggccg
     2281 ttgatcaggc tgccgagcgc gatcgccagc accagcggcg tcacgatcag tccggcctcg
     2341 tgcggcgaat ggccataagc gccctgcagc agcagcgggg cgtagaacag cgccgcgaac
     2401 atcacggccc cggccgcgag cgcgcagagc acgacgcggc gcacgcgcgg gatcgcgagc
     2461 agccgcagcg gcagcagcgg atggcgggcg tgcagctgcc ggcgcacgaa cagcagcagc
     2521 gccagcacgc cgccggccag ccacagcggc agcgcaccgg cgaaccacgc gccggcgcgc
     2581 tggccgagtt cgacccacag cagcagcgcc gcaagtccgc cgctcagcca cagcgcaccg
     2641 ggcaggtcga agccggcgcc ctcggcgcgc gtgccgcggc tgcgcggcag ataggcgatg
     2701 accagcacca gcgccagcag cccgagcggc aggttgacgt agaacaccga gcgccagccg
     2761 aagtgctcgg tcagcagccc gccgagcacc ggcccgatgc cgctcgcgag cgcgaagctg
     2821 ctcgacatca gcgcctgcca gcgcagccgg tgcgcggtgt ccgggaacag gtcggcgatg
     2881 ctcgtgaagg cggtggcgat gatcatcgcg ccgccgacgc cctgcagcgc acgcgccgcg
     2941 atcagcgccg gcatcgagcc ggcctgtccg cacagtgccg aggcgccggt gaacacgatg
     3001 atcgccgcga tcacgaaggg cttgcgcccg aacaggtcgc cgagccggcc gagcaccggc
     3061 agcagcgtcg tcgagctcag cagataggcg gtcgcgaccc acgcgaactg ctcgaagccg
     3121 cgcagctcgg cgacgatggt cggcatcgcc gtgccgacca cggtctgatc gagcgcaacc
     3181 agcatcgcga ccagcgcgat gccgagcatc gcgcgcaggg cctgcgcgcc gtacaggccg
     3241 gcaggcgcgg cggtgccggc ggccggcacg ggctcggttc cggcgagggg gacggcggac
     3301 atcggcgacg gactccggcg ggggaaaggc cgccagcatc gcgccggatg attgaccgat
     3361 caatcattga tcgatcaaat caggctcatg gctgccatgc accggtgtgt gcttgtcctg
     3421 ccccggaaac gacgacgccg ccccgggggg cggcgtcggc agcgcacggc aaggcggcgc
     3481 ggggatcagc cccagctgcg caccgggccg gagtcgatgt gcacgaagtt gctgcgcggg
     3541 taatagccga cgccaccggc gcgcaggctc agcgccgcca tgcgcatctg gccgacgccg
     3601 aggccgggca tgcgcacgtc gagcgcgcgc gcctgcatgt ggaagctgtt cttggcgacg
     3661 ccgcgcgatt cctcgcgcag cagcgcgttg gtctcgggcg agcggtagcc ggagatgatg
     3721 tgggtctcgg gccgctgcag catcagctgc atcgcgtaca ggatgtcgag cgcccgcgga
     3781 tcgaacagcc ggaactcgtc gttgtggtga tcgcgcagtg cccaggagat ttccttgatc
     3841 gactgcggca ggtagccggt gttcggcgtc cagaacacct cgcgcacgct ctcgccggtg
     3901 cccggcgcgt acagcgacag cacgcggtcg ccgttgcgga acaggctctc ggtcgacgcg
     3961 gttttcgact cggtgatgat gcccggcgct tcgaggccgc gggtgtcggc cacgatgctg
     4021 ccacggccga tgcgctgctc gccgcgatcg acatagccgc ggccggtgct gcgcagctgc
     4081 ggcttgtagg tgctgccgcc gcggatctgc gcgaccacgc gcccgccgac cggcttgccc
     4141 cgggcatcga cgcggatctt caggttgctc ttgccggccg gcgcctgacg cagcgcgatc
     4201 accggcttcg ccggcatgcg cttgccggtc gcggcacgac cggccacgct ggccccggca
     4261 gccggctttg cggcgcgggc ggacgtgcgg gccgtggacg cagatgcgga ctttgcggtg
     4321 ctgcgggccg tgctgaccgt atgcttgccg ttgccggcgc tgcgggccag caccggcagc
     4381 gcgatcaggc tgctgaccag caggccggcg gtgcgcagga agccgcgccg ctgcgggcag
     4441 ggctcggccg catccagcag cggttgatcg atttcgatca gcggcagcga cacgctcttg
     4501 gaagtggtca tccgtcggaa cctcgaatcg acaaacttta tgagattatt aaaacaaatt
     4561 tacacacaga cgcaaactta aattctcaaa acgacactcg tatcaggttt gcaacaatac
     4621 cgctgctttt ttacaaaatc gtaatccatc aaatacttga ttgagatcat gttcatgagt
     4681 aaggccaaaa cccctgtcac tgccgccatc cgcttgttgc gacagcacaa agtcgggttt
     4741 tcggatcatt tatacgaata tgaagagcgc ggcggcaccg cccacagcgc ccatgcgctg
     4801 gggttgcccg agcacgccgt cgtgaagacg ctgatcatgg aagacgaccg gcatgaaccc
     4861 ctgatcgtgc tgatgcacgg cgacctgcag gtgtcgacga aggagctcgc gcgccagatc
     4921 ggcgcccgct cgatcgcccc ctgcgcaccc gaggtcgcca accgccacag cggctatctg
     4981 gtcggcggca cctcgccctt cggcacgcgc cacgcgatgc cggtctacgt cgaggcgacg
     5041 atcctcgaac tgccgcgcat ctggatcaac ggcggcaagc gtggctacct gatcggcatc
     5101 gagccgtcgg tcttgagcac ggtgctgaaa ccgctgccgg tgcgggtcgg catccccggc
     5161 tgaggtgcgc atcggttccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 gcgatgttcg acggtgccat cgcctgcagc tgccggcgca gatcgccgac cagccggcgc
     5341 agcgcgtcga gctcgcgccc ctggcggtag acgaggtcgc tgagttcatt gagcgcggcg
     5401 tcctgatgcg cgtagcgaat ctccagatcg atcaggcgct gttcgtccac ggcggcgggc
     5461 tccgggtacg ggtcgggggg cgcgaattct agccgccgcg cggccggacg ctagaatgcc
     5521 ggcccgcgcc tgcccggtac ccgcgccgat gtctgccgat cttccgtttt tcgcgaccgc
     5581 cccgcgcggc gtcgaaccgc tgctcgccgc cgaactcgcc gcgctcggcg ccgcgccggt
     5641 gcgcgagggc cgcggcggcg tcagcttcgg cggcccgctc gaaaccgctt accgcgtctg
     5701 cctgtggtcg cgcaccgcga gccgcgtgct gcggctgctc gcgcgcgtgc cggccgccga
     5761 tgccgatgcg ctgtacgccg gcgtgctcga cctgccgtgg gaggatcacc tcgaccccga
     5821 cggcacgctc gcggtcgatt tcgtcgccgc cggccgcagc gccatccgcc acacgcagtt
     5881 cggcgcgcaa cgcgtcaagg atgccgtctg cgaccggctg cgcgagcgct gcggccggcg
     5941 gccctcggtc gatcgcgccg cgccggacct gcgcatcaac gtgcatgcca gcggcgagac
     6001 ggccgatgtc gcgctcgatc tctccggcga atccctgcac cggcgcggct accgcgagga
     6061 cggcgcgctg gcgccgctga aggagaacct cgccgccgcg ctgctgctga aggccggctg
     6121 gccggcgatc gtcgaagccg gcggcgcgct gctcgacccg atgtgcggct ccggcacctt
     6181 cgtcatcgag gccgcgctga tcgccgccga ccgcgccccc ggcctgctgc gcccggactg
     6241 gggcttcttc ggctggcgcg gccacgacgc cggcctctgg cgccagctcg tgctcgaagc
     6301 ccgcgagcgc gccgccgccg gcctcgcgaa accgctgccg tcgctggtcg gcagcgatca
     6361 cgaggcccgc gcgatccgcg ccgcgctgac caacgccggt caggccgaaa tcgcgaagct
     6421 cgtgcacttc gagcgccgcg aactcgccgg cgccgtgcca ccgaagggcg cgcccggcct
     6481 cgtcatcgtc aatccgccct acggcgagcg cctcggcgaa ctcgaagccc tcgccgacac
     6541 ctacgccgcg ctcggcgacg tgctgaaggc gcgcttcggc ggctggcagg cggcggtgtt
     6601 caccggcaac cccgatctcg gcaagcgcat gggcctgcgc gccggcaagg tgcatgcgtt
     6661 ctacaacggc ccgatcgaat gcaggctgct gcgcttcgag gtcacgcccg agcagcacgt
     6721 cgatcgcgag gccgtcgacc gccgccgcgc cgggcaggcg atcgaacgcg cgctcgccgc
     6781 cggcgccgat gcgctggtca accgcgtgca gaagaacctg aagacgctcg gcaagtgggc
     6841 gcggcgcgag ggcattgact gctggcgcgt ctacgacgcc gacatccccg aatacgcgct
     6901 ggcgatcgac ctctacggcg agcacgcgca cgtgcaggaa tacgaggcgc caaagtcgat
     6961 cgaccccgac aaggcgcagg cccgcctcga acaggcgctg accgtgctgc cgcaggcgct
     7021 cggcctgccg cgcgagcgca tcgcgctgaa ggtccgccgc cgccagcgcg gcctggagca
     7081 gtacgagaaa caggccgagc gccgcgaata cctcgaagtg cgcgaaggcg aggcgcggct
     7141 gtgggtgaac ctgcacgact acctcgacac cggcctgttc ctcgaccacc ggctgacgcg
     7201 cgcgcgggtc gcggcgctgg cccgcggccg cgacttcctg aacctgttcg cctacaccgg
     7261 caccgccacc gtgcaggccg cgctcgccgg cgcgcgctcg accaccagcg tcgacctgtc
     7321 ggcgacctat ctcgactggg cgcaggccaa tctggagctg aacggcctga ccggccccga
     7381 acaccgcttc gagcgcgccg actgcctcgc ctggctcgaa cgcgcgcgcg gacgctgggg
     7441 cgtgatcttc ctcgatccgc cgaccttctc gaactccaag cgcatggacg gcacgctcga
     7501 cgtacagcgc gaccacgtgc cgctgatccg cgccgccgcg gcgctgctcg aggacgacgg
     7561 cgtgctggtg ttctcgaaca accaccggcg cttccgcctc gacaccgacg ccctcgccgc
     7621 cctcggcctc gcggccgagg acatcagcgc gcagaccatc ccgaaggact tcgaacgcaa
     7681 cccgcgcatc caccgctgct ggctgatccg gc
//
LOCUS       DAOQIE010000191         7705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4931_length_7705_cov_7.748889, whole genome shotgun sequence.
ACCESSION   DAOQIE010000191 DAOQIE010000000
VERSION     DAOQIE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_7705_cov_7.748889"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK144_12085"
     CDS             <1..345
                     /locus_tag="PK144_12085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF66516.1"
                     /translation="HVEYRHGLRQALQGSLLTLGAALLTGDLANAGSFIAHLPVVLAG
                     LHYSRDFEREADARGAALMRAEGRDPRALARALARLEGAARQKERPAAPAWLSDHPDT
                     QERIARIEALAR"
     gene            474..917
                     /locus_tag="PK144_12090"
     CDS             474..917
                     /locus_tag="PK144_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF66517.1"
                     /translation="MPLPVTPRLTVDAIIELADRPGRPIVLIERRNPPPGWALPGGFV
                     DVGERVEDAAIREAAEETGLDVRLITLLGIYSDPARDARFHTAGVVYVAEATGTPVAA
                     DDARHLDVFDPDGPLPLLAFDHAQILADYRQWRAHGTGAPLRPAH"
     gene            1108..1689
                     /locus_tag="PK144_12095"
     CDS             1108..1689
                     /locus_tag="PK144_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF66518.1"
                     /translation="MSAPAELTDLLLASGRGDRPAFERLYRLSSPRLYSLARWLLKRD
                     ALAEEIMQEAYVQIWRDAARFDPARASAMTWMAVIVRHRALDELRRKRPELEPGGGSA
                     GSDEDDAMPEPGVAEADPDAGPLELALADGDRRQLGICLETLSEMQRQSILLAFLRGL
                     THSEVASALAAPLGSVKSWVRRGLEQLKRCLTS"
     gene            1686..2408
                     /locus_tag="PK144_12100"
     CDS             1686..2408
                     /locus_tag="PK144_12100"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNF66519.1"
                     /translation="MNYRDPKLGTRLAAEYALGTMGGLARRRFEGLMAADPWLRREVR
                     AWQDDLYGIVELIPERTPPERLWTHIETRLWPVATGRVVPLAPKARPSRLWQVWSGLA
                     TAAALLLAVAVWRAPAPPSPQPLAVPDYVAVVSDRDGRPAWLVRPAPGGGLAVSALAP
                     QALASDRAFELWLLPPGGAPPKSLGLLPADGGARLGVAPERLREIVEGDGLAVSLEPA
                     GGSPTGAPTGPVLYLGKPLPRV"
     gene            complement(2475..3206)
                     /locus_tag="PK144_12105"
     CDS             complement(2475..3206)
                     /locus_tag="PK144_12105"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF66520.1"
                     /translation="MKATLFVADLHLDAAARPQTLALFEDFIAVRARRAQALYILGDL
                     FEAWIGDDDDGETGERVAAALGSLQRSGVPAHFLHGNRDFLLGPDYAARAGLTLLPET
                     TVIDLYGTPTLLTHGDALCTDDVDYQRFRAQIREPANQARLLALPLAQRRAFAAQLRA
                     DSRSAQADKPAEITDVNPDAVRTLMDAHGVTQLIHGHTHRPALHELVLADGDSGWRVV
                     LGDWYTQGSVLAVSRNGMQLEGLHG"
     gene            complement(3203..3691)
                     /locus_tag="PK144_12110"
     CDS             complement(3203..3691)
                     /locus_tag="PK144_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF66521.1"
                     /translation="MIRIQTSLGVITLELDAEHAPATTANFEQYVRDGFYAGTIFHRV
                     IPNFMVQGGGMLADMSQKSTRAPIRNEAKNGLKNARGSIAMARTSDPHSATAQFFINV
                     KDNGFLDHPGQDGWGYCVFGKVIEGMDVVDAIVAAPTANKRGHQNVPVEPVLIEKMEI
                     VE"
     gene            complement(3742..4242)
                     /locus_tag="PK144_12115"
     CDS             complement(3742..4242)
                     /locus_tag="PK144_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF66522.1"
                     /translation="MAVTRVQMVTSHGTIVLELDEEKAPQTVANFVRYADEHFYDGTI
                     FHRVIDNFMIQGGGFTPDLQQKPTHDPIPNEAKNGLKNSRGTIAMARTADPHSATAQF
                     FINVRDNGFLDYPSRDGWGYCVFGKVVDGLEVVERIRQTPTTTVGPYSDVPAEPVVIR
                     SVTVVK"
     gene            4460..5905
                     /gene="cysS"
                     /locus_tag="PK144_12120"
     CDS             4460..5905
                     /gene="cysS"
                     /locus_tag="PK144_12120"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNF66523.1"
                     /translation="MSVEHDLQLYNSLSRRKERFVPITPGQVRMYVCGMTVYDYCHVG
                     HARVMVVFDAIVRYLRWLGYDVTYVRNITDIDDKIIRRAQENGETIDALTARFIEAMH
                     EDADALGVLRPDHEPKATETIDGIIAMVGTLVGKDYAYVAANGDVYYRVARFGAYGRL
                     ANKKLDDLRAGERVAVGELKQDPLDFVLWKAAKPGEPAWDSPWGPGRPGWHIECSAMS
                     KCCLGEHFDIHGGGMDLKFPHHENEIAQSEAANGHRFVNYWLHNGFVNVDEEKMSKSL
                     GNFFTVREVLARYAPEVVRLFILSSHYRSPLNYSDDNLDTAKASLTRLYTALKGLPPT
                     AAAPLEAWSARLRAVMDDDFNTPGALAVLFELARELNRARSEAPADVDALAATLRALG
                     GVLGLLGGDADDFLKGYLTFQISTRTEDVKTSIEFFVGLSVDEIERLIAERTAARKAK
                     QWAESDRIRDLLAAQGVVLEDGPQGTSWRRA"
     gene            5998..6074
                     /locus_tag="PK144_12125"
     tRNA            5998..6074
                     /locus_tag="PK144_12125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6032..6034,aa:Pro,seq:tgg)
     gene            6107..6183
                     /locus_tag="PK144_12130"
     tRNA            6107..6183
                     /locus_tag="PK144_12130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Arg,seq:tct)
     gene            6215..6290
                     /locus_tag="PK144_12135"
     tRNA            6215..6290
                     /locus_tag="PK144_12135"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6248..6250,aa:His,seq:gtg)
     gene            6336..6411
                     /locus_tag="PK144_12140"
     tRNA            6336..6411
                     /locus_tag="PK144_12140"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6369..6371,aa:Lys,seq:ctt)
     gene            7144..>7705
                     /locus_tag="PK144_12145"
     CDS             7144..>7705
                     /locus_tag="PK144_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HNF66524.1"
                     /translation="MLKTRTFFLGTLAGGLALALAGSSAHAADTIKVGILHSLSGTMA
                     ISETSLKDVALMTIEEINAKGGLLGKKLEAVVVDPASNWPLFAEKARELLQKEKVAVT
                     FGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEESSYNVFYTGAAPNQQAIPAVEYLMS
                     EDGGAAKRWVLLGTDYVYPRTTNKILR"
BASE COUNT         1159 a         2563 c         2665 g         1318 t
ORIGIN      
        1 cacgtcgagt accggcacgg cctgcgccag gcgctgcagg gcagcctgct gacgctcggt
       61 gcggcgctgc tgaccgggga tcttgccaat gccggcagct tcatcgcgca tctgccggtc
      121 gtgctcgccg gcctgcatta ctcgcgcgac ttcgagcgcg aggccgatgc gcgcggtgcg
      181 gcgctgatgc gcgccgaagg ccgcgatccg cgcgcgctcg cccgggcgct ggcgcggctc
      241 gaaggcgcgg cccggcagaa ggaacgtccg gcagcgccgg cctggctgtc cgatcacccg
      301 gacacgcagg agcgcatcgc gcgcatcgag gcgctggcac gctgaagcct gcgccgcacc
      361 ggccgcgagg ggcgccagcg ggcaggctcc ggcacgagtc cggcttgccc gggcgcgtgc
      421 agccgctacg gtttggatga atcttcccct ggccgccccc cggagcctgc ccgatgcccc
      481 tgcccgtcac gccgcgcctg accgtcgatg ccatcatcga actcgccgac cgccccggcc
      541 ggccgatcgt gctgatcgag cggcgcaacc cgccgccggg ctgggcgctg cccggcggct
      601 tcgtcgatgt cggcgagcgc gtcgaggacg ccgcgatccg cgaggcggcc gaggagaccg
      661 ggctggacgt gcgcctgatc acgctgctcg gcatctactc ggacccggcg cgcgacgcgc
      721 gcttccacac agccggagtc gtctatgtgg ccgaggccac cggcacgccg gtggctgccg
      781 atgacgcgcg ccacctcgat gtcttcgatc cggacggccc gctgccgctg ctcgccttcg
      841 atcatgcgca gatcctcgcc gattaccgcc agtggcgggc gcacggcact ggcgcgccgc
      901 tgcgcccggc gcactgaatt cccgttctca cggcagcgga tgccatcctg atttcatcct
      961 gattccgtcc ggattccatt ctgactgcat ccgttcgccg gtccggcccg catggcttgc
     1021 tgacggcgat gctgcgcgac caccgctgcc gcccgaatgc acgggtgggc agcagggcgg
     1081 tggcgcctga tcacacgggg agtctccatg tctgcacccg ccgaattgac cgatctgctg
     1141 ctggccagcg gccgtggcga tcgacctgcc ttcgagcgcc tgtaccggct cagctcgccg
     1201 cgtctgtact cgctggcgcg ctggctgctc aagcgcgatg cgctggccga ggagatcatg
     1261 caggaggcct acgtgcagat ctggcgcgac gccgcccgct tcgatccggc gcgcgcgtcg
     1321 gcgatgacct ggatggcggt gatcgtgcgc caccgcgcgc tcgacgaact gcgccgcaag
     1381 cgtccggagc tcgaacccgg cggtggttcc gccggcagcg acgaggacga tgccatgccc
     1441 gaaccgggag tggccgaggc cgatccggat gccggcccgc tggaactcgc gctcgccgac
     1501 ggcgaccgcc gccagctcgg catctgtctg gagacgctga gcgagatgca gcgccagagc
     1561 atcctgctcg ccttcctgcg cggcctcacg cactccgagg tcgccagcgc gctggccgcg
     1621 ccgctcggca gcgtcaagag ctgggtgcgc cgcggactcg aacaactgaa gaggtgcctc
     1681 acgtcatgaa ctaccgcgat ccgaaactgg gcacccggct cgcggcggag tacgcgctgg
     1741 gcacgatggg agggctggcc cgccgccgtt tcgagggttt gatggccgcc gatccctggc
     1801 tgcggcgcga ggtgcgcgcc tggcaggacg atctctacgg catcgtcgag ctgatcccgg
     1861 agcgcacccc gcccgagcgg ctgtggacgc acatcgaaac ccggctgtgg ccggttgcga
     1921 ccggcagggt cgtgccgctc gcgccgaagg cccggccgtc gcggctctgg caggtctgga
     1981 gcggactggc gacggcggcg gcattgctgc tggccgtggc cgtgtggcgg gcgccggcgc
     2041 cgccctcgcc gcagccgctg gcggtgcccg attacgtggc cgtggtcagt gaccgcgacg
     2101 gccggccggc ctggctggtg cgcccggcgc cgggcggcgg actcgcggtc agcgcgctgg
     2161 caccgcaggc gctggccagc gaccgcgcct tcgaactctg gctgctgccc ccgggcggtg
     2221 cgccgccgaa gtcgctcggc ctgctgccgg ccgacggcgg cgcgcgcctc ggcgtggcgc
     2281 cggagcggct gcgcgagatc gtcgagggcg acggcctcgc cgtcagcctg gagccggccg
     2341 gcggctcgcc gaccggtgcc ccgaccggcc cggtgctgta cctcggcaag ccgctgccgc
     2401 gcgtctgacg cgctcgtgcg cgcctgcgcg gcccggcggg gcgcgcaggc ggggcagggc
     2461 gccgaggccg gatttcagcc gtgcaggcct tcgagctgca tgccgttgcg cgagaccgcc
     2521 agcacgctgc cctgcgtgta ccagtcaccg agcacgacgc gccagccgct gtcgccatcg
     2581 gcgagcacga gttcgtgcag ggcggggcgg tgggtgtggc cgtggatcag ctgggtgacg
     2641 ccgtgtgcgt ccatgagcgt gcgcacggca tcggggttta cgtcggtgat ctcggccggc
     2701 ttgtcggcct gggcgctgcg actgtcggcg cgcagctgcg cggcgaaggc ccggcgctgc
     2761 gcgagcggca gcgccagcag gcgagcctga ttggccggtt cgcggatctg tgcgcggaag
     2821 cgctggtaat cgacatcgtc ggtgcagagc gcatcgccgt gcgtgagcag cgtcggcgtg
     2881 ccgtacaggt cgatcaccgt ggtctccggc agcagggtca gtccggcgcg tgccgcatag
     2941 tccgggccga gcaggaaatc gcggttgccg tgcaggaagt gcgcgggcac gccgctgcgc
     3001 tgcaggctgc ccagcgccgc ggccacccgc tcgccggttt cgccgtcgtc gtcgtcgccg
     3061 atccaggcct cgaacaggtc gccgaggatg tacagcgcct gcgcgcgccg ggcgcggacg
     3121 gcgatgaaat cctcgaacag cgccagcgtc tgcgggcgcg cggcagcatc gaggtgcagg
     3181 tcggcgacga acagcgtcgc cttcattcga cgatttccat cttctcgatc agcaccggct
     3241 cgaccggcac gttctggtgt ccgcgcttgt tcgcggtcgg cgcggcgacg atcgcatcga
     3301 ccacgtccat gccctcgatg accttgccga acacacagta cccccagccg tcctgcccgg
     3361 ggtgatcgag gaagccgttg tccttgacgt tgatgaagaa ctgcgcggtc gccgagtgcg
     3421 gatccgaggt gcgcgccatc gcaatgctgc cgcgggcgtt cttcaggccg ttcttcgcct
     3481 cgttgcggat cggcgcgcgc gtgctcttct ggctcatgtc cgccagcatg ccgccgccct
     3541 ggaccatgaa gttcgggatc acgcgatgga agatggtgcc ggcgtagaaa ccgtcgcgca
     3601 cgtactgttc gaagttggcg gtggtcgccg gcgcatgctc ggcgtcgagt tccagcgtga
     3661 tgacgccgag tgaggtctgg atgcggatca tcgcgaatct ccgttcgggg tttccggggg
     3721 cgggcctgct ttatctcatg actacttcac gaccgtgacc gagcggatca ccaccggctc
     3781 ggccggcacg tcgctgtatg gtccgaccgt ggtggtcggt gtctggcgga tgcgctcgac
     3841 gacttcgagg ccgtcgacga ccttgccgaa cacgcagtaa ccccagccgt cacgcgacgg
     3901 gtaatcgagg aagccgttgt ccctgacgtt gatgaagaac tgcgcggtcg ccgagtgcgg
     3961 atcggcggtg cgtgccatcg cgatcgtgcc acggctgttc ttcaggccgt tctttgcttc
     4021 gttcgggatc gggtcgtgcg tcggcttctg ttgcaggtcg ggcgtgaagc cgccgccctg
     4081 gatcatgaag ttgtcgatga cgcggtggaa gatcgtgccg tcgtagaagt gctcgtccgc
     4141 gtagcgcacg aagttggcga cggtctgcgg ggctttttcc tcgtcgagtt cgagcacgat
     4201 cgtgccgtga ctggtgacca tctgcacgcg ggtcaccgcc atggccggca gggtggccga
     4261 aagcagggcg gcgatcagga aggctcgggc gcaacggatc atcggcagcc tccgggggcg
     4321 ggcgcggaaa ggccggcatc atagtgtgcc gcccggcttt tgtcggcctg cacgcgccgg
     4381 acttccgtta ccatcccggt cgggccctgc tgccggggcg ccgtccgtcc gttttccgtc
     4441 gcatcgagtc cagtccgtca tgtccgtcga acacgatctg cagctgtaca acagcctgag
     4501 ccggcgcaag gagcgcttcg tcccgatcac gcccggccag gtgcgcatgt atgtgtgcgg
     4561 catgaccgtc tacgactact gccacgtcgg ccacgcgcgc gtgatggtcg tgttcgatgc
     4621 gatcgtgcgc tacctgcgct ggctcggcta cgacgtgacc tacgtccgca acatcaccga
     4681 catcgacgac aagatcatcc gccgcgcgca ggaaaacggc gaaaccatcg acgcgctgac
     4741 cgcgcgcttc atcgaggcga tgcacgagga tgccgatgcg ctcggcgtgc tgcgccccga
     4801 ccacgagccg aaggccaccg aaaccatcga cggcatcatc gcgatggtcg ggacgctggt
     4861 cggaaaggac tacgcctacg tcgctgccaa cggcgatgtc tactaccgtg tcgcgcgctt
     4921 cggggcctac ggccggctcg ccaacaagaa gctcgacgac ctgcgcgccg gcgagcgcgt
     4981 ggcggtcggc gaactgaagc aggacccgct cgacttcgtg ctgtggaagg ccgccaagcc
     5041 cggcgagccg gcctgggatt cgccgtgggg accgggccgg ccgggctggc acatcgaatg
     5101 ctcggccatg tcgaagtgct gcctcggcga gcacttcgac atccacggcg gcggcatgga
     5161 tttgaagttc ccgcaccacg agaacgagat cgcgcagagt gaggccgcca acggccacag
     5221 gttcgtcaac tactggctgc acaacggctt cgtcaacgtc gacgaggaga agatgtcgaa
     5281 gtcgctcggc aacttcttca cggtgcgcga ggtgctcgcc cgctacgccc ccgaggtcgt
     5341 gcggctgttc atcctgtcga gtcactaccg cagcccgctg aactactccg acgacaacct
     5401 cgacacggcg aaggcctcgc tgacgcggct gtacacggcg ctgaagggcc tgccgcctac
     5461 cgccgccgcc ccgctcgaag cctggagcgc aaggctgcgc gcggtcatgg acgatgactt
     5521 caacaccccc ggggcgctcg ccgtgttgtt cgagctggcg cgcgagctga accgtgcgcg
     5581 cagcgaggcg ccggccgatg tggatgcgct ggcggcgacg ctgcgcgcgc tgggcggcgt
     5641 gctcggcctg ctcggcggcg atgccgacga ttttcttaag ggatacttga cttttcagat
     5701 ttctacgaga actgaagatg taaagacatc gatcgagttc tttgtgggat tgtcagtaga
     5761 cgaaatcgag agattgatcg ccgagcgcac cgctgcgcgc aaggcgaagc agtgggccga
     5821 gtccgaccgc atccgcgatc tgctggccgc gcagggcgtg gtgctggagg acggtccgca
     5881 gggcacgagc tggcggcggg cctgagtcag gcgtcagacc gaggcttgac gcgaaatcgt
     5941 cgatccgtag aatgacgggc cttgcacgca ccctgttgca agcgctggct gactcggcgg
     6001 ggtatagcgc agtctggtag cgcacctgct ttgggagcag ggtgtcgggg gttcaaatcc
     6061 ctctaccccg accatccagc ccggcgcgcc ggtcctgacg aacgccgcgc ccgtagctca
     6121 tgcggataga gcatcggcct tctaagccga gggtagcagg ttcgaatcct gccgggcgcg
     6181 ccactcccgg cagtcgtgca atcaggtttc agtggtggct gtagctcagt tggtagagcc
     6241 ccggattgtg attccggttg tcgtgggttc gagccccatc agtcacccca tcagttcatt
     6301 ccggaccgcg ctgcggtccg gatgcccgaa aaaacgggcc gttagctcaa ttggtagagc
     6361 agttgactct taatcaattg gttgtaggtt cgagtcctac acggcccacc atcttcagcg
     6421 aatgaaaatg atatcaaagg gttgcgggca aaccgcaacc ctttttcttt tctggcccga
     6481 tctccttccg tccattctcc gtcgacgttt tcaccggcgt caggcgcgta tcgtgacgca
     6541 tgtcctccct tcgtccgtct gctgcggtga acgggggcgg gcacgcgcaa ggcctggatt
     6601 ctccctcaga ccccgaggca gttcaggcca agggtgagcg gtccggcgcc agtcttgctt
     6661 ccggcgccgg ccagttgtcc ggcgatcatc cagcctgtct ccgggcgacg atcgggcgat
     6721 tcgcaggacg ggcgtcacgg ctgcgtctgc aatccgcggg cttcgggctt cagtcccgtg
     6781 cgccggtcga tgccggcggt tcgggatggt gatgctgata tccactgagc tttctgaaat
     6841 ttaagttgcc tgatttcaat aatggtgcat cgccgtaagg aggcatattg cggcgatggt
     6901 gcagccgaag gcgcatgcgc accactttgg ttcatattga tttcagtgat gcacgattgt
     6961 ggtgcgtaag cttcgctcta cagcagtcgt caacatgatg cgttgccgca aagccatgaa
     7021 ccggcagtga agccggcctt tggcatggcg aatgctcgaa aggttcctgc gtccaggggg
     7081 ggcattccgt tcggagtgcc cgcgagggga cgaccacaaa cagaaccgta tcgaggattt
     7141 tccatgctca agacccgcac cttcttcctg ggcacgctgg ccggtggtct cgcgctcgcc
     7201 ctggctggca gcagcgcgca tgcggccgac accatcaagg tcggcatcct gcattcgctg
     7261 tccggcacga tggccatctc ggaaacgtcg ctgaaggacg tggcgctgat gaccatcgag
     7321 gaaatcaacg ccaagggcgg cctgctcggc aagaagctcg aagccgtcgt cgtcgatccg
     7381 gcctcgaact ggccgctgtt cgccgagaag gcgcgcgagc tgctgcagaa ggagaaggtt
     7441 gcggtcacgt tcggctgctg gacctcggtg tcgcgcaagt ccgtgctgcc ggtgttcgag
     7501 gaactgaacg gcctgctgtt ctacccggtg cagtacgaag gcgaggagtc ctcgtacaac
     7561 gtcttctaca ccggtgccgc gccgaaccag caggcgatcc cggccgtcga gtacctgatg
     7621 tccgaggacg gcggcgctgc caagcgctgg gtgctgctcg gcaccgacta cgtgtacccg
     7681 cgcaccacca acaagatcct gcgcg
//
LOCUS       DAOQIE010000192         7675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_4961_length_7675_cov_7.524409, whole genome shotgun sequence.
ACCESSION   DAOQIE010000192 DAOQIE010000000
VERSION     DAOQIE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4961_length_7675_cov_7.524409"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="hemA"
                     /locus_tag="PK144_12150"
     CDS             complement(<1..624)
                     /gene="hemA"
                     /locus_tag="PK144_12150"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNF66525.1"
                     /translation="MPLLAIGINHKTAPVSVRERVAFAPERLRQALVDLVAAGAARGA
                     AILSTCNRTEVYCDSASAEAEQVCAWLSHWHAIPAQELRPYLYVHPGQEAVRHMLRVA
                     SGLDSLVLGEPQILGQVKDAYQSAADAGTLDGELDRLFQHTFAVAKQVRTDTSIGASP
                     VSVAFAAVALARQIFGKLGKRTALLLGAGETIELVARHLREQDIQRLI"
     gene            739..2463
                     /locus_tag="PK144_12155"
     CDS             739..2463
                     /locus_tag="PK144_12155"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF66526.1"
                     /translation="MKPAATAALALALSLALVPVTRADPAAPAAPGPAPADPAPPPST
                     AAARDTEVWYHILLGELAARRGRFDVAVSEYQAAAAASTDVRVTERAFAVALYARDPA
                     AAAGIARRWQTLLPEAIPPRQALALALIEGGDVESALEPLAWLREHFAVQDQQDGFAA
                     VANLLGQARERQAAWQALARLSAQQPDSRFGHYYAAGAALAAGEGIAAERELQTALKL
                     APDWAPAWQMRARLVLDRGDLPAALEVLSAAVTQLPKDTALRTAYARALFLANRYDEA
                     GEQFDRLLADNPRDSNARLALAGIAFEAKQYDRAVALLKDLLGSGENPGDIEFQLGRV
                     EEARGDYAAARAWYEKVTDGDRRLGARIRLGALKLAEGDEPGMHAWFNAERAAHPDDA
                     VALFIGEAEVLRQKDLIQPAWAVLTAALAGHPDDTDLLYARALVAERLDRIGETESDL
                     RRVLELDPEHGQALNALGYTLADRTDRLAEAEDLIRRALEKLPSDAAVLDSMGWVLYR
                     QGKAAEGLPFLERAQGLSLEPEILAHLVEVLWTLGRQDEARALWERGAQRAPGDRFLQ
                     QVREKLKW"
     gene            2457..3071
                     /gene="lolB"
                     /locus_tag="PK144_12160"
     CDS             2457..3071
                     /gene="lolB"
                     /locus_tag="PK144_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNF66527.1"
                     /translation="MVRRSRLWPAAPAALLALALAGCAELPASRGDATQDPRWQARSA
                     QLAKLRDWQLDARIGVNRDRQAWSASLKWQQHGPRYEIDLLGPFGAGRVRVEGDAAGV
                     VLLRGDDEPPVSARDPDELFERATGIAVPAGALGDWLFGRPQPGVAAQVEADAEGRTT
                     RIVQAGWTIEYPAWQPVGALELPAKLRAVRGDVQVRLAVERWGL"
     gene            3083..3982
                     /gene="ispE"
                     /locus_tag="PK144_12165"
     CDS             3083..3982
                     /gene="ispE"
                     /locus_tag="PK144_12165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNF66528.1"
                     /translation="MSDAGSSPSSPSSSPAFSAGWPAPAKLNRFLHIVGRRADGYHLL
                     QTVFQFIDRADTLAFALRDDGRIERETDLPGVPAEADLVVRAARRLQAETGTRSGVSI
                     RIDKQLPMGGGLGGGSSDAATVLVALNHLWGCGLDDDALAGLGLTLGADVPVFVRGRA
                     AWAEGIGERLTPVEPDEPWFLVLVPAVHVATAAIFKAPELTRDSPLITMRAFLGGAGH
                     NDCEAVVHARHPEVAAATAWLSQHTTARLTGTGACVFGAFADEAAARTVMAGRPAGLE
                     AFVARGRNRSPLLDRLARARAGD"
     gene            4040..4114
                     /locus_tag="PK144_12170"
     tRNA            4040..4114
                     /locus_tag="PK144_12170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4072..4074,aa:Gln,seq:ttg)
     gene            4163..5101
                     /locus_tag="PK144_12175"
     CDS             4163..5101
                     /locus_tag="PK144_12175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNF66529.1"
                     /translation="MMVFTGRAHPELAQRIVDHLGLPMGKAVTGSFSDGECMVELMEN
                     VRGRDVFIVQPTSAPTNDNLMELLVMVDALRRSSAIRITAVIPYFGYSRQDRRPRSAR
                     VPISAKVVANMITTVGVDRVLTIDLHADQIQGFFDLPVDNIYATPIMLEDLFQLHLPN
                     LVVVSPDVGGVVRARALAKRLHDADLAIIDKRRPKANVSQVMHIIGEVEGRDCILIDD
                     MVDTAGTLCHAAKALKDDGAASVRAYCTHAVLSGKAIDNLSNSALDELVVTDTIPLRP
                     EAKACGRIRQLSVAGLLAETIHRIHLEESVSSLFGE"
     gene            5204..5821
                     /locus_tag="PK144_12180"
     CDS             5204..5821
                     /locus_tag="PK144_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNF66530.1"
                     /translation="MSVVAFELNAETRSEQGKGASRRLRIAGRVPAVLYGAGKAPELL
                     SVSQRDILLQLENEAFYSHVLDLKIGDRAERVVLRDLQRHPYKPMVLHLDLQRVDATQ
                     QITVHVPLHFVNQEAAIGVKQQGGVVSHHLSEIEVTCLPDQIPEFIEVDLIALELGQA
                     LHVSQLVLPAGCTAYALTHGQHSDEIVVSIHAARAGGDEEAAPAA"
     gene            5843..6430
                     /gene="pth"
                     /locus_tag="PK144_12185"
     CDS             5843..6430
                     /gene="pth"
                     /locus_tag="PK144_12185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNF66531.1"
                     /translation="MAEALKLIAGLGNPGPAYERTRHNAGFWFVDELARSHGGSFRHD
                     PKLNADTCQITVEGRALWLMKPMGFMNRSGQPLARLANFYKIARPSILVVHDELDLPP
                     GVARLKRGGGHGGHNGLRDIIAQCGGNDFLRLRLGIGHPGTAAEVVDYVLRKAPSSEQ
                     QLIERALDDALRVLPLVVRDEFERAMQTLHTRAPA"
     gene            6471..7562
                     /gene="ychF"
                     /locus_tag="PK144_12190"
     CDS             6471..7562
                     /gene="ychF"
                     /locus_tag="PK144_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNF66532.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIQAENYPFCTIDPNVGVV
                     PMPDARLDALAKIVVPQRILPTTMEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFENDDVIHVAGKVDPLSDIEVINTELALADLASVEKAFQRAEKAAKAGGKDAVA
                     RKELLARVQAHLNEAKPVRAMNLSDDELALLYELHLLTVKPTMYIANVHEDGFDHNPL
                     LDKVRALAAAEGAEVVPVCAAIEAEIAQLDDADKLEFLADLGLDEPGLNRVIRAGYKL
                     LGLQTYFTAGVKEVRAWTVPIGATAPQAAGVIHTDFERGFIRAEVIAYDDFIAGNGED
                     GAKKAGKWRLEGKDYVVQDGDVMHFRFNV"
BASE COUNT         1147 a         2793 c         2602 g         1133 t
ORIGIN      
        1 gatcaggcgc tggatgtcct gctcgcgcag gtggcgggcg acgagttcga tggtttcgcc
       61 ggccccgagc agcagcgcgg tgcgcttgcc gagcttgccg aagatctgcc gggccagcgc
      121 cacggcggcg aaggccaccg acaccgggct cgcgccgatg ctggtgtcgg tgcgcacctg
      181 cttggcgacc gcgaaggtgt gctggaacag gcggtcgagc tcgccgtcga gcgtgccggc
      241 atcggcggcg ctctggtagg catccttgac ctgaccgagg atctgcggct cgccgagcac
      301 cagcgaatcg agccccgagg ccacgcgcag catgtggcgc acggcctcct gacccggatg
      361 cacgtacagg taggggcgca gctcctgcgc cgggatcgca tgccagtgcg acagccacgc
      421 gcagacctgc tcggcctcgg cactggcgct gtcgcagtag acctcggtgc ggttgcaggt
      481 cgacaggatc gccgccccgc gggcggcacc ggccgcgacg agatcgacga gcgcctgtcg
      541 cagccgctcg ggcgcgaaag ccacgcgctc gcggacgctg accggggccg tcttgtgatt
      601 gatgcctatg gccagcaggg gcatgatagg taaaccacct tcacgaaacc gcgggattct
      661 gcgggatggc ccactgcaca cgcaagttgc ggtcactcct gcgtctcgtg cgctcccgcc
      721 ccggtaccgt cgcctccgat gaagcctgcc gctaccgctg cgcttgccct ggcgctcagt
      781 ctcgccctcg tgcccgtcac ccgcgccgat cccgctgccc cggctgctcc cgggccggcg
      841 ccggccgatc cggctccgcc gccgtccacg gccgcggcgc gcgacaccga ggtctggtat
      901 cacatcctgc tcggcgagct cgccgcccgg cgcggccgct tcgatgtggc cgtcagcgaa
      961 taccaggccg cggccgccgc gagcaccgac gtgcgcgtca ccgagcgcgc gttcgcggtc
     1021 gcgctgtacg cgcgtgaccc ggcggccgcg gccggcatcg cccggcgctg gcagacgctg
     1081 ctgcccgagg cgatcccgcc gcgccaggcg ctggcgctgg cgctgatcga gggcggggat
     1141 gtcgagtccg cgctcgaacc gctggcgtgg ctgcgcgagc acttcgccgt gcaggaccag
     1201 caggatggtt tcgccgccgt cgccaacctg ctcggacagg cccgcgagcg gcaggctgcc
     1261 tggcaggcgc tggcacggct gagtgcgcag cagcccgatt cgcgcttcgg tcactactac
     1321 gccgccggtg ccgccctcgc ggccggcgag ggcatcgccg ccgagcgcga gctgcagacg
     1381 gcgctgaagc tcgcgccgga ctgggcgccg gcctggcaga tgcgcgcgcg cctggtgctc
     1441 gatcgcggcg acctgccggc tgcgctcgaa gtgctgtccg cggccgtcac gcagctgccg
     1501 aaggacacgg cactgcgcac cgcctacgcg cgcgcgctgt tcctcgccaa ccgctacgac
     1561 gaggccggcg agcagttcga ccgcctgctc gccgacaacc cgcgcgacag caacgcccgg
     1621 ctggcgctcg ccggcatcgc tttcgaggcg aagcagtacg accgcgccgt ggcgctgctg
     1681 aaggacctgc tcggcagcgg cgaaaacccc ggcgacatcg aattccagct cggtcgcgtc
     1741 gaggaggccc gcggcgatta cgcggccgcg cgcgcgtggt acgagaaggt caccgacggc
     1801 gaccgccggc tcggcgcgcg catccggctc ggcgcgctga agctcgccga aggcgacgag
     1861 cccggcatgc acgcgtggtt caatgccgag cgtgccgcgc accccgacga tgccgtcgcg
     1921 ctgttcatcg gcgaggccga ggtgctgcgc cagaaggatc tcatccagcc ggcctgggcg
     1981 gtgctgaccg ccgcgctcgc cggtcatccg gacgacaccg acctgctcta cgcccgggcg
     2041 ctggtcgccg agcggctcga tcgcatcggc gagaccgaat ccgacctgcg ccgcgtgctc
     2101 gaactcgacc ccgagcacgg gcaggcgctg aacgcgctcg gctacacgct ggccgatcgc
     2161 accgaccgtc tcgccgaggc cgaggatctc atccgccgcg cgctcgaaaa gctcccgagc
     2221 gatgccgccg tgctcgacag catgggctgg gtgctgtacc gccagggcaa ggccgccgag
     2281 ggcctgccct tcctcgaacg tgcgcagggc ctgtcgctgg agccggagat tctcgcgcat
     2341 ctggtcgagg tgctctggac gctgggccgg caggacgagg cgcgcgcgct gtgggaacgc
     2401 ggcgcgcagc gcgcgcccgg tgaccgcttc ctgcagcagg tgcgcgagaa gctgaaatgg
     2461 tgaggcgctc gcgcctgtgg cccgccgcgc ccgccgccct gctcgcactc gcgctggccg
     2521 gctgcgccga actgccggcg agccgcggcg atgccacgca ggacccgcgc tggcaggcgc
     2581 gcagcgcgca gctcgcaaag ctgcgtgact ggcagctcga tgcgcgcatc ggcgtcaacc
     2641 gcgaccggca ggcctggtcg gcgagcctga agtggcagca gcacggcccg cgctacgaga
     2701 tcgacctgct cggccccttc ggcgccgggc gcgtgcgcgt cgagggcgat gcggccggcg
     2761 tcgtgctgct gcgcggggac gacgagccgc cggtcagcgc gcgtgatccc gatgagctgt
     2821 tcgagcgcgc caccggcatc gccgtgccgg ccggggcgct cggcgactgg ctgttcggcc
     2881 ggccgcagcc cggcgtggcc gcacaggtcg aagccgacgc cgagggccgc acgacgcgca
     2941 tcgtgcaggc cggctggacc atcgagtatc cggcctggca gccggtcggc gcgctggagc
     3001 tgccggcgaa gctgcgcgcg gtgcgcggcg acgtgcaggt gcggctcgcg gtcgagcgct
     3061 gggggctgtg agccgtaccg cgatgtccga cgccggttct tctccttcct ctccttcttc
     3121 ttccccggcg ttctccgccg gctggccggc cccggccaag ctcaaccgct tcctgcacat
     3181 cgtcggccgg cgggccgatg gctatcacct gctgcagacc gtgttccagt tcatcgaccg
     3241 ggccgacacg ctggccttcg cgctgcgcga cgacggccgc atcgagcgcg aaaccgatct
     3301 gcccggcgtg ccggccgagg cggatctcgt cgtgcgcgcc gcgcggcggc tgcaggccga
     3361 gaccggcacg cgcagcggcg tcagcatccg catcgacaag cagttgccga tgggcggcgg
     3421 cctcggcggc ggttcgtccg atgccgcgac cgtgctggtg gcgctcaatc atctgtgggg
     3481 ctgcgggctc gatgacgatg cgctggccgg cctcgggctc acgctcggcg ccgacgtgcc
     3541 ggtgttcgtg cgcggccggg cggcctgggc cgaaggcatc ggcgaacggc tcacgccggt
     3601 cgagccggac gagccctggt ttctggtgct ggtgccggcg gtgcatgttg cgactgccgc
     3661 aatcttcaag gcgcctgaat tgacacggga ttcccccctc atcacaatgc gcgcctttct
     3721 cgggggggcg gggcacaacg actgcgaggc ggtcgtccat gcccgtcatc ccgaggtcgc
     3781 ggcagcgacg gcatggcttt cgcagcacac cacggcgcgg ctgaccggca ccggtgcctg
     3841 cgtgttcggc gcgttcgcgg acgaagctgc ggcccgcacg gtcatggccg ggcgaccggc
     3901 agggctcgaa gccttcgtcg cgcgcggacg caaccgttcg ccgctgctcg accggctggc
     3961 ccgtgcgcgg gccggtgact gacaagccga tgcagcagcc gatacacaag atgcagcacc
     4021 agatgcagca caagatcatt gggccgtcgc caagcggtaa ggcaccggat tttgattccg
     4081 gcattccctg gttcgaatcc aggcggccca gccagtttcc ccccctgttc cgacggcagg
     4141 ttgacccgtg tccgacgccc gtatgatggt ctttaccggc cgcgcccacc cggagcttgc
     4201 ccagcgcatc gtcgatcacc tcggcctgcc gatgggcaag gcggtgaccg gcagcttcag
     4261 tgacggcgag tgcatggtcg agctgatgga aaacgtccgc ggtcgcgacg tgttcatcgt
     4321 gcagccgacc tcggccccga ccaatgacaa cctgatggaa ctgctggtca tggtcgatgc
     4381 gctgcgccgc tcctcggcga tccgcatcac ggccgtgatc ccgtacttcg gctactcgcg
     4441 tcaggatcgc cggccgcgct cggcgcgcgt gccgatctcg gcgaaggtcg tcgccaacat
     4501 gatcacgacg gtcggcgtcg accgcgtgct gaccatcgac ctgcacgccg accagatcca
     4561 gggcttcttc gacctgccgg tcgacaacat ctacgcgacg ccgatcatgc tggaggacct
     4621 cttccagctg cacctgccga acctggtcgt cgtctcgccc gatgtcggcg gcgtcgtgcg
     4681 cgcccgcgcg ctcgccaagc gcctgcacga tgccgatctc gcgatcatcg acaagcgccg
     4741 gccgaaggcc aacgtctcgc aggtcatgca catcatcggc gaggtcgagg gccgcgactg
     4801 catcctgatc gacgacatgg tcgataccgc cggcacgctc tgccacgccg ccaaggcgct
     4861 gaaggacgac ggtgccgcct cggtgcgcgc ctactgcacg cacgccgtgc tctcgggcaa
     4921 ggcgatcgac aacctgtcga actcggcgct cgatgagctc gtcgtcaccg acaccattcc
     4981 gctgcgcccc gaagcgaagg cctgcggccg catccggcag ctgagcgtcg ccggcctgct
     5041 cgccgagacc atccaccgca tccatctgga ggaatccgtc agctcgctgt tcggcgagtg
     5101 atccgcgcac ggacttcctg cccgagattt caaggttttt tcgccccggg gcgtctggtc
     5161 gcggacgccc cggtcgtttc atcaccgtca accggagtac gccatgagcg tcgttgcttt
     5221 tgaactgaat gccgaaaccc gcagtgagca gggcaagggt gcgagccgcc gcctgcgcat
     5281 cgcgggccgg gtgcccgccg tgctctacgg tgccggcaag gccccggaac tgctgtccgt
     5341 ctcgcagcgt gacatcctgc tgcagctcga aaacgaagcc ttctactcgc acgtgctcga
     5401 tctgaagatc ggcgatcgtg ccgagcgcgt cgtgctgcgt gacctgcagc gccacccgta
     5461 caagccgatg gtcctgcacc tcgacctgca gcgcgtcgat gcgacgcagc agatcaccgt
     5521 gcacgtgccg ctgcacttcg tgaatcagga agccgcgatc ggcgtcaagc agcagggcgg
     5581 tgtcgtcagc caccacctga gcgaaatcga ggtcacctgc ctgcctgacc agattccgga
     5641 gttcatcgag gtcgacctga tcgcgctcga actcggtcag gcgctgcacg tgtcgcagct
     5701 cgtgctgccg gccggctgca cggcctacgc gctgacccac ggccagcaca gcgacgagat
     5761 cgtcgtcagc atccacgccg cccgtgccgg tggcgacgag gaagctgcgc ctgccgcata
     5821 agcgggcggc gcgcgtgcgg tcatggcgga agcgctgaaa ctcatcgccg gcctcggcaa
     5881 ccccgggccg gcctacgagc gcacgcggca caacgcgggc ttctggttcg tcgacgaact
     5941 cgcccgcagc cacggcggca gcttccgcca cgacccgaag ctcaacgccg acacctgcca
     6001 gatcacggtc gaaggccgtg cgctctggct gatgaagccg atgggcttca tgaaccgctc
     6061 cggccagccg ctcgcccggc tggccaattt ctacaagatc gcgcggccct cgatcctcgt
     6121 cgtgcacgac gagctcgacc tgccgcccgg cgtcgcgcgc ctcaagcgcg gcggcggcca
     6181 cggcggccac aacggcctgc gcgacatcat cgcccagtgc ggcggcaacg atttcctgcg
     6241 cctgcgcctc ggcatcggcc atccgggcac ggcggccgaa gtcgtcgact acgtgctgcg
     6301 caaggcgccg tcgtccgaac agcagctgat cgagcgcgcc ctcgacgacg ccctgcgcgt
     6361 gctgccgctg gtcgtgcgcg acgaattcga acgggccatg cagacgctgc acacgcgcgc
     6421 gccggcctga gccttccgcc ccatccccgt atcccgagga caccctcccc atgggcttca
     6481 agtgcggcat cgtcggcctg cccaacgtcg gcaaatccac cctgttcaac gcgctgacga
     6541 aagccggcat ccaggccgag aactacccgt tctgcaccat cgatccgaac gtcggcgtgg
     6601 tgccgatgcc ggatgcgcgc ctcgatgcgc tcgcgaagat cgtcgttccg cagcgcatcc
     6661 tgccgacgac gatggagttc gtggacatcg ccgggctggt ggccggtgcc tcgaagggcg
     6721 agggcctcgg caaccagttc ctcgccaaca tccgcgagac cgacgccatc gcccacgtcg
     6781 tgcgctgctt cgagaacgat gacgtgatcc acgtcgccgg caaggtcgat ccgctgtccg
     6841 acatcgaggt catcaacacc gaactcgcgc ttgccgacct cgccagcgtc gagaaagcct
     6901 tccagcgcgc cgagaaggcc gccaaggccg gcggcaagga cgccgtcgcc cgcaaggaac
     6961 tgctcgcccg cgtgcaggcg cacctgaacg aagccaaacc cgtgcgggcg atgaatcttt
     7021 ccgacgatga actggcgctg ctctatgagc tgcacctgct caccgtcaag ccgacgatgt
     7081 atatcgccaa cgtccacgag gacggcttcg accacaaccc gctgctcgac aaggtgcgcg
     7141 cgctcgctgc cgccgaaggc gccgaagtcg tgccggtgtg cgcggcgatc gaggccgaga
     7201 ttgcgcagct cgacgatgcc gacaagctcg aattcctcgc cgacctcggc ctcgacgaac
     7261 ccggcctgaa ccgcgtcatc cgcgccggtt acaagctgct cgggctgcag acctacttca
     7321 ccgccggcgt caaggaagtg cgcgcctgga cggtgccgat cggcgccacc gcgccgcagg
     7381 ccgccggcgt catccacacc gacttcgaac gcggcttcat ccgcgccgaa gtcatcgcct
     7441 acgacgactt catcgccggc aacggcgagg atggcgccaa gaaggccggc aagtggcgcc
     7501 tcgaaggcaa ggactacgtc gtgcaggacg gcgacgtgat gcacttccgc ttcaacgtct
     7561 gatcgcggct gccccggctg cccgcattgt cccgcccctg cgccggccgc tacgctcgcg
     7621 gcacgaggga aggggagggt gcgcgatgcc gggcaaggtg tttgtcagtt acagc
//
LOCUS       DAOQIE010000193         7592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5052_length_7592_cov_7.528592, whole genome shotgun sequence.
ACCESSION   DAOQIE010000193 DAOQIE010000000
VERSION     DAOQIE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5052_length_7592_cov_7.528592"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK144_12195"
     CDS             <1..768
                     /locus_tag="PK144_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNF66533.1"
                     /translation="ITLNCNGIRSAAGKGFFEWLAGQQADIVCLQETKSQEHQRGREF
                     RPQGWHCHYVDAVRPGYSGVAILSKREPDAVVTALGAGFEDMDAEGRYLEARFGALSV
                     VSLYLPSGSSGELRQQVKYSFMDRFAGHLAGLRARGGEALVCGDWNIAHRPADLRNWK
                     SNQKNSGFLPEERAWLDRLYGEAGWIDVWRRLYPEDGEACYTWWSNRGQAWAKNVGWR
                     LDYHVACPGFAGRAQAGSVYKAARFSDHAPVTIDYAD"
     gene            842..4111
                     /locus_tag="PK144_12200"
     CDS             842..4111
                     /locus_tag="PK144_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66534.1"
                     /translation="MPSFPLRLRTRFRLLLAASVLGIALVDALVAWSNLDERVYTELL
                     HEARDVRDVLLGTREVYRALLPDGDSAPLDAHMLALLPSHALLRIARDFPAPDDAEAS
                     LRLAVVSDRPLDPANRADAGQQQALDWLRAHPQAGEHMREVRDSAGERHLLYALPIRF
                     DGWCLRCHAPTASAGETGIHGLLSLRSPLAPAREREFAEWSHNFAGRMLAYIALFGLL
                     GLLVDRGFVRRLAQLERAALAVADGDASVRVQSDGPRPAQDELATLGQAFDAMAAAVQ
                     ARTGELQTALAQYRYLFAHSPLPMLVFDRTTLAILDVNEAALAHYGYAREAFLQLTIS
                     ELRPPEDVALMQQLRAAIAAAGEIPACHTVRHRRSDGTLLDVNVWTRALDFDGRPARI
                     ALIQDVSEQQRALAALRLSQLKYQTIFRTSPDLIAITERASGRFLEINDAYTRIMGYT
                     AEEAIGRTSAELGTWGAPDSRAQMIAALGEGVRLMNFQTLFRRKDGEVFPALLSLEAA
                     RIGEHDCFIISARDITERIRVEQALADSEARFHTMIDWTHNWEYWLGSDRSLRCTSPS
                     VLRISGYTATELHADPGLIDALVHPEDRERWQMHLAEEAGSDAVLELQLRLLTREGQT
                     RWVAHTCRPVFDGAGLDCGRRVTVRDITDEKAAADEIRELAFYDTLTHLPNRRLLLDR
                     AGRALAASTRSGEYGALLMLDLDHFKKLNDTQGHDVGDRLLIEVARRLRGCIRQHETV
                     ARLGGDEFVVLLETLGTGEAEALAHAGRVSAKIRSVLAAPYALGPGPALFHSTPSLGV
                     TLFCGAGAAVETLLKQADVALYQAKDAGRHTVRFFAPAMQAAIDRRMQLEAALRGGLE
                     RDEFRLWYQPQVDAAGRRIGAEALLRWCPVDGPMVAPAEFIGLAEETGLIVPIGQWVL
                     DQACAQLREWQAEPATRALRLAVNVSARQFHEPDFVERVQRSVQTSGIDATGLKIELT
                     ESVVLDQIDEVIGRMHALHALGVGFSLDDFGTGYSSLSYLRRLPLDQVKIDQSFVRGI
                     ARHGEDAAIVAAIVAMCRTLNFAVIAEGVETVEQRDALLACGCEAFQGYLYGRPAPAG
                     H"
     gene            complement(4132..5559)
                     /locus_tag="PK144_12205"
     CDS             complement(4132..5559)
                     /locus_tag="PK144_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66535.1"
                     /translation="MALPSMTFPERPLQVSPPGSGRIANLGTLLWTTLLLLASAYFLL
                     PPIADLRDDFTIADTARPVQGGRVEGRCRTSLGVLVRCTATLTAPGRGGPQLQREVDY
                     FFVDFHLGDYTATVVADPVRPALLSTDLALERLWNRVYTLATAIPLGLGLLFGALRLA
                     GQRIGERRSAVDALSGHVLHPVLLRLDSYLPGRWTVTPLPPTRDRRSRTWPVPRAARP
                     IVMDPERRLVLGVAAGSEELAMPLDTGLSWIGLERGERLRLREQLGPDRLGGWLPNLG
                     RAGLDDERAALSRRARRLGLSGGGLALLAALAAWVAWGGHDFSGDAIVLRRGDEGPAS
                     GEVRLNGIRHTELAVQTTRVDARAAHLEVWVPLTAPDWQAGQPVVWLIDDPEYGRGEP
                     RAATLVGSVAGPVPDRAREQLARMHVQLSPEARVLAARAWEPPWQLPAIIVSGIGGTL
                     GAGLLLGAALLGLRVRALSAQLRSR"
     gene            complement(5726..7126)
                     /locus_tag="PK144_12210"
     CDS             complement(5726..7126)
                     /locus_tag="PK144_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNF66536.1"
                     /translation="MTARRPRRVLLAMIALAAGLAAAPVPAALPWGAGSSELPTLAPM
                     LEKATPAVVNVNTESHVRTPRNPLLDDPVFRRFFNLPKQPHERERKAQSLGSGVIVDA
                     ERGYVLTNHHVIEGADVISITLRDGRQLQAKLIGSDPDTDVAVVQVPAKNLSALPLAD
                     SDGLRVGDFVVAIGNPFGLGQTVTSGIVSALGRSGLGIEGYEDFIQTDASINPGNSGG
                     ALVNLRGELIGINTAILAPSGGNVGIGFAIPINMARQVMDQIVRHGAIRRGLLGVATQ
                     DLTPQLAEAFGIRTDQGAVISQIGAKSAAEKAGLRVGDVITAVNDRPVRDSAALRNSI
                     GLLRVGDEVRLGLLREGKPLSLTARLGEGRGGRVADSSGGGGKALNPRLEGASFGNLP
                     DGHPLSGEVDGVLVNAVEAGSPAARGGLRRGDVILGVNREPVAGIEDFAQAVTGNRAL
                     LLQVLRGRSSLFLMLQ"
     gene            complement(7186..>7592)
                     /locus_tag="PK144_12215"
     CDS             complement(7186..>7592)
                     /locus_tag="PK144_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNF66537.1"
                     /translation="IEAGRKIRLAGQGEAGAHGGADGDIYLEIAYAPHPRYRAEGRDL
                     TLELPVAPWEAALGARVEVPTLAGPVTLAIPAGAQSGQKLRLRGRGLPGEPAGDQYVR
                     LKIVNPPADSEAARALFRRLQDELPFDPRAGL"
BASE COUNT         1067 a         2674 c         2784 g         1067 t
ORIGIN      
        1 atcacgctga actgcaacgg catccgttcc gcggccggca agggcttttt cgaatggctc
       61 gccgggcagc aggccgacat cgtctgcctg caggaaacca agtctcagga acaccagcgt
      121 ggccgtgaat tccggccgca gggctggcac tgccattacg tcgatgccgt gcgccccggc
      181 tacagcgggg tggcgatcct gtcgaagcgc gagcccgatg ccgtcgtcac cgcgctcggt
      241 gccggcttcg aggacatgga cgccgaaggc cgttatctcg aagcccgctt cggggcgctg
      301 agcgtcgtgt cgctgtacct gccctcgggg tcgagcggcg agctgcgcca gcaggtcaag
      361 tattcgttca tggaccgctt cgccgggcat ctggcgggct tgcgcgcgcg cggcggcgag
      421 gcgctggtct gcggcgactg gaacatcgcg caccggccgg cggatctcag aaactggaaa
      481 tccaaccaga agaacagcgg cttcctgccc gaagagcgtg cctggctgga ccggctgtac
      541 ggcgaggccg gctggatcga cgtctggcgc cggctgtacc cggaggacgg cgaggcctgc
      601 tacacgtggt ggtcgaaccg cggccaggcc tgggcgaaga acgtcggctg gcgtctcgac
      661 taccacgtcg cctgcccggg cttcgccggc cgggcgcagg ccggcagcgt ctacaaggcc
      721 gcgcgctttt ccgaccatgc gccggtaacg atcgattacg cggattgatt cggcttacaa
      781 aaccatcatg atgatcaggt tttccgatcc ctgagccgct gccgatcccg attccacgat
      841 ggtgccgagt ttccccctgc ggctgcgtac ccggttccgg ctgctgctgg cggcctcggt
      901 gctcggcatc gcgctggtcg atgcgctggt tgcctggtcc aacctcgatg aacgcgtcta
      961 cacggagctg ctgcacgagg cgcgcgacgt gcgcgacgtg ctgctcggca cgcgggaggt
     1021 ctaccgcgcg ctcctgcccg acggagattc cgcgccgctc gatgcgcaca tgctggcgct
     1081 gctgccctcg catgcgctgc tgcgcatcgc ccgcgacttc ccggcccccg acgatgcgga
     1141 ggcttcgctg cggctggcgg tcgtctcgga ccggcccctc gatccggcca accgcgccga
     1201 tgccgggcag cagcaggcgc tggactggct gcgggctcat ccgcaggccg gcgagcacat
     1261 gcgcgaggta cgcgacagcg ccggcgagcg gcatctgctg tacgcgctgc cgatccgctt
     1321 cgatggctgg tgcctgcgct gccatgctcc cacggcatcg gccggcgaga ccggcatcca
     1381 cggcctgctg agcctgcgct cgccgctggc cccggcccgc gagcgggaat tcgcggaatg
     1441 gagccacaac ttcgcgggcc gcatgctcgc ctacatcgct ctgttcgggc tgctcggcct
     1501 gctggtcgat cgcggcttcg tgcgccggct ggcgcagctg gaacgggcgg cactggcggt
     1561 ggccgatggc gatgcctcgg tgcgcgtgca gagcgacggg ccccggccgg cgcaggacga
     1621 gctggccacg ctcggtcagg ccttcgatgc gatggcggcg gcggtgcagg cgcgcaccgg
     1681 cgaactgcag acggcgctcg cgcagtaccg ctacctgttc gcgcacagtc cgctgccgat
     1741 gctggtcttc gaccgcacga cgctcgcgat cctcgatgtc aacgaggccg ccctcgccca
     1801 ctacggctat gcgcgcgagg cgttcctgca gctgacgatc agtgagctgc gcccgcccga
     1861 ggacgtggcg ctgatgcagc agctgcgcgc cgcgatcgcc gctgccggcg agattccggc
     1921 ctgtcacacc gtgcgccacc gccgcagcga cggcaccctc ctcgatgtca acgtctggac
     1981 acgggcgctg gatttcgatg gccggccggc gcgcatcgcg ctgatccagg atgtcagcga
     2041 gcagcagcgc gcgctggcgg cgctcaggct ctcgcagctg aagtaccaga ccatcttccg
     2101 cacctcgccg gacctgatcg cgatcaccga gcgcgccagc gggcgcttcc tcgaaatcaa
     2161 cgatgcctat acgcgcatca tggggtacac ggccgaggag gccatcggcc gcacctcggc
     2221 cgaactcggc acctggggcg cgccggacag ccgtgcgcag atgatcgccg cgctcggcga
     2281 gggcgtgcgc ctgatgaact tccagaccct gttccggcgc aaggacggcg aggtgttccc
     2341 ggcgctgctg tcgctggagg ccgcgcgcat cggcgagcac gactgcttca tcatctcggc
     2401 gcgcgacatc accgagcgca tccgcgtcga gcaggcgctg gccgacagcg aggcgcgctt
     2461 ccacacgatg atcgactgga cccacaactg ggagtactgg ctcggcagcg accgcagcct
     2521 gcgctgcacc agtccgtcgg tcctgcgcat cagcggctac acggcgaccg agctgcacgc
     2581 cgatccgggc ctgatcgatg cgctggttca ccccgaggac cgcgagcgct ggcagatgca
     2641 tctggccgag gaagccggca gcgacgcggt gctggagctg cagctgcggc tgctgacgcg
     2701 ggaggggcag acgcgctggg tcgcccacac ctgccggccg gtgttcgatg gcgcggggct
     2761 cgactgcggg cggcgcgtga cggtgcgcga catcaccgac gagaaggctg cggccgacga
     2821 gattcgcgag ctggccttct acgacacgct gacgcacctg ccgaaccgcc ggctgctgct
     2881 cgatcgcgcc ggccgggcgc tggcggcgag cacgcgcagc ggcgagtacg gtgcgctgct
     2941 gatgctcgat ctcgatcact tcaagaagct caacgacacc cagggccacg atgtcggcga
     3001 ccggctgctg atcgaggtcg cgcggcggct gcgcggctgc atccgccagc acgagacggt
     3061 cgcgcgcctc gggggcgacg agttcgtggt gctgctggag accctcggca ccggcgaggc
     3121 cgaagccctc gcgcacgccg ggcgcgtcag cgccaagatc cgcagcgtgc tggccgcgcc
     3181 gtatgcgctc gggccggggc cggcgctgtt ccattcgacg ccgagcctcg gcgtgacgct
     3241 gttctgcggg gccggcgcgg cggtcgagac gctgctgaag caggccgacg tggcgctgta
     3301 ccaggccaag gatgccggcc ggcacacggt gcgcttcttc gcgccggcga tgcaggcggc
     3361 gatcgaccgg cgcatgcagc tcgaagcggc gctgcgcggc gggctcgaac gcgacgagtt
     3421 ccggctctgg tatcagccgc aggtcgatgc cgcaggccgg cgcatcggcg ccgaggcctt
     3481 gctgcgctgg tgtccggtcg acgggccgat ggtcgcgccg gccgagttca tcgggctggc
     3541 cgaggaaacc gggctgatcg tgccgatcgg ccagtgggtg ctggaccagg cctgcgcgca
     3601 gctgcgcgaa tggcaggcgg agccggcgac gcgcgcactg cgtctggcgg tcaacgtcag
     3661 cgcccgccag ttccacgagc cggacttcgt cgagcgcgtg cagcgcagcg tgcagacgag
     3721 cggcatcgat gccacgggcc tgaagatcga gctgaccgag agcgtcgtgc tcgatcagat
     3781 cgacgaggtg atcgggcgca tgcacgcgct gcacgcgctc ggcgtcggct tctcgctcga
     3841 tgacttcggc accggctact cgtcgctgtc ctacctgcgc cggctgccgc tcgatcaggt
     3901 caagatcgac cagagcttcg tgcgcggcat cgcccggcac ggcgaggatg cggcgatcgt
     3961 cgcggcgatc gtcgcgatgt gccggacgct gaacttcgcg gtgatcgccg agggcgtcga
     4021 gaccgtcgag cagcgcgatg cgctgctcgc ctgcggctgc gaggccttcc agggctatct
     4081 gtacggccgg ccggcaccgg ccggtcactg aggcgcgccg ccgccgccgc gtcagcggct
     4141 gcgcagctgt gccgacagcg cacgcacgcg caggccgagc agggccgcac cgagcagcag
     4201 gccggcgccg agcgtgccgc cgatgccgga gacgatgatc gccggcagct gccacggcgg
     4261 ctcccaggcg cgggcggcga gcacgcgcgc ctccgggctc agctgcacgt gcattcgcgc
     4321 cagctgctcg cgcgcgcgat ccggcaccgg cccggcgacg ctgccgacga gggtcgcggc
     4381 ccgcggctcg ccgcggccgt attcgggatc gtcgatcagc cagaccaccg gctgaccggc
     4441 ctgccagtcc ggggcggtca gcggcaccca gacctccaga tgcgcggcgc gcgcatcgac
     4501 gcgcgtggtc tgcacggcga gttcggtgtg gcggatgccg ttcaggcgca cctcgccgct
     4561 ggccgggcct tcgtcgccgc gcctcagcac gatggcatcg ccgctgaaat catgaccgcc
     4621 ccaggccacc caggccgcca gcgcagcgag cagcgccagc ccgcccccgc tcagcccgag
     4681 ccgccgggcc cggcgcgaca gggccgcacg ctcgtcatcg aggccggcgc gcccgaggtt
     4741 cggcagccag ccgccgaggc gatcggggcc gagctgttcg cgcaggcgca ggcgttcgcc
     4801 gcgctccagc ccgatccacg acaggccggt gtcgagcggc atcgccagtt cctcgctgcc
     4861 ggcagcgaca ccgagcacca gccgccgctc cgggtccatg acgatcggac gcgctgcccg
     4921 cggcaccggc caggtgcgcg agcggcgatc gcgggtcggc ggcagcggcg tcacggtcca
     4981 gcgcccgggc aggtagctgt ccagacgcag cagcaccggg tgcagcacgt ggccggacag
     5041 cgcatcgacg gcgctgcgcc gctcgccgat gcgctgtccg gccaggcgca gcgcgccgaa
     5101 cagcaggccg aggccgagcg ggatggcggt cgcgagcgtg tagacgcggt tccacagccg
     5161 ttcgagcgcc agatcggtgc tgagcagcgc cgggcgcacc ggatcggcga ccacggtcgc
     5221 cgtgtagtcg ccgaggtgga aatcgacgaa gaagtagtcg acctcgcgct gcagttgcgg
     5281 cccgccgcgt ccgggtgccg tcagcgtcgc cgtgcagcgc accagcacgc cgagcgaggt
     5341 ccggcagcgg ccctcgaccc tgccgccctg caccgggcgg gcggtgtcgg cgatcgtgaa
     5401 gtcgtcgcgc aggtcagcga tcggcggcag caggaaatac gcgctggcga gcagcagcag
     5461 cgtggtccac agcagcgtgc cgaggttggc gatgcggccg ctgccgggcg ggctgacctg
     5521 gagcggacgt tcggggaacg tcatggatgg cagggccatg gacggcgcct cctcgtattg
     5581 gggaaagccg tccagggcgt aacgggtgcg acattcagtc gctacggtgc tgagggcagg
     5641 aagataccgc gggccggcgg cgacacttgt tgcagaacgt cacacggccg gcccgggcaa
     5701 gcgcgggtgg ccgggctttg cgcagctatt gcagcatcag gaacagcgag ctgcgcccgc
     5761 gcagcacctg cagcagcagc gcgcggttgc cggtgaccgc ctgcgcgaag tcctcgatgc
     5821 cggcgaccgg ctcgcggttg acgccgagga tcacgtcgcc gcggcgcagg ccgccgcgcg
     5881 cggccggcga accggcctcg acggcgttga ccagcacgcc gtcgacctcg ccggacagcg
     5941 gatggccatc ggggaggttg ccgaagctcg cgccttcgag gcgcggattc agcgccttgc
     6001 cgcctccgcc actgctgtcg gcgacgcgcc cgccgcgacc ttcgccgagc ctggcggtga
     6061 ggctgagcgg cttgccctcg cgcagcaggc cgaggcgcac ctcgtcgccg acgcgcagca
     6121 ggccgatgct gttgcgcagc gccgccgaat cgcgcaccgg ccggtcgttg acggcggtga
     6181 tcacgtcgcc gacgcgcagg ccggccttct cggcggcgct cttcgcgccg atctgcgaga
     6241 tcaccgcgcc ctgatcggtg cggatgccga aggcctcggc gagctgcggc gtcaggtcct
     6301 gcgtggcgac gccgagcagg ccgcggcgga tcgcgccgtg gcgcacgatc tggtccatga
     6361 cctggcgtgc catgttgatc gggatcgcga agccgatgcc gacgttgccg cccgaaggcg
     6421 cgaggatcgc ggtgttgatg ccgatcagct cgccgcgcag gttcacgagc gcgccgccgg
     6481 agttgccggg gttgatcgag gcgtcggtct ggatgaagtc ctcgtagccc tcgatgccga
     6541 gccccgagcg cccgagcgcc gagacgatgc ccgaggtcac ggtctggccg agcccgaagg
     6601 gattgccgat tgcgacgacg aaatcgccga cgcgcaggcc gtcggaatcg gcgagcggca
     6661 gggcgctgag gttcttcgcc ggcacctgca cgacggcgac atcggtgtcc gggtccgagc
     6721 cgatcagctt cgcctgcagc tggcggccgt cgcgcagcgt gatgctgatc acgtcggcgc
     6781 cttcgatgac gtgatggttg gtcagcacgt agccgcgctc ggcatcgacg atgacgccgg
     6841 agccgagcga ctgcgccttg cgttcgcgct cgtgcggctg tttcggcagg ttgaagaagc
     6901 gacggaacac cgggtcatcg agcagcggat tgcgcggcgt gcgcacgtgg ctctcggtgt
     6961 tgacgttgac gacggccggc gtggccttct ccagcatcgg cgcgagcgtc ggcagctcgc
     7021 tgctgccggc gccccagggc agcgccgccg gcacgggcgc agcggcgagg ccggcggcga
     7081 gtgcgatcat cgcgagcagg acacggcggg ggcggcgggc agtcatcggg cagctccggg
     7141 tcatcggcag tcggtcgtca aaggctggaa ggctgcacgc cggactcaga gaccggcgcg
     7201 cgggtcgaac ggcagttcat cctgcagccg gcggaacagc gcgcgtgccg cctcgctgtc
     7261 ggccggcgga ttgacgatct tcaggcgcac gtactggtca ccggccggct cgccgggcag
     7321 gccgcggccg cgcaggcgca gcttctggcc gctctgcgca ccggccggga tcgccagcgt
     7381 caccggcccg gccagcgtcg gcacctcgac gcgcgccccg agcgcggcct cccacggcgc
     7441 gaccggcagc tccagcgtca ggtcgcggcc ttcggcgcgg tagcgcgggt gcggcgcgta
     7501 ggcgatctcc agatagatgt cgccatcggc gccgccgtgc gccccggcct cgccctggcc
     7561 ggccagacgg atcttcctgc cggcctcgat gc
//
LOCUS       DAOQIE010000194         7590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5055_length_7590_cov_7.843663, whole genome shotgun sequence.
ACCESSION   DAOQIE010000194 DAOQIE010000000
VERSION     DAOQIE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5055_length_7590_cov_7.843663"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..289)
                     /locus_tag="PK144_12220"
     CDS             complement(2..289)
                     /locus_tag="PK144_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66538.1"
                     /translation="MVGPQLPGQDGLPPSLFRTEARTVQVQPQACPHLSWSTMLTELR
                     AARLRGASASERLMMLDRWRRERCENCTITCLYPMPELPSALREQYEWRPR"
     gene            586..1464
                     /locus_tag="PK144_12225"
     CDS             586..1464
                     /locus_tag="PK144_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF66539.1"
                     /translation="MSFPSTAPGTRAPLIGVALTLLAVLCFAVLDSSAKYLSQHYPVA
                     MVVWARYAVHTLLMLIAYAPARGRTLLHTRRPGRQLLRALLLLSVTILFVSGLRFLPL
                     AEATAIIYLTPLLVTALSVPLLGERVARGTWIAILTGLAGVLIVVRPGGALLGWTVLL
                     PLAAALCNSLYQILTRSFAGSENGTTTNFITGLTGTVLASLTLPWVWTTPTVAHAGLM
                     IVLGCAGFGGHALLIRAFEHASPAVLGPLTYGQLAIATLLGFGVFGTLPDAASFAGMA
                     LIVAGGLLVLLPRRAG"
     gene            complement(1474..1971)
                     /locus_tag="PK144_12230"
     CDS             complement(1474..1971)
                     /locus_tag="PK144_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66540.1"
                     /translation="MPLQTDPASAAAEELLQQAEASRRAGDPALAAALYQRVLNLWPE
                     SPAARIAAGRLDTLGLPDPAPAEALASAGDLPGDDSYLPGELAAACLRAAALVCLVVL
                     SLMAGWLLVDAAVGHGPSDSRAWLPGVLVLLAAIVLPALLATIAGIGLNLVALRRRQE
                     TLRLR"
     gene            2123..2662
                     /locus_tag="PK144_12235"
     CDS             2123..2662
                     /locus_tag="PK144_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNF66541.1"
                     /translation="MTSVVCSVTVSLDGFLIPPGSAREEIDGGRTPLPEPGDRLPAPE
                     VIIVGRHTAAAARRAAHWPWPQQTVIALLHSESPAERDARLCYSTETPSGLLARLQRD
                     GITRAQLAGGSTLISVFHAAGLIDEWLLRVAPVLLGGGRPLFGPGTHPSTLALVHAWG
                     YPDGAVRLHYRRGDAGIPA"
     gene            2920..6087
                     /locus_tag="PK144_12240"
     CDS             2920..6087
                     /locus_tag="PK144_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66542.1"
                     /translation="MSSEAVRERCARAVRRWSVLVPFALPVVLLVLLAGREAAPLLLP
                     LLVLAARIELRAAGALAACGLALVLLADRLGPLLLIDAAWFAPLVQVLALIACLGLAV
                     YPSVPVIRRAGGDDEAGRERTRLGALLSSLDDLAFEFDADGRYLDVWTARPELLLMPR
                     EQLLQRNLHDVLGQAEGDRVLGILRAVLADGEPREVEVRIRLADGRERQMLGRCARIA
                     PSVATAAATVSCLVRDVTAQRQVEQALRDSEARLRTILETLPVGAVHHHVPSGTVTLN
                     HAAERLLHYARAEVQRVEAWFARLYGEQAETVLALYLADRERGLDQVRTLDVHCGDGL
                     TRRVQFAGCASGEHEVWVMTDVTAHERLQQRLQATQAAVDAAEDALYVMGEDGRFVDV
                     NPAACRRLGWSREELLTMGVTDIDPLVGLEQLRAMREEVARKGHVRVESEHCTRDGER
                     FPVEIVATGMPYGDGQYNCAIVRDISARRAAETQLRLYAEVFEHAAESIMITDAGCSI
                     VAVNPAFTTVTGYAPDEVLGRNPRMLASGRQDAAFYRAFWQSLDAHGLWQGEIWNRRK
                     SGEVFPEWLSVSVVRDADAGPQHYIAVFTDLSEHKAHERRIQFLAQYDPLTRLPNRVL
                     LFERLEHALAAARRGARRCALLFLDLDRFKNVNDSLGHGIGDRLLEAVAQRLRAALRD
                     CDTVARLGGDEFVVLLEDLPAGDGEREAALVAGKLLAALAQPFQIDTHELRITSSVGI
                     SLYPGHGADAETLIRSADTAMYHAKQSGRDNFQFFTEDMNARVLERLTLENGLRRALA
                     QQGFALVWQPQLAIGDGRLIGFEALLRWPQPEGRAQVPPAQFIPVAEDCGLIGAIGDW
                     VLDAACRQLCAWRAAGLPGVRIAVNLSALQLRDARLADKVAAALAVCGLDGAALELEV
                     TESALMSDPPQTAGTLARLRALGVQLAIDDFGTGYSSLAYLKQFPIDRLKIDRSFVRD
                     LASDPNDAAIARTIITLAHSLGLRVIAEGVETPAQLGFLRGHGCDEAQGYLYAPPLSP
                     EQAAEVLAAGRIRPAASTGTDAGPA"
     gene            complement(6197..6391)
                     /locus_tag="PK144_12245"
     CDS             complement(6197..6391)
                     /locus_tag="PK144_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66543.1"
                     /translation="MLRIGQWTLRLALLLIVAGLLGGFGLMLAGHADTGMTLVTLVPF
                     GFVIGFAGLALVVLHEKRDR"
     gene            complement(6455..7276)
                     /locus_tag="PK144_12250"
     CDS             complement(6455..7276)
                     /locus_tag="PK144_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HNF66544.1"
                     /translation="MSDLPATSAADPEEPAGGKPRRRTRRWYRRPMLWLLLVFVFPVV
                     VQAARHALSATDQPWYAARHDSARLVPEAGRQPEAIVQAWAARAWGWRGIFGVHTWIA
                     VKPAGAPAWQRLEVIGWRLYRGGSAVSITSGVPDAYWYGNRPLLLAELRGAAAADAIP
                     RLLDAARRYPYADRYRVWPGPNSNTFVAQLARAVPELRLALPATAIGKDYPVDGRWLA
                     PAPSGTGWQLTLGGLAGLTLAVEEGLEIDLLGLTAGIDPGGPALKLPGIGRIGPP"
BASE COUNT         1016 a         2707 c         2737 g         1130 t
ORIGIN      
        1 ctcagcgcgg gcgccactcg tactgttcgc gcagcgccga tggcagctcg ggcatcggat
       61 acaggcaggt gatcgtgcag ttttcgcagc gctcgcggcg ccagcgatcg agcatcatca
      121 ggcgttccga cgccgatgcg ccacgcaggc gcgcagcacg caactcggtg agcatcgtgc
      181 tccaggacag atgcggacag gcttgcggct gtacctgcac ggttcgagcc tcggttcgaa
      241 acagggaagg gggcaaccca tcctgccccg gaagctgcgg gccgaccatc cgggcggctc
      301 ggaatccggc ctgagaaatt tctcagctgc acggggcgat gaagattcgt ccacctgcac
      361 cccgttcgcg cagcttctcc tacgctttgc tggcaaccga tccggttgtc acccccgtct
      421 ggaaagcact ggcgacaggt tcccaccccc gcggacctgt cgccgatttt ttttgcacgt
      481 ttccggcgtg cccgatgcac ggctgcacgg ctgccgtacc ggcgcggcag ccccgcaagc
      541 cagcggctac gatgcgcagc cccgcgccgc cgtaccggat cgatgatgag cttcccgtcc
      601 accgcccccg gcacgcgagc gccgctgatc ggcgtcgccc tcacgctgct cgccgtgctg
      661 tgcttcgccg tgctcgacag cagcgccaaa tacctctcgc agcactatcc ggtggcgatg
      721 gtggtctggg cgcgctacgc ggtgcatacg ctgctgatgc tgatcgccta tgccccggcg
      781 cgcggacgca cgctgctgca cacgcgccgg cccgggcgcc agctgctgcg ggcgctgctg
      841 ctgctgtcgg tgaccatcct gttcgtcagc ggcctgcgct tcctgccgct cgccgaggcg
      901 accgcgatca tctacctgac gccgctgctg gtgacggcgc tgtcggtgcc gctgctcggc
      961 gagcgcgtcg cccgcggcac ctggatcgcg atcctgaccg ggctggccgg cgtgctgatc
     1021 gtcgtgcgcc cgggcggggc gctgctcggc tggacggtgc tgctgccgct ggcggcggcg
     1081 ctctgcaaca gcctgtacca gatcctgacg cgcagcttcg ccggcagcga gaacggcacg
     1141 accaccaact tcatcaccgg cctgaccggc accgtgctgg ccagcctgac gctgccgtgg
     1201 gtgtggacga cgccgacggt cgcgcacgcc gggctgatga tcgtgctcgg ctgcgccggc
     1261 ttcggcgggc atgcgctgct gatccgcgcc ttcgagcacg cctcgccggc cgtgctcggg
     1321 ccgctgacct acggccagct cgcgatcgcg acgctgctcg gtttcggcgt gttcggcacg
     1381 ctgccggatg cggcctcgtt tgccggcatg gcgctgatcg tcgccggcgg actgctggtg
     1441 ctgctgccgc ggcgcgcggg ctgaacgccg gcctcagcgc aggcgcagcg tttcctggcg
     1501 ccggcgcagc gccacgaggt tcaggccgat gccggcgatg gtcgcaagca gcgccggcag
     1561 cacgatcgcg gcaagcagca ccagcacgcc cggcagccag gcgcggctgt ccgacgggcc
     1621 atggccgacc gcggcatcga ccagcagcca gccggccatc agcgacagca cgaccagaca
     1681 gaccagcgcc gccgcgcgca ggcaggcagc ggccagctcg ccgggcaggt agctgtcgtc
     1741 gccgggcagg tcgccggcgc tcgcgagcgc ctcggccggc gccgggtccg gcaggccgag
     1801 cgtgtcgagg cgaccggcgg cgatgcgcgc ggccggggac tcgggccaga ggttcagcac
     1861 gcgctggtac agcgcggcgg ccagcgccgg atcaccggcg cggcgcgagg cttcggcctg
     1921 ctgcagaagt tcttcggcgg cagccgaggc gggatcggtt tggaggggca tggccgggtt
     1981 ccgtggcagg acgcgcgcga ctctgcgcag ccgccgccgc gccggcaatc ctacgaaacg
     2041 actattcggc acgcggacgg gccgtggcgc cggcgacggc tatgctcggc gatcgctatc
     2101 gtcccccgcc ggagccgtgc ccatgaccag cgtcgtctgc agcgtcaccg tcagcctcga
     2161 cggtttcctg atcccgcccg gcagcgcgcg ggaggagatc gacggcggcc ggacgccgct
     2221 gcccgagccc ggcgaccgcc tgcccgcgcc cgaagtcatc atcgtcggcc ggcacacggc
     2281 cgccgcggca cgccgggccg cgcactggcc gtggccgcag cagaccgtca tcgcgctgct
     2341 gcacagcgaa tccccggccg agcgggatgc gcggctctgc tacagcaccg aaaccccgtc
     2401 cgggctgctg gcgcgcctgc agcgcgacgg catcacgcgt gcgcagctcg ccggcggcag
     2461 cacgctgatc tcggtcttcc acgccgccgg cctgatcgac gaatggctgc tgcgcgtggc
     2521 gccggtgctg ctcgggggcg gccggccgct gttcgggccg ggcacgcatc cctcgacgct
     2581 ggcgctggtg catgcctggg gctatccgga tggcgccgtg cgcctgcact accgtcgcgg
     2641 tgacgccggc attcctgcgt gagtccacgt aaaagccggt ttcgaaatgg attcctgtct
     2701 gctgcaagcc ggtgtcgccg gttgccggcg ccacacggca agtcgatgat tatcggttaa
     2761 ataccagcaa tgccgcgccg gggacggcgc tgcctgtccg ccggtgctgc gttccgtgcc
     2821 cgcttgaggc ccggcacctg cgggtgcgag gctcgcgccc cgtgcccgcc agccgtctcc
     2881 gccggcgctg ccgtcccccc gccttccggt atgttgccga tgtcgtctga agctgtccgc
     2941 gaacgctgcg cgcgcgccgt tcgccgctgg tccgtcctcg ttccgttcgc gttgccggtg
     3001 gtgctgctgg tgctgctcgc cgggcgggag gctgcaccgc tgctgctgcc gctgctggtc
     3061 cttgctgccc gcatcgaact gcgcgccgcg ggcgcactcg ccgcctgcgg cctggcgctg
     3121 gtgctgctcg ccgaccgcct cgggccgctg ctgctgatcg acgccgcctg gttcgcgccg
     3181 ctggtgcagg tgcttgcgct gatcgcctgt ctggggctcg ccgtgtaccc gtcggtgccg
     3241 gtaatccgcc gggcgggtgg ggacgacgag gccggccggg agcgcacccg gctcggcgcg
     3301 ctgctgtcct cgctcgatga tctggccttc gaattcgatg ccgacggccg ctatctggac
     3361 gtctggaccg cccggcccga gctgctgctg atgccgcgcg agcagctgct gcagcgcaac
     3421 ctccacgacg tgctcgggca ggccgagggc gaccgggtgc ttggcatcct gcgggccgtg
     3481 ctcgccgacg gcgagccgcg cgaggtcgag gtccgcatcc ggctcgccga tggccgcgag
     3541 cggcagatgc tcgggcgctg cgcacgcatc gcgccgtccg tggccactgc ggcggcgacg
     3601 gtcagctgcc tcgtgcgcga cgtgaccgcg cagcgtcagg tcgagcaggc gctgcgcgac
     3661 agcgaggcgc ggctgcggac catcctcgaa accctgccgg tcggtgccgt ccaccatcac
     3721 gtgccgagcg gcaccgtgac gctgaaccac gccgccgaac ggctgctgca ttacgcgcgc
     3781 gccgaggtgc agcgcgtcga ggcctggttc gcgcggctgt acggcgagca ggccgaaacc
     3841 gtgctggcgc tgtatctggc cgatcgcgag cgcgggctcg atcaggtgcg cacgctcgac
     3901 gtgcactgcg gcgacggcct cacgcgccgc gtgcagttcg ccggctgcgc gagcggcgaa
     3961 cacgaggtct gggtgatgac cgacgtcacg gcacacgagc gcctgcagca gcgcctgcag
     4021 gcgacgcagg ccgccgtcga tgccgccgag gacgcgctgt acgtcatggg cgaggacggc
     4081 cgcttcgtcg acgtgaatcc ggccgcctgc cgccggctcg gctggtcgcg cgaagagctg
     4141 ctgaccatgg gcgtcaccga catcgacccg ctggtcgggc tcgaacaact gcgtgcgatg
     4201 cgcgaagagg tcgcgcgcaa gggccatgtc cgcgtcgaat ccgagcactg cacgcgcgac
     4261 ggcgaacgct ttccggtcga gatcgtcgcg accgggatgc cgtacggcga cggccagtac
     4321 aactgcgcga tcgtgcgcga catcagcgcc cggcgcgccg ccgaaaccca gctgcggctg
     4381 tacgccgagg tgttcgagca cgccgccgag tcgatcatga tcaccgacgc cggctgcagc
     4441 atcgtcgccg tgaatccggc gttcacgacg gtgaccggct acgcgccgga cgaggtgctc
     4501 gggcgcaatc cgcgcatgct cgcctccggg cgccaggatg cagccttcta ccgggccttc
     4561 tggcagtcgc tcgatgcgca cgggctgtgg cagggcgaga tctggaaccg gcgcaagtcc
     4621 ggcgaggtgt ttccggagtg gctgtcggtc agcgtcgtgc gcgatgccga tgccggcccg
     4681 cagcactaca tcgcggtgtt caccgacctc agcgagcaca aggcgcatga gcggcgcatc
     4741 cagttcctcg cgcagtacga cccgctgacg cggctgccga accgggtgct gctgttcgag
     4801 cggctcgaac acgcgctggc ggcggcgcgg cggggcgccc ggcgctgcgc gctgctgttt
     4861 ctcgatctcg accgcttcaa gaacgtcaac gattcgctcg gccacggcat cggcgaccgg
     4921 ctgctcgaag ccgtcgcgca gcgcctgcgc gcggcgctgc gcgactgcga caccgttgcc
     4981 cggctcggcg gcgacgagtt cgtcgtgctg ctcgaagacc tgccggccgg cgacggcgaa
     5041 cgcgaggcgg cgctcgtcgc cggcaagctg ctggccgcgc tcgcgcagcc cttccagatc
     5101 gacacgcacg agctgcgcat caccagcagc gtcggcatca gcctgtatcc gggccacggt
     5161 gccgatgccg agacgctgat ccgcagcgcc gacacggcga tgtaccacgc caagcagtcc
     5221 ggccgggaca acttccagtt cttcaccgag gacatgaacg cccgcgtgct cgaacgcctg
     5281 acgctggaga acggcctgcg ccgggcgctg gcgcagcagg gcttcgcgct ggtctggcag
     5341 ccgcagctcg cgatcggcga cggccggctg atcggcttcg aggcgctgct gcgctggccg
     5401 cagccggagg gacgggcgca ggtgccgccg gcgcagttca ttccggtggc cgaggactgc
     5461 ggactgatcg gcgcgatcgg cgactgggtg ctcgatgccg cctgccggca gctctgcgcg
     5521 tggcgggccg ccggcctgcc gggggtgcgc attgccgtga acctctcggc gctgcagctg
     5581 cgcgatgccc ggctggccga caaggtggcc gcggccctcg ccgtctgcgg gctggacggt
     5641 gccgcgctgg agctcgaagt caccgagagc gcgctgatga gcgatccgcc gcagaccgcc
     5701 ggcacgctgg cacggctgcg cgcgctcggc gtgcagctcg cgatcgatga cttcggcacc
     5761 gggtattcgt cgctcgcata tctgaagcag tttccgatcg accgcctgaa gatcgatcgc
     5821 agcttcgtgc gtgatctcgc cagcgatccg aacgatgcag ccatcgcgcg cacgatcatc
     5881 acgctcgcgc acagcctggg cctgcgcgtg atcgccgagg gcgtcgagac gccggcgcag
     5941 ctcggattcc tccgcggcca cggctgcgac gaggcgcagg gctatctgta cgcgccgccg
     6001 ctcagtccgg agcaggctgc cgaggtgctc gccgccggcc ggatccggcc ggcggcgagc
     6061 accggcaccg atgccgggcc ggcctgatgc cgctcacacc ggcaggcttc gtccttcagc
     6121 ccctcgtccg gcgcgggaga ggggggagaa aaaccatgcg cttgcgtgtg atcggcctgc
     6181 cgtgcgcggc gggcgctcag cggtcgcgtt tctcgtgcag cacgacgagc gcgagccccg
     6241 cgaagccgat cacgaagccg aagggcacca gcgtcaccag cgtcatgccg gtgtcggcgt
     6301 gaccggcgag catcaggccg aagccgccga gcaggccggc gacgatcagc agcagcgcga
     6361 ggcgcagcgt ccattgtccg atcctgagca tggctgacgg gtctttggca ggggaggggc
     6421 cggcgagctt agcgcgcttt gccgcggcct gccgtcacgg cgggccgatg cggccgatgc
     6481 cgggcagctt cagcgccggt ccgcccggat cgatgccggc ggtcaggccg agcaggtcga
     6541 tctccaggcc ctcctcgacg gcgagcgtca gaccggcgag gccgccgagc gtcagctgcc
     6601 agccggtgcc gctcggggcc ggggccagcc agcggccgtc gaccgggtaa tccttgccga
     6661 tcgccgtcgc cggcagcgcc agacgcagct ccggcaccgc gcgcgcgagc tgggcgacga
     6721 aggtgttgct gttcggaccg ggccagaccc ggtagcgatc ggcgtaggga taacgccgtg
     6781 cggcatcgag caggcgcggg atggcgtcgg ccgcggccgc gccgcgcagc tcggcgagca
     6841 gcagcgggcg gttgccgtac cagtaggcgt ccgggacgcc gctcgtgatg ctgaccgccg
     6901 agccgccgcg gtacagccgc cagccgatca cctccagccg ctgccaggcc ggcgccccgg
     6961 ccggtttcac ggcgatccag gtatgcacgc cgaagatgcc gcgccagccc caggcgcgcg
     7021 ctgcccaggc ctgcacgatg gcttcgggct ggcgcccggc ctcgggcacc agccgggcgc
     7081 tgtcgtggcg ggccgcgtac cagggctgat ccgttgcgct cagcgcatga cgtgcggcct
     7141 gcacgacaac cggaaacacg aatacgagca gcaaccagag catcgggcga cggtaccagc
     7201 ggcgggtgcg gcggcggggc ttgccgccgg cgggttcttc gggatcggca gcggaagtgg
     7261 caggcagatc ggacatggcg gcggcgggtt ctgaaagcgg ggggcggaga cggttcagtg
     7321 tgtagccggt gtgcatccac ggtgtatccg gcgtgcatcc gatgtgtatc gggtggacgc
     7381 cggaatcctg accatgcagc ggcgcgcggc gccggtcttt ccctgttgtt gcagaaagat
     7441 ggcgttaaca gcaagaaatc ctgatgcgac ccctagaaat gctggtttgt cgctgcgaaa
     7501 ccccgtccgc acaatcactt ccggcatctt cgtgatgttt ctcctcccat cctcctctgg
     7561 tggtcgtgtt gggccgggtc gtcggacccg
//
LOCUS       DAOQIE010000195         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5069_length_7578_cov_9.737339, whole genome shotgun sequence.
ACCESSION   DAOQIE010000195 DAOQIE010000000
VERSION     DAOQIE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5069_length_7578_cov_9.737339"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..283
                     /locus_tag="PK144_12255"
     tRNA            207..283
                     /locus_tag="PK144_12255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:241..243,aa:Val,seq:gac)
     gene            401..2326
                     /gene="thrS"
                     /locus_tag="PK144_12260"
     CDS             401..2326
                     /gene="thrS"
                     /locus_tag="PK144_12260"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNF66545.1"
                     /translation="MPVITLPDGSRREFEQPVTVLEVARSIGAGLARAALAGRVDGRL
                     VDLSHVIAGDAALALITERDAEGLDVIRHSSAHLLAHAVKQLYPAAQVTIGPVIENGF
                     YYDFAFERPFTPEDLAQIEKRMEELAARDIAVERYELPRDEAVAFFRAQGEEYKAQII
                     ERIPANEALSLYRQDDFTDLCRGPHVPSTGRLKAFKLMKVAGAYWRGDSKNEMLQRIY
                     GTAWTDRKALKDYLHRLEEAEKRDHRRLAKQLDLFHIQDEAPGMVFWHPNGWTIWQAV
                     EQYVRGRLRTAGYVEVRTPQVVDRSLWERSGHWENYSELMFTTSSESRDYAVKPMNCP
                     CHVQIFNHGLHSYRDLPLRMAEFGSCHRNEPSGALHGLMRVRGFTQDDAHIFCTEDQI
                     IAEAQAFNTLVQQVYADFGFTDVAIKLALRPDKRAGSDEVWDRAEDGLRQALTACGLE
                     WQELPGEGAFYGPKVEYHIRDAIGRSWQCGTLQLDFVLPGRLGAEYVAEDNTRKVPVM
                     LHRAVLGSMERFIGILIEHHAGQMPTWLAPQQAVVLNITDRAAEAATAAAARLNALGH
                     RAEADLRNEKIGFKIREHTLRRIPYLLVIGDREVETGSVAVRTRTGKDLGVMTLGSFE
                     EFLAADVASRGRSVLEG"
     gene            2330..2848
                     /gene="infC"
                     /locus_tag="PK144_12265"
     CDS             2330..2848
                     /gene="infC"
                     /locus_tag="PK144_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNF66546.1"
                     /translation="MAANNEIRVNDEITAREVRLIDQNGEQAGVMPLFQAKRIAEDAE
                     LDLVEISPTAKPPVCRIMDYGKFRFEQQKKAAEARKKQKQIQVKEVKFRPGTDEGDYQ
                     VKLRNLVRFLTEGDKAKITLRFRGREVVHQDIGLNLLKRIEDDLAEFGTIEQRPKMEG
                     RQMIMVVAPKRK"
     gene            2912..3109
                     /gene="rpmI"
                     /locus_tag="PK144_12270"
     CDS             2912..3109
                     /gene="rpmI"
                     /locus_tag="PK144_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015464854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNF66547.1"
                     /translation="MPKMKTNRGAAKRFARTGSGGFKRSQAFARHILTKKTTKRKRQL
                     REPAMVAAVDVPMVRRMMPYA"
     gene            3142..3501
                     /gene="rplT"
                     /locus_tag="PK144_12275"
     CDS             3142..3501
                     /gene="rplT"
                     /locus_tag="PK144_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNF66548.1"
                     /translation="MPRVKRGVTAHARHKKILDKAKGYQGARSRVFRVAKQAVTKAAQ
                     YAYRDRRQKKRQFRALWIVRINAAARTCGLSYSRLIGGLKKAAIVIDRKVLADMAVFD
                     KAGFAAVAEKAKAALAQ"
     gene            3665..4681
                     /gene="pheS"
                     /locus_tag="PK144_12280"
     CDS             3665..4681
                     /gene="pheS"
                     /locus_tag="PK144_12280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNF66549.1"
                     /translation="MDTLQTIVLAAETAVAAAADLAALDQVRVHYLGKKGVLTEQLKT
                     LGTLAPEARREAGAAINVAKDAVQAALEARRQALETEALHRRLAAERVDVTLPGRGVQ
                     AGGLHPITRTLRRIEELFRQVGFSVAEGPEIEDDFHNFEALNIPAHHPARAMHDTFYF
                     DASTVLRTHTSPVQIRTMRACEPPIRIIAPGRVYRCDSDLTHSPMFHQVEGLFVDEDV
                     SFADLKGVLYDFLRAFFERDLDVRFRPSYFPFTEPSAEVDIQCVICGGSGCRVCKHTG
                     WLEVLGSGMVHPAVFGKVGIDPERYTGYAFGMGVERLAMLRYGVNDLRLFFDNDLRFL
                     GQFR"
     gene            4712..7090
                     /gene="pheT"
                     /locus_tag="PK144_12285"
     CDS             4712..7090
                     /gene="pheT"
                     /locus_tag="PK144_12285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNF66550.1"
                     /translation="MKISEQWLREWTNPAVSTDELVAQLTMAGLEVDAVTPVAGVFSG
                     VVVGHVLDVQPHPDADKLRVCRVDAGSGAPLQIVCGAPNVHTGMRAPVATIGAVLPGD
                     FKIRKSKLRGVESFGMLCSARELGLSEDHGGLMPLPAEAVPGTDLRAYLGLDDRVIEV
                     DLTPNRGDCLSVAGVAREVAVLNGSALTPVACTPVPAASEEVFPVRIEAGADCPRYAG
                     RVIRGVDPRAATPLWMTERLRRAGLRSLGPLVDVTNYVLLELGQPMHAFDLGRLDGGI
                     VVRKAVAGETLALLNGTRIELQPESLVIADHAGPVALAGIMGGEPSSCTDATVDVFLE
                     SAFFAPQRIAGRARRYGLHTDSSHRFERGVDPALQVPAIERATKLILELCGGTPGPVI
                     EVVDDEELPVAPCIRLRRSRIRRLLGLELDDAVITGMLQRLGMQVLAVEDGWHVTPPS
                     FRFDMAIEADLIEDLGRIHGYNRLPTNRPLSRFAIGAIPESRLPLDRLRSVLVDRGYQ
                     EAITYSFVDPVFQSALDPQHAPVALANPISADLAVMRTSLWPGLVKALEYNRKRQQPR
                     VRLFETGLRFLATDAGMVQDSRIGGVVCGEALAEQWGLPARAVDFFDLKGDVEALLAT
                     SGEPGTVRFTAAEHPALHPGQSARILRDGVAIGWIGLVHPAIEKQLDLGGRVYAFELD
                     LTATARARVPAYAELSKFPAIRRDLAVVVAEDVPAGKVHACLRARAGEWLRESWLFDV
                     YRGKGIDENHKSLAFGLILQDFSRTLADADVDSVLSGIIAGLHEEFGATLRV"
     gene            7096..7419
                     /gene="ihfA"
                     /locus_tag="PK144_12290"
     CDS             7096..7419
                     /gene="ihfA"
                     /locus_tag="PK144_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNF66551.1"
                     /translation="MALTKADMAERLFDELGLNKREAKDLVEIFFEEIRGALESGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEEIPITARRVVTFRPGQKLKARVEEYSERVAAEA
                     ERRNA"
     gene            7500..7576
                     /locus_tag="PK144_12295"
     tRNA            7500..7576
                     /locus_tag="PK144_12295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7534..7536,aa:Pro,seq:ggg)
BASE COUNT         1249 a         2599 c         2524 g         1206 t
ORIGIN      
        1 agcaccacct tgacatggtg ggggtcgttg gttcgagtcc aatcgcgcct accagttttt
       61 tcggggacct ccctgaaaac cccgcaaaag cccgtctccg gacgggcttt tgcattgcag
      121 gcggtccggt ccgggcaggt gaattcctca cggatcgagg cttgcatcgc agcggacgca
      181 tccgtaagat gcgccgccta cgctacaggc gcgtagctca gctggttaga gcaccacctt
      241 gacatggtgg gggtcgttgg ttcgagtcca atcgcgccta ccaaatcccg aaagggcgcg
      301 ccgtccggtc gacggcgcgc cctttccgtt tctgccctcg ggcgccccgg tatccgccgg
      361 catcacctgg tcacaaccag aattcggagg cagcgcagcc atgcccgtca ttacgctccc
      421 ggatggcagc cggcgcgaat tcgagcagcc ggtcacggtg ctggaagtcg cccgctcgat
      481 cggtgccgga ctggcgcgcg cggcgctggc cgggcgtgtc gatggccgcc tggtcgatct
      541 gtcgcatgtg atcgccggtg atgcggcgct ggcgctcatt accgagcgcg atgccgaagg
      601 gctcgatgtc atccgtcatt cgagcgcgca cctgctcgcg cacgcggtca agcagctgta
      661 tccggccgcg caggtcacga tcgggccggt gatcgaaaac ggcttctatt acgacttcgc
      721 gttcgagcgt cccttcacgc ccgaggatct ggcgcagatc gaaaagcgca tggaagaact
      781 cgccgcgcgc gacatcgccg tcgagcgcta cgaactgccg cgcgacgagg cggtcgcgtt
      841 cttccgcgcc cagggcgagg aatacaaggc gcagatcatc gagcgcattc cggcgaacga
      901 ggcgctgtcg ctgtaccgcc aggacgattt caccgacctc tgccgcggcc cgcacgtgcc
      961 gtcgaccggc cggctgaagg cgttcaagct gatgaaggtc gccggggcct actggcgcgg
     1021 tgactcgaag aacgagatgc tgcagcgcat ctacggcacg gcctggaccg accgcaaggc
     1081 gctgaaggac tacctgcacc ggctcgaaga ggccgagaag cgcgatcacc gccggctcgc
     1141 gaagcagctc gacctgttcc acatccagga cgaggcgccg ggcatggtgt tctggcaccc
     1201 gaacggctgg acgatctggc aggcggtcga gcagtacgtg cgcggccgcc tgcgcacggc
     1261 cggctacgtc gaggtgcgca cgccgcaggt cgtcgaccgt tcgctgtggg agcgctccgg
     1321 gcactgggag aactacagcg agctgatgtt cacgaccagc tccgagtcgc gcgactacgc
     1381 ggtcaagccg atgaactgcc cgtgccacgt gcagatcttc aaccacggcc tgcattcgta
     1441 ccgcgacctg ccgctgcgca tggccgagtt cggttcctgc caccgcaacg agccctccgg
     1501 cgcgctgcac ggcctgatgc gcgtgcgcgg cttcacgcag gacgatgccc acatcttctg
     1561 caccgaagac cagatcatcg ccgaggcgca ggccttcaac acgctggtgc agcaggtcta
     1621 cgccgatttc ggcttcaccg atgtcgcgat caagctcgcg ctgcgcccgg acaagcgtgc
     1681 cggttccgac gaggtctggg accgcgccga ggacggcctg cgccaggcgc tgaccgcctg
     1741 cggtctcgaa tggcaggagc tgccgggcga gggcgccttc tacgggccga aggtcgagta
     1801 ccacatccgc gacgcgatcg gccgctcctg gcagtgcggc acgctgcagc tcgacttcgt
     1861 gctgccgggc cgcctcggcg ccgagtacgt cgccgaggac aacacgcgca aggtgccggt
     1921 catgctgcac cgcgcggtgc tcggctcgat ggagcgcttc atcggcatcc tgatcgagca
     1981 ccacgccggc cagatgccga cctggctcgc gccgcagcag gccgtggtgc tgaacatcac
     2041 cgaccgcgcc gccgaagccg cgacggctgc cgctgcgcgc ctcaatgcgc tcggccaccg
     2101 tgccgaggcc gacttgagga acgagaagat cggctttaag attcgcgaac acaccctgcg
     2161 ccggattccg tatctgctgg tgatcggcga tcgtgaggtg gaaacgggtt ctgtggccgt
     2221 gcgtacgcgg accggcaagg atctgggcgt catgacgctc ggctccttcg aggaattcct
     2281 cgctgccgat gtcgcgagcc gcggccgttc tgttttggag ggctgactca tcgctgcgaa
     2341 caacgaaatt agggtgaacg acgagatcac cgcccgcgaa gtgcgtctga tcgatcagaa
     2401 cggcgaacag gcgggtgtga tgccgctctt ccaggccaag cggatcgccg aagacgctga
     2461 actcgatctg gtcgaaatct cgccgacggc aaaaccgccg gtctgccgga tcatggatta
     2521 cggcaaattc cgtttcgagc agcagaagaa ggcagcggaa gcccgcaaga agcagaaaca
     2581 gattcaggtc aaggaagtga aattccgtcc cgggacggac gaaggggatt accaggtcaa
     2641 actacgcaac ctggtacgtt tcctgaccga aggtgacaag gccaagatca cgctgcgctt
     2701 ccgcgggcgt gaggtcgtgc accaggacat cggactgaac ctcctgaagc gcatcgagga
     2761 cgacctcgcc gaattcggca cgatcgaaca gcggccgaaa atggaaggcc gccagatgat
     2821 catggtcgtt gcgccgaagc ggaagtgatt ccgcttctgg ccgatgcgcg gttgcagtcc
     2881 gcaatcaatc aatgaatgcg gagtggctga catgcccaag atgaagacga accgcggcgc
     2941 cgcgaagcgt ttcgcgcgca ccggttccgg tggtttcaag cgttcccagg cctttgcccg
     3001 tcacatcctg acgaagaaga cgacgaagcg taagcgccag ctgcgcgagc cggcgatggt
     3061 cgcggccgtc gacgtgccga tggtgcgtcg catgatgccg tacgcctgag tcgtcacgat
     3121 ccggactaga ggacaaagac catgccccga gtgaagcgcg gcgttaccgc ccacgcccgt
     3181 cacaagaaga tcctggacaa ggccaagggc taccagggtg cccgttcccg cgtgttccgc
     3241 gttgccaagc aggccgtcac caaggccgcc cagtacgcgt accgcgaccg tcgccagaag
     3301 aagcgccagt tccgcgcgct gtggatcgtg cgcatcaacg ccgcggcccg cacctgcggc
     3361 ctgtcgtaca gccggctgat cggtggcctg aagaaggccg cgatcgtcat cgaccgcaag
     3421 gtcctcgccg acatggcggt gttcgacaag gccggttttg ccgccgtcgc ggaaaaggcc
     3481 aaggccgccc tggcgcagtg accgaaaaag ccgccgtcgc cgtctgcgtg acggcggacg
     3541 gatcgcgaaa gggaaggggg tcttgagctc ctttcccttt tttgttgctt gttgagcatc
     3601 gccggaacgc ggagccgggc gcgtgcgcgc accgccgcgg tttccccccg aacggagctg
     3661 ccccgtggat accctgcaga ccatcgtgct cgcggccgaa accgccgtcg ccgccgctgc
     3721 cgaccttgcc gcgctcgatc aggtgcgcgt gcattacctc ggcaagaagg gcgtgctgac
     3781 cgaacagctg aagacgctcg gcacgctcgc gcccgaggcg cggcgcgagg ccggtgccgc
     3841 gatcaacgtc gccaaggacg ccgtgcaggc cgcgctcgaa gcgcgccgcc aggcgctcga
     3901 aaccgaggcc ctgcaccggc gcctcgccgc cgagcgcgtc gacgtgacgc tgccggggcg
     3961 cggcgtgcag gccggcggcc tgcatccgat cacgcgcacg ctgcgccgca tcgaggagct
     4021 gttccgtcag gtcggcttct cggtcgccga aggcccggag atcgaggacg acttccacaa
     4081 cttcgaggcg ctgaacatcc ccgcgcacca cccggcgcgc gcgatgcacg acaccttcta
     4141 tttcgacgcc tcgacggtgc tgcgcacgca cacctcgccg gtgcagatcc gcacgatgcg
     4201 cgcctgcgag ccgccgatcc gcatcatcgc gcccgggcgc gtctaccgct gcgacagcga
     4261 cctgacgcat tcgccgatgt tccaccaggt cgagggcctg ttcgtcgatg aggacgtgag
     4321 cttcgccgac ctcaagggcg tgctctacga cttcctgcgc gccttcttcg agcgcgacct
     4381 tgacgtgcgc ttccgcccct cgtacttccc gttcaccgag ccctcggccg aggtcgacat
     4441 ccagtgcgtg atctgcggcg gcagcggctg ccgggtctgc aagcacaccg gctggctgga
     4501 ggtgctcggc agcggcatgg tgcatccggc cgtgttcggg aaggtcggca tcgacccgga
     4561 gcgctacacc ggttatgcgt tcggcatggg cgtcgagcgt ctggcgatgc tgcgctacgg
     4621 cgtcaacgac ctgcgcctgt tcttcgacaa cgatctgcgc ttcctcggcc agttccgctg
     4681 aagccgtgcg ccgcccggag gccgaaaacc catgaaaatc agtgaacagt ggctgcggga
     4741 gtggacgaat cccgccgtgt cgaccgacga gctggtcgcg cagctgacga tggccggtct
     4801 ggaggtcgat gccgtcacgc cggtggccgg ggtgttcagc ggtgtcgtcg tcggccacgt
     4861 gctcgacgtg cagccgcacc ccgatgccga caagctgcgc gtctgccgcg tcgatgccgg
     4921 cagcggcgcg ccgctgcaga tcgtctgcgg cgcgccgaac gtgcacaccg gcatgcgcgc
     4981 gccggtcgcg accatcggcg ccgtgctgcc cggtgatttc aaaatcagga agagcaagct
     5041 gcgcggcgtc gagtccttcg gcatgctctg ctcggcgcgc gaactcggcc tgtccgaaga
     5101 ccacggtggc ctgatgccgc tgccggccga ggccgtgccc ggcaccgacc tgcgtgcgta
     5161 cctcggcctc gacgaccggg tcatcgaggt cgacctgacc ccgaaccgcg gcgactgcct
     5221 gagtgtcgcc ggcgtcgcac gcgaggtcgc ggtgctgaac ggctcggccc tgacgccggt
     5281 cgcctgcacg ccggtgccgg ccgcaagcga agaggttttc ccggtgcgca tcgaggccgg
     5341 ggccgactgc ccgcgctacg ccgggcgcgt gatccgtggt gtcgatccgc gggccgcgac
     5401 gccgctgtgg atgaccgagc gcctgcgccg cgccggcctg cgctcgctcg ggccgctggt
     5461 cgatgtcacc aactacgtgc tgctcgaact cggccagccg atgcatgcct tcgatctcgg
     5521 ccggctcgac ggcggcatcg tcgtgcgcaa ggccgtggcc ggcgagacgc tggcgctgct
     5581 gaacggcacg cgcatcgagc tgcagcccga atcgctggtg atcgccgatc acgccggccc
     5641 ggtggcgctc gccggcatca tgggcggcga gccgagctcg tgcaccgacg ccaccgtcga
     5701 tgtgttcctg gagagcgcct tcttcgcgcc gcagcggatc gccgggcgcg cgcgccgcta
     5761 cggcctgcac accgattcct cgcaccgctt cgagcgcggc gtcgatccgg cgctgcaggt
     5821 gccggcgatc gagcgcgcga cgaagctgat cctcgaactc tgcggcggca cgccggggcc
     5881 ggtcatcgag gtcgtcgatg acgaggaact gccggtcgcc ccgtgcatcc gcctgcgccg
     5941 ttcccgcatc cgccgcctgc tcgggctcga actcgacgat gccgtcatca ccggcatgct
     6001 gcagcgcctc ggcatgcagg tgctcgcggt cgaggacggc tggcacgtga cgccgccgtc
     6061 cttccgcttc gacatggcga tcgaggccga cctgatcgag gacctcggcc gcatccacgg
     6121 ctacaaccgc ctgccgacca accggccgct gtcgcgcttc gcgatcggcg cgatcccgga
     6181 atcacgcctg ccgctcgatc ggctgcgcag cgtgctggtc gatcgcggct atcaggaagc
     6241 catcacctac agcttcgtcg atccggtctt ccagagcgcg ctcgatccgc agcacgcacc
     6301 ggtggcgctt gccaacccga tctcggccga tctggcggtg atgcgcacca gcctgtggcc
     6361 gggcctggtc aaggcgctgg agtacaaccg caagcgccag cagccgcgcg tgcgcctgtt
     6421 cgagaccggc ctgcgcttcc tcgccaccga cgccggcatg gtccaggaca gccgcatcgg
     6481 cggcgtggtc tgcggcgagg ccctggccga gcagtggggc ctgccggcgc gcgccgtcga
     6541 cttcttcgac ctcaagggcg atgtcgaggc gctgctcgcc accagcggcg agccgggcac
     6601 ggtccgcttc accgcggccg agcacccggc gctgcatccg ggccagagcg cacgcatcct
     6661 gcgcgacggc gtcgcgatcg gctggatcgg cctcgtgcac ccggcgatcg agaagcagct
     6721 cgacctcggc gggcgtgtct acgccttcga actcgatctg acggcgaccg cgcgggcacg
     6781 cgtgccggcc tatgcggagc tgtccaagtt ccccgccatc cgccgcgacc tcgccgtcgt
     6841 cgtcgccgag gacgtgcccg ccggcaaggt gcacgcctgc ctgcgcgcgc gtgccggcga
     6901 gtggctgcgc gagagctggc tgttcgatgt gtatcgcggc aagggaattg atgaaaatca
     6961 taagagcctt gctttcggct tgattctgca ggatttttca cgcacgcttg ccgacgctga
     7021 cgtagactcc gttctttccg ggatcattgc cggactccac gaagagttcg gcgccacgct
     7081 tagggtttga cgctcatggc actgaccaag gccgacatgg ccgaacgact gttcgacgaa
     7141 ctgggcctca acaagcgcga ggccaaggat ctggtcgaga tattcttcga ggagattcgc
     7201 ggtgcgctcg aatcgggcga gcaggtgaag ctctccggtt tcggcaactt cgacctgcgc
     7261 gacaagaacc agcgtccggg ccgcaacccg aagaccggcg aggaaatccc gatcaccgcc
     7321 cgccgcgtcg tgaccttccg ccccggccag aagctcaagg cccgcgtcga ggaatactcc
     7381 gagcgcgtcg ccgccgaggc cgagcgccgc aacgcctgaa cctccggcgc gcggcttgca
     7441 tcgcgaaggg gctccggtag aatgcggacc cttcccgcgc cacggcgcgg accgcatcac
     7501 ggggcgtagc gcagcctggt agcgcacctg catggggtgc aggtggtcgg aggttcaaat
     7561 cctctcgccc cgaccaat
//
LOCUS       DAOQIE010000196         7518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5127_length_7518_cov_8.064049, whole genome shotgun sequence.
ACCESSION   DAOQIE010000196 DAOQIE010000000
VERSION     DAOQIE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5127_length_7518_cov_8.064049"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..681)
                     /locus_tag="PK144_12300"
     CDS             complement(118..681)
                     /locus_tag="PK144_12300"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HNF66552.1"
                     /translation="MPSLPEGIVAGHAARLLARAATPERFDARLFARLLGARIAAGNI
                     EALGLNSVALARLYGHWFPNAAAPRVTQPAPVPAHRLDEVDDVRVLLLEYRSRQTEEE
                     IWLADILAHACLGQNHLWEDLGLADRAQLSELLKRHFAPLAAKNTGGMRWKKFFYKQL
                     CEREGLNLCRAPSCAVCAEHPVCFGPE"
     gene            complement(665..949)
                     /gene="fdxB"
                     /locus_tag="PK144_12305"
     CDS             complement(665..949)
                     /gene="fdxB"
                     /locus_tag="PK144_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HNF66553.1"
                     /translation="MSAPLTTRDGRDWQPQYVASIDQETCIGCGRCYKVCSRNVLALM
                     GLNEDDEWVDPQDDEAERKVMTIADAGDCIGCQACSKVCPKGCYTHAVAA"
     gene            complement(946..1152)
                     /locus_tag="PK144_12310"
     CDS             complement(946..1152)
                     /locus_tag="PK144_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HNF66554.1"
                     /translation="METAEELKALVKTLNGRATKLKMDLHDLSEDLPTGWEKIPEVAQ
                     AAFDAYRELTDARARLAAAKETSA"
     gene            complement(1192..1623)
                     /locus_tag="PK144_12315"
     CDS             complement(1192..1623)
                     /locus_tag="PK144_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HNF66555.1"
                     /translation="METLFVRELVKLLRAQDTYGTWEGKADEALLGPYVLDAEARRAI
                     PIIGDPDPETLWRLEIFHDAMGLAIARETGVMVSAMMKMSHEGFGRVVLIAGRLVVLN
                     RHLRDVHRFGFPSLEKLAAEGMKHVGAAVEMIRKYPEVANT"
     gene            complement(1660..2061)
                     /gene="nifX"
                     /locus_tag="PK144_12320"
     CDS             complement(1660..2061)
                     /gene="nifX"
                     /locus_tag="PK144_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HNF66556.1"
                     /translation="MKVGFTTQDLKRVDAHFGWAKNLAIYEVSTEGYQFLEAIQFGGK
                     LEEDGDEDKLTPKVEAVKDCAILYVAAIGGSAAARVVAARVHPIKVADAPAITDLLDK
                     LREVLQGVPPPWLRKALQKGQERSFDFAEET"
     gene            complement(2105..3460)
                     /gene="nifN"
                     /locus_tag="PK144_12325"
     CDS             complement(2105..3460)
                     /gene="nifN"
                     /locus_tag="PK144_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HNF66557.1"
                     /translation="MAKIIHGKKACAVNPLKMSQPLGASAAFLGIDGCMPVMHGSQGC
                     TSFGLVLLVRHFRETIPLQTTAMTEVATVLGGLENLEAAILNIHKRASPRIIGICSTG
                     VTETKGDDVDGFLKNFRAKHPELDGLALVYVSTPDFSGAFQDGWAKCVSAMIRTLTRP
                     AAERVEKRINVLAGAHLTVADIEEIRETIEAFGLEAVMLPDLSGSLDGHIPETFLPHT
                     LGGTPVEAIENLGSAAFTIAIGAQMAESAALLGTKTGVPYTVFDRLTGLDASDRFMTC
                     LSELSGRPVPDKYRRQRSQLCDAMLDGHFFFGNKRTVIGAEPDLLWALGNFLTEMGCQ
                     LVGAVTTTDSAQLAELPCDEVLIGDLEDLETLARERHAELMVTHSHGRQAAERLGIAF
                     HRAGLPLFDRIGAAHKLSVGYRGTRDLVFEVGNLLISHMPHHHPDDWVNPEAGHVHHA
                     S"
     gene            complement(3464..4852)
                     /gene="nifE"
                     /locus_tag="PK144_12330"
     CDS             complement(3464..4852)
                     /gene="nifE"
                     /locus_tag="PK144_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HNF66558.1"
                     /translation="MSTLTQKVQDVFNEPGCDHNQSKSAKERKKGCTKQLTPGAAAGG
                     CAFDGAKIALQPITDVAHIVHGPIACEGNSWDNRHAASSGPTLYRTGFTTDINELDVI
                     YGGEKRLFKAIREAIEKYDPPAVFVYQTCVTAMIGDDIEAVCRAAREKFGKPVIPVNA
                     PGFVGAKNLGNKLAGEALLEHVIGTEEPDYVTPYDINIIGEYNLVGELWQVRPLLDKL
                     GIRILSCISGDAKYREVAYSHRAKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGVS
                     DTSESLRNIAKLLVERGADAGLIERTEALIAEEEARVWQQLAPFKPRFEGKRVLLMTG
                     GVKSWSVVAALQEAGLEIAGTSVKKSTKEDKDRIKEIMGTDAHMFEEMPPREMYKILR
                     DAKADIMLSGGRSQFVALKAKTPWLDINQERHYAYAGYVGIIELVREIDKALFNPVWE
                     QVRRPAPWEKEV"
     gene            5125..5373
                     /locus_tag="PK144_12335"
     CDS             5125..5373
                     /locus_tag="PK144_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66559.1"
                     /translation="MRRLTGSLMLLAAHTAFAGGAYLKQDITGRWHYPELHPVQISAE
                     EAAGDSRGDEHAHANREPTRTVIAPPAAQPPAPATKPR"
     gene            5637..5870
                     /locus_tag="PK144_12340"
     CDS             5637..5870
                     /locus_tag="PK144_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66560.1"
                     /translation="MTYVYQCHPSRLPQGPYGQMLEELTEARRLGLPASERVAIITRY
                     RALWGCMEDGPVHCQRGPDCIDCFLRHLLSQDY"
     gene            5998..6552
                     /locus_tag="PK144_12345"
     CDS             5998..6552
                     /locus_tag="PK144_12345"
                     /inference="COORDINATES: protein motif:HMM:NF019000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HNF66561.1"
                     /translation="MGHPKPPVTARRNGVRLEARRQWPVPQTREEAIEAIAAIGRHQR
                     ERQRLEAAMNEELAAVRARHEAEAAPHTAAIDGLSHAVEVWADANRALLTRHGRTRSA
                     KLASGDIGWRLSPPAVEVEGEAAVLEELQRRGLQRFVRVRESLNREAVLHEPEAVADV
                     PGIEVVQHEAFVIKPYATPLDESA"
     gene            6675..7454
                     /locus_tag="PK144_12350"
     CDS             6675..7454
                     /locus_tag="PK144_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017008488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNF66562.1"
                     /translation="MLDLFSADPSLLLMFAVVSFTAGFVDAIAGGGGLLTVPALLSAG
                     IPPHLALGTNKLSATFGSSTASFAFVRKGLFRPADWRAAALATLIGAGFGALLTRAFS
                     ADVLKTLLPLSVLGAALYLVWPRRRLRRGEPDPAFRASGRSQWTAGLGIGFYDGFIGP
                     GTGAFWMAVAVKYFRQDLVRGAGLARFMNFLSNAAALLMFALLGNIDWALGLAMGPAL
                     MLGAWLGAHSAIRYGSPLIRPMFIAVVVAMTIRLLWVQFAH"
BASE COUNT         1134 a         2582 c         2520 g         1282 t
ORIGIN      
        1 tcgaagcgca cgttcagcag gaattcgcgc cgccgcgcct gcaggcgaac gtcgaaatcg
       61 agcgtgagcc gcgctgccgg caggatcggg gtcgggagcg cggcggacac ggtgacctca
      121 ctccggtccg aagcagaccg ggtgttcggc gcagaccgca cagctcgggg cgcggcagag
      181 gttcagcccc tcgcgctcgc agagctgctt gtagaagaac ttcttccagc gcatgccacc
      241 ggtgttcttc gcggccagcg gcgcgaagtg acgcttcagc agctccgaga gctgcgcgcg
      301 gtcagccagg ccgaggtctt cccacagatg gttctggccg aggcaggcgt gcgcgaggat
      361 gtcggcgagc cagatttcct cttcggtctg acggctgcgg tattcgagca gcagcacgcg
      421 cacgtcatcg acctcgtcga ggcgatgcgc cggcaccgga gccggctgcg tcacgcgcgg
      481 agcggccgcg ttcgggaacc agtggccgta gaggcgcgcg agcgcgacgg aattgaggcc
      541 gagcgcctcg atgttgccgg ccgcgatccg cgccccgagc aggcgtgcga acagccgcgc
      601 gtcgaagcgc tcgggcgtcg ccgcgcgcgc aagcaggcgc gcggcgtggc cggcgacgat
      661 gccttcaggc agcgacggca tgggtgtagc agcccttcgg gcagaccttc gagcaggcct
      721 ggcaaccgat gcagtcaccg gcgtcggcaa tggtcatgac cttgcgctcg gcctcgtcgt
      781 cctgcggatc gacccattcg tcgtcctcgt tcagacccat cagggccagc acgttgcgcg
      841 agcagacctt gtagcagcgg ccgcagccga tgcaggtctc ctggtcgatc gaggcaacgt
      901 actgcggctg ccagtcacgg ccgtcgcggg tggtcagggg ggcgctcatg cggaagtctc
      961 cttggcagcg gcgaggcggg cgcgggcatc ggtcagctcg cggtaggcat cgaaggcggc
     1021 ctgggcgact tccggaatct tctcccagcc ggtcggaagg tcttcggaaa gatcatgcag
     1081 gtccatcttc agcttcgtgg cgcggccgtt cagggtcttg accagcgcct tgagttcttc
     1141 ggcggtttcc atctcggggt cctcgtcggg gcgtgcaggg gctgttccgc gtcaggtgtt
     1201 cgcgacttcc gggtacttgc ggatcatctc gacggcggcg ccgacgtgct tcatgccttc
     1261 ggcggcgagc ttctcgagcg acgggaagcc gaagcggtgc acgtcgcgca ggtggcggtt
     1321 cagcaccacc agccggccgg cgatcagcac gacgcggccg aagccctcgt ggctcatctt
     1381 catcatcgcc gagaccatca cgccggtttc gcgggcgatc gccagcccca tcgcgtcgtg
     1441 gaagatctcc aggcgccaca gcgtctccgg gtccggatcg ccgatgatcg ggatcgcgcg
     1501 gcgcgcctcg gcgtcgagca cgtagggtcc gagcagggcc tcgtcggcct tgccttccca
     1561 ggtgccgtag gtgtcctgcg cgcgcagcag cttcacgagc tcgcgcacga acagggtttc
     1621 catcggattc acggcggtgg tttcttcggt gctcacggtt caggtctcct cagcgaaatc
     1681 gaatgagcgt tcctggccct tctgcagggc cttgcgcagc cacggcggcg gcacgccctg
     1741 gagcacctcg cgcagcttgt cgagcagatc ggtgatggcc ggggcatcgg cgaccttgat
     1801 cggatgcacc ctggcggcaa ccacgcgcgc ggcggccgag ccgccgatcg cggcgacgta
     1861 gaggatcgcg cagtccttca cggcctcgac cttcggcgtc agcttgtcct cgtcgccgtc
     1921 ctcttcgagc ttgccgccga actggatggc ttcgaggaac tggtagccct cggtcgagac
     1981 ctcgtagatc gcgaggttct tcgcccagcc gaagtgggca tcgacgcgct tgaggtcctg
     2041 cgtggtgaac ccgactttca tgacatggcc tccgagcggc ggcgtggtgc ggacgatgcc
     2101 gaggctacga cgcgtggtgg acatggccgg cctccgggtt gacccagtcg tccgggtggt
     2161 gatggggcat gtgactgatc agcaggttgc cgacctcgaa caccagatcg cgcgtgccgc
     2221 ggtagccgac gctgagcttg tgcgcagcgc cgatgcggtc gaacagcggc aggccggcac
     2281 ggtggaaggc gatgccgagg cgctcggcgg cctggcggcc gtgcgagtgc gtgaccatca
     2341 gctcggcgtg gcgctcgcgg gccagggttt ccaggtcttc gaggtcgccg atcagcacct
     2401 cgtcacaggg cagttcggcg agctgcgcgc tgtcggtcgt cgtcaccgcg ccgaccagct
     2461 ggcagcccat ctcggtcagg aagttgccga gcgcccacag caggtccggc tcggcgccga
     2521 tgacggtgcg cttgttgccg aagaagaagt gaccatcgag catcgcgtcg cagagctggc
     2581 tgcgctggcg gcggtacttg tccggcaccg gccggccgga gagttcgctc aggcaggtca
     2641 tgaagcggtc gctggcatcg agcccggtca ggcgatcgaa caccgtgtac ggcacgccgg
     2701 tcttcgtgcc gagcagcgcc gccgactcgg ccatctgcgc gccgatcgcg atcgtgaacg
     2761 ccgcggagcc gaggttctcg atcgcctcga ccggcgtgcc gccgagcgta tgcggcagga
     2821 aggtctcggg gatgtggccg tcgagcgagc cggagagatc cggcagcatc accgcttcga
     2881 gcccgaaggc ctcgatcgtc tcgcggatct cctcgatgtc ggcgacggtg aggtgcgccc
     2941 cggcgagcac gttgatgcgc ttttcgaccc gctcggcagc gggccgggtc agcgtgcgga
     3001 tcatcgccga cacgcacttc gcccagccgt cctggaaggc gccgctgaaa tccggcgtcg
     3061 acacgtacac cagcgccagc ccgtcgagct cggggtgctt cgcgcggaag ttcttcagga
     3121 agccgtcgac atcatcgccc ttggtctcgg tgacgccggt cgagcagatg ccgatgatgc
     3181 gcgggctcgc ccgcttgtgg atgttcagga tcgcggcttc gaggttttcg aggccgccga
     3241 gcacggtcgc gacctcggtc atcgccgtgg tctgcagcgg gatggtctcg cggaaatggc
     3301 gcaccagcag caccagcccg aacgaggtgc aaccctgcga gccgtgcatg accggcatgc
     3361 agccgtcgat gccgagaaac gccgccgagg ccccgagcgg ctggctcatc ttcagcggat
     3421 tgaccgcgca ggccttcttg ccgtggatga tcttggccat gattcagacc tccttttccc
     3481 acggcgccgg gcggcgcacc tgttcccaga ccggattgaa cagtgccttg tcgatttcac
     3541 gcaccagttc aatgatgccg acgtaaccgg catacgcgta atggcgttcc tgattgatgt
     3601 cgagccacgg cgtcttggcc ttcagcgcca cgaactgcga acggccgccg gacagcatga
     3661 tgtcggcctt ggcgtcgcgc aggatcttgt acatctcgcg cggcggcatt tcctcgaaca
     3721 tgtgcgcatc cgtgcccatg atttccttga tgcggtcctt gtcctctttc gtcgatttct
     3781 tcaccgaggt gccggcgatc tcgagcccgg cctcctgcag cgccgccacc accgaccagc
     3841 tcttgacgcc gccggtcatc agcagcacgc gcttgccttc gaaacgcggc ttgaacggcg
     3901 ccagctgctg ccagacgcgc gcttcctcct cggcgatcag ggcttcggtg cgctcgatca
     3961 gcccggcgtc ggcgccgcgc tcgaccagca gtttcgcgat gttgcgcagc gactccgagg
     4021 tatcggaaac gccgtagaac gagccctcga agaacgggat gtcgtagcgc tcctccatct
     4081 tgcgcgcgac gttgatcatc gctttcgagc agaccatcat cgcggccttc gcgcggtggc
     4141 tgtaggcgac ctcgcggtat ttcgcatcgc cggaaatgca cgaaaggatg cggatgccga
     4201 gtttgtcgag cagcggccgg acctgccaga gttcgccgac caggttgtat tcgccgatga
     4261 tgttgatgtc gtacggcgtc acgtaatcgg gctcctcggt gccgatcacg tgttcgagca
     4321 gcgcctcgcc ggcgagcttg ttgccgagat tcttggcacc gacgaaaccg ggcgcattca
     4381 ccggaatcac cggcttgccg aacttctcgc gcgccgccct gcacaccgcc tcgatgtcgt
     4441 cgccgatcat cgcggtcacg caggtctggt acacgaacac cgccggcggg tcgtattttt
     4501 cgatcgcttc gcggatcgcc ttgaacagcc gcttctcgcc gccgtagatc acgtcgagtt
     4561 cgttgatgtc ggtcgtgaag ccggtgcgat acagcgtcgg ccccgaggac gccgcatggc
     4621 ggttgtccca ggaattaccc tcgcaggcga tcgggccatg cacgatgtgc gcaacgtcgg
     4681 tgatcggctg cagcgcgatc ttcgcgccgt cgaaggcaca gccacccgcc gcggcaccgg
     4741 gcgtcagctg cttggtgcag cccttcttgc gctccttcgc actcttgctc tgattgtggt
     4801 cgcagccggg ctcattgaag acatcctgga ccttctgcgt cagcgtgctc atcggcgtct
     4861 gtcccgttcg caacggcggg ttaccgtttg cgtggacaga gcaggatcga tgccagtccg
     4921 tgatcctgcc ggatttaccc gccgatacag ccacttgaac cagatttgcc cttgccgggc
     4981 caatggacgg actccggacg atctcccggt ggccgcgtag gttttgtcgc aattcctacg
     5041 tcgcgacgac gaccatggcg aatccggcta cagtcgcggc tccggctgcc gataggccgg
     5101 tacggtccag acgaggaaac ccacatgcgc agactgacgg ggagtctgat gctgctggca
     5161 gcacacaccg cgttcgcagg cggtgcctat ctgaagcagg acatcaccgg gcgctggcat
     5221 tacccggaac tgcatccggt gcagatcagt gccgaggaag ccgcaggcga ttcccgcggc
     5281 gatgagcacg cccacgcgaa ccgcgaaccg acccgcaccg tcatcgcgcc tccggccgcc
     5341 cagccaccgg cgcccgcgac gaaaccgcgc tgacagtgcc gtgcgcgtgc cctgcttggc
     5401 ggatcacctg aaattttctc agcggcgcac gaagaaaccg cttctgacga tggcgacacg
     5461 ccgtgtacgt caatcctgat tgacgccccg gcgcggccgg acgggaatga gcgccgtccc
     5521 tgtcaagccg gcaatacgca gggtcctgcg cctactgcat ctgccgtatc gtcggcaacc
     5581 gtcggggccg tcagcatcgg cagcctgtcc ttcgaccgag tcggccttga cagtccatga
     5641 cttacgttta ccagtgtcat ccctcgcgcc tgccgcaagg cccctacggc cagatgctcg
     5701 aagagctgac cgaagcgcgc cggctcggtc tgccggccag cgagcgcgtc gcgatcatca
     5761 cgcgctaccg cgcactgtgg ggctgcatgg aggacgggcc ggtgcactgc cagcgcggcc
     5821 ccgactgcat cgactgcttc ctgcgccacc tgctgagcca ggactactga cctccccccg
     5881 cccgcgtgcg cggaacattc cgccgcgcca cggctcgcaa ccccaggcat gcaggcaccg
     5941 gcgacccggt gcccctgcat ccccggcagt tgccgcagcc ccgcgaggtc agccgtcatg
     6001 ggacacccga aaccccccgt caccgcccgc cgcaacggcg tgcgcctcga agcccgccgg
     6061 cagtggccgg taccgcagac gcgcgaggaa gccatcgagg ccatcgccgc gatcggtcgc
     6121 caccagcgcg agcgccagcg cctcgaagcg gcgatgaacg aggaactcgc cgccgtgcgc
     6181 gcccgccacg aggccgaggc cgcgccgcac accgccgcga tcgacggcct gagccacgcc
     6241 gtcgaggtct gggccgatgc caaccgcgca ctgctgaccc gccacggcag gacgcgcagc
     6301 gcgaagctcg cgagcggcga catcggctgg cgcctgagcc cgccggccgt cgaggtcgag
     6361 ggcgaagccg cggtgctcga agaactgcag cggcgcggcc tgcagcgctt cgtgcgcgtg
     6421 cgcgagagcc tgaacaggga agccgtgctg cacgagcccg aagcggtcgc cgacgtgccc
     6481 ggcatcgagg tcgtgcagca cgaggccttc gtcatcaagc cctacgcgac cccgctcgac
     6541 gagtccgcgt aaacccggcc ggccgcggcc ggcaccgggc ttcggcagcg gcctccccga
     6601 agcgttataa agccggcccg cagccgccgg cttcccgtcc ggcgcgcgcc ctctcccgct
     6661 gcccggtatt cgtcatcttg gatctcttct cggccgatcc gtcgctgctg ctgatgttcg
     6721 ccgtcgtgtc gttcacggcc ggctttgtcg atgccatcgc cggcggcggc ggcctgctga
     6781 ccgtgccggc gctgctgtcg gccggcatcc cgccgcatct ggcgctcggc accaacaagc
     6841 tcagcgcgac cttcggcagc tcgaccgcct ccttcgcctt cgtgcgcaag ggcctgttcc
     6901 ggcccgcaga ctggcgtgcc gcggcgctcg cgacgctgat cggcgccggc ttcggcgcgc
     6961 tgctgacgcg cgccttcagc gccgacgtgc tgaagacact gctgccgctg tcggtgctcg
     7021 gcgcggcgct gtatctggtc tggccgcgac gccgcctgcg gcgcggggaa ccggacccgg
     7081 cctttcgcgc ctccgggcgc tcgcagtgga cggcgggcct cggcatcggc ttctacgacg
     7141 gcttcatcgg ccccggcacc ggcgcgttct ggatggcggt ggcggtgaag tacttccgcc
     7201 aggacctggt gcgcggcgcc gggctggcgc gcttcatgaa cttcctgagc aacgccgcgg
     7261 cgctgctgat gtttgcgctg ctcggcaaca tcgactgggc gctcgggctg gcgatgggcc
     7321 cggcgctgat gctcggcgcc tggctcggcg cacacagcgc gatccgctac ggcagcccgc
     7381 tgatccgccc gatgttcatc gccgtcgtcg tcgcgatgac gatccggctg ctgtgggtgc
     7441 agttcgcgca ctgaagagaa tgttgccaga ccccgcaagg cattgatctc atgatggact
     7501 tccccctcat gggaaagg
//
LOCUS       DAOQIE010000197         7431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5209_length_7431_cov_8.418655, whole genome shotgun sequence.
ACCESSION   DAOQIE010000197 DAOQIE010000000
VERSION     DAOQIE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5209_length_7431_cov_8.418655"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..873)
                     /locus_tag="PK144_12355"
     CDS             complement(4..873)
                     /locus_tag="PK144_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF66563.1"
                     /translation="MTPSASNPKRALSVLVTGCSSGIGHCAAHGLRARGHRVFAAARR
                     SADVERLRAEGLEAVMLDLDDDAGIGSGLDAVLDATGGRLDALFNNGAYGQPGAVEDL
                     TRAALREQFETNLFGTAVLTNRVIPVMRRQGHGRIVMNSSLLGYAAMAYRGAYNASKF
                     ALEGLTDTLRLELAGSGIHVSLIEPGPITSRFRANAEAAFHRHIDPAGSVHRAAYVAM
                     AERLARPGPAAPFTLGPEAVLDKLVHALESPHPRRRYPVTLPAHAFWWLRRLLPFCLI
                     DRILAAAGGSGRR"
     gene            complement(925..1452)
                     /gene="mobB"
                     /locus_tag="PK144_12360"
     CDS             complement(925..1452)
                     /gene="mobB"
                     /locus_tag="PK144_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNF66564.1"
                     /translation="MLHPSVRPCLGFAACSGTGKTTLLLELIPRLVAAGLRLGLAKHS
                     HHALDLDRTGKDSQRLRMAGAAQVVVAAPRRLARIEACSRNLAECLALLARAPAAPDL
                     ILVEGWKHAPIPRLELHRPALGQPLLAPADPLILAVACDAPPHTPAGVPLLPLGDADA
                     IAACVLGWLAAGRLG"
     gene            1493..2428
                     /gene="xerD"
                     /locus_tag="PK144_12365"
     CDS             1493..2428
                     /gene="xerD"
                     /locus_tag="PK144_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNF66565.1"
                     /translation="MPEQDAGALTAIVPATEETAIEAYIDEVWMEMGLSTNTLAAYRA
                     DLRACAGWLSLRRCGMTLLQAGAADLQAYLAARHTLSPRSLARLQSALRHYYRHQLRH
                     GRIAVDPTGVIETPKLGRRLPGSLSEAEVEALLAAPDAGTSLGLRDRAMLELLYATGL
                     RVSELVTLGRDQVNPRGGVLRVSGKGNKERLVPIGETALDWLERYLAEARPALLGMRL
                     TEALFVTSRGESMTRQNFWYLIKRYAMTAGIDPMRLSPHTLRHAFATHLVNHGADLRV
                     VQLLLGHSDLSTTQIYTHVARERLKRLHERHHPRG"
     gene            2567..3307
                     /locus_tag="PK144_12370"
     CDS             2567..3307
                     /locus_tag="PK144_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNF66566.1"
                     /translation="MLKPLFAALAIALGTATAAAGEAAPAAGPDLTRLSAVLDGRKPD
                     AVNPTPVPGLYEVILGARVLYVSADGRYVLRGDLVDIDKGENLTEARSGQLRVRAIAA
                     VPESDMIVFAPPGPVQAKHTVTVFTDIDCGYCRKLHSEIDNYLRAGIRVRYLAYPRSG
                     LRGESYDKAVSVWCAEDPKAAITSAKKGQAVAKASCPDNPVAKEYELGNQVGVQGTPT
                     IVLENGDLVPGYVPAARLVMMLDHPELR"
     gene            complement(3401..>4295)
                     /locus_tag="PK144_12375"
     CDS             complement(3401..>4295)
                     /locus_tag="PK144_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF66567.1"
                     /translation="EPVANTPMNDPQQTYHFNVGLQTFQPYVIDNSMQVPVLVEFRSG
                     RSEQSRALGATLEKLAAEYHGDFLLAIVDADREVQIAQHFGVRTLPTVLIVFQGQIVD
                     ALAGVQPETEYRRRIDQYRPRPADKVIAESEALWESGEREAAVRRLLEGLRESPAEPD
                     LQVSLAAKLLDLGRYKEAGNILRKLPEELQRMDPVPELLARLEVAEAAPKGIDTVPLE
                     ARLAADPNDHAARLELAEALLGASEHEGAAAQYLELMRRSRAFGDDAGRQGLLKLFEL
                     LGNEHPVTGTFRRRMASLLLN"
     assembly_gap    4296..4395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4396..4709)
                     /locus_tag="PK144_12380"
     CDS             complement(<4396..4709)
                     /locus_tag="PK144_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017429756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNF66568.1"
                     /translation="MTFVVTENCIKCKYTDCVEVCPVDCFHEGPNFLVIDPEECIDCT
                     LCEPECPAEAIFAEDDLPAGQEHFIQLNADLAKDWPVITEQKDPLPDAQDWDGKPGKL
                     GEL"
     gene            complement(4847..7372)
                     /gene="mutS"
                     /locus_tag="PK144_12385"
     CDS             complement(4847..7372)
                     /gene="mutS"
                     /locus_tag="PK144_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF66569.1"
                     /translation="MMQQYLRTRAEHPGVLLFYRMGDFYELFYDDARKAARLLDITLT
                     ARGASAGEPIPMAGVPYHALEGYLAKLVRLGESVAICEQIGDPATSKGPVERKVVRVV
                     TPGTLTDEALLEERRECLLLALHAVKQSWGIATLDLAGGRFVLMQVDGAEALLAELER
                     LQPAELLAAEDEPLPAAVTARAGLRRLPIWHFDGETALRTLSAQFGTQDLAGFGADEL
                     PVAVAAAGALLAYVHDTQKSALPHLQALAVERREDALILDAASRRNLELERSLSGRHE
                     HTLAGVLDVTVTPMGSRLLRRWLARPLRARQPLRLRQHAIGELLGARAHEPLRELLRG
                     VPDVERILARVALKSARPRDLGGLRAALTRLPALRAGADGIDSPRLGELCADFGEHPD
                     TLALLERALVEQPPLLTRDGGMIAAGFDAELDELRALSENADQFLVDLEAKERERTGL
                     NLKLGYNRVTGYYIELTRAAAGTVPVHWHRRQTLKGVERYITPELKAFEDKVLSARER
                     ALAREKALYDGLLDALIAGLGPLQRLAAALAELDALTTLAERAGALDLACPELTDEPG
                     IDIVAGRHPVVEQVSDAPFVPNDLRFDDSRRLLVITGPNMGGKSTYMRQTALIVLLAH
                     IGSYVPAKSARIGPVDRIFTRIGAADDLASGRSTFMVEMTETANILHNATAGSLVLMD
                     EIGRGTSTFDGLSLAWACAHRLAQIGACTLFATHYFELTRLPDEHAGIANVHLDAVEH
                     GEGIVFLHALQDGPASRSYGLAVAALAGMPKPVVTAARRKLKELERVPARRAQLPAEP
                     DPQLPLFAPEPNAVVEALSGIDPDSLSPREALAALYHLKSLLP"
BASE COUNT         1065 a         2489 c         2687 g         1090 t
ORIGIN      
        1 agttcagcgc ctgccgctgc cgccggcggc ggcgaggatg cggtcgatca ggcagaacgg
       61 cagcagccgg cgcagccacc agaacgcatg tgccggtagc gtgaccggat agcggcgacg
      121 cggatgcggg ctttcgagcg catgcacgag cttgtcgagc accgcttccg ggccgagcgt
      181 gaacggggcg gccgggccgg gtctggcgag gcgctcggcc atcgccacgt aggcggcgcg
      241 atgcacgctg cccgccgggt cgatgtggcg atggaaggcg gcttcggcgt tggcacggaa
      301 gcggctggtg atcgggccgg gttcgatcag gctgacgtgg atgccgctgc cggcgagttc
      361 gaggcgcagc gtgtcggtca ggccttcgag cgcgaacttg ctggcgttgt aggcgccgcg
      421 gtaggccatt gcggcatagc cgagcagcga gctgttcatg acgatgcggc cgtgcccctg
      481 gcggcgcatg accggaatca cgcgattggt cagcaccgcg gtcccgaaca ggttggtttc
      541 gaactgctcg cgcagggcgg cgcgcgtgag atcctcgacc gcgccgggct ggccgtaggc
      601 accattgttg aacagcgcat cgagccggcc gccggtggcg tcaagcacgg catcgagacc
      661 cgagccgatg ccggcatcgt catccagatc gagcatgacc gcttccagtc cttccgcccg
      721 cagccgttcg acatcggcgc tgcgccgcgc cgcggcgaac acgcggtgac cgcgtgcgcg
      781 caggccgtgc gcagcacaat ggccgatgcc gctcgaacag ccggtgacga ggacgctgag
      841 tgcgcgcttc gggtttgatg cagatggggt catgctcagc cggcaatacg gtcgggacgg
      901 gaaagttcgt tatgctgccg tgcatcaccc gaggcgaccg gcggcgagcc agcctagcac
      961 gcaggcggcg atggcatccg catcgccgag cggcagcagc ggcacgccgg ccggggtgtg
     1021 cgggggcgca tcgcaggcga cggcgaggat cagcgggtcg gcaggggcga gcagcggctg
     1081 tccgagtgcc ggccggtgca gttcgagtct gggaatcggc gcatgcttcc agccctcgac
     1141 gaggatcagg tccggggcgg ccggcgcgcg cgcaagcagc gccagacatt ccgccaggtt
     1201 gcggctgcag gcctcgatgc gcgccagccg tcgcggtgcg gccaccacca cctgcgcggc
     1261 accggccatc cgcagccgct ggctgtcctt gccggtacgg tcgaggtcga gggcgtggtg
     1321 gctgtgcttg gcgagtccga gccgcaggcc ggccgccacg agccgcggga tcagttcgag
     1381 cagcagcgtt gtcttgcccg tcccgctgca ggcggcgaat ccgaggcagg ggcgcacaga
     1441 cggatgcaac atcagcagga gcaccaggga gatggatgac ggaatcggca gtatgccgga
     1501 gcaggatgcc ggcgccttga ccgcgatcgt gccggcaacg gaagaaacgg cgatcgaggc
     1561 ctatatcgac gaggtctgga tggagatggg cctgagcacc aacacgctcg cggcctatcg
     1621 cgccgatctg cgcgcctgcg ccggctggct gtcgctcagg cgctgcggca tgacgctgct
     1681 gcaggccggc gccgctgacc tgcaggccta tctggccgcc cgccacacgc tgtcgccgcg
     1741 cagcctcgcg cggctgcagt cggcgctgcg gcattactac cgccaccagc tgcgccacgg
     1801 ccgcatcgcc gtcgacccga ccggcgtgat cgaaaccccg aagctcggcc gccggctgcc
     1861 gggctcgctg tcggaggccg aagtcgaggc gctgctggcg gcgccggatg ccggcacctc
     1921 gctcgggctg cgcgatcgcg ccatgctcga actgctgtac gcgaccggcc tgcgcgtcag
     1981 cgagctggtc acgctcggtc gcgatcaggt caatccgcgc ggcggcgtgc tgcgcgtcag
     2041 cggcaagggc aacaaggagc ggctggtgcc gatcggcgag accgcgctcg actggctgga
     2101 gcgctatctc gccgaggccc gtccggcgct gctcggcatg cggctcaccg aggcgctgtt
     2161 cgtgacctcg cgcggcgaat cgatgacgcg gcagaacttc tggtacctga tcaagcgcta
     2221 cgcgatgacc gccggcatcg acccgatgcg cttgtcaccg catacgctgc gccatgcctt
     2281 cgcgacgcat ctggtcaacc atggcgccga cctgcgcgtc gtgcagctgc tgctcgggca
     2341 ttcggatctg tcgacgacgc agatctacac gcacgtcgcg cgcgagcgcc tgaagcgcct
     2401 gcacgagcgc caccacccgc gcggctaggg gcgggccgtc gtccgcggcc gtcattcgac
     2461 cgatgcatcc gcggcagggc agccctgcgt atgtgccggg tgcgcaggcg caggccgcgc
     2521 gttacgctgc cgcccttccc cggatgaccc gatacggaat cccgccatgc tgaaacccct
     2581 gttcgccgcc ctcgcgatcg ccctgggtac ggcgaccgct gccgccggag aggccgcgcc
     2641 cgcggccggt cccgacctga cgcggctcag tgccgtgctc gacggccgca agcccgatgc
     2701 cgtgaacccg acgccggtgc cgggcctgta cgaggtcatc ctcggggcgc gcgtgctgta
     2761 cgtcagcgcc gatggccgct acgtgctgcg cggcgatctg gtcgacatcg acaagggcga
     2821 gaacctgacc gaggcacgca gcggccagct gcgcgtgcgg gcgatcgccg cggtgccgga
     2881 atccgacatg atcgtgttcg cgccacccgg tccggtgcag gccaagcaca cggtgacggt
     2941 gttcaccgac atcgactgcg gctactgccg caagctgcat tcggagatcg acaactacct
     3001 gcgcgccggc atccgcgtgc gctatctcgc ctatccgcgc agcggcctgc gcggcgagtc
     3061 ctatgacaag gccgtcagcg tctggtgtgc cgaggacccg aaggcggcga tcaccagtgc
     3121 caagaagggc caggcggtcg cgaaggcgag ctgcccggac aatccggtcg cgaaggaata
     3181 cgaactcggc aaccaggtcg gcgtgcaggg cacgccgacc atcgtgctcg aaaacggtga
     3241 tctggtgccc ggctacgtgc cggcggcgcg gctggtgatg atgctcgacc atcccgagct
     3301 gcgctgaggc gcggacaggc cgcgcataac cggccggcac gaaaacgggg cctcgcggcc
     3361 ccgttttcat cgggttgact tcatcgggct gacggcggga tcagttcagc agcagcgagg
     3421 ccatgcgccg gcggaaggtg ccggtgaccg ggtgctcgtt gccgagcagc tcgaacagct
     3481 tcagcaggcc ctgacgcccg gcgtcgtcgc cgaaggcgcg gctgcggcgc atcagttcga
     3541 ggtattgcgc ggcggcgccc tcgtgctcgc tggccccgag cagcgcctcg gccagttcga
     3601 gacgggcggc gtggtcgttc gggtcggcgg cgagccgggc ttcgagcggc acggtgtcga
     3661 tgcccttcgg cgcggcctcg gcgacttcga ggcgcgcgag cagctccggc accgggtcca
     3721 tgcgctgcag ctcctcgggg agcttgcgca ggatgttgcc ggcttccttg tagcggccga
     3781 gatcgagcag cttcgcggcc agcgacacct gcaggtccgg ctcggccggg ctctcgcgca
     3841 ggccttcgag caggcggcgc accgccgcct cgcgctcgcc ggattcccac agcgcctcgg
     3901 attcggcgat gaccttgtcg gccggtcgcg gccggtactg gtcgatgcgc cggcggtatt
     3961 cggtctcggg ctgcacgccg gcgagcgcat cgacgatctg gccctggaac acgatcagca
     4021 cggtcggcag cgtgcgcacg ccgaagtgct gcgcgatctg gacctcgcgg tcggcatcga
     4081 cgatcgcgag caggaagtcg ccgtggtact cggcggcgag tttctccagc gtcgcgccga
     4141 gcgcacggct ctgctccgac ctgccggagc ggaactcgac gagcaccggc acctgcatgg
     4201 agttgtcgat gacgtagggc tggaaggtct ggagcccgac gttgaagtga tacgtctgct
     4261 gcggatcgtt catcggcgtg tttgcgactg gctcgnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnagctc gccgagcttg ccgggcttgc cgtcccagtc ctgcgcgtcg
     4441 ggcagcggat ccttctgctc ggtgatcacc ggccagtcct tggcgagatc ggcgttgagc
     4501 tggatgaagt gctcctgacc ggccggcagg tcgtcctcgg cgaagatcgc ctcggccggg
     4561 cattccggtt cgcacagcgt gcagtcgatg cattcctcgg gatcgatgac caggaagttc
     4621 gggccttcgt ggaagcagtc gaccgggcag acctcgacgc agtcggtgta cttgcacttg
     4681 atgcagtttt cggtgacgac gaacgtcatg gcggtatccc tcgttcaaac aacacaagca
     4741 ggcaggcaga caacagacag acgaaaaacc ggtctggatg cggcggcagg ccgcgcagtg
     4801 tacggccggg tgccggcagc tcaaagcgtg ccgggctgcg gccggctcag ggcagcagcg
     4861 atttcaggtg gtagagcgcg gcgagggcct cgcgcggact caggctgtcc ggatcgatgc
     4921 cgctgagggc ctcgaccacg gcattcggtt ccggcgcgaa cagcggaagc tgcggatcgg
     4981 gctcggccgg cagctgcgcg cggcgcgccg gcacgcgttc gagttccttc agcttgcgcc
     5041 gcgccgccgt gaccaccggc ttcggcatgc cggcgagggc ggcgaccgcc agcccgtagc
     5101 tgcggctggc cgggccgtcc tgcagcgcgt gcaggaatac gatgccctcg ccgtgctcga
     5161 ccgcgtcgag atgcacgttg gcgatgccgg cgtgttcgtc gggcaggcgc gtcagctcga
     5221 agtaatgcgt cgcgaacagc gtgcatgcgc cgatctgcgc cagcctgtgt gcgcaggccc
     5281 aggcgagcga caggccatcg aaggtcgagg tgccgcggcc gatctcgtcc atcagcacca
     5341 gcgatccggc ggtggcgttg tgcaggatgt tggcggtctc ggtcatctcg accatgaagg
     5401 tcgagcgccc ggaggcgaga tcatcggccg caccgatgcg cgtgaagatg cgatcgaccg
     5461 ggccgatgcg ggcgctcttc gccggcacgt agctgccgat gtgcgcaagc agcacgatca
     5521 gcgccgtctg gcgcatgtag gtcgacttgc cgcccatgtt cgggccggtg atcaccagca
     5581 gccggcggct gtcgtcgaag cgcaggtcgt tcggcacgaa gggcgcgtcg ctgacctgct
     5641 cgaccaccgg gtggcggccg gcgacgatgt cgatgccggg ttcgtcggtg agttcagggc
     5701 aggccaggtc gagtgccccg gcgcgctcgg cgagcgtcgt cagcgcgtcg agctcggcga
     5761 gggccgcggc gaggcgctgc agcggcccga gtccggcgat cagcgcatcg agcaggccat
     5821 cgtacaaggc cttctcgcgc gccagcgccc gttcgcgggc cgacagcacc ttgtcctcga
     5881 aggccttcag ctcgggcgtg atgtagcgct cgacgccctt gagcgtctgg cgccggtgcc
     5941 agtgcaccgg cacggtgccg gcggcggcgc gcgtcagctc gatgtagtag ccggtgacgc
     6001 ggttgtagcc gagcttcagg ttcaggccgg tgcgttcgcg ctccttcgct tccagatcga
     6061 cgagaaactg atcggcgttc tccgacagcg cgcgcagctc gtcgagttcg gcatcgaaac
     6121 cggcagcgat catgccgccg tcgcgggtca gcagcggcgg ctgctcgacc agcgcgcgtt
     6181 cgagcagcgc gagcgtgtcc ggatgctcgc cgaagtccgc gcacagctcg ccgaggcgcg
     6241 gggagtcgat gccgtccgcg cccgcccgca gcgccggcag gcgcgtgagt gccgcgcgca
     6301 ggccgccgag atcgcgcggg cgtgccgatt tcagcgccac gcgcgcgagg atgcgctcga
     6361 catccggcac gccgcgcagc agctcgcgca gcggttcgtg cgcgcgcgcg ccgagcagct
     6421 cgccgatcgc gtgctggcgc agcctgagcg gctggcgcgc gcgcagcggc cgcgccagcc
     6481 agcggcgcag cagccggctg cccatcggtg tcacggtcac gtcgagcacg ccggccagcg
     6541 tgtgctcgtg gcggcccgac aggctgcgtt cgagttcgag gttgcgccgg ctcgcggcat
     6601 cgaggatcag cgcatcctcg cgccgctcga ccgcgagcgc ctgcaggtgc ggcagcgcgc
     6661 tcttctgcgt gtcgtgcacg taggcgagca gcgccccggc cgcggccacc gccaccggca
     6721 gctcatcggc gccgaagccg gcgaggtcct gcgtgccgaa ctgcgcgctc agcgtgcgca
     6781 gcgccgtctc gccgtcgaag tgccagattg gcagccggcg cagcccggcg cgcgccgtga
     6841 ccgcggccgg cagcggttca tcctcggcgg cgagcagctc ggccggctgc aggcgttcga
     6901 gttcggcgag cagcgcctcg gcgccgtcga cctgcatcag cacgaagcgc ccgccggcca
     6961 gatcgagcgt cgcgatgccc cagctctgct tcaccgcgtg cagcgcgagc agcaggcatt
     7021 cgcgccgttc ttcgagcagg gcctcgtcgg tcagcgtgcc gggcgtgacc acgcgcacga
     7081 ccttgcgctc gaccggcccc ttgctggtcg ccggatcacc gatctgctcg cagatcgcca
     7141 ccgactcgcc gaggcgcacg agcttcgcga gatagccttc gagcgcgtgg tagggcacgc
     7201 cggccatcgg gatcggctcg ccggccgagg cgccgcgcgc ggtcagcgtg atgtcgagca
     7261 ggcgtgcggc cttgcgggcg tcgtcgtaga acagctcgta gaaatcgccc atccggtaga
     7321 acagcagcac cccggggtgc tcggcccggg tgcgcaggta ctgctgcatc atcggcgtgt
     7381 ggcgggcgta cggatcggcg gcagcgacgg tcacggcagg tctggggctc g
//
LOCUS       DAOQIE010000198         7423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5213_length_7423_cov_7.897801, whole genome shotgun sequence.
ACCESSION   DAOQIE010000198 DAOQIE010000000
VERSION     DAOQIE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_7423_cov_7.897801"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK144_12390"
     CDS             <1..274
                     /locus_tag="PK144_12390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF66570.1"
                     /translation="LAPQPLALATAGTTSSGVFLQLGAFGDRRNAEALKTRIAGRFAQ
                     PVTIDGSRNDGLLRVKIGPFQTEPEAERASLMVASLGLGRPRMVID"
     gene            367..1527
                     /locus_tag="PK144_12395"
     CDS             367..1527
                     /locus_tag="PK144_12395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNF66571.1"
                     /translation="MRLSVALRFLLSFVLLFGPGAAALAVDAVPAAPDLPVRAYVLVD
                     YASENVLAGKLADERIEPASITKLMAAYVIYQSIHEGKLKLDDQVLISENAWRTEGSR
                     MFAEVNSRVPADALLMGMVIQSGNDATVALAEHIAGSETTFVDLMNKWAKQLGLTRSH
                     FMNATGLPHPDHYMTAHDIAKLARALIHDFPEQYARYAIKEFTYNNITQHNRNRLLWL
                     DPSVDGVKTGHTSSAGYCLVTSAKREQGRMIAVVLGAKAEKDRVSASQALLNYGFSFY
                     TTRLMHEAGVALAEPRVWLGDAEKLPLGLAEPLGVTVPKGKAEGLKSEIQVNPQIEAP
                     VGRGSVHGKVVVTLDGNTVAEAPLVALQDVAEGSFFSRLLDHILMLFHKLIG"
     gene            1574..1843
                     /locus_tag="PK144_12400"
     CDS             1574..1843
                     /locus_tag="PK144_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNF66572.1"
                     /translation="MSEAPSDSLLEFPCDFPIKAFGEGDDFEALIVGLVRTHAPDLDP
                     ARVRVAASRTGRYIAVTVVVPAHSRAQLDAIYRDISGHARVKMAL"
     gene            1849..2511
                     /gene="lipB"
                     /locus_tag="PK144_12405"
     CDS             1849..2511
                     /gene="lipB"
                     /locus_tag="PK144_12405"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNF66573.1"
                     /translation="MRSWWRRDLGRRAYAPVYAAMKAWTTARTADAGDEVWVVEHPPV
                     FTQGLNGKAEHLLATGDIPVVAVDRGGQVTYHGPGQIVVYPLLDIERAGIGVRTLVDA
                     LEGAVVDLLAARGIAACAKPDAPGVYVDGRKIASLGLRVRRGRCYHGLAVNIDMDLAP
                     FQRINPCGYAGLQMTQLSELCDDFAIQKATDELLHHLASRLGFADPLPLSELPTALSE
                     DA"
     gene            2513..3529
                     /gene="lipA"
                     /locus_tag="PK144_12410"
     CDS             2513..3529
                     /gene="lipA"
                     /locus_tag="PK144_12410"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNF66574.1"
                     /translation="MAELPEPTSETLAPAAGNAPVRRVTVTGEKLRGADKVARIPVKI
                     APTTLETRLKKPEWIRARFPGSPEVQRLKGILRENRLHTVCEEASCPNLGECFGHGTA
                     TFMIMGDICTRRCPFCDVGHGRPNPLDPDEPLNMARTIAAMGLKYVVITSVDRDDLRD
                     GGAAHFAECIRRARELSPALQVEVLVPDFRGRVEIAVDILAACPPDVFNHNLETVPRL
                     YRQARPGADYEGSLDLLEAYKARHPQVATKSGLMLGLGEELDEVRAVMRDLRAHGVDM
                     LTLGQYLQPSLHHLPVARYVTPAEFEQLRVDGLEMGFTHVASSPMVRSSYHADLQAKG
                     EFPG"
     gene            complement(3679..3864)
                     /locus_tag="PK144_12415"
     CDS             complement(3679..3864)
                     /locus_tag="PK144_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66575.1"
                     /translation="MNEEMREIVRRAELELGRELTEREFARYVGNRLSVPFTEVPWLW
                     ASYRARKARLACIAMQG"
     gene            complement(4047..6128)
                     /locus_tag="PK144_12420"
     CDS             complement(4047..6128)
                     /locus_tag="PK144_12420"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNF66576.1"
                     /translation="MAPCRLSLAAALLLSFATVACAATVGDALPPLPKTLPDLSPDTV
                     QSSVDKTVAGLLTQYHYRRVKIDSALSATVFDDYLSALDFSHSFLLASDVAELGQYRD
                     KIDDELRNGRLDAAFAIYNLYQQRLLERTRRISEELTRPFDFRKDETYEADRKKAPWA
                     RTAAELDEIWRKRLKNETLSLMLAGKDEAAARDTLRKRYEGRLRRAAQSGPEDVFQIY
                     MNAVTGAFDPHTSYLSPRNSENFNIQMRLSLEGIGAVLRLEDEQTVVVELVPGGPADL
                     SKQIRPKDRILAVGQGDDGAMTDVVGWRLDDVVDLIRGQRGSVVRLQVVPAQAAAGSP
                     GTVVRIVRNKIQLEEQAAKSQVKTFVRDGVERKVGVITIPTFYIDFAAAQRGDLDYRS
                     TTRDVRRLIEQLQRERVEGIVIDLRQNGGGSLQEAVELTGLFIADGPVVQVRDAKGKV
                     QVENDPDPKQFYSGHLAVLTDRFSASASEIFAAAIQDYGRGVLVGEQTFGKGTVQTLI
                     DLGRFLPQVKDKLGQLKLTIAKFYRINGESTQNRGVLPDVALPPAFATDEVGESAQEH
                     ALPWDEIPPTRYKVAGRVRDGVTELKRLHEARVPHDQPYQDLKHDLEVSMALRKQTTV
                     SLLESKRRTDQEASKSRMRTARGRVIDPDSDDAGDAKKDGPDPFLDETGQILLDWIDL
                     RAGRIAQAPAD"
     gene            complement(6140..7123)
                     /locus_tag="PK144_12425"
     CDS             complement(6140..7123)
                     /locus_tag="PK144_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNF66577.1"
                     /translation="MTIKSHANVGVYVDAANISRNGGSRMQYDVLREFACRDYAEALR
                     LNVYLTFDEDRANSNYTYRERMRAYQAALRELGYKVIEKKVRWFEDESGTRYGKANAD
                     LDMAVDLLLQSQNLDRVVLVTGDGDFVQVVRALQNKGCRVEVIGFDNVSEDLRNEADL
                     YVSGFLIPGLLPARGEDTGVPWGEIGSRVRGYCYHHDENKSFGFMRFLVRSSPYLWKT
                     DSRDPDSPYRTAFFHDSNLPDGFNVMRLPSRHHVFEFTLAEPSGKQPVATDIQLVCPP
                     MAERGWRGSSRHGASSSAAAAASTAAEAEGSAGHDDDQDADDGDHPNGNVA"
BASE COUNT         1147 a         2583 c         2467 g         1226 t
ORIGIN      
        1 cctggcgccg cagccgctcg cgctcgccac cgccggcacc accagcagcg gcgtgttcct
       61 gcagctcggc gccttcggtg accggcgcaa cgccgaggcg ctgaagaccc gcatcgccgg
      121 acgcttcgcg cagccggtca ccatcgacgg ctcgcgcaac gacggcctgc tgcgcgtcaa
      181 gatcgggccg ttccagaccg agcccgaagc cgagcgcgcg agcctgatgg tcgcgagcct
      241 cggcctcggc cggccgcgca tggtcatcga ctgatgcgtt tgcgcgccgc aacaatccgg
      301 ccgccgtccg gcggcgggcg ctagaatccg cccccgtttc ctccgtttcc cggtatcgat
      361 ctgcccatgc gcctgtccgt cgctttgcga ttcctgttgt ccttcgtgct gctgttcgga
      421 cccggcgcag ccgccctcgc cgtcgatgcc gtgcccgccg ccccggatct gccggtccgg
      481 gcttacgtgc tcgtcgacta cgccagcgag aacgtgcttg ccggcaagct cgccgacgaa
      541 cgcatcgagc cggccagcat caccaagctg atggctgcct acgtgatcta ccagtcgatc
      601 cacgagggca agctcaagct cgacgatcag gtgctgatca gcgagaacgc ctggcgcacc
      661 gaaggctcgc gcatgttcgc cgaggtcaac tcgcgcgtgc cggccgatgc gctgctgatg
      721 ggcatggtca tccagtccgg caacgacgcg accgtcgcac tcgccgagca catcgccggc
      781 tccgagacga ccttcgtcga cctgatgaac aagtgggcga agcagctcgg cctcacacgc
      841 tcgcacttca tgaacgcgac cggcctgccg catccggatc actacatgac ggcgcacgac
      901 atcgccaagc tcgcgcgcgc gctgatccat gacttccccg agcagtacgc gcgctatgcg
      961 atcaaggaat tcacgtacaa caacatcacc cagcacaacc gcaaccgcct gctgtggctc
     1021 gacccgagcg tcgatggcgt caagaccggc cacaccagca gcgccggcta ctgcctcgtg
     1081 acctcggcca agcgcgagca gggacgcatg atcgcggtcg tgctcggggc caaggccgag
     1141 aaggaccggg tcagtgccag tcaggcgctg ctgaactacg gcttcagctt ctacacgacg
     1201 cggctgatgc acgaggccgg cgtggcgctg gccgaaccgc gcgtctggct cggggatgcc
     1261 gagaagctgc cgctcggcct cgccgagccg ctcggcgtca cggtgcccaa gggcaaggcc
     1321 gagggcctga agtccgagat ccaggtcaat ccgcagatcg aggcgccggt cggccgtggt
     1381 tcggtgcacg gcaaggtcgt cgtcacgctc gatggcaaca cggtcgccga ggcgccgctg
     1441 gtcgcgctgc aggacgtggc cgagggcagc ttcttctcgc ggctgctcga tcacatcctg
     1501 atgctgttcc acaagctgat cggctgaccc ccgcccccgc ctgcgggcgg ggtcacgccc
     1561 gctggtgatt gccatgtccg aagcgccgag cgactcgctg ctcgaatttc cctgcgactt
     1621 cccgatcaag gccttcggcg aaggcgatga tttcgaagcc ctgatcgtcg ggctcgtgcg
     1681 cacgcacgcg cccgatctcg acccggcgcg cgtgcgcgtc gccgcctcgc gcaccggccg
     1741 ctacatcgcc gtgaccgtcg tcgtgccggc gcactcgcgc gcgcagctcg acgcgatcta
     1801 ccgcgacatc agcggccacg cccgcgtgaa gatggcgctg tgaagccgct gcgcagctgg
     1861 tggcggcgcg atctcggccg gcgggcctac gcgccggtct acgcggcgat gaaggcctgg
     1921 accacggcgc gcacggccga tgccggggac gaggtctggg tggtcgaaca cccgccggtg
     1981 ttcacgcaag gcctcaacgg caaggccgag cacctgctcg cgaccggcga catcccggtg
     2041 gtcgcggtcg atcgcggcgg gcaggtcacc tatcacgggc cggggcagat cgtcgtctat
     2101 ccgctgctcg acatcgaacg cgccggcatc ggcgtgcgca cgctggtcga tgcgctcgaa
     2161 ggcgcggtcg tcgacctgct cgccgcgcgc ggcatcgcgg cctgcgcgaa gcccgatgcg
     2221 cccggggtct acgtcgacgg gcgcaagatc gcctcgctcg gcctgcgcgt gcgccgcggg
     2281 cgctgctatc acggactcgc cgtcaacatc gacatggatc tcgcgccgtt tcagcgcatc
     2341 aacccctgcg ggtacgccgg cctgcagatg acacagctgt ccgagctgtg cgatgatttc
     2401 gcgatacaga aagcgacgga cgaattattg catcatctgg cgtcccggct gggcttcgcc
     2461 gatccgctgc cgctttcgga actcccgacc gccttgtccg aggacgcctg agatggccga
     2521 gctgcccgaa ccgacttccg aaacccttgc ccccgccgcg ggcaacgctc ccgtccgccg
     2581 cgtcacggtg accggcgaga agctgcgtgg cgccgacaag gtcgcgcgca tcccggtcaa
     2641 gatcgccccg accacgctgg aaacgcgcct gaagaagccg gaatggatcc gcgcacgctt
     2701 cccgggctcg ccggaggtgc agcgcctcaa gggcatcctg cgcgagaacc gcctgcacac
     2761 ggtctgcgag gaagcctcgt gcccgaacct cggcgaatgc ttcggccacg gcacggcgac
     2821 cttcatgatc atgggcgaca tctgcacgcg ccgctgcccg ttctgcgacg tcggccacgg
     2881 ccggccgaac ccgctcgatc cggacgagcc gctgaacatg gcacgcacga tcgcggcgat
     2941 gggcctgaag tacgtcgtca tcacctcggt cgaccgtgat gacctgcgcg acggcggtgc
     3001 cgcgcatttc gccgaatgca tccggcgcgc gcgcgagctg tcgccggcgc tgcaggtcga
     3061 ggtgctggtg ccggacttcc gcggccgcgt cgagatcgcg gtcgacatcc tcgccgcctg
     3121 cccgcccgac gtgttcaacc acaacctcga aaccgtgccg cggctgtacc gccaggcccg
     3181 gcccggcgcc gactacgaag gctcgctcga tctgctcgaa gcctacaagg cccgccaccc
     3241 gcaggtcgcg accaagtccg gcctgatgct cggcctcggc gaggagctcg acgaggtgcg
     3301 cgcggtgatg cgtgacctgc gcgcgcacgg cgtcgacatg ctgaccctcg gccagtacct
     3361 gcagccgagc ctgcaccacc tgccggtggc gcgctacgtg acgccggccg agttcgagca
     3421 gctgcgcgtc gatggcctgg aaatgggctt cacgcacgtc gcctcgtcgc cgatggtgcg
     3481 ctcctcgtac cacgccgacc tgcaggcgaa gggcgaattc ccgggctgag cggcaaggtc
     3541 cgcacggcag ccaaagacga aggccgcaca ccctcccggg tgtgcggcct tccttttggc
     3601 tttttctagt tgtcgtggaa agcagcccgc gactggcggg ctgcggtttg ccggtgcgct
     3661 tgccggcggt gtcgccgatc agccctgcat cgcgatgcag gccagtcgtg ccttgcgggc
     3721 ccggtagctc gcccagagcc aggggacttc cgtgaacggc accgacaggc ggttgccgac
     3781 gtaacgggcg aattcacgct cggtgagttc gcgccccagt tccagttccg cccggcggac
     3841 gatttcgcgc atctcctcgt tcatggtggg tctcctcctg tccggatctc gcagcgcgga
     3901 agcgattcca cggtgcgcca tcctggggac catatgttgc ggcggtttga ccgctctgtg
     3961 tatgacggaa cgttcatgtc ccgtcagaat gctgcggggg cgtcaccatg acgcccccgc
     4021 gggtctgcag cgcgccgatg cgcccgtcag tcggccggcg cctgggcgat gcgaccggcg
     4081 cgcaggtcga tccagtcgag caggatctgc ccggtctcgt cgaggaaggg atcgggcccg
     4141 tccttcttgg cgtcgccggc atcgtcgctg tccggatcga tgacccggcc gcgcgcggtg
     4201 cgcatgcggc tcttgctggc ttcctgatcg gtgcgacgct tgctctccag cagcgatacg
     4261 gtggtctgct tgcgcagcgc catcgacacc tccaggtcgt gcttcagatc ctggtagggc
     4321 tgatcgtgcg gcacgcgcgc ctcgtgcagg cgcttcagtt cagtgacgcc gtcgcgcacc
     4381 cggccggcca ccttgtagcg ggtcggcggg atttcgtccc agggcagggc gtgctcctgg
     4441 gcgctctcgc cgacctcgtc ggtcgcgaag gccggcggca gcgcgacatc gggcagcacg
     4501 ccgcggttct gcgtgctctc gccgttgatg cggtagaact tggcgatggt cagcttcagc
     4561 tggccgagct tgtccttgac ctgcggcagg aagcggccga gatcgatcag cgtctgcacc
     4621 gtgcccttgc cgaaggtctg ctcgccgacg agcacgccgc ggccgtagtc ctggatcgcc
     4681 gcggcgaaga tttccgaggc cgaggcactg aagcggtcgg tcagcaccgc gagatgcccc
     4741 gaatagaact gcttcggatc gggatcgttc tcgacctgta ccttgccctt ggcatcgcgc
     4801 acctgcacga ccggcccgtc cgcaatgaac agcccggtca gctcgacggc ttcctgcagc
     4861 gagccgccgc cgttctggcg caggtcgatg acgatgccct cgacgcgctc gcgctgcagc
     4921 tgctcgatca gccggcgcac gtcgcgcgtc gtgctgcgat agtcgagatc accgcgctgc
     4981 gcggccgcga agtcgatgta gaaggtcgga atcgtgatga cgccgacctt gcgctcgacc
     5041 ccgtcgcgca cgaaggtctt gacctgactc ttggccgcct gctcctccag ctggatcttg
     5101 ttgcgcacga tgcgcacgac cgtgcccgga ctgccggccg cggcctgcgc cggcaccacc
     5161 tgcaggcgca cgaccgagcc gcgctggccg cggatcaggt cgacgacatc atcgagccgc
     5221 cagccgacca cgtcggtcat cgcgccgtca tcgccctggc cgaccgcgag gatgcggtcc
     5281 ttgggccgga tctgcttgga caggtcggcc ggaccgccgg gcaccagctc gacgaccacg
     5341 gtctgctcgt cctcaagccg cagcaccgcg ccgatgccct ccagcgacag gcgcatctgg
     5401 atgttgaagt tctcggaatt gcgcggactc aggtacgagg tgtgcggatc gaaggcgccg
     5461 gtgacggcgt tcatgtagat ctggaacacg tcctcggggc cggactgcgc ggcgcggcgc
     5521 agccggcctt cgtagcgctt gcgcagcgtg tcgcgggcgg cagcctcgtc cttgccggcg
     5581 agcatcaggc tcagcgtctc gttcttcagg cgcttgcgcc agatctcgtc gagttcggcc
     5641 gccgtgcgcg cccacggtgc cttcttgcgg tcggcctcgt aggtttcgtc cttcctgaag
     5701 tcgaacggcc gcgtcagctc ctcgctgatg cgtctcgtgc gctccagcag ccgctgctga
     5761 tagaggttgt agatcgcgaa ggcagcatcg agccggccgt tgcgcagttc gtcatcgatc
     5821 ttgtcacgat actggccgag ttcggcgaca tcgctggcga gcaggaagct gtggctgaaa
     5881 tcgagcgccg acaggtaatc gtcgaacacc gtcgccgaca gcgcgctgtc gattttgacc
     5941 cgccggtagt ggtactgcgt cagcaggccc gcgaccgtct tgtcgacgct cgactgcacg
     6001 gtatcgggcg acaggtccgg cagggtcttc ggcaggggcg gcagggcgtc gccgacggtg
     6061 gcggcgcagg cgacggtggc aaacgacagc agcagcgcgg ctgccaggct cagtcggcac
     6121 ggggccatgc gggctgctct catgcaacgt ttccgttcgg atgatcgccg tcgtcggcgt
     6181 cctggtcatc gtcgtgcccg gcagagccct cggcctcggc cgccgtactc gccgcggcag
     6241 cggcgctgct gcttgcgccg tgccgggaac tgccgcgcca gccgcgctcg gccatcggcg
     6301 ggcagaccag ctggatgtcg gtggcgaccg gctgcttgcc gctcggctcg gccagcgtga
     6361 actcgaacac atggtggcgc gacggcaggc gcatgacgtt gaagccgtcg ggcaggttgg
     6421 aatcatggaa gaacgccgtg cgatacggcg aatcggggtc gcgcgagtcg gtcttccaga
     6481 gatacggcga gctgcgcacc aggaagcgca tgaagccgaa gctcttgttc tcgtcgtggt
     6541 gatagcagta gccacgcacc cgcgagccga tctcgcccca cggcacgccg gtatcctcgc
     6601 cgcgcgccgg cagcaggccg gggatcagga agcccgagac gtacaggtcg gcctcgttgc
     6661 gcaggtcttc cgagacgttg tcgaagccga tgacctcgac ccggcagccc ttgttctgca
     6721 gcgcccgcac cacctgcacg aaatcgccgt cgccggtcac gagcacgacg cgatcgagat
     6781 tctgcgactg cagcagcagg tcgacggcca tgtcgagatc ggcgttggcc ttgccgtagc
     6841 gggtgccgga ctcgtcctcg aaccagcgga ccttcttttc gatgaccttg tagccgagtt
     6901 cgcgcaacgc cgcctgatac gcgcgcatcc gttcacggta ggtgtagttg ctgttggccc
     6961 gatcctcgtc gaaggtcaga tagacattca ggcgcagcgc ctcggcgtaa tcgcggcagg
     7021 caaattcacg cagcacgtcg tactgcatgc ggctgccgcc gttgcgggaa atattggcgg
     7081 cgtcgacgta aacgccgacg ttggcgtgag acttgatggt cacgaagcta cctcgatgat
     7141 cccgcgccgg tgaggccggc gaaaagcggg atacgacaaa tgatgacaaa gatgaaggcg
     7201 aaaccctgca ggccggacag cccgccgtgg ctgcatgccg gccgatgcgg gccggctgat
     7261 tgtcgcagaa tcccccggga accgcttgtt cgcaggacgc cggatttcgg cgcccttgcc
     7321 tgcacgaccc taggtatctg cacaacttgc agcagcggct cccggacttg tccagacagc
     7381 acggatggat cagcacgacg gcaagacgct gatgagcaag ccc
//
LOCUS       DAOQIE010000199         7377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5257_length_7377_cov_7.283393, whole genome shotgun sequence.
ACCESSION   DAOQIE010000199 DAOQIE010000000
VERSION     DAOQIE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_7377_cov_7.283393"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK144_12430"
     CDS             <1..679
                     /locus_tag="PK144_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNF66578.1"
                     /translation="AAPDGPPVSPVVPDAVLLGLRSIDGARLHERLGDGARAALCELL
                     ARAGDGENELLRLIVDNRRAELQRRAHQLKGVGGALCAHALAEAAAALEKRLRADAEG
                     HEPLPVVETIRLADEYARVLGELREAGYTPAELPPAAAGIDFVHGLARLKALCAATDL
                     EALALARRLGEAQSVGWSAARRRDWAAAVAALERYDFSAAEAGFARFVPSTHDTDRQE
                     EPPHDSS"
     gene            666..4187
                     /locus_tag="PK144_12435"
     CDS             666..4187
                     /locus_tag="PK144_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNF66579.1"
                     /translation="MTRRNRPRAGVPEPGSGVASRASPEADIPAPAEAPADGPPLPGA
                     AAPFLQEILPGSPAEAATETATEAATPAAGREPPGDLYVVGIGASAGGLEALRPLIAN
                     LVPNGRLSYLIAQHMSTQHRSLLVDLLARDAQVLVVEATGGAWLRPDTVYVTPPDRDI
                     LVSSGRIVLRRPGADIGPRPSVDALLNSLATEYAERAIGVILSGTGSDGAHGCRAIKA
                     RGGFTIVQEPGSAKYDAMPRAAQRADAVDLALPPELIARRLPELVDAPAGAVALQSAE
                     EGSGAEGEENGALRGLLERVLRATQTDFTDYKEATLGRQIGRRLAALRLESLSAYLDY
                     VDQRPEELGVLQRSFLISVTEFFRDPDAFRALERALEPLIAGHGGQGPIRVWVPGCAT
                     GEEAYSLAMLFAQRLGSRAVGSVKIFATDIDPSATEFARRGIYPEPAVQGLEPELRER
                     WFRQEGRHYLISKTIRDLCVFARQDLVRDPPFLRMDLISCRNLLIYLKPNLQERLLNQ
                     FHYSLTPGGYLFLGKSENITQSGRLFSTVDGRYKLFRRKSVLTPRPLMIGSGNLGIGQ
                     PASTGNRSVPSRQARLEAMRDELLDRYAPPSILLGPGLEPMHFCGDARRFLRLPDGPA
                     DFGLLSLLLPEMRGEFRTLQHRLQSADGSDHVSGHMVPVHTTLGSEYVRMRLSRLTVP
                     EASEDMMLLSIEPMRATEPPPAAVSTGAAPAGSDHDHAVLTERLRDAENELGATREHL
                     QAVIEELETSNEELQSLNEELQASTEELQSSNEELETTNEELQATNEELTTVNDELEA
                     KSEELTQVNTQLVNIQQSVALGLIVVDADLRVMRYNPPAVRVFGLMNDDLGQSLLAVP
                     SFVEIGDLRRKLLDVVGSGRTLSEELRREEATFLMQIAPYREDSGVISGAVITLSDIT
                     RFRELERAHAEGDQRLRLIADALQDAIWLATPGFTRMLYVSRGYFSLWERTPESLYAD
                     PHSFLDAVHFEDRARVRRQLLETDPPATDLTYRLLVPGAGQRWVRQCTSLVRCEESGP
                     ALYLATSVRDVTAAAAERERLQHSEMNLRTAIDLLPIAALLRDAEGRVVVASGALSAL
                     LGYRPEALVGHPLGDVLCAEDAEGERARYLAARAAGERSYTQAVDWRRADGTTVPGRL
                     SVRFAASSDEVGDLSLALALLDELPFG"
     gene            4220..4744
                     /locus_tag="PK144_12440"
     CDS             4220..4744
                     /locus_tag="PK144_12440"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNF66580.1"
                     /translation="MPDPAAVRLCGFPPLVGERPRVLVLGSMPGAASLAALEYYGHPR
                     NAFWPIMGELFGAGPALPYAERTARLGAAGVALWDVLAACRRPGSLDSAIEPDSIELN
                     DIPGLLAAQPGIRHVFCNGTAAATLLRRHVLGRLPRALPWTRLPSTSPAHAGRSLADK
                     LAAWRVLADTLAVD"
     gene            complement(4741..5550)
                     /locus_tag="PK144_12445"
     CDS             complement(4741..5550)
                     /locus_tag="PK144_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNF66581.1"
                     /translation="MTACVHVAALQALMLDDPAGKCARAASLYADWQAGALACDAQAP
                     TAWVDLPGRPPRPLLVPARAVPRRPPGTPAGRVALVHAVAHIEFNAINLALDAVQRFR
                     GLPDDFYGDWLRVAAEEARHFELLTARLQAMDSAYGELPAHDGLWDAARRTAHDPLVR
                     MALVPRVLEARGLDVTPGMIERLRQAGDAETVAVLEVILAEEVGHVAIGSRWYAHLCA
                     ERGLEPRSTFRRLLAEHRTAVFPPYNVEARLAGGFAAEELDALAADAAAGR"
     gene            5759..7114
                     /locus_tag="PK144_12450"
     CDS             5759..7114
                     /locus_tag="PK144_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66582.1"
                     /translation="MPAIPPLRALSPHPRLRRTALGLAFATLLPLAPVQAAPIALLSA
                     SLDTAFSLDGFSTGAANQFTLSLAAASGSLAFNRPADGLYGVTANGNVTLASDAAYSD
                     VLLNRSYVNQNLFFGQIATGDVGSQNVSFDFTNGLYTRDGVTTANGASGSFTTSYNGF
                     ANFISGLAGGPALAPGSGSGTLSVSYTIDAAKNASFTITDTPVTENGWQGFEQYIRDW
                     QANRGNSGDHVDGYVRNTSAFTLTATAAQAGDGSSPLTPLLPTSTDPQTVTVQTPDGP
                     VQTTTSEYSFAPQSITPGQRVSYDPFYASGYVYEISNAAGPKLAALFLPVVGDNEYLL
                     EFSSDGGTSWSLASWVGSNGTVFGNVLYSLLNATLAPTGLSMLRLTGIETSAMIDPND
                     PLGFVTTFGYAQAGTQGLTMTAIPDFVADATAIPAPAPLALIGLGLTALALGRRRLKV
                     A"
     gene            complement(7187..>7377)
                     /locus_tag="PK144_12455"
     CDS             complement(7187..>7377)
                     /locus_tag="PK144_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66583.1"
                     /translation="AQEQTWEEHEHGGRITHARHSHITRHHPARSCTPAGRMRIMRTQ
                     CRVSNIWAEAWAGRVQLT"
BASE COUNT         1057 a         2762 c         2545 g         1013 t
ORIGIN      
        1 ggcggccccg gacggcccgc ccgtgtcgcc cgtggtgccg gacgcagtgc tgctcgggct
       61 gcgcagcatc gacggcgcgc ggctgcacga gcgcctcggc gacggcgcgc gggccgcgct
      121 ctgcgaactg ctggcccgcg ccggcgacgg cgagaacgag ctgctgcgcc tgatcgtcga
      181 caaccggcgc gccgagctgc agcgccgtgc gcatcagctc aagggcgtcg gcggcgcgct
      241 gtgcgcacac gcgctcgccg aggccgccgc cgccctcgaa aagcggctgc gcgccgatgc
      301 cgagggccac gaaccgctgc cggtcgtcga gaccatccgg ctcgccgatg aatatgcgcg
      361 ggtgctggga gaactgcgcg aggccggcta cacccccgcc gagctgccgc cggcggcggc
      421 cggcatcgat ttcgtccacg gactcgcacg cctgaaggcg ctctgcgcgg ccaccgatct
      481 cgaagcgctg gcgctggcgc gccggctcgg cgaggcgcag tctgtcggat ggtcggccgc
      541 ccggcgccgg gactgggcgg cggccgtcgc cgcgctcgag cgctacgatt tctccgctgc
      601 ggaagccggt ttcgcccgct tcgttccttc aacgcacgac acggaccgtc aggaagagcc
      661 tccccatgac tcgtcgtaac cgcccacgcg ccggcgtgcc ggagccaggc tccggcgtcg
      721 cctcgcgcgc ctcccccgaa gcggacattc cggcaccggc cgaagcgccg gcggacgggc
      781 cgccgctgcc cggggccgct gctccgttcc tgcaggagat tcttccgggc agtccggccg
      841 aggccgcgac cgagaccgca accgaggccg cgacccccgc ggccgggcgc gagccgccgg
      901 gcgatctgta cgtggtcggc atcggggcct cggccggcgg tctcgaagcc ctgcgcccgc
      961 tgatcgccaa cctcgtgccg aacggccggc tgagctacct gatcgcgcag cacatgtcga
     1021 cgcagcaccg cagcctgctg gtcgacctgc tggcgcgcga tgcgcaggtg ctcgtggtcg
     1081 aggcgaccgg cggtgcctgg ctgcgtccgg acacggtcta cgtgacaccg ccggatcgcg
     1141 acatcctggt ttcgagcggc cgcatcgtgc tgcgccgccc cggcgccgac atcggcccgc
     1201 ggccctcggt cgatgcgctg ctgaactcgc tcgcgaccga atacgccgag cgcgcgatcg
     1261 gcgtgatcct ctccggcacc ggctccgacg gcgcgcacgg ctgccgcgcg atcaaggcgc
     1321 gcggcggctt cacgatcgtg caggagccgg gcagcgccaa gtacgacgcg atgccgcgtg
     1381 ccgcccagcg cgccgatgcc gtcgatctgg cgctgccgcc cgagctgatc gcccggcgcc
     1441 tgcccgagct ggtcgacgcg ccggccggag cggtggcgct gcaatcggcc gaggaaggca
     1501 gcggggccga gggcgaggaa aacggcgcgc tgcgcggcct gctcgaacgc gtgctgcgcg
     1561 ccacgcagac cgacttcacc gattacaagg aagcgacgct cgggcgccag atcggccggc
     1621 ggctcgcggc gctgcgtctc gaatccctga gcgcctatct cgattacgtc gaccagcgcc
     1681 ccgaggaact cggcgtgctg cagcgcagct tcctgatctc ggtgaccgag ttcttccgcg
     1741 atccggatgc cttccgcgcg ctcgaacgcg cgctcgaacc gctgatcgcc gggcacggcg
     1801 gccaggggcc gatccgcgtc tgggtgccgg gctgcgcgac cggcgaggaa gcctattcgc
     1861 tggcgatgct gttcgcgcag cgcctcggca gccgcgccgt cggcagcgtc aagatcttcg
     1921 cgaccgacat cgacccgtcg gcgaccgagt tcgcgcgccg cggcatctat ccggagccgg
     1981 ccgtgcaggg gctcgaaccg gagctgcgcg agcgctggtt ccgccaggaa ggccggcact
     2041 acctgatcag caagacgatc cgcgacctgt gcgtgttcgc ccgccaggat ctggtccgcg
     2101 acccgccgtt tctgcgcatg gacctgatca gctgccgcaa cctgctgatc tacctgaagc
     2161 cgaacctgca ggagcggctg ctgaaccagt tccactactc gctgacgccg gggggctacc
     2221 tgttcctcgg caagtccgag aacatcacgc agagcggccg gctgttctcg accgtcgacg
     2281 gccgctacaa gctgttccgg cgcaagtccg tgctgacgcc gcgcccgctg atgatcggca
     2341 gcggcaacct cggcatcggc cagccggcca gcaccggcaa ccgcagcgtg ccctcgcgcc
     2401 aggcgcggct ggaggcgatg cgcgacgagc tgctcgatcg ctacgcgccg ccgagcatcc
     2461 tgctcggccc cgggctggag ccgatgcact tctgcggcga cgcccggcgc ttcctgcgcc
     2521 tgccggacgg cccggccgac tttggcctgc tgtcgctgct gctgccggag atgcgcggcg
     2581 agttccgcac gctgcagcac cggctgcagt ccgccgacgg cagcgaccac gtcagcggcc
     2641 acatggtgcc ggtgcacacg acgctcggct ccgagtacgt gcgcatgcgc ctgagccggc
     2701 tgaccgtgcc cgaggccagc gaggacatga tgctgctcag catcgagccg atgcgcgcga
     2761 ccgagccgcc gccggcggcc gtcagcaccg gggccgcgcc ggccggcagc gatcacgacc
     2821 acgcggtgct gaccgagcgc ctgcgcgacg ccgagaacga actcggcgcg acgcgcgagc
     2881 acctgcaggc ggtcatcgag gaactcgaga cctcgaacga ggagctgcag tcgctgaacg
     2941 aggaactgca ggcctcgacc gaggaactgc agagctcgaa cgaggaactc gaaaccacca
     3001 acgaggagct gcaggccacc aacgaggagc tgaccaccgt caacgacgag ctggaggcga
     3061 aatccgagga gctgacgcag gtcaacaccc agctcgtcaa catccagcag tcggtggcgc
     3121 tcggcctgat cgtcgtcgac gccgacctgc gcgtgatgcg ctacaacccg ccggccgtgc
     3181 gcgtgttcgg cctgatgaac gacgacctcg gccagagcct gctggccgtg ccgtccttcg
     3241 tcgagatcgg cgacctgcgc cgcaagctgc tcgacgtggt cggcagcggc cgcacgctgt
     3301 ccgaggagct gcgccgcgag gaagccacct tcctgatgca gatcgcgccg tatcgcgagg
     3361 acagcggcgt gatctccggc gccgtgatca cgctgtcgga catcacgcgc tttcgtgagc
     3421 tggagcgcgc gcacgccgag ggcgaccagc gcctgcgcct gatcgccgac gcgctgcagg
     3481 acgccatctg gctggcgacc ccgggcttca cgcgcatgct ctacgtcagc cgcggctact
     3541 tctcgctgtg ggaacgcacg cccgaatcgc tgtacgccga tccgcattcc ttcctcgatg
     3601 ccgtgcattt cgaggatcgc gcgcgcgtgc gccgccagct gctggaaacc gatccgccgg
     3661 ccaccgacct gacctaccgg ctgctggtgc ccggcgccgg ccagcgctgg gtgcgccagt
     3721 gcacgtcgct ggtgcgctgc gaagaaagcg gcccggcgct gtatctggcc accagcgtgc
     3781 gcgacgtgac cgcggccgcc gccgagcgcg agcggctgca gcacagcgaa atgaacctgc
     3841 gcacggccat cgacctgctg ccgatcgccg cgctgctgcg cgacgccgag ggccgcgtgg
     3901 tggtcgcaag cggcgcgctg tcggcgctgc tcggctaccg gccggaggcg ctggtcggcc
     3961 atccgctcgg cgacgtgctg tgcgccgagg acgccgaggg cgagcgcgcg cgctatctgg
     4021 ccgcgcgtgc cgccggcgag cgcagctaca cgcaggccgt ggactggcgg cgcgccgacg
     4081 gcacgacggt gccggggcgc ctgagcgtgc gcttcgcggc ctccagcgac gaggtcggtg
     4141 atctgagcct ggcgctggcg ctgctcgacg agctgccgtt cggctgatgc ggtgcggagg
     4201 cgatgacgat ccggcgctga tgccggaccc ggccgcggtg cggctgtgcg gcttcccgcc
     4261 actcgtcggc gagcggccgc gcgtgctggt gctcggctcg atgccgggcg cggcctcgct
     4321 cgccgccctc gagtactacg gccatccgcg caacgccttc tggccgatca tgggcgagct
     4381 gttcggcgcc ggtccggcgc tgccctatgc cgagcgcacg gcgcggctgg gcgccgccgg
     4441 cgtggcgctc tgggacgtgc tcgccgcctg ccgccgcccc ggcagcctcg acagcgcgat
     4501 cgagccggat tcgatcgaac tcaacgacat tccgggcctg ctcgccgcgc agcccggcat
     4561 ccgtcacgtg ttctgcaacg gcacggccgc cgcgacgctg ttgcgccggc acgtgctcgg
     4621 ccgcctgccg cgggcgctgc cgtggacgcg gctgccatcg accagtccgg cgcacgccgg
     4681 gcgcagcctg gccgacaagc tcgccgcgtg gcgggtgctg gccgacaccc tggccgtgga
     4741 ctagcgcccg gccgcggcat cggccgcgag cgcgtcgagt tcctccgctg cgaagccgcc
     4801 ggccagccgc gcctcgacgt tgtacggcgg gaacaccgcg gtgcggtgct cggcgagcag
     4861 ccggcggaag gtcgagcgcg gctccagccc gcgctcggcg caaagatgcg cgtaccagcg
     4921 cgaaccaatg gcgacgtggc cgacctcctc ggcgaggatg acttcgagca cggcgacggt
     4981 ttcggcatcg ccggcctgac gcaggcgttc gatcatgccc ggcgtcacgt cgaggccgcg
     5041 cgcctccagc acgcgcggca ccagcgccat gcgcaccagc ggatcgtgtg cggtgcgccg
     5101 cgcggcatcc cagagcccgt cgtgcgccgg cagctcgccg taggccgaat ccatggcctg
     5161 cagccgggcc gtcagcagtt cgaaatgccg ggcttcctcg gcggcgacgc gcagccagtc
     5221 gccgtagaag tcatcgggca ggccgcggaa gcgctgcacg gcatcgagcg cgaggttgat
     5281 ggcgttgaac tcgatgtgcg cgaccgcgtg caccagcgcc acgcgaccgg ccggcgtgcc
     5341 gggcgggcgc cgtggcacgg cgcgtgccgg caccagcagc ggccggggcg ggcggccggg
     5401 caggtcgacc caggccgtcg gtgcctgcgc gtcgcaggcg agcgcacccg cctgccagtc
     5461 ggcgtacagc gaagccgccc gcgcgcactt gccggcgggg tcatcaagca tcagcgcctg
     5521 cagggcagcg acatggacgc aggccgtcat cggaaaatcc tcgcatcggt gcaggaccgg
     5581 gccgcgcagt ctaccgtctg tgccctgccg attcagccac ttgcggctgt tctggcagga
     5641 accccgacga cgaccggtgg tcaggtcctg cgcgaacggt cctgccggcc gcccgctacc
     5701 ggttccggcc cgccgtggcc gtgacactcc gctgcttccg tccaggagga cgcttccgat
     5761 gcccgcaatc ccccctctgc gtgccctgag tccacacccc cgcctgcgcc gcaccgctct
     5821 cggcctggcg ttcgccacgc tgctgccgct cgcgcccgtg caggccgcgc cgatcgcgct
     5881 gctgtcggca agcctcgaca ccgccttcag cctcgatggt ttcagcaccg gcgccgccaa
     5941 ccagttcacg ctgtcgctgg ccgcggccag cggctcgctg gccttcaacc gcccggccga
     6001 cggcctctac ggcgtcaccg ccaacggcaa cgtcacgctg gcgagcgatg ccgcctacag
     6061 cgacgtgctg ctgaaccgga gctacgtcaa ccagaacctg ttcttcggcc agatcgccac
     6121 cggcgatgtc ggctcgcaga acgtcagctt cgacttcacg aacggtctct acacgcgcga
     6181 cggcgtcacc actgccaatg gcgccagcgg cagcttcacg accagctaca acggcttcgc
     6241 gaacttcatc agcggcctgg ccggcggccc ggcgctcgcg cccggcagcg gctccggcac
     6301 gctgagcgtc agctacacga tcgacgccgc gaagaacgcg agcttcacga tcaccgacac
     6361 cccggtcacc gagaacggct ggcagggctt cgagcagtac atccgcgact ggcaggccaa
     6421 ccgcggcaac agcggcgatc acgtcgatgg ctacgtgcgc aacaccagcg ccttcacgct
     6481 gaccgccacc gccgcgcagg ccggcgacgg ctccagcccg ctgacaccgc tgctgccgac
     6541 ctcgaccgac ccgcagaccg tcaccgtgca gacacccgac ggcccggtgc agacgacgac
     6601 cagcgaatac agcttcgcgc cgcagagcat caccccgggc cagcgcgtga gctacgaccc
     6661 gttctacgcc agcggctacg tctacgagat cagcaacgcc gccggtccga agctcgcggc
     6721 gctgttcctg ccggtggtcg gcgacaacga gtacctgctc gaattctcca gcgacggcgg
     6781 cacgagctgg agcctcgcaa gctgggtcgg cagcaacggc acggtgttcg gcaacgtgct
     6841 ctacagcctg ctgaacgcca cgctcgcgcc gaccggcctg tcgatgctgc gcctgaccgg
     6901 catcgagacc tcggcgatga tcgacccgaa cgacccgctc ggcttcgtca ccaccttcgg
     6961 ctacgcgcag gccggcaccc agggcctgac catgaccgcg atcccggact tcgtcgccga
     7021 cgccacggcc atcccggccc cggcaccgct ggcgctgatc ggcctcggcc tgacggcgct
     7081 cgcgctcggc cgccggcgtc tgaaggtcgc ctgaagcttg caggcaacgg gcgtccgcac
     7141 tgccgcaagg gcagggcggg cgcccgcttt tttttggtgc gtgaattcac gtcagctgaa
     7201 cacgcccggc ccatgcctcc gcccagatat ttgaaacgcg gcactgcgtg cgcatgatgc
     7261 gcatcctgcc cgccggcgtg cacgaccgcg ccggatgatg ccgggtgata tgagaatgtc
     7321 ttgcatgagt gattcggccg ccatgttcgt gctcctccca ggtttgttcc tgtgctt
//
LOCUS       DAOQIE010000200         7304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5344_length_7304_cov_5.303766, whole genome shotgun sequence.
ACCESSION   DAOQIE010000200 DAOQIE010000000
VERSION     DAOQIE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5344_length_7304_cov_5.303766"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK144_12460"
     CDS             complement(<1..405)
                     /locus_tag="PK144_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNF66584.1"
                     /translation="MAADESLLAAATPRLRILMLVHGFNSLSQRLHVELAARGHVLSV
                     EYDIHDSITLEAVELFRPDVVLAPYLKRAIPEAIWRNLPCLVVHPGIPGDRGPAALDR
                     AILERRSHWGVSVLQAVAEFDAGPIWAHRSFPM"
     gene            complement(405..1403)
                     /gene="hypE"
                     /locus_tag="PK144_12465"
     CDS             complement(405..1403)
                     /gene="hypE"
                     /locus_tag="PK144_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNF66585.1"
                     /translation="MQHGGGGRAMAQLVEELFVAAFANPALSALNDQAVLDAGLSGRL
                     VIATDSHVVSPLFFPGGDIGSLAVHGTVNDVAMAAAEPRWLAASFILEEGFALAELAR
                     IVDSMAQAARAAGVAIVTGDTKVVEAGKGDGVFITTTGVGVARAGFETGGTRARPGDV
                     VLVSGAIGDHGIAIMACREGLDFDVPIESDSAALHELVGALCAVLPPPALRVLRDPTR
                     GGLGATLNEIARASGVGMQLAETAIPVHEPVRAACEFLGLDPLYVANEGKLIAIVDPD
                     HAEAALAALRAHPLGREAARIGVCTDDGQRFIELETGFGGRRVIDWIAGEQLPRIC"
     gene            complement(1525..1884)
                     /locus_tag="PK144_12470"
     CDS             complement(1525..1884)
                     /locus_tag="PK144_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66586.1"
                     /translation="MNLTASALLGLLEEAGTDVLTLAEGLSEDELLNSRITRTEIRRL
                     LISMAQTAASLPAGLRTRVPEIDWDGWAALAVALNGSEAEVCDGLWHGVRSQVPATLM
                     WLRVYRQNQPELFAFVP"
     gene            complement(1929..3062)
                     /gene="hypD"
                     /locus_tag="PK144_12475"
     CDS             complement(1929..3062)
                     /gene="hypD"
                     /locus_tag="PK144_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNF66587.1"
                     /translation="MKYVDEFRDGTLARRLAAAIAREVDPAREYRFMEFCGGHTHAIA
                     RYGVEDLLPANVRLIHGPGCPVCVLPTGRLEMAIALAERPGVMLASYGDMLRVPGSGG
                     DSLLKARARGADVRMVYSIDDALALARRHPDRQVIFFAIGFETTTPPTAVAIRQAAAE
                     GLGNFSVFCNHVLTPSAIASILESPEVRDLGSVKLDGFVGPAHVSTVIGSRPYEYFAA
                     EYAKPVVIAGFEPLDVLQAVRMLVRQVNAGQSCVENEFARAVSRDGNRKAQKLVAEVF
                     ELRKHFDWRGLGRVPYSALRIKSAWADFDAERRFAPEPRTVPDHKACECAAILRGVKR
                     PQDCRLFGSACTPETPMGSCMVSSEGACAAHWSYGRLRSARPA"
     gene            complement(3059..3337)
                     /locus_tag="PK144_12480"
     CDS             complement(3059..3337)
                     /locus_tag="PK144_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNF66588.1"
                     /translation="MCLAIPARIVETLPDDEALVDLGGVRKRISLALVEDCAVGDYVI
                     VHVGYALNRLDPDEAARTLALFAEAGLLGDETGDDAGDGAGRDAEVRS"
     assembly_gap    3383..3482
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3521..3937
                     /locus_tag="PK144_12485"
     CDS             3521..3937
                     /locus_tag="PK144_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNF66589.1"
                     /translation="MEILYPAILEPQSGGGFCVRFVDFDEAFTEGETVEEAQFNAIDV
                     LTLTLEGRLEEGLPIPEPTPDLSGAVYIAPDARTQSALLIRRARGERSLADIARVLQT
                     TWPAAERLEDPRHWPSLRQLDRAAAALGKRLVLRFE"
     gene            complement(3982..4371)
                     /locus_tag="PK144_12490"
     CDS             complement(3982..4371)
                     /locus_tag="PK144_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66590.1"
                     /translation="MSKHAQHHLDAFSRRWLDLWTAVPVVVAIRVGRMMTAGPSLSAR
                     DHREFTRMGSEKFDALGESWLAMVLQWQNECLRLAMTPWWLAPDALQQAGNEFGRSLA
                     KVAHQGLEPIERRASANARRLVRLRPR"
     gene            complement(4569..6875)
                     /gene="hypF"
                     /locus_tag="PK144_12495"
     CDS             complement(4569..6875)
                     /gene="hypF"
                     /locus_tag="PK144_12495"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNF66591.1"
                     /translation="MNIPVPAPTARLALQVRGLVQGVGLRPWIWRLAHEHGLAGWVRN
                     DGDGVAIEVQGPAPALALFRAALAVPPPLARLDAIEAQTLAPLAGSDGFAILDSGRGP
                     VRTGIGADTAVCADCLAETFDPAGRRWRYPFTTCTHCGPRYTIAARLPWDRPNTALAG
                     FALCASCREEYENPADRRFHAEALACPACGPRLRLQRPDGSRAEVEGDPLAATLALFR
                     AGGIVAVKGLGGFHLMCDARNAAAVAGLRRRKRRPGKPFALLAANPASLAGLVSTDAP
                     ARALLESPQRPIVLLPAPAADAHLPGIAPGLDRLGVMLPATPLQYLLFHEAAGRPAGS
                     DWLRAAQSALFVCTSANPSGEPLAIDDAEACTRLAGIADAVLGHDRAILQRVDDSVLL
                     ARTGKPLFLRRARGWTPQEIRLPAAGPPVLALGGELKNTVCVTRADRAWLSQHLGDLG
                     SAAARRALADTVAHLLAVLDIRPERVACDLHPDSAAAHFASEYAARHDLPLIPVQHHH
                     AHLAAVLAEHGHTGAVLGLVLDGFGLGHDGTLWGGEWLSKEGQRLGRLAPMRLPGGDA
                     AAREPWRMAASALHALGRAADIPARLPQPATRTVAAMLAKGLHCPPTSSCGRLFDAAA
                     GLLGVCAVNTYEGEAAIRLEAAARRHGPVEALAGRWRSFEDGTLDLLPLLASLADETG
                     PGRGAARFHATLAAALAAHSLRLAARHRLDTVALAGGCCANLLLVGDLRARLEAAGLR
                     VLEACELPPNDGGLALGQAWVAMGGDDA"
     gene            complement(6888..>7304)
                     /gene="hypB"
                     /locus_tag="PK144_12500"
     CDS             complement(6888..>7304)
                     /gene="hypB"
                     /locus_tag="PK144_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNF66592.1"
                     /translation="PAVQINTGKGCHLDADMIARALPRLPLDPGTLLFIENVGNLVCP
                     AAFDLGEAAKVAILSVTEGEDKPLKYPDIFAAASLVLLNKSDLLPHLDFDLEYALDCL
                     RRVNPRAPVLSVSAKTGEGLDAWLAWLRTPAPVAAG"
BASE COUNT         1054 a         2507 c         2691 g          952 t
ORIGIN      
        1 catcgggaag ctgcggtgtg cccagatcgg gccggcatcg aactcggcga ccgcctgcag
       61 cacgctgacg ccccagtgcg aacggcgttc aaggatggcg cgatcgagtg ccgccgggcc
      121 gcggtcgccg gggatgcccg gatgcacgac cagacagggc aggttgcgcc agatcgcctc
      181 ggggatcgcg cgcttcaggt acggtgcgag cacgacatcg gggcggaaca gctcgacggc
      241 ttcgagcgtg atcgaatcgt ggatgtcgta ctcgaccgac agcacgtggc cgcgggcggc
      301 gagttcgacg tgcaggcgct ggctcagcga attgaagccg tgcacgagca tcaggatgcg
      361 cagccgcggt gtcgccgccg ccagcaggga ctcgtcggcg gccatcagca gatcctcggc
      421 agctgttcgc cggcgatcca gtcgatgacg cgccggccgc cgaagccggt ttcgagttcg
      481 atgaagcgct ggccgtcatc ggtgcagacg ccgatgcgcg cggcctcgcg cccgagcgga
      541 tgcgcgcgca gcgcggcgag ggcggcttcg gcgtgatccg gatcgacgat ggcgatcagc
      601 ttgccttcgt tggcgacgta cagcgggtcg aggccgagga actcgcaggc ggcgcgcacc
      661 ggctcatgca ccgggatcgc ggtctcggcg agctgcatgc cgacgccgga agcgcgcgcg
      721 atctcgttca gcgtcgcacc gaggccgccg cgggtcgggt cgcgcagcac gcgcagcgcc
      781 ggcggcggca gcaccgcgca gagcgcgccg accagttcgt gcagcgcggc gctgtccgat
      841 tcgatcggca cgtcgaaatc caggccctcg cggcaggcca tgattgcgat gccgtgatcg
      901 ccgatcgcgc cgctcaccag caccacgtcg ccgggccgtg cgcgcgtgcc gccggtctcg
      961 aagcccgcgc gcgcgacgcc gacgccggtc gtggtgatga acacgccatc gcccttgccg
     1021 gcctcgacga ccttggtgtc gccggtgacg atggccacac cggcggcgcg tgcggcctgc
     1081 gccatcgaat cgacgatgcg ggcaagctcc gcaagcgcaa agccttcctc caggatgaag
     1141 ctcgccgcca gccagcgcgg ctcggccgcc gccatcgcca cgtcgttgac cgtgccgtgc
     1201 acggcgaggc tgccgatgtc gccgccgggg aagaacagcg gcgagacgac gtggctgtcg
     1261 gtggcgatca ccagccgccc ggaaagcccg gcatcgagca cggcctgatc gttcagcgcc
     1321 gacagcgcgg ggtttgcgaa cgcggcgacg aacagctcct cgacgagctg cgccatcgcg
     1381 cggccgccac cgccgtgctg catctcgatg cagccgtggc gcaggtccag tttgcgggga
     1441 aagtctctcg gggggctcaa cggggcagac ctgtcgggat cggggggaaa tcggggcagg
     1501 gaagcgcttc gtgcggcccg gggctcaggg cacgaacgcg aacagctcgg gctggttctg
     1561 gcggtagacg cgcagccaca tcagcgtcgc cggcacctgc gagcgcacgc cgtgccagag
     1621 tccgtcgcag acttcggcct cgctgccgtt cagcgccacg gccagcgctg cccagccgtc
     1681 ccagtcgatc tcgggcacgc gcgtgcgcag cccggccggc aggctcgcgg cggtctgcgc
     1741 catgctgatc agcagccggc ggatctcggt gcgcgtgatg cgcgagttca gcagttcgtc
     1801 ctcgctgagg ccttcggcga gcgtcagcac gtcggtgccg gcttcttcga ggaggccgag
     1861 cagggcggaa gcggtcagat tcatgcgggc ggctccggag gcaggcgggc aggaggcgga
     1921 tacgggtttc aggccgggcg agcgctgcgc aagcgcccgt agctccagtg cgcggcgcag
     1981 gcgccttcgc tggagaccat gcacgagccc atcggcgttt ccggcgtgca ggcgctgccg
     2041 aacagccggc agtcctgcgg gcgcttgacg ccgcgcagga tcgccgcgca ttcgcaggcc
     2101 ttgtgatcgg gcacggtgcg cggttcgggc gcgaagcggc gttcggcgtc gaagtccgcc
     2161 caggcggact tgatgcgcag cgcgctgtac ggcacgcggc cgagcccgcg ccagtcgaag
     2221 tgcttgcgca gctcgaacac ctcggcgacc agcttctgcg ccttgcggtt gccgtcgcgg
     2281 ctgaccgcgc gcgcgaattc gttctcgacg cagctctgtc cggcgttgac ctggcgcacg
     2341 agcatgcgca cggcctgcag cacgtcgagc ggctcgaagc cggcgatgac gaccggcttc
     2401 gcgtattcgg cggcgaagta ttcgtacggg cggctgccga tcacggtcga gacgtgcgcc
     2461 gggccgacga agccgtcgag cttgacgctg ccgagatcgc gcacctcggg cgattcgagg
     2521 atgctggcga tcgccgaggg cgtcagcacg tggttgcaga acacgctgaa gtttccgagc
     2581 ccttcggccg cggcctggcg gatcgcgacc gcggtcggcg gcgtcgtggt ctcgaagccg
     2641 atcgcgaaga agatcacctg gcggtcggga tgcctgcgtg cgagcgccag cgcgtcgtcg
     2701 atcgaataga ccatgcgcac gtcggcaccg cgcgcgcgcg ccttcagcag gctgtcgccg
     2761 cccgagccgg gcacgcgcag catgtcgccg tagctcgcga gcatcacgcc cgggcgttcg
     2821 gcgagtgcga tcgccatctc cagccggccg gtcggcagca cgcataccgg gcagccgggg
     2881 ccgtggatca gccggacgtt ggccggcagc agatcctcga cgccatagcg ggcgatcgcg
     2941 tgcgtgtggc cgccgcagaa ctccatgaag cggtactcgc gcgccggatc gacctcgcgg
     3001 gcgatcgccg cggccagccg gcgggccagc gtgccgtcac ggaactcatc gacgtacttc
     3061 acgaacgcac ctccgcatcc cggccggccc cgtcaccggc gtcatcaccg gtctcgtcac
     3121 cgagcagccc ggcctcggcg aacagcgcca gcgtgcgcgc cgcctcgtcc ggatcgagcc
     3181 ggttcagcgc gtagccgacg tgcacgatca cgtaatcccc gaccgcacag tcctcgacca
     3241 gcgccagcga gatgcgcttg cgcacgccgc cgagatcgac gagtgcctcg tcatccggca
     3301 gggtttcgac gatgcgggcg ggaatggcga ggcacatggc gagcgttccg tcagggagga
     3361 caggggatcg ggcggctgca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nngccagacc ggcgtccggc tcaagcgcta gggcttcagc atggaaattc tgtatccggc
     3541 cattctcgaa ccgcagtctg gaggcggttt ctgcgtgcgt ttcgtcgatt tcgacgaagc
     3601 cttcaccgaa ggtgaaaccg tcgaggaagc gcagttcaac gcgattgatg tgctgacgct
     3661 gacgctggaa ggtcgtctgg aagaagggct gccgattccc gagccgaccc ccgatctttc
     3721 cggggccgtc tacatcgccc ccgatgcccg cacgcagtcg gcgctgctga tccgccgggc
     3781 acggggggag cgttcgctgg ccgacatcgc gcgcgtgctg cagaccacct ggccggccgc
     3841 cgagcggctc gaagacccgc gccactggcc gagtctgcgt cagctcgatc gagccgccgc
     3901 ggcgctcggc aagcggctgg tgctgcgttt cgaatagccc gggcggctcg ggcagcccgg
     3961 gcagcgaagg cgcgcggcgg ctcagcgcgg gcgcagtcgc accaggcgcc gggcgttggc
     4021 cgaggcgcgg cgctcgatcg gctccagccc ctgatgggcg accttggcca gcgagcgccc
     4081 gaactcgttg cccgcctgct gcagcgcatc gggcgccagc caccacggcg tcatcgccag
     4141 tcgcaggcat tcgttctgcc actgcaggac catcgccagc cagctctcgc cgagggcatc
     4201 gaacttctcc gagcccatgc gcgtgaattc gcgatgatcg cgcgccgaca gcgacgggcc
     4261 ggcggtcatc atgcggccga cgcggatcgc cacgacgacc ggtacggccg tccacagatc
     4321 gagccagcgg cgggaaaagg catcgaggtg gtgctgggca tgtttcgaca tgacgggggg
     4381 actccggctt tcggggggga ttcggatcaa tccggctggc cggatgagca atgcgggcct
     4441 cgcgcgacgg gctgacagaa ctgaccgcag ccgccccccg gcggttcgga cgcaggccgg
     4501 aatacttcgg cacgccctgc gcgcgcgcgg cccccgcgcg gcccggaacg ccagcggacg
     4561 agcggccttc aggcatcgtc gccccccatc gccacccagg cctgcccgag cgccagcccg
     4621 ccgtcgttcg gcggcagctc gcaggcttcg agcacgcgca ggccggcggc ttcgagccgc
     4681 gcgcgcaggt cgccgaccag cagcaggttc gcgcagcagc cgccggcgag cgcgacggta
     4741 tcgagccggt gccgggcggc cagccgcagg ctgtgagcgg ccagcgccgc agcgagcgtc
     4801 gcgtggaagc gggcggcgcc gcgtccgggc ccggtttcgt cggcgaggct cgcgagcagc
     4861 ggcagcaggt cgagtgtgcc gtcctcgaaa gatcgccagc gcccggcgag cgcttcgacc
     4921 ggcccgtggc ggcgggcggc ggcttcgagg cggatcgcgg cctcgccttc ataggtgttg
     4981 acggcacaga cgccgagcag gccggcggcg gcgtcgaaca gccggccgca gctgctggtc
     5041 ggcgggcagt gcaggccctt ggccagcatc gccgcgaccg tgcgggtggc cggctgcggc
     5101 aggcgggccg ggatgtcggc ggcgcgaccg agcgcatgca gcgccgaggc cgccatgcgc
     5161 cagggctcgc gtgccgcggc atcgccgccg ggcagccgca tcggcgcgag ccggccgagg
     5221 cgctggcctt ctttcgaaag ccactcgccg ccccacagcg tgccgtcgtg accgaggccg
     5281 aaaccatcga gcacgagtcc gagcaccgcg ccggtgtggc cgtgttcggc gagcacggcg
     5341 gcgaggtggg cgtggtggtg ctggaccgga atcagcggca ggtcgtgccg ggccgcgtat
     5401 tcgctggcga aatgcgcggc ggccgagtcc ggatgcaggt cgcaggcgac gcgctcgggg
     5461 cggatgtcca gcaccgcgag cagatgcgcg accgtgtcgg ccagcgcccg gcgtgcggcg
     5521 gcgctgccga gatcgccgag gtgctgcgac agccaggcgc gatcggcgcg cgtcacacag
     5581 acggtgttct tcagctcgcc gccgagcgcg agcaccggcg ggccggccgc cggcaggcgg
     5641 atctcctgcg gcgtccagcc gcgcgcacgg cgcaggaaca ggggcttgcc ggtgcgggcg
     5701 agcagcacgc tgtcatcgac gcgctgcagg atcgcgcggt cgtggccgag cacggcatcg
     5761 gcgatgccgg cgagccgcgt gcaggcctcg gcatcgtcga tcgccagcgg ctcgccggac
     5821 ggattggccg aggtgcagac gaacagcgcg gactgcgcgg ccctcagcca gtcgctgccg
     5881 gccgggcggc cggcggcctc gtggaacagc aggtattgca gcggcgtcgc cggcagcatc
     5941 acgccgaggc ggtcgaggcc gggcgcgatg ccgggcaggt gtgcgtccgc cgcgggcgcc
     6001 ggcagcagca cgatcggccg ctgcgggctt tcgagcagcg cgcgcgccgg cgcatctgtc
     6061 gaaacaagac cagcgagcga ggcggggttt gcggcaagca gcgcgaaggg tttgccgggt
     6121 ctgcgcttgc gccggcgcag cccggcgacc gcggcggcat tgcgcgcatc gcacatcaga
     6181 tgaaaaccgc cgaggccctt gaccgcgacg atgccgccgg cacgaaacaa ggccagcgtc
     6241 gcggcgagcg gatcgccctc gacctccgcg cggctgccgt cgggccgctg cagccgcagc
     6301 cgcggcccgc aggccgggca ggccagggct tcggcgtgaa aacgccggtc ggccggattt
     6361 tcgtattcct cacggcagct tgcgcagagc gcaaagccgg cgagcgcggt gttcgggcga
     6421 tcccagggga gtctcgcggc gatcgtgtag cgcgggccgc agtgcgtgca ggtcgtgaac
     6481 ggatagcgcc agcgccttcc ggccggatcg aaggtctcgg cgaggcagtc cgcgcagacc
     6541 gcggtgtcgg cgccgatgcc ggtgcgcacc ggcccgcggc cgctgtcgag gatcgcgaag
     6601 ccatcggagc cggcgagcgg tgccagcgtc tgcgcctcga tcgcgtcgag ccgcgcgagc
     6661 ggcggcggca cggcgagcgc ggcacgaaac aaggccagcg ccggcgccgg tccctgcacc
     6721 tcgatcgcga cgccgtcgcc gtcgttgcgc acccagccgg cgaggccgtg ctcgtgcgcg
     6781 agccgccaga tccacggccg caagccgacg ccctgcacca gaccgcgcac ctgcagcgcg
     6841 agccgggcgg tcggggcggg cacgggaatg ttcatgagcg ggccgcttca gcccgccgcg
     6901 accggcgccg gcgtgcgcag ccacgccagc caggcgtcga gcccttcgcc ggtcttcgcc
     6961 gatacgctca gcaccggcgc gcgcggattg acgcggcgca ggcagtcgag cgcgtattcg
     7021 agatcgaagt cgaggtgcgg cagcaggtcg gacttgttca gcagcacgag gctcgccgct
     7081 gcgaagatgt ccggatattt cagcggcttg tcctcgccct cggtgaccga caggatcgcg
     7141 accttcgccg cctcgccgag atcgaaggcc gccgggcaga ccaggttgcc gacgttctcg
     7201 atgaacagca gcgtgcccgg atcgagcggc aggcgcggca gcgcgcgcgc gatcatgtcg
     7261 gcatcgaggt ggcagccctt gccggtgttg atctgcacgg ccgg
//
LOCUS       DAOQIE010000201         7272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5370_length_7272_cov_6.627685, whole genome shotgun sequence.
ACCESSION   DAOQIE010000201 DAOQIE010000000
VERSION     DAOQIE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_7272_cov_6.627685"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK144_12505"
     CDS             <1..1038
                     /locus_tag="PK144_12505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF66593.1"
                     /translation="EPERPADARLRSVRGGALPLYRGLQFGYPAARRLRADWTAARPD
                     LVHVATEGPLGWSALRAAHALGLPVVSSFHTHFHSYTRHYGLALLAAPIRAWLRHFHR
                     RCALTLAPTAALAAELAEAGFGRTGVLGRGIDTARFDPAHRDPRLRAAWGAVDDAPVV
                     LGVGRLAPEKNWALVLQAWAAIRSAQPRARLVLAGEGPMRARLRAALPEASFCGELPP
                     EQLAAIYASADLFLFPSLTETWGNVTAEAMASGLAVVAYRRAGAAELIRDGVSGALAE
                     PGDAAGFIGAALALAADRERCRRLGAAARQVTLGRGWDAVVLGLERSYHDVLNGRHGH
                     GQRALDVRFDA"
     gene            1031..1594
                     /locus_tag="PK144_12510"
     CDS             1031..1594
                     /locus_tag="PK144_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF66594.1"
                     /translation="MPELELPAPLPARLPAPLLRFGAAELAWCRRCNALSGRHFVERG
                     FALVSRLGDGVFWYGLMLLLLAVDGLAGVGGVTRMLAAGGLGLVLYKGLKRSTRRLRP
                     CARSTAIRVTVAPLDEYSFPSGHTLHAVAFTITACAQHPGLVWALAPFALLVAASRLV
                     LGLHYPSDVLAGALLGALCASLALLLP"
     gene            1791..4058
                     /locus_tag="PK144_12515"
     CDS             1791..4058
                     /locus_tag="PK144_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNF66595.1"
                     /translation="MRFRFPVVIIDEDFRSENASGLGIRALAKAIEDEGMEVLGVTSY
                     GDLSSFAQQQSRASAFILSIDDEEFSSPEAAGVAVGQLRAFVEEIRFRNADIPIFLYG
                     ETRTTRHIPNSVLRELHGFIHMFEDTPEFVARYIIREAKNYLDSLPPPFFRALTHYAQ
                     DGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAA
                     SERNAARIFNADHLFFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSILHAITMTGA
                     IPVFLTPTRNHYGIIGPIPLKAFKPDNIRKKIEANPFARAALEKHPDRKPRILTLTQS
                     TYDGVLYNVEMIKDMLGDTVDNLHFDEAWLPHAAFHPFYRDMHAIGTDRPRSKQALVF
                     ATHSTHKLLAGLSQASQIVVQDSEAKKLDRHRFNEAYLMHTSTSPQYAIIASCDVAAA
                     MMEPPGGTALVEESISEALEFRRAMRAVEEEFDADSWWFKVWGPDRLAEEGIGEREDW
                     ILRPEDRWHGFAKLTDGYNMLDPIKATIITPGLDVDGSFAERGIPAAIVTKYLAEHGI
                     IVEKTGLYSFFIMFTIGITKGRWNTLVTELQQFKDDYDRNQPLWRVLPEFVARFPQYE
                     RIGLRDLCRQIHGVYRDNDIARLTTEMYVSNIEPAMKPTDAFAAQVHEEIDRVPIDEL
                     EGRITSILLTPYPPGIPLLIPGERFNRTIMRYLQFARDFNARFPGFETDIHGLVTEDV
                     DGVLHYYVDCVQQSG"
     gene            4169..5761
                     /locus_tag="PK144_12520"
     CDS             4169..5761
                     /locus_tag="PK144_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HNF66596.1"
                     /translation="MLSTANLTMQFGPKPLFENVTVKFGAGNRYGLIGANGCGKSTLM
                     KILGGDLESTNGIVMREPNTRLGKLRQDQFAYENLSVIDTVILGHEELAAVKTERDAI
                     YALPEMSEDDGMRVADLEVRFAELDGYTAEARAGELLLGLGIEEALHWGPMSAVAPGW
                     KLRVLLAQALFSDPDVLLLDEPTNHLDINTIRWLEDVINARNSTMIIISHDRHFLNSV
                     CTHMADLDYGELRVYPGNYDDYMTASTQARERLLSDNAKKKAQIAELQTFVSRFSANA
                     SKARQATSRARQIEKIKLEEVKPSSRVSPYIRFEQYKKLHRQAVTLESVSKGYDGNPP
                     LFKKLSLRIDAGERVAIIGPNGAGKTTLLRCLMQELEPDAGSVKWAEGADIGYYAQDH
                     ASDFADEATLFEWMACWTKGDDQLVRAALGRMLFGNDEIGKSVRVLSGGEQGRMLFGK
                     LTLTRPNVMVLDEPTNHLDMESIEALNLALENYPGTLIFVSHDREFVSSLATRILEFT
                     PRGLVDFTGSYEDYLRSQGIGG"
     gene            5975..6610
                     /locus_tag="PK144_12525"
                     /pseudo
     CDS             5975..6610
                     /locus_tag="PK144_12525"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
     gene            6623..6892
                     /locus_tag="PK144_12530"
                     /pseudo
     CDS             6623..6892
                     /locus_tag="PK144_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            complement(7139..>7272)
                     /locus_tag="PK144_12535"
     CDS             complement(7139..>7272)
                     /locus_tag="PK144_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66597.1"
                     /translation="MIGDDFDFDAGVGVCGKNGQSVPVGVGQPSLKIDGLTVGGTAL"
BASE COUNT         1109 a         2585 c         2486 g         1092 t
ORIGIN      
        1 gagcccgagc ggcccgccga tgcgcggctg cgcagcgtgc gcggcggggc gctgccgctg
       61 taccgcggcc tgcagttcgg ctacccggcg gcccgccggc tgcgcgcgga ctggacggcg
      121 gcgcggcccg atctcgtaca cgtcgccacc gaaggcccgc tcggctggtc ggcgctgcgc
      181 gctgcgcacg cgctcgggct gccggtggtg tcgagcttcc acacgcactt ccacagctac
      241 acccgccatt acgggctggc gctgctcgcg gcgccgatcc gggcctggct gcggcacttc
      301 caccggcgct gcgcgctgac gctggcgccg accgcggcgc tggccgccga gctggccgag
      361 gccggcttcg gccgcaccgg cgtgctgggg cgcggcatcg ataccgcgcg cttcgacccg
      421 gcgcaccgtg acccgcggct gcgcgcggcc tggggcgcgg tcgatgacgc gccggtcgtg
      481 ctcggcgtcg gccggctggc gccggagaag aactgggcac tggtgctgca ggcctgggcg
      541 gcgatccgct cagcgcagcc gcgtgcgcgg ctggtgctgg ccggcgaggg gccgatgcgt
      601 gcgcgtctgc gcgcggcgct gcccgaggcg agcttctgcg gcgaactgcc gccggagcag
      661 ctcgccgcga tctacgcgag cgccgacctg ttcctgtttc cgagcctgac cgagacctgg
      721 ggcaacgtca ccgccgaggc gatggcgagc gggctggcgg tggtcgcgta ccgccgcgcc
      781 ggggccgccg agctgatccg ggacggcgtc agcggcgcgc tggccgagcc cggcgacgcc
      841 gcgggcttca tcggtgccgc gctggcgctc gcggccgacc gggagcggtg ccggcggctc
      901 ggggccgcgg cgcggcaggt gacgctcggt cgcggctggg acgcggtcgt gctcggtctg
      961 gagcgcagtt accacgatgt gctgaacggg aggcatggcc atggccagcg tgcgctcgat
     1021 gtccgcttcg atgcctgaac ttgaactccc tgcgccgctg ccggcgcgcc tgccggcgcc
     1081 gctgctgcgc ttcggggccg ccgagctggc ctggtgccgg cgctgcaatg cgctgtccgg
     1141 ccgccacttc gtcgagcgcg gcttcgcgct ggtgtccagg ctcggcgacg gcgtgttctg
     1201 gtacgggctg atgctgctgc tgctcgccgt cgacgggctg gccggtgtcg gcggcgtgac
     1261 gcgcatgctc gcggccggcg ggctggggct ggtgctctac aaggggctca agcgcagcac
     1321 gcgccggctg cgcccgtgcg cgcgctcgac ggcgatccgc gtgacggtcg cgccgctcga
     1381 cgagtacagc ttcccctcgg ggcacacgct gcacgccgtg gcgttcacga tcaccgcctg
     1441 cgcgcagcac ccggggctgg tctgggcgct ggcgccgttc gcgctgctgg tggccgcctc
     1501 gcggctggtg ctcggcctgc actacccgag tgacgtgctc gccggggcgc tgctcggcgc
     1561 gctgtgcgcg tcgctggccc tgctgctgcc ctgaacgcag cggcgcctgc cgcggtcgga
     1621 gcggattcgg ggccggggca caacggccgg acacggcgca aggtcttgtt catcctgcga
     1681 ttgctgcgag cgctccgggc gcgcgcaggc atcgcgcgtg catgctacag tgcgcgccct
     1741 tcgtcgcccg gtcgcatccg cccgtcccgc cgtttcgcag gagaggctcc atgagattcc
     1801 gttttcccgt cgtgatcatc gacgaagact tccgctccga gaacgcgagc ggcctcggca
     1861 tccgggccct ggccaaggcc atcgaggacg agggcatgga agtgctcggc gtcaccagct
     1921 acggcgacct gtcgagcttc gcgcagcagc agtcgcgcgc cagtgccttc atcctgtcga
     1981 tcgacgatga ggaattcagc tcgccggagg ccgccggcgt cgcggtcggg cagctgcgcg
     2041 ccttcgtcga ggaaatccgc ttccgcaacg ccgacatccc gatcttcctg tacggcgaga
     2101 cgcgcacgac gcgccacatc ccgaacagcg tgctgcgcga gctgcacggc ttcatccaca
     2161 tgttcgagga cacgccggaa ttcgtcgcgc gctacatcat ccgcgaggcg aagaattacc
     2221 tcgactcgct gccgccgccg ttcttccggg cgctgacgca ctacgcgcag gacggctcgt
     2281 attcgtggca ctgcccgggg cattcgggcg gcgtggcctt cctgaagagc ccggtcgggc
     2341 agatgttcca ccagttcttc ggcgagaaca tgctgcgtgc cgatgtctgc aacgccgtcg
     2401 acgaactcgg gcaactgctc gatcacaccg gcccggtcgc ggccagcgag cgcaatgccg
     2461 cgcgcatctt caatgccgat cacctgttct tcgtgaccaa cggcacctcg acctcgaaca
     2521 agatcgtctg gcactcgacg gtcgcgcccg gcgacatcgt cgtcgtcgac cgcaactgcc
     2581 acaaatccat cctgcacgcg atcacgatga ccggcgcgat cccggtgttc ctgacgccga
     2641 cgcgcaatca ctacggcatc atcggtccga tcccgctgaa ggcgttcaag cccgacaaca
     2701 tccgcaagaa gatcgaggcc aacccgttcg cgcgcgccgc gctcgaaaag cacccggacc
     2761 gcaagccgcg catcctgacg ctgacgcagt cgacctacga cggcgtgctc tacaacgtcg
     2821 agatgatcaa ggacatgctc ggcgacaccg tcgacaacct gcatttcgac gaggcctggc
     2881 tgccgcacgc ggcgtttcat ccgttctacc gcgacatgca cgcgatcggc accgaccggc
     2941 cgcgctcgaa gcaggcgctg gtgttcgcga cgcattcgac gcacaagctg ctcgccggcc
     3001 tgtcgcaggc ctcgcagatc gtcgtgcagg attccgaggc caagaagctc gaccgccacc
     3061 gcttcaacga agcctacctg atgcacacct cgacctcgcc gcagtacgcg atcatcgcga
     3121 gctgcgacgt ggccgcggcg atgatggaac cgccgggcgg cacggcgctg gtcgaggaat
     3181 cgatcagcga ggcgctcgaa ttccgccgcg cgatgcgcgc ggtcgaggag gaattcgacg
     3241 ccgattcgtg gtggttcaag gtctggggac cggaccggct ggccgaggag ggcatcggcg
     3301 agcgcgagga ctggatcctg cgccccgagg accgctggca cggcttcgcg aagctgaccg
     3361 acggctacaa catgctcgac ccgatcaagg cgacgatcat cacgcccggg ctcgatgtcg
     3421 acggcagctt cgccgagcgc ggcatcccgg cggcgatcgt gaccaagtat ctggccgagc
     3481 acggcatcat cgtcgagaag accgggctgt actcgttctt catcatgttc acgatcggca
     3541 tcaccaaggg ccgctggaac acgctggtca cggagctgca gcagttcaag gacgactacg
     3601 accgcaacca gccgctctgg cgcgtgctgc cggagttcgt cgcgcgcttc ccgcagtacg
     3661 agcgcatcgg cctgcgggat ctctgccggc agatccacgg cgtctaccgc gacaacgaca
     3721 tcgcgcgcct gaccaccgag atgtacgtct cgaacatcga gccggcgatg aagccgaccg
     3781 atgccttcgc cgcgcaggtg cacgaggaga tcgaccgcgt gccgatcgac gagctcgaag
     3841 gccgcatcac cagcatcctg ctgacgccgt acccgcccgg catcccgctg ctgattccgg
     3901 gcgaacgctt caaccgcacg atcatgcgct acctgcagtt cgcgcgggat ttcaatgccc
     3961 gcttcccggg cttcgagacc gacatccacg gcctcgtgac cgaggatgtc gacggcgtgc
     4021 tgcattacta cgtggactgc gtgcagcagt cgggctgaag caccgttccc gcctgccatc
     4081 gccggcccgc gcgtgcgggc cggcgatttt tcatttatgg ctttcccctg atccatgcgc
     4141 gcccgccgcg ccgcgagtga ccgacacgat gctttccacc gccaacctca cgatgcagtt
     4201 cgggccgaag cccctgttcg agaacgtcac cgtcaagttc ggagccggca accgctacgg
     4261 cctgatcggc gccaacggct gcggcaagtc gacgctgatg aagatcctcg gcggcgatct
     4321 ggagtcgacc aacggcatcg tcatgcgcga gccgaacacg cgcctcggca agctgcgcca
     4381 ggaccagttc gcctacgaga acctgagcgt catcgacacc gtgatcctcg gccacgagga
     4441 gctggcggcg gtgaagaccg agcgcgacgc gatctacgcg ctgcccgaga tgagcgagga
     4501 cgacggcatg cgcgtcgccg acctcgaggt gcgcttcgcc gaactcgacg gctacaccgc
     4561 cgaggcgcgc gccggcgagc tgctgctcgg cctcggcatc gaggaggccc tgcactgggg
     4621 gccgatgagc gcggtcgcgc cgggctggaa gctgcgcgtg ctgctcgcgc aggcgctgtt
     4681 ctcggacccc gacgtgctgc tgctcgacga gccgaccaac cacctcgaca tcaacaccat
     4741 ccgctggctg gaagacgtga tcaacgcgcg caacagcacg atgatcatca tttcccacga
     4801 ccgccacttc ctgaacagcg tctgcacgca catggccgac ctcgactacg gcgagctgcg
     4861 cgtctacccc ggcaactacg acgactacat gaccgcctcg acgcaggcgc gcgagcggct
     4921 gctgtccgac aacgccaaga agaaggcgca gatcgccgag ctgcagacct tcgtcagccg
     4981 cttctcggcc aacgcctcga aggcgcggca ggcgacctcg cgcgcacgcc agatcgagaa
     5041 gatcaagctc gaagaggtca agccgtcgag ccgcgtcagc ccctacatcc gcttcgagca
     5101 gtacaagaag ctgcaccgcc aggcggtgac gctcgaatcc gtcagcaagg gctacgacgg
     5161 caatccgccg ctgttcaaaa agctgtcgct gcgcatcgac gccggcgagc gcgtcgcgat
     5221 catcggcccg aacggcgccg gcaagaccac gctgctgcgc tgcctgatgc aggaactcga
     5281 acccgacgcc gggtcggtca agtgggccga gggcgccgac atcggctatt acgcgcagga
     5341 ccacgcctcg gacttcgccg acgaggcgac gctgttcgaa tggatggcgt gctggaccaa
     5401 gggcgatgac cagctcgtgc gcgccgcgct cgggcgcatg ctgttcggca acgacgagat
     5461 cggcaaatcg gtcagggtgc tctcgggcgg cgagcagggc cgcatgctgt tcggcaagct
     5521 gacgctgacg cggccgaacg tcatggtgct cgacgagccg accaaccacc tcgacatgga
     5581 atcgatcgag gcgctgaacc tcgcgctcga gaactacccc ggcacgctga tcttcgtcag
     5641 ccacgaccgc gagttcgtgt cctcgctggc gacgcgcatc ctggagttca cgccgcgcgg
     5701 cctcgtcgac ttcaccggca gctacgagga ctacctgcgc agtcagggca tcggcggctg
     5761 agccggcgcg atggttttgt gaagccgcat gaaagcgggg ctaaagccgc ggcgccggtg
     5821 acggatattg acggatagca cggggatgcg tgccgggcct gcagcaggcg ggagggcggc
     5881 atccgcgtct tccgtctgca agcccgccga ggccgccatg cctgccgaag tccgtttccc
     5941 gtccgaaagc cttccgctgc ccgagccgcc cgcgatgtcc ggggcatccg ggtccgtgcc
     6001 ggtgcgcgtg ctcgccggcg atggccgggg cagcagcttc agtgccgccg atttccgctt
     6061 cgacggccag ccggcggcgc tggcgatcgc cttcgtgtcg gcgcacctcg acttcgccgg
     6121 cgtctgctcg cggctcgcct cggccgccgg cgcggtgccg ctgctcgcgg tcagcaccgc
     6181 cggcgagctg tgctcggccg gtggcggtgg cggtctgtac cggccggcgg ctgcgcacgg
     6241 cgccggcgtc atcgtgcagg tgttcgcacc ggcgctgttc gcgcaggtca gcgtgcaggc
     6301 ggtggcgctg ccgaacgacg acatccgccg cggcgcaccg acgctcgatc gcgaggcgcg
     6361 cgtgcagcgc atcgccggcg aactcgcgcg cgtgcaggtg ccgttcgcga tcgatgcgcg
     6421 cgacacgctg gcgctcgcct tcgtcgacgg cctctcggcc tgcgagaact acctgatgga
     6481 ggccgtctac cgcagcgggc gcttcccgtg cctgttcgtc ggcggctcgg ccggcggcaa
     6541 gctcgatttc cagcacacct ggctgtacga cggccggcgc gtgctcgaaa accacgcgct
     6601 gctcgcgttc gtgaagatgg cgccgggcat gcgctacgcg ctgccccacg cccgcatcat
     6661 gatgcaccag ccctcgggcg gcgtccgcgg gcaggcgtcc gacatcgcga tccaggccga
     6721 gcagatggcg tacacgaaga agctcctcgc ggagcgcatc gcggcgcaca ccggccagac
     6781 ggtcgagcag atcgagaccg actccgagcg cgatcgctgg ttcaccgcca ccgaggcgcg
     6841 cgcctacggc atcatcgaca aggtgatcct gcgccgcggc gagatggcct gacgccaccc
     6901 gccccggccg gggtcgcgac gggggtcgcg tccggggtct gggcctgggc ggccgtcgcg
     6961 agcacggcga aagcagccgt ggcggcggcg aatcggatct gcaaggtcgg gcgcccctcc
     7021 aaggcgtccc cggacggtcc acggcccggg gtcggccaag gcatagcgac gccgccccgc
     7081 caggcacgtt acaaatcgga aaaacgccgg ggcggagttt gccccggtcc ggcttgctct
     7141 agagcgccgt gccgcccacg gtcaggccgt cgattttcag cgagggctgt ccgaccccga
     7201 cgggaaccga ctgtccgttc ttgccgcaga cgccgacacc cgcgtcgaag tcgaaatcat
     7261 caccgatcat gg
//
LOCUS       DAOQIE010000202         7240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5410_length_7240_cov_5.845651, whole genome shotgun sequence.
ACCESSION   DAOQIE010000202 DAOQIE010000000
VERSION     DAOQIE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_7240_cov_5.845651"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..811)
                     /locus_tag="PK144_12540"
     CDS             complement(185..811)
                     /locus_tag="PK144_12540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNF66598.1"
                     /translation="MNAAEPFVASETALLFVDPYNDFISEGGKFWERIREVAESRDLI
                     AHLQQIVSAARRAGVRLFYVPHRQWQPGDFEDWDHPTPYQLGAARFRMFEKGSWGGEW
                     RSEFAPQPGDVIAKEHYASSGFANTDLDFLLKQHRIRRVIVIGLLANTCIETTARFAT
                     ELGYHTTIVTDATAAVSEACMHAAHELNGPTYAHAILTTAEVVAALAG"
     gene            complement(1020..2396)
                     /locus_tag="PK144_12545"
     CDS             complement(1020..2396)
                     /locus_tag="PK144_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNF66599.1"
                     /translation="MSHTTRFWPQRLRSADGPAYLALADLIADDLRSGRLKSGDRLPP
                     LRELAAALGLNYSTVARGLAEARKRGLIDSRVGSGTYIRGAAPALPLRDGNSADMTMN
                     LPPEPDDPALLAQLQDSTAAAMRAADPWALLRYQDFGGSPQQREAGAHWLHTRFPEAR
                     AGQLLVCPGVHAVLYALLSQLARPGDTICVESLSYPGIKAIAAQLGLRLLALADDGEP
                     CAAALRDACRTAAPKALYLNPTLHNPSTRTLSRSRREALAEVALAYSLPIIEDDAYGR
                     LVRTAPAAFASLAPSLTYYVTGLSKTVGAGLRIAYVHAPGQRPMQRLAGALRALTVMA
                     SPLTTAIATRWIDDGTAARLVEAIRDESAQRLQLAARLLAAHPLRAHPEGFHVWLPLP
                     AGFDANVLAAWLRERGVPAVAAAAFSTDAQPPDALRVNLGGPGTRAQCAAGLQLVAEA
                     LARPPSRS"
     gene            2809..3291
                     /locus_tag="PK144_12550"
     CDS             2809..3291
                     /locus_tag="PK144_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572 family nitrogen starvation response
                     protein"
                     /protein_id="HNF66600.1"
                     /translation="MPAVTRPAHATGEYLVDYEEKVFEDVKAAPGEKALVTFHTVAFE
                     GSVGLVNLLQATRLQRKGYETTILLYGPGVTLGVQRGFPTLGDEAFAGHQNYAKQLTR
                     FIQEGGKVYACRFALQALYGHGEGALVEGIRPIHPLDVLDLMLLHRKAGAVILDTWTL
                     "
     gene            3302..4375
                     /locus_tag="PK144_12555"
     CDS             3302..4375
                     /locus_tag="PK144_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitriliase"
                     /protein_id="HNF66601.1"
                     /translation="MSPPRTVRAAAVQISPVFESADGTLGKVCDAIDEAALRGVELIV
                     FPETFVPYYPYFSFVRPPVQAGREHLRLHALSPTVPGPLTERVSERARRHGMVVVLGI
                     NERDHGTLYNAQLIFDADGTLLLKRRKITPTYHERMIWGQGDGAGLRPVDTRVGRVGA
                     LACWEHYNPLARYALMAQHEEIHCAQFPGSLVGPVFAEQMDVTIRHHALESGCFVVNA
                     TGWLTDAQIEAITRDPALQPALRGGCNTAIVSPEGVHLAPPLREGEGMVVADLDFALI
                     HKRKRMMDSVGHYARPELLSLCLDDRPARPLHSAGAAALSTPSFLSGVPSHEPARHPA
                     AAAGAVEEPGTADGAAVRGSAPA"
     gene            4281..5405
                     /locus_tag="PK144_12560"
     CDS             4281..5405
                     /locus_tag="PK144_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HNF66602.1"
                     /translation="MSLPATLPPLPALSKNRELLTELQSAGLHLLDGGLSGNSRRGGA
                     GPSDHQALSLDGHTLMVPTHTAAVWQSPFTATRDADGRGTLLREGRPLARIDFVQRPR
                     FHALTTADGIPYAQIATLHGRDVLASTVLQTCIRYQSRRKTCQFCAIGQSLAAGRTLA
                     RKTPEQLAEVAAAAVRLDGVRQMVLTTGTPNATDRGAAVLADSARAIRAAVDLPIQAQ
                     CEPPDDPRWLERLRAAGVDTLGMHLEAVTPEVRARIMPGKATVPLAVYWQAFERAVEV
                     FGRGQVSTYLLAGLGDPPAAILAACERLIALGVYPFVVPFVPIAGTPLEDHPAPSAAF
                     MRALLEPLGAMLEAAGLRAADSKAGCGRCGACSTLSLYGG"
     gene            5512..6099
                     /locus_tag="PK144_12565"
     CDS             5512..6099
                     /locus_tag="PK144_12565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF66603.1"
                     /translation="MAREAGKNAPAPACDRHDARPLPVTVALRVRLANGPAERAGALA
                     LRRAVFCGEQAVFADNDRDAIDDEADFLVAVPLSAGAAAAPVLGTVRIHRDAPRRWWG
                     SRLAVDRLWRGHGRLGAGLIRLAVATANARGCDEFLAHVQAQNEPLFRRLGWITLARE
                     SLHGRAHCLMRADLAAFPPHPDPATGERLLARRAR"
     gene            6096..7109
                     /locus_tag="PK144_12570"
     CDS             6096..7109
                     /locus_tag="PK144_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HNF66604.1"
                     /translation="MNAPPDRESLLALIATLRAGRGYLHKTDIAAAAGTLEAVPGVAG
                     DDGHGLGDDCAVLRVPGGGEGWLLFAMEGLVEELVAQAPWFAGFCSVLVNLSDVAAMG
                     GRALAVADALWARDARHGAEVLAGIGAAARAFGVPVAGGHSNYRAQREQLAVAVTGHA
                     RRLLTSFDARPGDRLLMAVDLRGDWVAPWPYWDAATRAPAERLRGDLELLPRLAEDGL
                     SRAAKDISMAGIAGTALMFAECSRAGLVIELDAIPAPPGVEVAHWLAAFPSYGYLLAA
                     APAQAETVLERFRARGIAAADIGGFEPGSGVRLESRRAGAVAGFHDWQQQPFIRPPAT
                     GSG"
BASE COUNT         1011 a         2628 c         2562 g         1039 t
ORIGIN      
        1 gatccgaact ggcagccgac ggcttgacgg gggactgaaa gacggtatct ctcccgcaag
       61 gggagagggg agatcgaagt tcgaccgttg cctgttgatc catccggccc gcggaactgc
      121 tgacttgcgt agataccaac gccgtgacgg gcggggttcg gcagcttgct gcagcgatgc
      181 ggaatcagcc ggccagggcg gcgaccacct cggcggtcgt caggatggcg tgggcgtagg
      241 tcgggccgtt cagttcgtgc gcggcgtgca tgcaggcttc gctgacggcg gccgtcgcat
      301 cggtgacgat cgtcgtgtga tagcccagtt cggtggcgaa tcgggccgtg gtttcgatgc
      361 aggtgttcgc gagcaggccg atcacgatca cccgccggat gcggtgctgt ttcagcagga
      421 aatccagatc ggtattggca aagccgctcg atgcgtaatg ctccttcgcg atcacgtcac
      481 cgggctgcgg tgcaaactcg ctgcgccatt caccgcccca gctgcctttc tcgaacatcc
      541 ggaaacgggc ggcaccgagc tgatagggcg tcggatggtc ccagtcctcg aaatcgccgg
      601 gctgccactg ccggtgcggc acgtaaaaca gccggacacc ggcacggcgc gcggcactga
      661 caatctgttg caggtgtgcg atcagatcgc gggattcggc cacctcgcgg atgcgctccc
      721 agaacttgcc gccctccgaa atgaagtcgt tgtacggatc gacgaacagc agcgcggttt
      781 ccgatgcgac gaaaggttct gcggcattca cggcggttcg ctccttaccc ggatcacccg
      841 gcggacaggt cagcgatgca ggccgcagag catagtcact atcacgatga tatccagtga
      901 cgacgctcac acgggcacct gcggccgcgc gtgcggcgcg actgcgggac attccccctt
      961 cccgcggcgg gagatgggcg tgactgcagc agcagggctg cacacccgcc cgaggcggct
     1021 cacgaccggg acggcggccg ggccagtgct tcggccacga gctgcaggcc ggccgcgcac
     1081 tgtgcgcgcg tgcccggccc gccgagattc acgcgcagcg cgtccggcgg ctgcgcgtcg
     1141 gtgctgaagg ccgcggcggc gacggccggc acgccgcgct cgcgcagcca ggcggccagc
     1201 acgttcgcgt cgaagcccgc gggcagcggc agccagacat gaaagccttc gggatgcgcg
     1261 cgcagcggat gcgcggccag caggcgcgcg gcgagctgca ggcgctgcgc ggactcgtcg
     1321 cggatggcct cgaccagtct cgcggccgtg ccgtcgtcga tccagcgtgt ggcgatcgcc
     1381 gtcgtcagcg gcgaggccat gaccgtcagc gcacgcagcg ccccggcgag ccgctgcatc
     1441 gggcgctgtc ccggcgcatg cacgtaggcg atgcgcaggc cggcgccgac cgtcttcgaa
     1501 agcccggtca cgtagtaggt cagcgacggt gcgaggctcg cgaaggcggc cggggccgtg
     1561 cgcaccagcc ggccgtaggc gtcgtcctcg atgatcggca ggctgtaggc cagcgccact
     1621 tcggccagcg cctcgcgccg gctgcgcgac agcgtgcgcg tgctcgggtt gtgcagcgtc
     1681 gggttcaggt acagcgcctt cggcgccgcg gtccggcagg catcgcgaag cgccgccgcg
     1741 cagggttcgc cgtcatcggc gagcgcgagc aggcgcaggc cgagctgggc ggcgatggcc
     1801 ttgatgccgg gatagctcag cgactcgacg cagatggtgt cgccgggccg cgcgagctgg
     1861 ctcagcagcg cgtagagcac cgcatgcacg ccggggcaga ccagcagctg cccggcgcgc
     1921 gcctccggaa agcgcgtgtg cagccagtgc gcgccggcct cgcgctgctg cggactgccg
     1981 ccgaagtcct gatagcgcag cagcgcccag ggatcggcgg cgcgcatcgc cgcagccgtg
     2041 ctgtcctgca gctgcgcgag cagcgcgggg tcatcgggct ccggcggcag gttcatcgtc
     2101 atgtcggcgc tgttgccgtc gcgcagcggc agcgccggcg ccgcaccgcg gatgtaggtg
     2161 ccgctgccga cgcggctgtc gatcaggccg cgcttgcgcg cctcggcgag gccgcgcgcc
     2221 accgtcgaat agttgagccc gagggccgcg gccagctccc gcagcggcgg cagccggtcg
     2281 ccggatttca gccggccgct gcgcagatcg tcggcaatca ggtcggcgag cgcgagatac
     2341 gccggtccgt cggccgaacg caggcgctgc ggccagaaac gcgtggtgtg ggacatggca
     2401 gccggactcc ggaatgcagg cggtttccgc aggaaatcgc acagggccgt acacgccgcc
     2461 gagtgtaatc cggattctta tggtcacgcc cccggaaacc ggaatggatt gatcgcagat
     2521 tgaatgcatc gggccagata ccggagcctg ttcatggtta tgttttttta atcttcgcac
     2581 tgtccggagg cgcaatgcct gcgcatggcg cgtaaatccc ggtgattgat aattgatcgg
     2641 caggctgcgc ggcgatcgat catcgcccgg ttccggattc gcacctgcat ttgcaggcag
     2701 tggccggcgg cttcatcgca gattgatcgc attgcctgcc aaagcgatgg cacggttgct
     2761 gcgagtcgaa tcctgccgat cccggctcga attcaccgga gaatcccgat gcccgcagtc
     2821 acccgccccg cccatgccac cggcgaatat ctggtcgatt acgaagaaaa ggttttcgag
     2881 gatgtgaaag ccgcgcccgg cgaaaaggcg ctggtcacct tccataccgt tgccttcgaa
     2941 ggctcggtcg gtctggtcaa cctgctgcag gcgacgcgcc tgcagcgcaa gggctacgag
     3001 accacgatcc tgctgtacgg ccccggcgtc acgctcggcg tgcagcgcgg cttcccgacg
     3061 ctcggcgacg aagccttcgc cgggcaccag aactacgcga agcaactgac gcgcttcatt
     3121 caggagggcg gcaaggtcta tgcgtgccgc ttcgcgctgc aggcgctgta cggccacggc
     3181 gagggcgcgc tggtcgaggg catccggccg atccatccgc tcgatgtgct cgacctgatg
     3241 ctgctgcacc gcaaggccgg tgcggtgatc ctcgacacct ggacgctctg aggccgtcgc
     3301 catgagcccg ccgcgcacgg tacgtgccgc cgccgtccag atctcgcccg tgttcgagtc
     3361 cgccgacggc acgctcggca aggtctgcga tgcgatcgac gaggctgccc tgcgcggcgt
     3421 cgagctgatc gtgttcccgg agaccttcgt cccctactac ccgtacttct cgttcgtgcg
     3481 cccgccggtg caggcgggcc gcgagcacct gcgcctgcac gcgctgtcgc cgaccgtgcc
     3541 ggggccgctg accgagcgcg tgtcggagcg cgcccggcgc cacggcatgg tcgtcgtgct
     3601 cggcatcaac gagcgcgatc acggcacgct ctacaacgcc cagctcatct tcgacgccga
     3661 cggcacgctg ctgctgaagc gccgcaagat cacgccgacc taccacgaac gcatgatctg
     3721 gggtcagggc gacggcgccg gcctgcggcc ggtcgacacc cgcgtcggcc gggtcggcgc
     3781 gctggcctgc tgggagcact acaacccgct ggcgcgctac gcgctgatgg cgcagcacga
     3841 ggaaatccac tgcgcgcagt tccccggctc gctggtcggg ccggtgttcg ccgagcagat
     3901 ggacgtgacg atccgccacc acgcgctgga gtccggctgc ttcgtcgtca acgccaccgg
     3961 ctggctcacc gacgcgcaga tcgaggcgat cacccgcgat ccggccctgc agccggcgct
     4021 gcgcgggggc tgcaacaccg cgatcgtctc gcccgagggc gtgcacctcg cgccgccgct
     4081 gcgcgagggc gagggcatgg tcgtcgccga cctcgacttc gcactgatcc acaagcgcaa
     4141 gcgaatgatg gattcggtcg gccattacgc ccgccccgag ctgctgtcgc tgtgcctcga
     4201 cgatcgcccc gcccgcccgc tgcacagcgc cggtgccgcc gccctgtcca cgccgtcctt
     4261 cctctccgga gtcccgagcc atgagcctgc ccgccaccct gccgccgctg ccggcgctgt
     4321 cgaagaaccg ggaactgctg acggagctgc agtccgcggg tctgcacctg cttgacggcg
     4381 gactgagcgg caacagccgc cgcggcggtg ccggtccgtc cgaccatcag gcgctgagcc
     4441 tcgacggcca cacgctgatg gtgccgacgc acacggccgc cgtctggcag tcgccgttca
     4501 cggcgacgcg cgatgccgac ggccgcggca cgctgctgcg cgagggccgg ccgctcgcgc
     4561 gcatcgactt cgtgcagcgg ccgcgcttcc acgcgctcac gaccgcggac ggcatcccgt
     4621 atgcgcagat cgccacgctg cacggccgcg acgtgctggc ctcgaccgtg ctgcagacct
     4681 gcatccgcta ccagagccgg cgcaagacct gccagttctg cgcgatcggc cagtcgctcg
     4741 ccgccgggcg cacgctggcg cgcaagacgc ccgaacagct cgccgaggtc gccgccgcgg
     4801 cggtgcggct cgacggcgtg cgccagatgg tgctgaccac cggcacgccg aacgccaccg
     4861 accgcggcgc ggccgtgctc gccgacagcg cccgcgcgat ccgcgccgcg gtcgatctgc
     4921 cgatccaggc ccagtgcgag ccgcccgacg atccgcgctg gttggagcgc ctgcgcgcgg
     4981 ccggcgtcga tacgctcggc atgcacctcg aagcggtcac gccggaggtg cgcgcgcgga
     5041 tcatgcccgg caaggcgacg gtgccgctcg cggtgtactg gcaggccttc gaacgcgcgg
     5101 tcgaggtgtt cggccgcggc caggtcagca cctacctgct cgccggcctc ggcgatccgc
     5161 cggcggccat cctcgccgcc tgcgagcggc tgattgcact cggcgtgtat ccgttcgtcg
     5221 tgcccttcgt gccgatcgcc ggcacgccgc tcgaagacca tccggcaccg tcggcggcgt
     5281 tcatgcgcgc gctgctggaa ccgctcggcg cgatgctcga agccgccggc ctgcgcgcgg
     5341 ccgacagcaa ggccggctgc gggcgctgcg gtgcctgctc gacgctgtcg ctgtacggag
     5401 gctgacatgg acgcatgccc cctgctgccg gcagtccccg gcccgctgcc ggcgccggag
     5461 gccggtcacg cggaaccctt gtacctttca gccccccgcc cctcgcggga catggcccgg
     5521 gaagcaggga agaacgctcc tgcaccggcg tgtgaccggc atgacgcccg gccgctgccc
     5581 gtcacggtcg cgctgcgcgt gcggctcgcg aacggaccgg ccgaacgcgc cggcgcgctg
     5641 gcgctgcgcc gggcggtgtt ctgcggcgaa caggccgtgt tcgcggacaa cgaccgcgac
     5701 gccatcgacg acgaggccga cttcctcgtc gcggtgccgc tgtccgccgg cgccgcagcg
     5761 gcgccggtgc tcggcaccgt gcgcatccat cgcgatgcgc cgcggcgctg gtggggctcg
     5821 cggctcgcgg tcgaccggct ctggcgcggc cacggccggc tcggcgccgg gctgatccgg
     5881 ctggcggtcg cgaccgcgaa tgcgcgcggc tgcgacgagt tcctcgccca tgtgcaggcg
     5941 cagaacgagc cgctgttccg gcgcctcggc tggatcacgc tcgcccgcga aagcctgcac
     6001 ggccgggcgc actgcctgat gcgcgccgat ctcgcggcct ttccgccgca tccggacccg
     6061 gccaccggcg agcgcctgct cgcgcggagg gcacgatgaa cgcgccgccc gaccgggaat
     6121 cgctgctcgc gctgatcgcc acgctgcggg ccgggcgcgg ctacctgcac aagaccgaca
     6181 tcgccgccgc tgccggcacg ctcgaagccg tgcccggcgt cgccggtgac gacggccacg
     6241 gcctcggcga tgactgcgcc gtgctgcgcg tgccgggcgg cggcgaaggc tggctgctgt
     6301 tcgcgatgga ggggctggtc gaggaactcg tcgcgcaggc gccgtggttc gccggcttct
     6361 gcagcgtgct ggtcaacctc agcgacgtgg ccgcgatggg cggccgggcg ctggcggtcg
     6421 ccgatgcgct gtgggcacgc gatgcgcgcc acggcgccga ggtgctcgcc ggcatcggcg
     6481 cggcggcccg cgccttcggt gtgccggtcg ccggcggcca cagcaactac cgcgcacagc
     6541 gcgagcagct cgccgtggcc gtcaccggcc acgcccggcg cctgctgacc agcttcgatg
     6601 cgcgccccgg cgaccggctg ctgatggccg tcgacctgcg cggcgactgg gtggcgccgt
     6661 ggccgtactg ggacgccgcg acgcgcgccc cggccgaacg gctgcgcggc gatctcgaac
     6721 tgctgccgcg gctcgccgag gacggcctga gccgcgcggc caaggacatc agcatggccg
     6781 gcatcgccgg cacggcgctg atgttcgccg agtgctcgcg cgcgggtctc gtcatcgaac
     6841 tcgacgcgat cccggccccg cccggcgtcg aggtcgcgca ctggctggcc gccttcccga
     6901 gctacggcta cctgctcgcg gccgcaccgg cgcaggccga aaccgtgctc gaacgcttcc
     6961 gcgcccgcgg aatcgccgcg gccgacatcg gcggcttcga gccgggcagc ggcgtgcggc
     7021 tcgaaagccg ccgcgccggg gccgtcgccg ggttccacga ctggcagcag cagccgttca
     7081 tccggccgcc ggccaccggt tcgggctgaa cccgccgtac cgcgaacgac cgcgccggct
     7141 gtgccgacac cgatgcccgt cccccgccac ccgaggacca ccccgatgcc cgccgtccac
     7201 taccgcctgc gctggcccga tgccagcgtc atcgagtgct
//
LOCUS       DAOQIE010000203         7227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5425_length_7227_cov_7.131484, whole genome shotgun sequence.
ACCESSION   DAOQIE010000203 DAOQIE010000000
VERSION     DAOQIE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_7227_cov_7.131484"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PK144_12575"
     CDS             complement(<1..533)
                     /locus_tag="PK144_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNF66605.1"
                     /translation="MRTDPPVSRVPVRAHADGRPRLRSLLLALAAIAAGLTGLARSAP
                     ADEAVVPGLRATGDLALIVERAAGRVALAETSGRTLLGRIDGLGDLSHAALVYSRDQR
                     YAFVFGRDGGLSKVDLLTQRVVGRVVQSGNSIGGAISQDGRHVAVANYEPGGIRIFDA
                     ATLAPLADVPAGYGDNGA"
     gene            complement(566..895)
                     /locus_tag="PK144_12580"
     CDS             complement(566..895)
                     /locus_tag="PK144_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF66606.1"
                     /translation="MRIRTLLLPALALGCALASLPATAEPSPARQRELRHLVRQDCGS
                     CHGMTLKGGLGTPLLPSALAGKTDALLVATVLDGRPNTAMPPWRGLLTEAEAVWIVAQ
                     LRAGLPE"
     gene            complement(1177..1659)
                     /locus_tag="PK144_12585"
     CDS             complement(1177..1659)
                     /locus_tag="PK144_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNF66607.1"
                     /translation="MSGPRRLDRLDRRILQQLQVAGRQTWVELAAAVGLSPSPCLERV
                     RRLEKDGVIRGYAALVEPSAVGASLLVFVEISLDYTTPDIFERFRAAVAGLPQIQECH
                     LVSGNFDYLLKARIGDMAEYRELLGLFLRTLPGVRDSRSFVVMETVKETQAVFVPETG
                     "
     gene            1889..3142
                     /locus_tag="PK144_12590"
     CDS             1889..3142
                     /locus_tag="PK144_12590"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNF66608.1"
                     /translation="MKIVVLGAGVVGTTTAWYLAQAGHQVIVLERQPGPALETSYANA
                     GQISPGYSAPWAGPGVPVKAIKWMFMEHAPLVIRPRINPAMWRWMWQMLMNCNAKDYE
                     LNKSRMMRLAEYSRDQFITLRAETGLHYDDRQQGTLQVFRTQAQIDGAAKDIAVLKSL
                     GVPHEVLDRAGCVAAEPALALVSDKIQGGLRLPGDETGDCFQFTNRLAEMAAARGVEF
                     RYRTAIFGLERSGDEITAVLTNRGRETADAVVCALGSYSTKLMQELGYHIPVFPVKGY
                     SITVPVVDPEFAPVSTVMDETHKVAITRLGDRIRVAGTAELTGYDLQLTNERGNTVRH
                     VVTDLFPRGGDVSRAELWTGLRPNTPDGTPIIGRTPLKRLWLNTGHGTLGWTMCCGSA
                     RVLTDQIGGRTPDIDTEGLSMARYG"
     gene            3283..4362
                     /gene="alr"
                     /locus_tag="PK144_12595"
     CDS             3283..4362
                     /gene="alr"
                     /locus_tag="PK144_12595"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNF66609.1"
                     /translation="MRPAHAVIDLAALRHNLARARAAAPGARAIAIVKADGYGHGAAR
                     LLPALADADMLGVACIEEALALRAAGATQPVLLLEGVFSADELPVCAEQDFEIAIHDP
                     LHVEWLERARLATPLRVWLKLDSGMHRLGVRPQQVAPLLARLRVCANVAPPIGVMSHL
                     ACADEPARAETAAQLETFAAATAGLPVRRALSNSAGVLAWPAAHHDWIRPGIMLYGAT
                     PMLGRTGPEDGLKPVMTLSSALISVKRLQAGEPVGYSGAWRCPRDTTIGVVAMGYADG
                     YPRHAPSGTPVLVNGRRTQVVGRVSMDMLCVDLGEQPGARIGDPVTLWGEGLPAELVA
                     EAAGTIAYELFCNLAARVHVRVIDG"
     gene            4554..5384
                     /locus_tag="PK144_12600"
     CDS             4554..5384
                     /locus_tag="PK144_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF66610.1"
                     /translation="MHIRIGYELTWDLPQPTPMILTLHVHPSRRADLIEPDRMQTQPA
                     VLTETYQDGFDNLCTRLVAPAGRFTVRSDALIRDPGTFDRCVPEAQQHPVETLPEETL
                     VFLLGSRYCETERLMPIAWQLFGHIQPGWARVQAICDYVHRHISFGYHHARATRTAWE
                     AHEERIGVCRDFTHLAVAFCRCLNIPARYCTGYLGDIGVPPGGPMDFSAWFEAYIGQH
                     WHTFDPRNNQPRIGRVLIARGRDASDVAISSTFGPNVLSGFRVVTEELKPQPQYAAAS
                     "
     gene            complement(5350..6177)
                     /locus_tag="PK144_12605"
     CDS             complement(5350..6177)
                     /locus_tag="PK144_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66611.1"
                     /translation="MPLPLSPSAAGLLSAFLLVAGNGHAAQITYLDQQRSAAVDAQVR
                     VLDFGHGELQSAVGHDLSAAAPGDFSPFTAGSNVTGLFVSDYGANASADGFANHASTL
                     AADGIRFTGDVAMYAAGSPGSPGIPSVSGLAEVLMGVRFVLDEAATLELSLDSDPNGG
                     FDFRLLGAGASVVWDQTSLYSPATGPQFTFVIHLPLAAGEYRLDATLRAYSSFDNDTG
                     YSATSFGSFALTLPASVIAEPHPAMLLCAGLVATATAMRRRPRPLPLTTPLRTAAAV"
     gene            complement(6238..6639)
                     /locus_tag="PK144_12610"
     CDS             complement(6238..6639)
                     /locus_tag="PK144_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66612.1"
                     /translation="MFLPSAVLSRLPPADRQLAESMHELLRAEIIRQLDARHAAAAAR
                     RNEFLLAATGSSARLPRPVLGTGAGHVPSEHDVSRARALALYRRDDELAELACLFIGQ
                     EALAAHMDPELVRERDPAEELWQQRFGASAV"
     gene            complement(6798..>7227)
                     /locus_tag="PK144_12615"
     CDS             complement(6798..>7227)
                     /locus_tag="PK144_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66613.1"
                     /translation="ALPLLWALVSAVAMLGSAPLSGLSDRIGRRQLIAGGWTLFALVY
                     ALLGWPRLPPAGLWPLAALMGLHLAATEGTAKALVTDLAPAADLGRAFGWYHLISGLC
                     LLPASWLTGTLWAAHGAPLACAWAGGCALLAATLLAFTKH"
BASE COUNT         1130 a         2520 c         2568 g         1009 t
ORIGIN      
        1 gcgccgttat cgccgtaccc ggccggcacg tcggcgagcg gcgcgagcgt cgcagcatcg
       61 aagatgcgga tgccgcccgg ctcgtagttg gcgaccgcga catggcggcc gtcctgcgag
      121 atggcgccgc cgatcgaatt gccggactgc acgacgcgcc cgacgacacg ctgcgtcagc
      181 agatcgacct tgctcaggcc gccgtcgcgg ccgaacacga acgcgtaacg ctgatcgcgc
      241 gaatagacga gtgcggcatg ggacagatcg ccaaggccgt cgatgcgccc gagcagcgtg
      301 cgcccgctgg tctcggcgag cgcgacgcgc ccggcggcgc gctcgacgat cagcgccaga
      361 tcgccggtgg cgcgcagccc cgggaccacg gcttcgtcgg ccggggcgct gcgggccagg
      421 ccggtcaggc cggcggcgat cgccgcgagc gcgagcagca gcgagcgcag ccgcggccgg
      481 ccgtcggcat gcgcacgtac gggaacacgg gagacgggcg ggtcggttcg catcgcggca
      541 gtcctcacgc agggggagcg ttcgatcatt cgggcaggcc ggcgcgcagc tgcgcgacga
      601 tccacacggc ttcggcttcg gtgagcagcc cgcgccacgg cggcatcgcc gtgttcggcc
      661 ggccgtcgag cacggtcgcg accagcagcg catcggtttt gccggccagc gccgagggca
      721 gcagcggcgt gccgagcccg cccttcagcg tcatgccgtg gcaggagccg cagtcctgcc
      781 gcacgaggtg gcgaagctcg cgctggcgcg ccggcgacgg ttcggccgtt gccggcagcg
      841 acgccagcgc gcagccgagc gcgagcgcgg gaagcagcaa ggtgcggatt cgcatggacg
      901 gcggctccat gacagtgcag atgccgcaca ttgcaccgtg ccggagcagc cagcgccctt
      961 gatacccatc aagcggcagg gaactggcgg gcgggcagag gaggtagcag ggagtagggc
     1021 aggaggtaat gccgatcaca tcagaacgct ttgcttttca gcccctcgcc ccgcgtgggc
     1081 gcggggttgg cgaacggggg agaaaaacca tgcgtttacc tgtgatcgct gtaagcggtg
     1141 aagcgggtac gggtgtctgc cggtcgccgg ccgcgttcac ccggtctccg gcacgaacac
     1201 cgcctgcgtc tccttgaccg tctccatcac cacgaagctg cgcgaatcgc gcacgcccgg
     1261 cagcgtgcgc aggaacaggc cgagcagttc gcggtattcg gccatgtcgc cgatgcgcgc
     1321 cttcagcaga tagtcgaagt tgccggaaac caggtggcat tcctggatct gcggcaggcc
     1381 ggcgacggcg gcgcggaagc gttcgaagat gtcgggcgtc gtgtagtcga ggctgatctc
     1441 gacgaacacc agcaggctgg caccgaccgc ggagggttcc accagcgccg cgtagccgcg
     1501 gatgacgccg tccttctcca ggcggcgcac gcgctccaga cacggcgacg gcgacagccc
     1561 gaccgccgcg gccagctcga cccaggtctg gcggccggcg acctgcaact gctgcaggat
     1621 gcgccggtcg agacggtcga gcctgcgcgg tccgctcatt gcaggcattc cggacacggc
     1681 cgcggcgcag gctttgcggt cgcggggcgg gcagtctcga atcttcgggg cattctgctg
     1741 tgcatgaggt tctgatccgg aaaatccact gcagtacggc cggtaacata gtgcatgttg
     1801 ccggtgccga ccttgtatgc cgtcgaagcc gccggttccc ctgtccagtt tcagccggct
     1861 gcgggccggt cacgggagtg tgtttgccat gaagatcgtc gttctgggtg ccggtgtcgt
     1921 cggcacgacc accgcctggt acctcgcgca ggccggccat caggtcatcg tgctggagcg
     1981 ccagcccgga ccggcgctgg agacgagcta cgccaacgcc ggccagatct cgcccggcta
     2041 ctcggcaccg tgggccggtc ccggcgtgcc ggtcaaggcg atcaagtgga tgttcatgga
     2101 gcatgcgccg ctggtgatcc gcccgcgcat caatccggcg atgtggcgct ggatgtggca
     2161 gatgctgatg aactgcaacg cgaaggatta cgagctcaac aagtcgcgga tgatgcggct
     2221 cgccgagtac agccgcgacc agttcatcac gctgcgcgcc gaaaccggcc tgcactacga
     2281 cgaccgccag cagggcacgc tgcaggtgtt ccgcacgcag gcgcagatcg acggtgcagc
     2341 caaggacatc gcggtgctga agagcctcgg cgtgccgcac gaggtgctcg accgggccgg
     2401 ctgcgtggcc gccgagccgg cgctcgcgct ggtcagcgac aagatccagg gcggcctgcg
     2461 cctgcccggc gacgagaccg gcgactgctt ccagttcacc aaccgcctcg ccgagatggc
     2521 cgcggcgcgc ggcgtcgaat tccgctaccg cacggcgatc ttcgggctgg agcggagcgg
     2581 cgacgagatc accgccgtgc tgaccaaccg cggtcgcgag accgccgatg ccgtggtctg
     2641 cgcgctcggc agctattcga ccaagctgat gcaggaactc ggctaccaca tcccggtgtt
     2701 cccggtgaag ggctactcga tcaccgtacc cgttgtcgat ccggagttcg cgccggtatc
     2761 gacggtcatg gacgagaccc acaaggtcgc gatcacgcgc ctcggcgacc gcatccgcgt
     2821 cgccggcacc gccgagctga ccggctacga cctgcagctg accaacgagc gcggcaacac
     2881 cgtgcgccac gtcgtcaccg acctgttccc gcgcggcggc gacgtgagcc gcgccgaact
     2941 ctggaccggc ctgcgcccga acacgccgga cggcacgccg atcatcggcc gcacgccgct
     3001 gaagcgcctg tggctgaaca ccggccacgg cacgctcggc tggacgatgt gctgcggctc
     3061 ggcgcgcgtg ctcaccgacc agatcggcgg acgcacgccg gacatcgaca ctgaagggct
     3121 gtcgatggcg cgctacggct gagggcgctc accgcctgcc ggggcctgcg catgcagtcc
     3181 cccgtcccgt tcacgaaggc gcacgataag cgtcgatgac attccagcgt cgccgccgcc
     3241 tgcgggaggc gacgcaccgt acctgcccgg agtccctgcc cgatgcgtcc cgcccacgcc
     3301 gtcatcgatc tcgccgcgct gcggcacaac ctcgcccgtg cccgcgccgc ggcaccgggt
     3361 gcgcgcgcca tcgccatcgt caaggccgac ggctacggcc acggcgccgc gcgcctgctg
     3421 ccggcactcg ccgatgccga catgctcggc gtcgcctgca tcgaggaggc gctggcgctg
     3481 cgcgccgccg gtgccacgca gccggtgctg ctgctcgaag gcgtgttctc ggccgacgaa
     3541 ctgccggtgt gcgccgagca ggatttcgag atcgccatcc atgacccgct gcacgtcgaa
     3601 tggctggagc gcgcccggct ggccacgccg ctgcgcgtgt ggctgaagct cgacagcggc
     3661 atgcaccgcc tcggcgtgcg tccgcagcag gttgcgccgc tgcttgcgcg cctgcgcgtc
     3721 tgcgcgaacg tcgcgccgcc gatcggcgtc atgagccacc tcgcctgcgc cgacgagccg
     3781 gcgcgcgccg agaccgccgc gcagctcgaa accttcgccg ccgccacggc cggcctgccg
     3841 gtgcgccggg cgctgtcgaa ctcggccggc gtgctggcct ggccggcggc gcatcacgac
     3901 tggatccggc cggggatcat gctctacggc gcgacgccga tgctcgggcg caccggtccc
     3961 gaggacggcc tgaagccggt catgacgctc agcagcgcgc tgatctcggt caagcgcctg
     4021 caggccgggg agccggtcgg ctattccggc gcctggcgct gcccgcgcga tacgacgatc
     4081 ggcgtggtcg cgatgggcta cgctgacggt tatccgcgcc acgcgccgag cggcacgccg
     4141 gtgctggtca acggccggcg cacgcaggtg gtcgggcgcg tgtcgatgga catgctgtgc
     4201 gtcgatctcg gcgagcagcc cggggcgcgc atcggcgatc cggtgacgct ctggggcgag
     4261 ggactgccgg ccgaactcgt cgccgaggcc gccggcacga ttgcctacga acttttctgc
     4321 aacctcgcgg cgcgcgtgca tgtccgcgtg atcgacggct gacgcgaacc ccgccggcag
     4381 cctgcggtcg gaacagtcat gccggcatcc ttgccaatgc cggcaggggc cttgaatgcg
     4441 gcccgccgca ggcgacgggc gcttcatgaa gctccggatc ggtgcgcggc gggtctgtcc
     4501 tttccgccaa tgccggtcgc acctgcgacc gccgagccga gacgctgacc gccatgcaca
     4561 ttcgcatcgg atacgaactc acctgggacc ttccgcagcc gacgccgatg atcctgacgc
     4621 tgcatgtgca tccgtcccgt cgcgccgacc tgatcgagcc ggaccggatg cagacgcagc
     4681 cggccgtgct gaccgagacc taccaggacg gcttcgataa cctctgcacg cggctggtgg
     4741 caccggccgg gcgcttcacg gtgcgcagcg acgcgctgat ccgggacccc ggcaccttcg
     4801 atcgctgcgt gcccgaggcc cagcagcatc cggtcgagac cctgcccgag gagacgctgg
     4861 tattcctgct cggcagccgc tactgcgaga ccgaacggct gatgccgatc gcctggcagc
     4921 tgttcggcca catccagccg ggctgggcac gcgtgcaggc gatctgcgac tacgtgcacc
     4981 ggcacatcag cttcggctat caccatgcgc gcgcgacgcg cacggcctgg gaggcgcacg
     5041 aggagcgcat cggcgtctgc cgtgacttca cgcacctcgc cgtcgcgttc tgccgctgcc
     5101 tgaacatccc ggcgcgctac tgcaccggct atctcggcga catcggcgtg ccgccgggcg
     5161 ggccgatgga cttctcggcc tggttcgagg cctacatcgg ccagcactgg cacaccttcg
     5221 atccgcgcaa caaccagccg cgcatcggcc gcgtgctgat cgcacgcgga cgcgatgcct
     5281 cggatgtcgc gatcagcagc accttcgggc cgaacgtgct gagcggcttc cgggtcgtga
     5341 ccgaggaact caaaccgcag ccgcagtacg cagcggcgtc gtgagcggca agggccgggg
     5401 gcgcctgcgc atggcggttg ccgtcgccac cagacccgcg cagagcagca tcgccgggtg
     5461 cggctcggcg atgacgctcg ccggcagcgt cagcgcgaag ctgccgaagc tggtggcgga
     5521 gtagccggta tcgttgtcga agctcgaata ggcccgcagc gtcgcatcga gccggtattc
     5581 accggcggcg agcggcaggt ggatcacgaa cgtgaactgc gggccggtgg ccgggctgta
     5641 gaggctggtc tggtcccaga ccaccgaagc gccggccccg agcagccgga agtcgaaacc
     5701 gccgttcgga tcgctgtcca gcgacagttc cagcgtggcc gcttcgtcga gcacgaagcg
     5761 cacccccatc agcacttcgg ccagcccgct gacgctcggg atgccgggcg agcccggtga
     5821 gccggcggca tacatcgcca cgtcgccggt gaagcggatg ccgtcggcgg ccagcgtcga
     5881 ggcgtggttc gcgaagccgt cggcgctggc gttggcgccg tagtcggata cgaacaggcc
     5941 ggtgacgttg ctgccggccg tgaacggact gaaatcgccg ggggccgcgg ccgagagatc
     6001 atgaccgacg gcgctctgca gttcgccgtg accgaagtcg agtacgcgca cctgcgcatc
     6061 gactgcggcc gagcgctgct gatcgagata cgtgatctgc gccgcatggc cgttgccggc
     6121 gacgagcagg aaagccgaaa ggaggccggc tgcagaaggc gaaagcggaa gcggcatggt
     6181 ggcggcactg cgcaggaccc gggagcgggt cgcagcagcg tagtgcctgc cggcggttca
     6241 gacggcgctt gcgccgaagc gctgctgcca gagttcttcg gccggatcgc gctcgcggac
     6301 cagttccggg tccatgtgcg cggccagcgc ctcctggccg atgaacaggc aggcgagttc
     6361 ggccagttca tcgtcgcgcc ggtacagcgc cagcgcccgg gcgcgggaga catcgtgttc
     6421 ggacggcaca tggccggcgc cggtgccgag caccggccgc ggcagccgcg cagaactgcc
     6481 ggtggcggcg agcaggaatt cattgcgccg cgcggcggcg gcggcatggc gggcatcgag
     6541 ctggcggatg atttcggccc gcagcagttc gtgcatcgat tcggccagct gacggtcagc
     6601 cggcggcagc cgggacagca cggcggacgg caggaacaat tcccctagcc acgtgctggt
     6661 ccacgagtgg gtgcgatgga ggtattcgcg cagtcgcatc gtgaccgttc cctgtcggat
     6721 gtggaggaag tgaatccgcg tgcctgttgc aggcaccgtt tcaacataac acggctgaat
     6781 gagcgtgccg gcatagatta atgtttggta aacgcgagca gggttgcggc gagcagcgcg
     6841 cagccgccgg cccaggcgca ggcgagcggc gcgccatgcg ccgcccacag cgtgccggtc
     6901 agccatgagg ccggcagcag gcacagcccg ctgatcaggt gataccagcc gaacgcccgg
     6961 ccgaggtccg ccgccggtgc gagatcggtg accagggcct tcgccgtgcc ttcggtcgcg
     7021 gccagatgca gccccatcag tgccgcgagc ggccacagcc ccgccggcgg cagccttggc
     7081 cagccgagca acgcgtagac cagcgcgaac agcgtccagc cgccggcgat cagctggcgc
     7141 cggccgatgc gatccgacag tcccgacagc ggcgccgagc ccagcatcgc caccgccgac
     7201 accagcgccc acagcagcgg cagcgcg
//
LOCUS       DAOQIE010000204         7221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5432_length_7221_cov_7.847056, whole genome shotgun sequence.
ACCESSION   DAOQIE010000204 DAOQIE010000000
VERSION     DAOQIE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5432_length_7221_cov_7.847056"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1805)
                     /locus_tag="PK144_12620"
                     /pseudo
     CDS             complement(237..1805)
                     /locus_tag="PK144_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1938..2435
                     /locus_tag="PK144_12625"
     CDS             1938..2435
                     /locus_tag="PK144_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66614.1"
                     /translation="MVAAAANADPACTLRIEVDRREPAWIRLRSVRPEAPGGCALDTD
                     TLRRTLAAALAAAGPVVTVALGRLVGYPALACGLAAQAAADPGWDRRHGRARDGRSDN
                     TWTAQALAASQPLAGLLPAGWTLQAVSVEKVLKGRPAQQLADCPVEGGGLPFDAQLWL
                     RLRRR"
     gene            complement(2453..3847)
                     /gene="glmU"
                     /locus_tag="PK144_12630"
     CDS             complement(2453..3847)
                     /gene="glmU"
                     /locus_tag="PK144_12630"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNF66615.1"
                     /translation="MSLSSPASPANLHVVILAAGKGKRMVSDLPKVLHAVGGRPMLGH
                     VIASAQRLGAARIVVVHGHGSEQVLARFGTVPGIAWAEQAEQLGTGHAVAQALPALPA
                     DGLALVLYGDVPLVRPESLTPLLAAAAAGGIGLLTVELPDPTGYGRIVRDDGGRVLRI
                     VEEKDADAATKRIREGNTGILAAPLARLRDWVGRLGNANAQGEYYLTDIIEMAVGEGL
                     AVVPHTVAAPEEVQGVNNRVQLAELERFYQRAQAEALMIAGATLADPARIDVRGEVST
                     GRDVQIDVNVVFEGRVVLGDRVRIGANCVLRDCTIADDVEIKPFTLIEQAEVGTRAQL
                     GPYARLRPGTVLAEDTHVGNFVELKNSTLAAGAKANHLSYIGDATVGAAVNIGAGTIT
                     CNYDGVNKSRTVIGDGAFIGSNSSLIAPVTIGAQATVGAGSAIGKDVPDATLAITRAP
                     QKTITSWKRPEKKT"
     gene            complement(3944..4369)
                     /locus_tag="PK144_12635"
     CDS             complement(3944..4369)
                     /locus_tag="PK144_12635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNF66616.1"
                     /translation="MAMTLHVDIVSAEKQIFSGTAEMLVATAEEGELGILPRHSQMLA
                     RLKPGQVRVKLQFGEEQVFYVSGGLLEVQPHTVTVLADTAERAADLDEAKAIEAKRRA
                     EEILAARSDEISLARAQAELAEAVAQLQAIEKLRKLRKA"
     gene            complement(4444..5820)
                     /gene="atpD"
                     /locus_tag="PK144_12640"
     CDS             complement(4444..5820)
                     /gene="atpD"
                     /locus_tag="PK144_12640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNF66617.1"
                     /translation="MSSGKIVQVIGAVVDVEFPRGAVPKIYDALKIDGADLTLEVQQQ
                     LGDGIVRAIALGTSDGLRRGMVANNTGAAINVPVGKATLGRIMNVLGTPIDHQGPVGA
                     ETTWAIHRKAPAYEEQSGSTDLLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGV
                     LQERITSTKKGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIIGEEHYSTARAVQGVLQRYKELKDIIAILGMDELSEEDRQVVARA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDSIRGFKGIIAGEYDHLPEQAFYMVGSIDE
                     AVEKAAKL"
     gene            complement(5890..6756)
                     /gene="atpG"
                     /locus_tag="PK144_12645"
     CDS             complement(5890..6756)
                     /gene="atpG"
                     /locus_tag="PK144_12645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNF66618.1"
                     /translation="MAGAKEIRTKIKSIKNTQKITRAMEMVAASKMRKAQERMYAARP
                     YAEKIRTVISHLAHAHTEYRPPALMDRPDVKRVGYIVISTDRGLCGGLNTNLFKATVQ
                     SMREWQAKGVESELCTVGTKAAGFFRRLGVKVAATVSHLGDTPRIEDLVGSVKVMFDR
                     FREGEIDRIYLVHNVFLNTMTQKPSVELLVPVSARVEKVDAPHRWDYLYEPDATVLLD
                     TLLVRYVESLVYQGLVENVACEMAARMIAMKSASDNAGSLIDELQLVYNKARQAAITQ
                     ELSEIVAGAAAV"
     gene            complement(6769..>7221)
                     /locus_tag="PK144_12650"
     CDS             complement(6769..>7221)
                     /locus_tag="PK144_12650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011040935.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNF66619.1"
                     /translation="IRPAINAGLSVSRVGGAAQTKAVKKLGGGVRLALAQYRELAAFA
                     QFASDLDDATRKQLERGQRVTELMKQKQYSPLSVGEMAISLFAADKGYLDDVPLNKIG
                     DFESALIAYMNANQSELMKKVNETGDYNGEIEAAFAKGVEDFKANHVW"
BASE COUNT         1056 a         2446 c         2524 g         1195 t
ORIGIN      
        1 atgccgccgg ctgcactttc gggaccgttg tcgtaaagca gcatggcagt ggcaggctgc
       61 agcgaggaca acagcagagc cagatatgca taacgtcggg gcagggcggg cggcatgatg
      121 acgagactcc ggtggcaacg gcacccggag cgtagctcct gtcattctgg tcatgaaaat
      181 caatacggta tgggcaaggt ggtgcattcg gtgccgtccg ctggagtctc ggcgcagcgc
      241 cacacccgga accccgcatc cccgtcccag tccggaaact ccgcccgcag cagcgccgcc
      301 gtgcgccgca gtcgctcgcg gcacagcccg gcgaggtccg gagccagtcc gtgctgctcg
      361 cagaacgcga acgctgcgcg ctggccggac ttgcgcgggt cgagcggctc ggggcgctgc
      421 acgagcagga agcgtctgcg gccgccgtcg ccggcgttga gcttcatgac cgcgtgcgcg
      481 gtggtgccgg agccggccca gcagtcgagc acgagatcgt cgccgcccgt gcaccagccg
      541 atcaccgccg cgacgaagtc gaccggcttc ggctggtcga acgggatgcc gagcgtgcgc
      601 agcaaggcgt cgtcggaacc gccgtagggc aggatcgacg gcacgttctc gtacaggttc
      661 tcgtcgaggt agtagatgcg ttgcggctgc accgactcat ccggcccgaa ctcgatccgg
      721 ccggtgtcga gcagccggcg catggtctcg ggcgggttgc gccagccgcg caccggcacc
      781 gggcaggggc ggccggtctg cggatggatc agcggctcga agtactcggc cgacggcgcc
      841 tgcttgttcg gccaggccat cgacaccagc cggtacacgc gcccgtcggc cgacagccgc
      901 cagtacatcg cctcgccgcc ggagagctgc ggctgcgact tcagccaggc acggtagcgg
      961 ctgcgggctt cttcgagcgt cggactcgcg gcgagggcgg tgcgggcggc ggcgagcatc
     1021 tgctgcgcgt ggcgcttggg ccggcgcagc ggttgcctgg cgagcagcgt cgcggcgtcg
     1081 cgcgcgtaca gcagcagcga ttcatgctgg taggcgatgc cgcgcgcgtc gcccttgggg
     1141 ttgcgcttgt cccagaccgc gatgccgagt tcgttggcct cgccgaacag ctcgcgcagc
     1201 agcagcgtca gcgccggctg ctcgtgctcg tcgatgtgga tcgcgagcag gccgtcgtcg
     1261 gcgagcagcg tgcggatcag cgccagccgc ggatacatgc ggctcagcca gtcgccgtgc
     1321 cggcgcgtgc ggtcggcatc cggtgcgccg cggcgcacgc cgccgtggcg gtcggcatag
     1381 atgaaatcgc ggccggtgtt gtacggcgga tcgagatagg cgagcttgac gcgtccggcg
     1441 tagccggtct gcagcagctt cagcacgtcg aggttgtcgc cctcgatgaa gccgttgcgc
     1501 gtcgtcgcct cgtcgaggcc ggcgccgggg cagcgctgca gccggccggc cggtggcgag
     1561 gcgatctgcg cggcggccgc tgcccgttgc ggccagtgca ggccgaagcc gtcggcttcc
     1621 ccgtcggcgg catgcgggcc gagcagccgg cgcagctggg cgaggtcgac gctcgcgagc
     1681 gtccggccct cgacgaggcg ctcgccgacg gcctgcggaa acagcgcgcg cagccgctcg
     1741 atgcgatcgg caagatccgc cgcgctcacg agcgcatccg gacaatcggc gtcaggtcgg
     1801 gacatgggcg ggcgcggggc ggcagcggac cggcggcatt agcgcaggcc gcagcaggcg
     1861 gatcaagcgt cgcgcacgcg gcgggggagg gcggtctgaa cgcacgacaa cacggcttcg
     1921 gcctcgccgg cctgctgatg gtcgcggcag cggcgaatgc cgatccggcc tgcacgctgc
     1981 gcatcgaggt cgatcgccgg gaaccggcgt ggatacgcct gcgcagcgtc cggcccgaag
     2041 ctccgggggg ctgtgcgctc gacaccgata ccttgcgccg cacgctcgcc gcggcgctgg
     2101 ccgctgccgg accggtcgtg accgtcgccc tcgggcggct ggtcgggtat ccggcgctcg
     2161 cctgcgggct ggcggcacag gcggctgccg atcccggctg ggaccggcgc cacgggcgtg
     2221 cccgcgatgg ccgcagcgac aacacctgga cggcgcaggc gctggctgcc tcgcagccgc
     2281 tcgccgggct gctgcccgca ggctggacgc tgcaggcggt gtcggtcgag aaggtgctga
     2341 agggccgccc ggcacagcag ctggcggact gcccggtgga gggcggcggc ctgcccttcg
     2401 atgcgcagct ctggttgcgg ctgcggcggc gctgagccgg ccggcaccgg cgtcaggtct
     2461 tcttttccgg ccgtttccag ctcgtgatcg tcttctgcgg cgcgcgcgtg atcgccagcg
     2521 tggcgtccgg cacgtccttg ccgatcgccg aaccggcgcc gaccgtcgcc tgcgcgccga
     2581 tcgtcaccgg ggcgatcagc gagctgttcg agccgatgaa ggcgccgtcg ccgatcacgg
     2641 tgcggctctt gttcacgccg tcgtagttgc aggtgatcgt gccggcgccg atgttgaccg
     2701 ccgccccgac cgtcgcgtcg ccgatgtagc tcaggtgatt ggccttggcg ccggcggcca
     2761 gcgtcgagtt cttcagttcg acgaagttgc cgacgtgcgt gtcctcggcg agcacggtgc
     2821 cggggcgcag gcgcgcgtac gggccaagct gcgcgcgggt gccgacctcg gcctgctcga
     2881 tcagcgtgaa cggtttgatc tcgacatcgt cggcgatcgt gcagtcgcgc agcacgcaat
     2941 tcgcgccgat gcgcacgcgg tcaccgagca cgacccggcc ctcgaacacg acgttcacgt
     3001 cgatctgcac gtcgcggccg gtggaaactt cgccgcgcac gtcgatgcgc gccggatcgg
     3061 cgagcgtggc gccggcgatc atcagcgcct cggcctgcgc gcgctggtag aagcgctcca
     3121 gctcggcgag ctgcacgcgg ttgttgacgc cctggacctc ctcgggcgcg gcgaccgtgt
     3181 gcggcacgac ggcgagccct tcgccgaccg ccatctcgat gatgtcggtc aggtagtact
     3241 cgccctgggc gttggcgttg ccgaggcgcc cgacccagtc gcgcaggcgc gcgagcggtg
     3301 cggcgaggat gccggtgttg ccttcgcgga tgcgcttcgt ggcggcatcg gcgtccttct
     3361 cctcgacgat gcgcagcacc cggccgccgt catcgcgcac gatgcgcccg tagccggtcg
     3421 ggtccggcag ctcgaccgtc agcaggccga tgccgccggc cgccgccgcc gcgagcagcg
     3481 gcgtcaggga ttcagggcgc accagcggca cgtcgccgta cagcaccagc gccagcccgt
     3541 ccgccggcag cgccggcagc gcctgcgcga cggcgtggcc ggtgccgagc tgctcggctt
     3601 gctcggccca ggcgatgccg ggtaccgtgc cgaagcgcgc cagcacctgc tcgctgccgt
     3661 ggccgtgcac gacgacgatg cgcgccgcac cgagccgctg cgcgctggcg atgacatgcc
     3721 cgagcatcgg ccggccgccg accgcgtgca ggaccttggg caggtccgaa accatgcgct
     3781 tgcccttgcc ggcggcaagg atgacgacat ggaggttcgc aggacttgcg ggactcgaca
     3841 ggctcatcgg cgggggactc cggcggcggc acggccgcaa aagcaaacgc cgcggacagg
     3901 gccgcggcgt gggtcgatac gggttgccgc ttgcaggcgg cgatcaggcc ttgcgcagct
     3961 tgcgcagctt ttcgatcgcc tgcagctggg ccacggcctc ggccagttcg gcctgggcgc
     4021 gggcgaggct gatctcgtcg ctgcgggcag cgaggatttc ctcggcgcgg cgcttggctt
     4081 cgatcgcctt cgcctcgtcg aggtcggcag cacgctcggc ggtgtccgca agcacggtca
     4141 ccgtgtgcgg ctgcacttcg agcagaccgc cggagacgta gaacacctgc tcctcgccga
     4201 actgcagctt cacgcgcacc tgaccgggct tgagccgggc cagcatctgg ctgtggcgcg
     4261 gcaggatgcc gagttcgcct tcctcggccg tcgcgaccag catctccgcg gtgccggaga
     4321 agatctgctt ctcggcactc acgatgtcca catgcagcgt catcgccatg acgtcgttac
     4381 ctcgtcggat cgtcgggacg cgcgccggag tgaggatgat tcactccggc acgccgggtc
     4441 gccttacagc ttggcggcct tctcgacggc ttcgtcgatg gaaccgacca tgtagaaggc
     4501 ctgctccggc aggtggtcgt actcgccggc gatgatgccc ttgaagccgc ggatcgagtc
     4561 cttcagcgac acgtacttgc ccggggagcc ggtgaagact tccgcgacga agaacggctg
     4621 cgacaggaag cgctggatct tgcgggcacg ggcgacgacc tggcggtctt cttccgacag
     4681 ctcgtccatg ccgaggatcg cgatgatgtc cttcagctcc ttgtagcgct gcagcacacc
     4741 ctgcacggcg cgggcggtgc tgtagtgctc ctcgccgatg atcagcggat cgagctgacg
     4801 cgaggtcgag tcgagcgggt cgacggccgg gtagatgccg agctcggcga tctggcgcga
     4861 cagcacgacg gtcgcgtcaa ggtgcgcgaa ggtcgtcgcc ggcgacgggt cggtcaggtc
     4921 gtccgcaggc acgtacacgg cctggatcga cgtgatcgag cccttcttgg tcgaggtgat
     4981 gcgctcctgc agcacaccca tttcctcggc cagcgtcggc tggtaaccca ccgccgacgg
     5041 catgcgcccg agcagcgccg acacttcggt accggcgagc gtgtagcggt agatgttgtc
     5101 gacgaacagc agcacgtcac ggccttcgtc acggaagtac tcggccatgg tcaggccggt
     5161 cagcgcgacg cgcagacggt tgcccggcgg ctcgttcatc tggccgtaaa cgagcgcgac
     5221 cttgtcgagc acgttcgagt ccttcatctc gtggtagaag tcgttgccct cacgggtacg
     5281 ctcgcccacg cccgcgaaca cggaataacc ggagtgctcg atcgcgatgt tgcggatcag
     5341 ctccatcatg ttcacggtct tgccgacgcc ggcgccgccg aacaggccga ccttgccgcc
     5401 cttcgcgaac gggcagagca ggtcgatgac cttgatgccg gtttcgagca ggtcggtgga
     5461 accggactgt tcctcgtagg ccggagcctt gcggtggatc gcccaggtcg tctcggcgcc
     5521 gaccggaccc tgatggtcga tcggggtgcc gagcacgttc atgatgcggc ccagcgtcgc
     5581 cttgccgacc ggcacgttga tcgccgcacc ggtgttgttg gcgaccatgc cgcggcgcag
     5641 gccgtcggag gtaccgagcg cgatggcgcg cacgatgccg tcgccgagct gctgctgcac
     5701 ttccagcgtc agatccgcac cgtcgatctt gagcgcgtca tagatcttcg gcaccgcacc
     5761 gcgcgggaac tcgacgtcca cgacggcgcc gatgacttgt acgatctttc cagaactcat
     5821 tagagggttc cccttaagac tctgctgatt ctcgaagact ctcgaaacct gcgatggcag
     5881 cccgatggat caaacggccg cggcgcccgc cacgatctcg gaaagctcct gcgtgattgc
     5941 cgcctgacgc gccttgttgt agacgagctg caattcgtcg atgaggctgc cggcgttgtc
     6001 cgaggccgac ttcatcgcga tcatgcgcgc cgccatttcg caggccacgt tctcgacgag
     6061 tccctgatac accagcgatt cgacgtagcg gaccagcagg gtgtccagca gcaccgtcgc
     6121 atcgggctcg tacaggtaat cccagcggtg cggagcatcg accttttcga cgcgcgccga
     6181 gaccggcacc agcagctcca cgctcggctt ctgcgtcatc gtgttcagga agacgttatg
     6241 cacgaggtag atgcggtcga tctcgccttc gcggaagcgg tcgaacatga ccttcacgct
     6301 gccgaccaga tcctcgatcc gcggcgtgtc acccaggtgc gacacggtgg cagcgacctt
     6361 cacgcccaga cggcggaaga agcccgcagc cttggtgccg accgtacaca gttcgctttc
     6421 cacccctttg gcctgccact cgcgcatcga ctgcacggtg gccttgaaca ggttggtgtt
     6481 caggccaccg cacagaccgc ggtccgtgga gatcacgatg taaccgaccc gcttcacgtc
     6541 gggccggtcc atcagggccg gggggcgata ctcggtgtgc gcatgggcca ggtgcgagat
     6601 gacggtgcgg atcttctccg cgtagggccg ggccgcatac atgcgttcct gagccttgcg
     6661 catcttgctc gccgcaacca tttccatggc acgagtgatc ttctgagtat tcttgatact
     6721 cttgatcttc gttcgaatct ctttggcacc ggccatgctg acacccgctt accagacgtg
     6781 gttggccttg aaatcttcca cgcccttcgc gaaggccgct tcgatttcgc cgttgtagtc
     6841 gccggtctcg ttgaccttct tcatcagctc gctctggttc gcgttcatgt acgcgatcag
     6901 cgccgattcg aagtcaccga tcttgttcag cggcacgtcg tccagatagc ccttgtcggc
     6961 cgcaaacagc gagatcgcca tttcgccgac ggacagcggc gaatactgct tctgcttcat
     7021 cagctcggtg acgcgctgac cgcgttcgag ctgcttgcgg gtcgcatcgt cgaggtccga
     7081 ggcgaactgc gcgaaggccg cgagttcgcg gtactgcgcg agcgccagac gcacgccgcc
     7141 gccgagcttc ttgaccgcct tggtctgcgc cgcaccgccg acgcgcgaca ccgacagacc
     7201 ggcgttgatc gcgggccgga t
//
LOCUS       DAOQIE010000205         7201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5466_length_7201_cov_7.715365, whole genome shotgun sequence.
ACCESSION   DAOQIE010000205 DAOQIE010000000
VERSION     DAOQIE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5466_length_7201_cov_7.715365"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            724..2160
                     /gene="glgC"
                     /locus_tag="PK144_12655"
     CDS             724..2160
                     /gene="glgC"
                     /locus_tag="PK144_12655"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNF66620.1"
                     /translation="MHPTKPVMPNVESRFVSRLTRETLALVMAGGRGTRLRNLTQHRA
                     KPAVPFGGKFRIIDFTLSNCINSGIRRIGVLTQYKSHALIRHLERGWSFLRGQFGEFI
                     ELLPAEQNSDLSSWYVGTADAVYQNIEFIRRHQPRYVLVLAGDHVYNMDYGPMLAHHA
                     ASGADITIGCIEVPLSEAVDFGVMEIDESTRVHGFIEKSPNPKPMPGRPDTALASMGI
                     YVFEARYLLDVLKRDAGMRNSGHDFGHDVIPEAIRAGRVHAFPFRDLFDPDRQGYWRD
                     VGTVDAFWRANIELCGAEPVLNLDDERWPVWTGEEQSAPARFSSVPDGPRSVIEDALC
                     CGGCTVTGATVERSVLFTGARVEAGSRIEESLLLPDVRVGRNCRIRRAVIDEGCVVPD
                     GLVIGEDAASDQRLFHRSSEGIVLVTQEMLDNLQARGAGAAMPLPAVPPETAPVRAPA
                     PRTPLTVRTPLPGPHTGRNGRRPDLALN"
     gene            2424..4829
                     /gene="glgX"
                     /locus_tag="PK144_12660"
     CDS             2424..4829
                     /gene="glgX"
                     /locus_tag="PK144_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNF66621.1"
                     /translation="MPVADTLLPSSATALGALWDGRGAHFAVFAEDAEAVELCLFDAR
                     GEERRLQLPDCTNGIWRGYLPRVQPGQRYGYRVYGPYDPQRGHRFNPHKLLIDPYARQ
                     LAGALRWNKALYGYRMGGAKADLVPDRTDSARYLPKAVLVDASFDWGNDRAPRTPWSD
                     TVIYELHVKGFTQRFPGLGDTERGSFGALGDPAVIDYLRRLGVTAVELLPIHAFARDH
                     HLLDQGLTNYWGYNTLSYFAPEPAYLSDGTLGQIKWAVRELHAAGIEVILDVVYNHTC
                     EGSELGPTLSWRGFGNASYYRLLPDNARYHINDTGCGNTVNFSHPAVIRMVMDSLRYW
                     VQEFHVDGFRFDLGVTLGREVHGFDPGCGFFDALLQDPLLARVKLISEPWDIGPGGYQ
                     IGQHPAGMAEWNGRFRDDVRCYWRSDDGLRSALAARLQGSAELFDHHRRKPWASVNFI
                     TAHDGFTLRDLVSYNDKHNEANGEDNRDGGNDNASCNWGCEGPTDDVIVNDARLRVSR
                     SLLMTLFFSHGTPMLLAGDEFGQTQAGNNNVYCQDNELSWLDWERAATGEGRALRTFV
                     ERLIRLRRSWPLLQGLYFQHGHHESDAGLRDVFWFDERGVELIDSDWHNVAARLLGLR
                     RAARRADGRLDVLVLLHNADGAGHTFRLPPPLYDFRVLIDSAHPELDSDPVRDGRYVV
                     SPNTCVLLAGHQRREPLPDEVAVESAALAVAGAAPVDAISDPPDGPIDPAPLPPPEPA
                     PDPAPEPWPEPVPDPVPEPRPQPVPDPAPEPPPRPWPDPAPEPQPEPLPGFDDPGPLP
                     VPA"
     gene            4826..6652
                     /gene="treZ"
                     /locus_tag="PK144_12665"
     CDS             4826..6652
                     /gene="treZ"
                     /locus_tag="PK144_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNF66622.1"
                     /translation="MNAGVQRPVACGCPARADHDFGARVLDDGQVQFRLWAPGCTQVA
                     LELELDGRPPLAMTALDGGWFEARTDAAPGARYRYRVAADLAVPDPASRLQAGDVHEA
                     SVVVGPGDYVWQYPDWRGRPWPQMVILEVHAGVCGGFDGVRAQLPRWAEAGITAIELM
                     PIADFPGRRNWGYDGVLPYAPDTAYGTPAQLRALIDAAHGLGLCVYLDVVYNHFGPEG
                     NYLHAYAPQFFVADAQSPWGATIDFSQPAVRAFFTGNALYWLLDYRFDGLRFDAVHAI
                     PDPGWLDEMAAAVRAATPGRHVHLMLENEHNTASHLDGDFDAQWNDDAHNALHVLLTG
                     EREGYYANYAGRAGELFVRCLGEGFGWQGEPSPGHDGRPRGTPSAHLPPSAFVFFLQN
                     HDQIGNRALGERLLALCAQAGRLEAWRAALAVQLLCPQVPLLFMGDELGSTTPFLFFT
                     DFHDELAEAVREGRRREFAQFAAFADPERRAAIPDPNAGTTFESSRPQPGDPEDAARV
                     AMLLRLRNTLLAPRLAGTRALGSAALGPAALRARWRLGDGTMLTIYTNLADMPVELDF
                     VPPAGSLLYESRDGAAAALARDDLAAAATTVWLSAGTVPDSP"
     gene            6665..>7201
                     /locus_tag="PK144_12670"
     CDS             6665..>7201
                     /locus_tag="PK144_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNF66623.1"
                     /translation="MPHAPADLAALAQTAGLAAEWTDYRGIRHAIAPATQQQVLEALG
                     IDCTGAAAIADSRARLLAEAAAPPALAVLRSAEPWTLPAAFGTQPGAAVELVDEAGEC
                     VGTRLIADAAGRAALPGLLLDAGYYTLRLDGHAQALALAPAGAPGFARLAGRERGFGF
                     AAQLYGLRRGADGGVGDFS"
BASE COUNT         1022 a         2644 c         2484 g         1051 t
ORIGIN      
        1 gtgtggcggg cgtacggatc ggcggcagcg acggtcacgg caggtctggg gctcgggcgg
       61 acgcgggacc gcgcatggtg agacgcgcgc gccacggcag caagcacggt gcgggctttg
      121 cgcgatgtgc aagtgcagcg acagagcgga tcgcgcggcg cagcaggctg aacgtttggc
      181 aatgaaagtg aactgtcgtt gcgggaacgc tttgcccttg cgctcatcga aagtgctgtt
      241 cgtcgcttcc ggcgtacggg ccgtgattgc cggaccggca cggcccgcca ccgcaaacgc
      301 acggcgtgca gcgtccccac ccgatgcagg gccacgggcc gaaatgtccg ggcgagcgtc
      361 gctgcgcgcc cgcggcaggc gaacgggccg caggcgcatt ggcagcggga cgggcgggat
      421 tgccgatggc cgcgtcaggc gcgcaccgcg gttcaggtaa tgattccggt ggttccggcg
      481 taatggtttc acccgatggt ttcacccgca gtttcagccc attcaaggca gtaccggatc
      541 aagggtcggc agacggaacg ggccggcatg cgggtggccc gcaatcagca gggaaaccgg
      601 caggcgtctg attcccgatg ctgccggtcc ggaacttccg gtgcgggctg ccattccaga
      661 tgcgatgaca atgggccgtg cgccagccgg cgaacggcgg ttcgatccga ggagattcag
      721 ccgatgcacc cgaccaagcc cgtgatgccg aacgtcgagt cccgtttcgt cagtcgtctg
      781 acccgtgaaa ccctggcgct ggtcatggcc ggcgggcgcg gcacgcgcct gcgcaacctg
      841 acccagcacc gcgccaagcc ggccgtgccc ttcggcggca agttccggat catcgacttc
      901 acgctgtcga actgcatcaa cagcggcatc cgccgcatcg gcgtgctgac gcagtacaag
      961 agtcacgcgc tgatccgcca tctggagcgc ggctggagct tcctgcgcgg ccagttcggc
     1021 gaattcatcg aactgctgcc ggccgaacag aactccgatc tcagctcctg gtatgtcggc
     1081 accgccgacg ccgtttacca gaacatcgaa ttcatccgcc gccaccagcc gcgttacgtg
     1141 ctggtgctgg ccggggatca cgtctacaac atggattacg ggccgatgct cgcgcatcac
     1201 gcggccagcg gtgccgacat cacgatcggc tgcatcgagg tgccgctgtc cgaggccgtc
     1261 gatttcgggg tcatggaaat cgacgaaagc acgcgcgtac acggcttcat cgaaaaatcc
     1321 ccgaatccga aacccatgcc cggccgtccg gacaccgcgc tggcgagcat gggcatttac
     1381 gtgttcgaag cgcgttatct gctcgatgta ttgaagcgcg acgccggcat gcgtaattcc
     1441 gggcatgatt tcggccatga cgtgattccg gaagcgatcc gcgccgggcg cgtccacgcc
     1501 ttcccgttcc gcgacctgtt cgatcccgac aggcagggct actggcgcga tgtcggtacc
     1561 gtcgatgcct tctggcgcgc caacatcgag ctctgcggcg ccgagccggt gctgaacctc
     1621 gatgacgagc gctggccggt gtggaccggc gaggaacagt cggcgccggc gcgcttctcg
     1681 tcggtgccgg acggcccgcg cagcgtgatc gaggatgcgc tctgctgcgg cggctgcacg
     1741 gtgaccggcg ccacggtcga gcgctcggtg ctgttcaccg gcgcccgcgt cgaggccggc
     1801 agccgcatcg aggaatccct gctgctgccc gacgtgcgcg tcgggcgcaa ctgccgcatc
     1861 cgccgcgccg tcatcgacga gggctgcgtc gtgcccgacg gcctcgtgat cggcgaggac
     1921 gccgccagcg accagcgcct gttccaccgc agcagcgaag gcatcgtgct ggtcacgcag
     1981 gagatgctcg acaacctgca ggcgcgcggg gccggggccg ccatgccgct gccggccgtg
     2041 ccgccggaga ccgcacccgt ccgggcaccg gccccgcgca cgccgctgac ggtgcgcacg
     2101 ccgctgcccg gcccgcacac cggccgcaac ggccggcggc cggacctcgc gctgaactga
     2161 ggacggcctg ccgcactccg ttaatcctgc cgggcaaaag ccgcttttgg tttttcgggt
     2221 tccgccggcg cgcccggtgg atcgtgtgac cccccgcaca cggtccgccg ggcgtgtccg
     2281 gtgcctgtcc gggctggcag ccggcggcag ccgcgaaggc cggagccgcc gcttggggaa
     2341 gtccggtcac cgggcggcag aacccggtcc gacctgccac cgtgtaccgg ccctgccgac
     2401 gatccgccct gcacgaggac accatgcctg tcgccgacac cttgctgccg agttccgcca
     2461 ccgccctcgg cgccctctgg gatgggcgcg gtgcgcattt cgccgtgttc gccgaggatg
     2521 ccgaggccgt cgagctgtgc ctgttcgatg cccgcggcga ggaacgccgg ctgcagctgc
     2581 cggactgcac gaacggcatc tggcgcggct acctgccgcg cgtgcagccc ggccagcgct
     2641 acggctaccg cgtgtacggg ccgtacgacc cgcagcgcgg tcaccgcttc aatccgcaca
     2701 agctgctgat cgacccgtat gcgcgccagc tcgccggggc gctgcgctgg aacaaggcct
     2761 tgtacggcta ccgcatgggc ggtgcgaagg ccgatctggt gccggatcgc accgacagcg
     2821 cccgctacct gccgaaggcg gtgctggtcg atgcctcgtt cgactggggc aacgaccggg
     2881 cgccgcgcac gccgtggtcg gacacggtga tctacgagct gcacgtcaag ggcttcacgc
     2941 agcgctttcc cggcctgggc gacaccgagc gcggcagctt cggcgcgctc ggcgatccgg
     3001 cggtcatcga ctacctgcgc cggctcggcg tcaccgccgt cgagctgctg ccgatccatg
     3061 cctttgcccg cgatcaccac ctgctcgacc agggcctgac caactactgg ggctacaaca
     3121 cgctgtcgta cttcgcgccg gaaccggcct acctgtccga cggcacgctc ggccagatca
     3181 agtgggcggt ccgcgagctg cacgccgccg gcatcgaggt gatcctcgat gtcgtctaca
     3241 accacacctg cgagggcagc gaactcggtc cgacgctgag ctggcgcggc ttcggcaacg
     3301 ccagctacta ccggctgctg ccggacaacg cccgctacca catcaacgac accggctgcg
     3361 gcaacaccgt caacttctcg catccggcgg tgatccgcat ggtcatggat tcgctgcgct
     3421 actgggtgca ggaattccac gtcgacggct tccgcttcga cctcggcgtc acgctcgggc
     3481 gcgaggtgca cggcttcgac cccggctgcg gcttcttcga tgcgctgctg caggacccgc
     3541 tgctcgcccg cgtcaagctg atctccgaac cctgggacat cggccccggc ggctatcaga
     3601 tcggccagca cccggccggc atggccgagt ggaacggacg cttccgcgac gacgtgcgct
     3661 gctactggcg cagcgacgac ggcctgcgca gcgcgctggc cgcgcgcctg cagggctcgg
     3721 ccgaactctt tgaccaccac cggcgcaagc cctgggcgag cgtgaacttc atcaccgcac
     3781 atgacggttt cacgctgcgc gacctcgtca gttacaacga caagcacaac gaggccaacg
     3841 gcgaggacaa ccgcgacggc ggcaacgaca acgccagctg caactggggc tgcgagggac
     3901 cgaccgacga cgtgatcgtc aacgacgcgc gcctgcgcgt cagccgcagc ctgctgatga
     3961 cgctgttctt cagccacggc acgccgatgc tgctcgccgg cgacgagttc gggcagacgc
     4021 aggccggcaa caacaacgtg tactgccagg acaacgagct gtcgtggctc gactgggagc
     4081 gcgccgcaac cggcgagggc agggcgctgc gcaccttcgt cgagcgcctg atccggctgc
     4141 ggcgcagctg gccgctgctg caggggctgt atttccagca cggtcaccac gagtccgacg
     4201 cgggcctgcg cgacgtgttc tggttcgacg agcgcggcgt cgagctgatc gacagcgact
     4261 ggcacaacgt cgccgcgcgc ctgctcggcc tgcgccgtgc cgcccgccgt gccgatggcc
     4321 ggctcgacgt gctggtgctg ctgcacaacg ccgacggggc cgggcacacg ttccggctgc
     4381 cgccgccgct gtatgacttc cgcgtgctga tcgacagcgc ccaccccgaa ctcgacagcg
     4441 atccggtgcg ggacggccgc tacgtggtct cgccgaacac ctgcgtgctg ctcgccggcc
     4501 accagcgccg ggagccgctg cccgacgagg tggcggtcga atcggccgcg ctcgcggttg
     4561 ccggcgcagc gccggtcgat gcgatcagcg atcccccgga cggcccgatc gatccggcgc
     4621 cgctgccgcc gcccgagccg gcgccggacc cggcgcccga gccctggccc gagcccgtac
     4681 ccgatccggt gcccgaaccg cgtccgcagc cggtccccga cccggctccg gagcccccgc
     4741 cgcgcccgtg gcccgatccg gcgcccgagc cgcaacccga accgctgccg ggcttcgatg
     4801 atcccgggcc gctgccggtg ccggcatgaa cgccggcgtg cagcgccccg tcgcctgcgg
     4861 ctgtcctgcg cgtgccgatc acgacttcgg cgcgcgcgtg ctcgatgacg ggcaggtgca
     4921 gttccggctg tgggcaccgg gctgcacgca ggtcgcgctc gagcttgaac tcgacggccg
     4981 gccgccgctg gcgatgacgg cactcgatgg cggctggttc gaggccagga ccgacgccgc
     5041 tcccggcgcg cgctaccgct accgcgtcgc tgcggacctg gccgtgccgg acccggcctc
     5101 gcggctgcag gccggcgacg tgcatgaggc cagcgtcgtc gtcgggcccg gtgactacgt
     5161 ctggcagtat ccggactggc gcggccggcc gtggccgcag atggtcatcc tcgaagtcca
     5221 cgccggcgtg tgcggcggct tcgacggcgt gcgcgcgcag ctgccgcgct gggccgaagc
     5281 cggcatcacg gcgatcgagc tgatgccgat cgccgatttc ccgggccggc gcaactgggg
     5341 ctacgacggc gtgctgccct acgcgccgga caccgcctac ggcacgcccg cgcagctgcg
     5401 cgcgctgatc gacgccgcgc acggactcgg gctctgcgtc tacctcgatg tcgtctacaa
     5461 ccacttcggc cccgagggca attacctgca cgcgtatgcg ccgcagttct tcgtcgccga
     5521 tgcgcagtcg ccgtggggcg cgaccatcga cttctcgcaa ccggcggtgc gggccttctt
     5581 caccggcaac gcgctgtact ggctgctcga ctaccgcttc gacggcctgc gcttcgacgc
     5641 cgtgcacgcg atcccggacc ccggctggct cgatgagatg gccgctgccg tgcgcgccgc
     5701 gacccccggc cggcacgtgc acctgatgct cgaaaacgag cacaacacgg cttcgcacct
     5761 cgacggcgac ttcgacgcgc agtggaacga cgacgcgcac aacgcgctgc acgtgctgtt
     5821 gaccggcgag cgcgagggct attacgcgaa ctacgccggg cgtgccggcg agctgttcgt
     5881 gcgctgcctc ggcgaaggct tcggctggca gggcgagccc tcgccgggcc acgacggccg
     5941 gccgcgcggc acgccgagcg cgcacctgcc gccgtcggcc ttcgtgttct tcctgcagaa
     6001 ccacgaccag atcggcaacc gcgcgctcgg cgagcggctg ctggcgctct gcgcgcaggc
     6061 cggccggctc gaagcctggc gcgcggcgct ggccgtgcag ctgctctgcc cgcaggtgcc
     6121 gctgctgttc atgggcgacg aactcggctc gaccacgccg ttcctgttct tcaccgactt
     6181 ccacgacgaa ctcgccgagg ccgtgcgcga gggccgccgg cgcgagttcg cgcagttcgc
     6241 ggccttcgcc gatcccgaac gccgcgccgc gatcccggac ccgaacgccg ggacgacgtt
     6301 cgagtcctcg cgcccgcagc ccggcgatcc cgaggatgct gcccgggtcg cgatgctgct
     6361 gcgcctgcgc aacaccttgc tcgcgccgcg gctggccggc acgcgggcgc tcggcagcgc
     6421 agcgctcgga ccggcggcgc tgcgtgcccg ctggcgcctc ggggacggta cgatgctgac
     6481 gatctacacg aatctcgccg acatgccggt cgagctcgac ttcgtgccgc cggccggctc
     6541 gctgctgtac gagagccggg acggcgccgc cgcggcgctg gcgcgtgacg atctggccgc
     6601 tgctgccacg acggtctggc tgtccgcagg cacggtcccc gattccccct gacggaagtc
     6661 caagatgccc cacgccccgg ccgatcttgc cgcacttgcc cagaccgccg gactggcggc
     6721 cgaatggacc gattaccgcg gcatccgcca cgccatcgcc cccgccacgc agcagcaggt
     6781 gctcgaagcc ctcggcatcg actgcacggg tgcggccgcg atcgccgaca gccgcgcgcg
     6841 gctgctggcc gaggccgcag cgccgccggc gctcgcggtg ctgcgcagcg ccgagccctg
     6901 gacgctgccg gcggccttcg gcacgcagcc cggcgcggcg gtcgaactcg tcgatgaggc
     6961 cggcgagtgc gtcgggacgc ggctcatcgc cgatgccgcc gggcgcgcgg cgctgccggg
     7021 gctgctgctc gatgccggct actacacgct gaggctcgac gggcacgcgc aggcgctggc
     7081 gctggccccg gcgggcgcac cgggctttgc ccggctcgcc gggcgcgagc gcggcttcgg
     7141 cttcgcggcc cagctctacg gcctgcgccg cggcgcagac ggcggcgtcg gcgatttctc
     7201 g
//
LOCUS       DAOQIE010000206         7125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5544_length_7125_cov_8.239745, whole genome shotgun sequence.
ACCESSION   DAOQIE010000206 DAOQIE010000000
VERSION     DAOQIE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_7125_cov_8.239745"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="gcvA"
                     /locus_tag="PK144_12675"
     CDS             complement(<1..889)
                     /gene="gcvA"
                     /locus_tag="PK144_12675"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HNF66624.1"
                     /translation="MAYRLPPLNALRAFEAAARHLSFSKAAEELFVTPAAISHQIKSL
                     EEFLGVQLFRRMNRALALTDAAQACLPRLRQGFDALSEAVDLVRAQEDSGLLTVSLTP
                     SFAVKWLVPRLDRFSALHPNIEMRIAASSRQLDLRGGGQRSTTDLVDFRREDVDLAIR
                     FGSGDYPDFHVTKLFSVHATPLCSPALLKRNPLNEPADLAQHVLLHDETVYGPEYTPE
                     REPMWRVWLRAAGVADRVDARRGPHFSSASLTIEAALDGQGVALGLPVLALADIQTGR
                     LVAPFGRQVPIEFAYWIVCP"
     gene            1061..1363
                     /locus_tag="PK144_12680"
     CDS             1061..1363
                     /locus_tag="PK144_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HNF66625.1"
                     /translation="MTRGDRSGLLQVVIMFSRFVVHPGAGHLVNAISGSGLMVPVRVD
                     IESPSRFVGALKLLRLWVMRARERRALVRLDEHALTDIGMSRADALSEAAKPFWRR"
     gene            complement(1733..3067)
                     /locus_tag="PK144_12685"
     CDS             complement(1733..3067)
                     /locus_tag="PK144_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF66626.1"
                     /translation="MNPTVLVVDDEPHVRLAAVQTLELEGITAEPLASAAAALARLSP
                     EFDGIVVSDVRMPGMDGLALQRAVAALDPELPVVLVTGHGDIAMAVQAIRDGAYDFLE
                     KPFRTEALLDIVRRGLDKRALTLENRRLRRELAAHREGDALLLGRSPGIARLRAAIDN
                     LADTGADVLIEGETGAGKEVVARLLHQRSHRRGQAFVALNCGAIPETIFESELFGHEA
                     GAFTGASGRRVGKLEHAHGGTVFLDEIESMPLALQVKVLRALQDRMIERLGSNRGVAL
                     DIRVVAATKVDLRVAAREGRFREDLYYRLNVVKLAIPPLRERREDIPLLFRHFVLKAA
                     ERYRREPPEVAGDTLAALMSEPWPGNVRELANAADRFVLGHLLGPAALAAGAPAQSLP
                     DQVAAFERIVIVQELERQDGNVVATADALGVPRKTLYDKLKKYGLRREDGFR"
     gene            complement(3064..4980)
                     /locus_tag="PK144_12690"
     CDS             complement(3064..4980)
                     /locus_tag="PK144_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66627.1"
                     /translation="MTAPQSQEPAAGVAGLPRGLRVTVIVGAVLVLAALVMLVAALLT
                     RQSALAELRAEAAPALQRSVSSLQGLLDTYQILPRVLAREPLILNLYATPTLSPATRE
                     TANEFLSAFNAAAGASETYVLDALGNTIAASNSESPVSFVGRNFAFRPYFHLAIAGWA
                     GLYFAHGTTSNRPGFYFSYPVKSSGGDAVGVAVVKIGQERLESAWSQSATPILVTDAA
                     GVVFITNREDFRYHALRPLPPVLAAELVGSRQYGDGPLAPLPVVGEREFDGVRLVSLH
                     EGRRSVEYLLAKAPVPNTDWEVSVLVPTATVEEQVGVAVLLAGSASAIATLFGLMLAQ
                     KRLRTHERLAYQARVAEALRESHDRLEAHVVERTRDLVEANQRLSREIQERERAEQIV
                     LKTQNELIQASKLAALGQLAAGIVHELNQPLAAIRAYAANARTFLERGRPEAASGNLD
                     LIGELTERMATLTAELKTFARRTSDEVADIDLRASVGRARALLDPRLRRDGIELHIEQ
                     PDTALTVRANAIRIEQVLVNLLKNAADALLEAATPVPRIGLRLQADPAARRARLVVTD
                     NGPGIPPEALGQLFDPFFTTKPVGEGLGLGLSISYGIVQNLGGTIRAENLAGGGACFT
                     VELPLPAPQPLESA"
     gene            complement(4977..5915)
                     /locus_tag="PK144_12695"
     CDS             complement(4977..5915)
                     /locus_tag="PK144_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNF66628.1"
                     /translation="MPASAALLPVLAALGPVFGLIALGTVLRRCGFPGSGFWQPAERL
                     SYYLLLPALLLRELARADFGALAVGGVVVALLATIALMSVLALRLRRWLGNDGPAFGA
                     SFQGVIRLNTYVGLATALPLYGAAGGALAALALAVMVPVVNLLSVLVITHALGQARPA
                     HLLRQLAGNPLILACAAGIALNISGLGLPPGSEGFTRILAQAALPLGLLAMGAALQLA
                     AVRGRAAAITVTVLLKLVLAPLLAAALARLCALPALESAVVVLFAALPSAPSAYILAR
                     QLGADAPLAAVVITVETLAAMLTLPLVVLLCAGPHS"
     gene            6164..>7125
                     /locus_tag="PK144_12700"
     CDS             6164..>7125
                     /locus_tag="PK144_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66629.1"
                     /translation="MAITYRLTTYGFQEGRPRGPARALMTGLLEGEPEDIERLADRVL
                     CGERLALAGGLDKADGERLRKALAGVGIEAKLYPESLSLLPVELEAPQAHTYRCPACH
                     HEQEVDPLAPDTCKSCGVIGSKFDEVREIKDVLDAERRKLEARISEAEAAEIEQQRQR
                     EHERLREIGRKRAEQQLGITRMTKLRALLRSPVFAPLAGGVAVAVLAGVGIWQYRVHQ
                     SPAPAAAQSSVAQIPGAVTPGAAPVQAVAADSSKRPQGAPPAEIEQALAAAEAGTGGG
                     RPGLTPQQAVMAVNQLASKLPGGGEAGPAAAAGFTAVPVAVPEAV"
BASE COUNT         1041 a         2511 c         2632 g          941 t
ORIGIN      
        1 tcgggcagac gatccagtac gcgaactcga tcggcacctg ccggccgaac ggggcgacca
       61 gccgcccggt ctggatgtcg gccagtgcca gcaccggcag gccgagcgcg acgccctgac
      121 cgtcgagtgc ggcctcgatg gtcagcgagg cgctgctgaa gtgcgggccg cgccgggcat
      181 cgacccggtc ggcgacgccg gccgcgcgca gccacacgcg ccacatcggt tcgcgctccg
      241 gcgtgtattc ggggccgtag acggtctcgt cgtgcagcag cacgtgctgt gcgagatcgg
      301 ccggctcgtt cagcgggttg cgcttcagca gcgccgggct gcagagcggc gtcgcgtgca
      361 ccgagaacag cttggtgacg tggaaatccg ggtagtcgcc gctgccgaag cggatcgcga
      421 ggtccacgtc ctcgcgccgg aaatcgacca gatcagtcgt gctgcgctga ccgccgccgc
      481 gcaggtcgag ctggcggctg ctggcggcga tgcgcatctc gatgttcggg tgcagcgccg
      541 agaagcggtc gagccgcggc accagccact tgaccgcgaa cgatggcgtc aggctgacgg
      601 tcagcaggcc gctgtcttcc tgcgcgcgca cgagatcgac ggcctcggac agcgcatcga
      661 agccctggcg cagccgcggc aggcaggcct gcgcggcgtc ggtcagcgcc agcgcgcggt
      721 tcatgcgccg gaacagctgc acgccgagga actcctccag cgacttgatc tggtggctga
      781 tcgccgccgg cgtcacgaac agttcctcgg cggccttgct gaaactcaga tggcgggcgg
      841 cggcttcgaa ggcgcgcagg gcgttcaggg gcggcaggcg ataggccatc ttcgttaaac
      901 ccggatttgt gcgagggtga gattttgtcg tttgcaggcg ccgcgggtgc ggaccagcat
      961 tgcaccaaca aagagcggta ctgtttcaaa ttggtgcaaa tcgttacaag tcattggttg
     1021 gtcgtcgcgt cacagggatg gtgtgaaccg aaacagtgca ttgacgcgag gcgaccgatc
     1081 ggggctgttg caggtggtca tcatgttcag tcgtttcgtc gttcatccgg gtgccggtca
     1141 tctcgtcaac gccatcagcg gcagcggcct gatggtaccg gtgcgcgtcg acatcgaatc
     1201 cccgtcgcgg ttcgtcggcg cgctgaaact gctgcggctg tgggtgatgc gcgccaggga
     1261 gcgccgcgcc ctggtccggc tcgacgagca cgcactcacc gacatcggca tgagccgcgc
     1321 cgatgcgctg agcgaggccg ccaagccgtt ctggcgtcgc tgaggcgcgt ccgggcgagc
     1381 ggcagcgggc ggtcgtttcc cggcgccgac ccggccggag cgcgccgcgc tcacggcggg
     1441 cgcgcctgac ggggccgtca cggccggtca tgcctgaggt tttccggacg ggggtgtccg
     1501 gacgctggta cacgggtcat ccggggggag tggcccgtgt gccggcactc catcgccggg
     1561 gtgaacatac atagcacgat gcgtgccggg ctgagcggac tgccggtcat ccagggctgg
     1621 ccgggacccg cgccggtgtc tgccgaagcc tgcgtgcctt gccggtgtgc aggcttcgcc
     1681 gcccggcacc ggaacggcca cgcgccggcc gcggcctgaa gcggcacggg cttcagcgga
     1741 aaccgtcctc gcggcgcagg ccgtacttct tcagcttgtc gtacagcgtc ttgcgcggca
     1801 cgccgagcgc atcggcggtg gcgacgacgt tgccgtcctg gcgttcgagc tcctgcacga
     1861 tgacgatgcg ctcgaaggcc gcgacctgat cgggcagcga ctgcgccggt gcgccggcgg
     1921 cgagcgcggc cggaccgagc aggtgaccga gcacgaagcg gtcggcggcg ttggcgagtt
     1981 cgcggacgtt gccgggccac ggctcgctca tcagcgccgc cagcgtgtcg ccggcgacct
     2041 cgggcggctc gcgccggtag cgctcggcgg ccttcagcac gaaatgccgg aacagcagcg
     2101 ggatgtcctc gcgccgctcg cgcagcggcg ggatcgccag cttgacgacg ttgagccggt
     2161 aatacaggtc ctcgcggaac ctgccctcgc gcgccgcgac gcgcaggtcg accttggtcg
     2221 cggcgacgac gcggatgtcg agcgcgacgc cgcggttcga gccgagccgc tcgatcatgc
     2281 ggtcctgcag cgcgcgcagc accttgacct gcagcgccag cggcatcgat tcgatctcgt
     2341 cgaggaacac cgtgccgccg tgcgcgtgtt cgagcttgcc gacgcgccgg ccgctggcgc
     2401 cggtgaaggc gccggcctcg tggccgaaca gctcggactc gaagatggtc tcggggatcg
     2461 cgccgcagtt gagcgcgacg aaggcctgcc cgcgccggtg gctgcgctgg tgcagcaggc
     2521 gcgcgaccac ctccttgccg gcgccggtct cgccctcgat cagcacgtcg gcgccggtat
     2581 cggcgaggtt gtcgatcgcc gcgcgcaggc gtgcgatgcc ggggctgcgc ccgagcagca
     2641 gcgcgtcgcc ctcgcggtgc gcggccagct cgcgccgcag ccggcggttc tccagcgtca
     2701 gcgcgcgctt gtcgaggccg cggcgcacga tgtcgagcag cgcctcggtg cggaaaggtt
     2761 tttccaggaa gtcataggca ccgtcgcgga tcgcctgcac cgccatcgcg atgtcaccgt
     2821 ggccggtgac cagcaccacc ggcagctccg gatcgagcgc ggcgacggcg cgctgcagcg
     2881 ccagcccgtc catgcccggc atgcgcacgt ccgagaccac gatgccgtcg aactccggcg
     2941 acagccgcgc cagcgccgcg gcggccgacg cgagcggttc ggcggtgatg ccttcgagtt
     3001 ccagcgtctg caccgcggcc aggcgcacgt gcggttcgtc atcgacgacg aggacggtcg
     3061 ggttcatgcg gactccagcg gctgcggggc cggcagcggc agttcgacgg tgaagcaggc
     3121 gccgccgccg gcgaggtttt cggcgcggat cgtgccgccg aggttctgga cgatgccgta
     3181 gctgatcgac agcccgagcc cgaggccctc gccgaccggc ttggtcgtga agaaggggtc
     3241 gaacagctgc ccgagcgctt cgggcgggat gccggggccg ttgtcggtga cgacgaggcg
     3301 tgcgcgccgc gcggccgggt cggcctgcag gcgcagcccg atgcgcggaa ccggcgttgc
     3361 ggcctccagc agcgcgtcgg cggcgttctt cagcaggttc acgagcacct gctcgatgcg
     3421 gatggcgttg gcgcgcacgg tcagcgccgt gtcgggctgt tcgatgtgca gttcgatgcc
     3481 gtcgcggcgc agccgcgggt cgagcagcgc acgcgcccgc ccgaccgagg cgcgcaggtc
     3541 gatgtcggcg acctcatccg aggtgcgccg cgcgaaggtc ttcagctccg cggtcagcgt
     3601 cgccatgcgc tcggtcagct cgccgatcag gtcgaggttg ccgctcgccg cctcgggccg
     3661 cccgcgctcc agaaaggtgc gggcgttggc cgcgtaggcg cggatcgccg ccagcggctg
     3721 gttcagctcg tggacgatgc cggcggcgag ctggccgagc gcggcgagct tgctcgcctg
     3781 gatcagctcg ttctgcgtct tcagcacgat ctgctcggcg cgctcgcgtt cctggatctc
     3841 gcgcgacagc cgctggttgg cctcgaccag atcgcgcgtg cgctcgacga cgtgcgcctc
     3901 cagccggtcg tgcgactcgc gcagcgcctc ggcgacgcgc gcctgatagg cgagccgctc
     3961 gtgcgtgcgc aggcgcttct gcgccagcat cagcccgaac agcgtcgcga tcgcgctcgc
     4021 cgagccggcc agcagcaccg cgacgccgac ctgctcctcg accgtcgccg tcggcaccag
     4081 caccgagacc tcccagtcgg tgttcggcac cggcgccttc gccagcaggt attcgacgct
     4141 gcgccggccc tcatgcaggc tgaccagccg cacgccgtcg aactcgcgct cgccgaccac
     4201 cggcagcggc gccagcggcc cgtcgccgta ctggcgcgag ccgaccagct cggcggccag
     4261 caccggcggc agcggccgca gcgcgtgata gcggaagtcc tcgcggttgg tgatgaacac
     4321 cacgccggcg gcatcggtga ccaggatcgg cgtggcgctc tgcgaccagg ccgattccag
     4381 gcgctcctgg ccgatcttga ccacggcgac cccgaccgcg tcgccgccgc tcgacttgac
     4441 cggatacgag aaatagaacc cgggccggtt cgaggtcgtg ccgtgcgcga agtacagccc
     4501 ggcccagccg gcgatcgcga gatgaaagta gggccggaac gcgaagttgc gcccgacgaa
     4561 ggacaccggc gattcggagt tgctggccgc gatcgtgttg ccgagcgcat cgagcacgta
     4621 ggtctcggag gccccggccg cggcgttgaa ggccgacagg aactcgttgg cggtttcgcg
     4681 cgtggccggt gacagcgtcg gggtcgcgta caggttcagg atcagcggct cgcgcgccag
     4741 cacgcgcggc aggatctggt aggtgtcgag caggccctgc aggctgctga ccgaacgctg
     4801 cagcgccggc gccgcctcgg cacgcagctc ggcgagggcg gactggcgcg tcagcagcgc
     4861 cgcgaccagc atcaccagcg ccgcgagcac cagcacggca ccgacgatca ccgtcacgcg
     4921 cagaccgcgc ggcaggccag cgacgccggc cgccggttcc tgcgactgcg gcgctgtcat
     4981 gaatgcggcc ctgcgcagag cagcacgacc agcggcagcg tcagcatcgc cgccagcgtc
     5041 tcgaccgtga tcaccaccgc cgcgagcggc gcatcggccc cgagctgacg cgcgaggatg
     5101 taggccgacg gcgccgaggg cagcgccgcg aacagcacga cgacggcgct ctccagcgcc
     5161 ggcagcgcgc agagcctcgc cagcgcggcg gcgagcagcg gcgccagcac gagcttcagc
     5221 agtaccgtca ccgtgatcgc cgcggcccgc ccgcgcacgg ccgcgagctg cagcgccgca
     5281 cccatcgcca gcagcccgag cggcagtgct gcctgcgcga ggatgcgcgt gaagccctcg
     5341 ctgcccggcg gcaggccgag gcccgagatg ttcagcgcga tgccggccgc gcaggcgagg
     5401 atcagcgggt tgccggcgag ctggcgcagc aggtgggccg gccgggcctg cccgagcgcg
     5461 tgcgtgatga cgaggacgct cagcaggttc acgaccggca ccatgaccgc cagggcgagc
     5521 gccgccagcg cgccgccggc ggccccgtac agcggcagcg ccgtcgccag cccgacgtag
     5581 gtgttgagcc ggatcacgcc ctggaagctc gcgccgaagg ccggcccgtc gttgcccagc
     5641 cagcggcgca gccgcagcgc cagcacgctc atgagcgcga tcgtcgcgag cagcgcgacg
     5701 accacgccgc cgacggccag cgcgccgaag tcggcgcgcg ccagctcgcg cagcagcagc
     5761 gccggcagca gcaggtagta gctcagccgc tcggccggct gccagaagcc gctgcccgga
     5821 aagccgcagc ggcgcagcac ggtgccgagc gcgatcagcc cgaacaccgg gccgagggcg
     5881 gcgaggacgg ggagcagggc ggcggaggcg ggcatcgatg cagcgtagcg aagcgcttgc
     5941 gcgatgtgaa atgcccgcga ggctccggcc ggctgcacta cgccggctgc cgcttgtccc
     6001 tgtcacaggc cggtgatatt gaagcctgcc ccgacttcgc cgtgcccggc cggggccaga
     6061 cccgggcatc ggcgaaacct cacaggaaac gacacggacc gtggcatcac gcagcggcac
     6121 cgcccggcgg atgcactgac gcagcccgag gagcaagcca gtcatggcga tcacctaccg
     6181 actcacgacc tacggcttcc aggaaggccg gccgcgcggc ccggcgcgcg cgctgatgac
     6241 cggcctgctg gaaggcgaac ccgaagacat cgagcggctc gccgaccgcg tgctctgcgg
     6301 cgagcgtctg gcgctggccg gcggtctcga caaggcggat ggagagcgcc tgcgcaaggc
     6361 gctcgccggc gtcggcatcg aggccaagct ctatcccgaa tccctgagcc tgctgccggt
     6421 cgagctcgaa gcaccgcagg cgcacaccta ccgctgcccc gcctgccacc acgagcagga
     6481 agtcgatccg ctcgcgcccg atacctgcaa gtcctgcggc gtgatcggca gcaagttcga
     6541 cgaggtgcgc gagatcaagg acgtgctcga cgccgagcgg cgcaagctgg aggcgcgcat
     6601 cagcgaggcc gaggccgccg aaatcgagca gcagcgccag cgcgagcacg agcgcctgcg
     6661 cgagatcggc cgcaagcgcg ccgagcagca gctcggcatc acccgcatga ccaagctgcg
     6721 ggcgctgctg cgcagtccgg tgtttgcgcc actcgccggc ggcgtggcgg tcgccgtgct
     6781 cgccggcgtc ggcatctggc agtaccgcgt gcatcagtcg ccggctccgg cggccgcgca
     6841 gtcgtccgtg gcacagattc cgggcgccgt gacgccgggt gctgccccgg tgcaggcggt
     6901 ggcagccgac agcagcaagc gtccgcaggg cgcaccgccg gccgagatcg agcaggcgct
     6961 cgccgcggcc gaggccggca ccggcggtgg ccggccgggc ctgacaccgc agcaggcggt
     7021 gatggcggtc aaccagctgg catcgaagct gccgggcggc ggtgaggccg gccccgcggc
     7081 ggcggccggc ttcacggcgg tgccggtggc cgtgccggag gccgt
//
LOCUS       DAOQIE010000207         7062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5619_length_7062_cov_7.906237, whole genome shotgun sequence.
ACCESSION   DAOQIE010000207 DAOQIE010000000
VERSION     DAOQIE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5619_length_7062_cov_7.906237"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..3766)
                     /gene="mfd"
                     /locus_tag="PK144_12705"
     CDS             complement(323..3766)
                     /gene="mfd"
                     /locus_tag="PK144_12705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNF66630.1"
                     /translation="MPPKSAASPATALPALPLPADALKPGRWSGLSEAAQALAIAATA
                     ARTQALLLVLAPDTQTALRLDAAIGWFAGTLDVPRLVFPDWETLPYDQFSPHQDLISQ
                     RLATLYRLPMQKRGVLIVPAPTLMQRLGPKSFLDAHALLLATGDRLDVDAFRTRLEAA
                     GYLCVAQVMEHGEFAVRGSIIDVFPMGAEAPLRIDLFDDEIESLRSFEPETQRSIAKL
                     DAIELLPAREFAMTRESITRFRTNFRATFEGDPQQSPIYREVSQGFAPPGIEYWLPLF
                     FEQTSTLFDFLPSNALVLRLPGWEAAMDGFIAQVQERYESRRHDRTRPLLAPAQLFLD
                     AGEVNRRVSRLLRVDLDSTGAPALPELYFAPRAERPAAALLDWLDAFPGRVLFAAESA
                     GRREVLAETLRAQNLLPVAVDGWPGFVGSEARLALTVAPLDQGFVLEDGTAAPLAVIT
                     ESLLHREWVRSTRRSSRAAREAEAIIRNLADLAIGAAVVHEDHGVGRYQGLVHMEAGG
                     IPAEYLVLEYANAEKLYVPVAMLDRVSRYTGGENPPLHRLGGEQWEKARRKAAEKAHD
                     AAAELLDVYARRAARPGHKFHGDEREMLAFAAAFPYEETPDQQLAIDAVLADLRTGKP
                     MDRVVCGDVGFGKTEVAMRAAFLAVQDGRQVAVLVPTTLLANQHYRNFLDRYAGWPVT
                     IESLSRFRSKKDTDAVLAGLASGGIDIVIGTHKLIQPEVRFKDLGLVIIDEEHRFGVR
                     QKEAFKALRSEVDILTLTATPIPRTLNFALAGLRELSIIATPPASRLAVKTFVNEYNA
                     ALIEEACLRELKRGGQVYFLHNEVDSIHRRAQELAQLVPGARVAVAHGQMGAGELEKV
                     MLDFYHQRCNLLVCTTIVESGIDVPSANTIIIERADKLGLAQLHQLRGRVGRSHHRAY
                     CYLIVPNRKSMTADAVKRIEAIESLEELGAGFMLANHDLEIRGAGELLGEEQSGQIHE
                     VGYTMYMDMLERAVKAIRSGRTPALDRPLDHGPEVSLGVPALIPEDYLPDVHARLMLY
                     KRIASCTDAAALQELQVEMIDRFGLLPAQVQNLFRITELKLAAGPVGIRKIEAGPEGG
                     RIVFGPEPNIDVSRLLVLIHKQPKTYRLDGQDKLRIQKPLADAEARAAEVRMLLGQIA
                     A"
     gene            4013..4672
                     /locus_tag="PK144_12710"
     CDS             4013..4672
                     /locus_tag="PK144_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66631.1"
                     /translation="MIRVMLVDDHQLIRIALGQILGGHADIEVVAEAADGEEALVRAR
                     AAKPDVILIDVDMPGMGGVEATRRLSALAHKPRVVAISVHDQMPYPQRMMEAGAVGYL
                     PKGGSADEVLAAVRAVARGQPYIAPVIAGKMFLTSMNRSGSAGNGAQVLDTLSAREMQ
                     VMLALVNGQNPQQIGEALKISAKTVCTHRYHIYEKLGVDSDVALTHLAYRYGLIEVSQ
                     S"
     gene            complement(4689..5027)
                     /locus_tag="PK144_12715"
     CDS             complement(4689..5027)
                     /locus_tag="PK144_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HNF66632.1"
                     /translation="MTTETESAAGRGHCRLFVTYSGVRLPLKLSGPIEDTAHRNTYFR
                     GWFDAGERVTEIEKLVYGEVEMRHRYAYHANGTLAQAEITDADGEIRVLHFDAQGAPL
                     AHDDDDDDDE"
     gene            5191..5727
                     /locus_tag="PK144_12720"
     CDS             5191..5727
                     /locus_tag="PK144_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66633.1"
                     /translation="MQTVTAQEDLRLRAALEVQRYRLGRSLRGCAAAALTGLLLALGG
                     LHEDRLPTPLALGFLVTLGGFASLGALLLQRRFARLARVVPRLRATPCLALLAVGGWF
                     QEPEYQADVRIPGLWHWHVDLETPLPDPHLEAGGDQPIDAIAWLEPRRHRLWALELRT
                     VVFWVRRCVRVEPATRRA"
     gene            complement(5752..>7062)
                     /locus_tag="PK144_12725"
     CDS             complement(5752..>7062)
                     /locus_tag="PK144_12725"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNF66634.1"
                     /translation="ITAQGRALLALGLHPRLGHLLLRGRALGLGALACDLAALLDERD
                     PLRGEAARSADLTLRLEALAAVRAGAREAARGFGADANACARLLEAARVLRERLDVPA
                     ADPRASIRSQDLALLVAAAYPDRIARRRDGSPDRFLLASGRGARLREGDALTGSDWLA
                     VAALDAGEREGRIFLAVALDLPTLETALAGHIAEVAEVRWDEREQAVVARRECRLGAL
                     LLDARALARPEPAALRRAMCAGIELLGLDALPWTDALRDWQKRVQSLRHWQPEAGWPE
                     VSDAALAATLGDWLQPYLDGCTRREHLARLDLAAILHARLDWPQTQRLATLAPTHLDV
                     PSGSRLRLEYAADGAAPVLAVKLQEMFGLADTPRIADGRIAVTLHLLSPARRPIQVTQ
                     DLRGFWERTYAEVRKELKGRYPKHPWPDDPWTAVPTARAKPRGR"
BASE COUNT          953 a         2438 c         2660 g         1011 t
ORIGIN      
        1 ccggggggag cggattgcgg gcgcggggca gccggctgcg ctgcggtggc agcgggcggg
       61 agggcgatgg cagccggggc tggccaggat cgagccgggt gcggtcgatg ccggcgcggt
      121 acgggcggtg ggcgaaccgg ttacggatgt cgcgggctgg cttgcggcag cggcgatggc
      181 cggtggagcg gtcgggcagc ggggcgcggg tgagggggcg tcgggacagg acgcaagaaa
      241 tccggccagg atgcagaaga tcaggatgcg cccgccccac ggctgcgggc ttgatttccg
      301 gcaccggctc cgcgccgccg cttcaggccg cgatctgccc gagcagcatg cgcacctcgg
      361 cggcccgcgc ctcggcgtcg gccagcggtt tctggatgcg cagcttgtcc tgaccatcga
      421 gccggtaggt tttcggctgc ttgtggatca gcaccagcag gcggctcacg tcgatgttcg
      481 gctccggccc gaacacgatg cgcccgccct ccgggccggc ctcgatcttg cggatgccga
      541 ccgggccggc ggcgagcttc agttcggtga tgcggaacag gttctgcacc tgcgccggca
      601 gcaggccgaa gcggtcgatc atctcgacct gcagctcctg cagcgccgcg gcatcggtgc
      661 agctggcgat gcgcttgtac agcatcagcc gcgcgtgcac gtcgggcagg tagtcctcgg
      721 ggatcagcgc cggcacgccg agactgacct cggggccgtg atcgagcggg cggtcgagcg
      781 ccggcgtgcg cccggaccgg atcgccttca ccgcgcgttc gagcatgtcc atgtacatcg
      841 tgtagccgac ctcgtggatc tggccgctct gctcctcgcc gagcagttcg ccggccccgc
      901 ggatctccag gtcgtggttg gcgagcatga agccggcgcc gagttcttcg agcgattcga
      961 tggcctcgat gcgcttgacg gcatcggcgg tcatgctctt gcggttcggc acgatcaggt
     1021 agcagtaggc gcggtggtgc gagcggccga cgcggccgcg caactggtgc agctgcgcca
     1081 gcccgagctt gtcggcgcgc tcgatgatga tggtgttggc gctcggcacg tcgatgccgg
     1141 attcgacgat ggtcgtgcac accagcaggt tgcagcgctg gtggtagaaa tcgagcatga
     1201 ccttttcgag ctcgccggcg cccatctggc cgtgcgcgac cgcgacgcgc gcgccgggca
     1261 cgagctgcgc gagttcctgc gcgcggcggt ggatgctgtc gacctcgttg tgcaggaagt
     1321 acacctgtcc gccgcgcttc agctcgcgca ggcaggcttc ctcgatcagg gctgcgttgt
     1381 actcgttgac gaaggtcttg accgcaagcc ggctcgccgg cggcgtggcg atgatcgaca
     1441 gctcgcgcag cccggcgagc gcgaagttca gcgtgcgcgg gatcggcgtg gcggtcagcg
     1501 tcaggatgtc gacctcggag cgcagggcct tgaaggcttc cttctggcgc acgccgaagc
     1561 ggtgttcctc gtcgatgatg acgagaccga ggtccttgaa ccgcacttcg ggctggatca
     1621 gcttgtgcgt gccgatgacg atgtcgatgc cgccgctggc gaggccggcg agcacggcgt
     1681 cggtgtcctt cttcgagcgg aagcgcgaca ggctctcgat ggtcaccggc cagccggcgt
     1741 agcggtcgag gaagttgcgg tagtgctggt tggcgagcag cgtcgtcggc accagcaccg
     1801 ccacctgccg gccatcctgc acggcgagga aggccgcgcg catcgcgacc tcggtcttgc
     1861 cgaagccgac atcgccgcag acgacgcggt ccatcggctt gccggtgcgc aggtcggcga
     1921 gcacggcgtc gatcgcgagc tgctggtccg gggtttcctc gtacggaaag gccgctgcga
     1981 aggcgagcat ctcgcgctcg tcgccgtgga atttgtgccc cgggcgggcg gcgcggcggg
     2041 cgtagacatc gagcagctcg gcggcggcat cgtgcgcctt ctcggcggcc ttgcgccgcg
     2101 ccttctccca ctgctcgccg ccgaggcggt gcagcggcgg gttctcgccg ccggtgtagc
     2161 ggctgacgcg atcgagcatc gccaccggca cgtagagttt ttcggcgttc gcgtattcga
     2221 gcacgaggta ctcggccggg atgccgccgg cctccatgtg cacgaggccc tgatagcggc
     2281 cgacgccgtg atcctcgtgc acgaccgccg cgccgatggc gaggtcggcg aggttgcgga
     2341 tgatggcctc ggcctcgcgc gcggcgcggc tgctgcgccg cgtgctgcgc acccattcgc
     2401 ggtgcagcag gctctcggtg atgacggcga gcggtgccgc ggtgccgtcc tcgagcacga
     2461 agccctgatc gagcggcgcg accgtcagcg cgagccgcgc ctcgctgccg acgaaacccg
     2521 gccagccgtc gaccgcgacc ggcagcagat tctgcgcacg cagcgtctcg gccagcacct
     2581 cgcgccggcc ggccgattcg gcggcgaaca gcacgcgccc cggaaaggca tcgagccagt
     2641 cgagcagtgc cgcggccggg cgctcggcgc gcggtgcgaa gtacagctcc ggcagcgccg
     2701 gggcgccggt gctgtcgaga tcgacgcgca gcagccggct gacgcggcgg ttgacctcgc
     2761 cggcgtcgag aaagagctgc gccggggcga gcagcgggcg cgtgcggtcg tggcggcggc
     2821 tctcgtagcg ctcctgcacc tgcgcgatga agccgtccat cgccgcttcc cagccgggca
     2881 ggcgcagcac cagcgcgttc gagggcagga agtcgaacag cgtcgaggtc tgctcgaaga
     2941 acagcggcag ccagtattcg atgccgggcg gcgcgaagcc ctggctgacc tcgcggtaga
     3001 tcgggctctg ctgcgggtcg ccctcgaagg tggcgcggaa gttcgtgcga aagcgcgtga
     3061 tcgattccct ggtcatcgcg aactcgcgcg ccggcagcag ctcgatcgca tcgagcttcg
     3121 cgatcgagcg ctgcgtctcg ggctcgaagc tcctcaggct ctcgatctcg tcatcgaaca
     3181 ggtcgatgcg cagcggcgcc tccgcgccca tcgggaacac gtcgatgatc gagccgcgca
     3241 ccgcgaactc gccgtgctcc atgacctgcg cgacgcagag ataaccggcc gcctccaggc
     3301 gcgtgcggaa ggcatcgaca tcgagccggt cgccggtggc gagcagcagc gcgtgcgcat
     3361 cgaggaagct cttcgggccg aggcgctgca tcagcgtcgg cgccggcacg atcagcacgc
     3421 cgcgcttctg catgggcagg cggtacagcg tcgccagccg ctgcgagatc agatcctgat
     3481 gcggcgagaa ctggtcgtac ggcagcgtct cccagtccgg gaacaccagc cgcggcacat
     3541 cgagcgtgcc ggcgaaccag ccgatcgccg catcgaggcg cagcgccgtc tgcgtgtccg
     3601 gcgccagcac cagcagcagc gcctgggtgc gcgccgccgt tgcggcgatc gccagcgcct
     3661 gcgcggcttc ggacaggccg ctccagcggc cgggcttcag cgcatcggcg ggcagcggca
     3721 gggcgggcag ggcggtggca gggctggcgg cggacttcgg cggcatcggc aagacacgtg
     3781 cggactgagg cggcaggcgc gggggcgcca tgatagccgc gtgccagagg ctggcggccg
     3841 atcgtgagga ctggttacga ccgatgtcgc gggcgcggcg gacagtgcag gctgaaaggc
     3901 gttgtcctgc ccgggcagcc tgagaatttt ctgataactt tttgcgaacc ggcgcagccg
     3961 gccggctgcg cagcctttcc agattcctcc ccccgagcag gttcccgccg cgatgatccg
     4021 cgtcatgctg gtcgacgatc accaactgat ccgcatcgcc ctcggccaga tcctcggcgg
     4081 ccacgccgac atcgaggtcg tcgccgaggc agccgacggc gaggaggcgc tggtgcgtgc
     4141 gcgcgccgcc aagcccgacg tgatcctgat cgatgtcgac atgcccggca tgggcggcgt
     4201 cgaggccacg cgccggctgt cggcgctcgc gcacaagccg cgcgtggtcg cgatctcggt
     4261 gcacgaccag atgccgtatc cgcagcggat gatggaggcc ggcgccgtcg gctacctgcc
     4321 caagggcggt tcggccgacg aggtgctggc cgcggtgcgc gccgtggccc gcggccagcc
     4381 gtacatcgcg ccggtgatcg ccggcaagat gttcctgacc tcgatgaacc gcagcggcag
     4441 cgccggcaac ggcgcgcagg tgctcgacac gctgtcggcg cgcgagatgc aggtgatgct
     4501 ggcgctggtc aacggccaga acccgcagca gatcggcgag gcgctgaaga tcagcgccaa
     4561 gaccgtctgc acgcaccgct atcacatcta cgagaagctc ggcgtcgaca gcgacgtggc
     4621 gctgacgcac ctcgcctacc gctacgggct gatcgaagtc tcgcagtcct gagccggacg
     4681 gcggcgggtc attcgtcgtc gtcgtcgtcg tcgtcgtgcg cgagcggcgc accctgcgcg
     4741 tcgaaatgca gcacgcggat ttcgccgtcg gcatcggtga tctcggcctg cgccagcgtg
     4801 ccgttcgcgt ggtaggcgta gcggtggcgc atctcgacct cgccgtagac gagcttctcg
     4861 atctcggtca cgcgctcgcc ggcatcgaac cagccgcgga agtaggtgtt gcggtgcgcg
     4921 gtgtcctcga tcgggccgct gagcttcagc ggcagccgga caccggaata ggtcacgaac
     4981 aggcggcagt ggccgcgtcc ggcagcggat tcagtttctg tggtcatcgg agccattccc
     5041 ggttgcaggg ggtcatgcac agggcgccgg cccggcggcg cgcagtctga cggccgccgc
     5101 cggcagcgtc cacactgagg gggccgggcc gggcgccgtg cgcagcgtcc gctcccgtcc
     5161 gcccgtcgcg tgcagccgag gtgccccgag atgcagaccg tcaccgccca ggaagacctg
     5221 cgcctgcgcg ccgcgctcga agtccagcgt taccggctcg ggcgctcgct gcgcggctgt
     5281 gccgccgccg cgctcaccgg cctgctgctc gcgctcggcg gcctgcacga ggaccggctg
     5341 ccgacgccgc tcgcgctcgg cttcctggtc acgctcggcg gcttcgcgag cctcggcgcg
     5401 ctgctgctgc agcggcgctt cgcgcggctc gcgcgcgtcg tgccgcggct gcgggccacg
     5461 ccctgtctgg cgctgctcgc ggtcggcggc tggttccagg agcccgagta ccaggccgat
     5521 gtgcgcatcc ccggcctctg gcactggcac gtcgatctcg aaaccccgct gcccgacccg
     5581 cacctcgaag ccggcggcga tcagccgatc gatgcgatcg cgtggctgga gccgcgccgg
     5641 caccggctgt gggcgctgga gctgcgcacc gtggtgttct gggtgcggcg ctgcgtgcgc
     5701 gtcgagccgg ccacgcgccg cgcctgaagc ccgcccgggc cgggccgggc ttcagcggcc
     5761 ccgcggtttc gcccgcgccg tcggcaccgc cgtccacgga tcgtccggcc acgggtgctt
     5821 cgggtagcgc cccttcagct ccttgcgcac ctcggcgtag gtgcgctccc agaagccgcg
     5881 caggtcctgc gtgacctgga tcgggcggcg ggccggcgac agcaggtgca gggtcacggc
     5941 gatgcggccg tcggcgatgc gcggcgtgtc ggcgaggccg aacatctcct gcagcttcac
     6001 ggccagcacc ggcgcggcac cgtcggcggc gtattcgagg cgcaggcgcg agccgctcgg
     6061 cacgtcgaga tgcgtcgggg ccagcgtggc gaggcgctgc gtctgcggcc agtcgaggcg
     6121 ggcgtgcagg atcgcggcga gatcgaggcg ggcgaggtgc tcgcggcgcg tgcagccgtc
     6181 gagatacggc tgcagccagt cgccgagcgt cgccgcgagc gcggcatcgg aaacctccgg
     6241 ccagccggcc tccggctgcc agtggcgcag cgactgcacg cgcttctgcc agtcgcgcag
     6301 cgcatcggtc cacggcagcg cgtcgaggcc gagcagctcg atcccggcgc acatggcccg
     6361 gcgcagcgcc gccggctcgg gacgcgcgag cgcgcgggcg tcgagcagga gcgcgccgag
     6421 ccggcactcg cgccgggcga cgacggcctg ctcgcgctcg tcccagcgca cctcggcgac
     6481 ctcggcgatg tggccggcga gggcggtctc cagcgtcggc aggtcgagcg ccacggcgag
     6541 gaagatgcgg ccttcgcgct cgccggcgtc gagcgcggcg accgcgagcc agtcgctgcc
     6601 ggtgagcgca tcgccctcgc gcagccgcgc accgcggccg ctggcgagca ggaaacgatc
     6661 cggactgccg tcgcggcgcc gggcgatgcg atccggatag gcggcggcga ccagcagcgc
     6721 caggtcctga ctgcggatcg acgcgcgcgg gtcggcggcc ggcacgtcga gccgctcgcg
     6781 cagcacgcgc gcggcttcga gcaggcgcgc gcaggcattg gcatcggcac cgaagccgcg
     6841 tgcggcctcg cgtgcaccgg cgcgcaccgc ggccagggct tcgaggcgaa gcgtcagatc
     6901 ggcgctgcgc gcggcctcgc cgcgcagcgg atcgcgctca tcgagcagcg cggcgagatc
     6961 acaggcgagc gcgccgagtc cgagcgcgcg cccgcgcagc agcaggtggc cgaggcgcgg
     7021 gtgcaggccg agcgcgagca gcgcgcggcc ctgcgcggtg at
//
LOCUS       DAOQIE010000208         7030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5645_length_7030_cov_7.529176, whole genome shotgun sequence.
ACCESSION   DAOQIE010000208 DAOQIE010000000
VERSION     DAOQIE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5645_length_7030_cov_7.529176"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK144_12730"
     CDS             <1..239
                     /locus_tag="PK144_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNF66635.1"
                     /translation="SWRCPAPITSTATTRRSRLLCVYGSDEKADSACPPLAGRNAQVE
                     ILALPGAHHFDGDYTALAKRLLDAAQAPAASNGK"
     gene            245..781
                     /locus_tag="PK144_12735"
     CDS             245..781
                     /locus_tag="PK144_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNF66636.1"
                     /translation="MTTTSTPTPAGPDSRLRSLHAAASTMLRLAALLAAGLAPVQADE
                     TIGREEVASLAYQGSRVRLYAIAAPRVDQICKEWRGGQPVDYACGQHAKAFLQSLVAG
                     RDPFCVREQAAGSASCYVDGRDIGLEMVAAGWAVARREESERYVYAQERAREQGRGLW
                     RGQFDDPTLLPQPDRRRR"
     gene            861..1088
                     /locus_tag="PK144_12740"
     CDS             861..1088
                     /locus_tag="PK144_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66637.1"
                     /translation="MQSLTLNLDLDDQILEDARARAQAEHTTLDEVMTRLLAEYARRE
                     QTLREYDAVVQRVCGTATVGRKLTRDEMNER"
     gene            1078..1503
                     /locus_tag="PK144_12745"
     CDS             1078..1503
                     /locus_tag="PK144_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNF66638.1"
                     /translation="MSVESFIDTNVFIYHLEKADARRFMIADTIIREAIATGSACISF
                     QVVQECLNTMLRKASIPLDTAGMRDYLDIVLLPLVRVPASADLYRRSLEVRERYGFSF
                     YDALIVAAALEAGCTRLWSEDLQHGQRIGVLQVENPFRA"
     gene            1561..4368
                     /locus_tag="PK144_12750"
     CDS             1561..4368
                     /locus_tag="PK144_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF66639.1"
                     /translation="MLSDPLILGSALFYIGLLFAVAYWGDRAAEAGRSVIANPWTYTL
                     SLGVYCTAWTFYGSAGRAAHGGIAFLPVYLGPTLVLVLGWQLVRRIVRISQRNRITSI
                     ADLIGSRYGKSHLLAGLVTVIAVIGIMPYISIQLKAIATSYTVLRGYPEVSPLVRVAE
                     QPVWADTTLYAALALIAFAVLFGTRHIDNTERHEGLVAAIAFESVVKLGAFLLVGAYV
                     VWSLNDGFGDIFTRAAAHPELAKLMRFDAIGGGYAGWVTLTLLSACAFLFLPRQFQVL
                     VVENTDEHHLRTASWAFPLYLWLINLFVLPVALAGLMTFESRGLDADLFVLDLPIHGR
                     EPFLAVLVFVGGLSAATSMVIVETVALSTMVCNDLVMPLLLRRLRPGGRRDLSGLLLG
                     IRRIAIAGIVLLGYLYYRVIGESYTLVSIGLVSFAACAQFAPVILAGIYWKGATRRGA
                     YWALSAGFAVWAYTLLLPSFARSGWLPEGFVTDGPFGIELLKPYALFGLAGLDQYSHA
                     LVWSWLANVGGLLLGSLWDRPGALERVQASQFVDAWDSSSTARLLWRGTADIHELQTL
                     VARFLGPERAASAFAGYAQSRGLEPGRHGRAEAELVHFAEHLLAGAIGAASARVTVSS
                     VVKGEALSIEEVMTILDETSQAIQYSRRLEEQSRELEAAGRELRAANARLQELDRLKD
                     EFVSTVTHELRTPLTSIRAFAEILLGAPDIPDTQRAEFLGIIVKESERLSRLINQVLD
                     MAKLDSGRVDWRSEPVELRALLRDAANATSQLFRDKGAELTLDLDEAPAAGADTADGS
                     LAGAHALHLAQQPAVITGDSDRLQQVLINLLSNAVKFCPEVHGRVAVRLRVQENGVRI
                     EVADNGPGIPLAEQARIFEKFHQVSSQQAGKPKGTGLGLTISQRIVERHGGTIRVESI
                     EGEGACFIVELPAQVLANAAA"
     gene            4444..5283
                     /gene="dapF"
                     /locus_tag="PK144_12755"
     CDS             4444..5283
                     /gene="dapF"
                     /locus_tag="PK144_12755"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNF66640.1"
                     /translation="MNTRSLRFSKMHGLGNDFVVIDGVRQPVTLDADLVRRLGDRHFG
                     VGFDQLLLVEPPTRPGMDFRYRIWNADGGEVEQCGNGARCFARFVRDHGLSDKDAIAV
                     ETAGGDLVLHVEADGRVTVNMGAPRFEPADIPFEAPARALHYAVEQDEIPYFLSVLSM
                     GNPHAVLTVDSVAHAPVTTLGPKLEHHPRFPKRVNVGFIEGIDRSHVRVRVWERGAGE
                     TLACGTGACAAVVAGRLQGWLDERVEVELRGGRLTICWAGEGEPVYMTGPAVRVFDGT
                     LNF"
     gene            5373..6113
                     /locus_tag="PK144_12760"
     CDS             5373..6113
                     /locus_tag="PK144_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNF66641.1"
                     /translation="MSTPTPLPPDPATEAGVAEWLAGHPDFFERHPELLEALSVPHLL
                     PPGVASLVERQVLLLRARETATRRKLAQLIEIARANDRLAARMHAFALGIVGLTTLED
                     LLAAITGLMRDEFGADAVALRLCAQPRDGTGGDERFVPPALWSEYAQALDGPRPACGP
                     LPPALAGRLFDAASGIASCALLPLRGCGWDGLLAIGARDAERYGAGLGTLFLQRIAEL
                     VAHTLDVFLDRCPDTGADADPDARTGDA"
     gene            6091..>7030
                     /gene="xerC"
                     /locus_tag="PK144_12765"
     CDS             6091..>7030
                     /gene="xerC"
                     /locus_tag="PK144_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNF66642.1"
                     /translation="MRGPAMREAVEAWLATLTHERKLSPHTLAAYRHDLDEFLGWCAT
                     RALSGWPMIGHAEIRAWVATQHRQGLSGRSLQRRLAALRGLYEHLRRAGLVADNPAKL
                     VQAPKSPKKLPRALDVDQTARLLDAAPDSTLETRDRAMLELLYSSGLRLAELIALDLA
                     DLPTGADEVRVLGKGSKERIVPVGAQARAALGDWRQVRGQLAAPGEPALFVGRDGQRL
                     GRTGVARALSRWARKRGDGTHVHPHLLRHSFASHLLESSGDLRAVQELLGHADLATTQ
                     VYTHLDYQHLARVYDSAHPRARRGTGVKDGDGNGNGE"
BASE COUNT          982 a         2591 c         2417 g         1040 t
ORIGIN      
        1 aatcctggcg ctgcccggcg cccatcactt cgacggcgac tacacggcgc tcgcggctgc
       61 tgtgcgtcta cggcagcgac gagaaggccg actcggcctg cccgccgctc gccgggcgca
      121 acgcgcaggt ggaaatcctg gcgctgcccg gcgcccatca cttcgacggc gactacacgg
      181 cgctcgcgaa acgcctgctc gatgccgcgc aggcaccggc tgcaagcaac gggaaatgac
      241 gatgatgaca accacttcca cccccacgcc ggccggaccc gacagccgcc tgcgctcgct
      301 gcacgctgca gcatcgacga tgctgcggct tgccgcgctg cttgcggccg ggctcgcgcc
      361 ggtacaggcc gacgagacga tcggacgcga ggaggtcgcg agcctcgcgt atcagggttc
      421 gcgcgtgcgc ctgtacgcga tcgccgcacc gcgcgtcgac cagatctgca aggagtggcg
      481 cggcggccag ccggtcgact acgcctgcgg ccagcacgcc aaggccttcc tgcagagcct
      541 ggtcgccggg cgcgatccgt tctgcgtgcg cgagcaggcc gccggcagcg cgagctgcta
      601 cgtcgatggc cgcgacatcg gcctggagat ggtcgccgcc ggctgggccg tggcgcggcg
      661 cgaggaatcc gagcgctacg tctatgcgca ggagcgcgcc cgcgagcagg gccgcggcct
      721 ctggcgcggc cagttcgacg atccgacgct gctgccgcag cccgaccgcc gccggcgctg
      781 acgcgtgcag gcaggtgcgg acggcggcat tacactgccg gcaggtcccg ccgcaccctg
      841 cccgaccgga gtccgccgcc atgcagagcc tgacgctgaa cctcgacctc gacgatcaga
      901 tcctcgaaga cgcccgtgcc cgcgcgcagg ccgagcacac gacgctcgat gaagtgatga
      961 cgcgtctgct cgccgaatac gcccggcgcg agcagaccct gcgtgaatac gatgccgtcg
     1021 tgcagcgggt ctgcggcacg gcgacggtcg gccgcaaact gacgcgggac gagatgaatg
     1081 agcgttgaga gcttcatcga caccaacgtc ttcatctatc acctcgaaaa ggccgatgcg
     1141 cgccggttca tgatcgccga cacgatcatt cgcgaggcga tcgccaccgg cagtgcctgc
     1201 atcagcttcc aggtcgtgca ggaatgcctc aacacgatgc tgcgcaaggc ctcgatcccg
     1261 ctcgacacgg cgggcatgcg tgactatctc gacatcgtgc tgctgccgct ggtgcgggtg
     1321 ccggcgagcg ccgatctgta ccggcgcagc ctcgaggtcc gcgagcgcta cggcttcagc
     1381 ttctacgacg cgctgatcgt cgccgcggcg ctcgaagccg gctgcacccg gctctggagc
     1441 gaggacctgc agcacggcca gcgcatcggt gtgctgcagg tcgaaaaccc gttccgcgcc
     1501 tgagcgtctg ctccggcact cccgatcccg atcccgttcc gcccggccga aggtgccgcg
     1561 atgctctccg atccgctgat cctcggcagt gcgctgttct acatcggctt gctgttcgcg
     1621 gtcgcgtact ggggcgatcg tgccgccgag gccgggcgct cggtgatcgc caatccgtgg
     1681 acgtacacgc tgtcgctcgg cgtctactgc acggcctgga ccttctacgg cagcgccggg
     1741 cgcgccgcgc acggcgggat tgccttcctg ccggtgtacc tggggccgac gctggtgctc
     1801 gtgctcggct ggcagctggt gcggcgcatc gtgcgcatca gccagcgcaa ccgcatcacg
     1861 tcgatcgccg acctgatcgg ctcgcgctac ggcaagagcc atctgctcgc cgggctggtg
     1921 acggtgatcg cggtgatcgg gatcatgccg tacatctcca tccagctgaa ggcgatcgcc
     1981 accagctaca cggtgctgcg cggctatccg gaagtctcgc cgctggtgcg cgtcgccgag
     2041 cagccggtgt gggccgacac cacgctgtac gcggcgctgg cgctgatcgc gttcgcggtg
     2101 ctgttcggca cccgccacat cgacaacacc gaacgccacg agggcctggt cgcggcgatc
     2161 gcgttcgagt cggtggtcaa gctcggcgcc ttcctgctgg tcggggccta cgtggtctgg
     2221 agcctcaacg acggcttcgg cgacatcttc acgcgcgccg ccgcgcaccc ggaactcgcg
     2281 aagctgatgc gcttcgatgc gatcggcggc ggctacgccg gctgggtgac gctgacgctg
     2341 ctgtcggcct gcgccttttt attcctgccg cgccagttcc aggtgctggt ggtcgagaac
     2401 accgacgagc accacctgcg caccgcgagc tgggcctttc cgctgtatct gtggctgatc
     2461 aacctgttcg tgctgccggt ggcgctcgcc gggctgatga ccttcgagag ccgcggcctc
     2521 gatgccgacc tgttcgtgct cgacctgccg atccacggcc gcgagccctt cctcgccgtg
     2581 ctggtgttcg tcggcgggct gtcggcggcg accagcatgg tcatcgtcga gaccgtggcg
     2641 ctgtcgacga tggtctgcaa cgacctggtg atgccgctgc tgctgcgccg gctgcgcccg
     2701 ggcgggcggc gcgacctctc cgggctgctg ctcggcatcc gccgcatcgc catcgccggc
     2761 atcgtgctgc tcggctacct gtactaccgg gtgatcggcg agtcctacac gctggtgtcg
     2821 atcggcctcg tgtcgttcgc ggcctgcgcg cagttcgcac cggtgatcct cgccggcatc
     2881 tactggaagg gcgcgacgcg ccgcggtgcc tactgggcgc tgtcggccgg cttcgcggtc
     2941 tgggcgtaca cgctgctgct gccgtcgttc gcgcgctcgg gctggctgcc cgagggcttc
     3001 gtcaccgacg ggccgttcgg catcgagctg ctgaaaccct acgcgctgtt cggcctcgcc
     3061 gggctcgacc agtactcgca cgcgctggtg tggagctggc tcgccaacgt cggcggcctg
     3121 ctgctcggct cgctgtggga ccgccccggt gcgctggagc gcgtgcaggc cagccagttc
     3181 gtcgatgcct gggacagcag cagcactgcc cggctgctgt ggcgcggcac cgccgacatc
     3241 cacgagctgc agacgctggt cgcgcgcttc ctcggtccgg agcgcgccgc cagcgccttc
     3301 gccggctatg cgcagagccg cggcctcgaa ccgggccgcc acggccgcgc cgaggccgag
     3361 ctcgtgcact tcgccgagca cctgctcgcc ggcgcgatcg gcgcggcctc ggcgcgggtg
     3421 acggtgtcct cggtggtcaa gggcgaggcg ctgtcgatcg aggaggtcat gaccatcctc
     3481 gacgagacct cgcaggccat ccagtacagc cgccggctcg aagagcagtc gcgcgaactc
     3541 gaagccgccg gccgggagct gcgcgccgcc aacgcccggc tgcaggagct cgaccggctc
     3601 aaggacgaat tcgtgtcgac ggtcacgcac gagctgcgca cgccgctgac ctcgatccgt
     3661 gccttcgccg agatcctgct cggcgccccc gacatccccg atacgcagcg cgccgagttc
     3721 ctcggcatca tcgtcaagga gagcgagcgc ctgagccggc tgatcaacca ggtgctcgac
     3781 atggccaagc tcgattccgg gcgcgtcgac tggcgcagcg agccggtcga gctgcgcgcg
     3841 ctgctgcgcg atgccgccaa cgccaccagc cagctgttcc gcgacaaggg cgcggagctg
     3901 acgctcgatc tcgacgaggc cccggccgcc ggtgcggata cggccgacgg cagcctcgcc
     3961 ggtgcgcacg cgctgcacct cgcgcagcag ccggccgtga tcaccggcga cagcgaccgg
     4021 ctgcagcagg tgctgatcaa cctgctgtcg aacgccgtga agttctgccc cgaagtccac
     4081 ggccgggtcg cggtgcgcct gcgcgtgcag gagaacggcg tgcgcatcga ggtcgccgac
     4141 aacggtcccg gcatcccgct cgccgagcag gcgcgcatct tcgagaagtt ccaccaggtc
     4201 agcagccagc aggccggcaa gcccaagggc accggcctcg ggctgaccat cagccagcgc
     4261 atcgtcgagc gccacggcgg cacgatccgc gtcgagagca tcgagggcga aggcgcctgc
     4321 ttcatcgtcg agctgccggc gcaggtgctg gcgaacgcgg cggcctgagc accgcctgaa
     4381 cgccgcgcag atcgtgttgg cgcaagcctc ggcacccgcg accgccttgg ttacactcgg
     4441 ttcatgaaca cgcgatccct gcgcttttcc aagatgcacg gcctcggcaa cgacttcgtc
     4501 gtcatcgacg gcgtgcgcca gccggtcacg ctcgacgccg atctcgtgcg ccgcctcggc
     4561 gaccggcact tcggcgtcgg tttcgaccag ctgctgctgg tcgagccgcc gacccggccg
     4621 ggcatggatt tccgctaccg catctggaac gccgatggcg gcgaggtcga gcagtgcggc
     4681 aacggcgcac gctgcttcgc gcgcttcgtg cgcgatcatg gcctgagcga caaggacgcc
     4741 atcgccgtcg agaccgccgg cggcgatctg gtgctgcacg tcgaggccga cgggcgcgtc
     4801 accgtcaaca tgggcgcgcc gcgcttcgag ccggccgaca tcccgttcga ggcaccggcg
     4861 cgcgcgctgc actacgccgt cgagcaggac gagattccgt acttcctgag cgtgctgtcg
     4921 atgggcaacc cgcacgcggt gctgaccgtc gattcggtcg cgcacgcacc ggtgacgacg
     4981 ctcggcccga agctcgaaca tcacccgcgc ttcccgaagc gcgtcaatgt cggcttcatc
     5041 gaaggcatcg accgcagcca cgtgcgcgtg cgcgtctggg agcgcggtgc cggcgagacg
     5101 ctcgcctgcg gcaccggcgc ctgcgcggcc gtggtcgccg gccggctgca gggctggctc
     5161 gacgagcgcg tcgaggtcga gttgcgcggc ggccggctga cgatctgctg ggccggcgaa
     5221 ggcgagccgg tatacatgac cggtccggcc gtgcgcgtgt tcgacggcac gctgaacttc
     5281 tgatccgcac ggacctgccc gcatccgtgc agcgccgcgc gatccatgcg gatctgcccg
     5341 atcctcccgc ccccgtctcc cggagcgccg cgatgagtac accgacaccg ctgccgcccg
     5401 accccgccac cgaagccggc gtcgccgaat ggctggccgg gcatccggac ttcttcgagc
     5461 gccatcccga actgctcgaa gccctgagcg tgccgcacct gctgccgccc ggggtcgcgt
     5521 cgctggtcga acgtcaggtg ctgctgctgc gcgcgcgcga aaccgcgacg cgccgcaagc
     5581 tcgcgcagct gatcgagatc gcccgcgcca acgatcgcct ggcggcgcgc atgcatgcgt
     5641 tcgcgctcgg catcgtcggc ctgacgacgc tcgaagacct gctcgccgcc atcaccggcc
     5701 tgatgcgcga cgagttcggc gccgacgcgg tggcgctgcg gctgtgtgcg cagccgcgcg
     5761 acggcacggg cggcgacgag cgcttcgtgc cgccggcgct gtggagcgag tacgcgcagg
     5821 ccctcgacgg cccgcgcccg gcctgcggcc cgctgccgcc ggcgctggcc gggcggctgt
     5881 tcgacgccgc gagcggcatc gcctcctgcg cgctgctgcc gctgcgcggc tgcggctggg
     5941 acggcctgct cgcgatcggc gcccgcgacg ccgagcgcta cggcgccggc ctcggcacgc
     6001 tgttcctgca gcgcatcgcc gaactggtcg cgcacacgct cgatgtcttt ctcgaccgct
     6061 gccccgacac cggcgccgac gccgatcccg atgcgcggac cggcgatgcg tgaggccgtc
     6121 gaagcctggc tcgcgacgct gacgcacgaa cgcaagctgt cgccgcacac gctcgccgcc
     6181 taccgccacg acctcgacga attcctcggc tggtgcgcga cgcgcgcgct gtccggctgg
     6241 ccgatgatcg gccacgccga gatccgcgcc tgggtggcca cgcagcaccg gcagggattg
     6301 tccgggcgca gcctgcagcg gcggctggcg gcgctgcgcg ggctctacga acacctgcgc
     6361 cgcgccggcc tcgtcgccga caacccggcg aaactcgtgc aggcgccgaa gtcgccgaag
     6421 aagctgccgc gcgcgctcga tgtcgaccag accgcccgcc tgctcgacgc cgcccccgac
     6481 agcacgctcg aaacgcgcga ccgcgccatg ctcgaactgc tgtattcctc cggcctgcgg
     6541 ctggccgaac tgatcgcgct cgatcttgcc gacctgccga ccggtgccga cgaggtgcgc
     6601 gtgctcggca agggcagcaa ggaacgcatc gtcccggtcg gtgcccaggc ccgcgccgcg
     6661 ctcggcgact ggcgtcaggt gcgcggccag ctcgccgccc ccggcgagcc ggcgctgttc
     6721 gtcggccgcg acggccagcg gctcggccgc accggcgttg cgcgcgcact gtcgcgctgg
     6781 gcgcgcaagc gcggcgacgg cacgcacgtg cacccgcacc tgctgcgcca cagcttcgcg
     6841 agccacctgc tcgaatcgag cggcgacctg cgcgccgtgc aggaactgct cggccacgcc
     6901 gacctcgcga ccacgcaggt ctacacccac ctcgactacc agcacctcgc cagggtctat
     6961 gactcggcgc atccgcgcgc ccggcgcgga acgggggtga aggacgggga cggcaatgga
     7021 aacggggaag
//
LOCUS       DAOQIE010000209         7021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5664_length_7021_cov_6.694660, whole genome shotgun sequence.
ACCESSION   DAOQIE010000209 DAOQIE010000000
VERSION     DAOQIE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_7021_cov_6.694660"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK144_12770"
     CDS             complement(<1..496)
                     /locus_tag="PK144_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66643.1"
                     /translation="MTDGSGEACCSDNPVQSLDKLAPDEVSAPKSCGLHAFSADIAPK
                     SCRHLVPNHFVEARNLLVEAFCSGVEAFCCDDLVQNLDEEARNDLFGLRKAGCRVKNL
                     DIASMWCKNHPMNVDSEGREMRPAEAGTDCRPALPGMPVTTAPTTPGLVPAAAVPTLA
                     DFCDC"
     gene            1098..1343
                     /locus_tag="PK144_12775"
     CDS             1098..1343
                     /locus_tag="PK144_12775"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNF66644.1"
                     /translation="MSTLDELTRIGRELPESALREVLDFARFLQNRNAGSASTERAQR
                     LLEALEVAVRLDPFAGLDGVQWQQAQRAERPLPGRDA"
     gene            1340..1708
                     /locus_tag="PK144_12780"
     CDS             1340..1708
                     /locus_tag="PK144_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF66645.1"
                     /translation="MILADSNLLIYAIQPEYGALRVWLAASLPKVSAVSVVEVLGYHR
                     LNAAERAALETVFAALDVLYPGPETVRLAVGLRQQRRMSLGDALIAATALEYGLRLAT
                     HNLRDFDGIAGLDAYSPLAG"
     gene            complement(1723..4827)
                     /locus_tag="PK144_12785"
     CDS             complement(1723..4827)
                     /locus_tag="PK144_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF66646.1"
                     /translation="MVLPDLSIRRPVFATVMSLMIVLIGFIAYTRLAVREYPNIDAPV
                     VSVRTVYKGASAEIIESRVTQVLEDSLAGIEGVKQMKSVSREEVSQITVEFKLERNID
                     AAANDVRDKVSRVRAQLPDDIDEPVIAKIEADAQAILWLAFSSDTQTALEITDYADRN
                     VKDRLQTLPGVASVIIGGERRYAMRLWIDRDRLAAYALTPADVEDALRRQNVEIPAGR
                     IESSQREFSVLAAADLTTAAQFEKLIIKEANGYPIRLSDVGRAELGAADERNIVRYNG
                     RNAVGLGIVKQSSANTLAVAQAVKALLPVIRDGLPAGMRVDIGFDSTVFIEKAIESVY
                     HTMVEAMVLVILVIFFFLRSVRATLIPFVTIPVSLVGACIFLYALGFSINVLTLLGMV
                     LAIGLVVDDAIVVLENIHRHIEEGMTPFRAALKGSAEIAFAVVAMTLTLAAVFTPLAF
                     ATGNTGRLFTEFALAVAAAVLVSGFVALTLTPMMCSRLLKGGHAQHGRVFLAIEHGIE
                     GMTRGYVRVLSASLRARWLVLILFAGTTLVCAWLFTQMKSELSPVEDRGFIIGVVVAP
                     EGSTLAYTDGYARQIETFLNDIPELNNYFAVVAPGLERPNPVNSALSFIRFKPWEERA
                     RSQQDIAKSLAPKMFGLPGVLAFPINPPSLGQSFRNPPVQFVIQANSYPELSRNVDAL
                     LAKLRQYPGLANVDSDLRLNKPQLTVQLKRDKIADVGVRVDDIGRALETLLGGRQVTR
                     FKREGRQYDVIVQLGAGDRVTPADLEAIFVRGSNDRLVQLSNLVEVKETVAAKELNHF
                     NRLRASVISANLAPGTTLGEALDHLDATAAEVLGPTRATALDGQSREFRESGGTLYIT
                     FVLALAVIYLVLAAQFESFVSPFVIMLTVPLAMTGALAALLMTGGTLNVYSQIGMVML
                     VGLITKNGILIVEFANQLRAQGSDRFAAVTEAARLRLRPILMTTAAMVLGAVPLALAT
                     GAGAESRQQIGWVIVGGLSLGTLLTLFVVPAFYTLLVKSVHVPGREDPDGEESEVGGQ
                     VAVR"
     gene            complement(4834..5919)
                     /locus_tag="PK144_12790"
     CDS             complement(4834..5919)
                     /locus_tag="PK144_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF66647.1"
                     /translation="MFRFELSALRRLLRALPSVTVLAVAPPLHAQQAGGPPATPVKVV
                     PVARETVIETLTAVGTLRANESVLIRPEIAGRIQTIHFREGEPVAAGAPLVTLDPAEY
                     KAQVAGSQADVTLSELNFERQRNLDRSRLASQQDLDNARAKLDQARAQLDLDRVRLDK
                     TVIHAPFAGTVGLRAVSPGAYVSPGQDIARLDSLNPIKLDFRVPEVQLARLKVGQPVA
                     VRIDAYAERDFRGEILAIDPTLDEITRTVLVRARLANAEGLLRPGVFARVQLEIARRE
                     QALVIPEEAIWPVGRQTLVYVVKDGKAESVPVELGLRQAGKVEVKNGLAADAQVVTDG
                     QMKLRPGAAVKIAEPAAPPAAGARQGG"
     gene            complement(6110..6550)
                     /locus_tag="PK144_12795"
     CDS             complement(6110..6550)
                     /locus_tag="PK144_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66648.1"
                     /translation="MSTLPRPAAAALLAAIALATGCAPPAGGYPYGNAVPAWKVVGLG
                     TSTCAGFSATARGTAEREAYRQWLLGYLSAINVGSPTTADVIDSRHLNWTDIVKPQNW
                     EEWLEGWCRTQPGAPFQTAAQMVVMREYNEMLKQDARENSRDRR"
     gene            complement(6590..>7021)
                     /locus_tag="PK144_12800"
     CDS             complement(6590..>7021)
                     /locus_tag="PK144_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF66649.1"
                     /translation="AQIALGAQPAGQHRLPGRVGMALTGGLIGAVSGVVGIGGGSLTV
                     PFLTWCQVPIRNAVASSAAVGLPIAVSGALGYVAAGWNEPLLPSGALGYVSLTAGAGI
                     AAASMLTAPLGARLAHTLPTTLLRRIFAGFLALVGLKMLFG"
BASE COUNT         1091 a         2301 c         2547 g         1082 t
ORIGIN      
        1 gacagtcgca gaagtccgcg agtgtaggga cggcggcggc ggggaccaag ccgggagtgg
       61 tcggggcggt ggtgacgggc attcccggga gcgcaggccg gcagtcggtg ccggcttcgg
      121 ccgggcgcat ctcgcgacct tcgctgtcga cgttcatggg gtggtttttg caccacattg
      181 atgcaatgtc caggtttttc accctgcagc cggcctttcg aagcccgaaa aggtcgttcc
      241 gagcctcctc atcgaggttc tgaacgaggt cgtcgcagca aaaagcctcg acgccggagc
      301 aaaaagcctc gacgaggagg ttccgagctt cgacgaagtg gttcggaacg aggtgtctgc
      361 agctttttgg tgcaatatcg gcgctaaatg cgtgcagtcc gcagcttttt ggtgcggaaa
      421 cctcgtccgg agctagcttg tcgaggctct gaacagggtt gtcgctgcag caagcctcgc
      481 cggacccatc cgtcatcccg aggccggagt tcgcagcctc gacggcgaag cccggagctg
      541 ccgcgaggca cctcgcagcg aagccgtgca ggcccgcgca ctcggacggg aggctcggcg
      601 ggacaaggac agggttcggg gaacgcttcg actcgcaggc tgggttgacg aaggaaaccc
      661 agcactcgat gcacgcgagg acaacgaatt ggttgcgctg gtgatcaagc ttttgattgc
      721 tgctttttac tttgccaaaa gccgggtttc gttcctcaag ccagcctgcg cgtgccggtg
      781 agtctgccga aaagaacccg tgaccttccg tgggcgaccg tgtgatgtct tgtccgggag
      841 ccggatgcgg gagacccgca cgtccggttc gatgagcggg gtgtggaaac ggagctacgg
      901 caagactgcc tgggcaccgc cagacgaaag gggcggaaac aggcaagtcg aacctgtcgc
      961 caccgcgcct catcccgact ccacttgctc gccccgcgtt cgtgcccatc atggcaggcg
     1021 gagcgacgca gcagcgtgca cactggcggc acgcgcatcg gctgccggtg gcagcggcat
     1081 ctgacggagg cactcgcatg agcacgctgg acgaactgac ccgcatcggg cgcgaactgc
     1141 cggaatccgc gctgcgcgag gtgctggact ttgcccggtt cctgcaaaac cggaatgccg
     1201 ggtcggcttc gacggaacgc gctcagcgac tgctcgaggc gctggaggtg gccgtgcgcc
     1261 tcgatccgtt cgccggcctc gacggtgtgc aatggcagca ggcacagcgt gccgagcggc
     1321 cgctgccggg gcgggatgcg tgatcctcgc cgacagcaat ctcctgatct acgcgatcca
     1381 gcccgagtac ggtgcgctgc gcgtctggct ggcggccagc ttgccgaagg tctcggcggt
     1441 cagcgtcgtg gaggtactgg gctaccaccg gctgaacgca gcggaacggg cagccctgga
     1501 aacggtattc gcggccctcg acgtgctgta tccgggaccg gagacggtgc ggcttgctgt
     1561 cggtttgcgt cagcagcgac gcatgagcct cggcgatgcg ctgatcgcgg cgaccgcgct
     1621 ggaatacgga ttgcggttgg cgacgcacaa tctccgcgat ttcgacggca tcgccggact
     1681 tgatgcctac tcgccgctgg ccggttgatc cacggcgttc cttcaacgca ccgccacctg
     1741 cccccccacc tcgctttcct ccccatccgg gtcttcccgt cccggcacat gcacgctctt
     1801 gacgagcaat gtatagaacg ccggcaccac gaacagcgtc agcagcgtgc cgagtgacag
     1861 gccgccgacg atgacccagc cgatctgctg gcgggattcg gcgccggcgc cggtggcgag
     1921 cgcgagcggg acggcgccaa ggaccatggc ggcggtggtc atcaggatcg ggcgcaggcg
     1981 caggcgggcg gcttcggtga cggcagcgaa gcggtcgctg ccctgggcgc gtaactggtt
     2041 ggcgaattcg acgatcagga tgccgttctt cgtgatcagg ccgaccagca tgaccatgcc
     2101 gatctgcgaa tagacgttca gcgtgccgcc ggtcatgagc agcgccgcga gcgcgccggt
     2161 catcgccagc ggcacggtca gcatgatcac gaacggcgac acgaaactct cgaactgcgc
     2221 ggcgagcacg agatagatca cggcgagcgc cagcacgaag gtgatgtaca gcgtgccgcc
     2281 ggattcgcgg aattcgcgcg actggccatc gagcgcggtc gcgcgcgtcg ggccgagcac
     2341 ctcggcggcg gtggcgtcga ggtgatcgag cgcctcgccg agcgtcgtgc cgggtgcgag
     2401 gttcgccgag atcacgctcg cgcgcaggcg gttgaagtgg ttgagttcct tggcggcgac
     2461 ggtttccttc acctcgacga ggttggaaag ctgcaccagc cggtcattgg agccgcgcac
     2521 gaagatcgct tcgaggtcgg ccggcgtcac gcggtcgccg gcaccgagct gcacgatcac
     2581 gtcgtactgg cggccctcgc gcttgaagcg cgtgacctgc cggccgccga gcagggtttc
     2641 gagcgcgcgg ccgatgtcat cgacgcgcac gccgacgtcg gcgatcttgt cgcgcttcag
     2701 ctgcacggtg agctgcggct tgttcaggcg caggtccgaa tcgacgttgg ccaggcccgg
     2761 gtactggcgc agcttcgcga gcagcgcatc gacgttgcgc gacagctccg gatagctgtt
     2821 ggcctggatc acgaactgca ccggcgggtt gcggaagctc tgcccgagcg agggcggatt
     2881 gatcgggaag gcgagcacgc cgggcaggcc gaacatcttc ggcgccagcg acttcgcgat
     2941 gtcctgctgg ctgcgcgcgc gttcttccca gggcttgaag cggatgaacg acagcgcgct
     3001 gttgaccgga ttcggccgct ccagccccgg cgcgaccacg gcgaagtagt tgttcagctc
     3061 cgggatgtcg ttcaggaagg tctcgatctg gcgcgcgtag ccgtcggtgt aggcgagcgt
     3121 cgagccttcc ggcgcgacga cgacgccgat gatgaaaccg cggtcctcga ccggcgacag
     3181 ctccgatttc atctgcgtga acagccaggc gcagaccagc gtggtgccgg cgaacaggat
     3241 cagcaccagc cagcgcgcgc gcagcgaggc cgaaagcacg cgcacgtagc cgcgcgtcat
     3301 gccctcgatg ccgtgctcga tggcgaggaa cacgcggccg tgctgggcgt ggccgccctt
     3361 cagcagccgc gagcacatca tcggcgtcag cgtcagcgcc acgaagcccg acaccagcac
     3421 cgccgccgcg accgcgagcg cgaactcggt gaacagccgc ccggtgttgc cggtcgcgaa
     3481 ggcgagcggc gtgaacaccg cggcgagcgt cagcgtcatc gccacgaccg cgaaggcaat
     3541 ctcggccgag cccttgagcg ccgcgcgaaa cggcgtcatg ccttcctcga tgtggcggtg
     3601 gatgttctcc agcacgacga tggcgtcatc gaccaccagc ccgatcgcca gcaccatgcc
     3661 gagcagcgtc agcacgttga tcgagaagcc gagcgcgtac aggaagatgc aggccccgac
     3721 cagcgacacc gggatggtca cgaacgggat cagcgttgcg cgcacgctgc gcaggaagaa
     3781 gaagatcacg aggatcacga gcaccatcgc ctcgaccatc gtgtggtaga cggactcgat
     3841 ggccttctcg atgaacaccg tgctgtcgaa gccgatgtcg acgcgcatgc cggccggcag
     3901 gccgtcgcgg atgaccggca gcagcgcctt gaccgcctgc gcgaccgcga gcgtgttggc
     3961 gctcgactgc ttgacgatgc cgaggccgac ggcgttgcgg ccgttgtagc gcacgatgtt
     4021 gcgctcgtcg gcggcgccga gttcggcgcg cccgacatcg gagagccgga tcggatagcc
     4081 gttggcttcc ttgatgatga gcttctcgaa ctgcgcagcc gtggtcaggt cggcggcggc
     4141 gagcaccgag aactcgcgct ggctggattc gatgcgcccg gccggaatct cgacgttctg
     4201 acggcgcagg gcgtcctcga catcggccgg ggtcagcgcg taggcggcga ggcggtcgcg
     4261 gtcgatccag aggcgcatcg cgtagcggcg ctcgccgccg atgatcaccg aggccacgcc
     4321 gggcagcgtc tgcaggcggt ccttgacgtt gcggtcggcg tagtcggtga tctccagcgc
     4381 cgtctgcgtg tcgctcgaga acgccagcca caggatcgcc tgcgcgtcgg cctcgatctt
     4441 ggcgatcacc ggctcgtcga tgtcgtccgg cagttgcgcg cgcacgcgcg agaccttgtc
     4501 gcgcacgtcg ttggcggcgg cgtcgatgtt gcgttcgagc ttgaactcga cggtgatctg
     4561 gctgacttcc tcgcgcgaca ccgacttcat ctgcttgacg ccttcgatgc cggccagcga
     4621 atcctccagc acctgcgtca cgcgcgattc gatgatctcg gcgctcgcgc ccttgtagac
     4681 cgtgcgcacc gacacgaccg gcgcgtcgat gttcgggtac tcgcgcacgg cgaggcgcgt
     4741 gtacgcgatg aagccgatca gcacgatcat caggctcatg accgtcgcga acaccggccg
     4801 gcggatcgaa agatcaggca gcaccatggc ggctcagccc ccctgtctgg cgccggcggc
     4861 cggcggcgcg gccggttcgg cgatcttcac ggcggcgccg gggcgcagct tcatctggcc
     4921 gtcggtgacg acctgcgcat cggcggcaag cccgttcttc acctcgacct tgccggcctg
     4981 acgcaggccg agttcgaccg gcacgctctc ggccttgccg tcctttacga cgtagaccag
     5041 cgtctggcgg ccgaccggcc agatggcctc ctcggggatc accagcgcct gctcgcggcg
     5101 cgcgatctcc agctgcacgc gcgcgaacac gccggggcgc agcaggccct cggcgttggc
     5161 gagccgggcg cgcaccagca ccgtgcgcgt gatctcgtcg agcgtcgggt cgatggcgag
     5221 gatctcgccg cggaagtcgc gctcggcgta ggcgtcgatg cgcaccgcga ccggctggcc
     5281 gaccttcagg cgcgcgagct gcacctcggg cacgcggaaa tcgagcttga tcggattcag
     5341 gctgtcgagc cgggcgatgt cctggcccgg cgacacatag gcgccggggc tgaccgcgcg
     5401 caggccgacc gtgccggcga agggcgcgtg gatcacggtc ttgtcgaggc gcacgcggtc
     5461 gaggtcgagc tgcgcgcgcg cctgatcgag cttggcgcgg gcgttgtcga ggtcctgctg
     5521 gctggcgagg cggctgcggt cgaggttgcg ctggcgctcg aagttcagct cgctcagcgt
     5581 cacgtcggcc tggctgccgg cgacctgcgc cttgtattcg gccgggtcga gcgtcaccag
     5641 cggcgcgccg gcggcgaccg gctcgccctc gcggaagtgg atggtctgga tgcggccggc
     5701 gatctcgggg cggatcagca ccgactcgtt ggcgcgcagc gtgccgaccg cggtcagcgt
     5761 ctcgatgacg gtctcgcgcg cgaccggcac gaccttgacc ggcgtcgccg gcggcccgcc
     5821 ggcctgctgg gcgtgcagcg gcggcgcgac ggcgagcacg gtgacggagg gcagcgcgcg
     5881 cagcaggcgg cgcagggcgg agagctcaaa gcggaacatc ggcggcggtt cctcgcttgc
     5941 gggcgacagg cgtccggcca cgacgggcca cggatgcgcg agatgcggcg agcggccgga
     6001 gccggccgtg cgccgttacg ggcggggatt gtgcgcgcgt cgggcgaggg ctccggctct
     6061 gcgcgccggt atggctacca tgcaggagcg gccgaagcgt gcggcgtggt cagcgccggt
     6121 cgcggctgtt ctcgcgggcg tcctgcttca gcatctcgtt gtactcgcgc atgacgacca
     6181 tctgggccgc ggtctggaac ggcgcgccgg gctgcgtgcg gcaccagcct tccagccatt
     6241 cctcccagtt ctgcggcttg acgatgtcgg tccagttcag gtggcgagag tcgatcacgt
     6301 cggccgtggt cggcgagccg acgttgatcg cgctgagata cccgagcagc cactgccggt
     6361 aggcctcgcg ttcggccgtg ccgcgcgccg tggcgctgaa gccggcgcag gtcgaggtgc
     6421 cgaggccgac gaccttccag gccggcacgg cgttgccgta cggatagccg ccggcgggag
     6481 gcgcgcagcc ggtggcgagg gcgatggcgg cgagcagtgc ggcggcggcg gggcggggca
     6541 gggtggacac gagggcagtc ctctggccgg tgacggagac ggtcgcgctt cagccgaaca
     6601 gcatcttcag tccgaccagc gcgaggaagc ccgcgaagat gcgtctgagc agcgtcgtcg
     6661 gcagcgtatg cgcgagtctg gcaccgagcg gcgccgtcag catgctcgcc gcggcgatgc
     6721 cggcgccggc cgtgaggctg acgtagccga gcgcgcccga gggcagcagc ggctcgttcc
     6781 agccggcggc gacgtagccg agcgcgccgg acaccgcgat cggcaggccg acggcggccg
     6841 agctggcgac ggcgttgcgg atcggcacct ggcaccaggt caggaagggc acggtcagcg
     6901 agccgccgcc gatgccgacg acgccggaca ccgcgccgat gaggccgccg gtgagcgcca
     6961 tgccgacgcg ccccggcagc cggtgctggc cggccggctg cgcgccgagc gcgatctgcg
     7021 c
//
LOCUS       DAOQIE010000210         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5668_length_7018_cov_6.911963, whole genome shotgun sequence.
ACCESSION   DAOQIE010000210 DAOQIE010000000
VERSION     DAOQIE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_7018_cov_6.911963"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PK144_12805"
     CDS             <1..493
                     /locus_tag="PK144_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNF66650.1"
                     /translation="FGPVWATSHLGDDSISLIGTDPTRHKAQAWKVVRTLKGQGGGSL
                     FIKTHPQSSHLYVDTALNPDPKISQSTLVYDLKDLARAPVVLPIADWAELGEGPKRVV
                     QPEYNKDGNEVWFSVWNGKDQKSALVVVDDRTLKLKTVIKDAKLITPTGKFNVYNTQH
                     DVY"
     gene            794..1564
                     /locus_tag="PK144_12810"
     CDS             794..1564
                     /locus_tag="PK144_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNF66651.1"
                     /translation="MSTPPSPRSPRPRKVAMYEDVAERVRQKIYDHELAPGDWIDEPA
                     LAAEFGISRTPLRECLKLLAAEGLVQIDPGRGASVTKLTLEDLNELFPVMAMLEGRCA
                     FECARKLDAERLRTLETLHEALETAAAAGDVAAYYASNYVFHRTVQDFAGNPWLTRVT
                     NDLRRILKMHRGRQLLLPGRLQESLSEHRALIECFRSHDAGRAERVMNQHLLHQRVAL
                     VRYIESGGSLNGPARLPDTPGAASAPNPPYPNPQDAIA"
     gene            1937..2356
                     /gene="madL"
                     /locus_tag="PK144_12815"
     CDS             1937..2356
                     /gene="madL"
                     /locus_tag="PK144_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HNF66652.1"
                     /translation="MVIYGVALLSICTLIGMFAGDLLGQLIGVKANVGGVGFAMLLLI
                     LISDRLRRDLKLVPMSQRGIEFWSAMYIPIVIAMASTQNVAAAIKGGPAALLAGVIAT
                     IACAALIPVLDRWGRPKPAVSGNSASLSTTVIASGER"
     gene            2362..3126
                     /gene="madM"
                     /locus_tag="PK144_12820"
     CDS             2362..3126
                     /gene="madM"
                     /locus_tag="PK144_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HNF66653.1"
                     /translation="MYETLETLLGKNGLLTAFAVVGATMWLSYWLSDRLTRGRIHGSA
                     IAILAGLVLAYVGGVAAGGKNGLADLKLFSGLGLMGGAMLRDFAIVATAFGVSFDELK
                     KCGLSGVLSLLVGVVVSFVLGAAVAYGFGYTDAVSMTTIGAGAVTYIVGPVTGAAIGA
                     SSEVIALSIAAGLVKSVLVMIGTPFVAKWIGLDNPRTAMIFGGMMGTTSGVCGGLAAT
                     DPKLVPYGAMTSTFYTGLGCLMGPSVLFLATRAMFG"
     gene            3278..4942
                     /gene="mdcA"
                     /locus_tag="PK144_12825"
     CDS             3278..4942
                     /gene="mdcA"
                     /locus_tag="PK144_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HNF66654.1"
                     /translation="MNAPLTPPRRWDTQSQNRAARLGRAAAFARGRHVAAADARALLE
                     AVLEPGDRVCLEGNNQKQADFLAKTLVTVDPARVHDLHMVQSVLALPEHIDVFERGIA
                     TKLDFSFSGPQGARLAKLVSAGRIDIGAIHTYLELFGRYFTDLTPRVSLIAAQAADAA
                     GNLYTGPNTEDTPVIVEATAFKRGIVIAQVNEVVDTLPRIDIPADWVDFVIPAPTPAY
                     VEPLFTRDPAQISEVQVLMAMMAIQGIYAEYGVQRLNHGIGFDTAAIELLLPTYGESL
                     GLKGKICRHWALNPHPALIPAIEAGWVESVHSFGGELGMEAYTAARPDVFFTGPDGSM
                     RSNRALCQAAGHYACDLFIGSTLQIDLAGNSSTATLGRIAGFGGAPNMGADARGRRHA
                     SPAWLKAGEQARAGSSRMPRGQKLVVQMVETFREHMAPAFVEQLDAWELAEQAGMPLP
                     PVMIYGEDVTHILTEEGIANLLLCRTAEEREQAIRGVAGYTAVGLGRDRRAVENLRDR
                     GVIRRPEDLGIDKRLASRDLLAAKSMKDLVRASGGLYDPPKRFRNW"
     gene            5111..5425
                     /gene="mdcC"
                     /locus_tag="PK144_12830"
     CDS             5111..5425
                     /gene="mdcC"
                     /locus_tag="PK144_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase acyl carrier protein"
                     /protein_id="HNF66655.1"
                     /translation="METLRFEYLGGTPPAVQAPTLTGVVGSGNLEVLVEAAPGSGSCV
                     IEITTAAVGFGHIWQAVIDDFHARHGLCDLRISVNDVGATPAVVSLRLDQAIETFLGD
                     AA"
     gene            5422..6309
                     /locus_tag="PK144_12835"
     CDS             5422..6309
                     /locus_tag="PK144_12835"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0044262 - cellular carbohydrate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HNF66656.1"
                     /translation="MNAHRHSYYEAGARERLHGLLDAGSFRELLGPAERMTSPHLPPL
                     DAPVAFDDGVVVGRGRLAGRSVLAGAQEGGFNGGAVGEVHGAKLTGLLERALDERPAA
                     VLLLVESGGVRLHEANAGLIAVSEVMRAVLAVRSAGIPVLVLDGGQFGCFGGMGIVAR
                     CCDAIVISEEGRLGLSGPEVIETTCGVEEFDSRDRALVWRTVGGKHRYLLGEADVLVA
                     DELAAFREAAIRLLDTVPDLSLDALDAEHDRLAARLERFGTCRDALDIWSALGIGQPA
                     RLPLLDTDEFLTATADRRA"
     gene            6313..>7018
                     /gene="mdcE"
                     /locus_tag="PK144_12840"
     CDS             6313..>7018
                     /gene="mdcE"
                     /locus_tag="PK144_12840"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044262 - cellular carbohydrate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HNF66657.1"
                     /translation="MDARTLLDQLFPAGHDVRFDGAFFHGSGRSGTTDIAVIGTVDAA
                     PIGVELALALAGAVLDTVRRHPGRPILLAVDTAGQRLSHRDELLGINGYMAHLAKCLT
                     LARNRGHKVLGLVYSKAVSGGFLASSLLADACHALPGAEVMVMNLPAMARVTKIPLEK
                     LQDLSRSSPVFAPGVANYVAMGAVESVWQGNLAEALAAALAGMPQHDRRRELGEARGG
                     RRLARPVAARVRSDALA"
BASE COUNT         1051 a         2627 c         2318 g         1022 t
ORIGIN      
        1 gttcggtccg gtctgggcca ccagccacct cggcgatgac agcatctcgc tgatcggcac
       61 cgacccaacc agacacaagg cccaggcctg gaaggtcgtg cgcacgctga agggtcaggg
      121 gggcggttcg ctgttcatca agacccaccc gcagtcgagc cacctgtacg tcgacaccgc
      181 gctgaacccg gatccgaaga tcagccagtc gacgctggtc tacgacctga aggatctcgc
      241 cagagcgccg gtggtgctgc cgatcgccga ctgggccgag ctcggcgagg gtccgaagcg
      301 cgtcgtgcag cccgagtaca acaaggacgg caacgaggtc tggttctcgg tctggaacgg
      361 caaggaccag aagtcggcgc tcgtcgtcgt cgacgaccgg acgctgaagc tcaagaccgt
      421 catcaaggac gcgaagctga tcacgccgac cggcaagttc aacgtctaca acacgcagca
      481 cgacgtgtac tgagccagca gcgagccgct ccccggcgga tcgggggggg aagcgtcgcg
      541 ccagtctcac ggcgggggcg gaccggtcct gcggtccgcc cccgctttat tttcgcggcg
      601 accgtcccgg ccggctgcgg ttcttccggc tccgcagccg ccccggcgcg ccgatcgttc
      661 ccggctccgg ctgccgatcc tccccggcgc gcctgcgcgc gcatgccctg aaaaaatcta
      721 agccgcggct tgtatttttt cataattatg atgtaatcac accatcatca ccgaaccgcg
      781 gagtccccgg atcatgagca ccccgccttc cccccgcagc ccgcgcccgc gcaaggtcgc
      841 gatgtacgag gatgtcgccg agcgcgtgcg ccagaagatc tacgaccacg aactcgcccc
      901 cggcgactgg atcgacgaac cggcgctcgc cgccgagttc ggcatcagcc gcacgccgct
      961 gcgcgagtgc ctgaagctgc tcgccgccga ggggctggtg cagatcgacc cggggcgcgg
     1021 tgccagcgtc accaagctca cgctcgaaga cctcaacgag ctgttcccgg tcatggcgat
     1081 gctcgaaggc cgctgcgcct ttgaatgcgc gcgcaagctc gatgccgagc gcctccggac
     1141 gctcgaaacc ctgcacgagg cgctcgagac cgccgccgcc gccggcgacg tggccgcgta
     1201 ctacgcgagc aactacgtct ttcaccgcac ggtgcaggac ttcgccggca acccgtggct
     1261 gacgcgcgtg accaacgacc tgcgccgcat cctgaagatg caccgcggcc gccagctgct
     1321 gctgccgggg cggctgcagg aatcgctgtc cgaacaccgc gcgctgatcg agtgcttccg
     1381 gagccacgac gccgggcgcg ccgagcgcgt gatgaaccag cacctgctgc accagcgcgt
     1441 cgcgctggtc cgctacatcg aatccggcgg cagcctcaac ggcccggcgc ggctgccgga
     1501 cacgcccggc gccgccagtg caccgaatcc gccgtatccg aacccgcagg acgccatcgc
     1561 ctgatgcgct gcccgccgcc cgcgccgaca gagcgcccgc cggagtctcc ggcaggaccg
     1621 ggcggcagac caccgaggag ccccgtcatg tcgcgacctc ccgccacacc gccgccccgc
     1681 ggctgattcc cccgcttcac cgatcccgga acgccgtccg accggcgcgc gcaggctgcg
     1741 tgcgcccggt ccctgccggc cgcgctccgg ccccgaaccg cccgaccgag gaggtgcccc
     1801 gcacccgccc gccgatccgt cgtgcccgtc ttcgcccgtc gtgcccggag acatccctga
     1861 gaacaggcgg ccggagagcc gtcgctccaa acctgcacaa cctgccccac ccgcccacca
     1921 agcgaggaga tttgccatgg tcatctacgg agtagccctg ctgtcgatct gcacgctgat
     1981 cggcatgttc gccggcgacc tgctcggcca gctcatcggc gtcaaggcca acgtcggcgg
     2041 cgtcggattc gcgatgctgc tgctgatcct gatctcggac cggctgcgcc gggacctgaa
     2101 gctcgtaccg atgtcgcagc gcggcatcga gttctggagc gcgatgtaca tcccgatcgt
     2161 catcgcgatg gcctcgacgc agaacgtcgc cgccgcgatc aagggcggtc cggccgcgct
     2221 gctggccggc gtcatcgcga cgatcgcctg cgcggcgctg attccggtgc tggatcgctg
     2281 gggccggccg aagccggcgg tgagcggcaa cagcgcctcc ctgtccacga cggtcatcgc
     2341 ctccggcgaa cggtgagcat catgtacgaa accctggaaa ccctgctcgg caagaacggc
     2401 ctgctgaccg cattcgcggt ggtcggcgcc accatgtggc tgtcgtactg gctgtccgac
     2461 cgcctgaccc gcggccgcat ccacggctcg gcgatcgcga tcctcgccgg tctcgtgctc
     2521 gcctacgtcg gcggcgtcgc cgccggcggc aagaacggcc tggccgacct caagctgttc
     2581 tccggcctcg gcctgatggg cggcgcgatg ctgcgcgatt tcgccatcgt cgcgaccgcg
     2641 ttcggcgtca gcttcgacga actgaagaag tgcgggctga gcggcgtgct gtcgctgctg
     2701 gtcggggtgg tcgtgtcctt cgtgctcggg gccgcggtcg cgtatggctt cggctacacc
     2761 gacgccgtca gcatgaccac gatcggcgcc ggtgccgtga cctacatcgt cggcccggtc
     2821 accggcgcgg cgatcggcgc gagttccgag gtcatcgcac tcagcatcgc tgccggtctg
     2881 gtcaagtcgg tgctggtcat gatcggcacg cccttcgtcg cgaagtggat cggcctcgac
     2941 aacccgcgga ccgcgatgat cttcggcggc atgatgggca cgaccagcgg cgtctgcggc
     3001 ggtctggccg cgaccgatcc gaagctcgtg ccctacggcg cgatgacctc gaccttctac
     3061 accggcctcg gctgcctgat gggcccgtcg gtgctgttcc tcgccacgcg ggcgatgttc
     3121 ggctgaaagc tgccggcacg gtgccggtcc gtcccccttc gtgtccgtaa tttcaagata
     3181 attttattaa gatcatcttg tcatcacgga aaccggacgg accgcaccgg caggcaccac
     3241 cccgatcccc tgccctgctc caaggagtgc gatgaccatg aatgcccccc tcaccccccc
     3301 gcgccgctgg gacacgcaat cgcagaaccg cgccgcccgc ctcggacgcg ccgccgcttt
     3361 cgcccgcggc cggcacgtcg ccgccgccga tgcccgcgcg ctgctcgaag ccgtgctgga
     3421 gcccggcgac cgcgtctgcc tcgaaggcaa caaccagaag caggccgact ttctcgcaaa
     3481 gacgctggtc acggtcgacc cggcgcgcgt gcacgacctg cacatggtgc agtcggtgct
     3541 ggcgctgccc gagcacatcg acgtgttcga gcgcggcatc gcgacgaagc tcgacttctc
     3601 gttctccggc ccgcagggcg cgcggctcgc gaagctcgtc agcgccgggc gcatcgacat
     3661 cggcgcgatc cacacctacc tcgaactgtt cggccgctac ttcacggacc tcaccccgcg
     3721 cgtcagcctg atcgccgcgc aggccgccga tgccgccggc aacctctaca ccggcccgaa
     3781 caccgaggac acgccggtga tcgtcgaggc gacggccttc aagcgcggca tcgtcatcgc
     3841 gcaggtcaac gaggtcgtcg acacgctgcc gcgcatcgac atcccggccg actgggtgga
     3901 cttcgtgatc ccggcgccga ccccggccta cgtcgagccg ctgttcacgc gcgatccggc
     3961 gcagatcagc gaggtgcagg tgctgatggc gatgatggcc atccagggca tctacgccga
     4021 atacggcgtg cagcgcctga accacggcat cggcttcgat acggcggcga tcgagctgct
     4081 gctgccgacc tacggcgagt cgctgggcct gaagggcaaa atctgccggc actgggcgct
     4141 gaacccgcat ccggcgctga tcccggcgat cgaggccggc tgggtggagt ccgtgcattc
     4201 cttcggcggc gaactcggca tggaggccta cacggcggcg cgccccgacg tgttcttcac
     4261 cggcccggac ggcagcatgc gttcgaaccg cgcgctgtgt caggccgccg gccactacgc
     4321 ctgtgatctg ttcatcggct cgacgctgca gatcgatctc gccggcaaca gctcgaccgc
     4381 gacgctcggg cgcatcgccg gcttcggcgg cgcgccgaac atgggcgccg atgcacgcgg
     4441 ccgccggcac gcgagtccgg cctggctcaa ggccggcgag caggcgcgcg ccggcagcag
     4501 ccggatgccg cgcggccaga agctcgtcgt gcagatggtc gagaccttcc gcgagcacat
     4561 ggcaccggcc ttcgtcgaac agctcgatgc ctgggaactg gccgagcagg ccggcatgcc
     4621 gctgccgccg gtgatgatct acggcgagga cgtcacccac atcctgaccg aggaaggcat
     4681 cgccaacctg ctgctctgcc gcacggccga ggagcgcgaa caggcgatcc gcggtgtggc
     4741 cggctacacg gcggtcggac tcggccgcga ccggcgtgcc gtcgagaacc tgcgcgaccg
     4801 cggcgtgatc cgccggcccg aggacctcgg catcgacaag cgcctcgcga gccgcgacct
     4861 gctggccgcg aagtcgatga aggacctcgt gcgcgcctcc ggcggcctgt acgacccgcc
     4921 gaagcgcttc cgcaactggt gatgccgatg cgcggtgcct gcggcaggac gcatcgaagc
     4981 gcggtttcgc ccccgctcgc caacccctct cccgcgaggg ccgagggact gaaagacgcg
     5041 ctcccgccgt gcgcccgcct cacccccggc cgtccgacgg ccaccggcct gaacctcccg
     5101 gagacccgtg atggaaacct tgcgttttga atacctcggc ggcacgccgc ccgccgtcca
     5161 ggccccgacg ctgaccggcg tggtcggctc cggcaacctc gaagtgctgg tcgaggccgc
     5221 gcccggctcc ggcagctgcg tgatcgagat cacgaccgcc gcggtcggct tcggccacat
     5281 ctggcaggcc gtcatcgacg acttccacgc ccgccacggc ctctgcgacc tgcgcatctc
     5341 ggtcaacgat gtcggcgcga cgccggccgt cgtcagcctg cgcctcgacc aggccatcga
     5401 aaccttcctc ggagacgccg catgaacgcg caccgccaca gctactacga agccggcgcc
     5461 cgcgagcgcc tgcacggcct gctcgatgcc ggcagcttcc gcgaactgct cggtccggcc
     5521 gagcgcatga ccagcccgca cctgccgccg ctcgatgcac cggtggcctt cgatgacggc
     5581 gtcgtcgtcg gccgcgggcg gctcgccggg cgcagcgtgc tggccggcgc gcaggaaggc
     5641 ggcttcaacg gcggcgcggt cggcgaggtg cacggcgcca agctcaccgg cctgctcgaa
     5701 cgcgcgctcg acgagcgccc ggccgcggtg ctgctgctgg tcgagtccgg cggcgtgcgc
     5761 ctgcacgagg ccaacgccgg gctgatcgcg gtgtccgagg tcatgcgcgc cgtgctcgcg
     5821 gtgcgcagcg ccggcatccc ggtgctggtg ctcgacggcg gccagttcgg ctgcttcggc
     5881 ggcatgggca tcgtcgcgcg ctgctgcgat gcgatcgtca tcagcgagga aggccggctg
     5941 ggcctgtccg gccccgaggt catcgagacc acctgcggcg tcgaggaatt cgactcgcgc
     6001 gaccgggcgc tggtctggcg caccgtcggc ggcaagcacc gctatctgct cggcgaggcc
     6061 gacgtgctgg tcgccgatga gcttgctgcg ttccgcgagg ccgcgatccg cctgctcgac
     6121 accgtgccgg acctgtcgct cgacgcgctc gacgccgaac acgaccgcct cgctgcacgc
     6181 ctcgaacgct tcggcacctg ccgcgatgcg ctcgacatct ggagcgcgct cggcatcgga
     6241 cagccggcgc gcctgccgct gctcgacacc gatgaattcc tcaccgccac cgccgaccgg
     6301 agggcctgaa ccatggacgc ccgcaccctg ctcgaccagt tgttccccgc cggccacgac
     6361 gtgcgcttcg acggcgcctt cttccacggc agcggcagaa gcggcacgac cgacatcgcc
     6421 gtgatcggca ccgtcgatgc cgcgccgatc ggcgtcgaac tggcgctcgc gctcgccggc
     6481 gccgtgctcg acaccgtgcg ccgccacccg ggccggccga tcctgctcgc agtcgatacc
     6541 gccggccagc gcctgtcgca ccgcgacgag ctgctcggca tcaacggcta catggcgcat
     6601 ctggccaagt gcctgacgct cgcgcgcaac cgcggccaca aggtgctcgg cctcgtgtac
     6661 tcgaaggccg tcagcggcgg cttcctcgcc agcagcctgc tcgccgatgc ctgccacgcg
     6721 ctgcccggcg ccgaggtcat ggtcatgaac ctgccggcaa tggcgcgcgt gaccaagatc
     6781 ccgctcgaaa agctgcagga cctgagccgg agctcgccgg tgttcgcacc gggcgtcgcc
     6841 aactacgtgg cgatgggcgc cgtcgagtcg gtctggcagg gcaatctcgc cgaggcgctg
     6901 gccgctgcgc ttgccggcat gccgcagcac gaccgccgcc gcgaactcgg cgaggcgcgc
     6961 ggcggccggc ggctggcgcg gccggtcgcg gcccgggtgc gcagcgatgc gctggcct
//
LOCUS       DAOQIE010000211         7014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5673_length_7014_cov_7.177755, whole genome shotgun sequence.
ACCESSION   DAOQIE010000211 DAOQIE010000000
VERSION     DAOQIE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_7014_cov_7.177755"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1169)
                     /gene="flhB"
                     /locus_tag="PK144_12845"
     CDS             complement(42..1169)
                     /gene="flhB"
                     /locus_tag="PK144_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNF66658.1"
                     /translation="MAEEDGQERTEEPTPRRLEQAREKGQIPRSRELSTALLTLGGSV
                     ALVSMGAGYVDTLLELMRAGFALEWRDIFEPTRPAQALGHALLTMLGLLAPFLLLMWV
                     LALLAPIAIGGWNWSAESLAPKWERLDPLTGIGRLFSLHALAELGKALAKFLLVGSVA
                     IWVLHDAEPELLGLAAEPLPLALAHAGTRIGELALWLAAPLLLIAGFDVPFTLWQHAK
                     ELRMTLQEVKDEMKDIEGRPEIKGKLRRMQMEFAQRRMMDQVPGADAVIVNPEHYAVA
                     LKYEAETMKAPKVLALGIDEIALRIRALAGASGVPVVESPLLARALYYNAKIDRDIPV
                     ALYLAVAQVLAYVFQLRADHELPGAPIRMPEVEVPAGVRTE"
     gene            complement(1171..1953)
                     /gene="fliR"
                     /locus_tag="PK144_12850"
     CDS             complement(1171..1953)
                     /gene="fliR"
                     /locus_tag="PK144_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNF66659.1"
                     /translation="MPPLTSSEIAGWVGGFVWPLFRIAAMLSVAPVIGARSLPRRVRA
                     ALALVLTAVTVPLLPPGPQVEPMSPAGILIGVQQVLIGLYIGFALRLAFAALETGGQM
                     ISTQMGLGYASLIDPQNGASTPLLAQFYGLLGMLIFLGLDGHLLLIETVVESFHLLPV
                     STDGLALQRWRELADAGAMIFAGAVKFALPAMASLLVVNLAFGVMTRAAPQLNIFAVG
                     LPVTLLLGLAILLYSLPALAPEFRRLIDAAFLLVHRALAAGG"
     gene            complement(1976..2245)
                     /gene="fliQ"
                     /locus_tag="PK144_12855"
     CDS             complement(1976..2245)
                     /gene="fliQ"
                     /locus_tag="PK144_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNF66660.1"
                     /translation="MTPETVITLGQRTLEVSALLSAPLLGAMLIVGLVVGLFQAATQI
                     NEQTLSFVPKLIVAVLVIVAGGGWMIEILTDFTERLYRSIPTLIG"
     gene            complement(2268..2990)
                     /gene="fliP"
                     /locus_tag="PK144_12860"
     CDS             complement(2268..2990)
                     /gene="fliP"
                     /locus_tag="PK144_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296595.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNF66661.1"
                     /translation="MCAATALALAEPRVPAVTMTPGTGGVQEWSVSLQILAIMTALTL
                     LPSLVLMTTAFTRIVIVLALLRQALGTMQTPSNQVLIGLALFLTVFIMGPVLQRMHDE
                     ALTPYLEERIGFQEAVEKARVPLSQFMLAQTRESDLALFAKLSGRESFESPESVPFTL
                     LVPAYVTSELKTAFQIGFMLFIPFLIIDFVVSSVLMAMGMMMLSPLVISFPFKLMLFV
                     LVDGWPLVMGTLAASFHGPGSG"
     gene            complement(3022..3516)
                     /gene="fliO"
                     /locus_tag="PK144_12865"
     CDS             complement(3022..3516)
                     /gene="fliO"
                     /locus_tag="PK144_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNF66662.1"
                     /translation="MTETRHRTPRAAWRLPALASLFAAGRAAAAPETQPPAAPAFDVS
                     SLVNLGIKLLVVIAVILALAWLARQVPTLRRSSGPITILGGLNVGNRERIVLVEVHGE
                     RVLLGVTPGSVTTLHVLDRLAVHGAGAGETDGEDSPRRRFDFRSALADYHRAREDGGG
                     PPHP"
     gene            complement(3516..3992)
                     /gene="fliN"
                     /locus_tag="PK144_12870"
     CDS             complement(3516..3992)
                     /gene="fliN"
                     /locus_tag="PK144_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNF66663.1"
                     /translation="MSEPEDRPEGAEDSASPAAATEPAAASSAPPPKPAARPAAPPPK
                     PAAATRNSGGFGTNFDTAGDPVPSANGRAANLDVVLDIPITLSMELGRTRLSIRELLA
                     LSQGNVVRLDRPAGEPLDILVNGCLVARGEVVVVNDRFGVRIVEIVSPEERIKRLR"
     gene            complement(3989..5047)
                     /locus_tag="PK144_12875"
     CDS             complement(3989..5047)
                     /locus_tag="PK144_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNF66664.1"
                     /translation="MPNRDLLSQDELDALMEGVGNGRVVVGRGQSAGEVLAYDLASPE
                     NVIACIQPALNTVQMRFVHQLGLGFYELLRRDLPLTPVEVHAEPYQDFVARLHPPCSV
                     NVIGLSPMAGPGLILFDHPLVFSLVGAWYGGQGTQYSETDRLEFTAAELRMVQRLNAL
                     CAKALVSAWQPYLPVRWTQHASEISPHLVTAIHPGELLIVFGIDLMLDGTLGTLRVAL
                     PSSMFEPVRGTLLAALAREHPLRDERLQRLLEEGVRDAQVQVSCVLAELEVSVNDLLN
                     LQVGDTIPVDLPDQARLEVDGVRLLAGHFGDTQGWRALQIDRWLVSLPETFELPSAER
                     ALKPVGRDKAPAKAERKP"
     gene            complement(5090..5659)
                     /locus_tag="PK144_12880"
     CDS             complement(5090..5659)
                     /locus_tag="PK144_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNF66665.1"
                     /translation="MAKKEEAKPAEAAAAGGGGGGKGKLIIIILLVLILLILAVGATL
                     FFTGALNKFLGHGGDDGSAVAAGGHGAPMAVPVAVPKSAGPAKYQPLDPPFVVNFDDQ
                     GVLRYVQISVSIMARDQKALDKVKENDPQIRYRLILLFSGQTFAELGTPAGKEKLQQK
                     TLEEIQKILIAETGTPGIEAVYFTNFVMQ"
     gene            6004..>7014
                     /locus_tag="PK144_12885"
     CDS             6004..>7014
                     /locus_tag="PK144_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66666.1"
                     /translation="MSSTEIAAAAPGRGWGRARRVAAAFVARNIEFVRDRTALGWNLV
                     MPVLIVLGFAFGYSGEPAPQYKIGLLNDGAAHPAFLDTRHVQFVPYAEADTEAAITRV
                     ERHQLDLLIDLRTQHYWVNEESAKGYLAERLLAGSDGGSGFRREAVRGRVIRYVDWLI
                     PGVLSMNMMWSALYGVGYVIVRYRKNGVLKRLKATPLSAVEFLAAQIGSRLTLVLLVS
                     TLVFTGTHLLIGYPMYGSYLHLLLVFALGASSLIGIGLVIAARLRTEEMADGLLNLIS
                     WPMMFLSGIWFSLDGMHPWVQKLALIFPLTHVTTAARAVMIDGAGLATIAPQLGVLAG
                     FTL"
BASE COUNT         1099 a         2484 c         2412 g         1019 t
ORIGIN      
        1 gggcggcggc ccccgccagc ccgccggcgc cggcgggagc tctactcggt gcgcacgccc
       61 gccggcacct cgacctccgg catccggatc ggcgcgcccg gcagttcgtg gtcggcgcgc
      121 agctggaaca cgtaggccag cacctgcgcg accgccaggt acagcgccac cggaatgtcg
      181 cgatcgatct tggcgttgta atacagcgcg cgcgcgagca gcggcgattc gaccaccggc
      241 acgccgctgg ccccggcgag cgcgcggatg cgcagcgcga tctcgtcgat gccgagcgcc
      301 agcaccttcg gcgccttcat ggtctcggcc tcgtacttca gcgccaccgc gtagtgttcg
      361 gggttgacga tcacggcatc ggcgcccggc acctgatcca tcatgcgccg ctgcgcgaac
      421 tccatctgca tgcgccgcag cttgcccttg atctcgggcc ggccctcgat gtccttcatc
      481 tcgtccttga cctcctgcag cgtcatgcgc agctccttgg cgtgctgcca cagcgtgaac
      541 ggcacgtcga agccggcgat cagcagcagc ggcgcggcca gccacagcgc aagctcgccg
      601 atgcgcgtgc cggcgtgcgc gagcgcgagc ggcagcggtt cggcggcgag cccgagcagt
      661 tcgggctcgg catcgtgcag cacccagatc gcgacgctgc cgaccagcag gaacttcgcg
      721 agcgccttgc cgagttcggc gagcgcgtgc agcgagaaca gccggccgat gccggtcagc
      781 ggatccagcc gctcccactt cggcgcgagc gattcggccg accagttcca gccgccgatc
      841 gcgatcggcg cgagcagcgc cagcacccac atcagcagca ggaagggcgc gagcagcccg
      901 agcatcgtca gcagcgcgtg cccgagcgcc tgcgccggcc gggtcggctc gaagatgtcg
      961 cgccactcca gcgcgaagcc ggcgcgcatc agttcaagca gcgtatcgac gtagcccgcg
     1021 cccatcgaca ccagcgccac gctgccgccg agcgtcagca gcgccgtgct cagctcgcgc
     1081 gagcgcggga tctggccctt ctcgcgtgcc tgttcgagcc gtcggggggt gggttcctcg
     1141 gtgcgttcct gtccgtcttc ctcggccatc tcagcctccg gccgccagcg cccggtgcac
     1201 gagcaggaag gcggcgtcga tcaggcgacg gaattccggc gcgagcgccg gcagcgaata
     1261 cagcaggatc gccagcccga gcagcagcgt caccggcagc ccgaccgcga agatgttcag
     1321 ctgcggcgcg gcgcgggtca tgacgccgaa ggcgaggttg acgaccagca gcgaggccat
     1381 cgccggcagc gcgaacttca cggccccggc gaagatcatc gcgccggcat cggcaagctc
     1441 gcgccagcgc tgcagggcca ggccgtcggt cgacaccggc agcaggtgga agctctcgac
     1501 cacggtctcg atcagcagca ggtgaccgtc gaggccgagg aagatcagca tgccgagcag
     1561 gccgtagaac tgcgcgagca gcggcgtcga ggcaccgttc tgcggatcga tcagcgaggc
     1621 gtagccaagc cccatctgcg tcgagatcat ctgcccgccg gtttcgagcg ccgcgaacgc
     1681 caggcgcagt gcaaagccga tgtacaggcc gatcagcacc tgctgcacgc cgatcaggat
     1741 gccggccggc gacatcggct cgacctgcgg ccccggcggc agcagcggca cggtcaccgc
     1801 cgtcagcacc agcgccagtg ccgcgcgcac gcgccgcggc agcgagcgcg caccgatcac
     1861 cggcgcgacc gacagcatcg cggcgatccg gaacagcggc cacacgaaac cgccgaccca
     1921 gccggcgatc tcgctgctcg tcagcggcgg catcggggcg aatccggcac gcgcatcagc
     1981 cgatcagcgt cgggatgctg cggtacaggc gctcggtgaa gtcggtcagg atctcgatca
     2041 tccagccgcc gccggcgacg atgaccagca ccgcaacgat cagcttcggc acgaaggaca
     2101 gcgtctgctc gttgatctgc gtcgccgcct ggaacaggcc gacgaccagg ccgacgatca
     2161 gcatcgcccc gagcagcggc gccgacagca gcgccgagac ctccagcgtg cgctggccga
     2221 gcgtgatcac ggtttccgga gtcatggcag caaggcctcg cgtgcgttca gccgctgccc
     2281 ggtccgtgga agctcgcggc cagcgtgccc atgaccagcg gccagccgtc gaccagcacg
     2341 aacagcatca gcttgaacgg aaaggagatc accagcggcg acagcatcat catgcccatc
     2401 gccatcagca cgctggagac cacgaagtcg atgatcagga acgggatgaa cagcatgaag
     2461 ccgatctgga aggcggtctt cagctcgctg gtcacgtagg ccggcaccag cagcgtgaac
     2521 ggcacgctct cgggcgattc gaaggactcg cgacccgaga gcttcgcgaa cagcgccaga
     2581 tcggactcgc gcgtctgcgc gagcatgaac tgcgacagcg gcacgcgcgc cttctcgacc
     2641 gcctcctgga agccgatgcg ctcttcgagg tacggcgtca gcgcctcgtc gtgcatgcgc
     2701 tgcagcaccg gccccatgat gaacacggtc aggaacagcg ccagcccgat cagcacctgg
     2761 ttcgacggcg tctgcatcgt gccgagcgcc tggcgaagca gcgccagcac gatgacgatg
     2821 cgcgtgaagg ccgtggtcat cagcaccagc gacggcagca gcgtcagcgc cgtcatgatc
     2881 gcgaggatct gcaggctgac cgaccactcc tgcacgccgc cggtgcccgg cgtcatcgtc
     2941 accgccggca cgcgcggctc ggcgagcgcg agcgccgtgg cggcgcacat cgcgacgagt
     3001 ccgccgccga gcgcgagcgc cctacggatg cggcggtcca ccgccgtcct cccgtgcccg
     3061 gtggtaatcg gcgagcgccg agcggaagtc gaaacgccgg cgcgggctgt cctcgccgtc
     3121 cgtctcgccc gcgcccgcgc cgtgcacggc caggcggtcg agcacgtgca gcgtcgtgac
     3181 gctgccgggg gtcacgccga gcagcacgcg ctcgccgtgc acctcgacca gcacgatgcg
     3241 ctcgcggttg ccgacgttga gcccgccgag gatcgtgatc ggtccggagc tgcgcctgag
     3301 cgtcggcacc tgccgcgcca gccacgcgag cgccaggatc accgcgatca cgaccagcag
     3361 cttgatgccg aggttgacca gcgaactcac gtcgaaggcc ggcgccgccg gcggctgggt
     3421 ttcgggggct gcagcggcgc gcccggcagc gaacaggctg gccagtgccg gcagcctcca
     3481 cgcggcccgc ggggtccggt gtcgcgtttc cgtcatcagc gcagcctctt gatgcgttcc
     3541 tcgggactga cgatctcgac gatgcgcacg ccgaagcggt cgttgacgac gacgacctcg
     3601 ccgcgcgcca ccagacagcc gttgacgagg atgtccagcg gttcgccggc cgggcggtcg
     3661 aggcgcacga cgttgccctg gctgagcgcc agcagctcgc ggatcgacag ccgcgtgcgg
     3721 cccagctcca tcgacagcgt gatcgggatg tcgagcacga catcgagatt ggccgcccgc
     3781 ccgttcgcgg acggaaccgg gtcgccggcc gtgtcgaaat tcgtgccgaa tcccccgctg
     3841 ttgcgggttg cggcagccgg cttcggcggc ggggcggccg ggcgggccgc cggcttcggg
     3901 ggcggggcgg agctcgcggc cgcaggttcc gtggcggcgg cgggggatgc gctgtcttcg
     3961 gcgccttcgg gccggtcctc gggttcgctc atggcttcct ctccgctttg gccggggcct
     4021 tgtcccggcc gacgggtttc agggcgcgtt cggcggatgg cagttcgaat gtttcgggca
     4081 gcgacaccag ccagcggtcg atctgcagcg cccgccagcc ctgggtgtcg ccgaaatggc
     4141 cggcaagcag gcgcacgccg tcgacttcga gccgtgcctg atcgggcagg tcgaccggga
     4201 tcgtgtcgcc gacctgcagg ttcagcaggt cgttgacgct gacctccagc tcggccagca
     4261 cgcagctcac ctgcacctgc gcatcgcgca cgccctcctc cagcagccgc tgcagacgct
     4321 cgtcgcgcag cggatgctcg cgtgcgagcg ccgcgagcag cgtgccacgc accggctcga
     4381 acatcgacga cggcagcgcg acgcgcaagg tgccgagcgt gccgtcgagc atcaggtcga
     4441 tgccgaacac gatcagcagc tcgccgggat ggatggccgt gaccagatgc gggctgatct
     4501 cggaggcgtg ctgcgtccag cgcaccggca ggtagggctg ccaggccgag accagcgcct
     4561 ttgcgcacag cgcgttcagg cgctgcacca tgcgcagctc cgcggccgtg aactccaggc
     4621 ggtcggtctc gctgtactgc gtgccctgac cgccgtacca ggccccgacc agcgagaaca
     4681 ccagcgggtg atcgaacagg atcagcccgg gaccggccat cggcgacagg ccgatgacgt
     4741 tgacgctgca cggcggatgc aggcgcgcga cgaagtcctg gtagggctcg gcgtgcacct
     4801 cgaccggcgt cagcggcagg tcgcgacgca gcagctcgta gaagccgaga ccgagctgat
     4861 gcacgaagcg catctgcacg gtgttcagcg ccggctggat gcaggcgatg acgttctcgg
     4921 ggctggcgag gtcgtaggcg agcacttcgc cggcggactg gccgcggccg accacgacgc
     4981 ggccgttgcc gacgccttcc atcagcgcgt cgagttcgtc ctgcgaaagc agatcgcggt
     5041 tcggcacggc ggcggactct ccggaacggg gcggcgatgc aggcgaggcc tactgcatca
     5101 cgaagttggt gaagtacacg gcttcgatgc cgggcgtgcc ggtttcggcg atcaggatct
     5161 tctggatttc ctccagcgtc ttctgctgca gcttctcctt gccggccggc gtgccgagtt
     5221 cggcgaaggt ctggccggag aacagcagga tcagccggta gcggatctgc gggtcattct
     5281 ccttgacctt gtcgagcgcc ttctggtcgc gggccatgat cgagacgctg atctgcacgt
     5341 agcgcagcac gccctgatcg tcgaagttga cgacgaaagg cggatcgagc ggctggtact
     5401 tggccggccc ggccgacttc ggcaccgcga ccggcacggc catcggcgcg ccatggccgc
     5461 cggccgccac cgcggaacca tcatcgccgc cgtgcccgag aaacttgttg agggcgccgg
     5521 tgaagaacag cgtggcgccc accgccagga tcagcaggat gagcacgagc aggatgatga
     5581 tgatcagctt gcccttgccg cctccgccac caccggcggc tgccgcttcc gcgggcttgg
     5641 cttcttcttt ctttgccatg ggtgcagtcc ctgagggttc gaacgtgagg cctgccagag
     5701 caatttggaa gccaactcga caaggtttcg ccgtggcctt tccaatcagt agcttgtccg
     5761 ccggcatcac gtcagcggga ttcccggacg cggcaccggg ccggcagggt gacggaaatc
     5821 cgtcgccgga tgagcatcag ccgccagcaa ccatagccgc tgtccccggc ccgcggcgcc
     5881 ccggcgccgg ctgcaatgcc gcgcaatgcg gatgccatcc gcagccggca ccgccgcccc
     5941 ggtggccgct gtacccgccg ccggcttacg atccccgccc ctgtcccccg ccggagtcct
     6001 gacatgtcct cgaccgaaat cgccgctgcc gcgccggggc gcggctgggg acgcgcgcgg
     6061 cgggtcgcgg cggccttcgt cgcgcgcaac atcgagttcg tgcgcgatcg caccgcgctc
     6121 ggctggaatc tggtgatgcc ggtgctgatc gtgctcggct tcgcgttcgg ctactccggc
     6181 gagccggcgc cgcagtacaa gatcggcctg ctcaatgacg gcgccgcgca cccggccttc
     6241 ctcgataccc ggcacgtgca gttcgtgccc tacgccgagg ccgacaccga agccgcgatc
     6301 acgcgcgtcg agcgccacca gctcgacctg ctcatcgacc tgcgcacgca gcactactgg
     6361 gtcaacgagg aatcggcgaa gggctacctc gccgaacggc tgctggccgg cagcgacggc
     6421 ggttccggct tccggcgcga ggcggtgcgc ggccgggtga tccgctacgt cgactggctg
     6481 atccccggcg tgctgtcgat gaacatgatg tggagcgcgc tgtacggcgt cggctacgtg
     6541 atcgtgcgct accgcaagaa cggcgtgctc aagcgcctga aggcgacgcc gctgagtgcc
     6601 gtcgaattcc tcgccgcgca gatcggctcg cggctgacgc tggtgctgct ggtcagcacg
     6661 ctggtgttca ccggcacgca tctgctcatc ggctacccga tgtacggcag ttatctgcac
     6721 ctgctgctgg tgttcgcgct cggcgcgagc agcctgatcg gcatcggtct ggtgatcgcc
     6781 gcgcgcctgc gcaccgagga gatggccgac ggcctgctga acctgatctc gtggccgatg
     6841 atgtttttat ccggcatctg gttctcgctc gacggcatgc acccgtgggt gcagaagctc
     6901 gcgctgatct tcccgctgac gcacgtcacg accgcggcgc gcgcggtgat gatcgacggc
     6961 gccggcctcg cgaccatcgc cccgcagctc ggcgtgctcg ccggcttcac gctg
//
LOCUS       DAOQIE010000212         6994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5705_length_6994_cov_8.153624, whole genome shotgun sequence.
ACCESSION   DAOQIE010000212 DAOQIE010000000
VERSION     DAOQIE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5705_length_6994_cov_8.153624"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1924)
                     /locus_tag="PK144_12890"
     CDS             complement(146..1924)
                     /locus_tag="PK144_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNF66667.1"
                     /translation="MQPTHLLGDRLRRTGVYAVLQALVLMAVALSAVTFLALQLQAAR
                     TAAQALRNHLEADMMHDALRGDVLMARTPGFTPAEVQEELRDHATRFRDLLAANRSLD
                     VPKVLADAFAAVAPQAERYIASAEQEVAAVLGGQSGDLPGFITQFNHLEDAMERVSEA
                     VSANAADTEAAGNRAALLAAGLLLLVGIGTLSAALLGGRRLARDVSMPLLRLADGMRR
                     LAAGERGLDRADCARPDEIGEMARALLVFEETAVRAEALANARAAEHAAVEAERERVR
                     LADEQARLAAEAEREQRRQVEAEAREQRQRERERRAERVAQLAADFERQSNSSLQAVT
                     NAAQDLTRTAQGLAGGAERTQQRAEAVGKASSGAADSVRRMAEASAQMAVAVSQIAAQ
                     AGQSAQTTRLAVDEAAATQRTVSGLSDAARTIGRIVDLINTIADQTNLLALNATIESA
                     RAGEAGKGFAVVANEVKALAHQTAEATDEISEQIGTVQQIAGESVLAITRIADTIGGL
                     SRIADSITSAIEAQQHAMQTIDEQVQQAMAGTQAVGADIGAVNLAAADTGTAAAQVLG
                     AAGRLGEQATTLRSEMARFLGRLNAE"
     gene            complement(2072..2422)
                     /locus_tag="PK144_12895"
     CDS             complement(2072..2422)
                     /locus_tag="PK144_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNF66668.1"
                     /translation="MNDAAQPSADVTPEQVEVEQQIVRALKTVYDPEIPVDIWELGLV
                     YGVNVNLGDRSAHIYMTLTAPACPVAGSMPEWVENAVRGVDGIDDVTVELVWDPPWTQ
                     EMISARARLELNLL"
     gene            complement(2440..2883)
                     /locus_tag="PK144_12900"
     CDS             complement(2440..2883)
                     /locus_tag="PK144_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNF66669.1"
                     /translation="MSDLRELYQEVILDHNKRPRNFRVIDPASHHAEGFNPLCGDRLT
                     VFIDVADGVIRDLAFQGQGCAISTASASLMTEALKGRPVAEVEPLFVKFHDLVTTDGP
                     TPPGLGKLGVLAGVREFPSRVKCATLAWHALKAALDHRSAPVATE"
     gene            complement(2927..4180)
                     /locus_tag="PK144_12905"
     CDS             complement(2927..4180)
                     /locus_tag="PK144_12905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNF66670.1"
                     /translation="MSAPIVTPVPAAAFDVEAVRAQFPVLAQQIRGKHLVYLDSGASA
                     QKPEVVVQAVADCYRGYYSNIHRGVHALSERSTTAFERAREQVARFVNAPSPREIVFL
                     RSTTEAINLVANSFLEPQLGPDDEILVTEMEHHSNIVPWQLVADRYGAKVVAAPVTDR
                     GELDLDGLTRLISGRTKLVAVTHVSNVLGTINPIADIVRLAHAQGVPVLVDGAQGVPH
                     LAVDVQALGCDFYAFSAHKVYGPSGVGALWARAELLAAMPPYQGGGGMIRRVSFSGTT
                     FADPPERFEAGTPDVAGVIGFGAAVEWTRALGLDAIAAHEHELTQYAMARLADIPGLR
                     LIGTAADKAGVVSFVMDGVHPHDIGTILDGEGVAVRVGHHCAQPLMERFAVPATARAS
                     FGVYNTSADVDALAAALDKVRHWFG"
     gene            complement(4177..5472)
                     /gene="sufD"
                     /locus_tag="PK144_12910"
     CDS             complement(4177..5472)
                     /gene="sufD"
                     /locus_tag="PK144_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNF66671.1"
                     /translation="MNPVAVTAVTDPRAHWLAAATETAAGLPPELALPELRAAARTRF
                     ERLGLPSRTLEAWRTTDIAPLRGLEPRRAGTGRAPDAGWLAGLPAGPRVVLVDGRVHA
                     IETGELPAGVSVLGFAEAARRLPQVLAALDTLPGLDAHAFAALNSALFEDGVLVHVAA
                     GARLDALTLAHWSSGDGTAVHPRLLLVLEDGAGAGLLEWQGGQGTYLSTALTEVRLGR
                     GAQLALSTVQDESAEAFQLGGLRAQLAADAKFSLRRWVGGGRIGRMDLALTLEGEGAA
                     AELDGLVLAGGTQLLDHHLELRHAVARCTSRQTFRGVLAGKAKHVFDGLIRVDPDAQQ
                     TDAQQQCRNLLLSKQALTVAIPRLEILADDVKCAHGATVGALDEDALFYLRARGIGLA
                     EARTLLVRAFAGEFLDALTDEAMRAALETRLDAWMEVAA"
     gene            complement(5496..6266)
                     /gene="sufC"
                     /locus_tag="PK144_12915"
     CDS             complement(5496..6266)
                     /gene="sufC"
                     /locus_tag="PK144_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNF66672.1"
                     /translation="MLEIRNLHAALEGREILKGIDLSIRAGEVHAIMGPNGSGKSTLA
                     NVLAGRPGYEASAGEVLFDGQDLLALAAEERAREGLFLAFQYPVEIPGVNNLYFLKAS
                     INAIRKHRGQEELDALEFLERVQEKAGLVKMDEAFLKRPVNEGFSGGEKKRNEILQMA
                     VLEPKFAVLDETDSGLDIDALKVVADGVNALRSPERGFLVITHYQRLLDYIVPDVVHV
                     LVDGRIVRSGGPELAHELEAQGYTLYGREAGASRRAQA"
     gene            complement(6353..>6994)
                     /locus_tag="PK144_12920"
     CDS             complement(6353..>6994)
                     /locus_tag="PK144_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNF66673.1"
                     /translation="VQNWYPGDKEGRGGIYNFVTKRGLCRGARSKISWTQVETGSAIT
                     WKYPSCILRGDDSVGEFYSVALTRDRMQADTGTKMIHMGKRTRSTIVSKGISAGHAQN
                     AYRGLVKILPTADGARNHSQCDSLLMGDQCGAHTFPYVEVQNPTAQVEHEATTSRVSD
                     DQIFYLNQRGIGTEDAVSLIVNGFCKEVFKELPMEFAVEAQKLLELKLEGSVG"
BASE COUNT          973 a         2452 c         2490 g         1079 t
ORIGIN      
        1 gccggcgtct acccctctcc cccggcccct ctcccgcaag gggtgagggg agattgacgc
       61 ccgggcgttg atcggtcgat ccatccggcc cgcggaagcg ctgacttgtg tagataccaa
      121 tgctgcacga cggcgggatt cggcgtcatt cggcgttgag gcgtccgagg aagcgcgcca
      181 tctcgctgcg cagcgtcgtc gcctgctccc cgagccggcc ggcggcaccg agcacctggg
      241 cggcagccgt gccggtatcg gccgcggcga ggttcaccgc cccgatgtcg gccccgaccg
      301 cctgcgtgcc ggccatggcc tgctgcacct gctcgtcgat ggtctgcatc gcgtgctgct
      361 gggcctcgat ggccgaggtg atgctgtcgg cgatgcgtga caggccgccg atggtgtcgg
      421 cgatgcgcgt gatcgccagc accgactcgc cggcgatctg ctgcacggta ccgatctgct
      481 cggagatctc gtcggtggct tcggcggtct ggtgggcgag cgccttgacc tcgttggcga
      541 ccactgcaaa gcccttgccg gcctcgccgg cgcgggccga ctcgatcgtc gcgttcagcg
      601 ccagcagatt ggtctggtcg gcgatcgtgt tgatcaggtc gacgatgcgg ccgatcgtgc
      661 gcgccgcgtc actgagcccg gaaaccgtac gctgggttgc ggcggcctcg tcgacggcga
      721 ggcgtgtggt ctgggcggac tgtccggcct gcgcggcgat ctggctgacg gcgaccgcca
      781 tctgcgcact ggcctcggcc atgcgccgga cgctgtcggc cgcccccgag gaagccttgc
      841 cgaccgcttc ggcgcgctgc tgcgtgcgtt cggcgccgcc ggccagcccc tgcgcggtgc
      901 gggtcaggtc ctgggccgcg ttcgtgaccg cctgcaggct gctgttcgac tggcgctcga
      961 agtccgcggc cagctgcgcg acgcgctcgg cccgccgctc gcgctcgcgc tggcgctgtt
     1021 cgcgggcttc ggcctcgacc tggcggcgct gctcgcgctc ggcttcggca gccaggcgcg
     1081 cctgctcatc ggcgaggcgc acgcgctcgc gctcggcctc gaccgccgca tgttcggccg
     1141 cccgggcatt ggccagcgcc tcggcgcgca ccgcggtttc ctcgaacacc agcagcgccc
     1201 gcgccatttc gccgatttca tcggggcgcg cgcagtccgc gcgatcgagc ccgcgctcgc
     1261 cggcagcgag ccggcgcatg ccgtcggcca ggcgcagcag cggcatgctc acgtcacgcg
     1321 ccagccgccg cccgccgagc agggcggcac tgagcgtgcc gatcccgacc agcagcagca
     1381 ggccggccgc gagcagggcc gcccggttgc cggcggcttc ggtgtcggcg gcattggcgc
     1441 tgaccgcctc gctgacgcgc tccatcgcat cttcaaggtg gttgaactgc gtgatgaatc
     1501 ccggcagatc gccggactgg ccgccgagca cggcggcgac ctcctgctcg gcgctggcga
     1561 tgtagcgctc ggcctgcggc gcgacggcag cgaacgcgtc ggccagcacc ttcggcacat
     1621 cgaggctgcg gttggcggcc agcaggtccc ggaaacgcgt ggcgtgatcg cgcagttcct
     1681 cctgcacctc ggccggcgtg aaccccggcg tgcgcgccat cagcacgtcg ccgcgcagcg
     1741 cgtcgtgcat catgtcggct tcgaggtggt tgcgcagcgc ctgcgcggcg gtgcgcgccg
     1801 cctgcagctg cagcgcaagg aaggtgaccg cggacaacgc gacggccatc agcacgagcg
     1861 cctgcagcac ggcatagacg ccggtcctgc gcaggcgatc gccgagcaag tgggtgggct
     1921 gcatcggagt ctccgcaatt ccgtggacga gcctcgtatg cccgacaggc atcggcagga
     1981 atcggcaaga ctgaagccgg cagacgcgac ggcgctgcac ggtgcgcagc cgccgccgag
     2041 ccggtcacgc ggcgccggca gcggcctgcg gctagagcag gttcagctcc agccgtgccc
     2101 gcgccgagat catctcctgc gtccacggcg ggtcccagac cagctcgacg gtcacgtcgt
     2161 cgatgccgtc gacgccgcgc acggcgttct cgacccactc gggcatcgag ccggccaccg
     2221 ggcaggccgg ggcggtcagc gtcatgtaga tgtgcgcgct gcgatcgccg aggttgacgt
     2281 tgacgccgta gacgaggccg agctcccaga tgtcgaccgg gatctcgggg tcgtagacgg
     2341 tcttcagcgc cctgacgatc tgctgttcga cctcgacctg ttcgggggtc acgtctgcgg
     2401 atggctgggc ggcatcgttc atcgcggact ctccgggctt cattcggtcg ccaccggcgc
     2461 gctgcggtga tcgagcgcgg ccttcaatgc gtgccaggcg agcgtcgcgc acttgacgcg
     2521 gctcgggaac tcgcgcacgc cggcgagcac gccgagcttg ccgaggccgg gtggcgtcgg
     2581 gccgtcggtg gtgacgagat catgaaactt cacgaacagc ggctcgacct cggcgaccgg
     2641 cctgcccttc agggcctcgg tcatcagcga ggccgacgcg gtcgagatcg cgcagccctg
     2701 gccctggaag gcgaggtcgc ggatcacgcc gtcggccacg tcgatgaaca cggtcagccg
     2761 gtcgccgcag agcgggttga agccctcggc gtgatgcgag gccgggtcga tcacgcggaa
     2821 gttccggggg cgcttgttgt gatcgaggat cacttcctgg tacagctcgc gcaggtcact
     2881 catcgcaaca ccttgaagga attcggtttc tgaggtccga ggtgtttcag ccgaaccagt
     2941 ggcggacctt gtcgagcgcg gcggcgagcg cgtcgacatc ggcggacgtg ttgtagacgc
     3001 cgaacgaggc gcgggcggtc gccggcaccg cgaagcgctc catcagcggc tgcgcgcagt
     3061 ggtgaccgac gcgcacggcg acgccttcgc cgtcgaggat cgtgccgatg tcgtgcggat
     3121 gcacgccgtc catcacgaag gacacgaccc cggccttgtc cgctgcggtg ccgatcaggc
     3181 gcaggcccgg gatgtcggca agacgcgcca tcgcgtactg cgtcagttcg tgctcgtgcg
     3241 cggcgatggc atcgagcccg agcgcgcgcg tccactcgac cgcggcgccg aagccgatga
     3301 cgccggcgac atccggcgtg ccggcctcga agcgctccgg cggatcggcg aaggtcgtgc
     3361 ccgagaagct cacgcgccgg atcatgccgc cgccgccctg atacggtggc atcgccgcca
     3421 gcagctcggc acgcgcccac agcgcgccga cgccgctcgg cccgtagacc ttgtgcgccg
     3481 agaacgcata gaagtcgcag ccgagggcct gcacgtcgac ggccagatgc ggcacgccct
     3541 gcgcgccgtc gaccagcacc ggcacgccct gcgcgtgcgc cagacgcacg atgtcggcga
     3601 tcgggttgat cgtgccgagc acgttcgaga cgtgcgtgac cgcaacgagc ttcgtgcggc
     3661 cgctgatcag ccgcgtcagt ccgtcgagat cgagctcgcc gcggtcggtg accggagcgg
     3721 cgacgacctt cgcgccatag cggtcggcca ccagctgcca gggcacgatg ttggagtggt
     3781 gctccatctc ggtgacgagg atctcgtcat cgggcccgag ctgcggctcc agaaaactgt
     3841 tcgcgaccag gttgatcgcc tcggtcgtgc tgcgcaggaa cacgatctcg cgcggcgacg
     3901 gcgcgttgac gaagcgtgca acctgctcgc gcgcgcgctc gaaggcggtg gtcgagcgct
     3961 cggacagcgc atgcacgccg cggtggatgt tcgagtagta gccgcggtag cagtcggcga
     4021 ccgcctgcac gacgacctcg ggcttctgcg ccgaggcgcc gctgtcgagg tatacgagat
     4081 gcttgccgcg gatctgctgt gcgagcaccg ggaactgcgc gcgcacggcc tcgacatcga
     4141 aggccgctgc gggtactggg gtcacgatcg gggcgctcat gcggccacct ccatccaggc
     4201 atcgagtcgg gtttcgagcg cggcgcgcat ggcctcgtcg gtcagcgcgt cgaggaattc
     4261 accggcgaag gcgcgcacca gcagcgtgcg cgcctcggcc agaccgatgc cgcgcgcgcg
     4321 caggtagaac agcgcatcct catcgagcgc gccgaccgtc gcgccgtgcg cgcacttcac
     4381 gtcgtcggcg aggatttcga ggcgcgggat cgcaaccgtc agcgcctgct tcgacagcag
     4441 caggttgcgg cactgctgct gcgcgtcggt ctgctgcgca tccggatcga cgcggatcag
     4501 gccgtcgaac acgtgcttcg ccttgccggc gagcacgccg cggaaggtct ggcgactcgt
     4561 gcagcgcgcc acggcatgac gcagttcgag gtggtgatcg agcagctgcg tgccgccggc
     4621 cagcaccaga ccgtcgagtt cggccgcggc gccctcgcct tcgagcgtga gcgcgagatc
     4681 catccgcccg atgcgcccgc cgccgaccca gcggcgcagc gaaaacttcg cgtcggcggc
     4741 gagctgcgcg cgcaggccgc cgagctggaa ggcctcggcg gattcatcct gcaccgtgct
     4801 cagcgccagc tgcgcgccgc gtccgagccg gacctcggtc agcgccgtcg acagataggt
     4861 cccctgcccg ccctgccact ccagcagccc ggccccggct ccgtcttcga gcaccagcag
     4921 caggcgcgga tgcacggccg tgccgtcacc gctgctccag tgcgcgagcg tcagcgcgtc
     4981 gagccgcgca ccggcggcga cgtgcacgag cacgccgtcc tcgaacagcg ccgagttgag
     5041 cgcggcgaag gcatgcgcat cgaggcccgg cagcgtgtcg agtgcggcca gcacctgcgg
     5101 cagccggcga gcggcctcgg cgaagccgag cacgctgacg cctgccggca gttcaccggt
     5161 ttcgatcgcg tgcacccggc catcgaccag cacgacgcgc ggcccggccg gcagcccggc
     5221 cagccagccg gcgtcgggcg cacgcccggt gccggcacgc cgcggttcga gcccgcgcag
     5281 cggcgcgatg tcggtcgtgc gccaggcctc cagcgtgcgc gacggcaggc cgagacgctc
     5341 gaaacgcgtg cgcgccgcgg cgcgcagttc gggcagcgcc agctccggcg gcaggccggc
     5401 ggcggtctcg gtcgccgcgg ccagccagtg cgcgcgcgga tcggtcacgg cagtgacggc
     5461 tacgggattc acggtactca cgccgcccac cccgctcagg cctgcgcccg gcgactggcg
     5521 ccggcttcgc ggccgtacag cgtgtagccc tgggcttcga gttcgtgcgc aagctccggc
     5581 ccgccggagc gcacgatgcg gccatcgacc agcacgtgca cgacatcggg cacgatgtag
     5641 tcgagcaggc gctggtagtg cgtgatcacc agaaagccgc gttcggggct gcgcagcgcg
     5701 ttgacgccat cggcgacgac cttcagcgcg tcgatgtcga ggccggagtc ggtctcgtcg
     5761 agcaccgcga acttcggctc cagcaccgcc atctgcagga tttcgttgcg cttcttctcg
     5821 ccgcccgaga agccttcgtt gaccgggcgc ttcaggaagg cttcgtccat cttcacgagc
     5881 ccggccttct cctgcacgcg ttcgaggaat tcgagcgcgt cgagctcctc ctggccgcgg
     5941 tgcttgcgga tggcgttgat cgaagccttc aggaagtaca ggttgttgac gcccggaatc
     6001 tcgaccgggt actggaaggc gaggaacagc ccctcgcgtg cgcgctcctc ggcggccagc
     6061 gccagcaggt cctgaccgtc gaacagcacc tcgccggcac tggcctcgta gccgggccgt
     6121 ccggccagca cgttggcgag cgtgctcttg cccgagccgt tcgggcccat gatcgcgtgg
     6181 acttcgccgg cgcggatgct caggtcgatg cccttgagga tttcgcggcc ttccagcgcg
     6241 gcgtgcagat tgcggatttc cagcatggtt cgttccggtt tccggggagc cggcgcaggg
     6301 ccggtcgatc gggtcagtag aggaggaatc gggtgcgggc gcgcgacggc gatcagccga
     6361 cgctgccttc gagcttcagt tcgagcagct tctgcgcctc gacggcgaac tccatcggca
     6421 gctccttgaa cacttccttg cagaagccgt tgacgatcag gctgaccgcg tcctcggtgc
     6481 cgatgccgcg ctggttcagg tagaagatct ggtcgtcgga gacgcgcgag gtggtcgcct
     6541 cgtgctcgac ctgggccgtc gggttctgca cctcgacgta cgggaaggtg tgcgcaccgc
     6601 actggtcgcc catcagcagc gagtcgcact gcgaatggtt gcgggcgccg tcggcggtcg
     6661 ggaggatctt cacgaggccg cggtaggcgt tctgcgcatg accggccgag atgcccttgg
     6721 agacgatggt cgagcgcgtg cgcttgccca tgtggatcat cttggtgccg gtgtcggcct
     6781 gcatgcggtc gcgcgtcagc gccaccgagt agaactcgcc gaccgaatcg tcaccgcgca
     6841 ggatgcagct cgggtacttc caggtgatcg ccgagccggt ctcgacctgc gtccaggaaa
     6901 tcttcgagcg cgcgcccctg cacaggccgc gcttggtcac gaagttgtag atgccgccgc
     6961 ggccttcctt gtcgcccgga taccagttct gcac
//
LOCUS       DAOQIE010000213         6985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5720_length_6985_cov_7.296825, whole genome shotgun sequence.
ACCESSION   DAOQIE010000213 DAOQIE010000000
VERSION     DAOQIE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5720_length_6985_cov_7.296825"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..2092)
                     /gene="recN"
                     /locus_tag="PK144_12925"
     CDS             complement(422..2092)
                     /gene="recN"
                     /locus_tag="PK144_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNF66674.1"
                     /translation="MLTQLSIRDYAIVESLELEFGPGMTTVTGETGAGKSIMVDALGL
                     LLGDRSDAAAVRTGTERAEIAARFDVERLPGAQDWLAERELDLETHECVLRRTIGRDG
                     RSRAWINGSPLPLASLRELGERLVDIHGQHEHQSLMQRDAQRALLDAHAGLKTELTAL
                     GAAFQHWARLRRRIDELAQRGAERDARLDMLRYQVRELEDLALGADELPQLEEEHARL
                     ANAGRLLDTCRQVLDALYEGEPVSAQGLLGTAADQLGELAGIDAALAPLTELVGSALI
                     QVQEAADGLRRYLDRLDLDPRRLGWLETRLGEISALARKHRQRPEDLPGLLDALRAEL
                     DELEHGELRLDELTQELAKAETAYRRIADQVGAARAAAAVTLGEQVGAAMHELGMPGG
                     RFAVELQPLERPTPAGLQQIEFMVSANPGQPLRPLAKVASGGELARISLALQVITAHG
                     AQIPTLIFDEVDTGIGGGVAEIVGRRLRELGSGRQVLCVTHLPQVAAQAHRHLQVHKQ
                     TDGESTATRVIALDAEARVQEIARMLGGIDITARTLAHAREMIARGQA"
     gene            complement(2171..3064)
                     /locus_tag="PK144_12930"
     CDS             complement(2171..3064)
                     /locus_tag="PK144_12930"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNF66675.1"
                     /translation="MPYLFSTIGLIGKYNDASVAPTLDQIAGWLRQAGRRVLLDEQSA
                     AHVTVRGPEVATRRLIGRQADLAIVVGGDGTLLDAARALVDFQVPIVGVNLGRLGFLT
                     DVSPNEIPAGLDEILQGRFREELRLMLHAQVLRGEQTLSEAMALNDVVVHKRDVARMV
                     ELDTFLDGQLLNAYRSDGLIISTPTGSTAYALSCGGPILHPALEALLVAPICPHTLTH
                     RPIVVGADAQIEICVRNTTHTQITCDGQISLPIEPGDRVVVRQKRRRMRLLHPVGHDY
                     FELLRAKLRWGVNPEGATSNR"
     gene            3214..4275
                     /gene="hrcA"
                     /locus_tag="PK144_12935"
     CDS             3214..4275
                     /gene="hrcA"
                     /locus_tag="PK144_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNF66676.1"
                     /translation="MARQPVPASQLSDRAQLLLKALVQRYIEDGQPVGSRTLAKNTGM
                     DLSPATIRNVMSDLEELGFLRAPHTSAGRVPTVRGYRFFVDTLMQVRPLENAAVQSME
                     RELRGAHSTHGLVQSASTLLSGLSHMAGIVMIPRRDSVTLRQIEFLPLSERRVLAIIV
                     VNEREVQNRILHVSRDYTRTELTEAANYLNRLFAGHDLQQVRAQLLAELQAAELEAND
                     LLQRVAEMAEQALSPEARELPDDFVLAGQTNLMGFSELADVQRLRGLFEAFTHKRDLL
                     GLFDQCLRADGVQIYIGDESGYEVLDECSVIAAPYGCPGQTLGVLGIIGPTRMAYERV
                     VPLVDVTAKLLSAALNSQA"
     gene            4396..4971
                     /gene="grpE"
                     /locus_tag="PK144_12940"
     CDS             4396..4971
                     /gene="grpE"
                     /locus_tag="PK144_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNF66677.1"
                     /translation="MSQEDITATPNPAAAAEAQPAPAAHEELPVATPEQELENLRAEL
                     AAVSAKAEAHWNEFLAARAEMDNLRRRSERDLANAHKFALEKFFAELLPVRDGLEMGI
                     QAAVEGADFAKLREGSEMTLRMLAAAMAKFGLVELDPAGQKFNPEQHEALAMAPVPGV
                     EPNHVVQVVQKGYVLNERLVRPAKVIVAKAP"
     gene            5133..>6985
                     /gene="dnaK"
                     /locus_tag="PK144_12945"
     CDS             5133..>6985
                     /gene="dnaK"
                     /locus_tag="PK144_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNF66678.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGKPRVIENSEGDRTTPSIVAFLE
                     DGEITVGQPAKRQAVTNPQNTLFAVKRLIGRRFTDAEVQKDIDLVPYKIVKNSNNDAW
                     VEARGKAMAPPEISARVLMKMKKTAEDYLGEPITEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKSGGKDRKIAVYDLGGGTFDISVIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDKRVIDYLVEQFQKDTGINLASDPLALQRLKESAEKAKIELSS
                     AQQTEINLPYITADASGPKHLNLKLTRAKLESLVEDLIEKTIIPCRTALKDAGVSVGD
                     IDDVLLVGGQTRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGGVLGGTVKDVLL
                     LDVTPLSLGIETLGGVMTKLIEKNTTIPTKQTQTFSTADDNQTAVTVHVLQGEREQAH
                     ANKSLGKFDLQDIPLAPRGVPQIEVTFNIDANGILHVSAKDKATGKEQSIVIKASSGL
                     NEDEIQRMVKDAEAHAEEDRKFHELVNARNGADNLIHASEKTLKDLGEKVEPAERAAL
                     ESAISELRTAAKGDNKDVITRKTEALGELSSKLATRLYSAEAGAEGAAGAGA"
BASE COUNT         1132 a         2413 c         2446 g          994 t
ORIGIN      
        1 tgcatgtcag caagccgtcg accggcggtt cctctgcagg ctttcggttt cggggagcag
       61 gccggggcgc gtcggcaatg atcaccggca gacaccggac aggcggcggc ccgggctgcc
      121 tgcctgtcga tcggcaaggc agaagccgcc cttccgggct ggatatcgac cgacacccct
      181 gcaactgtag gtgccgggaa acagcttccg gaccggttct gcgggccttc atggcccagc
      241 cggcgacagc aatgcctgcg cgtgccagca ttgctgtcgc ctgtctgtac attaattcat
      301 ccgaatgtta ataacattaa attaattgtg gtaaacagca ggagcggcgg cgcagttccg
      361 cgccaccggt gcggagctgc gccaccgtcg atgccgcgcc ggtggcggcg cgggcggggt
      421 ttcaggcctg accgcgggcg atcatctcgc gcgcgtgggc gagcgtgcgg gcggtgatgt
      481 cgatgccgcc gagcatgcgc gcgatctcct gcacgcgggc ctcggcatcg agcgcgatga
      541 cgcgggtcgc ggtgctctcg ccatccgtct gcttgtgcac ctgcagatgg cggtgcgcct
      601 gcgcggcgac ctgcggcagg tgcgtgacgc agagcacctg ccggccgctg ccgagttcgc
      661 gcagccgccg gccgacgatc tcggcgacgc cgccgccgat gccggtgtcg acctcatcga
      721 agatcagcgt cgggatctgc gcgccgtgcg cggtgatcac ctgcagcgcc agcgagatgc
      781 gcgcgagctc gccgccggag gcgaccttcg cgagcgggcg cagcggctgg ccggggttgg
      841 cgctgaccat gaactcgatc tgctgcagcc cggccggcgt cgggcgttcg agcggctgca
      901 gctcgaccgc gaagcgcccg cccggcatgc cgagctcgtg catcgccgcg ccgacctgct
      961 cgccgagcgt caccgccgcc gccgcgcgtg cggcgccgac ctgatcggcg atgcgccggt
     1021 aggcggtctc ggccttcgcc agctcctgcg tcagctcgtc gaggcgcagc tcgccgtgtt
     1081 cgagttcgtc gagttcggcg cgcagcgcat cgagcaggcc gggcaggtcc tcggggcgct
     1141 ggcggtgctt gcgtgccagt gcgctgatct cgccgagccg cgtttccagc cagccgagcc
     1201 ggcgcgggtc gaggtcgagc cggtcgaggt agcggcgcag tccgtcggcg gcctcctgca
     1261 cctggatcag cgccgagccg accagctcgg tgagcggcgc gagcgcggca tcgatgccgg
     1321 cgagctcgcc gagctggtcg gcggccgtgc cgagcaggcc ctgcgccgag accggctcgc
     1381 cttcgtagag cgcatcgagc acctggcggc aggtgtcgag cagccgcccg gcgttggcga
     1441 ggcgggcgtg ctcctcttcg agctgcggca gttcgtcggc accgagcgcg aggtcttcga
     1501 gctcgcgcac ctggtagcgc agcatgtcga ggcgcgcatc gcgctcggcg ccacgctggg
     1561 cgagttcgtc gatgcgccgg cgcagccgcg cccagtgctg gaaggcggcg ccgagcgcgg
     1621 tgagttcggt tttgaggccg gcgtgggcgt cgagcagcgc gcgctgcgcg tcgcgctgca
     1681 tcagcgactg atgctcgtgc tggccgtgga tgtcgaccag ccgctcgccg agttcgcgca
     1741 ggctcgcgag cggcagcggc gagccgttga tccacgcgcg cgagcggccg tcgcggccga
     1801 tcgtgcgccg cagcacgcat tcatgtgttt cgaggtcgag ctcgcgctcg gcgagccagt
     1861 cctgcgcccc cggcaggcgc tcgacatcga agcgcgcggc aatctcggcg cgctcggtgc
     1921 cggtgcgcac ggctgcggcg tcgctgcggt cgccgagcag caggccgagc gcatcgacca
     1981 tgatcgactt gccggcgccg gtctcgccgg tgaccgtggt catgccgggg ccgaattcga
     2041 gttcgaggct ttcgacgatc gcgtaatcgc ggatggagag ttgggtcagc atcggcagca
     2101 gggaagcgcg ggcaggaggc gtgcgggaag cgggaagcgg ctggaaaagc ccgtggcggg
     2161 cgcgccgccg tcagcggttc gaggtcgcgc cttcggggtt gacgccccag cgcagcttgg
     2221 cgcgcagcag ctcgaagtag tcgtgaccga ccgggtgcag caggcgcatg cgccggcgct
     2281 tctggcgcac gacgacgcgg tcgcccggct cgatcggcag gctgatctgg ccgtcgcagg
     2341 tgatctgcgt gtgcgtggtg ttgcgcacgc agatctcgat ctgcgcgtcg gccccgacca
     2401 cgatcgggcg gtgcgtcagc gtgtgcgggc agatcggtgc gaccagcagc gcttcgagcg
     2461 ccgggtgcag gatcgggccg ccgcaggaca gcgcatacgc ggtcgagccg gtcggcgtcg
     2521 agatgatcag gccgtcggag cggtaggcgt tcagcagctg gccgtcgagg aaggtgtcga
     2581 gctcgaccat gcgcgccacg tcgcgcttgt gcacgacgac atcgttgagc gccatcgcct
     2641 cgctgagcgt ctgctcgccg cgcagcacct gcgcgtgcag catcaggcgc agctcctcgc
     2701 ggaagcgccc ctgcaggatc tcgtcgaggc cggccggaat ctcgttcggc gacacatcgg
     2761 tcaggaagcc gagccggccg aggttgacgc cgacgatcgg cacctggaag tcgacgagcg
     2821 cacgcgccgc gtcgagcagt gtgccgtcgc cgccgacgac gatggcgaga tccgcctgcc
     2881 ggccgatcag ccggcgcgtg gcgacctcgg ggccgcgcac cgtcacgtgc gcggcactct
     2941 gctcgtcgag cagcacgcga cggccggcct gcctgagcca gccggcgatc tggtcgagcg
     3001 tcggcgcgac gctcgcgtcg ttgtacttgc cgatgaggcc gatggtgctg aagagatagg
     3061 gcatcgtgcg ggccttccgg aagcgcgtgc gggctatgat cgcacgcacc cggtgcgccg
     3121 acctatcgcc ttgacacccc gcgccggggc tgattagctg accctgtttg gcactctacg
     3181 gccctgagtg ccaatcgccg ccggagacgc accgtggccc gtcagcccgt acccgccagc
     3241 cagttgtccg atcgcgccca gctgctgctg aaggcgctgg tgcagcgcta catcgaggac
     3301 ggccagccgg tcggctcgcg cacgctggcc aagaacaccg gcatggacct gtcgccggcg
     3361 acgatccgca acgtcatgtc ggacctcgag gaactcggct tcctgcgcgc gccgcacacc
     3421 tcggccgggc gcgtgccgac cgtgcgcggc taccgtttct tcgtcgacac gctgatgcag
     3481 gtgcgcccgc tcgagaacgc cgccgtgcag tcgatggagc gcgaactgcg cggcgcgcac
     3541 agcacgcacg gcctcgtgca gtcggcctcg acgctgctgt cggggctctc gcacatggcc
     3601 ggcatcgtga tgatcccgcg ccgtgactcg gtgacgctgc gccagatcga gttcctgccg
     3661 ctgtcggagc ggcgcgtgct ggcgatcatc gtcgtcaacg agcgtgaggt gcagaaccgc
     3721 atcctgcacg tctcgcgcga ctacacgcgc accgagctga ccgaggccgc gaactacctg
     3781 aaccgcctgt tcgccggcca cgacctgcag caggtgcgcg cgcagctgct cgccgagctg
     3841 caggccgcgg agctggaagc caacgacctg ctgcagcgtg tcgccgagat ggccgagcag
     3901 gcgctgagcc cggaggcgcg cgagctgccc gatgacttcg tgctcgccgg ccagaccaac
     3961 ctgatgggct tctcggagct ggccgacgtg cagcgcctgc gcggcctgtt cgaggccttc
     4021 acgcacaagc gagacctgct cggcctgttc gaccagtgcc tgcgcgccga cggcgtgcag
     4081 atctacatcg gcgacgaatc cggctacgaa gtcctcgacg aatgcagcgt catcgccgcg
     4141 ccctacggct gccccggcca gacgctcggc gtgctcggca tcatcgggcc gacgcgcatg
     4201 gcctatgagc gcgtcgtgcc gctcgtcgac gtgaccgcga agctgctgtc ggcggccttg
     4261 aattcccagg cctgaccccc cagataccga gcccgtatgc ctgcatcgcc gtcctgcggc
     4321 ggcgcgtgcg ggcgtccttt gctgccgaaa ccggccgtca cgcacggtcg cccgccttac
     4381 cggagagcca tccgcatgag ccaggaagac atcaccgcga ccccgaatcc cgccgccgcc
     4441 gccgaggcgc agccggcgcc ggccgcgcac gaggagctgc cggtcgcgac ccccgaacag
     4501 gaactggaga acctgcgcgc cgagctggcc gcggtcagcg ccaaggccga ggcgcactgg
     4561 aacgaattcc tcgccgcgcg cgccgagatg gacaacctgc gccggcgcag cgagcgcgat
     4621 ctggccaacg cccacaagtt cgcgctggaa aaattcttcg ccgagctgct gccggtgcgc
     4681 gacggcctgg agatgggcat ccaggccgcc gtcgagggtg ccgacttcgc gaagctgcgc
     4741 gagggcagcg agatgacgct gcgcatgctc gccgcggcga tggccaagtt cgggctggtc
     4801 gagctcgacc cggccggcca gaagttcaat cccgagcagc acgaggcgct ggcgatggcg
     4861 ccggtgcccg gcgtcgagcc gaatcacgtc gtgcaggtcg tgcagaaggg ctacgtgctc
     4921 aacgagcgcc tcgtgcgccc ggccaaggtc atcgtcgcga aggcgccgtg acggcggcat
     4981 gaacatttca tgtgattcgc gcaattcgcg tgccgcggac gcttgaagcg ccggcccgcg
     5041 tccccatttc cgcgaacagc ggcgggtcgc cgcgacccgc aagaccgaac cgcagcccca
     5101 gcctgccagc gcaccccttc agagaggaca tcatgggcaa gatcatcggc attgacctcg
     5161 gcacgaccaa ctcctgcgtc gccatcatgg aaggcggcaa gccgcgcgtc atcgagaaca
     5221 gcgagggcga ccgcaccacg ccgtcgatcg tcgccttcct cgaagacggc gagatcaccg
     5281 tcggccagcc ggccaagcgt caggccgtca ccaatccgca gaacacgctg ttcgcggtca
     5341 agcgcctgat cggccgccgc ttcaccgatg ccgaggtgca gaaggacatc gatctcgtcc
     5401 cgtacaagat cgtcaagaac tccaacaacg acgcctgggt cgaggcgcgc ggcaaggcga
     5461 tggcgccgcc cgaaatctcg gcgcgcgtgc tgatgaagat gaagaagacc gccgaggact
     5521 atctcggcga gccgatcacc gaggccgtga tcacggtgcc ggcctacttc aacgactcgc
     5581 agcgccaggc gaccaaggac gccggccgca tcgccggtct ggaggtcaag cgcatcatca
     5641 acgagccgac cgcggcggcg ctcgccttcg gcatggacaa gtccggcggc aaggaccgca
     5701 agatcgcggt ctatgacctc ggcggcggca ccttcgacat ctcggtcatc gagatcgccg
     5761 atgtcgacgg cgagaagcag ttcgaggtgc tctcgaccaa cggcgacacc ttcctcggcg
     5821 gcgaggactt cgacaagcgt gtcatcgact acctcgtcga gcagttccag aaggacaccg
     5881 gcatcaacct cgccagtgac ccgctggcgc tgcagcgcct gaaggaatcg gccgagaagg
     5941 ccaagatcga gctgtcctcg gcgcagcaga ccgagatcaa cctgccgtac atcaccgccg
     6001 atgcctcggg tccgaagcac ctgaacctga agctgacgcg cgccaagctc gaatcgctgg
     6061 tcgaggacct gatcgagaag accatcatcc cgtgccgcac ggcgctcaag gatgccggcg
     6121 tctcggtcgg cgacatcgac gacgtgctgc tggtcggcgg ccagacccgc atgccgaagg
     6181 tgcaggaggc ggtcaaggcc ttcttcggca aggagccgcg caaggacgtg aaccccgacg
     6241 aggcggtcgc cgtcggcgcg gcgatccagg gcggcgtgct cggcggcacg gtcaaggacg
     6301 tgctgctgct cgacgtgacc ccgctcagcc tcggcatcga gacgctcggc ggcgtcatga
     6361 ccaagctgat cgagaagaac accaccatcc cgaccaagca gacgcagacc ttctcgaccg
     6421 ccgacgacaa ccagacggcc gtcaccgtgc acgtgctgca gggcgagcgc gagcaggcgc
     6481 acgccaacaa gtcgctcggc aagttcgacc tgcaggacat cccgctggcc ccgcgcggcg
     6541 tgccgcagat cgaggtgacg ttcaacatcg acgccaacgg catcctgcac gtctcggcaa
     6601 aggacaaggc caccggcaag gagcagagca tcgtcatcaa ggcgagctcg ggcctcaacg
     6661 aggacgagat ccagcgcatg gtcaaggacg ccgaggcgca cgccgaggag gaccgcaagt
     6721 tccacgagct ggtcaatgcc cgtaacggcg ccgacaacct gatccacgcc tcggaaaaga
     6781 cgctgaagga cctcggcgag aaggtcgagc cggccgagcg cgccgcgctc gaatcggcaa
     6841 tctcggagct gcgcaccgcg gccaagggtg acaacaagga cgtgatcacc aggaagaccg
     6901 aggcgctcgg cgagctgtcc tcgaagctcg cgacccggct gtactcggcc gaggccggag
     6961 ccgaaggcgc cgccggtgcc ggtgc
//
LOCUS       DAOQIE010000214         6958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5757_length_6958_cov_7.093148, whole genome shotgun sequence.
ACCESSION   DAOQIE010000214 DAOQIE010000000
VERSION     DAOQIE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_6958_cov_7.093148"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="PK144_12950"
     CDS             complement(<1..1356)
                     /locus_tag="PK144_12950"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNF66679.1"
                     /translation="MADRLHAAEARPTAPACCFVAAPAATLGGAAGNRGGRTMPGKVF
                     ISYSREDADWLALATEHLGAIELEGWDDTRIGPGEDWLPAIERAMAECQFALLLVSAS
                     YLSSPFIKRTEIPALLQRRAAEGLTVIPVLVRPCAWSKVDWLARINMRPKGDKALSRL
                     GKPKAEAELAALAGEIAGRLATAAAARQREAPHVDLHHLPEGAADFFGRGADLEWLDA
                     QWQTAGAVSVAVLVAPGGTGKTSLLRRWLQRLKVNGWGGAQRVFAYSFYRQGVNDAGA
                     GTATDEPFLNAALAFFEVRCEAQASAWDRGRRLAQALDRQRALLVLDGVEPLQDAGSY
                     RLRTDGLKALLTTLADAGRQSLCVLSTRAEIADLQHYGADGAVRRHRLDNLCAADGAR
                     LLHRLGVHRIGGQTLSEDEAAQDTTYHCACQSFGGHALTLHILGLYLASRLQGDLRRL
                     DR"
     gene            1572..2249
                     /locus_tag="PK144_12955"
     CDS             1572..2249
                     /locus_tag="PK144_12955"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF66680.1"
                     /translation="MKSLSLTGLLLATLAGSTAVALPLVDQTGNLLSNGNFESGSTTP
                     TNSGSGVSTFSSLTGWNQYSNTGPVTSTWAGTPVVEGDHSARITGNTNDGLYQYNGGY
                     TGTYTLSAWVYALSGSAHLVLAWNSGSTTAIGSSSTLTGQWEYLSVTATMNGSLGGPV
                     LYGASNNADFYIDGVWINAGSTSSSPMDPGTGFNPNAISTDVPEPASAALLLGGLGLL
                     ARRRTRR"
     assembly_gap    2275..2374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2376..6323)
                     /locus_tag="PK144_12960"
     CDS             complement(2376..6323)
                     /locus_tag="PK144_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNF66681.1"
                     /translation="MRRLLHPVARHTWLWGHRIGLALLFVAALLVGLARLFLPQLADH
                     RPEIETWLGETLGAPVTLGELHAGWYGWGPALEVRELRVRDPAGGAVLLAFERAAVGL
                     DPLASLRQRRPVVSALELAGVRVRLETGPDGALRLQAESGGAPVPLESLLAWLAQVRR
                     FDLRDGELQIVDPQAPAQTLVYRDLRLTLHGGAGERRAGVALRTPGDGEVQAVLALHG
                     AADTPAIWRGEFWLKAARLDLARLPFAARPASGTVDAELWGDWADGTVTRLLGRLDAD
                     RLQPAALPALPGLAPLLAGLPRLGLRFDWRRDDVATNPDGPWQAALDYSAHDADGARI
                     EGGTLGVQRAADGQLELGATDLPVDALLRLAAAQAGPEAGGWLARLQPSLRIPQARLR
                     FDPAHPDAWAAAALVEDLRVHADGAVPGIDRLDGTLQLTATHGRLDFDAPWLSFEASA
                     VFRSPFFFETARGRLDWMQTGDGGWRIDIGRLELATPGIAAIVTGSVTLPAGGGAPYL
                     DLSAEVPRLQVGAAPTYLPDQVIGEGGMDWLDRGLVAGSGYDGHVRLRGRADGFPYRD
                     GDGEFEARFRVEDTTLDFAPGWPRLENLEAGVVFAGPGLTVQAESATLLGVPVTAEVR
                     IADLEHTLVEVDGVSRADGAQLQRILRETPVRARLGPFAEAMTLGGPHTVDLALRIPT
                     DGRPMDGRGRIHFLGGTLDLPDAGWHFTHLHGALDFDDGSLSAENLGLLFGDEPMRLD
                     VASTGGAGNGETVFRLAGRYRLQTLLGEAADELSGSGRDALASGETDGVLTLTVPAAR
                     AGAPQYVLGFDSPLSGLALDLPAPLGKRAEDKRPLALKVEVGTRERARLSLDYGRELR
                     VLLELDDFPQAPRIERGDIRYNAGAPRLAQGSGVRASVVLDRWSPAGDDAPAPAARGR
                     NRAAAQPAAPALKLPQLPPWLKRIDLQIGELELGEQRIQRLKAGIVPGEDGLRVDLDA
                     PALAGRVLLPANPGAQAPLRFNLQRLYLHRTDDDDDDDRAANPAGAGPQTDPRGLPPM
                     RIAVDDLRLNDRPLGRLTLNAEAVRNGLRIADGGLGLHGAGHDLDGSGEWLMTPTGAS
                     THLLLTLKSAAIGETLTAFGFDPGIQGGKGTVKADLYWPRPVDDLELAVLSGVLHAEI
                     EEGQLLEVEPGVGRVLGLVSLTAIARRLTLDFSDIFGTGFAFDEIRAQLRFDDGISHT
                     EKFHMSGPSARVEISGTADLRQRTVDQHVIVTPRMGAALPIAGAIAGGPVGAAAAFVL
                     DRVVGRGIDRIGRREYDIRGPWKAPKVEPSSSAPTTPEKGPAVPVQPGGKPAR"
     gene            complement(6342..>6958)
                     /locus_tag="PK144_12965"
     CDS             complement(6342..>6958)
                     /locus_tag="PK144_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNF66682.1"
                     /translation="KSGGYLIIDQTEAMTTIDVNTGGYVGHRNLEETIFKTNLEAALA
                     IARQLRLRNLGGIIIIDFIDMQSTEHRLAVFKALEKALERDHAKSNVCEVSALGLVEM
                     TRKRTRESLEHVLCEPCPLCSGRGTVKTAETVCYEIFREILRNARQYEAAREFLVLAA
                     TEVVDLMLDEESASVAELEAFIGTPLRFQAEVLYPREQYDVVLL"
BASE COUNT          997 a         2516 c         2419 g          926 t
ORIGIN      
        1 gcggtcgagc cggcgcaggt cgccctgcag gcggctggcg aggtacaggc cgaggatgtg
       61 cagcgtcagc gcgtgaccac cgaagctctg gcaggcgcag tgatatgtgg tgtcctgcgc
      121 ggcttcatcc tcgctcagcg tctgcccgcc gatcctgtgc acaccgagcc ggtgcagcag
      181 ccgcgcgccg tcggcggcgc acaggttgtc gagccggtgg cgacgcaccg cgccatcggc
      241 gccgtagtgc tgcagatcgg cgatttcggc gcgggtgctc agcacgcaca gcgactgccg
      301 gccggcatcg gccagcgtcg tcagcagcgc cttcaggcca tcggtgcgca gccggtacga
      361 accggcgtcc tgcaacggct cgacgccatc aagcaccagc agcgcccgct gccggtccag
      421 cgcctgcgcc agccgccgcc cgcgatccca ggcgctggcc tgcgcctcgc agcgcacctc
      481 gaagaacgcc agcgccgcgt tcaggaacgg ctcgtcggtc gccgtccccg ccccggcatc
      541 gttgacgccc tgccgataga agctgtacgc aaacacccgc tgcgccccgc cccagccgtt
      601 caccttcagc cgctgcagcc agcgccgcag cagcgaggtc ttgccggtgc cacccggcgc
      661 caccagcacc gccaccgaca ccgcaccggc cgtctgccac tgcgcatcga gccattcgag
      721 gtcggcaccg cggccgaaga aatcggcggc gccttcgggg aggtggtgga gatcgacgtg
      781 cggcgcctct cgctgcctgg ctgcagcggc ggtcgccaac cgcccggcga tttctccggc
      841 cagcgcggcc agctcggctt cggcctttgg cttgccgagg cggctcaggg ccttgtcgcc
      901 tttcgggcgc atgttgatcc gggccagcca gtcaaccttc gaccatgcac acggccgcac
      961 gagtaccgga atgaccgtca gcccttccgc agcccgacgc tgcagcagcg ccgggatttc
     1021 ggtgcgtttg atgaaaggcg aactcagata cgaagcagaa accagcaaca gcgcgaactg
     1081 acattccgcc attgcccgct cgatcgccgg caaccaatcc tcaccgggtc cgatgcgggt
     1141 gtcgtcccag ccctccagtt cgatggcacc gagatgctcg gtcgcaagtg ccagccagtc
     1201 ggcatcctca cggctgtaac tgatgaacac cttgcccggc atcgtccgcc ctcccctgtt
     1261 ccctgctgcg ccgccgagcg tagcggccgg ggctgcgacg aaacaacacg cgggggctgt
     1321 ggggcgggct tcggctgcgt gcagccggtc ggccatccgg agacaggtct gctgcgccgc
     1381 aggcaaatcc atgccggagg tgttggaaaa ttttaccggc acttgttaca gccgcggacc
     1441 ctgccggcaa acccttgccc ggcaagggga aattttcgca tccagaaatg gaacgaggat
     1501 tgcttatcag cgaccggaat tgaccgcaac gacagtccgg gaatcgaccc ggtcgcttga
     1561 gaggctccct gatgaaatcc ctttccctca ccggcctgct gctggccacg ctcgcaggca
     1621 gcacggccgt cgcgctgccg ctggtcgacc agaccggcaa cctgctcagc aacggcaact
     1681 tcgagtccgg cagcacgacc ccgaccaatt ccggcagcgg cgtgagcacc ttctcgtcgc
     1741 tgacgggctg gaaccagtac tccaacaccg ggccggtgac gagcacatgg gcgggtacgc
     1801 cggtggtcga gggcgatcac agcgcgcgca tcaccggcaa caccaacgac ggcctgtacc
     1861 agtacaacgg cggctacacc ggcacctaca cgctgagcgc ctgggtctat gcgctgagcg
     1921 gcagcgcgca cctggtgctc gcgtggaaca gcggcagcac cacggccatc ggcagcagct
     1981 cgacgctgac cggccagtgg gaatacctga gcgtcaccgc cacgatgaac ggcagcctcg
     2041 gcggcccggt gctctacggc gccagcaaca acgccgattt ctacatcgac ggcgtctgga
     2101 tcaacgccgg cagcaccagc agctcgccga tggaccccgg caccggcttc aacccgaacg
     2161 ccatcagcac cgacgtgccg gaaccggcca gcgccgcgct gctgctcggc ggcctcggcc
     2221 tgctcgcccg ccgtcgcacg cgccgctgat cggccgacac cgcgggtgat ccgcnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcagc gtgccggctt tccgcccggc
     2401 tgcaccggca ccgccggtcc cttctccggc gtcgtcggcg ccgaggacga cggttcgacc
     2461 ttcggcgcct tccacggacc gcggatgtcg tactcgcgcc ggccgatgcg gtcgatgccg
     2521 cgtccgacca cgcgatcgag cacgaaggcc gccgccgcgc cgaccgggcc gccggcgatg
     2581 gcgccggcga tcggcagtgc cgcgcccatg cgcggcgtca cgatcacgtg ctgatcgacc
     2641 gtgcgctggc gcaggtcggc cgtgccgctg atctcgaccc tcgccgacgg cccgctcatg
     2701 tggaatttct cggtgtggct gatgccgtcg tcgaagcgca gctgcgccct gatctcgtcg
     2761 aacgcgaagc ccgtgccgaa gatgtcgctg aaatccagcg tcagccggcg cgcgatcgcg
     2821 gtcagcgaca ccagcccgag cacgcgcccg acgccgggct cgacctccag caactggcct
     2881 tcctcgatct cggcgtgcag cacgccggac agcaccgcca gttcgaggtc atcgaccggg
     2941 cgcggccagt acaggtcggc cttcaccgtg cccttgccgc cctggatgcc gggatcgaag
     3001 ccgaaggccg tgagcgtctc gccgatcgcc gcgcttttga gcgtcagcaa cagatgcgtg
     3061 ctggcgccgg tcggcgtcat cagccattcg ccgctgccgt cgaggtcgtg accggcgccg
     3121 tgcaggccga ggccgccgtc ggcgatgcgc aggccgttgc gcacggcctc ggcgttcagc
     3181 gtcagccgcc cgagcggacg atcgttcagg cgcaggtcat cgacggcgat gcgcatgggc
     3241 ggcaggccgc gcggatcggt ctgcgggccg gcgccggcgg ggttcgcggc gcggtcatcg
     3301 tcgtcatcat cgtcggtgcg gtgcaggtac aggcgctgca ggttgaagcg cagcggcgcc
     3361 tgcgcgccgg ggttcgccgg cagcagcacg cgtccggcga gcgccggtgc atcgagatcg
     3421 acgcgcaggc cgtcctcgcc gggcacgatg ccggccttga gccgctggat gcgctgctcg
     3481 ccgagttcga gttcgccgat ctgcaggtcg atgcgcttca gccacggcgg cagctgcggc
     3541 agcttcagcg ccggggctgc cggctgcgcc gctgcgcggt tgcgcccgcg cgcggccggt
     3601 gccggcgcgt cgtccccggc cggtgaccag cgatcgagca cgacgctggc gcgcacgccg
     3661 ctgccctgcg ccagccgcgg cgcgccggcg ttgtagcgga tgtcgccgcg ctcgatgcgc
     3721 ggggcctgcg ggaagtcatc gagttcgagc agcacgcgca gctcgcggcc gtagtcgagg
     3781 ctcagccggg cacgctcgcg cgtgccgacc tcgaccttca gcgccagcgg gcgcttgtcc
     3841 tcggcacgct tgccgagcgg cgccggcagg tcgagcgcga ggccgctcag cgggctgtcg
     3901 aagccgagca cgtactgcgg cgcgccggcg cgcgcggccg gcacggtcag cgtcagcacg
     3961 ccgtcggtct cgccgctcgc gagcgcatcg cggccgctgc cggacagctc gtcggcggcc
     4021 tcgccgagca gcgtctgcag ccggtagcgg ccggcgagcc ggaacacggt ctcgccgttg
     4081 ccggcgccgc cggtgctggc gacatcgagg cgcatcggct cgtcgccgaa cagcaggccg
     4141 aggttctcgg cgctcaggct gccgtcgtcg aaatcgagcg cgccgtgcag gtgcgtgaaa
     4201 tgccagccgg catcgggcag atcgagcgtg ccgccgagga agtggatgcg gccgcgcccg
     4261 tccatcggcc ggccatcggt cgggatgcgc agcgccagat cgacggtgtg cggcccgccg
     4321 agcgtcatcg cctcggcgaa gggcccgagg cgcgcgcgca ccggcgtctc gcgcaggatg
     4381 cgctgcagtt gcgcgccatc ggcacggctg acgccgtcga cctcgaccag cgtgtgttcg
     4441 agatcggcga tgcgcacttc ggccgtgacc ggcacgccga gcagcgtcgc cgattcggcc
     4501 tgcaccgtca ggcccggacc ggcgaacacg accccggctt cgaggttttc gagccgcggc
     4561 cagccgggcg cgaagtcgag cgtcgtgtcc tcgacgcgga agcgcgcctc gaactcgccg
     4621 tcgccgtcgc ggtacgggaa gccgtcggcg cggccgcgca ggcgcacgtg gccgtcgtag
     4681 ccgctgccgg caaccagccc gcgatcgagc cagtccatgc cgccttcgcc gatgacctga
     4741 tccggcagat aggtcggcgc agccccgacc tgcaggcgcg gcacctcggc gctgaggtcc
     4801 agatacggcg caccgccgcc ggccggcagc gtcaccgagc cggtgacgat cgcggcgatg
     4861 ccgggggtcg cgagttcgag gcgtccgatg tcgatgcgcc agccgccatc gccggtctgc
     4921 atccagtcga ggcgcccgcg cgcggtctcg aagaagaacg gcgagcggaa caccgcgctc
     4981 gcctcgaagc tcagccacgg cgcgtcgaaa tcgagccgcc cgtgcgtcgc cgtgagctgc
     5041 agcgtgccgt cgagccggtc gatgccgggc acggcgccat cggcgtgcac gcgcaggtcc
     5101 tcgaccagcg ccgccgccgc ccaggcgtcg gggtgcgccg gatcgaagcg caggcgggcc
     5161 tgcgggatgc gcagcgaagg ctgcagccgc gcaagccagc cgccggcttc ggggccggcc
     5221 tgcgcggcgg cgaggcgcag cagcgcatcg accggcagat cggtcgcgcc cagttcgagc
     5281 tggccatcgg ccgcgcgctg cacgccgagc gtgccgcctt cgatgcgcgc accgtcggcg
     5341 tcgtgggcgc tgtaatcgag cgccgcctgc caggggccat cggggttcgt cgccacgtcg
     5401 tcgcggcgcc agtcgaagcg caggccgagc cgcggcagcc cggcaagcag cggcgcgagc
     5461 cccggcagcg ccggcagtgc ggccggctgc agccggtcgg catcgagccg cccgagcagg
     5521 cgcgtgaccg tgccgtcagc ccagtcgccc cagagctcgg catcgaccgt gccgctcgcc
     5581 gggcgggcgg cgaacggcag ccgggcgaga tcgagccggg ccgccttcag ccagaactcg
     5641 ccgcgccaga tcgccggcgt gtcggcggca ccgtgcaggg ccagcaccgc ctgcacctcg
     5701 ccgtcgccgg gcgtgcgcag cgccacgccg gcccggcgct cgccggcacc gccgtgcagc
     5761 gtcaggcgca ggtcgcgata gaccagcgtc tgcgccgggg cctgcggatc gacgatctgc
     5821 agctcgccgt cgcgcagatc gaagcggcgc acctgcgcga gccacgcgag cagggattcg
     5881 agcggcaccg gcgcgccacc ggactccgcc tgcaggcgca gggcgccgtc cgggccggtt
     5941 tcgagccgca cgcgcacgcc ggccagttcg agcgccgaaa ccaccggccg gcgctggcgc
     6001 agcgaggcca gcgggtcgag accgaccgcg gcgcgctcga aggcgagcag caccgcaccg
     6061 ccggccggat cgcgcacgcg cagctcccgg acttcgagcg ccggtcccca gccgtaccag
     6121 ccggcgtgca gctcgccgag cgtgaccggc gccccgagcg tctcgccgag ccaggtctcg
     6181 atttcggggc ggtggtcagc gagctgcggc aggaacagcc gggccagacc gacgagcagc
     6241 gcggccacga acagcagcgc cagcccgatc cggtgccccc agagccaggt gtggcgcgcg
     6301 accggatgca gcagccggcg catcgcgaag cctccgggcg gctacagcag cacgacgtcg
     6361 tactgctcgc gcggatacag gacctcggcc tggaagcgca gcggcgtgcc gatgaaggct
     6421 tccagctcgg cgacgctggc cgattcctcg tcgagcatca ggtcgacgac ctcggtcgcg
     6481 gccagcacga ggaattcgcg cgcggcctcg tactggcggg cattgcgcag gatctcgcgg
     6541 aagatctcgt agcagaccgt ctcggcggtc ttgaccgtgc cgcggcccga gcacagcggg
     6601 cagggctcgc agagcacgtg ttcgaggctc tcgcgcgtgc gcttgcgcgt catctcgacc
     6661 aggccgagcg ccgagacctc gcagacgttg ctcttggcgt ggtcgcgctc cagcgccttc
     6721 tccagcgcct tgaacaccgc gaggcggtgc tcggtcgact gcatgtcgat gaagtcgatg
     6781 atgatgatgc cgccgaggtt gcgcaggcgc agctggcggg cgatcgcgag cgcggcttcg
     6841 aggttggtct tgaagatggt ctcttcgagg ttgcggtgac cgacgtagcc gccggtgttg
     6901 acatcgatcg tcgtcatcgc ctcggtctgg tcgatgatca ggtagccgcc ggacttca
//
LOCUS       DAOQIE010000215         6945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5770_length_6945_cov_7.914659, whole genome shotgun sequence.
ACCESSION   DAOQIE010000215 DAOQIE010000000
VERSION     DAOQIE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_6945_cov_7.914659"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..471)
                     /locus_tag="PK144_12970"
     CDS             complement(97..471)
                     /locus_tag="PK144_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66683.1"
                     /translation="MPVPTFQVPHPAPGRGAQAAAQGMNAQTPAYLQQQKPVDPQLLA
                     AARAALAQLDAALHAARPEAVKALKDADRRHVVEALNAAAAEVTARLGALPAATRRTL
                     VRDSAAHSPRLAAWFAKHGYDD"
     gene            complement(716..1456)
                     /locus_tag="PK144_12975"
     CDS             complement(716..1456)
                     /locus_tag="PK144_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66684.1"
                     /translation="MDKNSLLKGLSAALLLTASGSAPAAVSYFNDFSGPSDGKWSNYT
                     TYTTTQATPNSVLGRFAGTGTGTGSTLSLTGLDSHSSVTVEFDLYLFDTWDRHYTGTQ
                     ASPGAVNPYWSPDIVNVTANGTQYASLSYEYASYPYSNTLTGATYTPVSSGQYGGNPQ
                     WNNLATDRIFHITLTFSDLAPNLNLSFATASNSGLSDESYGIDNVRVSTVVSAPSSML
                     LLGLGVVMAARRRYRANSGHSGMQAMAA"
     gene            complement(2285..3181)
                     /locus_tag="PK144_12980"
     CDS             complement(2285..3181)
                     /locus_tag="PK144_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF66685.1"
                     /translation="MPAAAVSPYLLLVLTTLFWSGNFVLGRAVHGEIPALALSFWRWA
                     IALLLVLPLAWPQLRAHRGLLLGHWRSLTLLGVLGITNFNSFVYLGLRDTTATNAVLM
                     VSTAPVLIVLLSFLLLGQRPTPRQALGVVVSLSGVAVIVTRGDWHVLAQLALNLGDAW
                     VFAAVVSWALYSVCLRWRPAGLPPLAFLAATMLTGLLALIPIYLWDRLVAGHVLAINS
                     VTLASIAYVAIFPSVLAYIFWNRAVAELGPNRCGQFLHLMPAFGALLSWLLLDERLYA
                     YHWAGIALIATGLWLATAGRRS"
     gene            complement(3287..4471)
                     /locus_tag="PK144_12985"
     CDS             complement(3287..4471)
                     /locus_tag="PK144_12985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNF66686.1"
                     /translation="MPLPALLRRVSGPLSCCWMLFVCCLGVLPARAADEPRLSARSAI
                     LLDAGTGEILYERDPHAALPPASTTKVLTALIALERLDPAARLPVSPLAALAAPSRVG
                     LHPGDRVSAEDALYGLLLKSGNDAAEVLAEAVGGSVPGFAQLMNARARQLGALNSHFE
                     NPHGLPNDEHVSSAYDLAVIFRYAMLNPAFTKIVQTPNIWLHVERAAARGQAVPVMVA
                     THNRLLGSYEGAAGGKTGFTNKARRCYVGEVERGDKRLIVAVLGSNALWEDVRNLFDY
                     GFARYGLATPPRATPAVLITQARPNRGQRVTMIVPPRSPAKSAVRPGVAGKPPARPAA
                     RIAAARSPAAKPAARVTATSAPAAKPAARVAAAGKAPAAKKPAAAPAAKTVTASRRVA
                     RN"
     gene            complement(4566..5396)
                     /locus_tag="PK144_12990"
     CDS             complement(4566..5396)
                     /locus_tag="PK144_12990"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF66687.1"
                     /translation="MARILIAGCGDVGTRLGLQLAADGHAVWGLRRDPASLPAPIRPL
                     AADLASGSGLDALPDGLDHVVCTIAAGGGGEAAYRQAYVEAPLRLFDTLERRGARIER
                     VLFTSSTSVYGQDDGSEVDETAPAEPASPTAALLRRGEQVFLDGPYPAIVLRLAGLYG
                     PGRTWLIDQLRTGRARCTPGVWSNRIHVLDAARACAHLLFLREPQPLYNGADDHPAPL
                     CEVLDWLAGRLGLPAPARGTAADAMLRGNKRVSNRRLRRSGFQLRHPGYRDGYVDMLE
                     "
     gene            5439..6530
                     /gene="mutY"
                     /locus_tag="PK144_12995"
     CDS             5439..6530
                     /gene="mutY"
                     /locus_tag="PK144_12995"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNF66688.1"
                     /translation="MTDRSPVVEAFAARVLDWHARHGRHDLPWQREPTAYRVWISEIM
                     LQQTRVATVIPYYERFLQRFPDVAALAAAPLDEVLHLWSGLGYYARARNLHRAAQQLM
                     ARHAGAFPQTPAEVAGLPGIGRSTAAAILSLAHGQPLAILDGNVRRVLARHAGIEGWP
                     GAPAVLARLWTLAEALAPRERTGTYNQAMMDLGATLCTRTRAACTRCPVAADCSARRS
                     GRQRELPAPRPVRSLPVRRTRMLLIEDDGARVLLERRPPAGIWGGLWSLPECPVEADV
                     PDWCCTRLGLRVEQRGRLAGFRHTFTHFHLDIEAERLQLAGCAGRLMDDGGWLWYNPR
                     LPDALGLATPVSRLLASLRSDPDPEEIRR"
     gene            6527..6799
                     /locus_tag="PK144_13000"
     CDS             6527..6799
                     /locus_tag="PK144_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNF66689.1"
                     /translation="MSRMVHCVKLGQEAEGLDRQPYPGALGKRIFEQVSKTAWQQWLR
                     HQTMLINEYRLTPIEPKARKFLEEEMEKYFFGTGSTAPQGYVPPKS"
BASE COUNT         1096 a         2254 c         2552 g         1043 t
ORIGIN      
        1 atctggctgt atccgctggc atacgtgctc ggcatcggcg cgatcgtggc gttctaccgc
       61 tgagccggat gcggaggcgg cggtgccgcc ggtcggctag tcgtcgtagc cgtgcttcgc
      121 gaaccaggcc gcaagccgcg ggctgtgtgc ggcgctgtcg cggaccagcg tcctgcgcgt
      181 ggcggccgga agcgcgccga ggcgggcggt gacttcggcg gcggcggcgt tcagggcttc
      241 gacgacatgg cggcgatccg cgtccttcag ggccttcacg gcttccggac gtgcggcgtg
      301 cagggcggca tcgagttgcg cgagcgcggc gcgggcggcg gcgagcagtt gcggatcaac
      361 cggtttctgc tgctgcagat aggccggtgt ctgcgcgttc atgccttgcg cggcggcctg
      421 tgccccgcgg ccgggggcgg gatggggcac ctggaaggtc gggaccggca tcgtggcgat
      481 gacctccgca ttggcatgac cacgtcccgg atcatccggc ggcgggaggc ccgggacggt
      541 ggggtgaccg gccgaaaccc ggaaacggcc gagttatagc attcaggcag gatgcaggaa
      601 tgatcaatcc gggcaggggg cgggcgaagg tgctgacaga tgcgccgcca cccggaaagg
      661 tacgggtggc atcaccgatc ctgcagtttt gccctgccgc gtgcagtgcc gggtctcagg
      721 cagccattgc ctgcatgccg gaatgaccgg agttcgcccg ataacggcga cgggcggcca
      781 tgacgacacc gagaccgagc agcagcatcg acgaaggggc agaaacgacc gtggaaacgc
      841 ggacgttgtc gataccgtag gattcgtcgg acaagccgct gttactcgcc gttgcgaagc
      901 tcagattgag attcggtgcg agatcggaaa aggtcagcgt gatgtggaag atcctgtcgg
      961 tggcaagatt gttccattga gggttgccac catactggcc ggagctgacc ggagtgtacg
     1021 tggctcctgt cagggtgttg ctgtacgggt agctggcgta ttcgtaactg agggaagcgt
     1081 actgggtgcc attggccgtg acgttgacaa tgtccggcga ccagtaagga ttcaccgcgc
     1141 ccgggctggc ctgggtgccg gtgtagtgtc gatcccaggt gtcgaacaga tagagatcaa
     1201 attcgaccgt cacggaggaa tggctgtcga gaccggtcag cgacagcgtg gagccagtac
     1261 cggtgcccgt gccggcaaat cgtccaagaa cggaattcgg ggtggcctgg gtcgtcgtgt
     1321 atgtcgtgta attcgaccac ttgccgtcgc tgggacccga gaagtcattg aaataggaaa
     1381 cggcggcagg agccgatccg gaagctgtga gcaggagtgc ggccgaaagg cccttgagca
     1441 gtgaattctt gtccatgttt cccttccgat gatgctttcg gcagcgcgct accccctgcc
     1501 gtaccagggc ggtacctgaa tctcgcacgc tgctccaggc agcgtgcccg ctcggaccat
     1561 ccccaccagg cgctgctgtt cggcagtggg gcagaaggct tgcaggatcg tggctgcgtc
     1621 ggttggctcc gacgggggcg ctcgatccgc agcgcgggca aagcatccag tttttgactg
     1681 gcgcgaggtt agcgtctggg gtcggtcacg taaaggatgg caacagccgg aacgcacggg
     1741 ccgcaggcct catgccggcc gggcatcccg tctgcgccgc ctgccgcgcg ctcggtggcc
     1801 gggggcaaag cggcatggat catggcgacc ggggctgcgg gtttccggat ttccgtcgtt
     1861 gcagccctga attcggctgc gcagcactgc atttcgaaat ggttcctgaa taacgaaata
     1921 ttcatgaacc ggatgaaaga tgatacgtcc gctgcgcggc gggtccccgg ctcgccggca
     1981 tccggatgcg tccggcaaaa cgtgtctgat gaatcaggca cgtatcgcga ccggtggatg
     2041 attcgaacgg cggctgtcag ggacttgcgc ccatgcctgg gagcggtcgc cggtgatcgg
     2101 gccagggcgc cggatgcagg ccggtctcgc tggatgtgtg caggtgaggc agcacgggcc
     2161 ggatcgtcct gtgtggcccg gccgcgccgg aggcatcacg gttgtggcgc cctggtctgg
     2221 ttcagtgaat gtgaaccatt gtgccgatgg gggttcgttc gcttcaggca gcgcggttcc
     2281 ggtttcagga ccgccggccg gcggtcgcca gccacaggcc ggtggcgatc agtgcgatgc
     2341 ccgcccagtg gtaggcgtac aggcgctcat cgagcagcag ccaggacagc agcgcgccga
     2401 aggccggcat caggtgcagg aactggccgc agcggttcgg gccgagttcg gcgacggcgc
     2461 ggttccagaa gatgtaggcg agcaccgacg ggaagatcgc gacgtaggcg atgctcgcga
     2521 gcgtcacgga attgattgcc agtacatgcc cggcaaccag ccggtcccac aggtagatcg
     2581 ggatcagcgc cagcagtccg gtcagcatcg tcgcggccag gaacgcgagc ggcggcaggc
     2641 cggccggccg ccagcgcagg cagaccgaat acagcgccca gctgaccacg gccgcgaaca
     2701 cccaggcatc gcccaagttc agcgccagtt gcgcgagcac gtgccagtcg ccgcgggtga
     2761 cgatgacggc aacccccgac agcgacacga ccacgccgag cgcctggcgc ggcgtcgggc
     2821 gctggccgag cagcaggaag gacagcagca cgatcagcac cggcgccgtc gacaccatca
     2881 gcacggcatt ggtggccgtg gtgtcgcgca ggccgaggta gacgaagctg ttgaagttgg
     2941 tgatgccgag cacgccgagc agcgtcagcg aacgccagtg cccgagcagc aggccgcggt
     3001 gggcacgcag ttgcggccag gcgagcggca gcaccagcag cagcgcaatg gcccagcgcc
     3061 agaacgacag cgccagcgcc ggaatctcgc cgtgcacggc gcgcccgagc acgaagttgc
     3121 cggaccagaa cagcgtggtc agcaccagca gcagatacgg ggacacggcg gcggcgggca
     3181 tggccgggct ccggcggcag gttcacggac tcggggacaa catcgcccgc cgggcgtggc
     3241 cgccgggcgg gcgatggaag ggcggccgcg atgcggccgg ctcgcttcag ttgcgggcga
     3301 cgcggcggct ggcggtcacg gtcttggccg ccggtgccgc cgccggcttc ttcgctgccg
     3361 gcgccttgcc ggcggcagcg acccgggctg caggctttgc ggccggggcg gaagtggcgg
     3421 tgacccgggc tgcaggtttc gcggccgggc tgcgggcagc ggcgatgcgg gcggcgggcc
     3481 ttgccggcgg cttgccggcc acgcccgggc ggacggcgga cttcgccggg ctgcgcggcg
     3541 ggacgatcat cgtcacgcgc tggccgcggt tcggccgggc ctgcgtgatc agcacggccg
     3601 gggtcgcgcg cggtggcgtc gccagtccgt agcgggcgaa gccgtaatcg aacaggttgc
     3661 gcacgtcttc ccacagcgcg ttcgagccga gcacggcgac gatcaaacgc ttgtcgccgc
     3721 gttcgacctc gccgacgtag cagcggcggg ccttgttcgt gaagccggtc ttgccgccgg
     3781 cggcgccttc gtagctgccg agcaggcggt tgtgcgtggc gaccatcacc ggcacggcct
     3841 gaccgcgcgc ggccgcgcgc tcgacgtgca gccagatgtt cggggtctgc acgatcttcg
     3901 tgaatgccgg gttcagcatc gcatagcgga agatcacggc gaggtcgtag gcgctgctga
     3961 cgtgctcgtc gttcggcagg ccgtgggggt tttcgaagtg gctgttcagc gcgccgagct
     4021 ggcgggcacg ggcgttcatc agctgcgcga agcccggcac cgaaccgccg accgcctcgg
     4081 cgagcacctc ggcggcatcg ttgccggatt tcagcagcag gccgtacagc gcgtcctcgg
     4141 cgctgacgcg gtcgccgggg tgcaggccga cgcggctcgg ggcggcgagg gccgccagcg
     4201 gcgagaccgg cagcctggcg gccgggtcca gacgttcgag cgcgatcagc gcggtcagca
     4261 ccttggtcgt gctcgccggc ggcagcgcgg cgtgcggatc gcgttcatag aggatctcgc
     4321 cggtgccggc atcgagcagg atggcggaac gggcgctcag ccgcggctca tcggccgccc
     4381 gggccggcag cacgccgagg cagcagacga acagcatcca gcagcaggac agagggccgg
     4441 atacgcggcg cagcagggcc ggaaggggca tggagggctt acctcgggga gtgaacggcg
     4501 acggatcgcc gggcgcgcgg gctggccgat ccatgcggtc aaacggaaaa cgacgatgaa
     4561 gacggttatt caagcatatc gacatagccg tcgcgatagc cgggatggcg cagctggaag
     4621 ccgctgcggc gcaagcggcg gttcgatacg cgcttgttgc cgcgcagcat cgcatcggcg
     4681 gccgtgcccc gcgccggtgc cggcagcccg agacgccccg ccagccagtc gagcacctcg
     4741 cacagcgggg cagggtggtc gtcggcgccg ttgtacagag gctgcggttc gcgcaggaac
     4801 agcaggtgcg cgcaggcgcg cgcggcatcg aggacgtgga tgcggttgct ccagaccccg
     4861 ggcgtgcagc gggcgcggcc ggtgcgcagc tgatcgatca gccaggtgcg gcccggcccg
     4921 tacagcccgg ccagccgcag cacgatggcc ggatacggtc cgtcgaggaa cacctgctcg
     4981 ccacggcgca gcagtgccgc cgtcgggctg gccggttcgg ccggcgcggt ttcgtcgacc
     5041 tccgaaccat cgtcctggcc gtagacggaa gtgctcgaag tgaacagcac gcgctcgatg
     5101 cgcgcgccgc gccgctccag cgtatcgaac agccgaagcg gggcctcgac gtaggcctgc
     5161 cggtaggcgg cctcgccgcc gccgccggcc gcgatcgtgc agacgacgtg atcgagcccg
     5221 tccggcagcg cgtcgagacc gctgccgctg gccagatcgg ccgccagcgg ccggatcggt
     5281 gccggcaggc ttgccggatc gcggcgcagc ccccacaccg catgtccgtc ggcggccagc
     5341 tgcagtccga gccgggtgcc gacatcgccg cagccggcga tcagaatccg cgccatgacc
     5401 atatcctccg cccatcccgt tgccgcattc tcctgcccat gaccgatcga tctcccgtcg
     5461 tcgaagcctt tgccgcccgc gtgctcgact ggcacgcccg gcacggtcgt cacgacctgc
     5521 cgtggcagcg cgagcccacg gcctaccgcg tgtggatctc ggagatcatg ctccagcaga
     5581 cccgggtcgc caccgtcatc ccgtattacg agcgcttcct gcagcgcttc ccggacgtgg
     5641 ccgcgctcgc ggccgcgccg ctcgatgagg tgctgcatct ctggtccggc ctcggctact
     5701 acgcccgcgc ccgcaacctg caccgggccg cgcagcagct gatggccagg catgccggcg
     5761 cattcccgca gacgccggcc gaggtcgccg gcctgcccgg catcggccgt tccaccgctg
     5821 ccgcgatcct gtcgctcgcg cacggccagc cgctcgcgat cctcgatggc aacgtcaggc
     5881 gtgtgctcgc ccggcatgcc ggcatcgagg gctggccggg cgcaccggcc gtgctggccc
     5941 ggctgtggac gctggccgag gcgctggcgc cgcgcgagcg caccggcacc tacaaccagg
     6001 cgatgatgga tctcggcgcc acgctctgca cgcgcacgcg agcggcctgc acgcgctgcc
     6061 cggtggctgc ggactgcagc gcccggcgca gcggacgcca gcgtgaactg cccgcaccac
     6121 gaccggtccg cagcctgccg gtgcggcgca cgcgcatgct gctgatcgag gatgacggcg
     6181 cgcgcgtgct gctcgaacgc cgtccgccgg ccggcatctg gggcggactc tggtcgttgc
     6241 cggaatgtcc ggtcgaggcg gatgtgcctg actggtgctg cacgcggctg gggctgcggg
     6301 tcgagcaacg tgggcggctc gccggtttcc ggcatacgtt cacgcacttt cacctcgata
     6361 tcgaggccga gcgcctgcaa ctcgccggct gtgccgggcg gctcatggat gacggggggt
     6421 ggctctggta taacccgcgc ctccccgacg ccctggggct ggcgaccccg gtcagccgcc
     6481 tgctcgcgtc gctacgttcc gaccccgatc cggaggaaat ccgacgatga gccgcatggt
     6541 gcattgcgtg aagctcggtc aggaggccga agggctcgac cgccagccct atcccggcgc
     6601 cctgggcaag cgcatcttcg agcaggtttc caagacggca tggcagcagt ggctgcgcca
     6661 ccagaccatg ctgatcaacg agtaccggct gacgccgatc gagccgaagg cgcgcaagtt
     6721 cctggaggag gagatggaga agtacttctt cggcaccggt tcgaccgcgc cgcaaggcta
     6781 cgtgccgccg aagtcctgag caaagaaact ccggcacggg gcttgacgca cagcggtcat
     6841 gcggttttaa tgcgcgcctc gacgcgatgc ctcaatcgcg aatgtggcta ggtagctcag
     6901 ttggtagagc aggtgactga aaatcaccgt gtcggcggtt cgatt
//
LOCUS       DAOQIE010000216         6917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5798_length_6917_cov_6.590936, whole genome shotgun sequence.
ACCESSION   DAOQIE010000216 DAOQIE010000000
VERSION     DAOQIE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_6917_cov_6.590936"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PK144_13005"
     CDS             <1..131
                     /locus_tag="PK144_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNF66690.1"
                     /translation="VVSIGTFLQQFYIYGVEFVDHGYKVGMRRKELESLDYDDDED"
     gene            138..431
                     /locus_tag="PK144_13010"
     CDS             138..431
                     /locus_tag="PK144_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNF66691.1"
                     /translation="MIESTTPRYDWGLQVTVTDHVYNDDGTFPDVEEDALIIAAGSIG
                     EIVRVGLHEESNMPVYLVDFGIRVVGLSEDEIVPYEGDGSAPVSETSPPGVPA"
     gene            428..640
                     /gene="nifT"
                     /locus_tag="PK144_13015"
     CDS             428..640
                     /gene="nifT"
                     /locus_tag="PK144_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNF66692.1"
                     /translation="MKVMIRRDSAGKLSVYVPKKDLEEPVIEQEKDDLWGGTVRLANG
                     WLLGLPEMAADTRLPITVEARRLEGD"
     gene            643..909
                     /locus_tag="PK144_13020"
     CDS             643..909
                     /locus_tag="PK144_13020"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HNF66693.1"
                     /translation="MIQPAELADIGQSLETLTGTVGDLARALRERYPHLSFSVCDASD
                     VQEDPIAQTGRFDLHLIDAREHCLRLTQQFEQATGVLLAARRLH"
     gene            1090..1938
                     /locus_tag="PK144_13025"
     CDS             1090..1938
                     /locus_tag="PK144_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNF66694.1"
                     /translation="MSDDMLIDTLRAELAAGRVVPFLGPGLLALCADGSAVPASPLEL
                     VGVMTRKVSVPHKLKNKLPGAAQYIENFKHRKTLKNLMDTAYAPAVTPSPLHAWLAAQ
                     PLPLIVTTWYDDVMGAALASRSDWGRIQGVSQAEHRDGWTHAFAADGSRVEAGAAEAW
                     TTLLYQPTGAVAPAGNYIVSDSDYVEVLTEIDIQTPIPERVQQLRSERSFLFLGCRFD
                     DQLARSWARQISKRSAGRHYAVLPEEPTKNELRFMEEYGIERIELPLAEFAARLTGAG
                     AVAEPA"
     gene            2132..2443
                     /locus_tag="PK144_13030"
     CDS             2132..2443
                     /locus_tag="PK144_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66695.1"
                     /translation="MNTNTNVNARTAKHLRLIAAAAVLYAGFGAVAHAGDADSVYGEE
                     WRAVLQDRPGSYGYADRHMQPHMPVADAKRSSDGQRTCALDSDSPLNPRGGRNSQTGT
                     C"
     gene            complement(2528..3133)
                     /locus_tag="PK144_13035"
     CDS             complement(2528..3133)
                     /locus_tag="PK144_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF66696.1"
                     /translation="MAAQAVPQGFGPSTAQRLPVPEREAMSEAQRAAAQAIIDGPRKA
                     LFGPFVPLLQTPQLMECIGKTGETLRFGGSLPDRVRELAICIVARETHNQFEWLMHAP
                     LALKAGVAAEVIDCLLQGRRPRDLAPDETCAVDFVTELMHRHGVSDATYAEATGLFGE
                     PGTVELTALTGYFVSVCWIMNVARTPGPQDSPAGRLSAFPA"
     gene            complement(3168..3842)
                     /locus_tag="PK144_13040"
     CDS             complement(3168..3842)
                     /locus_tag="PK144_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNF66697.1"
                     /translation="MSTPAADTGIRIVGLFRGRRGVLPEEGQVSAIFREPLNAPCRLG
                     PEGIDGDRPVDRRWHGGPERALLHYPGEHYEYWRRHYPQHAAGFVPGGFGENLTTSGL
                     TEAQVRIGQRFRIGTALVEVSQPRRPCWKLDHRHDLPGLSRAVEATRRSGWLYRVCEA
                     GDFGPQSPLIPEGSPDPQAPSVQAVWDAWIDGTDPATLARLAGWPTLAPVWRQAFAQR
                     LAHRRG"
     gene            complement(3839..5047)
                     /locus_tag="PK144_13045"
     CDS             complement(3839..5047)
                     /locus_tag="PK144_13045"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNF66698.1"
                     /translation="MSTAELQNPSPSAADERRLLLAGIVALALAMGVGRFLYTPVLPL
                     MLREHALDAAGGGWLASVNFVGYLAGALYAALGVPAARRILVVALALVATALTTLGMG
                     LTDSYALWALLRFIAGVATALAMVLGGALVLERLPAARRASGSGYLYGGIGAGMAMTT
                     LAVAALDRLGVGTAGVWVLSGIATALLLPLCRPLLRPAPAAAAMARTACEAKPVRLGW
                     LVAAYGLAGFGYSVSATFLPVIVRTLGLPALVAESCWLVAGLAAIPASLLWGVIARRR
                     GTEFALAAALATEAAGVALPLLLPNAFGALAGGALFGASFLAVVGLALAVAREAEPTR
                     ASRAMGLMTASFGLGQILAPALSSALIGSGAGFGLPLALAAGALVVALLMLTRLHPQP
                     GVATRLETAP"
     gene            5156..6043
                     /locus_tag="PK144_13050"
     CDS             5156..6043
                     /locus_tag="PK144_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNF66699.1"
                     /translation="MDLQALRIFKAVADEGSVTRAAGRLHYVQSNVTARLRGLEEALG
                     TELFHRTGRRLAITPAGRTLLAYAEQILGLAEAARQAVRADDVPQGTLVIGSMETTAA
                     ARLPAALSAFHRAHPQVELQLETGTTEALLADVLAYRLDCALVAGPQHHPELHAEPVF
                     VEELVLITEAAHPPVGGPRDLHRPQMLSFRSGCAYRGLLERWLIDGGVRPARIASFGT
                     FEAIIGCVAAGMGVSLMPASVVEPHRRLGTIRTHRIEAAYAQVATLLVWRRDQARHAA
                     REAFQATLRACAGSTEQAA"
     gene            6225..6719
                     /locus_tag="PK144_13055"
     CDS             6225..6719
                     /locus_tag="PK144_13055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNF66700.1"
                     /translation="MTAFDSLEFRRTLGRFATGVCIAATVDADDHPVGMTINSFGSLS
                     LEPPLILWSIGNGSSLRPAFEKHGYFSINVLRVDQVALSNTFAMSPGDRFAGLDWTAG
                     LGGVPLLNDCLARFQCAVERVLPGGDHQLILGRVVALETFDGEPLLFYAGGYRRIGDS
                     AGGW"
     gene            complement(6735..>6917)
                     /locus_tag="PK144_13060"
     CDS             complement(6735..>6917)
                     /locus_tag="PK144_13060"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNF66701.1"
                     /translation="RGTTWRALPEDLRRQLDDGLARTLMLEQPALIRRPLLVRADGAL
                     RLGWDENGLADWLAGA"
BASE COUNT         1044 a         2460 c         2460 g          953 t
ORIGIN      
        1 acgtcgtatc gatcggcacc ttcctgcagc agttctacat ctacggcgtc gagttcgtcg
       61 atcacggcta caaggtcggg atgcggcgca aggaactcga atcgctggat tacgacgacg
      121 acgaggactg aacggccatg atcgaatcca ccacgccgcg ttatgactgg ggcctgcagg
      181 tcaccgtcac cgaccacgtc tacaacgacg acggcacttt tccggatgtc gaggaagacg
      241 cgctgatcat cgccgccggc agcatcggcg agatcgtgcg cgtcggcctg cacgaggaat
      301 ccaacatgcc ggtgtatctg gtcgatttcg gcatccgcgt cgtcgggctt tccgaagacg
      361 agatcgtccc gtacgagggc gatggctccg cacccgtttc cgaaaccagt cctccgggag
      421 tccctgcatg aaagtcatga tccgccgcga ttcggccggc aagctgtcgg tgtacgtgcc
      481 caagaaggat ctggaagagc cggtcatcga acaggaaaag gacgacctgt ggggcggcac
      541 cgtgcgcctc gccaacggct ggctgctcgg cctgccggaa atggccgctg acacccgcct
      601 gcccatcacc gtcgaagcgc gccggctcga aggagactga ccatgatcca gcccgccgaa
      661 cttgccgaca tcggccagag cctcgaaacc ctgaccggca cggtcggcga tctggcccgt
      721 gcgctgcgcg aacgctatcc gcacctgagc ttctcggtgt gcgacgcgag cgacgtgcag
      781 gaagacccga tcgcgcagac cgggcgcttc gacctgcacc tgatcgatgc ccgcgagcac
      841 tgcctgcgcc tgacgcagca gttcgagcag gccaccggcg tgctgctggc cgcgcgtcgg
      901 ctgcactgaa accgcactga tcccgcgatg ccgtcgcgcc ggttccgtca cccgcgggga
      961 cggcaccggc ggccggtgac ggaccgcgac cgtcgccggc gccggcgcgc cggccggcag
     1021 cagagcttcg cgaacgggcc ccagggcctg ctcgcaaccg aacgcaaccg cctccaggag
     1081 tccgccgcga tgagcgacga catgctgatc gataccctgc gcgccgaact ggccgccggc
     1141 cgcgtcgtac ccttcctcgg ccccggcctg ctggcgctct gcgccgatgg cagcgccgtg
     1201 ccggcctcgc cgctggaact ggtcggcgtc atgaccagga aggtttcggt gccgcacaag
     1261 ctcaagaaca agctgccggg tgccgcgcag tacatcgaga acttcaagca ccgcaagacg
     1321 ctgaagaacc tgatggatac ggcctacgcg ccggcggtga cgccgtcgcc gctgcatgcg
     1381 tggctcgccg cgcagccgct gccgctgatc gtcacgacct ggtacgacga cgtgatgggc
     1441 gccgcgctcg cgtcgcgcag cgactggggc cgcatccagg gcgtgtcgca ggccgagcac
     1501 cgtgacggct ggacgcatgc cttcgccgcc gacggcagcc gtgtcgaggc gggcgctgcc
     1561 gaagcctgga cgacgctgct gtaccagccg accggcgccg ttgctccggc cggcaactac
     1621 atcgtctcgg attcggatta cgtcgaggtg ctgaccgaga tcgacatcca gacgccgatc
     1681 ccggagcgcg tgcagcagct gcgcagcgag cgcagcttcc tgttcctcgg ctgccgcttc
     1741 gatgatcagc tcgcgcgcag ctgggcgcgc cagatcagca agcgctcggc cgggcgccac
     1801 tacgcggtgc tgcccgaaga gccgacgaag aacgagctgc gcttcatgga ggaatacggc
     1861 atcgagcgca tcgagctgcc gctcgccgaa ttcgcggcgc gtctgaccgg cgccggcgcc
     1921 gtcgccgaac cggcctgatc ctcgccatac cgaccgccgc agttccggaa gcagcgccgg
     1981 gactgcggcg gtcggctttt gtgccggctt cgtgcccggg ggagattttt gccggtcgct
     2041 gcatccgccg gccggctgcg cgcgaatagg gaagtgtgga gcttgttcgc tcccgtcccg
     2101 atcccgaaat ccttccccga ggagcgccgc catgaacacg aacacgaatg tgaatgccag
     2161 gaccgccaag cacctgcgcc tgatcgccgc tgccgccgtg ctgtacgccg gtttcggcgc
     2221 cgtcgcccac gccggcgatg ccgacagcgt ctacggcgag gaatggcgcg cggtgctgca
     2281 ggatcgcccc ggcagctacg gctacgccga ccggcacatg cagccgcaca tgccggtggc
     2341 cgacgcgaag cgcagcagcg atggccagcg cacctgcgcg ctggacagcg attcgccgct
     2401 caatccgcgc ggcggccgca acagccagac cggcacctgc tgaacacgct tcgccggctc
     2461 ttcgatccgg acggcccgcc tgctggcggg ccgttgcatt gcgggccggc ggggcgcagt
     2521 ccgtcgctca ggccggaaac gcggacagcc ggccggccgg ggaatcctgc gggccgggcg
     2581 tgcgggcgac gttcatgatc cagcacacgc tcacgaaata accggtgagt gccgtcagtt
     2641 cgacggtgcc cggttcgccg aacaagccgg tcgcttcggc gtaagtcgca tcgctgacgc
     2701 cgtgccggtg catcagttcg gtcacgaaat cgaccgcgca ggtttcatca ggcgccagat
     2761 cgcgcgggcg cctgccttgc agcagacagt cgatcacctc ggctgccact ccggccttga
     2821 gcgccagcgg cgcatgcatc agccattcga actggttgtg cgtttcccgg gccacgatgc
     2881 agatcgccag ttcgcgcacg cgatcgggca ggctgccgcc gaagcgcagc gtttctccgg
     2941 ttttgccgat gcattccatc agctgcgggg tctgcagcaa gggcacgaac gggccgaaca
     3001 gggctttgcg cgggccgtcg atgatggcct gagccgccgc gcgctgggct tcggacatgg
     3061 cttcgcgctc ggggaccggc aggcgttgcg ccgtcgaagg cccgaaaccc tgggggacgg
     3121 cctgcgcagc cactggttcg ttcatgccgg attctcccgg tgaaggttca gccgcgccga
     3181 tgcgccagcc gctgcgcgaa cgcctgccgc cagaccgggg cgagcgtcgg ccagccggcg
     3241 aggcgggcga gggtggcggg gtcggtgccg tcgatccacg catcccagac cgcctgcacg
     3301 ctcggggcct gcggatcggg gctgccctcg gggatcagcg gggactgcgg accgaaatcg
     3361 ccggcttcgc acacgcggta cagccagccg ctgcgccgcg tcgcctcgac cgcgcgggaa
     3421 agtcccggca ggtcgtggcg atggtcgagc ttccagcacg gccggcgcgg ctggctgact
     3481 tccacgagcg ccgtgccgat gcgaaagcgc tggccgatgc gtacctgcgc ctcggtcagg
     3541 ccgctcgtcg tcaggttctc gccgaagccg ccgggcacga agccggcggc gtgctgcggg
     3601 tagtggcggc gccagtattc gtagtgctcg ccggggtagt gcagcagcgc gcgctccgga
     3661 ccgccgtgcc agcggcggtc gaccggccgg tcgccgtcga tgccttcggg gccgagccgg
     3721 caaggagcat tcagcggctc gcggaagatc gcgctgacct ggccttcctc gggcagcacg
     3781 ccgcgccggc cgcggaacag gccgacgatg cggatgccgg tgtccgcggc cggcgtgctc
     3841 acggcgccgt ctccagccgc gtggcgacgc ccggctgcgg gtgcaggcgc gtcagcatca
     3901 gcagcgcgac gaccagcgcg ccggcggcga gcgcaagcgg caggccgaag ccggcaccgc
     3961 tgccgatcag cgccgaggac agcgccgggg cgaggatctg gccaagaccg aagctcgcgg
     4021 tcatcaggcc catggcgcgg ctggcgcggg tcggttcggc ctcgcgggcg acggcgagcg
     4081 ccagcccgac cacggcgagg aagctcgcgc cgaacagcgc accgccggcg agcgcaccga
     4141 aggcgttcgg cagcagcagc ggcagcgcga cgccggcggc ctcggtggcg agggccgcgg
     4201 ccagcgcgaa ttcggtgccg cgccggcgcg cgatcacgcc ccacagcagg ctcgccggga
     4261 tcgccgccag cccggcgacc agccagcagg attcggcaac gagtgccggc agcccgagcg
     4321 tgcgcacgat caccggcagg aaggtggcgc tgaccgagta gccgaagccg gcgagtccgt
     4381 aggcggccac cagccagccg aggcgcaccg gctttgcctc gcaggcggtg cgggccatcg
     4441 ccgcggcggc cggggccggg cgcagcagcg gccggcagag cggcagcagc agcgcggtcg
     4501 cgattccgct cagcacccag acgccggccg tgccgacgcc gagccggtcg agcgcagcga
     4561 ccgcgagcgt cgtcatcgcc atgccggcac cgatgccgcc gtacagatag ccggagccgg
     4621 acgcgcgccg cgccgccggc aggcgttcga gcaccagcgc gccgccgagc accatcgcca
     4681 gcgccgtcgc gacgccggcg atgaagcgca gcagcgccca cagcgcgtag ctgtcggtca
     4741 ggcccatgcc gagcgtggtc agcgcggtcg cgaccagcgc cagagccacc acgaggatgc
     4801 gccgggccgc cggcacgccg agggccgcgt acagcgcgcc ggcgaggtaa ccgacgaagt
     4861 tgacgctcgc gagccagccg ccgccggcgg catcgagcgc gtgctcgcgc agcatcagcg
     4921 gcagcaccgg cgtatacagg aagcgtccga cacccatcgc cagcgccagc gcgacgatgc
     4981 cggcgagcag cagccggcgt tcgtcggccg cgctcgggga gggattctgc agctcggcgg
     5041 tgctcatcgc ggcgccctcg cggacggtgg ggaatgtccg cgcagcctag tcgggatatt
     5101 ctatcgaata aaatgaatta ttctgttcag gtcctatcac cgggagcgat ggatcatgga
     5161 cctgcaggcg cttcgcatct tcaaggcggt cgcagacgag ggcagcgtca cgcgcgccgc
     5221 cgggcggctg cattacgtgc agtcgaacgt caccgcacgg ctgcgcgggc tggaggaggc
     5281 cctcggcacc gaactcttcc accgcaccgg ccggcggctg gcgatcacgc cggccgggcg
     5341 cacgctgctc gcctacgccg agcagatcct cgggcttgcc gaggctgcgc ggcaggccgt
     5401 gcgcgccgac gacgtgccgc agggcacgct ggtgatcggt tcgatggaga ccacggccgc
     5461 ggcgcggctg ccggcggcgc tgtcggcctt tcatcgcgcg catccgcagg tcgagctgca
     5521 gctcgaaacc ggcacgaccg aggcgctgct cgccgacgtg ctcgcctacc gcctcgactg
     5581 cgcgctggtc gccggtccgc agcatcatcc ggaactgcac gccgagccgg tgttcgtcga
     5641 ggaactggtg ctgatcaccg aggccgcgca cccgccggtc ggcgggccgc gcgacctgca
     5701 ccggccgcag atgctgtcgt tcaggagcgg ctgcgcctac cgcggcctgc tggagcgctg
     5761 gctgatcgat ggcggcgtgc gcccggcgcg catcgcaagc ttcggcacct tcgaggcgat
     5821 catcggctgt gtggccgccg gcatgggcgt gtcgctgatg ccggcgagcg tcgtcgagcc
     5881 gcaccggcgg ctcggcacga tccgcacgca ccgcatcgag gcagcgtatg cacaggtcgc
     5941 gacgctgttg gtctggcggc gcgatcaggc ccggcatgcg gcgcgcgagg ccttccaggc
     6001 gacgctgcgc gcctgcgcgg gcagcacgga gcaggcggca tgaacggcgg cggcggcaga
     6061 ctgcacccgc tgcacctcaa gcgggcagcg gccggccggc attgcccggt ggcggtgccg
     6121 ccggctgcgc cttacgatgc gcggcctcgt tccggccggg cggcccccgc gtccgggcct
     6181 gcccagtacg cccagtacgc ccagcttccg gggatcaccc gccgatgact gccttcgaca
     6241 gcctcgaatt tcgccgcacg ctcgggcgtt tcgcgaccgg cgtctgcatt gccgcgaccg
     6301 tcgatgccga cgatcacccg gtcggcatga cgatcaactc cttcggctcg ctgtcgctgg
     6361 aaccgccgct gatcctctgg agcatcggca acggcagttc gctgcgtccg gccttcgaga
     6421 agcacggcta cttcagcatc aacgtgctgc gcgtcgatca ggtggcgctg tcgaacacct
     6481 tcgcgatgtc gccgggggat cgcttcgccg ggctcgactg gacggcaggc ctgggcggcg
     6541 tgccgctgct caacgactgc ctggcgcgct tccagtgcgc ggtcgagcgc gtgctgccgg
     6601 ggggcgatca ccagctcatc ctcgggcgtg tggtggcgct cgaaaccttc gacggcgagc
     6661 cgctgctgtt ctacgccggc ggctaccggc gcatcggcga cagcgccggc ggctggtgat
     6721 tgcgggctgg ggactcaggc gccggccagc cagtcggcca gcccgttttc gtcccagccg
     6781 aggcgcagcg cgccatcggc acgcacgagc agcggccggc ggatcagcgc cggctgttcg
     6841 agcatcagcg tgcgggcgag gccgtcgtcg agctgacggc gcaggtcttc gggcagcgcg
     6901 cgccaggtcg tgccgcg
//
LOCUS       DAOQIE010000217         6855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5875_length_6855_cov_8.025588, whole genome shotgun sequence.
ACCESSION   DAOQIE010000217 DAOQIE010000000
VERSION     DAOQIE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5875_length_6855_cov_8.025588"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK144_13065"
     CDS             <1..606
                     /locus_tag="PK144_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66702.1"
                     /translation="RRPAPMHRLLILAVLVLVWGGWHWQATRPLRWPAGQIVDATPLQ
                     SLTTAPPFELRGFRLQPLAAFELDARALATERYRLDAGAALAPLDVAFGWGRMSDQSV
                     IDRLDIRQGARFYTWAYQDPPPLAPAGIVRSSANLHLIPADAGVERQLLAIRPGQRVR
                     LGGLLVEATAADGFRWRSSLSREDSGNGACELVWVEYVELH"
     gene            711..1703
                     /locus_tag="PK144_13070"
     CDS             711..1703
                     /locus_tag="PK144_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF66703.1"
                     /translation="MKIVVPLLVAAVALGGGYAGWRYWQAREAEALPAGIVAGNGRAE
                     ATELDVATKRAARVAEVLVDEGDTVTAGQVLARMDLREEAAALARARAELKRAQADRD
                     YAGAQLDLADSELTYSQQQLERSESLVKSGGVPREKLDADQHRQRSAVASRAAARIRI
                     IETEQAIEAAAAQVRQLEIALEDGELRAPRNGRVLYRLAEPGEVLGAGGKVLTLIDLD
                     DTWLTVFLPETVAGRLALGAEARVLLDAFPDRPLPARISFVSDRAQFTPKSVETREER
                     ERLSFRVKAQVQVPPQYRALAKPGLPGMAYVKLDAQAAWPPRLACTELSQPCRP"
     gene            1748..4501
                     /gene="rbbA"
                     /locus_tag="PK144_13075"
     CDS             1748..4501
                     /gene="rbbA"
                     /locus_tag="PK144_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNF66704.1"
                     /translation="MIEAVHHRYGRTPALDDCTLTLPAGGMIGLIGPDGVGKSTLLGL
                     IAGVKRLQQGAVTVLGGDVRSNAHRDALAQRIAYMPQGLGRNLYPTLSVAENVDFFAR
                     LFNLHAQARSRRRDALLAATGLAPFADRAAGKLSGGMKQKLGLCCALIHQPELLILDE
                     PTTGVDPLSRRQFWELVERLRAQSPDLSVLVATAYMEEAERFDTLVLMDAGRVLATGT
                     PAGLKAQAGADDLEATYIALLPEHRRLGHQALSIPPRPAGHGSAEAAIEAEGLTRRFG
                     RFTAVDHVSFRIGRGEIFGFLGSNGCGKTTTMKMLTGLLPASEGHARLFGTPVDATDP
                     ALRLRIGYMSQAFSLYRELTVQQNLVLHARVLRIAERDIAPRIDALIARFGLDGLRDT
                     VADALPLGVRQRLSLAVAVIHRPEILILDEPTSGVDPVARDDFWRLLGELARRDGVTI
                     FVSTHFMNEAARCDRISLMHAGRVLAQGTPAELVAASGTASLEAAFIRALEDAQAGPG
                     QASTKAPATATTDAAALAGLAAGGEELRAGHGFSLRRLLTFAWREALELRRDPIRLAF
                     ALLGTLLLMLVMGYGISLDVEDLPYAVLDLDRTPESRAYLDSFKGIRELAERAPATSS
                     GDIDARLLAGELSFALELPPGFGAGLRAGRRPEVGVRLDGAIPFRAETARGYLLGIHA
                     GFLADPRPAQLGPAPAETQTPLAGLEVRFRYNQDFRSVNAIVPGVLALLLLVIPATLT
                     ALGVVREKELGSITNLYASPATRLEFLLGKQMPYAGLGLVNAAVMALLTVWLFGVPLK
                     GSLPAFALGALLYVIAASAFGLLVSTVTSTQIAALFGTFILTMLPSINFSGLIAPVSS
                     MSGGAAVMGHSFPASYFLAIAVGTFTKALGFAALAPKYLQLTGFTLVYVLLAWRLLPK
                     QER"
     gene            4504..5637
                     /locus_tag="PK144_13080"
     CDS             4504..5637
                     /locus_tag="PK144_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66705.1"
                     /translation="MRSPPLRNVLELMRREFYALGRDRVMLGLIVYAFSVLIYVNATA
                     TGIELRNAAIAFVDEDASTLSARLFDAPQPPYFQHPQRLALADVDAALDAGRFTFVVD
                     VPPGFQGDVLAGRAPRLQINVDATAMSQAGIGANYLQQIFSAEIDEFVRGRRQEAPQA
                     VTQVVRIAFNPNLQMPWFMGPMMIVNSITLLSIVLTGAALIREREQGTLEHLLVLPLT
                     PIEIMLAKIGATALVIVVATTFATVVMIHGVLSVPARGSLALFLVAAFVYSASIAAIG
                     IFLATLARSMPQLGLLSIPVVFPMAMLSGGATPLDSMPVAVQRIMQFSPSTHFTAIVQ
                     AILYRGAGFDVIWPQFVAMLAIGAVFLAIAVHRLRTTLAQAGS"
     gene            complement(5756..6166)
                     /locus_tag="PK144_13085"
     CDS             complement(5756..6166)
                     /locus_tag="PK144_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66706.1"
                     /translation="MSLKATWYRDKLLKKFRRGFHGYPVATVAYYGPDDRLASKAVVG
                     IAPDENAEVEMLERWYAETGDIRQDPAISEAIVRYIESHAVRSVLTPGRIIGCPHEAG
                     VHYPEGGTCPVCTYWAGRDRFTGERLDGEKESDA"
     gene            complement(6322..>6855)
                     /locus_tag="PK144_13090"
     CDS             complement(6322..>6855)
                     /locus_tag="PK144_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNF66707.1"
                     /translation="ATAGLKVTSFRPSVIFGCEDSFFNRFAGLLKLAPVLPLACPEAR
                     FQPVYVRDVAAAFAQALEDERTVGKRLELGGPRIYTLKELVEFTARTLGLSTLVIGLP
                     DSVAKLQAKLMDKLPTRPFSLDNYRSLQQDSIVGGEDGFALLGIVPRSVEGVMPRELR
                     ARDHRGPYAAYRASAGR"
BASE COUNT          908 a         2484 c         2427 g         1036 t
ORIGIN      
        1 cgccggcctg ccccgatgca ccggctcctg atcctcgccg tgctggtgct cgtctggggc
       61 ggctggcact ggcaggccac gcggccgctg cgctggccgg ccgggcagat cgtcgacgcc
      121 acgcccctgc agagcctcac caccgcgcct ccattcgagc tgcgcggctt ccggctgcag
      181 ccgctcgccg ctttcgagct cgatgcccgt gcgctggcga ccgagcgcta ccgcctcgat
      241 gccggtgccg cgctcgcgcc gctcgacgtg gccttcggct ggggccggat gtcggatcag
      301 tcagtcatcg accggctcga catccgccag ggcgcacgtt tctacacctg ggcctatcag
      361 gacccgcccc cgctcgcgcc ggccgggatc gtgcgctcca gcgccaacct gcacctgatc
      421 ccggccgatg ccggcgtcga acgccagctg ctggcgatcc gccccggcca gcgcgtgcgg
      481 ctcggcggcc tgctggtcga ggccaccgct gcggacggct tccgctggcg cagttcgctg
      541 agtcgcgagg acagcggcaa cggcgcctgc gagctggtct gggtcgagta cgtcgaactg
      601 cactgacggc ggcgacgtcc gatgcccggc gcggctgacg ccgatcagcg cagccccgcc
      661 cgcggcggat agactccggc cgccttccag ccgatccggg agtcgatgcc atgaagatcg
      721 tcgtgccgct gctcgtcgcc gccgtcgcgc tcggcggcgg ctatgccggc tggcgctact
      781 ggcaggcgcg cgaggccgag gcgctgccgg ccggcatcgt cgccggcaac ggtcgcgccg
      841 aggccaccga gctggatgtt gcgaccaagc gcgcggcgcg cgtcgccgag gtgctggtcg
      901 atgaaggcga cacggtgacg gccggtcagg tgctggcacg catggacctg cgcgaggaag
      961 ccgccgcact tgcgcgggcg cgggcggagc tgaagcgcgc gcaggccgat cgcgactatg
     1021 ccggtgcgca gctcgatctg gccgacagcg agctgaccta ctcgcagcag cagctcgaac
     1081 gctccgagag cctcgtgaaa tccggcggcg tgccgcgcga gaagctcgac gccgaccagc
     1141 accgccagcg cagcgcggtc gcctcgcgcg ccgccgcgcg catccgcatc atcgagaccg
     1201 agcaggcgat cgaggccgcc gccgcccagg tccgccagct cgagatcgcg ctcgaggacg
     1261 gcgaactgcg cgcgccgcgc aacggccgcg tgctgtaccg gctggccgaa cccggcgagg
     1321 tgctcggggc cggcggcaag gtgctgacgc tgatcgacct cgacgacacc tggctgaccg
     1381 tgttcctgcc cgaaaccgtc gccgggcggc tcgcgctcgg ggccgaggcg cgcgtgctgc
     1441 tcgatgcctt cccggaccgg ccgctgccgg cgcgcatcag cttcgtatcc gaccgcgcgc
     1501 agttcacgcc gaagtcggtc gagacgcgcg aggagcgcga gcggctgtcg ttccgcgtca
     1561 aggcgcaggt gcaggtgccg ccgcagtacc gcgcgctcgc caagcccggc ctgccgggca
     1621 tggcctacgt gaagctcgat gcgcaggccg cgtggccgcc gcggctggcc tgcaccgagc
     1681 tctcgcaacc gtgccggccg tgagccgcgc tccggggcgg cccggggatg agcgcgcgcc
     1741 ggtcgcggtg atcgaggcgg tccaccaccg ctacggccgc acgccggcgc tcgatgactg
     1801 cacgctgacg ctgccggccg gcggcatgat cgggctgatc gggccggacg gggtcggcaa
     1861 gtcgacgctg ctcgggctga tcgccggcgt caaacgcctg cagcagggcg cggtgacggt
     1921 actcggcggt gacgtgcgct cgaacgcgca ccgcgacgcg ctcgcgcagc gcatcgccta
     1981 catgccgcag gggctcgggc gcaacctgta cccgacgctg tcggtggccg agaacgtcga
     2041 tttcttcgca cgcctgttca acctgcacgc gcaggcgcgc agccggcggc gcgatgcgct
     2101 gctcgcggcg accgggctcg cgccgttcgc ggatcgcgcc gccggcaagc tctcgggcgg
     2161 catgaagcag aagctcgggc tctgctgcgc gctgatccac cagcccgagc tgctgatcct
     2221 cgacgagccg accaccggcg tcgatccgct gtcgcgccgg cagttctggg agctggtcga
     2281 gcgcctgcgc gcgcagtcac cggacctgag cgtgctggtg gcgaccgcgt acatggagga
     2341 agccgagcgc ttcgatacgc tggtgctgat ggacgccggg cgcgtgctcg cgaccggcac
     2401 gccggcgggg ctgaaggcgc aggccggggc tgacgacctc gaagcgacct acatcgcgct
     2461 gctgcccgag catcgccggc tcggccatca ggcgctgtcg atcccgccgc ggccggccgg
     2521 gcacggaagc gcggaggccg caatcgaggc cgaggggctg acgcggcgct tcggccgctt
     2581 cacggcggtc gatcacgtga gcttccgcat cgggcgcggc gagatcttcg gcttcctcgg
     2641 ctcgaacggc tgcggcaaga ccacgacgat gaagatgctg accggcctgc tgccggcgag
     2701 cgagggccac gcccgcctgt tcggcacgcc ggtcgatgcc accgatccgg cgctgcgcct
     2761 gcgcatcggc tacatgtcgc aggccttctc gctgtaccgc gagctgaccg tgcagcagaa
     2821 cctcgtgctg cacgcgcgcg tgctcaggat cgccgagcgc gacattgcgc cgcgcatcga
     2881 cgcgctgatc gcgcgcttcg gcctcgacgg cctgcgcgac acggtggccg atgcactgcc
     2941 gctcggcgtg cgccagcgcc tgtcgctggc ggtggccgtg atccaccggc ccgagatcct
     3001 gatcctcgac gagccgacct cgggcgtcga tccggtcgca cgcgacgact tctggcgcct
     3061 gctcggcgaa ctcgcgcgcc gcgacggcgt gaccatcttc gtgtcgacgc acttcatgaa
     3121 cgaggcggcg cgctgcgacc gcatctcgct gatgcacgcc gggcgcgtgc tggcgcaggg
     3181 cacaccggcg gagctggtcg ccgcgagcgg cacggcctcg ctggaagcgg ccttcatccg
     3241 cgcgctcgaa gacgcgcagg ccggcccggg ccaggcttcg acgaaggcgc cggccacggc
     3301 cacgaccgac gccgccgcgc tggccggcct cgcggcgggc ggcgaggaac tgcgtgccgg
     3361 ccacggcttc tcgctgcgca ggctgctgac cttcgcgtgg cgcgaggcgc tggagctgcg
     3421 ccgtgacccg atccgcctcg ccttcgcgct gctcggcacg ctgctgctga tgctggtcat
     3481 gggctacggc atctcgctcg atgtcgagga tctgccctat gccgtgctcg atctcgaccg
     3541 cacgcccgag agccgcgcct acctcgacag cttcaaaggc atccgcgaac tggccgaacg
     3601 cgccccggcg acctcgtccg gcgacatcga tgcgcggctg ctcgccggcg agctgagctt
     3661 tgcgctggag cttccgcccg gcttcggcgc cgggctgcgc gccgggcgcc ggcccgaggt
     3721 cggcgtgcgc ctcgacggcg cgatcccgtt ccgcgccgag actgcgcgcg gctacctgct
     3781 cggcatccac gccggcttcc tcgccgatcc gcgtccggca cagctcgggc cggcaccggc
     3841 cgagacgcag acgccgctcg ccgggctcga agtgcgcttc cgctacaacc aggacttccg
     3901 ctcggtcaac gccatcgtgc ccggcgtgct ggcgctgctg ctgctggtga tcccggcgac
     3961 gctgaccgcg ctcggcgtcg tgcgcgagaa ggaactcggc tcgatcacca acctgtacgc
     4021 gagcccggcg acgcggctcg aattcctgct cggcaagcag atgccttacg ccggcctcgg
     4081 actggtcaac gccgcggtga tggcgctgct gaccgtgtgg ctgttcggcg tgccgctgaa
     4141 gggcagcctc cccgccttcg cgctcggggc gctgctgtac gtgatcgcgg cctcggcctt
     4201 cgggctgctg gtctcgacgg tcacgagcac gcagatcgcg gcgctgttcg gcaccttcat
     4261 cctgaccatg ctgccgtcga tcaacttctc gggcctgatc gcgccggtgt cgtcgatgag
     4321 cggcggcgca gcagtgatgg ggcactcctt cccggccagc tacttcctcg cgatcgcggt
     4381 cggaaccttc acgaaggcgc tcggcttcgc ggcgctggca ccgaagtacc tgcagctcac
     4441 cggcttcacg ctggtctacg tgctgctcgc gtggcggctg ctgccgaagc aggagcgctg
     4501 agcgtgcggt cgccaccgct tcgcaacgtg ctcgaactga tgcgccgcga gttctacgcg
     4561 ctcgggcgcg accgcgtcat gctcgggctg atcgtctacg cgttcagcgt gctgatctac
     4621 gtgaatgcca cggccaccgg catcgagctg cgcaacgccg cgatcgcctt cgtcgacgag
     4681 gacgcgagca cgctgtcggc gcggctgttc gatgcgccgc agccgccgta cttccagcac
     4741 ccgcagcgtc tggcactcgc cgatgtcgat gccgcgctcg acgccgggcg cttcaccttc
     4801 gtcgtcgacg tgccgcccgg cttccagggc gacgtgctcg ccggacgcgc gccgcggctg
     4861 cagatcaacg tcgatgccac ggccatgagc caggccggca tcggcgcgaa ctacctgcag
     4921 cagatcttca gcgccgagat cgacgagttc gtgcgcggac gccggcagga ggcgccgcag
     4981 gcggtcacgc aggtcgtgcg catcgccttc aacccgaacc tgcagatgcc gtggttcatg
     5041 gggccgatga tgatcgtcaa cagcatcacg ctgctgtcga tcgtgctgac cggcgcggcg
     5101 ctgatccgcg agcgcgagca gggcacgctc gaacacctgc tggtgctgcc gctgacgccg
     5161 atcgagatca tgctcgcgaa gatcggcgcg acggcgctgg tgatcgtcgt cgccacgacg
     5221 tttgcgaccg tcgtgatgat ccacggcgtg ctgtcggtgc cggcgcgcgg ctcgctcgcg
     5281 ctgttcctgg tcgcggcctt cgtctacagc gcctcgatcg cggcgatcgg catcttcctc
     5341 gcgacgctgg cgcgctcgat gccgcagctc gggctgctgt cgatcccggt ggtgttcccg
     5401 atggcgatgc tctcgggcgg cgccacgccg ctcgacagca tgccggttgc ggtgcagcgg
     5461 atcatgcagt tctcgccgtc gacgcacttc acggcgatcg tgcaggcgat cctgtaccgc
     5521 ggggccggat tcgacgtgat ctggccgcag ttcgtcgcga tgctcgcgat cggtgccgtg
     5581 tttctcgcga tcgcggtgca ccggctcagg acgacgctgg cgcaggcggg aagctgaaac
     5641 gccggatcgt cagcggctgt cccccggcgt ccggagccga tcggcgacca ggccctgcgg
     5701 atgccgtcgc gcggccatcc ggcatgaacc gcgctgccct gccggccgct gccgctcagg
     5761 cgtcgctttc cttctccccg tccagccgtt cgccggtgaa ccggtcgcgc cctgcccagt
     5821 aggtgcagac cgggcaggtc ccgccctcgg gataatgcac gccggcctcg tgcgggcagc
     5881 cgatgatgcg gcccggcgtc aacaccgacc gcaccgcatg gctttcgatg tagcgaacga
     5941 tggcttcgct gatcgccgga tcctggcgga tgtcgccggt ttccgcgtac cagcgctcca
     6001 gcatctcgac ctcggcgttc tcgtccgggg cgatgccgac cacggccttg ctcgccagcc
     6061 ggtcgtcggg gccgtaatag gcgacggtcg cgaccggata gccgtggaaa ccgcggcgga
     6121 acttcttcag cagcttgtcc cgataccaag tggccttgag cgacatcgtg cgtgcctcgt
     6181 cgtgcgggga gggagagcgg gacggtaccc gagccgcccg tcgtcgcgca cgcccgcgct
     6241 ccggctgcgt catcggctga ccgcatgaaa aaggcgcccg caggcgcctt ttcgaaggga
     6301 tcgatcagga tgatcgcgca atcagcgtcc ggcgctggcg cggtaggccg cgtaggggcc
     6361 gcggtgatcg cgggcgcgca gttcgcgcgg catcacgccc tcgaccgagc gcggcacgat
     6421 gccgagcagc gcgaagccgt cctcgccgcc gacgatgctg tcctgctgca gcgagcggta
     6481 gttgtcgagg ctgaagggcc tggtcggcag cttgtccatc agcttcgcct gcagcttcgc
     6541 gaccgaatcc ggcaggccga tgaccagcgt cgacagcccg agcgtgcgcg ccgtgaactc
     6601 gacgagttcc ttcagcgtgt agatgcgcgg gccgccgagt tcgaggcgct tgccgacggt
     6661 gcgctcgtct tcgagggcct gcgcgaaggc cgcggccacg tcgcgcacgt acaccggctg
     6721 gaagcgggct tcggggcagg cgagcggcag caccggcgcg agcttcagca gcccggcgaa
     6781 gcggttgaag aaggaatcct cgcagccgaa gatcaccgag ggccggaagc tcgtgacctt
     6841 caggccggcg gtcgc
//
LOCUS       DAOQIE010000218         6762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5973_length_6762_cov_7.093634, whole genome shotgun sequence.
ACCESSION   DAOQIE010000218 DAOQIE010000000
VERSION     DAOQIE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5973_length_6762_cov_7.093634"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..740)
                     /locus_tag="PK144_13095"
     CDS             complement(6..740)
                     /locus_tag="PK144_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF66708.1"
                     /translation="MARSEPPLRLFLQWLLLNGALLFALWLCWTQGLIATYVLSDPTW
                     ISQAILGLFVAACGHCGLRAWWLARELEQVTAIMRRARAEPDARLQREDAGLRLGDTA
                     LPPSLASEYFTAVLGKYARTAPPESLDHALLTDLLADHASGSHEGGWFLCGLLLKLGL
                     LGTVIGFVMMLGSIATTDSFDVATLQRMLLAMGLGMKVALYTTMIGLIASMLLGAQYL
                     ALDRAADRLVGAIVHFAETELARRAG"
     gene            complement(751..1161)
                     /locus_tag="PK144_13100"
     CDS             complement(751..1161)
                     /locus_tag="PK144_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HNF66709.1"
                     /translation="MPAAPLESFADLVRSALRQKEPHRLLFVFIRVDRPDGAPLPADG
                     GTLSPIMYIDLPPAEELDFATLTRDADAQSPVWDWVMIGALSGTHGTPPTPARAEEVL
                     QTMVRLIEAGGDLSPFVILDRAERPVRLSPAEPV"
     gene            complement(1204..1497)
                     /locus_tag="PK144_13105"
     CDS             complement(1204..1497)
                     /locus_tag="PK144_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66710.1"
                     /translation="MLARSHPLLRLRRGARLRLKCPLPAGFGTWLDAAGRPCAPSPLL
                     LAGWARAGRPWEFVARAPAHALVTPLPDWPGQPRLRLRVPAEAFATLFAAAAA"
     gene            complement(1497..2264)
                     /locus_tag="PK144_13110"
     CDS             complement(1497..2264)
                     /locus_tag="PK144_13110"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNF66711.1"
                     /translation="MPNPTIPDELICRLRHAERVAVLTGAGISAESGVPTFRDAVTGL
                     WAHYRPEDLATPEAYARDPQLVWNWYQWRRGLVAQAQPNAGHMALAQLEARFPAWTLI
                     TQNVDNLHRRAGSRHVVALHGDIARTLCSVEGIEVADWPADGPVPPRCPHCGAGLRPG
                     VVWFGEDLPHAAIDTAFRAAETCDVFLCVGTSTLVYPAAELPFVAHRHRACVVEINPD
                     ETPLSPVADYRLAGPAGVVLPALVEALATADAVAGQA"
     gene            2476..2652
                     /locus_tag="PK144_13115"
     CDS             2476..2652
                     /locus_tag="PK144_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66712.1"
                     /translation="MSHGNPRSASSKSAVAVLRLFAEFAAIILANREKLSGHRPVYRM
                     IDDSELALTIQAAG"
     gene            complement(2672..3760)
                     /locus_tag="PK144_13120"
     CDS             complement(2672..3760)
                     /locus_tag="PK144_13120"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNF66713.1"
                     /translation="MRRRCGLLAACVVIVAAALAAGGGCETVAFYSQAAHGQFEMLAA
                     RRDFAAVANDPQTAPDLRTRLNEIADLRRFAVDVLHLPDDGAYTRYAALQRPAAVWTV
                     VATPPLSLAPRLHCFPIAGCVPYRGWFEAEAADAEAGRLRAAGDDVSLGAVPAYSSLG
                     WFDDPVLDTFVQWPLGRLAELICHELAHRKLYLAGDAAFNEAYATAVGRLGAEAWLRA
                     RGRSDELAAWQAERALQAELRSVLLATRSELATLYDGPLPEAGKRAAKARILAAAQAR
                     YVAVLAARGIAAARPPELNNARLAMLATYEDAVPAFEALFHAGGDDWSTFHAAAAELA
                     TLPPAQRRARLGLPAPEGIATASPDQAR"
     gene            complement(3760..5328)
                     /locus_tag="PK144_13125"
     CDS             complement(3760..5328)
                     /locus_tag="PK144_13125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF66714.1"
                     /translation="MPDTDWKQRYFDVLAEHEQAEAAWKAREEMLGFAVNRLSLLGQG
                     LDARLDPHLESLRAGLRRGDSTERLRAEAGRLADLLDELRRLGHAGRDDELLHLLELL
                     RLPPSRRKALDPLAVRVRQTQPPRGELIEAIAAELNHILLDPSRAPLAAGLPAPGSLA
                     GEDIDGAAPEAEPERSGRTLTDLLAALRLPWTPELELARETLRQRLAQPVPAAAWPQA
                     IEAGAELLNRALAALGGEREELHGFIALVTERLAGLERYVAGERDDLSQARGGSAAFG
                     AAFEAGMHELRSGVGSADGLAALKRTVLGHVERLDHALQSFRAEEEQRLSEFGTRIES
                     LSEQISRLGGETHALRSALVDRDQRLRRDPLTGIGNRRAWDEALAAAVERWLRHGRPL
                     TLALWDIDWFKRINDRLGHAAGDRVLTGVAHLLAESLRAGDTLARYGGEEFALLLPAT
                     PPDEALQVCERMRAAIATSEFAVEGGSVPVTVSCGLTGFRPGDGVRSAFARADAALYA
                     AKRGGRNRCVMAES"
     gene            5743..6741
                     /locus_tag="PK144_13130"
     CDS             5743..6741
                     /locus_tag="PK144_13130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF66715.1"
                     /translation="MTPAISVILPVRDGGDWLREAVDSVRAQSFGDFELLLIDDGSTD
                     DAIARLPPDARLRVLANPGRGLVAALNHGMAQAQGAFLARMDADDRCRPQRFERQLAL
                     LAARPEVAICGVQVELLQPDGGPPPPGGLAYRQWLNALCEPADIARELFIEAPLPHPG
                     WLIRRTAWTRVGPYRDGPWPEDYDWLLRAHLAGLTMAKPADGVLLDWREHPQRLTRRD
                     PRCARANLIAVKAPALAVVLGGREVLLWGATKTGGRLHDALTAQGVRIRAFVDLDPRK
                     RAGRKRDLPVLAPEAAAAHADAAILVTVSQGAKKPAIRTQLAALGRREGIDWWFAT"
BASE COUNT          952 a         2475 c         2492 g          843 t
ORIGIN      
        1 cgtcatcacc cggcgcggcg cgccagttcc gtctcggcga aatgcacgat ggcgccgacc
       61 agccggtcgg cggcgcgatc gagtgcgagg tactgggcgc cgagcagcat cgacgcgatc
      121 aggccgatca tcgtcgtgta cagcgccacc ttcatgccca ggcccatcgc cagcagcatg
      181 cgctgcagcg tcgcgacatc gaaggaatcg gtggtggcga tcgagccgag catcatcacg
      241 aagccgatca cggtgccgag caggccgagc ttcagcagca gcccgcacag gaaccagccg
      301 ccttcgtggc tgcccgaggc gtgatcggcg agcaggtcgg tcagcagcgc gtgatcgagg
      361 gattccggcg gtgcggtgcg ggcgtacttg ccgagcacgg ccgtgaagta ttcgctcgcc
      421 agcgacggcg gcagcgccgt atccccgagt cgcaggccgg catcctcccg ctgcaggcgc
      481 gcgtcgggct cggcccgggc ccggcgcatg atcgccgtga cctgctccag ttcgcgcgcc
      541 agccaccacg cgcgcagccc gcagtgaccg caggcggcga cgaacagccc gaggatcgcc
      601 tgcgagatcc aggtcgggtc cgacagcacg taggtcgcga tcagcccctg cgtccagcac
      661 agccacagcg cgaacagcag cgcgccgttg agcagcagcc actgcaggaa caggcgcagc
      721 ggcggttcgg aacgtgccac ggctcagggc tcagaccggt tcggccggcg acagccgcac
      781 cggccgctcg gcgcgatcga ggatcacgaa cggcgacagg tccccgccgg cctcgatcag
      841 ccgcaccatg gtctgcagca cttcctcggc gcgcgccggc gtcggcggcg tgccatgcgt
      901 gccggacagc gcgccgatca tgacccagtc ccagaccggc gactgcgcat cggcatcgcg
      961 cgtcagcgtc gcgaagtcga gttcctcggc cggcggcagg tcgatgtaca tgatcggact
     1021 gagcgtgccg ccatcggccg gcagcggcgc accgtccgga cggtcgacgc ggatgaacac
     1081 gaacagcagc cggtgcggtt ctttctggcg cagggcgctg cgcaccagat cggcgaagga
     1141 ttccagcggg gcggcgggca tgcggatgac tccttgcagt cgcaggaagg cggaggacgg
     1201 gcttcaggcc gcggcagcgg cgaacagcgt ggcgaaggct tcggccggca cgcgcagccg
     1261 caggcgcggc tgacccggcc agtccggcag cggcgtgacc agcgcatgcg ccggtgcccg
     1321 cgccacgaac tcccagggcc ggccggcgcg cgcccagccg gcgagcagca gcggcgacgg
     1381 cgcacacggc cggccggcgg catcgagcca agtgccgaaa cccgccggca gcgggcattt
     1441 caggcgcagc cgcgcgccgc ggcgcaggcg cagcagcggg tgactgcgcg cgagcatcag
     1501 gcctgccccg ccacggcgtc ggccgtcgcc agcgcctcga ccagcgccgg cagcaccacg
     1561 ccggccggtc cggccagccg gtaatcggcg accggggaca gcggcgtttc gtccgggttg
     1621 atctcgacga cgcaggcccg gtggcggtgc gcgacgaacg gcagttcggc ggccggatag
     1681 acgagggtcg aggtgccgac gcacaggaac acgtcgcagg tctcggcagc gcggaaggcc
     1741 gtgtcgatcg ccgcgtgcgg cagatcctcg ccgaaccaga ccacgcccgg acgcaatccg
     1801 gccccgcagt gcgggcagcg cggcggcacc gggccgtcgg ccggccagtc cgccacctcg
     1861 atgccctcga ccgagcagag cgtgcgcgcg atgtcgccgt gcagcgcgac cacgtggcgg
     1921 ctgccggcgc gccggtgcag gttgtcgacg ttctgcgtga tcagcgtcca ggccggaaag
     1981 cgcgcttcga gctgcgcgag cgccatgtgc ccggcattcg gctgtgcctg cgccaccagc
     2041 ccgcgccgcc actgatacca gttccagacc agctgcggat cgcgtgcgta ggcctcgggc
     2101 gtggccaggt cctcgggccg gtagtgcgcc cagagcccgg tgaccgcatc gcggaaggtc
     2161 ggcacgccgc tctcggcgga gatgccggca ccggtcagca ccgccacacg ctcggcgtgg
     2221 cgcaggcgac agatcagctc gtcggggatc gtggggttcg gcatggacgg tcacgcagcg
     2281 gggggcgact gatcgtagcg ggccgcggcg gcacgcagca aaccgtgtga aggcggggag
     2341 gcaagggttt tcagtgtgcg ctaccgtccg gacctgcagc gagggacatc cgcccgggcc
     2401 gagtcgggtg tatcgcccga acgaaggagt ccggcgtgct cgggcgcatg ggaggctgca
     2461 ccaccgggtg tgccgatgtc ccacggcaat cctcggtcag caagctcaaa atcagccgtt
     2521 gcagtgttgc gcttgttcgc tgaattcgcg gccatcatcc tcgccaaccg cgagaaactg
     2581 tcgggtcatc ggcctgttta tcgaatgatc gacgacagcg agctggcctt gaccattcag
     2641 gctgccggct gaaaggcatt gcgatcgacg ttcagcgcgc ctgatccggc gaagccgtcg
     2701 caattccttc cggcgccggc agccccagtc gcgcccgccg ctgcgccggc ggcagcgtgg
     2761 cgagttcggc tgcggcggcg tggaaggtgg accagtcgtc gccgccggcg tgaaacagtg
     2821 cctcgaaggc cggcaccgcg tcctcgtagg tggcgagcat ggccaggcgg gcgttgttga
     2881 gttcgggcgg gcgggcggcg gcgatgccgc gggcggcgag caccgcgacg tagcgggcct
     2941 gcgcggcggc gaggatgcgc gccttggcgg cgcgcttgcc ggcctcgggc agcgggccgt
     3001 cgtacagcgt cgccagttcg ctgcgcgtgg cgagcagcac gctgcgcagt tcggcctgca
     3061 gcgcgcgctc ggcctgccag gccgcgagtt catcgctgcg cccgcgcgcg cgcaaccagg
     3121 cttcggcccc gagccggccg accgcggtgg cgtaggcctc gttgaacgcg gcatcgccgg
     3181 ccagatacag cttgcggtgc gcaagctcgt ggcagatcag ctcggccagc cgcccgagcg
     3241 gccactgcac gaaggtatcg agcaccgggt cgtcgaacca gccgagcgag gaatacgccg
     3301 gcacggcgcc gagcgagaca tcatcgccgg cggcgcgcag ccgtccggcc tcggcgtcgg
     3361 cggcctcggc ctcgaaccag ccgcggtacg gcacgcagcc ggcgatcggg aagcagtgca
     3421 ggcgcggcgc gagcgacagc ggcggcgtcg cgaccacggt ccacaccgcc gccgggcgct
     3481 gcagcgcggc gtagcgcgtg taggcgccat catcgggcag atgcagcaca tcgacggcga
     3541 agcggcgcag gtcggcgatc tcgttgagcc gcgtgcgcag atccggtgcg gtctgcggat
     3601 cattggcgac cgcggcgaaa tcgcgccgcg ccgccagcat ctcgaactgg ccgtgcgcgg
     3661 cctgcgagta gaacgccacc gtctcgcagc cgccgccggc cgccagcgcc gcggccacga
     3721 tcacgacgca ggccgcgagc agcccgcagc ggcggcgcat catgactctg ccatcacgca
     3781 gcggttgcgc ccgccgcgct tggccgcgta cagcgcggcg tcggcgcgcg cgaaggcgct
     3841 gcgcacgccg tcgccgggcc ggaagccggt cagcccgcac gacaccgtca ccggcacgct
     3901 gccgccctcg acggcaaact cgctggtggc gatggcggcg cgcatgcgct cgcagacctg
     3961 cagcgcctca tcgggcggcg tggccggcag cagcagcgcg aattcctcgc cgccgtagcg
     4021 cgccagcgtg tccccggcac gcaggctttc ggccagcagg tgcgcgacgc cggtcagcac
     4081 gcggtcgccg gcggcgtggc cgagccggtc gttgatgcgc ttgaaccagt cgatgtccca
     4141 cagcgccagc gtcagcggcc ggccgtgacg cagccagcgc tcgacggcgg ccgcgagcgc
     4201 ctcgtcccag gcacggcggt tgccgatgcc ggtcagcggg tcgcggcgca ggcgctggtc
     4261 gcgatcgacc agggcgctgc gcagcgcgtg cgtctcgccg ccgaggcgcg agatctgctc
     4321 ggacaggctc tcgatgcgcg tgccgaactc gctgagccgc tgttcctcct cggcgcggaa
     4381 gctctgcagc gcgtgatcga ggcgctcgac gtggccgagc acggtgcgct tgagcgccgc
     4441 gagtccgtcg gcgctgccga cgccgctgcg cagctcgtgc atgccggcct cgaacgccgc
     4501 gccgaaggcc gcgctgccgc cgcgcgcctg cgagagatcg tcacgctcgc cggcgacgta
     4561 gcgctccagc ccggccagac gctcggtgac cagcgcgatg aaaccgtgca gttcctcacg
     4621 ctcgccgccg agcgcggcca gcgcccggtt cagcagctcg gcgccggcct cgatcgcctg
     4681 cggccaggcc gcggccggca ccggctgcgc gagccgctgg cgcagggtct cgcgcgcgag
     4741 ttcgagttcc ggcgtccacg gcaggcgcag cgccgcgagc agatcggtca gcgtgcggcc
     4801 gctgcgttcc ggctcggctt ccggcgccgc accgtcgatg tcctcgcccg cgaggctgcc
     4861 gggtgccggc aggccggcgg cgagcggggc gcgcgaggga tcgagcagga tgtggttcag
     4921 ctcggcggcg atcgcctcga tcagttcgcc gcgcggcggc tgcgtctggc gcacgcgcac
     4981 ggccagcgga tcgagcgcct tgcgccgcga cggcggcagg cgcagcagtt cgagcaggtg
     5041 cagcagttcg tcatcgcgac cggcatgacc gaggcggcgc agctcgtcga gcagatcggc
     5101 gaggcgcccg gcctcggcgc gcagccgctc ggtgctgtcg ccgcggcgca ggccggcacg
     5161 caggctttcg aggtgcggat cgagccgcgc atccagcccc tgcccgagca gcgacagccg
     5221 gttgaccgcg aagccgagca tctcctcacg cgccttccac gccgcctcgg cctgctcgtg
     5281 ctcggcgagc acatcgaaat aacgctgctt ccagtcggtg tccggcattg cgaaactccg
     5341 ggcgaaaccc agcggggctg gcgatcggac gggcggaacc tgcgtgcgga cgagcctgcg
     5401 cagcgcgccg gcccggccgt ttctccccgg tccgccgggg ggaagggcaa tgcatgacat
     5461 cggaacgtga ccggcacggc ccgccattgc ggccggggcg ccccggcggc caggcggttc
     5521 tgcccttgcg gagtccgcga tccccgacgc aggttccggc gaggacactt gcgggccgcc
     5581 gatgctgcgc cggtccgcga cggccagcaa gcggcaagcg cagcagactt ccggagtgct
     5641 cttgcaagac cctgcccccc tcgcctttcc cgccccgaac cgtgcagccg cccgtggatt
     5701 gcgccgcccc gcgcgccgca ttcacccctc gctcaaccgc cgatgacgcc cgcgatttcg
     5761 gtgatcctgc cggtgcgcga cggcggcgac tggctgcgcg aggccgtcga cagcgtgcga
     5821 gcacagagct tcggcgactt cgagctgctg ctgatcgacg acggctcgac cgacgacgcc
     5881 atcgcccgcc tgccgccgga tgcgcgcctg cgcgtgctcg ccaaccccgg ccgcgggctg
     5941 gtcgcggcgc tcaaccacgg catggcgcag gcgcaaggcg cgtttctggc gcgcatggac
     6001 gccgacgacc gctgccggcc gcagcgcttc gagcgccagc tcgcgctgct ggcggcgcgc
     6061 ccggaggtgg cgatctgcgg cgtgcaggtc gagctgctgc agcccgacgg cggtccgccg
     6121 cctccgggcg gcctcgcgta ccggcaatgg ctgaacgcgc tctgcgagcc ggccgacatc
     6181 gcccgcgagc tgttcatcga agcgccgctg ccgcatcccg gctggctgat ccggcgcacg
     6241 gcctggacgc gggtcgggcc gtatcgcgat ggcccgtggc cggaggatta cgactggctg
     6301 ctgcgcgccc acctggccgg cctgacgatg gccaagcccg ccgatggcgt gctgctcgac
     6361 tggcgcgaac acccgcagcg cctgacccgc cgtgacccgc gctgcgcccg cgcgaacctg
     6421 atcgcggtga aagccccggc gctcgccgtc gtgctcggcg ggcgcgaggt gctgctgtgg
     6481 ggcgcgacga agaccggcgg ccgcctgcac gacgcgctca cggcgcaggg cgtgcgcatc
     6541 cgcgccttcg tcgatctcga cccgcgcaag cgcgccgggc gcaagcgcga cctgccggtg
     6601 ctggcccccg aggccgccgc cgcccacgcc gacgccgcga tcctcgtgac cgtctcgcag
     6661 ggcgcgaaga agccggcaat ccgcacccag ctcgccgcgc tcggccggcg cgagggcatt
     6721 gactggtggt ttgcgacctg agcggccggc acactccgcg cc
//
LOCUS       DAOQIE010000219         6757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_5983_length_6757_cov_7.670845, whole genome shotgun sequence.
ACCESSION   DAOQIE010000219 DAOQIE010000000
VERSION     DAOQIE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5983_length_6757_cov_7.670845"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2742
                     /locus_tag="PK144_13135"
     CDS             <1..>2742
                     /locus_tag="PK144_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNF66716.1"
                     /translation="ELDLLYKDLLIGVTRFFRDPEAFEILRTRVVPGIVERAPEDEDV
                     RVWVCGCSTGEEAYSLAILFLEAFAAAGRAPRIKILATDIHRKSLLTAGEGVYPESSL
                     TEMPEALRQKYFIEQPDRTWRVTANLRKALIFSEHNLLKDPPFTRIDLVSCRNLLIYL
                     EPPAQLRAIAAFHFALRVEGCLYLGASEGLGELAIEFGIVDRHWKIFTKLRDNRLLHD
                     LRLPLGYSTKSSLKALPGIGETRLGRIYDVLLTQFIPTGILVNDRYEAVHVFGDASRY
                     LLPPSGKVTSDLFSMVGGHLRIALMTALRNAQQQNSAVHYRGIVHESSGGERRFLNVG
                     VQPLHDTTLNTRYYMVRIDEAASLPVSSSGTAADVQVAGRPLDVDAQTYAQIRVLELE
                     LQQARESLQSTIEELETSNEELQATNEELLASNEELQSTNEELHSVNEELYSVNAEHE
                     LKIQELNAVTSDLNNLISSTELATLFIGADFRLRLFTPQATTIFPLLPQDIGRDLRHF
                     KPVETDEHLLDDVRQVLKTHAAVERTLAWSRERTFLRRVTTYQDTNKAIAGVVLTYVD
                     ISETARLGRELDRQRLRLEAVVDTSPNGLLIVDAEGRITMANPALARIFGYSHGALDG
                     HRVEDLLPPEQREAHIALRASYLRAPMTRAMGQGRELLGLRRDGSRFPVEVSLAAFRT
                     DDGDFVQATVIDISERRRAEETVRVAQLENARLAALIEATGDAIIGTDELGAITSWNP
                     GARALLGHAPATVLGQPLASLFPDGDTGGLAMIAEIHGSQSLRRDCVLRHADGHDVDV
                     AVTISPVRSAAGRLLGLAVVAHEAGERIRRAAEREREREQLEREVEARTQELRTLNVQ
                     LGEALASAEAATRAKSAFLANMSHEIRTPLNAITGMAYLLKESITDAGQRSQINKIER
                     "
     assembly_gap    2743..2842
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2843..3538
                     /locus_tag="PK144_13140"
     CDS             <2843..3538
                     /locus_tag="PK144_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66717.1"
                     /translation="RACVRFEVEDTGIGIDPADAARLFNAFEQADTSTTRRYGGTGLG
                     LAITRGLAALMGGTVGVDARRGGGSVFWFTAWLAKAPAAAAGTAPALLLVKGAREDLH
                     AQFGGARVLLAEDDPVNREVAAELLRMADLDVDCAINGVEAIACVQSRRYDLILMDMQ
                     MPEIDGLDATRRIRDLANGRDVPILALTANAYNEDRERCLAAGMDDFVSKPIEAPRLY
                     ANVLVWLKQGRRR"
     gene            complement(3576..5009)
                     /gene="xseA"
                     /locus_tag="PK144_13145"
     CDS             complement(3576..5009)
                     /gene="xseA"
                     /locus_tag="PK144_13145"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNF66718.1"
                     /translation="MNEPVVPARDIVSVSALVRQARGLLEMNLPMLWVEGEISNLARP
                     ASGHLYFTLKDEFAQVRCAMFRTRALLLREAPANGRQVLVRARVTLYEARGEFQLVVE
                     SLEPAGEGRLRRAFEALRARLEAEGLFDAAHKRAIPRLAQRIGVLTSPAGAALRDVLT
                     TLARRAPQIPVIVYPVPVQGDGAAAKIIAMLKTAAARAECDVLLLVRGGGSLEDLQAF
                     NDEALARAVRACPIPVIAGIGHETDVSITDLVADLRAATPTAAAVAASPDTADWRERT
                     TRLTLRLHGALQRRRQRLAQLLQSLRARLDRQQPRRRLEAHAQRLDELDGRLRRALAA
                     HLRQRRQRQAQLLMRLAAQSPRRRLAEQRRRLKTAQGRLEPAALRRRLQGLAGHHEAL
                     VRRLAAAMRHRLAVQQARYAALLRELHALSPLATLARGYAIVRDAEGRALRHAGDVAE
                     GSRITAQLAHGRLHCRVEPAGDDEAGL"
     gene            5195..6658
                     /gene="guaB"
                     /locus_tag="PK144_13150"
     CDS             5195..6658
                     /gene="guaB"
                     /locus_tag="PK144_13150"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNF66719.1"
                     /translation="MRIVQEALTFDDVLLLPAHSTVLPKDVSLATRLTREITLNLPLV
                     SAAMDTVTESRLAIAMAQEGGIGIVHKNMTAEQQAAEVRRVKKFEAGVISDPITVSPR
                     LSIREVLAITRANGISGVPVVDGEDLVGIVTSRDLRFETNFDAPVSRIMTPKERLVTV
                     REGASREEVIALLHRHRIEKVLVVNDRFQLRGMITVKDIQKSSDFPNACKDSRGRLRV
                     GAAVGTGAGTDERVTALVGAGVDVIVVDTAHGHSQGVLERVRWVKSQFPDVQVIGGNI
                     ATADAAIALAEAGADAVKVGIGPGSICTTRIVAGVGVPQISAVANVAAALKTAGIPLI
                     ADGGIRYSGDLAKAIVAGAYAVMVGGLLAGTEEAPGEVELFQGRSYKAYRGMGSLGAM
                     AGSQGSADRYFQDASAVEKLVPEGIEGRVPYKGPMSAIVHQLMGGLRSSMGYVGCATL
                     DDMRTRPEFVRITGAGMRESHVHDVQITKEAPNYRVS"
BASE COUNT         1004 a         2416 c         2332 g          905 t
ORIGIN      
        1 cgagctcgac ctgctgtaca aggacctgct gatcggcgtc acgcgcttct tccgcgaccc
       61 cgaggccttc gagatcctgc gcacgcgcgt cgtgcccggc atcgtcgagc gcgctcccga
      121 ggacgaggac gtgcgcgtct gggtctgcgg ctgctcgacc ggcgaggaag cctactcgct
      181 ggcgatcctg ttcctcgaag ccttcgcggc ggccgggcgc gcgccgcgca tcaagatcct
      241 cgccaccgac atccaccgca agtcgctgct gaccgccggc gagggcgtct acccggagag
      301 cagcctgacc gagatgccgg aggcgctgcg ccagaagtac ttcatcgagc agcccgaccg
      361 cacctggcgc gtgaccgcga acctgcgcaa ggcgctgatc ttctccgagc acaacctgct
      421 gaaggacccg ccgttcacgc gcatcgacct ggtcagctgc cgcaacctgc tgatctacct
      481 cgaaccgccg gcgcagctgc gcgcgatcgc ggccttccac ttcgcgctgc gcgtcgaggg
      541 ctgcctgtac ctcggcgcga gcgaggggct cggcgagctg gccatcgagt tcggcatcgt
      601 cgaccggcac tggaagatct tcacgaagct gcgcgacaac cgcctgctgc acgacctgcg
      661 cctgccgctc ggctacagca ccaagagttc gctgaaggcc ctgcccggca tcggcgagac
      721 ccggctcgga cgcatctacg acgtgctgct gacgcagttc atcccgaccg gcatcctcgt
      781 caatgaccgc tacgaggccg tgcacgtgtt cggcgatgcc tcgcgctacc tgctgccgcc
      841 ctcgggcaag gtcaccagcg acctgttctc gatggtcggc gggcacctgc gcatcgcgct
      901 gatgacggcg ctgcgcaacg cccagcagca gaactcggcc gtgcactacc gcggcatcgt
      961 ccacgagagc agcggcggcg agcggcgctt cctgaacgtc ggcgtgcagc cgctgcacga
     1021 cacgacgctg aacacgcgct actacatggt gcgcatcgac gaggccgcca gcctgccggt
     1081 cagcagctcg ggaacggccg ccgatgtgca ggtcgccggc cgtccgctcg atgtcgatgc
     1141 gcagacctac gcgcagatcc gcgtgctcga actcgaactg cagcaggcgc gcgagtcgct
     1201 gcagagcacg atcgaggaac tcgagaccag caacgaggaa ctgcaggcca ccaacgagga
     1261 actgctggcc tccaacgagg agctgcagag caccaacgag gagctgcact cggtcaacga
     1321 ggagctctac agcgtcaacg ccgagcacga gctgaagatc caggagctca acgccgtcac
     1381 cagcgacctg aacaacctga tcagctcgac cgagctggcg acgctgttca tcggtgccga
     1441 cttccggctg cggctgttca cgccgcaggc gaccacgatc ttcccgctgc tgccgcagga
     1501 catcggccgc gacctgcgcc atttcaagcc ggtggagacc gacgagcacc tgctcgatga
     1561 cgtgcgccag gtgctcaaga cccacgccgc cgtcgagcgc acgctggcct ggagccgcga
     1621 gcgcaccttc ctgcgtcgcg tcacgaccta tcaggacacc aacaaggcca tcgccggcgt
     1681 ggtgctgacc tacgtcgaca tcagcgagac ggcccgcctc gggcgcgaac tcgaccgcca
     1741 gcgcctgcgg ctcgaagccg tcgtcgacac cagcccgaac ggtctgctga tcgtcgatgc
     1801 cgagggccgc atcacgatgg ccaacccggc gctggcgcgc atcttcggct actcgcacgg
     1861 tgcgctcgac ggccatcggg tcgaggacct gctgccgccc gagcagcgcg aggcccacat
     1921 tgccctgcgc gcgagctacc tgcgcgcccc gatgacgcgg gcgatggggc aggggcgcga
     1981 gttgctcggc ctgcgccgcg acggcagccg cttcccggtc gaggtcagcc tcgcggcctt
     2041 ccgcaccgac gacggcgact tcgtgcaggc caccgtcatc gacatcagcg agcgccgccg
     2101 cgccgaggaa accgtccgcg tcgcgcagct cgagaacgcc cggctcgcgg cgctgatcga
     2161 ggccaccggc gacgcgatca tcggcaccga cgaactcggc gcgatcacga gctggaatcc
     2221 cggcgcccgc gcgctgctcg gccatgcacc ggcgacggtg ctcggccagc ctctggcctc
     2281 gctgttcccg gatggcgaca ccggggggct ggccatgatc gccgagattc acggcagcca
     2341 gtcgctgcgc cgcgactgcg tgctgcgcca cgccgacggc cacgatgtcg atgtggcggt
     2401 gacgatctcg ccggtgcgca gtgccgcagg ccggctgctc ggcctggccg tggtcgcgca
     2461 cgaggccggc gagcgcatcc gccgcgccgc cgagcgcgag cgcgagcgcg agcagctcga
     2521 acgcgaggtc gaggcgcgca cgcaggagct gcgcaccctc aacgtccagc tcggcgaggc
     2581 gctcgccagt gccgaggccg cgacccgggc caagagcgcc ttcctcgcca acatgagcca
     2641 cgagatccgc acgccgctga acgcgatcac cggcatggcc tacctgctca aggagagcat
     2701 caccgacgcc ggccagcggt cgcagatcaa caagatcgag cgnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nncgcgcctg cgtgcgcttc gaggtcgagg acaccggcat
     2881 cggcatcgat ccggccgacg cggcgcggct gttcaacgcc ttcgagcagg ccgacacctc
     2941 gaccacgcgc cgctacggcg gcaccggcct cgggctggcg atcacgcgcg ggctggcggc
     3001 gctgatgggc ggcacggtcg gcgtcgatgc ccgccgtggc ggcggcagcg tgttctggtt
     3061 cacggcctgg ctggccaagg cgccggccgc cgcggccggc acggctccgg ccctgctgct
     3121 ggtcaagggc gcgcgcgagg acctgcacgc gcagttcggc ggtgcgcgcg tgctgctggc
     3181 cgaggacgat ccggtcaacc gcgaggtggc ggccgaactg ctgcgcatgg ccgatctcga
     3241 tgtcgactgc gcgatcaacg gcgtcgaggc gatcgcctgc gtgcagtcgc gccgctacga
     3301 cctgatcctg atggacatgc agatgccgga aatcgacggt ctggacgcga cccgccgcat
     3361 ccgcgacctc gccaacggcc gcgacgtgcc gatcctggcg ctgaccgcca acgcctacaa
     3421 cgaggaccgc gaacgctgcc tcgccgccgg catggacgac ttcgtctcca agccgatcga
     3481 ggccccgcgg ctgtacgcga acgtgctggt gtggctgaag caggggcggc ggcgctagcc
     3541 gggcgcggcg ctgcgcagtc ccccggaagc cggcttcaga ggccggcttc gtcgtctccg
     3601 gccggctcga cgcggcagtg cagccgtccg tgcgcgagct gcgcggtgat cctgctgccc
     3661 tcggccacgt cgccggcatg gcgcagcgcg cggccctcgg cgtcgcgcac gatcgcgtag
     3721 ccgcgcgcga gtgtggccag cggactgagc gcatgcagtt cgcgcagcag cgcggcgtaa
     3781 cgcgcctgct gcacagcgag ccggtgccgc atcgccgcgg ccagccgccg gaccagggcc
     3841 tcgtggtgcc cggccagtcc ctgcaggcgc cggcgcaggg ctgccggctc caggcgtccc
     3901 tgcgccgtct tcagccgccg gcgctgttcg gccagccgcc ggcgcggcga ctgcgcggcg
     3961 aggcgcatca gcagttgcgc ttgccgctgc cggcgctggc gcaggtgtgc cgccagcgcc
     4021 cggcgcagcc ggccgtcgag ctcgtcgagg cgctgcgcat gcgcttcgag ccgccgccgc
     4081 ggctgctggc gatcgaggcg tgcgcgcagg gactgcagga gctgcgcgag gcgctggcgg
     4141 cggcgctgca gggcgccgtg caggcgcagc gtcaggcgcg tggtgcgctc gcgccagtcg
     4201 gcggtgtcgg ggctggcggc gaccgcggcg gcggtcgggg tcgcggcgcg caggtcggcg
     4261 accagatcgg tgatgctcac gtcggtctcg tggccgatgc cggcgatcac cgggatcggg
     4321 caggcgcgca cggcccgcgc cagcgcctcg tcattgaagg cctgcaggtc ttcgagcgag
     4381 ccgccgccgc gcacgagcag cagcacgtcg cactcggcgc gcgcagcggc ggtcttcagc
     4441 atcgcgatga tcttcgcggc ggcgccatcg ccctgcaccg gcaccggata gacgatgacc
     4501 gggatctgcg gcgcgcgccg tgcaagcgtc gtcagcacgt cgcgcagcgc cgcgccggcc
     4561 ggcgaggtga ggacgccgat gcgctgcgcc agccgcggaa tcgcgcgctt gtgtgcggca
     4621 tcgaacaggc cctcggcttc gaggcgcgcg cgcagcgcct cgaaggcgcg gcgcaggcgg
     4681 ccctcgccgg ccggttcgag cgattcgacc acgagctgga actcgccgcg cgcctcgtac
     4741 agcgtcacgc gcgcacgcac cagcacctgc cggccgttgg cgggtgcctc gcgcagcagc
     4801 agcgcgcggg tgcggaacat cgcgcagcgc acctgcgcga actcgtcctt cagcgtgaag
     4861 tacagatgcc ccgaggccgg gcgcgcgagg ttggagatct cgccctcgac ccacagcatc
     4921 ggcaggttca tctccagcag gccgcgggcc tgacgcacca gcgccgagac gctgacgatg
     4981 tcgcgggcgg gcacgacggg ttcgttcatc ggcggcaggc tccgggccgg gtgggcgtga
     5041 ggtgtgggca gggcggggcg tgcagcctgc cacagccatg tcgtgctgca ccgcagccaa
     5101 atgcggacga ccggacgcgc cggcctgttc cgcccttgcg tcagaccgtt atacttcgcc
     5161 gcttccttat cgccggctca ggattccccc catgctgcgt atcgttcagg aagcactcac
     5221 cttcgatgac gtgctgctgc tgcccgcgca ttccacggtc ctgcccaagg acgtgtcgct
     5281 cgccacgcgc ctgacgcgcg agatcacgct gaacctgccg ctggtctcgg cggcgatgga
     5341 cacggtgacg gaatcccggc tggcgatcgc gatggcgcag gaaggcggca tcggcatcgt
     5401 gcacaagaac atgaccgccg agcagcaggc cgccgaggtg cgccgggtca agaagttcga
     5461 ggccggcgtc atctccgatc cgatcaccgt ctcgccgcgg ctgagcatcc gcgaggtgct
     5521 ggcgatcacg cgcgccaacg gcatctccgg cgtgccggtg gtcgatggcg aggatctggt
     5581 cggcatcgtc accagccgcg acctgcgctt cgagaccaac ttcgatgcgc cggtcagccg
     5641 catcatgacg ccgaaggagc gtctggtgac cgtgcgcgag ggcgcctcgc gcgaggaagt
     5701 catcgcgctg ctgcaccgcc accgcatcga gaaggtgctg gtcgtcaacg accgcttcca
     5761 gctgcgcggc atgatcaccg tgaaggacat ccagaagtcc tcggacttcc cgaacgcctg
     5821 caaggacagc cggggccggc tgcgcgtcgg cgccgcggtc ggcaccggtg ccggcaccga
     5881 cgagcgggtc acggcgctgg tcggtgccgg ggtcgacgtg atcgtcgtcg acaccgcgca
     5941 cggccactcg cagggcgtgc tcgagcgcgt gcgctgggtc aagtcgcagt tcccggacgt
     6001 gcaggtcatc ggcggcaaca tcgccacggc cgatgccgcg atcgcgctcg ccgaggccgg
     6061 cgccgatgcg gtgaaggtcg gcatcggtcc gggttcgatc tgcacgacgc gcatcgtcgc
     6121 cggcgtcggc gtgccgcaga tcagcgccgt cgccaatgtc gccgcggcgc tgaagaccgc
     6181 cggcatcccg ctgatcgccg atggcggcat ccgctattcc ggcgatctcg ccaaggccat
     6241 cgtcgccggc gcctacgcgg tcatggtcgg cggcctgctc gccggcaccg aggaagcccc
     6301 gggcgaggtc gagctgttcc agggccgctc gtacaaggcc taccgcggca tgggttcgct
     6361 cggcgcgatg gccggttcgc agggttcggc cgatcgctac ttccaggacg ccagcgccgt
     6421 cgagaagctc gtgcccgagg gcatcgaggg ccgcgtgccg tacaaggggc cgatgtcggc
     6481 gatcgtgcac cagctgatgg gcggcctgcg ttcgagcatg ggctacgtcg gctgcgcgac
     6541 gctcgacgac atgcgcaccc gccccgagtt cgtgcgcatc accggcgccg gcatgcgcga
     6601 atcgcacgtg cacgacgtgc agatcaccaa ggaagccccg aactaccgcg tcagctgagc
     6661 cgcccgcggc ttgcggcacg cactgcccgg caaagaagca cgccgccgcc cgagcggcac
     6721 aacccgaacg gcgaggggtg gcaggatcac ccctcgc
//
LOCUS       DAOQIE010000220         6746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6000_length_6746_cov_6.923180, whole genome shotgun sequence.
ACCESSION   DAOQIE010000220 DAOQIE010000000
VERSION     DAOQIE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_6746_cov_6.923180"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="PK144_13155"
     CDS             complement(<1..1504)
                     /locus_tag="PK144_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNF66720.1"
                     /translation="MNLSQTIATELAVRPAQVEAAVRLLDEGATVPFIARYRKEVTGG
                     LDDTQLRTLEERLGYLRELEDRRAAVLASIGEQGRLTDALRADILAADTKTRLEDLYL
                     PYKPKRRTKAQIAREAGLAPLAETLLADPQQAPEALAAGFVAPDRGVADVKAALDGAR
                     QILMETFAEDAELLGRLRERLWSEGVLASRLVDGRQDEGAKFSDYFDFAEPLHKVPSH
                     RALALLRGRNEGVLTLGLDLPVAEGQPHPAEAMIAARFGVRALGRPADAWLTESVRWA
                     WKIKLSSALDTELKNRLREAAETEAIRVFATNLKDLLLAAPAGPRAVLGLDPGLRTGC
                     KVAVVDATGKLLATETIHPHVPHQRWDEALAKLAVLCRTHAVALVAIGNGTASRETDR
                     LVAELIRREPGLGLGKIVVSEAGASVYSASELAALEFPGLDVSLRGAVSIARRLQDPL
                     AELVKIEPKAIGVGQYQHDVNQTQLARSLDAVVEDCVNAVGVDVNTASVPL"
     gene            complement(1600..2079)
                     /gene="rraA"
                     /locus_tag="PK144_13160"
     CDS             complement(1600..2079)
                     /gene="rraA"
                     /locus_tag="PK144_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNF66721.1"
                     /translation="MGFKTADLCDEHADRLQIAEPVFGDYGGEISFFGPITTLKAFED
                     NSLVRKAVEEKGEGRVLVIDSGGSMRCAMVGDQLAALAEKNGWAGVIVNGCIRDSAAI
                     ADTEIGCKALGVHPLKTVKRNEGQRDVTLRFAGVSFVPGHYLYADEDGLVVSEQPLF"
     gene            2224..2685
                     /locus_tag="PK144_13165"
     CDS             2224..2685
                     /locus_tag="PK144_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HNF66722.1"
                     /translation="MWLEREDFRTAVRLSPLVSIDLVVRDAAGRVLLGWRNNRPAQHC
                     WFVPGGSIRKQETLDAAFRRISHDELGLGCERAEARWLGVYEHFYDDHFAGTDFGTHY
                     VVLGHELAVGSGLTTLPDAQHAHWRWFEVDELLASPEVHRHSKWYFDPRAC"
     gene            2831..3604
                     /locus_tag="PK144_13170"
     CDS             2831..3604
                     /locus_tag="PK144_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF66723.1"
                     /translation="MNELAGFLVGTVVGFTGVGGGALMTPILLFFFGVAPQTAVGTDL
                     LFAAITKFAGSLVHGSRGSIDWLVLRRLCYGSLPAAALTLYVLHGQRIDGEVQTVILR
                     TLGIALALTSLSVLFKNQMHLIGRKLRIGRPERFKRFQGPLTIFAGTILGALVTLTSV
                     GAGALGAVMLLYLYPLRLTPAKLVGTDLAHAIPLTLVAGLGHLSLGNIDTTLLGGLLT
                     GSIPGVLLGSWLSHRLDGAWVARGIAVLLLISGGKLLAI"
     gene            complement(3612..5033)
                     /locus_tag="PK144_13175"
     CDS             complement(3612..5033)
                     /locus_tag="PK144_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF66724.1"
                     /translation="MRRRRTEAAGGYNVWPVFTDALSGLVTVLVFLVTVFVIGETWLA
                     REVSGRDDAIGELRVQIARLQTQLGLSERRAQVAETRAAEADATIGERELDLADLRAA
                     LAQADATRLRLGAELERSRHALDEQKARQLQAQDEADRQARALGGERDALLARAAAQA
                     QALDAGERQRLAAEAGIAGLNLRLSRLQGDLERLQQALALAEDQRRQDAEAHAAALAT
                     ERRQAAATLAEAHTQAAATLGVEHQRAAVERETAAAQLAALEAEKARLERERAALAGR
                     LDEQATALAATQTKLAERDATVTEQAERLDELDRQVRLRLVERVEQLSRYASEFFGRL
                     RTLFEDDPDIRVVGDRFVFQSEVLFGSGEAALSEAGKPDLDKFAGVYRQLSGRLPEDL
                     PVIIEIIGHTDREPIRASRFRSNWELSTARAQAVVDYLIRQGIPPQRLAAVGMGEFHP
                     VDPADTPEAFRRNRRIELKITSR"
     gene            complement(5014..6084)
                     /locus_tag="PK144_13180"
     CDS             complement(5014..6084)
                     /locus_tag="PK144_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66725.1"
                     /translation="MSAPAGAGVDRFLVRAGLFLLLLAGLALLLRAPLANAFRISPYL
                     NGLILVLFLFGAGYTLRCLGLLRREQAACARVARLAAEVRAGTRAAGEAAELMTLPSL
                     GAVGEFLARVRLTLRQADAQQTLPFLLETLAGRGEDARALVRFLGGTLVLIGLIGTFY
                     GLLLSTEGVRDLLGTAAGGDGADWLANLRARLATPLTGMGTGFATSLFGLVCSAALGF
                     LELQLFHAQSAVTTRLETLVVTELLPVWQTPALIGREQPVAPRYLLAVAEALIERLDR
                     VAGLLEAAAARDDAGRLAQAVARGDERVQALGVRLEREAREQNAALMHELRVIARYMA
                     RDTQPPAVSPQTAEPHDAPPSH"
     gene            complement(6095..6712)
                     /locus_tag="PK144_13185"
     CDS             complement(6095..6712)
                     /locus_tag="PK144_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNF66726.1"
                     /translation="MPHPLPGKPAVTTVAIDAALARRWSPRALDPARPLARAHLQALL
                     EAARWAPSCFGDQPWRFVCWDRSRDPAGFAAALATLSEGNRVWAQHAAVLLLAVACAD
                     FRHNGQPNRWAQYDTGAASENLCIQAAALGLAAHQMGGFDAAAVTKLAGLPERATPMA
                     MIAIGHPAPADVLPEALRTRETAARTRLALSGIAFEGGWAQPLGD"
BASE COUNT          964 a         2402 c         2384 g          996 t
ORIGIN      
        1 gcagcggcac cgaggcggtg ttgacatcga cgccgacggc gttgacgcag tcctcgacca
       61 cggcatcgag cgaacgcgcg agctgcgtct ggttcacgtc atgctggtac tggccgacgc
      121 cgatcgcctt cggctcgatc ttgaccagtt cggcgagcgg gtcctgcagc cggcgcgcga
      181 tcgacaccgc gccgcgcagc gacacgtcga gccccgggaa ttccagcgcc gcgagttcgg
      241 aggccgaata caccgaagca ccggcctcgg agacgacgat cttgccgagc ccgaggccgg
      301 gctcgcgccg gatcagctcg gcgaccagcc ggtcggtctc gcgcgaggcc gtgccgttgc
      361 cgatcgccac cagcgccacg gcgtgcgtgc ggcaaaggac tgcgagcttc gcgagcgcct
      421 cgtcccagcg ctggtgcggc acgtgcgggt ggatggtctc ggtcgcgagc agcttgccgg
      481 tggcatcgac gaccgccacc ttgcagccgg tgcgcaggcc cggatcgagg ccgagcacgg
      541 cgcgcggacc ggccggcgcg gcgagcagca ggtccttcag gttggtcgcg aacacgcgga
      601 tcgcctcggt ctcggcggcc tcgcgcaggc ggttcttcag ttcggtgtcg agcgcgctcg
      661 agagcttgat cttccaggcc cagcgcacgc tttcggtgag ccaggcatcg gccgggcggc
      721 cgagcgcgcg cacgccgaag cgcgccgcga tcatggcttc ggccgggtgc ggctggcctt
      781 cggcgaccgg caggtcgagg ccgagcgtca gcacgccctc gttgcgcccg cgcagcagcg
      841 ccagcgcgcg gtgcgacggc accttgtgca gcggctcggc gaagtcgaag tagtccgaga
      901 acttcgcacc ctcgtcctgc ctgccatcga ccaggcggct tgccagcacg ccttcgctcc
      961 agagccgctc gcgcagccgg ccgagcagtt cggcgtcctc ggcgaaggtt tccatcagga
     1021 tctggcgggc accgtcgagc gcggccttca cgtcggcgac gccgcgatcc ggcgcgacga
     1081 agccggcggc cagggcttcc ggcgcctgct gcggatcggc gagcagggtt tcggcgagcg
     1141 gcgcaagccc cgcctcgcgg gcgatctgcg ccttggtgcg gcgcttgggc ttgtacggca
     1201 ggtacaggtc ttcgagccgg gtcttggtgt cggcagcgag gatgtcggcc cgcagcgcgt
     1261 cggtcagcct gccctgctcg ccgatgctgg cgagcacggc ggcgcggcgg tcttccagct
     1321 cgcgcaggta gccgagacgt tcctcgagcg tgcgcagctg cgtgtcgtcg aggccgccgg
     1381 tgacttcctt gcggtagcgg gcgatgaacg gcacggtcgc gccctcgtca agcaggcgca
     1441 cggcggcctc cacctgcgcg ggccgcacgg ccagttcggt ggcgatggtc tgggagaggt
     1501 tcatggcggg cggcaaaccg gcggttccgg gggcgatggc tccggaaccg gcgctggggg
     1561 cgatgcggcg gagtcaggaa aaaaggacag cggcccgcct cagaacagcg gctgttccga
     1621 gacgaccaga ccgtcctcgt cggcatagag gtagtgtccg ggcacgaagc tgacgccggc
     1681 gaagcgcagc gtcacgtcgc gctggccctc gttgcgcttg acggtcttca gcgggtgcac
     1741 gccgagcgcc ttgcagccga tctcggtatc cgcaatggct gccgagtcgc ggatgcagcc
     1801 gttgacgatg acaccggccc agccgttctt ctcggccagc gccgcgagct ggtcgccgac
     1861 catcgcgcag cgcatcgagc cgccgctgtc gatgaccagc acccggccct cgcctttttc
     1921 ctcgacggcc ttgcgcacca gcgagttgtc ctcgaaggcc ttcagcgtcg tgatcggacc
     1981 gaagaagctg atctcgccgc cgtagtcacc gaacaccggt tcggcgatct gcaggcggtc
     2041 ggcgtgttcg tcacagaggt cggcagtctt gaagcccatc ggtcggggtc tcctgcgagc
     2101 gggtctgaag cggtgatgga aatgcaaatc acgctggcga actgcggcac cggcaaggcg
     2161 cgcgagtgta gcatgcggca gtgcggcaaa ccggccgcgc ctgccccccg gaagtctgga
     2221 cgcatgtggc tcgaacgcga ggatttcagg accgccgtgc gcctgtcgcc gctggtgtcg
     2281 atcgatctgg tcgtgcgcga tgccgccggc cgcgtgctgc tcggctggcg caacaaccgc
     2341 ccggcgcagc actgctggtt cgtgccgggc ggcagcatcc gcaagcagga aaccctggac
     2401 gcggcgttcc ggcgcatcag ccatgacgaa ctcggcctgg gctgcgaacg cgccgaagcg
     2461 cgctggctcg gcgtctacga acacttctac gacgatcact ttgccggcac cgacttcggc
     2521 acccattacg tcgtgctcgg ccacgagctg gcggtcggct cggggctgac gacgctgccc
     2581 gatgcccagc acgcgcactg gcgctggttc gaagtcgacg aactgctcgc cagccccgag
     2641 gtgcaccggc attcgaagtg gtacttcgat ccgcgggcct gctgaaaaac cgggcacgat
     2701 ggatataact tttaatcatt aaaacaaaat tattcataat gtaataatga aacgtatcgc
     2761 ccggatgccg cacaccggca gcggcacggg cctgcgatcc cgatttcaat tgcttcagga
     2821 atgcttgacc atgaatgaac tggccggttt tctggtcggc acggtggtgg gattcaccgg
     2881 tgtcggcggc ggtgccctga tgacgccgat cctgctcttc ttctttggcg tggccccgca
     2941 gaccgcggtc ggcaccgacc tgctgtttgc ggcgatcacc aagttcgccg gctcgctcgt
     3001 ccatggctcg cgcggctcga tcgactggct ggtgctgcgc cggctctgct acggcagcct
     3061 gccggccgcg gccctgacgc tgtacgtgct gcacggtcag cggatcgacg gcgaggtgca
     3121 gacggtgatc ctgcgcacgc tcggcatcgc gctggcgctg accagcctgt cggtgctgtt
     3181 caagaaccag atgcacctga tcgggcgcaa gctgcgcatc ggccggccgg aacgcttcaa
     3241 gcgcttccag gggccgctga caattttcgc cggcaccatc ctcggtgcgc tggtgacgct
     3301 gacctcggtc ggtgccggcg cgctcggggc cgtgatgctg ctgtacctct atccgctgcg
     3361 cctgacgccg gcgaagctgg tcggcaccga tctcgcgcac gcgattccgc tgacgctggt
     3421 cgccggcctc ggtcacctgt cgctcggcaa catcgacacc accctgctcg gcggcctgct
     3481 gaccggctcg attcccggcg tgctgctcgg ctcctggctc agtcaccggc tcgacggcgc
     3541 ctgggtcgcg cgcggcatcg ccgtgctgct gctgatcagc ggcggcaagc tgctggcgat
     3601 ctgaggcggc ctcagcggct ggtgatcttc agctcgatgc gccggttgcg ccggaaggcc
     3661 tccggcgtgt cggcgggatc gaccggatgg aactcgccca tgccgaccgc cgccagccgc
     3721 tgcggcggga tgccctgacg gatcaggtag tcaacgaccg cctgcgcgcg cgccgtcgac
     3781 agctcccagt tcgagcggaa gcggctggcg cggatcggct cgcggtcggt gtggccgatg
     3841 atctcgatga tgaccggcag atcctcgggc agccgcccgg acaactgccg gtagacgccg
     3901 gcgaacttgt cgagatcggg cttgccggcc tcgctgagcg cggcctcgcc ggagccgaac
     3961 agcacctcgg actggaacac gaagcgatcg ccgacgacgc ggatgtcggg atcgtcctcg
     4021 aacagcgtgc gcagccgccc gaagaactcc gacgcgtagc gcgacaactg ctcgacgcgc
     4081 tcgaccagcc gcagccgaac ctgccggtcg agttcatcga ggcgctcggc ctgttcggtg
     4141 acggtcgcgt cgcgctcggc gagcttcgtc tgcgtcgcgg cgagcgccgt cgcctgttcg
     4201 tccagccgcc cggccagcgc ggcgcgctcg cgttcgagcc gggctttctc ggcctccagg
     4261 gccgcgagct gcgcggcggc ggtctcgcgt tcgaccgccg cgcgctgatg ttcgacgccc
     4321 agcgtcgccg cagcctgcgt gtgcgcctcc gcgagcgtcg ccgccgcctg ccggcgctcg
     4381 gtcgccagcg ccgcggcatg ggcctcggcg tcctgccggc gctgatcctc ggcgagggcc
     4441 agcgcctgct gcaggcgctc gagatcaccc tgcagccgcg acaggcgcag gttcagcccg
     4501 gcaatgccgg cctccgccgc cagccgctgg cgttcgccgg cgtcgagggc ctgtgcctgg
     4561 gcggcggcgc gcgcgagcag cgcatcgcgt tccccgccga gcgcgcgtgc ctgcctgtcc
     4621 gcctcgtcct gcgcctgcag ctgccgggct ttctgctcat cgagcgcgtg gcggctgcgt
     4681 tccagctcgg cgcccagccg caggcgcgtc gcatcggcct gcgcgagcgc ggcgcgcagg
     4741 tcggcaagat caagctcgcg ctcgccgatc gtcgcatcgg cttcggccgc gcgcgtctcg
     4801 gcaacctgtg cccgccgctc gctgagtccg agctgcgtct gcaggcgcgc gatctgcacg
     4861 cgcagctcgc cgatggcgtc atcgcgaccg ctgacctcgc gcgcgagcca ggtctcgccg
     4921 atcacgaaca ccgtgaccag aaacaccagc acggtgacca gccccgacag cgcatcggtg
     4981 aacaccggcc agacgttgta accgccggca gcctcagtgc gacggcggcg catcgtgcgg
     5041 ctccgcggtc tgcgggctga cggcaggcgg ctgcgtgtcc cgggccatgt agcgcgcgat
     5101 cacgcgcagc tcgtgcatca gcgcggcgtt ctgctcgcgg gcctcgcgct ccagccgcac
     5161 cccgagcgcc tgcacgcgct cgtcgccgcg cgcgacggcc tgtgcgagcc gcccggcatc
     5221 gtcgcgtgcc gccgcggctt cgagcaggcc ggcgacgcga tcgagccgct cgatcagcgc
     5281 ctcggcgacc gccagcagat agcgcggcgc caccggctgc tcgcgaccga tcagcgccgg
     5341 cgtctgccag accggcagca gttcggtgac caccagcgtc tccagccggg tcgtgacggc
     5401 gctctgcgcg tggaaaagct gcaattcgag gaagccgagt gcggccgagc agaccagtcc
     5461 gaacagcgag gtcgcaaagc cggtgcccat gccggtcagc ggcgtcgcca gccgcgcgcg
     5521 caggttcgcg agccagtcgg ccccatcgcc cccggccgcc gtgccgagca ggtcgcgcac
     5581 gccctcggtg ctgagcagca aaccgtaaaa ggtgccgatc aggccgatca gcaccagcgt
     5641 gccgcccagg aagcgcacca gcgcgcgcgc atcctccccg cgcccggcga gcgtttcgag
     5701 caggaagggc agcgtctgct gcgcatcggc ctggcgcagc gtcaggcgca cgcgggcgag
     5761 aaattcgccg accgcaccaa ggctcggcag cgtcatcagc tcagccgctt cgccggcagc
     5821 ccgcgtgccg gcgcgcactt cggccgccag cctcgccacg cgcgcgcagg ccgcctgctc
     5881 gcggcgcagc agccccagac agcgcagcgt gtaaccggct ccgaacagga acagcacgag
     5941 gatcaggccg ttcaggtagg ggctgatccg gaaggcgttg gcgagcggcg cgcgcagcag
     6001 cagcgccagc cccgcgagca gcagcagaaa cagtccggcc cgcaccagaa aacggtcgac
     6061 gccggcaccg gccggagcgc tcatcgcagc ggcctcagtc gccgagcggc tgcgcccagc
     6121 cgccctcgaa ggcaattccg gacagcgcca gccgcgtgcg cgccgcggtt tcgcgggtgc
     6181 gcagggcttc gggcagcaca tcggccggtg ccggatgacc gatcgcgatc atcgccatcg
     6241 gcgtcgcgcg ctccggcagg ccggcaagct ttgtgacggc cgcggcatcg aagccgccca
     6301 tctggtgtgc cgccagcccg agcgcagcgg cctggatgca caggttctcg ctggccgcgc
     6361 cggtgtcgta ctgcgcccag cggttcggct ggccgttgtg acggaagtcc gcgcaggcga
     6421 cggcgagcag cagcacggcc gcatgctgcg cccagacgcg gttgccttcg gacagcgtcg
     6481 ccagtgccgc ggcaaagccg gccggatcgc ggctgcggtc ccagcagacg aagcgccagg
     6541 gctgatcccc gaagcaggac ggcgcccagc gcgcggcttc gagcagcgcc tgcagatgcg
     6601 cacgcgccag cggccgtgcg ggatcgagcg cgcgcgggct ccagcgccgg gcgagggccg
     6661 cgtcgatggc aacagtggtc acggccggtt tcccgggcag cggatgaggc atcggttcgg
     6721 acacggcgat ctccggcacg caggga
//
LOCUS       DAOQIE010000221         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6021_length_6729_cov_7.844921, whole genome shotgun sequence.
ACCESSION   DAOQIE010000221 DAOQIE010000000
VERSION     DAOQIE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_6729_cov_7.844921"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..1478
                     /gene="murB"
                     /locus_tag="PK144_13190"
     CDS             444..1478
                     /gene="murB"
                     /locus_tag="PK144_13190"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNF66727.1"
                     /translation="MTAASVLQREVALDGLNTFGLPARAEHFLNLEADSQLAPALAEA
                     ARAQLAIHVLGAGSNVLLAGDVAGLVIRVALCGRRLLLRNGDEVVVEAGAGEDWHGFV
                     AWTLANGWFGLENLALIPGTVGGSPIQNIGAYGVEAGERIAHVDVIDLETGQARRLMP
                     SACGFGYRDSVFKHELAGRVLVVAVAFRLSLQPAVQARYAELNAELQAQGIEAPTPQQ
                     VFEAVCAVRRRKLPEPAVLGNAGSFFKNPVVDFEQATRLRAAHPELVSYPQPDGRVKL
                     AAGWLIERCGWKGRALGRAGVYERQALVLVNHGGATGAEVLALAEAIRADVRTRFGVA
                     LEMEPVRMGG"
     gene            1568..3448
                     /gene="mnmG"
                     /locus_tag="PK144_13195"
     CDS             1568..3448
                     /gene="mnmG"
                     /locus_tag="PK144_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNF66728.1"
                     /translation="MRYPERFDVIVVGGGHAGTEAALAAARCGARTLLLTHNIETVGA
                     MSCNPAIGGIGKGHLVREIDALDGAMARATDRAGIQFRILNARKGPAVRATRAQADRA
                     LYRAAIRSIVENQPNLAVFQQAADDLLLEGTRVSGVRTQTGIEFEAPAVVLTAGTFLA
                     GRIHVGLQNYEGGRMGDPPANTLAARLRELPLAVGRLKTGTPPRIDGRTIDWAQLAAQ
                     PGDDPCPVFSFLGRRDEHPRQIACHITHTNARTHAIIRGGLDRSPMYTGVIEGVGPRY
                     CPSVEDKIVRFADRDAHQIFLEPEGLGTHEVYPNGISTSLPFDVQYALVRSMRGLEHA
                     HLTRPGYAIEYDFFDPRGLHPSLASKHLDGLWLAGQINGTTGYEEAAAQGLLAGLNAA
                     LAVQQREPWWPRRDEAYLGVLVDDLITRGTSEPYRMFTSRAEHRLHLREDNADLRLTE
                     TGRRLGLVGDARWAAFEARREAIERETQRLRECWVRPAALDPALAEAVLGGPLARETR
                     ALDLLRRPELDHAAVLRLAGLPESDLDADVIEQVEIQAGYAGYIERAQADIARARRQE
                     DAAIPAGFDYAGVRGLSSEVREKLARVRPDTVGQAGRIPGVTPAAVSLLLVHLKRLRA
                     AG"
     gene            3635..3913
                     /locus_tag="PK144_13200"
     CDS             3635..3913
                     /locus_tag="PK144_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HNF66729.1"
                     /translation="MKLNVNVEATPQELREFLGLPNVQPLQQEMMEIIRENMLKGVQG
                     FDPVSLMKPFMAGAMPVQSIENLQKVFWDTFNKASTGQPSRTGTTGGS"
     gene            3994..4365
                     /locus_tag="PK144_13205"
     CDS             3994..4365
                     /locus_tag="PK144_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66730.1"
                     /translation="MPKILVVDDEPNILLSLEFLMKHAGFEVRTATDGDAALAAVATE
                     VPDLILLDVMMPRRDGYEVCQAVRENPACARVKIIMLTAKGREIEREKGMALGADDYI
                     VKPFATQELLGRVRELLAGAT"
     gene            4404..6527
                     /locus_tag="PK144_13210"
     CDS             4404..6527
                     /locus_tag="PK144_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNF66731.1"
                     /translation="MTVRIKFWALLLAPLAFTGLFAAVSLWKLPEIVIDPYMQENVRA
                     FYFAGGILACLAYAVVWAFIDAAFVRPLKALLHGARIISRSNPAHELMMPRNHLLGEV
                     PVIVQELGASLHQARKEIAAATATGTREIEAQKSRLEVVLRELSEGVLVCDADARVLL
                     CNPAARRLLPTPEALGLGRSIYGLLTRQPIEHTLDWLGFRQQQAGDGAPPATDAEFLC
                     SAADESRLFQCRMSLLPSSGAARRSFVLTLRDITSQVAPGTLGPSGFKGLVEDLRRQL
                     ASLRFAAESLSLYADMEPGQRRAFERVIFDESAALTERVEFLARHVRLTFAAQWPSAD
                     VLSTDVIGAALSRLERSRGRMPVIVQSGDPLWLHCDGQSVMQLLEFVLTELANQEPAA
                     SFEIECLLGNRQVYVDIVWAGRPLPARLIQDWLERPLQHVAGAATVGEILRRHHSDLW
                     SQPHRKPGLAVLRIPLPSSPRQWSDEDGALPERPEFYDFDLELRPAQFGALADRPLKS
                     LDYVVFDTETTGLSPSRGDEMIQIAGLRVVQGRIREGETFDRLINPGKPIPKASIKFH
                     GITDAMVRDKPGAAVIVPQFRAFVGEPDTVLVAHNAAFDMKFLELKEAVTGVSFRQQP
                     VLDTLLLSGAIHDYTDDHTLDAIAERLGVDVHNRHTALGDTLVTAQVFVKLLDLLEAQ
                     GIVTLGQALALSEQMTAVRRQQAKF"
     gene            6529..6729
                     /locus_tag="PK144_13215"
     CDS             6529..6729
                     /locus_tag="PK144_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66732.1"
                     /translation="MDAGERTARIVALVLLAVLALGWPGLALFDRADSVAGIPLLFLY
                     VYAVWALLIGLLRAWMRGAGED"
BASE COUNT          957 a         2450 c         2388 g          934 t
ORIGIN      
        1 acggcggcga ccagcagccc gccgagggcc gccggcagcg ccgtcgccag actcgatgcg
       61 gcggccgtgt gccggtagac ctgccagccg gcgcgcagcc agcgcagcgg tgcgccggcg
      121 gcgagtcgtg gctgcggcgt cggcacgcgc ggggggaggg cggggtgcgg gagcatcgct
      181 gcgggcctcg ggagacgggg gcggaaatgc cgtcgaggct ggccggtgcg cgggcgcctg
      241 gccttgatcc ggattaaacg cttggtaacc tgcggcttgg gcgcgctgcc ggccggccct
      301 acaatcgccg gcctgcgacc gagggagcct gaccgagccg tgatgccgat cacacctacc
      361 tgcatggttt tctccccctc tccccggtcc ctctcccgca cggggcgagg ggccgaaaag
      421 cgaagcgctg cggcgtgagc ggcatgacgg ccgcatccgt gctgcagcgc gaagtcgcgc
      481 tcgatggcct caacaccttc gggctgccgg cccgggccga gcatttcctg aacctcgaag
      541 ccgacagcca gctcgcgccg gcgctcgccg aggccgcgcg cgcgcagctc gcgatccacg
      601 tgctcggtgc cggcagcaac gtgctgctcg ccggcgacgt ggccgggctg gtgatccgcg
      661 tcgcgctgtg cggccgccgc ctgctgctgc gcaacggcga cgaggtcgtc gtcgaggccg
      721 gcgccggcga ggactggcac ggcttcgtgg cctggacgct cgccaacggc tggttcggcc
      781 tcgaaaacct ggcgctgatc cccggcacgg tcggcggcag cccgatccag aacatcggtg
      841 cctacggcgt cgaggccggc gagcgcatcg cgcacgtcga cgtgatcgat ctcgaaaccg
      901 gccaggcgcg ccggctgatg ccttcggcct gcggcttcgg ctaccgcgac agcgtgttca
      961 agcacgagct ggccggccgg gtgctggtgg tggcggtggc cttccggctg tcgctgcagc
     1021 cggccgtgca ggcgcgctac gccgagctca acgccgagct gcaggcgcag ggcatcgagg
     1081 cgccgacgcc gcagcaggtg ttcgaggccg tctgtgcggt gcgccggcgc aagctgccgg
     1141 agccggccgt gctcggcaac gccggcagct tcttcaagaa cccggtcgtc gatttcgagc
     1201 aggcgacgcg cctgcgcgcc gcgcatccgg agctggtcag ctatccgcag ccggacggcc
     1261 gggtcaagct cgccgccggc tggctgatcg agcgctgcgg ctggaagggc cgcgcgctcg
     1321 ggcgcgccgg cgtctacgag cgtcaggcgc tggtgctggt caaccacggc ggcgccaccg
     1381 gggccgaggt gctggcgctc gccgaggcga tccgcgccga cgtgcgcacg cgcttcggcg
     1441 tcgcactgga gatggagccg gtgcggatgg gcggctgacg acgcggctgc gccccgtgcg
     1501 cgccggcgtg gccggtgcta cgatccgccg ccgttttcct tcctgttttc actggcggat
     1561 caagtccatg cgctatccgg aacgcttcga tgtcatcgtc gtcggcggcg gtcatgccgg
     1621 caccgaggcc gccctggcgg ccgcgcgctg cggcgcgcgc acgctgctgc tgacgcacaa
     1681 catcgagacc gtcggcgcga tgagctgcaa cccggcgatc ggcggcatcg gcaagggaca
     1741 cctcgtgcgc gagatcgacg cgctcgacgg cgcgatggcg cgcgccaccg accgcgccgg
     1801 catccagttc cgcatcctca acgcccgcaa gggcccggcc gtgcgcgcca cgcgcgcgca
     1861 ggccgaccgc gcgctgtatc gcgccgcgat ccgctcgatc gtcgagaacc agccgaacct
     1921 cgccgtgttc cagcaggccg ccgacgacct gctgctcgaa ggcacgcgcg tcagcggcgt
     1981 gcgcacgcag accggcatcg agttcgaggc gccggccgtg gtgctgaccg ccggcacgtt
     2041 cctggccggg cgcatccacg tcggcctcca gaactacgag ggcgggcgca tgggcgaccc
     2101 gccggccaac acgctggcgg cgcgcctgcg cgagctgccg cttgcggtcg ggcggctgaa
     2161 gaccggcacg ccgccgcgca tcgacgggcg cacgatcgac tgggcgcagc tcgccgcgca
     2221 gcccggcgat gatccctgcc cggtgttcag cttcctcggc cgccgcgacg agcacccgcg
     2281 ccagatcgcc tgccacatca cgcacaccaa cgcgcgcacg cacgcgatca tccgcggcgg
     2341 gctcgaccgc tcgccgatgt ataccggcgt catcgagggc gtcggcccgc gctactgccc
     2401 gtcggtcgag gacaagatcg tgcgcttcgc cgaccgggac gcgcaccaga tcttcctgga
     2461 gcccgagggc ctcggcacgc acgaggtcta tccgaacggc atctcgacca gcctgccctt
     2521 cgacgtgcag tacgcgctgg tgcgctcgat gcgcggcctc gaacacgcgc acctgacccg
     2581 gcccggctac gcgatcgaat acgacttctt cgatccgcgc ggcctgcacc cctcgctcgc
     2641 gagcaagcac ctcgacggcc tgtggctggc cgggcagatc aacggcacga ccggctacga
     2701 ggaggctgcc gcgcagggcc tgctcgccgg gctcaatgcc gcgctcgccg tgcagcagcg
     2761 cgaaccctgg tggccgcggc gcgacgaggc ctacctcggc gtgctcgtcg atgacctgat
     2821 cacgcgcggc accagcgagc cgtaccggat gttcacgtcg cgcgccgagc accgcctgca
     2881 cctgcgcgag gacaacgccg acctgcgcct gaccgagacc ggccgccggc tcggactggt
     2941 cggcgatgcg cgctgggcgg cgttcgaggc acggcgcgag gcgatcgagc gcgagacgca
     3001 gcgcctgcgc gagtgctggg tgcgcccggc cgcgctcgac ccggcgctgg ccgaagccgt
     3061 gctcggcggg ccgctcgcgc gcgagacgcg cgcgctcgac ctgctgcgac gcccggaact
     3121 cgatcacgcc gcggtgctgc gcctcgccgg cctgccggaa agcgacctcg atgccgacgt
     3181 gatcgaacag gtcgaaatcc aggcgggcta cgccggctac atcgaacgtg cgcaggccga
     3241 catcgcccgc gcccgccgcc aggaggacgc cgcgatcccg gccggcttcg actacgccgg
     3301 cgtgcgcggg ctgtcgagcg aggtgcgcga gaagctcgcg cgcgtgcgcc ccgacaccgt
     3361 cggtcaggcc gggcgcattc ccggcgtcac gccggcagcg gtgtcgctgc tgctcgtgca
     3421 cctgaagcgc ctgcgcgccg ccggctgagg acggcggcaa gccgcctccg cgcggggcac
     3481 ttgccgctgc ctctcccggt caaattgccg cccgccgcgg ttttcggcta gtgtcgccgg
     3541 ctgccggtgc aagtttgcac cctgcggcag cccgccggac gacgagagca ggcgccgggc
     3601 accggtgaat accaccgacg accgaggaat cacgatgaag ctgaacgtca acgtcgaggc
     3661 cacgccacag gagctgcgcg agttcctggg cctgcccaac gtgcagcccc tgcagcagga
     3721 aatgatggag atcattcgcg agaacatgct gaaaggcgta cagggtttcg atccggtatc
     3781 gctgatgaag ccgttcatgg ccggcgcgat gccggtgcaa tccatcgaaa acctgcagaa
     3841 ggtgttctgg gacaccttca acaaggccag caccggccag ccgtcgcgta ccggtaccac
     3901 cggcggcagc tgagcggggc gggagcgagc cggcatcaga ccggcgcgcg gacgcgggtg
     3961 cgccggggcg ggcgacccgc agagggggga gacatgccga agatactcgt ggtcgatgac
     4021 gagcccaaca tcctgttgtc gctcgaattc ctgatgaagc acgccggttt cgaggtgcgc
     4081 accgccaccg acggcgacgc cgcgctggcc gcggtcgcca ccgaggtgcc cgatctcatc
     4141 ctgctcgacg tgatgatgcc gcggcgcgac ggctacgagg tctgtcaggc cgtgcgtgaa
     4201 aacccggcct gcgcgcgcgt gaagatcatc atgctcaccg ccaagggccg cgagatcgag
     4261 cgcgagaagg gcatggcgct cggggccgac gactacatcg tcaagccctt cgcgacgcag
     4321 gaactgctcg ggcgcgtgcg cgagctgctc gccggggcga cctgaggcga tcccgtcact
     4381 ggccggctca gggggccgcc gtcatgaccg tccgaatcaa gttctgggcg ctgctgctcg
     4441 cgccgctcgc gttcaccggc ctgttcgcgg ccgtgtcgct gtggaagctg cccgagatcg
     4501 tcatcgatcc gtacatgcag gagaacgtcc gcgcgttcta cttcgccggc ggcatccttg
     4561 cgtgtctcgc ctacgccgtg gtctgggcct tcatcgatgc cgccttcgtg cggccgctga
     4621 aggcgctgct gcacggggcg cgcatcatca gccgcagcaa cccggcgcac gaactgatga
     4681 tgccgcgcaa ccacctgctc ggcgaggtgc cggtgatcgt gcaggagctc ggcgccagcc
     4741 tgcatcaggc gcgcaaggag atcgccgccg ccaccgccac cggcacgcgc gagatcgagg
     4801 cgcagaagtc gcggctcgaa gtcgtgctgc gcgagctgag cgaaggcgtg ctggtctgcg
     4861 acgccgatgc gcgcgtgctg ctctgcaacc cggccgcgcg ccggctgctg ccgacgcccg
     4921 aggcgctcgg gctcggccgt tcgatctacg gcctgctgac ccgccagccg atcgagcaca
     4981 cgctcgactg gctgggcttc cgccagcagc aggccggcga cggtgcgccg ccggcgaccg
     5041 atgccgagtt cctgtgctcg gcggccgacg aatcgcggct gttccagtgc cgcatgagcc
     5101 tgctgccgag cagcggcgcg gcgcggcgca gcttcgtgct gacgctgcgc gacatcacca
     5161 gccaggtcgc accgggcacg ctcggcccga gcggcttcaa gggtctggtc gaggacctgc
     5221 gccgccagct cgccagcctg cgcttcgcgg ccgagtcgct gtcgctgtac gcggacatgg
     5281 agccgggcca gcgccgcgcc ttcgagcgcg tgatcttcga cgagagcgcg gcgctgaccg
     5341 agcgcgtcga attcctcgcc cggcacgtgc gcctgacctt cgccgcgcag tggccgagcg
     5401 ccgacgtgct cagcaccgac gtgatcggcg cggcgctgtc ccgcctcgaa cgcagccgcg
     5461 ggcgcatgcc ggtcatcgtc cagtccggcg acccgctgtg gctgcactgc gacggccaga
     5521 gcgtcatgca gctccttgaa ttcgtgctga ccgagcttgc caaccaggag ccggcggcga
     5581 gcttcgagat cgaatgcctg ctcggcaacc ggcaggtcta cgtcgacatc gtctgggccg
     5641 gccggccgct gccggcgcgg ctgatccagg actggctgga gcggccgctg cagcacgtcg
     5701 ccggcgccgc gaccgtcggc gagatcctgc gccgccacca ctccgacctc tggagccagc
     5761 cgcaccgcaa gccgggcctc gcggtgctgc gcatcccgct gccgtcctcg ccgcggcagt
     5821 ggagcgacga ggacggcgcg ctgcccgagc gcccggagtt ctacgacttc gacctcgaac
     5881 tgcgcccggc gcagttcggc gcgctcgccg accggccgct gaagtcgctc gactacgtgg
     5941 tcttcgatac cgagaccacc gggctctcgc cgtcgcgcgg cgacgagatg atccagatcg
     6001 ccggcctgcg cgtcgtgcag ggccgcatcc gcgagggcga gaccttcgac cggctgatca
     6061 accccggcaa gccgatcccg aaggcctcga tcaagttcca cggcatcacc gatgccatgg
     6121 tccgcgacaa gcccggcgcc gccgtgatcg tgccgcagtt ccgcgccttc gtcggcgagc
     6181 cggacacggt actggtcgca cacaacgccg ccttcgacat gaagttcctc gaactgaagg
     6241 aagccgtgac cggcgtgagc ttccgccagc agccggtgct cgacacgctg ctgctctccg
     6301 gcgcgatcca cgactacacc gacgaccaca cgctcgacgc catcgccgag cgcctcggcg
     6361 tcgacgtgca caaccgccac accgcgctcg gcgacacgct ggtcaccgcg caggtgttcg
     6421 tgaagctgct cgacctgctc gaagcccagg gcatcgtcac gctcgggcag gcgctggcgc
     6481 tgtccgaaca gatgacggcc gtgcgccgcc agcaggcgaa gttctgacat ggacgccggc
     6541 gagcgcacgg cccgcatcgt cgcgctggtg ctgctggcgg tgctcgcact cggctggccg
     6601 gggctcgcgc tgttcgaccg cgccgacagc gtcgccggca tcccgctgct gtttttgtac
     6661 gtctacgcgg tctgggcgct gctgatcggg ctgctgcggg cgtggatgcg cggggcgggc
     6721 gaggactga
//
LOCUS       DAOQIE010000222         6714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6034_length_6714_cov_7.807479, whole genome shotgun sequence.
ACCESSION   DAOQIE010000222 DAOQIE010000000
VERSION     DAOQIE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_6714_cov_7.807479"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PK144_13220"
     CDS             <1..448
                     /locus_tag="PK144_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNF66733.1"
                     /translation="NYVRRNFGVLIGEPTAERIKQEIGSAYPTSDVREIEVKGRNLSE
                     GVPRSFILNSNEILEALQEPLSGITGAVKKALESTPPELAADIAERGIVLTGGGALLR
                     DIDRLLMEETGLNVVVAEDPLTCVARGGGRVLELIDERGAQTFAVD"
     gene            513..1472
                     /gene="mreC"
                     /locus_tag="PK144_13225"
     CDS             513..1472
                     /gene="mreC"
                     /locus_tag="PK144_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNF66734.1"
                     /translation="MGKLESRQSKSLFAPETAASIRYALLALLSVVLMTVDHRQHHLE
                     LMRNVLATALYPLQVTVQLPFEAADWLAQHFESRSALVEENERLREKQLFINAQLQKL
                     TALEAENRRLRSLLESTVNVAAERVLIAEILMVDFDPYRHQVLLNRGTQQGARIGQPL
                     IDERGIVGQVAHANLLDSTAILITDPAHALPVQLTRNGIRSLAVGTGRFSELELPNIP
                     NNGDVKVGDLVVTSGLGGRFPRGYPVARVTRVEFDPGRPFARVFAQPTAHLDRVREVL
                     MILDDIADPAAHPGAAAVAPVPDAAKPDRHPKPDKPAAAAGRH"
     gene            1477..1968
                     /gene="mreD"
                     /locus_tag="PK144_13230"
     CDS             1477..1968
                     /gene="mreD"
                     /locus_tag="PK144_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNF66735.1"
                     /translation="MGSIETSRGYGLVLLTFVVALMLSSLHLPPVIDRFRPDWVGLVL
                     TYWALALPNRVGVGTGWVLGLIQDTAQGTLLGQHALGLATVAWLTVRFHQRIRVFPLW
                     QQALSVASFLMIEQLLVVWIKGITGYPPGDWWYLAPPLGGMLAWPWVFIVLRDLRRRF
                     QVS"
     gene            1989..3884
                     /gene="mrdA"
                     /locus_tag="PK144_13235"
     CDS             1989..3884
                     /gene="mrdA"
                     /locus_tag="PK144_13235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNF66736.1"
                     /translation="MSRPLRDTRAARKLFAGRALVAGVLVFLLFSVIAARLAWLQIVN
                     HEHFATLSDQNRIRLVPVPPTRGQIFDRKGVLLAGNLASFHLEITPEQVGSHAQMEAL
                     LEQLKTLIELSDSEINRFRRLLRRSPRYNGVPLRFNLTEDEMARVAINQFRLPGVDIR
                     NDLTRTYPLGTHMAHVVGYVGRIDEQELARLDPAEYSGSSHIGKIGVEKSYEDVLRGK
                     VGYQQVETNAQGRVIRVLESTPPTAGRNVYLTIDSRVQRVAELALSEANFNGAIVAID
                     PRNGELIAMASNPSYDPNPFVNGIDAKSYKALNTSPDRPLYNRALRGMYPPGSTIKPF
                     MGLAGLAYNAVDRYSSIFCGGYFQLPGQDHKFRDWRRGGHGRTDLDRAITESCDVYFY
                     DLALELGIDRIGAFLGQFGFGKPTGIDLRNEKSGLLPSQDWKRRTYNQAWFPGDTVSV
                     GIGQGYLLVTPLQLASATATLAMGGRRFQPRIVHALEDPALRRITELPAVALPSVAAP
                     DRRIFEWVTAFMTHVVHSERGTAFRAVGKFSAYRIAGKTGTAQVFTVGQNERYDASRL
                     KDELLDHALFMAFAPADAPRIAVAVIAENGGHGGSVAAPLAKKVMDAYLLPTNAAGAP
                     AGASPPP"
     gene            3892..5031
                     /gene="rodA"
                     /locus_tag="PK144_13240"
     CDS             3892..5031
                     /gene="rodA"
                     /locus_tag="PK144_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNF66737.1"
                     /translation="MSPYAFQDAPLHDREAPRPRLLARVHLDAPLLLGLLAVAAIGLV
                     VLYSASLQDLESVIRQGTRLGLGFVVLIVLAQVPPTLLRTWTPALYAIGNLLLVAVLL
                     VGDHSMGAQRWLDLGFVRFQPSEIMKIAVPLMLAWHFADKQLPPRVPHLLVGAVIAGV
                     PFVLIAKQPDLGTGLVVFSAGLFALFLGGMSWKIIFGLIAAIAAVAPVFWEYGMREYQ
                     RQRVRTLFDPEADPLGAGYHIIQSKIAIGSGGLYGKGWLNGTQSHLEFLPESHTDFIL
                     GTFAEEFGLIGVCILLVLYFLIVARALRLAAGAGDTYGRIAGGTLALVFFFYAFVNTG
                     MVSGILPVVGVPLPLVSYGGTSLVTIMAGFGILMSLHFHRRRVGH"
     gene            5063..6112
                     /gene="mltB"
                     /locus_tag="PK144_13245"
     CDS             5063..6112
                     /gene="mltB"
                     /locus_tag="PK144_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNF66738.1"
                     /translation="MIRLLSVAAAGLLLGACATATPPVVEARPRPVAAAEPATGQPFA
                     GRSEVDAFIREMTARHGIDAGALRRAFAQSASRPEIIELMDRPAEAKPWSAYRKIFLT
                     EKRIGGGVEFMRANAAALARAEARYGVPGQVITAIIGVETLYGANTGKFPVFEALATL
                     AFDYPRRADYFRKELENFLVLATEEGLDFRQPLGSYAGAMGLAQFMPSSYRRLAIDFD
                     GDGHRNLWTDPEDAIGSIANYLANSGWRRADPLIALPARLSGPGWEGAVSSNFRAPKS
                     LTRLSIAQWRARGVEPAAAVDAARTALLVTLDGETGLEPYLCFDNFYAITRYNLSALY
                     AMAVTQLSEAIAARR"
     gene            6133..>6714
                     /locus_tag="PK144_13250"
     CDS             6133..>6714
                     /locus_tag="PK144_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF66739.1"
                     /translation="MPANAKMRVTVRLLLGAGALWLAACSTPDSGDRRAEPPSRSGNP
                     ESYVVFGKRYQVLGTSEGYRERGIASWYGDAFHGKKTSSGVPFDMHEISAAHKTLPIP
                     TWVKVTNLRNGKSLVLRVNDRGPFVGDRVIDLSFAAARALDVTGRGTAEVELEAVAPF
                     QTLADAHRRPKPGDAGTLLASRDRGSADVLAAAA"
BASE COUNT          968 a         2463 c         2241 g         1042 t
ORIGIN      
        1 caactacgtg cgccgcaact tcggcgtgct gatcggcgaa ccgaccgccg agcgcatcaa
       61 gcaggagatc ggcagcgcct acccgacctc ggacgtgcgc gagatcgagg tcaagggccg
      121 caacctctcc gagggcgtgc cgcgcagctt catcctgaac tccaacgaga tcctcgaagc
      181 cctgcaggaa ccgctgtccg gcatcaccgg cgcggtcaag aaggcgctgg aatcgacgcc
      241 gccggagctg gccgccgaca tcgccgagcg cggcatcgtg ctgaccggcg gcggggcgct
      301 gctgcgcgac atcgaccggc tgctgatgga ggagaccggc ctcaacgtcg tcgtcgccga
      361 ggacccgctg acctgcgtcg cccgtggcgg cggccgggtg ctcgaactga tcgacgagcg
      421 cggcgcgcag actttcgcgg tcgactgacc gcctcccctg cggccgtctg catccgcggc
      481 cgcgggcctg ctcccgtcgc cggggggacg ccgtgggcaa gctggaatcc cgccagtcga
      541 aatcgctgtt cgcaccggaa acggccgcgt cgatccgcta tgcgctgctc gcgctgctgt
      601 cggtggtgct gatgacggtc gaccaccgcc agcaccacct ggagctgatg cgcaacgtgc
      661 tggccaccgc gctgtacccg ctgcaggtca ccgtgcagct gccgttcgag gccgccgact
      721 ggctggcgca gcacttcgaa tcgcgctcgg cgctggtcga ggagaacgag cgcctgcgcg
      781 agaagcagct gttcatcaac gcccagctgc agaagctcac cgcgctcgag gccgagaacc
      841 gccggctgcg ctcgctgctg gaatcgacgg tcaacgtcgc ggccgagcgc gtgctgatcg
      901 ccgagatcct gatggtcgac ttcgacccct accgccacca ggtgctgctc aaccgcggca
      961 cgcagcaggg cgcgcgcatc ggccagccgc tgatcgacga gcgcggcatc gtcggccagg
     1021 tcgcgcacgc caacctgctc gattcgacgg cgatcctgat caccgatccc gcgcacgcgc
     1081 tgccggtgca gctgacgcgc aacggcatcc gctcgctcgc ggtcggcacc gggcgcttca
     1141 gcgagctgga gctgccgaac atcccgaaca acggcgacgt caaggtcggc gatctggtcg
     1201 tgacctccgg cctcggcggg cgctttccgc gcggctatcc ggtcgcgcgc gtcacgcgcg
     1261 tcgagttcga ccccggccgg ccgttcgcgc gcgtgttcgc gcagccgacc gcgcatctcg
     1321 atcgcgtgcg cgaggtgctg atgatcctcg acgacatcgc cgatccggcc gcccaccccg
     1381 gagccgccgc cgtcgcgccc gtgccggatg ccgcgaagcc ggatcgccat ccgaagcccg
     1441 acaagccggc cgcggccgca ggcaggcact gaaaccatgg ggtcgatcga aacctcgcgc
     1501 ggctacgggc tggtgctgct gaccttcgtc gtcgcgctga tgctctccag cctgcacctg
     1561 ccgccggtca tcgaccgctt ccggcccgac tgggtcgggc tggtgctgac ctactgggcg
     1621 ctggcgctgc cgaaccgcgt cggcgtcggc accggctggg tgctcgggct gatccaggac
     1681 accgcccagg gcacgctgct cggccagcac gcgctcgggc tggcgacggt cgcctggctg
     1741 accgtgcgtt tccaccagcg catccgcgtg ttcccgctat ggcagcaggc gctgtcggtg
     1801 gcctcgttcc tgatgatcga acagctgctg gtggtctgga tcaagggcat caccggctat
     1861 ccgcccggcg actggtggta tctggccccg ccgctcggcg gcatgctggc ctggccgtgg
     1921 gtgttcatcg tgctgcgcga cctgcgccgg cgcttccagg tttcctgagc gtttccggat
     1981 cgagtgccat gagtcgcccg cttcgcgaca cgcgcgccgc gcgcaagctg ttcgccggcc
     2041 gcgccctggt ggccggcgtg ctggtgttcc tgctgttctc ggtgatcgcc gcgcgtctgg
     2101 cctggctgca gatcgtcaac cacgagcact tcgcgacgct gtcggaccag aaccgcatcc
     2161 ggctggtgcc ggtgccgccg acgcgcggcc agatcttcga ccgcaagggc gtgctgctcg
     2221 ccggcaacct ggcgtcgttt catctggaga tcacgcccga gcaggtcggc agccacgcgc
     2281 agatggaggc gctgctcgaa cagctcaaga cgctgatcga gctgtcggac tccgagatca
     2341 accgcttccg gcgcctgctg cgccgcagcc cgcgctacaa cggcgtgccg ctgcgcttca
     2401 acctcaccga ggatgaaatg gcgcgcgtgg cgatcaacca gttccggctg cccggcgtcg
     2461 acatcaggaa cgacctgacc cgcacctatc cgctcgggac gcacatggcg cacgtcgtcg
     2521 gctacgtcgg acgcatcgac gagcaggagc ttgcgcgcct cgatccggcc gagtactcgg
     2581 ggtcgagcca catcggcaag atcggcgtcg agaagtccta cgaggacgtg ctgcgcggca
     2641 aggtcggcta ccagcaggtg gagaccaacg cccagggccg cgtgatccgc gtgctggaga
     2701 gcacgccgcc gaccgccggg cgcaacgtct acctgaccat cgactcgcgc gtgcagcgcg
     2761 tcgccgaact cgcactcagc gaggcgaact tcaacggtgc catcgtcgcg atcgacccgc
     2821 gcaacggcga gctgatcgcg atggcctcga acccgtccta cgatccgaat ccgttcgtca
     2881 acggcatcga cgccaagagc tacaaggcgc tgaacacctc gccggaccgg ccgctgtaca
     2941 accgcgcgct gcgcggcatg tacccgcccg gctcgacgat caagccgttc atgggtctgg
     3001 ccggcctcgc ctacaacgcc gtcgaccgct actcgtcgat cttctgcggc ggctacttcc
     3061 agctgccggg gcaggatcac aagttccgcg actggcgccg cggcggccac ggccgcaccg
     3121 acctcgaccg cgcgatcacc gagtcctgcg acgtgtactt ctatgacctc gcgctcgaac
     3181 tcggcatcga ccgcatcggg gccttcctcg gccagttcgg cttcggcaag ccgaccggca
     3241 tcgacctgcg caacgagaag agcggcctgc tgccctcgca ggactggaag cggcgcacct
     3301 acaaccaggc ctggttcccc ggcgacacgg tcagcgtcgg catcggccag ggctatctgc
     3361 tggtgacgcc gctgcagctc gcgagcgcga cggcgacgct ggcgatgggc gggcggcgct
     3421 tccagccgcg catcgtgcat gcgctcgagg acccggcgct gcggcgcatc accgaactgc
     3481 cggcggtggc gctgccaagc gtcgccgcgc cggaccggcg catcttcgaa tgggtcacgg
     3541 ccttcatgac ccacgtcgtg cactccgaac gcggcacggc gttccgggcg gtcggcaagt
     3601 tctcggccta ccgcatcgcc ggcaagaccg gcaccgcgca ggtgttcacg gtcggccaga
     3661 acgagcgcta cgacgccagc cgcctgaagg atgaactgct cgatcacgcg ctgttcatgg
     3721 ccttcgcacc ggccgatgcg ccgcgcatcg ccgttgccgt gatcgccgag aacggcggcc
     3781 acggcggttc ggtggctgcg ccgctggcca agaaggtcat ggatgcctac ctgctgccga
     3841 ccaacgccgc gggcgccccc gccggtgcga gcccgccgcc atgaggtcgt catgagtccg
     3901 tacgccttcc aggatgcccc gctgcacgat cgcgaggcac cgcgcccgcg gctgctggcg
     3961 cgcgtgcacc tcgatgcgcc gctgctgctc ggcctgctcg cggtcgccgc gatcgggctg
     4021 gtcgtgctct acagcgcctc gctgcaggat ctggagtcgg tgatccgcca gggcacgcgc
     4081 ctcgggctcg gcttcgtcgt gctgatcgtg ctcgcgcagg tgccgccgac gctgctgcgg
     4141 acatggacgc cggcgctgta tgcgatcggc aacctgctgc tggttgcggt cctgctggtc
     4201 ggcgatcact cgatgggcgc gcagcgctgg ctcgatctcg gcttcgtgcg cttccagccc
     4261 tcggaaatca tgaagatcgc cgtgccgctg atgctcgcgt ggcacttcgc cgacaagcag
     4321 ttgccgccgc gcgtgccaca cctgctggtc ggtgcggtga tcgccggcgt gcccttcgtg
     4381 ctgatcgcca agcagccgga cctcggcacc ggcctcgtgg tgttctcggc cgggctgttc
     4441 gcgctgtttc tcggcggcat gtcgtggaag atcatcttcg gcctgatcgc cgcgatcgct
     4501 gcggtcgcgc cggtgttctg ggaatacggc atgcgcgaat accagcgcca gcgcgtgcgc
     4561 acgctgttcg acccggaggc cgatccgctc ggtgccggct accacatcat ccagtcgaag
     4621 atcgcgatcg gctccggcgg cctgtacggc aagggctggc tgaacggcac gcagtcgcac
     4681 ctcgaattcc tgcccgagtc gcacaccgac ttcatcctcg gcaccttcgc cgaggagttc
     4741 gggctgatcg gcgtctgcat cctgctggtg ctgtacttcc tgatcgtcgc gcgcgcgctg
     4801 cgcctcgcgg ccggggccgg cgacacctac gggcgcatcg ccggcggcac gctggcgctg
     4861 gtgttcttct tctatgcctt cgtcaacacc ggcatggtca gcggcatcct gccggtggtc
     4921 ggcgtgccgc tgccgctggt cagctacggc ggcacctcgc tcgtgacgat catggccggc
     4981 ttcggtatcc tcatgagcct ccacttccac cgccgccgcg tcgggcactg agcgcgacgc
     5041 gccggaaggg cttccctgcc gcatgatccg cctcctgtcc gtggccgccg ccggcctgct
     5101 gctcggcgcc tgtgccaccg ccacaccgcc cgtcgtcgaa gcccgcccgc gcccggtcgc
     5161 cgctgccgaa ccggccaccg gccagccctt cgccggtcgc agcgaagtcg acgccttcat
     5221 ccgcgagatg accgcaagac acggcatcga cgccggggcg ctgcgccgcg ccttcgcgca
     5281 gagcgcctcg cgccccgaga tcatcgaact gatggaccgc ccggccgaag ccaagccgtg
     5341 gtcggcctac cggaaaatct tcctgaccga aaagcgcatc ggcggcggcg tcgagttcat
     5401 gcgcgccaac gccgcggcgc tggcgcgcgc cgaggcccgc tacggcgtgc ccgggcaggt
     5461 gatcaccgcg atcatcggcg tcgagaccct ctacggcgcc aacaccggca agttcccggt
     5521 gttcgaggcg ctggcgacgc tggccttcga ctacccgcgc cgtgccgact acttccgcaa
     5581 ggaactggag aacttcctgg tgctcgcgac cgaggaaggt ctcgacttcc gccagccgct
     5641 cggctcctac gccggcgcga tggggctcgc gcagttcatg ccgtcgagct accggcgcct
     5701 cgcgatcgac ttcgacggcg acggccaccg caacctctgg accgaccccg aggatgcgat
     5761 cggcagcatc gccaactacc tcgccaacag cggctggcgc cgcgccgatc cgctgatcgc
     5821 gctgccggca cgcctgagcg gcccgggctg ggaaggtgcg gtgtcgagca acttccgcgc
     5881 gccgaagtcg ctgacgcgcc tgagcatcgc gcagtggcgc gcgcgcggcg tcgagccggc
     5941 cgcggccgtc gatgcggcac gcacggcgct gctggtgacg ctcgacggcg agaccggact
     6001 ggagccgtat ctctgcttcg acaacttcta cgcgatcacg cgctacaacc tcagcgccct
     6061 gtacgcgatg gcagtcacgc agctgtccga agcgatcgcg gcccggcgct gagccagcgg
     6121 agacgagggc cgatgcctgc gaacgcgaag atgcgagtca ccgtgcgact gctgctgggg
     6181 gccggcgcgc tgtggctggc ggcgtgctcg acgcccgatt cgggcgaccg gcgtgccgaa
     6241 ccgccgagcc gctccggcaa ccccgagtcc tacgtggtgt tcggcaagcg ctatcaggtg
     6301 ctcggcacca gcgagggtta ccgcgagcgc ggcatcgcct cgtggtatgg cgatgccttc
     6361 cacggcaaga agacctccag cggcgtgccc ttcgacatgc acgagatctc ggccgcgcac
     6421 aagacgctgc cgatcccgac ctgggtgaag gtcacgaacc tgcgcaacgg caagtcgctg
     6481 gtgctgcgcg tcaacgaccg cggccccttc gtcggcgatc gcgtcatcga cctgtcgttc
     6541 gccgccgcca gggcgctcga tgtcaccggc cgcggcacgg ccgaggtcga actcgaagcc
     6601 gtcgcgccct tccagaccct cgccgatgcc caccgccggc cgaagcccgg cgatgctggc
     6661 acgctgctcg cgtcgcgcga tcgcggcagt gccgacgtgc tcgccgccgc cgcc
//
LOCUS       DAOQIE010000223         6688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6083_length_6688_cov_6.876828, whole genome shotgun sequence.
ACCESSION   DAOQIE010000223 DAOQIE010000000
VERSION     DAOQIE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_6688_cov_6.876828"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1098)
                     /locus_tag="PK144_13255"
     CDS             complement(100..1098)
                     /locus_tag="PK144_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF66740.1"
                     /translation="MSSSRSDRSASPAAPSIASGQPPSAGLTRRQALGCLFWAGTGAL
                     WAMHGGVPRWLGAIGDANAAEAKTDAGAFRFVQISDSHIGFNKAANPDPLLTLNLALD
                     KVRALPERPALMLHTGDVTHLSKPAEFDTAAQLIGGLGLPTFYVPGEHDVIDEGAGRA
                     WLDRYGKGTQGAGWYSFEDHGVHFVGLVNVLDFKPGGLGRLGAAQLAWLADDLKAVRD
                     STPVVVFAHIPLWTVYADWGWGTDDAAQALALLKRFGSVTVLNGHIHQTLQKVEGNVS
                     FHTAYSTAYPQPAPGTAASPGPLVVPAEQLRHVLGVRSLQLTRTAPGAQLTDYPLA"
     gene            complement(1169..1885)
                     /locus_tag="PK144_13260"
     CDS             complement(1169..1885)
                     /locus_tag="PK144_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNF66741.1"
                     /translation="MTLPAAERTAGATLHEQVYQSLRGAIMCGRFVPGRALTIRGVAA
                     MLGTSLMPVREALRRLVAERALELLANRRVSVPHMTPARLDELCAARLALEPLAAVRA
                     LPHVDGTRLAELRQIDAGIDAALAAGEVERYLQLNQLFHLTLYRAGEPQVLIPLIESL
                     WLQFGPFMRATIGRIGASYVLDRHVEALAALERRDPLALRLAIESDIRDGMGSLSHEE
                     LLRLYRDAAAPAERSASARE"
     gene            2009..3385
                     /locus_tag="PK144_13265"
     CDS             2009..3385
                     /locus_tag="PK144_13265"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNF66742.1"
                     /translation="MSPANVAHDEVGRFLAAHPEVEVFELLIPDTNGVLRGKWVPRSA
                     VEKVYSAGIRLPSSVFALDIWGNDVLASGLVIETGDRDGLCKPVAGSLTLVPWLKRPT
                     AQVLMAMYDPDSVQPFFGDPRQVLAQVLAGYTALGLTPVVALELEFYLLDREPSPDGS
                     PQPPLSPGTGRRQWSSQVYGIDELHEFEDFFDGVARACVLQRLPVDTTVAEQAPNQYE
                     INLKHVDDAILAADNAVLLKRVIKGVAQQHQMAATFMAKPYGELAGNGMHIHFSLIDE
                     RGFNVFDDGSAEGSALLKHAIAGMVTTMRETMALYAPNTNSYRRFQPGSHAPVSANWG
                     YDNRAAALRIPSGERQAMRIEHRLAGADANPYLALAAMLAGGLRGIREKLDPGEPSRG
                     HVGNGQAPVLPASLKHALEIFDKSPFPREAFGDSYPRLYSACRWQELEKFGKQVTPLE
                     YDSYLRTV"
     gene            3447..3902
                     /locus_tag="PK144_13270"
     CDS             3447..3902
                     /locus_tag="PK144_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66743.1"
                     /translation="MTRLSSEDAEMAHEKKIAHLTFIQGIITRMGNNSFLLKGWTVTL
                     VAALFALAAKDSNQNFILIAYFPILVFWMLDGYFLYQERLFRDLYNGVANDRISSEQF
                     EINPASLMSDSGKWQPAFLWARAALSKTLLVFYMAMMGVTLFVMCFLLR"
     gene            4268..4795
                     /locus_tag="PK144_13275"
     CDS             4268..4795
                     /locus_tag="PK144_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005738168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNF66744.1"
                     /translation="MAVRDALAIDLLAICEIYTPYVLYGAQTFEEVPPSLAAMTERRA
                     QVLAHGLPFLVAERGARVLGYCYATPYRSRPAYRYTVEDSIYIAHDCTAGGVGTALLE
                     ALIRRCERGPWRQMISVIGDSDNAASIRLHTRHGFEPIGICRAVGFKHGRWIDTVLMQ
                     RALGAGDRSLPSGRA"
     gene            4890..6152
                     /locus_tag="PK144_13280"
     CDS             4890..6152
                     /locus_tag="PK144_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66745.1"
                     /translation="MLFSPVPPTAAEAARLHRYRLLLIALVAGAHALADAVLGLDLPW
                     AMLALVLGGWLLLGQAARALRLATLLQFGLDLVLLTVVFATTGGFGNPLVSLYLLPLA
                     LGATLLRRRLAWMLTGLAIGMATLLIVVWTPAAVGLHTAHAAQHPHALSGGLGYASHL
                     VGMWLSFVAAAGLLVHFTGAMAVVLRRRERELAAAREQTLRDRQVLALGALAAGAAHE
                     LGTPLATIAVLARELELDHAGDAALAADLATLRTQVEHCKHAISSLLAAAGQARVEAA
                     GTQALDVWLETLLARWQTLRPAVPVQLQLAAGASPSILTDDTLAQSLVSLLNNAADVS
                     PAGIGLRADWEGGGLRLAVLDDGPGLPARAGEPHWTGKLHGRGLGIFLARAAIERLGG
                     ELRYTLRPEGGTCAEIRLPNAALAVNPT"
     gene            6149..>6688
                     /locus_tag="PK144_13285"
     CDS             6149..>6688
                     /locus_tag="PK144_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF66746.1"
                     /translation="MSDAAADRPTLLLADDDDTFRSVLARALTRRGYAVRPAADVAEA
                     RRLAAIEPPEFAVVDLKMPGESGLALVEALVALDAGTRIIVLTGYASITTAVEAIKLG
                     AVHYLAKPADADQIVAAFGRLDGNARVPVSDTPMSVDRLEWEHIQRVLAEHGGNVSAT
                     ARALHMHRRTLQRKLGKRPV"
BASE COUNT          984 a         2335 c         2329 g         1040 t
ORIGIN      
        1 acatggcagg gattccggag gtcgggcggc gatggacgcg gggccgggcg gctcgaaagc
       61 gccggtgccc ggcgtcgggg cagaaggcgc gggccggcgt caggccagcg ggtagtcggt
      121 cagctgcgca ccgggtgcgg tgcgggtcag ctgcaggctg cgcacgccga gcacgtgccg
      181 gagctgttcg gccggcacca ccagcgggcc gggcgaggcc gcggtgccgg gggccggctg
      241 cgggtaggcg gtcgagtagg cggtgtggaa gctgacgttg ccctcgacct tctgcagcgt
      301 ctggtggatg tggccgttca gcaccgtcac cgagccgaag cgcttcagca gcgcgagcgc
      361 ctgcgcggcg tcgtcggtgc cccagcccca gtcggcgtac acggtccaca gcgggatgtg
      421 tgcgaacacc acgaccggcg tcgaatcgcg cacggccttc aggtcatcgg cgagccacgc
      481 aagctgcgcg gccccgagcc ggccgaggcc gccgggcttg aagtcgagca cgttgaccag
      541 gccgacgaaa tgcacgccgt ggtcctcgaa gctgtaccag ccggcgccct gcgtgccctt
      601 gccgtagcga tcgagccagg cccgcccggc gccttcgtcg atcacgtcgt gctcgcccgg
      661 cacgtagaag gtcggcagcc cgagtccgcc gatcaactgc gcggcggtgt cgaactcggc
      721 cggcttcgac aggtgcgtga catcgccggt gtgcagcatc agcgccggac gctcgggcag
      781 cgcgcgcacc ttgtcgagcg cgaggttcag cgtcagcagc ggatcggggt tcgcggcctt
      841 gttgaagccg atgtggctgt cgctgatctg cacgaagcgg aaggcaccgg catcggtctt
      901 ggcctcggcc gcattcgcgt caccgatcgc accgagccag cgcggcacgc cgccgtgcat
      961 tgcccacagc gcgccggtgc cggcccagaa caggcagccg agcgcctgcc ggcgcgtgag
     1021 gccggcggat ggcggctgtc cggatgcgat ggagggggcg gcgggtgaag cgctgcggtc
     1081 ggaacgggat gaggacatgg gggcggtctc cgctgctgcg gccccggaag cgggaccgtt
     1141 gcacgggaga cgcgccgggc cgggtggttt attcccgggc ggatgcagat cgctctgccg
     1201 gtgccgcagc gtcccggtac agccgcagca gttcctcgtg gctcagcgag cccatgccgt
     1261 cgcggatgtc gctctcgatc gccaggcgca gcgccagcgg atcgcgccgt tcgagcgcgg
     1321 ccagcgcctc gacgtggcga tcgagcacgt agctcgcgcc gatgcggccg atggtcgcgc
     1381 gcatgaacgg cccgaactgc agccacaggc tctcgatcag cgggatcagc acctgcggct
     1441 cgccggcgcg gtacagcgtc aggtgaaaga gctggttgag ctgcaggtaa cgctcgacct
     1501 cgccggcagc gagcgcggcg tcgatgccgg cgtcgatctg ccggagttcc gcgagccgcg
     1561 tgccgtcgac gtgcggcagc gcccgcaccg ccgccagcgg ctccagcgcg aggcgcgcgg
     1621 cgcagagctc atcgagccgc gccggcgtca tgtgcggcac gctgacgcgg cggttggcga
     1681 gcagttccag cgcgcgctcg gcgaccagcc ggcgcagcgc ctcgcgcacc ggcatcaggc
     1741 tggtgccgag catcgccgcg acgccgcgga tggtcagcgc ccgccccggc acgaagcgcc
     1801 cgcacatgat cgcgccgcgc agcgactggt agacctgttc gtgcagggtg gcgccggcgg
     1861 tgcgctcggc ggcgggcagc gtcacgggca tcggggtggg aggcggtggg ctcgacagct
     1921 ccggaaacat gtgatcacaa tcggccggca gtcttcgcga acacccgaac ggcgtcaagc
     1981 gatgcccctg cccccgagga atcctccgat gagtcccgcc aacgtcgcgc acgacgaggt
     2041 cggccgcttc ctggccgcgc atcccgaagt cgaagtcttc gagctgctga tcccggacac
     2101 caacggcgtg ctgcgcggca agtgggtgcc gcgcagtgcg gtcgagaagg tctacagcgc
     2161 cggcatccgc ctgccttcgt cggtgttcgc gctcgacatc tggggcaacg acgtgctcgc
     2221 cagcggcctc gtgatcgaga ccggcgaccg cgacggcctc tgcaagccgg tcgccggttc
     2281 gctgacgctg gtgccgtggc tcaagcgccc gaccgcgcag gtgctgatgg cgatgtacga
     2341 cccggacagc gtccagccct tcttcggcga tccgcgccag gtgctcgcgc aggtgctggc
     2401 cggctacacg gcgctcgggc tgacgccggt cgtggcgctg gagctggagt tctacctgct
     2461 cgatcgcgag ccctcgccgg atggctcgcc gcagccgccg ctgtcgcccg gcaccggacg
     2521 ccggcagtgg agttcgcagg tctacggcat cgacgagctg cacgagttcg aggacttctt
     2581 cgacggcgtc gcccgcgcct gcgtgctgca gcgcctgccg gtcgacacca cggtcgccga
     2641 acaggcgccg aaccagtacg agatcaacct caagcacgtc gacgatgcca tcctcgccgc
     2701 cgacaacgcc gtgctgctca agcgcgtgat caagggcgtc gcgcagcagc accagatggc
     2761 cgcgaccttc atggccaagc cctatggcga actcgccggc aatggcatgc acatccactt
     2821 cagcctgatc gacgagcgcg gcttcaacgt cttcgatgac ggcagcgccg agggcagcgc
     2881 gctgctcaag cacgcgatcg ccggcatggt cacgaccatg cgcgagacga tggcgctcta
     2941 cgccccgaac accaattcct accgccgctt ccagcccggc agccacgcgc cggtttcggc
     3001 gaactggggc tacgacaacc gcgccgcggc gctgcgcatt ccgtccggcg agcggcaggc
     3061 gatgcgcatc gaacaccgcc tcgccggcgc cgacgccaat ccgtatctgg cgctggcggc
     3121 gatgctggcc gggggcctgc gcggcatccg cgagaagctc gatcccggtg agcccagtcg
     3181 cggccatgtc ggcaacggcc aggcgccggt gctgccggcc tcgctgaagc acgcgctgga
     3241 aatcttcgac aagtccccgt tcccgcgcga agccttcggc gactcctacc cgcgcctcta
     3301 cagcgcctgt cgctggcagg aactggagaa gttcggcaag caggtcacgc cgctggaata
     3361 tgattcgtac ctgagaacgg tttgatgcgg atttatggtt tgcaggcagc tccggacagg
     3421 ctttgcgggg tcggattccg gttgtcatga cccgtctgag cagcgaggat gcggaaatgg
     3481 ctcatgaaaa gaagattgca cacctgacct tcattcaggg catcatcacc cggatgggca
     3541 ataattcctt tttgctcaag ggctggaccg ttaccttggt tgcggccttg tttgcacttg
     3601 cagccaagga tagtaatcag aatttcatac tgattgctta ttttcccatc cttgtgttct
     3661 ggatgctgga tggatatttc ctgtatcagg agcgtttgtt cagggacctt tacaatgggg
     3721 ttgccaacga caggatttcc agcgaacagt tcgagatcaa tcctgccagc ctcatgagcg
     3781 actctggtaa atggcagcca gcctttctct gggcacgagc tgcgctctcc aaaactttac
     3841 ttgtctttta catggcgatg atgggagtga cgctgttcgt catgtgcttc ctgctgcgtt
     3901 gagcgggcct gatcgaaaga gcggctgagg tgctggcggc cggcaggtgc ccggggacgg
     3961 gtggcgtgcc ggtcagtttt tctcatgcgt gcatcaacag tccttgttga tgtaggtcat
     4021 gaaaaatcgg taatgatcgt gccaccgtgt gcttgtgaag acctgcagca aaccggttgc
     4081 tgcggtgccg gcacaggccg acaggtgtcc gtcacggccg cacaggccgg cggagtgctg
     4141 ccgattcatc gcagccagtc atgccgcccc gccgatgagt accgattccg ccctgcctcc
     4201 ccggccggct gaaaccgggc aggatttcga aacttcagcc cctggccccc gtgccgcggg
     4261 caccgcgctg gccgtgcgcg atgcgctcgc gatcgacctg ctcgcgatct gcgagatata
     4321 cacgccctac gtgctctacg gcgcccagac cttcgaggaa gtgccgccgt cgctggcggc
     4381 gatgaccgag cgccgtgcgc aggtgctggc gcacggcctg ccgttcctgg tcgctgaacg
     4441 cggggcacgc gtgctcggct actgctacgc cacgccgtac cggtcacggc cggcctaccg
     4501 ctacaccgtg gaagactcga tctacatcgc ccacgactgc accgccggcg gcgtcggcac
     4561 ggcgctgctc gaagccctga tccggcgctg cgagcgcggg ccgtggcggc agatgatttc
     4621 ggtgatcggc gacagcgaca acgctgcgtc gatccggctg cacacccgcc acggcttcga
     4681 gccgatcggc atctgccggg cggtcggctt caagcacggg cgctggatcg acacggtgct
     4741 gatgcagcgc gcactcgggg ccggcgaccg gagcctgcca tcgggccggg cctgaagccg
     4801 cgacatcctg ccgcatgccg ggcgcgctac cggccggcta gactgcgccc ccctgcgccg
     4861 gcgacggcgc gaccggatcc ctgatgccga tgctgttctc cccggtcccc ccgacggccg
     4921 ccgaggccgc gcgcctgcat cgctaccggc tgctgctgat cgcgctggtg gccggggcgc
     4981 atgcgctcgc cgatgccgta ctcgggctcg acctgccgtg ggcgatgctg gcgctggtgc
     5041 tgggcggctg gctgctgctc gggcaggcgg cacgcgcgct gcggctggcg acgctgctgc
     5101 agttcggact cgacctcgtg ctgctcactg tcgtgttcgc caccaccggc ggcttcggca
     5161 acccgctggt gtcgctgtac ctgctgccgc tcgcactcgg cgcgacgcta ttgcgccgcc
     5221 ggctggcctg gatgctgacc gggctggcca tcggcatggc gacgctgctg atcgtggtct
     5281 ggacgccggc ggcggtcggc ctgcacacgg cgcacgccgc ccagcacccg cacgcgctgt
     5341 ccggcgggct cggctatgcc tcgcatctgg tcggcatgtg gctgagcttc gtcgccgccg
     5401 ccgggctgct cgtgcatttc accggcgcga tggccgtggt gctgcgccgg cgcgaacgcg
     5461 aactcgccgc tgcccgcgaa cagacgctgc gcgaccgtca ggtactggcc ctcggtgcgc
     5521 tcgccgccgg ggccgcgcac gaactcggca cgccgctcgc gaccatcgcc gtgctggcgc
     5581 gcgagctcga actcgatcac gccggcgatg ccgcgctcgc ggccgatctc gcaacgctgc
     5641 gcacacaggt cgagcactgc aagcacgcga tcagcagcct gctcgccgcc gccggtcagg
     5701 cgcgcgtcga ggccgccggc acgcaggcgc tcgatgtctg gctcgaaacc ctgctggcgc
     5761 gctggcagac gctgcgaccg gcagtgccgg tgcaactgca gctggccgcc ggcgcgtcgc
     5821 cgtcgatcct gaccgacgac accctcgccc agagcctggt cagcctgctc aacaacgccg
     5881 ccgatgtctc gccggccggc atcggcctgc gcgccgactg ggagggcggc ggcctgcgcc
     5941 tggccgtgct cgacgacggc cccggcctgc cggcacgggc cggcgaaccg cactggaccg
     6001 gcaagctgca cgggcgcgga ctcggcatct tcctcgcgcg ggccgcgatc gagcgcctcg
     6061 gtggtgaact gcgctacacg ctccgccccg aaggcggcac ctgcgccgaa atccggctgc
     6121 caaacgcggc gctggcggtg aacccgacat gagcgacgcc gccgccgacc ggccgacgct
     6181 gctgctggcc gatgacgacg acaccttccg cagcgtgctc gcccgcgcac tgacccggcg
     6241 tggttatgcg gtgcgccccg ccgccgatgt ggccgaagcg cgcaggctgg ccgccatcga
     6301 accgccggag ttcgcggtcg tcgatctgaa gatgccgggc gaatcgggac tggcgctggt
     6361 cgaggcgctg gtcgcgctcg acgccggcac gcgcatcatc gtgctgaccg gctacgcgag
     6421 catcacgact gcggtcgagg ccatcaagct cggtgccgtg cattacctcg ccaagcccgc
     6481 cgatgccgac cagatcgtcg ccgccttcgg ccgcctcgac ggcaatgccc gcgtgccggt
     6541 cagcgacacg ccgatgtcgg tcgatcgcct ggaatgggag cacatccagc gcgtgctcgc
     6601 cgaacacggc ggcaatgtct cggccaccgc ccgcgcgctg cacatgcacc gtcgcacgct
     6661 gcagcgcaag ctcggcaagc ggccggtg
//
LOCUS       DAOQIE010000224         6664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6121_length_6664_cov_5.279619, whole genome shotgun sequence.
ACCESSION   DAOQIE010000224 DAOQIE010000000
VERSION     DAOQIE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6121_length_6664_cov_5.279619"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK144_13290"
     CDS             <1..199
                     /locus_tag="PK144_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66747.1"
                     /translation="GLTVAFDPRLSPEAPAPAADAGPQLLYLPSGEATPFVLNLQAGG
                     RVQARLSGDALGRLQTEAVPP"
     gene            196..579
                     /gene="gspI"
                     /locus_tag="PK144_13295"
     CDS             196..579
                     /gene="gspI"
                     /locus_tag="PK144_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNF66748.1"
                     /translation="MSPRCPSSGGASSGFTLLEVVIAVLVIAIAFGGLLQALALQADR
                     LAEMRLRLLAGWVADNRIAEVLLAASAPVSGELHGETRQGGVALYWQQRSAPGGDANL
                     QRIDVEVGRAGETAVLARRSVLRER"
     gene            582..1196
                     /gene="gspJ"
                     /locus_tag="PK144_13300"
     CDS             582..1196
                     /gene="gspJ"
                     /locus_tag="PK144_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNF66749.1"
                     /translation="MSRRPSGYTLLELLVALAVFAVVITAAYAGVVQFLRTAQRLEAE
                     ARRDAEIQFAVDLFAQELAQAVARPVRDALGRREAALQLQGAQLLFTRTGWPAPPGQA
                     RTDLRRVLYRHDHGQWLRGEQAAPDRIDAAPPSTSVLLGGLDAWHLRCLTRDGQWLEH
                     WPPRGSDEASAQLPLAIEAVFEFTDRGSVRRLLTLVSTWPGPGS"
     gene            1193..2131
                     /gene="gspK"
                     /locus_tag="PK144_13305"
     CDS             1193..2131
                     /gene="gspK"
                     /locus_tag="PK144_13305"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNF66750.1"
                     /translation="MSAPRQRGIALLIVLIALLLVAGIATRLAWVGAQSLRRAGTAQH
                     LAEARLRGLSAEAWARSLLAQDGRTTTTDHPGEAWATLPPVLPFEGGELRGRIVDLGG
                     RFNLNTLLNADGSVNEEALALFQGLLAALDIDPQRSAAVLDWLDADAEPRPGGAEDAQ
                     YALLARPRATGNRPFVDFGELGAVQGFDAAMLARLAPHVTALPIATPINVNTASGLLL
                     GLLLGYGRGSAQAALQLEQARPLPGYASEQAFLAQRIAAAPRYPLALNSRCFRLEVQI
                     DLAGVRVMQRSLLIRPDEAGRQAPLVLWRSFDGMAP"
     gene            2128..3297
                     /gene="gspL"
                     /locus_tag="PK144_13310"
     CDS             2128..3297
                     /gene="gspL"
                     /locus_tag="PK144_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNF66751.1"
                     /translation="MKPAGTVIVRLRAAGLFEWQDGGGVHAGDAGAVAAALRRRRFVL
                     AIDTNELLLTEVPRLSRQRRLLAQALPAALEEMLAEDIDELHFATGPIEERLAVAVLR
                     RSRLIELLDAGWAAGLQPQAVLADVLLLPWRDGHWTLLHEDGQILLRCGRFRGYALSP
                     DELPALLELLQAEIGTGAHERPRLLLHGDALPPATIPPDWQFSPQGKAGFWQICTDVV
                     RDRLDLSAGLQLRPRAEPRNYWPVALLLGLWAGVHLYADWSGNQALAREEAVLQGRVG
                     DLFRSLAPDIRRMVQPRVQLEQRLRERERQGGQETGLLGLLGALAPLLEAGDAQLMAL
                     SYREGSLDCTLSGGSLDQLENLRRGLDRAGRFSTQLQSAQRDSRVEGRLTIRLKP"
     gene            3294..3779
                     /gene="gspM"
                     /locus_tag="PK144_13315"
     CDS             3294..3779
                     /gene="gspM"
                     /locus_tag="PK144_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNF66752.1"
                     /translation="MNAFWSRLSLRERWLLAGTGCIVLAAALYALAWAPLAAENAARR
                     QRIQALQADLAWMQQMAPRLQQAGARTSPVEDGNAQAPLPSRLETSLQRAGLGAGVRS
                     IEASSDARVTIAFESVAFDALIGWLEALQREQGITVGQAVLERLPAPGQVRARLEFGG
                     G"
     gene            3936..4580
                     /locus_tag="PK144_13320"
     CDS             3936..4580
                     /locus_tag="PK144_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66753.1"
                     /translation="MFAAGGGFHVCNHRGTENVIDAEPYGCRSDRRGGVRRPYRNGSG
                     PALAPARPGPTGAALDEMAALLDHLQRVAHPRRAPALDALDRRYAQRWLAYADLLETQ
                     LAVQEAGVPDGLLARAHVPVILAGVRDGSLQTQGDVQQHCGLKEANASRVLALLDAAE
                     LVLRQWHGREKRLMAGPRLPEALQLLEDAGRLPLATTPARTATSRRGSSFFKAA"
     gene            4584..>6664
                     /locus_tag="PK144_13325"
     CDS             4584..>6664
                     /locus_tag="PK144_13325"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNF66754.1"
                     /translation="MQTHITTFYSFKGGVGRSLLLANVGALLAQQAAPVLLWDLDLEA
                     PGLHHIPALRPKPVPKAGFLEWLQGRGERLGQSPSAAELNRLWKSVKPVPGREGLFIL
                     PACGDKADFAGLYQQIAWQGPMVEQPEQGLNLFRSLLAHLAEKGGFAHLLLDARTGVT
                     DLGGLMAAVLPHATVLVGGYGRQNRAGLLQVYRALQPAVDNRLAERGSLPPLARLLVT
                     SPVPDLPERRDECRRSWREDFVLAPEATQFEIPFDGRLLFEERLLAIEEPTSPIGLAY
                     QQIADALAELRDESQRTQKQASIAAETHDDPRWLGAERVVRGKRFEERVARLLTLLDY
                     RVEPEQYLDNANKVDLIARRTAGLRQECFLVECKDWEKPVPKAELERFRSWLNGPQAL
                     QMRAEGLYVARSYTRDALSYAKSQGLIAVTPQELEAQLFDFRPYLDRLRRRFEESPLA
                     RHYVAQRVLLESEPELKDGVDLIEHAQAWANGAGRRLWLVLGDYGTGKSSFFKRFAYA
                     LATRAIQEPDAPVPLAIDLKDYPNAISLEALLQEHLRKELNWHGNPEILLYLLAAGRV
                     TLLLDAFDEMGTAALARSVEAQFRQLVRPTAQVGEGARCNRVLITCRTHFFRDQQQIK
                     DVAGGHADDLVSSDSPLGQLARQFDASIDEVMLFNDAQIAEFLCRHLGEEKSQEALAF
                     IRRTYDLESLAP"
BASE COUNT          958 a         2223 c         2437 g         1046 t
ORIGIN      
        1 agggctgacg gtggccttcg atccgcgcct gagccccgaa gcgccggcgc cggctgccga
       61 tgccggcccg cagctcctgt acctgccaag cggcgaggcg accccgttcg tgctgaacct
      121 gcaggccggc ggacgggtgc aggcgcgcct gtccggcgat gcactcgggc gcctgcagac
      181 cgaggccgtg ccgccgtgag cccccggtgc ccctcgtccg gcggtgcctc gtccggcttc
      241 acgctgctcg aagtcgtgat cgcggtgctc gtgatcgcga tcgctttcgg cggcctgctg
      301 caggcgctgg cgctgcaggc ggaccggctc gccgaaatgc gcctgcgcct gctggcgggc
      361 tgggtggccg acaaccgcat cgccgaagtg ctgctcgcgg cgtcggcgcc cgtgagcggg
      421 gagctgcacg gcgaaacccg gcagggcggc gttgcgctgt actggcaaca gcgcagcgcg
      481 ccgggcgggg atgcgaacct gcagcgcatc gacgtcgagg tggggcgcgc cggggaaacc
      541 gccgtgctgg cccggcgcag cgtgctgcgc gagcgctgac cgtgtcgcgg cgtccctcgg
      601 gctacacgct gctggaactg ctcgtggcac tggccgtgtt cgccgtggtc atcaccgccg
      661 cctatgccgg ggtcgtgcag ttcctgcgca ccgcgcagcg cctcgaagcc gaggcccggc
      721 gcgatgccga aatccagttt gccgtcgatc tgttcgccca ggagctggcc caggccgtgg
      781 cccgccccgt ccgcgatgcc ctggggcggc gcgaggcggc gctgcagctg cagggggcgc
      841 agctgctgtt cacgcgcacg ggctggccgg ctccgcccgg tcaggcgcgc accgacctgc
      901 gtcgcgtgct ctatcgccac gatcacgggc agtggctgcg cggggaacag gctgcgcccg
      961 atcgcatcga tgctgcgccg ccatcgacga gcgtgctgct cggcggcctc gatgcctggc
     1021 atctgcgctg tctgacgcgc gatggccagt ggctggagca ctggccgccc cgcggcagcg
     1081 atgaagcctc ggcgcaactg ccgctcgcga tcgaggcggt gttcgagttc accgaccggg
     1141 gcagcgtccg ccggctgctc acgctggtat cgacctggcc ggggcccgga tcatgagtgc
     1201 gccacgccag cgcggcatcg cgctgctgat cgtgctgatc gcgctgctgc tggtcgcggg
     1261 gatcgccacg cggctggcct gggtcggtgc ccagagcctg cgccgggccg gcacggccca
     1321 gcatctggcc gaagcccggc tgcgcggcct gagcgccgag gcctgggcgc gcagcctgct
     1381 cgcgcaggac ggtcgcacca ccaccaccga tcacccgggc gaggcctggg caacgctgcc
     1441 gccggtgctg ccgttcgaag gcggcgagct gcgcggacgc atcgtcgatc tcggcgggcg
     1501 gttcaacctc aacacgctgc tcaatgccga cggctccgtg aacgaggagg cactggcact
     1561 gttccagggg ctgctcgcgg cgctcgacat cgatccgcag cgcagcgcag ccgtgctcga
     1621 ctggctcgac gccgacgcag aaccccgccc cggcggtgcc gaggatgccc agtacgccct
     1681 gctcgcccgg ccgcgcgcga ccggcaaccg gccgttcgtc gactttggcg aactgggcgc
     1741 ggtgcagggc ttcgatgccg cgatgctggc gcgtctggcc ccgcacgtga cggcgctgcc
     1801 gatcgcgacg ccgatcaacg tcaacacggc cagcggcctg ctgctcggcc tgctgctcgg
     1861 ctacggccgg ggcagcgcgc aggcggccct gcagctggag caggcccggc cgctgccggg
     1921 ctatgcgagc gagcaggcgt ttctggcgca gcgaatcgcc gccgcgcccc gctatccgct
     1981 ggccctcaac agccgctgct tccggctgga ggtgcagatc gacctggccg gggtgcgcgt
     2041 gatgcagcgc agcctgctga tccgccccga tgaagccggc agacaggccc cgctcgtgct
     2101 ctggcgcagc ttcgacggca tggccccatg aagccggccg gcaccgtgat cgtccggctg
     2161 cgcgccgccg gcctttttga atggcaggac ggcggcggcg tgcatgccgg cgatgccggg
     2221 gccgtcgccg ccgccctgcg gcggcggcgc ttcgtgctcg ccatcgacac gaacgaactg
     2281 ctgctgaccg aagtcccgcg gctgagccgc cagcggcgcc tgctggcgca ggccctgccg
     2341 gcggcgctcg aggaaatgct cgcagaggac atcgatgagc tgcacttcgc caccggaccg
     2401 atcgaggagc gtctggccgt ggccgtgctg cgccgcagcc ggctgatcga gctgctcgat
     2461 gccgggtggg cggcggggct gcagccgcag gcggtgctgg ccgacgtgct gctgctgccc
     2521 tggcgggacg ggcactggac gctgctccat gaggacgggc agatcctgct gcgctgcggg
     2581 cgctttcgcg gctatgcact cagccccgac gaactgccgg cgctgctgga actgctgcag
     2641 gccgaaatcg gcaccggcgc gcacgagcgg ccgcggctgc tgctgcacgg cgatgccctg
     2701 ccgcccgcga cgattccgcc ggactggcag ttctcgccgc agggcaaggc aggcttctgg
     2761 cagatctgca ccgacgtggt acgggaccgc ctcgacctga gcgccggcct gcagctccgg
     2821 ccccgggcgg aaccgcgcaa ctactggccc gtggccctgc tgctcgggct gtgggccggg
     2881 gtgcatctgt atgccgactg gagcgggaac caggcgctgg cccgcgagga agccgtgctg
     2941 cagggccgcg tcggggacct gttccgcagc ctggcgcccg atatccggcg catggtccag
     3001 ccgcgcgtgc agctcgaaca gcgcctgcgc gagcgtgaac ggcagggtgg acaggaaacc
     3061 ggcctgctcg gcctgctcgg tgcgctcgcc ccgctgctcg aagccggcga tgcgcagctg
     3121 atggccctca gctatcgcga gggcagcctc gactgcacgc tgagcggcgg cagtctcgat
     3181 cagctggaaa acctgcgccg cggcctcgac cgggcgggcc ggttcagcac gcagctgcag
     3241 tccgcgcagc gcgacagcag ggtcgaagga cgcctgacga tccggttgaa gccatgaacg
     3301 ctttctggtc acggctttcg ctgcgcgaac gctggctgct ggcggggacg ggatgcatcg
     3361 tgctcgccgc ggcgctgtat gccctggcct gggctccgct tgcagccgaa aacgccgccc
     3421 ggcgccagcg catccaggca ttgcaggccg atctggcgtg gatgcagcag atggccccgc
     3481 gcctgcagca ggccggtgcg cggacatccc cggtcgaaga cggtaacgcc caggcccccc
     3541 tgccgagccg cctggaaacc agcctgcagc gtgccggcct cggcgccggg gtccgcagca
     3601 tcgaggcgag cagtgatgcg cgtgtcacga tcgcgttcga atccgtcgcc ttcgatgcgc
     3661 tgatcggctg gctcgaagcc ttgcagcgcg agcaggggat taccgtcggg caggcggtac
     3721 tggagcgttt gccggccccg gggcaggtgc gggcgcggct tgaattcggg ggtggatgag
     3781 agggcaggcc cgtccgtctg tctcgccggc gatcgagttc ttgttcccgt ctgccggtca
     3841 ggcacaggcc gggtcatggc atcaacgcga tgaagcgcgc cggcggcaac ccgctcttcg
     3901 ggtaccagag ggcgacgggg ccgagagtca ggcggatgtt cgccgccggc ggcggatttc
     3961 atgtgtgtaa ccatcgaggc acggagaacg tgattgatgc tgagccgtac ggttgtcgat
     4021 cagatcgtcg cggcggcgtg cgccggccat accgcaatgg ctctggtccg gctctggcac
     4081 cagctcgacc gggaccgacc ggcgccgcgc tcgacgagat ggcggcgctg ctcgatcacc
     4141 tgcagcgggt ggcgcacccg cggcgtgcgc cggcactcga tgcgctggac cggcgctatg
     4201 cgcagcgctg gctggcctat gccgatctgc tcgaaaccca gcttgcggtg caggaagccg
     4261 gtgtgccgga cggcctgctg gcccgagcgc atgtgccggt gatcctcgct ggcgtgcgcg
     4321 acggcagtct gcaaacccag ggcgatgtgc agcagcactg cggcttgaag gaggccaatg
     4381 ccagccgtgt gctggcattg ctcgacgccg ccgaactggt gctgcgccag tggcacgggc
     4441 gcgagaagcg gctgatggcc ggcccgcgtc tgcccgaagc cttgcagttg ctggaggatg
     4501 ccggccggct gccacttgca acgacaccgg cacgcacagc gacttcacgg cgcggcagtt
     4561 cattcttcaa ggcggcctga gcggtgcaga cccacatcac gaccttctac agcttcaagg
     4621 gcggcgtcgg tcgcagtctg ctgctggcca acgtcggcgc gctgctcgca caacaggcag
     4681 cgccggtgct gttgtgggat ctggacctcg aagcgccggg cctgcatcac attccggcgc
     4741 tgcgtccgaa accggtgccg aaagcggggt ttctggaatg gctgcagggg cgcggtgagc
     4801 ggctcggtca aagcccgtcc gcggccgagc tgaaccggct gtggaagagc gtgaaacccg
     4861 tgccggggcg ggaggggctg ttcatcctgc cggcctgtgg cgacaaggcg gatttcgccg
     4921 ggctgtatca gcagatcgcg tggcagggac cgatggtcga gcagcccgag caagggctga
     4981 acctgttccg gtccctgctc gcgcatctgg cggagaaggg cggtttcgcg catctgctgc
     5041 tcgatgcccg taccggtgtg accgatctcg gcgggctgat ggccgcggtg ctgccgcatg
     5101 cgacggtgct ggtcggcgga tacggccggc agaaccgcgc cggtttgctg caggtgtacc
     5161 gcgcgctgca gccggcggtg gataaccgcc tggcggagcg gggcagtctg ccgccgctgg
     5221 cgcgtttgct ggtgacttcg ccggtgccgg atttacccga gcggcgggac gaatgccggc
     5281 ggagctggcg cgaggatttc gtgctggcgc cggaggcgac gcagttcgag attccgttcg
     5341 acggccggct gctgttcgag gaacggctgc tggcgatcga agagccgact tccccgatcg
     5401 ggctggctta tcagcagatt gccgatgcac tggccgaatt gcgggacgaa agccagcgca
     5461 cgcagaaaca ggcgagtatc gctgccgaga ctcatgacga tccgcgctgg ctcggtgccg
     5521 agcgggtggt gcgcggcaag cgtttcgagg agcgggtcgc tcgcctgctg acgctgctcg
     5581 attaccgggt cgagcccgag caatacctcg acaatgccaa caaggtcgat ctgattgccc
     5641 gcaggacggc cggtctgcgg caggaatgct ttctggtcga atgcaaggat tgggagaagc
     5701 cggttccgaa agcggaactg gagcgattcc gctcgtggct gaacggtccg caggctctgc
     5761 agatgcgggc cgagggcttg tatgtcgcgc gctcctatac gcgcgatgcc ctgagttacg
     5821 cgaaatccca gggcttgatc gccgtcacgc cgcaggagct ggaagcgcaa ctgttcgact
     5881 tccggcctta tcttgatcgc ttgcgccggc gtttcgagga aagcccgctg gcgcggcatt
     5941 acgtggcgca gcgggtgttg ctggaaagcg agccggagct gaaagacggc gttgacctga
     6001 tcgagcacgc gcaggcctgg gcaaacggtg ccgggcggcg cttgtggctg gtgctcggcg
     6061 attacggcac cggcaagtca tctttcttca aacgctttgc gtatgcactg gcgacgcggg
     6121 cgattcagga gccggatgcg ccggtgccgc tggcgatcga tctgaaggac tatccgaacg
     6181 cgatctcgct cgaagccttg ctgcaggaac atttgcgcaa ggaactcaac tggcacggca
     6241 atccggaaat cctgctctat ctgctcgcgg ccgggcgggt gacgctgctg ctcgatgcct
     6301 tcgacgaaat gggcacggcg gcgctggcgc gcagcgttga ggctcagttc cggcaactgg
     6361 tgcggccgac ggcgcaggtg ggggaaggcg cgcgttgcaa ccgggtgctg atcacctgcc
     6421 gcacgcattt cttccgcgac cagcaacaga tcaaggatgt cgccggtggc catgccgatg
     6481 atctggtcag cagcgattcg ccactcgggc aactggcccg gcaattcgat gccagcatcg
     6541 acgaggtgat gctgttcaac gacgcgcaga ttgcggaatt cctgtgccgg catctgggcg
     6601 aggaaaaatc gcaggaggca ctggctttca ttcgccggac ctacgatctg gaaagcctgg
     6661 cgcc
//
LOCUS       DAOQIE010000225         6655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6139_length_6655_cov_7.139848, whole genome shotgun sequence.
ACCESSION   DAOQIE010000225 DAOQIE010000000
VERSION     DAOQIE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_6655_cov_7.139848"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1232
                     /locus_tag="PK144_13330"
     CDS             12..1232
                     /locus_tag="PK144_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNF66755.1"
                     /translation="MSPTRSAAAAPPASRGARACAALLLACSSAALSPALAAGCVAPG
                     TWARPQDGAAETLDPREWYARLAGARVVLLGEHHDSVEQHRWQLQVIAGLHALRPQLV
                     IGLEMLPRRVQPVLDRWVAGGFDSEAEFLRAVDWDEVWRFDAARYQYLPILHFARMNR
                     VPLLALNVERTLLKRVGSEGWAAIPPAAREGVGDPARPAPAYLERLREAWRQHARSGS
                     NEPDEPAFLRFVDGQLLWDRAMAEALAARASRDSDAPLLVALLGAGHVRDGLGVPYQL
                     DALGVDGVVKLLTIGPEPCTGLQAGTADALFGLAGDGTAGPAPLMLGVVLATAAGTGV
                     RIERVLDGSLADTAGLQAGDLIRSVAGHPVHNPAEVAARVRPTAPGTWLPVEIERGGR
                     RRAVLVRVPPEPGE"
     gene            1229..3259
                     /locus_tag="PK144_13335"
     CDS             1229..3259
                     /locus_tag="PK144_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNF66756.1"
                     /translation="MTGGARLRQAAGALLLILSLPAPAATLLLDVRLDPQARLLEGDA
                     QFDPPTPDFLLGARFSPEPPAAWRRLGDDGQPLPPVADPDAAAAGAAADTVYRWRRLD
                     GARIARMRYSGRLDPLPAAADARSVLDARSPVADPAGSWLPAASRWFPARDDEPFLYR
                     LRLSLPGTQRGLVPGELLSAVSADGRWQAEFVAELPLEGLDLFAGPYQIEERRLARAE
                     GGEPLLIRSWFTGAVEGLGAGYREAAAGYLDGYAQRFGAYPLSGYSIVSSPLPAGFAV
                     PGITYLGESVLRLPFIRTTSLRHEVLHAWWGNGVRPDYARGNWCEGLVTLIADHDGRD
                     EETPQSALALRIDWLRDLAALGPEADYPLRAFTSRRHGADQIVGYRKGAYVFLMLRER
                     IGAAAFDAALRELWRRWRGRTADWAVLQTLFEQASGQRLGEWLAQWLDRTGLPAPQLA
                     LAEASREAGGWRLRLRLTQPGQPWQLTLPVVIDTEDGAQSELTLALARPSDEFVTALA
                     VRPVAVRLDPQARTARRLETAELPPILRQLQIASGVQVLLGGKPEDWPQTLAGLGRWL
                     DRPANGDGHGPRIVVGPAAQIDAWLATQRLTRPEQVTGGSAQAWAMIDAAGQPLLLLS
                     AADDTDAAPLVEKLAHHGRYGWLTLAADGSVAGRGQWPAPQRAVPVRISGVP"
     gene            3285..3746
                     /locus_tag="PK144_13340"
     CDS             3285..3746
                     /locus_tag="PK144_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66757.1"
                     /translation="MQEGSRLPPLPGVVAMDALCSALLLFLTEAGLTAAPAPPCPALS
                     VVAPATLAVRACEGPCPGLLAWYEAGARRIYLVAGFDADDPLQASVLLHELVHYLQDL
                     RGGFSDGSCRAGLLREVEAFRWQERWLARAGAWQPVGMALMNYGCATPAAG"
     gene            3871..5157
                     /locus_tag="PK144_13345"
     CDS             3871..5157
                     /locus_tag="PK144_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016952742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNF66758.1"
                     /translation="MIVLLLIFLNGLFAMSELAVVSARRARLQVLAEQGSKGAVAALK
                     LTEHPGRFLSTVQIGITLIGIFAGAYGGATLSEPLAAWIAEHHPALADSADTLALAIV
                     VALITYLSLIVGELVPKQLALRNPERIAVLAAPPMTVLAFLAAPIVHLLDLSANLGLR
                     LFGAEAQTEQKVTDEEIRTLLKEATEAGVVEHSEQAMIQGVMRLADRPVKALLTPRPD
                     IVWLDPADADAENIATLRASPYSRYPVCRGSVDEILGVVQAKDLLDDVLAGKPLDLRA
                     CLREAPVVHEDASALGLLETLRTSPLPLAFVVDEYGDLVGMVTATDVLESIVGDMAEH
                     DGSVEPDVVVREDGSWLLDGGLPIDELKELLRLRELPDEDEFNTLAGYLLARLGHLPS
                     TGEHMESDGYRFEIVDMDGRRIDKVLVAALPPEGSA"
     gene            5347..6585
                     /locus_tag="PK144_13350"
     CDS             5347..6585
                     /locus_tag="PK144_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002540892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNF66759.1"
                     /translation="MTTERLNPPGIVPLDHILPDEPLLMMGAGPVPIPHRVAQANSIV
                     INHLGETMARVVEQVKSMARYVFQTTSGHVMGVAGPGSAAMEMAVCNLVAPGTPVLAV
                     VNGYFSQRLAEMAGRVGGEVTTVHVDGNRCATPEDIGALLASGRFEVMTIVQGETSNT
                     VCNKRLPELARIARAHGCLVVVDAVCTLSTMPLDMDGWQIDAVITGGQKGLSSIPGVS
                     LLAFSDLAWERIRSRTHRLAHWCLDAQLADNFWNQKSYHYTAPVSGLLALHEALRLVC
                     EETLPQRFLRHLHCSEALQAAISAMGLDLFVSPESRLNSVIGIRVPDGVIADEVRRTM
                     STVHRVEISGAFGHNILRIGQMGEQSRAHNLFRTLHAFGASLRACGAALDLPAGMAEL
                     ERHLSSTTRLRVAERLGIAA"
BASE COUNT          859 a         2501 c         2341 g          954 t
ORIGIN      
        1 ccgaggtaac cgtgtccccg acccgatccg ccgccgccgc gccaccggca tcgcgcggcg
       61 cgcgcgcctg cgccgcgctg ctgctcgcct gcagcagtgc ggcgctctcc cccgccctgg
      121 ccgccggctg cgtggcaccg ggcacctggg cccgtccaca ggacggcgct gccgaaacgc
      181 tcgacccccg cgagtggtac gcccgcctcg ccggcgcgcg cgtcgtgctg ctcggcgaac
      241 accacgacag cgtcgaacag caccgctggc agctgcaggt catcgccggc ctgcacgcgc
      301 tgcgcccgca gctcgtgatc gggctggaga tgctgccgcg gcgggtgcag ccggtgctgg
      361 atcgctgggt ggccggcggt ttcgacagtg aggccgagtt cctgcgcgcc gtcgactggg
      421 acgaggtctg gcgcttcgac gccgcccgct accagtacct gccgatcctg cacttcgcgc
      481 gcatgaaccg tgtgccgctg ctggcgctga acgtcgagcg cacgctgctc aagcgcgtcg
      541 gcagcgaggg ctgggccgcg atcccgccgg cggcgcgcga aggggtcggc gatccggccc
      601 ggccggcgcc ggcgtatctc gaacggctgc gcgaagcctg gcgccagcac gcccgctcgg
      661 gcagcaatga acccgacgaa cccgccttcc tgcgcttcgt cgacggccag ctgctgtggg
      721 atcgcgcgat ggccgaagcc ctggccgcac gcgcaagccg tgacagcgac gccccgctgc
      781 tggtcgcgct gctcggtgcc ggccacgtgc gcgatggcct cggcgtgccc taccagctcg
      841 acgcgctcgg ggtcgacggg gtcgtgaagc tgctgaccat cggcccggag ccctgcaccg
      901 gcctgcaggc cggcacggcc gatgcgctgt tcggcctcgc cggcgacggc acagccgggc
      961 cggcgccgct gatgctcggc gtcgtgctcg ccaccgccgc cggcaccggc gtgcgcatcg
     1021 agcgggtgct cgatggcagc ctcgccgata ccgccggcct gcaggccggt gacctgatcc
     1081 gcagcgtcgc cggccacccc gtgcacaacc ccgccgaggt cgccgcgcgc gtgcgtccga
     1141 ccgcgcccgg cacctggctg ccggtcgaga tcgagcgcgg cggccgccgg cgggcggtgc
     1201 tggtgcgcgt gccgccggag cccggggaat gacgggcggc gcacggctgc gccaggcggc
     1261 cggagcgctg ctgctgatcc tgtcactgcc ggctccggct gcgacgctgc tgctcgacgt
     1321 gcgcctcgat ccgcaggcac ggctgctgga aggcgatgcg cagttcgatc cgccgacccc
     1381 ggacttcctg ctcggcgcgc gcttctcgcc cgagccgccg gccgcctggc gccggctcgg
     1441 cgacgacggc cagccgctgc cgccggtcgc cgaccccgac gcggccgccg ccggcgcagc
     1501 ggccgatacc gtgtaccgct ggcgccggct cgacggcgcg cgcatcgcgc gcatgcgtta
     1561 cagcggccgg ctcgatccgc tgccggccgc tgccgatgcc cgcagcgtgc tcgatgcccg
     1621 gtcgccggtg gccgatccgg ccggcagctg gctgccggcg gcgagccgct ggttcccggc
     1681 ccgcgacgac gaacccttcc tgtaccggct gcggctgtcg ctgcccggca cccagcgtgg
     1741 cctggtgccg ggcgagctgc tgagcgccgt gtcggcggac ggccgctggc aggccgagtt
     1801 cgtcgccgag ctgccgctgg aaggactgga tctgttcgcc ggtccctacc agatcgagga
     1861 gcgccggctg gcgcgcgccg agggcggcga gccgctgctg atccgcagct ggttcacggg
     1921 cgcggtcgaa gggctgggcg ccggctatcg cgaggcggcg gccggttacc tcgacggcta
     1981 tgcgcagcgc ttcggcgcct atccgctgtc cggctattcg atcgtgtcct cgccgctgcc
     2041 ggccggcttc gcggtgccgg gcatcacgta tctcggcgaa tcggtgctgc gcctgccgtt
     2101 catccgcacc acctcgctgc gccacgaggt cctgcatgcg tggtggggca acggcgtgcg
     2161 tcccgactac gcgcgcggca actggtgcga ggggctggtg acgctgatcg ccgatcacga
     2221 cggccgtgac gaggaaaccc cgcagagcgc gctggcgctg cgcatcgact ggctgcgcga
     2281 cctcgccgcg ctcggccccg aggccgacta cccgctgcgt gccttcacca gccgccgcca
     2341 cggcgccgac cagatcgtcg gctaccgcaa gggcgcctac gtgttcctga tgctgcgcga
     2401 gcgcatcggt gccgcggcct tcgatgccgc gctgcgcgag ctgtggcgcc gctggcgcgg
     2461 gcgcacggcc gactgggcag tgctgcagac gctgttcgaa caggcttcgg gccagcggct
     2521 cggcgagtgg ctggcgcagt ggctggaccg caccggcctg ccggccccgc agctcgcgct
     2581 cgccgaagcc agccgcgagg ccggcggctg gcggctgcgg ctgcggctga cgcagcccgg
     2641 tcagccctgg cagctgacgc tgccggtggt gatcgacacc gaggatggcg cccagtcgga
     2701 gctgacgctg gcgctggcgc gccccagcga cgaattcgtg accgcactcg ccgtgcgccc
     2761 cgtcgcggtg cggcttgatc cgcaggcgcg cacggcccgc cggctcgaaa ccgcggaact
     2821 gccgccgatc ctgcgccagc tgcagattgc gagcggcgtg caggtgctgc tcggcggcaa
     2881 gcccgaggac tggccgcaga cgctggccgg cctcggccgc tggctcgacc gcccggccaa
     2941 cggcgacggg cacgggccgc gcatcgtggt cggcccggcc gcgcagatcg acgcctggct
     3001 ggcaacgcag cggctgacgc gcccggaaca ggtcacgggc ggcagcgccc aggcctgggc
     3061 catgatcgat gccgccggcc agccgctgct gctgctgtcg gcagccgatg acaccgacgc
     3121 cgcgccgctg gtcgaaaagc tcgcgcatca cggccgctac ggctggctga cgctcgctgc
     3181 cgatggcagc gtcgccgggc gtggccagtg gccggcgccg cagcgcgccg tgccggtacg
     3241 catcagcggc gtgccctgaa ccaccgcgct ggtgcgcaag ctgcatgcag gaaggcagtc
     3301 gtctgccacc gctgccgggg gtcgtcgcga tggatgccct gtgctctgcc ctgctcctgt
     3361 tcctgaccga agccggtctc accgccgcgc ccgcgccgcc ctgcccggcg ctgagcgtgg
     3421 tcgccccggc aacgctggcg gtgagggcct gcgaagggcc ttgtccgggt ctgctggcct
     3481 ggtacgaggc cggggcccgg cgcatctacc tcgtggccgg cttcgatgcg gacgatccgc
     3541 tgcaggcgag cgtgctgctg cacgaactcg tgcattacct gcaggacctg cgcggcggtt
     3601 tcagcgatgg cagctgccgc gccgggctgc tgcgcgaggt cgaggccttc cgctggcagg
     3661 agcgctggct ggcgcgcgcc ggcgcctggc agccggtcgg catggcactg atgaactacg
     3721 gctgcgcgac gcccgcggcc ggatgagtcc cggggtccgg cgcatcgcca tgacctgcga
     3781 acatgacgcc gccagccgcg gcccgctaag atcgccggcg ttgccgtccg gcaacgctga
     3841 cgccacccat cgatgattct ctgggagctg ttgatcgtcc tgctgctgat ctttctgaac
     3901 ggcctgttcg caatgtcgga actcgcagtg gtctcggccc gccgggcccg gctgcaggta
     3961 ctcgccgagc agggcagcaa gggcgccgtc gcggcgctga aactcaccga acaccccggc
     4021 cgcttcctgt ccaccgtgca gatcggcatc acgctgatcg gcatcttcgc cggcgcctac
     4081 ggcggcgcga cgctgtcgga accgctcgcc gcgtggatcg ccgagcatca tccggcgctc
     4141 gccgactcgg ccgacacgct ggcgctcgcc atcgtcgtgg cgctgatcac ctatctgtcg
     4201 ctgatcgtcg gcgaactcgt gccgaagcag ctggcactgc gcaatcccga gcgcatcgcc
     4261 gtgctcgccg cgccgccgat gacggtgctc gccttcctcg ccgcgccgat cgtgcatctg
     4321 ctcgacctgt cggccaacct cggcctgcgg ctgttcggcg ccgaggcgca gaccgagcag
     4381 aaggtcaccg acgaggaaat ccgcacgctg ctcaaggaag ccaccgaggc cggcgtcgtc
     4441 gagcacagcg agcaggcgat gatccagggc gtcatgcgcc tggccgaccg gccggtgaag
     4501 gcgctgctga cgccgcgccc ggacatcgtc tggctcgacc cggccgatgc cgatgccgag
     4561 aacatcgcca cgctgcgcgc gagtccgtac tcgcgctacc cggtctgccg cggttcggtc
     4621 gacgagatcc tcggcgtcgt gcaggccaag gacctgctcg atgacgtgct cgccggcaag
     4681 ccgctcgacc tgcgcgcctg cctgcgcgag gcgccggtcg tgcacgagga cgcgagcgcc
     4741 ctcggcctgc tcgaaaccct gcgcacctcg ccgctgcccc tggccttcgt cgtcgacgaa
     4801 tacggcgatc tggtcggcat ggtcacggcc accgacgtgc tggagagcat cgtcggcgac
     4861 atggccgagc acgacggcag cgtcgagccg gacgtggtgg tgcgcgagga cggctcctgg
     4921 ctgctcgacg gcggcctgcc gatcgacgag ctgaaggaac tgctgcgcct gcgcgaactg
     4981 cccgacgagg acgaattcaa cacgctcgcc ggctacctgc tcgcccgcct cggccacctg
     5041 ccctccaccg gcgagcacat ggaaagcgac ggctaccgct tcgagatcgt cgacatggac
     5101 gggcggcgca tcgacaaggt gctggtcgcc gcgctgccgc ccgaaggcag cgcctgaccc
     5161 gcgccctctg cccggtgccg gtgcatcgcc gtgcagcaca tgcctgccga agcggcgcac
     5221 gtcgcaccca cccggtgcat cgggggcgga tttccagtaa gctcccgcgc cttttagcaa
     5281 ttcggccgcc ggtgccgagc cgaccgccgc gccctttccc cgtcgctgcc tgcagacctc
     5341 gcccacatga cgaccgaacg cctgaacccg cccggcatcg tgccgctcga tcacatcctg
     5401 cccgatgagc cgctgctgat gatgggcgcc ggtccggtgc cgatcccgca ccgcgtcgca
     5461 caggccaact ccatcgtcat caaccacctc ggcgagacga tggcgcgcgt cgtcgagcag
     5521 gtgaagtcga tggcccgcta cgtgttccag accacgtccg gccacgtcat gggcgtcgcc
     5581 ggtccgggct cggcggcgat ggagatggcg gtctgcaatc tggtcgcccc cggcacgccg
     5641 gtgctcgcgg tcgtcaacgg ctacttcagc cagcgtctgg ccgagatggc cgggcgcgtc
     5701 ggcggcgagg tcacgaccgt gcacgtcgac ggcaaccgct gcgcgacgcc cgaggacatc
     5761 ggcgcgctgc tcgccagcgg ccgcttcgag gtcatgacca tcgtccaggg cgagacctcg
     5821 aacacggtct gcaacaagcg cctgccggaa ctggcacgca tcgcccgcgc acacggctgt
     5881 ctggtcgtcg tcgatgcggt ctgcacgctc agcacgatgc cgctcgacat ggacggctgg
     5941 cagatcgacg cggtgatcac cggcggccag aagggtttgt cctcgatccc cggcgtgtcg
     6001 ctgctggcgt tctcggatct cgcctgggag cgcatccgca gccgcacgca ccgcctcgcg
     6061 cactggtgcc tcgatgcaca gctcgccgac aacttctgga accagaagtc gtatcactac
     6121 accgcgccgg tctccggcct gctggcgctg cacgaggcgc tgcgtctggt ctgcgaggaa
     6181 accctgccgc agcgcttcct gcgccacctg cactgctccg aggcgctgca ggccgcgatc
     6241 agcgcgatgg gactcgatct gttcgtctcg cccgaatcgc ggctgaactc ggtgatcggc
     6301 atccgcgtgc cggacggcgt catcgccgac gaggtgcgcc ggaccatgtc gaccgtgcac
     6361 cgcgtcgaga tttccggcgc cttcggccac aacatcctgc gcatcggcca gatgggcgag
     6421 cagagccgcg cccacaacct gttccgcacc ctgcacgcct tcggcgccag cctgcgcgcc
     6481 tgcggcgccg cactcgacct gccggccggc atggccgaac tggagcggca cctgtcttcg
     6541 acgacgcgcc tgcgcgtcgc ggaacggctc gggatcgcgg cgtaagccga atcgcatcag
     6601 tcgcatcagt cgcatcaatc aggcccggcg tgcggagcaa gtgcgcacgc cgggc
//
LOCUS       DAOQIE010000226         6626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6190_length_6626_cov_8.066048, whole genome shotgun sequence.
ACCESSION   DAOQIE010000226 DAOQIE010000000
VERSION     DAOQIE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_6626_cov_8.066048"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PK144_13355"
     CDS             complement(<1..1021)
                     /locus_tag="PK144_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNF66760.1"
                     /translation="MGFQVSLQPGGSTFVVEAGEAVLDAALRAGCHIPYGCRGGSCGA
                     CAGRLLSGDYHYPQGLPPGLSPREAETGRVLLCQARARSDLVFELHELRMPPGIEVRT
                     LPARVVSMQRLADDVMRLYLKLPASENFRYLAGQYVDILLRGGGRRGFSIANRPGGED
                     FLELHVRLVPGGEFTTHVFAEMKERAILRLQGPLGTFFVREDSSRPLLMMAGGTGFAP
                     LKAMIEQIYASGPHTRPVHLYWGARHRAGLYLHELVEGWIDARPGLRYTPVLSEPLPE
                     DDWRGRTGWVHEALAADYPDLSGFDVYMSGPPAMVRAARERFAQQGLPDGQLCFDAFE
                     FAHTTS"
     gene            1234..1992
                     /locus_tag="PK144_13360"
     CDS             1234..1992
                     /locus_tag="PK144_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66761.1"
                     /translation="MHADTAALRRHVLARAPLCAPGDGPVLGLVFVTLFGALALDAAF
                     GMTGQVLGSLAVWALLLVLLARMTPAWRRTLVIATAFALAAELFFSLVLGWYDYQFLN
                     VPAFVPPGHVLLFLLGPALACRLPQWLVIAVPLAAGAWALWASLTGIGPFDGLLCAVF
                     LLAYRFGRSRPTYALMLLLALALELWGTGLGNWRWQPVVPGLGLATVNPPLLAGTFYA
                     LFDMYVMLSARACHRHFGDLPGAAQPGERSRMPP"
     gene            2082..4199
                     /locus_tag="PK144_13365"
     CDS             2082..4199
                     /locus_tag="PK144_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66762.1"
                     /translation="MTDSGQGNTAQGQAAMQEQRLRQIVDQATDTFIVIDAAGCIADV
                     NAHGCQNLGYSREQLIGRPYALLDPLQPPARLAELTTALATGPLTIETHHRRSDGSEL
                     PVEVRFGALATGDGPCLLAICRDISERRALEAQLRFQSTHDLLTGLPNRNLLEDRLNM
                     AVAHARRRSRLVGVLCLDLIRFKQLVDTLGHTATDELLRQIARRLIGVVRPGDTVSRQ
                     AGDTFVIVLNELVAEERASQIAGRLREAVTTPLTVAGQEVYPDASIGIAVFPRDGSDC
                     DTLLRHAAAAMQRARQHPRNEPQFYAAAMNTWATERLGLEGKLRRALDGRELLLHYQP
                     QIELSTGHIVGTEALLRWRHPELGMVSPAEFIPLAEETGLILPIGDWVLRTACAQARA
                     WRDAGLPELRMCVNLSALQFAQPDLAERVAAVLAETGLPATALELEITESVLMRDVEA
                     AAAMIGTLNDMGVQLALDDFGTGYSSLAYLKRFRIQRLKIDRSFVRDITTDPDDAAIT
                     LAVIALAHSLRKSVIAEGVETEEQRHFLSERGCDEVQGFYFSRPLPAEDLTELLADRR
                     ALANGLGGAETEEPPTVLLVDDDPVFTTFGCSVLRNEACRVLSARSGREALDLLARNR
                     VDVIVSDQVMPEMDGADFLRRTRALYPNACRIMLSGSTSLQSLISSINEGGIHKFLEK
                     PVTPERLRTTVREALARRTPPTG"
     gene            4336..6285
                     /locus_tag="PK144_13370"
     CDS             4336..6285
                     /locus_tag="PK144_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66763.1"
                     /translation="MKRRERSWPQRVLLRFLPWAAVTLLVAGFALERMERAALDPLEA
                     AQERAIVRGGRVLAAALGHLGRDIDILAHHDALLAAIADDGADPGHLQRLTELFHAFS
                     AASRDYDQIRWLDRTGRERVRVDLRDDEPYTIPAAELQLKADRPYFRDTIGLRPGEVY
                     LSPLDLNVERGAIELPHKPMLRLATPLFDERGAPRGVLVMNYLAGPVLAQLSRPTETG
                     TVAMLANTDGYWLVARDPRLSFGFQLGRPELTLAQQAPAVWAQMQQRDAGSLRAEGGL
                     WSWDTFRPSLRLRGETRVQDRWKIITFVPEDQLAARERMARWEVGAVTLLLLGLALFV
                     SIRLAQAACQNEQAQAALAERSRSLTQANEALAETNAELHATLSRLTRTQSELVQAEK
                     LASLGLMVAGVAHELNTPVGAALVTVSALDREAVRLAGQLKSGLKKSDLEAFLTHGVE
                     GRGIVTDNLQRAAALIRSFKQLATDRASTERREFTLDELVEDMLRTLHPRLKHLPHRI
                     ETDIPAGLHFDSYPGPLGQVVQNLIDNALAHAFHDHPHGTIRLRASAGAEDATEVRIE
                     VEDDGSGIPPEHLPRIFDPFFTTRRGRGGTGLGLHLSHQFAHGLLGGRLQVASRVGIG
                     TCFTLIVPRIAPAAPDAEPAAAPPA"
     gene            6282..6554
                     /locus_tag="PK144_13375"
     CDS             6282..6554
                     /locus_tag="PK144_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF66764.1"
                     /translation="MNCRPGCGACCIAPSISSPIPGMPVGKPAGVRCVQLDDQQRCRL
                     FGRPERPAVCASLRPEPAMCGADRAQALLRLARLEALTRPLDSQGP"
BASE COUNT          924 a         2528 c         2265 g          909 t
ORIGIN      
        1 cgctcgtggt gtgcgcgaac tcgaaggcgt cgaagcagag ctgcccgtcg ggcaggccct
       61 gctgcgcgaa gcgctcgcgc gcggcgcgca ccatcgccgg cgggccgctc atgtagacat
      121 cgaagcccga gaggtccggg taatcggcgg ccagcgcctc gtgcacccag ccggtgcgtc
      181 cgcgccagtc gtcttcgggc agcggctcgg acagcaccgg cgtgtagcgc aggcccggcc
      241 gggcgtcgat ccagccttcg accagttcgt gcaggtacag cccggcgcgg tggcgtgcgc
      301 cccagtacag gtgcaccggc cgcgtgtgcg gtccgctggc gtagatctgc tcgatcatcg
      361 ccttcagcgg cgcgaagccg gtgccgccgg ccatcatcag cagtggccgc gacgagtcct
      421 cgcgcacgaa gaaggtgccg agcggcccct gcaggcgcag gatcgcgcgc tccttcatct
      481 cggcgaacac gtgggtcgtg aactcgccgc ccggcaccag gcgcacgtgc agttcgagga
      541 aatcctcacc gcccgggcgg ttggcgatcg agaagccgcg ccggccgccg ccacgcagca
      601 ggatgtcgac gtactggccg gccagatagc ggaagttctc gctggccggc agcttcaggt
      661 acaggcgcat cacgtcgtcg gcgaggcgct gcatcgacac cacgcgcgcc ggcagcgtgc
      721 gcacctcgat gccgggcggc atgcgcagtt cgtgcagctc gaacacgaga tcgctgcgcg
      781 cccgcgcctg acagagcagc acgcgcccgg tctcggcctc gcgcggcgac agccccggcg
      841 gcaggccctg cggatagtgg taatcgccgg acagcagccg cccggcgcag gcgccgcagc
      901 tgccgccgcg gcagccgtac gggatgtgac aaccggcacg cagcgcagcg tcgagcaccg
      961 cttcgccggc ctcgaccaca aacgtgctgc cgcccggctg cagactgacc tgaaacccca
     1021 tccccgaccc ccgcctcacc aaaggccgcg catcttcgcc gatccggcgc cggtgcggaa
     1081 ccgcaccatg gcggccatgc tgcaccttgc agcaggccgt gaccttgcgc cagcgcaaat
     1141 ccggatgcgg agcgccggcg cacgcgcggc ttgccgcaaa cccgctggca acgcaggctg
     1201 acacctcctg ccgcccgccc ccatccgctc tccatgcacg ccgataccgc cgccctgcgc
     1261 cgccacgtcc tcgcccgcgc cccgctgtgc gcccccgggg acggccccgt gctcgggctg
     1321 gtgttcgtga cgctgttcgg ggcgctggcc ctcgatgccg cattcggcat gaccgggcag
     1381 gtgctcggca gcctcgcggt gtgggcgctg ctgctcgtgc tgctcgcgcg catgacgccg
     1441 gcctggcggc ggacgctggt gatcgccacg gccttcgcac tggccgcgga gctgttcttc
     1501 tcgctggtgc tcggctggta cgactaccag ttcctgaacg tgccggcctt cgtgccgccc
     1561 ggccacgtgc tgctgttcct gctcggtccg gcgctggcgt gccggctgcc gcagtggctg
     1621 gtgatcgccg tgccgctggc cgccggcgcc tgggcgctgt gggcgagcct caccggcatc
     1681 gggccgttcg acggcctgct gtgcgcggtc ttcctgctcg cctaccgctt cgggcgctcg
     1741 cggccgacct atgcgctgat gctgctgctg gcgctggcgc tggagctctg gggcaccggc
     1801 ctcggcaact ggcgctggca gccggtggtg ccgggcctcg ggctggcgac ggtcaatccg
     1861 ccgctgctcg ccggcacctt ctacgcgctg ttcgacatgt acgtgatgct gagtgcgcgt
     1921 gcctgccacc ggcacttcgg cgatctgccg ggcgcagcgc agccgggcga gcggtcacgc
     1981 atgccaccat agtgaccggt ccggcggctc gcggtgctac cctcatgcct ctggaggccg
     2041 cgccggtacg cccggtgcat ggctccgcat gcgaggacac cgtgacggat tccggacagg
     2101 gcaacacggc gcagggccag gcggcaatgc aggagcagcg gctgcggcag atcgtcgacc
     2161 aggccaccga caccttcatc gtcatcgacg ccgccggctg catcgccgac gtcaacgccc
     2221 atggctgcca gaacctcggc tactcgcgcg agcagctgat cggccgcccg tatgcgctgc
     2281 tcgacccgct gcagccccct gcccggctcg ccgagctcac taccgcgctg gcgaccgggc
     2341 cgctgaccat cgagacccac caccgccgca gcgacggcag cgaactgccg gtcgaggtgc
     2401 gcttcggcgc gctcgccacc ggcgacggcc cgtgcctgct cgcgatctgc cgcgacatct
     2461 ccgagcggcg cgcgctcgaa gcgcagctgc gcttccagag cacgcacgac ctgctgaccg
     2521 gcctgccgaa ccgcaacctg ctcgaagacc gcctgaacat ggcggtcgcg cacgcccgcc
     2581 gccgcagccg gctggtcggc gtgctgtgtc tcgacctgat ccgcttcaag cagctggtcg
     2641 acacgctcgg ccacacggcc accgacgagc tcctgcgcca gatcgcccgg cgcctgatcg
     2701 gcgtcgtgcg ccccggcgac accgtctcgc gtcaggccgg cgacaccttc gtgatcgtgc
     2761 tcaacgaact cgtcgccgag gagcgcgcca gccagatcgc cggacgcctg cgcgaggccg
     2821 tgaccacgcc gctgaccgtc gccggccagg aggtctaccc ggacgccagc atcggcatcg
     2881 ccgtgttccc gcgcgacggc agcgactgcg acacgctgct gcgccacgcc gccgccgcga
     2941 tgcagcgtgc ccgccagcac ccgcgcaacg agccgcagtt ctacgcggcg gcgatgaaca
     3001 cctgggcgac cgagcgcctc gggctcgaag gcaagctgcg ccgcgcgctc gacggccgcg
     3061 agctgctgct gcactaccag ccgcagatcg agctgagcac cgggcacatc gtcggcaccg
     3121 aggcgctgct gcgctggagg catccggagc tcggcatggt ctcgccggcg gagttcatcc
     3181 cgctcgccga ggagaccggg ctgatcctgc cgatcggcga ctgggtgctg cgcaccgcct
     3241 gcgcgcaggc ccgcgcctgg cgcgacgccg gcctgccgga gctgcgcatg tgcgtgaacc
     3301 tgtcggcact gcagttcgcg cagcccgatc tggccgagcg cgtcgccgcc gtgctcgccg
     3361 aaaccggcct gccggcgacg gcgctggagc tggagatcac cgaatcggtg ctgatgcgcg
     3421 atgtcgaggc cgccgcggcg atgatcggca cgctgaacga catgggcgtg cagctcgcgc
     3481 tcgatgactt cggcaccggc tattcctcgc tcgcctacct caagcgcttc cgcatccagc
     3541 ggctgaagat cgaccgcagc ttcgtgcgcg acatcaccac cgaccccgac gacgccgcga
     3601 tcacgctggc ggtgatcgcc ctcgcgcaca gcctgcgcaa gtcggtgatc gccgaaggcg
     3661 tcgagaccga ggaacagcgc cacttcctca gcgagcgcgg ctgcgacgag gtgcagggct
     3721 tctacttcag ccggccgctg ccggccgagg atctgaccga gctgctcgcc gaccgccgcg
     3781 ccctcgccaa cggcctcggc ggcgccgaga ccgaagagcc gccgaccgtg ctgctggtcg
     3841 atgacgatcc ggtgttcacg accttcggct gctcggtgct gcgcaacgag gcctgccgcg
     3901 tgctgagcgc ccgcagcggc cgcgaggccc tcgacctgct ggcgcgcaac cgcgtcgatg
     3961 tcatcgtctc cgatcaggtc atgccggaga tggacggcgc cgacttcctg cgccgcacgc
     4021 gcgcgctgta ccccaatgcc tgccggatca tgctgagcgg ctcgacttcg ctgcagagcc
     4081 tgatcagcag catcaacgag ggcggcatcc acaagttcct cgaaaaaccg gtgacgccgg
     4141 agcgcctgcg cacgacggtg cgcgaggcac tggcgcggcg cacgccgccg accggctgac
     4201 ggcgcggccc ccggctgtgg cagcatgccg tccgcgcacc gcccggtgcg ctgcagcaac
     4261 gccgggaggc gacgccatgg actgccggat actcaccgcc gccaccaccg tgtccctcgc
     4321 gccgggagcc tgtccgtgaa gcgccgcgag cggagctggc cgcagcgcgt gctgctgcgc
     4381 ttcctgccgt gggcggcggt gacgctgctg gtcgcaggct ttgcgctgga gcgcatggag
     4441 cgcgcagcgc tcgatccgct cgaagccgca caggagcgcg cgatcgtgcg cggcggccgg
     4501 gtgctcgcgg ccgcgctcgg acacctcggc cgcgacatcg acatcctcgc gcaccatgac
     4561 gcgctgctcg cggcgatcgc cgacgacggt gccgatcccg ggcacctgca gcggctgacc
     4621 gagctgttcc acgccttctc ggccgccagc cgcgactacg accagatccg ctggctggac
     4681 cggaccggcc gcgagcgcgt gcgcgtcgac ctgcgcgatg acgagccgta caccatcccg
     4741 gccgccgaac tgcagctgaa ggccgaccgg ccgtacttcc gcgacaccat cggcctgcgt
     4801 cccggcgagg tctacctgtc accgctcgac ctcaacgtcg agcgcggcgc gatcgagctg
     4861 ccgcacaagc cgatgctgcg cctggccacg ccgctgttcg acgagcgcgg cgcgccgcgc
     4921 ggcgtgctgg tcatgaacta cctggccgga ccggtgctgg cgcagctgtc acgaccgacc
     4981 gaaaccggca cggtcgcgat gctcgcgaac accgacggct actggctggt cgcccgcgat
     5041 ccgcgcctga gcttcggctt ccagctcggc cggccggaac tgacgctcgc gcagcaggcc
     5101 ccggcggtct gggcgcagat gcagcagcgc gatgccggca gcctgcgcgc cgaaggcggc
     5161 ctgtggagct gggacacctt ccgcccgtcc ctgcgcctgc gcggcgagac gcgcgtgcag
     5221 gatcgctgga agatcatcac cttcgtgccc gaggaccagc tcgcggcgcg ggagcgcatg
     5281 gcgcgctggg aagtcggcgc cgtgacgctg ctgctgctcg gactggcgct gttcgtgtcg
     5341 atccgcctcg cgcaggcggc atgccagaac gagcaggcgc aggccgcact cgccgagcgc
     5401 tcgcgctcgc tgacccaggc caacgaggcg ctggccgaga ccaatgccga gctgcacgcg
     5461 acgctcagcc gcctgacgcg gacgcagtcc gaactcgtgc aggccgagaa gctcgcctcg
     5521 ctcggcctga tggtcgccgg cgtcgcgcac gagctgaaca ccccggtcgg cgcggcgctg
     5581 gtgacggtct cggcgctcga tcgcgaggcc gtcaggctcg ccgggcagct gaaatccggc
     5641 ctgaagaaat ccgacctcga agccttcctc acccatggcg tcgagggacg cggcatcgtc
     5701 accgacaacc tgcagcgcgc cgcggcgctg atccgcagct tcaagcagct cgccaccgac
     5761 cgcgccagca ccgagcggcg cgagttcacg ctcgatgagc tggtcgagga catgctgcgc
     5821 acgctgcacc cgcgcctgaa gcacctgccg caccgcatcg agaccgacat tccggccggc
     5881 ctgcacttcg acagctaccc cgggccgctc ggtcaggtcg tgcagaacct gatcgacaac
     5941 gccctcgccc acgccttcca cgaccacccg cacggcacga tccggctgcg cgcgagtgcc
     6001 ggggccgaag acgcgaccga ggtgcgcatc gaggtcgagg acgacggttc cggcatcccg
     6061 cccgagcatc tgccgcgcat cttcgatccg ttcttcacga cccggcgcgg gcgcggcggc
     6121 accggcctcg gcctgcacct gtcgcaccag ttcgcgcacg gcctgctcgg cggccggctg
     6181 caggtcgcga gccgcgtcgg catcggcacc tgcttcacgc tgatcgtgcc gcgcatcgcc
     6241 ccggccgcac ccgacgcgga acccgccgcc gcaccgccgg catgaactgc cgccccggct
     6301 gcggcgcctg ctgcatcgcg ccgtcgatca gcagcccgat ccccggcatg cccgtcggca
     6361 agccggccgg cgtgcgctgc gtgcagctcg atgaccagca acgctgccgg ctgttcggcc
     6421 ggccggagcg cccggccgtg tgcgcctcgc tgcgcccgga gccggcgatg tgcggtgcgg
     6481 accgcgcgca ggcgctgttg cggctggcgc ggctggaggc gctgacacgg cccctggaca
     6541 gtcagggtcc gtgatggcca gcgccgggtt catgatcaga agccttgcac gctcgatgcc
     6601 gcagcgcttt cacccggcac cgccct
//
LOCUS       DAOQIE010000227         6616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6209_length_6616_cov_8.089316, whole genome shotgun sequence.
ACCESSION   DAOQIE010000227 DAOQIE010000000
VERSION     DAOQIE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_6616_cov_8.089316"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1982)
                     /gene="pepN"
                     /locus_tag="PK144_13380"
     CDS             complement(<1..1982)
                     /gene="pepN"
                     /locus_tag="PK144_13380"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNF66765.1"
                     /translation="MSDPKTIRLADYRAPAYLVDSVDLRFELGETGTDVQATLQLRRN
                     PAAGAGPAPLVLDGQQLELLALALDGVPLPATRYQLDDAQLTVTDVPAAFRLDLTTRI
                     HPETNTALEGLYRSSGNFCTQCEAEGFRKITYYPDRPDVMARFTTTVVAERARYPVLL
                     SNGNPVGRGELGDGRHWVRWVDPYPKPAYLFALVAGDLGCVRDSFVTASGRGVALEIY
                     TEHDKLDQCEHAMASLKRAMRWDEVRFGLEYDLDIYMIVAVSDFNMGAMENKGLNVFN
                     TKYVLARPDTATDTDFIGVESVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEF
                     TADMHSAAVARIDDVRTLRAVQFPQDAGPLAHPVRPESYIEINNFYTVTVYEKGAEIV
                     RMYQTLLGREGFRRGMDLYFRRHDGQAVSCEDFCAAMADASGRDLTQFLRWYRQAGTP
                     VLTASGRYDAEARCYTLRLSQHCPPTPESATKLPFHIPVAIGLLAPDGRALPLTLAGE
                     PQAGPPERVLELTGATQEFVFTGLDAAPVPSLLRGFSAPVKLDHAPAEDELLFLAAHD
                     TDAFNRWEAAQQLGVRAVLGLLDERAAGRAWTLPAGLSTLFGRVLADESLDPALAEQM
                     LTLPGEASLGEQRTPIDPGGLFEAREFLRRALAGAHR"
     gene            complement(2043..2948)
                     /locus_tag="PK144_13385"
     CDS             complement(2043..2948)
                     /locus_tag="PK144_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007113493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF66766.1"
                     /translation="MNYQITHRTTYRYYGRVSLCHNEARLLPRETSRQFVEESALEVL
                     PVPALRRERTDFFGNRVVNFSVQDIHQTLEVTARSRVTVAPRPSLTDTPSPGWEEVVC
                     LVRDLPELGAMEARQYTLESPFIRPNAALAAYARPSFTAGRPLLEAVLELNRRIFKEF
                     TYDPQFSTLATPLSQVLEERRGVCQDFAHLAIGCLRSLGLPARYVSGYLETLPPPGQP
                     RLVGADASHAWLSVFIPGLGWIDLDPTNDLLPDERHVTLAWGRDYADVAPLKGVMIGG
                     GSHALEVSVDVMPLLPPAEADDEAA"
     gene            complement(2973..5486)
                     /locus_tag="PK144_13390"
     CDS             complement(2973..5486)
                     /locus_tag="PK144_13390"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNF66767.1"
                     /translation="MIEPTSFPPAVHDLLSDYAPGSGWDELLDADGKVRPAWEYLLAQ
                     LDALGLGELQARHEETQRLLRENGVTFNAHEDGRAASRAWTLDPIPLPILGDEWAQLE
                     AGLAQRSRLLNRLAADLYGARTVFRRGVLPPELVHAHPGFLHAAWGALPAGARWLVFH
                     AIDFARDEQGRWLVLGDRAQAPSGAGYALENRITLARALPTVYREAPLKRLASFLETE
                     RVTLARLAPRHVDKPNVVLLTPGPASQGYFEHAYLANYLSLSLVQGEDLVVRDGKVSL
                     KTLGGLQPIDVILRRVTDGWCDPLELRGDSMLGVPGLLAAVREGGVVLANALGIGAIE
                     NPGLVPFLPALCRELLGEELLLQPVKSYWCGDGESLEAVLDRLDSLVIHQLTEQTITD
                     GLHLDAAARATLAARIRSAPHLYVAQEHVRPAHAPVWDGRKLVAQPVMMRGFCVAEED
                     GYRVMPGALGAVSPDGRMAVQGGTTKDVWVLAESAQPHVSLLRQAHGPIVVTRDGADL
                     PSRVADNLFWLGRYGERYDSRARLLREALLRLLERGEGAGEDGCLEDLLIALGIELGS
                     VDAGPRGRYLALRRAFLGVFDEQNDGGLREIFAGMLRNGRAVRDHLGDESFRVLNRLR
                     ESLRDLPSPPSASAGRKALEEHLTLLTAFFGLCNETMPHHYGWRFLDIGRFVERVLST
                     LTMLELVFVKTEHPGVPLWEVMLATTDNLTAYRRRYRSALHPSAILDLLLFDEDNPRS
                     VGYLLRRLRRQIERLPHPKATPYRSAEERLILEAQTTLALADIATLAEVTADGDPKGQ
                     RALGKLLRRLRAPLAELSEALSNSHFSHSEKPRQLIEMI"
     gene            complement(5618..>6616)
                     /locus_tag="PK144_13395"
     CDS             complement(5618..>6616)
                     /locus_tag="PK144_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF66768.1"
                     /translation="EARNDSLYELEIAFREMPTGEVPQPWLVDRLLRNLLVDLTGNTH
                     RSEFCIDKLYSPDSATGRLGLVEFRAFEMPPHARMSLLQQLLLRSLVARFWEDPYQGR
                     LCRWGTSLHDRWMLPHFLVEDLKDVLADLDRHGLRLDPEWFAPFSEFRFPVYGRIVQQ
                     DIQLELRQAVEPWHVLGEEAVMGGTARYVDSSVERMQVKVEGLNGDRYVLACNGRRVP
                     LAATGRQGEFVAGVRYRAWQPPSALHPTIPVHAPLVFDLVDTWNQRSIGGCTYHVSHP
                     AGRNFVVFPVNANEAEARRLARFWAYGHTPNAMQAPAEERSLDLPMTLDLRRRPGC"
BASE COUNT          973 a         2272 c         2435 g          936 t
ORIGIN      
        1 cgatgcgcgc cggccagcgc gcggcgcagg aattcacgcg cctcgaacag gccgcccggg
       61 tcgatcggcg tgcgctgctc gccgagggag gcctcgcccg gcagcgtcag catctgctcg
      121 gccagcgccg ggtcgaggct ttcatcggcg agcacgcgcc cgaacagcgt cgacagcccc
      181 gccggcagcg tccaggcccg gccggccgcg cgctcgtcga gcaggccgag caccgcgcgc
      241 acgccgagct gctgcgcggc ctcccagcgg ttgaaggcat cggtgtcgtg cgcggcgagg
      301 aacagcagtt cgtcctcggc cggtgcgtga tcgagcttca ccggggccga gaagccgcgc
      361 agcagcgagg gcaccggcgc ggcgtcgagc ccggtgaaca cgaactcctg cgtggctccg
      421 gtcagctcca gcacccgctc cggcggcccg gcctgcggct cgccggccag cgtcagcggc
      481 agcgcccggc catccggggc cagcaggccg atcgcgaccg ggatgtggaa cggcagcttg
      541 gtcgcggact ccggcgtggg cgggcaatgc tggctcaggc gcagcgtgta gcagcgcgct
      601 tcggcgtcgt agcggccgct cgccgtgagc accggcgtgc cggcctggcg gtaccagcgc
      661 aggaactgcg tcaggtcgcg accgctggca tcggccatcg ccgcgcagaa gtcctcgcag
      721 ctcacggcct ggccgtcgtg acggcggaag tacaggtcca tgccgcggcg gaagccctcg
      781 cgcccgagca gggtctggta catgcgcacg atctcggcgc ccttctcgta gaccgtgacc
      841 gtgtagaagt tgttgatctc gatgtaggac tccggccgca ccgggtgcgc gagcggtccg
      901 gcgtcctgcg ggaactgcac ggcgcgcagc gtgcgcacgt catcgatgcg cgcgaccgcc
      961 gccgagtgca tgtcggccgt gaactcctga tcgcggaaca ccgtcaggcc ttccttcagc
     1021 gacagctgga accagtcgcg gcaggtgacg cggttgccgg tccagttgtg gaagtactcg
     1081 tggccgatca ccgactcgac accgatgaag tcggtgtcgg tggccgtgtc cggccgggcc
     1141 agcacgtact tggtgttgaa gacgttgagg cccttgttct ccatcgcgcc catgttgaag
     1201 tccgacaccg cgacgatcat gtagatgtcg aggtcgtact ccagcccgaa gcgcacctcg
     1261 tcccagcgca tcgcgcgctt gagcgaggcc atcgcgtgtt cgcactgatc gagcttgtcg
     1321 tgctcggtgt agatctcgag cgcgacgccg cggcccgagg cggtcacgaa gctgtcgcgc
     1381 acgcagccga ggtcgccggc gacgagcgcg aacagatagg ccggcttcgg atacggatcg
     1441 acccagcgca cccagtggcg gccgtcgccg agctcgccgc gcccgaccgg gttgccgttg
     1501 gacagcagca ccggatagcg cgcgcgctcg gcgacgaccg tggtcgtgaa gcgcgccatg
     1561 acgtccgggc gatccgggta atacgtgatc ttgcgaaagc cctcggcctc gcactgcgtg
     1621 cagaagttgc cggaggaccg gtacaggcct tcgagcgcgg tgttggtctc ggggtggatg
     1681 cgcgtcgtga ggtcgagccg gaatgccgcc ggcacgtcgg tcacggtcag ctgcgcatcg
     1741 tcgagctgat agcgcgtcgc cggcagcggc acgccgtcga gcgcgagcgc gagcagttcg
     1801 agctgctggc cgtcgagcac cagcggggcc gggccggccc cggcggcggg gttgcggcgc
     1861 agctgcagcg tggcctgcac gtcggtgccg gtctccccga gctcgaagcg caggtcgacg
     1921 gaatccacca gataggccgg ggcgcggtag tcggcgagac ggatggtctt gggatcgctc
     1981 atcggcgggc tccggttcgg ctggaaccgc cgatggtaca cccgctccgc cagagcagga
     2041 cttcaggccg cctcgtcgtc ggcctccgcc ggcggcagca gcggcatcac gtcgaccgac
     2101 acttcgagcg cgtgcgaacc gccgccgatc atgacgccct tcagcggcgc gacatcggcg
     2161 tagtcgcgcc cccaggcgag cgtcacgtgg cgttcgtcgg gcagcaggtc attggtcgga
     2221 tcgagatcga tccagccgag gccggggatg aacaccgaga gccaggcgtg cgaggcgtcg
     2281 gccccgacca gccgcggctg gcccggcggc ggcagggtct ccagatagcc gctgacgtag
     2341 cgcgccggca ggccgagcga gcgcaggcag ccgatggcca gatgcgcgaa gtcctgacag
     2401 acgccgcggc gctcctccag cacctgcgac agcggcgtgg ccagcgtcga gaactgcggg
     2461 tcgtaggtga attccttgaa gatgcgccgg ttcagttcga gcacggcctc cagcaagggc
     2521 cggccggccg tgaacgacgg ccgcgcatag gcggcgagcg cggcgttcgg gcggatgaac
     2581 ggcgattcga gcgtgtactg gcgcgcttcc atcgcgccga gctcgggcag gtcgcgcacc
     2641 aggcagacga cctcctccca gcccggcgac ggcgtgtcgg tcagcgacgg gcgcggggcg
     2701 accgtcacgc gcgagcgcgc cgtgacctcc agcgtctggt ggatgtcctg caccgagaag
     2761 ttgacgacgc ggttgccgaa gaagtcggtg cgctcgcgcc gcagtgccgg caccggcagg
     2821 acctccagcg ccgattcctc gacgaactgg cggctggtct cgcgcggcag cagccgggct
     2881 tcgttgtggc acagcgacac gcgcccgtag tagcggtagg tcgtgcggtg cgtgatctga
     2941 tagttcatcg ctgggcttcc gtggcgcggc cgtcagatca tctcgatcag ctggcgcggc
     3001 ttctcggaat ggctgaagtg gctgttggaa agcgcctcgg acagctcggc gagcggagcg
     3061 cgcagccggc gcagcagctt gccgagcgcg cgctggccct tcggatcgcc gtcggcggtg
     3121 acctcggcca gcgtcgcgat gtcggcgagc gcgagcgtgg tctgggcttc gaggatcagc
     3181 cgctcctcgg cgctgcggta cggcgtggcc ttcgggtgcg gcaggcgctc gatctggcgg
     3241 cgcagccggc gcagcagata gccgaccgag cgcgggttgt cctcgtcgaa cagcagcagg
     3301 tcgaggatcg cgctcgggtg cagcgccgag cggtagcggc ggcggtaggc ggtcaggttg
     3361 tcggtggtcg cgagcatgac ctcccacagc ggcacgcccg ggtgctcggt cttcacgaac
     3421 accagttcga gcatcgtcag cgtgctcagc acgcgctcga cgaagcggcc gatgtcgagg
     3481 aagcgccagc cgtagtggtg cggcatggtc tcgttgcaga ggccgaagaa ggccgtcagc
     3541 agcgtcaggt gttcctccag cgccttgcgc ccggcgctcg cgctcggcgg actcggcagg
     3601 tcgcgcaggc tctcgcgcag gcggttcagc acgcggaagg attcgtcgcc gaggtgatcg
     3661 cgcacggcgc ggccgttgcg cagcatgccg gcgaagatct cgcgcagccc gccgtcgttc
     3721 tgctcgtcga acacgccgag gaaggcccgg cgcagcgcca gatagcgccc gcgcggaccg
     3781 gcgtcgacgc tgccgagctc gatgccgagc gcgatcagca ggtcttcgag gcagccgtcc
     3841 tcgccggcgc cctcgccgcg ttcgagcagg cgcagcagcg cctcgcgcag cagccgggcg
     3901 cggctgtcgt agcgctcgcc gtagcgcccg agccagaaca ggttgtcggc cacccggctc
     3961 ggcaggtcgg cgccgtcgcg cgtgaccacg atcgggccgt gcgcctggcg cagcaggctc
     4021 acgtgcggct gcgccgattc ggcgagcacc cagacatcct tggtcgtgcc gccctgcacg
     4081 gccatgcgcc cgtcggggct gaccgcgccg agggcgcccg gcatcacgcg gtagccgtct
     4141 tcctcggcca cgcagaagcc gcgcatcatc accggctgcg cgaccagctt gcgcccgtcc
     4201 cagaccggcg catgcgccgg gcgcacgtgc tcctgggcga cgtacaggtg cggcgcgctg
     4261 cggatgcgcg cggccagcgt ggcgcgggcg gcggcatcga ggtgcaggcc gtcggtgatc
     4321 gtctgttcgg tgagctggtg gatgaccagg ctgtcgagcc ggtcgagcac ggcttcgagg
     4381 ctttcgccgt ccccgcacca gtagctcttg accggctgca gcagcagttc ctcgccgagc
     4441 agctcgcgac agagcgccgg caggaagggt acgagtccgg ggttttcgat cgcgccgatg
     4501 ccgagcgcgt tggccagcac cacgccgccc tcgcgcaccg ccgccagcag ccccggcacg
     4561 ccgagcatcg aatcgccgcg cagttcgagc ggatcgcacc agccgtcggt gacgcggcgc
     4621 aggatcacgt cgatcggctg caggccgccg agcgtcttca gcgagacctt gccgtcgcgc
     4681 acgaccagat cctcgccctg caccagcgac agcgacaggt agttggcgag atacgcgtgc
     4741 tcgaagtagc cctggctcgc cggtccgggc gtcagcagca cgacgttggg cttgtcgacg
     4801 tggcgcggcg ccagacgcgc gagcgtcacg cgttcggttt cgagaaagct cgcgaggcgc
     4861 ttcagcggcg cctcgcggta gacggtcggc agcgcccgcg cgagcgtgat gcggttttcc
     4921 agcgcatagc cggcgccgga cggtgcctgg gcgcgatcgc cgagcaccag ccagcggccc
     4981 tgctcgtcgc gcgcgaagtc gatggcgtgg aacaccagcc agcgcgcccc ggccggcagc
     5041 gccccccagg ccgcgtgcag gaaacccgga tgggcatgca cgagttcggg cggcagcacg
     5101 ccgcggcgaa acaccgtgcg tgcgccgtag agatcggcgg cgaggcggtt cagcaggcgg
     5161 ctgcgctgcg cgagccccgc ctccagctgc gcccattcgt cgccgaggat cggcagcggg
     5221 atcgggtcga gcgtccacgc gcggctcgcg gcgcggccgt cctcgtgggc gttgaacgtg
     5281 acgccgttct cgcgcagcag ccgctgggtt tcctcgtggc gggcctgaag ctccccgagg
     5341 ccgagcgcat cgagctgggc gagcaggtat tcccaggccg gacgcacctt gccgtcggca
     5401 tcgagcagct cgtcccagcc gctgcccggc gcgtagtcgc tcagcaggtc gtggaccgcc
     5461 ggcgggaaac tggtcggctc gatcatggcc tgcgcgtacc gggtgactga aacggccgtc
     5521 tatcgtagcg cgccacgacc gcggacggcg ccccctaccc ccgatgttcc gggagccggg
     5581 tgcgccgccg cggccggcgc gtcgtggtcc agccgcctca gcagcccgga cgacggcgca
     5641 ggtcgagcgt catcggcagg tcgaggctgc gttcctcggc cggcgcctgc atcgcgttcg
     5701 gcgtgtggcc gtaggcccag aagcgggcca gacggcgcgc ctcggcttca ttggcgttga
     5761 ccgggaacac gacgaagttg cgaccggccg ggtgcgagac gtgatacgtg cagccaccga
     5821 tcgagcgctg gttccaggta tcgacgaggt cgaagaccag cggcgcgtgc accgggatgg
     5881 tcggatgcag cgcggacggc ggctgccagg cgcgatagcg cacgccggcg acgaactcgc
     5941 cctgacgacc ggtggccgcg agcggcacgc gccggccgtt gcaggcgagc acgtagcgat
     6001 ccccgttgag gccttcgacc ttgacctgca tgcgctcgac cgaggaatcg acgtagcgcg
     6061 cggtaccgcc catgacggct tcctcgccga gcacgtgcca gggctcgacg gcctgacgca
     6121 gttcgagctg gatgtcctgc tgcacgatgc gcccgtagac cgggaagcgg aactccgaga
     6181 acggcgcgaa ccattccgga tcgaggcgca ggccgtgacg gtcgaggtcg gcgagcacgt
     6241 ccttgaggtc ctcgaccagg aagtgcggca gcatccagcg atcgtgcagg ctggtgcccc
     6301 agcggcacag gcggccctga tatggatctt cccagaagcg cgcgaccagc gagcgcagca
     6361 gcagctgctg cagcaggctc atgcgcgcgt gcggcggcat ctcgaaggcg cggaactcga
     6421 ccagaccgag gcggccggtc gccgaatccg gcgagtacag cttgtcgatg cagaactcgg
     6481 agcggtgcgt gttgccggtg aggtccacca gcaggttgcg cagcagccgg tcgaccagcc
     6541 acggctgcgg cacctcgccg gtcggcatct cgcggaacgc gatctccagt tcatacaggc
     6601 tgtcgttgcg cgcctc
//
LOCUS       DAOQIE010000228         6580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6269_length_6580_cov_7.870038, whole genome shotgun sequence.
ACCESSION   DAOQIE010000228 DAOQIE010000000
VERSION     DAOQIE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6269_length_6580_cov_7.870038"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PK144_13400"
     CDS             <1..468
                     /locus_tag="PK144_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66769.1"
                     /translation="ARVQLEGVSEPLAQWTLLANLPPALAPATLARWYYWRWRIESYF
                     KLLKSAGHHVEQWQQESAPAIARRLLIAAAACVTVWRLSLREDARAAEARAFLARLSG
                     RQIKPGKPPAASVLLAGLYRLLTALMLLEHYEPEQLLRLARDALGRLQGGEDV"
     gene            578..1228
                     /locus_tag="PK144_13405"
     CDS             578..1228
                     /locus_tag="PK144_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66770.1"
                     /translation="MNDALLPPGGTTLPDLVHELSQPLATIASYAEACHLLLTKPGSE
                     PLMAATLTAIVAQASLAGQLLQALRQALHGAGTPAPPAGDDTPTLAALLARLAPVLAT
                     HAETQAMTPAVGQWRTAAVVAAGLDAELPTLAARLHAVSDQPCHLLAALAPEGGARLY
                     LMPAPAAAASITGQHRLLDTDTADCAQRLLGCGISLSPHWQADRGLCFELRLPGPD"
     gene            complement(1273..5514)
                     /locus_tag="PK144_13410"
     CDS             complement(1273..5514)
                     /locus_tag="PK144_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNF66771.1"
                     /translation="MVGLGASAGGVEAFSEFLSAMPAASGMAFVFVLHLDPERSSVLT
                     EILARHTTMPVTEVTHPVQVVPDHVYVIAPNCCLEYQNGRLVPREPETPRWLRKPIDV
                     LLRSLARSCGARAIGVVFSGGGSDGTLGLKDIRNAGGLVLVQTPETAMQDSMPTSAID
                     AGLDDHVLAISAMPAVLIEHVQHRWHSEPVPPEPEPAEAEPEPEPEPEDADREKPDLE
                     DVLALLRARQDFDFRCYKKGMLARRVQRRMSALRLRESKDYLALLQRDSAEFDVLAAD
                     LMISVTQFFRNPQAWDHLAEQVLPELCELASAERPLRAWVPACASGEEVYSLAILLLE
                     RLLPGQPHIQLFASDSSEAALNVARTGLYPDSITAHVTPARLARFFRAEGTRYRVIRE
                     VRDCVTFAVQNLINDPPFGRIDLISCRNFMIYLEPEMQERMLALFHFALRPGGYLFLG
                     NVESIGGQFDLFEPVSHKWRVYRRIGTPRLARDGYPPTLRRPSGVQNWAVRLDAPRKA
                     GQIPAAMAAPELSGAGPLLGAKVSGIAGYAQQIVLERYTRACVLVNRRYEILYFFGPT
                     HEFLRQPVGEHTADLFAWTRDGLRSMLRSALHEISTTAANRVVRQHAAEAAGSGDIEC
                     IVEALRVPRELDGLLLIAFTRLPVVGPPPREVAGTADASGTAIAQLEFELARAREDLS
                     GMTEQLESAYEEFTASNEEVMSINEELQSANEELETSKEELQSLNEELVTSNQQLETK
                     NQQLEEINADLRNLLASTQIATLFLDEHFCIRRYTPAMAQLMRVIPSDIGRSVEDISC
                     DFDRVALLAQSVRVLEHLAPIETEIRTYDGRWHLCRVLPYRTEDNRIGGVVVTFTDVS
                     VRHRLEVRERLMLALNDRLAELQDPADIVTAVSELIERHLGAAWSRYAPVDSAQQWLY
                     VEGSSVGARVSLTRLPADVLRTLRRGQPLISTGNAGADPGLHAALTTLGAPQQLPGTV
                     LLFAPVVDGRLLTLVGVGHTGVYLWTDEDLDLVSVLAERTWIAIEKIEVNRTLVRRAT
                     QLAATDRLKNEFLATLGHELRNPLVPIRSTLDLLLGADQEGQLQAQLQLVDRQVQQLQ
                     RLVDDLLDASRIEQIGQIRLRMQDLDLDDVLQMAIEGQQPRFVARRQDFSYLPPSSPL
                     ALVGDRVRLVQAVGNLLHNASRYSPAGARIELMAERSGSEAVIRVRDNGRGIAPERLA
                     QLFRLFVHSAPDDPGDSPSEPGSGDDSLSFGLGLVLARRLIELHGGTLTAASPGPGCG
                     STFTIRLPLDAPADVRPLIAGTAVLPEPLPVVPRRVLLVDDNNDVRTALAALLEAMGH
                     EVCALSDGHEVSDAVGSFGPHTVLLDLAMPKMNGYAVARALRERYPDAALQLVALTGF
                     GGPDEQQRIREAGFDRHLLKPVSREQLAALLAGGG"
     gene            5790..6470
                     /locus_tag="PK144_13415"
     CDS             5790..6470
                     /locus_tag="PK144_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66772.1"
                     /translation="MILGTRASVCARSSAAVQDEPPRRFFMLFPLMTATCKAIVQTGP
                     AREQRRHAGSKPGGGRKVCHVRTSARPRCPLSGTLQGFLDSLCPLLPAATDYAILGFD
                     PHLHCCYASGSALGTLGIENPLRGTELQALAPALRTVVSGLCRCVFAGALTETGITLG
                     PQRLRLTGMPLLDSSGAISMALIRILPVDPLPDDRTSAVEDVRNRSPDGTADWHDPER
                     RRSTPAGR"
BASE COUNT          976 a         2346 c         2301 g          957 t
ORIGIN      
        1 gcgcgcgtgc agcttgaggg cgtcagcgag ccgctggcgc agtggacgct gctggccaac
       61 ttgcccccgg cgctcgcgcc cgcgacgctg gcccgctggt actactggcg ctggcgcatc
      121 gagtcgtact tcaagctgct caagagcgcc ggccaccacg tcgagcagtg gcagcaggaa
      181 agcgccccgg ccattgcccg gcgtttgctg atcgccgctg ccgcctgcgt gacggtctgg
      241 cgcctgagcc tgcgcgaaga cgcccgcgcc gccgaggccc gggcgttcct ggcacgcctc
      301 agcggccgtc agatcaagcc cggaaaacca ccggcggcca gcgtcttgct cgccggactc
      361 taccgtttgc tcaccgccct gatgctgctc gaacattacg aaccagagca gttgctgcgt
      421 ctggccaggg atgcactggg cagactgcag gggggcgaag atgtgtagat accaatgccc
      481 cgcgagggac ggcaaggacg gcgtgttccg ccgggatcgg gatgaatgcc catgagcgac
      541 cggggccacg acccggcacc acccgcaccg acgacgcatg aacgacgccc tcctgccgcc
      601 cggtggcacg acgctgcccg atctggtcca cgaactgagc cagccgctcg ccacgatcgc
      661 gagctacgcc gaagcctgtc atctgctgct gacgaagccc ggcagcgaac ccctgatggc
      721 cgcgaccctg acggcgatcg tggcgcaggc cagcctggcc gggcaattgc tgcaggcgct
      781 gcggcaggcg ctgcacggcg ccggcacgcc ggccccgccg gccggggatg acacgccgac
      841 gctcgccgcc ctgctggcac ggctcgcgcc ggtgctggcc acccacgccg aaacgcaggc
      901 catgacgccc gccgtcggcc agtggcgcac ggccgcggtg gtcgcggccg gtctcgatgc
      961 cgaactgccg acgctggctg cccgcctgca cgccgtcagc gatcagccct gccatctgct
     1021 cgcggcactg gcgcccgaag gcggcgcccg gctgtacctg atgcctgcgc cggccgcggc
     1081 ggcgtcgatc accggccagc accggctgct cgacaccgac acggccgact gcgcgcagcg
     1141 actgctgggc tgcggcatca gcctgagccc gcactggcag gccgaccgcg gcctgtgctt
     1201 cgaactgcgc ctgccgggcc cggactgaac gcaccgggcc gccggagcgc cacgccccgc
     1261 aagcggcccg gctcagcccc ctccggcgag cagggccgcg agctgttcgc ggctcacggg
     1321 cttcagcagg tggcggtcga acccggcctc gcggatgcgc tgctgctcgt ccgggccgcc
     1381 gaagccggtc agcgcgacca gttgcagcgc ggcgtccgga taccgctcgc gcagcgcgcg
     1441 cgccacggcg tagccgttca tcttcggcat cgccagatcg agcagcaccg tgtgcggccc
     1501 gaagcttccg acggcgtccg acacctcgtg cccgtccgag agcgcgcaga cctcgtgccc
     1561 catcgcttcg agcagcgccg cgagggcggt gcgcacgtca ttgttgtcat cgacgagcag
     1621 cacgcgccgc ggcacgaccg gcagcggctc cggcagcact gccgtgccgg cgatgagggg
     1681 ccggacatcg gccggggcat cgagcggcag gcggatcgtg aacgtgctgc cgcagccggg
     1741 gccgggactc gccgcggtca gcgtcccgcc gtgcagctcg atgagccggc gcgcgagcac
     1801 gagtccgagg ccgaagctga ggctgtcgtc gccggagccg ggctcggagg ggctgtcgcc
     1861 gggatcgtcc ggggcgctgt gcacgaacag ccggaacagc tgcgccagcc gctcgggggc
     1921 gatgccgcgg ccgttgtcgc gcacgcggat cacggcctcg ctgccgctgc gctcggccat
     1981 cagctcgatg cgggccccgg ccgggctgta gcggctggcg ttgtgcagca ggttgccgac
     2041 cgcctgcacc aggcgcaccc ggtcgccgac gagcgcgagc gggctggacg gcggcaggta
     2101 ggagaagtcc tggcggcgcg cgacgaagcg cggctgctgg ccctcgatcg ccatctgcag
     2161 cacgtcgtcg agatcaagat cctgcatgcg caggcggatc tggccgatct gctcgatgcg
     2221 gctggcatcg agcaggtcat cgacgaggcg ctgcagctgc tgcacctggc ggtcgacgag
     2281 ctgcagctgc gcctgcagct gcccttcctg atcggcgccg agcagcaggt cgagggtgct
     2341 gcggatcggc acgagggggt tgcgcagttc gtggccgagg gtcgcgagga actcgttctt
     2401 gagccggtcg gtggccgcga gctgcgtggc gcggcgcacg agcgtgcggt tgacctcgat
     2461 cttctcgatg gcgatccacg tgcgctcggc gagcacgctg acgaggtcga gatcctcgtc
     2521 ggtccacaga tagacgccgg tgtggccgac gccgaccagc gtcagcagcc ggccgtcgac
     2581 gaccggcgcg aacagcagca ccgtgccggg cagctgctgc ggggcgccga gcgtcgtcag
     2641 cgccgcgtgc aggcccggat cggcgccggc attgccggtg ctgatcagcg gctggccgcg
     2701 gcgcagcgtg cgcagcacgt cggccggcag cctcgtcagc gacacgcgcg ccccgacgct
     2761 ggagccctcg acgtacagcc actgctgcgc gctgtcgacc ggggcgtagc ggctccaggc
     2821 cgcgccgaga tggcgctcga tcagctcgga aacggccgtg acgatgtcgg ccgggtcctg
     2881 gagttcggcg aggcggtcgt tgagggcgag catcagccgt tcgcgcactt ccaggcgatg
     2941 gcgcacgctc acgtcggtga aggtgacgac gacgccgccg atgcggttgt cctcggtgcg
     3001 gtacggcagc acgcggcaca gatgccagcg cccgtcatag gtgcggatct cggtttcgat
     3061 cggtgcgagg tgttcgagca cgcgcacgga ctgcgcgagc agcgccacgc ggtcgaagtc
     3121 gcagctgatg tcctcgaccg agcggccgat gtcgctcggg atcacgcgca tcagctgcgc
     3181 catcgccggc gtgtagcggc ggatgcagaa gtgctcgtcg aggaacagcg tcgcgatctg
     3241 cgtgctcgcg agcaggttgc gcagatcggc gttgatctcc tccagctgct ggttcttggt
     3301 ttcgagctgc tggttgctgg tgaccagttc ctcgttcagc gactgcagct cctccttcga
     3361 ggtttcgagc tcctcgttgg ccgactgcag ctcctcgttg atcgacatca cctcctcgtt
     3421 gctggccgtg aactcctcgt aggcgctttc gagctgctcg gtcatgccgg agaggtcctc
     3481 gcgcgcgcgc gccagctcga attcgagctg cgcgatcgcg gtgccgctgg catcggccgt
     3541 gccggcgacc tcgcgcggcg gcgggccgac gacgggcagg cgcgtgaagg cgatgagcag
     3601 caggccgtcg agctcgcgcg gcacgcgcag ggcctcgacg atgcactcga tgtcgccgct
     3661 gccggcggcc tcggcggcgt gctggcgcac cacgcggttg gccgccgtcg tgctgatctc
     3721 gtgcagcgcg gagcgcaaca tgctgcgcag gccgtcgcgc gtccacgcga acaggtcggc
     3781 ggtgtgctcg ccgaccggct ggcgcaggaa ctcgtgcgtc gggccgaaga agtacaggat
     3841 ctcgtagcgg cggttgacga gcacgcaggc gcgcgtgtag cgctccagca cgatctgctg
     3901 cgcgtagccg gcgatccccg agaccttggc gccgagcagc ggccccgcac cgctcagctc
     3961 cggtgccgcc atcgccgccg gtatctgccc cgccttgcgc ggcgcatcga ggcgcacggc
     4021 ccagttctgc acgcccgagg ggcggcgcag cgtcggcggg tagccgtcgc gcgccagccg
     4081 cggagtgccg atgcgccggt acacgcgcca cttgtgcgag accggctcga acaggtcgaa
     4141 ctgcccgccg atcgactcga cgttgccgag gaacaggtag ccccccggac gcagcgcgaa
     4201 gtgaaacagc gccagcatgc gctcctgcat ctccggttcg agatagatca tgaagttgcg
     4261 gcagctgatc aggtcgatgc ggccgaacgg tggatcgttg atgaggttct gcaccgcgaa
     4321 ggtcacgcag tcgcgcacct cgcggatcac ccggtagcgc gtgccctcgg cgcggaagaa
     4381 gcgcgccagc cgcgccggcg tcacgtgcgc cgtgatgctg tccggataga ggccggtgcg
     4441 cgcgacgttg agcgcggcct cgctcgaatc cgaagcgaac agctggatgt gcggctgccc
     4501 cggcagcagg cgctccagca gcaggatcgc gaggctgtag acctcctcgc cgctggcgca
     4561 ggccggcacc caggcccgca gcggccgctc ggccgaggcc agctcgcaga gctcgggcag
     4621 cacctgctcg gccagatggt cccaggcctg cgggttgcgg aagaactgcg tgacgctgat
     4681 catcagatcg gcggccagca catcgaactc cgcggagtca cgctgcagca gcgcgagata
     4741 gtccttgctc tcgcgcaggc gcagcgcgct catgcgccgc tggacgcgcc gcgccagcat
     4801 gcctttcttg tagcagcgga agtcgaagtc ctggcgcgcg cgcagcagcg ccagcacatc
     4861 ctccagatcc ggcttctccc gatccgcatc ctccggctcc ggctccggct ccggctccgc
     4921 ttcggccggt tccggctccg gcggcacggg ctcgctgtgc cagcgatgct gcacatgctc
     4981 gatcagcacc gccggcatgg cggagatcgc cagcacatga tcgtcgagac cggcgtcgat
     5041 cgcgctggtc ggcatgctgt cctgcatcgc ggtctcgggc gtctgcacca gcacgagccc
     5101 gcccgcgttg cggatgtcct tgaggccgag cgtgccgtcg ctgccgccgc ccgagaagac
     5161 cacgccgatc gcgcgcgcgc cgcagctgcg tgcgagcgag cgcagcagca cgtcgatggg
     5221 cttgcgcagc cagcgcgggg tttccggctc ccgcggcacg agccggccgt tctggtattc
     5281 gaggcagcag ttcggcgcga tcacgtagac gtgatcgggc accacctgca ccggatgcgt
     5341 gacctccgtg accggcatgg tcgtgtgccg cgccaggatt tcggtcagca cgctgctgcg
     5401 ctcgggatcg agatgcagca cgaacacgaa ggccatgccg ctggcggccg gcatcgcgct
     5461 caggaattcg ctgaacgcct cgacgccccc tgccgaagcg ccgaggccga ccaccggaaa
     5521 tgcacaccgg gccccgccgg ccggccctga tgcagctcgt ctggacatcg tcttcccccc
     5581 tctgcgccac gaatccgccg gcaagtttaa gtccgcttcg cccgtcctcc tcggcttctc
     5641 ccgacgaagt aggtacaaac cggtagccgc cgtggaaagc gccaatggcg atgcactgct
     5701 cctgacagca tagtgacagg cagccctgca tcccgagcca cggccaggat ggtggcaggc
     5761 agcgagacgg ggccaagaga agcgacagca tgatcctggg gacacgcgcc agcgtgtgcg
     5821 ccagaagcag cgcggcggtt caggacgagc ctccgcgccg ctttttcatg ctttttccat
     5881 taatgaccgc cacttgcaag gccatcgtac agacaggacc tgcccgggag cagcgacggc
     5941 atgccggatc gaaaccgggt ggagggagaa aagtctgcca tgtccgaacc tctgcccggc
     6001 ctcgatgccc cctgtcagga acacttcagg gctttctcga ttccctctgt ccgttgctgc
     6061 ccgcagcgac cgactacgcc atcctcggct tcgatcctca cctgcactgc tgctacgcca
     6121 gcggctctgc actcggcacg ctcggcatcg aaaatccgct ccggggcacg gagttgcagg
     6181 cgctcgcgcc ggcgctgcgc acggtcgtga gcgggctttg ccgctgcgtc ttcgccggcg
     6241 cgctgaccga aaccgggatc acgctcggcc cgcagcggct gcgcctgacc ggcatgccgc
     6301 tgctcgacag cagcggtgcg atcagcatgg ccctgatccg catcctgccg gtcgacccgc
     6361 ttcccgatga ccgcacgagc gcagtcgagg atgtccgcaa ccgcagcccc gacgggaccg
     6421 ccgactggca cgaccctgaa cggaggcgaa gcacgccggc aggccgatga acaccgggac
     6481 cacacctccg ccgcctccgg gcgtcggcat cgtcgatgat gaccgcgagc tgcgcacggc
     6541 cctgcgcctg ctgctcgaaa gcgccggcca tgtcgtcgag
//
LOCUS       DAOQIE010000229         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6271_length_6579_cov_8.597026, whole genome shotgun sequence.
ACCESSION   DAOQIE010000229 DAOQIE010000000
VERSION     DAOQIE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_6579_cov_8.597026"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..233
                     /locus_tag="PK144_13420"
     tRNA            157..233
                     /locus_tag="PK144_13420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Asp,seq:gtc)
     gene            408..2309
                     /locus_tag="PK144_13425"
     CDS             408..2309
                     /locus_tag="PK144_13425"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNF66773.1"
                     /translation="MLQTIRDRASGWFAYLVFGMLLIPFALWGINYYTHDGTAPFVAR
                     VNGEEIGLARYQQTLQNQRERLRQGGVQDVDENRLKHMIVGQLIDERLLEQFTRAEHM
                     RAGNVQVSAWVQSVEAFRAGDRFDPETYQAVLRRQGYTVTDFENMLRTSLATDQLRQG
                     LAATALLSTTDRSRLIELVAQKRDFEVLTLALAPVSARQSVDDAAIEARFRAQPQAYV
                     FPEQLRLEVLDLSPDLLLSRVGTPQDADLRGLYTQQQASLGRPETRTASHILVRVPDG
                     ASPAQVEQARQQALGYRETVVSGKESFEALGKSLAGTANVEVGDLGALERGVMDPAFD
                     QALFGLGKVGDLSEPVRTDFGFHVIRLDAVTPGSVPPFEEVRAQLVQLWQRQQAESAF
                     FDAADRLSTLVFENPDNLSTAARELGLQTRTSDWLTRSGSDDPLLNDARVLAAAFSDE
                     VLKDGRNSEPVQLDAQHVIVVRVAEHRDAKPKTLAEAREQIVAELKDSQARAELAKAA
                     EQLMQAVRDGTAPEAAAQAAGATRQAPGPLTRNAAVLTPELLRAAFRVPPPAGSQPAL
                     SVLELASGDRAVIVLKSVQPGTEADVAEADRQALTQRAALQSGERDFRGLLDALRAAA
                     KIENRADTL"
     gene            complement(2439..3254)
                     /locus_tag="PK144_13430"
     CDS             complement(2439..3254)
                     /locus_tag="PK144_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNF66774.1"
                     /translation="MGFLAGKRALIVGLASNRSIAYGIARAMHREGAELAFTYQNDKL
                     KPRVEKMAAEFGSTLTWPCDVSNDAEIDAVAEALAGAWPEGYDIVVHSVAFAPGEALD
                     GDFLDGISRENFRIAHDVSSYSFAALAKATRPKLRAGGALLTLSYLGAMRSIPNYNVM
                     GLAKASLEANVRYLAATLSPAQLDADGKFVAHGVRVNAISAGPIRTLAASGIAGFRKM
                     LEAFEAAAPTRQCVTIDEVGNVAAFLCSDLASGMTGEIVYVDGGFHNVAMANL"
     gene            3360..5150
                     /locus_tag="PK144_13435"
     CDS             3360..5150
                     /locus_tag="PK144_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNF66775.1"
                     /translation="MARFRLPAVAGTCLFPLLLSAPLHAGPAVALGYAPKYGPGFTHF
                     EYVNPDAPKGGTLTLDAIGGFDRLNPWLLKGTPAAGVADLMCDTLVEQSRDEPFSVYG
                     LLADDIALAPDRRSVRFHLRPEARFADGSPVRAADVKASFDTLTGKFAHPQYRLYWAD
                     VQAAVVEDAQSVRFEFARENPELHLIIGQMPVFAAKWIEGRQFDEIVREAPLCSGPYA
                     LEGFTAGRRIEYRRRPDYWAAELPVRRGQYNFDRVVFEYFQDQAVALEGFKAGVFDVA
                     QVNIAKQWVRDYTGPKFDSGELVKAELAHRNNAGMQAFAFNLRRPLFQDIRVRRALAL
                     AFDFEWSNQTLFEGLYRRSNSYFTNSDLAASGVPQGAELALLEPFRAQLPAELFERPW
                     QPPSTEAPGSLRDNLRAALALLKEAGWTLADNVLVKDGRRLEFEMLLVNRGFERVIAP
                     YAKNLERLGVRLNYRTVDTSLYQQRVDNFDFDALVQVWPQSPSPGNEQSGNWGSASAD
                     QPGSNNLLGLKDPVVDALLAKLVYAPDRAALVTAAHALDRVLLWGDYVVPNWYSPVHR
                     LAWRNRFAHPQTLPLYYQPIDWVISSWWAK"
     gene            5154..6212
                     /gene="yejB"
                     /locus_tag="PK144_13440"
     CDS             5154..6212
                     /gene="yejB"
                     /locus_tag="PK144_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HNF66776.1"
                     /translation="MAAYLARRVLLMVPTLLGILLVSFTIIQFVPGGPVEQLVNELTH
                     RGAAGEASSGGGSGVAYRGAQGLGPQEVEAIRRLYGFDKPAIERFADMVVRYARFDLG
                     DSYFHHRSVLGLIADKLPVSVSLGVWSFLILYAVSIPLGIRKALQDGSRFDVATSTLI
                     LVGYAIPGFVLGLLLLVLFGGGSFWQLFPLRGLTSEGWEQLPWYAQVLDYLWHLVLPI
                     TASTVGGFAVMTLLTKNSFLEEIRKQYVLTARAKGLTERAVLYRHVFRNAMIPLVTGF
                     PAAFIGMFFTGSLLIETTFSLDGLGLLSYQSVLRRDYPVVMGTLYLYTLLGLLAKLLS
                     DLSYVLVDPRIGFESVDR"
     gene            6209..>6579
                     /locus_tag="PK144_13445"
     CDS             6209..>6579
                     /locus_tag="PK144_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF66777.1"
                     /translation="MSAPAADPAPLAAGLSPRARAWRRFRANRRGWWSLWIFGVLFGL
                     SLGAELLSNDRPLLVHYEGAYYFPLLKTYPETAFGGDFDTEADYHDPYLRERLAGGDN
                     WLLDPPNPYRYDTIRLDGQRA"
BASE COUNT          962 a         2251 c         2299 g         1067 t
ORIGIN      
        1 aataccggcc tgtcacgccg ggggtcgcgg gttcgagccc cgtccgctcc gccaatcatt
       61 cctgaaattc atcgtccctg cggaagtcgc ggaccggatt cgtccgggcg actccggcac
      121 ggcgatgatt cggcgcaatg ccgatctgca aattccggag cggtagttca gttggtcaga
      181 ataccggcct gtcacgccgg gggtcgcggg ttcgagcccc gtccgctccg ccattccctg
      241 cctttcccgt ttccagtgcg tggcctcacc ggcgccgatc gtgcgccctg cggctggcca
      301 ttgcgttaga ctggagccac atccgttgcg gatgatgccg gcgaacaggg taactgcatg
      361 gagccggcgc atccgcgttc cgtctctccg tcacggaaat cctgttcatg ctgcagacga
      421 tccgggaccg tgcctcaggc tggttcgctt acctcgtgtt cggaatgctg ctcattccgt
      481 tcgcgctctg gggcatcaac tattacaccc atgacggaac cgcgccgttc gtcgcccggg
      541 tcaacggcga agagatcggg ctggcgcgtt accagcagac cctgcagaac cagcgtgagc
      601 ggctgcgtca gggcggcgtg caggatgtcg acgagaaccg gctcaagcac atgatcgtcg
      661 ggcaactgat cgacgagcgc ctgctggagc agttcacccg cgccgagcac atgcgggccg
      721 gcaacgtgca ggtctcggcc tgggtgcagt cggtcgaggc cttccgggcc ggtgatcgct
      781 tcgatcccga aacctatcag gccgtgctgc gccgccaggg ttatacggtc acggacttcg
      841 agaacatgct gcgcacctcg ctggccaccg atcagctgcg tcaggggctg gcagcgaccg
      901 cgctgctcag cacgaccgac cgcagccggc tgatcgaact ggtcgcgcag aagcgcgatt
      961 tcgaggtgct gacgctggcg ctggcgccgg tcagcgcgcg tcagagcgtc gatgacgcgg
     1021 ccatcgaggc ccgtttccgc gcgcagccgc aggcctatgt ctttcccgag cagttgcggc
     1081 tcgaagtgct ggatctgagc ccggatctgc tgctgtcgcg ggtcggcacg ccgcaggacg
     1141 ccgacctcag gggcctgtac acgcagcagc aggccagcct cggccggccg gaaacccgca
     1201 ccgcctcgca catcctcgtg cgcgtgccgg acggcgcgag tcccgcgcag gtcgagcagg
     1261 cgcgccagca ggcgctcggc taccgcgaga ccgtcgtcag cggcaaggaa agcttcgagg
     1321 cgctcggcaa gtccctggcc ggcacggcca acgtcgaggt cggcgatctc ggcgcgctcg
     1381 agcgcggcgt catggacccg gctttcgatc aggcgctgtt cggtctcggc aaggtcggcg
     1441 atctcagtga accggtgcgc accgatttcg gcttccacgt gatccggctc gatgcggtga
     1501 cgccgggcag cgtgccgccg ttcgaggagg tccgggcgca gctcgtgcag ctctggcagc
     1561 gccagcaggc cgagagcgca ttcttcgatg ctgccgaccg gctctcgacg ctggtcttcg
     1621 aaaaccccga caacctgagc accgctgccc gcgaactcgg cctgcagacg cgcaccagcg
     1681 actggctgac gcgcagcggc agtgacgatc cgctgctcaa cgatgcgcgc gtgctggcgg
     1741 cggcgttctc ggacgaggtg ctgaaggacg ggcgcaacag cgagccggtg cagctcgatg
     1801 cccagcacgt catcgtcgtg cgcgtcgccg agcatcgcga tgccaagccg aagacgctcg
     1861 ccgaagcgcg cgagcagatc gtcgcagagc tgaaggacag tcaggcgcgc gccgaactgg
     1921 cgaaggcggc cgagcagctg atgcaggccg tgcgcgatgg cacagcgccg gaggcggcgg
     1981 cgcaggcggc cggtgcgacg cgccaggctc ccggcccgct gacccgcaac gcggcggtgc
     2041 tgacgccgga actgctgcgc gcagccttcc gggtgccgcc gccggctggc agccagcccg
     2101 cgctcagcgt gctggagctg gccagcggcg accgggcggt gatcgtgctg aagtccgtgc
     2161 agcccgggac cgaggccgac gtggcggaag ccgaccggca ggcgctcacg cagcgggcgg
     2221 cgctgcagtc cggcgagcgc gacttccgcg gcctgctcga tgcgctgcgc gcggcggcga
     2281 agatcgagaa tcgcgccgac acgctctgat tgcgcgggcg accggcctgt ccggataccg
     2341 ggtcggtccg gatcaggacc ggcccggaca tgcgcgggcc cgctgccgtc gatccggcag
     2401 cgggcccgcg tcgtgtccgc cttgaggagc gggggcgctt acaggttcgc catcgcgacg
     2461 ttgtggaagc cgccgtcgac gtagacgatc tcgccggtca tgccggaggc caggtccgag
     2521 cacaggaagg ccgcgacgtt gccgacttca tcgatggtca cgcactggcg cgtcggcgcg
     2581 gcggcctcga aggcttcgag catcttgcgg aagccggcga tgccgctggc cgcaagcgtg
     2641 cggatcgggc cggccgagat ggcgttgacg cgcacgccgt gcgcgacgaa cttgccgtcg
     2701 gcgtcgagct gcgccggact cagcgttgcg gccagatagc ggacgttggc ttcgaggctg
     2761 gccttggcga ggcccatgac gttgtagttc gggatgctgc gcatcgcgcc gaggtagctg
     2821 agcgtcagca gcgcgccgcc ggcacgcagc ttcgggcgcg tggccttggc gagggccgcg
     2881 aagctgtagg aactcacgtc gtgggcgatg cggaagttct cgcgcgagat gccgtcgagg
     2941 aagtcgccat cgagcgcctc gccgggcgcg aacgccaccg aatgcacgac gatgtcgtag
     3001 ccctcgggcc aggcgccggc gagggcttcg gcgacggcgt cgatctcggc atcgttgctg
     3061 acatcgcagg gccaggtcag cgtgctgccg aactcggcgg ccatcttctc gacgcggggc
     3121 ttgagcttgt cgttctggta cgtgaacgcg agttcggcgc cttcacggtg catggcccgg
     3181 gcgatgccgt aggcgatcga gcggttgctg gcgagaccga cgatcagggc gcgcttgccg
     3241 gcgagaaaac ccatggagga ctcctgtggt catttgctat cgtcgaaagc cggccgttcc
     3301 cggctcccgg tcaatcctga aagcataacc tgcgggtttc cgccctcaaa ggatcgccga
     3361 tggcccgatt ccgcctgccg gccgtcgccg gcacctgcct gttcccgctg ctgctgtccg
     3421 cgccgcttca cgccgggcct gcggtcgcgc tcggctatgc cccgaagtac ggtcccggct
     3481 tcacgcattt cgagtacgtg aatcccgatg cgcccaaggg cggcacgctg acgctcgatg
     3541 cgatcggcgg cttcgaccgc ctgaacccgt ggctgctgaa gggcacgccg gcggccggcg
     3601 tggcggacct gatgtgcgac acgctggtcg agcagtcgcg cgacgaaccc ttcagcgtct
     3661 acggcctgct cgccgatgac atcgcgctcg cgccggacag gcgctcggtg cgcttccacc
     3721 tgcggcccga ggcgcgcttc gccgatggct cgccggtgcg cgcggccgac gtgaaggcga
     3781 gcttcgacac gctgaccggc aaattcgcgc atccgcagta ccggctgtac tgggccgacg
     3841 tgcaggccgc cgtcgtcgag gatgcgcaga gcgtgcgctt cgagttcgcg cgcgagaacc
     3901 ccgagctgca cctgatcatc ggccagatgc cggtgttcgc ggcgaagtgg atcgaggggc
     3961 gccagttcga cgagatcgtg cgcgaagccc cgctctgcag cgggccgtat gcgctggagg
     4021 gtttcacggc cgggcggcgc atcgaatacc ggcgccggcc cgactactgg gcggcggagc
     4081 tgccggtgcg ccgcggccag tacaacttcg atcgcgtggt gttcgagtac ttccaggatc
     4141 aggcggtcgc gctcgaaggc ttcaaggccg gcgtgttcga cgtggcccag gtcaacatcg
     4201 ccaagcagtg ggtgcgcgac tacaccgggc cgaagttcga cagcggcgag ctggtcaagg
     4261 ccgagcttgc gcaccgcaac aacgccggca tgcaggcctt cgcgttcaac ctgcgccggc
     4321 cgctgttcca ggacatccgc gtgcgccggg cgctggcgct ggccttcgac ttcgaatggt
     4381 cgaaccagac gctgttcgag ggcctgtacc ggcgcagcaa cagctacttc accaacagcg
     4441 acctggccgc gagcggcgtg ccgcagggcg cggaactcgc cctgctcgaa ccgttccgcg
     4501 cgcagctgcc ggcggagctg ttcgagcggc cctggcagcc gccgtcgacc gaggcgccgg
     4561 ggtcgctgcg cgacaacctg cgcgcggcgc tggcgctgct gaaggaggcc ggctggacgc
     4621 tcgccgacaa cgtgctggtc aaggatggcc gcaggctcga attcgagatg ctgctggtca
     4681 accgcggctt cgagcgcgtg atcgcgccgt acgcgaagaa cctcgaacgc ctcggcgtgc
     4741 gcctgaacta ccgcaccgtc gatacctcgc tgtaccagca gcgcgtcgac aactttgact
     4801 tcgatgcgct cgtgcaggtc tggccgcagt cgccgtcgcc cggcaacgag cagtccggca
     4861 actggggctc ggcgagcgcc gaccagcccg gttcgaacaa cctgctcggc ctgaaggacc
     4921 cggtcgtcga cgcgctgctc gcgaagctcg tctatgcgcc ggatcgcgcc gcgctggtca
     4981 cggccgcgca cgcgctcgat cgcgtgctgc tgtggggcga ttacgtcgtg ccgaactggt
     5041 attcgccggt gcaccggctg gcctggcgca accgcttcgc gcatccgcag acgctgccgc
     5101 tgtactacca gccgatcgac tgggtcatca gcagctggtg ggcgaagtag ggcatggccg
     5161 cctatctcgc ccgccgcgtg ctgctgatgg tgccgacgct gctcggcatc ctgctggtca
     5221 gcttcacgat catccagttc gtgcccggcg gcccggtcga gcaactggtc aacgagctga
     5281 cgcaccgcgg tgctgccggc gaggcgagca gcggcggcgg cagcggcgtc gcctaccgcg
     5341 gcgcgcaggg gctcgggccg caggaggtcg aggcgatccg caggctctac ggcttcgaca
     5401 agccggccat cgagcgcttc gccgacatgg tcgtgcgcta tgcgcgcttc gatctcggtg
     5461 acagctactt ccaccaccgc tcggtgctcg ggctgatcgc cgacaagctg ccggtgtcgg
     5521 tgagcctcgg cgtctggagc ttcctgatcc tgtacgcggt gtcgatcccg ctcggcatcc
     5581 gcaaggcgct gcaggacggc tcgcgcttcg atgtcgccac cagcacgctg atcctggtcg
     5641 gctacgcgat ccccggcttc gtgctcggcc tgctgctgct ggtgctgttc ggcggcggca
     5701 gcttctggca gctgttcccg ctgcgcggcc tgacctcgga gggctgggag cagctgccgt
     5761 ggtatgcgca ggtgctcgac tacctgtggc acctggtgct gccgatcacc gcctcgaccg
     5821 tcggcggctt cgcggtgatg acgctgctga ccaagaacag cttcctcgaa gagatccgca
     5881 agcagtacgt gctgacggcg cgcgccaagg gcctgaccga gcgcgccgtg ctgtaccggc
     5941 acgtgttccg caacgcgatg atcccgctgg tcaccggctt cccggcggcg ttcatcggca
     6001 tgttcttcac cggcagcctg ctgatcgaga cgaccttctc gctcgatggc ctggggctgc
     6061 tgtcctacca gtcggtgctg cggcgcgact atccggtggt catgggcacg ctgtacctgt
     6121 acacgctgct cggcctgctc gcgaagctgc tgtcggacct gtcctacgtg ctggtcgatc
     6181 cgcgcatcgg cttcgaatcg gtggaccggt gagcgcgccg gcggcagatc ccgcgccgct
     6241 ggcggccggc ctgtcgccgc gcgcgcgggc ctggcggcgc tttcgcgcca accgccgcgg
     6301 ctggtggagc ctgtggatct tcggcgtgct gttcggactc agcctcggcg ccgagctgct
     6361 gtcgaacgac cggccgctgc tcgtgcacta cgagggcgcg tactacttcc cgctgctgaa
     6421 gacctatccg gagaccgcgt tcggcggcga cttcgacacc gaggccgact accacgaccc
     6481 ctacctgcgc gagcggctgg ccgggggcga caactggctg ctcgatccgc cgaaccccta
     6541 ccgctacgac acgatccgcc tcgacggcca gcgcgccaa
//
LOCUS       DAOQIE010000230         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6361_length_6518_cov_7.802568, whole genome shotgun sequence.
ACCESSION   DAOQIE010000230 DAOQIE010000000
VERSION     DAOQIE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_6518_cov_7.802568"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK144_13450"
     CDS             complement(<1..416)
                     /locus_tag="PK144_13450"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNF66778.1"
                     /translation="MQAPILRVAVPSPLPAALDYLAPPGPLPPLVPGLRVRVPLKNGE
                     RIGVLLDTAGASELASTKLKPALAILDDTPLLPAELLALGHWAAGYYHHPPGEVFATL
                     LPAALRDGQPPLRRCEPGFRITEAGRLALQEQATGRS"
     gene            587..802
                     /locus_tag="PK144_13455"
     CDS             587..802
                     /locus_tag="PK144_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66779.1"
                     /translation="MGKKGKKKDRDEEAKFVIRIEKDLRDEFVDLCRDLDTTASREVR
                     RFIRSYLAENGRGASDAPPSPDAGESA"
     gene            833..1006
                     /locus_tag="PK144_13460"
     CDS             833..1006
                     /locus_tag="PK144_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66780.1"
                     /translation="MNIDRNDSPVHAQTVAAIEAQQAYFEARARGVSRAERERLERNW
                     LSAARRLRIGMAQ"
     gene            complement(1125..1808)
                     /locus_tag="PK144_13465"
     CDS             complement(1125..1808)
                     /locus_tag="PK144_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF66781.1"
                     /translation="MTQKKNLAVLAAITLSLAVAGCAQPGAGGGGSSSGSGMSSTATG
                     AVAGAALGALAGGLSGKHDKAKRALIGAGVGALAGAAVGSYLDKQKNDLDAQLAGSGI
                     GVERKGDSIVLNLPEAITFDSGQAALKPAAEPQIDRIAAVLKEYPQTVVDIAGHTDST
                     GAAEANQALSQKRAGAVALRFVKAGLPYARMVVSGYGATRPVADNASEAGRAQNRRVE
                     ITLLPVTQG"
     gene            complement(2264..2941)
                     /locus_tag="PK144_13470"
     CDS             complement(2264..2941)
                     /locus_tag="PK144_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNF66782.1"
                     /translation="MSELLPTVEIEPAVPATATVIWLHGLGADGHDFEPVVPELGLPP
                     GHTIRFVFPHAPRRPVTVNGGFVMRAWYDILGFDGPVSEDAAGIRASAAQITALIARE
                     NARGIPDARIVLAGFSQGAAMALHTGLRHRERLAGLLALSGYLPLAATLAAERSPANA
                     ATPVFLAHGEIDAVVRFPRGTATRDRLVQLGWQPEWHDYPIGHEVCWEELRDIGAFLN
                     RVLPARP"
     gene            3039..3179
                     /locus_tag="PK144_13475"
     CDS             3039..3179
                     /locus_tag="PK144_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNF66783.1"
                     /translation="MPRTVSARSVLLVLAALLAFTVAGCGQKGDLYLPGGKPQKVERK
                     PT"
     gene            3226..4476
                     /gene="lysA"
                     /locus_tag="PK144_13480"
     CDS             3226..4476
                     /gene="lysA"
                     /locus_tag="PK144_13480"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNF66784.1"
                     /translation="MDHFEYRQGQLYAEDVPVADLARRFGTPCYVYSRATLTRHFRVF
                     DDAFGSHPHLVCYAVKANGNLGVLNVLARLGSGFDIVSVGELERVLAAGGDPGKVVFS
                     GVGKREDEIARALEVGIRCFNVESEAELERLQQVAAAHGVQAPVSLRVNPDVDAGTHP
                     YISTGLKENKFGIAIADAPAVYARAASRANLRVTGVDCHIGSQLTTLAPFTDTLDRLL
                     LLVERLAADGIGIDHIDLGGGIGIRYRDEQPPHPSQHIAAVLERLAGLPYAVYVEPGR
                     AIVGNAGLLLTRVEFLKCSEHKNFAIVDAAMNDLIRPSLYSAWMEIIPADATLERAQQ
                     RYDVVGPICETGDFLGKDRELAVAEGDLLAVRSAGAYGFTMSSNYNARPRAAEVLVDG
                     SEVHLVRARESLASLYAGESVLPA"
     gene            4473..4901
                     /locus_tag="PK144_13485"
     CDS             4473..4901
                     /locus_tag="PK144_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNF66785.1"
                     /translation="MSFTNPGPDAIRARLAQVRSIAVVGQSDSPGRPSYGVALGLQGL
                     GYRIIPVNPRLNHALGLPAYASLDELPAVPDLVDVFRASEHVAGIVDDCIRLGVPALW
                     LQDGVIDAAAARRAQAAGIFVVMDRCLWRDCVQLGVRAAC"
     gene            5030..6244
                     /locus_tag="PK144_13490"
     CDS             5030..6244
                     /locus_tag="PK144_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66786.1"
                     /translation="MKHACLIAGALYAAMTMPTQAKDLFELGLSVDSAPEVREGFTTI
                     SDLFDALSTEGLTQLSPGYTESSPATAALYVRGLPAEASFQSGSTTLRFRVPSLGIDV
                     SFTGETRDDSRDQFEDYIKANGGDILKRMLKELARVSPVDPVAGNPGSLMAQMGAQDF
                     ADAASVSGNPGGTGSSGNLFGIGLGYDSNSLGPYDQNVMRLPLSFGFDLSNGYRLTLS
                     APIYRVESEGTDSYGASFGAALRIPLTADWALTPALRVGAAGSGDLGSAAALYSGSLT
                     SRYVWHFAGGLDLGMTNQLALYQSRSISAGDYEADYDLSNRSLKNGLDLAGPLNGTFL
                     GNGAQWRAWVIDTRFYGDELYSNSWQEYGVSVGTRVQAGAIVYEQMNLGVSYLNGEND
                     VSGLKLNFGYRF"
BASE COUNT         1019 a         2293 c         2218 g          988 t
ORIGIN      
        1 gagcggccgg tcgcctgctc ctgcagcgcc agccggccgg cctcggtgat ccggaagccc
       61 ggctcgcagc ggcgcagcgg tggctggcca tcgcgcagcg ccgccggcag cagcgtcgcg
      121 aacacctcgc cgggcggatg gtggtaatag ccggccgccc agtgaccgag cgcgagcagt
      181 tcggccggca gcagcggcgt gtcgtcgagg atcgccagcg ccggcttgag cttcgtcgag
      241 gcgagttcgc tggcgccggc cgtatccagc agcacgccga tgcgttcgcc gttcttcagc
      301 ggcacgcgca cgcgcagccc cggcaccagc ggcggcagcg gaccgggcgg tgcgaggtaa
      361 tcgagggcgg caggcagcgg cgagggcacg gcgacgcgca ggatcggggc ttgcatcccg
      421 gcattgtagg ggccggcgcc gggggcgggc cggcgcgggt tttcatgcat ccgtcacgaa
      481 accctcacgg ttcctgcaca aattcttcac gaaacattca cttgtccggt aggcacagcg
      541 tgtagatact gtgcccacgt cgtctataca aaaaagtcat ccgggcatgg gcaagaaggg
      601 caagaagaag gacagagacg aagaagcgaa attcgtcatc cgcatcgaaa aggaccttcg
      661 cgacgaattc gtggacctct gccgcgacct ggatacgacg gcgtcacgcg aggtgcggcg
      721 cttcatccgc agctacctcg ccgagaacgg caggggcgca tcagacgccc cgccgtcccc
      781 tgatgcaggg gaaagcgcgt aattccattg gcacggctct ggaggcgaaa ccatgaacat
      841 cgaccggaac gattcacccg tccacgccca gacggtcgcg gcaatcgagg cccagcaggc
      901 ctacttcgag gcccgcgccc gcggtgtctc gcgcgccgaa cgtgagcggc tcgaacgcaa
      961 ctggctgtcg gccgcgcgcc gcctgcgcat cggcatggcc cagtgaggcc agcgccgggg
     1021 gcacggcggc ccgcggcaca gctgaagaag gccggctcct gccatcgcgg cagggccggc
     1081 cttcactttt tgcggcgtgg cgagcggcgt gatccggcgc cggatcagcc ctgcgtgacc
     1141 ggcagcagcg tgatctcgac gcgacggttc tgcgcgcgcc cggcctcgct ggcgttgtcg
     1201 gcgaccggac gcgttgcgcc gtagccggag accaccatgc gcgcatacgg caggccggcc
     1261 ttgacgaagc gcagcgccac ggcaccggcg cgcttctgcg acagcgcctg gttggcctcg
     1321 gccgcgccgg tcgaatcggt atggccggcg atgtcgacga cggtctgcgg atattccttc
     1381 agcaccgcgg cgatgcggtc gatctgcggc tcggccgccg gcttcagggc cgcctgaccg
     1441 ctgtcgaagg tgatcgcctc gggcaggttc agcacgatgc tgtcgccctt gcgctcgaca
     1501 ccgatgccgc tgccggccag ctgcgcatcc agatcgttct tctgcttgtc gagatagctg
     1561 ccgaccgccg cgccggcgag cgcgccgacg ccggcgccga tcagcgcacg cttggccttg
     1621 tcgtgcttgc cgctgaggcc gccggccagg gcgccgagcg cggcaccggc gacggcaccg
     1681 gttgcggtgc tgctcatgcc gctgccgctg ctgctgccgc caccgccggc accgggctgg
     1741 gcgcagccgg ccacggcgag cgagagcgtg atggcggcca gcacggccag gttcttcttc
     1801 tgggtcatgg agtcttcctc tgcttcccgg ggaaaccggc gcccgttgcg gtgggccggt
     1861 gcgaggacgt cgcgagggtc atgacaggca aggaccgcgc aggtgcgacg cccggatcgg
     1921 ggcaaaagaa tagccggtgt caccagaggg acaccggctt gaaggcgggg tgggtcttgt
     1981 gacggggccg gacgacctcc tgccgaccgg gaacctcgtc tgttatggct gctgatgcca
     2041 gcgcaaaagc atcttgcatg ccaatctttt cattgcatca gaacaattac ttgccgggtg
     2101 cccgcggcgc agccgccggt tttccggcgc cggcgtccgc aaaacggcgt caaaaatgca
     2161 aggtcgatgg catttcggga atgcagcggc gggcggggtg ctgcaatccg cagcccgccg
     2221 gcgagctgca gcgcaggatg ccagcgggct cccgttcacc ggctcagggc cgggccggca
     2281 gcacgcggtt caggaaggcg ccgatgtcgc gcagttcctc ccagcagacc tcgtggccga
     2341 tcggatagtc gtgccattcg ggctgccagc cgagctgcac cagccggtcg cgcgtcgccg
     2401 tgccgcgcgg gaagcgcacg acggcatcga tctcgccgtg cgcgaggaat accggcgtcg
     2461 cggcgttggc cgggctgcgc tcggcggcga gcgtcgcggc aagcggcaga tagcccgaca
     2521 gcgccagcag cccggccagg cgttcccgat ggcgcaggcc ggtgtgcagc gccatcgccg
     2581 cgccctgcga gaagccggcg agcacgatgc gcgcatccgg gatgccgcgg gcgttctcgc
     2641 gcgcgatcag cgccgtgatc tgcgcggccg aggcgcggat gccggcagca tcctcgctga
     2701 ccgggccatc gaagccgagg atgtcgtacc aggcgcgcat cacgaaaccg ccgttgacgg
     2761 tcaccggccg gcgcggcgcg tgcgggaaca cgaagcggat cgtgtgtccc ggcggcaggc
     2821 cgagttccgg caccaccggc tcgaagtcgt ggccgtcggc gccgagcccg tgcagccaga
     2881 tcacggtggc ggtcgccggt acggcgggtt cgatttcgac ggtcggcagc agctcgctca
     2941 tctcgggaca cctgtcggta cggaggggga agggtgcagt gtaagcgccg cggtatactc
     3001 gccggcccat ccgcctgccg tcgccggaaa cctccgccat gccccgcacc gtgtccgccc
     3061 gttccgtgct gctcgtcctc gccgcgctgc tggcgttcac cgtcgccggc tgcggccaga
     3121 agggtgacct ctacctgccg ggcggcaagc cgcagaaggt cgagcgcaag ccgacctgag
     3181 ccggccgcag ccgcccgccg ttccgtccac ccggggatcc gcgtcatgga ccatttcgaa
     3241 taccgccagg gtcagctgta cgccgaagac gtgccggtcg ccgacctcgc ccggcgcttc
     3301 ggcacgccgt gctacgtgta ctcgcgcgcg acgctgaccc ggcatttccg cgtcttcgac
     3361 gacgccttcg gcagccatcc gcacctggtc tgctacgcgg tcaaggccaa cggcaacctc
     3421 ggcgtgctga acgtgctcgc gcgcctcggt tcgggcttcg acatcgtctc ggtcggcgag
     3481 ctggagcgcg tgctggccgc cggcggtgat ccgggcaagg tggtgttctc cggcgtcggc
     3541 aagcgcgagg acgagatcgc ccgcgcgctg gaggtcggca tccgctgctt caacgtcgag
     3601 agcgaggccg aactcgaacg tctgcagcag gtcgccgccg cgcacggcgt gcaggcgccg
     3661 gtcagcctgc gcgtgaaccc cgatgtcgac gccggcacgc atccgtacat ctctaccggc
     3721 ctcaaggaga acaagttcgg catcgccatc gccgatgcac cggccgtcta cgcccgcgcc
     3781 gccagccggg cgaacctgcg cgtgaccggg gtcgactgcc acatcggctc gcagctgacc
     3841 acgctcgcgc cgttcaccga cacgctcgac cggctgctgc tgctggtcga gcggctcgct
     3901 gccgacggca tcggcatcga tcacatcgac ctcggcggcg gcatcggcat ccgctatcgc
     3961 gacgagcagc cgccgcaccc gagccagcac atcgccgcgg tgctcgaacg cctggccggc
     4021 ctgccgtacg cggtctacgt cgagccgggc cgtgcgatcg tcggcaacgc cgggctgctg
     4081 ctgacgcgcg tcgagttcct gaagtgttcg gaacacaaga acttcgcgat cgtcgatgcg
     4141 gcgatgaacg acctgatccg gccgtcgctg tactcggcgt ggatggaaat catcccggcc
     4201 gacgccacgc tcgaacgcgc gcagcagcgc tacgacgtgg tcggcccgat ctgcgagacc
     4261 ggcgacttcc tcggcaagga ccgcgaactc gccgtggccg aaggcgacct gctggccgtg
     4321 cgctcggccg gggcctacgg cttcacgatg agcagcaact acaacgcccg cccgcgcgcc
     4381 gccgaggtgc tggtcgacgg cagcgaggtc catctggtgc gcgcgcgcga atcgctggcg
     4441 agcctctatg ccggcgaatc ggtgctgccg gcatgagctt caccaacccc ggccccgatg
     4501 ccatccgcgc ccggcttgcg caggtgcgca gcatcgccgt cgtcggccag tcggattcgc
     4561 cgggccggcc gagctacggt gtcgccctcg gcctgcaggg gcttggctac cggatcatcc
     4621 cggtcaaccc gcggctgaac cacgccctcg gcctgcccgc gtacgcctcg ctcgacgaac
     4681 tgccggccgt gccggatctg gtcgacgtgt tccgcgcctc cgaacacgtc gccggcatcg
     4741 tcgacgactg catccgcctc ggcgtgccgg cgctgtggct gcaggacggc gtcatcgatg
     4801 ccgccgccgc gcgccgtgcg caggcggccg gcatcttcgt ggtcatggac cgctgcctgt
     4861 ggcgcgactg cgtgcagctc ggcgtgcgtg cggcctgctg atcgacatga gttgcaattc
     4921 agcaactgtg cttaacagct acacaacaac tcctatcatc cccgcaacgc caccatatcc
     4981 gcaggtggcc tgaccgcaat ccgcagcgga cccgggggag acaaggaaca tgaagcacgc
     5041 ctgcctgatc gccggcgcac tgtacgccgc gatgacaatg cccactcagg ccaaagacct
     5101 gttcgagctg ggcctgagcg tcgacagcgc gcccgaggtg cgcgaaggct tcacgacgat
     5161 cagcgacctg ttcgacgccc tctccaccga agggctgacg caactgtcgc ccggctatac
     5221 ggaatcctcg ccggcgactg ccgcgctgta cgtgcgcggc ctgccggccg aagcctcgtt
     5281 ccagtccgga tcgacgacgc tgcgctttcg cgtgccctcg ctcggcatcg acgtgagttt
     5341 caccggcgaa acccgcgacg acagtcgtga ccagttcgag gactacatca aggccaacgg
     5401 cggcgacatc ctcaagcgca tgctgaagga gctggcccgt gtgtcaccgg tcgacccggt
     5461 cgccggcaat ccgggcagtt tgatggcgca gatgggggcg caggattttg ccgatgccgc
     5521 cagtgtcagc ggcaatcccg gcgggaccgg ttcctcaggc aacctgttcg gcatcggtct
     5581 cggctatgac agcaactcgc tcggtcccta tgaccagaac gtcatgcgcc tgccgctgag
     5641 cttcggcttc gacctgagca acggctaccg gctgacgctg tcggcaccga tctatcgcgt
     5701 cgaatccgaa ggcaccgaca gctacggcgc gagcttcggc gcggcactgc gcattccgct
     5761 gacggccgac tgggcgctga cgccggcgct gcgcgtcggt gcggccggct ccggcgatct
     5821 ggggagtgcc gccgcgctgt actcgggctc gctgaccagc cgctacgtct ggcatttcgc
     5881 cggcggactc gatctcggca tgaccaacca gctcgcgctc taccagtcgc gctcgatcag
     5941 cgccggtgac tacgaggccg actacgacct gtcgaaccgc agtctcaaga acggcctcga
     6001 cctcgccggg ccgctgaacg gcaccttcct cggcaacggc gcccaatggc gggcctgggt
     6061 catcgacacg cgcttctacg gcgacgagct gtactcgaac tcctggcagg aatacggcgt
     6121 gtcggtcggc acgcgcgtgc aggccggcgc gatcgtctac gagcagatga acctcggcgt
     6181 cagctatctc aacggcgaga acgacgtgag cggcctgaag ctgaacttcg gctaccggtt
     6241 ctgaggcagg cggccgcaat ccgcccgctg ctgcataccc ttgcatccgg aatcggcatc
     6301 cggagcatcc gcggcgggcg catcgtcccg aagcccgccc gcatctgcac tgatccggac
     6361 agtttccggc aattgatcta tataaggacg cgcacatcgt ccgcggtggc ccggccgtgt
     6421 cccacgaccg gtcgcggccg gccacccgcc atcgaggaaa acccgaggtc tccgtctgcg
     6481 ccgcaaagga ctgcagcgca gccagtccgt tgatcttc
//
LOCUS       DAOQIE010000231         6501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6392_length_6501_cov_7.074155, whole genome shotgun sequence.
ACCESSION   DAOQIE010000231 DAOQIE010000000
VERSION     DAOQIE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_6501_cov_7.074155"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..424)
                     /locus_tag="PK144_13495"
     CDS             complement(164..424)
                     /locus_tag="PK144_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004386309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNF66787.1"
                     /translation="MGFFSWILFGLIAGAIAKLIMPGRQGGGFILTVLLGVVGALVGG
                     WMGTVMGFGTLSGFDLRSLAIAIAIAGALVVLFLFGMARRAD"
     gene            515..991
                     /locus_tag="PK144_13500"
     CDS             515..991
                     /locus_tag="PK144_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66788.1"
                     /translation="MPMQFATGLSLQRLPDTAAGLPRAALLRDGRQNGCIIDGTLLEA
                     ALRWPDGWTLLIASADCPFEESLHLSLLDAHDHIVDCAGIGMAYASGWLRDLNLSPPD
                     SVRFGFLGDGEWSVRRLHERGWRMPLFSEPAGVWRRFGWRRWFVIGRHESGDIGTD"
     gene            1512..1856
                     /locus_tag="PK144_13505"
     CDS             1512..1856
                     /locus_tag="PK144_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015608329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF66789.1"
                     /translation="MAFMVNQRNVDQVMILDAAGKLTIGEGDIKLRISINDSLNAGAR
                     NLLVNLAGVSTIDEDGVGELVRAYVTTSDRGGKLKLLNLPPKIQDVLTITQLITMFEV
                     YDNEQQAVASFG"
     gene            complement(1894..2922)
                     /locus_tag="PK144_13510"
     CDS             complement(1894..2922)
                     /locus_tag="PK144_13510"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I asparaginase"
                     /protein_id="HNF66790.1"
                     /translation="MSDASPRARVWIAYTGGTLGMVPGTDGYVPAPGLEARLRAALPQ
                     LDAPGVPAFELYEYAELLDSANMSTADWNRIGADLAARQAGYDGFVVVHGTDTMAYTA
                     SALSFMLEGLTKPVILTGSQIPLCAARSDARDNLLDALILAGSSAIPEVMIAFGGTLL
                     RGNRTTKVQSEGLRAFASPNAGALAELGIDIRIHRERVRSPAGVPFRLRPYSSAEVAA
                     VRLFPGITGDFLARLLAPPLAGLVLEAYGAGNGPARDPAILAALAAACARGVVVVVVT
                     QCLEGRAELGVYAAGSALARAGCIGGADMTAEAALTKLAYLFAVGLDPAAVRAEIARD
                     LRGELTAD"
     gene            complement(2919..4376)
                     /locus_tag="PK144_13515"
     CDS             complement(2919..4376)
                     /locus_tag="PK144_13515"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNF66791.1"
                     /translation="MSTALVWFRRDLRLADNPALSAACREHERVLPVFIHAPDEEAPW
                     APGAASRWWLQHSLTALGEALARKGVPLVIRRGPTREALQALIRETGATAVYWNRLYE
                     PAPVARDTGLKRALREQGIRAESFDGNVLHEPWAVMNGSGEPYRVFTPYWKAVARMEQ
                     PRPLPEPAALPGPALRTPSLALAELGLLPSIPWDKGLREAWTPGEAGARARLAAFLDG
                     IWIDYDSARNLPGRPGVSRLSPHLHFGELSPRQVWAAIGARCGNQPLESHGGESFLRE
                     LAWREFAIYVLWHWPQSADQPLQARFAEYPWAEDYARPLAAWQHGRTGYPLVDAGMRE
                     LWTTGWMHNRVRMMVASFLTKNLRIPWQEGARWFWDTLVDADLASNSLGWQWTAGCGV
                     DAAPYFRVFNPVRQGEQYDGDGAYVRRWLPELDPVPDKYLHAPWALPAAEAARVGYHS
                     GINYPEPLVDFAASRALALEGYERVKAGGARGLSA"
     gene            complement(4518..4763)
                     /locus_tag="PK144_13520"
     CDS             complement(4518..4763)
                     /locus_tag="PK144_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02647 family protein"
                     /protein_id="HNF66792.1"
                     /translation="MSHRLSAETCAELQLLALFSTATQQSGLKVHHSAAPELVAAATR
                     LHEKRLITQPDGGYLTDLGYEAANGLDAVLDILGAPG"
     gene            complement(4791..5744)
                     /locus_tag="PK144_13525"
     CDS             complement(4791..5744)
                     /locus_tag="PK144_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNF66793.1"
                     /translation="MHGVFLDRASLDLGDLDFSALTHALDRFTGYAATAPDEVAVRIR
                     GADIVIVNKVVLDAATLATAAALKLICVAATGTNNVDLEAARARGIPVVNCRGYGTPA
                     VAQHAILLMLALSTNFLAYQRDLAGGAWPRAAQFCLLDHPIRELAGKVLAVVGWGELG
                     QGTARLAEAFGMDVRIAQLPGRPAGPGRVPLDELLALADVVTLHCPLNDTTRNLFDAA
                     RLARMKPTALLINTARGGLVDEAALAGALRAGRLGGAGFDVLSTEPPRAGNPLLAADI
                     PNLIVTPHNAWGSVESRQRMLGQIAENIRAWRGGSPLRVVV"
     gene            5855..>6501
                     /gene="hemH"
                     /locus_tag="PK144_13530"
     CDS             5855..>6501
                     /gene="hemH"
                     /locus_tag="PK144_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNF66794.1"
                     /translation="MSGPDSRASAPVHVHGRAETTGILLVNLGTPDAPTPTALRRYLA
                     EFLWDRRVVEIPRPLWWLILHGIILRTRPAKSAAKYATVWTPEGSPLLAIGRRQQAGL
                     QARLAAGDHGPLQVALGMRYGQPSIATALAELRAAGCTRLLVLPLYPQYSAATTATAF
                     DALAAELARWRRLPELRCVMQYPDEPAWVDAVAASLDQHAAAHGGLAERVLFSFHG"
BASE COUNT         1021 a         2222 c         2321 g          937 t
ORIGIN      
        1 agggctattt gatcaaggtg ttgaacgggc agatgattta gatgcgtttg ccctgcccct
       61 cccccgtcca tcgccggtcg agcggtagcg accggcgatg gacggcaggg caatgcagcc
      121 cgacggggcc gtaaacctga ccacgacccg agcaacgcga gcgtcaatcc gccctgcgcg
      181 ccatcccgaa caggaacagc acgaccagcg cgccggcgat ggcgatggcg atggcgaggc
      241 tgcgcaggtc gaagccgctc agcgtgccga agcccatgac cgtgcccatc cagccgccga
      301 ccagcgcgcc gaccacgccg agcagcacgg tcaggatgaa gccgccgccc tgacggccag
      361 gcatgatgag tttggcgatg gcgccggcga tcagtccgaa caggatccag ctgaaaaagc
      421 ccatgatgat gactccggag aaagggttcg aggcagaacc gggcctgcgt tctgacgggc
      481 ggctccggcc gaaattccct gcccgtccgg cccgatgcca atgcagtttg ccaccggcct
      541 gagcctgcag cgcctgcccg acacggccgc cggcctgccg cgtgccgcct tgctgcgcga
      601 cggtcgccag aacggctgca tcatcgacgg cacgctgctc gaagctgcac tgcgctggcc
      661 ggatggctgg acgttgctga tcgccagtgc cgattgcccg ttcgaggaat cgctgcattt
      721 gtcgctgctc gatgcgcacg accacatcgt cgattgcgcc ggcatcggca tggcttacgc
      781 cagcggctgg ttgcgggacc tgaatctgtc accacccgac agcgtgcgct tcgggtttct
      841 cggcgacggc gaatggtcgg tgcgccgcct gcacgagcgt ggctggcgaa tgccgctgtt
      901 cagcgaaccg gcaggcgtct ggcgccgttt cggctggagg cgctggttcg tgatcgggcg
      961 acatgaatcc ggcgacatcg gcacggactg aatccggaaa ccgggaatgt ttcccgacgc
     1021 aatcgctgcc cgcgcgcatc ctcccgacat gacacgcgca gctgcgtcca ccagcccgat
     1081 tcagccctga ccgcaagccg ctctttcacc aatgccgtcc gcgtcaagag caccttgcat
     1141 tacgataccg acatgcgcat gaaacatcag acatccccaa cccagctatc gccataaatc
     1201 gctgcaagct gctgattacc gtccataaat acacaatacc cttatgcact cagtcaactt
     1261 gccgccctcg ccggccagcg gctagagtcc ggaccgcaga ctgttccggg caggatcggg
     1321 aatccgccct gccagtccga caccgcaatg ccggcgctca tgttgaccgg caattatttc
     1381 cggcaccgga ttccatgcgc tcctgtccaa aacagcctgc acgatcgaat ccggcttgca
     1441 tgtgttctgc actgcatcgg cagaacctgc aactgtatcc aaatcagtat ctgcaacgga
     1501 gaaggaatat catggcattc atggtcaatc agcgtaacgt cgaccaggta atgatcctgg
     1561 atgccgctgg caagctgacg atcggcgaag gcgacatcaa gttgcgtatc tccattaacg
     1621 acagtctgaa tgccggtgcg cgcaatctgt tggtcaatct cgccggggtc agcaccattg
     1681 acgaggatgg cgtcggcgaa ctggtcaggg cttatgtcac gaccagcgat cgaggcggga
     1741 aactcaaact gctgaacctg ccgccgaaaa tccaggacgt gctgacgatc acgcaactga
     1801 tcaccatgtt cgaggtgtac gacaacgagc agcaagccgt ggcgagcttc ggctgacgac
     1861 ccgtgcgggc acggctccgc agtggcgccg gcttcagtct gccgtcagct cgccgcgcag
     1921 atcgcgcgcg atttcagcgc gtaccgcggc cggatcgagg ccaacggcga acaggtaggc
     1981 gagcttggtc agcgccgctt cggcggtcat gtcggcgccg ccgatgcagc cggcgcgcgc
     2041 cagtgccgag ccggccgcgt agacgccgag ttcggctcga ccttcaaggc attgcgtgac
     2101 cacgaccacg accacgccgc gcgcgcaggc cgcggcgagt gcggcaagaa tcgccgggtc
     2161 gcgcgccggg ccgttgccgg cgccgtaggc ttcgagcacg aggccagcga gcggcggcgc
     2221 cagcaagcgc gcgaggaaat cgccggtgat accggggaat aaacgcacgg cggcgacttc
     2281 agcactgctg tacgggcgca gccggaacgg cacaccggcc gggctgcgca cgcgttcgcg
     2341 gtggatgcgg atgtcgatgc cgagttcggc aagcgctccg gcgttcggcg aagcaaaggc
     2401 acgcaggccc tcggactgca ctttcgtcgt gcggttgccg cgcagcagcg tgccgccgaa
     2461 cgcgatcatg acttcgggga ttgcactgct gccggcgagg atcagcgcat cgagcaggtt
     2521 gtcgcgggca tcgctgcgtg ccgcgcagag cggaatctgc gagccggtca ggatcaccgg
     2581 cttcgtcagc ccttcgagca tgaacgacag cgccgaggcg gtgtaggcca tcgtgtcggt
     2641 gccgtgcacg acgacgaagc cgtcgtaccc ggcctggcgg gcggcgagat cggcgccgat
     2701 gcggttccag tcggccgtgc tcatgttggc gctgtcgagc agctcggcgt actcgtacag
     2761 ctcgaaggcc ggcacgccgg gcgcatcgag ctgcggcagc gcggcgcgca gccgcgcttc
     2821 gaggcccggc gccggcacgt agccgtcggt gcccggcacc atgccgagcg tgccgccggt
     2881 gtaggcgatc cagacgcgcg cccgcggact ggcgtcgctc atgccgacag gccgcgggcg
     2941 ccgccggcct tgacgcgctc gtagccttcg agggcgagcg cgcggctcgc agcgaaatcg
     3001 acgagcggct ccggatagtt gatgccggag tggtagccga cgcgcgcggc ctcggcggcc
     3061 ggcagcgccc acggggcgtg caggtatttg tcgggcaccg gatcgagctc gggcagccag
     3121 cggcgcacgt aggcaccgtc gccgtcgtac tgctcgccct ggcgcaccgg gttgaacacg
     3181 cggaagtacg gcgcggcatc gacgccgcag ccggccgtcc actgccagcc gaggctgttc
     3241 gaggcgaggt cggcatcgac gagggtgtcc cagaaccagc gcgcgccctc ctgccacggg
     3301 atgcgcaggt tcttggtcag gaaggaggcg accatcatgc gcacgcggtt gtgcatccag
     3361 ccggtggtcc agagctcgcg catgccggca tcgaccagcg gatagccggt gcgcccgtgc
     3421 tgccacgcgg cgagcggacg cgcgtagtcc tcggcccagg ggtattccgc gaagcgcgcc
     3481 tgcagcggct gatcggcact ctgcggccag tgccagagca cgtagatcgc gaactcgcgc
     3541 caggccagct cgcgcaggaa gctctcgccg ccgtgcgatt cgagcggctg gttgccgcag
     3601 cgcgcgccga tcgcggccca gacctgacgc ggcgacagct cgccgaagtg caggtgcggc
     3661 gacagccgcg acacgcccgg ccggcccggg aggttgcggg cgctgtcgta gtcgatccag
     3721 atgccgtcga gaaaggccgc gagccgcgcg cgcgcgccgg cctcgcccgg cgtccaggcc
     3781 tcgcgcaggc ccttgtccca cgggatcgac ggcagcaggc cgagttcggc gagggccagc
     3841 gacggcgtgc gcagcgccgg ccccggcagc gcggccggtt ccggcagcgg ccgcggctgc
     3901 tccatgcgcg cgaccgcctt ccagtacggc gtgaacacgc ggtacggctc gccgctgccg
     3961 ttcatgaccg cccagggctc gtgcagcacg ttgccgtcga agctctcggc gcggatgccc
     4021 tgctcgcgca gcgcgcgctt gaggccggtg tcgcgcgcga ccggcgccgg ctcgtacagg
     4081 cggttccagt agacggccgt cgcgccggtc tcgcggatca gcgcctgcag cgcctcgcgc
     4141 gtcggtccgc ggcggatcac cagcggcacg cccttgcgcg ccagtgcctc gccgagcgcc
     4201 gtgagcgaat gctgcagcca ccagcggctg gcggcacccg gcgcccaggg cgcctcctcg
     4261 tccggggcgt ggatgaagac cggcagcacg cgttcatgct cgcgacaggc ggcgctcagc
     4321 gcggggttgt cggcgaggcg caggtcgcgc cggaaccaga cgagggcggt actcatggca
     4381 ggaacgggct ccgtgggcgg gtcgacgggg acccgcgcac ggacggcgcg ggccgacgga
     4441 aacggcaggg tgacagggag tatggacgag ggagtatgga cggtcgtaca ggttcaagcg
     4501 ctccggcgtg cgggttgtca gccgggcgcg ccgaggatgt cgagcaccgc gtcgaggccg
     4561 ttggcggcct cgtagccgag gtcggtcagg taaccgccgt cgggctgggt gatcaggcgc
     4621 ttttcgtgca ggcgcgtggc ggcggcgacc agttcgggcg cggcgctgtg gtgaaccttc
     4681 agtccggact gctgcgtggc cgtgctgaac agcgcgagca gttgcagttc ggcacaggtt
     4741 tcggcgctca ggcgatggct caagggcggt gctcctgttg cggtgaggat tcagacgacg
     4801 acgcgcagcg gcgagccgcc gcgccaggcg cggatgttct cggcgatctg gccgagcatg
     4861 cgctggcgcg actcgacgct gccccaggcg ttgtgcggcg tgacgatcag gttcgggata
     4921 tccgcggcca gcagcggatt gccggcgcgc ggcggttcgg tgctcagcac gtcgaagccg
     4981 gccccgccga ggcgcccggc gcgcagggcg ccggcgagcg cggcctcgtc gaccagcccg
     5041 ccgcgggcgg tgttgatcag cagcgccgtc ggcttcatgc gcgccagccg cgcggcgtcg
     5101 aacaggttgc gcgtcgtgtc gttcagcggg cagtgcagcg tgaccacgtc ggcgagcgcg
     5161 agcagctcat cgagcggcac gcgcccgggc ccggccgggc ggccgggcag ctgcgcgatg
     5221 cgcacgtcca tgccgaaggc ttcggcgagc cgcgccgtgc cctgaccgag ctcgccccag
     5281 ccgaccacgg ccagcacctt gccggcgagt tcgcggatcg ggtgatcgag caggcagaac
     5341 tgcgcggccc gcggccaggc cccgccggcc aggtcgcgct ggtaggcgag gaagttcgtc
     5401 gacagcgcca gcatcagcag gatcgcgtgc tgcgcgaccg ccggcgtgcc gtagccgcgg
     5461 cagttgacga ccgggatgcc gcgcgcgcgc gccgcttcga gatcgacgtt gttggtgccg
     5521 gtggcggcga cgcagatcag cttcagcgcc gcggccgtgg cgagggtcgc ggcatcgagc
     5581 acgaccttgt tgacgatgac gatgtcggcg ccgcggatgc gcacggcgac ctcgtcgggg
     5641 gcggtggcgg cgtagccggt gaagcgatcg agcgcatggg tcagcgccga gaaatcgaga
     5701 tcgccgagat cgagcgaggc gcggtcgagg aagacgccgt gcatggggcg ggatacctgc
     5761 gggcaacggg gggaacgcgg gcccggcggg gcgccggcaa acccggaata taatcgatgc
     5821 gcccgttccc gcacgctccg aggaagcctc ccgcatgtcc ggaccggact cccgcgcttc
     5881 agcccctgtt cacgtccacg gccgtgccga gacgaccggc atcctgctgg tcaacctcgg
     5941 cacgccggac gccccgacgc cgacggcact gcgccgctat ctcgccgagt tcctgtggga
     6001 ccggcgcgtc gtcgagatcc cgcggccgct gtggtggctg atcctgcacg gcatcatcct
     6061 gcgcacgcgc ccggcgaagt cggcggccaa gtacgcgacg gtctggacgc cggaaggctc
     6121 gccgctgctc gcgatcggcc gccgccagca ggccgggctg caggcgcggc tcgcggccgg
     6181 cgaccatggt ccgctgcagg tcgcgctcgg catgcgctac ggccagccgt cgatcgccac
     6241 ggcgctcgcc gagctgcgcg ccgccggctg cacgcggctg ctggtgctgc cgctgtatcc
     6301 gcagtactcg gccgcgacca cggccacggc cttcgatgcg ctcgccgccg aactcgcgcg
     6361 ctggcgccgg ttgccggaac tgcgctgcgt gatgcagtac cccgacgagc cggcctgggt
     6421 ggacgccgtc gccgcgagcc tcgatcagca cgccgccgcc cacggcggcc tggccgagcg
     6481 cgtgctgttc tcgttccacg g
//
LOCUS       DAOQIE010000232         6496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6402_length_6496_cov_7.265176, whole genome shotgun sequence.
ACCESSION   DAOQIE010000232 DAOQIE010000000
VERSION     DAOQIE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6402_length_6496_cov_7.265176"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..2729
                     /gene="gyrA"
                     /locus_tag="PK144_13535"
     CDS             84..2729
                     /gene="gyrA"
                     /locus_tag="PK144_13535"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNF66795.1"
                     /translation="MVDFAKEVLPVNLEDEMKQSYLDYAMSVIVGRALPDIRDGLKPV
                     HRRVLHAMRELGNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMSRIAHELLADIDKETVDFVPNYDGSETEPAVFP
                     TRVPNLLVNGSAGIAVGMATNIPPHNLGEVVDACIALIDDPTLGIPELMQYLPGPDFP
                     TAAIINGSRGIRDAYMTGRGRIYVRARTEIETDERTGRQTIVVTELPYQVNKARLLEK
                     IAELVREKKLEGISELRDESDKDGMRMVIELKRGEVPEVVLNNLYQHTQMQSVFGINM
                     VALLDGQPRLLNLRQILEAFVRHRREVVTRRTIYELRKARERAHVLEGLSVALANIDA
                     MIELIRRSPSPADAREGLMARVWEAGPVTAMLERAGAVSSRPEGLEAGLGLVGGGYRL
                     SGAQAQAILDLRLHRLTGLEQDRIHGEYQELLARIADLLDILASADRLMQVIRDELVE
                     LRTQYADARRTEIQHTQVDLTLEDLISEEDMVVTLSHAGYAKAQPLTDYRAQRRGGRG
                     KSATAMKDEDFIDKLFIANTHDTLLCFSNRGKVYWIKVYELPQAGRVARGKPLVNLLP
                     MEQGERINAVQPVREFGEDQYLFFATTSGTVKKTPLSEYSRPRAAGIIAIDLRDGDEL
                     VDVAITDGRRDIMLFTDAGKAIRFAETDVRPMGRNAAGVRGIRFKEEESDTDDEAAID
                     RSARVIALVVVGATGDILTVTENGFGKRTAPEEYPLRGRGGQGVISIQTSERNGAVVG
                     AVQVDAADDVMLITDGGTLVRTRVAEISVLSRNTQGVKLIAVQEGERLITVERIETDG
                     EAAEDADSEPEAGDGGDPDSGAGAG"
     gene            2781..3863
                     /gene="serC"
                     /locus_tag="PK144_13540"
     CDS             2781..3863
                     /gene="serC"
                     /locus_tag="PK144_13540"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNF66796.1"
                     /translation="MSRVFNFSAGPAVLPEPVLERARAELSDWHGHGMSVMEMSHRGR
                     DFTAIAEQAEADLRRLLGVPDDYAVLFVQGGASQQFAMVPLNLLPEGGSADYLDTGTW
                     SDKAIEEGARFGRINVVASSRDTRYTTIPAREGWRLDPQAAYVHYTPNETIVGVEFPD
                     VPEVGDRTLVADMSSTILSRPIDVRRFGLIYAGAQKNIGPAGLTVVIVRRELLGRARA
                     QCPTMLDYAVFAKHGSMYNTPPTFGWYLAGLIFEWLLAQGGLPAMAEKNARKAGRLYA
                     YLDGSGFYRNPVDPACRSWMNVPFRLADESLDAVFLKEAEAAGLVGLAGHRIVGGMRA
                     SLYNAMPEAGVEALIAFLQDFVQRRG"
     gene            3890..4978
                     /gene="pheA"
                     /locus_tag="PK144_13545"
     CDS             3890..4978
                     /gene="pheA"
                     /locus_tag="PK144_13545"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNF66797.1"
                     /translation="MTEQEQLLAIRDRIDTIDRQILDLISARARCAQEVAQVKQAHGH
                     GGGNFYRPEREAQVLRRIMELNPGPLSSEEVGRLFREIMSACLALERPLRIAFLGPEG
                     TFTQAAALKHFGHSALTLPQRAIDEVFREVESGDADYGVVPVENSTEGVVNHTLDMFM
                     QSSLNICGEVQLRIHHHLAGFADDPKKLTRIYSHAQSLAQCREWLDANVPGVPRLPVA
                     SNAEAARMARNEPGAGCITGQGALDIYELPVIVANIEDQPDNTTRFLIIGTQPIPPSG
                     DDRTTLLVSSYSRPGSLMALLQPLADHGLSMTRIESRPSRKSMWDYVFFIDFDGHIES
                     PEVVSALADLQQAASLFRVLGSYPKGVL"
     gene            5002..6135
                     /gene="hisC"
                     /locus_tag="PK144_13550"
     CDS             5002..6135
                     /gene="hisC"
                     /locus_tag="PK144_13550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNF66798.1"
                     /translation="MPLPCDFTALAAPGVRGLTPYQPGKPESELKREYGLDSVVKLAS
                     NENPLGPSPKALEAVYACLPELARYPDGNGFELKQALSARLAVDAARITLGNGSNDVL
                     ELLARAFLTPAHNAVMSAHAFAVYPLATLAVGATLKTAPALAADSAMPYGHDLAAMRA
                     LVDDDTRLVFIANPNNPTGTWLAPAELEAFIASLPAQVLCVLDEAYGEYMSPGQLPDS
                     VGWLERYPNLVITRTFSKAYGLAGLRVGYALAHAQVTDLLNRVRQPFNVNLPALTAAT
                     AALGDAAYLAEAVALNRSGLQQWQDGLTALGLGWLPSAGNFLCVDLKGRSGRELFQRL
                     LPKGVILRPVDNYGLPGHIRVTLGTGRENAIALAALGEVLAAG"
     gene            6196..>6496
                     /locus_tag="PK144_13555"
     CDS             6196..>6496
                     /locus_tag="PK144_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNF66799.1"
                     /translation="MIPRLAILGVGLIGGSLARALKRAQRVGEIVGCGRNEDQLRKAV
                     ELGVIDRYTTDPAEAVRGADVVVLGTPVSAMRAVLAAIRDLLAPQAVLTDVGSAKG"
BASE COUNT         1045 a         2313 c         2204 g          934 t
ORIGIN      
        1 ccggggccgg catggtatcc tcgcgcgctt cctgcaggtg cagccccgcg gctgcccgtc
       61 ccccgacaga gctgccccgt aacatggtcg atttcgcgaa ggaagtcctg ccggtcaatc
      121 tcgaagacga gatgaagcag tcgtatctcg attacgcgat gagcgtgatc gtcggccgcg
      181 cgctgcccga catccgcgac ggcctcaagc ccgtgcaccg gcgcgtgctg cacgcgatgc
      241 gcgagctcgg caacgactgg aaccggccgt acaagaagtc ggcgcgcgtc gtcggcgacg
      301 tgatcggcaa gtaccacccg cacggcgaca ccgcggtcta cgacaccatc gtgcgcatgg
      361 cccagccgtt ctcgatgcgc tacatgctgg tcgacggcca gggcaacttc ggctcgatcg
      421 acggcgatgc gccggcggcg atgcgttaca ccgaagtgcg catgagccgc atcgcgcacg
      481 aactgctggc cgacatcgac aaggaaaccg tcgacttcgt gccgaactac gacggctccg
      541 agaccgagcc ggcggtgttc ccgacccgcg tgccgaacct gctggtcaac ggctcggccg
      601 gcatcgcggt cggcatggcg accaacatcc cgccgcacaa cctcggcgag gtcgtcgatg
      661 cctgcatcgc gctgatcgac gacccgacgc tcggcatccc cgagctgatg cagtacctgc
      721 cgggcccgga cttcccgacc gccgcgatca tcaacggctc gcgcggcatc cgcgatgcct
      781 acatgaccgg acgcgggcgc atctacgtgc gggcgcgtac cgaaatcgag accgacgagc
      841 gcaccggccg ccagaccatc gtcgtcaccg agctgccgta tcaggtcaac aaggcgcggc
      901 tgctcgaaaa gatcgccgag ctggtgcgcg agaagaagct cgaaggcatc tcggaactgc
      961 gcgacgagtc cgacaaggac ggcatgcgca tggtcatcga gctgaagcgc ggcgaagtgc
     1021 ccgaggtcgt gctgaacaac ctgtatcagc acacgcagat gcagagcgtg ttcggcatca
     1081 acatggtcgc gctgctcgac ggccagccgc ggctgctgaa cctccggcag atcctcgaag
     1141 ccttcgtgcg tcaccggcgc gaagtcgtca cgcggcgcac gatctacgag ctgcgcaagg
     1201 cccgcgaacg cgcccacgtg ctcgaaggcc tgtcggtggc gctcgccaac atcgacgcga
     1261 tgatcgagct gatccgccgc tcgccgagtc cggcggatgc cagggaaggg ctgatggcgc
     1321 gcgtctggga ggccggaccg gtcacggcga tgctggagcg cgccggggcg gtgtcctcgc
     1381 gtcccgaagg cctcgaagcc gggctcgggc tggtcggggg cggctaccgg ctctcgggcg
     1441 cgcaggcgca ggcgatcctc gacctgcgcc tgcaccggct gaccgggctg gagcaggacc
     1501 gcatccacgg cgaataccag gagctgctcg cgcggatcgc cgacctgctc gacatcctcg
     1561 cgagtgctga ccggctgatg caggtgatcc gcgacgaact cgtcgagctg cgcacgcagt
     1621 acgccgatgc gcggcgcacc gagatccagc acacgcaggt cgacctgacg ctggaggatc
     1681 tgatctccga ggaggacatg gtcgtcacgc tgtcgcacgc cggctacgcc aaggcgcagc
     1741 cgctgaccga ctaccgtgcg cagcgccgcg gcgggcgcgg caagtcggcg acggcgatga
     1801 aggacgagga cttcatcgac aagctgttca tcgccaacac ccacgacacg ctgctctgct
     1861 tctcgaaccg cggcaaggtg tactggatca aggtctacga gctgccgcag gccggacgcg
     1921 tcgcgcgcgg caagccgctc gtgaacctgc tgccgatgga gcagggcgag cgcatcaacg
     1981 ccgtgcagcc ggtgcgcgag ttcggcgagg accagtacct gttcttcgcg accacctccg
     2041 gcacggtcaa gaagacgccg ctgtccgaat actcgcgccc gcgcgccgcc ggcatcatcg
     2101 cgatcgacct gcgcgacggc gacgaactgg tggacgtggc gatcaccgac ggccggcgcg
     2161 acatcatgct gttcaccgac gctggcaagg cgatccgctt cgccgagacc gacgtgcggc
     2221 cgatgggccg caacgccgcc ggcgtgcgcg gcatccgctt caaggaagag gaatccgaca
     2281 ccgacgacga ggccgcgatc gaccgcagcg cgcgcgtgat cgcgctggtg gtggtcggtg
     2341 ccaccggcga catcctgacc gtcaccgaga acggcttcgg caagcgcacc gcgcccgagg
     2401 aatacccgct gcgcggccgc ggcggccagg gcgtgatctc gatccagacc agcgagcgca
     2461 acggcgcggt ggtcggtgcg gtgcaggtcg atgccgccga tgacgtgatg ctgatcaccg
     2521 acggcggcac gctggtgcgc acgcgcgtcg ccgagatctc ggtgctgtcg cgcaacaccc
     2581 agggcgtgaa gctgatcgcg gtgcaggaag gcgagcggct gatcacggtc gagcgcatcg
     2641 agaccgacgg cgaggccgcc gaggatgccg acagcgagcc cgaggccggt gacggcggcg
     2701 accccgattc cggtgccggt gccggctgag gccggcggca cgggcaggtc cgatttttcc
     2761 tctgttcgag gcgagttccg atgtcgagag tgttcaattt cagcgccggc cccgcggtgc
     2821 tgcccgagcc ggtgctcgaa cgtgcgcgcg cggagctcag cgactggcac ggccacggca
     2881 tgtcggtcat ggagatgtcg caccgcggcc gtgacttcac cgccatcgcc gaacaggccg
     2941 aggccgatct gcgccggctg ctcggcgtgc cggacgacta cgcggtgctg ttcgtgcagg
     3001 gcggggcctc gcagcagttc gcgatggtgc cgctgaacct gctgcccgag ggcggctcgg
     3061 ccgattacct cgataccggc acctggtcgg acaaggcgat cgaggagggc gcgcgcttcg
     3121 ggcgcatcaa cgtcgtcgcc agcagccgcg acacgcgcta cacgacgatc ccggcgcgcg
     3181 agggctggcg gctcgatccg caggccgcct atgtgcatta cacgccgaac gagaccatcg
     3241 tcggcgtcga gttcccggac gtgcccgagg tcggcgaccg gacgctcgtt gccgacatgt
     3301 cctcgacgat cctgtcgcgc cccatcgacg tgcggcgctt cgggctgatc tacgccggtg
     3361 cgcagaagaa catcggcccg gccgggctga ccgtcgtcat cgtgcgccgc gaactgctcg
     3421 gtcgcgcccg cgcgcagtgc ccgacgatgc tcgactatgc ggtgttcgcg aagcacggct
     3481 cgatgtacaa cacgccgccg accttcggct ggtatctcgc cggactgatc ttcgaatggc
     3541 tgctcgcgca gggcggcctg ccggcgatgg ccgaaaagaa cgcccgcaag gccggcaggc
     3601 tctacgccta cctcgatggc tccggcttct accgcaaccc ggtcgatccg gcctgccgct
     3661 cgtggatgaa cgtgccgttc cggctcgccg acgagtcgct cgatgccgtg ttcctgaagg
     3721 aagccgaggc cgccggactg gtcgggctgg cagggcatcg catcgtcggc ggcatgcgcg
     3781 cgagcctcta caacgccatg cccgaggccg gcgtcgaggc gctgatcgcg ttcctgcagg
     3841 acttcgtgca gcgccggggc tgaggcccgg catcaccggt acccgaacga tgaccgaaca
     3901 ggaacagctg ctggcgatcc gtgaccgcat cgacacgatc gaccgccaga tcctcgatct
     3961 gatcagtgcc cgcgcgcgct gcgcgcagga agtcgcccag gtcaagcagg cgcacggtca
     4021 cggcggcggc aatttctatc gccccgagcg cgaggcgcag gtgctgcgcc ggatcatgga
     4081 gctgaacccc ggcccgctgt cgagcgagga ggtcggccgg ctgttccgcg agatcatgtc
     4141 cgcgtgcctg gcgctggagc ggccgctgcg catcgccttc ctcggccccg agggcacctt
     4201 cacgcaggcg gcggcactca agcacttcgg ccacagcgcg ctgaccctgc cgcagcgcgc
     4261 gatcgacgag gtgttccgcg aggtcgaatc cggcgatgcc gactacggcg tcgtgccggt
     4321 cgagaactcg accgagggcg tcgtcaacca cacgctcgac atgttcatgc agtcctcgct
     4381 gaacatctgc ggcgaggtgc agctgcgcat ccatcatcat ctggccggct tcgccgacga
     4441 tccgaagaag ctcacgcgca tctactcgca tgcgcagtcg ctcgcgcagt gccgcgaatg
     4501 gctcgatgcc aacgtgcccg gcgtgccgcg cctgccggtg gcctccaacg ccgaggccgc
     4561 gcgcatggcg cgcaacgaac ccggcgccgg ctgcatcacc ggccagggcg cgctcgacat
     4621 ctacgagctg ccggtgatcg tcgccaacat cgaggaccag ccggacaaca ccacgcgctt
     4681 cctgatcatc ggcacgcagc cgatcccgcc gtccggcgat gaccgcacga cgctgctggt
     4741 gtcctcgtac agccggcccg gctcgctgat ggcgctgctg cagccgctcg ccgaccacgg
     4801 cctgtcgatg acgcgcatcg agtcgcgccc ctcgcgcaag agcatgtggg actacgtgtt
     4861 cttcatcgac ttcgacggcc acatcgagtc gccggaggtc gtcagcgcgc tcgccgacct
     4921 gcagcaggcg gcgagcctgt tccgcgtgct cggttcctac ccgaaaggcg tgctgtaaaa
     4981 ccccggatgc cggagacccc gatgccgcta ccctgcgatt tcaccgccct ggccgccccc
     5041 ggcgtgcgcg gcctgacccc gtaccagccc ggcaagcccg aaagcgagct gaagcgcgag
     5101 tacgggctgg attcagtcgt caagctggcc tcgaacgaga accccctcgg cccgagcccg
     5161 aaggcgctcg aagccgtgta tgcctgcctg ccggaactcg cgcgctaccc cgacggcaac
     5221 ggcttcgagc tgaagcaggc gctgtcggcg cgcctcgcgg tcgatgcggc gcgcatcacg
     5281 ctcggcaacg gctcgaacga cgtgctcgaa ctgctggcgc gcgccttcct gacgccggcg
     5341 cacaacgccg tgatgtcggc gcatgcgttc gcggtctacc cgctggcgac gctcgcggtc
     5401 ggcgcgacgc tgaagaccgc cccggcgctc gccgccgaca gtgcgatgcc ctacggccat
     5461 gatctggccg cgatgcgcgc cctcgtcgat gacgacacgc gcctggtgtt catcgccaac
     5521 ccgaacaacc cgaccggcac ctggctcgcg ccggccgaac tcgaagcctt catcgccagc
     5581 ctgccggcgc aggtgctctg cgtgctcgac gaggcctatg gcgagtacat gagccccgga
     5641 cagctgccgg actcggtcgg ctggctggag cgctacccga acctcgtcat cacgcgcacc
     5701 ttctcgaagg cctacggcct cgccggcctg cgcgtcggct acgcgctcgc gcatgcgcag
     5761 gtcaccgacc tgctgaaccg cgtgcgccag cccttcaacg tcaacctgcc ggcgctgacg
     5821 gcggcgacgg ccgcgctcgg cgacgccgcc tacctcgccg aggccgtggc gctgaaccgc
     5881 agcggtctgc agcagtggca ggacggactc acggcgctcg gcctcggctg gctgccctcg
     5941 gccggcaact tcctgtgcgt cgacctcaag ggccgcagcg gccgcgagct gttccagcgc
     6001 ctgctgccga agggcgtgat cctgcgaccg gtcgacaact acggcctgcc cggccatatc
     6061 cgcgtcacgc tcggcaccgg acgcgagaac gccatcgccc tcgccgcgct cggcgaggtg
     6121 ctggcggcgg gctgaagccg ccgcccgatg cccaccgtgc catcgtcagg gagattgccc
     6181 gtgagccacg acatcctgat cccgcgcctc gccatcctcg gtgtcggcct catcggcggt
     6241 tcgctggcgc gggcgctgaa gcgcgcgcag cgcgtcggcg agatcgtcgg ctgcgggcgc
     6301 aacgaggatc agctcaggaa ggccgtcgag ctcggcgtca tcgaccgcta cacgaccgac
     6361 ccggccgagg ccgtgcgcgg ggccgacgtg gtcgtgctcg gcacgccggt gtcggcgatg
     6421 cgggcggtgc tggcggcgat ccgcgacctg ctggcgccgc aggccgtgct gaccgatgtc
     6481 ggtagcgcca agggct
//
LOCUS       DAOQIE010000233         6458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6462_length_6458_cov_7.962518, whole genome shotgun sequence.
ACCESSION   DAOQIE010000233 DAOQIE010000000
VERSION     DAOQIE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6462_length_6458_cov_7.962518"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..3104
                     /locus_tag="PK144_13560"
     CDS             3..3104
                     /locus_tag="PK144_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNF66800.1"
                     /translation="MLRPVATTLLMVAILLSGLLAYRLLPVSALPDVQYPTIQVATFY
                     PGASPDVIASSVTAPLERQFGQMPGLKRMSSTSAAGASVITLQFDLDLSMDVAEQQVQ
                     ASINAGSNLLPADLPAPPVYSKVNPADAPVLTLGLTSATLPLVQVQDLADTRLAQKLS
                     QVPGVGLVTLAGGQRPALRIRANPSALAAYGIGLDTLRTAVAAANVNQAKGSFDGPAR
                     AYTIDANDQLRDAADYRALVIAYRNGAPVRLRDLAEVAEDAENTKLAAWTGSGGGAVQ
                     PAIVVNVLRQPGANVIEVVDRIRALLPGLQAALPATIDVAVLSDRTVTIRASVEDVQF
                     ELAWAIALVILVIYLFLRSARATLIPALAVPLSLVGTLGVMYFAGFSINNLTLMAFTI
                     ATGFVVDDAIVMLENIARYVEAGEKPLAAALKGAQEIGFTIVSLTVSLIAVLIPLLFM
                     GDVVGRLFREFAVTLAVTILISAVVSLTLTPMLCARLLRSGHGDGAGAGRFHRAAGRV
                     IDATIAHYDRALARVLDHQPLVLLVTLATVALTAWLYVMMPKGFFPVQDTGLLQGISQ
                     APASISFAAMAERQQALARVLLADPAIEAVSTYIGVDGTNATPNTGRFSVTLKPLAAR
                     GHVSAAGVIARLEAPLAQVAGIRLYLQPVQDLAIEDRMSRSRYQFTVEDPDGARVAAA
                     VEQLVERLSASAQLRNVASEQQDGGLAAYVRIDRDSAARLGVAIADVDNALYDAFGQR
                     LISTVYTQSSQYRVVLELGADWRRGPRALDALYVSSANGVQVPLTALASIEERTLPLA
                     LLRVGQFPATTVSFDLAPGASLGAAVAGIEAVTQALALPASTRTSFEGAASAFRAALD
                     NQLWLLLAAVVTVYIVLGVLYESFVHPVTILSTLPPAGIGALLALELAGQDLGVIAII
                     GIVLLIGIVKKNAIMMIDFALEAERGEGLTPRAAIHRACLLRFRPILMTTLAALFGAL
                     PMVLGSGTGSELRQPLGLAIVGGLIVSQLLTLFTTPVVYLAFERLARRLRGGDAAAAG
                     GPA"
     gene            3115..6234
                     /locus_tag="PK144_13565"
     CDS             3115..6234
                     /locus_tag="PK144_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF66801.1"
                     /translation="MNLSEPFIRRPVATILLTLGLALAGLVAYRLLPVSALPQIDVPT
                     IAVSASLPGASAETMAATVATPLERRLGRIAGVTEMTSSSSLGSTSVTLQFDLARDIS
                     SAANDVQAAINAARADLPAMPRNPTWRKLNPSDAPVMILALASDTYDRGRLYDWASSV
                     LAQKISQVAGVGQVTVGGAALPAVRVELNPDALARYGIDPNAVATAIAGANPRRPLGM
                     VEDDSRRWQILANDRASSADEYRGLVVAWRNGAAVRLADVASVIDSVEDVRNSGSANG
                     RPSVSLIVRREPGANVIDTVEGIRAVLPQLRATLPAAVDFDVMQDRSTTVRASLDEVE
                     LTLAIAIGLVVLVVYAFLRDLRATLIPGIAVPVSLLGTGCVMWLCGYSLNNLSLMALT
                     IATGFVVDDAVVVVENTVRHVESGQPVRAAALRGAREVGFTVLAMSVSLVAVFIPVLL
                     MGGLPGRLLREFGVTLSAAVLISLVISLTTTPMLCAHWLRAPPAGPRESHGRLWQAFD
                     RGLRHLNRAYARSLAVVLRHAAVVLMLLAAVIALNVWLYVQVPKGLFPQQDTGRLSGS
                     VRADQAISFQAMRGKLDAFVAIVREDPAVRTVVASTGGGSFGAQRNSGQMFIELKPLE
                     ERGVSADQVIARLRGRTAHIPGAQLFLQSVQDIRTGGRSSNAQFQYTLQADSLDGLRR
                     WTGPVTRALAGLPQLADVNSDREERGLQLTLNVDRDAAARLGVSMQAIDRTLYAAFGQ
                     RQVSTIYAALNQYRVVMEVAPQYWQRPETLARLYVTADSGAQVPLASLASWGPTPGPL
                     SVNHQGVFVATTVSFNLAAGVAIGEAVAAINAAVARLGLPESVHGSFEGSARAYAEGV
                     GGQPLLILAALIAVYIVLGMLYESWVHPLTILSTLPSAGVGALIVLMATGTEFGIMAL
                     IGVILLIGIVKKNAIMMIDVALDLERRHGVSPLVAIHAAARLRLRPILMTTLAALLGA
                     LPLAFGSGLGAELRRPLGLAIVGGLFVSQWLTLYTTPVVYLVLDRLGRRLRRERSPAR
                     SAIHAPLPD"
BASE COUNT          798 a         2345 c         2316 g          999 t
ORIGIN      
        1 tcatcctacg cccggtcgcg accacgctgc tgatggtggc gatcctgctg tcggggctgc
       61 tcgcgtaccg gctgctgccg gtctcggcgc tgcccgacgt gcagtacccg accatccagg
      121 tcgcgacctt ctatcccggt gccagcccgg acgtgatcgc ctcctcggtc acggcgccgc
      181 tggagcgcca gttcgggcag atgccgggac tcaagcgcat gtcctcgacc agtgccgccg
      241 gcgcgtcggt gatcacgctg cagttcgacc tcgacctgtc gatggacgtg gccgagcagc
      301 aggtgcaggc ctcgatcaac gccggctcca acctgctgcc ggccgacctg ccggcgccgc
      361 cggtgtacag caaggtcaat ccggccgatg cgccggtgct gacgctcgga ctcacgtcgg
      421 cgacgctgcc gctggtgcag gtgcaggatc tcgccgacac gcggctcgcg cagaagctgt
      481 cgcaggtgcc gggcgtcggc ctcgtgacgc tcgccggcgg ccagcgcccg gcgctgcgca
      541 tccgcgccaa cccctcggcg ctcgccgcct acggcatcgg cctcgacacg ctgcgcacgg
      601 ccgtggccgc ggccaacgtc aatcaggcca agggcagctt cgacgggccg gcgcgcgcgt
      661 acacgatcga cgccaacgac cagctgcgcg acgccgccga ctaccgcgcg ctggtgatcg
      721 cgtacagaaa cggcgcgccg gtgcgcctgc gcgatctggc cgaggtcgcc gaggacgccg
      781 agaacacgaa gctcgcggcg tggaccggca gcggcggcgg ggccgtgcag ccggcgatcg
      841 tcgtcaacgt gctgcgccag cccggggcca acgtcatcga ggtcgtcgac cgcatccgcg
      901 cgctgctgcc ggggctgcag gccgcgctgc cggcgaccat cgatgtcgcc gtgctcagcg
      961 accgcacggt gacgatccgc gcctcggtcg aggacgtgca gttcgagctg gcctgggcga
     1021 tcgcgctggt gatcctggtg atctacctgt tcctgcgcag cgcccgcgcg acgctgatcc
     1081 cggcgctcgc cgtgccgctg tcgctggtcg gcacgctcgg cgtcatgtac ttcgccggct
     1141 tcagcatcaa caacctgacg ctgatggcct tcacgatcgc gaccggcttc gtcgtcgacg
     1201 atgcgatcgt catgctcgaa aacatcgccc gctacgtcga ggccggcgag aagccgctcg
     1261 ccgcggcgct gaagggcgcg caggagatcg gcttcacgat cgtgtcgctg accgtgtcgc
     1321 tgatcgcggt gctgatcccg ctgctgttca tgggcgatgt ggtcgggcgg ctgttccgcg
     1381 agttcgcggt gacgctggcc gtgaccatcc tgatctcggc cgtggtgtcg ctgacgctga
     1441 cgccgatgct gtgcgcgcgg ctgctgcgct ccggtcacgg cgatggcgcc ggtgccgggc
     1501 gcttccaccg cgccgccggc cgcgtcatcg atgcgacgat tgcgcactac gaccgggcgc
     1561 tcgcgcgtgt gctcgaccac cagccgctgg tgctgctggt gacgctcgcg accgtggcgc
     1621 tgaccgcctg gctgtacgtg atgatgccga agggcttctt cccggtgcag gacaccggtc
     1681 tgctgcaggg catctcgcaa gcgccggcca gcatctcgtt cgcggcgatg gccgagcgcc
     1741 agcaggcgct ggcgcgcgtg ctgctcgccg atccggcgat cgaggcggtg tcgacctaca
     1801 tcggcgtcga cggcaccaac gccacgccga acaccggccg cttctcggtg acgctgaagc
     1861 cgctggcggc gcgcggccat gtatcggcgg ccggcgtgat cgcgcggctc gaagcgccgc
     1921 tcgcgcaggt ggcgggcatc cgcctgtacc tgcagccggt gcaggatctc gcgatcgagg
     1981 accgcatgag ccgcagccgc taccagttca cggtcgagga cccggacggc gcgcgcgtcg
     2041 ccgcggccgt ggagcagctg gtcgagcggc tctcggcctc ggcgcagctt cgcaacgtcg
     2101 cgagcgagca gcaggacggc ggactcgctg cttacgtgcg catcgaccgc gacagcgccg
     2161 cgcgcctcgg cgtggcgatc gccgatgtcg acaacgcgct ctacgacgcc ttcggccagc
     2221 ggctgatctc gaccgtgtac acgcagtcca gccagtaccg cgtcgtgctc gaactcgggg
     2281 ccgactggcg gcgcgggccg cgggcgctcg atgcgctgta cgtgagctcc gcgaacggcg
     2341 tgcaggtgcc gctgacggcg ctcgcgagca tcgaggagcg cacgctgccg ctcgcgctgc
     2401 tgcgcgtcgg gcagtttccg gcgacgacgg tgtccttcga tctggcgccg ggcgcgtcgc
     2461 tcggcgcggc ggtcgccggc atcgaggcgg tgacgcaggc gctcgcgctg ccggcgagca
     2521 cgcgcacgag cttcgagggc gcggccagcg cgtttcgcgc cgcgctcgac aaccagctct
     2581 ggctgctgct cgcggcggtc gtgaccgtct acatcgtgct cggcgtgctc tacgagagct
     2641 tcgtgcaccc ggtgacgatc ctctcgacgc tgccgccggc cggcatcggc gcgctgctgg
     2701 cgctggagct cgccgggcag gatctcggcg tcatcgcgat catcggcatc gtgctgctga
     2761 tcggcatcgt caagaagaac gcgatcatga tgatcgactt cgcgctggag gccgaacgcg
     2821 gcgagggcct cacgccgcgc gcggcgatcc accgcgcctg cctgctgcgc ttccggccga
     2881 tcctgatgac gacgctggcg gcgctgttcg gcgcgctgcc gatggtgctc ggcagcggca
     2941 ccggctcgga gctgcgccag ccgctcgggc tcgcgatcgt cggcgggctg atcgtcagcc
     3001 agttgctgac gctgttcacg acgccggtcg tgtatctcgc cttcgagcgg ctggcgcgcc
     3061 ggctgcgcgg cggcgatgcg gccgcggccg gcggcccggc ctgaggctgc gccggtgaac
     3121 ctgtcggagc cgttcatccg ccggccggtc gcgaccatcc tgctgaccct cggcctggcg
     3181 ctcgccggcc tcgtcgccta ccggctgctg ccggtgtcgg cgctgccgca gatcgacgtg
     3241 ccgaccattg ccgtcagcgc cagcctgccg ggggcgagcg ccgagaccat ggcggcgacg
     3301 gtcgcgaccc cgctcgaacg ccggctcggg cgcatcgccg gcgtcaccga gatgacctcg
     3361 tcgagcagcc tcggcagcac cagcgtcacg ctgcagttcg atctggcccg cgacatcagc
     3421 agtgccgcca atgacgtgca ggcggcgatc aatgccgcgc gcgccgacct gccggcgatg
     3481 ccgcgcaacc ccacctggcg caagctcaac ccttcggatg cgccggtgat gatcctcgcg
     3541 ctcgcctccg acacctacga ccgcggccgg ctgtacgact gggcctcgtc ggtgctggcg
     3601 cagaagatct cgcaggtcgc cggcgtcggt caggtcacgg tcggcggtgc ggcgctgccg
     3661 gcggtgcgcg tggagctgaa ccccgatgcg ctggcgcgct acggcatcga cccgaacgcg
     3721 gtcgccacgg cgatcgccgg tgccaacccg cgccggccgc tcggcatggt cgaggacgac
     3781 tcgcgccgct ggcagatcct cgccaacgat cgcgccagca gcgccgacga ataccgcggg
     3841 ctggtggtgg cctggcgcaa cggcgccgcg gtgcgcctcg ccgatgtcgc gagcgtcatc
     3901 gattcggtcg aggacgtgcg caacagcggt tcggccaacg gccggccctc ggtgtcgctg
     3961 atcgtgcgcc gcgagcccgg ggccaacgtc atcgacacgg tcgagggcat ccgcgccgtg
     4021 ctgccgcagc tgcgcgcgac gctgccggcg gcggtcgatt tcgacgtgat gcaggaccgc
     4081 tcgaccacgg tgcgcgcctc gctcgacgag gtcgagctga cgctggcgat cgcgatcggg
     4141 ctggtcgtgc tggtcgtcta cgccttcctg cgcgacctgc gcgcgaccct gatccccggc
     4201 atcgccgtgc cggtgtcgct gctcggcacc ggctgcgtga tgtggctgtg cggctacagc
     4261 ctgaacaacc tgtcgctgat ggcgctgacc atcgccaccg gcttcgtcgt cgacgatgcg
     4321 gtcgtcgtcg tcgagaacac cgtgcgccac gtcgaatccg gccagccggt gcgcgccgcg
     4381 gcgctgcgcg gggcgcgcga ggtcggcttc acggtgctgg cgatgagcgt gtcgctggtg
     4441 gcggtgttca tcccggtact gctgatgggc gggctgccgg ggcggctgct gcgcgagttc
     4501 ggcgtgacgc tgtcggcggc ggtgctgatc tcgctcgtga tctcgctgac gacgacgccg
     4561 atgctgtgcg cgcactggct gcgcgcgccg ccggccgggc cgcgcgagtc gcacggccgg
     4621 ctgtggcagg ccttcgaccg ggggcttcgg cacctcaacc gcgcctatgc gcgcagcctc
     4681 gccgtcgtgc tgcgccacgc cgccgtcgtg ctgatgctgc tcgcggcggt gatcgcgctg
     4741 aacgtctggc tgtacgtgca ggtgccgaag ggcctgttcc cgcagcagga caccgggcgc
     4801 ctgagcggct cggtgcgcgc cgatcaggcg atctcgttcc aggcgatgcg cggcaagctc
     4861 gatgccttcg tcgcgatcgt gcgcgaagac ccggcggtgc gcaccgtcgt cgcctcgacc
     4921 ggtggcggct ccttcggcgc gcagcgcaac agcggccaga tgttcatcga gctgaagccg
     4981 ctggaggagc gcggcgtcag tgccgatcag gtcatcgcgc ggctgcgcgg gcggacggcg
     5041 cacatccccg gcgcgcagct gttcctgcag tcggtgcagg acatccgcac cggcgggcgt
     5101 tcgtcgaacg cgcagttcca gtacacgctg caggccgaca gcctcgacgg cctgcgccgc
     5161 tggaccgggc cggtcacgcg cgcgctcgcc ggcctgccgc agctcgccga cgtgaacagc
     5221 gaccgcgagg agcgcggcct gcagctgacg ctgaacgtcg atcgcgacgc tgccgcgcgc
     5281 ctcggcgtca gcatgcaggc gatcgaccgc acgctgtacg cggccttcgg ccagcggcag
     5341 gtgtcgacga tctacgcggc gctgaaccag taccgcgtgg tcatggaggt cgccccgcag
     5401 tactggcagc ggcctgaaac actggcgcgt ttgtacgtca ccgcggattc cggcgcgcag
     5461 gtgccgctgg cgagccttgc gagctggggg ccgacgccgg ggccgctgag cgtcaatcac
     5521 cagggcgttt tcgttgcgac cacggtgtcg ttcaacctgg ccgcgggcgt cgcgatcggc
     5581 gaggcggtcg ccgcgatcaa cgccgcggtc gcgcgcctcg gcctgcccga gagcgtgcac
     5641 ggcagcttcg aaggctcggc gcgcgcctac gccgagggcg tcggcggcca gccgctgctg
     5701 atcctcgccg cgctcatcgc cgtctacatc gtgctcggca tgctctacga gagctgggtg
     5761 cacccgctga ccatcctgtc gacgctgccc tcggccggcg tcggcgcact gatcgtgctg
     5821 atggcgaccg gcaccgagtt cgggatcatg gcgctgatcg gcgtgatcct gctgatcggc
     5881 atcgtcaaga agaacgcgat catgatgatc gacgtggcgc tcgacctcga acgtcgccac
     5941 ggcgtctcgc cgctggtcgc gatccacgcc gcggcgcggc tgcggctgcg cccgatcctg
     6001 atgaccacac tcgcggcgct gctcggcgcg ctgccgctcg ccttcggcag cggcctcggc
     6061 gccgaactgc gccggccgct gggcctggcc atcgtcggcg gcctgttcgt cagccagtgg
     6121 ctgacgctgt acacgacgcc ggtcgtctac ctcgtgctcg accgtctcgg ccgccgcctg
     6181 cgccgcgagc gcagccctgc ccggagcgcc atccatgccc cgctgcccga ctgaagcccg
     6241 ctgccgtgcc tcacgctttc cgtcgggtgc cggctcctgc ccgcaggcag ccctcgctgc
     6301 ggacggtgaa cgaggggagg cgggatcagg ccgccaaagc ggcgccgcag gctttgcggt
     6361 cgccgccctg cgctgtctgg ccgtcgccgt gctcgcgacc ggcctcgccg cctgcacggt
     6421 cggccccgac tacgcgcgcc cggctgcgcc ggtgccgg
//
LOCUS       DAOQIE010000234         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6471_length_6453_cov_7.913254, whole genome shotgun sequence.
ACCESSION   DAOQIE010000234 DAOQIE010000000
VERSION     DAOQIE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6471_length_6453_cov_7.913254"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /gene="lpdA"
                     /locus_tag="PK144_13570"
     CDS             <1..1697
                     /gene="lpdA"
                     /locus_tag="PK144_13570"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF66802.1"
                     /translation="KVDQTLIVLESDKASMDVPSTAAGTIVELKVKVGDKVSEGAVIA
                     IVEAGAAAEAAPAPAPAAAPAAAAPAPAPVAAPLPAGSYSGPVDVECEMLVLGAGPGG
                     YSAAFRAADLGLKTVLVERYATLGGVCLNVGCIPSKALLHTAAVIDESAALAHHGVVF
                     QAPTIDIDKLRGFKESVIKKLTGGLAGMAKARKVEIVRGYGAFLDANHVEVEVTEGSG
                     QDKTGAKKVVRFQKCIIAAGSQAVRLPFMPEDPRVIDSTGALELKGAPKRLLVIGGGI
                     IGLEMATVYSALGTSIDVVEMLDGVMAGADRDLVKVWEKKNAHRFDRIMLKTKTVSAE
                     AKADGIWVKFEGEKAPADAQCYDLVLVAVGRSPNGNKIAAAKAGVAVTDRGFIPVDKQ
                     MRTNVAHIFAIGDIVGQPMLAHKAVHEAHVAAENAAGHKAFFDARQIPSVAYTDPEVA
                     WAGKTEDQCKAEGIDYGKAVFPWAASGRAIANGRDEGFTKLLFDKATHRIVGGAIVGT
                     HAGDLIGEICLAVEMGADPVDIGKTIHPHPTLCESIGMAAEVFEGVCTDLPPQKKK"
     gene            2150..2551
                     /locus_tag="PK144_13575"
     CDS             2150..2551
                     /locus_tag="PK144_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010795999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNF66803.1"
                     /translation="MKTKAVLTLTEVRSILDAAEQHARAQGWAVSIAVVDDGGHALGL
                     LRLDDASPVSAEIALGKARTAALGRRDSEGYEDMINQGRTAFVSAPLPVMLTGGVVLR
                     IDGQVIGAVGVSGVRPEQDAETARAGIAAVV"
     gene            complement(2565..3539)
                     /locus_tag="PK144_13580"
     CDS             complement(2565..3539)
                     /locus_tag="PK144_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66804.1"
                     /translation="MIVPAYWAEARRQQRKEGRQITVRRYGWSDASQADAQQHAETRA
                     GEALQRLLAGERLDRREPKQPYNGADGLPIREEIVERHGTAVVTRNSYGARCLNVPDV
                     LFADIDHAHRAPLRLSVIVFVLAALAAALAGHACGSGFGYALAFAAVLGCAPAAHGLF
                     RLWRWLAGGAEAQALRRVRRYAHRHPDWGIRVYRTPAGLRLLVTHRPFAPGATEVAAF
                     FAAVGTDPLYARMCRNQRCFRARVSAKPWRIGIGAHLKPRPGVWPVAAEKQPQRTAWI
                     AAYERAAEAWAACTSLEHLGPAAIHPAVAPVLALHDRLSRAGSKLPIA"
     gene            complement(3552..3920)
                     /locus_tag="PK144_13585"
     CDS             complement(3552..3920)
                     /locus_tag="PK144_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF66805.1"
                     /translation="MTEPSTATPAPASDPLPAGDPGPDSSPCTQCGACCATYRVSFYW
                     GEAPQLGLPGSLYEALSAWHACMRGTASAPMRCVALAGGIGQSVACTVYAQRPSPCRE
                     LQAGDDRCQRARARHGLAAL"
     gene            4159..5364
                     /locus_tag="PK144_13590"
     CDS             4159..5364
                     /locus_tag="PK144_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HNF66806.1"
                     /translation="MKPLYDVVSQGLIHSWKPVKAAKAGRAYRCRCSRPVFFRNSECL
                     ACHAPLGFEPLLGELGALQPGDAEGLWRLENGDGAQYRRCANFEQIGCNWLIPADSDA
                     TLCLSCVLNRTIPDLSEPENLAYWQSIEIAKRQLVAQLLALGLPVRSKVSDDPDNGLC
                     FDFLKSLPDGPRVLTGHDEGLITLNVEEADDARREAMRKQLGEPYRTLLGHLRHEVGH
                     YYWDVLVRDSHWLEPYRALFGDESFDYATALQQHYENGPPADWPQQFVSAYATSHPWE
                     DWAETWAHYLHLVDTMNTALAFGLNAEDVEVDTEPFGSDALYDPQHPGAGQFLYFINA
                     WVDLVTILNELSRSMGQRDFYPFVMSRPVVAKLHFIHLVIEDARDQHLQAQDGGVEAA
                     TTMAEPVVS"
     gene            complement(5405..6082)
                     /locus_tag="PK144_13595"
     CDS             complement(5405..6082)
                     /locus_tag="PK144_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNF66807.1"
                     /translation="MIQVIIIAEGQTEETFVRDVMAPVFAASGIILTARLVEVAGGGR
                     GGALSYERVWKFLRNTLRERASTYVSTFFDLHKLDTAFPAHAGSLKISDSGRRAGYLE
                     TAFHADVIARTGCRPDRFIPHIQPWEFETLLFSDLTTLTGTETSWARHLPELQKIRSG
                     FDHPEQINGGQDTKPSARLGLLRNPGFRKVRHGPLAAGRIGLQRIEAECPHFAAWLQR
                     LRTLTPL"
     gene            complement(6079..>6453)
                     /locus_tag="PK144_13600"
     CDS             complement(6079..>6453)
                     /locus_tag="PK144_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF66808.1"
                     /translation="PLKAHMLSDGTLRFICLTTLLLQPTELLPDTILIDEPELGLHPY
                     AIALLADMLREVSENTQLIISTQSVELLNHFNAEDVIVVDRINDESVFRRLDTESLHD
                     WLQDYSLGELWKQNVLGGRPSR"
BASE COUNT         1046 a         2162 c         2240 g         1005 t
ORIGIN      
        1 tcaaggtcga ccagaccctg atcgtgctcg aatccgacaa ggcgagcatg gacgtgccct
       61 cgaccgcggc cggcaccatc gtcgagctga aggtcaaggt cggcgacaag gtcagcgaag
      121 gcgcggtcat cgccatcgtc gaggccggcg ctgccgccga ggccgccccg gctccggccc
      181 cggcggctgc ccccgccgcg gctgcccccg ctccggcgcc ggttgccgcg ccgctgccgg
      241 ccggcagcta cagcggtccg gtcgatgtcg agtgcgagat gctcgtgctc ggcgccggcc
      301 ccggcggcta ttcggcggcg ttccgcgcgg ccgacctcgg cctgaagacg gtgctggtcg
      361 agcgctacgc cacgctcggc ggcgtctgcc tgaacgtcgg ctgcatcccg tcgaaggcgc
      421 tgctgcacac cgccgccgtc atcgacgagt cggccgcgct cgcgcaccac ggcgtggtgt
      481 tccaggcgcc gaccatcgac atcgacaagc tgcgcggctt caaggaaagc gtcatcaaga
      541 agctgaccgg cggcctcgcc ggcatggcca aggcgcgcaa ggtcgagatc gtgcgcggct
      601 acggtgcctt cctcgacgcc aaccacgtcg aggtcgaagt caccgagggc agcggtcagg
      661 acaagaccgg cgccaagaag gtcgtgcgct tccagaagtg catcatcgcc gccggctcgc
      721 aggccgtgcg cctgccgttc atgcccgagg acccgcgcgt catcgactcg accggcgcgc
      781 tggagctgaa gggcgcgccg aagcgcctgc tcgtcatcgg cggcggcatc atcggtctgg
      841 aaatggcgac cgtgtactcg gccctcggca catcgatcga cgtggtcgag atgctcgacg
      901 gcgtcatggc cggtgccgac cgtgatctgg tcaaggtgtg ggagaagaag aacgcccacc
      961 gcttcgacag gatcatgctg aagacgaaga ccgtctcggc cgaagccaaa gccgatggca
     1021 tctgggtgaa gttcgaaggc gagaaggctc cggccgacgc gcagtgctac gacctggtgc
     1081 tggtcgccgt cggccgcagc ccgaacggca acaagatcgc tgccgccaag gccggcgtcg
     1141 cggtcaccga ccgcggcttc atcccggtcg acaagcagat gcgcaccaac gtcgcgcaca
     1201 tcttcgcgat cggtgacatc gtcggtcagc cgatgctcgc ccacaaggcc gtgcacgagg
     1261 cccacgtcgc cgccgagaac gccgccggtc acaaggcctt cttcgacgcc cgccagatcc
     1321 cgtcggtcgc ctacaccgat ccggaagtgg cctgggccgg caagaccgag gaccagtgca
     1381 aggccgaagg catcgactac ggcaaggccg tgttcccgtg ggccgcatcg ggccgcgcga
     1441 tcgccaacgg tcgcgacgag ggcttcacga agctgctgtt cgacaaggcc acgcaccgca
     1501 tcgtcggcgg cgccatcgtc ggcacgcatg ccggtgacct gatcggcgag atctgcctcg
     1561 ccgtcgagat gggcgccgac ccggtcgaca tcggcaagac catccacccg cacccgacgc
     1621 tgtgcgagtc gatcggcatg gccgccgagg tcttcgaagg cgtctgcacc gacctgccgc
     1681 cgcagaagaa gaagtaagcc gcgtccgcgc gacgccggaa cggccgcctg cgggcggccg
     1741 ttttgctttg tggccaggga agtgtccaga cggtgcagca aggctctgga tcaacaaggg
     1801 aaaacccggc tttcgatctg tccgggcaac tgtccgcagc ccttgtgtcc ggtctggccg
     1861 caatccggag tgccggcgtc tgccccgtcc gtcacgtcct gccataccgt cgtgtagcgc
     1921 cgcattgatc ggttttcgtg gagctggcgc gtgcatcact aaatatccgg caggtgtttg
     1981 cagacggcag caagcgccga ctctgcggtt ccgcagctct gcatcctgcg cactgccgca
     2041 gcccggctct ggtctggcat gttgaccgtg gtgccggaag ctccggcgcc gacccgccgg
     2101 cccgagccgc gggcccgtcg agagaaccgc cgcagagagc gtgctgacca tgaagaccaa
     2161 agccgtgctg accctgaccg aggtgcgcag catcctcgat gccgccgaac agcatgcccg
     2221 cgcccagggc tgggccgtgt cgatcgccgt cgtcgatgac gggggccatg cgctcgggct
     2281 gctgcgcctc gatgacgcgt ctccggtgtc ggccgagatc gcgctcggca aggcgcgcac
     2341 cgctgcgctc gggcggcgcg acagcgaggg ctacgaggac atgatcaacc agggccgcac
     2401 ggccttcgtc agcgcgccgc tgccggtgat gctgaccggc ggcgtggtgc tgcgcatcga
     2461 cgggcaggtg atcggtgcgg tcggcgtttc cggcgtgcgt ccggagcagg acgccgagac
     2521 ggcgcgcgcc ggcatcgcag cggtggtctg agccacccga agtttcaggc aatcggcagc
     2581 ttgctgccgg cccggctcag gcggtcgtgc agcgccagca ccggcgcgac cgccggatgg
     2641 atggcggccg gacccagatg ttcgaggctg gtgcaggctg cccaggcctc ggccgcgcgt
     2701 tcgtaagccg cgatccaggc cgtgcgctgc ggctgttttt cggcggcgac cggccagaca
     2761 ccgggccgcg gcttcaggtg cgcgccgatg ccgatgcgcc agggcttggc actgacgcgg
     2821 gcgcggaagc agcgctggtt gcggcacatg cgtgcgtaca gcggatcggt gccgaccgcg
     2881 gcgaagaagg cggcgacctc ggtggcaccg ggcgcaaagg gccggtgcgt gaccagcagc
     2941 cgcagcccgg ccggcgtgcg gtagacgcgg atgccccagt ccggatgccg gtgcgcgtag
     3001 cggcgcacgc ggcgcagtgc ctgtgcctcg gcaccgccgg cgagccatcg ccacagccgg
     3061 aacaggccgt gcgcggcagg cgcgcagccg agcacggccg cgaaggccag cgcatagccg
     3121 aagccgctgc cgcaggcgtg accggcgagc gcggccgcga gcgcggcgag cacgaacacg
     3181 atgacgctca ggcgcagcgg cgcccggtgc gcatggtcga tgtccgcgaa cagcacgtcc
     3241 ggcacgttca ggcagcgcgc gccgtagctg ttgcgggtga cgacggccgt gccgtggcgc
     3301 tcgacgatct cctcgcggat cggcagaccg tcggcgccgt tgtatggctg cttcggttcg
     3361 cgccgatcca gccgttcgcc ggcgagcagg cgctgcagcg cctcgccggc gcgggtttcg
     3421 gcgtgctgct gcgcatcggc ctggctggca tcggaccagc cgtatcggcg cacggtgatc
     3481 tgccgtccct ccttgcgctg ttgccgtcgc gcttctgccc agtaggcggg gacgatcatc
     3541 ggcgcgccgg ctcagagcgc tgccagtccg tgccgcgcgc gggcacgctg acagcggtca
     3601 tcgccggcct gcagttcgcg gcacggcgac gggcgctgcg catagacggt gcaggccacg
     3661 gactgcccga tcccgccggc cagcgccacg cagcgcatcg gggctgacgc ggtgccgcgc
     3721 atgcaggcgt gccaggcgct cagggcttcg tacagcgatc cgggcaggcc gagctgcggc
     3781 gcttcgcccc agtagaacga cacgcgatag gtcgcgcagc aggcgccgca ctgcgtgcag
     3841 ggcgaggaat cggggccggg gtcgccggca ggcagcggat cggatgccgg ggccggcgtg
     3901 gccgtgctcg gttcggtcat ggcgttacgg tctgttcatg taggggaatt ctggcaccga
     3961 acctcgcggt ccgcggtcgg agcgtcacgg tccggtctgg agggcagcgg cgcgcaggcg
     4021 tcgtgcccgc agcattgccg gaacgcatgc cgatgttctc acgcatccgc acgcatcccc
     4081 gaatccgggg agcacggtga aattttcgct gtgctgcact ccgggcgggc gcgtgcggca
     4141 ccacgccgga ggcgttcgat gaagccgctt tacgatgtcg tcagtcaggg tctgatccac
     4201 agctggaagc cggtcaaggc cgccaaggcc ggccgcgcct accgctgccg ctgctcgcgc
     4261 ccggtgttct tccgcaacag cgaatgcctc gcctgccacg caccgctcgg tttcgagccg
     4321 ctgctcggcg agctgggtgc gctgcagccc ggcgatgccg agggcctgtg gcggctcgaa
     4381 aacggcgacg gcgcgcagta ccggcgctgc gcgaacttcg agcagatcgg ctgcaactgg
     4441 ctgatcccgg ccgacagcga cgcgacgctg tgcctgtcct gcgtgctgaa ccgcacgatc
     4501 ccggacctca gcgagccgga gaatctcgcg tactggcaga gcatcgagat cgccaagcgc
     4561 cagctggtcg cgcaactgct cgcactcggc ctgccggtgc gctcgaaggt cagtgacgac
     4621 ccggacaacg gcctgtgctt cgatttcctg aagtccctgc ccgacgggcc gcgcgtgctg
     4681 accggtcacg acgagggcct gatcacgctg aacgtcgagg aagccgacga cgccaggcgc
     4741 gaggcgatgc gcaagcagct cggcgagccc taccgcacgc tgctcggcca tctgcgccac
     4801 gaggtcgggc attactactg ggatgtgctc gtgcgcgact cgcactggct ggagccgtac
     4861 cgtgcgctgt tcggcgacga gtccttcgac tacgccaccg cgctgcagca gcactacgag
     4921 aacggtccgc cggccgactg gccgcagcag ttcgtcagtg cctacgcgac cagtcatccg
     4981 tgggaggact gggccgagac ctgggcgcat tacctgcacc tggtcgacac gatgaacact
     5041 gcgctcgcct tcggcctgaa cgccgaggat gtcgaggtcg acaccgagcc gttcggctcc
     5101 gatgcgctgt acgacccgca gcaccccggc gccggacagt tcctgtactt catcaacgcc
     5161 tgggtcgatc tcgtgacgat cctgaacgag ctgtcgcgca gcatgggcca gcgcgacttc
     5221 tacccgttcg tcatgagccg gccggtggtg gcgaagctgc acttcatcca tctcgtcatc
     5281 gaggacgccc gcgaccagca cctgcaggca caggacggcg gggtcgaggc cgcgacgacg
     5341 atggcggagc cggtggtgtc gtgagccatg tccggcagag ggcggttgaa cgcctcctgc
     5401 cggttcaaag cggcgtcagc gtgcgcaggc gctgcagcca ggctgcgaaa tgcgggcatt
     5461 cggcctcgat gcgttggagg ccgatgcgtc cggcggcgag cggaccatga cggaccttgc
     5521 gaaagcccgg gttgcgcaac agaccgaggc gagcggacgg cttcgtgtcc tggccgccgt
     5581 tgatctgttc aggatgatcg aagcctgaac gaattttctg cagctcaggc agatgtctgg
     5641 cccagctcgt ctccgtgccg gtcagggtgg tcagatcgga aaacaggagc gtctcgaact
     5701 cccagggctg gatatgcgga atgaagcggt caggccggca tccggtgcgt gcgatgacat
     5761 ctgcatgaaa cgccgtttcc agatacccgg cccgtctgcc gctatcggaa attttcagcg
     5821 atccagcatg cgcaggaaag gcagtgtcga gtttgtgcag gtcgaagaaa gtgctgacgt
     5881 acgtgctggc gcgttcacgc aaggtattgc gcaggaattt ccagacccgc tcatagctga
     5941 gtgctccgcc acgaccaccg cctgcaactt ccaccagccg cgctgtcagg ataatgccgc
     6001 ttgccgcgaa taccggagcc atcacgtcgc gtacgaaggt ctcttcggtc tggccttcgg
     6061 cgatgatgat gacctggatc accggcttgg tctcccgccg agcacgttct gcttccagag
     6121 ttcgccgagc gaataatcct gcagccagtc atgcaacgat tccgtatcga gccgtcgaaa
     6181 gacggattcg tcgttgatgc gatcgacgac gatgacatcc tcggcattga agtgattcag
     6241 cagttcaacc gattgcgtgg aaatgatcag ttgggtgttt tcggatactt cccgcaacat
     6301 gtcggccagc agggcaatcg catacgggtg caggccgagt tccggttcgt cgatcaggat
     6361 ggtatcgggc agcagctcgg ttggttgcag caacagcgtc gtcaggcaga tgaagcgtaa
     6421 cgtgccgtcg gacagcatgt gcgctttcag tgg
//
LOCUS       DAOQIE010000235         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6519_length_6433_cov_7.890718, whole genome shotgun sequence.
ACCESSION   DAOQIE010000235 DAOQIE010000000
VERSION     DAOQIE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6519_length_6433_cov_7.890718"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..674
                     /locus_tag="PK144_13605"
     CDS             90..674
                     /locus_tag="PK144_13605"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNF66809.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGADVQVFRNDGITPDEIERLAP
                     ARIVLSPGPCTPNEAGISMATIERFAGRIPILGVCLGHQSIGQVFGGRIVRAKQVMHG
                     KVSPVHHCGQGVFAGLPSPFTVTRYHSLVIEQATLPECLEVTAWTAGPDGSIDEIMGV
                     RHRTLAVEGVQFHPESILTEHGHALLANFLNAAA"
     gene            763..1791
                     /gene="trpD"
                     /locus_tag="PK144_13610"
     CDS             763..1791
                     /gene="trpD"
                     /locus_tag="PK144_13610"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNF66810.1"
                     /translation="MDMQSAIRRVVERQDLSGDEMRAVMRIVMTGEATPAQIGGFLVG
                     LRMKGETVDEIAAAAQVMRELATPVHAPGGHVVDIVGTGGDVSGSFNVSTAAAMVVAA
                     AGGTVAKHGNRAVSGKTGAADVLEAAGVRLDLTPAQVEQCLAEVGVAFLFAPQYHGAM
                     KHAIGPRREMAVRTIFNVLGPLTNPAGAPHELMGVFSPALVEPLAEVLLRLGCRHVLV
                     VHGEDGLDEISIGAPTLVAEGRDGRVRSYTIAPEDFGLTRAPLDAIRVGSPAESLALI
                     RRVLADEAGPARDIVVLNAGAAIHVAGLAATLAEGVARAQAVIASGAAAGKLDQLVSC
                     TRRLRDTE"
     gene            1798..2595
                     /gene="trpC"
                     /locus_tag="PK144_13615"
     CDS             1798..2595
                     /gene="trpC"
                     /locus_tag="PK144_13615"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNF66811.1"
                     /translation="MASLPDILKQIITRKHAEVAERSAALPLAQLAARAAAAPAPRGF
                     TAALREKLNAELPAVIAEAKRASPSKGLLRDPFDPAAIAASYERHGAACLSVLTDRDY
                     FQGHEDYLVAARAACTLPVIRKDFIVDPYQVVEARAIGADCILLIAAALEDEPMRALH
                     DQARALGMDVLVEVHDAEELERALALDLDMVGINNRNLRSFEVSLDTTLGLLAQIPGD
                     ILVVTESGILERADVARMREAGVHTFLVGEAFMRAPDPGAKLAELFA"
     gene            2660..3076
                     /locus_tag="PK144_13620"
     CDS             2660..3076
                     /locus_tag="PK144_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF66812.1"
                     /translation="MKASVKWLDNMTFVGESGSGHAVIMDAAPEAGGHNLGVRPMEML
                     LIGTGGCSAIDVMLILKKSRQDVTDCRVELDAERAETDPKVFTKIRMHFVVSGRGLSE
                     NHVRRAVDLSAEKYCSASIMLGQVAEVTHSYEIIAV"
     gene            3244..4026
                     /gene="speD"
                     /locus_tag="PK144_13625"
     CDS             3244..4026
                     /gene="speD"
                     /locus_tag="PK144_13625"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNF66813.1"
                     /translation="MGFNNLTKTLTFNIYDVCYTETAEEQMAYLGYIDEMYSAARLTD
                     ILSNVANIIGANILNIARQDYQPQGASVTILISEEPIAAGQLSNSTRPGPMPDTVAAH
                     LDKSHITVHTYPESHPENGISTFRADIDVSTCGRISPLKALNYLIHSFDSDIVTLDYR
                     VRGFTRDVEGRKLFIDHKINSIQNYLVQETRDRYQMIDVNVFQENIFHTKMMLKDFDL
                     NNYLFNKDKHDIAPREQVRLESLLRREMAEIFYGKNLRRGLS"
     gene            complement(4054..4701)
                     /gene="coq7"
                     /locus_tag="PK144_13630"
     CDS             complement(4054..4701)
                     /gene="coq7"
                     /locus_tag="PK144_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNF66814.1"
                     /translation="MPTRRQYSPLDHLFGTLDQAVRTVFGPPAVTERPNPAAGEQSGP
                     LSEAERREAMRLMRVNHTGEICAQALYQGQALTARLPEVRGQMERAAAEEYDHLDWCE
                     RRVQALGGHTSVLNPLFYAASFTIGALAGRAGDRWSLGFVAETEHQVVRHLDSHLERL
                     PAQDTESRAIVGQMKVDEHRHAEHALAAGGAKLPLPVRLAMRMTAKVMTGTTYWI"
     gene            4846..5166
                     /locus_tag="PK144_13635"
     CDS             4846..5166
                     /locus_tag="PK144_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNF66815.1"
                     /translation="MASHYRHHVFFCTNLREGDERPSCQRCNASAARDHAKARVKALG
                     LAGPGKVRINNAGCLDRCEEGPVIVVYPEAVWYTYVDHDDIDEIVDEHLANGRIVERL
                     RLPD"
     gene            5407..5835
                     /gene="rplM"
                     /locus_tag="PK144_13640"
     CDS             5407..5835
                     /gene="rplM"
                     /locus_tag="PK144_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNF66816.1"
                     /translation="MKTFSAKPADVKRDWFVVDATDKTLGRLSTEIARRLRGKHKAEY
                     TPHVDTGDYIVVVNAEKIRVTGNKAQDKVYYKHTGYIGNMKAFTFEKMIERAPQRVLE
                     IAVKGMLPKNPLGRAMFRKLKVFAGPTHPHAAQQPQALDI"
     gene            5868..6263
                     /gene="rpsI"
                     /locus_tag="PK144_13645"
     CDS             5868..6263
                     /gene="rpsI"
                     /locus_tag="PK144_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNF66817.1"
                     /translation="MADNQFYGTGRRKTSTARVFLRTNGKGDIVVNGRPLDIYFGRET
                     SRMVVRQALEAVDALGRFDFYITVAGGGMTGQAGAIRHGITRALMAYDESLRPTLRKA
                     GFVTRDARAVERKKIGLRKARRAVQFSKR"
     gene            6327..6400
                     /locus_tag="PK144_13650"
     tRNA            6327..6400
                     /locus_tag="PK144_13650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6359..6361,aa:Gln,seq:ctg)
BASE COUNT         1088 a         2307 c         2085 g          953 t
ORIGIN      
        1 gcgagcgaat gggaggaaac cctgaacaag ggccgcgcga tcttccgcgc cgccgcactc
       61 gccggccgcg gcttcggagg ctgacgatca tgctgctgat gatcgataac tacgactcgt
      121 tcacgtacaa cctcgtgcag tacttcggcg aactcggggc cgacgtgcag gtgttccgca
      181 acgacggcat cacgcccgac gagatcgagc ggctggcgcc ggcgcgcatc gtgctgtcac
      241 cggggccgtg cacgccgaac gaggccggca tctcgatggc gacgatcgaa cgcttcgccg
      301 ggcgcatccc gatcctcggc gtctgcctcg gccaccagag catcggccag gtgttcggcg
      361 ggcgcatcgt gcgcgcgaag caggtcatgc acggcaaggt ctcgccggtg catcactgcg
      421 gccagggcgt gttcgccggt ctgccgagcc cgttcacggt cacgcgctac cactcgctgg
      481 tcatcgagca ggcgacgctg cccgagtgcc tggaggtcac cgcgtggacg gccggccctg
      541 acgggtcgat cgacgagatc atgggcgtgc gccaccggac gctggcggtc gagggcgtgc
      601 agttccaccc ggaatcgatc ctgaccgaac acggccatgc gctgctcgcg aacttcctga
      661 acgcagcggc ctgaggccgc ctgcgcggcg gctccggcgt cgggcggcgc cggggcaggc
      721 cgcgcgccag cttcccgttt cctccaaacc cgagggttcg acatggacat gcagtccgcc
      781 atccgccgcg tcgtcgagcg ccaggatctc agtggtgacg agatgcgtgc ggtcatgcgc
      841 atcgtcatga ccggcgaggc cacgccggcg cagatcggcg gctttctggt cggcctgcgc
      901 atgaagggcg agaccgtcga cgagatcgca gccgccgcgc aggtgatgcg cgagctggcg
      961 accccggtgc atgcgccggg cgggcacgtc gtcgacatcg tcggcaccgg cggcgatgtc
     1021 tccggcagct tcaacgtctc gacggccgcg gcgatggtcg ttgccgctgc cggcggcacg
     1081 gtcgccaagc acggcaaccg cgccgtcagc ggcaagaccg gcgcggccga cgtgctcgaa
     1141 gccgccggcg tgcggctgga tctgacgccg gcgcaggtcg agcagtgcct cgccgaggtc
     1201 ggcgtcgcct tcctgttcgc gccgcagtac cacggcgcca tgaagcacgc gatcggtccg
     1261 cgccgcgaga tggcggtgcg cacgatcttc aacgtgctcg ggccgctgac caaccccgcc
     1321 ggcgcgccgc acgagctgat gggcgtgttc agcccggcgc tggtcgagcc gctcgccgag
     1381 gtgctgctgc gcctcggctg ccggcacgtg ctggtcgtgc acggtgagga cgggctcgat
     1441 gaaatctcga tcggcgcccc gacgctagtc gccgaagggc gcgacggccg ggtgcgcagc
     1501 tacacgatcg cgcccgagga cttcggcctg acgcgcgcac cgctcgatgc catccgcgtc
     1561 ggctcgccgg ccgaatcgct ggcgctgatc cggcgcgtgc tcgccgatga ggccggcccg
     1621 gcgcgcgaca tcgtcgtgct gaacgccggc gccgcgatcc acgtcgccgg ccttgccgcg
     1681 acgctcgccg aaggcgtcgc gcgcgcacag gcggtgatcg ccagcggggc cgccgccgga
     1741 aaactcgacc agctcgtatc ctgcacccga cgcctgcgcg acacggagta gtccatgatg
     1801 gccagccttc ccgacatcct gaagcagatc atcacccgca agcacgccga ggtcgccgag
     1861 cgcagcgcag cgctgccgct cgcgcagctc gccgcccgtg ccgcggccgc cccggcaccg
     1921 cgcggcttca ccgcggcgct gcgcgagaag ctcaacgccg agctgccggc ggtgatcgcc
     1981 gaggccaagc gcgcctcgcc gagcaagggc ctgctgcgcg atcccttcga tccggccgcg
     2041 atcgccgcga gctacgaacg ccacggtgcg gcctgcctgt cggtgctgac cgaccgcgac
     2101 tacttccagg gccacgagga ctacctcgtc gccgcgcgcg ccgcctgcac gctgccggtg
     2161 atccgcaagg acttcatcgt cgatccgtat caggtggtcg aggcgcgcgc gatcggcgcc
     2221 gactgcatcc tgctgatcgc cgccgcgctc gaggacgagc cgatgcgcgc gctgcacgat
     2281 caggcgcgcg cactcggcat ggacgtgctg gtcgaggtcc acgatgccga ggagctcgaa
     2341 cgcgcgctcg cgctcgatct ggacatggtc ggcatcaaca accgcaacct gcgcagcttc
     2401 gaggtctcgc tcgacaccac gctcggcctg ctcgcgcaga tccccggcga catcctcgtc
     2461 gtcaccgaga gcggcatcct cgaacgcgcc gacgtggcac gcatgcgcga ggccggcgtg
     2521 cataccttcc tggtcggcga ggccttcatg cgcgcgccgg acccgggcgc gaagctcgcc
     2581 gagctgttcg cgtgagggcg gccgcgccgt cgccgccttg ccggtttccc cgttcccgtc
     2641 ccctacggaa gtccctgcga tgaaagcaag cgtcaagtgg ctcgacaaca tgaccttcgt
     2701 cggcgagtcc ggcagcggcc acgccgtgat catggatgcc gcgcccgagg ccggcggcca
     2761 caacctcggc gtgcggccga tggagatgct gctgatcggc accggcggct gctcggcgat
     2821 cgacgtgatg ctgatcctga agaagtcgcg tcaggacgtg accgactgcc gcgtcgagct
     2881 cgacgccgag cgcgccgaaa ccgacccgaa ggtgttcacg aagatccgca tgcacttcgt
     2941 cgtcagcggc cgcggcctgt ccgagaacca cgtgcgccgc gccgtcgacc tgtcggccga
     3001 aaagtactgc tcagcctcga tcatgctcgg tcaggtcgcc gaagtgacgc acagctacga
     3061 gatcatcgcc gtctgaagcc ccggatcgcc ggcgttgtgc cggtgccaaa atgcctttgc
     3121 cgccgatgac ttgtcatcgg cattcggtat actcgcgcct tttttaagcc agcccggccc
     3181 gacatggccg gcaacggagg aaaagccgcg gtgaaaccga agccactgcc ccgactgaag
     3241 ctgatggggt tcaacaacct caccaagacg ctgacgttca acatctacga cgtctgctac
     3301 accgagacgg ccgaggagca gatggcgtat ctcggctaca tcgacgagat gtacagcgcc
     3361 gcacgcctga ccgacatcct cagcaacgtc gccaacatca tcggcgccaa catcctgaac
     3421 atcgcgcgtc aggactatca gccgcagggc gcgagcgtca cgatcctgat ctccgaggag
     3481 ccgatcgccg ccggccagct gtcgaactcg acgcgtcccg gcccgatgcc ggacacggtc
     3541 gccgcgcacc tcgacaagag ccacatcacg gtgcacacgt atccggagag ccatccggag
     3601 aacggcatca gcaccttccg cgccgacatc gatgtctcga cctgcggacg catctcgccg
     3661 ctgaaggcgc tgaactacct gatccacagc ttcgactccg acatcgtcac gctcgattac
     3721 cgcgtgcgcg gcttcacgcg cgatgtcgag ggcaggaagc tgttcatcga ccacaagatc
     3781 aactcgatcc agaactacct ggtgcaggaa acccgcgacc gctaccagat gatcgacgtg
     3841 aacgtctttc aggagaacat cttccacacg aagatgatgc tgaaggactt cgatctgaat
     3901 aactacctgt tcaacaagga caagcacgac atcgcgccgc gcgagcaggt acggctcgaa
     3961 tcgctgctgc gccgcgagat ggccgagatc ttctacggca agaacctgcg ccgcggcctg
     4021 tcctgaggcg cagggccggt ttcagacccg ctttcagatc cagtacgtcg tgccggtcat
     4081 gaccttcgcg gtcatgcgca tcgccaggcg caccggcagc ggcagcttcg cgccgccggc
     4141 ggcgagcgcg tgctcggcgt ggcgatgctc gtcgaccttc atctgcccga cgatcgcgcg
     4201 gctctcggtg tcctgcgcgg gcaggcgttc gaggtggctg tcgaggtggc gcacgacctg
     4261 atgctcggtc tcggcgacga agccgaggct ccagcggtca ccggcgcgtc cggccagcgc
     4321 gccgatcgtg aacgacgcgg cgtagaacag cggattcagc acgctggtat gcccgccgag
     4381 cgcctgcaca cgccgctcgc accagtcgag atggtcgtac tcctcggccg ccgcgcgctc
     4441 catctggccg cgcacctcgg gcagccgcgc ggtcagcgcc tgcccctgat acagcgcctg
     4501 cgcgcagatc tcgccggtat ggttgacgcg catcagccgc atcgcctcgc gccgctcggc
     4561 ctcgctgagc gggccggact gctcgccggc cgccgggttc gggcgttccg tgaccgccgg
     4621 cggtccgaac accgtgcgca cggcctgatc gagcgtgccg aacaggtggt cgagcggtga
     4681 gtactggcga cgggtgggca tggcggcggg ctcctgttgc agcgcaaggt ccggccatcc
     4741 taagcccggt gccgcaccgg cggaaacccg gcacggcggt gctggccgcc tgccggcacc
     4801 ggcgcgatac tcgggcgctc cccgccgctc accggagccc cccccatggc cagccactac
     4861 cgccaccacg tgttcttctg caccaacctg cgcgaaggcg acgagcgtcc gagctgccag
     4921 cgctgcaacg ccagcgccgc gcgcgatcac gccaaggcgc gcgtcaaggc gctcggcctc
     4981 gccggccccg gcaaggtgcg catcaacaac gccggctgcc tcgaccgctg cgaggaaggg
     5041 ccggtcatcg tcgtctaccc ggaggcggtc tggtacacct acgtcgatca tgacgacatc
     5101 gacgagatcg tcgacgagca cctcgccaac ggccgcatcg tcgagcgcct gcgcctgccg
     5161 gactgaaatc acagcaaaat cagccctccg cgcacgcagc gcggccggac aaaaggcttg
     5221 tcacccgcgc cgggcctgat tactatgcgc gccctcgacc ggagcgcagg ccctcgtgcc
     5281 gtgcccggac accgaaccga tccagattcc atcagatcga aacagaattc ccgagtcccg
     5341 caccagcagc gcggtcgacg ccggcagcgg cagcgatccg gatcggggct catttccgga
     5401 gtgaacatga agaccttcag cgccaagccg gcggacgtca agcgtgactg gttcgtcgtc
     5461 gatgccaccg acaagaccct cggccgcctg tcgaccgaga tcgcccgccg cctgcgtggc
     5521 aagcacaagg ccgagtacac gccgcacgtc gacaccggtg actacatcgt cgtcgtcaac
     5581 gccgagaaga tccgcgtcac cggcaacaag gcgcaggaca aggtctacta caagcacacc
     5641 ggctacatcg gcaacatgaa ggccttcacc ttcgagaaga tgatcgagcg cgccccgcag
     5701 cgcgtgctcg agatcgccgt gaagggcatg ctgccgaaga acccgctggg gcgtgcgatg
     5761 ttccgcaagc tcaaggtctt cgccggcccg acccacccgc acgcggccca gcagccgcag
     5821 gccctcgaca tctgagcccc gcggcttccg agacaaggtt tcaggacatg gctgacaatc
     5881 agttctacgg caccggccgt cgcaagacct cgaccgcccg ggtgttcctg cgcaccaacg
     5941 gcaagggcga catcgtcgtc aacggccgtc cgctcgacat ctacttcggt cgcgagacct
     6001 cgcgcatggt cgtgcgccag gcgctcgaag ccgtcgacgc gctgggtcgc ttcgacttct
     6061 acatcaccgt cgccggtggc ggcatgaccg gccaggccgg cgcgatccgc cacggcatca
     6121 cccgtgcgct gatggcctac gacgagtccc tgcgtccgac gctgcgcaag gccggcttcg
     6181 tgacccgcga tgcccgtgcc gtcgagcgca agaagatcgg cctgcgcaag gcccgtcgcg
     6241 cggtgcagtt ctcgaagcgc tgatgcaata ttcgtaccgg tcttgcagtt tcgcactgca
     6301 atcggtacga tacgcggcac gtttcttggg ggatcgtcta gcggcaggac tacggactct
     6361 gactccgtca acctaggttc gaatcctagt cccccagcca aacacacaga gcccgcatcg
     6421 aaagatgcgg gct
//
LOCUS       DAOQIE010000236         6393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6581_length_6393_cov_7.152887, whole genome shotgun sequence.
ACCESSION   DAOQIE010000236 DAOQIE010000000
VERSION     DAOQIE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6581_length_6393_cov_7.152887"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3434)
                     /gene="dnaE"
                     /locus_tag="PK144_13655"
     CDS             complement(<1..3434)
                     /gene="dnaE"
                     /locus_tag="PK144_13655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNF66818.1"
                     /translation="MSATYVHLHVHTEFSLLDSLMPVKPLVKAVGKAGMPAVAVTDLG
                     NLFALIKFYKAAQGSGVKPVVGCEVHIGRGGSEPCGRLVLLCRNDEGYRNLTVLVSRG
                     YVDGQQRGVPVVHIDWLREHAGGLIALSAARDGEIGKAIANGNLAQARALCDEYAALF
                     PDAFYLELQRTGRPGETEYLARAVELAAGRGLPVVATNDVRFLARADFEAHEARVCIA
                     QGWTLADPKRPRDYSEQQYLRTAAEMAELFADLPEALENSVEIARRCNLTLTLGKPRL
                     PNFPVPAGMSVDDYFAEASRRGLQERFAKMFDATAEDYAERTRPYRERLETEIGVIVQ
                     MGFPGYFLIVADFIQWAKSQQIPVGPGRGSGAGSLVAYALKITDLDPLPYDLLFERFL
                     NPERVSMPDFDVDFCMEGRDRVIDYVAQTYGRDSVSQIATHGTMAAKAVIRDCGRVLG
                     HPYGFVDKVAKLVPLELGITLADALEKDEDFRKAYDGDEEIRALVDLGQKLEGLARSV
                     GKHAGGVVISPTVLTDFSPLYCEEGSRQLVTQFDKDDVEAAGLVKFDFLGLRTLTIID
                     WALATLNRQRAGKGEPPIDVMALPLDDAPAYELLKRCETTAVFQLESRGMKDLIRRLQ
                     PDCFEDIVALVALFRPGPLESGMVDDFINRKHGRAKVEYPFPECEPVLKPTYGVIVYQ
                     EQVMQIPQVLAGYTLGGADLLRRAMGKKKPEEMAKQRDIFVSGATANGHDRDKAGALF
                     DLMEKFAGYGFNKSHSAAYALVSYHTAWLKAHYPAAFMAAVLSSDMDKTDKVVVFIEE
                     CRRMRLAVVPPSVNVSEFKFTVDAAGAVVYGLGAVKGVGENAIEAIIRERTASGAFRD
                     LFDFCRRVDLKKLNRRVLEALIRAGAFDVFGDTRATLMACLPEAIRLAEQHARNDEVG
                     QCDLFGLMADAGPAVDVARIDVPKLPEWDPDEKLAGEKETLGLYLTGHPIDRYLPELR
                     QFTKGSLTDALEEAETGFQAPGARRYGREAEKPALLAGLVVDIRIKQGARGRMAFVTL
                     DDRGGRLEVRVFGDTCEQYRALLAVDRVLVIDAALSWDDYSGGVRATAREIMDFDTAR
                     EKHARRIRVKLADPDVRLAARLAEALEAHRGGKCRVVLEVSTPEAGGELVCGEGWQV"
     gene            3530..4312
                     /locus_tag="PK144_13660"
     CDS             3530..4312
                     /locus_tag="PK144_13660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008117.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HNF66819.1"
                     /translation="MRRPPPPRPAAPRGLAADEAKIHGANACAVTLRERPDAVVRAWF
                     AESTARHWAEAMQGLAARRRAYHVVPDDELGLVAGSEHHGGVCLIVRKKRPVSIERWL
                     TKMEKRERVCGLALEGVGNPHNLGAILRTGAHFAIDGLLVADTDALHSGACARVAEGG
                     LEYVETVHCAHTGDAIRLARRAGFGILTTSSHGGEPLYAAELPPKCLIVFGEESQGLT
                     KQTLKAGDVQVMIPGSGAVESLNVSVAAAVIVAEWWRRFRQP"
     gene            complement(4322..4915)
                     /gene="rnhB"
                     /locus_tag="PK144_13665"
     CDS             complement(4322..4915)
                     /gene="rnhB"
                     /locus_tag="PK144_13665"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNF66820.1"
                     /translation="MFHAPDAPTLIAGCDEAGRGPLAGAVVAAAVILDPARPIAGLAD
                     SKTLSAARREALAGLIRERALAWAIGRAEADEIDRINILWASMRAMERAVAALGVVPQ
                     LVQVDGNRVPPGLAMPGEAIVKGDAKVPAIGAASILAKVARDAELLVLDRDFPQYGFA
                     QHKGYPTAAHLAALVEHGPCPAHRRSFGPVRRALGLA"
     gene            complement(4946..5260)
                     /locus_tag="PK144_13670"
     CDS             complement(4946..5260)
                     /locus_tag="PK144_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF66821.1"
                     /translation="MDEHEFEQLLASVREMGRHRRGETVAGLRVSEWSEPDVRSIRAA
                     TQLSQTKFAALIGVRLRTLQAWENRRSRPIGPARALLRIVERDPQALIALRAELPARH
                     PV"
     gene            complement(5253..5468)
                     /locus_tag="PK144_13675"
     CDS             complement(5253..5468)
                     /locus_tag="PK144_13675"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF66822.1"
                     /translation="MLIPGAEGLRKLRVELPGRGRRGGARVICYWWTAQDRLYLLYAY
                     AKNVQSDLTADQVRRLAAVMRAETGNG"
     gene            complement(5639..>6393)
                     /gene="lpxB"
                     /locus_tag="PK144_13680"
     CDS             complement(5639..>6393)
                     /gene="lpxB"
                     /locus_tag="PK144_13680"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF66823.1"
                     /translation="RGIAQSVDLMLTLFPFEATFYAQHHVPVECVGHPLADEIPAGLT
                     VADGRAALGLGAGRSLALLPGSRAGEVGRHIDVFLAAFARLHERHPQLNALLPCATAA
                     LRAQIAARVTAAGLDAQVRLFDGHAREVLAASELALVASGTATLETMLVGRPLVMAYR
                     VAPLTYAIAKRLMKIDRFSLPNLLAGHDLVPEFIQDAATPGALAAALDAWLREPARVQ
                     AACTEFAGLHERLRCNAGERAAAALAGLLRQA"
BASE COUNT          953 a         2269 c         2241 g          930 t
ORIGIN      
        1 acctgccagc cttcgccgca gaccagttcg ccgccggcct ccggggtgct gacttcgagc
       61 accacgcggc acttgccgcc gcggtgggct tcgagcgcct cggccaggcg cgcggcgagg
      121 cgcacgtccg ggtcggcgag cttgacgcgg atgcgccggg cgtgcttctc gcgggcggtg
      181 tcgaaatcca tgatctcgcg cgcggtcgcg cgcacgccgc cggagtagtc gtcccaggac
      241 agcgccgcgt cgatgaccag cacgcggtcg acggcgagca gcgcgcggta ctgctcgcag
      301 gtgtcgccga acacgcgcac ctccagccgg ccgccgcgat cgtcgagcgt cacaaaggcc
      361 atgcgcccgc gcgcgccctg cttgatgcgg atgtcgacca ccagcccggc gagcagcgcc
      421 ggtttctcgg cctcgcggcc gtagcgccgg gcgccgggcg cctggaagcc ggtttcggcc
      481 tcctccagcg cgtcggtcag gctgcccttg gtgaactggc gcagctccgg caggtagcgg
      541 tcgatcgggt ggccggtcag gtacaggccg agcgtttcct tctcgccggc gagcttttcg
      601 tccgggtccc actccggcag cttcggcacg tcgatgcgcg cgacatcgac ggccgggccg
      661 gcgtcggcca tcagcccgaa caggtcgcac tggccgacct cgtcgttgcg cgcgtgctgc
      721 tcggcgaggc ggatcgcctc gggcagacag gccatcagcg tggcgcgcgt gtcgccgaac
      781 acgtcgaagg cgccggcacg gatcagggct tcgagcacgc ggcggttgag cttcttcaga
      841 tccacgcgcc ggcagaagtc gaacaggtcg cggaaggcgc cgctggccgt gcgctcgcgg
      901 atgatcgcct cgatggcgtt ctcgccgacg cccttgaccg cgccgaggcc gtagacgacg
      961 gcaccggcgg catcgaccgt gaacttgaac tccgagacgt tcaccgacgg cggcacgacc
     1021 gcgaggcgca tgcgccggca ttcctcgatg aacacgacga ccttgtcggt cttgtccatg
     1081 tcggacgaca gcaccgcggc catgaacgcc gccgggtaat gcgccttcag ccaggcggtg
     1141 tggtagctga ccagcgcgta ggcggccgaa tgcgacttgt tgaagccgta gccggcgaac
     1201 ttctccatca ggtcgaacag cgcgccggcc ttgtcgcggt cgtggccgtt ggcggtggcg
     1261 ccgctgacga agatgtcgcg ctgcttggcc atctcctcgg gcttcttctt gcccatggcc
     1321 cggcgcagca ggtcggcacc gccgagcgtg tagccggcga gcacctgcgg gatctgcatg
     1381 acctgttcct gatagacgat gacgccatac gtcggcttca ggaccggctc gcattccggg
     1441 aacgggtatt cgaccttggc gcggccgtgc ttgcggttga tgaagtcatc gaccatgccc
     1501 gattcgagcg gcccgggccg gaacagcgcc accagcgcga cgatgtcctc gaagcagtcg
     1561 ggctgcaggc ggcggatcaa atccttcatg ccgcgcgatt cgagctggaa caccgcggtg
     1621 gtctcgcagc gcttgagcag ctcgtaggcg ggcgcgtcat cgagcggcag cgccatcacg
     1681 tcgatcggcg gctcgccctt tccggcacgc tggcggttca gcgtcgccag cgcccagtcg
     1741 atgatggtca gcgtgcgcag gccgaggaag tcgaacttga cgaggccggc ggcctcgaca
     1801 tcgtccttgt cgaactgcgt gaccagctgg cggctgcctt cttcgcagta cagcggcgag
     1861 aaatcggtca gcacggtcgg cgagatcacc acgccgccgg cgtgcttgcc gaccgagcgc
     1921 gccagccctt cgagcttctg gccgagatcg accagcgcgc ggatctcctc gtcgccgtcg
     1981 taggccttgc ggaagtcctc gtccttctcc agcgcatcgg cgagcgtgat gccgagttcg
     2041 agcggcacga gcttggcgac cttgtcgacg aagccgtacg gatgaccgag cacgcggccg
     2101 cagtcgcgga tcaccgcctt ggccgccatc gtgccgtgcg tggcgatctg cgaaacgctg
     2161 tcgcggccat aggtctgcgc gacgtagtcg atgacgcgat cgcggccttc catgcagaag
     2221 tcgacgtcga agtccggcat cgacacgcgc tcggggttca ggaagcgctc gaacaaaagg
     2281 tcatacggca gcgggtcgag gtcggtgatc ttcagcgcat acgcgaccag cgaaccggcg
     2341 cccgagccgc gccccggtcc gaccgggatc tgctggctct tcgcccactg gatgaagtcg
     2401 gcgacgatca ggaagtagcc gggaaagccc atctgcacga tgacgccgat ctcggtttcc
     2461 aggcgctcgc ggtacggccg ggtgcgctcg gcgtaatcct cggccgtggc atcgaacatc
     2521 ttcgcgaagc gctcctgcag cccgcggcgc gaggcttcgg cgaagtagtc atcgacgctc
     2581 atgccggccg gcaccgggaa gttcggcagc cgtggcttgc cgagcgtcag cgtcaggttg
     2641 cagcgcctgg cgatctcgac gctgttctca agcgcctcgg gcagatcagc gaacagttcc
     2701 gccatctcgg cggcggtgcg caggtactgc tgctcggagt agtcgcgcgg gcgcttcgga
     2761 tcggcgagcg tccagccctg cgcgatgcag acgcgcgcct cgtgcgcctc gaagtcggcg
     2821 cgcgcgagga agcgcacgtc gttggtcgcg accaccggca gcccgcgtcc ggcggcgagt
     2881 tcgaccgcgc gcgcgaggta ttcggtctcg cccgggcggc cggtgcgctg cagttcgagg
     2941 tagaaggcat ccgggaacag cgccgcgtat tcatcgcaga gcgcgcgcgc ctgcgcgagg
     3001 ttgccattgg caatcgcctt gccgatctcg ccgtcgcgcg ccgccgacag cgcgatcagc
     3061 ccgccggcgt gctcgcgcag ccagtcgatg tgcacgaccg gcacgccgcg ctgctggccg
     3121 tcgacgtagc cgcgcgagac cagcacggtc aggttgcggt agccctcgtc gttgcgacag
     3181 agcagcacca gccggccgca aggctcgctg ccgccgcgac cgatgtggac ctcgcagccg
     3241 accaccggct tgacgcccga gccctgcgcg gccttgtaga acttgatcag cgcgaacagg
     3301 ttgccgagat cggtcaccgc caccgccggc atgccggcct tgccgaccgc cttcaccagc
     3361 ggcttgaccg gcatcaggct gtcgagcagc gagaactcgg tgtggacgtg caggtggacg
     3421 taggtcgcgc tcatcacgga tcgccgggca gcactggggg cgcgcagggt aacgcagccg
     3481 cttcggcccg ggataggatg cgcgcccgcc cccgaggaac cgcatcgcca tgcgccgccc
     3541 gcccccgccc cgccctgccg ccccgcgcgg cctcgccgcc gacgaggcga agatccacgg
     3601 cgccaatgcc tgtgcggtga cgctgcgcga gcggcccgat gccgtggtgc gcgcgtggtt
     3661 cgccgagagc acggcgcgcc actgggccga ggcgatgcag ggcctcgcgg cgcgccggcg
     3721 cgcgtatcac gtcgtgccgg acgacgaact cggcctcgtc gccggcagcg agcaccacgg
     3781 cggcgtctgc ctgatcgtgc gcaagaagcg gccggtgtcg atcgagcgct ggctcacgaa
     3841 gatggaaaaa cgcgagcggg tctgcgggct cgcgctcgaa ggcgtcggca acccgcacaa
     3901 cctcggcgcg atcctgcgca ccggcgcgca cttcgcgatc gacggcctgc tggtcgccga
     3961 taccgacgcg ctgcattccg gggcctgcgc gcgcgtcgcc gagggcgggc tggaatacgt
     4021 cgagaccgtg cactgcgcgc ataccggcga cgccatccgc ctcgcccgcc gcgccggctt
     4081 cggcatcctg accacgtcga gtcacggcgg cgagccgctg tatgcggccg agctgccgcc
     4141 gaagtgcctg atcgtgttcg gcgaggagtc gcagggactg acgaagcaga cgctgaaggc
     4201 cggcgacgtg caggtgatga tccccggcag cggcgcggtc gagagcctga acgtgtcggt
     4261 ggcggcggcg gtgatcgtcg ccgagtggtg gcggcgcttc cgccagccct gagcaccgga
     4321 ctcaggccag gccgagcgcc ctgcgcaccg gcccgaacga gcgccggtgc gccggacacg
     4381 ggccgtgctc gaccagggcc gcgaggtgcg cggcggtcgg atagcccttg tgctgcgcga
     4441 agccgtactg cggaaagtcg cgatcaagca cgagcagttc ggcatcgcgc gcgaccttgg
     4501 cgaggatcga ggccgctccg atcgccggca ccttcgcatc acccttgacg atcgcctcgc
     4561 ccggcatcgc gagccccggc ggcacgcggt tgccgtcgac ctgcacgagc tgcggcacga
     4621 cgccgagcgc ggccaccgcg cgctccatcg cccgcatcga ggcccacagg atgttgatgc
     4681 ggtcgatctc gtcggcctcg gcccggccga tcgcccaggc cagcgcccgc tcgcggatga
     4741 gtccggccag cgcctcgcgc cgtgcggcac tgagcgtctt ggaatcggcg agccccgcga
     4801 tcggccgggc cggatcgagg atcaccgccg cggcgaccac agccccggcg agcggcccgc
     4861 gaccggcctc gtcgcagccg gcgatcaggg tcggtgcatc cggagcgtga aacaggtcga
     4921 ggctgagatt cttcacgatc tggactcaaa ccggatgccg ggcaggaagc tcggcgcgca
     4981 aggcgatcag cgcctgcgga tcgcgctcga cgatgcgcag cagcgcccgg gccgggccga
     5041 tcggccgcga gcgccggttt tcccaggctt gcagcgtgcg caggcgcacg ccgatcagcg
     5101 cggcaaactt cgtctgtgac agctgcgtgg ctgcgcggat cgagcggacg tcgggttcgg
     5161 accactcgct gacccgcagg ccggccacgg tctcgccgcg ccgatgccgc cccatctcgc
     5221 gcacgcttgc cagcaactgc tcgaattcgt gctcatccat tcccggtctc cgcccgcatc
     5281 acggctgcca gtcgccgcac ctgatccgcc gtcaggtccg actggacgtt cttggcatac
     5341 gcatacaaca ggtacagccg atcctgtgct gtccaccagt agcagatcac tcgcgccccg
     5401 ccccgtcgtc cgcgtcccgg cagctcgaca cgcagcttgc gcagcccttc tgcaccgggg
     5461 atcagcacgc ctgccgccgg atcggcgagc agccgctctc ctgcaggtgg cgcaatccct
     5521 catcatcaaa caggcttgcg gcgacacgca cgaaatgggc ggtttccacg aagatcatcc
     5581 ttgcctccgt actgccgggt ctgcaaccgg gagggagtgg agtgcggcac gaacctgctc
     5641 aagcctgccg cagcagcccg gcaagcgccg ccgccgcccg ttcgccggca ttgcagcgca
     5701 ggcgctcgtg caggccggca aactcggtgc atgccgcctg cacgcgcgcc ggctcgcgca
     5761 gccaggcatc gagcgccgcg gccagcgccc cgggggtcgc ggcgtcctgg atgaactcgg
     5821 ggaccagatc gtggccggcg agcaggttcg gcagcgagaa gcggtcgatc ttcatcagcc
     5881 gcttcgcgat cgcgtaggtc agcggcgcga cgcggtaggc catgaccagc ggccggccga
     5941 ccagcatcgt ctccagcgtc gcggtgccgg aggcgaccag cgccagctcg ctggcggcga
     6001 gcacctcgcg cgcgtggccg tcgaacagcc gcacctgtgc gtcgagcccg gctgccgtca
     6061 cgcgcgcggc gatctgtgcg cgcagcgcgg cggtcgcgca cggcagcagc gcattgagct
     6121 gcggatgccg ttcgtgcagc cgcgcgaagg ccgcgaggaa cacgtcgatg tgccggccga
     6181 cctcgccggc gcggctgccg ggcagcaagg cgaggcttcg gccggccccg agcccgagcg
     6241 cggcgcggcc gtcggcgacc gtgaggcccg cagggatttc gtccgccagc ggatgaccga
     6301 cgcattcgac cggcacgtga tgctgcgcgt agaacgtcgc ctcaaacgga aacagcgtca
     6361 gcatcaggtc gacgctctgc gcgatgccgc gca
//
LOCUS       DAOQIE010000237         6319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6692_length_6319_cov_7.014049, whole genome shotgun sequence.
ACCESSION   DAOQIE010000237 DAOQIE010000000
VERSION     DAOQIE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6692_length_6319_cov_7.014049"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PK144_13685"
     CDS             complement(<1..936)
                     /locus_tag="PK144_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF66824.1"
                     /translation="MGGCADAGEAGAARLPRIAIACQGGGSHTAFTAGVLQQLLAAAG
                     ADGRFTIAGFSGSSGGAICALLAWYGLLLGSPAEGVRLLEDFWHDNAAETLPDQLLNE
                     FVLGSARLRDHLLLPELSPYDVPNWGQDQLRSLLEWHVEFERIPQLTGPGRPSLFVGA
                     VDVLAGCARSFRDAEITVATLLASTATPTLFRAVRVGAGHYWDGLFTQNPPIRELAAL
                     APDELWVVQINPCSTPELPRTLDAIRDRHGELAGHLALEQALGTIELVNRLLASGELA
                     PGRFRPIRVHRLQLARTLDHAAKRDRNPAFLRGLMR"
     gene            complement(941..1609)
                     /locus_tag="PK144_13690"
     CDS             complement(941..1609)
                     /locus_tag="PK144_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNF66825.1"
                     /translation="MEHAYQHRDRDGQPIDLPVGKIVCIGRNYLDHVRELGNAVPEAP
                     LLFMKPSTAIVGLDAPLRLPEARGSCHHELELAVLIGRRLTGTSPAAAREAIAGYGLA
                     LDLTLREVQTELKNKGQPWELAKAFDGACPLSGFVRPEELPEPQAAELELLVNGERRQ
                     HGSTAQMMHPILDLIAHISRHFTLLPGDVVLTGTPAGVGPLASGDRLELRLAGRWSWS
                     ARVA"
     gene            1868..2947
                     /locus_tag="PK144_13695"
     CDS             1868..2947
                     /locus_tag="PK144_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF66826.1"
                     /translation="MPMLDLPDCRLHYRETGSGAPLLLLHGNFASQQWWAPQFADPPA
                     GRRLIAPDLRGLGDSGRPGHGYDVPCAAADVLALLDALDIERCDVVGHSLGGVVALQL
                     AILQPQRIRSLLLLTPAPADGIRLDHLGPLAWLGAERIARLIPTLTQHHATMDHLLPH
                     ALPGLREDAAQIAKLCEIALACDPAAIAGYTGALAGLRLLPALATLDQPVLVLAGALD
                     PLVSPQSLEPTVAQLRHGALLIWSDCGHAPQLEQVQPFRTLLADWLADASAVLADIGL
                     HGVVNPPPIVIEPPPPEQLLETTVPLTLPALQPAANELLQSAFEAAQQATQAGTAASV
                     AAVETAVQAQAGLLQRLRSWLHLGD"
     gene            complement(2968..5373)
                     /gene="ligA"
                     /locus_tag="PK144_13700"
     CDS             complement(2968..5373)
                     /gene="ligA"
                     /locus_tag="PK144_13700"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNF66827.1"
                     /translation="MSHLDPHARLLQLRDEIRRHEYLYYVLDQPEVPDAVFDALVIEL
                     RGIEDGHPQWITPDSPSQRVGGRPAAGFAPVRHALPMLSLDNAFTEADVRDFDRRARE
                     RLAAAGIEVDAIAYSAEPKLDGLAVSLRYEHGLLRQAATRGDGTTGEDITANVRTIRA
                     IPLRLAGGGWPEVLEVRGEVFMLRRDFERLNERARERGDKTFANPRNAAAGSLRQLDP
                     AVTAGRRLSFYAYGLGEVSDERALPGEHSAIIERLGDWELPVCPQRAVVFGPDGCLGY
                     YAGIGVQRAALAYDIDGVVYKVDRLDWQRRLGFVARAPRWASAHKYPAQEQLTTVEAI
                     DVQVGRTGAITPVARLEPVFVGGVTVTNATLHNFDEVARKDVRIGDTVIVRRAGDVIP
                     EVAGVLLDRRPMRSLLDPEHEPWQPPQHCPVCGSHIERPEGEAIARCSGGLTCAAQRK
                     EAIRHFAARRALDIDGLGERYIENLVDLGYVHGVADLYRLTLDDFLEMKRRADERDGI
                     TPETVAQGRIATKWAENLIGALARSRHTTLARLLFALGIRHVGESTAKTLADWLGTLE
                     RVRHAPAPVLRALPDIGPEVAEAIDEFFLQPGNQQALDALLAGGVQPADEHPPAAALA
                     ERLNPVALLAALGAPKLSEARARQLAPALGAQGLAALPDLLADEARLAGTGLPAGHVQ
                     ALRDWLAVPESRARVQRTAGFAAGLLALLPAAPLIVRPLDGLTVVLTGTLSAMTRDEA
                     RARLEALGAKVAGSVSKKTGYVVAGAEAGSKLAKAQELGVEVLDEAAFLQRLAQWESA
                     GGG"
     gene            complement(5749..>6319)
                     /locus_tag="PK144_13705"
     CDS             complement(5749..>6319)
                     /locus_tag="PK144_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNF66828.1"
                     /translation="GMTWSGAAARTDAAPRTPLPAGGGIPPLNLRPAANAAGAPAAAG
                     PSLHVVLTVMADPGARFTGMELLMAAHDLGLKFSKQGLFDGYVNGEVRGKPVFSIANV
                     IEPGVFPPGEVQQLSTPGLLLFMKLPGSVAEPLPSLELMLSQARRLAAKLNGTLCDEQ
                     RRRLGPDGISELRLRVTDFQRRLRQTAPA"
BASE COUNT          835 a         2314 c         2315 g          855 t
ORIGIN      
        1 acgcatcagc ccgcgcagga aggccgggtt gcggtcacgc ttggcggcgt gatcgagcgt
       61 gcgcgccagc tgcagccggt ggacgcggat cgggcggaag cgccccgggg cgagttcgcc
      121 gctcgcgagc aggcggttca ccagctcgat cgtgccgagc gcctgctcca gcgccagatg
      181 gccggcgagc tcgccgtggc ggtcgcggat cgcatcgagc gtgcgcggca gctccggcgt
      241 gctgcacggg ttgatctgca cgacccacag ctcgtcgggc gcgagcgcgg cgagttcgcg
      301 gatcggcggg ttctgcgtga acaggccgtc ccagtaatga ccggccccga cgcgcacggc
      361 acggaacagc gtcggggtcg ccgtcgaggc gagcagcgtc gccaccgtga tctcggcgtc
      421 gcggaagctg cgtgcgcagc cggcgagcac gtcgacggcg ccgacgaaca ggctcggccg
      481 gccggggccg gtgagctgcg ggatgcgctc gaactcgacg tgccattcga gcagcgaacg
      541 cagctggtcc tggccccagt tcggcacgtc gtacggcgac agctccggca gcagcaggtg
      601 atcgcgcagc cgcgcgctgc cgagcacgaa ctcgttgagc agctgatccg gcagcgtctc
      661 ggcggcgttg tcgtgccaga agtcctccag caggcgcacg ccctcggccg gcgagccgag
      721 cagcagcccg taccaggcga gcagcgcgca gatcgccccg cccgagctgc cggaaaaccc
      781 ggcgatcgtg aagcgcccgt ccgccccggc cgccgccagc agctgctgca gcacgccggc
      841 cgtgaacgcc gtgtgactgc cgccgccctg gcaggcgatc gcgatgcgcg gcagccgcgc
      901 cgcacccgcc tcccccgcat cggcgcagcc gcccattgct tcaggccacg cgcgccgacc
      961 agctccagcg cccggcgagg cgcagctcca gccggtcgcc ggacgcgagc ggtccgacgc
     1021 cggccggcgt gccggtcagc accacgtcgc cgggcagcag cgtgaagtgg cgcgagatgt
     1081 gggcgatcag gtcgaggatc ggatgcatca tctgcgcggt gctgccgtgc tggcggcgct
     1141 cgccgttgac cagcagttcg agttccgccg cctgcggctc cggcagttcc tcggggcgca
     1201 cgaagcccga cagcggacag gcgccgtcga aggcctttgc gagttcccag ggctggccct
     1261 tgttcttcag ctcggtctgc acctcgcgca gcgtcaggtc gagcgcgagc ccgtaaccgg
     1321 cgatcgcctc gcgcgccgcc gccgggctgg tgccggtcag gcgcctgccg atcagcacgg
     1381 cgagttccag ctcgtgatgg cagctgccgc gcgcctcggg caggcgcagc ggcgcatcga
     1441 gaccgacgat cgccgtcgag ggtttcatga acagcagcgg cgcctcgggc acggcgttgc
     1501 cgagttcgcg cacgtggtcg aggtagttgc ggccgatgca gacgatcttg ccgaccggca
     1561 ggtcgatcgg ctgaccgtca cggtcgcggt gctggtaggc atgttccatc gtcagggctc
     1621 ccggtccgcg caccggcagc ggcggacgac tgacagatcg gcggaattgc tgcggttcgc
     1681 aggatgatcc cgccatcgct cgcgacggcg gctgccggca tttaagccgc tcgccggtgc
     1741 cgcgacaacg ctgcacgatg ctgcgccgca atgagcgtgc aagggctggt ttcggcctgc
     1801 cggcttgcga tgacttcgcc ggtgccggcc cgcacaatgc cgcacccgcc cgcccggacc
     1861 gcccgccatg ccgatgctcg acctgcccga ctgccgcctg cattaccgcg agaccggcag
     1921 cggtgccccg ctgctgctgc tgcacggcaa cttcgcgagc cagcagtggt gggccccgca
     1981 gttcgcggac ccgccggccg ggcgccggct gatcgccccc gacctgcgcg gcctcggcga
     2041 ttccgggcga ccgggccacg gctacgacgt gccgtgcgcc gccgccgacg tgctggcgct
     2101 gctcgatgcg ctcgacatcg aacgctgcga cgtggtcggg cactcgctcg gcggcgtggt
     2161 cgcgctgcag ctcgcgatcc tgcagccgca gcggatccgc agcctgctgc tgctgacgcc
     2221 ggcgccggcc gacggcatcc ggctcgatca cctcggcccg ctggcctggc tcggcgccga
     2281 gcgcatcgcc cgcctgatcc cgacgctgac ccagcaccac gcgacgatgg atcacctgct
     2341 gccgcacgcg ctgccgggcc tgcgcgagga cgccgcccag atcgcgaagc tctgcgagat
     2401 cgcgctcgcc tgcgacccgg cggcgatcgc cggctacacc ggcgcgctcg ccggcctgcg
     2461 cctgctgccg gcgctcgcga cgctcgatca gccggtgctg gtgctcgccg gggcgctcga
     2521 tccgctggtg tccccgcagt cgctggaacc gacggtcgcg cagcttcgcc atggcgccct
     2581 gctgatctgg tccgactgcg gtcacgcacc gcagctcgaa caggtgcagc cgtttcgcac
     2641 cctgctcgcc gactggctgg ccgatgcctc ggcggtgctc gccgacatcg gcctgcacgg
     2701 cgtggtgaat ccgccgccga tcgtcattga accgccaccg ccggagcaac tgctggaaac
     2761 gacggttccg ctgacgctgc cggcgctgca gccggccgcc aacgagctgc tgcaatcggc
     2821 gttcgaggcc gcgcagcagg ccactcaggc cggcaccgcc gcctcggtcg ccgcggtcga
     2881 gacggccgtg caggcccagg ccgggctgct gcagcggctg cgcagctggc tgcacctcgg
     2941 cgactgatac cgctcacccg atcggcatca acctccgccc gccgactccc actgcgccag
     3001 ccgctgcagg aacgccgcct cgtcgagcac ctcgacgccg agttcctgcg ccttcgcgag
     3061 cttgctgccg gcctcggcgc cggcgacgac atagccggtc ttcttcgaga cgctgccggc
     3121 gaccttcgcg ccgagggctt cgaggcgggc cctggcctcg tcgcgggtca tcgccgacaa
     3181 ggtgccggtc agcacgacgg tgaggccatc gagcggccgg acgatgagcg gcgcggcggg
     3241 cagcagcgcg agcagtccgg ccgcgaaacc ggcggtgcgc tgcacgcgcg cgcgggattc
     3301 cggcacggca agccagtcgc gcagcgcctg tacgtgcccg gccggcagtc cggtgcctgc
     3361 gaggcgcgcc tcatcggcga gcaggtccgg cagcgcggcc agcccctgcg ccccgagcgc
     3421 cggtgcgagc tggcgggcgc gggcttcact gagcttcggg gcgccgagcg cggcgagcag
     3481 cgcgaccggg ttcagacgtt cggcgagcgc ggcggccggc ggatgttcgt cggcgggctg
     3541 cacgccgccg gcgagcagcg catcgagcgc ctgctggttg ccgggctgaa ggaagaactc
     3601 gtcgatggcc tcggcgacct cgggaccgat gtcgggcagc gcgcgcagca ccggtgccgg
     3661 cgcgtgacgc acgcgttcga gcgtaccgag ccagtcggcg agcgtctttg cggtcgattc
     3721 gccgacgtgg cggatgccga gtgcgaacag caggcgcgcg agcgtcgtgt gccggctgcg
     3781 cgccagggcc ccgatcaggt tctcggccca cttcgtcgcg atcctgccct gcgcgacggt
     3841 ctcgggcgtg atgccgtcgc gctcgtcggc gcggcgcttc atctccagga agtcatcgag
     3901 tgtcagccgg tacaggtcgg cgacgccgtg cacgtagccg agatcgacga ggttctcgat
     3961 gtagcgctcg ccgagcccgt cgatgtcgag cgcgcgacgc gcggcgaagt ggcggatcgc
     4021 ctccttgcgc tgcgcggcac aggtcaggcc gccgctgcag cgcgcgatcg cctcgccttc
     4081 gggccgctcg atgtgcgagc cgcacaccgg acagtgctgc ggcggctgcc agggctcgtg
     4141 ctcgggatcg aggaggctgc gcatcggccg gcgatcgagc agcacgccgg cgacctcggg
     4201 gatcacgtcg ccggcgcggc gcacgatgac ggtgtcgccg atgcgcacgt ccttgcgcgc
     4261 gacctcgtcg aagttgtgca gcgtggcatt ggtgacggtg acgccgccga cgaacaccgg
     4321 ctcgagccgc gccaccggcg tgatcgcgcc ggtgcgcccg acctgcacgt cgatggcctc
     4381 gaccgtggtg agctgctcct gcgccgggta cttgtgcgcg ctcgcccagc gcggcgcgcg
     4441 cgcgacgaag ccgagccggc gctgccagtc aaggcgatcg accttgtaga cgacaccgtc
     4501 gatgtcgtag gcaagcgctg cgcgctgcac gccgatgccg gcgtaataac cgagacagcc
     4561 gtccgggccg aagaccaccg cacgctgcgg gcagaccggc agctcccagt cgccgagccg
     4621 ctcgatgatc gccgagtgct cgccgggcag cgcgcgctcg tccgagacct cgccgagacc
     4681 gtaggcgtag aacgacagcc gccgcccggc ggtcaccgcc gggtcgagct ggcgcaggct
     4741 gccggcggcg gcgttgcgcg ggttcgcgaa ggtcttgtcg ccgcgttcgc gggcgcgctc
     4801 gttcaggcgc tcgaagtcgc ggcgcagcat gaacacctcg ccgcgcacct ccagcacctc
     4861 cggccagccg ccgccggcga ggcgcagcgg gatcgcgcgg atcgtgcgca cgttggcggt
     4921 gatgtcctcg ccggtcgtgc cgtcgccgcg ggtcgcggcc tgacggagca ggccgtgctc
     4981 gtagcgcagg ctgacggcga ggccgtcgag cttcggctcg gccgaatagg cgatcgcatc
     5041 cacctcgatg ccggccgcgg ccagccgctc gcgcgcgcgg cggtcgaagt cgcgcacgtc
     5101 ggcctcggtg aaggcgttgt cgagcgacag catcggcagc gcgtggcgca ccggcgcaaa
     5161 gcccgccgcc ggcctgccgc cgacgcgctg gctcggcgaa tccggcgtga tccactgcgg
     5221 atgcccgtcc tcgatgccgc gcagttcgat gaccagcgca tcgaacaccg catcggggac
     5281 ttcgggctgg tcgagcacgt agtacaggta ctcgtgccgg cgaatctcgt cgcgcagctg
     5341 cagcagccgg gcgtggggat cgaggtggct catgcgggtc acgcgcagca ggtcatgccg
     5401 gtctcgcggc tgaacacccg gtcttttcag cccctccccg ctcgcggggg aggcctgggg
     5461 agcggggatg aaccagggcg ttcatgcgtg atcggcagga aggttttccc gccgtccccc
     5521 tctccgcctc gacgccgggc gggggtcggc agggtctgcc ggtcgggcga tcgcgacgac
     5581 cggtgcggag aggggctgga aagcaggccg ggtgacaggg agcgggcgcg cgattgatcc
     5641 gccccgcccg gccgttcacc gccgtcgttc aggcgcccgc cgcctgcggg agtgcagcag
     5701 cgggcagcgg gaccgccatg gccgacggcg ccggagaccg ccggcagctc aggccggtgc
     5761 cgtctgccgc agccggcgct ggaagtcggt cacgcgcagg cgcagctcgc tgatgccgtc
     5821 cgggccgagc cggcgacgct gctcgtcgca cagcgtgccg ttgagcttgg ccgccagccg
     5881 gcgcgcctgc gacagcatca gctccagcga cggcagcggc tcggcgaccg atcccggcag
     5941 cttcatgaac agcagcaggc ccggcgtgct gagctgctgc acctcgccgg gcgggaacac
     6001 gcccggctcg atgacgttgg cgatcgagaa caccggcttg ccgcgcacct cgccgttgac
     6061 gtagccgtcg aacaggccct gcttgctgaa cttcagcccc agatcgtgcg cagccatcag
     6121 cagctccatg ccggtgaagc gcgcgccggg atcggccatc accgtcagca cgacgtgcag
     6181 gctcggccct gccgccgccg gcgcaccggc ggcgttggcg gccgggcgca ggttcagcgg
     6241 cgggatgccg ccaccggccg gcagcggcgt gcgcggcgcg gcatcggtgc gcgccgcggc
     6301 accgctccag gtcatgccc
//
LOCUS       DAOQIE010000238         6310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6704_length_6310_cov_6.772982, whole genome shotgun sequence.
ACCESSION   DAOQIE010000238 DAOQIE010000000
VERSION     DAOQIE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6704_length_6310_cov_6.772982"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..587)
                     /locus_tag="PK144_13710"
     CDS             complement(273..587)
                     /locus_tag="PK144_13710"
                     /inference="COORDINATES: protein motif:HMM:NF026905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HNF66829.1"
                     /translation="MVIKLPVAEDECMPGLNRESATLLARGRIDLLAQAAGDEFEMLL
                     DFTREIEQGWVFFYNTADFVRTQNPVSALAGNGPILVTREGVVHELPSAIPWEDSAHG
                     LD"
     gene            939..1796
                     /locus_tag="PK144_13715"
     CDS             939..1796
                     /locus_tag="PK144_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF66830.1"
                     /translation="MSTPHPLIDAAALAARLDDPDLVVIDCRFNLMDKAAGPRAWAAA
                     HLPGARYADLDADLAGPVTGTSGRHPLPAPAAFAASLSRWGVRPDSFVVAYDAAGGAM
                     AAARLWWLLRWVGHGRAAVLDGGLPDWQRRGLPLTDAAPAVAASDYPVQPDDARWLGI
                     AALKAALAAGESLVDARAAARYRGETEPVDPVAGHVPGALNLPQDGNLDAEGRFLPPA
                     ALAARFGAVLGERAPARVIHMCGSGVTACHNLLAMEAAGLTGSKLYAGSWSEWIRDPA
                     NPVATGAEP"
     gene            1990..2706
                     /locus_tag="PK144_13720"
     CDS             1990..2706
                     /locus_tag="PK144_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF66831.1"
                     /translation="MRFMHASFLKTGSIFGLIAALAFLATGCVQPASVAPPAGTQTAC
                     GFVNTAETNNTATGALIGAVAGAALGAATGHSKGKRALIGAAGGAVVGGAIGAYMDNK
                     EQALRQQLAGTGVIVSRSGNIVILTFPEGITFPVGKSTLSSGGRSALGRIAPTLASAD
                     RTRIGVCGHTDNTGSRELNERLSYDRATVVANTLSGFGVPSGRMIVRGFAYDMPIASN
                     ATPEGRARNRRVEIVLEPVN"
     gene            2779..3537
                     /locus_tag="PK144_13725"
     CDS             2779..3537
                     /locus_tag="PK144_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNF66832.1"
                     /translation="MCLILFAYRVHPDYPLILAANRDEFYARPALPLGWWQDAPDLLA
                     GRDLAAGGTWAGITRSGRFAAITNYRDPARMRADAPSRGALVGDFLRGTQTAPAYLDD
                     VAAAGLPYNGFNLLAADPAAGLWYYGSRDGRPRAVEAGVHGLSNALLDTPWPKVRRGK
                     AGLQPLLEAGTRPDPEALFTLLADTSRPPDAELPSTGVSPEWERLLSPAWIVSPTYGT
                     RGSLVLLWRADGRVEMIEREAGSGEERAFRLRVG"
     gene            4057..>5057
                     /locus_tag="PK144_13730"
     CDS             4057..>5057
                     /locus_tag="PK144_13730"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HNF66833.1"
                     /translation="MTQYELVPSSSSSEGEGNDFVFLSESEPLETLASSANTDPDLWR
                     VLAVDDDPDFQRSMAFALSGMCVLGRKIELLQAGSHAEAAQILAREQDIAVMLLDVVM
                     ETDDAGLRLARAVREVLGNAELRIVLVTGQPGFAPVREVMEHYDINDYWSKSELGIER
                     LHTLLTANTRAWMQVHTIARARRGLQMIVEASNVLVGARNLNDFSARTIAEIASVLGV
                     APEGVVCVRRTRNEVCIIGAAGRYAGTVGKSIDSIATARVHEAISTCLREQRTLIRAD
                     CMVLYFPSSLAGSEYVAWVEIENELDATEQELLRVFAMNIGGGLHNVALFSRLDELA"
     assembly_gap    5058..5157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5158..6191
                     /locus_tag="PK144_13735"
     CDS             <5158..6191
                     /locus_tag="PK144_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66834.1"
                     /translation="FALLGPSHLIDPAEVRRKLADDLDAVKDGGTCTFGSAALPLARF
                     EGNAGEALTTAMLLMRNAKRRGHGQHQDWIPSLAEEAGSRFELLNQFRDALESDGFTI
                     ELQPQVNLTTGAVVGVEALARWRRADGSRVPPDVFIGLAETTGLIQPLSERIYAQAFE
                     AADQLAEAGFAHVHVAINVSARQFMSNGQLPKFQNHLGAKMISAKRIEVEITESTAME
                     NYSVIRGTLESLRRCGHTVAIDDFGTGYSSLACLRNLPADLLKIDRSFVQEIGGEADQ
                     TAIADLVIRLGQRLGLNTIAEGVETPAQAGWLRARGCNLAQGWLFSRPLPLDVLIPWL
                     EKGQGRVQV"
BASE COUNT         1038 a         2000 c         2044 g         1128 t
ORIGIN      
        1 cagccgcttc atccagcagg catccagcac gtaatctgga tggatcaagg aaacccggca
       61 gtcatcgaat cgaataatgc cgagcacacc ggaacgctgc gcttttcagc cttttgcccc
      121 tcgtggggga gagcttgggg agagggagaa aaaccatgca gctacgtgtg atcggtatga
      181 gagagtcccg aaggcgcaca tctgttcgat cgtttggtca ttccgttttc tcatgctgct
      241 ccctgtctgt tcccaatgct gggtttcgtt cctcaatcca gcccgtgcgc tgaatcttcc
      301 caaggaatcg cgctaggcaa ttcgtggaca acaccttcac gggtaacaag aatcgggccg
      361 ttacccgcaa gggcagatac gggattttgt gtccgcacga aatctgcagt gttatagaag
      421 aatacccatc cctgctcgat ctcccttgtg aagtcgagca acatctcgaa ttcatcccct
      481 gccgcctgag caagcaggtc aatcctgcca cgagcaagca aggtagcaga ttcacggttg
      541 aggccaggca tacattcgtc ctcggcaact ggaagcttaa taaccattga atttcagtgt
      601 ctggtcccgg gtggtcatca aaacctggat gggcgaacct tcttgtcatc gccaatcatc
      661 gcttttaggg gattcgtaga cgagatcgcc ggaaaggcaa cggctggttc tgatgtcttg
      721 cggggcttga tgcgcgctga gcggaagtcg ggcggcgcac acacaggtat cgggttgatt
      781 tgacgtgcgg ccatgcgctg ccggcgcagt ttccgcggcg gccgtcagca cgggactcgc
      841 ctgggtgctg tgccgggtcc gcgccggcgg accgcgcgca tcggccgcgc aggctataga
      901 gtgcgctcgc cttccgagcc gcaggagatt tccgcccgat gagcaccccg cacccgctga
      961 tcgacgccgc cgcgctcgcg gcgcgcctcg atgatcccga tctcgtcgtc atcgactgcc
     1021 gcttcaacct gatggacaag gccgccggcc cccgggcctg ggccgcggca cacctgcccg
     1081 gtgcccggta cgccgatctc gatgccgacc ttgccggccc ggtcacgggc acgagcggcc
     1141 gccatccgct gccggcgccg gcggcgttcg ccgcctcgct gtcgcgctgg ggcgtgcgcc
     1201 cggacagctt cgtcgtcgcc tacgacgcgg ccggcggtgc gatggccgcg gcccggctgt
     1261 ggtggctgct gcgctgggtc gggcacgggc gggctgcggt gctcgatggc ggcctgccgg
     1321 actggcagcg ccgcggtctg ccgctgaccg acgccgcgcc cgcggtcgcg gccagcgact
     1381 acccggtgca gcccgacgat gcgcgctggc tcggcatcgc ggcgctgaag gccgcactcg
     1441 cggccggcga gtcgctggtc gatgcacgcg ccgcagcgcg ctatcgcggc gagaccgagc
     1501 cggtcgatcc ggtcgccggc cacgtgcccg gggcgctgaa cctgccccag gacggcaacc
     1561 tcgacgccga aggccgcttc ctgccgccgg cggcgctggc cgcgcgcttc ggcgccgtgc
     1621 tcggcgagcg cgccccggcg cgcgtgatcc acatgtgcgg ctccggcgtc accgcctgtc
     1681 acaatctgct ggcgatggag gctgcgggcc tcaccggctc gaagctctac gccggctcct
     1741 ggagcgaatg gatccgcgat ccggccaacc cggtcgcgac cggcgccgaa ccctgagcat
     1801 cgccggcgca gtcgatcgga gtccatatcg catcgcccgg cacggtctgc acgggcggaa
     1861 tgcgcaatcc cggacccgat gctgcggctc cgctcctgcg ccgctgccgg cgtcatctac
     1921 gctggggccg gctggctgaa accgcctcac cacaatgacg aatgtttctt cacgggagac
     1981 ccgcccatca tgcgtttcat gcatgcgtcc tttctgaaaa ccggctccat cttcggcctg
     2041 atcgccgcgc tggcgtttct cgccaccggc tgcgtgcagc cggcctcggt cgcgccgccg
     2101 gccggcacgc agaccgcctg cggcttcgtc aacaccgccg agaccaacaa caccgcgacc
     2161 ggcgcgctga tcggtgcggt cgccggtgcc gcgctcgggg ccgcgaccgg ccacagcaag
     2221 ggcaagcggg cgctgatcgg tgcggccggc ggtgccgtgg tcggcggcgc gatcggcgcc
     2281 tacatggaca acaaggagca ggcactgcgc cagcagctcg ccggcaccgg cgtcatcgtc
     2341 tcgcgctccg gcaacatcgt catcctgacc ttcccggaag gcatcacctt cccggtcggc
     2401 aagtcgacgc tgagcagcgg cggccgttcc gcgctcgggc gcatcgcgcc gacgctggcc
     2461 tcggccgacc gcacgcgcat cggcgtctgc ggccacaccg acaacaccgg ctcgcgcgag
     2521 ctgaacgagc gtctgtcgta tgaccgcgcc actgtcgttg ccaacacgct gagcggtttc
     2581 ggcgtgccct cgggccgcat gatcgtgcgc ggcttcgcct acgacatgcc gatcgccagc
     2641 aacgccacgc cggaaggccg cgcgcgcaac cgtcgcgtcg agatcgtgct ggagccggtc
     2701 aactgacggt cacggcggcg ggcacggacg gcgggtatat gctcggcgcc cgtcctgccc
     2761 gctgccggag cgacgtccgt gtgcctgatc ctgttcgcgt accgcgtcca tcctgactat
     2821 ccgctgatcc tcgccgccaa ccgcgacgag ttctacgccc gcccggccct gccgctcggc
     2881 tggtggcagg atgccccgga tctgctcgcc gggcgtgatc tcgccgccgg cggcacctgg
     2941 gccggcatca cccgcagcgg ccgcttcgcc gcgatcacca attaccgcga tcccgcccgg
     3001 atgcgcgccg atgcgccgtc gcgcggcgcg ctggtcggcg atttcctgcg cggcacgcag
     3061 acggccccgg cctatctcga cgacgtggcc gccgccggcc tgccgtacaa cggcttcaac
     3121 ctgctcgccg ccgatccggc cgccgggctc tggtactacg gcagccgcga cggcaggccg
     3181 cgcgccgtgg aagccggcgt gcacggcctg agcaacgccc tgctcgatac gccgtggccg
     3241 aaggtgcgcc gcggcaaggc gggcctgcag cccctgctcg aagccggcac ccgcccggac
     3301 ccggaagccc tgttcacgct gcttgccgac accagccgcc cgcccgacgc cgaactgccc
     3361 tcgaccggcg tgtcgcccga atgggaacgc ctgctgtctc cggcctggat cgtcagcccg
     3421 acctacggca cacgcggctc gctggtgctg ctgtggcggg ccgatggcag ggtcgagatg
     3481 atcgagcgcg aggccggcag cggcgaggag cgggcgttcc ggctgcgggt ggggtgacac
     3541 ccggcaccgc gctgatgagc cctgcccggc ggcggcgcgc cggcgtgcgg tgaccggtga
     3601 aaccgagcgc ctgcaccctc tccggggtca ttgcaatgaa gcgcacgggg ccggcggtaa
     3661 gccggcatcg ctcaggcgtg tcgtgcggac ttcctgaaaa gatcgctgaa agccatgtcc
     3721 tgaacgggca gatgcggtaa ttcgttcgag gccgtcaggt tgttgacgat ttgttgacgg
     3781 tgcgaggtgt ttcaatgaca ggctgaataa gtgtcaaaaa aatgccattt ccagattgct
     3841 tacctataaa tcgtgaatca ttactgctgc agttgtcgta ttgaaaatac tatatatttg
     3901 gtaatgattg aacctgcatc tcaaggatca aaggtgattg aacgcatgaa tcacgccaga
     3961 ttcatggaaa atgtccggga tggatcagaa ttgctttcag gcattctgcc agtgcggagt
     4021 tgatgggcgc tacaagcatg caattgtgag gcaatcatga cgcaatacga actggtgccc
     4081 tcttcatcgt cgtcggaagg ggaagggaat gattttgtct tcctgtcaga atcagagccg
     4141 ctggagacgc tggcttccag tgccaacacg gatcctgatc tctggcgggt gctggctgtc
     4201 gatgatgacc ccgacttcca gcgctccatg gccttcgctc tttccggcat gtgcgtgctc
     4261 ggcaggaaga ttgaactgct gcaggccggc agccatgccg aggccgcaca aatcctggcc
     4321 agggagcagg acattgcggt gatgctgctc gatgtggtca tggaaacgga cgacgcgggt
     4381 ctcaggctgg cccgggcggt ccgcgaagtg ctcgggaatg ccgagctgcg catcgtgctc
     4441 gtgaccgggc agccgggttt tgcgccggta cgtgaggtca tggagcacta tgacatcaat
     4501 gactactggt cgaagtcgga gctcggaatc gagcgtctgc acacgctgct gaccgccaac
     4561 acccgcgcct ggatgcaggt gcatacgatc gcccgtgccc gtcgtggcct gcagatgatc
     4621 gtcgaggcca gcaatgtgct ggtcggtgca cggaatctga acgatttctc ggcccgcacg
     4681 atcgccgaga ttgcctccgt gctcggcgtc gcaccggagg gcgtcgtctg tgtgcggcgc
     4741 acccggaacg aggtctgcat catcggggcc gccggccgct atgccggcac ggtcgggaaa
     4801 tccatcgatt cgattgcaac ggcccgcgtg catgaggcca tctccacctg tctgcgggag
     4861 cagcgcacgc tgatccgggc agactgcatg gtgctgtatt tcccttcttc cctggccggt
     4921 tccgagtacg tggcctgggt ggagatcgag aacgaactgg atgcaaccga gcaggagctg
     4981 ttgcgtgtct tcgccatgaa cattggcgga ggtctgcaca atgtcgcctt gttcagtcgt
     5041 cttgacgaac tggcctannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
     5161 ttgcactgct tggccccagc cacctgatcg acccggcgga agtgcgtcgc aagctcgcgg
     5221 atgatctcga tgcagtgaag gacggcggca cctgcacctt tggcagcgca gcattgccgc
     5281 tggccaggtt cgagggcaat gccggcgagg cgctgacgac cgccatgctg ctgatgcgga
     5341 atgccaagcg gcgcgggcat gggcagcatc aggactggat tccctcgctg gccgaggagg
     5401 ccggcagcag gttcgaactg ctgaaccagt ttcgcgacgc gctggaaagt gacggcttca
     5461 cgattgaact gcaaccgcag gtcaacctga ccaccggggc agtggtgggg gtggaggcgc
     5521 tggcccgctg gcgccgggcg gatggcagcc gtgttccgcc ggatgttttt attggcctgg
     5581 ctgaaacgac aggcctgata cagcctttga gcgagcgcat ttacgcgcag gcgtttgaag
     5641 cggctgatca acttgccgag gccggatttg ctcatgttca tgttgcgata aatgtatcgg
     5701 caagacagtt tatgagcaat ggccagttgc cgaaattcca gaatcatctc ggcgccaaga
     5761 tgatttccgc gaagcggatc gaagttgaaa tcacggaaag taccgcgatg gaaaattatt
     5821 ccgtaatccg ggggacgctg gagagcctgc gccgttgcgg acatacggtg gcaatagatg
     5881 attttggcac cggttattct tccctggcct gcctgcggaa tctgcctgcc gatctgctga
     5941 agattgacag gagcttcgtg caggaaatcg gtggcgaggc ggatcagact gccattgctg
     6001 atctggtgat tcggcttggt cagagactgg gcctgaatac gattgccgaa ggagtggaga
     6061 cgccggcaca ggccggctgg ctgcgggcac gcggttgtaa tctggcccag ggctggctgt
     6121 tttcccgccc tctgccgctt gatgtattga ttccgtggct ggaaaagggg cagggcagag
     6181 tccaggtctg atgcgttgag cccggagccc ggttccgtcc ggatccggaa tacgccgcgg
     6241 ctccggggtc tgccaaaccg ccgccgtgcc cggagggcat gatccggagc gaagcgccgt
     6301 gtcatccttc
//
LOCUS       DAOQIE010000239         6225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6861_length_6225_cov_7.890438, whole genome shotgun sequence.
ACCESSION   DAOQIE010000239 DAOQIE010000000
VERSION     DAOQIE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6861_length_6225_cov_7.890438"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..965
                     /locus_tag="PK144_13740"
     CDS             78..965
                     /locus_tag="PK144_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF66835.1"
                     /translation="MHPHRQAVFCALGAVLAWSTVAAAFKYSLRWLTPVELLLVASLS
                     SLLVLAGVLAVQGRLGQLRRLPARDWLASAGYGFLNPFLYYLVLFEAYRRLPAQVAQP
                     LNYAWPLVLVLLSALWLRQRLRLATLAAMGLSLAGIVLISMQGEAAGSAGSDPAGVAL
                     ALASTLIWAGYWLANSRDRQDAVLRLSANFACGTLFIALWWLAGSPRALPLPGVLGAA
                     YVGVFEMGLTFVLWLSALRLARNAAQVGGLVYLSPFLSLLFIHWVLGETIRPATVAGL
                     ALIVGGVLLNQLAGRRAVP"
     gene            997..1950
                     /locus_tag="PK144_13745"
     CDS             997..1950
                     /locus_tag="PK144_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNF66836.1"
                     /translation="MQVFEAAQVREALDAPGLIEALRAAFREGAEVPLRHHHTLAVPG
                     EPAATLLLMPAWTAGGFLGVKIAGVFPGNAARGQPAVQAQYLLMNAGTGEPLALLDGN
                     ELTARRTAAASALAAGYLARRDAARLLVVATGRLAVPLACAHAGVRDYAQILVWGRNP
                     GKAADVAGQLRRLGLPADAAVTLEAAVRSADTISCATLSTGALIRGDWLAPGTHLDLV
                     GGYTPAMREADDAAIARAEVFVDTRAGALVEAGDLVQPLASGVLRPEAVLAELAGLCR
                     GEHPGRRDAAAITVFKSVGTALEDLAAAVLVWQRRALPGCG"
     gene            2401..3531
                     /locus_tag="PK144_13750"
     CDS             2401..3531
                     /locus_tag="PK144_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNF66837.1"
                     /translation="MKKAIQRAFAVSLLAGAVAFGVGAHAADVIKIGVAGPMTGGNAA
                     FGEQFWRGAVQAAEDINAAGGVNGKKIELVKADDACEPKQAVAVANRLVDQDKVDAII
                     GHFCSSSTIPASEIYAEAGKLQITPASTNPKVTERNIPTIFRTCGRDDQQGAVAADFI
                     LSTLKAKKIAVIHDKDTYGQGLADAMKASAAKKGAKEVLYEGLTRGEKDFNALATKIK
                     AADADAVYFGGLAAEAGPLVRQLREQGSTAAFISGDGIVDTAFVTAAGGPQFSKGVYM
                     TFGADPRNLPGGKSLVEKFRKAGYEPEGYTLYSYATLQAVTEAMKATKSTDGATLAKW
                     LKGHTVNTVMGPKAWDAKGDLKVSDYVMYQWDDKGGYAELKK"
     gene            3661..4578
                     /locus_tag="PK144_13755"
     CDS             3661..4578
                     /locus_tag="PK144_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013797474.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HNF66838.1"
                     /translation="MDWHILAQQLVNGLTLGSIYGLIAIGYTMVYGIIGMINFAHGDV
                     YMVSAYLTAIAIAVLTGFGIDSAPFAIVITLALTMFITGVYGWSIERVAYRPLRGSTR
                     LAPLISAIGISLVLQNYVQLSQGAKPQGVPTLVSGTVRLGTETNFVQMTYIQILIVVV
                     SFAAMAALTWIIRSTSLGRQCRATQQDRKMAAILGIDTDRIISTVFVIGAAMAAIAGV
                     LVTLNYGSFDFFIGFVTGIKAFTAAVLGGIGSLPGAMLGGILLGLFESLFSGFVNTDY
                     KDVFSFALLVLVLIFRPSGLLGRPEVEKV"
     gene            4583..5914
                     /gene="livM"
                     /locus_tag="PK144_13760"
     CDS             4583..5914
                     /gene="livM"
                     /locus_tag="PK144_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNF66839.1"
                     /translation="MLPAAPLHSRIDVLASLREALLAFVLALVVFGPVSGLMLSGYKV
                     EPHWLRPLLLAAVVAAGRFAIHLLGQTPAGRRMVARNQARVHGGVTVAALGQSPNRAR
                     LLLVFVLLGAFVIPFLPWLPVPDALAALGGKYWLSNLILALIYVLLGLGLNIVVGLAG
                     LLDLGFVAFYAVGAYFLALGAQYLGIGFWTALVCAPLLAACCGAVLGFPVLRMHGDYL
                     AIVTLGFGEIIRLVLNNWLEFTGGPNGAPVPPPTLFGLEFTRTARQGGTPIHEFLGIE
                     YNTNLKLVFTYLVLLVVVILMVRLLTRLRQIPMGRAWEALREDEIACRSLGINHVVVK
                     LTAFMMGAMTGGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGLGSTVGVIIAAL
                     VLTLLPELLREFADFRVLAFGLLMVLMMIWRPRGLIRPQRPAFAPPQEVAR"
     gene            5911..>6225
                     /locus_tag="PK144_13765"
     CDS             5911..>6225
                     /locus_tag="PK144_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF66840.1"
                     /translation="MKPSSLLEVRGLTMRFGGIVANNDVSFDVADGSITALIGPNGAG
                     KTTVFNCLTGFYRASGGTIHLATAAGAPVEVGSLLDKPFIGGSYLVARAGIARTFQNI
                     RLF"
BASE COUNT          812 a         2245 c         2131 g         1037 t
ORIGIN      
        1 cccggcggtt tgccctgcgc acggggcaat gccgatactg ctgcgcaatc cctgcccgag
       61 tccgtagccc cgtccgcatg catccccatc gtcaggccgt gttctgcgcc ctcggcgccg
      121 tgctggcgtg gtccacggtc gccgcggcct tcaagtacag cctgcgctgg ctgacgccgg
      181 tcgagctgct gctggtggcc tcgctcagtt cgctgctggt gctcgccggc gtgctggccg
      241 tgcagggccg gctcgggcag ctgcgccggc tgccggcccg tgactggctg gcttcggccg
      301 gctacggctt cctgaacccc ttcctgtact acctcgtgct gttcgaggcc taccggcggc
      361 tgccggcgca ggtcgcgcag ccgctgaact acgcgtggcc gctggtgctg gtgctgctgt
      421 cggcgctgtg gctgcgccag cgcctgcggc tcgcgacgct ggcggcgatg gggctgtcgc
      481 tcgcgggcat cgtgctgatc tcgatgcagg gcgaggccgc gggcagcgcc ggcagtgacc
      541 cggccggcgt ggcgctggcg ctggcgagca cgctgatctg ggccggctac tggctcgcca
      601 acagccgcga ccggcaggat gcggtgctgc gcctgagcgc caacttcgcc tgcggcacgc
      661 tgttcatcgc gctctggtgg ctcgccggct cgccgcgggc gctgccgctg cccggcgtgc
      721 tcggcgcggc gtatgtcggc gtgttcgaga tggggctgac cttcgtgctg tggctgtcgg
      781 cgctgcggct ggcgcgcaat gccgcgcagg tcggcggact cgtgtacctg tcaccgttcc
      841 tgtcgctgct gttcatccac tgggtgctcg gcgagaccat ccggccggcg acggtcgccg
      901 gtctggcgct gatcgtcggc ggcgtgctgc tgaaccagct cgccggccgg cgcgcggtgc
      961 cctgaagttt tttcgcaacc gccgccggag gtgagcatgc aggtgttcga ggccgcacag
     1021 gtgcgcgagg cgctcgacgc gccggggctg atcgaggcgc tgcgcgcggc gtttcgcgag
     1081 ggcgccgagg tgccgctgcg ccatcaccac acgctcgcgg tacccggcga gccggcggcg
     1141 acgctgctgc tgatgccggc gtggacggcg ggcggcttcc ttggcgtcaa gatcgccggc
     1201 gtcttccccg gcaacgccgc gcgcggccag ccggcggtgc aggcgcagta cctgctgatg
     1261 aacgccggca ccggcgagcc gctggcgctg ctcgatggca acgagctgac cgcgcggcgc
     1321 acggcggccg cttcggcgct cgcggccggc tatctggcgc ggcgcgacgc cgcccggctg
     1381 ctcgtcgtcg ccaccggccg gctggcggtg ccgctcgcct gcgcacacgc gggcgtgcgc
     1441 gactacgcgc agatcttggt ctggggccgg aaccccggca aggcggcgga tgttgccggg
     1501 cagttgcgcc ggctcgggct gccggccgat gcggcggtca cgctcgaagc cgcggtgcgc
     1561 agcgccgaca cgatctcgtg cgcgacgctc agcaccgggg cgctgatccg cggtgactgg
     1621 ctcgcgcccg gcacccacct cgatctggtc ggcggctaca cgccggcgat gcgcgaggcc
     1681 gacgatgcgg cgatcgcgcg cgccgaggtc ttcgtcgaca cccgcgccgg cgcgctggtc
     1741 gaggccggcg atctggtgca gccgctcgcg agcggcgtgc tgcggcccga ggccgtgctc
     1801 gccgaactcg ccggactctg ccgcggcgag cacccgggcc ggcgcgacgc ggccgccatc
     1861 accgtgttca agtcggtcgg caccgcgctc gaggacctcg ccgcggcggt gctggtctgg
     1921 cagcggcggg cgctgccggg atgcggatga gcagcacagg actggcctga ccatcgggcc
     1981 atggcgaggc gcgccggcgg ctctctgcac cgttgcggcg cagggccggc cgcgggcggg
     2041 ggcgcggcag cggcaagttt ctgatcagcc gcgcttgcgc cgggctggca cggaaacggt
     2101 attccgcctg cggaatgtgc cgcttcgggg cgcccgtgat ctgcgtcagc caagctgtcc
     2161 ggtgtccggc ccgcaggctt gacagtcgcg tgacacggca gccataagca ctgcgcggaa
     2221 cagtggcacc cccagaacgt gccggccggc gcaggacgcc ggtaccggat tccgcccgcg
     2281 cccgcgacgg ccgcccgagg cggtcgcgac ggacgcggaa cccgccgcag cctcatcttc
     2341 cgatccagtc cattcgtcct atccaacatc ccttaccgct acttgcgtag gagcagaacg
     2401 atgaagaagg ctatccagcg cgcctttgcc gtgtccctgc tggccggtgc cgtcgccttt
     2461 ggcgtcggcg cgcacgcggc cgacgtgatc aagatcggtg ttgccggccc gatgaccggc
     2521 ggcaacgccg cgttcggcga gcagttctgg cgcggggcgg tgcaggccgc cgaggacatc
     2581 aacgccgccg gcggcgtcaa cggcaagaag atcgagctcg tgaaggccga cgacgcctgc
     2641 gagcccaagc aggcggtcgc ggtcgccaac cgcctcgtcg accaggacaa ggtcgatgcg
     2701 atcatcggtc acttctgttc ctccagcacg atcccggcct ccgagatcta cgccgaagcc
     2761 ggcaagctgc agatcacgcc ggcctcgacc aacccgaagg tcaccgagcg caacatcccg
     2821 acgatcttcc gcacctgcgg ccgtgacgac cagcagggcg ccgtcgccgc cgacttcatc
     2881 ctcagcacgc tgaaggcgaa gaagatcgcg gtgatccacg acaaggacac ctacggccag
     2941 ggcctcgccg atgcgatgaa ggcctcggcc gccaagaagg gcgccaagga agtcctctac
     3001 gagggcctga cccgcggcga gaaggacttc aacgcgctcg ccaccaagat caaggccgcc
     3061 gatgccgatg ccgtgtactt cggcggtctc gccgccgagg ccggtccgct ggtgcgccag
     3121 ctgcgcgaac agggttcgac cgcggccttc atctccggcg acggcatcgt cgacaccgcc
     3181 ttcgtcaccg ccgccggcgg cccgcagttc tcgaagggcg tgtacatgac cttcggtgcc
     3241 gacccgcgca acctcccggg cggcaagtcg ctggtcgaga agttccgcaa ggccggctac
     3301 gagcccgagg gctacacgct ctacagctac gcgaccctgc aggcggtcac cgaggcgatg
     3361 aaggccacca agtccaccga cggcgccacg ctcgcgaagt ggctgaaggg ccacaccgtc
     3421 aacaccgtca tgggcccgaa ggcctgggac gccaagggtg acctgaaggt ctccgactac
     3481 gtcatgtacc agtgggacga caagggcggc tacgccgaac tgaagaagta agcccgcgtc
     3541 cgcaacgaga ctgcgacccc cgccacccga tccggggcgg gggtcgactt ttgtcggggc
     3601 tgcgcgcgcc gtgatccgtg cgaagccgcc gcccgcgacc ttgaccgggg agatttccgg
     3661 atggactggc acatcctcgc ccagcagctc gtcaacgggc tgacgctggg ctcgatctac
     3721 ggcctgatcg ccatcggtta cacgatggtg tacggcatca tcggcatgat caatttcgcg
     3781 cacggcgacg tgtacatggt ctcggcctac ctgaccgcga tcgcgatcgc cgtgctcacc
     3841 ggcttcggca tcgactcggc gccgttcgcc atcgtcatca cgctggcgct gacgatgttc
     3901 atcaccggcg tctacggctg gtcgatcgag cgcgtcgcct accggccgct gcgcggctcg
     3961 acgcggctcg cgccgctgat ctcggcgatc ggcatctcgc tggtgctgca gaactacgtg
     4021 cagctctccc agggcgccaa gccgcagggc gtgccgacgc tggtcagcgg caccgtgcgc
     4081 ctcggcaccg aaaccaattt cgtgcagatg acgtacatcc agatcctgat cgtcgtcgtc
     4141 agcttcgcgg cgatggcggc gctgacctgg atcatccgca gcacctcgct cggccgccag
     4201 tgccgcgcca cgcagcagga ccgcaagatg gcggcgatcc tcggcatcga caccgaccgc
     4261 atcatctcca ccgtgttcgt gatcggcgca gcgatggcgg cgatcgccgg cgtgctggtg
     4321 acgctgaact acggctcctt cgacttcttc atcggcttcg tcaccggcat caaggcgttc
     4381 acggccgcgg tgctcggcgg catcggctcg ctgcccggcg ccatgctcgg cggcatcctg
     4441 ctcggcctgt tcgaatcgct gttctcgggc ttcgtcaaca ccgattacaa ggacgtgttc
     4501 tcgttcgcgc tgctggtgct ggtgctgatc ttccgcccga gcgggctgct cggtcgtccc
     4561 gaagtggaaa aggtgtgagc cgatgctgcc cgctgccccc ctccattccc gtatcgatgt
     4621 actggcgagc ctgcgcgagg cgctgctcgc cttcgtgctc gcgctcgtgg tgttcggccc
     4681 ggtctccggc ctgatgctga gcggctacaa ggttgagccg cactggctgc ggccgctgct
     4741 gctcgccgcg gtcgtcgcgg ccggacgctt cgcgatccac ctgctcggcc agaccccggc
     4801 cggccggcgc atggtcgcgc gcaatcaggc gcgcgtgcac ggcggcgtca cggtcgccgc
     4861 cctcggccag agcccgaacc gcgcccgcct gctgctggtg ttcgtgctgc tcggcgcctt
     4921 cgtgattccc ttcctgccct ggctgccggt gccggatgcg ctcgccgcgc tcggcggcaa
     4981 gtactggctg tcgaacctga tcctggcgct gatctacgtg ctgctcggcc tcggcctcaa
     5041 catcgtcgtc ggtctcgccg gcctgctcga tctcggcttc gtcgccttct acgcggtcgg
     5101 ggcctacttc ctcgcgctcg gtgcccagta cctcggcatc ggcttctgga cggcgctcgt
     5161 gtgcgcgccg ctgctcgccg cctgctgcgg cgcggtgctc ggcttcccgg tgctgcgcat
     5221 gcacggcgac tacctcgcca tcgtcacgct cggcttcggc gagatcatcc ggctggtgct
     5281 gaacaactgg ctggagttca ccggcggtcc gaacggcgcg ccggtgccgc cgccgacgct
     5341 gttcggactc gaattcacgc gcacggcgcg ccagggcggc acgccgatcc acgagttcct
     5401 cggcatcgaa tacaacacca acctgaagct ggtgttcacg tacctggtgc tgctcgtggt
     5461 cgtcatcctc atggtgcggc tgctgacgcg cctgcggcag attccgatgg gccgggcctg
     5521 ggaggcgctg cgcgaggacg agatcgcctg ccgctcgctc ggcatcaacc acgtcgtcgt
     5581 caagctcacc gccttcatga tgggtgcgat gaccggcggc ctcgccggcg tgttcttcgc
     5641 gacctatcag ggcttcgtga acccgacctc gttcacgttc ttcgaatcgg cgctgatcct
     5701 cgccatcgtc gtgctcggcg ggctcggctc gacggtcggc gtcatcatcg ccgcgctggt
     5761 gctgacgctg ctgccggagc tgctgcgcga gttcgccgac ttccgcgtgc tcgccttcgg
     5821 cctgctgatg gtgctgatga tgatctggcg tccgcgcggc ctgatccgcc cgcagcgtcc
     5881 ggccttcgcg cccccgcagg aggtggcgcg atgaagccgt ccagcctcct cgaagtccgc
     5941 ggcctgacca tgcgcttcgg cggcatcgtc gccaacaacg acgtgtcctt cgatgtcgcc
     6001 gatggctcga tcaccgcgct gatcggcccg aacggcgccg gcaagaccac cgtcttcaac
     6061 tgcctgaccg gcttctaccg cgccagcggc ggcaccatcc acctcgccac cgccgccggc
     6121 gcgccggtcg aggtcggcag cctgctcgac aagcccttca tcggcggttc gtatctggtc
     6181 gcgcgcgccg gcatcgcccg caccttccag aacatccggc tgttc
//
LOCUS       DAOQIE010000240         6213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6876_length_6213_cov_7.133647, whole genome shotgun sequence.
ACCESSION   DAOQIE010000240 DAOQIE010000000
VERSION     DAOQIE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6876_length_6213_cov_7.133647"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK144_13770"
     CDS             complement(<1..604)
                     /locus_tag="PK144_13770"
                     /inference="COORDINATES: protein motif:HMM:NF018151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNF66841.1"
                     /translation="MKRIATVGLIGLLGLLSQNGVVHAADKDKAYAPKGAATLTCEGM
                     SKINPNSTDAALVVGWFDGYVTGLNMTTPETFDIAPWQDSLLLSNLVLNACRKNPQAK
                     FMDVVQALTNDLAAKRLKTKAEVVEISEGDKKGLMYSEVLKQAQTALKDKAGFSGTAD
                     GKWGSKTRDAIKAFQKKEGLPETGLPDSLTLLRLLQPFGKP"
     gene            complement(764..1777)
                     /gene="rsgA"
                     /locus_tag="PK144_13775"
     CDS             complement(764..1777)
                     /gene="rsgA"
                     /locus_tag="PK144_13775"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HNF66842.1"
                     /translation="MSGRRLTRRQVERIRELHENRRTRARERAARAADELEDLEAAEP
                     GTVIAHYGASLIVEDAQAALHRCLVRQNLGALACGDRVLWQRARDDSGVVVALEPRRS
                     LLARPDYSGRLKPVAANLDQVLVVIAPEPDFNEYLIDRYLAAIELIGVEAGLVVNKLD
                     LCDADRRARLEARLGTYARIGYALLHASTRTDLGLAELRTRLGGRTSILVGQSGVGKS
                     SLVRALLPEREIRTQALSAATGHGQHTTTTTTLYPLPDGGSLIDSPGVRSFELAELAP
                     GELDRGFREFAPYLGHCRYGNCAHTVEPGCALLAAVGRGELDARRLDAYRQIRDAAGK
                     RPA"
     gene            complement(1774..3039)
                     /locus_tag="PK144_13780"
     CDS             complement(1774..3039)
                     /locus_tag="PK144_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF66843.1"
                     /translation="MRESTLIFLLLLSAALALQIWLALRQLRHVRAHREQVPPAFAAD
                     IPLAAHRRAADYTLERTRFGLLALAVDTLVLLGWTLGGGFAALDAAWRGFGWGETATG
                     VAVIGSAIAIGSLLVLPLKLWRVFVIEARHGFNRTTPALFASDLVKGTLLGAALGLPL
                     LALALWLMAHGGQWWWVWVWAAWSGFQLLMLWVYPVFIAPLFNRFEPLADQSLRARVE
                     ALLARCGYASGGLYVMDGSRRSGHGNAYFTGFGRARRIVFFDTLLATLDPDEIEAVLA
                     HELGHFKRRHIVSRIALSLGLALAGLGVCAWLIGQPGFFLALGVPQPSTWAGLYLFLS
                     VAGSLGILLQPPLAWYSRRHEFEADAYAAEVADGGALKRALVRLYRDNAATLTPDPLH
                     SAVYDSHPPAPQRIAALERHTTPHAGAPA"
     gene            3134..3682
                     /gene="orn"
                     /locus_tag="PK144_13785"
     CDS             3134..3682
                     /gene="orn"
                     /locus_tag="PK144_13785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNF66844.1"
                     /translation="MTPNPEHLIWIDLEMTGLDPDSDCIIEIATIVTDNALNVLAEGP
                     VFAIHRSDDVLNGMDEWNTRQHAKSGLSARVRASSISAAAAEQATLDFLAGWVPKGKS
                     PMCGNSICQDRRFLARGMPALEAWFHYRNLDVSTLKELGKRWFPERVKGFQKGNAHLA
                     LDDIRESIAELRFYRERLFVSD"
     gene            4012..4419
                     /locus_tag="PK144_13790"
     CDS             4012..4419
                     /locus_tag="PK144_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66845.1"
                     /translation="MKAHIVPAALLLACAAALAACDDGQRPATSATQTTQTTTEQKTE
                     TAKESAAAAASAAGEAVRHAGAALGAGASAAKDVTVEAAREAADKLREGYDATTQAAG
                     RAVERGREALRDEPVGTRTGTTPADDAATRTTN"
     gene            complement(4706..5521)
                     /locus_tag="PK144_13795"
     CDS             complement(4706..5521)
                     /locus_tag="PK144_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF66846.1"
                     /translation="MTDPCLLAPPAAFLAASRAPDALLLDVRDPELRAAGHLPGARAL
                     PYASYVVPRPPAMGGLPDLDTLARVLGAIGYEPGRLVLAYDEGGSGKAARLLWTLAAL
                     GHTRMALLDGGLSALQNETTTLLTREAEPVTPTTPQLAWAQGIVAERDWVVAHLCDPD
                     VLLLDTRSAGEYAGFDVRAARGGHIPGAINLDWSTAFDPGRRPRTKPIEHLRSQLLAA
                     GVDPAREIVVYCQTHHRSAHTWVVLKALGYPRVRGYAGAWSEWGNTPGLPIEP"
     gene            5809..>6213
                     /locus_tag="PK144_13800"
     CDS             5809..>6213
                     /locus_tag="PK144_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66847.1"
                     /translation="MTRNRSVLRLVLLYAVLGIAWTVFAERILLALAPDGLVLSELEA
                     WKGVVFVIFSAGFLFLAAGGLREPDADDGQDPVPRGAGGLVGASAMAGDARQRWASAG
                     APVPWSGHGRAHPLRNDGHRAPEGHRGRPRPGP"
BASE COUNT          938 a         2195 c         2246 g          834 t
ORIGIN      
        1 ccggcttgcc gaacggctgc agcaggcgca gcagcgtcag cgaatccggc aggccggttt
       61 ccggcaggcc ttccttcttc tggaaggcct tgatcgcgtc gcgggtcttg ctgccccact
      121 tgccgtcggc ggtgccggag aagccggcct tgtccttcag cgcggtctgg gcctgcttga
      181 gcacctcgga gtacatcagg cccttcttgt cgccttccga gatctcgacg acctcggcct
      241 tggtcttcag gcgcttggcg gcgagatcgt tggtcagggc ctggaccacg tccatgaact
      301 tggcctgcgg gttcttgcgg caggcgttca gcacgaggtt cgacagcagc agcgagtcct
      361 gccacggagc gatgtcgaag gtctcgggcg tggtcatgtt cagcccggtc acgtagccat
      421 cgaaccagcc gacgaccagc gcggcatcgg tggaattcgg gttgatcttg gacatgccct
      481 cgcaggtgag ggtcgcggcg cccttgggcg cgtaggcctt gtccttgtcg gcagcgtgca
      541 cgacgccgtt ctgggacagc agaccgagca ggccgatcag gccgacggtg gcgatgcgct
      601 tcatggtgtc tccgctgatg aatccgtcac tggcatccgc atgggcaggg acgcacgtag
      661 ggggccgggt ggcacgaggg ggcgggggcg acggcgagca gccgcgcggc gggccgaatg
      721 ctagcggcgg gcgctgcgct gcacaagccg cgttaccgga cggtcacgcc gggcgtttgc
      781 ctgccgcatc acggatttga cggtaggcat cgaggcgccg cgcgtcgagt tcaccgcgcc
      841 cgaccgcggc gagcagcgcg cagcccggct cgaccgtgtg cgcgcagttg ccgtagcggc
      901 agtggccgag atacggcgcg aactcgcgaa agccgcggtc gagctcgccg ggcgcgagtt
      961 cggccagctc gaagctgcgc acgcccggcg agtcgatcag gctgccgccg tcgggcagcg
     1021 ggtagagcgt cgtcgtcgtc gtcgtgtgct ggccgtggcc ggtggccgcc gacagcgcct
     1081 gcgtgcgaat ttcgcgctcg ggcaaaagcg cgcgcaccag cgaggatttg ccgacgccgg
     1141 actggccgac gaggatgctg gtgcgtccgc cgagccgggt gcgcagctcg gcgaggccga
     1201 gatcggtgcg cgtgctcgcg tgcagcagcg catagccgat gcgcgcgtag gtgccgagcc
     1261 gggcttcgag ccgggcgcgg cggtcggcat cgcagagatc gagcttgttg acgacgaggc
     1321 cggcctcgac gccgatcagc tcgatcgccg cgaggtagcg gtcgatcagg tattcgttga
     1381 agtccggctc cggcgcgatc acgaccagca cctgatcgag attggccgcg accggcttca
     1441 gacggccgct gtagtcgggc cgcgccagca gcgagcggcg cggctccagc gccacgacca
     1501 caccggaatc gtcgcgtgcg cgctgccaga gcacgcgatc gccgcaggcg agcgcgccga
     1561 ggttctggcg caccaggcag cggtgcagcg cggcttgcgc atcctcgacg atcagcgagg
     1621 cgccgtaatg ggcgatgacg gtgccgggct cggccgcttc gagatcctcg agctcgtcgg
     1681 cggcgcgtgc cgcgcgctcg cgggcgcggg tgcggcggtt ctcgtgcaac tcgcggatgc
     1741 gctcgacctg ccggcgcgtc agccgccgcc cgctcaagcc ggtgccccgg cgtgcggggt
     1801 cgtgtgccgt tcgagcgcgg cgatgcgctg cggtgccggc gggtgcgagt cgtagacggc
     1861 cgagtgcagc ggatcggggg tcagcgtcgc ggcgttgtca cggtacagcc gcaccagcgc
     1921 gcgcttgagc gccccgccgt cggcgacctc ggcggcgtag gcgtcggcct cgaactcgtg
     1981 gcggcgcgaa taccacgcga gcggcggctg cagcaggatg ccgagcgagc cggcgacgct
     2041 caggaacagg tacagcccgg cccaggtcga gggctgcggc acgccgagcg cgaggaagaa
     2101 gcccggctgc ccgatcagcc acgcgcagac cccgagcccg gcgagcgcca gcccgagcga
     2161 cagcgcgatg cgcgagacga tgtgccggcg cttgaagtgc ccgagttcgt gcgcgagcac
     2221 ggcctcgatc tcgtcggggt cgagcgtcgc gagcagcgtg tcgaagaaca cgatgcgccg
     2281 cgcacggccg aagccggtga agtaggcgtt gccgtggccg gagcggcgcg agccgtccat
     2341 cacgtacagc ccgccggagg cgtagccgca gcgcgcgagc agcgcctcga cgcgcgcgcg
     2401 cagcgactgg tcggcgagcg gctcgaagcg gttgaacagc ggcgcgatga ataccgggta
     2461 gacccacagc atcagcagct ggaagcccga ccacgccgcc cagacccaga cccaccacca
     2521 ttgcccgccg tgcgccatca gccacagcgc cagcgccagc agcggcaggc cgagcgcggc
     2581 gccaagtagc gtgcccttga ccagatcgct cgcgaacagc gccggcgtcg tgcggttgaa
     2641 gccgtggcgc gcctcgatca cgaacacccg ccagagcttc agcggcagca ccagcaggct
     2701 gccgatcgcg atcgccgagc cgatcaccgc gaccccggtc gcggtctcgc cccagccgaa
     2761 gccgcgccag gccgcatcga gcgccgcgaa gccgccgccg agcgtccagc cgagcagcac
     2821 cagcgtgtcg acggccagcg ccagcaggcc gaagcgcgtg cgttcgagcg tgtagtcggc
     2881 cgcgcgccgg tgcgcggcga gcggaatgtc ggccgcgaag gccggcggca cctgctcgcg
     2941 gtgggcgcgc acgtggcgca gctggcgcag cgccagccag atctgcagcg ccagcgccgc
     3001 ggacagcaac agcaggaaaa tcagcgtaga ctcgcgcatt cgccggaaag gccgtgtggg
     3061 acaccggtcc cgagcgtagc ccgcgccccg cgcacgcggc aaccggcccc tgcccccgcc
     3121 gagaagccgc gccatgacgc ccaatcccga gcacctgatc tggatcgatc tggagatgac
     3181 cggactcgat ccggacagcg actgcatcat cgagatcgcc acgatcgtca ccgacaacgc
     3241 gctgaacgtg ctggccgaag gcccggtgtt cgcgatccac cgctccgacg acgtgctgaa
     3301 cggcatggac gagtggaaca cgcgccagca tgcgaagtcg ggactgagcg cgcgcgtgcg
     3361 cgccagcagc atcagcgccg ccgcggccga gcaggcgacg ctcgatttcc tcgccggctg
     3421 ggtgccgaag ggcaagtcgc cgatgtgcgg caacagcatc tgccaggacc ggcgcttcct
     3481 cgcgcgcggc atgccggcgc tggaggcgtg gttccactac cgcaacctcg acgtgagcac
     3541 gctgaaggag cttggcaagc gctggttccc ggagcgcgtc aagggcttcc agaagggcaa
     3601 cgcccacttg gcgctcgatg acatccgcga atcgatcgcc gaactgcgct tctaccgcga
     3661 gcgtctgttc gtgagcgact gaacgatccg cgcccgcctg caaggcgctg tctccacggc
     3721 ggccggcccg gcaacggacc ggccgccggc gttttggcgt cgccggcagg cgacaactca
     3781 agcccttcgt gcggcacgct tccgccgcgc ccgcccggca actgtcgccg ttgcccgacg
     3841 gtttgcgcac gcccgtggcg ctccggcgcc ggcagcggac gggcattccg ccatcaagcg
     3901 cctgcaagca cctgatcgcc gagggatttc ctggacatgg cacgaaatcg cgcattcacg
     3961 gtccatgcaa ggcaggtcga gccctgccca tcctggtgac cggaacctct catgaaagcg
     4021 cacatcgtcc ccgccgccct gctgctcgcc tgcgccgccg cgctcgccgc ctgcgacgac
     4081 ggccagcgtc cggccacatc ggccacacaa accacacaaa ccaccaccga acagaaaacc
     4141 gaaaccgcga aggaatcggc tgcagccgcg gcctcggccg ccggcgaggc ggtccgccac
     4201 gccggtgccg cgctcggtgc cggtgccagc gccgcgaagg acgtgaccgt cgaagccgcg
     4261 cgcgaggccg ccgacaagct gcgcgaaggc tacgacgcga ccacgcaggc cgccggccgc
     4321 gccgtcgagc gcgggcgcga ggctctgcgt gacgagccgg tcgggacccg caccggcacg
     4381 acgccggccg atgacgcggc cacacggacg acgaactgaa agcccgcagg cctgacggac
     4441 gggtcagcct gaacgggagg tttttccccc accggcccgc atggaacgcg agccggctga
     4501 tgcgaccgga gctgacgcgc aggctgcgcg ggtcgcaacg gaaccgcgga ccggattccg
     4561 ggtgaatgag gacggttcat acgcattccc cgtgcgaggg gatggtggtc gaccgaccgg
     4621 gctgcgggtc ccggccggtc gttttttttg tgccgctccc gtcatgcgag cagagcgcag
     4681 gccggcctcg cgccgggcgg cgtgctcagg gttcgatcgg cagtcccggc gtgttgcccc
     4741 actccgacca ggcgccggca tagccgcgca cgcgcgggta gccgagtgcc ttcagcacca
     4801 cccaggtgtg cgccgagcgg tgatgcgtct ggcagtagac gacgatctcg cgcgccggat
     4861 cgacgccggc ggccagcagc tgcgagcgca ggtgctcgat cggcttggtg cgcggccgcc
     4921 ggcccggatc gaaggccgtg ctccagtcga ggttgatcgc accggggatg tggccgccgc
     4981 gcgcggcgcg cacgtcgaag ccggcgtatt cgccggccga gcgcgtatcg agcagcagca
     5041 cgtcgggatc gcagagatgc gccacgaccc agtcgcgctc ggcaacgatg ccctgcgccc
     5101 aggccagctg cggcgtcgtc ggcgtcaccg gctccgcttc gcgggtcagc agcgtcgtcg
     5161 tctcgttctg cagcgcgctc aggccgccgt cgagcagcgc catgcgcgtg tgcccgaggg
     5221 cggccagcgt ccagagcagg cgcgcggcct tgccgctgcc gccctcgtcg taggcgagca
     5281 cgagcctgcc gggttcgtag ccgatggccc cgagcacgcg cgcgagcgtg tcgagatcgg
     5341 gcaggccgcc catcgccggg ggccgcggca cgacgtagct cgcatacggc agcgcccgtg
     5401 cgccgggcag atggccggcg gcgcgcagtt ccgggtcgcg cacatcgagc agcagcgcat
     5461 cgggtgcgcg gctggcggca aggaaggcag cgggcggggc aagcaggcac ggatcggtca
     5521 cgggcaactc cggcggcggt ttctgggccg agtataactt tgcagatcgt aacggttgcg
     5581 cacccgccgg caccggatga caccgcgatg gcaccggatg gcacccgatg gcgtgcagca
     5641 actcatgaca cggccggcgc ggctgccgta tcgtcgcgcg ccatcccgcc cgtgccccgg
     5701 aatccccctg accatgtgga agcgcctgct cgactggctg ctcgtccgcg ccaccgcccc
     5761 ttccgccggt atcatggtgc gcgggtgatt tccgacccgc accgccccgt gacccgcaac
     5821 cgcagcgtcc tccgtctcgt cctcctctac gccgtcctcg ggatcgcctg gacggtcttc
     5881 gccgaacgca tcctgctggc cctggcgccg gacggcctcg tcctctccga actggaagcc
     5941 tggaaggggg tcgtcttcgt gatcttctcc gccgggttcc tgttcctggc ggcgggtggg
     6001 ctgcgggaac ccgatgccga cgacggacag gacccggttc cccggggcgc gggcgggctg
     6061 gtgggtgcgt cggccatggc gggggacgct agacaacggt gggcgtcggc gggggcgccg
     6121 gtaccctggt cgggccatgg acgagcccac cccctccgca acgacggcca tcgtgcccct
     6181 gaaggtcacc gaggccgccc tcggccaggt cct
//
LOCUS       DAOQIE010000241         6189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6912_length_6189_cov_7.360939, whole genome shotgun sequence.
ACCESSION   DAOQIE010000241 DAOQIE010000000
VERSION     DAOQIE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6912_length_6189_cov_7.360939"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="ftsZ"
                     /locus_tag="PK144_13805"
     CDS             complement(<1..1052)
                     /gene="ftsZ"
                     /locus_tag="PK144_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNF66848.1"
                     /translation="MFEIIETPHQEAIIKVIGIGGGGGNAVQHMVTSHTIEGVDFYCA
                     NTDAQALRTYSVPHAIQLGSGITKGLGAGANPEVGKKAAMEDRERVREALEGADMVFI
                     TAGMGGGTGTGGAPVVAQVAREMGILTVAVVTKPFPFEGRKRMDVAVAGIKELADEVD
                     SLITIPNEKLLAVLGKNATLLDAFKAANDVLAGAVQGIAELITRPGLINVDFADVRTV
                     MSEMGVAMMGTGRASGNGRAREAAEAAIASPLLESVDLAGARGLLVNVTAGADLTMGE
                     FHEVGEIVHALAAPEATVVIGTVIDPEMTSELRVTIVATGISSVPAAVQPAAQPAVRS
                     MASRNAGAAPADRPIAR"
     gene            complement(1195..2427)
                     /gene="ftsA"
                     /locus_tag="PK144_13810"
     CDS             complement(1195..2427)
                     /gene="ftsA"
                     /locus_tag="PK144_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNF66849.1"
                     /translation="MSRKNEKKMVVGLDIGTSKVVALVGEAAADGSMEVIGVGMHPSR
                     GLKKGVVVNIESTVQSIQHAIEEAERTAGCQIHSVHTGIAGSHIRSLNSHGIVPIRDK
                     EVTQEDIDRVIEAAGAVAIPTDQKVLHILAQEFLIDHQEGIREPIGMSGVRLEARVHM
                     VTGAVSAAQNIVKCVERCSLEVDDIVLQPLASARAVLTEDERELGVCLVDIGGGTTDL
                     AVFVNGAIQHSAVIPIAGDQVTNDIAVAFRTPTQFAESLKIQHATCLTALAPADQEID
                     VPSVGERAPRRLSMQTLAEVVEARYEELFGLVQAELRRSGFEDMLAAGIVLTGGSSRI
                     RGAVELAEEIFHVPVRAGTSLAVTGLQEVLANPACSTAVGLLLVGAEARGPVRTGPLR
                     ENRQGLFARMKAWFQGNF"
     gene            complement(2456..3352)
                     /locus_tag="PK144_13815"
     CDS             complement(2456..3352)
                     /locus_tag="PK144_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNF66850.1"
                     /translation="MSALLRRRRRRGATARVLRGDGDGAAPLSPWIGRTLRFCGTLLA
                     LGGIAWGGWRGVDWMLREGEFPLRQVHVDGDLRNLGPEDAEAAVRPYLGRNFFRLDMG
                     SLHAAFADNPWVQSVAVSRRWPDTVEVRFVERVPFAHWGRDEMIDTGAARFRPQKIRE
                     PHEWPNFDAPDGHEALLMRVYGDTGRALAPLGLKVVRVQQDARGAWTLTLDTGLELRL
                     GREAYAERLARFIEVWPRVLAAQAERIAGVDLRYLNGFAVRWKAPSAEPGTHGRTRGA
                     PIATAPATARSEDRVFTGHEAG"
     gene            complement(3349..4317)
                     /locus_tag="PK144_13820"
     CDS             complement(3349..4317)
                     /locus_tag="PK144_13820"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNF66851.1"
                     /translation="MTTKPMSDPSPHCTDPAAFGKVAVLYGGWSAEREVSLRSGATVL
                     GALRRRGVDAHGIDAGRDVTDVLRAGAYDRAFIVLHGRGGEDGVIQGALELLGIPYTG
                     SGVQASAIGMDKLRTKQIWLAEGLPVLAHRLVTCERELEGVIETLGLPLAVKPTREGS
                     SIGVSRVEHAGQLAEAFRLAAACRSPVLIEPWIVGRELTGAVLGDAALPLIRMQAAVE
                     FYDYEAKYNRDDTVYHCPAGLPEALEQSLRELCRRAYAAVGASGWGRVDLVLDAEDRP
                     WLLEVNTVPGMTDHSLVPMAARAAGIDVESLCWRILETSLPAQAHA"
     gene            complement(4412..5845)
                     /gene="murC"
                     /locus_tag="PK144_13825"
     CDS             complement(4412..5845)
                     /gene="murC"
                     /locus_tag="PK144_13825"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNF66852.1"
                     /translation="MSSYPIGPHAMRRIRRIHFVGIGGAGMSGIAEVLLNLGYAVSGS
                     DLARGPATQRLAELGADIFIGHDAGHVARRDCIVVSSAVAPDNPELVAAREARIPVVP
                     RAEMLAELMRFRYGIAVAGTHGKTTTTSLIASLLAEGGLDPTFVIGGRLNSAGANARL
                     GAGRYLVAEADESDASFLYLQPMISVVTNIDADHLSTYGGDFNRLRDTFVEFLHHLPF
                     YGLALLCADDPEVRALLPRIARPVLTYGMTEDCDVRATDIVQRGMQTHFTAHLSGGAV
                     ALKVTLNLPGRHSVLNALAAIAVAHELGVSVDAIREALTNFQGIGRRFHSHGHLPLAG
                     GGSVELMDDYGHHPRELAAVLDAIRGGWPERRLVLAFQPHRYTRTRDLFDDFAQVLSG
                     VGVLLLTDVYAAGEKPIPGADGRALARAIRARGRVDPIFVERAADLPAALPAVLRDGD
                     LVLLMGAGDIGAVATRLGREGWPPAEA"
     gene            complement(5842..>6189)
                     /locus_tag="PK144_13830"
     CDS             complement(5842..>6189)
                     /locus_tag="PK144_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF66853.1"
                     /translation="AGVASLLVPFPFAVDDHQSANGRFLERAGAARVLVQATLTPESL
                     AAELAALLPDRERLLAMALAARALARPDAARTVAELGLALAHPAPAPTHPADPANPAD
                     PANPTNSAQDPRT"
BASE COUNT          891 a         2221 c         2196 g          881 t
ORIGIN      
        1 cgtgcgatcg gacgatcggc cggggccgcg ccggcgttgc ggctcgccat cgagcgcacc
       61 gccggctgcg cggccggctg caccgccgcc ggcaccgagg agatgccggt cgcgacgatg
      121 gtcacgcgca gctcgctggt catctccgga tcgatgacgg tgccgatgac gacggtcgcc
      181 tccggcgcgg ccagtgcgtg gacgatctcg ccgacctcgt ggaactcgcc catggtcagg
      241 tcggcgccgg ccgtgacgtt gaccagcagg ccgcgggcac cggccagatc gacgctttcg
      301 agcagcggac tcgcgatcgc ggcctcggcg gcctcgcggg cgcggccgtt gccgctggcg
      361 cgccccgtgc ccatcatcgc cacgcccatc tccgacatca cggtgcgcac gtcggcaaag
      421 tcgacgttga tcaggcccgg gcgcgtgatc agttcggcga tgccctgcac ggcgccggcc
      481 agcacgtcgt tggccgcctt gaaggcatcg agcagcgtcg cgttcttgcc gagcacggcg
      541 agcagcttct cgttcgggat cgtgatcagc gagtcgacct cgtcggcgag ttccttgatg
      601 ccggcgaccg cgacatccat gcgcttgcgg ccctcgaacg ggaagggctt ggtgaccacg
      661 gccacggtca ggatgcccat ctcgcgcgcg acctgcgcga ccaccggcgc gccgccggtg
      721 ccggtgccgc cgcccatgcc ggcggtgatg aacaccatgt cggcgccttc gagcgcctcg
      781 cgcacgcgct cgcggtcttc cattgccgcc ttcttgccga cctcgggatt ggcgccggcg
      841 ccgaggccct tggtgatgcc gctgccgagc tggatcgcat gcggcaccga gtaggtgcgc
      901 agcgcctgcg cgtcggtgtt cgcgcagtag aagtcgacac cctcgatcgt gtggctggtg
      961 accatgtgct gcacggcatt gccgccgccg ccaccgatgc cgatgacctt gatgatcgct
     1021 tcctggtgcg gtgtctcgat gatttcgaac atgtcttcgt ctccccgatg ggcctgaccg
     1081 ccccgtcagg ccggttgcgt ccggtcaccc ggtccgcgcc agccggcgac gcagcagcct
     1141 ctgcgccacc gatgcaaaaa tcactgaaaa aacagaaaca aaaacagaaa caaatcagaa
     1201 attgccctgg aaccacgcct tcatgcgcgc aaacagcccc tggcggttct cgcgcagcgg
     1261 gccggtgcgc accggcccgc gtgcctcggc accgaccagc agcaggccga ccgccgtcga
     1321 gcaggccgga ttggccagca cctcctgcag accggtgacc gccagcgagg tgcccgcgcg
     1381 caccggcacg tggaagatct cctcggccag ctcgaccgca ccgcggatgc gcgagctgcc
     1441 gccggtcagc acgatgccgg cggcgagcat gtcctcgaag ccgctgcggc gcagctcggc
     1501 ctgcaccagc ccgaacagct cctcgtagcg ggcctcgacc acctcggcga gcgtctgcat
     1561 cgacagccgc cgcggtgcgc gctcgccgac gctcggcacg tcgatctcct gatcggccgg
     1621 cgccagcgcc gtcaggcagg tcgcgtgctg gatcttcagg ctctcggcga actgcgtcgg
     1681 cgtgcggaag gccacggcga tgtcgttggt gacctggtca ccggcaatcg ggatcaccgc
     1741 cgagtgctgg atcgcgccgt tgacgaacac cgcgagatcg gtcgtgccgc cgccgatgtc
     1801 gaccaggcag accccgagtt cgcgctcgtc ctcggtcagc accgcacgcg ccgaggcgag
     1861 cggctgcagc acgatgtcgt cgacctccag cgagcagcgc tcgacgcact tgacgatgtt
     1921 ctgcgcggcg ctgaccgcgc cggtgaccat gtgcacgcgc gcctccaggc gcacgccgga
     1981 catgccgatc ggctcgcgga tgccttcctg gtgatcgatc aggaactcct gcgcgaggat
     2041 gtgcaggacc ttctgatcgg tcgggatcgc gacggcgccg gcggcctcga tgacgcggtc
     2101 gatgtcctcc tgcgtcactt ccttgtcgcg gatcggcacg atgccgtgcg agttcaggct
     2161 gcggatatgg ctgccggcga tgccggtgtg cacggagtgg atctggcagc cggcggtgcg
     2221 ctcggcttcc tcgatcgcgt gctggatcga ctgcacggtc gactcgatgt tgacgacgac
     2281 gcccttcttc aggccgcgcg acggatgcat gccgacgccg atgacctcca tcgagccatc
     2341 cgccgcggcc tcaccgacca gcgccacgac cttggacgtg ccgatgtcga ggccgaccac
     2401 cattttcttt tcgttcttgc gcgacataaa aggatgcgtt cccggtttcc ggcgatcagc
     2461 cggcttcgtg cccggtgaag acccgatcct cgctgcgcgc cgtggccggc gcggtggcga
     2521 tcggggctcc ccgggttctg ccatgggtgc cgggctccgc ggacggcgcc ttccagcgca
     2581 ccgcgaaacc gttcaggtac cggagatcca ccccggcgat gcgttcggcc tgggcagcca
     2641 gcacgcgcgg ccagacctcg atgaagcggg cgaggcgctc ggcataggcc tcgcggccga
     2701 gccgcagctc cagcccggtg tcgagggtca gcgtccaggc gccgcgcgca tcctgctgca
     2761 cgcgcacgac cttgagcccc agcggtgcca gcgcacggcc ggtgtcgccg tagacgcgca
     2821 tcagcagcgc ctcgtgtccg tcgggcgcgt cgaagttcgg ccactcgtgc ggctcgcgga
     2881 tcttctgcgg ccggaagcgc gcggccccgg tgtcgatcat ctcgtccctg ccccagtgcg
     2941 cgaacggcac gcgctcgacg aagcgcacct cgacggtgtc cggccagcgg cggctgaccg
     3001 cgaccgactg cacccagggg ttgtcggcga aggccgcgtg caggctgccc atgtcgaggc
     3061 gaaagaagtt gcggccgagg taggggcgca cggcggcctc ggcatcctcg gggccgaggt
     3121 tgcgcaggtc gccgtcgacg tggacctggc gcagcggaaa ttcgccctcg cgcagcatcc
     3181 agtcgacgcc gcgccagccg ccccaggcga tgccgccgag cgcgagcagc gtgccgcaga
     3241 agcgcagcgt gcggccgatc cacggcgaca gcggggccgc cccgtcgccg tcgccgcgca
     3301 gcacgcgcgc ggtcgctccg cgccggcgcc ggcgtcgcag cagcgcactc atgcgtgcgc
     3361 ctgcgccggg agcgaggttt cgaggatgcg ccagcacagc gactcgacat cgatgccggc
     3421 cgcgcgcgca gccatcggca ccagactgtg gtcggtcatg ccgggcacgg tgttgacttc
     3481 gagcagccac ggccggtcct cggcatcgag caccagatcg acgcggcccc agccgctcgc
     3541 cccgaccgcc gcgtaggcac gccggcagag ttcgcgcagc gactgttcca gcgcctccgg
     3601 caggccggcc gggcagtgat agacggtgtc gtcgcggttg tacttggctt cgtagtcgta
     3661 gaactcgacc gcggcctgca tgcggatcag cggcagcgcc gcatcgccga gcacggcgcc
     3721 ggtcagctcg cgaccgacga tccacggctc gatcagcacc ggcgagcggc aggccgccgc
     3781 cagccggaag gcttcggcga gctgtccggc gtgctcgacg cggctgacgc cgatgctgga
     3841 gccctcgcgc gtcggcttga ccgcgagcgg caggccgagc gtctcgatga cgccttcgag
     3901 ctcgcgctcg caggtcacga gccggtgcgc gagcaccggc aggccctcgg cgagccagat
     3961 ctgcttggtg cgcagcttgt ccatgccgat cgccgaggcc tgcacgccgc tgccggtgta
     4021 ggggatgccg agcagctcca gcgcgccctg gatcacgccg tcctcgccgc cgcggccgtg
     4081 cagcacgatg aaggcgcggt cataggcgcc ggcacgcagc acgtcggtca cgtcgcggcc
     4141 ggcatcgatg ccgtgtgcat cgacgccgcg ccggcgcagc gcgccgagca cggtggcgcc
     4201 gctgcgcagc gatacctcgc gttcggccga ccagccgcca tagagcacgg ccaccttgcc
     4261 gaaggcggcg ggatcggtgc agtgcgggct cgggtcggac atcggcttcg tcgtcatctt
     4321 cggttccggg tgcagtgtgg tgcaccgtcg tcattcgggc gctgcgtcgc agcaaaaaaa
     4381 ccggggattc tcgcaaaaag gccgggccgg gtcacgcttc ggccggcggc cagccctcgc
     4441 gaccgagccg ggttgccacc gcgccgatgt cgccggcgcc catcagcagc accagatcgc
     4501 cgtcgcgcag caccgccggc agcgccgccg gcagatcggc ggcacgctcg acgaagatcg
     4561 gatcgacgcg cccgcgcgcc cggatcgccc gcgccagcgc ccggccgtcg gcgccgggaa
     4621 tcggcttctc gccggcggcg tagacatcgg tcagcagcag caccccgacg ccggagagca
     4681 cctgcgcgaa gtcatcgaac aggtcgcgcg tgcgcgtgta gcggtgcggc tggaaggcca
     4741 gcaccagccg ccgctccggc cagccgccgc ggatcgcatc gagcaccgcc gccagctcgc
     4801 gcgggtgatg gccgtagtcg tccatcagtt cgaccgagcc gccgccggcg agcggcaggt
     4861 ggccgtggct gtgaaaacgc cggccgatgc cctggaaatt ggtcagcgcc tcgcggatcg
     4921 catcgaccga cacgccaagc tcgtgcgcga ctgcgatcgc ggcgagcgcg ttcagcacgc
     4981 tgtgccgccc cggcaggttc agcgtcacct tcagcgccac ggcaccgccc gacaggtgcg
     5041 cggtgaagtg cgtctgcatg ccgcgctgca cgatgtcggt ggcgcgcacg tcgcagtcct
     5101 cggtcatgcc gtaggtcagc accggccggg cgatgcgcgg cagcagcgcg cgcacctccg
     5161 gatcatcggc gcacagcagc gccagcccgt agaacggcag gtgatgcagg aactcgacga
     5221 aggtgtcgcg cagccggttg aagtcaccgc cgtaggtcga caggtgatcg gcgtcgatgt
     5281 tggtgaccac cgagatcatc ggctgcaggt acaggaacga ggcgtcggac tcgtccgcct
     5341 cggccaccag atagcggccg gcaccgagcc gcgcgttggc cccggcgctg ttcaggcgcc
     5401 cgccgatcac gaaggtcggg tccagcccgc cctcggcgag caggctggcg atcaggctgg
     5461 tggtcgtggt cttgccgtgc gtgccggcga ccgcgatgcc gtaacggaag cgcatcagct
     5521 cggcgagcat ctcggcgcgc ggcacgaccg ggatgcgtgc ctcgcgcgcc gccaccagct
     5581 ccggattgtc gggcgcgacg gcgctggaga cgacgatgca gtcgcgccgc gcgacgtgtc
     5641 cggcgtcgtg accgatgaag atgtcggcgc cgagttcggc gaggcgctgc gtggccgggc
     5701 cgcgggccag atccgagccc gagaccgcgt agccgaggtt cagcagcacc tcggcgatgc
     5761 cgctcatgcc ggcgccgccg atgccgacga aatggatgcg acggatgcgg cgcatcgcgt
     5821 gcgggccgat cgggtaactg ctcatgtccg ggggtcctgc gcggaattcg tgggatttgc
     5881 cggatcggcc ggatttgccg gatcggccgg gtgcgtcgga gccggtgccg gatgcgcgag
     5941 cgccagcccc agctcggcaa ccgtgcgcgc cgcgtcgggc cgggccagcg cgcgcgcggc
     6001 gagcgccatc gccagcagcc gctcgcgatc gggcagcagc gcggcgagtt cggcggcgag
     6061 ggattcgggc gtcagcgtcg cctgcacgag cacgcgcgcg gccccggcgc gctccaggaa
     6121 gcggccgttg gcgctctggt ggtcgtcgac cgcgaacggg aacggcacca gcaggctggc
     6181 gacgccggc
//
LOCUS       DAOQIE010000242         6163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_6963_length_6163_cov_7.074656, whole genome shotgun sequence.
ACCESSION   DAOQIE010000242 DAOQIE010000000
VERSION     DAOQIE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_6163_cov_7.074656"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1938)
                     /locus_tag="PK144_13835"
     CDS             complement(166..1938)
                     /locus_tag="PK144_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016712752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF66854.1"
                     /translation="MHGIDFIQDLATIMAIAAVVTLLFNRLHQPVVLGYIAAGVLIGP
                     HTPPFALIHDADTIAALSELGVVFLLFTLGLEFSLRKLARVGATALIAALAEIVLMVW
                     LGYQIGRAFGWLTMDSLFLGAMLAISSTTIIVKALDELGMKRERFAQLVFGVLIVEDI
                     LAIGMIALLSGVAAGGALDAGTVLVTLGELSLFMTVSLLVGLLTVPRLLAHVARRGSD
                     EMLLITVLGLCFGFCLLVVKLDYSIALGAFMIGAIMAEARDLALIERLVAPLRDMFSA
                     IFFVSIGLMIDPSVLIEYAWPIAVITVAVVLGKLVSCGLGAWVAGQDGRTAMRVGMGL
                     SQIGEFSFIIAALGVSLKVTSGFLYPIAVAVSAITTLLTPYLIRWADPLSAWLAGRLP
                     APVARVGGLYTDWLHGLRPRGERAELAALVNSILFKVMLNLAVVVALFLGAAAWAPEL
                     GAWLLPWISSGEAHKALIWGGTLLVALPFLIATWRKLKALSLLLAELGCRGRQAGSYS
                     ARLRGLLAEAIPVIALAMILLLIAALSGSLLPGAGVLAVTLAVVAVIAALGWRWFVRV
                     HARLQIELTETLAHQGGEPAAHGH"
     gene            complement(2107..2409)
                     /locus_tag="PK144_13840"
     CDS             complement(2107..2409)
                     /locus_tag="PK144_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66855.1"
                     /translation="MLICLCGFVADAARQVQMLPAREPALLRVLFPVTGGKSREAGLV
                     VVRAPAPCKGPAPLPAGVTSLPPLPGYAALPAPCPAAAESPGRSGWLRPAVRAPPV"
     gene            complement(2669..3964)
                     /gene="glgC"
                     /locus_tag="PK144_13845"
     CDS             complement(2669..3964)
                     /gene="glgC"
                     /locus_tag="PK144_13845"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNF66856.1"
                     /translation="MDPLNNPRYISRLTRNTLALILAGGSGGRLQELVQWRAKPGVFF
                     GGKFRIIDFPISNCMNSGIRRVCVLTQYKSYSLIRHLINAWGSLKGELGEFVEVLPAS
                     QRYSKQWYAGTADAVYQNLDIIRMHRPSHVLVLAGDHIYKMDYGPMLAYHVEKGADMT
                     VACIETPVAEATAFGVMSVDEHARVTRFTEKPAHPESIPGQPGVALASMGNYVFNTDY
                     LFEQLIRDADDPESAHDFSINVIPRMIRDGHSVYAFPFRDPVTGHQPYWRDVGTVDAF
                     WTANMELLETHPELDIYDETWPILTHQVQLPPAKFLFDEPGRTGRAIRSMVSGGCVIS
                     GSEVLHSLLFSKVYVHSWAKVEDSVILPAVDIGRHARIRRAVIDRGCRVPEGMTIGYD
                     PDEDRRRFTVTPKGVVLVTPEMLGQYIHYVREPKRETGG"
     gene            4071..4268
                     /locus_tag="PK144_13850"
     CDS             4071..4268
                     /locus_tag="PK144_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF66857.1"
                     /translation="MAYHEPVIPRDALYSIDTAARTLGVQRQHIERWLASGILHTWDP
                     SGAWPLRGQDLIDFLRRYGQS"
     gene            4280..5227
                     /locus_tag="PK144_13855"
     CDS             4280..5227
                     /locus_tag="PK144_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNF66858.1"
                     /translation="MAGFRPAFGLGGAHRQTLAAALLPAPRPPLARERFELPDGDFVD
                     LDWHRDARGPAVLVLHGLEGDSRSAYARRLLAALARCGRRAGVLHARGRSGEPNRLAR
                     SYTAGDTDDLACVLARLQQRDGRAPDVVGYSLGANRLLKHLGEAAGRAGIRRAVAVSP
                     PFELGRAADRLDRGLSRLYQWHLLRSLRAGYRVKLAQHPQALPLGADDLDALKTFRSF
                     DDRVTAPLHGYAGVEDYYGRCSCRPFLKDIAVPTLIVHALDDPFLYADAIPTPAELAP
                     AVRLELAAHGGHVGFLQGLSWPAQDWLARRIATFLDASD"
     gene            5319..5780
                     /locus_tag="PK144_13860"
     CDS             5319..5780
                     /locus_tag="PK144_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF66859.1"
                     /translation="MPRRPLPLLFAALTLVLAACNSGNRDQNDNPPPVPDYQQTHPTA
                     DARRLQHRGEASFYANHFEGKKMADGTRYDGDSNVAASKRLPLGTKARVKNLETGKTA
                     VVEIRDRGPYVKGRVIDLTPATARSIGIDREDGVAPVEVTPISLPKGQGRG"
     gene            complement(5788..>6163)
                     /locus_tag="PK144_13865"
     CDS             complement(5788..>6163)
                     /locus_tag="PK144_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66860.1"
                     /translation="SARLWDAASGRELRRFEGHGRPVWSVAFSPSGGQVLTGGDDGSA
                     RLWDAETGACLLRLYGFDGNGFDEPGWLSLDARDRYRGNAAGIARLSYVDSDDTSLMP
                     THWHPEDLPYMEAPDSEAPGGG"
BASE COUNT          996 a         2233 c         2108 g          826 t
ORIGIN      
        1 cctcccccgc gaggggggag gggcttgttc atcaacgtct tgccgtcgtt ctgattcgtc
       61 ctcgctgtcc ggacaagttc gggaaccgat gctgcaagtt gtgtagatac ctatgcgctg
      121 cacgaacggc gcggggcgcg aggatccggg gggacgggcg gccgctcagt gcccgtgcgc
      181 agccggctcg ccgccctggt gcgccagcgt ttccgtcagc tcgatctgca ggcgcgcgtg
      241 cacgcgcacg aaccagcgcc agccgagcgc ggcgatcacg gccaccaccg cgagggtgac
      301 ggccagcacg ccggcaccgg gcagcaggct gccgctcagc gctgcgatca gcagcaggat
      361 catcgccagc gcgatcaccg gaatcgcctc ggcgagcagc ccgcgcaggc gcgcgctgta
      421 gctgccggcc tgacggccgc ggcagccgag ttcggcgagc agcaggctca gcgccttgag
      481 cttgcgccag gtcgcgatca ggaagggcag cgccaccagc agcgtcccgc cccagatcag
      541 cgccttgtgc gcctcaccgg agctgatcca cggcagcagc cacgccccga gttccggcgc
      601 ccaggccgcg gcgccgagga acagcgcgac gaccacggcc agattcagca tcaccttgaa
      661 caggatgctg ttgaccaggg ccgccagctc ggcgcgctcg ccgcgcgggc gcagcccgtg
      721 cagccagtcg gtgtagaggc cgccgacgcg cgccaccggc gccggcaggc gcccggcgag
      781 ccaggccgac agcggatcgg cccagcggat caggtaaggc gtcagcagcg tcgtgatcgc
      841 cgacaccgcg accgcgatcg ggtacaggaa gccgctggtg accttcagcg acacgccgag
      901 cgcggcgatg atgaacgaga actcgccgat ctgggacagc cccatgccga cgcgcatcgc
      961 cgtgcgcccg tcctgcccgg ccacccaggc gccgagcccg cagctcacca gcttgccgag
     1021 cacgaccgcg accgtgatca ccgcgatcgg ccatgcgtat tcgatcagca ccgacgggtc
     1081 gatcatcagc ccgatcgaca cgaagaagat cgcgctgaac atgtcgcgca gcggcgcgac
     1141 cagccgctcg atcagcgcca gatcacgcgc ctcggccatg atcgcgccga tcatgaaggc
     1201 gccgagcgcg atgctgtagt cgagcttcac gaccagcagg cagaagccga agcagagccc
     1261 gagcaccgtg atcagcagca tctcgtcgct gccgcggcgc gcgacgtgcg cgagcagccg
     1321 cggcacggtc agcagcccca ccagcagcga cacggtcatg aacagcgaca gctcgccgag
     1381 cgtgaccagc acggtcccgg catcgagcgc accgccggcg gccacgcccg agagcagcgc
     1441 gatcatgccg atcgcgagga tgtcctcgac gatcagcacg ccgaacacca gctgcgcgaa
     1501 gcgctcgcgc ttcatgccga gctcatcgag cgccttgacg atgatcgtcg tcgacgagat
     1561 cgccagcatc gcgccgagga acagcgaatc catcgtcagc cagccgaagg cgcggccgat
     1621 ctggtagccg agccagacca tcagcacgat ctcggcgagt gccgcgatca gcgcggtcgc
     1681 gccgacccgc gcgagcttgc gcaggctgaa ctccagcccg agcgtgaaca gcaggaacac
     1741 cacgccgagt tccgacagcg cggcgatggt gtcggcgtcg tggatcagcg cgaacggcgg
     1801 cgtatgcggg ccgatcagca caccggcggc gatgtagccg agcaccacgg gctggtgcag
     1861 gcggttgaac agcagcgtga cgacggcggc gatcgccatg atcgtcgcca ggtcctggat
     1921 gaaatcgata ccgtgcatgg tcggggtctc cgcgcaccgg ggcacaccgc cggcccgggg
     1981 aaaggaaccg cgcggcgcgt cagacagttc tcgcacccgc gcagcaggcg ggcgcagcgg
     2041 cgaggggtgc cgcgcggccg cggctgcgtc cggatgcgga gcaggcaggc gcacggcacg
     2101 gccggttcag accggcggcg cccgcacggc cggccgcagc cagccggaac gcccgggaga
     2161 ctccgcggct gccgggcacg gagccggcag ggctgcgtac ccgggcagcg gcggcagcga
     2221 agtgacgccg gccggcagcg gcgccggtcc cttgcacggc gccggcgccc gcaccacgac
     2281 cagccccgcc tcgcgcgact tgccgccggt aaccgggaac agcacgcgca gcagcgccgg
     2341 ttcgcgtgcc ggcagcatct gcacctgtcg cgctgcatcg gcaacgaagc cgcacaggca
     2401 gatcagcacc agcagccacg ccagccccgc gccggccggc agctgtcgtc cgggcgcacc
     2461 cggacgcgcc gcccgggcag gcggcgttgt tacaggtggg atgagccggg tgcaggacat
     2521 gggccggagt cttgcattcc ctgcgccgcc gggtcgagtc cggatgtccg gcgcatggca
     2581 acgggtggaa gcccgggctg acggcgccgc tgtgcaacac gcccttgccc gcagcccggc
     2641 gggcggcttg gacgccccgt ggctgccctc agcctccggt ctcgcgcttc ggctcgcgca
     2701 cgtagtggat gtactggccg agcatctccg gcgtgaccag caccacgccc ttcggcgtca
     2761 ccgtgaagcg gcggcggtcc tcgtccgggt cgtagccgat cgtcatgccc tcgggcacgc
     2821 ggcagccgcg gtcgatgacg gcgcggcgga tgcgggcgtg gcggccgatg tcgacggccg
     2881 gcaggatcac cgagtcctcg accttcgccc acgaatgcac gtagaccttc gagaacagca
     2941 gcgaatgcag cacctcgctg cccgagatca cgcagccgcc cgagaccatc gagcggatcg
     3001 cgcgcccggt gcgccccggc tcgtcgaaca ggaacttcgc cggcggcagc tgcacctgat
     3061 gggtcaggat cggccaggtc tcgtcgtaga tgtcgagttc cggatgggtt tcgagcagct
     3121 ccatgttcgc ggtccagaac gcatcgacgg tgccgacatc gcgccagtac ggctggtggc
     3181 cggtgaccgg gtcgcggaac ggaaacgcgt agacgctgtg accgtcgcgg atcatgcgcg
     3241 ggatgacgtt gatgctgaag tcgtgcgcgg actccgggtc atcggcgtcg cggatcagct
     3301 gctcgaacag gtaatcggtg ttgaacacgt agttgcccat cgacgccagc gcgacgccgg
     3361 gctggccggg gatgctctcg ggatgagcgg gcttctcggt gaagcgcgtc acgcgcgcgt
     3421 gctcgtcgac cgacatgacg ccgaaggccg tcgcctcggc caccggcgtc tcgatgcagg
     3481 cgacggtcat gtccgcgccc ttctcgacgt ggtacgcgag catcggaccg tagtccatct
     3541 tgtagatgtg atcgccggcg agcacgagca cgtggctcgg gcggtgcatg cggatgatgt
     3601 cgaggttctg gtacacggcg tcggccgtgc cggcgtacca ctgcttcgag tagcgctgcg
     3661 aggccggcag cacctcgacg aactcgccga gctcgccctt cagcgagccc caggcgttga
     3721 tcaggtggcg gatcagcgag tacgacttgt actgcgtcag cacgcagacc cggcggatgc
     3781 cggagttcat gcagttcgag atcgggaagt cgatgatccg gaacttgccg ccgaagaaca
     3841 cgccgggctt ggcgcgccat tgcacgagtt cctgcagccg tccgccgctg ccgcccgcga
     3901 ggatcagcgc gagcgtgttg cgggtcagtc ggctgatgta acgcggattg ttcagcgggt
     3961 ccatggcggg ccggaacctc gtcgcgggcg gtgggcttgg gctggcgggg ccgggcatct
     4021 taccatcacg cccctgcccg cccggcagcc gtccgagtca gtgcccgatc atggcttatc
     4081 acgaacccgt catcccgcgc gatgcgctgt actcgatcga caccgccgcg cgcacgctcg
     4141 gcgtgcagcg ccagcacatc gagcgctggc tcgcgagcgg catcctgcac acctgggacc
     4201 cgagcggcgc ctggccgctg cgcggccagg atctcatcga cttcctgcgt cgctacggcc
     4261 agtcctgagc tggatcaccg tggccggctt ccgtccggcc ttcggtctcg gcggcgcaca
     4321 ccgccagacg ctcgccgccg cgctgctgcc ggcaccccgc ccgcccctcg cccgcgaacg
     4381 cttcgaactg ccggacggcg atttcgtcga tctcgactgg caccgcgacg cgcgcggccc
     4441 ggccgtgctg gtgctgcacg gcctcgaagg cgacagccgt tccgcctacg cccgccggct
     4501 gctcgcggcg ctcgcgcgct gcggacgacg cgccggcgtg ctgcacgcac gcgggcgctc
     4561 cggcgaaccg aaccgcctgg cgcgcagcta cacggccggc gacaccgacg atctcgcctg
     4621 cgtgctcgcg cgcctgcagc agcgcgacgg ccgcgcgccg gacgtggtcg gctattcgct
     4681 cggggccaac cgcctgctca agcacctcgg tgaggccgca ggccgggccg gcatccggcg
     4741 tgcggtcgcc gtgtcgccgc cgttcgaact cggccgcgcc gccgaccggc tcgaccgcgg
     4801 actgtcgcgg ctgtaccagt ggcatctgct gcgcagcctg cgcgccggct atcgcgtgaa
     4861 gctcgcgcag catccgcagg cgctgccgct cggggcggat gatctcgatg cgctgaagac
     4921 cttccgcagc ttcgacgatc gcgtcaccgc gccgctgcac ggctatgccg gggtcgagga
     4981 ctactacggc cgctgcagct gccggccttt cctgaaagac atcgcggtgc cgacgctgat
     5041 cgtgcacgcc ctcgacgatc ccttcctgta cgccgatgca atcccgacgc ccgcggaact
     5101 ggcaccggcc gtgcgcctgg aactggccgc acacggcggt cacgtcggct tcctgcaggg
     5161 tctgtcatgg cctgcgcagg actggctcgc gcggcgcatc gcgacctttc tcgacgcaag
     5221 cgactgacgc gaaacgcgcc tgcggcgaac ctccggcgaa accggcgggt cacaaagtcc
     5281 gtccgtgacc gctgccgacc gcgcaggaga caaccacaat gccccgccgc ccgctgccgc
     5341 tgctgttcgc cgccctgacc ctggtgcttg ccgcctgcaa ctccggcaac cgcgaccaga
     5401 acgacaatcc gccgcccgtc cccgattacc agcagaccca cccgaccgcc gatgcccgac
     5461 gcctgcagca tcgcggcgag gcttcgttct acgccaacca cttcgagggc aagaagatgg
     5521 ccgatggcac gcgctacgac ggcgacagca acgtcgccgc cagcaagcgc ctgccgctcg
     5581 gcaccaaagc cagggtgaag aacctcgaaa ccggcaagac tgccgtcgtc gaaatccgcg
     5641 accgcggccc gtacgtcaaa ggccgcgtca tcgacctgac cccggccacg gcccgcagca
     5701 tcggcattga ccgcgaggac ggcgtggcgc cggtggaggt gacgccgatt tcgttaccga
     5761 agggtcaggg acgcgggtaa gcgaacctca gccgccgcct ggcgcctcgg agtccggcgc
     5821 ttccatataa ggaagatctt cggggtgcca gtgcgtgggc atcaggctgg tgtcatctga
     5881 atcgacgtag ctcaggcgag cgatgccggc ggcgttgccg cggtagcgat cccgggcgtc
     5941 gagtgacagc cagccgggtt cgtcgaagcc gttgccgtca aaaccgtaca ggcgcagcag
     6001 gcaggcaccg gtttcggcat cccacagccg cgcgctgcca tcgtccccgc cagtcagcac
     6061 ctgcccgccc gacggcgaga acgccaccga ccacaccgga cgtccatgtc cctcaaagcg
     6121 gcgcagttca cgcccgctgg ccgcatccca cagccgcgca ctg
//
LOCUS       DAOQIE010000243         6142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7002_length_6142_cov_8.097421, whole genome shotgun sequence.
ACCESSION   DAOQIE010000243 DAOQIE010000000
VERSION     DAOQIE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_6142_cov_8.097421"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /gene="rpsK"
                     /locus_tag="PK144_13870"
     CDS             complement(<1..115)
                     /gene="rpsK"
                     /locus_tag="PK144_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNF66861.1"
                     /translation="MAKPAVKTRKRVKKNVVDGIAHVHASFNNTIITITDRQ"
     gene            complement(163..519)
                     /gene="rpsM"
                     /locus_tag="PK144_13875"
     CDS             complement(163..519)
                     /gene="rpsM"
                     /locus_tag="PK144_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNF66862.1"
                     /translation="MARIAGINIPVNKHAVIALQSIYGIGQTRAKAICAAADVAPETK
                     VKDLVEAQVEALRAEVAKFVVEGDLRREVSMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     RTNARTRKGPRRAVKK"
     gene            complement(612..725)
                     /gene="rpmJ"
                     /locus_tag="PK144_13880"
     CDS             complement(612..725)
                     /gene="rpmJ"
                     /locus_tag="PK144_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNF66863.1"
                     /translation="MKVRASVKKLCRNCKIIRREGVVRVICKDARHKQRQG"
     gene            complement(806..2131)
                     /gene="secY"
                     /locus_tag="PK144_13885"
     CDS             complement(806..2131)
                     /gene="secY"
                     /locus_tag="PK144_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNF66864.1"
                     /translation="MSAPLPSIGKMTELRARLLFLLGALIVFRIGTHIPVPGIDPHAM
                     AQLFQQQRGTILDMFNMFSGGALQRLSIFALGIMPYISASIIVQLMTVIVPTLERLKK
                     EGNAGRRKITQYTRYLTVVLATFQSVGVSIALQGQSAGGLAVVPHPGIGFVAISAITL
                     VSGTLFLMWLGEQVTERGIGNGISMLIFAGIVAGLPAALGGTLELARTGELNALLVIA
                     LLVVALVVTALVIFVERGQRRITVNYAKRQEGRKVYAAQSSHLPLKLNMAGVIPPIFA
                     SSLILFPATIGSWFGNTPGMGWLRDLSSTLAPGHPLYVALYATLIVFFCFFYTALVFN
                     SRETADQLKKSGAFIPGIRPGAQTATYIDRVLTRLTLAGAIYITGVCLLPEFLILYWK
                     VPFYFGGTSLLIIVVVVMDFMAQVQAHLMSHQYESLMKKANFGAGGALR"
     gene            complement(2137..2571)
                     /gene="rplO"
                     /locus_tag="PK144_13890"
     CDS             complement(2137..2571)
                     /gene="rplO"
                     /locus_tag="PK144_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNF66865.1"
                     /translation="MRLNTLKPGEGARQEGKRVGRGIGSGLGKTCGRGHKGQHSRAGG
                     FHKVGFEGGQMPLQRRLPKVGFRSLTARDTAEVRLHELNGIDAEVIDIHALKAAGIVA
                     QPALFAKVVLSGELSRAVTLKGVAVTRGARAAIEAAGGKVEE"
     gene            complement(2574..2759)
                     /gene="rpmD"
                     /locus_tag="PK144_13895"
     CDS             complement(2574..2759)
                     /gene="rpmD"
                     /locus_tag="PK144_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNF66866.1"
                     /translation="MSASKVKVTLVKSTSGRLAAHKACVRGLGLRRMHHSTVVIDTPE
                     NRGMINKVSYLLKVEEA"
     gene            complement(2763..3272)
                     /gene="rpsE"
                     /locus_tag="PK144_13900"
     CDS             complement(2763..3272)
                     /gene="rpsE"
                     /locus_tag="PK144_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNF66867.1"
                     /translation="MATNVESSQNTDGLNEKLIAVNRVAKVVKGGRQFGFTALTVVGD
                     GQGRIGFGYGKAREVPVAIQKAMEKARKNMSTIALAGTTLQHPLNGEHGAARVFMQPA
                     SEGTGIIAGGAMRAVFEVLGVKDVLAKCVGSRNPINVVRATINGLKGMASPDYIAAKR
                     GKKVEEILG"
     gene            complement(3284..3637)
                     /gene="rplR"
                     /locus_tag="PK144_13905"
     CDS             complement(3284..3637)
                     /gene="rplR"
                     /locus_tag="PK144_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNF66868.1"
                     /translation="MDKKAARIRRGLRARMKIRELGAVRLVVHRTPKHIYAQIIAPNG
                     SQTLVAASTLEKGVIEGLGFTGNIEAARAVGKAIAEKAKAAGISEVAFDRSGFKYHGR
                     VQALADAAREHGLQF"
     gene            complement(3649..4182)
                     /gene="rplF"
                     /locus_tag="PK144_13910"
     CDS             complement(3649..4182)
                     /gene="rplF"
                     /locus_tag="PK144_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNF66869.1"
                     /translation="MSRVAKKPVALPAGVSVAVDGRKVSVKGAKGALDFELHPTVAIV
                     HENNELKLVPQSEAAEHRAMAGTMRALVANMVTGVSKGFERKLQLVGVGYRAQAQGAA
                     LNLTLGFSHPVVFDVPQGITVETPTQTEILVKGVDKQKVGQVAAKIRAFRPPEPYKGK
                     GVRYADETIVLKEAKKK"
     gene            complement(4203..4598)
                     /gene="rpsH"
                     /locus_tag="PK144_13915"
     CDS             complement(4203..4598)
                     /gene="rpsH"
                     /locus_tag="PK144_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNF66870.1"
                     /translation="MSMTDPIADMLTRIRNGQSAGKQQVSLPASKLKAAVAKVLSDEG
                     YIGECAITDVDGKPQLTIALKYYQGRPVIETIQRVSRPGLRIFRGRDELPKVMGGLGI
                     AVVSTSHGIMTDRAARAAGYGGEVICVVA"
     gene            complement(4714..5019)
                     /gene="rpsN"
                     /locus_tag="PK144_13920"
     CDS             complement(4714..5019)
                     /gene="rpsN"
                     /locus_tag="PK144_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNF66871.1"
                     /translation="MAKTSMINREVKRARLVQKYAAKRAELKELIRSPATADDARREA
                     QAKLQALPRDASPCRQRNRCRLTGRPHGFYRKFGLSRNKLREAAMRGDIPGLRKASW"
     gene            complement(5034..5573)
                     /gene="rplE"
                     /locus_tag="PK144_13925"
     CDS             complement(5034..5573)
                     /gene="rplE"
                     /locus_tag="PK144_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNF66872.1"
                     /translation="MARLQDHYRDVVVKKLTERFGYQNVMQVPRITKITLNMGVGEAV
                     GDKKVIEHAVSDMTAIAGQKPVVTLARKSVAGFKIREGWPIGCKVTLRRDRMYEFLDR
                     LVAIALPRIRDFRGINGRCFDGRGNYSFGVKEQLMFPEIDYDRVDAVRGMDICITTTA
                     KTDEEGRALLEAFSFPFRN"
     gene            complement(5584..5901)
                     /gene="rplX"
                     /locus_tag="PK144_13930"
     CDS             complement(5584..5901)
                     /gene="rplX"
                     /locus_tag="PK144_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNF66873.1"
                     /translation="MRKIKKGDEVIVTTGKDKGRTGKIVRVLDTERVVVEGVNLIKKH
                     QKPNPAKGVAGGIVEREASIHVSNVALFNPVTKKADRVGIRTLEDGRKVRFYKSTNEV
                     VDA"
     gene            complement(5922..>6142)
                     /locus_tag="PK144_13935"
     CDS             complement(5922..>6142)
                     /locus_tag="PK144_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HNF66874.1"
                     /translation="KVKKGDVYNAVVVRTRKGVRRADGSLIRFDGNAAVLLNNKLEPI
                     GTRIFGPVTRELRSERFMKIISLAPEVL"
BASE COUNT          997 a         2020 c         1998 g         1127 t
ORIGIN      
        1 cctggcggtc ggtgatggtg atgatcgtgt tgttgaacga cgcgtggacg tgcgcaatgc
       61 cgtcgacgac gttcttcttg acgcgcttgc gcgtcttcac agcgggcttg gccatgaagg
      121 gtacctgtca gcctgatacg gtgggtcgct ggtcgcggcg gcttacttct tgacggcgcg
      181 gcgcgggccc ttgcgggtgc gggcgttggt gcgcgtgcgc tgtccgcgca gcggcaggcc
      241 gcgacggtga cgcaggccgc gatagcagcc gaggtccatg agacgcttga tgttcatgga
      301 cacttcgcgc cgcagatcgc cttcgacgac gaacttcgcg acctcggcgc gcagcgcctc
      361 gacctgtgcc tcgaccagat ccttgacctt ggtctccggc gccacatcgg cagccgcaca
      421 gatcgccttc gcgcgcgtct ggccgatgcc gtagatggac tgcaacgcga tcaccgcgtg
      481 cttgttcacc ggaatgttga tgcctgcaat acgggccatg ccgaaactcc caaaatttcg
      541 tacgcgcaaa ggctggcgcg tgaaacgccg aagaataaac cgtttcccga attcgggcaa
      601 cggcgtagcg atcagccctg acgctgcttg tggcgggcgt ccttgcagat cacgcgcacc
      661 acgccttcac gacggatgat cttgcagttg cggcagagct tcttgacgga ggcacgaact
      721 ttcatctgga ttctcccggc ggccggcatc atctgtgctg cggtcgccaa atcattggtt
      781 gactggcctg aagatgccgg cgcggtcagc gcagggcgcc accggcgccg aaattggcct
      841 tcttcatcag actttcgtac tggtgcgaca tcaggtgggc ctggacctgg gccatgaagt
      901 ccatgaccac gaccacgatg atcagcagcg aggtgccacc gaagtagaac ggcaccttcc
      961 agtacaggat caggaactcg ggcagcaggc acacgccggt gatgtagatc gctccggcca
     1021 gcgtcagccg cgtcagcacc cggtcgatgt aggtggccgt ctgcgccccc ggacggatgc
     1081 ccggaatgaa cgcgcccgac ttcttcagct ggtctgcggt ttcccgcgag ttgaacacca
     1141 gcgcggtgta gaagaagcag aagaacacga tcagcgtcgc atacagcgcc acgtacagcg
     1201 gatgacccgg ggccagcgtc gaactgagat cacgcagcca gcccatgccc ggcgtattgc
     1261 cgaaccacga gccgatcgtg gccgggaaca ggatcaggct cgacgcgaag atcggcggga
     1321 tcacgccggc catgttgagc ttcagcggca gatggctcga ctgcgccgca tagaccttgc
     1381 ggccttcctg gcgcttggcg tagttgacag tgatgcgccg ctggccgcgc tcgacgaaga
     1441 tcaccagcgc agtcaccacc agcgccacga ccagcagcgc gatcaccagc agcgcgttca
     1501 gctcgccggt gcgggcgagt tcgagcgtgc cgccgagggc tgccggcagg ccggcgacga
     1561 tgccggcgaa gatcagcatc gagatgccgt tgccgatgcc gcgctcggtg acctgttcgc
     1621 ccagccacat caggaacagc gtgcccgaca ccagcgtgat cgccgagatc gccacgaagc
     1681 cgatgcccgg atgcggcacc accgccagac cgccggcgct ctgcccctgc agcgcgatgc
     1741 tgacgccgac cgactggaag gtcgccagca cgaccgtcag gtagcgcgtg tactgcgtga
     1801 tcttgcgccg cccggcattg ccttccttct tcagccgctc cagcgtcggc acgatcaccg
     1861 tcatcagctg cacgatgatg gaagcggaga tgtacggcat gatgccgagc gcaaagatcg
     1921 acagccgctg cagcgcgccg cccgagaaca tgttgaacat gtccaggatc gtgccgcgct
     1981 gctgctggaa cagctgcgcc atcgcgtgcg ggtcgatgcc gggcaccggg atgtgggtac
     2041 cgatgcggaa cacgatcagc gcgccgagca ggaacagcag gcgggcgcgc agttcggtca
     2101 tcttgccgat cgagggcagc ggtgccgaca tgggacttac tcttcgacct tgccgcccgc
     2161 cgcttcgatc gcggcgcgcg cacccctggt gacggccacg cccttgagcg tgacggcacg
     2221 gctcagttca ccggacagca cgaccttcgc gaagagagcc ggctgcgcaa cgatgccggc
     2281 cgccttcagg gcgtggatgt cgatgacttc ggcatcgatg ccgttgagct cgtgcaggcg
     2341 cacctcggcg gtatcacgcg cggtcagcga ccggaagccg accttcggca gccggcgctg
     2401 cagcggcatc tggccgcctt cgaaaccgac cttgtggaag ccaccggcgc gcgagtgctg
     2461 acccttgtgg ccgcggccgc aggtcttgcc cagaccggaa ccgatgccac ggccgacgcg
     2521 cttgccttcc tgccgggcgc cttcgcccgg cttcagcgta ttcaggcgca tgctcaggcc
     2581 tcctcgacct tcagcagata cgacaccttg ttgatcatgc cccggttttc cggggtatcg
     2641 atcacgacgg tgctgtgatg catgcggcgc agtccgagac cgcggacaca agccttgtgt
     2701 gcggccagcc gaccgctggt gctcttgacg agcgtgacct tcaccttgct agcggacatg
     2761 gctcacccca ggatctcttc gactttcttg ccgcgcttgg cggcgatgta gtccggcgag
     2821 gccatgccct tgagcccgtt gatcgtcgcg cgcacgacgt tgatcgggtt gcgggaaccg
     2881 acgcacttgg ccagcacgtc cttgaccccg agcacctcga acacggcgcg catcgcaccg
     2941 ccggcgatga tgccggtacc ttccgaggcc ggctgcatga acacgcgggc cgcgccgtgc
     3001 tcgccgttca gcgggtgctg cagcgtggtg ccggcgagcg cgatcgtgct catgttcttg
     3061 cgcgccttct ccatcgcctt ctggatcgcg accggcactt cacgggcctt gccgtagccg
     3121 aagccgatgc ggccctggcc atcgccgacc acggtcagcg ccgtgaagcc gaactgccgg
     3181 ccgcccttga cgaccttggc gacacggttg acggcgatca gcttctcgtt caggccatcg
     3241 gtattctgcg aagactcgac attcgtagcc atggacccaa cccttagaac tgcagtccgt
     3301 gctcacgcgc cgcatcggcg agcgcctgca cacggccgtg atacttgaaa ccggagcggt
     3361 cgaaggcgac ctcggagatg ccggcggcct tggccttctc ggcgatcgcc ttgcccacgg
     3421 cgcgggcggc ctcgatgttg ccggtgaacc cgagtccctc gatcacgccc ttttcgagcg
     3481 tcgaggcggc aaccagcgtc tggctgccgt tcggggcgat gatctgcgcg tagatgtgct
     3541 tcggcgtacg gtgcacgacc aggcgcacgg cacccagttc gcgaatcttc atccgggcac
     3601 gcaggccccg gcggatgcga gcagctttct tgtccatttc cgggcccctt acttcttctt
     3661 cgcctctttg aggacgatgg tttcgtcggc gtaccgcacg cccttgccct tgtagggctc
     3721 cggcggacgg aaggcgcgga tcttggcggc gacctggccg accttctgct tgtcgacgcc
     3781 cttgacgagg atttcggtct gcgtcggggt ctcgacggtg atgccctgcg gcacgtcgaa
     3841 caccaccgga tgcgagaagc cgagcgtgag gttcagcgcc gcaccctgtg cctgggcgcg
     3901 gtaaccgacg ccgaccagct gcagcttgcg ctcgaagccc ttgctgacgc cggtgaccat
     3961 gttggcgacc agcgcgcgca tcgtgccggc catggcgcgg tgttccgcgg cctcggactg
     4021 cggcacgagc ttgagttcat tgttttcgtg gacgatggcc accgtcgggt gcagttcgaa
     4081 atccagggca cccttggcac ccttgaccga caccttgcga ccgtcgaccg ccacactgac
     4141 gccggcaggc agggccaccg gcttcttggc aacgcgagac atgactcgat ctccttccgg
     4201 gttcaggcga cgacgcagat gacttcgccg ccgtagccgg cagcgcgcgc cgcgcgatcg
     4261 gtcatgatgc cgtgggacgt ggacaccacg gcaatgccga ggccacccat gaccttgggc
     4321 agctcgtcac ggccacggaa gatgcgcagg cccggacggc tcacgcgctg gatggtctcg
     4381 atgaccgggc ggccctggta gtacttcagg gcgatcgtca gctgcggctt gccatcgacg
     4441 tcggtgatgg cgcactcgcc gatgtagcct tcgtcgctca gcaccttggc gacggcggcc
     4501 ttcagcttcg aggcgggcag cgaaacctgc tgcttgccgg cggactggcc gttgcggata
     4561 cgcgtcagca tatccgcgat gggatcggtc atgctcattg ctaaagaact ccagtgggtc
     4621 agtgtcaggg cagccggtga acctgtgcag cccctcgact taagacctgg gttgtgaaaa
     4681 acgtgggttg cgcgaaaacg cgacggacgg aacttaccag ctcgccttgc gcaggcccgg
     4741 gatgtcgccg cgcattgccg cttcacgcag cttgttacgc gacaggccga acttgcggta
     4801 gaagccgtgc gggcggccgg tcaggcggca gcggttgcgc tgacggcagg ggctggcgtc
     4861 acgcggcagc gcctgcagct tcgcctgcgc ctcgcggcgg gcgtcgtcgg cagtcgcggg
     4921 gctgcggatc agctccttga gctcggcgcg cttggcagcg tacttctgca cgaggcgcgc
     4981 acgcttgacc tcgcggttga tcatggacgt cttggccacg ggatcgcgtc ctgtcagttg
     5041 cggaacggaa agctgaaggc ttcgagcagg gcacggccct cctcgtcggt cttcgccgtc
     5101 gtcgtgatgc agatgtccat gccacgcacg gcatcgacgc ggtcgtagtc gatctccggg
     5161 aacatgagct gttccttgac accgaagctg tagttgccac gaccgtcgaa gcagcggccg
     5221 ttgatgccgc ggaagtcacg gatgcgcggc agcgcgatcg cgaccagccg gtcgaggaac
     5281 tcgtacatgc ggtcccggcg cagcgtgacc ttgcagccga tcggccagcc ttcgcggatc
     5341 ttgaaaccgg ccaccgactt gcgcgccagc gtcaccaccg gcttctggcc ggcgatcgcg
     5401 gtcatgtcgg aaacggcgtg ctcgatgacc ttcttgtcgc cgaccgcctc gcccacgccc
     5461 atgttgagcg tgatcttggt gatgcgcggc acttgcatga cgttctggta gccgaagcgc
     5521 tcggtgagct tcttgaccac gacgtcgcga tagtgatcct gcagtcttgc catgtctgtc
     5581 gcctcaggcg tccacgactt cgttggtgga cttgtaaaaa cgcaccttgc ggccgtcttc
     5641 gagcgtgcgg atgccgaccc ggtcggcctt cttggtgacc ggattgaaca gcgccacgtt
     5701 ggagacgtgg atcgaggcct cgcgttcgac gataccgccg gcgacgccct tggccggatt
     5761 cggcttctgg tgcttcttga tgaggttcac gccttcgacg accacgcgct cggtgtcgag
     5821 cacgcgaacg atcttgccgg tgcgaccctt gtccttgccc gtggtgacga tgacttcgtc
     5881 acccttcttg atcttgcgca tcgtgttgct ctccgcgctc ctcagagcac ctcgggtgcc
     5941 agggagatga tcttcatgaa gcgctcgctg cgcagctcgc gggtcacggg cccgaagatg
     6001 cgggtaccga tcggctccag cttgttgttc agcagcaccg cggcgttgcc gtcgaagcgg
     6061 atcagcgagc catcggcccg gcgcacaccc ttgcgggtgc gcacgacgac ggcgttgtag
     6121 acgtcgccct tcttgacctt gc
//
LOCUS       DAOQIE010000244         6134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7022_length_6134_cov_7.428689, whole genome shotgun sequence.
ACCESSION   DAOQIE010000244 DAOQIE010000000
VERSION     DAOQIE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_6134_cov_7.428689"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..972
                     /locus_tag="PK144_13940"
     CDS             538..972
                     /locus_tag="PK144_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66875.1"
                     /translation="MKKIQALKLTLALLAAGMSMLAPVKLVHAQQYYTRYIYANSVCR
                     YPIRMMVYHKDSENNYHPHGWYNFRPYEESRLKAHNVVLRQVVGYDLYFYAETDNRYN
                     VPAMSWARGDAAVNFEGIYYNLTRANLFVNTKGELEFKLSCA"
     gene            complement(1121..2065)
                     /locus_tag="PK144_13945"
     CDS             complement(1121..2065)
                     /locus_tag="PK144_13945"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNF66876.1"
                     /translation="MSVYTPVDRAQLETFLRDYDIGDYVAHRGISEGISNTNYFLTTT
                     RTEVVLTLFELIGFEDVPYYLDLMAWLAARGVPGANPVADRQGHYMKPLAGKPAALVH
                     KLAGGGLHGTPSLAQCREIGRALAQLHLAGEGFAQRRDNDRGPRWCQSAAERVLPLLD
                     RVEAHLLNDELAALTEHPRSRLPQGTIHADLFRDNALFDGDRLCGLIDFYYACTDALA
                     FDVAVTVNDWCTQPDGTLDRERAAALYGAYAALRPFSEAERAEWPRVLRAAALRFWLS
                     RLVDKLFPTPGELTFIKDPDAFKHILVAHRGAPWRLAD"
     gene            complement(2148..2483)
                     /locus_tag="PK144_13950"
     CDS             complement(2148..2483)
                     /locus_tag="PK144_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNF66877.1"
                     /translation="MRSLLLSVLLLAAAGPVALAADEPAPKPPELSPLPESGVDEQPV
                     PDVTITRRAEDTVAEYRINGRLYMIKVTPKKGLPYFLIDSDGDGNLETRHNDLDPGIA
                     IPSWVLLRW"
     gene            complement(2526..3251)
                     /locus_tag="PK144_13955"
     CDS             complement(2526..3251)
                     /locus_tag="PK144_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF66878.1"
                     /translation="MSPENKQSHPGMAPVNDALLTRESWKIFQIMAEFVEGFERLARI
                     RPSVSIFGSARTQPGHPYYVLAEQIGLLLSDAGFSVVTGGGPGIMEAGNKGAYAGRSP
                     SIGLNIQLPHEQLGNPYQNIALSFRHFFSRKVMFVKYASAYVVLPGGFGTLDEMAEIM
                     TLVQTGKTRRIPIILVHSPFWSPLLAWFRDTLVPEGMIDGGDLELLQLVDDPQQVVDV
                     IFSHYESRGFEPSAEEQEILLDL"
     gene            3393..6134
                     /gene="polA"
                     /locus_tag="PK144_13960"
     CDS             3393..6134
                     /gene="polA"
                     /locus_tag="PK144_13960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNF66879.1"
                     /translation="MTVLLVDGSSYLYRAFHALPPLSTARGEPTGAVRGVIAMLRNLV
                     TRLAPSRIAVVFDASGPTFRDAIYPAYKAHRPPMPDELRAQIEPLHALVRALGLPLLC
                     EPGVEADDVIGTLARAATAAGESVVIATGDKDLAQLVDARITLLDTMKRDGQETVLDH
                     DGVIAKFGVPPAGIVDLLALMGDSVDNIPGVPGVGPKTAAKWLAEHGSLAALVAGADG
                     IKGKAGEALRAHLAQLDLARQLATIRCDVPLPFGLEALAPQPPDRATLRTLYERLEFR
                     TWLKELDAAGEPVAGAAADSAPAAEAAPPPAPSPARDYETVLDEAAFAGWLARLEAAD
                     AFAFDTETDALDYMRARIVGVSFAIEPQRAAYVPLAHVYPGAPDQLDRDSVLARLRPL
                     LEDPHKGKIGQHAKFDINVLANHGIALAGLADDTMLESYVLDATATRHDMDSLAALYL
                     DEKTIKYEDVAGRGAKQIGFEQVPIENAGPYAAEDADVTLRLHLALSAKLAEVPALMG
                     VYRDIEMPLVPVLSRMERRGVRIDHAGLRRMSAEFAQRLLELEREAHDLAGQPFNLGS
                     PKQLQSILFERQQLPVKKKTPTGQPSTDEEVLAELAEDGHALPKVILEHRSLSKLRGT
                     YTDRLPELVNPVTGRVHTSYHQAVASTGRLSSSDPNLQNIPVRTDAGRRIRSAFIAEP
                     GCCVVAADYSQIELRIMAHLSGDAALLAAFARGDDIHRATAAEVFGLTLEQVAPEQRR
                     AAKAINFGLIYGMSAFGLARQLGIERGAAQDYVSRYFARYPGVKTFMDETRRSAAARG
                     YVETVSGRRLYLPEINSKNPARRAAAERTAINAPMQGTAADIIKRAMIAVDGWIEAAG
                     VPARMILQVHDELVFEVEEAALEAFCKEVRTHMSAAAELSVPLLVEVGSGRNWDEAH"
BASE COUNT          995 a         2110 c         2058 g          971 t
ORIGIN      
        1 ggctccggcc ggacgatccg tttgcgctcg gagcaggtct ttcggtcatc ggcagtcctg
       61 tccggaggca tgaccggctg atcgagcagc ccgatccctg ccaacggatg gtcaggcact
      121 ggcagaggta cctgtctgcc ctgcctgcag cggcttcggc cggcatcgcc ggtgcccgtg
      181 ccccgtccgc tgccggtatc ccgccttttg tccgtccgag ggtccgttcc cggctgtttc
      241 tgccgcctga tttttgcgca ggtggtcagg gagaggtccg cagggacggg caagaaaggc
      301 tctgttcaga atgatctgcg catcctggtc aaatccggaa tgcctgtccg tccagggaat
      361 cagtgctgaa cgtcgctgac atgtgactcc ggaacttgat gccggtatgc gacatggatt
      421 catccgcacc attggagcag gggacacgga cagggttgca ggtccggcag gtactactgc
      481 ccaagtctac cgaaacgtaa cagaatttcc gcattcatca cgaaaggaat acgcaccatg
      541 aagaagattc aggctctgaa gttgaccctg gcactgctgg cagcgggcat gagcatgctg
      601 gcaccggtca agcttgtgca tgctcagcag tattacaccc gctacatcta tgcgaacagt
      661 gtctgccggt atccgatccg catgatggtc taccacaagg acagcgaaaa taactaccac
      721 ccgcatggct ggtataattt ccgcccctac gaggaaagcc ggctgaaagc ccataatgtc
      781 gtgctgcgtc aggttgtcgg ctatgatctg tacttctatg ctgaaaccga caatcgttac
      841 aatgtgccgg ccatgtcctg ggcacgtggc gatgcggccg tcaacttcga aggtatttat
      901 tacaacctga cgcgtgccaa tctgttcgtg aacaccaaag gcgaactcga attcaaactc
      961 agttgcgcct gatgagtgaa gcgtatagga acggcagcaa ccggagttac cggactgagc
     1021 cgcccgcgac gaccgggcgg ctttttcatg ccggccccgg cgccgtgtat ccggacgcgg
     1081 gccgtttgcc gtggactgcg gagggacccg ggccttgtct tcagtccgcc agccgccacg
     1141 gcgcgccgcg gtgcgcgacg aggatgtgct tgaaggcgtc cgggtccttg atgaacgtga
     1201 gttccccggg cgtcgggaac agcttgtcga ccagccgcga cagccagaag cgcagcgccg
     1261 cggcgcgcag cacgcgcggc cattcggccc gttcggcttc gctgaacggc cgaagcgccg
     1321 cataggcgcc gtacagcgcc gcggcgcgtt cgcgatccag cgtgccgtcg ggctgcgtgc
     1381 accagtcgtt gacggtgacg gccacgtcga aggccagcgc gtcggtgcag gcgtagtaga
     1441 agtcgatcag cccgcagagc cggtcgccgt cgaacagtgc gttgtcgcgg aacagatcgg
     1501 cgtggatcgt gccctgcggc aggcgcgagc gcgggtgttc ggtcagcgcc gcgagttcgt
     1561 cgttcagcag atgcgcctcg acgcgatcga gcagcggcag cacgcgctcg gcggcgctct
     1621 ggcaccagcg cgggccgcgg tcgttgtcgc gccgttgcgc gaagccctcg ccggccagat
     1681 gcagctgcgc gagcgcgcgg ccgatctcgc ggcactgcgc gaggctcggc gtgccgtgca
     1741 ggccgccgcc ggcgagcttg tgcaccagcg ccgccggctt gccggccagc ggcttcatgt
     1801 aatgcccctg acggtcggcg accgggttcg cgcccggcac gccgcgggcc gcgagccagg
     1861 ccatcaggtc gaggtaatac ggcacgtcct cgaagccgat cagctcgaac agcgtcagca
     1921 cgacctcggt gcgggtcgtg gtcaggaagt agttggtgtt cgagatgcct tcgctgatgc
     1981 cgcgatgcgc gacgtagtcg ccgatgtcgt agtcgcggag gaaggtttcg agctgggcgc
     2041 gatcgaccgg ggtgtagacg gacatggcag gtactttgcg ggcggctgga cagcaagagg
     2101 ccccgtacgc ggacggggcc tcgggatcgg ctctcagggt agcgcgctca ccagcgcagc
     2161 agcacccagc tcgggatggc gatgccggga tcgaggtcgt tgtggcgggt ttcgaggttg
     2221 ccgtcgccgt cgctgtcgat caggaagtag ggcaggccct tcttcggcgt caccttgatc
     2281 atgtacagcc ggccgttgat gcggtactcg gcgacggtgt cctcggcacg gcgcgtgatg
     2341 gtcacgtccg gcaccggctg ttcgtcgacg ccgctttccg gcagcgggct cagctcgggc
     2401 ggcttcgggg cgggttcatc ggcggccagc gcgaccgggc cggcggcggc gagcagcaga
     2461 acggacagca gcaggctgcg catcatctct gatccttggc gatcgggaag gaagggcgtg
     2521 ggattctaca ggtcgagcag gatttcctgc tcctcggccg aaggttcgaa gccccggctc
     2581 tcgtagtggg aaaatataac atcgacgacc tgctgcggat cgtccacaag ttgcagcagt
     2641 tccagatcac cgccatcgat catgccctcg ggcaccagcg tgtcgcgaaa ccacgcgagc
     2701 agcggcgacc agaacgggct gtgcacgagg atgatcggga tgcgccgggt cttgccggtc
     2761 tgcaccagcg tcatgatctc ggccatctcg tcgagcgtgc cgaagccgcc cggcagcacg
     2821 acgtaggccg aggcgtactt cacgaacatc accttgcgcg agaagaagtg ccggaagctc
     2881 agcgcgatgt tctggtacgg gttgccgagc tgctcgtgcg gcagctggat gttcaggccg
     2941 atcgacggcg aacgcccggc ataggcgccc ttgttgccgg cctccatgat ccccggcccg
     3001 ccgccggtga cgaccgaaaa gccggcgtcc gacagcagca ggccgatctg ctcggcgagc
     3061 acgtagtacg ggtggccggg ctgcgtgcgc gccgagccga agatgctgac cgagggccgg
     3121 atgcgcgcca gacgctcgaa gccttccacg aactcggcca tgatctggaa gatcttccac
     3181 gactcgcgcg tcagcagcgc gtcgttgacc ggcgccatgc cgggatggga ctgcttgttc
     3241 tcgggggaca tcgcgctgct ccggggtgat tttcgttttt gatctcgtcg ggcgtcaggc
     3301 ttcgccgtgc ggccggaccg gccgccgtgc gcgcagtccg cgcccgcttc ctccaactcc
     3361 agcttatgcc gctgcgcgcg agcctcctgc ccatgaccgt actgctcgtc gatggctcct
     3421 cgtatctgta ccgcgccttc catgcgctgc cgccgctgtc gacggcgcgc ggcgagccga
     3481 ccggcgccgt gcgcggcgtc atcgcgatgc tgcgcaacct cgtcacgcgc ctcgcgccct
     3541 cgcgcatcgc cgtggtgttc gacgccagcg gcccgacctt ccgcgacgcg atctatcccg
     3601 cctacaaggc ccaccgtccg ccgatgcccg acgagctgcg cgcgcagatc gaaccgctgc
     3661 acgcgctggt acgcgcactc ggcctgccgc tgctctgcga gcccggcgtc gaggccgatg
     3721 acgtgatcgg cacgctggcg cgcgccgcca ccgcggccgg tgagtccgtc gtcatcgcca
     3781 ccggcgacaa ggacctcgcg cagctcgtcg atgcgcgcat cacgctgctc gacacgatga
     3841 agcgcgatgg ccaggaaacc gtgctcgatc acgacggcgt catcgccaag ttcggcgtgc
     3901 cgccggcggg catcgtcgat ctgctggcgc tgatgggcga ttcggtcgac aacatccccg
     3961 gcgtgcccgg cgttggcccg aagacggccg cgaagtggct cgccgagcac ggctcgctcg
     4021 cggcgctggt cgccggggcc gacggcatca agggcaaggc cggcgaggcg ctgcgcgcgc
     4081 atctggcgca gctcgacctg gcccggcagc tcgcgaccat ccgctgcgac gtgccgctgc
     4141 cgttcggcct cgaagccctc gccccgcagc cgcccgatcg cgccacgctg cgcacgctgt
     4201 acgagcggct ggaattccgc acctggctga aggagctcga cgctgccggc gaaccggttg
     4261 ccggtgccgc cgccgacagc gccccggccg ccgaagccgc gccgcccccg gcgccttctc
     4321 cggcacgcga ctacgaaacc gtgctcgacg aggccgcgtt cgccggctgg ctcgcccggc
     4381 tcgaagccgc cgatgccttc gcgttcgaca ccgagaccga cgcgctcgac tacatgcgcg
     4441 cgcgcatcgt cggcgtcagc ttcgcgatcg agccgcagcg cgccgcctac gtgccgcttg
     4501 cgcacgttta ccccggcgcg ccggatcagc tcgaccgtga cagcgtgctg gcgcgcctca
     4561 ggcccctgct cgaagacccg cacaagggca agatcggcca gcacgcgaag ttcgacatca
     4621 acgtgctcgc caaccacggc atcgcgctcg ccgggctggc cgacgacacc atgctcgaaa
     4681 gctacgtgct cgatgccacc gccacgcgcc acgacatgga ctcgctggcg gcgctgtacc
     4741 tcgatgaaaa gacgatcaag tacgaggacg ttgccggccg gggcgcgaag cagatcggct
     4801 tcgagcaggt gccgatcgag aacgccggcc cctacgcggc cgaggacgcc gacgtgacgc
     4861 tgcgcctgca cctggcgctg tcggcgaagc tcgccgaagt gccggcgctg atgggcgtct
     4921 accgcgacat cgagatgccg ctggtgccgg tgctgtcgcg catggagcgg cgcggcgtgc
     4981 gcatcgatca cgccgggctc cggcgcatga gcgccgagtt cgcgcagcgc ctgctcgaac
     5041 tcgaacgcga ggcgcacgat ctcgccggcc agcccttcaa cctcggctcg ccgaagcagc
     5101 tgcagagcat cctgttcgag cgccagcagc tgccggtgaa gaagaagacc ccgaccggcc
     5161 agccctcgac cgacgaggaa gtgctcgccg aactcgccga ggacggccac gcgctgccga
     5221 aggtgatcct cgaacaccgc tcgctcagca agctgcgcgg cacctacacc gaccgcctgc
     5281 ccgaactcgt caacccggtc accggccgcg tgcacaccag ctatcaccag gcggtcgcga
     5341 gcaccggccg gctgtcgtcc tccgacccga acctgcagaa catcccggtg cgcaccgacg
     5401 ccggccggcg catccgctcg gccttcatcg ccgagccggg ctgctgcgtg gttgccgccg
     5461 actattcgca gatcgaactg cggatcatgg cgcacctgtc cggcgacgcc gcgctgctcg
     5521 ccgccttcgc gcgcggcgac gacatccacc gggcgaccgc cgccgaggtg ttcggcctga
     5581 cgctggagca ggtcgcaccc gagcagcgcc gggccgccaa ggccatcaac ttcggcctga
     5641 tctacggcat gagcgccttc ggcctcgccc gccagctcgg catcgaacgc ggcgccgcgc
     5701 aggactacgt cagccgctac ttcgcgcgct atccgggcgt gaagaccttc atggacgaga
     5761 cccgccgctc ggccgccgcc cgcggctacg tcgaaaccgt gtccggccgg cgcctgtacc
     5821 tgcccgaaat caactcgaag aaccccgcgc gccgcgccgc cgccgagcgc accgcgatca
     5881 acgcgccgat gcagggcacg gccgccgaca tcatcaagcg cgcgatgatc gccgtggacg
     5941 gctggatcga agcggccggc gtgccggcgc gcatgatcct gcaggtgcac gatgaactgg
     6001 tgttcgaggt cgaggaggcc gcgctcgaag ccttctgcaa ggaggtgcgc acgcacatga
     6061 gcgccgccgc cgagctgtcg gtgccgctgc tggtcgaggt cggcagcggc aggaactggg
     6121 acgaggcgca ctga
//
LOCUS       DAOQIE010000245         6072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7131_length_6072_cov_7.635699, whole genome shotgun sequence.
ACCESSION   DAOQIE010000245 DAOQIE010000000
VERSION     DAOQIE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_6072_cov_7.635699"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK144_13965"
     CDS             complement(<1..267)
                     /locus_tag="PK144_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66880.1"
                     /translation="MAAGDTVMIGAAPVSCGSAAPGSGPAMSVAAAGLSAQMLAAATD
                     NTSTAPADGVDAGVVAGAVTALVGAAAAEGAVAAVKDIEAKASTD"
     gene            359..1021
                     /locus_tag="PK144_13970"
     CDS             359..1021
                     /locus_tag="PK144_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNF66881.1"
                     /translation="MQAAPVAAGLAEVLAAEAAPVDAGQSPSISGMPSVTTTPVAADA
                     GLNPAGVRGATASDAQAVPAPQAPLPVANPRAWAPLLGQHLMHLVQTGDTQATVRVNP
                     PQLGPVELRLELKDSLTHVSFYAHDAQVREALEQSLPRLRELLGAQGLQLGQSHVGDQ
                     ASQQQQQQPNPDGGSMAGSGDRRGNGRNDGMSGRDETAAVSGAEVVLPVRKLLGGVDH
                     YV"
     gene            1345..1857
                     /locus_tag="PK144_13975"
     CDS             1345..1857
                     /locus_tag="PK144_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNF66882.1"
                     /translation="MPQTMNWADYAILILIGLSMLLSLWRGFVREVISIVTWVLAFFL
                     AFNFSDLALAQLSHWVTLPETPSIRQLIGFATVFVGTLFVGGIVNLLIGQLVDGSGLG
                     PTDRMVGSLFGLARGVAIVALLVMLGSRTALVRDPWWQQSMLLPHFLPLAETLHSWLP
                     PEVASGVVFR"
     gene            1935..3446
                     /gene="purF"
                     /locus_tag="PK144_13980"
     CDS             1935..3446
                     /gene="purF"
                     /locus_tag="PK144_13980"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNF66883.1"
                     /translation="MCGIIGIVGHGPVNQVIFDGLTVLQHRGQDAAGMMTCDGGRLYL
                     RKNNGLVRDVIRTRHMLRLLGSMGIGHVRYPTAGSSDSAESQPFYVNSPFGIAMAHNG
                     NLTNAHRLREELFRSDRRHLNTDSDSEVLLNVFAHELARNGRLSVEADDVFGAVRRLH
                     QRVRGAYATVAMIIGYGVVAFRDPNGIRPLCYGQRETEDGVETLIASESVALDCLGFR
                     RVRDLEPGEAIFVSVDGQVFTRQCAEAPRHSPCIFEYVYFARPDSIIDDVPVHQARLA
                     MGEKLADKILHTWPDHDIDVVIPIPDTSRTAALSLAYHLGVQFAEGFMKNRYIGRTFI
                     MPGQKQRKKSVKQKLNPIPFEFKDRKVLLVDDSIVRGTTSGEIIRMAREAGAARVYFA
                     SAAPPVRFPNVYGIDMPAVEELIAAGRSEADVAAAIGADRLVYQDLDDLVASVRVANP
                     KLTQFDTSCFTGEYVTGDIDGQYLAKLERERNDLAKAGRDSEEHSVPELHNVN"
     gene            3766..4989
                     /locus_tag="PK144_13985"
     CDS             3766..4989
                     /locus_tag="PK144_13985"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNF66884.1"
                     /translation="MTADLIPELPDAADDAALAFASLAVRAGQSRSAEGEHSEAIYPT
                     SSYVFDSAAQAAARFAGEEPGNIYSRFTNPTVRMFEQRLAALEGGEACVATSSGMAAV
                     LTACMGLLKQGDHVVCSRDVFGNTTALLNNFLARFGVATTYVPLSDTAAWRAALRPET
                     KVLYLETPSNPLMELADIRELADIAHDHGALLVVDNCFCTPALQRPLALGADVVVHSA
                     TKYIDGQGRCVGGAVVGSKALIEKDIFTFLRNAGPTMSPFNAWVFLKGLETLPLRMRA
                     HSAAALELAQWLAVHPAVARVNYPGLETHPQHALARRQQSGFGGVLSFELEGGRAAAW
                     SVIDAVRMISITGNLGDTKSTITHPATTTHGKLSAEQKAAAGITEGLIRVSVGLEDVT
                     DIRRDLARGLDALAR"
     gene            complement(4997..5365)
                     /locus_tag="PK144_13990"
     CDS             complement(4997..5365)
                     /locus_tag="PK144_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66885.1"
                     /translation="MTAADHPSPALLAWEIYPEAGEQGCFVFAADRPAAVAAGAAELG
                     IAPEAVESVLRMPEFDAFAPGPIPLAALLEQGCEYECPVCGCRIAQGARDGNGRVLSP
                     VEAGDQVYCSATHAAQARHD"
     gene            complement(5425..>6072)
                     /locus_tag="PK144_13995"
     CDS             complement(5425..>6072)
                     /locus_tag="PK144_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNF66886.1"
                     /translation="GIDTIDLYYQHRVDPQVPVEETIGALAGLVAAGKVRYLGLCEVS
                     AATLARAHAVHPVTAVQSEYSLWTRDPEDEVLAACARLGCGFVPYSPLGRGFLTGALR
                     TPDDFAPDDYRRHSPRFQGENFAHNLRLVDEVRSLAAARGVTPAQLALAWVLAQGPHI
                     VPIFGTTRPENLRANLAALAIELDETTRQTLEQVFPRAAAAGLRYPQAMMGLLNA"
BASE COUNT          897 a         2149 c         2101 g          925 t
ORIGIN      
        1 atccgtgctt gccttcgcct cgatgtcctt cacggctgcc accgcgccct cggcggctgc
       61 agcgccgacg agtgcggtca cagcgcctgc gacaactccg gcgtccacgc catcagccgg
      121 cgcggttgaa gtgttgtctg tggctgccgc aagcatctgt gccgacagtc cggcggcggc
      181 caccgacatt gccgggccgc tgccgggcgc tgctgatccg caggacaccg gtgctgcgcc
      241 gatcatcacg gtgtcgccgg ccgccatgcg ccgatccgcg ccagccggtg atccgtcggc
      301 gtcacaggcc acagcgtccg gcattgccgc tcaggcgctg gcggcagccg gagcatccgt
      361 gcaggctgcg ccggtggcgg cggggctggc ggaagtcctg gccgcggaag ccgcgccggt
      421 cgacgccggg cagtcgcctt cgatttccgg gatgccttcg gtcacgacga caccggtcgc
      481 tgccgatgcc ggcctgaatc cggccggcgt gcgcggtgcg accgccagcg acgcgcaggc
      541 ggtcccggca ccgcaggccc cgctgccggt cgccaacccg cgcgcctggg caccgctgct
      601 cggccagcat ctgatgcacc tcgtgcagac cggcgacacg caggcgaccg tgcgcgtcaa
      661 tccgccgcag ctcggcccgg tcgagctgcg tctggagctg aaggactcgc tgacccacgt
      721 cagcttctat gcccacgatg cgcaggtgcg cgaggcgctc gaacagagcc tgccgcggct
      781 gcgcgagctg ctcggcgcgc agggcctgca gctcggccag agccacgtcg gcgaccaggc
      841 atcgcagcag cagcaacagc agccgaatcc ggacggcggc agcatggccg gctccggcga
      901 tcggcgcggc aacggacgaa acgacgggat gagcggacgc gacgagacgg ccgccgtgag
      961 cggcgcggaa gtcgtgctgc cggtgcgcaa gctgctcggc ggtgtcgatc attacgtctg
     1021 aaccgctctc tgaagcggcc tgaaccggcc tgcatcgcag ccatgccggc cgggtttccg
     1081 gcaccggtct tgtcgggcag gcggtgcacc cgcatttcgg cagtccgtga ggttgccgga
     1141 atcttcggat gctgatgccg atacgactgc cgttcctgcc gccgatggcg ctgtgccggt
     1201 ccgaaacgcc gccgctgcag cgctgtcgtg ccggatttac atcgctgcgc atggccccga
     1261 aggcatccgg gtttggacgg gccggccgct ctgatagaat ccgcgccctc gccgggcagc
     1321 cccgagccgg cacggaaact ccgcatgccg caaacgatga actgggccga ttacgccatc
     1381 ctgatcctga tcggcctgtc gatgctgctg agcctctggc gcggcttcgt gcgcgaggtg
     1441 atctcgatcg tcacctgggt gctcgccttc ttcctcgcct tcaatttttc cgatctggcg
     1501 ctggcgcagc tgtcgcactg ggtgacgctg ccggaaacgc cgtcgatccg tcagctgatc
     1561 ggtttcgcga ccgtgttcgt cggcacgctg ttcgtcggcg gcatcgtcaa cctgctgatc
     1621 ggtcagctgg tcgatggctc cggcctcggc ccgaccgatc gcatggtcgg ttcgctgttc
     1681 gggctggcgc gcggcgtggc gatcgtggcg ctgctcgtga tgctcggctc gcgcacggcg
     1741 ctggtgcgcg atccgtggtg gcagcagtcg atgctgctgc cgcacttcct gccgctcgcc
     1801 gagaccctgc acagctggct gccgccggaa gtggcgagcg gcgtcgtgtt ccgctaggca
     1861 gcgccggcgc cgagcgatcg atgattcccc ccaccggcgg ctgtgcggcc gtcccagccc
     1921 gagagtaagc aaccatgtgc ggaatcatcg gcatcgtcgg gcacggcccg gtcaaccagg
     1981 tgatcttcga cgggctgacc gtgctgcagc accggggtca ggacgccgcc ggcatgatga
     2041 cctgcgacgg cggccggctg tacctgcgca agaacaacgg tctggtgcgc gacgtgatcc
     2101 gcacccgcca catgctgcgc ctgctcggca gcatgggcat cggccacgtg cgctacccga
     2161 ccgccggcag ctcggattcg gccgaatcgc agccgttcta cgtgaactcg ccgttcggca
     2221 tcgcgatggc gcacaacggc aacctgacca atgcccaccg gctgcgcgag gaactcttcc
     2281 gcagcgaccg ccggcacctg aacaccgatt ccgattccga ggtgctgctg aacgtgttcg
     2341 cgcacgagct ggcgcgcaac ggccggctca gcgtcgaggc cgatgatgtc ttcggcgccg
     2401 tccggcgcct gcaccagcgc gtacgcggcg cctatgcgac cgtggcgatg atcatcggct
     2461 acggcgtcgt cgcgttccgt gacccgaacg gcatccggcc gctctgttac ggccagcgcg
     2521 agaccgagga cggcgtcgag acgctgatcg cctccgagag cgtcgcgctc gactgcctcg
     2581 gcttccggcg cgtgcgcgac ctcgaaccgg gcgaggcgat cttcgtcagc gtcgacggcc
     2641 aggtcttcac gcgccagtgc gccgaggccc cgcgccattc gccgtgcatc ttcgagtacg
     2701 tgtacttcgc gcggcccgat tcgatcatcg acgacgtgcc ggtgcatcag gcgcggctgg
     2761 cgatgggcga gaagctcgcc gacaagatcc tgcacacctg gccggaccac gacatcgatg
     2821 tcgtcatccc gatcccggac accagccgca ccgcggcgct gtcgctcgcg taccacctcg
     2881 gcgtgcagtt cgccgagggc ttcatgaaga accgctacat cggccgcacc ttcatcatgc
     2941 ccggccagaa gcagcgcaag aaatcggtca agcagaagct caacccgatc ccgttcgagt
     3001 tcaaggaccg gaaggtcctg ctggtcgatg actcgatcgt gcgcggcacg acctcgggcg
     3061 agatcatccg catggcgcgc gaggccgggg ctgccagggt ctatttcgca tcggccgcgc
     3121 cgccggtgcg cttcccgaac gtctacggca tcgacatgcc ggcggtcgag gaactgatcg
     3181 ccgccgggcg cagcgaggcc gatgtcgccg cggcgatcgg cgccgaccgg ctggtctatc
     3241 aggatctcga cgacctggtc gcgtcggtgc gcgtcgccaa tccgaagctc acgcagttcg
     3301 acacctcgtg cttcaccggc gagtacgtga ccggcgacat cgacggccag tacctcgcca
     3361 agctcgaacg cgagcgcaat gacctcgcca aggccgggcg cgactccgag gagcacagcg
     3421 tgccggagct gcacaacgtc aactgagcgt tccctgaacg cctgccgccg ggccgccgcc
     3481 gcgcttgccg gcggcggtgc gggcgggtag cttggcgccc cggtccgcag cctttgccgg
     3541 actcaggacc cgcggcggcc ctcgccgtcg tgtgcatcac ccggaccggg acggtccggc
     3601 gcgccgattt gtgcagcttg caggcgcgtt acaaatctcg ctaaagcgcg ggccggaccc
     3661 gactttagcg cgagctgatc gcggggcggg cggcccggtc gcccgccgca acttttccgg
     3721 gggcgcgcca gtaaagctgc gctcccgtac cgtggagcga cgcgcatgac cgcagacctg
     3781 atccccgagc ttccggatgc cgccgacgat gcggcgctgg ccttcgcctc gctggccgtg
     3841 cgtgccggcc agagccgcag cgccgaaggc gagcactccg aggcgatcta cccgacctcc
     3901 agctacgtgt tcgacagcgc cgcgcaggcc gccgcgcgct ttgccggcga ggaaccgggc
     3961 aacatctatt cgcgcttcac gaacccgacc gtgcgcatgt tcgagcagcg gctcgcggcg
     4021 ctcgaaggcg gcgaggcctg cgtggccacc agttcaggca tggcggcggt gctgaccgcg
     4081 tgcatgggcc tgctgaagca gggcgaccac gtcgtctgct cgcgcgacgt attcggcaac
     4141 accacggcgc tgctgaacaa cttcctcgcc cgcttcggcg ttgccaccac ctacgtgccg
     4201 ctgtcggaca ccgcggcctg gcgcgcggcg ctgcgccccg agacgaaggt gctgtacctg
     4261 gaaacgccgt cgaacccgct gatggagctg gccgatatcc gcgaactcgc cgacatcgcc
     4321 catgaccacg gtgcgctgct cgtcgtcgac aactgcttct gcacgccggc gctgcagcgg
     4381 ccgctggcgc tcggggccga tgtggtcgtg cattcggcga ccaagtacat cgacggccag
     4441 gggcgctgcg tcggtggcgc cgtggtcggc agcaaggcgc tgatcgagaa ggacatcttc
     4501 accttcctgc gcaacgccgg cccgaccatg agcccgttca acgcctgggt gttcctgaag
     4561 gggctggaaa ccctgccgct caggatgcgc gcgcattcgg ccgctgcgct cgaactcgcg
     4621 cagtggctgg ccgtgcaccc ggcggtggcg cgcgtcaact atcccggcct cgaaacccac
     4681 ccgcagcacg cgctggcccg ccgccagcag tcgggcttcg gcggcgtgct gagcttcgaa
     4741 ctcgagggtg gccgggcggc ggcgtggtcg gtgatcgacg cggtgcgcat gatctcgatc
     4801 accggcaacc tcggcgacac caagagcacg atcacgcacc cggcgactac gacgcacggc
     4861 aagctcagtg ccgagcagaa ggccgcggcc ggcatcaccg aaggcctgat ccgcgtgtcg
     4921 gtcgggctgg aggacgtgac cgacatccgc cgcgatctcg cgcgcgggct ggatgcgctt
     4981 gcgcgctgaa cctgcctcag tcgtggcgcg cctgcgcggc gtgcgtggcc gagcagtaca
     5041 cctgatcgcc ggcctcgacc ggcgacagca cccggccgtt cccgtcgcgc gcgccctgcg
     5101 cgatccggca gccgcacacc gggcattcgt attcgcagcc ctgttcgagc agcgccgcga
     5161 gcgggatcgg gccgggggcg aaggcatcga actcgggcat gcgcagcacc gattcgaccg
     5221 cttcgggcgc gatgccaagc tcggcggctc cggccgcgac cgctgccggc cggtcggcag
     5281 cgaacacgaa acagccttgc tcgccggctt cgggatagat ctcccaggcg agcagggcag
     5341 gggaggggtg atcggcggcg gtcattggca agggctccgg agcgggggcg tacaggataa
     5401 ccgccgtgct gccgggcgca cggatcaggc gttcagcagc cccatcatcg cctgcggata
     5461 acgcaacccg gctgcggccg cgcgcgggaa gacctgttcc agcgtctggc gcgtggtctc
     5521 gtccagttcg atggcgaggg ccgccaggtt ggcgcgcagg ttttcggggc gcgtcgtgcc
     5581 gaagatcggc acgatgtgcg gcccctgcgc cagcacccag gccagcgcga gttgcgccgg
     5641 cgtcacgccg cgggccgccg ccagactgcg cacttcatcg accagacgca ggttgtgcgc
     5701 gaagttttcg ccctggaagc gcgggctgtg gcggcggtag tcgtcgggcg cgaagtcatc
     5761 gggcgtgcgc agcgcgccgg tcaggaagcc gcgcccgagc gggctgtagg gcacgaagcc
     5821 gcagccgagc cgcgcacagg cggcgagcac ctcgtcctcg gggtcgcgcg tccacagcga
     5881 gtactcgctc tgcacggccg tgaccggatg cacggcatgg gcgcgcgcca gcgttgcggc
     5941 cgaaacctcg caaaggccca ggtagcggac tttgccggcc gcgaccagcc cggcgagcgc
     6001 gccgatggtc tcctcgacgg gcacctgcgg atcgacgcgg tgctggtaat agagatcgat
     6061 cgtgtcgatg cc
//
LOCUS       DAOQIE010000246         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7167_length_6053_cov_7.758419, whole genome shotgun sequence.
ACCESSION   DAOQIE010000246 DAOQIE010000000
VERSION     DAOQIE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_6053_cov_7.758419"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK144_14000"
     CDS             <1..469
                     /locus_tag="PK144_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66887.1"
                     /translation="LEAKLIEALKAYPFTVENVALREIGLPAVVQKKIEEVQLAKQEE
                     QRLAMVEQQARKEQEIKTIQANTRLVETTTDARAQAERRKLEADANAYQIAKEAEARA
                     TANQKIAASLTAELVHYQSVLQWNGVYPATLVQGGGPGLLLNLPAGGPAAAPR"
     gene            678..1655
                     /locus_tag="PK144_14005"
     CDS             678..1655
                     /locus_tag="PK144_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66888.1"
                     /translation="MTTQSVPARLPDFAPAPEPGTRRIIDQQERVYYEGYWIKTYPVP
                     GDCLAAKKNLIEALTRRLFNHTEHGLNVPGVRLQEARAAYEAETDPALRRVKGGMLAG
                     ALFNRATDIFRRLVELQADGVEIQTDYPLVQECGHCLLEALELGRLVRHRSGQECIDE
                     LWGEPFRVFSLSVEEFFESRYLKIGMCLRDIDRIAAAMIEHLQPIPVFEGLAPLVSDF
                     AEAARIKTETLRTDARIFDVWSRFVTAGERLAAYEPGLPAARATRTPGRRVHGLVDGM
                     RLVRAGRSLIFHIVRARSPMPKSMADYLAHLAFYRDKGRISPTLPVMPE"
     gene            complement(1675..2094)
                     /locus_tag="PK144_14010"
     CDS             complement(1675..2094)
                     /locus_tag="PK144_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66889.1"
                     /translation="MPELRALLFPPEPRRFAGQRWVNVALRSVHLCAVAGLGAGFLHG
                     LPPASWQPWLWVALASGSALGLLFVWSDGRWLVQPPGLAVLAKLGLLGLTPFVPAAGA
                     WLFLGAVALSSVFAHAPARVRHCLLLPARWVPPQVRR"
     gene            complement(2379..>2954)
                     /locus_tag="PK144_14015"
     CDS             complement(2379..>2954)
                     /locus_tag="PK144_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66890.1"
                     /translation="LMGLFFDLAPPYTLSSPTATRSAGSSYVNLATPSSSTNLGGEWA
                     YRYSATGLSGSTVTAKYGVSGSGLGGTFGAGDRICSACPELSSGGGSAPDGLDYGIVS
                     NSYVANTGSGVTSTVELVRNSISFMFNVAGGSILNLGAVISNVSFQYGTALDEPRLAG
                     VKISEPASAALLALGGLLSCRKRRAMAGSAA"
     assembly_gap    2955..3054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3055..3337)
                     /locus_tag="PK144_14020"
     CDS             complement(<3055..3337)
                     /locus_tag="PK144_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66891.1"
                     /translation="MIASTLHAHRALLLAGVLAAASTGAGASVSFVATGPNTYSGGHT
                     LAAQAIFDLSGSVLSITLKNISTDTLDRPSDVLMGLFFDLAPPYTLSSPT"
     gene            complement(3570..3800)
                     /locus_tag="PK144_14025"
     CDS             complement(3570..3800)
                     /locus_tag="PK144_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66892.1"
                     /translation="MGSKLTTTARLDVSVPVLCRRQSVDTRLNRRIGVRIHFSLRIPA
                     AGSDCLDDEGMRVKKFYILLIVQMQICAASKA"
     gene            4174..4419
                     /locus_tag="PK144_14030"
     CDS             4174..4419
                     /locus_tag="PK144_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66893.1"
                     /translation="MNKKLICIGVFGVSLVWFDSFFAALIIVGFFAVIALVLLPASSI
                     DLGFDEWGSDESSGSGGDREGGGDSADSGGDWGGGCD"
     gene            complement(4621..>6053)
                     /locus_tag="PK144_14035"
     CDS             complement(4621..>6053)
                     /locus_tag="PK144_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNF66894.1"
                     /translation="TVKGDVHDIGKNIVGVVLQCNNFEVFDLGVMVPADRILAKAKEV
                     GAHIIGLSGLITPSLDEMVNVAKEMQRQQYALPLLIGGATTSVMHTAVKIDPQYEQAV
                     VYVKDASRAVGVAQSLVSPELKPEFVAKTKADYASKREQHRLRRNREALLPFAQARAN
                     ALAPDPADGAPLAPRVPGVQVLLDYPLAELVEYIDWTPFFSAWELAGKFPRILDDAVV
                     GEQARKLFDDAQTMLRRIVDERWLSANAVIGLFPANRDGEDTIVWTDETRTTERTRMH
                     HLRQQSVRPDGKPNQSLADYVAAPGTPDWVGAFACTAGIGIDAHVARFEAAHDDYSAI
                     LLKALADRLAEAFAERLHERVRREWWGYAPEEALANEQLIDEAYRGIRPAPGYPACPD
                     HTEKATLWTLLDVEAQAGITITESFAMVPTAAVSGWYFAHPRAHYFGLGRITRDQVED
                     YARRKGWELATAEKWLAPSLGYEAEE"
BASE COUNT         1020 a         1890 c         2066 g          977 t
ORIGIN      
        1 gctcgaagcg aagctcatcg aggcgctgaa ggcctatccg ttcacggtcg agaacgtcgc
       61 gctgcgcgag atcgggctgc cggcggtcgt gcagaagaag atcgaggagg tgcagctcgc
      121 caagcaggag gagcagcgcc tggcgatggt cgagcagcag gcgcgcaagg agcaggagat
      181 caagaccatc caggccaaca cgcgcctggt cgagaccacg accgacgcgc gcgcgcaggc
      241 cgagcgcagg aagctcgaag ccgatgccaa cgcctaccag atcgcgaagg aggccgaggc
      301 ccgcgccacc gccaatcaga agatcgccgc gtcactgacc gccgaactcg tgcactacca
      361 gtcggtgctg cagtggaacg gcgtgtatcc ggcgacgctg gtgcagggcg gcgggccggg
      421 tctgctgctg aacctgccgg ccggcgggcc ggcggccgca ccgcgctgaa ggccgggcgc
      481 ggtccgccgc ggccgtgccg cggcgaacgg ctgcggtgca ccaccgcctg ccctgcgcgg
      541 cgtgcactgc accggcgcag tgcactcatg cggccgcccg cccgcccgga tcgggagcgg
      601 aatcaagggg ctgggcgcca ccgcagtgca gcacgcttct tgctggaaaa gccccggttg
      661 aaccgccggg gatcctcatg acgacgcaat ccgttccggc ccgcctgccc gacttcgcgc
      721 cggcgcccga gccgggcacc cgccgcatca tcgatcagca ggagcgggtg tattacgagg
      781 gctactggat caagacctat ccggtgccgg gcgactgtct ggcggccaag aagaatctga
      841 tcgaggcgct gacgcggcgc ctgttcaatc acaccgaaca cggcctcaac gtgcccggcg
      901 tgcgcctgca ggaagcccgg gcggcgtacg aggcggaaac cgatccggcg ctgcggcgcg
      961 tcaagggcgg catgctggcc ggggcgctgt tcaatcgcgc caccgacatc ttccgccggc
     1021 tcgtggaact gcaggcggac ggcgtggaaa tccagacgga ttacccgctg gtgcaggaat
     1081 gcggccactg cctgctggag gcgctggagc tggggcggct ggtccggcat cgcagcgggc
     1141 aggaatgcat cgacgagctg tggggcgagc ccttccgcgt gttctcgctg tcggtcgagg
     1201 agttcttcga aagccgctat ctgaagatcg gcatgtgcct gcgcgacatc gaccgcatcg
     1261 ccgccgccat gatcgaacat ctgcagccga tcccggtgtt cgagggtctg gcgccgctgg
     1321 tcagcgactt cgcggaagcg gcgcggatca agaccgagac cctgcgcacc gatgcgcgga
     1381 tcttcgacgt gtggtcgcgc ttcgtcaccg ccggcgagcg tctggcggcc tacgaaccgg
     1441 gcctgcccgc ggcccgggcg acgcggaccc cgggccggcg cgtgcacggg ctggtggacg
     1501 gcatgcgtct ggtgagggcg ggccgctccc tgatcttcca catcgtccgt gcccgctcgc
     1561 cgatgccgaa gagcatggcc gactatctcg cccatctggc cttctaccgg gacaaggggc
     1621 gcatctcgcc gacgctgccg gtcatgccgg aataggccgg gggcacgccg ccggctagcg
     1681 ccggacctgc ggcggcaccc agcgcgccgg cagcagcagg caatggcgca cgcgcgccgg
     1741 ggcgtgcgca aacaccgacg agagcgcgac cgccccgagg aacagccagg ccccggccgc
     1801 cggcacgaac ggcgtcagcc cgagcagccc gagcttcgcc agcaccgcca gccccggcgg
     1861 ctgcaccagc cagcgcccgt cgctccacac gaacagcagc cccagcgccg aaccgctcgc
     1921 cagggccacc cacagccagg gctgccagct cgccggcggc agcccgtgca ggaagccggc
     1981 cccgagcccg gcgaccgcgc acagatgcac gctgcgcagc gcgacgttga cccagcgctg
     2041 gccggcgaag cggcgcggtt cgggcggaaa cagcagggcg cgcaactcgg gcatcggcgg
     2101 atcgcaacag cggggcgggg cgcgcatgtt cggtcgaggc aggcagcggc gtccatccgc
     2161 cggaaggggc ggacgatgcg gcggattgat ccgcagattg caggcgtgcc gcggttgccg
     2221 gggcaggggc gaatgacaga aaccccggag cgctgccgcg catccggggt tcatgcggtc
     2281 atggtgctgc gctggatcgc gccgccgccg ggcagcgcgc cttcatttcc agcccgcctg
     2341 cctcgcggca cgaggaagcc ggggcggaca ttccggattc aggctgctga cccggccatt
     2401 gcgcgccgtt tccggcatga aagcagtcca cccagcgcca gcagtgcagc acttgccggt
     2461 tcggagattt tgactccggc aagtcgtggt tcgtcgagtg ccgtgccgta ctggaagctg
     2521 acgttgctga tcacggcgcc gagattcagg atgctgccgc ccgccacgtt gaacatgaat
     2581 gaaatgctgt tgcgcacgag ttcgacagtg gacgtcacac cgctgccggt attggcgacg
     2641 taactgttcg atacgatgcc atagtcaaga ccgtccggtg ctgaaccacc gcctgaactc
     2701 agttcgggac aggcgctgca gatccggtcg ccggcaccga aggtgccgcc gagtccggaa
     2761 ccggaaaccc cgtatttggc agtgacggtc gatccggaca atccggtcgc cgaataccgg
     2821 tacgcccatt caccgccgag attggtgctc gacgacggtg tggcgagatt cacgtaggaa
     2881 ctgccggcac tccgcgtcgc cgtgggcgat gacagcgtgt agggcggtgc gagatcgaag
     2941 aacagtccca tcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgtgg
     3061 gcgatgacag cgtgtagggc ggtgcgagat cgaagaacag tcccatcagt acatccgagg
     3121 ggcgatcgag agtgtctgtc gagatgtttt tcagcgtgat cgacagcacg ctgcctgaca
     3181 gatcgaaaat cgcctgtgcc gccagcgtgt gtccgcccga ataggtattg ggaccggtcg
     3241 ccacaaaact gacggaggct ccggcaccgg tactcgcggc agcgagtacg ccggccagca
     3301 gcaacgcgcg gtgcgcgtgc agggttgatg caatcatcag aggtcccctc cgcgaagttc
     3361 accaggaacg tgggcggcgg ggcagagggt gcgggtacct tccgggtggg gaatttcacc
     3421 ccgtaaacaa acagtcgctt ccgtgtgtat ccgtgttcaa ccgaccggca tctgcgccgc
     3481 ggcatgtacc ggggggctgc gactgcccgc ccggctggta aggcagcaaa aaatgttgca
     3541 atggttatgc cggtcacggc aagtgcttgt caagctttgc ttgcggcgca gatttgcatc
     3601 tgaacaatca gtaatatgta aaattttttg acacgcatgc cttcatcatc aaggcaatcg
     3661 ctgccggcag caggaatacg caaggagaaa tgaatccgga cgccgatgcg gcggttcagc
     3721 cgggtatcaa cagactgacg gcggcagagg accggaacgc tgacatcaag ccttgctgtc
     3781 gtcgtcagct tcgatcccat ggcaagcgca ccttcccggc atgcgaaaag tcaggggctg
     3841 cgtgggtggc aggcgcttga tcattttgtt caggcacggc ttgcatgacc gccgattttc
     3901 gcctgaactg cacgcaggcc ggcagtcatg ttgcgctgca agaagatgtg ttcgttctgc
     3961 ggcaggcttg caagcccggc atgtggcgga tggcattgag accatgtccg ggtttcatcg
     4021 tcactcagca ctgcatgctg ttcaggaagt tgtgctggtc aacggaggtt tcatgaatat
     4081 ccatgaatcg tggcctgatg tgtcgatgga tgtgatatgc gcaaaggcgt atccggaatt
     4141 cttgtcctca ggcatgcgcg gagtctcgca gaaatgaaca aaaaactcat ctgcatcggc
     4201 gtgttcggag tttctctcgt atggttcgac agtttttttg cagccctcat catagtgggc
     4261 ttcttcgcag tcattgcgct ggtgcttttg cctgcatcgt cgattgatct cgggtttgac
     4321 gaatggggca gcgacgaaag ctctgggtct ggtggagacc gggagggtgg tggagacagt
     4381 gccgattcgg gtggggattg gggcggcggg tgtgattaag tgcgcgcagc cattgccggc
     4441 gatggcgacc cggcagcctg gtgcggatgc cctgcgtcga acaaccggaa gttacatcag
     4501 gcgcgcattc atgcgcacgc agtcaaacgt caaatgtcca tgtttccggt aatgcgcacc
     4561 ctgccggaga tttcagggtg cgcacgccac tgaattacgg gcgggacatc cgcccccgcc
     4621 tcattcctcg gcctcatacc cgaggctcgg cgccagccat ttctccgccg tcgccagttc
     4681 ccagcccttg cgccgtgcgt aatcctcgac ctgatcgcgc gtgatgcggc cgaggccgaa
     4741 gtaatgcgcg cgcgggtggg cgaagtacca gccggagacc gcggcggtcg ggaccatcgc
     4801 gaagctttcg gtgatggtga tgccggcctg cgcctcgaca tcgagcaggg tccacagcgt
     4861 cgccttctcg gtgtgatccg ggcaggccgg ataaccgggg gcggggcgga tgccgcggta
     4921 ggcttcgtcg atcaactgct cgttggcgag cgcctcctcg ggcgcgtagc cccaccattc
     4981 cctgcgcacg cgctcgtgca gacgctcggc gaaggcttcg gccagacgat cggccagcgc
     5041 cttcagcagg atggcgctgt agtcgtcgtg cgcagcctcg aagcgcgcga cgtgggcgtc
     5101 gatgccgatg ccggccgtgc aggcgaaggc gccgacccag tcgggcgtgc cgggcgctgc
     5161 cacgtagtcg gccagcgact ggttcggctt gccgtcgggg cgcaccgact gctggcgcag
     5221 gtggtgcatg cgcgtgcgct cggtcgtgcg cgtttcgtcg gtccagacga tggtgtcctc
     5281 gccgtcgcgg ttggccggga acaggccgat gacggcgttc gccgacagcc agcgttcatc
     5341 gacgatgcgc cggagcatcg tctgcgcgtc gtcgaagagc ttgcgcgcct gctcgccgac
     5401 cacggcatcg tcgaggatgc gcgggaactt gccggccagt tcccaggccg agaagaacgg
     5461 cgtccagtcg atgtactcga ccagctccgc gagcgggtag tcgagcagca cctgcacgcc
     5521 gggcacacgc ggggcgagcg gggcgccatc ggccgggtcg ggcgcgagcg cgttggcgcg
     5581 ggcctgcgcg aagggcagca gggcttcgcg gttgcggcgc aggcggtgct gctcgcgctt
     5641 gctggcgtag tcggccttgg tttttgcgac gaactccggc ttcagctcgg gactgaccag
     5701 cgactgcgcg acgccgactg cgcgcgaggc gtccttcacg tagacgacgg cctgctcgta
     5761 ctgcgggtcg atcttgaccg cggtgtgcat gaccgaggtg gtcgcgccgc cgatcagcag
     5821 cggcagcgcg tactgctggc gctgcatctc cttcgcgacg ttgaccatct cgtcgagcga
     5881 gggcgtgatc aggccggaca ggccgatgat gtgcgcgccg acctccttcg ccttggcgag
     5941 gatcctgtcg gccggcacca tgacgccgag gtcgaagacc tcgaagttat tgcactgcag
     6001 cacgacgccg acgatgttct tgccgatgtc gtgcacgtcg cccttgacgg tcg
//
LOCUS       DAOQIE010000247         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7180_length_6043_cov_7.839512, whole genome shotgun sequence.
ACCESSION   DAOQIE010000247 DAOQIE010000000
VERSION     DAOQIE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7180_length_6043_cov_7.839512"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PK144_14040"
     CDS             complement(<1..948)
                     /locus_tag="PK144_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNF66895.1"
                     /translation="MSPELPITAVLPALAQTLAAAPAVVLQAPPGAGKSTGVPLALLD
                     APWLAGRRIVMLEPRRLAARAVAARMSALLGEPPGATVGYRVRFEARVSARTRIEVVT
                     EGILTRRLQSDPELAGVGLLIFDEFHERSLHADLALALARDAQLGLREDLKLLLMSAT
                     LDGERLCRLLDDAPLVSSEGRAHPVAIRHVAREPEAPLPEALAQQVVRLLAEESGDVL
                     AFLPGGAEIRRAAERLRTLAPQVSVLPLYGELPAAEQQRALEPAPDGRRRVVLATTIA
                     ETSLTIDGIRLVVDGGWTRAPRFDAASGLTRLATVRVSRA"
     gene            1170..2264
                     /locus_tag="PK144_14045"
     CDS             1170..2264
                     /locus_tag="PK144_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66896.1"
                     /translation="MKTALVVDDSRLARLALTRALEKHGLHVEQVGSAAEAFEFLRGR
                     RPDVVFMDVTMPEMDGFTAAGLITRDPQFAGLPVVMCSAEETDDVRTRTAAAGAMAFL
                     GKTAGDAGLSDILNRLVPEAASMPAAAPALAAAGPSAAELAAQIESSVAAAVASRLVA
                     TEAALRQAIEAAVANAAREAVQSMAPALVQDLVYAAVPEAVRSEVSDRLPLVQSAARD
                     AALAVAEDAAGASAGRVRDLAASIVEERIAQGRTEWLHSLDASIESGPLAARIGQMAR
                     DAAQQGARSAAESLFAQVREGLMSELRGELGTLRGRVGDAPTREQLDEALAAAHKAEA
                     HAEATARQMRIGMVLLGLGIVASVAIGVLL"
     gene            2473..5118
                     /locus_tag="PK144_14050"
     CDS             2473..5118
                     /locus_tag="PK144_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF66897.1"
                     /translation="MARAQRKANIEATSAGNADERRSWLPALATLIGGLLLTAAIFLL
                     LASGERERAHDEFERAARDRFLSVARELEQGLTGLQGLAAFVYTNPGMSAEAFDALSR
                     HLLGLSPGLDTLAWAARDTQGGGDRYLLSRLYPAEGASLVAGADLLARPEFAATVLAA
                     RDGGRLALSEPFPRGGDGESRGVLAAVPVYRTLISGADERRRRQDIVGVAIAVLDPQR
                     LVEGALSRLDPVKVELRLFDLGAPRGRQLLYHPLGSLRGAAMASYLADPEAFGAVEHL
                     QRRIEIAQREWLVVATPAPGQFFASLGSRPWWALGVGLALTLAVAFWLRATVGRHGQV
                     EAMVALREAELSESALRFHQLTEGAPLVLWISSIENGQMLYLSPAFESIWGFTPEQVF
                     QRPGLRRELVHPEDRDRLRRLVRSAAQNEGYDTEYRIQRQDGELRWIRERAHAVRNAA
                     GVVYRLAGLSEDVTAQHRHAAEAAVRDARLRASFESLPEAVLIVGSSGCIESMNPAAE
                     RQFGYAADELLGGPVAVLLPGYDPLAPLDPSRPLSGVRRGGGRFPALVPSSDYDDDNG
                     RHHVCLVHDLTALGQSQTVSSRERERAQAILSSITEAVVTTDALGRVDFLSPLAERMT
                     GWNSAQALGQPLPSVLRLRDPLSGLERGDLMLSAGASEVLRLVRRDGSELAVHCSVAP
                     IRSLGQDVGGVVLVLRAAGSDAPGEVQAGQPSEPSEVLDSFGRDALTGLPDRPGFVAK
                     LDNALEAVQAGGAPRTLIYLTLRQMHSLTALGGEAMADRVRAGAAQALIDVLGPRDVA
                     GRVGADEFALLLEGWNAETLPEQLAEIRAALGQYSMEVQGRTHRVDADIGAIPVNADS
                     GDSLDVMSAADLACHRARVAARSGG"
     gene            complement(5126..5926)
                     /locus_tag="PK144_14055"
     CDS             complement(5126..5926)
                     /locus_tag="PK144_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNF66898.1"
                     /translation="MSAFGLDDFLWRALAGGLGVALAAGPLGSFVVWRRMAYFGDTLA
                     HAGLLGVVLGVLAGIGEGFGVLLTGVLLALLLLALQQQKRLPGDTVLGILAHTALSLG
                     LVAAAFLEQVRIDLNAWLFGDILAIAPGELAWIWAGGALALGVLALIWRPLLAMTVHE
                     ELARVEGVPVAAVRLVFLLLIALVVAVAMKVVGVLLITSLLIIPPAAARRWARSPEGM
                     ALGASLCGALAVSGGLAGSLAWDTPAGPSVVVCAALLFALGLLLPVRR"
BASE COUNT          820 a         2160 c         2298 g          765 t
ORIGIN      
        1 ggcgcggctg acgcgcacgg tcgccagccg cgtcagaccg ctggcggcat cgaagcgcgg
       61 cgcgcgtgtc cagccgccgt cgacgacgag gcggatgccg tcgatggtca ggctggtctc
      121 ggcgatcgtg gtcgcgagca ccacgcgccg gcggccgtcg ggagcgggtt ccagcgcacg
      181 ctgctgctcg gcagccggca gctcgccgta gagcggcagc acgctgacct gcggcgccag
      241 cgtgcgcagg cgctcggcgg cgcggcggat ctcggcgcca ccgggcagga aggcgagcac
      301 gtcgccggat tcctcggcga gcagccgcac gacctgctgc gccagcgcct cgggcagcgg
      361 ggcttcgggt tcgcgcgcga cgtggcggat cgcgaccgga tgcgcgcggc cttcgctgct
      421 cacgagcggc gcatcgtcga gcagccggca gagccgctcg ccgtcgagcg tggccgacat
      481 cagcaggagc ttcaggtcct cgcgcaggcc gagctgcgcg tcgcgcgcca gcgccagcgc
      541 cagatcggcg tgcaggctgc gttcgtgaaa ctcgtcgaag atcagcaggc cgacgccggc
      601 aagctccgga tcggactgca gccggcgggt caggatgcct tcggtgacga cctcgatgcg
      661 cgtgcgcgcc gagacccggg cctcgaagcg cacgcggtag ccgaccgtgg cgccgggcgg
      721 ttcgccgagc agcgcgctca tgcgcgctgc gaccgcgcgg gcggccaggc ggcgcggttc
      781 gagcatcacg atgcgccggc ccgcgagcca cggcgcatcg agcagcgcga gcggcacgcc
      841 ggtgctcttg ccggcgccgg gcggggcctg cagcacgacg gccggggccg cggcgagggt
      901 ctgtgccaga gccggcagca cggccgtgat cggcagttca ggggacatgg tgcttacaaa
      961 gcccttgcaa aagacgtgtc caggtggcag gcggctgggg cgtggagtgt aaaacatcgt
     1021 caaacagggg cgcggtgaaa atcccggaca tgggaaaagt ggctcgctat ggttctaatc
     1081 gcccggccac agggagcccc gcaccggagt gcggaagggc ggcagggaac atgattcgga
     1141 tcgggactgg atcggtgagg aaatcgacga tgaagacagc gttggtcgtg gacgattcgc
     1201 gactcgccag gctggcgttg acgcgcgccc tcgagaagca cgggctgcac gtcgaacagg
     1261 tcggctcggc cgcggaagcc ttcgagtttc tgcggggccg ccggccggat gtggtgttca
     1321 tggacgtgac gatgccggag atggacggct tcacggccgc cggcctgatc acgcgcgatc
     1381 cccagttcgc cgggctgccg gtggtgatgt gctcggccga ggaaaccgac gacgtgcgca
     1441 cccgcaccgc tgcagccggc gccatggcct tcctcggcaa gacggccggc gatgccgggc
     1501 tgtcggacat cctgaaccgt ctggtgccgg aggcggcgtc gatgccggcg gccgcgccgg
     1561 cgctggcggc ggccggaccg agcgcggcgg aactcgccgc gcagatcgaa tcctcggtgg
     1621 cggcggcggt cgcctcgcgg ctggtggcga ccgaggctgc gctgcgtcag gccatcgagg
     1681 ccgcggtcgc gaacgccgcg cgcgaggcgg tgcagtcgat ggcgccggcg ctggtgcagg
     1741 atctcgtcta tgccgccgtg cccgaggccg tgcgcagcga ggtcagcgac cggctgccgc
     1801 tggtgcagtc ggcggcgcgc gacgcggcgc tggcggtggc cgaggatgcc gccggggcca
     1861 gtgccggacg ggtgcgcgat ctcgccgcca gcatcgtcga ggagcgcatc gcgcagggcc
     1921 gcaccgaatg gctgcacagc ctcgatgcct cgatcgaatc cggaccgctc gctgcgcgca
     1981 tcggccagat ggcgcgcgac gccgcccagc agggcgcgcg ctcggccgcc gaatcgctgt
     2041 tcgcgcaggt gcgggaaggg ctgatgagcg agctgcgcgg cgagctcggc acgctgcgcg
     2101 gccgcgtcgg cgatgccccg acccgcgaac agctcgacga ggcgctcgcc gctgcgcaca
     2161 aggccgaggc gcacgccgag gcgacggccc ggcagatgcg catcggcatg gtcctgctcg
     2221 gcctcggcat cgtcgcctcg gtggccatcg gcgtgctgct ctgaggctgc agcgctgatc
     2281 cgaccccgac cccgcgcccg gcgcggggtt ttcgttgctg gcggcggccg gcgcgccggc
     2341 ggcggacgat aatcgcgccc ggtgccggcg ccggaacggg cagcagcgcc tgccggcggc
     2401 cgggaaggat gttctggagt ccgtctcaat gcacggccgt gcggccggtg cagccagcga
     2461 ggatcacgag gcatggcgcg tgcgcagcgg aaagcaaaca tcgaggccac gagcgcaggg
     2521 aacgccgacg agcggcgatc gtggctgccg gcacttgcga cgctgatcgg cggactgctg
     2581 ctgaccgcgg cgatcttcct gctgcttgcg agcggcgagc gcgagcgtgc ccacgatgag
     2641 ttcgagcgcg cggcgcgcga ccgcttcctg tcggtggcgc gcgagctgga gcagggcctg
     2701 accggcctgc aggggctcgc ggccttcgtc tacaccaacc ccgggatgtc ggccgaggcc
     2761 ttcgatgcgc tgtcgcgcca cctgctgggt ttgtccccgg ggctggacac gctggcctgg
     2821 gcggcgcgcg acacgcaggg cggcggtgat cgctacctgc tgtcgcggct gtacccggcc
     2881 gaaggcgcga gcctggtcgc cggtgccgat ctgctcgccc ggcccgagtt cgccgccacg
     2941 gtgctcgcgg cgcgcgacgg cggccggctg gcgctgtccg agcccttccc gcgcggcggc
     3001 gacggcgagt cgcgcggcgt gctggcggcg gtgccggtgt accgcacgct gatcagcggc
     3061 gccgacgaac gccgtcgccg tcaggacatc gtcggcgtcg cgatcgccgt gctcgatccg
     3121 cagcgcctgg tcgagggcgc gctgtcgcgg ctcgatccgg tcaaggtcga gctgcgcctg
     3181 ttcgatctcg gcgcaccgcg cggccgccag ctgctgtatc acccgctcgg cagcctgcgc
     3241 ggtgcggcga tggcgagcta tctcgccgat cccgaagcct tcggcgcggt cgagcacctg
     3301 cagcgcagga tcgagatcgc ccagcgcgaa tggctggtgg tggccacgcc ggcgccgggg
     3361 cagttcttcg cctcgctcgg cagccggcca tggtgggcgc tcggcgtcgg actggcgctg
     3421 acgctggcgg tggcgttctg gctgcgggcg acggtcggcc gtcacggcca ggtcgaggcg
     3481 atggtcgcgc tgcgcgaggc cgagctgtcc gagagcgcgc tgcgcttcca tcagctcacc
     3541 gaaggcgcgc cgctggtgct gtggatctcc agcatcgaga acggccagat gctgtacctg
     3601 agcccggcct tcgaatcgat ctggggcttc acgcccgagc aggtgttcca gcgcccgggc
     3661 ctgcgccgcg aactcgtgca tccggaggat cgcgaccggc tgcgccggct ggtgcgcagc
     3721 gccgcgcaga acgagggcta cgacaccgag taccgcatcc agcgccagga cggcgagctg
     3781 cgctggatcc gcgagcgcgc ccacgccgtg cgcaacgccg ccggcgtcgt ctaccggctc
     3841 gccggcctgt ccgaggacgt gaccgcgcag caccggcacg cggccgaggc cgcagtgcgg
     3901 gatgcgcgcc tgcgcgcctc cttcgagtcg ctgcccgagg ccgtgctgat cgtcggttcc
     3961 agcggctgca tcgagtcgat gaatccggcg gccgagcgcc agttcggtta tgcggccgac
     4021 gaactgctcg gcggaccggt cgcggtgctg ctgccgggct acgatccgct ggcgccgctc
     4081 gatccgtccc ggccgctgag cggcgtgcgc cgcggcggcg ggcgcttccc ggcgctggtg
     4141 ccgagcagcg attacgacga cgacaacggt cgccatcacg tctgtctggt ccacgacctg
     4201 accgcgctcg gccagagcca gaccgtcagc tcgcgcgagc gcgagcgcgc gcaggcgatc
     4261 ctttcgagca tcaccgaggc ggtcgtcacc accgatgcgc tcggacgcgt cgacttcctg
     4321 agtccgctcg ccgagcgcat gaccggctgg aacagcgccc aggcgctcgg ccagccgctg
     4381 ccctcggtcc tgcgcctgcg cgatccgctg agcggtctcg aacgcggcga cctgatgctg
     4441 tcggccgggg ccagcgaggt gctgcgcctg gtgcgccgcg acggcagcga gctggcggtg
     4501 cactgctcgg tggcgccgat ccgcagcctc ggccaggacg tcggcggcgt cgtgctggtg
     4561 ctgcgcgccg ccggcagcga cgctcccggc gaggtgcagg ccgggcagcc cagcgagccg
     4621 tccgaggtgc tcgacagctt cggccgcgat gcgctgaccg gcctgccgga tcgccccggc
     4681 ttcgtcgcga agctcgacaa cgccctcgaa gccgtgcagg ccggcggcgc gccgcgcacg
     4741 ctgatctacc tgacgctgcg ccagatgcac agcctgaccg cgctcggcgg cgaggcgatg
     4801 gccgatcgcg tgcgggccgg ggccgcgcag gcgctgatcg acgtgctcgg cccgcgcgac
     4861 gtggccggtc gcgtcggcgc ggatgagttc gcgctgctgc tcgaaggctg gaacgccgag
     4921 accctgcccg agcagctcgc cgagatccgc gccgcgctcg gccagtactc gatggaggtg
     4981 caggggcgca cgcaccgcgt cgatgccgac atcggcgcga tcccggtcaa cgccgacagc
     5041 ggcgacagcc tcgatgtcat gtcggccgcc gatctggcct gccaccgcgc gcgggtcgcg
     5101 gcccgcagcg gcggctgagc gggcctcagc ggcgcaccgg cagcagcagc ccgagcgcga
     5161 acagcagcgc cgcgcagacc acgacgctcg ggccggccgg cgtgtcccag gcgagcgaac
     5221 cggcgagccc gccgctcacc gccagcgccc cgcagaggct cgccccgagc gccatgccct
     5281 cggggctgcg cgcccagcgt cgggccgcgg ccggcgggat gatcagcagc gaggtgatca
     5341 gcagcacgcc gaccaccttc atcgccaccg cgaccaccag cgcgatcagc agcaggaaca
     5401 ccagccgcac cgcggcgacc ggcacgccct cgacgcgggc gagttcctcg tgcacggtca
     5461 tcgccagcag cggccgccag atcagcgcca gcacgccgag cgcgagtgcg ccgccggccc
     5521 atatccacgc cagctccccc ggcgcgatcg cgaggatgtc gccgaacagc caggcgttga
     5581 gatcgatgcg cacctgctcc agaaacgccg ccgccaccag cccgagcgac agcgccgtgt
     5641 gcgcgaggat gccgagcacg gtgtcgcccg gcaggcgctt ctgctgctgc agcgcgagca
     5701 gcagcaaggc cagcagcacg ccggtcagca gcacgccgaa gccttcgccg atgccggcca
     5761 gcacgccgag cacgacgccg agcaggccgg catgcgcgag cgtgtcgccg aagtaggcca
     5821 tgcgccgcca gaccacgaag ctgccgagcg gcccggccgc gagcgccacg cccagaccgc
     5881 cggccagcgc ccgccacagg aagtcatcga gtccgaacgc gctcacgacg gcgcctcgct
     5941 gcgcgccgct gcgggggctg cgggcgttgc gggaaccggc gatgcggcgg gcccgtgcgg
     6001 gtgcggatcg ccgtgcaggt cgtgcgtgtg gtcgtggtga tgg
//
LOCUS       DAOQIE010000248         6004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7239_length_6004_cov_7.644646, whole genome shotgun sequence.
ACCESSION   DAOQIE010000248 DAOQIE010000000
VERSION     DAOQIE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7239_length_6004_cov_7.644646"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..744)
                     /locus_tag="PK144_14060"
     CDS             complement(10..744)
                     /locus_tag="PK144_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNF66899.1"
                     /translation="MPLVELDHIEKWYGQNHVLKGVTLNVERGAVVSLIGRSGSGKST
                     LLRCINGLETFDAGRITVDGEVLDPKKTDLRRFRQHVGMVFQQFNLFPHLTVGRNIML
                     APTVVKKLPAKEAEAIAREMLGRVGLSDKFDAYPEQLSGGQQQRVAIARSLAMQPSVL
                     LCDEITSALDPELVGEVLKVLERLAADGMTLILVTHEMRFARDVGSHLVFMHQGRVWE
                     EGDPKTLMANPHTPELAQFIGAVAAA"
     gene            complement(725..1381)
                     /locus_tag="PK144_14065"
     CDS             complement(725..1381)
                     /locus_tag="PK144_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNF66900.1"
                     /translation="MPEFTLWDIARNLLLAARWTVLLSLIAFAGGAVTGLLLTLARVS
                     KYPLLRLLARGYIELFQGTPLLMQLFLAFFGLSLAGIEVSPWVAASLALTLWTSAFLA
                     EIWRGCVEAIPKGQGEASASLGLSWLEAMRYVILPQALRIAIPPTVGFSVQVIKGTAL
                     ASIIGFIELTKAGTMLNNATFQPFTVFSLVALMYFALCYPLSLYAHSLEAKLHAARRT
                     "
     gene            complement(1394..2056)
                     /locus_tag="PK144_14070"
     CDS             complement(1394..2056)
                     /locus_tag="PK144_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNF66901.1"
                     /translation="MSYQFQFQELLPYTDQFVRGVWLTVQLTAVSTVLGLALGTAGAA
                     ARRYGNRLLRTLVGAYVEIVRNTPFIVQLFFIFFGLPNLGVRLTAGEAAVIAMVVNLG
                     AYSTEIIRAGLQAIPKGQVEAGQSLGMSGVQIFRYVIIRPALGKIWPALSSQCVIVML
                     GSSVVSQISAEELTFAANFIQSRNFRSFEVYAVTTLLYLALALGLRQVFGLIGRQLFA
                     RR"
     gene            complement(2070..2849)
                     /locus_tag="PK144_14075"
     CDS             complement(2070..2849)
                     /locus_tag="PK144_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019845236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNF66902.1"
                     /translation="MKLLKTLLAAVCLSLTAATAHADALTDIQGAGVLKVAVPQDFPP
                     FGSVGLDLQPQGYDIDVARLLAKGLGVKVELVPVTSANRIPYLQTKKVDLVISSLGKN
                     PEREKTIDFSIAYAPFFLAVFGPQDAKITSADALAGKTVGVTRGSVEDLELSKIAPPT
                     ADLKRFEDNNTTISAYLSGQVGLIATGNVVIAAIAAKNPPRLPVEQFKLKNSPCFIGL
                     NKNEPALQAKVNELIEAAKKDGSLDAISKKWLKMPLPDLGA"
     gene            3156..3860
                     /locus_tag="PK144_14080"
     CDS             3156..3860
                     /locus_tag="PK144_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNF66903.1"
                     /translation="MNSHRGISQQAAEAIRTLILEGGYAEGALLPSQRDLAERLGVSR
                     TSLREALSGLASRGEVRVQAGKGVFVGKGQDARGAVPAAAVDWAFGAQASPGEIFQMR
                     YGLEGYAAGLAVARTTPAVLDALAANVEALRVELRAGDFEAANRLDFDFHRRLIELSG
                     NRVLLDLLGRSAEPFIESQKLPFVRRERVMETHAEHVRILRALERQSASAATRTMQEH
                     IRRAAARIGVQFATPA"
     assembly_gap    3864..3963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3964..4438)
                     /locus_tag="PK144_14085"
     CDS             complement(<3964..4438)
                     /locus_tag="PK144_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNF66904.1"
                     /translation="MQQQIRDAGTADLDGILAIYNDAVQHTTAIWNDTPVDRTNRAAW
                     LAARTAAGYPVLVAAAADGAVLGYASFGDWRAFDGYRHTVEHSVYVHRDARGRGTGHA
                     LLAALTGRARALGKHVMVAAIDADNLASLALHRRLGFTETGRMPQVGCKFGRWLDL"
     regulatory      4529..4623
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4686..>6004
                     /gene="thiC"
                     /locus_tag="PK144_14090"
     CDS             4686..>6004
                     /gene="thiC"
                     /locus_tag="PK144_14090"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNF66905.1"
                     /translation="MSAIPEDFLRATAELSAAVTRPFPKSRKVYVEGSRPDLRVPLRE
                     IEQHPTQAGFGAEVNPPVPVYDCSGPYTDPQARIDLLAGLPSVRGGWIAERKDTEQLD
                     GPSSDYGRERAADARLAAFRFEHIRAPRRAKAGANVTQMHYARKGIVTPEMEFVALRE
                     SLRLQELRTDPRYGKLLRQHRGHSFGAAIPEMITPEFVRSEVARGRAIIPANINHPEL
                     EPMIIGRNFLVKINGNLGNSAVTSSIAEEVEKMVWGIRWGADTIMDLSTGRNIHETRE
                     WIIRNAPVPIGTVPIYQALEKVDGKAEELTWDIFRDTLIEQAEQGVDYFTIHAGVRLR
                     YVPLTADRVTGIVSRGGSIMAKWCLAHHQENFLYTHFEEICEIMKAYDVSFSLGDGLR
                     PGSLADANDAAQFGELETLGELTQVAWQHDVQVMIEGPGHVPMQLIK"
BASE COUNT         1010 a         1967 c         2015 g          912 t
ORIGIN      
        1 atcaaccctt tacgccgccg ccaccgcccc gatgaactgc gccagttccg gtgtatgcgg
       61 attcgccatc agcgtcttcg ggtcgccttc ctcccagacg cggccctgat gcatgaacac
      121 cagatggctg ccgacatcgc gtgcaaagcg catctcgtgc gtgaccagga tcagcgtcat
      181 gccgtcggcg gccaggcgtt cgagcacctt cagcacctcg ccgaccagtt ccgggtcgag
      241 ggccgaggtg atctcgtcgc agagcagcac cgagggctgc atcgccagcg agcgggcgat
      301 cgcgacgcgc tgctgctggc cgccggagag ctgctcgggg taggcgtcga acttgtccga
      361 caggccgacg cggccgagca tctcgcgggc gatggcttcg gcttccttcg ccggcagctt
      421 cttcacgacc gtcggcgcca gcatgatgtt gcgcccgacc gtcaggtgcg ggaacaggtt
      481 gaactgctgg aacaccatgc cgacgtgctg gcggaagcgg cgcaggtcgg tcttcttcgg
      541 gtccagcact tcaccatcga cggtgatgcg cccggcatcg aaggtctcca gcccgttgat
      601 gcaccggagc agcgtgctct tgcccgaacc gctgcgcccg atcagcgaca cgacggcgcc
      661 gcgctcgacg ttcagcgtca cgcccttcag cacgtggttc tggccgtacc atttttcgat
      721 gtgatcaagt tctacgagcg gcatggagct tcgcctccag ggagtgggcg tacagcgaca
      781 gcgggtagca cagcgcgaag tacatcagcg ccacgagcga gaacacggtg aagggctgga
      841 aggtggcgtt gttcagcatc gtgccggcct tggtcagctc gatgaagccg atgatcgatg
      901 cgagcgccgt gcccttgatc acctgcaccg agaagccgac ggtcggcggg atcgcgatgc
      961 gcagcgcctg cggcaggatc acgtagcgca tcgcctccag ccacgacagg ccgagactgg
     1021 ccgaagcctc gccctggccc ttcgggatcg cctcgacgca gccgcgccag atctcggcga
     1081 ggaaggcgct ggtccagagc gtcagcgcca gcgacgcggc gacccacggc gatacctcga
     1141 tgccggcgag cgacaggccg aagaaggcga gaaagagctg catcagcagc ggcgtgccct
     1201 ggaaaagctc gatgtagccg cgcgccagca gccgcagcag cgggtatttc gatacccgcg
     1261 ccagcgtcag cagcaggccg gtgacggcgc caccggcaaa ggcgatcagc gacagcagca
     1321 cggtccagcg ggccgcgagc agcaggttgc gggcgatgtc ccacagcgtg aattccggca
     1381 tggcgggcct ccgtcagcga cgcgcgaaca gttgccggcc gatcaggccg aacacctgac
     1441 gcaggccgag cgcgagcgcg aggtacagca gcgtggtcac cgcgtacacc tcgaagctgc
     1501 ggaaattgcg cgactggatg aagttggccg cgaaggtcag ttcctcggcc gagatctgcg
     1561 acaccaccga cgagccgagc atcacgatca cgcactggct cgacagcgcc ggccagatct
     1621 tcccgagcgc cgggcggatg atgacgtagc ggaagatctg cacgccgctc atgccgaggc
     1681 tctgtccggc ctccacctgg cccttcggga tcgcctgcag gccggcgcgg atgatctcgg
     1741 tcgagtacgc cccgagattc acgaccatcg cgatcaccgc cgcttcaccg gccgtcaggc
     1801 gcacgccgag gttcggcagc ccgaagaaga tgaagaacag ctgcacgatg aacggcgtgt
     1861 tgcgcacgat ctcgacgtag gcgccgacga gcgtgcgcag cagccggttg ccgtagcgcc
     1921 gggccgcggc gccggcggtg ccgagcgcga ggccgagcac cgtcgagacc gccgtcagct
     1981 gcacggtcag ccagaccccg cgcacgaact ggtcggtgta gggcagcagc tcctggaact
     2041 ggaactggta gctcatcgcg gcgtccgcct caggcaccga gatccggcag cggcatcttc
     2101 agccacttct tcgagattgc atcgagcgag ccgtccttct tcgcggcctc gatcagctcg
     2161 ttgaccttcg cctgcagcgc cggctcgttc ttgttcaggc cgatgaagca cggcgagttc
     2221 ttgagcttga actgctcgac cggcaggcgc ggcgggttct tcgcggcgat cgcggcgatg
     2281 acgacgttgc cggtcgcgat cagcccgacc tgaccggaca ggtaggccga gatcgtcgtg
     2341 ttgttgtcct cgaagcgctt caggtcggcc gtcggcggcg cgatcttgct cagttccagg
     2401 tcctcgaccg agccgcgggt gacgccgacc gtcttgccgg cgagggcgtc ggccgaggtg
     2461 atcttcgcgt cctgcgggcc gaacacggcg aggaagaacg gcgcgtaggc gatcgagaag
     2521 tcgatggtct tctcgcgctc cgggttcttg ccgaggctgg agatgaccag atcgaccttc
     2581 ttcgtctgca ggtacgggat gcggttggcg ctggtgaccg gcaccagctc gaccttgacg
     2641 ccgaggccct tcgccagcag ccgcgcgacg tcgatgtcgt aaccctgcgg ctgcaggtcg
     2701 aggccgaccg agccgaaggg cgggaagtcc tgcggcacgg cgaccttcag cacgccggcg
     2761 ccctggatgt cggtgagggc atcggcatgc gcggtggcgg cggtcagcga caggcagacg
     2821 gcggcgagca gggttttcag cagcttcatc ggacggactc cggcaggtgg acgggaaggg
     2881 gcgaaacggg ttcggcatct gcatatcgca accgccgtgc cagcatgagc gcatcaccgg
     2941 caaagccgca tggcgcctca actgttcata ccagtctgaa cagttgagcg gtgctgcaga
     3001 gtcaaaaaac cgaaatttct gcaccagatg tgtgcccgaa aaccgtgcaa tggtgcaagc
     3061 ggaaaatcgc catgaattca ggatttgcac ggtttccacg tcggcagtac gctcggtccg
     3121 gctcaggaat ccggtatcgg aacgatcaca caggtatgaa cagtcatcgc ggcatttccc
     3181 agcaggctgc cgaagcgatc cgcacgctga tcctcgaagg cggctacgcc gaaggtgcgc
     3241 tgctgccttc acagcgcgac ctggccgagc gcctcggcgt cagccgcacc tcgctgcgcg
     3301 aggcgctctc ggggctggcc tcgcgcggcg aggtgcgcgt gcaggccggc aagggcgtgt
     3361 tcgtcggcaa gggacaggat gcgcgcggcg cggtgccggc ggccgccgtg gactgggcct
     3421 tcggggcgca ggcgagcccc ggggaaatct tccagatgcg ctacgggctg gagggctacg
     3481 ccgccgggct cgccgtcgcg cgcacgacgc cggccgtgct cgatgcgctg gcggcgaacg
     3541 tcgaggcgct gcgcgtcgaa ctgcgcgccg gcgacttcga ggccgccaac cgcctcgact
     3601 tcgacttcca ccgccggctg atcgagctga gcggcaaccg tgtgctgctc gacctgctcg
     3661 gacgctcggc cgagccgttc atcgaaagcc agaagctgcc gttcgtgcgt cgcgagcggg
     3721 tcatggaaac ccatgccgag catgtgcgca tcctgcgcgc gctggagcgc cagtcggcga
     3781 gcgcggcaac gcgcacgatg caggaacaca tccgccgcgc cgccgcacgc atcggcgtgc
     3841 agttcgcgac gccggcgtga gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnccagatc cagccagcgc ccgaacttgc agccgacctg cggcatgcgg ccggtctcgg
     4021 tgaagccgag ccgccggtgc agcgccagcg aggcgaggtt gtcggcgtcg atggcggcga
     4081 ccatcacgtg cttgccgagc gcccgggcgc gtccggtcag cgctgcgagc agggcgtgcc
     4141 cggtgccgcg gccgcgggcg tcgcggtgca cgtagaccga atgctcgacc gtgtgccggt
     4201 agccgtcgaa ggcgcgccag tcgccgaagg atgcgtaacc gagcacggcg ccgtcggcag
     4261 cggccgcgac cagcaccgga tagccggccg ccgtgcgcgc cgcgagccag gcggcgcggt
     4321 tggtgcgatc gacgggcgtg tcgttccaga tcgccgtcgt gtgctgcacg gcgtcgttgt
     4381 agatggcgag gatgccgtcg aggtcggccg tgccggcatc gcgaatctgc tgctgcatgt
     4441 gcggttcttc tcccgaatgg gtccgtgcgg cggcacaccg gaaggacggc gcgcgcaatc
     4501 cgcgtgacac tgtgctgtaa tcgcggccgg atcgaagcgg gggtgctccg gattcggagc
     4561 tgagaaatac ccttggaacc tgaaccggtt agcaccggcg gagggagctt cggcgcccgg
     4621 tcgccgtccg ccccccgttt cgtcctctgc acctgccgcc cgccgataga agaggacgca
     4681 cgcccatgag cgccattccc gaggatttcc tgcgcgcgac cgccgagctg tcggccgccg
     4741 tcacgcgccc gttcccgaag tcgcgcaagg tctacgtcga aggctcgcgc cccgatctgc
     4801 gcgtgccgct gcgcgagatc gagcagcacc cgacgcaggc cgggttcggc gccgaggtca
     4861 atccgccggt gccggtctac gactgctccg gcccttacac cgatccgcag gcgcgcatcg
     4921 acctgctcgc cggcctgccc tcggtgcgcg gcggctggat cgccgagcgc aaggataccg
     4981 aacagctcga cggtccgagt tccgattacg gccgcgagcg tgccgccgac gcgcggctcg
     5041 ccgccttccg cttcgagcac atccgcgccc cgcgccgcgc gaaggccggc gccaacgtca
     5101 cgcagatgca ttacgcgcgc aagggcatcg tgacgccgga aatggaattc gtcgcactgc
     5161 gcgaaagcct gcgcctgcag gagctgcgca ctgatccgcg ttacggcaag cttctgcgcc
     5221 agcaccgcgg ccattccttc ggggccgcga ttccggaaat gatcacgccg gagttcgtgc
     5281 gcagcgaggt cgcgcgcggc cgcgccatca ttccggccaa catcaatcat ccggaactgg
     5341 agccgatgat catcggccgc aacttcctcg tgaagatcaa cggcaacctc ggcaattcgg
     5401 cggtcacgtc gagcatcgcc gaggaagtcg agaagatggt ctggggcatc cgctggggtg
     5461 ccgacaccat catggatctg tcgaccggca ggaacatcca cgaaacccgc gaatggatca
     5521 tccgcaacgc gccggtgccg atcggcacgg tgccgatcta tcaggcgctg gaaaaggtcg
     5581 acggcaaggc cgaggaactg acctgggaca tcttccgcga cacgctgatc gagcaggcgg
     5641 agcagggcgt cgattacttc acgatccacg ccggcgtgcg cctgcgttac gtgccgctga
     5701 ccgccgatcg cgtcaccggc atcgtttcgc gcggcggttc gatcatggcc aagtggtgtc
     5761 tcgcgcatca tcaggagaat ttcctctaca cgcacttcga ggaaatctgc gagatcatga
     5821 aggcctacga cgtgagcttc tcgctcggcg acggcctgcg tccgggttcg ctcgccgatg
     5881 ccaatgacgc cgcgcagttc ggtgagctgg aaacgctcgg cgaactgacg caggtcgcct
     5941 ggcagcacga cgtgcaggtc atgatcgaag gccccggcca cgtgccgatg cagctgatca
     6001 aaga
//
LOCUS       DAOQIE010000249         5988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7277_length_5988_cov_6.897017, whole genome shotgun sequence.
ACCESSION   DAOQIE010000249 DAOQIE010000000
VERSION     DAOQIE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7277_length_5988_cov_6.897017"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..848)
                     /locus_tag="PK144_14095"
     CDS             complement(381..848)
                     /locus_tag="PK144_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HNF66906.1"
                     /translation="MNALYRHDAPDGSDPLAACPMHRPVRSALARHALTALGFGAFGL
                     GTLGLFVPLLPSFVFWLLAAWAWAKARPGLTARLLAHPRLGPALYAYLCHGAIARAAK
                     RAAIGGMTLSLGLSALLLSPPPALIAVLAGGWALGCLWIATRREGPDSAAVHA"
     gene            1032..1703
                     /locus_tag="PK144_14100"
     CDS             1032..1703
                     /locus_tag="PK144_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66907.1"
                     /translation="MRLLLVEDDRMIGRSVEQGLKLEGYAVDWVLDGLSAEAALAETD
                     YELVLLDLGLPRKDGLAVLTSLRKAGNAVPVLILTARDAVSDRVRGLDAGADDYLVKP
                     FELDELAARIRALARRRGGRPTPLIEHGALTLDPASHEVTLAGTPLALSPREFALLHA
                     LLAEPGVPQSRAKLEEQVYGWGEELESNAVEVHIHALRRKLGAAWIANLRGVGWFVPR
                     EPPPA"
     gene            1735..3039
                     /locus_tag="PK144_14105"
     CDS             1735..3039
                     /locus_tag="PK144_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66908.1"
                     /translation="MNSIRRHLLLALLCAVLLGSLAGAWATWRGVREELDEIFDYNLR
                     QLALSMRDMAYGVATALPLPDPEFDFVIQVWNAAGARLYYAHRHADPPGPAGPGFSTT
                     NTSEGRWRVFAIVADGLFVQVAHPLAVRERLAAEAALRTLLPYLLLLPLLAIVVGWLV
                     GRGLAPLRRVTRAVAARRADSLEPVALADVPDEIQPLVEALNGLLARLATALQAQRDF
                     TADAAHELRSPLTALSLQVQLLERAHDATGRAAALAELRGGIARAVHLVDQLLKLARL
                     GPEVGSATEPVRLAELAARVLAEHAPLATAKAIDLGLGEVDPALSVRGDPAGLRLLLS
                     NLVGNAVRYTPDGGRVDVSVLAEGPQALLVVSDTGPGIPADERGRVFDRFYRRPGETA
                     EGSGLGLAIVRAVADRHGADVRLGDADGGGLRVEVRFPPAAA"
     gene            3090..5099
                     /gene="rep"
                     /locus_tag="PK144_14110"
     CDS             3090..5099
                     /gene="rep"
                     /locus_tag="PK144_14110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HNF66909.1"
                     /translation="MHTLNPPQRAAVLHIDGPLLVLAGAGSGKTRVITQKIAYLIRDC
                     GIRARHIAAVTFTNKAAREMQERVGKLVKGPEAQGLRVSTFHTLGLDILRRELKGAGY
                     RSGFSIYDARDTLQLLQALDDGLDKDDAQQAQWTISHWKNALVEPGDALAEAQDEATA
                     DAARLYVRYCEQLRAYNAVDFDDLILQPLRLFQREPEALERWQQRIHYLLVDEYQDTN
                     TCQYQLVRALLGVRANLTAVGDDDQSIYAWRGAQPQNLRLLAEDFPQLKIVKLEQNYR
                     SSRRILHAANTLIANNPHVFDKKLWSEHAHGEPIRVIVCKDGDDEVARVVSELVHQRL
                     RRRTQWRDYAILYRGNHQARPFETALRQQNVPYRITGGQSFFAQAEVKDVLAYLRLLV
                     NPEDDAAFVRIANVPRRELGPATLEKLAHYAAGRGLALLPACRELGLAQQLPERAAER
                     LAGFAHWLDDVAERARSGEPAAVVREMLAELHYEDWLKEQANDPKQGARRMQSVHELT
                     DWLARMGETGDGSAPPSLAERVAKMALIDLLERQEGEEAHDEVTLMTLHAAKGLEFPH
                     VFVVGIEEELLPHRNSIDAGTIEEERRLAYVGITRAQKTLTLTLAQRRRKYGNWESCE
                     PSRFLAELPQADLEWEGAGIELAPEVKKERGRERLAGLRALLDKP"
     gene            5513..>5988
                     /locus_tag="PK144_14115"
     CDS             5513..>5988
                     /locus_tag="PK144_14115"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNF66910.1"
                     /translation="METLSVSHEIHCSADRFWTTFFDPDFNEQLFVQELGFRDYCVLE
                     QKESENGIECRSTGVIESHLATPLRTLTGQEMRYVQQGCFDRSSRCYRWQSVPARMAD
                     RLRLEGSMRIEPIGTDCVRRIVEFNVEARIFGIGGLLESKVVKELSAECEVSAAAM"
BASE COUNT          911 a         2181 c         2104 g          792 t
ORIGIN      
        1 ccggcgggac ggccggcagc actgcttccg ggggcgcggc cggctccgtc accggcgccg
       61 ggggcggcgc ttccgcgggc tcggctcgcg acggcaccgg agcaggcccg gcggtgacgg
      121 gggggcgctt caggacacgc agagcgctgg gcaaggcggc ggtctccggc cgggcgccga
      181 caggcagcgc ggtgcgcgca gagtaggccg ggccgccggc agccgacagg gctgcgccgg
      241 gagtggatca ggaaaggcgg ccggagcgcg acgcagccgg cgggtccggt cgcggccgtg
      301 ccggacccgg ggcaggagaa accggacccg ccggcgctgc gtcgcgctcc agtcgttcgg
      361 tgggcggcgg tgcgcgggct tcaggcgtgc accgccgcgg aatcggggcc ttcgcgacgg
      421 gtcgcgatcc acagacagcc gagtgcccag ccgccggcga gcaccgcgat cagcgccggc
      481 ggcggcgaca gcagcagcgc cgacaggccg aggctcagcg tcatgccgcc gatcgcggcg
      541 cgcttggccg cacgggcgat ggcgccgtgg cacaggtacg catacagcgc cggcccgagc
      601 cgcggatgcg cgagcaggcg cgccgtcagc cccgggcgcg ccttcgccca ggcccaggcc
      661 gcgagcagcc agaacacgaa gctcggcagc agcggcacga acaggccgag cgtgccgagc
      721 ccgaacgcgc cgaagccgag cgcggtcagc gcgtggcgcg ccagcgccga gcgcaccggc
      781 cggtgcatcg ggcaggccgc gagcggatcg gagccatcgg gtgcatcgtg gcggtacagg
      841 gcgttcatcg gacactccgg cggatcaggg gaccgttgcc ggcgcagtct ggtccggggc
      901 ccgggccggc ggaaattgaa tgttccgctc gctccgatag ccgcctgcta tgtcagtccg
      961 caggctaccg tacccgccgg cgggccgatc agggcaaaat gcccgctttc cgccactgcc
     1021 ggagcccgcc gatgcgtctg ctgctcgtcg aggacgaccg catgatcggg cgcagcgtcg
     1081 agcagggcct gaagctggag ggctacgccg tcgactgggt gctcgatggc ctctccgccg
     1141 aagccgcgct cgccgagacc gattacgaac tggtgctgct cgatctcggc ctgccgcgca
     1201 aggacgggct ggccgtgctc acgagcctgc gcaaggccgg caacgccgtg ccggtgctga
     1261 tcctgaccgc gcgcgatgcc gtgtcggacc gcgtgcgcgg gctcgacgcc ggcgccgacg
     1321 actatctggt caagcccttc gagctcgacg aactcgccgc gcgcatccgc gcgctggcgc
     1381 ggcggcgcgg cggccggccg acgccgctga tcgagcacgg cgcgctgacg ctcgatccgg
     1441 ccagccacga ggtcacgctc gccggcacgc cgctcgcgct gtcgccgcgc gagttcgcgc
     1501 tgctgcacgc gctgctcgcc gaacccggcg tgccgcagtc gcgcgccaag ctcgaagagc
     1561 aggtctacgg ctggggcgag gagctcgaaa gcaacgccgt cgaggtccac atccacgcgc
     1621 tgcgccgcaa gctcggggcc gcctggatcg ccaacctgcg cggcgtcggc tggttcgtgc
     1681 cgcgcgagcc gccaccggca tgaagccccg ccgcgccgcc gcgcccgcgg cccgatgaac
     1741 tcgatccgcc ggcatctgct gctcgcgctg ctgtgcgccg tgctgctcgg ctcgctggcc
     1801 ggggcctggg cgacctggcg cggcgtgcgc gaggagctcg acgagatctt cgactacaac
     1861 ctgcgccagc tcgcgctgtc gatgcgcgac atggcctatg gcgtggccac cgcgctgccg
     1921 ctgcccgatc ccgagttcga tttcgtgatc caggtctgga acgccgccgg cgcccgcctg
     1981 tactacgcgc accgccacgc cgacccgccc ggcccggccg gaccgggctt ctcgaccacg
     2041 aacaccagcg aggggcgctg gcgcgtgttc gccatcgtcg ccgatgggct gttcgtgcag
     2101 gtcgcgcatc cgctggcggt gcgcgagcgg ctggccgccg aggcggcgct gcgcacgctg
     2161 ctgccctacc tgctgctgct gccgctgctc gccatcgtgg tcggctggct ggtcgggcgc
     2221 gggctcgcac cgctgcggcg cgtgacgcgc gcggtcgcgg cacggcgcgc cgacagcctg
     2281 gagccggtcg cgctcgccga tgtgccggac gagatccagc cgctggtcga ggcgctgaac
     2341 ggcctgctgg cgcggctggc cacggcgctg caggcgcagc gcgacttcac ggccgatgcc
     2401 gcgcacgaac tgcgcagccc gctgaccgcg ctgagcctgc aggtgcagct gctcgaacgc
     2461 gcccacgacg cgaccggtcg cgccgcggcg ctcgccgagc tgcgcggcgg catcgcgcgc
     2521 gccgtgcatc tggtcgatca gctgctgaag ctcgcgcgcc tcggccccga agtcggcagc
     2581 gccaccgagc cggtccgcct cgccgagctg gccgcacgcg tgctcgccga gcacgcaccg
     2641 ctcgccacgg ccaaggccat cgatctcggc ctcggcgagg tcgacccggc gctcagcgtg
     2701 cgcggcgatc cggccgggct gcggctgctg ctgtcgaacc tcgtcggcaa cgccgtgcgc
     2761 tacacgccgg acggcggccg cgtcgatgtc tcggtgctcg ccgagggtcc gcaggccttg
     2821 ctggtggtca gcgacaccgg ccccggcatc ccggccgacg agcgcggccg cgtgttcgac
     2881 cgcttctacc gccggcccgg cgaaaccgcc gaaggcagcg gcctcgggct cgcgatcgtg
     2941 cgcgcggtcg ccgatcgcca cggcgccgac gtgcgcctcg gggacgccga cggcggcggc
     3001 ctgcgcgtcg aggtgcgctt cccgccggcc gcggcctgag cctgccgccg cgctacactg
     3061 cccgccccgc cgccgcttcg tccgccgccg tgcatacgct gaatccgccc caacgcgccg
     3121 ccgtgctgca catcgacggc ccgctgctcg tgctcgccgg cgccggctcc ggcaagacgc
     3181 gcgtgatcac gcagaagatc gcctacctga tccgcgactg cggcatccgc gcccgccaca
     3241 tcgccgccgt gaccttcacg aacaaggccg cgcgcgagat gcaggagcgc gtcggcaagc
     3301 tcgtgaaggg gccggaggcg cagggcctgc gcgtgtcgac cttccatacg ctcggcctcg
     3361 acatcctgcg ccgcgaactg aagggcgccg gctaccgcag cggcttctcg atctacgacg
     3421 cgcgcgacac gctgcagctg ctgcaggcgc tcgatgacgg cctcgacaag gacgacgcgc
     3481 agcaggcgca gtggacgatc tcgcactgga agaacgcgct ggtcgagccc ggggacgcgc
     3541 tcgccgaagc gcaggacgag gcgaccgccg acgccgcccg gctgtacgtg cgctactgcg
     3601 agcagctgcg cgcctacaac gccgtcgact tcgacgacct gatcctgcag ccgctgcggc
     3661 tgttccagcg cgagcccgag gcgctcgaac gctggcagca gcgcatccat tacctgctgg
     3721 tcgacgagta ccaggacacc aacacctgcc agtaccagct ggtgcgcgcg ctgctcgggg
     3781 tgcgcgccaa cctgaccgcg gtcggcgacg acgaccagtc gatctacgcc tggcgcggcg
     3841 cgcagccgca gaacctgcgc ctgctcgccg aggacttccc gcagctgaag atcgtcaagc
     3901 tcgaacagaa ctaccgttcg agccggcgca tcctgcatgc cgccaacacg ctgatcgcca
     3961 acaacccgca cgtgttcgac aagaagctct ggagcgagca cgcgcacggc gagccgatcc
     4021 gcgtcatcgt ctgcaaggac ggcgacgacg aggtcgcgcg cgtggtctcg gaactcgtgc
     4081 accagcgcct gcgccggcgc acgcagtggc gcgactacgc gatcctgtac cgcggcaacc
     4141 atcaggcccg gcccttcgag accgcgctgc gccagcagaa cgtgccgtac cggatcaccg
     4201 gcggccagag cttcttcgcg caggccgagg tcaaggacgt gctcgcctac ctgcgcctgc
     4261 tcgtgaaccc cgaggacgac gccgccttcg tgcgcatcgc caacgtgccg cggcgcgaac
     4321 tcgggccggc gacgctcgaa aagctcgcgc actacgccgc cggccgcggg ctggcgctgc
     4381 tgccggcctg ccgcgaactc ggcctcgcgc agcagctgcc cgaacgcgcc gccgaacgcc
     4441 tcgccggctt cgcgcactgg ctggacgatg tcgccgaacg cgcgcgcagc ggcgagccgg
     4501 cggccgtggt ccgcgagatg ctcgccgagc tgcactacga ggactggctg aaggaacagg
     4561 cgaacgaccc gaagcagggc gcgcggcgca tgcagagcgt gcacgaactc accgactggc
     4621 tggcgcgcat gggcgagacc ggcgacggca gcgcgccgcc ctcgctggcc gagcgcgtcg
     4681 cgaagatggc gctgatcgac ctgctggagc gtcaggaggg cgaggaggcg cacgacgagg
     4741 tgacgctgat gacgctgcac gccgccaagg ggctggagtt cccgcacgtg ttcgtcgtcg
     4801 gcatcgagga ggaactgctg ccgcaccgca acagcatcga cgccggcacc atcgaggagg
     4861 agcggcgcct cgcctacgtc ggcatcaccc gcgcgcagaa gacgctgacg ctgacgctcg
     4921 cgcagcggcg caggaagtac ggcaactggg aaagctgcga accgagccgc ttcctcgccg
     4981 aactgccgca ggccgacctc gaatgggaag gcgccggcat cgagctcgct ccggaagtga
     5041 agaaggagcg cggccgcgaa cgcctcgccg gactgcgcgc gctgctcgac aagccctgag
     5101 cgcgcaccgc gcttgccttg ctacggatga cgcggaccgg ggccaggtca tttgcctgcc
     5161 tgcggatttt tccgggcgga atcgcgcctg cttgccattg ccgtttttta aggaaagcgc
     5221 tgctttcgcg accggagttg caggccttca acagcttgca caaccgtaat gtcacaggcg
     5281 ttgactcctg aatgctggcg attaccctgc cagcatcgtt gcaagatttg acatagatca
     5341 atacagatca atgtggcgtc ccggacggcc ggcatcgcct cgttccgaaa ctccgtgcct
     5401 gatccatcaa acgcatcggc ccgcacccga tgccgtcgat gaccattgct ctttgaaccg
     5461 gcccattccc cggggcctca ttcacacccc ggcaaaagac agggagcgaa tcatggaaac
     5521 cctttcggtc agccacgaaa tccactgcag cgccgatcgt ttctggacga cgttcttcga
     5581 tccggatttc aacgaacaac tgttcgtgca ggagctcggc tttcgcgatt actgcgtgct
     5641 ggaacagaag gaatccgaaa acggcatcga atgccgttcg accggcgtca tcgaatcgca
     5701 tctggcaacg ccgctgcgca cgctgaccgg tcaggaaatg cgctacgtgc agcagggctg
     5761 cttcgaccgc agcagccgct gttaccgctg gcagagcgtg cccgcgcgca tggccgatcg
     5821 cctgcgcctg gaaggcagca tgcgcatcga gccgatcggc accgactgcg tgcgccgcat
     5881 cgtcgaattc aacgtcgagg cgcgcatctt cggcatcggt ggcctgctgg aaagcaaggt
     5941 tgtgaaggaa ttgtcggccg agtgcgaagt cagcgccgca gcgatgaa
//
LOCUS       DAOQIE010000250         5967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7319_length_5967_cov_7.090832, whole genome shotgun sequence.
ACCESSION   DAOQIE010000250 DAOQIE010000000
VERSION     DAOQIE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7319_length_5967_cov_7.090832"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK144_14120"
     CDS             complement(<1..958)
                     /locus_tag="PK144_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HNF66911.1"
                     /translation="MRILLSDAFLRSRQHRPGARVIVHDSRVTGFECVIGARTKTFRL
                     RPRAVSALLGRWPVLTADEARAKALDLLRRVAEGEAPANVRAAQVARPTLGEALTDYL
                     AAKSLKASTARKMRERLRCQCADWLLLPLDAITLKVVQERYRSIDSTGSANEVMRYLS
                     ALFNFVAARDGVALENPVARLRKLDGLRELKPKDRMVPDAKLPAWSAAVAALADRDAA
                     DTLCFLALTGARLSEALHLTWADTDLEAGSVRFRDTKNGTDHALPLGRHLQALLCARR
                     NQATSRTNPLAADAPVFGLSETRLRRAVAEVVAAIGLSWSPHD"
     gene            complement(1089..1172)
                     /locus_tag="PK144_14125"
     tRNA            complement(1089..1172)
                     /locus_tag="PK144_14125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1137..1139),aa:Leu,seq:caa)
     gene            1392..3161
                     /locus_tag="PK144_14130"
     CDS             1392..3161
                     /locus_tag="PK144_14130"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNF66912.1"
                     /translation="MSTTNQALQAWVEEVARLTRPDRIHWCDGSEAEYRGLVELMLGN
                     GDLVALNQETHPNCYLHRSSPTDVARTEHLTFICTQDREDAGPNNNWIAPDEAHAKID
                     SLFDGCMQGRTLYVVPYCMGPIDSPLSRCGVEITDSPYVVANMRIMTRMGAAALARIE
                     REGGFVKGLHSTGDLSPERRFIMHFPEELTIKSIGSGYGGNALLGKKCHALRIGSWQA
                     RSEGWLAEHMLIVGIENPQGETHYVAAAFPSACGKTNLAMLIPPATMPGWKVWTVGDD
                     IAWLHLREDGRLWAINPEAGFFGVAPGTGAKTNRNALEMLDRDTIFTNVAVTADQQPW
                     WEGKNKDQTPVIDWKGNPFDAAKGPAAHPNSRFTVSAKQCASVSPRIDDPAGVPISAI
                     VFGGRRAELAPLVFQARDWKHGVLVGASVASETTAAATGAVGVVRRDPMAMQPFCGYN
                     FADYWGHWLSFADKSDKLPKIFHVNWFRQDADGQFMWPGFGENLRVLKWILERCAGQA
                     EAVDTPIGPMPRAEDIDTASLDVSPETLEALLSIDAGRWQAELEDIRTYFAKYGEHMP
                     RGLWEEFERTRIALEKARSAETA"
     gene            3242..3628
                     /locus_tag="PK144_14135"
     CDS             3242..3628
                     /locus_tag="PK144_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNF66913.1"
                     /translation="MYLALHHTHVTAVCLSLGLFTLRGLWMLAGSPRVHAPWTRWLPP
                     LIDTVLLASAIDLTRVLGQYPFVNGWLSAKVLALVVYIGLGAVALRHGRTYRQRATAF
                     AAALLTAGYIVGAALQHSPASWLARL"
     gene            complement(3653..4510)
                     /locus_tag="PK144_14140"
     CDS             complement(3653..4510)
                     /locus_tag="PK144_14140"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNF66914.1"
                     /translation="MDTMRIAPLHAAPPARKSAGPGAVLLLSLMLAPLPAAFVLPPPA
                     VAAESLALSTAINMAGRQRMLTQRMTMAYAQIGLDVQTDEARQRLEAAVQQFESQLAT
                     LQVSATTPEVKTALVQVDALWRPFRVIVTAAPTQAGARELIVQNEALLAAAQDVVLRL
                     QAQSQTPAAHLVDLAGRQRMLSQRIGKFYMLSAWGLASPELSAEMQKAGEQFRSALDE
                     LRKAPQNTPEISADLQEVDRLWVVYERAFRLRDGQQYIPTLVAVTSEKILQQMDRITG
                     LYEKLMAAK"
     gene            complement(4615..>5967)
                     /locus_tag="PK144_14145"
     CDS             complement(4615..>5967)
                     /locus_tag="PK144_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF66915.1"
                     /translation="LLKIANSPLYAQRRKVENLRQAMMLLGLNAAMTLALSFSLVGGF
                     GASAGGLDHLRYWRRSLLTALASRTIGLQAGLGQHREELFLAGLLQDIGILVLDAAAP
                     QDYARGLDACPGHLALAQAEQAVFGADHAEVGSWMLDRWGMPEYLVLATRFSHALTAG
                     ALADSGENARFFACTTLAGWLAEVWLNPPDEAGTARIGMLAQDLLGLDAAAVTALVDE
                     IGAQIPETSALFEIELPDAAQVTAVLDHAREVLMVRNLQIVQEAAQARRQAEHMQERA
                     RLLEERSRRDPLTGAYNRSSLQETLASEFRLATEGNWPLTLAFIDLDHFKRVNDTFGH
                     QAGDEVLCEIVRRLNAHIRCTDIVARFGGEEFVLVLPGLRRNAALTMLERVRAAIAAE
                     PCRLAEDQQVTVTLSIGIATHGEDKPYESAESLLRNADRAVYDAKRNGRNRVECYRDD
                     "
BASE COUNT          936 a         2054 c         2049 g          928 t
ORIGIN      
        1 aatcgtgcgg gctccacgac aggccgatgg ctgcaacgac ttcggcaacg gcacgacgca
       61 accgggtttc gctcaggccg aataccggtg catcggcggc aagcggattc gtgcggctgg
      121 tggcctgatt ccggcgagcg cagagcagcg cctgcaaatg ccgcccgagc ggcaacgcat
      181 ggtcggtgcc gttcttggta tcgcgaaaac gcacgctgcc ggcttccagg tccgtatcgg
      241 cccatgtcag gtgcagggct tcgctcaggc gcgcgccggt caaagccaga aagcacagcg
      301 tgtcggcagc gtcccggtcc gccagtgcgg cgaccgcggc agaccatgcc ggcagcttgg
      361 catccggcac catccggtcc ttcggcttca gttcgcgcag tccgtcgagc ttgcgcaggc
      421 gcgcgaccgg gttttccagc gccacgccat cacgggctgc cacgaagttg aacagcgccg
      481 acaggtagcg catgacttcg tttgccgacc cggtgctgtc gatgctgcga taccgctcct
      541 gcaccacctt cagcgtgatg gcgtcgagcg gcagcagcag ccagtcggcg cactgacagc
      601 gcaaccgctc gcgcatcttg cgcgcagtgc tggccttcag ggacttggcg gcgaggtagt
      661 cggtcagcgc ctcgcccagc gtcggccgcg cgacctgagc agcccgcacg ttcgccggtg
      721 cctcgccttc cgccacccgg cgcagcaggt ccagtgcttt ggcgcgggct tcatcggcgg
      781 tcagcaccgg ccagcgaccg agcagcgccg atacggcacg gggccgcagc cggaatgtct
      841 tggttcgtgc gccgatgacg cactcgaacc cggtcacgcg cgagtcatgg acaatgacac
      901 gcgcaccggg ccgatgctgg cggctgcgca ggaaggcgtc tgaaagcagg atgcgcacgg
      961 gaagtccccc ggaactgatg cacgtttgcg tcacggtttc gcgtgtcgtg catcaatttc
     1021 ggcacttggc gagctttttg cttatagctc gcaagctgcg caagctgctg atatcgaaga
     1081 tatttcgatg gtgccggagg tgggactcga acccacatgc cttgcggcgg tcgattttga
     1141 gtcgactgcg tctgccattc cgccactccg gcgagcgggg gcgcgaagta taaggaaatt
     1201 ccacggactg acaaggccgc gggtcgcgca tgccgcaacg ccttgtcttt gcacgccgta
     1261 ccgcggcgac gataaactgc gcgggcttac cggctgatga aaacgattcc tacacgagcc
     1321 cgcttcctgc cccgccgtcc gggccgcatc ggccgcctga acaccttcca cgtccgagga
     1381 gctgcatcct gatgagcacg acgaaccaag ccctgcaggc ctgggtagaa gaagttgccc
     1441 gcctgacccg tcctgaccgc atccactggt gcgatggctc ggaagccgag tatcgcgggc
     1501 tcgtggagct gatgctgggc aacggcgatc tggtcgcgct gaaccaggaa acgcacccga
     1561 actgctatct gcaccgctcc agcccgaccg acgtcgcgcg caccgagcac ctgaccttca
     1621 tctgcacgca ggatcgcgag gacgccggtc cgaacaacaa ctggatcgcg ccggacgagg
     1681 cccacgccaa gatcgactcg ctgttcgacg gctgcatgca gggccgcacg ctgtacgtcg
     1741 tgccctactg catggggccg atcgactcgc cgctctcgcg ctgcggcgtc gagatcaccg
     1801 acagcccgta cgtggtcgcc aacatgcgca tcatgacgcg catgggcgcg gcggcgctgg
     1861 cgcgcatcga gcgcgagggc ggcttcgtca agggcctgca ctcgaccggg gatctgagcc
     1921 cggaacgtcg cttcatcatg catttccccg aggaactgac gatcaagtcg atcggctcgg
     1981 gctacggcgg caacgcgctg ctcggcaaga agtgccatgc gctgcgcatc ggcagctggc
     2041 aggcgcgcag cgagggctgg ctggccgagc acatgctgat cgtcggcatc gagaacccgc
     2101 agggcgagac gcactacgtc gccgcggcct tcccgagcgc ctgcggcaag accaacctgg
     2161 cgatgctgat cccgccggcg acgatgccgg gctggaaggt ctggacggtc ggcgacgaca
     2221 tcgcctggct gcacctgcgc gaggacggcc ggctgtgggc gatcaacccc gaggccggct
     2281 tcttcggcgt cgcgcccggc accggcgcca agaccaaccg caacgcgctg gagatgctcg
     2341 accgcgacac gatcttcacg aacgtcgccg tcaccgccga ccagcagccg tggtgggaag
     2401 gcaagaacaa ggaccagacg ccggtcatcg actggaaggg caatcccttc gatgcggcca
     2461 aggggccggc ggcgcacccg aactcgcgct tcacggtgtc ggcgaagcag tgcgcgagcg
     2521 tctcgccgcg catcgatgac ccggccggtg taccgatctc ggcgatcgtg ttcggcggtc
     2581 gccgcgccga gctcgcgccg ctggtgttcc aggcgcgcga ctggaagcac ggcgtgctgg
     2641 tcggggcctc ggtggcctcc gagaccacgg cggcggcgac cggcgccgtc ggtgtcgtgc
     2701 gccgcgaccc gatggcgatg cagccgttct gcggctacaa cttcgccgat tactggggcc
     2761 actggctgtc gtttgccgac aagtccgaca agctgccgaa gatcttccac gtcaactggt
     2821 tccgccagga cgccgacggc cagttcatgt ggccgggctt cggcgagaac ctgcgcgtgc
     2881 tgaagtggat cctcgaacgc tgcgccggtc aggccgaagc cgtcgacacg ccgatcggtc
     2941 cgatgccgcg cgccgaagac atcgacaccg ccagcctcga cgtatcgccg gaaaccctcg
     3001 aagcgctgct gtcgatcgat gccggccgct ggcaggccga gctcgaggac atccgcacct
     3061 acttcgcgaa gtacggcgag cacatgccgc gcgggctctg ggaagaattc gagcgcacgc
     3121 gcatcgcgct ggagaaggcc cgcagcgccg aaacggcctg atcgccggat cgccgcccgg
     3181 acaacgccgc ccgcccgggc ggcgttttca ttggcggccc cgccagcccg aggaagcctg
     3241 catgtatctt gcgctgcatc acacccacgt gaccgcggtc tgcctgagcc tcggcctgtt
     3301 cacgctgcgc gggctgtgga tgctcgccgg ctcgccgcgc gtgcatgcgc cgtggacacg
     3361 ctggctgccg ccgctgatcg acaccgtgct gctcgcgagc gcgatcgacc tgacgcgggt
     3421 gctcggccag tatccgttcg tgaacggctg gctgagcgcg aaggtgctgg cgctcgtcgt
     3481 ctacatcgga ctcggtgccg tggcgctgcg ccacggccgc acgtaccggc agcgggcgac
     3541 ggcgttcgcg gcggcgctgc tgacggcagg ctacatcgtc ggcgccgcgc tgcagcattc
     3601 gccggcgtcg tggctggcac ggctctgagc cggcccgcca cggcccacgg gctcacttcg
     3661 cggccatcag cttttcgtac aggccggtga tgcggtccat ctgctgcagg atcttctcgc
     3721 tggtgacggc caccagcgtc gggatgtact gctggccatc gcgcagccgg aaggcgcgct
     3781 cgtagacgac ccagagccgg tcgacctcct gcaggtcggc gctgatctcg ggcgtgttct
     3841 gcggggcctt gcgcagttca tcgagcgcgc tgcgaaactg ctcgccggct ttctgcatct
     3901 ccgcgctcag ctccggcgag gccagtcccc aggcgctcag catgtagaac ttgccgatgc
     3961 gctgcgacag catgcgctgg cgaccggcga gatcgaccag atgcgcggcc ggagtctgcg
     4021 actgcgcctg cagcctgagc accacgtcct gcgcggccgc gagcagtgcc tcgttctgca
     4081 cgatcagctc gcgcgcaccg gcctgcgtcg gcgctgccgt gacgatgacc cggaacggcc
     4141 gccacagggc atcgacctgc accagggccg tcttgacctc gggagtcgtc gccgacacct
     4201 gcagcgtggc gagctgcgac tcgaactgct ggacggccgc ctccaggcgc tggcgcgcct
     4261 cgtcggtctg cacgtcgagc ccgatctggg cataggccat ggtcatgcgc tgggtcagca
     4321 tgcgctggcg accggccatg ttgatcgccg tggacagcgc cagggattcg gccgccacgg
     4381 ccggcggcgg cagcacgaag gctgcgggca gcggcgccag catcaggctc agcagcagga
     4441 cggcgccggg gccggcggat ttccgggccg ggggtgcggc atgcagcggg gcgatacgca
     4501 tcgtgtccat ggcttgcgac ctggctgatg gaggagcggt acgggaccgg gcatccggac
     4561 ggagaggccc gttagtctat cgacccgcct tccggccaac catgccgggg cgcttcagtc
     4621 gtcgcggtag cactcgacgc ggttgcggcc gttgcgcttg gcgtcgtaga cggcgcgatc
     4681 ggcgttgcgc agcaggctct cggccgattc gtacggcttg tcctcgccgt gggtggcgat
     4741 gccgatcgac agcgtcaccg tcacctgctg gtcctcggcc agccggcagg gttctgcggc
     4801 aatggcggcg cgcacgcgct ccagcatcgt cagcgcggcg ttgcggcgca ggcccggcag
     4861 caccagcacg aattcctcgc cgccgaagcg cgcgacgatg tcggtgcagc ggatgtgcgc
     4921 gttcaggcgc cggacgatct cgcagagcac ctcgtcgccg gcctgatggc cgaaggtgtc
     4981 gttgacgcgc ttgaagtgat cgagatcgat gaaggccagc gtcagcggcc agttgccttc
     5041 ggtcgcgagc cggaattcgc tcgccagcgt ctcctgcagc gagctgcggt tataggcccc
     5101 ggtcagcgga tcgcggcgcg agcgttcctc cagcaggcgg gcgcgttcct gcatgtgctc
     5161 ggcctggcgg cgcgcctggg cggcctcctg gacgatctgc aggttgcgca ccatcagcac
     5221 ctcgcgggcg tgatcgagca cggccgtcac ctgcgcggca tcgggcagct cgatctcgaa
     5281 cagcgccgag gtttccggaa tctgcgcacc gatctcatcg accagggcgg tgacggccgc
     5341 cgcatcgagt ccgagcaggt cctgcgccag catgccgatg cgcgcggtgc cggcctcgtc
     5401 gggcggattc agccagacct cggcgagcca gccggccagc gtcgtgcagg cgaagaagcg
     5461 ggcattctcg ccgctgtcgg ccagcgcccc ggcggtgagg gcatgactga agcgcgtggc
     5521 gagcaccaga tattcgggca tgccccagcg atccagcatc caggacccga cctcggcgtg
     5581 atcggcaccg aacaccgcct gctcggcctg cgccagcgcc agatgccccg ggcaggcgtc
     5641 cagccctctg gcgtagtcct gcggtgccgc ggcatcgagc acgaggatgc cgatgtcctg
     5701 cagcaggccg gccaggaaca gttcctcgcg gtgctgcccc agaccggcct gcaggccgat
     5761 cgtgcggctg gccagcgccg tcagcaggga gcgccgccag tagcgcaggt ggtcgagccc
     5821 accggccgag gccccgaagc cgccgaccag cgagaagctc agcgccagcg tcatcgcggc
     5881 gttgaggccg agcagcatca tcgcctggcg caggttctcg accttgcgcc gctgcgcgta
     5941 cagcggcgaa ttggcgatct tcagcag
//
LOCUS       DAOQIE010000251         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7355_length_5950_cov_7.824936, whole genome shotgun sequence.
ACCESSION   DAOQIE010000251 DAOQIE010000000
VERSION     DAOQIE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7355_length_5950_cov_7.824936"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK144_14150"
     CDS             complement(<1..206)
                     /locus_tag="PK144_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNF66916.1"
                     /translation="MTDKPGVQAHRQAGFTLVELMIVCVIAAIVMVWGIPSYREMVQN
                     QQRDAALFDLIADLNLARSESLKRG"
     gene            239..314
                     /locus_tag="PK144_14155"
     tRNA            239..314
                     /locus_tag="PK144_14155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Thr,seq:cgt)
     gene            complement(384..1013)
                     /locus_tag="PK144_14160"
     CDS             complement(384..1013)
                     /locus_tag="PK144_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66917.1"
                     /translation="MTPKNLRFMLALFLSGLFFAPTSNAGLLGKTLTLAYYSGTTAIS
                     GTSVQVTGGTSVELSSWPTVGFPTSGLKLTVDVTDTSIIFSNTQEVILSFFTPATPSI
                     YLVIKDATNNLPDFSSNPTDTFLAHTITGLDSSDLNVTSDRISLLLNGASMPTKTPDG
                     YITLQIGFANTTPPVNVPAPAPLVLIITGLLLSHVSKKNVFARQGKRPA"
     gene            complement(1178..3997)
                     /gene="uvrA"
                     /locus_tag="PK144_14165"
     CDS             complement(1178..3997)
                     /gene="uvrA"
                     /locus_tag="PK144_14165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF66918.1"
                     /translation="MDFIRIRGARTHNLKNVDLDLPRNRLIVITGPSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLALTDKPDVDAIEGLSPAIAIEQKTASHNPRSTVGTVT
                     EIHDHLRLLYARAGTPHCPEHGHALDAQTVAQMVDAVLARPEGERLLLLAPVVRERKG
                     EHAQLFTELRAGGFLRVRIDATVHELDALPALDGKTRHTIEVVVDRLRVRSDQRARLA
                     ESFETALKLADGLARVESLDADGEALAFSARHACPECGFTVPELEPRLFSFNSPAGAC
                     PACDGLGTQNWFDPARVVVEPGRSLADGAIRGWERRNPWSWALLQALAAHYGFDPEQP
                     FRELPAAAREVVLYGSGTTELDVQHEGERGARTQRQRFEGVIPNLERRYRETESATVR
                     EELARLQAERLCPACRGARLNPAARAVRVGGRSLPEIAALPIDACREWFLKLDLPGRH
                     GVIATPILRELRSRLGFLGDVGLDYLTLDRAAETLSGGEAQRIRLASQIGAGLVGVLY
                     VLDEPSIGLHPRDIERLLKTLMHLRDLGNTVVVVEHDEATIRAADHIVDMGPAAGIQG
                     GRIVAQGTPHAISVHPDSLTGEYLSGRRRIEPGKPRLPDGRWLTVRGATGNNLKDIDA
                     AFPVGLFTCVTGVSGSGKSTLVNDTLLRALLRHLHGSRETPAPHRAIEGLAHFDKVIA
                     IDQSPIGRTPRSNPATYTGLFGPIRELYAGVPEARARGYGPGRFSFNVKGGRCEACQG
                     DGLIRVEMHFLPDVYVPCDVCHGRRYERETLEVKYKGRSIADTLDLTIDAARPVFAAV
                     PAIARKLDTLAEVGLGYLTLGQSATTLSGGEAQRVKLAKELARRDTGRTIYILDEPTT
                     GLHFQDVAQLLAVLWRLRDDGNTVVVIEHDLDVIRCADWLIDLGPEGGKAGGQIIATG
                     SATSMTEENFGHTSCWLKQKTGVSS"
     gene            4191..5594
                     /locus_tag="PK144_14170"
     CDS             4191..5594
                     /locus_tag="PK144_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66919.1"
                     /translation="MTSRRTTTLAGEAMTAQERRVAGWLAGVFSLRMLGLFMILPVFA
                     LYAEHLDGATPALAGLAIGIYGLAQAIFQIPFGLLSDRFERKQIIIVGLVIFALGSVV
                     AALATSINGVILGRALQGMGAISAAVMALAADLTREEHRTKVMATIGVSIGVSFAVSM
                     VAGPVLNGFVGVPGIFWITALLALGGIGVVKFLVPDPFDHRLHRDAQPVPAQFLRVLR
                     DPQLLRLDAGIFALHLILTATFVAVPHALKNLIGMPASTHWELYLPTMVLGMGAMVPF
                     VIVAEKQRRLKQVFLGAIALLGVAEVALVGFHAGFWSIGIAMFLFFTAFNLLEATLPS
                     LVAKMAPPDAKGTAMGFYASTQFLGAFCGGALGGWLHGRYGLAAVFQLCALVALGWLA
                     LAATMKNPRYLSSYLLNVGAMDEAAAQRLGARLTEVRGVAEAVVSGADGVAYLKIDRH
                     ALDEPALLAFSVSRPPA"
     gene            5674..>5950
                     /gene="ssb"
                     /locus_tag="PK144_14175"
     CDS             5674..>5950
                     /gene="ssb"
                     /locus_tag="PK144_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF66920.1"
                     /translation="MARGINKVILVGNLGADPETKYMPSGDAITNLRIATSDTWTDKS
                     SGQKQERTEWHRVVMFKKLAEIAAEYLRKGSQVYIEGSLRTRKWQDKE"
BASE COUNT          990 a         1861 c         1997 g         1102 t
ORIGIN      
        1 ccgcgcttga gggactcgct gcgggcgagg ttgaggtcgg cgatcaggtc gaacagcgcg
       61 gcgtcgcgct gctggttctg caccatctcg cggtagctgg ggatgcccca gaccatgacg
      121 atcgcagcga tgacgcagac gatcatcagt tcaacgagcg tgaagccggc ctggcgatgt
      181 gcttgcactc cgggcttgtc agtcatcgtg tgttccctta ccatgcgcgc cctgtcacgc
      241 cggaatagct cagtcggtag agcaactgat tcgtaatcag taggtcggag gttcgattcc
      301 tctttccggc accagaatag caaaaagcca agcatcggaa gatgcttggc ttttttgttt
      361 gtcagcagcg atgaggattc aggtcatgct ggccgcttgc cttggcgagc aaagacgttc
      421 tttttgctga cgtgactcaa cagcagaccg gtaatgatca gcaccagcgg ggctggggcg
      481 ggtacgttaa cagggggggt ggtgttggca aaaccgattt gtaaagtgat atatccatca
      541 ggagtcttgg taggcatcga agcgccgttc aacagcagtg aaatgcggtc gctggtcacg
      601 tttaaatcgc tgctgtcgag tccggtaatt gtgtgtgcga gaaatgtatc agtgggattg
      661 gaggaaaagt caggcaaatt attggtggca tccttgatca ccagatagat ggaaggtgtt
      721 gcaggggtga agaatgacaa tatcacttcc tgggtgttgc tgaaaatgat gctggtatca
      781 gtaacgtcaa cggtgagctt caagccgctg gtgggaaaac caactgtagg ccagctggat
      841 agttcgacag acgttccgcc ggtaacctga acgctggttc ccgagattgc agtggtgccg
      901 ctataatatg caagtgtaag agtttttcct aataggccgg cattggatgt cggggcaaaa
      961 aataagccgg ataaaaacaa ggcaagcatg aaccgaaggt tttttggggt catttggtat
     1021 attgctccgg ctgtcttgaa tgcccggggt tgcttgtcaa agccgcaaaa gcagggctgt
     1081 atattaatca tgcatttttt gtgccgatgt tgttaagttt atgtataata aagtaaaaac
     1141 agcttttcat caaagccggg agagacatcg gtgtaaatta tgatgacact cctgtctttt
     1201 gtttgagcca acagcttgtg tggccgaagt tttcctcggt catgctggtg gcagaaccgg
     1261 tggcgatgat ttgtccgcca gcctttccgc cctcgggccc cagatcgatc agccagtccg
     1321 cacagcggat cacatccagg tcgtgctcga tgaccacgac ggtgttgccg tcgtcgcgca
     1381 gtcgccacag cacggccagc agttgcgcaa catcctgaaa atgcaggccg gtggtgggtt
     1441 cgtcgaggat gtatatggtg cggccggtgt cgcggcgcgc cagttccttc gccagtttca
     1501 cacgctgcgc ctcaccgccg gacagcgtag ttgcactctg gccgagcgtc aggtagccga
     1561 gcccgacctc ggccagcgtg tcgagcttgc gggcgatcgc cggtaccgcc gcgaacaccg
     1621 gccgggcggc atcgatggtc aggtccagcg tgtcggcgat gctgcggccc ttgtatttca
     1681 cctccagcgt ctcgcgctcg tagcggcgtc cgtggcagac atcgcatggc acgtaaacgt
     1741 ctggcaggaa atgcatttcg acccggatca ggccatcgcc ctgacaggct tcgcagcgcc
     1801 cgcccttgac gttgaagctg aatcgccccg gcccgtaacc gcgtgcgcgc gcctccggca
     1861 cgccggcata cagctcgcgg atcggtccga acaggccggt gtaggtggcc ggattcgagc
     1921 gcggcgtgcg tccgatcggg ctctggtcga tggcgatgac cttgtcgaag tgcgcgagtc
     1981 cctcgatcgc ccggtgcgga gccggcgttt cgcggctgcc gtgcaggtgg cgcagcagtg
     2041 cgcgcagcag cgtgtcgttg accagagttg acttgccgga accggaaacc ccggtcacgc
     2101 aggtgaacag gccgaccggg aaggccgcgt cgatgtcctt caggttgttg ccggtggcgc
     2161 cgcgcacggt cagccagcgg ccatccggca agcgcggctt acctggctcg atgcgccgtc
     2221 ggcccgacag atattccccg gtcagcgaat ccggatgcac ggaaatggca tgcggtgtgc
     2281 cttgtgcaac gatccgaccg ccctgaatgc cggctgccgg ccccatgtcc acaatatgat
     2341 cggcagcccg gatcgttgcc tcgtcatgct cgacgacgac gacggtattg ccgagatcac
     2401 gcaggtgcat cagggttttc agcaggcgtt cgatatcgcg cggatgcagg ccgatcgacg
     2461 gttcatcgag tacatacagc accccgacca gtccggcgcc gatctgcgaa gccagccgga
     2521 tgcgctgcgc ctcgccgccg gacagggttt cggcggcgcg atcgagagtc aggtaatcga
     2581 gcccgacatc accgagaaaa ccgagccggc tgcgtaattc acgcaggatt ggtgtggcaa
     2641 tcacgccatg gcggcccggc agatcaagtt tcagaaacca ttcacggcag gcatcgatcg
     2701 gcagggccgc gatttccggc aggctgcgac caccgacgcg gaccgcgcgc gccgccggat
     2761 tcagccgcgc gccccggcag gccggacaga gccgttccgc ctgcaggcgt gcgagttcct
     2821 cacgtaccgt tgccgattcg gtttcgcgat agcggcgttc gaggttgggg atgacgcctt
     2881 cgaagcgctg gcgctgcgtg cgtgcgccgc gctcgccttc atgctgcacg tcgagttcgg
     2941 tcgtgccgct gccgtacagc accacctcgc gcgccgccgc cggcagctcg cgaaacggct
     3001 gctccgggtc gaagccgtag tgcgcggcca gcgcctgcag cagcgcccag gaccaggggt
     3061 tgcgccgctc ccagccgcgg atcgcgccgt cggcgagcga gcgccccggc tcgacgacga
     3121 cgcgcgccgg gtcgaaccag ttctgcgtgc cgaggccgtc gcaggccgga caggcgccgg
     3181 ccgggctgtt gaacgagaac agccgcggtt cgagttccgg cacggtgaag ccgcattcgg
     3241 ggcaggcgtg gcgggcgctg aacgcgagcg cctcaccgtc ggcatcgagc gattcgaccc
     3301 gtgccagccc gtcggcaagc ttcagcgccg tctcgaagga ttccgccagc cgggcgcgct
     3361 ggtcgctgcg cacgcgcagg cggtcgacga cgacctcgat cgtgtgccgg gtcttgccgt
     3421 cgagggccgg cagcgcgtcg agttcatgca cggtggcgtc gatgcgcacg cgcaggaagc
     3481 cgccggcgcg cagctccgtg aacagttgcg catgctcgcc cttgcgctcg cgcaccaccg
     3541 gtgcgagcag cagcaggcgc tcgccttcgg ggcgcgcgag cacggcatcg accatctgcg
     3601 ccacggtctg cgcatcgagc gcatggccgt gctccgggca gtgcggcgtg ccggcgcgcg
     3661 cgtagagcag gcgcaggtgg tcgtggatct cggtgacggt gccgaccgtc gagcgcggat
     3721 tgtgcgaggc ggtcttctgc tcgatcgcga tcgccggcga caggccctcg atcgcatcga
     3781 catcgggctt gtcggtcagc gccaggaact gccgcgcgta ggccgacagc gattcgacgt
     3841 agcggcgctg gccctcggcg tacagcgtgt cgaaggcgag cgaggacttg ccggaacccg
     3901 acgggccggt gatgacgatc agccggttgc gcggcaggtc gagatcgacg ttcttcaggt
     3961 tgtgcgtgcg cgcaccgcgg atgcggatga agtccatgca ggatcggcct cggtgctcgg
     4021 gccgccgcgg attaagccgc ggctaagcgg gtgggggagg gcgctctgtt acactgcagc
     4081 atccggcttt tccgcattcc tgtccgattt ccgatccgag ctttcatccg tccggccggt
     4141 gtggccccgc accggtcccg gccggcgcat ggcccgaggc gaggctttcc atgacttcca
     4201 gacgcaccac gacgctggcc ggcgaggcaa tgaccgcgca ggagcgccgc gtcgccggct
     4261 ggctggccgg cgtgttctcg ctgcgcatgc tcggcctgtt catgatcctg ccggtgttcg
     4321 cgctgtatgc cgagcacctc gatggcgcca cgccggcgct cgccggcctc gcgatcggca
     4381 tctacggcct ggcgcaggcg atcttccaga ttccgttcgg cctgctgtcc gaccgcttcg
     4441 agcgcaagca gatcatcatc gtcggcctcg tgatcttcgc gctcggttcg gtcgtggcgg
     4501 cgctcgcgac cagcatcaac ggcgtgatcc tcggccgcgc gctgcagggc atgggcgcga
     4561 tctcggcggc ggtgatggcg ctcgccgccg atctgacgcg cgaggagcac cgcaccaagg
     4621 tcatggcgac gatcggcgtg tcgatcggcg tgtcgtttgc ggtgtcgatg gtcgccggac
     4681 cggtgctgaa cggcttcgtc ggcgtgcccg gcatcttctg gatcacggcg ctgctcgcgc
     4741 tcggcggcat cggcgtcgtg aagttcctcg tgcccgatcc gttcgatcat cgcctgcacc
     4801 gcgacgcgca gccggtgccg gcgcagttcc tgcgcgtgct gcgcgatccg cagttgctgc
     4861 gcctcgatgc cggcatcttc gcgctgcacc tgatcctgac cgcgaccttc gtcgccgtgc
     4921 cgcatgcgct gaagaacctg atcggcatgc cggccagcac gcactgggag ctctacctgc
     4981 cgaccatggt gctcggcatg ggggcgatgg tgccgttcgt gatcgtcgcc gagaagcagc
     5041 ggcggctgaa gcaggtgttc ctcggtgcga ttgcgctgct cggcgtcgcc gaggtggcgc
     5101 tggtcggctt ccacgccggc ttctggagca tcggcatcgc gatgttcctg ttcttcacgg
     5161 ccttcaacct gctcgaagcg acgctgccgt cgctggtcgc gaagatggcg ccgcccgatg
     5221 ccaagggcac ggcgatgggc ttctacgcca gcacgcagtt cctcggcgcc ttctgcggcg
     5281 gcgcgctcgg gggctggctg cacggacgct acggcctcgc ggcggtgttc cagctctgcg
     5341 cgctggtcgc gctcggctgg ctggcgctgg ccgcgaccat gaagaacccg cgctatctgt
     5401 cgagctacct gctgaacgtc ggggcgatgg acgaggctgc ggcccagcgc ctcggtgcgc
     5461 gcctgaccga agtccgcggc gttgccgagg ccgtggtcag cggtgccgac ggggtcgcct
     5521 atctgaagat cgaccgccac gcgctcgacg agccggcgct gctcgcgttc tcggtgtcgc
     5581 ggccgccggc ctgagccgca ctagaatccc cggcatcccg gcctcatatc agccggccgg
     5641 catcccctca ccagcaccgc ggagcgctcc ctcatggcca gaggcatcaa caaggtcatc
     5701 ctcgtcggca atctcggggc cgatcccgaa accaagtaca tgccgagcgg tgatgcgatc
     5761 accaacctgc gcatcgcgac ttcggacacc tggaccgaca agagctcggg ccagaagcag
     5821 gaacgcaccg agtggcaccg cgtggtgatg ttcaagaagc tcgccgagat cgccgccgaa
     5881 tacctgcgca agggttcgca ggtctacatc gagggcagcc tgcgcacgcg caagtggcag
     5941 gacaaggaag
//
LOCUS       DAOQIE010000252         5936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7387_length_5936_cov_7.005101, whole genome shotgun sequence.
ACCESSION   DAOQIE010000252 DAOQIE010000000
VERSION     DAOQIE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_5936_cov_7.005101"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="PK144_14180"
     CDS             <1..1974
                     /locus_tag="PK144_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNF66921.1"
                     /translation="LDDDGDDGPDAAARVAAPVTVNTGAPDPDPDDDETPPVAQAGGA
                     CPPRPVVMAADEEEEGGDGGSSPAPAPVPDAAELVIGARRNAPIEGPVTQPVAAPAPI
                     PLPAPAPVPLPAPAAAAPVAVPAAAPRPQPPARAEGVSEAAAALFGLRPKREVLDPLP
                     PLELLDLPREQAAGYSPEALDGMSRQVESKLAEFGVDVTVVNVEPGPVVTRFELDLAP
                     GVKVSQISNLAKDVARGLSVVSVRVVEVIPGKSVIGLEIPNRRRQIVSLREIIAAPVW
                     QGAGSPLTVALGHDIGGRPVVGNLAKMPHVLVAGTTGSGKSVAVNCMLISMLYKAGAE
                     QLRLILIDPKMLELSIYEGIPHLLAPVVTDMKDAANALRWCVAEMERRYRLMAALRVR
                     NIAGFNKQVSEAIELGEPIPDPLWKNEDSLDPERPLLEPLPYVVVVIDELADMMMIVG
                     KKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIALTVASKIDSRTI
                     LDQMGAEALLGHGDMLYLPPGTGFPQRVHGAFVDDHEVNAVVEYLKQTGEPDYVTGIL
                     DEPVDGAAFPGDPLAGGGGDSDGDSDPLYDQAVRIVVESRRASVSGVQRRLKIGYNRA
                     ARLVEEMERAGIVGPIQSNGSREVLVGGAQADA"
     gene            2208..2837
                     /gene="lolA"
                     /locus_tag="PK144_14185"
     CDS             2208..2837
                     /gene="lolA"
                     /locus_tag="PK144_14185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNF66922.1"
                     /translation="MSLLSRLPAGLLLACALLLSVPARAEPSVTQYFHDLQSLQAGFT
                     QRVSDAQGRIVQESAGSMAMQKPGRFRWDYTAPSPQTIVADGQRLWVHDPGLDQVTVR
                     PLDAALGGTPLALLSGAAPIEDVFKVGPVQNRGGLSWYLLTPKDDGSDFKALRVGFRG
                     DTLAALELEDALGGRTRVEFSHLQRNGRVDPAQFRFTPPPGTDVIGDAG"
     gene            2846..4168
                     /locus_tag="PK144_14190"
     CDS             2846..4168
                     /locus_tag="PK144_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNF66923.1"
                     /translation="MTTGADGADPRPLADRLRPQRLDELVGQTHLFAPGRPLRVALEA
                     GRPHSMVLWGPPGTGKTTLARLIAGHCDAHFIALSAVLAGVKEVRAAVEEAQAVRARE
                     ARATVLFVDEVHRFNKAQQDAFLPFVEDGTVTFVGATTENPSFALNNALLSRARVYVL
                     KRLEAAELGALIDRALADAQHGLGGRGLVADAPTRERLVLAADGDGRRLLGLLELAAE
                     LAEAEGRGFADAVIEVVQGGVRRFDRGGDLFYDQISALHKAVRGSAPDAALYWLARMI
                     DGGCEPLYLARRIVRMASEDIGNADPRALRLALDAAETFERLGSPEGELALAQAVVYL
                     ACAAKSNAVYTAWQGALADARSHGTLEVPEHLRNAPTKLMKSLGYGRRYRYAHDEPDA
                     YAAGETYLPDALAGRRYYDPVPRGLEIRIREKLERLRELDRLAREGRD"
     gene            4308..5117
                     /locus_tag="PK144_14195"
     CDS             4308..5117
                     /locus_tag="PK144_14195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF66924.1"
                     /translation="MLTRLLRHPLGVPAGTLAITVLAILASTTLLWLSSRLLPWWPVP
                     MSFGLKLTMLLTALVALPFAWALLSLLQRAEQLREQLQRIAAFDELTGAYTRRQFSVL
                     AQSALARLTASGGCASLLVVDADWFKAINDRHGHVGGDAVLQHLGQQLLAQFGVDALV
                     GRYGGEEFLVLLPDAGPAMACREAHALRLRVAGLRIPARDGGPIAVTVSIGVASASGT
                     TLALGALYRAADAALYRAKHEGRDRVAQAGGPLGPHCLSCTGAPACRHEDP"
     gene            5219..>5936
                     /locus_tag="PK144_14200"
     CDS             5219..>5936
                     /locus_tag="PK144_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF66925.1"
                     /translation="MTEAHAAPGLSRLRWRMYALLVGAYVLVYFHRMAPGVVAGELQQ
                     AFEASAAALGGLAAAYFYIYAAMQLPAGVLADTWGTRRTVVAGNIVAALGAVLFASAG
                     SLAQATLGRLAIGAGVSVVFVGLMKFNAVWFEGRHYGKVAGLTLLIGNLGSIAAAGPL
                     AGLLALVGWREVFTAIGGLSLVLAALTWQFVRDRPEEAGLAPLHVLPPAAARPHWSKE
                     LATVLRERRLWPVVLVQFGMI"
BASE COUNT          807 a         2110 c         2138 g          881 t
ORIGIN      
        1 ctcgacgatg acggggatga cggtccggac gcagcggcgc gggtggcagc cccggtgacg
       61 gtgaacaccg gcgcgcctga tccggatccg gacgacgacg agacaccgcc cgtggcgcag
      121 gccggcggtg catgcccgcc gcggccggtt gtgatggcgg cggacgagga ggaggagggt
      181 ggggatggcg gatcatcgcc ggctccggca ccggtgccgg acgctgccga actggtgatc
      241 ggcgcgcgcc gcaatgcgcc gatcgagggg ccggtgaccc agccggtggc tgcaccggcg
      301 ccgatcccgc tcccggcacc ggccccggtt cccctgccgg cacctgcggc cgccgcgccc
      361 gtggcggttc cggcggctgc gccgcgaccg cagccgccgg cgcgtgccga aggcgtgtcc
      421 gaggctgcgg cggcgctgtt cgggctgcgg ccgaagcgcg aggtgctcga tccgctgccg
      481 ccgctcgaac ttctggacct gccgcgcgag caggccgccg gctactcgcc ggaggcgctc
      541 gacggcatgt cgcgtcaggt cgagtccaag ctcgccgagt tcggcgtcga cgtgaccgtc
      601 gtcaacgtcg aaccggggcc ggtggtcacg cgcttcgagc tcgacctcgc ccccggcgtc
      661 aaggtcagcc agatcagcaa tctcgccaag gacgtggcgc gcgggctgtc ggtggtcagc
      721 gtgcgcgtcg tcgaggtcat ccccggcaag tcagtgatcg gactggagat tccgaaccgg
      781 cgccggcaga tcgtgtcgct gcgcgagatc atcgccgcgc cggtctggca gggagcgggt
      841 tcgccgctga cggtcgcgct cggccacgac atcggtggcc ggccggtggt cggcaacctc
      901 gccaagatgc cgcacgtgct ggtggccggc acgaccggtt ccggcaagtc ggtggcggtc
      961 aactgcatgc tgatcagcat gctgtacaag gcgggcgccg agcagctgcg cctgatcctg
     1021 atcgacccga agatgctgga gctgtcgatc tacgagggca tcccgcacct gctggcgccg
     1081 gtggtcaccg acatgaagga cgccgccaac gcgctgcgct ggtgcgtcgc cgagatggag
     1141 cgccgctacc ggctgatggc ggcgctgcgc gtgcgcaaca tcgccggctt caacaagcag
     1201 gtctccgagg ccatcgaact cggcgagccg atcccggacc cgctgtggaa gaacgaggac
     1261 agcctcgatc ccgaacggcc gctgctcgaa ccgctgccct acgtggtcgt ggtcatcgac
     1321 gagctggccg acatgatgat gatcgtcggc aagaaggtcg aagagctgat cgcgcggctg
     1381 gcgcagaagg cgcgcgccgc cggcatccac ctgatcctcg ccacgcagcg gccgtcggtc
     1441 gacgtgatca ccggcctgat caaggccaac atcccgacgc gcatcgcgct gaccgtcgcc
     1501 tcgaagatcg actcgcgcac gatcctcgac cagatgggcg ccgaagccct gctcggccac
     1561 ggcgacatgc tctacctgcc ccccggcacc ggcttcccgc agcgcgtgca cggggccttc
     1621 gtcgacgatc acgaggtcaa tgccgtcgtc gagtacctga agcagaccgg cgagcccgac
     1681 tacgtgaccg gcatcctcga tgagccggtg gacggagccg cattcccggg cgatccgctg
     1741 gccggcgggg gcggcgacag cgacggcgac agcgatccgc tgtacgacca ggccgtgcgc
     1801 atcgtcgtcg agagccgccg cgcctcggtc tccggcgtgc agcggcggct gaagatcggc
     1861 tacaaccgcg ccgcgcggct ggtcgaggag atggaacgcg ccggcatcgt cgggccgatc
     1921 cagtccaacg gctcgcgcga ggtgctggtt ggcggcgccc aggccgatgc ctgataccga
     1981 tcacacgtaa acgcataaac gcatggtttt tctccccctc tccccaaccc ctctcccacg
     2041 aggggcgagg ggctgaaaag cgaagcgtgc tgatgtgatc ggtatgagca ctccggccgc
     2101 cggtttgtcg cgatccggta acggcggcgc accggcggcc ggcgccgcgg tcgataccgc
     2161 agccgcgcgg ctgtgcggtc cgtgttccct gttcgtcgag gcctgtcatg tccctgctgt
     2221 ccagacttcc cgccggcctg ctgctcgcat gcgcgctgct gctgagcgtg ccggcgcgcg
     2281 ccgagccatc ggtcacgcag tacttccacg acctgcagtc gctgcaggcc ggcttcacgc
     2341 agcgcgtcag cgatgcgcag gggcggatcg tgcaggaatc ggccggttcg atggcgatgc
     2401 agaagcccgg gcgcttccgc tgggattaca cggcgccgag cccgcagacc atcgtcgccg
     2461 atggtcagcg cctgtgggtg catgaccccg gcctcgacca ggtcaccgtg cgcccgctcg
     2521 atgccgcgct cggcggcaca ccgctggcgc tgctgtcggg ggccgcaccg atcgaggacg
     2581 tgttcaaggt cgggccggtg cagaaccgcg gcggcctgag ctggtatctg ctgacgccga
     2641 aggacgacgg cagcgacttc aaggcgctgc gcgtcggctt tcgcggcgac acgctggccg
     2701 cgctcgaact ggaggatgcg ctcggcgggc gcacgcgcgt cgagttcagc catctgcagc
     2761 gcaacggccg cgtcgatccg gcgcagttcc gcttcacgcc gccgcccggc accgacgtga
     2821 tcggcgacgc cggctgaacc cgacgatgac gaccggcgca gacggtgcag atccgcgtcc
     2881 gctcgccgac cggctgcgcc cgcagcggct cgacgagctg gtcggccaga cgcatctgtt
     2941 cgcgccgggc cggccgctgc gcgtcgcgct cgaagccggc cggccgcatt cgatggtgct
     3001 ctggggcccg cccggcaccg gcaagaccac gctggcgcgg ctgatcgccg ggcattgcga
     3061 cgcgcacttc atcgcgctgt cggcggtgct cgccggcgtc aaggaggtcc gcgccgcggt
     3121 cgaggaggcg caggccgtgc gtgcgcgcga ggcgcgcgca acggtgctgt tcgtcgacga
     3181 ggtgcaccgc ttcaacaagg cgcagcagga tgccttcctg cccttcgtcg aggacggcac
     3241 cgtcaccttc gtcggcgcga ccaccgaaaa tccgagcttt gcgctgaaca acgcgctgct
     3301 gtcgcgggcg cgtgtctatg tgctgaagcg cctcgaagcc gccgaactcg gtgcgctgat
     3361 cgaccgcgcg ctcgccgatg cgcagcacgg cctcggcggt cgcggactgg tcgccgacgc
     3421 gccgacgcgc gagcggctgg tgctcgccgc cgacggcgat ggccggcgcc tgctcgggct
     3481 gctggagctg gcggcggagc tggccgaggc cgaaggcaga ggcttcgccg acgcggtcat
     3541 cgaagtcgtg cagggcggcg tgcgccgctt cgatcgcggc ggcgatctgt tctatgacca
     3601 gatctcggcg ctgcacaagg ccgtgcgcgg ctcggcgccg gatgcggcgc tgtactggct
     3661 ggcgcgcatg atcgacggcg gctgcgagcc gctgtatctg gcgcggcgca tcgtgcgcat
     3721 ggcgagcgag gacatcggca acgccgatcc gcgggcgctg cgcctggcgc tcgatgccgc
     3781 cgaaaccttc gagcgcctcg gcagtcccga gggcgaactc gcactcgcgc aggccgtcgt
     3841 gtatctcgcc tgcgccgcca agagcaacgc cgtctacacc gcctggcagg gcgcgctcgc
     3901 cgatgcccgc agccatggca cgctcgaagt ccccgagcac ctgcgcaacg caccgacgaa
     3961 gctgatgaaa tccctgggct acggccggcg ctaccgctac gcgcacgacg aacccgacgc
     4021 ctacgccgcc ggtgagacct atctgccgga tgcactcgcc ggccgccgct attacgatcc
     4081 ggtgccgcgc ggcctcgaga tccgcatccg cgagaagctc gaacgcctgc gcgaactcga
     4141 ccggctggcc cgcgagggtc gggactgata ccggtcacac gtaagcgcat ggtttttctc
     4201 cccctctcca caacccctct cccacgaggg gcgaggggct gaaaagcgca gcgttccggt
     4261 gtgattggca tgaaagcccg gcttctcccc gacaaggacg tttgatgatg ctgacccgac
     4321 tgctgcgcca tccgctgggc gtgcccgccg gcacgctggc gatcaccgtg ctcgcgatcc
     4381 tcgcctcgac cacgctgctg tggctgagca gccggctgct gccgtggtgg ccggtgccga
     4441 tgtcgttcgg gctgaagctg acgatgctgc tgacggcgct ggtggcgctg ccgttcgcgt
     4501 gggcgctgct gtcgctgctg cagcgtgccg agcagctgcg cgagcagctg cagcgcatcg
     4561 ccgctttcga tgagctgacc ggcgcctata cgcgccggca gttctcggtg ctcgcgcaat
     4621 cggcgctggc gcggctgacg gcgagtggtg gctgcgcctc gctgctggtc gtcgatgccg
     4681 actggttcaa ggcgatcaac gaccgccacg gccacgtcgg cggcgatgcc gtgctgcagc
     4741 acctcggtca gcaactgctc gcccagttcg gggtggacgc gctggtcggg cgctacggcg
     4801 gcgaggagtt cctggtgctg ctgccggacg ccgggccggc gatggcctgc cgcgaggcac
     4861 atgcgctgcg cctgcgcgtc gccgggctgc ggattccggc ccgcgacggc ggtcccatcg
     4921 cggtcacggt cagcatcggg gtcgccagcg cgtcgggaac gacgctggca ctcggtgcgc
     4981 tgtaccgcgc cgccgatgcc gcgctgtatc gcgccaagca cgaaggccgc gaccgggtcg
     5041 cgcaggcggg cggaccgctc ggtccgcact gcctgagctg taccggcgcg ccggcctgcc
     5101 ggcacgagga cccctgaaca ggcggagccg ggcatcgccg tgaacccggc cggcgcggcc
     5161 ggcgtgcgac actgcgcagc cccgcccggc ttgccgggcc gaccggactg tcccgctgat
     5221 gaccgaagcc cacgccgccc ccggcctgtc gcgcctgcgc tggcgcatgt acgcgctgct
     5281 cgtcggggct tacgtgctcg tgtatttcca ccgcatggcg cccggcgtcg tcgccggcga
     5341 actgcagcag gccttcgagg ccagcgccgc ggcgctcggc gggctcgccg ccgcgtactt
     5401 ctacatctac gccgcgatgc agctgccggc cggcgtgctc gccgacacct ggggcacgcg
     5461 ccgcaccgtc gtcgccggca acatcgtcgc cgcgctcggg gccgtgctgt tcgcaagcgc
     5521 cggcagcctc gcgcaggcca cgctcgggcg gctggcgatc ggtgccggcg tctcggtcgt
     5581 gttcgtcggc ctgatgaagt tcaacgccgt ctggttcgag ggccgccact acggcaaggt
     5641 cgccggcctg acgctgctga tcggcaacct cggctcgatc gccgccgccg ggccgctggc
     5701 cgggctgctg gcgctggtcg gctggcgcga ggtgttcacg gcgatcggcg gactgtcact
     5761 cgtgctggcg gcgctgacct ggcagttcgt gcgtgatcgc ccggaggagg ccggtctggc
     5821 gccactgcat gtgctgccgc cggccgccgc ccggccgcac tggtcgaagg aactcgccac
     5881 cgtgctgcgc gagcgccggc tgtggccggt ggtcttggtg cagttcggca tgatcg
//
LOCUS       DAOQIE010000253         5915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7422_length_5915_cov_7.994881, whole genome shotgun sequence.
ACCESSION   DAOQIE010000253 DAOQIE010000000
VERSION     DAOQIE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_5915_cov_7.994881"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PK144_14205"
     CDS             <1..1268
                     /locus_tag="PK144_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNF66926.1"
                     /translation="EQRRAAAVAGGAALAPRRAAAEVEAARAAEAAERAVQTATEAAA
                     VLAGLHADRAGLFDGQPTAHVEAALAQAIGQAVAGRDAAQAAVSRAADVRNRAAGQEQ
                     AARSARERADADAAEAARALNDALAEAGLDATEARHRLGFGERWLSDEGAALDALTAA
                     LREAGVRHDERRAQAEAHAAARPPLPAADEGDAPSCPPSSDAPEAQLAGAETALRDAR
                     TALADLAARLRADDQRRSEGAERRTALAARRLQAAPWLALKELIGSADGSKFRGFAQS
                     LTLEALVAHANAHLQELARRYRLRRVPGAGLELMVVDRDMADEVRGVHSLSGGETFLV
                     SLALALGLASLSSNRVQVETLLIDEGFGTLDPDTLDIAVAALDALQGQGRQVGVISHV
                     PALVERIGVQVRVRALGGGRSRIETVTGG"
     gene            1570..2388
                     /gene="pepA"
                     /locus_tag="PK144_14210"
     CDS             1570..2388
                     /gene="pepA"
                     /locus_tag="PK144_14210"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flocculation-associated PEP-CTERM protein PepA"
                     /protein_id="HNF66927.1"
                     /translation="MGTADFGFRTLRAGVAGLLTLMAAAGPAAALTLDETVLSAGATG
                     SVLTDVQQFSIGSSALITPTDPFGGFAELGVFQVTGLFDSAGLPIASTLNTGYGLYLT
                     YVVTGTATPVPGTASMVITYSTANLVLFADRNNDSTFTLIPGGAGPGGLPIDLAIGNM
                     ADDAPIAINNGTNFTGSTTVVPISPTELLGFTDVTLYDWLLQPFGAAALAGDLFPNGL
                     RLVSNTAVPIMPTGVGPILASTQELGTITFIPEPGSLALAAAGFAGLGCRRRRR"
     gene            2403..2876
                     /locus_tag="PK144_14215"
     CDS             2403..2876
                     /locus_tag="PK144_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNF66928.1"
                     /translation="MELPAATRRVRLALEAAGLACRIVRYPAGTRTAADAARAIGCEQ
                     AQIVKSMIFRGEHSGDGLLVLTGGTNRVDPARLEAVLGERPGKADAAFVREWTGFAIG
                     GVPPLGHARPMRTLIDADLLAWPEVWAAAGTPDTVFALPSQALPALTGGTVLRVG"
     gene            complement(2888..3301)
                     /locus_tag="PK144_14220"
     CDS             complement(2888..3301)
                     /locus_tag="PK144_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF66929.1"
                     /translation="MTPFHYAFLVTDLDATRRFYLGMLGCAEGRYSETWVDFDFFGHQ
                     LSAHLGNPLRDPASVGLVDGKEVPMPHFGAILDWEAFHALAARLQTAGVGFVLEPQVR
                     FIGRPGEQATMFLLDPSGNALEFKAFRHPRALFDC"
     gene            complement(3383..4567)
                     /locus_tag="PK144_14225"
     CDS             complement(3383..4567)
                     /locus_tag="PK144_14225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF66930.1"
                     /translation="MKVLMLSDVYFPRVNGVSTSIRTFRRALAAAGHEIDLICPAYGD
                     ADMPEAGVQRIPARRVVFDPEDRMMHARAVLRQADELAGRGYGLIHVQTPFVAHWLGV
                     RLARRLDLPLTETYHTFFEEYLYHYVPLLPRAALRALARTVSRVQCNALDGLVVPSQA
                     MLDKLRGYGIRVPAAVIPTGLDGDSLGSGDGTALRAAHGIAPDRPVLVHVGRLAHEKN
                     VDALLHMLFELRRLVPDVLLLIAGEGPAERHLRALAARLGLDAQVRFLGYLDRAAGLP
                     GCYRAGDAFVFASRTETQGLVLLEAMSLGVPVVSTAVMGTRDIVGPQRGAVAVGEDPV
                     EFAAACARVLGDAALRARLGTEARAFAQGWSATATAGALAGFWQQCIDERLRTTAPCP
                     AR"
     gene            4644..5435
                     /locus_tag="PK144_14230"
     CDS             4644..5435
                     /locus_tag="PK144_14230"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNF66931.1"
                     /translation="MTEETSGRDWLADADTAAGLLRTARRLFVITGAGISADSGLPTY
                     RGIGGLYDDTHTEDAIPIEQALSGSMLARRPAITWKYLAQIERACRGARHNRAHELLA
                     AFDRHFEHVCVLTQNIDGFHRDAGSRHLIEMHGDVHRLLCTACDWRTRIADFDELPPG
                     MPPACPACGEPVRPDVVLFGEMLPEAALSRYYRELDAGFDVVLSIGTTSVFPYIAGPV
                     VAAVQAGIPSIEINPGETSVSRIVDVRIRTGAAAALTALAERIGI"
     gene            5660..>5915
                     /locus_tag="PK144_14235"
     CDS             5660..>5915
                     /locus_tag="PK144_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNF66932.1"
                     /translation="MPTLNWIGKEAVVKHHKEVPFRLLEPVPELSCGDSGSGNLIVQG
                     DNLHALKALLPRYAGQVKCIYIDPPYNTGNEGWVYNDNVNS"
BASE COUNT          910 a         2062 c         2108 g          835 t
ORIGIN      
        1 cagagcagcg ccgggccgcc gccgtcgccg ggggcgcagc gctggccccg cggcgggcgg
       61 cggccgaggt cgaggctgcg cgcgcagccg aagcggcgga gcgggcggtg cagacggcga
      121 cggaggccgc tgcggtcctt gccggactgc acgcggaccg ggccggcctg tttgacggcc
      181 agccgaccgc gcacgtcgag gcggcgctgg cgcaggcgat cgggcaggcc gtggccgggc
      241 gcgatgcagc gcaggcagcc gtgtcccggg ccgcggatgt ccgtaatcgc gcggccggac
      301 aggaacaggc ggcccgcagc gcccgggagc gggccgacgc cgatgcggcc gaagccgccc
      361 gcgcgctgaa cgacgcgctg gccgaagccg ggctcgatgc caccgaggcc cggcaccggc
      421 tgggcttcgg cgagcgctgg ctcagcgacg agggagccgc gctcgatgcg ctgacggctg
      481 cgctgcgcga agccggggtc cggcacgacg aacgacgcgc ccaggccgaa gcccatgccg
      541 cggcacgccc gccgctgccg gccgcggacg agggcgatgc tccgtcgtgc ccgccctcgt
      601 ccgacgcacc cgaagcgcag ctggccggcg ccgagaccgc gctgcgggac gcacgcacgg
      661 ctctcgccga tctcgccgcc cggctgcgtg ccgacgacca gcgccgcagc gaaggcgccg
      721 aacggcgcac ggcgctggcg gcccggcgtc tgcaggccgc gccgtggctg gcgctgaagg
      781 agctgatcgg ttcggcggac ggttcgaagt tccgcggctt cgcgcagagc ctgacgctgg
      841 aagcgctggt cgcgcatgcc aacgcccatc tgcaggaact ggcccgccgc taccgcctgc
      901 gccgcgtgcc gggggccggg ctggagctga tggtcgtcga tcgcgacatg gccgacgagg
      961 tgcgcggcgt gcacagcctt tcgggcggcg agacctttct cgtgtcgctc gcgctcgcgc
     1021 tcggactggc ctcgctgtcc tcgaaccgcg tgcaggtcga gacactgctg atcgacgagg
     1081 gcttcggcac gctcgatccg gacacgctcg acatcgccgt cgccgccctc gacgcgctgc
     1141 aggggcaggg ccggcaggtc ggcgtgatct cgcatgtgcc ggcgctggtc gagcgcatcg
     1201 gcgtgcaggt gcgcgtgcgc gcactcggcg gcgggcgctc gcgcatcgag acggtgacgg
     1261 gcggctgagc cggcgccccc ggagacgagg catatttaat tttgttcatt tgccggcggc
     1321 gggatgcggc cgtgccggtt tatctggttg atgacccatc gtttttgtcg ggattggccg
     1381 tgaacgccat ggcggatggc ctgtttcatg tattttcgtc atggccttac tctcgctgag
     1441 gcatgaggtt gccgattccg gccggcgggc atcggcaccg cagcctcgct tgatcgcaat
     1501 gcagaaccga caaggtgaat caaccagata ttgtccggat tgtccgggaa tgatttgagg
     1561 tgcaaggaaa tgggaacagc tgatttcgga ttcagaaccc tgcgcgcagg ggttgcgggc
     1621 ctgctgacac tgatggcagc ggcagggccg gcagcggcgc tgacgctcga tgaaaccgta
     1681 ctttccgccg gcgccaccgg cagcgtgctg actgacgtac agcagttctc catcggctcc
     1741 agcgcgctga tcacgccgac cgatccgttc ggcggcttcg ccgaactcgg cgtgttccag
     1801 gtcaccggcc tgttcgattc cgccggcctg ccaattgcgt cgacgctgaa caccggctac
     1861 ggcctgtacc tgacctacgt ggtcacgggc accgcgacgc cggtgccggg cactgcgagc
     1921 atggtcatca cttattcgac cgccaacctc gtgctgttcg ccgaccgcaa caacgattcg
     1981 accttcacgc tgatcccggg cggcgccggc cccggcggcc tgccgatcga cctcgccatc
     2041 ggcaacatgg ccgacgatgc gccgatcgcc atcaacaacg gcacgaactt caccggttcg
     2101 acgacggtgg tgccgatttc gccgacggag ctgctgggct tcaccgacgt gacgctctat
     2161 gactggctgc tgcagccctt cggcgccgcg gcactcgccg gtgacctgtt cccgaacggc
     2221 ctgcgcctcg tctccaatac cgccgtgccg atcatgccga ccggcgtcgg tccgatcctg
     2281 gcgtcgacgc aggaactcgg caccatcacc ttcatccccg aaccgggcag tctggcgctg
     2341 gcggctgccg gcttcgcagg actgggctgc cggcgccgtc ggcgctgagc ggaggcgggc
     2401 acatggaact gcctgctgcc acccgacggg tccggctggc tctggaagca gccggactcg
     2461 cgtgtcgcat cgtccgctac ccggccggca cgcgcaccgc agccgatgcg gcgcgggcca
     2521 tcggctgcga acaggcgcag atcgtcaagt cgatgatttt ccgcggtgag cacagcggcg
     2581 atggtctgct ggtgctgacc ggcggcacca accgcgtcga tccggcccgg ctcgaagccg
     2641 tgctcggcga acgccccggc aaggccgatg cggccttcgt gcgcgaatgg accggcttcg
     2701 cgatcggcgg cgtgccgccg ctcggccacg cgcggccgat gcgcacgctg atcgatgccg
     2761 acctgctcgc ctggcccgag gtctgggccg cggccggcac gccggacacg gtgttcgcgc
     2821 tgccctcgca agccctgccg gcattgaccg gcggcacggt gctgcgcgtc ggctgaacgc
     2881 ccgcggttca gcagtcgaac agcgcgcgcg ggtgccggaa agccttgaat tccagcgcgt
     2941 tgccggacgg atcgagcagg aacatcgtcg cctgttcgcc gggccggccg atgaagcgca
     3001 cctgcggctc cagcacgaac ccgaccccgg cggtctgcag ccgggcggcg agcgcgtgga
     3061 aggcttccca gtcgaggatc gcgccgaagt gcggcatcgg cacctccttg ccgtcgacga
     3121 ggccgacgct ggccggatcg cgcagcgggt tgccgaggtg cgcggacagc tggtggccga
     3181 agaaatcgaa atcgacccag gtctcgctgt agcggccctc ggcgcagccg agcatgccga
     3241 gatagaagcg acgcgtggcg tcgagatcgg tgacgaggaa ggcgtaatgg aacggcgtca
     3301 tgccactgcg ctccggccgg cgacgggaaa ccgcagggtc gcccgcccgg gcgctacata
     3361 caagacccct actccatcag ggtcagcgcg cggggcaggg ggcggtggtg cgcaggcgct
     3421 cgtcgatgca ctgctgccag aagccggcga gcgcgccggc ggttgccgtc gccgaccagc
     3481 cctgcgcgaa agcccgggcc tcggtgccga gccgggcacg caacgcggca tccccgagca
     3541 cgcgcgcgca ggcggcggcg aattcgaccg ggtcctcgcc cacggccacg gcgccgcgct
     3601 gcggcccgac gatgtcgcgc gtgcccatga ccgcggtcga caccaccggc acgccgaggc
     3661 tcatcgcctc cagcagcacc aggccctggg tctcggtgcg cgacgcgaac acgaaggcgt
     3721 cgccggcgcg gtaacagccg ggcaggccgg cggcgcggtc gagatagccg aggaagcgca
     3781 cctgcgcatc cagcccgagc cgcgcggcga gcgcacgcag atgacgttcg gccgggcctt
     3841 cgccggcgat cagcagcagc acgtccggca ccagtcggcg cagttcgaac agcatgtgca
     3901 gcagcgcatc gacgttcttc tcgtgcgcga gccggccgac gtgcacgagc accggccgat
     3961 ccggcgcaat gccgtgcgcg gcccgcaacg ccgtaccgtc gccggaaccg aggctgtcgc
     4021 cgtcgagccc ggtcgggatc accgccgccg gcacgcggat gccgtagccg cgcagcttgt
     4081 cgagcatcgc ctgcgagggc acgaccagcc cgtcgagcgc gttgcactgc acgcgcgaca
     4141 ccgtgcgcgc cagcgcccgc agcgccgcgc gcggcagcag cggcacgtag tggtacaggt
     4201 attcctcgaa gaaggtgtgg taggtctcgg tcagcggcag gtcgagccgg cgcgccaggc
     4261 gcacgccgag ccagtgcgcg acgaacggcg tctgcacgtg gatcagcccg tagccgcgcc
     4321 cggccagttc atcggcctgc cgcagcacgg cgcgcgcgtg catcatgcgg tcctcggggt
     4381 cgaacaccac acgccgggcc gggatgcgct gcacgccggc ttcgggcata tcggcgtcgc
     4441 cgtaggccgg gcagatcagg tcgatctcgt ggccggcagc ggccagcgcg cggcggaagg
     4501 tgcggatcga ggtcgacacg ccgttgaccc gcgggaagta cacgtccgac agcatcagga
     4561 ctttcatcga cggcccggtg acagttgcaa acggtcgccg agcctatttc gccgggcttg
     4621 cggtttcgtg acggggagca cgcatgacag aggaaacttc cggccgcgac tggctggccg
     4681 atgccgacac ggccgccggg ctgctgcgca cggcgcggcg gctgttcgtg atcaccggcg
     4741 ccggcatctc ggccgattcg ggcctgccga cctatcgcgg catcggcggc ctgtacgacg
     4801 acacgcacac cgaggacgcg atcccgatcg agcaggcgct gtcgggcagc atgctggcgc
     4861 gccgcccggc gatcacgtgg aagtacctcg cgcagatcga gcgcgcctgc cgcggcgccc
     4921 gccacaaccg cgcgcacgaa ctgctcgcgg cctttgaccg gcacttcgag catgtctgcg
     4981 tgctgacgca gaacatcgac ggcttccacc gcgacgccgg cagccggcac ctgatcgaga
     5041 tgcacggcga cgtgcaccgc ctgctctgca cggcctgcga ctggcgcacg cgcatcgccg
     5101 acttcgacga actgccgccc ggcatgccgc cggcctgccc ggcctgcggc gagccggtgc
     5161 gccccgatgt cgtgctgttc ggcgagatgc tgccggaggc ggcgctgtcg cgttactacc
     5221 gcgaactgga cgccggcttc gacgtggtgc tcagcatcgg cacgaccagc gtgttcccgt
     5281 acatcgccgg cccggtggtc gccgccgtgc aggccggcat cccgagcatc gagatcaacc
     5341 cgggcgagac ttcggtgtcg cggatcgtcg acgtgcgcat ccgcaccggc gccgcggcgg
     5401 cactgacggc gctggccgag cggatcggga tttgatcggc atccggggaa tcccgacgat
     5461 ttcagcccgg tcaggaaacg cagtcgtgat gattttgcaa atggcggatc gcgctgcgtc
     5521 gggccaggcg cagcgttgca tgtaccggtg ccgctgccgg gggcgaacga tcgaaattcc
     5581 gacagattcg gaatcgaatg atgagaagca aatgccgggc ggaattggcg ccctgtgaaa
     5641 ttgaggtaca cgaggcacca tgccaacact gaactggatc ggcaaggaag ccgtcgtcaa
     5701 gcatcacaag gaagtgccat tccgactgct ggagccggtg ccggagcttt cctgtggcga
     5761 cagcgggagt ggcaacctca ttgtgcaggg cgacaacctg cacgcgctca aggcgctgct
     5821 gccccgatac gccggtcagg tgaagtgcat ctatatcgat ccgccctaca acaccggcaa
     5881 cgagggctgg gtttacaacg acaacgtcaa cagcc
//
LOCUS       DAOQIE010000254         5885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7475_length_5885_cov_7.629503, whole genome shotgun sequence.
ACCESSION   DAOQIE010000254 DAOQIE010000000
VERSION     DAOQIE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7475_length_5885_cov_7.629503"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PK144_14240"
     CDS             <1..1484
                     /locus_tag="PK144_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNF66933.1"
                     /translation="WLAPVRAGALAALLSALGYALIAGLGLPVRRALIMVAVGSLALL
                     AGRELLSTRTLALALIAVLLGDPLAPLSYGFWLSFGAVALILYLHAGRLDGRGPTALA
                     LRLQWAISLGMLPATIVLFGQLPLVSPLANLLAIPWADLLIVPFALAGLLASLASDTL
                     AGPLLDLAGLTTRLLLGALDTLARPDWAARVVPTPPLWTLALALPGVAWWLAPPGFPS
                     RRLGLLLCLPLLAWDWPHPPAGGFRLASLDAGSGLAVVVETAGHTLLFDAGPRLGSTD
                     AGRLVVLPYLQSRGLRRVDALVLTHLDASHIGGARSVRAALPVGAIFVPDPLAMPAEG
                     AASCVAGGFWIWDDVRFEWLHPPPGHGAGEARSCVLKVSGAGGSALLIADLDAGGSRR
                     LLAGGARLAADTLLAPYQGTKPPPAGLLAAVGPGRIWLSTGRGNRWGHPKPEVLAAWA
                     AAGAQLDDTAAAGALLLDVPANATEPPLLTRWREADRRYWRVH"
     gene            1643..2356
                     /locus_tag="PK144_14245"
     CDS             1643..2356
                     /locus_tag="PK144_14245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF66934.1"
                     /translation="MQLSVVVPVHNEGENIAPLIDEIVAALAGREDYEIIYVNDGSRD
                     DTAARLAEAATRLPQLRVITHARACGQSTAVATGIRHARGRWIATLDGDGQNDPADIP
                     KLLAVLTDPTRPLKRVMAAGYRRKRQDTWIKRISSKIANGVRSRLLGDATPDTGCGLK
                     VFERAVFLELPYFDHMHRFLPALVRRAGYELVSVEVNHRHRTRGVSKYGTLDRLAVGI
                     VDLFGVMWLQRRARVPEIE"
     gene            2356..3042
                     /locus_tag="PK144_14250"
     CDS             2356..3042
                     /locus_tag="PK144_14250"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNF66935.1"
                     /translation="MFKLDPIWFVVGLVAQAAFSARFLVQWILSERARRSLMPIHFWY
                     FSIAGSTLLLAYATHQRDAVIVLGQAAGLAIYLRNLEIIHRHERGHGTFFLWPWLTLA
                     SLALATGWASTPLPVTHALTNAHPLWSTVGMIGQCLFTGRFVVQLYFSERAGRPVNPI
                     HFWYLSMSGSLLLLAYALFQRDPVIILGQSFGVIVYLRNLALIRQHRQADRHGEDDTG
                     TTTVTGSSTA"
     gene            3128..4801
                     /locus_tag="PK144_14255"
     CDS             3128..4801
                     /locus_tag="PK144_14255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF66936.1"
                     /translation="MSTAPTAGAPLRTLLHLAGWLAVWGLLTAVALSTRSLVPIDETR
                     YAAVAWEMWARGDGLVLHLNGEPYSHKPPLLFWLIELSWAVFGIGDAQARLVTALAAF
                     ANLPLLAVLAARLWPQDPGPRRCAPWLLYGGLYYALFGTMLMFDLLMSVCVLLGLTGL
                     AGAWRSGGRRRDWLLYALGLGLGGLTKGPAVLVYLLPPALLAPLWIVEARPDWRRWYR
                     ALGLSLLGGFALALAWAVPAAIAGGAEYRGAILWGQTAGRMVQAFAHQRPFWWYLPVL
                     PLILLPWTLWLPLWRALPALRTEAGGSALRFCLACFVPGFLIFSAVSGKQVHYLLPLL
                     PALLLPAARALTLGACDRQPGRLAVALVIAPVVGLGVLLALAPSFVDHYRWPDWVGTV
                     SPLWGLAVAGLGLAWAPLLRTRRAGLWLAVLPALLGTLLYAGIARPATAALDGSPVAQ
                     AIGRLQAEGVTVAHPGKYHGEFQFPGRLRQPLEVITRGSELEWARAHPQGALIVRHDE
                     LDPEQAALAVIVQPYRQRYAALWRAQTLIEHPGLLAKESGESRGSEGEE"
     gene            4876..>5885
                     /gene="dusA"
                     /locus_tag="PK144_14260"
     CDS             4876..>5885
                     /gene="dusA"
                     /locus_tag="PK144_14260"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNF66937.1"
                     /translation="MPPAAGAPHDPLAPARRLSVAPMLDWTTRDFRYFARLLSQRVLL
                     YTEMVTTGAILHGDPARHLGFDAAEHPLALQLGGSDPQALAACARLAVDDFGYDEINL
                     NCGCPSDRVQEGRFGACLMAEPPLVADCVAAMRAAVPAHVPVTVKTRIGIDELDRYEQ
                     LLHFVETVAAGGCTVFVIHARKAWLSGLSPKENREIPPLRYEVAAQLKRDCPQLSISL
                     NGGVQTLAQARQHLEWADGVMIGRAAYENPWLLAGADAALWGDAPMPRSRHDVLAAFE
                     PYVERHLARGGRLAHLTRHLLGLFQGVPGARAWRQYLSTHAHRHGAGLDVLRAAAAKV
                     SR"
BASE COUNT          686 a         2222 c         2061 g          916 t
ORIGIN      
        1 cgtggctggc gccggtgcgc gccggcgcgc tggcggcgct gctgagcgcg ctcggctacg
       61 cgctgatcgc cggcctcggc ctgccggtgc gccgggcact gatcatggtc gcggtcggca
      121 gcctcgcgct gctcgccggg cgcgagctgc tgtcgacgcg cacgctggcg ctggcgctca
      181 tcgccgtgct gctcggcgat ccgctggcgc cgctgtccta cggcttctgg ctgtccttcg
      241 gcgcggtggc actgatcctg tacctgcacg ccggccgtct ggacgggcgc gggccgacgg
      301 cactggcgct gcgcctgcag tgggcgatca gtctcggcat gctgccggcg accatcgtgc
      361 tgttcggaca gctgccgctg gtgtcgccgc tcgcgaacct gctggcgatc ccgtgggcgg
      421 atctgctgat cgtgccgttc gcgctggccg ggctgctcgc gagtctggcg tccgacacgc
      481 tggccggacc gctgctcgat cttgccggac tcacgacccg gctgctgctc ggcgcgctcg
      541 acaccctcgc ccgccccgac tgggcggcgc gcgtcgtgcc gaccccgccg ctgtggacac
      601 ttgcgctggc gctgcccggc gtcgcgtggt ggctggcgcc gccgggcttt ccgtcgcgcc
      661 ggctcgggct gctgctctgc ctgccgctgc tcgcctggga ctggccgcat ccgccggccg
      721 gcggcttccg gctcgcctcg ctcgatgccg gcagcggcct cgccgtcgtc gtcgagaccg
      781 ccggccacac gctgctgttt gacgccggcc cgcggctcgg ctcgaccgat gccggccggc
      841 tcgtcgtgct gccctacctg cagtcccgcg gcctccgccg ggtcgatgcg ctggtgctga
      901 cacacctcga cgccagccac atcggcggtg cgcgcagcgt gcgcgccgcg ctgccggtcg
      961 gcgcgatctt cgtgcccgac ccgctggcga tgccggccga gggcgccgca agctgcgtgg
     1021 ccggcggctt ctggatctgg gatgacgtgc gcttcgaatg gctgcatccg ccgcccggtc
     1081 acggtgccgg cgaggcccgc agctgcgtgc tgaaggtcag cggcgccggc ggttcggcgc
     1141 tgctgatcgc cgatctcgat gccggcggca gccgccggct gctggccggc ggcgcccggc
     1201 ttgcggccga tacgctgctc gccccgtatc agggcacgaa gccacccccg gccggcctgc
     1261 tggccgcggt cgggccggga cgcatctggc tgtccacggg ccgcggcaac cgctggggac
     1321 atccgaaacc cgaagtgctc gcagcctggg cagcagccgg cgcgcagctc gacgacaccg
     1381 ccgcggccgg agcgctgctg ctcgacgtgc cggcgaacgc cacggagccg ccgctcctca
     1441 cgcgctggcg cgaagccgat cgccgctact ggcgggtgca ctgaagcctg ccgtctgcgc
     1501 ccgcgcggtg cagaccgtcg cccccatggg cccgaagcgg gccagaagcg ggcaaagggg
     1561 gtgtggtgcc attgatattg cactgcaaca tcgcggtgca tcgcgtagaa tccgcgcact
     1621 tttttccgcc gcggtcaccc cgatgcagct gtcagtcgtc gtccccgtcc acaacgaagg
     1681 cgaaaacatc gcgccgctga tcgacgagat cgtcgccgcc ctggccgggc gcgaggacta
     1741 cgagatcatt tacgtcaacg atggcagccg cgacgacacg gcggcgcgcc tggccgaagc
     1801 cgccacgcgc ctgccgcagc tgcgcgtgat cacgcacgcc cgcgcctgcg gccagagcac
     1861 ggcggtcgcc accggcatcc gccacgcgcg cggacgctgg atcgcgacgc tcgatggcga
     1921 cggccagaac gatccggccg acatcccgaa gctgctcgcg gtgctgaccg atccgacgcg
     1981 cccgctgaaa cgcgtcatgg ccgccggtta ccgcaggaag cgtcaggaca cctggatcaa
     2041 gcgcatctcc tcgaagatcg ccaacggcgt gcgctcgcgc ctgctcggcg atgccacgcc
     2101 ggacaccggc tgcggcctga aggtcttcga acgcgcggta ttcctcgaac tgccctactt
     2161 cgaccacatg caccgcttcc tgccggcact ggtgcgtcgc gccggctacg agctggtgtc
     2221 ggtcgaggtc aaccaccgtc accgcacgcg cggcgtgtcc aagtacggca cgctcgaccg
     2281 gctcgccgtc ggcatcgtcg acctgttcgg cgtgatgtgg ctgcagcggc gcgcgcgcgt
     2341 gccggagatc gaatgatgtt caagctggac ccgatctggt tcgtcgtcgg actcgtcgcg
     2401 caggcggcgt tctcggcacg cttcctcgtg cagtggatcc tgtccgagcg cgcccggcgc
     2461 agcctgatgc cgatccactt ctggtacttc agcatcgccg gctcgacgct gctgctcgcg
     2521 tatgcgaccc accagcgcga tgccgtcatc gtgctcggac aggcggccgg cctggcgatc
     2581 tacctgcgca acctcgaaat catccaccgc cacgagcgcg gacacggcac gttcttcctg
     2641 tggccgtggc tgacgctcgc gagcctggcg ctcgccaccg gctgggcctc gacgccgctg
     2701 ccggtcacgc atgcgctgac caatgcgcat ccgctgtggt cgacggtcgg catgatcggc
     2761 cagtgcctgt tcaccggccg cttcgtcgtg cagctgtact tctcggagcg cgccgggcgg
     2821 ccggtcaatc cgatccattt ctggtatctg tcgatgagcg gcagcctgct gctgctcgcc
     2881 tatgcgctgt tccagcgcga cccggtcatc atcctcggcc agagcttcgg cgtgatcgtc
     2941 tacctgcgca atctggcgct gatccgccag caccggcagg ccgaccgtca cggcgaggac
     3001 gacaccggga cgactacggt gaccggcagc tcgaccgcct gagcccgcgg cggcgtttcg
     3061 ccgacgcctg attccgcccc cgcgcccggc ctgcaccggg cgtcgtcatt tccacccgga
     3121 gcccccgatg tccaccgccc cgaccgccgg cgccccgctg cgcacgctgc tgcacctcgc
     3181 cggctggctc gccgtctggg gcctgctgac ggcggtcgcg ctgtcgacgc gctcgctggt
     3241 gccgatcgac gagacgcgct atgcggccgt cgcctgggag atgtgggccc gtggcgacgg
     3301 gctggtgctg cacctcaacg gcgaaccgta cagccacaag ccgccgctgc tgttctggct
     3361 gatcgagctg tcgtgggcgg tgttcggcat cggcgatgcg caggcgcggc tggtcacggc
     3421 gcttgcggcc ttcgcgaacc tgccgctgct cgccgtgctg gccgcccggc tgtggccgca
     3481 ggatcccggc ccgcgccgct gcgcaccgtg gctgctgtac ggcggcctgt actacgcgct
     3541 gttcggcacg atgctgatgt tcgacctgct gatgagcgtc tgcgtgctgc tcggcctgac
     3601 cgggctcgcc ggtgcctggc gctcgggcgg gcggcggcgc gactggctgc tgtacgcgct
     3661 cggcctcggg ctcggcgggc tgaccaaggg accggccgtg ctcgtctacc tgctgccgcc
     3721 ggcgctgctc gccccgctgt ggatcgtcga ggcccgcccc gactggcggc gctggtatcg
     3781 cgcactcggc ctgtcgctgc tcggcggctt cgcgctcgcg ctcgcctggg cggtgccggc
     3841 ggcgatcgcc ggcggtgccg agtaccgcgg cgcgatcctg tgggggcaga cggccggacg
     3901 catggtgcag gctttcgcgc atcagcggcc gttctggtgg tatctgccgg tgctgccgct
     3961 gatcctgctg ccgtggacac tatggctgcc gctgtggcgc gcgctgccgg cgctgaggac
     4021 cgaagccggc ggcagcgccc tgcgcttctg cctcgcctgc ttcgtgcccg gcttcctgat
     4081 cttctcggcg gtcagcggca agcaggtgca ttacctgctg ccgctgctgc cggcgctgct
     4141 gctgccggca gcgcgcgcgc tgacactcgg cgcctgcgat cgccagcccg gacgcctcgc
     4201 ggtcgcgctc gtcatcgcgc cggtcgtcgg actcggcgtg ctgctcgcgc tggcgccgtc
     4261 cttcgtcgat cactaccgct ggccggactg ggtcggcacg gtgtcgccgc tctggggcct
     4321 tgcggtggca gggctcggtc tggcctgggc accgctgctg cgcacgcgcc gggccgggct
     4381 gtggctcgcg gtcctgccgg ccctgctcgg cacgctgctg tatgccggca tcgcccggcc
     4441 ggcgaccgcc gcactcgacg gcagcccggt ggcgcaggcg atcggccggc tgcaggccga
     4501 aggcgtcacg gtcgcgcatc ccggcaagta ccacggcgaa ttccagttcc cgggccggct
     4561 gaggcagcca ctcgaagtca tcacgcgcgg cagcgagctc gaatgggcgc gcgcgcaccc
     4621 gcagggcgcg ctgatcgtgc gccacgacga gctcgatccg gagcaggccg cgctcgcggt
     4681 catcgtccag ccctaccgcc agcgctacgc ggccctctgg cgcgcgcaga cgctgatcga
     4741 gcaccccgga ctgctggcga aggaaagcgg cgagagccgt ggcagcgagg gcgaggaatg
     4801 atgaccggcc cgctcgcagc cgccccggat gccgcagcac cgcagcagga tcggagcccg
     4861 cgcaacggcg ccggcatgcc gcccgctgcc ggcgcgccgc atgacccgct cgcccctgcc
     4921 cgccgcctgt cggtcgcgcc gatgctcgac tggacgacgc gcgacttccg ttacttcgcg
     4981 cgcctgctgt cgcagcgcgt gctgctgtac accgagatgg tcacgaccgg cgccatcctg
     5041 cacggcgacc cggcccggca cctcggcttc gacgccgccg aacacccgct ggcgctgcag
     5101 ctcggcggct ccgacccgca ggccctcgcc gcctgcgcgc gcctcgcggt cgatgacttc
     5161 ggctacgacg agatcaacct gaactgcggc tgcccgtcgg accgcgtgca ggaagggcgc
     5221 ttcggcgcct gcctgatggc cgagccgcca ctcgtcgccg actgcgtggc cgcgatgcgc
     5281 gccgcggtgc cggcacacgt gccggtgacc gtgaagacgc gcatcggcat cgacgagctc
     5341 gaccgctacg agcagctgct gcacttcgtc gaaaccgtcg ccgccggcgg ctgcacggtg
     5401 ttcgtgatcc acgcgcgcaa ggcctggctc tcgggcctgt cgccgaagga aaaccgcgag
     5461 atcccgccgc tgcgctacga ggtcgccgcg cagctcaagc gcgactgccc gcagctttcg
     5521 atcagcctca acggcggcgt gcagacgctg gcgcaggccc gccagcacct cgaatgggcc
     5581 gacggcgtca tgatcggccg cgccgcctac gagaacccct ggctgctcgc cggagccgat
     5641 gcggccctct ggggcgatgc cccgatgccg cgcagccgcc acgacgtgct cgccgccttc
     5701 gaaccctacg tcgaacggca tctggcccgc ggcgggcgac tcgcacacct cacgcgccac
     5761 ctgctcggtc tgttccaggg cgtgcccggt gcgcgcgcgt ggcggcaata tctgtcgacg
     5821 cacgcgcacc ggcacggcgc cggcctcgac gtgctgcgcg cggcagcggc gaaggtgtcg
     5881 cgcga
//
LOCUS       DAOQIE010000255         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7794_length_5714_cov_7.446192, whole genome shotgun sequence.
ACCESSION   DAOQIE010000255 DAOQIE010000000
VERSION     DAOQIE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_5714_cov_7.446192"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="prpE"
                     /locus_tag="PK144_14265"
     CDS             complement(<1..1549)
                     /gene="prpE"
                     /locus_tag="PK144_14265"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HNF66938.1"
                     /translation="MTYETFHKRSIDDMEGFWGEQAGLIHWNKPPQQVLDYSRPPFRK
                     WFVGGETNLCYNAVDRHLAARADQPALVYISTETEETKSYSFAELHREVNRTAAILVA
                     LGVGKGDRVVIYMPMIAEALFAMLACARLGAVHSVVFGGFASHNLAVRIDDAQPKLLV
                     TADAGMRNGKPVPYKHLVDEACALAKYPPQHVLIVNRGLDPQMPITEGRDADYATLRE
                     QHLDAEVPVTWVESNEPSYILYTSGTTGTPKGVQRDTGGYCVALAASMKHIYNANPGE
                     TMFTTSDIGWVVGHSYIVYGPLIHGMTTIVYEGLPTRPDAGIWWKIVEDYKVTTMFSS
                     PTAVRVLKKQDPEYLRKYDLSTLRYLFLAGEPLDEPTSRWISDALGVPVIDHYWQTET
                     GWAMLTYLPGVDLKPNRFGSPGFPNYGFNIRVINEATGADAEANEKGVLVVVPPLPPG
                     CLTTVYGNDERFVKSYFSQFKELLYTSSDWAVRDDDGYYFILGRTDDVINVAGHRLGT
                     REIEEAVQ"
     assembly_gap    1696..1795
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1881..2864)
                     /locus_tag="PK144_14270"
     CDS             complement(1881..2864)
                     /locus_tag="PK144_14270"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNF66939.1"
                     /translation="MKNPVRVAITGAAGNIGYAMAFRIAAGDMLGPDQPVILHLLEIT
                     PALPALQGVVMELNDCAFPLLAGVVATDKLEEAFKDVNYALLVGARPRGPGMERKDLL
                     TANGAIFGPQGKALNDVASRDVKVLVVGNPANTNSLIAQCNAPDLDPKQFTAMTRLDH
                     NRALSQLAEKTGVHFNFIKKMTIWGNHSSTQYPDISQCTVDGKAAAELVDSDWYKSTF
                     IPDVQQRGAAIIKARGASSAASAASAAIDHMRSWALGTAEGDWVSMGIPSDGSYGIAP
                     GVIYSYPCVCKDGKYEIVQGLPVDEFSRAKMDATEAELREERAAIEHLLKP"
     gene            complement(3137..3991)
                     /locus_tag="PK144_14275"
     CDS             complement(3137..3991)
                     /locus_tag="PK144_14275"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNF66940.1"
                     /translation="MSLPKRMAFFVSDRTGITAETLGHSLLTQFESLNIEKQAVRFVD
                     TPERAKALRDRIDRHFREDGLKPLVFSTLIAEETRAIVKQANGHFIDFFEVFIGPLEQ
                     ELGVKSSLTAGRSHGLTDMVEYSRRIDAVNFTLNHDDGSTTAHLAEADIILVGISRTG
                     KTPTCLYLSLHYGIRAANFPLTEDDLEDMKLPNSLRPYQDKLFGLTTDPMRLAQIRHE
                     RMPNSKYSSLEQCDYETRQAEALFRKLGVPYLRTTMKSIEEIATTIIDMAGLEKRKVR
                     PHRPQAHV"
     assembly_gap    4016..4115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4116..4408)
                     /locus_tag="PK144_14280"
     CDS             complement(<4116..4408)
                     /locus_tag="PK144_14280"
                     /inference="COORDINATES: protein motif:HMM:NF013853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNF66941.1"
                     /translation="MRYESDFVDWTFEQARLLRAGDWSALDVERIAEELEAMGRSERR
                     ALVHRLEVLLMHLLKWQFQPGRRSPSWRSTIRIQRIEVAELLADNPSLKPQLDA"
     gene            complement(4479..>5714)
                     /locus_tag="PK144_14285"
     CDS             complement(4479..>5714)
                     /locus_tag="PK144_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020751064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNF66942.1"
                     /translation="VNEMNNNWWCENIRATNPCGEQPLPPYGSCLLGSVDLTRFVLEP
                     FTERARFDWDTYREVVSVFTRMLDNVVEINGLPLEPQRAEITGKRRHGMGYLGLGSTM
                     TLLRMRYGSAESLAFTEQVTREMAVAGWRTALELAQEKGPAPVLAAEYVVDGEMLRKR
                     PEMARDGFQAGDRVPGRVLHAKYSRYMQRLAGVEPALVEALANVGARFTHHTSIAPTG
                     TISLSLANNASNGIEPSFAHHYARNVIVPGKKSKEKVDVWSFELLAYRELVNPQAMPY
                     SGDAAAQLPEYFIAADDVTPKQHVDVQAAAQKWVDSSISKTANVPTDFRYEDFKDIYL
                     YAYEQGLKGCTTFRFNPEAFQGVLVKEKDLENTLYRFTLADGQVVEVRGNEQIEYDGE
                     LHTAANLFDALKEGYYGKF"
BASE COUNT          860 a         1772 c         1876 g         1006 t
ORIGIN      
        1 cctgcacggc ttcctcgatc tcgcgggtgc cgaggcggtg gccggcgacg ttgatcacgt
       61 cgtcggtgcg cccgaggatg aagtagtagc cgtcgtcatc gcgcacggcc cagtccgagg
      121 aggtgtacag cagctccttg aactggctga agtagctctt cacgaagcgc tcgtcgttgc
      181 cgtagaccgt cgtgaggcag cccggcggca gcggcggcac gacgaccagc acgcccttct
      241 cgttcgcctc ggcgtcggca ccggtggctt cgttgatgac gcggatgttg aagccgtagt
      301 tcgggaagcc cggcgagccg aagcggttcg gcttcaggtc cacgcccggc aggtaggtca
      361 acatcgccca gccggtctcg gtctgccagt agtggtcgat gaccggcacg ccgagcgcat
      421 cgctgatcca gcgcgaggtc ggctcatcga gcggctcgcc ggccaggaac aggtagcgca
      481 gcgtcgagag gtcgtacttg cgcaggtact ccgggtcctg cttcttcagc acgcgcaccg
      541 cggtcggcga gctgaacatc gtcgtgacct tgtagtcctc gacgatcttc caccagatgc
      601 cggcatcggg ccgcgtcggc agaccctcgt agacgatggt cgtcatgccg tggatcagcg
      661 ggccgtagac gatgtagctg tggccgacga cccagccgat gtccgaggtc gtgaacatcg
      721 tctcgccggg attggcgttg tagatgtgct tcatcgacgc ggcgagcgcg acgcagtagc
      781 cgccggtgtc gcgctgcacg cccttcggcg tgccggtggt gccggaggtg tagaggatgt
      841 agctcggctc gttcgactcg acccaggtca ccggcacttc ggcgtcgagg tgctgctcgc
      901 gcagcgtcgc gtagtcggca tcccggcctt cggtgatggg catctgcggg tcgaggccgc
      961 ggttgacgat cagcacgtgc tgcggcgggt acttggcgag cgcgcaggcc tcgtcgacca
     1021 ggtgcttgta cggcaccggc ttgccgttgc gcatgccggc atcggcggtg acgagcagct
     1081 tcggctgcgc atcgtcgatg cgcacggcga ggttgtgcga cgcgaagccg ccgaacacca
     1141 ccgagtgcac ggccccgagg cgcgcgcagg ccagcatcgc gaacagcgcc tcggcgatca
     1201 tcggcatgta gatgacgaca cggtcgccct tgccgacgcc gagcgcgacg aggatcgccg
     1261 cggtgcggtt gacctcgcgg tgcagctcgg cgaacgagta cgacttcgtc tcttcggttt
     1321 cggtcgagat gtagaccagc gccggctgat cggcgcgcgc agccagatga cggtcgaccg
     1381 cgttgtagca gaggttggtc tcaccgccga cgaaccactt gcgaaacggc ggtctggaat
     1441 agtcgagcac ctgctgcggc ggcttgttcc agtggatcag gccggcctgc tcgccccaga
     1501 agccttccat gtcgtcgatg gatcgcttgt ggaaagtttc gtacgtcatc ccgtttcccc
     1561 tccaaatctc tggtacggca tcggtcgtgg gttgccgcct tgcggcggca ggggcggcag
     1621 gtgcgcccgc ggtttccggc ctgcgcgctt ccggatcagc ggagggcggc agcggaaacc
     1681 gtttgttgtt gtgttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggaa
     1801 gacggccaaa acaaaaggcg ccccgcaggg cgccttgtcg gaggttgacg cttacgtcaa
     1861 cgtcaatgct gcaggccgga tcacggcttc agcagatgct cgatcgccgc gcgctcctcg
     1921 cgcagttcgg cttcggtggc gtccatcttg gcgcgcgaga actcatcgac cggcagaccc
     1981 tggacgatct cgtacttgcc gtccttgcag acgcagggat acgagtagat gacgcccggc
     2041 gcgatgccgt aggagccgtc ggacgggatg cccatcgaca cccagtcgcc ctcggcggtg
     2101 ccgagcgccc acgaacgcat gtggtcgatc gcggccgagg cggccgaggc ggccgacgag
     2161 gcgccgcggg ccttgatgat cgccgcgccg cgctgctgca cgtcggggat gaaggtgctc
     2221 ttgtaccagt cgctgtcgac cagctcggcg gcagccttgc cgtcgacggt gcactgcgag
     2281 atgtccggat actgggtgct ggagtggttg ccccagatcg tcatcttctt gatgaagttg
     2341 aagtgcacgc cggtcttctc ggcgagctgc gacagcgcac ggttgtggtc gaggcgggtc
     2401 atcgccgtga actgcttcgg atcgaggtcc ggggcgttgc actgggcgat cagcgagttg
     2461 gtgttggccg ggttgccgac cacgagcacc ttcacgtcac gcgaggcgac atcgttcagc
     2521 gccttgccct ggggcccgaa gatcgcgcca ttggcggtca gcaggtcctt gcgctccatg
     2581 cccgggccgc gcggacgggc gccgaccagc agcgcatagt tcacatcctt gaaggcttct
     2641 tcgagcttgt cggtggcaac gacaccggcc agcagcggga aggcgcagtc gttgagctcc
     2701 atcacgacgc cctgcagcgc cggcagcgcc ggggtgattt cgagcaggtg caggatgacc
     2761 ggctggtccg ggcccagcat gtcgccggcg gcgatgcgga aagccatggc ataaccgatg
     2821 ttgccggcgg caccggtgat ggcgacgcga accggattct tcatgtcatt gcgctcctaa
     2881 gtcgttctgc gtttgtggcc ccgcatcggg cgggacccgc actgaaaagt ctgctgcttg
     2941 cgagcaaacc ctcggcgccg gcgagagcat gatgctcacc ggcgccagat tcagatgccg
     3001 agcgatttcg gcacccccct tgcctggacg cgcgccccgt tgtaccccgg ggctccggac
     3061 cgcggcatat cctgcatgat gcgccgtcaa atgcaaccgc gccggtcagg gaggcgcgct
     3121 gcaagcccgc ggaaaatcag acgtgcgcct gcggccggtg cggccgcacc ttgcgctttt
     3181 cgagcccggc catgtcgatg atggtcgtcg cgatttcctc gatggatttc atcgtcgtgc
     3241 gcaggtacgg cacgccgagc ttgcgaaaca gcgcctcggc ctggcgggtc tcgtagtcgc
     3301 actgttcgag cgacgagtac ttgctgttcg gcatgcgctc gtggcggatc tgcgcgagcc
     3361 gcatcgggtc ggtggtcagc ccgaacagct tgtcctggta ggggcgcagg ctgttcggca
     3421 gcttcatgtc ctccaggtcg tcctcggtca gcgggaagtt ggcggcgcgg atgccgtagt
     3481 gcagcgacag gtacaggcag gtcggcgtct tgccggtgcg cgagatgccg accaggatga
     3541 tgtcggcctc ggccagatgc gcggtggtcg aaccatcgtc gtggttcagc gtgaagttca
     3601 cggcgtcgat gcgccgcgag tactcgacca tgtcggtcag gccgtgcgag cgtccggcgg
     3661 tcagcgagga cttgacgccg agttcctgct ccagcggccc gatgaacacc tcgaagaagt
     3721 cgatgaagtg gccgttcgcc tgcttgacga tcgcgcgcgt ctcctcggcg atcagcgtcg
     3781 agaacaccag cggcttgagc ccgtcctcgc gaaagtgccg gtcgatgcga tcgcgcagcg
     3841 ccttcgcccg ctcgggcgta tccacgaagc gcaccgcctg cttctcgatg ttcagcgact
     3901 cgaactgcgt cagcaggctg tgcccgagcg tctcggcggt gatgccggtg cgatcggaga
     3961 cgaagaaggc catgcgcttg ggcagggaca tgggcgggct ccagcggcga ggacgnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcatc gagctgcggc ttcaggctcg
     4141 gattgtcggc cagcagctcg gccacctcga tgcgctggat gcggatcgtg ctgcgccagc
     4201 tcgggctgcg tctgccgggc tggaactgcc acttcagcag atgcatcagc agcacttcga
     4261 gacgatgcac cagcgcccga cgctcgctgc gccccatggc ctccagctcc tctgcaatgc
     4321 gttcgacgtc gagagccgac cagtcgcccg cccgcagcag ccgcgcctgc tcgaaggtcc
     4381 agtccacgaa atccgactcg tatcgcacgc tgcccactcc tgccccccgc ccgtcacgca
     4441 cccggcagtc cggccgccgg atgcgcagga ctcaccgttc agaacttgcc gtaataccct
     4501 tccttcagcg catcgaacag attggccgcg gtgtggagtt caccgtcgta ttcgatctgc
     4561 tcgttgccgc ggacctcgac gacctgaccg tcggcgagcg tgaagcggta cagcgtgttt
     4621 tcgagatcct tctccttgac cagcacgccc tggaaagcct cggggttgaa gcggaaggtc
     4681 gtgcagccct tcaggccctg ttcgtatgcg tacaggtaga tgtccttgaa atcctcgtag
     4741 cggaaatcgg tcggcacgtt cgcagtcttc gagatgctcg aatcgaccca cttctgcgcg
     4801 gcggcctgca cgtcgacgtg ctgcttcggc gtcacgtcat cggcggcgat gaagtactcc
     4861 ggcagttgcg cggcggcgtc cccggaatac ggcatcgcct gcggattgac cagttcgcgg
     4921 taggccagca gttcgaagga ccagacatcg accttctcct tgctcttctt gcccggcacg
     4981 atgacgttgc gtgcgtaatg gtgcgcgaag ctcggttcga tgccgttcga ggcgttgttg
     5041 gcaaggctca gcgagatcgt gccggtcggt gcgatcgagg tgtggtgcgt gaagcgtgca
     5101 ccgacgttcg cgagcgcctc gaccagcgcc ggctcgaccc cggcgagacg ctgcatgtag
     5161 cggctgtact ttgcatgcag cacacggccc ggcacgcggt cgccggcctg gaaaccgtcg
     5221 cgcgccattt ccgggcgctt gcgcagcatc tcgccatcga cgacgtattc ggccgcgagc
     5281 accggggccg gacctttctc ctgcgccagt tccagcgccg tgcgccagcc ggcgacggcc
     5341 atctcgcgcg tgacctgctc ggtgaacgcc agcgattccg ccgagccgta gcgcatgcgc
     5401 agcagcgtca tcgtcgagcc gaggccgaga tagcccatgc cgtgacggcg cttgccggtg
     5461 atctcggcgc gctgcggctc cagcggcagg ccgttgatct cgacgacgtt gtcgagcatg
     5521 cgcgtgaaca ccgacaccac ctcgcggtag gtgtcccagt cgaagcgcgc acgctcggtg
     5581 aacggttcga gcacgaagcg cgtcaggtcg accgagccga gcaggcagga accgtacggc
     5641 ggcagcggct gctcgccgca gggattggtg gcgcggatgt tctcgcacca ccagttgttg
     5701 ttcatctcgt tgac
//
LOCUS       DAOQIE010000256         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7928_length_5660_cov_7.586619, whole genome shotgun sequence.
ACCESSION   DAOQIE010000256 DAOQIE010000000
VERSION     DAOQIE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7928_length_5660_cov_7.586619"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..2191
                     /locus_tag="PK144_14290"
     CDS             182..2191
                     /locus_tag="PK144_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF66943.1"
                     /translation="MPQSPRQSATAGIVAAGVLLVAATAAGLWAIQKKHAIDLEATRA
                     AYIQESHQRLLARREQVEIELGALARGLRAIARLPAVQAQGPAGAAEPETAAAIGEIF
                     LGQQRLRPQRLLVVGTGSDAPMPYRLDAVTVLPWTAPRKAASADCTARSDALLDERLR
                     GQLAYLQQQHPRANAVPWSAYPAIGARAVPDCLDPDGAGHFLWTVPVYATDGTLSGAV
                     AVVLPSRALAAAIGAGAEDGNFALYNADHDYLVAGGPGGEAGRAAGWIRQQRAAPDTI
                     YSETMPLGLAGAARGWLVWYGRPDSDWATRPDVLGADRFHRLAQAVVIGLAVVAALAL
                     ALGARRRRVLERLVAERTRELHESQAALVQAGRLEGLGTLAAGIAHEIKNPLAWTRSN
                     LDVCVDHARTLASLSKDWREAQQPGADTDARALKLGRTLIAAETFVREETATELMQLL
                     EDCADGTTRIQAIVEAVGSFSRLDQRQVASTEIPKLVASVLSIAGSEIRKCGARIDTR
                     YAEDLPAIICAPGQLAQVLLNLVVNACHAMQGAAEHRLTISAELHARGRRVRLVVSDT
                     GCGIPAEIRDHIFDPFFTTKDVGKGTGLGLHLAHQIVRAHGGRILVDSTPGHGASFTL
                     DLPVDASAELAAAAPVDAAATTAAAPRPAGNPDPSEPLRPLGAAA"
     gene            2202..3224
                     /locus_tag="PK144_14295"
     CDS             2202..3224
                     /locus_tag="PK144_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF66944.1"
                     /translation="MSTTPHLVPVPVSGTRPQPAGSKLPTVLFVDDEPPITRSLSALF
                     RRTGQYAVLTANSARDALELLGTHDIDVIVSDQRMPGMTGVELLREFATRSPRTMRIL
                     LTAWSDLSAITASINEGEVFRFVQKPWGNDLLATVAVAASASLAPANEPVHGAPDLVP
                     AAAQGTGVGILVVDDGRELLDLVRADFGSTHPVHVARHADQAIALLEQHEGIGIVVSD
                     VRVADEDMTDVIKGIKATHPAVLSVMVTALHDGPTVIDLINEGQLFRLIGKPVKPSVL
                     RLALGSALRKHEFLRNEPGALARHRPAEAPAVAERLQRRGLLKRLLGSVRHLFSFGFG
                     TRSAAS"
     gene            complement(3330..5222)
                     /locus_tag="PK144_14300"
     CDS             complement(3330..5222)
                     /locus_tag="PK144_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF66945.1"
                     /translation="MNALLRRFRIGTRLRAGFAAVLLLVLLMIATALGNLGGIQDNID
                     DLVHDNVRKMTLNTTMMNALNRIHSIMRDAAIKALNGDTAGALRRRDDVAGFRSAYDN
                     ARRELETLPASPQGRAIRERIDAATLAVRPYTNRAFELIQDGKTADALAVIDGPMNEG
                     LRSWRTALEDNLRRQETANRQVYEEAQAEYRSTQAWLLGIAALVLVLGIATAVLVTRS
                     IVLPLREAVGVLGAVAGGDLSKHAPVAGNDELTVLATALNQAIDAQRSALADIDAANR
                     EAQAAAAAQAEQERRLAAAEHEKREAERAQAEHERIEAARMQAEVAQIRAAVHEAAHG
                     DLRQQLDIPADRPTGEVADALNRLFADLRLSITPIGENAHTLAAASEELGAVSKQMNT
                     DAATNAEDAGRAALSAGQVSQRVENVAAATEEMSASIREISRATAEASNVTDEAVRVA
                     TDAQALIQHLGESSSHIGEVVKLISSIAEQTNLLALNATIEAARAGEAGKGFAVVAGE
                     VKELAKATAGATDEIAQRVTGIQTDTQGAIASIGRIFEIIRRVSDIQTSVAGAVEEQN
                     AVANDIAGNLAHTVQASGEIARGIDRVAQTASSTEQGSREIQQAAGQLAKMAAQLQEL
                     VGRFRC"
     gene            5458..5592
                     /locus_tag="PK144_14305"
     CDS             5458..5592
                     /locus_tag="PK144_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66946.1"
                     /translation="MKINKLLILINIKDIHAQFAAGVGEWAFRDTESAAGGERTPAIS
                     "
BASE COUNT          792 a         2089 c         1896 g          883 t
ORIGIN      
        1 aggcacggat tcatgccgct cccacgcagg aacggcaggc cgccgccacc gcatccatca
       61 ctggcttttc cgaccggctc ccgccggttc cagctgaagc gcagtacccg tatcgagccg
      121 ggccacgggg ccggtccgcg cttgcgaacg aatgccggca gatgccacgg gaggccacac
      181 catgccgcaa tcgccacgcc agtccgccac cgccggcatc gtcgccgccg gtgtcctgct
      241 cgtcgccgcc acggccgccg gcctgtgggc gatccagaaa aaacacgcca tcgacctgga
      301 ggcgacgcgc gccgcctaca tccaggaaag ccaccagcgc ctgctcgccc gtcgcgaaca
      361 ggtcgagatc gaactcggtg cgctggcgcg cggcctgcgc gcgatcgcgc gtctgccggc
      421 cgtgcaggcg cagggtccgg ccggagccgc cgaaccggaa accgccgcag caatcggcga
      481 gatcttcctc ggccagcagc gcctgcgtcc gcagcggctg ctggtggtcg gcaccggcag
      541 cgatgcgccg atgccgtacc ggctcgatgc cgtgaccgtg ctgccgtgga ccgccccgcg
      601 caaggccgcc agcgccgact gcacggcgcg cagcgacgcg ctgctcgacg aacgcctgcg
      661 cgggcagctc gcatacctgc agcagcagca cccgcgcgcc aacgccgtgc cgtggtcggc
      721 ctatccggcg atcggcgccc gggcggtgcc ggactgcctc gatccggacg gtgccggcca
      781 cttcctgtgg acggtgccgg tctacgccac cgatggcacg ctcagcggcg cggtcgccgt
      841 cgtgctgccg tcccgcgcgc tggccgccgc catcggcgcc ggtgccgagg acggcaactt
      901 cgcgctctac aacgccgatc acgattatct ggtcgccggc ggtcccggcg gcgaggccgg
      961 ccgtgctgcc ggctggatcc gccagcagcg cgccgccccc gacacgatct actccgaaac
     1021 catgccgctc ggcctcgccg gcgcggcgcg cggctggctg gtctggtacg gccgcccgga
     1081 cagcgactgg gccacgcgcc ccgacgtgct cggtgccgat cgcttccacc ggctggcgca
     1141 ggcggtcgtg atcggcctcg ccgtggtcgc agcgctcgca ctcgcgctcg gtgcccgccg
     1201 ccggcgcgtg ctcgaacgcc tggtcgccga acgcacgcgc gaactgcacg agagccaggc
     1261 ggcgctggtg caggccggtc gcctcgaagg cctcggcacg ctcgcggccg gcatcgccca
     1321 cgagatcaag aacccgctgg cctggacgcg cagcaacctc gatgtctgcg tcgatcacgc
     1381 ccgcacgctg gccagcctga gcaaggactg gcgcgaggcg cagcagcccg gggccgacac
     1441 cgatgcacgc gcgctgaaac tcgggcgcac gctgatcgcc gccgaaacct tcgtacgcga
     1501 ggaaacggcg accgagctga tgcagctgct cgaagactgc gccgacggca ccacgcgcat
     1561 ccaggccatc gtcgaggccg tcggcagctt cagccggctc gaccagcgtc aggtcgccag
     1621 caccgagatc ccgaagctgg tcgcgagcgt gctcagcatc gccggctcgg aaatccgcaa
     1681 gtgcggcgcc cggatcgaca cccgctacgc cgaagacctg ccggcgatca tctgcgcacc
     1741 cggccagctc gcgcaggtgc tgctgaatct ggtcgtcaac gcctgccacg ccatgcaggg
     1801 cgccgccgag caccggctga cgatcagcgc cgagctgcac gcgcggggcc ggcgcgtgcg
     1861 gctggtggtc agtgacaccg gctgcggcat cccggccgag atccgcgacc acatcttcga
     1921 tccgttcttc acgaccaagg acgtcggcaa gggcaccggc ctcggcctgc acctcgcgca
     1981 ccagatcgtg cgtgcccacg gcggacgcat cctcgtcgac agcacgccgg ggcacggcgc
     2041 gagcttcacg ctcgatctgc cggtcgatgc ctccgccgaa cttgccgcgg ccgctccggt
     2101 cgatgctgcg gccaccaccg ctgccgcccc ccgcccggcc ggcaatccgg acccgtccga
     2161 acctctgcgc ccgctgggcg cggctgcctg agggaccccc gatgagcacg acgccgcatc
     2221 tcgttcctgt tcccgtttcc ggcacccgcc cgcagcccgc cggcagcaag ctgccgaccg
     2281 tgctgttcgt cgatgacgaa ccaccgatca cgcgttcgct gtcggcgctg ttccggcgca
     2341 ccggccagta cgcggtgctg accgccaact ccgcccggga cgcgctggag ctgctcggca
     2401 cgcacgacat cgacgtgatc gtcagtgacc agcgcatgcc gggcatgacc ggcgtcgagc
     2461 tgctgcgcga gtttgcgacg cgctcgccgc gcacgatgcg catcctgctg accgcgtggt
     2521 cggacctctc ggcgatcact gccagcatca acgaaggcga agtgttccgc ttcgtgcaga
     2581 agccctgggg caacgacctg ctcgcgaccg tcgccgttgc cgcctccgcc tcgctggccc
     2641 cggccaacga accggtgcac ggcgctcccg atctggtgcc ggccgccgcc cagggcaccg
     2701 gcgtcggcat cctggtcgtc gacgatggcc gcgaactgct cgatctggtc cgggccgact
     2761 tcggcagcac gcatccggta catgtcgccc gccacgccga tcaggccatc gccctgctcg
     2821 aacagcacga gggcatcggc atcgtcgtct ccgacgtgcg cgtcgccgac gaggacatga
     2881 ccgatgtgat caagggcatc aaggccacgc acccggcggt gctgtcggtg atggtcacgg
     2941 cgctgcacga tggcccgacc gtcatcgacc tgatcaacga aggccagctg ttccggctga
     3001 tcggcaagcc ggtcaagcct tcggtgctgc gcctcgccct cggctcggcg ctgcgcaagc
     3061 acgagttcct gcgcaatgaa cccggtgccc tcgcccgcca ccgccccgcc gaggctccgg
     3121 ccgtcgccga acgcctgcag cgccgcggcc tgctgaagcg cctgctcggc agcgtgcgcc
     3181 acctgttctc gttcggcttc ggcacccgct cggccgcctc ctgaagccgc ggcatccgct
     3241 gccctgaaag ccatcgaccc cgtccggacg gggtcgatgg ctttctgcct gccggaaaac
     3301 gcgctgcgcg ttcctggacg acctccggct cagcaacgga agcgcccgac cagctcctgc
     3361 agctgcgcgg ccatcttcgc gagctggccg gcggcctgct ggatctcgcg gctgccctgc
     3421 tcggtgctgg acgcagtctg ggcgacgcgg tcgatgcccc gggcgatttc gccgctggcc
     3481 tgcaccgtgt gcgcgaggtt gccggcgatg tcgttggcga cagcgttctg ttcctcgacc
     3541 gcgccggcga cgctggtctg gatgtcgctg acgcggcgga tgatctcgaa gatgcggccg
     3601 atcgacgcga tcgcgccctg ggtgtcggtc tggatgccgg tgacgcgctg cgcgatctcg
     3661 tcggtggccc cggccgtcgc cttcgccagc tccttgacct cgccggccac gaccgcaaag
     3721 cccttgccgg cctcgccggc ccgcgcggcc tcgatggttg cgttgagcgc cagcaggttg
     3781 gtctgctcgg cgatcgagct gatcagcttc acgacctcgc cgatgtggct gctgctctcg
     3841 ccgaggtgct ggatcagcgc ctgcgcatcg gtggccacgc gcacggcctc gtcggtgacg
     3901 ttcgaagcct cggccgtcgc gcgcgagatt tcgcggatgc tcgcgctcat ctcctcggtc
     3961 gccgccgcga cgttctcgac ccgctggctg acctgaccgg cgctgagcgc ggcccggccg
     4021 gcatcctcgg cgttggtggc ggcatcggtg ttcatctgct tgctgaccgc cccgagttcc
     4081 tcgctggccg cggccagcgt gtgcgcgttc tcgccgatcg gcgtgatgct gaggcgcagg
     4141 tctgcgaaca gccggttcaa ggcatccgcg acctcgccgg tcggccggtc ggccgggatg
     4201 tcgagctgct ggcgcaggtc gccgtgcgcc gcttcgtgga cggccgcacg gatctgcgca
     4261 acctcggcct gcatgcgggc ggcctcgatg cgttcatgct ctgcctgcgc acgctcggct
     4321 tcgcgcttct cgtgttcggc cgcggccagc cggcgttcct gctcggcctg cgcagcagca
     4381 gcggcctggg cttcgcggtt ggcggcatcg atgtcggcga gtgcgctgcg ctgggcgtcg
     4441 atcgcctggt tcagcgccgt cgcgagcacc gtcagttcgt cgttgccggc caccggcgcg
     4501 tgcttcgaaa gatccccgcc ggcgacggca ccgagcacgc cgaccgcctc gcgcagcggc
     4561 agcacgatgc tgcgcgtgac cagcaccgcg gtggcgatgc cgagcaccag caccagcgcg
     4621 gcgatgccga gcagccatgc ctgcgtgctg cggtattcgg cctgggcttc ttcgtagacc
     4681 tggcggttgg cggtttcctg ccggcgcagg ttgtcctcga gcgccgtgcg ccagctgcgc
     4741 aggccttcgt tcatcggtcc gtcgatgacg gcgagcgcgt cggcggtctt gccgtcctgg
     4801 atcagctcga aggcgcggtt ggtgtacggg cgcacggcaa gggtcgcggc gtcgatgcgt
     4861 tcgcggatcg cacggccctg cgggctggcc ggcagggttt cgagttcgcg gcgggcgttg
     4921 tcgtaggcgc tgcggaaacc ggccacgtca tcgcgccggc gcagcgcgcc ggcagtgtcg
     4981 ccgttcagcg ccttgatcgc cgcgtcgcgc atgatcgaat ggatgcggtt cagcgcattc
     5041 atcatcgtcg tgttcagcgt catcttgcgg acgttgtcgt ggacgaggtc atcgatgttg
     5101 tcctggatgc cgccgagatt gccgagcgcg gtggcgatca tcagcaggac cagcagcagc
     5161 acggcggcaa acccggctct gagccgggtg ccgatacgga aacgacgcag cagggcgttc
     5221 atgacaagga ctccggcggg acggcggaag aaggatcagt cgggcttgcg cggattcccc
     5281 ccgcgtttct ggaatctgga tcacccccag ccaatgcgtc ggcagccggc ggtaaaactg
     5341 aaggcgggac tttactccca aatccctgct gaaccacgct cacctgcccc cattcgcgct
     5401 ggcgcccggc tttgcaagat ttgcaatcct gccgccgtcc caggtctacg taaaatcatg
     5461 aaaatcaaca agttattaat tttgattaat atcaaagaca tccatgcgca atttgcagca
     5521 ggggtcgggg aatgggcttt ccgggacacc gaatccgctg ccggcggtga gcgcacaccg
     5581 gcaatctcct gatcttcagc cctgccctcc agagcggccc gcggtcacag gccgacggcg
     5641 acgccgagct cgatcccgac
//
LOCUS       DAOQIE010000257         5636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_7975_length_5636_cov_8.194410, whole genome shotgun sequence.
ACCESSION   DAOQIE010000257 DAOQIE010000000
VERSION     DAOQIE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_5636_cov_8.194410"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1442)
                     /locus_tag="PK144_14310"
     CDS             complement(279..1442)
                     /locus_tag="PK144_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNF66947.1"
                     /translation="MKRFPVAPRADWQQTAADCGFRFHTMYGEPYWDESACYAFTLEE
                     IERDLEGPSAELAMMCLDVVDIAVRDEAILHRLGIPAGQHDFVHDAWRRGEASLYGRF
                     DFAYDGRGPARLYEYNADTPTALYETAYFQWVWLEQALARGLIPAGCDQFNSVQEVLI
                     ERLSRMAPPEGPMHFTCARDSEEDRGTVEYLRDCAEQAGIPTRFLFVDEIGVDSNGQY
                     TGLDGEALRHLFKLYPWEFLFREDFARFLPGCDTYFIEPPWKAVLSNKGLLALLWEHF
                     PGHPNLLPTWFADDPRANRLGASYVRKPLYSREGANVRIVERGRIVCDEPGPYGAEGH
                     VLQALCPPPRFAGRHVVIGSWIIGHEACGIGVREDSGPVTKDLSRFVPHVILN"
     gene            complement(1439..2071)
                     /locus_tag="PK144_14315"
     CDS             complement(1439..2071)
                     /locus_tag="PK144_14315"
                     /inference="COORDINATES: protein motif:HMM:NF018408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HNF66948.1"
                     /translation="MKRSKMLKLVLMGAAGTLAAGCGDDADDGYDISEDVTVYKTVQE
                     CVDKGVFTENFCRENFDKAKELHEKVAPRFTAQSSCEAQFGPGGCEARQTDTGSIWLP
                     AIAGFMLGQALGNRRSEVIYQPLYNSYSSSGSYGGGGGGGYSYGSYRTWNSPAPIRPN
                     ASGSARFDTDVLRGAASAQKPAFARTTTVARGGFGARASSGSSSSSGFGG"
     gene            complement(2089..2490)
                     /locus_tag="PK144_14320"
     CDS             complement(2089..2490)
                     /locus_tag="PK144_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNF66949.1"
                     /translation="MNYLVGLPAFALYFVLSSALLAAYCAAYTRLTRHDEMALIRAGC
                     TPAAIAYTGSILGFALPLASAVAHSVALLDCLLWGLIALGVQCSVYLGLRRWCMPDIS
                     ERIERGETAAGTLLAGASLATGLLNAASMSY"
     gene            complement(2560..3255)
                     /locus_tag="PK144_14325"
     CDS             complement(2560..3255)
                     /locus_tag="PK144_14325"
                     /inference="COORDINATES: protein motif:HMM:NF022144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2491 family protein"
                     /protein_id="HNF66950.1"
                     /translation="MGLLDSLFKLGKSRVREAVSGRKPERTLPSALGLRIGASVEFDL
                     LPVRMNAGAFRFALPRTDEPMIVTAQGRFELGEGMIVHRFYSDSSTMLQLLCRDLPGL
                     EDQVEEITLYVPLESAYPENREQIHRWVGPQGRIGAPEFRIADGTRYRRIWFDDEPGP
                     ASPVRYTEEVFDEPDSFSRPRRIAQEAMLYGREVAPGRSEYLLPALETTDGESSVTVM
                     VGIDLDRSAVRVL"
     gene            3434..4066
                     /locus_tag="PK144_14330"
     CDS             3434..4066
                     /locus_tag="PK144_14330"
                     /inference="COORDINATES: protein motif:HMM:NF021457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HNF66951.1"
                     /translation="MEEQKKTSADYQRAFRERMRAAGYVKRDVWVPPEQSEALRACEL
                     ALRKGIVPIIVKRRRAGMGNTTNSFVWTTETLLAALTDTDAVRAGRLKVESIEGLEPC
                     IGIRFPELEDLPVFMIAGGTQLLVSTLLWRVDEVADPADFNKLLLRTHKILPLSTFGI
                     VTMPDGEYYELFGALSAASDLEEILEEIETLGDNVVQVYESFGSYLKRAD"
     gene            4103..4840
                     /locus_tag="PK144_14335"
     CDS             4103..4840
                     /locus_tag="PK144_14335"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNF66952.1"
                     /translation="MGIFSKIITAIRGGATEVGEAIVDSQAMRILDQEIRDAGEDLRL
                     AKDELTKLMAKRKLADEDVRALGSKVGEYEGYLGQVLAREAPDGSLAAQDAQLRDELA
                     AKIAELEGELGQKQSLAQEFAQRENSVKTAIRSAEGNLDKLKQRASMVKATDAVHKAQ
                     AQIASSHSGANARMTTAMDSLARLEQRQKEQSARFDAATQLEHETQSSERSLEDKLKA
                     SGLIGSGPSSSDVLARLRAKRLPDHSS"
     gene            4980..>5636
                     /locus_tag="PK144_14340"
     CDS             4980..>5636
                     /locus_tag="PK144_14340"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNF66953.1"
                     /translation="MTVPSYKRPQILALAPDPASAKAGEGLARRAKWLHYATDGRALW
                     GECQGSGSTPYRTQVDLAGPAFRCSCPSRKFPCKHGLGLMLLHADVPAASAETPDWVG
                     EWLAKRESRAATAPAAPATPAEAAPAPAAKATADPEAAAKRAEKRLARVSAGVEALGL
                     WLADAVRTGLDGAPQQPPGHWREAAARLDDAQAPGLARRVRSLAALVAAGDGWEPRLL
                     D"
BASE COUNT          903 a         1890 c         2014 g          829 t
ORIGIN      
        1 attccatagg gccaatagcg gagaggtgtc tggtggcgag cctcgggctg cggaggaagt
       61 ccggatatag cggggcgggt gtaaatgtca atgtcggccg gaatttcctg gaattgtcgg
      121 ggttattgct aagcccggcg atcaggcatg cccatccgcc tcgatcagcc cggtcgcaaa
      181 gacgcgtcat cggcgcgatg cctgcgtggc gtttgttcag ccgagcatga ctgcgccgcg
      241 gacgggtgcg catcgaaatg actcggatgc gctccggatc agttcaggat cacgtgcggc
      301 acgaagcgcg acaggtcttt cgtgaccggg ccggagtcct cgcgtacgcc gatgccgcag
      361 gcttcgtggc cgatgatcca gctgccgatg acgacgtgcc ggccggcgaa gcgcggcggc
      421 gggcacaggg cctgcagcac gtggccttcg gcgccgtagg ggccgggttc gtcgcagacg
      481 atgcggccgc gttcgacgat gcgcacgttc gcgccctcgc gcgagtacag gggcttgcgc
      541 acgtagctgg cgccgaggcg gttggcgcgc gggtcgtcgg cgaaccaggt cggcagcagg
      601 ttcgggtgac cggggaagtg ctcccacagc agcgccagca ggcccttgtt cgacagcacc
      661 gccttccacg gcggttcgat gaaatacgta tcgcatccgg gcaggaagcg ggcgaaatcc
      721 tcgcggaaca ggaattccca cgggtagagc ttgaacaggt ggcgcagcgc ctcgccatcg
      781 agcccggtgt actggccgtt gctgtcgacg ccgatctcgt cgacgaacag gaagcgcgtc
      841 gggatgccgg cctgctcggc gcagtcgcgc aggtactcga cggtgccgcg gtcctcctcg
      901 ctgtcgcgcg cgcaggtgaa gtgcatcgga ccctcgggcg gggccatgcg cgacaggcgc
      961 tcgatcagca cctcctgcac gctgttgaac tggtcgcagc cggccgggat cagcccgcgt
     1021 gccagtgcct gttcgagcca gacccactgg aaatacgcgg tttcgtacag cgcggtcggc
     1081 gtgtcggcgt tgtactcgta gagccgggcc gggccgcggc cgtcgtaggc gaagtcgaag
     1141 cgcccgtaca gcgaggcttc gccgcgccgc caggcgtcgt gcacgaagtc gtgctgtccg
     1201 gccgggatgc ccagccggtg caggatggcc tcgtcacgca cggcgatgtc gaccacatcg
     1261 agacacatca tcgccagttc ggcgctcggt ccttcgaggt cgcgctcgat ctcctcaagc
     1321 gtgaaggcgt agcaggccga ctcgtcccag tagggttcgc cgtacatggt gtggaagcgg
     1381 aagccgcagt cggcggccgt ctgctgccag tcggcgcgcg gcgcgaccgg gaagcgcttc
     1441 atccgccgaa gcccgacgac gaactggaac cggagctggc gcgcgcaccg aagccgccgc
     1501 gtgcgaccgt ggtcgtgcgc gcgaacgccg gcttctgcgc actggccgca ccgcgcagca
     1561 cgtcggtgtc gaagcgcgcc gagccggagg cgttcgggcg gatcggtgcc gggctgttcc
     1621 aggtgcggta gctgccgtag ctgtagccgc caccgccacc gccaccgtag gaaccgctgg
     1681 acgaatagct gttgtagagc ggctggtaga tgacctcgga gcggcggttg ccgagcgcct
     1741 ggccgagcat gaagccggcg atggccggca gccagatgct gccggtgtcg gtctggcgcg
     1801 cctcgcagcc gccggggccg aactgggcct cgcatgagga ctgcgccgtg aagcgcggcg
     1861 cgaccttctc gtgcagttcc ttcgccttgt cgaagttctc gcggcagaag ttttcggtga
     1921 acacgccctt gtccacgcac tcctgcacgg tcttgtagac ggtgacatcc tcgctgatgt
     1981 cgtagccatc gtcggcatcg tcgccgcagc cggcggcgag cgtgccggcg gcgcccatca
     2041 gcacgagttt cagcatcttc gagcgtttca tggccggtcc cgcccctttc agtagctcat
     2101 gctcgcggcg ttcagcaggc cggtcgcgag cgaggcgccc gcgagcagcg tgccggcggc
     2161 agtctcgccg cgttcgatgc gctcggagat gtccggcatg caccagcggc gcaggccgag
     2221 atagacgctg cactgcacgc cgagcgcgat caggccccac agcaggcagt cgagcagcgc
     2281 caccgagtgc gcgacggcgc tggcgagcgg cagcgcgaag ccgaggatcg agccggtgta
     2341 ggcgatcgcc gccggcgtgc agccggcacg gatcagcgcc atttcgtcgt ggcgggtcag
     2401 gcgggtgtac gcggcgcagt aggcggcgag cagcgcgctg ctcagcacga agtacagcgc
     2461 gaaggcgggc aggccgacga gatagttcat ggcgggcagg aagttccggt ggaaaaggtc
     2521 tgtcggacgg ccgcggcgcg gcggccgggc ggagcgggtt cagagcaccc gcacggcgct
     2581 gcggtcgagg tcgatgccga ccatcaccgt caccgaggat tcgccgtcgg tggtctcgag
     2641 cgccggcagc aggtattcgc tgcgccccgg cgcgacctcg cggccgtaga gcatggcctc
     2701 ctgcgcgatg cgccgcgggc ggctgaagct gtcgggctcg tcgaagactt cctcggtgta
     2761 gcgcaccggc gaggccgggc cgggctcgtc gtcgaaccag atgcgccggt agcgcgtgcc
     2821 gtcggcgatg cggaactccg gcgcgccgat gcgcccctgc ggcccgaccc agcggtggat
     2881 ctgctcgcgg ttctcggggt aggcgctctc cagcggcacg tacagcgtga tctcctcgac
     2941 ctggtcctcc agaccgggca ggtcgcggca gagcagttgc agcatcgtcg aactgtcgct
     3001 gtagaagcgg tgcacgatca tgccttcgcc gagttcgaag cggccctgcg cggtgacgat
     3061 catcggttcg tcggtgcgcg gcagcgcgaa gcggaaggcg ccggcgttca tgcgcaccgg
     3121 cagcaggtcg aactcgaccg aggcgccgat gcgcagaccg agcgccgaag gcagcgtgcg
     3181 ctcgggcttg cggccgctga cggcttcgcg cacgcggctc ttgccgagct tgaacaggga
     3241 atcgagcagg cccatgacaa cattcacccc ggggttttcg cgcgcggtgc gcgcccgcac
     3301 tatagctttc cgtgccggct gtctccggca gcgcacggtc tgacccggct acgatagcag
     3361 ccgttgccgg tgctacgatc cttacaggtg aggctgcagc ctcgccgcac gcagtcggcg
     3421 gaccttcagc acgatggaag aacagaagaa aacctcggcg gactaccagc gcgcgttccg
     3481 cgaacgcatg cgcgccgccg gctacgtgaa gcgcgatgtc tgggtgccgc cggagcagag
     3541 cgaggcgctg cgcgcctgcg agctggcgtt gcgcaagggc atcgttccga tcatcgtcaa
     3601 gcgcaggagg gcggggatgg gtaacaccac gaattcattc gtctggacca cggagacgct
     3661 gctggcggcc ctgaccgaca ccgacgcggt gcgcgccggc cggctgaagg tcgagagcat
     3721 cgaggggctg gagccgtgca tcggcatccg cttcccggaa ctggaagacc tgccggtgtt
     3781 catgatcgcc ggcggcacgc aactgctggt cagcacgctg ctctggcgcg tcgacgaggt
     3841 cgccgatccg gccgatttca acaagctgct gctgcgcacg cacaagattt tgccgctgtc
     3901 gaccttcggc atcgtcacga tgcctgacgg cgagtactac gagctgttcg gcgcgctgag
     3961 cgcggcatcg gatctcgagg aaatcctcga agaaatcgaa accctcggcg acaacgtcgt
     4021 gcaggtctac gaatccttcg gctcctacct gaagcgcgcc gactgaccga ctgacagact
     4081 tattccggag gtccggacat ccatgggcat cttcagcaag atcatcaccg cgatccgcgg
     4141 cggtgccacc gaggtcggcg aggcgatcgt cgacagtcag gcgatgcgca tcctcgatca
     4201 ggaaatccgc gacgccggcg aggatctgcg cctggccaag gacgaactga ccaagctgat
     4261 ggccaagcgc aagctcgccg atgaggacgt gcgcgcgctc ggcagcaagg tcggcgaata
     4321 cgagggctat ctcggccagg tgctggcgcg cgaggcgccg gacggctcgc tggcagcgca
     4381 ggatgcgcag cttcgcgacg aactcgcggc caagatcgcc gaactcgaag gcgagctcgg
     4441 ccagaagcag tcgctggcgc aggagttcgc gcagcgcgag aacagcgtca agaccgcgat
     4501 ccgcagcgcc gagggcaacc tcgacaagct caagcagcgc gcctcgatgg tcaaggcgac
     4561 cgacgccgtg cacaaggcgc aggcgcagat cgcgtcctcg cactccggag ccaacgcgcg
     4621 catgaccacg gcgatggatt cgctggcccg cctcgaacag cgccagaagg agcagtcggc
     4681 gcgcttcgac gccgccacgc agctcgaaca cgagacgcag agcagcgagc gttcgctgga
     4741 ggacaagctc aaggcttcgg gcctgatcgg cagcggtccg agcagctccg acgtgctcgc
     4801 gcgcctgcgc gccaaacgtc tgccggatca ttcgtcctga gcccgccggg cggcggcacg
     4861 tgcgccgggc cggcgtgccg cctcgggtgg ccgcccggat ttcgtgaggc gcccgggctg
     4921 cagaccggcc gttcatcgtc catgctgccg gccatccttc ccgccatgcc ggtagcctct
     4981 tgacggtccc gtcgtacaag cgcccgcaga tcctcgccct cgcccccgac ccggcttccg
     5041 cgaaagccgg cgaagggctg gcgcgacgtg cgaaatggct gcactacgcc accgacggcc
     5101 gcgcgctgtg gggcgaatgc cagggcagcg gcagcacgcc gtaccgcacc caggtcgatc
     5161 tcgccgggcc ggccttccgc tgctcgtgcc cgagccgcaa gttcccgtgc aagcacggcc
     5221 tcggcctgat gctgctgcac gccgacgtgc cggccgcgag tgccgagacc ccggactggg
     5281 tcggcgaatg gctcgcgaag cgcgaaagcc gcgcggcgac agccccggca gctccggcaa
     5341 ctcctgccga ggctgcgccg gccccggccg cgaaagcgac ggccgatccc gaggccgcgg
     5401 cgaagcgcgc cgaaaaacgg ctcgcgcgcg tcagcgccgg cgtcgaggcg ctcgggctgt
     5461 ggctcgccga tgccgtgcgc accggcctcg acggcgcacc gcagcaaccg cccggccact
     5521 ggcgcgaggc cgcggcgcgc ctcgacgatg cgcaggctcc gggcctcgcg cggcgcgtgc
     5581 gctcgctcgc ggcgctggtg gcggccggcg acggctggga gccgcgcctg ctcgat
//
LOCUS       DAOQIE010000258         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8012_length_5619_cov_6.756650, whole genome shotgun sequence.
ACCESSION   DAOQIE010000258 DAOQIE010000000
VERSION     DAOQIE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8012_length_5619_cov_6.756650"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..1137)
                     /gene="ttcA"
                     /locus_tag="PK144_14345"
                     /pseudo
     CDS             complement(313..1137)
                     /gene="ttcA"
                     /locus_tag="PK144_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004089842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            1304..1732
                     /locus_tag="PK144_14350"
     CDS             1304..1732
                     /locus_tag="PK144_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNF66954.1"
                     /translation="MPSTYEAGPDGRGWHAVDERPRAAADPVLEARLRELRQLAWLLD
                     ARFRIPGTGWRIGLDGLIGVIPGIGDGLPMLAGAWIIWRSAQLGVPQTTLLRMVGNLL
                     IDAAAGSVPVLGDVFDVAFKANLRNLDLLHEHFGLPPMRR"
     gene            1904..2236
                     /locus_tag="PK144_14355"
     CDS             1904..2236
                     /locus_tag="PK144_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66955.1"
                     /translation="MSQTLDLQPGLYFTIAPDKPDAAPAGLPPHLSRHIAYLALGLGM
                     HPASGEPCIVLSNDRDELCTVPSRLLRTYRLIPGSRCLRMVLFPPHVPELNPQLRFQP
                     LPAEPAHA"
     gene            2305..2547
                     /locus_tag="PK144_14360"
     CDS             2305..2547
                     /locus_tag="PK144_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HNF66956.1"
                     /translation="MPQGDKGKYTDKQKRQAAHIEQGYEDRGLPEDEARRRAWATVNA
                     MDHGGKKPGGGGRGKPVNRAPARKGGHNSHAHGRHH"
     gene            complement(2572..3828)
                     /locus_tag="PK144_14365"
     CDS             complement(2572..3828)
                     /locus_tag="PK144_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNF66957.1"
                     /translation="MTDSTVAVIGAGPAGLMAAEVLATAGCTVEVHEAMPSPARKLLR
                     AGVGGLNLTHGEPPAQFLSRYGDRQPEIAPLLAGFDAAALRAWVHGLGIDTFVGSSGR
                     VFPVGMKAAPLLRAWLQRLHGLGVRLHLRHRWLGWSGDGALRIRTADGGEQCLRPAAT
                     VLALGGASWPRLGADGAWAQWLAARGIALAPFAPANCGFDVAWSETFRTRFAGAPLKT
                     VELQYIDAAGHRWQRRGDAVITAHGIEGGLIYALSAPLRDGLANGGRAVPTLDLLPDR
                     SLDKVTAALAKPRGRRTHAAHWQRTLGLEGVRAGLLRERMTPELLDDAPALARRIKAL
                     PLALVAPRPLAEAISTAGGVCFGALDEQLMLRALPGTFCAGEMLDWEAPTGGYLLTAC
                     LASGRAAGLGVLAWLQQTGRRKDAPA"
     gene            3992..4534
                     /locus_tag="PK144_14370"
     CDS             3992..4534
                     /locus_tag="PK144_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66958.1"
                     /translation="MNLKLTKPKLFAERSLSKVAGVFDDADTARVAARRARNEGNMVL
                     PQVQIVAPGDPQLSARIEPEQERIPETLLRSHLVCGTAGAGLGLLLAFALALSGVAAV
                     TSNLTASLMLLPLFGGLFGLLIGGAIALRPDIGRVAMTVRKETQSGRWVVVAHPVNAD
                     QQHGAERVFQDLRARTVSSL"
     gene            complement(4584..5564)
                     /locus_tag="PK144_14375"
     CDS             complement(4584..5564)
                     /locus_tag="PK144_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNF66959.1"
                     /translation="MPIPSYLLTGLLGSGKTTVLRALLAQAPAGSRPAVLVNDFGTVG
                     IDAAVLAGAGGAGEITVREVGGGCVCCTAAASLRAAIPALLRATRPDRLYIEPSGLAA
                     PAAIVDLLREPALRGAIELRAVIGLVDPRRLDDERLLRAPLLRAQLELADVLLATHAD
                     VASPAQLERFRAFASTLYPPRAHIGAIAHGDFDPQWLKDITRAAQPGWRLTRSQPPAA
                     RAEHGWTFAPEVIFAAPKLTALFAGIGRDGPLAPVERAKGVLRTGRDWRLFDAVAGGG
                     LTVSDVSWRRDSRIECIAFEDARPDWTAIGAAWLAARGEAGAEGGQQGGQ"
BASE COUNT          826 a         2092 c         1939 g          762 t
ORIGIN      
        1 gggggagcgg aatcggacat ggagacgggc acgcacgggg ggcgccgtgc agttcagcgc
       61 agcggcgtaa cacgcgccgc gccgggcacg gcggctttgt aacggcttgt agacgagacg
      121 gcggcttcag ccggccccga tcgggatgcc gcggaacatg cgcacgccgc ccggcccgcc
      181 ggcatcgccg ctgccggcgc cgatgcgcgg ctcgccgccg tcgccatcgc cgccgtcggc
      241 ggcggccgga tcgtcccgat cctcaggggc gtcgtcgcca gtttcccgcg ccggcaccag
      301 ccagtcgcgg cgcgctgcgg ccgtggcacc gagaccggcg aagtcgaaca gcccggcatc
      361 ggcgagctgc gagggcgcga cgttcttcac ggccgagaac atgctctcca gccgccccgg
      421 atggcgcctg tcccagtcgc gcagcatgtc gccgatcacc gcgcgctgca ggttcggctg
      481 cgagccgcac agattgcacg ggatgatcgg gaagcccttc gcggccgcgt agcgctcgat
      541 gtccttctcc ttgcagtacg cgagcggccg gatcacgacg ttgcggccgt cgtcggacct
      601 gagcttcggc ggcatcgcct tcaggctgcc gccgtagaac aggttcagca gcagggtttc
      661 gaggatgtcg tcgcggtggt ggccgagcgc gatcttgctg aagccgtgcg tgtgcgcgta
      721 ggtgtacaga atcccgcgcc gcagccgcga gcacagcgag cacgtcgtct tgccctcggg
      781 cacgacgcgc tgcacgacgc tgtaggtgtc ctgctcgatg atctcgaacg gctggccgat
      841 cgaggcgagg taatccggca gcacgttcgc cggaaagccc ggctgcttct ggtcgaggtt
      901 gacggcgaac agctcgaagc gcaccggcgc cgactgctgc agcgagcgca ggatgtcgag
      961 catcgtgtac gaatccttgc cgccggacag gcagaccagc acgcggtcgc cgtcctcgat
     1021 catgttgtag tcctggatcg cctcgccgac gccgcggcgc aggcgcttgg cgagcttgac
     1081 gaggttcacg cgctcgcgtt cggagcgcgg ggacggcacg gcggctggct ggttcatcac
     1141 gaacggtccg gaagtggcgc gcgacccgca cccgggccgc gaaaggcgcg gaatgctagc
     1201 aggcacacgg gcttgtcggt atcgcgccgg ctgcttccgg aacaccggcg gccggaagca
     1261 gtcgataacc ggaccctcac ctgtaatgaa cggagcctcc tccatgccga gcacctacga
     1321 agccggaccc gatggccgcg gctggcacgc cgtcgacgaa cgtcctcgcg ccgccgccga
     1381 tcccgtgctc gaagcccggc tgcgcgagct gcgtcagctc gcctggctgc tcgatgcgcg
     1441 cttccgcatc cccggcaccg gctggcgcat cggcctcgac ggactgatcg gcgtcatccc
     1501 cggcatcggt gacggcttgc cgatgctcgc cggggcatgg atcatttggc gcagtgcaca
     1561 actcggggtg ccgcagacga cgctgctgcg catggtcggc aacctgctga tcgacgccgc
     1621 cgccggcagc gtgccggtgc tcggcgatgt cttcgacgtc gcgttcaagg ccaatctgcg
     1681 caacctcgac ctgctgcacg aacacttcgg cctgcccccg atgcgccgct gaatcctgcc
     1741 ggaacaggcc ggatccgacc ggctcccgcg ggattcaggc cgctgaattt tcggacaggc
     1801 gggaaccccg tcgttgacgg gtctgccaca atccgggccg caactgccat cagcgtgtcg
     1861 caccccgcag gacacgcctt cacgaacgcc gcgagaaacc gccatgagcc agacgctgga
     1921 cttgcagccg ggcctgtatt tcacgatcgc cccggacaag ccggacgccg cccccgccgg
     1981 actgccgccg catctgtccc ggcacatcgc ctatctggcg ctcggcctgg gcatgcatcc
     2041 ggcgagcggc gagccctgca tcgtgctgtc gaacgaccgc gacgaactct gcacggtccc
     2101 gagccggctg ctgcgcacct accggctgat ccccggctcg cgctgcctgc gcatggtgct
     2161 gttcccgccg cacgtgcccg aactcaatcc gcagctgcgt ttccagccgc tgcccgccga
     2221 accggcccac gcctgacacg cagcggacaa ccgcatgaca ggcgcgcggg ccggtttcct
     2281 tccctgacga ccgaggaggc ccccatgccc caaggtgaca aaggcaagta caccgacaag
     2341 cagaagcgcc aggccgccca catcgagcaa ggctacgaag accgcggcct gcccgaggac
     2401 gaagcccgcc gccgcgcctg ggcgaccgtc aatgcgatgg accacggcgg caaaaagccc
     2461 gggggaggcg gtcgcggcaa gcccgtcaac cgcgccccgg cccgcaaggg cggccacaac
     2521 agccacgccc acggccggca tcactgatcc tttccggcgc ccgccgtttc gtcaggcggg
     2581 cgcatccttc ctccgccccg tctgctgcag ccacgccagc accccgagcc ccgcggcccg
     2641 gccgctggcc aggcaggcgg tcagcagata gccgccggtc ggtgcctccc agtcgagcat
     2701 ctcgccggcg cagaaggtgc cgggcagcgc gcgcagcatc agttgctcat cgagcgcccc
     2761 gaagcacacg ccgccggccg tgctgatcgc ctcggcgagc ggtcgcggcg cgacgagcgc
     2821 gagcggcagc gccttgatcc gccgcgccag cgccggcgca tcgtcaagca gctccggcgt
     2881 catccgttcg cgcagcagcc cggcgcgcac gccttccagc ccgagcgtgc gctgccagtg
     2941 cgcagcatgc gtgcgccggc cgcggggctt cgcgagtgcg gccgtcacct tgtccagtga
     3001 acgatccggc agcaggtcga gcgtcggcac ggcccggccc ccgttcgcaa gtccgtcgcg
     3061 cagcggagcc gacagggcat agatcagccc gccctcgatg ccgtgcgcgg tgatcacggc
     3121 atcgccgcgc cgctgccagc gatggccggc ggcatcgatg tactgcagtt cgacggtctt
     3181 cagcggcgcc ccggcaaagc gcgtgcggaa ggtctcgctc caggccacgt cgaagccgca
     3241 gttggccggt gcgaacggcg cgagcgcgat gccgcgcgcg gcgagccact gcgcccacgc
     3301 gccatccgca ccgagccgcg gccagctcgc gccgccgagc gcgagcacgg tcgcggccgg
     3361 gcgcaggcac tgttcaccgc cgtcagcggt cctgatgcgc agtgccccgt cgccgctcca
     3421 gcccagccag cgatggcgca ggtgcaggcg cacgccgagc ccgtgcaggc gctgcagcca
     3481 ggcgcgcagc agcggcgcgg ccttcatgcc gaccggaaac acccggcccg aactgccgac
     3541 gaaggtgtcg atgccgagtc cgtgcaccca ggcccgcagc gccgcggcat cgaagccggc
     3601 gagcagcggc gcgatttcgg gctggcgatc gccgtagcgc gacaggaact gcgccggcgg
     3661 ctcgccgtgc gtcaggttca ggccgccgac cccggcgcgc agcagcttgc gcgccggcga
     3721 cggcatcgcc tcgtggacct cgaccgtgca gccggccgtc gccagcacct cggccgccat
     3781 cagtccggcc ggcccggccc cgatcacggc caccgtcgaa tccgtcaagt cccgccctcc
     3841 tccggcgtct gcgtccggcc attctgcagc ttcgtgccag ccttgctgaa ccttgcggaa
     3901 ctgtcatatg cccggccggt cgcaatggcg gggtcgaagg ccgcttccgc atgaggcggg
     3961 agcttcatgg aaccgatcaa ggagaaagcc gatgaacctc aagctgacga aaccgaagct
     4021 gtttgccgag cgttcgctga gcaaggtggc cggcgtcttt gacgatgccg acaccgcgcg
     4081 cgtcgccgcc cggcgtgccc gtaacgaagg caacatggtg ctgccgcagg tgcagatcgt
     4141 cgctcccggc gatccgcagc tgagcgcccg catcgaaccc gagcaggagc gcatccccga
     4201 aaccctgctg cgctcgcacc tggtgtgcgg cacggccggg gcggggctgg ggctgctgct
     4261 cgcgttcgcg ctggcgctgt ccggcgtggc ggccgtcacg tccaacctga cggccagcct
     4321 gatgctgctg ccgctgttcg gtggcctgtt cgggctgctg atcggcggcg cgatcgcctt
     4381 gcggcccgac atcggtcgcg tggcgatgac cgtgcgcaag gaaacccagt cgggccgctg
     4441 ggtggtcgtt gcgcatccgg tcaacgcgga ccagcagcat ggcgccgagc gcgtgttcca
     4501 ggacctgcgg gcgcggacgg tcagttcgct ctgatccgga ccgtttccag gcgcagccgg
     4561 ctcgatcgcc cggctgcgcc ttttcattgc ccgccctgtt gtccgccctc agcgcccgcc
     4621 tccccccgcg ccgccagcca ggccgcgccg atcgccgtcc agtccgggcg cgcgtcctcg
     4681 aaggcgatgc attcgatgcg gctgtcgcgg cgccagctca cgtcgctgac cgtcaggccg
     4741 ccgccggcga ccgcatcgaa cagccgccag tcgcgcccgg tgcgcagcac gcccttggcg
     4801 cgctcgaccg gggccagcgg accgtcacga ccgatgccgg cgaacagtgc cgtgagcttc
     4861 ggtgccgcaa agatcacctc gggcgcaaac gtccagccgt gctcggcgcg cgcggcgggc
     4921 ggctgcgatc gcgtcagccg ccagcccggc tgcgcggccc gcgtgatgtc cttcagccac
     4981 tgcggatcga aatccccgtg cgcgatggcg ccgatgtgcg cccggggcgg atacagcgtc
     5041 gatgcgaagg cgcggaagcg ttcgagctgc gcggggcttg cgacatcggc gtgcgtcgcc
     5101 agcagcacgt cggcgagttc gagctgcgca cgcagcagcg gcgcgcgcag caggcgctcg
     5161 tcgtcgaggc ggcgcggatc gacgaggccg atcacggcgc gcaactcgat cgccccgcgc
     5221 agcgccggtt cgcgcagcag gtcgacgatc gccgccgggg ccgccagccc gctcggctcg
     5281 atgtagagcc gatccggccg ggtcgcgcgc agcagcgccg gaatcgccgc gcgcaggctc
     5341 gccgccgccg tgcagcacac gcagccgccg ccgacctcgc gcaccgtgat ctccccggcg
     5401 cccccggcgc cggcgagcac cgcggcatcg atgccgaccg tgccgaagtc attgaccagc
     5461 accgccggac ggctgccggc cggtgcctgc gcgagcagcg cgcgcagcac cgtggtcttg
     5521 ccgctgccga gcaggccggt cagcaggtat gaggggatcg gcatctgggc atccgcaagc
     5581 aggggcggca ttctgtcggc aacgactgcc cgtcagagc
//
LOCUS       DAOQIE010000259         5615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8019_length_5615_cov_6.965288, whole genome shotgun sequence.
ACCESSION   DAOQIE010000259 DAOQIE010000000
VERSION     DAOQIE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_5615_cov_6.965288"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PK144_14380"
     CDS             complement(<1..585)
                     /locus_tag="PK144_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66960.1"
                     /translation="MPPLLSFLLRFLLSPGLMLALALPASAFDATDVPEPLRPWIGWV
                     RPADAPAGRCALAGGPGDWRCVWAGRLRIELAADGALFSGTWRLDREDWLLLPGDAAL
                     RPRAVGADGEPALVLLHDGHPALRLPAGTHKISGRLFWGDEAPAVLPLPPGSGLIELV
                     DAAGRPLAVTRTGDGLQLAAGADDGAREADHLELD"
     gene            817..1449
                     /locus_tag="PK144_14385"
     CDS             817..1449
                     /locus_tag="PK144_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNF66961.1"
                     /translation="MTTMDAAPTTAPDEDDRLRAGTWALLAALLRRPPTAELLAQLAA
                     IDTPPAGEGGALAAAWQDLRAAAAVADPVALEREYHALFIGIGRGELLPFASWYLTGF
                     LMEKPLGELRADLARLGFERQPGVCEPEDHIAALAEVLGLLIGEGSAAAAGFFAHHVE
                     PWAGRLFADLQTAESARFYRAAGRFAEAFLAFERQYGAMPALAPSSVPAG"
     gene            1529..1744
                     /locus_tag="PK144_14390"
     CDS             1529..1744
                     /locus_tag="PK144_14390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNF66962.1"
                     /translation="MSEKTPAAGLDRRAFLKLVGSGAGAGAVALALPGVAVAAPAAVR
                     PEPEEAGRLDAGYRETAHVRAYYDTCR"
     gene            1755..4658
                     /locus_tag="PK144_14395"
     CDS             1755..4658
                     /locus_tag="PK144_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNF66963.1"
                     /translation="MKLVRKQAGARPAAAATSPVAAFLGATVDRRAFLKSSGLALGAT
                     ALGAALGPTMMRRASAATDAGTHASNAAVEIKRTICSHCSVGCGIYAEVQNGVWVGQE
                     PAFDHPFNMGAHCAKGASIREHGHGERRLKYPMKLVDGQWQRISWDTALSEVSARLLK
                     IREESGPDAVYWLGSAKHSNEQAYLFRKFASMWGTNNVDHQARICHSTTVAGVANTWG
                     YGAMTNSYNDIAYAKSILMIGSNAAEAHPVSMQHILRAKEDGAKLIVIDPRFTRTAAH
                     ADHYVRIRPGTDVPFIWGLLWHVFQNNWQDDSFLQQRVFGMDEVRKEVEKWPPAEVER
                     VTGVQEKDVLAVAKLMAEHRPGTFVWCMGGTQHTIGNNNTRAYCIFQLALGNMGKAGG
                     GTNIFRGHDNVQGATDFGVLADSLPGYYGLAEGSWKHWAKVWGVDYEWLKARFDPNAY
                     DGGSGKPVKPMETSGIPVSRWIDGVLEDKALMTQRDNVRGMVFWGHAPNSQTRGTEMK
                     KAFEKLDTLVIVDPYPTVSAVMHERRDGVYLLPAATQFETYGSVTASNRSVQWREQVI
                     APLFESRPDHTILYQLAKKFGYADELCKNVKVTNDEPSIEDLVREYNRGMWTIGYTGQ
                     SPERLKAHQKNWHTFNTRTLRAEGGPLDREFYGLPWPCWGTPEMKHPGTANLYDTSLP
                     VADGGLCFRANFGVERNGVNLLAEHSYPAGSEIEDGYPEFDDKLVKKLGWWDDLTDAE
                     KTAAEGKNWKTDLSGGIQRVVIKHGCAPFGNAKARCVVWNFPDPVPIHREPLYTSRRD
                     LVGQYPTYADRKAYWRLPTRYASIQQQDFSQDFPIILTSGRLVEYEGGGDETRSNPWL
                     AELQQHMFVELNPKDANDAGIHDGQDVWVEGPEGGRIRVKAMVTRRVAPGVAFTPFHF
                     GGRYQGKDLRERYPQGCDPYVLGESCNVSMTYGYDSVTAMQETKCSLCRIKPA"
     gene            4684..5268
                     /gene="fdh3B"
                     /locus_tag="PK144_14400"
     CDS             4684..5268
                     /gene="fdh3B"
                     /locus_tag="PK144_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HNF66964.1"
                     /translation="MARMKFLCDAERCIECNACVTACKNEHEVPWGVNRRRVVTLNDG
                     EPGEKSISVACMHCSDAPCMAVCPVDCFYQTDNGIVLHDKDLCIGCGYCFYACPFGAP
                     QYPQKQAFGVRGKMDKCTFCAGGPEPDNSEAEFHKYGRNRIAEGKLPLCAEMCSTKAL
                     LAGDGDVVSDIYRARVLKRGGRPQTWGWDKAYPA"
     gene            5265..5486
                     /locus_tag="PK144_14405"
     CDS             5265..5486
                     /locus_tag="PK144_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66965.1"
                     /translation="MKRALVCLPLLAAALGGCYEPPGEVVLHRPHAYKGKDDALRTVL
                     TDPAFAERLRARQRAVQDVRIAGLKEAAL"
BASE COUNT          927 a         2017 c         1907 g          764 t
ORIGIN      
        1 gtcgagttcg aggtgatcgg cctcgcgcgc accgtcatcg gcgccggcgg cgagctgcag
       61 gccgtcgccg gtgcgcgtga cggcgagcgg cctgccggcg gcatcgacga gttcgatcag
      121 gccgctgccc gggggcagcg gcagcacggc cggggcctcg tcaccccaga acagccggcc
      181 gctgatcttg tgcgtgccgg ccggcaggcg cagcgccggg tggccgtcgt gcagcagcac
      241 cagcgccggt tcgccgtcgg ccccgaccgc gcgcgggcgc agcgcggcgt caccgggcag
      301 cagcagccag tcctcgcgat cgagccgcca cgtgccgctg aacagcgcgc cgtcggcggc
      361 gagttcgatg cgcagccgcc cggcccagac gcagcgccag tcgcccggcc cgccggcgag
      421 cgcgcagcgg cccgccggcg catcggccgg ccgcacccag ccgatccacg gccgcagcgg
      481 ctcgggcacg tcggtcgcat cgaaggcgga tgccggcagg gcaagggcca gcatcagccc
      541 cggcgacagc aggaaacgca gcaggaaaga cagcaggggc ggcatcgaca cgctttccac
      601 agacatccgc agacggacgg gatggcgcag agcgtagcgg ggcgcaccgg ccggtggcgc
      661 aggccggctg cgtcattctg acccgcgccg gatcacccgg gcgcgaccgg tcacacggcc
      721 agaatgcggc ggtgcccgga caagtgtctg tcatggaacg ataaagttga tggcacatgg
      781 tttgctttcc gcgagcaccg cccgaggaaa taccccatga cgaccatgga cgccgcgccg
      841 acgaccgcgc ccgacgaaga cgaccgcctg cgcgccggca cctgggcgct gctcgcagca
      901 ctgctgcgcc ggccgccgac ggcggagctg ctcgcgcaac tggcggcgat cgacacgccg
      961 ccggccggcg agggcggcgc gctggcggcg gcctggcagg acttgcgcgc ggcggccgcg
     1021 gtggccgatc cggtcgcgct cgagcgcgaa tatcacgcgc tgttcatcgg catcggccgc
     1081 ggcgagctgc tgccgttcgc gtcctggtat ctgaccggct tcctgatgga aaagccgctc
     1141 ggcgaactgc gggccgatct cgcccggctc ggcttcgagc gccagcccgg cgtctgcgag
     1201 cccgaggacc acatcgcagc cctcgccgag gtgctcggcc tgctgatcgg cgagggctcg
     1261 gcggcggccg cgggcttctt cgcgcatcac gtcgagccct gggccgggcg actgtttgcc
     1321 gacctgcaga cggcggaatc ggcgcgcttc taccgcgctg ccggccgctt cgccgaagcc
     1381 ttccttgcct tcgaacgcca gtacggcgcg atgccggcgc tcgcgccgtc gtcggtgccg
     1441 gccggctgaa gtcagccggc accgccagca atcaccggaa ctcccggaac ccgcatcacg
     1501 acgcgcccgg acgcacagga gcaccgacat gagcgagaaa acccctgccg ccggcctcga
     1561 ccgccgcgct ttcctgaaac tggtcggcag cggtgccggc gccggggccg tggcgctggc
     1621 gctgccgggc gtggcggtcg ccgcaccggc cgccgtgcgg cccgaacccg aagaagccgg
     1681 gcggctcgat gccggctatc gcgagaccgc gcacgtacgc gcctactacg acacctgccg
     1741 ctgaggccgt ccgcatgaaa ctcgtcagga aacaggccgg ggcacggccg gcagccgctg
     1801 cgacctcgcc ggtcgcggcc ttcctcggcg cgaccgtcga ccggcgcgcc ttcctcaaat
     1861 cctcgggact cgcgctcggt gcgaccgcgc tcggggccgc gctcgggccg acgatgatgc
     1921 gtcgcgcctc ggccgcgacc gacgccggca cccacgcgag caacgcggcg gtcgagatca
     1981 agcgcacgat ctgctcgcac tgctcggtcg gctgcggcat ctacgccgag gtgcagaacg
     2041 gcgtctgggt cggccaggag ccggccttcg accatccgtt caacatgggc gcgcactgcg
     2101 cgaagggcgc ctcgatccgc gagcacggcc acggcgagcg gcgcctgaag tacccgatga
     2161 agctcgtcga cggccagtgg cagcgcatct cgtgggacac ggcgctgagc gaggtgtcgg
     2221 cgcggctgct gaagatccgc gaggaatccg gccccgacgc cgtctactgg ctcggctcgg
     2281 ccaagcacag caacgagcag gcctatctgt tccgcaagtt cgcgtcgatg tggggcacga
     2341 acaacgtcga tcaccaggcg cgcatctgcc actcgaccac ggtcgccggc gttgccaaca
     2401 cctggggcta cggcgcgatg acgaactcct acaacgacat cgcctacgca aaatccatcc
     2461 tgatgatcgg cagcaacgcc gccgaggcgc acccggtgtc gatgcagcac atcctgcgcg
     2521 ccaaggagga cggcgcgaag ctgatcgtca tcgacccgcg cttcacgcgc accgccgcgc
     2581 acgccgatca ctacgtgcgc atccgccccg gcaccgacgt gccgttcatc tggggcctgc
     2641 tctggcacgt gttccagaac aactggcagg acgacagctt cctgcagcag cgcgtgttcg
     2701 gcatggacga ggtgcgcaag gaagtcgaga agtggccgcc ggccgaggtc gagcgcgtca
     2761 ccggcgtcca ggagaaggat gtccttgcgg tcgcgaagct gatggccgag caccgccccg
     2821 gcaccttcgt ctggtgcatg ggcggcacgc agcacacgat cggcaacaac aacacgcgcg
     2881 cctactgcat cttccagctc gcgctcggca acatgggcaa ggccggcggc ggcaccaaca
     2941 tcttccgcgg tcatgacaac gtccagggcg cgaccgactt cggcgtgctc gccgacagcc
     3001 tgcccggcta ttacggcctc gccgaaggtt cgtggaagca ctgggcgaag gtctggggcg
     3061 tcgactacga gtggctgaag gcgcgcttcg acccgaacgc ctacgacggc ggctccggca
     3121 agccggtcaa gccgatggaa acctccggca tcccggtgtc gcgctggatc gacggcgtgc
     3181 tcgaggacaa ggcgctgatg acgcagcgcg acaacgtgcg cggcatggtg ttctggggcc
     3241 acgcgccgaa ctcgcagacg cgcggcaccg agatgaagaa ggcgttcgag aagctcgaca
     3301 cgctggtcat cgtcgatccg tacccgaccg tctcggcagt catgcacgag cgccgggacg
     3361 gtgtgtatct gctgccggcc gcgacgcagt tcgagaccta cggctcggtg acggcctcga
     3421 accgctcggt gcagtggcgc gagcaggtca tcgcgccgct gttcgaatcg cgccccgatc
     3481 acacgatcct ctaccagctc gcgaagaagt tcggctatgc cgacgagctc tgcaaaaacg
     3541 tcaaggtcac gaacgacgag ccgtcgatcg aggatctggt gcgcgaatac aaccgcggca
     3601 tgtggacgat cggctacacc ggccagtcgc ccgaacgcct gaaggcgcac cagaagaact
     3661 ggcacacctt caacaccaga accctgcgcg ccgagggcgg cccgctcgat cgcgagttct
     3721 acggcctgcc gtggccgtgc tggggcacgc cggaaatgaa gcaccccggc actgccaacc
     3781 tctacgacac cagcctgccg gtcgccgacg gcggcctgtg cttccgcgcc aacttcggcg
     3841 tcgagcgcaa cggcgtgaac ctgctcgccg agcattccta tccggccggt tccgagatcg
     3901 aggatggtta tcccgagttc gacgacaagc tggtcaagaa gctcggctgg tgggacgacc
     3961 tcaccgacgc cgaaaagacc gccgccgagg gcaagaactg gaagacggac ctctccggcg
     4021 gcatccagcg cgtcgtcatc aagcacggct gtgcaccgtt cggcaacgcc aaggcgcgct
     4081 gcgtggtgtg gaatttcccc gatccggtgc cgatccaccg cgaaccgctg tatacctcgc
     4141 gccgcgatct ggtcgggcag tacccgacct atgccgatcg caaggcctac tggcgcctgc
     4201 cgacgcgcta cgcgtcgatc cagcagcagg acttcagcca ggacttcccg atcatcctga
     4261 cctcgggccg gctggtcgaa tacgagggcg gcggcgacga gacgcgctcg aatccgtggc
     4321 tcgccgagct gcagcagcac atgttcgtcg agctgaaccc gaaggacgcc aacgacgccg
     4381 gcatccatga cggtcaggac gtctgggtcg aaggccccga gggcgggcgc atccgcgtga
     4441 aagccatggt cacgcgccgc gtcgcgcccg gcgtggcgtt cacgcccttc cacttcggcg
     4501 gccgttacca gggcaaggac ctgcgcgagc gttatccgca gggctgcgac ccctacgtgc
     4561 tcggcgaatc ctgcaacgtg tcgatgacct acggctacga ctcggtcacc gcgatgcagg
     4621 aaaccaagtg ctcgctctgc cgcatcaagc cggcctgacc ccgaactcac ggagaccgcc
     4681 gccatggccc ggatgaaatt cctctgcgac gccgagcgct gcatcgagtg caacgcctgc
     4741 gtcaccgcct gcaagaacga gcacgaagtg ccctggggcg tgaaccgccg ccgcgtcgtg
     4801 accctcaacg acggcgagcc cggcgagaag tcgatctcgg tcgcgtgcat gcactgctcg
     4861 gacgcgccgt gcatggccgt ctgcccggtc gactgcttct accagaccga caacggcatc
     4921 gtgctgcacg acaaggacct ctgcatcggc tgcggctact gcttctacgc ctgcccgttc
     4981 ggcgcgccgc agtacccgca gaagcaggcc ttcggcgtgc gcggcaagat ggacaaatgc
     5041 accttctgcg ccggcggccc ggagcccgac aactccgagg ccgagttcca caagtacggc
     5101 cgcaaccgca tcgccgaagg caagctgccg ctgtgcgccg agatgtgctc gaccaaggcg
     5161 ctgctcgccg gcgacggcga cgtggtctcg gacatctacc gcgcgcgcgt gctgaagcgc
     5221 ggcggccgtc cgcagacctg gggctgggac aaggcgtacc cggcatgaaa cgcgcgctcg
     5281 tctgtctgcc gctgctcgcc gccgcgctcg gcggctgtta cgagcctccg ggcgaggtcg
     5341 tgctgcaccg gccgcacgcc tacaagggca aggacgatgc gctgcgcacc gtgctcaccg
     5401 atccggcctt cgccgagcgt ctgcgcgcgc gccagcgcgc cgtgcaggac gtgcgcatcg
     5461 ccgggctgaa ggaggccgcg ttatgagtcc gaaccgctcc ggaacctctg cccgccgcgc
     5521 actggcgctg cggcgcatcg cggtcgcgct gctgctgttt gtcgccatcg ccgcggcgct
     5581 gccgtttgcc gggcgctggc tggtggcacc ggccg
//
LOCUS       DAOQIE010000260         5577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8095_length_5577_cov_6.961427, whole genome shotgun sequence.
ACCESSION   DAOQIE010000260 DAOQIE010000000
VERSION     DAOQIE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8095_length_5577_cov_6.961427"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PK144_14410"
     CDS             complement(<1..716)
                     /locus_tag="PK144_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF66966.1"
                     /translation="MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNV
                     ETKPGIGYPKEWENQKKWNGGWTRRKDGKIEPKIGGKWRVLANLFANPDLPAIDDYYE
                     PWDYDYAHLQNAPESKAMPVARPRSVISGERMQKIEWGPNWEEILGTEFAKRSKDYNF
                     ENVQKEIYGEFENTFMMYLPRLCEHCLNPACVAACPSGAIYKREEDGIVLIDQDKCRG
                     WRMCVSACPYKKIYYNWNTG"
     gene            complement(831..4586)
                     /locus_tag="PK144_14415"
     CDS             complement(831..4586)
                     /locus_tag="PK144_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNF66967.1"
                     /translation="MSHFLDRLTFFRKTREPFADGHGARTDEDRGWEDAYRLRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRDDLPNHEPRGCQRGASYSWY
                     LYSANRVKYPMVRGRLMQLWREARATKDPVEAWASIASDPAKAAEYKTRRGLGGFVRA
                     DWAEVNELIAAANAWTIKQYGPDRVSGFSPIPAMSMVSYAAGSRYLSLIGGNCLSFYD
                     WYCDLPPASPMIWGEQTDVPESADWYNSSFIMMWGSNVPQTRTPDAHFMTEVRYKGTK
                     TVVVTPDYSEASKFADIWLHPKQGTDAALAMAMGHVILREFHLDAKSEYFDKYVRETT
                     DFPVLVVLDARADGSYVNGRFLRAADLEGGAGQANNPEWKTLAFDEKTGNLIVPNGSI
                     GFRWGEKDGLAGKWNLEPREALSGKDAQLRLSVNEIRDEVVPVAFPYFGATKHPHFAH
                     TEHDEIQLRNVPAKRVTLADGSTALVATVYDLTLAQYGLDRGLGGANVAASFDDDVPY
                     TPAWQEKITGVPRAQVIAVARQFAENADRTHGRSMIIIGAAMNHWYHMDMNYRGVINL
                     LMMCGCIGQSGGGWAHYVGQEKLRPQTGWTALAFALDWNRPPRHMNGTSFFYIHSSQW
                     RHEKLAMSEILSPLADPKDWQGTQIDYNVRSARMGWLPTAPHFNVNTLKFAKEAVAAG
                     KAPVEHLVAGLKSGKVDFAFADPDNPVNFPRNLFVWRSNLLGSSGKGHEYFLKYLLGT
                     THGVQGKDLGEEGGEKPLEVQWREGATGKLDLLVTLDFRMSTTCMYSDIVLPTATWYE
                     KNDLNTSDMHPFIHPLSAAVDPAWESRTDWDIFKGIAEKFSEVCRGHLGVEQDIVALP
                     TQHDTPNELAMPEVRDWKKGECELIPGKTGPSLVVVERDYPNTFKKFTSVGPLLDKLG
                     NGGKGISWNTVHEVEALATLNYTVLEDGVSKGRPRIDSDIDAAEVILMLAPETNGHVA
                     VKSWAALSKITGRDHTHLALPKEHEKIRYRDIQAQPRKIISSPTWSGLEDENVSYNAG
                     YTNVHELIPWRTVTGRQQFYQDHPWMLGFGEAMCAYRPPVDMKAAEPLLGKRQNGQPE
                     IVLNFITPHQKWGIHSTYTDNLLMLTLSRGGPIIWISEVDAKKAGIEDNDWIEAFNLN
                     GAIAARAVVSQRVPEGMSMMYHAQEKIVNTPGSEVTGTRGGIHNSVTRTVLKPTHMIG
                     SYAQQSYGFNYYGTVGANRDEFIVVRKMAKIDWMDEEVDSDAQAAGH"
     gene            5076..>5577
                     /locus_tag="PK144_14420"
     CDS             5076..>5577
                     /locus_tag="PK144_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNF66968.1"
                     /translation="MNVAYGELDPGLRALVEGTASVTGDAFFAALVAGVAAALGVRWC
                     FVAEFASVRTRVRMLAYRGPEGALAPVEFDLAGTPCEAVLGGECRHYERGVSERYPLD
                     AGLVRRGAEGYLAIPLRRGSGEVLGHLAVFDDRPLSVRPAQLALFQVFGARAAAELER
                     VRTERAL"
BASE COUNT          766 a         1819 c         1945 g         1047 t
ORIGIN      
        1 ttgccggtgt tccagttgta atagatcttc ttgtacgggc aggccgacac gcacatgcgc
       61 cagccgcggc acttgtcctg atcgatcagg acgatgccgt cctcctcgcg cttgtagatc
      121 gcacccgacg ggcacgccgc gacgcaggcc ggattcaggc agtgctcgca caggcgcggc
      181 aggtacatca tgaaggtgtt ttcgaattcg ccgtagattt ccttctggac gttttcgaag
      241 ttgtagtcct tcgagcgctt cgcgaattcg gtgccgagga tttcctccca gttcggaccc
      301 cattcgatct tctgcatgcg ctcgccggag atcaccgagc gcgggcgcgc gaccggcatc
      361 gccttcgact cgggcgcgtt ctgcaggtgc gcgtagtcgt agtcccaggg ctcgtagtag
      421 tcgtcgatgg ccggcaggtc gggattcgcg aacaggttcg cgagcacgcg ccacttgccg
      481 ccgatcttcg gctcgatctt gccgtccttg cggcgcgtcc agccgccgtt ccatttcttc
      541 tggttctccc attccttcgg gtagccgatg ccgggcttgg tctcgacgtt gttgaaccac
      601 gcgtattcca tgccttcgcg cgaggtccac acgttcttgc aggtcaccga gcaggtgtgg
      661 cagccgatgc acttgtccag attcaggacc atgccgatct gtgcacgaac tttcattttc
      721 tctcttcctc gtcgggaatt cggtcgcgac cgcggtcgtt tcgcgcgcag tcgcgcggcg
      781 gcgcacgccg cggccccggg ggggccgcgg gttgcctgaa cctcggtgtc tcagtgaccc
      841 gccgcctgcg cgtcggagtc gacttcctca tccatccagt cgatcttcgc catcttgcgc
      901 acgacgatga attcgtcgcg gttggcgccg accgtgccgt agtagttgaa gccgtagctc
      961 tgctgcgcgt agctgccgat catgtgcgtg ggcttgagca ccgtgcgcgt cacggagttg
     1021 tgaatgccgc cgcgggtgcc ggtgacttcc gagccgggcg tgttcacgat cttctcctgc
     1081 gcgtggtaca tcatcgacat gccttccggc acgcgctgcg agaccacggc ccgggcggcg
     1141 atcgcgccgt tgaggttgaa ggcctcgatc cagtcgttgt cctcgatgcc ggccttcttc
     1201 gcgtcgacct cgctgatcca gatgatcgga ccgccgcgcg acagcgtcag catcagcagg
     1261 ttgtccgtgt acgtgctgtg gatgccccac ttctggtgcg gagtgatgaa gttcagcacg
     1321 atttccggct ggccgttctg gcgcttgccg agcagcggtt cggccgcctt catgtccacc
     1381 ggcggacggt acgcgcacat ggcctcgccg aagccgagca tccacgggtg atcctgatag
     1441 aactgctggc ggccggtgac ggtgcgccac ggaatcagtt cgtggacgtt ggtgtagccg
     1501 gcgttgtacg agacgttctc gtcttccagc cccgaccagg tcggcgagga gatgatcttg
     1561 cgcggctgcg cttggatgtc gcggtagcgg atcttctcgt gttccttcgg cagcgcgagg
     1621 tgcgtgtggt cgcggccggt gatcttcgac agcgccgccc aggacttcac ggcgacgtgg
     1681 ccgttggtct cgggggcgag catcaggatc acctcggcgg cgtcgatgtc gctgtcgatg
     1741 cgcggccggc ccttggagac gccgtcctcc agcaccgtgt agttcagcgt cgcgagcgcc
     1801 tcgacctcat gcacggtgtt ccacgagatg cccttgccgc cgttgccgag cttgtccagc
     1861 agcggaccga ccgacgtgaa cttcttgaac gtgttcggat agtcgcgctc gacgacgacg
     1921 aggctcggtc cggtcttgcc cgggatcagc tcgcactcgc ccttcttcca gtcgcgcacc
     1981 tccggcatcg ccagttcgtt cggcgtgtcg tgctgggtcg gcagcgcgac gatgtcctgc
     2041 tcgacgccga ggtggccgcg gcagacctcg gagaacttct cggcgatgcc cttgaagatg
     2101 tcccagtcgg tgcgcgactc ccacgccggg tccacggccg ccgacagcgg gtggatgaac
     2161 gggtgcatgt cggaggtgtt gagatcgttc ttctcgtacc aggtcgccgt cggcagcacg
     2221 atgtccgagt acatgcaggt cgtggacatc cggaagtcca gcgtcaccag caggtcgagc
     2281 ttgccggtcg cgccctcgcg ccactgcact tccagcggct tctcgccgcc ttcctcgccg
     2341 agatccttgc cctgcacgcc gtgcgtggtg ccgagcaggt acttcaggaa gtactcgtgg
     2401 cccttgccgc tggagccgag caggttcgag cgccacacga acaggttgcg cgggaagttg
     2461 accgggttgt ccggatcggc gaacgcgaag tcgaccttgc cggacttcag gcccgcgacc
     2521 aggtgctcga ccggcgcctt gccggcggcg acggcttcct tcgcgaactt cagcgtgttg
     2581 acgttgaagt gcggcgcggt cggcagccag cccatgcgcg cggagcgcac gttgtagtcg
     2641 atctgcgtgc cctgccagtc cttcgggtcg gcgagcggcg agaggatctc gctcatcgcg
     2701 agcttctcgt gccgccactg actggagtgg atgtagaaga acgaggtgcc gttcatgtgc
     2761 cgcggcgggc ggttccagtc gagcgcgaac gcgagcgcgg tccagccggt ctgcgggcgg
     2821 agcttctcct gaccgacgta gtgcgcccag ccgccgccgc tctgaccgat gcagccgcac
     2881 atcatcaaca ggttgatgac gccgcggtaa ttcatgtcca tgtggtacca gtgattcatc
     2941 gcggcgccga tgatgatcat ggaccggccg tgcgtcctgt cggcgttctc ggcgaactgg
     3001 cgggcgacgg cgatgacctg cgcgcgcggc acgccggtga tcttctcctg ccaggccggc
     3061 gtgtacggca cgtcgtcatc gaagctcgcc gcgacgttgg caccgcccag accgcgatcg
     3121 aggccgtact gcgcgagcgt caggtcgtag acggtcgcga ccagcgccgt gctgccgtcg
     3181 gcgagcgtga cgcgcttcgc cggcacgtta cgcagctgaa tctcgtcgtg ctcggtatgc
     3241 gcgaagtgcg ggtgcttcgt ggcgccgaag tacgggaagg cgaccggcac gacctcgtcg
     3301 cggatctcgt tgaccgacag ccgcagttgc gcgtccttgc cggacagcgc ctcgcgcggc
     3361 tccaggttcc acttgccggc gaggccgtcc ttctcgcccc agcggaagcc gatcgagccg
     3421 ttcgggacga tgagattccc ggtcttctcg tcgaacgcga gcgtcttcca ctcggggttg
     3481 ttcgcctggc cggcgccgcc ttcgaggtcg gcggcccgca ggaagcggcc gttgacgtag
     3541 ctgccgtcgg cgcgcgcatc gagcacgacg aggacgggga agtcggtggt ctcgcggacg
     3601 tacttgtcga agtactcgct cttggcgtcg aggtggaact cgcgcaggat cacgtgaccc
     3661 atcgccatcg cgagcgcggc gtcggtgccc tgcttcgggt gcagccagat gtcggcgaac
     3721 ttcgaggctt ccgagtagtc gggcgtgacc acgacggtct tcgtgccctt gtagcggacc
     3781 tcggtcatga agtgcgcgtc gggcgtgcgc gtctgcggca cgttcgagcc ccacatcatg
     3841 atgaagctgg agttgtacca gtcggccgat tccggcacgt cggtctgctc gccccagatc
     3901 atcggcgagg ccggcggcag gtcgcagtac cagtcgtaga acgacaggca gttgccgccg
     3961 atcagcgaca ggtaccggct gccggcggcg taggacacca tcgacatcgc cgggatcggc
     4021 gagaagcccg agacgcggtc cgggccgtac tgcttgatcg tccaggcgtt ggccgcggcg
     4081 atcagttcgt tgacctccgc ccagtcggcg cgcacgaagc cgccgaggcc gcgcctggtc
     4141 ttgtactcgg ccgccttcgc cggatcagag gcgatgctcg cccaggcctc gaccgggtcc
     4201 ttcgtcgcgc gcgcctcgcg ccagagctgc atcaggcgcc cgcgcaccat cgggtacttc
     4261 acgcggttag cgctgtacag ataccagctg taggacgccc cgcgctggca gccgcgcggc
     4321 tcgtggttcg gcaggtcgtc gcgggtgcgc gggtagtcgg tctgctgggt ttcccaggtg
     4381 accagaccgt tcttgacgta gatcttccac gagcacgagc cggtgcagtt cacgccgtgc
     4441 gtcgagcgca cgatcttgtc gtgctgccag cgcaggcggt aggcgtcctc ccagccgcgg
     4501 tcctcgtcgg tgcgggcacc gtggccgtcc gcaaacggtt cccgggtctt gcggaagaag
     4561 gtcagtcggt cgagaaagtg actcatcgtg cgttcctctg tcctcggtgc gcggtgccgg
     4621 cccgatggcg gacgggcttg tcggatacgg attatatacg gagccgcgag cttgcggcgc
     4681 cggggctgcg tgcaagctaa atcacggacg cacaaggaaa ttgaccggca tcaagtgcga
     4741 ccggacgggg gaggcgggcg ggccgtccgc ctacctcctg cgggatagcg gggcgcgcgg
     4801 cgcaagtgcg cgtgccggcg cacatttcgc agcgtgcagc ccgggggatg ccgccctgcg
     4861 ggggcgatgg gggtaggggg gcggctaccc cccgtaccgg cggctgcccg gcgccggaag
     4921 cagcggatca cgaagcgcgc agggcacagc cgggctgcgg cgggctcggg tcaccggtgc
     4981 cgcgtgcgct gcggctgtgc cggacacggc tacagtgagg ggcgtgcaag gtccggcgca
     5041 ggggcgtgcg gaccgacctc gcgggggcag catccatgaa cgtcgcatac ggcgaactcg
     5101 atccgggcct gcgcgcactg gtcgagggca cggcctcggt caccggcgat gccttcttcg
     5161 cggcgctggt cgccggggtg gccgccgcgc tcggcgtgcg ctggtgcttc gtcgccgagt
     5221 tcgccagcgt gcgcacgcgc gtgcgcatgc tcgcctaccg cgggccggag ggcgcgctgg
     5281 caccggtcga gttcgatctg gccggcacgc cgtgcgaggc ggtgctcggc ggcgagtgcc
     5341 ggcactacga gcgcggcgtt tcggagcgtt atccgctcga tgccggcctc gtgcgccgcg
     5401 gcgccgaagg ctatctggcg atcccgctgc ggcgcggcag cggcgaggtg ctcgggcatc
     5461 tggcggtgtt cgacgaccgg ccgctgtcgg tgcgcccggc gcagctcgcg ctgttccagg
     5521 ttttcggcgc ccgcgccgcg gcggagctgg agcgcgtgcg caccgagcgg gcgctgc
//
LOCUS       DAOQIE010000261         5573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8109_length_5573_cov_6.638818, whole genome shotgun sequence.
ACCESSION   DAOQIE010000261 DAOQIE010000000
VERSION     DAOQIE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8109_length_5573_cov_6.638818"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK144_14425"
     CDS             complement(<1..192)
                     /locus_tag="PK144_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNF66969.1"
                     /translation="MNRQDTAATRALIAEEVAWARGLMLQGAPLATRLPGRVGWELRL
                     VVQGGLRILDKIEAIDHAVL"
     gene            complement(192..794)
                     /locus_tag="PK144_14430"
     CDS             complement(192..794)
                     /locus_tag="PK144_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF66970.1"
                     /translation="MNQQDKSFLTTFTGVMAILVVLAAVIFLVAQLIGKLNRPADDGS
                     RMQAQAEDRIKPVARVAVADPNKKPVKLSAKDIVTQVCSSCHGAGVLGAPKIGNAEDW
                     TPRVAQGLDTLVSHAVNGFKGMPAKGGSAALSDADIRSAVIYMLKDSGVKLDAAPAKA
                     DAAAAAAPAAPAAPAAPAAPAAACTCRWTTAPAMGWVWTS"
     gene            1112..1188
                     /locus_tag="PK144_14435"
     tRNA            1112..1188
                     /locus_tag="PK144_14435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1146..1148,aa:Arg,seq:ccg)
     gene            1262..2188
                     /locus_tag="PK144_14440"
     CDS             1262..2188
                     /locus_tag="PK144_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNF66971.1"
                     /translation="MVILQTPGGFDLLFRIRGSIVRDILRPLLATTALATLVTVLHES
                     VLPFRYSMTVTPFSLIGLTLAIFLGFRNSVSYDRYWEGRKLWGELLNACRSLARLALT
                     TIDAPAGAADARPTAFVHRLMAFAHALRHHLRGSDPLPDLERLLGPQAAARPAAAHNV
                     PLALLQELGEHLHGARRDLRIDPLLAAHAESRLDRLADVLGGCERIRNTPLPYPYRLL
                     LHRTVYFYCLLLPFGLIDTVGRMTPVVVLFLAYTFFGLDALGEQIEEPFGEMPHDLPL
                     LAISTTIEADLLELLGERERPAAVRPVDGVLL"
     gene            complement(2204..3709)
                     /gene="glpK"
                     /locus_tag="PK144_14445"
     CDS             complement(2204..3709)
                     /gene="glpK"
                     /locus_tag="PK144_14445"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNF66972.1"
                     /translation="MDERYVLAIDQGTTSTRALLFDHTGRVAGCAQRELTQHFPQPGW
                     VEHDASEILASVLTTIAEVLTVTRTPVQAIAALGITNQRETVVIWEKATGRPIHRAIV
                     WQSRQSEGICAQLRAQGAEPLIRERTGLLIDPYFSGTKLRWLLDQLPGARARAGRGEL
                     LAGTIDSWLVWNLSGGRRHVTDASNASRTLLYDIHARRWDDELLALLDVPRALLPEVC
                     ASSGVLGTTDPRLTLGVALPIAGLAGDQQAALFGQGCHAPGMAKNTYGTGCFLLMHTG
                     AQAVCSRHGLLTTIAWQIGDQLEYALEGSIFVAGSAVQWLRDGLKLIDTAADSEALAQ
                     SVASTGGVYCVPAFVGLGAPYWRSDVRGALFGLTRGSGRAEICRAVLEAIAFQTRDVL
                     DAMQADAGLALERLRVDGGAVANAFLMQFQSDILGVPVERPAQPEATAFGAAALAGLA
                     TGFWADRAAIAACCRPDRRFEPALPAAGREASYAGWRQAVQATLGFRIDPA"
     gene            complement(3737..5260)
                     /gene="glpD"
                     /locus_tag="PK144_14450"
     CDS             complement(3737..5260)
                     /gene="glpD"
                     /locus_tag="PK144_14450"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HNF66973.1"
                     /translation="MAVDADCDLLVIGGGINGAAIARDAAGRGLRVLLCERDDLAAHT
                     SGASTRLLHGGLRYLEHGELRLVAQALAERERLLRLAPQLSQPLDFVLLPARGLRPGW
                     LIRLGLFLYDHLDFGRRTLPATRRIETGALALGAALRPDCAGSAWMYADGWTDDARLT
                     VLSALDAAERGAAIRTRTTVTAAQREGRRWRVQLQARDGGQQVTARALVNAAGPWVEA
                     VLGTLGAGGCAQAARPRLRLVRGSHIVVPRLYDGPQACVLQQPDRRIVFTIPYEQDWT
                     LIGTTEVDCARPGDAHTDAAEIAYLCTALAPYFRSPPTPRDVVWHYAGVRPLLDDGRA
                     SSRVSRDWRLESDAGPPPLVSVYGGKLTTCRRLAEAVVDRLAAGLGCRAPAWTAGGPA
                     LPGGDFDDREALLRSLRATRPWLPPALAARLVRSYGTRAARLLGAANALAGLGTDFGA
                     GLHAAEVDYLVQTEWARTAEDVLWRRSKLGLRIEAGAAARLADYLAEAVPRLSGGHA"
BASE COUNT          749 a         2052 c         1990 g          782 t
ORIGIN      
        1 cagcaccgca tggtcgatcg cctcgatctt gtccagtatc cgcaacccgc cctgtaccac
       61 caggcgcagt tcccaaccga cccggcccgg cagccgcgtg gccagcggcg caccctgcaa
      121 catcagtcca cgagcccagg cgacctcttc ggcgatcagc gcgcgggtgg ctgcggtgtc
      181 ctgccggttc atcagctggt ccacacccag cccatggcgg gcgcagtcgt ccagcggcag
      241 gtacaggcgg cagcaggtgc ggccggggca gcaggtgcgg ccggagcggc aggggcagcc
      301 gcagcggcgg catcggcctt ggccggggcg gcgtcgagct tcacgccgga atccttgagc
      361 atgtagatga cggccgagcg gatgtcggca tcgctcagcg cggcgctgcc gcccttggcc
      421 ggcatgccct tgaagccgtt gacggcgtgg ctgaccagcg tgtcgagacc ctgcgccacg
      481 cgcggggtcc agtcctcggc gttgccgatc ttcggcgcac cgagcacgcc ggcgccgtgg
      541 caggacgagc agacctgggt gacgatgtcc ttcgccgaca gcttcaccgg cttcttgttc
      601 ggatcggcga cggcgacgcg cgcgacgggc ttgatgcggt cctcggcctg cgcctgcatg
      661 cgcgagccgt cgtcggccgg gcggttcagc ttcccgatca gctgcgccac caggaagatc
      721 acggcggcca gaacgaccag gatcgccatc acgcccgtga acgtcgtcag gaaggatttg
      781 tcttgctggt tcacgcgttc acctctccgg attgtgctgg atgtcatccg gtccgcgccg
      841 gtccggcctg gctgcatggg gcaatgcggg cgtcacggcg gcagcaccgg gacggacgca
      901 gggccccgca ggccgcgcga gccgtgaagt cttttgtgag cagggttggg tatcggcagg
      961 tactgcagga aattgcggtg gcagccgcaa ggcgcggcat tgtagcggca tcggcaagac
     1021 cgcgggaagc acagggaaat cgcctccgcc ccggcggcgg gccatggacg gcgccgcgcc
     1081 gcagcggtat cctgcgcgcc cttcctgcaa tgcgcccgta gctcagctgg atagagcgct
     1141 gccctccgga ggcagaggtc aggggttcga atcccttcgg gcgcgccatt cctcaccccg
     1201 ccgttcccgc tgtctgcgct ccccgacgtc accgattcac ccgcggctcc ggtcacgcgc
     1261 catggtcatc ctgcagacgc ccggcggttt cgatctgctg ttccgcatcc gcggctcgat
     1321 cgtgcgggac atcctgcgtc cgctgctggc gacgacggcg ctcgcgacgc tggtcacggt
     1381 gctgcacgag tcggtgctgc cgtttcgtta ttcgatgacc gtcacgccgt tcagcctgat
     1441 cggcctgacc ctcgcgatct ttctcggctt ccgcaacagc gtcagctacg accgctactg
     1501 ggaaggccgc aagctctggg gcgaactgct gaacgcctgc cgcagcctgg cccgactggc
     1561 gctgacgacg atcgatgccc cggccggcgc cgccgatgcg cggccgacgg cgttcgtgca
     1621 ccggctgatg gcgtttgcgc atgcgctgcg ccaccacctg cgcggcagtg atccgctccc
     1681 cgacctcgaa cgcctcctcg gcccgcaggc cgccgcccgt ccggcggcgg cgcacaacgt
     1741 gccgctggcg ctgctgcagg aactcggcga gcacctgcac ggcgcgcgcc gtgacctgcg
     1801 catcgacccg ctgctcgccg cgcatgccga atcccggctc gaccggctcg ccgacgtgct
     1861 cggcggctgc gaacgcatcc gcaacacgcc gctgccgtac ccgtaccggc tgctgctgca
     1921 ccgtaccgtg tacttttact gcctgctgct gcccttcggc ctgatcgaca cggtcggccg
     1981 gatgacgccg gtggtcgtgc tgttcctcgc ctacacgttc ttcggcctcg atgccctcgg
     2041 cgagcagatc gaggaaccgt tcggcgagat gccgcacgat ctgccgctct tggcgatcag
     2101 cacgacgatc gaggccgacc tgctcgaact gctcggcgag cgcgaacggc cggcggccgt
     2161 gcggccggtc gatggcgtgc tgctgtgaga ccgggccgcg ccctcaggcc ggatcgatcc
     2221 tgaaccccag cgtcgcctgc accgcctgtc gccagcccgc gtagcttgcc tcgcgcccgg
     2281 cagccggcag cgccggctcg aagcgacgat cgggccggca gcaggcggcg atggcggcgc
     2341 gatcggccca gaagccggtc gccagcccgg cgagcgccgc ggcgccgaag gccgtggctt
     2401 cgggctgggc cgggcgttcg accggcacgc cgaggatgtc gctctggaac tgcatcagga
     2461 aggcgttggc gaccgcgccg ccgtcgacgc gcagccgttc gagcgcaagc cccgcatcgg
     2521 cctgcatcgc atcgagcacg tcgcgggtct ggaaggcgat cgcttcgagc acggcacggc
     2581 agatctcggc ccggccgctg ccgcgcgtca gcccgaacag cgcaccgcgc acgtcgcttc
     2641 gccagtacgg cgcgccgagc ccgacgaagg ccggcacgca gtagacgccg ccggtggagg
     2701 cgaccgactg cgccagcgcc tctgaatcgg cggcggtatc gatcagcttc aggccgtcgc
     2761 gcagccactg cacggccgag ccggcgacga agatgctgcc ttcgagcgcg tattcgagct
     2821 gatcgccgat ctgccacgcg atcgtcgtca gcagcccgtg gcgtgaacac accgcctgcg
     2881 cgccggtgtg catcagcagg aagcagccgg tgccgtaggt gttcttggcc atgcccggcg
     2941 cgtggcagcc ctgcccgaac agcgccgcct gctggtcgcc ggccaggccc gcgatcggca
     3001 gcgcgacgcc gagcgtcagc cgcggatcgg tcgtgccgag cacgccgctc gatgcgcaga
     3061 cctcgggcag cagggcgcgc ggcacgtcga gcagcgcaag cagttcgtcg tcccagcgcc
     3121 gggcgtggat gtcgtagagc agcgtgcgcg aggcgttgct cgcgtcggtg acgtgccgcc
     3181 ggccgccgct gaggttccag accagccagc tgtcgatcgt gccggccagc agctcgccgc
     3241 gtccggcccg tgcgcgggcg ccgggcagct gatcgagcag ccacctgagc ttggtgccgg
     3301 agaagtacgg atcgatcagc aggccggtgc gctcgcggat cagcggctcg gccccttgcg
     3361 cgcgcagctg cgcgcagatg ccttcgctct ggcgcgactg ccagacgatg gcgcgatgga
     3421 tcggccggcc ggtggccttc tcccagatca cgacggtctc gcgctggttg gtgatgccga
     3481 gcgcggcgat cgcctgcacc ggggtgcgcg tgacggtcag cacttcggcg atcgtcgtca
     3541 gcacgctggc gaggatctcg gaagcgtcgt gctcgaccca gcccggctgc gggaagtgct
     3601 gcgtgagctc gcgctgcgcg cagccggcga cgcgcccggt gtggtcgaac agcagcgcgc
     3661 gcgtgctggt cgtgccctga tcgattgcga gcacgtagcg ttcgtccatg tctcctcccc
     3721 ggccgcggcc ggcggatcag gcgtgtccgc cgctcagccg cggtactgct tcggcaaggt
     3781 agtccgcgag ccgcgccgct gcccccgcct cgatgcgcag cccgagcttg ctgcgccgcc
     3841 acagcacgtc ctcggccgtg cgcgcccatt cggtctgcac caggtaatcg acctcggccg
     3901 catgcagccc ggccccgaag tccgtgccga gtccggcgag cgcattggcg gcaccgagca
     3961 gccgcgctgc gcgcgtgccg tagctgcgca ccagccgcgc cgccagcgcc ggcggcagcc
     4021 acggccgcgt cgcgcgcagg ctccgcagca gtgcctcgcg atcgtcgaaa tccccgcccg
     4081 gcagcgccgg cccgccggcg gtccaggccg gcgcgcgaca gccgagcccc gctgccagcc
     4141 ggtcgacgac ggcctcggcg aggcgccggc aggtggtcag cttgccgccg tacacgctca
     4201 cgagcggcgg cgggccggca tcggattcga gccgccagtc gcggctgacg cgcgagctgg
     4261 cacggccgtc gtcgagcagc ggacgcacgc cggcgtagtg ccagaccacg tcgcgcggcg
     4321 tcggcggcga gcggaagtac ggcgcgagcg ccgtgcacag gtaggcgatc tcggccgcat
     4381 cggtgtgcgc atcgccgggc cgcgcgcagt cgacctcggt cgtgccgatc agcgtccagt
     4441 cctgctcgta cggaatcgtg aacacgatgc gccggtccgg ctgctgcagc acgcaggcct
     4501 gcggcccgtc gtacaggcgc ggcacgacga tatggctgcc gcgcaccagc cgcagccgcg
     4561 gacgggcagc ctgcgcgcag ccgcccgccc cgagggtgcc gagcacggcc tcgacccacg
     4621 gcccggcggc gttgaccagc gcgcgcgccg tgacttgctg gcccccgtcc ctggcctgca
     4681 gctgcacgcg ccagcggcgg ccctcgcgct gcgcggccgt caccgtcgtg cgcgtgcgga
     4741 tggcggcgcc gcgttcggcg gcatcgagcg cgctcagcac cgtcagccgc gcgtcgtcgg
     4801 tccagccatc ggcatacatc caggcgctgc ccgcgcagtc cgggcgcagt gcggcgccga
     4861 gcgccagcgc gccggtttcg atgcggcgcg tcgccggcag cgtgcgccgg ccgaagtcga
     4921 ggtggtcgta caggaacagc ccgagccgga tcagccagcc gggacgcagg ccgcgcgccg
     4981 gcagcagcac gaagtcgagc ggctgcgaaa gctgcggggc gaggcgcagc aggcgttcgc
     5041 gctcggcgag cgcctgcgcg accagccgca gctcgccgtg ttcgaggtag cgcagccccc
     5101 cgtgcagcag cctggtgctg gcgccggagg tgtgcgcggc gaggtcgtcg cgctcgcaga
     5161 gcagcacgcg caggccgcgg ccggcggcat cgcgcgcgat cgccgcgccg ttgatgccac
     5221 cgccgatgac cagcaggtcg cagtccgcat ccaccgccat gcccgtgccc tcgcccgctt
     5281 cgtgcgggca ggctccatgc ccgggcggcc ggcggtcaag ccggcgccgc aaacgatgcg
     5341 ggccgggcgt gtttcgacgc ccggcccgca agaggcttca ggatggcccg cccggcagga
     5401 gcttcgccag cgccgccttc gcggccccgc acggccggca tcggtatcta cacaagtcag
     5461 cggttccacg ggcaggatga attgaccgat caatgctcgg gcgtcgatct cccctctccc
     5521 cttgcgggag aggggccggg ggagaggggg agacgccggc actgcgcccg gac
//
LOCUS       DAOQIE010000262         5564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8134_length_5564_cov_6.569432, whole genome shotgun sequence.
ACCESSION   DAOQIE010000262 DAOQIE010000000
VERSION     DAOQIE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8134_length_5564_cov_6.569432"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..3662
                     /locus_tag="PK144_14455"
     CDS             159..3662
                     /locus_tag="PK144_14455"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNF66974.1"
                     /translation="MEEFSGGIRRALAEAAGLALTPVAEHTACFAAWLNALRDDDYHV
                     SFSSLMLGVRLLGYDDWFEQQCEAALGPPPRMLATLFGGRLGERTLDTARACAASGHW
                     PDEDWPNAAEPAPVAGVPWPRRSGRGELSASAASLLRFAGNRARTRQATALDAIDLIA
                     AFVLDTHGHERQMESQGFELQRLRAAFAAREDEAALHGTGSGSGAPSSVAGAGAGADH
                     PLCRLRCDDETRYVLRHAVNLALGARRHAPPILTLRQILYALLEAGSGDGPVVTARGL
                     RASLDDFYRPGLGDEVAATGTQPDWPPPPFAAGEPLPEVWVEVTGVLDRAVRWCAGDP
                     LELPALALALVAEASDAATRAFAPLKPLTQAGIGERWLAALHAGAVTAGRDPSRYEQW
                     LYGEHRAAPSGVLPDLGRPRDAGDDCLDVRRYALALAATAVARDTAPPLAIGVFGDWG
                     AGKSHFMGLMELEMRTLAAGGDARFHRHVLPVWFNAWHYADANLWASLMQALLEAVAG
                     HFTAATEPGEDADFTRLLRRLDAASSGAALARTELAAAERSADEARKQFETARDQAQI
                     QECAVQARLADLFQELLAGQSGASIGKDLADELTALGFDELAKTAAAAGNRYDAILAT
                     VAQAETLTADARAFWLALRQWQWIDAGLLLAIAAGGGALIWALHAEATGQMLGAAMTA
                     SGAAAAALGWLRQRFGALGRLRERLAGIRQKQAEAAAKAALSEPARAAETARRDLEAA
                     RQRLDAALAARQQATAAVSESFVSARIARFVEQRLKSGLYREQLSLVSTIRRDLDTIA
                     RFLGQQSRERAQQEAQARERLAGLGLGHKATPALDRIVLYIDDLDRCPPERVVEVLEA
                     VHLLLAMPLFVVVVGVDVRWVSAALCRRYRGLLVGERGAAAGAHPTATPTDYLEKIFQ
                     IPFRLPAIDAVPARRLLGTLTGVSLLASGLRIPGPAVRVRPDASDAASRAAVPAGPDT
                     PAVVTERPHEPADAGAGAAGAEAPQAAEAQHEAGTADEARHTMIAVALSFSADEAGFM
                     ARLLATLGGSPRRVRRFVNSYRVYKAALRDAELRALTEGGGYREVLTALALQIGLNPH
                     LGRLREALLHRPGDDGQVQAQAPARVPEIGLDPAHRARLENALDALRADGDTPERERR
                     RLLDLLDRPDIARFSFAPAQR"
     assembly_gap    3664..3763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3764..4154
                     /locus_tag="PK144_14460"
     CDS             <3764..4154
                     /locus_tag="PK144_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66975.1"
                     /translation="AGKPLNLLPGAAFGLRMTTHPAGIAVVMACTHRSAARAPARSVR
                     PPALVHGLPGPMTGPVACAAASMRRRTVSEAPRTLPVPGPAGTRSGIRVRRVTAEGHR
                     PATMTAAHPLPWPFATIRQPGQPQSKE"
     gene            4164..5141
                     /locus_tag="PK144_14465"
     CDS             4164..5141
                     /locus_tag="PK144_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66976.1"
                     /translation="MSQIVSAVYATHADAEAARQALIVDGFEAAEVHITSLATGDDTT
                     PSAQGGGVRGFFRSLFGLEPEHPEVERYVAAVDSGRTVVSIEVDDDEALAARTVLLRY
                     GPVDLPPGEGYVTDADRLRNGDAESEYRTTMPFGESICAGAAGAAGEPGARPGTTPIP
                     ESETEARLATPPGLRESPALAPAVPPASVYEDPLLSEDPSRTQPFVPVVTQRVLPDPL
                     LDEPRPTDLYAARDASIETGERAADAVYSGAAHEREPVLAPDALDEGISHAPGLEETP
                     PTDPASVVGGSIAEDQHEPPHTVPPLAPIVPPVAPIAAPHQPERRRQPR"
     gene            5347..>5564
                     /locus_tag="PK144_14470"
     CDS             5347..>5564
                     /locus_tag="PK144_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66977.1"
                     /translation="MTAQSLGTVLLGAALIHYLILLAWFAVFTGAHDALHRLHGRWFR
                     LPVERFDAIHYAAMAGYKLGVLLFFLVPG"
BASE COUNT          694 a         2055 c         1981 g          734 t
ORIGIN      
        1 tccagcgtgt aggctgggtt aaggcattgc cttgcatagg ttattgcgtc ggcacccacg
       61 cttcgagcgt cgaatccgca ccgccggacg caaccgaatc cgccccgcca ccccgccccc
      121 tacactcggc catcgcccac agccccgcgg aggcagcgat ggaggagttc agtggtggca
      181 tccgccgggc gctcgccgag gccgccgggc tggcgctgac gccggtcgcc gaacatacgg
      241 cctgcttcgc cgcctggctg aacgcgctgc gcgacgacga ctatcacgtc tcgttcagca
      301 gcctgatgct cggcgtgcgc ctgctcggct acgacgactg gttcgagcag cagtgcgaag
      361 ccgccctcgg cccgccgccg cgcatgctgg cgacgctgtt cgggggccgg ctcggcgagc
      421 gcacgctcga taccgcgcgt gcctgcgcgg ccagcggtca ctggccggac gaggactggc
      481 cgaacgccgc cgagccggcg ccggtcgccg gcgtgccgtg gccgcggcgc agcggccgtg
      541 gcgagctgag cgcctcggcg gcgagcctgc tgcgcttcgc cggcaaccgc gcccgcacgc
      601 gtcaggccac ggcgctcgat gccatcgacc tgatcgccgc cttcgtgctc gacacccacg
      661 gccacgaacg gcagatggag agccagggct tcgagctgca gcggctgcgc gccgccttcg
      721 cggcgcgtga ggacgaagcg gcgctgcacg gcaccggcag cggcagcggg gcgccttcgt
      781 ctgtcgccgg cgccggcgcc ggcgctgacc atccgctgtg ccggctgcgc tgcgacgacg
      841 aaacccgcta cgtgctgcgc cacgcggtga atctcgcgct cggcgcccgc cggcacgcac
      901 cgccgatcct gacgctgcgc cagatcctgt acgcgctgct cgaagccggc agcggcgacg
      961 ggccggtggt gacggcgcgg ggcctgcgcg ccagtctcga tgacttctat cgccccggcc
     1021 tcggcgacga ggtcgccgcc accggcacgc agccggactg gccgccgccg ccgttcgcgg
     1081 ccggcgagcc gctgcccgag gtctgggtcg aggtcacggg cgtgctcgat cgcgccgtgc
     1141 gctggtgcgc tggcgatccg ctcgaactgc cggcgctggc actggcgctg gtcgcggaag
     1201 cctccgatgc ggccacccgc gccttcgcgc cgctcaaacc gctgacgcag gccggcatcg
     1261 gcgagcgctg gctggcggcg ctgcatgccg gggcggtcac ggccggtcgc gatccgtcgc
     1321 gctacgagca gtggctgtac ggcgagcacc gcgccgcgcc ctccggcgtg ctgcccgatc
     1381 tcggccgccc gcgcgatgcc ggcgacgact gcctcgacgt gcgccgctac gcgctggcgc
     1441 tggcggcgac cgcggtggcg cgcgacaccg caccgccgct ggcgatcggc gtgttcggcg
     1501 actggggcgc cggcaagagc cacttcatgg ggctgatgga gctggagatg cgcacgctcg
     1561 ctgccggcgg cgatgcgcgc ttccaccgcc atgtgctgcc ggtgtggttc aacgcctggc
     1621 attacgccga tgccaatctg tgggcgagcc tgatgcaggc gctgctggag gcggtcgccg
     1681 ggcatttcac ggcggccacg gagccgggcg aggacgccga tttcacccgg ctgctgcgcc
     1741 ggctcgatgc cgccagcagc ggggcggcgc tggcccgcac cgaactcgcc gcggccgagc
     1801 gcagcgccga tgaggccaga aagcagttcg agacggcccg cgatcaggcg cagattcagg
     1861 aatgcgccgt gcaggcccgg ctggccgacc tgtttcagga actgctcgcc ggccagagcg
     1921 gtgccagcat cggcaaggac ctcgccgatg agctgacggc gctcgggttc gacgaactgg
     1981 cgaagacggc cgcggccgcc ggcaatcgct atgacgcgat cctcgccacg gtggcgcagg
     2041 ccgaaaccct gaccgccgat gcccgcgcgt tctggctggc gctgcgccag tggcaatgga
     2101 tcgatgccgg cctgttgctg gcgatcgcag ccggcggcgg cgcgctgatc tgggcactgc
     2161 atgccgaggc cacggggcaa atgctcggtg ccgcgatgac ggcgagcggg gcggcggcgg
     2221 ccgcgctcgg ctggctgcgc cagcgcttcg gtgcgctggg ccgattgcgc gaacgtctgg
     2281 ccggcatccg gcagaagcag gccgaagccg ctgccaaggc cgcgctcagc gagccggcga
     2341 gggcggccga aaccgcccgg cgcgatctgg aggccgcgcg ccagcgcctc gatgcagccc
     2401 tcgccgcccg ccagcaggcc acggctgccg tgtcggaatc ctttgtttcg gcccgcatcg
     2461 cccgcttcgt cgagcagcgg ctgaagtccg ggctgtatcg cgagcagctc agtctggtct
     2521 cgacgatccg ccgcgacctc gacaccatcg cgcgcttcct cggccagcag tcccgggagc
     2581 gggcgcagca ggaggcgcag gcgcgcgaac gtctggcagg tctgggtctt ggccacaagg
     2641 ccacgccggc gctcgatcgc atcgtgctct acatcgacga cctcgaccgc tgcccgcccg
     2701 agcgcgtcgt cgaggtgctc gaagccgtgc atctgctgct ggcgatgccg ctgttcgtgg
     2761 tcgtggtcgg cgtcgatgtg cgctgggtat cggcggcgct gtgccggcgc taccgcggcc
     2821 tgctggtcgg cgagcgcggc gctgcggcgg gcgcgcaccc gaccgcgacg ccgaccgatt
     2881 acctcgaaaa gatcttccag atcccgttcc ggctgccggc catcgacgcc gtgccggccc
     2941 gccgcctgct cggcacgctg accggcgtgt cgctgctcgc cagcggcctg cgcatccccg
     3001 gcccggccgt gcgcgtgcgg ccggatgcga gcgatgcagc gtcccgtgct gcggtcccgg
     3061 ccggtcccga cacgccggcg gtcgtcacgg agcgtccgca cgagccggcg gatgcgggcg
     3121 ccggggctgc cggggccgaa gccccgcagg cagcggaggc gcagcacgag gccgggaccg
     3181 cggacgaggc gcgtcacacg atgatcgccg tcgcgctgag cttcagtgcc gacgaagccg
     3241 gcttcatggc ccggctgctg gccacgctcg gcggctcgcc gcggcgcgtg cgccgcttcg
     3301 tcaacagcta ccgcgtctac aaggcggcgc tgcgcgatgc cgagctgcgc gcgctgaccg
     3361 agggcggcgg ctaccgcgaa gtgctgacgg cgctggcgct gcagatcggc ctcaacccgc
     3421 atctcggccg cctgcgcgaa gccctgctgc accggcccgg ggacgacggg caggttcagg
     3481 ctcaggcgcc ggcgcgcgtg ccggagatcg ggctggaccc tgcccaccgc gcccggctcg
     3541 aaaacgccct cgacgcgctg agagccgacg gcgacacgcc cgagcgcgaa cgccggcgcc
     3601 tgctcgacct gctcgatcgc cccgacatcg cccgcttcag cttcgcaccg gcgcagcgct
     3661 gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccggc aagccgttga
     3781 accttctgcc cggcgcagcg ttcggattgc gcatgaccac acatccggca ggaatcgcgg
     3841 tcgtcatggc ctgcacgcac cgatccgcag cgcgtgctcc ggcccgcagc gtccgcccgc
     3901 ctgcccttgt ccacggtctg ccggggccga tgaccggacc cgtcgcctgc gccgccgcct
     3961 cgatgcggcg gcgcaccgtc agcgaagcgc cgcgcacgct gcccgtcccg gggccggccg
     4021 gaacgcgcag cggcattcgc gtccgcaggg tcacggccga gggccaccga cccgccacca
     4081 tgactgccgc gcacccgttg ccgtggccct tcgccacgat ccgtcagccg gggcagccgc
     4141 agtcgaagga gtgaggattc gtcatgagcc agatcgtttc tgccgtgtat gccacccacg
     4201 ccgatgccga ggccgcgcgt caggcgctga tcgtcgacgg cttcgaggcc gccgaggtcc
     4261 acatcacctc gctcgccacc ggcgacgaca ccaccccgtc cgcacaaggc ggcggtgtgc
     4321 ggggtttctt ccgttcgctg ttcggactcg aaccggagca tccggaggtc gaacgctacg
     4381 tcgcggccgt cgacagcggc cgtaccgtcg tcagcatcga ggtcgatgac gacgaggcgc
     4441 tcgcggcgcg cacggtgctg ctgcgctacg ggccggtcga cctgccgccc ggcgagggct
     4501 acgtgaccga tgccgaccgg ctgcgcaacg gcgatgccga gtccgagtac cgcacgacga
     4561 tgccgttcgg cgagagcatc tgcgccggcg cggccggggc ggccggcgag ccgggcgcgc
     4621 gcccgggcac gacgccgatc cccgagtccg aaaccgaggc gcggctcgcg accccgcccg
     4681 gcctgcgcga atccccggcc ctggcgccgg ccgtgccgcc ggcttcggtc tatgaggatc
     4741 cgctgctgag cgaggacccg tcgcgcacgc agcccttcgt gccggtcgtg acgcagcgcg
     4801 tgctgccgga cccgctgctc gacgagccgc gcccgaccga cctgtacgcg gcgcgcgatg
     4861 cgtcgatcga gaccggcgag cgggccgcgg atgccgtcta cagcggcgcg gcgcacgagc
     4921 gcgagccggt gctcgcgccc gatgcgctcg atgagggcat cagccacgcg ccggggctcg
     4981 aagaaacgcc gccgaccgat ccggcgagcg tggtcggggg ctcgatcgcc gaggaccagc
     5041 atgaaccgcc gcacacggtg ccgccgctgg cgccgatcgt gccgccggtc gcgccgatcg
     5101 cagcgccgca ccagccggaa cgtcgccgcc agccgcgctg agcgatctgc agggggcctg
     5161 catggggcct gatgtctggt ttgcgtgcgt tccgcttccg gtcgatgcgc gcgtgccgcg
     5221 acggccggtt ccgcggaacg ggtgggcaac ggcgaccctg cggggccggt cgccgttgtc
     5281 tgccgatccg ggccgcctgc aaccgcagcg gcctgacccg gccccgcgtc gaccggagcc
     5341 caggccatga ccgcccagtc cctcggcacc gtgctgctcg gcgcggcgct gatccactac
     5401 ctgatcctgc tcgcgtggtt cgccgtgttc accggcgccc acgacgcgct gcaccgcctg
     5461 cacggccgct ggttccggct gccggtcgag cgcttcgacg ccatccatta cgcggcgatg
     5521 gccggctaca agctcggcgt gctgctgttc tttctggtgc cggg
//
LOCUS       DAOQIE010000263         5550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8167_length_5550_cov_5.070974, whole genome shotgun sequence.
ACCESSION   DAOQIE010000263 DAOQIE010000000
VERSION     DAOQIE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_5550_cov_5.070974"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..374)
                     /locus_tag="PK144_14475"
     CDS             complement(105..374)
                     /locus_tag="PK144_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF66978.1"
                     /translation="MAWKIELAPAALRALKQLDPPVARRLLSFLHQRLAPLDDPRSLG
                     AALQGARFEGLWRYRVGDYRIIARLQDDTLHILVVHIGHRRDVYR"
     gene            complement(358..582)
                     /locus_tag="PK144_14480"
     CDS             complement(358..582)
                     /locus_tag="PK144_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HNF66979.1"
                     /translation="MLTIELPAELEARLEALASEHGQSAGALVREAVLKLLDDLEDIA
                     AAEAALAEIEAGHGQPVPLEDVVKAFGLED"
     gene            complement(768..1571)
                     /locus_tag="PK144_14485"
     CDS             complement(768..1571)
                     /locus_tag="PK144_14485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF66980.1"
                     /translation="MSAAAGTALITGASSGIGAVYAERLAGRGHDLILVARNVERLDA
                     LAGLLRTRHGVQVRTMSADLGTPAGVTSVEAVLRSDESITLLVNNAGFGSTAPLLQSD
                     PASMTQMIDVNVTALTRLTQAIAPGFVARGGGTIINIASIVAIGPELLNGVYGATKAY
                     VLALSQSLHKELAAHGVRVQVVLPGATATRFWDIAGLPVEHLPEAWVMSASDLVDAAL
                     IGLDRGEFATLPSLMDESEYTAWEAARQSMLGHLSNASPAERYRLTDRG"
     gene            1693..2637
                     /locus_tag="PK144_14490"
     CDS             1693..2637
                     /locus_tag="PK144_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNF66981.1"
                     /translation="MRTIGFVLADGFQVMSLAALSVFELANASLGDAGYALHLHSAGG
                     GPVRSSLGFGIDTRAFGPDSPDTIIVTGALVPDPAPAGVLDYLRDTGRGARRIAGICT
                     GAFVLAEAGLLDGRRATTHWAHAAALQQRHPAIRVEADRIFCADQGVWTSAGMSAGID
                     LALALLEDDHGPALAKSVAHKLVVYHRRAGGQSQFSTLVELAPKSDRIRQALAWAREH
                     LAEPLTVETLAAAVHLSPRQFSRVFREETGQSPAKAVERLRIEAARAMIEDGRHGLAV
                     VARATGFGDRERLRRAFLRGYGQPPQALRRRNRDAQPA"
     gene            complement(2689..3378)
                     /locus_tag="PK144_14495"
     CDS             complement(2689..3378)
                     /locus_tag="PK144_14495"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HNF66982.1"
                     /translation="MSTDHPDTDADTLAPHWVVTGGMLLAGSAGFVNTIMLSFFHLPV
                     SHMSGSVSKIGIDLVNGNADDLQAFLGIVGGFLIGAFLSGVIIGNRNLRPGRRYGHSL
                     LIQGTLLMIATFLAQYGYLIAVPLAATACGMQNAMASSYRGLTLRTTHISGVVTDFGI
                     HLGHLVRERRVRDPWKLVLLAGLLGAFFTGGALGMLGIRESGLGMLYLAAFGNLFAGM
                     LYLIVYPHAPL"
     gene            complement(3502..3981)
                     /locus_tag="PK144_14500"
     CDS             complement(3502..3981)
                     /locus_tag="PK144_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66983.1"
                     /translation="MSAIVLLDTSVYLNVLDVPGFNQDKDEVFAAFETSIRNSDYFLL
                     PLATVWETGNHIAALGDGQTRRRYAERLLDDVTKAFNGQAPYQATHFPDRNEFLAWLA
                     DFPDMAMRNKSARKQREGTSLADLSIIKEWQRSCERHSMSRVRIWSLDSDLAGYDRQ"
     gene            complement(3978..5324)
                     /locus_tag="PK144_14505"
     CDS             complement(3978..5324)
                     /locus_tag="PK144_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF66984.1"
                     /translation="MLTAILLENFKSYRHRARLSLAPLTVLIGANASGKSNAIEGMRL
                     LSWLAQGQKLSAIQYAVQSGERVVRGTIDDLSFERGEDFRLGAETDAPKWNSLSMTLA
                     RRADGLHIIGETLTHAGERVPLYMLDQPSHERSNDASVAYNNFARGGVKPHVTCSDQT
                     AIFTQLTSPATFHSENKSSRERIPPVVRNLEQCLSAMLFLDPVPARMRDYAFPSDRVL
                     QGDGSNLSAVLHNLWCAAQDQDAESEEDADSCQSQRQTILAFIQSLPEQNIAGISFLK
                     EPRGGVMIQLTETFGNQTRDYDASLLSDGTLRVLSIAAAMLSAPAGSLVVIEEIDNGV
                     HPNRARHLLENIRDIAERRGLRVLLSPHNPAMLDALPDSALPDVLFCYRDPQDGSSRV
                     TRLQDVPDYPELIAQDSLGGLVTSGALDRFVKQPRSLDRQALALKWLENVKARSGQ"
BASE COUNT          888 a         1810 c         1941 g          911 t
ORIGIN      
        1 aggcgacgcg catcgtcgcg ctcgccgagg cgattgcggc gcgtgatgcg ctggcggcga
       61 acgacgcgca gggtgcggac gaaaccgtgc cgtgaacggc gggctcagcg gtagacgtcg
      121 cgccgatgac cgatgtggac gacgaggatg tgcagggtgt cgtcctgcag gcgggcgatg
      181 atccggtaat cgccgacccg gtagcgccag agaccctcga agcgcgctcc ctgcagggca
      241 gcgcccaagc tgcgcggatc gtcgagcggt gcaaggcgct ggtgcaggaa gctcaggagg
      301 cggcgtgcga cgggcggatc gagctgtttc agcgccctga gagccgccgg cgccagttca
      361 atcttccagg ccaaacgctt tcaccacgtc ttcgagcggg accggctggc cgtgaccggc
      421 ttcgatctcg gccagggcgg cctcggccgc cgcgatgtct tccagatcgt cgagcagctt
      481 cagcaccgcc tcccggacca gggctccggc gctctggccg tgctcgctgg ccagcgcttc
      541 cagccgggct tcgagttcgg ccggcagttc gatcgtcagc atcgtgtacc tccgggtcag
      601 cggccgggtg gcgttcgtgc cgggatggca agaccccggc gcccatgcgc gggaaaccgg
      661 gctgactata tccccgtccg ggccctgcgt gcggagggtg gaggcagcac gacacccccc
      721 gcgcagcgcc tgccggaggt cactgtgcag gcatccgccg tctgcgctca gccccggtcc
      781 gtcagccggt agcgctcggc cggactcgcg ttcgacagat ggccgagcat cgactggcgc
      841 gcggcttccc aggccgtgta ctcggattcg tccatcagcg agggcagcgt cgcgaactcg
      901 ccgcgatcca gtccgatcag cgcggcatcg accagatcgg aggccgacat cacccaggct
      961 tccggcagat gctcgaccgg caggccggcg atgtcccaga agcgggtggc ggtcgccccc
     1021 ggcagcacca cctgcacgcg cacgccgtgc gcggccagct ccttgtgcag cgactggctg
     1081 agggccagca cgtaggcctt ggtcgcgccg tagacgccgt tcagcagctc cggcccgatc
     1141 gcgacgatcg aggcgatgtt gatgatcgtg ccgccgccgc gcgcgacgaa gcccggcgcg
     1201 atggcctggg tcaggcgggt cagcgccgtg acgttcacgt cgatcatctg cgtcatgctc
     1261 gccggatcgg actgcagcag cggcgcggtg ctgccgaagc cggcgttgtt gaccagcagc
     1321 gtgatcgact cgtcactgcg cagcaccgcc tcgaccgacg tcacgccggc cggcgtgccg
     1381 agatcggcgc tcatggtgcg cacctgcacg ccgtggcgcg tgcgcagcag cccggccagc
     1441 gcgtcgagac gttcgacgtt gcgggcgacg aggatcaggt cgtggccgcg gccggcgagg
     1501 cgctcggcat agacggcgcc gatgccggag gaggcgccgg tgatgagggc agtacccgct
     1561 gctgcggaca tggtcggagg ctccggtgct gcgccggtcc gggagcggac ggcgtcggcc
     1621 ctcagcatcc cgcgccgcgc cgatgtcgca aatgtcgtat aaacatcgaa aacggacatc
     1681 gaggttgcgg cgatgcgcac gatcggtttt gtgctggcag acggtttcca ggtcatgtcg
     1741 ctcgcggcgc tgtcggtgtt cgagctggcg aacgccagcc tcggcgacgc cggctacgcg
     1801 ctgcacctgc actcggccgg cggtggcccc gtgcgcagct cgctcggctt cggcatcgat
     1861 acccgggcat tcgggcccga cagccccgac accatcatcg tcaccggcgc gctggtgccg
     1921 gacccggcac cggcgggcgt gctcgactac ctgcgcgaca ccggccgcgg cgcccggcgc
     1981 atcgccggca tctgcaccgg cgccttcgtg ctcgccgagg ccgggttgct cgacggccgg
     2041 cgcgcaacca cgcactgggc gcatgccgcc gcgctgcagc agcgccatcc ggcgatccgc
     2101 gtcgaggccg accgcatctt ctgcgccgac cagggcgtgt ggacctcggc cggcatgagc
     2161 gccggcatcg atctggcgct ggcgctgctg gaggacgacc acggccccgc cctcgcgaag
     2221 tcggtcgcgc acaagctcgt cgtctatcac cgccgggccg gcggccagtc gcagttctcg
     2281 acgctggtcg agctggcgcc gaaatccgac cgcatccgcc aggcgctcgc ctgggcccgc
     2341 gaacacctcg ccgagccgct gacggtcgag accctcgccg ccgccgtcca tctgagtccg
     2401 cgccagttca gccgggtgtt tcgcgaggag accggccagt cgccggcgaa ggcggtcgag
     2461 cgcctgcgca tcgaggcggc gcgcgcgatg atcgaggacg gtcgccacgg gctggcggtc
     2521 gtggcacgcg ccaccggctt cggcgaccgc gagcgcctgc gccgggcctt cctgcgcggc
     2581 tacggccagc cgccgcaggc gctgcgccgg cgcaaccggg acgcgcagcc ggcctgaagt
     2641 cccggccgct gccgcagccc ggcccgctgc gggtccggcc gccggcgcct acagcggggc
     2701 gtgcggatag acgatcaggt acagcatgcc cgcgaacagg ttgccgaagg ccgcgaggta
     2761 cagcatgccg aggccggatt cgcggatgcc gagcatgccg agcgcaccac cggtgaagaa
     2821 ggcgccgagc agcccggcga gcagcacgag cttccacgga tcgcgcaccc ggcgctcgcg
     2881 cacgaggtgg ccgagatgga tgccgaagtc ggtgacgacg ccactgatgt gcgtggtccg
     2941 cagcgtcagg ccgcggtagc tgctcgccat cgcgttctgc atgccgcagg cggtcgcggc
     3001 cagcggcacc gcgatcaggt agccgtactg cgcgagaaag gtcgcgatca tcagcagcgt
     3061 tccctggatc agcagcgaat gtccgtagcg gcggccgggg cgcaggttgc ggttgccgat
     3121 gatgacgccg gacaggaagg cgccgatcag gaagccgccg acgatgccga ggaaggcctg
     3181 caggtcatcg gcgttgccgt tgacgaggtc gatgccgatc ttcgagacgc tgccgctcat
     3241 gtgcgagacc ggcaggtgga agaagctcag catgatcgtg ttgacgaagc ccgccgagcc
     3301 ggcgagcagc atgccgccgg tgacgaccca gtgcggggcg agcgtgtcgg cgtcggtgtc
     3361 ggggtgatcg gtgctcatcg gctagggtgg cggtggcgcg gctccggagt gacccggaca
     3421 gtcgcagaag tccgcgagtg tagggacggc ggcggcgggg accaagccgg gtgtggcggt
     3481 ggccgggttc gtactggatc gctactggcg gtcgtacccc gccagatcgc tgtcgagcga
     3541 ccagatccgg acgcggctca tggaatggcg ctcacaggaa cgctgccatt ccttgatgat
     3601 ggacagatcg gcgaggctgg tgccttcacg ctgtttgcgg gcggatttgt tgcgcatggc
     3661 catgtccgga aaatcggcca gccatgcaag aaactcgttg cgatccggaa agtgcgtggc
     3721 ctgataaggg gcttgtccat tgaaagcctt cgtcacgtcg tccagcaggc gttccgcata
     3781 gcgtcggcgc gtctggccgt cacccaaggc ggcgatgtgg ttgccggttt cccacacggt
     3841 cgccagcggc agaaggaaat agtcgctatt ccggatggag gtttcaaacg ctgcaaacac
     3901 ctcgtccttg tcctgattga atcccggtac atccagcacg ttcagataca cggacgtgtc
     3961 cagcaggacg atcgcactca ttgtccgctt ctcgccttca cattctccag ccatttcagt
     4021 gcaagtgcct gacgatccag cgaccttggc tgcttgacga agcggtcgag cgcgccactc
     4081 gtgaccaggc cgccgaggga atcctgcgcg atcagttccg gatagtccgg tacatcctgc
     4141 aggcgggtca cgcggctgga gccgtcctgc gggtcgcggt aacagaacag cacgtccggc
     4201 agtgccgagt ctggcaaggc gtcgagcatg gcggggttat ggggggacag cagcacccgc
     4261 agtccgcgac gctcggcgat gtcgcggata ttctccagca gatgccgggc gcgattcgga
     4321 tgcacgccgt tatcgatttc ctcgatcacc accaggctgc ctgcgggggc agagagcatg
     4381 gcggccgcaa tggacaggac ccgcagcgtg ccgtcggaca gcagcgaggc atcgtagtcg
     4441 cgggtctggt tgccgaaggt ttcggtcaac tggatcatga ccccgccacg cggttccttc
     4501 agaaagctga tgccggcaat gttctgctcg ggcaggcttt gaatgaacgc cagaatggtc
     4561 tggcgctggc tctggcagga atcggcatct tcttccgatt cggcatcctg gtcctgcgcc
     4621 gcgcaccaga gattgtggag aaccgctgaa aggttgctgc cgtcgccctg caatacgcga
     4681 tcggacggaa atgcgtaatc ccgcatgcgg gcgggaaccg ggtccagaaa cagcatggca
     4741 gacaggcact gttccagatt tctgaccact ggcggaatcc gttcgcgcga tgacttgttt
     4801 tcggagtgaa aggtcgcggg gctggtcaac tgggtgaaga tcgcggtctg gtcgctgcag
     4861 gttacgtgcg gcttgacgcc gccgcgggca aagttgttgt aagccaccga ggcgtcattg
     4921 ctgcgctcgt gcgagggctg gtcgagcatg tacagcggca ctctttcgcc cgcgtgcgtc
     4981 agggtttcgc caatgatgtg caagccatcg gcccggcgtg ccagcgtcat cgacaggctg
     5041 ttccacttcg gcgcgtccgt ttccgcgccc agcctgaaat cttcgccccg ctcgaaagac
     5101 aggtcatcaa tggtgccgcg caccacgcgc tccccgcttt gcacggcata ttgaatggca
     5161 gaaagctttt gtccctgtgc cagccaggac agcaggcgca tgccttcgat ggcgttgctt
     5221 ttgcccgaag cgttggcgcc gatcaatacg gtcaatggcg ccagtgacag tctggcgcga
     5281 tgccggtagc tcttgaagtt ttcgagcagg atggcggtca gcatggcagg ctctcctttt
     5341 gatgagtcag ggatgacgac gcctgtccat tcgtcggttg gcccagtcgg gtagggtgcg
     5401 cattgatgta aacgcacgag gcgccgcaac gcggaaggcg gattacggcg cgcggtaaaa
     5461 tgtcgatgtg ccttgctgtg tagcccaatg cgtgcctaat ccgccctacg cttgctgcaa
     5521 gcgatggcct tccttcatca ccatatccca
//
LOCUS       DAOQIE010000264         5458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8406_length_5458_cov_5.891172, whole genome shotgun sequence.
ACCESSION   DAOQIE010000264 DAOQIE010000000
VERSION     DAOQIE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8406_length_5458_cov_5.891172"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="PK144_14510"
     CDS             <1..1769
                     /locus_tag="PK144_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNF66985.1"
                     /translation="GVQIDPAGAERIATAAVAGGHVQHIVLADGSGRVLAEASGPKLE
                     SPVPEWFSAIAALRPAVASIGIADDGRPIGKVTVEAAGLPALAELWATARRISDALLV
                     LGLISAALGVLVLRRQLAPLERVAEQADAILHNRFEPSGLRPSAPELRRVVEAMDRLV
                     GRMKEQFEEEIARSERLRREALSDQVTGLPNRRWFDHHLQDVLAADDESAHGALLLLR
                     VTDLDGRNRRHGYVRTDQWLRELAGALTALGRAREGWALARLRGADFVVLAPGVDAAG
                     IETFGAGLITALASLADGDAPSPVRCGLTPYQRGDSPATLLAQADAALSAAEAAGTDW
                     QLPPAVELADAAPGHDWAALLRTALQEERFVLYGQPALAADGSVLHHEVLARLPLADA
                     DEPLVAGTFVPIAARLGLAADLDRLIVLLALDQIDRERRPLAVNLSEAALRDRGFLDF
                     FAIELGQRPALAPWLIFETREAYVLHDLDAATAFIAVLRELGCRFGLDHVGRSFAPAG
                     YLARLGVEYLKLDGGFVRGVDREPVQRQYVEALCRSAHALGISVIAERVESETEAADL
                     RALGFDGLQGRALAAPAPLGRA"
     assembly_gap    1832..1931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1963..2400
                     /locus_tag="PK144_14515"
     CDS             1963..2400
                     /locus_tag="PK144_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66986.1"
                     /translation="MPLLLPFLPAFIAGALAVLVFHQSALGLLHLVGLTPRTPFVLDG
                     VPPLGVPQLLSLMFWGGVWGLALLPLLRPLGDARCWLRGLVLGALGPTLVSWFVVAPL
                     KGLPLAGGWQPAAMATGLIVNAAWGLGLVALLRLAQRAGIPGG"
     gene            2558..>3756
                     /gene="nirJ"
                     /locus_tag="PK144_14520"
     CDS             2558..>3756
                     /gene="nirJ"
                     /locus_tag="PK144_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HNF66987.1"
                     /translation="MFRISHYLHALRTPGAATGPVRRPPGPVVIWNLVRRCNLACRHC
                     YSISADIDFPGELTTAEVLATLDDLQRFRVPALILSGGEPLLRPDIHEISAYAKALGL
                     YLGLSTNGTLIDADNIGRIAATGFDYVGISLDGLRTTHDRFRRLDGAFDRSLAGIRLC
                     RTHGIKVGLRCTLTQDNHAQLPALLELMRTEQVDKFYLSHLNYAGRGARNRRDDAVHA
                     MTRAAMDLLFETAWDDLDSGREYVSGNNDADGPYLLQWAQARGFDTTQLEQRLVQWGG
                     NRSGIDIANIDNTGEVHPDTFWWNHPLGNVREQPFSTIWSTTRDPLMLGLRSEPRPLK
                     GRCAACRHLPICGGNTRVRAFQTSGDPWAEDPGCYLDDDEIGLPGGYAGEAAQPGPRA
                     APIRFDRR"
     assembly_gap    3757..3856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3894..>5458
                     /locus_tag="PK144_14525"
     CDS             3894..>5458
                     /locus_tag="PK144_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNF66988.1"
                     /translation="MRPSLSLLPALLLASLSAAPAAPAAAQPSDAASAGPAPALYAQH
                     CASCHGATRLGGMGPALLPENLGRLKPAEALATIRDGRAATQMPAFAAKLDAAQLQAL
                     ADFVYTPPAVAPVWGIDEIRASRTFDAEAARLPARPVYDADPRNLFVVVELGDHHVTV
                     LDGDRFRPLTRFASRFALHGGPKFSPEGRFVYFCSRDGWVTKYDLWNLRTVAEVRAGI
                     NARNLAVSDDGRFVMVANYLPQTLVLLSADELAPLKLYPVADAAGHGSRVSAVYTAAP
                     RGSFVAALKDVQEVWEIPYRDDLPPVHAGPMQDDRAGAAGRSAALEPRRIALDDVLDD
                     FFFDPAYRHLIGASRDGGKGQVIDLDAGTRVATLDLPGMPHLGSGITWEWQGRPVLAT
                     PNLKESAITVIDMTNWQVIKRIPTLGPSFFMRSHAQLDTAWIDVSLGRERDAMQLIDK
                     NTLEIRTTIRPQPGRTAAHVEFTQDGRQALISIRERDGALVVLDTQSLTEITRLPMAM
                     PSGKYNVHNKITR"
BASE COUNT          721 a         2008 c         1786 g          743 t
ORIGIN      
        1 tcggcgtgca gatcgacccg gccggcgccg agcgcatcgc gaccgcggcc gtcgccggcg
       61 gccacgtgca gcacatcgtg ctcgccgacg gcagcggcag ggtgctcgcc gaggcctcgg
      121 ggccgaagct cgaatcgccg gtgccggaat ggttcagcgc gatcgcggcg ctgcgcccgg
      181 ccgttgccag catcggcatc gccgacgacg gccggcccat cggcaaggtc acggtcgagg
      241 ccgccggcct gccggcgctc gccgagctgt gggcgacggc acggcgcatc agcgatgcgc
      301 tgctggtgct cgggctgatc agcgccgcac tcggcgtgct cgtgctgcgc cgccagctcg
      361 cgccgctcga acgcgtcgcc gagcaggccg atgccatcct gcacaaccgc ttcgagccga
      421 gcggcctgcg accgtcggcg ccggagctgc gccgcgtcgt cgaggccatg gaccggctgg
      481 tcggacgcat gaaggagcag ttcgaggagg agatcgcgcg cagcgagcgc ctgcgccgcg
      541 aagccctcag cgatcaggtc accggcctgc cgaaccgccg ctggttcgat caccacctgc
      601 aggacgtgct cgccgccgat gacgaatccg cgcatggtgc gctgctgctg ctgcgcgtga
      661 ccgacctcga cggccgcaac cgccgccacg gctacgtgcg caccgaccag tggctgcgcg
      721 agctggccgg ggcgctgact gcgctcggtc gcgcacgcga gggctgggcg ctcgcgcggc
      781 tgcgcggcgc cgatttcgtc gtgctcgccc ccggcgtcga tgccgccggc atcgagacct
      841 tcggtgccgg cctgatcacg gcgctcgcga gcctcgccga tggcgacgcc ccctccccgg
      901 tgcgctgcgg gctgacgccg taccagcgcg gcgacagccc ggccacgctg ctcgcgcagg
      961 ccgatgcggc gctgtcggcg gccgaggccg ccggcacgga ctggcagctg ccgccggccg
     1021 tcgaactcgc cgacgccgcc cccggccacg actgggccgc gctgctgcgc acggcgctgc
     1081 aggaagaacg cttcgtgctc tacggccagc cggcactggc cgccgatggc agcgtgctgc
     1141 accacgaagt gctcgcgcgc ctgccgcttg ccgatgccga cgagccgctg gtggccggca
     1201 ccttcgtgcc gatcgctgca cgactcgggc tggcggccga tctcgaccgg ctgatcgtgc
     1261 tgctcgcgct cgaccagatc gaccgcgagc gccggccgct ggccgtgaac ctgtcggagg
     1321 cggcgctgcg cgaccgcggc ttcctggact tcttcgcgat cgaactcggt cagcgcccgg
     1381 cgctcgcgcc gtggctgatc ttcgagacgc gcgaggccta cgtgctgcac gacctcgacg
     1441 cggcgacggc cttcatcgcg gtgctgcgcg aactcggctg ccgcttcggc ctcgaccacg
     1501 tcgggcgcag cttcgcgccg gccggctatc tggcgcggct cggcgtcgag tacctgaagc
     1561 tcgacggcgg cttcgtgcgc ggcgtcgatc gcgagccggt gcagcgccag tacgtcgagg
     1621 cactctgccg cagcgcccac gcgctcggca tttcggtgat cgccgagcgc gtcgaatccg
     1681 aaaccgaggc cgccgacctg cgcgcgctcg gcttcgacgg cctgcagggc cgggcactgg
     1741 cagcgccggc accgctgggc agagcctgat ccaggcccgg ccggataccc cggcatcgcc
     1801 tcgacatcgc ttcgggatgg ccgtttgccc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ngccggtccc atcccgaaag gagtcgatcg cgatgccctt gctgctgcct
     1981 ttcctgccgg ccttcatcgc cggggcgctg gccgtgctgg tgttccacca gagcgcgctc
     2041 ggcctgctgc atctcgtcgg gctgaccccg cgcacgccgt tcgtgctgga cggcgtgcca
     2101 ccgctcggcg tgccgcaact gctgtcgctg atgttctggg gcggcgtctg ggggctcgcg
     2161 ctcctgccgc tgctgcgccc gctcggcgat gcccggtgct ggctgcgcgg cctcgtgctc
     2221 ggcgcgctcg gtccgacgct ggtgtcgtgg ttcgtcgtcg cgccgctgaa gggcctgccg
     2281 cttgccggcg gctggcagcc ggcggcgatg gcgaccgggc tgatcgtcaa cgctgcgtgg
     2341 gggctcggct tggtcgcgct gcttcggctc gcgcaacgcg ccggcatccc cggcggctga
     2401 agcgcctgca gccggggccg ccctaccacc ggcggcggtt tacctacccc gcacgaggga
     2461 gccggacgcg cgcaagcact tgtcggaaat caagtgccgg tcccgggcgg cgggtgacga
     2521 tgcccggcac gtacccgccg cgacccggac tgccgccatg ttccgcatca gccactacct
     2581 gcacgccctg cgcacgcccg gggccgccac cgggccggtg cgccgtccgc ccgggccggt
     2641 cgtgatctgg aacctcgtgc gccgctgcaa cctcgcctgc cggcactgct actcgatctc
     2701 ggccgacatc gacttccccg gtgaactgac gacggccgag gtgctggcaa cgctcgacga
     2761 cctgcagcgc ttccgcgtgc cggcgctgat cctctccggc ggcgaaccgc tgctgcgccc
     2821 cgacattcac gaaatctccg catacgccaa ggcactcggc ctgtacctgg ggctttcgac
     2881 caacggcacg ctgatcgatg cggacaacat cgggcgcatc gccgcgaccg gcttcgatta
     2941 cgtcggcatc tcgctcgatg gcctgcgcac cacgcacgac cgcttccgcc ggctcgacgg
     3001 ggccttcgac cgctcgctgg ccggcatccg gctctgccgc acacacggca tcaaggtcgg
     3061 gctgcgctgc acgctgacgc aggacaacca cgcgcaactg ccggcgctgc tggagctgat
     3121 gcgcaccgaa caggtcgaca agttctacct gtcgcacctg aactacgccg gccgcggcgc
     3181 gcgcaaccgc agggatgacg ccgtgcatgc gatgacgcgc gcggcgatgg acctgctgtt
     3241 cgagaccgcc tgggacgacc tcgacagcgg gcgcgaatac gtcagcggca acaacgacgc
     3301 cgatggcccc tatctgctgc agtgggcgca ggcgcgcggc ttcgacacca cgcagctcga
     3361 acagcgcctc gtgcagtggg gcggcaaccg ctcgggcatc gacatcgcca acatcgacaa
     3421 caccggcgag gtgcatccgg acaccttctg gtggaaccac ccgctcggca acgtccgcga
     3481 acagccgttc tcgacgatct ggtcgacgac gcgcgatccg ctgatgctcg gcctgcgcag
     3541 cgagccgcga ccgctgaagg gccgctgcgc cgcctgcaga caccttccga tctgcggcgg
     3601 caacacgcgc gtgcgcgcct tccagaccag cggcgatccc tgggccgaag acccgggctg
     3661 ctacctcgat gacgacgaga tcggcctgcc gggcggctac gccggcgagg cagcgcagcc
     3721 cggcccgcgc gccgccccga tccgcttcga ccgccgnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnngcga ccgccaccgg tcgccgaacc ggaaaccgcc gtcatgcgcc
     3901 cgagcctgtc cctgctgccc gccctgctgc tcgccagcct gagcgccgcc cctgcggccc
     3961 cggccgcggc gcagccatca gatgccgcct ccgccggccc ggcaccggcg ctgtacgcgc
     4021 agcactgcgc gagctgccac ggggcgacgc gcctcggcgg catgggtccg gcgctgctgc
     4081 cggagaacct cgggcggctg aagccggccg aggcgctggc gacgatccgc gacggccgcg
     4141 ccgccacgca gatgccggcc tttgcggcga agctcgacgc cgcgcagctg caggccctcg
     4201 ccgatttcgt ctacacgccg ccggcggtcg cgccggtctg gggaatcgac gagatccgcg
     4261 caagccgcac cttcgatgcc gaagccgcgc gcctgccggc cagaccggtg tacgacgccg
     4321 atccgcgcaa tctcttcgtc gtcgtcgaac tcggcgatca ccacgtcacc gtgctcgacg
     4381 gcgaccgctt ccggccgctc acgcgcttcg catcgcgctt cgcgctgcac ggcggcccga
     4441 agttctcgcc cgagggccgc ttcgtgtact tctgctcgcg cgacggctgg gtcacgaagt
     4501 acgacctctg gaacctgcgc accgtcgccg aggtgcgtgc cggcatcaac gcccgcaacc
     4561 tcgccgtcag cgatgatggc cgcttcgtga tggtcgccaa ctacctgccg cagacactgg
     4621 tgctgttgtc ggccgacgag ctcgcgccgc tgaagctcta cccggtcgcc gatgccgccg
     4681 gccacggctc gcgggtcagc gccgtataca cggccgcacc gcgcggcagc ttcgttgcgg
     4741 cgctgaagga cgtgcaggag gtctgggaaa tcccgtaccg cgacgatctg ccgccggtcc
     4801 acgccggccc gatgcaggac gatcgcgcag gcgccgccgg ccgcagcgcc gcgctggagc
     4861 cacgccgcat cgcgctcgat gacgtgctcg atgacttctt cttcgatccc gcctaccgcc
     4921 acctgatcgg cgcctcgcgc gacggcggca agggtcaggt catcgacctc gatgccggca
     4981 ccagggtcgc aacgctcgat ctgcccggca tgccgcatct cggttccggc atcacctggg
     5041 aatggcaggg ccggccggtg ctggcgacgc cgaacctgaa ggaatcggcg atcaccgtca
     5101 tcgacatgac caactggcag gtcatcaagc gcatcccgac cctcggcccg agcttcttca
     5161 tgcgcagcca cgcgcagctc gacaccgcct ggatcgatgt ctcgctcgga cgcgagcgcg
     5221 acgcgatgca gctgatcgac aagaacacgc tggagatccg cacgaccatc cgcccgcagc
     5281 cgggccgcac cgccgcacac gtcgagttca cgcaggacgg ccgccaggcg ctcatcagca
     5341 tccgcgaacg cgacggcgcg ctggtcgtgc tcgacacgca gagcctcacc gagatcaccc
     5401 ggctgccgat ggcgatgccc tcgggcaagt acaacgtgca taacaagatc acgcgcga
//
LOCUS       DAOQIE010000265         5443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8446_length_5443_cov_6.257610, whole genome shotgun sequence.
ACCESSION   DAOQIE010000265 DAOQIE010000000
VERSION     DAOQIE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8446_length_5443_cov_6.257610"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK144_14530"
     CDS             complement(<1..1452)
                     /locus_tag="PK144_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF66989.1"
                     /translation="MSDPLLRSLGLSPDALRDGDLIARSPIDGTVLARVRSHTPADID
                     AAIARAHLAFHRWRTVPAPRRGELVRLLGEELRANKAALGALVTLETGKIAAEGEGEV
                     QEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVTGVISAFNFPVAVWAWNF
                     ALALVCGNPVVWKPSEKTPLSALACQALFRRALLRWGEAPEGLSELLIGARAAGEQLA
                     DDARVALVSATGSTAMGRVLGPRVAARFGRTLLELGGNNAVIVAPSADLELALRGIVF
                     GAIGTAGQRCTSTRRLIVHTSIHDALLERLQAAFVSARVGDPRQAGTLVGPLIDEAAF
                     TAMQSALAAAVAEGGRLVCGGERVLKVRMPEAFYVTPALLTMPAQSAAVRRETFAPIL
                     YVLRYRTLDEAIALNNDVPQGLSSAIFTNDLREAGQFLGATGSDCGIANVNLGTSGAE
                     IGGAFGGDKETGGGRESGSDAWKSYMRRATNTLN"
     gene            complement(1486..3156)
                     /locus_tag="PK144_14535"
     CDS             complement(1486..3156)
                     /locus_tag="PK144_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNF66990.1"
                     /translation="MTTPLARSGARILVDQLLALGADLGFCVPGESYLPVLDALYDAR
                     DHFRLITCRNEGGAAFMAEAAGKLTGRPGLCFVTRGPGACNAAIGVHTAQQDSTPMLL
                     FIGQVGRAFRGREAFQEVDYRQMYAPLAKWVTELDSAARIPEILGRAWHVATSGRPGP
                     VVIALPEDVLAETASVADAVPLPLAAPPLAESELAALCERLAAAERPMLIVGGSNWSA
                     AGRAALHAFAEGWQLPVAAGFRRQDIFDNRRAQYAGELGTSVAPTLARRVREADLLLV
                     LGSRLGEMTTNGYTLVEAPLPRQTLIHVHPDALELNRVYRPALALATHPAQAAVQLAA
                     RPAPAAIRWADWTTAARAEHEANRQPGPMAGALDLGEVMRVLDAALPADAIVCNGAGN
                     YTGWPQRFHCFSHYPSQLAPTSGAMGYGVPAAIAAAAVHPTRIAVAFAGDGCFQMNGQ
                     ELASAVQAGLKPLILVIDNGQYGTIRMHQERHFPDRRIATALCNPDFAALARACGAFG
                     ERIECTGAFAPALERALDCDRAALLHLIVDPEQISTRQTLTALRAATR"
     gene            complement(3239..3892)
                     /locus_tag="PK144_14540"
     CDS             complement(3239..3892)
                     /locus_tag="PK144_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNF66991.1"
                     /translation="MPVVAETPTSPADGAPIRPANAPPAPLAERLRRLRTARGWTLAQ
                     AAKACAVARSTLSKIENAQMSPTYELLLKLADGFGLGIGELLAADDAGPAHGGGRRSL
                     ARPGSGDGSPEFEFGGCAHRPLCADLAGKRMQPYLSRVLDPGAAPAGWSRHDGEEFLY
                     VLAGRVQVLTEFYAPAELGPGEGCYLDSRMGHRVERLGDEEPRLLWIATRPQAGDAP"
     gene            4164..5345
                     /locus_tag="PK144_14545"
     CDS             4164..5345
                     /locus_tag="PK144_14545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF66992.1"
                     /translation="MRERYDVVIVGGAAVGSAAAYFLAADPAFAGASLLVVEKDASYR
                     EAATTLSAASIRHQFSTPENIRLSQFGTAFLREVGTALRVDDEVPNVGFREGGYLFLA
                     GPEGLTALETRHETQRALGADVRLLKPQLLAECFPWLATGDLAGASLGLSGEGWLDAW
                     SLLRALKRKARSLRAEYVEDCVVGLSRRGRRIESVRLAGGATVGCRWLLNCAGTGATS
                     LAALAGIELPVEARKRCVFHFQCATPISGSPLVVDPSGAWFRPEGPGWIAGIAPPPGA
                     DPVDPGFEVDWSQWEERLWPLLAARVPAFEAVRVKSAWAGHYDVNTLDCNAILGPHPG
                     LDNLLFANGFSGHGLQQAPAVGRALAEWVTTGASRSLDLSRFGWARVLAGQALRESEV
                     V"
BASE COUNT          740 a         1883 c         2071 g          749 t
ORIGIN      
        1 attgagggtg ttggtcgcgc ggcgcatgta ggacttccag gcatcggagc cggattcgcg
       61 cccgccgccg gtttccttgt cgccgccgaa ggcaccgccg atctcggcgc ccgaggtgcc
      121 gaggttgacg ttggcgatgc cgcagtccga gccggtcgcc ccgaggaact gcccggcctc
      181 gcgcagatcg ttcgtgaaga tcgccgagga cagcccctgc ggcacgtcgt tgttgagcgc
      241 gatcgcctcg tcgagcgtgc ggtagcgcag cacgtacagg atcggcgcga aggtctcgcg
      301 gcgcacggct gcgctctgcg ccggcatcgt cagcagcgcc ggcgtcacgt agaaggcttc
      361 gggcatgcgc accttcagca cgcgctcgcc gccgcagacc agccggccgc cttcggcgac
      421 ggccgcggcg agggcgctct gcatggccgt gaacgcggct tcgtcgatca gcggcccgac
      481 cagcgtgccg gcctgacgcg gatcgccgac gcgggcgctg acgaaggccg cctgcaggcg
      541 ttcgagcagc gcatcgtgga tcgacgtgtg cacgatcagc cggcgcgtgc tggtgcagcg
      601 ctggccggcg gtgccgatgg cgccgaagac gatgccgcgc agcgccagtt cgagatcggc
      661 cgagggcgcg acgatcacgg cgttgttgcc gccgagttcg agcagcgtgc gcccgaagcg
      721 tgccgcgacg cgcgggccga gtacccggcc catcgccgtc gagccggtcg ccgacaccag
      781 cgcgacgcgc gcatcgtcgg cgagctgctc accggccgcg cgcgcgccga tcagcagctc
      841 cgacaggccc tcgggcgcct cgccccagcg cagcagcgcg cgccggaaca gcgcctggca
      901 ggcaagcgcg ctcagcggcg tcttctcgga cggcttccag accaccggat tgccgcagac
      961 cagcgccagc gcgaagttcc aggcccagac cgcgaccggg aagttgaagg cgctgatgac
     1021 gccggtgacg ccgagcggat gccaggtttc catcatccgg tgaccgggac gctcggaggc
     1081 gatggtcagg ccgtgcagct gccgcgacag gccgacggcg aagtcgcaga tgtcgatcat
     1141 ctcctgcact tcgccctcgc cctcggcggc gatcttgccg gtttcgagcg tcaccagcgc
     1201 gccgagcgcg gccttgttgg cgcgcagctc ctcgccgagc agccgcacca gctcgccgcg
     1261 ccgcggcgcc ggcaccgtgc gccatctgtg aaaggccaga tgcgcccggg cgatcgccgc
     1321 gtcgatgtcg gcgggcgtgt ggctgcgcac gcgcgcgagc acggtgccgt cgatcggcga
     1381 acgggcgatc aggtcaccgt cgcgcagcgc atcggggctg aggccgaggg agcggagcag
     1441 ggggtcggac atgggcaagg actcgatgcg gaaaggggag aacgctcagc gcgtggccgc
     1501 gcgcagcgcc gtcagcgtct ggcgcgtgct gatctgttcg ggatcgacga tgaggtgcag
     1561 cagggccgcg cgatcgcagt cgagcgcgcg ttcgagggcc ggcgcgaagg cgccggtgca
     1621 ttcgatgcgc tcgccgaagg cgccgcaggc gcgggcgagg gcagcgaagt cggggttgca
     1681 gagcgccgtg gcgatgcgcc ggtccgggaa gtggcgttcc tggtgcatgc ggatggtgcc
     1741 gtactggccg ttgtcgatga cgaggatgag cggcttcaaa ccggcctgca ccgcgctcgc
     1801 cagttcctgg ccgttcatct ggaagcagcc gtcgccggcg aaggcgacgg cgatgcgcgt
     1861 cggatgcacg gccgccgccg cgatcgcggc cggcacgccg tagcccatcg cgccgctggt
     1921 cggggcgagc tgcgacggat agtgcgagaa gcagtgaaag cgctgcggcc agccggtgta
     1981 gttgccggcg ccgttgcaga cgatggcatc ggccggcagc gcggcatcga gcacgcgcat
     2041 gacctcgccg agatcgagtg ccccggccat cgggccgggc tgacggttgg cctcgtgctc
     2101 ggcgcgcgcc gcggtggtcc agtccgccca gcggatggcg gccggtgccg ggcgcgcggc
     2161 gagctgcacg gcggcctgcg ccgggtgcgt cgcgagcgcg agcgccgggc ggtagacgcg
     2221 gttcagctcc agcgcatcgg ggtggacgtg gatcagcgtc tggcgcggca gcggcgcttc
     2281 gaccagcgtg tagccgttcg tcgtcatctc gccgaggcgc gagccgagca ccagcagcag
     2341 gtcggcctcg cgcacgcgcc gcgccagcgt cggcgcgacc gaggtgccga gctcgccggc
     2401 gtactgcgcg cggcggttgt cgaagatgtc ctgccggcgg aagccggcgg cgaccggcag
     2461 ctgccagcct tcggcgaagg catgcagcgc cgcgcgcccg gcggcgctcc agttgctgcc
     2521 gccgacgatc agcatcggcc gctcggcggc ggcgagccgt tcgcagagcg cggccagctc
     2581 gctttcggcg agcggcggcg cggcgagcgg cagcggcacg gcatcggcga cgctcgcggt
     2641 ttcggcgagc acgtcctcgg gcagcgcgat caccaccggt ccgggccggc cgctggtggc
     2701 gacgtgccag gcgcggccga ggatttccgg aatgcgcgcg gcgctgtcga gttcggtcac
     2761 ccacttcgcg agcggcgcgt acatctgccg gtagtcgacc tcctggaagg cttcgcggcc
     2821 gcggaaggcg cgaccgacct gaccgatgaa cagcagcatc ggcgtcgaat cctgctgcgc
     2881 cgtgtgcacg ccgatcgcgg cattgcaggc accgggcccg cgcgtgacga agcagagccc
     2941 ggggcggccg gtcagcttgc cggcggcctc ggccatgaac gcggcgccgc cttcgttgcg
     3001 gcaggtgatc agccggaagt ggtcgcgggc gtcgtacagc gcatcgagca ccggcagata
     3061 gctctcgccg ggcacgcaga aaccgagatc ggcgccgagc gcgagcagct gatcgacgag
     3121 gatgcgcgca ccgctacggg caaggggagt ggtcacgatc tgggctccgg tgcgtccggg
     3181 aaggacaggg aatgagggtt gtttccggca cgggccatcg accggcggcg tccgggaatc
     3241 acggcgcgtc gccggcctgc gggcgcgtgg cgatccacag caggcgcggt tcctcgtcgc
     3301 cgaggcgctc gacgcggtgg cccatgcggc tgtcgagata gcagccctcg ccgggaccca
     3361 gctcggccgg ggcgtagaac tcggtcagca cttgcacgcg cccggcgagc acgtacagga
     3421 attcctcgcc gtcgtggcgg ctccagccgg ccggtgccgc gccggggtcc agcacgcggc
     3481 tcaggtaggg ctgcatgcgc ttgccggcga gatcggcgca cagcggccgg tgcgcgcagc
     3541 cgccgaattc gaattccgga ctgccgtccc cgctcccggg ccgcgcgagg ctgcgccggc
     3601 cgccgccgtg cgccggtccg gcgtcgtcgg cggcgagcag ttcgccgatg ccgagtccga
     3661 agccgtcggc gagcttcagc agcagctcgt aggtcggcga catctgcgcg ttctcgatct
     3721 tcgagagcgt cgagcgcgcc accgcgcagg ctttggcggc ctgcgccagc gtccagccgc
     3781 gcgccgtgcg caggcggcgc aggcgctcgg cgagcggcgc cggcggcgcg ttcgcgggcc
     3841 ggatcggggc gccatcggca ggggaggtcg gggtttcggc gacgacgggc atggggtgtg
     3901 ctccgcaggc agccgtccgg acttgccgga gcggacaaaa gtgattccaa tgtagcacgg
     3961 cgtttccgat cggaaacggc gatccccgtc cggctgtcat acctctttcg gagaactgac
     4021 gggatcggtc cgctgcgcat actgcgccgc cgcccagtcg ccgcgcgacc gtgcgcgcgc
     4081 gggtacggga accgtgccgg cggagcttcg ccggcagccg atggaagcaa acgaggccga
     4141 ggagagcagc agacgtgcag cccctgcgtg agcggtacga tgtggtgatc gtcggcggtg
     4201 ccgcggtcgg cagtgcggca gcctatttcc tggctgccga tccggccttt gccggggcct
     4261 cgctgctcgt ggtcgagaag gacgcgagct accgcgaggc ggcgacgacg ctgtcggcag
     4321 cctcgatccg ccaccagttc tcgacgcccg agaacatccg gctgtcgcag ttcggcaccg
     4381 ccttcctgcg cgaggtcggc acggcgctgc gcgtcgatga cgaggtgccg aacgtcggtt
     4441 tccgcgaggg tggctatctg ttcctcgccg gacccgaagg gctgacggcg ctcgaaaccc
     4501 ggcacgagac gcagcgcgcg ctcggcgccg atgtgcgcct gctcaagccg caactgctcg
     4561 ccgaatgctt tccgtggctc gcgaccggcg atcttgccgg tgcctcgctc ggtctgtccg
     4621 gcgaaggctg gctcgatgcc tggagcctgc tgcgggcact gaagcgcaag gcgcgctcgc
     4681 tgcgcgccga gtacgtcgag gactgcgtgg tcggcctgag ccggcgcggc cggcgcatcg
     4741 aatcggtgcg cctggccggc ggcgcgacgg tcggctgccg ctggctgctc aactgcgccg
     4801 gcaccggcgc caccagcctc gccgccctcg ccggcatcga gctgccggtc gaggcgcgca
     4861 agcgctgcgt gttccatttc cagtgcgcga cgccgatcag cggcagtccg ctggtggtcg
     4921 acccgtccgg cgcctggttc cggcccgaag gtccgggctg gatcgccggc atcgccccgc
     4981 cgcccggggc cgatccggtc gatccgggct tcgaggtcga ctggagccag tgggaggagc
     5041 gcctgtggcc gctgctcgcc gcgcgcgtgc cggccttcga ggcggtgcgc gtcaagagcg
     5101 cctgggccgg gcattacgac gtcaacacgc tcgactgcaa cgccatcctc ggcccgcacc
     5161 cgggcctcga caacctgctg ttcgccaacg gcttcagcgg ccacggtctg cagcaggcgc
     5221 cggcagtcgg ccgcgcactc gccgaatggg tcacgaccgg cgcctcgcgc agcctcgacc
     5281 tgtcgcgttt cggctgggca agggtgctgg ccgggcaggc actgcgcgag agcgaggtgg
     5341 tgtgagggcg gggcaggccg gtcgcccggg tcctgcacgc aggaaagaca aaggccggcc
     5401 gtcttgcgac gaccggcccg aatacatcct ggcccttccg tgg
//
LOCUS       DAOQIE010000266         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8527_length_5406_cov_7.707718, whole genome shotgun sequence.
ACCESSION   DAOQIE010000266 DAOQIE010000000
VERSION     DAOQIE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_5406_cov_7.707718"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="urtE"
                     /locus_tag="PK144_14550"
     CDS             complement(<1..674)
                     /gene="urtE"
                     /locus_tag="PK144_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HNF66993.1"
                     /translation="MLTVDQVNLRYGQSQILWNMNLTVRRGSCTCLMGRNGVGKTTTL
                     RAIMGLAPTFGGQVRFNGANLLKQPAHQRARIGIGYVPQGREIFPQLTVRENLQVGLG
                     IRPNGLEAVPERIFELFPILKQFLHRRGGDLSGGQQQQLAIGRALVLEPELLILDEPN
                     EGIQPNIVREIGDIIIRLNREIGLTVLLVEQKLPFARRLGTDFAIMEKGRTVATGTMP
                     ELTDEVV"
     gene            complement(713..1612)
                     /gene="urtD"
                     /locus_tag="PK144_14555"
     CDS             complement(713..1612)
                     /gene="urtD"
                     /locus_tag="PK144_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNF66994.1"
                     /translation="MNPVIPPVTPAGDALKGAQMLHDASEAPPKPPAAPREPLPADVR
                     DRPVPDVSHGAILYLESVSVSFDGFKAINDLNLSIDDGELRCIIGPNGAGKTTMMDII
                     TGKTRPDTGTVYFGQNVNLLELRESEIAQLGIGRKFQKPTVFQGHSVFENLELAMQAD
                     KRVRRALFARLSDEQRARIAGVLATIGLNGYERQLAGSLSHGQKQWLEIGMLLMQNPR
                     LLLVDEPVAGMTEQETEQTAELLNSLAGKHSVVVVEHDMAFVRSIARKVTVLHEGHVL
                     AEGTMDAIQSNPKVVEVYLGEGA"
     gene            complement(1609..2712)
                     /gene="urtC"
                     /locus_tag="PK144_14560"
     CDS             complement(1609..2712)
                     /gene="urtC"
                     /locus_tag="PK144_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNF66995.1"
                     /translation="MHGIRNLHTTRGWVGFFVVAALLLVAVPVLNALPETSPLHVPDW
                     LIPLAGKFCCYALVALAMDLIWGYTGILSLGHGVYFALGGYAMGMYLMRSMAGEGVYR
                     SELPDFMVFLNWKELPWFWHGFEYFPFALLMALAVPGLLAFVFGWFAFRSRIKGVYFS
                     IITQAMTFALMLLFFRNETGFGGNNGLTDFKRLLGFTLSEPSVRVALYWLTALVLLGV
                     YLLARAIVRSRAGRVLTAIRDAESRVMFCGYEPLYYKLAIWTLSAVLCGLAGALYVPQ
                     VGIINPSELAPAQSIEMAVWVAVGGRGTLVGAVIGAASVNGLKSVFTAVAPEFWLYAL
                     GGLFVLVTLFLPEGLTGLFRRLRRSRATEAART"
     gene            complement(2717..4333)
                     /gene="urtB"
                     /locus_tag="PK144_14565"
     CDS             complement(2717..4333)
                     /gene="urtB"
                     /locus_tag="PK144_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNF66996.1"
                     /translation="MNPPVRSRPSLSTLAALLFCLLLLPLAQPVSAAVDPKIDATLAA
                     LAEDPKTLRSALDVLAQSRDPRASRVLVALDEGRLTIAPGAVLIRTRDGQRVNAADGQ
                     PWTGAAGDAPAMTNAVRRVLASSIERARLRSEDRAERLGAARALNDRPSASAEPALRA
                     ALAEEQDPEVVTALKLALAPLDLDSPDKDKKLAALAVLANDGELTVLGRLQPLASGDD
                     PEIRAAAQKAITAIGDRQWLRDQGANLFYGLSLGSVLLLAALGLAITFGLMGVINMAH
                     GELLMIGAYATYVVQNLFRAYLPGAFDWYLLAALPVAFLSAAAVGWALERVVLRHLYG
                     RPLETLLATFGLSLILIQTVRLIFGAQNVEVANPSWLSGGIQLTQQLVLPYNRIATIL
                     FALAVLVTVWLLFQKTRLGLRVRAVTQNRGMAAAMGIDTRRVDAGTFALGAGVAGLAG
                     VALSQLGNVGPELGQAYIVDSFMVVVLGGVGKLAGAVAGGMGLGIVNKFLEPSMGAVL
                     GKIALLGFIILFIQWRPQGLFAMKGRAVEG"
     gene            complement(4612..>5406)
                     /locus_tag="PK144_14570"
     CDS             complement(4612..>5406)
                     /locus_tag="PK144_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HNF66997.1"
                     /translation="VLLGTDYVYPRTTNKILRAFLHSKGVSDDDIMETYTPFGHSDYQ
                     TIVADIKKFASAGKRTAVVSTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRG
                     IDTKPLVGHLAAWNYFMSIKSPENEAFIKAWKEYVAKNKLPGGDKRVTNDPMEATYTG
                     IHMWAQAVVQAGTTDVDAVRQALGYQTFKTPSGFVESLDAKNHHLHKPVYIAEIQADG
                     QFNIVWKTEGPIRAQAWSPYIPDSAKKVADWTYPWVCGNCTEPKYK"
BASE COUNT          865 a         1841 c         1887 g          813 t
ORIGIN      
        1 accacctcgt cggtcagctc cggcatcgtg ccggtggcga cggtgcggcc cttctccatg
       61 atcgcgaagt cggtgccgag gcggcgtgcg aacggcagct tctgttcgac cagcagcacg
      121 gtcagaccga tctcgcggtt cagccggatg atgatgtcgc cgatctcgcg cacgatgttc
      181 ggctggatgc cctcgttcgg ctcatcgagg atcagcagtt ccggttcgag caccagcgcc
      241 cggccgatcg cgagctgctg ctgctggccg ccggacagat cgccgccgcg ccggtgcagg
      301 aactgcttca ggatcgggaa cagctcgaag atgcgctcgg gcaccgcttc gagaccgttc
      361 gggcggatgc cgaggccgac ctgcaggttc tcgcgcacgg tgagctgcgg gaagatctcg
      421 cgcccctgcg gcacgtagcc gatgccgatg cgcgcgcgct ggtgcgcggg ctgcttcagc
      481 aggttcgcgc cgttgaaccg cacctgcccg ccgaaggtcg gcgccagccc catgatcgcg
      541 cgcagcgtcg tggtcttgcc gacgccgttg cggcccatca ggcaggtgca cgagccgcgg
      601 cgcaccgtca ggttcatgtt ccagaggatc tggctctgcc cgtagcgcag gttgacctga
      661 tcgaccgtca gcagcgcatc ggccgccgtg gattcctgca gcaccgccgc catcacgccc
      721 cctccccgag atacacctcg accaccttcg gattgctctg gatcgcgtcc atcgtgccct
      781 cggcgagcac gtggccctcg tgcagcaccg tgaccttgcg cgcgatcgaa cgcacgaagg
      841 ccatgtcgtg ctcgacgacg acgaccgaat gctttccggc cagcgagttc agcagctcgg
      901 cggtctgctc ggtctcctgc tcggtcatgc cggcgaccgg ctcgtcgacc agcagcaggc
      961 gcgggttctg catcagcagc atgccgatct ccagccactg cttctggccg tgcgacaggc
     1021 tgccggcgag ctggcgctcg tagccgttga ggccgatggt cgcgagcacc ccggcgatgc
     1081 gtgcgcgctg ctcgtcgctc aggcgcgcga acagcgcccg gcgcacgcgc ttgtcggcct
     1141 gcatcgccag ttcgaggttc tcgaacaccg agtggccctg gaacacggtc ggcttctgga
     1201 acttgcggcc gatgccgagc tgcgcgatct cggactcgcg cagttcgagc aggttgacgt
     1261 tctggccgaa gtacacggtg ccggtgtccg gacgggtctt gccggtgatg atgtccatca
     1321 tcgtcgtctt gccggcgccg ttcgggccga tgatgcagcg cagctcgccg tcgtcgatcg
     1381 acaggttcag gtcgttgatc gccttgaagc cgtcgaagct gacgctgacg ctttcgaggt
     1441 acaggatcgc gccgtgcgaa acgtccggca ccggccggtc gcgcacgtcg gccggcagcg
     1501 gctcgcgtgg cgcagccggc ggtttcggcg gcgcttcgga ggcgtcgtgc agcatctgcg
     1561 cgcccttcag ggcatcaccg gcaggggtga cgggcgggat cacgggattc atgtgcgggc
     1621 agcctctgtg gctcgggagc ggcgcaggcg ccggaacagg ccggtcaggc cctcgggcag
     1681 gaacagcgtc accagcacga acaggccgcc gagcgcgtac agccagaact ccggcgccac
     1741 ggccgtgaac acgctcttga ggccgttgac gctggccgcg ccgatcacgg ccccgaccag
     1801 cgtgccgcgc ccgccgaccg cgacccagac agccatctcg atcgactgcg ccggcgccag
     1861 ctcgctcggg ttgatgatgc cgacctgcgg cacgtacagc gcaccggcca gaccgcagag
     1921 caccgccgac agcgtccaga tcgcgagctt gtagtacagc ggctcgtagc cgcagaacat
     1981 cacgcggctc tcggcgtcgc ggatcgcggt cagcacgcgc ccggcacgcg agcgcacgat
     2041 cgcgcgggcg agcagataca cgccgagcag caccagcgcc gtcagccagt acagtgccac
     2101 gcgcaccgag ggctcactga gcgtgaagcc gagcaggcgc ttgaagtcgg tcaggccgtt
     2161 gttgccgccg aagccggtct cgttgcggaa gaacagcagc atcagcgcga aggtcatcgc
     2221 ctgcgtgatg atcgagaagt acacgccctt gatgcgcgag cggaacgcga accagccgaa
     2281 cacgaaggcg agcagccccg gcacggcgag cgccatcagc agcgcgaacg ggaagtactc
     2341 gaagccgtgc cagaaccacg gcagctcctt ccagttcagg aacaccatga agtccggcag
     2401 ctccgagcgg tagacgccct cgccggccat gctgcgcatc aggtacatgc ccatcgcgta
     2461 gccgccgagc gcgaagtaca cgccgtggcc gagcgacagg atgccggtgt agccccagat
     2521 caggtccatc gccagcgcca ccagcgcgta gcagcagaac ttgccggcga gcgggatcag
     2581 ccagtcgggc acgtgcagcg gactggtctc gggcagtgcg ttgagcaccg gcaccgccac
     2641 cagcagcagt gccgcgacca cgaagaagcc gacccagccg cgggtcgtat gcagattgcg
     2701 gatgccgtgc atcggttcag ccctccaccg cccggccctt catcgcgaac agcccctgcg
     2761 gccgccactg gatgaacagg atgatgaagc cgagcagcgc gatcttgccg agcaccgcgc
     2821 ccatcgacgg ctccaggaac ttgttgacga tgccgagccc catgccgccg gcgaccgcgc
     2881 cggcgagctt gccgacgccg ccgagcacca cgaccatgaa ggaatcgacg atgtaggcct
     2941 gcccgagctc cgggccgacg ttgccgagct gcgacagcgc cacgccggcg agccccgcca
     3001 cgccggcgcc gagcgcgaag gtgccggcat cgacgcggcg cgtgtcgatg cccatcgccg
     3061 cggccatgcc gcggttctgc gtcaccgcgc gcacgcgcag gccgagccgg gtcttctgga
     3121 acagcagcca gaccgtcacc agcaccgcca gcgcgaacag gatcgtcgcg atgcggttgt
     3181 acggcagcac cagctgctgc gtgagctgga tgccgcccga cagccacgag ggattggcga
     3241 cctcgacgtt ctgcgcaccg aagatcaggc gcacggtctg gatcaggatc agcgacaggc
     3301 cgaaggtcgc gagcagggtt tcgagcggcc ggccgtacag gtggcgcagc acgacgcgct
     3361 ccagcgccca gccgaccgcc gccgcgctca ggaaggccac cggcagcgcc gcgagcagat
     3421 accagtcgaa ggccccgggc aggtaggcgc ggaacaggtt ctgcacgacg taggtcgcgt
     3481 aggcgccgat catcagcagc tcgccgtgcg ccatgttgat gacgcccatc agcccgaagg
     3541 tgatcgccag cccgagcgcg gcgagcagca gcacgctgcc gagcgacagg ccgtagaaca
     3601 ggttggcgcc ctggtcgcgc agccactggc ggtcaccgat ggccgtgatc gccttctgcg
     3661 ccgcagcgcg gatttcgggg tcatcgccgc tcgcgagcgg ctgcagccgg ccgagcacgg
     3721 tcagctcgcc gtcattggcg agcacggcga gtgcggcgag cttcttgtcc ttgtcgggac
     3781 tgtcgagatc gagcggcgcc agcgcgagct tcagcgccgt caccacttcc gggtcctgct
     3841 cctcggcgag tgcggcgcgc agcgccggtt cggcgctcgc cgatggccgg tcgttcaatg
     3901 cccgcgcggc accgaggcgc tcggcgcggt cctcggagcg caggcgcgcg cgctcgatgg
     3961 aactggcgag cacgcggcgc acggcgttgg tcatcgccgg cgcatcaccg gcggcaccgg
     4021 tccacggctg accgtcagcg gcgttgaccc gctggccgtc gcgggtgcgg atgaggacgg
     4081 cgcccggcgc gatcgtcagc cgaccctcgt cgagggcgac cagcacgcgg ctggcgcgcg
     4141 gatcacggct ctgtgccagc acgtcgaggg cgctgcgcag cgtcttcggg tcttcggcca
     4201 gcgccgcgag cgtggcatcg atcttcggat cgactgcggc ggaaacgggc tgtgccagcg
     4261 gcagcagcag gaggcagaac agcagtgccg ccagagtcga caggctcgga cgcgaccgga
     4321 caggaggatt catgcgcaac ctcggggggc cgacggaaag aagccgccgg gacgccggtc
     4381 tgcgggccga agcctgggtt cttcggacgg gtggccggcg tccccgcggg gcgacgctgc
     4441 cggagccggc gaaagaaccg gctccggctc gccgtcgacc gcaggcagga ctgcggggag
     4501 cggcggacga tgcgaaagcg tgcggcccga ccctgcgggt gacgcctgcg catcgcatcc
     4561 gtgccgcgga cgcggcacgg aacgcgcagc ggactggcgc ggcgggacgg cttacttgta
     4621 cttcggttcg gtgcagttgc cgcagaccca cggataggtc cagtcggcga ccttcttggc
     4681 gctgtccggg atgtacggcg accaggcctg ggcgcggatc gggccttcgg tcttccagac
     4741 gatgttgaac tggccgtcag cctggatctc ggcgatgtac accggcttgt gcaggtggtg
     4801 gttcttggcg tcgagcgact cgacgaagcc cgacggcgtc ttgaaggtct ggtagccgag
     4861 cgcctggcgc acggcatcga cgtcggtggt gccggcctgc acgaccgcct gcgcccacat
     4921 gtggatgccg gtgtaggtgg cctccatcgg gtcgttggtc acgcgcttgt cgccgcccgg
     4981 cagcttgttc ttggcgacgt attccttcca cgccttgatg aaggcctcgt tctccggcga
     5041 cttgatcgac atgaagtaat tccaggccgc gaggtgaccg accagcggct tggtgtcgat
     5101 gccgcgcagt tcctcctcac cgaccgagaa cgccacgacc ggcacgtcgg tggccttcag
     5161 gccggcgttg ccgagttcct tgtagaacgg cacgttggaa tcgccgttga tcgtcgagac
     5221 cacggccgtg cgcttgccgg cggaggcgaa cttcttgatg tcggcgacga tggtctggta
     5281 gtcgctgtgc ccgaacggcg tgtaggtctc catgatgtcg tcatcggaga cgcccttcga
     5341 atgcaggaag gcgcgcagga tcttgttggt ggtgcgcggg tacacgtagt cggtgccgag
     5401 cagcac
//
LOCUS       DAOQIE010000267         5379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8598_length_5379_cov_6.926935, whole genome shotgun sequence.
ACCESSION   DAOQIE010000267 DAOQIE010000000
VERSION     DAOQIE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8598_length_5379_cov_6.926935"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1574)
                     /locus_tag="PK144_14575"
     CDS             complement(291..1574)
                     /locus_tag="PK144_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF66998.1"
                     /translation="MSARRLAPVAAAALWSASPFALADFSDWPASVELSFERDPAHAD
                     TRLNPDGRIVDTPDPSTRAALRLQPKLDGGDVSLRADLWAEWREEDSISDGGRVFAQE
                     AMLDLRFSPAVTVSGGVAPFRWGTAYVWNPSNPFPDEQRNFTDRARTWRAPGTPQLGL
                     EWTGSADTIGVYGVSYRPTTALYGRPIDLDREPAVAVRWRHQFEAADLTLTFATPERE
                     PFFGAALSATVSDQLELHAEAGVRGKRQTLLPVARDIPGPGGTVPLYTLERDDDAGRS
                     LQWLVGGQYTTEGLTNVIVEFYRNGEGYSDREYDHLLDYADDATARLDDPLFGSAYAG
                     YLAGANALTGMMRRNYGFVRLSRDRAIGDFDLRGYWRYGFDDGSSLGGVALIHPLGDS
                     ARVRANLEVYGGRADSETRLIPSRGSAVIALEMLF"
     gene            complement(1571..2359)
                     /locus_tag="PK144_14580"
     CDS             complement(1571..2359)
                     /locus_tag="PK144_14580"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNF66999.1"
                     /translation="MLPRIRSLHPLRVARTALLALLFAAALPATAPAAPDAVALLERA
                     DGYRNFRGKPFAFDLGLTSIEPGESERSFRLRAEILDPHTSLVSYIGPATERGKALLM
                     NGSNLWFYSPSSSKPIRITPQQRLLGEASNGDVASTDFSGDYTPVWLASESVDGVACQ
                     KLELTAKPGTLATYQKLHLWLRSDDARPVKADFFAPSGKLLKTAYYRRYEPVAAAGGK
                     PQLTEIEIVSAAGEDKRTLMRYENFSLAPLAPERFSLGNLGKSR"
     assembly_gap    2550..2649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2650..3879)
                     /locus_tag="PK144_14585"
     CDS             complement(2650..3879)
                     /locus_tag="PK144_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF67000.1"
                     /translation="MPFLLKVALRNIRRHRRRSLVTLATVASAVIGIVLFGGFIEANY
                     TGLRESVIRSQYGHAQIHVAGYEAGHRADPDKVRLSPESAEAVRTLLEKDRHVLMSSR
                     RIEFAALLGNEKTSQAVIVRGVDPDTEALINSALTIIAGDELAADDADGVLLGEGLAQ
                     ALNVKPGATLTMLGSTVDGTMNAVDVRVAGIFRSFATEYDDRAVMMGLTRAGELLGTP
                     SVDTVVVLLDDTAALDGVMERFGAAAKDAGLKLEWQTWAQLASYYHKVVALYDGFFLF
                     IALVIVVVVMFGITNTMLVAVMERTAEIGTLRALGTRRGGIVRQFLTEGVVLGTGSAL
                     IGVVLGIVAAHGITALEIMMPAPPGSSKGFPLRIEDVPGLWIGCVLAVAGIAAFATLL
                     PAVRAARQPVVTSLRHV"
     gene            complement(4033..4764)
                     /locus_tag="PK144_14590"
     CDS             complement(4033..4764)
                     /locus_tag="PK144_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF67001.1"
                     /translation="MSFLTTENVHKRYALGHVTVDALRGVDLVIGRGERVFLGGPSGS
                     GKSTLLHLLGGLDAPSSGRVTLDGQDLAAIDDARLSDFRARHIGFVFQNFNLMPVLTA
                     VENVEYPLQLLGERRRTAAARAMLEAVGLGAQADQYPNELSGGQQQRVAIARALVHRP
                     ALLIADEPTAALDSTTGAQVLELMLALSRDTGTTLVVCTHDRELLARAERRVLMRDGR
                     IEHDSGALPAQAANHAAAAALPESA"
     gene            complement(4884..>5379)
                     /locus_tag="PK144_14595"
     CDS             complement(4884..>5379)
                     /locus_tag="PK144_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF67002.1"
                     /translation="ALDGLDALVCVADPLPAAATADAIVAAALVATQRLLGSAKAATG
                     HWTRARTPGSIVVVCDIAGLCGREGLAASAAASGALLGMARSLAKELGRRQIAVNAVC
                     HGFVAGLDTGSALNGAEQRLFGAMGLGKPVTAAMLAADVLFLAGGGHGLTGQVLHADD
                     GLVI"
BASE COUNT          734 a         1771 c         2011 g          763 t
ORIGIN      
        1 acccctctcc cgcgagggga gaggggcttg aaaatcacgt tcttgcagcg tcgtcggttc
       61 ggtgcgggta ctggccggcg cggtgccggt ggttccccct ctccccaacc cctctcccgc
      121 gaggggagag gggcttgaaa atcatgttct tgcagcgatg ttcggggcgt tcaggcgtct
      181 ttcgtcgtgt cgtcgtggcg tcattcgtgg ccggcgcggg cgtgcgtcgg gccggcccgc
      241 gtcctgaaac ggacgcgggc ggtggattcc ggggatgcgc ggcgagccgc ctagaacagc
      301 atttcgagtg cgatgacggc gctgccgcgg ctcgggatca gccgggtctc ggagtcggcg
      361 cggccgccgt agacctcgag gttggcgcgc acgcgggcgc tgtcgccgag cgggtggatc
      421 agcgcgacgc cgccgaggct gctgccgtcg tcgaagccgt agcgccagta gccgcgcagg
      481 tcgaagtcgc cgatggcgcg gtcgcggctg aggcgcacga agccgtagtt gcgccgcatc
      541 atgccggtca gcgcgttggc cccggccaga tagccggcgt aggcgctgcc gaacagcggg
      601 tcatcgagcc gggcggtcgc atcgtcggcg taatcgagca ggtggtcgta ctcacgatcc
      661 gagtagccct cgccgtttcg gtagaactcg acgatgacgt tggtcaggcc ctcggtcgtg
      721 tactggccgc cgaccagcca ctgcaggctg cgcccggcat cgtcgtcgcg ctccagcgtg
      781 tagagcggca ccgtgccgcc ggggccgggg atgtcgcgcg ccaccggcag cagcgtctgg
      841 cgcttgccgc gcacgccggc ctcggcgtgc agttcgagct gatccgagac ggtcgccgac
      901 agcgccgcgc cgaagaacgg ttcgcgttcg ggggtcgcga aggtcagcgt caggtcggcg
      961 gcctcgaact ggtggcgcca gcgcacggcg accgccggct cgcgatcgag atcgatcggg
     1021 cggccgtaca gcgccgtcgt cggccggtag ctcacgccgt agacgccgat cgtgtccgcg
     1081 ctgccggtcc attccaggcc gagctgcggc gtgcccggcg cgcgccaggt gcgagcgcgg
     1141 tcggtgaagt tgcgctgctc gtccgggaac gggttcgacg ggttccagac gtaggccgtg
     1201 ccccagcgaa acggcgcgac gccgcccgag accgtcacgg ccggcgagaa gcgcaggtcg
     1261 agcatcgcct cctgcgcgaa cacccggccg ccgtcgctga tggagtcctc ctcgcgccat
     1321 tcggcccaga ggtcggcacg cagcgagaca tcgccgccgt cgagcttcgg ctgcaggcgc
     1381 agcgcggcgc gcgtcgaggg gtcgggcgtg tcgacgatgc ggccgtcggg gttcagccgc
     1441 gtgtcggcgt gcgccgggtc acgttcgaag gacagctcga cgctggccgg ccagtcggag
     1501 aagtcggcga gcgcgaacgg gctcgcactc cagagcgcag cggccgccac gggcgcaagg
     1561 cggcgcgcgc tcaccgggac ttaccgaggt tgccgaggct gaagcgctcg ggcgcgagcg
     1621 gggccagcga gaagttctcg tagcgcatca gcgtgcgctt gtcctcgccg gcggcgctga
     1681 cgatctcgat ctcggtcagc tgcggcttgc cgccggcggc ggcgaccggc tcgtagcggc
     1741 ggtagtacgc ggtcttcagc agcttgccgg acggcgcgaa gaagtcggcc ttgaccgggc
     1801 gggcgtcgtc gctgcgcagc cacaggtgca gcttctggta ggtcgcgagc gtgccgggtt
     1861 tggcggtcag ttcgagcttc tggcaggcga cgccgtcgac ggactcgctg gccagccaca
     1921 ccggcgtgta gtcgccggaa aagtccgtgc tcgcgacatc gccgttcgag gcctcgccga
     1981 gcaggcgctg ctgcggcgtg atgcggatcg gcttgctgct cgacggcgag tagaaccaca
     2041 gattgctgcc gttcatcagc agcgccttgc cgcgctcggt ggccgggccg atgtagctga
     2101 ccagcgaggt gtgcgggtcg aggatctcgg cgcgcagcct gaatgaacgc tcggactcgc
     2161 cgggctcgat gctggtcagg ccgaggtcga acgcgaacgg cttgccgcgg aagttgcggt
     2221 agccgtcggc gcgttcgagc agcgccacgg cgtcgggcgc ggccggggcg gtggccggca
     2281 gcgcggcggc gaacagcagg gcgagcagcg cagtgcgcgc gacgcgcagc gggtgcaggg
     2341 aacggattcg gggcagcatg gtgagagtcc tccgggggta tcgggcgggg gtcagatcag
     2401 tcacacgaac cagtgttgtt catccccctc tccccaaccc ctcccccgcg agggggaggg
     2461 gctgaaaaga cggcgtgttc cgttgtgatc gggatcagac gtggcgcagc gaggtgacga
     2521 ccggctggcg ggcggcacgc acggccggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnt cagacgtggc gcagcgaggt gacgaccggc tggcgggcgg cacgcacggc
     2701 cggcagcagc gtcgcgaagg cggcgatgcc ggcgaccgcg agcacgcagc cgatccacag
     2761 gcccggcaca tcctcgatgc gcagcgggaa gcccttcgac gagccgggcg gggccggcat
     2821 catgatctcc agggcggtga tgccatgcgc ggcgacgatg ccgagcacga cgccgatcag
     2881 cgccgagccg gtaccgagca ccacgccttc ggtcaggaac tggcggacga tgccgccgcg
     2941 gcgcgtgccg agcgcgcgca gcgtgccgat ctcggcggtg cgctccatga ccgccaccag
     3001 catggtgttg gtgatgccga acatcacgac gacgacgatg accagcgcga tgaacaggaa
     3061 gaagccgtca tagagcgcga cgaccttgtg atagtaggaa gcgagctgcg cccaggtctg
     3121 ccattcgagc ttcaggcccg cgtccttcgc ggcggccccg aagcgctcca tgacgccatc
     3181 gagcgcggcc gtgtcatcga gcagcacgac gacggtatcg accgagggcg tgccgagcag
     3241 ctcgccggcg cgcgtcaggc ccatcatcac ggcgcggtcg tcgtactcgg tcgcgaagct
     3301 cctgaagatg ccggcgacgc gcacgtcgac ggcgttcatc gtgccgtcga cggtgctgcc
     3361 gagcatcgtc agcgtcgcgc ccggcttcac gttcagcgcc tgcgcgaggc cctcgccgag
     3421 cagcacgccg tcggcgtcgt ccgcggccag ctcgtcgccg gcgatgatgg tcagcgccga
     3481 gttgatcagc gcctcggtgt ccggatcgac gccgcgcacg atcaccgcct ggctggtctt
     3541 ctcgttgccg agcagcgccg cgaactcgat gcggcgcgag ctcatcagca cgtgcctgtc
     3601 cttttcgagc agcgtgcgca cggcctcggc gctttccggc gacagccgca ccttgtccgg
     3661 atcggcgcgg tgcccggcct cgtagccggc gacgtggatc tgcgcgtggc cgtactggct
     3721 gcggatcacc gattcgcgca ggcccgtgta gttggcctcg atgaagccgc cgaacagcac
     3781 gatgccgatg acggcggaag cgacggtcgc cagcgtcacc agcgagcggc ggcggtggcg
     3841 gcggatgttg cgcagtgcga ccttgagcag gaagggcatg gtcgtgacct cggggcggtc
     3901 ggattcggtt cgggcggaat caggcggagc cttgccggtc acgcctgact gcgttgcttg
     3961 tcccccgctc tccaacccct ccccgcgagg ggggaggggc tgaaaggacc gggcgttcgg
     4021 atgcgatcgc tatcaggcgg attcgggcag ggcggcggcc gcggcgtggt tcgcggcctg
     4081 cgccggcagc gcgccgctgt cgtgctcgat gcgcccgtcg cgcatcagca cgcggcgttc
     4141 ggcgcgcgcc agcagctcgc ggtcgtgcgt gcagacgacg agcgtcgtgc cggtgtcgcg
     4201 cgacagcgcc agcatcagct ccagcacctg cgcgccggtc gtgctgtcga gcgccgcggt
     4261 cggctcgtcg gcgatcagca gcgccgggcg gtgcacgagt gcgcgcgcga tcgcgacgcg
     4321 ctgctgctgg ccgccggaga gctcgttcgg atactggtcg gcctgcgcgc cgaggccgac
     4381 ggcttcgagc atcgcgcgcg ccgcggccgt gcgcctgcgc tcgccgagca gctgcagcgg
     4441 gtattcgacg ttctcgaccg cggtcagcac cggcatcagg ttgaagttct ggaacacgaa
     4501 gccgatgtgg cgggcgcgga agtcgctgag gcgggcatcg tcgatcgcgg ccagatcctg
     4561 accgtcgagc gtgacgcggc cgctgctcgg cgcatcgagt ccgccgagca ggtgcagcag
     4621 cgtcgacttg ccgctgcccg aggggccgcc gaggaacacg cgctcgccgc gcccgatcac
     4681 gagatcgacg ccgcgcagcg catcgaccgt gacgtggccg agggcatagc gcttgtggac
     4741 gttttcggtg gtgaggaagc tcatgtctgc ggtcgctccg ggcgcggggc gtgctgccga
     4801 tcgccgtcgt gtcagggatg gggccggtcg ccggggctcg cgggcgcgca gcggcgggtc
     4861 cggggaaaga tcaggacgct gcgtcagatc accaggccgt catcggcgtg cagcacctga
     4921 ccggtcaggc cgtggccgcc gccggcgagg aacagcacgt cggcggccag catcgctgcc
     4981 gtcaccggct tgccgaggcc catcgcgccg aacagccgct gctcggcgcc gttcagcgcg
     5041 ctgccggtgt cgaggccggc gacgaagccg tggcagacgg cgttgacggc gatctgccgg
     5101 cggccgagtt ccttggcgag gctgcgcgcc atgccgagca gcgcgcccga ggccgcggca
     5161 ctcgccgcca gtccctcgcg tccgcacagg ccggcgatgt cgcagaccac gacgatcgag
     5221 cccggcgtgc gcgcgcgcgt ccagtgcccg gtcgcggcct tggcgctgcc gagcaggcgc
     5281 tgcgtggcga cgagcgcggc ggcgacgatg gcatcggccg tggccgcagc cggcagcgga
     5341 tcggcgacgc agaccagcgc gtcgagtccg tcgagcgcg
//
LOCUS       DAOQIE010000268         5328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8742_length_5328_cov_6.513749, whole genome shotgun sequence.
ACCESSION   DAOQIE010000268 DAOQIE010000000
VERSION     DAOQIE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_5328_cov_6.513749"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..995)
                     /locus_tag="PK144_14600"
     CDS             complement(360..995)
                     /locus_tag="PK144_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNF67003.1"
                     /translation="MEHTGLFIGALVTVYLLPGPDMLLILQTAGTQGRRAALAVAAGL
                     ALARCIHVTLAGLGLAALLHNSPQTFEGLRLVGAAYLVWLGLHIARSPLPCAQDPARE
                     PPMPAHSLAAAALRGLLTNLTNPKALLFCSMLLPQFVSVDATDPAGPFFRLGAVLVGI
                     GLLFDGLYVFAGVVIGHWLRRSVLRRRMQQWGFGALLVGFGLRLALVGRMD"
     gene            1128..1619
                     /locus_tag="PK144_14605"
     CDS             1128..1619
                     /locus_tag="PK144_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF67004.1"
                     /translation="MPILKPDRLDRRILEALQRDARLSNVELAEAIGLSPSPCLRRVR
                     LLEEAGVISGYHAALDRNAVGLGLTVFVGVKVGPHHEEASTAFREAVVQLPEVVSCHL
                     VSGEADFLLQVVVPDLPAYERFLLGTLLKLPGVSDIRSNFAIATVKAQTALPLAHLGA
                     GGS"
     gene            1784..3364
                     /locus_tag="PK144_14610"
     CDS             1784..3364
                     /locus_tag="PK144_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF67005.1"
                     /translation="MHDIAIVGGGLCGLALLRLLSGSGLDVVLFEARARLGGRVLSVP
                     ARGGHPGGDLGPGWYWPDTQPLLNGLITELGLRTFAQHDQGISLYVGGPDQPPASLGE
                     RPVHDGARRIDGGMGRIIEGLAHALPPARLQTGCRLHRLRDHGDHVELGFTDTAFGRT
                     VRARHVVLALPPRLIDETLAFEPALPAELRAAMRDTPTWMAAQAKLLAACEQPVWRVF
                     GCSGNAFVRHAQAVVGEVFDACDAAGTRAALGGFVTPPAALRRQIDLPAAVIGEFAKL
                     FGPTLRCTDFALQDWAAESLTCSRRDREQAESVHPGDAPLLRLPYWGGRLLFGGSETA
                     ARHAGYLEGALLAAARLAGELGRLAMTGNAAQAAPAAEREATLERLRACLQAPIADWR
                     AGLLAEYRRQLHVVLSGPHGGAAITQRALLGTVGVLYAAALDALVVSEAQVQGVAIER
                     GRSALTPPLLACFEGCNDALIATALEFNRGSCALSNFPQEHQPSAETLHAIRLDLAAA
                     WREFALTLNARLLPRSVA"
     gene            3594..4037
                     /locus_tag="PK144_14615"
     CDS             3594..4037
                     /locus_tag="PK144_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF67006.1"
                     /translation="MPEVIGIDHLYISVSDPARAEDFYDRAFVTALGFRKNRFALDGE
                     PHVQYYNRHFGYVLRPARSPATHDPYAPGLHHFCLRVDSIEDVVAAAGRLRAAGIEAS
                     PATHHPQYAPDYWACFFSDPDGIRLEITNFRQERRERHAQGAVSD"
     gene            4196..>5328
                     /locus_tag="PK144_14620"
     CDS             4196..>5328
                     /locus_tag="PK144_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNF67007.1"
                     /translation="MTDNGDSPDRPPYRPPAFASTDARSDAGSSAFPWLLFLIVLLPL
                     GLLMLGVAAYVGHARIDAMIAVERANTGIAVGVVSGRLNDELRVPVRHLQALAGELPV
                     RSAITSAEPGAAGLDAAFLSLMERNPNYDQVRWLDAGGRERVRLQREGGQVQRVASVA
                     LQDKSDRPYFREAIRLEAGEMYVSPLDLNIEHGRIEEPYKPMLRVATPVDDAEGVRRG
                     LLIINVLAQPILDAAEGSLGSSGNPVMLLNADGYSLRSPEPAQDFGFMFGRSERFATR
                     HAGIWAQMTETPAGQVEDAEGLWTWRRVSLASTAGKTLAGTAPEFIALTQVPGARLVQ
                     QRREVWLAVGGITAALLLAHTLLSLALAFALAGRSRAETGGLST"
BASE COUNT          785 a         1840 c         1897 g          806 t
ORIGIN      
        1 cggctgtgag ggcgcgacac tttgcactac caggggagcg ttgcacagcc ctggcccatg
       61 tggtgagtcc ggaagaattt tccgaaaatc aggattggct gccgtgtatg tgcgcggaca
      121 ggctgatttc ggaagagcgg gcagggcgga atgggagatg cgtgcaggtg gtacgacggg
      181 cagcattcga tggcaggatg cccagtcgct ttgtgcccgc cgtgcgccgg atggcgccgg
      241 catcaaccgc tgtgaggcaa tgagcatctg gcgtgaagcc atcgggccgt tcatggcgac
      301 gacccggtat tgatcatcgc aagctgtcgt tctcccttgc ggccacggca ccggcccgct
      361 cagtccatcc gcccgaccag cgccagcctc agcccgaagc ccaccagcag cgccccgaat
      421 ccccactgct gcattcgccg gcgcagcacg ctgcgtcgca gccagtggcc gatgacaacg
      481 ccggcaaaga catacagccc atcgaacagc agaccgatgc cgaccagtac ggcaccgagc
      541 cggaagaacg ggccggccgg gtcggtggca tcgaccgata cgaactgcgg cagcagcatc
      601 gagcagaaca gcagggcctt gggattggtg agattggtca gcagcccgcg caatgcggca
      661 gcggccagac tgtgcgccgg catcggcggc tcgcgggccg ggtcctgtgc gcagggcagc
      721 ggactgcgtg cgatgtgcag gccgagccag accagatacg ccgcgccgac caggcgcagc
      781 ccctcgaagg tctgcggcga gttgtgcagc agggccgcca ggccgagtcc ggccagcgtc
      841 acatgaatgc agcgcgccag cgccagccct gcagccaccg ccagcgcagc ccggcggccc
      901 tgcgtaccgg cggtctgcag gatcagcagc atgtccggac cgggcagcag atagaccgtg
      961 accagcgcac cgatgaacag ccccgtgtgt tccatgaccg ggcctcccgt ctgtggaatc
     1021 gagcgggcag ggtagacgca tgaccggagc cggggattgc gtattcagcc atccatgcct
     1081 acggttctgg cagaacatgc cattgattga tctttaatct gttgatcatg ccaatcctga
     1141 aacccgaccg gctcgaccgg cgcattctcg aagccttgca gcgcgatgcg cgcctgagca
     1201 acgtcgaact tgccgaggcc atcggtctgt cgccgtcgcc ctgcctgcgg cgcgtgcgcc
     1261 tgctggaaga ggccggcgtc atcagcggct atcacgcggc gctggatcgc aacgcggtcg
     1321 ggctggggct gacggtgttc gtcggcgtca aggtcggtcc gcaccatgaa gaagcctcga
     1381 cggcctttcg cgaggccgtc gtgcaactgc ccgaggtcgt ctcctgccat ctggtttccg
     1441 gcgaggccga cttcctgctg caggtggtcg tgcccgatct gccggcctat gaacgcttcc
     1501 tgctcggcac gctgctgaag ctgcccggcg tcagcgacat ccgcagcaac ttcgcgatcg
     1561 ccacggtcaa ggcgcagacg gcgctgccgc tcgcgcatct gggggcgggc ggcagctgac
     1621 gcggcagcga tccgacaatt ccgacggttc ctgtcggatt ccctaccgcc ccgctcgggc
     1681 tgccatgacg tgcaagtgcc tgaaccggcg gttgcctgtg tcaatttgca tggaacttgc
     1741 tcctgtgccc cgtgcagatt cgggttcggg gaagggctgg gccatgcatg acattgcgat
     1801 cgtcggtggc gggctgtgcg ggctggcgct gctgcggctg ctcagcggca gcgggctcga
     1861 tgtggtgctg ttcgaggcgc gggcgcggct tggcgggcgg gtgctgtcgg tgccggcgcg
     1921 cggcgggcat ccgggcggcg atctcgggcc gggctggtac tggccggaca cgcagccgct
     1981 gctgaacggc ctgatcaccg aactggggct gcgcaccttc gcgcagcacg atcagggcat
     2041 ctccctgtac gtcggcgggc cggaccagcc gccggcgagc ctcggcgagc ggccggtgca
     2101 tgacggtgcg cggcgcatcg acggcggcat gggccgcatc atcgaggggc ttgcgcacgc
     2161 gctgccgccc gcccgcctgc agaccggctg ccggctgcac cggctgcgtg accacggcga
     2221 tcacgtcgaa ctcggtttca ccgacacggc cttcgggcgc accgtgcgcg cccggcacgt
     2281 cgtgctggcg ctgccaccgc ggctgatcga cgagacgctc gcgttcgagc cggccctgcc
     2341 ggcggagctg cgcgcggcga tgcgcgacac gccgacctgg atggcggcgc aggccaagct
     2401 gctcgccgcc tgcgagcagc cggtctggcg cgtgttcggc tgctccggca atgccttcgt
     2461 gcgccatgcg caggccgtgg tcggcgaggt gttcgatgcc tgcgatgccg ccggcacgcg
     2521 cgccgcgctc gggggcttcg tcacgccgcc ggcggcgctg cgccggcaga tcgacctgcc
     2581 ggccgcggtc atcggcgagt tcgcgaagct gttcgggccg acgctgcgct gcaccgactt
     2641 cgcgctgcag gactgggcgg cggagtcgct gacctgcagc cggcgcgatc gcgagcaggc
     2701 cgagtccgtg catccgggcg atgcgccgct gctgcgcctg ccgtactggg gcggacggct
     2761 gctgttcggg ggttcggaaa ccgcggcccg gcacgcgggc tatctcgaag gggcgctgct
     2821 cgccgcggcc cggctggcgg gcgaactcgg ccggctggcg atgaccggga acgcggcgca
     2881 agccgcgccg gcagcggagc gcgaggccac gctcgagcgg ctgcgcgcct gtctgcaggc
     2941 cccgatcgcc gactggcgcg ccggcctgct cgccgaatac cggcggcaac tgcacgtcgt
     3001 gctgtccggg ccgcacggcg gggcggcgat cacgcagcgg gcgctgctcg gcacggtcgg
     3061 tgtgctgtat gccgcggcgc tcgatgcgct ggtcgtcagc gaggcgcagg tgcagggcgt
     3121 ggcgatcgag cgcggacgct cggcgctgac accgccgctg ctcgcgtgct tcgagggctg
     3181 caacgatgcg ctgatcgcga ccgcgctcga attcaaccgc ggttcctgcg cgctctcgaa
     3241 ctttccgcag gagcaccagc cctcggccga gacgctgcac gcgatccggc tcgatctcgc
     3301 cgccgcgtgg cgggagttcg cgctgacgct caatgcccgg ctgctgccgc gcagcgtcgc
     3361 ctgaaacgaa ggccgcgccg gccgccggcc cggccgcgcc gacagggctg cgccggcctg
     3421 cgcggcatcg tctattacaa acccgatccg tccgccgagt agcctgaggg ctgcaagcgc
     3481 gaccgggtcc ggggcggctg agcctgcagc gccctgccgc ctgaatccct gacgatcccg
     3541 ccgcggccgc agaccccggt gcgcctgcgg ctgtccctgc cggagcccca gccatgcccg
     3601 aagtcatcgg catcgatcac ctctacatca gcgtcagcga tccggcgcgt gccgaggatt
     3661 tctacgaccg cgccttcgtg accgcgctcg gctttcgcaa gaaccgcttc gcgctcgatg
     3721 gcgagccgca cgtgcagtac tacaaccgcc acttcggcta cgtgctgcgc ccggcgcgca
     3781 gcccggcgac gcacgatccg tacgcgccgg gcctgcatca cttctgcctg cgcgtcgatt
     3841 cgatcgagga cgtggttgcg gcggccgggc ggctgcgcgc ggccggcatc gaggccagcc
     3901 cggccaccca ccatccgcaa tacgcgccgg actactgggc gtgcttcttc agcgacccgg
     3961 acggcatccg gctggaaatc accaacttcc ggcaggagcg gcgcgagcgt cacgcgcagg
     4021 gcgcagtcag cgattgacgc agtgtgcaag cgcagcaaca acatttgccg gaatgtggta
     4081 atcagtcaat aatcaggtac ttcaagcctg ccggtaagga tgtcatacac gtccgccagc
     4141 gctttgcccg cggacaccca cacccggcgc ccaggtgccg atccagggat gcaccatgac
     4201 cgacaatggc gactccccag accgccctcc ctatcgcccg ccggctttcg cgtcgacgga
     4261 tgcccggagc gatgccggca gcagcgcgtt tccgtggctg ctgttcctga tcgtgctgct
     4321 gccgctcggc ctgctgatgc tcggcgtcgc ggcctacgtc ggccatgccc gcatcgacgc
     4381 gatgattgcc gtcgaacgcg ccaataccgg catcgccgtc ggggtcgtgt cggggcggct
     4441 gaatgacgaa ctgcgcgtgc cggtgcgaca cctgcaggcg ctggccggcg agctgccggt
     4501 gcgcagtgcc atcaccagcg ccgaaccggg cgcggccgga ctggatgcag ccttcctgtc
     4561 gctgatggag cgcaacccga actatgacca ggtgcgctgg ctcgacgccg gcggacgcga
     4621 gcgggtgcgc ctgcagcgcg agggcggaca ggtgcagcgg gtcgcaagcg tggcgctgca
     4681 ggacaagagt gatcgtccgt acttccgcga ggcgatccgg ctggaggccg gcgagatgta
     4741 cgtgtcgccg ctggatctca acatcgagca tggccgcatc gaggagccgt acaagccgat
     4801 gctgcgcgtc gcgacgccgg tcgacgatgc cgagggcgtg cgccgcggcc tgctgatcat
     4861 caacgtgctg gcgcagccga tcctcgatgc cgccgagggc agcctcggca gcagcggcaa
     4921 tccggtgatg ctgctcaatg ccgacggcta cagcctgcgc agccccgagc cggcgcagga
     4981 cttcggcttc atgttcgggc gcagcgagcg cttcgcgacc cggcacgccg gcatctgggc
     5041 gcagatgacg gagacgccgg ccggacaggt cgaggacgcc gaagggctgt ggacctggcg
     5101 gcgggtcagt ctcgccagca cggcgggcaa gacgctggcc ggcacggcac cggagttcat
     5161 cgcgctgacg caggtgccag gcgcacggct ggtgcagcag cgtcgcgagg tctggctggc
     5221 ggtcggcggc atcaccgccg cgctgctgct cgcgcatacg ctgctgtcgc tggcgctggc
     5281 cttcgcgctg gccgggcgct cgcgcgccga aaccgggggg ctgtccac
//
LOCUS       DAOQIE010000269         5296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8828_length_5296_cov_7.883419, whole genome shotgun sequence.
ACCESSION   DAOQIE010000269 DAOQIE010000000
VERSION     DAOQIE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8828_length_5296_cov_7.883419"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PK144_14625"
     CDS             complement(<1..518)
                     /locus_tag="PK144_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HNF67008.1"
                     /translation="MNALPAAAAGLAAPATVPAPGLYVGTVRHRRFAPRAHAFRYPLC
                     MAFVDVDDPDAAFAGRWLWSARRPALAWFRREDHFDLGDPGEPLGDCVRRQVERETGR
                     RPDGAIRLLTQLRWFGHCFNPISLYYCYGAGGGLVALLAEVTNLPWGERHCYVLDAPA
                     WGRVLLAGLVVLA"
     gene            complement(515..1786)
                     /locus_tag="PK144_14630"
     CDS             complement(515..1786)
                     /locus_tag="PK144_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF67009.1"
                     /translation="MRYAIVGSGISGLTVAHRLHGRHGQHEFTLYEAGDHVGGHTHTH
                     DVHVGGRGFAVDTGFIVFNERTYPNFIALLDELGVASRPTEMSFSVRCAASGLEYNGH
                     DLNSLFAQRRNLVSPRFWRMLAAILRFNREAPLVLADGTDAHLTLGQYLQRERYPREF
                     IDWYIVPMAAAIWSARPEVMTEIPVLTFVRFFRNHGLLAVRDRPQWRTVCGGSREYVR
                     RLTAPFSGRIRLHTPVTGVRRHAGGVQVLTRAAEPERYDGIIFACHADEALAILGPEA
                     TPAERAVLGAIHYQPNEAVLHTDAALLPHCARARASWNYHLDGQPRTRVPVTYDMARL
                     QGLDCPLPLLVTLNRSERIDPAKVLRRIGYRHPVFTPEAILAQQRRAEISDRLRSWYC
                     GAYWGYGFHEDGVVSALAVLDELDRHQAVAA"
     gene            complement(1804..2232)
                     /locus_tag="PK144_14635"
     CDS             complement(1804..2232)
                     /locus_tag="PK144_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF67010.1"
                     /translation="MAMLLERFKSLFNELDAGCLDRLAEVYAADVRFRDPVRELHGLE
                     ALRAYYARLYEGVESCRFRYDGEVVEAGGAVVLWTMQFRHRRFHAGETLTLEGASHLE
                     FGQGADARVHRHRDYFDMGEFLYERVPVLGAVIRHIKSGL"
     gene            complement(2654..2968)
                     /locus_tag="PK144_14640"
     CDS             complement(2654..2968)
                     /locus_tag="PK144_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67011.1"
                     /translation="MNLLTRTAATSLLLALSAGSALADCDGRTGSLMSIPTPVAGSED
                     LPPALAELIAHHRGEIGAPEVSTTQHVQPQTPPAPAVAVLPPNTRVALSGPAATLPST
                     LH"
     gene            3291..4064
                     /locus_tag="PK144_14645"
     CDS             3291..4064
                     /locus_tag="PK144_14645"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNF67012.1"
                     /translation="MNARLAAVLIGLCASTGTLPAPADVTLTYALHDDGDGGSQIIAI
                     AGSRLRFETVQEGERAIVLYDGRRHVLTMLDAEQKAYVEFGESSAAELERASAERQRA
                     SDARLGEELAKLPPEERAALEASLRGNVATEAVRVPRRIEQTGQHRSVAGIGCGIVNV
                     WVDDEKQEAHCVASREALGLSEEEFATLTGALSALEQSSGQDGSVAAALGGVPIHSTE
                     RGEEDSELVTELRSVSRAAVPAERLRVPPDYQQQEAPPP"
     gene            4167..>5296
                     /locus_tag="PK144_14650"
     CDS             4167..>5296
                     /locus_tag="PK144_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF67013.1"
                     /translation="MYETSFGFTRSPFLDPEGSEFRPCGAQAAVLAELREALDAGAAL
                     VVLSGPIGTGKTTLLRRALGEPMRQRHVVLFWNAHVGPDDLLNYLCDDLDLRSPGTGR
                     TERLKALRTALVRGLSEGWSLLIAVDDAQNLPAETLAELHALLDLREVGHGLVQVLLC
                     GQPELQDRLVHDPQRDAHPPRRLTLRTLDATESAAFIAARLAAAGRPDADLFDAEALV
                     RIHELAHGVPRLLNRICNQSLLLAYLGSERRVSAALVRDVAADTPPTEPPPQPAGPLY
                     EAMPARPVIAADSVPHMHAPVHESVPAAVTGPGNAPAPVQAASAHLHADPHAHGHSHR
                     SSRRRSRWWIGLAAVLSALALAGFAAWRNGWLPGLPPPRPAAP"
BASE COUNT          805 a         1890 c         1892 g          709 t
ORIGIN      
        1 gccaggacca ccagacccgc gagcagcacc cggccccagg cgggggcgtc gagcacgtag
       61 cagtggcgct cgccccaggg caggttcgtg acctcggcga gcagcgcgac gaggccgccg
      121 ccggcgccgt agcagtaata aaggctgatc gggttgaagc agtgaccgaa ccagcgcagc
      181 tgcgtcagca gccggatcgc cccgtcgggc cgccggccgg tctcgcgctc gacctgccgg
      241 cgcacgcagt cgccgagcgg ctcgcccgga tcgccgaggt cgaagtgatc ctcgcgccgg
      301 aaccacgcga gcgccggccg gcgcgccgac cacagccagc gcccggcgaa ggcggcatcg
      361 gggtcatcga catcgacgaa ggccatgcag agcgggtagc ggaacgcatg cgcgcgcggc
      421 gcgaagcgcc ggtggcggac cgtgccgacg tacagacccg gagccggcac cgtggccggg
      481 gccgcgaggc cggcagccgc agcgggcagc gcgttcatgc ggccaccgcc tggtggcggt
      541 cgagctcatc gagcacggcc agcgcgctga cgacgccgtc ctcgtggaag ccgtaccccc
      601 agtaggcacc gcagtaccag ctgcgcaggc ggtcgctgat ctcggcgcgc cgctgctgcg
      661 ccagaatcgc ctcgggcgtg aacaccggat ggcggtagcc gatgcgcctg agcaccttgg
      721 ccggatcgat ccgttcgctg cggttcagcg tcaccagcag cggcagcgga cagtcgaggc
      781 cctgcagccg ggccatgtcg taggtcaccg gcacgcgcgt gcgcggctgg ccgtcgaggt
      841 ggtagttcca cgaggcgcgc gcgcgtgcgc agtgcggcag cagcgcggcg tcggtgtgca
      901 gcaccgcctc gttcggctgg tagtggatcg cgccgagcac ggcgcgctcg gccggcgtcg
      961 cctcggggcc gaggatcgcc agcgcctcgt cggcgtggca cgcgaagatg atgccgtcgt
     1021 agcgctcggg ctcggctgcg cgcgtcagca cctgcacgcc gccggcgtgg cggcgcacgc
     1081 cggtcaccgg cgtgtgcagg cggatgcgcc cggaaaacgg cgccgtcagc cggcgcacgt
     1141 actcgcgcga gccgccgcag accgtgcgcc actgcggccg gtcgcggact gcgagcaggc
     1201 cgtggttgcg gaagaagcgc acgaaggtca gcaccgggat ctcggtcatg acctcggggc
     1261 gcgccgacca gatcgccgcc gccatcggca cgatgtacca gtcgatgaac tcgcgcggat
     1321 agcgctcgcg ctgcaggtac tggccgagcg tcaggtgcgc gtcggtgccg tccgcaagca
     1381 ccagcggtgc ctcgcggttg aagcgcagga tcgccgcgag catgcgccag aagcgcgggc
     1441 tgacgaggtt gcgccgctgc gcgaacaggc tgttgaggtc gtggccgttg tattcgaggc
     1501 cgctcgccgc gcagcgcacg ctgaagctca tctcggtcgg gcgcgaggca acgccgagct
     1561 catcgagcag cgcgatgaag ttcggatacg tgcgttcgtt gaagacgatg aagccggtat
     1621 cgaccgcgaa gccgcgcccg ccgacgtgca cgtcgtgcgt gtgcgtgtgg ccgccgacgt
     1681 gatcgccggc ttcgtacagc gtgaattcgt gctgtccgtg ccgcccgtgc agccggtggg
     1741 cgacggtcag gccggaaatg ccgctgccga cgatcgcgta gcgcatggct cgccctcccc
     1801 cgctcacagc ccggatttga tgtggcggat caccgcaccg agcaccggca cgcgctcgta
     1861 caggaattcg cccatgtcga agtagtcgcg gtgacggtgc acgcgcgcat cggcaccctg
     1921 gccgaattcg aggtggctcg cgccttcgag cgtcagcgtc tcgccggcgt gaaagcggcg
     1981 gtggcggaac tgcatcgtcc acagcacgac cgcgccgccg gcctcgacca cctcgccgtc
     2041 gtagcggaag cggcacgatt cgacgccctc gtacagccgc gcgtagtagg cgcgcagcgc
     2101 ctcgaggccg tgcagctcgc ggaccgggtc acggaagcgc acgtcggcgg cgtagacctc
     2161 ggccagacgg tcgaggcagc cggcgtcgag ttcgttgaac agcgacttga agcgttcgag
     2221 gagcatggcc atgcgggtca cctgcaaacc tgcaagacgg tgtttcttcg ccggccggtg
     2281 tgcggcgctg cctgcgatcg ccaccgactt cgacacaacc gacgctgagc gtcggaattg
     2341 gttcaacaaa acctatacaa aaaagccata caaacaaaac ctctacaaac cgacaggtgc
     2401 cggcttacag gcgatgtgcg tgccgccggc ggccgcggat gcagccgggc gaaaaaaacc
     2461 tgcgcccccg cccccgcgcg cccggccgga cgcccaaaag aaaccgccgt cggcggccgg
     2521 gcggggaacc ggcagacgac ggcggcgggt gcgtgcgagg gagaagcggt gcggcaggga
     2581 aacggtggcg ctgggcgctc tggtgcgggt acggctcagg tccggtgcgg ggggatggga
     2641 aaccggctcg aattcagtgc agcgtggacg gcagcgtggc ggcggggccg gacagggcga
     2701 cgcgcgtgtt cggcggcagc accgcgaccg ccggtgccgg cggtgtctgc ggctggacgt
     2761 gctgcgtggt cgagacttcg ggggcgccga tctcgccgcg gtgatgagcg atcagctcgg
     2821 ccagcgccgg cggcagatcc tcgctgcccg cgaccggggt cggaatcgac atcagcgagc
     2881 cggtccggcc atcgcagtcg gccagcgcgc tgccggcgga cagggcgagg aggagggagg
     2941 tagcagcggt gcgggtcaga aggttcatca cgatgctcct gctgtcaggg gcgcagcgag
     3001 tgcgcagtag cgtttgtaca gatcatctac accgaccttc ccccgatgct tcgtctgatt
     3061 tccggcccct gaagatcagc ttttcgggcg atgccaatgt ggacaaaacc tgtatagcgc
     3121 agtcacttgc tcaatgacaa ttttccacga ttaaaatttg aacagattct gtactgcttc
     3181 cgtccccggt cccggcgatc cgcggccacc gctccgctct tgggcggccg gtcgataccg
     3241 gctactgtgg acgcagctcc ttcttccttt ccctcccagg tagtcccgcc atgaatgcac
     3301 gcctcgcggc ggtgctgatc ggactgtgcg ccagcaccgg cacgctgccc gcgcccgccg
     3361 acgtcacgct gacctatgcc ctgcacgacg atggggacgg cggctcccag atcatcgcga
     3421 tcgccggcag ccggctgcgc ttcgagaccg tgcaggaagg ggagcgcgcg atcgtgctgt
     3481 acgacggcag gcgccacgtg ctgaccatgc tcgatgccga acagaaggcc tacgtcgagt
     3541 tcggcgaaag ttcggccgcc gaactcgaac gcgccagcgc cgagcgccag cgcgccagcg
     3601 atgcccgcct cggcgaggaa ctcgcgaagc tgccgcccga ggagcgcgcc gcgctcgaag
     3661 ccagcctgcg cggcaacgtc gcgaccgagg ccgtgcgggt gccgcggcgc atcgagcaga
     3721 ccggccagca ccgcagcgtc gccggcatcg gctgcggcat cgtcaacgtc tgggtcgatg
     3781 acgagaagca ggaagcccac tgcgtcgcca gccgcgaggc gctcggcctc agcgaggagg
     3841 aattcgcgac gctgaccggc gcattgtcgg cgctggagca gtcatccggt caggacggca
     3901 gcgtcgccgc cgccctcggc ggcgtgccga tccattccac cgagcgcggc gaggaggact
     3961 cggaactcgt caccgaactg cgcagcgtgt cgcgcgccgc ggtgccggcg gaacgcctgc
     4021 gtgtgccgcc tgactatcag cagcaggaag cgccgccgcc ctgagccgca gcgcgccgcc
     4081 atggatcgtg acgcggcgca tgccccgtcc ggcagacccg gcgctccccg cccgctcccc
     4141 tgccctgccg ccgacgattc cgccccatgt acgaaaccag cttcggcttc acgcgctccc
     4201 cgttcctcga tcccgagggc agcgaattcc gcccctgcgg ggcgcaggcc gccgtgctgg
     4261 ccgaactgcg cgaggcgctc gacgccggtg cggccctggt ggtgctcagc ggcccgatcg
     4321 gcaccggcaa gaccacgctg ctgcgccgcg cgctcggcga gccgatgcgg cagcgccacg
     4381 tcgtgctgtt ctggaacgcg cacgtcggcc ccgacgacct gctgaactac ctctgcgacg
     4441 acctcgacct gcgctcgccc ggcaccggcc gcaccgagcg gctgaaggcg ctgcgcacgg
     4501 cgctggtgcg cgggctgtcc gaaggctggt cgctgctgat cgccgtcgac gacgcccaga
     4561 acctgccggc cgagacgctc gccgagctgc acgcgctgct cgacctgcgc gaggtcggcc
     4621 acggcctggt gcaggtgctg ctgtgcggcc agccggagct gcaggatcgg ctcgtgcacg
     4681 atccgcagcg cgacgcccac ccgccgcggc gcctgacgct gcgcacgctc gatgccaccg
     4741 agagcgcggc cttcatcgcc gcacgcctgg ccgcggccgg ccggcccgat gccgatctgt
     4801 tcgatgccga agccctcgtg cgcatccatg aactcgcgca cggcgtgccg cggctgctga
     4861 accgcatctg caaccagtcg ctgctgctcg cctacctcgg ctccgaacgc cgggtctcgg
     4921 cggcgctggt gcgcgacgtg gccgccgaca cgccgccgac cgagccgcca ccgcagcctg
     4981 ccggtccgct gtacgaagcc atgccggcac ggccggtgat cgcggccgac tcggtgccgc
     5041 acatgcatgc gcccgtgcac gagagcgtgc cggccgccgt caccggcccg ggcaatgcgc
     5101 ccgcgccggt gcaggcggcg agcgcacacc tgcacgccga tccgcacgcc cacggtcaca
     5161 gccaccgctc gtcacgccgg cgcagccgct ggtggatcgg gctggccgcg gtgctgagcg
     5221 cgctggccct ggccggcttc gcggcctggc gcaacggctg gctgcccggg ctgccgccgc
     5281 cccggccggc ggcgcc
//
LOCUS       DAOQIE010000270         5290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8843_length_5290_cov_7.950143, whole genome shotgun sequence.
ACCESSION   DAOQIE010000270 DAOQIE010000000
VERSION     DAOQIE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8843_length_5290_cov_7.950143"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1184)
                     /gene="apbC"
                     /locus_tag="PK144_14655"
     CDS             complement(93..1184)
                     /gene="apbC"
                     /locus_tag="PK144_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNF67014.1"
                     /translation="MSSLTRPMVDAAIQAYVDPYLDQDLIAAKCLRDVRIDGGSVAVA
                     IELGFPSGEYPAKLAGELETRLRALPGVEAATVTVTTKIIAHEVQKGLKPLPGVKNVI
                     AVASGKGGVGKSTTSVNLALALAADGARVGLLDADIYGPSQPRMLGAMQQPESADGKS
                     IEPVIAHGVQSMSIGYLIEEETPMIWRGPMATQALEQLLRDTRWKDLDYLVIDLPPGT
                     GDIQLTLSQKVPVSGAVIVTTPQDIALLDARKGLKMFEKVEVPILGIVENMSIHICSN
                     CGHAEHIFGAGGGERMAAQYDTKFLGSLPLDIRIREQADNGHPTVAVDPDGQVAGLYR
                     HIARRAAARLALSAKDYSHRFPNIVIQKN"
     gene            complement(1310..1540)
                     /locus_tag="PK144_14660"
     CDS             complement(1310..1540)
                     /locus_tag="PK144_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNF67015.1"
                     /translation="MPLFDFRCPACEVTFELLVRSSTVPACPHCGSQTLDRQVARIAP
                     AATTPGLLRNARKVAMKEGHFSNYKPSERPKV"
     gene            1761..2594
                     /locus_tag="PK144_14665"
     CDS             1761..2594
                     /locus_tag="PK144_14665"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNF67016.1"
                     /translation="MDMPELLKALILGLVEGATEFLPVSSTGHQIIVADLLDFTGERA
                     KTFEIFIQLGAILAVIWYYRAKIGHVLSTLGSESASRRLIANLLVAFLPAAVLGLAFS
                     KVIKAHLFNPVTVATALIVGGFIILLIEARKRPATISSTDQISGRTALMIGIAQCFAL
                     IPGTSRSGATIMGALLCGLSRPAAAEFSFFLSIPTMIAATLYSLYKARDVLSSTDIPT
                     FGVGFVAAFVSGFIVVKLFLAFVSKHSFNAFAWYRIALGSAVIAYFYFTGQPMFGVLT
                     D"
     assembly_gap    2595..2694
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2749..3240)
                     /locus_tag="PK144_14670"
     CDS             complement(2749..3240)
                     /locus_tag="PK144_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNF67017.1"
                     /translation="MADLLKNRARVTDPFVSVADDAPLPAAGTPVLVTLARWQAERDA
                     LIARGDAIGVQVPGTTTAAELAADLGHWALVAIEFPVFKDGRGYTLARLLRERFAYPG
                     ELRAVGDVLRDQLFFLSRAGFDAFALRADQDFDEALTAFDDFTVRYQPAADEPLPLYR
                     RRG"
     gene            complement(3233..4924)
                     /locus_tag="PK144_14675"
     CDS             complement(3233..4924)
                     /locus_tag="PK144_14675"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNF67018.1"
                     /translation="MYQYDSHDQRLVDERVAQFRGQVRRYLAGQLSDDEFRPLRLMNG
                     LYLQTHAYMLRIAVPYGLLSSKQMRMLAHIARTWDRGYGHFTTRQNIQFNWPKLEDVP
                     EILAALASVQMHAIQTSGNCIRNVTTDHLAGVAEDELEDPRLWCEIIRQWSTLHPEFS
                     YLPRKFKIAVSATAADRAATQVHDIGLYLTRNDAGETGFQVLVGGGLGRTPIIGQVIR
                     EFVAPRDLLSYLEAILRVYNLEGRRDNLTKARIKILVKALGIDEFRRRVDAEWAEIRE
                     SGLVLDEAEIERVRAFFTPPAYEASAAEDASWTGLRDADPAFARWLKRNTAGHKVAGY
                     RNVFVALKPFGQAPGDITAEQMDAVADLADRYSFGQLRSTHTQNLILADVRNADLHAL
                     WQALDALGLATPNIGTIADIICCPGLDFCSLANAGSIGVAKSIQSRFEDLDYLYDLGD
                     LRLNISGCMNSCAHQHVGAIGVLGVDKGGEEWYQITIGGSSGRDASLGDVIGPSVAKD
                     AVADTVEKLVAVYVEQREPEEPFLATVRRIGVKPFKERVYGAKSASARKPETVNG"
     gene            complement(5118..>5290)
                     /locus_tag="PK144_14680"
     CDS             complement(5118..>5290)
                     /locus_tag="PK144_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018945715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNF67019.1"
                     /translation="TALLNQAKQARESASGVNLDEEAADLMRFQQAYAASAQVMKTAD
                     ELFQTLLGIARG"
BASE COUNT          810 a         1720 c         1764 g          896 t
ORIGIN      
        1 ggcgggccgg atcagatgaa ggaagggcgc gcgattctaa ccgcgcgccc ttcttatcct
       61 gatctgaaat gtgcctgacg cgcgatccgg cttcagttct tctggatgac gatgttcggg
      121 aagcggtgcg agtagtcctt cgccgacagc gccagccgcg ccgcggcccg gcgggcgata
      181 tggcggtaga ggccggcgac ctgtccgtcc ggatcgacgg cgacggtcgg gtggccgttg
      241 tcggcctgct cgcggatgcg gatgtcgagc ggcaggctgc cgaggaactt ggtgtcgtac
      301 tgcgcggcca tgcgctcgcc gccgccggca ccgaagatgt gttcggcgtg accgcagttc
      361 gagcagatgt ggatgctcat gttctcgacg atgccgagga tcggcacctc gaccttctcg
      421 aacatcttca ggcccttgcg cgcatcgagc agcgcgatgt cctgcggcgt cgtgacgatc
      481 acggcacccg agaccggcac cttctgcgac agcgtcagct ggatgtcgcc ggtgcccggc
      541 ggcaggtcga tgaccagata gtcgagatcc ttccagcgcg tgtcgcgcag cagctgttcg
      601 agcgcctgcg tggccatcgg gccgcgccag atcatcggcg tctcttcctc gatcagatag
      661 ccgatcgaca tgctctgcac gccatgcgcg atgaccggct cgatcgactt gccgtcggcc
      721 gactcgggct gctgcatcgc gccgagcatg cgcggctgcg acggaccgta gatgtcggca
      781 tcgagcaggc cgacgcgcgc accgtcagcg gcgagcgcca gcgccagatt gaccgaggtc
      841 gtcgacttgc cgacaccgcc cttgccggag gcgacggcga tgacgttctt gacgccgggc
      901 aggggcttga gacccttctg cacctcgtgc gcgatgatct tcgtcgtgac cgtgaccgtg
      961 gcggcctcga cgccgggcag cgcacgcagc cgggtttcga gttcgccggc gagcttcgcc
     1021 gggtactcgc cgctcgggaa gccgagttcg atcgcgacgg ccacgctgcc gccgtcgatg
     1081 cgcacgtcgc gcaggcactt ggcggcgatc agatcctgat cgaggtaggg gtccacatag
     1141 gcctggatgg cggcatccac catcggccgg gtcaggctgc tcatcacgac tactccgggc
     1201 ggtttcagaa acgacttgcg cagccggtgc ggctgcggcg ggatgcggga ttgtatccga
     1261 cgtatccgac cgctgcgctc ggaaaacccg gcttgcatcc gccgcaggct cagaccttcg
     1321 gacgctccga cggcttgtag ttgctgaagt gcccttcctt catcgccacc ttgcgcgcgt
     1381 tgcgcagcag ccccggcgtc gtggccgccg gtgcgatgcg ggcgacctgc ctgtccagcg
     1441 tctggctgcc gcagtgcgga caggccggca cggtgctcga acgcaccagc agttcgaagg
     1501 tgacctcaca ggccgggcag cgaaaatcga acagcggcat gaaatcctcc cttgtcgcga
     1561 acccgacaga agtgacaaag caacggggtg tgcacaggat ggatgcaagc cggatgccga
     1621 tcacgccctg ctcgccgccg ccgcgccgcc gcgccgccgc gccgccggca ggattcacgg
     1681 cgcgacaagt ccggcgaggc ggcgcatccc cgtagaatgc gcgccgtgtc ccacccgact
     1741 gccacgaacg aggaactgcc ttggacatgc cggaactgct gaaagccctg atcctgggcc
     1801 tggtcgaagg cgcgaccgaa ttcctgccgg tgtcgagcac cggccatcag atcatcgtcg
     1861 ccgacctgct ggacttcacc ggcgaacgtg ccaagacctt cgagatcttc atccagctcg
     1921 gcgcgatcct cgcggtgatc tggtactacc gcgcgaagat cggccatgtg ctctcgacgc
     1981 tcggcagcga atcggcatcc cggcgcctga tcgccaacct gctcgtcgcc ttcctgccgg
     2041 ccgccgtgct cgggctggcg ttcagcaagg tgatcaaggc gcacctgttc aacccggtca
     2101 cggtcgccac ggcgctgatc gtcggcggct tcatcatcct gctgatcgag gcgcgcaagc
     2161 gcccggcgac gatcagctcg accgaccaga tcagcggccg cacggcgctg atgatcggca
     2221 tcgcccagtg cttcgcgctg atccccggca cctcgcgctc gggcgcgacg atcatgggcg
     2281 cgctgctctg cgggctgtca cgaccggcgg cggccgagtt ctcgttcttc ctgtcgatcc
     2341 cgaccatgat cgccgcgacg ctgtactcgc tgtacaaggc gcgcgacgtg ctttcgagca
     2401 ccgacatccc gaccttcggc gtcggcttcg tcgccgcctt cgtctccggc ttcatcgtcg
     2461 tgaagctgtt cctcgccttc gtgtcgaagc acagcttcaa cgccttcgcg tggtaccgga
     2521 tcgcgctcgg ctcggcggtg atcgcgtact tttacttcac cgggcagccg atgttcgggg
     2581 tgctgaccga ctgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncatgaa
     2701 aaaagcccgg ttcacaccgg gcttttttgt ctgcaccgct gcggcgtttc agccgcggcg
     2761 ccggtacagc ggcagcggtt cgtcggcggc cggctggtag cggaccgtga agtcgtcgaa
     2821 ggcggtcagg gcttcgtcga agtcctgatc ggcgcgcagc gcgaaggcgt cgaagccggc
     2881 gcgtgacagg aagaacagct ggtcgcgcag cacgtcgccg accgcacgca gctcgcccgg
     2941 ataggcgaaa cgctcgcgca gcagccgcgc cagcgtgtag ccgcggccgt ccttgaacac
     3001 cgggaactcg atcgcgacca gcgcccagtg tccgaggtcg gcggcaagct ccgccgccgt
     3061 cgtcgtgccc ggcacctgca cgccgatggc atcgccacgg gcgatcagcg catcgcgctc
     3121 ggcctgccag cgggccagcg tcaccagcac cggcgtgccg gcggccggca gcggggcatc
     3181 gtcggcgacg ctcacgaacg ggtcggtgac gcgcgcgcgg ttcttgagga ggtcagccat
     3241 tgacggtctc cggcttgcgg gcagaagcgg acttcgcgcc gtagacgcgc tccttgaagg
     3301 gtttgacgcc gatgcgacgc acggtggcga ggaagggttc ttccggctcg cgctgctcga
     3361 cgtagacggc gacgagtttt tcgaccgtat cggcgaccgc gtccttcgcg accgacgggc
     3421 cgatcacgtc gccgagcgag gcatcgcgcc cggagctgcc gccgatcgtg atctggtacc
     3481 actcctcccc gcccttgtcg acgccgagca cgccgatggc gccgacgtgc tggtgcgcgc
     3541 aggagttcat gcagccggag atgttcaggc gcaggtcgcc gaggtcgtag aggtaatcga
     3601 ggtcctcgaa gcggctctgg atgctcttgg ccacaccgat cgaaccggcg ttggcaaggc
     3661 tgcagaaatc gaggcccggg cagcagatga tgtcggcgat cgtgccgatg ttcggcgtcg
     3721 ccagcccgag cgcatcgagg gcctgccaca gcgcgtgcag gtcggcgttg cgcacgtcgg
     3781 caaggatcag gttctgcgtg tgcgtcgagc gcagctggcc gaagctgtag cggtcggcga
     3841 gatcggcaac cgcgtccatc tgctcggccg tgatgtcgcc gggcgcctga ccgaagggct
     3901 tcagcgcgac gaacacgttg cggtagccgg cgaccttgtg gccggcggtg ttgcgcttca
     3961 gccagcgcgc gaaggccgga tcggcatcgc gcagcccggt ccaggacgca tcttccgcag
     4021 cactggcctc atacgccggc ggcgtgaaga acgcgcgcac gcgctcgatc tcggcctcgt
     4081 cgagcaccag cccggattcg cggatctcgg cccactcggc gtcgacgcgc ctgcggaact
     4141 cgtcgatgcc gagcgccttc acgaggattt tgatgcgcgc cttggtcagg ttgtcgcgcc
     4201 ggccttcgag gttgtagacg cgcaggatgg cttcgaggta gctcagcagg tcgcgcggcg
     4261 cgacgaactc gcggatcacc tggccgatga tcggcgtgcg cccgaggccg ccgccgacca
     4321 gcacctggaa gccggtttcg ccggcgtcgt tcctcgtcag gtacaggccg atgtcatgga
     4381 cctgggtcgc ggcgcgatcg gcggccgtgg cgctgaccgc gatcttgaac ttgcgcggca
     4441 ggtaggagaa ttccgggtgc agcgtcgacc actggcggat gatctcgcac cagagccgcg
     4501 ggtcttcgag ttcgtcctcg gcgacaccgg cgaggtggtc ggtggtgacg ttgcggatgc
     4561 agttgccgct ggtctggatc gcgtgcatct gcaccgaggc gagcgcggcg aggatctccg
     4621 gcacgtcttc gagcttcggc cagttgaact ggatgttctg gcgggtcgtg aagtgaccgt
     4681 agccgcgatc ccaggtgcgg gcgatgtgcg cgagcatgcg catctgcttc gacgacagca
     4741 gaccgtacgg caccgcgatg cgcagcatgt aggcgtgcgt ctgcaggtac aggccgttca
     4801 tcagacgcag cggccggaac tcgtcgtcgc tgagctggcc ggcgagatag cgccggacct
     4861 gcccgcgaaa ctgcgcgaca cgctcgtcaa cgagccgctg gtcgtgggaa tcgtactggt
     4921 acatgggggc gatcacctgt cgaaagcgag cgccggacgg cttgtccggc gatcaggcgc
     4981 gcgacggtaa cagcgtccca tatattcttg aagcattatt ttttgatact taaattcctg
     5041 attgaaataa gcaaccggaa tatcccgcct gtcgcctccg gcatccgccg cgcgctgccg
     5101 cccacccggg ctgcgcatca gccgcgggcg atgccgagca gggtctggaa cagttcgtcg
     5161 gcggtcttca tgacctgcgc cgaagccgcg taagcctgct ggaagcgcat caggtcggcg
     5221 gcttcctcgt cgaggttgac gccggaagcc gattcacgcg cctgcttcgc ctgattcagc
     5281 agcgccgtct
//
LOCUS       DAOQIE010000271         5275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_8886_length_5275_cov_7.815900, whole genome shotgun sequence.
ACCESSION   DAOQIE010000271 DAOQIE010000000
VERSION     DAOQIE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8886_length_5275_cov_7.815900"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="arsS"
                     /locus_tag="PK144_14685"
     CDS             complement(<1..836)
                     /gene="arsS"
                     /locus_tag="PK144_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164889.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNF67020.1"
                     /translation="MHATRPLLDAIDFPALRRRRLETVQANLGYRCNQACLHCHVNAG
                     PTRREAMSRDTAEAVLELVAASGATTLDLTGGAPELNPHFRWLVETAHARGVQVMDRC
                     NLTILSEPGHEDLADFLAAHRVEVVASLPCYLEDNVDGQRGAGVYARSIAGLRQLNAL
                     GYGDGRSGLMLNLVYNPTGPYLPPPQAALEADYRRELGGRHGIVFDRLFTLANMPIQR
                     FGSTLVSHGEFDAYMTLLRGAHRDDNLDQVMCRTLVSIGWDGTVYDCDFNQMLGLPLA
                     VPG"
     gene            complement(884..2512)
                     /locus_tag="PK144_14690"
     CDS             complement(884..2512)
                     /locus_tag="PK144_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNF67021.1"
                     /translation="MSLPLAILVLLFAVLACGAVSRRSAMPLPLLLIPAGTLLALVPG
                     MHAVAPDPETFFLVFVPPLLFADAWLIPKREFLNLRYPILGLALGLVLCTVLATGYLV
                     HWLIPSVPLAAGFALGAVISPTDAVAVSSITERLRLPARLTHLLNGESLINDASGLVA
                     FRFSVAAALTGSFSAGAAAANFMLVAVGGVLAGLAATWVLGRLRRGLSHRGWADESIS
                     TGLTLLAPYASYLAAESLHLSGVLGAVAGGWYAAMQEARDTKPSERLVMLNVWQLLLF
                     FFNGLVFVLLGLRLPEVLEGVAGQPWPMLAGGALAITAVAMLIRLVWVFPASRAMLWL
                     TRRHAPQTPVPPWRQVLVAGWAAPRGAITLAAALSLPAGGFPERELLVFYASAVIVLT
                     LVVNALTLPALIRRLGLRADDVQLREMRSAHVALAQAAIRRLRAALDLQHAEHEMRYT
                     QRLIEDYERRIGRHCGRSAEEAMRIAPEFETEISLRRLGIAAERRELRRLRREALISD
                     EVIRDLLHDLDLREVGLDSVARQPSAASARAAEA"
     gene            complement(2672..4021)
                     /gene="chrA"
                     /locus_tag="PK144_14695"
     CDS             complement(2672..4021)
                     /gene="chrA"
                     /locus_tag="PK144_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNF67022.1"
                     /translation="MPPAPDRSDALTAPPAPGFGEALRYWLRLGCISFGGPAGQIAIL
                     HQDLVEHRRWISESRFLHALNYCMVLPGPEAQQLATYIGWLLHGTKGGIAAGALFVLP
                     SVFVLIALSWIYLAFGQVPAVAGVLWGIKPAVTAIVVFAAWRIGSRALKNPVLWAIAA
                     AAFLGIAVFDLPFPLIVIGAGLIGALGGRLAPQCFRGGGGHGAGKASYGPALIDDHTP
                     TPAHARIDRGRLTRLALGGFGLWAGGLGLLALVFGGVLVDMGLFFTKAALLTFGGAYA
                     VLPYVYQGAVEHFHWLTGAQMIDGLALGETTPGPLIMVVAFVGYVGAWTKEVLGPDAL
                     AWAGALGAVVATVFTFLPSFLFILIGGPWVEATRGDLKFTAPLTGITAAVVGVILSLA
                     LFFAEHVLWPAGFGGRFEWLAAVIGAGALVALIRYKAGVIPVIGVCAGLGLLHGLVG"
     gene            complement(4298..5224)
                     /locus_tag="PK144_14700"
     CDS             complement(4298..5224)
                     /locus_tag="PK144_14700"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNF67023.1"
                     /translation="MSLWPIFDSLAPVFLLIALGAALRRSGFLSLTTLTGLNRLTYWI
                     GLPCLTVHDIATGPFQPLAAAGLFTTVLVATVIGGVLAWYCAGACGAGRAARGTFVQA
                     AFRGNLYFISLPVIVYASPGHPEVRSAALIASAPVILLYSIAAVLVLEASRSTPGVGR
                     AGLWRSLATNPQLLACVIGLALAASGAGLPPSLERSLDALGRMALPLALLGIGGSLIG
                     AHIGSRLRPVFGAALIKTALLPLLGFGFGWLLGLAPEQLRSALILLSTPTASASYVLA
                     RQLGGDEALASGSIVLSTLLSAPVLALVLALV"
BASE COUNT          885 a         1881 c         1925 g          584 t
ORIGIN      
        1 cccggcacgg cgagcggcag gccgagcatc tggttgaagt cgcagtcgta gaccgtgccg
       61 tcccagccga tcgacaccag tgtccggcac atgacctgat cgaggttgtc gtcgcggtgc
      121 gcaccgcgca gcagcgtcat gtaggcgtcg aactcgccgt gcgagaccag cgtcgagccg
      181 aagcgctgga tcggcatgtt ggcgagcgtg aacagccggt cgaagacgat gccgtggcgc
      241 ccgccgagtt cgcgccggta gtcggcttcg agcgcggcct gcggcggcgg caggtacggg
      301 ccggtcgggt tgtagaccag attcagcatc agcccggaac ggccgtcgcc gtagccgagc
      361 gcgttcaact gccgcaggcc ggcgatgctg cgcgcataca ccccggcgcc gcgctgaccg
      421 tcgacgttgt cttcgaggta gcacggcagc gaggcgacga cctcgacccg gtgcgcggcg
      481 agaaagtccg ccagatcctc gtggccgggc tcggacagga tggtcaggtt gcagcggtcc
      541 atcacctgca cgccgcgcgc atgggcggtt tcgaccagcc agcggaagtg cggattcagc
      601 tccggcgcgc cgccggtcag gtcgagcgtc gtcgcgccgc tcgcggcgac gagttcgagc
      661 acggcttcgg cggtgtcgcg cgacattgcc tcgcgccgcg tcgggccggc gttgacgtgg
      721 cagtgcaggc aggcctggtt gcagcggtag ccgaggttgg cctgcacggt ttcgagccgg
      781 cggcggcgca gcgccgggaa atcgatggca tcgagcagcg ggcgcgtggc gtgcatggcg
      841 gcgggtccct ggaatgcgtt ccggcggcgg ggggagcctg actttacgct tcggcggcgc
      901 gcgcggatgc cgccgagggc tggcgggcga cgctgtcgag gccgacctcg cgcaggtcga
      961 gatcgtgcag cagatcgcgg atgacctcgt cgctgatcag cgcctcgcgc cgcagccggc
     1021 gcagctcgcg ccgctcggcg gcgatgccga gccggcgcag gctgatctcg gtctcgaact
     1081 ccggcgcgat gcgcatcgcc tcctcggccg agcgaccgca gtggcggccg atgcgccgct
     1141 cgtagtcctc gatcagccgc tgcgtgtagc gcatctcgtg ctcggcgtgc tgcaggtcga
     1201 gcgcggcgcg cagccgccgg atcgccgcct gcgccagcgc gacgtgggcg ctgcgcatct
     1261 cgcgcagttg cacgtcatcg gcgcgcaggc cgagccggcg gatcagcgcc ggcagcgtca
     1321 gcgcgttgac gaccagcgtc agcacgatca ccgcgctcgc gtagaacacc agcagctcgc
     1381 gctccggaaa gccgccggcc ggcagcgaca gcgccgccgc cagcgtgatc gccccgcgcg
     1441 gggctgccca gccggccacc agcacctgcc gccacggcgg caccggcgtc tgcggcgcat
     1501 gccggcgcgt cagccagagc atcgcgcgcg aggccgggaa cacccagacc agccggatca
     1561 gcatcgccac ggccgtgatc gccagcgcgc cgccggcaag catcggccat ggctggccgg
     1621 cgacgccttc gagcacctcg ggcaggcgca ggccgagcag cacgaacacc agaccgttga
     1681 agaagaacag cagcagctgc cagacgttga gcatgaccag ccgctcggac ggcttggtgt
     1741 cgcgcgcctc ctgcatcgct gcgtaccagc cgccggcgac cgcgccgagc acgccggaca
     1801 gatgcagcga ttcggccgcc agataggacg catacggcgc cagcagcgtc aggccggtcg
     1861 agatcgattc atcggcccag ccacggtgcg acagcccgcg ccgcagccgc ccgagcaccc
     1921 aggtcgccgc cagcccggcg agcacgccgc cgacggcgac cagcatgaag ttcgccgccg
     1981 ccgcgccggc cgagaagctg ccggtcagcg ccgcggcgac cgagaagcgg aacgccacca
     2041 gccccgaggc atcgttgatc aggctttcgc cgttgaggag atgcgtcagc cgcgccggca
     2101 gccgcagccg ctcggtgatc gacgacaccg cgaccgcatc ggtcggcgag atcaccgccc
     2161 cgagcgcgaa gccggccgcg agcggcaccg acgggatcag ccagtgcacg aggtagccgg
     2221 tcgcgagcac cgtgcagagc acgagcccga gcgccagccc gaggatcgga tagcgcaggt
     2281 tcaggaactc gcgcttcggg atcagccagg catcggcgaa cagcagcggc ggcacgaaca
     2341 ccagaaagaa ggtctcggga tcgggcgcga ccgcgtgcat gcccggcacc agcgccagca
     2401 gcgtgccggc cgggatcagc agcagcggca gcggcatggc gctgcgccgc gacaccgcac
     2461 cgcaggcaag cacggcgaac agcagcacga ggatcgcgag cggcaggctc atcgaggcgg
     2521 gactccggac ggggggaaac ggtcgtcaag tgtcgtgtga ccgacgctca acgtcgagcc
     2581 gaggccgaag cgtcagcaat ttccgttctg catccggaca ggtgcaaaac aggcggcggc
     2641 cggttgccgt ctgcgtcacg cgctgcggag ttcagcccac cagcccgtgc aggagcccga
     2701 gccccgcaca gacgccgatc accggaatca cgccggcctt gtagcggatc agcgccacca
     2761 gcgcgccggc gccgatcacg gcggcgagcc attcgaaacg cccgccgaag ccggcgggcc
     2821 acagcacgtg ctcggcgaag aacagcgcca ggctcaggat cacgccgacc acggcggcgg
     2881 tgatgccggt cagcggggcc gtgaacttga gatccccgcg cgtcgcctcg acccaggggc
     2941 cgccgatcag gatgaacagg aaggacggca ggaacgtgaa cacggtcgcg accacggcgc
     3001 cgagcgcgcc ggcccaggcc agggcgtccg gcccgaggac ctccttggtc caggcaccga
     3061 cgtagccgac gaaggcgacg accatgatca gcggtcccgg cgtggtctcg ccgagcgcga
     3121 ggccgtcgat catctgcgcg ccggtgagcc agtggaagtg ctcgactgcg ccctgataga
     3181 cgtacggcag caccgcgtag gcaccgccga aggtcagcag cgccgccttc gtgaagaaca
     3241 ggcccatgtc gaccagcacg ccgccgaaaa cgagggccag cagcccgagt ccgccggccc
     3301 agagcccgaa gccaccgagc gcaaggcgcg tgagccgccc gcggtcgatg cgcgcgtgcg
     3361 ccggggtcgg cgtgtggtca tcgatcagcg ccgggccgta gctcgccttg ccggcgccgt
     3421 ggccgccccc gccgcggaag cactgcggcg ccagccgccc gccgagcgcc ccgatcaggc
     3481 cggcgccgat cacgatcagc ggaaacggca gatcgaacac ggcgatgccg aggaaggccg
     3541 cggcggcgat ggcccacagc acggggttct tcagcgcccg cgagccgatg cgccaggccg
     3601 cgaacacgac gatcgcggtg accgccggct tgatgcccca cagcacgccg gccacggccg
     3661 gcacctgacc gaaggccagg taaatccagc tcagtgcgat cagcacgaac accgagggca
     3721 gcacgaacag cgcgccggcg gcgatgccac ccttcgtacc gtgcagcagc cagccgatgt
     3781 aggtcgcgag ctgctgcgcc tcggggccgg gcaggaccat gcagtagttc agcgcgtgca
     3841 ggaagcgcga ttcgctgatc cagcgccggt gttcgaccag atcctgatgc aggatcgcga
     3901 tctgtccggc cggcccgccg aagctgatgc agccgaggcg cagccagtag cgcagcgcct
     3961 cgccgaagcc gggcgccggc ggtgctgtca gcgcatcgga ccggtccggc gccgggggca
     4021 tgaggggttc gggggcagtc atcgcgatac ctgcgggcag tctgggagaa gaacgattcc
     4081 ggcggccccc gaccgcggtg gcggcttccg acatgtcgtt ggcccgacag cttgccgaag
     4141 cggccacgga ctgcccagtg cgacacccgg cgccgcgctg ctgtccggtc cggaaccacg
     4201 actggcgggg tcgctccggg gggccgggaa gcccttaccc caaaatcctc tcccggaggt
     4261 atagcgcggc tgaatcgcgg gctccggcaa gacgccctca gaccagcgcg agcaccagcg
     4321 ccagcaccgg cgccgacagc agcgtcgaca gcacgatgct gcccgaggcg agcgcctcgt
     4381 cgccgccgag ctggcgcgcg agcacgtagg aagccgacgc ggtcggcgtc gacagcagga
     4441 tcagcgccga gcgcagctgc tccggcgcga gtccgagcag ccagccgaag ccgaagccga
     4501 gcagcggcag cagcgcggtc ttgatcagcg ccgccccgaa caccggccgc agccggctgc
     4561 cgatgtgcgc accgatcagc gagccgccga tgccgagcag cgccagcggc agcgccatgc
     4621 gcccgagcgc atcgaggctg cgctccagac tcggcggcag tccggccccg ctggccgcga
     4681 gcgcgaggcc gatgacgcag gcgagcagtt gcggattggt cgcgaggctg cgccagagcc
     4741 cggcgcggcc gacgccgggc gtgctgcggc tggcttcgag caccagcacc gcggcgatgc
     4801 tgtagagcag gatcaccggc gccgaggcga tcagcgcggc gctgcgcacc tccgggtggc
     4861 cgggcgaggc gtagacgatc accggcaggc tgatgaagta caggttgccg cgaaacgccg
     4921 cctgcacgaa ggtgccgcgc gcggcgcgcc cggccccgca ggccccggcg caataccagg
     4981 ccagcacgcc gccgatcacg gtcgcgacca gcacggtcgt gaacagcccg gccgccgcca
     5041 gcggctggaa cgggccggtg gcgatgtcgt gcacggtcag gcagggcagg ccgatccagt
     5101 aggtgagccg gttgaggccg gtcagcgtcg tcagcgacag gaagccgctg cgccgcagcg
     5161 cggcgccgag cgcgatcagc aggaagaccg gcgccagcga atcgaagatc ggccagaggc
     5221 tcatgacggg cgctcccggt acgacgcaag gcgctgcgcc ggccccgggc gcggc
//
LOCUS       DAOQIE010000272         5224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9015_length_5224_cov_6.482298, whole genome shotgun sequence.
ACCESSION   DAOQIE010000272 DAOQIE010000000
VERSION     DAOQIE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9015_length_5224_cov_6.482298"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK144_14705"
     CDS             <1..293
                     /locus_tag="PK144_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67024.1"
                     /translation="SGHAAACKAVDAGSIPTLASNYLILQFLTTQDSCRNPAIHRHRR
                     PGAQGFCIMCMMSGMPRVRPFVVPPAAPEIFLRAPAFARIGQPEDHTTALAG"
     gene            complement(290..727)
                     /locus_tag="PK144_14710"
     CDS             complement(290..727)
                     /locus_tag="PK144_14710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNF67025.1"
                     /translation="MARIAHAEFEELCREHVPFVGSLGIRVETLDAGRCVLRVPFRDD
                     FLRPGGTVSGPIQMALADIVMYGVVLSLLGRVDLAVTTNLTCNFLRRPKPVDLIGDGR
                     ILKLGKRLAVGEVLLYSDGETEPVAHVTCTYSIPPGAGQPIAN"
     gene            complement(730..1395)
                     /locus_tag="PK144_14715"
     CDS             complement(730..1395)
                     /locus_tag="PK144_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNF67026.1"
                     /translation="MTQPTDAPALPTLDWYFDYLSPFAYFQLERFTAEPPPGVVLRPQ
                     PVLLGALLGACGARGPAEIPLMRTLTYRHCLWQARRAGLPYRMPPAHPFNPLPALRLT
                     VALGAGPDTVRTVFRHLWAEGRTLEGEDWQALCARLGLSVAEAGALAASPAAKDGLRE
                     RTEAAIARGVFGVPCSIVHGELFWGNDTTGMLLDFLADPALFRDAEMQRVSTLPAAIQ
                     RRA"
     gene            complement(1505..3136)
                     /locus_tag="PK144_14720"
     CDS             complement(1505..3136)
                     /locus_tag="PK144_14720"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNF67027.1"
                     /translation="MPDRLKPALPFLYALCLCALLSLVVAGRAEAHAALLASDPLDGA
                     QLSLQPAAVELRFNEPVRPLMLRVLDRKGTTIASLAAGAPVTQSLRVTLPAGGDGVYL
                     ASWRVASADDHPVAGSLSWRVGTAGADTGAPVAIAAGAVSPPLVLRLLAELTLLIAAG
                     GALALPWLRPEGKARLRSVRVVRVAARLGLALLLPRLLAEGAALAGTSDWFEDETYAA
                     LAHTAYPLAAALALPGLLVLACLPKTGEWKRGWLLAGAVPALLSFGVGGHAVHALTGG
                     WLAPVLVVHAAAAAIWLGALWPTEYALRSVDPKIDDQRPALAVLGRFAHRGRELLGAL
                     AVSGLLIACVQLGPPSPAWGNTYARLFMAKLGLAFLLFALGALNRWCWLPALARGRRR
                     AASRLRLSVRLCRGLLVAVVALSVALGTTMPPRAGAGAVAPPPAALVERHWPSAYGPR
                     LGLNVAMQAGRPAATLSVTGPDDQPFTIREAVLVLERPEAGIEPLRRRLDERAPGRYG
                     NDDLLLPLAGPWQGYVEIWIDDFSLERQPLAFTLP"
     gene            complement(3169..3645)
                     /locus_tag="PK144_14725"
     CDS             complement(3169..3645)
                     /locus_tag="PK144_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HNF67028.1"
                     /translation="MSFGASAHVTLERLEAPAGSHYKAVLRVPHGCSGAATTGVRVRI
                     PDGVVSVKPQPKPGWTLETERGPLAEAYESHGQTIRDGVREVRWHGGVLPDEQFDEFA
                     ILMKLPAGAGRTLAFATVQDCIGGAVERWIGLPAAGQSAHDLPRPAPLLRLTDPVP"
     gene            4050..>5224
                     /locus_tag="PK144_14730"
     CDS             4050..>5224
                     /locus_tag="PK144_14730"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNF67029.1"
                     /translation="MRCPLPCLGLAAATLTLPVLAADGDTPLLEVIVTGRAPASLTVP
                     DIDAARAELATVPGGAGVVEAADEQRGRSSTLQDSLGFATGVFVQPRFGAEESRLSIR
                     GSGVQRTFHLRGIRLLQDGVPVNQADGGGDFQVLDPLALEYTEVWRGANALRFGAATL
                     GGAVNFISPTARSPAGAGSRLHFEAGSHGYLRPFASYGAADSQRDGYLAISGFAQEGF
                     REHAEQETWRLSGNWGERLGAGVENRVWIGAVNSNSELPGNLTKAQMRRDPTAAAAGN
                     VALDQQRNFDLFRIADQLTLVKDGARTDFAAYFVHKDLHHPIFQVLHQVSQDAGLSLI
                     HMLEAPLAGRANRLTAGVLASRGRLDDHRWVNLAGHTGNPTAHNEGTAWNLEAFVEN"
BASE COUNT          814 a         1933 c         1827 g          650 t
ORIGIN      
        1 agagtggtca tgcagcggcc tgcaaagccg tggacgccgg ttcgattccg accctagcct
       61 ccaactattt gatcctgcag tttttaacga ctcaggattc ctgccggaac cctgcgatcc
      121 accgtcatcg acggcccgga gcgcagggtt tctgcattat gtgcatgatg tccggcatgc
      181 cccgcgtacg gcctttcgtc gtcccgccgg ccgcgccgga gatattcctg cgcgcgccgg
      241 cattcgcgcg catcgggcag cctgaagatc acacgacagc gcttgcgggc taattcgcga
      301 tgggctgtcc ggcccccggc ggaatcgaat acgtgcaggt cacgtgtgcg accggttccg
      361 tctcgccgtc ggaatacagc agcacctcgc cgaccgccag gcgcttgccg agcttcagga
      421 tgcggccatc gccgatcagg tcgaccggct tcgggcggcg caggaagttg caggtcaggt
      481 tggtggtcac cgccagatcg acgcgtccga gcagcgacag cacgacgccg tacatgacga
      541 tgtcggcaag cgccatctgg atcggaccgg acacggtgcc gccggggcgc aggaagtcgt
      601 cgcgaaacgg cacgcgcagc acgcagcggc cggcgtccag ggtttcgacg cggatgccga
      661 gcgagccgac gaaggggacg tgctcgcggc agagttcctc gaattcggcg tgtgcgatgc
      721 gcgccatgct caggccctgc gctggatggc ggccggcagc gtgctgacgc gctgcatctc
      781 cgcatcacgg aacagcgccg gatcggccag gaagtcgagc agcatgccgg tggtgtcgtt
      841 gccccagaac agttcgccgt ggacgatcga gcacggcacg ccgaacacgc cgcgcgcgat
      901 cgccgcttcg gtgcgctcgc gcaggccgtc cttcgcggcc ggactcgcgg ccagcgcccc
      961 ggcctcggcc accgacagcc cgagccgcgc acacagcgcc tgccagtcct cgccctccag
     1021 cgtccggccc tcggcccaca gatgccggaa caccgtgcgc acggtgtcgg ggccggcacc
     1081 gagcgcgacc gtcagccgca gcgccggcag cggattgaag ggatgggccg gcggcatccg
     1141 gtacggcagg ccggcgcgac gcgcctgcca caggcagtgg cggtaggtca gcgtgcgcat
     1201 cagcggaatc tcggccgggc cgcgcgcgcc gcaggccccg agcaaggcgc cgagcagcac
     1261 cggctgcggc ctcaggacca cgcccggcgg cggttcggcc gtgaagcgtt cgagctggaa
     1321 gtacgcgaac ggcgacaggt aatcgaagta ccagtccagc gtcggcaagg caggggcatc
     1381 ggtcggctgc gtcatggcgg cggctcgcgg cgggcgggat cgggcgcatc gtcggacctc
     1441 ggtccgggcg ccggcaagcc gcaatacggc atggccggaa tgcgggcaac gcgcagtgcc
     1501 atgctcacgg cagcgtgaag gcgagcggct ggcgttcgag cgagaagtcg tcgatccaga
     1561 tttcgacgta accctgccag gggccggcga gcggcagcag caggtcgtcg ttgccgtacc
     1621 ggcccggcgc ccgctcgtcc agccgccggc gcagcggctc gatgccggct tcgggccgtt
     1681 cgagcaccag cacggcctcg cggatcgtga acggctgatc gtcggggccg gtgacgctca
     1741 gcgtcgccgc cggccggccg gcctgcatcg cgacgttcag cccgagccgg ggtccgtagg
     1801 cgctcggcca gtgccgctcg acgagggctg ccggcggcgg agccacggct ccggccccgg
     1861 cccgcggcgg catcgtcgtg ccgagcgcga cgctgagcgc gaccaccgcc accagcaggc
     1921 cgcggcagag gcgcacgctc agccgcagcc ggctcgccgc ccggcgccgg ccgcgcgcga
     1981 gcgccggcag ccagcaccag cggttcagcg cgccgagcgc gaacagcagg aaggcgagtc
     2041 cgagcttggc catgaacagc cgggcgtagg tgttgcccca ggccggcgac ggcggaccga
     2101 gctgcacgca ggcgatcagc aggccgctca cggccagtgc gccgagcagt tcgcgcccgc
     2161 gatgcgcgaa gcgcccgagc acggccagcg ccggccgctg gtcgtcgatc ttcgggtcaa
     2221 cgctgcgcag cgcatattcg gtcggccaca gcgcgccgag ccagatcgcc gcagccgccg
     2281 catgcacgac cagcaccggc gccagccagc cgccggtgag cgcgtgcacg gcatggccgc
     2341 cgacgccgaa gctcagcagc gccggcacgg caccggcgag cagccagccc cgcttccatt
     2401 cgccggtttt cggcaggcag gcgagcacca gcagcccggg cagcgccagc gccgcggcca
     2461 gcggataggc ggtgtgggcc agggctgcat aggtttcatc ctcgaaccag tcggaggtgc
     2521 cggccagcgc cgcgccttcg gcgagcagac gcggcagcag cagcgccagc ccgaggcgcg
     2581 ccgccacgcg cacgacacgc acgctgcgca gccgcgcctt gccttcgggc cgcaaccacg
     2641 gcagcgccag cgcgccgccg gcggcgatca gcaatgtcag ctcggccagc agccgcagca
     2701 ccagcggcgg cgataccgca cctgccgcga tcgccaccgg cgcaccggta tcggccccgg
     2761 ccgtgccgac ccgccagctc aggctgccgg cgaccggatg atcgtcggcc gaagccacgc
     2821 gccagctcgc gagatagacg ccatcgcccc cggccggcag cgtcacgcgc aggctctgcg
     2881 tgaccggggc gccggcggcg agactcgcaa tcgtcgtgcc cttgcgatcg agcacgcgca
     2941 gcatcagcgg ccgcaccggc tcgttgaagc gcagttcgac cgccgccggc tggagactca
     3001 gctgcgcccc gtcgagcgga tcgctggcga gcagcgccgc atgcgcttcg gcacgccccg
     3061 ccacgacgag gctcagcagg gcacaaagac acagggcata caggaacggc agggcgggtt
     3121 tcaggcgatc gggcatgcgc ttctggcagg aggcaaacgg aacatccgtc agggaacggg
     3181 atcggtcagg cgcagcagcg gcgccggccg gggcagatcg tgcgcggact ggccggcagc
     3241 aggcaacccg atccagcgtt cgacggctcc gccaatgcaa tcctgcacgg tcgcaaacgc
     3301 cagcgtgcgt cccgccccgg ccggcagttt catcaggatc gcgaattcgt cgaactgctc
     3361 atcgggcagg acgccgccat gccagcgtac ctcgcgcacg ccgtcgcgga tggtctggcc
     3421 gtggctctcg taggcttcgg cgagcggacc gcgttcggtc tccagcgtcc agcccggttt
     3481 cggctgcggc ttcaccgaca ccacgccgtc cgggatgcgc acgcgcacgc cggtggttgc
     3541 cgcgccgctg cagccgtgcg gcacgcgcag caccgccttg taatggctgc cggccggcgc
     3601 ttcgagtcgc tccagcgtca catgcgccga ggcaccgaag gacatcagcc atgccgcgca
     3661 gcttgccgcg caggccgatc gccatttcgc ctggcgctgc gggaggtcgg cacagaggat
     3721 acgggtggac gagggcttcg gcagggacat cggcgcgact ccggcagacg agggaaccgc
     3781 cattgtcggc atcgcccact gcccgccggc agcgcctgcg accatcggtc gcaggcgacg
     3841 gacggcaagg accaatcgcc ggcagccatg cggccggatt gcggacaagt agcggccggt
     3901 gcaagggttt tgcggcaccg cggattgtgc atcggccacc ccgcggcaag cctgcgcacg
     3961 cctcgcctgc gacaccatgt cacgcgacgg ctccccgcag gcggcacgag catgccgggc
     4021 ctccgccgct gcccgaacgg accctcccca tgcgctgccc cctgccctgc ctcggcctcg
     4081 ccgccgccac gctgaccctg cccgtgctgg ccgccgatgg cgacaccccg ctgctggagg
     4141 tcatcgtcac cggccgcgcc ccggcctcgc tgaccgtgcc cgacatcgac gccgcccgcg
     4201 ccgaactcgc caccgtgccc ggcggcgccg gcgtggtcga ggccgccgac gagcagcgcg
     4261 ggcgcagttc gacgctgcag gacagcctcg gcttcgcaac cggcgtgttc gtgcagccgc
     4321 gcttcggcgc cgaggaatcc cggctgtcga tccgcggctc cggcgtgcag cgcaccttcc
     4381 acctgcgcgg catccgcctg ctgcaggacg gcgtaccggt caaccaggcc gacggcggcg
     4441 gcgacttcca ggtgctcgac ccgctggcgc tcgaatacac cgaggtctgg cgcggcgcca
     4501 acgcactgcg cttcggtgcc gcgacgctcg gcggcgcggt caatttcatc agccccaccg
     4561 ctcgcagccc ggccggggcc ggcagccggc tgcacttcga ggccggcagc catgggtatc
     4621 tgcggccgtt cgcgagctac ggcgccgccg acagccagcg cgacggctat ctggcgattt
     4681 cgggcttcgc gcaggaaggc ttccgcgagc acgccgaaca ggaaacctgg cggctgtccg
     4741 gcaactgggg cgaacggctc ggtgccggcg tcgagaaccg cgtctggatc ggcgccgtca
     4801 acagcaactc ggaactgccg ggcaacctca ccaaagcgca gatgcgccgc gacccgacgg
     4861 ccgccgccgc cggcaacgtc gcgctcgacc agcagcgcaa cttcgacctg ttccgcatcg
     4921 ccgaccagct gacgctggtc aaggacggcg cgcgcaccga cttcgccgcg tatttcgtcc
     4981 acaaggacct gcaccacccg atcttccagg tgctgcatca ggtatcgcag gacgccggcc
     5041 tgagcctgat ccacatgctc gaagcgccac tcgccgggcg ggcgaaccgc ctgacggccg
     5101 gcgtgctggc gagccggggt cggctcgacg atcaccgctg ggtgaacctc gccggccata
     5161 ccggcaaccc gaccgcgcac aacgaaggca cggcctggaa tctcgaagcc ttcgtcgaga
     5221 acca
//
LOCUS       DAOQIE010000273         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9109_length_5190_cov_5.283739, whole genome shotgun sequence.
ACCESSION   DAOQIE010000273 DAOQIE010000000
VERSION     DAOQIE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9109_length_5190_cov_5.283739"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2535)
                     /locus_tag="PK144_14735"
     CDS             complement(<1..2535)
                     /locus_tag="PK144_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67030.1"
                     /translation="MTQGPTASRPTAAAAGDDELTRAVLHHQAGELAEAEALYRAALA
                     RQPDDAPVLNLLGQIRLERGDAQEAASLLGRAWQLAPQTPGLALVLGQALHAHGQLAA
                     ARDCLQQARQAAPADTGLLAELASVEQDDGHFAAAAALLRERLAQAPEDAHAAWHLAL
                     ALLSLGELDAGWAQYEARSRVFGVDYTGLGMQRWDGTPLDGDGEALLLLAEQGVGDEI
                     LFASCVPDALARAESVTLFCDPRLQPLFARSFPGASVLPLVRGGGVQRSVERPACTHY
                     LEAGSLPRWFRRRIEDFPAVPGYLLADAARAAHWRRWLAGLGKGLKIGLCWRSGLRGG
                     ARERHYAALEAMAPLFGVPGVRWVSVQYDPSAPELAAAQARFGVPIAEPPGLDQRNAL
                     DEVAALLDGLDLVISASTAVAELAAALGVPVWRFLTGSGWPMLGTDAMPWHPTQRLWS
                     RPPDQPDWQPTFTAMAQALAKVPPRERPVDAVASRQLDAALALFRKGRHAPAREAAVT
                     LTGQRPDLAGVWHLLGLAAREGGEGALAHAAFVRALTLTPQEPSLAYNFGALAERGGA
                     LDLAIHLYRRALRLAGDWPEARLALAETLTRRGGAALDANAAAAALPDFEAALALEPT
                     LADAWNNAGLALLRAGRPQEGCAAHDTAQQLAPADVRYRWNAAYTRLAAGDLAGGWAD
                     YRWRWRGIHVPNDYPFPEAAPGESLAGRHVLVFAEQGLGDEILFSTCLPDLLAEARAV
                     TLECDPRLVPLYARSFPGVRLIGSRRSGLDWERLAADIDVKLPLGELACRYRPRLDAF
                     PPQPALPLRAGADAVRHWQRRLQAVFDAGVTVGLAWRSGLRAANRTN"
     gene            complement(2480..2944)
                     /locus_tag="PK144_14740"
     CDS             complement(2480..2944)
                     /locus_tag="PK144_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67031.1"
                     /translation="MARIEASMTHIDPQMPCKEAQMTRGGPQMPCIEASMTHIDPPMV
                     RIEASMTHIDTGCAALASPLAAHPEPSARGDGAGAGGELRPRSGRRTANGPRGHPSIR
                     ESRGARNYSRCAAENPAISFLSAPRAGAPILRARDDPGTDRFPPHGCRCRRR"
     gene            complement(3086..4144)
                     /gene="pseI"
                     /locus_tag="PK144_14745"
     CDS             complement(3086..4144)
                     /gene="pseI"
                     /locus_tag="PK144_14745"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HNF67032.1"
                     /translation="MSCTGFEIAGRRIGPDAPPFVIAEMSGNHNQSLQRALAIVDAAA
                     AAGAHAIKLQTYTADTMTLDLDEGEFHIDDPASLWRGESLYRLYRQAYTPWEWHAPLF
                     ARAREHGLIAFSTPFDASAVDFLDSLDVPAWKVASFENTDLPLIRRMAATGRPMIIST
                     GMATVGELDETVRTARAAGCRDLVLLKCTSTYPATPADTHLRTLPHLRALFGCEVGLS
                     DHTMGLGVAVAAVALGASVIEKHFTLARADGGVDSAFSIEPHELAQLVTESERAWQAL
                     GAIHYGPTAREVPSLRHRRSLYAVRDIAAGEAFTADNVRAIRPGLGLPPKELERVLAS
                     RARRAIRRGEPLAYELLG"
     gene            complement(4171..4890)
                     /locus_tag="PK144_14750"
     CDS             complement(4171..4890)
                     /locus_tag="PK144_14750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF67033.1"
                     /translation="MPLMPAPPAPAVVLLDLDDTLYAERDWLLSGCRAVAARAEALLG
                     RSAAELYDWLLQRLLTEGRERLFDALLAAHGRCDPALSALLVHVFRSHRPRLTAFADV
                     APALAAWRARGCGLGLITDGVSCVQHAKLDGLGLRAGFDLVLCTDDLPGAARKPAPLA
                     FQLALAHFGCAPEQAVYIGDDPSKDFLAPRALGMRTLRIDRHLPWPLQSRRDFPDSHQ
                     AHRTIESLAEAAAPDASGLAA"
     gene            complement(4881..>5190)
                     /locus_tag="PK144_14755"
     CDS             complement(4881..>5190)
                     /locus_tag="PK144_14755"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF67034.1"
                     /translation="GVTVMDPALVELGAAVVRALGLIGHATVQCFHDAAGSRLIEVNP
                     RFGGGAALGFAAGADSPRWLLRLARGGSIEPQLGCQRDGLYLLRHSHDVFVAAEDVCR
                     "
BASE COUNT          736 a         1870 c         1935 g          649 t
ORIGIN      
        1 gttcgtgcgg ttcgccgcac gcaggccgct gcgccaggcc aggccgaccg tgacgccggc
       61 gtcgaacacc gcctgcaggc gccgctgcca gtggcgcacc gcatcggccc cggcgcgcag
      121 cggcagcgcc ggttgcggcg ggaaggcatc gagcctcggg cggtagcggc aggcgagttc
      181 gccgagcggc agcttcacgt cgatgtccgc agcgagccgc tcccagtcga gaccgctgcg
      241 ccggctgccg atcaggcgca cgccggggaa gctcctcgca tacagcggca ccagccgcgg
      301 gtcgcactcc agcgtcacgg cccgcgcctc ggcgagcaga tccggcagac aggtcgagaa
      361 caggatctcg tcgccgaggc cctgctcggc gaacaccagc acatgccgcc cggcgagtga
      421 ttcgcccggc gcggcttccg gaaacgggta gtcgttcggc acgtggatgc cgcgccagcg
      481 ccagcggtag tcggcccagc cgccggcgag atcaccggcg gcgaggcgtg tgtaagcggc
      541 gttccagcgg tagcggacat cggcgggtgc gagctgctgt gccgtgtcgt gcgccgcgca
      601 accctcctgc ggccggccgg cgcgcagcag ggccagcccg gcgttgttcc aggcatcggc
      661 gagcgtcggt tccagcgcca gcgcggcctc gaagtccggc agcgccgcgg cggccgcatt
      721 ggcatcgagg gccgcgccgc cgcggcgggt cagcgtctcg gccagcgcca gacgcgcctc
      781 cggccagtcg ccggccagtc gcagcgcccg gcggtacagg tggatcgcca gatcaagcgc
      841 gccgccgcgc tccgccagtg cgccgaagtt gtacgcgagg ctcggctcct gcggcgtcag
      901 cgtcagcgcg cgcacaaagg cggcgtgcgc cagcgccccc tccccgcctt cgcgcgccgc
      961 cagcccgagc agatgccaga cgccggcgag atcgggccgc tggcctgtca gcgtcaccgc
     1021 ggcctcgcgc gccggggcgt ggcggccttt gcgaaacagc gccagcgcgg cgtcgagctg
     1081 acggctggcg acggcatcca ccgggcgctc gcggggcggc accttcgcca gcgcctgcgc
     1141 catcgccgtg aaggtcggct gccagtcggg ctgatcgggc gggcgcgacc acagccgctg
     1201 cgtcggatgc cagggcatcg catcggtgcc gagcatcggc cagccgctgc cggtcaggaa
     1261 gcgccagacc ggcacgccga gcgcggcggc gagttcggcg accgcggtgc tcgccgagat
     1321 caccagatcg aggccatcga gcagggccgc gacctcgtcg agcgcgttgc gctggtcaag
     1381 ccccggcggc tcggcgatcg ggacgccgaa gcgcgcctgc gcggccgcca gttccggcgc
     1441 ggacgggtcg tactgcacgc tgacccagcg cacgcccggc accccgaaca gcggcgccat
     1501 ggcttcgagc gcggcgtaat ggcgttcgcg ggccccgccg cgcaggccgc tcctccagca
     1561 gagaccgatc ttcaggccct tgccgagccc ggcgagccag cgtcgccagt gcgcggcacg
     1621 cgcggcatcg gcgagcagat agccgggcac ggccgggaag tcctcgatcc ggcggcggaa
     1681 ccaccgcggc aggctgccgg cttcgaggta atgcgtgcag gccggacgct cgacgctgcg
     1741 ctgcacgccg ccaccgcgca cgagcggcag cacgctcgcg cccgggaacg agcgcgcgaa
     1801 cagcggctgc aggcgcggat cgcagaacag tgtcacggat tcggcgcgcg ccagcgcatc
     1861 gggcacacag gaagcgaaca ggatctcgtc gccgacgccc tgctcggcca gcagcagcag
     1921 tgcctcgccg tcgccatcga gcggcgtgcc gtcccagcgc tgcatgccga ggccggtgta
     1981 atcgacgccg aacacgcgac tgcgcgcctc gtactgcgcc cagccggcgt cgagctcgcc
     2041 gaggctgagc agcgccagcg ccagatgcca ggccgcgtgc gcatcctcgg gcgcctgcgc
     2101 gaggcgctcg cgcagcagcg cggcagcggc ggcaaagtga ccgtcgtcct gctcgaccga
     2161 ggccagttcg gcgagcagcc cggtatcggc cggcgccgcc tgccgcgcct gctgcaggca
     2221 gtcacgcgcc gccgcgagct gcccgtgcgc atgcagtgcc tgcccaagca ccagcgccag
     2281 ccccggcgtc tgcggcgcga gctgccaggc ccggccgagc agcgaggccg cctcctgcgc
     2341 atcaccacgc tccagccgga tctgcccgag caggttcagc accggcgcat cgtcgggctg
     2401 gcgcgcgagg gcggcgcggt acaaggcttc ggcctcggcc agctcgccgg cctggtgatg
     2461 cagcacggcg cgcgtcagtt catcgtcgcc ggcagcggca gccgtggggc gggaagcggt
     2521 cggtccctgg gtcatcgcgg gctcgcagga tcggtgcgcc ggcgcgcggc gcagacagga
     2581 aggaaatggc cgggttttca gcggcgcacc gactgtagtt gcgggcaccc cggctttctc
     2641 ggatcgacgg atggccgcga ggcccgttcg ccgtgcgccg gccggaccgc ggccgcagct
     2701 cgcctccggc ccctgcgcca tcgccccgcg ccgacggctc ggggtgcgct gcgagcggcg
     2761 aagccaaggc ggcacacccc gtatcgatgt gcgtcatcga tgcttcgatg cgcaccatcg
     2821 gcggatcgat gtgcgtcatg gacgcttcga tgcacggcat ctgcggaccc ccgcgcgtca
     2881 tctgcgcttc cttgcacggc atctgcggat cgatgtgcgt catggatgct tcgatgcgcg
     2941 ccatctgcac ttccctgcgc ggcaggcgtg atcctctgcc cggcatgggt tcgccgctgt
     3001 cgcacaacga aacccgcagc ggcgagtccc ggtcgtcgat gccgcgggat cagcccacgt
     3061 ccggggcgtg gcggcagcgc gtggttcagc caagcagctc gtaggcgagc ggttcgccgc
     3121 ggcggatcgc gcggcgcgcg cggcttgcga gcacgcgttc gagctccttc ggcggcaggc
     3181 cgaggccggg gcggatcgcg cgcacgttgt cggccgtgaa ggcttcgccg gcggcgatgt
     3241 cgcgcaccgc gtacagcgag cggcggtggc gcagcgacgg cacttcgcgg gcggtcgggc
     3301 cgtagtggat ggcaccgagc gcctgccagg cgcgttccga ttcggtgacg agttgcgcaa
     3361 gttcgtgcgg ctcgatcgag aacgccgaat cgacgccgcc atcggcacgc gccagcgtga
     3421 agtgcttctc gatgacgctg gccccgagcg cgaccgcggc gaccgcaacc ccgagcccca
     3481 tcgtgtgatc cgacagcccg acctcgcagc cgaacagcgc gcgcaggtgc gggagcgtcc
     3541 gcaggtgggt gtcggccggc gttgccggat aggtgctcgt gcacttgagc agcacgaggt
     3601 cccggcagcc ggcggcgcgc gccgtgcgta ccgtctcgtc gagttcgccg accgtcgcca
     3661 tgccggtcga gatgatcatc ggcctgccgg tggcggccat gcgccggatc agcggcaggt
     3721 cggtgttctc gaaggacgcg accttccagg ccggcacgtc gagactgtcg aggaaatcga
     3781 cggcactcgc atcgaagggc gtgctgaagg cgatcagccc gtgctcgcgg gcacgcgcga
     3841 acagcggcgc gtgccattcc cagggcgtgt aggcctgacg gtagagccgg tacagcgact
     3901 cgccgcgcca gagactcgcc gggtcatcga tgtggaactc gccctcgtcg aggtcgagcg
     3961 tcatcgtgtc ggcggtatag gtctgcagct tgatggcgtg cgcaccggcg gcggccgcgg
     4021 catcgacgat ggcgagcgcg cgctgcagcg actggttgtg gttgccggac atctcggcga
     4081 tcacgaacgg cggtgcgtcc ggaccgatcc ggcggccggc gatttcaaag cctgtgcaac
     4141 tcatgatgac gttctccagc acgggcggca tcaggcggcg agtccggacg catccggtgc
     4201 cgccgcttcc gccagcgatt cgatggtgcg gtgtgcctgg tgactgtccg gaaagtcgcg
     4261 gcgcgactgc agcggccagg gcaggtgacg gtcgatgcgc agcgtgcgca tgccgagcgc
     4321 gcgcggcgcg aggaaatcct tggacggatc gtcgccgatg tagacggcct gctccggcgc
     4381 gcagccgaag tgcgcgagcg cgagctggaa ggcgagcggc gcgggcttgc gcgctgcccc
     4441 gggcaggtcg tcggtgcaga gcaccagatc gaagccggcg cgcaggccga ggccgtcgag
     4501 cttggcgtgc tgcacgcagc tgacgccgtc ggtgatcagc ccgagcccgc agccgcgcgc
     4561 acgccaggcc gccagcgccg gcgccacgtc ggcaaaggcc gtcagccgcg gccggtgact
     4621 gcgaaacaca tgcacgagca gcgcactgag cgccgggtcg cagcgcccgt gcgcggcgag
     4681 cagcgcatcg aacagacgct cgcggccctc ggtcagcagc cgctgcagca gccagtcgta
     4741 gagttcggcg gccgaacgcc cgagcagcgc ttcggcgcgc gcggccaccg cgcggcagcc
     4801 gctcagcagc cagtcgcgct cggcgtacag cgtgtcgtcg agatcgagca gcacgacggc
     4861 gggcgcaggt ggcgcgggca tcagcggcat acgtcctccg ccgccacgaa cacgtcgtgg
     4921 ctgtgacgca gcaggtacag gccgtcgcgc tggcagccga gctgcggctc gatgctgccg
     4981 ccgcgcgcca gacgcagcag ccagcgcggc gagtcggcgc cggccgcgaa gccgagcgcg
     5041 gccccgccgc cgaagcgcgg gttgacctcg atcagccggc tgccggcagc gtcgtggaag
     5101 cactgcacgg tggcgtggcc gatcaggccg agtgcacgca cgacggccgc gccgagttcg
     5161 accagcgccg ggtccatgac cgtgacgccg
//
LOCUS       DAOQIE010000274         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9169_length_5167_cov_8.816315, whole genome shotgun sequence.
ACCESSION   DAOQIE010000274 DAOQIE010000000
VERSION     DAOQIE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9169_length_5167_cov_8.816315"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PK144_14760"
     CDS             <1..453
                     /locus_tag="PK144_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNF67035.1"
                     /translation="HRLKGTAGNLGLLALAAAAVEAEQALRAGVAPPEACAPLAGALD
                     EALTAIREYAPPKPPAQDEAAPGTAATPGEAGHELAALLDRMLAALDTDEPDAIEPLL
                     DELAACVPAPQIAELRTAIENFDFRRAEAAAHALAPRLGLSGVIPESS"
     gene            487..1578
                     /locus_tag="PK144_14765"
     CDS             487..1578
                     /locus_tag="PK144_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF67036.1"
                     /translation="MIHGPILIVDDEPLNLAVMREVLGGLYRLAFARSGEEALRAAVR
                     HQPLLILLDVRMPDMDGYEVCRQLKADAQTAAIPVIFVSGCTDTLDETRGFEAGAVDY
                     LVKPVSPPIVRARVRAHLSLVSLERLERSYHDAIYMLGEAGHYRDNDTGVHIWRMAAY
                     AAALGGACGLDAGICRRLELAAPMHDTGKLGIPDAILRKPGPLDADEWVIMRTHPRIG
                     HDILARSQAPVFQMAAQIALRHHEKWDGSGYPDGLCGTGIPETARIVAIADVFDALTM
                     KRPYKEAWPAERALAELHKGAGRHFEPRLVTAFADILPRILEIKAQWDEREAAGHATD
                     LPTLPADYAPAPPGNAADAHAAGSDARPA"
     gene            complement(1634..2068)
                     /gene="fur"
                     /locus_tag="PK144_14770"
     CDS             complement(1634..2068)
                     /gene="fur"
                     /locus_tag="PK144_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNF67037.1"
                     /translation="MGSKELKQVGLKVTLPRMKILAILQEHGGAQHLSAEDIYKSLIA
                     SGEEVGLATVYRVLTQFETAGLVKRHHFEGGQSVFELNQGEHHDHILCLQCGRIEEFV
                     DEVIETRQREIAAQHGFRLAEHSLILYGNCTNPDCPHRPPTH"
     gene            2155..2511
                     /locus_tag="PK144_14775"
     CDS             2155..2511
                     /locus_tag="PK144_14775"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNF67038.1"
                     /translation="MPRIPACLGLAALLVCGSGCSYFSDFSMPLPSFYKTKVRQGNYL
                     DEATLGTITPGMSKREVQQRLGTPLVTDPFHQNRWDYVYSYRPGTYDDGEPEQRRVTL
                     YFERDVLSRVEYPPQG"
     gene            complement(2533..2847)
                     /locus_tag="PK144_14780"
     CDS             complement(2533..2847)
                     /locus_tag="PK144_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018949765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNF67039.1"
                     /translation="MNVEVAYARPDQQLILALELPHGASVAEALAAASGPLQERFPEL
                     DLAAAEVGVFGKAVTRSQPLQPGDRVEIYRKLVADPKEVRKQRAAEGRLKKQRREDAA
                     GD"
     gene            complement(2852..3289)
                     /locus_tag="PK144_14785"
     CDS             complement(2852..3289)
                     /locus_tag="PK144_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HNF67040.1"
                     /translation="MATIKKSALVAYSAARMYALVADIEAYPQFLPWCRSTRILARNG
                     DELRASIEMAKGAVNKSFSTCNRLQANKMIEMRLIDGPFRHLEGYWRFEGLGEDACRV
                     SLDLEFEFSSTLLRLAIGPIFNQIANTLVDSFCKRAVQIYGRG"
     gene            3426..3899
                     /gene="smpB"
                     /locus_tag="PK144_14790"
     CDS             3426..3899
                     /gene="smpB"
                     /locus_tag="PK144_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNF67041.1"
                     /translation="MSKKSKNAPPSTIAQNKKAFHDFFIEERLEAGLALEGWEAKALR
                     AGKVQLKESYVLLKDGESWLFGCHISPLLSASTHVHPDATRTRKLLLHREQILQLVIA
                     VERRGYTLIPLSLYWKKGRAKLEIGVARGKKDYDKRATEKERDWEREKGRLMRHQ"
     gene            complement(3945..5006)
                     /locus_tag="PK144_14795"
     CDS             complement(3945..5006)
                     /locus_tag="PK144_14795"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNF67042.1"
                     /translation="MATREEVLWNCRSALAAAVGAENPDDAVVEALAGLLLGHNAGLT
                     LGEVVAAGVAAGYPPGALLKALLRCQLFDLEATLSDLDADNKPADERMDALRALFAQY
                     QDVQNRFIESFSEHYEHEAADARQRAERERDAELLAQLRADWRRDGQVELFNYFRELQ
                     VPTTVPLDEIDGNYVAVARTSRLAIVLAAGERGRVAFAPTRDNGRVALLELQSAKDDI
                     LLFRIAGLRPHPQGQRRRLRVEVREDAPFSMRLPDGRQALAYVRDFSVIGMGLECADA
                     SKLEPGTTLQCSGRLGPRLRKVDLDLTVTVRWRVESTQGMRVGVELSMEPGTREVLDK
                     LVREYEQEAIKALNLIAEG"
BASE COUNT          810 a         1874 c         1720 g          763 t
ORIGIN      
        1 caccggctca agggcacggc cggcaacctc ggcctgcttg cgctcgccgc cgccgccgtc
       61 gaggccgaac aggccctgcg cgccggcgtg gcgccgcctg aggcctgcgc accgctcgcc
      121 ggcgccctcg acgaggccct gaccgcgatc cgcgaatacg caccgcccaa gccaccggcg
      181 caagacgaag ccgcccccgg cacggccgcc acgcccggcg aggccggcca cgagctggcc
      241 gcgctgctcg accggatgct ggcagcactc gataccgacg agccggacgc gatcgagccg
      301 ctgctcgatg agcttgccgc gtgcgtgccg gcgccgcaga tcgcggaact gcgcaccgcg
      361 atcgagaact tcgatttccg ccgcgccgag gcggctgccc atgccctcgc gccacgactc
      421 gggctgtcgg gcgtgatacc ggaaagttcc tgaaaaatcc ccccagagaa cccgagggaa
      481 cccgaaatga tccatggacc gatcctgatc gtcgacgatg aaccgctgaa cctcgccgtg
      541 atgcgcgagg tgctcggcgg cctgtaccgg ctcgcgtttg cccgcagcgg cgaggaagcc
      601 ctgcgcgccg ccgtgcgcca ccagccgctg ctgatcctgc tcgacgtgcg catgccggac
      661 atggacggct acgaggtctg ccggcagctg aaggccgatg cgcagaccgc cgccatcccg
      721 gtgatcttcg tcagcggctg caccgacacg ctcgacgaga cccgcggctt cgaggccggt
      781 gccgtcgact acctcgtcaa gcccgtgtca ccaccgatcg tgcgtgcgcg cgtgcgggcg
      841 catctgtcgc tggtcagcct ggagcggctg gagcgcagct accacgatgc gatctacatg
      901 ctcggcgagg ccggccatta ccgcgacaac gacaccggcg tgcacatctg gcgcatggcg
      961 gcatatgccg cggccttggg cggcgcctgc gggctcgacg ccgggatctg ccggcggctc
     1021 gaactcgccg caccgatgca cgacaccggc aagctcggca tcccggacgc gatcctgcgc
     1081 aagcccggcc cgctcgacgc ggacgaatgg gtgatcatgc gcacccaccc gcgcatcggc
     1141 cacgacatcc tcgcgcgcag tcaggcgccg gtgttccaga tggccgcgca gatcgcgctg
     1201 cgccatcacg agaaatggga cggcagcggc taccccgacg gtctctgcgg caccggcatc
     1261 cccgagaccg cgcgtatcgt cgcgatcgcc gatgtcttcg atgcgctgac gatgaagcgc
     1321 ccgtacaagg aagcctggcc ggccgaacgg gcgctggcgg agctgcacaa gggcgccggc
     1381 cgccacttcg aaccgcggct ggtgacggcc tttgccgaca tcctgccgcg catcctggag
     1441 atcaaggcgc agtgggacga gcgcgaagcc gccggtcacg cgaccgacct gccgacgctg
     1501 cccgcggact acgcgccggc cccgcccggt aacgctgccg atgcgcacgc cgcaggcagc
     1561 gacgcacgcc cggcctgagc aggccgcagc agcctcggga cggagagcga acgggaggga
     1621 gcgggcgcgc gtttcagtgg gtcggcggcc ggtgcgggca gtcggggttc gtgcagttgc
     1681 cgtacaggat cagcgagtgc tcggcgagcc ggaagccgtg ctgcgcggcg atctcgcgct
     1741 ggcgcgtctc gatgacctca tcgacgaact cctcgatgcg gccgcactgc aggcacagga
     1801 tgtgatcgtg gtgctcgccc tgattcagct cgaacaccga ctggccgccc tcgaagtgat
     1861 ggcgcttgac caggccggcg gtctcgaact gcgtcagcac gcggtagacg gtcgccagac
     1921 cgacttcctc gccgctcgcg atcagggact tgtagatgtc ttcggcgctc aggtgctgcg
     1981 cgccgccgtg ttcctgcagg atcgcgagga tcttcatgcg cggcagcgtg accttcaggc
     2041 ccacctgctt cagttctttc gagcccaagg gccggttctc ctcgcgcgcc gtcagccgga
     2101 ccggcggaat cggttatgat gcccgagtct aagtcaccgt gcccgccgcc tcaaatgccc
     2161 cggattcccg cctgcctcgg cctcgccgca ctgctcgtct gcggcagcgg ctgctcgtat
     2221 ttctcggatt tctcgatgcc gctgccctcg ttctacaaga ccaaggtccg gcaggggaat
     2281 tacctcgacg aggcgacact cggcacgatc acgcccggca tgagcaagcg cgaggtgcag
     2341 cagcgcctcg gcacgccgct ggtcaccgat ccgttccacc agaaccgctg ggactacgtc
     2401 tacagctacc gtcccggcac ctacgacgac ggcgagcccg agcagcggcg cgtgacgctg
     2461 tacttcgagc gcgacgtgct gagccgcgtc gaatacccgc cgcagggctg agccgggctt
     2521 gcggccggac gctcagtcgc cggccgcgtc ctcacgccgc tgcttcttca gccggccctc
     2581 ggcagcgcgc tgcttgcgga cttccttggg gtcggcgacg agtttgcggt agatctcgac
     2641 ccggtcgccc ggctgcagcg gctggctgcg cgtgacggcc ttgccgaaca cgccgacctc
     2701 ggccgcggcc aggtcgagtt ccggaaaacg ctcctgcagc ggcccgctcg ccgccgccag
     2761 cgcctcggcg acgctggccc cgtgcggcag ctcgagcgcc agaatcagtt gctggtccgg
     2821 ccgcgcgtag gcgacctcca cgttcatctt ttcatccccg gccatagatc tgtaccgccc
     2881 gcttgcagaa ggaatcgacc agcgtgttcg cgatctggtt gaagatcggc ccgatcgcga
     2941 gccgcagcaa ggtgctggag aactcgaact cgagatccag cgacacccga catgcatcct
     3001 cccccagccc ctcgaagcgc cagtagcctt ccagatgccg gaacggcccg tcgatcaggc
     3061 gcatctcgat catcttgttg gcctgcaggc gattgcaggt cgagaacgac ttgttgaccg
     3121 cgcccttggc catctcgatg ctcgcgcgca gttcgtcgcc gttgcgcgca aggatgcgtg
     3181 tcgagcggca ccacggcagg aactgcggat aagcctcgat gtccgcgacg agcgcgtaca
     3241 tgcgcgccgc cgaataggcg accagcgcgc tcttcttgat cgtcgccacc cgggggctcc
     3301 ctgatgtgtt ctcgtgaatg tccaccggtc cggtgccggc gcggcgcgca ttctctggca
     3361 attgccgcgc ccgctcaacc gcgccgccgg tccgcagcca gcacggcgcc gggtacaatc
     3421 cgccgatgag caagaaatcc aagaacgccc cgccatcgac catcgcccag aacaagaagg
     3481 ccttccatga cttcttcatc gaggaacgcc tggaggccgg cctcgccctc gagggctggg
     3541 aggccaaggc gctgcgcgcc ggcaaggtgc agctgaagga gagctacgtg ctgctgaagg
     3601 acggcgagag ctggctgttc ggctgccaca tctcgccgct gctgtcggcc tcgacgcacg
     3661 tgcacccgga cgccacgcgc acgcgcaagc tgctgctgca ccgcgagcag atcctgcagc
     3721 tcgtcatcgc tgtcgagcgg cgcggctaca cgctgattcc gctgtcgctg tactggaaga
     3781 agggccgcgc caagctggag atcggcgtcg cgcgcggcaa gaaggactac gacaagcgcg
     3841 cgaccgagaa ggagcgcgac tgggagcgcg agaagggccg gctcatgcgc caccagtaag
     3901 cgggcgcggg cgacggcgcg ctgcgcaagg catgcggcgc cgcttcagcc ctcggcgatc
     3961 aggttcagcg ccttgatcgc ctcctgctcg tactcgcgca ccagcttgtc gagcacctcg
     4021 cgcgtgcccg gctccatcga aagctcgacg ccgacacgca tgccctgcgt actctcgacg
     4081 cgccagcgca cggtcacggt caggtcgaga tcgaccttgc gcagccgcgg cccgagccgg
     4141 ccgctgcact gcagcgtcgt gcccggctcc agcttcgagg cgtccgcgca ttcgagcccc
     4201 atgccgatca ccgagaagtc gcgcacgtag gcgagcgcct gacggccgtc gggcaggcgc
     4261 atgctgaacg gcgcgtcctc gcgcacctcg acgcgcaggc gccggcgctg gccttgcggg
     4321 tgcgggcgca ggccggcgat gcgaaacagc aggatgtcgt ccttggccga ctgcagttcg
     4381 agcagcgcga cgcggccgtt gtcgcgggtc ggcgcaaagg cgacgcgccc gcgctcgccg
     4441 gcggcgagca cgatcgccag ccgcgaggtg cgcgcgaccg cgacgtaatt gccgtcgatc
     4501 tcgtcgagcg gcaccgtcgt cggcacctgc agctcgcgga aatagttgaa cagctcgacc
     4561 tgcccgtcgc gacgccagtc cgcacgcagc tgcgcgagca gctcggcgtc gcgctcgcgc
     4621 tcggcgcgct gccgcgcatc ggcggcctcg tgctcgtagt gctcgctgaa gctctcgatg
     4681 aagcggttct gcacgtcctg gtactgcgcg aacagcgcgc gcagcgcatc catgcgctcg
     4741 tcggccggct tgttgtcggc gtcgaggtcg ctgagcgtgg cttccaggtc gaacagctgg
     4801 cagcgcagca gcgccttcag cagcgcgccg ggcggataac cggccgcgac accggccgcg
     4861 accacctcgc cgagcgtcag cccggcgttg tggccgagca gcagcccggc cagagcctcg
     4921 acgacggcat catccggatt ctcggcaccg accgccgcag ccagcgccga acggcagttc
     4981 cagagcactt cctcgcgcgt cgccaccttg ccgctgcctc ctgttgctgc gcaggaccga
     5041 tccggtcatc cggcccgcgt ggttgatggg cgccgtctcg cggctcactc cggccgcatg
     5101 tgcgggaaca ggatgacctc gcggatcgac ggctgatcgg cgaacagcat caccagccgg
     5161 tcgatgc
//
LOCUS       DAOQIE010000275         5163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9184_length_5163_cov_5.523297, whole genome shotgun sequence.
ACCESSION   DAOQIE010000275 DAOQIE010000000
VERSION     DAOQIE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_5163_cov_5.523297"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..5121
                     /locus_tag="PK144_14800"
     CDS             256..5121
                     /locus_tag="PK144_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNF67043.1"
                     /translation="MDDQREGPMNDHRRFPIVALGASAGGLGALQAFFRALPVDRRDG
                     MACFIVAQHLSPRHRSQIAAILGQCTAQTCLDLVDGLQPEPGFVYVLPPGRGIAVCED
                     GSFRLTEQAGSPTPTPSIDGLFETLARHYGARTVGIVLSGTGSDGSRGARAIADAGGS
                     VFVESETSAGYPQMPIATRDTGVTSCQGTPGTLALRVYALAISRFEGTTPEDADPAVA
                     ASLLSLLGSLADLRGIEANARTVDLLHRAAEAAGCRTLVDYRLHLLDDGAERLHLARR
                     LAALQRLWPIGAAQRAALLRHLAARAQTMQVGETELRLWVPACGRGESAYQLGILLAG
                     LQHPDGRPVSFRLFATDSDAEVLNQARLARYSRELLETCAGLTAADIDHCFIGDGELL
                     RPQPWLRERIVFSPHGVLNAPPFPRLDLVSLQLDNRSLTGAAAQLRVFHYALREGGLL
                     ALQGPRCEPDSQRFEVLDPAASLWRRRSGSGRLPQPPAFLGLDTGINPGLRELNAVLL
                     DALAPDALVLDAGGRCLQILGGAARYLDLRSPRPLTVPRALREPWRSALHRLLRELDL
                     GEEGAPGRQTSVIDGSGGPGVLRAVHLQLRPLPPSATGVDLRLLVFQPAAGPRQAPEG
                     SGSSDDGYENLLQALGEVNAELQSAVEELSTANEELEASGEELQASNEELEVANSELD
                     ARNREIAAVNEELAAILDASELAILVLDEGLCLRRFSPAAGIAFGIEEPNLGQLIVHA
                     GNFSRTGTALEAVESAQRTGLRTEFEVDIDARTWLFRVRRINVGSLLTPGWMLIAGTD
                     ITRLRSATEALRDSEEALRRTLESAPHGMAILQPDGGFLSVNPALCRLFGRPAEQLTT
                     LRFFDVLDPADHATMRPVFDALLAGEHSDANCELRGLLPDGTRIWLLLTAALVRDAAG
                     APLHCIVQFVDIHRRKRDESRLAALGTRLALALDAARLGIWNWNCVPDIVDGDARYAR
                     LFGIGQGPKSLDELLASVSIEARDRLRRAMTLACDHGVPFELELSTVHPERHLLVNGL
                     RERTGDGSHVVVGTVADVSELHATLAALREASARASAAAAVKSEFLANMSHEIRTPMN
                     AIIGLSDLALGSDDMELRTDYLRRIHVAGAALLTLLNDVLDFSKLEAGKLLLESIDFE
                     PLRVLDEVAELLAGAAHARGLRFELTPAAGLPARVHGDPTRLRQVLVNLLGNAIKFTE
                     HGHVGISLALAADGDALECVVSDSGIGIDETAVEQLFEPFTQADPSTTRRYGGTGLGL
                     SISRRLVAAAGGVLGAQRRAGGGSEFRFTWPVAPVPVPPGADAAQPPLPPGLVLLVAQ
                     DTFADLGDPLPVLDRLRVPVTAMALAQLSAAARRAGPALMPVLAWPHLDTDGRRAAVI
                     DWLAAHPELAARTLLVVARGEPSEPAPSLRRQVARVLAHPPLALLLDQTTLSAVSPQL
                     PLAGYRVAVVEDNPVNQLVVTQYLERAGAEVSVHDQGGELLDWLAQRVSGEGPQLILM
                     DVQMQGMDGIEATRRLRADARWCGLPVVALTAHAFADEIERCLAAGMNAHLSKPIEPS
                     KLIEAVLHHALPPPGRPGRPACPWPVRAGRRPPASAARRRRPTGRRRCPDGWQRWRNR
                     PREVSRLDCGR"
BASE COUNT          664 a         1908 c         1862 g          729 t
ORIGIN      
        1 tcatcgacag cctgcagatc ggcgacctgc gcgtcagcag cgtcgatgcc gtcgtgctcg
       61 acggggacta tccgcgcagc ccgctgctcg gcatgaacgt gctgcagcgc ctgacgatgc
      121 gcaccgaggg ggatctgctg atcctgcagc agatgcaacc atagatgagg tgcgcttgcc
      181 gggatcgtgc aggaccgccg gccccgtccg gggctgccgg agccggcctg ccgcaggcca
      241 cccggggagg ctgccgtgga tgaccagcgc gagggaccga tgaacgatca ccggcgcttt
      301 ccgatcgtcg cactcggggc ctcggccggc gggctcgggg cgctgcaggc cttcttccgg
      361 gcgctgccgg tcgaccggcg cgacggcatg gcctgcttca tcgtcgcgca gcatctctcg
      421 ccgcgtcatc gcagccagat cgccgcgatc ctcggccagt gcacggcgca gacctgtctc
      481 gatctggtcg acggcctgca gccggagccc ggcttcgtct acgtgctgcc gccgggacgg
      541 gggatcgcgg tctgcgagga cggcagcttc cggctgaccg agcaggccgg cagccccacg
      601 ccgacgccga gcatcgacgg gctgttcgag accctcgccc gccactacgg cgcccgcacg
      661 gtcggcatcg tgctctccgg caccggttcg gacggctcgc gcggggcgcg ggccatcgcc
      721 gatgccggcg gcagcgtgtt cgtcgagagc gagaccagcg ccggttatcc gcagatgccg
      781 atcgcaaccc gcgacaccgg cgtcaccagc tgccagggca cgccgggcac gctggcgctg
      841 cgcgtctacg cgctggcgat cagccgcttc gagggcacga cgccggagga tgccgacccg
      901 gcggtggccg cgtcgctgct gtcgctgctc ggttcgctcg ccgacctgcg cggcatcgag
      961 gccaacgccc gcacggtcga tctgctgcac cgggcggccg aggccgccgg ctgccggacg
     1021 ctggtcgact accgcctgca cctgctcgat gacggcgccg agcgcctgca cctcgcccgg
     1081 cgcctggcgg cgctgcagcg gctgtggccg atcggggccg cgcagcgcgc ggcgctgctg
     1141 cgccatctcg ccgcgcgcgc gcagacgatg caggtcggcg agaccgagct gcgcctctgg
     1201 gtgccggcct gcgggcgcgg cgagtcggcc taccagctcg gcatcctgct cgccggcctg
     1261 cagcaccccg atggccggcc ggtgagcttc cggctgttcg cgaccgacag cgatgccgag
     1321 gtgctgaatc aggcacgtct ggcgcgctat tcgcgcgagc tgctggaaac ctgcgccggg
     1381 ctgacggcgg ccgacatcga tcactgcttc atcggcgacg gcgagctgct gcgcccgcag
     1441 ccctggctgc gcgagcgcat cgtgttcagt ccgcacggcg tgctgaacgc gccgccgttc
     1501 ccgcgcctcg acctcgtgag cctgcagctc gacaaccgca gcctcaccgg agccgccgcg
     1561 cagctgcgcg tgttccatta cgcgctgcgc gagggcggac tgctggcgct gcaggggccg
     1621 cgctgcgaac ccgactcgca gcgcttcgag gtgctcgatc cggccgccag cctgtggcgg
     1681 cggcgctccg gcagcggccg gctgccgcag ccgccggcct tcctcggcct cgacacgggc
     1741 atcaaccccg gcctgcgcga actgaacgcc gtgctgctcg atgcgctcgc gcccgatgcg
     1801 ctggtgctcg atgccggcgg tcgctgcctg cagatcctcg gcggtgcggc gcgctatctc
     1861 gacctgcgct cgccgcggcc gctgactgtg ccgcgcgcgc tgcgcgagcc ctggcgcagc
     1921 gcgctgcacc ggctgctgcg cgagctcgac ctcggcgagg agggcgcacc cggtcgccag
     1981 acctcggtga tcgacggcag cggcggaccg ggggtgctgc gggccgtgca cctgcagctg
     2041 cgcccgctgc cgccgagcgc gaccggcgtc gacctgcgcc tgctggtgtt ccagcccgcg
     2101 gccggtccgc ggcaggcccc cgagggctcg ggcagcagcg atgatggcta cgagaacctg
     2161 ctgcaggccc tcggcgaggt caacgccgag ctgcagtcgg cggtcgagga gctcagcacc
     2221 gccaacgagg aacttgaggc gagcggcgag gaactgcagg ccagcaacga ggagctggag
     2281 gtcgccaact ccgagctcga cgcccgcaac cgcgagatcg ccgccgtcaa cgaggaactg
     2341 gcggcgatcc tcgatgccag cgaactcgcg atcctcgtgc tcgacgaggg cctctgcctg
     2401 cgccgcttca gcccggccgc cggcatcgcc ttcggcatcg aggaaccgaa cctcggccag
     2461 ctgatcgtgc atgccggcaa cttcagccgc accggcacgg cgctggaggc ggtcgagagc
     2521 gcgcagcgca ccgggctgcg caccgagttc gaggtcgaca tcgatgcgcg cacctggctg
     2581 ttccgcgtgc gccgcatcaa cgtcggcagc ctgctgacgc cgggctggat gctgatcgcc
     2641 ggcaccgaca tcacgcggct gcgcagcgcc accgaggcgc tgcgcgacag cgaggaagcg
     2701 ctgcggcgca cgctggagag cgcgccgcac ggcatggcca tcctgcagcc cgacggcggc
     2761 ttcctgagcg tcaacccggc gctctgccgg ctgttcggcc gcccggccga acagctgacc
     2821 acgctgcgct tcttcgacgt gctcgatccg gccgatcacg cgacgatgcg cccggtgttc
     2881 gatgcgctgc tcgccggcga gcacagcgac gcgaactgcg agctgcgcgg cctgctgccc
     2941 gatggcaccc gcatctggct gctgctgacc gcggcgctgg tgcgcgacgc cgccggcgcg
     3001 ccgctgcact gcatcgtgca gttcgtcgac atccaccggc gcaagcgcga cgaaagccgg
     3061 ctcgccgcgc tcggcacgcg gctcgcgctc gcgctcgatg ccgcgcgcct gggcatctgg
     3121 aactggaact gcgtgccgga catcgtcgat ggcgatgcgc gctacgcgcg gctgttcggc
     3181 atcggtcagg gtccgaagtc gctcgatgag ctgctcgcca gcgtctcgat cgaggcccgc
     3241 gaccgcctgc gccgggcgat gacgctcgcc tgcgaccacg gcgtgccctt cgagctggag
     3301 ctgtcgaccg tgcaccccga gcgccacctg ctggtcaacg gcctgcgtga gcgcaccggt
     3361 gacggcagcc acgttgtcgt cggcaccgtc gccgatgtca gcgagctgca cgcgacgctt
     3421 gcggcgctgc gcgaggcgag cgcgcgcgcc agcgccgcgg ctgcggtcaa gtccgagttc
     3481 ctcgccaaca tgagccacga gatccgcacg ccgatgaacg cgatcatcgg cctgtccgat
     3541 ctcgcgctcg gttccgacga catggaactg cgcaccgatt acctgcgccg catccacgtc
     3601 gccggcgccg cgctgctgac gctgctgaac gacgtgctcg acttctcgaa gctcgaagcc
     3661 ggcaagctgc tgctcgaatc gatcgatttc gaaccgctgc gcgtgctcga cgaggtcgcc
     3721 gagctgctgg ccggcgctgc gcatgcacgc ggcctgcgct tcgaactgac gccggctgcc
     3781 ggcctgccgg cgcgcgtgca cggggacccg acgcggctgc gccaggtgct ggtcaacctg
     3841 ctcggcaacg ccatcaagtt caccgagcac ggccacgtcg gcatctcgct ggcgctggct
     3901 gcggatggcg atgcgctcga atgcgtcgtc agcgacagcg gcatcggcat cgacgagacg
     3961 gccgtcgagc agctgttcga gccgttcacg caggccgatc cgtcgaccac gcgccgctac
     4021 ggcggcaccg gcctcgggct gtcgatcagc cggcggctgg tcgcggcggc cggcggcgtg
     4081 ctcggcgcgc agcggcgcgc cggtggcggc agcgagttcc gcttcacgtg gccggtcgcg
     4141 ccggtgccgg tcccgcccgg ggccgatgcc gcgcagccgc cgctgccgcc ggggctggtg
     4201 ctgctggtcg cgcaggacac cttcgccgat ctcggcgatc cgctgccggt gctggaccgc
     4261 ctgcgcgtgc cggtcaccgc catggcgctt gcgcagcttt cggccgccgc ccggcgcgcc
     4321 gggccggcgc tgatgccggt gctggcctgg ccgcacctcg ataccgatgg ccggcgtgcg
     4381 gccgtcatcg actggctggc cgcgcacccg gagctggcgg cgcgcacgct gctggtggtc
     4441 gcccgcggcg agccctccga accggcgccg tcgctgcgcc ggcaggtcgc gcgcgtgctc
     4501 gcgcatccgc cgctggcgct gctgctcgac cagaccacgc tgagcgccgt ctcgccgcag
     4561 ctgccgctcg ccggctaccg ggtggcggtg gtcgaggaca acccggtcaa ccagctcgtg
     4621 gtcacgcagt atctggagcg ggccggggcc gaggtctcgg tccacgacca gggtggcgag
     4681 ctgctcgact ggctcgcgca gcgtgtgagc ggggagggtc cgcaactgat cctgatggac
     4741 gtgcagatgc agggcatgga cggcatcgag gcgacgcggc ggctgcgcgc cgatgcgcgc
     4801 tggtgcgggc tgccggtcgt ggcgctgacc gcgcatgcct ttgccgacga gatcgagcgc
     4861 tgcctcgcgg ccggcatgaa cgcgcatctg tccaagccga tcgagccttc gaagctgatc
     4921 gaggcggtgc tgcaccacgc gctgccgccg cccggccggc cgggacgacc tgcgtgccca
     4981 tggcccgttc gagcaggccg acgtccgcct gcatcggccg cacggaggcg acggcccaca
     5041 ggtcgaaggc gttgcccgga cggatggcaa cggtggcgaa accggccgcg agaagtttcg
     5101 cgcctagact gcgggcggtg aatcctgtcc ggtgtgccat gtaggtccgg ccccgttcga
     5161 tcc
//
LOCUS       DAOQIE010000276         5131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9266_length_5131_cov_7.540189, whole genome shotgun sequence.
ACCESSION   DAOQIE010000276 DAOQIE010000000
VERSION     DAOQIE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_5131_cov_7.540189"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2619
                     /locus_tag="PK144_14805"
     CDS             <1..2619
                     /locus_tag="PK144_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF67044.1"
                     /translation="HRLWSEAERSADRLTERLSATLAGDVARERRLLALVVGLWLLFV
                     VGGALLLLAAIRKDRASRLRLLASEAGQQALLAALGDGVFVVQGGCFVWANPALLTLL
                     GYAQVEDFVGRDPAQVIAPEFLALWHPREHGAEEGLPALPGRCELRLLRRTPADAESG
                     GGDGEVWVELHAVVAPFAGRPALIGVARDIGARRAALSAALARTGSFEPLPVAVDADA
                     GADAHYLRRELYAQVQSNPAIFDFLQAGSLDGLWYWDLERPEHEWMNARFWRVLGYDP
                     ATKQHLAAEWQDLIFPEDLALAIDNFRRHCADARHPYDQIVRYRHADGSTVWVRCRGI
                     AIRDAAGRALRMLGAHTDVTGIMRAEEARSAAVALAESERRLRELADSLPQLVWTCRP
                     DGWCDFLSRRWVEYTGQPEAAQLGSGWLEHVYPDDRERLVAAWTAAVAGGHDFSVEFR
                     IRRAADGAWRWFDTRALPLRDAQGRIVRWFGTNTDVQAARETAEALRASEARFRALLD
                     QAADAIYVHDADGRLCDVNHRACETLGLPRATLLTMNVADVDPALPAAGLAFWHTLTP
                     GESLTLRSRHRRGDGSDFPVELSVGAVEIGGERRLLALARDISERERAEAEIHELNAS
                     LEQRVAERTAELTAANRELDAFAYAVSHDLRAPLRAMSGFSTALLEDCAGQLDADGRA
                     YLDQIIAGSRRMGELIDGLLALSRSTRGELRRERVDVTAIMARVREELEADEPQRRVH
                     WQVEPDLHVWGDAGMLDAVFANLAGNAWKYTARTADPVIRVQAAEAAAGFLCVCVSDN
                     GAGFDPRHAGKLFLPFQRLHRQDEFPGLGIGLATAQRIVHRHGGTIAASAAPGLGARF
                     CITLPGPQVASEEPES"
     gene            2616..3044
                     /locus_tag="PK144_14810"
     CDS             2616..3044
                     /locus_tag="PK144_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF67045.1"
                     /translation="MKTLLIVEDNPQDELLIRRALRKAALANPVDVVRDGQQALDWLW
                     REGEFAGHPDTGLPAVVLLDLSLPRVSGLDVLERLRADARTRLQPIVVLTSSDEERDR
                     LTSYAHGANAFVRKPLDFAEFAEAVARIGVFWLMTNVGPA"
     gene            complement(3151..3693)
                     /locus_tag="PK144_14815"
     CDS             complement(3151..3693)
                     /locus_tag="PK144_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNF67046.1"
                     /translation="MPIRSFENLVPHIAASAWIDDSAVVIGDVQIGAEASIWPMCVVR
                     GDIQSIRIGARSNVQDGTVIHVTHDSRFCPGGRATTVGEDVTIGHQALLHACTIGDRC
                     LIGMGAIVMDGAGIGAGAMLGAGCVVPPGKQLAGGFLYVGSPARQQRPLSEREIEFLG
                     YSAENYVKLAARHRAGSRSV"
     gene            3879..>5131
                     /locus_tag="PK144_14820"
     CDS             3879..>5131
                     /locus_tag="PK144_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNF67047.1"
                     /translation="MERSPRKVRKLLVANRSEIAIRAMRAAAELGIATVAIYSEEDRF
                     ALHRFKADESYRVGQGRKPIQAYLDIDDILRVAKEAGADAIHPGYGFLSENPDFAEAC
                     AQNGILFIGPSPEVMRTLGNKVAARNAAVAAGVPVMPATGPLPRDLDEVKKMAAAIGF
                     PFMLKASWGGGGRGMRVIETEADLGPQIEVARREAAAAFGNDEVYLEKLVRRARHVEV
                     QILGDRHGNIVHLFERDCTVQRRNQKVVERAPAPYLDEATRSELCESALRLARQVGYT
                     AAGTVEFLMDADTGRFYFIEVNPRIQVEHTVTEQVTGVDLIKAQIRVCEGAAIGTPAS
                     GVPAQADIRLHGHALQCRVTTEDPENGFTPDYGRIQAYRSAAGFGIRLDAGTAYSGAV
                     ITPYYDSLLVKVTAWSQDSDDAIRR"
BASE COUNT          753 a         1843 c         1775 g          760 t
ORIGIN      
        1 caccgcctgt ggtccgaggc cgaacgcagt gccgaccggc tgaccgaacg gctgtcggcg
       61 acgctcgccg gcgatgtcgc ccgcgaacgc cggctgctcg cgctcgtcgt cgggctgtgg
      121 ctgctgttcg tcgtcggcgg tgcgctgctg ctgctggcgg cgatccgcaa ggaccgcgcc
      181 agccgcctgc gcctgctcgc cagcgaggcc ggccagcagg cgctgctcgc cgcgctcggc
      241 gatggcgtgt tcgtcgtgca gggcgggtgc ttcgtgtggg cgaatccggc gctgctcacg
      301 ctgctcggtt atgcgcaggt cgaggacttc gtcgggcgcg accctgcgca ggtgatcgcg
      361 ccggagttcc tggcgctgtg gcacccgcgc gagcacggcg ccgaggaagg gctgccggcc
      421 ctgcccgggc gctgcgaact gcggctgctg cggcgcacgc ccgcagacgc ggagagcggg
      481 ggcggggacg gcgaggtctg ggtcgagctg catgcggtgg tcgcgccctt cgccggccgc
      541 ccggcgctga tcggcgtggc ccgcgacatc ggcgcgcggc gcgcggcgct gagcgcggcg
      601 ctggcgcgca ccggcagctt cgagccgctg ccggtcgcag ttgatgccga tgccggggcc
      661 gatgcgcact acctgcgccg cgagctgtac gcgcaggtgc agagcaaccc ggcgatcttc
      721 gacttcctgc aggccggctc gctcgatggc ctctggtact gggacctcga acggcccgaa
      781 catgagtgga tgaacgcgcg cttctggcgc gtgctcggat acgatccggc gacgaagcag
      841 catctggccg ccgagtggca ggacctgatc tttcccgagg acctcgcgct cgcgatcgac
      901 aacttccgca ggcactgcgc cgatgcccgg cacccctacg accagattgt ccgctaccgc
      961 catgccgacg gctcgacggt ctgggtgcgc tgtcgcggca ttgccatccg cgacgccgcc
     1021 ggccgggcgc tgcgcatgct cggggcgcac accgacgtga ccggcatcat gcgtgccgag
     1081 gaagcgcgca gcgccgccgt ggcgctcgcc gagagcgagc ggcggctgcg cgaactcgcc
     1141 gattcgctgc cgcagctggt ctggacctgc cgcccggatg gctggtgcga cttcctgagc
     1201 cggcgctggg tcgaatacac cggccagccc gaggccgcgc agctcggcag cggctggctc
     1261 gaacacgtct acccggatga ccgcgagcgg ctggtggcgg cgtggacggc ggcggtcgcc
     1321 ggcggccacg atttcagcgt cgaattccgc atccgccgcg ccgccgacgg cgcctggcgc
     1381 tggttcgaca cgcgcgcgct gccgctgcgc gatgcgcagg gccgcatcgt gcgctggttc
     1441 ggcaccaaca ccgacgtgca ggccgcgcgc gagaccgccg aggcgctgcg cgcgagcgag
     1501 gcgcgcttcc gggcgctgct cgatcaggcc gccgatgcca tctacgtgca cgatgccgac
     1561 gggcgcctgt gcgacgtgaa ccaccgtgcc tgcgagaccc tcggcctgcc gcgtgcgacg
     1621 ctgctgacga tgaacgtcgc cgatgtcgat ccggccttgc cggccgcggg tctggcgttc
     1681 tggcacacgc tcacgcccgg cgagtccctg acgctgcgca gccgccaccg ccgcggtgac
     1741 ggcagtgatt ttccggtcga gctcagcgtc ggcgccgtcg agatcggcgg cgagcgccgg
     1801 ctgctcgcgc tcgcgcgcga catcagcgag cgcgagcgcg ccgaggccga gattcacgag
     1861 ctgaacgcct cgctggagca gcgcgtcgcc gagcgcaccg ccgagctgac cgccgccaac
     1921 cgcgaactcg acgccttcgc ctacgccgtc tcgcatgacc tgcgcgcgcc gctgcgcgcg
     1981 atgtcgggct tcagcacggc gctgctggag gactgcgccg ggcagctcga tgccgacggc
     2041 cgcgcgtatc tcgaccagat catcgcgggc agccggcgca tgggcgagct gatcgacggc
     2101 ctgctggcgc tgtcgcgcag cacgcgcggc gagctgcgcc gcgagcgcgt cgatgtgacc
     2161 gcgatcatgg cgcgcgtgcg cgaggaactc gaagcggacg aaccgcagcg gcgcgtgcac
     2221 tggcaggtcg agccggacct gcacgtctgg ggcgatgccg gcatgctcga tgcggtgttc
     2281 gcgaacctcg ccggcaacgc ctggaagtac accgcccgca ccgccgatcc cgtcatccgc
     2341 gtgcaggcgg cagaggccgc tgccggcttc ctgtgcgtgt gcgtcagcga caacggcgcc
     2401 ggcttcgacc cgcgtcacgc cggcaagctg ttcctgccgt tccagcgcct gcatcgccag
     2461 gacgagtttc ccggcctcgg catcggcctt gcaaccgcgc agcgcatcgt gcaccgccac
     2521 ggcggcacga tcgccgcaag cgccgcgccc ggtctcggcg cccgtttctg catcacgctg
     2581 cccggtccgc aggtggcctc cgaagagccc gaatcatgaa gaccttgctg atcgtcgaag
     2641 acaacccgca ggacgaactg ctgatccgcc gcgccctgcg caaggccgcg ctcgccaatc
     2701 ccgtcgatgt ggtgcgcgac ggccagcagg cgctcgactg gctgtggcgc gaaggcgagt
     2761 tcgccggcca ccccgacacc ggcctgccgg ccgtcgtgct gctcgacctg agcctgccgc
     2821 gcgtcagcgg cctcgacgtg ctcgaacgcc tgcgcgccga tgcgcgcacg cgcctgcagc
     2881 cgatcgtggt cctgacctcc tcggacgagg agcgtgaccg cctcaccagc tacgcccatg
     2941 gcgccaacgc cttcgtgcgc aagcccctcg acttcgccga gttcgccgag gcggtcgcgc
     3001 gcatcggcgt gttctggctg atgaccaacg tcggcccggc ctgaaggcgc gccgcagcgc
     3061 gcagcggcat ccgggcgcat cgccgcgtgc ggcgcacggg aaaaaggcga ccgtccgctc
     3121 aatgccttgc aagggttttt ccgcaggcgc tcacacgctc cggctgccgg cccggtgccg
     3181 ggccgcgagc ttcacgtagt tttcggccga atagccgagg aactcgatct cgcgctcgct
     3241 gagcgggcgc tgctgccgcg ccgggctgcc gacgtacagg aagccgcctg cgagctgctt
     3301 gcccggcggc accacgcagc cggcgccgag catcgcgccg gcgccgatcc cggcgccgtc
     3361 catgacgatc gcccccatgc cgatcaggca gcgatcacca atcgtgcagg cgtgcagcag
     3421 cgcctgatgg ccgatggtca cgtcctcgcc cacggtcgtg gcgcgccctc cgggacagaa
     3481 acgcgaatcg tgcgtgacgt ggatgacggt cccgtcctgc acgttgctgc gcgcgccgat
     3541 gcggatcgac tggatgtcgc cgcgcaccac gcacatcggc cagatcgagg cttcggcgcc
     3601 gatctgcacg tcgccgatga cgacggcgct gtcgtcgatc caggcgctgg cggcgatatg
     3661 cgggacgaga ttttcgaaac tgcggatcgg catcgggcaa ttctccgggg caggcagtcg
     3721 gaatacgaac attattttgc actatgaaag tgcatgtgcg gaaaatcgca caatgcgcat
     3781 tgtcgtatgt gtggaattcc gctaatgtgg atagacaatg gtctgaattt caaccattaa
     3841 cactgcctat tcatgtgatt gtcgtagagg tagacaggat ggagcgcagt cctcgcaaag
     3901 tccgcaagct gctggtggcc aaccgcagcg agatcgccat ccgtgcgatg cgtgccgccg
     3961 ccgagctcgg catcgccacc gtcgcgatct attcggagga agaccgtttc gcgctgcacc
     4021 gcttcaaggc cgacgagagt taccgcgtcg gtcagggccg aaaacccatc caggcctatc
     4081 tcgacatcga cgacatcctg cgcgtcgcca aagaggccgg tgccgacgcc atccatcccg
     4141 gctacggctt cctgtccgag aaccccgact tcgccgaagc ctgcgcgcag aacggcatcc
     4201 tgttcatcgg tccctcgccc gaggtcatgc gcacgctcgg caacaaggtc gccgcgcgca
     4261 acgccgcggt cgccgccggc gtgccggtga tgccggccac cggcccgctg ccgcgcgatc
     4321 tcgacgaagt gaagaagatg gctgccgcca tcggcttccc gttcatgctc aaggcgagct
     4381 ggggcggcgg cggtcgcggc atgcgcgtga tcgagaccga ggccgatctc ggtccgcaga
     4441 tcgaggtcgc ccggcgcgag gccgccgcgg ccttcggcaa tgacgaggtc tatctcgaaa
     4501 agctcgtgcg ccgcgcgcgc cacgtcgagg tgcagatcct cggcgaccgg cacggcaaca
     4561 tcgtccacct gttcgagcgc gactgcacgg tgcagcggcg caatcagaag gtggtcgagc
     4621 gcgccccggc gccgtatctc gacgaggcca cgcgcagcga actctgcgaa tcggcgctgc
     4681 gcctcgcgcg ccaggtcggc tacacggccg ccggcaccgt cgaattcctg atggatgccg
     4741 acaccggcag gttctacttc atcgaggtca acccgcgcat ccaggtcgag cacaccgtca
     4801 ccgagcaggt gaccggcgtc gacctgatca aggcgcagat ccgcgtctgc gagggtgcgg
     4861 cgatcggcac gccggcgagc ggcgtgccgg cgcaggccga catccgcctg cacggtcatg
     4921 cgctgcagtg ccgcgtgacc accgaggacc cggagaacgg cttcacgccc gactacggcc
     4981 gcatccaggc ctaccgcagc gccgccggct tcggcatccg cctcgacgcc ggcaccgcgt
     5041 actcgggcgc cgtgatcacg ccgtattacg actcgctgct cgtgaaggtc acggcttggt
     5101 cgcaggattc cgacgacgcc atccggcgca t
//
LOCUS       DAOQIE010000277         5067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9435_length_5067_cov_7.180766, whole genome shotgun sequence.
ACCESSION   DAOQIE010000277 DAOQIE010000000
VERSION     DAOQIE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9435_length_5067_cov_7.180766"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..784)
                     /gene="modB"
                     /locus_tag="PK144_14825"
     CDS             complement(119..784)
                     /gene="modB"
                     /locus_tag="PK144_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNF67048.1"
                     /translation="MDGADWTPLWLSLKVAGFATLFAVLLGVAIALPFARRRFFGRDT
                     LDAILTLPMVMPPTVLGYYLIVAFGRQSWLGAWLEEQLGLRLMFTWQGAVLAATIVAF
                     PLVFKSARAAFEGVERTLENAARTLGDTEARVFFRVTLPVAMRGIVAGTLLAFARALG
                     EFGATLMIAGNLPGRTQTLSMAVYDAVQAGDQARANALVLLISVVCIAVLVTSARLLT
                     PRY"
     gene            complement(799..1551)
                     /gene="modA"
                     /locus_tag="PK144_14830"
     CDS             complement(799..1551)
                     /gene="modA"
                     /locus_tag="PK144_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF67049.1"
                     /translation="MRRIPPKAAAALLLAATLPATAAELTVSVAASMTNAFTELGKAY
                     EAAHPDTKLVFNFAASGPLLAQIVQGAPVDLFVSADAETMDKAGAGGHIDPGSRTSIA
                     GNALVLIVPLESRQAPKSLADLGSPAVQRIALGNPASVPNGRYAKAALEAAGLWETLQ
                     PKYVYAENVRQSLSYVTRAEVEAGFVFATDAALEAGKVRVAFPVPVAKPILYPMAIVA
                     GSKEKDAAAAFLRYVQSPDGQAVFARYGFTKP"
     gene            1940..2359
                     /locus_tag="PK144_14835"
     CDS             1940..2359
                     /locus_tag="PK144_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNF67050.1"
                     /translation="MKLSARNQYTGTVSEVRSGPVSTEVTIAINDADTLKATLTSESA
                     ADLGLAAGKPCVALFKASAVVLGTGEPGRLSARNVFTGTVSAVKTGAVSSEVTLTTTG
                     GLSIAATTTQEATSELGLVVGAPAFALVKASAVIVGV"
     gene            complement(2453..2932)
                     /locus_tag="PK144_14840"
     CDS             complement(2453..2932)
                     /locus_tag="PK144_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNF67051.1"
                     /translation="MYAIQTQGEFRHTEELSIGNRLPGRIVEIVRGPVMSEIELDTQA
                     GMVTAAITTRSLQRMRLAEGQDVLALIKATELAIEKPAAAEALLASSFRNQLEARVVA
                     LEAGTVLTAVELDTVVGPLTAVITARSARRLKLAPGDQVIGVVKATEVAIAREGRRR"
     gene            complement(2947..3474)
                     /locus_tag="PK144_14845"
     CDS             complement(2947..3474)
                     /locus_tag="PK144_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNF67052.1"
                     /translation="MTSVMPAAADVRVPALRLDGRVLRPLRLDAGALQGLVQHTVVRR
                     ALSCAARSLDPAVGLRCWQGVRLTALLELAGLQLDRQRERGGVCVHAHGRDGYVASFS
                     WSELFNGPAGGDVLVALGVDGMPLPAGDSPRLIAAHDWQSSARLVRGLCRIEVAGPVA
                     LRPDPGTAMTMTSRR"
     gene            3711..4529
                     /locus_tag="PK144_14850"
     CDS             3711..4529
                     /locus_tag="PK144_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNF67053.1"
                     /translation="MSRSPADSMQLEGRFWLDRGGKPFLGPGRVALLERIDACRSIAE
                     AARAMGMGYKTAWDLVDAMNQLAEQPLVVRVKGGRQGGGTELTDYGRSVVATFRALER
                     DYVAMLETLGARYPGFGAFDAHPRPPRLRTSARNQWSGHVTGIERHGFGALVTVDIGA
                     GAPVQAALSMNAVEELGLTDGSEVHVLVKATAIGIGVVAGWGGSGGNRLHGVIEHLQL
                     DAGGERAELTLAIAASRHVLAIIDPAMIAALGLQAGVAAWAQFEPAAVILAVGD"
     gene            4624..>5067
                     /locus_tag="PK144_14855"
     CDS             4624..>5067
                     /locus_tag="PK144_14855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF67054.1"
                     /translation="MSTACTLPVTQCDCIVIGAGASGLLVARLLARAGLKLVVLERGA
                     IGRESSWAGGGILSPLYPWRYDPAVNALAGWSQRAFAALAAGLHAQTGIDPEWTPNGM
                     LYLGGLETEAARAWAVAQAASLEALTPPDARGIEPVVPVHPAVREI"
BASE COUNT          757 a         1853 c         1728 g          729 t
ORIGIN      
        1 tcgaagcgca cgttcagcag gaattcgcgc cgccgcgcct gcaggcgaac gtcgatgtcg
       61 atgtccaggg tcgggcagcc ggcggcggac atggcggact ccttcatcgg aaagactttc
      121 agtaacgcgg cgtcagcagg cgcgccgacg tgacgaggac tgcgatgcag accacggaaa
      181 tcagcagcac cagcgcgttg gcgcgggcct ggtcgccggc ctgcacggcg tcgtagaccg
      241 ccatcgacag cgtctgcgtg cgccccggca ggttgccggc gatcatcagc gtcgcgccga
      301 actcgccgag ggcgcgcgcg aacgcgagca gcgtgccggc gacgatgccg cgcatcgcca
      361 ccggcagcgt gacgcggaag aacacgcgcg cctcggtgtc gccgagcgtg cgcgcggcgt
      421 tctccagcgt gcgctcgacg ccctcgaagg ccgcgcgcgc actcttgaac accagcggga
      481 aggcgacgat ggtcgcggcc agcaccgcgc cctgccacgt gaacatcagg cgcaggccga
      541 gctgctcctc cagccaggcg ccgagccagc tctggcggcc gaaggcgacg atcaggtagt
      601 aaccgagcac ggtcggcggc atgaccatcg gcagcgtcag gatcgcgtcg agcgtgtcgc
      661 gcccgaagaa gcgccggcgc gcgaacggca gcgcgatcgc aacgccgagc agcaccgcga
      721 acagcgtcgc gaagcccgcg accttcagcg acagccacag cggcgtccag tcagccccgt
      781 ccatcaccgt gcgcgccctc agggtttcgt gaagccgtag cgcgcgaaca cggcctggcc
      841 gtccggcgac tgcacgtacc gcaggaaggc cgctgccgcg tccttctcct tgctgccggc
      901 gacgatcgcc atcgggtaga ggatgggctt ggcgaccggc accgggaacg cgacgcgcac
      961 cttgccggcc tcgagcgcgg catcggtcgc gaacacgaag ccggcctcga cctcggcgcg
     1021 ggtcacgtag ctcaggctct ggcggacgtt ctcggcgtag acgtacttcg gctgcagcgt
     1081 ctcccagagc ccggcggcct cgagcgcggc cttcgcgtag cggccgttcg gcaccgaggc
     1141 cggattgccg agcgcgatgc gctgcacggc ggggctgccg agatcggcga gcgacttcgg
     1201 tgcctgcctc gactcgagcg gcacgatcag caccagcgca ttgccggcga tgctggtgcg
     1261 cgagcccggg tcgatgtgtc cgccggcccc ggccttgtcc atggtctcgg catcggccga
     1321 cacgaacagg tcgaccggcg cgccctgcac gatctgcgcg agcagcggcc cggacgccgc
     1381 gaagttgaag acgagcttcg tgtccggatg cgcggcctca taagccttgc cgagttcggt
     1441 gaaggcattg gtcatgctcg ccgcgaccga gacggtcagc tcggcggccg tcgccggcag
     1501 cgtcgcggcg agcagcaggg cagcggcagc cttcggggga atacgcctca tcatcacgct
     1561 ccagtcgggg ttccggcctg tcggaaaggc ttcattgcgc cgcagcccgt gtcgggttcg
     1621 tcggcattgc gacacgaagc cggccggcag cccggcggcg gcagacccgg ttgcgacgat
     1681 aaacgccatg ctccgaaatt tcgatatcct gttggatata tcgatggcga aacgatggct
     1741 gcatcacggc ctctgccacc ggctgcgccg gtgtccggtc atctccaagc aatcggcatg
     1801 ccttttctgg atcgtccgat ggaactggca cgctgcctgc ccggtgatcc gggcgcctgc
     1861 atcctgccgt cctgcacgac ggatacgcag ccacggcgcc gatcgatgcc gcttccccaa
     1921 cccagcagga gaacccgcga tgaagctcag cgcccgcaac cagtacaccg gcaccgtctc
     1981 cgaagtccgc agcggcccgg tcagcaccga agtcacgatc gcgatcaacg acgccgacac
     2041 gctgaaggcg acgctgacca gcgaatccgc cgctgacctc ggcctcgccg ccggcaagcc
     2101 ctgtgtggcg ctgttcaagg ccagcgcggt cgtgctcggc accggcgagc cgggccggct
     2161 cagtgcccgc aacgtgttca ccggcacggt ctcggccgtg aagaccggcg cggtcagcag
     2221 cgaggtgacg ctgaccacga ccggcggcct gagcatcgcc gcgaccacca cgcaggaagc
     2281 cacgtccgag ctcggcctcg tggtcggcgc gccggcgttt gcgctggtca aggccagcgc
     2341 ggtgatcgtc ggcgtctgat ccaccgcctg ccggcctgtt tccggacctg cgccgccgcg
     2401 ccggcgcagg tccggcgcag cagggcctgc gaagcgactg cgaagccggc gactagcgcc
     2461 gccggccctc gcgggcgatc gccacctcgg tcgccttgac cacgccgatc acctgatccc
     2521 ccggggcgag cttcagccgc cgggcgctgc gcgccgtgat caccgcggtc agcgggccga
     2581 cgacggtgtc gagctcgacc gccgtcagca cggtgccggc ctcgagcgcg acgacgcgcg
     2641 cttcgagctg gttgcggaag ctgctcgcga gcagcgcctc ggccgccgcg ggcttctcga
     2701 tcgccagctc cgtggccttg atcagcgcca gcacgtcctg gccctcggcc agacgcatgc
     2761 gctgcaggct gcgggtcgtg atcgccgccg tgaccatgcc ggcctgggtg tcgagctcga
     2821 tctccgacat caccgggccg cgcacgatct cgacgatgcg cccgggcagg cggttgccga
     2881 tgctgagttc ctcggtgtgc cggaactctc cctgtgtctg aatcgcgtac atgtcccctg
     2941 ctcccctcat cttctgcttg tcatggtcat cgcggtgccc ggatccggtc gcagcgcgac
     3001 cggtccggca acctcgatgc ggcagagtcc gcgcaccagc cgggcgctgc tctgccagtc
     3061 gtgtgcggca atgagccgcg gtgaatcccc ggccggcagc ggcatgccgt cgacgccgag
     3121 cgccaccagc acgtccccgc cggccgggcc gttgaacagt tcgctccagg aaaagctcgc
     3181 gacgtagcca tcccgcccgt gcgcatgcac gcagaccccg ccgcgctcgc gctggcgatc
     3241 gagctgcagg ccggcgagtt cgagcagcgc cgtcagccgc acgccctgcc agcagcgcag
     3301 gccgacggcc gggtcgaggc tgcgggctgc gcagctcaat gcccggcgca ctaccgtatg
     3361 ctgcaccagc ccctgcaagg ccccggcatc gaggcgcagc ggtcgcagca cccggccgtc
     3421 gagccgcagt gcgggtacgc ggacatccgc tgctgccggc atcaccgacg tcatgaaagg
     3481 cctcccggtc cggttttcta tatccgtacc aatacaataa atctgccatc gctcatgctg
     3541 catcgcatca ggctgaaacc gttgactttc cccgtcacag gcccggcgcc ggccctgccg
     3601 ttgcggccgc cggcagcgat gtcgcgactg cgaccgcccg ggtcgcgttt gtcggctttg
     3661 cgacgcgaaa accccggttc gcatgcggtt tgcagcgcgc ggagccggcc gtgagccgca
     3721 gccctgctga ctcaatgcaa ctcgaaggcc gtttctggct cgatcgcggt ggcaaaccgt
     3781 ttctcgggcc cgggcgcgtt gcgctgctgg agcgcatcga tgcctgccgc tcgatcgccg
     3841 aagccgcgcg ggcgatgggc atgggttaca agacggcctg ggatctggtc gatgcgatga
     3901 accagctcgc cgaacagccg ctggtcgtgc gcgtgaaggg cggccgtcag ggcggcggca
     3961 ccgagctgac cgattacggc cgcagcgtgg tcgcgacctt ccgcgcgctg gagcgcgatt
     4021 acgtggcgat gctcgaaacc ctcggcgccc gttaccccgg cttcggtgcc ttcgacgccc
     4081 atccgcgccc gccgcgcctg cgcaccagcg cccgcaacca gtggagcggc cacgtcaccg
     4141 gcatcgagcg ccacggcttc ggcgcgctgg tcacggtcga catcggtgcc ggcgcgccgg
     4201 tgcaggcggc gctcagcatg aacgccgtcg aggaactcgg cctcaccgac ggcagcgagg
     4261 tgcacgtgct ggtcaaggcg accgcgatcg gcatcggcgt ggtcgccggc tggggcggca
     4321 gcggcggcaa ccgtctgcac ggcgtcatcg agcacctgca gctcgatgcc ggcggcgagc
     4381 gcgcggagct gacgctcgca atcgccgcca gccgccacgt gctcgccatc atcgatccgg
     4441 ccatgatcgc cgcgctcggc ctgcaggccg gcgttgccgc ctgggcgcag ttcgagccgg
     4501 ccgcggtgat cctcgccgtc ggcgactgaa accccggcag cccttttccc ggacggcgat
     4561 gggtgcaaag ctgcgcgccc cgccgaatcc atccgccgcg atccgccgcg aggagaatcc
     4621 gccttgagca cggcctgcac tctcccggtc acgcagtgcg actgcatcgt catcggcgcc
     4681 ggcgccagcg gcctgctggt cgcccggctg ctggcgcgcg ccggcctgaa actcgtcgtg
     4741 ctggagcgcg gcgccatcgg tcgtgaatcc tcgtgggccg gcggcggcat cctgtcgccg
     4801 ctgtacccgt ggcgctacga cccggccgtc aacgcgctcg ccggctggag ccagcgcgcg
     4861 ttcgcagccc tcgccgccgg cctgcatgcg cagaccggca tcgacccgga atggacgccg
     4921 aacggcatgc tgtatctggg cggcctcgaa accgaagccg cgcgcgcctg ggccgtcgcg
     4981 caggcagcga gcctcgaagc cctgaccccg cccgatgccc gcggcatcga gccagttgtc
     5041 ccagttcatc cggccgttcg cgaaata
//
LOCUS       DAOQIE010000278         5055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9481_length_5055_cov_6.117400, whole genome shotgun sequence.
ACCESSION   DAOQIE010000278 DAOQIE010000000
VERSION     DAOQIE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9481_length_5055_cov_6.117400"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK144_14860"
     CDS             complement(<1..327)
                     /locus_tag="PK144_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HylD"
                     /protein_id="HNF67055.1"
                     /translation="MSAVPPPSPQLLRLAALVQLEQRARLATPAELAFVIVNETHGLL
                     AYRQAVLFDAGGRVEAVSGVALPDADGPYLQWLARAFAALPAAAGDEAPARRFGAAAL
                     PEALAAD"
     gene            complement(418..1158)
                     /locus_tag="PK144_14865"
     CDS             complement(418..1158)
                     /locus_tag="PK144_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF67056.1"
                     /translation="MRLAHRCIGLTFAALACAVASAAPESSAPATVRAQLVPVHGTTI
                     ASEMAGRIAELAPREGERFGEGDVLVRFGCAGQQAELARARATLDKKQRVHEVNGRLA
                     KLQSISRLELDVSAAEVAEAAAALKVAEAVVARCTIAAPWPGRVAERHVQRYQSVGEG
                     QPLLDVLDDRTLEVEMIVPSTSLAWLKPGHRFELAIDETGKRYAAELDRLGARVDPVS
                     QSIKIYGRLLAAAPELLAGMSGKVVLQP"
     gene            complement(1155..2798)
                     /locus_tag="PK144_14870"
     CDS             complement(1155..2798)
                     /locus_tag="PK144_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF67057.1"
                     /translation="MKPTPKPVCRTLAAALALALALTAGCTIRPEPLSDDEHRARVAE
                     DRKAIASEFVPVSAPLTLPEAIARALKYNYENRLALMESVLQDRQLSVANFAMLPRLA
                     VNAGYSARDNDLASVSQDVYPERHPRENNYQTSNDRAHHFADLTFTWNLLDFGVGYYQ
                     ARQQADRVLVARERRRKVVNNIVKEVTAAYWRAATAERLLPQVRPVLARAEQALRASR
                     SIEQDQLQPLIPTLEYRRNLLQVVGQLRKLAADLEVSKAQLAGLINVPAGAPLPIAVP
                     ARSEALPGAMQASLEQLETVGLHYRPDLREEAYQARIDRLGVRKEMIRLLPGLSLLGS
                     TSYDSNSYLLNRTWAEAGVRATWNLVNLAAGPSTIDAAEAQVEVDLWRRRALSIAAIV
                     QINVAWQQYRRALDGYDSADALNDIEGKLLRAVANAETAEAQSGFDRIRRELSALSAQ
                     LDRDRALAELYAALANVYVSVGIDPYDGPIDGVELPEMTRTVAANLALWRAGRLPEAA
                     LKAATEAAAPPAAPATADQPVPVPVPATAPKLADTGASR"
     gene            complement(2844..>5055)
                     /locus_tag="PK144_14875"
     CDS             complement(2844..>5055)
                     /locus_tag="PK144_14875"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNF67058.1"
                     /translation="TGTLTVRIVDDVPTANADTNSVTEDAGTPATGNVFASGSTGDAA
                     DRIGADTTATPVTTVSFGATAGTLGSPLSGAYGALTLNADGTYSYALDNNNPTVNALK
                     AGDSLSEVFSYTITDADGDTSTATLTITVQGRNDPPVAVDDSATTPEDTPISGNLLSN
                     DSDIDTPHADLRVQSFTIAGDPAVHAPGATAAIPGVGTLTISATGDWLFTPATDYSGP
                     VPVVTYTLTDGTLTDTATLTLDVTPVTDPPTLDLPPASVDEDGSVPLVILPAIGDTLP
                     ADLGIEHLTTITISGIPEGSRLSNAAGDPLLPAGGVLTLTPAQLAGLSFAPPRDYSGP
                     IVLNVTAVAQDGSAPPASITGPLSVTVRPVTDTPVIDVISASQTPDGALHLDLASHVT
                     DTVLTDTGTEHVDRVTISGLPEGTTVTLADGTQIPVIGGVATLAPDQLTGIRVQPPGG
                     FADDLVLQITSWAVDGSAPEASATTQVILGHGGGKVPPINPPLPPVIPPGPDPRPSGL
                     PAEPPEPIDTPFPFLFNYGRLGGFERDYEGHDQELYLYGQARDQLMVELRTDSFTQPK
                     DLFRHTNPAEHLSFEAVQPDGRPLPAWLKFDAGSLRFSGTPPAGFGGVDVVIIAYDSQ
                     GRSATALFRIRPAREVEPAPAVPVAPQLPPPPEPAPASAPPSDGAPPQATRSLPAADW
                     LAALGLHDDAAAHPAPALAPDAGHDGFSARLQQALPPTLAEQSRALADVLLSAD"
BASE COUNT          676 a         1686 c         1942 g          751 t
ORIGIN      
        1 gtcggcggcg agcgcctcgg gcagcgccgc ggcaccgaag cgccgcgccg gcgcctcgtc
       61 gccggccgcg gcaggcagcg ccgcgaacgc ccgcgccagc cactgcaggt aaggcccgtc
      121 ggcatccggc agcgcgacgc cggacacggc ctcgacgcga ccaccggcgt cgaacagcac
      181 cgcctgacgg tacgccagca ggccgtgcgt ctcgttgacg atcacgaacg ccagttcggc
      241 cggcgtggcg agtcgcgcgc gctgttcgag ctgcacgagc gcggcgaggc gcagcagttg
      301 cggggacggg ggcgggacgg cggacatcgg cggtggcggt gacgaggggg gatcagggcc
      361 gggagcccgc aggccgtccg gccggcgcgg tggatgcctg cgcaccgggt ccgatgctca
      421 cggctgcagc acgaccttgc cgctcatgcc ggccagcagc tcgggcgcgg cggcgagcag
      481 gcggccgtag atcttgatcg actggctgac cggatcgacg cgcgcgccga gccggtcgag
      541 ttcggccgcg tagcgcttgc cggtctcgtc gatggcgagt tcgaagcggt ggccgggttt
      601 cagccacgcg aggctcgtcg acggcacgat catctcgacc tccagcgtgc ggtcgtcgag
      661 cacatcgagc agcggctggc cttcgccgac cgactggtag cgctgcacgt ggcgctcggc
      721 aacccggccc ggccacggcg ccgcaatcgt gcagcgcgcg accacggcct cggccacctt
      781 cagcgccgcg gcggcctcgg cgacctcggc ggcggacacg tcgagttcga gccggctgat
      841 cgactgcagc ttcgcaagcc ggccgttgac ctcgtgcacg cgctgcttct tgtcgagcgt
      901 cgcccgcgcg cgcgccagtt cggcctgctg gccggcgcag ccgaagcgca ccagcacgtc
      961 gccttcgccg aagcgctcgc cctcgcgcgg tgccagttcg gcgatgcgcc cggccatctc
     1021 gctggcgatg gtcgtgccgt gcaccggcac gagctgcgcg cgcaccgtgg ccggcgcgga
     1081 cgactcgggg gccgcactgg cgacggcgca ggccagcgcg gcgaatgtca ggccgatgca
     1141 gcggtgcgcg agcctcaacg gctggctccg gtatcggcga gcttcggcgc ggtcgccggc
     1201 accggcaccg gcaccggctg gtcggcggtg gccggtgcgg ccggcggtgc tgcggcttcg
     1261 gtcgcggcct tcagtgccgc ctcgggcagc cggccggcgc gccacagcgc caggttggcg
     1321 gcgacggtgc gcgtcatctc cggcagctcg acgccatcga tcgggccgtc gtaagggtcg
     1381 atgccgaccg acacgtagac gttggcgagg gccgcataca gctcggcgag cgcgcggtcg
     1441 cgatcgagct gcgccgacag cgccgagagt tcgcggcgga tgcggtcgaa gccggactgc
     1501 gcctcggcgg tctcggcgtt ggcgaccgcg cgcagcagct tgccctcgat gtcgttgagc
     1561 gcgtcggcgc tgtcgtagcc gtcgagcgcg cgccggtact gctgccaggc gacgttgatc
     1621 tgcacgatcg ccgcgatgct gagggcgcgc cggcgccaca gatcgacctc gacctgcgcc
     1681 tcggcggcgt cgatcgtgct cgggccggcg gcgaggttga cgaggttcca ggtcgcgcgc
     1741 acgccggcct cggcccaggt gcggttcagc aggtaggagt tcgagtcgta gctggtcgag
     1801 ccgaggaggc tcaggcccgg cagcaggcgg atcatctcct tgcgcacgcc gaggcggtcg
     1861 atgcgcgcct gataggcctc ctcgcgcagg tcgggccggt agtgcaggcc gacggtttcg
     1921 agctgttcga ggctcgcctg catcgcaccg ggcagcgcct cgctgcgcgc gggcacggcg
     1981 atcggcagcg gcgcgccggc cggcacgttg atgaggccgg cgagctgcgc cttcgagact
     2041 tccagatcgg cggcgagctt gcgcagctgc ccgaccacct gcagcaggtt gcggcggtat
     2101 tcgagcgtcg ggatcagcgg ctgcagctgg tcctgctcga tgctgcggct cgcgcgcagc
     2161 gcctgctcgg cccgcgccag caccgggcgc acctgcggca gcagacgctc ggcggtcgcc
     2221 gcgcgccagt acgcggccgt gacttccttg acgatgttgt tgacgacctt gcgccggcgc
     2281 tcgcgcgcga ccagcacgcg gtcggcctgc tggcgggcct ggtaatagcc gacgccgaag
     2341 tcgagcaggt tccacgtgaa ggtcagatcg gcgaagtggt gcgcgcggtc gttcgaggtc
     2401 tggtagttgt tctcgcgcgg gtggcgctcg gggtagacat cctggctgac gctggcgaga
     2461 tcgttgtcac gcgccgaata gccggcgttg accgccagtc gcggcagcat cgcgaagttc
     2521 gccaccgaaa gctgccggtc ctgcagcacc gattccatca gcgccagccg gttctcgtag
     2581 ttgtacttca gcgcccgggc gatggcctcg ggcagcgtca gcggcgcgct gaccggcacg
     2641 aactcgctgg cgatggcctt gcgatcctcg gccacgcggg cgcggtgctc gtcgtcgctc
     2701 agcggttcgg ggcggatcgt gcagccggcg gtgagcgcga gggcgagggc gagggcagcg
     2761 gcaagcgtgc ggcagacggg cttgggcgtc ggcttcatga tcgggacggg tccggggcga
     2821 agtgttcggt tcggcatggg ggatcagtcg gcggagagca gcacatcggc gagcgcacgg
     2881 ctctgctcgg cgagcgtcgg cggcagcgcc tgctgcaggc gggcgctgaa accgtcgtga
     2941 ccggcatccg gtgcaagggc cggtgccggg tgggccgcag cgtcgtcgtg caggccgagg
     3001 gcggcgagcc agtcggcggc cggcagggag cgggtggcct gcggcggtgc gccgtcggag
     3061 ggcggtgccg atgccggggc cggttcaggc ggaggcggca gctgcggagc caccggcacg
     3121 gccggtgccg gctcgacttc ccgggccggg cggatacgga acagcgccgt ggcgcttcgg
     3181 ccctgcgagt cgtaggcgat gatcaccacg tcgacaccgc cgaagcctgc gggcggcgtg
     3241 cccgagaagc gcaggctgcc ggcatcgaac ttcagccacg ccggcagcgg ccggccgtcg
     3301 ggctgcacgg cctcgaagct caggtgctcg gccggattcg tgtgccggaa caagtccttg
     3361 ggctgcgtga agctgtcggt tcgcagctcg accatcagct gatcgcgcgc ctggccgtac
     3421 aggtacagct cctgatcgtg accttcgtag tcgcgctcga acccacccag gcggccgtag
     3481 ttgaacagga acgggaacgg cgtatcgatc ggctcgggcg gctccgcggg caggccggac
     3541 gggcgtgggt ccgggccggg cgggatgacc ggtggcaggg gcggattgat cggcggaacc
     3601 ttgccgccgc cgtggccgag gatcacctgc gtcgtggcgc ttgcttcggg tgcgctgccg
     3661 tcgaccgccc agctcgtgat ctgcaggacc agatcatcgg caaagccgcc gggcggctgc
     3721 acgcggatgc cggtcagctg atccggcgcc agggtggcga caccaccgat cacgggaatc
     3781 tgcgtgccat cggccagcgt gaccgtcgtt ccctccggca gcccggaaat cgtcacccgg
     3841 tccacatgct cggtgccggt atcggtcagc accgtgtccg tgacatggct ggcgagatcg
     3901 aggtggaggg cgccgtcggg ggtctgcgag gcactgatga catcgattac cggtgtatcg
     3961 gtcacgggcc ggaccgtcac gctcagcggg ccggtgatgc tcgccggcgg cgcactgcca
     4021 tcctgcgcaa ccgccgtgac attcagcacg atcgggccgg agtaatcccg cggcggcgcg
     4081 aaactcaggc cggcaagctg ggccggagtc agcgtcagca cgccgccggc cggcagcagc
     4141 ggatcgccgg cggcgttgct gagccggctg ccctccggaa tgcccgagat cgtgatggtg
     4201 gtcaggtgtt cgatgccgag atcggccggc agcgtgtcgc cgatcgccgg caggatcacg
     4261 agtggcaccg agccatcctc gtcgacgctc gccggcggca gatccagcgt cggcggatcg
     4321 gtgaccggcg tcacgtcgag cgtcagcgtc gcggtatcgg tcagcgtgcc gtcggtgagc
     4381 gtgtaggtga cgaccggcac ggggccggaa tagtcggtgg ccggcgtgaa cagccagtcg
     4441 ccggtcgccg agatcgtcag cgtgccgaca ccgggaatcg cggcagtcgc cccgggcgcg
     4501 tggacggcgg gatccccggc gatcgtgaac gactgcacgc gcagatcggc atgcggcgtg
     4561 tcgatgtcgc tgtcattgga gagcaggttg ccgctgatcg gcgtgtcttc gggcgtcgtg
     4621 gcactgtcgt cgacggcgac cggcgggtcg ttgcgaccct ggaccgtgat cgtcagcgtc
     4681 gcggtcgagg tgtcgccatc ggcatcggtg atcgtgtagc tgaagacttc gctcagcgaa
     4741 tcgccggcct tgagcgcgtt gacggtcggg ttgttgttgt cgagggcata cgaatacgtg
     4801 ccatcggcat tgagcgtcag cgcgccatag gcgccggaca gcgggctgcc gagcgtgccc
     4861 gcagtggcgc cgaagctcac ggtcgtcacc ggcgttgccg tcgtatcggc gccgatgcgg
     4921 tcggctgcgt cgccagtact tccggacgca aagacgttgc cggtggccgg cgtgcccgca
     4981 tcctccgtga ccgaattggt atcggcattc gccgttggca cgtcatcgac gatgcgcacg
     5041 gtcagcgtgc cggta
//
LOCUS       DAOQIE010000279         5028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9573_length_5028_cov_5.857229, whole genome shotgun sequence.
ACCESSION   DAOQIE010000279 DAOQIE010000000
VERSION     DAOQIE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9573_length_5028_cov_5.857229"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..663)
                     /locus_tag="PK144_14880"
     CDS             complement(67..663)
                     /locus_tag="PK144_14880"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HNF67059.1"
                     /translation="MASHRGVEAAMLALEAALRREQPAELAAFAPVRLLGSADLARQD
                     LGGLLGLYVYRVEIDPATRNRYHVPPPGSNAAVQPLLPLVLHFLLFPWVANASEELRL
                     LGWAMQALARIAELDAARIGAFDPDWLDNERASVQPEDLSIEDLLRIWDGLPLRYRPS
                     AAYAVRTVFVREPPQAGAGRVRTRVFGLDTETGAGAGP"
     gene            complement(1080..1457)
                     /locus_tag="PK144_14885"
     CDS             complement(1080..1457)
                     /locus_tag="PK144_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF67060.1"
                     /translation="MQIIATSEAPAAIGPYSQGIVVNGLLYTSGQIPLNAAGEFVEGE
                     IDVQTEQVIDNLAAILAAAGATLADVVKTTVFVTDLNDFARLNEIYGQRFGEHRPARS
                     TVQVARLPRGAKVEIEAVVKLPG"
     gene            complement(1569..1901)
                     /locus_tag="PK144_14890"
     CDS             complement(1569..1901)
                     /locus_tag="PK144_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67061.1"
                     /translation="MNQAEATSTGTGLDHPAAIAALTAYATMERSKSAHFEMLRALEE
                     KYQKYGRPSADEEAELAGRLAGHDRAVRDFREAVAALRAADETAFAAVMAELHSEASR
                     ATGTPTEQ"
     gene            1995..3353
                     /locus_tag="PK144_14895"
     CDS             1995..3353
                     /locus_tag="PK144_14895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF67062.1"
                     /translation="MTFRTAPAIAEARALLALAVPLMLAQLAQMLMGYTDTLMVARVG
                     ALELAAVGTGLALWHPLYLAVTGLLMAESALIARLAGAGRADAAGGLRRHARALALAC
                     ALPAVVLMNHAGPLLGALGVDAAIVPVAQDYLGALSFGVPGAFWYLAERYAIEGLGRT
                     RPVLVAGLAAFVLNIGLNWLLIYGHLGLPALGARGCGYATAACFWLEACGLAWLNRRL
                     PALRSHSPSPDAPGLRALLGLGLPIAAAVLFEVGIFCAVALALGRLGADAVAAHQIAL
                     TTASLTFMIPLALAAAITIRIGGALGAGDVPAARRAGWTGIALAAAFMALSATGIGFG
                     APWIAALYTGDAAVAALAVQLLHVAAAFQISDGLQVSAGGALRGLHDTRGPMLITLFA
                     YWLIGFPFGWWLAFGHGQGAPGLWFGLVAGLSCAALLLNLRFRRGLARLQTAAPVLPE
                     SA"
     gene            3353..3838
                     /locus_tag="PK144_14900"
     CDS             3353..3838
                     /locus_tag="PK144_14900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF67063.1"
                     /translation="MNAAVDLQPAGFPADRAAVEALFREYEQALGIDLCFQNFADELA
                     TLPGRYAAPAGTLLLARTGSEGDALLGCVALRPLGDGIAELKRLYVRPAGRGLGLGRR
                     LSEAALAHARAAGHRAVRLDTLATMTEARALYASLGFVPIAAYCHNPLPGVMYMELRL
                     E"
     gene            complement(3870..4502)
                     /locus_tag="PK144_14905"
     CDS             complement(3870..4502)
                     /locus_tag="PK144_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNF67064.1"
                     /translation="MADPYTRIAFTDGVRRLQEHYGTRIEHHDRERGDEQPARLGARE
                     ARFIAARDTFFMATVSETGWPYVQHRGGPPGFLKVLGATTLGYADFRGNLQYVSMGNL
                     GHDGRVALFLIDFRQKLRLKVLGHARFVDLGQASTELVQQIDLGNYFAEVERAVLIEV
                     AAFEWNCPRHITPRYTGDEVRALVEPLQARIAELERQLAARDRDGEVPGG"
BASE COUNT          669 a         1715 c         1835 g          809 t
ORIGIN      
        1 ccggtgccga actcgcatcg accgcgcaga gcagcgcgtg gccgtgcatc accagccact
       61 cggcgctcac gggccggctc cggcaccggt ttcggtatcg agcccgaaca cccgcgtgcg
      121 cacgcgcccg gcgccggctt gcggcggctc gcgcacgaac accgtgcgca ccgcgtaggc
      181 cgcactcggg cggtagcgca gcggcaggcc gtcccagatg cgcagcagat cctcgatcga
      241 caagtcctcg ggctgcacgc tcgcgcgctc gttgtcgagc cagtccggat cgaaggcgcc
      301 gatgcgcgcg gcatcgagtt cggcgatgcg cgccagcgcc tgcatcgccc agccgagcag
      361 gcgcagttcc tcggaggcgt tggcgaccca cggaaacagc aggaagtgca gcaccagcgg
      421 cagcagcggc tgcaccgcgg cgttcgagcc gggcggcggc acgtgatagc ggttgcgcgt
      481 ggccgggtcg atttcgacgc ggtagacgta cagcccgagc aggccgccga ggtcctggcg
      541 cgccagatcg gcgctgccga gcaggcgcac cggcgcgaac gcggcaagct ccgccggctg
      601 ctcgcggcgc agcgccgctt cgagcgccag catcgccgcc tcgacgccgc ggtggctcgc
      661 catgtcagcg cccccgcgtc cggggcagga acggatggat atcggggccg gtatcggcgg
      721 ggagcatccg gcaatcgagc gcgagccgat ccactgcagc ctccgatggc aaggtcacgc
      781 cgggctgagg ggcgccgaag cggactcagc cgtcagggcc gtcagggccg tcaggcttga
      841 gacgggttgc gacggaacct agcagtggat gtcgcgatga caacgcgccg ggcattaagg
      901 atcggaaagg attgctgtcc ggccggcgac cggcttcggg tcgccggtgc ctgaggcggc
      961 gtgtccggca agcagcaggg tgcgcggaat gacccgctgc cggtgcaacg gctcccggcg
     1021 ttgcgggccg tggttccggg agtggaggcg atgccggcat ggacccgccg gccgccgtct
     1081 cagcccggca gcttcacgac cgcctcgatc tccaccttcg caccgcgcgg caggcgggcg
     1141 acctgcacgg tcgagcgtgc cgggcgatgc tcgccgaagc gctggccgta gatttcgttg
     1201 aggcgtgcga agtcgttcag gtcggtcacg aacaccgtgg tcttgaccac gtcggcgagc
     1261 gtcgcgccgg cggcggcgag gatcgcggcg aggttgtcga tgacctgctc ggtctgcacg
     1321 tcgatctcgc cttcgacgaa ctcgccggcg gcgttcagcg gaatctggcc ggaggtgtac
     1381 agcaggccgt tgacgacgat gccctgcgaa tacgggccga tcgcggcggg ggcttcggac
     1441 gtggcgatga tctgcatgag gcattcggct ccggttcggg aacggatgcc ggccggaggc
     1501 gcgccggccg gggcggggat gaccatgctg gccgcgggga cttgccggcg caagcaggcc
     1561 ggggcggctc actgctcggt cggggtgccg gtcgcgcgcg aggcctcgct gtgcagctcg
     1621 gccatcaccg cggcaaacgc cgtctcgtcg gctgcgcgca gcgccgcgac cgcctcgcgg
     1681 aagtcgcgca cggcgcggtc atgcccggcg agccgtccgg cgagttcggc ttcctcgtcg
     1741 gccgagggcc ggccgtactt ctgatacttc tcctccagcg cccgcagcat ctcgaaatgc
     1801 gcgctcttgc tgcgctccat cgtggcgtag gcggtcagcg cagcgatggc ggccgggtgg
     1861 tcgagtccgg tgccggtgct ggtggcttcg gcttggttca tgatgcgcgg ctcctgcgga
     1921 cggcatgttt cagatgctcc gcgatgatgg ggggatcagg cggcggatac gcggccgccg
     1981 ccgggctgcg catgatgaca ttccgcaccg cgcctgccat cgccgaagcg cgcgcgctgc
     2041 tcgcactcgc cgtgccgctg atgctcgcgc agctcgcgca gatgctgatg ggctacaccg
     2101 acacgctgat ggtcgcgcgc gtcggcgcgc tcgaacttgc cgcggtcggc accgggctcg
     2161 cgctctggca tccgctgtac ctggccgtca ccggcctgct gatggccgag agcgcgctga
     2221 tcgcccgcct cgccggcgcc ggccgtgccg atgcggccgg cgggctgcgc cggcatgcgc
     2281 gcgcgctggc gcttgcctgc gcgctgccgg ccgtcgtcct gatgaatcac gccgggccgc
     2341 tgctgggcgc gctcggcgtc gatgccgcga tcgtgccggt cgcgcaggac tatctcggcg
     2401 ccctgtcctt cggcgtgccg ggggcgttct ggtatctggc cgagcgttac gcgatcgaag
     2461 gtctcggccg cacgcggccg gtgctggtcg cgggcctcgc ggcgttcgtg ctgaacatcg
     2521 gcctcaactg gctgctgatc tacggccatc tcggcctgcc ggcgctcggg gcgcgcggct
     2581 gcggctatgc gaccgccgcc tgcttctggc tggaagcctg cgggctcgcg tggctgaacc
     2641 gccggctgcc ggcgctgcgc agccacagtc cttcgccgga tgcccccggc ctgcgcgcgc
     2701 tgctcgggct cggcctgccg atcgcggcgg cggtgctgtt cgaggtcggc atcttctgcg
     2761 ccgtcgcgct cgcactcggc cggctcggcg cggacgcagt cgccgcccac cagatcgcgc
     2821 tgaccaccgc gagcctgacc ttcatgatcc cgctggcgct ggccgcggcg atcacgatcc
     2881 gcatcggcgg tgcgctcggg gccggcgacg tgccggcggc gcgccgggcc ggctggactg
     2941 gcatcgcgct cgccgcggcg ttcatggcgc tgtcggcgac cggcatcggc ttcggtgcgc
     3001 cgtggatcgc ggcgctctac accggcgatg ccgcggtcgc ggcgctggcc gtgcagctgc
     3061 tgcacgtcgc cgcggccttc cagatctccg acggcctgca ggtgtcggcc ggcggcgcgc
     3121 tgcgtggcct gcacgacacg cgcgggccga tgctgatcac gctgttcgcg tactggctga
     3181 tcggtttccc gttcggctgg tggctggcct tcgggcacgg gcagggcgcg cccggtctgt
     3241 ggttcgggct ggtcgcgggc ctgagctgcg cggcgctgct gctgaacctg cgcttccgtc
     3301 gcgggctggc gcgcctgcag actgcggctc ccgtcctgcc cgagtctgcg tgatgaatgc
     3361 cgccgtcgat ctgcagcccg ccggatttcc ggccgatcgc gccgccgtcg aggcgctgtt
     3421 ccgtgaatac gaacaggcgc tcgggattga tctctgcttc cagaacttcg ccgacgaact
     3481 ggcgacgctg cccggccgct acgccgcccc ggccggcacg ctgctgctcg cgcgcaccgg
     3541 cagtgaaggg gatgcgctgc tcggctgcgt ggcgctgcgc ccgctcggcg acggcatcgc
     3601 cgagctgaag cgcctgtacg tgcgcccggc cgggcgcggc ctcggactcg gtcggcgcct
     3661 cagcgaagcg gcgctcgcac acgcccgcgc cgccggccac cgtgccgtgc gcctcgacac
     3721 gctggcgacg atgaccgagg cgcgcgcgct gtacgcgagc ctcggcttcg tgccgatcgc
     3781 cgcctactgc cacaacccgc tgccgggcgt gatgtacatg gaactgcggc tggaatgagg
     3841 gccgcgtccc ttgccggccc gctccggttt cagccgcccg gcacctcgcc gtcgcgatcg
     3901 cgcgccgcga gctggcgctc cagctcggcg atgcgcgcct gcagcggttc gaccagcgcc
     3961 cgcacctcgt cgcccgtata gcgcggcgtg atgtgccgcg ggcagttcca ctcgaaggcc
     4021 gcgacctcga tcagcacggc gcgctcgacc tcggcgaaat agttgccgag gtcgatctgc
     4081 tgcacgagct cggtcgatgc ctgcccgaga tcgacgaagc gcgcatgtcc gagcaccttc
     4141 agccgcagct tctgccggaa atcgatcagg aacagcgcga cgcgcccgtc atggccgagg
     4201 ttgcccatgc tcacgtactg caggttgccg cggaaatcgg cgtaaccgag cgtcgtggca
     4261 ccgagcacct tcaggaatcc gggcggcccg ccgcggtgct gcacgtacgg ccagccggtc
     4321 tcgctgaccg tggccatgaa gaaggtatcg cgcgccgcaa tgaagcgcgc ctcacgcgcc
     4381 ccgagccgcg ccggctgttc gtcgccgcgt tcgcggtcgt ggtgttcgat gcgcgtgccg
     4441 tagtgctcct gcaggcggcg cacgccgtcg gtgaaggcga tgcgggtgta gggatcggcc
     4501 atggcggggt cttcatgttg cggacgggag cgaggagcag gacgcgggaa gagaagacgt
     4561 gcgtcgtccg cgcgggatgg atatagctgg tctggcgggg gaactgcacg ggaggggacg
     4621 gttggccacg ggtggcagat cactgcagct gatgcgtggt gcgcggaaga caggttacgg
     4681 cgcgcaggga gatatgaagc tgatttgaca tggtgttatg cgcacctgat cgtcatgcgc
     4741 gtgctataag aagatggcga gaataatcta attattctgt ctgcaaaaaa tgacggagac
     4801 gaaagcgcag ttgttcgcgg tctttggttt cgcattccca gatcacaagt actcgccacc
     4861 ccaaagattg cagttgttgt tgctgcagtc gatcacgctc tatgttcgat tgtcgttttt
     4921 ttgcccagaa atctgtgttg gatgtaggcg cagcccctcg gtaacagtca tgggcgtgcc
     4981 agaagcaacc atgcacgaaa acgacagagc gcctgcgtgg caagacga
//
LOCUS       DAOQIE010000280         4981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9702_length_4981_cov_4.775274, whole genome shotgun sequence.
ACCESSION   DAOQIE010000280 DAOQIE010000000
VERSION     DAOQIE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9702_length_4981_cov_4.775274"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..645)
                     /locus_tag="PK144_14910"
     CDS             complement(181..645)
                     /locus_tag="PK144_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67065.1"
                     /translation="MENRISVSLPADQLKTVLELIKQLEAALPFLMALSPDERKSLPR
                     MGDASRPFVDKSLKLAQAHNDFLPRSFDTGEFAADVQLYNALDPVRPRLSPLVEKLDD
                     TLTVTGSDAYLAALEVYRYASTNGEAAGLDELIALMGKRFARRSRSTKPETP"
     gene            complement(986..1420)
                     /locus_tag="PK144_14915"
     CDS             complement(986..1420)
                     /locus_tag="PK144_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNF67066.1"
                     /translation="MAQVRYEQDFAAWTAEQSRLLREGRLVELDLAHLAEEIEDMGKN
                     SQRALLSRLQELLLHLLKWQYQPGRRGNSWRASIRKQRQGIEDLLEDSPSLQPRLAAA
                     YGKCYARARLDAADETGLALTTFAESCPWSLDEVLDPAWLPD"
     gene            complement(1632..2135)
                     /gene="greB"
                     /locus_tag="PK144_14920"
     CDS             complement(1632..2135)
                     /gene="greB"
                     /locus_tag="PK144_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNF67067.1"
                     /translation="MSRYRPPAPASSPYITRAGYERLSAELQFLWKVRRPEVTRAVAE
                     AAAQGDRSENAEYTYGKKQLREIDRRVRYLSKRLDVLTVVDRIPDDRTRVYFGAWVEY
                     ETEAGEAREVRIVGADEFDPAQHWISVDSPLARALLKRQDGDEVRVQLPEGEATVWIV
                     GIRYGEG"
     gene            complement(2141..3478)
                     /locus_tag="PK144_14925"
     CDS             complement(2141..3478)
                     /locus_tag="PK144_14925"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNF67068.1"
                     /translation="MSSIKSWFKDHQIVEVEAIIPDMSGVARGKILPAEKYYQDKGLR
                     LPEGIFLQTITGDAPDDYYDVISAADGDIVLRPDLDTICLVPWATEPTAQIIHDAYYG
                     DGRVVDIAPRHVLQRVLRLYAERGWKPVVAPELEFFLVKPNTDSDYPLEPPIGRSGRR
                     ETGRQSYGIDAVNEFEPLFEDVYDYCEKQGLGIDTLIHESGAAQLEINLLHGDALALA
                     DQAFRFKRTMREAAMRHQIYATFMAKPMQHEPGSAMHIHQSIVGLDDGRNVFSDEDGN
                     PTPLFYAHIAGLQKYLPSAMALIAPNVNSYRRINRTDVAPINVQWGYDNRTAAFRVPQ
                     ASPQSRRIENRVPGADANPYLAMAASLACGYLGMIERLQPTQPWEGSAHDAPYALPRS
                     VHEALRTLTSCDALKQVLGERFVTAFVSVKQAEYEAFFQVISSWEREYLLLNV"
     gene            3631..4914
                     /locus_tag="PK144_14930"
     CDS             3631..4914
                     /locus_tag="PK144_14930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF67069.1"
                     /translation="MRTLAHARSWYEDALPPEPPAPALAGDVRADVAVLGAGLTGLNA
                     ALELAARGLRVVLVEAARAGAGGSGRSGGQVLGDYACGMETLRGLLGREAAAQLWQWS
                     LEAVARVKNHIARHAIACDWRDGCATLALKPRQVRALRDWQAQAAHDYGYDGLRWLAA
                     GELGELVASPRYCGALLDTGAGHLQPLAYTRALAQAARAAGVTLHEHSPVLRIETGPR
                     PKLVTAGGTVEADQLVIAGGALPARLAPEPAAKVLPVGTYIVATGPLGADVATALLPA
                     DAALCDANFVLDYYRRSADHRLLFGGRVSYSGLESPGLAAGMRRRLLAVFPQLPRTLA
                     ITHAWGGHVDITVNRAPHFGRIGSRVWFAQGFSGHGMALSNLAGRVLAEAVSGQLERF
                     DVFAGIPHRAFPGGGRLRVPLLMLAMLAFRLRDRL"
BASE COUNT          672 a         1712 c         1803 g          794 t
ORIGIN      
        1 gaccattcac aacgcggcaa acaccgagga actcttgcag atcgcgcttg aagccttgcg
       61 cgcaaggctt tttcccttgc acgtcgcgct cgaagccttg cgcttcgcgc tttgagcctt
      121 gcgcctgctg tcggcgaggg aatgaccggg gcgcgcagcc ccggtcgtgg ggcgggaggc
      181 ttacggcgtc tcgggtttgg tgctgcgcga gcggcgggcg aagcgcttgc ccatcagcgc
      241 gatcagttcg tcgaggccgg cggcctcgcc gttcgtgctc gcgtagcgat agacctcgag
      301 cgcggcgagg taggcgtcgc tgccggtgac ggtcagcgtg tcgtcgagct tctcgaccag
      361 cggcgacagg cgggggcgca cggggtcgag ggcgttgtaa agctgcacgt cggcggcgaa
      421 ctcgccggtg tcgaagctgc gcggcagaaa atcattgtgt gcctgcgcga gtttcagcga
      481 cttgtcgacg aacggccggc tggcatcgcc catgcgcggc aggctcttgc gctcgtcggg
      541 gctgagcgcc atcaggaacg gcagggcggc ttcgagttgc ttgatcagct cgagtacggt
      601 cttgagctga tcggcgggca ggctgacgga gatacggttt tccatgtgcg gcactcctta
      661 tggtggcact gcgaacgacc cgcaccgccc ggcgcgggct ggacgcgagt atcggcagcc
      721 acccggcccc tcgcactccg gcagatgccg tattcgtcgc gctttgtcgc agcggtccgg
      781 agcctgatgc cggcttgcgg tgcgcccgcc tgaaaacggc ctgcgccgtg cccggcccgg
      841 gctgcgggcc atgcccttgc gtccgccgat accgcgtgcc gggcagccgc ttgcgccaca
      901 gcggccggcg ctgccgaacg gcccgcacgc acggcaagct gcgcgcagcc ggtgcgcacg
      961 cagtccgccc gcggttcgcg tgctttcagt ccggcagcca ggccgggtcc agcacctcat
     1021 cgagagacca cgggcaggac tcggcaaacg tggtcagtgc cagtccggtt tcatcggcgg
     1081 catccagacg ggcgcgggcg taacacttcc cgtaggcggc agccagacgc ggctgcaggc
     1141 tcggactgtc ttcgagcaga tcctcgatgc cctgacgctg cttgcggatc gaggcccgcc
     1201 agctgttgcc gcgccgcccc ggctggtatt gccacttcag caagtgcagc agcagttcct
     1261 gcaggcggct cagcagggcg cgctggctgt tcttgcccat gtcctcgatc tcctcggcca
     1321 ggtgtgcgag gtcgagttcg accagacggc cttcgcgcag cagccgcgac tgctccgccg
     1381 tccaggcggc aaaatcctgc tcgtatcgga cctgagccat gacagcctcc cgcagaggtt
     1441 tcgtcgaatt tcattccggt cacgacgcag cagccggaga ttcccgccgc tgccggcgcg
     1501 aggcagggtc cgggccggcc cggcacgcac gcgcaggcca gcggcaggac ggtcagaaga
     1561 tacaagacct tgctccaccg gcctcctgca cgctgcgctc atgcccgtac cgtgcatggc
     1621 cgtcgtcgcc gtcagccctc gccgtagcga atcccgacga tccacaccgt cgcctcgccc
     1681 tccggcagct gcacgcgcac ctcgtcgccg tcctgacgct tgagcagggc gcgggcgagc
     1741 ggcgagtcga cgctgatcca gtgctgcgcc gggtcgaact cgtcggcgcc gacgatgcgc
     1801 acctcgcggg cttcgccggc ttcggtctcg tactcgaccc aggcgccgaa gtacacgcgc
     1861 gtgcggtcgt cggggatgcg gtcgacgacg gtcagcacgt cgaggcgctt gctgaggtag
     1921 cgcacgcgcc ggtcgatctc gcgcaactgc ttcttgccgt aggtgtattc ggcgttctcg
     1981 gagcgatcgc cctgcgcggc ggcctcggcg accgcgcgcg tgacctcggg gcgtctgacc
     2041 ttccacagga actgcagctc ggcgctcagg cgctcgtagc cggcgcgggt gatgtacggc
     2101 gacgaggccg gggcgggcgg gcggtagcgg gacatgggtt tcagacgttc agcagcaggt
     2161 attcgcgctc ccaggagctg atgacctgga agaaggcttc gtactcggcc tgcttgaccg
     2221 agacgaaggc ggtgacgaag cgctcgccga gcacctgctt cagggcgtca cagctcgtca
     2281 gcgtgcgcag cgcctcgtgc acgctgcgcg gcagcgcata cggtgcgtcg tgggcgctgc
     2341 cctcccaggg ctgggtcggc tgcaggcgtt cgatcatgcc gaggtagccg caggcgagcg
     2401 aggcggccat cgccagatag gggttggcgt cggcgccggg cacgcggttc tcgatgcgcc
     2461 gcgactgcgg gctggcctgc ggcacgcgga aggcggcggt gcggttgtcg tagccccact
     2521 gcacgttgat cggcgccaca tcggtgcggt tgatgcgccg gtagctgttg acgttcggcg
     2581 cgatcagcgc catcgcgctc ggcaggtact tctgcaggcc ggcgatgtgg gcgtagaaca
     2641 gcggcgtcgg gttgccgtcc tcgtcgctga acacgttgcg gccgtcgtcg aggccgacga
     2701 tgctctggtg gatgtgcatg gcgctgccgg gctcgtgctg catcggcttg gccatgaagg
     2761 tcgcgtagat ctggtgacgc atcgccgcct cgcgcatcgt gcgcttgaag cggaaggcct
     2821 gatcggcgag cgcgagcgca tcgccgtgca gcaggttgat ctccagctgc gcggcgccgg
     2881 attcgtggat cagcgtgtcg atgccgaggc cctgcttctc gcagtagtcg tagacatcct
     2941 cgaacagcgg ctcgaattcg ttgacggcgt cgatgccgta ggattgccgg ccggtttcgc
     3001 gccggcccga gcgcccgatc ggcggctcca gcgggtaatc cgaatcggtg ttcggcttga
     3061 ccaggaagaa ttcgagttcg ggcgccacga ccggcttcca gccgcgctcg gcgtagagcc
     3121 tgagcacgcg ctgcagcacg tggcgcggcg cgatgtcgac gacgcggccg tcgccgtaat
     3181 aggcgtcgtg gatgatctgc gcggtcggct cggtcgccca gggcacgagg cagatggtgt
     3241 cgaggtcggg gcgcagcacg atgtcgccgt cggcggcgct gatcacgtcg tagtaatcgt
     3301 cgggggcatc gccggtgatc gtctgcagga agatgccttc gggcaggcgc aggcccttgt
     3361 cctgataata cttttcggcc ggcaggatct tgccgcgcgc gaccccggac atgtccggga
     3421 tgatggcctc gacctcgacg atctgatggt ccttgaacca gctcttgatg gaactcatgc
     3481 ggggaatccg gggaaaccgc ggctgcggcc gtccgcgagc ctagtcgagc ggctcgcgcg
     3541 caaacaaggc gcgcaccgcg agcggctgcc cggcttctgc aaaggccgat gaacgcgccg
     3601 atgaatgcgc cgaccggggg aatccacgcc atgcgcacgc tcgcccacgc ccgctcctgg
     3661 tacgaggacg cgctgcctcc cgagccgccg gccccggcac tcgccggcga cgtgcgcgcc
     3721 gatgtcgccg tgctcggcgc cggcctcacc ggcctcaacg ccgcgctcga actggcggcg
     3781 cgcggcctgc gcgtggtgct ggtcgaggcg gcgcgcgccg gggccggcgg ctccgggcgc
     3841 agcggcgggc aggtgctcgg cgactacgcc tgcggcatgg aaacgctgcg cgggctgctc
     3901 gggcgcgagg ctgccgcgca gctctggcag tggtcgctgg aggcggtcgc gcgcgtgaag
     3961 aaccacatcg cccgccacgc catcgcctgc gactggcgcg acggctgcgc gacgctcgcg
     4021 ctcaagccgc ggcaggtgcg ggcgctgcgc gactggcagg cgcaggcagc gcacgactat
     4081 ggctacgacg gcctgcgctg gctcgccgcc ggcgaactcg gcgagctggt cgcgagcccg
     4141 cgctactgcg gcgcgctgct cgacaccggt gccggccacc tgcagccgct ggcgtacacg
     4201 cgcgcgctgg cgcaggcggc acgcgcagcc ggcgtcacgc tgcatgagca cagcccggtg
     4261 ctgcgcatcg aaaccgggcc gcggccgaag ctcgtgacgg ccggcggcac ggtcgaggcc
     4321 gatcagctcg tgatcgccgg cggtgcgctg ccggcgcggc ttgcgccgga accggcggcg
     4381 aaggtgctgc cggtcggcac ctacatcgtc gcgaccgggc cgctcggtgc cgacgtggcg
     4441 acggcgctgc tgccggcgga cgccgcgctc tgcgatgcca acttcgtgct cgactactac
     4501 cggcgctcgg ccgatcaccg gctgctgttc ggcgggcgcg tcagctacag cggcctggaa
     4561 tcgccgggcc tcgcggccgg catgcgccgg cgcctgctcg cggtgttccc gcaactgccg
     4621 cgcacgctcg cgatcacgca cgcctggggc ggccacgtcg acatcaccgt caaccgcgcc
     4681 ccgcacttcg ggcgcatcgg cagccgggtc tggttcgcgc agggcttttc cggccacggc
     4741 atggcgctct cgaacctcgc cggccgcgtg ctcgccgagg cggtcagcgg ccagctcgaa
     4801 cgcttcgatg tgtttgccgg catcccgcac cgggccttcc cgggcggcgg ccggctgcgc
     4861 gtgccgctgc tgatgctggc gatgctggcg ttccggctgc gggaccggct gtagggcgcg
     4921 ggcggatcag tcctcgcccg ccccgcgcat ccacgcccgc agcagcccga tcagcagcgc
     4981 c
//
LOCUS       DAOQIE010000281         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9727_length_4973_cov_7.951403, whole genome shotgun sequence.
ACCESSION   DAOQIE010000281 DAOQIE010000000
VERSION     DAOQIE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_4973_cov_7.951403"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK144_14935"
     CDS             complement(<1..424)
                     /locus_tag="PK144_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HNF67070.1"
                     /translation="MTSILTGALAALLYLATGTLLGVRLARVNQPHQPARAPALALGL
                     VAIVLHAFVLGQTVVQPEGMNLGFFNALSFTGWLIALLLLASSLARPVENLGIVLLPF
                     TATTVVLALAFPSTRLVTEAAQWRLELHILISITAYALL"
     gene            555..1949
                     /gene="ffh"
                     /locus_tag="PK144_14940"
     CDS             555..1949
                     /gene="ffh"
                     /locus_tag="PK144_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNF67071.1"
                     /translation="MFENLSERLLRTVKHLRGQARLTEDNIRDALREVRMALLEADVA
                     LPVVRSFIEQVRVRAVGQEVMTALSPGQVLVKIVNEELVRLMGEANEALDLRAQPPAV
                     VLMAGLQGSGKTTSTAKLARFLREREKKKVLVVSCDVYRPAAIQQLATLAKEVQVEWF
                     ASEGGQQPVAIAQAALAYAKRQFFDVLIVDTAGRLHVDAAMMEEIQALHAAVTPVETL
                     FVVDAMTGQDAANTAKAFHEALPLTGVVLTKTDGDARGGAALSIRYLTGKPIKFLGVG
                     EKTAALEPFHPDRVASRILGMGDVLSLVEEVERKVDKEKALKLAEKVKSGKSFDFEDF
                     REQMEQMLAMGGMAGLLDKLPGMDKVPDDLKKQANDKQVRRLIGIVNSMTPQERRFPD
                     LIKGSRKRRIAAGAGVQVQEINRLLKQFEEMRRMMKKMSGGGMMKLMRAMKGKLPMGG
                     LGGLGGPGGLPPFK"
     gene            2114..2374
                     /gene="rpsP"
                     /locus_tag="PK144_14945"
     CDS             2114..2374
                     /gene="rpsP"
                     /locus_tag="PK144_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNF67072.1"
                     /translation="MVTIRLARGGAKKRPFYHVVVTDSRNRRDGRYIERLGFFNPIAR
                     GGEVRLNLDAGRVQYWQGNGAQVSERVAALLKEFDKAQAAAA"
     gene            2404..2913
                     /gene="rimM"
                     /locus_tag="PK144_14950"
     CDS             2404..2913
                     /gene="rimM"
                     /locus_tag="PK144_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965407.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNF67073.1"
                     /translation="MAAADSWVVLGRVAGLWGVRGGLKIWSDTQPRAAICRYRPLFLG
                     RDGQWREVVPVLGREQGKGIVMQFDGVEDRDAAAALIGCEIAIRREQLPAPAPGEYYW
                     TDLVGLRVETLDGIELGHVDHLFETGSNDVIVVRGERERLIPFLRGSVIQDIDLEAGR
                     MRVDWDPDF"
     gene            2938..3756
                     /gene="trmD"
                     /locus_tag="PK144_14955"
     CDS             2938..3756
                     /gene="trmD"
                     /locus_tag="PK144_14955"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNF67074.1"
                     /translation="MRIDVVTLFPEFIAALLACGVTGRGAGRGLFEVATWNPRDWTHD
                     RHHTVDDRPYGGGPGMLMKVQPLRDAITAARAASPGRPRTIYLSPQGRRLDQAGVREL
                     AALPRLLLVCGRYEGIDERLIATEIDEEWSIGDYVLSGGELAAAVLIDALARLAPGVL
                     GNAASHEQDSFADGLLDCPHYTRPEEIAGLQVPAVLRSGDHAAIARWRRQQALGRTWL
                     RRRDLLAGRCLDRQDQALLDAFIRQHEERGSAAPGAADPASQGVGAAAPDPAEP"
     gene            3770..4120
                     /gene="rplS"
                     /locus_tag="PK144_14960"
     CDS             3770..4120
                     /gene="rplS"
                     /locus_tag="PK144_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNF67075.1"
                     /translation="MSNIIDQLNQEQIAGKTIPDFGPGDTVVVQVKVKEGNRERLQAF
                     EGVCIARRNRGLNSAFTVRKISHGEGVERVFQTYSPSIADISVKRRGDVRRAKLYYLR
                     GLEGKAARIKEKLS"
     gene            4330..>4973
                     /gene="cysQ"
                     /locus_tag="PK144_14965"
     CDS             4330..>4973
                     /gene="cysQ"
                     /locus_tag="PK144_14965"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNF67076.1"
                     /translation="MSQDFAQWLTPVRTIAAEAAARILEIYDTNFDVETKGDDSPLTA
                     ADRAAHECIVAGLTALTPDIPVLSEESRALPFEQRAAWSRYWLVDPLDGTKEFIKRNG
                     EFTVNIALIDGHRPVLGVVHVPVGNEVYFAALGHGSWHARAGAAPERIHVQIPCKPEV
                     RVVGSRSHGSDAMDGFINRLGDHAFVACGSSLKFCRVAEGRADVYPRLGLTSEW"
BASE COUNT          804 a         1622 c         1778 g          769 t
ORIGIN      
        1 cgagcagcgc gtaggcggtg atcgagatca ggatgtgcag ttccagccgc cactgcgcgg
       61 cctcggtgac cagtcgcgtc gacgggaacg ccagcgccag cacgacggtc gtcgccgtga
      121 acggcagcag cacgatgccg aggttctcga ccgggcgcgc cagcgaactt gcgagcagca
      181 gcagcgcgat cagccagccg gtgaacgaca gcgcgttgaa gaagccgagg ttcatgcctt
      241 cgggctgcac gacggtctgg ccgagcacga aggcgtgcag cacgatcgcg accagaccga
      301 gcgccagcgc cggtgcgcgc gccggctggt gcggctggtt gacgcgcgcc agccgcacgc
      361 cgagcagcgt gccggtggcg aggtagagca gggcggcgag ggcgccggtc aggatgctgg
      421 tcatgcgggc gtcacgggca ggtgagcaga accgccgcat gatcgcacac tggcgcgcgt
      481 gcctgacccg gcgcggtccg tcccctatac tgccgccctt ttctgcccgc tgccgcgagc
      541 ccgagtccga cgccatgttc gagaaccttt ccgagcgcct gctgcgcacc gtcaagcacc
      601 tgcgcggtca ggcgcgtctg accgaggaca acatccgcga cgcgctgcgc gaggtgcgca
      661 tggcgctgct cgaagccgac gtggcgctgc cggtggtgcg cagcttcatc gaacaggtgc
      721 gcgtgcgcgc ggtcggccag gaggtcatga ccgcgctgtc gcccgggcag gtgctggtca
      781 agatcgtcaa cgaggaactc gtgcggctga tgggcgaggc caacgaggcg ctggacctgc
      841 gcgcgcagcc gccggccgtg gtgctgatgg ccggcctgca gggctcgggc aagacgacca
      901 gcaccgccaa gctcgcgcgc ttcctgcgcg agcgcgaaaa gaagaaggtg ctggtggtca
      961 gctgcgacgt ctaccggccg gcggcgatcc agcagctggc cacgctcgcg aaggaagtcc
     1021 aggtcgagtg gtttgcgagc gagggcggcc agcagccggt ggcgatcgcg caggcggccc
     1081 ttgcgtacgc gaaacggcag ttcttcgatg tgctgatcgt cgataccgcc ggccgcctgc
     1141 acgtcgatgc ggcgatgatg gaggaaatcc aggcgctgca cgcggcggtg acgccggtcg
     1201 agacgctgtt cgtcgtcgat gcgatgaccg gtcaggacgc cgccaacacc gcgaaggcct
     1261 tccacgaggc gctgccgctg accggcgtcg tgctgaccaa gaccgacggt gacgcgcgcg
     1321 gcggtgcggc gctgtcgatc cgctacctga ccggcaagcc gatcaagttc ctcggtgtcg
     1381 gcgagaagac cgccgcgctc gaacccttcc atccggatcg cgtcgcctcg cgcatcctcg
     1441 gcatgggcga cgtgctcagc ctggtcgagg aggtcgagcg caaggtcgac aaggaaaagg
     1501 cgctgaagct cgccgagaag gtcaagtccg gcaagagctt cgacttcgag gacttccgcg
     1561 agcagatgga acagatgctg gcgatgggcg gcatggccgg gctgctcgac aagctgccgg
     1621 gcatggacaa ggtgccggac gatctgaaga agcaggccaa cgacaagcag gtgcgccggc
     1681 tgatcggcat cgtcaactcg atgacgccgc aggagcggcg ctttccggac ctgatcaagg
     1741 gctcgcgcaa gcgccgcatc gccgccggcg ccggcgtgca ggtgcaggaa atcaaccgcc
     1801 tgctcaagca gttcgaggaa atgcgccgga tgatgaagaa gatgtccggc ggcggcatga
     1861 tgaagctgat gcgggcgatg aagggcaagc tgccgatggg tggactcggg ggcctcggcg
     1921 gtcccggcgg cctgccgccg ttcaagtgag cgttcagatg aggcgatgcg ccgacccttg
     1981 agccatcggc gttttcgcgt acaatccgcg gttttctccg ggaccgcgcc tgcgggcgcg
     2041 gtcttttcgt ccccgggaca gacacggctc acaccggcac acccggtaca aactggatcg
     2101 caagaggatc gctatggtca ccatccgact ggcgcgcggc ggcgcgaaga agcgcccgtt
     2161 ctaccacgtc gtcgtcaccg acagccgcaa ccgccgcgac ggtcgctaca tcgagcgcct
     2221 cggtttcttc aatccgatcg cccgtggtgg cgaagtgcgc ctgaacctcg acgccgggcg
     2281 cgtgcagtac tggcagggca acggcgcgca ggtgtccgag cgcgtcgccg cgctgctgaa
     2341 ggaattcgac aaggcgcagg cggctgcggc ctgatccgca cgccgggcgc tgcatccgtg
     2401 agcatcgctg ctgccgacag ctgggtcgtg ctcgggcgcg tcgccggtct ctggggcgtg
     2461 cgcggcgggc tgaagatctg gtccgacacg cagccgcgcg ccgcgatctg ccgttaccgg
     2521 ccgctgtttc tcggtcgtga tggccagtgg cgcgaggtcg tgccggtgct cggtcgcgag
     2581 cagggcaagg gcatcgtcat gcagttcgac ggcgtcgagg atcgcgatgc cgctgcggca
     2641 ctgatcggct gcgagatcgc gatccgtcgc gaacagctgc cagccccggc tccgggcgag
     2701 tattactgga ccgatctggt cggcctgcgc gtcgagacgc tggatggcat cgaactcggc
     2761 cacgtcgatc acctgttcga gaccggctcg aacgacgtga tcgtcgtgcg cggcgagcgc
     2821 gagcgcctga tcccgttcct gcgcgggtcg gtgatccagg acatcgatct cgaagccggc
     2881 cggatgcgcg tggactggga tccggatttc tgatccggaa caagccgctg atcatccgtg
     2941 cgcatcgacg tcgtcacgct gtttccggag ttcatcgcgg cgctgctcgc ctgcggcgtg
     3001 accgggcgcg gggccgggcg cgggctgttc gaggtcgcga cctggaatcc gcgcgactgg
     3061 acgcacgacc gccaccacac ggtcgacgac cggccgtacg gcggcgggcc gggcatgctg
     3121 atgaaggtgc agccgctgcg cgatgcgatc acggcggcgc gggcggcgag ccccgggcgg
     3181 ccgcggacga tctacctgag tccgcagggc cggcggctgg accaggccgg cgtgcgcgaa
     3241 ctcgccgcgc tgccgcggct gctgctggtg tgcggacgct acgaaggcat cgacgagcgc
     3301 ctgatcgcga ccgagatcga cgaggaatgg tcgatcggcg actacgtgct gtccggcggc
     3361 gagctggcgg cggcggtgct gatcgacgcg ctggcgcggc tggcgccggg cgtgctcggc
     3421 aatgccgcct cgcacgagca ggattcgttc gcggacggcc tgctcgactg tccgcattac
     3481 acgcggcccg aggaaatcgc cgggttgcag gtgccggcgg tgctgcgctc gggcgatcac
     3541 gccgcgatcg cgcgctggcg ccggcagcag gcgctcggac gtacctggct gcgccggcgg
     3601 gacctgctgg ccggccggtg cctcgacagg caggaccagg cgctgctgga tgccttcatc
     3661 cggcagcacg aagaacgtgg atccgcggcg ccgggcgccg cggatccggc atcacagggt
     3721 gtcggcgctg cggctccgga cccggcggaa ccgtagggga gtgaccttca tgagcaacat
     3781 catcgatcag ctgaaccagg aacagatcgc cggcaagacc atcccggact tcggccccgg
     3841 cgacaccgtc gtcgtgcagg tcaaggtcaa ggaaggcaac cgcgagcgtc tgcaggcctt
     3901 cgagggcgtc tgcatcgccc gccgcaaccg cggcctgaac tcggcgttca ccgtgcgcaa
     3961 gatctcgcac ggcgaaggcg tcgagcgcgt gttccagacc tacagcccga gcatcgccga
     4021 catcagcgtc aagcgccgcg gcgacgtgcg tcgcgccaag ctctactacc tgcgcgggct
     4081 cgaaggcaag gcggcgcgca tcaaggaaaa gctgtcctga tcgcggcggt ccgcggattc
     4141 gcgtgatcca cgaagacggg cgtcacccgc aagggcgacg cccgtcttcg tgtcagtcgt
     4201 ttgcatcttc acgccgggca tcggtttttg tgtggctgtg gtttcatgct gcagtgcagt
     4261 aagctttgcc gtgtttccgc agcacgatgg tgcgcgcgcc agatttcgtc cctgtcgagg
     4321 aatcccttca tgtcccaaga cttcgcccag tggctgacgc ccgtgcgcac catcgccgcc
     4381 gaagccgctg cccgcatcct cgaaatctac gatacgaatt tcgatgtcga aaccaagggc
     4441 gacgacagcc cgctgaccgc ggccgatcgc gccgcgcacg aatgcatcgt tgccgggctg
     4501 acggcgctga cgccggacat cccggtgctg tccgaagaat ccagggcgct gccgttcgag
     4561 cagcgggccg cgtggtcgcg ctactggctg gtcgacccgc tcgatggcac caaggagttc
     4621 atcaagcgca acggtgagtt cacggtcaac atcgcgctga tcgacggtca ccggccggtg
     4681 ctcggcgtcg tgcatgtgcc ggtcggcaat gaggtgtact ttgccgcgct cggtcacggc
     4741 tcctggcatg ccagggctgg tgccgcgccc gagcgcatcc acgtgcagat tccctgcaag
     4801 ccggaagtgc gggtcgtcgg cagccgctcg cacggcagcg atgcgatgga tggcttcatc
     4861 aaccgtctcg gggatcatgc cttcgtcgcc tgtggcagtt cgctgaagtt ctgccgtgtc
     4921 gccgaaggcc gtgccgatgt ctatccgcgc ctgggcctga cttccgaatg gga
//
LOCUS       DAOQIE010000282         4967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9747_length_4967_cov_7.654520, whole genome shotgun sequence.
ACCESSION   DAOQIE010000282 DAOQIE010000000
VERSION     DAOQIE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9747_length_4967_cov_7.654520"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK144_14970"
     CDS             complement(<1..205)
                     /locus_tag="PK144_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNF67077.1"
                     /translation="MNPTAAPLRHDWTVAEALELFALPFADLLYRAQGVHRQHFDPNA
                     VQVSTLLSIKTGACPEDCAYCPQS"
     gene            complement(202..600)
                     /locus_tag="PK144_14975"
     CDS             complement(202..600)
                     /locus_tag="PK144_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67078.1"
                     /translation="MKTENTPVGKVLRASESRRAAGMLNIGNIVAILLPVPLLIFWFG
                     ASMLVYAMNRHHPDKRVGHYTQQAAYRLYGVTGFFTAAATFIPGGGWTWYIVAWVAAA
                     LVLIPWSVLDLRRIRREDWADIILPEPRTA"
     gene            732..1475
                     /locus_tag="PK144_14980"
     CDS             732..1475
                     /locus_tag="PK144_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNF67079.1"
                     /translation="MVYRWINLVQNRLLPAVCLLCGAPGEAPGRDLCAGCRRDLPLNA
                     HACPRCAEPLPASAPAGTVCGRCRRLPPPFAAAFAPYRYDWPLDRLIVELKFRRRLAV
                     ARVLGTLFAEALAVRLAAGAPRPQALVPVPLHPKRLAARGYNQAAELARPVARMLGLP
                     LLDAGRRVRDTAAQSALAFRERRRNVAGAFVLAQGVPHAHIALIDDVLTTGHTAGECA
                     RALQAGGVERVEVWTLARTPAPGKPGRTA"
     gene            complement(1492..2406)
                     /locus_tag="PK144_14985"
     CDS             complement(1492..2406)
                     /locus_tag="PK144_14985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNF67080.1"
                     /translation="MHTPDWDDYRYFLATAQTGSLSGAARELRTNQPTVGRRIAALEE
                     VLGVRLFHRHARGMSLTEAGARMLHSVQTMEEAALAAGRAVTGEDASLAGTVRISAPE
                     SIGSLFVAPRLGRLNGRYPAIQIVLQLTAHTADLLRNEADIAVRLFRPTAADLVARRV
                     GQVPFALYASPKYLNAAPALVAPADLAQHPQIGYDDEYVHLPEAEWQAGYARGARWSL
                     RSNSAFARAGGAAAGMGVALLPSVVGEIWPGLVRVLPNDAPEPREAWLVVHKDLRYVA
                     RIRVVLDFLAEIFEETRSPAAPQPARAV"
     gene            2821..>4967
                     /locus_tag="PK144_14990"
     CDS             2821..>4967
                     /locus_tag="PK144_14990"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNF67081.1"
                     /translation="MALEPRLVFDGAGAATAHAAGRDHADGADADGSDAGGAADAHDT
                     PPSPDTPAAAGSPAASGSAPSIALIDTRVAGYAAIAASLPADTHIVYVDGSRDGLAAL
                     DAAIRDAGAPVASVSIYAHGSESSITLGKRTLDAAALSGEAAGVLAAWREALSPDADL
                     LIYGCSVAAGSDGVGFVHALSELTGADVAASDDATGSAAAGGDWALEVASGAIEHASG
                     AVDGFSGLLLPTSITDSTPGTRTVAEDGTLTVTGLTLADGDDPAAMTLRVQTTGGSST
                     FALTGLTVTAGSNGSADVTLSGSRANLAAALASFTFSPAANQNSGISGYAPHIDFTAT
                     DVSNHDTSAGFTLNGLDVTAANDAPTLAVTPVTVAEGASSSFTAGNFNLVDVDNLPAQ
                     VIYKITALPAHGTLSLAGNPLVIGSSFDSTQAGNLVYTHDGTQVRASLYAGSEADSFT
                     IRVDDGAQGVIAAVTVPVVLTPVNQAPGISAGATVWEGQQNVPVTLTISDPDQASGLA
                     HTIRIDMLPLRGTLYYDGIAVTTGQSIADPTKLSYSHDGNDLNGGFPPNETFQISVTD
                     DGGGQGAGYEQSVTQTVTLTINRVNDDPRLLTNDPLTVNGASNPIGNTLLAVSDPDSF
                     AQNLTFTLTDTSGLAHGELQLNGVRLDAGASFTQLDIDNGALRYVWTNGTQADTFTDT
                     FKFTVKDGAITRLPNDFTQTREGGIYPNASDPNA"
BASE COUNT          746 a         1792 c         1771 g          658 t
ORIGIN      
        1 cgctctgcgg gcagtaggcg cagtcctcgg ggcaggcacc ggtcttgatc gacagcagcg
       61 tcgagacctg cacggcgttc gggtcgaagt gctggcgatg cacgccctgc gcgcggtaca
      121 gcagatccgc gaacggcagc gcgaacagtt cgagggcttc ggcgacggtc cagtcgtggc
      181 gcagcggggc ggcggtcggg ttcatgcagt gcggggctcc ggcaggatga tgtcggccca
      241 gtcctcgcgg cggatgcggc gcagatcgag cacggaccac gggatcagca ccagcgccgc
      301 ggccacccac gcgacgatgt accaggtcca gccgcccccc gggatgaaag tcgcggcggc
      361 ggtgaagaag ccggtgacgc cgtagagccg gtacgcggcc tgctgcgtgt agtggccgac
      421 gcgcttgtcg gggtggtggc ggttcatcgc gtagaccagc atcgaggcgc cgaaccagaa
      481 gatcagcagc ggcaccggca gcaggatcgc gacgatgttg ccgatgttga gcatgccggc
      541 cgcgcgccgc gattcgctgg cgcgcagcac ctttccgacg ggggtgtttt cggtcttcat
      601 ccggtttcgg cctggcgggg cggcagccgg ataccggtcg ggcgcagccg gaccggtgcg
      661 cggcgcgcgg ggacgggaat gcgccgcatg ctaacagccg gcgcccggct gtcaaccacg
      721 gagccggccg gatggtttac aggtggatca atcttgtaca gaaccggctg ctgccagcgg
      781 tctgtctgct ctgcggagcg ccgggcgagg cgccggggcg cgatctctgc gccggctgcc
      841 ggcgcgatct gccgctcaac gcccatgcct gtccgcgctg cgccgaaccg ctgccggcgt
      901 ccgcgccggc cggcacggtc tgcggccgct gccgccggct gccgccgccg ttcgcggccg
      961 ccttcgcccc gtaccgctac gactggccgc tggaccggct gatcgtcgag ctgaagttcc
     1021 gccggcggct ggcggtggca cgcgtgctcg gcacgctgtt cgccgaggcg ctggccgtgc
     1081 gcctcgccgc cggtgcgccg cgtccgcagg cgctggtgcc ggtgccgctg cacccgaagc
     1141 ggctggcggc gcgcggctac aaccaggcgg ccgaactggc gcggccggtg gcgcgcatgc
     1201 tcggcctgcc gctcctcgat gccggccggc gcgtgcgcga caccgccgcg cagagcgcgc
     1261 tggcgtttcg cgaacgccgg cgcaacgtcg ccggcgcctt cgtgctggcg cagggcgtgc
     1321 cgcacgcgca catcgcgctg atcgacgacg tgctgaccac cggccacacc gccggcgaat
     1381 gcgcacgggc gctgcaggcc ggcggcgtgg agcgggtcga ggtctggacg ctggcgcgca
     1441 cgccggcgcc ggggaagccc gggcgcacgg cgtgaacgga gggcgcccgg ctcagaccgc
     1501 ccgtgccggc tgcggcgcgg ccgggctgcg ggtttcctcg aagatctcgg cgaggaagtc
     1561 gagcacgacg cggatgcgcg cgacgtagcg caggtccttg tgcacgacca gccaggcctc
     1621 gcgcggctcg ggcgcgtcgt tcggcagcac gcgcaccagc cccggccaga tctcgccgac
     1681 cacgctcggc agcagcgcca cgcccatgcc ggccgccgcg ccgccggcgc gcgcgaaggc
     1741 gctgttcgag cgcagtgacc agcgcgcacc gcgcgcgtag ccggcctgcc actcggcttc
     1801 gggcagatgc acgtattcgt cgtcgtagcc gatctgcggg tgctgggcca gatcggccgg
     1861 tgccaccagc gccggcgcgg cgttcaggta cttcggcgag gcgtacagcg cgaacggcac
     1921 ctgaccgacg cggcgtgcga ccagatcggc cgcggtcgga cggaacaggc gcacggcgat
     1981 gtcggcctcg ttgcgcagca ggtcggcggt gtgcgccgtg agctgcagca cgatctggat
     2041 cgccggatag cggccgttca gccgcccgag gcgcggcgcg acgaacaggc tgccgatgct
     2101 ctcgggcgcc gagatgcgca cggtgccggc gaggctcgca tcctcgccgg tcacggcgcg
     2161 accggcggcg agcgcggctt cttccatcgt ctgcaccgaa tgcagcatgc gcgcgccggc
     2221 ctcggtcagg ctcatgccgc gcgcgtggcg atggaacagc cgcacgccga gcacttcctc
     2281 cagcgccgcg atgcgccgcc cgacggtcgg ctggttggtg cgcagctcgc gtgcggcacc
     2341 cgacaggctg ccggtctgcg cggtggcgag gaaatagcgg taatcgtccc agtcgggcgt
     2401 atgcatgggg ctctcccgga atattccggc cccccggggc cggtgcggcg ttcttctggt
     2461 gctggagacc ggtgatccgg accgctcatg cgcgccactg tagtcagcgc cggcgcggcg
     2521 ggcgaccgcg gccgacgcga cgatttcttg acgcaagcga ctaggcaagg cgtggttgcg
     2581 cgtcaagtga cgaaaaagca aactccgctc cttaccgccc accatgcacg tgcggcatcc
     2641 ctccgatccg atggactcac ggattcgatg gatggccagg ctttgcgtgc ctgtattcct
     2701 ggaggcggaa cagccggtgc gggaggcaca gccatgcgtg gacctcggga tttggtgtcg
     2761 ccagaacagg aaggtctccg atcccgggaa tcggccatcg ggcgcccgca ttcgctgcgc
     2821 atggccctgg agccgcggct ggtgttcgac ggcgccggcg ccgccacggc ccatgccgcc
     2881 gggcgcgatc acgccgatgg cgcagacgcc gacggcagcg atgccggcgg cgcggcggac
     2941 gcacacgaca cgcccccctc cccggacacc ccggccgcgg ccggcagtcc ggccgccagc
     3001 ggcagcgcgc cgtcgatcgc gctgatcgac acccgcgtcg ccggctacgc ggccatcgcc
     3061 gccagcctgc cggccgacac gcacatcgtc tacgtcgacg gcagccgcga cggcctcgcc
     3121 gcgctcgatg ccgcgatccg cgatgccgga gcgccggtcg cgagcgtctc gatctacgcg
     3181 cacggcagcg aatcctcgat cacgctcggc aagcgcacgc tcgatgccgc ggcgctctcc
     3241 ggcgaggccg ccggcgtgct cgccgcctgg cgcgaggcgc tgagccccga tgccgacctg
     3301 ctgatctacg gctgctcggt ggccgccggc agcgacggcg tcggcttcgt gcatgcgctt
     3361 tcggagctga ccggcgccga tgtcgccgcc agcgacgatg ccaccggcag cgccgcggcc
     3421 ggcggcgact gggcgctcga agtcgcgagc ggcgccatcg aacacgccag cggggccgtc
     3481 gacggcttca gcggcctgct gttgccgact tcgatcaccg acagcacgcc cggcacgcgc
     3541 acggtcgccg aggacggcac gctgaccgtc accggcctga cgctcgccga tggcgacgat
     3601 ccggcggcga tgacgctgcg cgtgcagacc accggcggca gcagcacgtt tgcactcacc
     3661 ggcctgacgg tgacggccgg cagcaacggc tcggccgatg tcacgctgtc cggctcgcgc
     3721 gcgaacctcg ccgcggcgct ggcgagcttc acgttcagcc ctgcggccaa ccagaacagc
     3781 ggcatcagcg gctacgcgcc gcacatcgac ttcacggcca ccgacgtgag caatcacgat
     3841 acgtcggcgg gcttcacgct gaacggcctc gatgtcaccg ccgccaacga cgcaccgacg
     3901 ctggccgtca cgccggtcac ggtcgccgag ggcgcgagca gcagcttcac ggccggcaac
     3961 ttcaacctcg tcgatgtcga caacctgccg gcgcaggtca tctacaagat caccgcgctg
     4021 ccggcgcacg gcacgctgtc gctcgccggc aatccgctgg tgatcgggtc gagcttcgac
     4081 agcacgcagg ccggcaacct cgtctacacg cacgacggca cgcaggtgcg cgccagcctg
     4141 tacgccggca gcgaggccga cagcttcacg atccgcgtcg acgatggcgc gcagggcgtc
     4201 atcgcagcgg tgacggtgcc ggtggtgctg acgccggtga accaggcgcc cggcatcagc
     4261 gccggcgcga cggtctggga aggccagcag aacgtgccgg tgacgctgac gatcagcgat
     4321 ccggatcagg cgagcggcct cgcgcacacg atccgcatcg acatgctgcc gctgcgcggc
     4381 acgctgtact acgacggcat cgcggtcacg accggccaga gcatcgccga cccgacgaag
     4441 ctcagctaca gccacgacgg caatgatctg aacggcggct tcccgccgaa cgagaccttc
     4501 cagatcagcg tcaccgacga cggcggtggt cagggcgccg gatacgaaca gtcggtgacg
     4561 cagaccgtca cgctgacgat caaccgcgtc aacgacgatc cgcggctgct gaccaatgac
     4621 ccgctgaccg tcaacggcgc cagcaatccg atcggcaaca cactgctcgc ggtcagcgat
     4681 ccggattcgt tcgcgcagaa cctgaccttc acgctgaccg acacctcggg gctggcgcac
     4741 ggcgaactgc agctgaacgg cgtgcgcctc gatgccggcg cgagcttcac gcagctcgac
     4801 atcgacaacg gcgcgctgcg ctacgtctgg accaacggca cgcaagccga caccttcacc
     4861 gataccttca agttcacggt gaaagacggc gcgatcacgc ggctgccgaa cgatttcacg
     4921 cagacgcgcg agggcggcat ctacccgaat gccagcgatc cgaacgc
//
LOCUS       DAOQIE010000283         4940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9825_length_4940_cov_7.195087, whole genome shotgun sequence.
ACCESSION   DAOQIE010000283 DAOQIE010000000
VERSION     DAOQIE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9825_length_4940_cov_7.195087"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..605)
                     /locus_tag="PK144_14995"
     CDS             complement(66..605)
                     /locus_tag="PK144_14995"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNF67082.1"
                     /translation="MSAVFNSPPAEARWYRQFWPWVLLGLPASAVTGCLITIWIATSN
                     PDGVVVDDYYKQGLAINTDQGRSVRASALGLHGRLQLDADSGRIGVDLAADAAGAQLP
                     PALTLRLLHATRSGIDVSLTLAGVAPGRYEAALPALAPGRWNLKAEAGDWRLDGSLNA
                     PHENAVALLPLVQMLPAER"
     gene            complement(625..2034)
                     /gene="ccoG"
                     /locus_tag="PK144_15000"
     CDS             complement(625..2034)
                     /gene="ccoG"
                     /locus_tag="PK144_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNF67083.1"
                     /translation="MTAETPSRPEGQDSGKSSFYAKTAKIYAREVKGRFATLRIYAVF
                     ALLGLYYGLPWLPWDGRQAVLFDLPARKFYIFGWVFWPQDFFFLALLLIIAALALFFF
                     TALAGRLWCGYACPQTVWTETFMWIEHWVEGDRNARMKLDKAPWSAGKFRKKLLKHSL
                     WALFALWTGFTFVGFFTPMHDLAARLWPFDWGPWETFWVFFYAFATWGNAGFMREQVC
                     LYMCPYARFQSAMFDKDTLVISYDAERGEPRGSRKRGTDPKAAGLGSCVDCTMCVQVC
                     PTGIDIRNGLQYQCIGCAACIDACDDIMDKMNYPRGLIRYTTEHSLMHDVPHVIRPRM
                     MVYGGLLLALTIGLLVAIALRVPLQLDVIRDRNALYRMNDEGLVENVYTLKIINMDER
                     EHRYRLGVSGVPGLKFTQDPGEIRVGSGEVFDLPTRVSADPEELKAASNEIRFDLVSV
                     DAPKLKTVTAGRFLGPARH"
     gene            complement(2205..2480)
                     /locus_tag="PK144_15005"
     CDS             complement(2205..2480)
                     /locus_tag="PK144_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67084.1"
                     /translation="MITRVQHIASLLWPSFLLAAAGTVILSTIFDPLAIAHCLGVTGI
                     ERTGTYSLGFFALWMMNTCSALLTLYFRQPVHTPVVCPVRDFDPDQN"
     gene            complement(2707..3594)
                     /gene="ccoP"
                     /locus_tag="PK144_15010"
     CDS             complement(2707..3594)
                     /gene="ccoP"
                     /locus_tag="PK144_15010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNF67085.1"
                     /translation="MSLTVSLYITLIVVGNTLAMWWLIRWTAKPRPNESVQGEVTGHT
                     WDGDLAEFNNPMPRWWLWMFYLTMVFTGVYVALYPGLGSVTGFLGWTQEGQWQSEMKE
                     AADKYDPIFKKYAGMDIAAVAKDPQALKMGGRLFATYCSSCHGSDAHGAVGFPNLADK
                     DWLYGGDPATIKTSIMNGRQGVMPAWSAILKEQGVEQVANYVLSLSGKSVDAAKAEAG
                     KALFLQNCAVCHGAEGKGNPVMGAPNLADNTWLYGGSLKAVEKTIADGRQGRMPAHAD
                     FLGNDKVHLLAAYVYSLSQ"
     gene            complement(3610..3780)
                     /locus_tag="PK144_15015"
     CDS             complement(3610..3780)
                     /locus_tag="PK144_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNF67086.1"
                     /translation="MTVVRSVFTVLMFVIFIGIVWWAWSRHNRKRFDDAASLPFADED
                     TARRTVEQEDRR"
     gene            complement(3799..4431)
                     /gene="ccoO"
                     /locus_tag="PK144_15020"
     CDS             complement(3799..4431)
                     /gene="ccoO"
                     /locus_tag="PK144_15020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010385442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNF67087.1"
                     /translation="MAHDNAPAVGHEKVERNIGLMVVLILIVVAFGGLAEIVPLFFLK
                     DTTEPVAGLKPLTALQLEGRDIYIREGCYTCHSQMVRPFRAETERYGHYSVAGEFVYD
                     HPFQWGSKRTGPDLARVGGRYSDDWHRTHLINPRDVVPESNMPGFPWLDTGKLDGELT
                     GKKMQTLRLVGVPYTDADIQGAKAAVQGKTEIEALIAYLQNLGTVISNKR"
     gene            complement(4447..>4940)
                     /locus_tag="PK144_15025"
     CDS             complement(4447..>4940)
                     /locus_tag="PK144_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF67088.1"
                     /translation="IVSLSFYGMSTFEGPMMSIKTVNALSHYTDWTIGHVHSGALGWV
                     ALISIGCVYALIPQLFNRRIYSTKLIEVHFWTGTIGIVLYIASMWIAGVMQGLMWRAV
                     NEDGTLTYSFVEALVRMYPYLTVRFLGGLLFLVGMFVMAWNVYKTLQGDEQPVSAPAP
                     QAA"
BASE COUNT          836 a         1606 c         1694 g          804 t
ORIGIN      
        1 atcgcgataa aaaagcccgg gcatccgccc gggctttttt tgtggctgcg gcttctgcgg
       61 gagcttcagc gctcggccgg cagcatctgc accagcggca gcagcgccac ggcgttctcg
      121 tgcggggcgt tcaggctgcc gtcgagccgc cagtccccgg cttcggcctt caggttccag
      181 cgcccgggcg cgagcgccgg cagcgccgcc tcgtagcgcc ccggcgcgac gccggccagc
      241 gtcagcgaca cgtcgatgcc gctgcgcgtc gcgtgcagca ggcgcagcgt cagcgccggc
      301 ggcagctgcg cgccggcggc atcggcggcg aggtcgacgc cgatccggcc gctgtcggca
      361 tcgagctgca ggcggccgtg caggccgagc gccgaggcgc gcacgctgcg gccctgatcg
      421 gtgttgatcg ccagcccctg cttgtaatag tcgtcgacga cgacgccatc ggggttgctc
      481 gtcgcgatcc agatcgtgat caggcagccg gtgacggcac tcgccggcag gccgagcagc
      541 acccagggcc agaactgccg gtaccagcgc gcttcggcgg gcggggagtt gaaaacggcg
      601 gacatcgggg ggagcctccg gcggtcagtg gcgggccggg ccgaggaagc ggccggcggt
      661 tacggtcttg agcttcggcg catcgaccga gacgaggtcg aagcggattt cattcgaggc
      721 ggccttcagt tcctcgggat cggcgctgac gcgggtcggc agatcgaaga cctcgccgct
      781 gccgacgcgg atctcgcccg ggtcctgcgt gaacttcagg ccgggcacgc cgctgacgcc
      841 gaggcggtag cggtgctcgc gctcgtccat gttgatgatc ttcagcgtgt agacgttttc
      901 gaccaggccc tcgtcgttca tgcggtacag cgcgttgcgg tcgcggatca cgtcgagctg
      961 cagcggcacg cgcagcgcga tcgccaccag caggccgatg gtcagcgcca gcagcaggcc
     1021 gccgtagacc atcatgcgcg ggcggatcac gtgcggcacg tcgtgcatca ggctgtgctc
     1081 ggtcgtgtag cggatcagcc cgcgcgggta gttcatcttg tccatgatgt cgtcgcaggc
     1141 atcgatgcag gctgcgcagc cgatgcactg gtactgcagg ccgttgcgga tgtcgatgcc
     1201 ggtcgggcag acctgcacgc acatcgtgca gtcgacgcag gagccgaggc cggccgcctt
     1261 cgggtcggtg ccgcgcttgc gcgagccgcg cggctcgccg cgctcggcgt cgtaggagat
     1321 gaccagcgtg tccttgtcga acatcgcgct ctggaagcgc gcatacggac acatgtacag
     1381 gcagacctgc tcgcgcatga agccggcgtt gccccaggtc gcgaacgcgt agaagaacac
     1441 ccagaaggtt tcccacggcc cccagtcgaa cggccagagg cgggcggcaa gatcgtgcat
     1501 cggcgtgaag aagccgacga aggtgaagcc ggtccacagc gcgaacagcg cccagagcga
     1561 gtgcttcagg agcttcttgc ggaacttgcc ggcgctccac ggcgccttgt cgagcttcat
     1621 gcgcgcattg cggtcgcctt cgacccagtg ctcgatccac atgaaggttt cggtccagac
     1681 cgtctgcggg caggcgtagc cgcaccacag ccgcccggcg agcgccgtga agaagaacag
     1741 cgccagcgcc gcgatgatca ggagcagcgc caggaagaag aagtcctgcg gccagaacac
     1801 ccagccgaag atgtagaact tgcgcgccgg caggtcgaag agcaccgcct gacgaccatc
     1861 ccagggcagc cagggcagcc cgtaatagag gccgagcagc gcgaacaccg catagatgcg
     1921 cagcgtcgcg aagcggccct tgacctcacg cgcgtagatc ttcgcagtct tggcgtagaa
     1981 gctgcttttg ccggaatcct ggccctcggg ccggctcggt gtttccgccg tcatgcaaac
     2041 gtcctcgaca tcggcgaccg gcgccggggg cggcgcggtt catcaggcac gcatgatgct
     2101 ttcttcagca cggcgccgca ttgcgaaaaa tcacgaatcg tcgtgcaaac acaggcttgc
     2161 gcgcagcctc gtggaatgcc ggcagatgcc ggaccgcagc cggctcagtt ctggtccgga
     2221 tcgaagtcgc gtaccgggca gaccaccggc gtgtgcaccg gctggcggaa gtacagcgtc
     2281 agcagcgccg agcaggtgtt catcatccac agcgcgaaga agccgagcga gtacgtgccg
     2341 gtgcgctcga tgccggtgac gccgaggcag tgcgcgatcg ccagcggatc gaagatcgtc
     2401 gacaggatca cggtgccggc ggccgcgagc aggaacgacg gccacagtaa tgaagcgatg
     2461 tgctggactc gggtgatcac gatgcttgcc tccggtgcct gacgggtgcg gacgcgccga
     2521 tgcgtgcggc gcggtctcgg atgcgggtgg attccgccgc tgcttaccgt agtcgctggc
     2581 tgcgccggcg gcagtgatga agcgcagtcg gccgccgcca ccggcctcgc ccgaaaaaaa
     2641 acgccgctcc ccggggggag cggcggcgtg cggctgcggc ccggcaccag tggccggaaa
     2701 ccgcgatcac tgcgacaggc tgtagacgta ggcggccagc aggtggacct tgtcgttgcc
     2761 gaggaagtcc gcgtgcgccg gcatgcggcc ctggcgaccg tcggcgatcg tcttctcgac
     2821 ggccttcagc gaaccgccgt acagccaggt gttgtcggcc aggttcgggg cgcccatcac
     2881 cgggttgccc ttgccttccg caccgtggca gacggcgcag ttctgcagga acagcgcctt
     2941 gccggcctcg gccttggcgg cgtcgaccga cttgccggac aggctcagca cgtagttggc
     3001 gacctgctcg acgccctgct ccttcaggat cgcgctccag gccggcatca cgccctggcg
     3061 accgttcatg atcgaggtct tgatcgtggc cgggtccccg ccatacagcc agtccttgtc
     3121 ggcgaggttc gggaagccga ccgcaccgtg ggcgtcggag ccgtggcagc tggagcagta
     3181 ggtcgcgaac agccggccac ccatcttcag tgcctgcggg tccttggcga cggcggcgat
     3241 gtccatgccg gcgtacttct tgaagatcgg gtcgtacttg tcggcggctt ccttcatctc
     3301 ggactgccac tggccttcct gcgtccagcc gaggaagccg gtgacgctgc cgaggcccgg
     3361 atacagggcc acgtagacgc cggtgaagac catggtcagg tagaacatcc acagccacca
     3421 gcgcggcatc ggattgttga actcggccag gtcgccatcc caggtgtgac ccgtgacctc
     3481 accctgcacc gactcgttcg gccggggctt ggcggtccag cggatcagcc accacatggc
     3541 cagcgtgttg ccgaccacga tcagggtgat gtaaaggctg acggtcagac tcatcttggc
     3601 atctctcctt cagcgccggt cttcttgctc gaccgtcctg cgcgcggtgt cctcgtcggc
     3661 gaagggcagg ctggccgcgt cgtcgaaacg cttgcggttg tgccggctcc acgcccacca
     3721 gacgatgccg atgaagatca cgaacatcag caccgtgaac acgctgcgca cgacggtcat
     3781 gaagaagtcc acgggcgctt acctcttgtt gctgatcacg gtgccgaggt tctgcagata
     3841 cgcgatcagc gcctcgatct cggtcttgcc ctgaaccgcc gccttcgcac cctggatgtc
     3901 ggcgtccgtg tacggcacac cgaccaggcg cagcgtctgc atcttcttgc cggtcagttc
     3961 gccgtcgagc ttgccggtat ccagccacgg gaagccgggc atgttggatt ccggcaccac
     4021 gtcgcgcgga ttgatcaggt gcgtgcgatg ccaatcatcg gagtagcgac cgccgacacg
     4081 cgccagatcc ggaccggtgc gcttggagcc ccactggaac gggtggtcgt agacgaactc
     4141 gccggccacc gagtagtggc cgtagcgctc ggtctcggcg cggaacggac gcaccatctg
     4201 cgagtggcag gtgtagcagc cttcgcggat gtagatgtcg cggccttcga gctgcagcgc
     4261 ggtcagcggc ttgaggccgg cgaccggctc ggtcgtgtcc ttcaggaaga acagcggcac
     4321 gatctcggcg aggccgccga aggcaacgac gatgaggatc agcacgacca tcaggccgat
     4381 gttgcgctcg accttctcgt ggccgaccgc cggggcattg tcatgagcca tgttggtatg
     4441 tctcccttaa gcggcctgcg gcgccggcgc ggacaccggc tgttcgtcgc cctgcagcgt
     4501 cttgtagacg ttccaggcca tcacgaacat gccgacgagg aacagcagac cgccgaggaa
     4561 gcggaccgtc aggtacgggt acatgcgcac gagggcttcg acgaagctgt aggtcagcgt
     4621 gccgtcctcg ttgaccgcac gccacatcag gccctgcatg acgccggcga tccacatcga
     4681 ggcgatgtag agcacgatgc cgatcgtgcc ggtccagaag tggacctcga tcagcttggt
     4741 gctgtagatg cggcggttga acagctgcgg gatcagcgca tagacgcagc cgatcgagat
     4801 cagcgccacc cagccgaggg cgccggagtg cacgtggccg atggtccagt cggtgtagtg
     4861 cgacagcgcg ttgacggtct tgatcgacat catcggacct tcgaaggtcg acatgccgta
     4921 gaacgacagc gagacgatca
//
LOCUS       DAOQIE010000284         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_9834_length_4938_cov_5.710424, whole genome shotgun sequence.
ACCESSION   DAOQIE010000284 DAOQIE010000000
VERSION     DAOQIE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9834_length_4938_cov_5.710424"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..570)
                     /locus_tag="PK144_15030"
     CDS             complement(160..570)
                     /locus_tag="PK144_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNF67089.1"
                     /translation="MSPSGWHILGAGAIGGLYATGLAAAGVPVTVLTRDTDSAAVLMR
                     EGLTVEEPDGHELHARPLAEPVVAGGASIRRLLVVTKAQDTVAALELIAHRLGPVAVH
                     VAAERAVVVGLAGVAALGGQKRPGGEDARPQDLA"
     gene            complement(672..1856)
                     /gene="dapE"
                     /locus_tag="PK144_15035"
     CDS             complement(672..1856)
                     /gene="dapE"
                     /locus_tag="PK144_15035"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNF67090.1"
                     /translation="MSQAPASTTLALARELIARRSVTPADAGCQALMMERLAALGFEC
                     QRLRFGEVENFWARRGRAAPLFAFAGHTDVVPTGPLERWASDPFTPELRDGVLYGRGA
                     ADMKGSLAAMVTACERFIDAHPQHAGSIGFLITSDEEGPATDGTVRVVDWLGARGEHI
                     DWCLVGEPSSAHTLGDTIKNGRRGSLHGHLTVHGVQGHVAYPHLVHNPIHALGPIIAV
                     LADEIWDHGNEFFPPTSFQISNVHGGAGATNVVPGTAELRFNFRFSTESTVEQLQERT
                     RLLVEMALLNEEVKAGWRFDATLEWELSGLPFLTEPGALVDAAVEAIYAETGTEAQLS
                     TSGGTSDGRFIAPTGAQVLELGPLNATIHKIDECVAADDLDALSRIYEGILQRLLARA
                     AG"
     gene            1947..2351
                     /locus_tag="PK144_15040"
     CDS             1947..2351
                     /locus_tag="PK144_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67091.1"
                     /translation="MKHQKIILRLSLAALLLAVAGAAAARDYHHPPGYGWPVAPRGPT
                     STTLSGTLPQGRPMPRPGPYYTLPSPGGRTEIHDFSGQRYGTLERETGSRPPVVYAPD
                     GRRRYETDRDGTLRDPYGRRIEVLKPLPDGDW"
     gene            2399..3058
                     /locus_tag="PK144_15045"
     CDS             2399..3058
                     /locus_tag="PK144_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF67092.1"
                     /translation="MQRIAVYCGSASGSDPRFAEAATAFGALLAARGLELVFGGGHVG
                     LMGRVADAVLAGGGRAIGVMPDLLAEREIAHRGLTELQIVPDLQQRKRRMLELADACI
                     ALPGGIGTFDEWFEALTLGCLELHDRPCALLNLHGYHDPLLALLDHAVAQGFMRPQQR
                     AAVLVDDDGARLLDACAARRATQAFNGDPIGQDAEQRTKKTRSYRQGEGGDRVIGGSD
                     I"
     gene            3468..4592
                     /locus_tag="PK144_15050"
     CDS             3468..4592
                     /locus_tag="PK144_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67093.1"
                     /translation="MPSLVCLSGLAMPAPLLACGPWRLLRFLQDQPLIWPSLLSADEI
                     AARLEAGPCSSRTGPEWTLIVPADRPLGADLGLEARQRLVRFSERLSCATLAQLSLET
                     HTDFRCPDPLRIRMQHWQSAQSVPAAVPLATGMPAPAANDHRRSLAFDVDLLQALIEL
                     DRMPAGQTAAIDKALQAYGLALAQAERLIPVTALMLLGAALRSLLACEDYDATLRDRL
                     LAEIALLSGPDTGERAAGSSDPATGLLPGRLLREWLDDLFGCLGRSGQGDPLRGGNWA
                     ARSHLRLLGFLFPLLLKARLARLGLYRISGTDIALVGCFERLTAAEPHLLGARRAGLQ
                     APLATAASIVQGALTPDPQVAAFEDALAWCINRNLLMPDP"
     gene            4753..>4938
                     /locus_tag="PK144_15055"
     CDS             4753..>4938
                     /locus_tag="PK144_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67094.1"
                     /translation="MNGYFSKRDVQARMLKELRDARARGASGAEQLAILHRWAPFLAD
                     FDSDESDSDDSDGDLRDE"
BASE COUNT          759 a         1687 c         1731 g          761 t
ORIGIN      
        1 ctcatcgacg cccacgttca cctgttccac tcgggcacca tctcctgggt gggcgacagc
       61 ctcgaggcga acctgtccgc cgagctcgcc tggggcgtgg tgggcgtggc cgatctgggc
      121 gcgcccgccg aggtctttca gctccgcgac cgcgtccact caggcgagat cctggggccg
      181 cgcatcttcg ccaccgggcc gtttttgacc gccgagggca gccacccctg cgagacctac
      241 aacgaccgcg cgctctgccg ctacgtggac ggcgacgggg ccgaggcggt gggcgatcag
      301 ttcgagcgcg gcgaccgtgt cctgcgcctt ggtcaccacc agcagccggc ggatgctcgc
      361 gccgccggcg acgaccggct cggcgagcgg gcgggcgtgc agctcgtggc cgtcgggctc
      421 ctcgaccgtc aggccctcgc gcatcagcac cgcggcactg tcggtgtcgc gcgtcagcac
      481 cgtcaccggc acgccggcag cggcgagccc ggtcgcgtac agcccgccaa tggcgccggc
      541 gccgaggatg tgccagccgg aaggtgacat cgtgttacgg tccccctgcg caaagagcgg
      601 cagggtagcg gtcaggccgg ggcttgtgaa ccggcttgtg cggggctgcc gctcccggcg
      661 ggcggggtgt ctcagccggc ggcgcgcgcc agcaggcgct gcaggatgcc ttcgtagatg
      721 cgcgacagtg catcgagatc atcggcggcg acgcattcgt cgatcttgtg gatcgtcgcg
      781 ttcagcggcc cgagttcgag cacctgcgca ccggtcgggg cgatgaagcg gccgtccgag
      841 gtgccgccgc tggtcgagag ctgcgcctcg gtgccggtct cggcgtagat cgcctcgacg
      901 gcggcatcga ccagcgcgcc cggctcggtc aggaagggca ggccggagag ttcccattcg
      961 agcgtcgcat cgaagcgcca gccggccttg acctcctcgt tcagcagcgc catctcgacc
     1021 agcagccgcg tgcgctcctg cagctgctcg accgtcgatt cggtcgagaa gcggaagttg
     1081 aagcgcagct ccgccgtgcc cggcacgacg ttggtggcgc cggcgccgcc gtggacgttg
     1141 gagatctgga acgaggtcgg cgggaagaac tcgttgccgt ggtcccagat ttcgtcggca
     1201 agcacggcga tgatcggtcc gagcgcgtgg atcgggttgt gcaccagatg cggataggcg
     1261 acatggccct gcacgccgtg cacggtcaga tgcccgtgca gcgagccgcg ccggccgttc
     1321 ttgatcgtgt cgccgagcgt gtgcgccgag gacggctcgc cgaccagaca ccagtcgatg
     1381 tgctcgccgc gcgcgcccag ccagtcgacg acgcgcaccg tgccgtcggt ggccgggcct
     1441 tcctcgtcgc tggtgatcag gaagccgatc gagccggcgt gctgcggatg tgcatcgatg
     1501 aagcgctcgc aggccgtgac catcgctgca aggctgccct tcatgtcggc ggcgccgcgc
     1561 ccgtagagca cgccgtcgcg caactcgggc gtgaacggat cggaggccca gcgttcgagc
     1621 gggccggtcg gcaccacgtc ggtatggccg gcgaaggcga acagcggcgc ggcgcgaccg
     1681 cggcgtgccc agaagttctc gacttcgccg aagcgcagcc gctggcactc gaaaccgagc
     1741 gcggcgaggc gttccatcat cagcgcctga cagccggcat ccgcgggcgt gaccgaacgg
     1801 cgggcgatca gctcgcgcgc gagggcgagc gtggtcgagg cgggggcttg ggacattccg
     1861 gactccggcg ggtcgaaaag cggcgtagtg tacgacgtgc tgccggccac ggccgcgggg
     1921 ctgcgaccgg cggggagggc aagatcatga agcaccagaa gatcatcctg cgcctgagcc
     1981 tcgccgcgct gctgctggcg gtcgccggcg ccgcggccgc ccgcgattac caccatccgc
     2041 ccggctacgg ctggccggtc gcaccgcgcg gcccgacctc gacgacgctg agcggcacgc
     2101 tgccgcaggg ccggccgatg ccgcggcccg ggccgtacta cacgctgccc tcaccgggcg
     2161 ggcgcaccga aatccatgat ttcagcggcc agcgctacgg cacgctcgaa cgcgaaaccg
     2221 gcagccgccc gccggtcgtc tacgccccgg acgggcggcg gcgctacgag accgatcgcg
     2281 acggcacgct gcgcgacccc tacggccggc gcatcgaggt gctgaaaccg ctgccggacg
     2341 gcgactggta gcgagtcctg cgctacggtg gctgccccgg aatcgaggag gccacgtcat
     2401 gcagcgcatc gccgtctatt gcggttccgc cagcggcagc gatccgcgtt tcgccgaagc
     2461 cgcgaccgcc ttcggtgcgc tgctcgcggc gcgcgggctg gagctcgtgt tcggcggcgg
     2521 ccacgtcggg ctgatggggc gggttgccga tgccgtgctc gccggcggcg ggcgcgcgat
     2581 cggcgtgatg ccggatttgc tggccgagcg cgagatcgca caccgcggtc tcaccgaact
     2641 gcagatcgtg cccgacctgc agcagcgcaa gcgccgcatg ctggaactcg ccgatgcctg
     2701 catcgcgctg cccggcggca tcggcacttt cgatgaatgg ttcgaggcgc tgacgctcgg
     2761 ctgtctcgaa ctgcacgacc ggccctgcgc gctgctgaac ctgcacggct atcacgatcc
     2821 gctgctggcg ctgctcgatc acgccgtcgc gcagggcttc atgcgtccgc agcagcgtgc
     2881 ggcggtgctc gtcgatgacg atggcgctcg gctgctcgac gcctgcgcgg cccggcgggc
     2941 gacgcaggcg ttcaacggcg atcccatcgg gcaggacgcg gagcagcgaa cgaaaaaaac
     3001 gcggtcgtac cgtcaggggg aaggcggcga ccgcgtaatt ggggggagcg acatctgaat
     3061 cgagggatgt cgcgacgcag aataccctag tgcagcgctc tggtatcagt cggacccgac
     3121 tttttttacc ggtgcaacag gggtgtcagc tatgcttaca tatccgggtc aagcgaccgt
     3181 ttcgacggat gtcgctatat acggcgactg acgtattacc acagccacca caatgtattt
     3241 tcatgagccc gtgctgtgca ggcgcagcat gacggccggt acggatcgaa gccgatgtaa
     3301 ttccggcctg ggcaaagtcc aggtcttcaa tcaagtcccg accgatggat cgacaaggca
     3361 cgctggcgtt gatccgtgcg tagaccggcc tgcgggtggt cgaacggacg tggccgacgc
     3421 ttacggttca cgtccaaccc ggcccgcgtg ccgcaagtcg accacccatg ccttctctcg
     3481 tctgcctgtc cggactcgcc atgccggcgc cgctgctggc ctgtggcccg tggcgactgc
     3541 tgcgcttcct gcaagaccag ccgctgatct ggccctcgct gctgagcgcc gacgagatcg
     3601 ccgcccgcct cgaagccgga ccgtgcagca gccgcaccgg cccggagtgg acgctgatcg
     3661 tgcccgccga ccggccgctc ggtgccgatc tggggctgga ggcgcgccag cgcctcgtgc
     3721 gcttcagcga gcggctgagc tgcgcgacgc tggcgcagct gagcctcgaa acacacaccg
     3781 acttccgctg cccggacccg ctgcgcatcc gcatgcagca ctggcaatcg gcgcagagcg
     3841 tccctgccgc cgtgccgctg gcaacgggga tgccggcacc ggcggcaaac gaccaccgac
     3901 gcagtctggc cttcgatgtt gacctgctgc aggcgctgat cgaactcgac cggatgccgg
     3961 ccgggcagac cgccgccatc gacaaggcgc tgcaggccta cgggctggcg ctggcacagg
     4021 ccgagcggct gattccggtg actgcactga tgctgctcgg tgctgcgttg cgcagcctgc
     4081 tcgcctgcga ggactacgac gcgacgctgc gcgatcggct gctggccgag atcgccttgc
     4141 tgtcaggacc cgataccggc gaacgggcag ccggcagcag cgaccctgcc accggcctgc
     4201 tgcccggtcg tttgctgcgg gaatggctgg atgatctgtt cggctgcctg ggccgcagcg
     4261 gtcaggggga tccgctgcgc ggcggcaact gggcggcgcg cagtcacctg cgcctcctcg
     4321 gtttcctgtt cccgctgctg ctgaaggccc ggctcgcgcg gctgggcctg taccggatca
     4381 gcggcaccga catcgccctg gtcgggtgtt tcgagcgcct gacggcagcc gaacctcacc
     4441 tgctcggcgc ccggcgcgcg ggtctgcagg caccgctggc gaccgcggcc agcatcgtgc
     4501 agggcgccct gactcccgac ccgcaggtcg cggccttcga ggatgcgctg gcctggtgca
     4561 tcaaccgcaa cctgctgatg ccggatccct gagccgatcc gggcttgcat ccgatcagca
     4621 tcggatcagg gcctgatcaa ttgaattgat tgaaaaatta gattgatcct gattcaggat
     4681 aaggaaactg cgtccggaaa gggctatata gcactgcaag aacagcaaca aatgtcgcaa
     4741 tggaggcgag ccatgaacgg ctacttttcc aaacgcgatg tgcaagcccg catgctcaag
     4801 gaactgcgcg atgcccgtgc ccggggagcc agtggcgccg agcagctcgc aatcctgcac
     4861 agatgggcac cgtttctggc ggacttcgac agcgacgagt ctgacagcga cgattccgac
     4921 ggcgatctgc gggacgaa
//
LOCUS       DAOQIE010000285         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10003_length_4879_cov_7.445688, whole genome shotgun sequence.
ACCESSION   DAOQIE010000285 DAOQIE010000000
VERSION     DAOQIE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10003_length_4879_cov_7.445688"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..173)
                     /locus_tag="PK144_15060"
                     /pseudo
     CDS             complement(60..173)
                     /locus_tag="PK144_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017035701.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
     gene            complement(181..633)
                     /gene="aroQ"
                     /locus_tag="PK144_15065"
     CDS             complement(181..633)
                     /gene="aroQ"
                     /locus_tag="PK144_15065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNF67095.1"
                     /translation="MAQLLVLNGPNLNLLGTREPATYGAQTLADIRQNCEALAAAQGH
                     ALDFFQSNAEHALIERIHAARTDGTDFIVINPAAYTHTSVALRDALLAVAIPFIEVHL
                     SNVHAREPFRHHSYFSDVALGVICGLGAQGYELALAAADRHLAARKPA"
     gene            complement(836..2713)
                     /gene="dsbD"
                     /locus_tag="PK144_15070"
     CDS             complement(836..2713)
                     /gene="dsbD"
                     /locus_tag="PK144_15070"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNF67096.1"
                     /translation="MSRFAVLLRRWLPVACMLLVAGLPPAMAIDLFGKKPSADDLLDA
                     EQAFFVEAPAVEPGAVRLRWTIADGYYLYRARLKVEPEGALPPGITIAGVDTPAGEIK
                     DDPSFGRTEVWHEAAIATVRLTRSGAGLGAQPLTLRAEWQGCNEPVGVCYPPQSKTWT
                     LELPAATATPMSATAAAAPAGPGAAPAATAPAPEPEQDRMARMLRNERFLALPAFFGF
                     GLLLSFTPCVFPMVPILSGLIVGRGRHTTRGHAFGLSVVYVLAMASTYTVAGVVAASF
                     GENLQAAFQHPGVLIAFALLFVALALSMFGLYQLQLPGALQARLSEISGHQRGGDYAG
                     VAIMGLLSALIVGPCVAPPLIGALAVIASTGDRLLGGAALFAMSLGMGVPLLAIGASA
                     GHFLPRAGHWMDKVKAVFGVALLAVALVMLDRVLPAALIMLAWAVLLVVSATYMGALQ
                     PIPQGAPWWRTLIKGLGLVLLMQGALLLIGVAAGARDPLRPLAGIVAAGGNSEAAHGL
                     AFRRIVSLAELDAEIAAAGGRPVLLDFYADWCVACKELERDTFPDPQVRTRLAGFVRL
                     QADVTANSADDRALLRRYGLVGPPAILIFGADGRERTELRTVGFVGPEPFAELLGRVG
                     S"
     gene            complement(2713..3048)
                     /gene="cutA"
                     /locus_tag="PK144_15075"
     CDS             complement(2713..3048)
                     /gene="cutA"
                     /locus_tag="PK144_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNF67097.1"
                     /translation="MDTPCQLVLCTCPDADSAGHIATVLVGEGLAACANLVPGVVSVY
                     RWQGAVQRDAEVLMLLKTRADAFEALAARLRELHPYTVPEIIAVGIEAGSPAYIGWLL
                     AETRAGGPA"
     gene            3206..3655
                     /locus_tag="PK144_15080"
     CDS             3206..3655
                     /locus_tag="PK144_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNF67098.1"
                     /translation="MKLPAVLFVLFVALPIVEIYVLVRVGSVIGALPTIALVIGLSAF
                     GAWLVRHEGLGVLQRVRTALDAGQLPARELLDGALILIGGVLLLTPGFVTDVFGLCCL
                     LPPVRAQLIRLLARHAIVAAGRRGPPGGGAPGARGPQTLEGEYRRED"
     gene            3863..4153
                     /gene="groES"
                     /locus_tag="PK144_15085"
     CDS             3863..4153
                     /gene="groES"
                     /locus_tag="PK144_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNF67099.1"
                     /translation="MSLRPLHDRVIVKRVEEETKSAGGIVLPGNAAEKPSRGTVLAIG
                     RGKILENGDVRPLDVKVGDHVLFGKYSGSEVKIDGDEVLVMREDEIMAVIEK"
     gene            4256..>4879
                     /locus_tag="PK144_15090"
     CDS             4256..>4879
                     /locus_tag="PK144_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNF67100.1"
                     /translation="MAAKDVKFGDDARSRMVRGINILANAVKVTLGPKGRNVVLDKAF
                     GAPTVTKDGVSVAKEIELSDKFENMGAQMVKEVSSKTSDVAGDGTTTATVLAQAIVRE
                     GMKSVSAGMNPMDLKRGIDKAVQAAVGELAKLSKPCADDKAIAQVGAISANGDEQIGR
                     IIADAMAKVGKEGVITVEDGSGLENQLDVVEGMQFDRGYLSPYFINNQ"
BASE COUNT          770 a         1674 c         1741 g          694 t
ORIGIN      
        1 ggcctggacc ggggctgaac gggttaggaa ccggcgccga gctgttcggc gcgggcgctc
       61 gcggccggat ggcgcgagat gcgcacggcc tcctcgcctt ccttgatctc gatttcggcg
      121 atgccggatt cctccagcag ttcgatgagc ttcttgatct tgcggatgtc catggatgtc
      181 ctatgccggc ttgcgggctg cgaggtgacg gtccgccgct gccagggcga gttcgtagcc
      241 ctgggcgccc agtccgcaga tcacgccgag cgccacgtcg gagaaatacg aatggtggcg
      301 gaagggttcg cgggcgtgga cgttggagag gtggacctcg atgaacggaa tcgcgacggc
      361 cagcagcgcg tcgcgcagcg ccacgctggt gtgcgtgtac gcggcgggat tgatgacgat
      421 gaagtcggtg ccgtcggtgc gcgcggcgtg gatgcgctcg atcagcgcgt gttcggcgtt
      481 gctctggaag aagtcgagcg catgcccctg cgcggcggcg agggcttcgc agttctggcg
      541 gatgtcggcc agcgtctgcg cgccgtaggt cgcgggttcg cgggtgccga gcaggttcag
      601 gttggggccg ttcagcacca gcagctgcgc catcgggaag ggactctcgc cagggagggc
      661 ggcgcgctcg ggcgcccgga acggggaagg cgcgcgattc tgccgtaacg atccggcact
      721 gtccatcggg cggatgccgg gctttgcgca tgacttcgcg agcaacgtcg ccgaacgcgc
      781 gggaatcgcg cgtgacaatt ccttcatgga gccggtgcgg cgggcggccg cggactcagc
      841 tgccgacgcg cccgagcagt tcggcaaacg gctccgggcc gacgaaaccg accgtgcgca
      901 gttcggtgcg ctcgcggccg tcggcgccga agatcaggat cgccggcggc ccgaccagac
      961 catagcggcg cagcagcgcg cggtcatcgg cggagttggc ggtgacatcg gcctgcaggc
     1021 gcacgaaccc ggcgagccgc gtgcgcacct gcgggtccgg gaaggtgtcg cgctccagtt
     1081 ccttgcaggc cacgcaccag tcggcataga aatcgagcag caccggccgg ccgccggcgg
     1141 cggcgatctc ggcatcgagt tcggcgaggc tgacgatgcg ccggaaggcc aggccgtgcg
     1201 cggcttcgga attgccgccg gccgcgacga tgccggccag cggccgcagc ggatcacgcg
     1261 caccggccgc cacgccgatc agcagcagcg cgccctgcat cagcagcacg aggccgaggc
     1321 ccttgatcag cgtgcgccac cacggcgcgc cctgcgggat cggctgcagc gcgcccatgt
     1381 aggtggcgct gaccaccagc agcaccgccc aggccagcat gatcagcgcc gccggcagca
     1441 cgcgatcgag catcaccagc gccacggcga gcagcgcgac gccgaacacc gccttgacct
     1501 tgtccatcca gtgcccggcg cgcggcagga agtgtccggc gctggcgccg atcgcgagca
     1561 gcggcacacc catgccgagg ctcatcgcga acagcgccgc gccgccgagc agccggtcgc
     1621 cggtgctcgc gatcaccgcc agcgcgccga tcagcggcgg cgccacgcag ggaccgacga
     1681 tcagcgccga cagcaggccc atgatcgcga cgccggcgta gtcgccgccg cgctggtggc
     1741 cggaaatctc cgagagccgc gcctgcagcg cgcccggcag ctgcagctgg tacagcccga
     1801 acatcgacag ggcgagcgcc acgaacagca gcgcgaaggc gatcagcacg cccgggtgct
     1861 ggaaggccgc ctgcaggttc tcgccgaagc tcgcggcgac gacgccggcg accgtatagg
     1921 tcgaggccat cgccagcacg tagaccaccg acagtccgaa ggcgtggccg cgcgtcgtgt
     1981 gccggccgcg accgacgatc aggcccgaca ggatcggcac catcgggaac acgcagggcg
     2041 tgaacgacag cagcagcccg aagccgaaga acgccggcag cgccaggaag cgctcgttgc
     2101 gcagcatgcg cgccatgcgg tcctgttcgg gttcgggcgc cggcgccgtg gccgccggcg
     2161 cggcgcccgg gcctgcaggc gccgccgctg ccgtggcgga catcggcgtg gccgttgccg
     2221 ccggcagttc gagcgtccag gtcttcgact gcggcgggta gcagacgccg accggctcgt
     2281 tgcagccctg ccattcggcg cgcagcgtga gcggctgcgc gccgaggccg gcgccgctgc
     2341 gcgtcaggcg cacggtggcg atcgccgctt cgtgccagac ctcggtgcgc ccgaagctcg
     2401 gatcgtcctt gatctcgccg gccggcgtgt cgacgccggc gatcgtgatg ccgggcggca
     2461 gcgcgccttc gggctcgacc ttcagccgcg cgcggtacag gtagtagcca tcggcgatgg
     2521 tccagcgcag gcgcacggcg ccgggctcga ccgccggagc ttcgacgaag aaggcctgct
     2581 cggcgtcgag caggtcgtcg gccgacggct tcttgccgaa caggtcgatg gccatggcgg
     2641 gcggcaggcc ggccacgagc agcatgcagg ccaccggcag ccagcggcgc agcaggacgg
     2701 cgaaacggga catcaggctg gacctccggc gcgggtttcg gcgagcagcc acccgatgta
     2761 cgcaggcgag ccggcttcga tgccgaccgc gatgatttcc ggcaccgtgt acggatgcag
     2821 ttcgcgcagg cgggcggcaa gggcctcgaa ggcgtcggcg cgcgtcttca gcagcatcag
     2881 cacttcggca tcgcgctgca cggccccctg ccagcggtat acggacacga cgcccggcac
     2941 gaggttcgcg caggcggcga ggccttcccc gacgagcacg gtggcgatgt gtccggcgct
     3001 gtcggcgtcg gggcaggtgc agagcacgag ctggcagggc gtatccacgg gatgggcacc
     3061 ttcacggcgg cagagggtcg gcaggcgcag ccggcaagta taaggtgccg ttgcggccat
     3121 gggccgcgta ccggccggct cgtccctcca cgctcacccc tgcacagcgc gcgcatccgg
     3181 ccatcgcgcg caggagcccc ccgtcatgaa gctgcccgcc gtgttgttcg tgctgttcgt
     3241 cgcgctgccg atcgtcgaga tctacgtgct ggtgcgcgtc ggttcggtga tcggtgcgct
     3301 gccgaccatc gcgctggtca tcggcctgtc ggccttcggt gcctggctgg tgcgccatga
     3361 gggcctcggc gtgctgcagc gcgtgcgcac ggccctcgat gccggccagc tgccggcgcg
     3421 cgaactgctc gacggcgcgc tgatcctgat cggcggcgtg ctgctgctga cgccgggctt
     3481 cgtgaccgat gtcttcggtc tctgctgcct gctgccgccg gtgcgcgcgc agctgatccg
     3541 gctgctcgcg cgccatgcca tcgtcgccgc cggccgccgc ggcccgcccg gcggcggcgc
     3601 accgggcgcg cgcggaccgc agaccctgga aggcgagtac cggcgcgagg actgacgccg
     3661 ccggcccggg gacggcggcg tccggggtcc gcagcccatt cccggcgctc ttgaaagccg
     3721 cctgtgcggc cccatttccg gctgcgtccc ttgacagggg acagtcgctg cctaatatca
     3781 gcactcgctc aaggtgagtg ctaacaagtc cggcaccgcc cgtcctccac aacccaaccc
     3841 tcgtatttct gaggagattc gcatgagcct gcgccccctg catgaccgcg tcatcgtcaa
     3901 gcgtgtcgag gaagagacca agtccgccgg tggcatcgtc ctgcccggca acgcagccga
     3961 gaagccgtcg cgcggcaccg tgctcgcgat cggtcgcggc aagattctcg agaacggcga
     4021 cgtgcgcccg ctcgacgtga aggtcggtga ccacgtcctg ttcggcaagt attcgggcag
     4081 cgaagtcaag atcgacggcg acgaagtcct cgtgatgcgc gaggacgaga tcatggccgt
     4141 catcgagaag tgatccggcg cccgccgccg atcgcctgac tgcctgatca cccgttcgtc
     4201 cgttccagaa ttcatttcct gaattccatc cagaattccc gaggtttccg aagcaatggc
     4261 tgccaaagac gtaaagttcg gtgatgacgc ccgttcgcgc atggtgcgcg gcatcaacat
     4321 cctggccaac gcggtcaagg tcaccctcgg tccgaagggc cgcaacgtcg tgctcgacaa
     4381 ggccttcggc gccccgaccg tgaccaagga cggcgtctcc gtcgccaagg aaatcgaact
     4441 gtccgacaag ttcgagaaca tgggcgcgca gatggtcaag gaagtctcct cgaagacttc
     4501 cgatgtcgcc ggcgacggca cgacgaccgc gaccgtgctg gcgcaggcca tcgtccgcga
     4561 aggcatgaag tcggtctcgg ccggcatgaa cccgatggac ctcaagcgcg gcatcgacaa
     4621 ggccgtgcag gccgccgtcg gcgaactcgc caagctgtcc aagccctgcg ccgatgacaa
     4681 ggcgatcgcc caggtcggcg cgatctccgc caacggcgac gagcagatcg gccgcatcat
     4741 cgccgacgcg atggccaagg tcggcaagga aggcgtgatc accgtcgagg acggttcggg
     4801 cctggagaac cagctcgatg tcgtcgaggg catgcagttc gatcgcggct acctgtcgcc
     4861 gtacttcatc aacaaccag
//
LOCUS       DAOQIE010000286         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10080_length_4851_cov_7.023561, whole genome shotgun sequence.
ACCESSION   DAOQIE010000286 DAOQIE010000000
VERSION     DAOQIE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10080_length_4851_cov_7.023561"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="csb2"
                     /locus_tag="PK144_15095"
     CDS             <1..918
                     /gene="csb2"
                     /locus_tag="PK144_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HNF67101.1"
                     /translation="IALRVPGPGRLARLEACHAKGLALGRRIEAPEGRMQPYAPAGID
                     PEPPLPRSRLRFAAVFRRHEGPALPAESIGLLAQTVRATLLSLTPDPVPAVISGHEAD
                     RSPGRRAHLAIVPLPRVEDPHADGTPHGFALLMPDDLAADERAAIGEACLRLDGATLR
                     FRGYGDWHLQRLDAGELTQAARTLQPASWSGPATRWASATPLAFGHHPRPGHPQRDAL
                     VIVARHCADLGLPAPAAVRFGPDSRLIGALPAAAFREGKAGERWCGQFLAHVEIDFAQ
                     PVAGPLVLGAGRHFGLGLMRPLPHPRRCA"
     gene            915..4013
                     /gene="cas3u"
                     /locus_tag="PK144_15100"
     CDS             915..4013
                     /gene="cas3u"
                     /locus_tag="PK144_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HNF67102.1"
                     /translation="MNAVAPLAPLAAADFGAYYAAVHGFTPFPWQQALVDHLAAGRGW
                     PQALDLPTAAGKTSALDAALFHLALEAGLPPAERRAPLRIALVVDRRLVVDGAFTHAG
                     RIAAALGTAETDPAATPVLRTVAARLRVLAGTTAGSPAPVLAVERLRGGVPRENAWAG
                     SPCQPLLLLSTVDQLGSRLLFRGYGLSEFARPLHAGLLGEDSLILLDEAHLSQPFHDT
                     VRAIRRYCGADWRETPPLRPVAIVPLSATQGAAEAGDALRLTPADRAHPELARRLRAA
                     KPAELLACRAEVFIDRLADSARRLLAACPTATATAPHSPGDTADLFAAPAPAAPVVGI
                     VVNRIATARALYAKLRGAGDADALLLIGRMRPLDRDRLVEAYLPRIRAGRAAGDNPRP
                     LYVVATQTIEVGADLDFDALVTESAPLDALRQRFGRLNRLGARSRAPAAILHVSDAGK
                     DDPVYGGAIAATWKWLNAVAVQPAKSKTRGQPAAAPGVDFGIEALAARLREVAPGADC
                     LSPRPQAPVLLPMHVDLLARTSPAPLPSPEPALWLHGPQTTPAEVQLIWRADLPQLHA
                     DETGDLDDDGRVERARELVAALPPSSLEALSLPVWTARAWLADGPLPDEGSDLEGGSV
                     PEDAPLRGGRRALRWGSDGATPVGPRAIRPGDTLIVPAAWGGLDVAAGGAWNPALREA
                     VPDLAEAAARRTHGRTVLRLGPACVAQWRLMLRTGADAEAQAAAVAEAAQQLRKLFAE
                     ADDNPPDARTVLDELRGIAALPVPVCAALDALAAPGRPPRLLRYGGRTQDAELSGWLL
                     AEARPERVAADDGDDASLTVRVGLEAHCRGVGDQASRFAETLGLPPALVDDLGLAGDL
                     HDAGKADPRFQAWLYGGTAPPAAPLLAKSGSLDRLDRQAVASARDLAGYPRGGRHECA
                     SVQLLRAYPGLLATAHDPELVEHLVGSHHGRGRPFLPVIDDPGAATLDASFELFDRRL
                     RLRGPHGLERLDSGWPERFARLQRRYGWWGLAWLEALLRLADHRRSEDERRMRQEETD
                     HE"
     gene            4006..>4851
                     /locus_tag="PK144_15105"
     CDS             4006..>4851
                     /locus_tag="PK144_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67103.1"
                     /translation="MSDTNCQHAAAPCPPLALTGLVGSHPLGALAAFGLLRWSAEIPA
                     LRGARLHWELQDDWIAVLTPPAPLDADALCRLLLDAHVANQWPHLDSDELEMPAEDFR
                     ERCLTHLETSNIQDRFTIDFDAAIGCDAIIGKNGNIKTTLLNMTSGNQSFLDKLKSLK
                     KSLIPINRKKHRQEPSRLICSLKEAMFGPWTYSDDEHPLGWDPTMERDHAFRAQAPTN
                     DDKYRSVRFAIWLAHLSLPVLPCIPSGRHLHTMSFAPERYKPPYRFRWPIWQTPIALD
                     SLSALL"
BASE COUNT          672 a         1808 c         1704 g          667 t
ORIGIN      
        1 atcgcgctgc gcgtgccggg accggggcgg ctggcgcggc tcgaagcctg ccacgcgaaa
       61 gggctggcac tcggccggcg catcgaggcg ccggaaggcc gcatgcagcc gtatgcgccg
      121 gccggcatcg accccgagcc gccgctgccg cgcagccggc tgcgcttcgc cgcggtgttc
      181 cgccgtcacg aaggtccggc gctgccggcc gagtcgatcg gcctgctcgc gcagacggtg
      241 cgcgcgacgc tgctgagcct gacgcccgat ccggtgccgg cggtgatctc gggccacgag
      301 gccgaccgca gtccgggccg gcgcgcgcat ctggcgatcg tgccgctgcc gcgcgtcgag
      361 gatccgcacg ccgacggcac gccgcacggc ttcgcgctgc tgatgccgga cgatctggcg
      421 gccgacgaac gcgccgcgat cggggaagcc tgcctgcggc tggacggcgc gacgctgagg
      481 ttcaggggat acggcgactg gcacctgcag cggctcgatg ccggcgagct gacgcaggcc
      541 gcccgcacgc tgcagccggc gagctggagc ggaccggcga cgcgctgggc ctcggcgacg
      601 ccgctcgcct tcggccacca cccgcgcccc ggccacccgc agcgcgatgc actcgtgatc
      661 gtcgcgcgcc actgcgccga cctcggcctg ccggcaccgg cagcggtccg cttcgggccg
      721 gacagccgcc tgatcggcgc gctgccggcc gcggcattcc gggaaggcaa ggccggcgaa
      781 cgctggtgcg gccagttcct cgcccacgtc gaaatcgact tcgcgcagcc ggtcgccggt
      841 ccgctggtgc tcggggccgg ccggcacttc gggctcgggc tgatgcgccc gctgccgcat
      901 ccgcggaggt gcgcatgaac gccgtcgccc cgctggcgcc gctcgccgcc gccgacttcg
      961 gcgcctatta cgccgccgtg cacggcttca cgccgttccc gtggcagcag gcgctggtcg
     1021 atcacctcgc cgccggccgc ggctggccgc aggcgctcga tctgccgacc gcggctggca
     1081 agaccagcgc gctcgatgcg gcgctgttcc atctggcgct cgaagccggg ctgccgccgg
     1141 cggaacgccg ggcgccgctg cgcatcgcgc tggtcgtcga ccggcggctg gtggtcgatg
     1201 gcgccttcac gcacgccggc aggatcgccg cggccctcgg caccgccgag accgaccccg
     1261 ccgccacccc ggtgctgcgc accgtcgccg cccggctgcg ggtactggcc ggcaccacag
     1321 ccggcagccc ggcgccggtg ctcgcggtcg agcggctgcg cggcggcgtg ccgcgcgaga
     1381 acgcctgggc cggatcgccg tgccagccgc tgctgctgct ctcgaccgtc gatcagctcg
     1441 gctcgcggct gctgttccgc ggctacggcc tcagcgagtt cgcccggccg ctgcacgccg
     1501 gcctgctcgg cgaggacagc ctgatcctgc tcgacgaggc gcatctgtcg cagcccttcc
     1561 acgacaccgt gcgcgccatc cgccgctact gcggggccga ctggcgcgag acgccgccgc
     1621 tgcggccggt cgcgatcgtg ccgctgagcg ccacgcaggg cgcagccgaa gccggcgatg
     1681 cgctgcgcct gacgccggcc gatcgcgcgc atccggaact ggcgcggcgc ctgcgggccg
     1741 ccaagccggc cgaactgctg gcctgcaggg ccgaggtctt catcgaccgg ctcgccgaca
     1801 gcgcccgccg gctgctggcg gcctgtccga cggcgacggc gacggcaccg cactcaccgg
     1861 gcgacaccgc cgacctgttc gccgctccgg caccggccgc gccggtggtc ggcatcgtcg
     1921 tcaaccgcat cgccaccgcg cgggcgctgt acgccaagct gcgcggcgcc ggcgatgccg
     1981 acgcgctgct gctgatcggc cgcatgcgtc cgctcgatcg cgaccggctg gtcgaggcat
     2041 atctgccgcg catccgcgcc gggcgcgctg ccggcgacaa tccgcgcccg ctgtacgtgg
     2101 tcgcgacgca gaccatcgaa gtcggcgccg atctcgactt cgatgcgctc gtcaccgaat
     2161 ccgcgccgct cgatgcgctg cgccagcgtt tcggccggct gaaccggctc ggcgcccgca
     2221 gccgcgcacc ggcggcgatc ctgcacgtca gcgatgccgg caaggacgat ccggtctacg
     2281 gcggcgccat cgctgccacc tggaagtggc tgaacgccgt cgccgtgcag ccggcgaagt
     2341 cgaaaacccg cggccagccg gccgcggcac cgggggtgga tttcggcatc gaggcgctgg
     2401 cggcccggct gcgcgaagtc gcaccgggcg cggactgcct gagcccgcgc ccgcaggcgc
     2461 cggtgctgct gccgatgcac gtcgatctgc tcgcccgcac cagcccggcg ccgctgccgt
     2521 cgccggaacc ggcgctgtgg ctgcacggcc cgcagaccac gccggccgag gtgcaactga
     2581 tctggcgcgc cgacctgccg cagttgcacg ccgacgagac tggcgacctc gatgacgacg
     2641 gccgggtcga gcgggcccgc gagctcgtcg ccgcgctgcc gccgtcctcg ctcgaagcgc
     2701 tgtcgctgcc ggtgtggacc gcgcgcgcct ggctcgccga cggcccgctg ccggacgaag
     2761 gcagcgacct cgaaggcggg tcggtgccgg aagatgcccc gctgcgcggc ggccggcggg
     2821 cgctgcgctg gggcagcgac ggtgccacgc cggtcggccc ccgcgcgatc cgccccggcg
     2881 acacgctgat cgtgccggca gcctggggcg ggctcgatgt cgccgccggc ggcgcctgga
     2941 acccggcgct gcgcgaggcg gtgccggatc tggccgaagc ggcagcgcgc cggacacacg
     3001 gccgcaccgt gctgcgcctc ggtccggcct gcgtcgcgca gtggcggctg atgctgcgga
     3061 ccggggcgga tgccgaggca caggccgccg cggtggccga agccgcgcag cagttgcgca
     3121 agctcttcgc cgaggccgac gacaacccgc cggacgcccg cacggtgctc gatgaactgc
     3181 gcgggatcgc cgccctgccg gtgccggtgt gcgcggcact cgatgcgctg gccgcgccgg
     3241 gcaggccgcc gcggctgctg cgctacggcg gccgcaccca agacgccgag ctgagcggct
     3301 ggctgctggc cgaggcgcgg ccggagcggg tcgcggccga cgatggcgac gacgcctcgc
     3361 tgaccgtgcg cgtcgggctg gaggcgcact gccgcggcgt cggcgatcag gccagccgct
     3421 tcgccgaaac gctcggcctg ccgccggcgc tggtcgatga cctcggtctg gccggtgacc
     3481 tgcacgacgc cggcaaggcc gatccgcgct tccaggcctg gctgtacggc ggcacggcgc
     3541 cgcccgccgc gccgctgctg gccaagtccg gcagcctcga ccggctcgac cgtcaggccg
     3601 tcgccagcgc ccgcgatctg gccggctatc cgcgcggcgg ccggcacgaa tgcgcgtcgg
     3661 tgcaactgct gcgcgcctac cccggcctgc tcgccaccgc ccacgacccg gagctggtcg
     3721 agcatctggt cggcagtcac cacggccgcg gccggccgtt cctgccggtc atcgacgacc
     3781 ccggcgccgc aacgctcgac gccagcttcg agctgttcga ccggcggctg cggctgcgcg
     3841 gaccgcacgg gctggagcgg ctcgacagcg gctggccgga acgcttcgcg cgcctgcagc
     3901 ggcgttacgg ctggtgggga ctcgcctggc tcgaagccct gctgcggctg gccgaccacc
     3961 ggcgctccga ggacgagcgc cggatgcggc aggaggaaac ggatcatgag tgatacgaac
     4021 tgccagcacg cagcagcccc ctgcccgccg ctcgcgctga ccggcctcgt cggcagtcat
     4081 ccgctcggtg cgctggcagc cttcgggctg ctgcgctgga gcgcggagat tccggcgctg
     4141 cgcggggcgc ggctgcactg ggagctgcag gatgactgga tcgcggtgct gacgccgccg
     4201 gcgccgctcg atgccgatgc gctgtgccgg ctgctgctcg atgcgcatgt cgccaatcaa
     4261 tggcctcatt tggacagcga cgaactggaa atgcctgccg aagatttcag agagcgatgc
     4321 ttgacccatt tggaaacatc aaacattcaa gatagattca caatcgattt tgatgccgct
     4381 attggctgcg atgccataat cggcaaaaac ggcaatatca aaacaaccct gctcaacatg
     4441 acatccggca atcagagttt tttggataaa ttaaaatctc taaaaaaatc attgatccct
     4501 ataaatagaa aaaaacatcg ccaagaacct tcgcgtctga tctgctcatt aaaagaagcc
     4561 atgttcgggc catggacata ttctgacgat gagcacccgc ttggctggga tcccactatg
     4621 gagcgggacc atgcattccg cgcacaagcg ccaaccaatg acgacaagta tcgatcagtc
     4681 aggtttgcca tttggttggc acacttgtca cttccggtgc ttccatgcat cccatcaggt
     4741 cgccatttgc acacaatgtc tttcgcacca gaaagataca agccaccgta ccgattcaga
     4801 tggccaatct ggcaaacccc aatagcactg gattcattaa gcgcattgct a
//
LOCUS       DAOQIE010000287         4841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10107_length_4841_cov_4.849770, whole genome shotgun sequence.
ACCESSION   DAOQIE010000287 DAOQIE010000000
VERSION     DAOQIE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10107_length_4841_cov_4.849770"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1267)
                     /locus_tag="PK144_15110"
     CDS             complement(17..1267)
                     /locus_tag="PK144_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNF67104.1"
                     /translation="MPNRCLRRPAAALVLSVAALLAGGCATSPAAPPVTRPPFAGNVD
                     FDAWKRDFAAEARTAGISEVTVSTVVPRLRQLPELLPLDRQQPEFVKPIWSYIDATVS
                     NARIERGRRLRAEHAATLDAIAARSGVPAEIIVAIWGKETDFGGYTGRTPVYDALATL
                     ASTGRRQAFYRSELLAALTLIDRGMLPASPQPTGSWAGAMGQTQFMPGTWLRHAVDGD
                     GDGRIDLYGSLADAFASTAAYLAASGWPAGQPWGWEVRLPAGFDWSLAGEDRAQPLAA
                     WAAAGVTRADGRPLAGGLNATLLVPTGRQGPVFLLTDGFRAIMKYNASTSYALAVALL
                     GDQLAGRPGLAADWPRSQPLLSVAETRELQERLAARGYDSGTPDGMLGTRTRTAVRGY
                     QQRAGMVPDGWPTAELLARLRAEG"
     gene            1390..1812
                     /locus_tag="PK144_15115"
     CDS             1390..1812
                     /locus_tag="PK144_15115"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF67105.1"
                     /translation="MSDVNVSTVVPVTADQIWKMIGGFHALSAWHPAIETSELEDGGR
                     IRRLKLVGGGEIVEKLERFDDSAHTYTYSILEGPLPVKDYTATIKVTPVDGGGSKLEW
                     SSNFEPAGLPENDAVAAIRGVYEAGFDNLKKLLGLAKG"
     gene            2011..2343
                     /locus_tag="PK144_15120"
     CDS             2011..2343
                     /locus_tag="PK144_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67106.1"
                     /translation="MTDALILEARALAGTNPTLIELIRFTRAHDPTDDFRQQQGSQFE
                     ARRDEMLAAAQAFVFTGTPFTGSAAEVLLCMAHCVASAPYLGWSQAQVELYLGTLLRR
                     IAEATPSA"
     gene            complement(2340..4385)
                     /gene="brxL"
                     /locus_tag="PK144_15125"
     CDS             complement(2340..4385)
                     /gene="brxL"
                     /locus_tag="PK144_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNF67107.1"
                     /translation="MQLDDLDRQAAMAFEGYVVRKDLVRTFSRQFPVPTYVVEFLLGR
                     YCATTDEAEIQEGLDIVQRQLGSRTVKAGEEELFKARARESGSVRIIDLITARLDART
                     DSYVASLPSLRLNDVRLTPEMVRDNERMLTGGFYAEVTLGYDASIAQEKGGRPFGVDA
                     LRAIQLSRREVLDVLAAGRGSFTTAAWRDLLLRSVGFEPETLSERARDALLLRMVPFV
                     ERNYNLVELGPRGTGKSHLFQQVSPYAHLISGGKATVARMFVNNATGQRGLVCQYDVV
                     CFDEVSGVSFDQKDGVNIMKGYMESGEFSRGRESIRADGSIVLVGNFEVDVEHQQRIG
                     HLFGPLPPEMRDDTAFMDRIHGFLPGWDVPKLNPALFTGHFGLVSDFLSECFAQLRQQ
                     SRVSAMQGRVYWGSALSGRDLNAVNKTVSGLLKLLYPDAQMPVSDADMEWAVRLALEV
                     RRRVKEQQKRIGAAEFRNTHFSYQLGAEGVEKFVSTPELQSQGGIGDEPLECGQIWTL
                     SPGGAEEHPGLFRLEAAEGPGSGVRILNNPVPAAFKESIRCAEQNLYSRARQLVGDRD
                     PRAHEFSVQLRGFDSARSGAKTGVAALIALSGALLKKSVRGGLIVAGEVTLGGTIEPI
                     HNAVTLAEIAVEKGARSLLLPVSCRRQLFELSDEMATRLDIEFYQDGRDALLKALAD"
     gene            complement(4388..>4841)
                     /locus_tag="PK144_15130"
     CDS             complement(4388..>4841)
                     /locus_tag="PK144_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type B"
                     /protein_id="HNF67108.1"
                     /translation="ARGTGLTFGWDWCRDLQIAYAPGISNFIAGSEYAHGGISLQECL
                     VPVLDLACERAGAADPGGGLTIRSVSWKGLRCTVMVDGASAGQRVDIRTRAGLASSSL
                     AAGTKVLTDGKASLAVADDEQAGAAAVVVVLGADGAVLQKLATTVGDH"
BASE COUNT          834 a         1698 c         1565 g          744 t
ORIGIN      
        1 gggcggcgga cggctgtcag ccttcggcgc gcagccgcgc cagcagttcc gcggtcggcc
       61 agccgtccgg caccatgccg gcccgctgct ggtagccgcg caccgccgtg cgcgtgcgcg
      121 tgccgagcat gccgtcgggc gtgccgctgt cgtagccgcg cgcggccaga cgctcctgca
      181 gctcgcgcgt ctcggccacc gacagcagcg gctgcgagcg cggccagtcg gcggcgagcc
      241 ccgggcggcc ggcgagctgg tcaccgagca gcgccacggc cagcgcgtac gaggtcgagg
      301 cgttgtactt catgatcgcg cggaagccgt cggtcagcag gaacaccggc ccctgccggc
      361 cggtcggcac cagcagcgtc gcgttcaggc cgccggcgag cggcctgccg tcggcgcgcg
      421 tgaccccggc tgccgcccag gccgcgagcg gctgcgcacg gtcctcaccg gccagcgacc
      481 agtcgaagcc ggccggcagg cgcacctccc agccccaggg ctgaccggcc ggccagccgc
      541 tggcggcgag gtaggccgcg gtcgaggcga aggcatcggc gaggctgccg tagaggtcga
      601 tgcgcccgtc gccgtcgcca tcgaccgcgt gacgcagcca ggtgccgggc atgaactgcg
      661 tctgacccat cgccccggcc caggagccgg tcggctgcgg actggccggc agcatgccgc
      721 gatcgatgag cgtcagtgcc gccagcaact cgctgcggta gaacgcctgt cgccggccgg
      781 tcgaggccag cgtcgccagc gcgtcgtaga ccggcgtgcg gccggtgtag ccgccgaagt
      841 cggtttcctt gccccagatg gcgacgatga tctcggccgg cacgccgctg cgtgcggcga
      901 tggcatcgag cgtcgccgcg tgctcggcgc gcagccgccg gccgcgctcg atgcgcgcgt
      961 tcgagacggt cgcatcgatg tacgaccaga tcggcttcac gaactccggc tgctggcgat
     1021 cgagcggcag cagctccggc agctggcgca ggcgcggcac gacggtggaa accgtcactt
     1081 ccgaaatgcc ggcggtgcgc gcctcggcgg cgaagtcacg cttccatgcg tcgaaatcga
     1141 cgttgccggc gaacggcggg cgcgtgaccg gcggcgcggc cggggacgtg gcgcagccgc
     1201 cggcgagcag cgcggcgaca gacaggacga gggcggcggc ggggcggcgc aggcagcggt
     1261 tcggcatgga cggcacggaa gcgggggaac ggacggccag tgtagcgcgc gccgcttgcg
     1321 gccccgcccg ccggcgccta gcttcgacgc gccccccggc atcccccaca cagaggcagg
     1381 aaaatccgca tgagcgacgt caacgtcagc accgtcgtac cggtcacggc cgaccagatc
     1441 tggaagatga tcggcggctt ccacgcgctg tcagcctggc atccggcgat cgaaaccagc
     1501 gaactcgagg acggcggccg catccggcgc ctgaagctgg tcggcggcgg cgagatcgtc
     1561 gagaagctcg aacgcttcga cgacagcgcc cacacctata cgtattcgat cctggaaggc
     1621 ccgctgccgg tcaaggacta taccgccacg atcaaggtca cgccggtcga cggcggcggc
     1681 tcgaagctcg aatggtcgag caacttcgaa ccggccggcc tgcccgagaa cgacgccgtc
     1741 gccgcgatcc gcggcgtcta cgaggccggc ttcgacaacc tgaagaagct gctcggcctc
     1801 gcgaagggct gatgccgatc ataccgatca cacgtaaacg cgcggttttt ctccccctct
     1861 ccccaagccc tctcccgcga ggggcgaggg gcgaggggct gaaaagcgaa gcgttctgat
     1921 gtgatcggta tgagccgccg ctgtcctccg gccgcagtcc cctcgactgc gaccggagac
     1981 tcccgtccgc tgccaccgca gacgccaccg atgaccgatg cactgatcct tgaggcccgc
     2041 gccctcgccg gcacgaaccc gacgctgatc gagctgatcc gcttcacgcg cgcccacgac
     2101 ccgacggatg acttccgcca gcagcagggt tcgcagttcg aagcgcgccg tgacgaaatg
     2161 ctggctgcag cgcaggcgtt cgtgttcacc ggcacgccgt tcacgggcag cgcagcggaa
     2221 gtattgctgt gcatggcgca ttgcgtggcg agtgcgccgt atctcggctg gtcgcaagcg
     2281 caggtcgagt tgtatctggg gacgctgctg cgacggattg cggaggccac accgtccgct
     2341 taatcggcta gcgctttcag cagtgcgtcc cggccatcct gatagaactc gatatccaga
     2401 cgggtagcca tctcatcgga gagttcgaac agctgccggc gacaggaaac cggtaaaagc
     2461 aaggatctgg cgcctttttc gacggcgatt tcggccagtg tcaccgcgtt gtggataggt
     2521 tcgatggttc cgcccagcgt cacttcaccc gcgacgatca gccccccgcg cacggatttc
     2581 ttcagcagcg caccggacag ggcaatgagc gcagccacac cggtcttggc gccggatcgg
     2641 gcagaatcga atccacgcaa ttgcaccgaa aattcgtgcg cgcgcggatc gcggtcaccc
     2701 accagttgcc gggcgcggct gtaaaggttc tgttcggcac aacgaatcga ttccttgaac
     2761 gcagccggca ccggattgtt cagtatccgg acaccgctgc cgggaccttc ggccgcttca
     2821 aggcggaaca gtccgggatg ctcctccgct ccgcccgggc tgagcgtcca gatctggcca
     2881 cactccagcg gctcgtcgcc gatgcctccc tggctctgca gctccggcgt actaacgaac
     2941 ttctcgacac cttcggcgcc cagctgataa ctgaaatggg tgttgcgaaa ctcggccgca
     3001 ccgatgcgct tctgctgctc cttgacccgc cggcgcacct ccagcgccaa acgcacggcc
     3061 cactccatat ccgcatcgct gaccggcatc tgcgcatccg gatacagcag cttgagcagg
     3121 ccgctgaccg tcttgttgac ggcattgaga tcccgccctg ataaagcact gccccagtag
     3181 acgcgcccct gcatggccga gacccggctc tgctggcgta gctgggcaaa acattcggac
     3241 agaaagtcgc tgaccagacc gaaatgcccg gtgaacagtg cggggttgag cttcggcaca
     3301 tcccagcccg gcaggaaacc gtggatgcga tccatgaagg ccgtgtcatc gcgcatctcg
     3361 ggcggcaatg ggccgaacag atgaccgatg cgctgctggt gttcgacatc gacctcgaag
     3421 ttgcccacga gcacgatgga accgtccgcc cggatgcttt ccctgccgcg ggaaaattcg
     3481 ccgctctcca tgtagccctt catgatgttg acgccgtcct tctgatcaaa ggaaacgccc
     3541 gacacctcgt cgaagcagac cacgtcgtac tggcacacca gcccgcgctg gccggtggcg
     3601 ttgttgacga acatccgggc cacggtggcc ttgccacccg aaatgagatg cgcatacggc
     3661 gacacctgct ggaacaggtg cgacttgccg gtgccgcgcg gcccaagctc caccaggttg
     3721 taattgcgct cgacgaacgg aaccatccgc agcagcagcg catccctggc acgttcggac
     3781 aaggtttcgg gctcgaatcc gacactgcgc aacaacaggt cgcgccacgc ggccgtcgtg
     3841 aacgacccgc gacctgccgc caggacatcc agcacctcgc gcctggaaag ctggatggcg
     3901 cgcaaggcgt ccacgccgaa gggacgtccg cctttctcct gcgcaatcga ggcgtcgtag
     3961 ccgagtgtga cctcggcata gaagccaccg gtcagcatgc gctcgttgtc gcggaccatc
     4021 tccggcgtca gccgcacatc gttcaagcgc aggctgggca ggctggcgac ataggaatcg
     4081 gtccttgcgt cgagccgcgc cgtgatgaga tcgatgatcc tgaccgagcc cgattcgcgc
     4141 gcccgggcct tgaacaattc ctcttctccg gccttgaccg tgcgcgagcc gagctggcgc
     4201 tgcacgatgt cgagtccctc ctggatttcg gcttcgtccg tcgtcgcgca gtagcgtccg
     4261 agcaggaact cgacgacgta ggtaggcacc ggaaactgcc ggctgaaggt gcgtaccagg
     4321 tccttgcgta cgacgtagcc ttcgaaggcc atcgcggcct gtcggtccag atcgtcgagt
     4381 tgcatgctca gtgatctcct accgtggtgg ccagcttctg cagcactgca ccgtctgctc
     4441 ccagcaccac caccacagcg gctgcaccgg cctgttcatc atcggccacc gccagactgg
     4501 ccttgccatc cgtcagcacc ttggtgccgg ccgccagcga actggacgcc agccccgccc
     4561 gggtccggat gtccacccgc tgacctgcgc tggctccgtc caccatgacg gtacagcgca
     4621 gacccttcca gctcaccgac cggatcgtga gcccgcctcc cggatcagcg gccccggcgc
     4681 gttcacaggc cagatccaga accggcacca gacactcctg caggctgatg ccaccatgcg
     4741 catattcgga gccggcgatg aagttcgaaa tacccggtgc gtatgcaatc tgcaggtccc
     4801 ggcaccaatc ccatccgaaa gtcagtccgg tgccccgggc a
//
LOCUS       DAOQIE010000288         4770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10335_length_4770_cov_8.061294, whole genome shotgun sequence.
ACCESSION   DAOQIE010000288 DAOQIE010000000
VERSION     DAOQIE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10335_length_4770_cov_8.061294"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1465)
                     /locus_tag="PK144_15135"
     CDS             complement(230..1465)
                     /locus_tag="PK144_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HNF67109.1"
                     /translation="MNAPRPVHAPMTQFPVVDGCLSIGGVPLPVLAARVGRTPFYAYD
                     RVLIAARVAELRAALPAGIELHYAMKANPMPAVVQLLAGLVDGLDVASAGELQVALDT
                     GIDAREISFAGPGKRDAELRAAIAAGITINLESFGETERVARIGDELGLHPSVAVRVN
                     PDFELKAAGMKMGGGAKPFGVDAELVPELLARIGSLDLRFRGFHLFWGSQSLRAETIV
                     DAHRQTFALGLRLADAAPAPVELFNIGGGFGIPYFPGETRLDLAPIADNLETLLPQLA
                     ARQPQARPVIELGRYLVGEAGIYVCEVLDRKVSRGQVYLVTDGGLHHHLAASGNFGQV
                     IRKNYPVAIGTRMDSPATEIASVVGPLCTPLDLLGERMALAAAEPGDLVVVFQSGAYG
                     RSASPAGFLSHPACAEVLV"
     gene            complement(1462..3057)
                     /locus_tag="PK144_15140"
     CDS             complement(1462..3057)
                     /locus_tag="PK144_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HNF67110.1"
                     /translation="MPGLLHELIFARAERTPSAIALRLRDAEQDYATLAEAVGRFAAA
                     CAGRGLEAAGRVAIWLPKRFETVAAIFGACAAGGAFVPVNPLLKPEQVGYILNDCNVR
                     ILVTDRERARLLAGTLAGCADLHTLVVLDSRGPEDLPKLAHVESLAWETLLAEGERRP
                     VHRRIDTDLVSILYTSGSTGRPKGVCLSHRNMLTGAWSVAQYLGNSADDHLLAVLPFS
                     FDYGLSQLTTAFHVGARVTLMDYLLPRDVVRAVAKHGITGLAAVPPLWVQLAQLDWPA
                     EAVASLRYLTNSGGKMPRATLEALRAKLPETTPFLMYGLTEAFRSTYLPPAELDRRPD
                     SIGKAIPYAEILVVREDGSACAPGEPGELVHRGSLVGLGYWNDPVKTAERYKPAPGQP
                     AGLVNPELAVWSGDTVRMDEDGYLYFIGRRDDMIKTSGYRVSPTEVEEVLYGSGLVAE
                     AAAIGVAHGTLGQAVVAVIQPAADAPADDEALLAHCKRVLPNFMVPLCFVRRAALPRN
                     PNGKIDRKQLAGELETLYAETGA"
     gene            complement(3197..4042)
                     /locus_tag="PK144_15145"
     CDS             complement(3197..4042)
                     /locus_tag="PK144_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNF67111.1"
                     /translation="MSRVQPLVAADSTAAESGLDPLLQHPDLNDSSRSTLADTFSKYF
                     YVMRADTPALREEVYRLRYEVYVREFAYEAEEHFADGMERDDYDDHSIHCLLRHRATG
                     LAAGTVRMVCTDPADRNAPLPFERYCLHALRSDQFDPKRVPRHSIGEVSRLAILQHFR
                     RRLTDERKPFSITQEQIKAIDSAEHGRENFPILPVSLFLVSIAMMMYCGVHHGIAMME
                     PRLARLLRRFGLPAKQVGDVIDYHGPRAPFLLDPEEMFAAVNPDLYKLVLALDQQMSR
                     NAHDS"
     gene            4363..4590
                     /locus_tag="PK144_15150"
     CDS             4363..4590
                     /locus_tag="PK144_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNF67112.1"
                     /translation="MVLILYGTSECHLCEQAQEQVLAAAGRPAIEIDVAEDDGLYQRY
                     GLRIPVLRRQDTGAELDWPFDTGAVIALLGA"
BASE COUNT          722 a         1547 c         1806 g          695 t
ORIGIN      
        1 agagcaggcc gcagccgagc gcgagcagcg ccagcggcag cttgagcacg ggatagccgg
       61 cgaggccgag ggcggtgacg gccgccagcg ccagcccgac cagcgccggc agcgggcggg
      121 gcagggtgcg ggcgctgccg gacagcgggg ccgcggcggg caggactcgg gacatcgggc
      181 gggcttccgt taccgcagcg gcttcagcgc attcaagggc cggttcccgt cagaccagca
      241 cttcggcaca ggccggatgc gacaggaagc cggccgggct ggcgctgcgc ccgtaggcgc
      301 cggactggaa cacgacgacc agatcgccgg gttcggccgc tgccagcgcc atgcgctcgc
      361 cgagcaggtc gagcggcgtg cacagcgggc cgacgacgct cgcgatctcg gtggcggggc
      421 tgtccatgcg cgtgccgatc gcgaccgggt agttcttgcg gatcacctgc ccgaagttgc
      481 cgctggcggc gaggtggtgg tgcaggccgc cgtcggtgac cagatacacc tggccgcgcg
      541 agaccttgcg atccagcacc tcgcagacgt agatgccggc ctcgccgacc agatagcggc
      601 cgagctcgat gaccggccgt gcctgcggct ggcgtgccgc gagctgcggc agcagggttt
      661 cgaggttgtc ggcgatcggc gccaggtcga gccgggtctc gccggggaag tagggaatgc
      721 cgaagccgcc gccgatgttg aacagctcca ccggcgccgg tgccgcatcg gccagccgca
      781 ggccgagcgc gaaggtctgg cggtgcgcat cgacgatggt ctcggcgcgc aggctctgcg
      841 agccccagaa caggtgaaag ccgcggaagc gcaggtcgag cgagccgatg cgcgcgagca
      901 gctccggcac cagttcggca tcgacgccga agggcttggc gccgccgccc atcttcatgc
      961 cggcggcctt cagctcgaag tccgggttca cgcgcacggc gaccgacggg tgcaggccga
     1021 gctcgtcgcc gatgcgcgcg acgcgctcgg tctcgccgaa ggattcgagg ttgatcgtga
     1081 tgccggcggc gatcgcggcg cgcagctcgg catcgcgctt gccggggccg gcgaagctga
     1141 tctcgcgggc atcgatgccg gtgtcgagcg cgacctgcag ctcgccggcc gaggccacgt
     1201 cgaggccatc gacgaggccg gcgagcagtt gcacgaccgc cggcatcggg ttggccttca
     1261 tcgcgtagtg cagctcgatg ccggccggca gcgcggcgcg cagctcggcg acgcgcgcgg
     1321 cgatcagcac gcggtcgtag gcgtagaacg gcgtgcggcc gacgcgcgcg gccagcaccg
     1381 gcagcggcac gccgccgatg ctcaggcagc cgtcgacgac cgggaactgc gtcatcggtg
     1441 catgcacggg gcggggagcg ttcatgcgcc ggtctccgcg tacagggttt cgagttcgcc
     1501 ggcgagctgc ttgcggtcga tcttgccgtt cggattgcgc ggcagcgcgg cgcggcgcac
     1561 gaagcagagc ggcaccatga agttcggcag cacgcgcttg cagtgcgcga gcagggcctc
     1621 gtcatcggcc ggggcgtcgg cggccggctg gatcaccgcg accacggcct gaccgagcgt
     1681 gccgtgcgca acgccgatcg ccgcggcttc ggcgaccagg ccggaaccgt acaggacttc
     1741 ctcgacctcg gtcgggctga cccggtagcc ggaggtcttg atcatgtcgt cacgccggcc
     1801 gatgaagtac aggtagccgt cctcgtccat gcgcacggtg tcgccggacc acacggcgag
     1861 ttccggattc accagtccgg ccggctggcc cggcgccggc ttgtagcgct cggcggtctt
     1921 gaccggatcg ttccagtagc cgagcccgac cagcgagccg cggtgcacga gctcgccggg
     1981 ttcgccgggg gcgcaggcgc tgccgtcctc gcgcacgacc aggatctcgg cgtacgggat
     2041 cgccttgccg atcgaatccg gccggcggtc gagttcggcc ggcggcaggt aggtggagcg
     2101 gaaggcttcg gtgaggccgt acatcaggaa gggcgtcgtc tccggcagct tcgcgcgcag
     2161 ggcttcgagc gtcgcgcgcg gcatcttgcc gccggagttg gtcaggtagc gcaggctggc
     2221 gacggcttcc gccggccagt cgagctgcgc gagctgcacc cacagcggcg gcacggcggc
     2281 cagcccggtg atgccgtgct tcgcgaccgc gcgcacgaca tcgcgcggca gcaggtaatc
     2341 catcagcgtc acgcgcgcgc cgacgtggaa ggcggtggtc agctgcgaca ggccgtagtc
     2401 gaagctgaac ggcagcaccg cgagcaggtg gtcatcggcg ctgttgccga gatactgcgc
     2461 gacactccag gcgccggtca gcatgttgcg gtgggaaaga cagacgccct tcggccggcc
     2521 ggtcgaaccg gaggtgtaca ggatcgacac cagatcggtg tcgatgcgcc ggtgcacggg
     2581 gcggcgttcg ccttcggcga gcagcgtctc ccaggcaagc gattcgacgt gcgcgagctt
     2641 cggcaggtcc tcggggccgc ggctgtcgag caccaccagc gtgtgcaggt cggcgcagcc
     2701 ggccagcgtc ccggcgagca ggcgcgcgcg ttcgcggtcg gtgacgagga tgcgcacgtt
     2761 gcagtcgttc aggatgtagc cgacctgctc gggcttcagc agcggattga ccggcacgaa
     2821 ggcgccaccg gccgcgcagg ccccgaagat cgccgcgacg gtctcgaagc gcttcggcag
     2881 ccagatcgcg acgcgcccgg ccgcttcgag gccgcgtccg gcgcaggccg ccgcgaagcg
     2941 tccgaccgcc tcggccagtg tcgcgtagtc ctgttcggca tcgcgcaggc gcagcgcgat
     3001 cgccgaaggc gtccgttcgg cgcgcgcaaa gatcagttcg tgcagtaatc cgggcatgct
     3061 cggcgggctc cgcgggcgga ccgtggggga cggggcaggg ccggcattgt cgcaaacccg
     3121 ggccgctcgg cgggagcggg atcgtagcgt tgcgttgcgt tgggccgcgc ggtggtcgtg
     3181 cgtggggcgc ggcggttcag gagtcgtgcg cgttgcgcga catctgctgg tcgagcgcca
     3241 gcacgagctt gtagagatcg ggattgaccg ccgcgaacat ttcttcgggg tcgagcagga
     3301 agggcgcgcg cgggccgtga tagtcgatca cgtcgccgac ctgcttcgcc ggcaggccga
     3361 agcggcgcag cagccgcgcc agccgcggct ccatcatcgc gatgccgtga tgcacgccgc
     3421 agtacatcat catcgcgatc gacaccagga acaggctgac cggcaggatc gggaagttct
     3481 cgcgcccgtg ctcggcggag tcgatcgcct tgatctgttc ctgggtgatg ctgaagggct
     3541 tgcgctcgtc ggtcaggcgg cgacggaagt gctgcaggat cgcgagccgc gagacttcgc
     3601 cgatgctgtg gcggggcacg cgcttcggat cgaactgatc actgcgcagc gcatgcaggc
     3661 agtagcgttc gaacggcagc ggggcgttgc gatcggccgg gtcggtgcag accatgcgca
     3721 cggtgccggc cgccagaccg gtcgcacggt ggcgaagcag gcaatgtatg gagtgatcgt
     3781 cgtagtcgtc gcgttccatg ccgtcggcga agtgttcctc ggcctcatag gcgaattcgc
     3841 ggacgtagac ctcgtagcgc agccggtaga cttcctcgcg cagcgccggc gtgtcggcgc
     3901 gcatgacgta gaagtatttg gaaaacgtgt cggccagcgt gctgcgcgac gagtcgttca
     3961 gatcgggatg ctgcagcaac gggtcgaggc cggattccgc agcggtggaa tcggcggcaa
     4021 ccagaggctg cacgcggctc acggccgggt ccgtcgcagg ctgggaatga aaggaatcga
     4081 cagccatgat gcggaagtcg ccagcaggga gaaggctggc ggcaggaagc ggacactgtc
     4141 catcccttgc atggttttgg cttcccgtga gctgcgccag aatggcttga acggtacgct
     4201 gcgcgtggtt tccttacgtt ggccggcatg ttggcacagg ccgcgcaccc cgtaaaccgc
     4261 caggagccca tgccgatccg gctccacacg atccggatca cggcaatgtc ggctcggaaa
     4321 tttcacgcac ggccgccggc gatgagcgcg gtcggggagg ccatggtgct gattctgtac
     4381 ggaacttcgg aatgtcatct gtgcgagcag gcgcaggagc aggtgctggc ggcggccggg
     4441 cggccggcca tcgagatcga tgtcgccgag gacgacgggc tgtaccagcg ttatggcctg
     4501 cgcatcccgg tgctgcgccg acaggacacc ggtgccgaac tcgactggcc gttcgacacc
     4561 ggtgccgtca tcgcactgct cggggcatga gcacgatggc acggtcatca gcacgtgtgc
     4621 gtgacgaccg tgcccgccgg gcttcagtcc tcgcgatagc ggcgcggggc gccgtcgtga
     4681 cgacgttcgc cttcgctgcg ccggcggcgg gccgcatcgt cgcgatgcgc gaacgggcgg
     4741 gccgtgtgct cgcgcggagc acgggcggca
//
LOCUS       DAOQIE010000289         4752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10401_length_4752_cov_4.540771, whole genome shotgun sequence.
ACCESSION   DAOQIE010000289 DAOQIE010000000
VERSION     DAOQIE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10401_length_4752_cov_4.540771"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..890
                     /locus_tag="PK144_15155"
     CDS             333..890
                     /locus_tag="PK144_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF67113.1"
                     /translation="MTPRPPPPDVPITQTRLALLQAMPVFGALHAGALGCLLAHAQAV
                     TVARCGWFFHEGETGEQMFVLESGRVAILKAWEGREYLLREFGPGDCFGEIALLDFCP
                     RSASGLALTECSALAFDCSALYALHERDPEQFVLLQMNLGRELCRRLRVADEQLFRTR
                     IAAEAIEGYPLFDGGMHACPIDAGC"
     gene            951..4352
                     /locus_tag="PK144_15160"
     CDS             951..4352
                     /locus_tag="PK144_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNF67114.1"
                     /translation="MCSRCGAPLARACPACGQTVAADARYCSACGGLLPARPAIPAAA
                     TGPAPLEYTPAHLAERILDAQAALAARGGASDGERKTVTALFADLASSTALIHALDPE
                     DARRLLDPVLELMMEAVHHYEGYVTKLLGDGLLALFGAPIAHEDHACRALYAALRMQE
                     AMRRYADGLRAGQGVALALRVGVNTGEVIVRAIRTDDLRTDYDPVGPTIHLASRLEGI
                     ALPGSVIASASTHRLAQGWFTFRALGATPVRGLPEPLPVYELLGPGALRTRLQLMASR
                     GLAAFVGRRAELERLARVWAGVGTAAGGALVALAGEPGVGKSRLLHEFRLGAVPAAQV
                     LETGPVAHGKAWPWLPLIELLTDYAHIDAGDDARTRGGKLSSRVLALDPALDAELPWL
                     LHLLGLDAGLPALAQMDPQSLRQRLFEALARLLRRLARERPLLLIVEDLQWLDADTEA
                     WLAWLAPRLASLPVLLLVDYRPEYRDDWLVAAGALRLRLEALGTTEAGALLDTLLGPA
                     AELAALRAGLLERTDGNPFYLEEVVQTLAGEGVLAGARGNYRLAAAAAAGAARLAHLP
                     PTVQGVLAARIDRLDAADKALLQTLAVIGRHLPWSLIARVTAQPEALLKLRLARLQAG
                     EFIHALPAWPETGYRFRHALTQEVAYGSLPGGRRAALHVQTAQAIEALYPERLDEHCA
                     ELATHYRLGGDAPRAIDWLLRAGLQARRRAASVEAESHLDAALALTLAQPPGPARDRR
                     ELELQLALGSVRMLTQGYAAPGVEQAYLRTLALIETLDEPAGLFPALFGLRTLCHVRG
                     ELVRAQALGARLLELAQRSGDAGLLIEAHRAYGASLFNRGELHAALGHMTRTLALHDP
                     HAHRGHGLVYGVDAGVLGHAFTGWLRWHLGEPDAARREALHALELARADGLAHVEASA
                     LVFAAEVHQHCGDRPRTRALAEAAIALADEHGFPLWSAWGGLLRGWALAAGGETDAGI
                     AAMQAGLIAYARHGAALWRPHFLELLAETCLHAGRLVEARAILDEARTVIATHDERVY
                     ASELWRLDGELTLAEAGPGPLSPVPADAAATCLHTALAHAQACESVAMALRAAVALAR
                     LHVRLGRAATACALLRGALAAFDAAADDAGLTDARRLLARLAQPG"
     gene            complement(4520..>4752)
                     /locus_tag="PK144_15165"
     CDS             complement(4520..>4752)
                     /locus_tag="PK144_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67115.1"
                     /translation="DPLPFAPARLTQGTLVADIVIQSGGTPLLREAARRGLPTHRGIY
                     MLSEEIRSYAEFFGFSWALDRIGWSEQEYESP"
BASE COUNT          602 a         1780 c         1706 g          664 t
ORIGIN      
        1 gacggcggtg cgccaggcgg cggcgcagct gaagcattat ctgggctgag gccggacgca
       61 gtcgggaccg gctgcagccg cgctgccacc ggctcgccgg cagcgggcgg gcgtggctcg
      121 ccacgagtcg cgcccgtctg ctgccggatc ggtgcactcg cggcacatgg tcaccggcac
      181 gccgctcgca tcgcacagcc ggtggttccg gcgtcgccgc tgccggcggt tgcgggcgaa
      241 taccggacgc atgtgccggt aaacgcctga acagcggcct acactggcgc cagggccggc
      301 cgcggacggc ccggtacccg gagcccacgc tgatgacccc acggccgcca ccgccggatg
      361 tgccgatcac gcagacccgg ctggcgctgc tgcaggcgat gccggtgttc ggcgcactgc
      421 acgccggggc gctcggctgc ctgctcgcgc atgcgcaagc ggtgacggtc gcgcgctgcg
      481 gctggttctt ccacgagggc gagaccggcg agcagatgtt cgtgctcgaa tccgggcgcg
      541 tcgcgatcct caaggcctgg gaagggcgcg agtacctgct gcgcgagttc ggccccggtg
      601 actgcttcgg cgagatcgcg ctgctcgact tctgcccgcg cagcgcctcg gggctcgccc
      661 tcaccgagtg ctcggcgctg gctttcgact gcagcgcgct gtatgcgctg cacgagcgtg
      721 accccgagca gttcgtgctg ctgcagatga acctcggccg cgagctatgc cgacgcctgc
      781 gcgtggccga cgagcagctg ttccgcacgc gcatcgccgc cgaggcgatc gagggctatc
      841 cgctgttcga cggcggcatg cacgcctgcc cgatcgacgc cggctgctga ggaccgcgcc
      901 ccgtgcactg cgccgcctgc ggctgcgcac atcccgacgc caccactagc gtgtgcagcc
      961 gctgcggtgc gccgctcgcg cgcgcctgtc cggcctgcgg ccagacggtg gcagcggatg
     1021 cgcgctactg cagtgcgtgc ggcggactgc tgccggcccg accggcgatc ccggcggcgg
     1081 cgaccgggcc ggcaccgctc gagtacacgc ccgcgcacct cgccgagcgc atcctcgatg
     1141 cgcaggcggc gcttgccgcc cgcggcggcg ccagcgacgg cgagcgcaag accgtcaccg
     1201 cgctgttcgc ggatctggcc agctcgaccg ccctgatcca tgcgctcgat cccgaggacg
     1261 cacgccgact gctcgatccg gtgctcgaac tgatgatgga agccgtgcac cactacgagg
     1321 gctacgtcac gaagctgctc ggcgacggtc tgctcgcgct gttcggcgcg ccgatcgcgc
     1381 acgaggacca cgcgtgccgc gcgctgtacg cggcgctgcg catgcaggaa gccatgcgtc
     1441 gctacgccga tgggctgcgc gccgggcagg gcgtcgcact ggcattgcgc gtcggcgtca
     1501 acaccggcga ggtcatcgtg cgtgcgatcc gcaccgatga cctgcgcacc gactacgacc
     1561 cggtcgggcc gacgatccat ctcgcctcgc gcctggaggg catcgcctta cccggctccg
     1621 tgattgcgag cgcaagcacc caccggctcg cgcagggctg gttcacgttc cgtgcgctcg
     1681 gcgccacgcc ggtgcgcggc ctgcccgaac cgctgccggt gtacgagctg ctcgggccgg
     1741 gcgcgttgcg tacgcgcctg cagctgatgg caagccgcgg gctcgcagcg ttcgtcggcc
     1801 ggcgtgccga actcgagcgg ctcgcgcgcg tctgggccgg tgtcggcacg gcggcgggcg
     1861 gtgcgctcgt cgcgctcgcc ggcgagcccg gggtcggcaa gtcacgactg ctccacgagt
     1921 tccggctcgg cgccgtgccg gctgcacagg tgctggaaac cggcccggtc gcgcatggca
     1981 aggcctggcc gtggctgccg ttgatcgaac tgctgaccga ttacgcgcac atcgatgccg
     2041 gcgacgatgc ccgcacgcgc ggcggcaagc tcagcagccg cgtgctggca ctcgaccctg
     2101 cgctcgacgc cgagctgccg tggctgctgc atctgctcgg gctcgacgcc ggcctgccgg
     2161 cgctcgcgca gatggatccg cagagtctgc gccagcgcct gttcgaggcg ctcgcgcgcc
     2221 tgctgcgccg gctggcccgg gaacggccgc tgctgctgat cgtcgaggat ctgcagtggc
     2281 tggatgccga caccgaagcc tggctggcgt ggctcgcgcc gcgcctcgcg tcgctgccgg
     2341 tgctgctgct ggtcgactac cggcccgaat accgcgacga ctggctcgtg gccgccgggg
     2401 cactgcggct gcggctcgaa gcgctcggaa cgaccgaagc cggggcgctg ctcgacacgc
     2461 tgctcggccc ggcggcggaa ctcgcagcgc tgcgcgccgg cctgctggag cgcaccgacg
     2521 gcaatccttt ttacctcgaa gaggtcgtgc agacgctcgc cggcgagggc gtgctcgccg
     2581 gcgcacgcgg caactatcgg ctcgccgccg ccgccgccgc cggcgcggcg cggctcgcgc
     2641 atctgccgcc gaccgtgcag ggcgtgctcg ccgcccgcat cgaccggctc gacgccgccg
     2701 acaaggcctt gctgcagacg ctggcggtga tcggccggca cttgccgtgg agcctgatcg
     2761 cgcgcgtcac cgcgcagccc gaggcgctac tgaagctgcg gctcgcgcgc ctgcaggccg
     2821 gcgagttcat ccacgcactg ccggcctggc ccgagaccgg ctaccgcttc aggcacgcgc
     2881 tgacccagga agtcgcctac ggctcgctgc ccggcgggcg ccgcgccgcg ctgcacgtgc
     2941 agaccgcgca ggcgatcgag gcgctgtacc ccgagcggct cgacgagcac tgcgccgagc
     3001 tcgccacaca ctaccggctc ggcggcgacg cgccgcgcgc gatcgactgg ctgctgcgtg
     3061 ccggtctcca ggcgcgccgg cgcgcggcca gtgtcgaggc cgagtcgcac ctggatgccg
     3121 cgctggcact gacgctcgcg cagccgccgg gcccggcgcg tgatcgccgc gaactggagc
     3181 tgcagctcgc actcggttcg gtgcgcatgc tcacgcaggg ctacgcggcc cccggcgtcg
     3241 aacaggcgta cctgcgcacc ctcgccctga tcgagacgct cgacgagccg gccgggctgt
     3301 ttccggcgct gttcggactg cgcacgctct gccatgtgcg cggcgaactc gtgcgcgcgc
     3361 aggcgctcgg tgcgcgcctg ctcgaactgg cgcagcgctc gggcgacgcc gggctgttga
     3421 tcgaggcgca tcgcgcctac ggagcctcgc tgttcaaccg cggggagctg cacgccgcgc
     3481 tcgggcacat gacgcgcacg ctcgcgctgc acgatccgca tgcgcaccgc ggtcacggcc
     3541 tggtctacgg cgtcgatgcc ggcgtgctcg gccacgcctt caccggctgg ctgcgctggc
     3601 atctcggcga accggacgcc gcgcggcgcg aggcgctgca cgcgctcgag ctcgcgcgcg
     3661 ccgacgggct cgcgcacgtc gaggcctcgg ccctggtgtt cgccgccgag gtacaccagc
     3721 actgcggcga ccgcccccgc acgcgcgcac tcgccgaggc agccatcgcg ctcgccgacg
     3781 agcacggctt tccgctgtgg tcggcctggg gcgggctgct gcgcggctgg gcgctcgccg
     3841 ccggcggcga gaccgacgcc ggcatcgcgg cgatgcaggc cgggctgatt gcctacgcgc
     3901 gccacggcgc cgcgctctgg cgcccgcatt ttctcgaact gctggccgaa acctgcctgc
     3961 acgccggccg gctggtcgaa gcgcgtgcga tcctcgacga ggcgcgcacg gtgatcgcca
     4021 cgcatgacga gcgcgtctac gcatccgagc tctggcggct cgacggcgaa ctcaccctcg
     4081 cggaagccgg ccccggtccg ctgtcaccgg tgccggccga tgcagccgca acctgtctgc
     4141 acacggcact cgcccatgca caggcctgcg aatccgtggc catggcactg cgcgcggccg
     4201 ttgcgctcgc gcgtctgcac gtccggctcg gccgtgcagc cacggcgtgc gccctgcttc
     4261 gcggagcgct cgcagccttc gacgccgctg ccgacgatgc cgggctcacg gatgcgcgac
     4321 ggctgctcgc aaggctcgcg cagcccggct gaaaccgctc accaccgctc atcccgatca
     4381 catcggaacg cagcactttt cagaagcccc ccgccccgcg cgggagaggg ggaagaaaat
     4441 catgcaatca cgggtgatcg gtatcagacg gccttgcggc gcgggcgcat cgtgagcttg
     4501 cgccggatcg gcatgcagct cacggcgatt cgtattcctg ctcgctccag ccgatgcggt
     4561 ccagcgccca cgagaagccg aagaactcgg cgtaggagcg gatttcctcg ctcagcatgt
     4621 agatgccgcg gtgcgtcggc agaccgcggc gggcggcctc gcgcagcagc ggcgtgccgc
     4681 cggactggat gacgatgtcc gcgaccagcg tgccctgggt gagccgcgcc ggtgcgaacg
     4741 gcagcggatc gc
//
LOCUS       DAOQIE010000290         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10411_length_4749_cov_5.203451, whole genome shotgun sequence.
ACCESSION   DAOQIE010000290 DAOQIE010000000
VERSION     DAOQIE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10411_length_4749_cov_5.203451"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="cas7u"
                     /locus_tag="PK144_15170"
     CDS             <1..1020
                     /gene="cas7u"
                     /locus_tag="PK144_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HNF67116.1"
                     /translation="SLDFTPLARTPRLLLQAGLRPLQGSRFQPTGFPDLGPARYDLPN
                     GTPMLLVESSQSIANRLEKVCWNEIENDWIEPLKGLPLVQVTDKDGNFLTNSLLEAHR
                     LNSEYIARTSDFSRIAAEIGFSPDRAFDVRRQLAPVLLKYDINSLLHGVFLEEIAGVI
                     RLPRALSGFIEASNIRTADSGGVKINRQEPTLKEGEGNVIYGRTDFVAETITAFFNLD
                     LAQIRGYGLGAAAEQLLIAVALLKIRLLLDEGLRLRTACDLECVELRVTRPAGWTLPE
                     RAELEAALPGLIEAVAAAGGFAEPRVTPVRWEKGKSAGKPKKGKEAKKPEDGDDDSQT
                     GLDLG"
     gene            1025..2551
                     /gene="csb2"
                     /locus_tag="PK144_15175"
     CDS             1025..2551
                     /gene="csb2"
                     /locus_tag="PK144_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HNF67117.1"
                     /translation="MLAIAFRFPGGRYHATPWGRHVNEAAVAWPPEPWRLLRALLAVW
                     HRKLDPVRWPRAQLAALLDRLAAQPPQFHLPPSMPAHSRHYLPTRDKTTLVFDAFLRI
                     EAGQPVHAVWPTLELDAEQLELLDALLDGLGYLGRAESWVEAVREAPPAGRRPDCVPV
                     EADADAENDGGGGDGGEMLRLLAARPAAGYAGFRSRALTEFGRARRIADTLPADWLDA
                     LAVETGALQKAGWNRPPAAIEIPYRRRPARPSAPRHDRRPGPAIDTVRYALYGRPLPR
                     IEDAIRVGEWLRSTVLRACHPPVPALISGHDLPPGNPHAHAFWLAESAGGDGRIDHVL
                     IHVPDGIPAGVFGVLADPGELREPARRRSAGDGDADDTDTRAWQLLPEWFGRAATPPD
                     GDAGRLLGKATLWRSTTPYLHPWHVKRGFGAAEQIRRECRERGLAEPEVRELEHIEVA
                     GRRLRPLDFHRFRRKPVAIQPDARGHALELRFPEPVSGPLALGFGCHFGLGVFGRGEG
                     "
     gene            2661..2843
                     /locus_tag="PK144_15180"
     CDS             2661..2843
                     /locus_tag="PK144_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNF67118.1"
                     /translation="MKYSEFVRWLKSHGAEISQGKGRHYLKATYNGRTVPVPYHGAKE
                     IPEGVRKSILKQLGLS"
     gene            2892..3317
                     /locus_tag="PK144_15185"
     CDS             2892..3317
                     /locus_tag="PK144_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNF67119.1"
                     /translation="MLAYALNVVPDTVGFMSTCRDLPEFVGVGDSIEDALRLAVEGLE
                     TTIGIYIDERRPVPMPSAPEPGEHAVYVPALTAAKALLSNAMIETGWRKADLVRALGV
                     HPPQVDRLLDVNHHSKLDQIEAALLALGRRLVVEVQRAA"
     assembly_gap    3334..3433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3527..4747
                     /gene="cas7u"
                     /locus_tag="PK144_15190"
     CDS             3527..4747
                     /gene="cas7u"
                     /locus_tag="PK144_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HNF67120.1"
                     /translation="MSTDFILTLDRLQAAVAGAAAIRLRQALEPAGGPGSKVFPPTHE
                     GGQYAWETRRVDGQDVRCVLLDSVQSQANRCELALLDALRDGRLRLPLIEARFAEFSD
                     LGAVSTLEAPHRIADAIFRDSTLDGVPFRDSVIGQAFIESTLRDANGLFRWCPTALVF
                     GMWDSTGSVGGLGTKFARALSAEIIGYGAQAGVHTSSRIDPLGIKNIEIYVTPDEDWT
                     TDAGAAKQGKSGPEKTKPSALNHSNIPPTLDLAEENLATVKKGEPIRGGVTLDRAELC
                     AVLSLPGLRKLRFPQADGTPDAARNHAARTVLAALALAALALQREQGGLDLRSRCLLI
                     PAGLAAYELIAADGSVTPFTLDAEAACALFEAARLAAEAAGLEWPSQPVVLEPQPKLI
                     ELVRRSRAATGVED"
BASE COUNT          739 a         1681 c         1526 g          703 t
ORIGIN      
        1 tccctcgact tcacccccct cgcccgtacc ccgcgcctgc tgttgcaggc cggactgcgc
       61 ccgctgcagg gcagccgctt ccagccgacc ggttttccgg acttgggtcc ggctcgttat
      121 gatctgccga atggcacacc catgctgctc gtcgaatctt cacagagcat tgccaatcgt
      181 ctggaaaaag tttgctggaa cgaaatcgag aacgactgga ttgaaccgct gaaaggctta
      241 ccgcttgttc aggtgaccga caaggacggc aattttttga ccaattccct tctggaggct
      301 caccggctga attccgaata cattgcccgc acttctgatt tctccaggat cgcagccgaa
      361 atcggttttt cccctgatcg cgcattcgac gtccgccgtc aactggctcc cgtcttgctg
      421 aagtacgaca tcaacagcct gctgcatggg gtgtttctcg aagaaatcgc aggtgtcatc
      481 agattgccgc gtgccctgtc cggcttcatc gaagccagca acatccgcac tgccgacagt
      541 ggtggcgtca agattaatcg ccaggaaccc acgctgaagg aaggcgaagg caacgtcatt
      601 tacggccgta cggattttgt cgccgagaca atcaccgcct tcttcaacct cgacctcgcg
      661 caaatccgcg gctacggcct cggggctgcc gccgaacaac tgctgatcgc cgtggcactg
      721 ctgaagatcc ggctgctgct cgacgaaggt ctgcgcctgc gcaccgcctg cgacctcgaa
      781 tgcgtggagc tgcgcgtcac gcgcccggcc ggctggacgc tgccggagcg tgccgaactc
      841 gaagccgcgc tgccggggct gatcgaagcg gttgccgccg ccggcggctt cgccgagccg
      901 cgcgtgacgc cggtgcgctg ggaaaagggc aagtccgccg gcaagccgaa gaagggcaag
      961 gaggcgaaga agccggagga cggcgacgac gacagtcaga ccggcctcga cctgggctga
     1021 cgcgatgctc gcgatcgcct tccgctttcc cggcgggcgc taccacgcca cgccctgggg
     1081 ccgccacgtc aacgaggccg ccgtcgcctg gccgcccgaa ccgtggcggc tgctgcgcgc
     1141 gctgctcgcc gtctggcacc gcaagctcga tccggtgcgc tggccgcgcg cgcagctcgc
     1201 ggcgctgctc gaccggctcg ccgcgcagcc cccgcaattc cacctgccac cgtcgatgcc
     1261 ggcgcacagc cgccattacc tgccgacccg cgacaagacc acgctggtat tcgacgcctt
     1321 cctgcgcatc gaagccgggc agccggtcca tgccgtctgg ccgacgctcg aactcgacgc
     1381 cgagcaactc gaactgctcg atgcgctgct cgacggtctc ggctatctcg ggcgcgccga
     1441 aagctgggtc gaggccgtgc gcgaagcacc gccggccgga cggcggcccg actgcgtgcc
     1501 ggtcgaggcc gatgccgatg ccgaaaacga cggcggcggc ggcgacggcg gcgaaatgct
     1561 gcggctgctc gccgcccgtc cggcggccgg ctacgccggg ttccgcagcc gcgcgctgac
     1621 cgagttcggc cgcgcccggc gcatcgccga cacgctgccg gccgactggc tcgatgcgct
     1681 ggccgtcgag accggcgcgc tgcagaaagc cggctggaac cgcccgcccg cagccatcga
     1741 gattccgtat cgccgccgcc cggcacgacc gtccgcaccc cggcacgacc gccgccccgg
     1801 gccggcgatc gacacggtgc gctacgcgct gtacggccgg ccgctgccgc gcatcgagga
     1861 tgcgatccgc gtcggcgaat ggctgcgcag caccgtcctg cgcgcctgcc acccgccggt
     1921 gcccgcgctg atcagcggcc acgacctgcc gcccggcaat ccccacgccc atgccttctg
     1981 gctcgccgag agcgccggcg gcgacggccg catcgaccat gtgctgatcc acgtgccgga
     2041 cggcatcccg gccggggtgt tcggcgtgct ggccgatccg ggcgaactgc gcgaaccggc
     2101 ccggcgcagg tccgccggag acggcgatgc cgacgacacc gacacccgcg cctggcaact
     2161 gctgcccgaa tggttcggcc gcgccgccac gccaccggac ggggacgccg ggcgactgct
     2221 cggcaaggcc acgctctggc gcagcacgac gccgtacctg cacccctggc atgtgaaacg
     2281 cggcttcggg gccgccgagc agatacggcg cgaatgccgc gagcgcgggc tggcggaacc
     2341 ggaagtccgg gagctggagc acatcgaagt cgccggccgc cggctgcgcc cgctcgattt
     2401 ccaccgcttc cgccgcaagc cggtcgccat ccagcccgat gcccgcggtc acgcgctcga
     2461 actgcgcttt ccggaaccgg tcagcggccc gctggcgctc ggcttcggct gccatttcgg
     2521 actcggggtg ttcggacgcg gcgagggctg aaaccgctca cccggggaca gacatctgac
     2581 ccgcccgtca tcctccggcg cctgtttact cgacatcact tctgttgtat ttcgccttta
     2641 gcaaaccaag aggccggagg atgaagtaca gcgagttcgt cagatggctg aagagtcacg
     2701 gcgccgagat cagtcagggc aagggtcgtc actacctgaa ggcgacgtac aacggcagga
     2761 cggtgccggt gccataccac ggggcgaagg aaatccctga aggcgtccgg aaatcgattc
     2821 tgaaacagct ggggctcagt tgatgcccgg tttctcgctg gctttgcccc cggccgcaac
     2881 aggagaccac catgctggcc tatgccctga acgtcgtccc cgacaccgtc gggttcatga
     2941 gcacctgccg tgacctgccg gaattcgtcg gggtcggcga cagcatcgag gatgccctcc
     3001 ggcttgccgt cgaggggctg gaaaccacga tcggcatcta catcgacgaa cgccgcccgg
     3061 tgccgatgcc ttctgcgccc gagcccggcg aacacgcggt ctacgtgccg gcgctgacgg
     3121 ccgcgaaggc gctgctcagc aacgcgatga tcgaaaccgg ctggcgcaag gccgatctcg
     3181 tgcgggcact cggtgtgcac ccgccgcagg tcgatcgtct gctcgacgtg aaccaccact
     3241 caaagctcga ccagatcgaa gcggccctgc tcgcactcgg ccgtcggctg gtcgtcgagg
     3301 tgcagcgggc agcctgagcg cagcccgccg gcannnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnccggcta gctagtcgct gcccccgcgc cccgccccgc cctgcacgct
     3481 ttgcccatcc gcacatccgc acatccggac accggaggtt tctgtcatga gcaccgactt
     3541 catcctcacc ctcgaccgcc tgcaggccgc cgtggccggc gcagcggcga tccggttgcg
     3601 gcaggcgctg gagccggcgg gcgggccggg cagcaaggtg tttccgccga cgcacgaggg
     3661 cgggcagtac gcgtgggaaa cgcggcgggt cgatgggcag gacgtgcgct gcgtgctgct
     3721 cgattcggtg cagtcgcagg ccaaccgctg cgagctggcg ctgctcgatg cgctgcgcga
     3781 cgggcggctg cggctgccgc tgatcgaggc gcgcttcgcg gagttcagcg acctcggcgc
     3841 ggtcagcacg ctcgaagcgc cgcaccggat cgccgatgcg attttccgcg actcgacgct
     3901 cgacggcgtg ccgtttcgcg acagcgtgat cggccaggct ttcatcgaat cgacgcttcg
     3961 cgatgccaac ggcctgttcc gctggtgccc gacggcgctg gtgttcggga tgtgggattc
     4021 gaccggcagc gtcggcggcc tcggcaccaa gttcgcccgc gcactcagcg ccgagatcat
     4081 cggctatggc gcacaggccg gcgtgcatac ctcgtcgcgc atcgatccgc tcggcatcaa
     4141 gaacatcgag atctacgtca cgcccgatga ggactggacc accgatgccg gtgccgcgaa
     4201 acagggcaag tccggaccgg agaaaaccaa gccctccgcg ctcaaccact cgaacatccc
     4261 gccgacgctc gatctcgccg aggaaaacct cgccaccgtc aagaaaggcg agccgatccg
     4321 cggcggcgtc accctcgatc gcgccgaact ctgcgccgtg ctgtcgctgc cgggcctgcg
     4381 caagctgcgt ttcccgcagg ccgacggcac gcccgatgcc gcgcgcaacc acgccgcccg
     4441 caccgtgctc gcggcgctgg cgctggcggc gctggcgctg cagcgcgagc agggcggtct
     4501 cgatcttcgt tcgcgctgcc tgctgatccc ggccggtctg gctgcctacg agctgatcgc
     4561 cgccgacggc agcgtcacgc cgttcacgct cgatgccgag gccgcctgcg cgctgttcga
     4621 ggctgcgcgc ctggcggccg aagcggccgg gctggagtgg ccgtcgcagc cggtcgtgct
     4681 cgaaccgcag ccgaagctca tcgaactggt gcgccgcagc cgcgcggcga ccggggtcga
     4741 ggactgaaa
//
LOCUS       DAOQIE010000291         4737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10451_length_4737_cov_7.187313, whole genome shotgun sequence.
ACCESSION   DAOQIE010000291 DAOQIE010000000
VERSION     DAOQIE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10451_length_4737_cov_7.187313"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(756..3107)
                     /locus_tag="PK144_15195"
     CDS             complement(756..3107)
                     /locus_tag="PK144_15195"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNF67121.1"
                     /translation="MSEDVKSDELKAMALHYHRFPVPGKIEVVPSKPLANQRDLALAY
                     SPGVAAACEAIVEDPQEAATVTARGNLVAVISNGTAVLGLGPIGALASKPVMEGKGVL
                     FKKFAGINVFDIEVDERDPDKLIDIIASLEPTFGGINLEDIKAPECFYIEQKLRERMK
                     IPVFHDDQHGTAIITTAAVLNGLRVVGKDITKVRLTCCGAGAAGLACLDLLVSLGLKK
                     ENIIVCDSKGVVYKGRTVYMDDRKAGYAADVPHRTLGEAVVGADIFLGVSGPGVLTQD
                     MVKTLAPNPLILALANPTPEILPELAKAVRPDAILATGRSDYPNQVNNVLCFPYIFRG
                     ALDVGASTINEEMKLACVRAIADLTMQEPDEAVMAAYGDQPLHFGPDYLIPKPFDPRL
                     ILELPVAVAKAAMETGVATRPITDFDAYREQLSQYVYRSGMIMKPVFERAKADPKRIV
                     YCEGEDDRVLRAVQVVCDDELGRPMLIGRRERIAERLEQLSLHVKMDEDFDVIDFRDH
                     PHYEACWQEYHRLLARRGMTIENARQRVRTRPTVLGALLVRLGLADTVICGMQTRYRR
                     QLEHMIDVFGIQSGVPTPAAMSLLLTPHGPLFLCDTHVNFDPTAEQIASMTIMAADEV
                     RRFGLTPKVALVSHSNFGSDYTPTSRKMAEALQLLLKRAPDLEVEGEMQGDTALSDAL
                     RSRLFPESRLSGTANLLVLPNLDAANIAYNLLKMVADSIAIGPILMGLNLPGHVMTTA
                     ATVRRIVNMSAVAVVDAQVYAASRAGHAPAAPSAAAGGLPQGA"
     gene            3608..>4737
                     /locus_tag="PK144_15200"
     CDS             3608..>4737
                     /locus_tag="PK144_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNF67122.1"
                     /translation="MSTIRQEDFIQSIADAFQYISYYHPVDYIQALSAAYEREQNPAA
                     KDAIAQILINSRMCAEGHRPICQDTGIALVFLKVGMHVKWDAKMSVTAMVNEGVRRAY
                     LHPDNKLRASVLTDPAGKRTNSKDNTPAVIHYEIVDGDGVEVICAAKGGGSEAKSKFA
                     MLNPSDNLVEWVLKTVPTMGAGWCPPGILGIGIGGTPEKALLLAKESLMAPVDIQDLI
                     ARGPSNRAEELRLELYEKVNALGIGAQGLGGLTTVLDVKVLDYPTHAANLPIAMVPNC
                     AATRHVHFHLHGEGPAVLTPPSLDDWPKLTYDSSKGKRVNLDTVTRAEVAGWKPGDVL
                     LLNGKLLTGRDAAHKRMTDMLNRGEKLPVDFNGRFIYYVGPV"
BASE COUNT          814 a         1586 c         1564 g          773 t
ORIGIN      
        1 tctcgctgtc gcagatcggc cagcagctcg gcctcgaaat ccacccgcag gcgcgaccga
       61 cgaccttcgc cgagatcgag agcgcgctga tgcgcggctg aggcgccgcg atcttccccg
      121 ctgttcagcc gatacaaacg acaagggcgc cgcgaggcgc ccttgctgtt ttccggctcc
      181 tgcggctgcg aagcacgccg tttcgggcgt atcgaccgca agcgcacggc gcccgcgcga
      241 accgcgctgc catcgacgcg aacggcacac ccgagcgtgt gacccacggt ttaatccgat
      301 tgaacagcac gcctgagcgt gtgacccgcg gtttaatctg attaaacagc acgcccgagc
      361 gtatgacccg cggtttaatc tgattaaaca gcactcccga gcgtgtgaac cgcggtttaa
      421 tctgattaaa cagcacacct gagcgtgtga cccacggttt aatctgatta aacagcccac
      481 ctgagcgtgt gacccgcgct tccatcgacc gcaaccgcag tctcgtcgac cggcaccgcg
      541 gttgtgcgca ccgggtgccg gtcacggcag aacccgtcac cggttccgcc cccccacccg
      601 cacgggcggc gaggggctga taccgatcac acgtaaacgc atggtttttc tccccctctc
      661 cccaacccct ctcccgcgag gggcgagggg ctgaaaagcg aagcgttctg atgtgatcgg
      721 tatgaaaccg ctgaacggcg cgggtcagcg cggtatcacg ccccctgcgg cagtccgccc
      781 gccgccgccg acggtgcggc cggggcgtgg ccggcacggc tggcggcgta gacctgcgca
      841 tcgaccaccg ccaccgcgct catgttgacg atgcggcgca cggtcgctgc cgtcgtcatc
      901 acgtgacccg gcaggttcag gcccatcagg atcgggccga tcgcgatcga gtcggcgacc
      961 atcttcagca ggttgtaggc gatgttggcg gcgtcgaggt tcggcagcac cagcaggttg
     1021 gcggtgccgc tcaggcgcga ctccgggaac aggcggctgc gcagcgcatc ggacagcgcc
     1081 gtgtcgccct gcatctcgcc ttcgacttcg aggtccgggg cgcgcttgag cagcagctgc
     1141 agcgcctcgg ccatcttgcg cgaggtcggc gtgtagtcgc tgccgaagtt cgagtgcgag
     1201 accagcgcca ccttcggcgt caggccgaag cggcgcacct cgtcggcggc catgatcgtc
     1261 atcgacgcga tctgctcggc ggtcggatcg aagttgacgt gcgtgtcgca gaggaacagc
     1321 gggccgtgcg gcgtcagcag caggctcatc gccgccggcg tcggcacgcc ggactggatg
     1381 ccgaacacgt cgatcatgtg ttcgagctgg cggcggtagc gcgtctgcat gccgcagatc
     1441 acggtgtcgg cgaggccgag gcgcaccagc agcgcaccga gcacggtcgg gcgcgtgcgc
     1501 acgcgctggc gggcgttctc gatggtcatg ccgcggcgcg cgagcaggcg atggtattcc
     1561 tgccagcagg cttcgtagtg cggatggtcg cggaagtcga tgacatcgaa gtcctcgtcc
     1621 atcttcacgt gcaggctgag ctgttcgagg cgttcggcga tgcgctcgcg ccggccgatc
     1681 agcatcgggc ggccgagttc gtcgtcgcag accacctgca cggcgcgcag cacgcgatcg
     1741 tcctcgccct cgcagtagac gatgcgcttc ggatcggcct tcgcgcgctc gaacaccggc
     1801 ttcatgatca tgccggagcg gtacacgtac tggctgagct gctcgcggta ggcgtcgaag
     1861 tcggtgatcg gccgcgtcgc caccccggtt tccatcgccg ccttggcgac cgccaccggc
     1921 agttcgagga tcaggcgcgg atcgaagggc ttcgggatca ggtaatccgg gccgaagtgc
     1981 agcggctggt cgccgtaggc ggccatgacc gcctcgtcgg gctcctgcat ggtcaggtcg
     2041 gcgatggcgc gcacgcaggc gagcttcatc tcctcgttga tcgtgctggc gccgacatcg
     2101 agcgcgccgc ggaagatgta cgggaagcag aggacgttgt tgacctggtt cgggtagtcc
     2161 gaacggccgg tggcgaggat cgcatccggg cgcacggcct tcgccagctc cggcaggatc
     2221 tccggcgtcg ggttggcaag cgcgaggatc agcgggttcg gggccagcgt cttgaccatg
     2281 tcctgcgtca gcacgccggg gccggagacg ccgaggaaga tgtcggcacc gaccacggcc
     2341 tcgccgagcg tgcggtgcgg cacgtcggcg gcatagccgg ccttgcggtc gtccatgtag
     2401 acggtgcggc ccttgtacac gacgcccttg gagtcgcaga cgatgatgtt ctctttcttc
     2461 aggccgaggc tgaccagcag gtcgaggcag gccagtcccg ccgcaccggc gccgcagcag
     2521 gtcaggcgca ccttggtgat gtccttgccg accacgcgca ggccgttgag caccgcggcg
     2581 gtggtgatga tcgccgtgcc gtgctggtcg tcgtggaaca ccggaatctt catgcgctcg
     2641 cgcagcttct gctcgatgta gaagcactcg ggcgccttga tgtcctcaag gttgatgccg
     2701 ccgaaggtcg gctccagcga ggcgatgatg tcgatcagct tgtccgggtc gcgctcgtcg
     2761 acctcgatgt cgaagacgtt gatgccggcg aacttcttga acagcacgcc cttgccttcc
     2821 atgaccggct tcgaggccag cgcgccgatc ggcccgaggc cgagcacggc ggtgccgttg
     2881 gagatcaccg cgaccagatt gccgcgcgcg gtgacggtcg cggcttcctg cggatcctcg
     2941 acgatggcct cgcaggccgc ggccacgccg ggcgaatagg ccagcgccag atcacgctgg
     3001 ttggcgagcg gcttgctcgg caccacctcg atctttccgg gcaccggaaa gcggtggtag
     3061 tgcagcgcca tggccttgag ttcatcgctc ttcacgtctt cggacatcgg gatttcctcc
     3121 tcaagcagcg ggttgcgggc ggtgccgcgc gacaggacgg cgacacttca ttccgcagaa
     3181 acggcaaagc gccacgccgg gatcggacgt ggcgctgcgg tcacgaacgg gttgcacgga
     3241 acgacgggct gaagcggcag gcgggtcgtg tcaatagtca tgataaatcc aatcgacacc
     3301 gcacggcttg cctgaggtca gcactcgttt ccgccatccg aagcgcgacc tgcgccggtg
     3361 tggcctgacc ggtatagcaa gccgcttgcc agtgcacagg ggcacgtcac atcaagtact
     3421 tgccggtcga gacgtgcgga attccgcacg ccggccccgg tccgcggccg gaaaaccgca
     3481 cgcggccaat ggcttgcctc tgatagaatc cgcgcgccgt tttcagaagc gcactgaaag
     3541 ctgaaggacc cgccgaattc cgatccgacg cacccgccgt cgccgcagca gcaccccgga
     3601 gtcatcgatg agcacgatcc gccaggaaga tttcatccag agcattgccg acgccttcca
     3661 gtacatcagc tattaccacc cggtcgatta catccaggcg ctgtcggccg cgtatgagcg
     3721 cgaacagaat ccggctgcca aggatgcgat cgcgcagatc ctgatcaact cgcgcatgtg
     3781 cgccgaaggc caccgcccga tctgccagga caccggcatc gcgctggtct tcctgaaggt
     3841 cggcatgcac gtgaagtggg acgcgaagat gagcgtcacc gcgatggtca acgaaggcgt
     3901 gcgccgcgcc tatctgcacc ccgacaacaa gctgcgcgcc tcggtgctca ccgacccggc
     3961 aggcaagcgc acgaacagca aggacaacac cccggcggtc atccactacg agatcgtcga
     4021 cggcgatggc gtcgaggtca tctgcgcggc caagggcggc ggctccgaag ccaagtcgaa
     4081 gttcgcgatg ctgaacccct ccgacaacct ggtcgaatgg gtgctgaaga ccgtcccgac
     4141 catgggtgcc ggctggtgcc cgcccggcat cctcggcatc ggcatcggtg gcacgcccga
     4201 aaaggcgctg ctgctcgcca aggaatcgct gatggcgccg gtcgacatcc aggacctcat
     4261 cgcccgcggc ccgtcgaacc gtgccgagga actgcgcctg gagctgtacg agaaggtcaa
     4321 cgccctcggc atcggtgcgc agggtctcgg cggcctgacg accgtgctcg acgtgaaggt
     4381 gctcgactac ccgacgcacg ccgccaacct gccgatcgcg atggttccga actgcgcggc
     4441 cacccgccac gtgcacttcc acctgcacgg cgaaggcccg gcggtgctga cgccgccctc
     4501 gctcgatgac tggccgaagc tcacctacga ctccagcaag ggcaagcgcg tcaacctcga
     4561 caccgtcacg cgcgcggaag tcgcgggctg gaagccgggc gacgtgctgc ttttgaacgg
     4621 caagctgctg accggccgcg atgctgcaca caagcgcatg accgacatgc tcaaccgcgg
     4681 cgagaagctg ccggtcgact tcaacggccg cttcatctac tacgtcggcc cggtcga
//
LOCUS       DAOQIE010000292         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10461_length_4734_cov_7.359479, whole genome shotgun sequence.
ACCESSION   DAOQIE010000292 DAOQIE010000000
VERSION     DAOQIE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10461_length_4734_cov_7.359479"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..989
                     /locus_tag="PK144_15205"
     CDS             282..989
                     /locus_tag="PK144_15205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67123.1"
                     /translation="MQLFMNSAITIILLLMASPGHTAVIIDTGSNSAGTSWTFDYTQG
                     FAGEFSVGSAYTVHSIEGFFGNQGGGAGNVTISIHADGGNIPGNVLFSAPMSLAANVA
                     PDWYGLSGLGWNIGAGTYWVSFVTDLEISGSMPGNAPDPLDEYGQYSGQAWLDLGENY
                     FDYLELGIRMDGTQVQAVPEPAGLALTGFGLAALGFSRRRAKPAESLFRKSPAMEMTG
                     IGEPGRCRASGRGRHRS"
     gene            1166..>4734
                     /locus_tag="PK144_15210"
     CDS             1166..>4734
                     /locus_tag="PK144_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HNF67124.1"
                     /translation="MIRTGFTQWLQKRRVERTPADTELPLRAELYGAEQLEAHAEALA
                     RNHVVERRRTQDRLIARLQDNAAALAAGHALLAGKLDEQRSVTPAGEWLLDNYHLIEQ
                     QIRVARRNLPKNYSAKLPWLANGPGRGLPRVYDLALAVIAHGDGRVDIDALGRVVMAY
                     QRVDPLTLGELWAVPIMLQLALIENLRRVTDRLVASRRDRELALRWAVRMLEVAEREP
                     KGLILLVADMARSEPRLSSAFVAEFARNLQGHRSGGTLPLSWLAQALAESGHTIEQLV
                     EQESRLQAADQVSIGNSILTLGALSALDWREFVESVSVVEAVLAADPHGSYRRMDFAS
                     RDHYRHVVEYLADHAGGDEPAVARAALALAQDEAARHGLMARTAHIGYWLVDRGRRQL
                     EARLPLRLSPWLRLRQELARWPLTMYLGTATVTALLATGAYASGLDAEGAAPGLLGLA
                     LALAFIASSELGVTLANWLATLTVRPKPVPRLDYRHGIPPEARTLVAVPVLLGSAADI
                     DTLVDALEVRYLANRDPQLGFVLLSDLPDAATETTPDDAARIERARAGIARLNACYGR
                     ADDGNDRGSPFALLHRPRTWNAAEGVWMGRERKRGKLADLNALLLEGRTGAFSHIEGD
                     LVALAGSVRFVITLDADTQLPRDAAAAMVGALAHPLNRARFDEDSGRVVEGYGILQPR
                     VDVMPSSVHRSAYARLHGGEAGIDPYSQLASDVYQDVFGEGSFIGKGIYDVAAFEAAG
                     RSFPDNRILSHDLLEGCYARAGLLSDTQLYEDMPARHLVDARRRERWIRGDWQIGGWL
                     LARVPGADGRRRNPLSALSRWKIADNLRRSLVPAATVALLLLGAGVLGAFSGTLLLAI
                     AVLLTRPVCEALLAIVRKPPEAAPLIHLRQVGAALWRQTLLTLCRLAWLPHEAAYSLD
                     AIGRTLWRLTFSHRRLLQWTTSSDAERSTRADLAGACRQMAAGPLAAALLAAVAIPAG
                     TLPVVLPLMLMWLAGPWLAWQSGKDRPAPAPQFTAAQQALLGATSRRIWAFFETFVGP
                     QEHWLPPDNFQEQPVPALAHRTSPTNIGLALLASLSARDFGYLDTQTLIERVGGTLAT
                     LERLERLHGHFYNWYDTQSLQPLPPRYVSTVDSGNLAGHLLVLRAGLLGLAQQPFLPP
                     RLGSGLAETLLPIAPLLAPQPRAALEALAAQLPALALPEAVGRL"
BASE COUNT          669 a         1653 c         1667 g          745 t
ORIGIN      
        1 gcagcctcgc agggtgtgca gctcgctgcg caccgttcgg tacagccgtg gctggatgag
       61 tttcgttttt caaccctgtc agccatgcag ggtgcgcatt catgtgcatg cggccagaca
      121 tcaactctac ggccgtggaa acatctgatt gtcgaattct tttacatgaa gaatgactgc
      181 agatcagtca aataatcata gccatttgtt tttgaattca atttacattc tggtatgcat
      241 tgtgctctgc tgcctggctg ttcaattcga gagaaacacg catgcagctt ttcatgaatt
      301 ctgccatcac gatcatcctg cttttaatgg cctcgcccgg acacacggcg gttattatcg
      361 ataccggctc aaacagcgcc ggcaccagct ggactttcga ctatacgcaa ggctttgccg
      421 gcgagttcag tgtcggctcg gcttacacgg tgcactccat cgaaggtttc ttcgggaatc
      481 agggcggggg tgccggaaac gtgacgatca gcatccatgc ggacggcggc aacattccgg
      541 ggaacgtgct gttcagtgct ccgatgagtc tggctgcgaa tgtggctccg gactggtatg
      601 ggctgagcgg actcggctgg aatatcgggg cggggacgta ctgggtgtcc ttcgtgaccg
      661 atctggagat cagcggctcc atgcccggga atgctcccga tccgctggat gaatatgggc
      721 aatactccgg ccaggcttgg ctcgatctgg gcgagaatta tttcgattat cttgaactcg
      781 gaatccgcat ggatggaacg caagtgcagg ccgttccgga gcctgcgggt ctggcgctca
      841 cgggtttcgg gctggcagcc ctggggtttt cacgccgcag ggcaaagccg gcggaaagct
      901 tgttccggaa atcgccggcc atggagatga ccggcatcgg cgagcccggt cgctgccgcg
      961 catcggggcg aggcaggcac cggtcctgag gagccctggc gcaggcggcg gcaccgcgaa
     1021 gccgcctgct gccctgccgg cgcacgcccc ggagcgcgcc ggcatgtcat gaaaccggaa
     1081 cgggccggaa ccgcgctgtg gcgcggccgg tctttcgttt ccggcggtga cgttccgttg
     1141 cccgcgcacc tgggggatgg aatgcatgat caggaccggg ttcacgcagt ggctgcagaa
     1201 gcgccgggtc gaacgcacgc cggccgatac cgagctgccc ctgcgcgccg agctttacgg
     1261 cgccgagcag cttgaagccc acgccgaggc gctggcgcgc aatcacgtcg tcgagcgccg
     1321 gcgcacgcag gaccggctga tcgcgcggct gcaggacaac gccgcggcgc tcgccgccgg
     1381 gcatgcgctg ctcgccggca agctcgacga gcagcgcagc gtcacgccgg ccggcgaatg
     1441 gctgctcgac aactaccacc tgatcgagca gcagatccgc gtcgcgcgcc gcaacctgcc
     1501 gaagaactac agcgccaagc tgccgtggct cgccaacggt cccggccgcg ggctgccgcg
     1561 cgtctatgac ctggcgctgg ccgtcatcgc gcacggcgac ggccgcgtcg acatcgacgc
     1621 gctcgggcgc gtggtgatgg cctaccagcg ggtcgatccg ctgacgctcg gcgagctgtg
     1681 ggccgtgccg atcatgctgc agctcgcgct gatcgagaac ctgcgccgcg tcaccgaccg
     1741 cctggtcgcc agtcgccgcg accgcgaact ggcgctgcgc tgggccgtgc gcatgctgga
     1801 ggtcgccgag cgcgagccga aggggctgat cctgctcgtc gccgacatgg cgcgctccga
     1861 accgcggctc tccagcgcct tcgtcgccga gttcgcgcgc aacctgcagg gccaccgcag
     1921 cggcggcacg ctgccgctga gctggctcgc gcaggcgctg gccgagtccg ggcacacgat
     1981 cgagcagctc gtcgagcagg agagccggct gcaggcggcc gatcaggtgt cgatcggcaa
     2041 cagcatcctg acgctcggtg cgctgagtgc gctcgactgg cgcgagttcg tcgaatcggt
     2101 cagcgtcgtc gaggcggtgc tcgccgccga tccgcacggc agctaccggc gcatggactt
     2161 cgcgagccgc gaccattacc ggcacgtcgt cgaatacctc gccgatcacg ccggcggcga
     2221 cgagccggcc gtggcgcggg cggcgctggc gctggcgcag gacgaggccg cgcgccacgg
     2281 gctgatggcg cgcaccgccc acatcggtta ctggctggtg gaccgcggcc ggcgccagct
     2341 cgaagcccgg ctgccgctgc ggctgtcgcc atggctgcgg ctgcggcagg agctggcgcg
     2401 ctggccgctg acgatgtacc tcggcaccgc caccgtcacc gcgctgctcg cgaccggcgc
     2461 ctacgcaagt gggctcgatg ccgagggtgc cgcccccggc ctgctgggcc tggcgctcgc
     2521 gctcgccttc atcgccagca gcgagctggg cgtgaccctc gccaactggc tcgcgacgct
     2581 gacggtgcgc ccgaagccgg tgccgcggct cgattaccgc catggcatcc cgcccgaggc
     2641 gcgcacgctg gtggcggtgc cggtgctgct cggcagcgct gcggacatcg acacgctcgt
     2701 cgatgcgctg gaggtgcgct acctcgccaa ccgcgatccg cagctcggct tcgtgctgct
     2761 gtcggacctg cccgatgccg cgaccgaaac gacgccggac gatgccgcgc gcatcgagcg
     2821 tgcgcgcgcc ggcattgccc ggctcaacgc gtgctacggc cgtgccgatg acggcaacga
     2881 ccgcggcagc ccgttcgcgc tgctgcaccg gccgcgcacg tggaacgcgg ccgaaggcgt
     2941 gtggatgggg cgcgagcgca agcgcggcaa gctcgccgat ctcaacgcgc tgctgctcga
     3001 aggccgcacc ggggccttct cgcacatcga gggcgacctc gtggcgctcg ccggcagcgt
     3061 gcgcttcgtc atcacgctcg atgccgacac gcagctgccg cgcgatgcgg ccgcggcgat
     3121 ggtcggcgcg ctcgcccacc cgctgaaccg cgcccgcttc gacgaggaca gcggccgcgt
     3181 cgtcgagggc tacggcatcc tgcagccgcg cgtcgacgtg atgccttcga gcgtgcaccg
     3241 ctcggcgtat gcgcggctgc acggcggcga ggccggcatc gacccgtaca gccagctcgc
     3301 ctccgacgtg tatcaggatg tcttcggcga aggctccttc atcggcaagg gcatctatga
     3361 cgtggccgcg ttcgaggcgg ccgggcgcag ttttccggac aaccgcatcc tcagccacga
     3421 cctgctcgaa ggctgttacg cgcgcgcggg gctgctgagc gacacgcagc tctacgagga
     3481 catgccggcg cgccacctcg tcgatgcccg gcggcgcgag cgctggatcc gcggcgactg
     3541 gcagatcggc ggctggctgc tggcgcgcgt gccgggagcg gacgggcggc ggcgcaatcc
     3601 gctcagtgcg ctgtcgcgct ggaagatcgc cgacaacctg cgccgcagcc tggtgccggc
     3661 ggcgaccgtg gcgctgctgc tgctcggggc cggggtgctc ggggccttca gcggcacgct
     3721 gctgctggcg atcgccgtgc tgctgacgcg cccggtgtgc gaggcgctgc tcgcgatcgt
     3781 gcgcaagccc ccggaggccg cgccgctgat ccacctgcgc caggtcgggg ccgcgctgtg
     3841 gcgccagacg ctgctgacgc tctgccggct ggcgtggctg ccgcacgagg ccgcctacag
     3901 cctcgatgcg atcggccgca cgctgtggcg gctcacgttc agtcaccgcc ggctgctgca
     3961 gtggacgacc tccagcgatg ccgagcgcag cacccgcgcc gatctcgccg gcgcctgccg
     4021 gcagatggcg gccgggccgc tcgccgctgc gctgctggcg gccgtggcga tcccggccgg
     4081 cacgctgccg gtggtgctgc cgctgatgct gatgtggctc gccggcccgt ggctggcgtg
     4141 gcagagcggc aaggaccggc cggcgccggc gccgcagttc acggccgccc agcaggcgct
     4201 gctcggcgcc acgtcgcggc gcatctgggc gttcttcgag accttcgtcg gaccgcagga
     4261 gcactggctg ccgccggaca acttccagga gcagccggtg ccggcgctcg cgcaccgcac
     4321 ctcgccgacc aacatcggtc tggcgctgct cgccagcctg tcggcgcgcg atttcggcta
     4381 tctcgacacg cagacgctga tcgagcgcgt cggcggcacg ctggcgacgc ttgagcgcct
     4441 cgaacggctg cacgggcatt tctacaactg gtacgacacg cagagcctgc agccgctgcc
     4501 gccgcggtat gtatcgaccg tcgacagcgg caacctcgcc ggccacctgc tggtgctgcg
     4561 cgccgggctg ctcgggctgg cgcagcagcc cttcctgccg ccgcgcctcg gcagcggcct
     4621 ggccgagacg ctgctgccga tcgcaccgct gctggcgccg cagccgcgtg cggcgctgga
     4681 ggcgctcgcc gcgcagctgc cggcgctcgc gctgcccgag gccgtcggcc ggct
//
LOCUS       DAOQIE010000293         4722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10504_length_4722_cov_5.693593, whole genome shotgun sequence.
ACCESSION   DAOQIE010000293 DAOQIE010000000
VERSION     DAOQIE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10504_length_4722_cov_5.693593"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PK144_15215"
     CDS             <1..1201
                     /locus_tag="PK144_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNF67125.1"
                     /translation="GLAKQLAGAARSAEPAGGGGGAVSIEAEENSNALLLTAPPTALR
                     SLLEVIAQLDVRRAQVLVEAIVAEISLERSAKLGVQWLIGDRQGETVVGIVNFDNLLN
                     TLAGAVAAREIPQSLASGAHVAVSAGAGSLRFAALISALSSDGNTNVLSTPSLVTLDN
                     QEAEIIVGQNVPFVTGTFTTSVGGSLGAGTGLGSGSSGAALGLVGNPFQTIQRQDVGL
                     QLKVKPQINENSAIRLDISQEVSSLASVSPSQGPTTNKRSIKTSVMIEDGQLLVLGGL
                     IDDSVNDAEDKVPVLGDVPVLGGLFRYRNQKKVKRNLMIFLRPVILRDGVAATAGRYA
                     AIRGVQQQAAAVSDTPSAVLPPFDEPYPGASLALGIGRRLSFFGDTTVFGWDDPAVRA
                     FDASLER"
     gene            1209..2684
                     /gene="gspE"
                     /locus_tag="PK144_15220"
     CDS             1209..2684
                     /gene="gspE"
                     /locus_tag="PK144_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNF67126.1"
                     /translation="MNAPDLPYPFAKRHGVLVLERDARAVTVGLRAKPAAAVLMEARR
                     VLGLPLRLQVLTTTEFDARLAARYSQAADGAARMIEDICETDLARVAQALPEPEDLLE
                     SADDAPIIRLINALLTEAIREHASDIHIECFETRLNVRLRVDGVLRDVLAPPRALGPL
                     LVSRIKVMANLDIAEKRLPQDGRISLRVAGHAVDVRVSTIPSGHGERVVLRLLDKREG
                     RIELSALGLGAQASALLDRLIHRPHGILLVTGPTGSGKTTTLYSALTRINETSRNIMT
                     VEDPIEYYLDGVSQTQVNTRIDMSFARGLRALLRQDPDVIMVGEIRDPETADIAIQAS
                     MTGHLVLSTLHTNSAVGAIARLRDMGVEPFLLASSLIGVLAQRLVRRLCPHCRQPVAA
                     GAAERRELEVPGTAALQLQVACGCPQCRSSGYRGRVGLFELVVIDEALRAAIHAGASE
                     AELEALARTQGPSLRADGVARVLAGDTSLAELLRVTRELSA"
     gene            2681..3889
                     /gene="gspF"
                     /locus_tag="PK144_15225"
     CDS             2681..3889
                     /gene="gspF"
                     /locus_tag="PK144_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018175226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNF67127.1"
                     /translation="MNAYGYRALDAGGRTRKGAIEADTPRAARSLLRERGLIALELQP
                     LASSGAQQGGRPARGLAPTELALMTRQLATLIDSGLPVEQALAAVAAGLGQGRVAGLV
                     LAVRAGITEGRSLADALGDFPRAFPDLYVATVAAGERSGLLAPVFERLAEYTEARQQL
                     RQKIALALFYPLLLGCVATGVVLVLLTWVVPQVTGVFASLHQELPPLTRGLIAVADFL
                     RARGWMLGAVLLAGLIGLLRLRRIGAVREWLDAQYLRLPLFGRLQRALDAARCMRTFS
                     ILITSGVSMLEALRIAAQVVSSVPIRHSLLLAAVRVREGASLSSALRDSGQFPAMTVQ
                     LLASGEASGRLGVMAERAACQQEREAEMRLAALLALFEPVLILTLGGIVLLIVLAILL
                     PIFDLNQLVH"
     gene            3901..4353
                     /gene="gspG"
                     /locus_tag="PK144_15230"
     CDS             3901..4353
                     /gene="gspG"
                     /locus_tag="PK144_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNF67128.1"
                     /translation="MSPASRMRARGLQRGFTLIEIMVVVVILGVLAAIVVPKIMDRPD
                     QARVVRARQDLRTLASALALYRLDNYAYPSTTQGLEALTARPVDPPLPPHWKAGGYLE
                     NLPRDPWGHPYLYLSPGRHGEFDLWSHGADGQPGGEGFNADLGSWDVN"
     gene            4343..>4722
                     /locus_tag="PK144_15235"
     CDS             4343..>4722
                     /locus_tag="PK144_15235"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNF67129.1"
                     /translation="MSTEARRPAAGYTLLEVMIVVTIIGLLSALAVLRLPGSDAPARA
                     LAAAGRLAIGFAELRDEARLTGMARGMGCQAQLCQGFRPDDSGRHWQPLSGPGGEPLR
                     IVLEEGLTVAFDPRLSPEAPAPALR"
BASE COUNT          636 a         1669 c         1686 g          731 t
ORIGIN      
        1 gggcctggcc aaacagctgg ccggtgcggc ccgcagcgcc gagccggcgg gcggcggcgg
       61 cggggcggtc agtatcgagg ccgaggagaa ctccaatgcg ctgctgctga cggcaccgcc
      121 aacggcgctg cgctcgctgc tcgaagtcat cgcgcaactc gacgtgcgcc gcgcccaggt
      181 gctggtcgag gccatcgtcg ccgagatctc gctcgaacgc tccgccaagc tcggcgtgca
      241 atggctgatc ggcgaccgtc agggcgagac cgtcgtcggc atcgtcaatt tcgacaatct
      301 gctgaacacg ctggccgggg cggtcgcggc gcgcgagatt ccgcagtcgc tggcgtcggg
      361 ggcacacgtg gcggtgtcgg ccggcgcggg ttcgctgcgc ttcgcagccc tgatcagtgc
      421 gctgagcagc gacggcaaca ccaacgtgct gtccaccccc agcctggtga cgctcgacaa
      481 ccaggaggcc gagatcatcg tcggtcagaa cgtgccgttc gtgaccggga ccttcacgac
      541 cagcgtcggt ggctcgctgg gcgcgggcac cggactcggc tccggcagca gcggtgctgc
      601 gctcgggctg gtcggcaacc cgttccagac gatccagcgc caggacgtcg gcctgcagct
      661 gaaggtcaag ccgcagatca acgagaacag cgccatccgg ctcgacatca gtcaggaggt
      721 gtcgagtctg gcctcggttt cgccttcgca ggggccgacc accaacaagc gttcgatcaa
      781 gaccagcgtc atgatcgagg atggccagct gctggtgctc ggcggcctga tcgacgacag
      841 cgtcaatgat gccgaggaca aggtgccggt gctgggcgac gtgccggtgc tcggggggct
      901 gttccgctac cgcaaccaga agaaggtcaa gcgcaacctg atgatcttcc tgcggccggt
      961 gatcctgcgc gacggggtgg ccgctaccgc cggccgatac gccgcgatcc gcggcgtcca
     1021 gcagcaggcg gccgccgtca gcgacactcc atcggcggtg ctgcccccgt tcgatgagcc
     1081 ttatcccggg gcctcgctgg cgcttggcat cgggcgccgg ctgagtttct tcggcgacac
     1141 gacggtgttc ggctgggacg atccggccgt gcgtgccttc gatgccagcc tcgaacgctg
     1201 aatgtgcgat gaacgctccg gatctgccct atccgtttgc caagcgccac ggcgtcctcg
     1261 tgctggagcg tgacgcgcgg gcggtcaccg tcggactgcg ggcaaaaccc gcggcagccg
     1321 tgctgatgga agcgcgccgc gtgctcggcc tgcccctgcg gctgcaggtg ctgacgacga
     1381 cggagttcga tgcgcggctc gccgctcgct actcgcaggc cgcggacggg gcggcccgca
     1441 tgatcgagga catctgcgag accgacctgg ctcgcgtcgc ccaggcgctg cccgagcccg
     1501 aggacctgct cgaatcggcg gacgatgcgc cgatcatccg gctgatcaac gccttgctga
     1561 ccgaggcgat ccgcgagcac gcgtccgaca tccacatcga gtgcttcgag acgcggctga
     1621 acgtgcgcct gcgcgtcgat ggcgtgctgc gcgatgtcct ggcgcctccg cgcgctctgg
     1681 ggccgctgct ggtgtcgcgg atcaaggtca tggcgaatct cgacatcgcc gaaaagcgtc
     1741 tgccgcagga cggccgcatc tcgctgcgcg ttgccggcca cgccgtcgac gtgcgcgtct
     1801 cgacgattcc ctcggggcac ggggagcgcg tcgtgctgcg tctgctcgac aagcgcgagg
     1861 ggcgcatcga gctgtcggcg ctcggtctcg gggcgcaggc ctcggcgctg ctcgaccggc
     1921 tgatccaccg tccgcacggc atcctgctcg tgaccggccc gaccggctcc ggcaagacga
     1981 ccacgctcta cagcgccctg acgcgcatca acgagaccag ccgcaacatc atgacggtcg
     2041 aggatccgat cgagtactac ctcgacggcg tcagccagac ccaggtcaac acgcgcatcg
     2101 acatgagttt tgcccgcggg ctgcgtgcgc tgctgcgcca ggatccggac gtcatcatgg
     2161 tgggcgagat ccgcgatccg gaaaccgccg acatcgccat ccaggcgtcg atgaccgggc
     2221 atctggtgct ctcgacgctg cacacgaatt ccgccgtcgg tgccatcgcg cggctgcgcg
     2281 acatgggcgt cgaacccttc ctgctcgcct cgtcgctgat cggggtgctg gcgcagcggc
     2341 tggtgcgccg gctctgcccg cactgccggc agccggttgc ggccggggcc gccgagcggc
     2401 gcgagctcga agtccccggc accgcagcgc tgcagctgca ggttgcctgc ggctgcccgc
     2461 agtgccgctc cagcggctat cgcggacgcg tcgggctgtt cgagctcgtc gtcatcgatg
     2521 aggcgctgcg cgccgccatc catgcaggcg cgagcgaggc ggagcttgaa gccctggcgc
     2581 gcacccaggg gccgtcgctg cgtgccgacg gggtggcgcg cgtgctggcc ggcgacacct
     2641 cgctggcgga actgctgcgc gtcacgcgcg agctgtcggc atgaacgcct acggctaccg
     2701 ggcgctcgat gccggcgggc gcacgcgaaa gggcgcgatc gaggccgaca cgccgcgtgc
     2761 cgcccgcagc ctgctgcgcg agcgcgggct gatcgcgctc gaactgcagc cgctcgcgag
     2821 cagcggcgcg cagcagggcg gacgtccggc ccgggggctg gccccgaccg aactcgccct
     2881 gatgacccgg cagctggcca cgctgatcga ctccgggctg ccggtcgagc aggcccttgc
     2941 cgcggtcgct gccggactcg gccagggccg ggtcgccggg ctcgtgctcg cggtgcgggc
     3001 cggcatcacc gaggggcgtt cgctcgccga tgcgctgggc gacttcccgc gggcgtttcc
     3061 cgatctctac gtcgccacgg tcgccgccgg cgagcgatcc ggcctgctcg caccggtatt
     3121 cgaacgtctg gccgagtaca ccgaagcgcg ccagcagctg cgccagaaaa tcgcactcgc
     3181 gctgttttat ccgctgctgc tcggctgcgt cgccaccggg gtcgtgctgg tgctgctgac
     3241 ctgggtggtg ccgcaggtca ccggtgtgtt cgcctcgctg caccaggagc tgccgccgct
     3301 gacgcgcggg ctgatcgcgg ttgcggactt cctgcgcgcc cggggctgga tgctgggcgc
     3361 cgtcctgctg gccgggctga tcggcctgct gcgcctgcgc cggatcggtg cggtgcgcga
     3421 gtggcttgat gcgcagtatc tgcgcctgcc gctgttcggc cgcctgcagc gcgcactcga
     3481 tgccgcccgc tgcatgcgca ccttcagcat cctgatcacc agcggcgtgt cgatgctcga
     3541 agccctgcgc atcgcagcgc aggtcgtcag cagcgtgccg atccgccatt ccctgctgct
     3601 ggcggccgtg cgcgtgcgcg agggcgcgag cctcagcagc gcgctgcgcg acagcggcca
     3661 gttcccggcg atgaccgtgc agctgctggc cagcggcgag gccagcggcc ggctcggcgt
     3721 catggccgag cgggccgcgt gccagcagga gcgcgaggcg gagatgcgcc tcgccgcctt
     3781 gctcgccctg ttcgagccgg tgctgatcct gacgctcggc ggcatcgtgc tgctgatcgt
     3841 gctcgcgatc ctgctgccga tcttcgacct caaccagctg gttcactaga caggaccgcc
     3901 atgtcccctg catcacgcat gcgtgcccgc ggccttcagc gcggcttcac cctcatcgag
     3961 atcatggtcg tcgtcgtcat cctcggggtg ctggcggcga tcgtcgtgcc gaaaatcatg
     4021 gaccggcccg atcaggcgcg cgtggtccgg gcgcgccagg atctgcgcac gctcgcgagc
     4081 gcgctggcgc tgtaccggct cgacaattac gcgtatccga gcaccaccca ggggctggag
     4141 gcgctgaccg cccgcccggt cgatccgccg ctgccgcccc actggaaggc cggcggctac
     4201 ctcgagaatc tgccccggga cccgtgggga cacccctacc tgtacctgtc cccgggccgg
     4261 cacggcgagt tcgacctctg gtcgcacggg gccgacggcc agccgggcgg ggaaggcttc
     4321 aatgccgatc tcggcagctg ggatgtcaac tgaggcgcgg cggccggcgg ccggctacac
     4381 gctgctcgaa gtcatgatcg tcgtgacgat catcggcctc ctgtcggcgc tggccgtgct
     4441 gcgcctgccc ggcagcgatg cgccggcgcg ggccctggcc gctgccggac ggctcgcgat
     4501 cgggttcgcg gagctgcgcg acgaggcgag actcaccggc atggcgcggg gcatgggctg
     4561 tcaggcgcag ctctgccagg gcttccgtcc ggatgacagc ggccggcact ggcagccgct
     4621 gtccggcccg ggcggcgagc ccctgcggat cgtgctggag gaagggctga cggtggcctt
     4681 cgatccgcgc ctgagccccg aagcgccggc gccggcgctt cg
//
LOCUS       DAOQIE010000294         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10605_length_4692_cov_4.562217, whole genome shotgun sequence.
ACCESSION   DAOQIE010000294 DAOQIE010000000
VERSION     DAOQIE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_4692_cov_4.562217"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK144_15240"
     CDS             <1..480
                     /locus_tag="PK144_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNF67130.1"
                     /translation="RALRERIGLFQSFVGRLQPILARLPRAIETATLGGAQAREHATA
                     DVLAEAERLRSAAPDGFDLDEITAELSEPLTRPAATYDLDWLKRNLLEKPDRLPPGAA
                     AQRLGDADFAYLRPGLAGSVRVTTDAAYYEEHADSVELWSPGSPLFPRLGEDGNASA"
     gene            553..4341
                     /locus_tag="PK144_15245"
     CDS             553..4341
                     /locus_tag="PK144_15245"
                     /inference="COORDINATES: protein motif:HMM:NF023550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF67131.1"
                     /translation="MEAAPESADFALLRLILIDAWSPGRIVELPLVGGTVLTGRNGRG
                     KTSLLQLLPLFYGERPSAIASAEANRLGFVAYYLPRSTSYLVYEYVRQRDQRRCVIVH
                     ADRSGEQLRYRFLRGPYREDCFVLGNGELVAAPDLRRHIELLGAEVSEQIDSTAEYRA
                     LIQGLPPATRDRERRQVLVRLGADYALAPRHVRLPNLEKIVSGMFRRRTHFEELQGMV
                     IDCVAERDAAPELGGERARVEAWPRDYRAYRDVMAQAARMDEAQRLHDELLAVEAGLG
                     ELHARHLSLIDASLAQHEDCRRSQAALAATRAEAGRAHDAERRALEDELHAVRRTRED
                     ARARIERLDAQQREYAQAGIERLREQVARGPALREQAQLLERRREALLGEQQAISGRY
                     ERLELEQQKAGLQAREGFAAERRQIEQAHATAVQAAESGHAGSEAARRSAQRDEAAAL
                     NARLEAAADALGRCSARCEQPPVDAASEQLVAGRRLAVDRLLQDRDGALEREHAAARA
                     MTQAAQMYSDAERAQAQARHALEAAGREVEARAAWCTPAPDTLLAFLRAEHPDWSEDV
                     ARVLREDLLLRTDLAPQAERDGNGGGLYGIGLALDRLEPLAAADPDGARQGLASAQAQ
                     AAEARKVLAEADRALETAAAARRKAQQAAREHEAERRKLDAHIELARRELKTAQDQRE
                     QALAAARERAEAERQAALTAHAALRGERERLIAAQQQADRGAADAHAGRLQLLRDQRD
                     AALAGLGAREREDETGRAQALAVLLAERDRALQAGGVDTEALRALDAELRELQQRLQL
                     AREAQGKVREWQRWHDEDWPKRAQAAAAETEADAKLATLRAQLDAQAGRWRERLAAFE
                     AQAAALAARQTELDRTLAGARARCQSTALGSFPAGGTPPPWQPEWTLDALGVALQPAL
                     SRQRRLLKALDEQVLPIQKTFAAQTDAPPQQYFEQHRSELPATAAARDWVAPLAAWYA
                     GEHLSYRRILMQEAGAIRAIVADFHGRMSAFHRRVQQFNRELKQSLDTGLAFDSITRI
                     EVEVISTLRELAYWPAIEALVEAPQARAGDGAVELPPPEFASTIGQLLEHWEVRSGIR
                     AELRELIRIQGEVEENGQRRAFRRAADLEKVSSNGLSYLVLCSIFVAFINRIRRQAPV
                     DVVWALDELKDLDAGNVARLLELLARNRITLVSAFPDPDVETLRLFRHRFGIEPDRRL
                     AEVRILDAAPLPAPEAGPAVAAAADVEPEPEPEPEPEAEAEAEAEAEAAVPAGDRADV
                     "
     gene            4334..>4692
                     /locus_tag="PK144_15250"
     CDS             4334..>4692
                     /locus_tag="PK144_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67132.1"
                     /translation="MFETVARRLLAGDFLCPYASPELHAWLLDEAARAEIDAWLARIG
                     LRLALTRQGTAFYAAHAGIGSEERREAAEAFREVQQQLRPMVNFLVLAMRATQDDSVL
                     MPGSLLEAAALHAAIDA"
BASE COUNT          676 a         1678 c         1771 g          567 t
ORIGIN      
        1 cgggcactgc gcgagcggat cgggctgttc cagagtttcg tcgggcgcct gcagccgatt
       61 ctggccagac tgccgcgggc gatcgaaacc gcgacgctgg gcggtgcgca ggcacgcgag
      121 cacgcgacgg ccgatgtgct tgccgaagct gagcgcttgc gcagcgcggc accggatggt
      181 ttcgatctgg acgagatcac ggccgaactg tccgaaccgc tcacgcggcc ggcggctacc
      241 tatgacctcg actggctgaa acggaacctg ctggaaaagc cggaccgtct gccaccggga
      301 gccgcagccc agcggctcgg tgacgcggat ttcgcctatc tgcgacccgg ccttgcaggc
      361 agtgtccgcg tcaccaccga tgcggcctac tacgaggaac atgccgacag cgtcgaactg
      421 tggtcgccgg gcagtccgct gttcccgagg ctgggcgagg acggtaacgc cagcgcatga
      481 gccggtgacc gccgtgaagt gcgatcatcc ccggttcgcc ccggccgtcc gccgaagcca
      541 tgcgccgccc ccatggaagc tgctcccgaa tccgccgatt tcgcgctgct gcgtctgatc
      601 ctgatcgatg cctggagtcc cgggcgcatc gtcgagctgc cgcttgtcgg cggcacggtg
      661 ctgaccgggc gcaacgggcg cggcaagacc tcgctgctgc agctgctgcc gctgttctac
      721 ggcgagcggc cgtcggcgat tgccagtgcc gaggccaacc ggctcggctt cgttgcctac
      781 tacctgccgc gcagcacctc gtacctcgtc tacgaatacg tgcgccagcg cgaccagcgc
      841 cgctgcgtga tcgtgcatgc cgatcgcagc ggcgagcagt tgcgctaccg cttcctgcgc
      901 gggccgtatc gcgaggactg cttcgtgctc ggcaacggcg agctggtcgc ggcgccggat
      961 ctgcgccggc acatcgagct gctcggtgcc gaggtcagcg agcagatcga ctcgacggcc
     1021 gagtaccgcg cgctgatcca gggcctgccg ccggcgacgc gcgaccgcga gcggcggcag
     1081 gtgctggtgc gtctcggggc cgactacgcg ctggcgccgc gccacgtgcg gctgccgaac
     1141 ctcgaaaaga tcgtcagcgg catgttccgc cggcgcacgc acttcgagga gctgcagggc
     1201 atggtcatcg actgcgtcgc cgagcgtgac gcggcgcccg aactcggcgg cgagcgcgcg
     1261 cgcgtcgagg cctggccgcg cgattaccgc gcctaccgcg acgtgatggc gcaggccgcg
     1321 cgcatggacg aggcccagcg cctgcacgac gagctgctgg cggtcgaggc cggactcggc
     1381 gagctgcacg cgcgccacct gagcctgatc gatgcgagcc tcgcgcagca cgaggactgc
     1441 cggcgctcgc aggcggcgct cgcggcgacg cgcgccgagg cgggccgtgc ccacgacgcc
     1501 gagcgccgcg ccctcgaaga cgagctgcac gccgtgcggc gcacgcgcga ggacgcccgg
     1561 gcgcgcatcg agcggctcga tgcgcagcag cgcgagtacg cgcaggccgg catcgagcgc
     1621 ctgcgcgaac aggtcgcgcg cgggccggcg ctgcgcgagc aggcgcagtt gctcgaacgc
     1681 cggcgcgagg cgctgctcgg cgagcagcag gcgatctcgg ggcgctacga gcggctggaa
     1741 ctcgaacagc agaaggccgg gctgcaggcg cgcgagggct tcgcggccga gcgccggcag
     1801 atcgagcagg cgcatgcgac ggcggtgcag gcggcggaat ccggccacgc cggctccgaa
     1861 gccgcccgcc gcagcgcgca gcgtgacgag gcggcggcgc tgaacgcccg gctcgaagcc
     1921 gcggccgatg cgctcgggcg ctgcagcgcg cgctgcgagc agccgccggt cgatgcggcg
     1981 agcgagcagc tcgtcgccgg gcggcggctg gccgtcgacc ggctgctgca ggatcgggac
     2041 ggcgcgctcg aacgcgagca cgctgccgcc cgggccatga cgcaggccgc gcagatgtac
     2101 tccgatgccg agcgcgcgca ggcgcaggcg cggcacgcgc tggaggccgc cgggcgcgag
     2161 gtcgaggcgc gtgcggcgtg gtgcacgccg gcgccggaca cgctgctcgc cttcctgcgc
     2221 gccgagcatc cggactggtc cgaagatgta gcgcgcgtgc tgcgcgagga tctgttgctg
     2281 cgcaccgatc tcgcgccgca ggccgagcgg gacggcaatg gcggcgggct gtacggcatc
     2341 gggctggcgc tcgatcggct cgaaccgctg gcggcggccg atcccgacgg cgcgcgtcag
     2401 gggctggcga gcgcgcaggc gcaggccgcg gaagcccgca aggtgctggc cgaggccgat
     2461 cgcgcgctcg aaaccgctgc cgcggcacgc cgcaaggcgc agcaggcggc gcgcgagcac
     2521 gaggcggagc ggcgcaagct cgatgcgcac atcgagcttg cgcgtcgcga actgaagacc
     2581 gcgcaggacc agcgcgaaca ggcgctcgcc gctgcccgcg agcgcgccga agccgaacgg
     2641 caggcggccc tgacggcgca tgcggcgctg cgcggcgagc gcgaacggct catcgctgcg
     2701 cagcagcagg cggatcgcgg cgccgccgac gcgcacgccg gccggctgca gcttctgcgc
     2761 gatcagcgtg acgccgcact cgccggcctc ggggcacgcg agcgcgagga cgagaccggg
     2821 cgcgcgcagg cactggccgt cctgctcgcg gagcgcgacc gggcgctgca ggccggcggc
     2881 gtcgataccg aagccctgag ggcgctcgat gccgaactgc gcgagctgca gcagcgcctg
     2941 cagcttgcgc gcgaggcgca gggcaaggtg cgcgaatggc agcgctggca tgacgaggac
     3001 tggccgaagc gcgcgcaggc cgcggccgcc gaaaccgaag cggatgcaaa gctcgccacg
     3061 ctgcgcgcgc aactcgatgc gcaggccggg cgctggcgcg aacggctcgc ggccttcgag
     3121 gcgcaggccg cggcgctggc cgcgcggcag accgagctgg atcgcacgct ggccggcgcc
     3181 cgcgcgcgct gtcagagcac cgcgctcggc agcttcccgg ccggcgggac gccgcctccc
     3241 tggcagccgg aatggacgct cgatgcgctc ggcgtggcgc tgcagccggc gctgagccgc
     3301 cagcgccggc tgctgaaggc gctcgacgag caggtgctgc caatccagaa gaccttcgcg
     3361 gcgcagaccg atgcgccgcc gcagcagtac ttcgagcagc accgcagcga gctgccggcg
     3421 acggcggcgg cgcgcgactg ggtcgcaccg ctcgccgcct ggtacgccgg cgagcacctg
     3481 agctaccggc gcatcctgat gcaggaggcc ggggcgatcc gcgccatcgt cgccgacttc
     3541 cacggccgga tgtcggcctt ccaccggcgc gtgcagcagt tcaaccgcga gctgaagcag
     3601 agcctcgaca ccgggctcgc cttcgacagc atcacgcgca tcgaggtcga ggtgatctcg
     3661 acgctgcgcg agcttgccta ctggccggcg atcgaggcgc tggtcgaggc gccgcaggcg
     3721 cgcgccggcg acggcgccgt cgagctgccg ccgccggagt tcgcgagcac gatcggccag
     3781 ctgctcgaac actgggaggt gcgctccggc atccgcgccg agctgcgcga gctgatccgc
     3841 atccagggcg aggtcgagga gaacggccag cgccgggcgt tccggcgcgc ggccgatctc
     3901 gaaaaggtgt cgtcgaacgg cctgtcgtat ctggtgctct gcagcatctt cgtcgccttc
     3961 atcaaccgca tccgccggca ggcgccggtc gatgtggtct gggcgctcga cgagctgaag
     4021 gacctcgacg ccggcaacgt cgcgcgcctg ctcgaactgc tggcgcgcaa ccgcatcacg
     4081 ctggtctcgg ccttcccgga cccggatgtc gagacgctgc gcctgttccg ccaccgcttc
     4141 ggcatcgaac ccgaccggcg gctggccgag gtgcgcatcc tcgatgccgc gccgctgccg
     4201 gcgcccgaag ccgggccggc cgtggcagcg gcggccgatg tcgagcctga acccgagcct
     4261 gaacccgagc ccgaagcgga agccgaagcg gaagccgaag cggaagccgc cgtgccggcc
     4321 ggagatcgcg ccgatgtttg agaccgttgc ccgccggctg ctggccggcg atttcctctg
     4381 tccgtacgcg agccccgagc tgcacgcctg gctgctcgat gaggcggcgc gcgccgagat
     4441 cgatgcctgg ctcgcgcgca tcggcctgcg gctggcgctc acgcgccagg gcacggcctt
     4501 ctacgccgcg cacgccggca tcggcagcga ggagcggcgc gaggcggccg aggcgtttcg
     4561 cgaggtgcag cagcagttgc ggccgatggt gaacttcctg gtgctggcga tgcgcgcgac
     4621 ccaggacgac agcgtgctga tgccgggcag cctgctcgaa gccgcggcgc tgcacgcggc
     4681 gatcgacgcc ag
//
LOCUS       DAOQIE010000295         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10692_length_4662_cov_6.898198, whole genome shotgun sequence.
ACCESSION   DAOQIE010000295 DAOQIE010000000
VERSION     DAOQIE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10692_length_4662_cov_6.898198"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PK144_15255"
     CDS             complement(<1..183)
                     /locus_tag="PK144_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNF67133.1"
                     /translation="MSDQPPPDPWKEPASPPPAPGTAAPAPVEASALAVLADRARELL
                     RERRRERRWRLFFRATW"
     gene            complement(206..880)
                     /locus_tag="PK144_15260"
     CDS             complement(206..880)
                     /locus_tag="PK144_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNF67134.1"
                     /translation="MPDRAFDLLVFDWDGTLMDSEYRIVACLRAASLSLGLPALADAA
                     YRHVIGLGLIEACAMLYPLAGDDEHLRLAEAYKHEFLSRDPAGMQLFAGAAGVLEALC
                     ADGYRLAVATGKSRAGLERAYRDTGLGRLFEASRCADETASKPDPRMLHELLAETGVP
                     AARALMIGDTEFDLAMARAAGMAGIGATWGCHAPELLLRHAPLACLDDIADLPALLAS
                     LPAAAA"
     gene            complement(912..1862)
                     /locus_tag="PK144_15265"
     CDS             complement(912..1862)
                     /locus_tag="PK144_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF67135.1"
                     /translation="MSQSSPPPAVRFVEVPAECDGQRIDNFLIRTLKGVPKSLVYRIL
                     RTGEVRVNKGRARPEHRVAAGDSVRLPPIRVAAEDDAPAMPARGLLERLEAAILYEDA
                     RLLVLDKPSGIAVHAGSGIDAGVIEGLRVLRPEVFLELAHRLDRDTSGVLVLAKTPET
                     LRAIHDALNTRSTDKRYLALVRGAWRGGEHVVDAPLRKGMIRGGERMVEVAPDGKPAL
                     SRFRPLRRHADCTLVEVTIETGRTHQIRVHAAHLGHPLAGDDKYGDPAFNRTLAALGL
                     RRLFLHAQLLSLPLGGRPLTLEAPLCPELARVLDTLAARG"
     gene            2282..>4662
                     /gene="rne"
                     /locus_tag="PK144_15270"
     CDS             2282..>4662
                     /gene="rne"
                     /locus_tag="PK144_15270"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HNF67136.1"
                     /translation="MKRMLINATQQEELRVALVDGQKLYDLDIETPSREQKKSNVYKG
                     RITRIEPSLEAAFIDYGAERHGFLPFKEIARGYFQESVRGEGSRPSIKEALREGQEVV
                     VQVEKEERGNKGAALTTYISLAGRYLVLMPNNPRAGGVSRRIEGEDRQEIREAMAALN
                     VPDGMGLIVRTAGVGRSTEELQWDLDYLLNLWRAIEDASQQKKAPFLIYQESNIIIRA
                     LRDLLRADIGEILVDHPQTHEQAREFTQQVMPGSTAKLKLYQDATPLFTRFQIESQIE
                     TAYQREVSLPSGGAIVIDYTEALVSIDINSARATKGADIEETALNTNLEAADEIARQL
                     RLRDLGGLVVIDFIDMTVPRNQREVENRLKDALKMDRARVQVGRISRFGLLEMSRQRL
                     RPSLDEATHVLCPRCKGHGTIRGTESLALSVLRLIEEEAMKDKTGKVIAQLPVPVATF
                     LLNEKRAALAMLEERLGVRIALVPNETLDTPHFSIERVRVDDLAAGRASGPSYTLAVR
                     QEEPDLSLAAQQVAKLAAEEAAVRAIAPATPAPVTPAPRRAEAAPAASPAELAAPAGI
                     EAPGEDEPGFFRWLWRNLFGGPGAELPAALAPAAARSTSAPRAAAAEEPRRERPERAE
                     RPERPERERRDRRDRQRPERNDRRPAADTPVQPEPAVNPPLAEAPLPDALPAPAPAPV
                     PEPENIPAAPVMRLPEPPDADEDVRAPVEAGDGAGGDDAGDDARGGRRGRRGGRRRRR
                     GNEREAGTGAELEAGEGGDAAAEAGAAVEAGGGESFASTETIETTEAVESVEAA"
BASE COUNT          704 a         1641 c         1702 g          615 t
ORIGIN      
        1 ccaggtggca cggaagaaca gccgccagcg ccgctcccgc cggcgttcgc gcagcagctc
       61 gcgcgcgcgg tcggcgagca ctgcgagcgc gctggcctcg accggcgccg gcgcggcggt
      121 gcccggggcc ggcggcggcg atgcgggttc cttccacggg tccggcggcg gctggtcgct
      181 catgcgggtg gctccataca ggggttcagg cggccgccgc cggcagcgag gcgagcagcg
      241 ccggcagatc ggcgatgtcg tcgaggcagg cgagcggcgc atgccgcagc agcagttcgg
      301 gggcgtggca gccccaggtc gcgccgatgc ccgccatgcc ggcggcgcgc gccatcgcca
      361 gatcgaattc ggtgtcgccg atcatcagcg cgcgcgcggc cggcacgccg gtctcggcga
      421 gcagttcgtg cagcatgcgc ggatcgggtt tcgacgcggt ttcatcggcg cagcggctcg
      481 cctcgaacag ccgcccgagg ccggtgtcgc gataggcgcg ttcgaggccg gcgcggctct
      541 tgccggtggc gaccgccagc cggtagccgt cggcgcaaag cgcttcgagc accccggccg
      601 cgccggcgaa cagctgcatg ccggccggat cgcggctcag gaattcgtgc ttgtaggcct
      661 cggcgagccg caggtgctcg tcgtcgccgg cgagcggata cagcatcgcg caggcctcga
      721 tcaggccgag gccgatcacg tgccggtagg cggcatcggc gagggccggc aggccgaggc
      781 tcaggctcgc ggcgcgcagg caggcgacga tccggtactc ggaatccatc agcgtgccgt
      841 cccagtcgaa cacgagcagg tcgaaggcgc gatcgggcat cgtcgggcga ctccgggcag
      901 ggcagggggg ctcagccgcg cgcggcgagg gtgtcgagca cgcgcgcgag ttccgggcag
      961 agcggcgctt ccagcgtcag cgggcgcccg ccgagcggca ggctcagcag ctgcgcgtgc
     1021 aggaacaggc gccgcaggcc gagggcggcc agcgtgcggt tgaaggccgg atcgccgtac
     1081 ttgtcgtcac cggcgagcgg atggccgagg tgcgcggcat gcacgcggat ctggtgcgtg
     1141 cggccggtct cgatcgtgac ctcgaccagc gtgcagtcgg cgtgccggcg cagcggccgg
     1201 aagcggctca gcgccggctt gccgtcgggc gcgacctcga ccatgcgctc gccgccgcgg
     1261 atcatgccct tgcgcagcgg cgcatcgacg acgtgctcgc cgccgcgcca ggcaccgcgc
     1321 accagcgcca gatagcgctt gtcggtgctg cgggtgttca gcgcatcgtg gatcgcgcgc
     1381 agggtttccg gcgtcttggc gagcaccagc acgccgctgg tgtcgcgatc gaggcggtgc
     1441 gcgagttcga ggaacacctc ggggcgcagc acgcgcaggc cctcgatgac gccggcgtcg
     1501 atgccgctgc cggcatgcac ggcaatgccc gagggcttgt cgagcaccag cagccgggca
     1561 tcctcgtaca ggatcgcggc ttcgagccgt tcgagcaggc cgcgcgcggg catggccggc
     1621 gcatcgtctt cggcggcgac gcggatcggc ggcagccgca cgctgtcacc ggccgcgacg
     1681 cggtgctcgg ggcgcgcgcg acccttgttc acgcgcacct cgccggttcg caggatgcgg
     1741 tagacgaggc tcttcggaac gcccttgagc gtacggatca ggaagttgtc gatgcgctgg
     1801 ccgtcgcatt cggcgggaac ctcgacgaag cgcacggccg ggggcggaga agactggctc
     1861 atggccgcat gataaccgca gaagccccgt gcaggctcat ttgtgcggcg gcgcgaggca
     1921 ctgttatatt ccggatcgtt gtcgccggcc ggcagccgat ttcatccatc ggtccggtgg
     1981 tcgcgaaaca ctcgataagt tttcggtaca cgcccggaat cctgcgcttc gctgcatgcc
     2041 tgaaggcgca accgggcggg tatccaaggg gtttttccat cgctcgaccc tgccttaatt
     2101 tccagagcga tgccattcgc gctcccgcgc ccactgccgc gacggcgcgg cgacgaggtc
     2161 ccgggcagca ccggccgtga gtaccccggc tggccggcgc ccggcctgtc tgccgtgcct
     2221 ttccccgtag tagcggcgac aacgccgtgg gcttccggtc cgagcgccgg agacgtcttg
     2281 catgaagaga atgctgatca acgccactca gcaggaagag ttgcgcgttg cgctggtcga
     2341 tggccagaag ctctacgacc tcgacatcga aaccccctcc cgcgagcaga agaagtccaa
     2401 cgtctacaag gggcggatca cgcgcatcga gccttcgctc gaagccgcct tcatcgacta
     2461 cggcgccgag cgccacggct tcctgccgtt caaggagatc gcccgcggct acttccagga
     2521 gtcggtgcgc ggcgaaggct cccggccgtc gatcaaggag gcgctgcgcg agggccagga
     2581 ggtcgtcgtc caggtcgaga aggaggagcg cggcaacaag ggtgcggcgc tgaccaccta
     2641 catctcgctc gccgggcgct acctggtgct gatgccgaac aacccgcgtg ccggcggcgt
     2701 gtcgcggcgc atcgaggggg aggatcgcca ggagatccgc gaggcgatgg cggcgctgaa
     2761 cgtgcccgac ggcatgggcc tgatcgtgcg caccgccggc gtcggccgct cgaccgagga
     2821 gctgcagtgg gacctcgact acctgctgaa cctctggcgc gcgatcgagg atgcctcgca
     2881 gcagaagaag gcgccgttcc tgatctatca ggagagcaac atcatcatcc gcgcgctgcg
     2941 tgacctgctg cgcgccgaca tcggcgagat cctcgtcgat cacccgcaga cgcacgagca
     3001 ggcgcgcgag ttcacgcagc aggtgatgcc gggcagcacc gcgaagctga agctgtacca
     3061 ggacgcgacg ccgctgttca cgcgcttcca gatcgagtca cagatcgaga ctgcctacca
     3121 gcgcgaggtc agcctgccct cgggcggcgc gatcgtcatc gactacaccg aggcgctggt
     3181 gtcgatcgac atcaactcgg cacgcgccac caagggcgcc gacatcgagg aaaccgcgct
     3241 caacaccaac ctcgaagccg ccgacgagat cgcccgccag ctgcgcctgc gcgacctcgg
     3301 cggcctcgtg gtcatcgact tcatcgacat gaccgtgccg cgcaaccagc gcgaggtcga
     3361 gaaccgcctg aaggacgcgc tgaagatgga tcgcgcgcgc gtgcaggtcg gacgcatctc
     3421 gcgcttcggg ctgctggaga tgtcgcgcca gcgcctgcgc ccgtcgctcg acgaggccac
     3481 gcacgtgctc tgcccgcgct gcaagggcca cggcacgatc cgcggcaccg agtcgctggc
     3541 gctgtcggtg ctgcggctga tcgaggaaga ggcgatgaag gacaagaccg gcaaggtcat
     3601 cgcgcaactg ccggtgccgg tcgccacctt cctgctcaac gagaagcgcg ccgcgctcgc
     3661 catgctcgag gagcggctcg gcgtgcgcat cgcgctggtg ccgaacgaga ccctggacac
     3721 gccgcacttc tcgatcgagc gcgtgcgcgt cgatgacctc gccgccggcc gcgccagtgg
     3781 tccgagctac acgctcgccg tgcgtcagga ggagcccgac ctgtcgctgg ccgcgcagca
     3841 ggtcgcaaag ctcgccgccg aggaagccgc ggtgcgcgcg atcgccccgg ccacgccggc
     3901 gccggtcacg ccggccccgc gccgcgccga agcggcaccg gccgcatccc cggccgaact
     3961 cgccgcccct gccggcatcg aggcacccgg cgaggacgag ccgggcttct tccgctggct
     4021 gtggcgcaac ctgttcggcg gccccggagc cgagctgccg gccgcgctcg ccccggcggc
     4081 agcccgcagc accagtgccc cgcgtgctgc cgcagccgag gagccgcgcc gcgagcgtcc
     4141 ggaacgcgcg gagcgtccgg agcgtccgga gcgtgagcgg cgcgatcggc gcgaccgtca
     4201 gcgcccggag cgcaacgatc gccgtccggc cgccgatacg ccggtgcagc ccgagccggc
     4261 cgtgaacccg ccgctcgccg aagcgccgct gccggacgcg ctgccggcgc cggctcccgc
     4321 gccggttccg gagcccgaaa acatcccggc cgcgccggtc atgcgcctgc ccgaaccgcc
     4381 ggatgccgat gaagacgtgc gcgctccggt ggaagccggg gatggggccg gtggcgacga
     4441 tgccggtgat gatgcgcgcg gcggtcgtcg cggccgtcgc ggtggccgtc gtcgccgtcg
     4501 cggcaacgag cgcgaagccg gaaccggcgc cgagctggaa gccggcgagg gcggtgacgc
     4561 ggcggccgaa gcgggcgccg cggtcgaggc cggtggcggt gaatccttcg cgtccaccga
     4621 aaccatcgaa accaccgaag ctgtcgagtc cgtcgaagcc gc
//
LOCUS       DAOQIE010000296         4633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10786_length_4633_cov_7.006990, whole genome shotgun sequence.
ACCESSION   DAOQIE010000296 DAOQIE010000000
VERSION     DAOQIE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10786_length_4633_cov_7.006990"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2878)
                     /locus_tag="PK144_15275"
     CDS             complement(<1..2878)
                     /locus_tag="PK144_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF67137.1"
                     /translation="MPTATAQALVTASADGLRARVEGAAGAGADWTASREEAARRLRD
                     WAERHDRLAGLDDADSELLEIGREMARWLDDSGWLSGWLRRPGARRLILQSPDVEHDL
                     GRLLHDAPWELLTAPGAESGHLAGDPLRPLELARRLGAPEDPQQPVHGDLCLLFMAAA
                     PQGETVLDFEAEESSILEATRSLHQLLLTVEESGSLAGLQQRLCASEGVAPDIVHLSC
                     HGGVDDGLPVLYLEDERGQRQAVPAARLAEVLSASPELRLVVLSACGTAAQTSDGEGR
                     RSTLVTDLICSGVDQVLGWDGSVADRDATQFAAEFYKALTARQDVVAAAARARLRLHT
                     EHAQEARCGRHWHMARVYLGPRGGQTLLAQGGQRRALRSAVGEEHFLDKARAAVPVAT
                     RSHFVGRRRAIQQVLGAFEDGRAALVHGMGNLGKSSLAARIAHDRLPQLACVVVFGRY
                     DALEVWDRLLDACTPAERPPFRDRWRSRIAADERQLADALDDLLAGPLAARPVLLIID
                     DLERVLEEPRPDQPSVRVQPERRPMLAAVLRAFGRQQRSKLLLTSRYTFSLPGSGGRD
                     LAADLVLVPLRAMDESDRRKQQRALLRDLPERQVARLDLALLQRAGELADGNPGLQAL
                     LSQPVLAGEAQAAGQAFAVIEHYLQHGVPPAAIQHELASGRAADERNAVLAFFRRMAF
                     DTYRNALSSQQLRALQAGCLFASGLPLPLPALQAAAGAAGVRDAAAAVQRLLGLGLLD
                     DHGPLADIAHAAVNPLARPLVDAIADDDARQWAAAALPALADAWIDGDTFRWPVAFAA
                     EAWRLIERAGSPAPARLIDAATAVLARHRHEQGDRAQAILDQLVRPARQALAAQGHAP
                     SRDFWSAVHDVANRAGDTGLRMASVAGLKNRATSPFERGSALLREAWAAQSSGDPDGA
                     DGCFAEAAELFRSAGADREVAIARGGQADILQARGQLDEALRIRL"
     gene            complement(2972..3835)
                     /locus_tag="PK144_15280"
     CDS             complement(2972..3835)
                     /locus_tag="PK144_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67138.1"
                     /translation="MPAISRRLAGMARDHVQEASVMSAHAYLSQLQLAPVAVRATISD
                     STTHALRSGAGSAEAAADTAGIPLTDVRAAVDVGSLVSFVDGLDAQSKDDVLDSLQLA
                     QRAANAAADRFADVRNWYHQYLDVLGQIGWVTGQFAFLEREQSEGDLRMDQAALSVLM
                     AIATQNQLAILKEAVSALGSLASSDGTISLFAQMSALEGLGNFQMGAAQRAGNGVVEL
                     AMGAYHFRAVDSTDRIVFFKRSTRALKLWTAAQRMTLNTASYAENRAQIRQMLGEERQ
                     RAIAAIRLKPL"
     gene            4107..4406
                     /locus_tag="PK144_15285"
     CDS             4107..4406
                     /locus_tag="PK144_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNF67139.1"
                     /translation="MKRVNRHYPPEFRKASVQQVVDGGRSDPAVARSLEMSAKTLANW
                     VARARRGEAPTQGSMRGPVSELEPEVTRLRQEVAKLRMEKGILKKAAAYFAKESM"
     gene            4403..>4633
                     /locus_tag="PK144_15290"
     CDS             4403..>4633
                     /locus_tag="PK144_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNF67140.1"
                     /translation="MKYSWIEAHIGSYPVRPMCELLGVSASGFYTARSRAPAARTAEQ
                     VRIVAEIRRAQTRHQGRYGRRRMTAEVGAVLGR"
BASE COUNT          653 a         1571 c         1708 g          701 t
ORIGIN      
        1 ccagccggat gcgcagcgcc tcgtcgagct gcccgcgcgc ctgcaggatg tcggcctgcc
       61 ccccgcgcgc aatcgcgacc tcccggtctg caccggccga acggaaaagc tccgccgcct
      121 ccgcaaagca gccatcggcg ccgtcgggat caccgctgga ctgggcggcc catgcttccc
      181 gcagcagcgc cgagccacgc tcgaagggcg aggtggcgcg gttcttgagc cccgcgacgc
      241 tcgccatgcg caagccggtg tcgccagcgc ggttcgccac gtcatggacg gcagaccaga
      301 agtcgcggga cggggcatgg ccttgcgcag cgagcgcctg gcgcgccggc cggaccagtt
      361 gatcgaggat ggcctgggcg cggtcgccct gctcgtggcg atggcgggcg agcacggcgg
      421 tggcggcgtc gatcagccgg gccggcgccg gcgaccctgc ccgctcgatc aggcgccagg
      481 cctcggcggc aaaggcgacc ggccagcgga aggtgtcgcc gtcgatccac gcgtctgcga
      541 gcgccggcag cgcggcggcc gcccattgcc gggcgtcgtc gtccgcgatg gcatcgacca
      601 gcgggcgggc gagcgggttg acggcggcgt gcgcgatgtc ggccagtggc ccgtggtcgt
      661 cgagcaggcc gaggccgagc aggcgctgca cggccgcggc ggcatcgcgg acgccagcgg
      721 caccggcagc ggcttgcagc gctggcagtg gcagcggcag gccgctggcg aacaggcagc
      781 cggcctgcag cgcgcgcagc tgctggctgc tcagcgcatt tcggtaggtg tcgaaggcca
      841 tgcgccggaa gaaggcgagc acggcgtttc gctcgtcggc cgcgcggccg gatgccagtt
      901 cgtgctggat cgccgccggc ggcacgccgt gctgcagata gtgctcgatg acggcaaacg
      961 cctgcccggc ggcctgcgcc tcgccggcga gcaccggctg gctgagcagc gcctgcagtc
     1021 cggggttgcc gtcggccagc tcgccggcgc gctgcagcag ggcgaggtcg agacgagcga
     1081 cctgccgctc cggcaggtcg cgcagcaacg cacgctgctg cttgcgccgg tcgctctcgt
     1141 ccatggcgcg aagcggcacg agcaccaggt cggcggcgag gtcgcgccca cccgagccgg
     1201 gcaggctgaa ggtgtagcgg ctggtcagca gcagcttgct gcgttgctgc cggccgaagg
     1261 ctctgagcac cgcagcgagc atcggccgcc gttcgggctg gacccggacg ctcggctggt
     1321 cgggccgtgg ttcttcgagc acgcgctcga gatcgtcgat gatcagcagc accggccgcg
     1381 cggcgagcgg gccggcgagg aggtcgtcga gcgcgtcggc cagttgtcgc tcgtcggcgg
     1441 cgatccggct gcgccagcga tccctgaatg gcggccgctc ggcgggcgtg caggcgtcca
     1501 gcaggcggtc ccagacttcc agcgcgtcgt agcggccgaa caccaccaca caggcgagct
     1561 gcggcaggcg gtcgtgtgcg atgcgcgcgg ccaggctcga cttgccgagg ttgcccatgc
     1621 cgtgcaccag cgccgcccgg ccgtcctcga aggcgccgag cacctgctgg atcgcgcgcc
     1681 gccggccgac gaagtggctg cgcgtcgcca ccggcaccgc ggcgcgtgcc ttgtcgagaa
     1741 aatgttcttc gccaacggcg ctgcgcagcg cccgccgctg cccgccctgg gcgagcagcg
     1801 tttgcccgcc acgcggcccg aggtagaccc ttgccatgtg ccagtgccgg ccgcagcgtg
     1861 cttcctgtgc atgctcggtg tgcagccgca gccttgccct ggccgcggca gcgacgacat
     1921 cctgccgcgc ggtcagcgcc ttgtagaact cggcggcgaa ctgcgtcgcg tcgcggtcgg
     1981 ccaccgaccc gtcccagccg agcacctgat cgacgccgct gcagatcagg tcggtcacca
     2041 gcgtgctgcg tcgcccttcg ccgtcgctgg tctgcgccgc cgtgccacag gcggagagca
     2101 cgaccagccg cagttccggg ctggcggaca agacctcggc gagtcttgcc gcgggcaccg
     2161 cttgccgctg gccgcgttcg tcttcgaggt acagcacggg caagccgtcg tccacgccgc
     2221 cgtggcagct gaggtggacg atgtccggcg cgacgccttc gctggcgcac aggcgctgct
     2281 gcagaccggc gagcgagccg ctttcctcga cggtcagcag cagttgatgc aggctcctgg
     2341 tagcctcgag gatgctggat tcctcggctt cgaagtcgag caccgtttca ccctgcggcg
     2401 cggccgccat gaacagcagg cagagatcgc cgtgcacggg ctgctgcggg tcttccgggg
     2461 cgccgaggcg gcgtgccagc tcgagcggcc gcagcgggtc gcccgccagg tggccggact
     2521 cggcacccgg cgcggtcagc agctcccacg gggcgtcgtg cagcaggcgt cccaggtcgt
     2581 gctcgacatc gggcgattgc aggatcagcc ggcgcgcgcc gggacggcgc agccagccgc
     2641 tcagccagcc ggaatcgtcg agccagcgcg ccatttcgcg gccgatctcg agcagctcgc
     2701 tatccgcgtc gtccaggccg gccagccgat catggcgttc ggcccagtcg cgcagccgtc
     2761 gcgcagcctc ctcgcggctg gcggtccagt cggcgcccgc gccggccgcg ccttccacgc
     2821 gggcacgcaa gccatcagcg ctggcggtga cgagggcttg cgcggttgca gtcggcatgg
     2881 tggtgggttc ctttcggttt gatcgatgtc tgttgctgct ctctgtcgga aacacgcggc
     2941 gcgggggcaa ccgatgcccg tgccgggccg atcagagcgg cttgagccgg atcgccgcga
     3001 tcgcgcgctg gcgttcctcg ccgagcatct gccggatttg cgcccggttt tccgcatagc
     3061 tggcggtgtt cagcgtcatc cgctgggcag ccgtccacag cttcagcgcc cgcgtgctgc
     3121 gcttgaagaa gacgatccgg tcggtgctgt ccacggcccg gaagtggtag gcgcccatcg
     3181 ccagctcgac cacgccgttg ccggcacgct gcgccgcgcc catctggaag ttgcccagcc
     3241 cctcgagggc gctcatctgc gcaaacaggc tgatggtgcc gtcgctgctg gccagcgagc
     3301 cgagcgcgga taccgcctcc ttcaggatgg ccagctggtt ctgcgtagcg atcgccatca
     3361 ggacggacag cgcggcctgg tccatgcgca ggtcgccttc gctctgctca cgttccagga
     3421 aggcgaactg cccggtcacc cagccgatct ggccgagcac gtcgaggtac tggtggtacc
     3481 agttgcgcac atcggcaaag cggtcggcgg cagcgtttgc cgcacgctgg gcgagctgca
     3541 gggaatcgag cacgtcgtcc ttgctctgcg cgtcgagtcc gtcgacgaag ctcaccaggc
     3601 tgccgacatc gacggcggcg cgcacatcgg tgagcggtat cccggccgtg tctgctgcgg
     3661 cttcggcact gcccgcaccg gaacgcagcg catgcgtcgt cgaatcggag atcgtggcgc
     3721 gcacggcgac gggcgcgagt tgcaactgtg agagataggc atgtgccgac atgaccgagg
     3781 cctcctgaac gtgatcgcgg gccatgccgg cgaggcgacg gctgatggcc ggcaagcggc
     3841 cagagtccgc cgtcccgcgc aggattggca cgcctccctg ctgcgaatcc gatgccgtcc
     3901 attctagctg atggcacacg acacctggcg cgtattcgtg tttttcgacg cgttcggctc
     3961 gatccgatcc gccgccggtt cgaacctacg cgtcggcagc ctgttcatgg ccggaggcgg
     4021 tggagtcgga aaacgggcca tttctggcat ggccgtccag gccccgtctg cgcatcgggc
     4081 gcagcgtatc cttgtggagg ttggacatga agagagtgaa tcggcattac ccgccggagt
     4141 ttcggaaggc gtcggtgcaa caggtggttg atggcgggcg cagcgatccg gcggtggcgc
     4201 ggtcgttgga gatgtcggcg aagacgttgg cgaactgggt cgcgcgggcg cgtcgaggcg
     4261 aggcaccgac ccaagggtcg atgagagggc cggtgagcga actggaaccg gaggtgacgc
     4321 gattgcggca agaggttgcg aagctgcgga tggagaaagg gatcctaaaa aaagcggcgg
     4381 cgtacttcgc caaggagtcg atgtgaagta ctcgtggatc gaagcgcata tcggctccta
     4441 tccggtgcgg ccgatgtgcg agttgctagg ggtatcggcc agtggtttct acacggcgcg
     4501 gtctcgggct ccggcggctc gcacggccga acaggtgcgg atcgtggcgg agattcgtcg
     4561 tgctcagacg cggcatcagg gccgctatgg tcgtcggcga atgacggccg aggtgggtgc
     4621 cgttctgggc cga
//
LOCUS       DAOQIE010000297         4592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_10921_length_4592_cov_8.204320, whole genome shotgun sequence.
ACCESSION   DAOQIE010000297 DAOQIE010000000
VERSION     DAOQIE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10921_length_4592_cov_8.204320"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK144_15295"
     CDS             <1..387
                     /locus_tag="PK144_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF67141.1"
                     /translation="DKGGRAGSGTGGRVDAGLRTTLAGEELRVAHVLDGGPAQAAGLA
                     AGDVLVALDGLRVQPRGFERQLGRYAPGARVELLGFRRDELMRFPLELAAAPADTVWI
                     TPDPQADAEALQRRQAWLGETGVSPG"
     gene            485..1228
                     /gene="minC"
                     /locus_tag="PK144_15300"
     CDS             485..1228
                     /gene="minC"
                     /locus_tag="PK144_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNF67142.1"
                     /translation="MTQTPTAEAPFELKGSLFTLTVLHLRETDLGAIEQALLVKIAQA
                     PAFFANTPVVVDLDALADTAARPDFAGLARILRAHGLVPVGVRHGSAAQLAAAAAAGL
                     PMLPESRPALRREATTDAGGDAKPADPGPHNRVVVQPVRSGQQVYAADGDLTLLGPVS
                     AGAEVLADGSIHVYGPLRGRALAGVRGKLEARIFCLGLEAELVSVAGRYRILEKNGEG
                     WGKAVQIYLSGEHLIIEPLTVEAGAHRKS"
     gene            1240..2049
                     /gene="minD"
                     /locus_tag="PK144_15305"
     CDS             1240..2049
                     /gene="minD"
                     /locus_tag="PK144_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNF67143.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASFATGLALRGHKTVVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVINNEATLNQALIKDKRVESLYVLPASQTRDKDALHKEGV
                     ERVLEELKQSFDYIICDSPAGIERGAMMAMYFADEALVVTNPEVSSVRDSDRILGILA
                     SKSRRAEQAEEPVREHLVLTRYSPERVEKGDMLSVDDVMEILAIPLLGVIPESSAVLQ
                     SSNSGVPVVLDEQADAGIAYADLVARFLGEERSHRFLSVERKGFFKRLFGN"
     gene            2052..2366
                     /gene="minE"
                     /locus_tag="PK144_15310"
     CDS             2052..2366
                     /gene="minE"
                     /locus_tag="PK144_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNF67144.1"
                     /translation="MGFFDYFRSSRHKDNTAAVAKERLQIIVAHERGANRRHSPDYLP
                     ALKRELLEVVRRYVQVEQDQIKVHLDRDGDFEVLELNILLPDDESARVTVTTPSAPKP
                     AR"
     gene            2384..2794
                     /locus_tag="PK144_15315"
     CDS             2384..2794
                     /locus_tag="PK144_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019589205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNF67145.1"
                     /translation="MSGVADAGAGRVRLDKWLWAARFFKTRALATEAVSGGHVHVGGE
                     RVKPARSLHPGDRLDITRGHERFEVIVRALSEQRGPASVAQTLYEETAASRSRREHEA
                     EQHRLQALANPRPDARPDKQARRRILRFVREGGG"
     assembly_gap    3023..3122
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3123..4277
                     /locus_tag="PK144_15320"
     CDS             <3123..4277
                     /locus_tag="PK144_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNF67146.1"
                     /translation="LDNAPDRPPAPPAGGALPRQPHPPGLPVAAVPLRMRVQFHGEGG
                     EFFRIWIVNLLLTVLTLGIYSAWAKVRTRRYFYGCTSLAGSTFEYLAEPVAILKGRAV
                     LAVALLLLYASLSMMPLFGAALLVGALPFVPALIVSSLRFNYRVTRWRNVAFRFTGSY
                     GGAAVAFLLWPLLGVVSFGLLWPKAIQAQRSYLLNHVHYGNAAFSADIPLWPVYGCSI
                     VASVLLGIVTAVATVLSGLMLARAGLRPQSMADLDHWQLFALVLPTLAYLFGLTLSAA
                     YYRAALRNIVLADVAIGDGHGLRSHVSVLDYIAFATGNLLLTVVTLGLARPWVRVRTA
                     RYFATTTTIRFAVPAGAAMAGIAALTEAAGRRGSTLGDAAGDAFDWDLSL"
     gene            4274..>4592
                     /locus_tag="PK144_15325"
     CDS             4274..>4592
                     /locus_tag="PK144_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67147.1"
                     /translation="MSEAGSLRIAGCHYPAGSAAGQAAELHVSADGALRLEPGGRALG
                     HWRGLRVPERLGRAPRVLGLGDGGGFETGDNAAVDACERRFRGRVAGGRLHALEGRWT
                     IAWL"
BASE COUNT          610 a         1603 c         1599 g          680 t
ORIGIN      
        1 gacaagggcg gccgggccgg cagcggcacg ggcgggcgcg tcgatgccgg cttgcgcacg
       61 acgctcgccg gcgaggagct gcgcgtcgcg cacgtgctcg acggcgggcc ggcgcaggca
      121 gccgggctcg cggccgggga cgtgctggtc gccctcgatg gtctgcgcgt gcagccgcgc
      181 ggcttcgagc gccagctcgg gcgctacgcg ccgggggcgc gcgtcgagct gctggggttc
      241 cggcgcgacg agctgatgcg cttcccgctc gaactcgccg ccgccccggc cgacaccgtc
      301 tggatcacgc ccgacccgca ggcggacgcc gaagccctgc agcgccgtca ggcctggctc
      361 ggtgagaccg gcgtgtcgcc ggggtgagct tgaacggtcg ggggcgagtg tttagagtca
      421 ccgccgtctg cggcccgcaa cagatcacaa caattccgga caggggacgt acgctcccga
      481 cgccatgacc cagacgccga ccgccgaggc gcccttcgag ctcaagggca gcctgttcac
      541 gctgactgtg ctgcatctgc gcgagaccga tctcggcgcg atcgagcagg cgctgctcgt
      601 caagatcgcc caggccccgg cgttcttcgc caacaccccg gtcgtcgtcg atctcgacgc
      661 gctcgccgat acggcggcga ggccggattt cgccgggctg gcgcggatcc tgcgcgccca
      721 cgggctggtg ccggtcggcg tgcgtcacgg cagtgccgcg cagctcgccg ccgccgccgc
      781 cgccgggctg ccgatgctgc cggaaagccg tccggcgctg cggcgcgaag caacgaccga
      841 tgccggcggc gatgcgaaac ccgcagaccc cggcccgcac aaccgcgtcg tcgtgcagcc
      901 ggtgcgctcc ggccagcagg tctacgcggc cgacggcgac ctgacgctgc tcggtccggt
      961 cagtgccggt gccgaggtgc tcgccgatgg cagcatccat gtctacggac cgctgcgcgg
     1021 ccgggcgctg gccggcgtgc gcggcaagct tgaagcccgc atcttctgcc tcggcctcga
     1081 agccgagctg gtgtcggtcg ccggccgata ccgcatcctc gaaaagaacg gcgaaggctg
     1141 gggcaaggcc gtgcagatct acctgtccgg cgaacacctg atcatcgagc cgctcaccgt
     1201 cgaagccggc gcgcaccgca agtcctagga gacacaccct tggccaagat catcgtcgtc
     1261 acctccggga aggggggcgt gggcaagacc acgaccagtg cgagctttgc caccgggctc
     1321 gcgctgcgcg ggcacaagac cgtcgtcatc gacttcgacg tgggtctgcg caacctcgac
     1381 ctgatcatgg gctgcgagcg gcgcgtcgtg tacgacttcg tcaacgtcat caacaacgag
     1441 gcgacgctga accaggcgct gatcaaggac aagcgcgtcg agagcctgta cgtgctgccg
     1501 gcctcgcaga cgcgcgacaa ggatgcgctg cacaaggaag gcgtcgagcg cgtgctcgag
     1561 gagctcaagc agagcttcga ctacatcatc tgcgactcgc cggccggcat cgagcgcggc
     1621 gcgatgatgg cgatgtactt cgccgacgag gcgctggtcg tcaccaatcc cgaggtgtcg
     1681 tcggtgcgcg attccgaccg catcctcggc atcctcgcca gcaagtcgcg ccgcgccgag
     1741 caggccgagg agccggtgcg cgagcatctg gtgctgacgc gctactcgcc cgagcgcgtc
     1801 gagaagggcg acatgctgtc ggtcgacgat gtcatggaga tcctcgcgat cccgctgctc
     1861 ggcgtgatcc cggagtcctc ggcggtgctg cagtcgtcga actccggcgt gccggtcgtg
     1921 ctcgacgagc aggccgatgc cggcatcgcc tacgctgacc tggttgcccg tttcctcggc
     1981 gaggagcgct cgcaccgctt cctgagcgtc gagcggaagg gcttcttcaa gcgcctgttc
     2041 ggtaactgaa catgggcttc ttcgactatt tccgttcctc ccggcacaag gacaacaccg
     2101 ccgcggtcgc caaggagcgc ctgcagatca tcgtcgcgca cgagcgcggc gcgaaccgcc
     2161 ggcacagccc cgactacctg ccggcgctca agcgcgagct gctggaggtc gtgcgccgct
     2221 acgtgcaggt cgagcaggac cagatcaagg tgcatctgga ccgcgacggc gacttcgagg
     2281 tgctcgaact gaacatcctg ctgcccgatg acgaatcggc gcgggtgacg gtgacgacgc
     2341 cgtcggcgcc gaaaccggcg cgctgacgca ggaaaccgca gcggtgagcg gggtggccga
     2401 tgccggggcc gggcgtgtcc gcctcgacaa gtggctgtgg gccgcgcgct tcttcaagac
     2461 ccgggcactc gcgaccgagg cggtgagcgg cggacacgtg catgtcggcg gcgagcgcgt
     2521 gaagccggcg cgcagcctgc atccgggcga ccggctcgac atcacgcgcg ggcacgagcg
     2581 cttcgaggtc atcgtgcgcg cgctgtcgga gcagcgcggg ccggccagcg tcgcgcagac
     2641 gctgtacgag gaaaccgccg cctcgcgcag ccgccgcgag cacgaggccg aacagcaccg
     2701 gctgcaggcc ctcgcgaatc cccgcccgga tgcccgcccc gacaagcagg cgcgccggcg
     2761 catcctgcgc ttcgtgcgcg agggcggggg ctgaaagccg caggcgctgc ggttggccgg
     2821 acgcttcacc tctgtcatgc tgcggcgcag cgaccgcagg atccgccggg ttctctgcgc
     2881 ccggcctgaa caggagggat gccgatgctg ccgcccgctt catgccgcgc cccgtacgat
     2941 gatttcgaag ggctgagtct ggtgccgctc gacaacgcgc cggatcgccc gccggctccg
     3001 ccggcgggcg gtgcgctgcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnctcgacaa cgcgccggat cgcccgccgg ctccgccggc gggcggtgcg ctgccgcgcc
     3181 agccgcatcc gcccggactg ccggtcgccg ccgtgccgct gcgcatgcgc gtgcagtttc
     3241 atggcgaggg cggcgagttc ttccgcatct ggatcgtcaa cctgctgctg acggtgctga
     3301 cgctcggcat ctactcggcc tgggccaagg tgcgcacgcg ccgctacttc tacggctgca
     3361 cctcgctcgc cggttcgacc ttcgagtacc tggccgagcc ggtggcgatc ctgaaggggc
     3421 gggcggtgct ggcggtcgcc ctgctgctgc tgtatgcgtc gctgtcgatg atgccgctgt
     3481 tcggcgccgc cctgctggtc ggcgcgctgc cgttcgtgcc ggcgctgatc gtctccagcc
     3541 tgcgcttcaa ctaccgggtg acgcgctggc gcaacgtcgc cttccgcttc accggcagct
     3601 acggcggcgc ggccgtggcc ttcctgctgt ggccgctgct cggcgtcgtg agtttcggcc
     3661 tgctgtggcc gaaggccatc caggcgcagc gcagctatct gctgaatcac gtgcactacg
     3721 gcaacgctgc gttttcggcc gacatcccgc tgtggccggt ttacggctgc tcgatcgtcg
     3781 ccagcgtgct gctcggcatc gtcaccgcgg tggcgacggt gctgagcggg ctgatgctgg
     3841 cgcgcgccgg cctgcggccg cagtccatgg cggatctgga ccactggcag ctttttgcac
     3901 tggtgctgcc gacgctcgcc tacctgttcg ggctgacgct gtccgcggcc tattaccgcg
     3961 cggcactgcg caacatcgtg ctcgccgacg tggcgatcgg cgacggccac ggcctgcgca
     4021 gccatgtgtc ggtgctcgac tacatcgcct tcgcgaccgg caacctgctg ctgaccgtcg
     4081 tcacgctcgg gctggcgcgc ccgtgggtgc gcgtgcgcac cgcacgctat ttcgcgacca
     4141 cgaccacgat ccgtttcgcc gtgccggccg gcgcggccat ggccggcatc gccgcgctca
     4201 ccgaggcggc cggtcgtcgc ggctcgacgc tcggcgatgc cgccggcgat gccttcgact
     4261 gggatctgtc gctgtgagtg aagccgggag cctgcgcatc gccggctgcc actacccggc
     4321 cggcagcgcc gccggccagg ccgccgaact gcacgtcagc gccgatggcg cgctgcggct
     4381 cgaacccggc ggtcgtgcgc tcggtcactg gcgcggcctg cgcgtgcccg aacggctcgg
     4441 gcgcgcaccg cgcgtgctcg ggctcgggga cggcggcggc ttcgagaccg gcgacaatgc
     4501 cgccgtcgat gcctgcgaac ggcgcttccg cggccgggtc gccggtggcc ggctgcatgc
     4561 gctcgaaggc cgctggacga tcgcgtggct cg
//
LOCUS       DAOQIE010000298         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11001_length_4571_cov_6.160319, whole genome shotgun sequence.
ACCESSION   DAOQIE010000298 DAOQIE010000000
VERSION     DAOQIE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11001_length_4571_cov_6.160319"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK144_15330"
     CDS             <1..191
                     /locus_tag="PK144_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67148.1"
                     /translation="PLARRLRGRRGLLLRVALLAADQRIAALRSAAPLPLADARQTLT
                     LLALAVWHGIRPERKASS"
     gene            245..1096
                     /locus_tag="PK144_15335"
     CDS             245..1096
                     /locus_tag="PK144_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNF67149.1"
                     /translation="MHPDLYCADKAAPPGSPAHYCLLGSDAGTRATLGALWALERELA
                     GIVPECREPAVARAKFDWWRAELRTLEGGTPQHPVTQALAPRLRARGLDAARLLECLE
                     GHALVLDYDAWPNHAVLVQHALTPTGATPARLAVELLGAAELPGAAAGAHELGLAHAQ
                     FERLREVRADALRGHCTVPDDELAAHGLTHADLLKPQTSAATQALFAAQIVRIRATQA
                     RGLERLSPAARRALTPLVVRAALEAATLTEIEADGLALLERRVELTPLRLLWQAWRAR
                     RRARRGG"
     gene            1197..1952
                     /locus_tag="PK144_15340"
     CDS             1197..1952
                     /locus_tag="PK144_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HNF67150.1"
                     /translation="MNYDPQYAPAPDLLRERVILITGAGDGIGRAVARACAAHGATVI
                     LSGRTKKKLEAVYDEIESAGHPQPVIHPLNLESLTPTDVDTIAEAIDQNFGRLDGLLH
                     NAGWVGSLTPIANYEPELWLRVVQLNLSAPFLLTQALLGLLNRAPAASVVFTTDAVAQ
                     RAQAYYGAYAAAKGGIEVLARMLASELEANTRIRVNTIDPGEVRTNLRIRTFPGITPE
                     NWPRPEALVNAWLWLLGPDSDGVRGQLVQAQPR"
     gene            complement(1996..4161)
                     /locus_tag="PK144_15345"
     CDS             complement(1996..4161)
                     /locus_tag="PK144_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF67151.1"
                     /translation="MPLPRIPAAAARFVAGLLGLWLILSAGLAGAGVMTRTTLGQIYP
                     PPYSVGEQARELPVWPLSRMNGPYPELEGWLFESIDFAPIPGFSGTPVNLLVEIAPDG
                     QFRDVRVISQHEPVFLDGLGPEPLAAFVRQYVGLGLNRNIKVTSSTNGGVRLDSANAY
                     IDGVSKATASVRIVNDSIVNAALAVARAKLGIGGGSPARAGTIRSEVLDAGLDWAGLK
                     DKGLVACRQYSNREVERAFAGTVGEGQDPEALAAPEALYLEFCATLLNVPGVHAALLG
                     DDAWARIARTLEPGDLVLLAFSRGRYGFAGPDFVRNAIPDRLGLAQGGLPLGLRDMDL
                     DERVRVAGLPDFDTVKFFRVLAQAGLDPARPVELSVRTTRAHGLLYPERVSRDLGLEL
                     ALPARYVERAPQAPQPLSGWRAVWAERAGELLILLAGFVLLTLLLARPAWLTHDATRL
                     RRVRTLWLVFTLGFIGWYAQGQLSIVNLTAVIQALRAGRGLDFLLNDPMTVLVWAYTA
                     LALVVWGRGTFCGWLCPFGALQELAALGARALGLQPRRVPEALDARLMPVKYAVLALI
                     LAAAWFAPALSDRLVEIEPFKTAITLGFVRSLPFVAWALALIALNLVFYKAFCRWLCP
                     LGAALALLGRVRVFAWLPRRAECGSPCQLCRRSCEYGAIRRDGAIRYADCFQCLDCVA
                     IYRDRRRCAPLLLADKRAARGQAPVRFVPRAGGLEAGPS"
BASE COUNT          654 a         1780 c         1619 g          518 t
ORIGIN      
        1 cgccgcttgc ccgccggctg cgcggtcgcc gcggcctgct gctgcgcgtc gcgctgctcg
       61 cggccgacca gcgcattgcg gccctgcgca gcgccgctcc gctgccgctc gccgatgcgc
      121 gccagaccct gacgctgctc gccctcgccg tctggcacgg catccgcccc gaacgcaagg
      181 cgagcagctg accgccgccg ccgaccgccc cgacatcacg ccacaccaca ccccagcaca
      241 ccgcatgcat cccgatctct actgcgccga caaggccgct ccccccggct cgccggcgca
      301 ttactgcctg ctcggcagcg atgccggaac ccgcgcgacg ctgggcgcgc tctgggcgct
      361 ggagcgcgaa ctcgccggca tcgtgcccga gtgccgcgag ccggcggtcg cgcgcgccaa
      421 gttcgactgg tggcgcgccg agctgcgcac gctcgaaggc ggcacgccgc agcatccggt
      481 cacgcaggcg ctggctccgc gcctgcgcgc gcgcggcctc gacgccgccc gcctgctcga
      541 atgcctggaa ggccatgcgc tggtgctcga ttacgacgcc tggccgaacc acgccgtgct
      601 cgtgcagcac gcgctgaccc cgaccggcgc cacgccggcg cggcttgcgg tcgagctgct
      661 cggggcggca gagctgccgg gggcggcggc cggcgcgcac gaactcgggc tcgcacacgc
      721 gcagttcgag cgcctgcgcg aggtgcgcgc cgatgcgctg cgcggtcact gcacggtgcc
      781 ggacgacgaa ctcgccgcac acggcctgac gcatgcggat ctgctgaagc cgcagacgag
      841 cgccgccacg caggcgctgt tcgccgcgca gatcgtccgc atccgcgcca cgcaggcccg
      901 cgggctggaa cgcctgtcgc cggccgcgcg ccgggcgctg acgccgctcg tcgtgcgcgc
      961 cgcgctcgaa gccgcgacgc tcaccgagat cgaggccgac ggtctggcgc tgctcgaacg
     1021 gcgcgtcgag ctgacgccgc tgcgcctgct ctggcaggcg tggcgggcgc ggcgccgggc
     1081 gcggcgcggc ggctgaaatc ggcgcggcac ggcgcttgcc gctgcagcgc cggccggcgg
     1141 ctcggctaga atccgcgccc ccggcgccgg tgcgcccgcc cgaacgaggc cccgagatga
     1201 actacgaccc gcagtacgct cccgcccccg acctgctgcg cgagcgcgtg atcctgatca
     1261 ccggcgccgg cgacggcatc ggccgggcgg tcgcgcgcgc ctgcgccgcg cacggcgcga
     1321 ccgtgatcct gagcggccgc acgaagaaga agctcgaagc ggtctacgac gagatcgaaa
     1381 gcgccggcca cccgcagccg gtgatccacc cgctgaacct cgaaagcctg acgccgaccg
     1441 atgtcgacac catcgccgag gcgatcgacc agaacttcgg ccggctcgac ggcctgctgc
     1501 acaacgccgg ctgggtcggc tcgctgacgc cgatcgccaa ctacgagccg gagctgtggc
     1561 tgcgcgtcgt gcaactgaac ctgagcgcgc ccttcctgct gacgcaggcg ctgctcggcc
     1621 tgctgaaccg cgcaccggcg gcctcggtgg tgttcacgac cgacgccgtc gcgcagcgcg
     1681 cgcaggccta ttacggcgcc tacgccgcgg ccaagggcgg catcgaggtg ctcgcgcgca
     1741 tgctcgcctc ggagctcgaa gccaacacgc gcatccgcgt caacaccatc gaccccggcg
     1801 aggtgcgcac caacctgcgc atccgcacct tccccggcat cacgcccgag aactggccgc
     1861 ggccggaagc cctggtcaac gcctggctgt ggctgctcgg cccggactcg gacggcgtgc
     1921 gcggacagct cgtgcaggcg cagccgcgct gagcgccgcg cgggtggcga cacgcgccgt
     1981 gcgccgctgc cgccctcacg acggtccggc ttcgagcccg cccgcgcgcg gcacgaagcg
     2041 caccggcgcc tggccgcgcg cggcgcgctt gtcggcgagc agcagcggtg cgcagcgcct
     2101 gcggtcgcgg tagatcgcca cgcagtccag acactggaag cagtcggcgt agcggatcgc
     2161 gccgtcgcgg cggatcgcgc cgtactcgca gctgcgccgg cacagctggc aggggctgcc
     2221 gcactcggcg cgccgcggca gccacgcgaa cacgcgcacg cgcccgagca gcgccagcgc
     2281 cgcgccgagc ggacacagcc agcggcagaa ggccttgtag aacaccaggt tcagcgcgat
     2341 cagcgccagc gcccacgcga cgaacggcag gctgcgcacg aagccgagcg tgatcgcggt
     2401 cttgaacggc tcgatctcga ccagccggtc ggagagcgcc ggcgcgaacc acgccgcggc
     2461 gaggatcagc gccagcaccg catatttgac cggcatcagc cgggcatcga gcgcctccgg
     2521 cacgcgccgc ggctgcaggc cgagcgcgcg cgcaccgagc gccgccagct cctgcagcgc
     2581 gccgaacgga cacagccagc cgcagaaggt gccgcgcccc cagaccacca gcgccagcgc
     2641 cgtgtacgcc cagaccagca ccgtcatcgg gtcgttgagc aggaagtcga gcccgcgccc
     2701 ggcgcgcagc gcctggatca cggccgtcag gttgacgatc gacagctgcc cctgcgcgta
     2761 ccagccgatg aaaccgagcg tgaagacgag ccacagcgtg cgcacccggc gcaggcgcgt
     2821 ggcgtcgtgc gtgagccacg ccggtcgcgc cagcagcagc gtcagcagca cgaagccggc
     2881 gagcaggatc agcagctcgc cggcacgctc ggcccagacc gcgcgccagc cgctcagcgg
     2941 ctgcggggcc tgcggtgcgc gctcgacgta gcgtgccggc agggccagtt cgaggcccag
     3001 gtcgcggctg acgcgctccg gatacagcag gccgtgggcg cgcgtcgtgc gcaccgacag
     3061 ttcgaccggc cgcgccggat cgagcccggc ctgcgccagc acgcggaaga acttcacggt
     3121 atcgaagtcc ggcagcccgg cgacgcgcac gcgctcgtcg aggtccatgt cgcgcagccc
     3181 gagcggcagc ccgccctgcg cgagcccgag ccggtcgggg atggcattgc gcacgaagtc
     3241 cggcccggcg aagccgtagc gcccgcgcga gaacgccagc agcaccagat cccccggctc
     3301 cagcgtgcgc gcgatgcgcg cccaggcgtc gtcgcccagc agcgccgcat gcacgcccgg
     3361 cacgttcagc agcgtcgcgc agaactccag atacagcgcc tccggggcgg ccagcgcctc
     3421 cgggtcctga ccctcgccga ccgtgccggc gaaggcccgc tcgacctcgc ggttcgagta
     3481 ctggcggcag gccaccagcc ccttgtcctt cagtccggcc cagtcgaggc cggcatcgag
     3541 cacctcactg cggatggtgc cggcgcgtgc cggactgccg ccgccgatgc cgagcttcgc
     3601 gcgcgccacg gccagcgcgg cgttgacgat cgagtcgttg acgatgcgca ccgaggccgt
     3661 cgccttcgag accccgtcga tgtaggcatt ggcgctgtcg agccgcacgc cgccgttggt
     3721 gctgctggtg accttgatgt tgcggttcag cccgaggccg acgtactggc gcacgaaggc
     3781 cgcgagcggc tccggcccga ggccgtcgag gaataccggc tcgtgctgcg agatcacgcg
     3841 cacgtcgcgg aactgcccgt ccggcgcgat ctcgaccagc aggttgaccg gcgtgcccga
     3901 gaagcccggg atcggcgcga agtcgatcga ctcgaacagc cagccttcaa gttccggata
     3961 cggcccgttc atcctggaca gcggccagac cggcagctcg cgcgcctgct ccccgacgct
     4021 gtacggcggc ggatagatct gtccgagcgt cgtacgggtc atgacgccgg ccccggcaag
     4081 gccggcggac aggatcagcc agaggccgag caggccggcg acgaagcggg ctgcggcagc
     4141 ggggatgcgg ggcaacggca tcggtacgga cctgcacgga cggcgggggg aagcgcaggc
     4201 gcgggcaccg gcggtcgatc cgggcagctt cgatccgatc cgatccgtcc cgctccgcgc
     4261 gcggcctgcg catcgtcggc agtccgcatc cggcctgcaa gggcgctctc aggatgtgcg
     4321 cctgcacatc caggacttgc cgaagcgtgc cgctgtagac ccgatccgtg cgcaggcgcc
     4381 gggcgcgccg cctgcgcacg cttgcctcac tcgccgtcgt cgcgctgcgg cccgcgcttc
     4441 aagccgagcc gcggacgctg ggccgggctg accggcgccg gagcgcccct gccaccgctg
     4501 ccaccgctgc caccgccgcc gcgccggcgc tcggcgtcct cggcgaagcg ccggccgcgc
     4561 gcgccggcgg c
//
LOCUS       DAOQIE010000299         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11002_length_4571_cov_5.690434, whole genome shotgun sequence.
ACCESSION   DAOQIE010000299 DAOQIE010000000
VERSION     DAOQIE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_4571_cov_5.690434"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK144_15350"
     CDS             complement(<1..276)
                     /locus_tag="PK144_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67152.1"
                     /translation="MTMLHVERLGGLAGFGGPRARIRSCGCIELADLPDAARASVEAL
                     FRKPPPKRRSASAPADGFRYRLRCEIDGEERSIEVAETAVPAAVSACV"
     gene            complement(309..1418)
                     /locus_tag="PK144_15355"
     CDS             complement(309..1418)
                     /locus_tag="PK144_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNF67153.1"
                     /translation="MSRPSRPETAPCAARLTPHHRHAAGCGCFIIPTRVLDRFAQDEQ
                     LPEAQRKSFADAASIETEWRKVRSASQELMRMAHRVLPIGMTALAAAAPPVVSVFDCR
                     HGMALPGTPFCDPAHATDETARRTFAETTAMAEFLRLAFNRNSLDDAGMGLLSSIHFS
                     VGYNNAFWNGLQMTYGDGDGNIFIDFTRSNDVIAHELTHGLTEYTAGFAYRNQAGGLN
                     ESVSDVFGSMFRQWRAGQSVQQADWLIGRDIMGPGALARGYTCLRDLANPGAEHCLSP
                     QPAHFDQYRNGMDPHDSSGIPNQAFYRAAMAIGGRSWEKTGRIWYHALTGYRPSPNLS
                     MRSFANRTRSLARQIFGSEPSIRRAVDQAWLAVGL"
     gene            complement(1445..>1662)
                     /locus_tag="PK144_15360"
     CDS             complement(1445..>1662)
                     /locus_tag="PK144_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67154.1"
                     /translation="TRLIAAGCGIRKCPTAGKAGHVPEWHFDSFHPACLQGRNPQAGR
                     ERHIDDSGLNAAAVGCNTAATFQKPDA"
     assembly_gap    1663..1762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1782..2927)
                     /locus_tag="PK144_15365"
     CDS             complement(1782..2927)
                     /locus_tag="PK144_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525686.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF67155.1"
                     /translation="MPQHERAPSSWPWPYPRWQRWLIELVSLAVILGLLALAVTTVRD
                     NLRAQQIASGFGFLQREAGFAIAETPIAYSPADSYARAIAVGLVNTVRVSLPALFGAT
                     VLGVLLAVALRSAQPLARRGARVYVECFRNTPLLLQLFLWYALLTTLLPPPRQAWQPL
                     AAVFVSNRGLYLPVPLEPGPWLWALAVALPALVAALRLRGRLLHTRRRGRLLAPLALF
                     ALPVLATLWLGGASAGLSQPVLRGFNFVGGTVLTPEFAALGLGLTLYSASYIAEIVRG
                     GIDAVPAGQREAALAVGLTPVQALRLVVLPQAVRVALPPYAGQALSLIKNSSLAVAIG
                     YPDLLSIASTTINQTGQAIEGIAIVMAAYLSVNLAVAGLIALWGRGR"
     gene            complement(2928..3941)
                     /locus_tag="PK144_15370"
     CDS             complement(2928..3941)
                     /locus_tag="PK144_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNF67156.1"
                     /translation="MPMFRFAVAVLVLAAAAAPAPAGETLAAVLARGSLRCGVNVSVP
                     GFSSPDSQGRWTGLDADICRAVAAAVLGSADKVEFVPLNAQQRFTALQSGEIDLLSRN
                     TTWTLGRAAGLKLRFGPVVFYDGQGFMVAKTLGVKSATELDGASVCVQSGTSTEQSVA
                     DWFRARHMSFKPVVMDNFDELNRAFFAHRCDVYTTDRSGLASIRATMAGSPEDYAILP
                     EAISREPLAPAVLQKDAEWAEAVFWSVSALIEAEERGVSSQNVRAMRDSGDPALGRML
                     GASSDLGDALGLRADWAYQAILQVGNYGEIYERNLGAPLGLARGPNALWTQGGLLYAM
                     PVR"
     gene            4173..4514
                     /locus_tag="PK144_15375"
     CDS             4173..4514
                     /locus_tag="PK144_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNF67157.1"
                     /translation="MESSYPVTLNQDSKNVALLVWIGTIFFSFVPALIVYLIKKDDGY
                     VQDQAREALNWAITVMIGFLLARILAWVLIGALAYPVVALVNLIFCVMGAVAATQGKA
                     FRVPFALRLIK"
BASE COUNT          675 a         1557 c         1594 g          645 t
ORIGIN      
        1 cacgcaagcc gaaaccgccg ccggcaccgc ggtctccgcg acctcgatgc tgcgctcctc
       61 gccgtcgatc tcgcagcgca gccggtagcg gaaaccgtcg gccggggcgg aggcgctgcg
      121 gcgtttcggg ggcggttttc tgaacagggc ttcgacgctg gcgcgcgccg cgtccggcag
      181 gtcggcgagt tcgatgcagc cgcagctgcg gatgcgcgcg cgcggaccgc cgaagccggc
      241 cagtccgccg agtcgctcga cgtgcagcat cgtcatcgcg aatctcccgg tgcgcgtgcg
      301 ggcgccgatt ataggccgac cgccagccag gcctgatcga cggcgcggcg gatcgacggt
      361 tcgctgccga agatctggcg tgcgaggctg cgcgtgcggt ttgcgaagct gcgcatcgac
      421 aggttcgggc tcgggcggta gccggtcagc gcgtggtacc agatgcggcc ggtcttttcc
      481 cagctgcgcc cgccgatcgc catcgccgcc cgataaaagg cctggttcgg gatgccgctg
      541 ctgtcgtgcg ggtccatgcc gttgcggtac tggtcgaaat gcgccggctg cggcgacagg
      601 cagtgctcgg cgcccggatt ggccaggtcg cgcaggcagg tgtagccacg cgccagcgcg
      661 cccggtccca tgatgtcgcg tccgatcagc cagtcggcct gctgcacgct ctggccggcg
      721 cgccattgcc ggaacatcga accgaatacg tccgacacgc tttcattgag tccgccggcc
      781 tgattgcgat aggcgaagcc ggccgtgtat tcggtcaggc cgtgcgtcag ttcgtgcgcg
      841 atcacgtcat tggagcgcgt gaaatcgatg aagatgttgc cgtcgccatc accgtaggtc
      901 atctgcaggc cattccagaa ggcgttgttg tagccgacac tgaaatggat cgacgacagc
      961 aggcccatgc cggcatcgtc gagcgaattg cggttgaagg ccaggcgcag gaattcggcc
     1021 atggccgtgg tttcagcgaa ggtgcggcgg gcggtctcgt cggtggcatg ggcgggatcg
     1081 cagaagggcg tgccgggcag cgccatgccg tggcggcagt cgaagacgct caccaccggc
     1141 ggcgcggcgg cggcgagcgc ggtcatgccg atcggcagca cgcggtgcgc catgcgcatc
     1201 agctcctggc tggcgctgcg caccttgcgc cattcggttt cgatgctcgc ggcatcggcg
     1261 aaggatttgc gttgtgcctc gggcagttgc tcgtcctgcg cgaagcggtc gagcacccgg
     1321 gtcgggatga tgaagcagcc gcagccggcc gcgtggcggt gatggggcgt gagtctcgcg
     1381 gcgcacggtg cggtttcggg acgggacgga cgggacatgt tcggactcct gatggcggtg
     1441 gccgtcatgc gtccggcttc tggaacgtgg ccgccgtgtt gcaacccacg gccgcggcgt
     1501 tcaggccgga atcgtcgatg tgccgttcac gtccggcttg cggattcctc ccctgcagac
     1561 aggcagggtg gaatgaatcg aaatgccatt cgggcacgtg accggctttt ccggctgtcg
     1621 gacatttgcg gatgccgcag ccggcagcga tgagcctagt gcnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnaagcccgc ggcgcggcgc atcagcgtcc cctgccccac
     1801 agcgcgatca gccccgcgac cgcgaggttc acgctcagat aggccgccat gacgatcgcg
     1861 atgccttcga tcgcctgccc ggtctggttg atcgtcgtgc tggcgatcga gagcaggtcc
     1921 ggatagccga tcgcgaccgc gagcgagctg ttcttgatca ggctcagcgc ctgaccggca
     1981 tacggcggca gcgcgacgcg caccgcctgc ggcagcacca ccagccgcag cgcctgcacc
     2041 ggcgtcagcc ccacggccag cgccgcctcg cgctggccgg ccggcacggc gtcgatgccg
     2101 ccgcgcacga tctcggcgat gtagctcgcc gagtacagcg tgaggccgag cccgagggcc
     2161 gcgaactccg gtgtcagcac cgtgccgccg acgaagttga agccgcgcag caccggctgc
     2221 gacagtccgg cgctcgcgcc gccgagccac agcgtcgcca gcaccggcag cgcgaacagc
     2281 gcaagcggcg ccagcagccg gccgcggcgg cgcgtgtgca gcagccggcc gcgcagcctc
     2341 agtgccgcga ccagcgccgg cagcgcgacc gccagcgccc acagccacgg ccccggttcg
     2401 agcggcaccg gcaggtacag gccgcggttg ctgacgaaca ccgccgccag cggctgccag
     2461 gcctgacgcg gcggcggcag cagcgtcgtc agcagcgcgt accacaggaa cagctgcagc
     2521 agcagcggcg tgttgcggaa gcactcgacg tagacgcgcg cgccgcggcg ggcgagcggc
     2581 tgcgcgctcc gcaaggccac ggccagcagc acgccgagca cggtcgcgcc gaacagcgcc
     2641 ggcagcgaca cgcgcacggt gttgacgagg ccgacggcga tggcacgcgc atagctgtcg
     2701 gccggcgaat acgcgatcgg tgtctcggcg atcgcaaagc cggcctcgcg ctgcaaaaaa
     2761 ccgaagccgc tcgcgatctg ctgcgcgcgc aggttgtcgc gcaccgtcgt caccgcgagc
     2821 gccagcaggc cgaggatcac ggcgagtgac acgagctcga tcagccagcg ctgccagcgc
     2881 ggatacggcc acggccacga cgagggcgcg cgctcgtgct ggggcatcta gcgcaccggc
     2941 atcgcgtaca gcagcccgcc ctgcgtccac agcgcgttcg ggccgcgcgc gaggccgagc
     3001 ggggcgccga ggttgcgttc gtagatctcg ccgtaattgc cgacctgcag gatggcctga
     3061 tacgcccagt cggcgcgcag ccccagcgca tcgccgagat cgctgctggc gccgagcatg
     3121 cgcccgagcg ccggatcgcc gctgtcgcgc atcgccctga cgttctgcga gctgacgccg
     3181 cgctcctcgg cctcgatcag cgcgctgacg ctccagaaca ccgcctcggc ccactccgcg
     3241 tccttctgca gcacggccgg tgccagcggc tccctggaga tggcctcggg caggatcgcg
     3301 tagtcctcgg ggctgccggc catggtcgcg cggatcgacg ccagccccga gcggtcggtc
     3361 gtgtagacat cgcagcgatg cgcgaagaag gcgcggttca gctcgtcgaa gttgtccatc
     3421 accaccggct tgaagctcat gtgccgggcg cggaaccagt cggcgaccga ctgctcggtg
     3481 ctggtgccgc tctgcacgca gacgctggca ccgtcgagtt cggtggcgct cttcacgccg
     3541 agcgtctttg cgaccatgaa gccctgaccg tcgtagaaca ccaccggccc gaagcgcagc
     3601 ttcaggcccg ccgcgcgccc gagcgtccag gtggtgttgc gcgacagcag atcgatctcg
     3661 cccgactgca gcgccgtgaa gcgctgctgc gcgttcagcg gcacgaactc gaccttgtcg
     3721 gcgctgccga gcacggccgc ggcgaccgcg cggcagatgt cggcatcgag tccggtccag
     3781 cgcccctgac tgtccggcga cgagaacccc ggcacgctga cattgacgcc gcagcgcagg
     3841 ctgccgcgcg cgagcacggc cgcgagggtt tcaccggccg gggccggcgc ggcggcggca
     3901 gcgaggacga gaacggcgac ggcgaagcgg aacatcggca tggcaggact ccggcggcgg
     3961 cagcagacat gcgcccgact ctagcccgaa gccccgcccg cgcgctgcca gcggccggcg
     4021 cgcgaacttc gccggccgcg gcgcagtcgc agaatgcaag gcccggcgcg cccctgtccg
     4081 tgcgcggccc gccggtccgg cctgtccgat ccatcatccg ccaacccggc gcgcccgccc
     4141 cgtgccgcgc gccccgagtg aggaaacccg tcatggaaag ctcatacccg gtgacgctga
     4201 atcaggattc gaagaacgtc gcgctgctgg tctggatcgg cacgatcttc ttcagcttcg
     4261 tgcccgcgct gatcgtgtat ctgatcaaga aggacgacgg ctatgtgcag gatcaggcca
     4321 gggaggcgct gaactgggcg atcaccgtca tgatcggctt cctgctcgcc cgcatcctcg
     4381 cctgggtcct gatcggcgcg ctggcctacc cggtcgtcgc gctggtgaac ctgatcttct
     4441 gcgtgatggg cgccgtcgcc gccacgcagg gcaaggcgtt ccgcgtgccg ttcgcgttgc
     4501 ggctgatcaa gtgagggcgg acctggcgcg gcgccggggc gggccgcgcc acggaagttg
     4561 gccagcggcg g
//
LOCUS       DAOQIE010000300         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11007_length_4569_cov_10.156402, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000300 DAOQIE010000000
VERSION     DAOQIE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11007_length_4569_cov_10.156402"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1348)
                     /locus_tag="PK144_15380"
     CDS             complement(77..1348)
                     /locus_tag="PK144_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF67158.1"
                     /translation="MSTDHQKQPDSQKASASASAPVSATPVDARRRKPLLLAVALAVA
                     AGAAGSGAWWWSTARHLETTDDAYVAGDVIPVTPRVAGTVLAVRADDTDVVVAGTPLV
                     DLDPADARVALDAAEADLARAVRNVRQLFATRDALRSTVANREADLAKAKEDLARRQK
                     LSGNGAISAEEMEHARTALRSAEASLANVRDQLSATEVLIENTAVDTHPDVKRAAAKV
                     EEAALALARTAIVAPVDGQVARRVVQVGARVAAGTPLLALVPLDRLWVDANFKESQLA
                     RLRIGQPVTLSADVWGSHVEYRGKVIGLGAGTGGAFALLPAQNASGNWIKVVQRVPVR
                     IELDHDQLVAHPLRVGLSMVVEVDVSRQDGRQIADSQPRSAPVVQTGGDGSNLDGIRD
                     RIREIIAANAGNGVAVAEPVADARRPGAGRG"
     gene            complement(1383..2918)
                     /locus_tag="PK144_15385"
     CDS             complement(1383..2918)
                     /locus_tag="PK144_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNF67159.1"
                     /translation="MTKTSAVPVSASPSPIRPARRAGLGLLAAALLAACADPSGIAGQ
                     SAYTDASALAAQRTLTEARAATAAWPTEGWWRAWNDPGLDRLIGDALSDNPNLKLAEA
                     RLRNAQALAGLADANRGPRLDAGATLSRERLPEGYIYPAPLGGSTQTDARLGLSFSWE
                     LDLWGRRQAEAAAALDRASAAEVEAQAARLMLAVAIARGWNDLDGRYAQHDVAAAEVE
                     QRRQVLELTRQRVAAGLDSSVELKQAEGAVPAARQQLAATEEGIRLARTQLAALAGRG
                     PDFGLALPRPALRAPEALALPSTIPAELLGRRPDLIAARLRAEAAAKDSTAARAAFYP
                     NVNLAAFAGFQALGLSHLVEAGNRSLSVTPAVTLPIFDSGRLRANLAASEAGGDIAIE
                     QYNAALVDGLHEVADALASARSLETQQAEQRAAQASAEEALRLANLRYREGLSNYLTV
                     LSAQTEVLAQRRLGATLRTRRNDVSLALVRALGGGYQPAAGVATASTAAGPRPVTNPS
                     TRS"
     gene            complement(2998..3477)
                     /locus_tag="PK144_15390"
     CDS             complement(2998..3477)
                     /locus_tag="PK144_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF67160.1"
                     /translation="MTDPRPDPTADTLLYTPENYTPTESVGYLLGQAKGRITAAIDAE
                     MAPYDITGAQWITLLHLSCTEVETAGELCKRACVDTGSMTRMLDRLEKKGLVQRVRCC
                     EDRRVVRLRMTEAGEKLAALMPDVASRVLNHHLRGFSRDELELLKSLLRRIIANADG"
     gene            4197..>4569
                     /locus_tag="PK144_15395"
     CDS             4197..>4569
                     /locus_tag="PK144_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF67161.1"
                     /translation="MSGLHRITPLARTALQHGGRCFAIAALLICAAVPAHAGRVHHTH
                     AGKSASAAGSAQGAGDHDQIGVASYYSSRFSGRRTASGERFDPSAMTAAHMTLPLGTR
                     VRITNLKNGQSVVVRINDRGGF"
BASE COUNT          630 a         1547 c         1677 g          715 t
ORIGIN      
        1 gggccggtgc gggggggctg cgggacggac gacgaggcgg aaggcgcggc ggcggacatc
       61 gcggggcgct cccggctcag ccgcggccgg cgccgggccg gcgggcatcg gcgaccggtt
      121 cggccacggc gacgccgttg ccggcgttgg cggcgatgat ctcgcggatg cggtcgcgga
      181 tgccgtcgag gttgctgccg tcgcccccgg tctgcacgac cggggcgctg cgcggctggc
      241 tatcggcgat ctggcgaccg tcctgacggc tcacgtcgac ctcgacgacc atcgacaggc
      301 cgacgcgcag cggatgcgcg acgagctggt catggtcgag ttcgatgcgc accggcacgc
      361 gctgcacgac cttgatccag ttgccgctgg cgttctgcgc cggcagcagc gcaaacgccc
      421 cgccggtgcc ggcgccgagg ccgatgacct tgccgcggta ctcgacgtgg ctgccccaga
      481 catcggccga cagcgtcacc ggctggccga tgcgcaggcg cgcgagctgg ctttccttga
      541 agttcgcatc gacccacagc cggtcgagcg gcaccagcgc cagcagcggc gtgccggcgg
      601 cgacgcgcgc gccgacctgc acgacgcggc gcgcgacctg gccgtcgacc ggcgcgacga
      661 tcgcggtgcg cgcgagcgcg agcgccgcct cctcgacctt ggcggcggcg cgcttcacgt
      721 ccgggtgcgt gtcgactgcg gtgttctcga tcagcacctc ggtcgccgag agctggtcgc
      781 ggacgttggc gaggctcgcc tcggcgctgc gcagcgccgt gcgcgcgtgc tccatctcct
      841 cggccgagat cgcgccgttg ccggagagct tctggcgccg tgcgaggtcc tccttcgcct
      901 tcgcgagatc ggcctcgcgg ttggcgaccg tcgagcgcag cgcgtcgcgg gtcgcgaaca
      961 gctggcggac gttgcgcacg gcgcgggcca gatcggcctc ggcggcatcg agcgcgacgc
     1021 gcgcatcggc cggatcgaga tcgaccagcg gcgtgccggc gacgaccacg tcggtgtcat
     1081 cggcgcgcac cgcgagcacg gtgccggcga cgcgcggcgt caccgggatc acgtcgccgg
     1141 cgacgtaggc gtcatcggtc gtctccagat gacgggccgt gctccaccac cacgcaccgc
     1201 tgccggcggc accggcggcg acggcgagcg cgacggcgag cagcagcggc ttgcgccggc
     1261 gcgcatcgac cggcgtggcg gagacggggg cggatgcgga tgcggatgct ttctggctgt
     1321 ccggctgctt ctggtgatcg gtgctcatga tcggggggct ccggggggcg ggcggatgcg
     1381 gatcagctgc gggtgctggg gttcgtgacc gggcgcgggc cggcggcggt gctcgccgtg
     1441 gccacgccgg cggccggctg gtagccgccg ccgagcgcac gcaccagcgc cagcgacacg
     1501 tcgttgcggc gggtgcgcag cgtcgcgccg agccggcgct gcgccagcac ctcggtctgc
     1561 gcgctgagca cggtcaggta gttggaaagc ccttcgcggt agcgcaggtt ggcgaggcgc
     1621 agggcttctt cggcgctcgc ctgcgcggcg cgctgctcgg cctgctgcgt ttccagcgaa
     1681 cgggcgctgg cgagcgcatc ggcgacctcg tgcaggccgt cgaccagcgc ggcgttgtac
     1741 tgctcgatgg cgatgtcgcc gccggcctcg ctggcagcga ggttggcgcg cagccgcccc
     1801 gagtcgaaga tcggcagcgt cacggccggc gtcaccgaca gcgagcggtt gccggcctcg
     1861 accagatgcg acaggccgag cgcctggaag ccggcgaagg cagcgaggtt gacgttcgga
     1921 tagaaggccg cgcgcgccgc cgtgctgtcc ttcgctgccg cttcggcgcg cagccgcgcg
     1981 gcgatcaggt ccgggcgccg gccgagcagc tcggccggga ttgtcgatgg cagcgccagc
     2041 gcctcgggcg cgcgcagcgc cgggcgcggc agcgcaaggc cgaagtccgg accgcggccg
     2101 gcgagcgcgg cgagctgcgt gcgcgccaga cggatgcctt cctcggtcgc cgcgagctgc
     2161 tggcgcgcgg ctggcaccgc gccttcggcc tgcttcagct cgaccgacga atccagaccg
     2221 gcagcgacgc gctggcgcgt cagctccagc acctgccggc gctgctcgac ctcggccgcg
     2281 gccacgtcgt gctgcgcgta gcggccgtcg agatcgttcc agccgcgggc gatcgccacc
     2341 gccagcatca gccgcgcggc ctgcgcctcg acctcggcgg cgctggcgcg atcgagcgcc
     2401 gcggcggcct cggcctgacg gcggccccac agatccagct cccacgagaa gctcaggccc
     2461 agacgggcgt cggtctgcgt gctgccgccg agcggcgccg ggtagatgta gccctcgggc
     2521 aggcgctcgc ggctgagggt ggcgccggcg tcgaggcgcg ggccgcggtt ggcatcggcg
     2581 agcccggcga gcgcctgggc gttgcgcagc cgcgcctcgg cgagcttcag gttcggattg
     2641 tcggacagcg catcgccgat cagccggtcg agcccggggt cgttccacgc ccgccaccat
     2701 ccttcggtcg gccaggccgc ggtcgcggcg cgcgcctcgg tgagcgtgcg ctgcgcggcg
     2761 agcgcgcttg catcggtata cgcagactgg ccggcgatgc cggagggatc ggcgcaggcg
     2821 gcgagcagcg ccgcagccag caggcccagg ccggcgcggc gggcagggcg gatcggggaa
     2881 gggcttgcgg agacgggaac ggcgctggtc tttgtcatgg cggtttctgc ctgtctttta
     2941 tctgcctgag cagttattgc gacgcacgcg gtgcgacagg gtgacgggac cggcggttca
     3001 gccgtcggcg ttggcgatga tccggcgcag cagggacttc agcagttcga gttcatcgcg
     3061 cgagaagccg cgcaggtgat ggttcagcac gcgcgaggcg acatcgggca tcagcgccgc
     3121 gagcttctcg ccggcttcgg tcatgcgcag gcgcacgaca cgacggtcct cgcagcagcg
     3181 cacgcgctgc accagtccct tcttttcgag ccggtcgagc atgcgcgtca tcgagccggt
     3241 atcgacgcag gcgcgcttgc agagttcgcc ggcggtctcg acctcggtgc aggacaggtg
     3301 cagcagcgtg atccactgcg cgccggtgat gtcgtacggc gccatctcgg cgtcgatcgc
     3361 cgccgtgatc cggcccttgg cctgaccgag caggtaaccg acgctctcgg tcggcgtgta
     3421 gttctcgggg gtgtacagca gcgtgtcggc tgttggatca ggccttggat cggtcatggc
     3481 ggcgttctgg gtggacggta tcgacatggg tcggcaattt aactgcctat gcagagaatg
     3541 gcaatgcaga aactgcacgt ccgactgtac agaatcgtat ggatgttggc ctgacatcct
     3601 gatttccatg cagatctgca cgaatactgg tcagaccggg cgccggcccg cagcgaagcc
     3661 ctgggatccg gcaggcattg cggcctgcat ccgcttccgg ttcagggtct ggttccggtc
     3721 ctgattcagg attgtgtcgt gtttgacacg gatggcgctt caggcgtcat cgtcgttcag
     3781 cgtcaccgat ttaatacaga taaaaatata tctgtatgga tgattccgta tccgtaaacg
     3841 cacgtaaacc gatgctcaaa ccgggctgat ccgggaaagc ggccattgcg tggccggcgc
     3901 gctgcgccgc tgcaggcaga atccggaagt cctttccata cggtaacttg cgcagattcc
     3961 tgcctttcgg ggccggccgg tggcgcgcag tgcgtgccgg ttcgtgccgg cgcggctgcc
     4021 atctgaaacg cagatgcaaa cagcgatatt tgtatctttt aattttcaga catgcacgct
     4081 atgtcattga tgggcaagca ttgttgtatt tcagacaaaa tttgaccaat gtgtttttcc
     4141 atgctgcgga atcgggtact tgagacggcc aaaccacctt ctcggggatg cagcccatgt
     4201 ccggactaca tcgcatcact cctcttgccc gcaccgccct gcagcacggc ggccgttgtt
     4261 tcgccatcgc cgcgctgctg atctgcgccg ccgtccccgc ccacgccggc cgggtgcacc
     4321 acacccacgc cggcaagagc gcatcggccg ccggaagcgc gcagggcgcg ggggaccatg
     4381 accagatcgg cgtggcctcg tactacagca gccgcttcag cggccggcgc acggcctcgg
     4441 gcgagcgctt cgacccctcg gccatgaccg ccgcgcacat gacgctgccg ctcggcacca
     4501 gggtgcgcat caccaacctg aagaacggcc agagcgtcgt cgtgcgcatc aatgaccgcg
     4561 gcggcttcc
//
LOCUS       DAOQIE010000301         4543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11105_length_4543_cov_7.988191, whole genome shotgun sequence.
ACCESSION   DAOQIE010000301 DAOQIE010000000
VERSION     DAOQIE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11105_length_4543_cov_7.988191"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2339)
                     /gene="acnD"
                     /locus_tag="PK144_15400"
     CDS             complement(<1..2339)
                     /gene="acnD"
                     /locus_tag="PK144_15400"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNF67162.1"
                     /translation="MNTAYRKLLPGTKLDYFDARAAVDEIKAGAWATLPYTSRVLAEN
                     LVRRCEPAMLRDSLSQLIERKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQG
                     GDPAKVNPVVPTQLIVDHSLAVEYGGFDPEAFDKNRAVEERRNEDRFHFIDWTRTAFQ
                     NVDVIPAGNGIMHQINLEKMSPVIQVRDGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAETVMLGRASMMRLPDIVGVRLTGKRQPGITATDIVLALTEFLRKERVVGAYVEFF
                     GEGAESLSIGDRATISNMCPEYGATAAMFYIDGQTIDYLKLTGREPEQVALVEQYAKT
                     LGLWGDALASAEYERVLTFDLSTVVRNMAGPSNPHKRLPTSALAERGIADEAKLAQAR
                     AEEADGLLPDGAVIIAAITSCTNTSNPRNVVAAGLLARKANQLGLTRKPWVKSSFAPG
                     SKVAKLYLEESGLLPELEKLGFGIVAYACTTCNGMSGALDPKIQQEIIDRDLYATAVL
                     SGNRNFDGRIHPYAKQAFLASPPLVVAYALAGTVRFDIEQDALGFDADGRAITLKDLW
                     PSDEEIDAIVASSVKPEQFKQVYIPMFDLGTVEAAKSPLYDWRPQSTYIRRPPYWEGA
                     LAGERTLKGMRPLAVLPDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHR
                     GDHLTAQRATFANPKLVNEMVVVDGKVKQGSLARVEPDGTVMRMWEAIETYMDRRQPL
                     IIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRT"
     gene            complement(2342..3496)
                     /gene="prpC"
                     /locus_tag="PK144_15405"
     CDS             complement(2342..3496)
                     /gene="prpC"
                     /locus_tag="PK144_15405"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNF67163.1"
                     /translation="MTESTQVLPKAKKSVALSGTAAGNTAICTVGRTGNDLNYRGYDI
                     LDLADKCEFEEIAHLLIHHKLPTASELKAYKAKLKALRGLPLPVKAVLEQIPAAAHPM
                     DVMRTAVSTLGCVLPEKDDHNSAGARDIADRLIASLGSMLLYWYHYSHNGKRIEVETD
                     DESIGGHFLRLLHGERPSELHVRSMHTSLVLYAEHEFNASTFTGRVIAGTSSDLYSAI
                     TGAIGALRGPKHGGANEVAFEIQQRYDNPEEAEADIRERVERKEVVIGFGHPVYTISD
                     PRNKVIKEVARTLAADAENFKLFDIADRLETVMWDAKKMFPNLDWFSAVSYHLMGVPT
                     EMFTPLFVIARTTGWAAHVIEQREDGKIIRPSANYIGPEDRPFVAIEDRK"
     gene            complement(3530..4417)
                     /gene="prpB"
                     /locus_tag="PK144_15410"
     CDS             complement(3530..4417)
                     /gene="prpB"
                     /locus_tag="PK144_15410"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNF67164.1"
                     /translation="MSELKSAGARFRAAVREERPLQVVGAVNAYFARLAEHSGYKALY
                     LSGGGVAACSCGIPDLGITTMEDVLIDARRITDVTDLPLLVDIDTGWGGAFNIARTVR
                     ELTRAGVAAVHIEDQVAQKRCGHRPNKAIVSQAEMVDRVKAAVDAKTDSNFVIMARTD
                     ALAVEGLQSAIDRACACVEAGADMIFPEAMTDLGMYRQFAEAVGVPVLANITEFGSTP
                     LFTTDELGANGVSLVLYPLSAFRAASKAALNVYQQLRASGTQQGVVDTMQTRAELYEH
                     LGYHAYEQKLDALFSKDAK"
BASE COUNT          684 a         1459 c         1613 g          787 t
ORIGIN      
        1 gtgcggtgga tgcgctcgaa gccctcggcg acgatggcct cgacgccggc cagacgcacg
       61 cccttggccg cccagtcacg cgaactgccc tgaccgtaat cggcaccggc gatgatgatc
      121 agcggctggc ggcgatccat gtaggtctcg atcgcctccc acatgcgcat gaccgtgccg
      181 tccggctcga cgcgcgcgag cgaaccctgc ttgaccttgc catcgacgac gaccatctcg
      241 ttgacgagct tcgggttcgc gaaggtcgcg cgctgggcgg tcaggtgatc gccgcggtgc
      301 gtcgcgtagg aattgaagtc ctcctccggc aggcccatct tcgccaggta ttcaccggcg
      361 gcgctgtcga gcaggatcgc gttcgacggc gacaggtgat cggtcgtgat gttgtccggc
      421 agcaccgcga gcggacgcat gcccttcagg gtgcgctcgc cggccagcgc gccttcccag
      481 tacggcggac ggcggatgta ggtcgactgc ggacgccagt cgtacagcgg gctcttggcg
      541 gcctcgacgg tgccgaggtc gaacatcggg atgtagacct gcttgaactg ctcgggcttg
      601 accgagctgg ccacgatggc gtcgatctcc tcgtcgctcg gccagagatc cttcagcgtg
      661 atcgccctgc catcggcatc gaaaccgagc gcgtcctgct cgatgtcgaa gcgcacggtg
      721 ccggcgagcg cgtaggcgac gaccagcggc ggcgaggcga ggaaggcctg cttggcgtac
      781 gggtggatgc ggccgtcgaa gttgcggttg ccggagagca cggcggtcgc gtacaggtcg
      841 cggtcgatga tctcctgctg gatcttcgga tcgagcgcgc cggacatgcc gttacacgtc
      901 gtgcaggcat aggcgacgat gccgaagccg agcttttcga gttccggcag cagaccggac
      961 tcttcgagat acagcttcgc gaccttcgag cccggcgcga acgaggactt cacccagggc
     1021 ttgcgcgtca ggccgagctg attggccttg cgcgccagca ggccggcggc aacgacgttg
     1081 cgcgggttgc tggtgttggt gcagctcgtg atcgccgcga tgatgaccgc gccatcgggc
     1141 agcaggccgt cggcctcctc ggccctcgcc tgcgcgagct tggcttcgtc ggcgatgccg
     1201 cgctcggcga gcgccgaggt cggcaggcgc ttgtgcgggt tcgagggccc cgccatgttg
     1261 cgcacgaccg tcgacaggtc aaacgtcagc acgcgctcgt actcggcgct tgccagcgca
     1321 tcgccccaga ggccgagcgt cttcgcgtac tgctcgacca gcgccacctg ctccggctcg
     1381 cggccggtca gcttcaggta atcgatggtc tggccgtcga tgtagaacat cgcggcggtc
     1441 gcaccgtact cggggcacat gttcgagatg gtcgcgcgat cgccgatcga caggctctcg
     1501 gcgccttcgc cgaagaactc gacgtaggcc ccgaccacgc gctccttgcg caggaactcg
     1561 gtcagcgcca gcacgatgtc ggtggcggtg atgccgggct ggcgcttgcc ggtgagcctg
     1621 acgccgacga tgtcgggcag gcgcatcatc gacgcgcgtc cgagcatgac ggtctcggct
     1681 tcgagtccgc cgacgccgat ggcgatgacg ccgagcgcat cgacgtgcgg cgtgtggctg
     1741 tcggtgccga cgcaggtgtc cgggaaggcg acgccgtcac ggacctggat caccggcgac
     1801 atcttctcca gattgatctg gtgcatgatg ccgttgccgg ccgggatcac gtcgacgttc
     1861 tggaaggccg tgcgcgtcca gtcgatgaag tggaagcggt cctcgttgcg acgctcctcg
     1921 accgcgcggt tcttgtcgaa ggcctcgggg tcgaagccgc cgtactcgac ggcgagcgag
     1981 tgatcgacga tcagctgcgt cggcaccacc ggattgacct tggccggatc gccgccctga
     2041 tcggcgatcg cgtcgcgcag gccggcgagg tcgacgagcg cggtctggcc gaggatgtcg
     2101 tggcagacga cgcgcgccgg gaaccacggg aagtcgaggt cgcgcttgcg ctcgatcagc
     2161 tggctgagcg aatcgcgcag catcgccggt tcgcagcggc gcacgaggtt ctcggccagc
     2221 acgcgcgagg tgtagggcag cgtcgcccag gccccggcct tgatctcatc gacggcggca
     2281 cgcgcatcga agtaatccag cttcgtgccc ggcagcagtt tgcgataggc agtgttcatg
     2341 gtcacttgcg gtcctcgatc gcgacgaacg gccggtcttc cgggccgatg tagttggcgc
     2401 tcgggcggat gatcttgccg tcctcgcgct gctcgatgac gtgtgcggcc cagccggtgg
     2461 tgcgcgcgat cacgaacagc ggcgtgaaca tctccgtcgg cacgcccatc aggtggtacg
     2521 agacggccga gaaccagtcg aggttcggga acatcttctt ggcgtcccac atgacggttt
     2581 cgagccgatc ggcgatgtcg aacagcttga agttctcggc atcggcggcg agcgtgcgcg
     2641 cgacttcctt gatcaccttg ttgcgcggat cggagatcgt gtagaccggg tgaccgaagc
     2701 cgatgacgac ttccttgcgc tcgacgcgct cgcggatgtc ggcctcggcc tcttccgggt
     2761 tgtcgtagcg ctgctggatc tcgaaggcga cctcgttggc gccgccgtgc ttcggaccgc
     2821 gcagcgcgcc gatcgcgccg gtgatcgccg agtacaggtc ggacgaggtg ccggcgatga
     2881 cgcggccggt gaaggtcgag gcgttgaact cgtgctcggc gtacagcacc agcgaggtgt
     2941 gcatcgagcg cacgtgcagc tcgctcgggc gctcgccgtg cagcaggcgc aggaagtggc
     3001 cgccgatcga ctcgtcgtcg gtctcgacct cgatgcgctt gccgttgtgg ctgtagtggt
     3061 accagtacag cagcatcgag ccgagcgagg cgatcaggcg gtcggcgatg tcgcgcgcac
     3121 cggcggagtt gtgatcgtcc ttctccggca gcacgcagcc gagcgtcgag acggccgtgc
     3181 gcatgacatc catcgggtgc gcggcggccg gaatctgctc cagcaccgcc ttgaccggca
     3241 gcggcaggcc gcgcagcgcc ttgagcttgg ccttgtaggc cttcagctcg ctcgcggtcg
     3301 gcagcttgtg atggatcagc aggtgcgcga tctcctcgaa ctcgcacttg tcggccagat
     3361 cgaggatgtc gtaaccgcgg tagttcaggt cattgccggt gcgcccgacg gtgcagatcg
     3421 cggtgttgcc ggcggcggtg ccggacagcg cgacggactt cttggccttg ggcaggacct
     3481 gcgtggattc ggtcatggct ggggactccg atgtcggttg agcgcggctt tacttggcgt
     3541 ctttgctgaa cagcgcgtcg agcttctgct cgtaggcgtg gtagccgagg tgctcgtaga
     3601 gttcggcgcg ggtctgcatc gtgtcgacga cgccctgctg ggtgccgctg gcacgcagct
     3661 gctgatagac gttcagcgcc gccttcgaag ccgcacggaa ggccgacagc gggtagagca
     3721 cgaggctgac gccgttggcg ccgagctcgt cggtcgtgaa cagcggcgtc gagccgaact
     3781 cggtgatgtt ggcgagcacc ggcacgccga cggcctcggc gaactggcgg tacatgccga
     3841 gatcggtcat cgcctccggg aagatcatgt cggcaccggc ctcgacgcag gcgcaggcac
     3901 ggtcgatcgc cgactgcagg ccctcgacgg cgagcgcgtc ggtacgggcc atgatcacga
     3961 agttgctgtc ggtcttggca tcgaccgcgg ccttgacgcg atcgaccatc tcggcctgcg
     4021 agacgatggc cttgttcggg cggtgaccgc agcgcttctg cgcgacctgg tcctcgatgt
     4081 gcacggcggc gacgccggcg cgggtcagct cgcgcacggt gcgggcgatg ttgaaggcgc
     4141 cgccccagcc ggtgtcgatg tcgacgagca gcggcaggtc ggtgacatcg gtgatgcgcc
     4201 gcgcatcgat cagcacgtct tccatcgtcg tgatgccgag gtccgggatg ccgcaggagc
     4261 aggccgcgac accaccgccg gacaggtaca gggccttgta gcccgagtgt tcggcgagcc
     4321 gggcgaaata ggcgttgacc gccccgacca cctgcaacgg gcgctcctca cgaacggcgg
     4381 cacggaaacg tgcaccggcg gacttcagct cactcatttc gcttgacctc ggatggcagg
     4441 cggcacggat cgcgcgccgc cggatgaaca tcggaaaacg gtcccccgcc cccccggacg
     4501 ggggaccgcc acggcgcgct tgcgcaccgt tccccacggc tgg
//
LOCUS       DAOQIE010000302         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11149_length_4532_cov_8.929864, whole genome shotgun sequence.
ACCESSION   DAOQIE010000302 DAOQIE010000000
VERSION     DAOQIE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11149_length_4532_cov_8.929864"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2175)
                     /gene="relA"
                     /locus_tag="PK144_15415"
     CDS             complement(<1..2175)
                     /gene="relA"
                     /locus_tag="PK144_15415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:NF008124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HNF67165.1"
                     /translation="MVATSHTPSPLYDFDAWIAGLPVRWSPAQVARVREAHALALAAL
                     PGEPDPDTPPPALAVADILAGLRLDHEVVCAALLQPENVAGCLTPGVLAEQFGDSIAR
                     LVEGAEKITVIGDFHQQVHAHGDQLERLRKMLLAMAQDVRVVLIALARRLHRLRTLAG
                     YDPIAQQRIARETLDLYAPLANRLGIARVKWELEDLALRWLAPDTYKELARALDERRV
                     EREAYIEAAMDELRAELEHAGIHAEVTGRVKHIYSIWKKMQRKRLPFEQIFDVRAVRV
                     IVDTVADCYAALGVVHARWNHIRKEFDDYIANPKANGYRSLHTAVFGPDGRTLEIQIR
                     TLEMHQNAELGVAAHWRYKEGASGAQQPSSYEQQIAWLRQMLEARDDGDEDDDRDGED
                     LLERVKAEAFQDRVYVLTPRGEIVELPQGATPLDFAYHVHTEIGHRCRGAKVNGRIVP
                     LSYELKNGEQVDVLTTKSGSPSRDWLSPHLGYLKSTRARNKVRAWFRQLDLEHSIASG
                     RQALDRELHRLGVRSVNLERLAETLEYDQPEELYAAIGRGDLTATHVVSVAQDMILPP
                     PPAETTMPLQRPTITSEPGEIKISGVGKLLTQLARCCKPVPYDAIVGFITQGRGVTIH
                     RADCPNLLDLQSHSRERLIEVSWGDETAPRYNVDVQIDAYDRTGLLRDITQVLANDRI
                     NVIAVNTLSDKQAGTARMNLTVEIGDIGQLARLLDKIAQLPNV"
     gene            complement(2218..3048)
                     /locus_tag="PK144_15420"
     CDS             complement(2218..3048)
                     /locus_tag="PK144_15420"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNF67166.1"
                     /translation="MNARELAANVTELTTLPDVYQQIRALADDPHASLKQFADVVSMD
                     AAISARLLRLVNSPFYGLPGKVDSVLRAINLIGTDELCNLVLASSIGGMFKGVSNAAL
                     DLPAFWWRNVSVAMLARQLGRQNHMPSHERLFIAGILHDVGRLVAFQQVPTLAAEIER
                     TQTDAYDTGRQRAVLGFGYAELGAELLHQWGMPEALVEIVGCQPQPQRALEYGRDAAV
                     LHIAARAVPLIAADPGTPAELETLVAEDLWEESGLDAGQLDTALAAVRERGSEVRGLF
                     "
     gene            3190..>4532
                     /gene="cysG"
                     /locus_tag="PK144_15425"
     CDS             3190..>4532
                     /gene="cysG"
                     /locus_tag="PK144_15425"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNF67167.1"
                     /translation="MDFLPLFLRLTGRPALVVGGGEVAARKVALLLDAGAEVRVVAPE
                     LGTTLAGEYAAGRIRHVARHFAAPDLDGADLVIAATDDVVVNAEVSRLARARSLPVNV
                     VDNPALCSFIMPAIVDRSPVIVALSTGGASPVLARLLRGRLESLIPAAYGKLARLAAD
                     FRSRVQHAVPEARRRAFWEDALQGAVAEDVLAGREAEGRARLEALLEAAGHAPDDAPG
                     EVYLVGGGPGDPELLTFRALRLMQQADVVVYDRLISDGVLALVRRDAERIYVGKARAE
                     HTLPQEEINALLVRLARAGRRVLRLKGGDPFIFGRGGEEIDTLMAEGIPFQVVPGITA
                     ASGCAAYAGIPLTHRDHAQSVTFVTGHPKRDGDLRLNWPALAMPNQTLVFYMGVHGIQ
                     AIRAGLLDHGMPPDTPVAIVERGTQPDMRTHVSTLAEVCALVERTEISPPALLIIG"
BASE COUNT          577 a         1535 c         1680 g          740 t
ORIGIN      
        1 cacgttcggc aactgcgcga tcttgtcgag caggcgcgcg agctggccga tgtcgccgat
       61 ttcgacggtc aggttcatgc gcgcggtgcc ggcctgcttg tcggagagcg tgttgacggc
      121 gatgacgttg atgcggtcgt tggcgagcac ctgcgtgatg tcgcgcagca ggccggtgcg
      181 gtcgtaggcg tcgatctgca catcgacgtt gtagcgcggc gcggtctcgt cgccccagct
      241 cacctcgatc agccgctcgc ggctgtgact ctgcaggtcg agcaggttcg ggcagtcggc
      301 gcggtggatg gtgacgccgc ggccctgggt gatgaagccg acgatggcgt cgtagggcac
      361 cggcttgcag cagcgcgcga gctgggtcag cagcttgccg acgccgctga tcttgatctc
      421 gccgggttcg ctggtgatgg tcggccgctg cagcggcatc gtggtttcgg ccggcggcgg
      481 cggcaggatc atgtcctgcg cgaccgagac gacgtgggtc gcggtcaggt cgccgcggcc
      541 gatcgcggcg tagagctcct cgggctggtc gtattcgagc gtttcggcca ggcgttcgag
      601 gttgacgctg cgcacgccga ggcggtgcag ctcgcgatcg agtgcctgac ggccgctggc
      661 gatgctgtgt tcgaggtcga gctggcggaa ccaggcgcgc accttgttgc gggcgcgcgt
      721 gctcttcaga taaccgaggt gcggtgacag ccagtcgcgg ctcgggctgc cggatttggt
      781 ggtcagcacg tcgacctgct cgccgttctt cagctcgtag ctgagcggca cgatgcggcc
      841 gttgaccttg gcgccgcggc agcggtggcc gatctcggtg tgcacgtggt aggcgaaatc
      901 gagcggcgtc gcgccctggg gcagctcgac gatctcgccg cgcggcgtca gcacgtagac
      961 gcggtcctgg aaggcttcgg ccttcacgcg ctccagcagg tcctcgccgt cgcggtcgtc
     1021 gtcctcgtcg ccgtcgtcgc gggcttcgag catctggcgc agccaggcga tctgctgctc
     1081 gtagctgctc ggctgctgcg cgccgctcgc gccttccttg tagcgccagt gcgcggcgac
     1141 gccgagttcg gcgttctggt gcatctccag cgtgcggatc tggatttcca gcgtgcgccc
     1201 gtccgggccg aacacggcgg tgtgcagcga gcggtagccg ttggccttgg ggttggcgat
     1261 gtagtcgtcg aattccttgc ggatgtggtt ccagcgcgcg tgcacgacgc cgagcgcggc
     1321 gtagcagtcg gcgaccgtgt cgacgatcac gcgcacggcg cgcacgtcga agatctgctc
     1381 gaacggcagg cgcttgcgct gcatcttctt ccagatgctg tagatgtgct tgacgcggcc
     1441 ggtgacctcg gcgtggatgc cggcgtgttc gagttcggcg cgcagctcgt ccatcgccgc
     1501 ctcgatgtag gcctcgcgtt cgacgcggcg ctcatcgagc gcgcgcgcga gttccttgta
     1561 ggtgtccggc gccagccagc gcagcgccag gtcctcgagt tcccacttga cgcgggcgat
     1621 gccgaggcgg ttggcgagcg gggcgtagag atcgagcgtc tcgcgggcga tgcgctgctg
     1681 cgcgatcggg tcgtagccgg cgagcgtgcg cagccggtgc aggcggcggg cgagggcgat
     1741 cagcacgacg cgcacgtcct gcgccatcgc cagcagcatc ttgcgcaggc gttcgagctg
     1801 gtcgccgtgc gcatgcacct gctggtggaa gtcgccgatg acggtgatct tctcggcgcc
     1861 ctcgaccagg cgcgcgatgc tgtcgccgaa ctgctcggcg agcacgccgg gcgtcaggca
     1921 gccggcgacg ttctcgggct gcagcagcgc ggcgcagacg acttcgtgat cgaggcgcag
     1981 gccggcgagg atgtcggcga ccgcgagcgc cggcggcggc gtgtcgggat cgggctcgcc
     2041 gggcagcgcg gcgagcgcca gcgcgtgggc ctcgcgcacg cgcgcaacct gcgccgggct
     2101 ccagcgcacc ggcaggccgg cgatccaggc gtcgaagtcg tacagcggac tcggggtatg
     2161 gctggtcgcg accacgaagg cgtctcctgc ggctgcgggg cggcgatgac cggcgcttca
     2221 gaacagtccg cggacttcgg agccgcgctc gcgcacggcc gcgagggcgg tgtcgagctg
     2281 gccggcatcg aggccgcttt cctcccagag gtcctcggcg accagggttt ccagctcggc
     2341 cggcgtgccg gggtcggccg cgatcagcgg cacggcacgg gcggcgatgt gcagcacggc
     2401 ggcatcacgg ccgtattcga gcgcgcgctg cggctgcggc tggcagccga cgatctcgac
     2461 cagcgcctcc ggcatgcccc actggtgcag cagttcggcg ccgagttcgg cgtagccgaa
     2521 gccgagcacg gcgcgctggc ggccggtgtc gtaggcgtcg gtctgcgtgc gctcgatctc
     2581 ggcggcgagc gtcggcacct gctggaaggc cacgagccgg ccgacatcgt gcaggatgcc
     2641 ggcgatgaac aggcgctcgt gggacggcat gtgattctgc cgcccgagct ggcgcgcgag
     2701 catcgcgacg ctgacgttgc gccaccagaa cgccggcagg tcgagcgcgg cattgctgac
     2761 gcccttgaac atgccgccga tcgagctggc gagcacgagg ttgcagagct catcggtgcc
     2821 gatcaggttg atcgcgcgca gcacgctgtc gaccttgccg ggcaggccgt agaacggact
     2881 gttgaccagg cgcagcagcc gcgccgaaat cgcggcatcc atgctgacca cgtcggcgaa
     2941 ctgcttgagc gaggcatgcg gatcatcggc cagtgcccgg atctgctggt acacatcggg
     3001 cagcgtggtc agttcggtga cgttggcggc gagctcgcgc gcgttcatct tcggcgacct
     3061 tcggcgtgac ggggggcggt atctaagcac accataccgg cccctgacag tcgcgtgcgc
     3121 gcccggcagc attcgttgca cggtgctaca agtcgcgtcc ttcctgcccc ccagccccga
     3181 ggtgtcgtcg tggatttcct gcccctgttc ctgcgcctga ccggccgccc ggcgctggtc
     3241 gtcggcggcg gcgaggtggc cgcgcgcaag gtggcgctgc tgctcgatgc cggtgccgag
     3301 gtgcgcgtcg tcgcaccgga actcggcacg acgctcgccg gcgagtacgc cgccggccgc
     3361 atccgccacg tcgcacgcca tttcgccgcg cccgatctcg acggcgccga tctggtcatc
     3421 gccgccaccg acgacgtggt cgtcaacgcc gaggtctcgc ggctggcgcg cgcgcgttcg
     3481 ctgccggtca acgtcgtcga caacccggcg ctctgcagct tcatcatgcc ggcgatcgtc
     3541 gatcgctcgc cggtgatcgt cgcgctgtcg accggtggtg cctcgccggt gctggcgcgg
     3601 ctgctgcgcg ggcggctcga atcgctgatc ccggccgcct acgggaagct cgcgcgcctc
     3661 gccgccgatt tccgcagccg cgtgcagcac gccgtgcccg aggcccgccg ccgcgcgttc
     3721 tgggaggacg cgctgcaggg cgcggtcgcc gaggatgtgc tcgccgggcg cgaggccgag
     3781 ggtcgcgcgc ggctcgaagc cctgctcgaa gccgccggcc atgctccgga cgatgcgccc
     3841 ggcgaggtct atctggtcgg cggcggcccc ggcgatccgg agctgctgac cttccgcgcg
     3901 ctgcgcctga tgcagcaggc cgatgtcgtc gtctacgacc ggctgatttc cgacggcgtg
     3961 ctggcgctgg tgcgccgcga tgccgagcgc atctacgtcg gcaaggcgcg cgccgagcac
     4021 acgctgccgc aggaagaaat caacgcgctg ctggtgcgcc tcgcccgcgc aggccggcgc
     4081 gtgctgcgcc tcaagggcgg cgatccgttc atcttcggcc gcggcggcga ggagatcgac
     4141 acgctgatgg ccgagggcat cccgttccag gtcgtgcccg gcatcaccgc ggcgagcggc
     4201 tgcgcggcct acgccggcat tccgctgacg caccgcgatc acgcgcagtc ggtgaccttc
     4261 gtcaccggcc acccgaagcg cgacggcgac ctgcggctca actggccggc gctggcgatg
     4321 ccgaaccaga cgctggtgtt ctacatgggc gtgcacggca tccaggccat ccgcgccggc
     4381 ctgctcgatc acggcatgcc acccgacacg ccggtcgcga tcgtcgagcg cggcacgcag
     4441 ccggacatgc gcacgcacgt ctcgacgctc gccgaggtct gcgcgctggt cgagcgcacc
     4501 gagatcagcc cgccggcgct gctgatcatc gg
//
LOCUS       DAOQIE010000303         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11151_length_4532_cov_6.839848, whole genome shotgun sequence.
ACCESSION   DAOQIE010000303 DAOQIE010000000
VERSION     DAOQIE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11151_length_4532_cov_6.839848"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK144_15430"
     CDS             <1..310
                     /locus_tag="PK144_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67168.1"
                     /translation="LLGPAGSALARTLASVSGEDFDLAATRVWGARECLKKAGLAVNT
                     PLTLAEAAGDAVRFAAGGRALVTIACRPDGVAAVAALLAEEDAPAAAGARSPGRLAGS
                     "
     gene            334..1347
                     /locus_tag="PK144_15435"
     CDS             334..1347
                     /locus_tag="PK144_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF67169.1"
                     /translation="MKLTRRQLLSTAAVLAVPGALALLRFRPWESAQAQEALTVGYLP
                     VTCHLTCPVTDYASRSSRFARFDSQRYLDFPTMVEAIKSGRLQASFMVVPLAMKLREQ
                     GVPVKLVYLGHREGSTLMVRAANPATDLAALRGTSFAIPTRYSNQNLVLHRLMAQQGL
                     TEADLKIVELPPPDMPGALAAGAIEGYFVGEPMAAKAELAGTGRVLYHAHAIWPGFIS
                     CGLVVHEELIERKPALIADLVRGIAQSGQWIEHHRPEAAAIAAPYYRQEERLLRHVLT
                     DPGRVSYVNLTPQDADLQAIHTLAVQLGILTQPLRMSELIDRRFVPGRIDAIELAPPE
                     RPA"
     gene            1344..2171
                     /locus_tag="PK144_15440"
     CDS             1344..2171
                     /locus_tag="PK144_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67170.1"
                     /translation="MSTPGAAAARPAAIAGRAPLRRRLAGVPGLPAVAVALLALVLWS
                     ALARWGGWADYVLPSPAQVLAGLGEDLASGRLATDVVASLYRVMLGYGLACALGVPCG
                     LVLGHAPLARAALLPGVNFFRALSPLAWIPFAILWFGIGDVPVVFLIFMATFFPLVLA
                     VMGAVANVPPIWLRVGREYGLRGPRLLAHVTLPAILPQLITALRVTVGLAWLVVVAAE
                     MVAGRDGLGFAIWDARNGLRADLLVARMAAIGTIGVVLDALLGRLTRLPGVRWGHGG"
     gene            2161..2856
                     /locus_tag="PK144_15445"
     CDS             2161..2856
                     /locus_tag="PK144_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF67171.1"
                     /translation="MGAEVCALRGIVQGYAGLPVLDELWLTVLPGEFVAVVGPSGCGK
                     STLLNLLAGFAEPQAGELHRPVATRMVHQQDGLLPWLTAAGNIALGLGSKDEADPRLS
                     GWLRLAGLEAFAGYYPHQLSGGMRRRVELARVLASPAGLLLLDEPFAALDWLTRRRIH
                     AELMRLLALQPRAVVLVTHDIEEAVTLADRVIVLSPRPARVRCELPLDAPRPRPLAAA
                     LPAMQRILAELET"
     gene            2869..3306
                     /locus_tag="PK144_15450"
     CDS             2869..3306
                     /locus_tag="PK144_15450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF67172.1"
                     /translation="MPASPAFEMRHVVTFEETNLVGNVYYANHVRWQGRCRELFLHAH
                     APQLLDEVARGLVLVTTRVACEYFDELYAFDEIIVRMRAGEIGQGRVTMLFEYWRVRE
                     GAESLVAQGEQQIACLRCAEDGRHVPTGLPEVLVRALEPYRQH"
     gene            complement(3377..4003)
                     /locus_tag="PK144_15455"
     CDS             complement(3377..4003)
                     /locus_tag="PK144_15455"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HNF67173.1"
                     /translation="MALALLLAGCVASRPELFPPPAGAPTVAIRLVSHGWHTGLVLPR
                     AALTGRLPALAGQFVQAEWLEIGWGDLGFYTAPDQQITSGLTLQALFASRGSVLHVVG
                     LNGPPEQAFPHSDVQPVVLGEAGFAALADGIEASFAASPAVALGPGLYGDSRFYAARG
                     HYWALHTCNTWTAERLLEAGCPVTPFWALGAGNTMWQARRHCAVNAAD"
     gene            complement(4065..>4532)
                     /locus_tag="PK144_15460"
     CDS             complement(4065..>4532)
                     /locus_tag="PK144_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNF67174.1"
                     /translation="RKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVA
                     AGLAEKCEIQVSYAIGVAQPTSISVTTFGTGKVSDARIVELVREYFDLRPYAITKMLD
                     LLHPMYQATAAYGHFGRTPYEMTWSDKELTRTFTAFPWEKTDRAAALRDAAGL"
BASE COUNT          561 a         1607 c         1701 g          663 t
ORIGIN      
        1 cctgctcggc ccggccggca gcgcgctggc gcgcacgctg gcctcggtga gcggcgagga
       61 cttcgacctg gctgccacgc gcgtctgggg cgcgcgcgaa tgcctgaaga aggccgggct
      121 ggccgtcaac acgccgctga cgctcgccga ggccgccggc gatgccgtcc ggttcgcggc
      181 cgggggccgg gcgctggtga cgatcgcctg ccggcccgac ggcgtggcag cggtcgcggc
      241 gctgctggcc gaagaggacg cgccggccgc tgccggggcg cgcagccccg ggcggctggc
      301 cggctcctga gtcaacgacg ggaggatctg ccgatgaaac tgacccgcag acaactgctg
      361 agcactgccg ccgtgctggc ggtgccgggg gcgctggcgc tgttgcgctt ccggccctgg
      421 gagagcgcgc aggcgcagga ggctctgacc gtcggctacc tgccggtgac ctgccacctg
      481 acctgtccgg tcaccgacta cgcgagccgc agcagccgct tcgcacgctt cgattcgcag
      541 cgctacctgg acttcccgac catggtcgag gcgatcaagt ccgggcggct gcaggcgagc
      601 ttcatggtcg tgccgctggc gatgaagctg cgcgagcagg gcgtgccggt gaagctcgtc
      661 tatctcgggc atcgcgaggg ttcgacgctc atggtgcggg ccgcgaaccc ggcgaccgac
      721 ctcgcggcgc tgcgcggcac gagcttcgcg atcccgaccc gctacagcaa ccagaacctc
      781 gtgctgcacc ggctgatggc gcagcagggc ctgaccgagg ctgacctgaa gatcgtcgag
      841 ctgccgccgc cggacatgcc cggggcgctc gccgccggcg cgatcgaggg ctacttcgtc
      901 ggcgagccga tggccgcgaa ggccgaactc gccggcaccg gccgcgtgct gtaccacgcg
      961 cacgcgatct ggccgggctt catctcctgc ggcctggtcg tgcacgagga actgatcgag
     1021 cgcaagccgg cactgatcgc cgatctggtg cgcggcatcg cgcagagcgg ccagtggatc
     1081 gagcatcacc ggcccgaggc cgccgcgatc gccgccccgt actaccggca ggaggaaaga
     1141 ctgctgcgcc acgtgctgac cgatcccggc cgggtcagct acgtgaacct gacgccgcag
     1201 gatgcggatc tgcaggccat ccacacgctc gccgtgcagc tcggcatcct gacgcagccg
     1261 ctgcgcatga gcgagctgat cgaccggcgc ttcgtgcccg gacgcatcga cgcgatcgaa
     1321 ctcgccccgc cagagcgtcc ggcatgagca cacccggagc cgccgccgcc cgcccggcgg
     1381 ccatcgccgg gcgggcgccg ctgcgccggc ggctcgccgg ggtgcccggc ctgccggcgg
     1441 tcgcggtcgc gctgctcgcg ctcgtgctct ggtcggcgct ggcgcgctgg ggcggctggg
     1501 ccgactacgt gctgccgtcg ccggcgcagg tgctggcggg gctcggcgag gatctggcct
     1561 ccgggcggct cgcgaccgac gtggtcgcgt cgctgtaccg cgtgatgctc ggctacgggc
     1621 tcgcctgcgc actcggcgtg ccctgcggac tcgtgctcgg gcatgcgccg ctcgcgcggg
     1681 ccgcgctgct gccgggcgtg aacttcttcc gggcgctgtc gccgctcgcg tggattccgt
     1741 tcgcgatcct gtggttcggc atcggcgacg tgccggtcgt gttcctgatc ttcatggcga
     1801 ccttcttccc gctggtgctc gcggtgatgg gcgcggtcgc caacgtgccg ccgatctggc
     1861 tgcgcgtcgg gcgtgaatac ggcctgcgcg gaccgcggct gctcgcgcac gtgacgctgc
     1921 cggcgatcct gccgcagctg atcacggcgc tgcgcgtgac ggtcgggctc gcgtggctgg
     1981 tcgtggtcgc cgccgagatg gtcgccgggc gcgacggcct cggcttcgcg atctgggatg
     2041 cacgcaacgg cctgcgcgcc gacctgctgg tggcgcgcat ggcggcgatc ggcacgatcg
     2101 gcgtcgtgct cgatgcgctg ctcgggcgcc tgacgcggct gccgggcgtg aggtggggtc
     2161 atgggggctg aggtctgcgc actgcgcggc atcgtgcagg gctatgccgg cctgccggtg
     2221 ctcgacgagc tctggctgac ggtgcttccg ggcgagttcg tcgccgtggt cgggccttcg
     2281 ggctgcggca agagcacgct gctgaacctg ctcgccggtt tcgccgaacc gcaggccggc
     2341 gagctgcacc ggccggtggc cacgcgcatg gtccaccagc aggacggcct gctgccgtgg
     2401 ctgaccgcgg ccggcaacat cgcgctcggg ctcggcagca aggacgaggc cgatccgcgc
     2461 ctgtccggct ggctgcgcct cgccggcctc gaagccttcg ccggctacta cccgcaccag
     2521 ctctccggcg gcatgcgccg gcgcgtcgaa ctcgcgcgcg tgctggcgag cccggccggc
     2581 ctgctgctgc tcgacgagcc cttcgccgcg ctcgactggc tgacgcgccg gcgcatccac
     2641 gccgagctga tgcggctgct ggcgctgcag ccgcgcgccg tcgtgctggt cacgcacgac
     2701 atcgaggagg ccgtgacgct cgccgatcgc gtgatcgtgc tgtcgccgcg cccggcacgt
     2761 gtacgctgcg agctgccgct cgatgcgccg cgcccgcggc cgctcgcggc ggcgctgccg
     2821 gcgatgcagc gcatcctcgc cgaactcgaa acctgaggag ccttgccgat gcccgcgtcc
     2881 cccgccttcg agatgcgcca cgtcgtcacc ttcgaggaga ccaatctggt cggcaacgtc
     2941 tactacgcca accacgtgcg ctggcagggg cgctgccgcg agctgttcct gcacgcgcac
     3001 gccccgcaac tgctcgacga ggtcgcgcgc ggcctggtgc tggtcacgac gcgcgtcgcc
     3061 tgcgagtact tcgacgagct gtacgccttc gacgagatca tcgtgcgcat gcgcgccggg
     3121 gagatcggtc aggggcgcgt gacgatgctc ttcgagtact ggcgcgtgcg cgagggcgcc
     3181 gaatccctgg ttgcgcaggg tgagcagcag atcgcctgcc tgcgctgcgc cgaagacggc
     3241 cggcacgtgc cgaccgggct gccggaggtg ctggtgcggg cgctggaacc gtatcgccag
     3301 cactgaggcc ggcttgcgcg aacggcgcgg ctccggttgc gtggggcgtg gcttgccgtg
     3361 cggccggggg cgccgttcag tccgcggcgt tcacggcgca gtgacgccgc gcctgccaca
     3421 tcgtgttgcc ggcgccgagc gcccagaacg gcgtgaccgg gcagccggct tcgagcaggc
     3481 gttcggccgt ccaggtgttg caggtgtgca gcgcccagta atggccgcgc gctgcgtaga
     3541 agcggctgtc gccgtacagg ccggggccga gcgccacggc cggcgaggcg gcgaagctcg
     3601 cctcgatgcc atcggcgagc gccgcgaagc cggcctcgcc gagcacgacc ggctgcacgt
     3661 cggaatgcgg gaaggcctgc tccggcgggc cgttgaggcc gacgacgtgc agcaccgagc
     3721 cgcgcgacgc gaacagcgcc tgcagcgtca ggcccgaggt gatctgctga tccggcgccg
     3781 tgtagaagcc gagatcgccc cagccgatct ccagccattc ggcctgcacg aactgcccgg
     3841 cgagcgccgg cagccggccg gtgagcgccg cgcgcggcag cacgaggccg gtgtgccagc
     3901 cgtggctgac gaggcggatc gccacggtcg gcgcgccggc gggcggcggg aatagctccg
     3961 ggcgcgaggc gacgcagccg gcgagcagca gtgccagcgc caccggccag ctgcagaaaa
     4021 gaccggggcc gcttgcgcgg ccccggtgtg gtatcggcgg aggcttacag cccggcggca
     4081 tcccgcagcg ccgcggcgcg atcggtcttc tcccacggga aggccgtgaa ggtccgcgtg
     4141 agctccttgt cgctccaggt catctcgtac ggcgtgcgcc cgaagtggcc gtaggcggcg
     4201 gtggcctgat acatcgggtg cagcaggtcg agcatcttcg tgatcgcgta cgggcgcagg
     4261 tcgaagtact cgcgcacgag ctcgacgatg cgcgcatcgc tgaccttgcc ggtgccgaag
     4321 gtcgtcaccg agatcgaggt cggctgcgcg acgccgatcg cgtagctgac ctggatctcg
     4381 cacttctcgg cgaggccggc ggcgacgatg ttcttcgcga cgtagcgccc ggcgtaggcg
     4441 gccgagcggt cgaccttgga cgggtccttg cccgagaacg cgccgccgcc gtggcgggcc
     4501 atgccgccgt aggtgtcgac gatgatcttg cg
//
LOCUS       DAOQIE010000304         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11250_length_4508_cov_6.734561, whole genome shotgun sequence.
ACCESSION   DAOQIE010000304 DAOQIE010000000
VERSION     DAOQIE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11250_length_4508_cov_6.734561"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PK144_15465"
     CDS             <1..170
                     /locus_tag="PK144_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011898536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF67175.1"
                     /translation="FDLSLRAGRITHIAQVSNVKIVHHGGHAARKGWHHIRMFVRSGI
                     TFFNTHGWKLW"
     gene            167..1114
                     /locus_tag="PK144_15470"
     CDS             167..1114
                     /locus_tag="PK144_15470"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF67176.1"
                     /translation="MSVLVTGATGFIGRRLVGELRAQSCTVRIAVRRLLPALTEAVLA
                     PLDDPAALERACAGMDTVYHCAGHAHAWSGQDAALHRQVNHAGTCALATAAGRAGVRR
                     FVFLSSVKAMGEPGEVCADEDWPLPPLTPYGQAKRAAEDAVLEAGARYGMHVVNLRLT
                     MVYGPGGRGNLERMAALVRRGWFPPLPETGNRRSLVYVDDVVSAMRLVAHVPAAAGRT
                     WIVADEAAYSGRELYVALRAALGLAPRRHAVPAGGLATVAAIGDRLERLLGRRLPFDS
                     EVLDKLLGSAWYSSARLRGELGWRPQVPLVEGLQRMCRA"
     gene            1233..2249
                     /locus_tag="PK144_15475"
     CDS             1233..2249
                     /locus_tag="PK144_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF67177.1"
                     /translation="MILLAFLVAAFVSALLTRRFCDPDSSLHLLDLPNARSLHTRPTP
                     RSGGIALLAGVGAALPLLAVGGWLGPAAACVLPAAVPVAVVSFLDDRSGVGVRWRFAV
                     HLAAALGLIVLVGLPAGLSLPGAVWQPARPLLTVLTVLFCVWMLNLYNFMDGMDGFAG
                     GMTVCGYATLALLGMAAGDAAYAVLAALPAVAALGFLAFNFPPARIFMGDTGAATLGF
                     LAAGLILYAERSGLFPFWAGLLAFAPFIVDATVTLLRRLLRGERIWEAHRSHYYQRLV
                     RLGWGHRRTVLAEYALMLVCAAAALAGPRLPAAGQALLLAATALLFVTLAAGVARLER
                     SAAP"
     gene            2246..>2583
                     /locus_tag="PK144_15480"
     CDS             2246..>2583
                     /locus_tag="PK144_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNF67178.1"
                     /translation="MKIWLRIRHPLAALAHDLLMTPLAWFGAQWLRFNFDAIPTPFLQ
                     AAQQLLPLVLFVHAACYVRFGLYRGVWRFASMPDLVRICKAVVTGTLICWALVALAAQ
                     IGLLPPVPVPR"
     assembly_gap    2584..2683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2684..3982
                     /locus_tag="PK144_15485"
     CDS             <2684..3982
                     /locus_tag="PK144_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNF67179.1"
                     /translation="RTQDVAAIADRHAIDMIFVAMPSLGGERMARVLRLCEASGRPVR
                     VVPRLDELLSGRAQIRELREVSLEDLLGREEVTLDWETIGRCVAGRTVLVSGGGGSIG
                     SELCRQIARLGPARLLIVDAGEFNLYRIELELRRDWPEVSLQVFLLDVTDAIGVGQVF
                     ATQRPELVFHAAAYKHVPLLEDQPSTAIGNNVFGTMTLAQAADCHGADAFVLISTDKA
                     VNPTNVMGASKRLAEMYCQNLAARSHTHFITVRFGNVLGSAGSVVPLFREQIARGGPV
                     TVTHPEIERYFMTIPEAAQLILQAAAVGRGGEIFVLDMGAPIRIATLAEQMIQLSGKR
                     PHQDIRIEFTGLRPGEKLYEELFHEREQLQPTVYPKLLLAQRRTLDWGAFNRALEALR
                     ADCRDRRDAEVLAAMHVLVPEYRASGSEGGQGAPVQATGT"
     gene            complement(3990..>4508)
                     /gene="ccsA"
                     /locus_tag="PK144_15490"
     CDS             complement(3990..>4508)
                     /gene="ccsA"
                     /locus_tag="PK144_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF67180.1"
                     /translation="VLLPFTATTVVLALAFPATRLVTEAAQWRLELHILISITAYALL
                     ALAAVQSLLLAVQERRLRTRRLGGISRGLPPLVTMESLLFQMIGAGFGALTLSLLSGF
                     FFLDNLFAQHLVHKTVLSIAAWLVFATLLWGRRRYGWRGRTAIRWTLSGFAVLVLAYF
                     GSKLVLELVLRR"
BASE COUNT          598 a         1475 c         1598 g          737 t
ORIGIN      
        1 acttcgatct gagcctgcgc gctggcagaa tcacgcacat cgctcaagtg tccaatgtga
       61 agatcgtcca tcacggcggc catgccgcgc gcaagggctg gcatcacatc cggatgttcg
      121 tgcgctcggg catcaccttc ttcaacaccc atggctggaa gctctggtga gcgtgctggt
      181 gaccggcgcc accggtttca tcggcagacg gctggtcggg gaattgcggg cgcagtcctg
      241 caccgtgcgc attgccgtgc gccggctgct gccggcgctg accgaagcgg tgctggcgcc
      301 gctcgatgac ccggcagcgc tggagcgcgc ttgtgccggc atggacacgg tgtaccactg
      361 cgccggtcat gcacatgcct ggtcaggaca ggatgcggcg ctgcaccggc aggtgaatca
      421 tgcaggcacc tgcgcgctcg ctacggcggc ggggcgcgcc ggcgtgcgcc gcttcgtgtt
      481 cctgtccagc gtcaaggcca tgggcgaacc cggcgaggtc tgcgccgacg aggactggcc
      541 gctgccgccg ctgaccccgt acggtcaggc caagcgtgct gccgaagatg cggtgctgga
      601 ggccggagca cgctacggca tgcacgtcgt gaacctgcgg ctgacgatgg tctacgggcc
      661 gggcggacgc ggcaacctgg agcgcatggc ggcgctggtg cggcgcggat ggtttccgcc
      721 gctgccggaa accggcaacc ggcgctcgct ggtgtacgtc gatgacgtgg tatcggcaat
      781 gcggctggtt gcgcatgtgc cggctgctgc cggtcgtacc tggatcgtcg ccgatgaggc
      841 cgcgtattcg gggcgcgagc tgtatgtggc gctgcgcgcg gcgctggggc tcgcgccgcg
      901 ccggcatgcc gtgccggctg gcgggctggc aacggtggcg gcgatcgggg accggctgga
      961 gcgactgctc ggccggcggc tgccgttcga ttccgaggtg ctcgacaagc tgctcggttc
     1021 ggcctggtac tcctcggccc ggctgcgcgg ggagctgggc tggcggccac aggtgccgct
     1081 cgtcgaaggc ctgcagcgga tgtgccgggc ctgagccgga tgcgtcgtcg caagcagcga
     1141 ttgttgcaga tgcacaaagc cgacacgcta aactgccacg cttgccgggc cgtggcgctg
     1201 tgcctgcctg gctccggaca ggtactcagg cgttgatcct ccttgcattc ctggtcgcag
     1261 ccttcgtgtc ggcgctgctg acccggcgtt tctgcgatcc cgattcgagc ctgcatctgc
     1321 tcgatctgcc gaacgcccgt tcgctgcaca cacgcccgac gccgcgcagc ggcggcatcg
     1381 cgctgctcgc cggcgtcggg gcggcactgc cgctgctcgc cgtcggcggc tggctcgggc
     1441 cggcggcggc ctgcgtgctg ccggcggcgg tgccggtggc ggtcgtgtcg tttctggatg
     1501 atcgcagcgg tgtgggcgtg cggtggcgct tcgccgtgca tctggcggca gcactggggc
     1561 tgatcgtgct ggtcgggctg ccggcaggcc tgagcctgcc cggtgctgtc tggcagccgg
     1621 cccggccgtt gctgaccgtg ctgacggtgc tgttctgcgt ctggatgctg aatctctaca
     1681 acttcatgga cggcatggac ggcttcgcag gtggcatgac cgtgtgcggt tacgcgacgc
     1741 tggcgctgct cggcatggct gccggggacg ctgcgtacgc tgtcctggcg gccttgccgg
     1801 ccgtggcggc gcttggtttt ctcgcgttca acttcccgcc ggcgcgcatc ttcatggggg
     1861 ataccggagc tgcgaccctc ggcttcctgg cggccggcct gatcctgtac gccgaacgca
     1921 gcgggctgtt cccgttctgg gccgggctgc tggcgtttgc gccgttcatc gtcgatgcca
     1981 cggtgacgct gctgcgccgg ctgctgcgcg gcgagcgcat ctgggaggca caccgcagcc
     2041 attattacca gcggctggtg cgcctcggct gggggcaccg gcgcacggtg ctggccgagt
     2101 acgcgctgat gctggtctgc gccgcggcgg cgctcgccgg tccccggctg ccggccgccg
     2161 gtcaggcact gctgctcgcc gcaacggcat tgctgttcgt gacgctcgcg gccggcgtcg
     2221 cccggctgga gcggagtgcg gccccatgaa gatctggctg cgcatccgcc atccgctggc
     2281 ggcactcgcc catgatctgc tgatgacgcc gcttgcctgg ttcggcgcgc agtggctgcg
     2341 attcaatttc gacgccatcc cgacgccgtt cctgcaggcc gcccagcaac tgctgccgct
     2401 cgtactgttc gtgcatgccg cctgttacgt gcgtttcggg ctgtatcgcg gggtctggcg
     2461 cttcgcgtcg atgccggatc tggtgcgtat ctgcaaggcg gtcgtgaccg ggaccctgat
     2521 ctgctgggca ctggtggcgc tggccgcaca gatcggcctg ctgccaccgg tgccggtgcc
     2581 gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcacgc aggacgtggc
     2701 ggccatcgcc gatcggcacg cgatcgacat gatcttcgtg gccatgccct cgctcggcgg
     2761 cgaacgcatg gcgcgcgtgc tccggctctg cgaggcgagc ggccggccgg tgcgtgtcgt
     2821 gcctcggctc gatgagctgc tgagcgggcg ggcgcagatt cgcgagctgc gcgaggtctc
     2881 gctcgaagac ctgctcgggc gcgaggaggt cacgctcgac tgggagacga tcggacgctg
     2941 cgtggccggg cgcacggtgc tggtcagtgg cggcggcggc tcgatcggct ccgagctctg
     3001 ccgtcagatc gcccggctcg ggccggcgcg gctgctgatc gtcgatgccg gcgagttcaa
     3061 cctgtaccgc atcgaactcg aactgcgccg cgactggccc gaagtttcgc tgcaggtgtt
     3121 cctgctcgat gtcacggatg ccatcggtgt cgggcaggtg ttcgccacgc agcggcccga
     3181 gctggtgttc catgccgccg cctacaagca cgtgccgctg ctcgaagacc agccttcgac
     3241 ggcgatcggc aacaacgtgt tcggcacgat gacgctggcg caggccgccg attgccatgg
     3301 cgccgacgcc ttcgtgctga tctcgaccga caaggcggtc aatccgacca acgtcatggg
     3361 ggcgagcaag cggctggccg agatgtactg ccagaacctc gccgcccgca gccacacgca
     3421 cttcatcacc gtgcgcttcg gcaacgtgct cggctcggcc ggcagcgtcg tgccgctgtt
     3481 tcgcgagcag atcgcgcgcg gcgggccggt gacggtcacg catcccgaga tcgagcgcta
     3541 cttcatgacc attcccgagg ccgcgcagct gatcctgcag gcagcggcgg tcgggcgcgg
     3601 cggcgagatc ttcgtgctcg acatgggcgc accgatccgc atcgcgacgc tggccgagca
     3661 gatgatccag ctctcgggca agcgcccgca tcaggacatc cgcatcgagt tcaccggcct
     3721 gcgcccgggc gagaagctct atgaggagct gttccacgaa cgcgagcaac tgcagccgac
     3781 cgtctacccg aagctgctgc tcgcgcagcg ccggacactg gactgggggg cgttcaaccg
     3841 ggcgctggaa gccctgcggg ccgactgcag ggatcgcagg gatgccgagg tgctggcggc
     3901 gatgcacgtg ctggtgccgg aataccgggc ctccggcagc gagggggggc agggtgctcc
     3961 ggtgcaggcc accggcacct gagcagcgat cagcgcctga gcaccagttc gagcaccagc
     4021 ttgctgccga agtacgcgag caccagcacc gcaaagccgc tcagcgtcca gcggatcgcg
     4081 gtgcgtccgc gccagccgta ccggcggcgg ccccacagca gcgtggcgaa caccagccag
     4141 gccgcgatcg acagcacggt cttgtgcacg aggtgctgcg cgaagaggtt gtcgagaaag
     4201 aagaaccccg acagcagcga cagcgtcagc gccccgaaac cggcaccgat catctggaac
     4261 agcagcgact ccatcgtcac cagcggcggc agtccgcgcg agatgccgcc cagacggcgc
     4321 gtgcgcaggc gccgttcctg caccgcgagc agcagcgact gcacggcggc gagcgcgagc
     4381 agcgcgtagg cggtgatcga gatcaggatg tgcagttcca ggcgccactg cgcggcctcg
     4441 gtgacgagtc gcgtcgccgg aaacgccagc gccagcacga cggtggtcgc cgtgaacggc
     4501 agcagcac
//
LOCUS       DAOQIE010000305         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11282_length_4501_cov_7.404184, whole genome shotgun sequence.
ACCESSION   DAOQIE010000305 DAOQIE010000000
VERSION     DAOQIE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11282_length_4501_cov_7.404184"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK144_15495"
     CDS             complement(<1..346)
                     /locus_tag="PK144_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67181.1"
                     /translation="MTAARTGLCVNVGNCPSANARKVVPVAPGQDFVCPDCGKSLVVY
                     QPAKKGAQALIIAAAAGVVLALGGVGWWLYRADAEHEATAAAQQAEAARQAEAQRQAA
                     AAEAKRLEAERQA"
     gene            complement(347..3364)
                     /locus_tag="PK144_15500"
     CDS             complement(347..3364)
                     /locus_tag="PK144_15500"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HNF67182.1"
                     /translation="MNHVIVGLGGTGGKVIRALRKLIFQEFRSEEPEGVPLGWLYVDS
                     SDELMAPDDASWKTLGVSVQLPAASQLLITDANLAARLDDLDSYPGIKPWIGAREQWR
                     DILNSIVGVTLGGQKRRLGRFLFACKADKYREQLQTQVRRVQAGGSSETTFHVLCGLA
                     GGTGSGSLIDALVQLRELFPDAQRYRVLVYALLPDPYPPPNWDTGNYHANGYAALLEL
                     NALSAGAWQPHDVLGRKGRLALVDPFNGCYVFGNENEHGVTVDVQQELPGIVAEFLYQ
                     KIIRARHGGWPTLARMENAENGDGSPETAPASRRGERARRFLTFGIKRVAIPEEEIAE
                     FLGYGFARQALLALAHEHWQEALGYVEEPRPFDAGAFVRQKDVQQRWALDDDHLLMNV
                     PILAGDGQAPKWQTLTLEWETAIPHFKSLVRELDRTIWLDELAKYCQKRWDEDFRDLG
                     VTGFYETKLKARKDIAREIANRIERELANDWRIGARGLDACTRLVAAVIELLDERHAQ
                     LGERIARARAGEDEAQTRLNQNARDWAGMGVFAKLTGTPASLLDAHAVYLQELYVNRT
                     RGVALRAQQQLLPELIAALAELKTAIERAAGSLRTALERFGQRLEQRLADGATATGSD
                     LRRHLIRYYDPARVREVAHRLGLDAEVQRGQAAGARAALFGRFGETLRFADFNSRMPL
                     ATLLDTLEKTGEEAARLAHASLIRTPAERVLGVSIIERLAERFADDAAARRAWVGELV
                     RQAGAFTAFEPLEMHRSAPGIPAGTPSALAKLTVIVPKAPAHADFVAALKSDFRGARS
                     GDVEVIDADGGDNEIVLVSIVNLFPLRYLKPVAQLRERYERRLNHSGDPARARLELHT
                     EGDGAQLPPLYVASPEDIRREGLPYVLLARALGVLREAENPATGEPEVLLLGKDADGF
                     DNPPLALGRTLTESWAALDLLKLDALRAQVVQLLADPGWRHRERREQLLETVRACVDA
                     VRLERGDDRQDAQYLRFLDAGKQAARLLKGEER"
     gene            complement(3467..4345)
                     /locus_tag="PK144_15505"
     CDS             complement(3467..4345)
                     /locus_tag="PK144_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNF67183.1"
                     /translation="MRYGDDDRESGNIEDRRGESFGGGGGFGGGGLRIGGGIGTVVLV
                     LVALYFGVDPSVILGGGSPAPVQHPPGPAQTRAHDDEARFVGRVLADTESVWNAQLPA
                     QTGQAYVEPKLVLFSGATQTACGIGQAAMGPFYCPPDRKVYIDLSFYDDLRQRFRAPG
                     DFAQAYVVAHEIGHHIQNLLGISGRVHAARERMSEADGNRLSVRLELQADCLAGVWAR
                     HAEQTKHRLEPGDVEEALTAAAAIGDDRLQRQARGTVVPDSFTHGSSQQRVRWFRAGL
                     DSGRLDACDTFGAARL"
BASE COUNT          599 a         1622 c         1621 g          659 t
ORIGIN      
        1 gcgcctgacg ctcggcctcc agacgcttcg cttcggcggc agcggcctga cgctgggcct
       61 cggcctgacg ggcggcctcg gcctgctgcg cggcggccgt ggcctcgtgt tcggcatcgg
      121 cgcggtacag ccaccagccg acgccaccga gcgcgagcac cacgccggcc gcggccgcga
      181 tgatcaaggc ctgcgcgccc ttcttcgccg gctggtagac gaccagcgac ttgccgcagt
      241 ccgggcagac gaagtcctgc cccggcgcca ccggcacgac cttcctggcg ttggcggacg
      301 ggcagttgcc gacgttgaca cagagtccgg tgcgtgcggc cgtcattcag cgttcctccc
      361 ccttcagcag acgcgcggcc tgcttgccgg cgtccagaaa ccgcaggtac tgcgcgtcct
      421 gccggtcgtc gccgcgttcg agacgcacgg catcgacgca ggcgcgcacc gtttccagca
      481 attgttcacg ccgttcgcga tggcgccacc cggggtcggc gagcagctgc acgacctgcg
      541 cgcgcagcgc atcgagcttc agcaggtcga gggcggccca ggattcggtc agcgtgcgcc
      601 cgagcgcgag cggcggattg tcgaagccgt cggcatcctt gccgagcagc agcacctcgg
      661 gctcgccggt cgccggattc tcggcctcgc gcagcacgcc gagcgcgcgc gcgagcagca
      721 cgtagggcag gccctcgcgg cggatgtcct cggggctcgc cacgtacagc ggcggcagct
      781 gcgcgccgtc gccctcggtg tgcagttcca gccgcgcgcg cgccggatcg ccgctgtggt
      841 tcaggcgccg ctcgtagcgc tcgcgcagct gcgcgaccgg cttcaggtag cgcagcggga
      901 acaggttgac gatgctgacc agcacgatct cgttgtcgcc gccatcggcg tcgatgacct
      961 cgacatcgcc gctgcgcgcg ccgcggaagt cggatttcag cgccgcgacg aaatcggcgt
     1021 gcgccggcgc cttcggcacg atgacggtca gcttggcgag cgcgctcggc gtgccggccg
     1081 ggatgccggg cgcggagcgg tgcatctcca gcggctcgaa ggccgtgaac gcgccggcct
     1141 gacgcacgag ttcgccgacc caggcgcggc gcgcggccgc atcgtcggca aagcgctcgg
     1201 cgaggcgctc gatgatcgac acgccgagca cgcgctcggc cggcgtgcgg atcaggctgg
     1261 cgtgcgcgag gcgcgcggcc tcctcgccgg tcttctccag cgtgtcgagc agcgtcgcga
     1321 gcggcatgcg gctgttgaag tcggcaaagc gcagcgtctc gccgaaacgc ccgaacagcg
     1381 ccgcgcgcgc gccggccgcc tgaccgcgct gcacctcggc atccaggccg aggcggtgcg
     1441 cgacctcgcg cacgcgcgcc ggatcgtagt agcggatcag gtgccggcgc aggtcggagc
     1501 cggtggcggt cgcgccgtcg gcgaggcgct gttcgaggcg ctggccgaag cgctccagcg
     1561 ccgtgcgcag gctgccggcg gcgcgctcga tcgcggtctt gagttccgcc agcgccgcga
     1621 tcagctccgg cagcaactgc tgctgcgcac gcagcgccac gccgcgcgtg cggttgacgt
     1681 acagctcctg caggtacacg gcgtgcgcgt cgagcaggct cgccggcgtg ccggtcagct
     1741 tcgcgaacac gcccatgccg gcccagtcgc gggcgttctg gttgagccgg gtctgcgcct
     1801 cgtcctcgcc ggcgcgcgcc ctggcgatgc gctcgccgag ctgcgcgtgg cgctcgtcga
     1861 gcagctcgat gacggcggcg acgaggcgcg tgcaggcatc gaggccgcgt gcgccgatgc
     1921 gccagtcgtt ggcgagttcg cgctcgatgc ggtttgcgat ctcgcgggcg atgtccttgc
     1981 gcgccttgag cttggtctcg tagaagccgg tgacgccgag gtcacggaag tcctcgtccc
     2041 agcgcttctg gcagtacttc gccagctcgt cgagccagat cgtgcgatcg agctcacgca
     2101 ccagcgactt gaagtgcggg atcgccgtct cccactccag cgtcagcgtc tgccatttcg
     2161 gcgcctgccc gtcgccggcg aggatcggca cgttcatcag caggtgatcg tcatcgagcg
     2221 cccagcgctg ctgcacgtcc ttctggcgca cgaaggcgcc ggcatcgaag ggccgcggct
     2281 cctcgacgta accgagcgcc tcctgccagt gctcgtgcgc gagcgcgagc agcgcctgac
     2341 gcgcgaagcc gtagccgagg aactcggcga tctcctcctc ggggatcgcg acgcgcttga
     2401 tgccgaaggt caggaagcgc cgggcgcgct cgccgcggcg gctcgccggt gcggtttccg
     2461 gcgagccgtc gccgttctcg gcgttctcca tgcgcgcgag cgtcggccag ccgccgtggc
     2521 gggcgcggat gatcttctgg tacaggaact cggcgacgat gcccggcagc tcctgctgca
     2581 cgtcgacggt gacgccgtgc tcgttctcgt tgccgaacac gtagcagccg ttgaacggat
     2641 cgacgagcgc gagccgcccc ttgcgcccga gcacgtcgtg cggctgccag gccccggccg
     2701 acagcgcgtt cagctccagc agcgccgcgt agccgttggc gtggtagttg ccggtatccc
     2761 agttcggcgg cgggtaggga tcgggcagca gcgcgtagac cagcacgcgg tagcgctgcg
     2821 catccgggaa cagttcgcgc agctgcacga gcgcatcgat caggctgccg ctgccggtgc
     2881 cgccggcgag cccgcacagc acgtggaagg tcgtctcgct gctgccgccg gcctgcacgc
     2941 ggcgcacctg cgtctgcagc tgttcgcggt atttgtcggc cttgcacgcg aacaggaagc
     3001 gcccgagccg gcgcttctgg ccgccgagcg tgacgccgac gatgctgttg aggatgtcgc
     3061 gccactgctc gcgcgcgccg atccacggct tgatgccggg gtagctgtcg aggtcatcga
     3121 gccgggccgc caggttggcg tcggtgatca gcagctggct cgccgccggc agctgcaccg
     3181 agacgccgag cgtcttccac gaggcatcgt ccggcgccat caactcgtcg ctggaatcga
     3241 cgtacagcca gccgagcggc acgccttcgg gctcttcgct gcggaactcc tggaagatca
     3301 gcttgcgcag cgcgcggatg accttgccgc ccgtgccccc gagcccgacg atcacatgat
     3361 tcatgcctgc ctcccggccg ccgctgtcgg gcgaaccatg cccggcgggg cgggacaggg
     3421 caagcgcggc ggatgcgcga cgccggcgcg gcgccgggcg cgcgggctac agccgtgcgg
     3481 cgccgaaggt atcgcaggcg tcgaggcgcc ccgaatccag cccggcccgg aaccagcgca
     3541 cgcgctgctg cgagctgccg tgcgtgaagc tgtcgggcac gaccgtgccg cgggcctgac
     3601 gctgcaggcg gtcatcgccg atcgcggcag cggcggtcag ggcttcctcg acatcgccgg
     3661 gttcgaggcg atgcttcgtc tgctcggcgt gccgcgccca gacgccggcc agacagtcgg
     3721 cctgcagttc gagccgcacc gacagccggt tgccgtcggc ctcgctcatg cgctcgcgcg
     3781 ccgcgtgcac gcgcccggag atgccgagca ggttctggat gtggtggccg atctcgtgcg
     3841 cgacgacgta agcctgcgcg aaatcgccgg gcgcgcggaa gcgctggcgc aggtcgtcat
     3901 agaaggacag gtcgatgtag accttgcgat ccggcggaca gtagaacggc cccatcgccg
     3961 cctggccgat gccgcaggcg gtctgcgtgg caccggagaa cagcacgagc ttcggctcga
     4021 cgtaggcctg cccggtctgc gccggcagct gcgcgttcca gacgctctcg gtgtcggcga
     4081 gcacacggcc gacgaagcgc gcctcgtcgt cgtgcgcgcg cgtctgcgcc ggccccggcg
     4141 gatgctgcac cggcgccggc gaaccgccgc cgaggatcac cgacgggtcg accccgaagt
     4201 acagcgccac cagcaccagc acgacggtgc cgatgccgcc gccgatcctg agcccgccgc
     4261 cgccgaagcc gccgcccccg ccgaaggatt caccgcggcg gtcctcgatg ttgccgctct
     4321 cgcggtcgtc gtcgccgtaa cgcatgacgt tcgcctcccc ccggatgtcc ggtgtcgtga
     4381 tgccggatcg gagccgccgc caaccgttct ggttcagtgg cggcatcggc cgggcatcga
     4441 tccggaaaag catcaggccg ccgaccctcg cggatgtcgg cggcctgatg gagcggcggg
     4501 g
//
LOCUS       DAOQIE010000306         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11425_length_4465_cov_7.668254, whole genome shotgun sequence.
ACCESSION   DAOQIE010000306 DAOQIE010000000
VERSION     DAOQIE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11425_length_4465_cov_7.668254"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..968
                     /gene="metF"
                     /locus_tag="PK144_15510"
     CDS             93..968
                     /gene="metF"
                     /locus_tag="PK144_15510"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNF67184.1"
                     /translation="MTTDLPQHSASPSASVSVSCEFFPPQTDEGLQKLLAAREALSAL
                     APEYFSVTYGAGGTTRERTFEAVHRIAATGAEAAPHLTCVGSTRAGIAGILDDYRAHG
                     IRRLVALRGDIPSGMREIGEFRYASELVAFVREHAGRHFRIEVAAYPEYHPQAASPER
                     DLDAFVHKVAAGADAAITQYFYNADAYFRFVDTVRARGVTIPVVPGIMPITNYAQLAR
                     FSDACGAEIPRWIRRKLEAFGEDRDSIRAFGLDVVAAMCERLLAQGAPGLHFYTMNLA
                     GPTLAIAERLGLSAR"
     gene            1029..1667
                     /locus_tag="PK144_15515"
     CDS             1029..1667
                     /locus_tag="PK144_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67185.1"
                     /translation="MSVRRRLSLLLPVALALLAGCATTPPPFDPGAVAGRATAWPDPL
                     AACANFSSRPEYAPLRAHLALDNLAGQTPAMRADRAKPTRSERALIRRWAGERQVCLT
                     ESVQAQRPQSSLTMIAIDEEMLASTQALDAELAAGRLSYGAYAERRQHIADHYNDDWQ
                     RAEAAWVGELNAARARRMQQRAVWGAGDPFYEDRFGGPMPGVSIGIGVGRGL"
     gene            1682..3085
                     /locus_tag="PK144_15520"
     CDS             1682..3085
                     /locus_tag="PK144_15520"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019590209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNF67186.1"
                     /translation="MRNADLVARDLAVVWHPCTQMKDHESVPLLPIVRGRGVWLEDAD
                     GRRYLDAISSWWTNLFGHAHPAINAALKDQLDALEHVLLAGCTHAPVIELSERLVALA
                     PQGLTRAFYCDNGSSAIEAALKMSFHYWLNVSGRPDKRRFVTLANSYHGETLGALAVG
                     DVALFKQTYAPLIVEALTVPSPDAYLAGPGETALDCARRAAAALDATLAAHAGEIAAL
                     IVEPLVQCAGGMRMHDPEYLRLARAACDRHGVHLIADEIAVGFGRTGTLFACEQAGIT
                     PDFLCLSKGLTGGYLPMSVVLTGDDIYRAFYDDYGKLTAFLHSHSYTGNPLAARAALA
                     TLDLFAASDVLGRNRELAAHMARLAEPFRTHPHIGDVRQRGMILAFEMVADPATRTPY
                     PWQQRRGLDVYRHALDRGALLRPLGNVVYFMPPYVITEAELDRLAEIAWECIELVTLD
                     PLPFGDIEPDGRSGWLA"
     gene            complement(3912..>4465)
                     /locus_tag="PK144_15525"
     CDS             complement(3912..>4465)
                     /locus_tag="PK144_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67187.1"
                     /translation="PRAAAVLAWSMEHVRNRPEKESTMPDYNRISAELIDQDIADLRA
                     LVTAARSKLPFLIGLSTQERKELAKLGEKTVGFDEKIQAYMLTNPEFQPGFVDPAEVR
                     KDRMLRTRFMSFFPDFMTLAEQMEDTMTILGNEILMADLAYYQSVREAARRGVPGARQ
                     IYDDLRTRFPGGGARAAAPEKTA"
BASE COUNT          628 a         1636 c         1518 g          683 t
ORIGIN      
        1 ccgcctccat accgcctggc agcaggcgac ggcgccgcat ccgagctgac gcgcccgcct
       61 cctgctgccg cctgccgaag cccgaaccgc cgatgaccac cgacctgccg cagcactccg
      121 cctcgccgtc cgcatccgta tccgtttcct gcgagttctt cccgccccag accgacgagg
      181 gcctgcagaa gctgctcgcc gcgcgcgagg cgctgtcggc gctcgcgccc gagtacttct
      241 cggtgactta cggcgccggc ggcacgacgc gcgagcgcac cttcgaggcc gtgcaccgca
      301 tcgccgcgac cggcgccgag gccgcgccgc acctgacctg cgtcggctcg acgcgcgccg
      361 gcatcgccgg aatcctcgac gactaccgcg cccacggcat ccgccggctg gtggcgctgc
      421 gcggcgacat tccctccggc atgcgcgaga tcggtgaatt ccgctacgcc agcgaactgg
      481 tcgccttcgt gcgcgagcat gccggccggc acttccgcat cgaggtcgcc gcctacccgg
      541 aataccaccc gcaggccgcg agccccgagc gcgatctcga cgccttcgtg cacaaggtcg
      601 ccgccggcgc cgatgccgcg atcacgcagt acttctacaa cgccgatgcc tacttccgct
      661 tcgtcgacac cgtgcgtgcg cgcggcgtca cgatcccggt cgtgcccggc atcatgccga
      721 tcaccaacta cgcgcagctc gcgcgcttct ccgatgcctg cggcgccgag atcccgcgct
      781 ggatccggcg caagctcgaa gccttcggcg aggaccgcga ctcgatccgc gccttcggcc
      841 tcgacgtggt cgccgcgatg tgcgagcgcc tgctggcgca gggcgcgccg ggcctgcact
      901 tctacacgat gaacctcgcc ggcccgacgc tggcgatcgc cgaacgcctc ggcctgtccg
      961 cacgctgagc cgcggcgcga tactggcctt ccggtcaccg ggccggtcac catgagtgag
     1021 tcttcgccat gtccgtgcgc cgccgtctgt cgctgctgtt gcccgtagct ctcgccttgc
     1081 ttgccggctg cgcgaccacg ccgccgccgt tcgatccggg cgcggtcgcc gggcgggcga
     1141 cggcctggcc cgatccgctc gccgcctgcg cgaacttctc gtcccgcccc gaatacgcgc
     1201 cgctgcgcgc gcatctggcg ctcgacaacc tcgccggcca gacgccggcg atgcgcgccg
     1261 atcgtgcgaa acccacccgc agcgagcgcg cgctgatccg gcgctgggcc ggcgagcggc
     1321 aggtctgcct gaccgagagc gtgcaggcgc agcggccgca gtcctcgctg acgatgatcg
     1381 cgatcgacga ggagatgctc gcctcgacgc aggcgctcga tgccgaactc gccgccgggc
     1441 gcctgagcta cggcgcctat gccgagcgcc gccagcacat cgccgatcac tacaacgacg
     1501 actggcagcg cgccgaggcc gcctgggtcg gcgagctgaa cgccgcccgc gcccggcgca
     1561 tgcagcagcg tgcggtctgg ggcgccggcg atcccttcta cgaggaccgc ttcggcggcc
     1621 cgatgccggg cgtgagcatc ggcatcggcg tcggacgcgg tctgtgagct gcaggtccgc
     1681 catgcgcaac gccgatctcg tcgcccgcga ccttgccgtc gtctggcatc cgtgcacgca
     1741 gatgaaggat cacgagtcgg tgccgctgct gccgatcgtg cgcgggcgcg gcgtctggct
     1801 cgaagacgcc gatggccggc gctatctcga tgcgatcagc tcgtggtgga cgaatctctt
     1861 cggccacgcg cacccggcga tcaacgccgc gctgaaggat cagctcgatg cgctcgaaca
     1921 cgtgctgctc gccggctgca cgcatgcacc ggtcatcgag ctgtccgagc ggctcgtcgc
     1981 gctggcgccg cagggcctga cgcgcgcgtt ctactgcgac aacggctcca gcgcgatcga
     2041 ggccgcgctg aagatgagct tccactactg gctgaacgtc agcggccgcc cggacaagcg
     2101 ccgcttcgtg acgctcgcca acagctacca cggcgagacg ctcggtgcgc tcgcggtcgg
     2161 cgacgtggcg ctgttcaagc agacctacgc gccgctgatc gtcgaggcgc tgaccgtgcc
     2221 ctcgcccgat gcgtatctcg ccggccccgg cgagaccgcc ctcgactgcg cccgccgcgc
     2281 cgccgccgcg ctcgacgcca cgctcgccgc gcacgccggc gagatcgccg cgctgatcgt
     2341 cgagccgctg gtgcagtgcg ccggcggcat gcgcatgcac gacccggagt acctgcgcct
     2401 cgcgcgcgcc gcctgcgacc gtcacggcgt gcacctgatc gccgacgaga tcgcggtcgg
     2461 cttcgggcgc accggcacgc tgttcgcgtg cgagcaggcc ggcatcacgc cggatttcct
     2521 gtgcctgtcg aagggcctga ccggcggcta cctgccgatg tcggtggtgc tgaccggcga
     2581 cgacatctac cgcgccttct acgacgacta cggcaagctg accgccttcc tgcactcgca
     2641 cagctacacc ggcaatccgc tggcggcgcg cgcggcgctg gcaacgctcg atctgttcgc
     2701 cgcgagcgac gtgctcggcc gcaaccgcga actggccgcg cacatggcaa ggcttgccga
     2761 gccgttccgc acgcatccgc acatcggcga cgtgcgccag cgcggcatga tcctcgcgtt
     2821 cgagatggtc gccgacccgg ccacgcgcac gccgtatccg tggcagcagc gccgcggcct
     2881 cgatgtctac cggcatgcgc tcgatcgcgg cgcgctgctg cgcccgctcg gcaacgtcgt
     2941 ctacttcatg ccgccgtacg tgatcaccga agccgaactc gaccggctcg ccgagatcgc
     3001 gtgggagtgc atcgaactgg tgacgctcga tccgctgccc ttcggcgaca tcgaacccga
     3061 cgggcgcagc ggctggctgg cgtgacggcg acgctggcgg atggcgtcga gaacgccggc
     3121 gcgggatcgc acggcgcctg aacgccactt ccgggatgcc ggcttgcccg cctcaagccc
     3181 gcccacgcac cgcgaccagt gccgtaagcc actgactttt ctctgcgaat gctgtcgcgc
     3241 aggccccgcc cggcccgcgt gcggcggcac gattcgccgt gcgcaagcgg gcagggacac
     3301 gcccctgcgc ctgagcgtcg ctgtcgccgc cctctcccga agtcccgact tcaccccgcg
     3361 ctggggcgcc gtcgccccag aatgagtcga cgccgatagt tgaagtatcg gcgtcgatgc
     3421 cccggcatcg gcgccggtat gtggaacacc ggcgccggtg gttcagatat cggcttcgat
     3481 acctgaagtg tcggcgccgg tacttcagca gtcccccgcc ctgctcaggt gtcggcgtcg
     3541 atacctgaac aaccggcgcc gcggtaaaaa agggcggcgg cgctgcaaaa aggggcggcg
     3601 caggggtgcg gacccctgac cgggatgtgc agaccttcgt tgccgaggtg ccggacctcg
     3661 acggcggggt ttccgcaagc cggcgcggga ctggcggcac cggcgccggc cgtcgggatc
     3721 gcggcggcgg tgatgccggc atcgatgccg gcagttcgga taccggcgcc gatacgacgg
     3781 gtatcggtgc cgggattcgc agcagcggcg ccgggatcga ggggctcgac cgcaatggat
     3841 cggctggcgc gtccgatcgc tgcaagggcg gcggcgggtg ttgcggaccc gccgccgggg
     3901 tggcgagggg atcaggcggt cttctcgggg gccgcggccc gggcgccgcc gccggggaag
     3961 cgggtgcgca ggtcgtcgta aatctgccgg gctcccggca cgccgcggcg ggcggcctcg
     4021 cgcacgctct ggtaataggc gagatcggcc atcaggattt cattgccgag gatggtcatc
     4081 gtgtcttcca tctgctcggc gagcgtcatg aaatccggga agaagctcat gaagcgcgtg
     4141 cgcagcatgc ggtccttgcg gacttccgcg gggtcgacga aacccggctg gaattccggg
     4201 ttggtgagca tgtaagcctg aatcttctcg tcgaagccaa cggtcttctc accgagtttc
     4261 gcgagttcct tgcgctcctg cgtactcagg ccgatcaaaa acggcaactt gctgcgggct
     4321 gcggtcacga gtgcgcgcag gtcggcgatg tcctggtcga tcagttcggc ggaaatgcgg
     4381 ttgtagtcgg gcatggtgga ttccttttca ggacggttgc ggacgtgttc catactccat
     4441 gcaaggacgg ccgcagcgcg gggtg
//
LOCUS       DAOQIE010000307         4460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11453_length_4460_cov_8.195006, whole genome shotgun sequence.
ACCESSION   DAOQIE010000307 DAOQIE010000000
VERSION     DAOQIE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11453_length_4460_cov_8.195006"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..548)
                     /gene="sodC"
                     /locus_tag="PK144_15530"
     CDS             complement(9..548)
                     /gene="sodC"
                     /locus_tag="PK144_15530"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HNF67188.1"
                     /translation="MNKRLSLTLAAALTAAAGSAALAADSITISMDQLAADGGMTPAG
                     IVVATPAADGKGVVFKPELKGLPPGEHGFHVHEKPDCGPAEKDGKKVPGGNAGGHYDP
                     DKTGRHAGPTGDGHLGDLPALVVAADGTATAPVTAPRLKMSDLSGRSLMIHAGHDNYA
                     DQPQPLGGGGARMLCGVIR"
     gene            763..1419
                     /locus_tag="PK144_15535"
     CDS             763..1419
                     /locus_tag="PK144_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HNF67189.1"
                     /translation="MDQNEKSLIDDLYRRLQLAEQKTGPRDAQAEAVIREHFSRQPGV
                     AYYMTQAMLVQEHALKSLNERVQQLEQELASRPAAGGGSFLSGLFGGGASSAPAGRAG
                     IPPYQPQPGAFQQAPRGGFLSGALQTAAGVAGGVLLGHALMSMFSDVFGDSGAEAVAA
                     AVPEAAENFDLASAAEDIPSSGGFLGDAGSGFGGFGDSTASADNDFGGFGGDDDDQFV
                     "
     gene            1831..3600
                     /locus_tag="PK144_15540"
     CDS             1831..3600
                     /locus_tag="PK144_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNF67190.1"
                     /translation="MDPSLNPQSVSIVLCGSGGAGVMTAGQMLLDAAAAAGLYGVMSR
                     ALGPQIRGGEAAALLRLANHPVEAPDDAFDVLLAVDWQNIERFAAELPLRADSLVIGD
                     PAAGELPAAILRAHPRRAEVALQALADETGSRVNMVALGLLAELIGLPAEAVYATLAA
                     ALADKGEAVVESGIRAALAGAQVVAALPAVPRLPAVAAAANRPVRWSLSGNEASGLGA
                     LRGGVRFVAAYPITPATEILEWLAPRLPPLGGCLVQAEDELASVNMILGGAFGGVPSL
                     TATSGPGLALMTESLGLGVASETPVVVCNVMRGGPSTGIPTKSEQSDLDIALHGLHGD
                     APHLVTAATGIADCAFTTQWTVQLAEALQVPAIVLSDQAMGQSRAVVDALPDPGFRAV
                     RLTAPAGVEGYQRYAVTESGVSPMAVPGTPGGSFVADGLEHAPRGTPSTQAAHHREQL
                     DKRHRKLEVHDYGEHWALLEGARDAETAVICFGSLTAAAREAVDRLNAAGTPVRLISL
                     RLLAPALPLAFAAALRGVRHALVVEQNHSRQFLGHLRSHYPLPVHTHGYHRPGPVAIR
                     PGEIVAAVRSLLAPVEAPVTVED"
     gene            3606..4460
                     /locus_tag="PK144_15545"
     CDS             3606..4460
                     /locus_tag="PK144_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNF67191.1"
                     /translation="MNDCKLIPAPAPLTAKDYRSDLKPVWCAGCGDYAVIAAITRALA
                     ELQLPREDVAFVSGIGCSSRIPAYSAVYGFHGVHGRALPLATGLKVARPDLTVLVAGG
                     DGDGFSIGGNHFLHACRRNADVTYIVMDNSVYGMTKGQASPTTAADWTGSVLTPDGPG
                     VPAFRPLEIALAAGASFIARAFSGDPTTLAAQIVAGIRHEGFSLIHVLCPCVTYRPEQ
                     QKEFKARVHAEWQPTDDRREAFGRLLDDDGYATGILYRNPRPAYRAPQVAATTLDAIA
                     AGFEVAAA"
BASE COUNT          586 a         1709 c         1516 g          649 t
ORIGIN      
        1 cggcgccgtc agcggatcac gccgcagagc atgcgggcgc cgccgccgcc gagcggctgc
       61 ggctggtcgg cgtagttgtc atgaccggcg tggatcatca gcgagcggcc ggacaggtcc
      121 gacatcttca gccgcggtgc cgtcaccggc gcggtcgccg tgccgtcggc ggcgacgacc
      181 agcgccggca ggtcgccgag gtggccgtcg ccggtcgggc cggcatgccg gccggtcttg
      241 tccggatcgt aatgcccgcc ggcattgccg cccggtacct tcttgccgtc cttctcggcc
      301 gggccgcagt cgggcttctc atggacgtgg aaaccgtgct cgccgggcgg caggcccttc
      361 agttcgggct tgaacaccac gcccttgccg tcggcggccg gcgtggcgac gacgatgccg
      421 gccggcgtca tgccgccgtc ggcagcgagc tggtccatcg aaatcgtgat cgagtcggcc
      481 gcgagtgcgg cactgccggc cgcagcggtc agggcggcgg ccagcgtcag actcagtcgc
      541 ttgttcatgg aaactcctca ttgcttgtgg tcatggtcag ccgggggcag gccggcttag
      601 ggacagctta ggcgcccgtg cggttcgctc ctgccagcga acacggtgcc cggcgagcgg
      661 tctgaagcgc caccccgtac cggaccgccg agccgggcga gctttacagg taagaaatca
      721 cctgatagaa aattgcctcg gcacctgcac cggagtccaa ccatggacca gaacgaaaaa
      781 tccctgatcg atgacctgta ccgccgcctg caactggccg aacagaagac cggcccgcgc
      841 gatgcccagg ccgaagccgt gatccgtgag cacttcagcc gccagccggg cgtcgcgtac
      901 tacatgacgc aggcgatgct ggtgcaggag cacgcgctga agagcctcaa cgagcgcgtg
      961 cagcagctcg aacaggaact cgcctcgcgc ccggccgccg gcggcggcag cttcctgtcc
     1021 ggcctgttcg gcgggggcgc gagcagcgcg ccggccggtc gtgccggcat tccgccctac
     1081 cagccgcagc cgggcgcctt ccagcaggcg ccgcgcggcg gcttcctgtc cggcgcgctg
     1141 cagaccgcgg ccggcgtcgc cggcggcgtg ctgctcggcc acgcgctgat gagcatgttc
     1201 tccgatgtat tcggtgacag cggcgccgaa gcggtcgccg cggccgtgcc ggaagcggcc
     1261 gagaacttcg atctggcctc ggcggccgag gacatcccgt cgagcggcgg cttcctcggc
     1321 gacgccggca gcggcttcgg cggcttcggc gattccaccg cctcggccga caacgatttc
     1381 ggcggtttcg gcggcgacga cgacgaccag ttcgtctgag cgtcccgcca cccggcggcg
     1441 ccctccaccg cggcgccgcc tgctttttca tccggcttca tccgtttcat ccgcttgtct
     1501 tgccccggcc gccgccgttg ccgtccaccc tcccgcccgg cagcgacggc ggccggttct
     1561 ttccggctgc acccccctag tccgcccctt gaccacccct tgcaccgcca cagccccccg
     1621 gactggtgca tcgcgcaccg caatggtgcg cacaacttct tgatatggat cagcattttg
     1681 tcggatgcgt ccgctcgcac gggcagaagt tgtcgcagcg caatatttcg ccgccccgcc
     1741 ggtgctgaaa tgccccgcag cggccgaccc gccagcgggt tggcctttca tttcagggct
     1801 ttgccttagc tgcgagaggt cgcgagactc atggacccat cactgaatcc gcaatcggtt
     1861 tccattgtgc tgtgcggctc cggcggcgcc ggcgtcatga ccgccgggca gatgctgctc
     1921 gacgcggccg ccgccgccgg cctgtacggc gtgatgtcgc gggccctcgg cccgcagatc
     1981 cgcggcggtg aggccgccgc gctgctgcgg ctggcgaatc atccggtcga ggcgcccgac
     2041 gatgccttcg acgtgctgct cgccgtcgac tggcagaaca tcgagcgctt cgccgccgag
     2101 ctgccgctgc gtgccgacag tctggtcatc ggcgatccgg ccgccggcga gctgccggcg
     2161 gcgatcctgc gcgcgcatcc gcgccgcgcc gaagtcgcgc tgcaggcgct ggcggacgag
     2221 accggcagcc gcgtcaacat ggtcgcgctc ggcctgctcg ccgaactcat cggcctgccg
     2281 gccgaggccg tgtacgcgac gctcgccgcg gcgctcgccg acaagggcga ggccgtggtc
     2341 gagtccggca tccgcgccgc gctcgccggg gcgcaggtgg tcgcggcgct gccggccgtg
     2401 ccgcggctgc cggccgtcgc cgccgccgcg aaccggccgg tgcgctggtc gctgagcggc
     2461 aacgaggcct cggggctcgg ggcgctgcgc ggcggcgtgc gcttcgtcgc cgcgtatccg
     2521 atcacgccgg cgaccgagat cctcgaatgg ctggccccgc gcctgccgcc gctcggcggc
     2581 tgcctggtgc aggccgagga cgaactggcc tcggtcaaca tgatcctcgg cggcgccttc
     2641 ggcggcgtgc cgtcgctgac cgcaacctcc gggccgggcc tggcgctgat gaccgaatcg
     2701 ctcggcctcg gcgtggcctc cgaaaccccg gtcgtggtct gcaacgtcat gcgcggcggc
     2761 ccgtcgaccg gcatcccgac caagtccgag cagtccgacc tcgacatcgc gctgcacggc
     2821 ctgcacggcg atgcaccgca cctcgtcacc gccgccaccg gcatcgccga ctgcgcgttc
     2881 acgacgcagt ggaccgtgca gctcgccgag gcgctgcagg tgccggcgat cgtgctgagc
     2941 gatcaggcca tgggccagtc gcgcgccgtc gtcgatgcgc tgcccgatcc gggtttccgc
     3001 gccgtgcgcc tgaccgcgcc ggccggcgtc gagggctacc agcgctacgc cgtcaccgaa
     3061 tccggcgtct cgccgatggc cgtgcccggc acgccgggtg gcagcttcgt cgccgacggc
     3121 ctcgaacacg cgccgcgcgg cacaccgtcg acgcaggccg cgcaccaccg cgaacagctc
     3181 gacaagcgcc accgcaagct cgaagtccac gactacggcg agcactgggc gctgctcgaa
     3241 ggcgcacgcg acgccgagac cgccgtgatc tgcttcggct cgctgaccgc cgcggcgcgc
     3301 gaggcggtcg accggctcaa cgccgccggc acgccggtgc ggctgatctc gctgcgcctg
     3361 ctcgccccgg cgctgccgct cgccttcgcc gcggcgctgc gcggcgtgcg ccatgcgctc
     3421 gtcgtcgaac agaaccacag ccggcagttc ctcggccatc tgcgcagcca ctacccgctg
     3481 ccggtgcaca cgcacggcta ccaccggccc ggcccggtcg cgatccgccc cggcgagatc
     3541 gtcgccgccg tccgctccct gctcgcgccg gtcgaggcgc ccgtgaccgt cgaggactga
     3601 ccgccatgaa cgactgcaag ctcatccccg cgccagcccc gctgactgcc aaggactacc
     3661 gctccgacct caagccggtc tggtgcgccg gctgcggcga ctacgccgtc atcgccgcga
     3721 tcacgcgcgc gctcgccgaa ctgcagctgc cgcgcgagga cgtggccttc gtctccggca
     3781 tcggctgctc gtcgcgcatc ccggcgtact cggccgtcta cggcttccac ggcgtgcacg
     3841 gccgggcgct gccgctcgcc accggcctca aggtcgcgcg gcccgatctg accgtgctgg
     3901 tcgccggcgg cgacggcgac ggcttctcga tcggcggcaa tcacttcctg cacgcctgcc
     3961 ggcgcaacgc cgatgtgacc tacatcgtca tggacaacag cgtctacggc atgaccaagg
     4021 gccaggcctc gccgaccacg gccgccgact ggaccggcag cgtgctgacg cccgacggcc
     4081 ccggcgtgcc ggcgttccgg ccgctggaga tcgcactcgc cgccggcgcg agcttcatcg
     4141 cccgcgcctt ctccggcgat ccgaccacgc tggccgcgca gatcgtcgcc ggcatccgcc
     4201 acgaaggctt ctcgctgatc cacgtgctct gcccgtgcgt gacctaccgg cccgagcagc
     4261 agaaggagtt caaggcgcgc gtacacgccg aatggcagcc gaccgacgac cgccgcgaag
     4321 ccttcggccg cctgctcgat gacgacggct acgcgaccgg catcctgtac cgcaacccgc
     4381 gcccggccta ccgcgcgccg caggtggcag cgacgacgct ggacgcgatc gccgccggct
     4441 tcgaggtggc ggcggcctga
//
LOCUS       DAOQIE010000308         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11458_length_4459_cov_7.013397, whole genome shotgun sequence.
ACCESSION   DAOQIE010000308 DAOQIE010000000
VERSION     DAOQIE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_4459_cov_7.013397"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="PK144_15550"
     CDS             <1..1230
                     /locus_tag="PK144_15550"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNF67192.1"
                     /translation="AILRRWFDLIVTHADDLARMITAECGKPLAEAMGEVGYGASFVE
                     WFAEEAKRAYGETIPGVVGDKRLITIKQPIGVCAAITPWNFPLAMITRKVAPAIAAGC
                     TVVVKPAEQTPLTALALAKLAHLAELPPGVFNVVTGDPVAIGSVMTASPAVRHLSFTG
                     STEVGRLLMAQSAPTIKKLALELGGNAPFIVFDDADVDAAVEGALIAKYRNTGQTCVC
                     ANRFLVQSGVYEAFAQKLAARVAMLQVGPGSEPDVVQGPLIDTAGLEKVEAHLADATA
                     KGARILTGGRRHARGGTFFEPTVLAGVTTAMRVAREETFGPVAPLFRFDTEAQAVEMA
                     NATEFGLAAYFYSRDIGRCFRVAEALEYGMVGVNTGLISNEVAPFGGVKQSGIGREGS
                     KYGLEEYLEIKYLCVAV"
     gene            1238..1906
                     /locus_tag="PK144_15555"
     CDS             1238..1906
                     /locus_tag="PK144_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67193.1"
                     /translation="MKFIGLALLLLVLFIGYRLWQRAHRRDRAAFIDAFPYRKTLDRR
                     LAGKRPELTPEQREQVFAGLREYFHLSNDAGRNMVAMPSQVVDDAWHEFILFTRQYRR
                     FCDRAFGRFLHHTPAEAMASPTVAQDGIKRAWRLACAREGINPKTPSRLPLLFAIDGV
                     LGIANGFRYQLDCMATGKAATGTTGGDFCASHIGCSSGCSGGSGSSDGDGGSGCGGGC
                     GGGD"
     gene            complement(1934..2818)
                     /locus_tag="PK144_15560"
     CDS             complement(1934..2818)
                     /locus_tag="PK144_15560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNF67194.1"
                     /translation="MSVESTELEVNSLTPANTYSRRTFIVTSLGAGFALGVQPVMAQT
                     SISTPADGLVAGEIKVAAQGGEMVAYRAAPAGAISPPVILVVQEIFGVHEYIRDICRR
                     LAKLGYLAIAPELFARQGDPRQYSAIPDLMSNIVSKVPDAQVMADLDACVAWAKANGG
                     DTSRLGITGFCWGGRITWLYSAHNPQVKAGVAWYGRLASPVTENNPKHPVDLAGQLHA
                     PVLGLYGGADQGIPLDTVEKMRAGLSAGGKAACASKIHVYEGAPHAFHADYRPSYRKA
                     EAEDGWQRMLAWFKRNGI"
     gene            2902..3333
                     /locus_tag="PK144_15565"
     CDS             2902..3333
                     /locus_tag="PK144_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNF67195.1"
                     /translation="MNKIGGYNIEDLQPGMSASVSKTVTEADIIMFAGVSTDVNPAHL
                     DEEYAKGTQFGGRIAHGMLSASFISAVLANKLPGAGTIYLGQTLKFKAPVRIGDTVTA
                     TVTVREVVVDKRRCILDAVCTVNGKTVIEGESTMYCTSEKA"
     gene            3339..3923
                     /locus_tag="PK144_15570"
     CDS             3339..3923
                     /locus_tag="PK144_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67196.1"
                     /translation="MRLQRALLTSFISLLLASGASAQLMDENLLQGMPAGYKIGFQER
                     QGPIIITEMVPESESAEEWTEMVTSQLFIGLKSVSPETFRAESRKKWLEACKDGNFAE
                     IASGEENGYPMALWMLSCPHSKAPGRPEITWFKAIRGQDAFYVVQKSFRFEPSNEQVQ
                     QSMRYLRQISVCDTRIPQRACPVIKPPEAEGARN"
     gene            complement(3950..>4459)
                     /locus_tag="PK144_15575"
     CDS             complement(3950..>4459)
                     /locus_tag="PK144_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNF67197.1"
                     /translation="FKTNLEAAQTIARQLRLRNLGGIIIIDFIDMDTAEHRDAVLAEF
                     NKALARDHTRLTVNGFTALGLVEMTRKRTRESLAHVLCEPCPTCDGRGEVKTARTVCY
                     EILRELLREARQFNAREYRVIGAPSVIDLFLDEESQGLAMLSDFIEKPVSLQAEPSYS
                     QEQYDIVLM"
BASE COUNT          756 a         1432 c         1495 g          776 t
ORIGIN      
        1 gcgatcctgc gccgctggtt cgacctgatc gtcacgcatg cggacgatct tgcccgcatg
       61 atcaccgccg agtgcggcaa gccgctggcc gaagcgatgg gtgaggtcgg ctacggtgct
      121 tccttcgtcg aatggttcgc cgaggaggcg aagcgcgcct acggcgagac gattcccggc
      181 gtggtcggcg acaagcgcct gatcacgatc aagcagccga tcggcgtctg cgccgcgatc
      241 acgccgtgga acttcccgct ggcgatgatc acgcgcaagg tggcgccagc gatcgccgcc
      301 ggctgcaccg tggtggtcaa gccggccgaa cagacgccgc tgaccgcgct cgcactggcc
      361 aagctggccc acctcgccga actgccgccg ggcgtcttca acgtcgtcac cggcgacccc
      421 gtcgcgatcg gcagcgtcat gaccgcgagc ccggcggtgc gccacctttc cttcaccggc
      481 tcgaccgagg tcggccgcct gctgatggcg cagagcgcgc cgacgatcaa gaaactggcc
      541 ctcgaactcg gcggcaacgc gcccttcatc gtcttcgacg atgccgatgt cgatgccgcg
      601 gtcgaggggg cgctcatagc caagtaccgc aacaccggcc agacctgcgt ctgcgccaac
      661 cgctttctgg tgcagtccgg cgtttatgag gcctttgcgc agaagctcgc agcgcgcgtg
      721 gcgatgctgc aggtcggccc cggcagcgag ccggacgtcg tgcagggacc gctgatcgat
      781 acggccggac tggagaaggt cgaggcccac ctcgccgatg ccaccgcgaa gggcgcgcgc
      841 atcctcacgg gtggcaggcg ccatgcgcgc ggcggcacct tcttcgaacc gaccgtgctc
      901 gccggcgtga cgactgcgat gcgcgtggcg cgcgaggaaa ccttcgggcc ggtggcgccg
      961 ctgttccgtt tcgatacgga agcgcaggcg gtcgaaatgg ccaatgccac ggaattcggc
     1021 ctcgccgcct acttctactc gcgcgatatc ggacgctgct tccgcgtggc cgaggcgctg
     1081 gagtacggca tggtgggcgt caataccggc ctcatctcga acgaggtggc gccgttcggc
     1141 ggcgtcaagc agtccggcat cgggcgcgag ggctcgaaat acgggctcga ggaatacctg
     1201 gagatcaagt acctctgtgt cgcggtctga gccggcgatg aagttcatcg gccttgccct
     1261 gcttctgctg gtgctgttca tcggctatcg gctctggcag cgtgcgcacc gaagggaccg
     1321 tgctgccttc atcgatgcct ttccttaccg gaagacgctt gaccgccgcc tcgccgggaa
     1381 gcggccggaa ctgacacccg agcaacgcga gcaggttttt gccggcctgc gcgaatactt
     1441 ccatctgtcc aacgatgccg gccgcaacat ggtggcgatg ccgtcgcagg ttgtcgatga
     1501 cgcatggcac gagttcatcc tgttcacgcg ccagtaccgg cgcttctgcg accgcgcctt
     1561 cggccgcttc ctgcaccata ccccggcgga agcgatggcc tcgccgaccg tggcacagga
     1621 cggcatcaag cgtgcctggc gactggcctg cgcacgcgag ggcatcaacc cgaagacgcc
     1681 ctcgcgactg ccgctgctct ttgcgatcga cggtgtgctg ggtatcgcca atggcttccg
     1741 ctaccagctt gactgcatgg cgacgggcaa ggctgcgaca ggtacgaccg ggggagactt
     1801 ctgcgccagc cacatcggct gcagcagcgg ctgctcgggg ggttccggca gcagcgatgg
     1861 cgatgggggc tccggctgtg ggggcggttg cggcggtggc gactgatctc gccacgccgc
     1921 gccggcgacg ggctcagatc ccgttgcgct tgaaccaggc cagcatgcgc tgccagccgt
     1981 cctcggcttc cgccttgcgg tagctcggcc ggtagtcggc atggaaggcg tgcggggctc
     2041 cctcgtagac gtggatcttc gaggcgcagg ccgccttgcc gccggcggac agcccggcgc
     2101 gcatcttctc caccgtatcg agcggaatgc cctggtcggc gccaccatag agaccgagta
     2161 ccggcgcgtg caactggccg gcgaggtcga ccgggtgctt cggattgttc tccgtgaccg
     2221 gcgaggcgag gcggccgtac caggccacac cggccttcac ctgcgggttg tgggcgctgt
     2281 acagccaggt gatgcggccg ccccagcaga aaccggtgat gccgagacgc gaggtgtcgc
     2341 cgccgttggc cttggcccag gcgacgcagg cgtcgagatc cgccatcacc tgcgcgtccg
     2401 gtaccttcga gacgatgttg ctcatcaggt ccgggatcgc cgagtactgg cgcggatcgc
     2461 cctggcgcgc gaacagttcc ggcgcaatcg ccaggtagcc gagtttcgca agccgccggc
     2521 agatgtcccg gatgtattcg tgaacgccga agatctcctg cacgacgagg atcaccggcg
     2581 ggctgatcgc gccggcgggc gcagcgcgat aggcgaccat ctcgccgccc tgcgcggcaa
     2641 ccttgatttc accggcgacg aggccgtcgg ccggggtgct gatggatgtc tgtgccatga
     2701 ccggttgcac gccaagggca aagccggcac cgagactggt aacgatgaat gtgcgacggg
     2761 agtaggtatt tgcgggggtc aggctgttga cttcgagttc ggtgctttcg acgctcatcg
     2821 attgtctcct cgcggacatt gtcatgggga atgcagcgtg tatgctaccc ctctcggaca
     2881 agaacccagg agatgagttt catgaacaag atcggcggct acaacatcga ggacctgcaa
     2941 cccggcatga gcgccagcgt gtcgaagacg gtgacggaag cggacatcat catgttcgcc
     3001 ggtgtctcga ccgacgtgaa tccggctcac ctcgacgagg aatatgccaa ggggacccag
     3061 ttcggcggcc gcatcgcgca cggcatgctc tcggcgagct tcatttctgc cgtgctcgcc
     3121 aacaagctgc cgggtgccgg cacgatctac ctcggccaga cgctcaagtt caaggcgccg
     3181 gtgcgcatcg gtgataccgt caccgcgacc gtgacggtgc gcgaggtcgt cgtcgacaag
     3241 cgccgctgca tcctcgatgc cgtctgcacg gtcaacggca agaccgtcat cgaaggtgaa
     3301 tcgacgatgt actgcacgag cgaaaaggcc tgatcgcgat gcgcctgcag cgcgccctcc
     3361 tgacctcgtt tatcagcctg ctgctggcga gtggcgcgtc ggcgcaactg atggacgaga
     3421 acctcctgca gggcatgccg gccggctaca agatcggatt ccaggaacgc caggggccga
     3481 tcatcatcac cgaaatggtt ccggaatccg aatccgccga ggaatggacc gagatggtca
     3541 ccagccagct cttcatcggc ctgaagtcgg tcagcccgga gacctttcgc gccgagtccc
     3601 gcaagaagtg gctggaagcg tgcaaggacg gcaatttcgc ggaaatcgcc agcggcgagg
     3661 agaacggtta cccgatggcc ctgtggatgc tttcgtgtcc ccattcgaag gcgccggggc
     3721 gcccggagat cacgtggttc aaggcgatcc gcggacagga tgccttctac gtcgtgcaga
     3781 aatccttccg cttcgagccg tcgaacgaac aggtgcagca gtcgatgcgc tacctgcggc
     3841 agatcagtgt ctgtgacacc cgtatcccgc agcgtgcctg cccggttatc aagccgccgg
     3901 aggcagaggg tgcccggaac tgaaaacggg gccgtggccc cgtttttcat tacatcagca
     3961 cgatgtcgta ctgttcctgc gaatagctgg gttctgcctg cagcgagacc ggcttctcga
     4021 tgaagtcgga gagcatcgcc agaccctgcg attcttcgtc gaggaagagg tcgatcacgc
     4081 tcggtgcacc gatgacgcgg tattcgcgag cgttgaactg gcgcgcctcg cgcagcaatt
     4141 cccggaggat ttcgtagcag acggtacgcg ccgtctttac ctcgccacgg ccatcgcagg
     4201 tcgggcaggg ttcgcagagc acgtgcgcca gcgattcccg ggtgcgcttg cgcgtcatct
     4261 cgacgaggcc gagcgcggtg aagccgttga ccgtcaggcg cgtgtggtcg cgcgccagcg
     4321 ccttgttgaa ctcggcgagc acggcgtcgc gatgctcggc cgtatccatg tcgatgaaat
     4381 cgatgatgat gatgccgccg aggttgcgca ggcgcagctg gcgggcgatg gtctgcgccg
     4441 cctcgaggtt ggtcttgaa
//
LOCUS       DAOQIE010000309         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11519_length_4445_cov_7.032574, whole genome shotgun sequence.
ACCESSION   DAOQIE010000309 DAOQIE010000000
VERSION     DAOQIE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11519_length_4445_cov_7.032574"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK144_15580"
     CDS             <1..611
                     /locus_tag="PK144_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67198.1"
                     /translation="ARFDFGLPPERIAGAARPAPAQLAAPRNPMLRVRGSVRDPAGRW
                     QPRQFDVRLAAGAIVPLPLYPAPSGTPASSGGRLFGSLRLPNGAAAAWARLELRVTVA
                     PGRSVSAVAQADGNGDFVLAAARVPLPPANVASFPAELRVFHRGGSWPDGLNPDTQQP
                     WTLLSATADSFAEVYAFDFPPGAALRPLGFQRQTLVIRAPQP"
     gene            656..2947
                     /locus_tag="PK144_15585"
     CDS             656..2947
                     /locus_tag="PK144_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNF67199.1"
                     /translation="MPEYLAPGVFVEETSFRHKSIEGVGTSVAALVGPTRYGPLRGAP
                     EVVTSLTEYTRIYGDGADLVFDGVGEPNYTAIAARAFFDNGGKQLFVSRVCRDVNRAD
                     AVNDGSGAVRAVAAAPGARLRFEARFPGAAGNFTLEVRWRDAGNLLRTGRIPAAAAAA
                     LAAGESALIDTTVPVAARVNGSLLADGRFPLQASGFATLENRPNDPNDAAAGSTRVWR
                     LRADARVLGADGVALSAAERTQLGDVLVAAALPDTAGYTRVSVRAPASGPLVANEPVV
                     LVLAGSDAAVNLAPVLSGQNNPSNGRRRVLRGTLNAAGTVLTLTAADNRVMAADQPVA
                     ELSLSADATLALAALATLPDTGAALFALRAFDLDVLSAPDPVAPERAPEPVFAVADLS
                     PSPQAVRHLAALLPMQPARRAERLTQPVAALYLNGEPSATQVIDSIFALADTVALLPA
                     AGALAGPRWLIHMSGGSDGPLPGAADYTGESDELTGSTGFAALEEIEDIAIVATPAAA
                     AHPASHAQVVQALWAHCRRMRYRVGIVDAEAGMSLNEVRAFAGQFSDSLLALYYPWVV
                     TADPSGVRPELTVPPGGFIAGVYAGTDVRRGVHKAPANEVLIGVTGLETDVNRFRQEL
                     LNPNGINCLRFFPGRGYRVWGARTLSDDPEWRYVNVRRYFLFLERSIEKSTQWAVFEP
                     NGEALWANVRSTVEDFLYAEWTAGHLLGASTKEAYFVRCDRSTMTQNDIDNGRLVCLI
                     GVAPLRPAEFVIFRIGQKTADAR"
     gene            2964..3422
                     /locus_tag="PK144_15590"
     CDS             2964..3422
                     /locus_tag="PK144_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNF67200.1"
                     /translation="MPVLRDTPYTAFNFLVEIEPGIGRELRAGFSDVSGLNNEITVAE
                     YRAGNSKVNHVQKRPGVYKAGDVTLKRGIIGATDLWDWIVATREGRVSEAKRTVVVSL
                     LAENRDDVVVRWKLQGAMPMKWTGPTLAGKGGGDVAIEELVLSVESVDQE"
     gene            3430..>4445
                     /locus_tag="PK144_15595"
     CDS             3430..>4445
                     /locus_tag="PK144_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67201.1"
                     /translation="MLTFADARVFLNPAPVAPRQPVLPVALVGHVPGAARPERLSRAL
                     REPWHGLGLQQREQQLDHALANGPAGDDDDALLALHHLPLALDSHAQFLDLFPDAGTA
                     ATGPAAPVGAAPWLPLAIEDFFAAGGERLWLVRVPQSEGRDGLLPAQPGDPARPDTLR
                     GLDVALNVPDIGLVALPDLERLQLPAGLDPLPDFTPAPAQARFVPVDELARADAVPTP
                     PPSPLSPPWPLAEVVRRLCARFEAWRPDVQALLTLPLAATLDGGAQPDAAALAWLAAL
                     DGEELRRAQRLQPLYPYLRGRRAPLASATGLVAGTIAASAFRDGPWRSVAGRDLGGDA
                     LPWPV"
BASE COUNT          552 a         1723 c         1574 g          596 t
ORIGIN      
        1 gcgcccgctt cgacttcggc ctgccgcccg agcgcatcgc cggtgcggcc cgcccggcgc
       61 cggcgcagct cgcggcgccg cgcaatccga tgctgcgcgt gcgcggctcg gtccgcgatc
      121 cggccggacg ctggcagccg cggcagttcg acgtgcgcct cgccgccggc gccatcgtgc
      181 cgctgccgct gtatccggcg ccctccggca cgccggcgag cagcggcggt cgcctgttcg
      241 gcagcctgcg cctgcccaat ggcgccgctg cggcctgggc gcggctggag ctgcgcgtga
      301 cggtggcgcc ggggcgcagc gtcagcgccg tcgcgcaggc cgatggcaac ggtgatttcg
      361 tgctcgccgc cgcgcgcgtg ccgctgccgc cggccaacgt cgcgagcttt ccggccgaac
      421 tgcgcgtgtt ccaccgcggc ggcagctggc cggacggcct caatcccgac acccagcagc
      481 cgtggacgct gctgtcggcc accgccgaca gcttcgccga ggtctacgcc ttcgattttc
      541 cgcccggcgc cgcgctgcgc ccgctcggct tccagcgcca gacgctggtg atccgcgcac
      601 cccagccctg accctgcatc agccgacaag accgtcgcca cgaggaggcc gcgtcatgcc
      661 cgagtatctc gccccaggcg tgttcgtcga ggagacgagc ttccggcaca agtcgatcga
      721 aggcgtcggc accagcgtcg cggcgctggt cgggccgacg cgctacgggc cgctgcgcgg
      781 tgcgcccgaa gtcgtcacca gcctgaccga gtacacgcgc atctacggcg acggcgccga
      841 tctggtgttc gacggcgtcg gcgaaccgaa ctacacggcg atcgccgcgc gggcgttctt
      901 cgacaacggc ggcaagcagc tcttcgtcag ccgcgtctgc cgcgacgtga accgcgccga
      961 cgccgtcaac gatggcagcg gtgccgtgcg cgccgtcgcc gctgcgcccg gcgccaggct
     1021 gcgcttcgag gcgcgctttc cgggcgcggc cggcaacttc acgctggagg tccgctggcg
     1081 cgatgccggc aacctgctgc gcaccggccg catcccggcc gcggcggccg cggcgctggc
     1141 ggccggcgaa tcggcgctga tcgacaccac ggtgccggtc gccgcgcgcg tgaacggctc
     1201 gctgctcgcc gatggccgtt ttccgctgca ggcgagcggc ttcgcgacgc tggagaaccg
     1261 cccgaacgat ccgaacgatg ccgcggccgg cagcacgcgc gtctggcggc tgcgggccga
     1321 tgcgcgcgtg ctcggcgccg acggcgtggc gctgagcgcc gccgaacgca cgcagctcgg
     1381 cgacgtgctg gtggcggcgg cgctgccgga caccgccggc tacacgcgcg tcagcgtgcg
     1441 cgccccggcc agcggcccgc tggtggccaa cgagccggtc gtgctcgtgc tcgccggcag
     1501 cgatgccgcc gtcaacctcg cgccggtgct gagcggccag aacaacccga gcaacggccg
     1561 ccggcgcgtg ctgcgcggca cgctgaacgc cgccggcacg gtgctgacgc tgacggcggc
     1621 cgacaaccgc gtcatggctg ccgaccagcc ggtcgcggag ctgtcgctgt cggccgatgc
     1681 gacgctcgcg ctcgccgcgc tcgcgacgct gccggatacc ggcgccgcgc tgttcgcgct
     1741 gcgtgccttc gacctcgacg tgctgtcggc ccccgacccg gtcgcgcccg agcgggcgcc
     1801 ggagccggtg ttcgcagtcg cggacctgtc gccgtcgccg caggccgtgc gccacctcgc
     1861 cgcgctgctg ccgatgcagc cggcgcggcg cgccgagcgg ctgacgcagc cggtcgcggc
     1921 gctgtacctc aatggcgaac cctcggccac gcaggtcatc gattcgatct tcgcgctcgc
     1981 cgacaccgtc gcgctgctgc cggcggccgg tgcgctggcc gggccgcgct ggctgatcca
     2041 catgagtggc ggcagcgacg gtccgctgcc gggggcggcc gactacaccg gcgagagcga
     2101 cgaactcacc ggctcgaccg gcttcgcggc gctcgaagag atcgaggaca tcgccatcgt
     2161 cgcgaccccg gccgccgccg cgcacccggc gagccatgcg caggtcgtgc aggcgctgtg
     2221 ggcgcactgc cggcgcatgc gctaccgcgt cggcatcgtc gatgccgagg ccggcatgag
     2281 cctcaacgag gtgcgcgcct tcgccggcca gttctccgac agcctgctgg cgctgtacta
     2341 cccgtgggtg gtcaccgccg acccgagcgg cgtgcgcccg gaactcaccg tgccgcccgg
     2401 cggcttcatc gccggcgtct acgccggcac cgacgtgcgc cgcggcgtgc acaaggcgcc
     2461 ggccaacgag gtgctgatcg gcgtcaccgg cctggagacc gacgtcaacc gcttccgtca
     2521 ggagctgctg aacccgaacg gcatcaactg cctgcgcttc ttcccggggc gcggctaccg
     2581 cgtctggggc gcgcgcacgc tcagcgacga tcccgaatgg cgctacgtca acgtccggcg
     2641 ctacttcctg ttcctcgaac gctcgatcga gaaatccacg cagtgggcgg tgttcgagcc
     2701 gaacggcgag gcgctgtggg cgaacgtgcg ctcgacggtc gaggacttcc tgtacgccga
     2761 atggacggcc ggccacctgc tcggcgcctc gaccaaggaa gcctacttcg tgcgctgcga
     2821 ccgctcgacg atgacgcaga acgacatcga caacggccgg ctggtctgcc tgatcggcgt
     2881 cgcgccgctg cgcccggccg aattcgtgat cttccgcatc ggccagaaga ccgccgacgc
     2941 ccgctgacag aggcccgccg accatgcccg tgctgcgcga caccccgtac accgcgttca
     3001 acttcctcgt cgagatcgag cccggcatcg gccgcgagct gcgtgccggt ttttccgatg
     3061 tctcggggct caacaacgag atcaccgtcg ccgagtaccg cgccggcaac tcgaaggtca
     3121 accacgtgca gaagcgcccc ggcgtctaca aggccggcga cgtgacgctc aagcgcggca
     3181 tcatcggcgc gaccgacctg tgggactgga tcgtcgcgac ccgcgagggc cgcgtcagcg
     3241 aggccaagcg caccgtcgtc gtcagcctgc tcgccgagaa ccgcgacgac gtggtcgtgc
     3301 gctggaaact ccagggtgcg atgccgatga agtggaccgg cccgacgctg gccggcaagg
     3361 gcggcggcga cgtggcgatc gaggaactcg tgctgtcggt cgagtcggtc gatcaggaat
     3421 aagccgccga tgctcacgtt tgccgatgcg cgcgtgttcc tgaacccggc gccggtcgcg
     3481 ccgcgccagc cggtgctgcc ggtggcgctg gtcggccacg tgccgggcgc ggcacgcccc
     3541 gaacggctgt cgcgcgcgct gcgcgagccc tggcacgggc tcggcctgca gcagcgcgag
     3601 cagcagctcg accatgcgct cgcaaacggc ccggccggcg acgacgacga tgccctgctc
     3661 gcgctgcatc acctgccgct cgcactcgac agccacgcgc agttcctcga cctctttccg
     3721 gacgccggca ctgccgcgac cggcccggcc gccccggtcg gcgccgcgcc ctggctgccg
     3781 ctcgcgatcg aggacttctt cgccgccggc ggcgagcggc tctggctggt gcgcgtgccg
     3841 cagagcgaag gccgcgacgg cctgctgccc gcgcagcccg gcgatccggc ccggcccgac
     3901 accctgcgcg gcctcgacgt ggcgctgaac gtgcccgaca tcggcctcgt cgcgctgccc
     3961 gacctcgaac gcctgcagct gccggccggc ctcgatccgc tgcccgattt cacgcccgca
     4021 ccggcgcagg cccgcttcgt gccggtcgac gaactcgcgc gtgccgatgc cgtgccgacg
     4081 ccgccgccat caccgctgtc gccaccgtgg ccgctcgccg aagtggtgcg ccggctctgc
     4141 gcgcgcttcg aggcctggcg cccggacgtg caggcactgc tgacgctgcc gctcgccgcg
     4201 accctcgacg gcggtgcgca gcccgatgcg gccgcgctcg cctggctcgc ggccctcgat
     4261 ggcgaggaac tgcgccgcgc gcagcgcctg cagccgctgt atccgtacct gcgcggccgg
     4321 cgcgcgccgc tcgcgagcgc gaccggcctc gtcgccggca cgatcgccgc gagtgccttc
     4381 cgcgacggcc cgtggcgctc ggtggccggg cgcgacctcg gcggcgacgc gctgccgtgg
     4441 ccggt
//
LOCUS       DAOQIE010000310         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11706_length_4403_cov_6.337856, whole genome shotgun sequence.
ACCESSION   DAOQIE010000310 DAOQIE010000000
VERSION     DAOQIE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11706_length_4403_cov_6.337856"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /gene="accB"
                     /locus_tag="PK144_15600"
     CDS             <1..257
                     /gene="accB"
                     /locus_tag="PK144_15600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004392389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNF67202.1"
                     /translation="AAPAAPAGHALKSPMVGTFYRAPAPGAKPFVEVGQHVSVGDVVC
                     VIEAMKMFNQIEADRAGTISAVLVESGQPVEFDQPLIVIE"
     gene            264..1610
                     /gene="accC"
                     /locus_tag="PK144_15605"
     CDS             264..1610
                     /gene="accC"
                     /locus_tag="PK144_15605"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNF67203.1"
                     /translation="MLDKVVIANRGEIALRILRACHELGIKTVAVYSTVDRELKHVRL
                     ADETVCIGPAPSAESYLNMVALISAAEVTDAVAIHPGYGFLSENADFAERVEQSGFIF
                     IGPRAETIRLMGDKVSAIRAMKAAGVPCVPGSDGPLGDDEQESVEIARRIGYPIIVKA
                     AGGGGGRGMRVVHTEASLLNAIALTRAEAQAAFGNPMVYMEKYLVHPRHVEIQVLADT
                     LGNAIFLGERDCSMQRRHQKVVEEAPAPGITPEQRMRIGSVCVEACRRIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTEMITGVDIVKEQLRIAAGLPLSFTQDDIRISGHA
                     VECRLNAEDPDTFLPSPGTITRYHAPGGPGVRVDSHLYAGYRVPPNYDSMIGKLIAHG
                     ETREVAIARMRNALAELVVDGIKTNAPLHRRIMADANFIEGGTDIHYLEAKLGLKS"
     gene            1660..2538
                     /gene="prmA"
                     /locus_tag="PK144_15610"
     CDS             1660..2538
                     /gene="prmA"
                     /locus_tag="PK144_15610"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNF67204.1"
                     /translation="MSWLQLTLEAGDHTVVQLEDALLEAGAVAVTLSDAVDEPVLEPA
                     PGETPLWRRTLVTGLFDAGTDVETAKHLVCAALGCDELPEARIGALEERDWVRAWMDH
                     FHPMRFGRRLWVVPSCREPPEPEAVNLLLDPGLAFGTGTHPTTALCLEWLDGVELAGR
                     SVIDYGCGSGILAIAALKLGAASAHGVDIDPQALHATRENAAVNGVDKHLRVGLPETL
                     PGQAADVVLANILAGPLLMLAPRLAAATRPGGHVVLAGLLERHADELEAAYGEWFEMQ
                     PRGLREGWVRLHGIRR"
     gene            2622..3611
                     /gene="dusB"
                     /locus_tag="PK144_15615"
     CDS             2622..3611
                     /gene="dusB"
                     /locus_tag="PK144_15615"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNF67205.1"
                     /translation="MQIGPHVLPSPLVLAPMAGVTDRPFRTLCRRLGAGLAVSEMVTS
                     DVRLWHTGKSRRRLDHAGEDGIRSVQIAGADPAQMAEAARQNVGRGAEIIDLNMGCPA
                     KKVCSVAAGSALLRDEALVARILGAVVAAVEVPVTLKIRTGWDRASRNGVTIARIAED
                     CGIRALAVHGRTRADAFAGEAEYDTIAAIKAAVRIPVIANGDIDGPEKARAVLEHTGA
                     DALMIGRAAQGRPWIFREIAHFLATGERLPEPGPAEVCALLLEHLEQLYTFYGEDAGV
                     RIARKHLGWYCGTRPGGAALRSLVNQTVSSREQLAAVRDLFDRLETQQDPLAA"
     gene            3608..3880
                     /gene="fis"
                     /locus_tag="PK144_15620"
     CDS             3608..3880
                     /gene="fis"
                     /locus_tag="PK144_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HNF67206.1"
                     /translation="MNPTDLDPPPAPAPAPAPLRAHVQAALHDYFRQLEGQMPTGLYD
                     LVLQEVERPLLETVMHFTHGNQTRAAEVLGINRSTLRKKLRQYGLE"
     gene            complement(4203..>4403)
                     /locus_tag="PK144_15625"
     CDS             complement(4203..>4403)
                     /locus_tag="PK144_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNF67207.1"
                     /translation="FCGAPGFQLSNGSTFLETHHVIPLSEGGVDSVCNVVALCPNHHR
                     EAHYGSNAEAIREHLLSVLSGT"
BASE COUNT          700 a         1601 c         1447 g          655 t
ORIGIN      
        1 ccgcggcccc ggctgcaccg gccggccatg cgctgaagtc gccgatggtc ggcaccttct
       61 atcgcgcccc ggccccgggc gcgaagccct tcgtcgaggt cggccagcat gtgtcggtcg
      121 gcgacgtggt ctgcgtgatc gaggcgatga agatgttcaa ccagatcgag gcggaccgcg
      181 ccggcaccat cagcgccgtc ctggtcgaga gcggtcagcc ggtcgaattc gaccagccgc
      241 tgatcgtcat cgagtaatca cgcatgctcg acaaggtcgt catcgccaac cgcggcgaga
      301 tcgcgctgcg catcctgcgc gcctgccacg aactcggcat caagaccgtc gcggtgtact
      361 cgaccgtcga tcgcgagctg aagcacgtgc gcctcgccga cgagaccgtc tgcatcggcc
      421 cggcgccctc ggccgagagc tacctgaaca tggtggcgct gatctcggca gccgaggtca
      481 ccgacgcggt cgcgatccac cccggctacg gtttcctgtc cgagaacgcc gacttcgccg
      541 agcgcgtcga gcagtccggc ttcatcttca tcggcccgcg cgccgagacc atccgcctga
      601 tgggcgacaa ggtctcggcg atccgcgcga tgaaggccgc cggcgtgccc tgcgtgcccg
      661 gctccgacgg cccgctcggc gacgacgagc aggaatcggt cgagatcgcc cggcgcatcg
      721 gctacccgat catcgtcaag gccgccggcg gcggtggcgg gcgcggcatg cgcgtcgtgc
      781 acaccgaggc ctcgctgctc aacgccatcg cgctgacccg cgccgaggcg caggccgcct
      841 tcggcaaccc gatggtgtac atggagaagt acctggtgca tccgcgccac gtcgagatcc
      901 aggtgctcgc cgacacgctc ggcaacgcca tcttcctcgg cgagcgcgac tgctcgatgc
      961 agcgccgtca ccagaaggtc gtcgaggaag ccccggcccc cggcatcacg cccgagcagc
     1021 gcatgcgcat cggcagcgtc tgcgtcgagg cctgcaggcg catcggctac cgcggcgccg
     1081 gcaccttcga gttcctgtac gagaacggcg agttctactt catcgagatg aacacgcgcg
     1141 tgcaggtcga gcatccggtc accgagatga tcaccggcgt cgacatcgtc aaggagcagc
     1201 tgcgcatcgc tgccggcctg ccgctgtcgt tcacgcagga cgacatccgc atcagcggcc
     1261 acgccgtcga atgccgcctc aacgccgagg acccggacac cttcctgccc tcgccgggca
     1321 cgatcacgcg ctaccacgcg ccgggcggtc cgggcgtgcg cgtcgattcg cacctgtacg
     1381 ccggctaccg cgtgccgccg aactacgact cgatgatcgg caagctgatc gcgcacggcg
     1441 agacccgcga ggtcgcgatc gcgcgcatgc gcaatgcgct ggccgagctc gtcgtcgacg
     1501 gcatcaagac caacgcgccg ctgcaccgcc ggatcatggc cgatgccaac ttcatcgaag
     1561 gcggcaccga catccactat ctggaagcca agctcggtct caagagctga aacccgcggg
     1621 aggtccgcca ccgtgtgcga ggacgaggag gatgacgccc tgagctggct gcaactgacg
     1681 ctcgaagccg gtgaccacac ggtcgtgcag ctcgaagacg cgctgctcga agccggcgcc
     1741 gtggccgtga cgctttccga tgccgtcgac gagcccgtgc tcgaaccggc ccccggcgaa
     1801 acgccgctgt ggcggcgcac gctggtcacc ggcctgttcg acgccggcac cgatgtcgag
     1861 accgccaagc acctggtctg cgccgccctc ggctgcgacg agctgcccga ggcgcgcatc
     1921 ggtgcgctcg aagaacgcga ctgggtgcgg gcatggatgg atcacttcca tccgatgcgc
     1981 ttcggccggc ggctgtgggt ggtgccgagc tgccgcgagc cgcccgagcc cgaggccgtg
     2041 aacctgctgc tcgatccggg cctcgccttc ggcaccggca cgcatccgac cacggcgctc
     2101 tgcctcgaat ggctggacgg cgtcgaactc gccgggcgtt cggtcatcga ctacggctgc
     2161 ggctccggca tcctcgcgat cgccgcgctg aagctcggtg ccgccagcgc ccacggcgtc
     2221 gacatcgacc cgcaggcgct gcacgcgacg cgcgagaacg ccgccgtcaa cggcgtcgac
     2281 aagcacctgc gcgtcggcct gcccgaaacc ctgcccggcc aggccgccga tgtcgtgctc
     2341 gccaacatcc tcgccggccc gctgctgatg ctcgcgccgc ggctcgccgc cgccacccgg
     2401 cccggcggcc acgtcgtgct cgccggcctg ctcgaacgcc acgccgatga gctcgaagcc
     2461 gcctacggcg aatggttcga gatgcagccg cgcgggctgc gcgaaggctg ggtgcggctg
     2521 cacggcatcc gccgctagcg gccgcgctgc ggccggcccg tcgaatccgc cccgcaccgg
     2581 cccggggcaa gctgctccgc cccccccgag gttccgctgc cgtgcagatc ggcccgcatg
     2641 tgcttccctc cccgctcgtc ctcgcgccga tggccggcgt caccgaccgg ccgtttcgca
     2701 cgctctgccg ccggctcggc gccgggctcg cggtttcgga gatggtcacg tccgacgtgc
     2761 gcctgtggca caccggcaaa tcccgccgcc ggctcgatca cgccggcgag gacggcatcc
     2821 gcagcgtgca gatcgccggt gccgacccgg cgcagatggc cgaggccgcg cgccagaacg
     2881 tcgggcgcgg ggccgagatc atcgacctca acatgggctg cccggcgaag aaggtctgct
     2941 cggtggccgc cggctcggcg ctgctgcgcg acgaggcgct ggtcgcgcgc atcctcggcg
     3001 ccgtggtcgc ggcggtcgag gtgccggtga cgctgaagat ccgcaccggc tgggaccgcg
     3061 ccagccgcaa cggcgtgacg atcgcccgca tcgccgagga ctgcggcatc cgcgccctcg
     3121 ccgtgcacgg gcgcacgcgc gccgatgcct ttgccggcga ggccgagtac gacaccatcg
     3181 ccgcgatcaa ggccgccgtg cgcatcccgg tgatcgccaa cggcgacatc gacggccccg
     3241 agaaggcccg cgccgtgctc gaacacaccg gcgccgatgc gctgatgatc ggtcgcgccg
     3301 cgcagggccg gccgtggatc ttccgcgaga tcgcgcattt cctcgccacc ggcgagcgcc
     3361 tgcccgaacc ggggccggcc gaggtgtgcg cgctgctgct cgaacacctc gaacagctct
     3421 atacctttta cggcgaggac gccggcgtgc gcatcgcccg caagcatctc ggctggtact
     3481 gcggcacccg ccccggcggc gcggcgctgc gcagcctcgt caaccagacg gtcagcagcc
     3541 gggaacagct cgcggccgta cgtgatctgt tcgaccgcct cgaaacacaa caggacccgc
     3601 tggccgcatg aaccccaccg acctcgaccc gcccccggct cccgccccgg cccctgcccc
     3661 gctgcgcgcc cacgtacagg ccgcgctgca cgactacttc cgccagctcg aaggccagat
     3721 gccgaccggg ctgtacgacc tcgtcctgca ggaagtcgag cgcccgctgc tcgaaaccgt
     3781 catgcacttc acgcacggca accagacccg ggccgccgaa gtgctcggca tcaatcgcag
     3841 tacgttgagg aagaagttgc ggcagtacgg gctggagtag gcggcaggcg gtaaacgctt
     3901 gccgggatac ttcgcacgat gggcgtgcag cgtgatcaat atgagcggcg cgatattcgt
     3961 ttgaccggca ggctgggtcc gtccagggct ttcaaggtgt cggaatgggt ctgtctgtaa
     4021 ctactggatc tgctttggtc ggatcagact tgtaggatgt gccgacgcag gaggcgcatc
     4081 gttcgcggaa ttcgagcagc gattaccgta ccttccagcg cattacgcga acgatgcgct
     4141 tcgttcctca gcgcatccta cgggcttttc gcgcaggtcc ggttgagggt tcagcgtcat
     4201 tgtcacgtac cgctcaagac actcaacaaa tgttcgcgaa tagcttcggc gttactgccg
     4261 taatgcgcct cgcggtgatg gtttgggcag agggcgacaa cattacatac gctgtcgaca
     4321 ccgccctcgg acagcggaat tacgtgatga gtttccaaga acgtgctgcc gttggaaagt
     4381 tgaaatcccg gtgcaccgca gaa
//
LOCUS       DAOQIE010000311         4362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11864_length_4362_cov_7.402368, whole genome shotgun sequence.
ACCESSION   DAOQIE010000311 DAOQIE010000000
VERSION     DAOQIE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11864_length_4362_cov_7.402368"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..733
                     /locus_tag="PK144_15630"
     CDS             464..733
                     /locus_tag="PK144_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67208.1"
                     /translation="MDDGMPSRRSSTGFMMQQRLRGAKNSAWISLTGPDDRPETLRLR
                     MALMLSSAARRKTDPYQLADASLLQRKNDDAPQGTGRQLFFTDFL"
     gene            complement(746..1630)
                     /gene="galU"
                     /locus_tag="PK144_15635"
     CDS             complement(746..1630)
                     /gene="galU"
                     /locus_tag="PK144_15635"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNF67209.1"
                     /translation="MKRKIRKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAVE
                     EAVAAGVDTLVFVIGRTKNAIADHFDKAYELETELETRGKKDLLKVVQSIVPPHVSCV
                     YVRQAEALGLGHAVLCAKPIVGQEPCAVLLADDLIDAPRGGAMAQMAAHFDKYQCSIL
                     GVERVPPEETFKYGIVTPLPFAAHLSNVDSIVEKPKPEEAPSNLAVVGRYILTPRIFE
                     LLEKTPRGAGNEIQLTDAIAALLLEEQVLAYEFEGRRYDCGSKIGYLTATVEYGMKHA
                     ELGAQFRQYLQSMVCSIG"
     gene            complement(1813..3159)
                     /gene="metC"
                     /locus_tag="PK144_15640"
     CDS             complement(1813..3159)
                     /gene="metC"
                     /locus_tag="PK144_15640"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HNF67210.1"
                     /translation="MQDDTRLIHHGRAGAGAGYDGLVNPPVCHASTILFPTLDALEGR
                     TPVPMSYGRHGTPTQFALAEALSALHGGAGTVLVPSGLAAAVLAISTFAHVGTHVLIT
                     DSVYGPTRAYCDELARHRGVTVEYFDPLIGAGIEALLRPETALVFLESPGSLTFEIQD
                     VPAIAAVAHARGVHVAIDDTWSAGYFMKPLALGADISVQALTKYVGGHSDLMLGAVIC
                     NAGTLDAVKHQARLTGNASAPDDVSLALRGLRTLAVRLHQHELSGLDLARWLVQQPQV
                     ARVVHPGRPDHPQHDLWLRDFSGACGLFGFELHPCTRTQLAAFLDGLKLFGMGYSWGG
                     FESLIIPAHAPRTVRKWKTAGPWLRVHVGLEEIEDLINDLALGFQRLSAAAPPPGPAA
                     PAAAETAVAAAGSEPAAPGSDGPPDSDSEELPPAEMTEPAGEQPAAGDPPLPPAVA"
     gene            3285..4277
                     /locus_tag="PK144_15645"
     CDS             3285..4277
                     /locus_tag="PK144_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67211.1"
                     /translation="MSVRRCLLLCLLLPCLLSASGLRTAAAGSDDSPVVRSTSVGSGP
                     QLLARIVDGAQDFDEWHELEIELSVATDGSRYLVRRLRVLPSDEVEPECSDIQRMGRL
                     VLPAQATTVLPPLELEVSERQRNAWLKRLLQRQHRRYVVLALELQRLDAAPASSVASV
                     APLLAPATLSAAQARCPDAFGPDDQQYEWLFATLPVLIDTPPWSMHVGAVLGAVLSTL
                     FVFLSARKGETADTASGGERRPRPQDLPGELLLTSLACLIAVMLLQSTSSAAAGEAGL
                     PLQVAVKDFYGGLLIGLFGRQIVGFVRRRLSSDPGTMPAPADTPATAAPDQMYW"
BASE COUNT          656 a         1493 c         1529 g          684 t
ORIGIN      
        1 cgcggatggt gcgggcgacg gcgtaggcga tcgaactctc gttggcgatg ccgacgacca
       61 ggccgcgctt gccgtgcagg ctgaagggat tgctcatgat ggctgactcc agggggaagg
      121 gccggatggt gacggtcacg gctcggccgg gacccgacac acgtggggaa aatcgacgct
      181 ggacctgctg ccgctgcaca tgcctgacct ttgattttta tagtgcggtg cagcatcggc
      241 cgggacgagt atatccctgc cggcaggctc ggccagcgaa tcaaaacctc caggggggtt
      301 gcgccaaagc gcggatcagc cggtgagggc cgggccggtc ctgcggtcgg ggcgggaaga
      361 acggcttccg gagccgggcc gtgtacggca aaccaggttt acggtcaccg cgacccgggg
      421 gtcggaatgg cgggccggca gcgtccgctg cgactcgcgg gggatggacg acgggatgcc
      481 gtcgcgcagg tcatcaacag gcttcatgat gcagcagcgc ctccggggcg cgaaaaactc
      541 cgcgtggatc agtctgacgg ggccggatga ccgccctgag accctccggt tgcggatggc
      601 ccttatgctg tcatcggccg cccggcggaa aactgatccg tatcagcttg cggacgcttc
      661 gctgctgcag cgcaaaaacg acgacgcccc gcagggcacg gggcgtcagc ttttctttac
      721 agattttttg tgagccgggc agcgctcagc cgatgctgca gaccatcgac tgcaaatact
      781 ggcggaactg cgcgccgagt tcggcgtgct tcatgccgta ttcgacggtc gcggtcagat
      841 agccgatctt gctgccgcag tcatagcgcc ggccttcgaa ttcgtaggcc agcacctgct
      901 cttcgagcag cagcgccgcg atcgcgtcgg tgagctggat ctcgttgccg gcaccgcgcg
      961 gggtcttttc gagcagctcg aagatgcgcg gcgtcaggat gtagcgaccg accaccgcca
     1021 ggttcgacgg cgcctcctcg ggcttcggct tctcgacgat gctgtcgacg ttcgacaggt
     1081 gcgcggcaaa cggcagcggc gtgacgatgc cgtacttgaa ggtttcttcg ggcggcacgc
     1141 gctcgacgcc gaggatcgag cactggtact tgtcgaagtg cgccgccatc tgcgccatcg
     1201 ccccgccgcg cggtgcgtcg atcaggtcat cggcgagcag caccgcgcac ggctcctgac
     1261 cgacgatcgg cttggcgcag agcaccgcgt gaccgaggcc gagcgcttcg gcctgacgca
     1321 cgtacacgca ggagacgtgc ggcggcacga tgctctgcac gaccttcagc agatccttct
     1381 tgccgcgggt ttcgagttcg gtctcgagct cgtaggcctt gtcgaaatga tcggcgatcg
     1441 cgttcttggt gcggccgatg acgaacacca gcgtatcgac gccggccgca accgcttcct
     1501 cgaccgcgta ctggatcagc ggcttgtcga cgatcggcag catctccttc gggctggcct
     1561 tggtggccgg caggaagcgg gtgccgaggc cggcgacggg gaacacggcc ttgcgaatct
     1621 tgcgcttcac gttcggggtt cctcgctctg tcttctgctg gatagggctg aatagggctg
     1681 gacatgaccg gacatcgcgg gttccggcct gaaatgatcg cggtgatccg tggagcggcg
     1741 gcattctgcg cgccgcccac agacccgaca agcgaacgcc ggcgcgatgg tacgaagatt
     1801 gacgcagcgg tttcaggcca ccgccggcgg caacggcgga tcaccggctg caggctgctc
     1861 gcctgcgggt tcggtcatct ccgccggcgg aagttcttcg ctgtcgctgt ccggcgggcc
     1921 atcgctgccg ggcgcagccg gctcgctgcc ggcggcagcc actgcggttt cggccgctgc
     1981 cggcgcagcg ggtccgggag gcggggcggc ggcgctcagg cgctggaagc cgagcgcaag
     2041 atcgttgatc aggtcctcga tctcctccag cccgacgtgc acgcgcagcc atggccccgc
     2101 cgtcttccac ttgcgcaccg tgcgcggcgc gtgcgccgga atgatcaggc tctcgaagcc
     2161 gccccacgaa tagcccatgc cgaacagctt caggccatcc aggaaggccg cgagctgcgt
     2221 gcgcgtgcac ggatgcagct cgaagccgaa caggccgcag gcaccgctga agtcgcgcag
     2281 ccagagatcg tgctgcggat ggtcgggccg acccggatgc acgacgcgcg cgacctgcgg
     2341 ctgctgcacc agccagcgcg cgaggtccag accgctcagc tcgtgctggt gcaggcgcac
     2401 ggccagcgtg cgcaggccgc gcagcgccag cgacacgtca tcgggcgcgc tggcgttgcc
     2461 ggtcaggcgc gcctgatgct tcacggcatc gagcgtcccg gcgttgcaga tcacggcgcc
     2521 gagcatcagg tcggagtgac cgccgacgta cttggtcagc gcctgcaccg agatgtccgc
     2581 cccgagcgcg agcggcttca tgaagtagcc ggcgctccag gtatcgtcga tcgcgacatg
     2641 cacgccgcgg gcatgggcaa cggcggcgat cgccggcacg tcctggatct cgaaggtcag
     2701 cgaaccgggg gattcgagga acaccagcgc cgtttccggc ctcagcagcg cctcgatgcc
     2761 ggccccgatc agcggatcga agtactcgac cgtcacgccc cggtggcgcg cgagttcgtc
     2821 gcagtaggcg cgggtcggac cgtagacgct gtcggtgatc agcacgtgcg tgccgacgtg
     2881 cgcgaaggtc gagattgcca gcaccgccgc cgcgaggccc gagggcacca gcaccgtgcc
     2941 ggcgccgccg tgcagcgcgc tcagcgcctc ggccagcgcg aactgcgtcg gcgtgccgtg
     3001 gcggccgtag ctcatcggca ccggcgtgcg gccttcgagc gcatcgaggg tcgggaacag
     3061 gatggtcgag gcgtggcaga ccgggggatt gacgagtccg tcatacccgg ccccggctcc
     3121 ggcccgcccg tgatggatca gtcgggtatc gtcctgcatg gcgccctccg gcggctcgac
     3181 gaggccggcg agcctagcac gcccgctccc tgcggccgga cacaagttgc cataatcgcc
     3241 acagccgatc cccgctgcgc gcccgcttgc gcgaaggaac gccgatgtcc gtccgccgct
     3301 gcctgctcct ctgcctgctc ctgccctgcc tgctgtcggc cagcggcctg cgcaccgccg
     3361 cagccggcag cgatgattcg ccggtcgtgc gctcgacctc ggtcggcagc gggccgcagc
     3421 tgctcgcccg catcgtcgac ggtgcgcagg acttcgacga atggcacgag ctggaaatcg
     3481 agctgagcgt cgccaccgac gggtcgcgtt acctggtgcg ccggctgcgc gtgctgccga
     3541 gcgacgaggt cgaaccggaa tgctcggaca tccagcgcat gggccggctg gtgctgccgg
     3601 cgcaggcgac gacggtgctg ccgccgctgg agctggaagt ctcggagcgc cagcgcaacg
     3661 cctggctgaa gcgcctgctg cagcgccagc accggcgcta cgtcgtgctc gcgctcgaac
     3721 tgcagcgcct cgacgccgcg cccgccagca gcgtcgccag cgtcgcgcca ctgctcgccc
     3781 cggccacact cagtgccgcg caggcgcgct gtccggacgc cttcgggccg gacgaccagc
     3841 agtacgaatg gctgttcgcg acgctgccgg tgctgatcga cacgccgccg tggtcgatgc
     3901 acgtcggggc cgtgctcggc gccgtgctct cgacgctgtt cgtgttcctg agcgcgcgca
     3961 agggcgagac cgccgacacc gccagcggcg gcgaacgccg gccccggccg caggacctgc
     4021 ccggcgaact gctgctgacc tcgctcgcct gcctgatcgc cgtgatgctg ctgcagtcga
     4081 ccagttcggc ggcggccggc gaggccgggc tgccgctgca ggtcgcggtc aaggatttct
     4141 acggcggact gctgatcggt ctcttcgggc gccagatcgt cggcttcgtg cgccggcgcc
     4201 tcagcagcga ccccggcacg atgccggccc cggcggacac ccccgccacc gccgcgccgg
     4261 accagatgta ctggtagggc ttcgtcaggt ccgatccgag ccactggccg ttgccgtaga
     4321 aatccacccg ggccaccgtt ccgtcgacat cgctcgcact gg
//
LOCUS       DAOQIE010000312         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11906_length_4355_cov_7.120465, whole genome shotgun sequence.
ACCESSION   DAOQIE010000312 DAOQIE010000000
VERSION     DAOQIE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11906_length_4355_cov_7.120465"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK144_15650"
     CDS             complement(<1..511)
                     /locus_tag="PK144_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNF67212.1"
                     /translation="MTSSADLRLAFLEYFRARGHEVVPSSPLIPGNDPTLLFTNAGMV
                     QFKDVFLGTDPRPYVRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYF
                     KRDAIRFAWEFLTTVLRLPAEKLWVTVYASDDEAHDLWAGEIGVPRERIVRIGDNKGA
                     PYASDNFWQM"
     gene            complement(552..1043)
                     /locus_tag="PK144_15655"
     CDS             complement(552..1043)
                     /locus_tag="PK144_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNF67213.1"
                     /translation="MSSDEPGDPDDPAEAGALLYRTALALLARREHTRLELAAKLARH
                     GAGAGIIAAVLDRLQAEQLLSEQRFAEVYAATRFDRGYGPLRLRAELRERGVSEAIVS
                     DILAGFDAQWTARLAALARRRFGPQPPKDARERAQRTAFLRGRGFTGEQIARLMRRDP
                     GDD"
     gene            complement(1033..2088)
                     /gene="recA"
                     /locus_tag="PK144_15660"
     CDS             complement(1033..2088)
                     /gene="recA"
                     /locus_tag="PK144_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNF67214.1"
                     /translation="MDDNRRKALAAALGQIEKQFGKGSVMRLGDANAARDIDVISTGS
                     LGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKLGGTCAFVDAEHALDPI
                     YAAKLGVKVADLLVSQPDTGEQALEITDMLVRSAAVDVVVVDSVAALTPKAEIEGEMG
                     DSHVGLHARLMSQALRKLTGNINRSNTLVIFINQIRLKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRTGSIKKGDEVLGSETRVKVVKNKVAPPFKQAEFEILYGEGISRLGEIID
                     LGVKAALIDKSGAWYSYGGQRIGQGKENVRQFLKDNPAIAAELDAKVRAHYLSNPDAL
                     PTVPVGDDEAGGTGDEF"
     gene            complement(2169..2699)
                     /gene="thpR"
                     /locus_tag="PK144_15665"
     CDS             complement(2169..2699)
                     /gene="thpR"
                     /locus_tag="PK144_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNF67215.1"
                     /translation="MTQAPAPGRRLFLALWPDPAVRAELARLARRLTPEHVPAANLHL
                     TLVFLGFTPPEREAGCRAALEGLRPAPFTLELDRLGCFRGPRVLWLGPSQIPPALTAL
                     VGELQRRLAACAHEPDPRPFAAHVTLARRFSGSAPAYPLPSPIRWPAARIVLAESRSQ
                     PGGVRYEPLADWPVGL"
     gene            complement(2696..3199)
                     /locus_tag="PK144_15670"
     CDS             complement(2696..3199)
                     /locus_tag="PK144_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNF67216.1"
                     /translation="MNATDPDPYARARAVGQALAARGLRLACAESCTGGGIAHALTAV
                     PGSSAWFERGFVTYSNAAKEQLLGVQPATLAAHGAVSAETVIEMALGTLAHAEAEFAV
                     AVSGIAGPDGGSADKPVGTVWIAWARAGREPQARRFVFAGDRAMVRAAAVLAALDGVL
                     ERLDGDP"
     gene            complement(3196..3678)
                     /locus_tag="PK144_15675"
     CDS             complement(3196..3678)
                     /locus_tag="PK144_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67217.1"
                     /translation="MSALVLLAAVTAPAAELDDIRRLADEGQQDAALRRLDERLVRDP
                     SDVPARFLKGSILLQTGKLGEAREAFDEVTRRAPKLPEPYNNLAVIYAAQGDYEKARQ
                     SLYTAAQQAPDYAAAQINLGDLYVKLAVDAYRRALELNPADEASRAKLLALDAMFRRQ
                     "
     gene            complement(3841..>4355)
                     /locus_tag="PK144_15680"
     CDS             complement(3841..>4355)
                     /locus_tag="PK144_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67218.1"
                     /translation="GTRTLVDNLTAGIIPYQSSTRVDATVGQLIINNGVGEVTGSSVT
                     WALPANLLPTTGSLSNFQFHMALTGKDNNAVSVSVLLNGSTIGTSSLPTAQVTTLQDL
                     YFSSTIASFNAGGTLQLVFSGNAGWDASFDTFGFQYDYTPPPPVTVPVPATLALFGIG
                     AVALHRRRRC"
BASE COUNT          655 a         1477 c         1578 g          645 t
ORIGIN      
        1 ccatctgcca gaagttgtcg gaggcatacg gcgcgccctt gttgtcgccg atgcgcacga
       61 tgcgctcgcg cggcacgccg atctcgccgg cccagagatc gtgggcctcg tcgtcgctgg
      121 cgtagacggt gacccagagc ttttcggccg gcaggcgcag caccgtggtc aggaactccc
      181 acgcgaagcg gatcgcatcg cgcttgaagt agtccccgaa gctgaagttg ccgagcatct
      241 cgaagaaggt gtggtggcgc gccgtgtagc cgacgttctc gagatcgttg tgcttgccgc
      301 cggcgcgcac gcagcgctgc gcggtggtgg cccggacata cggacgcggg tcggtgccga
      361 ggaacacgtc cttgaactgc accatgccgg cgttcgtgaa cagcagcgtc ggatcgttgc
      421 cgggaatcag cggactcgac ggcacgacct cgtgcccgcg ggcgcggaag tactccagga
      481 aggcgaggcg taggtcggcg ctgctggtca tggtcggctg caaactgggt cagggttgcg
      541 gaggtgggcg ctcagtcgtc tccgggatcg cggcgcatca gccgggcgat ctgctcgccg
      601 gtgaaaccgc gaccgcgcag gaacgcggtg cgctgcgccc gttcgcgggc atccttcggc
      661 ggctgcggac cgaagcggcg gcgcgcgagg gctgccagcc gcgccgtcca ctgcgcgtcg
      721 aaacccgcga ggatatcgga aacaatcgct tccgacacgc cccgttcgcg cagctcggcg
      781 cgcagccgca gcggaccgta gccgcgatcg aagcgcgtgg cggcgtagac ctcggcgaaa
      841 cgctgctcgc tgagcagctg ctcggcctgc agccggtcga gcaccgccgc aatgatgccg
      901 gccccggcgc cgtgtcgcgc aagcttggcg gcgagttcga gccgcgtgtg ctcgcgtcgc
      961 gccagcagcg ccagcgcggt gcgatacagc agcgcgccgg cctccgcggg atcgtccggg
     1021 tcgccgggct cgtcagaact catcgcccgt gccgccggcc tcgtcgtcac cgaccggcac
     1081 ggtcggcagc gcgtcggggt tgctcaggta atgcgcacgc accttggcgt cgagttcggc
     1141 ggcgatcgcc gggttgtcct tcaggaactg gcggacgttc tccttgccct ggccgatgcg
     1201 ctggccgccg tagctgtacc aggcgccgga cttgtcgatc agcgccgcct tgacgccgag
     1261 atcgatgatc tcgccgaggc gcgagatgcc ctcgccgtag aggatctcga actcggcctg
     1321 cttgaacggc ggcgcgacct tgttcttgac caccttgacg cgggtctcgg agccgagcac
     1381 ctcgtcgccc ttcttgatcg agccggtgcg gcggatgtcg aggcgcaccg aggcgtagaa
     1441 cttcagcgcg ttgccgccgg tggtggtctc ggggttgccg aacatcacgc cgatcttcag
     1501 gcggatctgg ttgatgaaga tcaccagcgt gttcgagcgg ttgatgttgc cggtgagctt
     1561 gcgcagcgcc tggctcatca gccgcgcgtg caggccgacg tggctgtcgc ccatctcgcc
     1621 ctcgatctcg gccttcggcg tcagcgccgc gaccgaatcg acgacgacga catcgaccgc
     1681 cgccgagcgc accagcatgt cggtgatctc cagcgcctgc tcgccggtgt ccggctgcga
     1741 gaccagcagg tcggcgacct tgacgccgag cttggcggcg tagatcggat cgagcgcgtg
     1801 ctcggcatcg acgaaggcgc aggtgccgcc gagcttctgg gcctcggcga tgacctgcag
     1861 cgtcagcgtg gtcttgcccg aggattccgg cccgtagatc tcgacgacac gcccgcgcgg
     1921 caggccgccg acgccgagcg cgatgtcgag gccgagcgag ccggtggaga tcacatcgat
     1981 gtcgcgcgcg gcattggcgt cgccgaggcg catcaccgag cccttgccga actgcttctc
     2041 gatctggcca agcgcagcgg cgagcgcctt cctgcggtta tcgtccattg cgtactcccg
     2101 gggaacggtg ggaacggatg tcgaacggcg caaggtgcgt ggagtattcc acaccacttg
     2161 cgggggacct agagcccgac cggccagtcg gccagcggtt cgtagcgcac gccgccgggc
     2221 tggctgcggg attcggcgag cacgatgcgc gcagccggcc agcggatcgg cgatggcagc
     2281 ggatatgcgg gtgcagaccc ggaaaatcga cgggcgagcg tcacgtgcgc ggcaaacggc
     2341 cggggatcgg gctcatgagc gcaggccgcg agccggcgct gcagttcccc gaccagcgcc
     2401 gtcagcgccg gcggtatctg cgacggaccg agccacagca cgcgcgggcc ccgaaaacag
     2461 ccgagccggt cgagttcgag cgtgaacggc gcaggcctca ggccctcgag tgcggcccgg
     2521 cagccggctt cgcgctccgg cggcgtgaag ccgaggaaga ccagcgtcag atgcaggttc
     2581 gccgccggca cgtgttccgg cgtcagccgg cgcgcaagtc gtgccagctc cgcgcgcacg
     2641 gccgggtccg gccacagtgc gaggaacagg cgccggccgg gcgcaggtgc ctgcgtcacg
     2701 gatcgccatc cagtcgttcg agcacgccat cgagcgccgc cagcaccgcc gccgcgcgca
     2761 ccatggcccg gtcgcccgcg aacacgaagc gcctggcctg cggctcgcgc ccggctcgcg
     2821 cccaggcgat ccagaccgtg ccgaccggct tgtcggcgct gccgccgtcc gggccggcga
     2881 tgccggagac cgcgacggcg aactcggcct cggcgtgcgc gagcgtgccg agcgccatct
     2941 cgatgacggt ctcggcgctg acggcgccgt gcgcggccag cgtcgccggc tgcacgccca
     3001 gcagctgctc cttggcggcg ttcgaatagg tgacgaagcc gcgctcgaac cacgccgagg
     3061 agccgggcac tgcggtcagc gcgtgggcga tgccgccgcc ggtacaggat tcggcacagg
     3121 ccagccgcag gccgcgggcc gcgagcgcct gaccgacggc ccgggcgcgt gcgtacggat
     3181 cgggatccgt cgcgttcact ggcgccggaa catcgcgtcg agcgccagca gcttggcgcg
     3241 cgaggcctca tcggccgggt tgagttccag cgcccggcgg taggcatcga cggcgagctt
     3301 cacgtacagg tcgccgaggt tgatctgcgc ggccgcgtag tccggcgcct gctgcgcagc
     3361 cgtgtacagc gactggcggg ccttctcgta gtcgccctgc gcggcgtaga tcacggccag
     3421 attgttgtac ggctccggca gcttcggcgc gcggcgcgtg acttcgtcga aggcctcgcg
     3481 cgcttcgccg agcttgccgg tctgcagcag gatcgagccc ttcaggaagc gcgccggcac
     3541 gtcggatgga tcgcgcacga ggcgttcatc gagccggcgc agcgccgcgt cctgctggcc
     3601 ttcgtcggcc agacggcgga tgtcgtcgag ttcggccgcc ggcgcggtga cggcagcgag
     3661 caggacgagc gcagacacaa atacgggaag ccggaagcgc atgcaggacg ttccttcgag
     3721 cggtcggacg gtgcgctgca tcctacaaca cgtgcccggc cgcaacccgc cccggaaacg
     3781 ccgcgccccc cgcgggcgga gcctcggggg gcgcggacag gcaggccgct cgcagcgcgg
     3841 tcagcagcgg cggcgacggt gcaacgccac agcaccgatt ccgaacagcg ccagcgttgc
     3901 cggcaccggc acggtgaccg gcggcggcgg cgtgtagtcg tactgaaagc cgaaggtgtc
     3961 gaaggaggca tcccagccgg cgttgccgct gaacaccagc tgcagcgtgc caccggcatt
     4021 gaagctggcg atggtggagg agaagtacag atcctgcagc gtggtgacct gagcagtcgg
     4081 cagcgaactc gtgccgatcg tcgaaccgtt cagcagcacg ctgaccgaaa ctgcattgtt
     4141 gtccttgccg gtcagcgcca tgtggaactg gaagttgctg aggctgccgg tcgtcggcag
     4201 caggttggcc ggcaatgccc aggtcacgct gctgccggtc acctcgccga cgccgttgtt
     4261 gatgatcagc tggccgaccg tggcatcgac gcgcgtcgag ctctgatacg gaatgatgcc
     4321 ggccgtcagg ttgtcgacga gcgtgcgcgt accgc
//
LOCUS       DAOQIE010000313         4355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_11907_length_4355_cov_5.863023, whole genome shotgun sequence.
ACCESSION   DAOQIE010000313 DAOQIE010000000
VERSION     DAOQIE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11907_length_4355_cov_5.863023"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK144_15685"
     CDS             complement(<1..553)
                     /locus_tag="PK144_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67219.1"
                     /translation="MRQKRRTPQRRAGGCGVRGCDGRDQRCAVVVGCFASFVFFASFA
                     FFTSAGFTGAAVAFAGAVVPVAFTGATFTGATFAGVAFTGVTFAGGVVREAAGLAGAA
                     RATTTGFAAGAGGLPATVAGFAAALPAVTGATAAGFAADASAGAFTAGAAAVAGFATT
                     SAARLPGGAIVTLWRAAGASACTG"
     gene            672..1286
                     /locus_tag="PK144_15690"
     CDS             672..1286
                     /locus_tag="PK144_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNF67220.1"
                     /translation="MLYAFDLFGVFVFAVSGALVAARKQLDWLGALVLAAVTAVGGGT
                     LRDLLLARHPLVWFSDAHYLLTIALAVAATWVWGRQRPVPKRALLVADAAGLSLFTVV
                     GTQVALTQGQGAVIAVLLGITTGAAGGAIRDVLCAEVPLVLRQEIYATAAAAGGLLFV
                     LLTGLGLPAAAAALVAMAAVFALRLAAIYRDWHLPRYRLRAEGE"
     gene            complement(1303..1914)
                     /locus_tag="PK144_15695"
     CDS             complement(1303..1914)
                     /locus_tag="PK144_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67221.1"
                     /translation="MSSTSAAAEPPRWVAGLLDSRSGAPAFYPRPGLRALLAELLHGD
                     GAQLVTARTGADGTPDLDHVAVTTLLQLDGSVLVQIDERSLAAPDWPAAFAVHRAALG
                     IAGERLSAYGKSVATTLTSIRGIAMCTLPALASPTPMGSRVAATVCAWWGFSPETAPL
                     LATGLHVLAQAVMPMAAGFGIARLAGPWLVRALLRLGMWWVRR"
     gene            complement(1889..>2613)
                     /locus_tag="PK144_15700"
     CDS             complement(1889..>2613)
                     /locus_tag="PK144_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67222.1"
                     /translation="HADDDSVAAVHIDAVLQDAAAPAEPPAPPPPASEHDTAIGRALA
                     LIAQTFPGLDAPYGHLADAIDALHPPGNAAAFESGAGPFDERFAGALLWATLFGSCAD
                     VAATLLAQTPAESPARAPLRRIARESRWLQAFIGWQHLQTTPGRGFDLQPEDRMRLRK
                     LWNLGGERVVMQTTIHLDGDVLNGIDPALLLHPQFEAVKALHADGVGTALTHWRALFE
                     AICTAFGKLGDAFSNVLDKRRG"
     assembly_gap    2614..2713
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2714..2991)
                     /locus_tag="PK144_15705"
     CDS             complement(<2714..2991)
                     /locus_tag="PK144_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67223.1"
                     /translation="MADLRQIALSIAQDLMTLEVNTVLKPGMTARKPPAYPLLLREIA
                     EKYVATLVQLDAGPAPIPGNPTADTVHIDAVHADDDSVAAVHIDAVLQ"
     gene            complement(2998..3339)
                     /locus_tag="PK144_15710"
     CDS             complement(2998..3339)
                     /locus_tag="PK144_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67224.1"
                     /translation="MADELDAGRGKLLEKLNAAFERMTTLDITTAVGEATKNPATGRY
                     EPAAGSKLLYTSIDLVGGDILNIVPADFGEGADDALRAFHQSQVASAQATVKANLDAV
                     EKLIALATGKD"
     gene            complement(3435..>4355)
                     /locus_tag="PK144_15715"
     CDS             complement(3435..>4355)
                     /locus_tag="PK144_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNF67225.1"
                     /translation="GGTKIELLALDADGRECLRRRVPTPQGDYAATLQAIRALVEAAE
                     QELGGRASVGLGTPGSASPRDGRMRNANSTCLNGRPLARDLEALLNRPLRFANDANCL
                     ALSEAVDGAAAGAGLVFAVILGTGVGGGLAAHGRVLNGHNGIAGEWGHNPLPWPAIGE
                     HPGPLCWCGRSGCIETWLSGPGFARTHAREHGLQQLVPAETIVGRARAGDPAAATSLA
                     RYCERLARALATVVNLLDPEVIVLGGGLSRVDELYARVPALMAPHVFADAVDTPLRPA
                     LHGDSSGVRGAAWLWNDGAAVSACQAGRGD"
BASE COUNT          530 a         1545 c         1609 g          571 t
ORIGIN      
        1 cgccggtgca ggcgctggcc ccggccgccc gccagagcgt gacgatcgcg cctcccggca
       61 gccgggcggc gctggtggtg gcgaaaccgg cgaccgcggc ggccccggcc gtgaaggcgc
      121 cggcactcgc gtccgctgcg aagcccgccg ccgtggcgcc agtcacggct ggcaaggccg
      181 cggcgaagcc ggcgaccgtc gccggcaagc ccccggcacc ggccgcgaag ccggtcgtcg
      241 tcgcccgggc cgccccggcc aggccggccg cctcccggac cacgccgccg gcgaaggtca
      301 cgccggtgaa ggccacgcca gcgaaggtcg cgccggtgaa ggtcgcgccg gtgaaggcca
      361 ccggcaccac ggctccggcg aaggcgacgg ccgccccggt gaagcccgcc gatgtgaaga
      421 atgcaaagga tgcaaaaaac acgaaggacg cgaagcagcc gacgacgacc gcgcagcgct
      481 gatctctgcc gtcacatccc cggaccccgc agccgcccgc gcgccgctgc ggggtccggc
      541 gtttctgacg cacgctgtag cgtgcggctg acggccggcc gtcgccgaag ctcgcgcgcc
      601 gcgcaggcag ccgctaccgt gcgcgcccgg ttcgggtccc gaccccgacc tcaatcttcc
      661 gtggtgatcc cgtgctctac gccttcgacc tgttcggtgt gttcgtcttt gcggtcagcg
      721 gcgcgctggt cgccgcgcgc aagcagctcg actggctcgg tgcgctggtg ctggccgcgg
      781 tcaccgcggt cggcggcggc acgctgcgcg acctgctgct cgcccgccat ccgctggtct
      841 ggttcagcga cgcccactac ctgctcacca tcgcgctcgc ggtcgccgcg acctgggtgt
      901 gggggcgcca gcgcccggtg ccgaagcgcg cgctgctggt ggccgatgcc gcggggctgt
      961 cgctgttcac ggtggtcggc acgcaggtcg cgctcacgca gggccagggc gcggtgatcg
     1021 cggtgctgct cggcatcacc accggtgccg ccggcggtgc gatccgcgac gtgctctgcg
     1081 ccgaggtgcc gctggtgctg cgtcaggaga tctacgcgac cgcggcggca gccggcggcc
     1141 tgctgttcgt gctgctgacc ggcctcggcc tgccggcggc cgcggccgca ctcgtcgcca
     1201 tggccgcggt gttcgcgctg cggctcgcgg cgatctaccg cgactggcat ctgccgcgct
     1261 accggctgcg ggcggaaggc gagtgagcct gccgcccgtc cgtcagcgcc ggacccacca
     1321 catgccgagg cgcagcagcg cgcggaccag ccacgggccg gcgagccggg cgatcccgaa
     1381 gccggctgcc atcggcatca ccgcctgcgc cagcacgtgc aggccggtcg cgagcagcgg
     1441 tgccgtctcc gggctgaacc cccaccaggc gcagaccgtc gcggccacgc ggctgcccat
     1501 cggggtcggg ctggcgagtg cgggcagcgt gcacatggca atgccgcgga tcgacgtgag
     1561 cgtcgtcgcc acgcttttgc cgtacgcgga caggcgctcg ccggcgatgc cgagtgccgc
     1621 gcggtgcacg gcgaaggccg ccggccagtc gggcgcggcc agcgaacgtt cgtcgatctg
     1681 caccagcacg ctgccgtcga gctgcagcag cgtggtcacg gcgacgtgat cgaggtccgg
     1741 cgtgccgtcg gcgccggtac gcgcggtcac gagctgggcg ccgtcgccgt gcagcagttc
     1801 ggcgagcagc gcgcgcaggc cggggcgcgg atagaaggcc ggggcgccgc tgcgcgaatc
     1861 gagcaggccg gccacccagc gcggcggctc agccgcggcg cttgtcgagg acattcgaga
     1921 acgcgtcgcc gagcttgccg aaggccgtgc agatcgcctc gaacagcgcg cgccagtgcg
     1981 tgagcgcggt gccgacgcca tcggcatgca gcgccttgac cgcctcgaac tgcgggtgca
     2041 gcagcagcgc cgggtcgatg ccgttcagca cgtcgccgtc gaggtggatc gtcgtctgca
     2101 tcacgacgcg ctcgccgccg aggttccaga gcttgcgcag gcgcatgcgg tcctcgggct
     2161 gcaggtcgaa gccgcggccc ggcgtcgtct gcaggtgctg ccagccgatg aaggcctgca
     2221 gccagcgcga ttcgcgtgcg atgcgccgca gcggcgcgcg tgccggcgat tcggccgggg
     2281 tctgcgcgag cagcgtcgcg gcaacatcgg cgcaactgcc gaacagcgtc gcccacagca
     2341 gcgcgccggc aaagcgctca tcgaacggtc cggccccgct ttcgaaggcc gcggcgttgc
     2401 cgggcggatg cagcgcatcg atcgcatcgg cgaggtggcc gtagggcgcg tccaggcccg
     2461 gaaaggtctg cgcgatcagc gccagcgccc ggccgatcgc ggtgtcgtgc tcggaagccg
     2521 gcggcggcgg ggccggcggt tcggccgggg cggcagcgtc ctgcagcacg gcgtcgatgt
     2581 gcacggcggc gaccgagtcg tcatcggcgt gcannnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnntcctgca gcacggcgtc gatgtgcacg gcggcgaccg agtcgtcatc
     2761 ggcgtgcacg gcgtcgatgt gcacggtgtc ggcggtcgga ttgccgggga tcggggccgg
     2821 gccggcgtcg agttgcacga gcgtggcgac gtacttctcg gcgatctcgc gcagcagcag
     2881 cggataggcc ggcggcttgc gcgcggtcat gccgggtttg aggaccgtgt tgacttcgag
     2941 cgtcatcagg tcctgcgcga tggacagcgc gatctggcgc agatcggcca tcggcgttca
     3001 gtccttgccg gtcgcgagcg cgatcagctt ctcgacggcg tcgaggttgg ccttgaccgt
     3061 cgcctgcgcg ctggcgacct gcgactggtg gaaggcgcgc agcgcatcgt cggcgccttc
     3121 gccgaagtcc gccggcacga tgttcaggat gtcgccgccg accagatcga tcgacgtgta
     3181 cagcagcttg ctgccggcgg ccggttcgta gcggccggtg gcggggtttt tcgttgcctc
     3241 gccgactgcg gtggtgatgt cgagcgtggt catgcgctcg aaggcggcgt tgagcttttc
     3301 gagcagcttg ccgcggcccg cgtcgagttc gtcggccatg atccgtacct cgcgtccctt
     3361 ccgtgcgggc cggggcggac agcggggcgc ggcgtccggg cggcggccac ccgcggcggg
     3421 cgtccgctta cgttctagtc gccgcggccg gcttggcaag cgctcacggc ggcgccgtcg
     3481 ttccacagcc aggcggcgcc gcgcacgccg ctcgaatcgc cgtgcagggc cgggcgcagc
     3541 ggcgtgtcga cggcatcggc gaacacgtgc ggcgccatca gcgccggcac gcgcgcgtag
     3601 agctcgtcga cgcgcgacag gccgccgccg agcacgatga cctccggatc gagcaggttc
     3661 accaccgttg ccagcgcccg cgccagccgc tcgcagtagc gcgccaggct ggtggcggcg
     3721 gccggatcgc cggcgcgggc gcgtccgacg atggtctcgg ccggcacgag ctgctgcagg
     3781 ccgtgctcgc gggcgtgggt gcgcgcgaag ccggggccgg acagccaggt ctcgatgcag
     3841 ccgctgcgcc cgcaccagca gagcggcccc ggatgctcgc cgatcgccgg ccacggcagc
     3901 gggttgtgtc cccactcgcc ggcgatgccg ttgtggccgt tgagcacgcg cccgtgcgcc
     3961 gccagcccgc cgccgacgcc ggtgccgagg atcaccgcga acaccagtcc ggccccggcc
     4021 gcggcaccgt cgacggcttc ggacagcgcc agacagttgg catcgttggc gaagcgcagc
     4081 ggccggttga gcagggcttc gagatcacgc gccagcggcc ggccgttcag gcaggtcgaa
     4141 ttggcgttgc gcatgcgccc gtcccgcggc gaggccgaac ccggcgtgcc gagcccgacg
     4201 ctcgcccgcc cgcccagctc ctgctcggcc gcctcgacca gcgcccggat cgcctgcagc
     4261 gtcgcggcgt aatcaccctg cggcgtcggc acgcgccggc gcaggcattc gcggccgtcg
     4321 gcatcgagcg cgagcagttc gatcttggtg ccgcc
//
LOCUS       DAOQIE010000314         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12074_length_4321_cov_6.977028, whole genome shotgun sequence.
ACCESSION   DAOQIE010000314 DAOQIE010000000
VERSION     DAOQIE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12074_length_4321_cov_6.977028"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK144_15720"
     CDS             complement(<1..434)
                     /locus_tag="PK144_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67226.1"
                     /translation="MNAVVRPPETARRHAGELELRAPWYAAASDGCTLHRYLDDDFVP
                     RFLGELAAGGPADDGWTQADRFGGSALPVLRLPVHRAFYVLATEVHCVQPGHPALDPQ
                     RIVEAGCVVRRHDPRGGLQAWMLAGGRPLGWRALALDDQAREP"
     gene            complement(439..1095)
                     /locus_tag="PK144_15725"
     CDS             complement(439..1095)
                     /locus_tag="PK144_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNF67227.1"
                     /translation="MAAEPQPHALAGAVGTLQLAIVTDTRDPENRGRVQVRLVALAVE
                     LWAPVLVASAGNGYGVACLPKIGEIVALGFVGPELPLVLGSLWTGAAPPQPGAAPVEQ
                     RYSVKTPAGTELRFDDAGSPQLLIETPAGQRLRLSDSGNGLLRIEIAGNTITVDGSSV
                     RVQAVEEISLSAPQVNISAGQLKVAAGMSQFSGVVKCDTLISNAVVSASYTPGAGNVW
                     "
     gene            complement(1085..2119)
                     /locus_tag="PK144_15730"
     CDS             complement(1085..2119)
                     /locus_tag="PK144_15730"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNF67228.1"
                     /translation="MSDSAFTAARPWCRIDGSLRADVQTAVLACTAGGRIGEPAHAEV
                     RLLNWGGRGGADAGFLFDSLQPGQTLTLGFGDDEVFTGDITAVEERYGGGAPQLVLLA
                     EDALHRLARSRHSRSFEALSPAALLTQLAAAARLQADCNVDAPVATWHQLNESDLALL
                     CRVLGRYDIEPWLDGTKLVARRPEAPTGTLALNAMDSAELRLIADLAQQPTAVEVRGY
                     DLASGSEVRGSARTLQPPPGAVTAAARLAALGWPGAEVFPQPAAGSQTDADGLAAAAF
                     GRRAGAWVHGDMRLRGNPKLRRGGYVELSGVSKRFAGRYRMTGVWQSFDLTEGFRTRL
                     KLERGDWNGS"
     gene            complement(2144..2314)
                     /locus_tag="PK144_15735"
     CDS             complement(2144..2314)
                     /locus_tag="PK144_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67229.1"
                     /translation="MPVHIEELDTTMTEPPPAPANDAQAETDARPVQPDPLLLALALE
                     LLAERRARLQFD"
     gene            complement(2386..3252)
                     /locus_tag="PK144_15740"
     CDS             complement(2386..3252)
                     /locus_tag="PK144_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67230.1"
                     /translation="MATDDPAVTRARLVPWQGNTLLDAKAISVAFNPVSLKLALSNQI
                     KADAGNKGKAQSAAQFVDKSASTLSFELIFDTSLDYSDVREQTRRIAELFMKPVAPGE
                     PGYQGDGKQPTVPRRLRFQWGKFGFDGLMQSYAETLDYFSPEGVPLRATLSLTLAEDR
                     FQHFSLPGEPPLARTPPQFAPAGGATTVPEAERAAGADPGRDWRATALFNGLETPRFT
                     AGVGVGVGAGVGVGAGVDAGVGVFVPPGPAPSLGGGFTFGASARLGTHLGAAVETPAP
                     AVRAGVSIRFGV"
     gene            complement(3287..3838)
                     /locus_tag="PK144_15745"
     CDS             complement(3287..3838)
                     /locus_tag="PK144_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNF67231.1"
                     /translation="MAEFGLSASVSVGFGGGGPGDAEASPPPAQEPVFVPFRFRVQLY
                     SADAGAPSALICAGAFSEVSGLEATMTPKAIREGGRNWGELQRAGTTSFATVVLKRGM
                     TSVGDLWNWFESVNRLSVTGYRMRATIEVLADGSDTPRLVWTLANALPVKFKGADLSA
                     SAAQVAIEELHFVHEGLTLARPG"
     gene            complement(3831..>4321)
                     /locus_tag="PK144_15750"
     CDS             complement(3831..>4321)
                     /locus_tag="PK144_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67232.1"
                     /translation="TPRSAAQLRRSPGVGVLLARDGRLVLDDERLPGGLPDTRAEQPG
                     LPQPPARTAYYRSAEVLRFVNWLLRRLDALGQRLVFVSDPDDPRPALLLEDFFARLHA
                     LGALRGARFEDACTIRRAATGAAALAFEIEIAPAVPVDAITLTLAPVDGDWRAQAVAG
                     RG"
BASE COUNT          575 a         1540 c         1622 g          584 t
ORIGIN      
        1 ggttcgcggg cctggtcatc gagcgcgagc gcccgccagc cgagcggccg gccgccggcg
       61 agcatccagg cctgcaggcc gccgcgcggg tcgtggcggc gcacgacgca gccggcctcg
      121 acgatgcgct gcgggtcgag cgccgggtgg ccgggctgca cgcagtggac ttcggtcgcg
      181 agcacgtaga acgcgcggtg caccggcagg cgcagcaccg gcagcgcact gccgccgaag
      241 cggtcggcct gcgtccagcc gtcatcggcc ggtccgccgg cggcgagttc gccgaggaaa
      301 cgcgggacga agtcgtcatc gaggtagcgg tgcagcgtgc agccatcgct cgccgctgcg
      361 taccacggcg cgcgcagttc gagttcgccg gcgtggcggc gggcggtctc gggcggacgg
      421 acgacggcgt tcatcgcttc accagacgtt gccggcgccc ggcgtgtagc tcgccgagac
      481 caccgcgttg ctgatcagcg tgtcgcactt gaccacgccg ctgaactggc tcatgccggc
      541 cgcgaccttg agctgtccgg cgctgatgtt gacctgcggg gcgctgaggc tgatctcttc
      601 cacggcctgc acgcgcacgc tgctgccatc gacggtgatc gtgttgccgg cgatctcgat
      661 gcgcagcagg ccgttgccgc tgtcgctcag gcgcaggcgc tggccggccg gggtctcgat
      721 cagcagctgc gggctgccgg cgtcatcgaa gcgcagctcg gtgccggccg gcgtcttcac
      781 gctgtagcgc tgctcgaccg gcgcggcgcc gggctggggc ggggccgcgc cggtccacag
      841 gctgccgagc accagcggca gctccgggcc gacgaagccg agcgcgacga tctcgccgat
      901 cttcggcagg caggcgacgc cgtagccgtt gccggccgag gccacgagca ccggcgccca
      961 cagctcgacc gcgagcgcaa cgaggcgcac ctgcacgcga ccacggtttt cggggtcgcg
     1021 cgtgtcggtg acgatcgcca gttgcagcgt gccgacggca ccggcgaggg cgtgcggctg
     1081 cggttcagct gccattccag tccccgcgtt cgagcttcag ccgcgtgcgg aagccttcgg
     1141 tcagatcgaa ggactgccag acgccggtca tccggtagcg cccggcgaag cgcttcgaca
     1201 cgccggacag ttccacgtag ccgccgcggc gcagcttcgg gttgccgcgc agccgcatgt
     1261 cgccgtgtac ccaggcgccg gcacggcggc cgaacgcagc ggcggcgagg ccgtcggcgt
     1321 cggtctgtga gccggcggcc ggctgcggga agacctcggc accgggccag ccgagtgcgg
     1381 ccagccgcgc ggctgcagtc accgcgccgg gcgggggctg cagcgtgcgc gccgagcccc
     1441 ggacttcgct gccgctcgca aggtcgtagc cgcgtacctc gaccgcggtt ggctgctgcg
     1501 cgaggtcggc gatcaggcgc agctcggcgc tgtccatcgc attgagtgcg agcgtgccgg
     1561 tcggcgcttc ggggcggcgg gcgacgagct tcgtgccatc gagccagggc tcgatgtcgt
     1621 agcgcccgag cacacggcag agcagcgcca ggtcgctttc gttgagctga tgccaggtcg
     1681 cgaccggcgc atcgacgttg cagtcggcct gcagccgggc ggcagcggcg agctgcgtga
     1741 gcagcgccgc cggcgacagc gcctcgaagc tgcggctgtg gcgactgcgt gccagccggt
     1801 gcagcgcgtc ttcggcgagc agcacgagtt gcggcgcgcc gccgccgtag cgttcctcga
     1861 ccgcggtgat gtcgccggtg aaaacctcgt cgtcgccgaa gccgagcgtc agcgtctggc
     1921 ccggctgcag cgaatcgaac aggaaaccgg catcggcacc gccacgcccg ccccagttca
     1981 gcagcctgac ttcggcgtgc gcgggctcgc cgatgcggcc accggccgtg caggcgagca
     2041 cggccgtctg cacgtcggcg cgcagactgc cgtcgatgcg gcaccacggg cgggcggcgg
     2101 tgaaggcgga gtcagacatc ggccgggttc ctgtaccggg gcatcagtcg aactgcagcc
     2161 gggcccggcg ctcggcgagc agttcgagcg cgagcgcgag cagcagcgga tcgggctgca
     2221 ccgggcgcgc gtcggtctcg gcctgcgcgt cgttcgccgg cgccggcggc ggctcggtca
     2281 tggtcgtgtc gagttcctcg atatgcaccg gcatcgcccg gactcctgaa agtgttcgcc
     2341 agagtgcttg ccagaaagcc cgccaaagtg ccccccggac gccgctcaga cgccgaagcg
     2401 gatcgacacg ccggcgcgca cggccggtgc cggggtttcc accgcggcgc cgagatgcgt
     2461 gccgagccgg gccgaggcgc cgaacgtgaa gccgccgccg agcgacggtg ccggacccgg
     2521 cggcacgaac acgccgaccc cggcatcgac gccggccccg acgccgactc cagccccgac
     2581 cccgacgccg actccggccg tgaaacgcgg ggtttcgagg ccgttgaaca gcgccgtcgc
     2641 gcgccagtcg cggcccggat cggcaccggc ggcgcgctcg gcctccggca ccgtcgtcgc
     2701 gccgccggcc ggggcgaact gcggcggcgt gcgcgcgagc ggcggttcgc cgggcagcga
     2761 gaagtgctgg aagcggtcct cggcgagcgt cagcgacagc gtcgcgcgca gcggcacgcc
     2821 ctcgggcgag aagtagtcga gggtttcggc gtagctctgc atcagcccgt cgaagccgaa
     2881 ctttccccac tggaagcgca gccgccgcgg tacggtcggc tgcttgccgt cgccctggta
     2941 gccgggttcg ccgggtgcga ccggcttcat gaacagctcg gcgatcctgc gcgtctgctc
     3001 gcgcacgtcg ctgtaatcga ggctggtgtc gaagatcagc tcgaagctca gcgtcgaggc
     3061 gctcttgtcg acgaactgcg cggcgctctg tgccttgccc ttgttgccgg cgtcggcctt
     3121 gatctggttg ctgagcgcga gcttcaggct caccggattg aaggcgacgc tgatcgcctt
     3181 cgcatcgagc agcgtattgc cctgccaggg cacgagccgg gcgcgggtga cggcggggtc
     3241 gtcggtggcc atcgtcgggg ttcctccgga ttcgttcggt ctcgcgtcag cccgggcgcg
     3301 ccagcgtcag cccttcgtgc acgaagtgca gttcctcgat cgcgacctgc gcggcgctcg
     3361 ccgacaggtc ggcgcccttg aacttcaccg gcagcgcgtt ggcgagcgtc cagaccaggc
     3421 gcggcgtgtc gctgccatcg gcgagcacct cgatcgtcgc gcgcatccgg tagccggtga
     3481 cgctcaggcg gttgacgctc tcgaaccagt tccacagatc gccgaccgag gtcatgccgc
     3541 gcttgagcac gacggtcgcg aagctcgtcg tgccggcgcg ctgcagctcg ccccagttgc
     3601 gcccgccctc gcggatcgcc ttcggcgtca tcgtcgcctc caggccgctg acctcgctga
     3661 acgcgccggc gcagatcagc gccgacggtg cgccggcatc ggccgaatac agctgcacgc
     3721 ggaagcggaa cgggacgaag acgggttcct gcgccggtgg cggcgaggct tcggcatcgc
     3781 cgggaccgcc gccgccgaag ccgaccgaga cgcttgccga cagtccgaac tcagccacgg
     3841 ccggccaccg cctgcgcgcg ccagtcgccg tcgaccggcg cgagcgtcag cgtgatcgca
     3901 tcgaccggca cggccggtgc gatctcgatc tcgaaggcga gggcggccgc gccggtcgcg
     3961 gcgcggcgga tcgtgcaggc gtcctcgaag cgcgctccgc gcagtgcgcc gagcgcgtgc
     4021 aggcgcgcga agaagtcttc gagcagcagc gccgggcgcg ggtcgtcggg gtcgctgacg
     4081 aacaccaggc gctgaccgag cgcatcgagc cggcgcagca gccagttcac gaagcgcagg
     4141 acttcggccg agcggtagta cgcggtgcgc gccggcggct gcggcagccc gggctgctcg
     4201 gcgcgcgtgt ccggcaggcc gccgggcagg cgctcgtcat cgagcacgag gcggccgtcg
     4261 cgcgcgagca gcacgccgac gccgggggag cggcgcagct gcgcggccga gcgcggcgtc
     4321 t
//
LOCUS       DAOQIE010000315         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12086_length_4319_cov_5.934568, whole genome shotgun sequence.
ACCESSION   DAOQIE010000315 DAOQIE010000000
VERSION     DAOQIE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12086_length_4319_cov_5.934568"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK144_15755"
     CDS             complement(<1..835)
                     /locus_tag="PK144_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF67233.1"
                     /translation="MAVTWLHVSDFHFPAKATHEQDVVQDALLTAVRRFHAQGRCPDL
                     IFVTGDIARAGQPAEYERATAYFDELLRSHHLDRNRLFVVPGNHDVDQAAGKYLLRTL
                     DNLTNANAFFDAPQSADYFRKFAAFRTWHEAYFGPSISNNSSGTGSFSTNTTCGQLVK
                     LTINDRELQILPINSALFSQDKTDKGKLWIIPKCLRDVFAALDESPDTVRIALLHHPF
                     DWLHEAERTQIEGLLQQRFDIVLRGHMHETDVRQVITGSGTVLHLAAGAVYQGSERPK
                     KA"
     gene            complement(951..1328)
                     /locus_tag="PK144_15760"
     CDS             complement(951..1328)
                     /locus_tag="PK144_15760"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNF67234.1"
                     /translation="MIRAARWLTLACWAGLFLFWLARPIWLVPTEAPAIPLKLLLAGA
                     LLFPLRGLLHGKPYTHGWACFLALGYFVLSVDDLAGGLASKPLGWTGLLLAVGFFFGC
                     MGYARLEGRRRKVMAGFAAAPKG"
     gene            complement(1325..1930)
                     /gene="wrbA"
                     /locus_tag="PK144_15765"
     CDS             complement(1325..1930)
                     /gene="wrbA"
                     /locus_tag="PK144_15765"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNF67235.1"
                     /translation="MTAPAAEILVLYYSRHGATAEMARLVARGVEEVPGMQARLRTVP
                     AVSAVCEKTADDIPAEGPPYATLADLRECAGLALGSPTRFGNMAAPLKFFLDGTSELW
                     ISGALAGKPAGVFTSTGSLHGGQETTLLTMMLPLLHHGMLIVGLPYTETVLHTTRTGG
                     TPYGPSHFAGTEARPSPDEKTLCRALGRRLAQTAQRLAVPS"
     gene            complement(1927..2307)
                     /gene="arsC"
                     /locus_tag="PK144_15770"
     CDS             complement(1927..2307)
                     /gene="arsC"
                     /locus_tag="PK144_15770"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746396.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNF67236.1"
                     /translation="MSATETPSSKLSFTIYHNPRCSKSRAALDLLSQHGIEPAVVRYL
                     DTPPDAATLAQLLDQLGLEPRALLRSGEDEYKTLNLADPALSREQLIAAMVAHPKLIE
                     RPIVVHDERAVLGRPPERVLELLR"
     gene            2645..3160
                     /locus_tag="PK144_15775"
     CDS             2645..3160
                     /locus_tag="PK144_15775"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF67237.1"
                     /translation="MRRSVLAATLLFTSALAGAEPVDLTLPTTDGSTLSLSSLRGRWV
                     VVNVWATWCSPCIKELPELAAFHAEHKSKDATVVGVAYENTTAGMLSKFIEPYKLGYP
                     VMMAGTTPLPGFPKMKGVPTTFILNPKGELVRTYTGPVTAAGVELLIDDLREPAKPAA
                     AIHDTPARPAT"
     gene            3168..3455
                     /locus_tag="PK144_15780"
     CDS             3168..3455
                     /locus_tag="PK144_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNF67238.1"
                     /translation="MHRSAAAVICRRFHVSGRVQGVSFRAYTQARALELGLSGYARNL
                     PDGRVEVLACGSEPAVGQLHDWLWTGSPAAQVESVTVSEALFEAHAGFDRG"
BASE COUNT          721 a         1403 c         1454 g          741 t
ORIGIN      
        1 tagccttctt cgggcgttcg ctaccttgat acaccgcgcc ggcggcgaga tgcagaacag
       61 tgcctgagcc ggtaatgact tgcctgacat cggtttcatg catgtgtccg cgcaggacga
      121 tatcgaagcg ttgttgcagc aatccctcga tctgtgtgcg ctctgcttcg tgcagccagt
      181 caaatggatg atgaagcagg gctatccgta cggtatcagg cgactcgtcg agggcggcaa
      241 agacgtcacg caggcatttt gggatgatcc acagtttgcc cttgtcggtc ttgtcctgtg
      301 agaacaatgc gctgttgatc ggcagaatct gcaactcacg atcattgatc gtgagtttca
      361 ccagttgccc gcaggtggtg ttcgtcgaaa aactgcctgt tcctgatgag ttattgctga
      421 tggatggccc gaaataagcc tcgtgccaag tccggaacgc ggcgaacttc ctgaaataat
      481 cggcggactg cggagcgtcg aagaacgcat tggcgttggt cagattgtct agcgtacgca
      541 gcaaatactt gccggcagct tggtcaacgt cgtggttgcc ggggaccaca aacagacgat
      601 tacgatccag atgatggctg cgcagcagtt cgtcaaaata ggcggtggcg cgctcgtatt
      661 cggctggctg accggctctg gcgatatcgc ctgttacaaa gatcagatcg gggcagcgtc
      721 cctgcgcgtg gaagcgtctg acggcggtaa gcaaggcatc ctgaacgacg tcctgctcat
      781 gggttgcttt ggccgggaaa tgaaagtccg acacatgcag ccacgtaacc gccatcttcc
      841 gcgcctccgc gtggtccatt tccttcgcgg agccggatcg accgtgtgcc cgcgcgctcg
      901 tcgggccagc gtaggcgagg gccgtgccgt gcgctacgtg ctgcgctgca tcagcctttc
      961 ggcgccgcgg cgaacccggc catcaccttc cgccgccttc cctccagccg cgcatacccc
     1021 atgcacccaa agaaaaaccc caccgccagc agcagcccgg tccagccgag cggcttgctg
     1081 gcaagcccgc cggcgagatc atcgaccgac agcacgaaat agccgagcgc gaggaagcac
     1141 gcccagccgt gcgtgtacgg cttgccgtgc agcaggccgc gcagcggaaa cagcagggcg
     1201 ccggcgagca ggagcttgag cgggatcgcc ggggcctcgg tcggcaccag ccagatcggg
     1261 cgggcgagcc agaacaggaa caggcccgcc cagcaggcga gcgtcagcca gcgggcagcg
     1321 cggatcatga cggcaccgcc agtcgctgcg cggtctgcgc cagccgccgg ccgagggcgc
     1381 ggcagagggt tttctcgtcg gggctcgggc gggcttcggt gccggcgaag tggctggggc
     1441 cgtagggcgt gccgccggtg cgcgtcgtgt gcagcaccgt ttcggtgtag ggcaggccga
     1501 cgatcagcat gccgtggtgc agcagcggca gcatcatcgt cagcagcgtg gtctcctggc
     1561 cgccgtgcag gctgccggtg gacgtgaaca cgccggccgg cttgccggcg agcgcgccgg
     1621 agatccacag ctccgaagtg ccatcgagga agaacttcag cggtgccgcc atgttgccga
     1681 agcgcgtcgg gctgccgagc gcaagccccg cgcattcgcg caggtcggcg agcgtggcat
     1741 acggcggacc ttcggccggg atgtcgtcgg cggtcttctc gcagaccgcc gagaccgccg
     1801 gcaccgtgcg cagccgcgcc tgcatgccgg ggacttcctc gacgccgcgc gccaccagcc
     1861 gcgccatctc ggccgtggca ccgtggcgcg agtaatagag caccagaatc tcggccgccg
     1921 gcgccgtcat cgcagcaact ccagcacgcg ctcgggcggg cggccgagca cggcccgctc
     1981 gtcatgcacg acgatcggcc gctcgatcag cttcggatgc gcgaccatcg ccgcaatcag
     2041 ctgctcgcgg ctcagggccg gatcagccag gttcagcgtc ttgtattcgt cctcgccgct
     2101 gcgcagcagc gcgcgcggct ccagcccgag ttgatcgagc aactgcgcga gcgtggcggc
     2161 atccggcggc gtgtccagat agcgcacgac ggccggctcg atgccgtgct gactcagcag
     2221 gtcgagggcg gcgcgcgact tcgagcagcg cgggttgtgg tagatcgtga acgagagctt
     2281 ggacgagggc gtttcggttg cggacatgcg gggaaggacc ggattctgcg aggtcggagc
     2341 cgggagccgg ctgaggctgc cggatcaggg aggaggctgg cgtcgccgtg acctcggccg
     2401 gcggcgccgc gcggcatctt agcggcatcc gccgcagccg tgatccgcaa ccgggcgtcg
     2461 aacctttgca gcgcccggcc cgtcagtggc tgaagcaggc gccggctcgt ctccggggtg
     2521 gcgcctgtgg tcgggcccac cgccggtgcc cgacgcgcac tggcacgctg atcgtgcgcg
     2581 ccgccgccgg acgcaaggct gcgctaggat tacaaaacct tcattcgttt gaggagaagc
     2641 tgtcgtgcgc cgatccgtcc tcgccgccac gctgctgttc acgtcggccc tcgccggtgc
     2701 cgaacccgta gacctgaccc tgccgaccac cgatggctcg acgctcagcc tgtcctcgct
     2761 gcgcggccgc tgggtggtcg tcaacgtctg ggcgacctgg tgcagcccct gcatcaagga
     2821 actgccggaa ctggccgcct tccacgccga gcacaagagc aaggatgcaa cggtggtcgg
     2881 cgtcgcctat gagaacacca cggccggcat gctttcgaag ttcatcgagc cgtacaagct
     2941 cggctatccg gtcatgatgg ccggcaccac gccgctgccg ggcttcccga agatgaaggg
     3001 tgtgccgacg accttcatcc tcaatccgaa gggcgagctg gtgcgcacct acacgggtcc
     3061 ggtcaccgcc gccggcgtcg aactgctgat cgatgacctg cgcgagccgg ccaagccggc
     3121 cgctgccatc cacgacaccc cggcccggcc ggccacctga gtcccccatg caccgatccg
     3181 ctgccgccgt gatctgccgc cgtttccatg tctccgggcg cgtgcagggc gtgtcgttcc
     3241 gcgcgtacac gcaggcacgt gccctcgaac tcggcctgag cggctacgcc cgcaacctgc
     3301 cggatggccg ggtcgaggtg ctggcctgcg gcagcgaacc ggccgtcggc cagctgcacg
     3361 actggctgtg gaccggctcg ccggctgcgc aggtcgaatc ggtgacggtc agcgaagcgc
     3421 tgttcgaggc acacgcaggc ttcgatcgcg gctgaatgcc ggtctcgtgg tcgagccggc
     3481 cgcagcatcg ggacttgact tatgcacaaa gcccggtgca ggcgctgcat ccggcgcagg
     3541 cgcacgccgc cttgtggaat gatccggcaa ggccttgatt taattgcatt gatcaactac
     3601 gcggcagcgc cgtcgatcac gccaaaaagc ctgagcgggc atgggcttgg cattttgtcc
     3661 ccgaagtttt ccacaggctt gtccacggct tctgtggaca agcaggaaag tgctggcagg
     3721 gcaggggctt gcggcgcggg gatgatctgc actgcatgct gccgtggtgc cggaggtggg
     3781 actcgaaccc acatgccttg cggcggtcga ttttgagtcg agtccgcaac cataaaataa
     3841 cttttatatc aacggcttgc gttgataaaa cccgaattga cgcacgactg cgacacgcat
     3901 tctgcaacgc agcgcaatgg gtagcaacat gcgtcgagcc gtcgatttgc cagtgttttg
     3961 ccggcctgaa ctattcatat ggctacgcct cgggcggttg ccgtctgcgt gcatctatct
     4021 gttaagccgc acaaatcagg ttgcgcttca tccctcgcgc cgggtcagtt cgccctgttg
     4081 gcggtcggcc ggcgcgcgtc ccgtacttcg acgatgacgg caggcgcgtc gggtgcgagg
     4141 ccggccagag cgcgggcttc ggcctccgac tgcccacact gaacgataag caccggcagc
     4201 gggtgcgccg gggtgcggtc ggtgcgctcg cgctccagag cttgcaggcg ggcatgcagg
     4261 gtgcgcgccg ccgtcacggg ccgtcacgcg tcagttcagc ctgctggcgg atggcctcc
//
LOCUS       DAOQIE010000316         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12301_length_4272_cov_8.744605, whole genome shotgun sequence.
ACCESSION   DAOQIE010000316 DAOQIE010000000
VERSION     DAOQIE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12301_length_4272_cov_8.744605"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1451)
                     /gene="pmbA"
                     /locus_tag="PK144_15785"
     CDS             complement(51..1451)
                     /gene="pmbA"
                     /locus_tag="PK144_15785"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNF67239.1"
                     /translation="MPDSQLSPASASLPATAPSALPERAELDRLVGMILDEARRLGAT
                     ESEAAVSFSDGLSVSVRLGEVETLEYQRDRGLGVTVYFGRRKGSASTSDWREPALLET
                     VRAACDIARYTAEDPWSGLADPARLARAPLPELDLHHPWALSAEQAIDIARDCEAAAR
                     GFDARISNSDGASVGRHESLVVYGNSNGFLGGYPSTRHSLSCSVIAADPQADGRMERD
                     YWYSSDRDASRLEAPDAVGRRAAERVLRRLGARRIGTRTAPVLFVPELARGLVGHFLG
                     AIRGGALYRKSSFLLDRAGTAVFPDFMQIHERPHLPRALGSAAFDGEGVATQDRDLVC
                     DGVLQGYVLDSYSARRLGLETTGNAGGVHNVIVGSSAPAVGYDELLAAMGTGLVVTEL
                     MGHGVNAVTGDYSRGAAGFWVENGEPVHPVEEITIAGNLKTMFETLQMIGSDIDLRGN
                     VRCGSMLLAPMTIAGA"
     gene            1712..2236
                     /gene="yjgA"
                     /locus_tag="PK144_15790"
     CDS             1712..2236
                     /gene="yjgA"
                     /locus_tag="PK144_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNF67240.1"
                     /translation="MNRSDDFADTGDTQPEGPSKSARKREAEALQTLGERLVGLPEAQ
                     LRQVPLPEDLAGAIAECRRISAHGGRYRQMQYIGKLMRRIDPQPILDVLARFDSTSAE
                     SKRRQHQVERWVEAMVAGDETVLGEYLTAYPQADRQQIRQLIRNAQRELSTGKPPKSR
                     RELFRLLRAGAWQD"
     gene            complement(2319..3926)
                     /locus_tag="PK144_15795"
     CDS             complement(2319..3926)
                     /locus_tag="PK144_15795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNF67241.1"
                     /translation="MPAPASGPVRRRPAAAGGFRSLPLAGALLCGLGLAVPAQAFSIL
                     GVNAGGNLTSALGVDEMFRWNGIATVGGVRTIGYRLDPGFTLAQQAAIDRAFATWNTV
                     ASAQPNMIASPGAFDLETVAVHEIGHALGLAHSGVREGDTPNPRFFDTDQNAFNLMPN
                     PDRTTLARNVTSRPTVDFFPGPGLSSSVMSYDASLAPGYLRTQLEHDDVGGLRYAGAG
                     PDNLSGNGDDFEYRFVRINAAPIQDFNAFGQLVPGIDIVRNPTEHIDAGIIDVFNGNP
                     FGAGGIADMVYDRTGFTGNGTFSILGGDIYLSATTLEDPYRSFPGVFTFTDPACDADD
                     VCNGLGTAHFDTGQNGRTPFNVNFDYDSAVYPVQPAGVFRLGTLAVTNGETFAGSVPE
                     IGNTSNFHLNLHLASSDGANVGDLLLGYYSSPNVFPNLRDPGNADILYDARHPEFGGV
                     YILEGNTLAEALTNVNLLGHLGSIEFDGFELPPGPFADGNSFLAPLPGGSFTGPQGSF
                     LIGVPEPASLLLLSAALPLLHRRRCPA"
BASE COUNT          645 a         1459 c         1572 g          596 t
ORIGIN      
        1 cggcatgccg cgccgcgcgc gtggcatgcc gctacaggca ccgcggtcgc tcacgccccc
       61 gcgatcgtca tcggcgcgag cagcatcgag ccgcagcgca cgttgccgcg caggtcgatg
      121 tcgctgccga tcatctgcag cgtctcgaac atcgtcttca ggttgccggc gatggtgatt
      181 tcctcgaccg ggtgcaccgg ctcgccgttc tcgacccaga agccggccgc cccgcgcgag
      241 tagtcgccgg tgacggcgtt gacgccgtgg cccatcagtt cggtgacgac caggccggtg
      301 cccatcgccg cgagcagttc gtcgtatccg acggccggcg cactcgaccc gacgatgacg
      361 ttgtgcacgc caccggcgtt gccggtggtt tcgaggccga gccggcgggc cgaatacgaa
      421 tcgagcacgt agccctgcag cacgccgtcg cagaccagat cgcgatcctg cgtggcgacg
      481 ccctcgccgt cgaaggcggc gctgccgagg gcgcgcggca ggtgcgggcg ctcgtggatc
      541 tgcatgaagt ccggaaacac cgcggtgccg gcgcgatcga gcaggaagct cgacttgcgg
      601 tacagcgcgc cgccgcggat cgcgccgagg aagtgcccga ccaggccgcg cgccagctcc
      661 ggcacgaaca gcaccggcgc cgtgcgcgtg ccgatgcgcc gggcgccgag gcggcgcagc
      721 acgcgctcgg ccgcgcgccg gccgaccgca tccggggctt cgaggcggct ggcgtcgcgg
      781 tcgctgctgt accagtaatc gcgctccatg cggccgtcgg cctgcgggtc ggcggcgatc
      841 accgagcacg acagcgaatg gcgtgtcgac ggatagccgc cgaggaagcc gttcgagttg
      901 ccgtagacga ccagcgactc gtgccggccg accgaggcgc catcgctgtt gctgatgcgc
      961 gcatcgaagc cgcgtgccgc cgcctcgcag tcgcgggcga tgtcgatggc ctgctcggcg
     1021 ctgagcgccc acgggtgatg caggtcgagt tccggcagcg gcgcgcgcgc cagccgggcc
     1081 ggatcggcga ggccggacca cgggtcctcg gccgtgtagc gggcgatgtc gcaggcggcg
     1141 cgcacggttt cgagcagcgc cggctcgcgc cagtccgagg tgctcgccga gcccttgcgc
     1201 ctgccgaaat agacggtgac gccgaggccg cggtcgcgct ggtattcgag ggtctcgacc
     1261 tcgccgaggc gcaccgacac cgacaggccg tcgctgaagc tgacggcggc ctcggactcg
     1321 gtggcaccga gccggcgcgc ctcgtcgagg atcatcccga ccagccggtc gagttcggcg
     1381 cgctcgggca gggccgacgg cgccgtggcg ggcagggacg cactggctgg gctgagctgg
     1441 gaatccggca tggcgatcgt tcctggacat cggaagggca gggccgccac ccggcacggg
     1501 cagcggcgtg gcacccgtgc ggctgccggt cgtggaccgc acgggcgtga tgttgctggc
     1561 aaggcacggg gccgacacgg cgcctgcgac gcgctaccat cgcgcccccg tttttctgcc
     1621 tccctgcccg ggcggccctg accggcctgg tccggatacc cacccgcagg cgccggacac
     1681 tgcccggcac cggtgtggag atcgctcccc gatgaaccgt tccgacgatt tcgccgacac
     1741 cggcgacacg cagcccgaag gcccgagcaa gtccgcgcgc aagcgcgagg ccgaagccct
     1801 gcagacgctc ggcgagcggc tggtcggcct gcccgaggcg cagctgcggc aggtgccgct
     1861 gcccgaggat ctcgccggcg cgatcgccga atgccggcgc atcagcgcac acggcggacg
     1921 ctaccgtcag atgcagtaca tcggcaagct gatgcgacgc atcgatccgc agccgatcct
     1981 cgacgtgctc gcgcgcttcg actcgaccag cgccgagtcg aagcgccgcc agcatcaggt
     2041 cgagcgctgg gtcgaggcga tggtcgccgg cgacgagacc gtgctcgggg agtacctgac
     2101 cgcttacccg caggccgatc gccagcagat ccgccagctg atccgcaatg cccagcgcga
     2161 actctcgacc ggcaagccgc cgaagtcgcg ccgcgagctg ttccggctgc tgcgcgccgg
     2221 cgcctggcag gactgatccg gcaagccggc cggtcagccc gcgcggcggc ccccggcagg
     2281 caggggccgg gggccggtcg tgcggacaga ggcaggcttc aggccgggca gcggcgccga
     2341 tgcagcagcg gcagcgccgc cgacagcagc agcagcgacg ccggctccgg cacgccgatc
     2401 aggaagctgc cctgcggtcc cgtgaacgaa ccgcccggca gcggcgcgag gaagctgttg
     2461 ccgtcggcga acggcccggg cggcagttcg aagccgtcga actcgatcga gccgagatgc
     2521 ccgagcaggt tgacgttggt cagcgcctcg gcaagcgtgt tgccttccag gatgtacacg
     2581 ccgccgaact ccggatggcg cgcgtcgtag aggatgtcgg cgttgcccgg atcgcgcagg
     2641 ttcgggaaca cgttcggcga cgagtaatag ccgagcagca ggtcgccgac gttggcgccg
     2701 tcgctcgacg ccagatgcag gttcaggtgg aagttgctgg tgttgccgat ctccggcacc
     2761 gagcctgcga aggtttcgcc gttggtcacg gccagcgtgc cgagccggaa cacgccggcc
     2821 ggctgcaccg gatagacggc gctgtcgtag tcgaagttga cgttgaacgg cgtgcggccg
     2881 ttctggccgg tatcgaaatg cgcggtgccg agaccgttgc agacgtcgtc ggcgtcgcag
     2941 gccggatcgg tgaacgtgaa cacgcccggg aagctccggt acgggtcctc cagcgtcgtc
     3001 gcgctcaggt agatgtcgcc gccgaggatc gagaacgtgc cgttgccggt gaagccggtg
     3061 cggtcgtaga ccatgtcggc gatgccgccg gcaccgaagg ggttgccgtt gaacacgtcg
     3121 atgatgccgg catcgatgtg ttcggtcggg ttgcggacga tgtcgatgcc gggcacgagc
     3181 tggccgaagg cgttgaaatc ctggatcggc gcagcattga tgcgcacgaa gcggtattcg
     3241 aaatcatcgc cgttgccgga gaggttgtcg gggccggcac cggcatagcg caggccgccg
     3301 acatcgtcgt gttcgagctg cgtgcgcaga tagcccggcg cgaggctggc gtcgtagctc
     3361 atcacgctcg aggacaggcc gggaccgggg aagaaatcca cggtcgggcg gctcgtgacg
     3421 ttgcgcgcca gcgtcgtgcg atccggattc ggcatcaggt tgaaggcgtt ctggtcggtg
     3481 tcgaagaagc gcggattcgg cgtgtcgccc tcgcgcacgc cggagtgcgc gagcccgagc
     3541 gcatggccga tctcgtgcac tgcgacggtt tcgagatcga aggcccccgg cgacgcgatc
     3601 atgttcggct gcgcgctcgc gaccgtgttc caggtcgcga acgcccggtc gatcgccgcc
     3661 tgctgcgcca gcgtgaaacc cggatcgagc cggtagccga tggtcctgac gccaccgacc
     3721 gtcgcgatgc cgttccagcg gaacatctcg tcgacgccga gcgcgctggt gaggttgccg
     3781 ccggcgttga cgccgaggat cgagaaggcc tgcgccggca ccgcgaggcc gagcccgcag
     3841 agcagcgcgc cggcgagcgg gaggctgcgg aaaccgccgg cggcagccgg tcgccggcgg
     3901 acggggccgg acgcgggagc cggcacatga ttcgcggcag gaacagggaa gggatcagac
     3961 gaaagaagca gtgcggtgcg cttcatgacg gcccccgtcg gtgagcgacc gcggcgtgaa
     4021 ccggtctcct cccccgctcg gtccgggtgc ctccgccctg tgtctttgcc tgcctggccc
     4081 tgaccgccgg gcgacgccgg tgcgccgccg tacggacccc ggacgcaggg cccgtgcccg
     4141 gtggacacgg gtcgcatgtc cgccgcaccg gcgagacgcc ggttgcaacg gagattgcat
     4201 caggcaggcc acgtccggca tttgcctttc aatcatacgt ttgcgcagag acaccccgac
     4261 tgtaccgggc tg
//
LOCUS       DAOQIE010000317         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12387_length_4251_cov_7.137512, whole genome shotgun sequence.
ACCESSION   DAOQIE010000317 DAOQIE010000000
VERSION     DAOQIE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12387_length_4251_cov_7.137512"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PK144_15800"
     CDS             complement(<1..895)
                     /locus_tag="PK144_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNF67242.1"
                     /translation="MRTAALALLLGTLAVHALPLLPARNWCIALPLVAALALWPRLPW
                     LLRLPAVALSGLLWTLWRLPDPGLALPPAYDGQTFVLEGRIAALPEHDHRRLELELET
                     TEARAADATLVPLAGRVRLAWYEAPAEDLPQVGERWRFTARLRAPYGFANPGGFDRER
                     HLYASGVVARGSVRSQPPPARLAGAEAHLIDRLRARFAAALQRLRPADPGTAILAALT
                     IGEEGAISTHQWETFRRTGTTHLMAISGQQIALVAGLMFVAVRALWARLPGLAARLAP
                     VPAGALAALLSALGYALIAGMG"
     gene            complement(924..1640)
                     /gene="lolD"
                     /locus_tag="PK144_15805"
     CDS             complement(924..1640)
                     /gene="lolD"
                     /locus_tag="PK144_15805"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNF67243.1"
                     /translation="MNDAALLDPALATGTPVLRAVGLCKTFRQGDTEVEVFTGVNLEV
                     PRGQRIAIVGASGSGKSTLLHLLGGLDRPTRGEVWIGDHDIARLSDAARGRLRNRRLG
                     FVYQFHHLLPEFTALENVAIPRVIGGEKPALAQAAARRLLERVGLGHRLAHKPGALSG
                     GERQRAAIARALVTDPECVLADEPTGNLDRHNAEAVFELMLELNRERSTSFVIVTHDE
                     HLAARLDATWRLDAGTLSRG"
     gene            complement(1633..2880)
                     /locus_tag="PK144_15810"
     CDS             complement(1633..2880)
                     /locus_tag="PK144_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNF67244.1"
                     /translation="MFQPLEVFIGLRYVRAKRSNHFISFISASSMLGIALGITALITV
                     LSVMNGFQQEVRERILAMTAHATISGVAGRLDDWREVRKAANANAEVVASAPYIRAES
                     MLSAGSATAGTIVQGVLPDEEQSVSDIATKITAGRWDDLQPGRFGIVLGRWLAQRLGV
                     GIGDKVTVLVPQANVTPAGVLPRLKRFDVVAIFESGMYEYDSGLALIHIDDAAKLLQM
                     EGSVSGLRLKLKDLFRAPLIVREIGSELPGAYWVRDWTQEHANFFRAVQIEKTAMFVI
                     LMLIVAVAAFNLVSTLVMVVTDKQADIAILRTLGVTPAGIMTIFVVQGTIIGFIGTAL
                     GVAGGVALALNVREAVAWLEGLLHIKFLSPEVYPITELPSQLQWGDVWTIAPVAFVLA
                     LLATLYPAWRAARTQPAEALRYE"
     gene            complement(2983..3348)
                     /locus_tag="PK144_15815"
     CDS             complement(2983..3348)
                     /locus_tag="PK144_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF67245.1"
                     /translation="MKLFHHLLKPLLGCLLGLWFTISVAAPLDINTATAAELATVMQN
                     VGPKKAEAIVAWREQNGPFKSVDELEKVPGIGPKIVEANRDRLTVDTPAAAATPAPAP
                     AMPAPAAAPAAPAAPVKKP"
     gene            complement(3497..4225)
                     /gene="pyrF"
                     /locus_tag="PK144_15820"
     CDS             complement(3497..4225)
                     /gene="pyrF"
                     /locus_tag="PK144_15820"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNF67246.1"
                     /translation="MTTPSPQARDPRLIVALDYPQAAPALALAARLDPALCRLKVGKE
                     LFTAAGPALVEALQQRGHEVFLDLKFHDIPNTVAGAVRAAAGLGVWMVNVHASGGRRM
                     LEAARAAVAAPARPPLLIAVTVLTSLERADLAETGLDIEPAAQVDRLAALSRDCGLDG
                     VVCSAQEAARLRALCGPAFRLVTPGIRPASSEAGDQRRTMTPADALAAGADYLVIGRP
                     VTGAPDPAAALADIAAGLANIGRT"
BASE COUNT          631 a         1450 c         1592 g          578 t
ORIGIN      
        1 ggcccatgcc ggcgatcagc gcatagccga gcgcgctcag cagcgccgcc agcgcaccgg
       61 ccggcaccgg cgccagccgc gccgcgagcc ccggcagccg tgcccagagc gcacgcaccg
      121 cgacgaacat caggccggcg accagcgcga tctgctggcc ggagatcgcc atcaggtgcg
      181 tggtgccggt gcgacggaag gtttcccact gatgcgtgct gatcgcgcct tcctcgccga
      241 tcgtcagtgc agcgaggatc gcggtgccgg ggtcggccgg gcgcaggcgc tgcagcgcgg
      301 cagcgaagcg ggcgcgcagg cggtcgatga gatgcgcctc ggcaccggcc agccgtgcgg
      361 gcgggggctg gctgcgcacg ctgccgcgcg cgacgacgcc gctggcgtac agatgccgct
      421 cgcggtcgaa accgccggga ttggcgaagc cgtagggcgc acgcagccgc gccgtgaagc
      481 gccagcgctc gccgacctgc ggcaggtctt cggccggggc ctcgtaccat gcaagccgca
      541 cgcgtccggc cagcggcacg agcgtggcgt cggcggcgcg ggcctcggtg gtttcgagtt
      601 cgagttcgag ccgccggtga tcgtgttcgg gcagcgcagc gatgcggcct tcgagaacga
      661 aggtctgtcc gtcgtacgcc gggggcagtg ccagccccgg gtcgggcagg cgccacagcg
      721 tccacagcag accggacaag gccactgccg gcaggcgcag cagccacggc aggcgtggcc
      781 acagcgccag cgccgcgacc aggggcagcg cgatgcacca gttgcgtgcc ggcagcagcg
      841 gcagcgcgtg gactgccagc gtgcccagga gcagcgcgag ggcggccgtg cgcatcgggc
      901 ggcatccgtg ccggtgcgcc gcttcagccg cggctcagcg tgccggcatc gagccgccag
      961 gttgcatcga gccgcgcggc gaggtgctcg tcgtgcgtga cgatgacgaa cgaggtcgag
     1021 cgttcgcggt tcagctccag catcagctcg aacacggcct cggcgttgtg gcggtcgagg
     1081 ttgccggtcg gctcgtcggc gagcacgcat tcggggtcgg tgaccagcgc ccgcgcgatc
     1141 gccgcacgct ggcgctcgcc gccggagagc gcgccgggct tgtgcgcgag ccggtgaccg
     1201 agaccgacgc gttcgagcag ccggcgcgcg gcggcctgcg cgagcgccgg cttctcgccg
     1261 ccgatcacgc gcgggatggc gacgttctcg agggcggtga attccggcag caggtgatgg
     1321 aactggtaga cgaagccgag ccggcggttg cgcagccgcc cgcgcgcggc gtccgacagc
     1381 cgggcgatgt cgtgatcgcc gatccagacc tcgccgcgcg tcggccggtc gagaccgccg
     1441 agcaggtgca gcagcgtgct cttgcccgag ccgctggcgc cgacgatcgc gatgcgctgg
     1501 ccgcgcggca cttcgaggtt cacaccggtg aacacctcga cctcggtgtc gccctggcgg
     1561 aaggtcttgc agaggccgac ggcgcgcagc accggcgtgc cggtcgcgag tgcagggtcg
     1621 agcagggcgg catcattcat agcgcagggc ctccgccggc tgcgtgcgcg cggcgcgcca
     1681 ggccggatac agcgtcgcga gcagcgccag cacgaaggcc accggcgcga tggtccagac
     1741 atcgccccac tgcagctgcg agggcagctc ggtgatcgga tagacctcgg gcgacaggaa
     1801 cttgatgtgc agcagacctt cgagccaggc gaccgcctcg cgcacgttca gtgccagcgc
     1861 cacgccgccg gcgacgccga gcgcggtgcc gatgaagccg atgatcgtgc cctgcacgac
     1921 gaagatggtc atgatcccgg ccggcgtgac gccgagcgtg cgcaggatcg cgatgtcggc
     1981 ctgcttgtcg gtgacgacca tcaccagcgt cgagaccaga ttgaaggccg cgaccgcgac
     2041 gatcagcatc aggatcacga acatcgcggt cttctcgatc tgcacggcgc ggaagaagtt
     2101 ggcgtgctcc tgcgtccagt cgcgcaccca gtaggcgccg ggcagctcgc tgccgatctc
     2161 gcgcacgatc agcggcgcgc ggaacaggtc cttcagcttc aggcgcaggc ccgagaccga
     2221 gccttccatc tgcagcagct tcgcggcgtc atcgatgtgg atcagcgcca gtccgctgtc
     2281 gtactcgtac atgccggatt cgaagatcgc gaccacgtcg aagcgcttca gccgcggcag
     2341 cacgccggcc ggcgtgacgt tggcctgcgg cactagcacc gtgaccttgt cgccgatgcc
     2401 gacgccgagg cgctgcgcga gccagcgccc gagcacgatg ccgaagcgcc cgggctgcag
     2461 atcgtcccag cgtccggcgg tgatcttggt ggcgatgtcg gagacgctct gctcctcgtc
     2521 gggcagcacg ccctggacga tggtcccggc cgtggcgctg ccggcgctca gcatcgattc
     2581 ggcgcggatg tacggggcgc tggcgacgac ctcggcgttg gcgttggcgg ccttgcgcac
     2641 ctcgcgccag tcatcgagcc ggccggcgac accggagatc gtcgcgtgcg cggtcatcgc
     2701 caggatgcgt tcgcgcacct cctgctgaaa gccgttcatg accgacagca ccgtgatcag
     2761 cgccgtgatg ccgagcgcga tgccgagcat cgacgacgcg gagatgaacg agatgaagtg
     2821 attggagcgt ttggcccgca cgtaacgcag gccgatgaac acttccagcg gctggaacat
     2881 cctgagcatg cgaggggata ccgggtgacg gggacggccc gccgcccggc gagagggcgg
     2941 cggggccggc ggagtatacc ggccggcccc gcggcagacg gcttaaggct tcttgaccgg
     3001 agctgccggt gcagccggag cggccgcagg tgcaggcatc gccggggccg gcgccggggt
     3061 ggcagcggcg gccggcgtgt cgacggtcag ccggtcgcgg ttggcctcga cgatcttcgg
     3121 tccgatgccc gggaccttct ccagctcatc gaccgacttg aacgggccgt tctgttcgcg
     3181 ccaggcgacg atggcctcgg ccttcttcgg accgacgttc tgcatgacgg tcgcgagttc
     3241 cgcggcggtg gcggtgttga tgtcgagcgg ggctgcaacg gaaatggtga accacaggcc
     3301 gagcaggcag cccagcagcg gcttgagcag gtgatggaac agcttcatga cggattcctc
     3361 ccgatggatg tccggatggc cgggaggcgg ggccggtgcg atccggaccc tgaggcttcc
     3421 ctgccatgtc agccggaggg ctgacggggt tcaggctaga tcgccgtcct gaaacttcgg
     3481 ttgcatggat gaaacttcat gtcctgccga tatttgcgag cccggcagcg atgtcggcga
     3541 gcgcagcggc cgggtcgggc gcgccggtga ccggccggcc gatgaccaga taatcggcac
     3601 cggccgcgag cgcgtcggcc ggggtcatcg tgcgccgctg gtcgccggcc tcgctgctcg
     3661 ccggccggat gccgggcgtc accagccgga aggccgggcc gcacagcgcc cgcagccggg
     3721 cggcttcctg cgccgagcag accacgccat cgaggccgca gtcgcgcgac aacgcagcca
     3781 gccggtcgac ctgcgcggcc ggctcgatgt cgagtccggt ttcggcgaga tcggcacgct
     3841 ccagactggt cagcaccgtc accgcgatca gcagcggcgg acgcgccggg gcagcgacgg
     3901 ccgcgcgcgc ggcttcgagc atgcgccgtc cgcccgaggc atggacgttg accatccaga
     3961 ccccgagccc ggcggccgcg cgcacggctc cggcgacggt gttcgggatg tcgtggaact
     4021 tgaggtcgag gaacacctca tggccgcgct gttgcagggc ctcgaccagc gccggtccgg
     4081 cggccgtgaa cagttccttg ccgaccttga gccggcacag cgccgggtcc agccgtgccg
     4141 ccagcgccag cgccggcgcg gcctgcgggt aatcgagcgc gacgatcagg cgcgggtcgc
     4201 gcgcctgcgg ggacggggtg gtcatcggtg cagaatctcc ggcgggggac g
//
LOCUS       DAOQIE010000318         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12497_length_4227_cov_7.002397, whole genome shotgun sequence.
ACCESSION   DAOQIE010000318 DAOQIE010000000
VERSION     DAOQIE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12497_length_4227_cov_7.002397"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1125
                     /gene="dprA"
                     /locus_tag="PK144_15825"
     CDS             37..1125
                     /gene="dprA"
                     /locus_tag="PK144_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNF67247.1"
                     /translation="MLAPNVILAVTATRTFSGVVVEDSYLASQDFDRLKAAQIALASP
                     DAAAIERELTWAGRPGNRLLLPGSPAYPPLLALAPRPPVLLWVIGEPALLADPQLAVV
                     GARRPSAGGRDIARSFAAQLAASGLVITSGLALGIDAAAHRGALEAGGRTLAVLGTGL
                     DRIYPAANRDLAHAIVDGRGALVSEFPLGTPALADNFPRRNRVIAGLSLGTLVVEAAL
                     ASGSLITARLAAEHNREVFAVPGSIHNPLARGCHALIREGAKLVESAQDILAELAPRL
                     QALLSAPPAAPAQVSGTALPAGVAAPAALDEDYRKLLDLLADSPAPVDLLVRRSGLTA
                     EIVSSMLLILELEGYVATAPGGLYSRLV"
     gene            1137..1613
                     /locus_tag="PK144_15830"
     CDS             1137..1613
                     /locus_tag="PK144_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNF67248.1"
                     /translation="MKENVLDVLMYLFQNFIEEDLDTDTDRATMHSELVDAGFASREV
                     HAAFDWLDSLVERRNTLSAPVHPERSFRIYTEQECARLDTACRGLLLQLEQAGVLGPE
                     NRELVIDRVLALPAAEIDEAQLKWLILMVLFRQPGQEDACAAIEDLLFDDFGAWLH"
     gene            1715..4069
                     /locus_tag="PK144_15835"
     CDS             1715..4069
                     /locus_tag="PK144_15835"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HNF67249.1"
                     /translation="MSKHLVIVESPAKAKTIKKYLGKDYEVLASYGHVRDLVPKEGAV
                     DPERGFAMKYEVIDKNQKHVDAIAKALAKADALYLATDPDREGEAISWHLYEILRERE
                     LLADKPVQRVVFHEITKRAIQEAITHPRELAMDLVNAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLIVEREEEIERFVTREYWTIEADCAKQQPFGAKLVEYQGAK
                     LTQFSVENAPSAHAMREALLAEAQGRLQVAKVEKKQRKRNPAAPFTTSTLQQEASRKL
                     GFSASRTMRTAQALYEGIDIGSGSVGLITYMRTDSVNLAAEALTEIRELIARRYGAHN
                     VPPEPRVYKTKAKNAQEAHEAIRPTSVAIEPEQVRAHLSAEQFKLYELIWKRTVACQM
                     IPATLDTVSVDLACGPGKGNTFRASGSTIADPGFLAVYEEGVDDARTREADDEEGGRV
                     LPPLAVGDVVELRAIRAEQHFTEPPPRYSEASLVKALEEFGIGRPSTYAAILSTLQAR
                     EYAVIDGRRFVPTDVGRIVNRFLTQHFTQYVDYDFTARLEDELDEVSRGEKEWVPLMQ
                     EFWGPFRERCEDKEGSVSRTEVVQARELGSDPKTGKPVSVRIGRFGPFVQIGTKDDAD
                     KPQFASLRPEQRMDSITLEDALELFKLPRDLGKTADGEPIKANVGRFGPYVQYGKKYV
                     SLKEDDPYSVTRERALELIAAHKEAEANKYIRVFDGSPVQVLNGRYGPYITDGKKNAK
                     IPKDREPAALTLPECLELLAAAPDKKPGARGAARGGARGKAKAG"
     gene            4069..>4227
                     /locus_tag="PK144_15840"
     CDS             4069..>4227
                     /locus_tag="PK144_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNF67250.1"
                     /translation="MAQIALARAARVLAAGGIVAYPTEAVYGLGCDPLDFDAVARLLA
                     LKRRPVEKG"
BASE COUNT          685 a         1494 c         1460 g          588 t
ORIGIN      
        1 agcgtcaaag ccaagccagc gatgcccggc aaggtcatgc tggcgccgaa cgtgatcctg
       61 gcggtaacgg cgacgcggac cttctcgggg gtggtggtcg aggacagcta cctcgcgagc
      121 caggacttcg accggctgaa ggccgcgcag atcgccctcg ccagtcccga tgccgctgcc
      181 atcgagcgcg agctgacctg ggcaggacgg ccgggaaacc ggctgctgct tcccggctcg
      241 ccggcctatc cgccgctgct cgccctcgcg ccgcgcccgc cggtgctgct gtgggtgatc
      301 ggtgaacccg cgctgctcgc cgatccgcag ctcgccgtcg tcggtgcgcg ccgcccgagc
      361 gccggcgggc gcgacatcgc ccgcagcttc gccgcgcagc tcgccgccag cggcctcgtc
      421 atcaccagcg ggctggcgct cggcatcgac gctgccgcgc accgcggcgc gctcgaagcc
      481 ggcggccgca cgctggccgt gctcggcacc gggctcgacc gcatctatcc ggccgccaac
      541 cgcgatctcg cgcatgcgat cgtcgacggc cgcggcgcgc tggtctcgga gttcccgctc
      601 ggcacgccgg cactcgccga caactttccg cgccgcaacc gcgtgatcgc cgggctcagc
      661 ctcggcacgc tggtggtcga ggcggcgctc gccagcggtt cgctgatcac ggcgcggctc
      721 gccgccgagc acaaccgcga ggtgttcgcg gtgccgggct cgatccacaa cccgctggcg
      781 cgcggctgcc acgcgctgat ccgcgagggg gcgaagctcg tcgagtcggc gcaggacatc
      841 ctcgccgagc tggcgccgcg gctgcaggcg ctgctgtcgg cgccgccggc cgcaccggcg
      901 caggtgtccg gcacagcgct gccggcaggg gtggcggccc cggccgcgct cgacgaggac
      961 taccgcaagc tgctggattt gctggcggat tcgcctgcac cggtcgacct gctggtgcgg
     1021 cgcagcggat tgacggccga aatcgtttcc tccatgctgc tgattcttga gctggaaggg
     1081 tacgtcgcca cggcaccggg cgggctgtat tcacggctcg tctgattgag gtggtgatga
     1141 aagaaaacgt cctcgacgtg ctgatgtacc tgttccagaa cttcatcgaa gaggacctcg
     1201 acaccgacac cgaccgcgcg accatgcaca gcgagctggt cgatgccggc tttgcgtcgc
     1261 gtgaggtgca cgctgcgttc gactggctcg acagtctcgt cgagcggcgc aacacgctga
     1321 gtgcgccggt gcatccggaa cggtccttcc gcatctacac cgaacaggaa tgcgcacggc
     1381 tcgacaccgc ctgccgtggc ctgctgctgc agctcgaaca ggccggcgtg ctcggcccgg
     1441 agaaccgcga gctggtcatc gaccgcgtgc tggcgctgcc ggcggccgag atcgacgagg
     1501 cgcagctcaa gtggctgatc ctgatggtgc tgttccgcca gcccggtcag gaggacgcct
     1561 gcgccgcgat cgaggatctg ctgttcgatg atttcggagc ctggctccac taggctcagc
     1621 agccgcgcgt gaccgccccg gtgcgcgcgc cggcgccgcg tagcacccac ccggcccgac
     1681 cctcccggcc ctccccgagc gcggtaccgc gaccatgagc aagcatctgg tcatcgtcga
     1741 atcgccggcc aaggcgaaga cgatcaagaa atatctcggc aaggactacg aggtgctcgc
     1801 ctcctacggc catgtccgcg atctggtgcc gaaggaaggc gccgtcgatc ccgagcgcgg
     1861 cttcgcgatg aagtacgagg tcatcgacaa gaaccagaag cacgtcgatg cgatcgcgaa
     1921 ggcgctggcc aaggccgatg cgctgtatct ggcgaccgac ccggaccgcg agggcgaggc
     1981 gatctcgtgg catctgtacg agatcctgcg cgagcgcgag ctgctcgccg acaagccggt
     2041 gcagcgcgtg gtgttccacg agatcaccaa gcgcgcgatc caggaggcga tcacccatcc
     2101 gcgtgagctg gcgatggatc tggtcaacgc ccagcaggcg cggcgcgcgc tcgattatct
     2161 ggtcggcttc aacctgtcgc cgctgctgtg gaagaagatc cgccgcggcc tgtcggccgg
     2221 gcgcgtgcag tccccggcgc tgcgcctgat cgtcgagcgc gaggaagaga tcgagcgctt
     2281 cgtcacgcgc gagtactgga cgatcgaggc cgactgcgcg aagcagcagc ccttcggcgc
     2341 caagctggtc gaataccagg gcgcgaagct cacgcagttc agcgtcgaga acgccccgtc
     2401 cgcccatgcc atgcgcgagg ccctgctcgc cgaggcgcag ggcaggctgc aggtcgcgaa
     2461 ggtcgagaag aagcagcgca agcgcaatcc ggccgcgccg ttcacgacct cgacgctgca
     2521 gcaggaggcc tcgcgcaagc tcggcttttc ggccagccgc acgatgcgca ccgcgcaggc
     2581 gctgtacgag ggcatcgaca tcggctcggg cagcgtcggc ctgatcacct acatgcgcac
     2641 cgactcggtc aacctcgccg ccgaggcgct gaccgagatc cgcgagctga tcgcacgcag
     2701 gtacggggcg cacaacgtgc cgcccgagcc gcgcgtctac aagaccaagg ccaagaacgc
     2761 ccaggaagcg cacgaggcga tccgcccgac ctcggtcgcg atcgagcccg agcaggtgcg
     2821 cgcgcacctc tccgccgagc agttcaagct ctacgagctg atctggaagc gcacggtcgc
     2881 ctgccagatg atcccggcga cgctcgacac ggtctcggtc gatctcgcct gcgggcccgg
     2941 caagggcaac accttccgcg ccagcggctc gaccatcgcc gacccaggct tcctcgccgt
     3001 ctacgaggaa ggcgtcgacg atgcccgcac gcgcgaggcc gacgacgagg agggcgggcg
     3061 cgtgctgccg ccgctcgcgg tcggcgacgt ggtcgagctg cgcgcgatcc gcgccgagca
     3121 gcacttcacc gagccgccgc cgcgctacag cgaggcgagc ctggtcaagg cgctggagga
     3181 gttcggcatc ggccggccct cgacctacgc cgcgatcctc tcgacgctgc aggcgcgcga
     3241 atacgcggtc atcgacggcc gccgcttcgt gccgaccgat gtcgggcgca tcgtcaaccg
     3301 cttcctgacc cagcacttca cgcagtacgt cgattacgac ttcacggccc ggctggagga
     3361 cgaactcgac gaggtctcgc gcggcgaaaa ggaatgggtg ccgctgatgc aggagttctg
     3421 ggggccgttc cgcgagcgct gcgaggacaa ggaaggctcg gtgtcgcgca ccgaggtcgt
     3481 gcaggcgcgc gaactcggca gcgacccgaa gaccggcaag ccggtgtcgg tgcgcatcgg
     3541 ccgcttcggg cccttcgtgc agatcggcac caaggacgac gccgacaagc cgcagttcgc
     3601 gagcctgcgc cccgagcagc gcatggattc gatcacgctc gaggacgcgc tcgaactgtt
     3661 caagctgccg cgcgacctcg gcaagaccgc cgacggcgag ccgatcaagg ccaacgtcgg
     3721 gcgcttcggg ccgtacgtgc agtacggcaa gaagtacgtg tcgctgaagg aggacgatcc
     3781 gtactcggtc acgcgcgagc gcgcgctgga actgatcgcc gcgcacaagg aagccgaggc
     3841 caacaagtac atccgcgtgt tcgacggctc gccggtgcag gtgctgaacg gccgctacgg
     3901 cccgtacatc accgatggca agaagaacgc caagatcccc aaggaccgcg agccggcggc
     3961 gctgacgctg cccgagtgcc tcgaactgct ggccgccgcc cccgacaaga agcccggtgc
     4021 gcgcggtgcg gcccgcggtg gtgcccgcgg caaggccaag gccggctgat ggcgcaaatc
     4081 gcgctcgcgc gcgccgcgcg cgtgctcgcg gccggcggca tcgttgccta cccgaccgag
     4141 gccgtgtacg ggctcggctg cgacccgctc gatttcgacg cggtcgcgcg cctgctggcg
     4201 ctgaagcgcc ggccggtcga gaagggc
//
LOCUS       DAOQIE010000319         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12609_length_4206_cov_7.466635, whole genome shotgun sequence.
ACCESSION   DAOQIE010000319 DAOQIE010000000
VERSION     DAOQIE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12609_length_4206_cov_7.466635"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PK144_15845"
     CDS             complement(<1..1246)
                     /locus_tag="PK144_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNF67251.1"
                     /translation="MNACIPPAIPGAAAAVLPASAVLSAPLAPRRWHARPGTRLIALV
                     AVVVSLGCALLLGLDQHRLRNELLDQVSDGYVRTSGLLAAQVSGGVRFGRPQAIEQAY
                     ASLSGAAEPVLDALVVFDASGRPLAEYRAPRATGAALSAWPALESGKARPAQPLVRQV
                     ADGLEVALAVQSGEATVGLLAVRWSLAEVDAVLRTALWQQLGVAIALLTVLVGGLVLA
                     IRRLMVAPLARINTAVEQLAAGRLDTQVPETARHDELGDMARAVGVLRENALARVRLE
                     AEQRELMLEQQRLREAEQRAESARQEARIAAQAAERDAEARRQHETEARERAAAAERQ
                     AAMHALAERFESSVQRVVDTVSKTAQQVHATAAELVSNAAATGRDAGSVAQASQETAA
                     QVQTMSSACEELATSVREIGGRA"
     gene            complement(1269..1877)
                     /locus_tag="PK144_15850"
     CDS             complement(1269..1877)
                     /locus_tag="PK144_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001926473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67252.1"
                     /translation="MPPKYKTHRRIHGLAAVIAGLLLCSAAGAEGSHEVAARKFVEGT
                     LRAWMADAVVVETLKAQNAGHAALTQADIDRLDQAWRAETKAADKPMINGLLGNPLSK
                     YLAGRARASKGLVTELFVMDERGLNAGQSDLTSDYWQGDEAKWQKTYALGPGAVFIDK
                     VDLDESTQRFQVQVSATVVDPASGAAIGAVTVGLDAEALEMQ"
     gene            complement(2180..2671)
                     /locus_tag="PK144_15855"
     CDS             complement(2180..2671)
                     /locus_tag="PK144_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67253.1"
                     /translation="MTETERNGWYTLNPHCDLQIEHGVPVRIACEPGNVTANRPALAE
                     DVQRYTGLHVELGPWQAGERGTTREAALQVAAEDFDDVLARYAHASAATYWDRYQQPV
                     HARTLDDFETEAYALDFVTAMHHCGLDWRDVDKHAHSAGWQRALYSEAQRLAAYAELP
                     AQP"
     gene            complement(2732..3958)
                     /locus_tag="PK144_15860"
     CDS             complement(2732..3958)
                     /locus_tag="PK144_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNF67254.1"
                     /translation="MLNAIWIGFLLVALAGGFWEATFGGRPAVLGEMTRASFEMAKTG
                     FEISLGLTGVMTLWLGLLRIGEHAGLVSALTRLLAPLFARLMPEVPRGHPAQGAVTMN
                     MAANVLGLDNAATPLGLQAMRELQSLNPQPQRASDAQILFLVINASSVTLFPLAVFTY
                     RAQLGAADPTDVFLPILIATGCSTLAGLIACACWQRLALWQPVVLAYLGGIGLLLAAL
                     VAWVAGEPAGMAERSALLANGALLALIGAFVAAAAWRRLDAFSVFIEGAQEGFTTAVK
                     IIPYLVAMLVAIGLLRASGVLGLVLDALRALVGGFGFDARWVDAVPVMLLKPLSGSGA
                     RGMMIETMQAHGVDSFAGRLACIVQGSTETTFYVLAVYFGSVGVRITRHALACALIAD
                     AAGFFAAVTVAYAFFG"
     gene            complement(4013..>4206)
                     /locus_tag="PK144_15865"
     CDS             complement(4013..>4206)
                     /locus_tag="PK144_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNF67255.1"
                     /translation="TTPTAAAGASGAAAPAGLAAAGRDGQDSGAAGPSPSLIERALAA
                     YADAARALAGIGAGFHLVA"
BASE COUNT          616 a         1507 c         1523 g          560 t
ORIGIN      
        1 gggcgcgacc gccgatctcg cgcaccgagg ttgcgagttc ctcgcaggcg ctcgacatgg
       61 tctgcacctg cgcggcggtt tcctgcgaag cctgcgcgac gctgccggca tcgcggccgg
      121 tggcggcagc gttgctgacc agctcggcgg cggtcgcgtg cacctgctgc gcggttttcg
      181 acaccgtgtc gacgacgcgc tgcacgctgc tttcgaagcg ttcggccagc gcgtgcatcg
      241 ccgcctgacg ctcggcggcg gcggcgcgct cgcgggcttc ggtctcgtgc tggcggcggg
      301 cctcggcgtc gcgctcggca gcctgggcgg cgatccgggc ctcctggcgc gcgctctcgg
      361 cgcgctgctc ggcctcgcgc aggcgctgct gttcgagcat cagctcgcgc tgttcggctt
      421 cgaggcgcac gcgcgccagc gcgttctcgc gcagcacgcc gacggcgcgc gccatgtcgc
      481 cgagttcgtc gtggcgggcg gtctccggca cctgcgtatc cagccggccg gcggcgagct
      541 gttccaccgc cgtgttgatg cgcgccagcg gcgcgaccat cagccggcgg atcgccagca
      601 ccaggccgcc gacgagcacc gtcagcaggg cgatggccac gccgagctgc tgccagagcg
      661 ccgtgcgcag cacggcgtcg acctcggcga ggctccagcg caccgccagc aggccgaccg
      721 tcgcctcccc ggactgcacg gcgagcgcga cctccagacc atcggccacc tgccggacca
      781 gaggctgcgc cggccgggcc ttgccggact ccagggccgg ccaggccgaa agcgcggcgc
      841 cggtggcgcg cggcgcgcgg tattcggcga gcggcctgcc gctggcatcg aagaccacca
      901 gcgcgtcgag caccggctcg gcggcgccgc tcaggctcgc ataggcctgc tcgatcgcct
      961 gcgggcggcc gaagcgcacg ccgccggaaa cctgcgcggc cagcagcccg ctggtgcgca
     1021 cgtagccatc gctgacctga tcgagcagtt cgttgcgcag gcgatgctga tcgaggccga
     1081 gcagcagcgc gcagccgagg ctgacgacga cggcgaccag cgcgatcagc cgcgtgccgg
     1141 gacgcgcatg ccagcgccgc ggcgcaagag gcgcggacag gacggcgctg gcgggcagca
     1201 cggccgcggc cgcaccgggg atggcggggg gaatgcaggc gttcatgacc ggatcgatct
     1261 ccgcggactc actgcatttc cagggcttcg gcatcgaggc cgacggtcac ggcaccgatg
     1321 gctgccccgc tcgccggatc gacgacggtg gcgctgacct gcacctggaa gcgctgcgtg
     1381 gactcatcga gatcgacctt gtcgatgaac accgcgcccg ggccgagcgc gtaggtcttc
     1441 tgccacttgg cctcgtcgcc ctgccagtaa tccgaggtca ggtcgctctg gccggcgttg
     1501 aggccacgct catccatcac gaacagctcg gtgaccagcc ccttgctggc ccgggcgcgt
     1561 ccggcgaggt acttcgacag cgggttgccg agcaggccgt tgatcatcgg cttgtcggcg
     1621 gctttggtct cggcacgcca ggcctggtcg agccggtcga tgtcggcctg cgtcagcgcc
     1681 gcgtgtccgg cgttctgcgc cttcagcgtc tcgacgacga ccgcatcggc catccacgcg
     1741 cgcagcgtgc cttcgacgaa cttgcgggcg gcaacctcat ggctgccttc ggcgccggcc
     1801 gcggagcaca gcagcaggcc ggcgatgacg gcagcaaggc catgaatacg gcgatgggtc
     1861 ttgtacttcg ggggcatggc gttctccttc ctcccggatc cctccggaga ctttcgttat
     1921 gggcaggcag atcaccggcc gcagcagggc ggacgatcag aaccatcatc ggcagcccgg
     1981 cgcgactgtg aagggcgagc ggccgcgagt gctcccggca cgcgcagggc gggaccggct
     2041 gcggaagcgc aaccggccgg cagcagaaag gatttacgga accgtcactt gagccgtgga
     2101 ttcgccgctg ccggaaagcg ggatcccggc ggcgaccgta gccgccaccg cgatcacagt
     2161 caggtttttt tacacaagat cacggttgcg cgggcagttc cgcgtacgcg gcgaggcgtt
     2221 gcgcctcgct gtacagcgcc cgctgccagc cggcgctgtg ggcgtgcttg tcgacatcgc
     2281 gccagtcgag accgcagtgg tgcatcgcgg tgacgaaatc gagcgcgtag gcctcggtct
     2341 cgaagtcgtc gagcgtgcgg gcgtgcaccg gctgctggta gcgatcccag taggtcgcgg
     2401 ccgaggcatg cgcgtagcgc gcgagcacgt catcgaagtc ctcggcggcg acctgcagcg
     2461 ccgcctcgcg cgtcgtgccg cgctcgccgg cctgccaggg gccgagttcg acgtgcagcc
     2521 cggtgtagcg ctgcacgtcc tcggccaggg ccggccggtt cgccgtgacg ttgcccggct
     2581 cgcaggcgat gcgcaccggc acgccgtgct cgatctgcag gtcgcagtgc gggttcaggg
     2641 tgtaccagcc gttgcgttcg gtttcggtca tggtggccac tccttcagtg ggcggcggac
     2701 tccagggtcc gccgccgaca agtcaagcac ctcagccgaa aaacgcgtag gccacggtca
     2761 ccgcagcgaa gaatccggcg gcatcggcga tcagcgcgca ggcgagcgcg tggcgcgtga
     2821 tgcgcacgcc gaccgagccg aagtagaccg cgagcacgta gaaggtcgtc tcggtcgagc
     2881 cctgcacgat gcaggcgagc cgcccggcga agctgtcgac gccgtgcgcc tgcatggtct
     2941 cgatcatcat gccgcgcgcg ccgctgccgg acaggggctt gagcagcatc accggcaccg
     3001 catcgaccca gcgcgcatcg aagccgaagc cgccgaccag cgcgcgcagg gcatcgagca
     3061 cgaggccgag cacgccgctc gcgcgcagca ggccgatcgc caccagcatc gcgaccaggt
     3121 acgggatgat cttcacggcg gtcgtgaagc cctcctgcgc gccctcgatg aacaccgaga
     3181 acgcatcgag ccgccgccac gcggccgccg cgacgaaggc gccgatcagc gcgagcagcg
     3241 cgccgttggc gagcagcgcc gagcgctccg ccatgccggc cggctcgccg gcgacccagg
     3301 ccacgagcgc ggcgagcagc aggccgatgc cgccgagata cgcgagcacc accggctgcc
     3361 acagcgccag acgctgccag caggcgcagg cgatcagccc ggccagcgtc gaacagccgg
     3421 tcgcgatcag gatcggcagg aacacgtcgg tcggatcggc ggccccgagc tgggcgcggt
     3481 acgtgaacac cgccagcgga aacagcgtga cgctggaggc gttgatgacg aggaacagga
     3541 tctgcgcatc gctcgcgcgc tgcggctgcg gattcagcga ctgcagctcg cgcatcgcct
     3601 gcaggccgag cggggtggcg gcgttgtcga ggccgagcac gttggccgcc atgttcatcg
     3661 tcaccgcgcc ctgcgccgga tgcccgcgcg gcacctcggg catcagccgc gcaaacagcg
     3721 gcgccagcag ccgcgtcagc gccgagacca gcccggcgtg ctcgccgatg cgcagcagcc
     3781 cgagccacag cgtcatgacg ccggtcagcc cgaggctgat ctcgaagccg gtcttcgcca
     3841 tctcgaagct ggcccgggtc atctcgccga gcacggcggg ccggccgccg aacgtcgcct
     3901 cccagaaacc gcccgccagc gcgaccagca ggaagccgat ccagattgca ttcagcatgc
     3961 cgccctcccg cggaccttgc gcggggcgcg agcgtacggg attgcgggcg gctcaggcga
     4021 cgagatggaa gccggcaccg atgccggcga gcgcgcgggc ggcgtcggca taggccgcga
     4081 gcgcgcgctc gatcagcgac ggcgaaggtc cggccgcgcc gctgtcctgc ccgtcgcggc
     4141 cggcggcagc cagccctgcc ggggcagcgg ctccggatgc gccggcggct gcggtcgggg
     4201 tcgtgc
//
LOCUS       DAOQIE010000320         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12646_length_4201_cov_8.357453, whole genome shotgun sequence.
ACCESSION   DAOQIE010000320 DAOQIE010000000
VERSION     DAOQIE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12646_length_4201_cov_8.357453"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..652
                     /locus_tag="PK144_15870"
     CDS             149..652
                     /locus_tag="PK144_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF67256.1"
                     /translation="MKLTDKLLPFAALAVVLGLVAFWLLPGGVNAAPAATFRTLDGRT
                     LTTADLKGKPAIVTFWATSCPGCMEEMPHLIELYRELQPKGLQIVGVAMSYDPPSNVM
                     ELVRQKQVPYPIALDTDEAIARAFGTIRVTPTSFLIAPDGRIVQQKIGAFDLPALKTQ
                     LKSMLGA"
     gene            780..1217
                     /locus_tag="PK144_15875"
     CDS             780..1217
                     /locus_tag="PK144_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNF67257.1"
                     /translation="MSYLAIKAWHLIWMVCWFAGLFYLPRLYVYHAMLPPEDGAGHER
                     FVLMERKLFWGITTPAAVLTIGFGLWLLAAGGWALYSKMGWLHLKLALVALLVVYHVW
                     CYRYLRAFRELTNTRTHRWFRWFNEAPVIVLIAVVLLASLKPF"
     gene            complement(1222..2043)
                     /locus_tag="PK144_15880"
     CDS             complement(1222..2043)
                     /locus_tag="PK144_15880"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNF67258.1"
                     /translation="MSVPVAPGPVPGRPGWRDPRLLQMVFQATLLAVGVGLRDFSLAG
                     AQAALAFAGGLGAQLFWLQVLGLRGVGLRSALITCFGIVLLLRADGLWQHPLAAALAI
                     SAKFVLRVRGRHLWNPANFGVVLALVLFPGAWASPGQWGQDLALALWFVALGGLVSLR
                     ARSSDLAWTFLLAFLGLVALRVAWLGQSWAIWWHQLGNGALLLFAFFMISDPMTAPDH
                     PRARRLYACLVALVAALWQYGLFRPSGPLWALFLCAPLVPLFDRIWPGPRYRWGD"
     gene            complement(2040..3398)
                     /locus_tag="PK144_15885"
     CDS             complement(2040..3398)
                     /locus_tag="PK144_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNF67259.1"
                     /translation="MIRPIAAALAGAALLACTQGAAAFCGFYVGKADASLFNEASQVI
                     VVRDGPRTVISMLNDYRGEPTDFALVVPVPQVLQKGQVHVGDKKIFERLDAYSAPRLV
                     EYFDPDPCRPPVVYAPAPTMAVQEGSAVRRRGADALGVTVESSFTVGEYDIVILSAKQ
                     SDGLETWLHDNGYRIPAGAAKALAPYIKQDMKFFVAKVNLKEQAKTGFTYLRPLQFAF
                     ESEKFMLPLRLGMINASGPQDLVAFMLTRKGRVETSNYRTVKLPANVDVPEFVKGEFP
                     AFYKALFGEQATREGLKAVFTEYFWDMAWCDPCAADPLSPAELRQAGVFWVGEGAQPP
                     AVNPRIAPMPPGGGAQPVMLTRLHLRYTPDTFPEDLMFQETADRQNFQARYVLRHAAE
                     VAPDACPQAKTYLEQLGQRREQEAQQLASLTGWNINDIRRKAKLDGPAPTAPKWWENL
                     WK"
     gene            complement(3466..4098)
                     /locus_tag="PK144_15890"
     CDS             complement(3466..4098)
                     /locus_tag="PK144_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67260.1"
                     /translation="MSRFPRRVSARFVVLLSAGLLPLSALHAQAGAGSADLPPDPLDA
                     RIEEARNAYGAGDYDTSIAALETVLAELRLRKSNAYERWLPAPPKGWQASRPEAGAAP
                     QPAAGGIRAERRYTHGDEQLQLTITGESALLQGLGAVFDNPAALGSGPRIERLDGRRA
                     LYDPAGNALRVLVGNDAILVEARGNAAVSEATLQAFLRRVDFDTIEKSLR"
BASE COUNT          619 a         1531 c         1452 g          599 t
ORIGIN      
        1 tcgtctggct gctgccgcgg ctctggaggg gtatccggcg cgtgctatcc tcgctggccc
       61 gctggttcgg cggggggcgc acgccgccgg ccgatccgcc gtcactccct ccgcggctgc
      121 cgccgcctgc tccctgatca cccgcgccat gaaactcacc gacaagctgc tgccgttcgc
      181 cgccctcgcc gtcgtcctcg gcctcgtggc gttctggctg ctgccgggcg gcgtcaacgc
      241 cgccccggcc gcgaccttcc gcacgctcga cggccgcacg ctgaccacgg ccgacctcaa
      301 gggcaagccg gcgatcgtga ccttctgggc cacgagctgc cccggctgca tggaggaaat
      361 gccgcacctg atcgagctgt accgcgagct gcagccgaag ggcctgcaga tcgtcggcgt
      421 cgcgatgagc tacgacccgc cgagcaacgt catggaactg gtgcgccaga agcaggtgcc
      481 ctatccgatc gcgctcgaca ccgacgaggc catcgcccgc gccttcggca cgatccgcgt
      541 gacgccgacc tcgttcctga tcgcgccgga cgggcgcatc gtgcagcaga agatcggtgc
      601 cttcgacctg ccggcgctga agacgcagct gaagtcgatg ctcggcgcct gaaccccgcg
      661 gccctgcggc cggtccatcc gctgcgacac gggcacccgg tggtgcccgt tttgctttgt
      721 cctgccgcca cggcacccca cgctttcccg ccccgccttc ctgcacggag acccgaccga
      781 tgtcctatct cgcgatcaag gcctggcacc tgatctggat ggtctgctgg ttcgccggcc
      841 tgttctacct gccgcggctg tacgtctatc acgcgatgct gccgcccgag gacggcgccg
      901 gccacgagcg cttcgtgctc atggagcgca agctgttctg gggcatcacg acaccggcag
      961 cggtgctgac gatcggcttc ggcctgtggc tgctcgcggc cggcggctgg gcgctgtatt
     1021 cgaagatggg ctggctgcac ctgaagctcg cgctggtcgc gctgctcgtc gtctaccacg
     1081 tctggtgcta ccgctacctg cgcgccttcc gcgaactgac caacacgcgc acgcaccggt
     1141 ggttccgctg gttcaacgag gcaccggtca tcgtgctgat cgcggtcgtg ctgctcgcga
     1201 gcctgaagcc gttctgagcg ctcagtcgcc ccagcgatag cgcggccccg gccagatccg
     1261 gtcgaacagc ggcaccagcg gcgcgcacag gaacagcgcc cacagcgggc cgctcggccg
     1321 gaacagcccg tactgccaga gcgccgccac cagcgcgacc agacaggcgt acagccggcg
     1381 cgcgcgcgga tgatccggcg ccgtcatcgg gtccgagatc atgaagaacg cgaacagcag
     1441 cagggcgccg ttgccgagct gatgccacca gatcgcccag gactggccga gccacgcgac
     1501 gcgcagcgcc acgaggccga ggaaggcgag caggaaggtc caggccagat cgctgctgcg
     1561 cgcgcgcagg ctgaccagcc cgccgagcgc gacgaaccac agcgccagcg ccagatcctg
     1621 cccccactgc cccggcgagg cccaggcgcc cgggaacagc acgagcgcga gcacgacgcc
     1681 gaagttggcc gggttccaga ggtgtctgcc gcgcacgcgc agcacgaact tggcgctgat
     1741 ggccagcgcc gcggccagcg ggtgctgcca gaggccgtcg gcgcgcagca gcagcacgat
     1801 gccgaagcag gtgatcagcg ccgagcgcag gccgacgccg cgcagcccga gcacctgcag
     1861 ccagaagagc tgcgcgccga gtccgccggc gaaggcgagc gcggcctgcg ccccggccag
     1921 cgagaaatcg cgcaggccga cgccgaccgc gagcagcgtc gcctggaaaa ccatctgcag
     1981 cagccgcgga tcgcgccagc cgggccggcc gggcaccggc cccggcgcga ccggcacgct
     2041 cacttccaca ggttctccca ccacttcggg gccgtcgggg ccggcccatc gagcttcgcc
     2101 ttgcggcgga tgtcattgat gttccagccg gtcaggctcg cgagctgctg cgcttcctgc
     2161 tcgcggcgct ggccgagctg ttcgaggtag gtcttcgcct gcggacaggc atcgggcgcg
     2221 acctcggcgg cgtggcgcag cacgtagcgc gcctggaagt tctggcggtc ggcggtttcc
     2281 tggaacatca ggtcttccgg gaaggtgtcc ggcgtgtagc gcaggtgcag gcgcgtcagc
     2341 atcaccggct gcgcgccgcc gcccggcggc atcggcgcga tgcgcggatt caccgccggc
     2401 ggctgggcgc cctcgccgac ccagaacacg ccggcctggc gcagctcggc cggcgacagc
     2461 ggatcggccg cgcagggatc gcaccaggcc atgtcccaga agtattcggt gaacaccgcc
     2521 ttcaggccct cgcgcgtcgc ctgctcgccg aacagcgcct tgtagaaggc cgggaactcg
     2581 cccttcacga actccggcac atcgacgttg gccggcagct tcacggtgcg gtagttgctg
     2641 gtctcgacgc ggcccttgcg cgtcagcatg aacgcgacca gatcctgcgg gccgctggcg
     2701 ttgatcatgc cgaggcgcag cggcagcatg aatttttccg attcgaacgc gaactgcagc
     2761 ggccgcagat acgtgaagcc ggtcttcgcc tgctccttca ggttcacctt ggcaacgaag
     2821 aacttcatgt cctgcttgat atacggcgcc agcgccttcg cggccccggc cggaatccgg
     2881 tagccgttgt cgtgcagcca ggtttcgagg ccgtcggact gcttcgccga caggatgacg
     2941 atgtcgtact cgccgaccgt gaagctcgat tcgaccgtga cgccgagcgc atcggccccg
     3001 cgccggcgga ccgccgagcc ctcctgcacg gccatggtcg gggccggcgc gtagaccacc
     3061 ggcggccggc agggatcggg atcgaagtac tcgaccagcc tcggcgccga ataggcgtcg
     3121 agccgctcga agatcttctt gtcgccgacg tgcacctgcc ccttctgcag cacctgcggc
     3181 accggcacga ccagcgcgaa gtcggtcggc tcgcccctgt agtcgttcag catcgagatc
     3241 acggtgcgcg gaccgtcgcg cacgacgatg acctgcgagg cctcgttgaa caggctggcg
     3301 tcggccttgc cgacgtagaa gccgcagaag gccgcggccc cctgcgtgca cgcgagcagc
     3361 gccgcaccgg cgagtgcggc ggcgatcgga cggatcatgg gcgggactcc gtgaaaatcc
     3421 ggggcggatg gcgcagcagc gtagagcccg cgcacgcgcc cggcctcagc gcagggattt
     3481 ttcgatcgtg tcgaagtcga cgcggcgcag gaaggcctgc agcgtcgcct cgctgacggc
     3541 ggcattgccg cgcgcctcga cgaggatcgc gtcattgccg accagcacgc gcagcgcgtt
     3601 gccggccggg tcgtacagcg cgcggcggcc atcgagcctt tcgatgcgcg gaccgctgcc
     3661 gagcgcggcc gggttgtcga acaccgcgcc gagcccctgc agcagcgccg actcgccggt
     3721 gatcgtcagc tgcagctgct cgtcgccgtg cgtgtagcgg cgctcggcgc ggatgccacc
     3781 ggcggccggc tgcggcgcgg ccccggcctc ggggcggctc gcctgccagc ccttcggcgg
     3841 cgccggcagc cagcgctcgt aggcgttgct cttgcgcagg cgcagctcgg cgagcacggt
     3901 ttcgagcgcc gcgatcgagg tgtcgtagtc cccggcgccg taggcgttgc gcgcctcctc
     3961 gatgcgcgca tcgagcgggt ccggcggcag gtcggcgcta cccgccccgg cctgcgcatg
     4021 cagcgccgac agcggcagca ggccggcaga cagcagtacg acgaaccggg cggagacccg
     4081 gcgcggaaat ctggacatga tcggctccgg tggcggatgg aaagcgcccc cgccccggcc
     4141 cggcatgggc ggcggacggc tgtcagcctt cggcgcgcag ccgcgccagc agttccgcgg
     4201 t
//
LOCUS       DAOQIE010000321         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12771_length_4176_cov_6.978403, whole genome shotgun sequence.
ACCESSION   DAOQIE010000321 DAOQIE010000000
VERSION     DAOQIE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12771_length_4176_cov_6.978403"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1471
                     /gene="gorA"
                     /locus_tag="PK144_15895"
     CDS             119..1471
                     /gene="gorA"
                     /locus_tag="PK144_15895"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNF67261.1"
                     /translation="MSRYDYDLITIGAGSGGLSVAERAAAYGRRCVIIEAGPLGGTCV
                     NVGCVPKKIMWFAAHHAHALADAAGFGFDVHVGGHDWAALKRARDGYVGRIVEWYGGW
                     LADQHIELIRGRAHCIDAHTVEVEGRRLSAEHLVIATGGLPKQPDIPGASFGIDSNGF
                     FALEARPARAVVVGAGYIAVELAGVLRALGTDTTLLLRKDTVLREFDPMLGETLMSAL
                     AADGVRILTRTEPQRIERRAGGSLHIERSHGDPIDTDCLLWAIGRAPATAGLNLEAAG
                     VALAEDGTIPVDDYQNTAAPGVYALGDVTGRAALTPVAIAAGRRLADRLFGGQPDRHL
                     DYGLIPSVVFTHPPLGTVGLTEPEARSRYGDEAVRVYTSRFTPMARAFSPERRETAMK
                     LVCTGPEQRVVGVHVIGDGADEMLQGFAVAVRMGATKRDFDDTVAIHPSSAEELVTMR
                     "
     gene            1523..1924
                     /locus_tag="PK144_15900"
     CDS             1523..1924
                     /locus_tag="PK144_15900"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNF67262.1"
                     /translation="MSPMTLLRCAVALAGAVLLPAAPVLADPIQDCRKEITREQPDVS
                     VCLSDRLRIAEAGLVSADRDKRKNMRELDQRLGGRSALQAFERSAQAFRNYRAAHCNW
                     WSVRSGRPDLEQKLACEIDLVELRMRTLLLE"
     gene            2053..2991
                     /locus_tag="PK144_15905"
     CDS             2053..2991
                     /locus_tag="PK144_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNF67263.1"
                     /translation="METFIRVTEIWIPTRDRTRLELGAGLYGPLEEFEALSRTMQFGY
                     DEGLPGKAWAAGHPVILKGFEGSYFQRTEAAQLAGLTCGIAVPVFAGDFLLAVMVLFC
                     GDDEAHVGAIEVWHNDPAVSHDMSLLDGYYGTAELFEWTSRRVSFRPGTGLPGMVWES
                     GMPVALDDLGDGNRFLREDCAVKVGINRGLGIPVSRNDDQHYVLTFLSALGTPIAHRF
                     EIWTPATDGTALLFDSGHCEASPELAEFYAEAHIGLGEGVLGKAWLSGLPGLSRDLPA
                     DPSIAARAALSAGLTEVVALPVIRNGQLKAIVAWYF"
     assembly_gap    2992..3091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3105..>4176)
                     /locus_tag="PK144_15910"
     CDS             complement(3105..>4176)
                     /locus_tag="PK144_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF67264.1"
                     /translation="PIREAGTLVPQLLVAAWLRRLALRKYAWAFGGLTQALAAAGMAI
                     VALAGDGPVGVASVLGLLLLLALGRGFSSIATKDVMGKTIAKSRRGTLMGWSDSVASV
                     LALVIGLTLPLLGERPERGLLAALLLLAAGGWLLNALCALTMREEPGATEGAVNAGAA
                     LLEGLQLLRADPAFVRFNLSRGLLLGSALSLPYLVVLAHEHSDADLAGLGVLIVASNL
                     GVFLSSPFWGRWADRSSRHVMAAAGALAGLTTAVALASDALPATLAGSLWYYGALYGV
                     LAAAHAGIQLGRKTWVVDLGGPKRALYVAMSNTLAGVLMLAAGALTGLIARFWGHHGA
                     LLLLTAMALAGAVSTLWLKDAE"
BASE COUNT          600 a         1517 c         1420 g          539 t
ORIGIN      
        1 ccgacactct acgccggcca cgcccggccg gtgcaggcag gcgggccgcg gccgccgcgg
       61 tcgctatgct tcggggccgc tgccggcaca tcggcagcgc acgcgaggaa accatccgat
      121 gagccgttac gactacgacc tgatcacgat cggcgccggc tccggcggcc tgtcggtcgc
      181 cgaacgcgcc gccgcttacg gccggcgctg cgtgatcatc gaggccgggc cgctcggcgg
      241 cacctgcgtc aacgtcggct gcgtgccgaa gaagatcatg tggttcgccg cccatcacgc
      301 gcacgcgctc gccgatgccg ccggcttcgg cttcgacgtg cacgtcggcg gtcacgactg
      361 ggcggcgctc aagcgcgcac gcgacggcta cgtcgggcgc atcgtcgagt ggtatggcgg
      421 ctggctcgcg gaccagcaca tcgagctgat ccgcggccgg gcgcactgca tcgatgcgca
      481 cacggtcgag gtcgagggcc ggcggctcag cgccgaacac ctggtgatcg cgaccggcgg
      541 cctgccgaag cagcccgaca tccccggcgc ctcgttcggc atcgactcga acggcttctt
      601 cgcgctcgaa gcccggccgg cgcgcgcagt cgtcgtcggg gccggttaca tcgccgtcga
      661 actcgccggc gtgctgcgcg cgctcggcac cgacacgacg ctgctgctgc gcaaggacac
      721 ggtgctgcgc gagttcgatc cgatgctcgg cgaaacgctg atgtcggcgc tcgcggccga
      781 cggcgtgcgg atcctgaccc gcaccgagcc gcagcgcatc gaacggcgcg ccggcggcag
      841 cctgcacatc gagcgcagcc acggcgatcc gatcgacacc gactgcctgc tgtgggcgat
      901 cggccgggcg ccggccaccg ccggcctgaa cctcgaagcc gccggcgtgg cgctcgccga
      961 agacggcacg atcccggtcg atgactacca gaacaccgcc gcacccggcg tctacgcgct
     1021 cggcgatgtc accggccgcg cggcgctgac cccggtcgcg atcgccgccg ggcgccggct
     1081 cgccgaccgg ctgttcggcg gccagccgga tcgccacctc gactacgggc tgatcccgag
     1141 cgtcgtgttc acgcacccgc cgctcggcac cgtcggcctg accgagcccg aggcgcgcag
     1201 ccggtacggc gacgaggccg tgcgcgtcta caccagccgc ttcacgccga tggcacgggc
     1261 gttctcgccc gagcggcgcg agacggcgat gaagctcgtc tgcaccggcc cggagcagcg
     1321 cgtcgtcggc gtgcacgtga tcggtgacgg tgccgatgaa atgctgcagg gtttcgcggt
     1381 cgccgtgcgc atgggtgcga ccaagcgcga cttcgacgac acggtcgcga tccacccgag
     1441 cagcgccgag gaactggtga cgatgcgctg aggccggcgc ccgccgttcc gccccccatc
     1501 ccgcccggag gaaaccttgc tgatgagtcc gatgaccctg ctgcgctgcg ccgtcgcgct
     1561 cgccggcgcc gtgctgctgc cggccgcgcc ggtgctggcc gacccgatcc aggactgccg
     1621 caaggagatc acgcgcgagc agcccgacgt gagcgtctgc ctgtccgatc gcctgcgcat
     1681 cgccgaggcc ggtctggtat cggccgatcg cgacaagcgc aagaacatgc gcgaactcga
     1741 ccagcgcctc ggcggacgct cggcgctgca ggccttcgaa cgctcggcgc aggccttccg
     1801 caactaccgc gccgcgcact gcaactggtg gtcggtgcgc agcggacgcc ccgatctgga
     1861 gcagaagctc gcctgcgaga tcgatctggt cgaactgcgg atgcgcacat tgctgctcga
     1921 atagcccgtg cccgccccgt catcgcgcat tgcgcatggc ggcatgactc gtgcttgtcg
     1981 cgctcgcaca cccgcgcgcg ccggccagaa gccggcgccc ggacatgacg acgacttgac
     2041 ggggaataca gcatggaaac cttcatccgg gtcactgaaa tctggatccc gacccgggac
     2101 cgcacgcggc tggaactcgg cgccggactg tacggcccgc tggaagaatt cgaggcgctg
     2161 agccgcacga tgcagttcgg ctacgacgag ggcctgcccg gcaaggcctg ggcggccggc
     2221 catccggtga tcctgaaggg cttcgaaggc agctacttcc agcgcaccga ggccgcgcag
     2281 ctcgccggcc tgacctgcgg catcgcggtg ccggtgttcg ccggtgactt cctgctggcg
     2341 gtgatggtgc tgttctgcgg cgacgacgag gcccacgtcg gcgccatcga ggtctggcac
     2401 aacgatccgg ccgtctcgca cgacatgtcg ctgctcgatg gctactacgg cacggccgag
     2461 ctgttcgagt ggacctcgcg gcgcgtgagc ttccgccccg gcaccggcct gcccggcatg
     2521 gtctgggaat ccggcatgcc ggtggcgctc gatgatctcg gcgacggcaa ccgcttcctg
     2581 cgcgaggact gcgcggtcaa ggtcggcatc aaccgcggcc tcggcattcc ggtctcgcgc
     2641 aacgacgacc agcactacgt gctgaccttc ctgtcggcgc tcggcacgcc gatcgcgcac
     2701 cgcttcgaga tctggacacc ggccaccgac ggcacggcgc tgctgttcga ttccggccac
     2761 tgcgaggcca gccccgaact cgccgagttc tacgccgagg cgcacatcgg actcggcgag
     2821 ggcgtgctcg gcaaggcctg gctgagcggc ctgccggggc tgagccgcga cctgccggcc
     2881 gacccgagca tcgctgcgcg cgcggccctg agcgccgggc tgaccgaggt cgtcgcgctg
     2941 ccggtgatcc gcaacggcca gctgaaggcg atcgtcgcct ggtacttctg annnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggatgccg gttctcactc ggcatccttc
     3121 agccacagcg tgctcaccgc gccggcgagc gccatcgccg tcagcagcag cagcgcgccg
     3181 tggtggcccc agaagcgggc gatcagcccg gtcagtgcac cggcagcgag catcagcacg
     3241 ccggccagcg tgttcgacat cgccacgtac agcgcgcgct tcggcccgcc gaggtcgacg
     3301 acccaggtct tgcggccgag ctggatgccg gcgtgcgcgg cggcgagcac gccgtacagc
     3361 gcgccgtaat accagagcga gccggcgagc gtcgccggca gcgcatcgct tgccagcgcc
     3421 accgcggtcg tcagcccggc cagcgcaccg gcggcggcca tcacgtgccg gctcgaacgg
     3481 tcggcccagc ggccccagaa cgggctgctc aggaacacgc cgaggttgct ggcgacgatc
     3541 agcacgccga ggccggcgag atcggcgtcg ctgtgctcgt gcgcgagcac gaccagatac
     3601 ggcagcgaca gcgcgctgcc gagcagcagg ccgcggctca ggttgaagcg cacgaaagcc
     3661 ggatcggcgc gcagcagctg caggccttcg agcagcgccg cgccggcgtt gaccgcgcct
     3721 tcggtcgcgc ccggctcctc gcgcatcgtc agcgcgcaga gcgcgttcag cagccagccg
     3781 ccggccgcga gcagcagcag cgccgcgagc aggccgcgct cggggcgttc gccgagcagc
     3841 ggcagcgtca ggccgatcac gagggccagc acgctcgcga cgctgtcgct ccagcccatc
     3901 agcgtgccgc ggcgggattt cgcaatcgtc ttgcccatca cgtccttggt cgcgatcgag
     3961 ctgaagccgc gcccgagcgc gagcagcagc agcagcccga gcacgctggc gacgccgacc
     4021 ggcccgtcgc cggcgagcgc gacgatcgcc atgccggccg cggccagcgc ctgcgtcagg
     4081 ccgccgaagg cccacgcgta tttgcgcagc gccagccgcc gcagccacgc cgccaccagc
     4141 agctgcggca ccagcgtgcc ggcctcgcgg atcggc
//
LOCUS       DAOQIE010000322         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12851_length_4158_cov_6.964904, whole genome shotgun sequence.
ACCESSION   DAOQIE010000322 DAOQIE010000000
VERSION     DAOQIE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12851_length_4158_cov_6.964904"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PK144_15915"
     CDS             <1..1338
                     /locus_tag="PK144_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNF67265.1"
                     /translation="SVDESLLTGESLPQARTVGDAVIGGSVNVESPLRVRVERVGADS
                     VLSSIVRLLDRAQSEKPRIAALADRIAGHFVAVLLLVSLLVWAWWWQHEPARAFPIVL
                     AILVVTCPCALGLATPAAITAATGALTRIGLLTTRGHALETLARATHVVFDKTGTLTI
                     GRLRLVDTVAVRAGLTADEALELAAALECGSEHPVGQAIVDAARGTRRALDLRNTPGH
                     GVEGTIDGRVYRIGKPAAAAEPPQASDAEASTWVVLADAEGTLAWLRFADRLRPDAPE
                     TVGRLRAAGLEVWLLSGDRPAAVAAIAAEAGIAHAEGGLLPADKLARIEALQQGGAVV
                     AMVGDGINDAPVLAAASVSIAMGSGTQLAHASADMILLSSGLGHLVDGVTTARRTLAI
                     IRQNLAWSIGYNVLAVPAAAAGWIAPWLSAIGMSASSLLVVVNALRLRARRRA"
     gene            1351..1602
                     /gene="ccoS"
                     /locus_tag="PK144_15920"
     CDS             1351..1602
                     /gene="ccoS"
                     /locus_tag="PK144_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNF67266.1"
                     /translation="MEILYLLIPLAIAIMLVAVWAFVWSVKSGQFDDLEGPAHRILMD
                     DDDPMIPRPDQPADKPSGPSRPSASPEASRPSAARDGDA"
     gene            1741..2721
                     /gene="birA"
                     /locus_tag="PK144_15925"
     CDS             1741..2721
                     /gene="birA"
                     /locus_tag="PK144_15925"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HNF67267.1"
                     /translation="MPARAAQLLERLADGGFRSGEVLAAELGISRAAVWKLVRGWQAR
                     GLDIQAVRGRGYRLARPLELLDAGLIQARLDETAAARLGPLEVLAETDSTNTRLAQLA
                     RSGLPAGAVCLAESQRAGRGRLGRQWISPYAANLYLSVLWRFALPPAALAGLSLAIGV
                     ALARSLERFGVGGHGLKWPNDLLWDARKLGGVLLEFGGESEGCSHVIIGVGVNVAMPE
                     SAGGTIDQPWIDLATVLGRTHVSRNRLAALLLSEIAIACAAFGESGLDSFQSDWQRWD
                     AVAGRPVRLLWPGHVVEGIARGIAADGELQLDTASGRRSFAIGELSLRPA"
     gene            2718..3473
                     /locus_tag="PK144_15930"
     CDS             2718..3473
                     /locus_tag="PK144_15930"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNF67268.1"
                     /translation="MTARLLIDAGNTRLKWRLDAEDGPRVAGCLVHRDRPLAEALAEL
                     PDALARPSAIWCGSVAGNHFDAALDAALRARWSLSPTFVQATAAAGGIVSAYAEPARM
                     GVDRWLAMIGARARTRRACCVIDAGTAVTVDALAADGRHLGGTILPGLQLMRTALYGN
                     TRRIPAGPVPATPSGLGRDTAACVAAGVRHAVVGGITGSVQSAGAGAGEGLHCLLTGG
                     DGAFLAPLLPFAVELVPDLVLDGLARLAGSAGA"
     gene            3535..3610
                     /locus_tag="PK144_15935"
     tRNA            3535..3610
                     /locus_tag="PK144_15935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Thr,seq:tgt)
     gene            3684..3768
                     /locus_tag="PK144_15940"
     tRNA            3684..3768
                     /locus_tag="PK144_15940"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3718..3720,aa:Tyr,seq:gta)
     gene            3848..3921
                     /locus_tag="PK144_15945"
     tRNA            3848..3921
                     /locus_tag="PK144_15945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3880..3882,aa:Gly,seq:tcc)
     gene            3945..4020
                     /locus_tag="PK144_15950"
     tRNA            3945..4020
                     /locus_tag="PK144_15950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3978..3980,aa:Thr,seq:ggt)
BASE COUNT          552 a         1461 c         1465 g          680 t
ORIGIN      
        1 agcgtcgatg aatcgctgct gaccggcgag agcctgccgc aggcccgcac ggtcggcgat
       61 gccgtgatcg gcggctcggt caacgtcgaa agcccgctgc gcgtgcgcgt cgagcgcgtc
      121 ggggccgaca gcgtgctgtc gtcgatcgtg cgcctgctcg atcgcgcgca gagcgagaag
      181 ccgcgcatcg ccgcgcttgc cgaccgcatc gccgggcact tcgtcgccgt gctgctgctg
      241 gtgtcgttgc tggtctgggc ctggtggtgg cagcacgaac cggcgcgcgc cttcccgatc
      301 gtgctcgcga tcctggtcgt gacctgtccg tgcgcgctcg gtctggcgac accggccgcg
      361 atcaccgccg cgaccggcgc gctgacgcgc atcggcctgc tgaccacgcg cggccacgcg
      421 ctcgaaaccc tggcgcgcgc gacgcacgtg gtgttcgaca agaccggcac gctgaccatc
      481 ggccggctgc gcctggtcga taccgtggcg gtgcgcgcgg ggctcacggc cgatgaggcg
      541 ctcgaactcg cggcagcgct cgaatgcggc tccgaacacc cggtcgggca ggcgatcgtc
      601 gatgccgcgc gcggcacgcg gcgcgcgctc gatcttcgca acaccccggg ccacggcgtc
      661 gagggcacga tcgacggccg cgtctaccgg atcggcaagc cggctgccgc agccgagccg
      721 ccgcaggcaa gcgatgccga agcctcgacc tgggtcgtgc tcgccgatgc cgaaggaacc
      781 ctcgcgtggc tgcgcttcgc cgaccgcctg cgtccggatg cccccgagac ggtcgggcgg
      841 ctgcgggctg ccgggctcga agtctggctg ctgtccggcg accggccggc ggcggttgcg
      901 gccatcgccg ccgaggccgg catcgcccac gccgaaggcg ggctgctgcc ggcggacaag
      961 ctcgcgcgca tcgaggcact gcagcagggc ggggcggtgg tggcaatggt cggcgacggc
     1021 atcaacgatg cgccggtgct cgcggcggcc agcgtctcga tcgcgatggg ttcgggcacg
     1081 cagctcgcgc atgcctcggc cgacatgatc ctgctgtcct cggggctcgg acatctcgtc
     1141 gacggcgtca ccaccgcgcg gcgcacgctg gcgatcatcc gccagaacct cgcctggtcg
     1201 atcggctaca acgtgctggc cgtgccggcc gccgccgccg gctggatcgc cccctggctg
     1261 tcggcgatcg gcatgtcggc gagttcgctg ctcgtcgtcg tcaatgcgct gcgcctgcgc
     1321 gcgcgccgcc gggcctgagg gagcgccagc atggaaatcc tgtacctgct gatcccgctc
     1381 gcgatcgcga tcatgctggt cgcggtgtgg gccttcgtct ggtcggtgaa gtccggccag
     1441 ttcgatgacc tcgaaggtcc ggcgcaccgc atcctgatgg acgacgacga cccgatgatc
     1501 ccgcgtcccg atcagcccgc cgacaaacct tccggaccct ccagaccttc cgcttccccg
     1561 gaagcttccc ggccgtccgc tgcgcgtgac ggcgatgcct gaagtatcat ccgaccctca
     1621 ccgcccaagg agtcatgtcc atgttcacgt tcgagaactt ttccctggtg atcatcattg
     1681 ccctgatcat cctgtcctgg ctgagcttct tctcggtcat cctctgacgg caggccggtc
     1741 gtgccggcac gcgccgcgca gctgctggag cgcctcgccg atggcggctt ccgttccggc
     1801 gaggtgctgg cagccgaact cggcatcagc cgggcggcgg tctggaagct cgtgcgcggc
     1861 tggcaggcgc gcggcctcga catccaggcc gtgcgcgggc gcggctaccg gctggcccgt
     1921 ccgctcgaac tgctcgacgc cgggctgatc caggcccggc tcgacgagac cgccgcggcc
     1981 cgcctcgggc cgctcgaagt gctcgccgaa accgattcga ccaacacccg gctcgcgcag
     2041 ctggcgcgca gcggcctgcc ggcgggcgcg gtctgtctgg ccgaaagcca gcgcgccggc
     2101 cgcggccggc tcggccggca gtggatctcg ccgtacgcag ccaacctgta tctgtcggtg
     2161 ctgtggcgct tcgcgctgcc gcccgccgcg ctcgccgggc tgtcgctcgc gatcggcgtg
     2221 gcactggcgc gttcgctcga acgcttcggc gtcggcggcc acggcctcaa gtggccgaat
     2281 gacctgctgt gggacgcccg caagctcggc ggcgtgctgc tcgaattcgg cggcgagagc
     2341 gaaggctgca gccacgtcat catcggtgtc ggcgtgaacg tcgcgatgcc ggagtccgcc
     2401 ggcggcacga tcgaccagcc gtggatcgac ctggcgacgg tgctcgggcg cacgcatgtc
     2461 tcccgcaacc ggctcgcggc gctgctgctg tccgagattg ccatcgcctg cgcggccttc
     2521 ggcgagtcgg ggctcgacag cttccagtcc gactggcagc gctgggacgc ggtcgccggc
     2581 cggccggtgc ggctgctgtg gccgggtcac gtcgtcgagg gcatcgcacg cggcatcgcg
     2641 gccgacggcg aactgcagct cgataccgcc agcggccggc gcagcttcgc gatcggcgaa
     2701 ctgagcctga ggccggcatg acggcccggc tgctgatcga tgccggcaac acccggctga
     2761 agtggcgcct cgatgccgaa gacgggccgc gggtcgccgg ctgcctcgtg catcgcgacc
     2821 gcccgctggc cgaggctctt gccgaactcc cggacgcgct cgcacggcct tcggcgatct
     2881 ggtgcggcag cgtcgccggc aaccacttcg acgccgcgct ggatgctgcg ctgcgggcac
     2941 gctggagcct gtcgccgacc ttcgtgcagg cgacggccgc ggccggcggc atcgtcagtg
     3001 cctatgccga gccggcgcgc atgggggtcg accgctggct ggccatgatc ggtgcgcgcg
     3061 cacgcacacg gcgggcctgc tgcgtgatcg atgccggcac ggccgtgacc gtcgatgcgc
     3121 tcgccgcgga cggccggcat ctgggcggca cgatcctgcc cggcctgcag ctgatgcgta
     3181 ccgcgctgta cggcaacacc cgccgcatcc ccgccgggcc ggtgccggcc acgccctcag
     3241 gtctcggtcg cgacacggcc gcctgtgtgg ccgccggcgt gcggcatgcc gtcgtcggcg
     3301 gcatcaccgg cagcgtccag agcgccggag ccggggcggg cgagggtctg cactgtctgc
     3361 tgaccggtgg cgatggcgcc ttcctcgcgc cgctgctgcc gttcgcggtc gagctcgtgc
     3421 cggatctggt gctcgacggg ctggcgcggc tggccggttc cgcaggcgcc tgagcggttt
     3481 gcgggcgctc gccggcttcg tctacactgc gcgccccgtg gcgttgccgg gcacgctggc
     3541 gtagctcagt cggtagagca gctgtcttgt aaacagcagg tcgtccgttc gatccggacc
     3601 gccagcacca gatatccgga agaagtcgca ggaagtagtg ttgacgggtc cggaagtgca
     3661 cggcagaatg cgcgtcctgt tttggagggg ttcccgagcg gtcaaaggga gcagactgta
     3721 aatctgccgg ctcagccttc gaaggttcga atccttcccc ctccaccaaa tttgtcttga
     3781 tccggttttg accagttgcc atccgttgca gtcagcgtgg cggcgggggc gcaaaagctc
     3841 tggcagggcg ggtgtagttc aatggtagaa cctcagcctt ccaagctgat ggtgtgggtt
     3901 cgattcccat cacccgctcc aatcggtcgc tgtttcgttt caaggcccat gtagctcagt
     3961 cggtagagca cgtccttggt aaggacgagg tcaccagttc gaatctggtc atgggctcca
     4021 ccgcctgttc cctgtctgtt cgtctcaaga aatcgtcaag catcccgcgt gatcatcggg
     4081 gagagttcgc aatggccaaa gagaaattcg agcgcaagaa gccgcacgtg aacgtgggca
     4141 cgatcggtca cgtcgacc
//
LOCUS       DAOQIE010000323         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12860_length_4156_cov_6.846867, whole genome shotgun sequence.
ACCESSION   DAOQIE010000323 DAOQIE010000000
VERSION     DAOQIE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12860_length_4156_cov_6.846867"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1017
                     /locus_tag="PK144_15955"
     CDS             454..1017
                     /locus_tag="PK144_15955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014598338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNF67269.1"
                     /translation="MSDRPLTFLSITGSLRRGSYNAAIARALPELAPDGIRVVAAPSL
                     RDIPLYDADLQAEGFPAAVTALGDAIRAADGVVIVSPEYNYSVPGVLKNAIDWISRLP
                     DQPFAGKPLAIQSASMGVLGGARMQYHLRQIFVFLDARILNKPEIMVGQAQNKIDADG
                     QLTDAGTREFLGKQLAALAALARQMAG"
     gene            complement(1027..1647)
                     /locus_tag="PK144_15960"
     CDS             complement(1027..1647)
                     /locus_tag="PK144_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNF67270.1"
                     /translation="MTEPEPGKPRLLVVAGPNGSGKTTVTEQVLKHRWFDGCEYINPD
                     NIAEQQYGGWNSAEAVLQAANHAQALRERCLAEGRDMAFETVFSADDKVQFIERALAA
                     GYFVRVFFVSTRHPSLNAARITYRVMNGGHDVSIAKIIARYRRSIVNCTLIARRVDRL
                     YVYDNSVDGAEARLMFRARYGELAKVYAGPIERWADSIHTALRAPA"
     gene            complement(1640..1858)
                     /locus_tag="PK144_15965"
     CDS             complement(1640..1858)
                     /locus_tag="PK144_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67271.1"
                     /translation="MIQSAACRLDPLANDCEPTDEQLEMIMAAAARSAREIRARVDAE
                     LYRQIAVELEAAKARARARGSIFVQDHD"
     gene            complement(1955..2893)
                     /gene="msrP"
                     /locus_tag="PK144_15970"
     CDS             complement(1955..2893)
                     /gene="msrP"
                     /locus_tag="PK144_15970"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNF67272.1"
                     /translation="MLIRRPSDLRPSEITSPELFHGRRRFLQGALAAGAAAAFGLPAV
                     ASAGEKLPNVVKSPYRVDEPLTPYDAVTEYNNFYELGTGKDDPQRNAARLKPKPWTVE
                     IAGACEQPGRVGFDDLIAGLALEERVYRHRCVEAWSMVVPWVGVPLGQVLAKYKPLSS
                     AKYVAFTTLMDPQQFPGQRLPVLEWPYREGLRMDEALHPLTILAVGVYGEVLPNQNGA
                     PLRLVVPWKYGFKGIKSIVKIEFVEHEPRTSWNIAAPDEYGFYANVNPAVDHPRWSQK
                     RERRIGDWLKRDTLAFNGYGEQVASLYSGMDLRKSF"
     gene            3110..3331
                     /locus_tag="PK144_15975"
     CDS             3110..3331
                     /locus_tag="PK144_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67273.1"
                     /translation="MRENFSEDEESLDDDTEHESPAVVPTPPPAPSGPGSKAWRSIER
                     HRELRELRRHLDDFQFEDDPKYDPRNLPL"
     gene            3473..3751
                     /locus_tag="PK144_15980"
     CDS             3473..3751
                     /locus_tag="PK144_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF67274.1"
                     /translation="MIASFRDKDTCRLMEGEPVPRLAACRKAALRKLDMLAAAHVLQD
                     LRQPPGNRLEALQGNRAGQHSIRINDQWRLCFVWRDGHAHEVEIVDYH"
     gene            3761..4042
                     /locus_tag="PK144_15985"
     CDS             3761..4042
                     /locus_tag="PK144_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNF67275.1"
                     /translation="MRITTHPGEVLREEFLIPHGLSANALALALHVPATRINDIVRER
                     RAITADTAARLSRYFGTTAEFWLNLQTAHDLSAVLSKHRAEIERIQPVA"
BASE COUNT          696 a         1421 c         1363 g          676 t
ORIGIN      
        1 acgatgacga actccgccca cagcccgtag cggtcatcgc cccaggcatc ggcgaacggc
       61 ggcggaaagg tttcgggcca gcgggcggct tcggcggcgg ggttcagcag catggtgtgt
      121 cctgtctgtc gtcatcggcc tgcgcgcgca gcggcaccgg cacgggcttg tccacgtcgt
      181 ctgccatcac ccgatctcct gcaaaccccg gtgcagcgta gaagccgccg tatcgggcgg
      241 cagtgcatcg gctgattttc cggcaaagcc accgccggag ctgcaccgga tcgccggccc
      301 ggcgccgctg ctaccttcaa tgccgctgaa caactcccgc gaatccctcc gctatcccgc
      361 cgcgcaccgc ctgcgcgagc atcccgggca cgttttcccg ggcattccgc cccttccctg
      421 cccttttcca ccgaaacgga gcctgccccc gacatgagcg atcgtccgct gaccttcctg
      481 agcatcaccg gcagcctgcg ccgcggctcg tacaacgccg ccatcgcccg cgccctgccc
      541 gaactggcgc cggacggcat ccgcgtcgtc gccgcgccgt cgctgcgcga catcccgctg
      601 tacgacgccg acctgcaggc cgaaggcttt ccggccgccg ttaccgcgct cggcgatgcc
      661 atccgcgccg ccgacggcgt cgtcatcgtc tcgcccgaat acaactactc ggtgcccggc
      721 gtgctgaaga acgccatcga ctggatctcg cgcctgcccg atcagccgtt cgccggcaag
      781 ccgctggcga tccagagcgc gtcgatgggc gtgctcggcg gcgcgcgcat gcagtatcac
      841 ctgcgccaga tcttcgtctt cctcgacgcc cgcatcctca acaaacccga aatcatggtc
      901 ggtcaggcgc agaacaagat cgacgccgac ggccagctca ccgacgccgg cacgcgcgaa
      961 ttcctcggca agcaactggc ggcgctggcg gcgctggcgc ggcagatggc gggctgaagg
     1021 cgcggatcag gccggcgcgc gcagcgccgt gtggatgctg tcggcccagc gttcgatcgg
     1081 gccggcatag accttcgcca gctcgccgta gcgggcccgg aacatcagcc gggcctcggc
     1141 gccgtcgacc gagttgtcgt agacgtacag ccggtcgacg cggcgggcga tcagcgtgca
     1201 gttgacgatc gagcgccggt agcgcgcgat gatcttggcg atcgatacat catggccgcc
     1261 gttcatgacc cgataggtga tgcgggcggc gttgagggac gggtggcggg tcgagacgaa
     1321 aaacacccgg acgaaatatc cggcggccag cgcccgctcg atgaactgca ccttgtcatc
     1381 ggccgagaac accgtctcga aggccatgtc gcggccttcg gcgaggcagc gctcgcgcag
     1441 cgcctgcgcg tggttcgccg cctgcagcac ggcttcggcc gaattccagc cgccgtactg
     1501 ctgctcggcg atgttgtcgg gattgatgta ttcgcagcca tcgaaccagc ggtgcttcaa
     1561 cacctgctcg gtgaccgtgg tcttgccgga accgttcggt ccggcgacga ccagcaagcg
     1621 tggcttgccg ggttcaggtt cagtcatggt cctgcacgaa gatgctgccg cgggcgcggg
     1681 cgcgggcctt ggcggcttcc agctcgacgg cgatctgccg gtaaagctcg gcatcgacgc
     1741 gcgcccgaat ctcgcgggcc gagcgggcag cggcagccat gatcatttcg agctgctcgt
     1801 cggtcggctc gcagtcgttg gcgagcgggt cgaggcggca ggcggcggac tggatcattt
     1861 cggtaacctc cggatggcag gtgcctgacg gaaaaagcct gtcgggcagt gtatgcctgt
     1921 cggacaggcg gtcaatcgtc gccggcggcc ggcctcagaa cgacttgcgc aggtccatgc
     1981 ccgagtacag cgaggcgacc tgctcgccgt agccgttgaa cgccagcgtg tcgcgcttca
     2041 gccagtcacc gatgcgccgc tcgcgcttct ggctccagcg cgggtgatcg acggccggat
     2101 tgacgttggc atagaagccg tactcgtcgg gcgcggcgat gttccacgag gtgcgcggct
     2161 cgtgctcgac gaactcgatc ttcacgatcg acttgatgcc cttgaagccg tacttccacg
     2221 gcacgaccag ccgcagcggc gcgccgttct ggttcggcag cacctcgccg tagacgccga
     2281 cggcgaggat cgtcagcgga tgcagcgcct cgtccatgcg caggccctcg cggtacggcc
     2341 actccagcac cggcaggcgc tggccgggaa actgctgcgg gtccatcagc gtcgtgaacg
     2401 ccacgtactt cgcgctcgac agcggcttgt acttcgcgag cacctgcccg agcggcacgc
     2461 cgacccaggg cacgaccatc gaccaggcct cgacgcagcg gtggcggtag acgcgctcct
     2521 ccagcgccag tccggcgatc agatcgtcga agccgacgcg accgggctgc tcgcaggcgc
     2581 cggcgatctc gacggtccac ggcttcggct tcaggcgtgc ggcgttgcgc tgcggatcgt
     2641 ccttgccggt gccaagctcg tagaagttgt tgtactcggt gacggcgtcg tacggcgtca
     2701 gcggctcgtc gacgcgatag ggacttttga cgacgttcgg cagcttctcg ccggcgctcg
     2761 cgaccgccgg caggccgaac gcggcggccg caccggcagc gagcgccccc tgcaggaagc
     2821 ggcggcggcc gtggaacagt tcgggcgacg tgatctcgga cgggcgcaga tcggacggac
     2881 ggcggatcag catgacggcg actcccggat gtggacgacc ggactgctta cgacgctgtg
     2941 gcggccgtgg attccatcga aaaaactttg tctgtgatca aaaattctga tttctgttga
     3001 ccgttgaaac tccgtataaa ccgcgcaccc tgaaaccggg ggtcggtgac cgtggtgtcc
     3061 ggcccggacc gaaggttctg ctgcagcagc gtgcaggagt gacttgaaca tgcgtgaaaa
     3121 cttcagcgaa gacgaggaaa gcttggacga cgacacggaa cacgagtcgc cggccgtggt
     3181 gccgacgccc ccgcccgcgc cgtcgggccc cggctccaag gcctggcgat ccatcgagcg
     3241 gcatcgcgaa ctgcgcgaac ttcgccgcca cctcgacgac ttccagttcg aggacgatcc
     3301 caagtacgac ccgaggaacc tgccgctctg atccggcacg caaccgtttt cccccgctga
     3361 acccgcccgg gcggtgcagg caactgcacc gcccggagtg tttcggggca ggatcggggt
     3421 ggcagaggtg ctttgacgcg gcgcgtttaa cgcgaaacgg tataagctgt cgatgatcgc
     3481 gtcattcagg gacaaggaca cctgccggct catggagggc gagcccgtgc cccgccttgc
     3541 agcctgccgg aaagccgcct tgcgcaagct cgacatgctc gcggctgccc atgtccttca
     3601 ggatttgcgg caaccaccgg gtaaccgtct ggaagccttg cagggtaacc gggccggaca
     3661 gcacagcatc cggatcaatg accagtggcg actgtgcttc gtctggcgcg acggacacgc
     3721 gcacgaggtc gaaatcgtcg attaccactg aggtcatgcc atgaggatca cgacacatcc
     3781 gggcgaagtg ctgcgcgaag agttcctgat tccacacggc ctgagtgcca acgccctggc
     3841 acttgccctg catgttccgg ccacgcgcat caacgacatc gtgcgcgagc gtcgcgccat
     3901 caccgccgac acggccgccc ggctgtcccg ctatttcggc accacggccg agttctggct
     3961 gaacctgcag accgctcatg atctgtccgc ggtcctcagc aagcatcgcg ccgagatcga
     4021 acggattcag ccggtcgcct gaatgaaggc ggctgaaccc gccggctgac ctggaagccc
     4081 gaacagcaca ccttcatcgc gcaaaacgaa acggcccgga cgttgccgtc cgggccgctg
     4141 aggtgtctgg tgggcg
//
LOCUS       DAOQIE010000324         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12881_length_4152_cov_4.846229, whole genome shotgun sequence.
ACCESSION   DAOQIE010000324 DAOQIE010000000
VERSION     DAOQIE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12881_length_4152_cov_4.846229"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..910)
                     /locus_tag="PK144_15990"
     CDS             complement(17..910)
                     /locus_tag="PK144_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF67276.1"
                     /translation="MKRLLIVGCGDVAWRALPRLRNRYRVFSLLRDPLQAPRWRGAGA
                     TPIIADLDDPASLHRIRGLADDVLHLAPPPDRSDRDARTQALLAALERGKSLPQRLVY
                     VSTSGVYGDCGGALVDETRPSNPLSARGARRVDAERQLRAFGRRTGAIVSILRAPGIY
                     AADRLPLERLKKGLPALCDEDDVFTNHIHADDLAAACAAALDHGRAGRSYNVSDDSEL
                     RMGEYFDHVADAFGLPRPPRISRAEAERVMSPVQLSFMRESRRLVNRRLKEELGLRLA
                     YPSVDDGIRAALNETRNPSCS"
     gene            964..1995
                     /locus_tag="PK144_15995"
     CDS             964..1995
                     /locus_tag="PK144_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNF67277.1"
                     /translation="MSFTVTIQPSGHTLSTEAGESILEAALRQGLTLPYGCRDGACGA
                     CRGKVLSGEVDHGKAQDHALSQADREQGLALFCCASPRSDLVIESREVRKAGDIPVKT
                     LPVRVQSLERAAPDVMIVKLKLPASERLQFLAGQYVDILLKDGRRRSFSLANAPHDDA
                     LLELHIRHVPGGLFTEQVFTQMKEKDILRINGPHGTFFLREDSPKPIVMIAGGTGFAP
                     IKSVVEHAVAESCERPITVYWGGRQRIDLYALDVPERWASQSNIRFVPVLSEPPAEDS
                     WTGRRGFVHAAAMADFPDLSGHQVYACGSPAMIAAAKRDLVDRCKLPEDEFFADSFDF
                     ANDKHQEPA"
     gene            1992..3164
                     /locus_tag="PK144_16000"
     CDS             1992..3164
                     /locus_tag="PK144_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNF67278.1"
                     /translation="MSLFMTRQPLINRQGKIIATRLRLNATAGESSATVVDALNGLND
                     CWPQSDRSIFVNFDGVAGDMGFLDWIAPANVTFELNGAAFAGDDAAAFAQALKDADLS
                     LCYDFDGHAAQAFGSGTKFRFVGFDATRFSAAQLKALLAKTQPFGIPVAFNVDGQQEF
                     KACMDAGMSAAAGWFVKQPASAPAKQLNASQAHIVRVLNLVRKNAEVGQIESALKQDV
                     ALSYKLLRYINSAGFGLSCEVQSFKHAVTILGYDKLNKWLSLLLVTASKDPMAPTLMH
                     TALTRARLMEQLGHGLVDKSEYDNLFITGAFSMLDLLLGVTMEQALDAMHLPEPITDA
                     LLGNAGIYAPFLALALACEGGEVASLAEQAGMLGLTGEQLNRAQLAALSFADTMES"
     gene            complement(3238..3963)
                     /locus_tag="PK144_16005"
     CDS             complement(3238..3963)
                     /locus_tag="PK144_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF67279.1"
                     /translation="MSNGIEPLLIVEGLRVSYGGIQAVRGITFHINPGETVALIGANG
                     AGKTSTLKALARMLDAAGGSVRYQGKEISRLPSHHLVREGIALVPEGRGVFPRLTIAE
                     NLAMGAFCRDDKAEIARDTDHVYGLFPRLKEREAQLAGTLSGGEQQMLAIGRALMGRP
                     KLLLLDEPSMGLAPIMVQKIFEVVRTVAAEGMTILLIEQNAKLALETSQRGYVMESGE
                     ITLTDEAAKLLHDPKVREAYLGE"
     gene            complement(3960..>4152)
                     /locus_tag="PK144_16010"
     CDS             complement(3960..>4152)
                     /locus_tag="PK144_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF67280.1"
                     /translation="RLLIEGIRADGVTILLIEHDVKLVMGLCDRVAVLDYGALVIEDV
                     PSVVQHDPRVIEAYIGVAA"
BASE COUNT          734 a         1386 c         1326 g          706 t
ORIGIN      
        1 atgtgcaggg ccttgatcac gagcatgacg gattccttgt ttcgttgagg gcggcacgga
       61 tgccgtcgtc gaccgagggg taggcgaggc gaagccccag ttcttccttg aggcgccgat
      121 tgacgagccg gcgcgattct cgcatgaagg aaagctgcac gggcgacatc acgcgttcgg
      181 cctcggcccg cgaaatcctt ggcgggcggg gcaggccgaa ggcgtcggcg acgtggtcga
      241 agtactcgcc catgcgcagt tccgaatcgt cgctgacatt gtagctgcgg ccggcgcggc
      301 catgatccag tgcggcggca caggccgcgg cgagatcgtc ggcgtggatg tggttcgtga
      361 acacgtcgtc ctcgtcacac agggcgggca gccccttctt caggcgctcg agcggcaggc
      421 ggtcggcggc atagatgccc ggcgcacgca ggatggaaac gatggcgccg gtacggcgcc
      481 cgaaggcccg caactggcgc tcggcatcga cgcggcgggc gccgcgggcg ctgagcggat
      541 tcgacgggcg tgtttcgtcg accagggcgc cgccacagtc gccgtaaacc ccgctggtgc
      601 taacgtagac gaggcgctgt ggtagacttt ttcctcgctc gagtgccgcc agaagcgcct
      661 gcgtgcgggc atcccggtca ctccggtcgg gtggcggcgc aaggtgcagg acgtcgtcgg
      721 cgaggccacg gatccgatgc aggctggccg ggtcatcgag gtcggcaatg atcggcgtcg
      781 caccggcgcc acgccagcgt ggcgcctgca gcggatcacg caacagggag aatacgcggt
      841 agcggtttcg cagccggggc agggcccgcc aggcaacgtc gccacaaccg acgatcagaa
      901 gtcttttcac cggcgaattg tagcaatccg gcgccgcgtg caggcaagca acgaagggga
      961 atcatgagct tcaccgtcac catccagcca tcgggccaca cgctgagcac cgaggccgga
     1021 gaatccattc tcgaagcggc cctgcgccag ggcctgacgc tgccttacgg ttgccgcgat
     1081 ggcgcctgcg gcgcctgtcg cggcaaggtc ctgagcggcg aggtcgatca cggcaaggcg
     1141 caggaccatg ccctgtcgca agccgaccgt gagcagggac tggcgctgtt ctgctgcgcc
     1201 tcgccacgct ccgacctggt catcgaatcg cgggaagtgc gcaaggccgg tgacattccg
     1261 gtcaagacgc tgccggtgcg ggtgcagtcg ctcgaacgtg ccgctcccga cgtgatgatc
     1321 gtcaagctga agttgcccgc tagcgaacgc ctgcagtttc tcgccggaca gtacgtcgac
     1381 atcctgctca aggacggccg ccggcgcagc ttttcgcttg ccaacgcgcc gcacgacgac
     1441 gcgctgctcg aactgcacat ccgccacgtt cccggcggcc tgttcacgga acaggtattc
     1501 acgcagatga aggagaagga catcctgcgc atcaacggcc cgcacggcac cttcttcctg
     1561 cgcgaggact cgccgaagcc gatcgtgatg atcgccgggg gtacgggttt cgcaccgatc
     1621 aagtccgtcg tcgagcacgc cgtcgccgaa agctgcgagc gtccgatcac cgtctattgg
     1681 ggcgggcggc agcgcatcga cctctatgca ctggacgtgc cggagcgctg ggcaagccag
     1741 tcgaacattc gcttcgtacc ggtactctcg gaacccccgg cggaagactc gtggaccgga
     1801 cgcaggggtt tcgtccacgc ggcggcgatg gccgactttc ccgatctttc aggtcatcag
     1861 gtttatgcct gcggctcgcc ggcgatgatc gccgccgcaa agcgcgatct cgtcgacagg
     1921 tgtaagctac cggaggacga atttttcgcc gattcgttcg atttcgcgaa cgacaagcac
     1981 caggaacccg catgagcctc ttcatgaccc gtcagcccct gatcaatcgt cagggcaaga
     2041 tcatcgccac ccggctacgc ctgaatgcga ccgcagggga aagctcggcc accgtcgtgg
     2101 acgccctcaa cggactgaac gactgctggc cgcagagcga ccgcagcatc ttcgtcaatt
     2161 tcgacggcgt cgccggcgac atgggttttc tcgactggat cgcaccggcc aacgtcacct
     2221 tcgaactgaa cggcgccgcc tttgccggcg acgacgctgc cgcgttcgcg caggcactca
     2281 aggatgccga cctgtcgctg tgctacgact tcgacggcca tgccgcgcag gcattcggca
     2341 gcggcaccaa gttccgcttc gtcgggttcg atgccacccg tttttccgcc gcacagttga
     2401 aggcgctgct cgcgaagacg cagcccttcg gcatcccggt cgccttcaat gtcgacggcc
     2461 agcaggaatt caaggcctgc atggacgccg gcatgagtgc cgctgcaggc tggttcgtca
     2521 agcagcctgc gagcgctccg gccaagcagc tgaacgccag ccaggcacac atcgtcaggg
     2581 tcctcaatct ggtacgcaag aacgcggaag tgggccagat cgaatcggcg ctgaagcagg
     2641 atgtcgcgct ctcctacaag ctgctgcgct acatcaactc cgccggcttc gggctttcct
     2701 gcgaagtgca gtcgttcaag cacgccgtca ccatcctcgg ctacgacaag ctgaacaagt
     2761 ggctgtcgct gctgctcgtc accgccagca aggatccgat ggcccctacg ctgatgcaca
     2821 cggcgctgac gcgggcgcgc ctgatggagc agctcgggca cggactggtc gacaaatccg
     2881 agtacgacaa cctcttcatc accggcgcct tctcgatgct cgatctgctg ctcggcgtca
     2941 cgatggaaca ggcgctcgac gcgatgcacc tgccggaacc gatcaccgat gcgctgctcg
     3001 gcaatgccgg catctacgcg cccttcctgg cactggcgct ggcctgcgag ggcggggagg
     3061 tcgcatcgct ggccgagcag gccggcatgc tcggcctgac gggcgaacag ctcaatcgcg
     3121 cacaactcgc ggcgctgtcg ttcgccgaca cgatggaatc ctgacggcgg ctgtgcgccg
     3181 ctgcaaaaaa gaaggcgccc gcgggcgcct ttcttctttc ggacggctgg ctgccgttca
     3241 ctcgccgaga taggcttcgc gcaccttcgg gtcgtgcagc agctttgccg cttcgtcggt
     3301 cagcgtgatc tcgccgcttt ccatgacgta gccacgctga ctggtttcga gcgcgagctt
     3361 ggcgttctgc tcgatgagca ggatcgtcat gccttccgcc gcaacggtac ggacgacctc
     3421 gaagatcttc tgcaccatga tcggcgccag ccccatcgaa ggttcatcga gcagcagcag
     3481 cttcggccgg cccatcagcg cccgaccgat cgccagcatc tgctgctcgc caccggacag
     3541 ggtgcctgcc agctgcgcct cgcgctcctt gagacgggga aacaggccat acacatggtc
     3601 cgtatcgcgg gcgatctcgg ccttgtcgtc acgacagaac gcgcccatcg ccagattctc
     3661 ggcaatggtg aggcgcggga aaacgccgcg cccttccggc accagcgcaa tgccctcgcg
     3721 caccaggtgg tgcgaaggca aacgcgagat ttccttgccc tgatagcgga cgctgccgcc
     3781 ggcggcatcg agcatgcgcg ccagcgcctt cagcgtgctg gtcttgccgg caccgttggc
     3841 gccgatcaac gccaccgttt cgccgggatt gatgtggaag gtgatgccgc gcaccgcctg
     3901 gatgcctccg tagctgacac gcaggccctc gacgatcagc agcggttcga ttccgttgct
     3961 catgctgcca ctccgatata agcctcgatc acgcgcggat cgtgctgcac caccgacggc
     4021 acgtcctcga tcaccagcgc gccgtaatcg agcacggcga cacggtcaca gagccccatc
     4081 accagcttca cgtcatgctc gatcaacagg atggtcacgc catcggcgcg gatgccttcg
     4141 atcagcaggc gc
//
LOCUS       DAOQIE010000325         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_12936_length_4142_cov_7.959873, whole genome shotgun sequence.
ACCESSION   DAOQIE010000325 DAOQIE010000000
VERSION     DAOQIE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12936_length_4142_cov_7.959873"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..374
                     /locus_tag="PK144_16015"
     CDS             147..374
                     /locus_tag="PK144_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNF67281.1"
                     /translation="MRTPTRFLIAGLLSVSLAACAGMNHQDRSTVAGAAIGGVAGSVL
                     TNGDALGTVGGAAVGGVIGHEVSKPERRYRY"
     gene            complement(481..1182)
                     /locus_tag="PK144_16020"
     CDS             complement(481..1182)
                     /locus_tag="PK144_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF67282.1"
                     /translation="MLSFENVHTCYGKVEALKGVSLEVRQGEIVTLIGANGAGKTTLL
                     MTLCGSPRAASGCVRFEGRDITREPTHRIMQGGIAISPEGRRIFPGLTVMENLQMGGF
                     FADAAHIAQDVERVFTLFPRLKERQHQRAGTMSGGEQQMLAIGRALMSRPRLLLLDEP
                     SLGLAPLIIAQIFQIIQEIRDEGVTVFLVEQNAHRALALADRGYVLENGRIVLADSGP
                     ALLANDEIRKAYLGG"
     gene            complement(1194..1997)
                     /gene="livG"
                     /locus_tag="PK144_16025"
     CDS             complement(1194..1997)
                     /gene="livG"
                     /locus_tag="PK144_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HNF67283.1"
                     /translation="MSAGAASAASPARSAPLLTVEGLQMRFGGLLAVDGIGFEVGRGE
                     IFAIIGPNGAGKTTVFNCVSGFYRPSGGSIRLDGSEIAGRPGHRIARAGVVRTFQNIR
                     LFRKMTAVENLLVAQHNQLDTSWLSGLLALPRFRRAEREGLARAAFWLEQVGLRAHAN
                     FEAGNLSYGQQRRLEIARCMITRPRLLLLDEPAAGLNPQETRELDQLIRRLRDEHEVS
                     VLLIEHDMSLVMEISDRILVMEYGRPIGAGTPAEVRANPRVIKAYLGEE"
     gene            complement(1994..3280)
                     /gene="livM"
                     /locus_tag="PK144_16030"
     CDS             complement(1994..3280)
                     /gene="livM"
                     /locus_tag="PK144_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNF67284.1"
                     /translation="MAVQEPSPRTAATTTTTTTADSTLPLKDAGLAAVLTGVLTLPLL
                     GNHLIIDGTKTTLVTNYVPVLIAMALVFVVRLLRGPLDRALAAIPRPRLPAALGERQG
                     LWLAALVLAALVFPFVAARGAIDVLTFALTYVLLGLGLNIVVGFAGLLDLGYVAFYAV
                     GAYTYALLSQYFGLSFWLCLPLGGLLAALFGVLLGFPVLRLRGDYLAIVTLGFGEIIR
                     LVLNNWDSLTHGPDGINGIDRPTLFGLTFTKRAPEGHTTFFDFLGIPFDSQYAVIFLY
                     LIALALVLFTLFVINRLIRMPVGRAWEALREDEIACRSLGLNPTLVKLSAFALGAFFA
                     GLAGCFYAARLGAITPESFTFIESAVVLAIVVLGGMGSQLGVILAALALTLLPELARE
                     FSEYRMLVFGLVMVLMMIWRPQGLLPASRPRLELPQ"
     gene            complement(3283..>4142)
                     /gene="livH"
                     /locus_tag="PK144_16035"
     CDS             complement(3283..>4142)
                     /gene="livH"
                     /locus_tag="PK144_16035"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HNF67285.1"
                     /translation="IAIGYTMVYGIIGMINFAHGEIYMIGAYVGVVTLTALGAASGTL
                     PLTLVLAGALVAAIVVTAAYGWTVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQLG
                     QGARDVSVPPLLPGNLFSVEIGGFPVSLSVTRALIIGVTVVLMIALTLYIGRSRMGRA
                     CRACSEDMGMARLLGIDTDRVISFTFVLGAALAAVGGVLIGLSIGKINPFIGFVVGVK
                     AFTAAVLGGIGSIPGAMLGGVLLGLTETFASAFLPTEYKDVVAFSLLVLVLLFRPTGL
                     LGKPDIEKV"
BASE COUNT          717 a         1420 c         1449 g          556 t
ORIGIN      
        1 cgacgcatgg ccagtgtgtc ggtgccggcc atgcaaccat cgccgcactc cccgcccctg
       61 cggcccgctg cggaacctct gcgcatgaag cccggtcagt cgaaccatgg ggccgtcata
      121 aaaccacacc cgggagttcg aaaatcatgc gcacaccgac tcgtttcctg atcgccggcc
      181 tgctctccgt cagcctcgcc gcctgtgccg gcatgaatca tcaggaccgc agcaccgtgg
      241 ccggtgccgc catcggcggc gtggccggtt cggtgctgac gaatggcgac gcgctcggca
      301 cggtcggcgg tgccgccgtc ggcggtgtca tcggccacga ggtcagcaag cccgagcggc
      361 gatatcgcta ctgaactgcc tttcgcaggg accgggagcg gaggctcccg gactgcggcg
      421 ggcagcaggc cggtgcagta aagagcaggc agggactgcc cttccctcaa gcagcccgga
      481 tcacccgccc agatacgcct tcctgatctc gtcgttcgcg agcagcgccg gcccggagtc
      541 ggcaagcacg atgcggccgt tttcgagcac gtagccgcga tcggcgagtg cgagtgcgcg
      601 gtgggcgttc tgttcgacca ggaacacggt cacgccctcg tcgcggatct cctgaatgat
      661 ctggaagatc tgcgcgatga tcagcggcgc cagcccgagc gagggttcgt cgagcagcag
      721 caggcgcggg cggctcatca gcgcgcggcc gatcgcgagc atctgctgct cgccgcccga
      781 catcgtgccg gcgcgctggt gctggcgctc cttcaggcgc gggaacagcg tgaacacgcg
      841 ctcgacatcc tgcgcgatgt gcgcggcatc ggcgaagaag ccgcccatct gcaggttttc
      901 catgaccgtc aggcccggga agatgcgccg gccctcgggc gagatggcga tgccgccctg
      961 catgatgcgg tgcgtcggct cgcgcgtgat gtcgcggccc tcgaagcgca cgcagccgct
     1021 tgcggcacgc gggctgccgc agagcgtcat cagcagcgtg gtcttgccgg cgccgttggc
     1081 gccgatcagc gtgacgatct cgccctggcg gacttcgagc gacacgccct tgagcgcctc
     1141 gaccttgccg tagcaggtgt ggacgttttc gaagctcagc atcggcgcgc tcatcattcc
     1201 tccccgagat aggccttgat gacgcgcgga ttggcccgca cctcggccgg cgtgccggcg
     1261 ccgatcgggc ggccgtactc catgaccagg atgcggtcgg agatttccat caccagactc
     1321 atgtcgtgct cgatcagcag caccgagacc tcgtgctcgt cgcgcagccg gcggatgagc
     1381 tggtcgagtt cgcgggtttc ctgcgggttg aggccggcgg cgggttcgtc gagcagcagc
     1441 aggcgcgggc gcgtgatcat gcagcgcgcg atctccagcc ggcgctgctg gccgtagctc
     1501 aggttgccgg cctcgaagtt cgcgtgcgcg cgcaggccga cctgctccag ccagaaggcg
     1561 gcacgcgcga ggccctcgcg ttcggcgcgc cggaagcgcg gcagcgccag caggccgctg
     1621 agccagctgg tgtcgagctg gttgtgctgc gccaccagca ggttctcgac cgcggtcatc
     1681 ttccggaaca ggcggatgtt ctggaaggtg cgcacgacgc cggcgcgggc gatgcgatgc
     1741 ccgggccggc cggcgatctc gctgccgtcg agccggatgc tgccgccgct cgggcggtag
     1801 aagccgctga cgcagttgaa gacggtggtc ttgccggcgc cgttggggcc gatgatcgcg
     1861 aagatctcgc cgcgcccgac ctcgaagccg atgccgtcga cggcgagcag gccgccgaag
     1921 cgcatctgca gaccctcgac cgtcagcagc ggcgcggagc gcgcgggcga tgcagcagaa
     1981 gcagccccgg cgctcattgc ggcagctcca gacgcgggcg cgaggccggc agcaggccct
     2041 gcggacgcca gatcatcatc agcaccatga ccagcccgaa caccagcatc cggtactccg
     2101 agaactcgcg ggccagctcc ggcagcagcg tcagcgccag cgcggcgagg atcacgccga
     2161 gctgcgagcc catgccgccg agcacgacga tcgcgagcac gaccgccgat tcgatgaagg
     2221 tgaacgattc cggcgtgatc gcgccgagcc gcgcggcgta gaagcagccg gccagccccg
     2281 cgaagaacgc cccgagcgcg aaggccgaga gcttcaccag cgtcggattc aggccgagcg
     2341 agcggcaggc gatctcgtcc tcgcgcagcg cctcccaggc gcgcccgacc ggcatgcgga
     2401 tcaggcggtt gatcacgaac agcgtgaaca gcaccagcgc gagcgcgatc aggtacagga
     2461 agatcaccgc gtactgcgaa tcgaacggga tgccgaggaa gtcgaagaag gtcgtgtgac
     2521 cttcgggtgc gcgcttcgtg aaggtcaggc cgaacagcgt cgggcggtcg atgccgttga
     2581 tgccgtccgg gccgtgcgtg aggctgtccc agttgttcag caccaggcgg atgatctcgc
     2641 cgaagccgag cgtgacgatg gcgaggtaat ccccgcgcag gcgcagcacc gggaagccga
     2701 gcagcacgcc gaagagcgcg gcgagcaggc cgccgagcgg caggcagagc cagaacgaca
     2761 gcccgaagta ctgcgacagc agtgcgtagg tgtaggcccc gaccgcgtag aaggcgacgt
     2821 agccgagatc gagcaggccg gcgaagccga cgacgatgtt caggcccagc ccgagcagca
     2881 cgtaggtcag cgcgaaggtc agcacgtcga tggcgccgcg cgcggcgacg aacgggaaca
     2941 ccagcgccgc cagcaccagc gccgcgagcc acagcccctg acgctcgccg agcgcggccg
     3001 gcagccgcgg gcgcggaatc gccgccagcg cgcgatcgag cgggccgcgc agcaggcgca
     3061 cgacgaacac cagcgccatc gcgatcagca ccggcacgta gttcgtgacc agcgtcgtct
     3121 tcgtgccgtc gatgatcaga tggttgccga gcagcggcag cgtcagcacg ccggtcagca
     3181 cggccgcgag cccggcatcc ttcagcggca gcgtgctgtc cgccgtcgtc gtcgtcgtcg
     3241 tcgtcgctgc cgtacggggg gaaggttctt gcacagccat cgtcacacct tctcgatgtc
     3301 gggcttgccg agcaggccgg tcggtcggaa cagcagcacc agcaccagca ggctgaaggc
     3361 cacgacatcc ttgtattcgg tcggcaggaa ggccgaggcg aaggtttcgg tcagtccgag
     3421 cagcacgccg ccgagcatcg cgcccggaat gctgccgatg ccgccgagca cggcggccgt
     3481 gaacgccttg acgccgacca cgaagccgat gaacgggttg atcttgccga tcgacaggcc
     3541 gatcagcacg ccgccgaccg ccgcgagcgc ggccccgagc acgaaggtga aggagatcac
     3601 ccggtcggtg tcgatgccga gcagccgggc catgcccatg tcctcggagc aggcgcggca
     3661 ggcacgcccc atgcgcgagc ggccgatata cagcgtcagc gcgatcatca gcacgacggt
     3721 cacgccgatg atcagcgcgc gcgtcaccga caggctgacc ggaaagccgc cgatctcgac
     3781 cgagaacagg ttgccgggca gcagcggcgg caccgagaca tcgcgcgcgc cctggccgag
     3841 ctgcacgtag ttctgcagga agatcgacat gccgatcgcc gagatcagcg gcaccagccg
     3901 cggaccgccg cgcagcggcc ggtaggcgac gcgctcgacc gtccagccgt aggcagcggt
     3961 gacgacgatc gccgccacca gcgccccggc gagcaccagc gtcagcggca gcgtgccgct
     4021 cgcggcgccg agcgcggtca gcgtcacgac gccgacgtag gcgccgatca tgtagatctc
     4081 gccgtgcgcg aaattgatca tgccgatgat gccgtacacc atcgtgtagc cgatcgcgat
     4141 ca
//
LOCUS       DAOQIE010000326         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13136_length_4101_cov_6.462679, whole genome shotgun sequence.
ACCESSION   DAOQIE010000326 DAOQIE010000000
VERSION     DAOQIE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13136_length_4101_cov_6.462679"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK144_16040"
     CDS             complement(<1..773)
                     /locus_tag="PK144_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005890620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF67286.1"
                     /translation="MPIPIRSKQDWQALDAAHHLHPFTDYADLAAKGARIISWAEGAH
                     LHDADGRTILDGMSGLWCVNLGYGRRELAEAAQRQMLELPYYNNFFQTAHPPAIELAH
                     RLAEFAAPDYTRVFFTGSGSESVDTAIRMIWRYWQLQGQPQRNVIIGRRNGYHGSTIA
                     GASLGGMKAVHAQLAHPLPGFVHIDQPYAFGEGAGLDPAAFGLERARQLAQKIEEIGP
                     DKVAAFFAEPIQGAGGVIIPPDTYWPEIERICRHHGILLV"
     gene            complement(906..2408)
                     /locus_tag="PK144_16045"
     CDS             complement(906..2408)
                     /locus_tag="PK144_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNF67287.1"
                     /translation="MSTPPTLADWHARAAALQPLKQLLIDGRWQDAADGATLPCVNPA
                     SGQTWTSVAAGSSADIDRAVRAARAAFEDRRWAGQAPAARKRVLQRWAELVEAHRDEL
                     ALLETLNVGKPIRDALAIDVPATARCLRWYGEAIDKLYDEIAPTAEQSLALIRREPVG
                     VVGVVVPWNFPLIMSAWKLGPALAAGNSVVLKPAEQSPLSALKLAELALEAGLPPGVL
                     NVVTGYGHSAGRALGLHEDVDAVGFTGSTEVGKLFLQYAGQSNLKRVWLECGGKTPHI
                     ILADCPDLDAAATAAAWGIFFNQGEMCTAGSRLLVDRRIKDQVLERIVAVGRTLAPGD
                     PLDPATQLGAIVSKEQWQRVLDYIDIGRAQGATLALGGAATRGATGGFYIEPTVFDGV
                     NNAMRIAQEEIFGPVLSVLAVDGAEQAVRIANDTMYGLAAAVWTRDLATAHRVSRQLR
                     AGLVWVNCFDADDITTPFGGYRQSGNGRDKSLHAFDKYTELKTIWMRIGD"
     assembly_gap    2550..2649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2650..3516)
                     /locus_tag="PK144_16050"
     CDS             complement(<2650..3516)
                     /locus_tag="PK144_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNF67288.1"
                     /translation="MNDRHEPAGYPDPDAALPPAPDDPERLLLHADLDGELDPAASLA
                     LARRRAADPAFAAEAERIAALHRALSRISTEHGPSAALRARLAQPWAQAAADVPVTSP
                     VTAPVTPLPAAQTRTRRRGWHGDWRALAASCLLSAGLVYGSAALYFAPDAQQAQIAAT
                     VAGHRRALLAQHPVDVASSDRHTVKPWLGSRLALAPTVIDLAAGGYPLLGARIEIVDG
                     RPAPTLVYGHREHLISLSLIPVGSREESSPELSTVDGYGVLRWQHAGMAHVAVSDIDR
                     PGLDDFVAQYRAA"
     gene            complement(3513..4094)
                     /locus_tag="PK144_16055"
     CDS             complement(3513..4094)
                     /locus_tag="PK144_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF67289.1"
                     /translation="METVRTVTTDIADRTRAQQRYAEVVMPWIDGGYALARWLTGNAT
                     DAEDVLQDACARAWAALGSYAGGSARAWFLTIVRNCAYTWLARNRPHALEYTDDPAAA
                     EAERGIELPGYAPQPPVPPEALAIEQADRERLAAAVAALPPVFRETLVLREFHALGYR
                     EIAELTGVPVGTVMSRLARARSRLIADLGGSPA"
BASE COUNT          533 a         1341 c         1552 g          575 t
ORIGIN      
        1 accagcagga tgccgtggtg ccggcagatg cgttcgatct ccggccagta ggtgtcgggc
       61 gggatgatga cgccgccggc gccctggatc ggttcggcga agaaggccgc gaccttgtcc
      121 gggccgattt cctcgatctt ctgcgcgagc tggcgggcac gctccagccc gaatgcggcc
      181 gggtccagcc ctgcgccctc gccgaaggcg tagggctggt cgatgtgcac gaagcccggc
      241 agcgggtgcg cgagctgcgc gtgcacggcc ttcatgccgc cgaggctggc gccggcgatg
      301 gtcgagccgt gatagccgtt gcgccggccg atgatgacgt tgcgctgcgg ctgcccctgc
      361 agctgccagt agcgccagat catgcggatc gcggtatcga ccgactcgga gccggagccg
      421 gtgaagaaca cgcgcgtgta atcgggcgcc gcgaactcgg cgagccggtg cgcgagctcg
      481 atcgccggcg ggtgggcggt ctggaagaag ttgttgtagt acggcagctc cagcatctgg
      541 cgctgcgcgg cctcggcgag ctcgcgccgg ccgtagccga ggttcacgca ccagagcccg
      601 gacatgccgt cgaggatggt gcggccatcg gcgtcatgca gatgggcgcc ttcggcccag
      661 ctgatgatgc gcgcgccttt cgcggccagg tcggcgtaat cggtgaacgg gtgcaggtga
      721 tgggcggcgt cgagggcctg ccagtcctgc ttgctgcgga tcgggatggg catcttgccg
      781 ggaccttcgt gagtgcgagg gaatgcttgg gtgcgaggga atgcggaggg acagggcggg
      841 cgatgggctc cgtggctgac cggcggacgt gccgcattcc gtccgcgccg gcggcgtgca
      901 ggcgctcagt cgccgatgcg catccagatt gtcttcagct ccgtgtactt gtcgaaggcg
      961 tgcagggact tgtcgcggcc gttgccggac tggcggtagc cgccgaaggg cgtggtgatg
     1021 tcatcggcat cgaagcagtt gacccagacc agcccggcac gcagctgccg cgaaacccgg
     1081 tgcgcggtgg cgagatcgcg cgtccagacc gcggcggcaa gcccgtacat ggtgtcgttg
     1141 gcgatgcgca cggcctgttc ggcgccgtcg acggcgagca ccgacagcac cgggccgaag
     1201 atttcctcct gcgcgatgcg catggcgttg ttgacgccgt cgaacacggt cggctcgatg
     1261 tagaagccgc cggtcgcacc gcgcgtcgcc gcaccgccga gcgccagcgt cgcgccctgc
     1321 gcgcggccga tgtcgatgta atcgagcacg cgctgccatt gttccttgct gacgatggcg
     1381 ccgagctgcg tggccggatc gagcggatcg ccgggggcga gcgtgcgccc gaccgcgacg
     1441 atgcgctcca gcacctggtc cttgatgcgg cgatcgacga gcagccgcga gccggccgtg
     1501 cacatctcgc cctggttgaa gaagatgccc caggcggcgg cggtcgcggc ggcgtcgagg
     1561 tccgggcagt cggcgaggat gatgtgcggg gtcttgccgc cgcattcgag ccagacgcgc
     1621 ttgaggttcg actggccggc gtactgcagg aacagcttgc cgacctcggt cgagccggtg
     1681 aagccgacgg cgtcgacatc ctcgtgcagg ccgagcgcgc gcccggcgct atggccgtag
     1741 ccggtgacga cgttcagcac gcccggcggc agcccggctt cgagcgcgag ttcagcgagc
     1801 ttcagcgccg acagcggcga ctgctcggcg ggcttgagca ccaccgagtt gccggccgcg
     1861 agcgccgggc cgagcttcca ggcgctcatg atcagcggga agttccacgg cacgaccacg
     1921 ccgaccacgc cgaccggctc gcgccggatc agcgccagcg actgctcggc ggtcggggcg
     1981 atctcgtcgt agagcttgtc gatcgcctcg ccgtaccagc gcaggcagcg cgcggtggcc
     2041 ggcacgtcga tggcgagcgc gtcgcggatc ggcttgccga cgttcagtgt ttcgagcagc
     2101 gccagttcgt cgcgatgcgc ctcgaccagc tcggcccagc gctggagcac gcgcttgcgc
     2161 gctgccggtg cctgtccggc ccagcgccgg tcctcgaagg ccgcgcgcgc cgcgcgcacg
     2221 gcgcggtcga tgtcggcgct gctgccggcg gcgaccgagg tccacgtctg gccgctggcc
     2281 gggttcacgc agggcagcgt cgcgccgtcg gcggcgtcct gccagcggcc gtcgatcagc
     2341 agctgcttca gcggttgcag cgcggcggca cgggcgtgcc agtcggcgag ggtcggaggg
     2401 gtggacatga cgactccagc gggcttgcgg gggcgggacg acgggccgga aaatgtgatc
     2461 acatcctaga agccagcggc aagcccctgt cgccagtgcc gcttaccgga gattgcgccg
     2521 ttggccaccc cgaatcccgc cccgaatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnc gccgcccggt actgcgcgac gaaatcatcg agccccgggc ggtcgatgtc
     2701 cgataccgcg acgtgcgcca tgccggcgtg ctgccagcgc agcacgccat agccatcgac
     2761 cgtcgacagc tccggcgagc tttcctcgcg cgagccgacc gggatcaggc tcaggctgat
     2821 caggtgttcg cggtggccgt agaccagcgt cggcgccggg cggccgtcga cgatctcgat
     2881 gcgcgcaccg agcagcggat agccgccggc ggcgaggtcg atgacggtcg gggccagcgc
     2941 cagccggctg ccgagccagg gcttgaccgt gtgccggtcg ctgctcgcga catcgaccgg
     3001 gtgctgcgcg agcagcgcgc ggcggtggcc ggcgacggtg gccgcgatct gcgcctgctg
     3061 tgcatcgggc gcgaagtaca gcgcggcgct gccgtacacg aggccggcgc tcagcaggca
     3121 gctcgcggcc agcgcccgcc agtcgccgtg ccagccgcgg cggcgggttc gggtctgggc
     3181 ggcagggagc ggggtgacgg gcgcggtcac ggggctggtc acgggcacat cggcggcggc
     3241 ctgcgcccac ggctgcgcga gccgggcacg cagcgccgcg gacgggccat gttcggtcga
     3301 gatgcgcgac aaggcccggt gcagcgcggc gatccgttcg gcctcggcgg cgaatgccgg
     3361 gtcggcggcg cggcggcggg cgagggcgag gctcgccgcc gggtcgagtt cgccgtcgag
     3421 atcggcgtgc agcagcaggc gctccggatc gtccggggcc ggcggcagcg cggcgtcggg
     3481 atcggggtag ccggcgggtt cgtggcggtc gttcatgccg ggctcccgcc gaggtcggcg
     3541 atcaggcggc tgcgggcgcg ggcgaggcgc gacatgaccg tgccgaccgg cacgccggtc
     3601 agttcggcga tctcgcggta gccgagcgcg tggaattcgc gcagcaccag ggtttcgcgg
     3661 aacaccggcg gcagcgccgc gaccgcggcg gcgaggcgct cgcggtcggc ctgctcgatc
     3721 gccagcgcct cgggcggtac ggggggctgc ggcgcgtagc cgggcagctc gatgccgcgc
     3781 tcggcttcgg cagcggccgg atcgtcggtg tattcgagcg cgtgcgggcg gttgcgcgcg
     3841 agccaggtgt acgcacagtt gcgcacgatc gtgaggaacc aggcacgcgc gctgccgccg
     3901 gcgtagctgc cgagcgcggc ccaggcgcgg gcgcaggcgt cctgcagcac gtcctcggcg
     3961 tcggtggcgt tgccggtcag ccagcgcgcc agcgcatagc caccatcgat ccagggcatg
     4021 accacctccg cgtagcgctg ctgcgcccgg gttctgtcag cgatgtcggt cgtcacggtg
     4081 cgaaccgtct ccataagccg c
//
LOCUS       DAOQIE010000327         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13154_length_4098_cov_6.675241, whole genome shotgun sequence.
ACCESSION   DAOQIE010000327 DAOQIE010000000
VERSION     DAOQIE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13154_length_4098_cov_6.675241"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK144_16060"
     CDS             complement(<1..508)
                     /locus_tag="PK144_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNF67290.1"
                     /translation="MEEQVGQLWHRLVTRAASRRHPEAAVRLAEVQKTVAVLFRALGG
                     DGALSVAGAQALEHGARRGWLERIAGSGREAALAWRDETTLRLPECIDLFADPALNRD
                     LYLWLAALAAQAGTEDDSDADWLVASARQTCAALARFPGLAPRYEQLVAAHLAQRPDP
                     GRLPADEAA"
     gene            complement(690..1457)
                     /locus_tag="PK144_16065"
     CDS             complement(690..1457)
                     /locus_tag="PK144_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF67291.1"
                     /translation="MKPESAVRIVQISDCHLRSTPGAQSWGVDVDAGLRAVLTLATGL
                     QPALLLATGDLVHDEGTPAYERLRDDFLLPSGLPVAALPGNHDDSDALAACWSSGPVQ
                     RRRQLETGGWQLLLLDTRVPQSPAGRLAPSELDFLQQSLAARPQTPTLVALHHPPLLT
                     GCAGFDAIALDNRAELFGVLKQAPQVRAVVFGHLHAEVDTVIDGIRCLGCPSTCIQFD
                     LQAPEPAADAAHGPGCRLLELFPDGRLQTQVLRVEGA"
     gene            1535..2191
                     /locus_tag="PK144_16070"
     CDS             1535..2191
                     /locus_tag="PK144_16070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF67292.1"
                     /translation="MKAIQLTETLYDYLIDVSLRETDVQRRLRAYTSTLERARMQIAP
                     EQGQFMQWLVRLTGARRIVEVGTFTGYSALAMALALPPDGRLIACDIDPVHTATAQQY
                     WQEAGVADRIELRLGPALDTLDGLITEFGRNKIDLAFIDADKTAHLFYYERLLEMLRP
                     GGVILIDNTLWGGSVADPAAHDADTLAIRGLNLLLRDDDRIDLAHLPVGDGLALALKR
                     "
     gene            2220..2867
                     /locus_tag="PK144_16075"
     CDS             2220..2867
                     /locus_tag="PK144_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNF67293.1"
                     /translation="MSRRVRRLLTVVLVLVLTPALLMAWFMARVAIYADNRDEGQADA
                     AVVLGAAAWFTRPSPVFEERIKHALDLYKEGRVRAIVFTGGVGRGDTRSEAEVARNYA
                     LRRGVPESAIHVETASRSTFGNLVNARLLLERNGLRRVLLVSDPLHMRRAMTMARDLG
                     IDARPSPTPTTRFQSFSTQFDLLLKETFDYARYKLGRHFLDFDEPTAGYAAAGES"
     gene            complement(2913..3290)
                     /locus_tag="PK144_16080"
     CDS             complement(2913..3290)
                     /locus_tag="PK144_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF67294.1"
                     /translation="MNEQVQFIEVDGQPAFAVVPVALWRRLAARAEDIEDADAIAQAK
                     AADDGARIPGAVAFAIAGGTHPVRAWREHHAMTQAALAQAAGISKGYLSQVEAGRRTG
                     TVDTLRALAQALGVTLADLDGSD"
     gene            complement(3294..3557)
                     /locus_tag="PK144_16085"
     CDS             complement(3294..3557)
                     /locus_tag="PK144_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNF67295.1"
                     /translation="MYRIEFTRPALKDLRALPASLVATLRTKLEAVAAEPYGAHPNVK
                     RLSGSTGYRLRVGDWRVLYELEDDRLVVLVLRVAPRGQAYKRR"
     gene            complement(3741..4067)
                     /locus_tag="PK144_16090"
     CDS             complement(3741..4067)
                     /locus_tag="PK144_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67296.1"
                     /translation="MSMEFRGTGNPRYLRVLALLLARPARREEIDRAAGALNGPDLMA
                     NLRALGLEAPCTRVDAIDRDGRECRPGVYRLTGGDRRRLAEWMRGRHVRLPPVTPDLF
                     GDGGAS"
BASE COUNT          599 a         1508 c         1416 g          575 t
ORIGIN      
        1 ccgcggcttc atcggccggc agccggccgg gatcggggcg ctgcgcgagg tgcgcggcca
       61 cgagttgctc gtagcgcggc gcgaggccgg ggaagcgcgc gagggcggcg caggtctgcc
      121 gggcgctcgc gaccagccag tcggcatcag agtcgtcctc tgtgcccgcc tgcgcggcga
      181 gcgcggccag ccacaggtac aggtcgcgat tcagcgccgg gtctgcaaac aggtcgatgc
      241 attcgggcag ccgcagcgtc gtctcgtcgc gccacgccag cgccgcctcc cgcccgctgc
      301 cggcgatgcg ctccaaccag ccgcgccgcg ccccgtgctc cagcgcctgc gccccggcca
      361 cgctcagcgc cccgtcaccg ccgagcgccc ggaacagcac ggcaaccgtc ttctgcacct
      421 cagccagccg caccgcggct tcggggtgcc gccggctggc cgcgcgcgtg accagccggt
      481 gccagagctg accgacctgt tcttccatct gcggaggact ccggaggcgc gccgcgggcg
      541 caggcaaagg cggattatcc ggccgcggac ggtgcagatg gcggcggcta ccacctgtgc
      601 gccgcgacac agccggcagg cgcaggcaga ggcagcgctg catcgccgcc ggacgggctt
      661 tcgcaccggc tctcgcgcga gccgcgcggc tacgcgccct cgacccgcag cacctgcgtc
      721 tgcagccgcc cgtccgggaa cagttccagc agccggcagc ccggaccgtg cgcggcatcg
      781 gccgccggtt ccggcgcctg caggtcgaac tggatgcagg tcgacgggca gccgaggcag
      841 cggatgccgt cgatgacggt atcgacctcg gcgtgcaggt ggccgaacac cacagcccgc
      901 acctgcggcg cctgcttcag caccccgaac agttcggcgc ggttgtcgag ggcgatcgcg
      961 tcgaaaccgg cgcagccggt cagcagcggc gggtggtgca gcgcgaccag cgtcggggtc
     1021 tgcggccgcg cggcgaggct ttgctgcagg aagtcgagtt cggacggcgc gaggcgccca
     1081 gccgggctct gcggcacgcg cgtatcgagc agcagcagtt gccagccgcc ggtttcgagc
     1141 tgccggcgcc gctgcaccgg acctgaactc cagcaggcgg cgagggcgtc ggagtcgtcg
     1201 tggttgccgg gcagagccgc gaccggcagg ccgctcggca gcaggaagtc atcgcgcagg
     1261 cgttcgtagg ccggtgtgcc ttcgtcgtgg accagatcgc cggtcgcgag cagcagcgcc
     1321 ggttgcagcc cggtcgcgag ggtcagcacg gcgcgcaggc cggcatcgac atcgacaccc
     1381 cagctctgcg cgcccggcgt gctgcgcagg tgacagtcgc tgatctgcac gatgcgcacg
     1441 gcggattcgg gtttcatcgg caaggctccg gcaatcggcg ggatacgggt atatgactat
     1501 cattgtcccc ccgccgccgg agcgccgcgt ccccatgaaa gccatccagc tgaccgagac
     1561 cctgtacgac tacctgatcg acgtgtcgct gcgcgagacc gacgtgcagc ggcggctgcg
     1621 cgcgtacacc tcgacgctgg agcgcgcgcg catgcagatc gcgcccgaac agggccagtt
     1681 catgcagtgg ctggtacgcc tgaccggcgc gcggcgcatc gtcgaggtcg gcaccttcac
     1741 cggctacagc gcgctggcga tggcgctggc gctgccgccg gacggccggc tgatcgcctg
     1801 cgacatcgac ccggtgcata ccgcgaccgc gcagcagtac tggcaggagg ccggcgtcgc
     1861 cgatcgcatc gagctgcgcc tcggtccggc actcgacacg ctcgacgggc tgatcaccga
     1921 gttcggccgc aacaagatcg acctggcctt catcgatgcc gacaagaccg cgcacctctt
     1981 ctattacgag cgcctgctgg aaatgctgcg ccccggcggc gtgatcctga tcgacaacac
     2041 gctctggggc ggcagcgtcg ccgacccggc cgcgcacgac gccgacacgc tggcgatccg
     2101 tggcctgaac ctgctgctgc gcgatgacga ccgcatcgac ctggcgcacc tgccggtcgg
     2161 cgacggtctg gcgctggcgc tgaagcgctg accactggcc gtgaggaccg ataccgaaga
     2221 tgagccgccg cgtccgccgc ctgctgaccg tcgtgctggt gctggtgctg acgccggcgc
     2281 tgctgatggc ctggttcatg gcgcgcgtgg cgatctacgc cgacaaccgc gatgaaggcc
     2341 aggccgatgc ggcggtcgtg ctcggggccg ccgcctggtt cacgcggccg tcgccggtgt
     2401 tcgaggagcg catcaagcac gcgctcgatc tctacaagga aggccgcgtg cgcgcgatcg
     2461 tgttcaccgg cggcgtcggg cgcggcgaca cgcgctccga ggccgaagtc gcgcgcaact
     2521 acgcgctgcg ccgcggcgtg ccggagtcag ccatccacgt cgagaccgcc tcgcgctcga
     2581 ccttcggcaa cctcgtcaac gcccggctgc tgctggagcg caatggcctc aggcgcgtgc
     2641 tgctggtctc ggacccgctg cacatgcgcc gcgcgatgac gatggcccgc gacctcggca
     2701 tcgatgcccg cccgtcaccg acaccgacca cgcgcttcca gagcttctcg acgcagttcg
     2761 acctgctgct gaaggagacc ttcgactacg cccgctacaa gctcggccgc cacttcctgg
     2821 acttcgacga gccgaccgcc ggctacgcgg ccgcgggcga gtcgtgaccg gcacggccga
     2881 tgcgcgccgg caagggcatc gggcgccagg gttcagtcgc tgccgtcgag gtccgcaagc
     2941 gtcacgccga gcgcctgcgc cagtgcgcgc agcgtgtcca cggtcccggt gcgccggccg
     3001 gcctcgactt gcgacaggta gcccttcgag atgcccgccg cctgtgcgag ggcggcttgc
     3061 gtcatggcgt ggtgctcgcg ccacgcccgc accggatgcg taccgcccgc gatggcgaag
     3121 gcgacggcac ccggaatgcg cgcgccgtca tcggcggctt tcgcctgcgc gatggcgtcg
     3181 gcgtcctcga tgtcctcggc ccgtgcggct agcctgcgcc acagggcgac cgggacgacg
     3241 gcgaacgccg gctgcccgtc cacttcgatg aactgcacct gttcgttcat gcctcacctt
     3301 cgcttgtaag cctgtccgcg cggggccacg cgcagcacca gcaccaccag ccggtcatcc
     3361 tccagttcgt acagcacccg ccagtcaccg acccgcaacc ggtagccggt cgagccgctc
     3421 agtcgtttga cgttcgggtg tgcgccgtaa ggctcggccg cgaccgcttc gagtttcgtg
     3481 cgcaacgtcg ccaccagaga tgccggcagc gcgcgcaggt ctttcagcgc cgggcgcgtg
     3541 aactcgatgc ggtacatgca cgcatattag caatccgcta acggtcacgt cagcaaaacg
     3601 ctttgcccgc cccgcgccgc taccctgccc gttcctcgac gtgcgtgcgc cagtagtcag
     3661 cgcagcagca gccacggccc gaaaggcggg acgggcgccc gtgcgggcgc cctcctgcgg
     3721 gtggacttgc gcttgcgcgg tcatgaagca ccgccatcgc cgaacaggtc cggcgtcacc
     3781 ggcggcagac gcacatgccg gccgcgcatc cattcggcca gccggcgccg gtcaccaccg
     3841 gtcagccggt acacgcccgg tcggcattcg cgaccgtcgc ggtcgatggc gtcgacgcgc
     3901 gtgcagggtg cctccagccc gagtgcgcgg agattcgcca tcaggtccgg accattcaat
     3961 gcaccggcag cccggtcgat ttcctcacgc cgcgccggcc gtgccaatag cagtgccagc
     4021 acgcgcaggt aacgcggatt gccagtgccg cggaattcca tactcatacc gcaccgccca
     4081 gcagcagggc tgcgacga
//
LOCUS       DAOQIE010000328         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13206_length_4087_cov_9.116815, whole genome shotgun sequence.
ACCESSION   DAOQIE010000328 DAOQIE010000000
VERSION     DAOQIE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13206_length_4087_cov_9.116815"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="clpX"
                     /locus_tag="PK144_16095"
     CDS             <1..742
                     /gene="clpX"
                     /locus_tag="PK144_16095"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HNF67297.1"
                     /translation="GIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIASV
                     PPQGGRKHPQQEFLQVDTSNILFICGGAFAGLDKVIRSRSEKGGIGFSAEVKSPTPSR
                     NTGETLRDVEPEDLIKYGLIPEFVGRLPVIATLDELDEAALVRILTEPKNAITKQFAR
                     LFEMENCEIEFRDEALRAVARKAMERKTGARGLRTILEHVLLDTMYDLPSLDDVSRVV
                     VDDSVVRGDAKPFMIYENAGPRRAASSD"
     gene            883..3327
                     /gene="lon"
                     /locus_tag="PK144_16100"
     CDS             883..3327
                     /gene="lon"
                     /locus_tag="PK144_16100"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNF67298.1"
                     /translation="MEPRPLTDDSDPLPDSHPIPILPLRDVVVYPHMVIPLFVGREKS
                     IAALDAAMQTDKRILLVSQKSAEVDDPGVEDLYRIGTLSNILQLLKLPDGTDKVLVEG
                     GQRARITGFSDGHAYFAAGIEIIGADYEESRETEVLMRSLLGIFDQYVKLNKKIPGEV
                     LSSLAGIEDAGRLADTIAAHMALKLDEKQKILEIESVDARLEHLLHLVESEIDILQVE
                     KRIRTRVKQQMEKSQREYYLNEQMKAIQKELGDLEDAPNEADELAQKIEQAGMPKEAK
                     AKAVAELNKLKMMSPMSAEATVVRNYIDWLVSVPWKKRTKIRHDLAEAERILEADHYG
                     LETVKERILEYLAVQQRAKKLKGPILCLVGPPGVGKTSLGRSIARATGRKFVRMSLGG
                     VRDEAEIRGHRRTYIGSMPGKIVQNLSKIGTRNPLFLLDEIDKMSMDFRGDPSSALLE
                     VLDPEQNNTFGDHYLEVDCDLSDVMFVATANSLNIPGPLLDRMEVIRLPGYTEDEKTH
                     IALKYLVPKQVEANGLHADELEIREAAVRDIIRHYTREAGVRNLEREISKICRKAVKS
                     ILLKPREERARTAVTPKTLDKYLGVRRFRFGLAEDQDQIGHVTGLAWTEVGGELLTIE
                     AALMPGKGKLTYTGQLGEVMQESIQAAMTVVRSRSDALGIDPAFFEKQDLHIHVPEGA
                     TPKDGPSAGTAMCTALVSALTRIPVRANVAMTGEITLRGAVLPIGGLKEKLLAAHRGG
                     ITTVLIPEDNRKDLAEIPKNILAKIEVRPVQWIDQILAQALVHLPEPSKPDAELPVSQ
                     ETPARSPAASEPVRPH"
     gene            3489..3761
                     /locus_tag="PK144_16105"
     CDS             3489..3761
                     /locus_tag="PK144_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF67299.1"
                     /translation="MNKSELIDAVADATGITKSDAGRSVEAVLGAITSALQGGDQVTL
                     VGFGTFSVRERAARTGRDPRSGQAIEIKASKNPVFKAGKALKDALK"
     gene            3776..3851
                     /locus_tag="PK144_16110"
     tRNA            3776..3851
                     /locus_tag="PK144_16110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3809..3811,aa:Val,seq:tac)
     gene            3868..3944
                     /locus_tag="PK144_16115"
     tRNA            3868..3944
                     /locus_tag="PK144_16115"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3902..3904,aa:Asp,seq:gtc)
BASE COUNT          801 a         1293 c         1324 g          669 t
ORIGIN      
        1 cggcatcgtc tacatcgacg agatcgacaa gatctcgcgc aagtccgaca acccgtcgat
       61 cacgcgcgat gtctccggcg aaggcgtgca gcaggcgctg ctgaagctga tcgagggcac
      121 gatcgcctcg gtgccgccgc agggcgggcg caagcatccg cagcaggagt tcctgcaggt
      181 cgatacctcg aacatcctgt tcatctgcgg cggtgcgttt gccggtctcg acaaggtcat
      241 ccgcagccgc tccgagaagg gcggcatcgg cttctcggcc gaggtcaaga gtccgacccc
      301 gagccggaac accggtgaga ccctgcgcga cgtcgagccc gaggatctga tcaagtacgg
      361 cctgatcccc gagttcgtcg gccgtctgcc ggtgatcgcg acgctcgacg agctcgacga
      421 ggcggcgctg gtgcgcatcc tgaccgagcc gaagaacgcc atcaccaagc agttcgcgcg
      481 cctgttcgag atggagaact gcgagatcga gttccgcgac gaagccctgc gtgcggtcgc
      541 acgcaaggcc atggagcgca agaccggtgc ccgcggcctg cgcacgatcc tcgaacacgt
      601 gctgctcgac accatgtacg acctgccgag cctcgatgac gtgagccgcg tcgtcgtcga
      661 tgacagcgtc gtgcgtggcg atgccaagcc gttcatgatc tacgagaacg ccggcccgcg
      721 ccgcgctgct tcctcggact gatccccccg ccgtgccggg ggcggtcgat ctgcccccga
      781 gccccctgtg cgccggatgc cgtgtgtcag cacgcatccg gccgtcgcct tccggcctgc
      841 ccgcccgcgg acgagctgtt gccgccacgc cgaggattcg tgatggagcc caggccgctt
      901 accgacgatt ccgatcccct tccggactcg catccgatcc cgatcctgcc gctgcgcgac
      961 gtggtcgtgt acccgcacat ggtcatcccg ctgttcgtcg ggcgcgagaa gtcgatcgcg
     1021 gcactcgatg cggcgatgca gaccgacaag cgcatcctgc tggtctcgca gaagagcgcc
     1081 gaggtcgatg atcccggtgt cgaggatctg taccgcatcg gcacgctgtc gaacatcctg
     1141 cagctgctga agctgccgga cggcaccgac aaggtgctgg tcgaaggcgg gcagcgggcg
     1201 cgcatcaccg gtttcagtga cggacacgcc tatttcgccg ccggcatcga gatcatcggt
     1261 gccgactacg aggagagccg cgagaccgag gtgctgatgc gctcgctgct cggcatcttc
     1321 gaccagtacg tgaagctcaa caagaagatc cccggcgagg tgctcagctc gctcgccggc
     1381 atcgaggacg ccgggcgcct ggccgacacg atcgccgcgc acatggcgct gaagctcgac
     1441 gagaagcaga agatcctcga gatcgagtcg gtcgatgccc ggctggagca cctgctgcat
     1501 ctggtcgaga gcgagatcga catcctgcag gtcgagaagc gcatccgcac gcgcgtcaag
     1561 cagcagatgg agaagagcca gcgcgagtac tacctcaacg agcagatgaa ggcgatccag
     1621 aaggagctgg gtgacctgga ggatgcaccg aacgaggccg acgaactcgc gcagaagatc
     1681 gaacaggccg gcatgcccaa ggaggccaag gccaaggcgg tcgccgagct gaacaagctc
     1741 aagatgatgt cgccgatgtc ggccgaggcg acggtcgtgc gcaattacat cgactggctg
     1801 gtgtcggtgc cgtggaagaa gcgcaccaag atccgccacg accttgccga ggccgagcgc
     1861 atcctcgaag ccgatcacta cggcctggag acggtcaagg aacgcatcct cgaatacctc
     1921 gccgtgcagc agcgcgccaa gaagctcaag gggccgatcc tgtgtctggt cggaccgccc
     1981 ggtgtcggca aaacctcgct tggccgctcg atcgcacgcg cgaccgggcg caagttcgtg
     2041 cgcatgagcc tcggcggcgt gcgcgacgag gccgagatcc gcggccaccg gcgcacctac
     2101 atcggttcga tgcccggcaa gatcgtgcag aacctctcga agatcgggac gcgcaatccg
     2161 ctgttcctgc tcgacgagat cgacaagatg tcgatggact ttcgcggcga cccgagttcg
     2221 gcgctgctgg aggtgctcga tccggaacag aacaacacct tcggcgacca ttacctggaa
     2281 gtcgactgcg acctgtcgga cgtgatgttc gtcgcgaccg ccaattcgct gaacattccg
     2341 gggccgctgc tcgatcgcat ggaggtcatc cggctgcccg ggtataccga ggacgaaaag
     2401 acccatatcg ccctcaaata cctggtgccg aaacaggtcg aggccaatgg cctgcatgcc
     2461 gatgaactgg aaatccgcga ggccgcggtt cgtgacatca tccgccatta cacgcgcgag
     2521 gccggtgtcc gcaatctgga gcgcgagatt tccaaaatct gccgcaaggc ggtcaagagc
     2581 atcctgctga aaccgcgcga ggaacgggcg cgcaccgcgg tgacgccgaa gacgctcgac
     2641 aaatatctcg gcgtgcgccg cttccgtttc gggctggccg aggatcagga ccagatcggc
     2701 catgtgaccg gactggcgtg gaccgaggtc ggcggtgaac tgctgaccat cgaagccgcg
     2761 ctgatgccgg gcaagggcaa gctgacctat accggccagc tcggcgaggt catgcaggag
     2821 tccatccagg cggcgatgac ggtcgtgcgc agccgctccg atgcgctcgg catcgatccg
     2881 gccttctttg aaaagcagga tctgcacatc cacgtgccgg agggagcgac gccgaaggat
     2941 ggcccgagtg ccggcacggc catgtgcacg gcgctggtct cggcactgac gcgcattccg
     3001 gtgcgcgcca acgtcgcgat gaccggagaa atcacgctgc gcggtgccgt gctgccgatc
     3061 ggcggactca aggagaaact gctggcggcg catcgcggcg gcattaccac ggttctgatt
     3121 ccggaagaca atcgcaagga tctggccgaa attcccaaga atattcttgc gaaaatcgaa
     3181 gtgcggccgg tgcaatggat tgatcaaatt ctggcccagg cgctcgtgca tctgccggag
     3241 ccttccaaac cggatgctga actaccggtc agtcaggaga cgccggcccg cagtccggct
     3301 gcttccgaac cggtgcgtcc gcactgaagc gctgttgaca cttccggaaa cgccaagcta
     3361 taacctcggc cacttcacag tggagcgggt ggcggctccg gatgtgccga cttctgttaa
     3421 ggccgtacgc catcggaaag cgccatcatt acaaccggta aatcatcagg tactcacagg
     3481 tacacataat gaataaatcg gaactgatcg atgccgttgc ggatgccacc ggaatcacga
     3541 agtccgacgc cggccggtcg gtggaggccg tgctcggcgc catcaccagt gctttgcagg
     3601 ggggcgatca ggtcacgctg gtgggcttcg gcaccttctc ggtgcgcgag cgggctgcac
     3661 ggaccggtcg tgacccgcgt tcagggcagg cgatcgagat caaggcttcc aaaaatcccg
     3721 tcttcaaggc tggcaaagcg ctgaaggatg ccttaaagta gcccgctctt ccggggggtg
     3781 cttagctcag ctgggagagc atcgccctta caaggcgagg gtcgggggtt cgaacccctc
     3841 agcacccacc aatgcagccg gaattccgga gcggtagttc agttggtcag aataccggcc
     3901 tgtcacgccg ggggtcgcgg gttcgagccc cgtccgctcc gccaactccg ctgcaggatg
     3961 taatacgagg taatccggat tcgggtcggc cattacctgg cgatcagatc cggtcatttc
     4021 gggaatgctc tgcggagcgg tagttcagtt ggtcagaata ccggcctgtc acgccggggg
     4081 tcgcggg
//
LOCUS       DAOQIE010000329         4083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13236_length_4083_cov_6.751490, whole genome shotgun sequence.
ACCESSION   DAOQIE010000329 DAOQIE010000000
VERSION     DAOQIE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13236_length_4083_cov_6.751490"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK144_16120"
     CDS             complement(<1..295)
                     /locus_tag="PK144_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67300.1"
                     /translation="MPPARLLPSLSVLLLAVSGCATLPPAPGAATIPAECTTLVTALD
                     AAAPGSPSTPRAVPGWPGLAADRFLAGFDPATLPAPAIDAWRRRLAGAGRGREA"
     gene            389..1531
                     /gene="earP"
                     /locus_tag="PK144_16125"
     CDS             389..1531
                     /gene="earP"
                     /locus_tag="PK144_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNF67301.1"
                     /translation="MQPILPSPALRWDLWCHVIDNYGDAGVCWRLARRLVAVHGQRVR
                     LWIDAPAVLARLWPAIDAQAARQVVAGVEIRHWRESLEAAECADAGDVVVEAFACELP
                     APARAALQARRSLWLNLEYLSAEAWVEGCHGLGSAQPGGRTRYFFFPGFTPATGGLIG
                     ADAACAQWDERAARAWLAAQGVTGPAAARRLSLFTYGNAALPGWLEALAAGDEPWLAL
                     VPDGRTLRALGDWLGAPLAPGETRTRAALSVHALPFLAQDDYDRLLWSSHLNVVRGED
                     SFVRAHWARRPLLWQAYRQDGEAHLDKLEAWLARALDGLEAGAAGAARAAHRAWNREA
                     DFAAHWPALRAALPALEVQAGRFAGRLAEGPELAAALVAFAQSKIE"
     gene            1566..2129
                     /gene="efp"
                     /locus_tag="PK144_16130"
     CDS             1566..2129
                     /gene="efp"
                     /locus_tag="PK144_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNF67302.1"
                     /translation="MKTAQEIRAGAVILVDGQPMVVQKAEFNKSGRNAAVVKMKLKNL
                     LSGSGTETVYKADEKFDVVILDKKECTYSYYADPMFVFMDAEYNQYEIERDDLGDAVH
                     YIVDGMEDVCEVTFYEGKVISMELPTIVVREVEYTEPAARGDTSGKVMKLARLKGSGY
                     ELQVPAFVETGEKIEIDTRTSEFRKRA"
     gene            complement(2210..3136)
                     /locus_tag="PK144_16135"
     CDS             complement(2210..3136)
                     /locus_tag="PK144_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67303.1"
                     /translation="MRASSTLEGRFAQIHVDAARNATDDFNPFHDPHHWQRIVANPFP
                     GPLVLGFQLEALIEYLWQTHPARDEDAAWAAAHGLHWTNWDLSFAGALCPGEAFTAEV
                     RAAARRETPEPGLSRRLLIRKADSLVLAGWLRQSAGALYEPGLSCAPFPGPLRAQPDR
                     CQRGPWFLKRKFASVGHAKNFLAGALADQALWFDELAPRSVFPQTYAAALLSCALLEQ
                     ATAAGHDFCAEPLVYTAHHLSILAPAVAGLRSNDALHLLVHGPELLAAGSGLGIGIEQ
                     TRYAGLVYAGTPERLLLRGELLLAPLSAITGG"
     gene            3209..3760
                     /locus_tag="PK144_16140"
     CDS             3209..3760
                     /locus_tag="PK144_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNF67304.1"
                     /translation="MREIEAGGVDVIIVFGAALAADGSPGPALLRRIEHAVALWQAGR
                     GRWLLCSGGLVAGTRSEAAVMAGCARAAGVPAQCIVEEDCARNTWQNAQFSAALLRRH
                     GWRTALLVTERYHLPRARLACRLNGLPVVAVSGPGHPPGRGRGRRLALALREGLACGM
                     MLMRAAAAILRRNGAESRQAAGD"
     gene            3878..>4083
                     /locus_tag="PK144_16145"
     CDS             3878..>4083
                     /locus_tag="PK144_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67305.1"
                     /translation="MSAGPGISGTDKSGLSAPVPAIPAHCVMAGVPVSSLLMVKGKNY
                     RAVSGNCRPIAGIRKRVHFHRKQNL"
BASE COUNT          592 a         1378 c         1553 g          560 t
ORIGIN      
        1 gcgcttcgcg cccgcgcccg gccccggcga ggcgccggcg ccaggcatcg atggccgggg
       61 ccggcagcgt cgccggatcg aaacccgcca gaaagcggtc agccgccagc cccggccagc
      121 cgggcacggc acgcggggtc gatggcgaac cgggcgcggc ggcatcgagc gccgtgacca
      181 gggtcgtgca ttcggccggg atcgtggccg cgccgggcgc gggcggcagc gtcgcgcagc
      241 cgctgacggc gagcagcagc acggacagcg agggcagcag gcgggcgggg ggcatgcagg
      301 aatctccggg gaggccggtg gcaggtacgc agccggcggg tcgcagcgat acagtgcccg
      361 gcctttgcgg cctgcggagt acaccggcgt gcagccgatc cttccatcac ccgccctgcg
      421 ctgggatctc tggtgccacg tcatcgacaa ctatggcgat gccggcgtct gctggcggct
      481 ggcgcgcagg ctggtcgcgg tgcacggcca gcgggtgcgg ctgtggatcg acgccccggc
      541 ggtgctcgcg cggctgtggc cggcgatcga cgcgcaggcg gcgcgccagg tcgtggccgg
      601 cgtcgagatc cggcactggc gggaatctct ggaagcggcc gagtgcgccg atgccggcga
      661 cgtggtcgtc gaagccttcg cctgcgaact gccggcgccc gcgcgtgccg cgctgcaggc
      721 gcgccggtcg ctgtggctga acctcgaata cctgtcggcc gaagcctggg tcgagggctg
      781 ccacgggctc ggcagtgcgc agccgggcgg gcggacgcgc tacttcttct ttcccggctt
      841 cacgccggcg accggcggcc tgatcggcgc cgatgcggcg tgcgcgcagt gggatgagcg
      901 ggccgcgcgc gcgtggctgg ccgcgcaggg cgtgacgggg ccggcggcgg cgcggcggct
      961 gtcgctgttc acgtacggca atgccgcgct gccgggctgg ctggaggcgc tcgcagcggg
     1021 cgatgagccg tggctcgcgc tggtgccgga cgggcgcacg ctgcgtgcgc tcggcgactg
     1081 gctcggggcg ccgcttgcgc ccggcgaaac gcgcacgcgc gctgcgctga gcgtccacgc
     1141 gctgcccttc ctcgcgcagg acgactacga ccggctgctg tggagcagcc atctcaacgt
     1201 cgtgcgcggc gaggacagct tcgtgcgcgc gcactgggcg cgccggccgc tgctgtggca
     1261 ggcctaccgg caggacgggg aggcgcacct cgacaagctc gaagcctggc tcgcgcgggc
     1321 gctggacggg ctggaggccg gggccgccgg cgctgcgcgc gcagcgcacc gggcctggaa
     1381 ccgggaggcg gatttcgcgg cgcactggcc ggcgctgcgc gcggcgctgc cggcgctgga
     1441 ggtgcaggcc gggcgttttg ccgggcggct ggcggaaggg ccggagcttg cggccgcgct
     1501 ggtggcgttc gcgcaatcga agatagaata agcagttttt acacccaacc caccaggatc
     1561 acccgatgaa aaccgctcag gaaatccgcg ccggcgccgt cattctggtc gacggtcagc
     1621 cgatggtcgt gcagaaggct gagttcaaca agtccggccg caacgccgcg gtcgtgaaga
     1681 tgaagctgaa gaacctgctg tcgggttccg gcaccgagac ggtctacaag gccgacgaga
     1741 agttcgatgt cgtcatcctc gacaagaagg aatgcacgta ctcgtactac gccgatccga
     1801 tgttcgtgtt catggacgcc gaatacaacc agtacgagat cgagcgcgac gatctcggcg
     1861 atgccgtcca ctacatcgtc gacggcatgg aagatgtctg cgaagtgacc ttctacgaag
     1921 gcaaggtcat ctcgatggaa ctgccgacca tcgtcgtgcg cgaggtcgaa tacaccgagc
     1981 cggccgcacg cggcgacacc tcgggcaagg tcatgaagct cgcgcgcctc aagggcagcg
     2041 gctacgaact gcaggtgccg gccttcgtcg agaccggcga gaagatcgaa atcgacacgc
     2101 gcaccagcga gttccgcaag cgcgcgtgaa gccggtcacc cccgccggat gtgtccgccc
     2161 tcgcccccgg tcgccgtccg cgatcggggg cgatgcgtct gggcagccgc tatccgccgg
     2221 tgatcgccga cagcggcgcg agcagcagtt cgccacgcag cagcaggcgc tccggcgtgc
     2281 cggcgtagac cagcccggcg tagcgcgtct gctcgatgcc gatgccgagc ccgctgccgg
     2341 ccgcgagcag ctcggggccg tgcacgagca ggtgcagcgc atcgttgctg cgcagcccgg
     2401 ccacggccgg cgcgaggatg ctcaggtgat gcgcggtata cacgagcggt tcggcgcaga
     2461 agtcatgccc ggccgccgtc gcctgttcga gcagcgcaca cgagagcagc gccgcggcgt
     2521 aggtctgcgg gaacaccgag cgcggcgcca gttcgtcgaa ccacagcgcc tggtcggcca
     2581 gcgcgccggc gaggaagttc ttggcgtggc cgacgctcgc gaacttgcgc ttcaggaacc
     2641 aggggccgcg ctggcagcgg tcgggctgcg cgcgcagcgg cccggggaag ggcgcgcagc
     2701 tcaggcccgg ctcgtacagc gccccggcgc tctggcgcag ccagccggcc agcaccaggc
     2761 tgtcggcctt gcggatcagc agccggcgcg acagccccgg ctccggagtc tcgcgccggg
     2821 ccgccgcgcg cacttcggcc gtgaacgcct cgcccggaca cagcgcgccg gcgaaggaca
     2881 gatcccagtt cgtccagtgc aggccgtgcg ccgcggccca ggccgcgtcc tcgtcacggg
     2941 ccggatgggt ctgccacaga tactcgatca gggcttcgag ctggaagccg agcaccagcg
     3001 gacccgggaa cgggttggcg acgatgcgct gccagtggtg cggatcgtgg aagggattga
     3061 agtcgtcggt ggcattgcgc gcggcatcca cgtggatctg ggcaaaccgg ccttcgagcg
     3121 tcgagctggc gcgcatcgcg gactccgggc ctgcgccgcg ccggcggact gccggtgcgc
     3181 gtgcggccgt cgccgagctt agtcgcgcat gcgtgagatc gaagccgggg gagtcgacgt
     3241 catcatcgtg ttcggtgcgg cgctggcggc ggacggctcg ccggggccgg cgctgctgcg
     3301 gcgcatcgaa cacgccgtcg cgctgtggca ggccgggcgc gggcgctggc tgctgtgctc
     3361 gggcggactc gttgccggca cgcgcagcga agccgcggtg atggccggat gcgcgcgcgc
     3421 tgccggagtg ccggcgcagt gcatcgtcga ggaggactgc gcgcgcaaca cctggcagaa
     3481 cgcgcagttc agcgcagcac tgctgcgcag gcatggctgg cgcacggcgc tgctggtgac
     3541 cgagcgctat cacctgccgc gcgcccggct cgcctgccgg ctgaacgggc tgccggtcgt
     3601 tgccgtcagc ggcccggggc atccgcccgg acgcggccgg ggccggcgcc tcgccctggc
     3661 gctgcgtgag ggactcgcct gcgggatgat gctcatgcgg gctgccgcgg ccattctgcg
     3721 ccgtaacggt gcagaaagcc ggcaggccgc cggcgattga acgggtgatc gtcccgacac
     3781 cggtactgcg catgacgatt gccggcaatc tcccgggcgc cggcagccgg tcgacaccgg
     3841 cggtttcggc aattgcacca ggttggcgca aatcagcatg agcgctggcc cgggcatttc
     3901 cggtacggac aaatccggtt tgtccgcacc ggtcccggca ataccggcgc actgcgtcat
     3961 ggccggtgtg ccggtgtcca gcctgctgat ggtaaagggg aaaaattacc gcgccgtgtc
     4021 cgggaattgc cggccaatag ccggcatccg gaagcgcgtg cattttcatc ggaagcagaa
     4081 tct
//
LOCUS       DAOQIE010000330         4069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13321_length_4069_cov_8.241654, whole genome shotgun sequence.
ACCESSION   DAOQIE010000330 DAOQIE010000000
VERSION     DAOQIE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13321_length_4069_cov_8.241654"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1607
                     /gene="gatA"
                     /locus_tag="PK144_16150"
     CDS             153..1607
                     /gene="gatA"
                     /locus_tag="PK144_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNF67306.1"
                     /translation="MHTQTLAELARGLAAGAFSSRELTEALLARIQAHDGALNSFITV
                     TPERALAQADAADAARARGEYGALTGLPIAHKDIFCTQGVKTSCGSKMLDRFVAPYDA
                     TVVAKLDAAGMVMLGKTNMDEFAMGSSNESSWYGPVRNPWDVRAVPGGSSGGSAAAVA
                     ARLLPAATGTDTGGSIRQPAALCGITGLKPTYGRVSRWGMIAYASSLDQGGPLARTAE
                     DCALLLGAMAGFDARDSTSAGAPVPDYRAGLEAGLDGLRIGLPKEYFGAGLDAGVARA
                     VEAAIDEYRRLGADVVEVSLPNAQLAIPAYYVIAPCEASSNLARYDGVRFGHRCEAPR
                     DLVDLYTRSRAEGFGAEVQRRILVGTYALSAGYYDAYYLKAQQIRRLIHEDFRRAFEH
                     CDLIAGPTTPDVAFDLGEKADDPVALYLADIYTVAVNLAGLPALSHPVGDKGGRPVGL
                     QLIGPAFAEARLLNAAHRFQLATDWHRRAPQGFA"
     gene            1796..3241
                     /gene="gatB"
                     /locus_tag="PK144_16155"
     CDS             1796..3241
                     /gene="gatB"
                     /locus_tag="PK144_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNF67307.1"
                     /translation="MEWEAVIGLEVHTQLATKSKIFSGAATAYGAEPNRQACAIDLGL
                     PGVLPVLNAEAVRMAVMLGLAIDATVARRSVFARKNYFYPDLPKGYQISQYELPVVAR
                     GHLDIQIDGVAKRIGITRAHLEEDAGKSLHEDFHGLSGIDLNRAGTPLLEIVSEPDLR
                     SAQEAVAYLKKLHQLVVWLGICDGNMQEGSFRCDANVSVRPKGQEQFGTRAEIKNVNS
                     FRFVERAIEYEIERQIELIEGGGRVVQETRLYDPDRDETRSMRGKEEANDYRYFPDPD
                     LLPLVIEDSFIEQLRASLPELPQAAFARFVRDYGLPDYDAATLTAERATAGYYEALVA
                     ATGGEARLCAKLCANWVMGDLAAALNRAGLPIGESPVPAEALAGLLGRVLDGTLSGKL
                     AKEVFAAMWAGEGTADAIIAARGLVQLTDSSAIDTLIDGILAASPKQVEQYRAGQDKL
                     FGFFVGQVMKASKGKANPQQVNERLLARLKG"
     gene            3365..4069
                     /locus_tag="PK144_16160"
     CDS             3365..4069
                     /locus_tag="PK144_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67308.1"
                     /translation="MTDRARTVRRPRWPLVLLPLLPLLLAATYNIRDRMSHGSWASFM
                     MYLGDDVLFRAAVTRGQDTFAVDSKLNTCDKFQLKVIGTLSKPSSDNTEFGAAVLIRV
                     DEAGLHQTSGQFSAERGSTTAFLLINRVEEGPRLFREMKYGNTVRFKVQPEGNNSPTF
                     MSFPLNGSAAAISRADDLCARYSNASGGGGGRGRNDGGGAPDDSDYFGGGGGGGQPQP
                     QPQPQRPKPRGDDSFF"
BASE COUNT          627 a         1470 c         1373 g          599 t
ORIGIN      
        1 gcagcgggcg tcgcggaaag gtgcagggag acgctgcgga tttcctgcct cggccctgtc
       61 caggtcctgc gctgcccgcg tctccccccg cgcgcctgac gcctcatccg ctcctgcgcc
      121 gtcccccgtc cccgaccggg aagtgaatgc ccatgcacac ccagaccctc gccgaactgg
      181 cccgcggcct cgccgccggc gcgttctcgt cgcgtgagct gaccgaggcg ctgctcgcgc
      241 gcatccaggc gcatgacggc gcgctgaaca gcttcatcac cgtcacgccc gagcgcgcgc
      301 tcgcgcaggc cgatgccgcc gatgccgcgc gtgcgcgggg cgaatatggc gcgctgaccg
      361 gcctgccgat cgcgcacaag gacatcttct gcacgcaggg cgtgaagacc tcctgcggct
      421 cgaagatgct cgatcgcttc gtcgcgccct acgacgccac cgtggtcgcg aagctcgatg
      481 ccgccggcat ggtcatgctc ggcaagacca acatggacga gttcgcgatg ggctcgtcga
      541 acgagtcgag ctggtacggt ccggtgcgca atccctggga cgtacgggcg gtgccgggcg
      601 gttcgtccgg cggctcggcg gcagcggtcg ccgcgcgcct gctgccggcg gcgaccggca
      661 ccgacaccgg cggctcgatc cgccagccgg ccgcgctctg cggcatcacc ggcctcaagc
      721 ccacgtacgg gcgcgtgtcg cgctggggca tgatcgccta cgcctccagc ctcgatcagg
      781 gcggaccgct cgcgcgcacc gccgaggact gcgcgctgct gctcggcgcg atggccggct
      841 tcgatgcgcg cgactcgacc agcgccggag caccggtgcc ggactatcgc gccggcctcg
      901 aagccgggct cgacggcctg cgcatcggcc tgccgaagga atacttcggt gccggcctcg
      961 atgccggcgt cgcgcgcgcc gtcgaggcgg cgatcgacga ataccgccgg ctcggcgccg
     1021 acgtggtcga ggtcagcctg ccgaacgcgc agctggcgat cccggcctac tacgtgatcg
     1081 cgccctgcga ggcatcgagc aacctcgcgc gctacgacgg cgtgcgcttc ggtcaccgct
     1141 gcgaagcacc gcgcgacctg gtcgatctct acacgcgctc gcgcgccgag ggtttcggtg
     1201 ccgaggtgca gcggcgcatc ctggtcggca cgtatgcgct gtcggccggt tactacgatg
     1261 cctattacct gaaggcgcag cagatccgcc ggctgatcca cgaggacttc cgccgcgcct
     1321 tcgagcactg cgacctgatc gccggtccga ccacgccgga tgtcgccttc gatctcggcg
     1381 agaaggccga tgatccggtg gccctgtatc tggccgacat ctacacggtc gcggtcaacc
     1441 tcgccggcct gccggcgctg tcgcacccgg tcggtgacaa gggcgggcgg ccggtcggcc
     1501 tgcagctgat cggtccggcc ttcgccgaag cgcgcctgct gaacgccgcg caccgcttcc
     1561 agctcgccac cgactggcac cggcgcgcgc cgcagggctt cgcgtgaggg gagcgtcagg
     1621 ggatgaagcg tgatcggcga tgagggcggt ggggaatcgg gcggccgcac cggcgtctgc
     1681 cggtgccgcg gcttcgcgcc gatccctgtc ctgctgagcg ccgcctgcca gtcatccccg
     1741 cgcaggtccc gggcctgatc cctcactcct tcaaactcac tccaggtacg cacggatgga
     1801 atgggaagca gtcatcgggc tggaggtgca cacccagctc gcgacgaaat cgaagatctt
     1861 ctcgggcgcg gccaccgcct acggcgccga gccgaaccgt caggcctgcg cgatcgacct
     1921 cggcctgccc ggcgtgctgc cggtgctcaa cgccgaggcc gtgcgcatgg cggtgatgct
     1981 cgggctggcg atcgacgcga cggtcgcgcg gcgcagcgtg ttcgcgcgca agaattactt
     2041 ctatccggac ctgccgaagg gctaccagat ctcgcagtac gagctgccgg tggtcgcccg
     2101 cggtcatctc gatatccaga tcgacggcgt ggcgaagcgc atcggcatca cccgcgcgca
     2161 tctggaggag gacgccggca agtcgctgca cgaggacttc cacggcctct ccggcatcga
     2221 cctgaaccgc gccggcacgc cgctgctgga gatcgtctcc gagcccgacc tgcgctcggc
     2281 gcaggaagcc gtcgcctacc tgaagaagct gcaccagctc gtggtctggc tcggcatctg
     2341 cgacggcaac atgcaggaag gctcgttccg ctgcgacgcc aacgtctcgg tgcgcccgaa
     2401 gggacaggag caattcggca cgcgtgccga gatcaagaac gtcaacagct tccgcttcgt
     2461 cgagcgcgcg atcgaatacg agatcgagcg ccagatcgag ctgatcgagg gcggcggccg
     2521 cgtcgtgcag gaaacccggc tctacgaccc ggatcgcgac gaaacccgtt cgatgcgcgg
     2581 caaggaagaa gccaacgact accgctactt cccggacccc gacctgctgc cgctcgtgat
     2641 cgaggacagc ttcatcgaac agctgcgggc gagcctgccc gaactgccgc aggccgcctt
     2701 cgcgcgcttc gtgcgcgact acggcctgcc tgactacgat gccgcgacgc tgaccgccga
     2761 gcgcgctacc gccggctact acgaggcgct ggtcgccgcg accggcggcg aggccaggct
     2821 ctgcgcaaag ctctgtgcca actgggtcat gggcgatctg gccgcggcgc tgaaccgcgc
     2881 cggcctcccg atcggcgaaa gcccggtgcc ggccgaagcg ctcgccgggc tgctcgggcg
     2941 cgtgctcgac ggcacgctct ccggcaagct cgccaaggaa gtgttcgctg cgatgtgggc
     3001 gggcgagggc acggccgatg cgatcatcgc ggcgcgcggg ctggtgcagc tcaccgacag
     3061 ctcggcgatc gacacgctga tcgacggcat cctcgcggcc agcccgaagc aggtcgagca
     3121 gtaccgcgcc ggtcaggaca agctgttcgg cttcttcgtc ggccaggtca tgaaggcctc
     3181 gaagggcaag gccaacccgc agcaggtcaa cgagcggctg ctcgcgcggc tgaagggctg
     3241 atcaccggta cactgactgt cagcgggccc cgggcaggac atcgcccgga cccgctcagc
     3301 caacctaata caggcggaca tccgccttca gaaccgattc cggaccaccg gccgggggga
     3361 ctccatgacc gatcgtgcgc gcaccgtccg ccggccacgc tggccgctcg tgctgctgcc
     3421 gctgctgccg ctgctgctgg cggcgaccta caacatccgc gaccgcatga gtcacggcag
     3481 ctgggcctcg ttcatgatgt acctcggcga cgacgtgctg ttccgcgccg cggtcacgcg
     3541 cggccaggac accttcgcgg tcgattccaa gctcaacacc tgcgacaagt tccagctcaa
     3601 ggtcatcggc acgctgtcga agccctcgtc ggacaacacc gagttcggcg ccgccgtgct
     3661 gatccgcgtc gacgaggccg gcctccacca gacctcgggc cagttcagcg ccgagcgcgg
     3721 cagcaccacg gccttcctgc tgatcaaccg cgtcgaggaa ggcccgcggc tgttccgcga
     3781 gatgaagtac ggcaacacgg tccgcttcaa ggtgcagccc gagggcaaca actcgccgac
     3841 cttcatgagc ttcccgctga acggctcggc ggcggcgatc tcgcgcgccg acgacctctg
     3901 tgcccgctat tcgaatgcca gcggcggcgg tggtggtcgt ggccgcaacg acggcggcgg
     3961 tgcgccggat gattccgact acttcggtgg cggtggtggc ggcggtcagc cgcaacccca
     4021 gccgcagccg cagcgtccga aaccgcgtgg cgacgacagc ttcttctga
//
LOCUS       DAOQIE010000331         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13512_length_4036_cov_6.632504, whole genome shotgun sequence.
ACCESSION   DAOQIE010000331 DAOQIE010000000
VERSION     DAOQIE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13512_length_4036_cov_6.632504"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK144_16165"
     CDS             complement(<1..496)
                     /locus_tag="PK144_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNF67309.1"
                     /translation="MSTVASDVPAATTGGGSALDLARALILAPAGLDEPRLESALGTL
                     LGHAVDYGDLYFQVRRAESWSLEDGAVKSAAHNIDQGVGVRAIAGERTGFAYSDELHA
                     PALLEAAKAARAIAASGKGPERGVRIAGTRPATALYAPLDPLGTLSADEKIRLLEAVD
                     AEARR"
     gene            complement(730..1539)
                     /locus_tag="PK144_16170"
     CDS             complement(730..1539)
                     /locus_tag="PK144_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNF67310.1"
                     /translation="MSRIALIQMCAGADPAANCATAGRLVAEAAAAGAGLVALPESWF
                     CIDADPAAGLRLAEDEGRGPLQAFLAGLAARHGVWLLGGTLPLRGEEPDRVCAAALLF
                     DARGRIGARYDKVHLFDVDVADAQGRYRESRVVEPGERIVVADTPFGRLGLTVCYDLR
                     FPELFRAMSAAGAEVFVVPSAFTAVTGRAHWETLLRARAIENLAWVLAPAQCGTHPGG
                     RETHGDSLVVEPWGEVIARRASGEGIVLAEIDLERGRRLRAEFPCLDHRRL"
     gene            1715..4030
                     /locus_tag="PK144_16175"
     CDS             1715..4030
                     /locus_tag="PK144_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF67311.1"
                     /translation="MKLRSLQLKIAGLAGLCLLGTAIAMVGFATLSNRSSEAFVQARV
                     GDLLERGTRSSLQALARAQAGLIRSELDSAFGAARNMARAFEQIATNDTAVATPPALR
                     RAQLDGILLGVLKAHPRFNGTYSAWEPDGLDGLDAEAAAGHRAGTDASGRFLSYWTRD
                     SSGHIAVQPLVEYDSRERHPNGVIKGAWYLGPQANGQESILGPLPYIVQGKSVYLATM
                     SVPVSIGGRFRGVAGADFDLAFVQKLAEQVDASVFKGQGSVAIVSNDGLVVAASDQPG
                     LIGSSFRPLVGKDHDIDLTVVRDGRSHVVLDETSGLLKFFEPITLGYTKTPWSVLITV
                     PQTVAMAEAASLAAALHERAGHDVLMQTLVALAILVLGVGTMWLVARSITRPILASAR
                     FAGEIAAGRLDSELHVESADETGQLAEALRTMATDIRRSNAERAAAQAAAEREREQRA
                     RAAEVERQEQERRAEQERNERLQREEAERIERQKTLEAERRATLARMADELEAGIRAV
                     TGELTRAAASMKSASAGLAQSADHSRAQAQQAARAAARADENAQTVASATEELSASIR
                     EIARQVDKSTAICSQAVESVGRADHQVLGLTAVADKIGAIVGLITSIAEQTNLLALNA
                     TIEAARAGEAGKGFAVVATEVKELAKQTTSATQGIAQQVAEVQRVVKDTAAEIRGIGG
                     VIGETRSVVTTIATAVTEQSAATTDIARNVAEAAGSVRSTSDGIAAVTRATDASRAAT
                     GAVDEAAGSLSSEVDRLNTIVEELLRKMRSA"
BASE COUNT          628 a         1551 c         1376 g          481 t
ORIGIN      
        1 ggcggcgcgc ctcggcatcg acggcttcga gcaggcggat cttctcgtcg gcgctcagcg
       61 tgccgagcgg atcgagcggc gcgtacagcg ccgtcgccgg ccgggttccg gcgatgcgca
      121 cgccgcgctc cgggcccttg ccgctcgcgg cgatcgcacg cgcagccttc gcggcttcga
      181 gcagcgccgg cgcgtgcagc tcgtccgaat acgcgaagcc ggtgcgctcg ccggcgatcg
      241 cgcgcacgcc gacgccctgg tcgatgttgt gcgcggccga cttcaccgcg ccgtcctcca
      301 gcgaccagga ctcggcgcgg cgcacctgga agtacaggtc accgtaatcg accgcatggc
      361 cgagcagcgt gccgagcgcc gattcgagcc gcggctcgtc gagcccggcc ggcgcgagga
      421 tcagggcccg cgccagatcg agcgcggaac cgccgccggt ggtggcggcg ggtacgtcgg
      481 atgcaacggt gctcatggcg gggacctcgg ggggcatcgg ggcagttgtt cgtcaaggta
      541 acgcgaagcg cgcatgcgcc gcgagcctgc atggcaggcg catgacagcc atgtggcgcc
      601 gtgttgacgg cggcatcgcc cggctgtccg gcaagccgct gcgggacggc cgggggtgca
      661 ccgccgcagc cggtgcgccg tctgccgtgc atccgccggc gcagcgcggc gcgtcgccac
      721 gccggccggc tacagccgcc ggtgatcgag gcacggaaac tccgcacgca gccgccgccc
      781 gcgttcgagg tcgatttcgg cgagcacgat gccctcgccg ctcgcgcggc gcgcgatcac
      841 ctcgccccag ggctcgacga cgaggctgtc gccgtgcgtc tcgcgcccgc ccggatgcgt
      901 gccgcactgc gccggggcca gcacccaggc gaggttctcg atcgcgcggg cgcgcagcag
      961 ggtctcccag tgcgcgcggc cggtgacggc cgtgaacgcc gagggcacga cgaacacctc
     1021 ggcgccggcg gcgctcatcg cccggaaaag ctccgggaag cgcaggtcgt agcacaccgt
     1081 cagcccgagc cgcccgaacg gcgtatcggc gacgacgatg cgctcgccgg gctcgacgac
     1141 ccgggattcc cggtagcgcc cctgcgcatc ggccacatcg acatcgaaca ggtgcacctt
     1201 gtcgtagcgc gcaccgatgc gcccgcgcgc atcgaagagc agcgccgccg cgcagacgcg
     1261 atcgggctcc tcgccgcgca gcggcagcgt gccgccgagc agccagacgc cgtgacgcgc
     1321 cgccagcccg gccagaaacg cctgcagcgg cccgcggcct tcgtcctcgg ccagccgcag
     1381 gccggcggcc ggatcggcat cgatgcagaa ccagctctcg ggcagcgcca ccagcccggc
     1441 accggctgca gcggcctcgg cgaccagccg gccggcggtc gcgcagttgg cggccggatc
     1501 ggcaccggca cacatctgga tcagggcgat acggctcatc ggcggggaat cctcggcagg
     1561 atcggcatgc cgcaatgcag caatgcccct gcatgttgca atgttttccg caaaccttgc
     1621 gccaaaggta ttcagggcag cgaccggcag ccgacaagat ggcggaccca cgtcatccgt
     1681 ctccgtgccc gaacgcgcgc caaggacaag ccctgtgaaa ctgcgctccc tgcaactgaa
     1741 gattgccggc ctcgccggac tgtgcctgct cggaaccgcc attgcgatgg tcggtttcgc
     1801 gaccctatcc aaccgcagtt ccgaagcctt cgtgcaggcg cgggtcggcg acctgctcga
     1861 acgcggcacg cgcagcagcc tgcaggcact ggcgcgggcg caggccgggc tgatccgctc
     1921 cgaactcgac tccgccttcg gcgccgcgcg caacatggcc cgcgccttcg agcagatcgc
     1981 caccaatgac accgccgtcg ccacgccgcc ggcgctgcgc cgtgcccagc tcgacggcat
     2041 cctgctcggc gtgctgaagg cccatccgcg cttcaacggc acctacagcg cctgggagcc
     2101 ggacggcctc gacggcctcg acgccgaagc cgccgccggg catcgcgccg gcaccgacgc
     2161 cagcggccgc ttcctgtcct actggacgcg cgacagcagc ggccacatcg ccgtgcagcc
     2221 gctggtcgag tacgacagcc gcgagcgcca cccgaacggc gtcatcaagg gcgcgtggta
     2281 tctcggcccg caggccaacg gccaggaaag catcctcggc ccgctgccct acatcgtgca
     2341 gggcaagtcc gtgtatctcg ccaccatgtc ggtgccggtc agcatcggcg gacgcttccg
     2401 cggcgtcgcc ggtgccgact tcgatctcgc cttcgtgcag aagctcgccg agcaggtcga
     2461 tgcctcggtg ttcaagggcc agggttcggt ggcgatcgtc agcaacgacg gcctcgtcgt
     2521 cgccgccagc gaccagcccg ggctgatcgg cagttcgttc cggccgctgg tcggcaagga
     2581 ccacgacatc gacctgaccg tggtgcgcga cggccgcagc cacgtcgtcc tcgacgaaac
     2641 cagcggcctg ctcaagttct tcgagccgat cacgctcggc tacaccaaga cgccgtggtc
     2701 ggtgctgatc accgtgccgc agacggtcgc gatggccgaa gccgccagcc tcgccgccgc
     2761 cctgcatgag cgggccggcc acgacgtgct gatgcagacg ctggtggcgc tggcgatcct
     2821 cgtgctcggc gtcggcacga tgtggctggt ggcgcgcagc atcacccggc cgatcctcgc
     2881 cagcgcccgc ttcgccggcg agatcgccgc cgggcgcctc gacagcgagc tgcacgtcga
     2941 atcggccgac gaaaccggcc agctcgccga ggcgctgcgc acgatggcca ccgacatccg
     3001 ccgcagcaac gccgagcgcg ccgccgcgca ggccgccgcc gagcgcgagc gcgagcagcg
     3061 cgcccgcgcc gccgaggttg aacgccagga acaggaacgc cgcgccgagc aggagcgcaa
     3121 cgagcgcctg cagcgcgagg aggccgaacg catcgagcgc cagaagacgc tcgaagccga
     3181 acgccgcgcc acgctcgcgc gcatggccga cgaactcgaa gccggcatcc gcgccgtcac
     3241 cggcgagctg acgcgcgccg ccgccagcat gaagagcgcc tctgccggcc tcgcgcagtc
     3301 cgccgaccac tcgcgcgccc aggcccagca ggccgcccgc gccgccgccc gcgccgacga
     3361 gaacgcccag accgtcgcct cggccaccga ggaactgtcg gcctcgatcc gcgagatcgc
     3421 ccgtcaggtc gacaagtcga cggcgatctg cagccaggcg gtcgaatcgg tcgggcgcgc
     3481 cgatcatcag gtgctcggcc tgaccgcggt cgccgacaag atcggcgcga tcgtcggcct
     3541 gatcacctcg atcgccgagc agaccaatct gctcgcgctc aacgccacca tcgaggcggc
     3601 gcgcgccggc gaggccggca agggcttcgc cgtcgtcgcc accgaggtca aggaactcgc
     3661 caagcagacc acctccgcca cccagggcat cgcccagcag gtcgccgagg tgcagcgcgt
     3721 ggtcaaggac accgccgccg agatccgcgg catcggcggc gtcatcggcg agacgcgcag
     3781 cgtcgtcacc accatcgcca ccgccgtcac cgagcagtcc gccgccacta ccgatatcgc
     3841 ccgcaacgtc gccgaggccg ccggcagcgt gcgcagcacc agcgacggca tcgccgccgt
     3901 cacccgggcc accgacgcct cgcgcgccgc caccggcgcc gtcgacgaag ccgccggcag
     3961 cctgtcgtcc gaagtcgacc gcctcaacac catcgtcgag gaactgctgc gcaagatgcg
     4021 ctcggcctga agcacc
//
LOCUS       DAOQIE010000332         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13608_length_4018_cov_6.583396, whole genome shotgun sequence.
ACCESSION   DAOQIE010000332 DAOQIE010000000
VERSION     DAOQIE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13608_length_4018_cov_6.583396"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..323)
                     /locus_tag="PK144_16180"
     CDS             complement(3..323)
                     /locus_tag="PK144_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67312.1"
                     /translation="MEARFTSMEAGVRLLHLRVRLLNSWVRLLNLRVRLLHLRVKLLN
                     SWVKLLNLRVRLLNLQVKLLHLRVKLLNSRVKLLHPRVKLLHPRVRLFDVLLGLMNPR
                     VSAA"
     gene            complement(557..1255)
                     /gene="cybH"
                     /locus_tag="PK144_16185"
     CDS             complement(557..1255)
                     /gene="cybH"
                     /locus_tag="PK144_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007019930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HNF67313.1"
                     /translation="MSESIEVIRAPQVVYVYEAPVRLWHWVNALAILVLGVTGYFIGS
                     PLPSLPGEASHSFLMGYLRFAHFAAGYVLAIGFLGRIYWAIVGNHHAHQLFVLPFWNL
                     QWWKEVIWEARWYGFLEKIPKKYVGHNPLAQLAMFTLFTLGITFMICTGFALYAEGAQ
                     QGSWADHVFGWVIPLFGQSQDVHTWHHFGMWVIVIFVILHVYAAIREDIMSRQSMVST
                     MVSGYRMFKDDRPD"
     gene            complement(1320..1907)
                     /locus_tag="PK144_16190"
     CDS             complement(1320..1907)
                     /locus_tag="PK144_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNF67314.1"
                     /translation="MSSEMMRKLLMLFLLAAVIPAAHAHPFHAEASGFPAGLAHPLGG
                     LDHLLAMLAVGLWSAWAVPQQKFVLPLAFLTAMAGGATLALSGLTLPFVEPGIGVSVL
                     VLGLLLAGLVRLPLAAGAGLVSVFALFHGVAHGAGLPEAASPYGYAAGFLLATGLLHL
                     AGIAIGTRLAQQRTWWLRAIGTAMGLSGLWLLAGA"
     gene            complement(1962..3758)
                     /locus_tag="PK144_16195"
     CDS             complement(1962..3758)
                     /locus_tag="PK144_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNF67315.1"
                     /translation="MAVINTPNGFRLDAAGTRVVVDPVTRIEGHMRCEVNLDANNVIT
                     NAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGTHALTSVRAVEDALGIQIPEN
                     ANSIRNLMQLTLQAHDHLVHFYHLHALDWVNPVNALKADPKATSEIAQKISAWPLSSP
                     GYFRDIQTRLKKFVESGQLGLFRNGYWSNPAYRLPAEVDLLAVAHYLEALDFQREIVK
                     IHTIYGGKNPHPNWLVGGMPCAINVHTTGAVGAINMERLNHVSSIIDRTREFIDQVYL
                     PDLMAIASFYKDWTYGGGLSSKAVMSYGDIPERANDYGAANLKLPHGAIIGGDLRNIQ
                     NVDVRDPAEVQEFVDHSWYRYPEGAGKGRHPWEGVTEPDFTLGPNTKGTKTRIEALDE
                     SAKYSWIKAPRWKGHAVEVGPLARYIIGYAQNRPEFREPVDKALRDLGLPLEALFSTV
                     GRTLARGLEASWAAAEMRHFQDKLVATIKAGDESTANVEKWDPSTWPKSAKGVGFTEA
                     PRGALGHWVRIEDGRIESYQAVVPTTWNASPRDVQGNIGAYEAALLNTPVARADEPVE
                     ILRTLHSFDPCLACSTHVMGPDGEELSTVRVR"
     gene            complement(3761..>4018)
                     /locus_tag="PK144_16200"
     CDS             complement(3761..>4018)
                     /locus_tag="PK144_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uptake hydrogenase small subunit"
                     /protein_id="HNF67316.1"
                     /translation="NEGTSFPIQSGHGCIGCSEDGFWDKGSFYDRLTGIQQFGVEQNA
                     DRVGATAAGVVGAAVAAHAAVSAVTRARVKHVQTDERREDR"
BASE COUNT          682 a         1299 c         1374 g          663 t
ORIGIN      
        1 ggctacgcag cgctgacccg cgggttcatc agaccaagca gcacatcaaa gagcctgacc
       61 cgcgggtgta agagcttgac ccgcgggtgt aagagcttga cccgcgagtt taagagcttg
      121 acccgcaggt gtaagagctt gacctgcagg tttaagagcc tgacccgcag gtttaagagc
      181 ttgacccacg agtttaagag cttgacccgc aggtgtaaga gcctgacccg caggtttaag
      241 agcctgaccc acgagtttaa gagcctgacc cgcaggtgta agagcctgac cccggcttcc
      301 atcgacgtga accgcgcttc cacgagcgtg tcccgcacgg tcacgccctg agcgcaacgg
      361 cagcgtcggg cagatcgtcc ggtttgcgcg tgcgttcgtc cggccgtcat caccatcggc
      421 gctgccgcgg cctgcacgca taaaaaacgg cgcatccggg aaatccggat gcgccgaggg
      481 ctgctccggt ccgtgtccga agcggacccg gagccgaggg ggagtcgtgg ttacctcaga
      541 gtctcatcga gggctttcag tccggacggt cgtccttgaa catccggtag ccgctgacca
      601 tggtcgagac catgctctgg cgcgacatga tgtcttcgcg gatcgccgcg tagacgtgca
      661 ggatcacgaa gatcacgatc acccacatgc cgaagtgatg ccaggtgtgc acgtcctgac
      721 tctggccgaa cagcgggatg acccagccga acacgtgatc ggcccacgaa ccctgctgcg
      781 caccttcggc atagagcgca aagccggtgc agatcatgaa cgtgatgccg agcgtgaaca
      841 gcgtgaacat cgcgagctgc gcgagcgggt tgtggccgac gtacttcttc gggatttttt
      901 ccaggaagcc gtaccagcgc gcctcccata tcacttcctt ccaccactgc aggttccaga
      961 acggcagcac gaacagctga tgcgcgtggt ggttgccgac gatcgcccag tagatgcgcc
     1021 cgagaaagcc gatcgccagc acgtagccgg cggcgaaatg cgcaaagcgg aggtaaccca
     1081 tcaggaagct gtggctggcc tcgcccggca gcgacggcag cggactgccg atgaagtagc
     1141 cggtcacgcc gagcacgagg atcgccagcg cgttgaccca gtgccagagc cgcaccgggg
     1201 cctcgtagac gtacaccacc tgtggtgcac ggatgacttc aatcgattcg ctcatcttcg
     1261 gtttgccccc cggaagatcg cgtgcgcagc ccgccgttgc ggctgcgcac gccttgcact
     1321 caggcgccgg ccagcagcca gagcccgctc aggcccatcg ccgtgccgat cgcgcgcagc
     1381 caccaggtgc gctgctgcgc gagccgcgtg ccgatggcga tgccggccag atgcagcagg
     1441 ccggtggcga gcaggaagcc cgcggcatag ccatacggcg aggcggcctc cggcagtccg
     1501 gcaccgtgcg caacgccgtg gaacagcgcg aacacgctaa ccagccctgc acctgcggcg
     1561 agcggcagac gcacgagacc ggcgagcagc aggccgagca ccagcaccga aaccccgatg
     1621 ccgggttcga cgaagggcag cgtcaggccg ctcagtgcca gcgttgcccc accggccatg
     1681 gccgtcagga acgccagcgg cagcacgaac ttctgctgcg gcacggccca ggccgaccac
     1741 aggccgacgg cgagcatcgc cagcaggtga tcgaggccgc cgagcgggtg ggcgaggccg
     1801 gcggggaagc cggaagcctc ggcgtggaag ggatgggcgt gagcggccgg gatcacggcc
     1861 gcgagcagga acagcatgag cagcttgcgc atcatttcgg aactcatcgg gactcctcgg
     1921 gactccggat tcgggcggga aggaacgggg gcaggttcac tttagcgaac ccggaccgtg
     1981 ctcagttcct cgccgtccgg tcccatcacg tgcgtcgagc aggcgagaca gggatcgaag
     2041 ctgtgcagcg tgcgcaggat ctcgaccggc tcgtcggcac gcgccaccgg cgtgttcagc
     2101 agtgcggcct cgtaggcgcc gatgttgccc tgcacgtcgc gcgggctggc gttccaggtg
     2161 gtcggcacga cggcctgata gctttcgatg cgcccgtcct cgatgcgcac ccagtggccg
     2221 agcgcgccgc gcggggcttc ggtgaaaccg acgcccttcg ccgacttcgg ccaggtcgac
     2281 gggtcccact tctcgacgtt ggcggtcgat tcgtcaccgg ccttgatggt cgcgaccagc
     2341 ttgtcctgga agtggcgcat ctcggcggcc gcccacgagg cttcgaggcc gcgggcgagc
     2401 gtgcggccga cggtcgagaa cagggcttcg agcggcaggc cgagatcgcg gagtgccttg
     2461 tcgaccggct ccctgaactc ggggcggttc tgcgcgtagc cgatgatgta gcgcgccagc
     2521 ggcccgacct cgaccgcgtg gcccttccag cgcggcgcct tgatccacga gtacttggcg
     2581 ctttcgtcga gggcctcgat gcgggtcttc gtgcctttgg tgttcggccc gagcgtgaag
     2641 tccggctcgg tgacgccctc ccaggggtgc ctgcccttgc cggcgccttc gggatagcga
     2701 taccagctgt gatcgacgaa ctcctgcacc tcggccgggt cgcgcacgtc gacgttctgg
     2761 atgttcctga ggtcgccgcc gatgatcgcg ccgtgcggga gcttgaggtt cgcggccccg
     2821 tagtcgttgg cccgctccgg aatgtcgccg taggacatca cggccttcga ggacaggccg
     2881 ccgccgtacg tccagtcctt gtagaacgag gcgatggcca tcaggtcggg cagatagacc
     2941 tgatcgatga actcgcgcgt gcggtcgatg atgctgctga cgtggttcag ccgctccatg
     3001 ttgatcgcgc cgaccgcgcc ggtcgtgtgc acgttgatcg cgcacggcat gccgccgacc
     3061 agccagttcg gatgcgggtt cttgccgccg tagatcgtgt ggatcttgac gatctcgcgc
     3121 tggaaatcga gggcttcgag gtaatgggcg accgcgagca gatcgacctc ggccggcagc
     3181 cggtaggccg ggttgctcca gtagccgttc ctgaacaggc cgagctggcc gctctcgacg
     3241 aacttcttca gccgggtctg gatgtcgcgg aagtagcccg gactcgacag cggccacgcg
     3301 gagatcttct gcgcgatctc gctggtcgcc ttcggatcgg ccttcagcgc gttgaccggg
     3361 ttgacccagt cgagcgcgtg caggtgatag aagtgcacga ggtggtcgtg cgcctgcagc
     3421 gtcagctgca tgaggttgcg gatcgagttg gcgttctcgg ggatctggat gccgagcgca
     3481 tcctcgaccg cgcgcaccga ggtcagcgcg tgcgtgccgg tgcagacgcc gcagatgcgc
     3541 tcgacgaagg cccaggcatc gcgcggatcg cggcccttga ggatgacctc cagaccgcgc
     3601 cacatcgtgc cggtcgacac ggcattggtg atgacgttgt tcgcgtcgag gttgacctcg
     3661 cagcgcatgt ggccttcgat gcgcgtcacc ggatcgacga cgacgcgcgt gccggcggca
     3721 tcgagccgga aaccgttcgg ggtattgatg acggccatga tcagcggtcc tccctgcgct
     3781 cgtcggtctg cacgtgcttg acgcgcgccc gggtcacggc gctgacggcc gcgtgcgcgg
     3841 cgaccgcggc gccgacgacg ccggcggcgg tggcaccgac gcggtcggcg ttctgttcga
     3901 cgccgaactg ctggatgccg gtcaggcggt catagaacga acccttgtcc cagaagccgt
     3961 cttcggaaca accgatgcag ccgtggccgg actggatcgg gaacgaggtg ccttcgtt
//
LOCUS       DAOQIE010000333         4004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13680_length_4004_cov_7.494809, whole genome shotgun sequence.
ACCESSION   DAOQIE010000333 DAOQIE010000000
VERSION     DAOQIE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13680_length_4004_cov_7.494809"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /gene="prsT"
                     /locus_tag="PK144_16205"
     CDS             <1..2250
                     /gene="prsT"
                     /locus_tag="PK144_16205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNF67317.1"
                     /translation="ARTRLQTLLARQPAPAAHIARLAWTLLGDGELADAQYTAALQAY
                     DAALPLADAASVPQLRLRRGLVRVQLGDDAGARADFEAVAERLPDQPLMHYGLGLLAL
                     RRQQPAEAVTAFERALQRAPEDAGTHEQLARARLALGQREPALADLSGFAARHPEADG
                     VARLLGRLRLSAGDGPGAEAAIQPLLARHPDDAEALEILGMAALLQGRYQEGEAQLRR
                     LVELRPESADAQMRHGLAQLALGEHDAALQSLGAAAGRAPGAQEQARALLLARLASGR
                     HAEALAQAQALSAAHPEAVEPLLYAGAAHEGLQDLPAARADYERALALVPGQAQAAAA
                     LARLDLLDGRPDSARARYQAVLARSPRHVPTLMALARLEWLHGRPEAAIAALETVAGI
                     APEAVAPQLLLARLQAQHGRLADAVTRLDSLHFAHPDDEAVLAHLAAARLQAGDIAET
                     ERLHRDWLARRPDSVAALYGLAAGAAARGDAEALRQALIPALRADREAAPAPALLTRL
                     DALAADPARARAWLAQLQQAVPDHPALLDHAGERLGREQGAAKAAAYYALLRGRQPDE
                     ARWPQREAAWREAAGEGAAAARMLETWLARHPQAVGLRLQLATLYERLDRLEDARAAF
                     VLVLSQRPDEVVALNDLAWLLRDRDPQQALVHARRAALLAPRPEVLETLGEVLIAAGQ
                     PEAAIGVLDTASRGEPSASSIRLNLARAQLASGRVEAARTTLRALLAGAQSFEGRDAA
                     LALLGRAGG"
     gene            2382..2696
                     /locus_tag="PK144_16210"
     CDS             2382..2696
                     /locus_tag="PK144_16210"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNF67318.1"
                     /translation="MTQFSVPSLESLSAPVVKAGKLNVASLEKLTAFNFSLLQSYSDL
                     SLARLKAATDVTDLASLKAYGESSVEVAKELGAKLQADAKAYADLLAGVGAEYKDLVK
                     AA"
     gene            complement(2778..3431)
                     /locus_tag="PK144_16215"
     CDS             complement(2778..3431)
                     /locus_tag="PK144_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNF67319.1"
                     /translation="MSALRFWLLLLVAAVLSALPLIGLPLRWAGTFFHEFSHGLMAAA
                     TGGDIVRIVLRTDGSGLCVFTGGWGVPVAFAGYAGAPLWGLGIFALASGDSQRDDAPI
                     LAGLLGGTVAITALLYARDAVTIVISALLVALLLLPLRRRWRNGRALQAGLEVLGLAV
                     MLDAVQAPLALFGLSGRHDGADLAAHTWLPAPLWAGLWCVLAGACLWLAWRRVRALR"
     gene            complement(3451..>4004)
                     /locus_tag="PK144_16220"
     CDS             complement(3451..>4004)
                     /locus_tag="PK144_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67320.1"
                     /translation="TSGRGVDRRDRSYGFGGGLRVPVAAGWDLKPMFRYGTVVDEDWD
                     HVGSLASASLTSHFRTSFGNGYEFGADNLIGYFQSVGGRVEGLNAGYRLHEVALHNAV
                     RVGGPLEGTFLGSGARWTAWISDTRFLGSELAADNFQRIGIAASTRVRVGERFSENLN
                     LGLHYTQSAGGDHGVELNFGYRF"
BASE COUNT          578 a         1540 c         1417 g          469 t
ORIGIN      
        1 gcgcgcaccc gactccagac cctgctcgcg cgccagccgg cgcccgccgc acacatcgcc
       61 cggctggcct ggacgctgct cggcgacggg gagctggcgg acgcgcagta cacggccgcc
      121 ctgcaggcct atgacgcagc cctgccgctc gccgatgcgg cttcggtgcc gcaactgcgg
      181 ctgcgccgcg gcctcgtgcg cgtgcagctc ggcgacgatg ccggtgcccg cgccgacttc
      241 gaggcggtcg ccgagcgcct gcccgatcag ccgctgatgc actacggcct cggcctgctg
      301 gcgctgcgcc gccagcagcc ggccgaagcg gtcacggcct tcgaacgcgc cctgcagcgc
      361 gcgcccgaag acgccggaac ccatgaacag ctcgcccgcg cccggctcgc gctcggacag
      421 cgcgaaccgg cgctggccga tctgtccggc ttcgccgccc gccatcccga agccgacggc
      481 gttgcccggc tgctcggccg gctgcgcctg tccgccggtg acggcccggg cgccgaggcg
      541 gcgatccagc cgctgctggc acgccatccc gacgacgccg aagcgctgga aatcctcggc
      601 atggccgcgc tgctgcaggg gcgttatcag gagggcgagg cgcagctgcg ccggctggtc
      661 gaactcaggc ccgaatcggc cgatgcgcag atgcgccacg gcctggcgca gctcgccctc
      721 ggcgagcacg acgcagcgct gcagtcactc ggtgccgccg ccgggcgcgc gccgggtgcg
      781 caggagcagg cccgggcgct gctgctcgcc cggctcgcca gcggtcgtca cgccgaagcc
      841 ctggcgcagg cgcaggcgct gtcggccgcg catccggagg cggtcgagcc gctgctctac
      901 gccggcgcgg cgcatgaggg gctgcaggac ctgccggcgg cccgggccga ttacgagcgc
      961 gcgcttgccc tcgtgcccgg ccaggcgcag gccgctgccg cgctggcgcg gctcgacctg
     1021 ctcgacggcc ggcccgattc agcccgtgca cgctatcagg ccgtgctcgc ccgcagcccc
     1081 cggcatgtgc cgacgctgat ggcgctcgcc cggctcgaat ggctgcacgg ccggcccgaa
     1141 gccgcgatcg cggcgctgga gaccgtggcc ggaatcgccc ccgaagccgt ggcgccgcag
     1201 ctgctgctcg cccgcctgca ggcgcagcac ggccggctcg ccgacgccgt cacccgcctc
     1261 gacagcctgc acttcgccca tcccgacgac gaagccgtgc tggcgcatct ggcggcggcg
     1321 cgcctgcagg ccggcgacat cgccgagact gaacgactgc atcgcgactg gctcgcgcgc
     1381 cggccggact cggtggcggc gctgtacgga ctggccgcgg gcgcggcggc ccgcggcgac
     1441 gccgaagcgc tgcgccaggc cctgattccg gccctgcggg ccgatcgcga ggccgcgccg
     1501 gcgccagccc tgctcacacg gctcgacgcg ctcgcggccg atccggcccg ggcccgggcc
     1561 tggctcgcgc agctgcagca ggccgtgccc gatcatccgg ccctgctcga tcacgccggc
     1621 gaacggctcg gccgcgagca gggagctgcg aaggcggcgg cctattacgc gctgctgcgc
     1681 ggccggcagc ccgacgaggc ccgctggccg cagcgcgagg cagcctggcg cgaggcggcc
     1741 ggggaggggg cggccgcggc ccgcatgctg gagacctggc tcgcccggca tccgcaggcc
     1801 gtcgggctgc gcctgcagct cgcgaccctg tacgaacggc tcgaccggct ggaagacgcg
     1861 cgcgccgctt tcgtgctcgt gctgagccag cggcccgatg aggtcgtggc cctcaacgac
     1921 ctcgcctggc tgctgcggga tcgcgacccg cagcaggcgc tcgtccacgc ccgcagggcc
     1981 gcgctgctgg ccccgcggcc ggaggtgctg gagacactcg gcgaagtgct gatcgccgcg
     2041 ggccagccgg aagcggcgat cggcgtactg gacacggcga gccgcggcga gccgtcggcc
     2101 agctcgatcc ggctgaacct cgcccgggcg cagctcgcca gcggccgcgt cgaggcggcc
     2161 cgcacgacgc tgcgcgcgct gctcgccggt gcgcagtcct tcgaaggacg cgatgccgcg
     2221 ctcgcgctgc tcggcagggc cggcggctga atcacgggga aaggaacagg gtcgggacag
     2281 aaggctcaaa aggatgttgc actgcagcat cgcatggata taatggcacc catgggttca
     2341 ccggaacccg accgccccgc cacccaaacg aggatgcagc catgacccag ttctccgttc
     2401 cgtcgctcga gtccctgtcc gcccccgtcg tcaaggccgg caagctgaac gtcgccagcc
     2461 tcgaaaagct gaccgccttc aacttcagcc tgctgcagag ctacagcgac ctgagcctgg
     2521 cgcgcctgaa ggccgccacc gacgtgaccg atctggccag cctgaaggcc tacggcgagt
     2581 cctcggtcga agtcgcgaag gagctgggtg ccaagctgca ggccgacgcc aaggcctatg
     2641 ccgacctgct cgccggcgtc ggcgccgagt acaaggatct ggtcaaggcc gcctgatcgc
     2701 cggcgccccg accttcctga aaaccgcccg caccgtcggg cggtttttgt tttccggtcc
     2761 gccggcccgg ccatcgttca gcgcagcgcg cgcacccgcc gccaggcgag ccacaggcag
     2821 gcccccgcca gtacgcacca gagcccggcc cacagcggcg ccggcagcca ggtgtgcgcg
     2881 gccagatcgg caccgtcatg ccggccggac agcccgaaca gcgcgagcgg cgcctgcacg
     2941 gcatcgagca tcacggccag ccccaggact tcgagcccgg cctgcagcgc ccgcccgttg
     3001 cgccagcgcc ggcgcagcgg cagcagcaac agcgcgacca gcagcgccga gatcacgatc
     3061 gtcaccgcat cgcgcgcata cagcagcgcc gtgatcgcga ccgtgccgcc gagcaggccg
     3121 gcgaggatcg gcgcatcgtc gcgctgcgaa tccccgctcg ccagcgcgaa gatgccgagc
     3181 ccccacagcg gcgcccctgc gtagcctgcg aaagccaccg gcacgcccca gccgccggtg
     3241 aacacgcaca gtccggagcc gtcggtgcgc agcacgatgc gcacgatgtc gccaccggtc
     3301 gccgcggcca tcagcccgtg gctgaactcg tggaagaagg tgccggccca gcgcagcggc
     3361 agcccgatca gcggcagcgc cgacagcacg gccgccacga gcagcagcag ccagaaccgc
     3421 agtgcgctca tcggcacagc ctcccgggtc tcagaagcgg tagccgaagt tcagctcgac
     3481 accgtgatcg ccgccggccg actgcgtgta gtgcaggccg aggttcaggt tctcggagaa
     3541 gcgttcaccg acgcgcacgc gcgtgctcgc ggcgatgccg atgcgctgga agttgtcggc
     3601 cgcgagctcc gaaccgagga agcgcgtgtc cgagatccac gccgtccagc gcgcgccgct
     3661 gccgaggaag gtgccctcca gcggcccgcc gacgcgcacg gcgttgtgca gcgcgacttc
     3721 gtgcaggcgg tagccggcgt tcagtccttc gacacgaccg ccgaccgact ggaagtagcc
     3781 gatcaggttg tcggcgccga actcgtaacc attgccgaag gacgtccgga agtggctggt
     3841 cagcgaggcc gaggccagcg agccgacgtg gtcccagtcc tcgtcgacga cggtgccgta
     3901 acggaacatc ggtttcaggt cccagccggc ggcgaccggt acgcgcaggc caccgccgaa
     3961 gccgtagctg cggtcacggc gatcgacgcc ccggccgctg gtcg
//
LOCUS       DAOQIE010000334         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13693_length_4003_cov_6.780648, whole genome shotgun sequence.
ACCESSION   DAOQIE010000334 DAOQIE010000000
VERSION     DAOQIE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13693_length_4003_cov_6.780648"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1468
                     /locus_tag="PK144_16225"
     CDS             224..1468
                     /locus_tag="PK144_16225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67321.1"
                     /translation="MDSQALPAVLPSAERKVLKAQLRRAERSHRLRSLALIAPLLVFL
                     IASFVLPIGTLLERAFDDSDLPKTLPATLAVLEKWDGQALPGEAAYAALALDLRELRE
                     SGRLGIPAKRLGYEISGFRSLLNKTVRKLPESAPYKDALVAANPEWGELPYWQAIRNA
                     AGPHTARYLLAALDREHNAAGEIVVNPESAFIDVFLRTFWIAGVVTAWCLLLGYPVAY
                     WLSILPPGKANLLMILVLLPFWTSLLVRTAAWIVLLQSGGLLNGLLMALGFTDQPLEL
                     VFNRFGVIVAMTHILLPFMILPLYSVMKGIPPYYVRAAVSLGAHPFLAFWKVYVPQTI
                     AGVGAGCLLTYILALGYYITPALVGGPADQMVSYFVAFYTNKTINWGMASALGALLLA
                     ATLVLYAVYGRLVAAGNPQPRR"
     gene            1502..2329
                     /locus_tag="PK144_16230"
     CDS             1502..2329
                     /locus_tag="PK144_16230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67322.1"
                     /translation="MNLPLSTWPERLWHHAFRLICWAILIFLIVPILVIVPLSFNSSS
                     FLSYPLSGLSMRWYTEFFGSNEWMLALKNSFIVAPLATLLATAAGTLAAVGLARGEFR
                     GKGLIMGILISPMVVPVVIVAVGLYFFYAEHGLLNTYTGLVLAHAVLGVPFVVITVTA
                     TLQGYNTNLSRAAASLGAPPLTVFRRIVLPLIAPGVISGALFAFATSFDEVVVTLFLA
                     SPTQRTLPMQMFGGIRENISPTIAAVATLLITLSVLMLLTMEWLRRRSEKLRGSGNT"
     gene            2593..>4003
                     /locus_tag="PK144_16235"
     CDS             2593..>4003
                     /locus_tag="PK144_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HNF67323.1"
                     /translation="MNTANTENTGNTENTAKRPVLKGLSEIFTYFRTNDVPIWFVSPT
                     PYNVLGLDRWVRRFEYVCYFDSFDGWHPKIRIPRETGPREFRSIEDVNNYLLSHKEIA
                     DRMRAAGGGKLVLVMFDEETEALAKELGVEIALPPAELRKRLDSKIETTRIGNEAGVF
                     SVPNVLGRADDYDALLALAATVGLGSDLVVQTPYGDSGRTTFFIASRADWDEFADKII
                     GEDLKVMKRINHLPGTIEGIATRHGTLVGPLMVDITGFEELTPYKGGWCGNDVAPKVL
                     PEARARVHEMTRRLGDRLYREGYRGTFCMDWLLDTDTGEVWLGELNPRVSGASPMTNL
                     ITSTYGGVPMFLFHLLEFMDVDYEIDLEAVQRRWEEFDTWSQLILKHTGDEVEIITQA
                     PTSGIWKMNADGAIHFERRDIDWGGVTGEDEAFYLRVYNAGDYRYHGADMGILVSRGR
                     MQTDDRELFPRAKQWAAGIL"
BASE COUNT          629 a         1400 c         1264 g          710 t
ORIGIN      
        1 cccctctccc ccggcccctc tcccgcaagg ggagagggga ggtcgaagcc ggagcgtcgg
       61 tgtggatttc ctgccgcctg cggaaactgc agggttctgc agatacccgt ggcgacggga
      121 gacaggctga gccgcgcgtc gacgcgatga agtgcttcgc agtccgcctg cgacgtcgtc
      181 gaagtttccg atgtttccac accctgtctg acggagagcc gtgatggact cccaagccct
      241 gcctgccgtc ctgccgagcg cggaacgcaa ggtcctgaaa gcgcagctgc gccgtgccga
      301 gcgttcgcac cggctgcgct cgctggcgct gatcgcgccg ctgctggtgt tcctgatcgc
      361 gagcttcgtg ctgccgatcg gcacgctgct cgaacgcgcc ttcgatgaca gcgacctgcc
      421 gaagacgctg ccggcgacgc tggcggtgct ggagaaatgg gacggccagg cgctgcccgg
      481 cgaagcggcc tacgcggcgc tggcgctcga cctgcgcgag ttgcgcgaga gcggccggct
      541 cggcatcccg gccaagcgcc tcggctacga gatctcgggc ttccgcagcc tgctgaacaa
      601 gaccgtgcgc aagctccccg agagcgcccc gtacaaggac gcgctggtcg cggcgaatcc
      661 ggagtggggc gagctgccgt actggcaggc gatccgcaat gccgccggtc cgcacacggc
      721 gcgctacctg ctcgccgcgc tcgatcgcga gcacaacgcc gccggcgaga tcgtcgtcaa
      781 ccccgagtcg gccttcatcg acgtgttcct gcgcaccttc tggatcgccg gcgtggtcac
      841 ggcctggtgc ctgctgctcg gctatccggt cgcctactgg ctgtcgatcc tgccgcccgg
      901 caaggccaac ctgctgatga tcctcgtgct gctgccgttc tggacctcgc tgctggtgcg
      961 caccgcggcc tggatcgtgc tgctgcagtc cggcggactg ctcaacggcc tgctgatggc
     1021 gctcggcttc accgatcagc cgctggagct ggtgttcaac cgcttcggcg tcatcgtcgc
     1081 gatgacccac atcctgctgc cgttcatgat cctgccgctg tactcggtca tgaagggcat
     1141 cccgccgtac tacgtgcggg cagcggtgtc gctcggcgcg catccgttcc tggccttctg
     1201 gaaggtctac gtgccgcaga ccatcgccgg cgtcggcgcc ggctgcctgc tcacctacat
     1261 cctcgcgctc ggctactaca tcacgccggc gctggtcggc ggcccggcgg accagatggt
     1321 cagctacttc gtcgccttct acacgaacaa gaccatcaac tggggcatgg cctcggcgct
     1381 cggcgcgctg ctgctcgccg cgacgctggt cctgtacgcg gtctacggcc ggctggtcgc
     1441 ggccggcaac ccgcaacccc gtcgctgagc ccgcgtccgc gctgcttccg gagccgccgc
     1501 catgaacctg ccgctgtcca cctggcccga acgcctctgg catcacgcct tccggctgat
     1561 ctgctgggcg atcctgatct tcctgatcgt gccgatcctc gtcatcgtgc cgctgtcgtt
     1621 caattcgagt tcgttcctga gctacccgct gtccggcctg tcgatgcgct ggtacacgga
     1681 gttcttcggc tcgaacgaat ggatgctggc gctgaagaac agcttcatcg tcgcgccgct
     1741 ggcgacgctg ctcgccaccg ctgccggcac gctggccgcc gtcggccttg cccgcggcga
     1801 gttccgcggc aagggcctga tcatgggcat cctgatctcg ccgatggtcg tgccggtggt
     1861 catcgtcgcg gtcggcctgt acttcttcta tgccgagcac gggctgctga acacctacac
     1921 cggcctcgtg ctcgcgcatg cggtgctcgg tgtgcccttc gtcgtcatca ccgtcaccgc
     1981 gacgctgcag ggctacaaca ccaacctgtc gcgtgccgcc gccagcctcg gcgcgccgcc
     2041 gctgaccgtg ttccggcgca tcgtgctgcc gctgatcgcc cccggcgtca tttccggtgc
     2101 gctgttcgcg ttcgcgacct cgttcgatga agtggtcgtg acgctgttcc tcgccagccc
     2161 cacgcagcgc acgctgccga tgcagatgtt cggcggcatc cgcgagaaca tcagcccgac
     2221 catcgccgcg gtcgccacgc tgctgatcac gctgtcggtg ctgatgctgc tgaccatgga
     2281 atggctgcgc cggcgcagcg agaagctgcg cggcagcggc aatacctgat accgatcccg
     2341 ctagacacgt tgtttttccc cctctcccca acccctcccc cgcgaggggg gaggggctga
     2401 aaacacagcg tgttcagcgg tgatcggcat gaagcccaaa aacggccctg aatcatgttc
     2461 gaccgactgc tggccgcgct ctgtcgcggc tggcgggccg tgtgcgccct gttcgccacg
     2521 ccgcccgtca ctgaaacgaa tgttccggct cccatccgaa ttccaaccga tcgcccgtcc
     2581 ggggaaatcg ccatgaacac agcaaacaca gaaaacaccg gtaataccga aaacaccgcc
     2641 aagcggcccg tgctgaaggg cctgtccgaa atcttcacgt acttccgcac caatgacgtg
     2701 ccgatctggt tcgtgtcgcc gacgccgtac aacgtgctcg gactcgaccg ctgggtgcgc
     2761 cgcttcgaat acgtctgcta tttcgattcc ttcgacggct ggcacccgaa gatccgcatt
     2821 ccgcgcgaaa ccggcccgcg cgagttccgt tcgatcgagg acgtgaacaa ttacctgctg
     2881 tcgcacaagg agattgccga ccgcatgcgt gccgccggtg gcggcaagct ggtgctggtg
     2941 atgttcgatg aggaaaccga ggcgctcgcg aaggaactcg gcgtcgagat cgcgctgccg
     3001 ccggccgaac tgcgcaagcg cctggattcc aagatcgaga ccacgcgcat cggcaacgag
     3061 gccggcgtgt tcagcgtgcc gaacgtgctc ggtcgtgccg atgattacga cgcgctgctc
     3121 gcgctcgccg ccaccgtcgg tctcggcagt gatctggtcg tgcagacgcc ttatggcgac
     3181 tccggccgca cgaccttctt catcgccagc cgcgccgact gggacgaatt cgccgacaag
     3241 atcatcggcg aggacctgaa ggtcatgaag cgcatcaacc acctgcccgg cacgatcgaa
     3301 ggcatcgcca cgcgccacgg cacgctggtc gggccgctga tggtcgacat caccggcttc
     3361 gaggaactca cgccgtacaa gggcggctgg tgcggcaatg acgtggcgcc gaaggtgctg
     3421 cccgaagcgc gcgcccgcgt gcacgagatg acgcgccggc tcggcgaccg gctgtatcgc
     3481 gagggctacc gcggcacctt ctgcatggac tggctgctcg acaccgacac cggcgaggtc
     3541 tggctcggcg agctgaatcc gcgcgtctcg ggcgccagcc cgatgacgaa tctgatcacg
     3601 tcgacgtatg gcggcgtgcc gatgttcctg tttcacctgc tggaattcat ggatgtcgat
     3661 tacgagatcg acctcgaagc cgtgcagcgg cgctgggagg agttcgacac ctggagccag
     3721 ctgatcctca agcacaccgg cgatgaggtg gaaatcatca cgcaggcccc gacctcgggc
     3781 atctggaaga tgaacgccga cggcgccatc cacttcgagc gccgcgacat cgactggggc
     3841 ggcgtcaccg gcgaggacga agccttctat ctgcgtgtct acaacgctgg cgattaccgc
     3901 taccacggcg ccgacatggg catcctggtc agccgcggcc gcatgcagac cgacgaccgc
     3961 gaactgttcc cgcgcgcgaa gcaatgggcg gccggcatcc tca
//
LOCUS       DAOQIE010000335         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13789_length_3986_cov_7.765200, whole genome shotgun sequence.
ACCESSION   DAOQIE010000335 DAOQIE010000000
VERSION     DAOQIE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13789_length_3986_cov_7.765200"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(708..2384)
                     /locus_tag="PK144_16240"
     CDS             complement(708..2384)
                     /locus_tag="PK144_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNF67324.1"
                     /translation="MWSQVYDPLNNAWLSTAAAAIPVIVLLGALGFFHIKAHIAALMG
                     LAASLLVAIAVFGMPASTALSSATFGAANGLMPIGWIILNVIFLYQLTERKGQFAILR
                     ESITGVTNDRRLQLLLIAFSFGAFFEGAGGFGTPVAVTGAMLIGLGFSPLAASGLSLI
                     ANTAPVAYGALGTPIITLAKVTGLDVMQLSAMVGRQLPLFSLIVPFWLVWAFCGFRNM
                     IKVWPAVLVAGLFFAIPQFVVSNYFGPELVDVLAAIISLAALALFLKFVWHPKEVWTS
                     TSGKAEDNVEHVGGHHGYTGGEVLRAWLPWIILSVLVLIWGMPEVKKFWNGVWAPVFE
                     WPGLHNMVQKVPPIVAHPEIEGAKYTFGILSATGTGILIAAVISGFIMGYSPLGMLKV
                     YWETLKTLKYSLITIVAMLALGYVTRYSGTDITLGLAFAQTGVFYPFFGTLLGWLGVA
                     LTGSDTAANVLFGGLQKASAQQLGLSPVLMAAANSAGGVMGKMIDAQSIVVASTATRW
                     YGHEGDILRYVFFHSIALACLVGILVTLMAYVPPFTSLVIWPATGGVPVH"
     gene            3045..3800
                     /locus_tag="PK144_16245"
     CDS             3045..3800
                     /locus_tag="PK144_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNF67325.1"
                     /translation="MKPAKISDVITHQIEALILEGGLKPGDRLPPERELAARFEVSRP
                     SLREAIKKLETRGLVETRRGGGTYVSSLLDASFANPLMGLLHNHPEALYDLVEMRHSL
                     ESVAAYYAAVRGTAEDKAALRTRFEAMIAVHGKGGGDPQTDARLDTEYHIAIAEAAHN
                     VVLLYIMRGLFNLLHSGITTSLARLYTRSGNHEAICQQHTDLFEAIMAGDGERARKAA
                     SDHLLFVKRSLAEIDEEEKRIERARRRLHGLTD"
BASE COUNT          729 a         1303 c         1290 g          664 t
ORIGIN      
        1 ctcctccttt ggtggtcttg tttgaagccg ggctccccgc ccggctcttt ttttgcgcgt
       61 cattctgtca gttgcctgac gaaagcccgc ggcgctccgt ctctgccgcg tggccggccg
      121 cagcccggat accggcgtgc agtccgcacg gtggacggct gggcgacacc ggctctcatg
      181 actttatgtt cacttgacgg caacagattt tgtttatcga acccgttgac gatccggcgg
      241 atgatccata tgatgcaacg caacatctgc ggatgttctc ctcctccttt ggtggtcttg
      301 tttgaagccg ggctccccgc ccggctcttt ttttgtccgt cattcaggtc cttgcctgac
      361 gccagcctgc ggtgatccgc ctctgccgcg cggccggccg cgggccggat gccggcgccg
      421 ggacgctctc ccgtgccgga gctcgcctgc ctctccgacg caggctcgtc gtcctgaaat
      481 cggtctgatg actggcatct gacatcggtg tttgaacggc accgcattaa atgatggtat
      541 aaaaatttat ctatacatcc gaatgagtgc atgtcttccg gcccgatgcg cgcccgccga
      601 ctgccgccct gctccgccgg agcgcccgac atgggtgccc ggaaaagcag aaggggcctt
      661 gcggcccctt cgttcggagt gtcgggaatg tcgtcaggct gcgccgctca gtgcaccggc
      721 acgccgccgg tggccggcca gatcaccagg ctggtgaacg gcggcacgta ggccatcagc
      781 gtcacgagga tgccgaccag gcaggcgagc gcgatcgagt ggaagaacac gtagcgcagg
      841 atgtcgcctt cgtggccgta ccagcgggtc gcggtcgagg cgacgacgat ggactgcgcg
      901 tcgatcatct tgcccatgac gccgccggcg ctgttcgccg cggccatcag caccggcgac
      961 aggccgagct gctgcgccga agccttctgc aggccgccga acagcacgtt ggcggcggta
     1021 tccgaaccgg tcagcgccac gccgagccag ccgagcagcg tgccgaagaa ggggtagaac
     1081 acgccggtct gcgcaaaggc gaggccgagg gtgatgtcgg tgccggagta gcgggtcacg
     1141 tagccgagcg cgagcatcgc gacgatcgtg atcagcgagt acttcagggt cttcagggtt
     1201 tcccagtaga ccttgagcat gccgagcggg ctgtagccca tgatgaagcc cgagatgacg
     1261 gcggcgatga ggatgccggt accggtcgcc gacaggatgc cgaaggtgta cttggcgcct
     1321 tcgatctccg ggtgcgcaac gatcggcggc accttctgga ccatgttgtg caggcccggc
     1381 cactcgaaca ccggcgccca gacgccgttc cagaatttct tgacctcggg catgccccag
     1441 atcagcacca gcaccgacag gatgatccac ggcagccagg cgcgcagcac ctcgccgccg
     1501 gtgtaaccgt ggtgaccgcc gacgtgctcg acgttgtcct cggccttgcc gctggtcgag
     1561 gtccagactt ccttcgggtg ccacacgaac ttcaggaaca gcgccagcgc ggccagcgag
     1621 atgatcgcgg cgagcacgtc gaccagctcg ggaccgaagt agttggagac gacgaactgc
     1681 gggatcgcga agaacaggcc cgcaaccagc accgccggcc agaccttgat catgttgcgg
     1741 aagccgcaga aggcccagac cagccagaac ggcacgatca gcgagaacag cggcagctgg
     1801 cgaccgacca tcgccgagag ctgcatgaca tcgaggccgg tgaccttcgc gagcgtgatg
     1861 atcggcgtgc cgagcgcgcc ataggcgacc ggcgcggtgt tggcgatcag cgacaggccc
     1921 gaggcggcga gcggcgagaa gccgaggccg atcagcatcg cgccggtgac ggcgaccggc
     1981 gtgccgaagc cgccggcacc ctcgaagaac gcgccgaagc tgaacgcgat cagcagcagc
     2041 tgcagccggc ggtcgttggt gacgccggtg atgctctcgc gcaggatcgc gaactgcccc
     2101 ttacgctcgg tcagctgata cagaaagatg acgttcagga tgatccagcc gatcggcatc
     2161 aggccgttgg cggcgccgaa ggtcgccgag ctgagcgcgg tcgaggccgg catgccgaac
     2221 acggcgatcg ccaccagcag cgaggccgcc aggcccatca gcgccgcgat gtgcgccttg
     2281 atatggaaga acccgagcgc gccgagcagc acgatgacgg gaatcgccgc ggctgccgtc
     2341 gacagccagg cgttgttgag cgggtcgtag acttgggacc acatggttgt ttgttccccc
     2401 ctccgggaaa agcgcgcgca cgcgccgatc agcgccatcc gcaggcggac ggacggcgcc
     2461 ggtcactgac agctcacgtt cggacgaagg ccccgcggtc aggggaccgc gaggccggac
     2521 agatcgcgga tcgaccggct ccttcgcaag gtttccttac aggtgcgacg gagcaattgg
     2581 tcaactgatc tgaccactat tggaccggat atgaaatcgg cccgcaagcc tgttgcacgt
     2641 gccggtatga aattttctca gcttcgcccc ggtttcaggg catccggcct gaccaatatg
     2701 accggaaatt cgcacacctg taagaaacgg acccccaacc gattgcgcat cgttgtcacg
     2761 cttccgcatt ccggtaaatc atcggaatca ctgacaactt gccgccggtc tgccggcaga
     2821 tgccgcccgg ttgcggaggt ctgtcgtggc gcacccggtc cgcaagcgtg gcgatatttc
     2881 atcgggaaca agccaggcag tcattgtcga ggtcatgcgg cctgaccaat cagacccggg
     2941 acggtgcgga tgcaccaaaa tgtgccgtct cgacaggctc acagcgcgac tgttaggctt
     3001 aattggtcat accggatttc cgtaccccta tcaggacagc gcccgtgaag ccggccaaga
     3061 tttccgacgt catcacccat cagattgaag ccctgatcct cgaaggcggg ctcaagcccg
     3121 gcgatcgcct gccgcccgag cgcgagctgg cagcgcgctt cgaggtgtcg cgcccgtcgc
     3181 tgcgcgaagc gatcaagaaa ctcgaaaccc gcggtctggt cgagacgcgc cgcggcggcg
     3241 gcacgtatgt atcgagcctg ctcgatgcct cgttcgcgaa cccgctgatg ggcctgctgc
     3301 acaaccaccc cgaggcgctc tacgacctgg tcgagatgcg tcactcgctg gaatcggtcg
     3361 ccgcctatta cgcggccgtg cgcggcaccg ccgaggacaa ggcggcgctg cgcacgcgct
     3421 tcgaggcgat gatcgccgtg catggcaagg gcggcggcga tccgcagacc gacgcccggc
     3481 tcgacaccga gtaccacatc gccatcgccg aggcggcaca caacgtcgtg ctgctctaca
     3541 tcatgcgcgg cctgttcaac ctgctgcaca gcggcatcac gacctcgctg gcgcgcctgt
     3601 acacgcgctc cggcaaccac gaggcgatct gccagcagca caccgacctg ttcgaggcga
     3661 tcatggccgg cgacggcgaa cgcgcgcgca aggccgcgag cgatcacctg ctgttcgtca
     3721 agcgctcgct cgccgagatc gacgaggaag aaaagcgcat cgagcgcgca cgccggcggc
     3781 tgcacgggct gacggactga tagcggtcac ggctgaggcc gctgtctttt cggcccctcg
     3841 ccccttacgg gggaggggtt ggggagtttg cagctcgcag gatgtgccga cgcagaaggg
     3901 gcatcgttcg cagcatccga gcagcgatta cccgtacctt ccgacgtttt acgcgaacga
     3961 tgcgcttcgt tcctcagcgc atccta
//
LOCUS       DAOQIE010000336         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13810_length_3983_cov_9.527749, whole genome shotgun sequence.
ACCESSION   DAOQIE010000336 DAOQIE010000000
VERSION     DAOQIE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13810_length_3983_cov_9.527749"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..231)
                     /locus_tag="PK144_16250"
     tRNA            complement(156..231)
                     /locus_tag="PK144_16250"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Gly,seq:gcc)
     gene            complement(284..838)
                     /gene="pgsA"
                     /locus_tag="PK144_16255"
     CDS             complement(284..838)
                     /gene="pgsA"
                     /locus_tag="PK144_16255"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNF67326.1"
                     /translation="MHLTLPTWLTLARIAMVPVFVAAFFLPVESANVICTALFAAAAI
                     TDWLDGWLARRLGQTSDFGAFLDPVADKLMVAAALVLLVEADPTPWIGVPAVVIIGRE
                     IAISALREWMAQIGSRATVAVSAVGKVKTALQMLALLLLLYHEPLWGLPTRTLGIVAL
                     VIAAVLTLWSMLVYLAAAWRVLNK"
     gene            complement(853..2688)
                     /gene="uvrC"
                     /locus_tag="PK144_16260"
     CDS             complement(853..2688)
                     /gene="uvrC"
                     /locus_tag="PK144_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNF67327.1"
                     /translation="MTQPSAPAPFDARAFLRTLTPLPGVYRMLDVEGQVLYVGKARNL
                     KRRVASYFRKTVDSAKTRALVAQIAGVEVTVTHTEAEALLLEHTLIQRFRPRYNILLR
                     DDKSYPYLLLTAHPYPRLLIHRGPRRAAGRYFGPYPSATAVRETLTLLQRVFRLRQCE
                     DGFFAHRSRPCLQYQIRRCSGPCTEMIGRAEYAADVADVVAFLEGRSGELIDALVARM
                     EVAAGHWRYEEAAQLRDRIAMLRQVQERQYVSAAEGGDLDVVAVAIEGGAACVQVFFF
                     REGRSLGNKPFYPRMPEEQTPGQVLSAFLVQYYLNHPPPAEILLSHEPDEAAVVAEAL
                     GERAGRKVQLSMRVRGERARWLEMANRNAALALAADIASRAGVRRRFEALQDALGLDA
                     AIERMECFDISHTQGEATVASCVVFDGEGPRKSDYRRYNIEGITPGDDYAAMHQALTR
                     RYTRLRDEDAPLPDLLFIDGGRGQLAQAEQVLAELGIDGVHLIGIAKGPDRRPGLEVL
                     HLSGQDRPLTLPAESAALHLVQQIRDEAHRFAITGHRQRRAQARRTSTLEAIPGIGPR
                     RRQLLLKQFGGLQQLARAGVEDIAGVAGISKALAQRIYDAFHPDG"
     gene            2996..3793
                     /locus_tag="PK144_16265"
     CDS             2996..3793
                     /locus_tag="PK144_16265"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67328.1"
                     /translation="MIFKDLRSSGPLLAGAFAVLLGLSAPAADASVSYSFTTTTGNAA
                     AQSYLAPGGTPVTLSAWSNGGTSGTMVQGSLSFWGSSGVGVNNGFGSSTDPSEGVSPE
                     HAIDNNGLTDGVLLNFGVGNLFTMNSFSIGWFDSDADIVVLAYTGAGTPALGSSTLSG
                     LLSSGWSGKKYSDVNSLGTNVAVNTALSLSSQYWLVLAYNSAFAGLSSGLGSSYGAGN
                     DAFKFNGVTGNLTSTSSNISVPLPATTALLGVGALAFWRRRYQRQNS"
BASE COUNT          686 a         1271 c         1365 g          661 t
ORIGIN      
        1 agggtcggaa tcgaaccggc gtccacggct ttgcaggccg ctgcatgacc actctgccac
       61 ctagccttcg gcaatcaggt aagggctgca ttgccattac cgactgatcc gaaaatcaaa
      121 aggccccgaa ggaatcgagg cctctgatcg agaactggag cgggaaacga gactcgaact
      181 cgcgacccta accttggcaa ggttatgctc taccaactga gctattcccg ctcgacgtgg
      241 gggcgcatta taggccgcga atccggatcg tcaagcgccg gattcattta ttcagcacgc
      301 gccaggcggc ggccagatac accagcatcg accacagcgt cagcaccgcc gcgatcacca
      361 gcgcaacgat gccgagcgtg cgcgtcggca gcccccacag cggttcgtgg tacagcagca
      421 gcagcagtgc cagcatctgc agcgccgtct tgaccttgcc gaccgccgac accgccaccg
      481 tcgcgcggct gccgatctgc gccatccact cgcgcagcgc cgagatcgcg atctcgcggc
      541 cgatgatgac cacggccggc acgccgatcc acggcgtcgg atcggcctcg accagcagca
      601 ccagcgccgc ggccaccatc agcttgtcgg cgaccggatc gaggaaggcg ccgaagtccg
      661 aagtctggcc gaggcggcgc gcaagccagc cgtcgagcca gtcggtgatg gcggcggcgg
      721 caaacagcgc cgtgcagatc acgttcgccg attcgaccgg caggaagaag gcggcgacga
      781 acaccgggac catcgcgatg cgggcgaggg tcagccaggt cggcagggtc aggtgcatgg
      841 ggatcctggg attcagccgt ccgggtggaa ggcgtcgtag atgcgctggg cgagcgcctt
      901 gctgatgccg gcgacgccgg cgatgtcctc gacgccggca cgggcgagct gctgcagacc
      961 gccgaactgc ttgagcagca gctgacgccg gcgcggaccg atgccgggga tcgcttcgag
     1021 ggtcgaggtc cggcgcgcct gcgcccggcg ctgccggtgg ccggtgatcg cgaagcgatg
     1081 cgcttcgtcg cggatctgct gcacgaggtg cagcgcggcg gattcggccg gcagtgtaag
     1141 agggcggtcc tgaccggaca agtgaagcac ctccagcccc ggccggcggt ccggaccctt
     1201 ggcgatgccg atgaggtgca cgccgtcgat gccgagttcg gccagcacct gctcggcctg
     1261 cgcgagctgg ccgcggccgc cgtcgatgaa caggagatcg ggcagcggcg catcctcgtc
     1321 gcgcaggcgc gtgtagcggc gggtcagcgc ctgatgcatc gccgcgtagt cgtcgccggg
     1381 cgtgatgccc tcgatgttgt agcgccggta gtcggacttg cgcgggcctt cgccatcgaa
     1441 caccacgcag gaggcgacgg tcgcctcgcc ctgggtgtgg ctgatgtcga agcactccat
     1501 gcgctcgatc gcggcatcga ggccgagcgc atcctgcagc gcctcgaagc gccggcgcac
     1561 gccggcgcgg ctggcgatgt cggcggcgag cgcgagggcc gcgttgcggt ttgccatctc
     1621 cagccagcgc gcgcgctcgc cgcgcacgcg catcgacagc tgcaccttgc gcccggcacg
     1681 ctcgccgagc gcctcggcga cgacggcggc ctcgtccggt tcgtgcgaca gcaggatctc
     1741 ggccggcggc ggatggttca ggtagtactg cacgaggaag gccgacagca cctgccccgg
     1801 tgtctgttcc tcgggcatgc gcgggtagaa cggcttgttg ccgaggctgc ggccctcgcg
     1861 gaagaagaac acctgcacgc aggccgcgcc gccctcgatc gcgaccgcga ccacgtcgag
     1921 atcgccgcct tcggccgccg acacgtactg gcgctcctgc acctgacgca gcatcgcgat
     1981 gcgatcgcga agctgcgcgg cttcctcgta gcgccagtgc ccggccgcga cctccatgcg
     2041 cgcgaccagc gcatcgatca gttcgccgct gcggccttcg aggaaggcca ccacgtcggc
     2101 gacatcggcg gcgtattcgg cgcggccgat catttcggtg cacgggccgg agcagcgccg
     2161 gatctggtac tgcaggcacg ggcggctgcg gtgcgcgaag aagccgtcct cgcactggcg
     2221 cagccggaac acacgctgca gcagcgtcag cgtctcgcgc accgcggtcg ccgacggata
     2281 cggcccgaag tagcgcccgg ccgcgcgccg cggaccgcgg tggatcagca gccgcggata
     2341 cgggtgtgcg gtgagcagca ggtacggata gctcttgtcg tcgcgcagca ggatgttgta
     2401 gcgcggccgg aagcgctgga tcagcgtgtg ttcgagcagc agcgcctcgg cttccgtgtg
     2461 cgtgaccgtg acctcgacgc cggcgatctg cgcgaccagc gcgcgggtct tggccgagtc
     2521 gacggtcttg cggaagtagc tcgccacgcg gcgcttcagg ttgcgcgcct tgccgacgta
     2581 cagcacctga ccctcgacat cgagcatgcg gtagacgccg ggcagcgggg tcagcgtgcg
     2641 caggaaggcg cgggcatcga acggagcggg ggcggagggc tgggtcacgg cgtcagggct
     2701 ttttacacgg aattatggca aatcgtgaca aatgtctcat aactgcaggc acaacaaatg
     2761 cttaacatga ttggcttgtg atgtgcatat aggtgctggt cagccagaca agacaggttg
     2821 gtcgggtagt cgcagcccgc cggccagtgc cgcggcgcag aagccggtcg gtcgaacaag
     2881 gattcacacg gaagcgacga tgttttgagg ggatgcgttc cctcaccatg atggtacccg
     2941 tccctttctg ccccgccgcc cacgttctgc acgaacttcg cggaggggtt tcatcatgat
     3001 tttcaaagac ctgcggtcca gcgggccctt gcttgcgggg gcgttcgcgg tactgctcgg
     3061 cctgagtgcg ccggctgcgg atgcgtcggt cagctattcg ttcacgacca cgaccggcaa
     3121 tgccgcggct cagtcctacc tcgcgcccgg ggggactccg gtgaccttgt cggcctggtc
     3181 aaacggtggt accagcggca cgatggtaca gggcagcctg tccttctggg gaagttccgg
     3241 tgtcggtgtc aacaatggct ttggcagcag caccgatccg agtgaaggcg tcagccccga
     3301 gcacgcgatc gacaacaatg gtctgacgga cggtgtgctg ctcaacttcg gtgtcggcaa
     3361 cctgttcacg atgaacagtt tttcgatcgg ctggttcgat agcgatgccg acatcgtggt
     3421 tctggcctac accggtgccg ggacgcctgc gctgggcagt tcgaccctga gcggcctgct
     3481 gagcagcggc tggagcggca agaaatacag cgatgtcaat agcctcggca ccaatgtggc
     3541 tgtcaacacg gcgttgtcgc tcagttccca gtactggctg gtgctggcct acaactctgc
     3601 tttcgctggt ctgagttctg ggctgggatc ctcgtacggg gccggaaatg acgccttcaa
     3661 attcaatggg gtcaccggta atctgaccag cacatccagc aacatcagcg taccgctgcc
     3721 ggcgacaacc gcgctgctcg gggttggcgc gctggccttc tggcggcgac gttatcaaag
     3781 gcagaacagc tgatacggat ccagcggccc gcaagcgata tcggctggcg tttgggtacg
     3841 gcacgaagaa ggctggattg cagtcgatgt ttgccagaca ccgcccttcg gggcggtttt
     3901 ttttatgggg cccggttccg gagttcaaga acggtgcatt cgcaaaatgc aaatcctgac
     3961 ggggaatttg cagaatgcac gtc
//
LOCUS       DAOQIE010000337         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_13828_length_3980_cov_5.666497, whole genome shotgun sequence.
ACCESSION   DAOQIE010000337 DAOQIE010000000
VERSION     DAOQIE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13828_length_3980_cov_5.666497"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PK144_16270"
     CDS             <1..1343
                     /locus_tag="PK144_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67329.1"
                     /translation="LLMQHAVEDQALRARLLRAAQRYGSADPDLSRWRQALHQAVEAF
                     CEYPDHWDSDGNDDSEALCELADALTQRFDCGGEVPAALVEEAIDEFAACTEHAGEMA
                     GGELSDRIDALAELHMRIYRRTPPGPAALAAHLIELEVEDEHGIWRHTARHYAETLGA
                     TGLAEHRRLAAQALEDERAKPDTGNDGMYRVMRDILIDHAQLAGDLDTEIALLAERPD
                     GSTTWLYIAERLQAAGRSDEALAWAERGLVDDANHFFMDWRLRAFLIERYVERSRGAD
                     ALALAWSAFDRVPDLSGYQTLHGLAVRFGVLDDWRSRALARMGAMKPVWPGGDGATGE
                     GPEQTLRVEALCWEGEFDAAWAAAGAGRCSNSALEKLADASSASHPAEAAAVYQQLVS
                     HCVEQTHNFAYTEAIRRLHKLRPLLDAEAFAGYVAGLRTRYRLKRNFIRMLAEF"
     gene            complement(1427..2428)
                     /locus_tag="PK144_16275"
     CDS             complement(1427..2428)
                     /locus_tag="PK144_16275"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF67330.1"
                     /translation="MRYLPPAMAAARRRRALIAVLPPLALLALPLAAPLLAGHDGALP
                     WLLDLPVHWQWFGALWLALAGLFAALYADGRWALVLLGLVLPWLSAPPQLPARAETAA
                     RTLSIASANVEYLNHDATALRAWLAGVRPDLVVLLEVTPAWLASFGALDDWPYREIAA
                     SEGVHGIALLSRLPLGDVRVSRMAGSLASIDALVRLDDGRDIALAAVHLWPPMSAGLH
                     GARDRSLAHGLRALRAGGQPALLIGDLNATPWSPAFAPLQKHGWRRTTSLEPTWPDGP
                     LLALGLPIDHVLASPDWTRIEAAVGPAIGSDHRPVFVRLALSPLPGAAGASLAVPVR"
     gene            complement(2504..2677)
                     /locus_tag="PK144_16280"
     CDS             complement(2504..2677)
                     /locus_tag="PK144_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67331.1"
                     /translation="MSFAIYLIGYLIFLGGIAWALVTAGVPQLYVAIAIVILLGLGIF
                     TGAARTRGKDPSH"
     gene            complement(2828..3415)
                     /locus_tag="PK144_16285"
     CDS             complement(2828..3415)
                     /locus_tag="PK144_16285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF67332.1"
                     /translation="MSRVQKCLFWLLMAGLLAGFGWLLKPQIEAALYLSRLLAAEAPQ
                     HLPVPVAGIAPAQLRDSWGAPRSEGRRHQGIDIFAPRGRPVLSATEGIVLRVGENRLG
                     GLVVFVLGPGGQRHYYAHLDTQAPLEAGQRIQAGTPLGTVGTTGNARGTPPHLHYGIY
                     GAGGALNPYPLLRPPPAARSLPAATRPPLRPPTGS"
     gene            3687..>3980
                     /locus_tag="PK144_16290"
     CDS             3687..>3980
                     /locus_tag="PK144_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67333.1"
                     /translation="MRVMHVSMRAIAVGLARSPACHVFVRAPMPRKAASSAVMRTRVV
                     QAIATAPQAGARRRRPQTRRSPPGAVPVAPGGLRSAVLQAERILRSSSSTMVLR"
BASE COUNT          590 a         1374 c         1490 g          526 t
ORIGIN      
        1 agctgctgat gcagcacgcc gtcgaggacc aggccctgcg cgcgcgcctg ctgcgcgcag
       61 cgcagcgcta cggcagtgcc gaccccgatc tcagccgctg gcggcaggcg ctgcaccagg
      121 cggtcgaagc gttctgcgag taccccgatc actgggacag cgatggcaac gacgacagtg
      181 aagcgctctg cgaactggcc gatgcgctga cgcagcgttt cgactgcggt ggtgaggtcc
      241 cggcggcact ggttgaggag gcgatcgacg aattcgccgc ctgcaccgaa cacgccggcg
      301 agatggccgg tggcgagttg agcgaccgca tcgatgcgct ggccgaactg cacatgcgca
      361 tctaccggcg cacgccgccc gggcccgccg cgctggcggc gcatctgatc gaactcgaag
      421 tcgaggacga acacggcatc tggcggcaca cggcccgtca ctacgccgaa acgctcggtg
      481 ccaccgggct ggccgagcat cggcgccttg ccgcacaggc actggaggac gaacgggcga
      541 aaccggatac cggcaatgac ggcatgtatc gggtcatgcg cgacatcctg atcgaccacg
      601 cgcagctcgc cggcgatctc gataccgaaa tcgcgctgct cgccgagcgc ccggacggct
      661 cgacaacctg gctgtacatc gccgagcggc tgcaggcggc cgggcgcagt gacgaggcgc
      721 tggcctgggc cgagcgcggg ctcgtcgacg atgccaatca tttttttatg gactggcggc
      781 tgcgggcctt cctgatcgag cgctacgtcg agcgcagtcg cggcgccgat gcgctggcgc
      841 tggcctggtc ggcattcgat cgtgtgccgg atctgtcggg ctaccagacg ctgcacgggc
      901 tggccgtgcg cttcggtgtg ctcgatgact ggcgctcgcg ggcgctggcc cgcatggggg
      961 ccatgaagcc ggtatggccc gggggcgatg gtgccacggg cgagggaccg gaacagacgc
     1021 tgcgggtcga ggccctgtgc tgggaaggcg agttcgatgc cgcgtgggcg gccgccggtg
     1081 ccgggcgttg cagcaacagc gcgctcgaaa agctcgccga tgccagcagc gccagccatc
     1141 cggccgaggc ggcggccgta taccagcaac tggtcagcca ctgcgtcgaa cagacccaca
     1201 atttcgccta cacggaagcc atccggcggc tgcacaagct gcgcccgctg ctcgacgccg
     1261 aagcctttgc cggatacgtc gccggactgc gtacgcgtta ccgcctgaag cgcaatttca
     1321 tcaggatgct ggcggagttc tgataccggc cacacgcacc cctgtcccgc gagcggcgag
     1381 gggctgaaaa gacggcgtgt tcagccgtgt ccggcatgac cccgcttcag cgcaccggca
     1441 ccgccagact cgccccggcc gcgcccggca gcgggctgag ggcgaggcgc acgaataccg
     1501 ggcggtgatc ggagccgatc gccgggccga ccgcggcttc gatgcgcgtc cagtccggcg
     1561 aggccagcac gtggtcgatc ggcaggccga gggcgagcag cggcccgtcc ggccaggtcg
     1621 gttcgaggct ggtcgtgcgc cgccagccgt gtttctgcag cggcgcgaag gccggcgacc
     1681 acggcgtcgc gttcaggtcg ccgatcagca gcgccggctg tccgcccgct ctcagcgccc
     1741 gcaggccgtg cgcgagcgag cggtcgcgcg cgccgtgcag gccggccgac atcggcggcc
     1801 agagatgcac ggccgcgagc gcgatgtcgc ggccgtcatc gagccgcacc agcgcgtcga
     1861 tggacgcaag gctgcctgcc atgcgcgaca cgcgcacgtc cccgagcggc aggcgcgaca
     1921 gcagcgcgat gccgtgtacg ccctcgctcg cggcgatctc gcgatacggc cagtcatcga
     1981 gcgcgccgaa gctcgcgagc cacgccggcg tcacttcgag cagcacgacc agatcgggcc
     2041 gcacgccggc gagccaggcg cgcagcgccg tcgcatcgtg gttgaggtat tcgacgttgg
     2101 cgctggcgat gctcagcgtg cgcgccgcgg tctcggcgcg tgccggcagt tgcggcggcg
     2161 cgctcagcca cggcagcacc agcccgagca gcaccagcgc ccagcggcca tcggcgtaca
     2221 gcgctgcgaa caggccggcg agcgcgagcc acagcgcgcc gaaccactgc cagtgcaccg
     2281 gcagatcgag cagccacggc agcgcaccgt cgtggccggc gagcagtggc gcggcgagcg
     2341 gcagcgccag cagcgcaagc ggcggcagca cggcgatgag ggcccgccgc cggcgggcgg
     2401 cggccatggc ggggggcagg tagcgcatgc gcggggtttc cgttccgtgg gggcggcgca
     2461 tgcgccgccg gttcagcagc gggaaggaag gtttcgcccg gcctcagtgc gacggatcct
     2521 tgccgcgcgt gcgggccgcg ccggtgaaga tgccgaggcc gagcaggatg acgatggcga
     2581 tggccacgta caactgcggc acgccggccg tcaccagcgc ccaggcgatg ccgccgagaa
     2641 agatcagata gccgatgaga tagatcgcga aggacatggc gggaatcctc ggggggctgc
     2701 gccggcgatg ttgccggctt gccgcttcca gacctgcgcc ggcgcgtccg tgttcccgcg
     2761 gccgcaacgc ttgtcggtgc cgtgcaacag ccggccgtgc agccggcgtg tgccggtgtg
     2821 agcggtatca gctgccggtc ggcgggcgca gcggcggccg ggtcgcggcc ggcaggctgc
     2881 gtgcggccgg gggcgggcgc agcagcggat acggattcag cgcgccgccg gcgccgtaga
     2941 tgccgtagtg caggtgcggc ggcgtgccgc gggcgttgcc ggtggtgccg accgtgccaa
     3001 gcggcgtacc ggcctggatg cgctggccgg cttcgagcgg cgcctgcgtg tcgagatgcg
     3061 cgtagtaatg gcgctggccg ccggggccga gcacgaacac gacgaggccg ccgaggcggt
     3121 tctcgccgac gcgcagcacg atgccttcgg tggcgctgag caccggccgg ccgcgcggcg
     3181 cgaagatgtc gatgccctga tgccggcgcc cttcgctgcg cggcgcgccc cagctgtcgc
     3241 gcagttgcgc gggcgcgatg ccggcgaccg gcaccggcag gtgctgcggt gcctcggccg
     3301 ccagcagccg cgacagatac agcgccgcct cgatctgcgg cttcagcagc cagccgaaac
     3361 cggcgagcag gccggccatc agcagccaga acaggcattt ctgcacgcgg ctcatgcgca
     3421 ggttccgaag tcacggagag gccgtgcgcc ccgcaggcat cggacccgcg gcggcgggcg
     3481 gtgtttcccg gcagccgatg gtcgcccgcc gccgcggccc gctacgctgc gccggtggtg
     3541 gcgggcgagg gacgaacgat gcagcctcgc agggtgcgca tgaatgcgca cgcggtgcgg
     3601 cgtcaaagtc agacgtgaag cagcggggaa acgtcatcgc cggggtcgtc gggtttcctg
     3661 cgtcaaccca gcctacggtt gctgccatgc gcgtcatgca cgtttccatg cgcgccatcg
     3721 ctgtcggcct cgcccgcagc ccggcgtgcc acgttttcgt ccgtgctccg atgccccgca
     3781 aagcggcctc cagcgccgtg atgcgcacgc gggtggttca ggcaatcgcg actgcgccgc
     3841 aggcaggcgc gcgtcgtcgc cggccgcaga cacgccgaag cccgcctggc gcggttcccg
     3901 tggcgccggg cgggcttcgg tcagcggtgc ttcaggccga gcgcatcttg cgcagcagtt
     3961 cctcgacgat ggtgttgagg
//
LOCUS       DAOQIE010000338         3912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14206_length_3912_cov_9.396163, whole genome shotgun sequence.
ACCESSION   DAOQIE010000338 DAOQIE010000000
VERSION     DAOQIE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_3912_cov_9.396163"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1096)
                     /locus_tag="PK144_16295"
     CDS             complement(185..1096)
                     /locus_tag="PK144_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNF67334.1"
                     /translation="MTPFGIAGVQMHVTVGSNLEAMRHRIDLVMHLYPWVQMIVFSEL
                     CVHGPLLPHAQPLPGESEDELCTAARRHGIWLVNGSMFERRDGHIHNTTSVIDPQGTV
                     IGRYRKLYPFQPFEQGVTPGEAFLYWDVPGVGRFGVLNCYDIWFPENARTLAAHGVEV
                     ILHPVLTHTIDRDVDLCIARATAAMQQCYVFDINGLGAGGNGYSAVFDPHGRALYEGG
                     GSEQFIPVEIDLDQVRRSRQNGLRNLGQTLKSFRDRRVDYPIYDRSQADTAYLEALGK
                     LEKPAQGVPLTLGEPRDHGAPQPKVVF"
     gene            1338..2522
                     /locus_tag="PK144_16300"
     CDS             1338..2522
                     /locus_tag="PK144_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNF67335.1"
                     /translation="MANIHNDTFRWEDPLLFDAQLDHEERQIAAAAHDYCQGRLLPRI
                     REAHRHETFDRSIMTEMGELGLLGCTIEGYGCAGLSYVAYGLIAREIERVDSGYRSAA
                     SVQSSLVMHPIHAYGSEAQRRKYLPKLATGEWLGCFGLTEPDHGSDPGGMKTRARKID
                     GGWLLNGAKMWITNSPIADVFVVWAKDDSGKIRGFVLEKGMKGLSAPKIEGKFSLRAS
                     VTGEIVMSDVEVSDEHLLPGVQGLAGPFGCLNRARYGIAWGAIGAAEFCWHAARQYTL
                     DRKQFGRPLAATQLVQLKLADMQTEITLGLHAALRVGRLIDADQASPEMISLVKRNNC
                     GKALSIARTARDMHGGNGISDEYHVIRHVLNLEAVNTYEGTHDIHALILGRAQTGIQA
                     FC"
     gene            2781..>3912
                     /locus_tag="PK144_16305"
     CDS             2781..>3912
                     /locus_tag="PK144_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNF67336.1"
                     /translation="MSGPLAHLRVLDLSRVLAGPWAGQTLADLGADVIKIERPGCGDD
                     TRHWGPPYLKAADGTPTGEAAYFLSANRGKRSVTVDFTRPEGQAIVRALAAQSDVLLE
                     NFKVGGLAQYGLDADSLRALNPRLIYCSVTGFGQDGPYRDLPGYDFMIQGMGGLMSLT
                     GQPDGTPGGGPVKVGVALADVLTGLYATIGVLAALAHRERSGEGQHVDLALLDVQVAV
                     LANQALNYLTTGCAPQRLGNAHPNIVPYQTFAAADGHLILAVGNDAQFARCCAVLGCP
                     ELAADERFASNRARVAHRAELVPRLAACFATQPRDAWLAALANANVPCGPINTLDQVF
                     ADAQVQHRQMRVELPHAQAGTVALVGSPLKLSATPVEYHLPPP"
BASE COUNT          618 a         1357 c         1332 g          605 t
ORIGIN      
        1 cggaccttgc cccctctccc caacccctcc cccgcgaggg gggaggggct tgttcgtcaa
       61 tgccttgccg tcgtcctgat ccacccgcgc tgcccgggcg agtccgggag ccactgctgc
      121 aagctgtgca gatacgcaat gcccagaggg aaaaggactg caccgcgggg tcggcaatgt
      181 tctctcagaa caccaccttc ggctgcggcg cgccgtgatc gcgcggttcg ccgagcgtca
      241 ggggcacgcc ctgcgccggc ttttccagct tgccgagggc ttcgagatac gccgtgtcgg
      301 cctggctgcg atcgtagatc gggtaatcga cgcggcggtc gcggaagctc ttgagcgtct
      361 ggccgaggtt gcgcaggccg ttctggcggc tgcggcgcac ctggtcgaga tcaatctcca
      421 ccggaatgaa ctgttcgctg ccgccgcctt cgtacaaggc ccggccgtgc ggatcgaaca
      481 ccgccgaata accgttgccg ccggccccga gcccgttgat gtcgaacacg tagcactgct
      541 gcatcgccgc ggtcgcgcgc gcgatgcaga gatcgacatc gcggtcgatc gtgtgcgtca
      601 gcaccgggtg caggatcacc tcgacgccgt gcgcggccag cgtgcgggcg ttctccggga
      661 accagatgtc gtagcagttc agcacgccga agcgcccgac accgggcacg tcccagtaca
      721 ggaacgcctc gcccggcgtc acgccctgct cgaagggctg gaacggatag agcttgcggt
      781 agcggccgat caccgtgccc tgcgggtcga tgaccgaggt ggtgttgtgg atgtggccgt
      841 cgcgacgctc gaacatcgag ccgttgacca gccagatgcc gtggcgacgg gccgcggtac
      901 agagctcgtc ctcggactcg ccgggcagcg gctgggcgtg cggcagcagc ggaccgtgca
      961 cgcagagttc gctgaacacg atcatctgca cccacggata caggtgcatg acgaggtcga
     1021 tgcggtggcg catcgcttcg aggttgctgc cgacggtgac gtgcatctgc acgccggcaa
     1081 tgccgaaggg agtcatcggg gatcacctct gcgggacggg gaatcggacg aggtcttgcc
     1141 gccatgcgct acggaatatt gaacattccg gcgcacgcag ggatgctgtt ctgcaacact
     1201 tcggccgttt aatcaatgcc cgcgctgcat gacagcggca gagccgtgat cgtttgttga
     1261 atatttcaaa cggtgctagg ctcgttccgt ccccccgcct tcgcccgcgc gggcagcaga
     1321 cttccggaga cttgccgatg gccaacatcc acaacgacac cttccgctgg gaagacccgc
     1381 tgctgttcga cgctcagctc gatcacgagg aacgccagat cgccgctgcc gcgcacgact
     1441 actgccaggg ccggctgctg ccgcgcatcc gcgaggccca ccgccacgag accttcgacc
     1501 gttcgatcat gaccgagatg ggcgaactcg ggctgctcgg ctgcacgatc gaaggctacg
     1561 gctgcgcggg cttgtcctac gtcgcctacg gcctgatcgc ccgcgagatc gagcgcgtcg
     1621 actcgggcta ccgctcggcg gcgagcgtgc agtcctcgct ggtcatgcac ccgatccacg
     1681 cctacggcag cgaggcgcag cggcgcaagt acctgccgaa gctcgccacc ggcgaatggc
     1741 tcggctgctt cggcctcacc gaacccgacc acggctccga ccccggcggc atgaagacgc
     1801 gcgcccgcaa gatcgacggc ggctggctgc tgaacggcgc caagatgtgg atcaccaatt
     1861 cgccgatcgc cgacgtgttc gtggtctggg cgaaggacga cagcggcaag atccgcggct
     1921 tcgtgctcga aaaaggcatg aagggcctga gcgcgccgaa gatcgagggc aagttctcgc
     1981 tgcgcgcctc ggtgaccggc gagatcgtca tgagtgacgt cgaggtttcc gacgaacacc
     2041 tgctgcccgg cgtgcagggc ctggccgggc ctttcggctg cctgaaccgc gcgcgctacg
     2101 gcatcgcctg gggcgcgatc ggtgcggccg agttctgctg gcacgcggcg cgccagtaca
     2161 cgctcgatcg caagcagttc ggccggccgc tggcggccac gcagctcgtg cagctgaagc
     2221 tcgccgacat gcagaccgaa atcacgctcg gcctgcacgc ggcgctgcgc gtcggacggc
     2281 tgatcgatgc cgatcaggcc tcgccggaga tgatctcgct ggtcaagcgc aacaactgcg
     2341 gcaaggcgct ctccatcgcc cgtaccgcgc gcgacatgca cggcggcaac ggcatcagcg
     2401 acgagtacca cgtcatccgc cacgtgctga acctcgaagc cgtcaacacc tacgagggca
     2461 cgcacgacat ccacgcgctg atcctcggcc gggcgcagac cggcatccag gcgttctgct
     2521 gaggcgcgcg ctccgatcat gcgtgcggac ttcgtgagct tttctgcttc ccgtgtatcg
     2581 gtgacggcac cgacggctgt ggcggtctgc agtcctgttg ccggctggat gcttgccgac
     2641 aagctccgcg ccgtgacgag caagcccctc ccccctcgcg ggggaggggt tggggagagg
     2701 ggagaccgaa gcctcgcagg ctgtgtgatc tcccccggcc gcggcagggc tgccgcacca
     2761 cggatgtccc ggaggccggc atgagcggtc cgctggcgca cctgcgcgtg ctcgacctgt
     2821 cgcgcgtgct ggccggcccg tgggccgggc agacgctcgc cgatctcggc gccgacgtga
     2881 tcaagatcga gcgccccggc tgcggcgacg acacccgtca ctggggaccg ccgtacctga
     2941 aggccgccga tggcacgccg accggcgagg ccgcgtactt cctgtcggcc aaccgcggca
     3001 agcgttcggt gacggtcgat ttcacgcggc ccgagggaca ggcgatcgtg cgggcgctgg
     3061 cggcgcagtc cgacgtgctg ctcgaaaact tcaaggtcgg cggactggcg cagtacgggc
     3121 tggatgccga cagcctgcgg gcgctgaatc cgcggctcat ctactgctcg gtgaccggct
     3181 tcggtcagga cggcccgtac cgcgacctgc cgggctacga cttcatgatc cagggcatgg
     3241 gcgggctgat gagcctgacc ggccagccgg acggcacgcc gggcggcggt ccggtcaagg
     3301 tcggtgtcgc actcgccgac gtgctgaccg ggctgtacgc gaccatcggc gtgctcgcgg
     3361 cgctcgcgca ccgcgaacgc agcggcgagg gccagcacgt cgacctcgcg ctgctcgacg
     3421 tgcaggtcgc ggtgctcgcc aatcaggcgc tgaactacct gaccaccggc tgcgcgccgc
     3481 agcgcctcgg caacgcgcat ccgaacatcg tcccgtacca gaccttcgcg gcggccgacg
     3541 gtcacctgat cctcgccgtc ggcaacgatg cgcagttcgc gcgctgctgc gcggtgctcg
     3601 gctgcccgga actcgccgca gacgaacgct tcgccagcaa ccgcgcccgc gtcgcacacc
     3661 gcgccgagct ggtgccccgg ctcgcggcgt gcttcgcgac gcagccgcgc gatgcgtggc
     3721 tggcggcgct ggcgaacgcg aacgtgccct gcgggccgat caacacgctc gatcaggtgt
     3781 tcgccgatgc gcaggtgcag caccggcaga tgcgcgtcga gctgccgcat gcgcaggccg
     3841 gcacggtggc gctggtcggc agcccgctga agctctcggc cacaccggtc gagtaccacc
     3901 tgccgccgcc gc
//
LOCUS       DAOQIE010000339         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14260_length_3904_cov_7.110678, whole genome shotgun sequence.
ACCESSION   DAOQIE010000339 DAOQIE010000000
VERSION     DAOQIE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14260_length_3904_cov_7.110678"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="rlmJ"
                     /locus_tag="PK144_16310"
     CDS             complement(<1..746)
                     /gene="rlmJ"
                     /locus_tag="PK144_16310"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNF67337.1"
                     /translation="MLSYRHAFHAGNFADCLKHTLLLQLLDGLNRKDKPWLALDTHAG
                     AGLYELGAAQAQKTGEYRDGIARLWSRADLEPELAAYVAAVRSENPDGRLSRYPGSPA
                     LIRQRLRDRDRLIACELHPGDQPSLKALFAADRRIAVHKRDGYEAAGAFLPPPERRGL
                     LFVDPAYELAGEFERLLKLVGQVHRRWREGTVAIWYPILDRAPSERFCRALAASGVPA
                     LLRIELGLAPYDTPLGMKGSGVIVCNPPYQL"
     gene            complement(816..1910)
                     /gene="amrS"
                     /locus_tag="PK144_16315"
     CDS             complement(816..1910)
                     /gene="amrS"
                     /locus_tag="PK144_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNF67338.1"
                     /translation="MQRQLFTAPAPARYWHRLDDGRLQCDLCPRFCKLHDGSRGLCFV
                     RGRDGDALVLDTWGRSSGFCIDPIEKKPLNHWLPGTPVLSFGTAGCNLACKFCQNWDI
                     SKSRDTDRLAAQATPAMIAAAAADSGCRSVAYTYNDPVIFLEYAVEVAAACRARGIAS
                     VAVTAGYVCAGPRAEFFAAMDAANVDLKAFTEDFYHRVCGGHLAPVLETLVYLKRETR
                     VWFELTTLLIPGYNDGDDEIARLAEWVGRELGPEVPLHFTAFHPDWKLTDAPPTPPAT
                     LSRARRLAREAGLQYVYTGNVRDPEGASTWCPGCGAKLIGRDGRYRIDGWQLDAHGCC
                     RACGHALPGRFEAGPGDWGPQRLPLRLSAS"
     gene            2025..2849
                     /gene="amrB"
                     /locus_tag="PK144_16320"
     CDS             2025..2849
                     /gene="amrB"
                     /locus_tag="PK144_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNF67339.1"
                     /translation="MAISPARTLRPPAVAGLFYPEDPVELRASVTAALAGVATAAGAA
                     APKALIVPHAGYVYSGPIAASAYRRISRLHGRIERVVLLGPSHRIGFRGIALTSHAAY
                     VTPLGTVPIDREACASIAALPGVVELDAAHAQEHSLEVHLPFLQLTLGDFRLLPLVVG
                     DADAASVARVLERLWGGAETLVVVSTDLSHYHPYREAQQLDAATSRAIEQLRFESIGP
                     EQACGCRPLGGLLHLAREHGLTVSAIDLRNSGDTAGGRDRVVGYGAYVCHEHPREH"
     gene            2839..3438
                     /gene="amrA"
                     /locus_tag="PK144_16325"
     CDS             2839..3438
                     /gene="amrA"
                     /locus_tag="PK144_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNF67340.1"
                     /translation="MNIEALEHLSLEERAMLLRVARAAIDRGLASRQPPEVEVAAFPP
                     ALRELAATFVTLKLNGELRGCIGTLEARDPLILDVALHAYAAAFDDPRFLPVRRDEFE
                     RLDISVSVLTRPEPLKVSNQAELLEQLRPGRDGLILDYPGHRATFLPAVWKDLPDPLR
                     FVRNLKRKAGLTESFWSPQLRFLRYGSESFDEHDLAPAA"
     gene            3508..3583
                     /locus_tag="PK144_16330"
     tRNA            3508..3583
                     /locus_tag="PK144_16330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3541..3543,aa:Ala,seq:cgc)
     gene            3662..3737
                     /locus_tag="PK144_16335"
     tRNA            3662..3737
                     /locus_tag="PK144_16335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3695..3697,aa:Ala,seq:cgc)
BASE COUNT          583 a         1386 c         1347 g          588 t
ORIGIN      
        1 agctgatacg gcggattgca gacgatcacg ccgctgccct tcatgccgag cggcgtgtcg
       61 tagggcgcga gccccagttc gatgcgcagc agcgccggca cgccgctggc ggcgagggca
      121 cggcagaagc gttcggacgg cgctcggtcg aggatcggat accagatcgc caccgtcccc
      181 tcgcgccagc gccggtggac ctggccgacc agcttcagca gccgttcgaa ttcgccggcc
      241 agctcgtagg ccggatcgac gaacagcagc ccgcgccgct cgggcggcgg caggaaggca
      301 ccggcggcct cgtagccgtc gcgcttatgc accgcgatgc gccggtccgc ggcgaacagc
      361 gccttcagcg acggctgatc gcccggatgc agctcgcagg cgatcagccg gtcccggtcg
      421 cgcaggcgct ggcggatcag cgccggcgag ccggggtagc ggctgagccg gccgtcgggg
      481 ttttcgctgc gcacggcagc gacgtaggcg gcgagttccg gttcgagatc ggcgcgtgac
      541 cacagccggg cgatgccgtc gcggtattcg ccggtcttct gcgcctgcgc ggcacccagc
      601 tcgtagaggc cggcgccggc atgcgtgtcg agcgcgagcc agggcttgtc cttgcgattg
      661 aggccatcga gcagctgcag cagcagcgtg tgcttcaggc agtcggcgaa gttgccggcg
      721 tggaaggcgt ggcggtaact gagcatcggc gcgggctggc ggcggcaggc gggccggtca
      781 gcgtggacaa gcgcccggca gcacgcaagc cggactcacg atgccgacag ccgcagcggc
      841 aggcgctgcg gcccccagtc cccgggcccg gcctcgaagc gccccggcag tgcatgtccg
      901 caggcgcggc agcagccgtg cgcatcgagc tgccagccgt cgatccggta gcggccgtcg
      961 cgaccgatca gcttcgcgcc gcagcccggg caccaggtgc tggcaccttc ggggtcgcgg
     1021 acgttgccgg tatagacgta ctggagcccg gcctcgcggg cgagacggcg tgcgcggctc
     1081 agcgtcgccg gcggcgtcgg cggggcgtcg gtgagcttcc agtccgggtg aaaggccgtg
     1141 aagtgcagcg gcacttcggg tccgagttcg cggcccaccc actcggcgag gcgcgcgatc
     1201 tcgtcatcgc cgtcgttgta gccggggatc agcagcgtcg tcagctcgaa ccagacccgg
     1261 gtctcgcgct tcaggtacac gagcgtctcc agcaccggcg ccagatgtcc gccgcagacg
     1321 cggtggtaga aatcctcggt gaaggccttc agatcgacgt tggccgcgtc catcgccgcg
     1381 aagaactcgg cgcgcggccc ggcgcagacg tagccggccg tcaccgcgac gctggcgatg
     1441 ccgcgcgcgc ggcaggcggc ggcgacctcg accgcgtatt cgagaaagat caccgggtcg
     1501 ttgtacgtgt acgcaacgct gcgacagccg ctgtccgcgg ccgccgcggc gatcatcgcc
     1561 ggcgtcgcct gcgcggcgag ccggtcggtg tcgcgcgact tcgagatgtc ccagttctgg
     1621 cagaacttgc atgccagatt gcagccggca gtgccgaagg acagcaccgg cgtgcccggc
     1681 agccagtggt tcagcggctt cttctcgatc ggatcgatgc agaagcccga cgagcgcccc
     1741 caggtgtcga gcaccagcgc atcgccgtca cgcccgcgca cgaagcagag gccgcgactg
     1801 ccgtcgtgca gcttgcagaa gcgcggacac aggtcgcact gcaggcggcc atcgtcgagc
     1861 cggtgccagt aacgggccgg tgcgggggct gtgaacagtt gtcgctgcat ggcaacaccc
     1921 tctgtacctg cgactcatat cagggaaata ttagaattcg ttcagaattc gacggagtgt
     1981 cgatccagtc cgagtggagg ccgcagtccg ctgcgacaaa gacgatggcg atcagccctg
     2041 cccgcaccct gcgcccgccg gccgttgccg gcctgttcta cccggaagat ccggtggaac
     2101 tgcgggccag cgtcacggcg gccctcgccg gagtcgccac cgccgccggt gccgccgctc
     2161 cgaaggcgct gatcgtgccg cacgccggct acgtctattc gggaccgatc gccgccagcg
     2221 cctatcgccg gatcagccgg ctgcacggac gcatcgaacg cgtcgtgctg ctcggcccga
     2281 gtcatcgcat cggctttcgc ggcatcgcgc tgacgagtca cgcggcttat gtgacaccgc
     2341 tcggcacggt tccgatcgac cgcgaagcct gcgcgtcgat cgcggccctg cccggcgtcg
     2401 tcgaactcga cgccgcccat gcgcaggagc acagcctgga ggtccacctg cccttcctgc
     2461 agctgacgct cggcgacttc cggctgctgc cgctggtcgt cggcgatgcc gatgccgcca
     2521 gcgtcgcccg cgtgctggaa cgcctctggg gcggcgcgga aaccctggtc gtggtgagca
     2581 cggatctcag ccactaccac ccttacaggg aagcgcagca actcgatgcc gccacgtcgc
     2641 gcgccatcga acagctgcgg ttcgagtcga tcggccccga gcaggcctgc ggctgccgtc
     2701 ccctgggcgg tctgctgcac ctcgcccgcg agcacggtct gacggtgtct gccatcgatc
     2761 tgcgcaattc cggcgatacc gccggcggac gcgaccgggt cgtcggatac ggtgcttacg
     2821 tctgccatga acacccccgt gaacattgaa gccctcgaac acttgagcct cgaagagcgc
     2881 gcgatgctgc tgcgtgtggc gcgggccgcg atcgatcgcg gcctggcctc gcgccagccg
     2941 cccgaggtcg aggtggccgc cttcccgccg gcgctgcgcg agctcgctgc gaccttcgtg
     3001 acgctgaagc tcaacggcga gctgcgcggc tgcatcggca cgctcgaagc ccgcgatccg
     3061 ctgatcctcg atgtcgcgct gcacgcctac gccgccgcct tcgacgatcc gcgcttcctg
     3121 ccggtgcgcc gcgacgagtt cgagcggctc gacatctcgg tgtcggtgct gacgcgcccg
     3181 gagccgctga aggtcagcaa ccaggccgaa ctgctcgaac aactgcgccc gggccgcgac
     3241 ggcctgatcc tcgactaccc cggccaccgc gcgaccttcc tgccggcggt ctggaaggac
     3301 ctgcccgacc cgctgcgctt cgtgcgcaac ctcaagcgca aggccggcct gaccgaaagc
     3361 ttctggtcgc cgcagctgcg cttcctgcgc tacggcagcg aatccttcga cgagcacgac
     3421 ctcgcacccg cggcctgaat cccggccgca gggctggcga gccggggggg cgatgtgtat
     3481 gatgcgcgcc tcacccgcaa gggtaacggg gcggtagctc agctgggaga gcgtcgcgtt
     3541 cgcaatgcga aggtcgaggg ttcgatcccc ttccgctcca ccaacaccca cccgccggct
     3601 tccagagccg gcgggttttt tcaccccggc acccacaccg ggcagacgat caacaggttt
     3661 cggggcggta gctcagctgg gagagcgtcg cgttcgcaat gcgaaggtcg agggttcgat
     3721 ccccttccgc tccaccaatc attcaacgcc cgaccctcac cggtcgggcg ttttgttttg
     3781 gcggccgcgg ccccgctctg tgccagctcc ttgaatccgg cgatgacgct cgcccgcgac
     3841 ggcggtgcag ccgtgtgcat gccgtgcgga cccgcagcgg tcgcactgag cctcgacgct
     3901 gacg
//
LOCUS       DAOQIE010000340         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14346_length_3887_cov_5.022182, whole genome shotgun sequence.
ACCESSION   DAOQIE010000340 DAOQIE010000000
VERSION     DAOQIE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14346_length_3887_cov_5.022182"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1434)
                     /gene="pyk"
                     /locus_tag="PK144_16340"
     CDS             complement(13..1434)
                     /gene="pyk"
                     /locus_tag="PK144_16340"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNF67341.1"
                     /translation="MRRICNARIVATLGPSSSTPEVIRALAEAGADVFRFNFSHGSHE
                     DHRARMQVVRDIEKAVGRPIGVLLDLQGPKLRVGTFAQGPVTLVKGRNFRLDLDPAPG
                     DATRAPLPHPEIFAALKPGADLLIDDGRVRLHVERCGADFAETTVVAGGMVSNRKGVN
                     VPSVVLPISPLTAKDRADLECGLELGVDWVALSFVQRPEDMDELRALVGNRAGILAKL
                     EKPSAIEQLTAIVERSDAVMVARGDLGVELPPERVPGIQKRIIHAARSAGRPVIVATH
                     MLDSMVHAPVPTRAEASDVANAIFDGADAVMLSAESASGDYPVEAVRMMDRIIHEVER
                     DPAWRRLMDANQAVPEATTADAVCAALAQVTQVLGIAATVTYTSSGYTALRAARERPE
                     APILGLTPNLATARRLTLVWGVTPVHGEDIHDVAEMVEHAKRAAVAHGYAGPGDRLAI
                     VAGMPFAQPGTTNLLHLAQIGEG"
     gene            1720..2181
                     /locus_tag="PK144_16345"
     CDS             1720..2181
                     /locus_tag="PK144_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNF67342.1"
                     /translation="MTAAASEKLYLDDLVPGARYVSAGHALDAAQIKTYAGDFDPQYF
                     HLDEAAAQGSLFGGLAASGWHTAAITMRLLVTSGLPLAGGLIGAGVELSWPQPTRPDD
                     VLHVESEILSVTPSRSRPDRGMVAVRSETKNQRGEVRQVMVAKMVVPRRIV"
     assembly_gap    2350..2449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2636..3013)
                     /locus_tag="PK144_16350"
     CDS             complement(2636..3013)
                     /locus_tag="PK144_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNF67343.1"
                     /translation="MKVLLDTHIVLWSLAVPERLSTAARNTIESAEEIYVSSVSIWEI
                     SIKSGLGKLDVDVDELLGWLVSLKIVSLPVTWSHARALRELPLHHRDPFDRMLIAQAV
                     TEPLHLLTADVQLKPYSSLVLCQ"
     gene            complement(3010..3255)
                     /locus_tag="PK144_16355"
     CDS             complement(3010..3255)
                     /locus_tag="PK144_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNF67344.1"
                     /translation="MKTVNIHEAKTHLSKLLDQVAAGEEITIARAGKPIARLAPLDLP
                     RKPFAFGAMRGRIRIADDFDAPLPDDLLSMFESGEPS"
     gene            complement(3329..>3887)
                     /locus_tag="PK144_16360"
     CDS             complement(3329..>3887)
                     /locus_tag="PK144_16360"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF67345.1"
                     /translation="AELLRRRPDVLRAEAELAAASARIGVAEAELYPKLTLSGTLGIA
                     ATQVSNLGPSGAQTWSFGPALSLPIFEGGRLRSVVAAREAALDGARARYEQTVLGAVG
                     EVETAAATLTETRARRAALVRSLAALQRALGHADDRYRAGVSSFLDVLDAQRALFVTE
                     IEVARADRDLAAAQVRLHKALGQGW"
BASE COUNT          650 a         1289 c         1280 g          568 t
ORIGIN      
        1 ggcagggacg gctcagccct cgccgatctg cgccagatgc agcaggttgg tcgtgcccgg
       61 ctgcgcgaac ggcatgccgg cgacgatggc gaggcgatcg ccggggccgg cgtagccgtg
      121 ggcgaccgcg gcgcgcttgg cgtgctcgac catctcggcg acatcgtgga tgtcctcgcc
      181 gtgcaccggt gtcacgcccc agaccagcgt cagccggcgc gcggtcgcga ggttcggcgt
      241 caggccgagg atcggggctt cggggcgctc gcgcgccgcg cgcagcgccg tgtagccgct
      301 gctggtgtag gtgacggtcg cggcgatgcc gagcacctgc gtgacctgcg ccagcgccgc
      361 acagacggca tcggcggtgg tcgcctccgg caccgcctgg ttggcatcca tcagccgccg
      421 ccaggccggg tcgcgttcga cctcgtggat gatgcgatcc atcatccgca ccgcctcgac
      481 cgggtagtcg cccgaggccg actcggccga cagcatcacc gcatcggcgc cgtcgaagat
      541 ggcgttggcg acatcggaag cctcggcgcg ggtcggcacc ggtgcatgca ccatcgaatc
      601 gagcatgtgc gtggcgacga tcaccggtcg cccggcgctg cgcgcggcgt ggatgatgcg
      661 cttctggatg cccggcacgc gctcgggcgg taactccacg ccgagatcgc cgcgcgcgac
      721 catcaccgcg tcggagcgct cgacgatggc ggtgagctgc tcgatcgccg acggcttttc
      781 gagcttggcg aggatgccgg cgcgattccc gaccagcgcg cgcagctcgt ccatgtcctc
      841 gggccgctgc acgaaggaca gcgccaccca gtcgacgccg agttccagcc cgcattcgag
      901 atcggcgcga tccttcgccg tcagcggcga gatcggcagc accaccgagg gcacgttgac
      961 gcccttgcgg ttcgagacca tgccgccggc aacgacggtg gtctcggcga aatcggcgcc
     1021 gcagcgctcg acgtgcaggc gcacgcggcc gtcgtcgatc agcaggtcgg ccccgggctt
     1081 cagtgccgcg aagatctccg gatgcggcag cggcgcgcgc gtggcatcgc cgggcgccgg
     1141 atcgagatcg agccggaaat tccggccctt cacgagcgtc accggcccct gtgcgaacgt
     1201 gccgacacgc agcttcggcc cctgcaggtc gagcagcacg ccgatcggcc ggccgacggc
     1261 cttctcgatg tcgcgcacga cctgcatgcg cgcccgatgg tcctcgtggc tgccgtggct
     1321 gaagttgaag cggaacacgt cggcaccggc ctcggcgagg gcacggatca cctcgggcgt
     1381 ggaactcgaa ggtccgagcg tggcgacgat gcgggcgttg cagatgcggc gcatggcagg
     1441 tactccgggg cggacggcag gcgaaacacc ggcagccggg cttcggttcc tcggcagcgg
     1501 cagcgcgagg cgccgctgcg tcggcggtcc ggacggaccg cgcccgggaa tgtagccgtc
     1561 cgctccccgc aaggcttgct gcagccgcag cagccgcagc cgtcacccgg gccgcggacc
     1621 cggcgcgccg cgcctgcatc gcagccatgc cggccttgcc gggcgcgggt ccgtcgacca
     1681 aacctgccgc gaatcgccac ccgcctgcag gaacccgcca tgaccgctgc cgcctccgaa
     1741 aagctctatc tcgatgatct cgtgcccggt gcgcgctacg tcagcgccgg acacgcgctc
     1801 gatgccgcgc agatcaagac ctacgccgga gacttcgatc cgcagtactt ccacctcgac
     1861 gaggccgccg cgcagggctc gctgttcggc ggcctcgccg cgagcggctg gcacacggcc
     1921 gcgatcacga tgcgcctgct cgtgaccagc ggcctgccgc tcgccggcgg cctgatcggc
     1981 gccggcgtcg aactgagctg gccgcagccg acccggccgg acgacgtgct gcacgtcgaa
     2041 agcgaaatcc tctcggtgac cccgtcgcgc tcccggccgg atcgcggcat ggtcgcggtc
     2101 cggagcgaga cgaagaatca gcggggggag gtaaggcagg tgatggtggc gaagatggtg
     2161 gtgccgcggc ggatcgtctg aaaccggcaa agctgaaacc gacagaatga agcccgtcca
     2221 ctcgcgtgca attctcgaaa gaacgggctc cactgaattt gtcaaatgaa tttgtcaaac
     2281 ttgcgaacag aaacgctgtt cagcttgccg gtcgcaaggc aggcagctca cggctctgga
     2341 atccagaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctgttcagct
     2461 tgccggtcgc aaggcaggca gctcacggct ctggaatcca gaaacctgaa cgcccggcac
     2521 aacccgtagg gtggtcctgg aaatcaggaa accagacgtg aaccgaaatt ctcgtgagag
     2581 cctgagcatt gcccatgcac gccaagctga acgcatgaat ccatgaatgc atgcatcact
     2641 gacaaagcac caaagatgaa taaggcttca gctgaacatc agccgtcagc aaatgcaggg
     2701 gctcggtgac cgcctgagcg atcagcatgc ggtcgaaagg atcgcgatga tgcagaggca
     2761 actcgcgcaa ggctctggcg tgagaccatg tgaccggcaa cgacacgatc ttgagactga
     2821 cgagccagcc gagcagctca tcaacatcga catccagctt gcccagccct gacttgatac
     2881 tgatctccca gatggatacc gaactcacgt aaatctcttc ggcactttcg atggtatttc
     2941 gcgccgctgt ggaaagtcgc tccggcactg ccaggctcca gagcacgata tgcgtgtcga
     3001 gcagcacctt cacgaaggtt cgccgctttc aaacatcgac agcaagtcat ccggcagcgg
     3061 cgcatcgaaa tcatccgcga tcctgatccg gccgcgcatg gcaccgaagg caaacggttt
     3121 cctgggtaaa tccagtggcg caagccgggc gatcggtttg ccggcacgcg caatcgtgat
     3181 ttcttcgcct gcggcaacct gatccagcag ttttgacaag tgcgtcttgg cttcgtggat
     3241 attgacggtt ttcatgttgc cccctgtacg cgagaactag tccagcttag tccagtacag
     3301 tcgcatccgg caggacggca aaccagactc accacccctg ccccagcgcc ttgtgcagcc
     3361 gcacctgggc cgccgccaga tcgcggtcgg cgcgggcaac ctcgatttcg gtgacgaaca
     3421 gcgcgcgctg cgcgtcgagc acgtcgagga agctcgacac gccggcacgg tagcggtcgt
     3481 cggcgtggcc gagggcgcgc tgcagcgcgg cgagcgagcg gaccagcgcg gcgcggcggg
     3541 cgcgggtttc ggtcagggtg gcggcagcgg tttcgacctc gccgacggcg ccgagcacgg
     3601 tctgttcgta gcgggcgcgg gcgccgtcga gcgcggcttc gcgggcggct acgacactgc
     3661 gcagccgtcc gccctcgaag atcggcagcg acagcgccgg gccaaagctc caagtctgcg
     3721 cgcccgacgg gccgaggttc gagacctgcg tcgccgcgat gccgagcgtg ccgctcagcg
     3781 tcagcttcgg atacagctcg gcctcggcca ctccgatgcg cgcactcgcg gccgccagtt
     3841 cggcctcggc gcgcagcacg tcgggccggc ggcgcagcag ctcggcc
//
LOCUS       DAOQIE010000341         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14426_length_3873_cov_7.598481, whole genome shotgun sequence.
ACCESSION   DAOQIE010000341 DAOQIE010000000
VERSION     DAOQIE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14426_length_3873_cov_7.598481"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            685..1182
                     /locus_tag="PK144_16365"
     CDS             685..1182
                     /locus_tag="PK144_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67346.1"
                     /translation="MSESSSEPATPLSGTEHGFLDDLCSAIPAKTDYAILGFDVHLHC
                     CYASGPGLGALGIENPPRGTGLQTLAPALRTVVSGLCRCVFAGALTETEITHGPRRLR
                     LNGLPLLDSNGAISMALIRILPAEPASGARRDTATGFRQRGCDEATDWHGPAGALRQD
                     AAHRR"
     gene            1329..2423
                     /locus_tag="PK144_16370"
     CDS             1329..2423
                     /locus_tag="PK144_16370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNF67347.1"
                     /translation="MEARYGFEAAERLARLHAAGLALERLTLALGNAETAAPAAREAA
                     LAASHTAWQQFSLAFSALGAGLAADSTLHHDYAAFERQVASRIAVLAPPDPGPAPGRP
                     PEGRPGEPAEPYRSASARLHAHTAAEAQQLTDAADIHRLRLEHYLQWLGGLLSGSALL
                     ALHLVRRRSADWQQRLRHLEYEATHDPLTQLPNRRLFTDCAVWGLAHALRCQRPACVL
                     LLDLDGFKAVNDRYGHAAGDRLLIDIAARLQQCLRESDLLARLGGDEFAILIPSSCSI
                     GDPDRLAVRLLQSIRTPQPDAAGRVPISASIGIACLPGHGEDIHTLLQAADTAMYAAK
                     RAGGNAFRHAATPAESPPCERAADADSRTA"
     gene            2948..3151
                     /locus_tag="PK144_16375"
     CDS             2948..3151
                     /locus_tag="PK144_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67348.1"
                     /translation="MSKRLHTALRGIGSVLAIAPGPGVAGFRERVRRVRLYRDDREAL
                     RSDFERVAADLRKVLDRIGNADH"
     gene            3138..3623
                     /locus_tag="PK144_16380"
     CDS             3138..3623
                     /locus_tag="PK144_16380"
                     /inference="COORDINATES: protein motif:HMM:NF021602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HNF67349.1"
                     /translation="MPTTEETGAGHHDGVRPVTPAVPPARTPDLPAAVADDLSTINSA
                     AAHMVQVAAQAFSGPLPPPDLLEHYQRIQPDLVERLMRLTESEAAHRHALETQALAAS
                     ERIEARGQRYGLLIGLAALAVAGMALFLGHEKAAMTIGGTTVVGLATVFVVGRWKKPS
                     E"
BASE COUNT          599 a         1495 c         1258 g          521 t
ORIGIN      
        1 cgcaagctcg gggccggcac gctgaccgag ctcgtgcagc tgcacgtggc cgcgagcacg
       61 aacggcaccg acccgcgcac gccgtatcac gccctcgacc tctggatgcc gccgcgacga
      121 cctgcggacg acggctgaat ggccgggagc aagcggcagc ctgtcagcac gacccgttgc
      181 ggcgcggcgt ccggatacca ccgttcacgc agcgcacgcg ccacggcgca gccgttcatc
      241 cccggcatga cgggattgcc agcacgggat cgtcgagacc ggcgtcgatc gttccctcct
      301 ccgcgccgct gatccgccgg ccgcttcaag tttcccccga aaaagtaggt acaaaccggt
      361 agccgccggg gaaagcgcca atggtgcttt gcccttcctg ccagcacagt gacgccagcc
      421 ccgcagtccg ggccacgaca gggagggtgg caggcagcga gacggggcca agagaagtga
      481 cgatacgtcc ccggaggagc gggacacgcg ccagcgtgtt gcgccagaag tagcgcggcg
      541 gttcgggacg agcctccgcg ccgcttttgc taggcatttc ccgcacgtca ccgtcgcttc
      601 cagcgccatt gcccgggccg gagcctgtcc ggcgacggcg gtggcccgcc ggatcgcatc
      661 acctgggaaa aggggaagcc tgtcatgtcc gaatcttcgt ccgaacctgc cacaccgctg
      721 tccggaacag agcacggttt tctcgacgac ctctgctccg cgatccccgc aaagaccgac
      781 tacgccatcc tcggcttcga cgttcacctg cactgctgct acgccagcgg cccgggactc
      841 ggcgcgctcg gcatcgaaaa tccgccccgc ggcaccggcc tgcagacgct cgcaccggcg
      901 ctgcgcacgg tcgtgagcgg gctgtgccgc tgcgtcttcg ccggtgcgct gaccgaaacc
      961 gagatcacgc acggcccgcg gcggctgcgc ctgaacggcc tgccgctgct cgacagcaat
     1021 ggtgcgatca gcatggccct gatccgcatc ctgcccgccg agccggcctc cggcgcccgg
     1081 agggacacgg ccaccggctt ccgccagcgc ggctgcgacg aggccaccga ctggcacggc
     1141 cctgccggcg cgctccggca ggatgccgcc cacagacgct gagtgccggc ggtgtaccga
     1201 tgccggagcc ggcagcgctt ccgtctctgc gcacgtccgt gccgatccgg cggtccccgc
     1261 gccgccgctt cctcgcgctc gtcacgggca tcgcccttgt gctcaccgct ctgggcggac
     1321 tcgtggtcgt ggaggcccga tacggtttcg aggccgccga gcgcctcgcc cggctgcacg
     1381 cggccgggct cgccctcgaa cgcctgacgc tcgccctcgg caacgccgag accgccgcgc
     1441 cggccgcgcg cgaagcggcc ctcgccgcca gccacaccgc ctggcagcag ttcagcctcg
     1501 cgttctccgc gctcggcgcc ggccttgccg ccgacagcac gctgcaccac gactacgccg
     1561 ccttcgaacg gcaggtcgcg agccgcatcg ccgtcctcgc gccgcccgac cccggaccgg
     1621 ctcccgggcg ccctcccgag ggccggcccg gcgaacctgc agagccatac cgcagcgcca
     1681 gcgcccggct gcacgcccat accgcggccg aagcgcagca gctcaccgat gccgccgata
     1741 tccatcgcct gcgcctggag cactacctgc agtggctcgg cggcctgctg tccggcagcg
     1801 ccctgctcgc cctgcatctg gtccgtcgcc ggtcggcgga ctggcagcaa cgcctgcggc
     1861 atctggaata cgaggcgacc cacgatccgc tgacgcagct gccgaaccgg cgcctgttca
     1921 ccgactgcgc cgtctgggga ctcgcgcacg cgctgcgctg ccagcggccg gcctgcgtgc
     1981 tgctgctcga tctggacggc ttcaaggccg tcaacgaccg ctacggccac gccgccggcg
     2041 accgcctgct catcgacatc gccgcgcgcc tgcagcagtg cctgcgcgaa tccgacctgc
     2101 tcgcccggct cggcggcgac gaattcgcca tcctcatccc gagcagctgc agcatcgggg
     2161 atccggaccg gctcgccgtg cgcctgctgc agagcatccg cacgccgcag cccgacgccg
     2221 ccggccgggt gccgatcagc gccagcatcg gcatcgcctg cctgcccgga cacggcgagg
     2281 acatccacac cctgctgcag gccgccgaca cggcgatgta cgccgccaag cgcgcgggcg
     2341 gcaacgcctt ccgccacgct gcgacgccgg cggaatcccc gccctgcgaa cgggccgccg
     2401 acgccgactc ccggacggcc tgaagcgcag cggatgcccg ccgcggcgcc cctgcaaaaa
     2461 cgcccggccc cagaaggcgg attccgtgcc cggcagggcg cgacggcgac gcgacggcca
     2521 tcacctgcca ttcccgctcg aacgcgcacg gcggaggctg gccgattggc catgatttgc
     2581 cacgtcgctg ccaattggac agcctcgtgc aaattgcagc caatgacctt gcgccctgcc
     2641 gcccctgatc cgtggccctg cctgcgcacc ggaccggcgg atgccatgcc cggtgtcgga
     2701 tcactcctga tcctgcaggc gcccgccggg cgcgagcggc acatgcagcg ggaacgccgg
     2761 ctgcggccgg gccgcccaca ttccgggcac ccttcgaccg gatcgccatg gcatcggccc
     2821 tgaatacacc ctgcgcgcgg gtggcgaggc tggaagctgc ctgcgatggc acgtccgcca
     2881 ccagccgtca ccgtatactg tccatgaatc gcccatgccc cgaaggccac ccagggaatc
     2941 gcagtccatg agcaaacgcc tacataccgc gctgcgcggc atcggcagcg tgctcgccat
     3001 cgcgcccggt ccgggcgtgg ccggcttccg cgagcgcgtg cgccgcgtgc gcctgtaccg
     3061 tgatgaccgc gaggcgctgc gcagtgattt cgagcgcgtc gcggccgacc tgcgcaaagt
     3121 gctggaccgg attggcaatg ccgaccactg aggaaaccgg cgccgggcat cacgacggcg
     3181 tccggcctgt aacgcccgcc gtaccgcctg cccgcactcc tgacctgccc gccgcggtcg
     3241 ctgacgacct gagcacgatc aacagcgctg ccgcacacat ggtgcaggtg gcagcacagg
     3301 ctttcagcgg cccgctaccg ccgcccgacc tgctcgaaca ctaccagcgc atacagcccg
     3361 atctggtcga gcggctgatg cgcctgaccg aatccgaagc cgcacaccgc cacgccctcg
     3421 aaacgcaggc gctcgcagcg tccgagcgta tcgaggcacg cggacaacgg tacggcttgc
     3481 tgatcggcct ggcagcgctc gccgtggcgg gcatggcgct gtttctcggt cacgagaaag
     3541 ccgcgatgac gataggcggc acaaccgttg tcgggctggc gacggtgttc gtcgtcggac
     3601 gctggaagaa gccgtccgag tgatacccat cccatagctg atcggcatga cgagaccggc
     3661 cgggcagccg gacggaaggc agcgttcgcg ccggtggcga gcacggcatc gaaccagcac
     3721 cggccacccg catgccggcg ccgtccagaa tgacgcgcca gccgcatgac cgcgggtccc
     3781 ggggtcacag tcacggcttg agggtgagcc ctgcgtgacc ccgaaaagga caagggccta
     3841 ggttttcacc taagcccttg atttttggtg gct
//
LOCUS       DAOQIE010000342         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14428_length_3873_cov_6.254322, whole genome shotgun sequence.
ACCESSION   DAOQIE010000342 DAOQIE010000000
VERSION     DAOQIE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14428_length_3873_cov_6.254322"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PK144_16385"
     CDS             <1..1348
                     /locus_tag="PK144_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF67350.1"
                     /translation="LRRELAPADAWTGAAYVSATLAAAALVLWQAGEPRLAAWVLAGT
                     AATAVLLALGAWGLTRLLGRLRGRVGVAWRFGLANVSRRGGASIVQVVAIGLGLMVLL
                     VLTLVRTDLLAGWRASLPPEAPNRFLINVQPGEVEALQAELHAAGLGAATLHPMVRGR
                     LTQIGERPVSAADYPEPRAQRLVEREFNLSWAEAPQPDNRIVAGRWFGPGARAEVSVE
                     QGLAETLGIRLGDRLRFAVAGQSVEGTVTSLRSVEWDSFHVNFFVVFPPGALDGLPAT
                     WITSFHLPAGRQALIAELVRAHPSVTVLDIDALLSKVREIIERVVLGVEYVFLFTLAA
                     GLAVLYAAIQATRDERRYEAALARTLGASRRVVLQGLIAEFAVLGLLAGVLAAAGASA
                     LGWGLAQQVFDFPYRPDPLLWLAGAAAGVFGVGLAGLAGTRAVLDQPPLATLRAGV"
     gene            1439..1789
                     /locus_tag="PK144_16390"
     CDS             1439..1789
                     /locus_tag="PK144_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNF67351.1"
                     /translation="MDTPSEDPRARLERLASELDQRLARIGARERHEDGPPSQDFAEQ
                     ATERENDDVIGALALDTRLQLGLVRKALRRLDAGDYGVCVECGTPIPSARLAALPWAE
                     HCVACAERLEGRHA"
     assembly_gap    1791..1890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1891..2573)
                     /locus_tag="PK144_16395"
     CDS             complement(<1891..2573)
                     /locus_tag="PK144_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67352.1"
                     /translation="MPGTTPLLPVDRWLSPAGGLVYHLRALRHRPLWRAYSDRLAGWL
                     QAWNPPCRELVLIGPSAGWTLPAAFLAGFGQVHVLEPDPLARRLLGRRFADTKLVFDR
                     LDCLCDRDGPARLARAYPDAALLFANVLGQQVAGPVIADPGRLRRALAGQHWASCHDV
                     LSAARAPHGALPDTAAGLDAAALAEHLWGGSGLEVCDHDTLGLGGAPGPLALWPLTPR
                     AWHVIEWIV"
     gene            complement(2724..3656)
                     /gene="cysK"
                     /locus_tag="PK144_16400"
     CDS             complement(2724..3656)
                     /gene="cysK"
                     /locus_tag="PK144_16400"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNF67353.1"
                     /translation="MRIARNVTELIGNTPLVRLNRLTAGLGAEVVCKLEFQNPAHSVK
                     DRIGAAMIDAAEAAGKIGPDTVILEPTSGNTGIALAMVCAARGYKCCLVMPDTMSRER
                     RMLLRGYGADLVLTPGAEGMAGAIRKAEELAAGNPNYYIPQQFQNPANPEVHRRTTAE
                     EIWRDTDGEIDIFVAGIGTGGTITGVGEVLKPRRPGLKIVAVEPDASPVLSGGQKGPH
                     IIQGIGAGFVPEVLNTGIYDEVTRVTNDDALVTARRAAREEGLLVGISSGAALWAALQ
                     QAAKPENAGKRIVVIIPSFGERYLSTKLFEGLGD"
BASE COUNT          463 a         1347 c         1418 g          545 t
ORIGIN      
        1 gctgcgccgc gagctggccc cggccgacgc ctggaccggc gccgcctacg tgtcggcgac
       61 gctcgccgcc gcggcgctgg tgctgtggca ggccggcgag ccgcggctcg ccgcctgggt
      121 actggccggc accgccgcga ccgccgtgct gctcgcgctc ggggcctggg ggctgacccg
      181 gctgctcggc cggctgcgcg gccgggtcgg ggtggcctgg cgcttcgggc tcgcgaatgt
      241 ctcgcgtcgc ggcggcgcca gcatcgtgca ggtggtcgcg atcgggctcg ggctgatggt
      301 gctgctggtg ctgacgctgg tgcgcaccga cctgctcgcc ggctggcgcg caagcctgcc
      361 gcccgaggcg ccgaaccgct tcctgatcaa cgtccagccc ggcgaggtcg aggcgctgca
      421 ggccgaactg catgccgccg ggctcggggc cgcgacgctg catccgatgg tgcgcggccg
      481 gctgacgcag atcggcgaac ggccggtgtc ggccgccgac tacccggagc cgcgcgcgca
      541 gcgcctggtc gagcgcgagt tcaacctgtc ctgggccgaa gcgccgcagc cggacaaccg
      601 catcgtcgcc ggccggtggt tcggtcccgg ggcgcgcgcc gaggtctcgg tcgagcaggg
      661 actggccgag acgctcggca tccggctcgg cgaccggctg cgcttcgcgg tcgccggcca
      721 gagcgtcgag ggcacggtca cgagcctgcg cagcgtcgag tgggattcct tccatgtgaa
      781 cttcttcgtg gtgttcccgc ccggtgcgct cgacggcctg ccggcgacct ggatcaccag
      841 cttccacctg ccggccgggc gtcaggcgct gatcgcggag ctggtgcgcg cgcatccgtc
      901 ggtgacggtg ctcgacatcg acgcgctgct ttcgaaggtg cgcgagatca tcgagcgcgt
      961 cgtgctcggc gtcgaatacg tgttcctgtt cacgctggcg gccgggctcg ccgtgctcta
     1021 tgccgcgatc caggcgacgc gcgacgaacg ccgctacgag gccgcgctcg cgcgcacgct
     1081 cggcgcgtcg cggcgcgtcg tgctgcaggg gctgatcgcc gagtttgccg tgctcggcct
     1141 gctcgccggc gtgctggcgg cggccggggc cagcgcgctc ggctggggac tggcgcagca
     1201 ggtgttcgat ttcccgtacc ggcccgatcc gctgctgtgg ctcgccggtg cggcggccgg
     1261 cgtgttcggc gtcgggcttg ccgggctcgc cggcacgcgc gccgtgctcg atcagccgcc
     1321 gctcgcgacg ctgcgcgccg gtgtctgacg cggcgatcgt cgaccagacg atcgtcggcc
     1381 agactctgga caggccggcc gcgtgctcgc ggctgcgccc ctgaccggga gcacgatgat
     1441 ggacactccg agcgaagacc cgcgtgcgcg cctcgaacgg ctcgccagcg aactcgacca
     1501 gcgcctcgcg cgcatcggcg cacgcgaacg ccacgaggac ggcccgccgt cgcaggattt
     1561 cgccgaacag gccaccgagc gcgagaacga tgacgtgatc ggcgcactgg cgctcgacac
     1621 gcgcctgcag ctcgggctgg tgcgcaaggc gctgcggcgc ctcgacgccg gcgactacgg
     1681 cgtctgcgtc gaatgcggca cgccgatccc gtcggcccgg ctcgcggcgc tgccctgggc
     1741 cgagcactgc gtcgcctgtg cggagcgtct ggaaggccgc cacgcctgag nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcacgatcc attcgatgac gtgccaggcg
     1921 cgcggcgtca gcggccacag cgccagcggt cccggtgcgc cgccgaggcc gagcgtgtca
     1981 tggtcgcaga cctccagccc gctgccgccc cacaggtgtt cggcgagggc ggcggcgtcc
     2041 agcccggcag ccgtgtcggg cagcgcgccg tgcggtgcgc gtgccgccga cagcacgtca
     2101 tggcagctcg cccagtgctg tccggccagt gcccggcgaa gccggccggg atcggcgatc
     2161 accggccccg cgacctgctg gccgagcacg ttcgcaaaca gcagcgcggc atcggggtag
     2221 gcccgcgcca gccgcgccgg gccgtcccgg tcacagaggc agtcgagccg gtcgaacacg
     2281 agtttcgtgt ccgcaaaccg gcggccgagc agccggcgag ccagcggatc gggctccagc
     2341 acatgcacct gcccgaagcc ggccagaaac gccgccggca gcgtccagcc cgccgacggg
     2401 ccgatcagca ccagttcgcg gcacggcgga ttccatgcct gcagccagcc ggcgagccgg
     2461 tcggaatacg ctcgccagag cggccggtga cgcagcgcgc gcaggtgata gacgaggccg
     2521 ccggccgggg acagccagcg gtcaacggga aggagcgggg tggttccggg caatggcgtc
     2581 gtgcttcccc cggggcggtt tgcgcccggt tccgtgcctg cgtgcctgcg aaaggcagcg
     2641 aagccgggcg gtcgcgcagt gcgcccccgt ctgaacgacg aaggcggccg cagccgcctc
     2701 cggtggaccg tggcgtgcgg ctttcagtcg ccgagcccct cgaacagctt ggtgctcagg
     2761 taacgctcgc cgaaggacgg gatgatcacg acgatgcgct tgccggcgtt ctcgggcttc
     2821 gccgcctgct gcagcgccgc ccacagtgca gcgcccgagc tgatgccgac cagcaggcct
     2881 tcctcgcgcg cggcgcgccg ggccgtgacc agtgcatcgt cattggtcac gcgcgtgacc
     2941 tcgtcgtaga tgccggtgtt cagcacctcg ggcacgaagc cggcgccgat gccctggatg
     3001 atgtgcgggc ccttctggcc gcccgagagc accggcgagg cgtcgggctc gaccgcgacg
     3061 atcttcaggc ccggacggcg gggtttcagc acctcgccga cgccggtgat cgtgccgccg
     3121 gtgccgatgc cggcgacgaa gatgtcgatc tcgccatcgg tgtcgcgcca gatctcctcg
     3181 gccgtcgtgc gccggtgcac ctcggggttg gccgggttct ggaactgctg cgggatgtag
     3241 tagttcgggt tgccggcggc gagttcctcg gccttgcgga tcgccccggc catgccttcg
     3301 gcgcccggcg tcagcaccag gtcggcaccg tagccgcgca gcagcatgcg ccgttccctg
     3361 ctcatcgtgt ccggcatgac caggcagcac ttgtagccgc gtgccgcgca gaccatcgcc
     3421 agcgcgatac cggtgttgcc gctggtcggt tcgaggatga ccgtgtccgg gccgatcttc
     3481 ccggccgctt cggcggcatc gatcatcgct gcgccgatgc ggtccttgac gctgtgcgcc
     3541 gggttctgga attcgagctt gcagacgacc tcggcgccga ggccggccgt cagccggttc
     3601 agccgcacga gcggggtgtt gccgatcagc tcggtgacgt tgcgggcgat gcgcatggcg
     3661 gatacctcaa ggggtggggg aaggggcggc aggcggccgg ggcgcgcgcg ccggtgccgg
     3721 cggtgcgggc gtcgcaacga acatagccgg cgcgcggcgg cgcggctcag atcacgaagt
     3781 cctcggtcag ggcagcgttc gtggcttcgg gggcgaggaa caccttcatg cgccgcggcc
     3841 gcacccagac cggttcgccg gtggcgtagc ctt
//
LOCUS       DAOQIE010000343         3827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14710_length_3827_cov_7.061506, whole genome shotgun sequence.
ACCESSION   DAOQIE010000343 DAOQIE010000000
VERSION     DAOQIE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_3827_cov_7.061506"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PK144_16405"
     CDS             complement(<1..905)
                     /locus_tag="PK144_16405"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNF67354.1"
                     /translation="MNPDARIQAVTERIRARSRDSRAAYLAEIDAARGRGPLRRTLSC
                     TNLAHGFAACPAGDKLALRSGPQPNIAIVSAYNDLLSAHQPLEAYPAWIKEAAREAGA
                     VAQFAGGVPAMCDGVTQGQDGMELSLFSRDTIALATAVALTHNLFDATLCLGVCDKIV
                     PGLLIGALRFGHLPTIFVPGGPMVSGLSNDAKAEVRKRFAQGLAGRDELLESESQSYH
                     SPGTCTFYGTANSNQMLMEIMGLHLPGSAFVNPGTPLREALTREAARRVARLTALGDE
                     YTPIGHVIDERAIVNGIVGLLATGGS"
     gene            1143..2597
                     /gene="zwf"
                     /locus_tag="PK144_16410"
     CDS             1143..2597
                     /gene="zwf"
                     /locus_tag="PK144_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNF67355.1"
                     /translation="MQVTPPFDLVIFGGNGDLAMRKLLPALYHRHRAGSLCDDGRILA
                     VSRSEMDRATYLNLAREALVAHLGSHFNERDWEKFAQRLDFHRVDANAPGHYSGLAMV
                     LGENTGRVRVFYLSTSPDLFMNICRNLGQAGLVTPESRVVLEKPLGRDLESARAINDA
                     VGEVFEEHQIYRIDHYLGKEAVQNMMALRFGNSVFEPLWRRTWISHVQITVAEQLGVE
                     GRGEFYNGVGAMRDMIQNHLLQLLCIVAMEPPANMDADSVRDEKLRILRSLRPLTGAE
                     VATRSVRGQYRAGAVNGKAVSAFLDEPGIPSDSQTETFVALKAEITNWRWAGTPFYLR
                     TGKRMPEGIAEIALYFRDMPMHLFPTVGGVPNRLVIRLQPDEGLRLDLMAKIPGDDMK
                     LRPVGLNLSFAETFKQRPVQAYERLLGDVIHGRLALFMRRDEQEAAWMWVEPIMQAWV
                     ASGNSPKLYTAGTWGPAAATALISRDGFSWHEDY"
     gene            2600..3310
                     /gene="pgl"
                     /locus_tag="PK144_16415"
     CDS             2600..3310
                     /gene="pgl"
                     /locus_tag="PK144_16415"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNF67356.1"
                     /translation="MADRLDSSAVLHEWPDRDALDRALAEAVGAALQAGLERRGAASL
                     VVSGGRTPLGFLRALAQAPLDWSQVAVTLADERWVDVLDEASNERLLVSALSGTPAAA
                     ARIVGLKTAHADPESALAEVAARLATLPRPFDAVVLGMGDDGHTASLFPDAPQLAATI
                     VSPAPVAALTPRGAAHARLSLTPAALFDAQALFLHITGDGKRSVLDAALGDGPVEALP
                     VRLALRHDRPVCQVYWAP"
     gene            3329..>3827
                     /locus_tag="PK144_16420"
     CDS             3329..>3827
                     /locus_tag="PK144_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF67357.1"
                     /translation="MLLLERLEDLKHHLSRCERKVADLVLGDPQAAIRESLAAFAARA
                     GVSEPTVIRCCRALGCIGWPDFKLRLAQGLASGGGSGHSAVQAGDDAGVVLAKVFDAA
                     ILMLQRTRQAIDAAALEQATRLLAAARRIECYGHGASGIVALDAQQKFYRLGVPVVAY
                     SDAHVH"
BASE COUNT          544 a         1348 c         1358 g          577 t
ORIGIN      
        1 gagccgccgg tcgcgagcag gccgacgatg ccgttgacga tggcgcgctc gtcgatgacg
       61 tggccgatcg gcgtgtattc gtcgccgagc gcggtcaggc gcgcgacccg gcgtgcggct
      121 tcgcgcgtca gcgcctcgcg cagcggcgtg ccggggttga cgaaggcgct gcccggcagg
      181 tgcaggccca tgatctccat cagcatctgg ttgctgttgg cggtgccgta gaaggtgcag
      241 gtgccggggc tgtggtagct ctgcgattcg ctttcgagca gctcgtcacg accggcgagg
      301 ccctgcgcga agcgcttgcg cacctcggcc ttggcgtcgt tcgacaggcc cgagaccatc
      361 ggcccgccgg gcacgaagat cgtcggcaga tgcccgaagc gcagcgcgcc gatcagcagc
      421 cccggcacga tcttgtcgca gacgccgagg cagagcgtgg cgtcgaacag gttgtgcgtc
      481 agcgccaccg cggtggcgag cgcgatggtg tcgcgcgaga acagcgacag ctccatgccg
      541 tcctgcccct gcgtgacgcc gtcgcacatc gccggcacgc cgccggcgaa ctgcgcgacc
      601 gcgccggcct cgcgtgcggc ctccttgatc caggccggat aggcttcgag cggctggtgc
      661 gccgagagca ggtcgttgta ggccgagacg atggcgatgt tcggctgcgg gccgctgcgc
      721 agcgcgagct tgtcgccggc cggacaggca gcgaagccgt gcgcgaggtt ggtgcacgac
      781 agcgtgcggc gcagcggtcc gcgaccgcgc gcggcgtcga tctcggccag gtaggccgcg
      841 cgactgtcgc ggctgcgggc gcggatgcgc tcggtgacgg cctggatgcg ggcatcgggg
      901 ttcatcggcg ggcctccgcg acgggcaatg tccggaatgt agccggacta cggacgatgc
      961 gaaaccggca cgctccggcc gctctgcgca tcgacggcaa cgacagcgat ccatccatcc
     1021 gatcaacagg tttgaccagt gtcccgccag ccggcagcgg cgggcgcgct ttccttcgcc
     1081 ctgtagtcaa actacagttc ccggcgcttc cagttccctg atgcccatgc gaggcggcga
     1141 ccgtgcaggt gactcccccc ttcgatctgg tgatcttcgg cggcaacggc gatctggcga
     1201 tgcgcaagct gctgccggcg ctctatcacc gccaccgcgc cggcagcctc tgcgacgacg
     1261 gccgcatcct cgccgtgtcg cgctcggaga tggatcgcgc cacctatctg aatctggcgc
     1321 gcgaggcgct ggttgcgcac ctcggcagcc atttcaacga gcgcgactgg gagaagttcg
     1381 cgcagcgcct cgacttccac cgcgtcgatg ccaacgcccc cggccactat tcggggctgg
     1441 cgatggtgct cggcgaaaac accggccggg tgcgggtctt ttatctgtcg acctcgccgg
     1501 acctgttcat gaacatctgc cgcaacctcg gccaggccgg gctggtgacg cccgagtcac
     1561 gcgtggtgct ggaaaagccg ctcgggcgcg atctggagtc ggcgcgcgcg atcaacgacg
     1621 cggtcggcga ggtgttcgag gagcaccaga tctaccgcat cgaccattac ctcggtaagg
     1681 aagccgtgca gaacatgatg gcgctgcgct tcggcaactc ggtgttcgag ccgctgtggc
     1741 ggcgcacctg gatcagccac gtgcagatca ccgtcgccga gcagctcggc gtcgagggcc
     1801 gcggcgagtt ttacaacggc gtcggcgcga tgcgcgacat gatccagaac cacctgctgc
     1861 agctgctgtg catcgtcgcg atggagccgc cggcgaacat ggacgccgat tccgtgcgcg
     1921 atgaaaagct gcgcatcctg cgctcgctgc ggccgctgac cggcgccgag gtcgcgacgc
     1981 gcagcgtgcg cggccagtac cgcgccggcg ccgtcaacgg caaggcggtg tcggccttcc
     2041 tcgatgagcc gggcatcccg tccgacagcc agaccgagac cttcgtcgcg ctgaaggccg
     2101 agatcaccaa ctggcgctgg gccggcacgc cgttctacct gcgcaccggc aagcgcatgc
     2161 ccgagggcat cgccgagatc gcgctgtact tccgcgacat gccgatgcac ctgttcccga
     2221 ccgtcggcgg cgtgccgaac cggctggtga tccggctgca gcccgacgag ggcctgcgcc
     2281 tcgacctgat ggcgaagatt ccgggcgacg acatgaagct gcgcccggtc ggcctgaacc
     2341 tgagcttcgc cgagaccttc aagcagcgcc cggtgcaggc ctacgagcgc ctgctcggcg
     2401 acgtgatcca cgggcgtctg gcgctgttca tgcgccgcga cgaacaggaa gccgcgtgga
     2461 tgtgggtcga gccgatcatg caggcctggg tggcgagcgg gaattcaccg aagctctaca
     2521 ccgccggcac ctggggaccg gccgccgcga cggcgctgat cagccgcgac ggcttctcgt
     2581 ggcacgagga ttactgatca tggccgaccg gctggactcg tccgccgtgc tgcacgagtg
     2641 gcccgaccgg gacgcgctcg accgggcgct cgccgaggcc gtgggcgcgg cgctgcaggc
     2701 cggcctcgaa cggcgcggcg cggcgagtct cgtcgtctcc ggcgggcgca cgccgctcgg
     2761 cttcctgcgg gcacttgcgc aggcaccgct cgactggtcg caggtggcgg tgacgctcgc
     2821 cgacgagcgc tgggtcgatg tgctggacga ggccagcaac gagcgcctgc tggtctcggc
     2881 gctctccggc acgccggcgg ccgcagcaag gatcgtcggg ctgaagaccg cacacgccga
     2941 ccccgaatcc gccctggccg aggtcgctgc ccggctcgcg acgctgccgc gccccttcga
     3001 tgccgtcgtg ctcggcatgg gcgatgacgg tcacaccgcc tcgctgttcc cggacgcgcc
     3061 gcagctcgcc gccaccatcg tcagcccggc cccggtcgcc gcgctcactc cgcgtggcgc
     3121 ggcgcatgcg cgcctgtcgc tgacgccggc ggcgctgttc gatgcgcagg cgctgttcct
     3181 gcacatcacc ggcgacggca agcgcagcgt gctcgatgcc gccctcggtg acggcccggt
     3241 cgaggcgctg ccggtgcgcc tcgcactgcg tcatgacagg ccggtctgtc aggtgtattg
     3301 ggcgccgtaa accgctgggc cggccccgat gctgctgctc gaacgcctcg aagacctcaa
     3361 acaccacctg agccgctgcg agcgcaaggt cgccgatctc gtgctcggcg atccgcaggc
     3421 ggcgatccgc gaatcgctcg cggccttcgc cgcgcgcgcc ggcgtcagcg aaccgaccgt
     3481 gatccgctgc tgccgcgcgc ttggctgcat cggctggccg gatttcaagc tgcggctggc
     3541 gcagggcctc gcgagcggcg gcggcagcgg ccacagcgcc gtgcaggccg gcgacgacgc
     3601 cggcgtcgtg ctcgccaagg tcttcgatgc cgcgatcctg atgctgcagc gcacgcgcca
     3661 ggccatcgac gccgccgcgc tcgaacaggc gacgcggctg ctggcggcgg cgcggcgcat
     3721 cgagtgctac ggtcacggcg cctcgggtat cgtggcgctc gacgcgcagc agaagttcta
     3781 ccgcctcggc gtgccggtgg tcgcctacag cgacgcccac gtgcacg
//
LOCUS       DAOQIE010000344         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14719_length_3826_cov_4.833466, whole genome shotgun sequence.
ACCESSION   DAOQIE010000344 DAOQIE010000000
VERSION     DAOQIE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14719_length_3826_cov_4.833466"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1089)
                     /locus_tag="PK144_16425"
     CDS             complement(10..1089)
                     /locus_tag="PK144_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNF67358.1"
                     /translation="MSRRILIVRLSAIGDVVMASGLIPALRARWPDAHLAWLAEPPVR
                     ELLADNPRLDEVIVWPRGEWRQLAREHRYGELARRVRTFVRELRARRFDLALDLQGLL
                     KSAIWTRLSGAPERIAIGPREGAQWLATRAIPRRSEDTRIGSEYRRLALELGAPESGF
                     RLDLAVGPAAHAAAEQALREAFGLDETAPIPPFAVIAPFTTRPQKHWFAERWAALAPQ
                     LTQQSGWPVVMLGGPGDREAAAAIREATPGLIDLSGRTRLAASVAIIAQARLLIGVDT
                     GLTHMGIALGVPTLALFGSTRPYLDTGSPDARVLWSARACSPCRRHPTCGGTFDCLRD
                     HTPASVLAAARTLPALQARLAERAS"
     gene            complement(1086..>1856)
                     /locus_tag="PK144_16430"
     CDS             complement(1086..>1856)
                     /locus_tag="PK144_16430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67359.1"
                     /translation="RLGGFYKLDGYYRHCDAWIGNTQAICDYLIREGLPGSRVFRIGN
                     FVPEAPAVDDGELARLRTGLHLPPTAPVLFALGRFIDKKGFADLLQALARLPAVEAER
                     APRLLLAGDGPQRPALEALVHELRLDGHVHFLGWQPQPAAYFRLADALVCPSRHEPLG
                     NVILEGWSHRRPVLSTANEGASELIRDGDNGLICPLADPDGLAAGIARLLALPAEARE
                     RIAGRGRATLDAEHGQSAVLARYLELYEHLRTLGRRSR"
     assembly_gap    1857..1956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1957..2329)
                     /locus_tag="PK144_16435"
     CDS             complement(<1957..2329)
                     /locus_tag="PK144_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67360.1"
                     /translation="MRTLHVIGSTQMGGADQFFVRLLTALAEAGHDPLAVTRAGSPIA
                     QALAGGPVRQAHLPLANGWDAWSGWQLRRLLGREGFPLVQTYMGRASRLMRVPTASAC
                     VHVARLGGFYKLDGYYRHCDAW"
     gene            2436..3011
                     /locus_tag="PK144_16440"
     CDS             2436..3011
                     /locus_tag="PK144_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF67361.1"
                     /translation="MPATAPASALFLDRDGIVNVDHGYVYRIEDFAFVPGIFALARAA
                     QAAGRRLVIVTNQSGIARGRYDEADYQRLTAWMQARFATEGAPLAGVWHCPHLPGAPA
                     PYGIDCDCRKPAPGLILAARDALGLDLVRSILIGDKARDIESARRAGLGHAWLVSGER
                     AEIDAAAADGVWPSVAALAADARAQAALALQ"
     gene            complement(3005..>3826)
                     /locus_tag="PK144_16445"
     CDS             complement(3005..>3826)
                     /locus_tag="PK144_16445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67362.1"
                     /translation="PHRLQLALGRGLGRLYGRLDRHRRHVVATNLRLCFPALDEAARA
                     RLARAHFEALGMGIFEVASGYWAPRARLAALGEVCGLEHFDAAAARGRGIILLTGHFT
                     TLEIGAGLLALHRSFHPMQRRNENALFNEVMSAERARRTGGACIDRGDLRGMLRVLRR
                     NGAVWYAPDQDYGAQGTVFVPFFGVAARTITATSRLARMSGAAVLPYWPERLADGRYR
                     VHILPMLEDFPSADETADAARINRLIEDWARCVPEQYYWVHRRFKGRPAGEPPVY"
BASE COUNT          516 a         1367 c         1371 g          472 t
ORIGIN      
        1 gcacgacctt cacgacgcgc gctccgccag ccgtgcctgc agcgccggca gcgtgcgcgc
       61 cgcggccagc accgaggccg gcgtgtggtc gcgcaggcag tcgaatgtgc cgccgcaggt
      121 cgggtgccgc cggcacggcg agcaggcgcg cgcgctccag agcacgcgcg catccggcga
      181 gccggtgtcg agatacggcc gcgtcgagcc gaacagcgcc agcgtcggca cgccgagcgc
      241 gatgcccatg tgcgtgaggc cggtgtcgac gccgatcagc agccgtgcct gcgcgatgat
      301 cgccacactc gccgcgagcc gcgtgcggcc gctgaggtcg atgagcccgg gcgtcgcctc
      361 gcggatcgct gcggccgcct cgcgatcgcc ggggccgccg agcatcacga ccggccagcc
      421 ggactgctgc gtgagctgcg gcgcgagtgc ggcccagcgc tcggcgaacc agtgcttctg
      481 cggccgggtc gtgaacggcg caatgaccgc gaacggcggg atcggcgccg tttcatcaag
      541 tccgaaggct tcgcgcaggg cctgttccgc agcggcgtgg gcggcggggc cgaccgcgag
      601 atcgagccgg aaaccggact ccggcgcgcc gagttcgagc gcgagccgcc ggtattcgga
      661 gccgatgcgg gtgtcctcgc tgcggcgcgg gatcgcgcgc gtcgccagcc actgcgcgcc
      721 ctcgcgcggg ccgatggcga tgcgctcggg cgcgccggag agccgcgtcc agatcgcgct
      781 cttcagcagc ccctgcaggt cgagcgcgag atcgaagcgc cgtgcgcgca gctcgcgcac
      841 gaaggtccgc acgcgtcgcg ccagttcgcc gtagcggtgt tcgcgtgcga gctgccgcca
      901 ctcgccgcgc ggccagacga tcacctcgtc gaggcgcggg ttgtcggcga gcagctcgcg
      961 caccggcggt tcggcgagcc aggcgaggtg cgcatccggc cagcgcgcgc gcagcgccgg
     1021 gatcaggccc gaggccatca ccacgtcgcc gatcgccgac aggcgcacga tcaggatgcg
     1081 ccggctcatc gcgaccgccg tccgagtgtg cgcaggtgct cgtagagctc cagatagcgc
     1141 gcgagcacgg cgctctggcc gtgttcggca tcgagcgttg cgcgtccgcg tccggcgatc
     1201 cgctcgcgcg cttcggccgg cagcgccagc agccgggcga tgccggcggc gaggccgtcc
     1261 ggatcggcga gcgggcagat caggccgttg tcgccgtcgc ggatcagctc gctcgcgccc
     1321 tcgttggcgg tcgagagcac cggccgccgg tggctccagc cttcgaggat gacgttgccg
     1381 agcggctcgt gccgcgacgg gcagaccagc gcatcggcaa gccggaagta cgccgccggc
     1441 tgcggctgcc agccgagaaa atgcacgtgg ccgtcgaggc gcagctcgtg cacgagggct
     1501 tcgagcgccg ggcgctgcgg gccgtcgccg gcgagcagca gccggggcgc gcgctcggcc
     1561 tcgaccgccg gcagccgcgc cagcgcctgc agcaggtcgg cgaagccctt cttgtcgatg
     1621 aagcgcccga gcgcgaacag caccggcgcc gtcggcggca ggtgcagccc ggtgcgcagg
     1681 cgcgcgagct cgccgtcgtc caccgccggc gcttcgggca cgaagttgcc gatccggaac
     1741 acgcgcgagc cgggcaggcc ctcgcggatc aggtagtcgc agatcgcctg cgtgttgccg
     1801 atccacgcat cgcagtggcg gtaatagcca tcgagcttgt agaagccgcc gaggcgnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcca cgcatcgcag tggcggtaat
     1981 agccatcgag cttgtagaag ccgccgaggc gtgcgacgtg tacgcaggcg ctggcggtcg
     2041 gtacgcgcat cagccgcgac gcgcggccca tgtaggtttg cacgagcggg aagccctcgc
     2101 gcccgagcag ccgccgcaac tgccacccgc tccacgcgtc ccagccgttg gcaagcggca
     2161 ggtgtgcctg ccggaccgga ccgccggcga gcgcctgcgc gatcggcgag ccggcgcgcg
     2221 tgacggccag cgggtcgtgg ccagcctcgg cgagtgccgt cagcaggcgc acgaagaact
     2281 ggtcggcacc gcccatctgc gtcgaaccga tgacgtgcag cgtgcgcatg cgcgggaaca
     2341 ctccggtccg ggccgtgagg aaagggccgg caaggatacc ggctcgccgc gccggccggt
     2401 ataagacgct ccctgcctgt cgcgagccgc cgccgatgcc cgccaccgct cccgcttctg
     2461 cgctgttcct cgaccgcgac ggcatcgtca acgtcgatca cggctacgtg taccgcatcg
     2521 aggacttcgc gttcgtgccc ggcatcttcg cgctggcgcg cgctgcgcag gctgccggcc
     2581 ggcggctcgt catcgtcacc aaccagtccg gcatcgcccg cggccgctat gacgaggccg
     2641 actaccagcg gctgacggca tggatgcagg cgcgtttcgc caccgagggc gcgccgctcg
     2701 ccggcgtctg gcactgcccg cacctgcccg gcgcgccggc gccatatggc atcgactgcg
     2761 actgccgcaa gccggcgccg gggctgatcc tcgccgcccg cgacgcgctc gggctggatc
     2821 tggtgcgctc gatcctgatc ggcgacaagg cccgcgacat cgaatcggcg cgccgtgccg
     2881 gcctcggcca cgcctggctg gtcagcggcg agcgtgccga gatcgacgcg gccgcggccg
     2941 atggcgtctg gccgagcgtc gcggcgctcg ccgctgacgc ccgggcgcag gccgcactcg
     3001 cgcttcagta gaccggcggc tcgccggccg gccggccctt gaagcgccgg tgtacccagt
     3061 agtactgctc gggcacgcag cgtgcccagt cctcgatcag ccggttgatg cgcgcggcat
     3121 cggcggtctc gtcagcggac gggaagtctt ccagcatcgg caggatgtgc acgcggtagc
     3181 gcccgtccgc cagacgctcg ggccagtacg gcagcaccgc cgcaccgctc atgcgcgcca
     3241 ggcgcgaggt cgccgtgatc gtgcgcgccg ccacgccgaa gaacggcacg aacaccgtgc
     3301 cctgcgctcc gtagtcctga tccggcgcgt accagaccgc gccgttgcgc ctgagcacgc
     3361 gcagcatgcc gcgcagatca ccgcggtcga tgcaggcgcc gccggtgcgg cgtgcgcgtt
     3421 cggcgctcat gacttcgttg aacagcgcgt tctcgttgcg acgctgcatc ggatgaaacg
     3481 accggtgcag cgccagcagg ccggcaccga tctccagcgt cgtgaagtgt ccggtcagca
     3541 ggatgatgcc gcgcccgcgc gcggcggccg catcgaaatg ttcgagtccg cagacctcgc
     3601 cgagcgcggc cagccgcgcg cgcggcgccc agtagccgct cgcgacctcg aagatgccca
     3661 tgccgagcgc ctcgaaatgc gcacgggcga ggcgcgcgcg cgcggcttca tcgagcgccg
     3721 ggaagcacag ccgcaggttg gtcgcaacca cgtgccggcg atggcggtcg agccgcccgt
     3781 acagccggcc gaggccgcgc ccgagcgcaa gctgcagccg gtgcgg
//
LOCUS       DAOQIE010000345         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_14866_length_3799_cov_6.332265, whole genome shotgun sequence.
ACCESSION   DAOQIE010000345 DAOQIE010000000
VERSION     DAOQIE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14866_length_3799_cov_6.332265"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PK144_16450"
     CDS             <1..902
                     /locus_tag="PK144_16450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67363.1"
                     /translation="YLRTQRPLVLMSAKGKDDLLALDARDAAKNAAGGTRVFLRCGIH
                     LSSRPKMVSRNPLRGLWHRWRLRRLYARPDGVICVSEGVADDLAQLTGFARARIAVIR
                     NPTISAGFDARLAEPLDDPWFAPGAPPVILGAGSLAPVKHFEVLIEAAAALMRTRELR
                     LVILGEGKERARLEALADSLGIRERVRLPGFVGNVLPWMRRAAVFVLSSEREGSPNVV
                     TEALACGTPVVATDCPSGPREILDGGRFGPLIPIGDAAAMQRAIAAVLDAPLPAATLQ
                     SAIAEYTLPRACAGYLRAFGLAG"
     gene            899..2044
                     /locus_tag="PK144_16455"
     CDS             899..2044
                     /locus_tag="PK144_16455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67364.1"
                     /translation="MRPRPDLALFAATSGHSGVDRIVANLLPALAGLGLRIDLLNIRG
                     HGPRLDPVPDGVRVVDLGRAHVWPALPALVAYLKRERPRVLLSDKDKVNRAALAARAL
                     AGGDTRAFVRLGTTVSHNLASRPWFDRAVQTLSMRSLYRFAAGVLVPSQGVAGDFAAF
                     TGLPRARIHVVPNPVVTPHMQALAAGAPGHPWLTDGGAPVILGVGELSARKDFATLLR
                     AFARLRRQRPCRLLILGEGRRRERLLALAAELGIADDLALPGFVANPYPAMRAAAAFA
                     LTSQWEGLGIVLVEALALGTPVVACDCPSGPREVLADGRYGPLVAVGDDAALAAALAG
                     LLDVPPPPALLAEAAAPYTVEASARAYARALGLELPACSPAPQENPA"
     gene            2041..3156
                     /locus_tag="PK144_16460"
     CDS             2041..3156
                     /locus_tag="PK144_16460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67365.1"
                     /translation="MTRIAVLISFSGAGGVERMVLNLIDGFATHGVAVDLLAIRAESA
                     HLGALPEGVRMIDLGTRHSTLAIPALARYLAAERPAALLAAKDRAIRAAVLARALARV
                     PCRIVGRLGTNLSAALEGKAGLQRWLRTAPMRLLYRPVEHVVAVSQGVLDDTRRLTGL
                     PAARLSVIRNPVVTPRLATLAAESPPHPWTEASEPPLILAAGRLTEQKDFPTLLRAFA
                     TVRAARPARLAILGEGRGRAALTALAQELGIADALLLPGFAPNPYAWMARARLFVLSS
                     AWEGSPNVLTEALALGVPSVATDCPSGPREVLAGGRFGPLVPVGDPAQLAQAMLATLA
                     APLAPDTLRAAVADYTQAAAAAAYLRVLLPQRQPERP"
     gene            3283..>3799
                     /locus_tag="PK144_16465"
     CDS             3283..>3799
                     /locus_tag="PK144_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNF67366.1"
                     /translation="MLLSLKYNFLFVHTAKTGGTSVRDALRPLRLRDPWFYAQFLCSR
                     LSGLSGHRLGIKFPRHSKIIAAQEMLPHELFEKLFKFVFVRNPWDLQVSSFHHIRRER
                     PHLMAHVETFEQFMRWKLDPERPYQYHVDTSIELQSDYCIDLHGKVLVDFIGRTENLQ
                     PDFEEACRRIGI"
BASE COUNT          450 a         1417 c         1357 g          575 t
ORIGIN      
        1 actacctgcg cacgcagcgc ccgctggtgc tgatgtcggc caagggcaag gacgatttgc
       61 tcgcgctcga tgcccgcgac gcggcgaaaa acgcggcagg cggcacgcgc gtgttcctgc
      121 gctgcggcat tcatctgtcc tcgcgcccga agatggtctc gcgcaacccg ctgcgcgggc
      181 tctggcaccg ctggcggctg cgccggctgt acgcgcgccc cgacggcgtg atctgcgtgt
      241 cggagggcgt cgccgatgac ctcgcgcagc tgaccggctt tgcgcgcgcg cgcatcgcgg
      301 tcatccgcaa cccgaccatc agcgccggct tcgatgcgcg cctcgccgag ccgctcgacg
      361 atccgtggtt cgcgcccggc gcgccgccgg tgatcctcgg cgccggcagc ctggcgccgg
      421 tcaagcactt cgaggtgctg atcgaggccg ccgcggcgct gatgcgcacg cgcgagctgc
      481 gcctcgtgat cctcggcgaa ggcaaggagc gcgcacggct cgaagccctg gccgactcgc
      541 tcggcatccg cgagcgcgtg cgcctgcccg gcttcgtcgg caacgtgctg ccgtggatgc
      601 gccgcgccgc ggtgttcgtg ctgtcgtccg agcgcgaagg ctcgccgaac gtcgtcaccg
      661 aggcgctggc ctgcggcacg ccggtggtcg cgaccgactg cccgagcggg ccgcgcgaga
      721 ttctcgatgg cggccgcttc gggccgctga tcccgatcgg cgatgccgca gcgatgcagc
      781 gcgcgatcgc cgccgtgctc gatgcgccgc tgccggccgc aaccctgcag tccgccatcg
      841 ccgaatacac cttgccgcgc gcctgcgccg gctatctgcg cgccttcgga ctggccggat
      901 gaggccgcgc ccggatctcg cgctgttcgc ggcgacctcg ggccacagcg gcgtcgatcg
      961 catcgtcgcc aacctgctgc cggcgctcgc cggcctgggc ctgcgcatcg accttctgaa
     1021 catccgcggc cacggcccgc gcctcgatcc ggtgccggac ggcgtgcgcg tcgtcgatct
     1081 cggccgcgcc cacgtctggc cggcgctgcc ggcgctggtc gcttatctga agcgcgagcg
     1141 cccgcgcgtg ctgctgtcgg acaaggacaa ggtcaaccgt gccgcgctcg ccgcccgggc
     1201 gctcgccggg ggcgacacgc gcgcgttcgt gcgcctcggc acgacggtct cgcacaacct
     1261 cgccagccgc ccgtggttcg atcgcgcggt gcagacgctg tcgatgcgca gcctctaccg
     1321 cttcgccgcc ggcgtgctcg tgccctcgca aggcgtcgcc ggggacttcg cggccttcac
     1381 cggcctgccg cgcgcgcgca tccacgtcgt gccgaacccg gtagtcacgc cgcacatgca
     1441 ggcgctcgcc gccggcgcgc cggggcatcc ctggctcacg gacggcggcg cgccggtgat
     1501 cctcggcgtc ggcgagctgt cggcgcgcaa ggacttcgcg acgctgctgc gggccttcgc
     1561 gcggctgcgc cgccagcgtc cgtgccggct gctgatcctc ggcgaaggcc gccggcgcga
     1621 acggctgctc gcgctcgccg ccgaactcgg catcgccgat gacctcgcgc tgcccggctt
     1681 cgtcgccaat ccctacccgg cgatgcgcgc cgccgcggcc ttcgcgctga cctcgcagtg
     1741 ggaaggactc ggcatcgtgc tggtcgaggc gctcgcgctc ggcacgccgg tcgtcgcctg
     1801 cgactgcccg agcggcccgc gcgaggtgct cgccgatggt cgctacggcc cgctggtggc
     1861 ggtcggcgac gatgccgcgc tcgctgcggc cctcgccggc ctgctcgatg tcccgccccc
     1921 gccggcgctg ctcgccgaag ccgcggcacc gtacacggtc gaggccagcg ccagggcgta
     1981 tgcgcgggcg ctggggctgg agctgccggc atgcagtccg gcgccgcagg agaaccccgc
     2041 atgacccgca tcgcggtgct gatctcgttt tccggggccg ggggcgtcga gcgcatggtg
     2101 ctgaacctga tcgacggttt cgcgacgcac ggcgtcgcgg tcgatctgct ggcgatccgt
     2161 gccgagagcg cgcacctcgg cgcgctgccg gagggcgtgc gcatgatcga tctcggcacg
     2221 cgccacagca cgctggcgat cccggcgctg gcgcgttacc tcgcggccga aaggccggcg
     2281 gcgctgctcg cggccaagga ccgcgcgatc cgcgccgcgg tcctcgcccg cgcgctcgcc
     2341 cgcgtgccgt gccggatcgt cgggcggctc ggcaccaatc tgtcggcggc gctcgaaggc
     2401 aaggccgggc tgcagcgctg gctgcgcacg gcgccgatgc ggctgctgta ccggccggtc
     2461 gagcatgtgg tcgcggtttc gcagggcgtg ctcgacgaca cgcgccggct gaccggcctg
     2521 ccggcggcgc ggctgtcggt gatccgcaac ccggtcgtga cgccgcggct cgcgaccctc
     2581 gcggccgaat cccccccgca tccgtggacg gaggcaagcg agccgccgct gatcctcgcc
     2641 gccggccggc tgaccgagca gaaggatttc ccgacgctgc tgcgtgcgtt cgcgacggtg
     2701 cgcgccgcgc gcccggcgcg gctcgcgatc ctcggcgaag gccgcgggcg cgcggcgctg
     2761 acggcgctgg cgcaggaact cggcattgcc gatgcgctgc tgctgccggg cttcgcgccg
     2821 aatccgtatg cgtggatggc gcgcgcgcgg ctgttcgtgc tgtcctcggc ctgggagggt
     2881 tcgccgaacg tgctgaccga ggcgctggcg ctcggcgtgc cgtcggtcgc caccgactgc
     2941 ccgagcggtc cgcgcgaggt gctggccggc gggcgcttcg ggccgctggt gccggtcggc
     3001 gatcccgcgc aactggcgca ggcgatgctg gcgacgctgg ccgcaccgct cgcgcccgac
     3061 accttgcgcg cggcggtcgc cgactacacg caggcggcgg cggcggcggc gtatctgcgc
     3121 gtgctgctgc cgcaacggca gccggagcgg ccgtgaacgc cgggcgaggc ggcggccgtt
     3181 caaaccagac gatctgaaaa ttctactttt aatgctttcc tttcacgccg ggtccgcacc
     3241 gaccatggcg ccatcccgat ccccgtgcgc ggaggcgccg aaatgctgct gtcgctgaag
     3301 tacaacttcc tgttcgtaca caccgccaag accggcggca cctcggtgcg cgatgcgctg
     3361 cggccgctgc ggctgcgcga tccgtggttc tacgcgcagt tcctgtgctc gcggctgtcg
     3421 gggctgagcg ggcaccggct cggcatcaag tttccgcggc attcgaagat catcgcggcg
     3481 caggagatgc tgccgcacga gctgttcgag aagctgttca agttcgtgtt cgtgcgcaat
     3541 ccgtgggatc tgcaggtgag ttccttccac cacatccgcc gcgaacgccc gcacctgatg
     3601 gcgcacgtcg agaccttcga gcagttcatg cgctggaagc tcgacccgga gcgcccgtac
     3661 cagtaccacg tcgataccag catcgagctg cagtcggatt actgcatcga cctgcacggc
     3721 aaggtgctgg tggatttcat cggccgcacc gaaaacctgc agcccgactt cgaggaagcc
     3781 tgccgccgca tcggcatcc
//
LOCUS       DAOQIE010000346         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15177_length_3750_cov_6.994587, whole genome shotgun sequence.
ACCESSION   DAOQIE010000346 DAOQIE010000000
VERSION     DAOQIE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15177_length_3750_cov_6.994587"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK144_16470"
     CDS             complement(<1..251)
                     /locus_tag="PK144_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNF67367.1"
                     /translation="MNATRTVPAALAVLELTVNNHPGVMSHVCGLFARRACNVEGIIC
                     LPLGDGARSRIWLQVPDTDKLEPMTRQARKLEDVLEVIR"
     gene            complement(255..1922)
                     /gene="ilvB"
                     /locus_tag="PK144_16475"
     CDS             complement(255..1922)
                     /gene="ilvB"
                     /locus_tag="PK144_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HNF67368.1"
                     /translation="MKMTGAELIIRLLERQGITTVAGIPGGAILPLYDALGASTQIRH
                     VLTRHEQGAGFIAQGMARASGQAAVCLASSGPGATNLITAIADARLDSIPVVCITGQV
                     PSAMIGTDAFQEVDTYGLTVPITKHNFLVDSVEELLDVVPRAFRIALSGRPGPVLIDV
                     PKDIQNATIEFDEWPQPGCAEPAPQPADTALAAALALIAQAQRPILYLGGGVVHAGAA
                     PLARELAETAGLPTVMTLMALGTLPETHLLQLGMLGMHGARCTNLALAECDLLIAVGA
                     RFDDRATGKVAEFCPNAAIVHIDIDASELDKIKTAHVALNGDVREVLGRLLPRLGAQA
                     DRRAWLARIGELKRDFPLKLDGRHNPRHHYGLIRATARAAGPAAIVTTDVGQHQMWAA
                     QAYPLDRPRQWLTSGGLGTMGFGLPAAIGAALAEPQRPVVCFSGDGSILMNIQELATL
                     AEEDLDVKIVLFNNGALGLVHQQQTLFYGGRLNASVFRGQPDFVRLAQAFGIRTIDLD
                     AEPDVPDALAAAFAERGPCLIHATIGLEEKVYPMVPPGAANTDMIGG"
     gene            complement(2286..2696)
                     /locus_tag="PK144_16480"
     CDS             complement(2286..2696)
                     /locus_tag="PK144_16480"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HNF67369.1"
                     /translation="MKPAQLARFERKARLLARSEAGRSALAEAGDDWLAHTATVDRAV
                     TFVAEQRTPILLGAGALLLYLARGKPKPRRRPGREDRAGRDEPSAAGWLARLMIVLRL
                     ARTLQTVARHLPRPHPRAVASVSRPRPDAPRGLG"
     gene            complement(2693..3079)
                     /locus_tag="PK144_16485"
     CDS             complement(2693..3079)
                     /locus_tag="PK144_16485"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNF67370.1"
                     /translation="MRAGPAASVYSAGRGVLTALLGLIETRLALFALELEEERERLVQ
                     IGIVAGIALMCAALAIGCTALFVIVLFWDTHRLLAIAGVGGAGLLGLGLCLLWIARRG
                     RHPLLIATRTELAADLDRLRGRGPQP"
     gene            complement(3083..3406)
                     /locus_tag="PK144_16490"
     CDS             complement(3083..3406)
                     /locus_tag="PK144_16490"
                     /inference="COORDINATES: protein motif:HMM:NF039524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNF67371.1"
                     /translation="MSIKDSQTAQSADRVADEWNNLIAALEGLAAAAAADGSERYAEV
                     RQRAEAALASARARAEANARGAQDRVHDLASQADVYVRQNPWQAIGIGAGIGLIAGLL
                     LSRKG"
BASE COUNT          533 a         1254 c         1423 g          540 t
ORIGIN      
        1 tggcggatga cttcgagcac gtcttcgagc ttgcgtgcct gacgggtcat cggttcgagc
       61 ttgtcggtgt ccggcacctg cagccagatg cggctgcggg cgccgtcgcc gagcggcagg
      121 cagatgatgc cttcgacgtt gcaggcgcgg cgggcgaaca ggccgcagac gtgcgacatg
      181 acgccggggt ggttgttgac ggtgagttcg agcacggcca gcgcggcggg cacggtacgg
      241 gtggcattca tggttcagcc tccgatcatg tcggtgttgg cggcgcccgg cggcaccatc
      301 gggtagacct tttcctccag cccgatcgtc gcgtggatca ggcagggacc gcgttcggcg
      361 aaggccgcgg cgagcgcatc gggcacgtcc ggttcggcgt cgagatcgat ggtgcggatg
      421 ccgaaggcct gggcgaggcg cacgaaatcg ggctggccgc ggaacaccga ggcgttcagc
      481 cggccgccgt agaacagcgt ctgctgctga tgcacgaggc cgagcgcgcc gttgttgaac
      541 agcacgatct tcacgtcgag gtcctcctcg gcgagcgtcg cgagttcctg gatgttcatc
      601 aggatcgagc cgtcgccgct gaagcacacg accgggcgct gcggttcggc gagcgcggca
      661 ccgatcgccg ccggcagccc gaagcccatg gtcccgagtc cgcccgaggt cagccactgg
      721 cgcgggcggt cgagcggata agcctgcgcg gcccacatct ggtgctggcc gacatcggtg
      781 gtgacgatcg cggccgggcc ggcggcgcgt gccgtcgcgc ggatcaggcc gtagtggtgg
      841 cgcgggttgt ggcgaccgtc gagcttcagc gggaagtcgc gcttcagctc gccgatgcgc
      901 gccagccacg cacggcgatc cgcctgcgcg ccgagccgcg gcagcagccg gccgagcacc
      961 tcgcgcacgt cgccgttgag cgccacgtgc gcggtcttga tcttgtcgag ttcgctcgcg
     1021 tcgatgtcga tatgcacgat cgccgcgttc gggcagaact cggcgacctt gccggtggcg
     1081 cggtcatcga agcgcgcgcc gaccgcgatc agcaggtcgc attcggcgag cgcgagattc
     1141 gtgcagcgcg cgccgtgcat gccgagcatg ccgagctgca gcaagtgcgt ttccggcagc
     1201 gtgccgagcg ccatcagcgt catcacggtc ggcaggccgg cggtttcggc gagttcgcgc
     1261 gccagcggcg cggcgccggc atgcacgacg ccgccgccga ggtacaggat cgggcgctgc
     1321 gcctgcgcga tcagcgccag cgcggccgcg agcgcggtgt cggccggctg cggggccggc
     1381 tcggcgcagc ccggctgcgg ccattcgtcg aactcgatgg tcgcgttctg gatgtccttc
     1441 ggcacgtcga tcagcaccgg ccccgggcgg ccggacagcg cgatgcggaa ggcccgcggc
     1501 accacgtcga gcagttcctc gacgctatcg acgaggaagt tgtgcttggt gatcggcacg
     1561 gtcaggccgt aggtgtcgac ctcctggaag gcatcggtgc cgatcatcgc gctcggcacc
     1621 tgaccggtga tgcagaccac cgggatcgag tcgaggcggg catcggcgat cgccgtgatc
     1681 aggttggtcg cgcccggacc gctggaggcc aggcacacgg ccgcctgacc gctggcgcgc
     1741 gccatgccct gcgcgatgaa gccggcgccc tgctcatggc gggtcagcac gtggcggatc
     1801 tgcgtcgagg cgccgagcgc gtcgtacagc ggcaggatcg cgccgcccgg gatgccggcg
     1861 acggtcgtga tgccctgtcg ttccagcagg cggatgatga gttcggcgcc cgtcatcttc
     1921 atggtctgcg tactccgtcg tcacaagtgg cgttgcgggg gctcggggcc ggccgggacg
     1981 gggaggacgc agaaacgaga acccccgccg gcctgtcgcg gccggcgggg gttctggggt
     2041 tcggaggaaa ctgcctcgac gctcagaccc gggcggacgc gaccgcgtgc gctaccagta
     2101 ccaggcttac gcgtacgacg acggccgcga gaaggctttc gcggtcgtga cgggcgtggg
     2161 caggcaggcg ggcggcggtc atggctcggg tgtttcgcaa ggcggtggcc gtttcgggac
     2221 gcgcactgta gccgcgacgg cgggcgcgag gcaagccgga aatcggcgcg gccggccttg
     2281 gggtttcagc ccaggccgcg cggcgcatcg ggccgcggtc gcgacacgct ggcgacggcg
     2341 cgcggatgcg ggcgcggcag gtggcgggcg accgtctgca gcgtacgcgc gaggcgcagc
     2401 acgatcatca gccgcgccag ccagccggcg gccgagggtt cgtcacgacc ggcccgatcc
     2461 tcgcggcccg ggcgccggcg cggtttcggc ttgccgcgcg ccagatacag cagcagggcg
     2521 ccggcgccga gcaggatcgg cgtgcgctgc tcggcgacga aggtcacggc ccggtcgacg
     2581 gtcgccgtgt gcgccagcca gtcgtcgccg gcttctgcca gcgccgaacg gccggcctcg
     2641 gagcgcgcca gcagccgggc cttgcgctcg aaacgggcga gctgcgccgg cttcatggct
     2701 gcggcccgcg cccgcgcagg cggtcgaggt cggccgcgag ctcggtgcgc gtcgcgatca
     2761 gcagcgggtg acggccgcgc ctggcgatcc agagcaggca caggcccaga ccgagcagcc
     2821 cggcgccccc gaccccggcg atcgcaagca gccggtgcgt gtcccagaac agcacgatca
     2881 cgaacagcgc cgtgcagccg atcgccagcg ccgcgcacat cagcgcgatg ccggcaacga
     2941 tgccgatctg cacgaggcgc tcgcgctcct cttcgagctc gagcgcgaac agcgccagtc
     3001 gggtctcgat cagcccgagc agcgcggtca gcacgccgcg accggcgctg tacaccgagg
     3061 cggcgggccc ggcgcgcatg cgtcagccct tgcggctcag cagcaggccg gcgatcaaac
     3121 cgatgccggc gccgatgccg atcgcctgcc aggggttctg gcgcacgtag acatcggcct
     3181 ggctggcgag gtcatggacg cgatcctgcg cgccgcgggc gttggcctcg gcccgcgcgc
     3241 gcgccgaggc gagcgcggcc tcggcgcgct ggcggacctc ggcgtagcgc tcgctgccat
     3301 cggcagcggc ggccgcggcg agcccttcga gcgcagcgat caggttgttc cattcatcgg
     3361 cgacgcggtc ggcggactgg gcggtttgtg aatccttgat ggacatgggc gtgacctcaa
     3421 cgggcaaggg ggccgggctg tccggcgccg tggtggcgcg gcatcggccg tggttaatgc
     3481 gacagcgtag aaagggaatc gggcagtgca agccggcgct tcgacatttg cgtgcatcct
     3541 ttcaggaaac acgtccgaag cacgctccgg tttcgtggtt ccgcagccac cggatgctta
     3601 ttcaattaca cagccggtca cggaaattcc gtaaccggct gcaaggcatt acgaggtcag
     3661 tgtccggtcg aaaccggtct gcggatcgga cccgacggcg gccggatcgt tccgcggatc
     3721 gccactgacg gcctggagcg cggctcaggg
//
LOCUS       DAOQIE010000347         3726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15361_length_3726_cov_8.628984, whole genome shotgun sequence.
ACCESSION   DAOQIE010000347 DAOQIE010000000
VERSION     DAOQIE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15361_length_3726_cov_8.628984"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..2585
                     /locus_tag="PK144_16495"
     CDS             108..2585
                     /locus_tag="PK144_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNF67372.1"
                     /translation="MSEGDSVGNGNGNGAAAKVPQWTDEDDKALHEAARYLSTAHLSF
                     IAISIYLALAIWQTTHQQLLLGASFTLPVLNVSIDLVTFYTLGPWLYVLLHATLLTQL
                     RLFARSVFRWGLARQKNRDTELPLGLSPLLYYLLLQRMALSRLMLATAVGTTLLIGPL
                     LLVWLFQVRFLAYHDGTVTMWQRGAVFADGALLLLMWPQVVSRRIALGVTRGSSRGRL
                     RRFLRAWLCGFHDERIGCVEQSKTLDRARRRTLRTLMLSIVLPMPLLLGLSWLFRDAG
                     DADGLLLSYAACCLAGLALAYWQVRRPAGGINHEDVQPRSPFVLSYAAIIGPAAVCSL
                     SLFTLVPTIAPDACIRSSDQPRCRWLAVASGASWLEPVLLYSLAAISGYRLLLPGQID
                     ELNHVLLDPPDGDSAEALRWRPGWCAWLDQPKPRSPDERTHDSAPPPPPRRCGRLGPV
                     ALPTALLFEPDLQRGNAGDTRRRLLPSRYLHLRGAALDMDIKPEDVALEKKADGAPLT
                     CDRDARRDNEPTDPGEKRVWQYARALRVQFRDLAGADLRDASLPLGQFQTVNLYGAML
                     DGAYLNGARLYGIDLRYASLTWSHLDNSDWDGVQADFSALDHADLRCARILRTDLVGA
                     RMIEVETARLHLEHSDLRGALIVSQPPRPDSDPRPAPENRSVLAGLTATRGSAAELLK
                     ERALRRCPPGASSVQSVPAKLPSPPPLFGIDDTQGPAAAGRQAAQMTCTLQEVQPEDF
                     REPPPRRLPTRYANALVLMAPPFAAQGFEQSTEWLEAVNQVFTQQPVCPAAEDALLRA
                     RLAFALQPKATPAAPTAAQRCEPRPAD"
     gene            2659..3714
                     /locus_tag="PK144_16500"
     CDS             2659..3714
                     /locus_tag="PK144_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67373.1"
                     /translation="MSERYSIIRPTPLGRAELERVAATLPDLITATGPDGELRLSVQR
                     AGGLVQPIDWQYGTLSVDLPDADVLAVLIELAARLCARVRDDSLMTWRTPFDSYVHAD
                     DTEAVRAQRQRLDALRRRRRLRAWLRAGLIAMTGIVVLLISHPAFWARPLTDPGSRFA
                     LPPDWRAGFTGGRPDVLLLPADDFSEAYAAHLGERLHELTGLSVRTTLAVGLGRVQLF
                     PDSPQYDAQTLVETATPAIDRLRAQYGAVPVILLTQRDINARERSTRFSFAQHYPGWR
                     VSVVSSARLLPGTLIGRASDATLETRLLKFLLRSVGQQVYRLPRDTDIDSVMYAPIMS
                     LSDLDRMGMKLARPGAP"
BASE COUNT          521 a         1403 c         1246 g          556 t
ORIGIN      
        1 cgcgcccatc caccggcatg aacagatcgt aagtcgcaat ccgcccgcat ccggctttgc
       61 tcagtggctg tgacgaagtc ctgacactga tgttgcagga ggcggtgatg agcgaaggcg
      121 acagcgtcgg caacggcaac ggcaacgggg ctgcggccaa agtgccgcag tggacggacg
      181 aggacgacaa ggccctgcac gaggccgcga gatacctcag taccgcgcac ctgagcttca
      241 tcgccatcag catctatctg gcactggcga tctggcagac cacgcaccag caactgctgc
      301 tcggcgccag cttcacgctg ccggtgctga acgtctcgat cgacctcgtc accttctaca
      361 cgctcggccc gtggctgtac gtgctgctgc acgcgacgct gctgacgcag ctgcggctgt
      421 tcgcccgcag cgtctttcgc tgggggctgg cccggcagaa gaaccgcgat accgaactgc
      481 cgctcgggct gtcgccgctg ctctattacc tgctgctgca gcgcatggcg ctgtcacggc
      541 tgatgctcgc gaccgcggtc ggcaccacgc tgctgatcgg gccgctgctg ctggtgtggc
      601 tgttccaggt gcgcttcctc gcctaccacg acggcaccgt gaccatgtgg cagcgcggcg
      661 cggtgttcgc cgatggcgca ctcctgctgc tgatgtggcc gcaggtggtg tcgcggcgca
      721 tcgcgctggg ggtgacccgc ggcagcagcc gcggccggct gcgccgcttc ctgcgcgcct
      781 ggctctgcgg cttccatgac gagcgcatcg gctgcgtcga acagtcgaaa accctggacc
      841 gggcccgccg gcgcacgctg cgcacgctga tgctgtcgat cgtgctgccg atgccgctgc
      901 tgctcgggct gtcctggctg ttccgcgatg ccggcgatgc cgacggcctg ctgctcagct
      961 acgccgcctg ctgcctggcc gggctcgcgc ttgcgtactg gcaggtacgc cgcccggccg
     1021 gtggcatcaa ccacgaggac gtgcagccac gctcgccgtt cgtgctgtcg tatgcggcca
     1081 tcatcggccc ggcggcggtc tgctcgctgt cgctgttcac gctggtgccg accatcgccc
     1141 ctgacgcctg catccgctct tccgaccagc ccagatgccg atggctggcc gtggcgagcg
     1201 gcgcatcctg gctggagccc gtgctgctgt actcgctcgc tgcgatcagc ggctaccgcc
     1261 tgctgctgcc ggggcagatc gatgaactca accatgtgct gctcgatccg ccggacggcg
     1321 acagcgcgga agccctgcgc tggcgaccgg gctggtgcgc ctggctcgac cagccgaaac
     1381 cccggtcccc ggacgagcgc acgcacgatt ccgcgccccc gcccccgccg cggcgctgcg
     1441 gccggctcgg accggtggcg ctgccgacgg cgctgctgtt cgagcccgac ctgcagcgcg
     1501 gcaacgccgg cgacacgcgc cggcgcctgc tgccgagccg ctatctgcac ctgcgcggcg
     1561 cggcgctcga catggacatc aagcccgaag acgtggccct cgaaaagaag gcagacggcg
     1621 cgcccctcac ctgtgatcgc gatgcccgcc gcgacaacga accgaccgat cccggcgaga
     1681 agcgcgtctg gcagtacgcg cgtgcgctgc gcgtgcagtt tcgcgatctg gccggcgccg
     1741 acctgcgcga tgccagcctg ccgctcggcc agttccagac cgtcaacctg tacggggcga
     1801 tgctcgacgg cgcctatctg aacggcgcgc gcctgtatgg catcgacctg cgctacgcca
     1861 gcctgacctg gagccacctc gacaacagcg actgggacgg cgtgcaggcg gatttcagcg
     1921 ccctcgacca tgccgacctg cgctgcgcgc gcatcctgcg taccgatctg gtcggggcac
     1981 ggatgatcga ggtcgagacc gcccgcctgc acctggagca cagcgacctg cgcggcgccc
     2041 tgatcgtcag tcagccgccc cggccggatt ccgatccgcg gcccgcgccg gagaacaggt
     2101 cagtgctcgc cggcctgacg gccacccggg gatcggctgc cgagctgctg aaggaacggg
     2161 cgctgcgacg ctgcccgccc ggcgcctcct ccgtgcagtc cgtgccggca aagctcccct
     2221 cgcctccgcc cctgttcggg atcgacgaca cccagggtcc ggcggcagcc ggccggcagg
     2281 cggcccagat gacctgcacg ctgcaggaag tgcagcccga agatttccgg gagccgccgc
     2341 cgcggcggct gccgacccgc tatgccaacg cgctggtgct gatggcaccg ccatttgccg
     2401 cgcagggttt cgaacagtcg acggagtggc tcgaagccgt caatcaggtg ttcacgcagc
     2461 agccggtctg cccggcggcc gaggatgccc tgctgcgggc ccggctggcg ttcgccctgc
     2521 agccgaaagc cacgcccgcc gcccccacgg ctgcgcagcg ctgcgagcca cgccccgccg
     2581 actgaagccc cggctggcgg ccggtcgcgg cagcaaccac actgcccgcc accccgatcc
     2641 gcgcaggtcc gctgcggcat gagtgagcgc tattccatca tccgcccgac cccgctcggg
     2701 cgcgccgagc ttgagcgcgt cgccgcgacg ctgccggacc tgatcaccgc caccggcccg
     2761 gatggcgaac tgcgcctgag cgtgcagcgc gccggcggtc tggtgcagcc gatcgactgg
     2821 cagtacggca cgctgtccgt cgacctgccc gatgccgacg tgctcgccgt gctgatcgaa
     2881 ctggcggcgc ggctctgcgc gcgcgtgcgc gacgacagcc tgatgacctg gcgcacgccc
     2941 ttcgacagct acgtgcatgc cgatgacacc gaagccgtcc gcgcccagcg ccagcggctc
     3001 gatgccttgc ggcgccggcg gcgcctgcgc gcctggctgc gtgccggcct gatcgccatg
     3061 accggcatcg tcgtgctgct catcagccac ccggcgttct gggcaagacc gctgaccgat
     3121 ccgggctcgc gcttcgcgct gccgcccgac tggcgcgcgg gcttcaccgg cgggcggccc
     3181 gacgtgctgc tgctgccggc cgacgatttc agcgaggcct atgccgcgca cctcggcgag
     3241 cgcctgcacg agctgaccgg cctgtcggtc aggaccacgc tcgccgtcgg cctcggccgc
     3301 gtgcagctct ttccggacag tccgcagtac gacgcgcaga ccctcgtcga aaccgccacg
     3361 ccggcgatcg accggctgcg cgcgcagtac ggcgccgtgc cggtgatcct gctgacgcag
     3421 cgcgacatca acgcccgcga gcgcagcacc cgcttcagct tcgcgcagca ctaccccggc
     3481 tggcgcgtca gcgtcgtgtc ctcggcgcgc ctgctgccgg gcacgctgat cggccgcgcc
     3541 agcgacgcga cgctggaaac ccggctgctg aagttcctgc tgcgcagcgt cggccagcag
     3601 gtctaccgcc tgccgcgcga caccgacatc gacagcgtca tgtacgcgcc gatcatgagc
     3661 ctgtcggatc tcgaccgcat gggcatgaag ctcgcgcgac cgggagcgcc ctgaacgcag
     3721 cggaaa
//
LOCUS       DAOQIE010000348         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15512_length_3705_cov_4.669589, whole genome shotgun sequence.
ACCESSION   DAOQIE010000348 DAOQIE010000000
VERSION     DAOQIE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15512_length_3705_cov_4.669589"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PK144_16505"
     CDS             <1..1099
                     /locus_tag="PK144_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67374.1"
                     /translation="PLPDALLIGAALGLGLTAAGWLAGATTLARRARFGTLGALLLWA
                     LLAAIAVPALSGYRMEVRFAATGTDLTGRLQHWRDGWSLQPDDLSARLFGTGLGSFPR
                     LYQTLIPAGRQVGSYAFARDGERPLLRLGGGNDLLLGQRIELPGPGVYRVEALARAIG
                     EPALLLARLCWHHVLSCSPGSAFEARAPVQPGAWQHIRFEIDAGRLAQQPAWARWPGT
                     LLIGDQQPDTRIEIAELSLRGPDGHERLANRDFAAGGDRWLSYNEFEHLVWHPKSLPL
                     ALLIETGIAGLTAFVLVLGAAAWGAWRSAVATPAFGTLCGLLGLIGVGAADSALDFPR
                     IAVLFWIGVLMLLATPARAARPWRRAPAAHD"
     gene            1099..2457
                     /locus_tag="PK144_16510"
     CDS             1099..2457
                     /locus_tag="PK144_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNF67375.1"
                     /translation="MRSPRRGRPAGFAAGLLLLVAGLATADVLDAARARFQVSGEPLP
                     GRFPPAGDWRGIGAGPAREPLPAGSNLVPVANTDELRSAMAVALPGDVLLLAPGDYPL
                     QRTLYAERPGEAGRPVRVLAERPGSVRIRVATTVGFELRAPYWWFEGLDIAGVCADHG
                     DCEHAFHIVGDARHTVLRNNRLHEFNAPVKINGSERDGRLQTPDDGRIDGNSLYNSGV
                     RLTGNPVTLIDAVAVNGWTVRANLIADFVKGGGDNISYGAFFKGAGSGNVFERNLVIC
                     EMNLHPPYGARVGLSFGGGGTGAPYCRDGRCAAEHFDGRIRNNLILHCNDVGVYLNRA
                     ARTEVAHNTLYATLGMDARFPDTDAVFTNNLLDGRIKARDGGRWSGDGNLRLEAAGFA
                     ARFFAPQDADFRLRDGSGIVAAGVPLPQVPDDLCGHVRARLPDIGAIEYGLDACDPAG
                     RP"
     gene            complement(2492..>3705)
                     /locus_tag="PK144_16515"
     CDS             complement(2492..>3705)
                     /locus_tag="PK144_16515"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNF67376.1"
                     /translation="ANSPRCSTRSLHRAEPRRPSSAARMALWGVVALLIGYGSLYPFR
                     FRFPGLPLESLLQVPPLWTRPSDVFGNMLLFVPWSLCGWWAGRGTAWRRAAPVLLGGL
                     AWALLLQVLQLWLPERVASLGDVFWNALGLAAGLLLATRLHDAPRTACIAILALWLLG
                     LLLPLLPFWPAGLPLAWVRSLQATLSLPLPAALVWHVAGGWLSMRLLQGLGAGVRAAV
                     GLVVGVLLLGPALPAAHPDPAQMLGLPLGVLCALYGRERADGLLIGLIAAVYTFDGLW
                     PFRWRPLGMLSLQPFDSLLHGNMLNNLRHLCARLAIYAGVLWLIQQRCQRLPPLATLL
                     VVGWVVGVEGAQAWLVGRYADIGELFWVLLAAGLVHLAGPLHPVAGAAPVSGPHGLDP
                     LASTAGRSGEG"
BASE COUNT          527 a         1418 c         1318 g          442 t
ORIGIN      
        1 gccgctgccg gatgcgctgc tgatcggtgc cgccctcggc ctcgggctca ccgccgccgg
       61 ctggctggcc ggcgcgacga cgctcgcgcg gcgcgcgcgt ttcggcacgc tcggggcgct
      121 gctgctgtgg gcgctgctcg cggcgatcgc cgtgccggcg ctgtcgggct atcgcatgga
      181 ggtgcgcttc gccgcgaccg gcaccgacct caccggccgc ctgcagcact ggcgcgacgg
      241 ctggtcgctg cagcccgatg atctgtccgc gcggctgttc ggcaccggcc tcggcagctt
      301 cccgcgcctg taccagacgc tgatcccggc cggtcgccag gtcggcagct acgccttcgc
      361 ccgcgacggc gaacgcccgc tgctgcgcct cggcggcggc aacgacctgc tgctcggcca
      421 gcgcatcgag ctgcccggcc ccggcgtcta ccgcgtcgag gcgctggcgc gcgcgatcgg
      481 cgaaccggcg ctgctgctcg cccgcctctg ctggcaccac gtgctgagct gcagtccggg
      541 ttcggcgttc gaagcccgcg cgccggtgca gccgggcgcc tggcagcaca tccgcttcga
      601 aatcgatgcc ggccggctgg cgcagcagcc cgcctgggca cgctggccgg gcacgctgct
      661 gatcggcgac cagcagccgg acacgcgcat cgagatcgcc gaactgtcgc tgcgcggccc
      721 cgacgggcac gaacggctcg ccaaccgcga cttcgctgcc ggcggcgatc gctggctcag
      781 ctacaacgag ttcgagcatc tggtctggca cccgaagagc ctgccgctgg cgctgctgat
      841 cgagaccggc atcgccggac tcacggcctt cgtgctcgtg ctcggcgcgg ctgcctgggg
      901 cgcctggcgc agcgccgtcg ccaccccggc cttcggcacg ctgtgcggcc tgctcggcct
      961 gatcggggtc ggtgccgccg acagcgcgct cgacttcccg cgcatcgccg tgctgttctg
     1021 gatcggcgtg ctgatgctgc tggccacgcc ggcccgtgcc gcccggccgt ggcggcgagc
     1081 gccggcggca cacgactgat gcgcagcccc cgccgcggcc ggccagccgg cttcgccgcc
     1141 ggcctgctgc tccttgttgc gggcctcgcg accgctgatg tcctcgatgc ggcacgcgcg
     1201 cgcttccagg tgagcggcga gccgctgccg gggcggtttc cgccggccgg cgactggcgc
     1261 ggcatcggcg ccggcccggc gcgcgaaccg ctgccggccg gcagcaatct ggtgccggtc
     1321 gcaaacaccg acgaactgcg cagcgcgatg gccgtggcgc tgcccggaga cgtgctgctg
     1381 ctcgcgcccg gcgattaccc gctgcagcgc acgctgtacg ccgagcgtcc gggcgaggcc
     1441 gggcggccgg tccgcgtgct cgccgagcgc ccgggcagcg tgcgcatccg cgtcgcgacc
     1501 acggtcggct tcgaactgcg cgcgccgtac tggtggttcg agggtctcga catcgccggt
     1561 gtctgcgccg atcacggcga ctgcgaacac gccttccaca tcgtcggcga tgcccgccac
     1621 acggtgctgc gcaacaaccg cctgcacgaa ttcaacgcgc cggtgaagat caacggcagc
     1681 gagcgcgacg gccggctgca gacccccgat gacggtcgca tcgacggcaa cagcctctac
     1741 aactccggcg tgcgcctgac cggcaacccc gtgacgctga tcgacgcggt cgccgtcaac
     1801 ggctggacgg tgcgcgccaa cctgatcgcc gacttcgtca agggcggcgg cgacaacatc
     1861 agctatggcg ccttcttcaa gggtgccggc agcggcaacg tgttcgaacg caatctggtc
     1921 atctgcgaga tgaacctgca cccgccctac ggcgcgcgcg tcggcctgtc cttcggcggc
     1981 ggcggcaccg gggcgccgta ttgccgcgac gggcgctgcg cagccgaaca cttcgacggc
     2041 cgcatccgca acaacctgat cctgcactgc aatgatgtcg gcgtgtacct gaaccgcgcc
     2101 gcgcgcaccg aggtcgcgca caacacgctg tacgcgacgc tcggcatgga cgcgcgcttc
     2161 ccggacaccg atgccgtgtt cacgaacaac ctgctcgacg gccgcatcaa ggcccgcgac
     2221 ggcgggcgct ggagcggcga cggcaacctg aggctcgaag ccgccggctt cgccgcgcgc
     2281 ttcttcgcac cgcaggacgc cgacttccgg ctgcgcgacg gcagcggcat cgttgccgcc
     2341 ggcgtgccgc tgccgcaggt tccggacgat ctctgcggcc acgtccgcgc ccggcttccc
     2401 gacatcggcg ccatcgaata cggcctcgac gcctgcgatc cggccgggcg gccgtaagac
     2461 aggcaatccg gagcgtgcga cggcctgcgg ctcagccctc gccgctgcgg ccggcggtgc
     2521 ttgcgagcgg gtccaggccg tgcggcccgc tcaccggcgc tgcgcccgcc accgggtgca
     2581 gcggtccggc cagatgcacg aggccggcgg cgagcagcac ccagaacagc tcgccgatgt
     2641 cggcatagcg cccgaccagc caggcctgcg cgccctcgac gccgacgacc cagccgacca
     2701 ccagcagcgt ggcgagcggc ggcaggcgct ggcagcgctg ctggatcagc cacagcacgc
     2761 cggcgtagat cgccagccgc gcacagagat gccgcaggtt gttcagcatg ttgccgtgca
     2821 gcaggctgtc gaacggctgc agcgacagca tgccgagcgg ccgccagcgg aacggccaga
     2881 ggccgtcgaa ggtgtagacg gcagcgatca gcccgatcag caggccgtcg gcgcgctcgc
     2941 gaccatacag cgcgcacagc acgccgagcg gcaggccgag catctgcgcc ggatcgggat
     3001 gggcggcggg cagcgccggc ccgagcagca gcacgccgac gaccaggccc acggccgcac
     3061 gcaccccggc gccgagcccc tgcagcagcc gcatcgacag ccagcccccg gcgacgtgcc
     3121 agaccagcgc cgccggcagc ggcaggctca gcgtcgcctg cagcgaacgc acccaggcga
     3181 gcggcaggcc ggccggccag aacggcagca gcggcagcag caggccgagc agccagagcg
     3241 cgaggatcgc gatgcaggcg gtgcgcggcg catcgtgcag gcgcgtggcc agcagcaggc
     3301 cggcggcgag cccgagcgca ttccagaaca cgtcgccgag cgaggcgacg cgctccggca
     3361 gccagagctg cagcacctgc agcagcagcg cccaggccag accgccgagc agcaccggcg
     3421 cggcgcggcg ccaggccgtg ccgcgcccgg cccaccagcc gcacagcgac cacggcacga
     3481 acagcagcat gttgccgaac acgtcgctcg ggcgcgtcca cagcggcggt acctgcagca
     3541 ggctttccag cggcaggccc gggaagcgga agcggaacgg atacagcgag ccgtagccga
     3601 tcagcagcgc gacgacgccc cacagcgcca tgcgcgccgc gctcgacggg cggcgcggct
     3661 cagcgcgatg caacgagcgc gtcgagcagc gcggcgagtt cgccg
//
LOCUS       DAOQIE010000349         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15538_length_3701_cov_6.953374, whole genome shotgun sequence.
ACCESSION   DAOQIE010000349 DAOQIE010000000
VERSION     DAOQIE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15538_length_3701_cov_6.953374"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..815
                     /locus_tag="PK144_16520"
     CDS             138..815
                     /locus_tag="PK144_16520"
                     /inference="COORDINATES: protein motif:HMM:NF025769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HNF67377.1"
                     /translation="MAKKKKHKPDPESWKFKRGRTQRGHIDSCASGYTQTAKNRFRQV
                     HHVVPVSSCSDATISKYVTAAKLKLLHNCMAETDWKIDAAKNVISLPLKPVYLDKRAP
                     AGWDKLPCHQVEHNPAYTDAVSDYLKDNVWNKVQKQAKSCELDPEDLKKKMEDASDHW
                     RDFLTDRGKEHGGTKKCWDKQMSMPDTWYIPFSMNPGTPTPRAPVKWDDLSGSIKEKL
                     KQLFQLH"
     gene            847..1452
                     /locus_tag="PK144_16525"
     CDS             847..1452
                     /locus_tag="PK144_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67378.1"
                     /translation="MSEYKPEFVIWTSRYVEGWCTLLRPEPYEDLYELDYGQPLADRW
                     PADSVSRMDPDRPKDLQLADSVRCPYARTVSTRLRAALEAAGETRLEFLPLAILNHKG
                     RVAARDYWIVNPPYTLDCIDLDASNARWAGRMLDADDPFTACDRLVLRPEVVPAGVQV
                     FRARFKPSLLLMRRALAEALLNEGLSGLAFGEPDAYRGLAA"
     gene            1454..2257
                     /locus_tag="PK144_16530"
     CDS             1454..2257
                     /locus_tag="PK144_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67379.1"
                     /translation="MDPRDARPLIAGAIEEDLTLYAVGVPPGELPGLFGRVSENYQAL
                     GIAILLASGEQARYRECLVRSAWARLHQLRCAGALQPPARERATSFGEGLFDALAAGH
                     WRLAHDIAEACPPGWIPEGEYEDDFCHMRLIGLYTGDSPPEPAAVQALLARHAQVLDG
                     ARTPRLALGQALFDHDADAFAAAFEDFLREELEARMEARERLGEPDADAARVWMRGYV
                     ATEALALLRVAETAGIAPAGPHPLCPPGSLPAGPCPNFPDPFAEIEAAR"
     gene            2257..3324
                     /locus_tag="PK144_16535"
     CDS             2257..3324
                     /locus_tag="PK144_16535"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNF67380.1"
                     /translation="MIPAPLTPEFDDRSGHALAPLAVADAEGRPVLALVVQATFALFP
                     DLAGRAPPLAPLQRPIALAGEHTGEPGRSSLRREPETAWFKPGTDLVLLGHAQAPGGE
                     PVTQLDAGLRVGATQKIVRVFGDRAWTDTGAGLSVSGPQPFQRLPLVWERAFGGVDAA
                     DAQRRCEPRNPVGCGFVAPGSRLAGPQRMPNLEDPAAPLRQPGDCPPPAGFGFIAPHW
                     QPRAALAGTYDAGWQASRAPRLPADFQPAFLNAAAPGLVVPGRLRGDEPVIVLNAAAV
                     PRLAFALPGYPPPPLRIDWRKGGHVRLDAQLDTVVIDTDAMQLLLTWRAHVVLPARGL
                     HAVAAIRARAGRPQALAGARP"
     gene            3321..>3701
                     /locus_tag="PK144_16540"
     CDS             3321..>3701
                     /locus_tag="PK144_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67381.1"
                     /translation="MSRALERFYRGLYVEHVDTAGFLYEQWATRLMESAPAARALVEL
                     ECRLEPHLDALLLGGERALAVALEAAAGGEPGERHIAARLFCRLRHAAGLRATLDGYD
                     ALAADDAAAAAGLRTALARALAWEL"
BASE COUNT          523 a         1386 c         1302 g          490 t
ORIGIN      
        1 agccttcgcg ctgcaccagt tcgcccgccg ctgcgcgccg ccgggcagcg cgctgctggc
       61 gctggcttcc gacaacggcg cgcgcgcggc gctgcgcctc gatccgcccg cgggcgactg
      121 aacgcacgac cgacacgatg gccaagaaga agaaacacaa gccggatccg gaaagctgga
      181 agttcaagcg cggccgcacg cagcgcggcc acatcgacag ctgcgcgagc ggctacacgc
      241 agaccgcgaa gaaccgcttc cggcaggtgc atcacgtcgt gccggtcagc agctgctcgg
      301 acgcgacgat ctcgaagtac gtgacggccg ccaagctcaa gctgctgcac aactgcatgg
      361 ccgagaccga ctggaagatc gacgctgcca agaacgtcat ctcgctgccg ctgaagcccg
      421 tgtatctcga caagcgcgcg ccggccggct gggacaagct gccctgccat caggtcgagc
      481 acaatccggc ctataccgat gctgtcagcg actatctgaa ggacaacgtc tggaacaagg
      541 tccagaagca ggccaagagc tgcgagctcg accccgagga cctgaagaag aagatggagg
      601 acgcgagcga tcactggcgc gacttcctga ccgatcgcgg caaggagcac ggcggcacca
      661 agaagtgctg ggacaaacag atgagcatgc cggacacctg gtacatcccg ttctcgatga
      721 acccgggcac gccgacgccg cgtgcgccgg tgaagtggga tgacctgagc ggcagcatca
      781 aggaaaagct caagcagctg ttccagctgc attgacagcc agcccggcgc ggggaggcag
      841 gcacgcatgt ccgaatacaa gcccgagttc gtgatctgga ccagccgcta cgtcgagggc
      901 tggtgcacgc tgctgcggcc cgagccctac gaggatctct acgagctcga ctacggccag
      961 ccgctcgccg atcgctggcc ggccgattcg gtcagccgca tggacccgga ccggccgaag
     1021 gacctgcagc ttgccgacag cgtgcgctgc ccgtacgcgc gcacggtctc gacgcggctg
     1081 cgcgctgcgc tcgaagccgc cggcgagacg cggctcgaat tcctgccgct cgccatcctc
     1141 aaccacaagg gccgggtcgc ggcgcgcgac tactggatcg tcaatccgcc gtacacgctc
     1201 gactgcatcg acctcgacgc ctcgaacgcc cgctgggccg gcaggatgct cgacgccgac
     1261 gatccgttca cggcctgcga ccggctggtg ctgcgccccg aggtcgtgcc ggccggggtg
     1321 caggtgttcc gcgcgcgctt caagccgagc ctgctgctga tgcgccgtgc gctggccgaa
     1381 gcgctgctga acgagggcct gagcggcctc gccttcggcg aaccggacgc ctaccgcggg
     1441 ctggcggcct gacatggacc cgcgcgatgc acggccgctg atcgccggcg cgatcgagga
     1501 ggacctgacg ctctatgccg tcggcgtgcc gcccggcgag ctgcccgggc tgttcggccg
     1561 ggtatcggag aattatcagg cgctcggcat cgccatcctg ctcgcgagcg gtgaacaggc
     1621 gcgctaccgc gagtgcctgg tgcgcagcgc ctgggcgcgg ctgcaccagt tgcgctgcgc
     1681 gggcgcgctg cagccgccgg cgcgcgagcg tgcgaccagc ttcggcgagg gcctgttcga
     1741 tgcgctcgcc gccggccact ggcggctggc tcacgacatc gccgaagcct gcccgccggg
     1801 ctggatcccc gaaggcgagt acgaggacga cttctgtcac atgcggctga tcggcctgta
     1861 caccggcgac agcccgccgg agccggccgc cgtgcaggcg ctgctcgcgc gtcacgcgca
     1921 ggtgctcgac ggcgcgcgca cgccgcggct cgcactcggg caggcgctgt tcgatcacga
     1981 cgccgatgcc ttcgcggcgg ccttcgagga tttcctgcgc gaggagcttg aagcgcgcat
     2041 ggaagcccgc gagcgcctcg gcgagccgga cgcggacgcg gcgcgggtgt ggatgcgcgg
     2101 ctacgtcgcg accgaggcgc tggcgctgct gcgcgtggcc gaaaccgccg gcatcgctcc
     2161 ggccggtccg catccgctct gtccgcccgg ctcgctgccg gccgggccct gcccgaactt
     2221 cccggacccc ttcgctgaaa tcgaggccgc acgctgatga tcccggcccc gctgacgccc
     2281 gagttcgacg accgcagcgg ccacgcgctg gcaccgctcg ccgtcgccga tgccgagggc
     2341 cggccggtgc tggcgctggt ggtgcaggcg accttcgcgc tgttcccgga cctcgccggt
     2401 cgcgccccgc cgctcgcgcc gctgcagcgc ccgatcgcgc tcgccggcga gcacaccggc
     2461 gagcccgggc gctccagcct gcgccgcgaa cccgagaccg cctggttcaa gcccggcacc
     2521 gatctcgtgc tgctcggcca tgcgcaggcg cccggcggcg agccggtcac gcagctcgat
     2581 gccggcctgc gcgtcggagc cacgcagaag atcgtgcgcg tgttcggcga ccgcgcctgg
     2641 accgacaccg gcgccggcct gtcggtcagc ggtccgcagc ccttccagcg cctgccgctc
     2701 gtctgggagc gcgccttcgg cggcgtcgat gccgccgatg cgcagcgccg ctgcgagccg
     2761 cgcaatcccg tcggctgcgg cttcgtcgca ccgggcagcc ggctcgccgg cccgcagcgc
     2821 atgccgaacc tcgaagaccc ggccgcgccg ctgcgccagc ccggcgactg tccgccgccg
     2881 gccggcttcg gcttcatcgc cccgcactgg cagccgcgcg cggcactcgc cggcacctat
     2941 gacgccgggt ggcaggcgag ccgcgccccg cggctgccgg cggacttcca gccggccttc
     3001 ctgaacgccg ccgcgccggg cctcgtcgtg cccgggcggc tgcgcggcga cgagccggtg
     3061 atcgtgctga acgccgccgc ggtgccgcgg ctcgccttcg cgctgcccgg ctacccgccg
     3121 ccgccgctgc gcatcgactg gcgcaagggc gggcatgtgc gcctcgatgc gcaactcgac
     3181 accgtcgtca tcgataccga cgcgatgcag ctgctgctga cctggcgtgc gcacgtcgtg
     3241 ctgccggcgc gcggcctcca tgccgtcgcg gcgatccgcg cgcgcgccgg ccggccgcag
     3301 gcgctggcgg gagcacgccc atgagccggg cactcgaacg cttctaccgc gggctgtacg
     3361 tcgagcacgt cgacaccgcc ggcttcctgt acgaacagtg ggcgacgcgg ctgatggaat
     3421 cggcgccggc cgcgcgcgcg ctggtcgagc tcgaatgccg gctggagccg cacctcgatg
     3481 cgctgctgct cggcggcgag cgcgcgctcg ccgtcgcact cgaagccgct gccggcggcg
     3541 agcccggcga gcgtcacatc gccgcgcggc tgttctgccg gctgcgccat gccgccggcc
     3601 tgcgcgccac gctcgatggc tatgacgcgc tcgccgccga cgatgcggcg gctgccgcgg
     3661 gcttgcgcac cgcgctggcg cgggcgctgg cctgggagct g
//
LOCUS       DAOQIE010000350         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15555_length_3699_cov_6.679473, whole genome shotgun sequence.
ACCESSION   DAOQIE010000350 DAOQIE010000000
VERSION     DAOQIE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15555_length_3699_cov_6.679473"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..502
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19..54
                     /rpt_unit_seq="gttttccgcggtgtaatgccgcggcctcattgaagc"
     gene            744..1703
                     /locus_tag="PK144_16545"
     CDS             744..1703
                     /locus_tag="PK144_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HNF67382.1"
                     /translation="MSHWNKVYELHRILSERTQPVSQATLLQELDCAPATLKRVLQTL
                     RDLSAPVDNVRGLGWRYGEDVRGRFELPGLWLSNEELHALALLDQLLDQIDPGLLRRP
                     LAPVRARIRQMKALLPDAQALGRLKLLPQGRREVRPATLGTIARATVARRRLRIDYHS
                     RSKDEHTAREVSPQHLARYRDNWYLDAWCHQRGALRRFAIDRVHGAEQLGEVAHDVPP
                     ETLQAHLGSAYGIFAGPAAATAILHFSPDRARWIADEHWHDDQHGHWLDDGRYELHVP
                     YSDPTELLMDVLRHVPDVEVIAPQDLRSAFVQRLRAGLERHAR"
     gene            1844..2050
                     /locus_tag="PK144_16550"
     CDS             1844..2050
                     /locus_tag="PK144_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HNF67383.1"
                     /translation="MNEVIFLVEETVEGGYQARALGHSIHTQADSLPELREQVRDAVN
                     CHFDADALPALIRLHFVREEVLTP"
     gene            2174..>3699
                     /locus_tag="PK144_16555"
     CDS             2174..>3699
                     /locus_tag="PK144_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF67384.1"
                     /translation="MDFPTFFHTTTGHTPYPYQQRLATGPWPELLDVPTGLGKTAAVV
                     VAWLWRRVVNGEALPRRLVYCLPMRTLVEQTEGCCRNWLDKAGLAAQVDLHVLMGGRT
                     GSDWVEQPERPAILIGTQDQLLSRALMRGYAMSRYRWPVDFALLHNDALWVYDEVQLM
                     GPGLPTGAQLEAFRRAPDWPTARPGRSLWLSATLHPDWLATVDFAAAVPGLVRHGIEA
                     ADRADAAVTRRLLARKRLQPAGIRLTAADARSGAYARSLAAQALATHAADSLTLLVLN
                     TVERAQQVWKALNAQRPAAECVLLHGRFRPGDRQRQQARLDAPLPAAGRIVVATQCLE
                     AGVDVSARTLFTELAPWASLVQRCGRCNREGEYNDTAEAAVYWIDCDDPALAAPYPAA
                     DLDAARAVLAELGDAGPDALPKVAADAALHPVLRRRDFLELFDTDPDLSGFDVDVSAW
                     IRDADDADLLLFWRELPVAKNGFDADGQPRPAAAELCRAGIGAASAFLKSRREAAFVW
                     "
BASE COUNT          560 a         1332 c         1253 g          554 t
ORIGIN      
        1 gggcatgctg cgcctgcggt tttccgcggt gtaatgccgc ggcctcattg aagccacctg
       61 ttgcccgccg gccgccgtat cggccgccag cgttttccgc ggtgtaatgc cgcggcctca
      121 ttgaagctag atcgagtccg ggcggacaaa gagggcggca acgatgggtt ttccgcggtg
      181 taatgccgcg gcctcattga agcgaggctc tggaaggcgc cggcctgacc gccgggagga
      241 ttcgttttcc gcggtgtaat gccgcggcct cattgaagcc gggcaagaca cggtctccag
      301 catggtcggg acgcccatgt tttccgcggt gtaatgccgc ggcctcattg aagccacgac
      361 ccgagcgagc gcccgcagcg cggcgacgcg ctggttttcc gcggtgcaat gccgcggcct
      421 cattgaaaca tgcagatggt cggcaagaaa tccgccggcc gcctgcgttt tccgcggtgt
      481 aatgccgcgg cctcattgaa gctgaaattc agccggctgc aggagatgca atccaatgaa
      541 ggattccgcg acttgcaacc gtgatgcgcc ggcaacgatt gcaccaccag caggcaggac
      601 atgggcgcat ccgcgcctct gcccgccatg gggtgatgca aggcgattga tggccgtcat
      661 ggctgccgga cgcacgcagt cgcataaccg cacccgtgcg catcgccgat gcgtgacgac
      721 gctgcagggc aaggagcccg tgcatgagcc actggaacaa ggtttatgag ctgcaccgca
      781 tcctgtcgga gcgcacgcag ccggtatcgc aggccacgct gctgcaggaa ctcgactgcg
      841 cacccgccac gctcaaacgt gtcctccaga ccctgcgcga cctgagcgcg ccggtcgaca
      901 acgtgcgtgg cctgggctgg cgttacggtg aggacgtgcg cgggcgcttc gagctgcccg
      961 ggctctggct ctccaatgaa gaactgcacg cgctggcgct gctcgatcag ttgcttgacc
     1021 agatcgaccc cggcctgctg cgccggccgc tggcgccggt gcgcgcccgc atccggcaga
     1081 tgaaggcgct gctgcctgac gcgcaggcgc tcggacgcct gaagctgctg ccgcagggcc
     1141 ggcgcgaagt gcgcccggca acgctcggca cgatcgcccg cgccaccgtc gcacgccggc
     1201 gcctgcgcat cgactaccac tcgcgcagca aggacgagca caccgcccgc gaagtctcgc
     1261 cgcagcacct cgcccgctac cgcgacaact ggtatctcga cgcctggtgc caccagcgcg
     1321 gcgccctgcg ccgcttcgcc atcgatcgcg tgcatggcgc cgagcaactc ggcgaagtcg
     1381 cgcacgacgt gccgcccgaa accctgcagg ctcacctcgg cagcgcctac ggcatcttcg
     1441 ccggccccgc cgccgccacc gccatcctgc acttcagccc cgaccgcgcc cgctggatcg
     1501 ccgacgaaca ctggcacgac gaccagcacg gccactggct ggacgacggg cgctacgagt
     1561 tgcacgtgcc ctacagcgac ccgaccgaac tgctgatgga cgtgctgcgg catgtgccgg
     1621 acgtggaggt gatcgcaccg caggacttgc gctcggcctt cgtgcagcgg ctgcgggcgg
     1681 ggctggaaag gcatgcgcgc tgagagaagg ccggagggcc gggtgcgcag cgaccgatcg
     1741 tggagaccgg caccttcggc ccgccgcatg gctccctgcc tgccggcctt gcgccttgcg
     1801 acatacgctg ttgtcagccg gaaaattcag acgggccacg acgatgaacg aagtcatctt
     1861 tctggtcgag gaaacggtcg agggcggcta tcaggcccgg gcgctcgggc attcgatcca
     1921 tacccaggcc gactcgctgc ccgaactgcg cgaacaggtg cgcgatgccg tcaactgcca
     1981 cttcgatgcc gacgcgctgc cggcgctgat ccggctgcac ttcgtgcgcg aggaagtgct
     2041 gacgccgtga agctgccgcg cgagccgagc ggacagatca tcacgcacac gtccggtact
     2101 cacccagggc tcagtaattg agcccacacc cagtaacagt ggccggatcg tgaccggccc
     2161 cggaacggac gccatggact tccccacctt cttccacacc accaccggcc acacccccta
     2221 cccctaccag cagcggcttg ccaccggccc gtggccggag ttgctcgatg tgccgaccgg
     2281 cctcggcaag accgcggcgg tcgtggtcgc gtggctgtgg cggcgggtcg tgaacggcga
     2341 ggcgctgccg cggcggctgg tctactgcct gccgatgcgc acgctggtcg agcagaccga
     2401 aggctgctgc cgcaactggc tggacaaggc cgggctggcc gctcaggtcg atctgcatgt
     2461 gctgatgggc ggccggacgg gttcggactg ggtcgagcag cccgagcggc cggcgatcct
     2521 gatcggcacg caggatcagt tgctgtcgcg cgcactgatg cgcggctacg cgatgagccg
     2581 ctaccgctgg ccggtcgatt tcgcgctgct gcacaacgat gcgctgtggg tctacgacga
     2641 agtgcagctg atgggtccgg gcctgccgac cggcgcgcag ctcgaagcct tccgccgcgc
     2701 ccccgactgg ccgaccgccc gcccgggccg cagcctgtgg ctgtcggcga cgctgcatcc
     2761 ggactggctc gcgaccgtcg atttcgccgc tgccgtgccc ggtctggtcc ggcacggcat
     2821 cgaggctgcg gaccgcgccg atgcagccgt cacccggcgg ctgctggccc gcaagcgcct
     2881 gcagccggcc ggcatccggc tgacggccgc cgacgcccgc tccggcgcct atgcgcgcag
     2941 cctcgccgcg caggcgctcg ccacgcacgc cgccgactcg ctgacgctgc tggtgctgaa
     3001 caccgtcgag cgcgcccagc aggtctggaa ggcgctgaac gcgcagcggc cggccgccga
     3061 gtgcgtgctg ctgcacggcc gcttccggcc cggcgaccgc cagcgccagc aggcgcggct
     3121 cgatgcgccg ctgccggcgg ccgggcgcat cgtggtcgcg acgcagtgcc tggaagcggg
     3181 agtcgatgtc tcggcgcgca cgctgttcac cgaactcgcg ccgtgggcct cactggtgca
     3241 gcgctgcggg cgctgcaacc gcgagggtga gtacaacgac acggccgagg ccgccgtcta
     3301 ctggatcgac tgcgacgacc cggcgctcgc cgcgccgtac ccggccgccg acctcgatgc
     3361 cgcccgcgcg gtgctggccg aactcggcga tgccggcccc gatgccctgc cgaaagtcgc
     3421 cgccgacgcg gcgctgcatc cggtgctgcg ccggcgcgac ttcctcgaac tgttcgatac
     3481 cgatccggac ctgagcggct tcgatgtcga tgtctcggcc tggatccgcg acgccgacga
     3541 tgccgacctg ctgctgttct ggcgcgagtt gccggtcgcg aagaacggtt tcgacgccga
     3601 cggccagccg cgccccgcag ccgccgaact ctgccgcgcc ggcatcggcg ccgcgagcgc
     3661 cttcctgaag tcgcgccgcg aggccgcttt cgtctggga
//
LOCUS       DAOQIE010000351         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15594_length_3694_cov_6.892553, whole genome shotgun sequence.
ACCESSION   DAOQIE010000351 DAOQIE010000000
VERSION     DAOQIE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15594_length_3694_cov_6.892553"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PK144_16560"
     CDS             complement(<1..1040)
                     /locus_tag="PK144_16560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF67385.1"
                     /translation="MNAMNRLRPALAVIFDAHGRWGRAALGLFALASLWLTAPANLGL
                     GLALLAAGGALFAGWRRGGRPDAVGFATLALLVWLVLRYGLQRAGIAEPELVKPQSGW
                     LDWMTPLVFAALAVLIPPGCRLPWLRRLWVLALLGCTLGVIGFLIGRGPLVLWSGERL
                     GFHLNRPLGIGLYAGVFVVGLVAARQQWWSLAPPWRWPLRIGGLMLLALHLQVLASAQ
                     NRTTWLALLVVAAGAGLAGLWRRRKGSARPAPRRTGLAVLGAVLLLAVFGYANRQAFE
                     QRLEAEQEVVATVGEAGLAAASASSITTRLRLWQFVLERWPQAPLLGHGFGGLQTVVD
                     TQLRPRAALMAG"
     gene            complement(1037..2134)
                     /locus_tag="PK144_16565"
     CDS             complement(1037..2134)
                     /locus_tag="PK144_16565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF67386.1"
                     /translation="MSPRFVYLAWGRPQRPRANLIQTLHTVEALAALGAGVRLYLPPV
                     PAGFDTGGFLAGMGIRRPIDLRPVESLHRRWGGWPFALLHLGELRRAGVVYTRVPELS
                     RVLARLGIRHWLEVHETDTLDARGQLPALRTALARGTLRGLVAISEAGRAALLRAGIE
                     AARVHVLPSGVDLTAFAAVPALTEADLRAPRALYVGRISRDRGLPLLEHLAAAGVPLT
                     LVGPRDHEPAATHAQLELRPAVAHAAVPAELARGALALMPYQADLRHAASISPIKLFE
                     AMAAGRLVIASDLPPLREIVRDGVNGLLVPPEDPDAWLGAIGRVRKDPAAALAMAEAG
                     RQTASAYGWDARARRLLALSAGAPASAAEAA"
     gene            2290..3420
                     /locus_tag="PK144_16570"
     CDS             2290..3420
                     /locus_tag="PK144_16570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67387.1"
                     /translation="MSAPTTASRPRRLAMVLADLARGGIGKMRVHLANAFAAEGVAVD
                     LLLARTGSPYRELIGPGVRLVDLGTSHAWFGVPRTAAYLLRERPDVVLTQRSRVNALV
                     LRSRALCGLKMPVHSTFNTNQSAQLASLTPAKARSQLAQMRRWYPRNDGLIAISHGVA
                     DDAATLLGLDRAAMRIIYNPVVTPELHARAAGPAGHPWFGDGGAPVILGVGRLEPQKD
                     FATLLDAFARLIAGGADYRLLILGEGKLRGALEAQVAQLGLGGRVALPGFAVNPYPYM
                     AGARLLVMSSAWEGLGNVLVEALALGTPVVSTDCPNGPAEILEHGRHGRLVPVGDAAA
                     LADAIRATLAAPLPAAQLRAATARFTLATAAAQYLDALGLAR"
     gene            3417..>3694
                     /locus_tag="PK144_16575"
     CDS             3417..>3694
                     /locus_tag="PK144_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67388.1"
                     /translation="MTTATGRIALFIPSFSDGGVEKQMVALAGGFAQAGYPTDFLTRP
                     GELPYLDRLDPAVQLIRLPAARHAQREATIDYLRTQRPLVLMSAKGKD"
BASE COUNT          504 a         1438 c         1339 g          413 t
ORIGIN      
        1 tcgccggcca tcagcgccgc ccgtggccgc agttgtgtat cgacgaccgt ctgcaggccg
       61 ccgaagccgt ggccgagcag cggcgcctgc ggccagcgtt cgagcacgaa ctgccagagc
      121 cgcaggcggg tggtgatcga tgaggccgac gccgccgcca gtcccgcctc gccgacggtg
      181 gcgacgacct cctgttcggc ttcgaggcgc tgttcgaagg cctggcggtt ggcgtaaccg
      241 aacacggcca gcagcagcac ggcgccgagc accgccagcc cggtgcgacg cggcgccggg
      301 cgggcgctgc ccttgcgccg gcgccacagc ccggccagcc ccgcccccgc ggccaccacc
      361 agcaaggcca gccaggtcgt gcggttctgg gcgctggcca gcacctgcag atgcagggcc
      421 agcagcatca ggccgccgat gcgcagcggc cagcgccacg gcggcgcgag cgaccaccat
      481 tgctgccggg ccgccacgag cccgacgacg aaaacgccgg cgtacaggcc gatgccgagc
      541 ggccggttca ggtggaaacc cagacgctcg ccggaccaca gcaccagcgg cccgcgcccg
      601 atcaggaaac cgatcacccc gagcgtgcag ccgagcagcg cgagcaccca cagccggcgc
      661 agccacggca ggcggcatcc gggcgggatc aggacagcca gcgcggcgaa caccagcggc
      721 gtcatccagt ccagccagcc ggactgcggc ttcaccagct ccggctcggc gatgccggcc
      781 cgttgcagcc cgtagcgcag gacgagccag acgaggagcg cgagcgtggc gaagccgacg
      841 gcatcgggcc gcccgccgcg ccgccagccg gcgaacagcg caccgccggc ggccagcagc
      901 gccagcccga ggccgaggtt ggccggagcg gtcagccaca ggctcgcgag cgcgaacagc
      961 ccgagcgccg cccgccccca gcggccatgc gcatcgaaga tcacggccag cgccggacgc
     1021 agccggttca tggcgttcat gcagcctccg cagccgatgc cggggcgccg gcactcagcg
     1081 cgagcagccg gcgcgcgcgc gcatcccagc cgtaggcgct tgccgtctgc cggccggctt
     1141 cggccatcgc cagcgccgcg gcggggtcct tgcgcacgcg cccgatcgcg ccgagccagg
     1201 cgtccgggtc ctcgggcggc accagcaggc cgttgacgcc atcgcgcacg atctcgcgca
     1261 gcggcggcag gtcggaggcg atcaccagcc ggccggcggc catcgcctcg aacagcttga
     1321 tcgggctgat cgacgcggcg tggcgcagat cggcctgata cggcatcagc gccagcgcac
     1381 cgcgcgcgag ttccgccggc accgcggcgt gcgcgacggc cgggcgcagt tcgagctgcg
     1441 catgcgtggc ggcgggttcg tggtcgcgcg gcccgaccag cgtcagcggc acgccggccg
     1501 cggccaggtg ctccagcagc ggcaggccgc ggtcgcggct gatgcgcccg acgtacagcg
     1561 cccgcggcgc gcgcagatcg gcttcggtga gcgccggcac cgccgcgaac gccgtcagat
     1621 cgacgccgct cggcagcacg tgcacccgcg ccgcctcgat cccggcccgc agcagcgccg
     1681 cgcggccggc ctcgctgatc gcgaccagcc cgcgcagcgt gccgcgcgca agtgccgtgc
     1741 gcagcgccgg caactggccg cgcgcatcca gggtgtcggt ctcgtggact tcgagccagt
     1801 gccggatgcc gaggcgcgcg agcacccgcg acagctccgg cacgcgcgtg tagacgaccc
     1861 cggcgcggcg cagctcgccg aggtgcagca gcgcgaacgg ccagccgccc cagcgccggt
     1921 gcaggctctc gaccgggcgc aggtcgatcg gccggcggat gcccatgccg gcgagaaagc
     1981 cgccggtgtc gaagcccgcc gggaccggcg gcaggtacag gcgcacgccg gccccgagcg
     2041 cggcgagcgc ctcgacggtg tgcagggtct ggatcaggtt ggcacgcggg cgctgcgggc
     2101 ggccccaggc gaggtagacg aagcgcggcg acatggtgcg gcggccgggg gacggttgcg
     2161 ggagcgcgca ttctatacat gcggcccggc gtgacacgcg ccccggcgcg cccccgcggc
     2221 cacccggccc gccgggacga tccggtatac tcgcggccct ttttcgcccg gccggtgcct
     2281 gccttcccga tgtccgcccc gacgaccgct tcccgccccc ggcggctcgc gatggtgctc
     2341 gccgatctcg cccgtggcgg catcggcaag atgcgcgtgc acctcgccaa cgccttcgcc
     2401 gccgaaggcg tggccgtcga tctgctgctc gcgcgcaccg gcagccccta ccgcgagctg
     2461 atcggccccg gcgtgcgcct cgtcgacctc ggcacctcgc acgcctggtt cggcgtgccg
     2521 cgcacggcgg cctacctgct gcgcgagcgg cccgacgtgg tgctgacgca gcgctcgcgc
     2581 gtcaacgcgc tggtgctgcg cagccgtgcg ctgtgcggcc tgaagatgcc ggtgcacagc
     2641 accttcaaca ccaaccagag cgcgcagctg gcgagcctca caccggccaa ggcacgcagc
     2701 cagctcgcgc agatgcgccg ctggtacccg cgaaacgacg gcctgatcgc gatctcgcac
     2761 ggcgtcgccg atgacgccgc gacgctgctc ggcctcgatc gcgcggcgat gcgcatcatc
     2821 tacaacccgg tcgtgacgcc ggagctgcac gcgcgcgccg ccggaccggc cggacatccg
     2881 tggttcggcg atggcggcgc gccggtgatc ctcggcgtcg gccggctgga gccgcagaag
     2941 gacttcgcga cgctgctcga tgccttcgcg cgcctcatcg ccggcggggc cgattaccgg
     3001 ctgctgatcc tcggcgaagg caagctgcgc ggcgcgctcg aagcgcaggt cgcgcagctc
     3061 ggcctgggcg ggcgcgtcgc gctgccgggc tttgcggtca acccgtatcc gtacatggcc
     3121 ggcgcgcggc tgctggtgat gtcctcggcc tgggaagggc tcggcaacgt gctggtcgag
     3181 gcgctcgcgc tcggcacgcc ggtggtctcg accgactgcc cgaacggtcc ggccgagatc
     3241 ctcgaacacg gccgccacgg ccggctggtg ccggtcggcg atgccgccgc cctcgccgat
     3301 gccatccgcg ccacgctcgc cgcgcccctg ccggccgcgc agctgcgcgc cgcgaccgcg
     3361 cgcttcacgc tcgcgaccgc cgccgcgcag tatctcgacg cgctggggct cgcccgatga
     3421 cgacggcgac cggccgcatc gcgctgttca tcccgtcgtt cagcgacggc ggcgtcgaga
     3481 agcagatggt cgcgctcgcc ggcggcttcg cgcaggccgg ctacccgacc gacttcctga
     3541 cccgccccgg cgagctgccg tatctcgacc ggctcgaccc ggccgtgcag ctgatccggc
     3601 tgccggcggc gagacacgcg cagcgcgagg ccaccatcga ctacctgcgc acgcagcgcc
     3661 cgctggtgct gatgtcggcc aagggcaagg acga
//
LOCUS       DAOQIE010000352         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15684_length_3683_cov_5.711963, whole genome shotgun sequence.
ACCESSION   DAOQIE010000352 DAOQIE010000000
VERSION     DAOQIE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15684_length_3683_cov_5.711963"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PK144_16580"
     CDS             complement(<1..786)
                     /locus_tag="PK144_16580"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNF67389.1"
                     /translation="MRSSIRRLAVLCLSLIAGAALAAPQVRDARVAEARDRVQLILDL
                     SEPARYTTYFVDQPPQVVIDLPDAEPSDALKDRSYFGKSKAIRSIRGGKRQDGGIRLI
                     VRLDQPARPSTWLIPAADGKPDRLVIEMPAGKAQARGERLPDIDFESSPRTAGRPAER
                     APQRLARAVRRDIVIAIDPGHGGKDTGAIGLRDVREKDVVLAIGRRLATLIDREPGMR
                     AVMTRRGDTFLPLRSRMSVARKQKADLFISIHADSAPNGSLASG"
     gene            complement(1019..1510)
                     /gene="tsaE"
                     /locus_tag="PK144_16585"
     CDS             complement(1019..1510)
                     /gene="tsaE"
                     /locus_tag="PK144_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004906013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNF67390.1"
                     /translation="MSAAAPRALPLADPAATARLGAALAAALPAHGGLIVHLYGELGA
                     GKTTLVRSLLHALGHHGAVRSPTYALVEPYTLARGPAYHFDLYRLADPEELEYIGIRD
                     FLDADALLLIEWPQRGGGFVPAADLVVQLDHAGTQRHATLTAASATGQALLTALELPA
                     AQV"
     gene            complement(1507..3021)
                     /locus_tag="PK144_16590"
     CDS             complement(1507..3021)
                     /locus_tag="PK144_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNF67391.1"
                     /translation="MKLPDDRLPERLYSAASVREMDRASIQDHNLPGYLLMTRAAEAC
                     VQALYQHWPQPARAAIICGGGNNGGDGYVLARLLIERGVAVEALWLLPPERLKGDAAM
                     ALRDAVAADVPVRPFSTERLARCDLIVDALLGTGLERPVEGEFLSAIRAINAARAAGA
                     GVLAIDLPSGLAADTGCVLGEAAVIADATVSFIGLKAGLVTGAGPAHAGTLHFADLGA
                     PAAVIAAAPVKALRYSAADRGALLGPRARTAHKGDFGHVLVIGGDAGMGGAVKLCSEA
                     ALRSGAGLVSCATHPGHASHLLSGRPEIMFRSVGSAEALASMLGRASVLAVGPGLGRS
                     EWGRGLWKAALATALPLVLDADGLNLLADAPSRRDHWVLTPHPAEAARLLGCSTAEVQ
                     SNRFASAEALAERFGGVAVLKGAGSVIASARPEDGVPVVIAAGNPGMASGGMGDVLTG
                     IIAALLAQGLTAFDAARLGAWLHAKAADLAVQAGGERGLIAGDLFIPLRRLVNP"
     gene            3177..>3683
                     /locus_tag="PK144_16595"
     CDS             3177..>3683
                     /locus_tag="PK144_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HNF67392.1"
                     /translation="MRTWARELGFTATGISGVDLGADEQRLLDWLAAGFHGDMDYMAR
                     HGVLRARPAELVPGTLRVLSARIDYWPGAGAADAGTVLADGTRAYVARYALGRDYHRI
                     VRPRLARLAQRIEAVTGPFVHRAFADSAPILEKPLAARAGLGWVGKHSNLLARDGSWF
                     LLGELCMAL"
BASE COUNT          512 a         1306 c         1362 g          503 t
ORIGIN      
        1 gccgctggcg agcgagccgt tcggggccga gtcggcgtgg atcgagatga agagatcagc
       61 cttctgcttg cgtgcgacgc tcatgcgcga gcgcaagggc aggaaggtgt cgccgcggcg
      121 ggtcatcacc gcgcgcatgc ccggttcgcg gtcgatcagc gtcgccagcc gccggccgat
      181 cgcgagcacg acgtccttct cgcgcacgtc gcgcaggccg atcgcgccgg tgtccttgcc
      241 gccgtggccg gggtcgatgg cgatgacgat gtcgcggcgc acggcgcgtg ccagccgctg
      301 cggtgcccgc tcggccggcc ggccggcggt gcgcggcgag ctttcgaagt cgatgtcggg
      361 cagccgctcg ccacgtgcct gcgccttgcc ggccggcatc tcgatcacca gccggtccgg
      421 cttgccgtcg gccgccggga tcagccaggt cgacggtcgc gccggctggt cgagccgcac
      481 gatcagccgg atgccgccgt cctggcgctt gccaccgcgg atgctgcgga tggccttcga
      541 tttgccgaag tagctgcgat ccttcagcgc atcggaaggc tcggcgtcgg gcaggtcgat
      601 gacgacctgc ggcggctgat cgacgaaata cgtcgtgtag cgcgccggtt ccgacagatc
      661 gaggatcagc tgcacgcgat cgcgcgcttc ggcgacgcgc gcatcccgca cctgcggcgc
      721 tgcaagggcg gcaccggcga tgagagacag gcagaggacg gccagacgac ggatggacga
      781 gcgcatcggc gtgggcgtgt acgggcggca gaaaagatac gcaggagtat gacacgcacg
      841 acacgtatct gcagggcaag ctgtggcttt tctgcattcg gttgttgcga cagtgatagt
      901 cgttgcgttt cgtgcccgtc acgcaggaat acacgcgctt cagagcttca ttcttcgtgc
      961 aggaaggtcc gggacgacgg ggaaacccga catgcacgcg cgatgcgtgc gaccggcatc
     1021 agacctgcgc ggcgggcagc tccagcgccg tcagcagcgc ctgcccggtc gcgcttgcag
     1081 ccgtgagcgt cgcgtgccgc tgcgtgccgg cgtgatcgag ctgcacgacg agatcggccg
     1141 ccggcacgaa gccgccgccg cgctgcggcc attcgatcag cagcagcgca tcggcatcga
     1201 ggaagtcgcg gatgccgatg tattcgagtt cctcgggatc ggcgagccgg tacagatcga
     1261 agtgatacgc cggtccgcgg gccagcgtgt acggctcgac cagcgcgtag gtggggctgc
     1321 gcaccgcgcc gtgatgaccg agcgcgtgca ggaggctgcg caccagcgtc gtcttgccgg
     1381 ccccgagttc gccgtacaga tggacgatca gaccgccgtg cgccggcagc gccgcggcca
     1441 gcgccgcacc gaggcgggcg gtcgcagcgg ggtcggccag cggcagcgcg cggggggcgg
     1501 ccgcactcac ggattcacga gccggcgcag cgggatgaac aggtcgccgg cgatcaggcc
     1561 gcgctcgccg ccggcctgca cggccagatc ggcggccttg gcgtgcagcc aggcgccgag
     1621 gcgcgccgcg tcgaaggccg tgagcccctg cgcaagcagc gcggcgatga tgccggtcag
     1681 cacgtcgccc atgccgccgc tcgccatgcc ggggttgccg gcagcgatca cgaccggcac
     1741 gccgtcctcg gggcgggccg aggcgatgac gctgccggcg cccttgagca ccgcgacgcc
     1801 gccgaagcgc tcggcgagtg cttcggccga ggcgaagcgg ttggactgga cttcggcggt
     1861 gctgcagccg agcaggcgcg cggcttcggc cggatgcggg gtcagcaccc agtgatcgcg
     1921 ccgggacggg gcatcggcga gcaggttcag gccgtcggca tcgagcacca gcggcagcgc
     1981 ggtggcgagc gccgccttcc agaggccacg cccccattcg ctgcgtccga gtccggggcc
     2041 gacggcgagc acgctggcgc gcccgagcat cgacgcgagc gcctcggcac tgcccacgct
     2101 ccggaacatg atctccggcc ggcccgacag cagatggctg gcgtgtcccg gatgggtcgc
     2161 gcagctgacc agcccggcgc cgctgcgcag ggccgcctcc gagcagagct tcacggctcc
     2221 gcccatgccg gcatcgccgc cgatgacgag cacgtgaccg aagtcgccct tgtgcgccgt
     2281 gcgtgcgcgc ggtccgagca gcgcgccgcg atcggcagcg ctgtagcgca gcgccttgac
     2341 cggtgccgcg gcgatcacgg ccgccggggc gccgagatcg gcgaagtgca gcgtgccggc
     2401 gtgcgccgga ccggcaccgg tgacgaggcc ggccttcagg ccgatgaagc tgacggttgc
     2461 atcggcaatc acggccgcct cgccgagcac gcagccggta tcggcggcga gtcccgaggg
     2521 caggtcgatc gccagcacgc cggcaccggc ggcgcgtgcg gcgttgatgg cgcggatcgc
     2581 gctcaggaat tcgccctcga ccggccgctc caggccggtg ccgagcagcg catcgacgat
     2641 caggtcgcag cgcgccagcc gctcggtgct gaacggccgc accggcacgt cggccgcgac
     2701 cgcatcgcgc agcgccatcg ccgcatcgcc cttgagccgc tccggcggca gcagccacag
     2761 cgcctcgacc gcgacgcccc gctcgatgag caggcgcgcg agcacgtagc cgtcgccgcc
     2821 gttgttgccg ccgccgcaga tgatcgcggc gcgcgcaggc tgcggccagt gctggtacag
     2881 cgcctgcacg caggcctcgg cggcccgcgt catcagcagg taaccgggga ggttgtggtc
     2941 ctggatgctg gcacggtcca tctcgcggac cgaggcggcg ctgtagagac gttcgggcag
     3001 gcggtcgtcg ggtaacttca tggcctcatg ccgcattgca gtatttcggg ttcgggcaca
     3061 atagccttcc gccccgtgtg ccgccagccg caggcccgtc cgtgaccgag ccttcccccc
     3121 cgcccgtacc cgcagcgccg cagacgccgg ccggctgggc ggcgctcgcg gcagacatcc
     3181 gcacctgggc gcgcgagctc ggcttcacgg cgaccggcat cagcggcgtc gatctcggcg
     3241 cggacgagca gcggctgctg gactggctgg ccgccggttt tcacggcgac atggactaca
     3301 tggcccgtca cggcgtgctg cgggcgcggc cggccgaact cgtgcccggc accttgcgcg
     3361 tgctgtcggc gcgcatcgac tactggcccg gggccggggc ggccgatgcc ggaaccgtgc
     3421 tcgccgacgg cacgcgcgcc tacgtcgccc gctacgcact cgggcgcgac taccaccgca
     3481 tcgtgcgtcc gcggctggcg cggctcgcgc agcgcatcga ggccgtgacc ggaccgttcg
     3541 tgcatcgggc tttcgcggat tcggcgccga tcctcgaaaa gccgctggcc gcgcgcgccg
     3601 gcctcggctg ggtcggcaag cattccaacc tgctcgcccg cgacggctcg tggttcctgc
     3661 tcggcgaact ctgcatggca ctg
//
LOCUS       DAOQIE010000353         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_15821_length_3662_cov_8.238703, whole genome shotgun sequence.
ACCESSION   DAOQIE010000353 DAOQIE010000000
VERSION     DAOQIE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_3662_cov_8.238703"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..2681
                     /locus_tag="PK144_16600"
     CDS             474..2681
                     /locus_tag="PK144_16600"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNF67393.1"
                     /translation="MNVAAPSTVPRRRTGFGKYRTIVLSIALFLVFDLGVLILNFYTS
                     SEISTDAVGINLAGRQRMLSQRLVKALHEFGAAPAGSTEATNALMELRNTTTLFDTTL
                     SAFERGGETTGGDGRAVLLQPVSDARGRAAVEAALKLWQPLKTALDPALRGSDAAALH
                     EAVRLASSSNLALLRLMNDLTTALEQVAAGKADRLRLIQTIGIGLALANFFLILFHFI
                     RQLRDSDTRAETARRETVEILDTVTDGLFLLDRELTIGAQPSKSLHGLFGRSTLANQP
                     FLPMLREIVSEKVYREAADFLDVLFREHVNLKLVASLNPLHEVEVLIPGPNGEPQRKY
                     LGIEFTRVTDAHSALVHLLVTVRDITVRIELARQLELSRRQAEEQMSLLLGILHVEPP
                     RLREFIDRADKALHDINATLRRHGSKHLSKRDEYRGVLNEIFPTIHALKGEAGLLGLE
                     FFEKLAHDFEERIVELRGLKRVGGDELIGLAVQLDRLIGSVEAVRAMVERLGSLQQGF
                     AQEATAAAGAPAGAAAETPAMAPTSTAAPLPELTVDTFRFLVERLAQTEHKRVQLLAS
                     GLDTLAVSGAEQITALREVLVQLLRNAVAHGIETPEERHLAGKTDVGTIRLQARADAS
                     AGIELICHDDGRGLDPGRIRAAALQSGRWSEDELDAMGTAGLRALLFEPGFTTAAEAT
                     LAAGRGVGMDVIKHRVEALGGTLSVASEPGRYCAWRITLPGLPGLGELSFETT"
     gene            2727..3107
                     /locus_tag="PK144_16605"
     CDS             2727..3107
                     /locus_tag="PK144_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF67394.1"
                     /translation="MPEPKPLTLLIVDDSHIIRRRIERSVAIDGLDIVGTAADGDEAV
                     RKFRKLLPDAVTMDLTMPGMDGLQTISAVLAIRPDTRILVVSALADRSTAIEAIRRGA
                     RGFLYKPFDDAQLNEALTELLELH"
     gene            3107..3562
                     /locus_tag="PK144_16610"
     CDS             3107..3562
                     /locus_tag="PK144_16610"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNF67395.1"
                     /translation="MLNETQLKVFVDHATHYFSAFAQEPAIVRVPYLISHDESLVYDY
                     TGVIGVSGAFRGCVYFTAPARMMQYLLMTQGETEHSQEHCLDVVGEIANTLAGNARRE
                     LGSDFMISVPIRIAGKPEQLTLARSARSYAIPIQWRVYSAALVVSLEDG"
BASE COUNT          580 a         1321 c         1216 g          545 t
ORIGIN      
        1 tggccgtgcg gaaccgggcc gcaagcctgg gggctgcgca tcgtcctgac agcccctctg
       61 cccgcgcggg cggggggctg acgagtgcag gacagcggcg tttcaggaac ctcccgacgc
      121 gccgcgcccc ggtgccggcg aacgggtttt ccgggcagga gcgggttttc aatccacagc
      181 ttccggcttc accggaaccg tcgtccgccg ccctctgccc cgccgcgacg gatgacggcc
      241 gctgcaccgt cgcagtgcaa cagaatgcaa tccggctgcc cgatgccggg gcaatgcggc
      301 acggcatgac gccgaatcct taccgatcat tcatttatca ggcgttccgg actatgttcc
      361 ggggctgtcg gcctctggcc cgataattgc gaagtgttga tcgggatcat ggtccgcccg
      421 cccggaccct gattccggac cccaggactt ccgtcggcaa acaggaccgc atcatgaacg
      481 tcgccgcccc ctcgaccgtt ccacgccgcc gcaccggctt cggcaagtac cgcacgatcg
      541 tgctgtcgat cgccttgttt ctcgtgttcg acctcggcgt gctgatcctc aacttctata
      601 cgtcgagcga gatcagcacc gacgccgtcg gcatcaacct cgccggccgc cagcgcatgc
      661 tgtcgcagcg tctggtcaag gccctgcacg agttcggggc ggcgccggcc ggcagtaccg
      721 aagcgaccaa cgcgctgatg gaactgcgca acaccacgac gctgttcgac accacgctga
      781 gcgccttcga gcgcggcggc gagaccaccg gcggtgacgg ccgcgcggtg ctgctgcagc
      841 cggtgagcga cgccagaggc cgcgccgcgg tcgaggcggc gctgaaactc tggcagccgc
      901 tgaaaacggc gctcgatccg gcgctgcgcg gcagcgatgc ggcggcgctg cacgaggcgg
      961 tgcgcctggc gagcagcagc aacctggcgc tgctgcggct gatgaacgac ctgaccacgg
     1021 cactcgaaca ggtcgccgcc ggcaaggccg accgcctgcg cctgatccag accatcggca
     1081 tcgggcttgc gctcgcgaac ttcttcctga tcctctttca cttcatccgc cagctgcgcg
     1141 acagcgacac gcgcgccgag accgcgcggc gcgagacggt cgagatcctc gataccgtca
     1201 ccgacggcct gttcctgctc gaccgggagc tgacgatcgg cgcgcagccg tcgaagtcgc
     1261 tgcacggtct gttcggccgc agcaccctcg ccaaccagcc cttcctgccg atgctgcgcg
     1321 agatcgtctc cgagaaggtc tatcgcgagg cggcggattt cctcgacgtg ctgttccgcg
     1381 agcacgtcaa cctgaagctg gtggcgagcc tcaacccgct gcacgaggtc gaggtgctga
     1441 ttcccggccc gaacggcgaa ccgcagcgca agtacctcgg catcgagttc acgcgcgtca
     1501 ccgatgcgca ctcggcgctc gtgcacctgc tggtgacggt gcgcgacatc accgtgcgca
     1561 tcgagctggc gcgccagctc gaactgtcgc gccgtcaggc cgaggaacag atgagcctgc
     1621 tgctcggcat cctgcacgtc gagccgccgc ggctgcgcga gttcatcgac cgcgccgaca
     1681 aggcgctgca cgacatcaac gccacgctgc gccgccacgg cagcaagcac ctgtcgaagc
     1741 gcgacgaata tcgcggcgtg ctgaacgaga tcttcccgac catccacgcg ctgaagggcg
     1801 aggccggcct gctcgggctg gagttcttcg agaagctcgc gcacgacttc gaggagcgca
     1861 tcgtcgagct gcgtggcctc aagcgcgtcg gcggcgacga gctgatcggg ctcgcggtgc
     1921 agctcgaccg gctgatcggc agcgtcgagg cggtgcgcgc gatggtcgag cggctcggtt
     1981 cgctgcagca gggcttcgcg caggaagcga cggccgctgc cggggcgccg gccggggccg
     2041 cggccgaaac gccggcgatg gccccgacca gcaccgctgc gccgctgccg gagctgaccg
     2101 tcgacacctt ccgcttcctg gtcgagcgtc tggcgcagac cgagcacaag cgcgtgcagc
     2161 tgctcgcgag cggcctcgac acgctcgcgg tcagcggcgc cgagcagatc acggcgctgc
     2221 gcgaggtgct ggtgcagctg ctgcgcaacg ccgtcgcgca cggcatcgag accccggagg
     2281 agcgccacct cgccggcaag accgatgtcg gcaccatccg cctgcaggca cgcgccgatg
     2341 ccagcgccgg catcgagctg atctgccacg acgacggccg cggcctcgac cccgggcgca
     2401 tccgcgccgc cgcgctgcag agcgggcgct ggagcgagga cgagctggac gcgatgggca
     2461 cggccgggct gcgcgcgctg ctgttcgagc cgggcttcac gaccgccgcc gaagccaccc
     2521 tcgccgccgg ccgcggtgtc ggcatggacg tgatcaagca ccgcgtcgag gcgctcggcg
     2581 gcacgctgtc ggtggcctcg gaaccgggac gctactgcgc gtggcgcatc acgctgcccg
     2641 gcctgccggg actcggcgaa ctcagcttcg agacgacctg atcatgcccc ctgccctgcg
     2701 acgacagact cattgaggac aggaccatgc cggaaccgaa gccgctgacg ctgctgatcg
     2761 tcgacgattc acacatcatc cgccgccgca tcgaacgcag cgtcgccatc gacggcctcg
     2821 acatcgtcgg caccgccgcc gatggcgacg aggccgtgcg caagttcagg aagctgctgc
     2881 ccgacgccgt gaccatggat ctgaccatgc ccggcatgga cggcctgcag acgatctcgg
     2941 cggtgctggc gatccggccc gacacgcgca tcctggtcgt ctcggcgctc gccgatcgct
     3001 cgaccgcgat cgaggcgatc cgccgcggcg cgcgcggctt cctctacaag cccttcgatg
     3061 acgcgcagct caacgaggcg ctgacggaac tgctggaact gcactgatgc tgaacgaaac
     3121 ccagctcaag gtcttcgtcg accacgccac gcattacttc tcggccttcg cgcaggagcc
     3181 ggcgatcgtg cgcgtgccct acctgatcag ccacgacgag tcgctggtct acgactacac
     3241 cggcgtcatc ggggtttcgg gcgcgttccg cggctgcgtg tacttcacgg ccccggcgag
     3301 gatgatgcag tacctgctga tgacgcaggg cgagaccgag cactcgcagg agcactgcct
     3361 cgacgtggtc ggcgagatcg ccaacacgct cgccggcaac gcccggcgcg aactcggctc
     3421 cgacttcatg atctcggtgc cgatccgcat cgccggcaag cccgagcagc tgaccctcgc
     3481 ccgcagcgcc cgctcgtatg cgatcccgat ccagtggcgc gtctacagcg cggcgctggt
     3541 ggtgagtctg gaggacggct gaggcgcgat gcgatccggc tgctgatcct gtcgtgaggc
     3601 cggccaccta cgctgtggcc gtctcccccg ccctcgcagg atcgtccccg atgagcagcg
     3661 tc
//
LOCUS       DAOQIE010000354         3628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16056_length_3628_cov_9.130143, whole genome shotgun sequence.
ACCESSION   DAOQIE010000354 DAOQIE010000000
VERSION     DAOQIE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16056_length_3628_cov_9.130143"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK144_16615"
     CDS             <1..403
                     /locus_tag="PK144_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67396.1"
                     /translation="TVGKREALMRGRLKLVLLAGFAAGGCASLPAGLTPTSAEHACRA
                     VLDPPADGSDPWFRLGNALAEAGDYDAAERAYRESLRRRPDPRTRHNLALVQLQLGLT
                     GLQAAAAALPADDPARVASRAYLHELRQTLP"
     gene            597..1055
                     /locus_tag="PK144_16620"
     CDS             597..1055
                     /locus_tag="PK144_16620"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNF67397.1"
                     /translation="MRPVTVLFLGRHNAARSQIAEALLRRCGRPLFFACSAGTETGAL
                     DPLAQSVLAEIGVDISGQLAKPVENFHGRAFDCVITLCPEALAYLPELPGEYRHIHWD
                     FPDPSEVTGGEVARRRCMKRIVVQLGEHIREWVGQERRRLVANGHVAAVA"
     gene            1107..1547
                     /locus_tag="PK144_16625"
     CDS             1107..1547
                     /locus_tag="PK144_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF67398.1"
                     /translation="MQEYRHVLFATDLSPKAAEVGRHAQGLAARYGARLSFVHVIEEV
                     NVSAGYELMPLLPEMPDEALTRESKAALARLAAELGLPEAVQTVLTSVSTKAGILDTA
                     AELGVDLIVVGSHGRHGLALLLGSTADAVLHGARCDVLAVRLPD"
     assembly_gap    1562..1661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1662..2641)
                     /locus_tag="PK144_16630"
     CDS             complement(<1662..2641)
                     /locus_tag="PK144_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF67399.1"
                     /translation="MGMLDSVGIKLVAALQGGGAHGAYTWGVLDRLLDEPAIRFTGFS
                     GTSAGAMNAVVCAHGLALGGRQGAQAALARFWSAVADASRLTPAQPGPLDRLLGDDSL
                     HWSPGFRSFIALTRVLSPYQFNPLNVNPLRDILEAQIDFERLRAPAAPRLWVAATRVR
                     TGTVRLFTNAELSIDAVLASACLPSLHQAVVIDGELYWDGGYSANPPVRVPAESGAAN
                     DLLLVLLAPLEREGPFHTAPEILGRASEIAFHATFIQEMQGLAERAAAPIWQRYSDPA
                     ARRRYHLLAAGPELTELPQPSKLNADRAFLDRLFEAGRSRAEAWLAADGAA"
     gene            complement(2755..3513)
                     /locus_tag="PK144_16635"
     CDS             complement(2755..3513)
                     /locus_tag="PK144_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF67400.1"
                     /translation="MSRSTSRPPVLLAALAQLLALFAVWALQVGLIRLFGRPPVDAGV
                     LLFVQGLLAAALGLRFGLPRWWIPIQIVFLPLAWGALTLALPPLLWLALAAVVFLIFR
                     NSALQRVPLYLSGPDAWAALAQRLPRADARVIDLGCGLGGPLLGLARQCPQARIEGIE
                     SAPLPWLCAWLRCLREPRILVRYGDFGALDLGAYDLVFCFLSPQPMLTLWLKARAEMR
                     AGSLFASLEFPVPDLPADEQLQLPGGRTLYLWRL"
BASE COUNT          559 a         1291 c         1243 g          435 t
ORIGIN      
        1 caccgtcggc aagcgcgagg cgctgatgcg aggccggctg aagctcgtgc tgcttgccgg
       61 cttcgccgct ggcggctgcg cgtcgctgcc ggcgggtctg acgccgacct cggccgagca
      121 tgcgtgccgg gccgtgctgg acccgcccgc cgacggcagc gacccgtggt tccggctcgg
      181 caacgcgctc gccgaagccg gcgactacga cgccgccgaa cgcgcctacc gcgaatccct
      241 gcgccgccgg cctgatccgc gcacccggca caacctggct ctggtgcagt tgcagctcgg
      301 cctcaccggc ctgcaggccg ctgccgccgc gctgccggcc gacgacccgg cccgggtcgc
      361 cagccgcgcc tatctgcacg agctgcggca gaccctgccc tgatccggcc ccgggcgcgc
      421 ccggcggcaa tcgaggattt gcctgacgcc cgtcaatgca cggtcacgca tcagcggcat
      481 gctgccgccg tgcgctgccc atggcgcacc caccaccaga gcacgatgtc ccggttcagg
      541 gacacgccat aacaagcggt cgaggatcga ccgcaacaag atcgaggcaa ggatccatgc
      601 gtcccgtcac cgtcctgttc ctgggccggc acaatgctgc tcgctcgcag atcgccgagg
      661 cgctgctgcg ccgctgcggc cgtccgctgt tcttcgcctg cagtgccggc accgaaaccg
      721 gcgcgctcga cccgctcgcg cagtcggtgc tggccgagat cggcgtcgac atttccggcc
      781 agctcgccaa gccggtcgag aactttcacg gccgggcctt cgactgcgtg atcacgctct
      841 gcccggaggc cctggcctat ctgccggaac tcccgggcga gtaccgtcac atacactggg
      901 attttcccga tccgtccgaa gtcaccggcg gcgaggtcgc gcggcggcga tgcatgaagc
      961 gcatcgtcgt gcagctcggc gagcacatcc gcgagtgggt cggccaggaa cgccggcgcc
     1021 tggtggcgaa cggccacgtc gcggccgtcg cctgaggccc gcggacggat cggaaacccc
     1081 caccgatctg cccggaggac cgtgccatgc aggagtaccg ccacgtactg ttcgccaccg
     1141 acctgtcgcc gaaggccgcc gaagtcggcc ggcacgcgca aggtctggcc gcccgctacg
     1201 gcgcgcgcct gtcgttcgtg cacgtcatcg aggaagtcaa cgtcagtgcc ggctacgagc
     1261 tgatgccgct gctgccggag atgcccgacg aggcgctgac ccgcgaatca aaggccgcac
     1321 tcgcccggct ggctgccgaa ctcggcctgc cggaggccgt gcagacggtg ctgacctcgg
     1381 tcagcaccaa ggccggcatc ctcgacaccg ccgccgaact cggcgtggac ctgatcgtgg
     1441 tcggcagcca cggtcgccac gggcttgcgc tgctgctcgg ctcgaccgcc gatgccgtgc
     1501 tgcacggcgc ccgctgcgac gtgctcgccg tgcgcctgcc ggactgaatc acgcggcggg
     1561 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcggcgccg tccgcggcga
     1681 gccaggcctc ggcccggctg cgaccggcct cgaacagccg gtcgaggaag gcgcgatcgg
     1741 cgttgagctt gctcggctgc ggcagctcgg tcagttcagg gccggcggcg agcaggtgat
     1801 agcgccggcg ggccgccgga tcgctgtaac gctgccagat cggcgcggcc gcgcgttcgg
     1861 cgaggccctg catttcctgg atgaacgtgg cgtggaaggc gatctcgctg gcgcgaccga
     1921 ggatctcggg cgcggtgtgg aacggccctt cgcgttcgag cggcgccagc agcaccagca
     1981 gcaggtcgtt ggccgcgccg ctctcggccg gcacgcgcac cggcggattg gcgctgtagc
     2041 cgccatccca gtacagctcg ccgtcgatca cgacggcctg atgcagcgac ggcaggcagg
     2101 ccgaggccag caccgcgtcg atcgacagtt cggcattggt gaacagccgc acggtgccgg
     2161 tacgcacgcg cgtcgccgcc acccacagcc gcggtgcggc cggggcgcgc agccgctcga
     2221 agtcgatctg cgcttcgagg atgtcgcgca gcggattcac gttcagcgga ttgaactggt
     2281 acggcgacag cacgcgggtc agcgcgatga aggagcggaa gcccggcgac cagtgcaggc
     2341 tgtcgtcgcc gagcagccgg tcgagcggac cgggctgggc cggcgtcagg cgcgaggcat
     2401 cggcgaccgc cgaccagaac cgcgccagcg ccgcctgtgc gccctggcgc ccgccgagcg
     2461 ccaggccgtg cgcgcagacc acggcgttca tcgcgccggc gctggtgccc gagaaccccg
     2521 tgaagcggat cgccggttca tcgagcaggc gatccagcac cccccaggtg tacgcaccgt
     2581 gcgctccgcc gccctgcagg gcggccacga gtttgatgcc gacgctgtca agcatgccca
     2641 tcgactgatc tccgcaattc cgaccgcctg ttgcggccac gatcacactg accgtgtccg
     2701 cgccacgatg ttgcatcgca acaagcatag acggggcagc gcggggtatc gcgtttacaa
     2761 acgccagaga tacagcgtgc gtccgcccgg caactgcagc tgctcatcgg cgggcaggtc
     2821 cgggaccggg aattcgaggc tcgcgaacag gctgccggca cgcatctcgg cacgcgcctt
     2881 cagccacagc gtcagcatcg gctgcggcga caggaagcag aacaccagat cgtaggcacc
     2941 gagatcgagc gccccgaaat cgccgtagcg cacgaggatg cgcggctcgc gcaggcagcg
     3001 cagccaggcg cagagccacg gcagcggcgc cgattcgatg ccctcgatgc gcgcctgcgg
     3061 gcactggcgc gccagcccga gcagcgggcc gccgagtccg cagccgagat cgatgacgcg
     3121 cgcatcggcc cgcggcagac gctgcgccag cgccgcccag gcatccgggc cggaaagata
     3181 caagggcacg cgctgcagcg cgctgttgcg aaagatcagg aataccacag ccgcgagtgc
     3241 cagccacagc agcggcggca gcgccagcgt cagcgccccc caggccagcg gcaggaacac
     3301 gatctggatc ggaatccacc agcgcggcag cccgaagcgc aggccgaggg cagcggccag
     3361 caggccctgc acgaacagca ataccccggc atcgaccggc ggacgaccga acagccggat
     3421 caggccgacc tgcagcgccc agaccgcaaa cagcgccagc agctgggcaa gcgcggcgag
     3481 caggaccggc ggtcgcgagg tgctgcgact cacggcgtcg ccgggcgcgc cggcaccaga
     3541 cccggcggca gcggcggcac gatcgaaggc tggccggcgg ggggtgcggc cggacgcggc
     3601 gccggggcgg ccgcaggcgg tgcggccg
//
LOCUS       DAOQIE010000355         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16063_length_3627_cov_5.983763, whole genome shotgun sequence.
ACCESSION   DAOQIE010000355 DAOQIE010000000
VERSION     DAOQIE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16063_length_3627_cov_5.983763"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PK144_16640"
     CDS             <1..1381
                     /locus_tag="PK144_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNF67401.1"
                     /translation="SVRDVMDNCPAIANADQVDTDGDGQGDACDEDDDNDGIADRSDF
                     APLSAQPPTDSDGDGIADAWEMAHFGNLTSVDATSDHDRDGITDVKEFAARGNPRVAA
                     LHDSLSLVPDQTQDGVAELAMVRMFEHYARLDVVNGKTGEVLSTGRFYFNDYDPLGLV
                     YMGRGSDSGMMEFAALAVDSYRNKAFIEIRTATGALVRSFELDSLPVTQMLQYESIAG
                     QKRFVLPGYDAGRGQMVATIIDPAGRVERSVDFGTGSTLVDFRTFQRQGEAWAVAGMQ
                     QAGGQFVLRLRSLVNDQQNQVVLPAGYALAAIDLIEASTGHEGPYLVASQPRLVNRES
                     EAGIRQFYLTSTFALAKTHTLPALRSLLAHGVFTQPDPVFGFVNFIDAEGYAAQWVAK
                     VQYGGGAYRFRYLPAQEIRIAYECQGTASICVASLRPDGRLIGFDAANQRSWVLNSKE
                     QGTYQLTGN"
     gene            1619..2593
                     /locus_tag="PK144_16645"
     CDS             1619..2593
                     /locus_tag="PK144_16645"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNF67402.1"
                     /translation="MDEANRYSFGITRLRPGASRQGRSVHIACGLVAMLMAVDMATLV
                     TAQVPGASKVSGTDAGGPVVTQPVPEPLGRYALFGVAESPRDTPPPAAALKPSARPQL
                     RLTGTLAGAGHGLALISVGGQAEQAYPVGGTLASGWRLTAVFADRVQIAGFGESIEVP
                     MPAGESALARTSAPRDPAPAVSGVPAFPAAADDHEFSEDDPQAGSARASGRQERTPVD
                     ARPIARRLRGAPQPDAALRQLAGFDPVSLADHHGYGLRLRLGPESYLLTRAGLREGDV
                     IVAIGGVLLQDGAQARQLAMAALEATELELRLLRENEELTMDFDLGRN"
     gene            2699..>3627
                     /locus_tag="PK144_16650"
     CDS             2699..>3627
                     /locus_tag="PK144_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNF67403.1"
                     /translation="MTLNLKDADIGTLVQAVSEITGRNFIVDPRIKARVTVISSRPMD
                     ERDVYAVFLSVLAVHGFAAIPGDKVVKIVPEADAKQETVPTVEDGRRGVEADQIVTRV
                     IQVRNTAAAQLVPLIRPLIPAQGHLAALPGPNMLVISDKAANVERVGELIARFDQAAR
                     EDTEVVTLQNATAADLLPVIAQLEAKTGEGGMTPVRVLADERTNSLILKGEQLARARL
                     RQLIERLDAPVAGRGKTQVVFLRYAQAKTLATALQGLAKQLAGAARSAEPAGGGGGAV
                     SIEAEENSNALLLTAPPTALRSLLEVIAQLDVRR"
BASE COUNT          609 a         1115 c         1294 g          609 t
ORIGIN      
        1 cagcgtcagg gatgtaatgg ataactgccc ggcgattgcc aatgccgatc aggttgatac
       61 ggatggcgat ggccaggggg atgcctgtga tgaggatgat gacaatgatg gcatcgctga
      121 tcgcagcgac tttgcgccac tttccgccca gccaccgacc gatagcgatg gcgacggtat
      181 cgcggatgca tgggaaatgg ctcatttcgg aaatctgacc tcggtcgatg cgacatccga
      241 tcatgatcgt gatggcatca cggacgtgaa ggaattcgct gcccgcggca acccgagggt
      301 ggcggcgctg cacgattccc tgtctcttgt tccggatcag actcaggatg gtgtggctga
      361 actggccatg gtcaggatgt tcgagcatta tgcccgtctg gacgtggtca acggcaagac
      421 cggggaagtg ttgagcacgg ggcgcttcta tttcaatgac tacgacccgc tggggctggt
      481 gtacatgggg cgcggcagtg acagcggcat gatggagttt gctgcgctgg ccgtggattc
      541 gtatcgaaac aaggccttca ttgaaatcag gactgccacc ggcgcactgg tcaggagttt
      601 cgaactcgac agtctgccgg tgacgcagat gctgcagtac gagtcgattg ccggacagaa
      661 gcgtttcgtc ctgccgggct acgatgcggg caggggacag atggtggcga ccatcatcga
      721 tcctgccggg cgggtcgagc gcagcgtcga tttcggaacg ggcagcacgc tggttgattt
      781 caggactttc cagcgtcagg gagaagcctg ggccgtggcc ggcatgcagc aggcgggcgg
      841 gcagttcgtg ctgcgcctgc gcagtctggt gaatgatcag cagaaccagg tggtgctgcc
      901 ggccggctat gcgcttgcgg ccatcgatct gatcgaagcc agcaccgggc atgaagggcc
      961 ttatctggtg gcttcgcagc cgcggctggt caaccgtgaa tccgaagcgg gtatccggca
     1021 gttttatctg accagcacct tcgcgctggc caagacccac acgctgccgg ccttgcgctc
     1081 gctgcttgcg catggcgtgt tcacccagcc tgacccggtg ttcggtttcg tgaacttcat
     1141 tgatgccgaa ggctatgccg cgcagtgggt ggcaaaggtg cagtatggcg gcggtgcata
     1201 ccgcttccgg tatctgccgg ctcaggaaat caggatcgcc tacgaatgtc agggcacggc
     1261 ttcgatctgt gtggcttccc tgcgtcctga tggccggctg atcggtttcg atgcagccaa
     1321 ccagcgctca tgggtgttga acagcaagga acagggcacg tatcagctga ccggcaactg
     1381 agcgcctcga agtaacaggc aaaggcaggg gagatcggca ggcaggcggg cttgcgggat
     1441 gcgcaggacg cctgcctgca ggacgccggt gatgcggtgc cggggtttgc ggccggcggc
     1501 ggggactgcg cacggatggt ggatgcaagg ctgcagccgg ccgcaccggc ggagcgtccg
     1561 ggaaaccggg cggttccggc ctgcggggcc cggcatcgat gcacgggaac ggacggatat
     1621 ggacgaagcg aatcggtaca gtttcgggat cacccgcttg cggccgggag cttcccggca
     1681 ggggcgttcc gtacacatcg cctgtggact ggtggcgatg ctgatggccg tggacatggc
     1741 cacgctcgtg acggcgcaag tgccgggcgc atcaaaggtt tccggcaccg atgcgggcgg
     1801 ccccgtcgtg acgcagccgg tgcccgagcc gctgggtcga tacgcgctgt tcggcgtggc
     1861 cgaaagcccc cgggacacgc cgccacccgc tgcagcgctc aagccgtccg cccggcctca
     1921 gctgcgtctg accggaacgc tggccggtgc cgggcacgga ctggccttga tctcggtcgg
     1981 cggacaggcc gaacaggcct atccggtcgg cgggaccctg gccagcggat ggcgattgac
     2041 cgccgtgttc gcggaccggg tgcagatcgc cggcttcggc gaaagcatcg aggtgcccat
     2101 gccggcaggc gaatccgccc tggcgcggac gagcgcaccg cgcgatccgg ccccggcagt
     2161 ttccggggtg ccggcgttcc cggcagcggc ggacgatcac gagttttcag aggacgaccc
     2221 gcaggccggg tccgcccgcg catccggccg gcaggaacgc acgccggtgg atgcgcgacc
     2281 gatcgcgagg cgcctgcggg gtgcccccca gccggatgcg gccctgcgcc agctcgccgg
     2341 tttcgatccg gtctcccttg cagatcatca cggctatggc ctgcgcctgc ggctgggacc
     2401 cgaatcgtat ctgctgaccc gtgccggcct gcgcgaaggc gatgtcatcg tggccatcgg
     2461 cggggtgctg ctgcaggacg gggcacaggc gcgtcagctg gcgatggcgg cactcgaggc
     2521 gacggagctc gaactgcggc tcctgcgcga gaatgaggaa ctgacgatgg acttcgacct
     2581 cgggcgcaac tgaatgcctg tcgggcgagg aaacggacgg gtcatgtttc gtggatttct
     2641 gcatggcgcc ttcgcaggcg tcgtcctgtg cgcggcagcg actgcgcagg ccggccccgt
     2701 gacgctgaat ctcaaggatg ccgacatcgg cacgctggtc caggcggttt ccgaaatcac
     2761 cggcaggaac ttcatcgtcg atccgcgcat caaggcgcgc gtcaccgtga tttcctcgcg
     2821 gccgatggac gagcgggatg tctacgcggt gttcctgtcg gtgctggcag tgcacggctt
     2881 tgcggcgatt cccggcgaca aggtcgtcaa gatcgtgccc gaggccgatg ccaagcagga
     2941 aacagtgcct accgtcgagg acggacgccg cggcgtcgag gccgaccaga tcgtcacgcg
     3001 cgtgatccag gtacgcaaca cggccgccgc gcagctcgtg ccgctgatcc ggccgctgat
     3061 tccggcccag gggcatctgg cggccttgcc cggccccaac atgctggtga tctccgacaa
     3121 ggctgccaac gtcgagcgtg tcggcgaact gatcgcccgc ttcgatcagg ccgcccgcga
     3181 ggacaccgag gtcgtcacgc tgcagaacgc cacggctgcc gacctgctgc cggtgatcgc
     3241 gcagctggag gcgaagacgg gcgagggtgg catgacgccg gtgcgggtcc tggccgacga
     3301 gcgcacgaac agcctcatcc tcaagggcga gcagctcgcc cgcgcacgcc tgcggcaact
     3361 gatcgaacgg ctggatgccc cggttgccgg ccggggcaag acgcaggtcg tgttcctgcg
     3421 ctatgcccag gccaagacgc tggcgacggc gctgcagggc ctggccaaac agctggccgg
     3481 tgcggcccgc agtgccgagc cggcgggcgg cggcggcggg gcggtcagta tcgaagccga
     3541 ggagaactcc aatgcgctgc tgctgacggc accgccgacg gcgctgcgct cgctgctcga
     3601 agtcatcgcg caactcgacg tgcgccg
//
LOCUS       DAOQIE010000356         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16138_length_3618_cov_8.309009, whole genome shotgun sequence.
ACCESSION   DAOQIE010000356 DAOQIE010000000
VERSION     DAOQIE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16138_length_3618_cov_8.309009"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK144_16655"
     CDS             <1..222
                     /locus_tag="PK144_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNF67404.1"
                     /translation="GCEGATLCTTRGALHSPAYPAEFRQQMIELVRSGKTPEQLAEEF
                     EPTAQTIRNWMAQAGRDAGTAHPSAELST"
     gene            252..746
                     /locus_tag="PK144_16660"
     CDS             252..746
                     /locus_tag="PK144_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNF67405.1"
                     /translation="MVFGDYQDPRSLALQRIQTAHVLDEPARRLPGFDLDAWLAQRDF
                     DLPLGDDIELHAVLSRELEVLLRERPLAPDQQLEAAGDHEFVLRARVADTMRLRWWLL
                     GFGDRITVLGPDRLREEMAATVRRMAAVYAGDAARDSMETSRTPATDMAVSAARNVQT
                     AAHR"
     gene            770..1768
                     /locus_tag="PK144_16665"
     CDS             770..1768
                     /locus_tag="PK144_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNF67406.1"
                     /translation="MDILSDDPVTDRIRASLLAGAIGDALGAPVEFHTIGRIRARHGV
                     HGLRDYVPAYGRRGAVTDDTQMTLFTAEGLLEALRAPDSAPGSAIDAAYRRWLRTQRT
                     PFPGAGRESGLLAEAALWATRAPGHTCLSALANPGRGLGERADNNSKGCGGVMRVAPI
                     GLCRRVGDPFALASAAARLTHNHPSGYLAAAAFALLVRRLLDGEALRDALAVTLDRLA
                     GEPGHEETTAALLQARALAEAGGPATPERLMQLGGGWVAEEALAIALWCALVAPDLES
                     ALLLAINHDGDSDSTGSLTGNLLGAALGMAAIPARWLADLELRECIVRVADELAAA"
     gene            1891..2790
                     /locus_tag="PK144_16670"
     CDS             1891..2790
                     /locus_tag="PK144_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF67407.1"
                     /translation="MFPPSASPAHRAFVAAMPALFVLLWSTGFIGAKLGLPHAEPFTF
                     LAIRMLIATALLAVLAFATAAPWPRTPAEAARIALAGLLNQAGYLGGVFYAIGTGLPA
                     GITALIVGFQPLLTAALAGPLLGERVTRAQWAGLGLGLLGIVLVLAGREHAAGGQITP
                     AGIASALLALLTITFGTVWQKRHCGGMDLRTGGVVQYGASALALGALAFAFETRIVDW
                     TPAFAFALGWLVLVLSVGAVGLLYTLIRRGEVARVGSLFYLTPPVTALMAWAMFGERL
                     GAVAILGMALVAGAVWLANRGPR"
     gene            2963..3487
                     /locus_tag="PK144_16675"
     CDS             2963..3487
                     /locus_tag="PK144_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNF67408.1"
                     /translation="MTRIRAVLFDWGNTLMRDLPGQHGPMRDWPHVEALPGAPEALEA
                     LRAQGLRLALATNAADSGPADIRAALARCGLSAALEQIFCARELGLAKPARAFYGACL
                     AGLDLPAEAVAMVGDDWHGDVQGALHAGLAAVWLQARRSLLPPVPDGACVTTIAALGE
                     LPELFTHWPPAETG"
BASE COUNT          458 a         1351 c         1299 g          510 t
ORIGIN      
        1 ggctgtgagg gcgcgacact ttgcactacc aggggagcgt tgcacagccc tgcgtaccct
       61 gccgagttcc ggcagcagat gatcgaactg gtgcgcagcg gcaagacgcc ggagcagctt
      121 gccgaggagt tcgagcccac ggcgcagacg atccgtaact ggatggcgca ggccgggcgc
      181 gatgccggca cggcgcaccc aagcgctgaa ctgtctacgt aagcggggca acttcacttg
      241 cgtatctcgt cgtggtgttc ggcgattacc aggacccgcg ctctctggcc ctgcagcgca
      301 tccagaccgc gcacgtgctc gatgagccgg cgcgccggct gccgggattc gatctcgatg
      361 catggctggc gcagcgcgat ttcgatctgc cgctcggcga tgacatcgaa ctgcacgccg
      421 tcctctcccg cgagctggag gtgctgctgc gcgaacggcc gctggcgccg gatcagcagc
      481 tggaagcggc cggcgaccat gaatttgtcc tgcgcgcgcg cgttgccgac acgatgcgcc
      541 tgcgctggtg gctgctcggt ttcggcgatc gcatcacggt gctggggccg gacaggctgc
      601 gtgaggaaat ggcggcaacg gtccggcgca tggccgcggt ctatgcaggg gacgcggcac
      661 gggacagcat ggaaacaagc cgcaccccag cgacggacat ggccgtttct gctgcgagga
      721 acgtgcagac tgccgcacat cgatgacctg cccgaggaac cctgcgccca tggacatcct
      781 gagcgatgac cccgtgaccg accgcatccg cgccagcctg ctcgccggcg ccatcggcga
      841 tgcgctcggc gcaccggtcg aattccatac gatcggccgc atccgcgccc gccacggcgt
      901 ccacggcctg cgcgactacg tgccggccta cggccgtcgc ggcgcggtca ccgacgacac
      961 gcagatgacg ctgttcacgg ccgagggcct gctcgaagcg ctgcgggcgc ccgacagcgc
     1021 cccgggcagc gcgatcgacg ctgcctaccg ccgctggctg cgcacgcagc gcacgccgtt
     1081 tcccggcgcc gggcgcgaga gcggcctgct cgccgaagcg gcactctggg cgacgcgcgc
     1141 ccccggccat acctgcctgt cggcactcgc aaaccccggg cgcggcctcg gcgagcgggc
     1201 cgacaacaac agcaagggct gcggcggggt catgcgcgtc gcaccgatcg gcctctgccg
     1261 ccgcgtcggg gacccgttcg cgcttgcctc ggccgctgcg cgcctgaccc acaaccaccc
     1321 gagcggctac ctcgccgccg cggccttcgc gctgctggtg cgccggctgc tcgacggcga
     1381 agccctgcgc gacgcgctcg ccgtgacgct cgatcgcctc gccggcgaac ccggccacga
     1441 ggaaaccacg gccgcactgc tccaggcccg ggcgctcgcc gaggccggcg gcccggccac
     1501 gcccgagcgc ctgatgcagc tcggcggcgg ctgggtcgcc gaggaagcgc tggcgatcgc
     1561 gctctggtgc gcactggtcg cgcccgatct cgaatcggcg ctgctgcttg cgatcaacca
     1621 cgacggcgac agcgacagca ccggctcgct gaccggcaac ctgctcggcg ccgcgctcgg
     1681 catggccgcg atcccggcgc gctggctggc ggacctcgaa ctgcgcgagt gcatcgtgcg
     1741 cgtggcggac gaactcgccg cggcctgagc cccgcgccgc gcgtccggcg gcctgtaagc
     1801 cctgcatgcc ggcgatgcag ccggcgcagg gccgggccgg ggagggctcg gctaagctgc
     1861 gcgcccccgt tcctcccgcc ggattcccgc atgtttcccc cgtccgcatc cccggcgcat
     1921 cgcgccttcg tcgcggcgat gcccgcgctg ttcgtgctgc tgtggagcac cggcttcatc
     1981 ggcgccaagc tcgggctgcc gcatgccgag ccgttcacct tcctcgccat ccgcatgctg
     2041 atcgccaccg cgctgctcgc cgtgctcgcc ttcgcgaccg ccgcgccgtg gccgcgcaca
     2101 ccggccgagg cggcgcgcat cgcgctcgcc gggctgctga atcaggccgg ttatctcggc
     2161 ggcgtgttct acgcgatcgg caccggcctg ccggccggca tcaccgcgct gatcgtcggc
     2221 ttccagccgc tgctgaccgc tgcgctcgcc gggccgctgc tcggggagcg ggtcacgcgt
     2281 gcgcagtggg cggggctcgg gcttggcctg ctcggcatcg tgctggtgct cgccgggcgc
     2341 gagcacgcgg ccggcgggca gatcacgccg gccggcatcg cctcggcgct tctggcgctg
     2401 ctgaccatca ccttcggcac cgtctggcag aagcgccact gcggcggcat ggacctgcgc
     2461 accggcggcg tcgtgcagta cggcgcctcg gcgctcgcgc tcggcgcgct ggccttcgcc
     2521 ttcgagacgc gcatcgtcga ctggacgccg gccttcgcct tcgcgctcgg ctggctggtg
     2581 ctggtgctgt cggtcggcgc ggtcgggctg ctgtatacgc tgatccgccg cggcgaggtc
     2641 gcgcgcgtcg gcagcctgtt ctacctgacg ccgccggtga cggcgctgat ggcctgggcg
     2701 atgttcggcg aacgcctcgg ggccgtggct atcctcggca tggcgctggt ggccggcgcc
     2761 gtgtggctcg ccaaccgcgg accgcgctga tgccgatgca gaagccccgg catcacggcg
     2821 cgggtcagcg gatcggcacg gcgagccggg gcgtgacggg gccgggctga acttgcgccc
     2881 gtccccgcgg gtacgcgggt aaaggcgggg aaatttcccc gccggatcgg gatcgggatc
     2941 ggcagggcag gggcgacggg cgatgacgag aatccgggcg gtgctgttcg actggggcaa
     3001 cacgctgatg cgcgacctgc ccggccagca cgggccgatg cgcgactggc cgcacgtcga
     3061 ggcgctgccc ggcgcgcccg aagcgctcga agcgctgcgc gcgcagggcc tgcggctggc
     3121 gctcgcgacc aacgccgccg actccggccc cgccgacatc cgcgccgcgc tggcgcgctg
     3181 cgggctgtcg gcggcgctcg aacagatctt ctgcgcccgc gaactcggcc tcgccaaacc
     3241 ggcccgcgcc ttctacggcg cctgcctcgc cgggctcgac ctgccggccg aggcggtggc
     3301 gatggtcggt gacgactggc acggcgacgt gcagggcgcg ctgcacgcgg ggctcgccgc
     3361 cgtctggctg caggcccggc gcagcctgct gccgccggtg ccggacggcg cgtgcgtgac
     3421 cacgatcgcc gcgctcggcg aactgccgga gttgttcacg cactggccgc cggcggaaac
     3481 cggctgaggc cagcgcgcgc cggcctcatc catccggacc ggctacgctc gccggacatc
     3541 ccccgaccgg aagggagtcc gcgatgcccg tcaagccgcg cgtcttcatc agctacagcc
     3601 acgattcgga tgcccaca
//
LOCUS       DAOQIE010000357         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16260_length_3603_cov_5.262401, whole genome shotgun sequence.
ACCESSION   DAOQIE010000357 DAOQIE010000000
VERSION     DAOQIE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16260_length_3603_cov_5.262401"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2489)
                     /locus_tag="PK144_16680"
     CDS             complement(<1..2489)
                     /locus_tag="PK144_16680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNF67409.1"
                     /translation="MDSAPPPPDPIAATAAAQAAAALLDDALLFDLEATPDGRILAIG
                     AEFRGQCFLREGRLQVGAVLAELAGFAHGARLVLGHNLLDHDWPLLRAQAPQAGLLRL
                     PVLDTLYLSPLAFPENPYHALVKDYKLVRDSVNDPVADARLAGRVFLEQVAAFAARMQ
                     GGPDWADLLRYAWTDAAAGRDGLSPTAFLWLWERLGGRPPADPGALIERLIDARTCRS
                     RRSAALQPLLMQPGLRPLLGYLVTWLMVAGGNSVLPAWLRHRFPALPEAVRRLRDEPC
                     SDPACAWCRDAHDPRGQLERWFGFPDFRAEPATAEGGSLQRAIVAAGLGHGSLLGILP
                     TGGGKSLCYQVPALARYRNRGALTVVISPLRALMKDQVDGLNRRVGFELCGALYGDLT
                     PPERGALIERVQLGDIAVLYVAPEQFRNASFRSLLESREIGAWVFDEAHCLSQWGHDF
                     RPDYLYCARFIREFGERHKLPLAPVSAVTATAKPDVIDEIRRHFREELGLELTLYAGG
                     VERDNLRFEVRAVTSASKPAQVLQLLRERLEPAPGAAIVYCAKQDSTVEFARFLDSEG
                     WAAEAFHGGMKAPDKRSVQERFLAGELRVICATNAFGMGIDKPDVRLVIHADAPSSLE
                     HYLQEAGRAGRDREPADCVLLYAPEDIERQFRWGARSRLSRQDIAGILRALRRLQQKR
                     DADEPVVVTAGELLRSDSLLTGFETEDADTRVRTALAWLEKAQLVERNENRTGVFQGR
                     PSVGSMAEARAKLDALDLAPRARRRWEAVLAVLLQTDADDGLSVDTLAQLPALRPGPD
                     EKPVPVLRLLDDMHRAGLLQLGLQLTAWLRP"
     gene            2588..3361
                     /locus_tag="PK144_16685"
     CDS             2588..3361
                     /locus_tag="PK144_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HNF67410.1"
                     /translation="MNPPKIYDRPEAVWAHTPDRQPFLYMADGLLSLHFEAASVQSRM
                     DPAAPARLTLGYTRTMMGAALLLLGPVPGRIAMIGLGGGSLVKYCRQHLPEAVIEVAE
                     ISAEVIALRERFCVPADSDHLRVLCTDGAAFVATAGAGEWDLLLVDGFGPGGQPPQLG
                     SQRFYDDCRAALGADGLLVANLAGSDPRLDLYLSRLETAFGRVLNLPAADSDNRIAIA
                     GRGLATADGRLCARLKRLAPQHDLDLGRTCAALLDARRA"
     gene            3513..3603
                     /locus_tag="PK144_16690"
     tRNA            3513..3603
                     /locus_tag="PK144_16690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3547..3549,aa:Ser,seq:tga)
BASE COUNT          521 a         1329 c         1273 g          480 t
ORIGIN      
        1 ttcggtcgca gccaggcggt gagctgcagg ccgagctgca acaggccggc acggtgcatg
       61 tcatcgagca ggcgcagcac cggcaccggc ttctcgtccg gcccggggcg cagcgccggc
      121 agttgcgcga gcgtatcgac gctcaggccg tcgtcggcat cggtctgcag cagcacggcg
      181 agcaccgcct cccagcgccg gcgcgcgcgc ggcgcgagat cgagcgcatc gagcttcgcg
      241 cgcgcctcgg ccatcgagcc gaccgagggc cggccctgga acacgccggt gcggttctcg
      301 ttgcgctcga ccagctgtgc cttctccagc caggcgagcg cggtgcgcac gcgggtgtcg
      361 gcatcttcgg tctcgaagcc ggtgagcagg ctgtcgctgc gcagcagctc gccggcggtg
      421 acgacgaccg gctcgtcggc atcgcgcttc tgctgcaggc ggcgcagcgc gcgcaggatg
      481 ccggcgatgt cctgccggct caggcggctg cgcgcgcccc agcgaaactg ccgttcgatg
      541 tcctcgggcg cgtacagcag cacgcagtcg gccggctcgc gatcgcgccc ggcgcggccg
      601 gcctcctgca ggtagtgctc cagcgaggac ggcgcatcgg cgtggatgac gaggcgcacg
      661 tcgggcttgt cgatgcccat gccgaaggca ttggtcgcgc agatcacgcg cagctcgccg
      721 gcgaggaagc gctcctgcac cgaacgcttg tccggcgcct tcatgccgcc gtgaaaggct
      781 tcggccgccc agccctcgct gtcgaggaag cgggcgaact cgacggtgct gtcctgcttc
      841 gcgcagtaga cgatcgccgc acccggcgcg ggttcgaggc gctcgcgcag cagctgcagc
      901 acctgcgccg gcttgctggc actcgtgacc gcgcgcacct cgaagcgcag gttgtcgcgc
      961 tcgacgccgc cggcgtacag cgtcagctcc agcccgagtt cctcccggaa gtggcggcgg
     1021 atctcgtcga tcacgtccgg cttggcggtg gcggtgacgg cgctgaccgg cgccagcggc
     1081 agtttgtgcc gctcgccgaa ctcgcggatg aagcgcgcgc agtacaggta atcgggccgg
     1141 aagtcgtggc cccactgcga caggcagtgc gcctcgtcga acacccaggc gccgatctcg
     1201 cggctttcga gcaggctgcg gaagctggca ttgcggaact gctcgggcgc gacgtacagc
     1261 acggcgatgt cgccaagctg cacgcgctcg atcagcgcgc cgcgctcggg cggcgtcagg
     1321 tcgccgtaca gcgcgccgca cagctcgaaa ccgacgcgcc ggttcaggcc atcgacctga
     1381 tccttcatca gcgcgcgcag cggcgagatc accaccgtca gcgcgccgcg gttgcggtaa
     1441 cgcgccagcg ccggcacctg atagcacagc gacttgccgc cgccggtcgg caggatgccg
     1501 agcagcgagc cgtgcccgag cccggcagcg acgatcgcgc gctgcaggct gccgccctcg
     1561 gccgtggccg gctcggcgcg gaagtccggg aagccgaacc agcgttcgag ctgcccgcgc
     1621 gggtcgtgcg cgtcgcgaca ccaggcgcag gccgggtcgc tgcagggctc gtcgcgcagg
     1681 cggcgcacgg cctccggcag cgccggaaac cggtgccgca gccaggccgg cagcaccgaa
     1741 ttgccaccgg cgaccatcag ccaggtgacg agataaccga gcagcggccg cagccccggc
     1801 tgcatcagca gcggctgcag cgccgccgag cgccggctcc ggcaggtgcg ggcgtcgatc
     1861 aggcgctcga tcagcgcgcc gggatcggcc ggcggccggc cgccgaggcg ctcccagagc
     1921 cacaggaaag ccgtcggcga caggccgtcg cgcccggccg ccgcatcggt ccacgcatag
     1981 cgcagcagat cggcccagtc cggcccgccc tgcatgcgcg cggcaaaggc cgcgacctgt
     2041 tcgaggaaca cccgaccggc cagccgggca tcggcaaccg ggtcgttgac gctgtcgcgc
     2101 accagcttgt agtccttgac cagcgcgtga tacgggtttt ccgggaaggc gagcggcgac
     2161 agatacagcg tgtcgagcac cggcaggcgc agcagcccgg cctgcggcgc ctgtgcgcgc
     2221 agcagcggcc agtcgtgatc gagcaggttg tgcccgagca cgagccgcgc accgtgcgcg
     2281 aacccggcca gctccgccag cacggcaccg acctgcagcc ggccttcgcg caaaaagcac
     2341 tggccgcgga attcggcgcc gatcgcgagg atgcgcccgt ccggcgtggc ttcgagatcg
     2401 aagagcagcg cgtcatcgag cagcgcggct gccgcctgtg cggcggcggt ggcggcaatc
     2461 gggtccggag gcggcggggc gctgtccatg tgcacgcggg ctcgggtagc ggcctgaaaa
     2521 ccgggcatca tgcgcagtcg cgggcggcag gtcacgcccg cgcccgaaat accctgccgc
     2581 aagccccatg aatccgccga agatctacga ccgtcccgaa gccgtctggg cgcacacgcc
     2641 ggaccgccag cccttcctgt acatggccga cggcctgctg tcgctgcact tcgaggccgc
     2701 cagcgtgcag agccgcatgg acccggccgc accggcgcgc ctcacgctcg gctacacgcg
     2761 cacgatgatg ggggcggcgc tgctgctgct cggcccggtc cccgggcgca tcgcgatgat
     2821 cgggctcggc ggcggctcgc tggtcaagta ctgccgccag cacctgcccg aagccgtcat
     2881 cgaggtcgcc gagatcagcg ccgaggtcat cgcgctgcgc gagcgcttct gcgtgccggc
     2941 cgattccgac cacctgcgcg tgctgtgcac cgacggcgcg gccttcgtcg cgacggccgg
     3001 cgccggcgag tgggatctgc tgctcgtcga cggcttcggc cccggcggcc agccgccgca
     3061 gctcggctcg cagcgcttct atgacgactg ccgcgcggca ctcggcgccg acggcctgct
     3121 ggtcgcgaac ctggccggca gcgaccccag gctcgatctg tacctgtcgc ggctggaaac
     3181 cgccttcggc cgcgtgctga acctgccggc cgccgactcc gacaaccgca tcgccatcgc
     3241 cgggcgcggg ctggccaccg ccgacggccg gctgtgcgca cggctgaagc ggctcgcgcc
     3301 gcagcacgac ctcgacctcg gccggacctg cgcagcgctg ctcgatgcac gccgcgcctg
     3361 ataccgatca cacgtaaacg catgattttc ccccctctcc ccaacccctc tcccgcgagg
     3421 ggcgaggggc tgaaaagcga agcgttccga tgcgatcggt atgagcatct ggccgcccgg
     3481 ccggcgcttc ggctatcctt cgcgccctct gcggagaggt ggccgagcgg tttaaggctc
     3541 tcgtcttgaa aacgagcgtc ggctaatccc cgaccgtggg ttcgaatccc accctctccg
     3601 cca
//
LOCUS       DAOQIE010000358         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16332_length_3595_cov_5.509605, whole genome shotgun sequence.
ACCESSION   DAOQIE010000358 DAOQIE010000000
VERSION     DAOQIE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16332_length_3595_cov_5.509605"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK144_16695"
     CDS             <1..665
                     /locus_tag="PK144_16695"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNF67411.1"
                     /translation="HRLGPETRAVLLQNGMGTADAVHLRFPRLMTVIGTTTNAAYRST
                     PRRLVRARRGETWIGDGPLPLPVIERHILSEALAESGLHAAWDNDLKLRLWHKLVLNC
                     AVNPVTALLDVPNGVLAGTGPARTLVAAVCIEAAAVMQAEGLPADARTLIEQVVRLAH
                     ESGRNVSSMRADVRAGRTTEIDFINGYLLARAQAHGLQAPCNRTLTELIRLRTATAGG
                     AA"
     gene            662..1363
                     /locus_tag="PK144_16700"
     CDS             662..1363
                     /locus_tag="PK144_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF67412.1"
                     /translation="MMSRPPLLLIPGLLCDGRLWQHQLAALADAADMTVGDHSTHDNL
                     YEIAATLLTKAPPRFAVAGLSMGGYIAFELLRQAPERITHLALINTAARPDSPEKRQR
                     RLDFMALATRGRFRGVSHALLPLLIHRDRLDDRELAGTVSAMARDSGVEAYLRESRAI
                     VDRPDSVPMLSGIACPTVVIGGLQDQLTPPECQQEIADGIPGAELILLDRCGHLAPLE
                     HPQAVNAALRKLLTR"
     gene            complement(1464..1913)
                     /locus_tag="PK144_16705"
     CDS             complement(1464..1913)
                     /locus_tag="PK144_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNF67413.1"
                     /translation="MAHVVFFEKPGCGGNARQKAALRAAGHSLEVRDLLTWPWTAAQL
                     QEWFSGLPVADWFNRSAPRVRDGEILPERLSAEAALTLMLAEPLLIRRPLLEAAGEKR
                     VGWDQEAVDAWLGLAANAATVGEGCPKGEKMAPCPAPVPGTAADTAQ"
     gene            complement(2136..>3595)
                     /locus_tag="PK144_16710"
     CDS             complement(2136..>3595)
                     /locus_tag="PK144_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF67414.1"
                     /translation="AVRARHFALPGERCTALDDTRRLFRAFVEDFLKKHGVDVREVVE
                     LRCQECQHVFAEADVRGRIENGKATIFCPDGHSTPIDAAFKDARANNPELTDCLAALK
                     TVVDRRIAQEAQTASDKVSSATISPTSAGVSVPTPGKSLNLLHLSDLHFTADTDPVQM
                     LQPLVADLTDQQGLKLEKLDYLVVSGDCTCIGSAAEFERVHEFLESLVKKFGLSAHRC
                     VLVPGNHDLSWKEKVYEWQDAGEVDTAKLPDGNFIRQGDGYLVRMDEAIYGQRFKNFA
                     TFHHQFMQQPYPLEVQKQAQVYIHADDKIQFIGLNSSCRIDRYNKGRASIDGRALSHA
                     LMEAEKQIKDAVTGGRIPKSADVLRIAVWHHPITGNEKIFDDAFVQLLQQADVRLCLH
                     GHIHENRADLIGPTHPTRKLHTIGAGSFGAVAKDRPESTPRLYNLIEIAADRSQVRVH
                     TRQMPKAGGAWKAYADWPDDQGIIRSYYDIKLRKG"
BASE COUNT          573 a         1183 c         1193 g          646 t
ORIGIN      
        1 cccaccgcct cggccccgaa acccgtgccg tgctgctgca aaacggcatg ggcacggccg
       61 atgccgtgca cctgcgcttt ccgcggctga tgaccgtgat cggcaccacg accaacgccg
      121 cctaccgcag caccccgcgg cgactggtgc gcgcacgtcg cggcgaaacc tggatcggtg
      181 acggcccgct gccgctgccg gtgatcgagc ggcacatcct cagcgaggcg ctggccgaat
      241 ccggcctgca cgccgcctgg gacaatgacc tgaagctgcg gctctggcac aagctcgtgc
      301 tgaactgcgc ggtcaacccg gtcacggcgc tgctcgacgt gccgaacggc gtgcttgccg
      361 ggaccggtcc ggcgcgcacg ctggtggcgg cggtctgcat cgaggctgcc gcggtcatgc
      421 aggccgaggg cctgccggcc gatgcgcgca cgctgatcga gcaggtcgtt cgcctcgcgc
      481 acgaaagcgg gcgcaatgtg tcctcgatgc gcgccgacgt gcgcgccggc cgcaccaccg
      541 aaatcgattt catcaacggt tatctgctcg cccgcgccca ggcccacggc ctgcaggcgc
      601 cgtgcaaccg cacgctgacc gagctgatcc gcctgcgcac cgccaccgcc ggaggtgcag
      661 catgatgtcc cgcccgccgc tcttgctgat tcccggcctg ctctgcgacg gccggctgtg
      721 gcagcaccag ctcgccgcgc tcgccgatgc cgccgacatg acggtcggcg atcactcgac
      781 gcacgacaac ctctacgaga tcgccgccac gctgctgacc aaggcgccgc cgcgtttcgc
      841 ggtcgccggg ctgtcgatgg gcggttacat cgccttcgaa ctgctgcgcc aggcgccgga
      901 gcgcatcacg catctggcgc tgatcaacac cgccgcgcgc ccggactcgc ccgaaaagcg
      961 ccagcggcgg ctggatttca tggcgctcgc cacgcgcgga cgtttccgcg gcgtcagtca
     1021 cgcactgctg ccgctcttga tccaccgcga ccggctcgat gaccgcgagc ttgccggcac
     1081 ggtcagcgcg atggcgcgcg attcgggcgt ggaagcctat ctgcgcgaaa gccgcgccat
     1141 cgtcgatcgc cccgacagcg tgccgatgct ctccggcatc gcctgcccga ccgtcgtcat
     1201 cggcggcctg caggatcagc tgacgccgcc cgagtgccag caggaaatcg ccgacggcat
     1261 tcccggggcc gagctgatcc tgctcgatcg ctgcggccat ctggcgccgc tcgaacaccc
     1321 gcaggcggtc aacgccgcgc tgcgcaagct gctgacacgc tgacgccgcg cccccgcccg
     1381 cctgtcgttc aagcccctcg cccctcgcgg cagagggctt gggtggaggg gggaaaaaac
     1441 catgcatttc cgggtgatcg atatcactgc gccgtatcgg ccgccgtgcc cggcaccggc
     1501 gccgggcacg gcgccatctt ctcgcccttc gggcagcctt cgccgaccgt cgcggcattg
     1561 gcggcgaggc cgagccaggc atcgaccgcc tcctgatccc agccgacgcg cttctcgcct
     1621 gcagcttcga gcagcgggcg gcggatcagc agcggttcgg cgagcatcag tgtcagtgcg
     1681 gcttcggcgc tcagccgctc gggcaggatt tcgccgtcgc gcaccctggg cgccgagcgg
     1741 ttgaaccagt cggcgaccgg caggccgctg aaccattcct gcagctgggc ggccgtccac
     1801 ggccaggtga gcaggtcgcg cacttcgagg ctgtggccgg cggcgcgcag ggcggctttc
     1861 tgcctggcgt tgccgccgca gccgggtttt tcaaagaaga cgacgtgggc catcagccgg
     1921 actctccgtg caggcgttcg caggatcacc ttgtcgcaaa cacgacaaac cggccgcgga
     1981 atccgggccg tggcgggggt ggagcaggat gcgtgccggc gatggcgccc gaggcccgcg
     2041 gcgatcgggc gcaggggcag cggtgccgga tcggtcggtg cccttgtccg ggtgagtgag
     2101 tcgcgaaggg cggacgggag ggggtgcggg cgggatcagc ccttgcgcag cttgatgtcg
     2161 taataactcc ggatgatgcc ctggtcgtcc ggccagtccg catacgcttt ccaggcacca
     2221 ccggctttcg gcatctggcg ggtgtggaca cgtacctgac tgcgatcggc ggcaatttcg
     2281 atcaggttgt agaggcgcgg ggtagattcg ggacggtcct tggcgaccgc gccgaaggaa
     2341 ccggcgccga tggtgtgcag cttgcgggtc ggatgggtgg gtccgatcag gtcggcgcgg
     2401 ttttcgtgga tgtggccgtg caggcacagc cgcacgtcag cctgctgcag aagctggacg
     2461 aaggcgtcgt cgaagatttt ttcgttgccg gtgatcggat gatgccagac ggcgatgcgc
     2521 agcacgtcgg cggatttcgg aatgcgaccg ccggttaccg catccttgat ttgcttctcc
     2581 gcctccatga gcgcatgcga cagggcgcgg ccgtcgatac tggctcgccc cttgttgtat
     2641 cggtcgattc gacaggagga attgaggcca atgaactgga ttttgtcgtc ggcgtggatg
     2701 tagacctgtg cctgcttttg cacttcgagc ggatagggct gctgcatgaa ctggtgatga
     2761 aaggttgcga agttcttgaa gcgctggccg taaatcgctt cgtccatacg cacgagatag
     2821 ccgtcgcctt gccggatgaa gttgccatcc gggagctttg ccgtatcgac ttctccagcg
     2881 tcctgccatt catagacttt ttctttccaa ctgaggtcgt gatttccagg caccagtacg
     2941 cagcggtgtg cggacagacc aaatttcttg accagtgact ccagaaactc atgcactcgc
     3001 tcgaattcgg cagcactgcc aatacaagtg cagtcgccgg agacgacgag gtaatcgagt
     3061 ttctccagct tcaaaccctg ctggtcggtg aggtcggcga ccagcggttg cagcatctgc
     3121 accgggtcgg tatcagcggt gaaatgcagg tcactgaggt gcagcagatt tagagacttt
     3181 ccgggggtgg gcacgctgac tccggctgac gtggggctga tcgtcgctga ggaaacttta
     3241 tcactcgcgg tctgggcttc ctgcgcaatt cggcggtcca cgacggtttt caaggcggcg
     3301 aggcagtcgg tcagttccgg attgtttgcg cgtgcatcct tgaatgcagc gtcgattggt
     3361 gtgctgtggc catccggaca gaagatcgta gcttttccgt tttcgatgcg cccacgtaca
     3421 tccgcctcgg caaacacatg ctggcattcc tgacagcgca gttcgaccac ttcgcgcaca
     3481 tcgacgccgt gctttttcag aaagtcctcg acgaaagcac ggaacagccg acgggtatcg
     3541 tccagggctg tgcaacgctc ccctggtagt gcaaagtgtc gcgccctcac agccg
//
LOCUS       DAOQIE010000359         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16537_length_3565_cov_7.398291, whole genome shotgun sequence.
ACCESSION   DAOQIE010000359 DAOQIE010000000
VERSION     DAOQIE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16537_length_3565_cov_7.398291"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..759
                     /gene="ureE"
                     /locus_tag="PK144_16715"
     CDS             256..759
                     /gene="ureE"
                     /locus_tag="PK144_16715"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HNF67415.1"
                     /translation="MTLALTEVLADDGPHDATLTLPFDARQKSRLLTRLDSGEPVGLF
                     LPRGTVLRGGDRLRAADGRVVRVRAAREQVSTVGTPGSLLLVRAAYHLGNRHVPLQIG
                     IGWLRYQRDHVLDALVESLGLEVGHEEVPFEPESGAYGGGHHHHGTAGAATDDAIAAP
                     LSLIAAV"
     gene            765..1490
                     /locus_tag="PK144_16720"
     CDS             765..1490
                     /locus_tag="PK144_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HNF67416.1"
                     /translation="MSGAPAPLVPPRPSAGGAGGLPLLRLLQLASPQLPVGAFAWSSG
                     LEGAVEAGWVQDDAGLGDWLAGLLGGPWAHQDLPALARLHAAWTLGDAVAVAHWNDWL
                     SATRETAELRAEDRALGRALARLLATLDLPEAAAWAGAEAHTGYATMFALAAARWGVP
                     VEATLQALAWAWIENQVVAATRLMPLGQSAAQRRLAALMPDIEAAVALALALEDDELG
                     CSAAGLALASCRHETQYTRLFRS"
     gene            1583..2209
                     /gene="ureG"
                     /locus_tag="PK144_16725"
     CDS             1583..2209
                     /gene="ureG"
                     /locus_tag="PK144_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HNF67417.1"
                     /translation="MSTKPTLRVGIGGPVGSGKTALTEALCKRMRSRYNMAVVTNDIY
                     TYEDAQFLTRAGALDPERILGVETGGCPHTAIREDASANLAAVDQLCTRFPGLDLVLV
                     ESGGDNLSATFSPELSDLTLYVIDVAAGDKIPRKGGPGITRSDLLIINKIDLAPHVGA
                     SLEVMDRDAKKMRGERPFVFTNMKTGEGLEAVIAFIEEKGMLDVALPA"
     gene            complement(2458..2721)
                     /locus_tag="PK144_16730"
     CDS             complement(2458..2721)
                     /locus_tag="PK144_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF67418.1"
                     /translation="MTHIIAEPCIGCKDTACVDVCPVDCIYEHRNDSAAQSFIQPEEC
                     IDCGLCVDACPVEAIFPIEEVPDKWHRFIAMNYEQFRLEAPGI"
     gene            complement(2767..3051)
                     /locus_tag="PK144_16735"
     CDS             complement(2767..3051)
                     /locus_tag="PK144_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67419.1"
                     /translation="MASEDAIEVVAKFGEEKDGSAWATLEYYMENNPRQTEWRRIPGV
                     VTGADKDDAMAKAEAIAVELSDLEIQQLQAAVRRLYEKGRMIKRLEFPTT"
BASE COUNT          534 a         1189 c         1195 g          647 t
ORIGIN      
        1 ttgagcatcg cggaagcgat tgccgctggt gaaaccggat ttgctgcggc gcaccaattg
       61 gcagtgctat ggtccaagta ccgtgagccc gtgccatcgc gccgctcgcg gtccagggag
      121 ggccgctgtg cgatgtgcgt gttcgcgatc ctgctgtgct gccgttcccc cggcggttgc
      181 gcggccttcc ccccgtcccc cccgcgggta tccgcatgcg tgcgtacccg aagccttgtt
      241 tcaggaggct gccccatgac cctcgccctg accgaagtgc tcgccgatga cggcccgcac
      301 gacgcgacgc tgaccctgcc cttcgatgcc cggcagaaat cccggctgct gacccgcctc
      361 gacagcggcg agccggtcgg cctgttcctg ccgcgcggca ccgtgctgcg cggcggtgac
      421 cgcctgcgtg ctgccgacgg tcgcgtcgtg cgggtgcgtg cggcgcgcga gcaggtttcg
      481 acggtcggga cgcccggcag cctgctgctg gtgcgcgccg cctaccacct cggcaaccgt
      541 cacgtgccgc tgcagatcgg catcggctgg ctgcgctacc agcgcgacca cgtgctcgat
      601 gcgctggtcg agagcctcgg cctcgaggtc ggtcacgagg aggtgccgtt cgagcccgaa
      661 agcggtgcct acggcggcgg ccatcaccac cacggcaccg ccggggccgc tacggacgac
      721 gccattgccg caccgctgtc gctgatcgcg gcggtgtgac ggccgtgtcc ggcgcccctg
      781 cgccgctcgt gccgccccgc ccgtccgccg gcggggcggg cggcctgccg ctgctgcgcc
      841 tgctgcagct cgcgagcccg caattgccgg tcggggcgtt cgcgtggtcg agcgggctgg
      901 aaggtgcggt cgaggccggc tgggttcagg acgatgccgg gctcggtgac tggctcgccg
      961 gtctgctcgg cggcccctgg gcacatcagg acctgccggc gcttgcgcgc ctgcacgcgg
     1021 cctggacgct tggtgatgcg gtcgcggtcg cgcactggaa cgactggctg tccgcgaccc
     1081 gcgaaaccgc cgaactgcgc gccgaggacc gcgcgctcgg ccgcgcgctg gcgcggctgc
     1141 tggccacgct cgacctgccc gaggccgccg cctgggccgg tgccgaggcg cacaccggct
     1201 acgccacgat gttcgccctc gccgccgccc gctggggcgt gccggtcgag gcgacgctgc
     1261 aggcgctcgc ctgggcctgg atcgagaatc aggtggtggc cgcgacccgg ctgatgccgc
     1321 tcggccagag cgccgcccag cgccggctgg cggcgctgat gcccgacatc gaggcggccg
     1381 tcgcgcttgc gctcgcgctc gaagacgacg aactcggctg ctcggccgcc ggcctcgcgc
     1441 tcgcgagctg ccggcacgaa acccagtaca cgcggctgtt ccgctcctga gcctgctttc
     1501 cggcggtggc gctcacgggt cgcaacggcc cggctaagct gccccgcatc cttccccgtt
     1561 cccctgcgag gactcccgcc tcatgagcac gaaacccacc ctgcgcgtcg gcatcggcgg
     1621 tccggtcggc tccggcaaga cggcgctgac cgaggcgctc tgcaagcgca tgcgcagccg
     1681 ctacaacatg gccgtggtca ccaacgacat ctacacgtac gaggatgcgc agttcctgac
     1741 ccgtgccggc gcgctcgacc ccgagcgcat cctcggcgtc gagaccggcg gctgcccgca
     1801 cacggcgatc cgcgaggacg cctcggccaa cctcgccgcg gtcgatcagc tctgcacgcg
     1861 cttccccgga ctcgatctgg tgctggtgga atcgggcggt gacaacctct cggccacctt
     1921 cagccccgag ctttccgacc tgacgctgta cgtgatcgat gtcgccgccg gcgacaagat
     1981 cccgcgcaag ggcggtccgg gcatcacgcg ctcggatctg ctgatcatca acaagatcga
     2041 cctcgccccg cacgtcggcg cctcgctgga agtcatggac cgcgatgcga agaagatgcg
     2101 cggcgagcgg ccgttcgtgt tcacgaacat gaagaccggc gaggggctgg aggccgtgat
     2161 cgcgttcatc gaggagaagg gaatgctgga tgtggcgttg ccggcctgag ggcgcctgca
     2221 ttcagccggc ctgacggctg cagcccggct tcctttgagc agaagtcggg caggggacgc
     2281 attcatgcgc atgggtgacg gcaatcggca atggcgtggt acgcatccat gagcgcaccg
     2341 cagccggtcg atgccgatca caggtcaggg catgattttt attgccttcc ctctttccag
     2401 cccctctccc gcaaggggcg aggggctgaa aaacgaagcg ttccgatgtg atcggtatca
     2461 gatgcccggt gcttccagcc tgaattgttc gtagttcatg gcaatgaacc ggtgccattt
     2521 gtccggcact tcctctattg gaaagatggc ttcgaccgga caggcatcga cgcaaaggcc
     2581 acagtcgatg cattcctccg gctggatgaa agactgtgcg gccgagtcgt tgcgatgctc
     2641 gtaaatgcag tccaccgggc agacgtccac gcaggcggtg tccttgcagc cgatgcaggg
     2701 ttcggcaatg atgtgcgtca tgttcggact ccttgaaatt ccttgcaaaa atcagaggat
     2761 ggcggatcag gtcgtcggga attcgagtcg cttgatcatc cttccttttt cataaagcct
     2821 gcgcacggcc gcctgcagtt gctggatttc gagatcgctg agttcaacgg caatcgcctc
     2881 ggctttcgcc atggcatcgt ccttgtctgc acccgtgacg accccgggga tgcggcgcca
     2941 ttcggtctgg cgcggattgt tttccatgta gtattcgaga gtcgcccacg cgctgccgtc
     3001 tttttcttcg ccgaatttgg cgaccacttc aattgcatct tcactggcca tgtctttgcc
     3061 tccctgaagg tgtgggattt gttgcggttg cggttgcggt tgcagacggg catgacctgc
     3121 gggtgcttgc cgcaatcgct gcaatgccgc ggtcagcccc cgcatgcatg tggaatgcgg
     3181 ggttgaggct agcagcaggt catcggatgg ccaagcaatc gcgggcagaa accgcagtcg
     3241 ggtgagggaa agttaatgtt ggcggagttc gcgtgaaagc ctccggcaag caggggtttc
     3301 ggggtgcatc cgtcaagcat tgctggtgat ccgtaaccgg aagccggatt tcatgcgggt
     3361 ttcattcgaa tttcatttga aatggcgatg ccattcatgt ggcatggccg gacaggaatg
     3421 cgtggcgtct ggcgaacgac gggtaagcaa tctgtcgtgg cggtgcgttc acgacgacag
     3481 gggcatgcgg cctggccggc agtacccgaa tggcactgcg gatgccgcat cccgctcact
     3541 catcccgata ccgccccgcg atccc
//
LOCUS       DAOQIE010000360         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16572_length_3560_cov_7.287874, whole genome shotgun sequence.
ACCESSION   DAOQIE010000360 DAOQIE010000000
VERSION     DAOQIE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_3560_cov_7.287874"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="pyrE"
                     /locus_tag="PK144_16740"
     CDS             complement(<1..282)
                     /gene="pyrE"
                     /locus_tag="PK144_16740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254018.1"
                     /note="involved in fifth step of pyrimidine biosynthesis;
                     converts orotidine 5'-phosphate and diphosphate to orotate
                     and 5-phospho-alpha-D-ribose 1-diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNF67420.1"
                     /translation="MQPYQRDFLDFAVSRGVLRFGEFTLKSGRISPYFFNAGLFNTGG
                     ALARLGRAYAQAITDAGIDYDLLFGPAYKGIPLAAATSVQLIEQYGRDVP"
     gene            753..1454
                     /locus_tag="PK144_16745"
     CDS             753..1454
                     /locus_tag="PK144_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67421.1"
                     /translation="MRHLTRSTLAAGALKLGGLAAFQAEASVVYVDLVPDLVTANNAT
                     IGNFLDLNGDGSDDFAFGHDATDTGSDGQTDAIAVVFSDPSFVMIANDAGFPSATRFG
                     TGSTIDAAAFADANKVGVLAIDDFDLNGGGVRAQAGNWYNGGAGTIGYVGFAMTGLPG
                     DVVGTDYHFGWMRVGVSAYDNTSNSLVITAYDYAYESVAGVGITIPEPAGWALFAAGG
                     TALAARRRRRTATTA"
     gene            1988..2872
                     /locus_tag="PK144_16750"
     CDS             1988..2872
                     /locus_tag="PK144_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67422.1"
                     /translation="MRVLAVGPQRLAPVRALTRLPLRVQTGADCWLAFLPEAGDCPAG
                     AAALHPDGALWIAVVPALRRRGVGRALHATAMAAAQAQGLTRVVSWRGTDTPEAAAFC
                     AAVGLQVVRTIETFETTLANTVRFFGRYRDRLLARGRLPAGILEQNDGEVPWGAYGAA
                     LIEAFGPPAAVRLDRILRSGLEPNEWAQALHLDGRPVAVTLGSRGSDWASTDLYLVQP
                     AFRRGWAHIESKFRAARRLHRRHREIERHRFSTADEHGDTRRAYEVGIRRGFEAARIA
                     CEHYYACELEKPSCRAAC"
     gene            2854..>3560
                     /locus_tag="PK144_16755"
     CDS             2854..>3560
                     /locus_tag="PK144_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF67423.1"
                     /translation="MSRCLLIALEGWDEHIAAPLLARGLLPNLARLRARGRHGSLTPI
                     LADCPPACWASLNTGRWPDVHDVLAAVETDPAHGGARAVDSRSLQRAPLWEHAAAAGL
                     ASLRIGLPASSPARGAGGCVGDAYPGRCAADGGEWLALADGALGGFDAGTCAQLDALR
                     VDPAELDAAVLAPLMPEIGTIDPHGDRRPALAALALARSFSLHAAATWLLETRPGPLT
                     LVCYPLLAELSAIFAAYL"
BASE COUNT          522 a         1262 c         1228 g          548 t
ORIGIN      
        1 cggaacgtca cggccgtact gttcgatgag ctgcaccgag gtcgccgcgg ccagcgggat
       61 gcccttgtag gccgggccga acaacaggtc gtagtcgatg ccggcatcgg tgatcgcctg
      121 cgcgtaggcc cggccgagcc gggccagcgc gccaccggtg ttgaacaggc cggcattgaa
      181 gaagtacggg ctgatgcggc cggatttcag ggtgaattcg ccgaagcgca gcacgccgcg
      241 gctgacggcg aaatcgagga agtcgcgctg atagggctgc atggggggcg gattccggcg
      301 gaaacgggaa ggaggaccga ttatgccggt gcgatgtgtc cccgagggtt cgttcctgcc
      361 gtgccggcag ccgtggctct tgtgacagcg cggcttacct ggagacccgt cgtccggtcc
      421 gggccgcggc cgtacgggct tcggcaaaag cacggaaggc aggcgcagtc gaggagcatg
      481 caaatcaggc atgtacgaaa gcggttggag tcagcgggcg gtggcggcgg gcacgagcaa
      541 aagttcccgc atggattgca aagaaaagtt tctgaaagcc gggcttgaca aagtttcacc
      601 gaggatgacg ccgcacgtcg caccactgtc cggaccttgc aggctgcctg tcgctggcag
      661 ccgtccggcg gtgtgggtgt gatcgatgca gaaccgcggc cattgaaccc ggacgtcacc
      721 tttccagcct tttcgcccaa ggatccccgc acatgcgcca tctgacccgc tccaccctcg
      781 ccgccggcgc gctgaagctc ggcggcctcg ccgccttcca ggccgaagcc tcggtcgtct
      841 acgtcgatct cgttcccgac ctcgtcaccg ccaacaatgc gaccatcggt aattttctcg
      901 acctcaacgg cgacggttcg gatgatttcg ccttcgggca tgacgcaaca gataccggca
      961 gcgatggcca gaccgacgcg atcgcagttg tattcagtga tccgtcgttc gtcatgatcg
     1021 ccaacgatgc cggctttccg agcgcaacgc gtttcggtac cggcagcacc atcgacgccg
     1081 ccgccttcgc cgatgccaac aaggtcggcg tcctggccat cgatgatttc gatctgaacg
     1141 gtggcggcgt gcgcgcgcag gccggcaact ggtacaacgg cggcgccggc acgatcggct
     1201 acgtcggatt cgcgatgacc ggcctgcccg gtgacgtggt cggtaccgac tatcacttcg
     1261 gctggatgcg cgtcggcgtc agcgcctacg acaacaccag caactcgctg gtgatcaccg
     1321 cttacgacta cgcttacgag tcggtcgccg gggtcggcat cacgatcccc gagccggcgg
     1381 gctgggcgct gttcgccgcc ggtggcacgg cgctcgccgc gcgccggcgt cgccggacgg
     1441 ccacgaccgc ctgatcgact ccggctgcct gccggcaagc agggcttgcc ggccgggtct
     1501 ggcagatcgc tgcggctgcg tccggcgacc cggtcgcacg tctgcccgcc cttccgggaa
     1561 accggattca cttcggatca acgccctgac tgcgacgccg actccggcgg gccgtagtcg
     1621 tccggctttg gcaatcgtgc atgcggccga aaaaaaaatc ggcatgcaag ccaaacttga
     1681 caattattcg gggaagatta acacccgttg cagtgcatca accgccaccg gctcctgagc
     1741 accgtgctgt caggtgtgcc ggtcagaagc ccggatcgag ccacggactc ccccggcaca
     1801 cgcgccttca ttccgcccaa cagcacaagg acgcccccgc catgtccctc ctgcccgttg
     1861 atccgcaagg cggcctgccg caatccggtc cgtcctgcgc actgctccgt cccggcccgg
     1921 acagggccgg gtgtgccgtc cgcgtgcaca tgtcctgcct gccctgcggg cgggcgtcgt
     1981 tcctctgatg cgggtcctgg ccgttgggcc gcagcgcctc gcgccggtac gggcgctgac
     2041 gcgtctgccg ctgcgcgtgc agaccggcgc cgactgctgg ctggccttcc tgcccgaggc
     2101 cggcgactgc ccggccgggg ctgccgcgct gcatccggat ggcgcgctgt ggatcgcggt
     2161 cgtgccggcc ctgcgccggc gcggcgtcgg ccgggcgctg catgcgacgg ccatggcggc
     2221 agcgcaggcg caggggctga cgcgcgtggt gagctggcgc gggacggaca caccggaagc
     2281 cgcggccttc tgcgcagccg tcggcctgca ggtggtgcgt accatcgaaa ccttcgaaac
     2341 cacgctcgcg aacaccgtgc gcttcttcgg ccgctaccgc gaccgcctgc tggcgcgcgg
     2401 gcggctgccg gccggcatcc tggagcagaa cgatggcgag gtgccctggg gcgcctatgg
     2461 cgcggcgctg atcgaggcct tcggaccgcc ggcggcggtg cggctcgatc gcatcctgcg
     2521 cagcggcctg gagccgaacg aatgggcgca ggcgctgcac ctcgacggcc ggccggtcgc
     2581 ggtgacgctc ggcagccgcg gcagcgactg ggcctcgacc gacctgtatc tggtgcagcc
     2641 ggccttccgc cgcggctggg cgcacatcga aagcaagttc cgcgcggccc ggcgcctgca
     2701 ccggcgccac cgcgagatcg aacgccaccg cttcagcacc gccgacgaac acggcgacac
     2761 gcgccgcgcc tacgaagtcg gcatccgccg cggattcgag gctgcgcgca tcgcctgcga
     2821 acactattac gcctgcgaac tggagaagcc ctcgtgtcgc gctgcctgct gatcgccctc
     2881 gaaggctggg acgaacacat cgccgcaccg ctgctggcgc gcgggctgct gccgaacctc
     2941 gcccggctgc gcgcacgcgg tcgtcatggc tcgctgacgc cgatcctcgc cgactgcccg
     3001 ccggcctgct gggcgagcct gaacaccggc cgctggccgg acgtgcatga cgtgctggcc
     3061 gccgtcgaga ccgatccggc ccacggcggc gcgcgcgcgg tcgacagccg cagcctgcag
     3121 cgtgcgccgc tgtgggagca cgcggccgcc gccggcctgg ccagcctgcg catcggcctg
     3181 ccggcgagca gtccggcacg cggtgccggc ggctgcgtcg gcgatgccta tccggggcgc
     3241 tgtgcggccg atggcggcga gtggctggcg ctcgccgacg gcgcactcgg cggcttcgac
     3301 gccggaacct gcgcgcagct cgatgcgctg cgcgtcgatc cggccgaact cgacgccgcg
     3361 gtgctcgcgc cgctgatgcc ggagatcggc acgatcgacc cgcatggcga tcgccggccg
     3421 gcgctggcgg cgctggcact ggcgcgcagc ttcagcctgc atgccgcggc gacctggctg
     3481 ctggaaaccc gccccgggcc gctgacgctc gtctgctatc cgctgcttgc cgaactgtcg
     3541 gcgatctttg ccgcctacct
//
LOCUS       DAOQIE010000361         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16596_length_3558_cov_5.690836, whole genome shotgun sequence.
ACCESSION   DAOQIE010000361 DAOQIE010000000
VERSION     DAOQIE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16596_length_3558_cov_5.690836"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PK144_16760"
     CDS             <1..1557
                     /locus_tag="PK144_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF67424.1"
                     /translation="VELFRPDVVLAPYLKRAIPEAVWRHVPCLIVHPGIPGDRGPAAL
                     DRAILERRSHWGVTVLQAVAEFDAGPIWAHRSFPMREAPKASLYRREVTTAAVEAVLE
                     ALSRLAAGWQPEAQHEGLPGRTHRPVSLAERTIDWTSDRTADVLRKIHSADGYPGVRG
                     ELAGVTCRLYDAHAEHGLRGAPGALLARRGEALCRATVDGAVWIGHVRREDEHALKLP
                     ATLALAAELAGLSDLPELPEDDDGRVVYREADGVGWIDFPFHNGAMGTPHCRRLAETV
                     RMARSRGVRVLVLTGGPEFWSNGIHLNLIEAADSPADASMHNIEAMNDLVAELLECRG
                     LWLIAALRGNAGAGGCFLALAADEVLAHDGVVLNPHYRNMGNLYGSEYWTYLLPKRVG
                     EDGVAHVLDRRLPLGAHEAAAIGLIDGCIGPAAGFMEAVDAHARAVAADPDFPRLIAA
                     KLDGYAADEREKPLAVYRAEELERMRLNFYGFDPSYHVARHAFVHRRPQSWTPLHLAI
                     HRRRTPPQPT"
     gene            1554..3056
                     /locus_tag="PK144_16765"
     CDS             1554..3056
                     /locus_tag="PK144_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF67425.1"
                     /translation="MSAAEDEHLPGAPAGSSGAAAPLPVVLVVDDEPRSLQTITRVLE
                     DEFDVLTATGSAEAERILAREWVQVILCDQRMPGETGVVFLARVREQWPDTVRIIVSG
                     YTDAEDIIAGVNRAGIYQYLTKPWTPDALLLTVRGAVQLARLQRENETLALELKLTAP
                     AVQQRLGARRRQLREQHAFARLVRAPGSPLDEVCALAARIALYDIPLLIAGESGTGKE
                     LLARAVHYNSPRAERPFVVENCAALPDTLLESELFGHRKGAYTGAFEDRQGLFAQADG
                     GTLFLDEIGEISPAFQVRLLRVLQEGEFRPVGSARTQRCDVRVIAATNRDLAAEVRAG
                     RFREDLYYRLAGFVLEVPPLRARPDDIAPIAQALIADIAAAFGVRIDGCAPETLARLV
                     RHDWPGNVRELRNELQRLIALADEPLLGPQRLSPGIGPGAAEAASGDDPAVAAGGTLV
                     ARLDRLERAILEETLNRLRWNKTRAADELGLSRVGLRAKLARHGLGPADD"
     gene            3069..>3558
                     /locus_tag="PK144_16770"
     CDS             3069..>3558
                     /locus_tag="PK144_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNF67426.1"
                     /translation="MAGGYDTDDTAGLPDPATLPGGEAADAAWIEVIRKMDEVYADLV
                     RYQVELEHKHAELEDAHGFISGVLAAMSDALIVCDAEGRIERVNPALERLTGRHEAEL
                     HAQPFASLCLPAAQPLAGALLREAEADAPVERELLLAGPGGTAVPLAFHATVRRDATG
                     HRL"
BASE COUNT          476 a         1347 c         1274 g          461 t
ORIGIN      
        1 gtcgagctgt tccggcccga tgtcgtgctc gcgccctacc tcaagcgcgc gatccccgag
       61 gcggtctggc gccatgtgcc ctgcctgatc gtgcatccgg gcatccccgg ggaccgcggg
      121 ccggcggcgc tcgatcgcgc gatcctcgaa cgccgttcgc actggggcgt gaccgtgctg
      181 caggcggtcg ccgagttcga tgccggcccg atctgggcgc atcgcagctt cccgatgcgc
      241 gaggcgccga aggcgagcct gtaccggcgc gaggtcacga ccgcggcggt cgaggccgtg
      301 ctcgaagccc tgtcgcgcct cgccgccggc tggcagcccg aagcgcagca cgagggactt
      361 cccgggcgca cgcaccggcc ggtgtcgctc gccgagcgca cgatcgactg gacgagcgac
      421 cgcaccgccg acgtgctgcg caagatccac tccgccgatg gctaccccgg cgtgcgcggc
      481 gagctggccg gcgtcacctg ccggctgtac gacgcccacg ccgaacacgg gttgcgcggt
      541 gcccccggcg cgctgctggc ccgccgcggc gaggcgctct gccgggcgac cgtcgacggc
      601 gcggtctgga tcggccacgt gcgccgcgag gacgagcacg cgctgaagct gccggcgacg
      661 ctggcgctgg ccgcggaact cgcgggcctg agcgatctgc ccgaactgcc cgaggacgac
      721 gacggccggg tcgtctaccg cgaggccgac ggcgtcggct ggatcgactt cccgttccac
      781 aacggcgcga tgggcacgcc gcactgccgg cggctggccg aaaccgtgcg catggcgcgc
      841 agccgcggcg tgcgcgtgct ggtgctgacc ggcggcccgg agttctggtc gaacggcatc
      901 cacctgaacc tgatcgaggc cgccgacagc ccggccgatg cctcgatgca caacatcgag
      961 gccatgaacg acctcgtcgc cgagctgctc gaatgccgcg gcctgtggct gatcgcggcg
     1021 ctgcgcggca acgccggcgc cggcggctgc ttcctcgcgc tcgccgccga tgaagtcctc
     1081 gcgcacgacg gcgtggtgct gaacccgcac taccgcaaca tgggcaacct gtacggctcc
     1141 gagtactgga cctacctgct gcccaagcgc gtcggcgagg acggcgtcgc gcacgtgctc
     1201 gaccgccgcc tgccgctcgg tgcgcacgag gccgcggcaa tcggcctgat cgacggctgc
     1261 atcggcccgg ccgccggctt catggaggcc gtcgatgcgc atgcgcgcgc cgtcgctgcc
     1321 gatccggact tcccgcgcct gatcgccgcc aagctcgacg gctacgctgc cgacgagcgc
     1381 gagaagccgc tcgcggtcta tcgcgccgag gagctcgaac ggatgcggct gaacttctac
     1441 ggcttcgacc cgagctacca cgtcgcgcgc cacgccttcg tgcaccgccg gccgcagtcg
     1501 tggacgccgc tgcacctggc catccaccgc cggcgcacgc cgccgcagcc gacatgagcg
     1561 ccgccgagga cgagcacctg cccggtgccc cggccggcag cagcggtgcg gccgcaccgc
     1621 tgccggtggt gctggtcgtc gatgacgagc cgcgttcgct gcagacgatc acgcgcgtgc
     1681 tcgaagacga gttcgacgtg ctgaccgcca ccggcagcgc cgaggccgaa cgcatcctcg
     1741 cgcgcgaatg ggtgcaggtg atcctctgcg accagcgcat gccgggcgag accggggtgg
     1801 tgtttctggc gcgcgtgcgc gagcagtggc cggacacggt gcgcatcatc gtctccggct
     1861 acaccgacgc cgaggacatc atcgccggcg tcaaccgcgc cggcatctac cagtacctga
     1921 ccaagccgtg gacgccggat gcgctgctgc tgaccgtgcg cggcgccgtg cagctcgcgc
     1981 gcctgcagcg cgagaacgag acgctggcgc tggagctgaa gctgaccgcg ccggccgtgc
     2041 agcagcgcct cggcgcgcgc cgccggcagc tgcgcgagca gcacgccttc gcgcgcctcg
     2101 tgcgcgcgcc gggcagtccg ctcgatgagg tctgcgcgct ggccgcgcgc atcgcgctct
     2161 acgacatccc gctgctgatc gccggcgaat ccggcaccgg caaggagctg ctcgcgcgcg
     2221 ccgtgcacta caacagcccg cgcgccgaac gcccgttcgt ggtcgagaac tgcgcggccc
     2281 tgcccgacac gctgctcgaa agcgagctgt tcggccaccg caagggcgcc tacaccggcg
     2341 ccttcgagga ccgtcagggc ctgttcgcgc aggccgacgg cggcacgctg ttcctcgacg
     2401 agatcggcga gatctcgccg gccttccagg tgcggctgct gcgcgtgctg caggaaggcg
     2461 aattccgccc ggtcggcagc gcacgcacgc agcgctgcga cgtgcgcgtg atcgccgcga
     2521 ccaaccgcga tctggccgcc gaggtgcgcg ccgggcgctt ccgcgaggac ctgtactacc
     2581 gcctcgccgg cttcgtgctc gaagtgccgc cgctgcgcgc gcgccccgac gacatcgcgc
     2641 cgatcgcgca ggcgctgatc gccgacatcg ccgcggcctt cggcgtgcgc atcgacggct
     2701 gcgcaccgga aaccctcgcg cgcctcgtgc gccacgactg gcccggcaac gtccgcgagc
     2761 tgcgcaacga gctgcagcgc ctgatcgccc tcgccgatga gccgctgctc ggtccgcagc
     2821 ggctgtcgcc gggcatcggt cccggcgccg ccgaggccgc ctccggcgac gatcccgccg
     2881 tggccgccgg cggcacgctg gtcgcgcggc tcgaccggct ggagcgcgcg atccttgagg
     2941 aaaccctgaa ccggctgcgc tggaacaaga cgcgcgcggc cgacgaactc gggctgtcac
     3001 gggtcggcct gcgcgccaag ctcgcgcgcc acggtctcgg accggcggac gactgaggcg
     3061 tggcggcgat ggcgggcggc tacgacaccg acgacacggc cggcctgccc gatccggcga
     3121 cgctgccggg cggcgaggcc gccgatgcgg cgtggatcga ggtgatccgc aagatggacg
     3181 aggtctacgc cgatctcgtg cgctatcagg tcgagctcga acacaagcac gccgaactgg
     3241 aggacgcgca cggcttcatc tccggcgtgc tggcggcgat gtcggatgcg ctgatcgtct
     3301 gcgatgccga gggtcgcatc gagcgcgtga acccggcgct ggagcgcctg accggccggc
     3361 atgaggccga gctgcacgcg cagccgttcg cgtcgctgtg cctgccggcc gcgcagccgc
     3421 tcgccggcgc gctgctgcgc gaggccgagg ccgacgcgcc ggtcgagcgc gaactgctgc
     3481 tcgccggccc cggcggcacg gccgtgccgc tcgccttcca cgccaccgtg cgccgcgatg
     3541 cgaccggcca ccggctcg
//
LOCUS       DAOQIE010000362         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16765_length_3537_cov_5.532165, whole genome shotgun sequence.
ACCESSION   DAOQIE010000362 DAOQIE010000000
VERSION     DAOQIE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16765_length_3537_cov_5.532165"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PK144_16775"
     CDS             complement(<1..688)
                     /locus_tag="PK144_16775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF67427.1"
                     /translation="MNRTDAPLAGALLLLASEATFAAMNALIKLASVNVPTATILAIR
                     HWLALALLLPIAWQRGWIGRQTPAKLRFHAARSLTGLAAMWTSFWAVGHLPLADAAVL
                     KMTSPLFVPLIAALWLGERLGPRIAWAALIGFAGVLAILKPGSGLFVPAALVGLASGA
                     LAALARVTIRRMSIGIGAGEIVFWFTLVGTLLTTPAALAAGVVPGVRDSGIIAALALL
                     GTLGQLLMTRA"
     gene            858..1956
                     /gene="prfB"
                     /locus_tag="PK144_16780"
     CDS             join(858..932,934..1956)
                     /gene="prfB"
                     /locus_tag="PK144_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823314.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNF67428.1"
                     /translation="MLELNPYYNRIKDLEGRAAALRGYLDFDEKHDRLVEVTRELEQP
                     NIWDDPERAQALGRERAALERVVLTLESLGQALKDAAELLDMAAEEQDEATVGEVAKD
                     LDRYEHQVADLEFRRMFSGEADPCNAFLDIQAGAGGTEAQDWADMLMRMYLRWGERRG
                     FKTEILEVSDGEVAGIKSCTIRFEGEYAYGWLRTETGVHRLVRKSPFDSDNRRHTSFT
                     SVFVSPEIDDRIEIEINPADLKVDTFRASGAGGQHVNRTESAIRITHVPSGIVVACQA
                     DRSQHKNRSTAMKQLQSKLYEMEMQKRRAASQALEDTKADIGWGSQIRSYVLDQSRIK
                     DLRTSHETGNPQAVLDGDLDPFIEASLKSGL"
     gene            2045..>3537
                     /gene="lysS"
                     /locus_tag="PK144_16785"
     CDS             2045..>3537
                     /gene="lysS"
                     /locus_tag="PK144_16785"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNF67429.1"
                     /translation="MTEPAVTPEAAANDETNENKLIAERRQKLAKLREAGNAFPNDFR
                     RDALAGALQGECAALDGPALDAAARRVTVAGRMMAKRIMGKASFTAIKDMSGQIQLFV
                     QRDAVGDDAYTAFKGWDVGDIVGAEGVLMRTKTGELSVRVESIRLLTKSLRPLPEKFH
                     GLTDQEVRYRQRYVDLIMNDDTRRTFAIRNRVIRGIRDYLDARGFMEVETPMMHVIPG
                     GAAARPFVTHHNALDMALYLRIAPELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPE
                     FTMIEFYQAYADYRDLMDLTEDLLRGLALELLGSTDVPYQGEVYDFGKPFARLTIPEA
                     VVAYNEGVTAEDIATLEGARAVAERLKLPVKPGDGIGKVVTTIFEETVEHQLRHPTFI
                     TAYPTEVSPLARRNDDNPEVTDRFEFFIGGREIANGFSELNDAEDQAERFREQVRQKD
                     AGDHEAMHYDADYVRALEFGLPPTAGEGIGIDRLVMLFADQPSIREV"
BASE COUNT          647 a         1239 c         1177 g          474 t
ORIGIN      
        1 aggcgcgcgt catcagcaac tggccgagcg tgccgagcag cgcaagggcg gcgatgatgc
       61 cggaatcgcg cacaccgggc acgacgccgg cggcgagcgc ggccggcgtg gtcagcagcg
      121 tgccgaccag cgtgaaccag aacacgatct cgccggcgcc gatgccgatc gacatgcgcc
      181 ggatggtcac gcgcgccagt gcggcgagcg cgccgcttgc gaggcctacc agcgccgccg
      241 gcacgaacag cccggagccg ggcttcagga tcgccagcac gccggcgaag ccgatcagcg
      301 ccgcccaggc gatgcgcggc ccgagccgct cgccgagcca cagcgccgcg atcagcggca
      361 cgaacagcgg cgaggtcatc ttcagcaccg cggcatcggc gagcggcagg tggccgaccg
      421 cccagaacga ggtccacatc gccgccaggc cggtcagcga gcgcgcggcg tgaaagcgca
      481 gcttcgccgg cgtctggcgg ccgatccagc cgcgctgcca ggcgatcggc agcagcagcg
      541 cgagcgccag ccagtggcgg atggcgagga tggtcgcggt cggcacgttc accgaggcga
      601 gcttgatcag cgcgttcatc gccgcgaacg tcgcttcgga ggcgagcagc agcagcgccc
      661 cggcgagcgg ggcatcggtc cggttcatgc ggcggcggcc gtcgggcaaa gggcgtgcag
      721 catacgggag tcgcgcggcg atcgcgtggt gcgggggcgg ccattgtgcg gccgtcgccc
      781 ggtgcaaact ccgatagaat gcgcggcttt ccccgccatg cggcctttcc ggggccgccg
      841 agacgagcgc gacgccgatg ctggaactga atccgtacta caaccgcatc aaggacctcg
      901 aagggcgcgc cgccgccctc agggggtatc tttgacttcg acgagaagca cgatcgcctg
      961 gtcgaggtga cgcgcgaact cgaacagccg aacatctggg acgacccgga gcgcgcacag
     1021 gcgctcggcc gtgaacgcgc agccctcgaa cgcgtggtgc tgacgctgga atcgctcggc
     1081 caggccctga aggacgccgc cgaactgctc gacatggccg ccgaggaaca ggacgaggcg
     1141 acggtcgggg aggtcgcaaa ggacctcgat cgctacgagc atcaggtcgc cgatctcgaa
     1201 ttccggcgca tgttctcggg cgaggccgac ccctgcaacg ccttcctcga catccaggcc
     1261 ggcgccggcg gcaccgaggc gcaggactgg gccgacatgc tgatgcgcat gtatctgcgc
     1321 tggggcgagc gccgcggctt caagaccgaa atcctcgaag tctcggacgg tgaagtcgcc
     1381 ggcatcaaat cctgcacgat ccgcttcgag ggcgagtacg cctacggctg gctgcgcacc
     1441 gagaccggcg tgcaccgtct ggtgcgcaag agtccgttcg attccgacaa ccgccgccac
     1501 accagcttca cgtcggtgtt cgtgtcgccg gaaatcgacg accgcatcga gatcgagatc
     1561 aacccggccg atctgaaggt cgacaccttc cgcgcctccg gtgccggcgg ccagcacgtc
     1621 aaccgcaccg aatcggcgat ccgcatcacg cacgtgccct cgggcatcgt cgtcgcctgt
     1681 caggccgacc gctcgcagca caagaaccgc tcgacggcga tgaagcagct gcagtccaag
     1741 ctctacgaga tggagatgca gaagcgccgc gccgcctcgc aggccctcga agacaccaag
     1801 gccgacatcg gctggggcag ccagatccgc tcctacgtgc tcgaccagtc gcgcatcaag
     1861 gatctgcgca ccagccacga gaccggcaat ccgcaggccg tgctcgatgg cgacctcgat
     1921 ccgttcatcg aggcgagcct caagagcgga ctctgagcct cccgcccgtc cctttcccga
     1981 tcagcccgcc gactcgtcgc accgccccgt cgcctgcacg gatcagcccc cggaaccgac
     2041 cgacatgacc gaacccgccg tcacccccga agccgctgcc aacgacgaaa ccaacgagaa
     2101 caagctgatc gccgagcgcc gccagaagct cgcgaagctg cgcgaagccg gcaatgcctt
     2161 cccgaacgac ttccgccgcg acgcgctcgc cggcgcgctg cagggcgaat gcgccgcgct
     2221 cgacggcccg gcgctcgacg ccgccgcccg gcgcgtcacg gtcgccggac gcatgatggc
     2281 caagcgcatc atgggcaagg cgagcttcac ggcgatcaag gacatgagcg gccagatcca
     2341 gctgttcgtg cagcgcgacg cggtcggtga tgacgcctac acggcgttca agggctggga
     2401 tgtcggcgac atcgtcggtg ccgagggcgt gctgatgcgc acgaagaccg gcgagctgtc
     2461 ggtgcgcgtc gagtcgatcc ggctgctgac caagtcgctg cgcccgctgc ccgagaagtt
     2521 ccacggcctg accgatcagg aagtccgcta ccgccagcgt tacgtcgacc tgatcatgaa
     2581 cgacgacacg cggcgcactt tcgcgatccg caaccgcgtg atccgcggca tccgcgatta
     2641 cctcgacgcg cgcggcttca tggaagtcga gacgccgatg atgcacgtga tcccgggcgg
     2701 cgccgcggcg cggcccttcg tcacgcatca caacgcgctc gacatggcgc tgtacctgcg
     2761 catcgcgccg gagctgtacc tgaagcgcct ggtggtcggc ggcttcgagc gcgtctacga
     2821 gatcaaccgc aatttccgca acgaggggct gtcgacgcgc cacaaccccg agttcacgat
     2881 gatcgagttc tatcaggcct acgccgatta ccgcgacctg atggatctga ccgaagacct
     2941 gctgcgcgga ctcgcgctcg aactgctcgg cagcaccgac gtgccgtatc agggcgaggt
     3001 ctacgacttc ggcaagccgt tcgcgcgcct gacgatcccg gaggcggtcg tcgcctacaa
     3061 cgaaggtgtc acggcggaag acatcgccac gctcgaaggc gcacgcgcgg tggcagagcg
     3121 cctgaagctg ccggtcaagc ccggtgacgg catcggcaag gtggtgacga cgatcttcga
     3181 ggaaaccgtc gagcaccagt tgcggcatcc gaccttcatc accgcgtacc cgaccgaggt
     3241 ctcgccgctc gcgcgccgca acgacgacaa ccccgaagtg accgaccgct tcgagttctt
     3301 catcggcggg cgcgagatcg ccaacggttt ttcggaactc aacgacgccg aggaccaggc
     3361 cgagcgcttc cgcgagcagg tgcgccagaa ggatgcgggc gatcacgagg ccatgcacta
     3421 cgatgccgac tatgtccggg ccctggaatt cggcctgccg ccgaccgccg gcgaaggcat
     3481 cggcatcgac cggctggtga tgctgttcgc cgatcagccg tcgatccgcg aggtcat
//
LOCUS       DAOQIE010000363         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16805_length_3533_cov_6.880391, whole genome shotgun sequence.
ACCESSION   DAOQIE010000363 DAOQIE010000000
VERSION     DAOQIE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16805_length_3533_cov_6.880391"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK144_16790"
     CDS             <1..603
                     /locus_tag="PK144_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNF67430.1"
                     /translation="KQVPFTIRYIDLDNPPDWFLALSPLGKVPLLSVGDTVLFESAVI
                     AEYLDETHPPALMPADPLARAQARAWIEFASNLLGRQYRMLVAKDQAGFDEEFAAAHR
                     ELAFVERAHADGPMFLGGDTLSLVDAGFAPFFMRFELMNRARPLALLDDYPKLRRWAA
                     ALADRDSVRRSVPEDFNDGFRRRFAGGWFGTAFADTAAMH"
     gene            779..1606
                     /locus_tag="PK144_16795"
     CDS             779..1606
                     /locus_tag="PK144_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF67431.1"
                     /translation="MKTTNHGSTAIAWQDAGHGEPALLCLPGWCCEQTVFADFVRLAA
                     PHRRTLTLDWRGHGASAAADAEFGLDGLLEDALAVIEASGVTQVVPVASSHAGWVAIE
                     LRRRLGERVPALVLIDWLVMEPPSAFFGALNGLRDAQAWQDTRDLLLAMWLPEGVSPA
                     LAQQIRAQTGRHDASMWARAAHSIAAAYRAFGTPMRALSELPDPPPVLHLASRGAPVE
                     ALREQQVFASVHPWFTVHRLAARSHFPMLEAPAATAAEVEGFLQRVQGWESVDLGCA"
     gene            complement(1744..2184)
                     /locus_tag="PK144_16800"
     CDS             complement(1744..2184)
                     /locus_tag="PK144_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010172712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF67432.1"
                     /translation="MRWYPHVTVAAIVEREGRFLFVEEDKGHGPVLNQPAGHLEDDET
                     IPAAVEREVLEETGWTVAAQALVGIYRWRNPGNGETFLRFCLACTPLAHDAQRRLDDG
                     ILRALWLSPDELCAYRLRSPLVRPCLDDWLAGERLPLTLLRTIG"
     gene            2352..2684
                     /locus_tag="PK144_16805"
     CDS             2352..2684
                     /locus_tag="PK144_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNF67433.1"
                     /translation="MSLENQNCVPCQGGIPALSRAEAERFLVDLPKWTLEGGATRIRR
                     RYAFPDFVSALAFINRVGEVAEAQGHHPDITFGWGYAEVVIFTHKIAGLHENDFILAA
                     RVEALYGG"
     gene            complement(2972..3268)
                     /locus_tag="PK144_16810"
     CDS             complement(2972..3268)
                     /locus_tag="PK144_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF67434.1"
                     /translation="MPVLTLMPSGKTVEAAAGTSILAALLAADVQIGHKCGGKAECDG
                     CHVFVLEGRKSLSRIQRLENERLDTIVGVGSKSRLACQAILGEENVTVELLSFV"
BASE COUNT          528 a         1190 c         1255 g          560 t
ORIGIN      
        1 aaacaggtgc cgttcacgat ccgctacatc gacctcgaca acccgccgga ctggttcctg
       61 gcgctgtcgc cgctcggcaa ggtgccgctg ctcagcgtcg gcgacaccgt gctgttcgaa
      121 tcggccgtga tcgccgaata cctcgacgag acgcatccgc cggcgctgat gccggccgat
      181 ccgctggcgc gcgcgcaggc ccgcgcctgg atcgagttcg catcgaacct gctcggccgc
      241 cagtaccgga tgctggtcgc gaaggatcag gccggcttcg acgaggaatt cgccgccgca
      301 caccgcgaac tcgccttcgt cgagcgtgcg cacgccgacg gaccgatgtt cctcggcggc
      361 gacacgctgt cgctcgtcga tgccggcttc gcgccgttct tcatgcgctt cgagctgatg
      421 aaccgggcgc gaccgctggc gctgctcgat gactatccga agctcaggcg ctgggcggcg
      481 gcgctcgccg atcgggattc ggtgcgccgc tcggtgccgg aggacttcaa cgacggcttc
      541 cgccggcgct ttgccggagg ctggttcggt acggcattcg cagatacggc agccatgcac
      601 tagattgatg cgaaacggga actcgctgca ccagaatagg ctcagttcct gaaacagcgc
      661 accggaccca cgcccatcgc gtcctcaccg gtgccaaagc caacggccgg ccggctcgct
      721 cccgccggcc gttgctgtga ccggcgagta ccgccaggag atagaggacc gtgatccaat
      781 gaaaaccacg aatcatggca gtaccgcgat cgcctggcag gacgccggcc atggcgagcc
      841 tgccctgctg tgcctgccgg gctggtgctg cgagcagacg gtgtttgccg acttcgtccg
      901 gcttgccgcc ccgcaccggc gcacgctgac gctggactgg cgcggccacg gcgcctcggc
      961 cgcggccgac gcggagttcg gcctcgacgg cctgctggag gatgcgctgg cggtgatcga
     1021 ggcgagcggg gtgacgcagg tcgtgccggt ggcgagttcg cacgccggct gggtggcgat
     1081 cgagctgcgc cggcggctcg gcgagcgcgt gccggcgctg gtgctgatcg actggctggt
     1141 catggagccg ccctcggcgt tcttcggcgc gctgaacggc ctgcgcgatg cgcaggcctg
     1201 gcaggacacg cgcgacctgc tgctggcgat gtggctgccc gaaggggtgt cgccggcgct
     1261 cgcgcagcag atccgcgcgc agacggggcg tcacgatgcg tcgatgtggg cgcgcgcggc
     1321 ccattcgatc gcggccgcct accgggcctt cggcacgccg atgcgggcgc tgtcggagct
     1381 gccggacccg ccgccggtgc tgcatctggc ctcgcggggc gcgccggtcg aggcgctgcg
     1441 cgagcagcag gtgttcgcgt cggtgcatcc gtggttcacc gtgcatcgcc tcgcggcccg
     1501 atcgcacttc ccgatgctcg aagccccggc cgcgacggcg gccgaggtcg aaggcttcct
     1561 gcagcgcgtg cagggctggg aatcggtcga tctcggctgc gcgtgaagcc ggcagtctgt
     1621 gccccccgga gccgccgcag cggcttcggg acggcaggcc gcgaacgctt tgcgctgacg
     1681 gggcggccag cgtcgccggt cggcccggct gccggtgcgc agcgcagcct tccgggggcc
     1741 cgctcagccg atcgtgcgca gcagcgtcag cggcaggcgc tcgccggcca gccagtcatc
     1801 gaggcagggg cgcaccagcg ggctgcgcag ccggtaagcg cagagttcat cggggctgag
     1861 ccacagcgcg cgcaggatgc cgtcatcgag ccggcgctgc gcatcgtgcg caagcggcgt
     1921 gcaggcgagg cagaaacgca ggaaggtctc gccgttgccg ggattgcgcc agcggtagat
     1981 gccgaccagc gcctgcgccg ccaccgtcca gccggtttcc tccagcacct cgcgctcgac
     2041 ggctgccggg atggtttcgt cgtcctccag atgcccggcc ggctggttca gcaccggccc
     2101 gtggcccttg tcctcctcga cgaacaggaa acggccctcg cgctcgacga tcgcagcgac
     2161 ggtgacgtgc ggataccagc gcatcggggg tactcctgag ctatggattg agttatggat
     2221 gaggggtgaa ccctcggacc gccgcgggcg gccgggttcg ctttccgggg gccggcctgc
     2281 ggccccgatc ctgcccgcgg aggtccggac ccgacttccg cgcggcaccc gagcgtaggg
     2341 agcccatcgc catgtccttg gaaaatcaga actgcgtgcc gtgccagggg ggcattccgg
     2401 ccctgagccg cgccgaagcc gaacgcttcc tcgtcgatct gccgaagtgg acgctcgaag
     2461 gcggcgccac gcgcatccgc cgtcgttatg ccttcccgga cttcgtcagc gcgctggcct
     2521 tcatcaaccg cgtcggcgag gttgccgagg cccagggcca tcatccggac atcaccttcg
     2581 gctggggcta cgccgaggtc gtgatcttca cgcacaagat cgccggcctg cacgagaacg
     2641 acttcatcct cgccgccagg gtcgaggcgt tgtacggcgg ctgagccgcg aggcatccgg
     2701 cctgcggtgc atgcttcatg aatcacgatg caccggccat gactcacgat tcaggcttca
     2761 tgacaggcga cgggcaagcc gtccatcgcc tgtcatgcgc cagacccgtg ccgcaggacg
     2821 aaccggtgca ggcacaggga gcgtggcgga gcgccggtct ctgcatcgat cgggcttgcg
     2881 aagggccgga caggcgtcat cggcctgtcc ggacgctgcg ctggcaggcc cggaccggtc
     2941 gcgggcagcg gaccggccgg gcggcgcagg ttcagacgaa gctcagcagt tcgaccgtga
     3001 cgttctcttc accgaggatc gcctgacagg cgagccgcga cttcgagccg acaccgacga
     3061 tggtgtcgag ccgctcgttc tcgaggcgct ggatgcgcga gaggctcttg cggccttcca
     3121 gtacgaacac atggcaacca tcacattcgg ccttgccgcc gcacttgtgg ccgatctgca
     3181 catcggcagc gagcagggcg gcgaggatgc tggtaccggc ggcggcttcg acggtcttgc
     3241 ccgaaggcat gagggtgagg acgggcatgg gcatgcgctc ctgggtttgt acgggcttgg
     3301 cagcgtctgt tttgtcggaa caacgacaag acttcccgcg cgccggcgca gggagccgtc
     3361 gaaacaattc cgtcagggat caatggattg catgctggcc gtatgacagg cgccggtacc
     3421 tgcggggccg gcaggcgatc ccgcagggga ggtgagcaaa tgacgtgccg ggatggaggt
     3481 cagggtcgtg caacgctacc tgattccttt gtaattcaga gcattgtcaa gac
//
LOCUS       DAOQIE010000364         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16816_length_3532_cov_5.805867, whole genome shotgun sequence.
ACCESSION   DAOQIE010000364 DAOQIE010000000
VERSION     DAOQIE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16816_length_3532_cov_5.805867"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PK144_16815"
     CDS             <1..501
                     /locus_tag="PK144_16815"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNF67435.1"
                     /translation="RLDAALARGVRLVQLRAKALPAGALAPLCAAALQCCRAAGARLL
                     LNGAPALAMQLGADGVHLDARALAACDRRPLPAGRLLAASCHDAHELAHAQRIGCDFA
                     VLGPLAATATHPEAEPLGWARFAALVRTAGLPVYALGGLGPADLDAAWAAGAQGVAAI
                     RALWQP"
     gene            498..992
                     /locus_tag="PK144_16820"
     CDS             498..992
                     /locus_tag="PK144_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67436.1"
                     /translation="MSPGARRLGIVAALLALAAAGTGLGIALPKLRAWLYGDAPAIEL
                     TRDDTACRPIGVPCSATHARLQLALGLGQSVSPLQAFPVTVSVQGAEAAGLRSVELAF
                     TMQGMDMGILRTPLLRQADGSWRGRGLLPVCAAGRSDWLATVTATGADGERYRAQFAF
                     PAGH"
     gene            1053..>3532
                     /locus_tag="PK144_16825"
     CDS             1053..>3532
                     /locus_tag="PK144_16825"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF67437.1"
                     /translation="MRILAIRGRNLASLAGDFAVELAAPPLAQAGLFAITGPTGAGKS
                     TLLDALCLALYDEMPRLAGAPKVAVVPGADADERLNADDVRGILRRGCTEACAEVDFL
                     GHDRRRYRARWELRRARGKADGRTQNQTLVLTDLDTQQRLGGTKTETLAEIRARLGLS
                     FEQFRRSALLAQGDFASFLRARAQERAELLERITGTQLYGELSIAAHRRARDEEDALR
                     ELEQGLAALEPLDESARAELLAGHARQAEALAVLEARHLALRRLADWQTRSRTLAVEL
                     AEADRALQQARAAVDAAGLRQQVWQRIRAAQPLRPVVEAVERTRTAAEAASQRLAGAC
                     QALADADAGLRQAQAEAQAAGERHAAAEGARLQAAPQLARAHALDARLADATLHRDTA
                     VRLATGAEQAAEDAQQTLAGLQQQIGRSEAAQAEAEGWLAAHAGLDALAGQWPRWDAA
                     FRQLLDRLREHEDARRLQAQAAHEQTTAAAALADAEQLREQARAARLQAEAEAGRLAA
                     ALAAIDADGLAARRRALAVRREALQPLPAMVERAGVLATQAQALVARIDDWRTREQQL
                     GSALDAAETALPALRARAEEAELAARRAALAAAADVGHLRAQLEDGAPCPVCGSGEHP
                     WARALPALDRLCGETRRHADSLREQCEAAAASASMRQLQRTEARTRLAEAQAEAGRLA
                     ADRADLQARWQQRPAGENLPGDLLQPGLADALASRLAALKVDVDALEAQEAEARRLAQ
                     TARQAQQALTEARAAQETAEAGLTRCRGETERIERLGADAQAAGERAACAAEALYAEL
                     AAPCSGLPAAAARLPQAPAALHAEAAAAV"
BASE COUNT          429 a         1356 c         1369 g          378 t
ORIGIN      
        1 cggctcgatg cggccctcgc gcgcggcgtg cggctcgtgc agctgcgcgc gaaggcgctg
       61 ccggccgggg cgctcgcgcc gctgtgtgcg gcggcgctgc aatgctgccg cgcggccggt
      121 gcgcggctgc tgctgaacgg cgcaccggcg ctggcgatgc agctcggggc cgatggcgta
      181 cacctcgatg cgcgcgcgct cgccgcctgc gaccggcgcc cgctaccggc cggccggctg
      241 ctcgcggcct cctgccacga tgcgcacgaa ctcgcacacg cacagcgcat cggctgcgac
      301 ttcgccgtgc tcggcccgct cgccgcgacc gcgacccacc cggaggccga accgctcggc
      361 tgggcgcgct ttgccgcgct cgtgcgcacg gccggcctgc cggtgtacgc gctcggcggc
      421 ctcggcccgg ccgatctcga tgccgcgtgg gcggccggcg cgcagggcgt cgcggcgatc
      481 cgcgcgctgt ggcagccgtg agtccgggcg cgcgccggct cggcatcgtc gccgcgctgc
      541 tggcgctggc cgcggccggc acggggctcg gcatcgcgct gccgaagctg cgcgcctggc
      601 tgtacggcga cgccccggcc atcgagctga cccgcgatga caccgcctgc cggccgatcg
      661 gcgtgccgtg ctcggcgacg cacgcgcgcc tgcagctcgc gctcggcctc gggcagtcgg
      721 tctcaccgct gcaggcgttt ccggtcaccg tcagcgtgca gggtgccgag gccgccgggc
      781 tgcgctcggt cgagctggcg ttcacgatgc agggcatgga catgggcatc ctgcgcacgc
      841 cgctgctgcg tcaggccgac ggcagctggc gcgggcgcgg gctgctgccg gtgtgcgcgg
      901 ccgggcgcag cgactggctc gcgaccgtga ccgccaccgg cgccgatggc gagcgctacc
      961 gcgcgcagtt cgcgttcccg gccgggcact gaacgcaagc ccggcaccgt catccgcttc
     1021 gccgtcacct cgccgccaca gggacagtcc gcatgcgcat cctcgcgatc cggggccgca
     1081 acctcgccag tctggccggc gacttcgccg tcgagctcgc cgcgccgccg ctggcgcagg
     1141 ccgggctgtt cgcgatcacc ggcccgaccg gggccggcaa gtcgacgctg ctcgatgcgc
     1201 tgtgcctggc gctgtatgac gagatgccgc gcctcgccgg tgcgccgaag gtcgcggtcg
     1261 tgcccggcgc ggatgccgat gagcggctca acgccgatga cgtgcgcggc atcctgcgcc
     1321 gcggctgcac cgaggcctgc gccgaggtcg atttcctcgg ccacgaccgc cggcgctacc
     1381 gcgcgcgctg ggagctgcgc cgcgcccgcg gcaaggccga tggccgcacc cagaaccaga
     1441 cgctggtgct caccgatctc gacacgcagc agcgcctcgg cggcacgaag accgagacgc
     1501 tggccgaaat ccgcgcccgg ctcggcctga gcttcgagca gttccggcgc agcgcgctgc
     1561 tcgcgcaggg tgacttcgcg agcttcctgc gcgcccgggc ccaggagcgc gccgagctgc
     1621 tggagcgcat caccggcacg cagctctacg gcgaactgtc gatcgccgcg caccgccgcg
     1681 cgcgcgacga ggaagacgcg ctgcgcgaac tggaacaagg cctcgccgcg ctcgaaccgc
     1741 tggacgagtc cgcgcgggcc gaactgctcg ccgggcacgc acggcaggcc gaagcgctgg
     1801 ccgtgctcga agcccggcac ctcgcgctgc gccgcctcgc cgactggcag acgcgcagcc
     1861 gcacgctcgc cgtcgaactc gccgaagcgg atcgggcact gcagcaggcc cgggccgccg
     1921 tcgatgcggc cgggctgcgg cagcaggtct ggcagcggat ccgggccgcc cagccgctgc
     1981 gcccggtggt cgaggccgtc gagcgcacgc gcaccgcggc cgaagcggcg agccagcggc
     2041 ttgccggggc atgccaggcg ctggcggacg ccgatgccgg gctgcggcag gcgcaggcag
     2101 aagcgcaggc cgccggcgag cggcatgcag cggcagaggg cgcccgcctg caggcagcgc
     2161 cgcagctcgc ccgcgcccat gccctcgatg cccggctcgc cgatgccacc ctgcaccggg
     2221 acacggccgt gcggctggcg accggggctg agcaggcggc cgaagatgcg cagcagacgc
     2281 tcgccggcct gcagcagcag atcgggcgca gcgaggccgc gcaggccgaa gccgagggct
     2341 ggctggccgc gcacgccggc ctcgacgcac tggccggcca gtggccgcgc tgggatgccg
     2401 cgttccggca gttgctcgat cggctgcgcg agcacgagga cgcccgccgc ctgcaggcac
     2461 aggccgcgca cgagcagacg acggccgccg ccgcgcttgc agatgcggag cagcttcgcg
     2521 aacaggcccg ggccgcgcgg ctgcaggccg aagccgaagc cggcaggctg gcggctgcgc
     2581 tcgcagcgat cgatgcagac ggactcgcgg cccgccgccg ggcactcgcg gtgcggcgcg
     2641 aagccctgca gccgctgccg gccatggtcg agcgcgccgg cgtgctggcc acgcaggcgc
     2701 aggcgctggt cgcgcgcatc gacgattggc ggacgcggga gcagcagctc ggcagcgccc
     2761 tcgatgccgc agagaccgcc ttgccggccc tgcgcgcgcg tgccgaagaa gccgagctgg
     2821 cggcgcggcg ggcggcgctg gcggcggcgg ccgatgtcgg acacctgcgg gcgcagctcg
     2881 aagacggcgc gccgtgcccg gtctgcggct ccggcgagca tccgtgggcg cgggcgctgc
     2941 cggcgctcga tcgactgtgc ggggaaaccc gccggcatgc cgacagcctg cgcgagcaat
     3001 gcgaggccgc ggctgcgagc gcgtccatgc ggcagctgca gcggaccgag gcgcgtacgc
     3061 gtctcgccga ggcgcaggcc gaagccgggc ggctcgccgc ggaccgggcc gacctgcagg
     3121 cgcgctggca gcagcgtccg gccggcgaga acctgcccgg ggatctgctg cagcccggcc
     3181 tggccgatgc gctcgcctcc cggctggccg ccctgaaggt cgacgtggac gcgctggagg
     3241 cgcaggaagc cgaggcccgc cggctggcac agacggcccg gcaggcgcag caggcgctga
     3301 cggaagctcg tgccgcccag gaaaccgccg aggccggact gacccgctgc cgcggcgaga
     3361 ccgagcgcat cgagcggctc ggcgcggacg cgcaggcggc cggcgagcgg gccgcctgcg
     3421 cggcagaggc gctgtacgcg gaactcgcag caccgtgctc gggcctgccg gcggcggctg
     3481 cacgcctgcc gcaggcaccg gcggccctgc acgccgaggc cgccgccgcg gt
//
LOCUS       DAOQIE010000365         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16896_length_3522_cov_6.665994, whole genome shotgun sequence.
ACCESSION   DAOQIE010000365 DAOQIE010000000
VERSION     DAOQIE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16896_length_3522_cov_6.665994"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK144_16830"
     CDS             complement(<1..196)
                     /locus_tag="PK144_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNF67438.1"
                     /translation="MSTADRNIRAAPRIIAAPDSWIEGAAVQQLEAVAALPGMQRAVG
                     MPDLHPGKVTPIGAAFASVGL"
     gene            complement(208..1437)
                     /locus_tag="PK144_16835"
     CDS             complement(208..1437)
                     /locus_tag="PK144_16835"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNF67439.1"
                     /translation="MSSKTELSHEVMNVPDGVPVKMWTRGVPVDDKARAQLANTAQMP
                     FIHKWIAVMPDVHVGKGSTIGSVIPTKGAIIPAAVGVDIGCGMMAAKTTLNARDLPDD
                     LLPLRRAIERAVPHGRSPGARDPGAWHEPPTAVDAAWTALAGRFAAITAKYPKLAKAN
                     HRVHLGTLGTGNHFIEVCLDEDEAVWFLLHSGSRGIGNAIGTFFIELAREDMRRWLIN
                     LPDRDLAYLPEGTAHFDDYVEAVEWAQDFARANRELMMQAVVDAARAVISKPFEARCE
                     VVNCHHNYVSREHHYGENILVTRKGAVRARAGELGIIPGSMGAKSFIVRGLGNPESFE
                     SCSHGAGRVLSRSEAKRRFTLADQVRETAGVECRKDTDVIDEIPSAYKDIDAVMHAQR
                     DLVEVVHTLKQLVCVKG"
     gene            1663..3294
                     /gene="rtcR"
                     /locus_tag="PK144_16840"
     CDS             1663..3294
                     /gene="rtcR"
                     /locus_tag="PK144_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HNF67440.1"
                     /translation="MSRNRPLVVIGLLGTTLDSGKGAGRWEKWRPSVALCQQPDCVID
                     RFELLYEARFRELAEHVASDIGFISPETTLTLHALTMSDPWDFEEVYGALHDFARGYP
                     FAPEAEDYLVHITTGTHVAQICLFLLTEARELPARLLQTAPPRKQIASDPGSCAVIDL
                     DLSRYDRIARRFAREQVEQADFLKAGIATRNPAFNTTIARIERVALRSRAPLLLLGPT
                     GAGKSFLARRIYELKRARHRLSGAFVEVNCATLRGDGAMSALFGHKRGAYTGAQTERA
                     GLLRSADKGLLFLDEIGELGPDEQAMLLKAIEDGRFLPVGADKEVESRFELIAGTHRD
                     LAAEVAAGRFREDLYARINLWTFRLPGLAERREDIEPNLDFELERHAREQGQRVRFNA
                     EARRRYLDFATGPDAPWRGNFRELGASVLRMATLAEGGRIDVDTVAEELDRLRGQWGE
                     RTEEESAGDDPVSALLGVEAAAGLDRFDRVQLAEVLRICRGSASLSAAGRELFAVSRA
                     GKANPNDADRLRKYLARFGLEWGRVRGGWVKSGAG"
BASE COUNT          522 a         1178 c         1240 g          582 t
ORIGIN      
        1 tcagcccgac gctcgcaaac gccgcaccga tcggcgtgac cttgccgggg tgcagatcgg
       61 gcatgccgac cgcacgctgc atgccgggca gcgcagccac ggcttcgagc tgctgcacgg
      121 ccgcgccttc gatccagctg tcgggggcgg cgatgatgcg gggggcagcg cggatattgc
      181 ggtcggccgt gctcatcatg tccatcctca gcccttcacg cacacgagct gcttcagcgt
      241 gtgcacgacc tcgaccagat cgcgctgcgc gtgcatcacc gcgtcgatgt ccttgtacgc
      301 ggacggaatc tcgtcgatca cgtcggtgtc cttgcggcac tcgacgcctg cggtctcgcg
      361 tacctggtcg gcgagcgtga agcggcgctt ggcctcgctg cgcgacagca cgcgaccggc
      421 gccgtggctg caggactcga agctctccgg gttgccgagg ccgcgcacga tgaagctctt
      481 cgcgcccatg ctgcccggaa tgatgccgag ttccccggcg cgcgcgcgca ccgcgccctt
      541 gcgggtgacg aggatgtttt cgccgtagtg gtgctcgcgg gagacgtaat tgtgatggca
      601 gttcacgact tcacagcgcg cctcgaacgg cttcgaaatc accgcccgcg ccgcatccac
      661 gaccgcctgc atcatcagtt cgcggttggc gcgcgcgaag tcctgcgccc actcgacggc
      721 ctcgacgtaa tcgtcgaagt gcgccgtgcc ttccggcaga taggcgagat cgcgatccgg
      781 caggttgatc agccagcggc gcatgtcctc gcgtgccagc tcgatgaaga acgtgccgat
      841 ggcgttgccg atgccgcgcg agcccgagtg cagcaggaac cacacggcct cgtcctcgtc
      901 gaggcagacc tcgatgaagt ggttgccggt gccgagcgtg ccgagatgca cgcggtggtt
      961 ggccttcgcg agcttcgggt atttggcggt gatcgccgcg aagcgcccgg caagcgcggt
     1021 ccacgcggca tcgacggcgg tcggcggctc gtgccaggcg cccggatcgc gcgcgcccgg
     1081 cgagcggccg tgcggcacgg cgcgctcgat cgcgcggcgc agcggcagca ggtcgtcggg
     1141 caggtcgcgg gcgttcagcg tcgtcttcgc cgccatcatc ccgcagccga tatcgacgcc
     1201 gaccgccgcc ggaatgatcg cgcctttggt cgggatcacg ctgccgatcg tcgaaccctt
     1261 gccgacgtgc acgtccggca tcaccgcgat ccacttgtgg atgaacggca tctgcgcggt
     1321 gttggcgagc tgcgcacgcg ccttgtcatc gaccggcacg ccgcgcgtcc acatcttcac
     1381 cggcacgccg tccgggacgt tcatgacttc gtggctgagt tcggtcttgc tgctcatcgc
     1441 gggactcctc gccggctcgt tgcgccggca gtcgtggcgt cgggggatgg ccccggtatc
     1501 gcaggcagcg tgccagttgc gggcttcgcg ctgcagcagg ctgatccggc agggatttgc
     1561 gtggccggtg cgctgccgca agggcttcca tggcgcacgg gtttctgatc cgtacggatc
     1621 aggatttatc ctgatggctc ctgtcccccg gaagcctgcc tcatgtcccg gaaccgtccg
     1681 ctggtcgtca tcggtctgct cggcaccacg ctcgacagcg gcaagggcgc cgggcgctgg
     1741 gagaagtggc gcccgagcgt ggcgctgtgc cagcagccgg attgcgtgat cgaccgtttc
     1801 gagctgctgt acgaggcgcg cttccgcgag ctggccgaac acgtcgccag cgacatcggt
     1861 ttcatctcgc ccgagacgac gctgacgctg cacgcgctga cgatgagcga tccgtgggat
     1921 ttcgaggagg tctacggcgc gctgcacgac ttcgcgcgcg gctatccgtt cgcgcccgag
     1981 gccgaggatt acctcgtgca catcacgacc ggcacgcacg tcgcgcagat ctgcctgttc
     2041 ctgctgaccg aagcgcgcga gctgccggcg cggctgctgc agacggcgcc gccgcgcaag
     2101 cagatcgcca gcgatcccgg cagttgcgcg gtgatcgacc tcgacctgtc acgttacgac
     2161 cgcatcgccc ggcgcttcgc gcgcgagcag gtcgagcagg cggatttcct gaaagccggc
     2221 atcgccaccc gcaatccggc cttcaacacg acgatcgcgc gcatcgaacg tgttgcgctg
     2281 cgctcgcgcg cgccgctgct gctgctcggg ccgaccggcg ccggcaagag ctttctggcg
     2341 aggcgcatct acgaactcaa gcgcgcccgc caccggctga gcggtgcctt cgtcgaggtc
     2401 aactgcgcga cgctgcgcgg cgatggcgcg atgtcggcgc tgttcggcca caagcgcggt
     2461 gcctacaccg gcgcgcagac cgaacgcgcc ggcctgctgc gcagtgccga caaggggctt
     2521 ctgttcctcg acgagatcgg cgaactcggg ccggatgagc aggcgatgct gctgaaggcg
     2581 atcgaggacg gccgcttcct gccggtcggc gccgacaagg aagtcgaaag ccgcttcgag
     2641 ctgatcgccg gcacgcaccg cgatctggcc gccgaggtcg cggccgggcg cttccgcgag
     2701 gatttatacg cgcggatcaa tctgtggacc ttccggctgc cgggcctggc cgagcggcgc
     2761 gaggacatcg agccgaacct cgacttcgaa ctcgaacgcc acgcgcgcga gcaaggccag
     2821 cgcgtgcgct tcaacgccga agcccgccgc cgctacctcg acttcgcgac cggcccggat
     2881 gcaccgtggc gcggcaactt ccgcgaactc ggcgcctcgg tgctgcgcat ggcgacgctg
     2941 gccgagggcg ggcgcatcga cgtggacacg gtcgccgagg aactcgaccg gctgcgcggg
     3001 cagtggggcg agcggacgga ggaggagtcc gccggcgacg acccggtcag cgcactgctc
     3061 ggcgtcgaag ccgcggcggg gctcgatcgc ttcgatcgcg tgcagctcgc cgaggtgctg
     3121 cgtatctgcc gtggcagcgc atcgttgtcg gccgccgggc gcgagctgtt cgcggtttcc
     3181 agagcgggca aggcaaatcc gaatgatgcg gaccggctgc gcaagtatct ggcgcggttc
     3241 gggttggagt gggggagggt gcggggaggg tgggtgaaga gtggtgcggg gtgatgcgca
     3301 gcgaatgaac ggttttgccg atccctgccg gatgtcgtgg tcaagtttca ggggacatcc
     3361 cgaccggagt ttccgccaaa actggacggg gccagcaaga atgtggggaa agccatgcat
     3421 tcatgcactt gcgtgatcgg catcagccgc cggccgctac cggcgatgcg atccgcgctc
     3481 ggtccgtacc acagcaccgg tgctgatgtt gtggcgttgg aa
//
LOCUS       DAOQIE010000366         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16942_length_3516_cov_6.088414, whole genome shotgun sequence.
ACCESSION   DAOQIE010000366 DAOQIE010000000
VERSION     DAOQIE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16942_length_3516_cov_6.088414"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..2544)
                     /locus_tag="PK144_16845"
     CDS             complement(352..2544)
                     /locus_tag="PK144_16845"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNF67441.1"
                     /translation="MSHENAKQQDLARATTDERAPMSTNQGQPVGDDQNSLKAGGRGP
                     TLLEDFLLREKLQHFDHERIPERVVHARGAAAHGFFEVYDDSLSEFTTAQVLTDPSLK
                     TPVFVRFSTVAGSRGSADTARDVRGFAVKMYTPQGNWDLVGNNIPVFFIQDAIKFPDL
                     IHAAKAEPHNEIPQAATAHDTFWDFISLTPEAMHMIMWVMSDRAIPRSYALMDGFGVH
                     TFRLVNAEGVAHFVKYHWKPKHGAHSLVWDEALKLAGADPDFHRKDLWESIEAGNCPE
                     WELAVQIFDEATAAQFDFDILDATKIVPEDLVPLRRVGRMVLDRNPDNFFAETEQVAF
                     MTTNIVPGMDFTEDPLLQGRNFSYLDTQLSRLGSPNFAELPINRPLACVANHQRDAHM
                     RHTINKGRVAYAPASLDGHDPAATGAADGFHSHAVALEGRKLRERSPSFADHYGQAKL
                     FWNSQTAVERAHIAKALSFELSKVATREVRVRMLDHLQQINPVLAAQVGRALGETAGD
                     SAAAVPPSAASDKADEIAIFASAVSPTSASGRLQSTRGLSLLDGSQPASAAGRKVAVL
                     AAPGSDASQILSLRSALSCAGAQAMVVGPHLGPLTGEIAAMATFANSSSVLFDAVFVP
                     GGAPGVQQLTEIGEARRFLDEAYRHGKPIGVLAEGRALLAATQLGTLSGLTATAASGS
                     SGSPAATLEAQGVLIADGSDVATAVDRFVATLARHRFPDRPLAAQIAA"
     gene            2793..>3516
                     /locus_tag="PK144_16850"
     CDS             2793..>3516
                     /locus_tag="PK144_16850"
                     /inference="COORDINATES: protein motif:HMM:NF019956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNF67442.1"
                     /translation="MSGLPKPRVFISYSHDTPAHMARVLLLAQTLRGHGAVVELDQFH
                     GARIVNWPDWCRRQISREQSDFVICVCTAEYRRRINDSEQPEQGRGVFWEGTLMQSEL
                     YDEKRNRRFIAVLFDAEPETSIIEVLRGWTFCRLDRFALDAGGYELLYRILTGQAFVE
                     AAPVGEVIALPTDRSAPAALHATSVVVEITHLPAATPDFLGRTTELASLDEAWTDAEG
                     TEALVLVAPGGTGKSSLVRRWLE"
BASE COUNT          504 a         1173 c         1282 g          557 t
ORIGIN      
        1 ggttcgacaa ggtcaagaag ttctgggacg agatgaccgg cgcgggctga tccgctgcag
       61 tgccggcgcg ggcagggcgg tgcgggacga ttcccggctg ccgccgatgc agaccccgcg
      121 ctgggtcaga cccgaaaagc cgggcgcgca gcccggcttt tctgctgtct gcggccgacg
      181 ccttccatca gcactgccag cacaggatac cgatcccaac agaacgcctc gcttttcagc
      241 ccctcggcca tcctgcgagc tgcccccatt gtcccgcctc tgcgccggca ggtcgccgcc
      301 ggatcgtcgg ggcggcttgc gggcgcccgg cggtttccgc atgccgccgc ctcaggccgc
      361 gatctgtgcc gccagcggcc ggtccgggaa gcggtggcgg gccagggtgg cgacgaagcg
      421 atcgacggcg gtggccacgt cgctgccatc ggcgatcagc acgccctggg cttcgagtgt
      481 tgcggccggc gagccgctgc tgccggaggc ggcggtggcc gtgaggccgc tcagggtgcc
      541 gagctgggtt gcggccagca gcgcccggcc ttcggcgagc acgccgatcg gcttgccgtg
      601 gcggtaggcc tcgtcgagga agcgccgggc ctcgccgatc tcggtcagct gctgcacgcc
      661 gggggcgccg cccggcacga acacggcgtc gaacagcacc gagctgctgt tggcgaaggt
      721 cgccatcgcc gcgatctcgc cggtcagcgg gccgaggtgg gggccgacga ccatggcctg
      781 cgcgccggcg cagctgagcg ccgagcgcag cgacaggatc tggctggcat cgctgcccgg
      841 agcggcgagc accgcgacct tgcgcccggc ggccgaggcc ggctggctgc cgtcgagcag
      901 gctcaggccg cgcgtgctct gcagccggcc cgaggccgag gtcggcgaca cggccgaggc
      961 gaagatcgcg atctcgtcgg ccttgtcgct cgctgccgag ggcggcacgg ctgcggcgct
     1021 gtcaccggcc gtctcgccga gcgcgcggcc gacctgcgcg gcgagcaccg ggttgatctg
     1081 ctgcagatga tcgagcatgc gcacgcgcac ctcgcgcgtc gcgaccttgc tcagttcgaa
     1141 ggacagcgcc ttggcgatgt gcgcgcgctc gaccgcggtc tggctgttcc agaacagctt
     1201 cgcctgaccg taatggtcgg cgaagctcgg gctgcgttcg cgcagcttgc ggccttcgag
     1261 cgcgactgcg tgcgaatgga agccatcggc cgcgccggtg gccgccggat cgtggccatc
     1321 gagcgaggcc ggtgcgtagg cgacgcggcc cttgttgatg gtgtggcgca tgtgcgcgtc
     1381 gcgctggtgg ttggcgacgc aggcgagcgg ccggttgatc ggcagttcgg cgaagttcgg
     1441 cgagccgagg cgcgacagtt gcgtgtcgag gtaggagaaa ttgcgcccct gcagcagcgg
     1501 gtcttcggtg aagtccatgc cgggcacgat gttggtggtc atgaacgcca cctgctcggt
     1561 ctcggcgaag aagttgtcgg ggttgcgatc gagcaccatg cgcccgaccc ggcgcagcgg
     1621 cacgaggtcc tcgggcacga tcttggtggc atcgaggatg tcgaaatcga attgcgcggc
     1681 ggtcgcctcg tcgaagatct gcacggccag ttcccattcc gggcagttgc cggcctcgat
     1741 cgattcccac aggtccttgc ggtggaagtc cggatcggcg ccggcgagct tcagcgcctc
     1801 gtcccagacc agcgagtgcg cgccgtgctt gggcttccag tggtacttga cgaagtgcgc
     1861 gacgccctcg gcattgacga gccggaaggt gtgcacgccg aagccgtcca tcagcgcgta
     1921 gctgcgcggg atcgcccggt cgctcatgac ccacatgatc atgtgcatgg cctcgggcgt
     1981 cagcgagatg aagtcccaga aggtgtcgtg cgcggtggcc gcctgcggga tttcgttgtg
     2041 cggctcggcc ttggcggcgt ggatcaggtc cgggaacttg atcgcgtcct ggatgaagaa
     2101 caccgggatg ttgttgccga ccagatccca gttgccctgc ggcgtgtaca tcttcacggc
     2161 gaagccgcgc acgtcgcgcg cggtgtcggc cgagccgcgc gagccggcga cggtcgagaa
     2221 gcgcacgaac accggcgttt tcagggacgg atcggtcagc acctgcgcgg tcgtgaattc
     2281 cgacagcgaa tcgtcgtaga cctcgaagaa gccatgcgcc gctgcgccgc gcgcatgcac
     2341 gacgcgctcc gggatgcgct cgtgatcgaa gtgctgcagc ttctcgcgca gcaggaaatc
     2401 ctccagcagc gtcggcccgc gcccgccggc cttcagcgag ttctggtcat ccccgaccgg
     2461 ctggccctga ttcgtgctca tcggcgcccg ctcgtcggtg gtggcgcggg ccagatcctg
     2521 ctgcttggcg ttttcgtggg acatgcggaa attcctcgct cgctgggggg ccggcactgg
     2581 aaggatgtcg tcggccggtt cgacgcggac cgtgcgcggg cacacggcag ccgtgtcgtc
     2641 cggaccccgg acggtgcgcg ggggttcggc gggcggggcg gggcgggggt tacgatccgg
     2701 caaggtgggg cgggccgcag gcacgacagc cgccgccggc ctcgctacgc tgcactgcac
     2761 actccaaagc gccgcagggc agggaggctg cgatgtccgg actacccaag ccgcgggtgt
     2821 tcatcagcta cagccacgac acgcccgcgc acatggcgcg ggtgctgctg ctggcgcaga
     2881 cgctgcgcgg acacggtgcg gtcgtcgaac tcgatcagtt ccacggcgcg cgcatcgtca
     2941 actggccgga ctggtgccgg cggcagatca gccgcgagca gtcggatttc gtgatctgcg
     3001 tctgcacggc cgagtaccgg cgccggatca acgacagcga gcagccggag cagggtcggg
     3061 gcgtgttctg ggaaggcacg ctgatgcaat ccgagctgta cgacgagaag cgcaaccgtc
     3121 gcttcatcgc cgtgctgttc gacgccgaac ccgaaaccag catcatcgag gtgctgcgcg
     3181 gctggacgtt ctgccggctc gatcgctttg cgctcgacgc cggcggctac gaattgctgt
     3241 accgcatcct gaccgggcag gcttttgtcg aagcggcgcc ggtgggcgag gtgattgcac
     3301 tgccgactga tcgcagtgcg ccggcggcac tgcacgccac gtcagtcgtc gtcgaaatca
     3361 cccacctgcc cgccgccacc cccgacttcc tcggccgcac caccgaactc gctagcctcg
     3421 acgaagcgtg gaccgatgcc gaaggcaccg aggccctcgt actcgtcgcc cccggcggca
     3481 ccggcaagtc ctcgctggtg cgccgctggc tggagg
//
LOCUS       DAOQIE010000367         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_16988_length_3511_cov_5.475694, whole genome shotgun sequence.
ACCESSION   DAOQIE010000367 DAOQIE010000000
VERSION     DAOQIE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16988_length_3511_cov_5.475694"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PK144_16855"
     CDS             <1..1292
                     /locus_tag="PK144_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HNF67443.1"
                     /translation="PASVPASVPAHVPVLVIGGGQAGLSVSHYLGHAGIGHVVLERET
                     ALHAWRDQRWDHFTLVTPNWQCALPGHPYDGDDPHGFMNRGEILDYLERFAARVRAPL
                     REGVAVERLYLRDDGRFEAATSAGRCTADQVVVASGGYQQPVVPRCAERLPAGLVQLH
                     SAQYRNAAALPPGAVMVVGSGQSGAQIAEDLHLAGRQVHLATGDAPRCARFYRGRDVV
                     DWLADMGYYDLPVHEHPLREGVRDNTNHYVTGRDGGRDIDLRRFALEGMRLYGQALDL
                     HGGTMRFAPNLGAHLDAADATYNRINASIDEWIAKLGLVTAEPASTYQPLWTPPEEAA
                     SLDLAGAGITSVIWCIGYRADYGWIDAPVFNGRGYPAHVRGVTPQPGLYFIGLPWLHT
                     WGSGRFSGVGRDAEYLSARIGARLAQLAGAAPEALAG"
     gene            1289..2146
                     /locus_tag="PK144_16860"
     CDS             1289..2146
                     /locus_tag="PK144_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67444.1"
                     /translation="MTRLGMPQLAYAGLSETWLLRDCGDRHWLAIAARLGLADPDFRD
                     ADGRSAYAAFLGLRLTQARLDAAREHDRLDVQTRLSRPGPARHLSVHDLHIGPRRIAR
                     VELLSTFVGRLRPGDNRSVARVSVPADTTGDATDDAADNGQSDGDTGLPPLAQRVRAL
                     RAGVDLDAEGLLAPPATLRFSFTPCPRNDFNGAGFLYFANFAAVLDRAAWDWWREAPQ
                     QTTAVREIAWFGNLNVGETLHVGLLGVHRDGLGAGHACELRAGDGRLLARALTRRRPA
                     SASAPEPQP"
     gene            2143..>3511
                     /locus_tag="PK144_16865"
     CDS             2143..>3511
                     /locus_tag="PK144_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF67445.1"
                     /translation="MIRPTSPAARRSRRGLADLKPVYRAADVEGLAHLDGLPGEAPFV
                     RGPYPTMYRERPWTLRQYAGYRDAADSNACYRRLLADGGQGLSVAFDLPTHHGYDADD
                     PRIAADVGRAGVSVCCVGDMVRLFDGIPLDRVSVSMTMSAAVLPVLGAFVLAAEAQGV
                     PAQALRGTIQNDILKEFQARNAWIHAPGPSLRIALDVVEWLAAHAPHFNALSASGYHF
                     QETGADAVTELALTFANARACLRGLRARGHAVDAICRRFSFFFGAGLDFYVEIAKLRA
                     ARLVWAGIVEAEGGRDPAARALRMHCQTSGWSLAAGEPYNNLMRTTVEALAAVFGGTQ
                     SLHTNACDEALCLPGEATARLARNTQLILQRETGICDVADPWAGSYLIEHLTADIAAR
                     TRARLDAIEAAGGVLALTRDGTLAAGLREAGAALQASIDAGSLSRLGESGPAPFEPRA
                     FDGGHA"
BASE COUNT          439 a         1372 c         1216 g          484 t
ORIGIN      
        1 ttcctgcttc cgttcccgcg tccgttcccg cccacgtgcc ggtgctcgtc atcggcggcg
       61 ggcaggccgg actttcggtc agccactacc tcggccacgc cggcatcggt catgtcgtgc
      121 tcgaacgcga gaccgcgctg catgcctggc gcgaccagcg ctgggaccac ttcacgctgg
      181 tcacgccgaa ctggcagtgc gcgctgcccg gtcatccgta cgacggcgac gacccgcacg
      241 gcttcatgaa ccggggcgag atcctcgact acctcgaacg cttcgccgcg cgggtgcggg
      301 cgccgctgcg cgagggggtc gcggtcgagc gcctgtacct gcgcgacgac ggccgcttcg
      361 aggccgcgac cagcgccggc cgctgcacgg ccgatcaggt cgtcgtcgcc agcggcggct
      421 accagcagcc ggtcgtgccg cgctgcgccg aacgcctgcc cgccggcctc gtgcagctgc
      481 actcggcgca gtaccgcaac gccgcggcgc tgccgcccgg cgccgtgatg gtcgtcggct
      541 cgggccagtc cggtgcgcag atcgccgagg acctgcacct ggccggccgg caggtgcacc
      601 tcgccaccgg cgatgccccg cgctgcgcgc gcttctaccg cggccgggac gtggtcgact
      661 ggctcgccga catgggctac tacgacctgc cggtgcacga acacccgctg cgcgagggcg
      721 tgcgcgacaa caccaaccac tacgtgaccg gccgcgacgg cgggcgcgac atcgatctgc
      781 gccgcttcgc gctcgaaggc atgcgcctgt acggacaggc gctcgatctg cacggcggca
      841 cgatgcgctt cgcgccgaac ctcggcgcgc acctcgacgc ggccgatgcc acgtacaacc
      901 gcatcaacgc ctcgatcgac gaatggatcg cgaagctcgg cctcgtcacc gccgaaccgg
      961 cgagcacgta tcagccgctc tggacgccgc cggaggaagc cgcaagcctc gatctcgccg
     1021 gcgccggcat caccagcgtc atctggtgca tcggctaccg cgccgattac ggatggatcg
     1081 acgcgccggt gttcaacggc cgcggctacc cggcgcacgt gcgcggcgtc acgccgcagc
     1141 cgggcctgta cttcatcggc ctgccgtggc tgcacacctg gggttcgggc cgcttctcgg
     1201 gcgtcgggcg cgatgccgaa tacctcagtg cacgcatcgg tgcgcgcctc gcgcagctcg
     1261 ccggcgccgc gccggaggcg ctcgccggat gacgcgcctc ggcatgccgc agctcgcgta
     1321 cgcgggcctc tccgaaacct ggctgctgcg cgactgcggc gaccggcact ggctcgcgat
     1381 cgccgcccgg ctcgggctcg ccgatccgga cttccgcgac gccgacggcc gcagcgccta
     1441 tgccgctttc ctcggcctgc ggctgacgca ggcgcggctc gatgcggcgc gcgagcacga
     1501 ccgcctcgac gtgcagaccc ggctgtcacg gcccggtccg gcccggcacc tgagcgtcca
     1561 cgacctgcac atcggcccgc ggcgcatcgc gcgcgtcgag ctgctgtcga ccttcgtcgg
     1621 ccgcctgcgc cccggcgaca accgcagcgt cgcgcgcgtg tcggtgccgg ccgacacgac
     1681 cggcgacgcg accgacgatg ccgccgacaa tggccagtcc gacggcgaca ccggcctgcc
     1741 gccgctcgcg cagcgcgtgc gggcgctgcg cgcgggcgtc gacctcgatg ccgaaggtct
     1801 gctcgcgccg ccggcgacgc tgcggttttc gttcacgccg tgtccgcgca acgacttcaa
     1861 cggtgccggc ttcctgtact tcgcgaactt cgccgccgtg ctcgatcgcg ccgcctggga
     1921 ctggtggcgc gaagcgccgc agcagacgac ggcggtgcgc gagatcgcgt ggttcggcaa
     1981 cctcaacgtc ggcgaaaccc tgcacgtcgg gctgctcggc gtgcatcgcg acggcctcgg
     2041 cgccggccat gcctgcgagc tgcgcgccgg cgacggccgc ctgctcgcgc gggcgctgac
     2101 gcgccgccgc cctgcttccg cgtcagcacc ggagccgcag ccatgatccg tcccacctca
     2161 cccgccgccc gccgcagccg ccgcggactc gccgatctga aaccggtcta ccgcgccgcc
     2221 gatgtcgagg gcctcgcgca cctcgacggc ctgcccggcg aggcgccgtt cgtgcgcggt
     2281 ccctatccga cgatgtaccg cgagcggccg tggacgctgc gccagtacgc cggctaccgc
     2341 gacgccgccg acagcaacgc ctgctaccgc cgcctgctcg ccgacggcgg ccagggcctg
     2401 tcggtggcct tcgacctgcc gacccaccac ggctacgacg ccgacgatcc gcgcatcgcg
     2461 gccgatgtcg ggcgcgccgg cgtgtcggtg tgctgcgtcg gcgacatggt ccggctgttc
     2521 gacggcatcc cgctcgatcg cgtctcggtg tcgatgacga tgagcgccgc ggtgctgccg
     2581 gtgctcggcg ccttcgtgct cgcggccgag gcgcagggcg tgccggcgca ggcgctgcgc
     2641 ggcacgatcc agaacgacat cctcaaggag ttccaggcgc gcaatgcgtg gattcatgcg
     2701 cccggcccgt cgctgcgcat cgcgctcgat gtcgtcgaat ggctggccgc gcatgcgccg
     2761 cacttcaacg cgctgtcggc cagcggctac cacttccagg aaaccggcgc cgatgccgtc
     2821 accgaactgg cgctgacctt cgccaacgcc cgcgcctgcc tgcgcggcct gcgcgcgcgc
     2881 ggccacgccg tcgatgcaat ctgccggcgc ttcagcttct tcttcggcgc cggcctggat
     2941 ttctacgtcg agatcgcgaa gctgcgcgcc gcgcggctgg tctgggccgg catcgtcgag
     3001 gccgagggcg gacgcgatcc ggcggcgcgc gcgctgcgca tgcactgcca gaccagcggc
     3061 tggtcgctcg ccgccggcga gccgtacaac aacctgatgc gcacgaccgt cgaggcgctg
     3121 gccgcggtgt tcggcggcac gcagtcgctg cacaccaacg cctgcgacga ggcgctgtgc
     3181 ctgccgggcg aggccacggc gcggctggcg cgcaacacgc agctgatcct gcagcgcgag
     3241 accggcatct gcgacgtggc cgatccctgg gccggctcct acctgatcga gcacctgacc
     3301 gccgacatcg ccgcgcgcac gcgcgcccgg ctcgatgcga tcgaggctgc cggcggcgtg
     3361 ctcgcgctga cgcgcgacgg cacgctcgcc gccggcctgc gcgaggccgg cgccgcgctg
     3421 caggccagca tcgacgccgg ctcgctgagc cggctcggcg aatccggccc ggccccgttc
     3481 gagccgcgcg ccttcgatgg tgggcacgct g
//
LOCUS       DAOQIE010000368         3500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17085_length_3500_cov_7.746009, whole genome shotgun sequence.
ACCESSION   DAOQIE010000368 DAOQIE010000000
VERSION     DAOQIE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17085_length_3500_cov_7.746009"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PK144_16870"
     CDS             complement(<1..1075)
                     /locus_tag="PK144_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF67446.1"
                     /translation="MNMRSIRLHKALADAGLGSRRQIEEWIAAGRVTVNGTVAETGAS
                     IEGSENVCVDGRPVYLSSAPRRTRVLAYYKPDGELITRDDPEGRPTVFEHLPPLRDGR
                     WIAVGRLDLNTQGLLLLTSDGELANRLMHPSNGIERQYAVRVLGTVDADILQRLRDGV
                     LLEDGIARFDDIVDAGGSGANHWYQVILREGRNREVRRLWESQGVTVSRLMRVRYGPI
                     ELPRGLRAGRWAELDARQINDLRFAVGLPPQKDETPAFRGPRPGDSPRAGARKPFPKA
                     DARPGTGAGRGDKPWQGRKARSDDDTVGGYGTRPQGDSGRPRRPGERPASAGRPAQGE
                     RPWREGGAAARPAGDRPQGRACSR"
     gene            complement(1149..1709)
                     /gene="scpB"
                     /locus_tag="PK144_16875"
     CDS             complement(1149..1709)
                     /gene="scpB"
                     /locus_tag="PK144_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNF67447.1"
                     /translation="MQLKLILEAALLAAGEPLSPERLESLFTEAERPARAELRAALDA
                     LAQDLEARGIELVEVAGGWRLQVRSALAPWVSRLWEDKPGRYSRATLETLALIAWRQP
                     ITRGEIEAVRGVAVNSGIVRALLDRGWIRVLGHREVPGRPALYGTTREFLDHFGLKTL
                     ADLPALAELRDPAVIGAELHGAAGRD"
     gene            1904..2451
                     /locus_tag="PK144_16880"
                     /pseudo
     CDS             1904..2451
                     /locus_tag="PK144_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2717..>3500
                     /locus_tag="PK144_16885"
     CDS             2717..>3500
                     /locus_tag="PK144_16885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF67448.1"
                     /translation="MDIGFIGLGNMGLAMARNLVGAGHRLRAWNRSPIAPGALPGATL
                     VETPAEAFAAEVVFTMLADDAAIRAVLLDSGVLAGAAPGTVHVVSATISPTLAEELEA
                     LHLCGGLGYLSAPVFGRPDAAATAQLSILAAGDPATLAKVRPLFDVIGRRTFVLGDRP
                     AQANIAKLAGNMMIAMAIESMAEAVALTEAHGIDRATFLDLLLQTLFGCRVYETYGHK
                     IATGDYSAGFKMSLGLKDLRLATEAAGNVDAPLVMLNAVRARM"
BASE COUNT          485 a         1325 c         1183 g          507 t
ORIGIN      
        1 ggcggctgca cgcccggccc tgcggccgat cgccggccgg gcgtgcagcc gccccgcctt
       61 cgcgccacgg tcgctcgccc tgcgccggcc ggcccgcgga tgccgggcgc tcgcccgggc
      121 ggcgtgggcg gccgctgtcg ccctgcgggc gcgtgccgta accgccgacc gtgtcgtcgt
      181 cgctgcgcgc cttgcgaccc tgccagggct tgtcaccgcg gcccgcgccg gtgccggggc
      241 gcgcatccgc tttcgggaag ggcttgcgcg ccccggcgcg cgggctgtca cccgggcgcg
      301 gaccgcggaa ggccggggtc tcgtccttct gcggcggcag gccgacggca aagcgcagat
      361 cgttgatctg gcgcgcatcg agctcggccc agcgcccggc gcgcaggccg cgcggcagct
      421 cgatcggccc gtagcgcacg cgcatcaggc ggctgacggt cacgccctgc gattcccaga
      481 gccggcgcac ctcgcggttg cggccctcgc gcaggatcac ctgataccag tgattggcgc
      541 cgctgccgcc ggcatcgacg atgtcgtcga agcgcgcgat gccgtcctca agcagcacgc
      601 cgtcgcgcag gcgctgcagg atgtcggcat cgaccgtgcc gagcacgcgc accgcgtact
      661 ggcgctcgat gccgttcgac gggtgcatca ggcggttcgc gagttcaccg tcgctggtca
      721 gcagcagcag gccctgcgtg ttcaggtcga gccgcccgac cgcgatccag cgcccgtcgc
      781 gcagcggcgg caggtgctcg aacaccgtcg gccggccttc ggggtcatcg cgcgtgatca
      841 gttcgccgtc gggcttgtag taggcgagca cgcgcgtgcg ccgcggcgcg ctgctcaggt
      901 acaccggccg gccgtcgacg cagacgttct cgctgccttc gatgctcgcg ccggtttcgg
      961 cgaccgtgcc gttgacggtg acgcggccgg ccgcgatcca ttcctcgatc tggcgacgcg
     1021 agccgaggcc ggcgtcggcg agggccttgt gcaggcggat gctgcgcatg ttcatgtggg
     1081 gactccggga aaaatccgcg cggcgagcgc cggcgcggcg gggatgcgga cggattcggg
     1141 gcgccggctc agtcgcggcc ggccgccccg tgcagttcgg cgccgatgac ggccgggtcg
     1201 cgcagttcag cgagggccgg caggtcggcg agggtcttca gaccgaaatg atcgagaaat
     1261 tcgcgcgtcg tgccatacag cgccggccgg ccggggacct cgcggtgacc gagcacgcgg
     1321 atccagccgc gatcgagcag cgcacgcacg atgccgctgt tgacggcgac gccgcgcacg
     1381 gcctcgatct cgccgcgcgt gatcggctgg cgccaggcga tcagcgccag cgtctccagc
     1441 gtcgcgcgcg aataacgtcc gggcttgtcc tcccacaggc gcgacaccca cggcgccagc
     1501 gccgaacgca cctgcagccg ccagccaccg gcgacctcga ccagctcgat gccgcgcgct
     1561 tcgagatcct gcgccagcgc atcgagggcc gcccgcagtt cggcgcgcgc cggacgctcg
     1621 gcttcggtga acaggctctc cagccgttcc ggcgacagcg gctcgccggc cgcgagcagt
     1681 gcggcttcga ggatcagctt cagttgcatc ggacgaacgg gctccgggcg cgcgcgcaaa
     1741 gccggcggcg caggatacgc gcgtcccgtc ccgcacgcac ggctgatgcg gtgcatcacg
     1801 aaacccggca ccggcgccgc gcgtttgcta ttcccgtagc cgtatccgcc cggacgccgc
     1861 cctgatcgcg tccgcccgcc cgccacccga ggagtcgccc gcgatgccgg acacccccct
     1921 gctgccccgt cgcctgcatc acaatgcctg gaccacgcgc gacatggccg ccacccgtgc
     1981 cttctacgag gatgtgatcg gcctgccgct ggtcgcgacc tggtgcgaat ccgaccggct
     2041 gttcggcagg gtccgcacct atgtgcactg cttcttcgga cttgccgacg gcagcgcgct
     2101 ggcgttcttc cagttcgccg atgcgcccga tgccgaggaa ttcagccccc gatcccggaa
     2161 acgccgttcc atcacatcgc gctgaaggtc gacaagccgc tgcagcaggc gatcgagcag
     2221 cgcatcgcgg ccgccggcta cggcgaaccg cagaccttcg tgctcgatca cggctactgc
     2281 cgctcgctgt acatccgcga tccgaacggc ctgctgctcg aattcacgct cgacccgccc
     2341 ggggccgagc tgatcgccca cgaacgccag accgatgccc gtgccacgct ggagcgctgg
     2401 ctggccggcg accatgcttc gaacaactgc tatcgcggca acaccatctg atcctgcgca
     2461 tgcaaagcat gtgataccgg tcacaccgga gcactcggct gttcagctcc tcgccccctg
     2521 cgggagagcg tttggggaga ggagtggaaa aaccatgcac ttacgtctga ccggcatgat
     2581 ccggccgccc tttccgccgc gctgcccccg ggggagcagg accggcgcaa cgctggcagg
     2641 gacgcatccg ccctgagccg cctgcaacgg ccctcccgcc ctgacctgac attccaccct
     2701 gcgtacggag gcattcatgg acatcggttt catcggactc ggcaacatgg gcctcgccat
     2761 ggcccgcaac ctcgtcggcg ccggccaccg cctgcgcgcc tggaaccgct cgccgatcgc
     2821 gcccggcgcc ctgcccggcg cgacgctggt cgaaaccccg gccgaagcct tcgccgccga
     2881 ggtcgtgttc acgatgctcg ccgacgatgc cgcgatccgc gccgtgctgc tcgattccgg
     2941 cgtgctcgcc ggcgccgcgc ccggcaccgt gcatgtggta tcggcgacca tctcgccgac
     3001 gctcgccgag gaactcgaag ccctgcatct ctgcggcggc ctcggctatc tgtccgcccc
     3061 ggtgttcggc cgccccgatg ccgcggcgac cgcgcagctc agcatcctcg ccgccggcga
     3121 cccggccacg ctcgcgaagg tgcggccgct gttcgacgtg atcggccggc gcaccttcgt
     3181 gctcggcgat cgcccggcgc aggccaacat cgccaagctc gccggcaaca tgatgatcgc
     3241 gatggcgatc gagtcgatgg ccgaagccgt ggcgctgacc gaagcccacg gcatcgaccg
     3301 cgcgaccttc ctcgacctgc tgctgcagac gctgttcggc tgccgggtct acgagaccta
     3361 cggccacaag atcgccaccg gcgactactc ggccggcttc aaaatgagcc tcggcctcaa
     3421 ggacctgcgc ctcgccaccg aagccgcggg caatgtcgat gcgccgctgg tgatgctcaa
     3481 cgccgtgcgg gcacgcatga
//
LOCUS       DAOQIE010000369         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17178_length_3490_cov_6.584862, whole genome shotgun sequence.
ACCESSION   DAOQIE010000369 DAOQIE010000000
VERSION     DAOQIE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17178_length_3490_cov_6.584862"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PK144_16890"
     CDS             complement(<1..149)
                     /locus_tag="PK144_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNF67449.1"
                     /translation="MQRTEALTLLSHQRAVLRERFGVRRLALFGSTARNEARDSSDLD
                     LLVEF"
     gene            complement(193..1407)
                     /locus_tag="PK144_16895"
     CDS             complement(193..1407)
                     /locus_tag="PK144_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNF67450.1"
                     /translation="MTQTPADDRPAPGPLAGLRVLELGQLIAGPFTGTLLAYFGAEVI
                     KIEPPEGDPIRQWRVVRDGTSLWWHSIGRNKKSVRIDLKTGEGRALVGQLAARCDVLV
                     ENFRPGTLEKWGLGPEVLKAANPGLVIARISGYGQDGPYAGKAGYASVCEGFGGLRYV
                     NGMPGEPPVRPNLSIGDTLAALHAVIGILMALHQRGRSGSGQVVDVALFEAVYNLLES
                     VVPEFDGAGVVREPSGTTVTGIVPTNTYRCADGRWAIIGGNGDSIYKRLMRAAGRADL
                     ADDPRLAHNPGRVQHEAEIDAAIAAWTATLPLAEVLSSLETAGVPAGPIYSVRDMFAD
                     PQYQARGLFERVEIDGKPLAIPAIPPKLSATPGGTRWSGPAIGAHTGEVLGEWLGMDA
                     EAVNGLRQRGVI"
     gene            1744..3486
                     /gene="recJ"
                     /locus_tag="PK144_16900"
     CDS             1744..3486
                     /gene="recJ"
                     /locus_tag="PK144_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNF67451.1"
                     /translation="MPTAIVRRPLPSPGERPELPADLPPLLARVYALRGVASARELDQ
                     ALTALPAPQRLKGIDRAVDLLSAALSTDARILIVADYDADGATACALAVRGLRALGAR
                     HVDYLVPNRQRHGYGLTPAIVELAAARRPELLITVDNGIASLEGVAAAQARGWQVLVT
                     DHHLPGERLPAADAIVNPNQPGCSFPSKALAGVGVMFYVLLALRARLRTSGWYGACGL
                     TEPNLARLLDLVALGTVADVVPLDHLNRVLVGQGLARIRSGAAVPGIRALAEVAGRDP
                     ARLGCSDIGFTLAPRLNAAGRLEDMALGIECLLCDDPVRARELAVTLDRLNRERRDIE
                     AVMQAAALEDLERLGLDAAGLPFGLCLYDPHWHQGVVGILAARVRERTHRPTIVFAPG
                     DPGWLKGSARSVPGLHLRDALAAVDARHPGLIERFGGHAAAAGLTLAEANYPDFAAAF
                     DAEVRRHLGAADLRGVLYSDGELAPGDCTLAIAERLRDGGPWGQGFPEPLFDGEFEVV
                     AARTLKERHLKLSVRRPGLDEVLDAIAFGAAGSCPEPLPGTRLRLAYRLDVNEWNGRR
                     RLQLLVLHLEPAGA"
BASE COUNT          432 a         1218 c         1317 g          523 t
ORIGIN      
        1 tcgaattcga cgagcaggtc gagatcgctg ctgtcccggg cttcgttgcg ggcggtcgag
       61 ccgaacaggg ccaggcggcg cacgccgaaa cgttcgcgca gcaccgcgcg ctggtggctc
      121 agcagggtca gggcttcggt gcgttgcatg gcggacatcc tccgggtgag cgctggctgc
      181 cgccacggag cttcagatta ccccgcgctg ccgcagaccg ttcaccgcct ccgcatccat
      241 cccgagccat tcaccgagca cctcgccggt atgcgcaccg atcgccgggc ccgaccagcg
      301 cgtgccgccg ggggtggccg acagcttcgg cgggatggcc gggatcgcca gcggcttgcc
      361 gtcgatctcg acgcgctcga acaggccgcg ggcctggtac tgcggatcgg cgaacatgtc
      421 gcggacgctg tagatcgggc cggccggcac gccggcggtt tcgaggctcg acaggacttc
      481 ggcgagcggc agcgtggcgg tccatgcggc gatcgcggcg tcgatctcgg cttcgtgctg
      541 cacgcggccg gggttgtgcg cgaggcgcgg atcgtcggcc aggtcggcac ggccggcggc
      601 gcgcatcagg cgcttgtaga tggaatcgcc gttgccgccg atgatcgccc agcggccgtc
      661 ggcgcagcgg taggtgttgg tcggcacgat gccggtcacc gtcgtgcccg agggctcgcg
      721 tacgacgccg gcgccgtcga actcggggac cacgctttcg agcaggttgt agacggcttc
      781 gaacagcgcc acgtcgacga cctgaccgct gccggagcgc ccgcgctggt gcagcgccat
      841 caggatgccg atcaccgcgt gcagcgccgc gagcgtgtcg ccgatcgaca ggttcgggcg
      901 caccggcggt tctccgggca tgccgttgac gtagcgcagg ccgccgaagc cctcgcagac
      961 cgaggcgtag ccggccttgc cggcgtaggg gccgtcctgg ccgtagccgg agatgcgcgc
     1021 gatcacgagg ccggggttcg cggccttcag cacctccgga cccaggcccc atttctccag
     1081 cgtgccgggg cggaagttct cgacgagcac gtcgcagcgc gcggcgagct ggccgaccag
     1141 cgcccggccc tcgccggtct tcaggtcgat gcggacgctc ttcttgttgc gaccgatgct
     1201 gtgccaccac agcgaggtgc cgtcgcgcac gacgcgccac tggcggatcg ggtcgccttc
     1261 gggcggttcg atcttgatga cctcggcgcc gaagtacgcg agcagcgtgc cggtgaacgg
     1321 gccggcgatg agctggccga gttcgagcac gcgcagcccg gcaagcgggc cgggggccgg
     1381 acggtcatcg gcaggagtct gggtcatggc ggggcttccg ggcgcgggct gcgggacggg
     1441 tggatggccg gggagtgtgg cgacggccgc cggcgtctgc cagtcgcagg ctgcaggctt
     1501 tggcagcgcg ctgcgcgcag ccgcaacggt gaccggtgcc ggagcctgcc gatgtgctcc
     1561 cggtgtcgca ggtcttgaat gaccatggct ggttcgtggt tgaattcgca gttttggagc
     1621 ggtgcagcca ttaccttggg ggcactgcgg cgcaagcgtg ccaggcgccg gctccgtccc
     1681 ccttgccgcg cccgcagcat ccggcgatgc tgcgccgcgc ccgccgcgat gagcccgttc
     1741 tgaatgccga cagcaatcgt acgccgaccc ctgccgagcc ccggcgaacg gccggaactg
     1801 cccgccgacc tgccgccgct gctggcgcgg gtctatgcgc tgcgcggggt tgccagcgcc
     1861 cgtgagctcg accaggcgct gacggcgctg ccggcaccgc aacggctgaa gggcatcgat
     1921 cgcgccgtcg acctgctgag cgcggcgctg agcaccgatg cccgcatcct gatcgtcgcc
     1981 gactacgacg ccgatggcgc caccgcctgc gcgctggcgg tgcgcgggct gcgcgcgctc
     2041 ggggcgcgcc acgtcgatta tctggtgccg aaccgccagc gccacggtta cgggctgacg
     2101 ccggcgatcg tcgaactcgc ggccgcacgg cggccggagc tgctgatcac cgtcgacaac
     2161 ggcatcgcca gcctcgaagg cgtcgccgcg gcgcaggcgc gcggctggca ggtgctggtc
     2221 accgatcacc acctgcccgg cgagcggctg ccggcggccg atgccatcgt caatccgaac
     2281 cagccgggct gcagctttcc gtcgaaggcg ctggccggcg tcggcgtgat gttctacgtg
     2341 ctgctggcgc tgcgcgcgcg gctgcgtacg agcggctggt acggcgcctg cgggctgacc
     2401 gagccgaacc tcgcgcgcct gctcgatctg gtcgcgctcg gcaccgtcgc cgatgtcgtg
     2461 ccgctcgatc acctcaaccg cgtgctggtc gggcagggcc tggcccgcat ccgcagcggc
     2521 gccgccgtgc ccggcatccg cgcgctggcc gaggtcgccg gccgcgatcc ggcgcgcctc
     2581 ggctgttccg acatcggctt cacgctggcg ccgcgcctga acgccgccgg ccggctcgaa
     2641 gacatggcgc tcggcatcga atgcctgctc tgtgacgatc cggtgcgcgc gcgcgagctg
     2701 gccgtgacgc tggaccgcct gaaccgcgag cggcgcgata tcgaggccgt gatgcaggca
     2761 gcggcgctgg aggacctcga acggctcggg ctcgatgcgg ccgggctgcc gttcggtctg
     2821 tgcctgtacg acccgcactg gcatcagggc gtggtcggca tcctcgcggc gcgcgtgcgc
     2881 gagcgcacgc accggccgac gattgtcttt gcgcccggcg atccgggctg gctgaagggt
     2941 tcggcgcgct cggtgccggg cctgcacctg cgcgatgcgc tggccgcggt cgatgcgcgc
     3001 catccggggc tgatcgagcg cttcggcggt cacgccgccg ccgccggcct gacgctggcc
     3061 gaggcgaatt atccggactt cgctgcggct ttcgatgccg aggtacgccg tcacctcggt
     3121 gccgcggacc tgcgcggcgt gctgtattcc gacggggagc tggcgccggg cgactgcacg
     3181 ctcgcgatcg ccgagcgcct gcgcgacggc gggccgtggg gtcagggctt tccggagccg
     3241 ctgttcgacg gcgagttcga ggtcgtcgcc gcgcgcacgc tgaaggagcg gcacctgaag
     3301 ctcagcgtgc gccggccggg gctggacgag gtgctcgatg cgatcgcctt cggagcggcc
     3361 gggagctgcc cggaaccgct gccgggcacg cggctgcgcc ttgcgtaccg gctggacgtg
     3421 aacgaatgga acgggcgccg gcggctgcag ctgctggtgc tgcatctgga gccggccggt
     3481 gcctgagcgg
//
LOCUS       DAOQIE010000370         3487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17201_length_3487_cov_7.398601, whole genome shotgun sequence.
ACCESSION   DAOQIE010000370 DAOQIE010000000
VERSION     DAOQIE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17201_length_3487_cov_7.398601"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PK144_16905"
     CDS             complement(<1..562)
                     /locus_tag="PK144_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNF67452.1"
                     /translation="MEFKDYYQILGVSRTAAEDEIKKAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLRDPEKRADYDRLGPNFRNGQDFRPPPGWRPHAGAGGAHRGGGPSAA
                     DGFSQADFSEFFESIFGGGGGGAQGFGARAHGARGGQQRRPAARDGEDRTLALEITLE
                     EAFAGGSRQVEISGASGRRTLNVRIPA"
     gene            complement(680..1126)
                     /locus_tag="PK144_16910"
     CDS             complement(680..1126)
                     /locus_tag="PK144_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNF67453.1"
                     /translation="MSSNRYDPWTRLGLLTKELERVFDPRLANNEDASNVATCDWVPA
                     VDIKEEAERFLIHADIPGVDPKDIDITMENGVLTLRGQRSSERREAADGWKRVERTHG
                     SFYRRFGLPDTADASRISARGQNGVLEIVIPKQERAAPRKITVSAE"
     gene            1339..1860
                     /gene="moaB"
                     /locus_tag="PK144_16915"
     CDS             1339..1860
                     /gene="moaB"
                     /locus_tag="PK144_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNF67454.1"
                     /translation="MSEARQFQPLSIAVLTVSDTRGEAEDVSGRTLVERLEAAGHRLA
                     EKAMCRDDLYAIRAIVSRWIADPAVQVVISTGGTGVTGRDGTPEAVQVLLDKEISGFG
                     ELFRMLSWEEIRTSTIQSRALAGVANGTYLFVLPGSSGACRTAWDRILKDQLDYRHRP
                     CNLVELMPRLLEK"
     gene            1886..2908
                     /locus_tag="PK144_16920"
     CDS             1886..2908
                     /locus_tag="PK144_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008927622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF67455.1"
                     /translation="MNEALDGPLNEAVNLPAVTAGPAKLVRRAPGEPIVGLALGSGSA
                     RGWAHIGVLRALAALGIEPQIVTGCSVGALVAAAWASGRLDALEAWVRRLDWWQMLNF
                     VDLGPGALIAGERLRTACIEHIGEHDIETLPVRFGCVATDLARGREVWFQSGSLLDAV
                     RASMALPGVFPPVRGEDGAWLVDGGLVDPVPVSLARALGAERVIAVNLNGDIVGRHLV
                     RRRRSPAEVLIEPVAARLSALFGREREAPPAPDSTLPVPLEDAQPGLFEVLFSAMNIM
                     QDRITRSRMAGDPPDMLLAPRLAHLALVEFDRAAEAIEVGRIAVEQHRAALLAAVGRP
                     LPAGEP"
     gene            2914..>3487
                     /locus_tag="PK144_16925"
     CDS             2914..>3487
                     /locus_tag="PK144_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF67456.1"
                     /translation="MDANLAQYAAAAVVGLLGGVHCVGMCGGIVGVLTLGLPAETRAR
                     AARLLPYLVAYNAGRLASYTLIGALAGGVGAYAAHLVSVRHAQLGLQLIAGGFMVVLG
                     LYLAGVWAGITRIERAGGGLWRRIEPLGRRLLPVRTPAQALGCGLVWGWLPCGLVYSV
                     VVWAIAAGSAAEGARLLLAFGLGTLPALFAM"
BASE COUNT          408 a         1271 c         1261 g          547 t
ORIGIN      
        1 cggccgggat gcgcacgttc agcgtgcgcc ggccgctagc gccgctgatc tcgacctggc
       61 gcgagccgcc ggcgaaggct tcctccagcg tgatctccag cgccagcgtg cggtcctcgc
      121 cgtcgcgcgc cgccggccgg cgctgctggc cgccgcgcgc cccgtgggcg cgggcaccga
      181 agccctgcgc accgccgccg ccgccgccga agatgctctc gaagaactcg ctgaagtcgg
      241 cctgcgagaa gccgtcggcc gcgctcggcc cgccgccccg gtgcgcacca ccggcaccgg
      301 cgtgcggacg ccagcccggc ggcggacgga agtcctggcc gttgcggaag ttcggcccga
      361 gccggtcgta gtccgcgcgc ttttcgggat cgcgcaggac ttcccaggct tcggcgacct
      421 ccttgaagcg cgcctcggca tcgggctcct tgctgacgtc cgggtggtac ttgcgcgcga
      481 gccgccggta ggccttcttg atctcgtcct cggccgccgt gcgcgacacg ccgaggatct
      541 ggtagtagtc cttgaattcc ataccgttta ccgcactgcg ggccgggccg ctgaagcgac
      601 ccggcccggc gtggagactc ggggacacgc cccccgccgt gcgggcgggg ggcgcgggct
      661 gcgcgatgcg ctcagggcgt cattcggcgc tgaccgtgat cttgcgcggt gccgcgcgtt
      721 cctgcttcgg aatcacgatc tccagcacgc cgttctggcc gcgggcgctg atgcgcgagg
      781 catcggcggt gtccggcagg ccgaagcggc ggtagaagct gccgtgcgtg cgctcgacgc
      841 gcttccagcc atcggccgcc tcgcgccgct cggagctgcg ctggcccctg agcgtcagca
      901 cgccgttttc catcgtgatg tcgatgtcct tcggatcgac accggggatg tcggcatgga
      961 tcaggaagcg ctcggcttcc tccttgatgt cgacggccgg cacccagtcg caggtcgcga
     1021 cgttggaggc atcctcgttg ttggccagac gcggatcgaa cacgcgctcc agctccttgg
     1081 tcagcaggcc cagacgggtc cacgggtcgt agcggttgct gctgatgctc atgttgcacc
     1141 tcggaaaatc gctgcgttgt gtcgggggga agcggtcatc gcgaccgccg tgatccgtta
     1201 gataaggtcg ggccggaggc tttcaagagc ggcggattca ggccggcatg cgccggatca
     1261 tcaccggcgc actggccgcc ggcggtccga tcgggtatct agccgcgact gcccctgccc
     1321 ccgccgagga cctcgccgat gagcgaagcg cgccagttcc agcccctgtc gatcgccgtg
     1381 ctgaccgttt ccgacacgcg cggcgaagcc gaggatgtct ccggccgcac gctggtcgag
     1441 cgcctcgaag ccgccggtca ccggctcgcc gaaaaggcga tgtgccgcga tgacctctat
     1501 gcgatccgcg ccatcgtctc gcgctggatc gccgatccgg ccgtgcaggt cgtcatcagc
     1561 accggcggca ccggcgtcac cggccgcgac ggcacgcccg aggccgtgca ggtgctgctc
     1621 gacaaggaaa tctccggctt cggcgagctg ttccgcatgc tgtcctggga ggagatccgc
     1681 acctcgacca tccagtcgcg cgcgctcgcc ggggtcgcca acggcaccta cctgttcgtg
     1741 ctgcccggct ccagcggcgc ctgccgcacg gcctgggacc gcatcctcaa ggatcagctc
     1801 gactaccggc accggccgtg caacctcgtc gagctgatgc cgcggctgct ggaaaagtga
     1861 gcgcaccgag cggcgcccgt catccgtgaa cgaagccttg gacggacccc tgaacgaagc
     1921 ggtgaacctg ccggccgtca ccgccggccc ggcgaagctc gtgcgccgcg cccccggcga
     1981 gccgatcgtc gggctcgcgc tcggcagcgg ttcggcgcgc ggctgggcgc acatcggcgt
     2041 gctgcgtgcg ctggccgcgc tcggcatcga gccgcagatc gtcaccggct gctcggtcgg
     2101 tgcgctcgtg gccgccgcct gggcctcggg ccggctcgat gcgctcgaag cctgggtgcg
     2161 acggctcgac tggtggcaga tgctgaactt cgtcgatctc ggccccgggg cgctgatcgc
     2221 cggcgaacgc ctgcgcacgg cctgcatcga gcacatcggc gagcacgaca tcgagaccct
     2281 gccggtacgc ttcggctgcg tggcgaccga tctggcgcgc gggcgcgagg tctggttcca
     2341 gtccggcagc ctgcttgatg ccgtgcgcgc gtcgatggcg ctgcccggcg tgttcccgcc
     2401 ggtgcgcggc gaggacggcg cctggctggt cgacggcggg ctggtcgatc cggtgccggt
     2461 gtcgctcgcg cgtgcgctcg gggccgagcg cgtgatcgct gtcaacctca acggcgacat
     2521 cgtcggccgc cacctggtgc gccgccggcg cagcccggcc gaagtgctga tcgagccggt
     2581 cgccgcgcgg ctgtcggcgc tgttcggccg cgagcgcgag gcgccgccgg ccccggactc
     2641 cacgctgccg gtgccgctcg aggacgccca gcccggcctg ttcgaggtct tgttcagcgc
     2701 gatgaacatc atgcaggacc gcatcacccg ctcgcgcatg gccggcgatc cgccggacat
     2761 gctgctcgcg ccgcggctcg cgcatctggc gctggtcgag ttcgaccgtg ccgcggaagc
     2821 catcgaggtc ggccgcatcg ccgtcgaaca gcaccgcgcc gcgctgctgg ccgcggtcgg
     2881 ccggccgctg ccggccgggg agccctgatc accatggatg cgaacctcgc gcagtacgcg
     2941 gctgcggccg tggtcggcct gctcggtggc gtgcactgcg tcggcatgtg cggcggcatc
     3001 gtcggcgtgc tgacgctcgg cctgccggcc gaaacccgcg cgcgcgcggc gcggctgctg
     3061 ccgtatctcg tggcctacaa cgccgggcgg ctggcgagct acacgctgat cggcgccctc
     3121 gccggcggtg tcggcgccta cgcggcgcat ctcgtctcgg tgcgccacgc ccagctcggc
     3181 ctgcagctca tcgccggcgg cttcatggtc gtgctcggtc tgtatctggc cggcgtctgg
     3241 gccggcatca cgcgcatcga acgcgccggc ggcgggctgt ggcggcgcat cgaaccgctc
     3301 ggccgccggc tgctgccggt gcgcacgccg gcgcaggcgc tcggctgcgg gctggtctgg
     3361 ggctggctgc cgtgcgggct ggtctacagc gtcgtggtct gggcgatcgc cgccggcagc
     3421 gccgccgaag gcgcccggct gctgctcgcc ttcggcctcg gcacgctgcc ggcgctgttc
     3481 gcgatgg
//
LOCUS       DAOQIE010000371         3480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17257_length_3480_cov_6.863650, whole genome shotgun sequence.
ACCESSION   DAOQIE010000371 DAOQIE010000000
VERSION     DAOQIE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17257_length_3480_cov_6.863650"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1982)
                     /locus_tag="PK144_16930"
     CDS             complement(477..1982)
                     /locus_tag="PK144_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNF67457.1"
                     /translation="MELIGHLQLGFAAAAGAQNLLACFTGALLGTLIGVLPGLGPVAT
                     IALLLPLTYGLPPLTALIMLAGIYYGAQYGGSTTSILLNLPGEASSVVTCRDGHPLAK
                     QGRAGTALAVAALGSFFAGCVGTLFLAACAPLLMRFALDFGPAEYCSLMLFGLSGAVA
                     IASRSLLKSLAMVVLGLLLGLIGTDANSGVARYTFGLRELDDGVGTTVLAVGVFGFAE
                     LLHGLEHACRRTPAMRPCGRLWPDRADTRRAVPAVLRGTALGTLLGLLPGAGALLPAF
                     ASYALEKACARDPARFGQGAIEGVAGPESANNAAAQTAFIPMLTLGIPSNAIMALMIG
                     ALTLHDIQPGPQVIVHDPVLFWGLVASMWIGNAMLLVLNLPLIGLWVRLLATPYRLLY
                     PAILLFCCLGLYALANRTGDLYLAALFGLLGYLLQKSGCETVPLLLGFVLGPLFEEHL
                     RRALLLARGDASVFLQRPLSLALLLAALALILGALSPTARRARRQLATEPG"
     gene            complement(1973..2428)
                     /locus_tag="PK144_16935"
     CDS             complement(1973..2428)
                     /locus_tag="PK144_16935"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNF67458.1"
                     /translation="MFHDPRDGLVGLALAATGAGALWIARRYPAGTAADLGPGFLPGI
                     LGALLLLLGLLMAAGACRGPRTPFPVLQLRPLLAVLGGVVLFGLLLRSAGLLVASSAL
                     VLLASAGLRGAGWRVALIEAVVLGVLAVAVFVHGLGLRLPVWPAGLPWN"
     gene            complement(2464..3441)
                     /locus_tag="PK144_16940"
     CDS             complement(2464..3441)
                     /locus_tag="PK144_16940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNF67459.1"
                     /translation="MFRRLTALSMMLMMLTTLVPVPAGAQAGEYPQRPVTLIVTLPAG
                     GPTDAAARTLATALSQALGQPFVVENRPGADGAIGARAVVEAAPDGYTLLFSVGSLVA
                     LPHLQSPPPFDALRDLAPVAAIGSYPFALFVHPSVPAANVAEFIEYARSRPEPLFYSA
                     STPGEALAATAFMQATGVPLTRVPYKGSSQALPDLLAGRVQLTFGPVATGLPHVREGL
                     LNALAVLTPKRLPALPDTATLEEAGIRGVTVPSWQAMLAPAGTPPAILDQLASATAAA
                     LETAPVRERLTKLLLQIELLPPAALAQRIEADDALWQRHFAATAAARQP"
BASE COUNT          584 a         1207 c         1267 g          422 t
ORIGIN      
        1 agggggccga tggcgcgcag ggtcgcggca aaagatggtt gaccctgaac atcggagccc
       61 tctgcatccg gattgatcca tgccgattcg ctatcggcag cttgctggtg ccctccgtga
      121 ccaagcggtc ggcctgtcgt gatttccctg caccccggtg accagtgagg ccaacttaca
      181 cccatgagac ataagttgta ttaaatacaa aaaaatagtg gatatttgat gctgtatcgc
      241 agcactgctc ctcggaattc tggtcatgct tcatgccgtt ctcaggattt ttcatacatg
      301 tgagttactt gatctcttgt gcgtcagatt tttctgccct gactgcgact tgactgactc
      361 ggaagccggt tgctcaggca ggcataagcc gggagttcgc cccgcgtccg gggaaggatg
      421 aaaggtgatc ttcagatcag gggcttgcga agcctggcag cattttctga gtgccctcag
      481 cccggttcgg tggccagttg ccggcgggcg cggcgggcgg tcgggctcag cgcgccgagg
      541 atcagcgcca gtgccgccag cagcagcgcg agcgacagcg gccgctgcag gaacacgctc
      601 gcgtcgccgc gcgcgagcag cagcgcgcgg cgcaggtgct cctcgaacag cgggccgagc
      661 acgaagccga gcagcagcgg cacggtctcg cagccggatt tctgcagcag atagccgagc
      721 agcccgaaca gcgcggccag gtacagatcg cccgtgcggt tggccagcgc gtacaggccg
      781 aggcagcaga acagcaggat cgccggatac agcagccggt acggggtggc cagcagccgt
      841 acccacagcc cgatcagcgg caggttcagc accagcagca tcgcgttgcc gatccacatg
      901 ctcgcgacca gcccccagaa cagcaccgga tcgtggacga tgacctgcgg acccggctgg
      961 atgtcgtgca gcgtcagcgc accgatcatc agcgccatga tggcgttcga cgggatgccg
     1021 agcgtcagca tcgggatgaa ggcggtctgc gcggcggcgt tgttcgccga ttccggtccg
     1081 gcgacgccct cgatcgcgcc ctggccgaag cgggcagggt cgcgcgcgca ggccttttcg
     1141 agcgcgtagg acgcgaaggc cggcagcagc gccccggcgc ccggcagcag cccgagcagc
     1201 gtgccgagtg cggtgccgcg cagcaccgcc ggcacggcac gccgggtgtc ggcccgatcc
     1261 ggccacagcc ggccgcaggg gcgcatcgcc ggcgtgcgcc ggcaggcgtg ctccagcccg
     1321 tgcagcaact cggcgaagcc gaacacgccg acggcgagca cggtcgtgcc gacgccgtca
     1381 tcgagttcgc gcagcccgaa ggtgtagcgc gcgacgccgg agttcgcgtc ggtgccgatc
     1441 agcccgagca gcagcccgag cacgaccatg gccagcgact tcagcagcga gcggctggcg
     1501 atcgcgacgg cgccgctcag gccgaacagc atcagcgaac agtattcggc cggtccgaag
     1561 tcgagcgcga agcgcatcag cagcggcgcg caggcggcaa ggaacagcgt gccgacgcag
     1621 ccggcgaaga aggagccgag cgcggcgacc gccagtgccg tcccggcccg gccctgcttc
     1681 gccagcggat gaccgtcccg gcaggtgacg accgagctgg cctcgccggg caggttgagc
     1741 aggatcgacg tggtcgagcc gccgtactgc gcgccgtaat agatgccggc cagcatgatc
     1801 agcgccgtca gcggcggcag gccgtaggtc agcggcagca gcagcgcgat ggtcgcgacc
     1861 gggccgaggc ccggcagcac gccgatcagc gtgccgagca aagcaccggt gaagcaggcg
     1921 agcaggttct gcgccccggc ggcggcggcg aagccgagct gcaggtgccc gatcagttcc
     1981 acggcagccc cgcaggccag accggcagcc gcaggccgag cccgtgcacg aacacggcga
     2041 cggccagcac cccgagcacg acggcctcga tcagcgccac ccgccagccg gcgccgcgca
     2101 ggccggcgct cgcgagcagc accagcgcgc tgctcgccac cagcagcccg gcactgcgca
     2161 gcagcagccc gaacagcacg acgccgccga gcacggcgag cagcggccgc agctgcagca
     2221 ccgggaacgg agtgcgcggg ccgcggcagg cgccggcggc catcagcagg ccgagcagca
     2281 gcagcaaggc cccgagtatg cccggcagga agccgggtcc gaggtcggcg gccgtgccgg
     2341 ccggatagcg ccgggcgatc cacagcgccc cggcgccggt ggcggcgagg gccagtccga
     2401 ccagtccgtc gcgagggtca tgaaacacgg gaaaggggct ccgcgggccg ccggggcgtc
     2461 cgctcacggc tggcgggccg cggcggtggc ggcgaaatga cgctgccaga gcgcgtcatc
     2521 ggcctcgatg cgctgcgcca gcgccgccgg cggcagcagc tcgatctgca gcagcagctt
     2581 cgtcaggcgc tcgcgcaccg gggcggtttc cagcgccgcg gcagtcgccg aggccagctg
     2641 atcgaggatc gccggcggcg tgccggccgg ggccagcatc gcctgccacg aaggcaccgt
     2701 cacgccccgg atgccggctt cctccagcgt cgcggtgtcg ggcagcgccg gcaggcgctt
     2761 cggcgtcagc accgcgagcg cgttcagcag cccctcgcgc acgtgtggca ggccggtggc
     2821 caccgggccg aaggtcagct gcacgcggcc ggccagcagg tcgggcaggg cttgcgacga
     2881 gcccttgtac ggcacgcgcg tcagcggcac gccggtggcc tgcatgaagg cggtcgcggc
     2941 cagcgcctcg ccgggcgtgc tcgcggagta gaacagcggt tcgggacggc tgcgcgcgta
     3001 ttcgatgaat tccgcgacgt tcgccgccgg caccgaggga tgcacgaaca gtgcgaacgg
     3061 atagctgccg atcgcggcga ccggcgccag atcgcgcagg gcatcgaagg gcggcggcga
     3121 ctgcaggtgc ggcagcgcga ccagcgagcc gacgctgaac agcagcgtgt agccgtcggg
     3181 ggccgcctcg acgaccgcgc gcgcgccgat cgcgccatcg gcgcccgggc ggttctcgac
     3241 gacgaagggc tggccgagcg cctgcgacag cgccgtcgcc agcgtgcgcg ccgcggcatc
     3301 ggtcggtccg ccggccggca gcgtcacgat cagcgtcacc ggccgctgcg ggtactcgcc
     3361 cgcctgcgcg ccggcgggca ccggcacgag cgtcgtcaac atcatcagca tcatcgacaa
     3421 ggctgtcagc ctgcggaaca tggcgcctcc cgtggaatgg tcgtcagggc gggcaccggc
//
LOCUS       DAOQIE010000372         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17284_length_3477_cov_5.744594, whole genome shotgun sequence.
ACCESSION   DAOQIE010000372 DAOQIE010000000
VERSION     DAOQIE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17284_length_3477_cov_5.744594"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2380
                     /locus_tag="PK144_16945"
     CDS             <1..2380
                     /locus_tag="PK144_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNF67460.1"
                     /translation="GLFLSSELQYARGAVRDLLALGQWSDAEFAATALAPLFTAGSND
                     GGAMAAAATVPVMEPLALGEDQLDAVRDGLNAPLTVVTGPPGTGKSQVAVAVLASAAL
                     SGRSVLFASRNHQAIEAVAGRLTELLAHQPLLIRANARDGGEGISFARAIDAILARPG
                     AEARREQLAESITVLSRLDAARMRALDTATAAALAAEELGRLEATIADREAALGIAQG
                     ADLPSVPIPMPAPTPRGWLMRGFAPWLRRLRLRRLRRIALDWQRLGLAAPDESTLELH
                     EQRLAALTELAELRRQRAALEASVRAGTGAADLEDPVRLGERMQASALQQLQTLAGVL
                     AECAPTDRQALTALRGDVVLANHDGTAGAARLRERWAAQRTLILGHMPLWAVSNLGAA
                     SRIPLVPGLFDYVVIDEAAQCDIASALPLLARARRAIVIGDPAQLPVITQVPVAWEVQ
                     ALRAAGRFTPGIGSYLYSANSLFHLAAAADTAKHHLLRDHYRCHEDIADYISATFYGR
                     RLRPLTDPQRLQAPAGQRAGFHWTPVHGPLLPARTGCAAPAEIDAIAAELQRLLVDEG
                     YAGSVGVVTPFREQANRLRDRLERSLPAGTIARARLEVHTAHGFQGDARDLMLFSLCA
                     GADLPAGARAFLRETGHLVNVAVSRARAVCHVFGDLDYAARCGIPHLEALLARRRTAP
                     AGPAGFESPWEERLHRALAARGVQTIAQYPVAGRRLDLALLSDAVKLDIEVDGDRYHR
                     DPDGRRKAADLWRDHQLRALGWRVVRFWVYELRENMDECVERVIAGTRPAGP"
     gene            2331..>3477
                     /locus_tag="PK144_16950"
     CDS             2331..>3477
                     /locus_tag="PK144_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67461.1"
                     /translation="MSASNASSPAPDRPGHEPIPPSAAPPAWPRVQLALGLASALALG
                     SFCSVLFGVVGDASERRNWQAQRVGHLDALGTLGTERSRLERELAELGRNREALAGDL
                     ARLRTETESGQAAATRLREEAGKLRQEVTDNRAELEAAGRRRDGLASAIRNAEATLTG
                     LNDQRETAERQSGEAEERRRAAAEAERSARAQQGKAEQGRDAALAQQKEAERRLGQAE
                     QELKTAAERVDGLHGQETELKRTVTTAGAERDRLKDEAKRLEQELARLDQQRQQLERD
                     TRGAADAQRAAEAARQQQERVNRDLQTAQAALSNAQGQLGETRGQQTQLAEEVARLRR
                     QKTELDGTVSAAAEAQRAADGARQQLERLNRDLQTAQAALSNAQGQLG"
BASE COUNT          536 a         1297 c         1219 g          425 t
ORIGIN      
        1 gggcctgttc ctgtcgagcg agctgcagta tgcccgcggc gcggtacgcg atctgcttgc
       61 actcggccag tggtcggatg ccgaattcgc cgcaacggcg ctggccccgc tgttcacagc
      121 tggctcaaac gacggcggcg cgatggccgc agccgccacc gtaccggtca tggagccgct
      181 cgcgcttggc gaggaccagc ttgatgcggt gcgggacgga ctcaacgcgc cgctgaccgt
      241 cgtgaccggc ccgcccggca ccggcaagtc gcaggtcgca gtcgccgtgc tggccagcgc
      301 cgcgctgagc gggcgcagcg tcctgttcgc cagtcgcaac catcaggcca tcgaagccgt
      361 cgccggacgg ctcaccgaac tgcttgcaca tcagccgctg ctgatccgtg ccaacgcccg
      421 cgacggtggc gaaggcatca gcttcgcgcg tgcgatcgac gcgattctcg cccgccccgg
      481 cgccgaagcc cggcgcgagc agctggccga atcgataacc gtgctgtccc ggctcgatgc
      541 cgcgcgcatg cgcgccctcg acacggcgac ggctgcggca ctggccgccg aagaactcgg
      601 ccggctggaa gccacgatcg ccgaccggga ggctgcactc ggcattgcgc agggggcaga
      661 cctgccatcg gtgcccatcc cgatgccggc gccgacgccg cgtggctggc tgatgcgcgg
      721 cttcgcccca tggctgcggc gcctgcggct gcggcggctg cggcgcattg cgctcgactg
      781 gcagcggctc ggcctggctg cgccggacga atcgacgctg gaactgcacg aacagcgact
      841 cgccgcactc accgaactgg ccgagctgcg cagacagcgt gccgccctgg aagccagcgt
      901 gcgggcaggc accggtgctg ccgacctcga agacccggtc aggctcggcg aacggatgca
      961 ggcctcggcg ctgcagcagc tgcagaccct cgccggcgtg ctcgccgaat gcgctccgac
     1021 cgaccgtcag gcgctgacgg ccctgcgcgg cgatgtcgtg cttgcaaacc acgacgggac
     1081 ggccggggcg gcacgcctgc gcgagcgctg ggccgcgcag cgcacgctga tcctcggcca
     1141 tatgccgtta tgggcggtat cgaacctcgg ggctgccagc cgcatcccgc tggtgccggg
     1201 gctgttcgat tacgtcgtga tcgacgaagc cgcccagtgc gacatcgcct cggccctgcc
     1261 gctgctcgcg cgcgcccggc gcgccatcgt gatcggcgat ccggcgcagc tgccggtcat
     1321 cacgcaggtg ccggttgcct gggaagtgca ggccctgcgg gctgccgggc ggttcacacc
     1381 cggcatcggc agctacctgt attcggccaa ctccctgttc cacctcgctg cggcggcgga
     1441 cacggcgaag caccacctgc tgcgcgacca ctaccgctgc cacgaagaca tcgccgacta
     1501 catcagcgcc acgttctacg gccggcggct gcgcccgctg accgatccgc agcggctgca
     1561 ggcaccggct ggacagcggg ccggcttcca ctggacgccg gtacacggac cgctgctgcc
     1621 ggcacgcacc ggctgtgcgg cgccggccga gatcgacgcc atcgcagccg aactgcagcg
     1681 gctgctggtg gatgagggct atgccggcag cgtcggcgtc gtcacgccgt ttcgcgagca
     1741 ggccaaccgc ctgcgcgacc ggctcgaacg cagcctgccg gccgggacca tcgcccgcgc
     1801 ccggctcgaa gtacataccg cccacggttt tcagggcgat gcccgggatc tgatgctgtt
     1861 cagcctctgt gccggcgccg acctgccggc cggagcccgc gccttcctgc gcgagaccgg
     1921 ccatctcgtc aacgtcgccg tcagccgggc ccgcgccgtc tgccacgtgt tcggcgatct
     1981 cgactacgcc gcccgctgcg gcatcccgca cctcgaagcc ctgctcgcgc gccgacgcac
     2041 cgcacccgcc gggccggccg gcttcgagtc accgtgggag gaacggctgc accgcgccct
     2101 cgctgcccgc ggcgtgcaga cgattgcgca atacccggtt gccggccgcc ggctcgatct
     2161 ggcactgctg agcgatgccg tgaagctcga catcgaagtg gacggcgacc gctaccaccg
     2221 cgatcccgac ggccggcgca aggccgccga tctgtggcgc gaccatcaac tgcgcgcgct
     2281 cggctggcgg gtcgtgcgtt tctgggtcta cgaactgcgg gagaacatgg atgagtgcgt
     2341 cgaacgcgtc atcgccggca cccgaccggc cgggccatga gcccatcccc ccgtctgccg
     2401 cgccgccggc ctggccgcgc gtgcagctgg cactcggact ggccagcgcg ctcgcgctcg
     2461 gcagcttctg cagcgtgctg ttcggcgtgg tcggcgacgc gagcgagcgc cgcaactggc
     2521 aggcgcagcg cgtcgggcac ctcgatgcgc tcggcacgct cggtaccgaa cgcagccgcc
     2581 tcgaacggga actcgccgag ctgggccgca accgcgaggc actcgccggc gatctggcgc
     2641 gcctgcgcac cgagaccgaa tccgggcagg ccgccgcgac ccgcctgcgc gaggaagccg
     2701 ggaagctgcg tcaggaggtc accgacaacc gcgccgaact ggaagccgcc ggccgccggc
     2761 gcgatggcct cgccagcgcc atcaggaatg ccgaagccac gctgaccggt ctcaacgacc
     2821 agcgggaaac cgccgaacgc cagagcggcg aagccgagga gcgccgccgt gccgccgccg
     2881 aggccgaacg cagcgcccgg gcgcagcagg gcaaggccga gcagggccgc gacgcggcgc
     2941 tggcccagca gaaggaagcc gagcggcggc tcgggcaagc ggagcaggaa ctgaagacgg
     3001 ccgcggaacg ggtcgacggc ctgcatggcc aggaaaccga gctgaagcgg accgtcacca
     3061 ccgccggtgc cgagcgcgat cgcctgaagg acgaggcaaa gcgactggaa caggaactcg
     3121 cgcggctcga ccagcagcgc cagcaactcg agcgcgacac ccgcggtgcc gccgatgcgc
     3181 agcgcgctgc cgaagccgcc cgccagcagc aggagcgcgt gaaccgcgat ctgcagacgg
     3241 cccaggccgc gctgtcgaat gcgcagggcc agctcggcga aacccggggc cagcagaccc
     3301 agctcgccga ggaagtcgca cgcctgcgcc ggcagaagac cgaactcgat ggcacggtca
     3361 gtgccgccgc cgaagcgcag cgcgccgctg acggtgcccg tcagcagctc gaacggctga
     3421 atcgcgatct gcagacggcc caggccgcgc tgtcgaatgc gcagggccag ctcggcg
//
LOCUS       DAOQIE010000373         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17512_length_3449_cov_7.140837, whole genome shotgun sequence.
ACCESSION   DAOQIE010000373 DAOQIE010000000
VERSION     DAOQIE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17512_length_3449_cov_7.140837"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1091
                     /locus_tag="PK144_16955"
     CDS             168..1091
                     /locus_tag="PK144_16955"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNF67462.1"
                     /translation="MATMDDRDGVIWLDGELVPWREAKTHVLTHTLHYGMGVFEGVRA
                     YQTERGAAIFRLQAHTDRLFRSAHIVGMPMTYTKDEINAACCAAVRENQLESAYIRPM
                     CFYGSEGMGLRADNLKVHTMVAAWPWGAYLGAEGMEKGIRIRTSSFTRHHVNITMCKA
                     KANGNYMNSMMALQEALRDGYDEALLLDVEGYVAEGSGENLFLVRDGVLYTPELTSCL
                     DGITRDTIIRFCADLGLTLKEKRITRDEVWIADEAFFTGTAAEVTPIREVDGRAIGIG
                     RRGPVTERLQKLYFDQVHGRREQYPEWQTLV"
     gene            1179..1373
                     /locus_tag="PK144_16960"
     CDS             1179..1373
                     /locus_tag="PK144_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNF67463.1"
                     /translation="MSAQPAAKPANTENRYEVSRKDLPLSCPMPDMALWNSHPKVYLP
                     IEDKGYAKCPYCGADYVLKD"
     gene            1381..1944
                     /locus_tag="PK144_16965"
     CDS             1381..1944
                     /locus_tag="PK144_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNF67464.1"
                     /translation="MDFAATLAAHAGVISGLLPLAAQLEALAARMRTTLDAGGRIFWL
                     GNGGSAADSQHLAAELVVRYRYDRRALASIALTTDTSILTAHANDFGFETVFSRQLEA
                     LARPGDLVMAISTSGTSPNVLAAARSARALGCWCVGWTGQSGGTLAELADECLRVPST
                     VTARIQEAHILIGHWLCERIEAGLTGG"
     gene            2077..>3449
                     /gene="hldE"
                     /locus_tag="PK144_16970"
     CDS             2077..>3449
                     /gene="hldE"
                     /locus_tag="PK144_16970"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HNF67465.1"
                     /translation="MPMRLPDFSRARVLVAGDLMLDRYWSGPARRISPEAPVPVVHVS
                     ELDDRPGGAGNVALNVAALGGRAWVCAPVGADEAAAVLRARLEAAGVGCAFEVVPQAA
                     TITKLRVLARHQQLIRLDFEDGFPDFEPARLAAGVTALLDAAGIVVCSDYAKGALRDV
                     AAVIAAARTAGRPVLVDPKGTDYARYAGALLITPNEAEFAAVAGEWADEAGLVAKARR
                     LAADCGIGHVLITRSERGMTLVAADGAPAQHIPTRAREVFDVTGAGDTVIATLAAALA
                     AGSPLADAVQLANHAAGIAVGKLGTATVTPLELAAALTPARPPAAGVVERTALLEELA
                     AARARGERIVMTNGCFDLLHAGHVRYLAEAAALGDRLIVAVNDDASVRRLKGSERPIN
                     PLAARMEVLAALRAVDWVVPFTEDTPRALIAQLLPDVLVKGGDYRPEDIAGGAEVIAA
                     GGEVIVLG"
BASE COUNT          489 a         1318 c         1168 g          474 t
ORIGIN      
        1 tcgagtgccg gccggcgagc ggcaccgtcc agcacgtgcg cctgcgcgcc tgagccctgc
       61 tgcccggtgt cgcacgttct gtggtgaaat accgcccctc ccgaccacca gcagacatgc
      121 gcagcgcggc gccgatgcag cgccgcgcgc accgagggct tgcgaacatg gcaacgatgg
      181 acgatcgcga cggcgtgatc tggctcgacg gcgaactggt gccgtggcgc gaggccaaga
      241 cgcacgtgct cacccacacg ctgcactacg gcatgggcgt gttcgagggc gtgcgcgcct
      301 accagactga acgcggcgcg gcgatcttcc ggctgcaggc gcacaccgac cggctgttcc
      361 gctcggcgca catcgtcggc atgccgatga cctacacgaa ggacgagatc aacgccgcct
      421 gctgcgccgc ggtgcgcgag aaccagctcg aatccgccta catccggccg atgtgcttct
      481 acggctccga gggcatgggc ctgcgcgccg acaacctcaa ggtgcacacc atggtcgccg
      541 cctggccgtg gggcgcctac ctcggcgccg agggcatgga gaagggcatc cgcatccgca
      601 cctcctcgtt cacccgccat cacgtcaaca tcacgatgtg caaggccaag gcgaacggca
      661 actacatgaa ttcgatgatg gcgctgcagg aggcgctgcg cgacggctac gacgaggcgc
      721 tgctgctcga tgtcgagggc tacgtcgccg agggctcggg cgagaacctg ttcctggtgc
      781 gcgacggcgt gctgtacacg cccgaactca cctcctgcct cgacggcatc acccgcgaca
      841 ccatcatccg cttctgcgcc gatctcggtc tgacgctgaa ggaaaagcgc atcacccgcg
      901 acgaggtctg gatcgccgac gaggccttct tcaccggcac cgccgccgag gtcacgccga
      961 tccgcgaggt cgacggccgt gcgatcggca tcggccgtcg cggcccggtc accgagcgcc
     1021 tgcagaagct gtacttcgat caggtccacg gccgccgcga gcagtatccg gagtggcaga
     1081 cgctggtctg aaccctgtcc tgttcctgga ccgcggcgcc cgcgcgtcgc ggcccgcatg
     1141 ccgatgacaa cctgcacacc cgaggggaga gtcacaccgt gtccgcgcag cccgccgcca
     1201 agcccgccaa caccgaaaac cgctacgaag tcagtcgcaa ggacctgccg ctgtcctgcc
     1261 cgatgccgga catggcgctg tggaactcgc acccgaaggt ctatctgccg atcgaggaca
     1321 agggctacgc caagtgcccg tactgcggcg ccgactacgt gctgaaggac tgacgcgccc
     1381 gtggacttcg ccgcgacgct ggccgcgcac gccggagtca tctccggcct gctgccgctc
     1441 gccgcgcagc tcgaagcgct cgccgcccgc atgcgcacga cgctcgatgc cggcgggcgc
     1501 atcttctggc tcggcaacgg cggcagcgcc gccgacagcc agcatctggc cgccgagctg
     1561 gtggtccgct accgctacga tcgccgcgcg ctcgcctcga tcgcgctgac caccgacacc
     1621 tcgatcctga ccgcgcacgc caacgacttc ggcttcgaga ccgtgttctc gcgccagctc
     1681 gaagccctgg cccggcccgg cgatctggtc atggcgatct ccacgtccgg caccagcccg
     1741 aacgtgctcg ccgccgcccg cagcgcccgc gcgctcggct gctggtgcgt cggctggacc
     1801 ggccagagcg gcggcacgct cgcggaactc gccgacgaat gcctgcgcgt gccctcgacc
     1861 gtcaccgccc gcatccagga agcccacatc ctgatcggcc actggctgtg cgagcggatc
     1921 gaggccgggc tgacgggcgg ctgagtccgc ccgccgcgcc tcgcgggttt cccgggcctg
     1981 ctgccgcggg cggggctgcc aggatgccgg gcccggtggt gatggacccg gcggctgccg
     2041 cccgtgcccc tgtttccacc tgatccccgg ggaccgctgc cgatgcgcct gcccgatttc
     2101 tcccgcgccc gcgtgctcgt cgccggcgac ctgatgctcg atcgctactg gtccggcccg
     2161 gcgcggcgca tctcgcccga ggcgccggtg ccggtcgtgc atgtctcgga attggacgac
     2221 cgcccgggcg gagccggcaa cgtcgcgctg aacgtcgccg cgctcggcgg ccgcgcctgg
     2281 gtgtgcgcgc cggtcggggc cgacgaggcc gccgcagtgc tgcgtgcgcg gctcgaagcc
     2341 gccggcgtcg gctgcgcgtt cgaggtcgtg ccgcaggccg cgaccatcac caagctgcgc
     2401 gtgctcgccc gccaccagca gctgatccgg ctcgacttcg aggacggctt tccggacttc
     2461 gagccggcgc gcctcgccgc gggcgtcacg gcgctgctcg atgccgccgg catcgtcgtc
     2521 tgctcggact acgccaaggg cgcgctgcgc gacgttgccg ccgtgattgc cgccgcccgc
     2581 accgccggcc ggccggtgct ggtcgatccg aagggcacgg attacgcgcg ctacgccggc
     2641 gcgctgctga tcacaccgaa cgaggccgaa ttcgcagcgg tcgccggcga gtgggccgac
     2701 gaggccggac tggtcgcgaa ggcccggcgt ctggcggccg actgcggcat cggccatgtg
     2761 ctgatcacgc gcagcgagcg cggcatgacg ctggtcgccg ccgacggcgc accggcgcag
     2821 cacatcccga cccgcgcccg cgaggtgttc gacgtgaccg gtgccggcga caccgtgatc
     2881 gccacgctcg ccgccgcgct cgccgccggc agcccgctcg ccgacgccgt gcagctcgcc
     2941 aaccacgccg ccggcatcgc cgtcggcaag ctcggcaccg cgacggtcac gccgctggaa
     3001 ctggctgccg cactgacgcc ggcgcgtccg ccggccgcag gcgtggtcga gcgcacggcg
     3061 ctgctcgaag aactcgccgc cgcccgcgcc cgcggcgagc gcatcgtcat gaccaacggc
     3121 tgcttcgacc tgctgcacgc cggccacgtc cgctatctcg ccgaggctgc cgcgctcggc
     3181 gaccggctga tcgtcgccgt caacgacgac gcctcggtgc gccgcctgaa aggcagcgaa
     3241 cgaccgatca acccgctcgc cgcgcgcatg gaagtgctcg ccgcgctgcg cgccgtcgac
     3301 tgggtcgtgc ccttcaccga ggacacgccg cgcgcgctga tcgcgcaact gctgcccgac
     3361 gtgctggtca agggcggcga ctaccggccc gaggacatcg ccggcggcgc cgaggtcatc
     3421 gccgccggtg gcgaggtcat cgtgctcgg
//
LOCUS       DAOQIE010000374         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17564_length_3442_cov_7.053440, whole genome shotgun sequence.
ACCESSION   DAOQIE010000374 DAOQIE010000000
VERSION     DAOQIE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17564_length_3442_cov_7.053440"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PK144_16975"
     CDS             <1..1083
                     /locus_tag="PK144_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNF67466.1"
                     /translation="PAEINLRAGDIANMGFAEAVDCPVWLVADIDRGGVFASLVGTLE
                     LLSASERARVQGFVINRFRGDISLLQPGLDWLEARTGVPVLAVLPYLHGLALDAEDAI
                     ARDADVQDGRPQLRVIAPVLPRISNHTDLDPLRRHPQVELRWIGPGDPIPPADLIVLP
                     GSKSVQADLTALRARGWDTAIRRHLRYGGRVLGLCGGLQMLGERLHDPLGLEGAPGSA
                     PGLGLLALETTLAPEKTLRRVRGRFIGIDAGATGAPVEGYEIHAGVSSGPALARPLLR
                     LDDGRLDGAVSADGLIRASYVHGLFDQPEALLALLDWAGLPQAAALDPGAQREAALER
                     LADALEAALRPGTLAQWFGLAEPAAT"
     gene            1129..1443
                     /locus_tag="PK144_16980"
     CDS             1129..1443
                     /locus_tag="PK144_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67467.1"
                     /translation="MTVAPRRRIAVALVLATGLLTGASRADSFRLDNGRLITDGMSRI
                     EVLSRAGPPLTTTLLSVGINGMPSREQWLYRAPGSISGAYWISVIFEGDTAIEVRAER
                     ER"
     gene            complement(1448..3325)
                     /locus_tag="PK144_16985"
     CDS             complement(1448..3325)
                     /locus_tag="PK144_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF67468.1"
                     /translation="MKALSSLPPSRRLPLLMALLVAVALLVSSVLGALVAHWESVDSQ
                     LAEVRNLADNGRKTFARTLKDVHADLRTLAGSTTVRAALIGLSRAFEDQGPQARERLQ
                     QLYLQDNPNPKGQKQRLNDAGDGSDYSRLHAYLQPWLTNFTEDRGYYDLFLIDPAGKI
                     LYTVFKESDFASRLDSPALADSGLAAVYKRAQQLTDTGHYVFADFAPYAPSNGDPAAF
                     IGVPVRSGGSGGRLLGVLVLQLPIEKFNDTLATLGSEGKRVFAEYGRSAAGVIVDDFV
                     HETDAEGRGVRRIRLPVEIDGVRWYVVGEVHDATLYRPGLILGATLLAMALLAVAVAV
                     PLARRLVRSLWTPVEALASRFDAEVGRSVEEIGGIASQVEIISGRLAHEATDSGNHIR
                     RLLERIDTVSASVGTAAAAVEQISVSIGNINSATGTSELRMSHARERVQAAEAAIGEL
                     LGATAEIDKVLRVIEGVAAQTRLLSLNAVIESARAGEYGKGFAVVANEVKALSHQTSG
                     ATKEVGGEVEAIRAGSAHSAEGLQGVASAVDQVGEAVRDITGMIGEQRIAVTEIAGMV
                     GAVSGEAQAVAREARDVGASAERTGEEARSMRELAGRLLGDVERTRSRTQAFLAGLRA
                     L"
BASE COUNT          481 a         1259 c         1200 g          502 t
ORIGIN      
        1 ccggccgaga tcaacctgcg cgccggcgac atcgccaaca tgggcttcgc cgaggccgtc
       61 gactgcccgg tgtggctggt cgccgacatc gaccgcggcg gcgtgttcgc gagcctcgtc
      121 ggcacgctgg aactgctgtc ggcctccgaa cgcgcgcgcg tgcagggctt cgtgatcaac
      181 cgcttccgcg gtgacatttc actgctgcag ccgggcctcg actggctgga ggcgcgcacc
      241 ggcgtgccgg tgctggcggt gctgccgtat ctgcacggcc tcgcgctcga tgccgaggac
      301 gccattgccc gggatgccga cgtgcaggac ggcaggccgc aactgcgcgt gatcgcgccg
      361 gtgctgcccc gcatcagcaa ccacaccgac ctcgatccgc tgcgccggca tccgcaggtg
      421 gagctgcgct ggatcggccc cggcgacccc atcccgccgg ccgacctgat cgtgctgccc
      481 ggctcgaaaa gcgtgcaggc cgatctcacg gcgctgcgcg cccggggctg ggacacggcg
      541 atcaggcgcc acctgcgcta cggcgggcgc gtgctcggcc tctgcggcgg gctgcagatg
      601 ctcggcgaac gcctgcacga cccgctcggc ctcgaaggcg cgccgggcag cgcgccgggg
      661 ctcggactgc tcgcgctcga aaccacgctt gcgccggaaa agaccctgcg ccgcgtgcgc
      721 ggccgtttca tcggcatcga cgctggcgcg accggcgcac cggtcgaggg ctacgagatt
      781 cacgccggcg tctcgagcgg cccggcgctg gcaaggccgc tgctgcgcct cgatgacggc
      841 cgcctcgatg gcgcggtgtc cgcagacggc ctgatccgcg ccagctacgt gcacggcctg
      901 ttcgatcagc ccgaagccct cctcgcgctg ctcgactggg ccgggctgcc gcaggccgca
      961 gcgctcgacc cgggtgcgca gcgcgaggcg gcgctcgaac ggctcgccga tgcgctcgaa
     1021 gcggcgctgc ggccgggcac gctggcgcag tggttcggac tcgcggagcc ggccgcgacc
     1081 tgaagccgcg cacctatgct tgcaggacgc cctccggagc ccgccgcaat gaccgtcgcc
     1141 ccgcgccgcc gcatcgccgt cgccctcgtg ctcgccaccg ggctgctgac gggcgcgagc
     1201 cgcgccgatt ccttccgcct cgacaacggc cggctgatca ccgacggcat gagccgcatc
     1261 gaggtgctgt cccgggccgg gccgccgctg acgacgacgc tgctgtcggt cggcatcaac
     1321 ggcatgccct cgcgcgaaca gtggctgtac cgcgcgccgg gttcgatttc cggcgcttac
     1381 tggatcagcg tgatcttcga gggcgacacc gccatcgagg tgcgcgccga gcgcgagcgc
     1441 tgaagcctca aagcgcccgc aagccggcca ggaaggcctg cgtgcgcgag cgcgtgcgct
     1501 cgacatcgcc gagcagacgg ccggcaagct cccgcatcga gcgcgcctcc tcgccggtgc
     1561 gctcggccga ggcgccgaca tcgcgcgcct cgcgggcgac ggcctgcgcc tcgccggaca
     1621 cggcgccgac catgccggcg atctcggtca cggcgatgcg ctgctcgccg atcatgccgg
     1681 tgatgtcgcg caccgcctcg ccgacctgat cgacggcgct ggcgaccccc tgcagaccct
     1741 cggcgctgtg tgcggagccg gcgcggatcg cctcaacctc gccgccgact tccttggtcg
     1801 cgcccgaggt ctggtgcgac agcgccttga cctcgttggc gaccaccgcg aagcccttgc
     1861 cgtactcgcc ggcacgcgcc gattcgatga cggcgttcag cgacagcagc cgcgtctgtg
     1921 cggcgacgcc ctcgatgacg cgcagcacct tgtcgatctc ggccgtggcc ccgagcagtt
     1981 cgccgatcgc ggcttcggcg gcctgcacgc gctcgcgggc atggctcatg cgcagttcgc
     2041 tggtgccggt ggcgctgttg atgttgccga tcgagacgct gatctgctcg accgccgccg
     2101 cggcggtgcc gacgctggcc gagacggtgt cgatgcgttc gagcaggcgg cggatgtggt
     2161 tgccggaatc ggtggcctcg tgcgccagcc ggccggagat gatctcgacc tggctggcaa
     2221 tgccgccgat ttcctcgacg ctgcgcccga cctcggcatc gaagcgcgag gccagcgcct
     2281 cgaccggcgt ccacaggctg cgcaccagcc ggcgcgcgag cggcaccgcc accgccaccg
     2341 ccagcagcgc catggcgagc agcgtggcac cgaggatcag acccggccgg tacagcgtcg
     2401 cgtcatgcac ctcgccgacc acgtaccagc gcacaccatc gatctcgacc ggcaggcgga
     2461 tgcggcgcac gccgcggcct tcggcgtcgg tctcgtgcac gaagtcatcg acgatcacgc
     2521 cagcggcgct gcggccgtac tcggcgaaca cgcgcttgcc ctcgctgccg agcgtggcca
     2581 gcgtgtcgtt gaacttctcg atcggcagct gcagcaccag cacgccgagc agccggccgc
     2641 cgctgccgcc gctgcgcacc ggcacgccga tgaaggctgc cggatcgccg ttgctcggcg
     2701 catacggtgc gaagtcagcg aacacgtagt ggccggtgtc ggtcagctgc tgcgcgcgct
     2761 tgtacaccgc ggccagcccg ctgtcggcca gcgccgggct gtcgaggcgc gacgcgaagt
     2821 cggattcctt gaacaccgtg tagaggatct ttcccgcggg atcgatcagg aacaggtcgt
     2881 agtagccgcg atcttcggtg aaattcgtca gccacggctg caggtaggca tgcagccggc
     2941 tgtagtcgga gccatcgccg gcatcgttga ggcgctgctt ctggcccttc ggattcggat
     3001 tgtcctggag gtagagctgc tgcaggcgct cacgcgcctg cggcccctgg tcctcgaagg
     3061 ccctggacag gccgatcagc gccgcacgca ccgtcgtcga gccggccagc gtgcgcaggt
     3121 cggcatgcac gtccttcagg gtccgcgcaa aggtcttgcg gccgttgtcg gcgaggttgc
     3181 gtacctcggc cagttgcgaa tcgaccgatt cccagtgcgc caccagagcg ccgagcacgg
     3241 aagacaccag cagcgccacg gccaccagca acgccatcag cagcggcaga cggcggctcg
     3301 gcggcagcga ggacagggct ttcatcgacg gcgactccat gcggttgcgc attccggcgc
     3361 agcgaaggga ggcgcagtct gacagtcgat tccggcctga ccagagggtt ttacaaggct
     3421 tgatggttgg cgccgcaagc gc
//
LOCUS       DAOQIE010000375         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17759_length_3415_cov_7.122024, whole genome shotgun sequence.
ACCESSION   DAOQIE010000375 DAOQIE010000000
VERSION     DAOQIE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_3415_cov_7.122024"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..2424
                     /locus_tag="PK144_16990"
                     /pseudo
     CDS             208..2424
                     /locus_tag="PK144_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
     gene            2574..3020
                     /locus_tag="PK144_16995"
     CDS             2574..3020
                     /locus_tag="PK144_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNF67469.1"
                     /translation="MAIGCDMSGHDADFYGWTREQAAALRAGRFDTLDREALAEEIES
                     MGNRERRELVNRLAVLLCHLLKWQYQPERRGSSWRSTIREQRRQVALILRDNPSLRPK
                     LPELLADAYEYGAEDALEETGFFKAPWSQQCPFGMEQVLAADFWPV"
     gene            3156..>3415
                     /locus_tag="PK144_17000"
     CDS             3156..>3415
                     /locus_tag="PK144_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67470.1"
                     /translation="MGMISGFLAVLVFLISLAMSLPADAAGLEVREPSRMVVASSGAD
                     VPDAFCKGLALAPKQVRVFFGVPMWQGRATLPDSFVIQSIVKT"
BASE COUNT          589 a         1103 c         1159 g          564 t
ORIGIN      
        1 gcctgcacca cggcgttggt ggccaccgtg ccccttcctg cagagtctcc cgaggaactc
       61 gtcgaggcga ggcctgggca attcgcgcca ccgccccctc gcaagacgcc agccttccgt
      121 cggccggagt accacgcaga ccgggccacg ttcgacttcc tgcgccaggc cgctcggagc
      181 ggaggccagg tcacctggga gcagctcgac gccatccggc gcatggaccg cgcgctgcgc
      241 gagttccgcg tgcgcgggct gtcgacgaac ctcgccttcc tcgtcaacgt catcaaccac
      301 gcgcagttcc gggacggcag ctacatcacg cgcttcatcg acgagacgcc ggagctcttc
      361 cacttcccga agcggcgcga ccgcgccacg cgcctgctgc gcttcatcgc cgagaccacc
      421 gtcaacggca accccgaggt cgacggccgt gccccggccg agagccgcca gccggcgctg
      481 ctgccgagcg cgcaccccgg tgccccgaag cccggcacgc gcgacaggct gctggaaatc
      541 ggccccgagg cgttctcgaa gtggatgcgc gccgaaacgc gcgtgctgct gaccgacacg
      601 acgatgcgcg atgcgcacca gtcgctgctc gcgacgcgca tgcgcaccga cgacatgctg
      661 acgatcgcgc cgtactacgc cgcgctgctg ccggagctgt tctcgctcga atgctggggc
      721 ggcgcgacct tcgacgtggc gatgcgcttc ctgaaggaag acccgtggga gcgcctcgcc
      781 ggcctgcgcg agcgcgtgcc gaacctcctg ttccagatgc tgctgcgcgg cgccaacgcc
      841 gtcggctaca ccagctatcc ggacaacgtc gtgcgcttct tcgtgaagca ggccgccgag
      901 tgcggcatgg acgtgttccg cgtgttcgac agcctgaact gggtcgagaa catgcgtgtg
      961 gcgatggatg ccgtgctcga atccggcaag ctctgcgagg cgacgctctg ctacaccggc
     1021 gacatgctcg cggccggtcc gaacaagtac gacctcaagt actacgtgac gatggcgaag
     1081 gagctggaga aggccggcgc acacatcctc gcggtcaagg acatggccgg cgtcgcccgc
     1141 ccggcggcga tccgcaagct cgtcacgacg ctgcgcgagg aaaccggcct gccgatccac
     1201 ttccacacgc acgacacctc cggcatcagc gccgccagcg tgctcgcggc ggtcgaggcc
     1261 ggctgcgatg ccgtcgatgg cgcgatggac gcgatgagcg gcctgacctc gcagccgaat
     1321 ctcggctcga tcgccgtcgc gctcgccgac accgagcgcg atccgggcgt cgaccgctcc
     1381 gcgatgcgcg cgatctcgca ctactgggaa ggcgtgcgcc gcttctacac cggcttcgag
     1441 agcgacatcc gctccggcac ggccgatgtc taccgccacg ccatgccggg cggccagtac
     1501 accaacctgc gcgagcaggc gcgttcgctc ggcatcgatc accgctggga cgaggttgcc
     1561 gaggcctacg cgcaggtcaa cgagctgttc ggtgacatcg tcaaggtgac gccgacctcg
     1621 aaggtcgtcg gcgacatggc gctgatgatg gtcacgcagg gtctgagtgc tgccgacgtg
     1681 caggacccgg cccgcgagat cgccttcccc gagtcggtcg tgcagctctt ccacggcgac
     1741 ctcggtcagc cgccgggcgg ctggccggaa acgctgatgc gcaaggtgct caacggcaag
     1801 gaggcgctga ccgtgcgccc cggcgccgac ctgccgccgg ccgacctcga agcgatccgc
     1861 aaggaagccg ccacgaagac cggccgcgag atcggcgact acgagctggc ctcgtacctg
     1921 atgtacccga aggtctacac cgactacgcg aaggcgcgca gccagttcgg cgacaccgcg
     1981 ctgctgcgca cgccggtgtt cttctacggc ctggagatcg gcgcggaaac gacgatcgag
     2041 ctcgaaaagg gcaagacgct gctggtgcgc ctgctcgcgg tcagcgagcc ggacgacgaa
     2101 ggcttccgca aggtgttcct ggagctgaac ggccagggcc gcagcgtcaa ggtgcagcag
     2161 aagggcctgg aggcgatgtc ccgcgcccac ccgaaggccg aggacggcaa tccgcatcac
     2221 gtcgccgcgc cgatgccggg catggtcatc agcgtcgcgg tgcgcccggg cctgaaggtc
     2281 gagcgcggcg atgcgctggt gtcgatcgag gcgatgaaga tggaatcggt gatccgcgcc
     2341 gaacgccccg gcgtcgtcaa ggaagtccac gtcgccgtcg ggacggtggt ggatgcgaag
     2401 gatctgatgg tggtgatggg ctgagccgga tggctgaacg ggcagggagg cccggtgcag
     2461 aagaaacaaa gccgatccgg gtccggaccc ggggcggctt ttgttctggc gatccgaaag
     2521 tctatgctga tcacattcct ccccgccgac gggcgccagt cacgattcgg caaatggcca
     2581 tcgggtgtga catgagcgga catgacgcag atttctacgg ctggacccgg gaacaggccg
     2641 ccgccttgcg ggccggccgt ttcgatacgc tggatcgtga ggcgctggcc gaggaaatcg
     2701 aaagcatggg caaccgtgaa cggcgcgaac tggtcaatcg actggcggtg ctgctttgtc
     2761 atttgctgaa atggcaatat cagccggaac gtcggggttc gagctggcga tcgacgatcc
     2821 gtgagcagcg gcggcaggtg gcgctgattc tgcgcgacaa tccgagtctg cggccgaaac
     2881 tgccggaatt gctggcggat gcgtatgaat atggtgccga ggatgcgctg gaggaaaccg
     2941 gcttcttcaa ggcgccctgg tcgcagcagt gtccgttcgg gatggagcag gtgctggcgg
     3001 cggacttctg gccggtgtga agggtgacgg gtgctccctg aactgatttg ccatgggtcc
     3061 ggctgatcag ggagttgaag tattcctgtc actcgccacc gtgttccggt atccgtggtt
     3121 tcataccgat aaccatccgg ggcgagtacc gcagtatggg gatgatttcc ggttttctgg
     3181 cggtgctggt atttttaatc agcctggcca tgtccttgcc ggcggatgcc gcagggcttg
     3241 aagtaagaga accatccaga atggttgttg cttcaagcgg cgccgatgtt ccggatgcat
     3301 tctgcaaagg gttggcgctt gctccgaagc aggtgcgtgt ttttttcggc gtgcccatgt
     3361 ggcagggtcg tgcaacgcta cctgattcct ttgtaattca gagcattgtc aagac
//
LOCUS       DAOQIE010000376         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17908_length_3397_cov_6.361460, whole genome shotgun sequence.
ACCESSION   DAOQIE010000376 DAOQIE010000000
VERSION     DAOQIE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17908_length_3397_cov_6.361460"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK144_17005"
     CDS             complement(<1..217)
                     /locus_tag="PK144_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67471.1"
                     /translation="MWFVLLLALWTAVSVYLNERLQAHARATAQQMQVIAAARARALD
                     EWWLLHDSNAGLLALAAGAHAPVHRLRL"
     gene            533..3397
                     /gene="gcvP"
                     /locus_tag="PK144_17010"
     CDS             533..3397
                     /gene="gcvP"
                     /locus_tag="PK144_17010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNF67472.1"
                     /translation="MTDPRRPLADLEPDTDFVRRHIGPDAAGQAAMLATLGVGSLAAL
                     IDEVVPADIRRSDAMAIAPAVDEAAVLAELRSVAARNTVLKSLIGQGYYGTHVPPVVL
                     RNVLENPAWYTAYTPYQPEISQGRLEALLNFQTLICELSGLDIANASMLDEATAAAEA
                     MTLLQRGGRSKSATFFVADDVFAQTLAVLRTRAEPLGIRIVTGDPLSVLPGLDCFGAL
                     LQYPGANGAVRDYRALAETLHARGGLLAVAADPLALCLLAPPGAWGADVVVGSSQRFG
                     VPMGFGGPHAGFLATRDALKRVMPGRLVGVSIDSHGAAALRLALQTREQHIRREKATS
                     NICTAQVLLAVMASLYACWHGPEGLKRIAQRVHRLTAIFAAGLRGQGFTVAPEAFFDT
                     LTVATGAQTAAIHARARAAGYNLREIDEATLGLSFDETVTRTDLETLWGVFAEGEAAL
                     PELDALDAAGAGGLPAALLRSGPFLTHPVFSAHRSETDMLRYLRRLADRDIALDRAMI
                     PLGSCTMKLNATTEMIPVTWPEFAQLHPFAPAAQTRGYAQLIDELEAMLCAATGYAAV
                     SLAPNAGSQGEYAGLLIIRKYHESRGEGHRDVCLIPSSAHGTNPASAQMAGMRVVVVA
                     CDRNGNVDLGDLRTKAAQHAAHLAAIMITYPSTHGVFESGVRELCEIVHAHGGQVYID
                     GANLNALVGLAAPGAFGGDVSHLNLHKTFCIPHGGGGPGVGPVAVAAHLAEFLPGRGE
                     QAIGAVTAAPGGSASILPISWTYIRLMGAEGLSSATGHAILAANYIATRLAPHYPVLY
                     TGPNGRVAHECIIDLRPIKDSTGISVEDVAKRLIDYGFHAPTMSFPVPGTLMIEPTES
                     EPKSELDRFIDAMISIREEIRAVETGALDADDNPLKHAPHTAQSVCADHWPHAYPREQ
                     AAYPVPGLRTAKYWPPVGRVDNVHGDRNLFCSCVPVDDYA"
BASE COUNT          504 a         1271 c         1146 g          476 t
ORIGIN      
        1 cgagccgcag gcggtgtacc ggcgcgtgcg cgccggcggc cagcgcgagc agcccggcgt
       61 tgctgtcgtg cagcaaccac cattcatcga gcgcccgcgc gcgcgccgcg gcgatcacct
      121 gcatctgctg cgcggtggca cgcgcgtggg cctgcagccg ctcgttcagg tacacggaga
      181 ccgccgtcca cagcgcgagc agcagcacga accacaccag gatcaggggc agcgggggtg
      241 gacgtcggct ggcaggaaga gagggcatcg gcgagggggg gcttgttgca cgcgggtgag
      301 ccgtacgacg aatcacggcg tacgggcagc taccttaccg ccggttaaga ttcggtaaac
      361 ccacacttta tgaatgtttg cgcgtacccc gtccgggcgg acggcgtaac gccggctgaa
      421 cagccgattt gcgcggcccg caccggcacc gggacaatgc cgcgcctgca gtgacggccc
      481 ctgccgtgtg cagccgtcgc ctccgcttcc cagtcccccg aggtttccga ccatgaccga
      541 cccgcggcgc ccgctcgccg atctcgaacc agacaccgat ttcgtccgcc gtcacatcgg
      601 ccccgatgcc gccggacagg cggcgatgct ggccacgctc ggcgtcggct cgctggcggc
      661 gctgatcgat gaagtcgtgc cggccgacat ccgccgctct gatgcaatgg cgatcgcccc
      721 ggccgtcgac gaggccgcgg tgctcgccga gctgcgcagc gtcgccgcgc gcaacacggt
      781 gctgaagtcg ctgatcggcc agggctacta cggcacgcac gtgccgccgg tggtgctgcg
      841 caacgtgctg gagaacccgg cctggtacac ggcctatacg ccgtaccagc cggaaatctc
      901 ccagggccgg ctcgaagcgc tgctgaactt ccagacgctg atctgcgagc tgagcggtct
      961 cgacatcgcc aatgcctcga tgctcgacga ggccaccgcc gccgccgagg cgatgacgct
     1021 gctgcagcgc ggcgggcgct cgaagagcgc gaccttcttc gtcgccgatg atgtgttcgc
     1081 gcagacgctc gccgtgctgc gcacgcgcgc cgagccgctc ggcatccgca tcgtcaccgg
     1141 cgatccgctc agcgtactgc ccggcctcga ctgcttcggc gcgctgctgc aataccccgg
     1201 tgcaaacggc gcggtgcgcg actaccgcgc gctcgccgag accctgcacg cgcgcggcgg
     1261 cctgctcgcg gtcgcggccg atccgctggc gctgtgcctg ctcgcgccgc ccggagcctg
     1321 gggcgccgat gtcgtggtcg gcagcagcca gcgtttcggc gtgccgatgg gcttcggcgg
     1381 tccgcacgcg ggcttcctcg ccacgcgcga tgcactgaag cgcgtgatgc cggggcgtct
     1441 ggtcggcgtg tcgatcgaca gccacggcgc cgcggcgctc aggctggcgc tgcagacccg
     1501 cgagcagcac atccgccgcg agaaggcgac ctcgaacatc tgcaccgcgc aggtgctgct
     1561 cgcggtgatg gcgagcctgt acgcctgctg gcacggcccc gagggcctga agcgcatcgc
     1621 gcagcgcgtg caccggctga ccgcgatctt cgccgccggc ctgcgcggcc agggcttcac
     1681 ggttgccccc gaagccttct tcgacacgct gaccgtcgcc accggcgcgc agaccgccgc
     1741 gatccacgcc cgcgcgcgcg ccgccggcta caacctgcgc gagatcgacg aggcgacgct
     1801 cgggctgtcc ttcgacgaga ccgtcacgcg caccgacctc gaaacgctgt ggggcgtgtt
     1861 cgcggaaggc gaggcggcgc tgccggaact cgacgcgctc gatgccgccg gcgccggcgg
     1921 gctgccggcc gcgctgctgc gcagcggccc gttcctgacg catccggtgt tctcggcgca
     1981 ccgcagcgag accgacatgc tgcgctacct gcgccggctc gccgaccgcg acatcgcgct
     2041 cgaccgcgcg atgatcccgc tcggctcctg cacgatgaag ctcaacgcca ccaccgagat
     2101 gatcccggtc acctggccgg aattcgcgca actgcacccg ttcgcgccgg ccgcccagac
     2161 gcgcggctat gcgcagctga tcgacgaact cgaagcgatg ctgtgcgccg cgaccggcta
     2221 cgccgccgtg tcgctcgcgc cgaacgccgg ttcgcagggc gagtacgcgg gtctcctgat
     2281 catccgcaag taccacgaga gccgcggcga ggggcatcgc gatgtctgcc tgatcccgtc
     2341 ctcggcgcac ggcaccaatc cggcctcggc gcagatggcc ggcatgcgcg tcgtcgtcgt
     2401 cgcctgcgac cgcaacggca acgtcgatct cggcgacctg cgcaccaagg ccgcgcagca
     2461 cgccgcgcat ctggccgcga tcatgatcac gtatccgtcg acgcacggcg tgttcgagtc
     2521 cggcgtgcgc gaactctgcg agatcgtcca cgcccacggc gggcaggtgt acatcgacgg
     2581 cgccaacctc aacgcgctgg tggggctggc cgcgcccggt gccttcggcg gcgatgtctc
     2641 gcacctgaac ctgcacaaga ccttctgcat cccgcacggc ggcggcggtc ccggcgtcgg
     2701 gccggtggcg gtcgcggcgc atctggccga gttcctgccc gggcgcggcg agcaggcgat
     2761 cggtgccgtc accgccgcgc cgggcggctc ggcgagcatc ctgccgatct cgtggaccta
     2821 catccgcctg atgggcgccg agggcctcag cagcgccacc ggacacgcga tcctcgccgc
     2881 caactacatc gccacccggc tcgcgccgca ctacccggtg ctctacaccg gcccgaacgg
     2941 ccgcgtcgcg cacgagtgca tcatcgacct gcgcccgatc aaggacagca ccggcatcag
     3001 cgtcgaggac gtggccaagc gcctgatcga ctacggcttc cacgccccga cgatgagctt
     3061 cccggtgccc ggcacgctga tgatcgagcc gaccgagagc gagccgaagt ccgaactcga
     3121 ccgcttcatc gacgcgatga tctcgatccg cgaggaaatc cgcgccgtcg aaaccggcgc
     3181 gctcgacgcc gacgacaacc cgctgaagca cgccccgcac acggcgcaga gcgtctgcgc
     3241 cgatcactgg ccgcatgcgt atccgcgcga gcaggcggcc tatccggtgc cgggcctgcg
     3301 caccgccaag tactggccgc cggtcggtcg cgtcgacaac gtgcatggcg accgcaacct
     3361 gttctgcagt tgcgtgccgg tggatgacta cgcctga
//
LOCUS       DAOQIE010000377         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_17974_length_3387_cov_7.721789, whole genome shotgun sequence.
ACCESSION   DAOQIE010000377 DAOQIE010000000
VERSION     DAOQIE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17974_length_3387_cov_7.721789"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /gene="motA"
                     /locus_tag="PK144_17015"
     CDS             complement(<1..697)
                     /gene="motA"
                     /locus_tag="PK144_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNF67473.1"
                     /translation="MFVIIGYVVIIGCILGGFIMAGGHLGVLMQPVEVLIIVGSAVGA
                     FFVGNSMTVVKEAGGALGACFKGGKYTQALYMETLTLLYLIFAKIRKEGLLALQADID
                     EPHSSKLFEQAPTVLHDHHAVEFITDYLRLLSSGNIELNQLDNLMDLDIETHHHQGER
                     PIKAIQAFADGLPAFGIVAAVLGVVHTMESVGIPPAELGKLIAAALVGTFLGILLAYG
                     FVGPLGSLLTQKLD"
     gene            complement(779..2764)
                     /locus_tag="PK144_17020"
     CDS             complement(779..2764)
                     /locus_tag="PK144_17020"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNF67474.1"
                     /translation="MGPHPHPLLAGQPLAPKGKVVTAAEAVRLIRDGDTLGTGGFVGI
                     GFAEGLACALERRFLETGEPRNLRLVYAAGQGDGAERGLNHLGHEGLVDSVVGGHWGL
                     VPKLQKLAIDGRISAWNLPQGVITHLFRDIAAGKPGTLTRVGLGTFVDPEFGGGKLNE
                     RTTEELVRRLPIDGEDYLFYKAFPINACFLRGTTGDPDGNITMEREALTLEALALATA
                     TRNSGGIVIVQVERIAERGSLSARQVKIPGILVDCVVVAEAREHQQTFAEPYSPAFSG
                     EIRVPVTGIAPLALNERKLIARRAAFELRPNAVVNLGIGMPEGIAGVANEEQVLELLT
                     LTTEPGVIGGLPASGLNFGAAVNTQAIIDQPSQFDFYDGGGLDIAFLGLAQADAEGNL
                     NVSKFGPRLAGAGGFINISQNAKRVVFVGTFTAGGLRLAIGNGQLHIEQEGRERKFVR
                     AVEHRTYSGPVAAARGQKALYVTERCVFELSAGGLVLTEVAPGIDIERDILAHMDFAP
                     LIPQPPRLMDARIFLPATMGLRAGLLELPLEQRFTYDAAQNLFFIDFGGYHVARPEQI
                     EAIRGVVETRLGHLDKRVDTIVNYDDFSIAPELLDAYIEMVRDVVGRFYSGVTRYTTN
                     AFLRMKLGRALSERELTPALYGSRDEARAGLASGSQR"
     gene            complement(2871..3368)
                     /locus_tag="PK144_17025"
     CDS             complement(2871..3368)
                     /locus_tag="PK144_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF67475.1"
                     /translation="MTYRHVLLAAEFDAVGPRMLARVSELLRYGAPRLTLLHVVDERS
                     EGGSELLPTVAGTPEPHRLVVPQGTEENLPLESDTETRLDEAAARFLQSLAQRLGVPG
                     AQLRVVASTSVSTAIIAEARAAGVDLIIVGHHDRHWLDWLVAGTAERVVGAAVCDVLV
                     LHTAD"
BASE COUNT          502 a         1149 c         1199 g          537 t
ORIGIN      
        1 cgtcgagctt ctgcgtcagc aggctgccga gcgggccgac gaagccgtag gccagcagga
       61 tgccgaggaa ggtgccgacc agcgccgcgg cgatcagctt gccgagctcg gccggcggga
      121 tgccgaccga ctccatcgtg tgcacgacac cgagcacggc cgcgacgatg ccgaaggccg
      181 gcaggccgtc ggcgaaggcc tggatggcct tgatcggtcg ctcgccctga tggtggtggg
      241 tctcgatgtc gaggtccatc aggttgtcga gctggttcag ctcgatgttg cccgaggaca
      301 gcaggcgcag gtaatcggtg atgaactcga cggcgtggtg atcgtgcagc acggtcggcg
      361 cctgctcgaa cagcttgctg ctgtgcggct cgtcgatgtc ggcctgcagc gcgagcaggc
      421 cttccttgcg gatcttcgca aagatcagat agagcagcgt cagcgtctcc atgtacagcg
      481 cctgcgtgta cttgccgccc ttgaagcagg caccgagcgc cccgcccgcc tccttgacga
      541 ccgtcatgct gttgccgacg aagaaggcgc cgaccgccga gccgacgatg atcagcactt
      601 ccaccggctg catcagcacg ccgagatggc cgccggccat gatgaaaccg ccgaggatgc
      661 agccgatgat gaccacgtag ccgatgatga cgaacatgcg cgaccttgcg gccccccgcg
      721 ggccgggctt gtcggacgac cgggcgcgtc gcgccgccgc acccggtccg ggccgccgtc
      781 agcgctggct gccggatgcc aggcctgccc gcgcctcgtc tcgactgccg tacagcgccg
      841 gcgtcagctc gcgctcggag agcgcgcggc cgagtttcat gcgcaggaag gcattggtcg
      901 tgtaacgggt gacgcccgaa tagaagcgcc cgaccacgtc cctgaccatt tcgatatagg
      961 cgtcaagcag ttccggggcg atgctgaagt cgtcgtagtt gacgatggtg tcgacgcgtt
     1021 tgtcgaggtg tcccagccgc gtctcgacca ccccgcggat cgcttcgatc tgttcggggc
     1081 gggcgacatg gtagccgccg aaatctatga agaacagatt ctgcgcggca tcgtacgtga
     1141 agcgttgttc cagcggcagc tccagcagcc cggcgcgcag gcccatcgtc gccggcagga
     1201 agatgcgcgc atccatcagt cgcggcggct gcgggatcag cggcgcgaag tccatgtgcg
     1261 cgaggatgtc gcgctcgatg tcgatgccgg gggcgacctc ggtcagcacc agcccgcccg
     1321 cgctcagctc gaacacgcag cgctcggtca cgtacagcgc cttctgcccg cgcgcggcgg
     1381 cgaccggacc gctgtaggtg cggtgctcga cggcgcgcac gaacttgcgc tcgcggcctt
     1441 cctgctcgat gtgcagctgc ccgttcccga tcgcgagcct gaggccgccg gccgtgaagg
     1501 tgccgacgaa caccacgcgc ttggcgttct ggctgatgtt gatgaagccg ccggccccgg
     1561 ccaggcgcgg tccgaacttg ctgacgttca ggttgccctc ggcatcggcc tgcgcgaggc
     1621 cgaggaaggc gatgtcgagc ccgccgccgt cgtagaagtc gaactgcgag ggctggtcga
     1681 tgatcgcttg ggtgttgacc gccgccccga agttcaggcc cgaggccggc aggccgccga
     1741 tcacgccggg ctcggtggtc agcgtcagca gttcgagcac ctgttcctcg ttggcgacgc
     1801 cggcgatgcc ctcgggcatg ccgatgccga ggttgacgac ggcgttcggt cgcagctcga
     1861 aggcggcacg gcgcgcgatc agcttgcgct cgttgagcgc gagtggcgcg atgccggtca
     1921 ccggcacgcg gatctcgccc gagaacgccg ggctgtaggg ctcggcgaag gtctgctggt
     1981 gctcgcgcgc ctcggcgacc accacgcagt cgacgaggat gcccggaatc ttgacctggc
     2041 gtgccgacag cgaaccgcgc tcggcgatgc gctcgacctg cacgatgacg atgccgccgc
     2101 tgttgcgcgt cgcggtcgcg agcgccagcg cttcgagcgt cagcgcctcg cgctccatcg
     2161 tgatgttgcc gtccgggtca ccggtcgtgc cgcgcaggaa gcaggcgttg atcgggaagg
     2221 ccttgtagaa caggtagtcc tcgccgtcga tcggcaggcg ccggaccagc tcctcggtgg
     2281 tgcgctcgtt cagcttgccg ccgccgaact cggggtcgac gaaggtgccg agcccgacgc
     2341 gggtcagcgt gccgggcttg ccggcggcga tgtcgcggaa caggtgcgtg atcacgccct
     2401 gcggcaggtt ccaggcgctg atgcggccgt cgatcgcgag cttctgcagc ttcggcacca
     2461 gtccccagtg cccgccgacc acggagtcga ccagcccctc gtggccgaga tgattcaggc
     2521 cgcgctcggc gccgtcgccc tgcccggccg cgtacacgag ccgcaggttg cgcggctcgc
     2581 cggtttcgag aaagcgccgt tccagcgcac aggcgaggcc ttcggcaaag ccgatgccga
     2641 cgaagccgcc ggtgccgagc gtgtcgccgt cgcggatcag gcgcacggcc tcggcggcgg
     2701 tgaccacctt gcccttcggg gccagcggct ggccggcgag cagcggatgc ggatgtggac
     2761 ccatggatgc cctctccttc cctttggaat gatgccggct ggtgcggcaa tgtattggat
     2821 gtggagcggc cggatgccgc tccgcagtca gtggaggccg tcgccggacc tcagtcggcc
     2881 gtatgcagga ccagcacgtc gcagaccgcc gcaccgacca cccgttcggc cgtgccggca
     2941 accagccagt ccagccagtg gcggtcgtga tggccgacga tgatcaggtc gacgcccgcg
     3001 gcacgcgcct cggcgatgat cgccgtgctg accgaggtcg aggccacgac ccgcagctgc
     3061 gcacccggca cgccgaggcg ctgcgccagc gactgcagga agcgtgcggc ggcctcgtcg
     3121 agccgggttt cggtgtcgga ttccagcggc aggttttcct cggtgccctg gggcacgacc
     3181 agccggtgcg gctcgggcgt gccggcgacc gtcggcagca gctcgctgcc gccttcgctg
     3241 cgctcgtcga cgacgtgcag cagggtcagg cgcggggcgc cgtagcgcag cagttcgctg
     3301 acccgcgcca gcatccgcgg gccgaccgca tcgaattccg ctgcaagcag gacatgtcgg
     3361 taggtcatgg ctgtctccgc ctccgtc
//
LOCUS       DAOQIE010000378         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_18375_length_3343_cov_3.170012, whole genome shotgun sequence.
ACCESSION   DAOQIE010000378 DAOQIE010000000
VERSION     DAOQIE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18375_length_3343_cov_3.170012"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1985
                     /locus_tag="PK144_17030"
     CDS             <1..1985
                     /locus_tag="PK144_17030"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNF67476.1"
                     /translation="DAVNANRFTVAGLHAAIGATLDLLASPAGLLIKPLLTRDPTGEL
                     LRLLDELASRQQPARRAGVWSTADGSEALLLATTHAAGSDTDAQAAALATLQAAWADS
                     RPAAGLALQMSGPGVFAVQARATIKSEVERLSTLATVLIVALLWAVYRSPRLLVLGLL
                     PMLSGALAGVAAVGLGFGVVHGITLGFGITLIGEAVDYAIYLFVQSGAHVRADTGSWW
                     PTVRLGVATSVVGFATLLGAGFPGLAQLGTFSLAGLLVAALVTRHVLPVLMPAHWRLR
                     DITPFGERLAGLQRRLRRLAPLLVCLPLASALLLMLRGAGLWQHDLSALSPVPLADQQ
                     LDARLRAGLGAPDVRALIVIDAADAGSALSGAEALAPALDALVAGGRLGGYESPARYL
                     PSPGRQRARLAALPPADTLRTRLGEALAGLPLRAERLDDFIAEVEAARSRGLLTRADL
                     DGSSFALAVDALLLRQPDGRWHALLPLRAAADGTLPQRDTLATVLAQAGPGVQASHVD
                     LKREADALYAGYLAEAQALTLGGCAAIVLLLFAALRSVRRSLRVLAPLAGAMLTVTAA
                     LVLAGETLTLLHLVGLLLVVAVGSNYALFFVQASPAPATLVSLLLANLTTLLGFGLLG
                     FSSVPVLHALGVVVGPGALLALLYAAAFAPTRGAAP"
     gene            1982..2632
                     /locus_tag="PK144_17035"
     CDS             1982..2632
                     /locus_tag="PK144_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67477.1"
                     /translation="MNRALPVEYRPAVPGRHARALVVLLAAAGDDPQALAAGCAPPPG
                     DGRDYLYVESALDGLGDGSLLTSLHGQWLAPLRDRHAHLLLGGLSIGGLLALVLADAQ
                     PALADAHLLIAPYPGNRALVAGLRAAGGPGPWLALPAPETLERRGWRGLQGLCAAGRP
                     PWLGYGRADRYADGLQLMADDLPAARVRAIDGGHDWPTWRTLWALACDPPAMAIPS"
     gene            2629..>3343
                     /locus_tag="PK144_17040"
     CDS             2629..>3343
                     /locus_tag="PK144_17040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF67478.1"
                     /translation="MNPRRWHPSPLIAGSALLHVAVAGLLAARPGDWPLPLGLLAADH
                     LLLSAQGLWPRSRGLGPNWTRLPAAAAADGAVALTIDDGPDPELTPRVLDLLDAAGAR
                     ASFFCIGARARRHPRLIAEIVRRGHAVENHSEHHRHTFSLLGPRAMLREIATAQATLA
                     ERAGEAPRFFRAPAGLRNPWLDPILHRLDLRLASWTRRGFDTRERNPARVRTRLLRDL
                     DAGDILLLHDGHAAHTAGGE"
BASE COUNT          336 a         1288 c         1209 g          510 t
ORIGIN      
        1 gcgatgcagt caacgcaaac cgcttcacgg tcgcgggcct gcacgccgcc atcggcgcga
       61 cgctggacct gctcgcctcg ccggccgggc tgctgatcaa gcccctgctc acccgcgatc
      121 cgaccggcga gctgctgcga ctgctggacg agcttgccag ccggcagcag ccggcacggc
      181 gtgcaggcgt ctggagcacg gccgatggca gcgaggccct gctgctcgcc accacgcacg
      241 cggccggcag cgacaccgac gcacaggcgg ccgcgctcgc gacgctgcag gcggcctggg
      301 cggacagccg gccggccgcc ggtctcgcgc tgcagatgag cggccccggc gtgttcgcgg
      361 tgcaggcgcg tgccacgatc aagtccgagg tcgaacgcct gtcgacgctc gccaccgtgc
      421 tgatcgtcgc gctgctgtgg gccgtctacc gctcgccccg gctgctggtg ctcgggctgt
      481 tgccgatgct cagcggcgca ctggccggcg tcgccgcggt cggtctcggc ttcggcgtcg
      541 tgcatggcat cacgctcggt ttcggcatca cgctgatcgg tgaggccgtc gattatgcga
      601 tctacctgtt cgtgcaatcc ggcgctcatg tgcgcgccga caccggcagc tggtggccga
      661 ccgtccggct cggggtcgcg acctcggtgg tcggcttcgc gacgctgctc ggcgccgggt
      721 tcccgggact tgcacagctc ggcaccttct cgctggcggg gctgctggtc gccgcgctgg
      781 tgacgcgcca cgtgctgccg gtcctgatgc cggcgcactg gcgcctgcgt gatatcaccc
      841 cgttcggcga aaggctggcc gggttgcagc gccggctgcg ccggctggcg ccgctgctgg
      901 tctgcctgcc gctcgcctcg gcgctgctgc tgatgctgcg cggtgccggg ctctggcagc
      961 acgacctgtc ggcgctgagt ccggtgccgc tcgccgatca gcagctcgat gcccggctgc
     1021 gcgccggact cggcgcgccc gacgtgcgcg ccctgatcgt catcgacgcg gccgatgccg
     1081 gcagcgccct ctccggggcc gaagccctgg ctccggccct cgacgcgctg gtcgcaggcg
     1141 gccggctcgg cggctacgag agtccggcgc gctacctgcc gagtcccggg cgccagcggg
     1201 cccggctcgc ggcgctgccg ccggccgaca cgctgcgcac ccggctcggc gaggcactcg
     1261 ccgggctgcc gctgcgcgcc gaacgcctgg atgacttcat cgccgaggtc gaggccgcgc
     1321 gcagccgggg gctgctgacc cgtgccgacc tcgacggtag cagcttcgcg ctggccgtcg
     1381 atgcgctgct gctgcgccag cccgatggcc gctggcatgc cttgctgccg ttgcgcgccg
     1441 ctgccgacgg cacgctgccg cagcgcgaca cgctggccac ggtgctggcg caggccggcc
     1501 ccggcgtgca ggccagccat gtcgatctca agcgcgaggc cgatgcgctg tacgccggct
     1561 atctcgccga ggcgcaggcc ctgaccctcg gcggctgcgc ggcgatcgtg ctgctgctgt
     1621 tcgccgcgct gcgttcggtc cgccgcagcc tgcgtgtgct tgcgccactc gcgggcgcga
     1681 tgctgaccgt cacggcggca ctggtgctgg ccggcgagac gctgaccctg ctgcatctgg
     1741 tcggcctgct gctggtggtc gcggtcggct cgaactacgc actgttcttc gtgcaggcgt
     1801 cgccggcacc ggcaacgctg gtgtcgctgc tgctcgcgaa cctgacgacg ctgctcggct
     1861 tcggcctgct cgggttctcc agcgtgccgg tcctgcatgc gctcggtgtc gtcgtcggcc
     1921 ccggcgcgct gctggcgctg ctgtacgcgg ccgccttcgc gccgacccgg ggtgcggcgc
     1981 catgaaccgt gcgctgccgg tcgaataccg ccctgccgtc cccggccggc acgcccgtgc
     2041 actggtcgtg ctgctcgccg ccgccggcga tgacccgcag gcactggccg ccggctgcgc
     2101 ccccccgccc ggtgacgggc gtgattacct gtacgtggaa agtgccctcg acggcctcgg
     2161 cgacggctcg ctgctcacca gcctgcacgg gcaatggctg gccccgctgc gcgaccgcca
     2221 tgcgcatctg ctgctcgggg gcctgtcgat cggcggcctg ctggcgctgg tgctggcgga
     2281 tgcgcagccg gccctcgcgg acgcgcatct gctgatcgcc ccgtacccgg gcaaccgtgc
     2341 gctggttgcc ggtctgcgtg cagccggcgg tcccgggccc tggctggcgc tgcccgcccc
     2401 cgaaaccctc gaacggcgcg gctggcgcgg gctgcagggg ctgtgcgcgg ctggtcgccc
     2461 tccctggctc ggttacggcc gcgccgaccg ctatgccgac gggctgcagc tgatggccga
     2521 cgacctgcca gccgcacgcg tgcgggccat tgacggcggg cacgactggc cgacctggcg
     2581 cacgctgtgg gcgctcgcct gcgatccgcc cgccatggcg attccgtcat gaacccccgg
     2641 cgctggcacc cctcgccgct gatcgccggc tcggcgctgc tgcacgtcgc ggtggccggc
     2701 ctgctcgcgg cccgccccgg cgactggccg ctgccgctcg ggctgcttgc ggccgatcac
     2761 ctgctgctga gcgcgcaagg tctctggccg cgcagccgcg ggctcggtcc gaactggacg
     2821 cgactgccgg ccgcggccgc ggccgacggc gcggtggcac tgaccatcga cgacgggccc
     2881 gacccggagc tgacgccgcg cgtgctcgac ctgctcgatg ccgccggcgc gcgcgcgagc
     2941 ttcttctgca tcggcgcgcg tgcgcgccgc catccgcggc tgatcgccga gatcgtgcgc
     3001 cgcggccacg cggtcgagaa ccatagcgag catcaccggc acacgttctc gctgctcggt
     3061 ccgcgggcga tgctgcgcga gatcgccacg gcccaggcga cgctcgccga acgcgccggc
     3121 gaggcgcccc gctttttccg cgccccggcc ggcctgcgca atccctggct ggacccgatc
     3181 ctgcaccggc tcgacctgcg cctggcgagc tggacgcggc gcggcttcga cacccgcgag
     3241 cgcaatccgg cgcgtgtgcg cacccggctg ttgcgtgatc tcgatgccgg cgacatcctg
     3301 ctgctgcacg acggccatgc cgcgcacacg gccggtggcg aag
//
LOCUS       DAOQIE010000379         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_18741_length_3301_cov_10.518792, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000379 DAOQIE010000000
VERSION     DAOQIE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18741_length_3301_cov_10.518792"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PK144_17045"
     CDS             complement(<1..1741)
                     /locus_tag="PK144_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNF67479.1"
                     /translation="MFRRLSCCFALLLAVGGLAGATAQAAESEAVRSPRATVTLVADH
                     DTVAPGQRLDFGLRQRLAPHWHTYWKNPGDAGSPPQIELQLPEGASAGPIRWPGPERM
                     PVGPAQSFGYSDAIVLPFTVTVPAAARPGTRFTVEAEADWVVCEKECIPEQGRFRLEL
                     PVATTPVPAGGEVADSFRAAEARMPQAPAWPARLQAEGGALLLEVDGAASGGTIKDAF
                     WFPAAWGLIDHAAVQAVSADARTGRLQLALVRGQAFSPQAALDGVLALRRTSGTTSWY
                     ALTPAHAAAAAASDAPAAAAGFAAWQAALYAFAGGLLLNLMPCVFPVLAIKAGALARL
                     AGHGASAVRRSALAYTAGVLAALLALAGTLLALRAGGAALGWGFQFQSPPFVVLTLWL
                     LFATGLNLSGVFEVGAGLAGRGQSLAGRPGHAGSFFTGLLAVVVATPCTAPFMAAALG
                     AALAAPVPVALAIFVALGLGLALPYLLLALVPALAQRLPRPGAWMQRLREVLAFPMYA
                     AAAWLLWVLAQLAGADALPVALGGALLIAFAGWLGGRAQSAGDGRGARLAHGGALAAV
                     LAAALLLPLLPAAT"
     gene            2036..2755
                     /locus_tag="PK144_17050"
     CDS             2036..2755
                     /locus_tag="PK144_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNF67480.1"
                     /translation="MSHNHSGHHDAGTQPCAICGKPFARRDLVPAALVREPLQQLLQR
                     ERPDWNAQAWICRPDLAELRARYVHSVLEAEKGELTGLEQAVLDSLRRHEILSANVDA
                     EQEQAWSFGERLADRIASFGGSWSFLILFMLFIAVWIAMNSLVMFFRPLDPFPYILLN
                     LLLSCLAAVQAPIIMMSQNRQEARDRVRAEHDYQVNLKAELEIRHLHEKIDHLLNHQW
                     ERLLQIQEVQVDLLTELRRPR"
     gene            2923..>3301
                     /gene="trfA"
                     /locus_tag="PK144_17055"
     CDS             2923..>3301
                     /gene="trfA"
                     /locus_tag="PK144_17055"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HNF67481.1"
                     /translation="MNDPIGKARELAARQRQKTAASATVSDKPAGEQLPLWSEALRGV
                     PNELVRSSLFSARDKREPRRSFRGEDLVVLGDGRINYRGEELRQDDETVWLHVLHLAR
                     LHPLGESVEFTPHAFLKAIGWGTS"
BASE COUNT          505 a         1202 c         1192 g          402 t
ORIGIN      
        1 gcgtcgccgc gggcagcagc ggcagcagca gcgccgccgc gagcacggcc gccagggcgc
       61 cgccatgcgc aagacgcgcc ccgcgcccgt cgccggcgga ctgcgcgcga ccgcccagcc
      121 agccggcgaa cgcgatcagc agcgctccgc cgagcgcgac cggcagcgca tcggcaccgg
      181 cgagctgcgc gagcacccac agcagccagg ccgcagcggc gtacatcggg aaggccagca
      241 cctcgcgcag ccgctgcatc cacgctcccg gccgcggcag gcgctgtgcg agcgccggca
      301 cgagcgcgag cagcagatac ggcagcgcca gcccgaggcc gagcgcgacg aagatcgcga
      361 gcgccaccgg caccggcgcg gcgagtgccg ccccgagcgc ggcggccatg aacggcgccg
      421 tgcacggcgt ggcgacgacg acggcgagca ggccggtgaa gaagctgccg gcgtgtccgg
      481 gccgcccggc gagcgactgg ccacggccgg cgagtccggc gccgacttcg aacacgccgg
      541 agagattcag cccggtcgcg aacagcagcc agagcgtcag caccacgaac ggcggcgact
      601 ggaactggaa gccccagccg agcgcggccc cgccggcgcg cagcgcgagc agcgtgccgg
      661 cgagcgcgag cagggccgcc agtacaccgg ccgtgtacgc gagcgccgac cgccgcaccg
      721 cgctcgcgcc gtgcccggcg agccgtgcga gcgcgccggc cttgatcgcc agcaccggga
      781 acacgcaggg catcaggttc agcagcaggc cgccggcgaa ggcgtagagc gccgcctgcc
      841 aggccgcaaa ccctgcggcc gctgccgggg catcgctcgc cgcggccgct gcggcatggg
      901 ccggtgtcag cgcataccag ctcgtggtgc ctgaggtgcg gcgcagggcc agcacgccgt
      961 cgagcgccgc ctgcggcgaa aacgcctgtc cgcgcaccag cgccagctgc agccgcccgg
     1021 tccgcgcatc ggcgctgacg gcctgcacgg cggcgtgatc gatcaggccc caggctgccg
     1081 gaaaccagaa ggcgtccttg atcgtgccgc cgctcgccgc gccatcgact tcgagcagca
     1141 gcgcgccgcc ctcggcctgc agccgcgccg gccacgcagg cgcctgcggc atgcgcgctt
     1201 cggcggcgcg gaagctgtcg gcgacctcgc cgccggccgg tacgggtgtc gtcgcgaccg
     1261 gcagctccag ccggaagcgc ccctgctcgg ggatgcattc cttctcgcac accacccagt
     1321 cggcttcggc ctcgaccgtg aagcgcgtgc cgggccgggc cgcggccggt accgtcaccg
     1381 tgaacggcag cacgatcgca tccgagtagc cgaagctctg cgccggaccg accggcatgc
     1441 gctcgggacc gggccagcgg atcggcccgg cactcgcgcc ttcgggcagc tgcagctcga
     1501 tctgcggcgg cgagccggca tcaccggggt tcttccagta ggtatgccag tgcggcgcga
     1561 ggcgctggcg caggccgaag tcgagcctct ggcccggggc gaccgtgtcg tgatcggcga
     1621 ccagcgtcac cgtcgcgcgc ggcgagcgca cggcttcgct ctcggcggcc tgcgcggtgg
     1681 cgcccgccag tccgccgaca gcgagcagca gcgcgaagca gcaggacagg cgtcggaaca
     1741 tcggcatgga ctccacggac gggacgacgg cgggcatacg gactcgcatg gccgggcgcc
     1801 ggttccggcg gttacacgaa aaagttccgt gcccggcaaa gtatttttcg cagccgttac
     1861 agggtttctg ccgcgaaatc cccatcgctg gccgctacgg cgcgctgcgc ccggcaggat
     1921 gcgcacggat gcgtgtcaca ccgatgcatc cctgcatgcc ttgcgggcgt gcgctgtgcc
     1981 ggcggcgtac gctgccggcc ttcaccccgg attcgacccg aaaggaaggc tgccgatgtc
     2041 ccacaaccac tcaggccatc acgacgcggg cacgcagccc tgcgcgatct gcggcaagcc
     2101 gttcgcgcgg cgcgatctgg tgccggcggc gctggtgcgc gagccgctgc agcagctgct
     2161 gcagcgcgag cggccggact ggaacgcgca ggcctggatc tgccggcccg atctggccga
     2221 gctgcgcgcg cgctacgtgc attcggtgct cgaagccgag aagggcgagc tgaccgggct
     2281 ggagcaggcg gtgctcgaca gcctgcgccg gcacgagatc ctgtcggcca acgtcgatgc
     2341 cgagcaggag caggcctgga gcttcggcga gcggctcgcc gatcgcatcg cgagcttcgg
     2401 cggcagctgg agcttcctga tcctgttcat gctgttcatc gccgtctgga tcgcgatgaa
     2461 ctcgctggtg atgttcttcc ggccgctcga cccgttcccg tacatcctgc tgaacctgct
     2521 gctgtcgtgc ctcgcagccg tgcaggcgcc gatcatcatg atgagccaga accgtcagga
     2581 ggcccgcgac cgcgtgcgcg ccgagcacga ctatcaggtc aacctgaagg ccgagctgga
     2641 gatccgccac ctgcacgaga agatcgacca cctgctgaac caccagtggg agcggctgct
     2701 gcagatccag gaggtgcagg tcgatctgct gaccgagctg cgccggccgc ggtaaaaatc
     2761 cgcagcgtgc cgtgccggtt ccggcttgca ggccgggttg agccgcccgg atggatggca
     2821 tcaagcgatg cgctggcctg aatcgatccg ctcgtgcagc ctgccggcgt ccggccgacc
     2881 gcccgggcgg cggcgacggc ttgcggaggc cctgcagacc ccatgaacga cccgatcgga
     2941 aaggcgcgcg aactcgccgc ccgccagcgc cagaagactg ccgccagcgc cacggtgagc
     3001 gacaagccgg ccggtgagca gctgccgctg tggtcggagg cgctgcgcgg cgtgccgaac
     3061 gagctcgtgc gctcctcgct gttctcggct cgcgacaagc gcgagccgcg gcgcagcttc
     3121 cgcggtgagg atctggtcgt gctcggcgat gggcgcatca actaccgcgg cgaggaactg
     3181 cgccaggacg acgaaaccgt ctggctgcat gtgctgcatc tggcgcgcct gcatccgctc
     3241 ggcgagtcgg tcgagttcac gccgcacgcc ttcctgaagg cgatcggctg gggcacgagt
     3301 g
//
LOCUS       DAOQIE010000380         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_18972_length_3276_cov_8.149333, whole genome shotgun sequence.
ACCESSION   DAOQIE010000380 DAOQIE010000000
VERSION     DAOQIE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18972_length_3276_cov_8.149333"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK144_17060"
     CDS             <1..370
                     /locus_tag="PK144_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67482.1"
                     /translation="KQTAQATQEIAAQIGGIQTATDGTVGAIGTISSVIQTVNDIATA
                     IAAAVEQQSAATREIARNVQLTADSAMTVSRHIGEVTHAATDTGSQSEELLEASRQLS
                     RQSEDLRREVDRFISAIRSE"
     gene            640..3249
                     /locus_tag="PK144_17065"
     CDS             640..3249
                     /locus_tag="PK144_17065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67483.1"
                     /translation="MRKNLFIVLLVVAANLGIWAAFNRPIAEEPWHGMINGFSYSPYG
                     RDDNPLESRMPKPEAIDRDLALLAQYGRSVRTYSAIDGLEVIPALAQKHGLQVTLGAW
                     LDQRTELNKREIDSLIDLGKRYDNVTRLVVGNEAILRADLTVRELAQYLRQVRKAVNK
                     PVSTAEPWHVWLRFPELVQEVDFIAVQLLPYWEKVPFEQAVEYVTRRYNELRTAYPDK
                     PILISEVGWPSEGNRVGAAVPSLAHEARFIRRFLNEASENGMDYFVMEAFDQPWKKGI
                     EGSVGAYWGVFSASREPKFPMSGLIEEDASWNSQAVLAACVALLPMVLFGLRFRNLRA
                     HGQLFFAVLIQASASLLVWTLFVPAHLYLTDLGLLLLGILLPAQIGLLLVALINGFEF
                     TEVLWKQDWKRAFKPLTGHEARTDWPMVSLHLACYNEPPELVIETLNSLAALDYPNFE
                     VLVIDNNTPDEAVWRPLEAHCARLGPHFRFMHLKPWKGFKAGALNFGLRRTHPSARIV
                     GVIDADYLVTPDWLRSLVPYFDDPKVGFVQAPQDHRDWQHDRFTEWANWEYSGFFNIG
                     MVHRNERDAIIQHGTMTLIRKAAIDASGPWAEWCICEDSELGLRVMEEGYEAVYVNHA
                     FGKGVAPDTFGAYKGQRFRWAYGAVQILKRYWKWMLPNFLGGTGRLSASQKFHFVTGW
                     LPWFADALHLVFTISAMLWSVGLLLLPQYFEFPLAVFLFPTLGMFAFKIMHSFVLYTA
                     RVPCSLSQRLGAAVAGMGLTHSIARAIFAGLFTKGRAFLRTPKGENSAALVRGLLMAR
                     EEGLILLALWTCAFGVGLEYGLHVREVTFWIVILLVQSIPYAAAVLHSIASALPGGTR
                     DSEPAAPAALAAG"
BASE COUNT          507 a         1177 c         1081 g          511 t
ORIGIN      
        1 caagcagacc gcgcaggcga cccaggagat cgccgcgcag atcggcggca tccagaccgc
       61 caccgacggc acggtcggcg cgatcggcac gatcagcagc gtgatccaga ccgtcaacga
      121 catcgccacc gcgatcgccg ccgccgtcga gcagcagagc gccgccaccc gcgagatcgc
      181 ccgcaacgtg cagctgaccg ccgacagcgc gatgacggtc agccgccaca tcggcgaggt
      241 cacgcacgcg gccaccgaca ccggcagcca gtccgaggaa ctgctggaag cctcgcgcca
      301 gctgagccgc cagtccgagg acctgcgccg cgaggtcgac cgcttcatca gcgcgatccg
      361 cagcgagtga tgccgatcac aggggattgc atgatttcct ctccccaccc cctcccccgc
      421 gagggacgag ctgaaaagcg cagcgctctg gtgtgagcgg ttgagcccgc cgccgggccg
      481 gccggccgcc ggctcgacag cagcggcggc cccttcctag aatcccccgc cgccgccgcg
      541 ccccttgcgc ggcgaacgtc ccgcctcgcg tcgcggtccg aacccgcggc cttccgctgc
      601 ctccgccctc ccctccgaac cgtcccgaag gttccgcgca tgcgcaagaa tctgttcatc
      661 gtcctgctgg tcgtggccgc caacctcggc atctgggcgg cgttcaaccg tccgatcgcc
      721 gaggaaccct ggcacggcat gatcaacggc ttctcgtact cgccgtacgg gcgcgacgac
      781 aacccgctcg aaagccgcat gccgaagccc gaggcgatcg accgcgatct cgcgctgctc
      841 gcgcagtacg ggcgcagcgt gcgcacgtat tcggcgatcg acggcctgga ggtgattccg
      901 gcgctggcgc agaagcacgg cctgcaggtc acgctcggcg cctggctcga ccagcgcacc
      961 gagctgaaca agcgcgagat cgacagcctc atcgacctcg gcaagcgcta cgacaacgtc
     1021 acgcgcctcg tcgtcggcaa cgaggcgatc ctgcgcgccg acctgaccgt gcgcgaactc
     1081 gcgcagtacc tgcgtcaggt gcgcaaggcc gtcaacaagc cggtatcgac cgccgagccc
     1141 tggcacgtgt ggctgcgctt cccggagctg gtgcaggagg tcgacttcat cgccgtgcag
     1201 ctgctgccgt actgggaaaa ggtgcccttc gagcaggcgg tcgaatacgt gacgcgccgc
     1261 tacaacgagc tgcgcaccgc ctatccggac aagccgatcc tgatctccga ggtcggctgg
     1321 ccgagcgagg gcaaccgcgt cggtgccgcc gtgccgagcc tcgcgcacga ggcgcgcttc
     1381 atccgccgct tcctgaacga ggcgagcgag aacggcatgg attacttcgt catggaagcc
     1441 ttcgaccagc cgtggaagaa gggcatcgag ggctcggtcg gcgcctactg gggcgtgttc
     1501 agcgccagcc gcgagccgaa gttcccgatg agcgggctga tcgaggagga cgcgagctgg
     1561 aactcgcagg ccgtgctcgc cgcctgcgtc gcgctgctgc cgatggtgtt gttcgggctg
     1621 cgcttccgca acctgcgcgc ccacggccag ctgttcttcg cggtgctgat ccaggcgagc
     1681 gcctcgctgc tggtgtggac gctgttcgtg cccgcgcacc tgtacctgac cgacctcggc
     1741 ctgctgctgc tcggcatcct gctgccggcg cagatcggcc tgctgctggt ggcgctgatc
     1801 aacggcttcg agttcaccga ggtgctgtgg aagcaggact ggaagcgcgc gttcaagccg
     1861 ctgaccggcc atgaggcgcg caccgactgg ccgatggtgt cgctgcacct cgcctgctac
     1921 aacgagccgc ccgagctggt catcgagacc ctcaacagcc tcgccgcgct cgattacccg
     1981 aacttcgaag tgctggtcat cgacaacaac acgccggacg aggccgtctg gcggccgctc
     2041 gaagcgcact gcgccagact cggcccgcac ttccgcttca tgcacctgaa gccctggaag
     2101 ggcttcaagg ccggggcgct gaacttcggc ctccggcgca cgcacccgtc ggcgaggatc
     2161 gtcggcgtca tcgatgccga ctatctggtc acgcccgact ggctgcgctc gctggtgccg
     2221 tacttcgacg atccgaaggt cggcttcgtg caggcgccgc aggatcaccg cgactggcag
     2281 cacgaccgct tcacggagtg ggcgaactgg gagtactcgg gcttcttcaa catcggcatg
     2341 gtgcatcgca acgagcgcga cgcgatcatc cagcacggca cgatgacgct gatccgcaag
     2401 gccgcgatcg acgcctccgg cccctgggcc gaatggtgca tctgcgagga ctccgaactc
     2461 ggcctgcgcg tcatggagga aggctacgag gcggtctacg tcaaccacgc cttcggcaag
     2521 ggcgtcgcgc ccgacacctt cggcgcctac aagggccagc gcttccgctg ggcctacggt
     2581 gcggtgcaga tcctcaagcg ctactggaag tggatgctgc cgaacttcct cggcggcacc
     2641 gggcggctga gcgcctcgca gaagttccac ttcgtgaccg gctggctgcc gtggttcgcc
     2701 gatgcgctgc atctggtgtt cacgatctcg gcgatgctgt ggtcggtcgg cctgctgctg
     2761 ctgccgcagt acttcgagtt cccgctggcg gtgttcctgt tcccgacgct cggcatgttc
     2821 gcgttcaaga tcatgcacag cttcgtgctc tacaccgcgc gcgtgccgtg ctcgctgtcg
     2881 cagcgcctcg gcgcggcggt cgccggcatg ggcctgacgc actcgatcgc acgcgcgatc
     2941 ttcgccggcc tgttcaccaa gggccgcgcc ttcctgcgca cgccgaaggg cgagaacagc
     3001 gcggcgctgg tgcgcggcct gctgatggcg cgcgaggagg gcctgatcct gctggcactg
     3061 tggacctgcg ccttcggcgt cggcctcgaa tacggcctgc acgtgcgcga ggtgacgttc
     3121 tggatcgtga tcctgctcgt gcagtcgatc ccgtacgcgg cggccgtgct gcactcgatc
     3181 gcgagcgcgc tgcccggcgg cacgcgcgac agcgaaccgg ccgccccggc cgcgctcgcc
     3241 gccggctgat gccggccgcg cggcctccgg gcgcag
//
LOCUS       DAOQIE010000381         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19069_length_3267_cov_8.834682, whole genome shotgun sequence.
ACCESSION   DAOQIE010000381 DAOQIE010000000
VERSION     DAOQIE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19069_length_3267_cov_8.834682"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PK144_17070"
     CDS             <1..1067
                     /locus_tag="PK144_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAF repeat-containing protein"
                     /protein_id="HNF67484.1"
                     /translation="AALFGFHAASGAAPLYAVIDLGTLGGGSSQALGLNNAGQISGIA
                     ALTNNAGANAFLYSGGVMTDLGTLGGATSNGQDINAAGQVVGSSLTAGNSDTHAFLYS
                     GGTMTDLGTLGGASSGAAAINNLGQVVGFSQIAGNLQSHAFFFDGTTMNDLGTLGGTT
                     SVALDLDDSGRIVGQSFDSNGDAHAFLYEAGLMNDLGTLGGTTSTAAAINAGGLIAGY
                     GQITGDTGTHPFLYSGGVMTDLGTLGGDSDFLVALNAAGQGVGMSFLPGAAFPVGLFW
                     DGGALRDLNALIDPASGWTIRGATDINDAGWIVGYGNDGGIDTRALLLVPLAAIPEPD
                     LLTLIGAGAWLLGRRRRETT"
     gene            1375..1647
                     /locus_tag="PK144_17075"
     CDS             1375..1647
                     /locus_tag="PK144_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67485.1"
                     /translation="MLSLQDCCDFCSLSEDEVAAIAEHEHLPEVAAAGCGQELLQSAA
                     GIFRLHDFIRDDIGVARVHGDTAHACHLEAVLSGFEHSHPESLRLH"
     gene            complement(1667..2782)
                     /locus_tag="PK144_17080"
     CDS             complement(1667..2782)
                     /locus_tag="PK144_17080"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNF67486.1"
                     /translation="MSEPVDPSGLTVGIVGGGQLARMLALAGYPLGLHFIVLDPAKDA
                     CAGQVATQLHGEYDDPDALDELARRCDVVTFDFENVPAAALEHLAAEVPVAPNPRALG
                     TAQDRLAEKTLFRELDIPTPVYAAVDSREELSIAVATTGLPAVLKTRRFGYDGKGQRV
                     LRERADLDAAWAELAGHALILEGFVPFTRELSILAARSADGTTVFWPLVENVHTGGIL
                     RLSRAPADAEALEATARAHAQAVLDALDYVGVLAIEFFDVGGTLIANEMAPRVHNSGH
                     WTLEGAETSQFENHLRAVCGLPLGSTRAVGYSAMLNFIGRLPEPGPFLATPGLHWHDY
                     GKAPRSGRKVGHATLRTDQPDELEHALARLQPLLAAD"
     gene            complement(2779..>3267)
                     /gene="purE"
                     /locus_tag="PK144_17085"
     CDS             complement(2779..>3267)
                     /gene="purE"
                     /locus_tag="PK144_17085"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNF67487.1"
                     /translation="PLVGLIMGSRSDWSTLEHAAATLETLGVPHEVRVVSAHRTPDLL
                     FEYAGSAEARGLEVILAGAGGAAHLPGMVASKTVLPVLGIPVQSAALNGLDSLLSIVQ
                     MPGGIPVGTLAIGKAGAINAALLAASMLGGKYPAIREAVRAFRTRQTDTVLADPDPRL
                     PL"
BASE COUNT          478 a         1094 c         1207 g          488 t
ORIGIN      
        1 tggccgcgct gttcggattc cacgcggcga gcggggcggc accgctgtat gcggtgatcg
       61 atctcggcac gctcggcggc ggttcgtcgc aggccctcgg cctcaacaac gccgggcaga
      121 tcagcggcat tgccgcgctc accaacaacg ccggtgccaa cgccttcctc tacagcggcg
      181 gcgtgatgac ggatctcggc acgctcggcg gcgcgaccag caacggtcag gacatcaatg
      241 ccgccgggca ggtcgtcggt tcgtcgctga cggccggcaa cagcgacacg catgccttcc
      301 tctacagcgg cggcacgatg acggacctcg gcacgctcgg cggtgcgagc agcggcgcgg
      361 cggcgatcaa caacctcggg caggtggtcg gcttttcgca gatcgccggc aatctgcagt
      421 cgcatgcgtt cttcttcgac ggcacgacga tgaacgatct cggcacgctc ggcggcacga
      481 ccagcgtcgc gctcgatctc gatgacagcg gccgcatcgt cggccagtcc ttcgacagca
      541 atggcgatgc gcatgccttc ctgtacgagg ccgggctgat gaacgacctc ggcacgctcg
      601 gcggcacgac cagcaccgcc gcggcgatca atgccggcgg actcatcgcc ggctacgggc
      661 agatcaccgg cgacaccggc acgcatccgt tcctctacag cggcggcgtg atgacggatc
      721 tcggcacgct cggcggcgac agcgactttc tggtggcgct gaacgccgcc ggtcagggcg
      781 tcggcatgag cttcctgccg ggggcggcgt ttccggtggg cctgttctgg gacggcggag
      841 ccttgcgcga cctgaatgcg ctgatcgatc cggcctcggg ctggacgatc cggggagcga
      901 ccgacatcaa cgatgccggc tggatcgtcg gctacggcaa cgatggcggc atcgacaccc
      961 gcgcgctgct gctggtgccg ctggcggcga ttccggaacc ggacctgctg acgctgatcg
     1021 gggccggggc gtggctgctc gggcgccgcc ggcgggagac gacgtgaggg cggaggtcga
     1081 tcacgcttca atgcgcggtt tgcgcccgct ttccccaggc cctcacccgt gagcggagag
     1141 ggactgaaaa gacggtgtgt tcggccgtga tcggcatgag gccggggccg tacgtctgcc
     1201 cgcggccccg gcgggacggg gcgactgccg gccggggcct gcggcccgca ttgccatatg
     1261 gtcatgaaac ttcgcttgtg cccggtcaga ccacggcgca taagcgccgc tgcgcagcgt
     1321 ctgttccagc cccgctaccc cggggacggc aggctcttcg tcaggaagcc catcatgttg
     1381 tcactgcagg actgttgcga tttctgttcg ctttccgaag acgaagtggc ggccatcgcc
     1441 gagcacgaac atctgcccga ggtcgcggcg gccggctgcg ggcaggagct gctgcagtcg
     1501 gcggccggca tcttcaggct gcacgacttc atccgcgacg acatcggcgt cgcccgggtg
     1561 cacggcgata ccgcgcatgc ctgccatctc gaagcggtgc tgagcggctt cgagcacagt
     1621 cacccggaat cgctgcgcct gcactgagcg cccgccggcg ccatgttcag tcagcggcga
     1681 gcaggggctg cagccgcgcc agtgcgtgct cgagttcgtc cggctgatcg gtgcgcagcg
     1741 tggcgtggcc gaccttgcgc ccgctgcgcg gggccttgcc gtagtcgtgc cagtgcaggc
     1801 cgggcgtggc gagaaacggc cccggctcgg gcagccggcc gatgaagttc agcatcgcgc
     1861 tgtagccgac ggcgcgcgtc gaaccgagcg gcaggccgca gacggcgcgc aggtggttct
     1921 cgaactgcga ggtctcggcg ccttcgaggg tccagtgccc ggagttgtgc acgcgcggcg
     1981 ccatctcgtt ggcgatcagc gtgccgccga catcgaagaa ctcgatcgcc agcacgccga
     2041 cgtaatcgag cgcatcgagc acggcctgcg cgtgggcgcg ggccgtggct tcgagggctt
     2101 cggcgtcggc cggcgcgcgg gacaggcgca ggatgccgcc ggtgtggacg ttctcgacca
     2161 gcggccagaa cacggtcgtg ccgtcggcac tgcgcgcggc gaggatcgac agctcgcgcg
     2221 tgaacggcac gaagccttcg aggatcagcg catggccggc gagttcggcc caggcggcat
     2281 cgaggtcggc gcgctcgcgc agcacgcgct ggcccttgcc gtcgtagccg aagcggcgcg
     2341 tcttcagcac cgccggcagg ccggtggtgg cgacggcgat cgacagttcc tcgcggctgt
     2401 cgacggcggc atacaccggt gtcgggatgt cgagttcgcg gaacagggtc ttttcggcga
     2461 ggcggtcctg cgcggtgccg agcgcgcgcg ggttcggcgc gaccgggact tcggcggcga
     2521 gatgttcgag cgccgcggcc ggcacgttct cgaagtcgaa ggtgaccacg tcgcagcggc
     2581 gcgccagctc atcgagcgcg tccggatcgt cgtattcgcc gtgcagctgc gtggcgacct
     2641 gaccggcgca ggcatccttc gccggatcga gcacgatgaa gtgcaggccg agcggatagc
     2701 cggcgagcgc cagcatgcgg gcgagctggc caccgccgac gatgccgacc gtcaggccgg
     2761 aaggatcgac gggctcactc acagcggcag cctcgggtcg gggtcggcga gcacggtgtc
     2821 ggtctggcgg gtgcggaagg cgcgcacggc ctcgcggatc gccgggtact tgccgccgag
     2881 catcgacgcg gcgagcagcg cggcattgat cgcgccggcc ttgccgatcg ccagcgtgcc
     2941 gaccgggatg ccgcccggca tctgcacgat cgacagcagc gaatcgaggc cgttgagcgc
     3001 cgccgactgc accgggatgc cgagcaccgg cagcacggtc ttgctcgcga ccatgcccgg
     3061 caggtgcgcc gcgccgccgg cgccggcgag gatgacttcg agtccgcgtg cctcggcgct
     3121 gccggcgtat tcgaacagca ggtcgggcgt gcggtgcgcg gagacgacgc gcacctcatg
     3181 cgggacgccg agggtttcga gcgtcgcggc ggcgtgttcg agcgtgctcc agtcggacct
     3241 ggagcccatg atgagaccga cgagggg
//
LOCUS       DAOQIE010000382         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19103_length_3264_cov_7.519788, whole genome shotgun sequence.
ACCESSION   DAOQIE010000382 DAOQIE010000000
VERSION     DAOQIE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19103_length_3264_cov_7.519788"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            577..1956
                     /gene="radA"
                     /locus_tag="PK144_17090"
     CDS             577..1956
                     /gene="radA"
                     /locus_tag="PK144_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNF67488.1"
                     /translation="MPPKTARTVYVCDACGAVSPKWAGQCGECGAWNSLQETVAAPPA
                     ATAARGGRLAGYAGASETRVQNLAEVGTDEAARLATGIQELDRVLGGGLTVGSVVLIG
                     GDPGIGKSTLLLQTLAHLAAQHRALYVTGEESPQQVSLRAQRLGLPREPLRLLPETCI
                     ERVLAVAAAEQPRVMVIDSIQTAYTELLQSAPGAVAQVRESAAQLVRFAKRSGTALFL
                     VGHVTKEGTLAGPRVLEHMVDTVLYFEGDSGSRFRALRAVKNRFGPVNELGVFAMTET
                     GLKEVTNPSAIFLSRHPEPVAGSVVMVTREGTRPLLVEVQALVDDCHGGNPRRVTLGL
                     EQNRLAMLLAVLHRHAGIAMFDQDVYVNVVGGVRVQETASDLAVLMAALSSFRDRPLP
                     NDLIVFGEVGLAGEIRPVPNGEERLREAAKHGFRRAIVPKANVPRGRLPEGFSVQAVE
                     RLAEALAEV"
     gene            2117..2479
                     /locus_tag="PK144_17095"
     CDS             2117..2479
                     /locus_tag="PK144_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67489.1"
                     /translation="MSRFLLRWLIVLLLLPGPGLAAAPALAAGHGALDRLALPVVDAG
                     PVLTARPGPGLHHDSGACPDTPASGQAHERCCDGCPAVPGTLVGLAAPAVPLASRLPY
                     RLPADPGHVTGPLRRPPR"
     gene            2602..>3264
                     /locus_tag="PK144_17100"
     CDS             2602..>3264
                     /locus_tag="PK144_17100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF67490.1"
                     /translation="MSRRLHRTGGALALLCLPFTLLAAPADADLPGATLAGLIEWAET
                     RNPELAVAGHELDAAEAMVGSADALPDPSFSIEWRDIDADRLTLDPSRVGSTKYTFAQ
                     TFPLWGKRGLQADIARAEAERARRQRSSVSVELRARLSAAHAERWQVRELLRLNAEQR
                     SLLRDLERIATARYADGLTAQSDAIRAQTERTQLEAERVRLEAERRRLGAQLNALLGR
                     RPD"
BASE COUNT          385 a         1191 c         1164 g          524 t
ORIGIN      
        1 ttccgtggtc ccgcatccgg cgggggattc cctgaagctt ccgtgtgcct gccggctccg
       61 tccggtggcg tcgatgtcat cggcttcccg tgccgtgtcc gtccgtggtt cccgcatccg
      121 gcgggagatt ccctgtcact tccctgagcc tgccggtgtc cgtccggcgg ttccgtattg
      181 ctcggcttcc cgatgccgta tccatccgtg gtcccgcatt ccgctgcggg tattccctga
      241 acttccctgc tgcagccgtt tccgtcggtg cgagccagac cagtgattca agccttgaaa
      301 ccgtttccct gtcccgtccc tcgggaccgc gttcctcgcg gtggccttcc gtagtgcttc
      361 ctgctgcggg ctccggtcct tcggagcagt cctgcgtgat caggtccgga tgtgatgcat
      421 tgtgtcgatc cacccggggt atgcccatcg ggcggcagcg catcgtcggc tgcgaaaatt
      481 cgtaacattg ctgcgagcgc agggcgcggc ttgagtaccg cccggccggc cccgatcgtc
      541 cgtccgtatc ttccccacct gcccccggga gtcgccatgc cgccgaaaac cgcccgcacc
      601 gtctacgtct gcgatgcctg tggtgccgtg tcgccgaagt gggccgggca gtgcggcgag
      661 tgcggggcgt ggaacagcct gcaggagacc gtcgccgcgc cgccggccgc caccgccgcg
      721 cgcggcgggc ggctcgccgg ctatgccgga gcgagtgaga cccgcgtgca gaacctcgcc
      781 gaggtcggca ccgacgaggc cgcgcggctt gcgaccggca tccaggagct cgaccgcgtg
      841 ctcggcggcg ggctgacggt cggctcggtg gtgctgatcg gcggcgatcc cggcatcggc
      901 aagtcgacgc tgctgctgca gacgctcgcg catctggccg cgcagcaccg ggcgctgtac
      961 gtgaccggcg aggaatcgcc gcagcaggtc agcctgcgcg cgcagcgcct cggcctgccg
     1021 cgtgagccgc tgcgcctgct gcccgagacc tgcatcgaac gcgtgctcgc ggtggcggcg
     1081 gccgagcagc cgcgggtgat ggtcatcgac tcgatccaga ccgcgtacac cgagttgctg
     1141 cagtcggcgc ccggtgcggt cgcgcaggtg cgcgagagcg cggcgcagct cgtgcgcttc
     1201 gccaagcgca gcggcacggc gctgttcctg gtcgggcacg tgaccaagga gggcacgctt
     1261 gccgggccgc gcgtgctcga acacatggtc gacacggtgc tgtacttcga aggcgattcc
     1321 ggctcgcgct tccgggcgct gcgcgcggtc aagaaccgct tcgggccggt caacgaactc
     1381 ggcgtgttcg cgatgaccga gaccggcctg aaggaagtca cgaatccgtc ggcgattttc
     1441 ctgtcgcgcc accccgagcc ggtcgccggc tcggtggtca tggtcacgcg cgagggcacg
     1501 cggccgctgc tggtcgaggt gcaggcgctc gtcgatgact gccacggcgg caatccgcgg
     1561 cgcgtgacgc tcgggctgga gcagaaccgc ctggcgatgc tgctcgccgt gctgcaccgc
     1621 cacgccggca tcgcgatgtt cgatcaggat gtctacgtca acgtcgtcgg cggcgtgcgc
     1681 gtgcaggaga cggcttcgga cctcgccgtg ctgatggcgg cgctgtcgag cttccgcgac
     1741 cggccgctgc cgaatgacct gatcgtgttc ggcgaagtcg gcctcgccgg cgagatccgc
     1801 ccggtgccga acggcgagga gcgcctgcgc gaggccgcca agcacggctt ccggcgtgcg
     1861 atcgtgccga aggccaacgt gccgcgcggg cggctgccgg aggggttctc ggtgcaggcg
     1921 gtcgagcggc tggccgaggc gctggcggag gtatgaggtc cgtccccttg cgcggcctgt
     1981 actctcgggg ccgcgctgtg ccagcctttg cagccgctgc gccggcggct tgaccgccat
     2041 caacgacgag gcaccggcct gcgcgtcagg ctgttccggt tttccgctcc tgcccccccg
     2101 aaacttccgt acctccgtgt cccgattcct gctgcgatgg ctgatcgtgc tgctgctcct
     2161 gccggggccg gggctggcgg ctgccccggc gctggcggcc gggcacgggg cgctcgaccg
     2221 gctggcgctg ccggtggtcg atgcggggcc ggtgctcacg gcgcgtccgg ggccggggct
     2281 tcatcatgat tccggtgcct gtccggacac gcccgcgtcc ggacaggcgc acgaacgctg
     2341 ttgcgacggc tgtccggccg tgccgggcac gctggtcggg ctggccgcgc cggcggtgcc
     2401 gctcgcctcg cgcctgccgt accggctgcc ggccgatccc gggcacgtga ccgggccgct
     2461 gcgccggcct ccgcgctgat tcctcctcgc cacgcctgca ccgtgccgtc cgctgctgcg
     2521 gacggtgcct gccgtgccgc gtccgtcgcc gccgaaggcg cgtgagaccg gcaccccgcg
     2581 ccctgccagg aggacatccc gatgtcccgt cgtttgcacc gcaccggcgg cgcgctcgcg
     2641 ctgctgtgcc tgccgttcac cctgctcgcg gccccagccg atgccgatct gcccggggcc
     2701 acgctcgccg gtctgatcga atgggctgaa acccgcaatc ccgaactcgc cgtggccggc
     2761 cacgagctcg atgccgccga ggcgatggtc ggttcggccg atgcgctgcc cgacccgagc
     2821 ttcagcatcg agtggcgcga catcgacgcc gatcgcctga cgctcgatcc gagccgcgtc
     2881 ggctcgacga agtacacgtt cgcgcagacc ttcccgctgt ggggcaagcg cgggctgcag
     2941 gccgacatcg cgcgcgccga agccgagcgc gcgcgccgcc agcgcagctc ggtgtcggtc
     3001 gagctgcgcg cccggctcag cgccgcccac gccgaacgct ggcaggtgcg cgagctgctg
     3061 cggctcaacg ccgagcagcg cagcctgctg cgcgatctgg agcgcatcgc caccgcccgc
     3121 tatgccgacg gcctcaccgc ccagtcggat gcgatccggg cgcagaccga acgcacgcag
     3181 ctcgaagccg agcgcgtgcg gctcgaagcc gagcgccgcc ggctcggggc gcagctcaac
     3241 gcgctgctcg ggcgccggcc ggat
//
LOCUS       DAOQIE010000383         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19121_length_3262_cov_7.051138, whole genome shotgun sequence.
ACCESSION   DAOQIE010000383 DAOQIE010000000
VERSION     DAOQIE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19121_length_3262_cov_7.051138"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /gene="smc"
                     /locus_tag="PK144_17105"
     CDS             complement(<1..2104)
                     /gene="smc"
                     /locus_tag="PK144_17105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNF67491.1"
                     /translation="MRLSRIRLAGFKSFVDPVTVHLRSNLVGILGPNGCGKSNVIDAV
                     RWVMGESAASRLRGEAMSDVIFSGSATRKPVGQASVELVFDNAEGRLGGAWASYAEIS
                     VRRLVNRDGTSQYFLNGTRCRRRDVTELFLGTGLGPRSYAIVEQGMISRVIDARPEEL
                     RAFLEEAAGISKYKERRRETETRIRHTRENLDRLADVREELERRLQVLARQARAAEQY
                     RALRAGERELKTGLLALRWQALAAATAALEADGARLETALEAAVAAQRAAEAALGRSR
                     GEQEAAAEALDAVQARHYALGTALARLEQALAASRERRAREADELERLARALAGLDTQ
                     LAADREQLEALAAGIAAGEPALALAQAALAGCHEAQAGAEAALADWQAAHDRIVQAGA
                     EAQRLAEVGRARVEALERRALDAGRRSERLRLELDTLAAAGHAQALSEAEITQRLAAE
                     THAEAELQLAQGDADIDARRAQLAQATRALDALRARVESARGRSAALETLQEAALGRR
                     DDGLQPWLQAQALHAAPRLAEQIAVAPGWETAVEAALGPALEAVLVEALNPPATATPA
                     LLRGALMLVEHAGPASGEAVPGAAGMPDMPGMPGAADASAAAAGVTPLRQCVRGPYAL
                     AGLLGHVHAVGDLDAALAARAHLAAHECLVTADGVLLGRDWLRLARGAADAQHGVLAR
                     ERELRTLREALAADVLELQ"
     gene            complement(2331..>3262)
                     /locus_tag="PK144_17110"
     CDS             complement(2331..>3262)
                     /locus_tag="PK144_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF67492.1"
                     /translation="LESVLDEAQLVAKSDVSVFVHGESGTGKELLAQAIHKASPRATR
                     PFVPVNCGAIPSELLESELFGHVKGAFTGANTAHDGLFVAATGGTLFLDEIGDLPLAM
                     QAKILRALQERQVRPVGSTRNVEVDVRIISATHRDLDQLVADGSFREDLYYRLNVVEL
                     RLPPLRERREDVPLLVEQFLARLAEKAGEASKRFAPEAMEMLISSDWPGNVRQLANVV
                     EHCVALSPSAVIPVALVRRALRDRNTPFPPLEEARDAFEHDYLVRVLRLTRGGVTQAA
                     RLAGRNRTEFYRLLARHGLEPALFKAEAEDSED"
BASE COUNT          403 a         1180 c         1246 g          433 t
ORIGIN      
        1 cctgcagttc gagcacgtca gcggccagcg cctcgcgcag cgtgcgcagc tcgcgctcgc
       61 gcgccagcac gccgtgctgc gcatcggccg ccccgcgcgc aagccgcagc cagtcgcgcc
      121 cgagcagcac gccgtcggcc gtgaccaggc attcgtgcgc ggccagatgc gcgcgggcgg
      181 cgagcgcggc gtcgagatcg ccgaccgcgt ggacgtggcc gagcaggccg gcgagcgcgt
      241 acgggccccg cacgcactgg cgcagcggcg tgacgcccgc agcagcggcg gacgcatcgg
      301 cggcgccggg cattccgggc atgtctggca ttccggcggc tccgggcacc gcttcgccgg
      361 aggccggccc ggcgtgctcg accagcatca gcgcgccgcg cagcagcgcc ggcgtcgccg
      421 tggccggcgg gttcagcgcc tcgaccagca cggcttcgag cgccgggccg agcgcggcct
      481 cgacggcggt ttcccagccc ggagcgacgg caatctgctc ggcgagccgc ggtgcggcat
      541 gcagggcctg cgcctgcagc cagggctgca ggccgtcgtc ccgccgcccg agcgcggctt
      601 cctgcagggt ttcgagcgca gcactgcggc cgcgggcgga ctcgacgcgc gcgcgcagcg
      661 catcgagcgc gcgggtggcc tgcgcaagct gcgcgcggcg ggcgtcgatg tcggcatcgc
      721 cctgcgccag ctgcagttcg gcctcggcat gggtttcggc ggccagccgc tgcgtgattt
      781 cggcctcgct cagtgcctgc gcgtggccgg cggcggcgag cgtgtcgagt tcgaggcgca
      841 ggcgttcgct gcgccggccg gcatcgagtg cgcggcgctc cagcgcctcg acccgcgccc
      901 ggccgacctc ggccagacgc tgcgcctcgg cgccggcctg cacgatgcga tcgtgcgcag
      961 cctgccagtc ggcgagcgcg gcctcggcac cggcctgtgc ctcgtggcag ccggcgagcg
     1021 cggcctgcgc aagcgcaagc gccggttcgc cggcggcgat gccggctgcg agggcttcga
     1081 gctgctcgcg atcggccgcg agctgcgtat cgagcccggc gagcgcgcgc gcgaggcgtt
     1141 cgagttcatc ggcctcgcgc gcgcggcgct cgcgactcgc cgccagcgcc tgttcgaggc
     1201 gcgcgagcgc cgtgccgagc gcgtagtggc gggcctgcac cgcgtcgagc gcctcggcag
     1261 cggcctcctg ttcgccgcga ctgcggccga gcgcggcctc ggcggcgcgc tgcgcggcga
     1321 cggcggcctc cagcgcggtt tccagccgcg cgccgtcggc ctccagcgcg gcggtggccg
     1381 cggccagcgc ctgccagcgc agtgccagca gcccggtctt cagctcgcgc tccccggcgc
     1441 gcagcgcccg gtactgctcg gcggcacgcg cctgccgggc cagcacctgc aggcggcgct
     1501 cgagttcctc gcgcacgtcg gcgaggcggt cgaggttctc gcgcgtgtgg cggatgcggg
     1561 tttcggtctc gcgccggcgt tccttgtact tcgagatgcc ggcagcctct tcgaggaagg
     1621 cacgcagttc ctcgggccgg gcgtcgatga cgcgcgagat catgccctgc tcgacgatcg
     1681 cgtagctgcg cggcccgagg ccggtgccga ggaacagctc ggtcacgtcg cgccgccggc
     1741 agcgcgtgcc gttcaggaag tactggctgg tgccgtcgcg gttgacgagg cggcgcaccg
     1801 agatctcggc gtagctcgcc caggcgccgc cgaggcggcc ttcggcgttg tcgaacacca
     1861 gctcgacgct ggcctggccg acgggcttgc gcgtcgccga accgctgaag atcacgtcgc
     1921 tcatcgcctc gccgcgcagg cgcgaggccg ccgattcgcc catcacccag cgcacggcgt
     1981 cgatgacgtt ggacttgccg cagccgttcg ggccgaggat gccgacgagg ttcgaacgca
     2041 ggtggaccgt gacgggatcg acgaaggact tgaatccggc gaggcgtatc cgggacaggc
     2101 gcatggcggg caaggatacc cgaggcgccg gacgggcggg attgcggttg tgcggatgca
     2161 gcacgcacgg cacggccgga atggccggcg gaagctaagg gtgcagacgg tgatgcaggc
     2221 ggacaggtgc gctgcggatc ccgagcggtg ctgcgtgcgg aggggccccg tcccccgacg
     2281 tgtgcgccgt cgttgcgatc tgcgccggca ggccgctgcc gggatggcgg tcagtcctcg
     2341 ctgtcctcgg cctcggcctt gaacagcgcc ggctccagcc cgtggcgggc gagcagccgg
     2401 tagaactcgg tgcggttgcg cccggccagc cgcgcggcct gcgtgacgcc gccgcgggtc
     2461 aggcgcagca cgcgcaccag atagtcgtgc tcgaaggcat cgcgtgcctc ctccagcggc
     2521 gggaacggcg tgttgcggtc gcgcagcgcg cggcgcacca gcgccaccgg gatcaccgcc
     2581 gacggcgaca gcgccacgca gtgctcgacg acgttggcga gctggcggac gttgccgggc
     2641 cagtcggagg aaatcagcat ctccatcgcc tcgggcgcga agcgcttcga cgcctcgccg
     2701 gccttctcgg ccagccgcgc gaggaactgc tcgaccagca gcggcacatc ctcgcgccgc
     2761 tcgcgcagcg gcggcaggcg cagctcgacg acgttcaggc ggtagtagag gtcctcgcga
     2821 aagctgccgt cggcgacgag ctggtcgagg tcgcggtgcg tggccgagat gatgcgcaca
     2881 tcgacctcga cgttgcgggt cgagccgacc gggcgcacct gacgctcctg cagcgcgcgc
     2941 aggatcttcg cctgcatcgc cagcggcagg tcaccgatct cgtcgaggaa cagcgtgccg
     3001 ccggttgcgg cgacgaacag gccgtcgtgc gcggtgttgg cgccggtgaa cgcacccttc
     3061 acgtggccga aaagctcgga ttcgagcagc tcggacggga tcgcgccgca gttgaccggc
     3121 acgaacggcc gcgtggcgcg cgggctggcc ttgtggatgg cctgcgccag cagttccttg
     3181 ccggtgccgc tctcgccgtg cacgaacacc gagacgtcgg acttggcgac gagctgcgcc
     3241 tcgtcgagca cggattcgag cc
//
LOCUS       DAOQIE010000384         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19122_length_3262_cov_6.782351, whole genome shotgun sequence.
ACCESSION   DAOQIE010000384 DAOQIE010000000
VERSION     DAOQIE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19122_length_3262_cov_6.782351"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK144_17115"
     CDS             <1..475
                     /locus_tag="PK144_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF67493.1"
                     /translation="LAEARRVLDVNLLAPFLLMQAVIPHLIAAGGGAIVNVASDQAFV
                     GKKGSAIYGASKAALAQLARSAALDWAAHGIRVNAIAPGSTDTPMLRQVFADLRRRYP
                     EAFPVEGAEAYVGAIPLGRLAEPREIAWTIAFLASDAASFITGTVLAVDGGFTAA"
     gene            517..1248
                     /locus_tag="PK144_17120"
     CDS             517..1248
                     /locus_tag="PK144_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF67494.1"
                     /translation="MTKNADTPVVLVTGASRGIGLAIVQAFAGAGWRTAGCARTPGPA
                     LAAAADLVQGCDVSDGAAVHALVEAVLGRFGRLDALVCNAGLAGSNPVGSEDDDARWR
                     AILATNLDGTWHACRAALPRLPDGHGRIVNIASVLALRGVADQPAYCAAKHGVLGLTR
                     SLALQAAARGITVNAICPGWTRTAMAEARYAELGIDAAQAAAGVPLGRIVEPAEVAAL
                     ALWLCSPAAGAITGQALTIDGGTLA"
     gene            1497..1904
                     /locus_tag="PK144_17125"
     CDS             1497..1904
                     /locus_tag="PK144_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67495.1"
                     /translation="MSWFLALLVAALSAWMIRVFVRLPPRVERTRTPAPAAGRAQPWQ
                     AVRIVPGAGACAAAHAQAARLYPPGHAPLMPLADGGCEHCHCQYETIQDRRRGERRSG
                     MDRRTGLRYEPDHSDRRASRERRRTFGWSGLPT"
     gene            complement(1999..3093)
                     /locus_tag="PK144_17130"
     CDS             complement(1999..3093)
                     /locus_tag="PK144_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF67496.1"
                     /translation="MPAQNIVPNTVATSAAHIEPCHGYAFDGDLVRLYAQIAVDAVPV
                     ANAEWAVQLWACATPFAGGELHGTKIAEVRAAVAAEAPAWLDATTTALPPAGAGEHAM
                     VLVLASGDDGHFAAIHDYANYPHAERFVLPRLGGTVGYRIDGGSVTLEVGSVANPRPA
                     DNLSGSLALEFWALAAPYAGADAFAGSKLAGVDLGTLAGGCALDGLTFTLSCAGQPDG
                     VWYPVLMLREWTAAGWVTRDYTAFAPSGTAAPLAAAPVAQAPAVETAPLAEAPVIEAP
                     AAAPAVVEPAPAVKPAKAKARKAKAAASGPAVSVNTSTALALAEVKGISKTVAAAIVA
                     ARPYASLDDLLRAKGVGAKLLEKLRAQLTL"
BASE COUNT          462 a         1197 c         1150 g          453 t
ORIGIN      
        1 gctcgccgaa gcgcggcgcg tgctcgacgt gaacctgctc gcccccttcc tgctgatgca
       61 ggcggtgatt ccgcacctga tcgcggccgg aggcggcgcg atcgtcaacg tcgccagcga
      121 tcaggccttc gtcggcaaga agggctcggc gatctacggc gccagcaagg ccgcgctcgc
      181 acagcttgcg cgcagcgccg cgctcgactg ggctgcgcac ggcatccgcg tcaacgccat
      241 cgcccccggc agcaccgaca cgccgatgct gcgtcaggtg ttcgccgacc tgcgccggcg
      301 ctacccggag gccttccccg tcgagggcgc cgaggcctac gtcggcgcga tcccgctcgg
      361 gcggctggcc gagccgcgcg agatcgcgtg gacgatcgcg tttctcgcct cggacgccgc
      421 ttcgttcatc accggcaccg tgctggcggt cgacggcggc ttcacggccg cctgaaccgg
      481 atcagcggct gattcgatga caagatgaac catacgatga caaaaaatgc agacaccccg
      541 gtcgtactgg tcaccggcgc ctcgcgcggc atcgggctgg cgatcgtgca ggcctttgcc
      601 ggcgccggct ggcgcacggc cggctgcgcg cgcacgccag gcccggcact ggctgcagcc
      661 gccgatctgg tgcagggctg cgacgtgagc gacggcgcgg ccgtgcacgc gctggtcgag
      721 gcggtgctcg ggcgcttcgg ccggctcgat gcgctggtct gcaatgccgg cctcgccggc
      781 agcaatccgg tcggctccga ggacgacgat gcgcgctggc gcgccatcct cgcgaccaac
      841 ctcgacggca cctggcacgc ctgccgcgcc gcgctgccgc ggctgcccga tggacacggc
      901 cgcatcgtca acatcgcctc ggtgctggca ctgcgcggcg tggcggacca gccggcctac
      961 tgcgcggcca agcacggcgt gctcgggctg acgcgctcgc tggcgctgca ggcggcggcg
     1021 cgcggcatca ccgtcaacgc catctgtccg ggctggacgc gcacggcgat ggccgaggcc
     1081 cgctacgccg aactcggcat cgacgccgcg caggccgccg ccggcgtgcc gctcgggcgc
     1141 atcgtcgagc ccgcggaggt cgcggcgctg gcgctctggc tgtgctcgcc ggcggccggc
     1201 gccatcaccg gccaggcgct gaccatcgac ggcggcacgc tcgcctgaag ccgtcgacgg
     1261 cgctcaagtc cggacggatc cggacgatca tgtgactaaa ctttacgaaa aatcctccgg
     1321 cggcagagcg gaggtcgccg gcgcgctcca gcgcgatatc cggtgcgccg tggatcggtc
     1381 ccggagcgga cctgccagga ggccggtgcc gaccggacag cccggccggt gatcgtccag
     1441 acaagacgtt ccggcggact cgcgccggcg cgcgggttcc gacgatgtcc ctgtccatgt
     1501 cctggtttct cgccctgctg gtggctgccc tgtcggcctg gatgatccgg gtgttcgtgc
     1561 gtctgccgcc gcgcgtcgaa cgcacgcgca cgcccgcacc cgcggccggt cgcgcgcagc
     1621 cctggcaggc ggtccgcatc gtgcccggcg ccggcgcctg tgcggctgcc cacgcgcagg
     1681 ccgcccggct gtatccgccc ggccacgcac cgctgatgcc gctcgccgat ggcggctgcg
     1741 agcactgtca ttgccagtac gaaaccatcc aggaccggcg ccgcggcgaa cggcgcagcg
     1801 gcatggaccg gcgcaccggc ctgcgctacg agccggatca ctcggaccgg cgcgccagcc
     1861 gcgagcgccg gcgcacattc ggctggagcg gcctccccac ctgatcccgt gacctgatct
     1921 gatcccggga cgaatctccc gccgccgcgc cgccgcccgt gcagcgggcg accaccggcc
     1981 gcatgagacg cggccggctt acagcgtcag ctgcgcacgc agcttttcga gcagcttggc
     2041 gccgacgccc ttggcccgca gcagatcgtc gaggctcgca tacgggcggg cggcgacgat
     2101 ggcggcagcg acggtcttcg aaatgccctt gacctcggcc agcgccagcg cagtcgaggt
     2161 gttcaccgac accgccggtc ccgacgccgc ggcctttgcc ttcctggcct tggccttggc
     2221 cggcttcacg gccggtgcag gctcgacgac cgcaggcgcg gcggccggcg cctcgatgac
     2281 cggcgcttcg gcaagcggag cggtttcgac ggccggggct tgcgccaccg gggcggcggc
     2341 cagcggcgcg gcagtgcccg agggcgcgaa ggcggtgtag tcacgcgtga cccagccggc
     2401 ggccgtccac tcgcgcagca tcagcaccgg ataccagacg ccgtccggct ggccggcaca
     2461 gctcagcgtg aaggtcaggc cgtcgagcgc gcagccaccg gccagcgtgc cgagatcgac
     2521 gccggcgagc ttgctgccgg cgaacgcatc ggcaccggca tacggcgcgg cgagcgccca
     2581 gaactcaagc gcgagcgaac cgctgaggtt gtcggccgga cgcgggttgg cgaccgatcc
     2641 gacttcgagc gtgacgctgc cgccgtcgat ccggtagccg accgtgccgc cgaggcgcgg
     2701 cagcacgaag cgttcggcgt gcggatagtt cgcatagtcg tggatcgccg cgaagtggcc
     2761 gtcgtcgccg ctggcgagca cgagcaccat cgcatgctcg ccggccccgg ccggcggcag
     2821 cgccgtcgtg gtcgcatcga gccaggccgg cgcctcggcc gccaccgcgg cgcgcacctc
     2881 ggcgatcttg gtcccgtgca gttcaccgcc ggcaaacggc gtcgcgcagg cccagagctg
     2941 cacggcccat tcggcattgg ccaccggcac ggcatcgacg gcgatctgcg cgtacagacg
     3001 caccagatca ccatcgaagg cgtaaccgtg gcagggttcg atatgggcgg cagacgtggc
     3061 gacggtgttc ggaacgatat tctgagctgg catttcggca atgttcccaa tacccggaaa
     3121 gggcggggga ttatacagat tctgcttccg atgaaaatgc acgcgctccg ggatgccggc
     3181 tattggctgg caattcccgg acacggcacg gtaatttttc ccctttacca tcagcaggct
     3241 ggacaccggc acaccggcca tg
//
LOCUS       DAOQIE010000385         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19128_length_3261_cov_5.484404, whole genome shotgun sequence.
ACCESSION   DAOQIE010000385 DAOQIE010000000
VERSION     DAOQIE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19128_length_3261_cov_5.484404"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK144_17135"
     CDS             <1..708
                     /locus_tag="PK144_17135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF67497.1"
                     /translation="LALLTWRYVRDRPEDAGLPPLHALPPAAARPHWSKELAAVLRER
                     RLWPVVLVQFGMIGGFYAFVGLWAVPLLERAHGLARPAAAAHVTTALAVFALGSLTLG
                     GLSDRLGRRKPVLVGAVGLYLLACLALAAAPWTPGGGGHALFAALGLGAAGLTITFPL
                     GRELGPPALAGMAISVVNSGAFLGTALMQPLFGALVDRSGGFAAGLWLLAAGAVLALA
                     AALACTETRCRNLAAAR"
     gene            complement(705..1106)
                     /gene="arfB"
                     /locus_tag="PK144_17140"
     CDS             complement(705..1106)
                     /gene="arfB"
                     /locus_tag="PK144_17140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNF67498.1"
                     /translation="MHIPDLPEAELDFRPIRAQGPGGQNVNKVETAVQLRFDIGASSL
                     PEAVKARLLRLRDARIHAGGIIVIKAQRHRSQADNRADALARLQALIRSVWTAPKPRR
                     PTQPSRAARERRLDAKQHRGRIKALRAPPGD"
     gene            complement(1122..1637)
                     /locus_tag="PK144_17145"
     CDS             complement(1122..1637)
                     /locus_tag="PK144_17145"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNF67499.1"
                     /translation="MPSPRDSLPAVTPAFDPETPAGIGGWLLLMLLGLVLTPLRVAWS
                     TYGMLQPVLMPGVWEQLTEPGQPVYHPLWAPLIVAETAGNGLTLLLALLALWLFLRRS
                     RRAPAVIIGLFVWSLLFAITDQAATGLIPMIPPPTTETLVDALVAPAVAVAIWVPYLL
                     RSKRVRATFVL"
     gene            complement(1670..2761)
                     /locus_tag="PK144_17150"
     CDS             complement(1670..2761)
                     /locus_tag="PK144_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNF67500.1"
                     /translation="MNALLPRLRTLAAALATLALLAHAATGTAEPLRRYNVDPASVSV
                     SGISSGGYMAVQFAVAYSGIVHGVGVVAGGPYGCANASITTALDVCMAGKRPIRVADL
                     VSLTKRRARSGAIDPVAGLAAQRVWIFTGSIDSTVVPKVGDALEQYYRAFVPAASITR
                     VSSVRAEHTMPTTDFGNDCTIKGDPYISDCDFDGAGELLQWIHGPLQPPAATAPGGRL
                     TPFEQNEFSSFPAAHSLGSTGWVYVPQACEDGARRCRLHVAFHGCRQFADFLYVDANN
                     QPARYGTTFAEHAGYNRWADANDFIVLYPQTRRGPELPGDLFGVTSANPKGCWDWWAY
                     DSTQYAEKNGPQMLAVRRMIARISGEQPH"
     gene            complement(2843..>3261)
                     /locus_tag="PK144_17155"
     CDS             complement(2843..>3261)
                     /locus_tag="PK144_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67501.1"
                     /translation="QTLATLADTCAFWQREVAAGRNGSAMRDDACQRLQRFAAEQRLA
                     LPGNTVAAAGLPAAAVSRRIEVSAAPAVAVHDCAAWGPGSIAYRDCRQRERARLDAWC
                     RQLNRDADRLGGEARQTALDWREAVCSAAERYTVMR"
BASE COUNT          433 a         1134 c         1226 g          468 t
ORIGIN      
        1 ctggcgctgc tgacctggcg ctacgtgcgc gaccgccccg aggacgccgg tctgccgccg
       61 ctgcacgcgc tgccgccggc cgccgcccgg ccgcactggt cgaaggaact cgccgccgtg
      121 ctgcgcgagc gccggctctg gccggtggtc ttggtgcagt tcggcatgat cggcggcttc
      181 tacgccttcg tcggcctctg ggccgtgccc ttgctcgaac gcgcccacgg ccttgcgcga
      241 ccggccgcgg ccgcccatgt gacgacggcg ctcgcggtgt tcgcgctcgg ttcgctgacg
      301 ctcggcggac tgtcggaccg gctcgggcgg cgcaagccgg tgctggtcgg agcggtcggg
      361 ctgtatctgc tcgcgtgtct tgcgctcgcg gcagcgccgt ggaccccggg cggcggcggg
      421 catgcgctgt tcgcagccct cggcctcggc gccgccgggc tgacgatcac cttcccgctc
      481 gggcgcgaac tcgggccgcc ggcgctcgcc ggcatggcga tcagcgtcgt caacagcggc
      541 gcattcctcg gcacggcgct gatgcagccc ttgttcggcg cgctggtcga tcgcagcggc
      601 ggtttcgcgg ccggactttg gctgctcgcc gccggtgccg tgctggccct ggcggcggcg
      661 ctggcctgta ccgaaacgcg ctgtcgcaac ctcgccgctg cccgctagtc gccgggaggc
      721 gcgcgaagcg ccttgatccg gccgcgatgc tgcttggcat cgagccggcg ctcacgggcg
      781 gcgcggctcg gctgcgtcgg ccggcgcggt ttcggtgccg tccagacgga gcggatcagc
      841 gcctgcaggc gcgcgagcgc atcggcacgg ttgtcggcct gcgaacggtg gcgctgggcc
      901 ttgatgacga tgatgccgcc ggcgtggatg cgcgcatcgc gcaggcgcag cagccgcgcc
      961 ttgaccgcct cgggcagcga cgaggcgccg atgtcgaagc gcagctgcac ggcggtctcg
     1021 accttgttga cgttctggcc gcccggcccc tgcgcgcgga tcggccggaa gtcgagttcg
     1081 gcctcgggca ggtcagggat gtgcatgccg gacaccggac ttcagagcac gaaggtcgcg
     1141 cgcacgcgct tcgagcgcag caggtacggc acccagatcg cgaccgcgac cgccggtgcg
     1201 accagcgcat cgaccagcgt ttccgtggtg ggcggcggaa tcatcgggat cagcccggtg
     1261 gccgcctggt cggtgatcgc gaacagcagc gaccagacga acaggccgat gatcacggcc
     1321 ggtgcccgcc gcgagcggcg caggaacagc cacagcgcca gcagcgcgag cagcagcgtc
     1381 aggccgttgc cggcggtttc ggcgacgatc agcggtgccc acagcgggtg atagaccggt
     1441 tggccgggct cggtcagctg ctcccagacg ccgggcatca gcaccggctg cagcatcccg
     1501 tacgtcgacc aggccacgcg cagcggcgtc agcaccagcc cgagcagcat cagcagcagc
     1561 cagccgccga tgccggccgg ggtttcgggg tcgaaggccg gcgtgacggc cggcagggaa
     1621 tcgcgggggc tcggcatcgg ggtgtccgga ggtctgggca gggcgggctt cagtgtggct
     1681 gctcgccgct gatgcgcgcg atcatccggc gcacggcgag catctgcgga ccgttcttct
     1741 cggcgtactg cgtgctgtcg taggcccacc agtcccagca gcccttcgga ttggccgacg
     1801 tgaccccgaa caggtcgccc ggcagctccg ggccgcgccg ggtctgcgga tacagcacga
     1861 tgaagtcatt ggcatcggcc cagcggttgt agccggcgtg ctcggcgaag gtcgtgccgt
     1921 agcgggccgg ctgattattc gcatccacgt acaggaagtc cgcgaactgg cggcagccgt
     1981 ggaaggcgac gtgcagccgg cagcggcgcg ccccgtcctc acaggcctgc ggcacgtaga
     2041 cccagccggt gctgccgagg ctgtgtgcgg ccgggaagct gctgaactcg ttctgttcaa
     2101 agggcgtcag gcggccgccc ggtgcggtgg ctgccggcgg ctgcagcggc ccgtggatcc
     2161 actgcagcag ctcgccggcg ccgtcgaaat cgcagtcgct gatgtacggg tcgcccttga
     2221 tcgtgcagtc gttgccgaag tcggtggtcg gcatcgtgtg ctcggcgcgc acgctgctga
     2281 cgcgcgtgat cgaggcggcc ggcacgaagg cccggtaata ctgttccagc gcatcgccaa
     2341 ccttcggcac gacggtcgag tcgatgctgc cggtgaagat ccacacgcgc tgcgcggcca
     2401 ggcccgcgac cgggtcgatc gcgccgctgc gcgcccggcg cttggtcagc gacaccagat
     2461 cggcgacgcg gatcggacgc ttgccggcca tgcacacgtc gagcgcggtc gtgatgctgg
     2521 cgttggcgca accatacggg ccgccggcga cgacgccgac cccgtgcacg atccccgaat
     2581 aggcgaccgc gaactgcacg gccatgtagc cgccggagga aatccccgat accgacacac
     2641 tggccgggtc gacgttgtag cggcgcagcg gttcggccgt gccggtggcg gcgtgcgcga
     2701 gcagggccag cgtggcgagt gcggcagcga gcgtgcgcag gcggggcagg agagcattca
     2761 tgccggggtc ctcattcgac ggcgacaggg gcgggcatcg ccgcaccggg gggcgcgcgg
     2821 cgcacgtcag gggatttcgg gttcagcgca tgaccgtata ccgctctgcg gccgagcaga
     2881 cggcttcgcg ccagtcgagt gccgtctgcc gggcctcgcc gccgaggcga tcggcatcgc
     2941 ggttgagctg ccggcaccag gcatcgagcc gggcacgctc ccgctggcgg cagtcgcgat
     3001 acgcgatcga gccgggaccc caggccgcgc agtcatggac ggcaacggcc ggggcggcgc
     3061 tcacctcgat ccggcgtgac acggcagccg ccggcagccc tgcggccgcg acggtattgc
     3121 cgggcagggc cagtcgctgt tcggcggcga agcgctgcag ccgctggcag gcatcgtccc
     3181 gcatcgccga accgttgcgg ccggcagcga cctcgcgctg ccagaacgca caggtatcgg
     3241 cgagcgtggc gagcgtctgc c
//
LOCUS       DAOQIE010000386         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19753_length_3197_cov_6.121260, whole genome shotgun sequence.
ACCESSION   DAOQIE010000386 DAOQIE010000000
VERSION     DAOQIE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19753_length_3197_cov_6.121260"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2959)
                     /locus_tag="PK144_17160"
     CDS             complement(<1..2959)
                     /locus_tag="PK144_17160"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF67502.1"
                     /translation="MHSFWPLIPIAALLIAGGGGLYAGSELRHTQERLAAAGQERVEV
                     ATATLRATMRGAVADITYLAALPDLHGALRDNDPDGLAAVAAEYVAYMLSHRDFDQIR
                     WIDASGRERVRVDYRDGQPYVVPPEALQSKADRPYFIETMQLPAGAIHQSPLDLNVEH
                     GQIERPFKPVLRFAMPLFDAEGRRHGIVVLNYLGRVLLDAVRQTSRQQAGELMLLNGD
                     GYWLLAPEPADEWGFQLDPARTLARRAPALWSALQAQVSGSLKQDDGLWLWLGLPAHL
                     SITEGAPDEFWHMALRIPQATLVAARQHIVTQVGSVVAVLLAATALVGIGFQRTRRQL
                     AHLHERLAERAEAAEAGAHAKAVFLANMSHEIRTPMNAILGLAYLLERMPLPAEAGEL
                     VRRIRRSGRTLQTILNDILDFSKIEAGRLELEHAAFRLGDVLDDLSSLMAAACADKEL
                     ELVLSAPPGVDSLTGDALRLAQVLINLTGNAIKFTEHGQVSVEVRVLEADAQRVRLEF
                     AVRDTGIGIEPARQQELFAAFVQADTSTTRRFGGTGLGLAISRRLVALMGGALEVESA
                     PGLGSTFRFTLGFERGPQLPLSVPDLAGLDVLIVDDNETSRAALCGTALQLGWKPVAL
                     DSGTAAVEHVRSHFPRDPDRSRTVIVLDWQMPGMDGLATARAIRSLTGDRREPILLMA
                     TTFSCAKLQALDDSRMIDAILSKPVTPSALHDAVVRTLCRQGAAASPAPVTSQPPRLP
                     GLRVLVVDDSDINRDVARRILAGEGARVSLASDGRQAVEWLKAHVGAVDLVLMDVQMP
                     VMDGHEATCAIRRTAGLSRLPVIALTAGAFRDQQDAALAAGMNDFIAKPFDADAAVTL
                     IARVARSAGWQPAAATGASAVSRAPPLPAEPAPVLDLPQALALWNDAAVLRRYLQRFA
                     AEYGDCAARLGAAGAAEAAAGVHRLKGTADNLKKIGGLVVNNERQINGIINPLLASII
                     FDRDYTEANRKEVQ"
BASE COUNT          469 a         1067 c         1177 g          484 t
ORIGIN      
        1 gctggacctc cttgcggttg gcctcggtgt agtcgcggtc gaagatgatg gaggcgagca
       61 gcgggttgat gatcccgttg atctggcgct cgttgttgac gaccagtccg ccgatcttct
      121 tgaggttgtc ggccgtgccc ttgagccggt gcacgccggc tgccgcctcg gccgctccgg
      181 cggcaccgag ccgcgcggcg cagtcgccgt actcggcggc gaagcgctgc agatagcggc
      241 gcagcacggc cgcatcgttc cagagcgcca gggcctgtgg cagatcgagc acgggcgcgg
      301 gctcggccgg cagcggcggt gcgcggctga cggcggaggc tccagtggcg gcagccggct
      361 gccagcctgc gctgcgcgcg acgcgggcga tcagggtcac ggcggcatcg gcatcgaagg
      421 gcttggcgat gaagtcgttc atgccggccg cgagggcggc gtcctgctgg tcgcggaagg
      481 cgccggcggt cagcgcgatc accggcagcc gggacaaacc cgccgtgcgc cggatcgcgc
      541 acgtggcttc gtggccgtcc atgaccggca tctgcacatc catcagcacc agatcgacgg
      601 caccgacgtg cgccttcagc cattcgacgg cctggcggcc gtcgctggcg agcgagaccc
      661 gggcgccttc gccggcgagg atgcgccggg cgacatcgcg gttgatgtcg ctgtcatcga
      721 cgacgagcac gcgcaggccc ggcagccgcg gtggctggct ggtcaccggg gcggggctgg
      781 cggcggcgcc ctggcggcag agcgtgcgca cgacggcatc gtgcagcgcc gaaggcgtca
      841 ccggcttgct caggatcgca tcgatcatcc ggctgtcgtc cagggcctgc agcttcgcgc
      901 aggagaaggt cgtggccatc agcaggatcg gttcacgccg gtcgccggtg aggctgcgga
      961 tggcgcgggc cgtcgcgagt ccgtccatgc ccggcatctg ccagtcgagc acgatgacgg
     1021 tgcgcgaccg gtccggatcg cgcgggaagt ggctgcggac gtgttcgacg gccgccgtgc
     1081 ccgaatcgag cgcgaccggc ttccagccga gctgcagtgc cgtgccgcag agcgccgcgc
     1141 gcgaggtttc gttgtcatcg acgatcagca cgtcgagccc ggccagatcg ggcacggaca
     1201 gcggcagttg cgggccgcgt tcaaagccga gcgtgaagcg gaaggtgctg cccaggcccg
     1261 gggcggattc cacctccagt gcaccaccca tcagcgccac cagccggcgg ctgatcgcga
     1321 gtccgagtcc ggtgccgccg aagcggcgcg tcgtcgaggt gtcggcctgc acgaaggcgg
     1381 cgaacagctc ctgctggcgg gcgggctcga tgccgatgcc ggtgtcgcgc accgcaaact
     1441 ccagccgcac gcgctgcgca tcggcttcga gcacgcgcac ctcgacgctg acctgaccgt
     1501 gctcggtgaa cttgatcgcg ttgccggtca ggttgatcag cacctgagcg aggcgcagcg
     1561 cgtcgccggt caggctgtcg acgccgggcg gcgctgacag cacgagttcg agctccttgt
     1621 cggcgcaggc ggccgccatc agcgaggaca ggtcatcgag cacgtcaccg aggcggaagg
     1681 cggcgtgttc gagttcgagc cggccggcct cgatcttcga gaagtcgagg atgtcgttga
     1741 ggatggtctg cagcgtgcgg cccgagcggc ggatgcggcg cacgagttcc ccggcctcgg
     1801 ccggcagcgg catgcgttcg agcaggtagg cgaggccgag gatcgcgttc atcggcgtgc
     1861 ggatctcgtg gctcatgttc gcgaggaaca ccgccttggc gtgcgcaccg gcctcggccg
     1921 cctcggcgcg ctcggcgagc cgctcatgca ggtgagcgag ctggcgccgg gtgcgctgga
     1981 agccgatgcc gaccagcgcc gtggcggcca gcagtacggc gaccacgctg ccgacctgcg
     2041 tgacgatgtg ctggcgggcg gccaccagcg tcgcctgcgg aatgcgcagc gccatgtgcc
     2101 agaactcgtc aggcgcgcct tcggtgatgc tcaggtgcgc cggcaggccg agccagagcc
     2161 agaggccatc gtcctgcttc aggctgccgg agacctgcgc ctgcagggcg gaccagagcg
     2221 ccggtgcgcg ccgggccagc gtgcgcgccg gatcgagctg gaagccccat tcgtcggcag
     2281 gctcgggtgc gagcagccag tagccgtcgc cattgagcag catcagctct ccggcctgct
     2341 ggcgactggt ctggcgtacg gcatcgagca gcacgcgacc gaggtagttg agcacgacga
     2401 tgccgtggcg ccggccctcg gcatcgaaca gcggcatcgc aaagcgcagc accggcttga
     2461 acggccgttc gatctgcccg tgctcgacgt tcaggtcgag cggcgactga tggatcgcgc
     2521 cggccggcag ttgcatcgtc tcgatgaaat acgggcggtc agccttggac tgcagggctt
     2581 cgggcggtac cacgtatggc tggccgtcgc ggtaatcgac gcgcacgcgt tcgcggccgc
     2641 tcgcgtcgat ccagcggatc tggtcgaaat cccgatgcga cagcatgtac gcgacgtatt
     2701 cagcggccac ggctgccagt ccgtcggggt cgttgtcacg caacgcgccg tgcaggtccg
     2761 gcagcgcggc caggtaggtg atgtcggcaa cggcaccgcg catcgtcgcg cgcagcgtcg
     2821 ccgtggccac ctcgacgcgc tcctgaccgg ccgccgcgag gcgttcctgc gtgtggcgca
     2881 gttcggagcc ggcatacagc ccgccgccgc cggcgatcag cagcgccgca atcgggatga
     2941 gcggccagaa agagtgcatg tcagcaagcc gtcgaccggc ggttcctctg caggctttcg
     3001 gtttcggggg caaccggggt tgcctgcctg tcgatcggca aggcaagcca gtccttcctg
     3061 gctggatatc gaccgccacc cctgcaactg aagatgccgg ggaaagcttc cggaccggtt
     3121 ccccggacct tcacgaccct gtcggcgaca gcaatgcctg cgcgtgccag cattgctgtc
     3181 gcctgtctgt acattaa
//
LOCUS       DAOQIE010000387         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19784_length_3194_cov_6.591271, whole genome shotgun sequence.
ACCESSION   DAOQIE010000387 DAOQIE010000000
VERSION     DAOQIE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19784_length_3194_cov_6.591271"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK144_17165"
     CDS             <1..865
                     /locus_tag="PK144_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNF67503.1"
                     /translation="IGGAISQDGRHVAVANYEPGGIRIFDAATLAPLADVPAGYGENG
                     ARAKVVGIADVPGGGFIYSLFEAGEIRLTTLDADGRAQTRRWSAGLQPYDGLISPDGR
                     WYVAGLFGEPGLAVLDLWHPEAGVRKVLQQYGRDDPKLPVYKMPHLRGWAIAGRLAFL
                     PAIGHHELVVVDTDTWSERARIPVRGQPVFAMARPDGRQVWVNFAHPDNGALQVVDVE
                     TLAVIDTLEPGRAVLHLEFTPRGEQVWVSARDSGEVLVYDTQRRTVLARLPADSPSGI
                     FMTARSARIGM"
     gene            881..1387
                     /locus_tag="PK144_17170"
     CDS             881..1387
                     /locus_tag="PK144_17170"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNF67504.1"
                     /translation="MPAAFPAAPSASFVLDALDWRLLDEFQRDFPLDAQPWARIGERL
                     GLGADAVLARLTRLQALGCIARVGPVFAPKRAGASLLAALAVAPQRLAEVAAIVSAQP
                     EVNHNYEREPAGLDFNLWFVLTAADAAALDAAIVRIESATGLTVMRLPLEREYHIDLG
                     FRLHGGRP"
     gene            1384..1914
                     /locus_tag="PK144_17175"
     CDS             1384..1914
                     /locus_tag="PK144_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HNF67505.1"
                     /translation="MNPPRLPEASRATPAGAWRQALIAAVQDGLPLVPEPFAALAATL
                     GCDEAEVREGLAALLADGTVRRLGVVVRHRELGFAANAMVVWDVPDARVDALGAQLAA
                     EPAVTLCYRRPRVPPHWPYNLFCMIHGHDRAAVLAELAAIVGRHGLGELPHRPLFSRQ
                     RFKQRGARYLDRGAPS"
     gene            1911..2384
                     /locus_tag="PK144_17180"
     CDS             1911..2384
                     /locus_tag="PK144_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNF67506.1"
                     /translation="MSRPAATRPALDAADRALINRLQRGLPLVDAPYAAVGAELGLSE
                     AEVLQRLQSLLDRRVLTRFGPLFHAERLGGALLLAALAVPQADFERIAALVNAFPEVA
                     HNYEREHALNMWFVLATATPAALEAAILGIEAATGLPVHRFPKLEEFYVGLYLPA"
     gene            2413..2697
                     /locus_tag="PK144_17185"
     CDS             2413..2697
                     /locus_tag="PK144_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNF67507.1"
                     /translation="MQAIPYTDARNQLARLLDRVTREHQPVLITRRKGEPAVLIALAD
                     FEHWRHEHYLLRRPDEARRLLDTLDEIRDGRHGEPQDPPQDGIDRSGTQP"
     gene            2694..3194
                     /locus_tag="PK144_17190"
     CDS             2694..3194
                     /locus_tag="PK144_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF67508.1"
                     /translation="MNPQSPEAADAALERALILATQAGLPLTPRPFATLAEQLGSDAD
                     TVRAGFAAMQARGAIRRIGALPDHYALGYVANGMTVWDIDDAQLHDCGRRLGALGFVS
                     HCYHRPRSLPAWPYNLFAMVHAHDRAEVEARRAEMRALLGSACRADAVLYSTRILKKT
                     GLRIGA"
BASE COUNT          426 a         1237 c         1092 g          439 t
ORIGIN      
        1 gatcggcggc gccatctcgc aggacggccg ccatgtcgcg gtcgccaact acgaacccgg
       61 cggcatccgc atcttcgatg ccgcgacgct cgcgccgctc gccgacgtgc cggccggata
      121 cggcgaaaac ggcgcgcgcg ccaaggtggt cggcatcgcc gacgtgccgg gcggcggctt
      181 catctattcg ctgttcgagg ccggcgagat tcgcctgacc acgctcgatg ccgacggccg
      241 cgcgcagacg cggcgctgga gcgccggcct gcagccctac gacggcctga tctcgcccga
      301 cggccgctgg tacgtcgccg ggctgttcgg cgagccgggg ctggcggtgc tcgatctctg
      361 gcacccggag gccggcgtgc gcaaggtgct gcagcagtac gggcgcgatg acccgaagct
      421 gccggtctac aagatgccgc acctgcgcgg ctgggcgatc gccgggcggc tggccttcct
      481 gccggcgatc ggtcatcacg agctggtcgt cgtcgatacc gatacctgga gcgagcgagc
      541 gcgcattccg gtgcgcggcc agccggtgtt cgcgatggcg cggcccgatg gccggcaggt
      601 gtgggtgaac ttcgcgcatc cggacaacgg cgcgctgcag gtcgtcgatg tcgagacgct
      661 cgccgtcatc gacacgctcg aaccgggcag ggccgtgctg catctggagt tcacgccgcg
      721 cggcgagcag gtctgggtgt cggcgcgcga cagcggcgag gtgctggtct acgacacgca
      781 gcgccgcacc gtgctcgcgc gcctgccggc cgacagcccg agcggcatct tcatgaccgc
      841 gcgcagcgcg cgcatcggca tgtgaacgga gaccgccgcg atgcctgccg ccttccctgc
      901 cgccccttct gccagcttcg tgctcgatgc gctcgactgg cgcctgctcg atgaattcca
      961 gcgcgacttc ccgctcgacg cgcaaccctg ggcgcgcatc ggcgaacggc tcgggctcgg
     1021 cgccgatgcc gtgctcgcgc ggctgacgcg gctgcaggcg ctcggctgca tcgcccgcgt
     1081 cgggccggtg ttcgcgccga agcgcgccgg cgccagcctg ctcgccgccc tcgccgtggc
     1141 gccgcaacgc ctcgccgagg tcgccgccat cgtcagcgcc cagcccgagg tcaaccacaa
     1201 ctacgaacgc gagccggccg gcctcgactt caacctgtgg ttcgtgctga ccgcggccga
     1261 tgccgcagca ctcgatgccg ccatcgtccg catcgagtcc gcgaccgggc tgacggtgat
     1321 gcgcctgccg ctggagcgcg aataccacat cgacctcggc ttccggctgc acggaggccg
     1381 gccatgaacc cgccacggct cccggaggcc tcgcgcgcca ccccggccgg agcgtggcgt
     1441 caggcgctga tcgccgccgt gcaggacggc ctgccgctgg tgccggaacc gttcgcagcc
     1501 ctcgccgcca cgctcggctg cgacgaggcc gaggtgcgcg agggcctcgc cgcgctgctc
     1561 gccgacggca ccgtgcgccg gctcggcgtg gtcgtgcgcc accgcgaact cggcttcgcg
     1621 gccaacgcca tggtggtctg ggacgtgccg gacgcgcgcg tcgatgcact cggcgcgcaa
     1681 ctcgccgccg agccggcggt gacgctctgc taccgccgcc cgcgcgtgcc gccgcactgg
     1741 ccgtacaacc tgttctgcat gatccacggg cacgaccgcg ccgccgtgct cgcggaactg
     1801 gcggcgatcg tcgggcgcca cggcctcggc gagctgccgc accggccgct gttcagccgc
     1861 cagcgcttca agcagcgcgg cgcgcgctac ctcgaccgcg gagccccgtc atgagccggc
     1921 ccgccgccac gcggcctgcg ctcgatgccg ccgatcgcgc gctgatcaac cgcctgcagc
     1981 gcggcctgcc gctggtcgat gcgccgtatg cggcggtcgg ggccgaactc ggcctgagcg
     2041 aggccgaggt actgcagcgg ctgcaaagcc tgcttgatcg gcgcgtgctg acgcgcttcg
     2101 ggccgctgtt tcatgccgag cggctcggcg gcgcgctgct gcttgcagcc ctcgcggtgc
     2161 cgcaggccga cttcgagcgc atcgccgccc tcgtcaacgc cttccccgag gtcgcgcaca
     2221 actacgaacg cgagcatgcg ctgaacatgt ggttcgtgct cgccaccgca acgccggccg
     2281 ccctcgaagc cgcgatcctc ggcatcgagg ccgccaccgg cctgccggtc caccgttttc
     2341 cgaaactcga agagttctac gtcggcctgt acctgccggc gtgaaccgca gccacgcgct
     2401 gggagccccc cgatgcaagc gattccgtat accgatgccc gcaaccagct cgcccgcctg
     2461 ctcgatcgcg tcacgcgcga gcaccagccg gtactgatca cccggcgcaa gggcgaaccg
     2521 gcggtgctga tcgcgctcgc cgacttcgag cactggcgcc acgagcacta cctgctgcgc
     2581 cgccccgacg aggcccgccg cctgctcgac accctcgacg agatccgcga cggccggcac
     2641 ggcgagccgc aggacccgcc gcaggacggc atcgaccgct ccggtacgca gccgtgaatc
     2701 cgcagtcgcc cgaggctgcc gacgccgccc tcgaacgcgc gctgatcctc gccacccagg
     2761 ccgggctgcc gctgacgccg cggccgttcg caacgctggc ggaacagctc ggcagcgacg
     2821 ccgacaccgt gcgtgccggc ttcgcggcga tgcaggcacg cggcgcgatc cggcgcatcg
     2881 gcgcgctgcc cgatcactac gcgctcggct acgtcgccaa cggcatgacg gtctgggaca
     2941 tcgacgatgc gcagcttcac gactgcggcc gccgcctcgg cgcgctcggc ttcgtcagcc
     3001 actgctacca ccgcccgcgc agcctgccgg cctggccgta caacctgttc gcgatggtgc
     3061 atgcgcacga ccgcgccgaa gtcgaggccc gccgcgccga aatgcgcgcg ctgctcggca
     3121 gcgcctgccg cgccgatgcc gtgctctaca gcacgcgcat cctgaagaag accggcctgc
     3181 gcatcggcgc ctga
//
LOCUS       DAOQIE010000388         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19792_length_3193_cov_7.724984, whole genome shotgun sequence.
ACCESSION   DAOQIE010000388 DAOQIE010000000
VERSION     DAOQIE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19792_length_3193_cov_7.724984"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1469
                     /locus_tag="PK144_17195"
     CDS             51..1469
                     /locus_tag="PK144_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNF67509.1"
                     /translation="MSDAVDPELRQQRGKALRKRASRKQHDHIGGHDRDPVALLEASS
                     RTRLPRLLPLRYGRMLASPFAFFRGSAIVQAHDLAGTPYSGLIVPLCGDCHLMNFGGF
                     ATPERNLIFDVNDFDETHPGPWEWDVKRLAASFHIAARHQGYAEHDCADIVAAALDSY
                     REHTKTYARCSELELWYDRISFERLIQEAEDPDMRAQLAQTVEKASGRTHEHLLPKLG
                     SRTGDHWTMHDAPPGLFHIHGPASLFEPDDDWRRPGDWQRLIQPMYAAYLDTVSSSHR
                     ALLGRFRAQDLAFKVVGVGSVGTRCLVLLLTDGHDSPLFLQFKEALPSVLADYVPAGR
                     CPYRHQGRRVVAGKRLMQASSDIFLGWTTAFDGRQFYVRQLRDMKVSPEIETYDEAQL
                     HYYAASCGRVLARGHARAGGLAPEIAGYLGNGDAYTAAVLDYARDYADRVEQDFEKFR
                     SACRSGRLQAQTEADLAAEFRV"
     gene            complement(1473..2249)
                     /locus_tag="PK144_17200"
     CDS             complement(1473..2249)
                     /locus_tag="PK144_17200"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HNF67510.1"
                     /translation="MNLHELQTAFAATLRGREPLAGLDAAVLAGSLSVAERLAIYRNN
                     HRLTLAGALVSAYPVLERLLGCASFAQLAARYVAQTPSLCGDLHGYGATFGVFLDDFT
                     PLADWPWHADLARLEWAVHEAYYAADAATVLSLAELAALPPERQQSLRLRARPSLRFT
                     ASRWPVLDLWVANRDDAAHAVDPEAGGQHLLIWRTAGEGGDIAIVPVTAGEWELAAAL
                     ACGAGLDTACEVAIAVDPDCAPGPALGRLLALGVFTGIAG"
     gene            complement(2236..3126)
                     /locus_tag="PK144_17205"
     CDS             complement(2236..3126)
                     /locus_tag="PK144_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNF67511.1"
                     /translation="MNDGAPVSGRQSAASMPAIAGIGLRGDHYEALLAGDFGDQAGPG
                     WVEVHSENYFGDGGRPLAVLDRVRAHWPVSLHGVGLGLGNADGLDRGHLARLQALVAR
                     VEPGWVSEHLCWTAAGGQHLNDLLPLPMSEEALALVCRHVQQVQEALGRPLLVENVSR
                     YLDFAASVIPEGEFLAELVARTGCGLLLDVNNLYVNHVNHGDDPLAIVAALPAAAIGE
                     IHLAGHSRREVRGQALLIDTHDAPVCAEVWALYRQVLARIGPRATLIERDSALPPLPP
                     LLAEAAIADACLGACRDEPA"
BASE COUNT          505 a         1154 c         1110 g          424 t
ORIGIN      
        1 ccctgattta ccctccgccc accagagccg tgccaccggg agtcccttcg atgagcgatg
       61 ccgttgatcc cgaactgcgc cagcaacgcg gcaaggccct gcgcaagcgg gcctcgcgca
      121 agcagcacga tcacatcggc gggcatgacc gcgatccggt cgcgctgctg gaggcgagca
      181 gccgcacgcg gctgccgcgg ctgctgccgc tgcgctacgg gcgcatgctc gcatcgccgt
      241 tcgcgttctt ccgcggctcg gcgatcgtgc aggcgcatga tctggccggc acgccgtaca
      301 gcggcctgat cgtgccgctc tgcggcgact gccacctgat gaacttcggc ggcttcgcca
      361 cgcccgagcg caacctgatc ttcgatgtca acgacttcga cgaaacccac cccggcccct
      421 gggaatggga cgtgaagcgc ctcgccgcca gcttccacat cgccgcccgc caccagggct
      481 acgccgaaca cgactgcgcc gacatcgtcg ctgccgccct cgacagctac cgcgaacaca
      541 cgaagaccta cgcgcgctgc agcgaactcg aactctggta cgaccgcatc agcttcgagc
      601 gcctgatcca ggaagccgaa gacccggaca tgcgcgcgca gctcgcacag acggtcgaga
      661 aagccagcgg gcgcacgcac gaacacctgc tgccgaagct cggcagcaga acgggcgatc
      721 actggacgat gcacgacgca ccgccgggcc tgttccacat ccacggcccg gccagcctgt
      781 tcgagcccga cgatgactgg cgccggcccg gcgactggca acggctgatc cagccgatgt
      841 acgcggccta tctcgatacg gtcagcagca gccaccgcgc gctgctcggg cgcttccgcg
      901 cgcaggatct cgccttcaag gtggtcggcg tcggcagcgt cggcacgcgc tgtctggtgc
      961 tgctgctgac cgacggccac gacagcccgc tgttcctgca gttcaaggag gcgctgccct
     1021 cggtgctggc ggactacgtg ccggccgggc gctgcccgta ccggcatcag ggccggcgcg
     1081 tggtcgccgg caagcgcctg atgcaggcat cgagcgacat ctttctcggc tggaccacgg
     1141 ccttcgacgg tcgccagttc tacgtgcgtc agttgcgcga catgaaggtg tccccggaga
     1201 tcgaaaccta cgacgaggca caactgcact actacgccgc gagctgcggc cgggtgctgg
     1261 cccgcggcca cgcccgcgcc ggcggcctcg ccccggagat cgccggctat ctcggcaacg
     1321 gcgatgcgta caccgcggcc gtgctcgact acgcgcgcga ctacgccgat cgcgtcgagc
     1381 aggacttcga gaaattccgc agcgcctgcc gcagcggccg gctgcaggcg cagaccgaag
     1441 ccgatctggc ggcggagttc cgggtgtagc ggtcaaccgg cgatgccggt gaacaccccg
     1501 agcgccagca gccggccgag cgccggaccg ggggcgcagt cgggatcgac ggcgatggcg
     1561 acctcgcagg cggtgtcgag gccggcgccg caggcgagcg cagcggcgag ttcccattcg
     1621 ccggccgtga ccggcacgat ggcgatgtcg ccgccttcac cggcggtgcg ccagatcagc
     1681 aggtgctggc cgccggcctc gggatcgacc gcgtgcgcgg catcgtcgcg gttcgcgacc
     1741 cagagatcga gcaccggcca gcgcgaggcg gtgaagcgca gcgacggccg ggcccgcagg
     1801 cgcagtgact gctgacgctc gggcggcagc gccgcgagtt cggcgagcga cagcaccgtc
     1861 gcggcatcgg cggcgtaata cgcctcatgc accgcccatt cgagccgcgc cagatcggca
     1921 tgccacggcc agtcggcgag cggcgtgaaa tcgtcgagga agacgccgaa cgtggcgccg
     1981 tagccgtgca ggtcgccaca gagcgagggc gtctgcgcga cgtagcgcgc cgcgagctgc
     2041 gcgaagctcg cgcagccgag caggcgctcc agcaccggat acgccgagac cagcgcgccg
     2101 gcgagcgtca gccggtggtt gttgcggtag atcgccagcc gctcggcgac gctcaggctg
     2161 ccggcgagca cggcggcgtc gaggccggcg agcggctcgc ggccccgcag ggtcgccgcg
     2221 aacgcggttt gaagctcatg caggttcatc acgacaggct ccgagacagg catcggcgat
     2281 cgcggcttcg gccagcagcg gcggcagcgg cggcagcgcg ctgtcgcgtt cgatcagcgt
     2341 cgcccgcggc ccgatgcgcg cgagcacctg ccggtacagt gcccagacct cggcgcagac
     2401 cggcgcatcg tgcgtgtcga tcagcagcgc ctgaccgcgc acctcgcgcc ggctgtgacc
     2461 ggccaggtga atctcgccga tcgccgccgc cggcagcgcc gcgacgatcg ccagcggatc
     2521 gtcgccgtgg ttgacgtggt tcacgtacag gttgttgacg tcgagcagca gcccgcagcc
     2581 ggtgcgcgcg accagctcgg cgagaaactc gccctcgggg atcaccgagg cggcgaagtc
     2641 gagatagcgc gagacgttct cgaccagcag cggccggccc agggcttcct gtacctgctg
     2701 cacgtgacgg cagacgaggg cgagggcttc ctcgctcatc ggcagcggca gcaggtcgtt
     2761 caggtgctgg cccccggcgg cggtccagca gagatgttcg gacacccacc ccggctcgac
     2821 gcgtgcgacc agcgcctgca gccgcgccag atgcccgcga tcgagaccat cggcgttgcc
     2881 gaggcccaga ccgacgccgt gcaggctgac gggccagtgc gcgcgcacgc gatcgagcac
     2941 ggcgagcggc cggccgccgt cgccgaagta gttctcgctg tggacctcga cccagccggg
     3001 accggcctga tcgccgaagt cgccggccag cagggcttcg tagtgatcgc cgcgcaggcc
     3061 gatgccggcg attgccggca tcgacgcagc actctggcga ccggataccg gcgcgccgtc
     3121 attcatggcg tcgctcacgc cttcttggtc aggctgccgc cggtgatctt ctcgcaggcg
     3181 cccttgggca gat
//
LOCUS       DAOQIE010000389         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19873_length_3185_cov_7.067412, whole genome shotgun sequence.
ACCESSION   DAOQIE010000389 DAOQIE010000000
VERSION     DAOQIE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19873_length_3185_cov_7.067412"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK144_17210"
     CDS             complement(<1..311)
                     /locus_tag="PK144_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915960.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNF67512.1"
                     /translation="MSESQCPFHHTSGGGTSNRDWWPNQLNLDILRQHAGKSSPTGAG
                     FDYAKAFAGLDYAALKQDLHALMTDSQDWWPADYGHYGPMFIRMAWHSAGTYRTGDGR
                     GG"
     gene            720..1142
                     /locus_tag="PK144_17215"
     CDS             720..1142
                     /locus_tag="PK144_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF67513.1"
                     /translation="MHAHLTMITLAVEDLERAVRFYRDGLGLPTDGIVGSEFEHGAVA
                     FFELAGGLALALWPRASLAHETGSVAGTPGTTDFALAHNVTTIEEVDALLAQAQAAGA
                     QIVRPGAPAFWGGYTGWFRDPDGHLWEIAWNPSRLPQA"
     gene            complement(1153..2094)
                     /locus_tag="PK144_17220"
     CDS             complement(1153..2094)
                     /locus_tag="PK144_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF67514.1"
                     /translation="METGKTSGGLPPDFEGLHACIDHLPGNVMLADAGFILRYANRAA
                     LRTLRLLEPEIQRVFGVRFEQLLNGSIHRFHREPERIERLLRDYAQTLPREASFTFGS
                     VTLRTHINAVLSDGRLLGYCVIWDDISRESALEAEIALSRERAGQLAADLLHRLSELR
                     LSVEEIARTCTQVVEVATRGRVASDLAASSVGRLGQQGEAIGGVLDLIGKVAEQTKLL
                     ALNAGIEASRAGEAGRGFAVVAREIKDLAAATAGATGDIAKRIGTIRDGVEVTVEAIE
                     ALRRIIDELHHLQTQVAAAVEEQSAVVAELERARLQP"
     gene            2513..3169
                     /locus_tag="PK144_17225"
     CDS             2513..3169
                     /locus_tag="PK144_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNF67515.1"
                     /translation="MNWYGCAQPGADLGNIDLRGAVLEYTDFSAARFAGAQLEDADFG
                     RSRLAAADFTRATLSNARFVAATLDAANFSGARLDGARLERAQAAGANFTGADLSAAN
                     AYQAQLGKAVFDGARLDGARLEQAVLDDARFDGASLRSARLQRASAERTRFTGADLAG
                     ARLDGARLDGADFSGADLTGASLVGARIDGARFDGARLGDCIWTDRRRCRPGSVGQCD
                     "
BASE COUNT          441 a         1166 c         1057 g          521 t
ORIGIN      
        1 ccgccgcggc cgtcgccggt gcggtaggtg ccggcgctgt gccaggccat gcggatgaac
       61 atcggtccgt aatggccgta atccgccggc caccagtcct gcgaatcggt catcagcgcg
      121 tgcaggtcct gcttcagggc cgcgtaatcc agaccggcga aggccttggc gtagtcgaaa
      181 ccggccccgg tcggactgga cttgccggcg tgctggcgca ggatgtccag attcagctga
      241 ttgggccacc agtcacgatt cgacgtgccg ccaccggagg tgtgatggaa cgggcattgg
      301 ctttcggaca tctcgtcatc tccacttgcg ttctggtttc gtactgcggg tccggcccgg
      361 tctgtatagc cgcccgccca ccggtgcgac caattgaacc tgccaaagcc cgcgatagcc
      421 tgccgcgatg gttgatgcgg gatgacgccc gaatgccgct gcggaccgca gcgatggatc
      481 acggcagatg ccggttccgg taataccttc cgtccgatgc cgattgccgg cacggccggg
      541 tttcagaatt cctgcatttc accgcgaaaa cttcatgctt tccggccgcc aatcccgtcc
      601 ggccggctga aaacggcttc tgcgcgcagg accggcatgc ggattctccc ggcatgacga
      661 cgcacgtcct gccatattca gaggtcgagg gctgcagacc ccgaacggag accgccgcca
      721 tgcatgcaca cctgacgatg atcacgctgg ccgtcgagga tctcgaacgc gccgtgcggt
      781 tctaccgcga cggcctcggc ctgccgacgg acggcatcgt cggcagcgaa ttcgagcacg
      841 gtgccgtcgc gttctttgag ctggccggcg gcctggcgct ggcgctgtgg ccgcgcgcga
      901 gtctggccca tgaaaccggc agcgtcgccg gaacgcccgg cacgacggat ttcgcgctcg
      961 cgcacaacgt cacgaccatc gaggaggtcg atgcgctgct cgcgcaggcg caggcggccg
     1021 gcgcgcagat cgtccggccg ggcgcgccgg ccttctgggg cggttacacc ggctggttcc
     1081 gcgatccgga cgggcatctg tgggagatcg cgtggaaccc gtcacggctg ccgcaggcct
     1141 gatccgcccg gctcacggct gcaggcgcgc gcgctccagc tcggcgacca ccgccgactg
     1201 ctcctcgacc gcggctgcca cctgcgtctg caggtgatgc agctcgtcga tgatccggcg
     1261 cagcgcctcg atcgcttcga cggtcacctc gacgccgtca cggatcgtgc cgatgcgctt
     1321 ggcgatgtcg ccggtggcgc cggcggtcgc ggcggcaaga tccttgatct cgcgcgcgac
     1381 caccgcgaag ccgcgtccgg cctcgccggc gcgcgaagcc tcgatgccgg cgttgagcgc
     1441 cagcagcttg gtctgctcgg cgaccttgcc gatcaggtcg agcacgcccc cgatcgcctc
     1501 gccctgctgg ccgagccgcc cgaccgaact ggcggccagg tcgctggcca cgcgcccgcg
     1561 ggtcgcgacc tcgacgacct gcgtgcaggt gcgtgcgatc tcctcgacgc tgaggcgcag
     1621 ctcggagagc cggtgcagca ggtcggcggc gagctggccg gcgcgttcac gactcagggc
     1681 gatttccgcc tccagcgcgg attcgcggct gatgtcgtcc cagatcacgc agtaccccag
     1741 cagccggccg tcgctgagca cggcattgat gtgcgtgcgc agcgtgacgc tgccgaacgt
     1801 gaagctcgcc tcgcgcggca gcgtctgcgc gtagtcgcgc agcagccgct cgatgcgctc
     1861 gggctcgcgg tggaagcgat ggatcgagcc gttcagcaac tgctcgaagc gcacgccgaa
     1921 gacccgctgg atttcaggtt cgagcagccg cagcgtgcgc agcgccgcgc ggttggcata
     1981 gcgcagaatg aaaccggcat ccgccagcat gacgttgccg ggcaggtgat cgatgcaggc
     2041 atgcagcccc tcgaaatccg gaggcagccc tccggaggtc tttccggttt ccatgtcgta
     2101 taccccttct cgctttattg gtctggcctg tatggctggc ccggcctgtc cggccggacc
     2161 tgcccgtgcg tctggaaacc agcctccgcc ggcccggatg cacgatgtgg acggcaccgg
     2221 tcgctccggt cgccgctgat catgttcccc tgccggcccg gggccgtgca gcctgttacc
     2281 gccatttcat cgcacccgca tcggcgcgca cgccggcgac gcttcgcgtt aggctgcgcg
     2341 accgatccgc ccgccaccgt gtgcccgagc catgtttgcg tgcccgcagc ctgtcctgac
     2401 cgtcccgccc ccgtcccggc ccgccggcgt actgccgtgc gtcgtcgcgc ttgcacttgc
     2461 gctgactgcc ggcatcgcgt ccgcggcctg caccgatgcg ccggcccggc gcgtgaactg
     2521 gtacggctgt gcgcaaccgg gagccgatct cggcaatatc gacctgcgcg gcgcagttct
     2581 ggagtacacc gacttcagcg ccgcccgctt cgccggtgcg cagctcgaag atgccgattt
     2641 cggccgcagc cgcctcgccg ccgccgattt caccagggcc acgctcagca acgcccgctt
     2701 cgtcgcggcc acgctcgatg ctgcgaactt cagcggcgca cggctcgacg gcgcccggct
     2761 cgaacgcgcg caggccgccg gcgcgaactt caccggtgcc gatctcagcg ctgccaacgc
     2821 ctatcaggcc cagctcggca aggccgtgtt cgatggcgcc cgcctcgacg gcgcccggct
     2881 cgaacaagcc gtgctcgatg acgcacgctt cgacggcgcc agcctgcgtt ccgcccgcct
     2941 gcagcgtgcc agcgccgagc gcacccgctt caccggcgcc gatctcgccg gtgcgcgcct
     3001 cgatggcgcc cggctcgacg gcgccgattt ctccggggcc gatctgaccg gcgcctcgct
     3061 ggtcggcgca cgcatcgacg gcgcacgctt cgacggcgcc agactcggcg actgcatctg
     3121 gaccgatcgc cgccgttgcc gtcccggctc ggtcggtcag tgcgactgag cgcccctgcc
     3181 ccgga
//
LOCUS       DAOQIE010000390         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19945_length_3178_cov_6.570925, whole genome shotgun sequence.
ACCESSION   DAOQIE010000390 DAOQIE010000000
VERSION     DAOQIE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19945_length_3178_cov_6.570925"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PK144_17230"
     CDS             complement(<1..641)
                     /locus_tag="PK144_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF67516.1"
                     /translation="MNAPEPVLPVSGPTVGRRLAAAREQRGLDQAAAARLSGLSAEVV
                     RGLEAEDWAALGAPVYVRGWLARYCRVLDVEASSALAEYRQAVPAEPPPLRLRGEAPS
                     SAPRGHHSLRPFAYLLSFALLGYVGWQAFEKLSTDLTVSGNAGLAQPTGVARAPGGLT
                     RSESARPAPVTASAPETTPAPAPAAAPAAVNPVAATPGHAAAPAPSMNPVAPAT"
     gene            complement(638..1456)
                     /locus_tag="PK144_17235"
     CDS             complement(638..1456)
                     /locus_tag="PK144_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67517.1"
                     /translation="MRTGLRIVCVLGAVAMLGGCASWFGDGGDKEKNLATTTNVRAPS
                     TDPFEIDKKQAAQLNLQLGVGYIRQGDYQVAVSKLRKSLSFDDDSAEAHNALGIALEE
                     LRSYDEAAREYDRALALKSGYTAALINRTRLQCVRGTQAAEGEQRFIELAGSGTLEAP
                     ETAWIEAASCARRQQAWARAGAHLDRALEVAPGAMAQVLYERALLAFEQRNLPQAREL
                     LRRLHGDYGSSARSLGLAVRVEDALGGSAQRRQLVAELLRAYPDSPEARSYRPR"
     gene            complement(1453..2598)
                     /gene="rlmN"
                     /locus_tag="PK144_17240"
     CDS             complement(1453..2598)
                     /gene="rlmN"
                     /locus_tag="PK144_17240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNF67518.1"
                     /translation="MPVDAPARTNLLGLTRPELEAFMVSLGEKPFRARQLMKWIYHEG
                     VADFDAMTDLAKKFRARLAEVAEVRAPEVVLDQASGDGSHKWLIRVDSGNCIESVFIP
                     ETDRGTLCVSSQIGCALDCSFCSTAQQGFNRNLSVAEIVGQVWAANHALGWGSNDTRI
                     ITNVVMMGMGEPLANFANVVAAMQIMLDDFGFGLSKRRVTLSTSGIVPALYRLAEVTD
                     VSLAVSLHAPDDALRDELVPINRKYPIAELLAACAHYISDKPHRRVTWEYVMLDGVND
                     SDAHAHALAQLIRHIPSKINLIPFNPFPGTRYRRSPQARIDRFRDILIGGYGITTVTR
                     RTRGDDIDAACGQLAGRVQDRSRRVTQVVAIEPAVRGAGTAFGQPSA"
     gene            complement(2634..3065)
                     /gene="ndk"
                     /locus_tag="PK144_17245"
     CDS             complement(2634..3065)
                     /gene="ndk"
                     /locus_tag="PK144_17245"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNF67519.1"
                     /translation="MAIERTLSIIKPDAVAKNLIGAIYARFEAAGLKIVAAKMAHLSQ
                     EQAEGFYAVHRERPFFKDLVSFMISGPVMIQVLEGENAIQKNREIMGATDPKKAAAGT
                     IRHDFAVSVDENAAHGSDAAETAAVEIAYFFGQDGVCPRTR"
BASE COUNT          454 a         1086 c         1163 g          475 t
ORIGIN      
        1 gtggccgggg ccaccgggtt catcgacggc gccggcgcag ccgcatgccc gggcgtggcg
       61 gccaccggat tcaccgctgc cggcgcggca gccggtgccg gcgccggtgt ggtctcgggg
      121 gcgctggcgg tcaccggcgc cgggcgcgcg gattcgctgc gcgtcagccc gcccggggcg
      181 cgggcgaccc cggtcggctg cgcgagaccg gcgttgccgc tgacggtcag atcggtcgac
      241 agcttctcga aggcctgcca gccgacgtag ccgagcagcg cgaagctcag caggtatgca
      301 aacggccgca gcgaatgatg gccgcgcggg gcggaactcg gggcctcgcc acgcaggcgc
      361 agcggcggcg gctcggccgg caccgcctga cggtattcgg cgagcgccga cgaggcttcg
      421 acatcgagca cgcggcagta gcgggccagc cagccgcgca cgtagaccgg cgccccgagc
      481 gcggcccagt cctcggcctc caggccgcgc acgacctcgg cactcagccc gctgaggcgc
      541 gccgcagcgg cctgatccag gccgcgctgc tcgcgggccg cagcaagacg ccggccgacg
      601 gtcggtccgg atacgggaag gacgggctcg ggtgcgttca tcgcggacgg taagaccggg
      661 cttcgggcga atcgggatag gcacgcagca gctcggcgac gagctggcga cgctgcgcac
      721 tgccgccgag cgcgtcctcg acgcgcacgg cgaggccgag gctgcgcgcg ctgctgccgt
      781 aatcgccgtg cagccggcgc agcagctcgc gggcctgcgg caggttgcgc tgctcgaagg
      841 cgagcagcgc acgctcgtac agcacctggg ccatcgcgcc cggtgccacc tcgagcgcgc
      901 gatcgagatg cgcgccggcc cgcgcccagg cctgctggcg ccgggcacag ctggcggcct
      961 cgatccaggc ggtttccgga gcttcgaggg tgccggaacc ggccagctcg atgaagcgct
     1021 gctcgccttc ggccgcctgc gtgccgcgca cgcactgcag gcgcgtgcgg ttgatcagcg
     1081 ccgcggtata gccggatttc agcgccagcg cccggtcgta ctcgcgggcg gcttcgtcat
     1141 agctgcgcag ctcttcgagc gcgatgccga gcgcattgtg cgcctcggcc gaatcgtcgt
     1201 cgaaactcag cgacttgcgc agcttgctca cggcgacctg gtagtccccc tgacggatgt
     1261 agccgacacc gagctgcagg ttcagctgcg cggcctgctt cttgtcgatc tcgaaggggt
     1321 cggtgctcgg cgcccggacg ttcgtggtcg tggcgaggtt cttttccttg tcgccaccgt
     1381 cgccgaacca cgaggcgcag ccaccgagca tggcgacggc gcccagcacg cagacgatgc
     1441 gcaggcccgt cctcatgccg acggctgtcc gaacgcagtc cccgccccac gcacggccgg
     1501 ctcgatcgcc accacctgcg tgacgcggcg gctgcgatcc tgcacgcgcc cggcgagctg
     1561 gccgcaggcg gcgtcgatgt cgtcgccgcg cgtgcggcgc gtgacggtgg tgatgccgta
     1621 gccgccgatc aggatgtcgc ggaagcggtc gatgcgcgcc tgcggcgagc gccggtagcg
     1681 cgtgcccggg aacgggttga acgggatcag gttgatcttc gacgggatgt gccggatcag
     1741 ctgcgcgagc gcgtgggcgt gcgcatcgga gtcgttgacg ccgtcgagca tcacgtactc
     1801 ccaggtcacg cgccggtgcg gcttgtcgct gatgtagtgc gcgcaggccg cgagcagctc
     1861 ggcgatcggg tacttgcggt tgatcggcac gagttcatcg cgcagcgcat cgtcgggcgc
     1921 gtgcagcgac acggcgaggc tcacgtcggt gacctcggcg agccggtaca gcgccggcac
     1981 gatgcccgag gtcgacagcg tcacgcgccg cttcgacagg ccgaagccga agtcatcgag
     2041 catgatctgc atcgccgcga ccacgttcgc gaagttcgcg agcggctcgc ccatgcccat
     2101 catcacgacg ttggtgatga tgcgcgtgtc gttgctgccc cagccgagcg cgtggttggc
     2161 ggcccagacc tgaccgacga tctcggcgac cgacaggttg cggttgaagc cctgctgcgc
     2221 ggtcgagcag aagctgcagt cgagcgcgca gccgatctgc gaggacacgc acagcgtgcc
     2281 gcggtcggtc tccgggatga acaccgattc gatgcagttg ccgctgtcga cgcggatcag
     2341 ccacttgtgc gaaccgtcgc ccgaggcctg gtcgagcacg acctcgggtg cgcgcacctc
     2401 ggcgacctcg gcgaggcgcg cgcggaactt cttcgcgagg tcggtcatcg cgtcgaagtc
     2461 ggcgacgccc tcgtggtaga tccacttcat cagctgccgg gcgcggaacg gcttttcccc
     2521 gagcgagacc atgaaggcct ccagttcggg gcgcgtcagg cccagcaggt tggtgcgcgc
     2581 gggggcgtcg accggcatcg tcacgtcagg caaccgtcga tcaggcacgc ggatcagcgc
     2641 gtgcgcgggc agacgccgtc ctggccgaag aagtaggcga tctcgacggc cgcggtctct
     2701 gcggcgtcgg agccgtgcgc ggcgttctcg tcgaccgaga cggcgaagtc gtggcggatc
     2761 gtgccggcgg cggccttctt cgggtcggtc gcgcccatga tctcgcggtt cttctggatc
     2821 gcgttctcgc cttcgagcac ctggatcatc accgggccgg agatcatgaa gctgaccaga
     2881 tccttgaaga acggccgctc ccggtgcacc gcgtagaagc cttcggcctg ttcctgcgac
     2941 aggtgcgcca tcttcgccgc gacgatcttc aggccggcgg cctcgaagcg cgcatagatc
     3001 gcgccgatca gattcttggc gacggcgtcc ggcttgatga tggacagggt gcgctcgatg
     3061 gccattcggt gaaactccgt tgcggttcag aaacttgggt ggggaaatac aatgccgccc
     3121 gcggccggtc ggcgcgcggg cggcaagtgt accggctgca ccgggcagcg gcaacgcg
//
LOCUS       DAOQIE010000391         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_19957_length_3177_cov_9.097694, whole genome shotgun sequence.
ACCESSION   DAOQIE010000391 DAOQIE010000000
VERSION     DAOQIE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19957_length_3177_cov_9.097694"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK144_17250"
     CDS             complement(<1..457)
                     /locus_tag="PK144_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015270793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF67520.1"
                     /translation="MNAQTSLNRDLTEDLRRRVLDAAETRTPLAIRGSGSKEFYGRGV
                     RGEPLDVSGHRGIVSYEPTELYLTARAGTPLAEIDAALTDSGQMLGCEPPAFGPGATV
                     GGTVAAGLSGPRRPYAGSVRDYVLGVKVLNGRGEVLRFGGQVMKNVAGYD"
     gene            complement(496..1959)
                     /locus_tag="PK144_17255"
     CDS             complement(496..1959)
                     /locus_tag="PK144_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF67521.1"
                     /translation="MTDDATRALRDAIARGVPERLILADIEDLRPYECDGLTAYRTVP
                     RLVVLPETAEQAAHVLATCHERGVPVVARGAGTGLSGGAMPHADGVVLSLAKFNRILG
                     IDPQNRTARVQPGVRNIAISQAAAPLGLYYAPDPSSQIACTIGGNVAENAGGVHCLKY
                     GLTVHNIVALDVLTITGERLTIGAGALDQPGYDLLALLTGSEGMLAVIVEVTVKLLPK
                     PQVAQVVMGVYDRVDAAGVAVGRIIAAGITPGGLEMMDNGCIRAAEDYLHAGYPVEAA
                     AILLCELDGTAEEVAEDLARVEALMRDAGASDIRISQSEEERLRFWAGRKAAFPAVGR
                     ISPDYYCMDGTIPRKELPNVLNRIEALSQRYGLPVVNVFHAGDGNLHPLILFNGGDPA
                     QLHRAEQLGGEILELCVEVGGAITGEHGVGFEKINQMCSQFAKDELVQFHALKAAFDP
                     KGLLNPGKAVPTLARCAEYGAMRISHGELPFPELERF"
     gene            2270..3043
                     /locus_tag="PK144_17260"
     CDS             2270..3043
                     /locus_tag="PK144_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNF67522.1"
                     /translation="MQTTSSIQVIDRLARLLDAIAANNNPVSLKVLSAETGLHPSTAF
                     RILASLAEHGFVERSSSGHYRLGVKLLQLGSRVQGRLDIRREARPIMEWLRNETGETV
                     NLIVREGDEVVYVERVVPNRMMRVEQMIGGRAPLHVTAVGKLFLAEGGAEACLEYAAR
                     TGLPSCTPHSITDPTALWRSVKNALQQGYALDDQEAELGVGCIGVPIRDSTNHIVAGI
                     SVSAPIERRREVWVTLIRQAGEKLSTRLGFHPENRPVGA"
BASE COUNT          493 a         1083 c         1072 g          529 t
ORIGIN      
        1 cgtcgtagcc ggcgacgttc ttcatgacct ggccgccgaa gcgcagcacc tcgccgcggc
       61 cgttgagcac cttgacgccg agcacgtagt cacgcaccga gccggcatag ggccggcgcg
      121 gtccggacag gccggcggcg accgtgccgc cgacggtcgc gcccggcccg aaggccggcg
      181 gctcgcagcc gagcatctgg ccgctgtcgg tgagcgccgc atcgatctcg gcgagcggcg
      241 tgccggcacg cgcggtcagg tacagctcgg tcggctcgta gctgacgatg ccgcgatggc
      301 cgctgacatc gagcggctcg ccccgcacgc cgcggccgta gaactccttg ctgccactgc
      361 cgcggatggc gagcggcgtg cgcgtctcgg cggcgtcgag cacgcgccgg cgcaggtctt
      421 cggtcaaatc ccggttgaga ctggtttgcg cattcatgga tcgtgtgttc tctcgttcgt
      481 ggccggacag cgcgctcaga agcgctccag ttccgggaac ggcagttcgc cgtggctgat
      541 gcgcatcgcg ccgtactcgg cgcagcgcgc cagcgtcggc acggccttgc cggggttcag
      601 caggcccttg gggtcgaatg cggccttcag cgcgtggaac tgcacgagtt cgtccttcgc
      661 gaactgcgaa cacatctggt tgatcttctc gaagccgacg ccgtgctcgc cggtgatcgc
      721 cccgccgacc tcgacgcaga gttcgaggat ctcgccgccg agctgctcgg cccggtgcag
      781 ttgcgccgga tcgccgccgt tgaacaggat cagcggatgc aggttgccgt cgccggcgtg
      841 gaagacgttc acgaccggca ggccgtagcg ctggctcagc gcctcgatgc ggttcagcac
      901 gttcggcagc tccttgcgcg ggatcgtgcc gtccatgcag tagtagtccg gcgagatgcg
      961 cccgaccgcc gggaacgccg ccttgcgtcc ggcccagaag cgcaggcgct cttcctcgct
     1021 ctgcgaaata cggatgtcgc ttgcacccgc atcgcgcatc agcgcctcga cgcgcgccag
     1081 gtcctcggcg acctcctcgg ccgtgccgtc gagttcgcac agcaggatcg ccgcagcctc
     1141 gaccgggtag ccggcgtgca ggtagtcctc ggcggcgcgg atgcagccgt tgtccatcat
     1201 ctccagcccg cccggcgtga tgccggcggc gatgatgcgc ccgaccgcga cgccggcggc
     1261 gtcgacgcgg tcgtagacgc ccatgaccac ctgcgcgacc tgcggcttcg gcagcagctt
     1321 gaccgtgacc tcgacgatga ccgcgagcat gccctcggag ccggtcagca gcgccagcag
     1381 gtcgtagccg ggctgatcga gcgcgccggc cccgatcgtc aggcgctcgc cggtgatggt
     1441 cagcacgtcg agcgcgacga tgttgtgcac ggtcagcccg tatttcaggc agtgcacgcc
     1501 gccggcgttc tcggcgacgt tgccgccgat cgtgcaggcg atctgcgagg acgggtccgg
     1561 cgcgtaataa agccccagcg gcgcggcggc ctgcgaaatc gcgatgttgc gcacgcccgg
     1621 ctgcacgcgc gcggtacggt tctgcgggtc gatgccgagg atgcggttga acttggcgag
     1681 cgacagcacg acgccgtcgg catgcggcat cgcgccgccc gagagcccgg tgccggcccc
     1741 gcgcgcgacc accggcacgc cgcgctcgtg gcaggtcgcg agcacgtgcg cggcctgttc
     1801 ggcggtctcc ggcagcacca cgaggcgcgg cacggtgcgg taggcggtca gaccatcgca
     1861 ctcgtagggg cgcaggtcct cgatatcggc caggatcagc cgttcgggca caccgcgggc
     1921 tatcgcgtcg cgcaatgcgc gggtcgcgtc gtcagtcatt ggtcttacct ctcatctccc
     1981 gaccggatgc gccggccgcg ggctcctggt gtggcttcaa ccctgcattc cctgcggagt
     2041 ttatgtgcat tctgcgcccg actgtttacc gcagtccggg aaaaaacgcc agccatctgg
     2101 atcaatggcc agaccaaagc agaaattggc ctgtccgcat tcgcatcgtg aaaacatctt
     2161 catgccccgc atcaggagta gcaatttttt gtgcagtgca atagactatt tttcgtgatg
     2221 caaaacgtct ttactcgatc agcaatgcgc gtggagtttc ggaatggtca tgcagacgac
     2281 ctcgtccatt caggtcattg atcggctcgc ccggctgctg gatgcaatcg ccgccaacaa
     2341 caatccggtc agcctcaagg tgttgtcggc cgagaccggc ctgcatccat cgacagcctt
     2401 ccgcatcctc gcctcgctgg ccgagcacgg gttcgtcgaa cgtagttcct cgggccacta
     2461 ccgtctgggc gtgaagctgc tgcagctcgg ctcgcgcgtg cagggccggc tcgacatccg
     2521 ccgcgaggcg cgcccgatca tggaatggct gcgcaacgag accggcgaga cggtcaacct
     2581 gatcgtgcgc gagggcgacg aggtcgtgta cgtcgagcgc gtggtgccga accgcatgat
     2641 gcgcgtcgag cagatgatcg gcggccgggc gccgctgcac gtcaccgcgg tcggcaagct
     2701 gttcctcgcc gagggcggcg ccgaagcctg tctcgaatac gccgcgcgca ccggcctgcc
     2761 ctcgtgcacg ccgcactcga tcaccgaccc gacggcgctg tggcgctcgg tcaagaacgc
     2821 gctgcagcag ggctacgcgc tcgatgacca ggaagccgaa ctcggcgtcg gctgcatcgg
     2881 tgtgccgatc cgcgactcga cgaaccacat cgtcgccggc atctcggtct cggccccgat
     2941 cgaacgccgc cgcgaggtct gggtgacgct gatccgtcag gccggcgaga agctgtcgac
     3001 gcggctcgga ttccatccgg agaaccggcc tgtaggcgct taaccaatac aaaacaacag
     3061 cttgatgcgg cgcaactact gaatatcccg aagtggtgcc cgcagtggta cctgaattca
     3121 gcgcgctgca gcggtacgcg acgggacagg acgggtgccg tcgttgctgg ccggtgc
//
LOCUS       DAOQIE010000392         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_20193_length_3155_cov_7.195806, whole genome shotgun sequence.
ACCESSION   DAOQIE010000392 DAOQIE010000000
VERSION     DAOQIE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20193_length_3155_cov_7.195806"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK144_17265"
     CDS             complement(<1..644)
                     /locus_tag="PK144_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNF67523.1"
                     /translation="MNWWGKMVGGAFGFMVGGPFGAMFGAAVGHNFDRNAARFAGLHD
                     GHGHGQGRHGSDAEAEQRFLIALFAGLGAVAKADGAVSAAELEAARAVIGHLRLDGLR
                     REQAMRAFNDGKRADYPLDATLDAFAEVLPQRPELLRAFLEIQLATAWAIGEPHPRAR
                     ECVGSMAARLGVGRGQLDALELMIRVQRGGFGGPGQGRQQHSGGHQHRQPARPAT"
     gene            complement(734..1396)
                     /locus_tag="PK144_17270"
     CDS             complement(734..1396)
                     /locus_tag="PK144_17270"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNF67524.1"
                     /translation="MNFPSELLPFELRLLADALLVAGCLVALYCAPWRALRRNAMEHV
                     LGAALVSLLLLWSFHAGVSPGLGFHFLGVTVLTLMFGWALALLGTVVIGIGMALFARL
                     DWAALAATILLIGVLPVLVSHGIQRLVTQRLPANPFIYIFLCGFFGAILSALCAVMAV
                     VATLVALDVYPFERIAREYLPFLPLYLFPEGLMNGMLTAVFVGMKPEWLRSFDESRYF
                     RS"
     gene            1609..2508
                     /locus_tag="PK144_17275"
     CDS             1609..2508
                     /locus_tag="PK144_17275"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNF67525.1"
                     /translation="MSDILESTPAELTSLPLIYRGKVRDVYGIDDRHILIVATDRLSA
                     FDVVLPTGIPGKGAMLTAISNFWFERTRQIVPNHLDLATVTLEQALPDPAERARVEGR
                     AVVARKLRGLPVEAIVRGYLIGSGWKDYRTSGAVCGIALPAGLRLAERLPEPIFTPST
                     KAAVGDHDENVSYEAVVAQIGAGLAAQVRDTSLRLYREAAAWALTRGIIIADTKFEFG
                     LDEHGALVLMDEALTPDSSRFWPADQYRAGSNPPSFDKQFVRDYLETLDWNKQAPGPA
                     LPEEILERSAAKYREAMTRLTGL"
     gene            2680..>3155
                     /locus_tag="PK144_17280"
     CDS             2680..>3155
                     /locus_tag="PK144_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67526.1"
                     /translation="MNSDREWLQTCLYGQHDIWADWVRFGERIADTQEAAGWGTPHAL
                     RLLNEQNARWLEATRLWVQEWERMVSAGPDFDYSARQALVRNRIFQTWLDTVSGFDWD
                     QWHLSMRQAFLLLQQAFQAAQVLQQQTPAPAEAPAPDTPDATTLNAGAPAPVLDPQP"
BASE COUNT          524 a         1146 c         1083 g          402 t
ORIGIN      
        1 gtggccgggc gcgccggctg gcggtgctgg tggccgccgg aatgctgctg ccggccctgg
       61 cccgggccgc cgaagccgcc gcgctgcacg cggatcatca gctccagcgc atcgagctgg
      121 ccgcggccga cgccgaggcg cgccgccatc gagccgacgc actcgcgcgc gcgcgggtgc
      181 ggttcgccga tcgcccaggc ggtcgcgagc tggatttcga ggaaggcccg cagcagctcg
      241 ggccgctgcg gcagcacctc ggcgaaggcg tcgagcgtcg catcgagcgg gtagtcggcg
      301 cgcttgccgt cgttgaacgc gcgcatcgcc tgctcgcggc gcaggccgtc gaggcgcagg
      361 tgaccgatca ccgcgcgcgc ggcttcaagc tcggcggcgc tgaccgcacc gtcggccttg
      421 gcgacggcgc cgagcccggc gaacagcgcg atcaggaagc gctgctcggc ctcggcatcg
      481 ctgccgtgac ggccctgacc gtggccgtgg ccgtcgtgca ggccggcgaa gcgtgcggcg
      541 ttgcggtcga agttgtggcc gaccgcggcc ccgaacatcg caccgaacgg tccgccgacc
      601 atgaagccga aggcgccgcc gaccatcttg ccccaccagt tcatgcgcac accccggcgc
      661 acggcaggga tgcaggggac gggaacggca ggtggtggct cggcatcggg gcggacacgg
      721 acgtgcaggg gtttcaggag cggaagtagc gcgattcgtc gaagctgcgc agccactcgg
      781 gcttcatgcc gacgaacacc gcggtcagca tgccgttcat cagcccttcc gggaacagat
      841 acagcggcag gaatggcaaa tattcacggg cgatgcgctc gaacggatac acgtcgagcg
      901 ccaccagcgt cgcgaccacg gccatgaccg cgcacagcgc cgagaggatc gcgccgaaga
      961 agccacacag aaagatataa atgaacgggt tggccggcag ccgctgcgtg accagccgct
     1021 ggatgccgtg gctgaccagc accggcagca cgccgatcag caggatcgtc gccgccagcg
     1081 cagcccagtc cagccgtgcg aacaaggcca tgccgatgcc gatcacgacc gtgccgagca
     1141 gcgccagcgc ccagccgaac atcagcgtca gcaccgtcac gccgaggaag tgaaagccga
     1201 ggcccggcga cacgccggcg tggaaggacc acagcagcag cagcgacacc agcgccgccc
     1261 cgagcacgtg ctccatggcg ttgcgccgca gcgcccgcca cggcgcgcag tacagcgcca
     1321 cgaggcagcc ggcaacgagc agggcatcgg cgagcaggcg cagctcgaac ggcagcagtt
     1381 cggacggaaa attcatggaa gcacctgcct ggccggatgg ccgatctgca cggcacgcca
     1441 gcgacgcgcc ttccccccga tggatggacc ggcgcccgcg acccgaattc cggggcggcc
     1501 cgtgcaccgt cccggacgcc acgccggccg gcggtgtgct tggcaccccg acggcggcgc
     1561 gcgcacaatc cgcgccctgt tccgcccgcc ccgaccggag tccgcgccgt gagcgacatc
     1621 ctcgaatcca ccccggccga gctgaccagc ctgccgctga tctaccgcgg caaggtccgc
     1681 gatgtctacg gcatcgatga ccggcacatc ctgatcgtcg ccaccgaccg gctgtcggcc
     1741 ttcgacgtgg tgctgccgac cggcatcccc ggcaagggcg cgatgctgac ggcgatctcg
     1801 aacttctggt tcgaacgcac gcgccagatc gtgcccaatc atctcgacct cgccaccgtc
     1861 acgctcgaac aggccctgcc cgacccggcc gaacgcgccc gcgtcgaggg ccgcgccgtg
     1921 gtcgcgcgca agctgcgcgg gctgccggtc gaggcgatcg tgcgcggcta cctgatcggc
     1981 tcgggctgga aggactaccg cacgagcggc gcggtctgcg gcatcgcgct gccggccggg
     2041 ctcaggctcg ccgagcggct gccggagccg atcttcacgc cctcgaccaa ggccgcggtc
     2101 ggcgatcacg acgagaacgt ctcctacgag gccgtcgtcg cgcagatcgg cgccgggctg
     2161 gccgcgcagg tgcgcgacac cagcctgcgc ctgtaccgcg aggccgctgc ctgggcgctg
     2221 acccgcggca tcatcatcgc cgacaccaag ttcgagttcg gtctcgacga gcacggcgcc
     2281 ctggtgctga tggacgaggc gctgacgccg gattcctcgc gcttctggcc ggccgaccag
     2341 taccgcgccg gcagcaatcc gccgagtttc gacaagcagt tcgtgcgcga ttacctcgaa
     2401 accctggact ggaacaagca ggccccggga ccggcgctgc ccgaggaaat cctcgaacgc
     2461 tcggccgcca agtaccgcga ggcgatgacg cgcctgaccg ggctgtgagc cccggagcgg
     2521 ccttgccgcg caccggcttc gattaaccga ccgtaaggtg ttgttacagg ccagttctct
     2581 gacaatcatc gccgtccacg ccgtgccggc tcccgccggc acggccatcg atccgggccc
     2641 agatccggac gctttcgatc caccgaggag cgcatcacga tgaacagcga tcgggaatgg
     2701 ttgcagacct gtctgtacgg acagcacgac atctgggcgg actgggtccg cttcggcgaa
     2761 cgcatcgccg acacccagga ggctgccggc tggggcacgc cgcacgcgct gcggctgctg
     2821 aacgagcaga acgcgcgctg gctggaagcc acgcggctgt gggtgcagga atgggagcgc
     2881 atggtcagcg ccggtccgga cttcgactac agcgcgcgtc aggcgctggt gcgcaaccgc
     2941 atcttccaga cctggctgga taccgtcagc ggcttcgact gggaccagtg gcacctgtcg
     3001 atgcgtcagg ccttcctgct gctgcagcag gccttccagg ccgcgcaggt gctgcagcag
     3061 cagacgccgg cgccggccga agctccggct cccgacactc cggacgccac gaccctgaac
     3121 gctggcgccc cggcgccggt cctcgatccg cagcc
//
LOCUS       DAOQIE010000393         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_20530_length_3125_cov_7.493160, whole genome shotgun sequence.
ACCESSION   DAOQIE010000393 DAOQIE010000000
VERSION     DAOQIE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20530_length_3125_cov_7.493160"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PK144_17285"
     CDS             complement(<1..1634)
                     /locus_tag="PK144_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF67527.1"
                     /translation="MKIQIERAYIESLVSAFPDLAALQDQLRFGNRVEVLATRLSRPQ
                     LDHLVGLYAAAGTDMRGPLAQLTTLQQAINDDGVRFAPGELEQAVPAIARFLVQDALR
                     GWLFAASVAGKPLPYVVTRLDYTPAGNDESGKVTLELKANARASLAVVTLRLSESDTV
                     GRTVAEIFAAKGYLKETPALIAQYDASVERYFAWRSQYGAQFSGRGTGFYAEDPSASH
                     RHTDWSRKDVVVLSASGGAARLVNDEGIITQRVTALDTPGDILGHYLGKAAKSNRYDA
                     EDEVRDLHAELPAGLFTQLPVHAYLLMFHLDLHHYLWVHADDIEPYAYQPQLKDKLVL
                     PEEQTDLIDILTAEMDVLMDDIVAGKSGGTTVLCAGPAGVGKTLTAEVYAEIIGRPLY
                     RVHSGQLGLNVAAMETALKEVLTRAQRWGAVMLIDEADVYIKRREDDMTMNAVVGVFL
                     RVLEYFNGLLFLTTNRVDDIDEAIVSRCIALIRFAPPDLEARRRIWRVMTEQFSVPAD
                     AGMIDLLSELFPAATGRDIKGLTKLVAKYCSHKQTAP"
     gene            complement(1819..>3125)
                     /locus_tag="PK144_17290"
     CDS             complement(1819..>3125)
                     /locus_tag="PK144_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNF67528.1"
                     /translation="LATLGSAVGLGNIWKFPTLTGANGGAAFLCVYLLATFAVALPVM
                     IAELAIGRRARADAVTSFERLAPDGSGRRWRWVGGAGVLAAVLLLAFYSEVAGWVFAY
                     LADAVSGTALSTDAAANAARFGALSADPLRALGWQWLVLAGTALILACGVTRGIEAVT
                     RRLMPVLFLLLALLAVRALLLPGAAEGLKFLFAPDFSKLTAGVVLTALGLAFFKLSLG
                     IGAMVTYGSYFRADQDIPRTALRVMLADLSVSLLAGIAIFPAVFSFGVEPSAGPALVF
                     MTLPAVFAQLPFGGLLLALFFALMAIAATGAMLSILEVPVAWLVQRGWPRPRAALLAT
                     LAVALPGVPAALSQGVLADATLFGRTAFELYDFASSGVLMPLVAIATALFVGRAWTRT
                     QFADALGGPAGRPSRLAFALRVLLRWVAPVAIAVVLVQGWIG"
BASE COUNT          514 a         1104 c         1098 g          409 t
ORIGIN      
        1 ggcgccgtct gcttgtggct gcagtacttg gccaccagct tggtcaggcc cttgatgtcg
       61 cggccggtgg cggccgggaa gagttcggac agcaggtcga tcattccggc atccgccggc
      121 acgctgaact gttcggtcat gacccgccag atccggcgcc gggcctcaag gtccgggggt
      181 gcgaagcgga tcagcgcgat gcagcgcgag acgatggcct cgtcgatgtc atcgacgcgg
      241 ttcgtggtca ggaacagcag gccgttgaag tactccagca cccgcaggaa cacaccgacg
      301 acggcgttca tcgtcatgtc gtcctcgcgc cgcttgatgt agacatcggc ctcgtcgatc
      361 agcatcaccg cgccccagcg ctgggcgcgg gtcaggactt ccttcagtgc ggtttccatc
      421 gccgcgacgt tcaggcccag ctgcccggaa tgcacgcggt acagcggccg gccgatgatt
      481 tccgcgtaga cctcggcggt cagcgtcttg ccgacgccgg cggggcctgc gcacagcacc
      541 gtcgtgccgc cggacttgcc ggcgacgatg tcgtccatca gcacgtccat ctcggcggtc
      601 aggatgtcga tcaggtcggt ctgctcctcg ggcagcacga gcttgtcctt cagctgcggc
      661 tggtaggcat agggctcgat gtcgtcggcg tgcacccaca ggtaatggtg caggtcgagg
      721 tggaacatca gcagataggc atgcaccggc aattgcgtga acaggccggc gggcagttcg
      781 gcgtgcagat cgcggacctc gtcttcggcg tcgtagcggt tgctcttcgc cgccttgccg
      841 aggtagtggc cgaggatgtc gccgggcgtg tcgagcgcgg tgacgcgctg cgtgatgatg
      901 ccttcgtcat tgacgagccg cgccgcgccg ccgctcgccg acagcacgac cacgtccttg
      961 cgcgaccagt cggtgtggcg atggctggcg ctcgggtctt cggcgtagaa gccggtgccg
     1021 cgcccggaga actgcgcgcc gtactggctg cgccacgcga aatagcgctc gacgctggcg
     1081 tcgtactggg cgatcagcgc cggggtttcc ttgagatagc ccttggcggc gaagatttcc
     1141 gcgaccgtgc ggccgaccgt gtcgctctcg gacaggcgca gcgtcacgac cgccaggctg
     1201 gcccgggcat tggccttcag ttcgagcgtg accttgccgc tctcgtcgtt gccggccggc
     1261 gtgtaatcga ggcgggtgac gacatacggc agcggcttgc cggcgacgct ggccgcgaac
     1321 agccagccgc gcagcgcatc ctgcacgaga aagcgggcaa tggccggcac cgcctgttcc
     1381 agctcgcccg gcgcgaagcg cacgccgtca tcgttgatgg cctgctgcag cgtcgtcagc
     1441 tgcgccagcg gcccgcgcat gtccgtgccg gccgccgcgt acaacccgac cagatgatcg
     1501 agctgcggcc gcgacagccg ggtggcgagg acctcgaccc ggttgccgaa gcgcagctga
     1561 tcctgcagcg cggccagatc ggggaaggcg ctcaccagcg actcgatgta ggcacgttcg
     1621 atctggatct tcatgggggc tccgcagcag tcgggggtat tccgacggct ccagcaaagc
     1681 tcgtgccccg caccggatcg ttccgttaca aggacttggc cgtgccgcac tccgccatcg
     1741 tgtacggcgt ccgacaagcc gggcgtcgcg agccgcacat cggcgccggt gcagggccgc
     1801 gcaccccggc ggccgtgctc agccgatcca gccctgcacc agcaccaccg cgatcgccac
     1861 cggcgccacc cagcgcagca gcacgcgcag cgcgaaagcg agccgcgacg gccggccggc
     1921 ggggccgccc agcgcatcgg cgaactgcgt gcgcgtccag gcgcggccga cgaacagcgc
     1981 ggtggcgatc gcgaccagcg gcatcagcac gccggaactg gcgaagtcgt acagctcgaa
     2041 ggcggtgcgc ccgaacagcg tcgcgtcggc gagcacgccc tgcgacagcg ccgccggcac
     2101 gccgggcagc gcgacggcga gcgtcgcgag cagcgcggcg cgcggccggg gccagccgcg
     2161 ctgcaccagc cacgcgaccg gcacttcgag gatcgacagc atcgcgccgg tcgcggcgat
     2221 cgccatcagc gcaaagaaca gcgcgagcag cagcccgccg aacggcagtt gcgcgaacac
     2281 cgccggcagc gtcatgaaca ccagcgccgg cccggccgac ggctcgacgc cgaagctgaa
     2341 cacggccggg aagatggcga tgccggcgag cagcgacacc gacaggtccg ccagcatcac
     2401 gcgcagcgcc gtgcgcggga tgtcctgatc ggcgcggaag tacgagccgt aggtgaccat
     2461 cgcgccgatg ccgagcgaca gcttgaagaa cgcgagcccc agcgccgtca gcacgacgcc
     2521 ggccgtgagc ttcgagaaat ccggcgcgaa caggaacttc aggccctcgg ccgcgcccgg
     2581 cagcagcagc gcgcggacgg cgagcagcgc gagcagcagg aacagcaccg gcatcagccg
     2641 gcgcgtgacc gcctcgatgc cgcgcgtgac gccgcaggcg aggatcagcg ccgtgccggc
     2701 gagcaccagc cactgccagc cgagcgcgcg cagcggatcg gcgctcagcg cgccgaagcg
     2761 cgcggcgttg gcggcggcat cggtcgaaag cgccgtgccg ctcacggcat cggcgagata
     2821 ggcgaacacc cagccggcga cctcgctgta gaacgcgagc agcagcaccg ccgcaagcac
     2881 gccggccccg ccgacccagc gccagcgccg cccgctgccg tccggcgcca gccgctcgaa
     2941 gctcgtgacc gcatcggcgc gcgcacgccg gccgatcgcg agttccgcga tcatcaccgg
     3001 cagcgcgacc gcgaaggtcg cgagcagata cacgcacagg aaggccgcac cgccgttggc
     3061 gccggtcagc gtcggaaact tccagatgtt gccgagcccg accgcggagc cgagcgtggc
     3121 gagca
//
LOCUS       DAOQIE010000394         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_20714_length_3108_cov_7.494923, whole genome shotgun sequence.
ACCESSION   DAOQIE010000394 DAOQIE010000000
VERSION     DAOQIE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20714_length_3108_cov_7.494923"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PK144_17295"
     CDS             complement(<1..797)
                     /locus_tag="PK144_17295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNF67529.1"
                     /translation="MTPPPEAVSPLLQFGSYFDLALADPDDGGAPQQAADLIDRAQHL
                     RYEVYCREFGFEREEDCPGQRERDEYDARSLHCLLMHHASHHDAGTVRLVLSDPGRPQ
                     APMPFVAHCGASLFTDHPAHPSRLAPELCCEVSRLAVASTFRKRFGENESPIGQIDGI
                     DISAPELRTYPLISLALFVAATRLVAACGCLHAYAIMEPRLARRLAAAGLKFERIGND
                     VEYHGRRAAFYIHLDTALAATAALPGMSAMYEYALARLDPQIPPARRR"
     gene            919..2184
                     /locus_tag="PK144_17300"
     CDS             919..2184
                     /locus_tag="PK144_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67530.1"
                     /translation="MHADTRHPAPELAACSGVLTLELNTGPAGSVPPGPVSRASATQI
                     AEGIAADLGRLVPDLARFALVVPGALYDQTELLRPGFPLLAALDDVFRGTLLDGGFVP
                     GLVALGSDDGRAFTLPALNPARQPGAGPLLMLPFSLLGRPGDITELSMQLENSLLQSG
                     GVSVTTRQAVEAAFGLRAVNLSYATVGDLCALLKVQLEHAGFDGLWELLERALFRPQE
                     PADVSLAPGNRFLMSEGTVWTPFWTFDDWAQIGPGQERPADALAAGYVEWVRTQRQYL
                     MALSAWGLPVRMAIGEAFTECAGTTMRDLRAGAALDGDYLVETVLHVDNAGPERRLLV
                     TNQFDDEVGTLAYTVAALDADGCLLALEHHYPLSPRGLEAIAARLTERSRDFERIVRH
                     PGGLLIDAAGRCLESAPDPATETAAGTRH"
     gene            2213..>3108
                     /locus_tag="PK144_17305"
     CDS             2213..>3108
                     /locus_tag="PK144_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNF67531.1"
                     /translation="MKLFYCDHFVLPLPPGHRFPMDKYRRLRERVVSGRDPRFELHPP
                     PAASDAELARVHDPDYIRRASRGELDARELRALGFPWSEALIERSRRSSGATIAACRA
                     ALAEGAAANLAGGTHHAAPAAAQGFCVFNDAAVAARAMQAEGRVQRVLIVDADVHQGN
                     GTALCLAGDDSVFTFSIHGAKNFPFRKETSDLDVELADGTGDDEYLAALEAGLAQAMA
                     RAAGAQLVIYLAGADPYAGDRLGRLALSKAGLAARDDLVYRRCAAAGLPVAVAMAGGY
                     ADDIGDIVDIHYATLERAAAHAG"
BASE COUNT          428 a         1156 c         1089 g          435 t
ORIGIN      
        1 cgccgccgcg ccggcggaat ctgcggatcg agccgcgcca gcgcgtattc gtacatcgcg
       61 ctcatgcccg gcagcgctgc ggtcgccgcc agcgcggtgt cgaggtggat gtagaaggcc
      121 gcgcgccggc cgtggtactc gacatcattg ccgatgcgct cgaacttcag cccggccgcc
      181 gccagccggc gcgccaggcg cggctccatg atcgcgtagg cgtgcaggca gccgcaggcc
      241 gccaccagcc tcgtggccgc gacgaacagc gccagcgaga tcagcgggta ggtgcgcagc
      301 tccggcgccg agatgtcgat gccgtcgatc tggccgatcg ggctttcgtt ttcaccgaag
      361 cgcttgcgga aggtcgaggc gaccgccagc cgcgacacct cgcagcagag ctccggggcc
      421 agccgcgacg gatgcgccgg gtgatcggtg aacaggctgg cgccgcagtg cgcgacgaag
      481 ggcatcggcg cctgcggccg gcccgggtcg gacagcacca gacgcacggt gccggcgtcg
      541 tggtggctgg cgtggtgcat cagcaggcag tgcagcgagc gggcgtcgta ttcgtcgcgc
      601 tcgcgctggc ccgggcagtc ctcctcgcgc tcgaaaccga attcgcggca gtagacctcg
      661 tagcgcaggt gctgggcacg atcgatcagg tccgcggcct gttgcggcgc gccgccatcg
      721 tcgggatcgg cgagcgcaag atcgaagtaa ctgccgaact gcagcagcgg ggaaaccgct
      781 tccggaggag gcgtcatcag caaactccat caaaaaccac aggaatcagg tgaaatcctg
      841 tcacggaccg gccgcgacgg cttccggtat agtagccgcc ccgcaaagac cggaccgccc
      901 gacgctggag ctttcgcctt gcacgccgat acccgccacc ccgcccccga actcgccgcc
      961 tgctcgggcg tgctgacgct ggagctgaac accggcccgg ccggcagcgt gccgcccggc
     1021 ccggtcagcc gcgccagtgc cacgcagatc gccgaaggca tcgccgccga cctcggccgg
     1081 ctggtgcccg atctggcgcg tttcgcgctg gtcgtgcccg gcgcgctgta cgaccagacc
     1141 gaactgctgc gcccgggctt tccgctgctc gccgcgctcg atgacgtgtt ccgcggcacg
     1201 ctgctcgatg gcggcttcgt gcccggtctg gtcgcactcg gcagcgacga cggccgcgcc
     1261 ttcacgctgc cggcgctcaa tcccgcccgc cagcccggcg ccggaccgct gctgatgctg
     1321 ccgttctcgc tgctcggccg ccccggggac atcacggaac tgtcgatgca gctcgaaaac
     1381 tcgctgctgc agtccggcgg cgtgtcggtg acgacgcgcc aggcggtcga agcggccttc
     1441 ggcctgcgcg ccgtgaatct ttcctatgcg acggtcggcg acctgtgcgc gctgctgaag
     1501 gtgcagctcg aacacgccgg cttcgacggc ctgtgggagc tgctcgaacg cgcgctgttc
     1561 cggccgcagg agccggccga cgtgagcctc gcgcccggca accgcttcct gatgagcgag
     1621 ggcacggtct ggacgccgtt ctggaccttc gacgactggg cgcagatcgg tcccggtcag
     1681 gaacgcccgg ccgatgcgct cgccgccggc tacgtcgaat gggtgcgcac gcagcgccag
     1741 tacctgatgg cgctgtccgc ctggggcctg ccggtgcgca tggcgatcgg cgaagccttc
     1801 accgaatgcg ccggcacgac gatgcgcgac ctgcgcgccg gcgccgcgct cgacggggat
     1861 tatctggtcg agaccgtgct gcacgtcgac aacgccggcc cggagcgccg gctgctggtc
     1921 accaaccagt tcgacgacga ggtcggcacg ctcgcctaca cggtcgccgc gctcgatgcc
     1981 gacggctgcc tgctcgcgct cgaacaccac tacccgctgt cgccgcgcgg gctggaggcg
     2041 atcgccgcgc gcctgaccga gcgcagccgc gacttcgagc gcatcgtccg ccaccccggc
     2101 ggcctgctga tcgacgccgc cggccgctgc ctggaatcgg ccccggaccc ggccaccgaa
     2161 accgccgccg gcacgcgcca ctgagcctgc cgatcccgcc gccggcgccg ccgtgaagct
     2221 cttttactgc gaccacttcg tgctgccgct gccgcccggc catcgcttcc cgatggacaa
     2281 gtaccggcgg ctgcgcgagc gcgtcgtcag cggccgggat ccgcgcttcg agctgcaccc
     2341 gccgccggcg gcgagcgatg ccgaactcgc ccgcgtccac gatccggact acatccgccg
     2401 cgccagccgc ggcgagctcg acgcgcgcga gttgcgcgcg ctcggcttcc cgtggtcgga
     2461 ggcgctgatc gagcgttcgc gccgctcctc gggcgcgacc atcgccgcct gccgcgccgc
     2521 gctcgccgag ggcgctgctg ccaacctcgc cggcggcacg caccacgccg ccccggccgc
     2581 ggcgcagggc ttctgcgtgt tcaacgacgc ggcggtcgcc gcgcgcgcga tgcaggccga
     2641 gggccgggtg cagcgcgtgc tgatcgtcga tgccgacgtg catcagggca acggcacggc
     2701 gctgtgtctg gccggcgacg acagcgtgtt cacgttctcg atccacggcg cgaagaactt
     2761 cccgttccgc aaggaaacga gtgatctcga tgtcgagctg gcggacggca ccggcgatga
     2821 cgagtatctg gccgcgctcg aagccgggct cgcgcaggcg atggcccgcg ccgccggggc
     2881 gcagctggtg atctatctcg ccggggccga tccgtacgcc ggcgaccggc tcggacgcct
     2941 cgcgctcagc aaggccggcc tcgcggcgcg tgacgatctg gtctaccgcc gctgcgccgc
     3001 cgccggtctg ccggtggcgg tggcgatggc cgggggctat gccgacgaca tcggggacat
     3061 cgtcgacatc cactacgcga cgctcgaacg cgccgccgcg cacgccgg
//
LOCUS       DAOQIE010000395         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_20726_length_3107_cov_6.006881, whole genome shotgun sequence.
ACCESSION   DAOQIE010000395 DAOQIE010000000
VERSION     DAOQIE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20726_length_3107_cov_6.006881"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1562)
                     /locus_tag="PK144_17310"
     CDS             complement(69..1562)
                     /locus_tag="PK144_17310"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF67532.1"
                     /translation="MADPGLTQAASTPAAVVAAVETPLAPLSELPARLGTPAIVCAHG
                     ELHARLAVLPQLRAALIRGERPPPGWPHGVLREALFDTLDRLDLPAFCTGQPELADRV
                     LLSLLFHADRTIDEPAGTSEEDAAARAAERFAADWAEMVEEVREWRWVFDALGDVAKA
                     MRWNEARGLLKSAGWQDLVRIRRLLERMEPLSVAIRRLGRALLTDDPDPDSAPVLPQY
                     ESAVEHRRQWREITVPEVAGETCGLERSARVARMLPSEAQLLGHPRLRKLWFARFAER
                     TLLTYRERNRAREHVVVEVPVLKPLPQPLPERRMERGPIVVCVDTSGSMQGAAGEVAK
                     AAVLEAMRVAHAQQRPCYVYAFSGPEDIAERELPLSVDGVEALLDFIAQSFSGGTDIA
                     GPLTRALDRMDDAQWRLADVLIASDGEFGLPRLLVERLAQAKRASGLRIQGVLIGDRE
                     TPAMRELCDDLLWVRDWRSYGPAAVSRGAAPVEARSLTAKYFPGALK"
     gene            complement(1555..2934)
                     /locus_tag="PK144_17315"
     CDS             complement(1555..2934)
                     /locus_tag="PK144_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF67533.1"
                     /translation="MSSELYERLRGLLDALSAHLIERDTEIRLSLLAALAGEHLLLLG
                     PPGTAKSELARRLRHALRDARYFERLLTRFSVPEELFGPLSIVALEQDRYERQTAGFL
                     PEASIAFIDEVFKANSAILNALLTLLNEREFDNGSRRLRTPLVTVLAASNEVPEDEAI
                     AAFHDRFLLRCDVQPVGAGRFPELLALPDDIAITVPDACRFDHAELDALRQAAAAVAL
                     PAAVVELIADLRAWTLEQGIAVTDRRWRKLAQLLRIAAHAHGRSQVDLWDCALLVYGV
                     GETAAQRATVAQWYGERLGTDRVLQPARLTRVVESFEAQLEFERTATDLNYDAAGRLA
                     LEAGAEDDDNGLRSSRRKGLSGFIRPKQYGERHIGARVEQVGALLAEFDAALGGLDRI
                     ALELEETVRLHLWLPPDFADRVREPLAATRAGFAALRARVIDVREGFAALPLADEPGT
                     APAPIAVDG"
BASE COUNT          453 a         1106 c         1127 g          421 t
ORIGIN      
        1 tgccgcagcc gctggcagcc gggtggtggg cgtggcggcg ctggcagcgg ctgccgcgct
       61 gaagcgcgct acttcagcgc gcccgggaag tacttcgccg tcaggctgcg cgcctcgacc
      121 ggcgccgcgc cgcggctgac ggcggcaggg ccgtagctgc gccagtcgcg cacccacagc
      181 aggtcgtcgc agagctcacg catcgccggc gtctcgcgat cgccgatcag cacgccctgg
      241 atgcgcaggc cgctcgcgcg cttcgcctgc gcgaggcgct cgaccagcag ccgcggcagg
      301 ccgaattcgc cgtcgctggc gatcagcaca tcggcgagcc gccactgcgc atcgtccatg
      361 cgatcgagcg cgcgcgtgag cgggccggcg atgtcggtgc cgcccgagaa gctctgtgcg
      421 atgaaatcga gcagcgcctc gacgccgtcg acgctcagcg gcagctcgcg ctcggcgatg
      481 tcctcggggc cggagaacgc atagacgtag cagggccgct gctgcgcgtg cgcgacgcgc
      541 atcgcttcga gcaccgcggc cttggcgacc tcgccggccg cgccctgcat cgaacccgag
      601 gtatcgacgc agacgacgat cggcccgcgc tccatgcgcc gctcgggcag cggctgcggc
      661 aggggcttga gcaccggcac ctcgacgacg acgtgctcgc gcgcgcggtt gcgttcgcgg
      721 taggtgagca gcgtgcgttc ggcgaagcgc gcgaaccaga gcttgcgcag ccgcggatgg
      781 ccgagcagct gcgcttcgct cggcagcatg cgcgcgacgc gcgccgatcg ttcgaggccg
      841 caggtttcgc cggcaacctc gggcacggtg atctcgcgcc actggcgccg gtgttcgacg
      901 gccgattcgt actgcggcag caccggggcg ctgtcggggt cggggtcgtc ggtcagcagc
      961 gcacggccga gcctgcggat cgccaccgac agcggctcca tgcgttcgag cagccggcgg
     1021 atgcgcacca ggtcctgcca gccggccgat ttcagcagac cgcgcgcctc gttccagcgc
     1081 atcgccttgg cgacatcgcc gagcgcgtcg aacacccagc gccactcgcg cacttcctcg
     1141 accatctccg cccagtcggc cgcgaagcgt tcagcggccc gcgcagcagc atcctcttcg
     1201 ctggtgccgg ccggttcgtc gatggtgcgg tcggcgtgaa acagcagcga cagcagcacg
     1261 cgatcggcga gttccggctg gccggtgcag aaggccggca gatcgagccg atcgagcgtg
     1321 tcgaacagcg cctcgcgcag cacgccgtgc ggccagccgg gcggcggccg ttcgccacgg
     1381 atcagcgccg cccgcagttg cggcagcacg gcgaggcgcg cgtgcagctc gccgtgtgcg
     1441 cagacgatgg ccggcgtgcc gaggcgcgcc ggcagctcgg acaagggtgc aagcggggtc
     1501 tcgaccgcgg cgacgacggc ggccggagtc gatgcggcct gcgtcagccc gggatcagcc
     1561 atcgacggcg atcggcgccg gagccgtgcc gggctcatcg gcgagcggca gcgcggcgaa
     1621 gccttcgcgc acgtcgatca cgcgcgcgcg cagcgccgca aacccggcac gggtcgcggc
     1681 gagcggctcg cggacgcgat cggcgaaatc cggcggcagc cacagatgca gccgcacggt
     1741 ctcttccagt tcgagcgcga tgcgatccag cccgccgagc gcggcatcga attcggcgag
     1801 cagcgccccg acctgctcga cgcgcgcgcc gatgtgacgc tcgccgtact gcttgggacg
     1861 gatgaagccg gagaggccct tgcgccgcga gctgcgcagg ccgttgtcgt cgtcctcggc
     1921 gccggcttcg agcgcgaggc gcccggcggc gtcgtagttc aggtcggtcg ccgtgcgctc
     1981 gaattcgagc tgcgcctcga aggactcaac gacgcgcgtc aggcgcgccg gctgcagcac
     2041 gcgatcggtg ccgaggcgct cgccgtacca ctgcgcgacc gtggcgcgct gcgcggcggt
     2101 ttcaccgacg ccgtacacga gcagcgcaca gtcccacaga tcgacctggc tgcgcccgtg
     2161 cgcgtgcgcg gcgatgcgca gcaactgcgc gagcttgcgc cagcgccggt cggtgacggc
     2221 gatgccctgc tccagcgtcc aggcgcgcag gtcggcgatc agctcgacga cggcggccgg
     2281 cagcgccacg gcggccgcgg cctggcgcag cgcatcgagc tcggcgtgat cgaagcggca
     2341 ggcatccggc accgtgatcg cgatgtcatc cggcagcgcc agcagttccg ggaagcgtcc
     2401 ggcaccgacc ggctgcacgt cgcagcgcag caggaagcgg tcatggaagg ccgcgatcgc
     2461 ctcatcctcg ggcacctcgt tgctcgcggc gagcacggtc accagcggcg tgcgcaggcg
     2521 ccggctgccg ttgtcgaact cgcgctcgtt cagcagcgtc agcagcgcgt tcaggatcgc
     2581 actgttggcc ttgaacacct cgtcgatgaa cgcgatcgag gcctcgggca ggaagccggc
     2641 ggtctggcgc tcgtagcggt cctgttcgag cgcgacgatc gacagcggcc cgaacagttc
     2701 ctcgggcacc gagaagcgcg tcagcagacg ctcgaagtag cgcgcatcgc gcagcgcatg
     2761 ccgcagccgg cgcgcgagtt cgctcttggc ggtgccgggc ggcccgagca gcagcaggtg
     2821 ctcgccggcg agcgccgcca gcagcgacag ccggatctcg gtatcgcgct cgatcagatg
     2881 ggccgacagg gcatcgagca gcccgcgcag ccgttcgtac agctcactgc tcatgcgagc
     2941 gacctccgga agcggaatcc tggcagtcta ctgccaccgg ccctgccatg aggggtccgg
     3001 aagcggcaga ccgctgccgt gttgccttca ttccggagaa accattacgg gacgggatga
     3061 acttcgtttc ccggcaatgg cttgatgctg atcgtttccg gagtttc
//
LOCUS       DAOQIE010000396         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21074_length_3076_cov_6.108904, whole genome shotgun sequence.
ACCESSION   DAOQIE010000396 DAOQIE010000000
VERSION     DAOQIE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21074_length_3076_cov_6.108904"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PK144_17320"
     CDS             <1..1087
                     /locus_tag="PK144_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67534.1"
                     /translation="VGSGRYDTAWFERLRARPETGFVVPRTRQIAATAELRPLPVATA
                     AEPQSAALPSAAGAPAAAAAAGSRPLTVELIPSAAGDALLARWQLAAPEGTQLVLSQP
                     AAEKLGLAPGARVEAVIVRTRAGREEGARVTLTLAGIAPLAAFDRDGAFVALDLLEAA
                     EDWRDGFGVPRLEWPGEARPGKGRVYPGFRLYARTLEDVAPLRSALVAEGLEVVTQAD
                     AIDNVRRLARNLDLLFAAIAALGGSGYVAATAAASLATVERKRRELSLLRLLGLPTRG
                     LLLFPLAQAACTAGFGLLLAFVLYFGASFGLNLLFATDTGEQLCRLLPQHAAIAVGAT
                     LTAVALAALVGGLRATRIEPSEGWREV"
     gene            complement(1146..1559)
                     /locus_tag="PK144_17325"
     CDS             complement(1146..1559)
                     /locus_tag="PK144_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNF67535.1"
                     /translation="MSALRVVLDTSVLVSGFACPGSVPGRIGAAWRQGGLEVVLSRYI
                     LDELVRVLPRLPRVHLTQTEIRDLADSFMFIADVVEPEGVQDATLRDPADQPVLLTLL
                     ASKANYLITGDKDLLALASQYPVVRPADFWARHGI"
     gene            complement(1559..1831)
                     /locus_tag="PK144_17330"
     CDS             complement(1559..1831)
                     /locus_tag="PK144_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNF67536.1"
                     /translation="MITEVSAVNFRQNLGEMLNQVQYRNDSILINKDGKPVAALVDAE
                     LFARIRRMRERFDALSERIAEAYAAVPADEGMAEIDAAVAAERKQR"
     gene            complement(2526..>3076)
                     /locus_tag="PK144_17335"
     CDS             complement(2526..>3076)
                     /locus_tag="PK144_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67537.1"
                     /translation="PRAAAVLAWSMEHVRNRPEKEAVMPEFNRISAELSDQDLTELRA
                     LVATARSKLPFLRGLTTQERKELAKLGDKTVGFDEKVQAYMVTNPEFVPGFIDTAEVH
                     KDRLLRTRFMGFLSEFMTLAEHMDDTMTILGSEILMADLAYYQSVREAAKRGVPGAQQ
                     IYDDLRTRFPGGGGRTAASATA"
BASE COUNT          439 a         1024 c         1071 g          542 t
ORIGIN      
        1 ggtcggcagc ggccgctacg acacggcctg gttcgagcgc ctgcgcgcgc gccccgagac
       61 cggcttcgtc gtgccgcgca cgcgccagat cgccgcgacc gccgaactgc gcccgctgcc
      121 ggtggcgacc gccgccgagc cgcaatccgc cgcgctgccc agcgccgccg gggcgcccgc
      181 ggccgctgcc gcggcaggca gccggccgct gaccgtcgag ctgatcccga gtgctgccgg
      241 cgatgcgctg ctcgcgcgct ggcagctcgc cgcccccgag ggcacgcagc tcgtgctgtc
      301 gcagccggcg gccgaaaagc tcgggctcgc gcccggtgcc cgggtcgagg ccgtgatcgt
      361 gcgcacgcgc gccgggcgcg aggagggcgc gcgcgtgacg ctgacgctcg ccggcatcgc
      421 cccgctcgcg gccttcgatc gcgacggcgc cttcgtcgcg ctcgatctgc tcgaagccgc
      481 cgaggactgg cgcgacggtt tcggcgtgcc gcggctggaa tggcccggcg aggcgcgacc
      541 cggcaagggg cgggtgtatc cgggtttccg gctgtacgcg cgcacgctgg aggatgtggc
      601 accgctgcgc agtgcgctgg tggccgaggg gctggaggtg gtcacgcagg ccgacgccat
      661 cgacaacgtc cgccgcctcg cccgcaacct cgacctgctg ttcgccgcca tcgccgcgct
      721 cggtggcagc ggctacgtgg cggcgaccgc cgcggcgagc ctcgccaccg tcgagcgcaa
      781 gcgccgcgaa ctgtcgctgc tgcgcctgct cggcctgccg acccgcggcc tgctgctgtt
      841 cccgctcgcg caggctgcct gcaccgccgg cttcggcctg ctgctcgcct tcgtgctgta
      901 tttcggcgcg agcttcggcc tgaacctgct gtttgcgacc gacaccggcg aacagctctg
      961 ccgcctgctg ccgcagcacg cggccatcgc cgtcggcgca acgctgacgg cggtggcgct
     1021 ggccgccctg gtcgggggcc tgcgcgcgac gcgcatcgag ccgtcggagg ggtggcggga
     1081 ggtgtgaggc cgatcacggc ggaacacacc gtcttttcag cccatccccc aggtggggaa
     1141 gggaatcaga tcccgtgacg tgcccagaag tccgcaggtc ttacgaccgg atactggctc
     1201 gccagcgcca gcaagtcctt gtcgccggtg atcaggtagt tggccttcga cgccagcagg
     1261 gtgagcagca cgggttgatc ggccgggtcg cgcagcgtcg catcctgcac gccctccggt
     1321 tcgaccacat cggcgatgaa catgaagctg tctgccagat cacggatttc ggtctgcgtc
     1381 aggtgaaccc gcggcagacg gggcagcacc cggaccagtt cgtccaggat gtaccgggac
     1441 agcacgacct ccaggccgcc ttgacgccac gcagccccaa tgcgtcccgg tacgctgccc
     1501 ggacaggcaa atcccgacac cagtacgctg gtgtcgagca cgacgcgtaa agccgacatc
     1561 agcgctgctt gcgctcggcc gccacggcag cgtcgatctc ggccatgcct tcatcggccg
     1621 gcaccgcggc atacgcctcg gcgatccgtt cgctgagcgc atcgaagcgc tcccgcatgc
     1681 gccggatgcg ggcgaacagt tcggcatcga cgagtgcggc aacgggcttc ccatccttgt
     1741 tgatgaggat gctgtcgttg cggtactgca cctgattgag catttcgccc aggttctgcc
     1801 ggaagttcac ggcgctgact tcggtgatca tcatgccccc atggccatat tggtcgatat
     1861 ggtcatcatc atacggattc attgctttat ccagccggga ggcggtgggt tgtcccgtct
     1921 ggtgatgacg gccgggttgc ctgcgtcgcc ccggcctgcg cgctgaatcc tgccctgacc
     1981 gacaccggcg tgcctgcgcc ggccctgtcc aaagctccgt tttcgactca ttttggggcg
     2041 acgccgcccc gaaatgagtc ggcgtcgacg gttgaaccgt cggcgccgat ggttcaagtg
     2101 tcgtctccgg tactcggaac ccccgaacag gccatccgga tgccggtggt gctggctgaa
     2161 gtgccgtttc cggtgttccg gataccggcg ccggggagca aagcctctgc gttgtcactt
     2221 caactatcga cgccgactca ttttagggcg gcggcgaccc aaaataagtc ggcgtggttt
     2281 gttcaggagt cggcctggat tgttgaagta tcggcgtcga ctgttcagga gtcggcgtgg
     2341 attgttgaag agtcggcttg gactgttgaa gtgtcggcgc ggactgttca ggagtcggcg
     2401 tcgattgctg aagtgtcggc atcgagtgtt caggagccgg cgtcgacgat tcagcagagg
     2461 gcgccggccg ttcaggcgtc gacaccgacc gttcaggagt cggtgccggc gctcgggcag
     2521 ggggctcagg ccgtggcgga tgcggcggtg cggccgccgc cgccggggaa gcgggtacgc
     2581 aggtcgtcgt aaatctgctg cgcgcccggc acgccgcgtt tggcggcttc gcgcacgctc
     2641 tggtaatagg ccagatcggc catcaggatt tcgctgccga gaatggtcat cgtgtcgtcc
     2701 atgtgctcgg cgagcgtcat gaactccgac aggaaaccca tgaagcgcgt gcgcagcagg
     2761 cggtccttgt ggacttcggc agtgtcgatg aagcccggca cgaattccgg gttggtgacc
     2821 atgtaggcct gaaccttctc gtcgaaaccg acggtcttgt cgccgagttt cgcgagttcc
     2881 ttgcgctcct gcgtcgtcag accgcgcagg aacggcagtt tgctgcgggc cgtggccacg
     2941 agtgcacgca gctcggtaag gtcctggtcg ctcagttcag cggaaatgcg gttgaattcg
     3001 ggcatgacgg cttccttttc gggacggttg cggacgtgtt ccatactcca tgcaaggacg
     3061 gccgcagcgc ggggtg
//
LOCUS       DAOQIE010000397         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21320_length_3052_cov_6.404404, whole genome shotgun sequence.
ACCESSION   DAOQIE010000397 DAOQIE010000000
VERSION     DAOQIE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21320_length_3052_cov_6.404404"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PK144_17340"
     CDS             <1..1268
                     /locus_tag="PK144_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNF67538.1"
                     /translation="AGRPGTVTIATNMAGRGTDIVLGGNLSAELDALGPEADEAAREA
                     VRADWQQRHDAVVAAGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLEDSL
                     MRIFASERVSGLMQKLGMKEGEAIEHPWVTRAIENAQRKVEAHNFDIRKQLLEYDDVA
                     NEQRKVVYAQRNELLAAEDIAEAVRSMRHTVLGRVFAGFVPPGSLDEMWDLPGLEAAL
                     ETDFGLTLALRSWLEAEPNLIEENLRERVLAAADSAYDDKEALVRAEFLRRFEKDVLL
                     QVLDNHWKEHLAAMDYLRQGIHLRGFAQKNPKQEYKREAFEMFEAMLERIQHDVISFL
                     LRVQFQQEAPPLPEHHAPADAELHFEHAEVSALAEGEDEEAEATARAAAADRAAHTPF
                     VREDRKVGRNDPCPCGSGKKYKHCHGQLA"
     gene            1372..2601
                     /gene="argJ"
                     /locus_tag="PK144_17345"
     CDS             1372..2601
                     /gene="argJ"
                     /locus_tag="PK144_17345"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNF67539.1"
                     /translation="MPVNLNPPAPGSLKAIAGVTLGVTAAHVRKPDRKDVMVMTLPDG
                     ARVAGVFTQNRFCAAPVQLCRAHLAAGAPIRALVVNTGIANAGTGDDGLARAQAVCAE
                     LAGRLGCAPAQILPFSTGVIMEPLPVERIAAGLPACLADARASGWEDAAAAIMTTDTV
                     AKGSSRTLELDGRPVSVTGIAKGAGMIHPDMATMLGFVATDAAVAQPLLDALLRRVAD
                     DSFNRVTVDGDTSTNDSFVLIAAGTAGIAEIASEADPRLAVLEAAIREVAVELAQALA
                     RDGEGATKFVTIEVDGGRDRRECVRIGKAIAHSPLVKTALFASDPNLGRLLAAIGYAG
                     IDDLDVSALRLWLGPVLVAEHGGRAAGYVEAEAAAAMAEAEITIRVELGRGSARDRVW
                     TCDFSYDYVKINAEYRS"
     gene            2598..>3052
                     /gene="mutT"
                     /locus_tag="PK144_17350"
     CDS             2598..>3052
                     /gene="mutT"
                     /locus_tag="PK144_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNF67540.1"
                     /translation="MSGDASGSGRGCVHVAAGALVDRAGRVLIAQRPARAHQGGLWEF
                     PGGKLEAGESVQAALARELHEELGIVVRRARPLIAVEHAYADKTVRLDVWRIEDWSGV
                     PHGREGQPLAWCDPQALDPVAFPAADVPVLAALRLPPLYLVTGEPAQEP"
BASE COUNT          460 a         1114 c         1071 g          407 t
ORIGIN      
        1 aggccggccg gcccggcacc gtcacgatcg ccaccaacat ggccggccgc ggcactgaca
       61 tcgtgctcgg cggcaatctt tcggccgaac tcgacgcgct cggccccgag gccgacgagg
      121 cggcccgcga ggcggtgcgt gccgactggc agcagcgcca cgacgccgtg gtcgccgccg
      181 gcggcctgca catcgtcggc accgagcgcc acgagtcgcg gcgcatcgac aaccagctgc
      241 gcggccgctc cggccgtcag ggcgatccgg gttcctcgcg cttctacctg tcgctcgaag
      301 acagcctgat gcgcatcttc gcgtccgagc gcgtctcggg cctgatgcag aagctcggca
      361 tgaaggaagg cgaggcgatc gagcatccgt gggtcacgcg cgcgatcgag aacgcccagc
      421 gcaaggtcga ggcgcacaac ttcgacatcc gcaaacagct gctcgaatac gacgacgtgg
      481 ccaacgagca gcgcaaggtc gtctacgcgc agcgcaacga gctgctcgcc gccgaggaca
      541 tcgccgaggc cgtgcgctcg atgcgtcaca cggtgctcgg ccgcgtgttc gccggcttcg
      601 tgccgccggg cagcctcgac gagatgtggg atctgcccgg cctcgaagcc gcgctcgaaa
      661 ccgacttcgg cctgacgctg gcgctgcgca gctggctgga ggccgagccg aacctgatcg
      721 aggaaaacct gcgcgagcgc gtgctggcgg cggccgacag cgcctatgac gacaaggaag
      781 cgctggtgcg cgccgagttc ctgcgccgct tcgagaagga cgtgctgctg caggtgctcg
      841 acaaccactg gaaggagcat ctggcggcga tggattacct gcgtcagggc atccacctgc
      901 gcggtttcgc gcagaagaac ccgaagcagg aatacaagcg cgaagccttc gagatgttcg
      961 aggccatgct cgaacgcatc cagcacgacg tgatcagctt cctgctgcgc gtgcagttcc
     1021 agcaggaagc cccgccgctg cccgagcacc acgcgccggc cgatgccgag ctgcacttcg
     1081 agcacgccga ggtctcggcg ctcgccgaag gcgaggacga ggaagccgag gccacggccc
     1141 gtgccgccgc cgccgatcgc gccgcgcaca cgcccttcgt gcgcgaggac cgcaaggtcg
     1201 gacgcaacga cccctgcccc tgcggttccg gcaagaagta caagcactgc cacggccagc
     1261 tcgcctgagc ctccgccgcc gtggccggcg ggcacgaccg cgcgtcgtgc ccgccgggat
     1321 ccgatatccc gttccgatgc cctttccgat gccgatccgc ggagaaccgc catgccggtg
     1381 aacctgaacc cgcccgcgcc gggcagcctg aaagccatcg ccggcgtcac gctcggcgtc
     1441 accgccgcgc acgtgcgcaa gccggaccgc aaggacgtga tggtcatgac gctgccggac
     1501 ggtgcgcgcg tcgccggcgt gttcacgcag aaccgcttct gcgccgcccc ggtgcagctc
     1561 tgccgcgcgc atctggccgc cggcgcgccg atccgtgcgc tggtcgtcaa caccggcatc
     1621 gccaatgccg gcaccggcga cgacggcctc gcgcgcgcgc aggccgtctg tgcggaactg
     1681 gccgggcggc tcggctgcgc accggcgcag atcctgccgt tctcgaccgg cgtgatcatg
     1741 gagccgctgc cggtcgagcg catcgccgcc ggcctgccgg cctgtctcgc cgatgcccgc
     1801 gccagcggct gggaggacgc cgccgctgcg atcatgacca ccgacacggt ggccaagggc
     1861 agctcgcgca cgctcgaact cgacggccgc ccggtgagcg tcaccggcat cgccaagggc
     1921 gccggcatga tccatccgga catggcgacg atgctcggct tcgtggccac cgacgcggcg
     1981 gtggcgcagc cgctgctcga tgcgctgctc aggcgcgtcg ccgatgactc cttcaaccgc
     2041 gtcaccgtcg acggcgacac ctcgaccaac gattccttcg tgctgatcgc cgccggcacg
     2101 gccggcatcg ccgagatcgc gagcgaggcc gacccgcgcc tcgccgtgct cgaagccgcg
     2161 atccgcgagg tggcggtcga gctggcgcag gcgctggcgc gcgacggcga aggcgcgacc
     2221 aagttcgtga ccatcgaggt cgatggcggg cgggaccgcc gcgaatgcgt gcgcatcggc
     2281 aaggccatcg cccattcgcc gctggtgaag acggcgctgt tcgcgtccga cccgaacctc
     2341 ggccgcctgc tcgcggcgat cggctatgcc ggcatcgacg atctcgacgt cagcgcgctg
     2401 cgcctgtggc tcggaccggt gctggtcgcc gagcacggcg ggcgtgccgc cggctacgtc
     2461 gaggccgagg ccgctgcggc gatggccgag gccgagatca cgatccgcgt cgagctcggt
     2521 cgcggcagcg cccgcgatcg cgtctggacc tgcgacttct cgtacgacta cgtgaagatc
     2581 aacgccgagt accgttcttg agcggagatg ccagcggctc cggccgcgga tgcgtgcacg
     2641 tcgccgccgg cgcgctggtc gatcgcgccg ggcgcgtgct gatcgcgcag cgcccggcgc
     2701 gcgcgcatca gggcgggctg tgggagtttc cgggcggcaa gctcgaagcc ggtgaaagcg
     2761 tgcaggccgc gctcgcgcgc gagctgcacg aggaactcgg catcgtcgtg cgtcgcgccc
     2821 ggccgctgat cgcggtcgag cacgcctacg ccgacaagac cgtgcgcctc gatgtctggc
     2881 gcatcgagga ctggagcggc gtgccgcacg gccgtgaagg ccagccgctc gcctggtgcg
     2941 atccgcaggc gctcgacccg gtcgcgtttc cggccgccga cgtgccggtg ctcgcggcgc
     3001 tgcgcctgcc gccgctatac ctcgtgaccg gcgagccggc gcaggaaccg ga
//
LOCUS       DAOQIE010000398         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21404_length_3046_cov_8.655634, whole genome shotgun sequence.
ACCESSION   DAOQIE010000398 DAOQIE010000000
VERSION     DAOQIE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21404_length_3046_cov_8.655634"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="PK144_17355"
     CDS             complement(<1..1661)
                     /locus_tag="PK144_17355"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HNF67541.1"
                     /translation="MNDTQHAFDRAALARVWRLTRAYWTSEEKLSAWALLIIVLTGNF
                     TIVYLTVRLNSWYRGFYDALQNYDAPAFWAALGTFGILASVFILVSGTRYLLRMWLRI
                     RWRTWLTKRYQHAWLHDKAFYRMKLVGSAVDNPDQRIADDLNSFTEQTMTLVFGLVNS
                     VTTLVSFSVILWGLSSTLPLTIGDTQFHIPGFLFWGALVYAVLGTWLTHRIGHALIPL
                     NFQQERVEADYRFNLMRLRENLESVALYGGEQVEDGRLRTRFRRVIDNYHRLIIAGFK
                     VDMFNAGYSQVSVIVPYLTVAPFYFATKMPFGQLMQIGNAFNRVQDAMSFFIDSYQNI
                     AAWRAVTLRLTQFTEALESLEARIDDISQVERSSAPRPQTETLGIRRPDGEALLDGLD
                     LDLQPGESLLISGASGSGKSTLLRTLAGLWPYAKGRVRLPGDGVLFVPQKPYLPIGTL
                     REALLYPQQQTVSDDALQAVLAEVGLERLAGQLETVEDWQQTLSGGEQQRVAFARILL
                     RRPRWLFLDEASSALDEPSEQALYALLRARLPETTIVSIGHRSTLA"
     gene            1932..>3046
                     /locus_tag="PK144_17360"
     CDS             1932..>3046
                     /locus_tag="PK144_17360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67542.1"
                     /translation="MRILTFSTLYPNATAPQFGVFVENRLRQLLASGEVEARVVAPVP
                     WVPGDYGWLGSYARLARIPAEEHRHGIAVRHPRYPQLPRIGETLHPLGLALGALPALR
                     RLQREGFDFELIDAHYFYPDGVAAALLARWLGKPFVVTARGTDLVTIAKHPLPQRMIR
                     AAAEAADGLITVSASLAGELAGLGIASERVTVLRNGVDLAHFRPAEREAARARFGVSG
                     PAPVLASVGHLIPRKGNEWILRALPALPGVRLLIAGQGPEQAMLEGLIRELGLAGRVT
                     LLGEVAHAELSQLYSAADVLVLATGHEGWPNVLLEAMACGTPVLANAVSGCPEIVTAP
                     EAGRLMPDRQPATIATHAAALLAALPDRAATRRYAEG"
BASE COUNT          450 a         1055 c         1090 g          451 t
ORIGIN      
        1 gcgagcgtgc tgcggtggcc gatgctgacg atcgtcgttt ccggcagccg tgcgcgcagc
       61 agcgcataca gcgcctgttc ggagggttcg tcgagtgcgg aactcgcctc gtcgaggaac
      121 agccagcgcg gccggcgcag caggatgcgt gcgaaggcga cgcgctgctg ctcgccgccc
      181 gagagcgtct gctgccagtc ctcgaccgtt tcgagctggc cggcgagccg ctccagaccg
      241 acctcggcga gcacggcctg caaggcgtca tcgctcaccg tctgctgctg cgggtacagc
      301 agcgcctcgc gcagcgtgcc gatcggcagg tagggcttct gcggcacgaa cagcacgccg
      361 tcaccgggca ggcgcacgcg gcccttcgcg tagggccaca gtccggccag cgtgcgcagc
      421 agcgtgctct tgccgctgcc cgaggcaccg ctgatcagca gcgactcgcc gggctgcagg
      481 tcgagatcga gcccgtcgag cagcgcctcg ccgtccgggc ggcggatgcc gagcgtctcg
      541 gtctgcgggc gcggcgcgct gctgcgctcg acctgcgaga tgtcgtcgat gcgcgcctcc
      601 agcgattcca gcgcctcggt gaactgcgtc aggcgcagcg tcacggcgcg ccacgcggcg
      661 atgttctggt agctgtcgat gaagaagctc atcgcgtcct gcacgcggtt gaaggcgttg
      721 ccgatctgca tcagctggcc gaacggcatc ttcgtcgcga agtagaacgg cgcgacggtc
      781 aggtagggca cgatcaccga cacctggctg tagccggcgt tgaacatgtc gaccttgaag
      841 ccggcgatga tcagccggtg gtaattgtcg atgacgcggc gaaagcgcgt gcgcagccgc
      901 ccgtcctcga cctgctcgcc gccgtacagc gccaccgatt cgaggttctc gcgcagccgc
      961 atcaggttga agcggtagtc ggcctcgacg cgctcctgct ggaagttcag cgggatcagc
     1021 gcatggccga tgcggtgcgt gagccaggtg ccgagcaccg cgtacaccag cgccccccag
     1081 aacaggaagc cggggatgtg gaactgcgtg tcgccgatgg tcagcggcag cgtcgaggac
     1141 aggccccaga ggatcaccga gaagctcacc agcgtcgtga ccgagttcac gagcccgaac
     1201 accagcgtca tcgtctgctc ggtgaagctg ttgagatcat cggcgatgcg ctggtcgggg
     1261 ttgtcgacgg cgctgccgac cagcttcatg cggtagaagg ccttgtcgtg cagccaagcg
     1321 tgctggtagc gcttggtcag ccaggtgcgc cagcggatgc gcagccacat gcgcagcagg
     1381 tagcgcgtgc cggagacgag gatgaaaacg ctcgcgagga tgccgaaggt gccgagcgcg
     1441 gcccagaacg ccggcgcgtc gtagttctgc agcgcgtcgt agaagccgcg ataccaggaa
     1501 ttcagccgca ccgtcaggta gacgatcgtg aagttgccgg tcagcacgat gatcagcagc
     1561 gcccaggcgc tgagcttttc ctcggaggtc cagtaggcgc gcgtcagtcg ccagacgcgg
     1621 gcgagggcgg cgcggtcgaa cgcatgctgc gtgtcgttca tcggcaggag gctccggaag
     1681 gctgcacgcg caggaggcag gcggcaggcg aagggaagga aggacaggga cgggcgcgcg
     1741 ctcggaagga acacggcctt gtaatcgcgc cgcggccccg gcgttttagc ggacgcttaa
     1801 ccgcctcgcg ccgggcgctg cggcgtcgga tcggtgcggg ccgcccgcgg ttcccgtcgc
     1861 ggcgggcgcg gatgatagcc gtgcaccggc ccgtatactc cgcgccctta cgtcacggcc
     1921 ggtgtccgcg gatgcgcatc ctcacgttct cgacgctcta ccccaacgcc acggcgccgc
     1981 agttcggcgt gttcgtcgag aaccgcctgc gccagctgct cgcgagcggc gaggtcgagg
     2041 cgcgcgtcgt cgcgccggtg ccgtgggtgc cgggtgacta cggctggctc gggtcgtatg
     2101 cgcggctcgc gcgcatcccg gccgaagaac accgtcacgg catcgcggtg cgtcacccgc
     2161 gctatccgca gctgccgcgc atcggcgaaa ccctgcatcc gctcgggctc gcgctcggcg
     2221 cgctgccggc gctgcgccgg ctgcagcgcg agggcttcga cttcgagctg atcgacgcgc
     2281 actacttcta tcccgacggc gtcgccgcgg cgctgctggc gcgctggctc ggcaagccct
     2341 tcgtggtcac ggcgcgcggc accgatctgg tcaccattgc gaagcacccg ctgccgcagc
     2401 gcatgatccg cgccgccgcc gaagccgccg acggcctgat cacggtctcg gcctcgctcg
     2461 ccggagaact cgccggactc ggcatcgcca gcgagcgcgt gacggtgctc aggaacggcg
     2521 tcgatcttgc gcacttccgg ccggccgaac gcgaggccgc gcgcgcgcgc ttcggcgtca
     2581 gcggcccggc gccggtgctg gcctcggtcg ggcacctgat cccgcgcaag ggcaacgaat
     2641 ggatcctgcg cgcgctgccg gccctgcccg gcgtgcgcct gctgatcgcc gggcagggac
     2701 cggagcaggc catgctggaa gggctgatcc gcgagcttgg cctcgccggc cgggtcacgc
     2761 tgctcggcga ggtggcgcac gccgagctgt cgcagctcta ttccgccgcc gacgtgctgg
     2821 tgctcgcgac cggccacgaa ggctggccga acgtgctgct cgaagccatg gcctgcggca
     2881 cgccggtgct cgcgaacgcc gtctccggct gcccggagat cgtcaccgct ccggaagccg
     2941 gccggctgat gccggaccgc cagcccgcca ccatcgccac gcacgccgct gcgctgctcg
     3001 ccgcactgcc ggatcgcgcg gcgacgcgcc gctacgccga aggctt
//
LOCUS       DAOQIE010000399         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21423_length_3044_cov_7.340917, whole genome shotgun sequence.
ACCESSION   DAOQIE010000399 DAOQIE010000000
VERSION     DAOQIE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21423_length_3044_cov_7.340917"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PK144_17365"
     CDS             complement(<1..386)
                     /locus_tag="PK144_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNF67543.1"
                     /translation="MQSFTYPVALTADEHDGGFVVTCRDIPEAITQCESPADCLEEAE
                     GALQAAVEARIMDDLDIPPASEPVHGEIAVSLPVTTALKAALYLSMRDAGLSKSALAR
                     RLGIDEKEARRMLTPGHPTKAVVLERA"
     gene            complement(397..801)
                     /locus_tag="PK144_17370"
     CDS             complement(397..801)
                     /locus_tag="PK144_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNF67544.1"
                     /translation="MPAAQGCAHAAQAFAHARHLHLPALHASIDACSAGIDACATAVA
                     VRSKMTDAAVHVMATPEARAMKGSEFIHKVRRYAKASGLAFEWRPELGKGSHGVLILG
                     GRRTVVRNLKDELKTGTLHAMLRQLGIAADDL"
     gene            complement(864..2834)
                     /locus_tag="PK144_17375"
     CDS             complement(864..2834)
                     /locus_tag="PK144_17375"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF67545.1"
                     /translation="MRIPRLRPSLALLILLFALLPALAAPVAEELTLFHVGDQESWLI
                     SAQGNRYDTPDDPISFYGGAARLSALLQQQRTAALAAGRAVLVLNAGDSFLPGPRLTA
                     SLKHLAQAAPGGGQDFYDAIVLRRLGFTAVCFGNHEFDLGPDVTAQFVAVAGTPYLSA
                     NLDFSGHAALQALVAEGRIAKTLIVDTPKGNRIAVIGATTPRLPDISSPGAVTVRDRD
                     PAADEDTKLLALAPLLQAEIDRARSAGASVVVLLSHLQGYRNELKLVPKLSGLDVFVS
                     GGGHELMTSPGMATILDEPAMIRGYPQVVALEDGSRVLGVTGNFGNRYVGELQLSLDA
                     QGRVRRDADGVPLTGTGSRMHRVSGNPADADHVLPDPVIEAGVVQPVRAFIAALSEQK
                     IATTPVELNGVRGSAAESGAQAQYGVRNSETNLGNLVADAVRFAAGTDLAIQNGGGIR
                     TSIRAGDVSAGDVFNVLPFTNLIVQFRGLDAGQLRILMEHALANASPDGAASGRFAQV
                     SGLRIVYDSRRPPLQLDADGRPAGGGRILSMTLDDGTQLVEDGIVRTGARPLSLATID
                     FTARGGDGYPFARLGLTPEPLVHTIHYQEALLDYLSQPADKGGLGGVIDTRRYGPVDP
                     QAGGGRLIDRALLKPVSGLPGRVSVGRREAAV"
BASE COUNT          461 a         1077 c         1045 g          461 t
ORIGIN      
        1 gcgcgctcca gaacgactgc cttcgtcggg tgacccgggg tcagcatgcg ccgcgcttcc
       61 ttctcgtcga tgccgagccg tcgggcgagc gccgacttgc tgagcccggc atcgcgcatc
      121 gagaggtaca gcgcggcttt gagcgccgtc gtgaccggca gggataccgc aatctctccg
      181 tgcacaggct cgctcgctgg cgggatgtcc agatcgtcca tgatgcgagc ctcgacggcg
      241 gcctgcaggg cgccttcggc ttcttccagg caatccgccg ggctttcaca ctgcgtgatc
      301 gcttcgggga tgtcccggca ggtcacgacg aacccgccat cgtgctcgtc ggccgtgagt
      361 gctacggggt aggtgaagct ctgcatgatg gcctccttac agatcgtccg ccgcgatgcc
      421 cagttgcctg agcatggcgt gcaaagtgcc cgtcttgagt tcgtccttga ggttgcgcac
      481 gaccgtgcgc cgtccgccga ggatcagcac gccgtggctg cccttgccaa gctcgggccg
      541 ccactcgaac gcgagcccac tggcttttgc gtaccgccgg accttgtgga tgaactcgct
      601 gcccttcatg gccctggctt ccggcgttgc cattacatgc actgcagcgt cggtcatttt
      661 tgaccgaaca gcaaccgctg tggcacatgc atcgatccct gcgctgcatg catcgatcga
      721 tgcatgcagc gccggcaagt gcagatgccg cgcatgggcg aatgcctgcg cggcatgggc
      781 gcacccttgc gccgcaggca tcgacccatc cacggcaagt gcggaccctt cggccggcag
      841 ggccgatgct cactcgtcag ggatcaaacc gccgcctcgc gcctgccgac gctcacccgc
      901 cccggcagcc ccgacaccgg cttcagcagc gcccgatcga tcagcctgcc cccgcccgcc
      961 tgcggatcga ccggcccgta gcgcctcgtg tcgatcacgc cgccgagccc gcccttgtcc
     1021 gccggctgac tcaggtagtc gagcagcgcc tcctgataat ggatcgtgtg caccagcggc
     1081 tccggcgtca gcccgaggcg tgcgaacgga tagccgtcgc cgccacgggc cgtgaagtcg
     1141 atcgtcgcga gcgacagcgg ccgggcaccg gtgcggacga tgccgtcctc gaccagctgc
     1201 gtgccgtcat cgagcgtcat cgacaggatg cgtccgccgc ccgcgggcct gccgtcggca
     1261 tcgagctgca gcggcgggcg gcgcgagtcg tagacgatgc gcaggcccga aacctgcgcg
     1321 aagcgcccgc tcgcggcccc gtccggcgag gcattcgcca gcgcatgctc catcaggatg
     1381 cggagctgcc cggcgtccag cccgcggaac tgcacgatca ggttcgtgaa cggcagcacg
     1441 ttgaacacgt cgccggcgct cacgtcgcca gcccggatcg aggtgcggat gccgccgccg
     1501 ttctggatcg cgaggtcggt gccggcggcg aagcgcacgg cgtcggcgac caggttgccg
     1561 aggttggtct cgctgttgcg cacgccgtac tgcgcctgcg cgccggactc cgccgccgag
     1621 ccgcgcacgc cgttgagttc gacgggggtc gtggcgatct tctgctcgct cagcgccgcg
     1681 atgaacgccc gcaccggctg cacgaccccg gcctcgatga ccggatcggg caggacgtga
     1741 tcggcatcgg ccgggttgcc gctgacgcgg tgcatgcggc tgccggtgcc ggtcagcggc
     1801 acgccgtcgg catcgcgccg cacccgcccc tgcgcatcga gcgacagctg cagttcgccg
     1861 acgtagcgat tgccgaagtt gccggtgacg ccgagcacgc ggctgccatc ctcgagcgcg
     1921 acgacttgcg gatagccccg gatcatggcc ggctcatcga ggatcgtcgc catgccgggc
     1981 gaagtcatca gctcgtggcc gccgccggag acgaacacgt cgaggccgct gagcttcggc
     2041 accagcttca gctcgttgcg gtagccctgc aggtggctga gcagcacgac gacgctcgcg
     2101 cccgccgagc gcgcgcggtc gatctcggcc tgcagcaagg gcgcgagtgc cagcagcttc
     2161 gtgtcctcgt ccgcggccgg gtcacggtca cgcaccgtga ccgccccggg cgaggagatg
     2221 tccggcagcc gcggcgtggt cgcaccgatc acggcgatcc ggttgccctt cggcgtatcg
     2281 acgatcaggg tcttcgcgat ccgtccttct gcaaccagcg cctgcagcgc ggcatggccg
     2341 gagaaatcga ggttggccga cagatacggc gtgccggcca ccgcgacgaa ctgcgcggtc
     2401 acgtccggcc cgaggtcgaa ctcgtgattg ccgaagcaga cggccgtgaa gccgagccgg
     2461 cgcagcacga tggcatcgta gaaatcctgc ccgccccccg gggcggcctg cgcgagatgc
     2521 ttcaggctcg ccgtcagccg cggccccggc aggaaggaat ccccggcatt gagcaccagc
     2581 accgcccgcc ctgccgccag cgctgccgtg cgctgctgct gcagcagcgc cgaaagccgc
     2641 gcggccccgc cgtagaagct gatcggatca tccggcgtgt cgtaccggtt gccctgcgcg
     2701 ctgatcagcc aggattcctg atcaccgacg tggaacagcg tcagttcctc ggccaccggc
     2761 gcggcgagtg ccggcagcag tgcaaacagc aggatcaaaa gggcgaggct ggggcgaagc
     2821 cggggaatcc gcatgattcg cgctccggga aggatcgggc aggccgcggg tggcgtggcg
     2881 ccgggacgca cgacgggggc gaaaaggttc agggcacgga tgcagggccg gctccgtgcg
     2941 catcgcacgg atcgaccgga gcggagggca aagtccggca gccgccagcg aaggatgaca
     3001 cggcgcttcg ctccggatca tgccctccgg gcacggcggc ggtt
//
LOCUS       DAOQIE010000400         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21667_length_3023_cov_8.203167, whole genome shotgun sequence.
ACCESSION   DAOQIE010000400 DAOQIE010000000
VERSION     DAOQIE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21667_length_3023_cov_8.203167"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1118)
                     /locus_tag="PK144_17380"
     CDS             complement(402..1118)
                     /locus_tag="PK144_17380"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF67546.1"
                     /translation="MKRLRIAVFGYHKLGIEVAKHLAPTPCELLVIDSSDDNLARAAE
                     RGHATLKCDYTDDLELARAGIGRDIDLIFCLLPDEAQNVFLIISARALDERLTIVAVS
                     DSPGSEQKLRAAGADKVIDPFDIAGRRISDLLVRPTVVDVLDQTVFGAADLEIAEVAV
                     TREGRLAGRRLLDASRLAGGTLVIVGLVRSGLERELRFALEAPEYVLTPGDVLMVVGP
                     AADVLAFRAEAAAGDAQRAI"
     gene            complement(1115..2731)
                     /locus_tag="PK144_17385"
     CDS             complement(1115..2731)
                     /locus_tag="PK144_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF67547.1"
                     /translation="MTAAPAHHLVRCCYALAASPGYRRTKRFAYDLLENPASRLRPYF
                     DMAMCALILATVGLIIHEVKNPPTPFTLVFEDTVMYVFIAEYLARMWVWNDSHRLVIE
                     AWERAELLGLPFRLRPVLAALLRQKWAYATQPMAIIDLLAILPSYRPLRTLRFFLLFR
                     VFKLFRYTRSINEFARVLAEKRFEFYTLATFIGFVVFASATAIYILEAHVETSHVNTL
                     YDAVYWAVITIFTVGYGDIVPKTPEGRLVAIVLVVIGVAVISFSTSIVVNAFSEKMQT
                     MRDRRLYADTEKLHDYVIVCGYGRVGEVVVDRLVATGTPFVIIDRHAKNILRAKAQAR
                     LAICDDAVRAELLGQVGITRGAKSILCVTGDDVTNLYITLTARQMNPHIRIVSRANKS
                     ETVRKLRLAGADHVVMPYEIVGLMAAEYIHQPVASMALSGILSGERGMAMDVVLVAAH
                     SLLDGRRIADIGFAARRLTLFGVLAAPGAVQRPALIRHPLADGRDFYFNPPAAFTLQA
                     GDQLIVLGRELSIQHLRAQVEASALARRPA"
     gene            complement(2928..>3023)
                     /locus_tag="PK144_17390"
     CDS             complement(2928..>3023)
                     /locus_tag="PK144_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNF67548.1"
                     /translation="HELAAEYGVSAERIRQIEAGALKKLKTALAA"
BASE COUNT          470 a          992 c         1125 g          436 t
ORIGIN      
        1 ctcgacgaca tggccggcgc tttcgagcag caggcgcagg gccgtgcgca gctcgcggtc
       61 atcatcgacg atgccgacgc ccggaggcgg cggatgcgtg gtctcggtgt tcatgtgcct
      121 gtcggcgcgc atcgcctccg ttgcgctgcg ccgccccggt gctgctggaa cggggggtcc
      181 gatgttctcc cgtcatgcca ggtcagtcgg atgaatccgg agcacggcgt ctgcatgcca
      241 tatccggcct gccccgcgac gcctgtgccc gaggatcagg aatatcggat tcggaagcat
      301 tgcaccagat caattctgcg gagggttttc gcacggctgc gcgccggatc cggtcgtcgc
      361 cgggcgggcg gggccgtgcc ggaggcccgg cgcgcagccg gctagatcgc gcgctgggca
      421 tccccggctg cggcctcggc gcggaaggcg agcacgtcgg cggccgggcc gaccaccatc
      481 agcacgtcgc cgggcgtcag cacgtactcg ggcgcttcga gcgcgaagcg cagctcgcgc
      541 tccagacccg agcgcacgag gccgacgatg accagcgtgc cgccggcgag gcggctcgca
      601 tccagcaggc gccgcccggc cagccggccc tcgcgggtca cggcgacctc ggcgatctcc
      661 agatcggcgg cgccgaacac ggtctggtcg agcacgtcga cgacggtcgg acgcaccagc
      721 aggtcggaga tgcggcggcc ggcgatgtcg aacgggtcga tgaccttgtc ggcgccggcg
      781 gcacgcagct tctgctccga tcccggcgaa tcggacacgg cgacgatggt caggcgctca
      841 tcgagcgcgc gcgccgagat gatcaggaac acgttctgcg cctcgtcggg cagcaggcag
      901 aagatcaggt cgatgtcgcg gccgatgccg gcgcgcgcga gttcgaggtc atcggtgtaa
      961 tcgcacttga gcgtcgcgtg gccgcgctcg gcggcgcgcg cgaggttgtc gtccgagctg
     1021 tcgatgacca gcagctcgca cggcgtcggc gcgaggtgct tggcgacctc gatgccgagc
     1081 ttgtggtagc cgaacacggc gatgcgcagg cgcttcatgc cggcctccgg gcgagggcgc
     1141 tggcttcgac ctgggcgcgc aggtgctgga tcgacagttc gcgcccgagc acgatgagct
     1201 ggtcgccggc ctgcagcgtg aaggccgcgg gcgggttgaa gtagaaatcc cgcccgtcgg
     1261 cgagcgggtg gcggatcagc gccgggcgct gcacggcccc gggcgcggcg agcacgccga
     1321 acagcgtcag cctgcgcgcg gcgaagccga tgtcggcgat gcgccggccg tcgagcagcg
     1381 aatgcgcggc gaccagcacc acatccatcg ccatgccgcg ctcgccggag aggatgccgc
     1441 tcagcgccat cgacgccacc ggctggtgga tgtactcggc cgccatcagg ccgacgatct
     1501 cgtagggcat gacgacgtga tcggcgccgg cgagcctgag cttgcgcacg gtttcggact
     1561 tgttggcgcg cgagacgatg cggatgtgcg ggttcatctg ccgcgcggtc agcgtgatgt
     1621 agaggttggt cacgtcgtcg ccggtcacgc acaggatgct cttcgcgccg cgcgtgatgc
     1681 cgacctggcc gagcagctcg gcgcgcaccg cgtcgtcgca gatcgccagc cgggcctgcg
     1741 ccttggcgcg gaggatgttc ttggcgtggc ggtcgatgat cacgaacggc gtgccggtgg
     1801 ccaccagccg gtcgacgacg acctcgccga cccggccgta gccgcagacg atcacgtagt
     1861 cgtgcagctt ctcggtatcg gcgtacaggc gccggtcgcg catcgtctgc atcttctcgc
     1921 tgaaggcgtt gacgacgatc gaggtcgaga acgagatcac cgccacgccg atcaccacca
     1981 gcacgatcgc caccagccgg ccctcgggcg tcttcggcac gatgtcgccg tagccgaccg
     2041 tgaagatcgt gatcaccgcc cagtagacgg cgtcgtagag cgtgttgacg tggctggtct
     2101 cgacgtgggc ttcgaggatg tagatcgcgg tcgccgacgc gaacacgacg aagccgatga
     2161 aggtcgccag cgtgtagaac tcgaagcgct tctcggcgag cacgcgcgcg aactcgttga
     2221 tcgagcgcgt gtagcggaac agcttgaaga cgcggaacag caggaagaag cgcagcgtgc
     2281 gcagcggccg gtacgagggc aggatcgcca gcaggtcgat gatcgccatc ggctgcgtcg
     2341 cataggccca cttctggcgc agcagcgcag cgagcaccgg ccgcagccgg aacggcaggc
     2401 cgagcagctc ggcgcgctcc caggcctcga tcaccagccg gtgcgagtcg ttccacaccc
     2461 acatgcgcgc caggtactcg gcgatgaaca cgtacatgac cgtgtcctcg aacacgagcg
     2521 tgaacggcgt cggcgggttc ttgacctcgt ggatgatcag cccgaccgtc gcgaggatca
     2581 gcgcgcacat cgccatgtcg aagtacggcc gcagccgcga ggccgggttt tcgagcaggt
     2641 cgtaggcgaa gcgcttggtg cgccggtagc cgggactggc ggcgagcgcg tagcagcagc
     2701 ggacgaggtg atgggcgggc gcggcggtca tggtcgcgaa ggttcgggcg ggcggccgtg
     2761 ccgacggcag gagacagagg tggggcagta tggccgcagc cggccgtgcc tgcagcgatc
     2821 ccggtcaagc gggacgatcg ggtggccccc ggccgcccgc atgaaagccg acgccggggc
     2881 cgtcgcagcg gacggccccg gcgtcggggc agggaagggc gtgaggttca ggcggcgagc
     2941 gcggtcttca gcttcttcag cgcgccggcc tcgatctggc ggatgcgctc ggccgagacg
     3001 ccgtactcgg cggccagctc gtg
//
LOCUS       DAOQIE010000401         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21889_length_3005_cov_6.815932, whole genome shotgun sequence.
ACCESSION   DAOQIE010000401 DAOQIE010000000
VERSION     DAOQIE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21889_length_3005_cov_6.815932"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PK144_17395"
     CDS             <1..617
                     /locus_tag="PK144_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67549.1"
                     /translation="RLHMTSGQQQFLAALGDLALSLLPDGRRRGRQTPAALIEAASEA
                     LFGPWTWPDPEHALGWDPDGERLHALRPLAPTADSANKSVRFAVWLAHFALALLPALP
                     VRGRLQTTGFVPERRGGQRRFRWPLWESPIGPDTLAALLGTPELTAADDGGIAALHAR
                     GVVAVYESQRHEFGQGYAVLRPARLIPPAAPQVRRSAPAGPLVF"
     gene            620..2308
                     /gene="cas1"
                     /locus_tag="PK144_17400"
     CDS             620..2308
                     /gene="cas1"
                     /locus_tag="PK144_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNF67550.1"
                     /translation="MATDADPTLAAVPPLPARMVNEYVYCPRLAYLMWVQQEWTESAE
                     TVDGQYVHRQVDRPGGVLPDEPLPEGERIHARSVTLESATLGVIAKLDLVEAEGLRAT
                     PVDYKRGRRPHAARGPHAPERVQLALQGLLLREHGFECREGVIWYAGSRERVPVTLDE
                     DLVTEARSAVAGLRALATAGRLPPPLDDSPKCPRCALVGICLPDETARLRGGDLAPRP
                     LAVADDPAQLLFVQAPYARIGRDGETLTVEIEREPAKPVRLIDVSQLVLHGNVSLSAP
                     ALHELMARDIPVSWHSHGGWFLGHTIGTGHRNVELRSAQYRASFDEAACLRLARGLVE
                     AKIANARTLLRRNWKQGSAPPALLAALKDDIATARTAPSLASLLGVEGAAAARYFGVL
                     AELLNPAPAADGGYSFDFRTRNRRPPADPVNALLSFAYALLTRTLTVTLTAVGFDAYR
                     GFYHQPRYGRPALALDVMEPFRPLIADSVVLQVINNGEVRPRDFVRAGGGVALKPEAR
                     KALVLAFERRLAHEVTHPLFGYRVAYRRLLEIQARLLGRHLLGELPDYPNFTTR"
     gene            2314..2613
                     /gene="cas2"
                     /locus_tag="PK144_17405"
     CDS             2314..2613
                     /gene="cas2"
                     /locus_tag="PK144_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNF67551.1"
                     /translation="MSRFQEHLYVVVYDIRDDRRWRRVFRTMKGFGDWVQLSVFQCRL
                     TAVRHAELVARLDGLILHGHDHVLVMDMGPATGIAPRVTSLGLPYEPPEKGPVVL"
BASE COUNT          411 a         1093 c         1045 g          456 t
ORIGIN      
        1 cccgcctgca catgacctcc ggccagcagc agttcctcgc cgcgctcggc gatctggcgc
       61 tgtcgctgct gccggacggc aggcggcgcg gccggcagac gccggcggcg ctcatcgaag
      121 ccgcttccga ggcgctgttc gggccgtgga cctggcccga ccccgagcac gcgctcggct
      181 gggacccgga cggcgagcgc ctgcacgcgc tgcgtccgct ggccccgacc gccgacagcg
      241 ccaacaagag cgtgcgcttc gcggtctggc tcgcgcactt cgcgctggcg ctgctgccgg
      301 cactgccggt gcgcggccgg ctgcagacca ccggcttcgt gcccgagcgc cgcggcggcc
      361 agcgccgttt ccgctggccg ctgtgggaaa gcccgatcgg tcccgacacg ctggccgcgc
      421 tgctcggcac gccggagctg accgccgccg atgacggcgg catcgccgcg ctgcacgcgc
      481 gcggcgtggt cgcggtgtac gagtcgcagc gccacgagtt cggtcagggc tacgccgtgc
      541 tgcgcccggc ccggctgatc ccgccggccg ctccgcaggt ccgtcgcagc gcaccggccg
      601 gtccgctggt gttctgaaca tggcgaccga cgccgacccc acgctcgccg ccgtgccgcc
      661 gctgccggcg cgcatggtca acgagtacgt gtactgcccg cggctcgcgt acctgatgtg
      721 ggtgcagcag gaatggaccg agagcgccga aaccgtcgac ggccagtacg tgcaccggca
      781 ggtcgatcgc cccggcggcg tgctgcccga tgagcccctg cccgaaggcg agcgcatcca
      841 tgcgcgctcg gtgacgctcg aatccgccac gctcggcgtc atcgcgaagc tcgatctggt
      901 cgaggccgaa ggtctgcgcg ccacaccggt cgactacaag cgcggcaggc gcccgcacgc
      961 ggcgcgcggc ccgcatgcac ccgagcgcgt gcagctcgcg ctgcagggcc tgctgctgcg
     1021 cgagcacggc ttcgagtgcc gggaaggcgt gatctggtac gccggcagcc gcgagcgcgt
     1081 gccggtgacg ctcgatgagg acctcgtcac cgaagcgcgc agcgcggttg ccggcctgcg
     1141 tgcgctggcc acggccggcc ggctgccgcc gccgctcgac gacagcccga agtgcccgcg
     1201 ctgcgcgctg gtcggcatct gcctgcccga cgaaaccgcg cgactgcgcg gcggcgatct
     1261 ggcgccgcga ccgctggcgg tggccgatga cccggcgcag ctgctgttcg tgcaggcgcc
     1321 gtatgcgcgc atcggccgcg acggcgagac gctgaccgtc gagatcgagc gcgagccggc
     1381 aaagccggtg cgcctgatcg atgtctcgca gctcgtgctg cacggcaacg tcagcctcag
     1441 cgccccggcg ctgcacgagc tgatggcgcg cgacattccg gtgagctggc acagccacgg
     1501 cggctggttc ctcggccaca ccatcggcac cgggcaccgc aacgtcgaac tgcgcagcgc
     1561 ccagtaccgc gcgagcttcg acgaggccgc ctgcctgcgg ctggcgcgcg gtctggtcga
     1621 ggccaagatt gccaatgccc gcacgctgct gcggcgcaac tggaaacagg gcagcgcgcc
     1681 gccggcgctg ctcgcggcgc tgaaggacga catcgccacc gcccgcacgg caccgtcgct
     1741 cgcgagcctg ctcggcgtcg aaggcgctgc ggcagcacgc tatttcggcg tccttgccga
     1801 gctgctgaat ccggcgccgg cggccgatgg cggatacagc ttcgacttcc gcacccgcaa
     1861 ccgccgcccg ccggccgatc cggtcaacgc gctgctgtcg ttcgcctacg cgctgctgac
     1921 acgcacgctg acggtgacgc tgacggcggt cggtttcgat gcctatcgcg gcttttatca
     1981 ccagccgcgc tacggccgcc cggcgctggc gctcgacgtg atggagccgt tccggccgct
     2041 gatcgccgat tcggtggtgc tgcaggtgat caacaacggc gaggtgcgcc cgcgcgattt
     2101 cgtgcgcgcc ggcggcggcg tggcgctgaa gccggaggcg cgcaaggcgc tggtgctggc
     2161 cttcgagcgg cggctggcgc acgaggtcac gcatccgctg ttcggctatc gcgtcgccta
     2221 ccgccggctg ctggaaatcc aggcacggct gctcggccgg catctgctcg gcgaactgcc
     2281 ggattacccg aacttcacga cgcggtgagc gcgatgagcc ggttccagga gcatctgtat
     2341 gtggtcgtct acgacatccg cgatgaccgg cgctggcggc gggtcttccg gaccatgaag
     2401 ggcttcggcg actgggtgca gttgtcggtg ttccagtgcc ggctgacggc ggtgcgccac
     2461 gccgagctgg tggcgcggct ggacgggctg atcctgcatg gccatgatca cgtcctggtc
     2521 atggacatgg gcccggccac cggcatcgcc ccgcgcgtca ccagcctcgg cctgccctac
     2581 gaacctccgg aaaaaggccc ggtcgtgctg taactgccgc agtgctcgcg agcgctccgg
     2641 tgccacgaaa atcccgggca gcgctcgcaa accggaagct ctttgtcaat cagcaactta
     2701 gaatggcgct tgccgccatg tccgcacgta agtcaacatc cctcccgccc catcccgaca
     2761 gcgctcgcaa accccatcgc aagcacttgt ccggaaaggg attttcgagt ggtccgtttt
     2821 ccgcggtgta atgccgcggc ctcattgaag cccttgattc ccgctgcgaa gtcggcggcc
     2881 gacaccctgg ttttccgcgg tgtaatgccg cggcctcatt gaagccgtgg tgatcgcgat
     2941 catggccgca gcgtgttggc tggcgttttc cgcggtgtaa tgccgcggcc tcattgaagc
     3001 cagcc
//
LOCUS       DAOQIE010000402         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_21979_length_2997_cov_8.389191, whole genome shotgun sequence.
ACCESSION   DAOQIE010000402 DAOQIE010000000
VERSION     DAOQIE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21979_length_2997_cov_8.389191"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..693
                     /locus_tag="PK144_17410"
     CDS             88..693
                     /locus_tag="PK144_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNF67552.1"
                     /translation="MAAIPKFDEPVCEEPAFRLTSIGVEDYLAAEDGAGFRSEYIAGQ
                     VFAMTGASATHNIICVNLALALAPHARAGGCQFFLNDMKLRLSLRGIDCFYYPDAMIC
                     CDPADDHPLYRERPCLLVEVLSESTARIDRREKYLSYTQIAGLRGYLLLDQDAARAQL
                     HRCDDDGLWQQQSFDDPAAVLDLPCAGAVPLAELYAGLHIG"
     gene            761..2806
                     /gene="prlC"
                     /locus_tag="PK144_17415"
     CDS             761..2806
                     /gene="prlC"
                     /locus_tag="PK144_17415"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HNF67553.1"
                     /translation="MNAENPLLDFSGLPRFAEIRPEHVEPAVDALLAENRAGLAQLLA
                     QPGPYTWANLIEPLEAMQDRLNRAWGPVGHLNAVMNSEALRAAYNACLPKLSAYYTEY
                     NQNEALYRATQAIRDGDEFARLDAAQRKVIDDSLRDFRLAGIALPAEKKARYGEIVQE
                     LSLLGAKFSEHVLDATQAWSLHLDSENRLAGLPASALGLARQAAAARGLDGWLLTLEF
                     PSYQPVMSYAQDRSLRESLYFAYATRAAGNFPAGAQWDNTAVVGRILELRHELARLLD
                     FPDYAQYSLATKMADDPATVLDFLRDLGARARPQAGREFEELSAFARERLGLEKLEAW
                     DVGYASEQLKQARYAISDEELRPYFPETSVVPGLFQLVEKLFGVSIRAAEGVEVWHPD
                     VRAYDIFAADGALRARFYFDLYARANKRGGAWMDSAIARRRTADGLQLPVAYMVCNFT
                     PPVGDDPALFTHAEVQTLFHEFGHGLHHMLTTVDYAAVSGISGVEWDAVELPSQFMEN
                     FCWERVTLDLIAGHYRTREPIPEALYERMIAAKNFQSAMITVRQLEFGLFDLRLHREY
                     DPARGARVLELLEEVRDEVAVVRPPAFNRYPMSFSHIFAGGYAAGYYSYKWAEVLSAD
                     AFGAFEEAGVFDAETGCRFLTEILEVGGSRPALESFMAFRGRRPEIDALLRHSGIAA"
BASE COUNT          444 a         1066 c          993 g          494 t
ORIGIN      
        1 gcggccgcgg cccgcctgcc tgcaccggcc gggcgtggcc ggcgtagagt gtcggcacgg
       61 gcatggcgca gcagcgaggg gcggacgatg gcagcgatac cgaagttcga cgagccagtg
      121 tgcgaagaac cggcgttcag gctgacttcg atcggtgtcg aggattacct cgccgccgag
      181 gatggcgcgg gtttccgcag cgagtacatc gccggtcagg tgtttgcgat gaccggcgcc
      241 agtgccacgc acaacatcat ctgcgtgaat ctggcactcg cgctggctcc gcacgcccgg
      301 gcgggcggct gtcagttctt tctgaacgac atgaagctgc gactgtcgct gcgcggcatc
      361 gattgcttct actacccgga tgcgatgatc tgctgcgatc cggccgacga tcatccgctg
      421 tatcgcgagc gtccgtgcct gctggtcgag gtgctgtcgg agtcgacggc gcgcatcgac
      481 cggcgcgaga aatacctgtc ctatacccag atcgccggcc tgcgcggcta tctgctgctc
      541 gatcaggatg ccgcgcgtgc gcagctgcat cgttgcgacg acgacggtct ctggcagcag
      601 cagtccttcg acgaccccgc ggccgtgctc gatctgccct gcgccggtgc cgtgcctctt
      661 gccgaactct atgccggcct ccatatcggc tgatccggcc gcgccgcgcc tgcctcgttc
      721 ccggatcgtt cccgaattcc ccagcccctt caggaccgcc atgaacgccg aaaatccgct
      781 gctcgatttc tccggcctgc cgcgcttcgc cgagattcgc cccgaacacg tcgagcccgc
      841 cgtcgatgcc ctgctcgccg aaaaccgcgc cggcctcgcg cagctgctcg cgcagcccgg
      901 cccgtacacc tgggcgaacc tgatcgaacc gctggaggcg atgcaggacc ggctcaaccg
      961 tgcctggggg ccggtcgggc acctgaacgc ggtgatgaat tccgaggcgc tgcgcgccgc
     1021 ctacaacgcc tgcctgccga agctctcggc ctactacacc gaatacaacc agaacgaggc
     1081 gctgtaccgc gccacgcagg cgatccgcga cggcgacgaa ttcgcgcggc tcgatgccgc
     1141 gcagcgcaag gtcatcgacg attcgctgcg cgacttccgg ctcgccggca tcgcgctgcc
     1201 ggccgagaaa aaggcccgct acggcgagat cgtgcaggaa ctctcgctgc tcggtgcgaa
     1261 gttctccgag cacgtgctcg atgccaccca ggcctggtcg ctgcacctcg acagcgagaa
     1321 tcgcctcgcc ggcctgcccg cctcggcgct cggcctcgcg cgtcaggccg ccgcggcgcg
     1381 cggcctcgac ggctggctgc tgacgctgga gttcccgtcc taccagccgg tcatgagcta
     1441 cgcgcaggac cgctcgctgc gcgagtcgct gtacttcgcc tacgcgacgc gcgccgccgg
     1501 caacttcccg gccggcgcgc agtgggacaa caccgccgtc gtcgggcgca tcctcgaact
     1561 gcgccacgaa ctggcgcgcc tgctcgattt ccccgactac gcgcagtact cgctggccac
     1621 gaagatggcc gacgatccgg cgacggtgct cgacttcctg cgcgatctcg gcgcccgggc
     1681 ccggccgcag gccgggcgcg agttcgagga gctgtcggct ttcgcgcgcg agcgtctcgg
     1741 cctggaaaag ctcgaagcct gggatgtcgg ctacgcctcc gagcagctca agcaggcgcg
     1801 ttatgcgatc tccgacgagg agctgcgccc gtacttcccg gagacgagcg tcgtgccggg
     1861 cctgttccag ctggtcgaga agctgttcgg cgtgtcgatc cgcgctgccg aaggcgtcga
     1921 ggtctggcat ccggacgtgc gcgcctacga catcttcgcc gccgacggcg cactgcgcgc
     1981 gcgcttctac ttcgacctgt acgcgcgcgc caacaagcgc ggcggcgcct ggatggattc
     2041 ggcaatcgcc cgccggcgca cggccgacgg tctgcagctg ccggtcgcgt acatggtctg
     2101 caacttcacg ccgccggtcg gcgacgaccc ggcgctgttc acgcacgccg aggtgcagac
     2161 gctgttccac gagttcggcc acggcctgca ccacatgctg acgacggtcg actatgcggc
     2221 cgtgtccggg atttccggcg tcgaatggga tgccgtcgag ctgccctcgc agttcatgga
     2281 gaacttctgc tgggagcgcg tcacgctcga cctgatcgcc ggccattacc gcacgcgcga
     2341 gccgatcccc gaggcgctct acgaacgcat gatcgccgcg aagaacttcc agtcggcgat
     2401 gatcacggtg cgccagctcg aattcgggct gttcgacctg cgcctgcacc gcgagtacga
     2461 cccggcgcgc ggcgcccgcg tgctcgaact gctggaggag gtccgcgacg aggtcgcggt
     2521 cgtgcgtccg ccggcgttca accgctatcc gatgagcttc tcgcacatct tcgccggcgg
     2581 ctacgcggcc ggttactaca gctacaagtg ggccgaggtg ctgtcggccg acgccttcgg
     2641 cgccttcgag gaggccggcg tgttcgacgc cgagaccggc tgccgcttcc tgaccgagat
     2701 cctcgaagtc ggcggctcgc gcccggcgct ggagagcttc atggccttcc gcggccggcg
     2761 ccccgagatc gacgcactgc tgcgccacag cggcatcgcg gcctgaatcc ggtccgctgc
     2821 ccgcaaggga tgctggtcac acgggaaggc agggttttct cctccgctcc cgcgaagggc
     2881 atgggtatct aacaggtcag cggttccgcg agccggatgg atcgactgcc aacgctcggg
     2941 cttcgatctc ccctctcccc ttgcgggaga ggggccgggg gagaggggga gacgccg
//
LOCUS       DAOQIE010000403         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_22402_length_2965_cov_8.847079, whole genome shotgun sequence.
ACCESSION   DAOQIE010000403 DAOQIE010000000
VERSION     DAOQIE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22402_length_2965_cov_8.847079"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1044)
                     /gene="ppk2"
                     /locus_tag="PK144_17420"
     CDS             complement(127..1044)
                     /gene="ppk2"
                     /locus_tag="PK144_17420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNF67554.1"
                     /translation="MPIPALPDHEALLARIHEDLIDGYDEELELEIEDRTVAPGVIPD
                     RETERSERMHYFQNLFRLQGELVKLQDWIATSGQKVVIVFEGRDAAGKGGVIKRITQR
                     LNPRVCRVVALPAPNDRERTQWYFQRYVTHLPAAGEMVLFDRSWYNRAGVERVMGFCN
                     EDDVEEFFRSVPEFERMLVRSGIRVIKYWFSITDDEQHLRFMSRIHDPMKQWKLSPMD
                     LESRRRWEAYTKAKEAMLERTHIPEAPWWVVQAVDKKRARLNCISHLLELFDYAEIPR
                     PSVELPPRVRNQDYTRQPVPATMFVPERY"
     gene            complement(1239..1535)
                     /locus_tag="PK144_17425"
     CDS             complement(1239..1535)
                     /locus_tag="PK144_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67555.1"
                     /translation="MSYRTYWLTIAAGITLVLGIDYGWRTLGLHGSDPAPLAAGLFGV
                     TIGLALRRLNAGLSATALPPGRRRLRLGVQAVLALTLWAAVSLGYTLLTGAGRS"
     gene            1778..2242
                     /locus_tag="PK144_17430"
     CDS             1778..2242
                     /locus_tag="PK144_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNF67556.1"
                     /translation="MNTQTQNTQTQNTQTHSEVLAVLETWLGAIRAGDLDAIGRHYAA
                     DVSAYDAVGPLRFQGRPAYAEHWRRCLEFCEGETIYEPQPPAVFADSQLAVVHFLLRC
                     GKRKADGSEESSWMRTTLGLRREAGSHWLIFHEHSSIPFDMESGQTQFGLTP"
     gene            2352..2762
                     /locus_tag="PK144_17435"
     CDS             2352..2762
                     /locus_tag="PK144_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF67557.1"
                     /translation="MKLQPYVFFNGDAEAAAHFYRDALGAGITMLMRYRDCPETPDPA
                     HVPPGNADKVMHMSLSIGDTVLMGADDCSGSGRGFAGFSLSLSVADVAEAEKAFAALA
                     DGGSVCVPLGPTFFSPAFGMLNDRFGVSWMIVVM"
BASE COUNT          460 a          997 c         1053 g          455 t
ORIGIN      
        1 agcccctcgc ccctcgtggg agaggggttg gggagagggg gagaaaaacc atgcacttac
       61 tctcccccgt gtggtcggca tgagtcgccg gggcgggaat cccgaaccct gcgaaccctg
      121 gcggtgtcag tagcgctccg gcacgaacat cgttgccggg accggctggc gggtgtagtc
      181 ctgattgcgc acgcgcggcg gcagctcgac gctcgggcgc gggatttccg cgtagtcgaa
      241 cagttcgagc agatggctga tgcagttcag ccgcgcacgc ttcttgtcga ccgcctgcac
      301 cacccaccac ggtgcttccg ggatgtgcgt gcgctccagc atcgcctcct tggccttggt
      361 gtaggcctcc cagcgccggc gcgattcgag gtccatcggc gagagcttcc actgcttcat
      421 cgggtcgtgg atgcggctca tgaagcgcag gtgctgctcg tcgtcggtga tcgagaacca
      481 gtacttgatg acgcggatgc cggagcgcac cagcatgcgt tcgaactccg gcaccgagcg
      541 gaagaactcc tcgacatcgt cctcgttgca gaagcccatg acccgctcga cgccggcgcg
      601 gttgtaccag ctgcggtcga acaggaccat ttcaccggcc gccggcagat gcgtgacgta
      661 gcgctggaag taccactgcg tgcgctcgcg gtcgttcggc gccggcagcg cgacgacgcg
      721 gcagacgcgc gggttcagcc gctgcgtgat gcgcttgatc acgccgccct tgccggcggc
      781 atcgcggccc tcgaacacga tcacgacctt ctggccgctg gtggcgatcc agtcctgcag
      841 cttgaccagc tcgccctgca gccggaacag gttctggaag taatgcatgc gctcggaacg
      901 ttcggtctcg cgatcgggga tcacgcccgg tgccaccgtg cggtcctcga tctccagttc
      961 cagttcctcg tcgtaaccgt cgatcaggtc ttcgtggatg cgcgcgagca gcgcctcgtg
     1021 atcgggcagg gcagggatgg gcatggcagg tggtctccgg acgggcagcg tcgtgccctg
     1081 gcgcatcagt tatagccatg gtgtgtgtgc ctgcctctga cgacgcagca ctgcgcccgg
     1141 aaatccatcg ggcgcgcctg cgcagcgtcg gcgagcggtg ttgcacggcc gtgacggccg
     1201 ggccggcgtg cgcaggcgct gcggccggaa gtccgcgatc aggaacggcc ggcgccggtc
     1261 agcagcgtgt agccgaggct gacggcggcc cagagcgtga gtgccagcac ggcctgcacg
     1321 ccgagccgca gccggcgccg gccgggcggg agcgccgtcg ccgacagccc ggcgttcagg
     1381 cgccgcagcg ccaggccgat cgtcacgccg aacaggccgg cggcgagcgg ggccggatcg
     1441 ctgccgtgca ggccgagcgt gcgccagccg taatcgatgc cgagcaccag cgtgatgccg
     1501 gcagcgatcg tcagccagta ggtgcggtag gacatgggaa gcggctgcag gtcgtgggaa
     1561 gcgtcgaaag gtcgtgccgg ggccatccgt ccgcagccgg tgccgcagaa gaccctgcac
     1621 gaagtgtcgt gcatccggcg gcgggacggc gcggtttcgg tgaggtccgg gggccggcgg
     1681 acggggcgcc aggatttctg cggaaccgga gcgctgcgca ggccgtcggc atccatgccc
     1741 gtgcaagccc cgcaaccacg atccgggaga taccgagatg aacacgcaga cccagaacac
     1801 gcagacccag aacacgcaga cccattccga agtgctggcc gtgctcgaaa cctggctcgg
     1861 cgcgatccgc gccggcgacc tcgacgcgat cggccgccac tacgccgccg acgtgagcgc
     1921 ctacgatgcc gtcggtccgc tgcgcttcca gggccgcccg gcttacgccg aacactggcg
     1981 tcgctgcctg gagttctgcg agggcgagac gatctacgag ccgcagccgc cggccgtgtt
     2041 cgccgacagc cagctcgccg tcgtgcactt cctgctgcgc tgcggcaagc gcaaggccga
     2101 cggcagcgag gaaagctcgt ggatgcgcac gacgctcggc ctgcgccgcg aggccggcag
     2161 ccactggctg atcttccacg agcacagttc gattccgttc gacatggaat ccggccagac
     2221 ccagttcggg ctgacgcctt gaagcggcac ggtcacgggg ccctgccatg ctggcgggca
     2281 ccgggccggg ccagcgggtt caacgcgccg gagcggctcc gtcatgcccg gacgccagcg
     2341 aggagagaac gatgaaactg cagccctacg tgttcttcaa cggcgatgcc gaggcggccg
     2401 cccacttcta tcgcgatgcg ctcggcgccg gaatcacgat gctgatgcgc tatcgcgact
     2461 gcccggaaac gcccgatccg gctcatgtgc cgccgggcaa cgccgacaag gtcatgcaca
     2521 tgagcctgag catcggtgac accgtgctga tgggggccga tgactgcagc ggcagcggcc
     2581 ggggcttcgc cggcttctcg ctgtcgctga gcgtcgccga tgtcgccgag gccgaaaagg
     2641 ccttcgcggc gctggcggac ggcggcagcg tgtgcgtgcc gctcggcccg accttcttct
     2701 cgccggcctt cggcatgctg aacgaccgct tcggcgtgtc gtggatgatc gtcgtgatgt
     2761 gaggccgttc acaggagccc gcgtggtgtg ctgcccgcgg gcgtgcctcc ggtgccctgc
     2821 agcccgagct gcaaccggcc agaacgattc tggccgctga ccggcaggtg attggtatgg
     2881 cctgcgcatg cccatgcaaa aggccccggg atcaccgggg cctctggcag gggccgggcg
     2941 cggcgatcag cgccggcgcg cgcgc
//
LOCUS       DAOQIE010000404         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_22502_length_2957_cov_7.501378, whole genome shotgun sequence.
ACCESSION   DAOQIE010000404 DAOQIE010000000
VERSION     DAOQIE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22502_length_2957_cov_7.501378"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK144_17440"
     CDS             complement(<1..244)
                     /locus_tag="PK144_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67558.1"
                     /translation="MKIKNLLPALAASAALAASAPAAAVLIEVNIDDFNVGSHMASDN
                     TVGNGGVTSALSSLDTTHVIGGTRTLVDNLTAGIIPY"
     gene            complement(288..980)
                     /locus_tag="PK144_17445"
     CDS             complement(288..980)
                     /locus_tag="PK144_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67559.1"
                     /translation="MKHFTYALALAGLLGLNAGAQAAIVSVDVDRFEEGFQNIQDTTL
                     DSAPVTDTVSLSPANGVATSRTVAINVLSYSAGATNQLTVTADPTVSLLDVINEVGED
                     SEVTVSWVIPASLIPNDAFNTFFRIAVLQSDGNDLSVEFLLNGTALGNFAVPGNTFNQ
                     DLLFTASAAQINAGGTLEMIINGQAGWDADFDDIGFAYETPTAAPEPVSLALFGAGLI
                     GMRRLRRRAIPV"
     gene            complement(1184..1741)
                     /locus_tag="PK144_17450"
     CDS             complement(1184..1741)
                     /locus_tag="PK144_17450"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HNF67560.1"
                     /translation="MALIDVNHILPKEGYGQFTSTTCWYASYRLLHAWKQADESQIRP
                     NLEAGGLNWKELTTRGIYPEEWPVAGSRLGLCGWEGRLVKAWDDEMIVYALKGYGPLY
                     FTWDYGSSGHAVVIGGFDKKLNQFKVWNPYNRFEPGTVEIDWFTPDAFRERLHAGRRT
                     PDAGRCRPGGADPPDSSASFTGKLQ"
     gene            complement(1804..2580)
                     /locus_tag="PK144_17455"
     CDS             complement(1804..2580)
                     /locus_tag="PK144_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNF67561.1"
                     /translation="MRTRRGAGALALGLALLLALPAAQALTWRELDFDGAHYTVVELD
                     LVRDPLALYWRDPADGTPYGTLGALAEHERQRGRRLRFATNAGIYAEGQVPLGLTVEE
                     GVEQVPLNRARGGGNFFLQPNGVFAVWGRSHAAVLTSAEYARRRGPLPRLATQSGPML
                     LIDGRIHPRFQRDSDSLKLRSGVCVPAPGKVSFAISEGLVNFHAFARLFRDALGCRDA
                     LYLDGSISQMHLDGRSGLAGADRWRTRPFAALLGVSVPAD"
     gene            complement(2577..>2957)
                     /locus_tag="PK144_17460"
     CDS             complement(2577..>2957)
                     /locus_tag="PK144_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF67562.1"
                     /translation="AVYLVGSGPMIGRLGSLRFTALAMLVSTAGVLVHYLLAEGLRLP
                     QVAAPVWGLALAMALVSTVLPTFLMSAGIRRLGSSRAALVGSIGPALTIAMGAAFLGE
                     SILPLQWLGAALVVGGVLLVGSGR"
BASE COUNT          487 a         1027 c          982 g          461 t
ORIGIN      
        1 gatacggaat gatgccagcc gtcaggttgt cgacaagcgt gcgcgtaccg ccgatgacgt
       61 gcgtcgtgtc gaggctgctc agggcactgg tcacgccgcc gttgccgacc gtgttatcgg
      121 aagccatgtg gctgccgacg ttgaagtcat cgatgttgac ctcgatcagc accgccgctg
      181 ccggcgcgct cgccgccagc gccgccgaag ccgccagtgc aggaagcaga ttcttgattt
      241 tcatgcgtat cttcctgaag acccgtgccg gttgggggga atggcactca gaccgggatc
      301 gcgcggcgtc gcaggcgccg catgccgatc aggcctgctc cgaacagcgc cagcgaaacc
      361 ggttccggcg cagccgtcgg ggtttcgtag gcaaagccga tgtcgtcgaa gtcggcatcc
      421 cagccggcct gaccgttgat gatcatttcc agcgtgccgc cggcgttgat ctgcgcggca
      481 ctggccgtga acagcagatc ctgattgaag gtgttgccgg gcaccgcgaa gttgccgagc
      541 gccgtgccgt tgagcaggaa ctcgacggac aggtcgttgc cgtcggactg cagcaccgcg
      601 atccggaaga aggtgttgaa ggcgtcgttc gggatcaggc tcgccgggat cacccacgac
      661 acggtgacct cggagtcctc gccgacctcg ttgatcacat cgagcaggct cacggtcgga
      721 tcggccgtga ccgtcaactg gttggtcgca ccggcgctgt agctgagcac gttgatcgcg
      781 accgtgcgcg aggtggccac cccgttcgca gggctcagcg acacggtgtc cgtcaccggc
      841 gcactgtcga gggtcgtgtc ctggatgttc tggaagcctt cctcgaagcg gtcgacatcg
      901 accgacacga tcgccgcctg tgcgccggca ttcaggccga gcaggccggc cagcgccagc
      961 gcgtaagtga agtgcttcat gtcgcctccc gttgtccctg cgtcagggcc cgcctccagc
     1021 aacggggaag cggggttatc cacctggtgt tgcgcttcgg cccggtcttg catacccggg
     1081 ctcgactgtc aggaatcttg acgcagcgca acagccgtcg aggccgatat tcaatttccg
     1141 agccatgtcc gcaggatatt gatttgtttt aaggcaacca tgcttactgc agctttcctg
     1201 taaaggaagc tgacgagtcc ggcggatcag cgccaccagg cctgcagcgc ccagcgtccg
     1261 gcgtccggcg tccggcgtgc aggcgctcgc gaaaggcatc cggcgtgaac cagtcgatct
     1321 cgaccgtgcc gggctcgaag cggttgtacg ggttccagac cttgaactgg ttgagctttt
     1381 tgtcgaagcc gccgatgacc acggcatgcc ccgagctgcc gtagtcccac gtgaagtaca
     1441 acggcccgta gcccttgagc gcgtagacga tcatctcgtc atcccaggcc ttcaccagcc
     1501 gcccttccca gccgcagagc ccgagccggc tgccggcgac cggccactcc tcgggataga
     1561 tgccgcgcgt cgtcagctcc ttccagttca ggccgccggc ttcgaggttc gggcggatct
     1621 gcgactcgtc ggcctgcttc caggcgtgca gcaggcggta gctcgcgtac cagcaggtcg
     1681 tgctggtgaa ctggccgtag ccttccttcg gcaggatgtg attcacgtcg atcagggcca
     1741 tgacggcgct ccggagacgg ccatgcgcgg ggtgcgcacg gcatcgcctc caacgtagcg
     1801 ccctcagtcc gccggcaccg acacgccgag cagcgccgcg aacggtcgcg tgcgccagcg
     1861 atcggcaccg gcgaggccgc tgcgaccgtc gaggtgcatc tgcgagatcg agccgtcgag
     1921 gtacagcgca tcgcgacagc cgagcgcatc gcgaaacagc cgcgcgaagg cgtggaagtt
     1981 cacgagtccc tcgctgatcg cgaagctcac cttgcccggc gccggcacgc agacgccgct
     2041 cctgagcttc aggctgtccg aatcgcgctg gaagcgcgga tggatgcggc catcgatgag
     2101 cagcatcggt ccggactgcg tcgccagccg cggcagcggt ccgcgccggc gcgcgtactc
     2161 ggcgctggtc agcacggccg cgtggctgcg cccccagacc gcgaacacgc cgttcggctg
     2221 caggaagaag ttgccgccac cgcgggcgcg gttgagtggc acctgttcga cgccctcctc
     2281 gaccgtcagc ccgagcggca cctggccttc ggcgtagatg ccggcattgg tcgcgaagcg
     2341 caggcgccgg ccgcgctggc gttcgtgctc ggcgagcgcc ccgagcgtgc cgtacggcgt
     2401 gccgtcggcc ggatcgcgcc agtacagcgc cagcggatcg cggacgagat cgagctcgac
     2461 gaccgtgtag tgcgcaccgt cgaagtcgag ctcgcgccac gtgagcgcct gcgccgccgg
     2521 cagcgccagc agcagggcga gcccgagcgc gagggcgcct gcgccgcgtc gcgtcctcat
     2581 cgccccgagc ccaccagcag cacgccgccg accaccagcg ccgcgccgag ccactgcagc
     2641 ggcaggatcg attcgccgag gaaggccgcg cccatcgcga tcgtcagcgc cggcccgatc
     2701 gagccgacca gcgccgcgcg actcgatccg agccggcgga tgccggccga catcagaaag
     2761 gtcggcagca ccgtcgacac cagcgccatc gccagcgcca gtccccacac cggtgctgcc
     2821 acctgcggca gacgcagccc ttcggccagc aggtaatgca cgagcacgcc ggccgtcgac
     2881 accagcatcg ccagcgccgt gaagcgcagc gagccgagcc gcccgatcat cggcccgctg
     2941 ccgaccagat acaccgc
//
LOCUS       DAOQIE010000405         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_22631_length_2947_cov_7.327455, whole genome shotgun sequence.
ACCESSION   DAOQIE010000405 DAOQIE010000000
VERSION     DAOQIE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22631_length_2947_cov_7.327455"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK144_17465"
     CDS             complement(<1..1452)
                     /locus_tag="PK144_17465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNF67563.1"
                     /translation="MPLTDYVPDFRSPIPRGRIASTLMLLAFYLLAAGIGLRLQVYYG
                     AVTPLWPPSGLAVWMLWRRGLGLAPVILGGEWLVALWLGQGWQAQILGPLAQLVEAML
                     ASMLLRLARLPARPLDTLHGLQLFLLDAVLLAPMLAAAIGTWGLYLTGATGDGPLYET
                     LLTWWTGDALGILIITPLLLAWWPQPARSTVAQAAALMIATGIAALVVLMQAPEQRTM
                     LFFLLLPFVAIAGLLRDPAVVTLTILMLVALALGLQAIAIGHPDEFVKHVRLLFIGTV
                     AATGYIVSVLAQRLHRLANTDPLTGLPNRRRFTEQLEALAARPRGRRHALLYLDLEAF
                     KLANDTCGHAAGDRLLIEVTGRMLRRKPAAATLARMGGDEFVLLTPDHDADEASALAH
                     RLREALASKPFTVGERSFGIGMRIGIAPFGPRSESPEGVLARADLACAAARLHGGVRV
                     SGPGDGALLRHQGEIALLERLRAAFGNGRLRLAW"
     gene            complement(1732..2850)
                     /gene="ald"
                     /locus_tag="PK144_17470"
     CDS             complement(1732..2850)
                     /gene="ald"
                     /locus_tag="PK144_17470"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNF67564.1"
                     /translation="MRIGVPKEIKVHEYRVGLTPGSVRELLAHGHSVIVETGLGDGIG
                     CDDASYRCAGALVVDTAEEAWTRADLVVKVKEPQPAEIRWLREGQVVFAYLHLAPDPI
                     QCRLLMESGAAALAYETVTDAHGGLPLLAPMSEVAGRMALQAGAHCLEKTYGGLGMLP
                     GGVPGVPPAKVTILGAGTVGANAVRVAVGMGAQVAVLDRDIHRLRALDAEYGGRVTTV
                     FSTRDTIERHVLNADLVIGAVLVPGAQAPKLVGRELVARMRPGAVLVDVAIDQGGCFE
                     TSHPTTHAEPTFVHDGVIHYCVANMPGAVPRTSALALNNATLPYLLALADRGWVRALG
                     NDPGLLNGLNVLRGRVTHRAVAEALGHDYHPAGDLLAL"
BASE COUNT          440 a         1035 c         1075 g          397 t
ORIGIN      
        1 ccaggcgagc cgcagccggc cgttgccgaa cgcggcgcgc agcctttcga gcagcgcgat
       61 ctcgccctga tgacgcagca gcgcgccgtc accggggccg gagacgcgca cgccgccgtg
      121 caggcgcgcc gctgcgcagg ccagatcggc acgcgccagc acgccctccg ggctttcgct
      181 gcgcggaccg aagggggcga tgccgatgcg catgccgatg ccgaagctgc gctcgccgac
      241 cgtgaacggt ttcgaggcca gcgcctcgcg cagccggtgc gccagtgcgc tcgcctcatc
      301 cgcgtcgtga tccggggtga gcagcacgaa ctcgtccccg cccatgcgcg ccagcgtcgc
      361 cgccgccggc ttgcgccgca gcattcgccc ggtgacttcg atcagcaggc ggtcaccggc
      421 cgcgtggccg caggtgtcat tggcgagctt gaaggcttcg aggtcgagat acagcagtgc
      481 gtggcgccgg ccacgggggc gggcggcgag tgcttcgagc tgttcggtga agcggcgccg
      541 gttcggcagt ccggtcagcg gatcggtgtt cgccagccgg tgcaggcgct gcgcgagcac
      601 cgagacgatg tagccggtgg cagcgacggt gccgatgaac agcaggcgca cgtgcttgac
      661 gaattcatcg gggtgtccga tggcgatggc ctgcaggccg agggcgagcg ccaccagcat
      721 caggatcgtc agcgtcacga ccgccggatc gcgcagcagc ccggcgatcg cgacgaaggg
      781 cagcagcagg aagaacagca tcgtgcgctg ttcgggggcc tgcatcagca ccaccagcgc
      841 cgcgatgccg gtggcgatca tcagcgccgc ggcctgcgcg acggtgctgc gcgccggctg
      901 cggccaccag gcgagcagca gcggcgtgat gatcaaaatg ccgagcgcat cgccggtcca
      961 ccaggtcagg agggtctcgt acagcggccc gtcgccggtc gcaccggtca gatacagccc
     1021 ccaggtgccg atggcggctg ccagcatcgg cgccagcagc acggcatcga gcaggaacag
     1081 ctgcagcccg tgcagcgtgt cgagcggccg ggccggcagc cgcgccagcc gcagcagcat
     1141 cgaggccagc atggcttcga cgagctgcgc gagcggcccg aggatctgcg cctgccagcc
     1201 ctgaccgagc cacagcgcca ccagccactc gccgccgagg atcaccggtg ccagcccgag
     1261 cccgcgccgc cacagcatcc agacggccag cccggacggc ggccacagcg gcgtgacggc
     1321 accgtagtac acctgcaggc gcaggccgat tccggcggcg agcagataga acgccagcag
     1381 catcagcgtg ctggcgatcc ggccacgcgg gatcggtgac cggaaatcgg gcacgtaatc
     1441 ggtcagcggc atgtcggcat ccttccgcag ggcagggcaa ggcaggagcg tggagggact
     1501 gccggacgga gcgaccgctg ccgggaccgg agccgccgcc cgctgtgagg agccttattg
     1561 ccttattttt actgcatgat ttcggcttga aacaaccgtt tcattcccgg atttccgggt
     1621 gccggaagcg cttgcgcctg ccgggatttc cgggagcgga cggctgccct gccgccgtcc
     1681 atggccgtgc cgtgccggag tcgtcgccgg catgccgctg gcggcagcgg atcacagcgc
     1741 cagcagatcg ccggccgggt ggtagtcgtg gccgagcgcc tcggcgacgg cgcggtgcgt
     1801 gacgcggccg cgcaggacgt tcaggccgtt cagcagaccc gggtcgttgc cgagcgcgcg
     1861 gacccagccg cggtcggcga gcgcgagcag atacggcagc gtggcgttgt tcagcgccag
     1921 cgccgaggtg cgcggcacgg caccgggcat gttggcgacg cagtagtgga tgacgccgtc
     1981 gtgcacgaag gtcggttcgg cgtgcgtggt cgggtgcgag gtctcgaagc agccgccctg
     2041 gtcgatcgcg acatcgacca gcaccgcgcc ggggcgcatg cgcgcgacca gctcgcgccc
     2101 gaccagcttc ggcgcctgcg cccccggcac cagcaccgcg ccgatcacca gatcggcgtt
     2161 cagcacgtgg cgctcgatgg tgtcgcgggt cgagaacacc gtcgtgaccc ggccgccgta
     2221 ttcggcatcg agcgcgcgca gccggtggat gtcgcgatcg agcaccgcga cctgcgcgcc
     2281 catgccgacc gcgacgcgca cggcgttggc gccgaccgtg ccggcaccga ggatcgtcac
     2341 cttcgccggc ggcacgcccg gcacgccgcc gggcagcatg ccgaggccgc cgtaggtctt
     2401 ttcgaggcag tgcgccccgg cctgcagcgc catgcgcccg gcgacctccg acatcggcgc
     2461 gagcagcggc aggccgccgt gcgcgtcggt gacggtctcg taggcgaggg cggcggcgcc
     2521 cgattccatc agcagccggc actggatcgg gtccggcgcc agatgcaggt aggcgaacac
     2581 cacctggccc tcgcgcagcc agcggatttc ggccggctgc ggctccttga ccttcacgac
     2641 cagatcggcg cgcgtccagg cttcctcggc cgtgtcgacg acgagcgcgc cggcgcagcg
     2701 gtagctcgca tcgtcacagc cgatgccgtc accgaggccg gtctcgacga tgacgctgtg
     2761 cccgtgcgcg agcagttcgc gcacgctgcc gggcgtcagt ccgacgcggt attcatggac
     2821 cttgatctct ttcggtacgc cgatgcgcat ggcgcgactc cccgctgttc gtcccgtggt
     2881 ggttcggatg cggacgccgc tgcgtggcgg cgccttctgt attgcggcaa gcgtagggca
     2941 cgcgcag
//
LOCUS       DAOQIE010000406         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_22666_length_2944_cov_7.707165, whole genome shotgun sequence.
ACCESSION   DAOQIE010000406 DAOQIE010000000
VERSION     DAOQIE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22666_length_2944_cov_7.707165"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK144_17475"
     CDS             complement(<1..434)
                     /locus_tag="PK144_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF67565.1"
                     /translation="MKPVKLLIVDDSAVVRQVLTQVFAQSPDIRVIAAAPDPLFALEK
                     MRSEWPDVIVLDVEMPRMDGITFLRKLMSERPTPVIICSTLTAAGTQTSIDALAAGAL
                     QIVTKPTMGLKQFLQESAHELIQAVRAAAGARLGALRPRPSAT"
     gene            complement(431..931)
                     /locus_tag="PK144_17480"
     CDS             complement(431..931)
                     /locus_tag="PK144_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNF67566.1"
                     /translation="MSRHAPRDYRGFEWFLMPGDTWFGDGPARVRTLLGSCVAVTLWH
                     PRRHCGGICHFLLPTRRGGRAADVPLDGRYGDEAIELLLREVSGNGTRAAQYQVKLFG
                     GGRMYDTHTQVGTGNVAQARRLVETHGLTLSACHTGGDGHRNLIFDLDTGHVWLRHQP
                     QERARS"
     gene            complement(945..1868)
                     /locus_tag="PK144_17485"
     CDS             complement(945..1868)
                     /locus_tag="PK144_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HNF67567.1"
                     /translation="MNTGSRRQPGAAAAPPGAMRAAGARTPPDTAADSLLSGPGGQIS
                     DAEFEQLARWLYEASGIRLTPAKKALVCGRLGKRLAACGTGSYRAYLTLIQDPRHAAE
                     RQHAIDLLTTNETYFFREPRHFEWLESHVLASPPAGRALRIWSAACSSGEEVYTLAML
                     LAEHFGTRGAWELIGSDLSTRVLASAQAGVYTLERARGIPPALLRRYCLRGVGAQAGR
                     LMIERSLRERAQFRQVNLTEALPDLGLFDVIFLRNVMIYFDVPTKRHVVGQLVRALRP
                     GGHLFIGHSENLHGLCDSLQSVAPSIYRRSS"
     gene            complement(1865..2398)
                     /locus_tag="PK144_17490"
     CDS             complement(1865..2398)
                     /locus_tag="PK144_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNF67568.1"
                     /translation="MSKALTAPRSVAVPAASAESRQFLTFAAGGETLALGILHVREII
                     EYGRVTAVPMLPAFVRGVINLRGSVVPVIDLSARLGRAPSTIGRRSCIVIVELPESDG
                     ERRELGLLVDAVSAVIDIPEIEPPPSFGARMRPDFILGMGKLDERFVVILDPTQTLSL
                     DEMAALAGNLDAGVNGA"
     gene            complement(2418..>2944)
                     /locus_tag="PK144_17495"
     CDS             complement(2418..>2944)
                     /locus_tag="PK144_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNF67569.1"
                     /translation="NLLALNAAIEAARAGEHGRGFAVVAAEVRKLAERSQVASQEIGE
                     LAGSSMAVAEQAGRLLDEIQPAIRRTADLVQEIAAASSEQSTGAGQINSALAQMNQVT
                     QQNASASEELAAAAEELNTQSGQLQQLVGGSAGTGLPVAARSTAGPIARARQAAGALV
                     QGASHDHDGDFVRF"
BASE COUNT          399 a         1008 c         1120 g          417 t
ORIGIN      
        1 gtggcggacg ggcgcgggcg cagcgcgccg agccgggcgc cggcggcggc gcgcacggcc
       61 tggatcagct cgtgcgcgga ttcctgcagg aactgcttca ggcccatcgt cggcttggtg
      121 acgatctgca gcgcaccggc ggcgagcgcg tcgatcgagg tctgcgtgcc ggcggcggtc
      181 agcgtcgagc agatgatcac cggcgtcgga cgctcgctca tcagcttgcg caggaaggtg
      241 atgccgtcca tgcgcggcat ctcgacatcg agcacgatca cgtccggcca ttcgctgcgc
      301 attttctcca gtgcgaacag cggatcgggc gccgcggcga tgacgcggat gtcgggggac
      361 tgggcgaaga cctgcgtgag cacctgccgc acgacggccg agtcgtcgac gatcagcagc
      421 ttgacgggtt tcatgatcgg gctcgttcct gcggttggtg gcgcagccag acgtggccgg
      481 tgtcgagatc gaagatcagg ttgcggtggc cgtcgccacc ggtgtggcag gcgctgagcg
      541 tcaggccgtg ggtttcgacg aggcggcgcg cctgcgcgac gttgccggtg ccgacctggg
      601 tatgggtgtc gtacatgcgc ccgccgccga acagcttcac ctggtactgg gcggcgcgcg
      661 tgccgttgcc gctgacctcg cgcagcagca gttcgatcgc ctcgtcgccg tagcggccgt
      721 cgagcggcac atcggccgcc cggccgccgc ggcgcgtcgg cagcaggaag tgacagatgc
      781 cgccgcagtg gcggcgcgga tgccacagcg tcacggccac gcaggagccg agcagcgtgc
      841 gcacgcgcgc cgggccgtcg ccgaaccagg tgtcgcccgg catcaggaac cactcgaagc
      901 cccggtaatc gcgcggggcg tggcggctca tcggcgggcc tccttcagga actgcgccgg
      961 tagatcgacg gggcgacgga ctgcaggctg tcgcagaggc cgtgcaggtt ctcggagtgg
     1021 ccgatgaaca ggtggccgcc ggggcgcagc gcgcgcacga gctggccgac gacgtggcgc
     1081 ttggtcggca cgtcgaaata gatcatgacg ttgcgcagga agatcacgtc gaacaggccg
     1141 agatcgggca gcgcctcggt caggttgacc tgccggaact gcgcccgctc gcgcaggctg
     1201 cgctcgatca tcagcctgcc ggcctgggcg ccgacgccgc gcaggcagta gcggcgcagc
     1261 agcgccggcg ggatgccgcg ggcgcgctcc agcgtgtaga cgccggcctg cgcgctcgcg
     1321 agcacgcgcg tcgaaagatc agaaccgatc agctcccagg cgccccgggt gccgaagtgc
     1381 tcggccagca gcatcgccag cgtgtagacc tcctcgccgc tcgaacaggc cgcgctccag
     1441 atgcgcagcg cccggccggc cggcgggctg gcgaggacgt ggctttccag ccactcgaag
     1501 tggcgcggct cgcggaagaa gtaggtctcg ttggtcgtca gcaggtcgat cgcgtgctgg
     1561 cgctcggcgg cgtggcgcgg gtcctggatc agcgtcaggt aggcgcggta gctgcccgtc
     1621 ccgcaggcgg cgagccgctt gccgagccgc ccgcagacca gcgccttctt cgccggcgtc
     1681 agcctgatgc cgctggcctc gtacagccag cgcgcgagct gctcgaactc ggcatcgctg
     1741 atctgcccgc cggggccgct gagcaggctg tccgcggcgg tgtccggcgg cgtgcgggcg
     1801 cccgccgcgc gcatggcgcc cggcggggcc gcggcagcgc cgggctggcg acggctgccg
     1861 gtgttcatgc gccgttcacc ccggcatcga ggttgccggc aagcgcggcc atctcgtcga
     1921 gcgacagcgt ctgcgtcggg tcgaggatca ccacgaagcg ctcatcgagc ttgcccatgc
     1981 cgaggatgaa gtccgggcgc atgcgcgcgc cgaagctcgg cggcggctcg atctcgggga
     2041 tgtcgatgac ggcactgacc gcatcgacga gcagaccgag ttcgcggcgc tcgccgtcgg
     2101 actcgggcag ctcgacgatg acgatgcagc tgcgccggcc gatcgtgctc ggcgcacggc
     2161 cgaggcgcgc gctgaggtcg atgaccggca cgacgctgcc gcgcaggttg atgacgccgc
     2221 gcacgaaggc cggcagcatc ggcaccgcgg tcacgcgccc gtactcgatg atctcgcgca
     2281 cgtgcaggat gccgagggcc agcgtctcgc cgccggcggc aaaggtcagg aactgccggg
     2341 attcggcact cgccgccggc acggcgacgg agcggggagc ggtcagggcc ttgctcatgg
     2401 acgcaggtct ccggctctca gaagcgcacg aagtcgccgt cgtggtcatg gcttgcgccc
     2461 tgcaccagcg caccggccgc ctgccgggcg cgggcgatcg ggccggccgt gctgcgggcc
     2521 gcgaccggaa ggccggtgcc ggcgctgccg ccgacgagct gctgcagctg gccggactgc
     2581 gtgttcagct cctcggccgc ggcggcgagt tcctcggagg ccgaggcgtt ctgctgcgtg
     2641 acctggttca tctgcgccag cgcactgttg atctggccgg caccggtcga ctgctccgac
     2701 gaggccgcgg cgatctcctg caccagatcg gcggtgcggc ggatcgccgg ctggatctcg
     2761 tcgagcaggc gcccggcctg ttcggcgacc gccatgctgg agccggcgag ctcgccgatc
     2821 tcctgcgagg cgacctggct gcgctcggcg agcttgcgca cctcggcggc gaccactgcg
     2881 aagccgcggc cgtgctcgcc ggcgcgcgcg gcctcgatcg cggcgttgag cgcgagcagg
     2941 ttgg
//
LOCUS       DAOQIE010000407         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_22744_length_2939_cov_4.825589, whole genome shotgun sequence.
ACCESSION   DAOQIE010000407 DAOQIE010000000
VERSION     DAOQIE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22744_length_2939_cov_4.825589"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK144_17500"
     CDS             <1..258
                     /locus_tag="PK144_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67570.1"
                     /translation="AAPASRPLIVVAGDTAASLGHALQDLPLRLADLDAVLQDGDTLI
                     AVPRAGAAFELRPGRDGAWYVTGTRPAVEATASLKPLFKRR"
     gene            659..1573
                     /locus_tag="PK144_17505"
     CDS             659..1573
                     /locus_tag="PK144_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNF67571.1"
                     /translation="MSSLGILCPGQGAQHAQMLALLAAEPAALAVLAQAEAVLGMAPA
                     TLAAGPELFTNAVAQPLICAAGLATWAALAPKLPPVRVFAGYSVGELTAWACAGALPA
                     ATLIRLASLRAAAMDAACREPTGLLAVRGLDRRQIEALCSAHGCAPAIVNDHDRLVVG
                     GPRAALPGFEAAALALGAQLTPLAVPLASHTPWLAAAVPQFAQALAASAPGTPSAPVL
                     AGIDGSAGFSGTDALRTLPAQIAQTVDWAACTDALVERGCTVLLELGPGSGLSRMARD
                     RHPELAARSVADFRSLDGAAGWVARMLG"
     gene            complement(1691..2554)
                     /locus_tag="PK144_17510"
     CDS             complement(1691..2554)
                     /locus_tag="PK144_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF67572.1"
                     /translation="MMMSESRQTLPPQQQDRQPGLQGAMQPQPDTRPRYPGSGRLRDR
                     VALISGGDSGIGRAVALAFAREGADVTILYFNEHQDAHETVRLIEAEGRQCLAIDGDI
                     GKPAFCRSAVAQTIERFGRLDVLVNNAAEQYPQERIEDISPEQLERTFRTNLYGQFFL
                     IQAALAHLHEGAAIINTTSVTAYHGSPKLLDYSATKGAIVAFTRSLALSLLERGIRVN
                     AVAPGPIWTPLIPATFSADEVASFGAHTPMQRPGQPDEVAASYVFLACADSSYMAGQV
                     LHPNGGQIVNG"
     gene            2750..>2939
                     /locus_tag="PK144_17515"
     CDS             2750..>2939
                     /locus_tag="PK144_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67573.1"
                     /translation="MLARLLCTAALLGLACSSATADLPPAGPSFDCGKVKPDSIAALV
                     WADAQLSALDRKLTAVYAA"
BASE COUNT          372 a         1080 c         1022 g          465 t
ORIGIN      
        1 gcagccccgg catcacgccc gctcatcgtc gtcgccggcg ataccgcagc cagcctcggc
       61 cacgcgctgc aagacctgcc gctgcgcctg gctgatctgg acgcagtgct gcaggacggc
      121 gataccctga tcgccgtgcc gcgcgcaggc gccgcgttcg agctgcgtcc gggccgtgac
      181 ggcgcgtggt acgtgaccgg cacacggccg gcggtcgagg cgactgccag tctcaaaccc
      241 ctgttcaagc gccgttgaat cctgctgcaa tatcgatgga aggcgcggga tttatcgctg
      301 gaaaatcgct ggaaagcgct ggtttttgtc gcaaaatgac cgccgagcac accgcgccca
      361 ggcgccgcta agttgccgtt gtctttgcga ttttttcgct ttttcctttc tttccttgag
      421 tatcaaatca acatccccct gacacggcgt ctcccctctc ccccggcccc tctcccgcaa
      481 ggggagaggg gaagtcgaag ccgaaccgtt ggccgccgat gcatgcagcc cgcggagcgg
      541 ctgatttgtg tagatatcga tgccctctgg gggagggctt ggggtgaggg cggctttcgc
      601 tcgcctgccc ccgtccacca ccctcgggag atagcccgat gcacaccgga cagttgtcat
      661 gagcagcctc ggcatcctct gccccggtca gggtgcgcag catgcgcaga tgctggcgct
      721 gctggctgcc gagccggccg cgctcgccgt gctcgcgcag gccgaagccg tgctcggcat
      781 ggcgccagcc acgctcgccg ccggaccgga gctgttcacg aatgccgtcg cgcagccgct
      841 gatctgcgcc gccggcctcg cgacctgggc ggccctggcg ccgaagctgc cgccggtgcg
      901 cgtgttcgcc ggctacagcg tcggcgaact cacggcctgg gcctgcgccg gggcgctgcc
      961 ggccgccacg ctgatccgcc tcgccagcct gcgcgccgcg gcgatggatg ccgcctgccg
     1021 cgagccgacc ggcctgcttg ccgtgcgcgg gctggatcgg cggcagatcg aggcgctgtg
     1081 ctcggcccac ggctgcgcgc cggcgatcgt caacgaccac gaccggctgg tggtcggcgg
     1141 tccgcgcgcc gcgctgccgg gtttcgaggc cgcagccctc gcgctcggcg cgcaactgac
     1201 gccactcgcg gtcccgctcg cctcgcacac gccgtggctg gccgctgcgg tgccgcagtt
     1261 cgcgcaggcg ctggccgcgt ccgcacccgg cacgccttcg gcgccggtac tcgccggcat
     1321 cgacggctcg gcgggctttt ccggtacgga tgccctgcgc acgctgccgg cgcagatcgc
     1381 gcagaccgtc gactgggccg cctgcaccga cgccctcgtc gagcgcggct gcacggtgct
     1441 gctcgaactc ggccccggca gcggcctgtc gcgcatggcc cgcgaccgcc acccggaact
     1501 cgccgcccgc tcggtcgccg acttccgcag cctcgacggc gcggccggct gggtggcacg
     1561 gatgctcggc tgacaccgcc ggccgccgtg ccggcgcgca ggccgcggcg ggccacccgc
     1621 cgccctcgcc gggcgtggca agagatccgc tgctacgggg ccggaccggc gtccggcccc
     1681 gggccggact tcagccgttg acgatctgcc cgccgttcgg gtgcagcacc tgcccggcca
     1741 tgtacgagct gtcggcgcag gcgaggaaca cgtagctggc cgcgacctcg tcgggctggc
     1801 cggggcgctg catcggcgta tgggcgccga agctcgcgac ctcgtcggcg ctgaaggtcg
     1861 ccggaatcag cggcgtccag atcggaccgg gcgccacggc gttgacgcgg atgccgcgtt
     1921 cgagcagcga cagcgccagc gagcgcgtga aggcgacgat cgcgcccttg gtcgccgagt
     1981 agtcgagcag cttcggactg ccgtggtagg cggtcaccga cgtggtgttg atgatcgccg
     2041 cgccttcgtg caggtgcgcg agcgcagcct ggatcaggaa gaactgcccg tagaggttgg
     2101 tccggaaggt gcgttcgagc tgctcggggc tgatgtcctc gatgcgctcc tgcggatact
     2161 gctcggcggc gttgttgacc agcacgtcga gccgcccgaa gcgttcgatc gtctgcgcga
     2221 ccgcgctgcg gcagaacgca ggcttgccga tgtcgccgtc gatcgcgaga cactggcggc
     2281 cttcggcctc gatcagccgc acggtctcgt gcgcgtcctg atgctcgttg aagtacagga
     2341 tcgtgacatc ggcgccctcg cgcgcgaacg ccagcgcgac cgcgcggccg ataccgctgt
     2401 cgccgccgct gatcagcgcg acccggtcac gcagccggcc gctgccgggg tagcgcggcc
     2461 gggtatcggg ctgcggctgc attgcgccct gcagtccggg ctgccggtcc tgctgctgcg
     2521 gcggcagcgt ctgacgggat tcgctcatca tcatctgacc tcttgctctg cccgcggggg
     2581 gacggacagg gtttcgaggc cgggatcgcg gcggggttcg gattttctcc cggaacatcg
     2641 ggttgccgcc gggaacggca aggccggaca gccgccggcc gctggctaca gtgaggccgc
     2701 ttccccccgg aaagaccgca tccgccgccc tcgccctccg gagtccccga tgctcgcccg
     2761 cctcctttgc accgccgccc tgctcggcct cgcctgcagc agcgcaaccg ccgacctgcc
     2821 gcctgccggt ccgtccttcg actgcggcaa ggtcaaaccc gacagcatcg cggcactggt
     2881 ctgggccgat gcgcagttgt cggcgctcga tcgcaagctg accgcggtct acgccgccg
//
LOCUS       DAOQIE010000408         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23039_length_2918_cov_6.551519, whole genome shotgun sequence.
ACCESSION   DAOQIE010000408 DAOQIE010000000
VERSION     DAOQIE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23039_length_2918_cov_6.551519"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK144_17520"
     CDS             <1..475
                     /locus_tag="PK144_17520"
                     /inference="COORDINATES: protein motif:HMM:NF023592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HNF67574.1"
                     /translation="VAPPPTVVPAAVTAAPEAAPVAAAVVTDALPDWPALVARLPVKG
                     RPLQLARHAVLEAVQHDRWTLALDPAHASLQSTAGEQKLAEALTSALGRPVTLMLRVG
                     AAGLATPARAEDAAAAGRRQAAIEAFGQNPAVRALVETLDARIRSDSITPGPAAP"
     gene            564..890
                     /locus_tag="PK144_17525"
     CDS             564..890
                     /locus_tag="PK144_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNF67575.1"
                     /translation="MMKGGLGNLMKQAQQMQEKLQKAQAEIAAMEVEGESGAGLVRVV
                     ITGRHEARRVTIDPSLLADDKDMLEDLVAAAFNDAVRKLERTTQERMSGLTAGLPLPP
                     GMKLPF"
     gene            922..1521
                     /gene="recR"
                     /locus_tag="PK144_17530"
     CDS             922..1521
                     /gene="recR"
                     /locus_tag="PK144_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNF67576.1"
                     /translation="MSAYPRKLAQLIEALRCLPGVGPKSATRMAFHLVERDRAGAGRL
                     AAALTAAVAGIGRCERCRNLAEGPLCAGCADPQRDDSLLCVVETPADVAAIEHATGFR
                     GRYFVLMGRLSPLDGIGPEELGLDRLDARLAEGTVQELILATNPTVEGEATAHYLAQL
                     AHARGVQATRIAHGVPLGGELEHVDGGTLAHALAGRRTV"
     gene            1537..2166
                     /locus_tag="PK144_17535"
     CDS             1537..2166
                     /locus_tag="PK144_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNF67577.1"
                     /translation="MQPDLFAGDDDGSGECIELPDAALEYRPAFLNASEAAAAFDELR
                     ATLAWRQDVLRFGGREVPIPRLNAWYGEAGTTYTYSGLRLEALPWTETLAALRARIEA
                     ASGARFDSVLANLYRDGRDSVAWHADDEPELGRDPVIASLSLGAVRSFELRHRRARAL
                     GLGTQRLALADGSLLVMRGSTQHHWLHRVPKEPAVTAARINLTFRLIRR"
     gene            complement(2350..2862)
                     /locus_tag="PK144_17540"
     CDS             complement(2350..2862)
                     /locus_tag="PK144_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNF67578.1"
                     /translation="MIRRILVLASLLLSSVTAQAVLISRTLEITATDFVIAFGAPQIP
                     AVSPVNIRFSVTFDNSTDIGPTTAGLTVQHFNLPYPVEYAYSTAQDVLSLASDPFVNG
                     CSHPVSSFCIIVDAFSLASPVAFFVAQSLEPDAGWHALTIHTSVIPEPAPLLLFGSVL
                     PGLVAVRRRR"
BASE COUNT          476 a         1093 c          979 g          370 t
ORIGIN      
        1 ggtcgcaccg cccccgaccg tcgtgcccgc cgccgtcacc gcggcgccgg aagcggcccc
       61 ggtcgcggcg gccgtcgtca ccgatgccct gcccgactgg ccggcgctgg tcgcgcggct
      121 gccggtcaag ggccggccgc tgcagcttgc gcggcacgcg gtgctcgaag ccgtgcagca
      181 cgaccgctgg acgctcgcgc tcgatccggc gcacgcgagc ctgcagtcaa cggccggcga
      241 gcagaagctc gccgaagccc tgaccagcgc gctcgggcgg ccggtgacgc tgatgctgcg
      301 cgtcggcgcc gccgggctcg cgacgccggc gcgcgccgag gacgccgcgg ccgccggtcg
      361 ccggcaggcg gcgatcgaag ccttcggaca gaacccggcc gtgcgcgcgc tggtcgagac
      421 cctggatgcg cgcatccgca gcgacagcat cacgcccggc ccggccgccc cctgaacgcg
      481 cagcgacccg catgcccggt acactgcggc agcgatcgaa tctcccctcc cccgcacgca
      541 cgacctacca gaggacagca gccatgatga agggcggact cggcaacctg atgaagcagg
      601 cgcagcagat gcaggaaaag ctgcagaagg cgcaggcaga gatcgccgcg atggaggtcg
      661 agggcgaaag cggcgccggc ctggtcaggg tcgtcatcac cggccgccac gaggcgcggc
      721 gcgtgaccat cgacccctcg ctgctcgccg atgacaagga catgctcgaa gacctggtcg
      781 ccgcggcctt caacgacgcg gtgcgcaagc tcgaacgcac gacgcaggag cgcatgtcgg
      841 gcctcaccgc cggcctgccg ctgccgcccg gcatgaagct gccgttctga gcccggccgg
      901 cccgcagccc ggcgcgcacg catgagcgcc tacccgcgca agctcgcgca gctgatcgag
      961 gcgctgcgct gcctgcccgg tgtcggtccg aaatcggcga cgcgcatggc ctttcacctc
     1021 gtcgaacgcg accgcgccgg tgccggccgg ctggccgcgg cgctgacggc cgcggtcgcc
     1081 ggcatcggcc gctgcgaacg ctgccgcaac ctcgccgaag gcccgctgtg cgccggctgc
     1141 gccgatccgc agcgcgacga cagcctgctc tgcgtcgtcg agaccccggc cgatgtcgcc
     1201 gccatcgagc acgcgaccgg cttccgcggc cgctacttcg tgctgatggg ccggctgtcg
     1261 ccgctcgatg gcatcggccc cgaggaactc ggcctcgacc gcctcgatgc gcgcctcgcc
     1321 gaaggcacgg tgcaggaact catcctcgcc accaacccga ccgtcgaggg cgaggccacc
     1381 gcgcactatc tggcgcagct tgcgcacgcg cgcggcgtgc aggcgacgcg catcgcccac
     1441 ggcgtgccgc tcggcggcga gctcgaacac gtcgatggcg gcacgctcgc ccacgcgctc
     1501 gccggccggc gcacggtctg acgcgccggc cccccgatgc agcccgacct gttcgccggc
     1561 gatgacgatg gctctggcga gtgcatcgaa ctgcccgacg ccgcgctcga ataccgcccc
     1621 gccttcctga acgcgagcga agccgccgcc gccttcgacg aactgcgcgc cacactcgcg
     1681 tggcgacagg acgtgctgcg cttcggcggg cgcgaagtgc cgatcccgcg cctgaacgcc
     1741 tggtacggcg aagccggcac gacctacacg tactcgggcc tgcgcctcga agcgctgccg
     1801 tggacggaaa cgctcgccgc gctgcgcgca cgcatcgagg ccgcgagcgg cgcgcgcttc
     1861 gacagcgtgc tcgccaacct ctaccgcgac ggccgcgaca gcgtcgcctg gcacgccgac
     1921 gacgaacccg aactcggccg cgacccggtc atcgcctcgc tgtcactcgg cgccgtgcgc
     1981 agcttcgaac tgcgccaccg ccgcgcccgc gccctgggcc tcggcacgca gcgcctcgcg
     2041 ctggccgacg gcagcctgct ggtcatgcgc ggcagcacgc agcatcactg gctgcaccgc
     2101 gtaccgaagg aacccgccgt caccgccgcg cgcatcaacc tgaccttccg gttgatccgg
     2161 cgctgagagc cggtatctac gagttgctcg ctacacggct gcccgatgcc cggcacagca
     2221 catcctcgcc atacaactcg atcgtcgcct gcattaccgc gctccgcacg tagcaaccct
     2281 gacgggcgga tagcgatgcc acagtgcggc gcgcaatgcc agtccgccgg aggcgccggc
     2341 tgcagccgac tacctgcgcc tgcgcacagc caccagcccc ggcaggacgg aaccgaacag
     2401 cagcagcggc gccggttcag gaatgacgga tgtgtggatc gtcagcgcat gccagccggc
     2461 gtcgggttcg agtgattgcg ccacgaagaa ggcaaccgga ctggccagac tgaaggcatc
     2521 gacgatgatg cagaacgagg acacgggatg agagcagcca ttgacgaacg gatcgctggc
     2581 gagactgagc acgtcctggg cagtcgaata ggcatattcg accggatacg gcaggttgaa
     2641 atgctggacc gtcaggcccg cggtcgtcgg gccgatatcc gtcgaattgt cgaaagtcac
     2701 cgagaaacgg atgttcaccg gcgaaaccgc cggaatctgg ggtgccccga acgcgatgac
     2761 gaaatccgtg gcggtgattt ccagagtccg cgaaatgagc acggcctgcg ccgtgacgga
     2821 tgacagcaac agcgatgcga gcacaaggat tcttctgatc atctgcctgc tcctctgatt
     2881 cccggttttt catggctgcc ggtgaagccg ccccggcc
//
LOCUS       DAOQIE010000409         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23220_length_2905_cov_4.996140, whole genome shotgun sequence.
ACCESSION   DAOQIE010000409 DAOQIE010000000
VERSION     DAOQIE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23220_length_2905_cov_4.996140"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2808
                     /locus_tag="PK144_17545"
     CDS             <1..2808
                     /locus_tag="PK144_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF67579.1"
                     /translation="LRLFGNVGAVFNLQHWPDTSLSFWIRLWTLAWCGPYAPYSSDAQ
                     AFIGKNHEPVWSMLRARPAGRLLTLWHDALRNWLTRQQPATVAPADRRGLWQRMQMAH
                     ALLALDGAAAGDGFAGLYRKLAAGIAAIEQPRQQERRRLDAQVRLGLERLQAPGSLEV
                     ADSAAVRNWLADWLNRLSPWQRPFLSGRSRQLLALLDQRQALAAAGYGSAAQIAQHRR
                     RLAACETAVIDWIAGQVLRHCGLPAALGEPATVALGLLFTSRHEAADALLMRWQQQAP
                     WQSLPACAEAWPATQPQHWTVFDDGARAALYVWLQHWPELKVDDQLANGAGSDLDAAL
                     RAWADGDAQPLCGLLHGGWQAAQEPADKLGRCLSALQPALTAPDLRACSEGPAAVDHA
                     VRIDALAAVLCGRAQARLAQALRDWLDAQCPLPAEGLDDALLARVRQRFSRLCSLHPG
                     PPAQHELGERLHGWAQQWLGDQLAEARPQPEAIWQTLELARIGLNGLRPSLPDADWHE
                     ATAEAVREALLNTKALPPGPGLFWPPLQRWFGRLDAPGGLPRLPEVETCRRRLRTGEA
                     LVQVLFDPGSGRQLALWLDTRQPLRLRAFPAPANHAAWREGALALWQQWAADEHSQGL
                     ARLWPTLIADPAVQVTAATLAGWAAEAGLERLIVIWPASLAQLPWEVFAPFVDGAPVL
                     ERAVNLGNWRTASAREPAKAWVAHFADSAHGGFAIEAMAADSARFWQVDAQPCGIQAA
                     SGFDVLGLLHGGGAGHLIVHGEYRPDEPRLSRLQIATGEYLPAWTLGALGIGCPLLWL
                     SACQANLSGRATRERLGPVGLGPTLIAAGAQRVLGALWKCDTLAAWLLHRFLHAAIAE
                     AAGASPQARYWHALREARERLRALRGSEVEALLRAELGEAVFGERRYEFDDYLLPARP
                     FDNPYYWAAFMLLGEPDAVAG"
BASE COUNT          385 a         1009 c         1086 g          425 t
ORIGIN      
        1 ctccgactgt tcggtaacgt cggtgctgtg ttcaatctgc agcactggcc ggacaccagc
       61 ctgagcttct ggattcggct gtggacgctg gcctggtgcg gcccgtatgc accgtacagt
      121 tccgatgcgc aggctttcat cgggaagaac cacgagccgg tgtggtcgat gctgcgtgcc
      181 cgccccgccg gccggctgct gacgctctgg cacgacgcgc tgcgcaactg gctgacccgt
      241 cagcagccgg cgacggtggc gcctgcggat cgccgggggc tgtggcagcg catgcagatg
      301 gcgcatgcgc tgctggcgct cgatggcgcg gccgccggcg atggttttgc cgggttgtac
      361 cggaagctgg ccgccgggat cgccgccatc gaacagccac ggcagcaaga gcgccgccgg
      421 ctcgatgcgc aagtgcgact cggactggaa cgcctgcagg cccccggatc gctggaggtc
      481 gcggattcgg cagcggtgcg caactggctt gccgactggc tgaaccggct cagcccctgg
      541 cagcggcctt ttctctcggg ccgcagcaga caactgctgg ccctgctgga tcagcggcag
      601 gcgctggcgg cagcgggcta cgggtcggcg gcgcagatcg cgcagcaccg ccgccggctg
      661 gcggcttgcg agacggccgt catcgactgg atcgccgggc aggtgctgcg ccattgcggt
      721 ctgccggcag cactcggcga accggcgacg gttgcgctcg gcctgctgtt cacgagccgg
      781 cacgaagcag cggatgcgct gctgatgcgc tggcagcagc aggcgccgtg gcagtcgttg
      841 cctgcgtgcg ccgaagcctg gccggcgacg cagccgcagc actggaccgt gttcgacgat
      901 ggcgcacgcg cggcactgta cgtctggctg cagcactggc cggagctgaa ggtcgacgat
      961 caactggcca atggcgccgg gtcggacctc gatgccgcat tgcgcgcgtg ggcagacggt
     1021 gatgcgcaac cgctgtgtgg tctgctgcat ggcggctggc aggcggcgca ggaaccggcg
     1081 gacaagctcg gccgctgcct gtcggcgctg cagcccgccc tcacggcgcc cgatctgcgc
     1141 gcctgcagcg aaggaccggc cgctgtggat cacgcggtgc ggatcgacgc gctggcggcc
     1201 gtgctgtgcg gccgggcgca ggcgcggctg gcgcaggcgc tgcgtgactg gctcgatgcg
     1261 caatgcccgc tgccggccga aggactggac gatgcgctgc tggcgcgtgt gcgccagcgc
     1321 ttctcgcggc tgtgcagcct gcaccccggt ccgccggcgc agcacgaact cggcgagcgc
     1381 ctgcacggct gggcgcagca atggctcggc gaccagcttg ccgaggcccg gccgcagcct
     1441 gaagcgatct ggcagacgct cgaactcgcg cgcatcggcc tgaacggctt gcgcccgtcg
     1501 ctgcccgatg ccgactggca cgaagccacg gccgaagccg tgcgcgaggc gctgcttaac
     1561 accaaggcac tgccaccggg gccaggactg ttctggccgc cgctgcagcg ctggttcggg
     1621 cggctcgacg cgcccggcgg cctgccgcgc ttgccggagg tcgaaacctg tcgccggcgc
     1681 ctgcgcaccg gcgaagcact ggtgcaggtg ttgttcgatc ccggcagcgg ccggcaactg
     1741 gcgctgtggc tcgatacccg gcagccgctg aggctgcggg cgtttccggc gccggcgaat
     1801 cacgccgcct ggcgcgaagg cgcgctggcg ctctggcagc agtgggcggc agacgagcac
     1861 agccagggtc tggcccggct gtggccgacg ctgatcgccg atccggccgt gcaggtcacc
     1921 gctgccacgc tggccggctg ggcggccgaa gccggactcg aacgcctgat cgtgatctgg
     1981 ccggcatcgc tcgcgcagtt gccgtgggag gtgttcgcgc ccttcgtcga cggtgcgccg
     2041 gtgctggagc gggcggtgaa tctcggcaac tggcgcacgg cgtcggcccg tgaaccggca
     2101 aaggcctggg tcgcgcattt cgccgattcc gcccacggcg gctttgcgat cgaggcgatg
     2161 gccgccgaca gtgcccgctt ctggcaggtg gacgcgcagc cctgcggtat ccaggccgca
     2221 agcggcttcg atgtgctggg actgctgcac ggcggcgggg ccgggcatct gatcgtgcac
     2281 ggcgaatacc ggccggacga accgcgcctg agccggctgc agatcgccac cggcgagtac
     2341 ctgccggcct ggacgctcgg cgcgctcggc atcggctgcc cgctgctgtg gctgtcggcc
     2401 tgtcaggcca acctcagcgg ccgggcgacg cgcgagcggc tcggcccggt cggcctcggt
     2461 ccgacgctga tcgcggccgg cgcgcagcgc gtactcgggg cgctgtggaa atgcgacacg
     2521 ctggcggcct ggctgctgca tcgattcctg cacgcagcca tcgccgaagc cgccggggca
     2581 tccccgcagg cgcggtactg gcacgcgctg cgcgaggccc gcgagcggct gcgcgcactg
     2641 cgcggcagcg aggtcgaagc gctgctgcgc gcggaactgg gcgaagcggt gttcggcgag
     2701 cgccgctacg aattcgacga ctacctgctg cccgcacggc cgttcgacaa cccgtactac
     2761 tgggccgcct tcatgctgct cggcgaaccg gatgccgtgg caggctgacc gggccgaacc
     2821 gccgccgcgt aaaacttgcc acgtggcaag ttccgccgaa gcggatcggc cgccgccggc
     2881 gcgacgctat cccgatgcgc gcgcc
//
LOCUS       DAOQIE010000410         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23248_length_2903_cov_6.939256, whole genome shotgun sequence.
ACCESSION   DAOQIE010000410 DAOQIE010000000
VERSION     DAOQIE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23248_length_2903_cov_6.939256"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PK144_17550"
     CDS             complement(<1..238)
                     /locus_tag="PK144_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF67580.1"
                     /translation="MSETPVTVTVNGVEIAETDIQTELQHHPLPDPAQARQAAATALV
                     MRELLLQEARSHGIDTADTDTAIATLIERAVQLPQ"
     gene            complement(287..964)
                     /locus_tag="PK144_17555"
     CDS             complement(287..964)
                     /locus_tag="PK144_17555"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNF67581.1"
                     /translation="MPDISAFVSGFLKFQAEHFGEDGLFETLRHGQHPSTLLIGCCDA
                     RVDPALLTGCKPGDIFIVRNVANLVPPCTPDAPPGVSSAIEFAICGLGVERIIVLGHA
                     RCGGIRYLMDPQPLTDETDFLGRWMQIAEPARTRVLHELAHKSPEERLRALELASILL
                     SLDNLMSYPWLRRRVEAGGLTLHGWYFDIAEGALLAYSARRQQFLPVVCPLDRSTHRK
                     YDAGPTP"
     gene            complement(1034..1714)
                     /gene="narI"
                     /locus_tag="PK144_17560"
     CDS             complement(1034..1714)
                     /gene="narI"
                     /locus_tag="PK144_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNF67582.1"
                     /translation="MSYLNDLLFGVYPYIAGTVFLIGSLLRFDRDQYTWKAHSSQMLS
                     NAGFRRASNLFHIGIIGIFFGHLAGLLTPVAVWHALGVSASFKQWVAIVLGGLFGTMC
                     FIGLTALVHRRLNDARVRANSSRMDIVILLLLYGQLILGLLTIPVSLFHLDGANMLTL
                     MAWAQHIVTFDGPGAAAIAANVGGLFKLHLLLGMTIFLLFPFSRLVHVWSVPLGYVKR
                     PYQIVRQR"
     gene            complement(1748..2470)
                     /gene="narJ"
                     /locus_tag="PK144_17565"
     CDS             complement(1748..2470)
                     /gene="narJ"
                     /locus_tag="PK144_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNF67583.1"
                     /translation="MLTLKIVSALLCYPQPDLMDALPELAALVREDDELPVVERGRLV
                     DFIDELADSDLWTLQEHYVARFDRGRKLSLHLFEHIHGESRDRGQAMVDLLDVYRGAG
                     FELKARELPDYLPLFLEFLATRPHEEARRLLDDASHVIALLGARLAERHDPYAVLFDA
                     LLALIELPADLGEIRAQAASEGPDRTITDMDKIWEEEQVTFLASQAPDGGCGVAAAGT
                     AQPLAFVPPRQMRAPAPSQPSR"
     gene            complement(2499..>2903)
                     /gene="narH"
                     /locus_tag="PK144_17570"
     CDS             complement(2499..>2903)
                     /gene="narH"
                     /locus_tag="PK144_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258540.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNF67584.1"
                     /translation="DSLRIPVRYLANLLTAGNEPPVTLALKRMLAMRAYMRAKTVDGE
                     IRQDVLDSVGLRLDQVESMYRYLAIANYEDRFVIPSSHREYASATYDAFSERGGCGFS
                     FGNGCADGGSKLNLFGGKKQTTRRANPIRFVK"
BASE COUNT          457 a          935 c         1070 g          441 t
ORIGIN      
        1 cctgcggcag ttgcacggcg cgctcgatca gcgtcgcgat cgcggtatcg gtgtcggcgg
       61 tgtcgatgcc gtggctgcgg gcctcctgca gcagcagctc gcgcatcacc agcgccgtcg
      121 ccgcggcctg acgcgcctgc gccgggtcgg gcagcgggtg gtgctgcagt tcggtctgga
      181 tgtcggtttc ggcaatctcg acaccgttga cagtcacggt cacgggggtc tcgctcatgg
      241 cggctctcgg ggggcggggc ggaggcgggc ggtcgcggcg gccgggtcag ggcgtcgggc
      301 cggcgtcgta cttgcggtgc gtgctgcgat cgagcgggca gaccacgggc aggaactgct
      361 ggcggcgcgc agagtaggcg agcagcgccc cttcggcgat gtcgaaatac cagccgtgca
      421 gcgtcagccc gccggcctcg acgcgccggc gcagccacgg gtagctcatc aggttgtcga
      481 gcgacagcag gatgctcgcc agctccagcg cgcgcaggcg ctcctcgggc gacttgtgcg
      541 cgagttcgtg cagcacgcgc gtgcgtgccg gttcggcgat ctgcatccag cggccgagga
      601 agtcggtctc gtcggtcagc ggctgcgggt ccatcaggta gcggatgccg ccgcagcgcg
      661 catggccgag cacgatgatg cgctcgaccc cgaggccgca gatcgcgaac tcgatcgccg
      721 agctgacgcc gggcggcgcg tccggcgtgc acggcggcac gaggttggcg acgttgcgca
      781 cgatgaagat gtcgccgggc ttgcagccgg tcagcagcgc cggatcgacg cgggcgtcgc
      841 agcagccgat cagcagcgtc gacgggtgct ggccgtggcg cagcgtctcg aacaggccgt
      901 cctcaccgaa gtgctcggcc tggaacttca ggaagccgga aacgaaggcg gagatgtcag
      961 gcatggcggc gctaccgggc aagacggcgg ggaaggcggc ttgcgcgtct tccccgcccg
     1021 gacaggcggc cgttcagcgc tggcgcacga tctggtacgg gcgcttcacg tagccgagcg
     1081 gcacgctcca gacgtgtacg aggcggctga acgggaacag caggaagatc gtcatgccga
     1141 gcagcaggtg cagcttgaac agcccgccga cgttggcggc gatcgccgcg gcgcccgggc
     1201 cgtcgaaggt gacgatgtgc tgcgcccagg ccatcagcgt cagcatgttg gcaccgtcga
     1261 ggtggaacag cgacaccggg atggtcagca ggccgaggat cagctgcccg tacagcagca
     1321 gcaggatcac gatgtccatg cggctcgaat tcgcacgcac gcgcgcatcg ttcaggcgcc
     1381 ggtgcaccag cgccgtcagg ccgatgaagc acatcgtgcc gaacaggccg ccgagcacga
     1441 tcgccaccca ctgcttgaag ctcgccgaca cgccgagcgc gtgccagacc gcgaccggcg
     1501 tcagcaggcc ggccagatgg ccgaagaaga tgccgatgat gccgatgtgg aacaggttgc
     1561 tggcgcgccg gaagccggcg ttcgacagca tctggctcga atgcgccttc cacgtgtact
     1621 ggtcgcggtc gaagcgcagc aggctgccga tcaggaacac ggtgccggcg atgtacgggt
     1681 agacgccgaa cagcaggtcg ttcaggtagg acatgacgga attcctcagg attcaggagc
     1741 cggcggctca gcgggacggc tgcgaaggcg ccggcgcgcg catctgccgc ggcggcacga
     1801 aggccagcgg ttgcgccgtg ccggccgccg cgacgccgca gccgccgtcc ggcgcctggc
     1861 tggcgaggaa ggtcacctgt tcctcctccc agattttgtc catgtcggtg atcgtgcggt
     1921 ccgggccttc gctggcggcc tgtgcgcgga tctcgccgag gtctgcgggc agctcgatca
     1981 gcgccagcag cgcgtcgaac agcaccgcgt acgggtcgtg gcgctcggcc aggcgcgcac
     2041 cgagcagcgc gatgacgtgg ctcgcgtcat cgagcaggcg gcgcgcctcc tcgtgcgggc
     2101 gcgtggccag gaattcgagg aacagcggca ggtagtccgg cagctcccgg gccttcagct
     2161 cgaagccggc gccgcggtag acatcgagca gatcgaccat cgcctgaccg cggtcgcgcg
     2221 attcgccgtg gatgtgctcg aacaggtgca ggctcagctt gcggccgcgg tcaaagcgcg
     2281 cgacgtagtg ctcctgcagc gtccagaggt cggaatcggc cagttcgtcg atgaagtcca
     2341 ccagccggcc gcgctcgacg accggcagtt catcgtcctc gcgcaccagt gcggccagtt
     2401 cgggcagggc gtccatgaga tccggctgcg ggtagcagag cagcgcggac acgatcttca
     2461 gcgtcagcat gatgcgtatt cctcgggtgt ccggtgcttc acttgacgaa gcggatcggg
     2521 ttggcgcggc gggtcgtctg ctttttgccg ccgaagagat tcagcttgct gccgccatcg
     2581 gcgcagccgt tgccgaagct gaagccgcag ccgccacgct cgctgaacgc gtcgtaggtc
     2641 gccgacgcgt attcgcggtg cgagctcgga atcacgaagc ggtcctcgta gttcgcgatc
     2701 gcgagatagc ggtacatgct ctcgacctga tcgaggcgca ggccgacgct gtcgagcacg
     2761 tcctggcgga tttcgccgtc gacggtcttg gcgcgcatgt aggcacgcat cgcgagcatg
     2821 cgcttcagcg ccagcgtcac cggcggttcg ttgccggcgg tcagcaggtt ggcgaggtag
     2881 cgcaccggaa tgcgcaggct gtc
//
LOCUS       DAOQIE010000411         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23336_length_2898_cov_2.918748, whole genome shotgun sequence.
ACCESSION   DAOQIE010000411 DAOQIE010000000
VERSION     DAOQIE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23336_length_2898_cov_2.918748"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1670
                     /locus_tag="PK144_17575"
     CDS             66..1670
                     /locus_tag="PK144_17575"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HNF67585.1"
                     /translation="MSREAVLKALADAVDADDVLRKRPAPTASVDDVLPLRRPLPPPE
                     PFPLDALGDLLGRAAERMRDVIQAPPALCGASVLAAASLAVQALADVVIDGRRMPCSL
                     FAVTIGESGERKSAVDAVALRAVDAFQRELAEAQAGACAIHENDLAVWRLARDAALRP
                     PSSRKGEPRSVDREAVRAALDDMGPEPLPPLLPLIRAGDPTHEGLYKLLAAGRPSIGL
                     FSDEGGQFVGGHAMNADNALKTAAGLSKLWDGRPLDRVRGGDGASLLYGRRVALHLMM
                     QPAVADLLLGNGLIEGQGFLARCLICWPDSTAGTRLYQATDLSADSTVAAYGARITDR
                     LRAALPLAEGRRNELEPRALPLADGAKRLWTAFHDAIEARLATDGDLAPIRAFASKLA
                     EHAARIAGVLTLFEAGHATEIDLDAMRSGIRVADFYAGERLRIASAGRTDPEIDLAER
                     LLAWMEATRDRRRDRSDCGLFHLAELYQGGPNAMRDAKTARRIVGVLVTHGYAVAEPD
                     GARLPDLKGREAHRREVWRLLTRADT"
     gene            1667..2419
                     /locus_tag="PK144_17580"
     CDS             1667..2419
                     /locus_tag="PK144_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67586.1"
                     /translation="MILFDLDALECAAAQKSATAANPAKPANRPLAAAPDPLPISGIS
                     GISDAGGFCFPERSQPTAPAAVLLAGADQEPVGHCAACGSPSWWRAADGADWCCSYCS
                     PRPQPFTGTSLTLACGWRAGTAPPADPDDEPAPAVRPADGRVQNAYGAWRLPAEHAAV
                     EDYDRHHWQCTVCRRAGSGYGDRCAEGERLHAATRIALSEAIQGRMVGRVGNPSLKAN
                     SGNISGIERGDSRDIAARAAGLGSGKTLEGAP"
     gene            2500..2700
                     /locus_tag="PK144_17585"
     CDS             2500..2700
                     /locus_tag="PK144_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67587.1"
                     /translation="MIEALSETRRERDAARREAEQQRLRATALLHHLERARALLARPS
                     RPRPRAGVPSQLPLTLPSRTDR"
     gene            2697..>2898
                     /locus_tag="PK144_17590"
     CDS             2697..>2898
                     /locus_tag="PK144_17590"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HNF67588.1"
                     /translation="MTSATTRRARVDAVLAELRERFPAAFPTDLSTVRPLALGIREAI
                     EALKIAGRTPVHRVLWLWTRTPA"
BASE COUNT          404 a         1070 c         1030 g          394 t
ORIGIN      
        1 gtgcgcatcg cgctgccggt cgaggccggc agcgacttcg ccgacgtgct cgccgaggtg
       61 cagccatgag ccgcgaagcc gtattgaagg cgctggccga tgccgtcgac gccgatgacg
      121 tgctgcgcaa gcggccggca ccgactgcga gcgtcgatga cgtgctgccg ctgcgccgcc
      181 cgctgccacc gcccgagccg ttcccgctcg acgcgctcgg cgacctgctc gggcgggccg
      241 ccgagcgcat gcgcgacgtg atacaggcac cgcccgcact gtgcggggca tcggtgctgg
      301 ccgccgcatc cctcgccgtg caggctctgg ccgacgtagt gatcgacggc cgccggatgc
      361 cgtgctcgct gttcgccgtg accatcggcg aatccggcga acggaaatcg gccgtcgatg
      421 ccgtcgcgct gcgcgccgtc gatgcgttcc agcgcgagct ggccgaggcg caggccggcg
      481 cgtgcgccat ccacgagaac gacctcgccg tctggcgact ggcgcgcgat gccgcactgc
      541 gaccgccatc gagccgcaag ggcgagccgc gcagcgtgga ccgcgaggcc gtgcgggcgg
      601 cactggacga tatgggtccg gaaccgctgc cgccgctgct gccgctgatc cgggcgggcg
      661 atccaacgca cgaggggctt tacaagctct tggccgccgg gcgaccgagc atcggcctgt
      721 tcagtgatga aggcgggcag tttgtcggcg ggcacgccat gaacgccgac aacgcgctga
      781 aaaccgctgc cggcctgtcg aagctctggg acggccggcc gctggaccgc gtgcgcgggg
      841 gcgacggtgc atcgctgctg tacggccggc gcgtggcgct gcacctgatg atgcagccgg
      901 ccgtcgccga tctgctgctc ggcaatgggc tgatcgaggg gcaaggcttc ctcgcgcgct
      961 gcctgatctg ctggccggac tcgacggccg gcacccggct gtatcaggcc acggacctgt
     1021 cggcagactc gaccgtcgcc gcctacggcg cccggatcac cgaccgcctg cgggccgcgc
     1081 tgccgctcgc cgagggccgg cgcaacgagc tggagccgcg cgcgctgccg ctcgctgatg
     1141 gcgccaagcg gctgtggacc gccttccacg acgccatcga ggcccggctc gccacggatg
     1201 gcgacctggc accgatccgc gccttcgcct cgaagctcgc cgagcacgcc gcccgcatcg
     1261 ctggcgtgct gacgctgttc gaggccgggc acgccaccga gatcgacctc gacgccatgc
     1321 gcagcggcat ccgcgttgcc gacttctacg ccggcgagcg actgcgcatc gcttcggcgg
     1381 gccgcaccga tcccgagatc gacctcgccg agcggctgct cgcgtggatg gaggcgacac
     1441 gggatcggcg cagggaccgc agcgactgcg gcctgttcca cctggccgag ctgtatcaag
     1501 gggggccgaa cgccatgcgc gacgccaaga cggcgcggcg catcgtcgga gtgctggtca
     1561 cgcacggcta cgccgtggcc gagccggacg gcgcccgcct gccggacctg aagggccgcg
     1621 aagcgcaccg gcgcgaggtg tggcgtctgc tcacgagggc cgacacatga tcctgttcga
     1681 cctcgacgcg ctcgaatgcg ctgccgcgca gaaatctgcc acggccgcta atcccgctaa
     1741 accggctaat cggccgctcg cagccgcgcc cgatccgctg ccgattagcg ggattagcgg
     1801 gattagcgat gctggcggat tttgttttcc cgagcgcagc cagccgaccg ccccggccgc
     1861 tgtgctgctc gccggtgccg atcaggagcc ggtcgggcac tgcgcggcct gcggcagtcc
     1921 gtcgtggtgg cgagctgcag acggcgccga ctggtgctgt tcgtactgct cgccgcgacc
     1981 gcagccgttc accggcacgt cgttgacgct ggcctgcggc tggcgagccg gcaccgcgcc
     2041 gccggccgat ccggacgacg aaccggcgcc ggccgtccgg cctgcagacg ggcgcgttca
     2101 gaacgcctac ggggcatggc gcctgccagc cgagcacgca gccgttgagg actacgaccg
     2161 ccaccactgg cagtgcaccg tctgccgccg cgctggtagc ggctacggcg accgctgcgc
     2221 cgagggcgag cggctgcacg ctgctacgag gatcgcgctg tccgaggcga ttcaggggcg
     2281 gatggtcggg cgggtcggca acccgagcct gaaggccaat tccggaaata tttccggaat
     2341 tgagcggggc gactcccgcg acatcgccgc ccgcgccgct ggccttggtt ccggcaagac
     2401 gctggagggg gcgccgtgaa gccgtggagg cccgccccgc ctgatcgcgc caccgagcgc
     2461 gcacacgatc ccgaatccct gactgccagc accggccgct tgatcgaggc cctgagcgaa
     2521 acccggcgcg aacgcgatgc cgcgcgccgt gaggccgagc aacagcgcct gcgcgccacc
     2581 gcgctgcttc atcacctgga acgcgcccgc gcgctgctcg cccgcccgag ccggccccgg
     2641 ccgcgggctg gcgtgccgtc gcagctaccg ctgaccctgc catcgaggac cgaccgatga
     2701 cttcagcaac aacccgccgc gctcgcgtcg atgccgtgct cgccgaactg cgcgagcggt
     2761 ttccggctgc gttcccgacc gacctgtcga ccgtccggcc gctggcgctg ggcatccgcg
     2821 aggccatcga ggcactgaag atcgccggcc gcacgccggt gcatcgcgtg ctctggctct
     2881 ggacccggac accggcct
//
LOCUS       DAOQIE010000412         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23525_length_2882_cov_2.860983, whole genome shotgun sequence.
ACCESSION   DAOQIE010000412 DAOQIE010000000
VERSION     DAOQIE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23525_length_2882_cov_2.860983"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="PK144_17595"
     CDS             <1..1897
                     /locus_tag="PK144_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67589.1"
                     /translation="GALICRRPPHNRAEEHDDNLEVRPLPGGGCRLAAADAGQRSFRI
                     AYRSADARVRALVWPVAGVFVDLLDYGVTPVRERPLASGELLAVRRDSRAVLDVRTGG
                     AGELRIGTHRYPVAAGGRRRLPVALLAEVLEADADTLQFVPAGSPTRQTVLRLVLQHR
                     TLAFTAGSPRGSKACGFRLAAPLQQLRLTALNLVSGRNQTLTLIPDDPVDCGAGPAWL
                     GSEPLTEGGWQYTLTLAPERWQTGAWVLTLAGELDGRPGWLLDAHGERCSVGLLVRST
                     GERTALAETLAVPAGWLDSHGFGLLARVQETLQTCPRAIHRPDLDWLPRLWRQLARQC
                     EPTDAQSLTRLLALSLRAPAGNDRDDDATDVPHFTLGSVLPTLYTRPAVEYAGFGDGA
                     FAALAATGRPPAEVFGQHLSPLAAAGFVNFATVVQGACPREFAFGRYARALAGAGDRR
                     AFRPDWQPHGADCLGPQHLPWARAALRARLTATQASADADARPGHALRLARCLQHQPA
                     GGWLPGLSSALLDFRPVLGTPAAVPDEQAGTEDTLDVLAHALALYAGACRLEARVPGT
                     LARGTRQLACCLPDTAPDQRQAALGWLLAAGRELFAFYLLLWELALSTDLADGVPAWS
                     AATTPQH"
     gene            1941..>2882
                     /locus_tag="PK144_17600"
     CDS             1941..>2882
                     /locus_tag="PK144_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF67590.1"
                     /translation="MSESNPRRLHDALNATLRRYIPTTLPISRRYPELRREVREQVAA
                     QTLVQGPYVEALPDFEKGRPLRALLRGNGGYLHDGFGALPAALLDRPLHRHQEQALEL
                     ACRDGDNLLVATGTGSGKTECFLYPLVQRLLDDPEPQRPGVRCLLIYPMNALANDQLY
                     YRIAPLFGHLLGAAGISFGRFTSQIRAHTRRFEEESRLRENDRLMTALGGAIPAHWRL
                     TREEMLAAPPKILITNYAMLEHLLLLPRNAPLFAQDALQCIVLDEIHTYSGAQATEVA
                     FLLRKLKNRLGLERRLQVFGTSASLPEGEAAEVCIRRF"
BASE COUNT          369 a         1088 c         1022 g          403 t
ORIGIN      
        1 cggcgcgctg atctgtcgcc gcccgccgca caaccgcgcc gaagaacacg acgacaacct
       61 cgaagtccgg ccgctgcccg gcggcggctg ccggctggcc gccgccgatg ccgggcagcg
      121 cagcttccgc atcgcgtacc gcagcgccga tgcgcgggtg cgcgcgctgg tctggccggt
      181 cgccggggtg ttcgtggacc tgctcgacta cggcgtcacg cccgtacgcg aacggccgct
      241 ggcaagcggc gagctgctgg cggtgcggcg cgactcgcgc gccgtgctcg atgtgcggac
      301 cggcggcgcc ggtgagctgc gcatcggcac gcatcgctat ccggtcgcgg ccggcggccg
      361 gcgccggctg ccggtggcgc tgctcgccga ggtgctggag gcggatgccg acacgctgca
      421 gttcgtgccg gccggcagcc cgacgcgcca gacggtgctg cggctggtgc tgcagcaccg
      481 cacgctggcg ttcacggccg gctcgccgcg cggcagcaaa gcctgcggct tccggctcgc
      541 cgcgccgctg cagcagttgc ggctcacggc gctgaacctg gtcagcggcc gcaatcagac
      601 gctgacgctg atccccgacg atccggtcga ctgcggcgcg ggccctgcct ggctcggcag
      661 cgagccgctg accgaagggg gctggcagta cacgctgacg ctggcgccgg agcgctggca
      721 gaccggggcc tgggtgctga cgctggcggg cgaactcgat ggtcgccccg gctggctgct
      781 cgatgcccac ggcgagcgct gcagcgtcgg gctgctcgtc cgtagcaccg gcgaacgcac
      841 ggcgctggct gaaacgctgg cggtaccggc cggatggctc gattcccacg gtttcgggct
      901 gctggcccgc gtgcaggaga ccctgcagac ctgcccgcgc gccattcacc ggcccgacct
      961 cgactggctg ccgcggctgt ggaggcaact cgcccgccag tgcgaaccga ccgatgcgca
     1021 gtcgctgacc aggctgctgg cactgagcct gcgcgccccg gccggaaacg accgggacga
     1081 cgacgccacc gatgtgccgc atttcacgct cggcagcgtg ctgccgacgc tgtacacccg
     1141 gccggccgtc gaatacgccg gtttcggcga cggtgcgttc gcggcgctgg ccgccaccgg
     1201 ccggccgccg gccgaggtgt tcggccagca cctgtcgccg cttgccgctg ccggtttcgt
     1261 caatttcgcc acggtcgtgc agggcgcctg cccgcgggaa ttcgcgttcg ggcgctatgc
     1321 ccgggcactg gccggagccg gtgaccggcg ggccttccgc cccgactggc agccgcacgg
     1381 cgccgactgc ctcggcccgc agcacctgcc gtgggcgcgc gcggcgctgc gggcacggct
     1441 gacggcaacg caggcgagtg ccgacgccga cgcccggccc ggccatgcgc tgcgcctcgc
     1501 ccgctgtctg cagcaccagc cggccggcgg ctggctgccg gggctgtcga gcgcgctgct
     1561 cgatttcagg ccggtgctcg gcacaccggc tgccgtcccg gacgagcagg cgggcaccga
     1621 ggacaccctc gatgtcctcg cgcacgcgct ggcgctgtac gccggcgcct gccggctgga
     1681 ggcacgcgtg cccggcacgc tggcgcgcgg cacgcggcag ctcgcctgct gcctgccgga
     1741 taccgcaccc gaccagcgtc aggccgccct cggctggctg ctcgccgccg gtcgcgagct
     1801 gttcgcgttc tacctgctgc tgtgggaact ggcgctgtcg accgatctgg ccgacggcgt
     1861 gcccgcctgg tcggcggcga ccaccccgca gcactgatcc cggccacggc tggacacttc
     1921 ctctgttcgg agcctgcccg atgagcgaat ccaatccgcg tcgcctgcac gatgcgctga
     1981 acgcgacgct gcgccgctac atcccgacca cgctgccgat cagccgccgc taccccgagc
     2041 tgcgccgcga ggtgcgcgag caggtcgcag cgcagacgct ggtgcagggg ccgtacgtcg
     2101 aggcgctgcc ggacttcgag aagggccggc cgctgcgggc gctgctgcgc ggcaacggcg
     2161 gctatctgca cgacggcttc ggcgcgctgc cggccgccct gctcgaccgg ccgctgcacc
     2221 ggcatcagga acaggcgctg gagctggcct gccgcgacgg cgacaacctg ctggtcgcca
     2281 ccggcaccgg ttcgggcaag accgaatgct ttctgtatcc gctcgtgcag cgcctgctcg
     2341 atgaccccga gccgcagcgc ccgggcgtgc gctgcctgct gatctacccg atgaatgcgc
     2401 tggccaacga ccagctctac taccgcatcg cgccgctgtt cgggcatctg ctcggtgcgg
     2461 ccggcatcag cttcggccgc ttcacgtccc agatccgcgc ccatacccgc cgcttcgaag
     2521 aggaaagccg gctgcgcgag aacgaccggc tgatgaccgc cctcggcggt gcaatcccgg
     2581 cgcactggcg gctgacgcgc gaggaaatgc tcgccgcgcc gccgaagatc ctgatcacca
     2641 actacgcgat gctcgaacac ctgctgctgc tgccgcgcaa cgcaccgctg ttcgcacagg
     2701 acgcgctgca gtgcatcgtg ctcgacgaga tccataccta cagcggtgcg caggcgaccg
     2761 aggtcgcctt cctgctgcgc aagctcaaga accggctcgg cctcgagcgc cggctgcagg
     2821 tgttcggcac cagcgccagc ctgccggaag gcgaggctgc cgaagtctgc atccggcgtt
     2881 tc
//
LOCUS       DAOQIE010000413         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_23825_length_2858_cov_6.417767, whole genome shotgun sequence.
ACCESSION   DAOQIE010000413 DAOQIE010000000
VERSION     DAOQIE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23825_length_2858_cov_6.417767"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK144_17605"
     CDS             <1..183
                     /locus_tag="PK144_17605"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF67591.1"
                     /translation="DELDFGPGTTTLMTGKDAVKCRGFPGNQARWWLAVQAVTEPDLA
                     AALAARLPPPRSAAET"
     gene            187..366
                     /locus_tag="PK144_17610"
     CDS             187..366
                     /locus_tag="PK144_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNF67592.1"
                     /translation="MDKKLLDILVCPVSKAPLVYDKERQELKCKASRLAYPVRDGIPV
                     MLEAEARRMADDEPV"
     gene            373..1143
                     /gene="kdsB"
                     /locus_tag="PK144_17615"
     CDS             373..1143
                     /gene="kdsB"
                     /locus_tag="PK144_17615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNF67593.1"
                     /translation="MTDATVDFRVAIPARYASTRLPGKPLLAIAGRPMIEHVWRLACA
                     SGAREVVIATDDERIRTAAAGFGAEVVMTRADHASGTDRLAEVAEQRGWPDEAIVVNV
                     QGDEPLLPPALVRQTAEALAADALAGIATLAAPLAAHELFDPNAVKVVCAADGRALYF
                     SRATIPWDRDRFARGEREPDPALPYWRHIGLYAYRAGVLRAYPRLAPSPLERAETLEQ
                     LRALWHGIAIRVLFTDPAPPAGVDTAEDLRRAEAALGG"
     gene            complement(1140..1409)
                     /locus_tag="PK144_17620"
     CDS             complement(1140..1409)
                     /locus_tag="PK144_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67594.1"
                     /translation="MPLPDTARLTGLIGRELDWLGQRCRIVELLPEGPSLVLEAVGAE
                     PSIQANQYGEATRRAVETHTVALLSPRGDALNPLLPGLPALLAAL"
     gene            1439..1999
                     /locus_tag="PK144_17625"
     CDS             1439..1999
                     /locus_tag="PK144_17625"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNF67595.1"
                     /translation="MGLWQRLGSVFKNDRQASQPLRRDPKVAVRVLMVCLGNLCRSPT
                     AEAVLAHLAVQAGLGRVIEIDSCGLSASNRGEPPDRRAREAAARRGYSMDDLRTRRFD
                     RDDLNYFEYVIAMDREVLETLQGYCMTPVQDAKLHLLLDYAPQLPEREVPDPYFGGRS
                     GFERVLDLIEPAATGLLAHLRREYRL"
     gene            complement(2316..>2858)
                     /locus_tag="PK144_17630"
     CDS             complement(2316..>2858)
                     /locus_tag="PK144_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67596.1"
                     /translation="ADILGEAQVAAGAIADMPAEAPETPAEVMGDVEAGDTVVEAAVA
                     VTEAPVPEMAGATDTPAIEAAPDGQPEVVVPPLPVDAPQPVLPEAPVTTPEVLAGTEA
                     AATTVTDADDASASGEAPAETPVQTPEAAAAAEAPASPGPAAEPELVEPPVTPPEALA
                     ATETGTAGSDGEPTPPQAAR"
BASE COUNT          399 a         1047 c         1007 g          405 t
ORIGIN      
        1 gacgaactcg acttcggccc tgggaccacg acgctgatga ccggcaagga tgccgtaaaa
       61 tgccgcggct tcccgggcaa ccaagcgcgc tggtggctgg ccgtgcaagc cgtcaccgaa
      121 cccgatctgg ccgcggccct ggccgcgcgc ctgcccccgc cccgctccgc cgccgagacc
      181 tgagccatgg acaagaaact cctcgacatc ctcgtctgcc ccgtcagcaa ggcgccgctg
      241 gtctatgaca aggagcgtca ggaactcaag tgcaaggcga gccgcctcgc ctacccggtg
      301 cgcgacggca tcccggtcat gctcgaagcc gaagcccggc gcatggccga cgacgaaccg
      361 gtctgaacgc cgatgaccga tgccacggtt gatttccgcg tcgcgatccc ggcgcgctac
      421 gcctcgaccc gcctgcccgg caagccgctg ctcgcgatcg ccggccggcc gatgatcgag
      481 cacgtctggc ggctggcctg cgcgagcggc gcccgcgagg tcgtcatcgc caccgacgac
      541 gaacgcatcc gtaccgcggc cgcgggcttc ggcgccgagg tggtgatgac gcgcgccgat
      601 cacgcctccg gcaccgaccg cctcgccgaa gttgcagaac aacgcggctg gccggacgag
      661 gcgatcgtcg tcaacgtcca gggcgacgaa ccgctgctgc cgccggcact ggtgcgccag
      721 accgccgaag cactcgccgc cgacgcgctc gccggcatcg cgacgctcgc cgcgccgctc
      781 gccgcgcacg aactgttcga tccgaacgcc gtgaaggtgg tctgcgccgc cgatggccgg
      841 gcgctgtact tcagccgcgc cacgatcccc tgggaccgcg accgcttcgc gcgcggtgaa
      901 cgcgagccgg acccggcgct gccgtactgg cggcacatcg gcctgtatgc gtatcgcgcc
      961 ggggtgctgc gcgcgtatcc gcgactggca ccgtcgccgc tggagcgcgc cgaaacgctg
     1021 gagcagctgc gggcgctgtg gcacggcatc gcgatccgcg tgctgttcac cgacccggca
     1081 ccgccggccg gcgtcgacac ggccgaggac ctgcgccgtg ccgaagccgc gctcggcggc
     1141 tagagcgcgg cgagcagcgc cggcaggccg ggcagcagcg ggttcagcgc atcgccgcgc
     1201 ggactgagca gcgctaccgt atgggtctcg acggcgcggc gcgtggcctc gccatactgg
     1261 ttggcctgga tcgacggctc ggcgccgacg gcttcgagca ccagcgacgg accttcgggc
     1321 agcagctcga cgatccggca acgctggccg agccagtcga gttcgcggcc gatcaggccg
     1381 gtgagtcggg cagtatcggg caggggcatc ggaggtgcct ggcggcagga ggacggtgat
     1441 gggattgtgg caacgcctcg gcagtgtgtt caagaacgac cgtcaggcct cgcagccgct
     1501 gcggcgcgat ccgaaggtcg cggtgcgcgt gctgatggtc tgtctcggca atctgtgccg
     1561 ctcgccgacc gccgaggccg tgctcgcgca cctcgccgtg caggccgggc tcggccgggt
     1621 catcgagatc gactcctgcg gactgtcggc cagcaaccgc ggcgaaccac cggaccggcg
     1681 cgcgcgcgag gccgccgccc ggcgcggcta ctcgatggac gacctgcgca cgcgccgctt
     1741 cgatcgcgac gacctgaact acttcgaata cgtgatcgcg atggaccgcg aggtgctgga
     1801 aaccctgcag ggctactgca tgacgccggt ccaggacgcg aagctgcact tgctgctcga
     1861 ctacgccccg caactgcccg agcgcgaggt gccggacccg tacttcggcg gacgcagcgg
     1921 cttcgagcgc gtgctcgacc tgatcgaacc ggccgcaacc ggcctgctcg cccacctgcg
     1981 ccgcgaatac cggctctgag tccggtcatg ccgcggccga gcgaaagcgg tgccggtgcc
     2041 ggctttgcgt gtcgtgcctt gccgaggcat gtcacggaat ccgaggaatc catggatttg
     2101 cccgggccgg cagcacgcag gatgcgctga ggaacgaagc acatcgttcg cgcaaagcgc
     2161 tggaaggcac gggaatctcg atctcggccc gaatttcgcg agcggtgcgc ctcctgcgcc
     2221 ggcacatcct gcggctgggc cgcgcagaca gcacgaaggg gcgccggaaa gtccggcgcc
     2281 ccttctttgc tgctggcgct ccgcgggctg cggcgtcagc gcgccgcctg cggcggggtc
     2341 ggctcgccgt cgctgccggc cgtgccggtt tcggtcgcag ccagcgcttc cggcggcgtg
     2401 accggcggct cgacaagctc aggctctgct gccggtccgg ggcttgccgg ggcctcggcc
     2461 gctgcagccg cttcgggtgt ctgaaccggc gtttcagccg gcgcttcgcc cgacgcgctg
     2521 gcgtcgtccg cgtccgtcac cgtcgttgcg gccgcttcgg taccggccag cacctccggc
     2581 gtcgtgaccg gtgcctcggg cagcacgggc tgcggcgcat cgaccggcag cggcggcacg
     2641 acgacttcag gctgcccgtc cggcgcggcc tcgatggccg gcgtgtccgt cgccccggcc
     2701 atttccggga ccggcgcttc ggttacggct acggcagctt cgaccaccgt gtcgccggcc
     2761 tcgacatcgc ccatgacctc ggctggcgtc tccggcgctt cggccggcat atcggcgatg
     2821 gcaccggcag cgacctgcgc ctcgccgagg atgtcggc
//
LOCUS       DAOQIE010000414         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_24220_length_2830_cov_6.118919, whole genome shotgun sequence.
ACCESSION   DAOQIE010000414 DAOQIE010000000
VERSION     DAOQIE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24220_length_2830_cov_6.118919"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1145
                     /locus_tag="PK144_17635"
     CDS             165..1145
                     /locus_tag="PK144_17635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNF67597.1"
                     /translation="MSISDQPCEDFESAVRAVLGYLHARFGFDLWMVTRTEGEDWIVL
                     HVEDHGYGVRPGTVFRWSDSFCSRMVQGLGPRVAPDAAGVPAYAEAPIGRQVRIGAYI
                     GLPLRRADGSLFGTLCAIDPRPQDESLRAEQPLLELLALLLGTVLQKDLAATEAARRA
                     ERLQAEALTDAMTALYNRRAWTRFLEHEEGRCRRYGAAACVIVVDLDGLKRINDGSGH
                     AAGDELICRAAGALRKAARTVDVVARLGGDEFGVLCAECDGPHGRRLAGRLRTALREA
                     GVDASIGIACRRPDAGIQAAWEQADQQMYEEKKTRTGGGRDQPAGGRFAS"
     gene            complement(1115..1741)
                     /gene="mobA"
                     /locus_tag="PK144_17640"
     CDS             complement(1115..1741)
                     /gene="mobA"
                     /locus_tag="PK144_17640"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNF67598.1"
                     /translation="MSEGIRDGVACSAGISAVILAGGRGSRMGGVDKGLIEVAGTALI
                     DRTLTALRPQVSTVLINANRNREAYARRGCPVLADATPDYDGPLAGMAAALAVIETPW
                     LLAVPCDCPQLAPDLAARLLAAAEAAGAELAVAHDGERLQPVFVLLHRDLRVPLAQYL
                     DGGGRKLEHWYRSRRMTEADFSDRPQTFVNLNSPDEKSAYEANRPPAG"
     gene            1936..>2830
                     /locus_tag="PK144_17645"
     CDS             1936..>2830
                     /locus_tag="PK144_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF67599.1"
                     /translation="MSFCPVPAVPEPLPRLDALAVLVVDDEPGMCNFLCRALARHVAR
                     VEAAGSLEAARAARLAAHYDLLLVDQRLPDGSGVDWLAELRAAGAHEQLIVITAYADL
                     DTAIRALRAGAADFLLKPARLDQVLESVRRAAALLPQPGRPQPPAPVAELIGDSTAIR
                     DLSALIRRIAPHPSTLLIEGESGTGKECVARAVHRLSGRSGPYVPLNCGALPRELLES
                     ELFGHVRGAFSGALAGREGLFAHADGGTLFLDEISELPLGMQTALLRVLEDRRVRPLG
                     SERERAIDVRIITASNRDLAAE"
BASE COUNT          374 a          986 c         1047 g          423 t
ORIGIN      
        1 cgtaatgaat tacgggatcg cgaatgcagg gccgaaagcc ggccattgcc ggcactcgat
       61 gccggctgcg gaatggcgca ggtgccggcg ctgtcacagg atcggggcgt gttccggcag
      121 gcatgccgcc cgcatccccc ggcagccggt gtgcaggtgc tcccatgtcg atcagcgacc
      181 agccttgcga agacttcgag agtgcggtcc gggcggtgct cggctatctg catgcacgct
      241 tcgggttcga tctctggatg gtgacgcgca ccgaaggcga ggactggatc gtgctgcacg
      301 tcgaggacca cggctacggc gtgcgccccg gcacggtgtt ccgctggtcg gattccttct
      361 gttcgcggat ggtgcaggga ctcgggccgc gggtggcgcc cgatgccgct ggcgtgccgg
      421 cctatgccga ggcgccgatc ggccggcagg tgcgcatcgg tgcctacatc ggcctgccgc
      481 tgcgccgggc cgatggctcg ctgttcggca cgctgtgtgc gatcgatccg cggccgcagg
      541 atgaatccct gcgcgccgaa cagccgctgc tcgaactgct ggcgctgctg ctcggcaccg
      601 tgctgcagaa ggatctggcc gcgaccgagg cggcgcgccg tgccgagcgc ctgcaggccg
      661 aggcgctgac cgatgcgatg acggcgctgt acaaccggcg cgcctggacc cgcttcctcg
      721 aacacgagga aggccgctgc cggcgctacg gtgccgctgc ctgcgtgatc gtcgtcgacc
      781 tcgatggcct caagcgcatc aacgacggtt cggggcacgc cgccggcgac gagctgatct
      841 gccgggccgc cggggcgctg cgcaaggccg cgcgcacggt cgatgtggtc gcgcgcctcg
      901 gcggcgacga attcggcgtg ctgtgcgccg agtgcgatgg gccgcacggc cggcggctcg
      961 ccggacggct gcgcacggcg ctgcgcgaag ccggggtcga tgcctcgatc ggcatcgcct
     1021 gccgccggcc cgacgccggc atccaggccg cctgggaaca ggccgatcag cagatgtacg
     1081 aggagaagaa gacccgcacg ggcggcggac gcgatcagcc ggccggcggc cggttcgcct
     1141 cgtaggcgga tttctcgtcg ggtgagttga ggttcacgaa ggtctgcggc cggtcgctga
     1201 aatcggcttc ggtcatgcgc cggctccggt accagtgctc aagcttgcgc ccgccgccgt
     1261 ccaggtattg cgcgagcggc acgcgcaggt cgcggtgcag cagcacgaac accggctgca
     1321 ggcgctcgcc gtcgtgcgcg accgcgagtt cggcaccggc ggcttcggcg gcggcgagca
     1381 ggcgggcggc cagatccggc gcaagctgcg ggcagtcaca gggcacggcc agcagccacg
     1441 gcgtttcgat caccgccagc gccgcggcca tgccggcgag cgggccgtcg tagtccggcg
     1501 tggcatcggc gagcaccggg cagccgcgcc gcgcataggc ttcccggttg cggttggcgt
     1561 tgatcagcac cgtggacacc tgcgggcgca gcgcggtgag cgtgcggtcg atcagcgccg
     1621 ttccggccac ctcgatcagg cccttgtcga ccccgcccat gcggctgccg cggccgccgg
     1681 cgaggatcac cgcgctgatg ccggcactgc aggcgacgcc atcacggatg ccctcactca
     1741 tgtctgcctc tcatgctcgg tcccgcgcat gtctgcggat tccggctgcc ggtgaaggag
     1801 ggggcagcat agcgactgct gcgcgctgcg cacggacgct gcttggtgcg gtgcagcgtg
     1861 aattcgggcc tgcatggccc atacattgcc ggcaggtaat gcatgtttca ttcacgtcca
     1921 ctgccggtga cgctcatgtc cttctgcccc gtgcccgccg ttccggagcc gttgccgcgc
     1981 ctcgatgcac tggcggtgct cgtcgtcgat gacgagcccg gcatgtgcaa cttcctctgc
     2041 cgggcgctgg cacgccatgt cgcgcgggtc gaagcggccg gcagcctgga ggcggcgcgt
     2101 gcggcgcgcc tggcggcgca ctacgacctg ctgctggtcg accagcgcct gccggacggt
     2161 tcgggcgtcg actggctggc agaactgcgc gccgccggcg cgcacgagca actgatcgtg
     2221 atcacggcct acgccgatct cgacaccgcg atccgggcgc tgcgcgccgg tgccgccgac
     2281 ttcctgctga agccggcgcg gctcgatcag gtgctcgaat ccgtgcgtcg cgccgcggcg
     2341 ctgctgccgc agccgggccg gccgcagccg ccggcaccgg tcgcggagct gatcggcgac
     2401 agcacggcga tccgtgatct gagcgcgctg atccggcgca tcgcgccgca tccctcgacg
     2461 ctgctgatcg agggcgagag cggcaccggc aaggaatgcg tcgcgcgcgc cgtgcatcgc
     2521 ctgagcgggc gcagcggccc ctacgtaccg ctcaactgcg gcgcgctgcc gcgcgagctg
     2581 ctggagagcg agctgttcgg acacgtgcgc ggtgccttca gcggcgcgct ggccgggcgc
     2641 gagggcctgt tcgcgcacgc cgacggcggc acgctgttcc tcgacgagat cagcgagctg
     2701 ccgctcggca tgcagacggc gctgctgcgc gtgctggagg accggcgcgt gcgcccgctc
     2761 ggcagcgagc gcgagcgcgc gatcgacgtg cgcatcatca ccgccagcaa ccgcgatctg
     2821 gccgccgagg
//
LOCUS       DAOQIE010000415         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_24292_length_2825_cov_7.892058, whole genome shotgun sequence.
ACCESSION   DAOQIE010000415 DAOQIE010000000
VERSION     DAOQIE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24292_length_2825_cov_7.892058"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1406)
                     /locus_tag="PK144_17650"
     CDS             complement(21..1406)
                     /locus_tag="PK144_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNF67600.1"
                     /translation="MLGILGIVLSLALLIFLAYRGWNVIFIAPFCATVALLFNWGDAP
                     LLATYTQTFMPALGNYFAKFFPLFLLGAVFGKLMDDSGSARAIAHKIVQLVGRERSML
                     AVVLACAILTYGGVSLFVVAFSVYPIAVSLFRESGIPKRLIPAAIGLGSFTFTMTALP
                     GTPAIQNAIPMPFFGTNTFAAPGLGIIAGVFMFAFGQFWLNRRVAQARARGEGYGNHE
                     DALSQAAEQGMPPFWLALLPIVVVIVLNYIATSYVIPAMDTAYLATDKFKKTPLSAVL
                     GIWSIILAMLVAITLVAVLNRKRIANLLDTFNKGTMGSLLPMANVASEVAYGAVIASL
                     AAFAIVREGLKSVASDPVTSLFIVVTVLAGMTGSASGGMSLALGAMGEYYAHAAQAAG
                     INPEIMHRIAALGSAAMDILPHNGAVITLLAICSLTHRESYLDLFMVGSLSALLSSVL
                     VLGLNAVFGTF"
     gene            complement(1745..2485)
                     /gene="phbB"
                     /locus_tag="PK144_17655"
     CDS             complement(1745..2485)
                     /gene="phbB"
                     /locus_tag="PK144_17655"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNF67601.1"
                     /translation="MGKVAIVTGGIGGLGTAMCKALCAQGRTVVAAYYPAEADKAAEW
                     QKARKEEGLDIAVYPVDVSDLESCAQLVAKVEADLGPVEILVNNAGITRDSVFKRMTD
                     AQWLAVINTNLNSLFNMTKPVFEKMTSRGWGRIINISSVNGQKGQFGQANYSAAKAGA
                     HGFTMALAQEGAKAGVTVNTISPGYIGTDMVMAIAEEVRNKIIATIPVGRLGTPEDIA
                     RTVAFLADDEAGFITGADMYVNGGLYIH"
BASE COUNT          509 a          902 c          948 g          466 t
ORIGIN      
        1 tgtctccgcc tccgtccgcc tcagaaggtg ccgaacacgg cgttcagccc gagcaccagc
       61 acgctcgaca gcagcgccga cagcgatccg accatgaaca ggtcgaggta cgactcccgg
      121 tgcgtcagcg agcagatggc gagcagcgtg atgacggcgc cgttgtgcgg caggatgtcc
      181 atcgccgccg agccgagggc cgcgatgcgg tgcatgatct cggggttgat gccggccgcc
      241 tgtgccgcat gagcgtagta ctcacccatc gccccgagcg cgagactcat gccgccggag
      301 gccgaaccgg tcatgccggc gagcaccgtg accacgatga acagcgaggt caccggatcg
      361 gaggcgaccg acttcaggcc ctcgcgcacg atcgcgaacg cggccaggct cgcgatcacc
      421 gcgccgtagg cgacttccga ggcgacgttg gccatcggca gcagcgaacc catcgtgccc
      481 ttgttgaagg tgtcgagcag gttggcgatg cgcttgcggt tgagcacggc caccagggtg
      541 atcgccacca gcatcgcgag gatgatcgac cagatgccga gcacggccga cagcggcgtc
      601 ttcttgaact tgtcggtcgc cagatacgcg gtgtccatcg ccgggatcac atagctggtg
      661 gcgatgtagt tcagcacgat gacgacgacg atcggcagca gcgccagcca gaacggcggc
      721 atgccctgct cggcagcctg actcagcgca tcctcgtggt tgccgtagcc ttcgccgcgg
      781 gcgcgggcct gggcgacgcg gcggttcagc cagaactggc cgaacgcgaa catgaacacg
      841 ccggcgatga tgccgaggcc gggcgcggcg aaggtgttgg tgccgaagaa cggcatcggg
      901 atggcgttct ggatcgccgg cgtgccgggc agcgccgtca tcgtgaacgt gaacgagccg
      961 aggccgatcg ccgccgggat caggcgcttg ggaatgccgg actcccggaa cagcgacacc
     1021 gcgatcggat acaccgagaa ggcgacgacg aacagcgaca caccgccgta ggtcaggatc
     1081 gcgcaggcca gcaccaccgc gagcatcgag cgctcgcgcc cgaccagctg cacgatcttg
     1141 tgcgcgatcg cgcgggccga gcccgaatca tccatcagct tgccgaacac cgcgccgagc
     1201 aggaacagcg ggaagaactt ggcgaagtag ttgccgagcg ccggcatgaa ggtctgggtg
     1261 taggtcgcga gcagcggcgc gtcaccccag ttgaacagca gcgccacggt ggcacagaac
     1321 ggcgcgatga agatgacgtt ccagccgcgg tacgcgagaa agatcagcaa ggccagggac
     1381 agcacgatgc caagtatgcc gagcacggtg ggctccttcg gtggtggacg aatgccccct
     1441 ttgcacccgg gggctgactt ccatgagcca agcaccgtgc cagccggcaa ttaaaataca
     1501 acttattgtt ttttaatgaa ttaaagaaca gacctgcatg cttgccgcca gccctccgtc
     1561 cggatttccg gacacgtccg atacgaccgc ggccgtatcg gcgtggattt gtgcgggcgc
     1621 ggctgaattt ttttgaattt ccgcgggcgt gccggcgccg tccggaaaaa aagacaccgt
     1681 ccgggaatcc ggacggtgtc ggggtctgac gcttgcggcg ctgcggcgcc ggccggtcgg
     1741 acgatcagtg gatgtacaga ccgccgttga cgtacatgtc ggcgccggtg atgaagccgg
     1801 cttcgtcatc ggccaggaag gcgaccgtgc gcgcgatgtc ttccggcgtg ccgagacggc
     1861 cgaccgggat cgtcgcgatg atcttgttgc ggacttcctc ggcgatggcc atgaccatgt
     1921 cggtgccgat gtagcccggc gagatggtgt tgacggtcac gccggccttg gcgccttcct
     1981 gcgccagcgc catcgtgaag ccgtgcgcac cggccttggc ggccgagtag ttggcctggc
     2041 cgaactggcc cttctggccg ttgaccgacg agatgttgat gatccggccc cagccgcgcg
     2101 aggtcatctt ctcgaacacc ggcttggtca tgttgaacag gctgttcagg ttcgtgttga
     2161 tgacggccag ccactgggcg tcggtcatgc gcttgaacac ggaatcgcgc gtgatgccgg
     2221 cgttgttgac caggatctcg accggaccga ggtcggcctc gaccttggcg acgagctgcg
     2281 cgcagctttc gaggtcgctc acgtccaccg gatagacggc gatgtccaga ccttcttcct
     2341 tgcgggcctt ctgccattcc gcagccttgt cggcctcggc ggggtagtag gccgccacga
     2401 cggtacggcc ctgggcgcac agcgccttgc acatcgcggt tcccaggcca ccgatgccgc
     2461 ccgtaacgat tgccaccttg cccattgcat cccccgtaga ctgttcatga tgcggtgtac
     2521 cagcgggggc cggtcaccgc gggacgaagt gtcaccacaa tggtgctgcg tcgcaccatc
     2581 gtggcgcccg atcccccgtc agggccgcat ttacaagcag atgcgcggcc accggcggga
     2641 aggcgcgtat gctggagcaa gacgcacgcc attggaacgg tgattggaac ggtaacgtgc
     2701 aaattccttg cgtcgccccg ctttggcgca gccgccgccg cggattcagc ccgtgccctg
     2761 caggccgagc agcgcgagct tttcgtacag cgtcgcgcgc gagatgccga gcagcttcgc
     2821 cgcat
//
LOCUS       DAOQIE010000416         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_24574_length_2805_cov_4.574545, whole genome shotgun sequence.
ACCESSION   DAOQIE010000416 DAOQIE010000000
VERSION     DAOQIE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24574_length_2805_cov_4.574545"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="PK144_17660"
     CDS             complement(<1..1230)
                     /locus_tag="PK144_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HNF67602.1"
                     /translation="MPLPLPNLDDRRFDDLVAELRARLASHVPEWELAPGDPGWALID
                     VLAWLAETILYRANLIPERQRRAFLDLLGLPLRAAAPARGVVCIDSAPNAPQPVPLLP
                     AGSRLRAGAVTCTTETELQPSALELLAVQKARLDAATLGARGVSLDELRALYNRPVTP
                     FEPRALDPATGADFASALDGALWLALLRPRNAPQDAAALRAAFAGRTLSLGLAPDEEA
                     PDGLVARALPARSLKLELVHARSGAEPGWLALEILDDRSLGARQPGVLRFRLPANVLL
                     APPLYDDPKDAGLGVRPPALPAGRSGDELVHWLRLSCPDAPGLKLAWLGFNAVDVVAL
                     DQRRDVLLAVGDGRPDQVCALPDGNVDAASLLLEVETPQTTAAGWERWSPVERFAAAG
                     REAPVYALDAASGRIRFG"
     gene            complement(1246..1677)
                     /locus_tag="PK144_17665"
     CDS             complement(1246..1677)
                     /locus_tag="PK144_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNF67603.1"
                     /translation="MDRQHDGPVRAARGWPLGGLPDPADAGAGATAGQLAWTSGERAI
                     RESLINILLTRPGERLQRPDFGAGVRDWIHRPNDATSRGLLAAVIERALRRDEPRIAL
                     EAVEVVAPPDDPTQIGITIRYRILASGQRDGLALTLAPERP"
     gene            complement(1677..>2805)
                     /locus_tag="PK144_17670"
     CDS             complement(1677..>2805)
                     /locus_tag="PK144_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67604.1"
                     /translation="QRRLVAAQRLGAAALKAPYDGEATLPLHAQLVRDAGGRAHCVLW
                     GYVGLGGSAPGRISAPAPADDPAVLELASEIPWPLGGAERDWLAGDGALLVNGAAHPA
                     LTGLLRVLLLRYRLGADGAAAQPRLAELAAALVLKTTLATDFQRNGRPVSLLDWLRED
                     NDALQDWLNTGTPSTYPPALTGGGGGLNLTLAADDARQWRLALASARDGLLAGYRDAV
                     PIPRYGQGANEVFRVHPFVRTLDACGCERITFGAPSLAFRVAAPWEPEAARPVAIPVP
                     GLADLRRGLARGVSFRMPADTGSLIAGLRGKDRAKDALDGNASQGAGFGFGMLCSFSL
                     PILTLCALILLMVIVALLNFVFRWLPFVYLCIPWPKREGDG"
BASE COUNT          371 a         1019 c         1102 g          313 t
ORIGIN      
        1 gccgaagcgg atgcggccgc tcgcggcatc gagtgcgtag acgggcgcct cgcgcccggc
       61 agcggcgaag cgctcgaccg gcgaccagcg ctcccagccg gccgcggtgg tctgcggcgt
      121 ctcgacttcg aggagcagcg atgcggcatc gacgttgcca tcgggcagtg cgcagacctg
      181 atccggccgg ccgtcgccga cggcgagcag cacgtcgcgg cgctggtcga gcgcgacgac
      241 atcgacggcg ttgaagccga gccacgcgag cttcaggccc ggcgcgtcgg ggcaggacag
      301 gcgcagccag tgcacgagtt cgtcgccgct gcgcccggcc ggcagcgccg gcggacgcac
      361 gccgaggccg gcatccttcg gatcgtcgta gagcggcggc gcgagcagca cgttggccgg
      421 cagccggaag cgcagcacgc cgggctggcg ggcgccgagc gagcggtcat cgaggatttc
      481 cagcgccagc cagccgggtt cggcgccgct gcgcgcatgc acgagttcga gcttcaggct
      541 gcgggccggc agcgcgcgcg cgaccagccc gtcgggtgct tcctcgtccg gcgcgaggcc
      601 gagcgacagc gtgcggccgg cgaaggccgc gcgcagcgcc gcggcgtcct gcggcgcgtt
      661 gcgcgggcgc agcagcgcca gccacagcgc gccgtcgagg gcggacgcga agtcggcacc
      721 ggtggccgga tcgagcgcgc gcggctcgaa cggcgtcacc ggccggttgt agagcgcgcg
      781 cagttcatcg agcgacacgc cgcgcgcgcc gagcgtcgcg gcatcgagcc gcgccttctg
      841 taccgccagc agttcgagcg cgctcggctg cagctcggtt tcggtcgtgc aggtgacggc
      901 gccggcgcgc agccggctgc cggccggcag cagcggcacc ggctgcggcg cgttcggcgc
      961 gctgtcgatg cagacgacgc cgcgcgccgg ggccgcggcg cgcagcggca ggccgagcag
     1021 gtcgaggaag gcacggcgct ggcgctccgg gatcaggttg gcgcggtaca ggatcgtctc
     1081 ggcgagccag gccagcacgt cgatcagcgc ccagcccgga tcgccgggcg cgagttccca
     1141 ttcgggcacg tgactcgcga ggcgcgcgcg cagctcggcg accagatcgt cgaagcggcg
     1201 gtcgtcgagg ttcggcagcg gcagcggcat ggcggctccg gacggtcagg ggcgttcggg
     1261 ggccagcgtc agcgcgaggc cgtcgcgctg gccgctggcg aggatgcggt agcggatcgt
     1321 gatgccgatc tgcgtcgggt catcgggcgg ggcgacgacc tcgacggctt cgagcgcgat
     1381 gcgcggctcg tcgcgccgca gggcccgctc gatgacggcg gcgagcaggc cgcggctggt
     1441 ggcgtcgttc gggcgatgga tccagtcgcg cacgccggcg ccgaagtccg ggcgctgcag
     1501 gcgctcgccg ggccgggtca gcaggatgtt gatcaggctc tcgcggatcg cgcgctcgcc
     1561 gctcgtccac gcgagctgcc cggccgtcgc gccggcgccg gcatcggccg gatcgggcag
     1621 gccgccgagc ggccagccgc gggctgcgcg caccgggcca tcgtgttgcc ggtccatcag
     1681 ccgtccccct cgcgcttcgg ccacgggatg cacagataaa cgaagggcag ccagcggaag
     1741 acgaaattca gcagcgcgac gatgaccatc agcaggatca gcgcgcacag cgtcaggatc
     1801 ggcaggctga acgagcagag catgccgaag ccgaaaccgg cgccctgact ggcgttgccg
     1861 tcgagcgcgt ccttcgcgcg gtccttgccg cgcaggccgg cgatcaggct gccggtgtcg
     1921 gccggcatcc ggaagctgac gccgcgcgcc agaccccggc gcaggtcggc aagccccggc
     1981 accgggatcg cgaccggccg cgcggcctcc ggctcccacg gcgccgcgac ccggaacgcc
     2041 agcgacggtg cgccgaaggt gatgcgctcg cagccgcagg catcgagcgt gcgcacgaag
     2101 ggatgcacgc gaaacacctc gttcgcgccc tggccgtagc gcgggatcgg caccgcgtcg
     2161 cgatagccgg cgagcaggcc gtcgcgcgcc gaggcgagcg cgagccgcca ctggcgggcg
     2221 tcgtcggcgg ccagcgtcag gttcaggccg ccgccgccgc cggtgagcgc cggcgggtac
     2281 gtcgacggcg tgccggtgtt cagccagtcc tgcagcgcat cattgtcctc gcgcagccag
     2341 tcgagcaggc tgaccggccg gccgttgcgc tggaagtcag tcgccagcgt cgttttcagc
     2401 accagcgccg cggcgagttc ggcgagccgc ggctgcgcgg cggcgccatc ggccccgagc
     2461 cggtagcgca gcagcagcac gcgcagcagc ccggtcagcg ccggatgggc ggcgccattg
     2521 acgagcagcg cgccgtcgcc ggccagccag tcccgctcgg cgccgccgag cggccacggg
     2581 atttcggacg cgagttcgag cacggccgga tcgtcggccg gcgccggcgc gctgatccgg
     2641 cccggcgcgc tgccgccgag cccgacatag ccccacagca cgcagtgcgc gcggccgccg
     2701 gcgtcgcgca cgagctgcgc gtgcagcggc agcgtcgcct cgccgtcgta cggcgccttc
     2761 agcgccgcgg cgccgagccg ctgcgcggcg accagccggc gctgc
//
LOCUS       DAOQIE010000417         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_24716_length_2795_cov_6.831752, whole genome shotgun sequence.
ACCESSION   DAOQIE010000417 DAOQIE010000000
VERSION     DAOQIE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24716_length_2795_cov_6.831752"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PK144_17675"
     CDS             <1..800
                     /locus_tag="PK144_17675"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF67605.1"
                     /translation="LGAALAPVRVILGGLDALAHGDYDGRLASSGVPELDRIAATVDR
                     LAATLAAGRAENRRLAGHALTVREDERRLLARELHDELGQSLSAIQADATLIAHTATD
                     PRHIESARAIGATTADILGSVRELIRRLHPAVLDVLGLRAALGQLLADWAVRHAPVTT
                     RFDWDPALPERPACGIHAYRIVQEGLTNVARHAAAGRVELRLWREADALRLELADDGR
                     GCDPAAATDGFGLHGIRERAQSFGGHATIESATGQGLRIHVRLPLDG"
     gene            complement(1101..2456)
                     /locus_tag="PK144_17680"
     CDS             complement(1101..2456)
                     /locus_tag="PK144_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNF67606.1"
                     /translation="MDWNQLLSRRRLGIRHRSSAEDTQRTDFQRDFDRIVFASAFRRM
                     QDKTQVFPLSSNDYVRTRLTHSLEVSSVARSLGNRVGEVVIARHGLTQVVPADFGAIV
                     AAAALAHDLGNPPFGHAGEDGIRQFFEETTLGRSLIGRMHPAEQQDLLRFEGNAQGFR
                     VVARLQSPDNFGGMQLTLATLGSFTKYPRGSLLDADPAVGGIAFKKFNYFQADAALFD
                     EVATGLGLDPVLPGAWRRHPLAFLLEAADDICYRIVDLEDAVRLRQLEHGEVLDLLLP
                     LVGLAADSPRLKQLTRPKERIEYLRAKAIGRLIDEVVTLFLDCEAEILAGRYGLELLD
                     EVPSRAPMLEMKELARRRVYIGQPVVEVQVAGFEVLGGLLAAFAGAVTDVAERGGRAT
                     ALNRMLMHLLPEQFVGPGHEPVADAYLRTLKIIDFISGMTDSYAVSLFKRITGMSLPM
                     Q"
     gene            complement(2575..>2795)
                     /locus_tag="PK144_17685"
     CDS             complement(2575..>2795)
                     /locus_tag="PK144_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF67607.1"
                     /translation="AEGRADVYPRLGLTSEWDTAAAQCVVEEAGGHVVGLDGEALPYN
                     TKDSLLNPYFIVYGDGSRDWLSYLPEKV"
BASE COUNT          426 a         1047 c          958 g          364 t
ORIGIN      
        1 tgctcggcgc ggcgctcgcg ccggtgcgcg tgatcctcgg cggcctcgat gcgctcgcgc
       61 acggcgacta cgacggccgg ctcgcatcaa gtggcgtgcc ggagctcgac cgcatcgccg
      121 cgaccgtcga ccgtctggcc gcaacactcg ccgccggccg tgccgagaac cgccgcctcg
      181 ccggccacgc gctgaccgtg cgcgaggacg aacgccggct gctcgcgcgc gagctgcacg
      241 acgagctcgg ccagtcgctg agcgcgatcc aggccgacgc gacgctgatc gcccacaccg
      301 ccaccgaccc gcgccacatc gagagcgcac gcgcgatcgg cgcgaccacg gcggacatcc
      361 tcggctcggt gcgcgaactg atccgccgcc tgcatccggc cgtgctcgac gtgctcggcc
      421 tgcgcgcggc cctcggacag ctgctcgccg actgggcggt gcggcacgcg ccggtcacga
      481 cgcgctttga ctgggaccct gccctgcccg aacgtccggc ctgcggcatc cacgcctacc
      541 gcatcgtgca ggagggcctg accaacgtcg cccgccacgc cgcggccggg cgcgtcgagc
      601 tgcggctgtg gcgcgaggcc gacgccctgc gcctcgaact cgccgacgac ggccgcggct
      661 gcgatccggc ggcggcgacc gacggcttcg gcctgcacgg catccgcgag cgcgcacagt
      721 ccttcggcgg ccacgcgacg atcgaatcgg ccacaggcca gggcttgcgc atccacgtgc
      781 ggctgccgct cgacggctga accggtccgc ggaccggaca ggcgccgctt ccgcccggcc
      841 gtccccgacg ccgccgcatc agcccgatgc ccgcccgcaa ccggctgccg gcgccgtcca
      901 caccgcgccg ctccggtccc cgccgcccca tggcacggtg cgcacccgat cacagcccgc
      961 accggagtcc gccccgcatg ccgtgcccgc ccctgccccg taccccgtac cgcacccgcc
     1021 gcggcccggc gcgaatccgg cacgcccccg atgaacccgc agcgcagacc gcacgggtgc
     1081 agcgcggcaa ccgcatcggc ttactgcatc ggcagcgaca tgccggtgat gcgcttgaac
     1141 aggctgacgg cgtaggaatc ggtcatgccg gagatgaagt cgatgatctt cagcgtgcgc
     1201 aggtaggcat cggccaccgg ctcatgcccg gggccgacga actgctcggg cagcaggtgc
     1261 atcagcatgc ggttcagtgc cgtcgcccgc ccgccgcgct cggcgacatc ggtgacggca
     1321 ccggcgaagg ccgcgagcag gccgccaagc acctcgaagc cggcgacctg cacctcgacg
     1381 accggctggc cgatgtagac acgccggcgc gcgagttcct tcatctccag catcggcgcg
     1441 cggctcggca cttcatcgag cagttcgagc ccgtagcggc cggcgaggat ctcggcctcg
     1501 cagtcgagga acagcgtcac gacctcatcg atcagcctgc cgatcgcctt ggcgcgcagg
     1561 tactcgatgc gctccttcgg ccgggtcagc tgcttcaggc gcggcgaatc ggccgcaagc
     1621 cccacgagcg gcagcagcag gtccagcacc tcgccgtgtt cgagctggcg caggcgcacg
     1681 gcgtcctcga gatcgacgat gcggtagcag atgtcatcgg cggcttcgag caggaaggcc
     1741 agcggatggc gccgccaggc gccgggaagc accggatcga gcccgagccc ggtcgcgacc
     1801 tcgtcgaaca gcgccgcatc ggcctggaag tagttgaact tcttgaaggc gatgccgccg
     1861 actgccggat cggcgtcgag cagcgaaccg cgcggatact tcgtgaagct gccgagggtc
     1921 gccagcgtca gctgcatgcc gccgaagttg tccgggctct gcaggcgcgc gacgacgcga
     1981 aagccctggg cgttgccctc gaagcgcagc aagtcctgct gctcggccgg atgcatgcgc
     2041 ccgatcagcg aacgcccgag cgtcgtttcc tcgaagaact ggcggatgcc gtcctcgccg
     2101 gcatggccga acggcggatt gccgagatca tgcgcgagcg cggccgccgc gacgatcgcc
     2161 ccgaaatcgg ccggcacgac ctgcgtcagc ccgtggcgcg cgatcacgac ctcgccgacc
     2221 cggttgccga gcgagcgcgc gacgctggac acctccagcg aatgcgtcag gcgcgtgcgc
     2281 acgtaatcgt tcgacgacag cggaaagacc tgcgtcttgt cctgcatgcg ccggaacgcc
     2341 gaggcaaaca cgatgcggtc gaagtcgcgc tggaagtcgg tgcgctgcgt gtcctcggca
     2401 gaggatcggt ggcggatgcc gaggcggcgg cgactgagga gctggttcca gtccatcgac
     2461 gggaactccg ggtgacggcg ggggccggca gcgtagcgcg cccgggcgtt gcgtgccgcc
     2521 gtcactgcgt ccggcggccc gtgcggcagg ccacgtgacc gtcagggcgg tcgctcagac
     2581 cttttccggc agatacgaca gccagtcgcg gctgccgtcg ccgtagacga tgaagtaggg
     2641 attcagcagc gaatccttgg tgttgtacgg gagcgcctcg ccatccagcc cgaccacatg
     2701 gccgccagct tcctcgacca cgcactgcgc cgccgccgtg tcccattcgg aagtcaggcc
     2761 caggcgcgga tagacatcgg cacggccttc ggcga
//
LOCUS       DAOQIE010000418         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_25007_length_2775_cov_8.217279, whole genome shotgun sequence.
ACCESSION   DAOQIE010000418 DAOQIE010000000
VERSION     DAOQIE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25007_length_2775_cov_8.217279"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..929)
                     /locus_tag="PK144_17690"
     CDS             complement(99..929)
                     /locus_tag="PK144_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67608.1"
                     /translation="MHDRDTFGNLLLFALIALQLGGPALPFLAQPPGYHAFADTRALA
                     GLANAADVLSNLGFLLAALAGAWTLLRLPATATAVARERDQRRACAVFFAGLALTAAG
                     SAYYHLAPDDGRLVWDRLPMTIVFAGAFASVGAAKYPRVWSARLLVLMLLSAPMSVVW
                     WAVSGNLAWYVAVQFGGLLWLAVSLLALRTRREQQPYVRAWLWALLCYGFAKGFELGD
                     GVIWQSSGGLLGGHALKHLAAALPGFWLVLALRRAAEQQAGRPGAAADGAQLQLQPGT
                     "
     gene            complement(1125..>2775)
                     /locus_tag="PK144_17695"
     CDS             complement(1125..>2775)
                     /locus_tag="PK144_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF67609.1"
                     /translation="HCIGATTLDEYRQYIEKDAALERRFQKVLVDEPSVEDTIAILRG
                     LKERYEVHHKVDITDPAIVAAAQLSHRYITDRQLPDKAIDLIDEAASRIRMEMDSKPE
                     AMDRLDRRLIQLKMERVALKKENDDASRKRLDALETEVTRLEREYADLDEVWKAEKAM
                     LTGAADIKAELEKARLDFDAAKRSGDLNRMSELQYGRIPELERRMKAAEAGESHDFQL
                     LRNRVTDEEIAEVVSKWTGIPVAKMLEGEREKLLRMEDVLGRRVVGQAEAVRAVADAI
                     RRSRAGLADPNRPNGSFLFLGPTGVGKTELCKALASFLFDTEEALVRIDMSEFMEKHS
                     VARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHADVFNVLLQVLDDGRLT
                     DGQGRTVDFRNTVVVMTSNLGSDTIMEMAGEDNYGAMKAAVMAILAGHFRPEFLNRVD
                     ETVVFHPLGREQIRSIAAIQIEGLRKRLAARDIALEVGAGALDKLGEAGFDPVYGARP
                     LKRTIQQMLENPLAKRILAGEFGPGDTVLVEPDLSLRRRAA"
BASE COUNT          402 a         1003 c          958 g          412 t
ORIGIN      
        1 cgacgctgcc gctccccgat gcggccgatc cgcccccggc cctgccggca cccggcgcgg
       61 cagcgcggcc gccctcggtc tgaagccggc gcggcgcttc aggtgccggg ctgcagctgc
      121 agctgcgcac cgtcagcggc cgcgcccggg cgcccggcct gctgctctgc cgcccggcgc
      181 agcgccagca ccagccagaa gcccggcagc gccgcggcga ggtgcttcag cgcgtggccg
      241 ccgagcaggc cgccgctcga ctgccagatc acgccatcgc cgagttcgaa gcccttggcg
      301 aagccgtagc agagcagcgc ccacagccag gcgcgcacgt agggctgctg ctcgcggcgc
      361 gtgcgcagcg cgagcagcga caccgccagc cacagcaggc cgccgaactg cacggccacg
      421 taccaggcga gattgccgct gaccgcccac cacaccacgc tcatcggcgc tgacagcagc
      481 atcagcacca gcagcctggc gctccagacc cgcgggtact tggcggcccc gaccgacgcg
      541 aaggcgccgg caaagacaat cgtcatcggc agccggtccc agaccagccg gccatcatcg
      601 ggcgcgaggt ggtagtacgc ggagccggcc gccgtcagcg ccagtccggc gaagaacacc
      661 gcgcaggccc ggcgctggtc gcgttcgcgg gccacggccg tggccgtggc cggcagccgc
      721 agcagcgtcc acgcgccggc cagcgccgcg agcaggaagc cgaggttcga cagcacgtcg
      781 gccgcattgg cgaggccggc gagcgcgcgc gtgtcggcga aggcgtgata gccgggcggc
      841 tgggccagaa acggcagcgc cgggccgccg agctgcagcg cgatcagcgc gaacagcagc
      901 aggtttccga aggtatcccg atcgtgcatc gccttcctca ctccgtgcat ccggggggtt
      961 ccgggggccg gccgtcgtgg cgcggtgagg cgcgatgatt ccgaagccgc cggacgacgg
     1021 caagcgccgg tgcgggcgcg acggggcgcc tgcaccggcc gcccgccgca aaggaaaacc
     1081 cctgccgcgc gctgcggcag gggtctgcag gcacaagagg atgctcaggc cgcgcgccgg
     1141 cgcagcgaca ggtccggctc gaccagcacc gtgtcgcccg gcccgaactc gccggcgagg
     1201 atgcgcttcg ccagcgggtt ctccagcatc tgctggatcg tgcgcttcag cggccgcgcg
     1261 ccatagaccg ggtcgaaacc ggcctcgccg agcttgtcga gcgcaccggc accgacctcg
     1321 agcgcgatgt cgcgcgccgc gaggcgcttg cgcaggccct cgatctggat cgccgcgatc
     1381 gagcggatct gctcgcgccc gagcgggtgg aacacgacgg tctcgtcgac gcggttcagg
     1441 aactccggcc ggaagtgccc ggcgaggatt gccatcaccg ccgccttcat cgcgccgtag
     1501 ttgtcctcgc cggccatctc catgatcgtg tcggagccga ggttcgaggt catcacgacg
     1561 acggtgttgc ggaagtccac ggtccgcccc tgaccgtcgg tcaggcgccc gtcatcgagc
     1621 acctgcagca gcacgttgaa gacatcggcg tgcgccttct cgatctcgtc gagcaggatc
     1681 acgctgtagg gcttcctgcg cacggcctcg gtcaggtagc cgccttcctc gtaaccgacg
     1741 tagcccgggg gcgcaccgat caggcgcgcg accgagtgct tctccatgaa ctcgctcatg
     1801 tcgatgcgca ccagcgcttc ctcggtatcg aacaggaagc tcgcgagcgc cttgcagagc
     1861 tcggtcttgc cgacgccggt ggggccgagg aacaggaagc tgccgttcgg ccggttcgga
     1921 tcggcgagcc cggcgcgcga gcggcggatg gcgtccgcca ctgcacgcac ggcttcggcc
     1981 tgaccgacga cgcgccggcc gagcacgtct tccatgcgca agagcttctc gcgctcgcct
     2041 tcgagcatct tcgcgaccgg gatgccggtc cacttgctga cgacctcggc gatctcctcg
     2101 tcggtgacgc ggttcctcag cagctggaag tcgtgcgact cgccggcctc ggccgccttc
     2161 atgcgccgct ccagctccgg gatgcgcccg tactgcagct ccgacatgcg gttcagatca
     2221 cccgaacgct tcgccgcatc gaagtcgagc cgcgcctttt cgagttcggc cttgatgtcg
     2281 gccgcgccgg tcagcatggc tttttcggcc ttccagacct cgtcgagatc ggcgtactcg
     2341 cgctccaggc gcgtgacctc ggtctccagc gcgtcgaggc gcttgcgcga ggcatcgtcg
     2401 ttttccttct tcagggccac gcgttccatc ttcagctgga tcagccgccg gtcgagccgg
     2461 tccatcgcct cgggcttgga gtccatctcc atgcggatgc gcgaggccgc ctcatcgatc
     2521 aggtcgatgg ccttgtccgg cagctggcgg tcggtgatgt agcggtgcga aagctgggcc
     2581 gcggcgacga tcgccgggtc ggtgatgtcg accttgtggt ggacctcgta gcgctccttc
     2641 aggccgcgca ggatggcgat ggtgtcctcg accgaaggct cgtcgaccag caccttctgg
     2701 aagcggcgtt cgagcgcggc gtccttctcg atgtactggc ggtactcatc gagcgtggtg
     2761 gcgccgatgc agtgc
//
LOCUS       DAOQIE010000419         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_25456_length_2745_cov_7.197398, whole genome shotgun sequence.
ACCESSION   DAOQIE010000419 DAOQIE010000000
VERSION     DAOQIE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25456_length_2745_cov_7.197398"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1127
                     /locus_tag="PK144_17700"
     CDS             339..1127
                     /locus_tag="PK144_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67610.1"
                     /translation="MADELNLLEAPLFGPSAMLARYKNMSCSVDGGMATVAVNKYRNA
                     NKPSPEKGGCQHAHAVSIRLHEVARDAYERAGGQVRYVEAFMGKGSPATIISVLETFA
                     AYSDAFIAKFAGRKGTPEGKCAAILADGSITWEQTLQQICDEYIGLDCNGFVGNWLRL
                     LQPDLKLTPNHRADEVRTKALHYRTRLDQIEYWDLMCYTHNEHIAAVDDRSPAPGRFM
                     VCQSAGGGPRRNEFAFLQTGTRTFQLAAPTPQDIGYQFYVISLW"
     gene            1303..1695
                     /locus_tag="PK144_17705"
     CDS             1303..1695
                     /locus_tag="PK144_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HNF67611.1"
                     /translation="MHPVVIICVAALGLLLFGLGLAISMQRFRTRTLDGHSAEPTDRL
                     HRLVAAHANTAEFAPYLAVLFLYLGSQAPGTATVALIVAATLCRFLLVLGLLVVGRLD
                     RPNPLRFTGALGTYLLGAALCLVALAGI"
     gene            1729..2745
                     /locus_tag="PK144_17710"
     CDS             1729..2745
                     /locus_tag="PK144_17710"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNF67612.1"
                     /translation="MSQRLPVTVLTGFLGAGKTTLLARLLTQPAAGRCAVIINEFGAV
                     SLDHELIGTVAGDDIVELPGGCLCCARREALGVALADLLLRVRRGEVQPFGRVIIETS
                     GLADPAPILRGLLGDPALMEAFVLDGVVTLVDAVHGAQTLASQPETRAQVALADRLLL
                     SKTDVAEAAAVEALAAQLAALNPLAELETVTHGAIEAARLFGVRVRDEAATAAEVERW
                     LSARGLVNLRPAHAGAPDPNRHGPDLRAEVLLIDEPVPAERLRSFVGALTVLGGDAIL
                     RVKGIVHLAGRPLPFVVHGVQGTYETLQPLADWPSDDRRTRLVVIARGVPAGWAGKLW
                     ESLG"
BASE COUNT          370 a          993 c          943 g          439 t
ORIGIN      
        1 cttcgatctg gcctgatccg cgtggtggct ggcagcgggt gattttccgg tttccgatcc
       61 ctttcccgaa tgcggaaagg gatcggcagc gccgggcact tcgtggccgg atcggccggg
      121 cgggcattgt ccgatgagca cccgccggtc accggtgaag gcgttgttct ttcccggctc
      181 cccgaatcct ttccccgcgc gggacgatgc gccggtcttt gcagcggacg cgatcacggc
      241 cgccgcgaac gtcccccgtg cccggctact ctgaagcggc acgggtcgtg accggctgct
      301 catccccgga ctgccggcgc ccgtgcggag gacatccgat ggccgatgaa ctgaacctcc
      361 tcgaagcccc gctgttcggc ccgtcggcca tgctggcgcg ctacaagaac atgagctgct
      421 cggtcgacgg cggcatggcc acggttgcgg tcaacaagta ccgcaacgcc aacaagccct
      481 cgcccgagaa gggcggttgc cagcatgcgc acgcggtcag catccggctg cacgaagtgg
      541 cgcgcgatgc ctacgagcgt gccggcggtc aggtgcgcta cgtcgaggcc ttcatgggca
      601 agggctcgcc ggccacgatc atcagcgtgc tcgaaacctt cgccgcgtat tcggatgcct
      661 tcatcgccaa attcgcaggc cgcaagggca cgccggaagg caagtgcgca gcgatcctgg
      721 ccgacggcag catcacctgg gagcagacgc tgcagcagat ctgcgacgag tacatcggtc
      781 tcgactgcaa cggcttcgtc ggcaactggc tgcggctgct gcagccggac ctgaagctga
      841 ccccgaacca ccgcgccgac gaggtgcgca ccaaggcgct gcactaccgg acccgtctcg
      901 accagatcga gtactgggat ctcatgtgct acacgcacaa cgaacacatc gccgcggtcg
      961 atgaccgcag ccccgccccc ggcaggttca tggtctgcca gtcggccggc ggcgggccgc
     1021 gcaggaacga attcgccttc ctgcagaccg gcacccggac cttccagctc gccgcgccga
     1081 ccccgcagga catcggctac cagttctatg tgatctcgct ctggtagcgg ctggccggta
     1141 cgcgccccgg aatcacgcga gcgcgcaggg gctgacccgc agggtttcgc aacgctcttt
     1201 caggccgctg gcccggcctt gccggcgaag cttcgccttg cggctgacac tccgggtccg
     1261 acccggcaag atgccggctc ccttgcgcac ggagcgccgt ccatgcaccc ggtcgtcatc
     1321 atctgcgtcg cggccctcgg cctgctgctg ttcgggctgg ggctggcgat ttccatgcag
     1381 cgcttccgca cgcggacgct cgatggccac agcgccgagc cgaccgaccg gctgcaccgg
     1441 ctggtggcgg cgcacgccaa caccgccgag ttcgcgccgt atctcgccgt gctgttcctg
     1501 tacctcggca gccaggcgcc cggcaccgcg accgtcgcgc tgatcgtcgc ggcgacgctc
     1561 tgccgcttcc tgctcgtgct cggcctgctc gtcgtcgggc ggctggaccg gccgaatccg
     1621 ctgcgcttca ccggcgcgct cggcacctat ctgctcggtg cggcgctctg cctcgtcgcg
     1681 ctcgccggca tttgaacgac cgtccttgcg ccacccgggg tgtctttcat gagccagcgc
     1741 cttcccgtca ccgtgctgac cggctttctc ggcgccggca agaccacgct gctcgcacgc
     1801 ctgctgacgc agccggctgc cgggcgctgc gcggtgatca tcaacgagtt cggcgccgtg
     1861 tcgctcgatc acgagctgat cggcacggtg gccggggacg acatcgtcga gctgccgggc
     1921 gggtgtctct gctgcgcgcg gcgcgaagcg ctcggtgtgg cgctcgccga tctgctgctg
     1981 cgcgtgcgcc gcggcgaagt gcagccgttc gggcgcgtga tcatcgagac ctcgggcctc
     2041 gccgatccgg cgccgatcct gcgcggcctg ctcggcgatc cggcgctgat ggaagccttc
     2101 gtgctcgatg gcgtcgtgac gctggtcgat gccgtgcacg gtgcgcagac gctcgcgagc
     2161 cagcccgaga cgcgcgcgca ggtcgcactc gccgatcgcc tgctgctgag caagaccgat
     2221 gtcgccgaag cggccgcggt cgaggcgctc gccgcgcaac tggccgccct gaatccgctg
     2281 gcggagcttg agacggtcac gcacggggcg atcgaggcag cgcggttgtt cggcgtgcgc
     2341 gtgcgcgacg aggccgcgac ggcggccgag gtcgagcgct ggctgtcggc gcgcgggctc
     2401 gtgaacctgc gccccgcgca cgctggcgcg ccggacccga accgccacgg cccggatctg
     2461 cgcgccgagg tgctgctgat cgacgagccg gtgccggccg agcgcctgcg cagcttcgtc
     2521 ggcgcgctga ccgtgctcgg cggcgacgcg atcctgcgcg tcaagggcat cgtgcatctg
     2581 gccggccggc cgctgccctt cgtcgtgcac ggcgtgcagg gcacgtacga aaccctgcag
     2641 ccgctcgcgg actggccctc ggacgaccgc cgcacgcgcc tcgtcgtcat cgcccgcggc
     2701 gtgccggcgg gctgggcggg gaagctctgg gaatcgctgg gctga
//
LOCUS       DAOQIE010000420         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_25859_length_2722_cov_7.522685, whole genome shotgun sequence.
ACCESSION   DAOQIE010000420 DAOQIE010000000
VERSION     DAOQIE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25859_length_2722_cov_7.522685"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PK144_17715"
     CDS             complement(<1..642)
                     /locus_tag="PK144_17715"
                     /inference="COORDINATES: protein motif:HMM:NF017312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HNF67613.1"
                     /translation="MKTTDDAIPVLGWQGEALDCSACAHTDLYAEAGCEPGRACVEDR
                     YAKRIDRFFARNPQLADGQLEHPYFEVRAVACKYASVFRLPAMLADPDETVRASAAMR
                     VPPRQLEALIDDPHREVRIRVATRLDPNRLFAMAADEDYYVRRVVAQRIPASYLPQMR
                     KDPEATVRVEVARRIAPIWVLTMHADADAAVRCEVARRAPVERLGLMARDADCR"
     gene            complement(629..1141)
                     /locus_tag="PK144_17720"
     CDS             complement(629..1141)
                     /locus_tag="PK144_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67614.1"
                     /translation="MPQVWEDAWLARSIEAGLPRQAELALLLAARSYHDPVKAEALLQ
                     EAEAAAPGHLAPLVGLYKFYFYQGRLAQALETAERTLLKVARDLGLPEDWRAVERGSR
                     DFSDYADGPHRMYLFVLKAYGYLLARLGELAGSRAVFEKLRLLDPADRIGASVLLQVL
                     DRGGRDDEDD"
     gene            complement(1342..1689)
                     /locus_tag="PK144_17725"
     CDS             complement(1342..1689)
                     /locus_tag="PK144_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF67615.1"
                     /translation="MSAPTMNLKVSRKAEKFIRRMITFSGKAGGFRLAVKEGGCSGMS
                     YDFSIEAEPAATDAVVTTETGLQVFVPAECQAMLDGIVIECEDSLMNTGLTFFNPNAA
                     KSCGCGTSFTPAA"
     gene            complement(1700..1894)
                     /locus_tag="PK144_17730"
     CDS             complement(1700..1894)
                     /locus_tag="PK144_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF67616.1"
                     /translation="MAYKIIASQCTACSACEPVCPNAAISQKKGVFVINPVDCTECEG
                     QYDEPQCAAVCPVDNTCVPR"
     gene            complement(2070..>2722)
                     /locus_tag="PK144_17735"
     CDS             complement(2070..>2722)
                     /locus_tag="PK144_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNF67617.1"
                     /translation="ACEGDMNMMRHCRQCRADAVGLLGEDRSAEFTLEKLMEKTEIEY
                     DLEARKAYQELVEAERQDKVAAREKEMVEMASVESDTKILIAVATKGGGRINEHFGHA
                     KEFQIYEVSTAGAKFVGHRRVDLYCQGGYGEEEGLDTIIRAINDCTAVFVAKIGGCPK
                     NDLQAAGIDPVDAYAFEYIEQSALAYFKDYVARVESGELVHTARGDAQIRQGAFAA"
BASE COUNT          405 a          897 c          948 g          472 t
ORIGIN      
        1 gcggcagtcg gcatcacgag ccatcaggcc gaggcgctcg accggggcgc ggcgcgcgac
       61 ctcgcagcgc acggcggcat cggcatcggc gtgcatcgtc agcacccaga tcggtgcgat
      121 gcgccgggcg acctcgacgc gcacggtggc ttccgggtcc ttgcgcatct gcggcaggta
      181 gctcgccggg atgcgctgcg cgacgacgcg gcgcacgtag tagtcctcgt ccgcggccat
      241 cgcgaacagg cggttcggat cgagccgggt ggcgacgcgg atgcgcacct cgcggtgcgg
      301 atcgtcgatc agcgcttcga gctgacgcgg cggcacgcgc atcgcggcac tggcacgcac
      361 ggtctcgtcc gggtccgcca gcatcgccgg cagccggaac acgctcgcgt acttgcaggc
      421 gacggcgcgc acctcgaagt acgggtgttc gagctggcca tcggcgagct gcggattgcg
      481 cgcgaagaag cggtcgatgc gcttggcgta gcggtcctcg acgcaggcgc ggcccggctc
      541 gcagccggct tcggcgtaca gatccgtgtg tgcgcaagcg ctgcagtcga gcgcctcgcc
      601 ctgccagccg agcaccggga tggcgtcgtc agtcgtcttc atcgtcgcgt cctccacggt
      661 cgagtacctg cagcagcacg ctggcaccga tccggtcggc cggatcgagc aggcgcagtt
      721 tttcgaacac cgcccggctg ccggcgagtt cgccgaggcg tgcgagcagg tagccgtagg
      781 ccttcagcac gaacagatac atgcggtgcg ggccgtcggc gtaatcggag aaatcgcggc
      841 tgccgcgctc gacggcgcgc cagtcctcgg gcaggccgag gtcgcgggcg accttcagca
      901 gcgtgcgttc ggcggtttcg agcgcctgcg ccagccgccc ctggtagaag tagaacttgt
      961 acagcccgac cagcggcgcc agatgcccgg gcgcggcggc ctcggcctcc tgcagcaagg
     1021 cttcggcctt caccggatcg tggtagctgc gggcggcgag cagcagcgcc agttcggcct
     1081 gccgcggcag gccggcctcg atgctgcggg cgagccaggc gtcttcccag acctgcggca
     1141 tggcggtgct ccggtcgatg gtgtgcggcg cggggcgcgg attggtcagg gccagattcc
     1201 ggcgccggcc gggacgctgc gtccggctgc cgccgcccgt ggcgggcggc ggcagcggca
     1261 gcggccggga cggtgcggac agggtgattt gctggatcat gaccgccgcc ccctcatctc
     1321 tctctcgtgc ggtatccgga ctcaggcagc cggcgtgaac gaggtgccgc agccgcagct
     1381 cttcgcggcg ttggggttga agaaggtcag gccggtgttc atcagcgagt cctcacactc
     1441 gatgacgatg ccatcgagca tggcctggca ctcggccggc acgaacacct gcagaccggt
     1501 ctcggtcgtg accacggcgt cggtggccgc gggttcggcc tcgatgctga agtcatacga
     1561 catccccgaa cagccgcctt ccttgacggc gagccggaag ccaccggcct tgccgctgaa
     1621 ggtgatcatg cggcggatga atttctccgc cttgcggctg accttgaggt tcatcgtcgg
     1681 cgcgctcatg cgggcaggct cagcgcggca cgcaggtgtt gtcgaccggg cagacggccg
     1741 cgcactgcgg ctcgtcgtac tggccttcgc actcggtgca atcgaccggg ttgatcacga
     1801 acacgccctt cttctgggag atggcggcat tcgggcagac cggctcgcag gccgagcagg
     1861 cggtgcactg ggaagcgatg atcttgtagg ccatgacggc agctccttta cggtttgcga
     1921 aacgaaaagc gtggaaacgc tgggtgaaag ccggtactgc cggtcacacg ggactgcctg
     1981 gtgtgtctcc ctttctttcc ggcccctccc ccgcaagtgg cgaggggctc agaaagcgca
     2041 gcgtcccgct gtgaccggcc tgacatgcat caggccgcga aggcaccctg acggatttgc
     2101 gcatcaccgc gcgcggtgtg cacgagttcg ccgctctcga cgcgggcgac gtaatccttg
     2161 aagtacgcca gcgccgactg ctcgatgtat tcgaaggcgt aggcatcgac cggatcgatg
     2221 ccggcggcct gcagatcgtt cttcgggcag ccgccgatct tggcgacgaa caccgcggtg
     2281 cagtcgttga tcgcgcggat gatcgtgtcc agaccttctt cctcgccgta gccgccctgg
     2341 cagtacagat cgacgcggcg gtgaccgacg aacttcgcac cggcggtcga gacttcgtaa
     2401 atctggaatt ccttggcgtg accgaagtgc tcgttgatgc ggccgccgcc cttggtcgcc
     2461 acggcgatca ggatcttggt gtcggattcg acgctggcca tttcgaccat ttccttctcg
     2521 cgcgccgcaa ccttgtcctg acgctcggcc tcgaccagtt cctgataggc cttgcgggct
     2581 tcgaggtcgt attcgatctc ggtcttttcc atcagctttt cgagcgtgaa ttcagccgag
     2641 cgatcctcgc cgagcaggcc gacggcatcg gcgcggcact ggcggcagtg gcgcatcatg
     2701 ttcatgtcgc cttcgcaggc at
//
LOCUS       DAOQIE010000421         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_26203_length_2701_cov_6.906652, whole genome shotgun sequence.
ACCESSION   DAOQIE010000421 DAOQIE010000000
VERSION     DAOQIE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26203_length_2701_cov_6.906652"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..412)
                     /gene="ssrA"
                     /locus_tag="PK144_17740"
     tmRNA           complement(44..412)
                     /gene="ssrA"
                     /locus_tag="PK144_17740"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(558..770)
                     /locus_tag="PK144_17745"
     CDS             complement(558..770)
                     /locus_tag="PK144_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67618.1"
                     /translation="MSADLQLISEALAQQNRLLREIANDLRTLVRVAELSHPEFVDPT
                     PSRADNTIRPASIAVDPGLLPNGGRR"
     gene            complement(899..1381)
                     /locus_tag="PK144_17750"
     CDS             complement(899..1381)
                     /locus_tag="PK144_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNF67619.1"
                     /translation="MNTSPERAPRVFRDWRHFLAFGLGSGLVPVAPGTAGTLAAVPFA
                     WGLAALSPAAYLFIVLAAFALGVYVCGRTSRDLGVHDHGGIVWDEFVGYWITMYMAPP
                     GPGWLIAGFALFRLFDVWKPWPIRIFDVHVPGGLGIMLDDVLAGLYAWVLLQALTAVL
                     "
     gene            complement(1365..2330)
                     /gene="thiL"
                     /locus_tag="PK144_17755"
     CDS             complement(1365..2330)
                     /gene="thiL"
                     /locus_tag="PK144_17755"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNF67620.1"
                     /translation="MTAEFTLIDDLIARRTGAQRADVALGIGDDCALLVPPPGRQLAV
                     SLDLLVAGVHFLPDAEPVALGHKALAVNLSDLAASGAEPAWITLGLTLPGADAGWLAA
                     FMDGFAALAAEHGAQLVGGDTTRGPLCIAVQVHGFVPPGAALTRSGARAGDLVCITGT
                     PGEAAAGLALRFGRLELPEPFATHCLARLDRPTPRIRAGLALRGLASACIDVSDGLAQ
                     DLGHILSASGCGATLALGRLPLTAVNAALGAEAAWPYALAGGDDYELLFTLPAAQQAE
                     AEARLAAAGCPFSVIGRIDPEPGLRLVRPDGRPHALERGGYEHFA"
     gene            2435..>2701
                     /locus_tag="PK144_17760"
     CDS             2435..>2701
                     /locus_tag="PK144_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67621.1"
                     /translation="MPNPRFAVLWLMLMLAPGFAAAAEAAATNGRTPAADAARAEGAA
                     ALSPADLDALARDLDDPQARARLAATLRALAAARRLDAPAAAASS"
BASE COUNT          407 a          916 c         1006 g          372 t
ORIGIN      
        1 ccaaaagaaa aggcccggat ggatcatccg ggccttgctg gtctggtgga gccggcggga
       61 atcgaacccg cgtccgcaag cgctccgccc ttggcactac atgcttagcc cagtcattga
      121 tctgaccgtt ctcgtcccga cgggcagggt ctcgaacggc ggtcccgctt ggttttagcg
      181 cctgggcccc gggcgggctt gcgcgcgatc tcgtgctggt cgacccctga tgtgcccttg
      241 cgggcacccg gacttcacga gcaagttccg gtcagaggct ggccgggtta ggcggccaga
      301 gcgtagttgt cgtcgttggc aactatgagt tgtcgctttt tttacgaggc ctagcgacgt
      361 cctcggcatg cgcctcaggt tttgccaccc acgtcgaaac caggtcggcc cctgaaggga
      421 gggcgactgt acaccgaccg caaggccgct gccagcgccg gtgccgggca gcggacttgc
      481 ggactgcgcg gcaccgggga tcgatatggc ggtgccgcgg cggatttcaa gccgggtgtg
      541 ccggtcgcgt tccggcctca gcgcctgccg ccgttcggca gcagccccgg atcgacggcg
      601 atgctggccg ggcggatggt gttgtctgcg cgcgacggcg tcggatcgac gaactcggga
      661 tgggaaagct cggcgacgcg caccagcgtg cgcaggtcgt tggcaatctc gcgcagcagg
      721 cggttctgct gggccagggc ttcggagatc aactgcaggt cggcactcat gcgtcctcct
      781 gatgcatcag gtcgtggtcc tgacggccgc cggcgggcgg cgcggcaggg tactgatggg
      841 tactcgtcga tacggatcaa ggcttgcggg gtgttgcgca ccttcggttg cgcgcgggtc
      901 acagcacggc ggtcagggcc tgcagcagta cccaggcgta cagtccagcc agtacgtcgt
      961 cgagcatgat gccaagtccg ccgggcacgt ggacatcgaa aatccggatc ggccacggct
     1021 tccagacatc gaacagccgg aacagcgcga agccggcgat cagccagccg gggccgggcg
     1081 gggccatgta catcgtgatc cagtagccga cgaactcatc ccagacgatg ccgccgtgat
     1141 cgtgcacgcc gagatcgcgg ctggtgcgcc cgcagacgta cacgccgagc gcgaaggccg
     1201 cgagcacgat gaacaggtaa gccgccggcg acagcgcggc cagcccccac gcgaacggca
     1261 cggcggcgag cgtgccggcc gtgcccggcg cgaccgggac gaggccgctg ccgaggccga
     1321 aggcgaggaa atgccgccag tcgcggaaga cgcgcggcgc gcgctcaggc gaagtgttca
     1381 taaccgcccc gttccagcgc atgcggccgg ccatcggggc gcacgaggcg caggccgggt
     1441 tcgggatcga tgcggccgat gacgctgaac ggacagccgg cggcggcgag ccgggcttcg
     1501 gcttcggcct gctgcgccgc gggcagcgtg aacagcagct cgtagtcatc gccgccggcg
     1561 agcgcatacg gccacgcggc ctcggctccg agtgcggcgt tgacggcggt cagcggtaat
     1621 cgtccgagcg cgagcgtcgc gccgcagccg ctggccgaga ggatgtggcc gaggtcctgc
     1681 gcgaggccgt cggagacatc gatgcaggcg ctcgccagcc cgcgcagcgc cagcccggcg
     1741 cggatgcgcg gcgtcggccg gtcgaggcgg gcgagacagt gcgtggcgaa gggctcgggc
     1801 agctccagcc ggccgaagcg cagcgccagc ccggcggcgg cctcgccggg cgtgccggtg
     1861 atgcagacca gatcgcccgc tcgcgccccg ctgcgggtca gcgccgcgcc gggcggcacg
     1921 aagccgtgta cctgcacggc gatgcagagc ggcccgcgcg tggtgtcgcc gccgacgagc
     1981 tgcgcgccgt gctcggcggc gagcgcggcg aagccgtcca tgaaggccgc gagccagccg
     2041 gcatcggcgc ccggcagcgt caggccgagc gtgatccacg ccggttcggc gccgcttgcg
     2101 gccagatccg acaggttcac cgccagcgcc ttgtggccga gcgcgaccgg ctcggcatcg
     2161 ggcaggaagt gcacgccggc gaccagcagg tcgaggctga ccgcgagctg ccggcccggc
     2221 ggcggcacga gcagtgcgca gtcatcgccg atgccgagcg ccacatcggc ccgctgcgcg
     2281 ccggtgcgcc gggcgatcag atcgtcgatc agcgtgaact ctgcggtcat cgaggcgtcg
     2341 tttccggggt cgtatgacta ttgcgtcgca acaaaaacag cggcaggaag gtcacagcca
     2401 gactgccgta gcggaatcgg gggagacgta cacgatgcca aatccacgct tcgccgtcct
     2461 gtggctgatg ctgatgctcg cgccgggttt cgccgctgcg gccgaagcgg ctgccacgaa
     2521 cggccggacg ccggcggccg atgccgcgcg cgccgagggc gccgcggcgc tgtcgccggc
     2581 cgatctcgat gcgctggcgc gcgatctcga tgacccgcag gcgcgtgcgc ggctggccgc
     2641 gacgctgcgc gcgctcgccg ccgcccgccg gctcgatgca ccggctgccg ctgcatcgag
     2701 c
//
LOCUS       DAOQIE010000422         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_26311_length_2695_cov_4.938636, whole genome shotgun sequence.
ACCESSION   DAOQIE010000422 DAOQIE010000000
VERSION     DAOQIE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26311_length_2695_cov_4.938636"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2069
                     /gene="csx17"
                     /locus_tag="PK144_17765"
     CDS             <1..2069
                     /gene="csx17"
                     /locus_tag="PK144_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HNF67622.1"
                     /translation="TGHWTDDGFVLESRLDRAALLDFFLHDYRPTPVIAPWNGGSGFY
                     PKDNASGISALETATAARLRPYADTIRLARARLAAAGITTDSPKEEAKAALLARLRAA
                     LPDAALRWLDAAVVLGDGSVRYPPLLGTGGNDGRLDFTNNLMQRLVELMDPASGAPTP
                     LAGQHLPAALFAEAAPGLLDRAIGQFQPGAAGGPNAGPGYFGSAQVNAWDFVLMLEGA
                     LLFGAAVTRRLESGDPAALAFPFAVRPRLAGVATGGAADEAAARGELWLPLWQRPCSY
                     PELSALLAEGRVTLGRRLRGQTGARAARDGLDFARAIASYGADRGLSAFERYAFTMRA
                     GKAYLATPLGRIAVERRPAADLIADLDRNAFLDRLRSHARSDTAAAAVRSAARRLDDA
                     LFALATVPEAGEPAQRALIALGEVQRVLGASRKAHEAVPPVPRLQSDWVQRADDGSAE
                     FRLALALAASGPPVLPMRMALAPVDVDGRVWQPDSREHLGQRALVPLLGAALRRRLLL
                     PQPTPEDAPYETVAARTSGANVTALADFLHAAPQNVAAFDARTLALLRGLALLDPSRP
                     QTVRRQPASTAMLPAACIALLLVFTPRQRLVALDLLDQDCPWPAPREAAARLAAGDVA
                     GAVGLAWRRLRSARLPIPSHPHAPPQLPGLDGPRLLAALTVPLHDAALKNLARRLLRQ
                     PAPSAS"
     gene            2108..>2695
                     /gene="cas7u"
                     /locus_tag="PK144_17770"
     CDS             2108..>2695
                     /gene="cas7u"
                     /locus_tag="PK144_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HNF67623.1"
                     /translation="MSLDFTPLARTPRLLLQAGLRPLQGSRFQPTGFPDLGAASYDAP
                     LTDADGHPALRPMLLVESAQSMANRLEKVCWDEIADDWIAPLRGLPVVKVIDSDGVAL
                     TNSVLEAHRLNSPYILEGSDRSMFERLQAELAGMEEGAVDIRRLASLLLRYDANALLH
                     GVFLAKKELAGGRLRLTRALSAFIEAGDVRRAQSGG"
BASE COUNT          354 a         1045 c          936 g          360 t
ORIGIN      
        1 ccaccggaca ctggaccgac gacggcttcg tgctcgaaag ccggctcgac cgcgcggcgc
       61 tgctcgactt cttcctgcac gactaccggc cgacgccggt gatcgcaccc tggaatggcg
      121 gcagcggttt ctatccgaag gacaacgcca gcggcatcag cgcactcgaa accgcgaccg
      181 ccgcccggct gcgcccgtat gccgacacga tccggctggc ccgcgcccgg ctcgccgccg
      241 ccggcatcac caccgacagc ccgaaggaag aagccaaggc cgcgctgctc gcgcgcctgc
      301 gcgcggcgct gcccgatgcg gcgctgcgct ggctcgatgc tgcggtcgtg ctcggcgatg
      361 gcagcgtgcg ctatccgccg ctgctcggca ccggcggcaa cgacggccgg ctcgacttca
      421 ccaacaacct gatgcagcgc ctggtcgagc tgatggaccc cgccagcggc gccccgaccc
      481 cgctggccgg ccagcacctg ccggccgcgc tgttcgccga agcagcgccg gggctgctcg
      541 accgggcgat cggccagttc cagcccggcg ccgccggtgg cccgaacgcc gggccgggct
      601 acttcggcag cgcgcaggtc aacgcctggg atttcgtgct gatgctcgaa ggcgcgctgc
      661 tgttcggcgc cgcggtcacg cgccggctgg aaagcggcga tccggcggcg ctggcgtttc
      721 cgtttgcagt ccggccgcgg ctggccggag tcgcgaccgg cggtgcggcc gacgaagccg
      781 cggcgcgcgg tgaactctgg ctgccgctgt ggcaacgccc gtgcagctat ccggaactgt
      841 cggccctgct cgccgaaggc cgggtgacgc tcggccggcg cctgcgcggg cagaccggcg
      901 cccgcgccgc ccgcgacggc ctcgacttcg cccgcgccat cgccagctac ggcgccgatc
      961 gcggcctgag cgccttcgag cgttacgcat tcacgatgcg cgccggcaag gcgtatctgg
     1021 ccacaccgct cgggcgcatc gccgtagagc gtcgcccggc ggccgacctg atcgccgatc
     1081 tcgaccgcaa cgcctttctc gaccggctgc gcagccatgc ccgcagcgac accgccgccg
     1141 cggccgtgcg cagcgccgcg cgccggctcg acgatgcgct gttcgcgctg gcaaccgtgc
     1201 ccgaagccgg cgagccggcg cagcgggcgc tgatcgcgct cggcgaggta cagcgcgtac
     1261 tcggcgccag ccgcaaggcg cacgaggccg tgccgccggt gccgcggctg caatccgact
     1321 gggtacagcg ggccgatgac ggcagcgccg aattccggct ggcgctggcg ctggccgcca
     1381 gcggcccgcc ggtgctgccg atgcgcatgg cactggcgcc ggtcgatgtc gatggccggg
     1441 tctggcagcc cgacagccgc gaacacctcg gccagcgcgc gctggtgccg ctgctgggtg
     1501 cggcgctgcg ccggcgactg ctgctgcctc agcccacgcc cgaagatgct ccctacgaaa
     1561 ccgtggccgc ccgcaccagc ggcgccaatg tcacggcact cgccgatttc ctgcacgccg
     1621 cgccgcaaaa cgtcgccgcc ttcgatgccc gcacgctggc gctgctgcgc gggctggcac
     1681 tgctcgatcc gtcgcgcccg cagaccgtcc gccggcagcc ggcttcgacc gccatgctgc
     1741 cggccgcctg catcgcgctg ctgctggtgt tcacgccgcg ccagcgactg gtcgcgctcg
     1801 atctgctcga ccaggactgc ccgtggccgg caccgcgcga agccgccgcc cggctggcag
     1861 ccggcgatgt ggccggtgca gtcgggctgg cctggcggcg gctgcgcagc gcccggctgc
     1921 cgattccttc gcatccgcac gcacccccgc aactgcccgg cctcgacggc ccgcggctgc
     1981 tcgcggcgct gacggtgccg ctgcacgacg cggcgctgaa aaacctcgcc agacgcctgc
     2041 tgcgccagcc cgcgccgtcc gcgtcctgat accgatcaca actgaacgcc cggaaaagga
     2101 acccgccatg tccctcgact tcacccccct cgcccgtacc ccgcgcctgc tgctgcaggc
     2161 cggactgcgc ccgctgcaag gcagccgctt ccagccgacc ggttttccgg acctgggcgc
     2221 cgccagctat gacgcgccgc tcaccgatgc cgatggccat ccggcgctgc ggccgatgct
     2281 gctggtcgag tcggcgcaga gcatggccaa ccggctggag aaggtctgct gggacgaaat
     2341 cgccgatgac tggatcgcgc cgctgcgcgg gctgcccgtg gtcaaggtca tcgacagcga
     2401 cggtgtggcg ctgaccaact cggtgctcga agcccaccgg ctcaattcgc cgtacatcct
     2461 cgaaggcagc gacaggtcca tgttcgagcg gctgcaggcc gaactcgccg gcatggagga
     2521 aggcgcggtc gacatccgcc ggctggcgtc gctgctgctg cgctacgacg ccaacgcgct
     2581 gctgcacggc gtgttcctcg ccaagaagga actggccggt ggccggctgc ggctgacccg
     2641 ggcgctgtcg gcgttcatcg aggccggcga cgtgcgccgg gcgcagagcg gcggc
//
LOCUS       DAOQIE010000423         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_26648_length_2675_cov_6.948473, whole genome shotgun sequence.
ACCESSION   DAOQIE010000423 DAOQIE010000000
VERSION     DAOQIE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26648_length_2675_cov_6.948473"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..941
                     /locus_tag="PK144_17775"
     CDS             315..941
                     /locus_tag="PK144_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF67624.1"
                     /translation="MSIVRPASGAAVTVALAAALLAVAVPATAGDLPAGRVLFNTCLG
                     CHGVPGYTNVYPSYHVPKLGGQHADYLAAALKEYRSGQRAHPTMTANASGLDEAQSAE
                     VAAWLSKLPPSPGQPAAKGDAAAGKEKVTKLGCVACHGADGNSAAGTFPKLAGQHADY
                     LVKALEDYHSGARKNPMMAPMAQNLTAQDRLDLAAWFSSQPQGLSVVK"
     gene            1073..>2675
                     /locus_tag="PK144_17780"
     CDS             1073..>2675
                     /locus_tag="PK144_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HNF67625.1"
                     /translation="MNQKWIAGAVVALIAGLSPVAFAATAAEPVPEDFARRCAALAQL
                     ALPLTDPLTANVVGATSAQLPGQTVHLPAYCRVRAVVRPAIHVEVRLPVDSAWNGRYF
                     QSGCGGLCGDVLTDYPHLFNSSLPGLARGYATATSDTGHAGSNVDGRWGYASPQAEED
                     FAYRSIGATATLAKAVIRQFYAPRTAVPSIFSGCSTGGRLAVKAALKYPNEFNGIVAG
                     APGLDYPGLVGTAFAWLERANTGPDGGRVFNAGNTDKAKLIYETTLAQCDGLDGVVDG
                     SIADPRRCRPDLSALACGLADGPQCLSSVELRTLELWRRGAQDRFGRPLYPGGVPEGS
                     ELYWGLWLTGAPAYAAAFPNGFVPPIALDFLRYLAFETDPGPAYQLTDFDFDRDPARM
                     SASAALLNADDPDLGAFQKAGGRMLMYHGWADPLVTPYKSVDYFEAVAARAGGSAAAD
                     GFVRLFMIPGMDHCGVQSAASPGPGVDERSIDPLTALEHWLFDGSAPDTLTMHAWGPD
                     NSSVAWSRPICRFPQRTVLKDGAGDW"
BASE COUNT          378 a         1016 c          904 g          377 t
ORIGIN      
        1 cgccgtcctt catgccggtg gcgcgccaca gcgcgcgggc gcccgccatg ttgcggccgt
       61 gggtggtggt gcgggaacgg tactcgggca tgggggcact ccggggcgga tcggcgcgtt
      121 cgggccgcgg cgcatcgaac gcatcaggcg ccgcggcggt gtggggccgg gcatttcagc
      181 acagcgcgcc ggcagcggca aaagccgtgc gactgtacgc attccctgag cttgcaagcg
      241 gcgggatcgt caatagtctt gaacgccgaa ggcccgcaga aagcccgccc cctgcccacc
      301 aagcgagaac ccgcatgtcc atcgtccgtc ctgcttccgg cgccgccgtc accgtcgccc
      361 ttgccgccgc gctgctggcc gtcgccgtcc ccgccaccgc cggcgacctg ccggccggcc
      421 gggtgctgtt caacacctgc ctcggctgcc acggcgtgcc cggctacacc aacgtctatc
      481 cgagctatca cgtgccgaaa ctcggcggcc agcacgccga ttacctcgct gcggccctca
      541 aggaataccg cagcggccag cgcgcgcatc cgacgatgac cgccaacgcc agcggcctcg
      601 acgaggccca gagcgccgag gtcgccgcct ggctgtcgaa gctgccgccg tccccgggcc
      661 agccggccgc gaagggcgac gctgccgccg gcaaggagaa ggtcacgaag ctcggctgcg
      721 tcgcctgcca cggcgccgac ggcaactccg cggccggcac cttcccgaag ctcgccggcc
      781 agcacgccga ctatctggtc aaggcgctgg aggactacca ctccggcgcg cgcaagaacc
      841 cgatgatggc gccgatggcg cagaacctga cggcgcagga ccgcctcgac ctcgccgcct
      901 ggttctccag ccagccgcag gggctgtcgg tcgtcaagta agcagccagg ccggcggctt
      961 tggtctgacc agaaccgcat cccctgcccc gtccgtggca gctcttcccg gcctcgcata
     1021 ggctcgcaat gttgcctcgc aacaatgcaa ggaaccgcca ggagagctgc cgatgaacca
     1081 gaagtggatt gcaggcgcgg tcgtcgcgct catcgccgga ctgtcgccgg tcgcgttcgc
     1141 cgcgaccgca gcggaaccgg tgcccgagga cttcgcgcgg cgctgcgccg cgctcgccca
     1201 gctggcgctg ccgctgaccg atccgctgac ggcgaacgtc gtcggcgcca cctccgccca
     1261 gctgcccggc cagaccgtcc acctgccggc ctactgccgc gtgcgcgccg tcgtgcgtcc
     1321 ggccatccac gtcgaggtgc gcctgccggt cgattcggcg tggaacggcc gttacttcca
     1381 gtccggctgc ggcggcctct gcggcgacgt gctgaccgac tacccgcatc tgttcaactc
     1441 cagcctgccg gggctcgcgc gcggctatgc gaccgccacc agcgacaccg gccacgccgg
     1501 cagcaacgtc gacgggcgct ggggctacgc cagtccgcag gccgaggagg acttcgcgta
     1561 ccgctcgatc ggcgccaccg cgacgctcgc caaggccgtg atccgccagt tctacgcgcc
     1621 gaggactgcg gtgccgtcga tcttctccgg ctgctcgacc ggcgggcggc tggcggtcaa
     1681 ggcggcgctg aaatacccga acgagttcaa cggcatcgtc gccggcgcgc ccggcctcga
     1741 ttaccccggc ctggtcggca ccgccttcgc gtggctggag cgcgccaaca ccggcccgga
     1801 cggcgggcgc gtgttcaacg ccggcaacac cgacaaggcg aagctgatct acgagaccac
     1861 gctcgcgcag tgcgacgggc tcgatggcgt cgtcgacggc tcgatcgctg atccgcgccg
     1921 ctgcaggccg gatctgtccg ccctcgcctg cggcctggcc gacggcccgc agtgcctgag
     1981 cagcgtcgag ctgcgcacgc tcgaactctg gcgtcgcggc gcgcaggacc gcttcggccg
     2041 gccgctgtat ccgggcggcg tgccggaagg ctcggagctg tactggggcc tgtggctgac
     2101 cggcgccccg gcctatgccg cggccttccc gaacggcttc gtgccgccga tcgcgctcga
     2161 cttcctgcgc tatctggcct tcgagaccga cccgggaccg gcctatcagc tcaccgactt
     2221 cgacttcgac cgtgacccgg cgcgcatgag cgcctcggcg gcactgctga acgccgatga
     2281 tccggacctc ggtgccttcc agaaggccgg cggccgcatg ctgatgtatc acggctgggc
     2341 cgatccgctg gtcacgccgt acaagagcgt cgattacttc gaggcggtcg ccgcccgggc
     2401 cggcggcagc gccgcggccg acggcttcgt gcgtctgttc atgatcccgg gcatggatca
     2461 ctgcggcgtg cagtcggccg ccagccccgg tcccggcgtc gacgagcgca gcatcgatcc
     2521 gctgaccgcg ctcgaacact ggctgttcga cggcagcgcc ccggacacgc tgacgatgca
     2581 cgcctggggc cccgacaaca gcagcgtcgc ctggagccgg ccgatctgcc gcttcccgca
     2641 gcgcacggtg ctgaaggacg gcgccggcga ctggc
//
LOCUS       DAOQIE010000424         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_26799_length_2666_cov_8.569131, whole genome shotgun sequence.
ACCESSION   DAOQIE010000424 DAOQIE010000000
VERSION     DAOQIE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26799_length_2666_cov_8.569131"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PK144_17785"
     CDS             <1..463
                     /locus_tag="PK144_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNF67626.1"
                     /translation="LVVPSGYSFNLDGTNIYLTMAALFVAQALDIELSLREQLTLLGV
                     AMLTSKGASGVTGAGFVTLAATLAVVPSIPVAGLALILGIDRFMSEARALTNLIGNGV
                     ATIVIAKWEKELDETALHAHLDGRANVEAEIEADAHGERAPLVTATPAQAV"
     gene            733..1746
                     /locus_tag="PK144_17790"
     CDS             733..1746
                     /locus_tag="PK144_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNF67627.1"
                     /translation="MLRITLKTLTLGVALALGGLAHAADPILIKFSHVVAQDTPKGKA
                     AEYFKKLAEERTKGAVKVEVYPNSQLYKDKEEVEALQLGAVQMLAPSLAKFGPLGVKE
                     FEVFDLPYILPNRDVLRRVTDGPVGRGLLDKLETKGIKGLAYWDNGFKIMSANKPIHL
                     PADMKGLKVRIQSSKVLDKQMRELGAIPQVMAFSEVYQALQTGVVDGTENPPSNMYTQ
                     KMHEVQKDATVTRHGYLGYAVIVNKKFWDDLPADIRTTLEGAMKDATKYANDIAEKEN
                     ADALEAIKASGKTTVYTPTDAELKQWRDALLPVHAEVASRVGKDLIDAFYKEAAAAGA
                     PIK"
     gene            1838..2482
                     /locus_tag="PK144_17795"
     CDS             1838..2482
                     /locus_tag="PK144_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNF67628.1"
                     /translation="MKFLDHLEEWLIAFLIAGATLIVFVSVVHRYLAGMAIPGLQDWL
                     LSLDFSWAQELCIFMFIWMAKFGAAYGVRTGIHVGVDVLINRLSTPMRAKFVLFGLLA
                     GATFTGIVGTFGAGLVAHVMHTGSTTEVLEWPAWILYMAIPLGSYLMCFRFLQVAWAF
                     HRTGDLPHHDHGHVEGVDDPDKPEDINWFALDDDLHPREMSHPIRKPEDGGKPQ"
     gene            2479..>2666
                     /locus_tag="PK144_17800"
     CDS             2479..>2666
                     /locus_tag="PK144_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNF67629.1"
                     /translation="MNALIIFGLLFGLMLTGMPISISLGLTVLTFLFGFTNTDVQTVA
                     LKLFTGIEKFEIMAIPFF"
BASE COUNT          467 a          943 c          839 g          417 t
ORIGIN      
        1 gctggtggtg ccgtccggct attcgttcaa cctcgacggc accaacatct acctgaccat
       61 ggcggcgctg ttcgtcgcgc aggcgctgga catcgaactc agcctgcgcg aacagctgac
      121 cttgctcggc gtcgcgatgc tgacctccaa gggcgcctcg ggggtgaccg gcgcgggctt
      181 cgtcacgctc gccgcgaccc tggccgtggt gccgtccatc ccggtcgcgg gactggcgct
      241 gatcctgggc atcgaccgct tcatgtccga ggcccgcgcg ctgaccaacc tgatcggcaa
      301 cggcgtggcg accatcgtca tcgccaagtg ggagaaggaa ctcgacgaga cggcgctgca
      361 cgcccatctc gacggccgcg ccaacgtcga ggccgagatc gaggccgacg cgcacggcga
      421 gcgtgcaccg ctggtcacgg cgacgccggc gcaggccgtc tgaagcccga cccgcccgga
      481 cccttcgcgg tccgggcaac ccagcggacc ggtccacgac cggtccgtgc cgtgtccggc
      541 tcaccggcat ttgccgccgg tcagccggta ttaccgcggg cagcgtccta agctgtccgg
      601 cgcacgggcc gccatcgggc agcggcccct gcgtacccgc cccgtcaccc cccgggcggc
      661 cggggcatca ctccaagccc gaaacgcgat cgttccgaag attccaatta ttagagacga
      721 ggagaaaaac ccatgctgcg catcacgctc aagaccctga ccctcggcgt cgccctggcg
      781 ctcggcgggc tcgcccacgc ggccgatccg atcctcatca agttctccca cgtcgtcgcc
      841 caggacacgc cgaagggcaa ggcggccgaa tacttcaaga agctggccga ggagcgcacc
      901 aagggggcgg tcaaggtcga ggtctacccg aacagccagc tctacaagga caaggaggag
      961 gtcgaggcgc tgcagctcgg cgccgtgcag atgctcgcgc cttcgctcgc gaagttcggg
     1021 ccgctcggcg tcaaggaatt cgaggtcttc gacctgccct acatcctgcc gaaccgcgac
     1081 gtgctgcgcc gcgtcaccga cgggccggtc gggcgcggcc tgctcgacaa gctggagacc
     1141 aagggcatca agggcctggc ctactgggac aacggcttca agatcatgtc ggcgaacaag
     1201 ccgatccatc tgccggccga catgaagggc ctgaaggtgc gcatccagtc ctcgaaggtg
     1261 ctggacaagc agatgcgtga gctcggcgcg atcccgcagg tgatggcctt ctccgaggtc
     1321 taccaggcgc tgcagaccgg cgtcgtcgac ggcaccgaga acccgccgtc gaacatgtac
     1381 acgcagaaga tgcacgaggt gcagaaggac gccacggtca cgcgccacgg ctatctcggc
     1441 tacgcggtca tcgtcaacaa gaagttctgg gacgacctgc cggccgacat ccgcacgacg
     1501 ctcgaaggcg cgatgaagga cgccaccaag tacgcgaacg acatcgccga gaaggaaaac
     1561 gccgacgcgc tcgaagcgat caaggcctcc ggcaagacca ccgtctacac gccgaccgat
     1621 gccgagctga agcagtggcg tgatgcactg ctgccggtgc atgccgaggt cgcctcgcgc
     1681 gtcggcaagg acctgatcga cgccttctac aaggaagccg ctgccgccgg cgcgccgatc
     1741 aagtaagccg cccccgcgtc cgcacctcgc cggcccggcg gccctgccgc cgccggccgg
     1801 cgccccgccc gacccgttgt gcgcggagga cccccgcatg aaattcctcg atcacctgga
     1861 ggaatggctg atcgccttcc tcatcgcggg tgcgacgctg atcgtgttcg tcagcgtcgt
     1921 gcaccgctac ctcgccggca tggccatccc cggcctgcag gactggctgc tgtcgctcga
     1981 cttctcgtgg gcgcaggagc tctgcatctt catgttcatc tggatggcca agttcggcgc
     2041 cgcctacggc gtgcgcaccg gcatccacgt cggcgtcgac gtgctgatca accggctgtc
     2101 gacgccgatg cgcgcgaagt tcgtgctgtt cgggctgctc gccggcgcga ccttcaccgg
     2161 cattgtcggc accttcggcg ccgggctggt cgcgcacgtg atgcacaccg gcagcacgac
     2221 tgaggtgctc gaatggccgg cgtggatcct gtacatggcg atcccgctcg gctcctacct
     2281 gatgtgcttc cgcttcctgc aggtggcctg ggccttccac cgcaccggcg atctgccgca
     2341 ccacgaccac ggccacgtcg agggcgtcga cgatcccgac aagcccgaag acatcaactg
     2401 gttcgcactc gacgacgacc tgcacccgcg cgagatgtcg cacccgatcc gcaagcctga
     2461 agacggaggc aagccgcaat gaatgccctg atcatcttcg gactgctgtt cggactgatg
     2521 ctgaccggca tgccgatctc gatttcgctc ggcctgacgg tgctgacctt cctgttcggc
     2581 ttcacgaaca ccgacgtgca gaccgtcgca ctgaagctgt tcaccggcat cgagaagttc
     2641 gagatcatgg cgatcccgtt cttcat
//
LOCUS       DAOQIE010000425         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_26924_length_2659_cov_7.943548, whole genome shotgun sequence.
ACCESSION   DAOQIE010000425 DAOQIE010000000
VERSION     DAOQIE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26924_length_2659_cov_7.943548"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="rtcA"
                     /locus_tag="PK144_17805"
     CDS             complement(<1..975)
                     /gene="rtcA"
                     /locus_tag="PK144_17805"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNF67630.1"
                     /translation="MSSDFLLIDGAAGEGGGQILRSALSLSMLSGRAFRIERIRAGRE
                     RPGLLRQHLTAVQAAADLCAAEVEGAQAGSQTLAFRPGPIRAGDWHWSIGTAGSCTLV
                     LQTVLPALWHAPAPSRVRIEGGTHNPAAPPADFLQRAWLPLLAGIGLHADLRLLRHGF
                     YPAGGGAIEVTVTPAATLTPLRLEQRGTLRSTGAEALFAALPAGIAERELAEVARRLG
                     WPAESLKLRGLPAERGPGNALLLTLEHEALTEVFTAYGERGVSAETVARRVSNTAARY
                     LASRAAVGPYLADQLLLPLALAGGHFTTVEPSAHTRSNLDVIRQFLPDL"
     gene            complement(1026..1661)
                     /gene="prfH"
                     /locus_tag="PK144_17810"
     CDS             complement(1026..1661)
                     /gene="prfH"
                     /locus_tag="PK144_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HNF67631.1"
                     /translation="MNAAGGWLQLSSGRGPAECGWVIAQLLPKLVAAATAAGLTAKLL
                     DSSPGPLPGTLASALLAVDGEGFEPFARDWTGSVQWIGTSPFRPAHKRRNWFVAIDRL
                     DAPRELPWRADDVRVETLRAGGPGGQHVNRTDSAVRLTHRPSGLQVLAREERSQHQNR
                     RLAEQRLAARLAALARTDRSDTDAERWRCHDRLIRGNARRVFVGPGFDERG"
     gene            complement(1658..>2659)
                     /locus_tag="PK144_17815"
     CDS             complement(1658..>2659)
                     /locus_tag="PK144_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HNF67632.1"
                     /translation="LHPGKVTPIGAAFASVGLIHPALVGNDIGCGMGLWTTELKRAKA
                     KPAALAGRLHGLDGPWDGDTAARLAAAGLAPTVCDAALGTIGGGNHFAELQAIEAIHD
                     APAVAALGLEAERLLLLVHSGSRGLGEHILRAHVERHGHAPLAADSDDGRAYLEQHEH
                     ARRWAIENRALIAARISDCLRTTGERRLDVCHNSVSAALVDGCACWLHRKGAAPADAG
                     PVVVPGSRGSFSYLVQPAPDSEATLYSIAHGAGRRWKRSDTRARLQRRFTADDLTRTA
                     LGSEVVCEDRELLYEEAPPAYKDIDTVIRDLVDAGLARVLAVLRPLVTYKLRARVRA"
BASE COUNT          322 a          938 c         1042 g          357 t
ORIGIN      
        1 cagatcgggc aggaactggc ggatcacgtc gaggttgctg cgcgtgtgcg ccgagggttc
       61 gacggtcgtg aagtggccgc cggccagcgc cagcggcagc agcagctgat cagccagata
      121 cgggccgacc gcggcgcggc tggccagata gcgcgcggcg gtgttgctga cgcggcgggc
      181 gacggtttcg gcgctgacgc cgcgctcgcc gtaggccgtg aacacctcgg tcagcgcctc
      241 gtgttcgagc gtcagcagca gcgcgttgcc ggggccgcgc tcggccggca ggccgcgcag
      301 cttcaggctt tccgccggcc agccgaggcg gcgggcgact tcggcgagtt cgcgttcggc
      361 gatgccggcc ggcagggccg cgaacagggc ttcggcaccg gtgctgcgca gcgtgccgcg
      421 ctgttcgagt cgcagcggcg tcagcgtggc ggccggcgtc accgtgactt cgatggcgcc
      481 gccgccggcg gggtagaagc cgtggcgcag cagcctgaga tcggcgtgca ggccgatgcc
      541 ggcgagcaac ggcagccagg cgcgctgcag aaagtcggcc ggcggcgcgg ccgggttgtg
      601 cgtgccgccc tcgatgcgca cgcggctcgg agcgggcgcg tgccagagcg ccggcagcac
      661 ggtctgcagc accagcgtgc aggagccggc ggtgccgatg gaccagtgcc agtcgccggc
      721 acggatcggg ccggggcgga aggcgagcgt ctggctgccg gcctgcgcgc cttcgacttc
      781 ggcagcgcag aggtcggcgg cggcctgcac ggcggtcagg tgctggcgca gcaggccggg
      841 ccgctcgcgg ccggcgcgga tgcgctcgat gcggaaggcg cggccgctca gcatcgacag
      901 gctgagcgca ctgcgcagga tctgtccgcc gccttcgccg gcggccccgt cgatcagcag
      961 gaaatcggat gacatcggat cggttcctta tctatgtgcc gtgccggatg tgccgtgccg
     1021 gtccgtcagc cgcgctcgtc gaagcccggg ccgacgaaca cgcgccgggc gttgccgcgg
     1081 atcaggcggt cgtggcagcg ccagcgttcg gcgtcggtgt cgctgcgatc ggtgcgggcg
     1141 agggcggcca gccgcgcggc gaggcgttgt tcggcgaggc ggcggttctg gtgctgcgag
     1201 cgttcctcgc gcgccagcac ctgcaggccg ctcgggcggt gggtcaggcg cacggcgctg
     1261 tcggtgcggt tgacgtgctg gccgccgggg ccgccggcgc gcagcgtctc gacgcgcacg
     1321 tcatcggccc gccacggcag ttcgcgcggc gcatcgagcc ggtcgatggc gacgaaccag
     1381 ttgcgccgct tgtgcgccgg ccggaacggg ctggtgccga tccactgcac gctgccggtc
     1441 cagtcgcgcg cgaacggctc gaagccttcg ccgtcgacgg cgagcagcgc cgatgccagc
     1501 gtgccgggca gcgggccggg gctgctgtcg agcagcttcg ccgtcagccc ggcggccgtg
     1561 gcggcggcga cgagcttcgg cagcagttgc gcgatcaccc agccgcattc ggccgggccg
     1621 cgcccgctgc tgagctgcag ccagccaccg gcggcgttca tgcccgcacc cgcgcgcgca
     1681 gcttgtaggt gacgagcggg cgcagcaccg ccagcacgcg cgccagcccg gcatcgacga
     1741 ggtcgcggat caccgtgtcg atgtccttgt aggcgggcgg ggcttcctcg tagagcagtt
     1801 cgcggtcctc gcagaccact tcgctgccga gcgcggtgcg ggtcaggtca tcggccgtga
     1861 agcgccgctg cagccgcgcg cgcgtgtcgc tgcgcttcca ccggcgcccg gcgccgtggg
     1921 cgatcgaata cagcgtcgcc tcgctgtcgg gcgccggctg caccagatag ctgaagctgc
     1981 cgcgcgaacc cggcacgacc accggcccgg catcggcggg cgcggcgccc ttgcggtgca
     2041 gccagcaggc gcagccgtcg acgagcgcgg ccgagacgct gttgtggcag acatcgagcc
     2101 ggcgctcgcc ggtggtgcgc aggcagtcgc tgatgcgcgc ggcgatcagc gcgcggttct
     2161 cgatcgccca gcgccgggcg tgctcgtgct gttcgagata cgcccggccg tcgtcgctgt
     2221 cggcggcgag cggcgcgtgg ccgtggcgct cgacgtgcgc gcgcaggatg tgttcaccga
     2281 ggccgcgcga gccgctgtgc acgagcagca gcaggcgctc ggcctccagc ccgagcgcgg
     2341 cgacggccgg cgcatcgtgg atcgcctcga tcgcctgcag ctcggcgaag tgattgccgc
     2401 cgccgatggt gccgagcgca gcatcgcaca cggtcggcgc cagtcccgcg gcggcgaggc
     2461 gcgcggcggt atcgccatcc cacggcccgt cgagcccgtg cagccgcccg gccagcgccg
     2521 ccggcttcgc cttcgcgcgc ttcagttcgg tcgtccagag gcccatgccg cagccgatgt
     2581 cgttgccgac cagcgccgga tggatcagcc cgacgctcgc aaacgccgca ccgatcggcg
     2641 tgaccttgcc ggggtgcag
//
LOCUS       DAOQIE010000426         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_27419_length_2630_cov_7.914951, whole genome shotgun sequence.
ACCESSION   DAOQIE010000426 DAOQIE010000000
VERSION     DAOQIE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27419_length_2630_cov_7.914951"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            907..>2630
                     /locus_tag="PK144_17820"
     CDS             907..>2630
                     /locus_tag="PK144_17820"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67633.1"
                     /translation="MAELPAVQVLSGRLEAGQSVLFALPGLRAGTQLRFRAEGLDGSL
                     DPLLAVLKPGLDLERARIDYRERYQRLRAGRTEDPAAYAALLDEYVLAQADDSAGGYA
                     ADLEFTVPADGDYRLLLSASPLRPGFGGYRLTIAGEEGIAAVSVLAGVWGGDARVQAT
                     GLAFTGDEPWRYVELRPVQAGETLQVRLEAVDGAPLPPQLLLLDYGDKPLATASTSTS
                     TAAPARLTLTHEFRSADPAPRLRVARPDGASGAQYRLLLGIDTAAFGDGPVEPLGRPL
                     LQVPIEVQVGVRMDQITGVDQRGENFGAVASLRMDWTDPALAFNPESCGCRLKTFGGD
                     AFAQYVQQHGALWPEFVVFNQQGNRWAQKRLVVLRPDGSAQYYERFSTTLQAPDFDFR
                     RFPFDTQRFFIRVELIAPEWKFRFVELPGYSGLGRQLGEEEWVTTGFDTRISSETETT
                     GLPVSRFSYGFTAERHLNYYAYRIFLPVLVIIVIAWVTLFLRDYGKRVEVAGGNLLLF
                     IAFNFTISSDLPRLGYLSFLDTLLLSAFVVTGCMFVLAVYMRRLEAGGASAVLQRIDG
                     WVIWLYPLA"
BASE COUNT          391 a          842 c          881 g          516 t
ORIGIN      
        1 gcctgcgtgt ggccggtcag gatgcgggcg gaagcggatt ccatcgatga ctcctcggcg
       61 gtattcacat ggcggggtcc ttcctgcgct ttctggtttg caccggattc cgggctgctc
      121 cggcgggcgg cattacaggc cattggggtt ttccggaagg aatgtcggaa ttgccgtagc
      181 gacattgatt gctattgcat ttgcatcatt tcggtttctg aagcgttccg ggtgcagcag
      241 cccatccgcc tgtccatgtc agaaacgggc ctgcagggcc ttgtcgcgag gtctgtaccg
      301 atacagatgc ctgcaggacg tactgaggca actgcttgaa cagtatgcac tgagcacggc
      361 aataatggac agaagcttgt ggtcatggcc tttgcagcgg cgttcatggt ctggattgtg
      421 cggtgcaaaa tcgacaagtc ttcgtgacgg cacggttgtc ggcgtaggtg tcggcggtgg
      481 tggtcgcggc cgttgaagcg cggaattcct ccgtaagcaa gccatatgcc attgccgtca
      541 agcacggttg agccggtctg cctgcgcaat cattgcaagc cttgcatatc ggcccaggtt
      601 caatggaatt aagcgtttgc tctcgtttca gcaggcagtt tcaccagcgg ccacattttt
      661 gcggtgcgtc aattagcgca ggcgaagcgg tgcgtgccgg gggcaattcg cggtttgcaa
      721 cgctcctgcg cccatgccgg aacacatcgt gcgtcagtcg ttgcagttct gtaccgctac
      781 gctgtgacgg atcgaatcct ccccggacct gcaccccgaa tgtctccgct ccctggctgt
      841 cgccgtctca tcgtcttgtg ctggctgtgt gtatgtgcag ggctcgcctc gtggcccccc
      901 ggggccgtgg ctgaacttcc tgcggtgcag gtcctgagcg gccggctcga agccgggcag
      961 agcgtgctgt ttgcactgcc gggactgagg gccggcacgc agctgaggtt tcgggccgag
     1021 ggcctcgacg gcagtctcga tccgctgctc gcggtgctga agccggggct cgatctcgag
     1081 cgggcgcgca tcgactaccg cgagcgctac cagcgcttgc gtgccgggcg caccgaggac
     1141 ccggcagcct acgcggcgct gctcgacgag tacgtgctcg cgcaggccga cgacagcgcc
     1201 ggcggttatg cggccgatct ggagttcacg gtgccggccg acggcgacta ccggctgctg
     1261 ctgagcgcaa gtccgctgcg tccgggcttc ggcggctacc ggctgacgat cgccggcgag
     1321 gagggaatcg cggcggtcag cgtcctggcc ggtgtctggg gcggcgatgc gcgcgtgcag
     1381 gcgaccggac tcgccttcac cggcgacgaa ccctggcgct atgtcgagct gcggccggtg
     1441 caggccggcg agacgctgca ggtgcggctc gaagccgtcg acggggcgcc gctgccaccg
     1501 caactgctgc tgctcgacta cggcgacaaa ccgctcgcca ccgccagcac cagcaccagc
     1561 accgctgcgc cggcgcgact gacgctgacg catgagtttc gcagcgccga cccggcaccg
     1621 cgcctgcgcg tcgcgcgtcc cgatggcgcg agcggcgcgc agtaccggct gctgctcggc
     1681 atcgacaccg ccgcgttcgg cgacggtccg gtcgagccgc tcggccggcc gctgctgcag
     1741 gtgccgatcg aggtgcaggt cggtgtgcgc atggaccaga tcaccggcgt cgaccagcgt
     1801 ggcgagaact tcggtgccgt cgcctcgctg cgcatggact ggaccgaccc ggcactggcc
     1861 ttcaatcccg agtcctgcgg ctgccgtctg aagaccttcg gcggcgacgc cttcgcgcag
     1921 tacgtgcagc agcacggcgc gctgtggccg gagttcgtcg tgttcaacca gcagggcaac
     1981 cgctgggcgc agaagcgcct cgtcgtgctg cgcccggacg gttccgcaca gtattacgag
     2041 cgcttctcga cgacgctgca ggcaccggat ttcgatttcc ggcgttttcc gttcgatacg
     2101 cagcggtttt tcatccgcgt cgaactgatc gcgccggagt ggaaattccg tttcgtcgaa
     2161 ctgcccggct acagcggtct cggccgccag cttggcgagg aggaatgggt cacgaccggt
     2221 ttcgacaccc gcatcagcag cgagaccgaa accaccggcc tgccggtatc gcggttttcc
     2281 tacggcttca cggccgagcg gcacctgaat tactacgcgt accggatctt cctgccggtg
     2341 ctggtgatca tcgtcatcgc ctgggtgacg ctgttcctgc gcgactacgg caagcgcgtc
     2401 gaggtcgccg gcggcaacct gctgttgttc atcgccttca acttcacgat ctccagcgac
     2461 ctgccacggc tcggctacct gagcttcctc gatacgctgc tgctcagcgc cttcgtcgtc
     2521 accggctgca tgttcgtgct cgccgtgtac atgcgcagac tggaggccgg cggggccagc
     2581 gctgtgctgc agcgtatcga cggctgggtg atctggctgt atccgctggc
//
LOCUS       DAOQIE010000427         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_27725_length_2612_cov_5.112632, whole genome shotgun sequence.
ACCESSION   DAOQIE010000427 DAOQIE010000000
VERSION     DAOQIE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27725_length_2612_cov_5.112632"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..222
                     /locus_tag="PK144_17825"
     CDS             28..222
                     /locus_tag="PK144_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67634.1"
                     /translation="MADILPFKRPKPADRMEAAAGRELCKSGFHKWEVQKDKRFDVKA
                     GRLVTSYRCARCGATRTEGR"
     gene            312..1256
                     /locus_tag="PK144_17830"
     CDS             312..1256
                     /locus_tag="PK144_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67635.1"
                     /translation="MPRLLASLVLALGLSLPAAAADLAKLRAAFASVRFIAYSPSAYT
                     DGGLVGRDAIRRDLLILAPDFDGIVTYASDNGSEQVAALARETGIRAVILGVHNPANT
                     TELDTAIRLAREYPEVVVAICVGNEGLISHRYDWPTLRRGLDHVRAALPELPLTTGEP
                     FVIHLEWTPPGFFDAQDFIGVNIHPLFEGWFTPDKLDAGMDYLDAILGKVIARAPGKP
                     VMIKETGVPGQPGGEWSPAVQAGFWTRVERRFPRNGDRAFSAFEAFDAPWKPALIARD
                     FKDYGGTVDPREAYWGFYTVDGEPKPVVGELRAQRRGP"
     gene            complement(1303..2376)
                     /locus_tag="PK144_17835"
     CDS             complement(1303..2376)
                     /locus_tag="PK144_17835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNF67636.1"
                     /translation="MTRLLRALIALSLALVTAAAPAAPILADYRIVTGGTTGTYYAFG
                     QNLANLVARPANIALEAVSSSGSVQNVRDMRYREKVKFALVQSDVYQAYVRLAAQGDK
                     EAQDLIGPLRVVLPLYLEEIHVLVRADSPLHYVDELRGRKLYIGPDGSGAALTASTLY
                     GLMFDGARPERVDFPGATDAASGYKQALNALLDRDGRVDAVVFVAGQPVSQLVVDAEL
                     GRKYYRLLGVRAEEPAMREALKVYYPATILRDSYAWLGTDVQTLAVKAYLITYDYPQD
                     FNRRAIAAFARSLCDNYATLRAQGHPKWQQVAWSPQQPALEPLKAGWQYYSVTEPILS
                     GCSGGGNGNAACSSDRRLLGLCR"
BASE COUNT          365 a          935 c          930 g          382 t
ORIGIN      
        1 ccgatccgcc cgccgaggtc cgcccgcatg gccgacatcc tgccgttcaa gcgccccaag
       61 cctgccgacc gcatggaggc cgcggccggc cgggagcttt gcaagtccgg gtttcacaag
      121 tgggaggtgc agaaggacaa gcgcttcgac gtgaaggccg gcaggctggt cacgagctac
      181 cgctgcgcac gctgcggcgc gacccggacc gaaggccgct agccggcgcc ggccgcaacg
      241 cccggccgct gcatcggccg gcgccggcgg cgcgtatgct ggccggcccc ctgccacccc
      301 cggtttccgc catgccccgc ctgcttgcaa gcctcgtcct cgccctcggc ctgtcgctgc
      361 cggctgcggc cgccgatctc gcgaagctgc gcgcggcgtt cgcgtcggtg cgcttcatcg
      421 cctacagccc gagcgcctac accgacggcg ggctggtggg ccgggatgcg atccggcgcg
      481 atctcctgat cctggcgccg gatttcgatg gcatcgtcac ctatgcgagc gacaacggca
      541 gcgagcaggt cgcggcgctt gcacgcgaaa ccggcatccg cgccgtgatc ctcggcgtcc
      601 acaaccccgc caacaccacc gaactcgaca ccgcgatccg gctggcgcgc gaataccccg
      661 aggtcgtggt cgcgatctgc gtcggcaacg aagggctgat ctcgcaccgc tacgactggc
      721 cgacgctccg gcgcggcctc gatcacgtgc gcgcggcgct gcccgaactg ccgctgacca
      781 ccggcgagcc gttcgtgatc cacctcgaat ggacgccgcc gggcttcttc gacgcgcagg
      841 acttcatcgg cgtcaacatc catccgctgt tcgagggctg gttcacgccc gacaagctcg
      901 atgccggcat ggactatctc gacgcgatcc tcggcaaggt catcgcgcgc gcgccgggca
      961 agccggtgat gatcaaggag accggcgtgc ccggtcagcc cggcggcgaa tggtcgccgg
     1021 cggtgcaggc cggcttctgg acgcgggtcg agcgacgctt tccgcggaac ggcgaccgtg
     1081 cgttctcggc cttcgaagcc ttcgatgcgc cgtggaagcc ggcgctgatc gcgcgcgact
     1141 tcaaggacta cggcggcacg gtcgatccgc gcgaggcgta ctggggcttc tataccgtcg
     1201 acggcgaacc gaagccggtc gtcggagagc tgcgggcgca gcggcggggg ccgtgaggcc
     1261 cgaagcagga agcctcccca cgccgccccg ccgccggcgg ggtcagcggc acagcccgag
     1321 cagccgccgg tcgctgctgc acgccgcatt gccattgccg ccgccgctgc agcccgacag
     1381 gatcggttcg gtcaccgagt agtactgcca gccggccttc agcggttcca gcgccggctg
     1441 ctgcggactc caggccacct gctgccactt cggatggccc tgcgcgcgca gcgtggcgta
     1501 gttgtcgcac agcgagcgcg cgaaggccgc gatcgcgcgg cggttgaagt cctgcggata
     1561 gtcgtaggtg atcaggtagg ccttgaccgc gagcgtctgc acgtcggtgc cgagccatgc
     1621 gtagctgtcg cgcaggatcg tcgccgggta gtagaccttc agcgcctcgc gcatcgccgg
     1681 ttcctcggcg cgcacgccga gcagccggta gtacttgcgc ccgagttcgg catcgacgac
     1741 gagctgcgac accggctggc cggcgacgaa cacgacggca tcgacgcggc cgtcgcgatc
     1801 gagcagcgcg ttcagcgcct gcttgtagcc gctcgcggca tcggtggcgc cggggaagtc
     1861 gacgcgctcg ggccgggcgc cgtcgaacat cagcccgtac agcgtgctcg cggtcagcgc
     1921 cgcgccgctg ccgtcggggc cgatgtagag cttcctgccg cgcagttcat cgacgtagtg
     1981 cagcggcgag tcggcccgca ccagcacgtg gatttcctcc agatacagcg gcagcacgac
     2041 gcgcagcggc ccgatcagat cctgtgcctc cttgtcgccc tgcgcggcga ggcgcacgta
     2101 ggcctgatag acatcggact gcaccagcgc gaacttgacc ttctcgcggt agcgcatgtc
     2161 gcgcacgttc tgcaccgagc cgctggaact gacggcctcc agcgcgatgt tggccgggcg
     2221 cgcgacgagg ttcgcgaggt tctggccgaa ggcgtagtag gtgccggtcg tgccgccggt
     2281 gacgatgcgg tagtcggcga ggatcggcgc cgccggcgca gcggcggtga cgagggcgag
     2341 gctcagcgcg atgagcgcgc gcagcagacg ggtcatgcaa ggttctcccg gcaggattcg
     2401 tggcaagcgg agcatcggtc gcggggcagc cgccgcgggc atcagtcgca gagcttcagc
     2461 cgccggcgtt cgtcctcgcc ggccccgcag ggcggcagcc gggccggctg ggcggcggat
     2521 tcggcctgcc ggcgacgatc ggcctcggcg gcggcttcgt cggcgtggcg cctgcgctcg
     2581 gcttcggccg ctgctgccgc ctcggcctgc cg
//
LOCUS       DAOQIE010000428         2600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_27973_length_2600_cov_4.342633, whole genome shotgun sequence.
ACCESSION   DAOQIE010000428 DAOQIE010000000
VERSION     DAOQIE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27973_length_2600_cov_4.342633"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   40..495
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=40..68
                     /rpt_unit_seq="cggttcatccccgcgcatgcggggaattc"
     gene            complement(678..980)
                     /gene="cas2e"
                     /locus_tag="PK144_17840"
     CDS             complement(678..980)
                     /gene="cas2e"
                     /locus_tag="PK144_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HNF67637.1"
                     /translation="MLVIVLEAAPPRLRGRLALWLVEVRAGVYVGDFSKRVREMIWSQ
                     VQAGIEDGNAVMIWTSNNESGFDFVTLGPNRRLPVELDGLKLVEFLPPAEPARPSG"
     gene            complement(961..1872)
                     /gene="cas1e"
                     /locus_tag="PK144_17845"
     CDS             complement(961..1872)
                     /gene="cas1e"
                     /locus_tag="PK144_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HNF67638.1"
                     /translation="MLPPLKPLPMKERVSLVFLRYGELDVIDGAFVLVDGNGVRKQIP
                     IGAIACVMLEPGTRVSHAAAATAARVGTLLVWIGEGGVRLYASGQPGGARSDRLLYQA
                     KLALDETARLKVVRRMYALRFGEEPPQRRSVEQLRGIEGVRVRKTYQALARQYGVDWD
                     GRRYDPHDWDTSNTVNKCLSAATSCLYGISEAAVLAAGYAPAVGFIHTGKPLSFVYDV
                     ADIVKFDTVIPIAFRIAKREPANPEREVRLACRDAFRESKILGRLIPLIEDILAAGEL
                     PVPPPPEDTVPIAIPNPESIGDAGHRA"
     gene            complement(1895..2251)
                     /locus_tag="PK144_17850"
     CDS             complement(1895..2251)
                     /locus_tag="PK144_17850"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNF67639.1"
                     /translation="MWRLLANENFPRTSVLVLRASGCDVLYVAESMASVRDEAVLECS
                     RTEQRILLTFDRDYGDLLFRRRLASPPAVILLRVPMRDPARPAALVLELLMQPPGVTG
                     QFVTVGERSLRRRPLP"
     gene            complement(2251..2487)
                     /locus_tag="PK144_17855"
     CDS             complement(2251..2487)
                     /locus_tag="PK144_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNF67640.1"
                     /translation="MNWREHIVTDPEILCGKPCIKGTRLSVEFLLGVMAEGWSRTMLL
                     ENYPRLTDDDLRAVFAFAGEMLREEVIVIPQDAA"
BASE COUNT          441 a          856 c          864 g          439 t
ORIGIN      
        1 gaattccagt catcacctcg ccgggcctcg cctgccgtgc ggttcatccc cgcgcatgcg
       61 gggaattccc ggcggcctcg ctgaacggga aatagacgtg cggttcatcc ccgcgcatgc
      121 ggggaattcg ttcgttctgt aacttaaaca tcttgtacat caggttcatc cccgcgcatg
      181 cggggaattc cggcacgcgc gcggtccgtg cgcgcaggtc ggcggttcat ccccgcgcat
      241 gcggggaatt cgtcgatgcc tcttgcagat tgccggcgac ggccggttca tccccgcgca
      301 tgcggggaat tccagatcca gttcacgcca gcgcatgctg gccacggttc atccccgcgc
      361 atgcggggaa ttcgccgtcg gggctgtacg ggcgtgtcac gagggcggtt catccccgcg
      421 catgcgggga attcccggac ccgcacgcag ccagacgctg ccgagtcggt tcatccccgc
      481 gcatgcgggg aattcacttc ctccatctcc ttgatgtgca aggtaaaaaa ctcatccctg
      541 aaatccaccg ctcctgggcc ggaaccggcg gcttcggggg gcgggagggg attgattata
      601 agagcctgat tttaaaagaa gcatccgtgg gggcgggcaa tggtgctgtt cgtctgccgg
      661 cgatccaccg gaacgggtca tccggatggt ctggccggtt ctgccggcgg caggaactcg
      721 acgagcttga ggccgtcgag ttcgaccggc agccggcggt tggggccgag cgtgacgaag
      781 tcgaagccgg attcgttgtt actggtccag atcatcaccg cattgccgtc ctcgatgccg
      841 gcttgcacct ggctccagat catctcgcgc acgcgcttcg agaagtcgcc gacgtagacg
      901 ccggcgcgca cctcgaccag ccacagcgcc agccggccgc gcagtcgcgg cggtgcggct
      961 tcaagcacga tgaccagcat cgccaatcga ctcggggttg gggatggcga tcggcacggt
     1021 gtcctcgggc ggcggtggca ccggcagttc accggcggcg aggatgtcct cgatcagcgg
     1081 aatcagccgg ccgaggatct tcgattcgcg gaaggcgtcg cggcaggcca gccgcacctc
     1141 gcgttcggga ttggccggct cgcgtttggc aatacggaaa gcgatcggga tgaccgtgtc
     1201 gaatttgacg atgtcggcca cgtcgtagac gaagctgagc ggcttgccgg tatggatgaa
     1261 gccgaccgcc ggcgcgtagc cggcggcaag cacggcggct tcgctgatgc cgtacaggca
     1321 cgaggtcgcg gcactcaggc atttgttgac ggtgttcgag gtatcccagt cgtggggatc
     1381 gtaacggcga ccgtcccagt cgacgccgta ctgccttgcg agtgcctgat aggtcttgcg
     1441 cacgcgcacg ccctcgatgc cgcgcagctg ctcgacgctg cgccgctgcg gcggctcctc
     1501 gccgaagcgc agcgcataca tgcggcgcac gaccttcagg cgcgcggttt catcgagggc
     1561 gagcttcgcc tgatacagca gccggtccga gcgcgcgccg ccgggctgcc ccgaggcata
     1621 cagccgcacg ccgccttcgc cgatccagac cagcagcgtg ccgacccgcg ccgcggtcgc
     1681 cgccgcggcg tgcgagacgc gcgtgccggg ttcgagcatc acgcaggcga tcgcgccgat
     1741 cggaatctgc ttgcgcacgc cgttgccatc gaccagcacg aaggcgccgt cgatcacgtc
     1801 cagctcgccg tagcgcagga acaccagcga cacgcgttcc ttcatcggca ggggtttcag
     1861 cggcggcagc atacggatgg catcggttcc gggctcaggg cagcgggcgg cggcgcaggc
     1921 tgcgttcgcc cacggtcacg aactggccag ttacgcccgg cggctgcatc aggagctcga
     1981 gtaccagcgc cgctggccgg gcagggtcgc gcatcggtac gcgcagcagg atgacggcgg
     2041 gcggactcgc cagccggcgg cggaacaaga gatccccgta gtcccgatcg aaggtcagca
     2101 gaatgcgctg ctcggtgcgc gagcattcga gtaccgcttc gtcgcgcacg ctggccatcg
     2161 actctgcaac gtacagcaca tcgcagccgc tcgcccgcag caccaggacg ctggtgcgag
     2221 gaaaattctc gttcgccagc aggcgccaca tcaggctgca tcctgcggaa tgacgatgac
     2281 ttcctcccgt agcatttcac cggcaaaggc aaagacagcc cgcaggtcgt cgtcggtcag
     2341 ccgggggtag ttctccagca gcattgtccg ggaccagccc tcagccatta ctcccagcag
     2401 gaattcgacc gacagccgcg tgcctttgat gcagggtttg ccgcagagga tttcggggtc
     2461 ggtgacgatg tgttcgcgcc agttcatcgc agatctccgt cgtgatcagt gcaacagccg
     2521 ccggacgagc ctacacccgg cgcaccagca gcagcccgca gccgacggtg ctcccgacga
     2581 ctgatccaat gccagtgtcg
//
LOCUS       DAOQIE010000429         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_28031_length_2597_cov_5.994493, whole genome shotgun sequence.
ACCESSION   DAOQIE010000429 DAOQIE010000000
VERSION     DAOQIE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28031_length_2597_cov_5.994493"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /gene="malQ"
                     /locus_tag="PK144_17860"
     CDS             <1..1707
                     /gene="malQ"
                     /locus_tag="PK144_17860"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNF67641.1"
                     /translation="GAPGFARLAGRERGFGFAAQLYGLRRGADGGVGDFSALAEHAAA
                     LGAHGASALAISPVHALFAADPQHYSPYSPSNRQFLNYLHADAHAAGLGAEAAACDAL
                     GPSVPGTGGDFVDWPGVARARLAALRRLWDALAARLRDGDDALALDYRRFVEAGGEAL
                     ERHARFELLHGLEFGADLQRWHWRSWPAALQQPDSPEVARLAHAHAHELGFHRFAQWL
                     AARGLAAAQAAARAAGMEIGLITDIAVGTSSGGSAAWSAPGEVFTGLSIGAPPDPLGP
                     TGQTWGLVTHAPQALRSHGYAPFLQLLRAALRSAGGVRIDHILGLRRLWVVPDGENGA
                     GGCYLDYPLDDLLALVALEAHRHRALIVGEDLGTVPPGLHERLAAHGIAGMSVLWFQR
                     DHGLFVEPARWPATALAMTSTHDLPTVAGWWQGRDLDWRTQVGHLEGAELVEAVAGRA
                     CDREALWAAFTHAGIAEGPMPEALAPAPVVDAALAYTAATPAALALLPLEDLCGLGEQ
                     PNLPGTVHEHPNWCRRLPGTAEALLAEPAVAARLAMLQRLRGAGPAITAAHGEGQAPP
                     AS"
     gene            1896..>2597
                     /locus_tag="PK144_17865"
     CDS             1896..>2597
                     /locus_tag="PK144_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF67642.1"
                     /translation="MPKSTRPAAPAPLPPRATVRLQLHRDFPFAAAAAQVPYLAALGI
                     SHVYASPILKARAGSTHGYDVVDPGCVNPELGGEDGLRALVATLRAHGMGLVVDIVPN
                     HMAVGGPENPYWLDVLEWGRASPYAEFFDIDWDATDPALRGRLLAPFLGAPYGEALDR
                     GELRLHFDAVSGRFSCAYFDNRFPIAPTRYPALLRLGGEALAAAARDFRAALAGRAGG
                     RRERFDAACRRFAQEA"
BASE COUNT          281 a         1009 c          955 g          352 t
ORIGIN      
        1 ggcgcaccgg gctttgcccg gctcgccggg cgcgagcgcg gcttcggctt cgccgcccag
       61 ctctacggcc tgcgccgcgg cgcagacggc ggcgtcggcg atttctcggc cctcgccgag
      121 cacgccgccg ccctcggggc gcacggcgcc tcggcgctcg cgatcagccc ggtgcatgcg
      181 ctgttcgcgg ccgatccgca gcattacagc ccgtattcgc cgtcgaaccg ccagttcctg
      241 aactacctgc acgccgatgc gcacgccgcc ggcctcggcg ccgaggccgc ggcctgcgat
      301 gcgctcggcc cgtccgtgcc cggcaccggc ggggatttcg tcgactggcc cggggtcgcg
      361 cgcgcacggc tggcggcgct gcgccggctc tgggatgcgc tggccgcgcg ccttcgcgac
      421 ggcgatgacg cactcgcact cgattaccgc cgcttcgtcg aggccggcgg cgaggcgctc
      481 gaacgccatg cgcgcttcga gctgctgcac ggtctcgaat tcggcgccga cctgcagcgc
      541 tggcactggc gcagctggcc ggcagccctg cagcagccgg attcgcccga ggtcgcgcgg
      601 cttgcgcatg cgcatgccca tgaactcggc ttccaccgct tcgcgcagtg gctggcggcg
      661 cgcgggctgg cggcggcgca ggccgcggcg cgcgccgccg gcatggaaat cggcctgatc
      721 accgacatcg ccgtcggcac cagcagcggc ggcagcgcgg cgtggtcggc tccgggcgag
      781 gtgttcacgg ggctgtcgat cggcgcaccg cccgatccgc tcggcccgac cggccagacc
      841 tggggcctgg tcacgcatgc gccgcaggca ctgcgcagcc acggctatgc gccgttcctg
      901 cagcttttgc gcgcggcgct gcgctcggcc ggcggcgtgc gcatcgacca catcctcggg
      961 ctgcggcggc tgtgggtcgt gccggacggc gagaacggcg ccggcggctg ctacctcgac
     1021 tatccgctcg atgacctgct ggcgctggtg gcgctggagg cgcaccgcca ccgggcgctg
     1081 atcgtcggcg aggacctcgg caccgtgccg cccggcctgc acgagcgtct ggccgcgcat
     1141 gggatcgccg gcatgtcggt gctgtggttc cagcgcgacc acggcctgtt cgtcgagccc
     1201 gcccgctggc cggcaacggc gctggcgatg acctcgacgc acgacctgcc gaccgtcgcc
     1261 ggctggtggc agggcaggga cctcgactgg cgcacgcagg tcggccacct cgaaggtgcg
     1321 gagctggtcg aggcggtcgc cgggcgcgcg tgcgatcgcg aggcgctgtg ggcggcgttc
     1381 acgcacgccg gcatcgccga aggcccgatg cccgaagcgc tggccccggc gccggtcgtc
     1441 gatgccgcgc tcgcctacac cgccgcgacg ccggccgcgc tcgcgctgct gccgctcgaa
     1501 gacctctgcg gcctcggcga acagccgaac ctgccgggca cggtgcatga gcacccgaac
     1561 tggtgccggc gcctgccggg cacggccgaa gccctgctcg ccgaaccggc ggtcgccgcg
     1621 cgtctggcaa tgctgcagcg gctgcgcggg gccggccccg caatcacggc ggcgcacggc
     1681 gaggggcagg ctccgccggc gtcctgagct gcgttcccgg cgcaccgggc ttcggggccc
     1741 cgggtgaagc gtcgggcgcg ggccgggaac cgttgcccgg cgccggcgtc ggctgctgag
     1801 tgcctcgtcc gcatccgcgg gccggggcat cggaatccgc cgcgcgggcc cgggtccgcg
     1861 caccccgatc ctccccccga gcgaggcgac gcccgatgcc gaaatccacc cgccccgccg
     1921 ctccggctcc gctgccgcca cgcgcgaccg tgcgcctgca gctgcaccgg gacttcccgt
     1981 tcgcggccgc cgccgcacag gtgccgtatc tggccgcgct cggcatcagc cacgtctacg
     2041 ccagcccgat cctgaaggcg cgcgccggct cgacacacgg ctacgacgtg gtcgatcccg
     2101 gctgcgtgaa ccccgaactc ggcggcgagg acggcctgcg ggcgctggtc gcgacgctgc
     2161 gcgcgcacgg catgggcctc gtcgtcgaca tcgtgccgaa tcacatggcg gtcggcggcc
     2221 cggagaatcc gtactggctc gacgtgctcg aatggggccg cgccagcccg tatgccgagt
     2281 tcttcgacat cgactgggat gccaccgacc cggcgctgcg cggccggctg ctcgcgccct
     2341 tcctcggcgc gccctacggc gaggcgctcg atcgcggcga gctgcgcctg cacttcgatg
     2401 ccgtcagcgg gcgtttttcg tgcgcgtact tcgacaaccg cttcccgatc gcgcccaccc
     2461 gctatccggc gctgctgcgc ctcggcggcg aggcgctggc tgcggcagcg cgtgactttc
     2521 gggccgcgct ggccggccgc gccggcggcc ggcgcgagcg cttcgacgcc gcctgccggc
     2581 gcttcgcgca ggaggcc
//
LOCUS       DAOQIE010000430         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_29832_length_2505_cov_6.185306, whole genome shotgun sequence.
ACCESSION   DAOQIE010000430 DAOQIE010000000
VERSION     DAOQIE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29832_length_2505_cov_6.185306"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   17..495
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17..52
                     /rpt_unit_seq="gcttcaatgaggccgcggcattacaccgcggaaaac"
     gene            complement(763..1413)
                     /locus_tag="PK144_17870"
     CDS             complement(763..1413)
                     /locus_tag="PK144_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNF67643.1"
                     /translation="MNPRLPLLFALLGSLAAAPALAGSAVVRFVPGELNGWQVKSFQG
                     ETTYTPGRIDGRSGLHAEAKGTASGLFREQRIDLTQTPVIAWDWRVDALPQNGPDERG
                     KAGDDFAARVYVVISGGVAFWKTRTLVYVWAAREPAGAHWDNPFTGNAKMLVLRSGPK
                     DLRHWQHETRNLAADLRTAFGEDIPAVDAVAIMTDMDNSGRAAEAWYGDIEFRAAP"
     gene            complement(1410..>2505)
                     /locus_tag="PK144_17875"
     CDS             complement(1410..>2505)
                     /locus_tag="PK144_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67644.1"
                     /translation="PLAPVATLAARLDACRTASLAAAESPAALARLHAAVQVPSDYST
                     VARVLGLWPLSVLPVQALGVRYRQETLARLADPPPPLASAQRFGPLPAPPQAVGRLRR
                     DALDVPVPTAAQLDALFALHAPVFEIDGRTDADLPGRPLRRADGRLDFRPEPRVYQWP
                     SLSRLHGRPVLQLNWLIWFAARPPAAGALYAGRLDGLIWRVTLDADGRPYVYDSIHAC
                     GCYHLLFPRAGLGLTAAAAALAETPIAPYPAPAFRPGRHLRLRIASATHYLIALDEDD
                     GRPFVPYRSVDYTALYTDTDADAGTQTLFDPDGLIPGSERPERWLLWPMGVASAGAMR
                     ERGRHAIAFVGERHFDDPGLPADWLEESPP"
BASE COUNT          412 a          830 c          915 g          348 t
ORIGIN      
        1 cagctacgac ggcgaagctt caatgaggcc gcggcattac accgcggaaa accgggcaac
       61 gctgacgtgc tcgcccaact ggccgccgcg cttcaatgag gccgcggcat tacaccgcgg
      121 aaaacttcgc tgtacccgaa gggcgccacg ctgccggctg tcgtgcttca atgaggccgc
      181 ggcattacac cgcggaaaac gccacagttt cgcggcctcg gcgtgcttgg cgttgaggct
      241 tcaatgaggc cgcggcatta caccgcggaa aacacgaccg cactgcctcg ttcagcagcg
      301 tggtgagtgg cttcaatgag gccgcggcat tacaccgcgg aaaacgcgcg ggcggctgct
      361 ctctctccgg cgcatccaga ctgcttcaat gaggccgcgg cattacaccg cggaaaactt
      421 gtcgcgatcg cgcctgtatc cgtagatcgg cacgaacggg cttcaatgag gccgcggcat
      481 tacaccgcgg aaaacagacc tctcaaaaac acattccgga acaaccactt gcttctgggt
      541 ttgcgagcgc tgcgggtgag ggcgggttgc gaggtgcact tggcgctgac caggtggcgg
      601 gcggtggtta caacatcctg attataaaag aaaactccgc ttgcgagcgt tgcccgggat
      661 tttggcggca ccggagcgct cgcagcgcgg gcggcagtgt cgggtggttc gggggcgcgg
      721 acagggtgtg ccagcgcacc ggaaatgtcc agaaagccgg gattacggtg cggcccggaa
      781 ttcgatatcg ccgtaccacg cttccgccgc ccgcccggaa ttgtccatgt cggtcatgat
      841 cgccacggca tcgaccgccg ggatgtcctc gccgaaggcc gtgcgcaggt cggcggcgag
      901 gttgcgggtt tcgtgctgcc agtggcgcag gtccttcggg ccggagcgca gcacgagcat
      961 cttcgcgttg ccggtgaacg gattgtccca gtgcgcaccg gccggttcgc gcgcagccca
     1021 gacgtagacc agtgtccgcg tcttccagaa tgccacgccg cccgaaatca ccacgtacac
     1081 gcgcgccgcg aaatcatcgc cggccttgcc gcgttcatcc gggccgttct gcggcagcgc
     1141 atcgacccgc cagtcccagg cgatcaccgg cgtctgcgtc aggtcgatgc gctgctcgcg
     1201 gaacagcccc gaagccgtgc ctttcgcttc ggcgtgcagg ccgctgcggc cgtcgatgcg
     1261 gccgggggtg taggtggttt caccctggaa ggatttcacc tgccagccgt tcagctcgcc
     1321 cggcacgaag cgcacgacgg cggaaccggc gagggccggg gcggcggcaa ggctgccgag
     1381 cagggcgaac agcaggggca ggcggggatt catggcgggc tttcctccag ccagtcggcc
     1441 ggcaggccgg gatcgtcgaa atggcgttcg ccgacgaagg cgatcgcatg ccggccgcgt
     1501 tcgcgcatcg cgccggccga ggccacgccc atcggccaca gcagccagcg ctccgggcgc
     1561 tcgctgccgg gaatgagacc gtcgggatcg aacaaggtct gcgtgccggc atcggcatcg
     1621 gtgtcggtat acagggcggt gtaatcgacg ctgcgatacg gcacgaacgg gcggccgtcg
     1681 tcctcgtcga gcgcgatcag gtaatgcgtg gcgctggcga tgcgcaggcg cagatgacga
     1741 ccggggcgaa acgccggcgc cgggtacggg gcgatcggcg tttccgccag cgccgcggcg
     1801 gcagccgtca ggcccaggcc ggcgcgcggg aacagcaggt ggtagcagcc gcaggcgtgg
     1861 atcgagtcgt agacatacgg gcggccgtcg gcgtcgagcg tcacgcgcca gatcaggccg
     1921 tcgaggcggc cggcgtacag cgcacctgcg gctggcgggc gcgcggcgaa ccagatcagc
     1981 cagttcagct gcagcaccgg ccggccgtgc aggcggctca gcgacggcca ctgatagacg
     2041 cgcggctcgg gccggaagtc gaggcggccg tcggcgcggc gcagcggccg gccggggaga
     2101 tcggcatccg tgcggccgtc gatctcgaac accggcgcgt gcagcgcgaa cagcgcatcg
     2161 agctgggcgg cggtcggcac cggcacatcg agcgcatcgc ggcgcagccg gccgaccgcc
     2221 tgcggcggtg ccggcagcgg accgaagcgc tgcgcgctgg cgagcggtgg cggcggatcg
     2281 gcgaggcgcg ccagcgtttc ctgccggtag cgcaccccga gcgcctgcac cggcagcacg
     2341 ctcagcggcc acagcccgag cacgcgcgcc accgtggaat agtcggaagg cacctgcacg
     2401 gcggcgtgca ggcgcgccag cgccgccggt gactccgcgg ccgcgaggct ggcggtgcgg
     2461 caggcgtcga gccgtgccgc cagcgtcgcg acgggcgcga gcggg
//
LOCUS       DAOQIE010000431         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_30438_length_2477_cov_6.699422, whole genome shotgun sequence.
ACCESSION   DAOQIE010000431 DAOQIE010000000
VERSION     DAOQIE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30438_length_2477_cov_6.699422"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="PK144_17880"
     CDS             <1..1909
                     /locus_tag="PK144_17880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNF67645.1"
                     /translation="PAALRDGQPPLRRCEPGFRITETGRLALQEQASGRSARRRAVLA
                     TLSVADGALALSVLRERHALDGALLRTLCERGWVERLDDPQSDAPRPAVAAVTAPALH
                     DGQAAAVAAIDAAAGKYRCLLLDGVTGSGKTEVYLGAIAAVLARGRQALVLVPEIALT
                     PQLVERFRARLSGRIAVLHSGLAGAERLNAWLDARDGRADVVIGTRSAVFAPLARPGL
                     IVVDEEHDASYKQHEGFRYSARDLAVVRAHAAGVPLVLGSATPSLESLANVAAGRYTR
                     LALTHRAAAGAAAPRMELIDVRRAPLDEGLSAPLLARMRAQLDAGAQVLLFLNRRGWA
                     PVLYCCDCGWLAECSRCDARYTVHRATRRLICHHCGTEHRLPARCPKCGGTTLTGLGL
                     GTERIEQALERHFPGTGIARIDRDTTRRKGSLEAQLDAVRSGAARLLIGTQMLAKGHD
                     FPDVGLVGIVDADQGLFGADFRSAERLAQLVVQVAGRAGRAGRPGTVAIQTHHPEHPL
                     FMRLLRGDYAAIAAELLAERRAARLPPFGHQALLRAEATDAEAPAAFLTAVATLAEPL
                     LGDGHVQVHGPVPAPMERRAGRYRAQLLFEAGARAPLHALLDALAPMLGRLPQARLVR
                     WSLDVDPQDLY"
     gene            complement(1955..>2477)
                     /gene="pyrE"
                     /locus_tag="PK144_17885"
     CDS             complement(1955..>2477)
                     /gene="pyrE"
                     /locus_tag="PK144_17885"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNF67646.1"
                     /translation="NTGGALARLGRAYAQAITDAGIDYDLLFGPAYKGIPLAAATSVQ
                     LIEQYGRDVPWCFNRKEAKDHGEGGQTVGAPLAGRVLIIDDVITAGTAIRESLLLIAA
                     HGAEPAGVVIALDRQERGLGERSAIQEVEQNYGIPVISIVTLADLIDYVAGRPDFAAH
                     ETAIRAYRERYGV"
BASE COUNT          297 a          901 c          935 g          344 t
ORIGIN      
        1 gccggcggcg ctgcgtgatg gccagccacc gctgcgccgc tgcgagccgg gcttccggat
       61 caccgagacc ggccggctgg cgctgcagga gcaggcgagc ggccgctcgg cgcgccggcg
      121 cgccgtgctg gcgacgctga gtgtcgccga cggagcactg gcgctgtcgg tgctgcgcga
      181 gcggcacgcg ctcgacggcg ccctgctgcg cacgctgtgc gagcgcggct gggtcgagcg
      241 cctcgacgat ccgcagtccg acgccccccg gccggcggtc gcggcggtca ccgctccggc
      301 gctgcatgac gggcaggccg cggcggtggc cgcgatcgac gccgccgccg gaaagtatcg
      361 ctgcctgctg ctcgatggcg tcaccggcag cggcaagacc gaggtctatc tcggcgcgat
      421 cgccgcggtg ctggcgcgcg ggcgccaggc gctggtgctg gtgccggaga tcgcgctgac
      481 gccgcagctg gtcgagcgtt tccgggcgcg gctgagcggg cgcatcgcgg tgctgcactc
      541 ggggctggcc ggggccgagc ggctcaacgc ctggctggat gcgcgcgacg gccgcgccga
      601 tgtggtcatc ggcacgcgct cggcggtgtt cgcaccgctg gcgcgcccgg ggctgatcgt
      661 cgtcgacgag gagcacgacg cttcgtacaa gcagcacgag ggcttccgct attcggcgcg
      721 cgatctggcc gtcgtgcgcg cacacgccgc cggcgtgccg ctggtgctcg gctcggcgac
      781 gccgtcgctg gagagcctcg ccaacgtcgc cgccggccgc tacacgcgcc tggcgctgac
      841 gcatcgcgcc gcggccggcg cggcggcacc gcgcatggag ctgatcgatg tgcgccgcgc
      901 cccgctcgac gaggggctgt cggcgccgct gctcgcgcgc atgcgcgcgc agctcgatgc
      961 cggcgcgcag gtgctgctgt tcctgaaccg ccgcggctgg gcgccggtgc tgtactgctg
     1021 cgactgcggc tggctggccg aatgctcacg ctgcgatgcg cgctataccg tgcaccgcgc
     1081 cacgcgccgg ctgatctgcc accactgcgg aaccgagcac cggctgccgg cgcgctgccc
     1141 gaagtgcggc ggcacgacgc tgaccgggct cgggctcggc accgagcgca tcgaacaggc
     1201 gctggagcgg catttccccg gcaccggcat cgcgcgcatc gaccgcgaca cgacgcggcg
     1261 caagggcagc ctcgaagcgc agctcgacgc cgtgcgcagc ggcgcggcgc ggctgctgat
     1321 cggcacacag atgctcgcca agggccatga cttcccggac gtgggcctgg tcggcatcgt
     1381 cgatgccgac cagggcctgt tcggggccga cttccgctcg gccgagcggc tggcgcagct
     1441 cgtcgtgcag gtcgccggcc gtgccggtcg cgccggccgg ccgggcacgg tggcgatcca
     1501 gacccatcac cccgagcatc cgctgttcat gcggctgttg cgcggcgatt acgcggcgat
     1561 cgccgccgaa ctgctggccg agcgccgcgc cgcgcggctg ccgccgttcg gccaccaggc
     1621 gctgctgcgc gccgaggcga ccgatgccga agcgccggcg gccttcctga cggcggtcgc
     1681 gacgctcgcc gaacccttgc tcggcgacgg ccacgtgcag gtccacggtc cggtgccggc
     1741 gccgatggag cgccgcgccg ggcgctatcg cgcgcaactg ctgttcgagg ccggcgcgcg
     1801 cgcgccgctg cacgcgctgc tcgatgcgct ggccccgatg ctcggccggc tgccgcaggc
     1861 acggctggtg cgctggtcgc tcgatgtcga tccgcaggat ctgtattgaa cacgcctgaa
     1921 cacgcctgaa ccggcctgaa cggaccgcgc ggcttcagac gccgtaacgc tcgcggtagg
     1981 cgcggatcgc ggtttcgtgc gcggcgaaat ccgggcggcc ggcgacatag tcgatcaggt
     2041 cggcgagcgt gacgatgctg atgaccggga tgccgtagtt ctgctcgacc tcctggatcg
     2101 ccgaacgctc gccgaggccg cgctcctgcc gatccagcgc gatgaccacg ccggccggtt
     2161 cggcgccgtg cgcggcgatc agcagcagcg attcgcggat cgcggtgccg gcggtgatca
     2221 cgtcatcgat gatcagcacg cgcccggcga gcggcgcacc gacggtctgg ccgccctcgc
     2281 cgtgatcctt ggcttccttg cggttgaagc accacggaac gtcgcggccg tactgttcga
     2341 tgagctgcac cgaggttgcc gcggccagcg ggatgccctt gtaggccggg ccgaacagca
     2401 ggtcgtagtc gatgccggca tcagtgatcg cctgcgcgta ggcccggccg agccgggcca
     2461 gcgcgccacc ggtgttg
//
LOCUS       DAOQIE010000432         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_30561_length_2471_cov_8.221026, whole genome shotgun sequence.
ACCESSION   DAOQIE010000432 DAOQIE010000000
VERSION     DAOQIE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30561_length_2471_cov_8.221026"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..2184)
                     /gene="htpG"
                     /locus_tag="PK144_17890"
     CDS             complement(280..2184)
                     /gene="htpG"
                     /locus_tag="PK144_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNF67647.1"
                     /translation="MTVDTHKETLGFQTEVKQLLHLMIHSLYSNREIFLRELISNASD
                     ACDKLRFEALTDGALFEDDATLAIHIAADKDARTLTIRDNGIGMSRQEVIDNIGTIAR
                     SGTRAFFEQLSGDQQKDASLIGQFGVGFYSAFIVAERVTLTTRRAGLTAGHGVRWESA
                     GEGDYTLESIEQAARGTEVTLHLREEAAEFLDDWRLRSIITKYSDHIQLPVMLTVSKP
                     AEEEGGEATREIEQVNKASALWTRAKGDISDDDYKAFYKHVGHDFQDPLAWTHNRVEG
                     KYEYTSLLYVPGRAPFDLWDRDQKHGVKLYVQRVFIMDDAEHLMPRYLRFVRGVIDSN
                     DLPLNVSRELLQSNKVIDSIRSGSVKKVLGLLEEIAKNEPEKYATFWQEFGRVLKEGP
                     GEDYANREQIAKLLRFATTQADTPEQSVSLADYVARMKEGQTAIYYITADSFAAAKNS
                     PHLEVFRKKGIEVLLMSERVDEWLMSHLEEFDGKRLVSVAKGALDLGAIESDEEKAQQ
                     KEVEERFKGLVERVKTVLGDAVKDVRLSQRLTDSPACLVVEEYAMSAHLERLLKEAGQ
                     DVPGSKPTLELNPEHPLIARLDAEAEDTRFADLAHLLFGQATLAEGGTLEDPATFVKR
                     LNGLLLTLAA"
BASE COUNT          365 a          815 c          827 g          464 t
ORIGIN      
        1 tctgctgccg ctcggcagcc atcagggtat tgcaatcgtc gaagccgccg aagcgctccg
       61 gcagatcacc gagacctgat cgcagcccct gactgcaagc cccggcgtcc gggagggatg
      121 cctgcgcgcc agcggctgat ccctgcgcgg catggacggg gccttgcgcg tcatcggcca
      181 atccttgcgc agcagacccg gacgcccctg aaacagccgc ggcccgcccg gtttcccgga
      241 cgggctgcgg tttctgccgg acgctcgatg cgggccggtt caggccgcca gcgtcagcag
      301 caggccgttc aggcgcttga cgaaggtcgc cgggtcttcg agcgtgccgc cttcagcgag
      361 cgtcgcctga ccgaacagca ggtgcgcgag gtcggcgaag cgcgtgtcct cggcctcggc
      421 atcgaggcgc gcgatcagcg ggtgctcggg gttcagctcc agcgtcggct tgctgccggg
      481 cacgtcctga ccggcttcct tcagcagccg ctccagatgc gcgctcatcg cgtattcctc
      541 gacgacgagg caggccggcg aatcggtcag gcgctgcgac aggcgcacgt ccttgaccgc
      601 gtcaccgagc acggtcttca cgcgctcgac caggcccttg aagcgctcct cgacttcctt
      661 ctgctgcgcc ttttcctcgt cggattcgat cgcgccgaga tcgagcgcac ccttggcgac
      721 cgacaccaga cgcttgccgt cgaattcttc gaggtgcgac atcagccact cgtcgacgcg
      781 ctccgacatc agcaacacct cgatgccctt cttgcggaac acttcgaggt gcgggctgtt
      841 cttcgcggcg gcgaaggaat cggcggtgat gtagtagatc gccgtctggc cttccttcat
      901 gcgcgcgacg taatcggcga gcgagacgct ctgctccggc gtgtcggcct gggtcgtggc
      961 gaagcgcagc agcttcgcaa tctgctcgcg gttggcgtag tcctcgcccg ggccttcctt
     1021 cagcacgcga ccgaattcct gccagaaggt cgcgtacttt tcgggttcgt tcttcgcgat
     1081 ctcttcaagc aggccgagca ccttcttgac cgagccggag cggatcgagt cgatgacctt
     1141 gttcgactgc agcagctcgc gcgagacgtt cagcggcagg tcgttggaat cgatgacgcc
     1201 gcgcacgaag cgcaggtagc gcggcatcag gtgttcggcg tcatccatga tgaacacgcg
     1261 ctgcacgtac agcttcacgc cgtgcttctg gtcgcggtcc cacaggtcga acggcgcacg
     1321 cccgggcacg tacagcagcg aggtgtactc gtacttgccc tcgacgcggt tgtgggtcca
     1381 ggcgagcggg tcctggaagt cgtggccgac gtgcttgtag aaggccttgt agtcgtcgtc
     1441 gctgatgtcg cccttcgcgc gcgtccacag tgccgaggcc ttgttgacct gctcgatctc
     1501 gcgggtcgcc tcgccgcctt cctcctcggc cggcttgctg acggtcagca tcaccggcag
     1561 ctggatgtga tccgagtact tggtgatgat cgagcgcagc cgccagtcgt cgaggaactc
     1621 ggcggcttcc tcgcgcaggt gcagcgtcac ttcggtgccg cgcgcggcct gctcgatgga
     1681 ttcgagcgtg tagtcgccct cgccggccga ttcccagcgc acgccgtgtc cggcggtcag
     1741 gccggcgcgg cgcgtggtca gcgtcacgcg ctcggcgacg atgaacgccg aatagaagcc
     1801 gacgccgaac tggccgatca gcgaggcgtc cttctgctgg tcgccggaga gctgctcgaa
     1861 gaaggcgcgc gtgccggaac gggcgatcgt gccgatgttg tcgatgacct cctgacggct
     1921 catgccgatg ccgttgtcgc ggatggtcag cgtgcgcgcg tccttgtcgg cggcgatgtg
     1981 gatggcgagc gtcgcgtcgt cctcgaacag cgcaccatcg gtcagggcct cgaagcgcag
     2041 cttgtcgcag gcatcggacg cgttggagat cagctcgcgc aggaagatct cccggttcga
     2101 atacagcgag tggatcatca ggtgaaggag ctgcttcacc tcggtctgga agcccagcgt
     2161 ttccttgtgc gtatcgacgg tcatggtcgg atcggtctcc ggctcggtgg atgagggaac
     2221 gcggccggac atgcgggcac cggccggcgg tttcaagtcc cccggctgca tcggggcgcc
     2281 acggcggttt gcggcgtctg cgcggacgcc tccgctggcc gcacatgcgg catttgccgg
     2341 ttgtggccga ccgccggagc acggaacctg accgtaccgt cacttgacgc tccggcggac
     2401 ggcagccagc ttgatcgcca attccgagcg attttcacgg ttactcgggt caggcacgga
     2461 ttcatgccgc t
//
LOCUS       DAOQIE010000433         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_30715_length_2463_cov_8.084718, whole genome shotgun sequence.
ACCESSION   DAOQIE010000433 DAOQIE010000000
VERSION     DAOQIE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30715_length_2463_cov_8.084718"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1290)
                     /locus_tag="PK144_17895"
     CDS             complement(19..1290)
                     /locus_tag="PK144_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HNF67648.1"
                     /translation="MRLTRRGFVGAAALAGFAALSRRLPAAPPARVLVVGGGYAGACC
                     AKYLQRLDPALAVTLLTRPDPYYTCPFSNLLLAGRLGLDELEQRHDALAHAGVEVRYG
                     EAIAVDPVAKRVQLDDGSALVADRLVLAPGVELAFDALPGYDAAAAAILPHAWEAGAQ
                     TVLLRRQLEALPDGGTVLISVPPEPYRCPPGPYERASLIAWWLRRARPRAKLLILDAK
                     DRFSKQALFREGWQALYGQQLEWVPGSDGGRIERVEAATRTLHAGFGFGPYRGDVVNV
                     IPPQRAPKLLREAGLTDASGWCPVSPLAFESSRHPGVHVLGDAADAAPMPKSAYSATS
                     QARAVAAAIVAQLRGEVPAAPVYLNTCYSLLAPDYGISIAAVYRVADGRLTAVPEAGG
                     TSPLAADAAFRAREAVFAAGTWTSLNADAFG"
     gene            complement(1290..1574)
                     /locus_tag="PK144_17900"
     CDS             complement(1290..1574)
                     /locus_tag="PK144_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNF67649.1"
                     /translation="MLAALLLLAPVRLPAEAPGRGFGLAAACFGCHGTDGVSRGAIPS
                     LAGRSERFLLLALGEYRSGASSGTVMNRIARGYSDEELALLAAWFAGRRE"
     gene            complement(1617..2108)
                     /locus_tag="PK144_17905"
     CDS             complement(1617..2108)
                     /locus_tag="PK144_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNF67650.1"
                     /translation="MRSVLRAGLALAIAAAVLPVHAHEYLLKGMHIEHPWARPTPPNA
                     QNGAVYLTLHEHGDGSDKLVSASTPVAARAELHTHQMDGGVMKMRPVESIAIEQGTVT
                     QLKPGGLHVMLMDLKKPLEGGAKFPLTLKFERAGEVTVEVDVQPNPPAPGDHGHEHGA
                     TTE"
     gene            complement(2203..>2463)
                     /gene="ssb"
                     /locus_tag="PK144_17910"
     CDS             complement(2203..>2463)
                     /gene="ssb"
                     /locus_tag="PK144_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF67651.1"
                     /translation="SQVYIEGSLRTRKWQDKEGRDQYTTEIVASDMQMLGGKSGGGSA
                     PFERDGGEMPAAPRASGRPAPAAQPPAASGGFDDNLDDDIPF"
BASE COUNT          348 a          869 c          916 g          330 t
ORIGIN      
        1 acgcagcgga aaaccgcttc agccgaaggc gtcggcgttc aggctggtcc aggtgccggc
       61 ggcaaagacg gcctcgcggg cgcggaacgc ggcatcggcc gccagcggac tggtgccgcc
      121 ggcctcgggc acggccgtga gccggccgtc ggcgacgcgg tagacggcgg cgatcgagat
      181 gccgtaatcg ggcgcgagca ggctgtagca ggtgttcagg tagaccggcg ccgccggcac
      241 ctcgccacgc agttgcgcga cgatcgccgc ggcgacggcg cgcgcctgac tcgtggccga
      301 atacgccgat ttcggcatcg gcgcggcatc ggcggcatcg ccgagcacgt gcacgcccgg
      361 gtgccgggat gactcgaacg cgagcggcga gaccgggcac cagccggagg catccgtgag
      421 cccggcctcg cgcagcagct tcggcgcgcg ctgcggcggg atgacgttga ccacatcgcc
      481 gcggtacggc ccgaagccga agccggcgtg cagcgtgcgc gtcgcggcct cgacccgctc
      541 gatgcgtccg ccgtccgagc ccggcaccca ttccagctgc tggccgtaca gggcctgcca
      601 gccttcgcga aacagcgcct gcttcgagaa tctgtccttg gcatcaagga tcaggagctt
      661 cgcgcgcggc cgggcgcgcc tgagccacca cgcgatcagg ctggcgcgct cgtacggccc
      721 gggcgggcag cgatagggct cgggcggcac gctgatcagc accgtgccgc cgtcgggcag
      781 ggcttcgagc tggcggcgca gcagcacggt ctgggcgccg gcctcccagg catgcggcag
      841 gattgcggcg gccgcggcgt cgtagccggg cagcgcatcg aacgcgagct cgacgccggg
      901 ggcgagcacg agccgatcgg cgaccagggc gctgccatcg tcgagctgca cgcgcttcgc
      961 gaccggatcg acggcgatcg cctcgccgta acggacttcg acgccggcat gcgcaagcgc
     1021 atcgtgccgc tgttcgagtt cgtcgagccc gagccgcccg gccagcagca gattgctgaa
     1081 cgggcaggtg taataggggt cggggcgggt cagcagcgtc acggcgagcg ccggatcgag
     1141 gcgctgcagg tacttcgcgc agcaggcgcc ggcgtaaccg ccgccgacca ccagcacgcg
     1201 cgccggcggt gctgccggca ggcgccggct cagcgccgcg aaaccggcca gcgccgccgc
     1261 gccgacgaaa ccgcgccgcg tcaggcgcat cactcgcgcc ggcccgcgaa ccaggcggcg
     1321 agcagcgcga gttcctcgtc gctgtagccg cgggcgatgc ggttcatgac cgtgccgctg
     1381 ctggcaccgg acctgtattc acccagcgcc agcagcagga agcgctcgct cctgccggca
     1441 agggacggga tggcgccgcg gctgacgccg tccgtgccgt ggcagccgaa gcaggcggcg
     1501 gcgagcccga aaccgcgccc gggcgcctcg gccggcaacc ggaccggggc gaggagcagc
     1561 agggcggcca gcatgccgcg cgccagcctg caagccacgg tgcgccggcg ctccctttac
     1621 tcggtggtgg cgccgtgctc gtgcccgtga tcgccgggag ccggcggatt cggctgcacg
     1681 tcgacctcga cggtgacctc gccggcgcgc tcgaacttca gcgtcagcgg gaatttcgca
     1741 ccgccttcca gcggcttctt caggtccatc agcatcacgt gcaggccgcc gggcttgagc
     1801 tgcgtgaccg tgccctgctc gatcgcaatc gactcgaccg gccgcatctt catgacgccg
     1861 ccgtccatct ggtgcgtgtg cagttcggcc cgggccgcga ccggcgtgct ggcgctgacg
     1921 agcttgtcgc tgccatcgcc atgttcgtgc agggtcagat acacggcacc gttctgtgca
     1981 ttcggcgggg tcggtcgcgc ccacggatgc tcgatgtgca tgcccttgag caggtattca
     2041 tgggcatgca ccggcagcac ggcggcagcg atggcgaggg caagccctgc gcgcaggacg
     2101 gatctcatga ggcactccgg gagggtttct gggggacgcc gagtgtagac gcccgctggc
     2161 ggcgcggggc cggatgcgac gacgggctgc aagccgccgg gttcagaacg ggatgtcgtc
     2221 gtcgaggttg tcgtcgaaac cgccggaggc cgccggcggc tgcgcggccg gggccggacg
     2281 gccggaagca cggggcgccg ccggcatctc gccgccgtcg cgctcgaacg gcgcgctgcc
     2341 gccgccggac ttgccgccga gcatctgcat gtcgctggcg acgatctcgg tcgtgtactg
     2401 atcgcgccct tccttgtcct gccacttgcg cgtgcgcagg ctgccctcga tgtagacctg
     2461 cga
//
LOCUS       DAOQIE010000434         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_30885_length_2455_cov_5.452500, whole genome shotgun sequence.
ACCESSION   DAOQIE010000434 DAOQIE010000000
VERSION     DAOQIE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30885_length_2455_cov_5.452500"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="cas6e"
                     /locus_tag="PK144_17915"
     CDS             complement(<1..639)
                     /gene="cas6e"
                     /locus_tag="PK144_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HNF67652.1"
                     /translation="MNQDDFLFSHIELRTERCDSLFMRRLLAGGGYGLHQAIWRLLPC
                     DPEARRDFLYHRIDGAGVKFYVVSARELVDDTGLWRIRSKPYAPQIEAGESLNFTLRA
                     NPTVARGREPGGKGKSIRHDVVMDARRSFPEASEAERVAQAGMHWLHGREGQRAERHG
                     YVIEAAHADGYTQHRLHKPGTERAITFSTLDYVGRLRVTDPERFRSALFGGIG"
     gene            complement(636..1385)
                     /gene="cas5e"
                     /locus_tag="PK144_17920"
     CDS             complement(636..1385)
                     /gene="cas5e"
                     /locus_tag="PK144_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HNF67653.1"
                     /translation="MDFLVFRLYAPLAAWGGIAVGQERASDACPGKSAIVGLLGAALG
                     IRRHEEARLVALDAGYGYGVRIDAEGVVLRDYHTAQVPSRVDLKGRPHCTRRDELAPA
                     RATLHTILSFREYRCDALATVALWARSEAPCPLADLAAALRRPRFVLSLGRKSCAPGL
                     PLEPQIIVADHFDTALDAARFAGEQWLGSDDDPLGLQRNDLCLAWDVDAPVSRAAQMV
                     VRRRDEIRSRRRWQFGERSEAQCRLARESGA"
     gene            complement(1390..2454)
                     /gene="cas7e"
                     /locus_tag="PK144_17925"
     CDS             complement(1390..2454)
                     /gene="cas7e"
                     /locus_tag="PK144_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HNF67654.1"
                     /translation="MTHYGPSNLNRDDSGRPKTVVMGGTPRLRVSSQSLKRAWRTSDV
                     FAAAFGRPAGLGETGEGTHLGTRTKRMGVEIYDTLLGAGLAEKLAQECAASIAGRFGK
                     LKKADAEKARSGLEIEQLAHFSPEERQAITDLLPRLITEKRAPTEAETALLRQPRAAV
                     DIALFGRMLADSPAYNIEAAAQVAHAVAVHRCTIEDDYFTAVDDLNRHDEDAGAGHVG
                     EAEFGAAVFYLYLCVDRELLCENLGGDRALTDRALAALVEAATKVAPTGKQNSFASRA
                     RASYALLEKGDEQPRQLSVAFLKPVSGSDPLADAIDLLRATRAKMDQVYGPCAAAVAE
                     LDALAGTGNLAELQAFAAGD"
BASE COUNT          376 a          820 c          874 g          385 t
ORIGIN      
        1 cccgatgccg ccaaacagcg ccgaacggaa gcgctcggga tcggtcacgc gcaaccggcc
       61 gacgtaatcg agcgtgctga aggtgatggc ccgttcggtg ccgggtttgt gcaagcggtg
      121 ctgggtgtag ccatcggcat gggccgcttc gatgacgtag ccgtgacgct cggcccgctg
      181 gccctcgcgg ccgtgcagcc agtgcatgcc ggcctgcgcg acgcgctcgg cttcgctcgc
      241 ctcggggaag ctgcggcgtg catccatcac cacgtcatgg cggatggatt ttcccttgcc
      301 gccgggctcg cgcccgcggg cgaccgtcgg gttggcgcgc agcgtgaagt tcagcgactc
      361 gcccgcctcg atctgcggcg catagggctt gctgcggatg cgccacaggc cggtgtcatc
      421 aacgagttcg cgtgccgaga cgacgtagaa cttcacgccg gcgccgtcga tgcggtgata
      481 caggaagtcg cgccgcgcct ccggatcgca gggcagcaag cgccagatcg cctgatgcag
      541 gccgtaaccc ccgccggcga gcagtctgcg catgaatagc gaatcgcagc gctcggtccg
      601 cagttcgatg tggctgaaca gaaaatcgtc ctgattcatg ctcctgactc ccgggcaagc
      661 ctgcactggg cttccgatcg ttcaccgaac tgccagcgac ggcggctgcg gatttcgtcg
      721 cgacgccgga cgaccatctg cgcggcgcgg ctgaccggag catcgacatc ccaggccagg
      781 cacaggtcgt tgcgctggag gccgagcgga tcgtcatcgc tgcccagcca ctgctcaccg
      841 gcgaagcggg cggcatcgag cgcggtatcg aagtgatcgg ccacgatgat ctgcggctcc
      901 agcggcaggc ccggggcgca ggacttgcgc ccgaggctca gcacgaagcg cggccggcgc
      961 agcgcggcgg ccagatcggc gagcgggcag ggcgcttcgc tgcgtgccca cagcgcgacc
     1021 gtggccagtg catcgcagcg gtactcgcga aacgacagga tggtgtggag cgtggcccgc
     1081 gccggggcga gttcgtcgcg ccgggtgcaa tgcgggcgcc ctttcagatc gacgcgcgag
     1141 ggcacctgcg cggtgtggta atcgcgcagc acgacccctt cggcgtcgat gcgcacgcca
     1201 tagccgtagc cggcgtcgag cgcgaccagc cgcgcttcct cgtggcggcg gatgccgagg
     1261 gcggccccga gcaggccgac gatcgccgac ttgccggggc aggcatcgga cgcccgctcc
     1321 tggccgacgg cgatcccgcc ccaggccgca agcggcgcgt aaagacgaaa tacgaggaag
     1381 tccatagcct cagtctccgg ccgcaaacgc ctgcagctcg gcaagattgc cggtgccggc
     1441 gagcgcatcg agttcggcga cggccgccgc acacggaccg tagacctgat ccatcttcgc
     1501 gcgggttgca cgcagcaggt cgatggcatc ggcgagcgga tcgctgccgc tgaccggttt
     1561 caggaaggcg accgacagct ggcgcggctg ctcgtcgcct ttctccagca aggcgtagga
     1621 ggcacgggcg cgggaggcga agctgttctg cttgccggtc ggcgcgacct tggtcgcggc
     1681 ttcgaccagc gcggccaggg cccggtcggt gagcgcgcgg tcgccgccca ggttctcgca
     1741 cagcagttcg cgatccacgc acaggtacag ataaaacacc gcggccccga attcggcttc
     1801 gccgacatgg ccggcgccgg cgtcctcgtc gtggcggttc agatcgtcga cggccgtgaa
     1861 atagtcgtcc tcgatcgtgc atctgtgcac ggcaacggcg tgcgcgacct gggcggcggc
     1921 ttcgatgttg taagcgggtg aatcggcgag catgcggccg aacagcgcaa tgtcgacggc
     1981 ggcacgcggc tgacgcagca aggcggtttc ggcttcggtc ggcgcgcgct tttccgtgat
     2041 cagcctcggc agcagatcgg tgatcgcctg gcgttcctcc gggctgaaat gcgcgagctg
     2101 ctcgatctcc agccccgaac gcgccttttc ggcatcggcc ttcttcagct tgccgaagcg
     2161 cccggcgatg ctggccgcgc attcctgcgc gagcttttcg gcgaggccgg caccgagcag
     2221 ggtgtcgtag atttcgacgc ccatgcgctt ggtgcgggtg ccgagatggg tgccttcgcc
     2281 ggtttcaccc agtccggccg gacggccgaa ggccgcggcg aacacatccg aggtgcgcca
     2341 cgcacgcttg aggctttgcg aggacacgcg caggcgcggc gtgccgccca tcaccacggt
     2401 tttcgggcgg ccggaatcgt cgcggttcag gttcgacggg ccgtagtggg tcagc
//
LOCUS       DAOQIE010000435         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_31619_length_2420_cov_8.515433, whole genome shotgun sequence.
ACCESSION   DAOQIE010000435 DAOQIE010000000
VERSION     DAOQIE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31619_length_2420_cov_8.515433"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..963
                     /locus_tag="PK144_17930"
     CDS             73..963
                     /locus_tag="PK144_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNF67655.1"
                     /translation="MNRRPPPPAVTELDPDGLRLSLRERLEFIELRLYWEGRINRGDI
                     AARFGVTLQVATADLNRYQALAPAAIEYDRNAKAYYAAAGFEPRLIRPDAQGWLMQLQ
                     ALGSGLIAPAQGWLGWQPEWATHAQPQRLVAAATLRQVLVAIRERRALHVCYQSMSRP
                     EPHWRWLSPHALAHDGWRWHARAYCHEHGDYRDFVLARFLDLEAPAGDAQGSAPEDID
                     WQTRVRLRIAANPALSEAQRRIIELDYGMTNGSAEIICRRSMLYYTRRQLGLVEGEAA
                     VAADRQLILLEASEEAPGTS"
     gene            complement(1255..2277)
                     /locus_tag="PK144_17935"
     CDS             complement(1255..2277)
                     /locus_tag="PK144_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNF67656.1"
                     /translation="MRLSRRTSVLALLAGLIASSAYAAEKLPSFKGDINQTSVSGLSS
                     GAFMAAQLQVAYSATITGAGIVAGGPYYCAAGLIANTSICMGQVPFVAPNPALMVSAA
                     QGFAALGQIDSLDNLKNDKVYIFSGTDDSVVKQVAVDATASFFAYAGVPAENLLYVKT
                     VPAGHAFITPSFGNDCSANAGPYISHCIKDGTGYDQAGAILQHIYGTLKAPVTTLSGK
                     IVEFDQRPFAPAISSMAPTGYLYVPKSCADGAACKVHVAIHGCLQSAAAVGDDVYSRT
                     GYNNWADSNDIVVLYPQVNVSYTIPFNPQGCWDWWGYTGSNYALKSGVQMEAIWTMVQ
                     TLVAKP"
BASE COUNT          429 a          767 c          814 g          410 t
ORIGIN      
        1 gcgacaacgg ctgcaatcct aatccatcaa gtcaaaaatt ccatctgttt tttttactga
       61 tatattttac atatgaacag acgccccccg ccccccgctg ttaccgaact cgatcccgac
      121 gggctgcgcc tgtcgctgcg cgagcggctc gaattcatcg agctgcgcct gtactgggaa
      181 ggccgcatca accgcggcga catcgcggcg cgcttcggcg tgacgctgca ggtggcgacg
      241 gccgatctga accgctatca ggcgctggcg cccgcggcga tcgagtacga ccgcaacgcc
      301 aaggcgtact acgccgcggc cggcttcgag ccgcggctga tccggcccga tgcgcagggc
      361 tggctgatgc agctgcaggc gctcggcagc ggtctgatcg cgccggccca gggctggctc
      421 ggctggcagc cggagtgggc cacgcatgcg cagccgcagc gtctggtggc ggcggccacg
      481 ctgcgtcagg tgctggtcgc gatccgcgag cggcgggccc tgcacgtgtg ctaccagtcg
      541 atgtcccgcc ccgaaccgca ctggcgctgg ctgagcccgc acgcgctcgc gcatgacggc
      601 tggcgctggc atgcgcgggc gtactgccat gagcacggcg attaccggga tttcgtgctc
      661 gcgcgctttc tcgacctcga ggcgccggcg ggcgatgcgc agggcagtgc gccggaagac
      721 atcgactggc agacccgggt aaggctgcgc atcgctgcca atccggccct gagcgaggcg
      781 cagcgccgga tcatcgagct ggactacggc atgacgaatg gatcggcgga aatcatctgc
      841 cggcgctcga tgctctatta cacgcgccgg cagctcggtc tcgtggaggg cgaggccgca
      901 gtcgctgcgg atcggcagct gatcctgctg gaggcctctg aggaagcgcc gggcacttcc
      961 tgaatcgtat ctgccacagg gcgcgaaggg ctgataacac tgatcatacc gatcacacgt
     1021 gaatgcatgg cttttctccc ccgctcccca actcctctcc cacaaggggc gaggggctga
     1081 aagacaaggc gttccggtgt gaccggcatc acggtccgca ggaatcgcag cgggcaggaa
     1141 actggcagcg gtgtgcagtg gcactcatga aaaacgccgg attcgcatgc gaatccggcg
     1201 tccgaggtca tttccgtgcc ggtacgggcc ggcaggatgt ggccggcaaa agaatcaggg
     1261 tttcgcgacc agcgtctgca ccatcgtcca gatcgcctcc atctgcacgc cggatttcag
     1321 tgcgtaattc gagccggtgt aaccccacca gtcccagcag ccctgcggat tgaacgggat
     1381 cgtgtaggag acattgacct gcggatacag cacgacgatg tcattgctgt ccgcccagtt
     1441 gttgtaaccg gtcctgctgt agacatcgtc gccgaccgcg gcagccgact gcaggcagcc
     1501 gtgaatggcg acatgcacct tgcaggccgc gccgtcggcg caggatttcg gcacgtacag
     1561 gtagccggtc ggcgccatgc tgctgatcgc cggcgcaaac ggccgctggt cgaattcgac
     1621 gatcttgccg ctcagcgtcg tcaccggcgc cttcagcgtg ccgtagatgt gctgcaggat
     1681 cgcgccggcc tggtcgtaac cggtgccgtc cttgatgcag tggctgatgt acgggccggc
     1741 gttggcgctg cagtcgttgc cgaaggacgg cgtgatgaag gcgtggccgg ccggcacggt
     1801 cttcacgtac agcaggttct cggccggcac gccggcatag gcgaagaacg aggccgtggc
     1861 atcgacggcg acctgcttga cgacgctgtc gtcggtgccg ctgaagatgt agaccttgtc
     1921 gttcttcagg ttgtcgaggc tgtcgatctg gccgagcgcg gcaaagccct gtgcggccga
     1981 taccatcagc gccggattcg gcgcgacgaa cggtacctgt cccatgcaga tcgaggtatt
     2041 cgcaatcagc ccggccgcgc agtaatacgg accgccggcg acgatgccgg cgccggtgat
     2101 cgtggccgaa tacgcgacct gcagctgcgc agccatgaac gcaccggacg acaggccgga
     2161 caccgaggtc tgattgatgt cgcccttgaa ggacggcagt ttctcggcgg cgtaagcaga
     2221 acttgcgatc aggccggcaa gcagggccag aacggacgtg cgacgggaca ggcgcatgaa
     2281 agctccttgc aggacgtgcg ggaggtggaa acaggcgtgc gaagtcgcgg cagcgggcgc
     2341 gcggctgttt cgccgcgtca ccggtaaagg ccgcccggtg cttataaccc gcgtgcaagc
     2401 gcagtcgcca ccctcgaaac
//
LOCUS       DAOQIE010000436         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_32047_length_2402_cov_5.034512, whole genome shotgun sequence.
ACCESSION   DAOQIE010000436 DAOQIE010000000
VERSION     DAOQIE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32047_length_2402_cov_5.034512"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2325
                     /locus_tag="PK144_17940"
     CDS             <1..2325
                     /locus_tag="PK144_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNF67657.1"
                     /translation="LQILRPGHPQNYYDEFSLCPNEALRQKLNGIELLIENWHTLMPA
                     RPPERSVVRKGAENDEAYVRRVLGPLAASRNLIVLNDEAHHAYRRPAELKISRKDVES
                     LGIDLEEATCWIAGLDRIHRQRRIVRCFDLSATPFAPTGKTSTEAALFDWVVSDFGLN
                     DAIEAGLVKTPRVVIRDDALPDARTYRSRLYHLYRETEVGEDINRRGAQATEALPQLV
                     RIAYTLLGADWRATRQSWQAAGHDIPPVMLTVCNRTETAARIEHYFGSGDVQWPELQA
                     PERTLRVDSKVLAKAEAGEAAGSDKAYEERLQAIVAATDIPATRKAELRALKKEELLR
                     AIVDNVGKRGTGGQDLQNVISVAMLSEGWDAKNVTHILGLRAFTSQLLCEQVVGRGLR
                     RVAYETETVRCPDGVERELFRPEYVNVFGVPLTVFQDSEEGGDPPPPPRPSTAVQSLA
                     ERSDFEIVWPNVLRIDAVVRPELTIDWAQVPALKIDPARIQLSAELAPALGDAADLSR
                     AVSIDLEKLPADFRLQRLLFVAARKAFEQLRPGYEGSREWLVQQLIRLVERFFASDRL
                     EIPSRFHQDPLRRRILIALSIDEIVQHVIGQIEQRNVERLEPVFDPQWPIGSTRQMRT
                     WYTTRPCLETAKSQISHAVADSTWEQYTVGRLEACAGVAAFARNDHLGFQVHYLWHGS
                     RRRYVPDFLIRFANGKQMVLEIKGEDSEQDRCKRRALDAWVQAVNSRGGFGVWCRDVV
                     CGEPARIDDVLDHHAGTGGASMPVGEAQFSLGTG"
BASE COUNT          418 a          813 c          812 g          359 t
ORIGIN      
        1 ctgcagatcc tgcggccggg gcatccgcag aactattacg acgagttctc gctgtgcccg
       61 aacgaggcgc tgcgccagaa gctcaacggc atcgaactgc tgatcgagaa ctggcacacg
      121 ctgatgccgg cgcgaccgcc ggaacgctcg gtggtgcgca agggcgcgga aaacgatgaa
      181 gcctatgtgc ggcgcgtgct cggtccgctg gcggccagcc gcaacctgat cgtcctcaac
      241 gacgaggcac accatgccta ccgccggccg gcggagctca agatcagccg caaggatgtc
      301 gagtccctcg gcatcgatct cgaagaagcc acctgctgga tcgccgggct cgaccgcatc
      361 caccggcagc ggcgcatcgt gcgctgcttc gatctgtcgg caaccccgtt cgcgccgacc
      421 ggcaagacca gcaccgaagc ggcgctgttc gactgggtgg tttcggactt cggcctcaac
      481 gacgccatcg aggccggact ggtcaagaca ccgcgcgtcg tgatccgcga cgacgccctg
      541 ccggacgccc gcacctaccg cagccggctg tatcacctgt accgggagac cgaggtcggc
      601 gaagacatca accgccgcgg cgcacaagcc accgaagccc tgccgcaact ggtgcgcatc
      661 gcctacacgc tgctcggcgc cgactggcgg gcgacccgcc agagctggca ggccgccggc
      721 cacgacattc cgccggtgat gctgaccgtc tgcaaccgca ccgaaaccgc ggcccgcatc
      781 gagcactact tcggcagcgg cgatgtgcag tggccggaac tgcaggcccc cgaacgcacg
      841 ctgcgcgtcg attcgaaggt gctcgccaag gccgaagccg gcgaggctgc cggcagcgac
      901 aaggcttacg aggaacgttt gcaggccatc gtcgcagcga ccgacattcc ggccacgcgc
      961 aaggccgaac tgcgggcact gaagaaggag gaactgctgc gcgccatcgt cgataacgtc
     1021 ggcaagcgcg gcacgggcgg acaggacctg cagaacgtga tttcggtggc gatgctctcg
     1081 gaaggctggg atgcgaagaa cgtcacgcac atcctcggcc tgcgcgcctt caccagccag
     1141 ttgctgtgcg agcaggtggt cggccgcggc ctgcggcgcg tggcctacga gaccgaaacc
     1201 gtgcgctgcc cggatggcgt cgagcgcgag ctgttccggc ccgaatacgt caacgtgttc
     1261 ggcgtgccgc tgacggtgtt tcaggacagc gaggaaggcg gtgatccgcc gccgccgccg
     1321 cgcccgagca cggccgtgca gtcgctcgcc gagcgcagcg acttcgagat cgtctggccg
     1381 aacgtgctgc gcatcgatgc cgtcgtgcgc ccggaactga cgatcgactg ggcgcaggtg
     1441 ccggcgctga agatcgatcc ggcgcgcatc cagctcagcg ccgagctggc gccggcgctc
     1501 ggcgatgccg ccgatctgtc gcgcgccgtc agcatcgatc ttgaaaagct gccggccgat
     1561 ttccgcctgc aacgcctgct gttcgtcgcc gcgcgcaagg cattcgagca gttgcgtccg
     1621 ggctacgaag gcagccgcga gtggctggtg cagcaactga tccggctggt cgagcgcttc
     1681 ttcgcgtcgg accggctgga gattccgtcg cgcttccatc aggacccgct gcgccggcgc
     1741 atcctgatcg cgctgtcgat cgacgagatc gttcagcacg tcatcgggca gatcgagcag
     1801 cgcaacgtcg agcggctgga gccggtgttc gatccgcaat ggccgatcgg ctcgacccgg
     1861 cagatgcgca cctggtacac gacccggccg tgtctcgaaa ccgcgaagtc gcagatcagc
     1921 cacgcggtcg ccgacagcac ctgggagcag tacacggtcg ggcggctgga agcctgcgcc
     1981 ggagtcgccg ccttcgcgcg caatgaccat ctcggctttc aggtgcacta cctctggcac
     2041 ggcagccgcc ggcgctacgt gccggacttc ctgatccgct tcgcaaacgg caagcagatg
     2101 gtgctggaga tcaagggcga ggactcggag caggaccgct gcaagcgccg tgcgctcgat
     2161 gcctgggtgc aggcggtcaa cagccgcggc ggtttcggcg tctggtgccg ggatgtggtc
     2221 tgcggcgagc cggcgcgcat cgacgacgtg ctcgaccatc acgccggtac gggcggggcg
     2281 tcgatgccgg tcggtgaggc gcagttcagc ctcgggaccg gatgaggcag ccggctgcca
     2341 tattccacat cctgaaaacc attgcatccg cactggaaag ccggcgtatt tctgcggcat
     2401 gc
//
LOCUS       DAOQIE010000437         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_32152_length_2397_cov_8.712639, whole genome shotgun sequence.
ACCESSION   DAOQIE010000437 DAOQIE010000000
VERSION     DAOQIE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32152_length_2397_cov_8.712639"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK144_17945"
     CDS             complement(<1..570)
                     /locus_tag="PK144_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNF67658.1"
                     /translation="MNLTELKRKPASEIIEIAQELGLEGMARSRKQDVIFAILKAHAK
                     SGEDIWGDGVLEILSDGFGFLRGGDSSYLAGPDDIYVSPSQIRRFNLRTGDTVSGKIR
                     PPKEGERYFALLKVNEINYEPPENSKSKVLFENLTPLHAHRRLKLERGNGSTEDITAR
                     IIDLIAPIGKGQRGLIVSPPKAGKTVLMQN"
     gene            complement(821..1147)
                     /gene="trxA"
                     /locus_tag="PK144_17950"
     CDS             complement(821..1147)
                     /gene="trxA"
                     /locus_tag="PK144_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNF67659.1"
                     /translation="MSDQIVSVSDSSFESDVLKSELPVLVDYWAEWCGPCRMIAPILD
                     KVAEDYKGRLTVAKLNIDENQVTPTRYGVRGIPTLMIFRGGEIIGTKVGALSASQLSK
                     FIEDTI"
     gene            1279..>2397
                     /locus_tag="PK144_17955"
     CDS             1279..>2397
                     /locus_tag="PK144_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008314297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF67660.1"
                     /translation="MEKSHLTSTPFSALPLTDCLLEALEDAGFTYCTPIQDATLPLAL
                     AGHDIAGQAQTGTGKTAAFLLSTLHYLMTTPVEDGLSGPWAIMLAPTRELALQIHKDA
                     ESLGRYTGLKFAAVYGGTGYDSQRRQLEDGVDVVIGTPGRIIDFYKQGVLKLKHIEVV
                     VLDEADRMFDLGFISDIRYLLRRMPSADQRINLLFSATLSTRVMELAYEHMNNPQQIK
                     IESEQVTADKVRQTLFHVSKEDKIPLLVGLLHREKPNRAIVFVNTKRAAEQVEGWLAG
                     NSWQCAVLSGDIPQNKRERLLAQFQAGELPVLVATDVAARGLHIPDVSHVFNFDLPQD
                     AEDYVHRIGRTARIGASGDAISFACEEYVYSLPDIEDYI"
BASE COUNT          408 a          819 c          768 g          402 t
ORIGIN      
        1 gttctgcatc agcacggtct tgccggcctt cggcggcgag acgatcaggc cgcgctggcc
       61 tttgccgatc ggcgcgatca ggtcgatgat ccgcgccgtg atgtcctcgg tcgaaccgtt
      121 gccgcgttcg agcttcaggc gccggtgcgc gtgcagcggc gtcaggttct cgaacagcac
      181 cttgctcttg gagttctcgg gcggttcgta gttgatctcg ttgaccttca gcagcgcgaa
      241 gtagcgctcg ccttccttgg gcgggcggat cttgccgctg acggtatcgc cggtgcgcag
      301 gttgaagcgc cggatctgcg acggcgacac gtagatgtcg tcgggtccgg ccagatacga
      361 actgtctccg ccgcgcagga agccgaaacc gtcggagagg atttcgagca cgccgtcgcc
      421 ccagatgtcc tccccgctct tcgcgtgcgc cttcaggatc gcgaagatca cgtcctgctt
      481 gcgcgaacgc gccatgcctt cgaggcccag ctcctgcgcg atctcgatga tctcggacgc
      541 gggttttctc ttgagttcag tcagattcat gtaggcgtgc ttccggcgcg ggggctgcgg
      601 aatggcaccg gcccggggtg cggagaaggg cctggatgcg cggtgcgggg gctggcgggg
      661 tcgacggggg acgtcgaggg tgcgggggaa gcaccggaga cgggcgcccg cagaaaaaat
      721 gatggcggaa gctaacacgc gacacggccg gcgtccagaa gtcggacgcc ggccggattc
      781 gggcgaaacg gccgggcgcg gcgcggcccg gcggcgggtt tcagatggtg tcctcgatga
      841 acttgctcag ctgcgaggcc gacagcgcgc ccaccttggt gccgatgatc tcgccgccgc
      901 ggaagatcat cagcgtcggg atgccgcgca cgccgtaccg ggtcggcgtc acctgattct
      961 cgtcgatgtt gagcttggcg acggtcagcc ggcccttgta gtcctcggca accttgtcga
     1021 ggatcggggc gatcatgcgg cacggaccgc accattcggc ccagtagtcg accagcaccg
     1081 gcagttccga cttcaggaca tcgctttcga agctgctgtc ggacacagaa acgatttgat
     1141 cactcacggc attcgcctcc agaatggcgc cgctcacacg gtcgccttag caaaccaaag
     1201 actcatttca gccgaaacgc cggaacgctg gcaagtgtcg gcaccggccg ccctcccgcg
     1261 gtatcccctg cgctccccat ggaaaaaagc catctgacct ccacgccgtt ctcggcgttg
     1321 cccctgaccg actgcctgct ggaagccctc gaagacgccg gattcacgta ctgcaccccg
     1381 atccaggacg ccacgctgcc gctcgcgctc gccggtcacg acattgccgg ccaggcccag
     1441 accggcaccg gcaagacggc cgccttcctg ctgtccacgc tgcactacct gatgacgacg
     1501 ccggtcgagg acggactctc cggcccctgg gcgatcatgc tcgcccccac ccgcgaactc
     1561 gcactgcaga tccacaagga cgccgaatcc ctcggccgct acaccggtct gaagttcgcc
     1621 gccgtctacg gcggcaccgg ctatgactcg cagcgccgcc agctggagga cggcgtcgat
     1681 gtcgtcatcg gcacgccggg ccggatcatc gacttctaca agcagggcgt gctgaagctc
     1741 aagcacatcg aagtcgtcgt gctcgacgag gccgaccgca tgttcgacct cggcttcatc
     1801 tccgacatcc gctacctgct gcgccggatg ccttcggccg accagcgcat caacctgctg
     1861 ttctcggcga cgctgtcgac ccgggtcatg gagctcgcct acgagcacat gaacaacccg
     1921 cagcagatca agatcgagtc cgaacaggtc accgccgaca aggtgcgcca gacgctgttc
     1981 cacgtctcga aggaagacaa gatcccgctg ctggtcggcc tgctgcaccg cgagaagccg
     2041 aaccgcgcca tcgtgttcgt caacaccaag cgcgctgccg agcaggtcga gggctggctg
     2101 gccggcaaca gctggcagtg cgcggtgctc tccggcgaca tcccgcagaa caagcgcgag
     2161 cgcctgctcg cgcagttcca ggccggcgag ctgccggtgc tggtggcgac cgatgtcgcc
     2221 gcacgcggcc tgcacatccc ggacgtgagc cacgtcttca acttcgacct gccgcaggac
     2281 gccgaggact acgtgcaccg catcggccgc acggcgcgca tcggcgcctc gggcgacgcg
     2341 atcagcttcg cctgcgagga gtacgtctac tcgctgccgg acatcgagga ctacatc
//
LOCUS       DAOQIE010000438         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_32368_length_2388_cov_7.213459, whole genome shotgun sequence.
ACCESSION   DAOQIE010000438 DAOQIE010000000
VERSION     DAOQIE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32368_length_2388_cov_7.213459"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="sufB"
                     /locus_tag="PK144_17960"
     CDS             complement(<1..856)
                     /gene="sufB"
                     /locus_tag="PK144_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNF67661.1"
                     /translation="MSANLEELADRGYAYGFTTDVEQETVPPGLSEDVIRLISAKKQE
                     PEWLLEWRLEAYRSWLKMPVPEWAHVHYPPIDYQAISYYSAPKKSIGDGPASLDDIDP
                     EIKKTYDRLGIPLVEQEFLAGVAVDAVFDSVSVATSYRGKLAEQGIIFCSFSEAVREH
                     PELVRQYLGTVVPVRDNYYAALNSAVFSDGSFVYIPKGVRCPMELSTYFRINAKNTGQ
                     FERTLIVADAGAYVSYLEGCTAPMRDENQLHAAVVELVAMDDATIKYSTVQNWYPGDK
                     EGRGGIYNF"
     gene            complement(853..1323)
                     /locus_tag="PK144_17965"
     CDS             complement(853..1323)
                     /locus_tag="PK144_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HNF67662.1"
                     /translation="MIRISKETDYGIVLLSCLAQAADASLSASALAERGRLPLPTVSK
                     VLKLLARGGLLVSQRGARGGYALARPPREITVAEIIAVIDGPIALTDCSPGGHGDCGY
                     QGHCQVSGHWGPISRAISTALEGVTLADLLRPQAPAVVTVPFVIHHAVPGSPSA"
     gene            1794..2321
                     /locus_tag="PK144_17970"
     CDS             1794..2321
                     /locus_tag="PK144_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF67663.1"
                     /translation="MRPALPRRLALLAALATALVACTPEAPLRPRPPFEPADRILVEK
                     AARRLTLYSGERTLRSYRVSLGFAPVGPKRQEGDGKTPEGHYRIDWRNPDSRFHRSLH
                     VSYPDAGDVARAAARGVSPGGLIMIHGLPNGFGALGDWHRTRDWTDGCIAVTNDEMDE
                     IWALVADGTPIEIRP"
BASE COUNT          390 a          811 c          822 g          365 t
ORIGIN      
        1 cgaagttgta gatgccgccg cggccttcct tgtcgcccgg ataccagttc tgcacggtcg
       61 agtacttgat cgtcgcgtcg tccatcgcca ccagctcgac gaccgccgcg tgcagctggt
      121 tctcgtcgcg catcggcgcc gtgcagcctt ccaggtagct cacgtaggcg ccggcatcgg
      181 cgacgatcag cgtgcgctcg aactggccgg tgttcttggc gttgatgcgg aagtaggtcg
      241 acagctccat cgggcagcgc acgcccttcg ggatgtagac gaaggagccg tccgagaaca
      301 ccgccgagtt cagcgccgcg tagtagttgt cgcgcaccgg cacgaccgtg ccgaggtact
      361 ggcgcacgag ttccggatgc tcgcgcacgg cctccgagaa cgagcagaag atgatgccct
      421 gctcggcgag cttgccgcgg tagctggtcg cgaccgacac cgaatcgaac acggcatcga
      481 cggcgacgcc ggccaggaat tcctgttcga ccagcgggat gccgagccgg tcgtaggtct
      541 tcttgatctc ggggtcgatg tcatcgagcg aggccgggcc gtcgccgatc gacttcttcg
      601 gtgccgagta gtagctgatc gcctgatagt cgatcggcgg ataatgcacg tgcgcccact
      661 ccggcaccgg catcttcagc cagctgcggt aggcctccag ccgccattcc agcagccact
      721 cgggctcctg cttcttggcc gagatcagcc ggatcacgtc ctcgctgagg ccgggcggca
      781 cggtttcctg ttcgacgtcg gtggtgaaac cgtacgcgta gccgcgatcg gccagttctt
      841 cgagatttgc actcatgcgg acggactccc cggcacggcg tgatggatga cgaagggcac
      901 ggtcacgacc gcgggcgcct gcgggcgcag caggtcggcg agcgtgacac cttcgagggc
      961 ggtgctgatc gcgcggctga tcggccccca gtggccgctg acctgacagt ggccctgata
     1021 gccgcagtca ccgtggccgc ccgggctgca gtcggtcagc gcgatcgggc cgtcgatgac
     1081 ggcgatgatc tcggcaaccg tgatctcgcg cggcgggcgc gccagagcgt agccgccacg
     1141 tgcgccgcgc tgcgaaacca gcagcccgcc gcgtgccagc agtttcagca ccttgctgac
     1201 ggtaggcagc ggcagccggc cgcgttcggc gagcgcgctg gcactgaggg aagcgtccgc
     1261 ggcctgcgcg agacaggaca gcagcacgat gccgtagtcg gtctccttgc tgatgcggat
     1321 catggacgcc ccgtaatcag caccggaaag gtgctatatg ggcgcagcat acgactgcaa
     1381 gcaggactag ggaagtcctc atttcgggga agacgcggca gacgcatgga gcagcccgga
     1441 aaacgggccg ctgcgtccgg catcgatgcc cgcgggcgcg ggcggaggaa ggcaggaagt
     1501 gcgcgggctc aggcccgagg cagcgcggtg tcgttcagtg gcgcagccag ctccagcact
     1561 ggcgcagcag tccccacaga ctctggcggg catgggcagg accaccgtgc cggcgcagcg
     1621 aggcaagctg gttgcgggtc gcgtaactca tctctcggct ccggcaggct gaacagggag
     1681 ccgatgataa tcattatcga atacgaatca cagccggatc tttgccgctg tccgcagcgc
     1741 cgcggactgc gcacaatgcc tgcgacaccc gctcccccgg aggaaaccgg ccgatgcgcc
     1801 ccgccctgcc gcgccgcctg gccctgctcg ccgccttggc cacggccctc gtcgcctgca
     1861 cgccggaagc cccgctgcgg ccgcgtccgc cgttcgagcc cgccgaccgc atcctggtcg
     1921 agaaggccgc gcggcggctg acgctgtatt cgggcgaacg gacgctgcgc agctaccgcg
     1981 tctcgctcgg cttcgcgccg gtcggcccga agcgccagga aggcgacggc aagacccccg
     2041 aaggccatta ccggatcgac tggcgcaacc ccgacagccg cttccaccgc tcgctgcacg
     2101 tgtcgtatcc ggacgccggc gatgtcgcgc gagccgcggc gcgcggcgtg tcgccgggcg
     2161 ggctgatcat gatccacggt ctgccgaacg gcttcggcgc gctcggtgac tggcaccgga
     2221 cacgcgactg gaccgacggc tgcatcgccg tcaccaatga cgagatggac gaaatctggg
     2281 cactggtcgc cgacggcacg ccgatcgaga tccggccgtg aagcgcggcc gcttcagccg
     2341 cgcagcgcct gcgtcagcac gcgatcgagg gccgaggcga actgctgg
//
LOCUS       DAOQIE010000439         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_32935_length_2363_cov_8.108319, whole genome shotgun sequence.
ACCESSION   DAOQIE010000439 DAOQIE010000000
VERSION     DAOQIE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32935_length_2363_cov_8.108319"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..415
                     /locus_tag="PK144_17975"
     CDS             128..415
                     /locus_tag="PK144_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67664.1"
                     /translation="MNSEQAILAAIERIPLPEPVERLIAYPEVDSMDRPAIRVWIILK
                     DDHVAERETSAMLDALTQAIRDRTWQIDERYWPYVRVRSVSEQALIESEHG"
     gene            412..924
                     /locus_tag="PK144_17980"
     CDS             412..924
                     /locus_tag="PK144_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67665.1"
                     /translation="MSLPADLLKQAEHLARKEPRRPSQASLRRAVSAAYYALFHRLIE
                     DAAARLVPGTHRRALRQRIARACEHGEMRRVCERFAQPAGRLPPGLQTLLPGGVPPAL
                     QQVAATFVELQEARHEADYNLASSLTRTDAQRLIELARRACSTWVTVRECEESRVFLL
                     AFLFFKQLQR"
     gene            943..1815
                     /gene="hslO"
                     /locus_tag="PK144_17985"
     CDS             943..1815
                     /gene="hslO"
                     /locus_tag="PK144_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNF67666.1"
                     /translation="MTDALHRFLFESHPVRGEFATLDGLWQTVLERRDYPAPVRRVLG
                     EAMAAAALLSATIKYTGVLTLQLQAPDGRLRLLVVQVHSDRTLRGLARWDETAGLPDE
                     LPLAGLCGHGTLVISIETEGAREPYQGIVPLDGESIAAALEHWFAQSEQLPTRLVLAA
                     DAQRAAGLLLQRLPGVDADADHWQRVTQLGATLTAAELLSEAPETLLHRLFHEEDVRT
                     FAPEALAFRCSCSRARSAAALRTLGAEELRALLAERGGTVGVDCEFCGQAYTFDAVDI
                     EALLAAADAPPAAQ"
     gene            1846..2358
                     /locus_tag="PK144_17990"
     CDS             1846..2358
                     /locus_tag="PK144_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67667.1"
                     /translation="MSALVQLFLDIMLMRRGPQDVPASTALCAVCTVAYALLGFLSVL
                     LNSPPVLALAQTVLDLVLLTGFTQVLLGAHRHRARFLQTYTALTGTGALFAIAAAPVL
                     LAFPNDPGQGTPSLVAFLYFGLVIWSIAVMGHVYAQALSTARGRGLAWSIAYFVLSFV
                     ALAFLYAQFD"
BASE COUNT          336 a          812 c          808 g          407 t
ORIGIN      
        1 accccagccg cagccgcagc gtccgaaacc gcgtggcgac gacagcttct tctgagcact
       61 ccagaagtat ttcatgtgag gatttgcgct ggctgcgtgt gccggtgcct gacgggttga
      121 ggcggtcatg aacagcgaac aggccatcct cgctgcgatc gaacggattc cgttgccgga
      181 accggtcgag cggctcatcg cttacccgga ggtcgattcg atggatcgac cggctatccg
      241 cgtctggatc atcctgaagg atgatcacgt tgccgagcgc gagacgagcg ccatgctgga
      301 tgcgctgacg caggcgatcc gtgaccggac gtggcagatc gacgagcgct actggcctta
      361 cgtgcgcgtg cgcagcgtca gcgaacaggc gctcatcgaa agtgagcatg gatgagtctg
      421 cccgccgatc tgctgaagca ggccgaacat ctggcgcgca aggagccacg ccggccgagc
      481 caggccagtc tgcgccgggc ggtttcggcc gcgtattacg cgctgttcca tcgtttgatc
      541 gaggatgccg ccgcccggct cgttcccggg acgcaccgcc gagccttgcg ccagcgcatc
      601 gcacgggcgt gtgagcacgg agagatgcgc cgggtctgcg agcgattcgc ccagcccgcg
      661 ggcaggctgc cgccgggttt gcaaacgctg cttccgggcg gtgttccacc ggcgttgcag
      721 caagtcgcgg cgaccttcgt cgaactgcag gaggcgcggc acgaagcgga ctacaacctg
      781 gcgtcgagtc tgacccggac cgatgcgcaa cgcctgatcg aactcgcacg ccgcgcctgc
      841 agtacatggg tgacggtgcg tgagtgcgag gagagtcgcg tgttcctgct tgcctttctg
      901 tttttcaagc aactccagcg ctgagcacga atcgactgtc ccatgaccga cgccctgcac
      961 cgcttcctgt tcgaatccca tcccgtgcgc ggcgaattcg cgacgctcga cggcctctgg
     1021 cagaccgtgc tggagcggcg cgactatccg gcgccggtgc ggcgcgtgct cggcgaagcc
     1081 atggccgccg cggcgctgct gtcggcgacg atcaagtaca ccggcgtgct gacgctgcag
     1141 ctgcaggcgc ccgacggacg gctgcgcctc ctggtcgtgc aggtgcattc ggaccgcacg
     1201 ctgcgcggcc tcgcgcgctg ggacgaaacc gccggactgc ccgatgaact gccgctcgcc
     1261 gggctctgcg gccatggcac gctggtgatc agcatcgaga ccgagggcgc gcgcgagccg
     1321 tatcagggca tcgtgccgct cgatggcgag tcgatcgccg cggcgctcga acactggttc
     1381 gcgcaatccg aacagctgcc gacccggctc gtgctcgccg ccgatgcgca gcgcgcggcc
     1441 ggcctgctgc tgcagcgcct gcccggcgtc gatgccgatg ccgatcactg gcagcgggtc
     1501 acgcagctcg gcgcgacgct gacggcggcc gaactgctgt cggaagcacc ggaaaccctg
     1561 ctgcaccggc tgtttcacga ggaggacgtg cggaccttcg cgcccgaggc gctcgcgttc
     1621 cgctgcagct gctcgcgcgc gcgttcggcg gcggcgctgc gcacgctcgg ggccgaagaa
     1681 ctgcgcgcgc tgcttgccga gcgcggcggc acggtcggcg tcgactgcga gttctgcggc
     1741 caggcctaca ccttcgatgc cgtcgacatc gaggcactgc tcgctgccgc cgatgcaccg
     1801 ccggcggcgc aatagcatgg acttcctccc gatgccggtc cggacatgag tgccctggtg
     1861 cagctgttcc tcgacatcat gctgatgcgc cgcggtccgc aggacgtgcc ggcctcgacg
     1921 gcgctgtgtg cggtctgcac ggtcgcctac gcgctgctcg gcttcctgtc ggtgctgctc
     1981 aacagcccgc cggtgctggc gctggcgcag accgtgctcg atctggtgct gctgaccggc
     2041 ttcacgcagg tgctgctcgg cgcgcaccgc caccgtgcgc gcttcctgca gacctacacg
     2101 gcgctcaccg gcaccggcgc gctgttcgcg atcgccgccg cgccggtgct gctcgcgttc
     2161 ccgaacgatc ccggccaggg cacgccgtcg ctggtggcct tcctgtactt cgggctggtg
     2221 atctggagca tcgccgtcat gggccacgtc tacgcgcagg cgctgtcgac cgcgcgcggt
     2281 cgcggactgg cgtggtcgat cgcctacttc gtgctgagct tcgtggcgct tgcgttcctg
     2341 tatgcccagt tcgattgata ccg
//
LOCUS       DAOQIE010000440         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_33096_length_2357_cov_6.288879, whole genome shotgun sequence.
ACCESSION   DAOQIE010000440 DAOQIE010000000
VERSION     DAOQIE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33096_length_2357_cov_6.288879"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..2168
                     /locus_tag="PK144_17995"
     CDS             276..2168
                     /locus_tag="PK144_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNF67668.1"
                     /translation="MPFLKNLSLRSQFALLLAINALAMVLALTVTLRNLHAELEHERR
                     DQVSNLVDLAWATVARYQAAEAAGQLDRATAQARAIEALLGMRSGEIYFWVHRSDGVY
                     VMHGGKPERAGTAGLLELRDAQGRRLFDSFEDALARGNGAGFVEYSFPRAGTDVPEPK
                     VSRVKRFVPWDWVIGSGLYISDVEDAYASQRNQLLVGLGAALLLLAVMLWGQSRRIVR
                     QVHAVLGLARRLAARDLSNPLPVEARDEFGQMAEALNAAVTAMQTAFTEVEEAAARDR
                     QQMEQLRAAEAAQETQSREIAAQADRIRAAAEREHAAAEELRAKVDRIVVAVEAAARG
                     DLTAEAGISGDDAIGRVGRALTRLLTDLREGIGGIGRNAGALGRSAHDFTDVSHQLNE
                     LAAATASGADRLREAAHAVDLNLQTVSTSTDEMNSAVNEISRNVHRAVEIAAGAGGAA
                     DHASKLVAQLAASSQEIGSVSRTIAGIAEQTNLLALNATIEAARAGEAGKGFAVVASE
                     VKDLARGTAQATGDIERRIEAIQTDTQHVVNAIDAIRRTIEEVNDISTMIATAVEQQS
                     VTTAEIGRSVSSAAQGGSEINAAAQRMADTVGSLHAGAASTAQAATELSKLAQDQTTL
                     LGRFRC"
BASE COUNT          372 a          874 c          799 g          312 t
ORIGIN      
        1 cgtcacggtt ttcagccggc ccgggcgcac acagatgcct gtctgcgcgg gcgctttcgg
       61 ccgcgtcatc cggattttgc cgcattgcgc aatggcagcg gctcgctgcc gcccggcaaa
      121 accttaccgc ccgcccggta aatctttgta ttggcctata gccgcccgcc cgccctgccg
      181 atattcagtc gcgatacccg atacgtccgc ccgtggcgcc ggccgtcccc cccgaccgcc
      241 ggccctgccg tatccccgca accgggagcg tcgcgatgcc tttcctgaag aacctgtccc
      301 tgcgcagcca gttcgcgctg ctgctcgcga tcaacgcgct ggccatggtg ctggcgctga
      361 ccgtgacgct gcgcaacctg cacgccgagc tggagcacga acgccgtgac caggtttcga
      421 atctggtcga tctggcctgg gcaacggtcg cgcgctatca ggccgccgag gccgccggac
      481 agctcgaccg ggccacggcg caggcgcgcg ccatcgaggc gctgctcggc atgcgcagcg
      541 gcgagatcta cttctgggtg catcgctcgg acggcgtcta tgtgatgcac ggcggcaagc
      601 cggagcgtgc cggcaccgcc gggctgctgg agctgcggga tgcgcagggc aggcgcctgt
      661 tcgacagctt cgaggacgcg ctcgcgcgcg gcaacggcgc cggcttcgtc gagtacagct
      721 ttccgcgggc cggaaccgat gtgcccgagc cgaaggtctc gcgggtcaag cgcttcgtgc
      781 cctgggactg ggtgatcggc agcggcctgt acatctccga tgtcgaggac gcctacgcca
      841 gccagcgcaa ccagttgctc gtcggcctcg gcgcggcgct gctgctgctc gccgtgatgc
      901 tgtgggggca gagccggcgc atcgtgcgcc aggtgcatgc ggtgctcggc ctcgcgcgcc
      961 ggctggcggc gcgcgatctc agcaatccgc tgccggtcga ggcgcgcgac gagttcgggc
     1021 agatggccga ggcgctgaac gccgcggtca ccgcgatgca gaccgccttc accgaggtcg
     1081 aggaagccgc ggcgcgcgac cgccagcaga tggagcagct gcgcgccgcc gaagccgccc
     1141 aggaaaccca gtcgcgcgag atcgccgcgc aggccgatcg catccgtgcc gccgccgaac
     1201 gcgagcacgc cgcggccgag gaactgcgcg ccaaggtcga tcgcatcgtc gttgccgtcg
     1261 aggccgccgc acgcggcgac ctgaccgccg aagccgggat cagcggcgac gatgcgatcg
     1321 gccgcgtcgg ccgggccctc acccggctgc tcacggacct gcgcgagggc atcggcggca
     1381 tcggtcgcaa cgccggcgca ctcgggcgtt cggcgcacga cttcaccgac gtgagccacc
     1441 agctcaacga gctggccgcc gccaccgcca gcggcgccga ccggctgcgc gaggccgcgc
     1501 acgccgtcga cctgaacctg cagaccgtct cgacctcgac cgacgagatg aactcggccg
     1561 tcaatgaaat ctcgcgcaac gtccaccgcg cggtcgagat tgctgccggc gccggcgggg
     1621 ctgccgatca cgcctcgaag ctggtcgcgc agctcgccgc cagcagccag gagatcggct
     1681 cggtcagccg cacgatcgcc ggcatcgccg agcagaccaa cctgctggcg ctgaatgcga
     1741 cgatcgaggc ggcgcgcgcc ggcgaggccg gcaagggctt cgccgtcgtg gccagcgagg
     1801 tcaaggatct cgcccgcggc acggcgcagg ccaccggcga catcgagcgg cgcatcgagg
     1861 cgatccagac cgacacgcag cacgtcgtca acgccatcga cgcgatccgc cgcacgatcg
     1921 aggaggtcaa cgacatctcg acgatgatcg cgaccgccgt cgagcagcag agcgtgacca
     1981 ccgccgagat cggccgcagc gtcagcagcg ccgcgcaggg cggcagcgag atcaacgccg
     2041 ccgcgcagcg catggccgat acggtcggca gcctgcacgc cggtgcggcc agcaccgcac
     2101 aggccgccac cgagctttca aaactcgcgc aggaccagac aacgctgctc ggccgcttcc
     2161 gctgctgaag ccccgggctg gcgtgccgat aagccaacgt acctttgacc agtgtttgac
     2221 ggacgacggc ttgagacggc cgctccccgc ccgaacaatg ccgcgctccc ccgccaccgg
     2281 ggccgcaccg atgccaaccg ggatgacgcc atgacccagc tgcccctgcg ctcgcagttc
     2341 aaattcgcca ccgtcgt
//
LOCUS       DAOQIE010000441         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_33431_length_2343_cov_7.173514, whole genome shotgun sequence.
ACCESSION   DAOQIE010000441 DAOQIE010000000
VERSION     DAOQIE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33431_length_2343_cov_7.173514"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PK144_18000"
     CDS             <1..1308
                     /locus_tag="PK144_18000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNF67669.1"
                     /translation="TSPVFRDTVFAMQRDEVVTMAVDAVRQIRALTDARPETHWTLEY
                     SPETFSATELEFAREICDAVTDAWGATPERKVILNLPATVEMATPNVYADQIEWMHRN
                     VARRNSVIISVHPHNDRGCAVAAAELAMMAGAERVEGCLFGHGERTGNVDLVTLALNL
                     YSQGVDPQLDFSDIDRIARTVEECTQLPVHPRHPYTGDLVFTAFSGSHQDAIKKGMAV
                     RDDATHWQVPYLPVDPSDLGRSYDSIIRVNSQSGKGGIAYLLESGYGLVMPRRLQIEF
                     SGVVQQHTDATGAEVSADELWQIFNRSYVQANAPLRYVEHHLFEHGDAQGVKITVAMG
                     ERRFTVSGEGNGPIDAIIHALRVPVRLLAYEERSLGQGADARAVAFVEMAGGDQPGST
                     HGVGIDDNIVTASIFAVLSGINRIAARDEAIVADLDRRTLQAA"
     gene            complement(1357..>2343)
                     /locus_tag="PK144_18005"
     CDS             complement(1357..>2343)
                     /locus_tag="PK144_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF67670.1"
                     /translation="VSPAFLDMIGIGSAEVAGCDEARFAALMTSRSKQEAAFPGFDTM
                     RADALSRREDGGDRSRRFLMALSSPAGRVLEIGIRLADGEPVSQVLYFRDVSHEVEVD
                     RMKSEFLSTAAHELRTPMASIYGYSELLLAQEFPESERKEVCGIIFRQSELMVSIINE
                     LLDLVRIEERRGKDFSFARVDAAELLREAAGNVKAPEGRERPLADFPDQPHWLRADRK
                     KLMQAIGNVVSNAYKYSPAGGCVRMQIITDDEHHPGMCGLRIGDEGIGMTLEQQARVC
                     ERFYRADASGKIPGTGLGMSIVKEIIELHGGRVDIRSESRAGTEVTLWVPLA"
BASE COUNT          415 a          831 c          726 g          371 t
ORIGIN      
        1 acctcgccgg tcttccgcga taccgtcttt gcgatgcagc gggacgaggt ggtgacgatg
       61 gcggtcgacg ccgtacgcca gatccgtgcg ctcaccgatg cacggccgga aacgcactgg
      121 acgctcgaat acagcccgga aaccttctcg gcgaccgaac tcgagttcgc ccgtgagatc
      181 tgcgacgcgg tcacggacgc ctggggcgcg acgccagaac gcaaggtgat cctcaacctg
      241 ccggcgacgg tggagatggc aacgcccaac gtatacgccg accagatcga gtggatgcac
      301 cgcaacgtcg cacgccgcaa cagcgtgatc atcagcgtgc atccgcacaa cgaccgcggc
      361 tgcgccgtcg ccgctgccga actggcgatg atggccggtg ccgagcgcgt cgagggctgc
      421 ctcttcggcc acggcgaacg cacaggcaac gtcgaccttg tcacgctcgc gctcaatctc
      481 tactcgcagg gggtcgaccc gcagctcgat ttttccgaca tcgaccgcat cgcgcgcacc
      541 gtcgaggaat gcacgcagtt gccggtgcac ccgcgccacc cctacaccgg cgaccttgtc
      601 ttcaccgcct tctccggctc gcaccaggat gcgatcaaga agggcatggc ggtgcgcgac
      661 gacgccacgc actggcaggt cccctacctg ccggttgacc cgagcgacct cggccgcagc
      721 tacgactcga tcattcgcgt gaacagccag tccggcaagg gcggcatcgc ctacctgctc
      781 gaatcgggct acggcctcgt catgccgcgc cgcctgcaga tcgagttcag cggtgtcgtg
      841 cagcagcata ccgacgccac cggcgccgaa gtcagcgccg atgaactctg gcagatcttc
      901 aaccgcagct acgtgcaggc caacgcgccg ctgcgctacg tcgagcacca tctcttcgag
      961 catggcgatg cgcagggcgt gaaaatcacc gtcgcgatgg gcgaacgccg cttcacggtc
     1021 agcggcgaag gcaacggacc gatcgatgcc atcatccacg cgttgcgcgt gccggtgcgc
     1081 ctgctcgcct acgaggagcg ctccctcggg cagggcgccg atgcccgcgc cgtggccttc
     1141 gttgaaatgg ccggcggcga ccagcccggc agcacgcacg gcgtcggcat cgacgacaac
     1201 atcgtcacgg cctcgatctt cgccgtgctc tccggcatca accgcattgc tgcacgcgat
     1261 gaggccatcg tggcggacct cgaccgccgc accctgcagg ccgcctgacg cctggcagcc
     1321 ccccccgggc acggcacctg ccgcgccccg gggcgttcac gccagcggca cccagagggt
     1381 cacttcggtc ccggcgcggg attcgctgcg gatatcgacg cgaccgccgt gcagttcgat
     1441 gatctccttg acgatgctca tcccgaggcc ggttccggga atctttcccg aagcgtcggc
     1501 ccgatagaag cgctcacaga cacgagcctg ctgctccagg gtcatgccga tgccctcgtc
     1561 gccgatccgc aggccacaca tgccgggatg atgctcgtcg tcggtgatga tctgcatgcg
     1621 gacacagcca ccggccgggg aatacttgta ggcattcgaa acgacattcc cgatggcctg
     1681 catcagtttc ttgcgatcgg cccgcagcca gtgcggctgg tcgggaaaat cggccaacgg
     1741 ccgctcgcgc ccttccggcg ccttgacgtt gcccgcagcc tcccgcagca gttcggcggc
     1801 gtcgacccgc gcaaagctga aatccttgcc gcggcgctcc tcgatgcgta ccagatcgag
     1861 aagctcgttg atgatcgaca ccatcagttc ggactggcgg aagatgatcc cgcaaacctc
     1921 tttccgctcg gactcgggaa attcctgcgc gaggagaagt tccgagtagc catagatgct
     1981 ggccatcggt gtccgcagct cgtgcgcggc ggtcgacagg aattcgctct tcatgcgatc
     2041 gacctcgacc tcgtgtgaaa catcgcggaa atacagcacc tgcgataccg gctccccgtc
     2101 ggccagccgg atgccgattt cgagtacgcg cccggccggg ctggacagcg ccatcaggaa
     2161 gcggcgggaa cggtcgccgc cgtcctcacg acgcgagagc gcgtcggcgc gcatcgtgtc
     2221 gaagcccggg aacgccgcct cctgcttgct gcgagaggtc atcagcgccg cgaagcgcgc
     2281 ctcgtcgcaa ccggccacct ccgcactgcc gatgccgatc atgtcgagga atgccggact
     2341 gac
//
LOCUS       DAOQIE010000442         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_33451_length_2342_cov_9.536073, whole genome shotgun sequence.
ACCESSION   DAOQIE010000442 DAOQIE010000000
VERSION     DAOQIE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33451_length_2342_cov_9.536073"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PK144_18010"
     CDS             <1..1060
                     /locus_tag="PK144_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNF67671.1"
                     /translation="FALAAAVAVRQLLRPAPPAMALPDAAEAADLACRVAASPHTYAH
                     LARVGDKAILTSPDGDGFLMYAVQGRAWIAMGDPLGTPAERRELAWRLRERAETWAGW
                     TVFYQASPAHLDLYVELGLSLLKIGEEARVELSTFTLDGAARKNLRNTRNRLVRDGYG
                     FRVVPAAEVPALLGQLQAVSDAWLDGKQTREKGFSLGRFEAGYLGGGPVAVIEHAGRI
                     VAFANLWCGAQHAELSVDLMRFADDAPAGAMDALFVELLLWGKAEGYAWFNLGMAPLA
                     GLHARALAPLWNRFGALVFGRGERFYNFRGVRQYKEKFGPVWEPRYLAAPGGLRLPRV
                     LAAVTTLIAGGLSGVVQK"
     assembly_gap    1074..1173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1174..>2342
                     /locus_tag="PK144_18015"
     CDS             <1174..>2342
                     /locus_tag="PK144_18015"
                     /inference="COORDINATES: protein motif:HMM:NF017835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HNF67672.1"
                     /translation="ALFCTVACSTLTPATAETLNYGRFGDIAVLRPEGKPRSVTIVVS
                     GSGGPMQAEAEIGRLLNAQGSLVLSVDGERLLHNIRPKPDETCADAGWDFEAMSQYVQ
                     KELGLGDYRRPLLVGVGLGASLVFLGLAESTPNTFLGGMALGFDPDWRSPWRLCEGDQ
                     GEVGKPAADGRGWQLAPSDEYLLRWTVLQGAADPYVAGAKVAQFVEGVPNAELVTVPG
                     VGHGFAEPARWRDSFVDAWKRLNNDPYQDRELTELPLIELPVAQPSGDWLAVILSGDG
                     GWAAIDRELGETFQHEGIPVVGFDMLRYLWKARSPEETANAVASAIERYTQRWQRRRV
                     LLVGYSLGGDVLPFVVNRLPPQTRQKLALSAVLAPSKYVEFEFHPWDWISDDDDA"
BASE COUNT          300 a          776 c          825 g          341 t
ORIGIN      
        1 gttcgcgctg gctgcggcgg tcgcggtgcg ccagctgctg cgcccggcgc cgccggcgat
       61 ggcgctgccg gacgcggctg aggccgccga tctggcgtgt cgcgtcgcgg ccagcccgca
      121 cacttacgcg catctggcgc gcgtcggcga caaggcgatc ctgaccagcc ccgacggcga
      181 cggcttcctg atgtacgcag tgcagggccg ggcctggatt gcgatgggcg acccgctcgg
      241 cacgccggcc gagcgccgcg aactggcctg gcggctgcgc gagcgcgccg agacctgggc
      301 gggctggacg gtgttctatc aggcgagccc ggcgcatctc gacctgtacg tcgagctggg
      361 cctgagcctg ctgaagatcg gcgaggaggc acgcgtcgag ctttcgacct tcacgctcga
      421 tggtgccgcg cgcaagaacc tgcgcaacac ccgcaaccgc ctcgtgcgcg acggctatgg
      481 tttccgcgtc gtgccggccg ccgaagtgcc ggcgctgctc gggcagctgc aggcggtttc
      541 cgatgcctgg ctcgatggca agcagacgcg cgagaagggc ttctcgctcg ggcgcttcga
      601 ggccggctat ctcggcggcg ggccggtggc ggtgatcgag cacgccgggc gcatcgtcgc
      661 gttcgcgaac ctctggtgcg gggcgcagca cgccgagctg tcggtcgatc tgatgcgctt
      721 cgccgacgat gcgccggccg gggcgatgga tgcgctgttc gtcgagctgc tgctgtgggg
      781 caaggccgaa ggctacgcct ggttcaacct cggcatggca ccgctcgccg gcctgcacgc
      841 ccgggcgctg gcgccgctgt ggaaccgctt cggcgcgctg gtgttcggcc gcggcgaacg
      901 cttctacaac ttccgcggcg tgcgccagta caaggaaaaa ttcggtcccg tctgggaacc
      961 gcgctatctc gccgcccccg gcggtctgcg cctgccgcgc gtgctcgcgg cggtgacgac
     1021 gctgatcgcc ggcggcctga gcggagtcgt ccagaaatga agctgtgccg tccnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgcgct gttctgcacc gtcgcctgct
     1201 cgacgctgac cccggccacg gccgaaaccc tgaactacgg gcgcttcggc gacatcgccg
     1261 tgctgcgccc ggagggcaag ccgcgctcgg tgaccattgt cgtctccggc agcggcgggc
     1321 cgatgcaggc cgaggccgag atcggccggc tgctgaacgc gcagggctcg ctggtgctgt
     1381 cggtcgatgg cgagcggctg ctgcacaaca tccgcccgaa accggacgag acctgcgccg
     1441 atgccggctg ggatttcgag gcgatgtcgc agtacgtgca gaaggaactc ggcctcggcg
     1501 actaccgccg gccgctgctg gtcggcgtcg ggctcggcgc atcgctggtg tttctgggcc
     1561 tcgccgaatc gacgccgaac accttcctcg gcggcatggc gctcggcttc gatccggact
     1621 ggcgcagccc gtggcggctg tgcgaaggcg atcagggcga ggtcggcaag ccggccgccg
     1681 acggccgcgg ctggcagctc gcgccgagcg acgaatacct gctgcgctgg accgtgctgc
     1741 agggcgcggc cgatccgtac gtcgccggcg cgaaggtcgc gcagttcgtc gagggcgtac
     1801 cgaatgccga gctggtgacg gtgcccggcg tcggccacgg cttcgccgag ccggcgcgct
     1861 ggcgcgattc cttcgtcgat gcgtggaagc gcctgaacaa cgatccctat caggaccgcg
     1921 agctgaccga gctgccgctg atcgagctgc cggtggcgca gccgtccggc gactggctgg
     1981 cggtgatcct cagcggcgac ggcggctggg cggcgatcga ccgcgaactc ggcgagacct
     2041 tccagcacga gggcatcccg gtggtcggct tcgacatgct gcgctacctg tggaaggcgc
     2101 gcagccccga ggaaaccgcc aacgccgtcg ccagcgcgat cgagcgctac acgcagcgct
     2161 ggcagcgccg gcgcgtgctg ctggtcggct actcgctcgg cggcgacgtg ctgcccttcg
     2221 tcgtcaaccg cctgccgccg cagacgcgcc agaagctggc gctgagcgcg gtgctggcgc
     2281 cgagcaaata cgtcgagttc gaattccatc cctgggactg gatctcggac gacgacgacg
     2341 cc
//
LOCUS       DAOQIE010000443         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_34487_length_2302_cov_5.330663, whole genome shotgun sequence.
ACCESSION   DAOQIE010000443 DAOQIE010000000
VERSION     DAOQIE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34487_length_2302_cov_5.330663"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK144_18020"
     CDS             complement(<1..1074)
                     /locus_tag="PK144_18020"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNF67673.1"
                     /translation="MSRSRGSLWRRLDRGRLLAMALWALPALSLLPLGVLWLWQQQVL
                     VFWLAATAGTGLLAWGLDRWLTARSRRLIGHAADGPDPDWPPSADAAWQQVEALAAGI
                     DPAEWPLDDANRWLELGRRTLDTVARHYHPQAERPLWELTVPHLLLIIERASRKLGHT
                     VVAHVPFSHQLTIGRVLALNRWRTLAGRAWNLYRAGRLVVNPANAVLGEVWTALRDRA
                     FGNAQQELQGWLLREYVLETGRYAIELYSGRLRLDESPDLPSAPDLVVTEQRAQAPVE
                     PLRILVLGQTNAGKSSLVNALFDLGEAAADGAHALVDALPATGAVTPYRLTRAGLTAA
                     LVFDTPGLDGLDAGDEGLNEAIAS"
     gene            complement(1071..>2302)
                     /locus_tag="PK144_18025"
     CDS             complement(1071..>2302)
                     /locus_tag="PK144_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNF67674.1"
                     /translation="LGAQWQALVDALAEPKADAAALAEALAEARSRQPAPVVWLLGRA
                     QTGKTSIIRALTGSPNAVIGDGFRPCTRTAQLYDFPATAPVVHFLDTRGLGEVAYDPS
                     EDMAWCEAQAHLVLAVVRALEPAHGPVFEALHAIRERHPDWPVLLAQTTLHEGYPPGA
                     PHVLPYPYERSPLSPAVPEDLARALAAQRAALDALPGPAVLTVPLDFTLPDDGFEPAD
                     YGLPALWDAIETALPAGLHALLAADPEVRDLLARTAQPHIRAWALASAALGALPAAGA
                     VAVPAAQAKLLHNLATLYGLTLDRRLVGEFLGALGAGVGAGYLARWAGREIAKLIPGW
                     GQTVGAAWGAATAGASTWALGEAAAYWFGEHRQGRPGDPELLRRHYAEALARGTRFLE
                     QRALPPASGDHDAGTPR"
BASE COUNT          308 a          882 c          849 g          263 t
ORIGIN      
        1 gctggcgatg gcctcgttca ggccctcgtc accggcatcg aggccgtcga gcccgggcgt
       61 gtcgaacacc agcgccgcgg tcagcccggc gcgcgtcagc cggtacggcg tcacggcgcc
      121 ggtcgccggc agcgcatcga ccagcgcgtg cgcgccatcg gccgccgcct cgccgagatc
      181 gaacagcgcg ttgaccagac tcgacttgcc ggcgttggtc tggccgagca cgaggatgcg
      241 cagcggctcg accggcgcct gcgcgcgctg ctcggtcacg acgagatccg gtgcgctcgg
      301 cagatcgggc gattcgtcga gccgcagccg gccgctgtac agctcgatcg cgtagcggcc
      361 ggtttcgagc acgtactcgc gcagcagcca gccctgcagc tcctgctgcg cgttgccgaa
      421 ggcgcggtcg cgcagcgccg tccacacctc gccgagcacg gcgttggccg gattcacgac
      481 cagacgcccg gcccggtaca ggttccacgc ccggccggcc agcgtgcgcc agcggttcag
      541 cgccagcacg cggccgatgg tgagctgatg gctgaacggc acatgcgcga ccaccgtgtg
      601 cccgagcttg cggctcgcgc gctcgatgat cagcagcaga tgcggcacgg tcagctccca
      661 cagcggccgt tcggcctgcg ggtgatagtg gcgggcgacg gtatcgagcg tgcgccggcc
      721 gagttccagc cagcggttgg cgtcatcgag cggccattcg gccgggtcga tgccggcggc
      781 gagcgcctcg acctgctgcc acgcggcatc ggccgacggc ggccagtcgg ggtccggacc
      841 gtccgcggcg tggccgatca gccggcggct gcgcgccgtc agccagcggt cgagccccca
      901 ggcgagcaac ccggtgccgg ccgtggcggc gagccagaac accagcacct gctgctgcca
      961 gagccacagc acgccgagcg gcagcagcga cagcgccggc agcgcccaca gcgccatcgc
     1021 cagcagccgg ccgcgatcga gccggcgcca cagcgagccc cggctgcggc tcatcgcggc
     1081 gtgcccgcgt cgtggtcgcc ggaggccggc ggcagcgcgc gctgttcgag gaagcgcgtg
     1141 ccgcgcgcca gcgcctcggc gtaatggcgg cgcagcagct ccggatcgcc gggccggccc
     1201 tggcggtgct cgccgaacca gtaggccgcc gcctcgccga gcgcccaggt gctggccccg
     1261 gccgtcgccg cgccccaggc ggcaccgacc gtctgtcccc agcccggaat cagcttggcg
     1321 atctcgcgcc cggcccagcg cgccagatac ccggcgccga ccccggcgcc gagtgcgccg
     1381 aggaactcgc cgaccaggcg ccggtcgagc gtcagcccgt acagcgtcgc gaggttgtgc
     1441 agcagcttcg cctgcgccgc cggcaccgcc accgcgccgg ccgccggcag cgcgccgagc
     1501 gcggcggagg ccagcgccca agcgcggatg tgcggctgcg ccgtgcgcgc gagcaggtcg
     1561 cgcacctcgg ggtcggcggc gagcagtgca tgcagtccgg ccggcagcgc ggtttcgatc
     1621 gcatcccaca gcgccggcag gccgtagtcg gcgggctcga agccgtcatc gggcagcgtg
     1681 aagtcgagtg gcaccgtcag gaccgccggc cccggcagcg catcgagcgc cgcacgctgc
     1741 gcggccagcg cccgggcgag gtcctcgggc acggccggcg acagcggcga gcgctcatac
     1801 ggatacggca gcacgtgcgg cgccccgggc ggatagccct cgtgcagcgt cgtctgcgcc
     1861 agcagcaccg gccagtccgg atgccgctcg cggatcgcgt gcagcgcctc gaacaccggc
     1921 ccgtgcgccg gctccagcgc ccgcacgacg gcgagcacca gatgcgcctg cgcttcgcac
     1981 caggccatgt cctcgctcgg gtcgtaggcg acctcgccga gtccgcgcgt atcgaggaag
     2041 tgcacgaccg gcgccgtcgc cggaaagtca taaagctgcg cggtgcgcgt acagggccgg
     2101 aagccgtcgc cgatcacggc gttcgggctg ccggtcagcg cgcggatgat cgaggtcttg
     2161 ccggtctgcg cgcggccgag cagccagacc accggcgccg gctgccggct gcgcgcctcg
     2221 gccagcgcct cggcgagcgc agcagcatcg gccttcggct cggcgagcgc atcgaccagc
     2281 gcctgccact gcgcgccgag cg
//
LOCUS       DAOQIE010000444         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_35512_length_2263_cov_6.013134, whole genome shotgun sequence.
ACCESSION   DAOQIE010000444 DAOQIE010000000
VERSION     DAOQIE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35512_length_2263_cov_6.013134"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK144_18030"
     CDS             <1..462
                     /locus_tag="PK144_18030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF67675.1"
                     /translation="LEPAGREAVVARLAARVRGLPGDLMMTHAQLRALHTAGMEIGGH
                     TISHPILTRTPAEVARAEIAGGRAELEALIDAPVRSFAYPNGKPGADYGPEHVAMVRE
                     LGFDCAVVTSHGVSVAATDPYQLARFTPWDRSESRFQLRMLRNSRRLHLAA"
     gene            complement(517..2208)
                     /locus_tag="PK144_18035"
     CDS             complement(517..2208)
                     /locus_tag="PK144_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNF67676.1"
                     /translation="MLCAEHPGALAHDEAGRCALLVGHPQWRDGALASVARRHGDAAA
                     ALAGWQRFGNGLPERLAGDFSLVIVDAPGGCVFAATDRIGRQPLYHAHCAGGLAFASS
                     AEALARLPGVDASIRPQAVHAYLYFHMVPSPLTIHAGIDKLPGAHRLEWRGADCDIAC
                     WWQPQFREETGADAGRLGREMRALIDNAVADLAAGHRCGVFLSGGLDSSTVAGVLARL
                     KPADADTFSIGFDVPGYDETGFARIAAGRFGTRQHVYYVSADDVLAAVQDISAHYDEP
                     FGNSSAIPAYYCARLAREAGVERLLAGDGGDELFAGNERYLKQKVFEHWLRLPPALRE
                     PLGAGLKALPAIGALGKAASYVRQAETPLPERLETYNFLHRIPAATMFEPDFLQAVDT
                     GWPLGWLRRLWTQAGEASALNRMMYLDWQRTLADNDLRKVERTCALAGVDVVYPLLDD
                     RLIEFSCRVPSALKLPGSRLRDFYKQAMRGFLPEEILNKSKHGFGLPFGIWMRSHAGL
                     RALAGDSLQKLARRGWLRPGFIDEALGLHGSGHAAYYGELVWVLMMLELWLDTHV"
BASE COUNT          325 a          808 c          832 g          298 t
ORIGIN      
        1 ctggagccgg ccgggcggga ggcggtggtc gcgcggctgg ccgcgcgcgt gcgtggcctg
       61 cccggcgacc tgatgatgac gcacgcccag ctgcgtgcgc tgcacacagc cggcatggaa
      121 atcggcggcc acacgatcag ccatccgatc ctgacgcgca cgccggcgga ggtggcgcgc
      181 gccgagatcg ccggcggtcg cgccgaactc gaagccctga tcgatgcccc ggtcaggagc
      241 ttcgcgtatc cgaacggcaa gcccggcgcc gactacggcc ccgagcacgt ggcgatggtg
      301 cgcgaactcg gcttcgactg cgccgtggtc accagccacg gcgtctcggt tgccgccacc
      361 gatccctatc agctcgcgcg cttcacgccc tgggaccgct ccgagtcccg cttccagctg
      421 cgcatgctgc gcaacagccg ccggctgcat ctggcggcct gaagccgcag tccccgcgcg
      481 gcccggcctg caccgggcgc gcgcgggatg ccgcgctcag acgtgggtgt ccagccacag
      541 ctccagcatc atcagcaccc acaccagctc gccgtaatag gcggcgtggc cgctgccgtg
      601 caggccgagc gcctcgtcga tgaagcccgg gcgcagccag ccgcggcgcg cgagcttctg
      661 caggctgtcg ccggcgagcg cgcgcaggcc ggcgtggctg cgcatccaga tgccgaacgg
      721 cagcccgaag ccgtgcttgg acttgttcag gatctcctcg ggcaggaagc cgcgcatcgc
      781 ctgcttgtag aagtcgcgca gccgcgagcc cggcagcttc agcgccgacg gcacccggca
      841 cgagaactcg atcagccggt catcgagcag cggatagacc acgtcgacgc cggcgagcgc
      901 gcaggtgcgc tcgaccttgc gcaggtcgtt gtcggcgagc gtgcgctgcc agtcgagata
      961 catcatccgg ttcagggcgc tggcctcgcc ggcctgcgtc cagagtctgc gcagccagcc
     1021 gagcggccag ccggtgtcga cggcctgcag gaagtccggc tcgaacatcg tcgccgccgg
     1081 gatgcggtgc aggaagttgt aggtttccag ccgctcgggc agcggcgttt cggcctggcg
     1141 cacgtagctg gcggccttgc cgagggcgcc gatcgccggc agcgccttca ggccggcccc
     1201 gagcggctcg cgcagcgccg gcggcagccg cagccagtgc tcgaacacct tctgcttcag
     1261 gtagcgctcg ttgccggcga agagctcatc gccgccatcg ccggcgagca gccgctcgac
     1321 gccggcctcg cgggcgagcc gcgcgcagta ataggccggg atcgccgagg agttgccgaa
     1381 gggttcgtcg tagtgcgccg agatgtcctg cacggcggcg agcacgtcgt cggcgctgac
     1441 gtagtacacg tgctggcgtg tgccgaagcg tccggcggcg atgcgcgcga agccggtctc
     1501 gtcgtagccc ggcacgtcga agccgatcga gaaggtgtcg gcatcggcgg gcttgagcct
     1561 ggcgagcacg ccggcgacgg tcgaggaatc gagtccgccg gacaggaaga ccccgcagcg
     1621 gtgcccggcg gcgagatcgg cgacggcgtt gtcgatcagc gcgcgcatct cccggccgag
     1681 gcggccggcg tcggcgccgg tctcctcgcg aaactgcggc tgccaccagc aggcgatgtc
     1741 gcagtccgcc ccgcgccact ccagccggtg cgcccccggc agcttgtcga tgccggcgtg
     1801 gatcgtcagc gggctcggca ccatgtggaa gtacaggtac gcatgcacgg cctgcggccg
     1861 gatcgaggca tcgacgcccg gcagtcgggc cagcgcctcg gcgctcgatg caaacgccag
     1921 cccgccggcg caatgcgcgt gatagagcgg ctggcggccg atgcggtcgg tggcggcgaa
     1981 cacgcagccg cccggcgcat cgacgatgac cagcgagaaa tcaccggcga gccgttcggg
     2041 cagcccgttg ccgaagcgtt gccagccggc gagtgccgcg gctgcgtcgc cgtggcggcg
     2101 ggccacggag gccagagcgc cgtcacgcca ctgcggatgt ccaacgagca gcgcgcagcg
     2161 tccggcctca tcgtgtgcca gggcgccggg atgctcggcg cagagcaccg tgccgggcgc
     2221 gcttcgcgtg gttccgggcg cgacgacgct gcctgcgggc gga
//
LOCUS       DAOQIE010000445         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_35855_length_2251_cov_7.223588, whole genome shotgun sequence.
ACCESSION   DAOQIE010000445 DAOQIE010000000
VERSION     DAOQIE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35855_length_2251_cov_7.223588"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..585
                     /gene="trmL"
                     /locus_tag="PK144_18040"
     CDS             121..585
                     /gene="trmL"
                     /locus_tag="PK144_18040"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNF67677.1"
                     /translation="MFHVVLFEPEIPPNTGNVIRLCANTGAELHLIHPLGFVLDDARL
                     RRAGLDYHEYARVREHASWPAFLEAVAPEHVYAFTTRGARPFHEPAYAPGDALLFGPE
                     TRGLPQALLESLPAGCRVRLPMRPGVRSLNLSNAVAIAVYEAWRQHGYAGGG"
     gene            683..>2251
                     /locus_tag="PK144_18045"
     CDS             683..>2251
                     /locus_tag="PK144_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67678.1"
                     /translation="MLSRLLHRAVSLLGPLLFLCALWALHHQLSEYSWDDLVVYLEAL
                     SPHRLRLALALTVASYAVITAYDWLALQYLRKPLAGARVLFASFVSYAFSNSVGVSVL
                     SSGSLRLRLYTGWGLSAIEIAQIVLFSAVTLWLGLLVTAGGVLLAQPVDLAELLHLPA
                     LPHGPWLGALLLVPVAGWFALALLRREPLRVGDWTLPVVRAPLAARQLVIGAADWLLA
                     AAVLYALLPRVADLGYGHFLAVFLVAQIVALVSHVPGGLGVFEATVIALLAPWVEAGD
                     LLGGLFAFRAIYYLLPLALATVALGLYETRTLGARLGRQAGEALRVAAPAVPVLLPSG
                     LALMAAICGGVLLMSTVSPADEARLAWMHHVVPLPLLEVSHFLGAVIGVGLILLAQGL
                     QRRLDAAWLLSCLLLAAGALAALLKGGDYEEASLLALLLAALVPCRAHFYRRTRLAEE
                     RPSAGWLAVTAMAVVAALWLGWFSYKEIDYRHALWWQFQFDEDGEAARFLRTLPGLFA
                     LAAAVAVHQLLVVGH"
BASE COUNT          216 a          810 c          818 g          407 t
ORIGIN      
        1 ccgctgtttc ggttccacgt gaaacgctcc acgcgcccgc ggccgtcggc cgggctcgcc
       61 ggggcgggcg cgggcgccgt atgctcgcgc gcctgctccg cacgccagtc cgcacccgcc
      121 atgttccacg tcgtgctgtt cgaacccgaa atcccgccga acaccggcaa cgtcatccgg
      181 ctgtgcgcca acaccggcgc cgagctgcat ctgattcatc cgctcggctt tgtgctcgat
      241 gacgcccgcc tgcgccgcgc cgggctcgac tatcacgaat atgcgcgcgt gcgcgaacac
      301 gcctcatggc cggcgtttct ggaagccgtt gcgccggagc acgtctacgc cttcaccacc
      361 cgcggcgccc ggccgttcca cgagccggcc tacgcgcccg gcgatgcgct gctgttcggg
      421 ccggagacgc gcgggctgcc gcaggcgctg ctcgaatcgc tgccggcggg ctgtcgcgtg
      481 cgcctgccga tgcgccccgg cgtgcgcagc ctgaacctgt cgaacgccgt cgccatcgcc
      541 gtctacgaag cctggcgcca gcacggctac gccggcggcg gctgaagcgc acattgtctt
      601 aatccggcac tcaggcggat gacgccagcc ggcttacact gccgccatcc gcggcccggg
      661 cgccgtctcg ccgaacctca ccatgctctc ccgattgctg caccgcgccg tgtcgctgct
      721 cggcccgctg ctgttcctgt gcgcgctgtg ggcgctgcat caccagctca gtgaatattc
      781 gtgggatgac ctcgtcgtct atctcgaagc cctgtcgccg caccggctgc ggctggcgct
      841 ggcgctgacc gtcgcgagct acgccgtcat caccgcctac gactggctgg cgctgcaata
      901 cctgcgcaag cccctcgccg gggcgcgcgt gctgtttgcg tccttcgttt cctatgcgtt
      961 cagcaacagc gtcggcgtgt cggtgctcag ctccggctcg ctgcgcctgc ggctgtatac
     1021 cggctggggg ctgtcggcga tcgagattgc gcagatcgtg ctgttctcgg cggtgacgct
     1081 gtggctcggc ctgctggtga cggccggcgg cgtgctgctg gcgcagccgg tcgatctggc
     1141 cgagctgctg cacctgccgg cgctgccgca cgggccgtgg ctcggggcgc tgctgctcgt
     1201 accggtcgcc ggctggttcg cgctggcgct gctgcggcgc gagccgctgc gcgtcggcga
     1261 ctggacgctg ccggtcgtgc gcgcgccgct cgccgcgcgc cagctcgtga tcggcgcggc
     1321 cgactggctg ctggcggcgg ccgtgctgta tgcgctgctg ccgcgcgtcg ccgatctcgg
     1381 ctacggacac ttcctcgcgg tgttcctggt cgcgcagatc gtcgcgctgg tctcgcatgt
     1441 gccggggggc ttgggcgtgt tcgaggcgac ggtgatcgcg ctgctggcac cgtgggtcga
     1501 ggccggcgat ctgctcggcg ggctgttcgc gttccgcgcc atctattacc tgctgccgct
     1561 cgcgctcgcg accgtggcgc tcggcctgta cgagacgcgc acgctcggtg cccggctcgg
     1621 ccggcaggcc ggcgaggcgc tgcgcgtggc ggcgccggcg gtgccggtgc tgctgccgtc
     1681 ggggctggcg ctgatggcgg cgatctgcgg cggcgtgctg ctgatgtcga cggtcagccc
     1741 ggccgacgag gcgcgcctcg cgtggatgca tcacgtcgtg ccgctgccgc tgctggaggt
     1801 ctcgcacttc ctcggcgccg tgatcggcgt cggcctgatc ctgctcgcgc agggtctgca
     1861 gcggcggctg gatgcggcct ggctgctgtc ctgcctgctg cttgccgccg gtgcgctggc
     1921 ggcgctgctc aagggcggcg actacgagga agcctcgctg ctggcgctgc tgctggcggc
     1981 gctggtgccg tgccgcgcgc atttctaccg gcgcacgcgc ctcgccgagg agcgcccgag
     2041 cgccggctgg ctggcggtga cggcgatggc cgtggtcgcg gcgctgtggc tcggctggtt
     2101 ctcgtacaag gagatcgatt accggcatgc gctgtggtgg cagttccagt tcgacgagga
     2161 cggcgaggcc gcgcgcttcc tgcgcacgct gccgggcctg ttcgcgctgg ctgcggcggt
     2221 cgcggtgcac cagctgctgg tcgtgggcca c
//
LOCUS       DAOQIE010000446         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_36234_length_2238_cov_6.431058, whole genome shotgun sequence.
ACCESSION   DAOQIE010000446 DAOQIE010000000
VERSION     DAOQIE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36234_length_2238_cov_6.431058"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..2052)
                     /locus_tag="PK144_18050"
     CDS             complement(313..2052)
                     /locus_tag="PK144_18050"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNF67679.1"
                     /translation="MTARTSPAVLSLRIGQHSERGAKPANEDFHGMLVPEGQALQLKG
                     VAIAIADGMSSSEAARLAAEYAVKGFLNDYYATPDSWTVRKSGEKVLSALNRWLYAQS
                     TALRDPARGLVTTFSALVLKSTTAHVFHIGDSRIWQLRDQTLEPLTRDHHAFGSGGRS
                     WLNRALGIDLRLDIDYRAVPVEVGDRYLFTTDGIHEYLQPREIKRLLNEHSEDPDAAC
                     RALAAAAHAAGSPDNLTCQLICIDSLPAQDADSVFRQLAELPFPPELETGKHFEGYRI
                     EREIHASSTSQLYLAVDEQSGERVVLKTPSVNYEDDPAYLERFRHEEWVGRRLNSPHV
                     MRVIEPPRRRRHLYHVLEYLDGQTLRQWMQAHPQPTLAETRALLGQIARGLRAFHRLD
                     MLHLDLKPENIHIDREGRVRIIDFGSVKIGGIAETASPVERTDLVGTRDYTAPELLLG
                     ESATTRCDLFSFGVIAYELLTGHLPYGERLGRETDAKALAKLHYIPARLTRSDLPRWV
                     DGALEKSVRLDPRLRYTVETEFMHDLLHANTEFTGGPVPPLIERNPLGLWRTLALLSL
                     VLNLVLLFLLSRR"
BASE COUNT          315 a          733 c          831 g          359 t
ORIGIN      
        1 gggaatgccg cccgcccgga agcggtcccc gcagacgggt tcgcgggctt gcgcgggccg
       61 gcagggccgg cgcctgcggc tcgtgccgtc ctgccgcgcg gggcagggcg ggatgcccgg
      121 cgagccggcg tgccggcagt gcgctgccgc tggccggagt cgctgcagac ggtacgcggc
      181 tccggcagcc cgaccggaag tgcgacctcg atcgtcggcg aatcaggatt tcccggcgcc
      241 tggctgcaca ggtccggccg cggctcagga gagacgggcc ggaatcccgg ccgccgccgg
      301 gggttttgtg gctcagcgcc ggctcagcag gaacagcagc accagattca gcacaagcga
      361 cagcagtgcc agcgtccgcc agagcccgag cgggttgcgc tcgatcagcg gtggcaccgg
      421 gccgccggtg aactcggtgt tcgcgtgcag caggtcgtgc atgaactcgg tctcgaccgt
      481 gtagcgcagg cgcgggtcga ggcgcacgga tttctcgagt gcgccatcga cccagcgcgg
      541 cagatcgctg cgcgtcagac gggccgggat gtagtgcagc ttcgcgagcg ccttcgcgtc
      601 ggtctcgcgg ccgagccgct cgccgtaggg caggtggccg gtcagcagtt cgtaggcgat
      661 gacaccgaac gagaacaggt cgcagcgcgt cgtcgccgac tcgccgagca gcagctccgg
      721 cgcggtgtag tccctggtgc cgaccagatc ggtgcgctcg accggcgatg cggtctcggc
      781 gatgccgccg atcttcaccg agccgaaatc gatgatgcgc acccggccct cgcggtcgat
      841 gtggatgttc tcgggcttca ggtccagatg cagcatgtcc agccggtgaa aggcgcgcag
      901 gccgcgggcg atctgcccga gcagcgcgcg ggtttccgcg agcgtcggct gcgggtgcgc
      961 ctgcatccac tggcgcagcg tctggccgtc caggtattcg agcacgtgat acaggtgccg
     1021 gcgccggcgt ggcggctcga tcacgcgcat cacgtgcggg ctgttgaggc gacggccgac
     1081 ccattcctcg tgccggaaac gctccagata ggccgggtca tcctcgtagt tgacggacgg
     1141 ggtcttcagc accacgcgtt cgccgctctg ctcgtcgacg gccagataca gctggctggt
     1201 gctgctcgcg tggatctcgc gctcgatccg gtagccctcg aagtgcttgc cggtctccag
     1261 ttcgggcggg aacggcagct cggcgagctg gcggaacacc gagtcggcgt cctgcgccgg
     1321 cagcgagtcg atgcagatca gctggcaggt caggttgtcg gggctgccgg cggcgtgggc
     1381 agcggcagcc agtgcccggc aggcagcgtc cgggtcctcg gagtgttcgt tgagcaggcg
     1441 cttgatctcg cgcggctgca ggtattcgtg gatgccgtcg gtcgtgaaca ggtagcggtc
     1501 gccgacctcg accggcacgg cgcggtaatc gatgtcgagg cgcaggtcga tgccgagcgc
     1561 gcggttcagc cagctgcgcc cgccgctgcc gaaggcgtgg tgatcccggg tcagcggttc
     1621 gagggtctgg tcgcgcagct gccagatgcg gctgtcgccg atgtggaaga cgtgcgccgt
     1681 cgtcgatttc agcaccagcg cactgaaggt cgtgaccagg ccacgcgccg gatcgcgcag
     1741 cgccgtgctc tgcgcataca gccagcggtt cagcgccgac agcaccttct cgccggactt
     1801 gcgcaccgtc caggagtccg gcgtcgcgta gtaatcattg aggaagccct tgaccgcgta
     1861 ttcggccgca agccgcgccg cctcgctcga actcatgccg tcggcgatcg cgatcgccac
     1921 gcccttgagc tgcagcgcct ggccctcggg caccagcatg ccgtggaagt cctcgttggc
     1981 gggcttggcg ccgcgttcgg aatgctggcc gatacgcagg ctcaggacgg caggcgaggt
     2041 gcgggcggtc atggtggtcg gttcgggcgc tcggctggaa gggcggcttc ggacaagccg
     2101 acggggcgtg cagcgatgcc gcacaccccg tcctggctgc aggtgagcgg atcaggtcac
     2161 gtcgatcatc tgcacgctgc cgtccggcat gagcggtgcc tacggcaacg tcggcagcgt
     2221 cgtgttcctg accgtgct
//
LOCUS       DAOQIE010000447         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_36595_length_2225_cov_6.471889, whole genome shotgun sequence.
ACCESSION   DAOQIE010000447 DAOQIE010000000
VERSION     DAOQIE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36595_length_2225_cov_6.471889"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..>2225)
                     /locus_tag="PK144_18055"
     CDS             complement(61..>2225)
                     /locus_tag="PK144_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNF67680.1"
                     /translation="NRQNGSLMAHFEGVPEGLRGERSLVDVIRLRRPFVVVDEAHNSR
                     TELAFDMLARFRPGGVMELTATPDLERTPTNVLHSVSAAELKAEEMIKLPIVLETEPD
                     WQQCLADAIGRRDALHKLADEERRTGAAYLRPLVLIQAEARRAGVETLDAGRVRHELI
                     THHGIPAGEIVIATGEEKGLEQIDTDYRLGIADPSCPVKFVITQKALAEGWDCPFAYI
                     LVSMASLSSATAVEQLLGRVLRQPGASHRQARALNQSYAFVVSRNFAETAGALRDRLI
                     AGAGFERHEVTEFVTAARPEQARLDLEGHTGRVFYQPVSIQLSEKPDLRSVPKPVRDK
                     VSWDGKLNTLTIKTPLTEDDTEVLKTSVTSESAATAIIEAAGISRTTAIEFFQTPAEL
                     GERFRVPQLALRVQGELQLFDDPEVLDYPWDLSTYDAAPTADDLRALGAGLKVSEGGE
                     IDVDGESGKLISRFLPDLQRDLGLVYQPEHWDELRLATWLCRNLPEPSLTHAGKQAFV
                     AGWLTELLRREGFTLARANLQKFLIRTLLEARIRELRKQAVSLAFQQTLFGDDAASRV
                     AVTDQYSFEFHALAYAPPRDYDGRFGHFEFRAHFYGRIGDFDSREEFECACWLDIQAQ
                     RGRIRFWVRNLVRREGCSFFLQKADGRFYPDFLCQLPDMTDRPGPVLAIEYKGADRWT
                     GAEDDRLIGGLWANLSDERCRFVMVTDKQWDWIEEKMD"
BASE COUNT          393 a          726 c          688 g          418 t
ORIGIN      
        1 tcgcatcagt cgcatcaatc aggcccggcg tgcggagcaa gtgcgcacgc cgggcaccgc
       61 tcagtccatc ttttcctcga tccaatccca ttgcttgtcc gtgaccatca cgaaccggca
      121 tcgctcatcg gacaggttcg cccacaagcc gccgatcagc cggtcatcct ccgcgccagt
      181 ccagcggtcc gcacccttgt attcgatggc cagaaccggt ccgggccggt ctgtcatgtc
      241 gggcaactga catagaaaat ccggataaaa gcggccatcg gccttctgca ggaagaacga
      301 gcagccttcg cgccgcacga ggttgcggac ccagaaccgg atgcgtcccc gctgcgcctg
      361 aatgtccagc cagcaggcac attcgaattc ctccctgctg tcgaagtcac cgatgcgtcc
      421 gtaaaagtgc gcacggaatt cgaaatgtcc gaaacgaccg tcgtaatcgc gagggggcgc
      481 ataggccagc gcgtgaaatt caaagctgta ctgatcggtc actgcgaccc gcgaggccgc
      541 gtcatcgccg aacagcgtct gctggaatgc caggctgacg gcctgcttgc gcagctcacg
      601 gattcgcgcc tccagcaggg tgcggatcaa aaacttctgc aggttggccc gcgccagtgt
      661 gaagccctcc cggcgcaata attcggtcag ccagccggcc acgaaagcct gtttgccggc
      721 gtgggtgagg gagggctccg gcagattgcg gcataaccag gtggcgagac gcaattcgtc
      781 ccagtgttcg ggctggtaga ccagccccag atcgcgttgc agatcgggca ggaagcgcga
      841 gatgagcttg ccgctttcgc catcgacatc aatctcgccg ccttcggata ctttcaggcc
      901 cgcgcccagc gccctcagat cgtccgcggt cggtgcggcg tcgtaagtcg acaagtccca
      961 cgggtaatcc agcacctccg ggtcgtcaaa cagttgcagt tcgccctgca cgcgcaaggc
     1021 aagctgcggt acgcgaaaac gctcaccgag ttcggccggc gtctgaaaga attcgatggc
     1081 cgtggtgcgg ctgatcccgg cagcctcgat gatggccgtt gctgcggact cgctggtgac
     1141 cgacgtcttg agaacttccg tatcgtcttc ggtcagcggt gtcttgatgg tcagggtatt
     1201 gagcttgccg tcccagctca ccttgtcgcg caccggtttc ggcacgctcc tgagatcggg
     1261 cttttcggaa agctggatcg acaccggctg gtaaaatacg cgcccggtgt gtccttccag
     1321 gtccaggcgt gcctgttcgg gccgggcagc ggtgacgaac tccgttactt cgtgccgttc
     1381 aaagccggcg cctgcaatca gacgatcccg cagcgcgcct gccgtctcgg cgaagttgcg
     1441 cgataccacg aaggcatagg actgattcag ggctctggcc tgccgatggc tggcaccggg
     1501 ttgtcgcagg acccggccca gcaattgctc cactgccgtg gcggacgata gcgaggccat
     1561 gctcaccagg atataggcga agggacagtc ccagccctcg gccagtgcct tctgggtaat
     1621 gacgaatttc accggacagg acggatcggc aatgcccagc ctgtagtcgg tgtcgatctg
     1681 ttccagtcct ttttcctcgc cggtcgcaat cacgatctcc ccggccggaa tcccgtggtg
     1741 ggtgatcagt tcgtggcgca cccgcccggc atcgagtgtc tccacgccgg cgcggcgcgc
     1801 ttcggcctga atcagcacca gcgggcgcag atatgcggcg cccgtccggc gctcctcgtc
     1861 ggccagtttg tgcagggcat cacgccgacc gatggcatcg gccagacatt gctgccagtc
     1921 cggttcggtt tccagcacga tcggcagctt gatcatttct tccgccttca gctcggcggc
     1981 ggaaacgctg tgcagcacat tggtcggcgt gcgttccaga tcgggcgtgg cggtcagttc
     2041 catgacgccg ccggggcgga atcgcgccag catgtcgaag gccagttcgg tacggctgtt
     2101 gtgcgcctcg tccaccacga cgaacggccg gcgcaggcgg atcacgtcca ccagcgaccg
     2161 ctcgccgcgc aggccctccg gcacgccctc gaagtgggcc atgaggctgc cgttctgccg
     2221 gttca
//
LOCUS       DAOQIE010000448         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_38724_length_2154_cov_6.274893, whole genome shotgun sequence.
ACCESSION   DAOQIE010000448 DAOQIE010000000
VERSION     DAOQIE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38724_length_2154_cov_6.274893"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1228)
                     /gene="nadA"
                     /locus_tag="PK144_18060"
     CDS             complement(155..1228)
                     /gene="nadA"
                     /locus_tag="PK144_18060"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNF67681.1"
                     /translation="MNAAVPVRSHRLTPEQVPPTIVETLEAGERAELTARIRSLLKAQ
                     DATLVAHYYTSPELQELADATGGYVSDSLDMARFGTECPSKTLIVAGVRFMGETAKIL
                     NPEKRVLMPDLHAECSLDLGCPPDEFSAFCDAHPDREVVVYANTSAAVKARADWMATS
                     GIALDLVRHLHAQGKKILWAPDKHLGAWVRDQTGADVLLWDASCVVHEAFKADGLVQM
                     KLEHPDALVLVHPESPAEVIALADYVGSTTGIIKAAKELPAQKFIVATDNGIFHKMRA
                     AAPEKTFIEAPTGGRGATCVSCAHCPWMAMNGLRSLARVLERGDNEILVPEDIRERAV
                     RPIRRLLDFARTRNTVVLGNNDA"
     gene            complement(1264..1905)
                     /locus_tag="PK144_18065"
     CDS             complement(1264..1905)
                     /locus_tag="PK144_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF67682.1"
                     /translation="MPADTLPVSTDIVLFSIRDERLEVLLVREDAGQPWALPGGRAGR
                     EEDLDASALRHLAARTGVRGVWLEQLYTFGRPDRDPAQRVIAVAYYALVPIGQLEVGP
                     ADQHRDWFPVRALPRLALDHAAIVARALDRLVAKLDYSTIALQFMPARFTLSALQAVY
                     ETILGEALDKRNFRKRVLALACIEDTGELSREGHHRPARLYRVARPGTVEFIK"
BASE COUNT          320 a          714 c          786 g          334 t
ORIGIN      
        1 agcgggtttc ggcagcggca gatccgggcc gggccgggcc gggcggcgcg gcgtggcggc
       61 cggcagactt tcggactgcg cgatcagggc cttcagcgcc gccgcgcggg cgtgcgcggc
      121 gaggcggcgg ctggcgtccg gacgtggcat tggttcaggc gtcgttgttg ccgagcacga
      181 cggtgttgcg cgtgcgcgcg aagtcgagca gccggcggat cgggcgcacg gcgcgctcgc
      241 ggatgtcctc gggcacgagg atctcgttgt cgccgcgttc gagcacgcgc gcgagcgagc
      301 gcaggccgtt catcgccatc cacgggcagt gcgcacacga cacgcaggtc gcgccgcgcc
      361 cgccggtcgg ggcctcgatg aaggtcttct ccggcgccgc ggcgcgcatc ttgtggaaga
      421 tgccgttgtc ggtcgcgacg atgaacttct gcgccggcag ctccttcgcg gccttgatga
      481 tgccggtggt cgagccgacg taatcggcga gcgcgatgac ctcggccggc gattccggat
      541 gcacgagcac cagcgcgtcc ggatgttcga gcttcatctg caccagaccg tcggccttga
      601 aggcttcgtg cacgacgcag gaggcatccc agagcagcac gtcggcaccg gtctggtcac
      661 gcacccaggc accgaggtgc ttgtccggcg cccacaggat ctttttgccc tgcgcgtgca
      721 ggtggcggac cagatcgagc gcgatgcccg aggtcgccat ccagtcggcg cgcgccttca
      781 cggccgccga ggtgttggcg tagacgacga cctcacggtc cggatgcgca tcgcagaagg
      841 ccgagaactc gtcgggcggg cagccgagat cgagcgagca ctcggcgtgc aggtccggca
      901 tcagcacgcg cttctcggga ttcaggatct tcgcggtctc gcccatgaag cgcacgccgg
      961 cgacgatcag cgttttcgac gggcactcgg tgccgaagcg cgccatgtcg agcgaatccg
     1021 acacgtagcc gccggtggca tcggcgagtt cctgcagctc cggcgaggtg tagtagtgcg
     1081 cgaccagcgt cgcgtcctgc gccttcagca ggctcctgat gcgcgcggtc agctcggcgc
     1141 gctcgccggc ctccagcgtc tcgacgatgg tcggcggcac ctgctcgggc gtcagccggt
     1201 ggctgcggac aggaacggct gcgttcatgg ggtgggctcc gggcggccgc ggggcgggcc
     1261 gcatcacttg atgaattcga cggtgccggg tcgcgcgacc cgatacagcc gtgccggccg
     1321 gtggtgtccc tcgcgggaca gctcgccggt gtcttcgatg cacgcgagcg cgagcacgcg
     1381 cttgcggaag ttccgcttgt ccagcgcctc gccgaggatg gtctcgtaca ccgcctgcag
     1441 cgccgacagc gtgaagcgcg ccggcatgaa ctggagggcg atggtcgaat agtcgagctt
     1501 ggcgaccagc cgatccagcg cgcgcgcgac gatcgccgcg tgatcgagcg cgagccgcgg
     1561 cagcgcgcgc accgggaacc agtcgcggtg ctgatcggcc ggaccgacct cgagctggcc
     1621 gatcggcacc agcgcgtagt acgcgaccgc gatcacgcgc tgcgccgggt cgcgatccgg
     1681 ccggccgaag gtatacagct gttcgagcca gacgccgcgc acgccggtgc gggccgcgag
     1741 atgacgaagc gccgaggcat cgaggtcttc ctcgcggccc gcccgcccgc ccggcagcgc
     1801 ccagggctgg ccggcgtcct cgcgcacgag cagcacttcg agccgttcgt cgcggatcga
     1861 gaacagcacg atgtcggtgg aaacgggcag ggtatcggcg ggcatgggct tggtgtgcgc
     1921 aggacacgaa tagcttagtg tcttcaatac acccgatgca atcctgccgg gcacggggat
     1981 atcagacccg gacaggcaag cgcagcggat cgtggccggc agaagtcggg tcatgccgat
     2041 catgcgtgaa tgcgttgttg atcccccttg tgtcttgacg cccgacccct ctcgcgcaag
     2101 gggagagata ccgtctttca gtcccccgtc aagccgtcgg ctgccagttc ggat
//
LOCUS       DAOQIE010000449         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_39115_length_2143_cov_4.692529, whole genome shotgun sequence.
ACCESSION   DAOQIE010000449 DAOQIE010000000
VERSION     DAOQIE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39115_length_2143_cov_4.692529"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PK144_18070"
     CDS             complement(<1..1667)
                     /locus_tag="PK144_18070"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNF67683.1"
                     /translation="MGFASAKGVLPAVAGISFDVAAGETYALLGESGCGKSATALALM
                     RLLPAAGRVLGGEVFLHGRELLGLPETEMRALRGGGMAMIFQEPATSLNPVMTVGRQI
                     GEVLVRRSDLSDEARRAKAERLLAAVGIADAARRLDEYPFQMSGGMKQRVMIAMALAG
                     DPALLVADEPTTALDVTIQAQILDLLQDLQLERDMGMLLITHDLGVVARVAHRVGVMY
                     AGELVEEAPRDAFFSAPKHPYTQALFAALPDLSRRGARLATIAGQVPALDRMPAGCRF
                     AARCPHAWDRCRETAPEWKAVAGSGGHRVRCHLVESPSQAVVVPAAGAAAGRAGRPTK
                     DDGLLAVADLKVHFPIRRGFLQRTVGHVRAVDGVSLALRPGRTLALVGESGCGKTTVG
                     KALLQLIHPTDGSVLLDGREIAGLSRGAMRPLRRRMQMVFQDPYASLDPRMRVGEIIA
                     EGMQALGTVAAGDQGPAVARLLSQVGLDAGAAERYPHEFSGGQRQRIAIARALAVQPD
                     ILICDEPTSALDVSVQAQILNLLGDLQADLGLAYLFISHDLRVVRALA"
     gene            complement(1721..>2143)
                     /locus_tag="PK144_18075"
     CDS             complement(1721..>2143)
                     /locus_tag="PK144_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67684.1"
                     /translation="ALCFILGLTSWTGLARLLRGEVLKLRELEYVQAAQAFGVSDLRI
                     IARHLLPNLMHIVIIALVMDFSGLVLAEAVLSYVGIGVDPTMISFGTMINNARMELGR
                     EPVVWWSLVAAFAFMFAMVLAANLLADAVRDAFDPRMA"
BASE COUNT          384 a          750 c          709 g          300 t
ORIGIN      
        1 gccagcgcgc ggaccacgcg aaggtcgtga ctgatgaaga gataggccag cccgagatcc
       61 gcctgaagat cgccgagcag gttgagaatc tgcgcctgaa ccgagacatc gagcgccgag
      121 gtcggttcgt cacagatcag gatgtcgggc tgaacggcca gtgcacgtgc gatcgcgatg
      181 cgttgccgct gcccaccgga gaactcgtgc ggatagcgtt cggcggcgcc ggcatcgagt
      241 ccgacctgcg acagcaggcg ggcaacggcc ggcccctgat cgccagccgc caccgtgccg
      301 agcgcctgca tgccttcggc gatgatctcg ccgacacgca tgcgcgggtc gagcgaagcg
      361 tagggatcct gaaacaccat ctgcatgcgt cgccgcagcg ggcgcatggc gccgcgcgat
      421 aatccggcaa tctcgcggcc atcgagcagc acgctgccgt cagtgggatg aatcagttgc
      481 agcagcgcct tgccgactgt cgtcttgccg catccggatt cgccgaccag cgccagcgtg
      541 cgcccggggc gcagcgccag cgaaacgcca tcgacggccc gcacatgacc gaccgtacgc
      601 tgcaggaaac ctcgccggat cgggaaatgg accttcaggt cggcgaccgc cagcagcccg
      661 tcatccttcg tcgggcgccc ggcgcggccc gctgcggcac cggccgccgg caccacaacc
      721 gcctgcgatg gggattcgac gagatggcag cgcacgcgat ggcctccgct gcccgcgacc
      781 gccttccact cgggcgcggt ctcgcggcag cggtcccacg catgcgggca gcgcgcagcg
      841 aagcggcagc cggccggcat gcggtcaagc gccggcacct gaccggcaat cgtcgccagc
      901 cgggcaccgc gccgcgagag gtcgggcagc gctgcaaaca gcgcttgtgt atagggatgc
      961 ttcggcgccg agaagaaagc gtcgcgcggc gcttcctcga ccagttcgcc ggcgtacatg
     1021 acgcccaccc gatgcgcgac gcgggcgacg acaccgaggt cgtgcgtgat cagcagcatg
     1081 cccatgtcgc gctcgagctg caggtcctgc agcaggtcga ggatctgggc ctggatggtc
     1141 acgtcgagcg ccgtcgtcgg ctcatcggcc acgagcagcg ccgggtcgcc ggccagtgcc
     1201 atcgcgatca tcacgcgctg cttcatgccg ccggacatct ggaaggggta ttcgtcgagc
     1261 cggcgcgcgg catcggcgat gccgaccgcg gccagcagcc tctcggcctt cgcccgccgc
     1321 gcctcatcgg aaagatcgct gcgccgtacc agtacttcgc cgatctggcg accgacggtc
     1381 attaccgggt tgaggctggt ggccggctcc tggaagatca tcgccatgcc gccgccccgc
     1441 agtgcgcgca tctccgtttc cggcagtccg agcagctcac ggccgtggag gaaaacctcg
     1501 cccccgagca cgcgcccggc cgccggcagc aggcgcatca gcgccagcgc ggtggccgac
     1561 ttgccgcaac cggattcacc gagcaatgca tatgtctcgc cggcggcgac atcgaagctg
     1621 atgccggcca ctgccggcaa cacccccttc gccgaggcaa agcccatcgc cagatcacgt
     1681 accgaaagca gcggcatgcc cgtctccgta ggtttcgcca tcaggccatc ctcggatcga
     1741 aggcgtcgcg gacggcgtcg gcgagcaggt tggctgccag caccatggcg aacatgaagg
     1801 cgaaggccgc gaccagcgac caccagacga ccggttccct gcccagttcc atgcgcgcgt
     1861 tgttgatcat cgtgccgaaa ctgatcatgg tcggatcaac gccgatcccg acatacgaaa
     1921 gcacggcttc ggccagtacc agcccggaaa aatccatcac cagcgcgatg atgacgatat
     1981 gcatcaggtt cggcagcagg tggcgcgcga tgatgcgcag gtcggagaca ccgaaggcct
     2041 gtgccgcctg gacatactcc agttcgcgca gcttcagcac ttcgccgcgc aggaggcgcg
     2101 ccagtccggt ccagctggtc aggccgagga tgaagcagag cgc
//
LOCUS       DAOQIE010000450         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_39144_length_2142_cov_7.157643, whole genome shotgun sequence.
ACCESSION   DAOQIE010000450 DAOQIE010000000
VERSION     DAOQIE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39144_length_2142_cov_7.157643"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /gene="treY"
                     /locus_tag="PK144_18080"
     CDS             <1..2141
                     /gene="treY"
                     /locus_tag="PK144_18080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HNF67685.1"
                     /translation="AGFAQEAAGGGPLAEALAGLYARFAPDSAEGRQRLHYLLERQPY
                     RLAFWRTAADEINWRRFFDVSELAGVRVELPAVFERVHATTLRLYAEGLIDGVRVDHV
                     DGLADPRAYCRRLRRALAQAAKQRPADAPAGRAWLVVEKILAATEHLPADWQTDGTTG
                     YSFMNSVGALLHDPAGEAPLARLWSEVTGRSAQFEDEERAARRRIPKELLGADFNACA
                     HALHTIARSDPRTRDCTLLAIRRVLAELLVQFPVYRTYADARGRNAGDAALMQGVIAA
                     TAAQCRPADRWVLEHVDRWLGGEPPEAAPGITGRRLRLRAIGRFQQLSAPTAAKSVED
                     TAFYRHGKLLSRNEVGANPTQFALSPAEFHAEARARRRHFPDALLATATHDHKRGEDL
                     RARLAVLSELPEAWRQQLERWRLQNAPLRPAAGPDAADECMLYQMLVGIWPAGATPPD
                     AATLAVLRERLGGWQLKALREAKRHSHWSEPNLEYEAACADFLAALLDPARAADFLAG
                     LHGFAARIAPAGAVNSLLQTLLRLGTPGVPDLYQGCEFWDQSLVDPDNRRPVDWPARA
                     AALAGAATDAELLAAWPDGRLKQRLIARMLELRRADPALFARGDWRELAVLGRWSAQV
                     FAAVRVREGRCVLLAGLRLATGLLIGGEQSLVPPTAVWGDTRLKLPGRLAGLRWRSVL
                     DAGLPPLTGATIPAGALFARWPVAVLAGGG"
BASE COUNT          262 a          826 c          776 g          278 t
ORIGIN      
        1 aggccggctt cgcgcaggag gccgccggcg gcggaccgct cgccgaggcg ctggccgggc
       61 tgtacgcgcg cttcgcgccc gacagcgccg agggccgcca gcgtctgcat tacctgctcg
      121 aacgccagcc gtaccggctc gcgttctggc gcaccgccgc cgacgagatc aactggcggc
      181 gcttcttcga tgtcagcgag cttgccggcg tgcgcgtcga gctgccggcg gtgttcgagc
      241 gcgtgcatgc gaccacgctc aggctgtacg ccgagggcct gatcgacggc gtgcgcgtcg
      301 atcacgtcga cggcctcgcc gatccgcgcg cctactgccg gcgcctgcgt cgcgcgctcg
      361 cgcaggccgc gaagcagcgc ccggccgatg ccccggccgg gcgcgcatgg ctggtggtcg
      421 agaagattct ggctgcgacc gagcacctgc cggccgactg gcagaccgac ggcacgaccg
      481 gctacagctt catgaactcg gtcggggcgc tgctgcacga tccggccggc gaggcaccgc
      541 tcgcgcggct ctggagcgag gtcaccggcc gcagcgcgca gttcgaggat gaagagcgcg
      601 ccgcgcgccg gcgcatcccg aaggagctgc tcggcgccga cttcaacgcc tgcgcgcacg
      661 cgctgcacac gatcgcgcgc agcgatccgc gcacgcgcga ctgcacgctg ctcgcgatcc
      721 ggcgcgtgct cgccgagctg ctcgtgcagt tcccggtcta tcgcacctat gccgatgcgc
      781 gcggacgcaa cgccggcgat gcggcgctga tgcagggcgt catcgccgcc actgccgcgc
      841 agtgccggcc ggccgatcgc tgggtgctcg aacacgtcga ccgctggctc ggcggcgagc
      901 cgcccgaggc cgccccgggc atcaccggcc gccggctgcg gctgcgcgcg atcggccgct
      961 tccagcagct cagcgcaccg accgccgcca agtcggtcga ggacacggcc ttctaccgcc
     1021 acggcaagct gctgtcgcgc aacgaggtcg gcgccaaccc aacgcagttc gcgctgtcgc
     1081 cggccgaatt ccacgccgag gcacgcgcgc gccggcgcca tttccccgat gcgctgcttg
     1141 cgaccgcgac ccacgaccac aagcgcggcg aggacctgcg cgcgcggctg gcggtgctga
     1201 gcgagctgcc cgaggcctgg cgccagcagc tcgaacgctg gcgcctccag aacgcgccgc
     1261 tgcgcccggc tgccggtccc gatgccgccg acgaatgcat gctgtatcag atgctggtcg
     1321 gcatctggcc ggcgggcgcg acgccgcccg atgccgcgac gctcgccgtg ctgcgcgagc
     1381 gcctcggcgg ctggcagctg aaggcgctgc gcgaggccaa gcgtcacagc cactggagcg
     1441 agccgaacct cgaatacgag gccgcctgtg cggacttcct cgccgcgctg ctcgacccgg
     1501 cgcgcgccgc cgacttcctc gccggcctgc acggcttcgc cgcgcgcatc gccccggccg
     1561 gcgccgtcaa cagcctgctg cagacgctgc tgcgcctcgg cacgccgggc gtgcccgatc
     1621 tctaccaggg ctgcgagttc tgggaccaga gcctcgtcga ccccgacaac cgccgcccgg
     1681 tcgactggcc ggcgcgcgcc gccgccctcg ccggcgccgc aacggatgcc gaactgctcg
     1741 ccgcctggcc ggacggtcgc ctgaagcagc gcctgatcgc gcgcatgctc gaactgcgcc
     1801 gcgccgatcc ggcgctgttc gcacgcggcg actggcggga gcttgcggtg ctcgggcgct
     1861 ggtcggcaca ggtgttcgcg gccgtgcgcg tgcgggaagg ccgctgcgtg ctgctcgccg
     1921 gcctgcgcct ggcgaccggc ctgctgatcg gcggcgaaca gtcgctggtg ccgccgaccg
     1981 cggtctgggg cgacacgcgc ctgaagctgc cgggcagact cgccggcctg cgctggcgca
     2041 gcgtgctcga tgccggactg ccgccgctga ccggcgcgac gatcccggcc ggcgccttgt
     2101 tcgcgcgctg gccggtggcg gtgctggcgg gcggcggctg aa
//
LOCUS       DAOQIE010000451         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_40169_length_2111_cov_5.236868, whole genome shotgun sequence.
ACCESSION   DAOQIE010000451 DAOQIE010000000
VERSION     DAOQIE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40169_length_2111_cov_5.236868"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PK144_18085"
     CDS             <1..1283
                     /locus_tag="PK144_18085"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNF67686.1"
                     /translation="VGRTDLRPRIEALDRGGRQVLALIDACYSGNTLRGLYRNTLARP
                     AFTKRYVALPPRGAGSEPAAKDPDLCGYNCASAVEEAFPYRAVAYVSSASEGEAAVDL
                     GSSVLASVPTYDGRPHGAFSDALLRALRGEGPLLADRDGDGRLSAAELHQVTLNFLRQ
                     RGLPHTPQLLPRLEDDRSGIAAAIFLQGTPPPGTVPAAPAAGRTLRVQLVDATPALTT
                     VLQGLAQLTLVERDARLIVRRDGEEWQLQSAAGDEITRLPATGIKKVVAAVRAQAWVE
                     RVLAAPQDSGFRLELELSGQARGSVAVGGQSIHFAVRSAQPAQLLVLTVSTDGSVAVL
                     YPGNAAELQPVPAGTAVRIPSVDTESILVQAPFGTDQVLAFAFPHPEPRLRELIGGFW
                     ESGAPQLEALGDWLGDPALARAQLSLVTRPGPGS"
     gene            1304..>2111
                     /locus_tag="PK144_18090"
     CDS             1304..>2111
                     /locus_tag="PK144_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67687.1"
                     /translation="MTEFQSGSMKRVVAVLVGLGSLSLSGAFAEDCQRGEQMFEQSKT
                     EPQVSQRIVLLERSASLCGRFATHYALGRERLALGVTGPAAEAFETALQLAGDDRAAM
                     LALGRLAEARYEQGRIGEAVAALEGAYGRLAAVSPPQAAPPWLEALRRTLDVRMASTV
                     VTADTMRSIFTSFATRGIGVVPRVQLPVQFDYDSSELAAEGISQVTELGMALATPKLL
                     APQQKIRVVGHTDARGTDAYNDRLSLARAQTVARALEQRLPELAGRIVVEG"
BASE COUNT          317 a          687 c          735 g          372 t
ORIGIN      
        1 tggtcgggcg cacggacctg cgcccgcgca tcgaggcgct ggatcgtggc ggccggcagg
       61 tgctggcgct gatcgatgcc tgttattcgg gcaatacctt gcgcggcctg taccgcaata
      121 cgctggcacg gccagccttt accaagcgtt atgttgcgtt gccgccccgc ggtgccggca
      181 gcgaaccggc ggcgaaggac ccggacctct gcgggtacaa ctgcgccagt gctgttgaag
      241 aagcctttcc gtaccgggct gtggcctacg tatcctcggc cagcgaaggc gaggcggcgg
      301 tggatctcgg ctccagcgtg ctggccagcg tgcctaccta tgacggccgc ccgcacggag
      361 ccttcagcga tgccttgctg cgcgccctgc ggggggaggg accattgctc gccgaccggg
      421 acggggatgg ccggctcagt gctgccgaac tgcatcaggt gacgctgaat ttcctgcgcc
      481 agcgcggcct gccgcatacc ccgcagctgt tgccgcgcct cgaagacgat cggagcggca
      541 ttgccgctgc cattttcctg cagggaacgc cacctccggg tacggtgccc gctgcgcctg
      601 cggccggccg caccctgcgg gtccagctcg tcgacgcgac gccggccctg accacggttc
      661 ttcagggact ggcgcaactg acactcgtcg aacgcgatgc ccgcttgatc gtgcgccgtg
      721 atggagagga gtggcagctg cagtcagcgg ccggcgacga gattacccgg ctgccggcaa
      781 cgggcatcaa gaaggtcgtg gcagccgtgc gggcacaggc ctgggtcgag cgcgtgctgg
      841 cagcgccgca ggattcgggg ttccggctcg aactcgagct ttccggtcag gctcgcggca
      901 gtgtggccgt cggcgggcag tcgatccatt tcgcggtacg cagcgcgcag cccgcgcaac
      961 tgctggtact gaccgtatcc accgacggca gcgtcgcggt gttgtatccg ggcaatgccg
     1021 ccgaactgca gcccgtgccg gccgggacgg ccgtgcgcat tccgtcggtc gataccgaat
     1081 ccatcctggt acaggcgccg ttcggcactg atcaggtgct ggcctttgcg ttcccgcacc
     1141 ccgagccgcg attgcgggag ctgatcggcg gtttctggga gtccggtgca ccgcagctgg
     1201 aagccctggg cgactggctc ggtgatccgg ccctggcccg tgcgcagcta tcgctggtga
     1261 cccgtcccgg gcccggatcc tgaaccttct gccggaggca gccatgaccg aattccagtc
     1321 cggatccatg aagcgggtgg tggccgtgct tgtcgggctg ggcagtctga gtctttcagg
     1381 ggcttttgcc gaagactgcc agcgtggcga acaaatgttc gagcaatcga agaccgagcc
     1441 acaggtgtcg cagcggattg tgctgctgga gcgttcagcg tccctgtgtg gccgctttgc
     1501 cactcattat gccctcgggc gtgaacggct ggcgctcggt gtcaccggac cggcagccga
     1561 agccttcgag accgccctgc agctggccgg cgatgaccgc gctgcaatgc tggctctcgg
     1621 gcgactcgcc gaggcccgtt acgaacaggg ccggattggt gaggcggtgg cggcgctcga
     1681 aggtgcttat ggccggctgg cagccgtctc gccgccgcag gctgctccgc catggctcga
     1741 agcgttgcgc cgcacgctgg acgtacggat ggcaagtacc gtggtgaccg ccgatacgat
     1801 gcgcagcatc ttcacgagct tcgcgacgcg tggcatcgga gtggtcccgc gcgtgcagct
     1861 gccggtgcaa tttgactatg acagttccga gctggccgca gaaggcatca gccaggtcac
     1921 ggaactgggc atggcgctgg cgacgccgaa gttgctggca ccgcagcaga aaatccgtgt
     1981 cgtcggtcac accgatgcac gcggtaccga tgcctataat gaccgcctgt ccctggcccg
     2041 agcacagacg gttgcccgcg cccttgaaca gcggctgcct gaacttgccg gtcgcatcgt
     2101 tgtggaggga c
//
LOCUS       DAOQIE010000452         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_40436_length_2103_cov_5.312988, whole genome shotgun sequence.
ACCESSION   DAOQIE010000452 DAOQIE010000000
VERSION     DAOQIE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40436_length_2103_cov_5.312988"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK144_18095"
     CDS             <1..350
                     /locus_tag="PK144_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNF67688.1"
                     /translation="IGAAVSAVAGRLQAAAVVAYTSSGCSALRVARERPPAAVIGMTP
                     HAATARRLTLVWGVHAVLCADVTDVSGMSELACTTVRREGYGRPGQPVVIAAGMPFGT
                     AGSTNLLHIAELV"
     gene            416..1723
                     /gene="eno"
                     /locus_tag="PK144_18100"
     CDS             416..1723
                     /gene="eno"
                     /locus_tag="PK144_18100"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF67689.1"
                     /translation="MTTIQQVHGFEILDSRGNPTVAAQVVLADGSRGFAAAPSGASTG
                     SQEALELRDGDARRYLGRGVTRAVGHVNGEISRALGGRDAGDQTGIDRCLIELDGTPD
                     KSRLGANALLAVSLASARAAAAAADLPPYRCLNGAQTQFHLPVPMMNIVNGGVHAANS
                     IDLQEFMILPVGAASFAEALRWGTEVYHALKSRLARLGLATAVGDEGGFAPDLPSSEA
                     ALEQLMAAIEQAGFRPGTDIGLGLDLASTEFHHDGRYVLASEQRSFDAAGFVDHLAYL
                     ADSYPILSIEDGMAEDDHEGWRLLTARLGDRVQLVGDDLFVTNTRLLRRGIDAGLANA
                     ILIKPNQIGTLSETLAAIAMAEAAGYGAVISHRSGETEDTTIADLAVCTTATQIKTGA
                     PCRSDRVAKYNRLLLIEAELGAEARYRGRSAFCGMPRYPLAVD"
BASE COUNT          331 a          743 c          705 g          324 t
ORIGIN      
        1 ccatcggtgc cgcggtcagc gcggtggccg gccggctgca ggccgcggcc gtcgtcgcct
       61 acaccagctc gggctgttcg gcgctgcgcg tggcccgcga gcggccgccg gcagcagtga
      121 tcggcatgac cccgcacgcg gccacggccc gccggctgac gctggtctgg ggcgtgcacg
      181 ccgtgctctg cgccgatgtg accgacgtca gcggcatgag cgaactcgcc tgcacgacgg
      241 tgcgccgcga aggctacggc cggccggggc agccggtggt gatcgccgcc ggcatgccgt
      301 tcggcactgc cggtagcacc aacctgctgc acatcgccga actcgtctga gtctcggcct
      361 tgcggacctg atccgccacc gatccgaaac aggaacagaa acaggaacct tcagcatgac
      421 cacgatccag caggttcacg gcttcgaaat cctcgattcg cgcggcaatc ccaccgtcgc
      481 ggcccaggtc gtgctcgccg acggcagccg cggcttcgcc gccgcaccct ccggggcttc
      541 caccggctcg caagaggcgc tggagctgcg cgacggcgat gcccgccgct acctcggccg
      601 cggagtgacc cgcgcggtcg gtcacgtcaa cggcgagatc agccgcgccc tgggcggccg
      661 cgatgccggc gaccagaccg gcatcgaccg ctgcctgatc gagctcgacg gcacgcccga
      721 caagtcacgc ctcggtgcca acgcgctgct ggccgtgtcg ctggcgagcg cccgcgccgc
      781 ggccgccgct gcggatctgc caccgtatcg ctgcctgaac ggcgcacaga cgcagttcca
      841 tctgccggtg ccgatgatga acatcgtcaa cggcggcgtg catgccgcca acagcatcga
      901 cctgcaggaa ttcatgatcc tgccggtcgg agccgcgagc ttcgccgagg cgctgcgctg
      961 gggcacggag gtgtaccacg cgctgaagag ccggcttgcg cgcctcggcc tggcgacggc
     1021 ggtcggcgac gagggcggct tcgcgccgga cctgccttcg agcgaggccg cgctggagca
     1081 gctgatggcc gcgatcgaac aggccggctt ccggcccggt accgacatcg gccttggtct
     1141 tgacctcgcg agcaccgagt tccaccacga cggacgctac gtgctcgcct cggagcagcg
     1201 ctcgttcgat gcggccggct tcgtcgatca tctggcctat ctggccgaca gctatccgat
     1261 cctcagcatc gaggacggca tggccgagga cgaccacgag ggctggcgat tgctgaccgc
     1321 gcgcctcggc gatcgcgtgc agctggtcgg tgatgatctg ttcgtgacca acacacggct
     1381 gctgcgccgt ggcatcgacg caggccttgc caacgccatc ctgatcaagc cgaaccagat
     1441 cggcacgctg agcgaaaccc tggccgccat cgcgatggcc gaggcggccg gctacggcgc
     1501 cgtgatctcg catcgctccg gcgaaaccga ggacacgacg atcgccgatc tcgcagtctg
     1561 caccaccgcc acgcagatca agaccggcgc gccgtgtcgc agcgaccgcg tggccaaata
     1621 caaccgcctg ctgctgatcg aggccgaact cggtgctgag gcgcgttacc ggggccgcag
     1681 cgcgttctgc ggcatgcccc ggtatccgct ggccgtcgac tgagccgctg acgctgcggc
     1741 tgcgagccgt gcgcagcatt cccggtagcg ggccgcaggc atcggagcat gcctgcggtg
     1801 ccggcagcgg gcgcaggggc gggcaggccg gcactgcgcc tcatcttacg agtccggacg
     1861 atcattcacc cgctcgccga cgatttttat tctttcagaa tcatatgggt aatgattcaa
     1921 aacctggtcc ggaccgggcc ggagaagctg tccggaagct tgccggaact cgtgcctgcg
     1981 gcggcgacgg caggcgttaa aggcctttta tccttggttt ttcacccgct tgcgattttt
     2041 cgtactcgtc atacagctca gcgagccggg tgcgcaggaa atcggcgaaa ccacgctggc
     2101 cgg
//
LOCUS       DAOQIE010000453         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_42100_length_2056_cov_6.887056, whole genome shotgun sequence.
ACCESSION   DAOQIE010000453 DAOQIE010000000
VERSION     DAOQIE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42100_length_2056_cov_6.887056"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK144_18105"
     CDS             <1..662
                     /locus_tag="PK144_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNF67690.1"
                     /translation="TFGLVYAFSENFVLPLSHDEVVHGKGSLIARMPGDAWQQFANLR
                     AYFGFMWAHPGKKLLFMGGEFAQGREWSHERSLDWHLLDIHWHAGVQRLVRELNRLYR
                     ALPALHARDHDPDGFAWLSADNAAQSVYVWLRRAPAQPPVLVVANFTPQTHFGWRVGV
                     PLGGGWREVLNSDAPEYAGSGQCNPGRLETEAVPMHGEAQSLVITVPPLACAWFVPEA
                     G"
     gene            665..>2056
                     /locus_tag="PK144_18110"
     CDS             665..>2056
                     /locus_tag="PK144_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF67691.1"
                     /translation="MAARAQPVPEAGRPWPLGATATAGGVNFALYSGVAEAVELCLFD
                     AHGEHETARWRLAGPGDGIWHGFVPKLGIGARYGYRVYGPYRPDAGLRCNAHKLLLDP
                     YARAFDRPLILRDEHCGYIAESGDDRSFDLRDSAPWLPKCIVTDPAEDAAGCTARPHT
                     AWADTLLYETHVRGFTIRHPGIPEAARGRFEGMAQPAVVDWLKALGVSAVELLPVHAF
                     THERALLRRNLRNYWGYNSFGFFAPHADYGTAASLRALVRRLHEAGIEVILDVVYNHT
                     AESDECGPTICWRGLDNAAYYRLHAGQPRLYVNHTGCGNTLDASSPPVIRLIADSLRH
                     WATSYGVDGFRFDLATVLGRETRPGGDEFAPGAGLLDVLAQDPLLSRLKHIAEPWDIG
                     PGGYRLGGFPPGWHEWNDRCRDTLRRFWRADAGVLPELAARLHGSAETFGARGPQAGI
                     NFITSHDGFTLADL"
BASE COUNT          282 a          778 c          702 g          294 t
ORIGIN      
        1 tgaccttcgg cctcgtctac gccttcagcg agaacttcgt gctgccgctg tcgcacgacg
       61 aggtcgtgca cggcaagggc tcgctgatcg cgcgcatgcc cggcgatgcc tggcagcagt
      121 tcgcgaacct gcgcgcctat ttcggcttca tgtgggcgca tccgggcaag aagctgctgt
      181 tcatgggcgg cgagttcgcg cagggccgcg aatggagcca cgagcgcagc ctcgactggc
      241 acctgctcga catccactgg cacgccggcg tgcagcggct cgtgcgcgag ctgaaccggc
      301 tgtaccgcgc gctgccggcg ctgcacgcgc gcgaccacga cccggacggc ttcgcgtggc
      361 tgagcgccga caacgccgca cagtcggtct acgtctggct ccggcgcgca ccggcgcagc
      421 cgccggtgct ggtcgtcgcg aacttcacgc cgcagacgca cttcggctgg cgcgtcggcg
      481 tgccgctcgg cggcggctgg cgcgaggtgc tgaacagcga tgcgcccgaa tacgccggca
      541 gcggccagtg caaccccggc cgcctcgaaa ccgaagccgt gccgatgcac ggcgaggcgc
      601 agtcgctggt gatcacggtg ccgccgctcg cctgcgcgtg gttcgtgccg gaggccggct
      661 gagcatggcg gcgcgtgcgc agcccgtgcc ggaagccggc cggccgtggc cgcttggcgc
      721 caccgccacc gccggcggcg tgaacttcgc gctgtactcg ggcgtcgccg aggccgtgga
      781 gctctgcctg ttcgatgcgc acggcgagca cgaaaccgca cgctggcgcc tcgccggccc
      841 gggcgacggc atctggcacg gcttcgtgcc gaagctcggc atcggcgcgc gctacggcta
      901 ccgcgtttac ggcccctatc gcccggacgc cggcttgcgc tgcaacgcgc acaagctgct
      961 gctcgatccg tacgcgcgcg ccttcgaccg gccgctgatc ctgcgcgacg agcactgcgg
     1021 ctacatcgcc gaaagcggcg atgaccgcag cttcgacctg cgcgacagcg cgccgtggct
     1081 gccgaagtgc atcgtcaccg atccggcgga ggatgccgcc ggctgcaccg cccgcccgca
     1141 caccgcctgg gccgacacgc tgctgtacga gacccacgtg cgcggcttca cgatccgcca
     1201 tccgggcatt cccgaagccg cacgcgggcg cttcgagggc atggcgcagc cggccgtcgt
     1261 cgactggctg aaggcgctcg gcgtcagcgc cgtcgagctg ctgccggtgc acgcgttcac
     1321 gcacgagcgc gcgctgctgc ggcgcaatct ccgcaattac tggggctaca acagcttcgg
     1381 cttcttcgcc ccgcacgccg attacggcac ggcggcatcg ctgcgcgcgc tggtgcgccg
     1441 cctgcacgag gccggcatcg aggtgatcct cgatgtcgtc tacaaccaca ccgccgaaag
     1501 cgacgaatgc gggccgacga tctgctggcg cggcctcgac aacgccgcct attaccggct
     1561 gcacgccggg cagccgcggc tctacgtcaa tcacaccggc tgcggcaaca cgctcgatgc
     1621 ctccagcccg ccggtgatcc gcctgatcgc cgacagcctg cgccactggg cgacgagcta
     1681 cggcgtcgac ggcttccgct tcgacctggc gacggtgctc gggcgcgaga cccgtcccgg
     1741 cggcgatgag ttcgcgcccg gagccggcct gctcgacgtg ctcgcgcagg acccgctgct
     1801 gtcgcgcctc aagcacatcg ccgagccctg ggacatcggc ccgggcggct accggctcgg
     1861 cggcttcccg cccggctggc acgaatggaa cgaccgctgc cgcgacacgc tgcgccgctt
     1921 ctggcgcgcc gatgccggcg tgctgccgga actcgccgcg cgcctgcacg gttcggccga
     1981 aaccttcggc gcgcgcggcc cgcaggccgg catcaacttc atcaccagcc acgacggctt
     2041 cacgctcgcc gatctg
//
LOCUS       DAOQIE010000454         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_44396_length_1996_cov_2.754250, whole genome shotgun sequence.
ACCESSION   DAOQIE010000454 DAOQIE010000000
VERSION     DAOQIE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44396_length_1996_cov_2.754250"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK144_18115"
     CDS             complement(<1..262)
                     /locus_tag="PK144_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67692.1"
                     /translation="MSRPTLSGWIGLAPVNVRGERLDRSPSLLDGWLTALGARLRPGG
                     AECRRLRRFAEAVTTAAAGLAELDDAALTERCAGLRARLLRSG"
     gene            complement(255..>1996)
                     /locus_tag="PK144_18120"
     CDS             complement(255..>1996)
                     /locus_tag="PK144_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HNF67693.1"
                     /translation="PLWDCDRFLLATLPWLRGVFTRTGLLLWLAWVLPALFLAAGHWP
                     ALTGNLSDQLLGQQNLLLLALVFPVVKLLHELGHAYATRAAGGEVHEMGLMLLVFAPV
                     PYVDASAAGAIRSKHYRAFVGAAGMMTELALAAGALYLWLVLEPGLARSIAFNVMAVA
                     GFSTLVFNGNPLLRYDGYYILCDLAELPNLGQRSTQYWAWLARHHLFGERELPAPMAT
                     VGEKRWFLLYAPAALAYRCFVAISIALFIANQYFFVGVALALWSMTTMLGLPLVKMAG
                     HVFGHASRGRRLRTVSVSAGLCALLTGLVGWVPIPSSTVAEGVVWVPAQAELRAGGDG
                     WVDALSARQGEAVQTGQPVLQLQDEALQAELAERRARVRELEIRAIAELAEDRVQAAL
                     SREALTEARTALAETGQRVTALTLRAGSDGRLMLDRAADLPGRPLRRGDWIGYVLAGE
                     ALRTVRVTVGQDDIDRVRSALLTVSAMPADRIGTAYPVRVLRAVPAGEDRLPSRALAH
                     EGGGLHATDPRDEQGTRVLSRLFQFDLELPPEADGLRLGTRVYVRFGHPPEALATRLA
                     RRLRQLFLSQLHV"
BASE COUNT          338 a          708 c          710 g          240 t
ORIGIN      
        1 gaccgctgcg cagcaagcgg gcgcgcaggc cggcgcagcg ctccgtgagg gccgcatcat
       61 cgagctcggc cagtccggcg gccgcggttg tgacggcctc ggcgaagcgg cgcagacgcc
      121 ggcattccgc gccgcctgga cgcagccgcg caccgagcgc cgtcagccag ccatcgagca
      181 gcgatggcga tcgatcgagg cgttcgccgc gcacgttgac cggcgcgagg ccgatccagc
      241 cgctcagcgt cggcctagac atgaagctgc gacagaaaca gctggcgcag gcgacgcgcg
      301 agccgggtgg cgagtgcttc gggcgggtgt ccgaagcgca cgtagacgcg cgtgccgagc
      361 cgcaggccat ccgcttcggg cggcagttcc aggtcgaact ggaacagtcg cgacagcacg
      421 cgcgtaccct gctcgtcgcg cggatcggtg gcgtgcaggc caccgccctc gtgcgccagg
      481 gcccggctcg gcagccggtc ctcgccggcc ggcaccgcac gcagcacgcg caccggatag
      541 gccgtgccga tgcgatcggc cggcatcgcc gagaccgtca gcagcgcgct gcgcacgcgg
      601 tcgatgtcgt cctgtccgac cgtgacgcgc acggtgcgca gtgcctcgcc ggccagcacg
      661 tagccgatcc agtccccgcg gcgcagcgga cggccgggca gatcggcggc gcgatcgagc
      721 atcagccgac cgtcgctgcc ggcgcgcagc gtcagggccg tgacccgctg cccggtttcg
      781 gcgagtgcgg tgcgggcttc ggtcagggct tcgcggctca gcgccgcctg tacgcgatcc
      841 tcggccagtt cggcaatggc gcggatttcc agctcgcgca cccgcgcgcg acgttcggcc
      901 agctcggcct gcagggcctc gtcctgcagt tgcagcaccg gctggccggt ttgcacggcc
      961 tcgccctgcc gggcggacag ggcatcgacc cagccatccc cgccggcgcg caattcggcc
     1021 tgcgccggta cccagaccac gccttcggcg acggtcgagg acggtatcgg cacccagccg
     1081 accaggccgg tcagcagcgc acacaggccg gcactgaccg agaccgtgcg cagccgccgg
     1141 ccgcgggagg catgaccgaa cacatggccg gccatcttca cgagcggcag cccgagcatt
     1201 gttgtcatcg accacagcgc cagtgccaca ccgacgaaga agtactggtt ggcgatgaac
     1261 agcgcgatcg agatggcgac aaaacagcga taagccagcg cagccggagc gtaaagcagg
     1321 aaccagcgct tctcgccgac cgtcgccatc ggggctggca gctcgcgttc gccgaacagg
     1381 tgatggcgcg caagccaggc ccagtactgc gtgctgcgct ggccgaggtt cggcagctcg
     1441 gcgagatcgc agaggatgta gtagccgtcg tagcgcagca gcggattgcc gttgaacacc
     1501 agcgtcgaga agccggccac ggccatgacg ttgaaggcga tcgaacgcgc cagccccggt
     1561 tcgagcacga gccagagata cagcgccccg gccgcgagtg cgagttcggt catcatgccg
     1621 gcggcaccga cgaaggcgcg gtaatgcttg ctgcggatgg caccggcggc cgaggcatcg
     1681 acgtagggca ccggcgcgaa caccagcagc atcagcccca tctcgtgcac ctcgccgccg
     1741 gctgcccgtg tcgcataggc gtgaccgagt tcgtgcagca gcttcacgac cgggaacacc
     1801 agcgccagca gcagcaggtt ctgctggccg agcaactggt cggagagatt gccggtcagc
     1861 gccggccagt gtccggcggc gaggaacagc gccggcagca cccaggcgag ccacagcagc
     1921 aggccggtgc gcgtgaacac gccgcgcagc cacggcagcg tcgcgagcag gaagcggtcg
     1981 cagtcccaca gcggaa
//
LOCUS       DAOQIE010000455         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_45927_length_1959_cov_5.300945, whole genome shotgun sequence.
ACCESSION   DAOQIE010000455 DAOQIE010000000
VERSION     DAOQIE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45927_length_1959_cov_5.300945"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="hflK"
                     /locus_tag="PK144_18125"
     CDS             <1..748
                     /gene="hflK"
                     /locus_tag="PK144_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNF67694.1"
                     /translation="FTVQYRLKDARAFLFENRDPEEAVTLAAESAVREIVGRTAIDAV
                     LYEQRDAIAAELVKSIQNQLDRLNAGIVVANVNMQSVQPPEQVQAAFEDTLKAGQDGD
                     RLKKEGLAYASDVIPKAQGTAARLREEAEGYKAAVVAQAEGDAQRFSRVLTEYQKAPS
                     VTRDRLYIETMQQVYSNVSKVMIDSRSNSNLLYLPLDKLMQQTGGTAAAATPTGAAPA
                     AGDGSGSAASGTTLDPRSRDGQRGRDREGR"
     gene            771..1670
                     /gene="hflC"
                     /locus_tag="PK144_18130"
     CDS             771..1670
                     /gene="hflC"
                     /locus_tag="PK144_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNF67695.1"
                     /translation="MNRIGFLVGLVLLALMGAASTLFIVDQRQVAVIYALGEIKEVIT
                     EPGLKVKLPPPFQNVVFLDRRIQTLDSPDVRPIFTAEKKSLVIDWLVKWRVAEPRQFI
                     RNNGTDMRNLETRLAPVVHAAFNEEVTKRTVGGVLSTERDKVMQDVRARLVDDAKAFG
                     IEILDVRIKRVDFVANITESIYSRMRSERQQVANQLRSTGGAEGEKVRADADRQRQVI
                     VAEAYRDAQKVKGEGDARASAIFAEAFGRDPQFAEFYRSLEAYRASFRNKGDVMVIDP
                     SSEFFRAMRGSGPAPAAAAPAKK"
     gene            1677..1877
                     /locus_tag="PK144_18135"
     CDS             1677..1877
                     /locus_tag="PK144_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNF67696.1"
                     /translation="MGASAWDLLLAACALMLVLEGLLPFFSPGAWRRLFAQATKLSDG
                     QIRFLGLSSMLLGIVLLLLFRD"
BASE COUNT          325 a          676 c          681 g          277 t
ORIGIN      
        1 cttcaccgtg cagtaccggc tgaaggacgc gcgcgccttc ctgttcgaga accgcgaccc
       61 ggaagaggcg gtgacgctgg cggcggagtc ggcggtgcgc gagatcgtcg gccgcaccgc
      121 catcgacgcc gtgctctacg agcagcgcga cgcgatcgcc gccgagctcg tcaagtcgat
      181 ccagaaccag ctcgaccggc tcaacgccgg catcgtggtg gccaacgtca acatgcagag
      241 cgtgcagccg cccgagcagg tgcaggcggc gttcgaggac acgctcaagg ccggccagga
      301 cggtgaccgc ctgaagaagg aaggcctcgc ctacgccagc gacgtgatcc ccaaggcgca
      361 gggcacggcc gcgcgcctgc gcgaggaggc cgagggctac aaggccgcgg tggtggcgca
      421 ggccgagggt gatgcgcagc gcttcagccg cgtgctgacc gaataccaga aggcgccgtc
      481 ggtgacgcgc gaccgcctct acatcgagac catgcagcag gtctactcga acgtcagcaa
      541 ggtgatgatc gacagccgca gcaactccaa cctgctgtac ctgccgctgg acaagctgat
      601 gcagcagacc ggcggcacgg cggctgccgc gaccccgacg ggcgccgcgc cggccgcggg
      661 cgacggcagc ggcagcgccg cctcgggcac gacgctggat ccgcgctcgc gcgacggcca
      721 gcgcggccgt gaccgcgaag gccgctgatc gacacgggga gcatcagaac atgaaccgca
      781 tcggattcct cgtcggcctc gtgctgctcg cgctgatggg cgcggcctcg accctgttca
      841 tcgtcgacca gcgccaggtg gccgtcatct acgcgctcgg cgaaatcaag gaagtgatca
      901 ccgagcccgg cctgaaggtc aagctgccgc cgccgttcca gaacgtcgtc ttcctcgacc
      961 gccgcatcca gacgctggac agcccggacg tgcggccgat cttcaccgcc gagaagaaga
     1021 gcctggtgat cgactggctg gtgaagtggc gcgtcgccga gccgcgccag ttcatccgca
     1081 acaacggcac cgacatgcgc aacctcgaga cgcgcctggc gccggtggtg catgcggcgt
     1141 tcaacgagga ggtgaccaag cgcaccgtcg gcggcgtgct ctccaccgag cgcgacaagg
     1201 tcatgcagga cgtgcgcgcg cgcctggtcg atgacgccaa ggccttcggc atcgagatcc
     1261 tcgacgtgcg catcaagcgc gtcgacttcg tcgccaacat caccgagtcg atctacagcc
     1321 gcatgcgctc ggagcgccag caggtcgcca accagctgcg ctccaccggc ggcgccgagg
     1381 gcgagaaggt gcgtgccgat gccgaccgcc agcgccaggt gatcgtcgcc gaggcctacc
     1441 gcgacgcgca gaaggtcaag ggcgagggcg atgcgcgcgc ctcggcgatc ttcgccgagg
     1501 ccttcgggcg cgacccgcag ttcgccgagt tctaccgcag cctcgaggcc tatcgtgcca
     1561 gcttccgcaa caagggcgac gtgatggtga tcgacccgag cagcgagttc ttccgggcga
     1621 tgcgcggctc gggcccggcg ccggccgcag ccgcgccggc caagaagtag ccggcggtgg
     1681 gcgcgagcgc ctgggacctg ctgctcgccg cctgcgcgct gatgctggtg ctggaggggc
     1741 tgctgccctt cttcagcccg ggcgcgtggc gcaggctgtt cgcgcaggcg accaagctgt
     1801 cggacgggca gatccgcttt ctcggcctgt cgagcatgct gctcggcatc gtgctgctgc
     1861 tgttgttccg cgactgaacg gctctgcctg tcaggcaggc gccaggggtg cggccggtac
     1921 aatgcggttt ttaatcccgc gccgttttgc catgtcttc
//
LOCUS       DAOQIE010000456         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_47432_length_1925_cov_4.587166, whole genome shotgun sequence.
ACCESSION   DAOQIE010000456 DAOQIE010000000
VERSION     DAOQIE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47432_length_1925_cov_4.587166"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..761)
                     /locus_tag="PK144_18140"
     CDS             complement(63..761)
                     /locus_tag="PK144_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67697.1"
                     /translation="MSKHHDRNKMLNQILHAERLQQLLAPTEADLAEVEAMSEAELDE
                     ELAAAGIDYAAFAARLQQTLANHETPTLTPAPALPAGLAWLQRRGRRWLQATFEGLED
                     LRRGCAPMAPVLDAGLRETLPAALQAACLPVAVLSLHAVAGGVRLTVRWLAGAPDTAP
                     AVDVRLAGTPLVAEWLDWQASAGRTQSLLIRCALTAEMFDDHTGPLFAYDWCARQQQL
                     HIELLPEGDGDHGA"
     gene            complement(789..1289)
                     /locus_tag="PK144_18145"
     CDS             complement(789..1289)
                     /locus_tag="PK144_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67698.1"
                     /translation="MPTDLHQALAQMKPKDWKCLLALAGRKFGPAGLEAGELVNEAVA
                     LLLEGRRRWRPEVSIRTFLWQTVRSLGSHALERPARQRTQSLETCEIGVEIALADTAP
                     DADFEHDLACLRASLVDDPRLQVLLDGLLAGYSQTEIARQLELSNARVTALKQELIRR
                     LYRFFG"
BASE COUNT          311 a          607 c          647 g          360 t
ORIGIN      
        1 aggcctgcgt gccttcccaa aggaggtgct ggttcagggc gggtgccaaa ggattgatac
       61 cgtcatgcgc catggtcccc gtccccttcc ggcagcagtt cgatgtgtag ttgctgttgc
      121 ctggcgcacc agtcgtaggc gaacaggggg ccagtgtggt catcgaacat ttcggcggtg
      181 agcgcgcagc ggatcagcag actctgcgtg cgtccagcac tggcttgcca gtccagccat
      241 tcggcgacga gtggcgtgcc ggccagccgg acatcgaccg ccggggccgt gtccggcgcc
      301 ccggccagcc agcgcacggt cagacgcaca ccgccggcga cggcgtgcag cgacagcacg
      361 gccaccggca ggcaggccgc ctgcagggcc gccggcagcg tctcgcgcag gccggcatcg
      421 agcaccggcg ccattggcgc gcagccgcgg cgcaggtcct ccagaccttc gaacgtcgcc
      481 tgcagccagc gccggccgcg gcgctgcagc cacgcaagcc ctgcgggcag ggccggagcc
      541 ggggtcaggg tcggggtttc atgattcgcc agcgtctgct gcagccgtgc cgcgaacgcg
      601 gcgtagtcga tgccggcggc agcgagttcc tcatcgagtt cggcttcgct catcgcttcg
      661 acttcggcca gatcggcctc ggtcggggcc agcagttgct gcaggcgttc ggcgtgcagg
      721 atctggttca gcatcttgtt ccggtcgtgg tgcttgctca tggtctgcgt tgctcccgtg
      781 tggcgggttc agccgaaaaa acggtacagg cgccggatca gctcctgctt cagggccgtg
      841 acccgcgcgt tgctcagttc cagttgtcgg gcgatttcgg tctgcgagta accggcaagc
      901 aggccgtcga gcagcacctg caggcgcgga tcatcgacga ggctggcgcg gaggcaggcg
      961 aggtcgtgct cgaaatccgc atccggcgcc gtgtcggcca gcgcgatctc cacgccaatc
     1021 tcacaggttt cgagcgactg cgtgcgctga cgggcaggcc tttcgagggc gtgcgaaccg
     1081 agactgcgca cggtctgcca caggaacgtc cggatggaca cttccggccg ccagcggcgc
     1141 cggccttcga gcagcagcgc aacggcttcg ttgaccagtt ccccggcttc gaggccggca
     1201 ggaccgaact tgcgcccggc cagcgccagc aggcacttcc agtccttcgg cttcatctgg
     1261 gccagagcct gatgcaggtc ggtgggcatg gtgcgggttc tccgtgcggt gtccatgaat
     1321 ccattgccgg acagccgcgc ggattttcag cggacccgga aatttttttc cgcaggtccg
     1381 ctgcggccgg catcgctcag ccgaggtgtt ccagcgccag ccgggtcacg gtcagcacga
     1441 cgctcaggat gtgcagggcg gtcagggtga ggtcgagaag atgcatggcg gcggtctccg
     1501 cggggcgtgt ccggcgcgga ttgcgccgtc ctgaccatga atgccggctt gccgcgggat
     1561 ttttcaggcg gccattccct ggctgcggct ggccggtgcc acccggtcgg ctacgggttt
     1621 cgagacgatg cacagggctc ggtgtccgga tcggcattgc gcctcatctt acgaacccgg
     1681 aagtcccctc atctgcctgg atgacattca tattgtttat gaatcagaca catatgaatc
     1741 agaaagcacc atgtggcgat ggctgaaaag ctgtccgcac gctttccagc ccgtgtgaag
     1801 aaatggtcaa agttcttcgt taaatgattt ttatccttta ttatcaataa cttgcgattt
     1861 ttcgtactca tcatacagct cagcgagccg ggtgcgcagg aaatcggcga aaccacgctg
     1921 gccgg
//
LOCUS       DAOQIE010000457         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_49353_length_1883_cov_6.636214, whole genome shotgun sequence.
ACCESSION   DAOQIE010000457 DAOQIE010000000
VERSION     DAOQIE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49353_length_1883_cov_6.636214"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK144_18150"
     CDS             complement(<1..972)
                     /locus_tag="PK144_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67699.1"
                     /translation="MPVTLDLLSPAPATGGAALPSSPATTTSVDAGMSFGRALADARR
                     PMPQPDTAGSGDGHAQETKAEAAPSVAVQPAGRTDREAGAKAAADPAVDAVEETEPVE
                     APLAGASTDAGEPVDDDTAGKDDLLSGSPDTAGDPRTRAAAEALVELGLIPPPPQQQP
                     AIAEAPAAAIAAAITAGESGSDPLVRRALPTLTRMASAVPVTAGSLPGRGPDAPAADE
                     GSAAASVTGEASPQSAAAPTRHAHMAAAEGLRVPAAVAVPEAPVAGDARAATAGAAAG
                     AALPTGAAASMPDVQASGGTEPETAAVSMLGMPAAATWLKLLERRKPS"
     gene            complement(1043..1495)
                     /gene="fliJ"
                     /locus_tag="PK144_18155"
     CDS             complement(1043..1495)
                     /gene="fliJ"
                     /locus_tag="PK144_18155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNF67700.1"
                     /translation="MHDRIRQLRPAVDLAHEREDEAARLYRTIVGRRDEAKARLEQLR
                     QFRAEYLTRFHDDGRSGLSARRVQDYHAFIAGLDHSVEQTCRQLEQYEIEIQQLHKRW
                     LATRARTQALESVVERCRSEQRRVEDRREQAATDEFAAARHRSTRRPH"
     gene            complement(1539..>1883)
                     /gene="fliI"
                     /locus_tag="PK144_18160"
     CDS             complement(1539..>1883)
                     /gene="fliI"
                     /locus_tag="PK144_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003086458.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HNF67701.1"
                     /translation="ARGILDGHIVLSRRLAEGGQYPAIDIEASISRVMPQVTDARQYR
                     LARRFKELWANYMQHRDLISVGAYTRGSNPAVDQAIALYPRLREFLMQGMDEQVRYAD
                     GLAQLAAVLGES"
BASE COUNT          231 a          684 c          685 g          283 t
ORIGIN      
        1 agacggcttt cttcgttcga gcaacttcaa ccaggtggca gccgccggca ttccgagcat
       61 cgagactgcg gcggtttccg gctccgttcc tcccgacgcc tgcacatccg gcatcgatgc
      121 agctgcgccc gtcggcagcg cagcaccggc tgcagcaccc gcggtcgccg ctctggcgtc
      181 gccagccacc ggcgcctccg gcaccgccac ggcggcgggc acccgcaagc cttcggccgc
      241 tgccatgtgt gcgtgcctcg tcggcgccgc cgcggactgc ggcgaagcct cgccagtaac
      301 cgacgccgct gcactccctt catccgcagc cggcgcgtcc ggtccgcggc ccggcaagct
      361 gccggcagtc acgggcacgg cgctcgccat ccgcgtcagg gtcggcagcg cccgtcgcac
      421 cagcggatcg ctgccagact ccccggctgt aatcgcggct gcaatcgcgg ctgcgggcgc
      481 ctcggcgatc gctggctgct gctgcggcgg cggtggaatc agaccgagtt cgacgagcgc
      541 ctcggcagcg gcccgggtcc gcggatcacc ggcggtatcc gggctgccgg acaggagatc
      601 gtccttgccg gccgtgtcgt catcgaccgg ctcgccggca tcggtggacg ctccagccag
      661 gggcgcttcg accggctccg tttcctcgac ggcatccacg gccggatcgg ctgcggcctt
      721 ggcgccggcc tcgcgatccg tacgtccggc cggctgcact gcgacagacg gtgcggcttc
      781 ggccttcgtc tcctgggcgt ggccatcacc ggagccggcc gtatccggct gcggcatcgg
      841 gcgccgcgca tcggcgagcg cacggccgaa gctcatgccg gcatcgacgg aggtcgtcgt
      901 tgccgggctg gatggcagcg cggccccccc cgtggcgggg gccgggctca gcagatcgag
      961 ggtgacgggc atcgggatgc tccgggcgcc ggacgggcgg cgcggtcacg gacgggacgg
     1021 aaagggacag gtcgggaccg ggtcagtgcg ggcggcgcgt gctgcggtgg cgcgcagcgg
     1081 cgaactcgtc ggtcgccgcc tgttcgcgcc ggtcctcgac gcggcgctgc tcgctgcgac
     1141 agcgctcgac gacgctctcc agcgcctgcg tgcgcgcccg ggtcgccagc cagcgcttgt
     1201 gcagctgctg gatctcgatc tcgtactgtt cgagctggcg gcaggtctgc tcgacgctgt
     1261 gatcgagtcc ggcgatgaag gcgtggtagt cctgcacgcg ccgcgccgac aggcccgagc
     1321 ggccgtcgtc gtggaagcgc gtcaggtatt cggcgcggaa ctgccgcagc tgttcgagcc
     1381 gcgccttcgc ctcgtcgcgc cgcccgacga tggtgcggta cagccgggcg gcctcgtcct
     1441 cgcgctcgtg ggcgagatcg acggccgggc gcagctggcg gatacggtcg tgcatcgtgg
     1501 ttcccctcgc ggacgcaggc ccctgcgccc gcactggatc aggattcgcc gagcacggcg
     1561 gcgagctgtg ccagaccatc ggcatagcgc acctgctcgt ccatcccctg catcaggaat
     1621 tcacgcagcc gcgggtacag cgcgatcgcc tggtcgacgg ccggattcga gccgcgggtg
     1681 taggcgccga cgctgatcag gtcgcggtgc tgcatgtagt tggcccacag ctccttgaaa
     1741 cggcgcgcca gccggtactg gcgcgcgtcc gtgacctgcg gcatcacgcg cgagatcgag
     1801 gcctcgatgt cgatcgccgg gtactggccg ccctcggcca gccggcgcga cagcacgatg
     1861 tggccgtcga ggatgccgcg cgc
//
LOCUS       DAOQIE010000458         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_51966_length_1830_cov_7.421972, whole genome shotgun sequence.
ACCESSION   DAOQIE010000458 DAOQIE010000000
VERSION     DAOQIE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51966_length_1830_cov_7.421972"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1161
                     /locus_tag="PK144_18165"
     CDS             190..1161
                     /locus_tag="PK144_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HNF67702.1"
                     /translation="MSELQIYRNAAGAVEVRLDGDSVWLRQEQMAVLFGRDRTVIGRH
                     IRNLFAEGELAPESNVQNLHIPGSDKPVAFYNLDVIISVGYRVKSPEGVRFRQWATGV
                     LREHLTRGYTLSRQRLETNARELEAALLLVRQTARLPELSADQGRGLVDIVSRYAQTF
                     ALLQRYDEGLLTEPATQAGGCLPTAAEARTALAELKAGLQARGEAGELFARERGNGLE
                     ALLGNLDQTAFGEPVYASVESKAAHLLYFVIKNHPFADGNKRSAAYLFVDFLHRNRRL
                     LDARGEPVINDIGLAALCLLVAESAPAQKETLIRLIMNMLAPVEAGK"
     gene            1270..>1830
                     /locus_tag="PK144_18170"
     CDS             1270..>1830
                     /locus_tag="PK144_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNF67703.1"
                     /translation="MDTPVPSGRAGRYMRQPTGYRAFIPAPLPPDPPLALHGELQSLL
                     SQADRALGRLDGSVLTLPNPDLFVFMYVRKEAVLSSQIEGTQSSLQDLLAAEAELFNE
                     HMPRDVDEVVNYVRAMRLGLTRLAELPVSVRLIREIHAELMQGVRGGRLTPGELRRSQ
                     NWIGPAGCTLATASFVPPPHEEVPQIL"
BASE COUNT          329 a          614 c          599 g          288 t
ORIGIN      
        1 gcgcgagggc attgactggt ggtttgcgac ctgagcggcc ggcacactcc gcgcccggcc
       61 gccgtccgtg ccggccgccc ttttccccgc cagccgctcc gaatcccgca tgaacccgca
      121 ggccctgtcg tcgtgcatcc ttgcaggcgg cgtcgcgaac agcgtgcgca tgtccggagg
      181 actggccgaa tgagcgaact gcagatctat cggaacgccg caggcgcggt ggaagtacgg
      241 ctcgatggcg acagcgtctg gctgcggcag gagcagatgg cggtgctgtt cgggcgggat
      301 cgcacggtga tcggccggca tatccgcaac ctgtttgccg agggcgaact ggcaccggaa
      361 agcaatgtgc aaaatctgca cattcccggc tccgacaagc cggtcgcgtt ttacaacctc
      421 gacgtcatca tctcggtcgg ctaccgggtc aagtcgccgg aaggcgtgcg cttccggcag
      481 tgggcgaccg gcgtgctgcg cgagcacctt acgcgcggct acaccctcag ccgccagcgg
      541 ctggagacca acgcccgcga gctggaagcg gcactgctgc tggtgcgcca aaccgcccgg
      601 ctgccggaac tgagcgccga tcagggccgc gggctggtcg acatcgtcag ccgctacgcg
      661 cagaccttcg cgctgctgca gcgctacgac gaaggcctgc tgaccgaacc ggcaacgcag
      721 gccggcggct gtctgccgac ggcggccgaa gcgcgcacgg cgctggcgga actgaaagcc
      781 ggactgcagg cccgcggtga ggccggcgaa ctgttcgcgc gcgaacgcgg caacggactg
      841 gaagccctgc tcggcaatct cgaccagacc gccttcggcg agccggtgta tgcgagcgtc
      901 gaatcgaaag ccgcgcacct gctgtatttc gtgatcaaga accacccgtt cgcggacggt
      961 aacaagcgca gcgcggcgta tctgttcgtg gatttcctgc accgcaaccg ccgcctgctc
     1021 gatgcgcgcg gcgagccggt catcaacgac atcgggctgg cagcgctgtg tctgctggta
     1081 gccgaatcgg caccggcgca gaaggaaacg ctgatccgcc tgatcatgaa catgctggcg
     1141 ccggtggagg ccgggaaatg agtttgccga gcgcaagcca gccttgatca aggcacttcc
     1201 tcgtcgccat gctaaacgca gactatcaca tgcagtcatc tgacccgcgt gggcaagcga
     1261 ggaatgcaca tggacacccc ggtgccgtcg ggccgcgcgg gccgctacat gcgtcagccg
     1321 accggttacc gtgcgttcat ccccgccccg ctgccacccg acccaccgct tgccctgcac
     1381 ggagaactgc aaagcctgct gtcccaggcc gatcgcgcgc tcggacgact ggacggctcg
     1441 gtgctcacgc tgccgaaccc cgatctgttc gtcttcatgt acgtgcgcaa ggaggccgta
     1501 ctgtcgagtc agatcgaagg cacgcaaagc tcgctgcaag acctgctggc ggccgaagcc
     1561 gaactgttca acgagcacat gccgcgtgac gtcgacgaag tcgtgaacta cgtacgcgcc
     1621 atgcggctcg gactgacacg actggccgag ctgcccgtat cggtacgact gattcgtgaa
     1681 atccatgccg aactcatgca gggcgtgcgc ggcggccggc tgacacccgg tgagctgcgt
     1741 cgcagtcaaa actggatcgg tccggcaggt tgcactctgg cgaccgcgag tttcgtgccg
     1801 cctccgcacg aggaggtgcc gcaaatcctc
//
LOCUS       DAOQIE010000459         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_60951_length_1676_cov_2.910549, whole genome shotgun sequence.
ACCESSION   DAOQIE010000459 DAOQIE010000000
VERSION     DAOQIE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60951_length_1676_cov_2.910549"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="PK144_18175"
     CDS             complement(<1..1516)
                     /locus_tag="PK144_18175"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNF67704.1"
                     /translation="MPGKVKVFISYSHQDERYKDALEKHLGVAADRLEVWSDRRIVAG
                     ADWLAEIKQALAGCRVALLLISADFLTSPFILGIEVPALLRRREKQGLRVIPVIVQHC
                     IWDEVGWLKPIQARPLDGVALADLSKARCNKVLADLAREVLRLSAGTQQRPARRAPGL
                     SEPEQPPVIDIDRLPAGTDGFFGRVHELSQLDRLWQRDRNAALCVLVAPGGTGKTALV
                     QRWLARLRARHWCGAQRVFGWSFYSQGQADDRQVSEEPFLAAALAFFGVSVGAALPAH
                     AKGERLADAIAGTRTLLILDGLEPLQDAHGRLHSAEGLKRLLGRLLDRGQPGLCVITT
                     RLTLDDLADHRRSDVNRDGNALQHVLDNLDAADAARLLYRIGVRRAGGGTIAESRAPI
                     DLEWLAAGEAFGCHALSLSVLGRYLTDAHQGDVRHWREVKLLDIDPAWLGDGRNVYGH
                     AFRAMQAYERWLASGPDQKGTQKLAVLRLLGLFDRPATPDCLRALLRAPVIDGLT"
BASE COUNT          242 a          610 c          589 g          235 t
ORIGIN      
        1 cggtcagccc gtcgatcacc ggcgcccgca gcagcgcgcg caggcagtcg ggcgttgccg
       61 ggcggtcgaa caggccgagc aggcgcagta cggcgagctt ctgcgtgcct ttctggtccg
      121 gaccgctcgc cagccagcgc tcgtaagcct gcatcgcccg gaaggcgtgg ccgtagacgt
      181 tgcggccgtc gccgagccag gccgggtcga tgtcgagcag cttcacctcg cgccagtggc
      241 gcacatcgcc ctgatgggcg tcggtcagat agcggccgag cacgctcagc gacagcgcgt
      301 ggcagccgaa cgcctcgccg gccgcgagcc attcgaggtc gatcggtgcg cggctttcgg
      361 cgatggtccc gccaccggca cggcgcacgc caatccggta cagcagccgc gccgcgtcgg
      421 cggcatcgag gttatcgagg acgtgctgca gggcgttgcc gtcgcggttg acatccgagc
      481 gccggtggtc ggccagatcg tcgagcgtga ggcgcgtcgt gatcacgcac agccccggct
      541 ggccacgatc gagcagccgg ccgagcaggc gcttcagacc ctcggcgctg tgcaggcggc
      601 cgtgggcgtc ctgcagcggc tccagcccgt cgaggatcag cagcgtgcgc gtgccggcga
      661 tcgcatcggc cagccgctcg cccttcgcat gcgccggcag cgccgcaccg acactgaccc
      721 cgaagaaggc cagcgccgcg gccagaaacg gctcttccga cacctgccgg tcatcggcct
      781 gcccctggct gtagaagctc cagccgaaca cgcgctgcgc gccgcaccag tgccgcgcgc
      841 gcaggcgcgc cagccagcgc tgcaccagcg ccgtcttgcc ggtgccgccg ggcgcgacca
      901 gcacgcacag cgccgcatta cggtcgcgct gccagagccg gtcgagctgc gagagttcgt
      961 gcacgcggcc aaagaagccg tcggtgccgg ccgggaggcg gtcgatgtcg atgacgggcg
     1021 gctgttccgg ctcggacagg cccggcgcgc gccgcgccgg gcgctgctgc gtgccggcgc
     1081 tcaggcgcag gacctcgcgc gccagatcgg ccagcacctt gttgcatctg gccttgctca
     1141 ggtcagcgag cgccacgccg tcgagcggtc gggcctggat cggcttcagc cagccgactt
     1201 catcccagat gcagtgctgg acgatgaccg gaatcacccg cagcccctgc ttctcgcgcc
     1261 gcctgagcag cgccggcact tcgatgccga ggatgaacgg cgaggtcagg aagtcggccg
     1321 agatcagcag cagcgccacg cgacagccgg cgagggcctg tttgatctcc gccagccagt
     1381 ccgcaccggc cacgatccgg cggtcgctcc agacttcaag ccggtcagcg gcgacgccca
     1441 ggtgtttctc cagcgcatcc ttgtagcgct cgtcctgatg gctgtagctg atgaatacct
     1501 tgaccttgcc cggcatcgcg tgcccccttc cttctgccgg ccgagcgtaa cggccggcgc
     1561 aggggcggga caatgcgggc gggcgcggac cggtcggcct cgcctcagtc gtcctgcacc
     1621 ggcggccagt gcatgaacag ctccggcagt tcgccgagcg cggcgatcgt ggtcac
//
LOCUS       DAOQIE010000460         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_61415_length_1669_cov_6.328377, whole genome shotgun sequence.
ACCESSION   DAOQIE010000460 DAOQIE010000000
VERSION     DAOQIE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61415_length_1669_cov_6.328377"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..877)
                     /locus_tag="PK144_18180"
     CDS             complement(2..877)
                     /locus_tag="PK144_18180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNF67705.1"
                     /translation="MLILACPDYAGHATRIAELLGVPCRLLDIHRFPDGESRVQVPLP
                     LPAHVVFVRTLHQPNAKLVELLLAAATGRRHGARRLTLVAPYLCYMRQDIEFTPGEAV
                     SQGIVGDFLGGLFDDVLTVDPHLHRIDRLEEAIPRGNPVTLSAAVPLARLIAARWPEA
                     LLIGPDGESEQWVANIAAAGGLECAISHKVRHGDRDVCVSLPQARLDGRTVVLIDDIA
                     STGRTLAEAAMQLKARGARAVHAAVTHALIDADSLAALHAAGVDTFLSSNSVPHPSNA
                     IELEPLLAEAVRALA"
     gene            1073..1342
                     /locus_tag="PK144_18185"
     CDS             1073..1342
                     /locus_tag="PK144_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNF67706.1"
                     /translation="MPIYEYRCEKCGHELEAMQKMADAPLSDCPECQEPALKKLISAA
                     GFRLSGGGWYETDFKSGNKKNIAKKDDTPACGNAGCGSASSCASA"
BASE COUNT          247 a          589 c          595 g          238 t
ORIGIN      
        1 ctcaggccag cgcgcgcacg gcctcggcca gcagcggttc gagttcgatc gcgttgctcg
       61 ggtgcggcac gctgttgctg ctgaggaagg tatcgacgcc ggccgcgtgc agcgccgcga
      121 ggctgtcggc atcgatcagc gcgtgtgtga ccgccgcatg cacggcccgc gccccccgcg
      181 ccttcagctg catcgcggcc tcggcgagcg tgcgaccggt gctggcgatg tcgtcgatca
      241 gcacgacggt gcgcccgtcg aggcgcgcct gcggcaggct cacgcacacg tcccgatcgc
      301 cgtggcggac cttgtgcgag atcgcgcact ccagcccgcc ggccgcggcg atgttcgcga
      361 cccactgctc ggattcgccg tccgggccga tcagcagcgc ctccggccag cgcgcggcga
      421 tcaggcgcgc gagcggcacg gcggcgctca gcgtcaccgg gttgccgcgc gggatggcct
      481 cttcgagccg gtcgatgcgg tgcaggtgcg gatcgacggt cagcacgtcg tcgaacagcc
      541 cgccgaggaa gtcaccgacg atgccctgac tgacggcctc gcccggtgtg aattcgatgt
      601 cctgacgcat gtagcagagg tacggcgcca ccagcgtcag ccggcgcgcg ccgtggcggc
      661 ggccggtggc ggcggcgagc agcagctcga cgagcttggc gttcggctgg tgcagcgtgc
      721 gcacgaacac cacgtgcgcc ggcagtggca gcggcacctg gacgcggctt tcaccgtccg
      781 ggaagcgatg gatgtcgagc agccggcagg ggacgccgag cagttcggcg atgcgcgtgg
      841 cgtgtccggc gtagtcggga caggcgagga tcagcacggg gggcggtctc cgggcgcagc
      901 cggcgtcctg tcttgttttt ggccgctgcg cccccagatg gcgggtctgc aagcgtactg
      961 cgctgcgccg aattctgcct agcgcggtgc aacatgactt tgcggtaaga ttccgcagct
     1021 tgcgcgcgac cgcccgctgc gcatcgcccg tacctgccga ggttgaccga ccatgccgat
     1081 ctacgaatac cgctgcgaaa agtgcggcca cgaactcgaa gccatgcaga agatggcgga
     1141 tgctccgctg agcgactgcc ccgaatgcca ggagccggcg ctgaagaagc tgatctcggc
     1201 ggccggtttc cgcctctccg gcggcggctg gtacgagacc gacttcaagt ccggcaacaa
     1261 gaagaacatc gccaagaagg acgacacccc ggcctgcggc aacgccggct gcggctcggc
     1321 cagcagctgc gcgagcgcct gagcgcgtcc gccgccggcg gcacccaccg gcagtgccgg
     1381 ccggcggcga cgacgactgc ttcgcccgac ggggccgtga caccggcgcg cgacttgcgc
     1441 gcccggcgca cggccccgta ggctttcgcc cctttccgcg cccctgcgcc cgcaacgcgt
     1501 gcgcctgcgg ccaactgcct gaatcgatgg aagtccgaag aatgatgcgc agccactatt
     1561 gcggccaggt caccgaggcc ctgctcggtc aggaagtcca actctgcggc tgggcgcacc
     1621 gccgccgcga ccacggcggc gtgatcttcg tcgacctgcg cgaccgcga
//
LOCUS       DAOQIE010000461         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_67385_length_1587_cov_3.338120, whole genome shotgun sequence.
ACCESSION   DAOQIE010000461 DAOQIE010000000
VERSION     DAOQIE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67385_length_1587_cov_3.338120"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PK144_18190"
     CDS             <1..325
                     /locus_tag="PK144_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67707.1"
                     /translation="TERAGEVLDTVVDAALAGNLLAAQLLLSRCIPPARGQAVPVELP
                     ALAVAGTLTEKADAVLAAVGAGELAADTGAQLLGALGTVAKIRETDELEARIRALEAR
                     DAPQP"
     gene            309..632
                     /locus_tag="PK144_18195"
     CDS             309..632
                     /locus_tag="PK144_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67708.1"
                     /translation="MRRSLDTRLQALEARAAWHAPLAAFGTGPVAERLALLLRPADPS
                     RAGDNAIVARLVDRLAEAGRDHAQPADVQAIVAELFPAPEHAEAARILTRLFEFPETP
                     HEHTT"
     gene            616..867
                     /locus_tag="PK144_18200"
     CDS             616..867
                     /locus_tag="PK144_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67709.1"
                     /translation="MSTRLDARIARLEATAAARPPNREAALRRLWELGVIPDENGEYC
                     PLPPPDPDAKPCDPALRARVLARLRQLGCLNEDDPNAAT"
     gene            854..1036
                     /locus_tag="PK144_18205"
     CDS             854..1036
                     /locus_tag="PK144_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67710.1"
                     /translation="MPRLDTRLRLLETTGRPAPPPGVAIVHTGETAAAAIARARTDPA
                     LADRALIVVPEKEPLR"
     gene            1033..1263
                     /locus_tag="PK144_18210"
     CDS             1033..1263
                     /locus_tag="PK144_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67711.1"
                     /translation="MSLQRRVAKLEASTGIGGPHGEALRPLLRLTGWPEERPIPDDCT
                     LDLNALLAAAAASPASSPMARLRAAQSMASAS"
     gene            complement(1299..>1587)
                     /locus_tag="PK144_18215"
     CDS             complement(1299..>1587)
                     /locus_tag="PK144_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67712.1"
                     /translation="DRQKLARNAESLRDAATRLHAVSEGVRTGTPIVVAPAQPRQPAL
                     VEPGHVMGTDGTLYSRDGNGNLTSGRDGRVLTPAGPHGYIDTRTGQFVPAQ"
BASE COUNT          228 a          587 c          540 g          232 t
ORIGIN      
        1 caccgaacgg gccggcgagg tgctggatac cgtcgtcgat gccgcgctcg ccggcaacct
       61 gctggccgcg caactgctgc tgtcgcgctg catcccgccc gcccgcggac aggccgtgcc
      121 ggtcgaactg ccggcgctcg ctgtggccgg cacgctgacc gaaaaggctg atgccgtgct
      181 cgctgcggtc ggcgccggcg agcttgccgc cgacaccggc gcgcaactgc tcggggcgct
      241 cgggaccgtg gcgaagatca gggaaaccga cgaactcgaa gcccgcatcc gggcactgga
      301 agcccgcgat gcgccgcagc cttgataccc ggctgcaggc actcgaagcg cgtgccgcct
      361 ggcatgcacc cctcgccgca ttcggcaccg gcccggtcgc cgaacggctg gcactgctgc
      421 tgaggccggc cgacccgagt cgcgccggcg acaacgccat cgtcgcgcgg ctggtggatc
      481 ggctcgccga ggccggtcgc gatcacgcgc aacctgccga cgtgcaggcc atcgtcgccg
      541 aactattccc agcgcccgag catgccgaag ccgcgcgcat cctcacccgc ctgttcgagt
      601 ttccggagac accccatgag cacacgactt gatgcccgta tcgcccggct cgaagcgaca
      661 gccgctgccc ggccccccaa ccgtgaggct gccttgcgca ggctgtggga actgggcgtg
      721 atcccggacg agaacggcga gtattgcccg ctgccgccgc ctgatccgga cgccaagccg
      781 tgcgatccgg cgctacgggc gcgcgtgctc gccagactgc gccaacttgg ctgcctgaac
      841 gaggacgacc cgaatgccgc gacttgatac ccgtctgcgt ctgctcgaaa ccaccggccg
      901 ccctgcgccg ccaccgggcg tcgccatcgt gcacaccggc gaaaccgctg ccgctgccat
      961 cgcccgagca cgcactgatc ccgccctggc cgaccgggcg ttgatcgtcg tgccggagaa
     1021 ggagccgctg cgatgagcct tcagcgtcgc gtcgcgaagc tcgaagcctc gaccggcatc
     1081 ggcgggccgc acggcgaagc cctgcgcccc ctgctgcgcc tgactggctg gccagaggag
     1141 cggccgattc cggatgactg cacgctggat ctgaatgcgc tgctcgccgc agccgctgcc
     1201 tcgccggcat cgtcaccgat ggcccggctc agggcggcgc agtcgatggc gagcgcgtcc
     1261 tgagcggggc cggatcgtcg acggtgcgag cccggcgatc actgcgccgg tacgaactgc
     1321 ccggtgcgcg tgtcgatgta gccgtgtggt ccggcaggcg tcagcactcg cccgtcgcgg
     1381 ccgcttgtca ggttgccgtt gccgtcgcgg ctgtaaagcg tgccgtcagt gcccatgaca
     1441 tgccccggct caacaagcgc aggctgccgg ggctgtgctg gcgctacgac aatcggtgtg
     1501 ccggtgcgca caccttcgga caccgcgtga agccgggtcg cagcatcgcg taggctttcg
     1561 gcgttgcggg cgagtttctg cctgtcc
//
LOCUS       DAOQIE010000462         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_69198_length_1565_cov_4.754967, whole genome shotgun sequence.
ACCESSION   DAOQIE010000462 DAOQIE010000000
VERSION     DAOQIE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69198_length_1565_cov_4.754967"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..463)
                     /locus_tag="PK144_18220"
     CDS             complement(209..463)
                     /locus_tag="PK144_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNF67713.1"
                     /translation="MGFFSWILFGLIAGALAKLIMPGRQGGGFILTVLLGVVGALVGG
                     WMGTVMGFGTLSGFDLRSLGIAIAGALVVLFLFGLMRRSD"
     gene            557..1027
                     /locus_tag="PK144_18225"
     CDS             557..1027
                     /locus_tag="PK144_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67714.1"
                     /translation="MQPAPGLSLQRLPDTAAGLPRAAVLRHARPCGCVIDGAQLEAAL
                     CWPDGWTLLIASADCPFEESLHLSLLDAHDHVIDCAGIGMAYANGWFRELRLLPPDSV
                     RFGFLGNGEWSVRRLHTRGWRMPLFSEPVGVRRRFGWRRWFVIGRHESGNTGPD"
BASE COUNT          261 a          577 c          483 g          244 t
ORIGIN      
        1 catccgcgag gactccggcc cggtcaccaa ggatctgtcg cgcttcgtgc cgcacgtgat
       61 cctgaactga gccgcgcgcc ggctgccctg ccgtcccgcc tcggcatcgc tgcgcccgtc
      121 cctggcgggg atgcgtgcct ccccggacca gacgcccgcc cgcgcagccg ggagaaccgg
      181 cccgcggcag cgggcggtgg cgcagctttc agtccgatcg gcgcatcagg ccgaacagga
      241 acagcacgac cagcgcgccg gcgatggcga tgccgaggct gcgcaggtcg aagccgctca
      301 gcgtgccgaa gcccatgacc gtgcccatcc agccgccgac cagcgcgccg accacgccga
      361 gcagcacggt caggatgaag ccgccgccct ggcggccggg catgatgagt ttggcgagcg
      421 cgccggcgat caggccgaac aggatccagc tgaaaaagcc catggcgatg actcctggca
      481 gaagggttcg aggaaccggg cagcgttctg acgcaggatg ccggccggaa ttccccgccc
      541 gcccgcgccg gtgccgatgc agcccgctcc cgggctgagc ctgcagcgcc tgcccgacac
      601 ggccgccggc ctgccgcggg ccgccgtgct gcgccatgcc cgcccctgcg gctgcgtcat
      661 cgacggcgcg cagctcgaag ccgcgctgtg ctggccggat ggctggacgc tgctgatcgc
      721 cagtgccgat tgcccgttcg aggaatcgct gcatttgtcg ctgctcgatg cgcacgatca
      781 cgtcatcgat tgcgccggca tcggcatggc ttacgcaaac ggctggtttc gtgagctgcg
      841 cctgctgccc cccgacagcg tgcgcttcgg ctttctcggc aacggcgaat ggtcggtgcg
      901 ccgcctgcac acgcgcggct ggcggatgcc gctgttcagc gaacccgtcg gcgtgcggcg
      961 ccgcttcggc tggcggcgct ggttcgtgat cggacggcac gaatccggaa acaccggccc
     1021 ggactgaatc cggcaaccgg ggcgatttcc cgccggaatc agcgcatgcg cgcatcatcg
     1081 ccagccagac tgcaagcgga atgatctccg caagcccgat tcagcgccgg ccgcaagccc
     1141 tgcttgcgcc aataccgtca gcgtcaggac gaccttgcat tacgatatcg acacgcgcat
     1201 gaaaatcaga tgccccaaat ccaacgttcg ccgccgcctt tctacaagct gctgattcac
     1261 cgctataaat tcacaaaatc tccatatgcc atgaccactt gccacccgca ccggccagcg
     1321 gctagagtcc gggccgcaga gcattccggg cacgaccgtg aattcgccct gccagccgtt
     1381 ctgatcctgc ctgtccggcg ctgaagttcc ggcatcccga accaccgccc tgtatttccg
     1441 gccccggatt ccgcacgatt catccgcaac ggccctgcac gatcgaatcc ggcctgcgcc
     1501 ctgcccgcgc attgtccgca ttcacctgta ttcatctgca aacccgaagc cgtatctgca
     1561 aggga
//
LOCUS       DAOQIE010000463         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_111061_length_1224_cov_5.517536, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000463 DAOQIE010000000
VERSION     DAOQIE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111061_length_1224_cov_5.517536"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /gene="aceE"
                     /locus_tag="PK144_18230"
     CDS             <1..1085
                     /gene="aceE"
                     /locus_tag="PK144_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286208.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNF67715.1"
                     /translation="FRAVGIWNQQGQNYVPEDHDQLMFYKESKTGQVLQEGINEAGAM
                     SDWIAAATSYSVHNVQTIPFYICYSMFGLQRTLDLVWAAGDQRCRGFLVGGTAGRTTL
                     NGEGLQHEDGHSLILSNLVPNCVSYDPSFQYEVAVIVQDGLRRMYQEQEDIFYYITVM
                     NENYEHPEMPAGAEQDILKGMYQFRKGAASEGKKAAPRVQLLGSGTIFREVIAAAELL
                     KQDWGVDADIWGCPSMNELSRNGNDVSRWNLLNPAEKPKLSHVEACLNDTRGPVIAAT
                     DYVRLYSEQIRPFVNRRYVTLGTDGFGRSDTREKLRHFFEVNRYWVTLAALKALADDG
                     EIDRSKVAEAILKYGIDTNKPNPMSV"
     gene            1098..>1224
                     /locus_tag="PK144_18235"
     CDS             1098..>1224
                     /locus_tag="PK144_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67716.1"
                     /translation="MSNIVEVKVPDIGDFSDVPVIDVFVKPGDTVKVDDALVTLES"
BASE COUNT          252 a          382 c          388 g          202 t
ORIGIN      
        1 tgttccgcgc ggtcggcatc tggaaccagc agggccagaa ctacgtgccg gaagaccatg
       61 accagctgat gttctacaag gagtcgaaga ccggccaggt gctgcaggaa ggcatcaacg
      121 aagcgggtgc gatgagcgac tggatcgccg ccgccacttc gtactcggtg cacaacgtgc
      181 agacgatccc gttctacatc tgctactcga tgttcggcct gcagcgcacg ctggatctcg
      241 tctgggccgc cggcgaccag cgctgccgcg gcttcctggt cggcggcacc gccggtcgca
      301 cgacgctgaa cggcgaaggc ctgcagcatg aggacggcca cagcctgatc ctctcgaacc
      361 tggtgccgaa ctgcgtctcc tacgacccga gcttccagta cgaagtcgcg gtgatcgttc
      421 aggacggcct gcgtcgcatg taccaggagc aggaggacat cttctactac atcaccgtga
      481 tgaacgagaa ctacgaacat ccggagatgc cggccggcgc cgagcaggac atcctcaagg
      541 gcatgtacca gttcaggaag ggggctgcct cggaaggcaa gaaggccgca ccgcgcgtgc
      601 agctgctcgg atcgggcacg atcttccgcg aggtcatcgc cgccgccgaa ctgctcaagc
      661 aggactgggg tgtcgacgcc gacatctggg gctgcccgtc gatgaacgaa ctctcgcgca
      721 acggcaacga cgtctcgcgc tggaacctgc tcaacccggc cgagaagccg aagctgtcgc
      781 acgtcgaggc ctgcctcaac gacacgcgcg gaccggtgat cgccgcgacc gactacgtgc
      841 gactctactc ggagcagatc cgtccgttcg tcaatcgccg ctacgtgacg ctcggcaccg
      901 atggtttcgg tcgttcggac acgcgtgaaa agctgcgtca cttcttcgag gtgaatcgct
      961 actgggtcac gctggccgcg ctgaaggcat tggccgacga cggcgagatc gaccgcagca
     1021 aggtcgccga ggcgatcctg aagtacggga tcgataccaa caagccgaac ccgatgtcgg
     1081 tctaaggagg aaccagcatg agcaatatcg tggaagtgaa ggttccggac atcggtgact
     1141 tcagcgacgt gccggtcatc gacgtcttcg tgaagccggg tgacaccgtc aaggtggacg
     1201 atgcactggt caccctcgaa tcgg
//
LOCUS       DAOQIE010000464         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0634
            NODE_121493_length_1170_cov_5.589238, whole genome shotgun
            sequence.
ACCESSION   DAOQIE010000464 DAOQIE010000000
VERSION     DAOQIE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810266
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:40:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,649
            CDSs (total)                      :: 3,604
            Genes (coding)                    :: 3,585
            CDSs (with protein)               :: 3,585
            Genes (RNA)                       :: 45
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121493_length_1170_cov_5.589238"
                     /isolate="AMR_MDS_0634"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..647)
                     /locus_tag="PK144_18240"
     CDS             complement(39..647)
                     /locus_tag="PK144_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67717.1"
                     /translation="MYSVVTGRTSLRSSATPAPCRVLWLDACRRRPLPLRPFPPATRS
                     DPPMLRRLRSACLRACLSLLLMLTLPLQALAAVAPVWLPHGHAGLMAADGSVPAHRHA
                     LHAHAHGLGVHALTDGASASASASASELHGPDCAGHRHAAHEAAAGADDGDCGAHWFV
                     SAAMPTVAGALRLPPSRPVRHAGLVALHPLRLVDPPARPPRA"
     gene            723..>1170
                     /locus_tag="PK144_18245"
     CDS             723..>1170
                     /locus_tag="PK144_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF67718.1"
                     /translation="MPYDIIGDIHGQADKLLALLQRLGYRQRAGAWRHPQRQVIFVGD
                     YIDRGPAQLRTLDLVRRMTEAGSALAILGNHELNAIAWHTPDPRAPGEYLRPHFRPGR
                     GEKNRRQHAAFLAEVEHRPALHAELIDWFLSLPLWLELPGLRVVHAC"
BASE COUNT          188 a          417 c          436 g          129 t
ORIGIN      
        1 cgaaacccgc gccggcgttc gagccgggcg gtgcggggtc aggcgcgggg cgggcgggcg
       61 gggggatcga cgagtcgcag cggatgcagg gcgacgagac cggcgtggcg caccggccgg
      121 ctcggcggca gcctcagcgc gcccgcgacc gtcggcatcg cggcactgac gaaccagtgc
      181 gcgccgcaat cgccgtcgtc ggccccggca gcggcctcgt gggcagcgtg ccgatggccg
      241 gcacagtcag ggccgtgaag ctccgatgcc gatgccgatg ccgaggccga ggctccgtcg
      301 gtcagcgcat gcacgccgag cccgtgcgca tgcgcgtgca gcgcatgccg atgcgccggc
      361 accgaaccat cggccgccat cagccccgca tgcccgtgcg gcagccagac cggcgccacg
      421 gcggcgagcg cctgcagcgg cagggtcagc atcagcagca gggacaggca ggctcgcagg
      481 caggcggagc gcagacggcg cagcatcggc ggatcggatc gggtggccgg aggaaagggg
      541 cgtaacggta acggccggcg acggcaggcg tccagccata acacgcggca gggcgccggc
      601 gtggcagaag accgaaggct tgtcctgccg gtcacgacgg aatacaccgg ccgctcagcg
      661 ccttccccgc gcggggtcgg gcgacgcaag acaatcacgc atccacctgt gacgggcctg
      721 ccatgccata cgacatcatc ggcgacatcc acgggcaggc cgacaagctg ctggcgctgc
      781 tgcagcggct cggctaccgg cagcgggccg gggcctggcg acatccgcag cggcaggtga
      841 tcttcgtcgg cgactacatc gaccgcgggc cggcgcagct gcgcacgctg gacctcgtgc
      901 gccggatgac ggaagccgga tcggcgctgg cgatcctggg caaccacgaa ctgaacgcca
      961 tcgcctggca tacgcccgat ccccgcgcac cgggggaata cctgcgcccg catttccggc
     1021 cgggccgggg cgagaagaac cgccgccagc acgcggcctt cctcgccgaa gtcgaacacc
     1081 ggccggcact gcacgccgaa ctcatcgact ggttcctgag cctgccgctg tggctggagc
     1141 tgccgggcct gcgcgtcgtg catgcctgct
//
